bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dacarbazine-encapsulated solid lipid nanoparticles for skin cancer: physical characterization, stability, in-vivo activity, histopathology, and immunohistochemistry
Authors: Bhattacharya Sankha , Sharma Satyam 
Published: April 2023

BackgroundThis study examined the use of solid lipid nanoparticles (SLNs) to administer Dacarbazine (DTIC) to skin melanoma cells with minimal adverse effects. Melanoma is a tricky skin cancer to cure, and standard chemotherapy has many negative effects. Encapsulating DTIC in SLNs may allow the drug to target melanoma cells without harming healthy cells. The study developed and tested DTIC-loaded SLNs for skin melanoma treatment.MethodsThis study encapsulated Dacarbazine (DTIC) in solid lipid nanoparticles (SLNs). SLNs with reversed micelles were produced utilizing specified ratios of the surfactant Kolliphor® P188 and phosphatidylcholine. To track SLN drug localisation, gold nanoparticles were conjugated to the DTIC. Nanoparticle size and form were examined using DLS and TEM. These approaches ensured SLNs had the correct size and shape for drug delivery.Significant findingsIn the study, various parameters of the developed solid lipid nanoparticles (SLNs) were evaluated, including particle size, zeta potential, polydispersity index (PDI), entrapment efficacy, and cumulative drug permeation. The values for these parameters varied across the different formulations, with particle size ranging from 146 ± 4.71 nm to 715 ± 7.36 nm, zeta potential from -12.45 ± 2.78 mV to -30.78 ± 2.83 mV, PDI from 0.17 ± 0.013 to 0.51 ± 0.023, entrapment efficacy from 37.78 ± 2.78% to 87.45 ± 4.78%, and cumulative drug permeation from 117 ± 4.77 μg/cm2 to 275 ± 5.67 μg/cm2. To determine the optimal anti-cancer formulation, the DTIC-SLNs-8 nanoparticles were mixed with an optimized concentration of Gellan gum (0.01% w/v) and applied to DMBA-induced skin tumors in rats for six weeks, twice daily. Histopathology demonstrated that DTIC-SLNs-8-treated rats had less keratosis, inflammatory responses, and angiogenesis than free DTIC-treated rats. The development of SLNs may be a promising approach for melanoma treatment due to their improved drug retention over the skin. The optimised anti-cancer formulation DTIC-SLNs-8 showed improved efficacy with minimal side effects as compared to free DTIC.
Dexmedetomidine ameliorates liver injury and maintains liver function in patients with hepatocellular carcinoma after hepatectomy: a retrospective cohort study with propensity score matching
Authors: Wang Xiaoqiang , Li Yi-ran , Shi Yumiao , Li Xiaoying , Luo Jiamei , Zhang Yiqi , Qi Bo , Wu Feixiang , Sun Yuming , Pan Zhiying , Tian Jie 
Published: April 2023

BackgroundAlthough dexmedetomidine (DEX) is widely used during the perioperative period in patients with hepatocellular carcinoma (HCC), its clinical effects on liver function and postoperative inflammation are unclear. This study aimed to explore effects of DEX on postoperative liver function and inflammation in patients with HCC after hepatectomy.MethodsA retrospective cohort study with propensity score matching was performed. A total of 494 patients who underwent hepatectomy from June 2019 to July 2020 and fulfilled the eligibility criteria were included in this study. Baseline data, liver function indexes and inflammation-related biomarkers were collected and compared between the two groups. Survival analysis was conducted to investigate the effects of DEX on the overall survival (OS) of patients. Propensity score matching (PSM) was used to minimize bias between the two groups.ResultsThe study cohort comprised 189 patients in the DEX-free group and 305 patients in the DEX group. Patients in the DEX group had lower levels of alanine transaminase (ALT, P = 0.018) and lactate dehydrogenase (LDH, P = 0.046) and higher level of serum albumin (ALB, P &lt; 0.001) than patients in the DEX-free group before discharge. A total of 107 pairs of patients were successfully matched by PSM. Results consistently suggested that ALT and LDH levels were significantly lower (P = 0.044 and P = 0.046, respectively) and ALB levels were significantly higher (P = 0.002) in the DEX group than in the DEX-free group in the early postoperative period. No significant differences of inflammation-related biomarkers were observed between two groups after PSM. Neither the Kaplan–Meier survival analysis nor the multiple Cox regression survival analysis identified DEX as a contributing factor that would affect the OS of patients after PSM.ConclusionDEX exerts protective effects on liver function while has little effects on inflammation-related biomarkers in the early postoperative period in patients undergoing hepatectomy due to HCC.
Baseline neutrophil-to- ratio combined with the change during treatment provides risk stratification for metastatic malignant melanoma patients treated with PD-1 inhibitors in a Chinese population
Authors: Wang Chen , Liu Shengyan , Li Xin , Cui Kang , Zhang Weijie , Du Yabing 
Published: April 2023

