bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-methyladenosine-modified PIAS1 promotes microglia polarization to M1 by regulating PPARγ SUMOylation in Inflammatory Bowel Disease-associated hyperalgesia
Authors: Huang Yulin , Xu Rui , Hou Bailing , Sun Yu’e , Mao Yanting , Yang Yan , Gu Xiaoping , Zhang Wei , Ma Zhengliang 
Published: September 2023

Abstract

Background and Aims: Large number of nociceptive signals produced by intestinal inflammation are transmitted to the spinal dorsal horn, leading to microglia polarization, which is of great significance in mediating central sensitization. Emerging evidence indicate that m6A modification regulates a dynamic macrophage polarization. However, it remains unsolved to link polarization of microglia regulated by m6a modification to inflammatory bowel disease (IBD) associated hyperalgesia. Methods IBD-induced hyperalgesia was assessed by paw withdrawal mechanical threshold in a murine colitis model induced by dextran sulphate sodium (DSS). MeRIP sequence was performed on the LPS-treated primary microglia. The biological role and mechanism of m6A modified PIAS1 in microglia polarization and central sensitization were determinedin vitroandin vivo. Results The level of m6A RNA was significantly increased in the spinal dorsal cord of colitis mice, accompanied by microglia polarization to M1-proinflammatory phenotype, and METTL3 was the main regulator involved in the abundant m6A RNA modification. Moreover, MeRIP sequence identified PIAS1 acts anti-inflammatory function though promoting PPARγ SUMOylation in regulating microglia polarization. Mechanistically, the m6A reader YTHDF2 directly recognized and bound to the m6A site on PIAS1 mRNA and reduced PIAS1 mRNA stability. Overexpression YTHDF2 decreased PIAS1 mRNA level and promoted the polarization to M1-phenotypein vitro. Finally, reduced m6A modification by specific downregulation METTL3 in microglia of the spinal dorsal horn contributes to alleviate IBD-associated hyperalgesia. Conclusions The mRNA stabilization of m6A-modified PIAS1-mediated PPARγ SUMOylation as the underlying mechanism through which METTL3 preserved M1-phenotype microglia contributes to IBD-associated hyperalgesia.
Revelation of inherent trends in Mucormycosis resurgence in India: A comparative analysis of past prevalence (pre-Covid-19) and current outbreaks
Authors: Pant Pratibha , Pant Prashant , Rajpal Vijay Rani , Singh Apekshita , Arya Harshit , Sonkar Anand , Chandra Atika , Raina Soom Nath 
Published: September 2023

Abstract
Mucormycosis (MM), commonly referred to as ‘Black Fungus’ was a relatively lesser-known fungal infection until the onset of Covid-19 pandemic. However, amidst the global Covid-19 outbreak, it emerged as a widespread fungal infection causing significant morbidity and mortality. In India, the recorded incidence of MM was approximately 80% higher than in the rest of the world due to a higher prevalence of specific pre-disposing factors, causal organisms, clinical manifestations, and intriguing epidemiological trends. This study compared the MM case-control studies conducted in India before the Covid-19 pandemic and during the current pandemic to comprehend the impact of Covid-19 on the surge in MM cases. Our findings demonstrate that MM is a distinct condition which is not solely dependent on Covid-19. Interestingly, the trends of association of MM with comorbidities like diabetes and its greater prevalence in male gender remains consistent in both study periods. The increased occurrence of MM in India during the current pandemic appears to be more intricately linked to challenges in management and treatment of Covid-19, leading to emergence of novel predisposing factors. The indiscriminate use of steroids, immunosuppressants, and the resultant hyperglycemic condition, especially in a population already burdened with diabetes as comorbidity contributed significantly to the current MM havoc. The study suggests that raising general awareness about preventive measures, diabetes management and the regulation of steroid drug misuse can play a crucial role in curtailing the development and spread of deadly infections like MM in future.
Ginsenoside rb2 regulates tissue autophagy in the ischemic penumbra area and delays BMSC senescence by improving the ischemic-hypoxic microenvironment
Authors: WANG SHIQI , Hui Xiao-shan , Zhang Hao , Zhang Hui , Zhang Chen-ming , Zhang Jin-sheng , Xie Fei 
Published: September 2023

