bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C3 and C4 grasses
Authors: Billakurthi Kumari , Hibberd Julian M. 
Published: July 2023

Abstract
Background
It has been proposed that engineering the C4 photosynthetic pathway into C3 crops could significantly increase yield. This goal requires an increase in the chloroplast compartment of bundle sheath cells in C3 species. To facilitate large-scale testing of candidate regulators of chloroplast development in the rice bundle sheath, a simple and robust method to phenotype this tissue in C3 species is required.

Results
We established a leaf ablation method to accelerate phenotyping of rice bundle sheath cells. The bundle sheath cells and chloroplasts were visualized using light and confocal laser microscopy. Bundle sheath cell dimensions, chloroplast area and chloroplast number per cell were measured from the images obtained by confocal laser microscopy. Bundle sheath cell dimensions of maize were also measured and compared with rice. Our data show that bundle sheath width but not length significantly differed between C3 rice and C4 maize. Comparison of paradermal versus transverse bundle sheath cell width indicated that bundle sheath cells were intact after leaf ablation. Moreover, comparisons of planar chloroplast areas and chloroplast numbers per bundle sheath cell between wild-type and transgenic rice lines expressing the maize GOLDEN-2 (ZmG2) showed that the leaf ablation method allowed differences in chloroplast parameters to be detected.

Conclusions
Leaf ablation is a simple approach to accessing bundle sheath cell files in C3 species. We show that this method is suitable for obtaining parameters associated with bundle sheath cell size, chloroplast area and chloroplast number per cell.
Risk factors for postoperative myocardial injury-related cardiogenic shock in patients undergoing cardiac surgery
Authors: Cheng Xiao-Feng , Wang Kuo , Zhang Hai-Tao , Zhang He , Jiang Xin-Yi , Lu Li-Chong , Chen Cheng , Cheng Yong-Qing , Wang Dong-Jin , Li Kai 
Published: July 2023

Abstract
Background
Myocardial injury-related cardiogenic shock (MICS) is significantly associated with poor outcomes in patients after cardiac surgery. Herein, we aimed to investigate the risk factor for postoperative MICS.

Methods
We performed a case-control study on 792 patients undergoing cardiac surgery from 2016 to 2019, including 172 patients with postoperative MICS and 620 age- and sex-matched controls. MICS was defined as composite criteria: a cardiac index of < 2.2 L/m2/min, arterial lactate levels of > 5 mmol/L at the end of the surgery, a vasoactive-inotropic score of > 40 at the end of the surgery, and a cardiac troponin T (cTnT) level of > 0.8 µg/L on postoperative day 1 (POD1) with an increase of > 10% on POD 2.

Results
A total of 4671 patients who underwent cardiac surgery in our hospital between 2016 and 2019 were included; of these, 172 (3.68%) had MICS and the remaining 4499 did not. For investigating the risk factors, we selected 620 age- and sex-matched controls. In the univariate analysis, MICS was significantly associated with death (P < 0.05), extracorporeal membrane oxygenation (P < 0.05), continuous renal replacement therapy (P < 0.01), and ventricular arrhythmias (P < 0.05). Multivariable logistic regression analysis revealed that diabetes mellitus (OR:8.11, 95% CI: 3.52–18.66, P < 0.05) and a cardiopulmonary bypass (CPB) time of > 2 h (OR: 3.16, 95% CI: 1.94–5.15, P < 0.05) were associated with postoperative MICS. Moreover, long-time administration of preoperative calcium channel blocker (CCB) was associated with a less incidence of MICS (OR: 0.11, 95% CI: 0.05–0.27, P < 0.05).

Conclusions
Postoperative MICS is significantly associated with poor outcomes. Diabetes mellitus and long CPB time are associated with MICS. Preoperative CCB administration is associated with less incidence of MICS.
Association of dietary intake and cervical cancer: a prevention strategy
Authors: Nazari Elham , Hasanzadeh Malihe , Rezvani Reza , Rejali Marzieh , Badpeyma Mohaddeseh , Delaram Zeinab , Mousavi-Seresht Leila , Akbari Mahdieh , Khazaei Majid , Ferns Gordon A. , Avan Amir 
Published: July 2023

Abstract
Introduction
Cervical cancer is one of lethal cancers in women. As a global concern, identifying important factors of cancer is a useful strategy for prevention. Due to the role of diet/nutrition factors for cancer, the purpose of our study was to determine the impact of 150 nutrition/vitamin factors and 50 non-nutritional factor in cervical cancer and phase.

