bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Dialogic Approach to Enhancing Argumentative Writing From Multiple Texts
Authors: Shi (石雨晨) Yuchen , Iordanou Kalypso 
Published: January 2025

Purpose The study sought to examine the effectiveness of a dialogue-based argument intervention in enhancing Chinese middle school students’ integration of conflicting information from multiple texts in argumentative writing. Design/Approach/Methods The study followed a quasi-experimental design with pre-assessment and post-assessment, comparing seventh-grade intervention and non-participating control students’ individual post-assessment writing performance on a non-discourse topic involving genetically modified foods. Findings Intervention students outperformed control students in integrating textual evidence inconsistent with one's position. Specifically, intervention students were more successful in integrating position-inconsistent information with their prior knowledge or integrating multiple pieces of position-inconsistent information from one text or across multiple texts. Intervention students were also more successful in integrating two pieces of conflicting information. When judging text trustworthiness, intervention students trusted a primary source to a greater extent and showed greater gains in taking into consideration the epistemological aspect, as well as one's own or a text's position on the issue. Originality/Value The present study demonstrated the effectiveness of the dialogue-based argument curriculum in promoting Chinese middle school students’ ability to write integrated essays from multiple texts.
Feedback loop centered on MAF1 reduces blood–brain barrier damage in sepsis-associated encephalopathy
Authors: Wei Xuebiao , Jiang Wenqiang , Wang Zhonghua , Li Yichen , Jing Yuanwen , Han Yongli , Huang Linqiang , Chen Shenglong 
Published: January 2025

Abstract

Background
A previous study found that MAF1 homolog, a negative regulator of RNA polymerase III (MAF1), protects the blood–brain barrier (BBB) in sepsis-associated encephalopathy (SAE); however, the related molecular mechanisms remain unclear.

Subjects and methods
In this study, a rat sepsis model was constructed using the cecum ligation and puncture (CLP) method. In vitro, rat brain microvascular endothelial cells and astrocytes were stimulated with serum from the sepsis model rats. The loss of MAF1 protein levels and the molecular mechanisms leading to cell damage were investigated.

Results
It was shown in the SAE models that MAF1 was expressed at low levels. Knockdown of Cullin 2 (CUL2) stimulated the accumulation of MAF1 protein, attenuated the RNA sensor RIG-I/interferon regulatory factor 3 (IRF3) signaling pathway, and reduced cell apoptosis. Furthermore, it increased phosphatase and tensin homolog (PTEN) expression and inactivated the serine/threonine kinase (AKT)/mechanistic target of the rapamycin kinase (mTOR) signaling pathway. Interference with forkhead box O1 (FOXO1) inhibited MAF1 expression and activated the RIG-I/IRF3 signaling pathway, while MAF1 overexpression promoted PTEN expression, decreased cell apoptosis, and normalized autophagy.

Conclusions
These findings demonstrate that CUL2 promoted MAF1 ubiquitination and caused BBB injury in SAE. Through the regulatory loop of PTEN/AKT/FOXO1/MAF1, CUL2 initiated the gradual downregulation of MAF1, which subsequently regulated polymerase III (Pol III)-dependent transcription and played essential roles in cell apoptosis in SAE.

Clinical trial number: not applicable.

Graphical Abstract
Beyond destruction: emerging roles of the E3 ubiquitin ligase Hakai
Authors: Escuder-Rodríguez Juan-José , Rodríguez-Alonso Andrea , Jove Lía , Quiroga Macarena , Alfonsín Gloria , Figueroa Angélica 
Published: January 2025

Abstract
Hakai protein (CBLL1 gene) was identified as an E3 ubiquitin ligase of E-cadherin complex, inducing its ubiquitination and degradation, thus inducing epithelial-to-mesenchymal transition. Most of the knowledge about the protein was associated to its E3 ubiquitin ligase canonical role. However, important recent published research has highlighted the noncanonical role of Hakai, independent of its E3 ubiquitin ligase activity, underscoring its involvement in the N
6-methyladenosine (m6A) writer complex and its impact on the methylation of RNA. The involvement of Hakai in this mRNA modification process has renewed the relevance of this protein as an important contributor in cancer. Moreover, Hakai potential as a cancer biomarker and its prognostic value in malignant disease also emphasize its untapped potential in precision medicine, which would also be discussed in detail in our review. The development of the first small-molecule inhibitor that targets its atypical substrate binding domain is a promising step that could eventually lead to patient benefit, and we would cover its discovery and ongoing efforts toward its use in clinic.

