bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of novel hybrid 3D-printed degradable artificial joints incorporating electrospun pharmaceutical- and growth factor-loaded nanofibers for small joint reconstruction
Authors: Hsu Yung-Heng , Chou Ying-Chao , Chen Chao-Lin , Yu Yi-Hsun , Lu Chia-Jung , Liu Shih-Jung 
Published: July 2023

Abstract
Background
Small joint reconstruction remains a challenge and can lead to prosthesis-related complications, mainly because of the suboptimal performance of the silicone materials used and adverse host reactions. In this study, we developed hybrid three-dimensionally printed polycaprolactone (PCL) artificial joints and electrospun drug- and biomolecule-loaded poly(lactic-co-glycolic acid) nanofibers for small joint reconstruction.
Methods
The mechanical properties of the degradable joints and the drug discharge patterns of the nanofibers were evaluated. Moreover, he drug discharge patterns in vivo and the efficacy of the hybrid joint/nanofibers were assessed using a rabbit knee joint model.
Results
The empirical data showed that 3D-printed PCL joints exhibited good mechanical and fatigue properties. The drug-eluting nanofibers sustainably released teicoplanin, ceftazidime, and ketorolac in vitro for over 30, 19, and 30 d, respectively. Furthermore, the nanofibers discharged high levels of bone morphogenetic protein-2 and connective tissue growth factors for more than 30 d. An in vivo animal test showed that nanofiber-loaded joints released high concentrations of antibiotics and analgesics in the rabbit model for 28 d. The animals in the drug-loaded degradable joint group showed greater activity counts than those in the surgery-only group.
Conclusions
The experimental data of this study illustrated that degradable joints with sustained discharge of biomolecules may be utilized in small joint arthroplasty.
Clinical and multiparametric MRI features for differentiating uterine carcinosarcoma from endometrioid adenocarcinoma
Authors: Chen Xiaodan , Guo Qingyong , Chen Xiaorong , Zheng Wanjing , Kang Yaqing , cao dairong 
Published: July 2023

Abstract
Introduction The purpose of our study was to differentiate uterine carcinosarcoma (UCS) from endometrioid adenocarcinoma (EAC) by the multiparametric magnetic resonance imaging (MRI) features.
Methods We retrospectively evaluated clinical and MRI findings in 17 patients with UCS and 34 patients with EAC proven by histologically. The following clinical and pathological features were evaluated: age, post- or pre-menopausal, clinical presentation, invasion depth, FIGO stage, lymphaticmetastasis. The following MRI features were evaluated: tumor dimension, cystic degeneration or necrosis, hemorrhage, signalintensity (SI) on T2-weighted images (T2WI), relative SI of lesion to myometrium on T2WI, T1WI, DWI, ADCmax, ADCmin, ADCmean (RSI-T2, RSI-T1, RSI-DWI, RSI-ADCmax, RSI-ADCmin, RSI-ADCmean), ADCmax, ADCmin, ADCmean, the maximum, minimum and mean relative enhancement (RE) of lesion to myometrium on the arterial and venous phases (REAmax, REAmin, REAmean, REVmax, REVmin, REVmean). Receiver operating characteristic (ROC) analysis and the area under the curve (AUC) were used to evaluate prediction ability.
Results The mean age of UCS was higher than EAC. UCS occurred more often in the postmenopausal patients. UCS and EAC did not significantly differ in depth of myometrial invasion, FIGO stage and lymphatic metastasis. The anterior-posterior and transverse dimensions were significantly larger in UCS than EAC. Cystic degeneration or necrosis and hemorrhage were more likely occurred in UCS than EAC. The tumor on T2WI was more heterogeneous in UCS. The RSI-T2, ADCmax, ADCmean, RSI-ADCmax and RSI-ADCmean of UCS were significantly higher than EAC. The REAmax, REAmin, REAmean, REVmax, REVmin and REVmean of UCS were all higher than EAC. The AUCs were 0.72, 0.71, 0.86, 0.96, 0.89, 0.84, 0.73, 0.97, 0.88, 0.94, 0.91, 0.69 and 0.80 for the anterior-posterior, transverse dimensions, RSI-T2, ADCmax, ADCmean, RSI-ADCmax, RSI-ADCmean, REAmax, REAmin, REAmean, REVmax, REVmin and REVmean, respectively. Our study showed that REAmax threshold value of 0.23 can differentiate UCS from EAC with 94% sensitivity, 88% specificity, and 0.97AUC.
Discussion Multiparametric MRI features may be utilized as a biomarker to distinguish UCS from EAC.
Knowledge does not explode but increases linearly over time
Authors: Wang Xinbing , Kang Huquan , Fu Luoyi , Ding Jiaxin , Liang Shiyu , Wang Jianghao , Zhou Lei , Zhou Chenghu 
Published: July 2023