Methods
Population samples of 2088 healthy subjects and patients with cervical cancer were investigated. 200 factors such as vitamin E, B1, B6, fruits, HPV, and age were gathered. Deep learning, Decision tree, and correlation matrix were used for modeling and identifying important factors. SPSS 26, R4.0.3, and Rapid miner were utilized for implementation.

Results
Our findings indicated that zinc, Iron, Niacin, Potassium, Phosphorous, and Cooper have a beneficial impact in reducing the risk of cervical cancer and progression of phase in Iranian women, as well as Salt, snacks and milk Were identified as high-risk food factors (P value < 0.05 and coefficient correlation > 0.6). Also, alcohol, and sex patient with two groups, HPV positive have an impact on cervical cancer incidence. Phosphorus and selenium in the Micronutrients category (R2 = 0.85, AUC = 0.993) and polyunsaturated fatty acid and salt in the Macronutrients category and other categories of nutrients were identified as the most effective factors in cervical cancer using deep learning (R2 = 0.93, AUC = 0.999).

Conclusions
A diet and rich nutrition can be helpful for the prevention of cervix cancer and may reduce the risk of disease. Additional research is necessary for different countries.
Clinical outcomes of surgical treatment for Copenhagen syndrome: a case series
Authors: Safaei Saeid , Azimi Parisa , Yazdanian Taravat 
Published: July 2023

Abstract
Background
Copenhagen syndrome (CS) is a rare disorder mostly observed in adolescent. The onset of the disease, with a progressive anterior vertebral ankylosis in the thoracic and/or lumbar areas often clinically revealed by thoracolumbar kyphosis. We report a series of three patients of CS with good outcome.

Case presentation
The mean age of patients were 14.0 (SD = 3.6) years at admission time. Patients underwent clinical and radiological examination (MRI, CT scan, and bone scan) before surgery and revealed Copenhagen syndrome. Case 2 received conservative treatment braces and regular follow-up. Finally, all patients were treated according to their clinical conditions through a combined surgical approach such as pedicle subtraction osteotomy (PSO), ponte osteotomy, hook, pedicular screw insertion, and fusion. In postoperative follow-up, the deformity correction was achieved with proper alignment in all the cases.

Conclusion
The treatment of CS with PSO plus ponte osteotomy seems to result in an excellent surgical procedure and outcome for our patients based on deformity severity. Bone scan imaging could be considered as an aid to differential diagnosis, which is an effective method.
File processing security detection in multi-cloud environments: a process mining approach
Authors: Zhang Xiaolu , Cui Lei , Shen Wuqiang , Zeng Jijun , Du Li , He Haoyang , Cheng Long 
Published: July 2023

AbstractCloud computing has gained popularity in recent years, but with its rise comes concerns about data security. Unauthorized access and attacks on cloud-based data, applications, and infrastructure are major challenges that must be addressed. While machine learning algorithms have improved intrusion detection systems in cloud data security, they often fail to consider the entire life cycle of file processing, making it difficult to detect certain issues, especially insider attacks. To address these limitations, this paper proposes a novel approach to analyzing data file processing in multi-cloud environments using process mining. By generating a complete file processing event log from a multi-cloud environment, the proposed approach enables detection from both control flow and performance perspectives, providing a deeper understanding of the underlying file processing in its full life cycle. Through our case study, we demonstrate the power and capabilities of process mining for file security detection and showcase its ability to provide further insights into file security in multi-cloud environments.
Semi-automated quantification of tricuspid valve dynamics and structure in tetralogy of Fallot and hypoplastic left heart syndrome using three-dimensional echocardiography
Authors: Jani Vivek , Li Ling , Craft Mary , Veronesi Federico , Khoo Nee , Danford David , Muraru Denisa , Kutty Shelby 
Published: July 2023