Abstract
Introduction: Ischemic cardiovascular disease (CVD), is associated with high morbidity and mortality. Panax notoginseng (PNS) has some advantages in improving ischemic cardiovascular diseases, but the mechanism of action of its active ingredient ginsenoside Rb2 is not clear.
Methods: In this study, network pharmacology, molecular docking, and LC-MS/MS were used to evaluate the relationship between PNS and its main active constituent and autophagy. In vivo and in vitro investigations were then used to further confirm the results.
Results: In this investigation, we discovered that the primary active component of PNS, ginsenoside Rb2, might increase tissue autophagy in myocardial infarction rat models' ischemic semidark zone. In the cell model, ginsenoside Rb2 boosted the proliferation rate and decreased the apoptosis rate of bone marrow mesenchymal stem cells (BMSCs). And alleviated oxidative stress, delayed aging, raised GATA4mRNA, lowered cTNTmRNA levels, and protected BMSCs from the harm brought on by ischemic-hypoxic injury.
Discussion: Our research initially established an ischemic-hypoxic cell model, which may be provided as a reference for related research. And clarified the functions of ginsenoside Rb2 in the ischemic-hypoxic microenvironment, which may be conducive to the exploration of therapeutic targets of ischemic CVD.
Improvement in methane concentration in the biogas produced through the co-digestion of waste from the seafood restaurant industry and mezcal vinasses.
Authors: Sagrero Kathia Amital Lemus , Carmen Chávez-Parga Ma. Del , Cortés José Apolinar 
Published: September 2023

Abstract
The release of waste by the mezcal and seafood restaurant industries, due to their characteristics, generates adverse effects on soils, bodies of water, and air. An important aspect of anaerobic digestion is having an inoculum whose microbial consortium is in suitable conditions for carrying out the anaerobic process in its four stages. Applying the combined pretreatments (chemical, thermal, and volumetric ratio of waste) modifies its bioavailability and, consequently, its consumption rate by the microorganisms in the anaerobic inoculum. This research focused on evaluating the strategy of having an enriched inoculum, co-digestion of waste, and applying pretreatments to methane production in the biogas produced through the BMP test. The development of the tests was carried out considering a Taguchi L4 orthogonal experimental design for the variables in two levels of pretreatment: by temperature, volumetric ratio of waste from the restaurant industry of seafood waste and mezcal vinasses, and chemical by pH adjustment, for an initial concentration of residue mixture of 18 g VS L-1; the monitoring variables were biogas production, methane concentration, volatile solid, pH, conductivity, and alkalinity. The results show that the enriched inoculum used in the test had a pH of 8.18, a conductivity of 19.24 mS cm-1, an alkalinity of 31 g CaCO3 L-1, and an alpha (α) ratio of 0.75; the time in which the BMP test was carried out was of the order of 29 days. At the best test, with pretreatment at a temperature of 50°C, a residue ratio of 1 VSW/ 2 VMV, and a pH of 6.5, a production of 1420.51 ± 0.038 N mL CH4 and an 82.3 % CH4 concentration in the biogas were reached, with a yield of 175.37 N mL CH4 g-1 VS and a degradation constant of 0.14 d-1.
Role of NLRP3 inflammasomes in monocyte and microglial recruitments in choroidal neovascularization
Authors: Dieckmann Blake W. , Paguaga Marcell E. , McCollum Gary W. , Penn John S. , Uddin Imam 
Published: September 2023

Abstract

Though the pathogenesis of choroidal neovascularization (CNV) is largely unknown in age-related macular degeneration (AMD), inflammasomes may contribute to CNV development and progression. To understand the role NLRP3 inflammasomes in CNV, we used Ccr2RFPCx3cr1GFP dual-reporter mice to characterize migration of Ccr2RFP positive monocytes and Cx3cr1GFP positive microglial cells into CNV lesions after laser-induced rupture of Bruch’s membrane. MCC950 was used as NLRP3 inhibitor. Immunostaining was used to confirm localization of NLRP3 inflammasomes in the LCNV lesions. Confocal microscopy was used to image and quantify LCNV volumes. ELISA and qRT-PCR were used to confirm the activation of NLRP3 by monitoring the expression of IL-1β protein and mRNA in choroidal tissues from LCNV mice. In addition, NLRP3 (-/-) LCNV mice were used to investigate whether NLRP3 inflammasomes contribute to the development of LCNV lesions. We observed that RFP positive monocyte-derived macrophages and GFP positive microglia-derived macrophages, in addition to other cell types, were localized in LCNV lesions at day 7 post-laser injury. In addition, NLRP3 inflammasomes are associated with LCNV lesions. Inhibition of NLRP3 inflammasomes, using MCC950, caused an increased Ccr2RFP positive macrophages, Cx3cr1GFP positive microglia, and other cells resulting in an increase in total lesion size. NLRP3 (-/-) LCNV mice, showed significantly increased lesion size compared to age-matched controls. Inhibition of NLRP3, resulted in decreased IL-1β mRNA and protein expression in the choroidal tissues, suggesting that increased lesion size may not be directly related to IL-1β.
The anxiety caused by long-term low-dose ketamine, and related metabolomics characteristics change in rat
Authors: Fu Yingqiang , Zou Jing , Zhao Wensong , Yang Hong , Xiao Li , Sun Yiming , Jiang Zihan , Lin Yao , Ye Yi , Ma Chunling , Liao Linchuan 
Published: September 2023

