bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved Li‐Ion Conduction and (Electro)Chemical Stability at Garnet‐Polymer Interface through Metal‐Nitrogen Bonding
Authors: Xu Yanan , Wang Kai , Zhang Xudong , Ma Yibo , Peng Qifan , Gong Yue , Yi Sha , Guo Hua , Zhang Xiong , Sun Xianzhong , Gao Hongcai , Xin Sen , Guo Yu‐Guo , Ma Yanwei 
Published: March 2023

AbstractOrganic‐inorganic composite solid electrolytes consisting of garnet fillers dispersed in polyvinylidene difluoride (PVDF) frameworks have shown promise to enable high‐energy solid‐state Li‐metal batteries. However, the air‐sensitive garnets easily form poorly‐conductive residues, which hinders fast Li‐ion exchange at the garnet‐polymer interface and results in low ionic conductivity. The highly alkaline residues trigger instant dehydrofluorination of PVDF to form unsaturated CC bonds, which are unstable against high‐voltage cathode materials. Here it is shown that, by applying a 10‐nm polydopamine coating on the residue‐removed garnet surface, the modified garnet filler becomes air‐stable and does not generate alkaline residues, so PVDF remains an intact structure. Surface characterizations reveal substantial metal‐nitrogen bonding between the La atoms of garnet and the amino groups of polydopamine, which can invite stronger adsorption of Li ions at the heterointerface. A new interparticle Li‐ion conduction mechanism is disclosed for the composite electrolyte, in which Li ions preferably migrate through the garnet‐polydopamine interface, forming an efficient ion‐percolation network. As a result, the composite electrolyte demonstrates an effective room‐temperature Li+ conductivity of 1.52 × 10–4 S cm–1 and a high cutoff voltage of up to 4.7 V versus Li+/Li to support stable operation of all‐solid‐state Li‐LiCoO2 batteries.
Sibling rivalry between twins in utero and childhood: Evidence from birthweight and survival of 95 919 twin pairs in 72 low‐ and middle‐income countries
Authors: Alemu Robel , Masters William A. , Finaret Amelia B. 
Published: March 2023

AbstractBackgroundThis study explores the magnitude and timing of sex and gender disparities in child development by describing differences in health outcomes for male and female siblings, comparing twins to control for all aspects of life circumstances other than sex and gender.MethodsWe construct a repeat cross‐sectional dataset of 191 838 twins among 1.7 million births recorded in 214 nationally representative household surveys for 72 countries between 1990 and 2016. To test for biological or social mechanisms that might favor the health of male or female infants, we describe differences in birthweights, attained heights, weights, and survival to distinguish gestational health from care practices after each child is born.ResultsWe find that male fetuses grow at the expense of their co‐twin, significantly reducing their sibling's birthweight and survival probabilities, but only if the other fetus is male. Female fetuses are born significantly heavier when they share the uterus with a male co‐twin and have no significant difference in survival probability whether they happen to draw a male or a female co‐twin. These findings demonstrate that sex‐specific sibling rivalry and male frailty begin in utero, prior to gender bias after birth that typically favors male children.ConclusionsSex differences in child health may have competing effects with gender bias that occurs during childhood. Worse health outcomes for males with a male co‐twin could be linked to hormone levels or male frailty, and could lead to underestimates of the effect sizes of later gender bias against girls. Gender bias favoring surviving male children may explain the lack of differences in height and weight observed for twins with either male or female co‐twins.
Ravulizumab in Aquaporin‐4–Positive Neuromyelitis Optica Spectrum Disorder
Authors: Pittock Sean J. , Barnett Michael , Bennett Jeffrey L. , Berthele Achim , de Sèze Jérôme , Levy Michael , Nakashima Ichiro , Oreja‐Guevara Celia , Palace Jacqueline , Paul Friedemann , Pozzilli Carlo , Yountz Marcus , Allen Kerstin , Mashhoon Yasmin , Kim Ho Jin 
Published: March 2023