AbstractAnomalies of the tricuspid valve (TV) are associated with worsened prognosis in congenital heart disease (CHD). Here, we present a descriptive study examining changes in TV morphology in two CHD conditions—repaired tetralogy of Fallot (rTOF) and hypoplastic left heart syndrome (HLSH), using three-dimensional echocardiography. Full volume acquisitions of the TV and right ventricle (RV) were performed from an RV-focused apical view using ECG gating over 2–5 consecutive cardiac cycles using 3D echocardiography, from which TV annulus and leaflet parameters were quantified. A total of 40 rTOF patients (age 14 ± 9.8 years), 40 HLHS patients (age1.0 ± 1.5 years) and 80 age and gender matched controls were included. Among leaflet parameters, antero-posterior and posterior-septal TV coaptation heights were smaller in rTOF (p < 0.001) vs. control. Conversely, only the short-axis TV height was different in HLHS vs. controls (HLHS 1.6 ± 0.4 cm vs. control 1.4 ± 0.3 cm). TV leaflet parameters tended to be larger in HLHS, while leaflet coaptation distances were similar between groups. We demonstrate that 3D echocardiography for assessment of the TV is feasible in rTOF and HLHS patients and identifies unique differences in TV morphology. Future studies should clarify the clinical significance of TV morphology in these patient populations.
Pediatric Precision Medicine at the National Cancer Center Japan: Prospective Genomic Study of Pediatric Patients with Cancer as Part of the TOP-GEAR Project
Authors: Tao Kayoko , Yamazaki Fumito , Kubo Takashi , Sunami Kuniko , Kumamoto Tadashi , Arakawa Ayumu , Sugiyama Masanaka , Watanabe Yuko , Nakajima Miho , Shirakawa Nami , Tanimura Kazuki , Koyama Takafumi , Hirata Makoto , Sudo Kazuki , Tanabe Noriko , Watanabe Tomoko , Yoshida Teruhiko , Kitami Mayuko , Yoshida Akihiko , Yatabe Yasushi , Nakano Yoshiko , Ohira Miki , Kamijo Takehiko , Nakazawa Atsuko , Kato Motohiro , Ichimura Koichi , Kohno Takashi , Yamamoto Noboru , Hishiki Tomoro , Ichikawa Hitoshi , Ogawa Chitose 
Published: July 2023

PURPOSE This single-center, prospective molecular profiling study characterizes genomic alterations and identifies therapeutic targets in advanced pediatric solid tumors. METHODS As part of the TOP-GEAR (Trial of Onco-Panel for Gene profiling to Estimate both Adverse events and Response by cancer treatment) project at the National Cancer Center (NCC), Japan, we enrolled pediatric patients with a refractory or recurrent disease during August 2016-December 2021 and performed genomic analysis of matched tumors and blood using originally developed cancer gene panels, NCC Oncopanel (ver. 4.0) and NCC Oncopanel Ped (ver. 1.0). RESULTS Of 142 patients (age, 1-28 years) enrolled, 128 (90%) were evaluable for genomic analysis; 76 (59%) patients harbored at least one reportable somatic or germline alteration. The tumor samples were collected during the initial diagnosis in 65 (51%) patients, after treatment initiation in 11 (9%) patients, and upon either disease progression or relapse in 52 (41%) patients. The leading altered gene was TP53, followed by MYCN, MYC, CDKN2A, and CDK4. The commonly affected molecular processes were transcription, cell-cycle regulation, epigenetic modifiers, and RAS/mitogen-activated protein kinase signaling. Twelve (9%) patients carried pathogenic germline variants in cancer-predisposing genes. Potentially actionable findings were identified in 40 (31%) patients; to date, 13 (10%) patients have received the recommended therapy on the basis of their genomic profiles. Although four patients had access to targeted therapy through clinical trials, the agents were used in nine patients in an off-label setting. CONCLUSION The implementation of genomic medicine has furthered our understanding of tumor biology and provided new therapeutic strategies. However, the paucity of proposed agents limits the full potential of actionability, emphasizing the significance of facilitating access to targeted cancer therapies.
Hydra morphogenesis as phase transition dynamics
Authors: Agam Oded , Braun Erez 
Published: July 2023