Abstract
ChatGPT and GPT-4 have raised debates regarding the progress of knowledge in large language models 1-3. The notion of "knowledge explosion" has been controversial in various variations since the 19th century 4-8. Despite numerous indications to the contrary 9-11, conclusive evidence on knowledge growth is lacking 12. Here, we evaluated knowledge as a collective thinking structure within citation networks by analyzing large-scale datasets containing 213 million publications (1800–2020) and 7.6 million patents (1976–2020). We found that knowledge did not explode but grew linearly over time in naturally formed citation networks that expanded exponentially. Our theoretical analysis established that the knowledge never exceeds the size of the network, revealing the limitation of knowledge development. Moreover, our results showed that the knowledge expansion rate shifted at certain inflection points, implying quantitative-driven qualitative changes. Leaps near inflection points may instigate a "knowledge explosion" delusion, allowing us to reconcile the spreading of the misconception. Inflection points in knowledge growth exhibited similar characteristics to the emergent ability of artificial intelligence 13, furnishing fresh insights into the singularities and emergence in complex systems. Overall, our findings reveal a slow pace of knowledge compared to data, reacquainting us with the progress of knowledge over time.
EGFR protein editing by serine hydroxymethyl transferase SHMT2
Authors: Chin Y. Eugene , Du Juanjuan , Zeng Yuan-yuan , Li Xue , Huang Fang , Zhou Chuanzan , Zi Rui-dong , Liu Lu-ying , Luo Jianyuan , Zhao Ting 
Published: July 2023

Abstract
The epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase, relies on phosphorylation for signal transduction1,2. It is known that the EGFR gene often harbors activating mutations for protein sequence change, leading to constitutive phosphorylation and activation of the EGFR protein in various cancers3. This study reveals that EGFR proteins, isolated from lung cancer tissues or cell lines, possess multiple glycine-to-serine (G>S) protein sequence modifications, which are not associated with EGFR gene mutations. These EGFR proteins enlist Serine Hydroxymethyl Transferase 2 (SHMT2) to induce glycine hydroxymethylation, resulting in G>S/GS>SG protein sequence editing. SHMT2 catalyzes the protein editing predominantly within the GS-islands of the EGFR protein, utilizing either free serine amino acids present within cells or the serine residue adjacent to glycine residue as the hydroxymethyl group donor. The G>S/GS>SG editing within GS islands G719S720, G810S811, and G1103S1104 causes significantly amplify of EGFR signaling activity, stimulates cancer cell growth, and induces drug resistance. In lung cancer cells, depletion of SHMT2 or inhibition with SHIN1 suppresses glycine hydroxymethylation, eliminating EGFR protein G>S/GS>SG editing and reducing resistance to EGFR-targeting drugs. Our findings not only identify a novel form of protein mutation through directly editing, but also underscore the importance of protein editing driving EGFR activation, thereby presenting potential avenues for EGFR-targeted therapy.
Beyond chronological age: Exploring how different future time perspective influences the health tourism intention
Authors: Liao Caisheng , Xiao Huiqing , Li Zhao 
Published: July 2023