Abstract
Ketamine is a kind of dissociation anesthesia, clinical studies have demonstrated the ability of ketamine to induce rapid and sustained antidepressant actions. However, considering the side effects such as dissociative properties, psychotomimetic effect, neurotoxicity, and abuse potential, the safety profile of prolonged use of ketamine still needs to be investigated. To investigate the effects of repeated administration of low-dose ketamine on locomotor activity, and mood changes in rats. To explore the metabolome and metabolic pathway change in plasma, and prefrontal cortex of rats after chronic administration and withdrawal of low-dose ketamine.To provide evidence for toxicity studies of ketamine as an antidepressant. Rat’s behavior changes induced by ketamine administration and withdrawal were observed by elevated plus-maze experiments. Untargeted metabolomics analysis of rat plasma and prefrontal cortex tissues was performed by UHPLC-QE/MS to screen differential expression metabolites and explore differential metabolic pathways. The number of entries into (0.80 ± 1.17 times, p < 0.05) and duration in (12.48 ± 13.65s, p < 0.01) open-arms were significantly lower than that of the control group (3.40 ± 1.62 times, 59.74 ± 23.32s), showing stronger anxiety degree. After withdrawal, the anxiety of rats was relieved. 74 differential metabolites were screened in the plasma of ketamine group, involving cysteine and methionine metabolism, valine and isoleucine biosynthesis, glutamine and glutamate metabolism. After one week of withdrawal, the cysteine and methionine metabolic pathways were still significantly different with the saline group; 87 metabolites in the prefrontal cortex significantly changed, which involved purine metabolism, ascorbic acid, and aldose metabolism pathways.
Arthrospira maxima hydrolysate as a high value biofertilizer in basil (Ocimum basilicum L.) seedlings
Authors: Marín-Marín Camila Andrea , Estrada José Alberto , Naranjo Juan Martín Delgado , Ocampo Paola Andrea Zapata 
Published: September 2023

Abstract
Continued increase of human populations and use of chemical fertilizers remain a threat to the health and stability of human-ecological systems worldwide. To ameliorate this problem and achieve sustainable agriculture, a variety of ecofriendly technologies have been developed, including the production of cyanobacteria-based biofertilizers. This technology can be optimized through experiments that assess how plant growth is enhanced under different biofertilizer concentrations (g L− 1). Here we assess the biofertilizer potential of hydrolysates derived from the cyanobacteria Arthospira maxima on the growth of basil (Ocimum basilicum) under six different concentrations (0, 2.5, 5, 10, 20, and 40 g L− 1), and an additional treatment where we applied the registered product Optimar ® (4 mL L− 1). For all response variables except stem length, a concentration of 40 g L− 1 yielded significantly higher values. The beneficial effects of the application of Optimar were often surpassed when plants received cyanobacteria concentrations above 10 g L− 1. A chemical composition analysis of A. maxima revealed high concentrations of the phytohormones gibberellic acid (0.19 g L− 1), 6-benzyladenine (0.37 g L− 1), and 1-naphthaleneacetic acid (0.25 g L− 1) that are likely responsible for the cyanobacteria’s biostimulant activity. Further research is required to determine how other crop plants respond to different concentrations of A. maxima and the viability of creating an economically accessible product with a higher concentration of A. maxima using circular economy and biorefinery concepts to obtain high value-added by-products.
Uric acid, high density lipoprotein cholesterol and their ratio are related to microbial enterotypes and serum metabolites in females with blood stasis constitution
Authors: Sun Chen , Liu Yanmin , Huang Wei , Chen Yang , Deng Yusheng , Yuan Jiaming , Deng Lili , Xu Ning , Shang Xiaoxiao , Wang Chuyang , Yang Zhimin , Huang Li , Qiu Qinwei 
Published: September 2023