ObjectiveCHAMPION‐NMOSD (NCT04201262) is a phase 3, open‐label, externally controlled interventional study evaluating the efficacy and safety of the terminal complement inhibitor ravulizumab in adult patients with anti–aquaporin‐4 antibody–positive (AQP4+) neuromyelitis optica spectrum disorder (NMOSD). Ravulizumab binds the same complement component 5 epitope as the approved therapeutic eculizumab but has a longer half‐life, enabling an extended dosing interval (8 vs 2 weeks).MethodsThe availability of eculizumab precluded the use of a concurrent placebo control in CHAMPION‐NMOSD; consequently, the placebo group of the eculizumab phase 3 trial PREVENT (n = 47) was used as an external comparator. Patients received weight‐based intravenous ravulizumab on day 1 and maintenance doses on day 15, then once every 8 weeks. The primary endpoint was time to first adjudicated on‐trial relapse.ResultsThe primary endpoint was met; no patients taking ravulizumab (n = 58) had an adjudicated relapse (during 84.0 patient‐years of treatment) versus 20 patients with adjudicated relapses in the placebo group of PREVENT (during 46.9 patient‐years; relapse risk reduction = 98.6%, 95% confidence interval = 89.7%–100.0%,p < 0.0001). Median (range) study period follow‐up time was 73.5 (11.0–117.7) weeks for ravulizumab. Most treatment‐emergent adverse events were mild/moderate; no deaths were reported. Two patients taking ravulizumab experienced meningococcal infections. Both recovered with no sequelae; one continued ravulizumab treatment.InterpretationRavulizumab significantly reduced relapse risk in patients with AQP4+ NMOSD, with a safety profile consistent with those of eculizumab and ravulizumab across all approved indications. ANN NEUROL 2023;93:1053–1068
Influence of Organic‐Cation Defects on Optoelectronic Properties of ASnI3 Perovskites A=HC(NH2)2, CH3NH3
Authors: Ji Qun , Wu Yilei , Gao Xinying , Zhang Tingbo , Zhou Yipeng , Zhang Yehui , Ju Ming‐Gang , Wang Jinlan , Zeng Xiao Cheng 
Published: March 2023

AbstractTin organic–inorganic halide perovskites (tin OIHPs) possess a desirable band gap and their power conversion efficiency (PCE) has reached 14 %. A commonly held view is that the organic cations in tin OIHPs would have little impact on the optoelectronic properties. Herein, we show that the defective organic cations with randomly dynamic characteristics can have marked effect on optoelectronic properties of the tin OIHPs. Hydrogen vacancies originated from the proton dissociation from FA [HC(NH2)2] in FASnI3 can induce deep transition levels in the band gap but yield relatively small nonradiative recombination coefficients of 10−15 cm3 s−1, whereas those from MA (CH3NH3) in MASnI3 can yield much larger nonradiative recombination coefficients of 10−11 cm3 s−1. Additional insight into the “defect tolerance” is gained by disentangling the correlations between dynamic rotation of organic cations and charge‐carrier dynamics.
Room‐Temperature‐Stable Diazoalkenes by Diazo Transfer from Azides: Pyridine‐Derived Diazoalkenes
Authors: Reitz Justus , Antoni Patrick W. , Holstein Julian J. , Hansmann Max M. 
Published: March 2023

AbstractRecently, stable diazoalkenes have received significant attention as a new substance class in organic chemistry. While their previous synthetic access was exclusively limited to the activation of nitrous oxide, we here establish a much more general synthetic approach utilizing a Regitz‐type diazo transfer with azides. Importantly, this approach is also applicable to weakly polarized olefins such as 2‐pyridine olefins. The new pyridine diazoalkenes are not accessible by the activation of nitrous oxide, allowing for a considerable extension of the scope of this only recently accessed functional group. The new diazoalkene class has properties distinct from the previously reported classes, such as photochemically triggered loss of dinitrogen affording cumulenes and not C−H insertion products. Pyridine‐derived diazoalkenes represent the so far least polarized stable diazoalkene class reported.
Self‐supporting flexible poly(1‐naphaneneboronic acid) film as green light‐emitting material
Authors: Kang Huan , Chao Shixing , He Yao , Liang Yanmei , Xu Jingkun , Zhou Weiqiang 
Published: March 2023

AbstractThe flexible conducting polymer films (FCPs) with fused ring structure is of great significance in the field of flexible electronics, yet their preparation is challenging. The mechanical properties can be improved by introducing boric acid groups to FCPs. Herein, free‐standing poly (1‐naphaneneboronic acid) (PNBA) film with a conductivity of 3 × 10−4 S cm−1 is readily prepared by electrochemical polymerization of 1‐naphaneneboronic acid. The PNBA film exhibits good redox activity and electrochemical stability. Fourier transform infrared spectrum proves that polymerization site of the as‐prepared PNBA occurs at C5 and C8 positions. The UV–Vis spectrum proves that the optical band gap of PNBA is 2.76 eV. In addition, thermal analysis reveals that the polymer displays excellent thermal stability. Fluorescence spectroscopy results show that PNBA film is a good green light‐emitting material. These characteristics indicate that free‐standing flexible PNBA film has potential applications in flexible electronic semiconductors, fluorescent materials and electrochemical sensors.
How reliable are Chinese hamster ovary (CHO) cell genome‐scale metabolic models?
Authors: Strain Benjamin , Morrissey James , Antonakoudis Athanasios , Kontoravdi Cleo 
Published: March 2023