Abstract

Background Health tourism is growing steadily and become an extremely popular component of modern tourism. This study constructed a theoretical model to examine the influential relationships between future time perspective (FTP), health awareness, involvement, and health tourism intention.
Methods This study was conducted among people with normal cognitive function and at least 15 years of age and collected empirical data by means of a questionnaire. The main method of operation was to distribute online questionnaires to collect data on two of the most popular social media (WeChat and QQ) platforms in China. The data was analyzed primarily using SPSS 26.0 and AMOS 26.0.
Results The effect of different dimensions of FTP on health tourism intentions differed. However, limited future time perspective (LFTP) positively influenced health tourism intentions, while open-ended future time perspective (OFTP) did not have a significant effect on health tourism intentions. Involvement and health awareness not only directly influence health tourism intentions, but also mediate the relationship between FTP (OFTP and LFTP) and health tourism intentions respectively.
Conclusions The results show that This study revealed the mechanism of the role of factors other than chronological age and health tourism intentions, suggesting that health tourism companies should pay more attention to subjective factors other than chronological age. According to the findings, the theoretical and managerial implications of the study are discussed, and directions for future research are provided.
A collision tumor of nasopharyngeal carcinoma and primary mantle cell lymphoma in the nasopharynx: A case report and review of the literature
Authors: Jiang Meng , Yuan Xiao-ping , Zhang Hong , Li Chuang-quan , Mao Yong-lin , Chen Wei-liang 
Published: July 2023

Abstract
Background
Nasopharyngeal carcinoma (NPC) is more common in men aged 40 to 59 years, and radiotherapy is an effective treatment. Nasopharyngeal lymphoma (NPL) is rare, and the coexistence of mantle cell lymphoma (MCL) and NPC in the nasopharynx is even rarer. No reports to date have described a collision tumor of NPC and MCL occurring within the same nasopharyngeal mass. We herein report the successful treatment of a unique case of synchronous coexistence of NPC and MCL occurring in the nasopharynx of a Chinese man.
Case presentation:
A 58-year-old man presented with a 5-month history of swallowing discomfort. Biopsy was taken under nasopharyngoscopy and histopathology revealed NPC. Lesions of the nasopharynx, oropharynx and tonsils, and enlargement lymph nodes of parotid gland, retroauricular and cervical regions were found by magnetic resonance imaging (MRI), which may be a synchronous, double primary tumor in which NPC and NPL co-exist. Pathology consultation confirmed that the biopsy specimen of nasopharynx was a collision tumor of NPC and MCL. Positron emission tomography–computed tomography (PET-CT) revealed thickening of the posterior wall of the nasopharynx, and NPC with lymphoma was considered. Enlargement of pharyngeal lymphatic ring and multiple lymph nodes with high metabolism were evaluated as lymphoma infiltration. The patient received two courses of R-CHOP chemotherapy (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone) followed by head and neck radiotherapy. At the time of this writing, he had remained alive without recurrence for 61 months since the initial treatment and was still undergoing follow-up.
Conclusions
Magnetic resonance imaging helps identify different components of collision tumors. Pathological examination helps to confirm the diagnosis. Histological examination reveals different components and PET-CT can help determine the extent of the lesion. Dose-adjusted chemotherapy combined with radiotherapy may have good therapeutic effects, but additional case studies are needed for confirmation.
Preparation and properties of nanocellulose- reinforced environmentally adaptable PAM/PVA semi- interpenetrating highly conductive ion-conducting hydrogel
Authors: Huang Xinmin , Wang Yaning , Tan Xiaobin , Yang Lianhe 
Published: July 2023

Abstract
In this work, a semi-interpenetrating network (IPN) hydrogel reinforced with cellulose nanocrystals (CNC) and containing lithium chloride (LiCl) and dimethyl sulfoxide (DMSO) is composed of cross-linked polyacrylamide (PAM) and linear polyethylene alcohol (PVA) chains, which had excellent anti-drying, anti-freezing, and ionic conductivity performance. The experimental results show that the ion-conducting hydrogel has excellent cyclic stretching performance. Under the tensile strain of 200%, the relative resistance of the PAM/PVA/CNC/LiCl hydrogel remained unchanged after 10 stretching cycles and returned to its original length. The water retention rate of PAM/PVA/CNC/LiCl hydrogel is close to 80% at 60 °C. In addition, even at -20 °C, the hydrogel still has good electrical conductivity and exhibits excellent anti-freezing performance. The ion-conducting hydrogel has excellent conductivity and anti-freezing and anti-drying properties and has potential application prospects in flexible wearable devices and sensors.
STK11 loss leads to YAP1-mediated transcriptional activation in human KRAS-driven lung adenocarcinoma cell lines
Authors: Seward David , Lenahan Sean , Sarausky Hailey , Deming Paula 
Published: July 2023