Abstract
Background
Blood stasis constitution in Traditional Chinese Medicine (TCM) is believed to render individuals more susceptible to metabolic diseases. However, the biological underpinnings of this constitutional imbalance remain unclear.
Methods
This study explored the association between blood stasis constitution, serum metabolic markers including uric acid (UA), high-density lipoprotein cholesterol (HDLC) and their ratio (UHR), gut microbiota and serum metabolites. Clinical data, fecal and serum samples were collected from 24 individuals with blood stasis constitution and 80 with balanced constitution among healthy subjects from Guangdong. Gut microbiota composition analysis and serum metabolomics analysis were performed.
Results
Females with blood stasis constitution had higher UA levels, lower HDLC levels, and higher UHR in serum, suggesting a higher risk of metabolic abnormalities. Analysis of the gut microbiome revealed two distinct enterotypes dominated by Bacteroides or Prevotella. Intriguingly, blood stasis subjects were disproportionately clustered within the Bacteroides-rich enterotype. Metabolomics analysis identified subtle alterations between groups, including lower phenylalanine and higher trimethylaminoacetone levels in blood stasis. Several differential metabolites displayed correlations with HDLC, UA, or UHR, unveiling potential new markers of metabolic dysregulation.
Conclusions
Our findings elucidate the intricate interplay between host constitution, gut microbiota, and serum metabolites. The concept of blood stasis offers a unique perspective to identify subtle alterations in microbiome composition and metabolic pathways, potentially signaling underlying metabolic vulnerability, even in the presence of ostensibly healthy profiles. Continued investigation of this TCM principle may reveal critical insights into the early biological processes that foreshadow metabolic deterioration.
Effect of Air Annealing on Structural, Textural, Thermal, Magnetic and Photocatalytic Properties of Ag-Doped Mesoporous Amorphous Crystalline Nanopowders Bi2O3
Authors: Ilves V. G. , Gaviko V. S. , Murzakaev A. M. , Sokovnin S. Y. , Svetlova O. A. , Zuev M. G. , Uimin M.A. 
Published: September 2023

Abstract
Ag doped Bi2O3 nanopowders (NPs) were produced by pulsed electron beam evaporation (PEBE) under vacuum. The solid phase synthesis in an electric furnace on air was used for silver doping of bismuth oxide (addition of - 1 and 5 wt% crystalline silver nitrate). Structural, textural, thermal and magnetic properties of Ag doped Bi2O3 NPs have been studied. The specific surface area of (SSA) Ag- Bi2O3 NPs was 23.7 m2/g, which was almost 2 times bigger than the SSA of the pure Bi2O3 (13.2 m2/g). Air annealing (200 °C) caused decreased crystallinity and growth of SSA in all NPs, without exception. Silver doping reduced the grain size of NPs after they were air annealed compared to the grain size of NPs annealed pure bismuth oxide. The dominant phase in not annealed/annealed Ag doped Bi2O3 NPs at 200 °C and 300 °C was β -phase Bi2O3 with a tetragonal structure. Accordingly, the photocatalytic activity of the Ag doped NPs was higher in the doped Bi2O3. The thermal stability of the pure and Ag-doped Bi2O3 NPs was maintained at 300-350 °C. The phase transition β→α occurred with a further increase in temperature. The annealing temperature could effectively change the physicochemical properties of the Bi2O3 NPs.
Cellulose/graphene nanoplatelets cryogel for adsorption of dyes in an aqueous medium
Authors: Lazzari Lídia K. , Zattera Ademir J. , Santana Ruth M. C. 
Published: September 2023

Abstract
The development of new technologies for the removal of dyes present in industrial effluents has been the subject of great interest in recent years, due to the increase in awareness about environmental preservation and the greater rigor of specific legislation. In this context, adsorption is one of the most promising techniques for color removal, since it has a relatively low cost, in addition to having appreciable efficiency. The use of graphene nanoplatelets (GNP) as a reinforcement for cellulose cryogels brings interesting properties to it, such as increased mechanical strength and adsorption capacity (oils, dyes...). Considering the mentioned aspects, this work aims to evaluate the adsorbent capacity of cellulose/GNP cryogel in the removal of congo red organic dye in an aqueous medium. The differential of the present work will be the use of GNP as a carbonaceous structure to reinforce the cellulose cryogel. Thus, a potential material will be developed for application as an adsorbent in the removal of dyes in an aqueous medium.
Spatio-temporal changes and hydrological forces of wetland landscape pattern in the Yellow River Delta during 1986-2022
Authors: Qiu Mengqi , Liu Yanxu , Chen Peng , He Naijie , Wang Shuai , Fu Bojie 
Published: September 2023