BackgroundPrevious studies have suggested that an elevated baseline neutrophil-to-lymphocyte ratio (BLNLR) and elevated relative change of NLR (ΔNLR%) is associated with worse outcomes in patients with a variety of cancers. This study aims to investigate the value of BLNLR and ΔNLR% before the third cycle of treatment on the prognosis of patients with metastatic malignant melanoma treated with PD-1 inhibitors.MethodsA total of 63 patients with metastatic malignant melanoma treated with PD-1 inhibitors in the First Affiliated Hospital of Zhengzhou University from January 2017 to December 2021 were retrospectively analyzed. BLNLR and ΔNLR% before the third cycle of treatment were collected. The Kaplan-Meier method was used to draw survival curves and Log-Rank test was used for survival analysis. Univariate and multivariate Cox regression analysis were used to analyze the relationship between BLNLR, ΔNLR% and clinical characteristics with progression-free survival (PFS) and overall survival (OS).ResultsUnivariate analysis showed that PFS and OS were associated with BLNLR, ΔNLR%, BMI and number of metastatic organs (P &lt; 0.05). Multivariate analysis showed that BLNLR, ΔNLR%, BMI and number of metastatic organs were independent predictors of OS and BLNLR and ΔNLR% were independent predictors of PFS. Patients were divided into four groups according to BLNLR (&lt;3, ≥3) and ΔNLR% (&lt; 30%, ≥30%): low-BLNLR + low-ΔNLR% group, low-BLNLR + high-ΔNLR% group, high-BLNLR + low-ΔNLR% group, high-BLNLR + high-ΔNLR% group. The median OS was 20 months, 8 months, 9 months, 5 months and the median PFS was 8 months, 3 months, 2 months, 2 months, respectively.ConclusionBLNLR combined with ΔNLR% can be used to predict the prognosis of PD-1 inhibitors in patients with metastatic malignant melanoma.
De novo 11q13.3q13.4 deletion in a patient with Fanconi renotubular syndrome and intellectual disability: Case report and review of literature
Authors: Shen Yingxiao , Xu Xiaoqin , Chen Jiansong , Wang Jingjing , Dong Guanping , Huang Ke , Fu Junfen , Wu Dingwen , Wu Wei 
Published: April 2023

ObjectiveTo explore the genetic etiology of a child with facial dysmorphia, developmental delay, intellectual disability, Fanconi renotubular syndrome, and Chiari malformations.Materials and methodsWhole exome sequencing (WES), Copy number variation sequencing (CNV-seq), and mitochondrial gene detection (Long-PCR + NGS) were applied to detect possible pathogenic mutations and chromosomal copy number variations (CNVs), together with databases and literature reviews to clarify the pathological significance of the candidate mutations.ResultsThe WES revealed a 2.10 Mb interstitial deletion from 11q13.3 to 11q13.4, which was later confirmed by CNV-seq involving 11 OMIM genes, among which SHANK2, DHCR7, NADSYN1, FADD, NUMA1, IL18BP, ANO1, and FGF3 are disease-causing. The mitochondrial gene shows no variations.ConclusionThe child has carried a de novo 11q13.3q13.4 microdeletion, in which SHANK2 genes may be the key gene responsible for the phenotype of intellectual disability. The renal manifestation of the child, which can be diagnosed as Fanconi renotubular syndrome, has an unknown cause but may result from the effect of the ANO1 gene. This case adds a new phenotype to the deletion of this region.
Microfiber optomechanical torsion sensor
Authors: Zhang Qiang , Zhang Jie , Yang Shiwei , Zhai Ruili , Xie Yuyang , Li Yongmin 
Published: April 2023