Abstract
We utilize whole-body Hydra regeneration from a small tissue segment to develop a physics framework for animal morphogenesis. Introducing experimental controls over this process, an external electric field and a drug that blocks gap junctions, allows us to characterize the essential step in the morphological transition —from a spherical shape to an elongated spheroid. We find that spatial fluctuations of the Ca2+ distribution in the Hydra's tissue drive this transition and construct a field-theoretic model that explains the morphological transition as a first-order–like phase transition resulting from the coupling of the Ca2+ field and the tissue's local curvature. Various predictions of this model are verified experimentally.
Epigenetic Dysregulation in Endometriosis: Implications for Pathophysiology and Therapeutics
Authors: Marquardt Ryan M , Tran Dinh Nam , Lessey Bruce A , Rahman Md Saidur , Jeong Jae-Wook 
Published: July 2023

Abstract
Endometriosis is a prevalent gynecological condition associated with pelvic pain and infertility. Despite more than a century of research, the etiology of endometriosis still eludes scientific consensus. This lack of clarity has resulted in suboptimal prevention, diagnosis, and treatment options. Evidence of genetic contributors to endometriosis is interesting but limited; however, significant progress has been made in recent years in identifying an epigenetic role in the pathogenesis of endometriosis through clinical studies, in vitro cell culture experiments, and in vivo animal models. The predominant findings include endometriosis-related differential expression of DNA methyltransferases and demethylases, histone deacetylases, methyltransferases, and demethylases, and regulators of chromatin architecture. There is also an emerging role for miRNAs in controlling epigenetic regulators in the endometrium and endometriosis. Changes in these epigenetic regulators result in differential chromatin organization and DNA methylation, with consequences for gene expression independent of a genetic sequence. Epigenetically altered expression of genes related to steroid hormone production and signaling, immune regulation, and endometrial cell identity and function have all been identified and appear to play into the pathophysiological mechanisms of endometriosis and resulting infertility. This review summarizes and critically discusses early seminal findings, the ever-growing recent evidence of epigenetic contributions to the pathophysiology of endometriosis, and implications for proposed epigenetically targeted therapeutics.
La lucha contra la trata de esclavos en el Caribe: Los Tribunales del Vicealmirantazgo británicos, 1807–1825
Authors: Bachero Bachero Juan Luis 
Published: July 2023

AbstractLa lucha británica contra la trata de esclavos tuvo un punto clave y bien conocido en Sierra Leona, pero hubo otros tribunales en distintas colonias británicas del Caribe por los que pasaron miles de esclavos. Su ubicación les otorgó una mayor versatilidad, así que no actuaron únicamente contra la esclavización de africanos, sino que liberaron a esclavos huidos de otras colonias y persiguieron la pequeña trata intercaribeña y los traslados de esclavos no debidamente documentados. El artículo explica la actividad de los Tribunales del Vicealmirantazgo (Vice-Admiralty Courts), esenciales para entender la estructura británica de lucha contra la trata entre 1807 y 1817. Asimismo, explica su organización posterior a 1817 a través de las Comisiones Mixtas, sucesoras de los tribunales caribeños y cuya configuración estuvo muy condicionada por éstos. En el caso de las Comisiones Mixtas, la centralización les otorgó mayor eficiencia en su cometido y su ubicación fuera de las posesiones británicas les permitió deshacerse de la presencia indeseada de emancipados.
Bilateral preventive mastectomy as a preventive method of breast cancer in the opinion of women
Authors: Olejniczak-Nowakowska Małgorzata , Trocha Weronika , Krupa-Kotara Karolina , Grajek Mateusz 
Published: July 2023

Background
Breast cancer is the most common cancer among women. Despite of the progress of medicine and develop of treatment, it is still the second couse of death among women. To reduce the risk of getting breast cancer, the more ofen teratement is "preventive mastectomies" which completely remove the breast gland by healty women that are in the "high risk" group.
Material and Methods
To conduct the research I develop a survey, written by my own. The study included 252 women from Poland over the age of 18.
Results
The opinion about the performance of preventive mastectomies in case of women in "high risk" was very diverse. Only 13% of interviewees were convinced that they would have the surgery and up to 29% could not give an answer. Most woman (86%) are convinced that breast cancer is genetically determined. That mastectomy can be a way to prevent breast cancer agrees 69% of respondents. More than half of respondents (52%) don't know if such surgery is in Poland available. Only half of the respondents could indicate the place where an examination of an increased risk of breast cancer can be made (49%). 81% of respondents examine there breasts at least with one method, and 19% did not check it at all.
Conclusions
The knowledge about the heredity of cancer and the availability of treatments is insufficient. Women are not convinced that they would have gone to radical preventive treatment, and they are afraid of many consequences connected with it. It is essential to educate the public including the medical stuff in this scope. It is also important that the patient together with his doctor examined all the possible options for limiting the risk of cancer.
Vitamin D – knowledge among people about its properties and effects based on anonymous surveys
Authors: Grużewska Agnieszka , Grużewska-Piotrowska Kinga 
Published: July 2023