Graphical Abstract
Electroporation assisted delivery of Roussin salt porphyrin-based conjugated carbon nanoparticles for sono–X-ray–photodynamic prostate cancer in vitro and in vivo treatment
Authors: Abd El-Kaream Samir Ali , Mohamad Abed Elrahman Ahmad , El-Kholey Sohier Mahmoud , Ebied Samia Abd El-Moniem 
Published: January 2025

Abstract

Background
In the realm of cancer treatment, sono–X-ray–photodynamic therapy (SXPDT) has garnered significant interest as a novel therapeutic approach. The essential part of SXPDT is the sensitizer, which under X-ray photon and ultrasound sono-irradiation may transform sono and photo-energy into cytotoxic molecules. Photon absorption, targeting, penetration, and oxygen dependence remain challenges in sono–X-ray–photosensitizer (SXPs) design. Rapid advancements in material science have prompted the creation of several SXPs that create cytotoxic species with great selectivity, safety, and noninvasiveness for the treatment of tumors. The current study aims to provide an advanced method of activated cancer treatment by using electroporation to assist the delivery of Roussin salts porphyrin-based conjugated carbon nanoparticles (EP@RRBP-CNP) for the sono–X-ray–photodynamic prostate cancer (PCa) in vivo and in vitro treatment.

Materials and methods
Human PCa cells (DU-145) were used in the in vitro study, and the in vivo application groups of the study protocol were Swiss albino mice treated with N-methyl-N-nitrosourea (MNU) / testosterone only; they were not given any treatment to induce PCa. The study treatment protocol started only after PCa induction, and involved daily administration of EP@RRBP-CNP as SXPDT sensitizer whether or not to be exposed to photo–(X-ray) or sono–(US) or a combination of them for 3 min for a period of 2 weeks.

Results
Indicated that CNP is a useful RRBP delivery mechanism that targets PCa cells directly. Furthermore, EP@RRBP-CNP is a promising SXPS that, when used in conjunction with SXPDT, can be very effective in in vitro treating PCa-DU-145 (in a dose-dependent manner cell viability declined, an increase in the cells population during the G0/G1-phase indicates that the cell cycle was arrested, and an increase in cell population in the Pre-G, autophagic cell death, as well as necrosis and early and late apoptosis, indicate that cell death was induced) and MNU/testosterone-PCa-induced mice in vivo (induced antiproliferative genes, p53, Bax, TNFalpha, caspase 3,9, repressed antiangiogenic and antiapoptotic genes, VEGF and Bcl2, respectively), successfully slowing the growth of tumors and even killing cancer cells, as well as lowering oxidative stress (MDA), improving the functions of the kidneys (urea, creatinine), liver (ALT, AST), and antioxidants (GPx, GPx, GST, CAT, GSH, TAC). SXPDT, the X-ray photo- or sono-chemical RRBP activation mechanism, and the antioxidant capacity of non-activated RRBP can all be linked to this process.

Conclusion
On the bases of the findings, EP@RRBP-CNP shows a great promise as a novel, efficient selective delivery system for localized SXPDT-activated prostate cancer treatment.
Benchmarking cell type annotation methods for 10x Xenium spatial transcriptomics data
Authors: Cheng Jinming , Jin Xinyi , Smyth Gordon K. , Chen Yunshun 
Published: January 2025

Abstract

Background
Imaging-based spatial transcriptomics technologies allow us to explore spatial gene expression profiles at the cellular level. Cell type annotation of imaging-based spatial data is challenging due to the small gene panel, but it is a crucial step for downstream analyses. Many good reference-based cell type annotation tools have been developed for single-cell RNA sequencing and sequencing-based spatial transcriptomics data. However, the performance of the reference-based cell type annotation tools on imaging-based spatial transcriptomics data has not been well studied yet.