Abstract
Context:
Estuarine wetlands provide valuable ecosystem services but have undergone continuous change under the pressures of climate change and anthropogenic disturbance. The Yellow River Delta (YRD) wetland, which is one of the most vigorous delta areas in the world, has undergone dramatic changes. However, the long-term and high-resolution typical salt marsh vegetation evolution processes and hydrological drivers remain unclear.
Objectives
(1) Generate annual mapping of salt marsh vegetation in the YRD wetland from 1986 to 2022. (2) Analyze the trends of wetland patch area and landscape pattern, and explain the hydrological drivers of landscape pattern evolution.
Methods
Combining Landsat 5‒8 and Sentinel-2 images, vegetation phenology, remote sensing indices, and Random Forest supervised classification to map the typical salt marsh vegetation. We applied piecewise linear regression model to analyze YRD wetland changes, and stepwise multiple linear regression was used for assessing the impact of hydrological factors on landscape pattern.
Results
We identified three stages of landscape pattern evolution with 1997 and 2009 as turning points, including the rapid expansion stage, gradual decline stage, and bioinvasion stage. In the first phase, the wetland area was expanded by 70.45%, while the typical salt marsh vegetation, Phragmites australis area was reduced by 25%. In the second phase, the wetland was reduced by 21.33% and the Phragmites australis area was reduced by 15.96%. The third stage, Spartina alterniflora demonstrated an unstoppable trend of rapid expansion, with an area increase of 68 times relative to 2009, expanding at an average rate of 344 hm2 per year.
Conclusions
Areas of wetland, tidal flat, and Phragmites australis are significantly influenced by cumulative sediment and cumulative runoff, which total explain 61.5%, 75.7% and 63.8% of their variations, respectively. Wetland and tidal flat areas increased with the cumulative sediment, while cumulative runoff had a weak negative effect. As for Phragmites australis, cumulative runoff had a positive effect, whereas cumulative sediment had a negative effect. Water resources regulation measures should be taken to prevent the degradation of wetland ecosystems, and intervention measures can be implemented during the seedling stage to control the invasion of Spartina alterniflora.
BDNF Val66Met moderates increases in CSF t-tau and p-tau181 in early sporadic Alzheimer’s disease: A prospective cohort study
Authors: Thomson Diny , Rosenich Emily , Maruff Paul , Lim Yen Ying 
Published: September 2023

Abstract
Background: Allelic variation in the brain-derived neurotrophic factor (BDNF) Val66Met polymorphism has been shown to moderate rates of cognitive decline in preclinical sporadic Alzheimer’s disease (i.e., Aβ+ older adults), and pre-symptomatic autosomal dominant AD (ADAD). In ADAD, Met66 was also associated with greater increases in CSF levels of total-tau (t-tau) and phosphorylated tau (p-tau181). This study sought to determine the extent to which BDNF Val66Met is associated with changes in episodic memory and CSF t-tau and p-tau181 in Aβ+ older adults in early-stage sporadic AD.
Methods: Aβ+ Met66 carriers (n=94) and Val66 homozygotes (n=192) enrolled in the Alzheimer’s Disease Neuroimaging Initiative who did not meet criteria for AD dementia, and with at least one follow-up neuropsychological and CSF assessment, were included. A series of linear mixed models were conducted to investigate changes in each outcome over 10 years, covarying for CSF Aβ42 (except when examining changes in CSF Aβ42), APOE ε4 status, sex, age, baseline diagnosis and years of education.
Results: Aβ+ Met66 carriers demonstrated significantly faster memory decline (d=0.33; 95% CI [0.08, 0.58]) and significantly greater increases in CSF t-tau (d=0.30; 95% CI [0.05, 0.55]) and p-tau181 (d=0.29; 95% CI [0.04, 0.54]) compared to Val66 homozygotes, despite showing equivalent rates of change in CSF Aβ42.
Conclusions: These findings suggest that reduced neurotrophic support, which is associated with Met66 carriage, may increase vulnerability to Aβ-related tau hyperphosphorylation, neuronal dysfunction and cognitive decline in the early stages of sporadic AD. Additionally, these findings highlight the need for clinicopathological models of AD to account for neurotrophic factors and the genes which moderate their expression.