Abstract
Serine Threonine Kinase 11 (STK11) loss of function (LoF) correlates with anti-PD-1 therapy resistance in patients with KRAS-driven lung adenocarcinoma (LUAD). The molecular mechanisms governing this observation remain unclear and represent a critical outstanding question in the field of lung oncology. As an initial approach to understand this phenomenon, we knocked out STK11 in multiple KRAS-driven, STK11-competent human LUAD cell lines and performed whole transcriptome analyses to identify STK11-loss-dependent differential gene expression. Subsequent pathway enrichment studies highlighted activation of the HIPPO/YAP1 signaling axis, along with the induction of numerous tumor-intrinsic cytokines. To validate that YAP1-mediated transcriptional activation occurs in response to STK11 loss, we pursued YAP1 perturbation as a strategy to restore an STK11-competent gene expression profile in STK11-KO LUAD cell lines. Together, our data link STK11loss with YAP1-mediated transcriptional activation, including the upregulation of immune-evasion promoting cytokines IL-6, CXCL8 and CXCL2. Further, our results raise the intriguing possibility that YAP1 antagonism may represent a therapeutic approach to counter anti-PD-1 therapy resistance in STK11-null, KRAS-driven LUADs by modulating tumor-intrinsic gene expression to promote a “hot” tumor immune microenvironment.
FimH of uropathogenic Escherichia coli influenced the infection in prostate cells by the modulation of JAK/STAT signaling pathway
Authors: Ho Chen-Hsun , Huang Tzu-Wen , Fan Chia-Kwung , Liu Shih-Ping , Cheng Po-Ching 
Published: July 2023

Abstract

Background: The FimH expression may be a key factor affecting prostatitis caused by UPEC infection. Whether its regulation by the JAK/STAT pathway increases resistance to inflammation caused by infection with high FimH-expressing UPEC strains requires investigation.
Methods: The effect of FimH on the ability of knockout ΔFimH UPEC, FimHover -expressed UPEC, and wild-type strains to invade prostate cells and induce inflammation and the effects of different FimH levels on testosterone-treated UPEC and regulation of the JAK/STAT pathway were analyzed.
Results: Comparison of the three strains revealed the inhibitory effects of testosterone were more significant in the ΔFimH strain. Testosterone-pretreated ΔFimH UPEC showed weak inflammatory responses and JAK/STAT expression. FimHover UPEC better resisted the inhibitory effects of testosterone, which there was no significantly decreases except 20 μg/ml pretreated group in most JAK/STAT-related proteins. The effects of FimH showed a concentration-dependent response to testosterone, particularly to JAK1, STAT3, and pSTAT3, which also affected the subsequent expression of TLR4, IL-6, and IFN-γ. It has been suggested that regulation of the JAK1/STAT3 pathway may be associated with the effects of the FimH virulence factor on the inhibition of testosterone in UPEC infection.
Conclusion: The inhibitory effect of testosterone on UPEC infection in prostate epithelial cells was affected by the virulence factor FimH of UPEC, and reduced the production of inflammatory factors. The JAK/STAT pathway plays a key role in regulating UPEC infection and influences testosterone suppression responses in prostate cells. Our study provides a possible guideline for using testosterone to treat clinical recurrent UPEC infection and persistent prostatitis.
Skeletal muscle status and survival among patients with advanced biliary tract cancer
Authors: Takaoka Shinya , Hamada Tsuyoshi , Takahara Naminatsu , Saito Kei , Endo Go , Hakuta Ryunosuke , Ishida Kota , Ishigaki Kazunaga , Kanai Sachiko , Kurihara Kohei , Oyama Hiroki , Saito Tomotaka , Sato Tatsuya , Suzuki Tatsunori , Suzuki Yukari , Tange Shuichi , Tokito Yurie , Tateishi Ryosuke , Nakai Yousuke , Fujishiro Mitsuhiro 
Published: July 2023