Results
We compared performance of five reference-based methods (SingleR, Azimuth, RCTD, scPred and scmapCell) with the marker-gene-based manual annotation method on an imaging-based Xenium data of human breast cancer. A practical workflow has been demonstrated for preparing a high-quality single-cell RNA reference, evaluating the accuracy, and estimating the running time for reference-based cell type annotation tools.

Conclusions

SingleR was the best performing reference-based cell type annotation tool for the Xenium platform, being fast, accurate and easy to use, with results closely matching those of manual annotation.
Study protocol: multi-centre, randomised controlled clinical trial exploring stromal targeting in locally advanced pancreatic cancer; STARPAC2
Authors: Kocher Hemant M. ,   BCI-STARPAC2 team, Ackermann Charlotte , Priyadarshini Garima , Lawrence Cheryl , Kotriwala Nishtha , Georgescu Ramona ,   BPTB team, Roberts Rhiannon , Hughes Christine , Imrali Ahmet , Chelala Claude ,   Precision-Panc team, Dixon-Hughes Judith , Chang David K. , Sasieni Peter , Corrie Pippa , McNamara Mairéad G. , Sarker Debashis , Froeling Fieke E. M. , Christie Alan , Gillmore Roopinder , Khan Khurum , Propper David 
Published: January 2025

Abstract

Background
Pancreatic cancer (PDAC: pancreatic ductal adenocarcinoma, the commonest form), a lethal disease, is best treated with surgical excision but is feasible in less than a fifth of patients. Around a third of patients presentlocally advanced, inoperable, non-metastatic (laPDAC), whose stadrd of care is palliative chemotherapy; a small minority are down-sized sufficiently to enable surgical excision. We propose a phase II clinical trial to test whether a combination of standard chemotherapy (gemcitabine & nab-Paclitaxel: GEM-NABP) and repurposing All Trans Retinoic Acid (ATRA) to target the stroma may extend progression-free survival and enable successful surgical resection for patients with laPDAC, since data from phase IB clinical trial demonstrate safety of GEM-NABP-ATRA combination to patients with advanced PDAC with potential therapeutic benefit.

Methods
Patients with laPDAC will receive at least six cycles of GEM-NABP with 1:1 randomisation to receive this with or without ATRA to assess response, until progression or intolerance. Those with stable/responding disease may undergo surgical resection. Primary endpoint is progression free survival (PFS) defined as the time from the date of randomisation to the date of first documented tumour progression (response evaluation criteria in solid tumours [RECIST] v1.1) or death from any cause, whichever occurs first. Secondary endpoints include objective response rate (ORR), overall survival (OS), safety and tolerability, surgical resection rate, R0 surgical resection rate and patient reported outcome measures (PROMS) as measured by questionnaire EQ-5D-5L. Exploratory endpoints include a decrease or increase in CA19-9 and serum Vitamin A over time correlated with ORR, PFS, and OS.

Discussion
STARPAC2 aims to assess the role of stromal targeting in laPDAC.

Trial registration
EudraCT: 2019–004231-23; NCT04241276; ISRCTN11503604.
Enhanced spatial analysis assessing the association between PFAS-contaminated water and cancer incidence: rationale, study design, and methods
Authors: Jones Resa M. , Kulick Erin R. , Snead Ryan , Wilson Robin Taylor , Hughes John , Lillys Ted 
Published: January 2025

Abstract

Background
Cancer is a complex set of diseases, and many have decades-long lag times between possible exposure and diagnosis. Environmental exposures, such as per- and poly-fluoroalkyl substances (PFAS) and area-level risk factors (e.g., socioeconomic variables), vary for people over time and space. Evidence suggests PFAS exposure is associated with several cancers; however, studies to date have various limitations. Few studies have used rigorous spatiotemporal approaches, and, to our knowledge, none have assessed cumulative exposures given residential histories or incorporated chemical mixture modeling. Thus, spatiotemporal analysis using advanced statistical approaches, accounting for spatially structured and unstructured heterogeneity in risk, can be a highly informative strategy for addressing the potential health effects of PFAS exposure.