In this paper, we propose and demonstrate experimentally an optomechanical torsion sensor using a microfiber mechanical resonator. The torsion angle could be obtained by monitoring the resonant frequency shifts of the microfiber resonator. Theoretical and experimental results show that the shift of resonant frequency is non-linear to the torsion angle, and the fundamental mode is more sensitive than other higher modes. The highest sensitivity of the sensor tested in our experiments is 1,687 Hz/degree, and the corresponding resolution of torsion angle is up to 0.0006°, which is 2 orders of magnitude higher than that of the reported fiber-optic torsion sensors. The proposed sensor is a promising candidate for the practical engineering applications.
Methodological considerations for determining the volume and intensity of drop jump training. A systematic, critical and prepositive review
Authors: Montoro-Bombú Raynier , Sarmento Hugo , Buzzichelli Carlo , Moura Nelio Alfano , Gonzáles Badillo Juan José , Santos Amândio , Rama Luís 
Published: April 2023

This systematic review provides critical and propositional information on criteria for determining the volume and intensity of drop jumps during plyometric training programs. Eligibility criteria were defined according to PICOS: Participants: male or female athletes, trained or recreationally active (16–40 years). Intervention duration: more than 4 weeks. Comparators: passive or active control group during a plyometric training program. Outcomes: information on improvement with Drop Jump or Depth Jump, with other jumps, acceleration, sprint, strength, and power output. Design: randomized controlled trials. We searched articles published in PubMed, SPORTDiscus, Web of Science, and Scopus. The search was conducted until 10 September 2022 for English-language articles only. The risk of bias was assessed using Grading of Recommendations, Assessment, Development and Evaluation (GRADE) for randomized controlled studies. We identified 31495 studies, of which only 22 were included. We found that six groups presented results with women, 15 presented results with men, and the remaining four presented mixed studies. Of the 686 people recruited, 329 participants aged 25.79 ± 4.76 years were involved in training. Methodological problems in training intensity, volume distribution, and individualization were noted, but methodological recommendations for their solution are also provided. It is concluded that the drop height should not be understood as the intensity determinant of plyometric training. Intensity is determined by ground reaction forces, power output, and jump height, among other factors. Furthermore, the athletes’ experience level selection should be based on the formulas recommended within this research. These results could be helpful for those who intend to conduct new plyometric training programs and research.
Mandarin Chinese L1 and L2 complex sentence reading reveals a consistent electrophysiological pattern of highly interactive syntactic and semantic processing: An ERP study
Authors: Chen Luyao , Yang Mingchuan , Gao Fei , Fang Zhengyuan , Wang Peng , Feng Liping 
Published: April 2023