AbstractGenome‐scale metabolic models (GEMs) possess the power to revolutionize bioprocess and cell line engineering workflows thanks to their ability to predict and understand whole‐cell metabolism in silico. Despite this potential, it is currently unclear how accurately GEMs can capture both intracellular metabolic states and extracellular phenotypes. Here, we investigate this knowledge gap to determine the reliability of current Chinese hamster ovary (CHO) cell metabolic models. We introduce a new GEM, iCHO2441, and create CHO‐S and CHO‐K1 specific GEMs. These are compared against iCHO1766, iCHO2048, and iCHO2291. Model predictions are assessed via comparison with experimentally measured growth rates, gene essentialities, amino acid auxotrophies, and 13C intracellular reaction rates. Our results highlight that all CHO cell models are able to capture extracellular phenotypes and intracellular fluxes, with the updated GEM outperforming the original CHO cell GEM. Cell line‐specific models were able to better capture extracellular phenotypes but failed to improve intracellular reaction rate predictions in this case. Ultimately, this work provides an updated CHO cell GEM to the community and lays a foundation for the development and assessment of next‐generation flux analysis techniques, highlighting areas for model improvements.
Prognosis and complications of patients with primary gastrointestinal diffuse large B‐cell lymphoma: Development and validation of the systemic inflammation response index‐covered score
Authors: Chu Yurou , Liu Yingyue , Jiang Yujie , Ge Xueling , Yuan Dai , Ding Mei , Qu Huiting , Liu Fang , Zhou Xiangxiang , Wang Xin 
Published: March 2023

AbstractBackgroundThis study aimed to evaluate the predictive value of systemic inflammation response index (SIRI) in primary gastrointestinal diffuse large B‐cell lymphoma (PGI‐DLBCL) patients and establish a highly discriminating risk prediction model.MethodsThis retrospective analysis included 153 PGI‐DCBCL patients diagnosed between 2011 and 2021. These patients were divided into a training set (n = 102) and a validation set (n = 51). Univariate and multivariate Cox regression analyses were conducted to examine the significance of variables on overall survival (OS) and progression‐free survival (PFS). An inflammation‐covered score system was established according to the multivariate results.ResultsThe presence of high pretreatment SIRI (≥1.34, p < 0.001) was significantly associated with poorer survival and identified as an independent prognostic factor. Compared with NCCN‐IPI, the prognostic and discriminatory capability of the novel model SIRI‐PI showed a more precise high‐risk assessment with a higher area under the curve (AUC) (0.916 vs 0.835) and C‐index (0.912 vs 0.836) for OS in the training cohort, and similar results were obtained in the validation cohort. Moreover, SIRI‐PI also showed good discriminative power for efficacy assessment. This new model identified patients at risk of developing severe gastrointestinal complications following chemotherapy.ConclusionsThe results of this analysis suggested that the pretreatment SIRI may be a potential candidate for identifying patients with a poor prognosis. And we established and validated a better‐performing clinical model, which facilitated the prognostic stratification of PGI‐DLBCL patients and can serve as a reference for clinical decision‐making.
Bioconversion of Ginsenoside Rf into Its Hydrated Derivative with Elevated Anti‐inflammatory Effect by a Highly Specific Biocatalytic System ofCordyceps Sinensis
Authors: Liu Jishuang , Xin Yu , Qiu Zhidong , Zhang Qi , He Tianzhu , Qiu Ye , Wang Weinan 
Published: March 2023

AbstractThe presence of 25‐OH moiety has been proved to enhance the bioactivity of dammarane saponins in many cases. However, such modification by previous strategies had compromised yield and purity of target products. Herein ginsenoside Rf was specifically transformed into 25‐OH‐(20S)‐Rf with a conversion rate of 88.03 % by aCordyceps Sinensis‐mediated biocatalytic system. The formulation of 25‐OH‐(20S)‐Rf was calculated by HRMS, whilst its structure was validated by1H‐NMR,13C‐NMR, HSQC, and HMBC analysis. Time‐course experiments unveiled straightforward hydration of the double bond on Rf with undetectable side reactions and maximum production of 25‐OH‐(20S)‐Rf on the 6thday, which collectively suggested the suitable timing of harvesting this target compound. In vitro bioassay of (20S)‐Rf and 25‐OH‐(20S)‐Rf against lipopolysaccharide‐induced macrophages indicated a significant boost of anti‐inflammatory effects after the C24−C25 double bond was hydrated. Therefore, the biocatalytic system in this article could be leveraged to deal with macrophage‐mediated inflammation under defined circumstances.
LC/MS/MS Phenolic Profile, Antioxidant Capacity, Angiotensin‐Converting Enzyme (ACE) and Glutathione S Transferase (GST) Inhibitory Properties of Ultrasound‐Assisted Propolis Extracts
Authors: Aras Abdülmelik , Korkmaz Tuğba , Bayrakdar Alpaslan 
Published: March 2023