Introduction: Vitamin D is produced under the influence of solar radiation. It can also be supplied in food or dietary supplements. The assessment of total serum 25(OH)D is widely accepted as an indicator of vitamin D concentration and observation of the population regarding hypovitaminosis or recommendations regarding supplementation. In fact, the definition of vitamin D deficiency is still a contentious issue, however, epidemiological data indicate that vitamin D deficiency is widespread worldwide, including Poland.
The aim of the study: The aim of the study was to assess the knowledge of people about supplementation and the effects of vitamin D, its deficiency and consumed nutrients, in which it is found, based on anonymous surveys.
Materials and methods: The research material was collected using an anonymous online survey in April and May 2023. The obtained results were analyzed and verified on the basis of scientific literature and statistically processed using Microsoft Office Excel.
Results: 47.3% of the respondents take dietary supplements with vitamin D, but 76% of these people do not know the dose they are taking. More than half of them declare knowledge of the function of vitamin D3, of which 70.3% of respondents correlate the effect of cholecalciferol with the skeletal system. 50.2% of people eat fish rarely or not at all. 68.5% of respondents claim that vitamin D should be supplemented with vitamin K to be effective.
Discussion and conclusions: Less than half of the respondents take preparations containing vitamin D. Few people know the full use of vitamin D in the body and its multidirectionality. More than half of the respondents do not know the effects of hypovitaminosis D. Prevention of vitamin D deficiency should be reintroduced due to lifestyle changes, e.g. after the COVID-19 pandemy.
Keywords: vitamin D, hypovitaminosis, cholecalciferol, calcium
Hyaluronic acid and its use in non-hormonal therapy for the treatment of vaginal atrophy and the level of awareness about the disease itself among the women from the Podkarpackie Voivodeship
Authors: Glac Agata , Musz Kinga , Dobko Katarzyna , Frącz Gabriela , Kuliga Klaudia , Sejnowska Natalia , Socha Agnieszka , Surowiec Agata , Śnieżek Kinga , Zając Anna 
Published: July 2023

Introduction: Hyaluronic acid is the ingredient contained in the composition that has the ability to bind water, found in the cornea, synovial fluid and cartilage stroma. Its properties are suitable for use in an increasing range of fields, both aesthetic and in treatments focusing on the treatment of vaginal atrophy.
Aim of the study: The aim of the study was to collect and analyze publications on the use of hyaluronic acid in the treatment of vaginal atrophy and assessment of the level of knowledge among women about this disease.
Materials and methods: The research material was collected using an anonymous online survey. The obtained results were analyzed and verified on the basis of scientific literaturę and statistically processed using Microsoft Office Excel.
Results: 59,7% of the respondents knew the term "vaginal atrophy" and 64,2% were able to correctly determine which of the hormones (estrogen) is the main cause of this condition. 59,7% of women indicated that they regularly undergo examinations and perform cytology every year, 29,9% indicated that they have examinations and visits "once in 2 years", 6% - "once in 3 years", the answers "less than once in 3 years” was given by 4,5% of the respondents. 85,7% of the surveyed women have never noticed any symptoms of vaginal atrophy and 85,9% believe that there are effective forms of treating this disorder.
Conclusions: Awareness among women about vaginal atrophy, both those with medical education and women in other professions, is at a moderate level. Many women do not talk to their doctor about this not because of the feeling of shame, but also because of the lack of information and preventive measures. The progressive nature of vaginal atrophy should encourage clinicians to routinely engage in an open dialogue with patients about genitourinary health.