IntroductionA hallmark of the human language faculty is processing complex hierarchical syntactic structures across languages. However, for Mandarin Chinese, a language typically dependent on semantic combinations and free of morphosyntactic information, the relationship between syntactic and semantic processing during Chinese complex sentence reading is unclear. From the neuropsychological perspective of bilingual studies, whether second language (L2) learners can develop a consistent pattern of target language (i.e., L2) comprehension regarding the interplay of syntactic and semantic processing, especially when their first language (L1) and L2 are typologically distinct, remains to be determined. In this study, Chinese complex sentences with center-embedded relative clauses were generated. By utilizing the high-time-resolution technique of event-related potentials (ERPs), this study aimed to investigate the processing relationships between syntactic and semantic information during Chinese complex sentence reading in both Chinese L1 speakers and highly proficient L2 learners from South Korea.MethodsNormal, semantically violated (SEM), and double-violated (containing both semantic and syntactic violations, SEM + SYN) conditions were set with regard to the nonadjacent dependencies of the Chinese complex sentence, and participants were required to judge whether the sentences they read were acceptable.ResultsThe ERP results showed that sentences with “SEM + SYN” did not elicit early left anterior negativity (ELAN), a component assumed to signal initial syntactic processing, but evoked larger components in the N400 and P600 windows than those of the “SEM” condition, thus exhibiting a biphasic waveform pattern consistent for both groups and in line with previous studies using simpler Chinese syntactic structures. The only difference between the L1 and L2 groups was that L2 learners presented later latencies of the corresponding ERP components.DiscussionTaken together, these results do not support the temporal and functional priorities of syntactic processing as identified in morphologically rich languages (e.g., German) and converge on the notion that even for Chinese complex sentence reading, syntactic and semantic processing are highly interactive. This is consistent across L1 speakers and high-proficiency L2 learners with typologically different language backgrounds.
Online group music therapy: proactive management of undergraduate students’ stress and anxiety
Authors: Finnerty Rachael , McWeeny Sean , Trainor Laurel 
Published: April 2023

IntroductionIn alignment with the World Health Organization’s (WHO) goal to provide comprehensive and integrated mental health services in community-based settings, this randomized control trial explored the efficacy of online group music therapy as a proactive intervention for reducing stress and anxiety in university students who do not necessarily have a diagnosis.MethodsThe study took place during COVID-19 restrictions. Students who volunteered were randomly assigned to 6 weeks of weekly (1) online active group music therapy, (2) online receptive group music therapy, (3) online group verbal therapy (standard of care), or (4) no-intervention (control group). Students rated their stress (Likert scale) and anxiety [State-Trait Anxiety Inventory, State version (STAI-S)], and provided heart rate variability (HRV) using a phone app, pre and post each therapy session.ResultsSTAI-S and Likert stress scores significantly reduced from pre to post 45-min online music therapy sessions, with moderate evidence that these changes did not differ from the standard of care (verbal therapy). HRV results were not analyzed statistically as HRV collection was likely compromised due to challenges of remote collection. Students completed the Perceived Stress Scale (PSS) and provided a hair sample for cortisol analysis before and after the 6-week intervention. Changes in stress from week 1 to week 6 were not observed in the PSS measure; however, cortisol increased significantly in the control group as the term progressed, while it remained relatively stable in the therapy groups, suggesting therapy may lead to greater control of stress. Of participants’ demographic characteristics, music sophistication, personality, and changes in quality of life, only the personality trait of conscientiousness correlated significantly with PSS, suggesting online group therapy may be beneficial for a wide range of university students.DiscussionThe results suggest group music therapy can be as effective as group verbal therapy. Further, the study indicates that online delivery can be achieved effectively, supporting the idea that remote therapy may be a viable option for other populations. While the study should be replicated with a larger multi-site sample, it provides one example toward achieving a health-promoting culture on university campuses, consistent with the mental health goals of the Okanagan Charter.
Therapeutic hypothermia as a neuroprotective strategy in newborns with perinatal asphyxia—case report
Authors: Oliveira Nayara Rodrigues Gomes de , Teixeira Gustavo Gonçalves , Fernandes Kathlen Terezinha Montes Soares , Avelar Marla Moreira , Medeiros Maja , Formiga Cibelle Kayenne Martins Roberto 
Published: April 2023