AbstractResinous beehive product propolis has many biological activities. It contains various aromatic substances that have great differences in their chemical composition depending on the natural flora. Thus, chemical characterization and biological properties of propolis samples is an important subject for the pharmaceutical industry. In this study, the propolis samples collected from three cities in Turkey were prepared as methanol (MEP), ethanol (EEP), chloroform (ChlEP), hexane (HxEP), and ethyl acetate (EAEP) extracts using an ultrasonic assisted technique. The antioxidant capacities of the samples were evaluated by free radical scavenging activity (DPPH), cation radical scavenging activity (ABTS), and reducing activity (CUPRAC) and (FRAP). The strongest biological activities were detected in ethanol and methanol extracts. Enzyme inhibition of the propolis samples were determined against the human glutathione S‐transferase (GST) and angiotensin converting enzyme (ACE). IC50 values of MEP1, MEP2, and MEP3 samples against the ACE were found as 13.9 μg/mL, 14.8 μg/mL, and 12.8 μg/mL, while against the GST IC50 values of MEP1, MEP2, and MEP3 samples were as 5.92 μg/mL, 9.49 μg/mL, and 5.72 μg/mL. To know the possible causes of the biological test results advanced LC/MS/MS method was applied. trans‐ferulic acid, kaempferol, and chrysin were found as the most abundant phenolic compounds in each sample. The propolis extracts obtained using the proper solvent have a good potential to be used in pharmaceuticals to treat the diseases related to oxidative damage, hypertension, and inflammation. Finally, the interactions between chrysin, trans‐ferulic acid and kaempferol molecules with ACE and GST receptors were analyzed using molecular docking study. Selected molecules interact with active residues by binding to the active site of the receptors.
Development of an efficient method for selection of stable cell pools for protein expression and surface display with Expi293F cells
Authors: Chen Chuan , Wang Zening , Sun Zehua , Li Wei , Dimitrov Dimiter S. 
Published: March 2023

AbstractCompare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. However, selection of stable cell line is time‐consuming and laborious. Here we describe an optimized selection method to achieve high‐efficient stable cell pools with Expi293F suspension cells. This method only takes 2–3 weeks to generate stable cell pools with 2‐ to 10‐fold higher productivity than transient gene expression (TGE). In fed‐batch culture with Yeastolate, >1 g/L yield was achieved with our KTN0239‐IgG stable cell pool in shaker flasks. This method can be also applied to efficiently display proteins on the cell surface.
Resorc[4]arene Modifiers for Supramolecular Site‐Directed Immobilization of Antibodies on Multi‐Walled Carbon Nanotubes
Authors: Polli Francesca , Cianfoni Gabriele , Elnahas Rem , Mangiardi Laura , Scaramuzzo Francesca A. , Cammarone Silvia , Quaglio Deborah , Calcaterra Andrea , Pierini Marco , Mazzei Franco , Zanoni Robertino , Botta Bruno , Ghirga Francesca 
Published: March 2023

AbstractOne of the main problems in developing immunosensors featuring carbon nanotubes (CNTs) is immobilizing antibodies (Abs) onto the CNT surface to afford selective binding to target antigens (Ags). In this work, we developed a practical supramolecular Ab conjugation strategy based on resorc[4]arene modifiers. To improve the Ab orientation on the CNTs surface and optimizing the Ab/Ag interaction, we exploited the host‐guest approach by synthesizing two newly resorc[4]arene linkers R1 and R2 via well‐established procedures. The upper rim was decorated with eight methoxyl groups to promote selective recognition of the fragment crystallizable (Fc) region of the Ab. Moreover, the lower rim was functionalized with 3‐bromopropyloxy or 3‐azidopropiloxy substituents to bind the macrocycles on the multi‐walled carbon nanotubes (MWCNTs) surface. Accordingly, several chemical modifications of MWCNTs were evaluated. After the morphological and electrochemical characterization of nanomaterials, the resorc[4]arene‐modified MWCNTs were deposited onto a glassy carbon electrode surface to evaluate their potential applicability for label‐free immunosensor development. The most promising system showed an improved electrode active area (AEL) of almost 20 % and a site‐oriented immobilization of the SARS‐CoV‐2 spike protein S1 antibody (Ab‐SPS1). The developed immunosensor revealed a good sensitivity (23.64 μA mL ng−1 cm−2) towards the SPS1 antigen and a limit of detection (LOD) of 1.01 ng mL−1.
Capturing Covalent Catalytic Intermediates by Enzyme Mutants: Recent Advances in Methodologies and Applications
Authors: Wang Yongchao , Tang Shan 
Published: March 2023