Abstract
Background
Studies have demonstrated a prognostic role of sarcopenia (i.e., loss of skeletal muscle volume and functionality) in patients with various cancer types. In patients with biliary tract cancer, the quantity and quality of skeletal muscles and their serial changes have not been fully investigated in relation to survival outcomes.
Methods
We identified 386 patients with unresectable or recurrent biliary tract cancer and calculated skeletal muscle index (SMI) and skeletal muscle density (SMD) to estimate muscular quantity and quality, respectively, based on computed tomography images. Using the Cox regression model with adjustment for potential confounders, we calculated hazard ratios (HRs) and 95% confidence intervals (CIs) for progression-free survival (PFS) and overall survival (OS) according to skeletal muscle status and its serial change.
Results
Compared to patients without sarcopenia, patients with sarcopenia had multivariable HRs of 1.56 (95% CI, 1.13–2.17; P = 0.008) and 1.23 (95% CI, 0.99–1.53; P = 0.059) for PFS and OS, respectively. SMD at baseline was associated with OS with a multivariable HR comparing the extreme quartiles of 1.54 (95% CI, 1.09–2.18; Ptrend = 0.010). A reduction in SMI rather than that in SMD was associated with OS. Progressive disease was a risk factor for reductions in SMI and SMD.
Conclusions
Skeletal muscle quantity and quality and their serial changes were associated with survival outcomes in patients with advanced biliary tract cancer. Our data highlight the importance of designing nutritional and physical interventions for improvements in skeletal muscle status in this population.
The relationship between COVID-19 event strength and job burnout among primary health care staff: The chain mediating effect of job stress and work engagement
Authors: Li Xinru , Song Yiwen , Hu Bingqin , Chen Yitong , Cui Peiyao , Liang Yifang , He Xin , Li Jinghua 
Published: July 2023

Abstract
Background
As a global pandemic, The Corona Virus Disease 2019 (COVID-19) has brought significant challenges to the primary health care (PHC) system. Health professionals are constantly affected by the pandemic's harmful impact on their mental health and are at significant risk of job burnout. Therefore, it is essential to gain a comprehensive understanding of how their burnout was affected. The study aimed to examine the relationship between COVID-19 event strength and job burnout among PHC providers and to explore the single mediating effect of job stress and work engagement and the chain mediating effect of these two variables on this relationship.
Methods
We used multilevel stratified convenience sampling to recruit participants from PHC institutions in Jilin Province, China. A total of 1148 medical professionals completed questionnaires regarding sociodemographic characteristics, COVID-19 event strength, job stress, work engagement, and job burnout. The chain mediation model was analysed using SPSS PROCESS 3.5 Macro Model 6.
Results
COVID-19 event strength not only positively predicted job burnout, but also indirectly influenced job burnout through the mediation of job stress and work engagement, thereby influencing job burnout through the "job stress → work engagement" chain.
Conclusions
This study extends the application of event systems theory and enriches the literature about how the COVID-19 pandemic impacted PHC medical staff job burnout. The findings derived from our study have critical implications for current and future emergency response and public policy in the long-term COVID-19 disease management period.
Subgroups of depressive Symptoms determined by a Latent Class Analysis in a Chinese College Students Population during COVID-19
Authors: Luo Cong , Li Jiahong , Liu Lili , Huang Andi , Wang Xiayong , Chen Rongning , Zhao Jingbo 
Published: July 2023

Abstract
Background: The ongoing epidemic continues to exert a lasting impact on the physical and mental health of students. Therefore, it is crucial to identify the related risk factors and target populations with a high risk of mental health issues.
Method: A sample of 6309 participants from 6 colleges in Guangdong Province, China was recruited from June 10 to 18, 2021. Depressive symptoms were assessed using the Patient Health Questionnaire and risk factors were assessed using the Intolerance of Uncertainty (IU) scale and demographics. Data analysis consisted of chi-square test, latent class analysis, and Multinomial Logistic Regression.
Result: (1) Four latent subgroups were identified: “low-symptom group (2244, 35.57%),” “moderate-symptom group (1323, 20.97%),” “major-symptom with low-risk of suicide group (1128, 17.88%),” and “major-symptom with high-risk of suicide group (1614, 25.58%).” (2) Students with high IU were more likely to report severe suicidal ideation (OR=14.14, p<0.001,). (3) Although females were more likely to have depressive symptoms (OR=1.55, p<0.001,), but that of male may be server once they had depressive symptoms (OR=0.73, p<0.001,). (4) The students with severe depressive symptoms were likely to be non-exercise, uninfected area, unvaccinated and severer impact on family economy
Conclusion: Our findings offer valuable data support for identifying subgroups that are particularly susceptible to developing depressive symptoms during the epidemic. These results have important implications for mental health interventions and the formulation of public health policies.