Methods
Using population-based incident cancer cases and cancer-free controls in a 12-county area of southeastern Pennsylvania, we will apply Bayesian spatiotemporal analysis methods using historically reconstructed PFAS-contaminated water exposure given residential histories, and other potential cancer determinants over time. Bayesian group index models enable assessment of various mixtures of highly correlated PFAS chemical exposures incorporating mobility/residential history, and contextual factors to determine the association of PFAS-related exposures and cancer incidence.

Discussion
The purpose of this paper is to describe the Enhanced PFAS Spatial Analysis study rationale, study design, and methods.
Experiences of change following a blended intervention for adults with ADHD and emotion dysregulation: a qualitative interview study
Authors: Nordby Emilie S. , Schønning Viktor , Barnes Alice , Denyer Hayley , Kuntsi Jonna , Lundervold Astri J. , Guribye Frode 
Published: January 2025

Abstract

Background
Emotion dysregulation commonly co-occurs with attention-deficit hyperactivity disorder (ADHD), leading to a range of negative outcomes. While psychological interventions have shown promise in bringing about positive changes in emotional and cognitive domains, there is still limited knowledge on the subjective experiences of change among the participants in these interventions.

Aim
The present study explores the experiences of adults with ADHD who had participated in a blended digital and face-to-face intervention aimed at improving emotion dysregulation. The study focuses on understanding their experiences of change and identifying contributors to change.

Methods
A total of 9 adults with ADHD participated in individual semi-structured, in-depth interviews following their participation in the intervention. The interviews were analyzed using thematic analysis.

Results
The thematic analysis resulted in three core themes. The first theme, ‘perceiving change’, represents changes that the participants experienced, with four subthemes: 1a) being in control, 1b) feeling aware, 1c) accepting oneself and one’s emotions and 1d) gaining insight and knowledge. The second theme, ‘supporting change’, captures factors that supported the participants’ changes, with five subthemes: 2a) acquiring skills, 2b) being in it together, 2c) therapist guidance, 2d) finding motivation 2e) putting it into practice. Lastly, the third theme, ‘sustaining change’, includes aspects important to maintain change, with two subthemes: 3a) working consistently and 3b) giving it time.

Conclusions
The findings show that the participants experienced various changes related to the management of their emotions following their participation in the intervention. Change was perceived as a multifaceted process, supported by internal factors such as motivation and engagement, along with external factors such as support from the other group members and the therapists. Taken together, the findings from the study could be important to the development of psychological interventions for adults with ADHD and may provide valuable knowledge to clinicians and policymakers.

Trial registration
Registered on 21st November 2022 at ClinicalTrials.gov. ClinicalTrials.gov ID: NCT05644028.
Evaluating access to psychosocial services for the medicaid-insured children in Georgia
Authors: Xie Yujia , Harati Pravara , Rajbhandari-Thapa Janani , Serban Nicoleta 
Published: January 2025

Abstract

Background
Evaluating access to psychosocial services can inform policy decision-making on ways to address shortages in the availability of mental health (MH)-specialized providers. The objective of the study was to assess how the mental health (MH)-specialized workforce met the demand for psychosocial services of Medicaid-insured children in Georgia, with direct relevance in establishing quantitative network adequacy.

Methods
We used the 2018 Medicaid (TAF) claims data, the 2018 National Plan and Provider Enumeration System database, and the 2019 Georgia school-based program data to estimate community-level demand and practice-level supply of psychosocial services. We evaluated the availability of services using mathematical models. The outcome measures were met demand and travel distance. We explored the impact of increasing in-home care delivery, expanding Medicaid participation, or increasing caseload for the Medicaid-participating providers on improving met demand for psychosocial services.

Results
Our findings showed that 34% of the demand from Medicaid-insured children in Georgia remained unmet, and 25% of the Georgia census tracts (rural 79%; urban 16%) had < 50% service coverage. The travel distance for in-clinic services was 3.84 miles on average. Increasing provider Medicaid caseload or expanding Medicaid participation, resulting in a 5–40% supply increase, would reduce unmet demand to 7% and decrease the number of unserved and underserved census tracts to 3% and 2% respectively. Meeting 75% of the demand required a 15% increase in the supply.