AbstractCovalent catalytic intermediates provide valuable information for revealing the catalytic mechanism, probing the enzyme activity and identifying substrate specificity. However, naturally formed covalent intermediates are too rapidly degraded for general biological studies. In order to capture transient covalent intermediates, a variety of chemical strategies have been developed over decades to extend the half‐life of the enzyme‐substrate intermediates (or close analogues) required for the downstream structural and functional studies. This review summarizes three general mechanism‐based strategies for trapping covalent catalytic intermediates. In particular, enzyme mutant‐based approaches, especially the introduction of genetically encoded 2,3‐diaminopropionic acid to replace the catalytic cysteine/serine in proteases for acyl‐enzyme intermediate trapping are described. In addition, the applications of trapped intermediates in structural, functional and protein labeling studies are presented, and the potential new directions of using enzyme substrate traps are discussed at the end of the review.
Pulmonary adenocarcinoma mimicking miliary tuberculosis in a 20‐year‐old man: A clinical case report
Authors: Seifi Arash , Shafiee Nahid , Moradi Maryam 
Published: March 2023

AbstractLung adenocarcinoma (LA) is the most common subtype of lung cancer with nonsignificant manifestations. Some benign conditions can mimic LA in symptoms and even chest imaging.In this case report, we are discussing a young man without any significant medical history with metastatic LA, initially presumed military TB.
Effects of Group IB Metals of Au/Ag/Cu on Boosting Oxygen Evolution Reaction of Cobalt Hydroxide
Authors: Wang Xianchao , Zang Zehao , Fan Chunyan , Zhang Yintao , Li Xiang , Li Lanlan , Yu Xiaofei , Yang Xiaojing , Lu Zunming , Zhang Xinghua 
Published: March 2023

AbstractDeveloping high‐activity, good‐stability oxygen evolution reaction (OER) catalysts is the key to solving the problem of hydrogen production from electrolytic water. Cobalt hydroxide (Co(OH)2) is a hopeful OER catalyst, but its poor conductivity and low inherent activity limit its OER performance. Herein, we used group IB metals of Au, Ag, and Cu to increase the OER performance of Co(OH)2via the electrodeposition method. All three metals can increase the carrier concentration and proportion of high‐valence cobalt ions. The analysis results disclose that the construction of Ag−Co(OH)2heterostructure can optimize the electronic structure through interfacial interactions, produce a moderate proportion of high‐valence cobalt centers, enhance the charge transport capacity and the adsorption of hydroxyl species, thus accelerating the OER kinetics and effectively improving the inherent catalytic activity. This work not only develops effective and steady catalysts but also provides a facile method to enhance the OER performance through interface engineering.
Staubfreies Absacken von Carbon Black
Authors: Pohl Sebastian 
Published: March 2023

AbstractFür die Herstellung von Primär‐ als auch Sekundärbatterien wird Carbon Black benötigt, welches schwer abzusacken ist und hohe Logistik‐ und Qualitätskosten verursacht. Die Vakuumtechnologie des Verpackungsmaschinenherstellers Greif‐Velox löst diese Probleme und sorgt dafür, dass der wertvolle Rohstoff für Batteriehersteller effizienter und staubfrei in größeren Mengen verfügbar gemacht werden kann.
Kombinierte CO‐Detektion und Feuchtemessung
Authors: Kemmling Alexander , Kendik Marcus 
Published: March 2023

AbstractTrocknungsprozesse unterliegen einem hohen Risiko für eine Staubexplosion und der vorbeugende Explosionsschutz hat einen hohen Stellenwert. Bei der gefährlichen Selbstentzündung von Stäuben entstehen Glimmnester, die Kohlenmonoxid freisetzen. Außerdem ist die Luftfeuchte ein Gefahrenindikator. Das Messsystem CO.Pilot von Rembe misst beide Parameter in Echtzeit und trägt damit zum Explosionsschutz und zu einem optimierten Trocknungsprozess bei.