BackgroundPerinatal asphyxia is a public health problem and the third major cause of death among children under 5 years.ObjectiveTwo clinical cases of newborns with perinatal asphyxia submitted to therapeutic hypothermia and the follow-up of their motor development after hospital discharge have been reported.MethodsThis retrospective case report study included two newborns with hypoxic-ischemic encephalopathy due to perinatal asphyxia who received a hypothermia protocol at the neonatal intensive care unit (NICU). The two newborns and their families were followed up at the outpatient clinic and assessed using the Hammersmith Child Neurological Examination, Alberta Child Motor Scale, and Denver Developmental Screening Test-II.ResultsThe newborns were submitted to a 72-hour hypothermia protocol. One newborn remained for 13 days in the NICU, while the other remained for 22 days. According to the multidisciplinary team, both cases presented with typical motor development with no cerebral palsy during the follow-up.ConclusionBoth cases showed positive results and a good prognostic for motor development. Therapeutic hypothermia may be a strategy to prevent neurologic sequelae in newborns with perinatal asphyxia, including cerebral palsy.
State of the art in subtotal cholecystectomy: An overview
Authors: Ramírez-Giraldo Camilo , Torres-Cuellar Andrés , Van-Londoño Isabella 
Published: April 2023

IntroductionSubtotal cholecystectomy is a type of surgical bail-out procedure indicated when facing difficult laparoscopic cholecystectomy due to not reaching the critical view of safety, inadequate identification of the anatomical structures involved and/or risk of injury.Materials and methodsA comprehensive search on PubMed were performed using the following Mesh terms: Subtotal cholecystectomy and Partial cholecystectomy. The PubMed databases were used to search for English-language reports related to Subtotal cholecystectomy between January 1, 1987, the date of the first published laparoscopic cholecystectomy, through January 2023. 41 studies were included.ResultsSubtotal cholecystectomy's incidence oscillates between 4.00% and 9.38%. Strasberg et al., divided subtotal cholecystectomies in “fenestrating” and “reconstituting” types based on if the remaining portion of the gallbladder was left open or closed. Subtotal cholecystectomy can sometimes be a challenging procedure and is associated to a high rate of complications such as biliary fistula, retained gallstones, subhepatic or subphrenic collections, among others.ConslusionSubtotal cholecystectomy is a safe alternative when facing difficult cholecystectomy in which the critical view of safety is not reached in order to avoid complications. A classification system should be implemented in surgical descriptions to compare the different surgical techniques employed. In order to avoid bile leakage and cholecystitis of the remnant gallbladder, the surgical technique must be performed skillfully. There is still a current lack of information on alternative techniques such as omental plugging or falciform patch in order to judge their utility. There needs to be further research on long-term complications such as malignancy of the remnant gallbladder.
Evaluation of surgical outcomes in elderly patients with rib fractures: A single-centre propensity score matching study
Authors: Zhang Dong , Jiao Chenbo , Xi Siqi , Wang Langran , Li Run , Zhang Qiang 
Published: April 2023

BackgroundRib fractures are the most common injuries in chest trauma. Compared with younger patients, elderly patients with rib fracture have a higher incidence of complications and mortality. A retrospective study was conducted to investigate the effect of internal fixation compared with conservative treatment on the outcome of rib fracture in elderly patients.Material and methodsWe used a 1:1 propensity score matching method to perform a retrospective analysis of 703 elderly patients with rib fractures treated in the Thoracic Surgery Department of Beijing Jishuitan Hospital between 2013 and 2020. After matching, the length of hospital stay, death, symptom relief and rib fracture healing were compared between the surgery and the control group.ResultsThe study included 121 patients receiving SSRF in the surgery group and 121 patients receiving conservative treatment in the control group. The length of hospital stay in the surgery group was significantly longer than that in the conservative group (11.39d vs. 9.48d, p = 0.000). After 9 months of follow-up, the fracture healing rate in the surgery group was significantly higher than that in the control group (96.67% vs. 88.89%, p = 0.020). The fracture healing time (p = 0.000), improvement in pain score (p = 0.000) and duration of pain medication use (p = 0.000) were also significantly better in the surgery group than in the control group.ConclusionCompared with conservative treatment, surgical treatment can prolong hospital stay to some extent. However, it has the advantages of more rapid healing and lessened pain. For rib fractures in elderly individuals, surgical treatment is a safe and effective option under strict surgical indications and is recommended.