Conclusions
The main source of network inadequacy was the scarcity of MH providers available to Medicaid-insured children in Georgia, coming from both the limited caseload of existing MH providers and low Medicaid participation, rather than travel constraints. Increasing provider caseload and expanding Medicaid participation were found to reduce unmet demand. Interventions increasing caseloads were the most effective intervention since existing Medicaid-participating providers already had sufficient network coverage geographically.
Evaluating the synergy: anxiety prevalence and alcohol consumption patterns in high-income countries using Granger causality analysis
Authors: Senarathne Binguni , Palliyaguru Dinithi , Oshini Anne , Gamage Janudi , Jayathilaka Ruwan , Rajamanthri Lochana , Wickramarachchi Colinie 
Published: January 2025

Abstract

Background
Alcohol consumption frequently experiences episodes of severe anxiety. This study set out to explore the long-term effects of alcohol consumption on anxiety, revealing insights into how alcohol consumption uniquely impact anxiety, aiming to inform mental health and public health approaches. This research paper explores the complex relationship between the prevalence of anxiety and the consumption patterns of wine, beer, and spirits across fifty-two high-income countries with a continental analysis.

Method
By employing significant secondary data taken from the World Health Organization and Our World in Data online databases and covering the period from 1990 to 2019, this study seeks to explore various causality relationships over this period. Its primary objective is to fill the empirical gap in existing research by using Granger causality analysis to reveal the dynamic relationships between the consumption of alcohol and the prevalence of anxiety. This study endeavours to provide a continental analysis of the high-income countries, which allows for including a comprehensive viewpoint in the context of a single investigation.

Results
The findings demonstrate a variety of patterns of causality in alcohol consumption and anxiety prevalence in a one-way causal relationship across specific continents, a bidirectional relationship in others, and no apparent causal relationship in several countries.

Conclusion
The inference made from the study’s results goes beyond scholarly curiosity; it establishes the foundation for further investigations and the development of customised policies aimed at reducing the mutually reinforcing dangers of alcohol consumption and anxiety disorders.
Referring psychiatric patients to occupational health services for earlier return to work – a qualitative implementation study of barriers and facilitators
Authors: Henriksson Mikko , Tikka Christina , Juvonen-Posti Pirjo , Virtanen Marianna , Oksanen Tuula 
Published: January 2025

Abstract

Background
Mental disorders are a major public health challenge, and their prevalence is globally increasing. They substantially affect work ability, quality of life, and the number of years of disability. A new model for referring psychiatric patients to occupational health services (OHS) aims to improve the continuity of care and to promote the early return to work (RTW) of workers with diagnosed mental health conditions. The purpose of this qualitative implementation study was to identify the facilitators of and barriers to implementing the new model.

Methods
We used the Quality Implementation Framework and the Consolidated Framework for Implementation Research (CFIR) as theoretical frameworks. We interviewed the developers of the model and the psychiatrists and occupational health physicians who deliver it. We invited forty participants to join the study, 17 of whom consented. We conducted nine semi-structured group and individual interviews. Data analysis consisted of analysing the sessions, systematically coding the transcribed texts according to the main domains of CFIR, thematic analysis, and identifying the overarching themes and context-related mechanisms.

Results
We identified three overarching themes crucially related to the implementation of the model: uncertainty about the scope and boundaries of the cooperation in the model, ambiguity about the size of the target group, and the existing sociocultural and self-stigma related to mental illness. Shared belief in the importance and the positive effects of the model and trust in the developers were the main facilitators of the implementation of the model. The main barriers were the limited availability of the e-referral system between the psychiatrists and OHS, uncertainty regarding the number of eligible patients, and the low number of actual referrals during implementation.

Conclusion
Collaborative models in mental health care should accommodate various stakeholders from different sectors involved in the treatment and rehabilitation of workers with diagnosed mental health conditions. Helping health care workers contact possible cooperation partners and knowing how to address important individual, workplace-related and sociocultural factors such as stigma may strengthen collaboration between different sectors and stakeholders in mental health care. Future studies should focus on the multi-actor feasibility of the new collaborative models and include the patients’ perspective.