bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-acting recombinant human follicle-stimulating hormone (SAFA-FSH) enhances spermatogenesis
Authors: Kim Daham , Lee Soohyun , Cho Yoon Hee , Kang Min Jeong , Ku Cheol Ryong , Chi Hyunjin , Ahn Jungsuk , Lee Kyungsun , Han Jaekyu , Chi Susan , Song Moo Young , Cha Sang-Hoon , Lee Eun Jig 
Published: February 2023

IntroductionAdministration of follicle-stimulating hormone (FSH) has been recommended to stimulate spermatogenesis in infertile men with hypogonadotropic hypogonadism, whose sperm counts do not respond to human chorionic gonadotropin alone. However, FSH has a short serum half-life requiring frequent administration to maintain its therapeutic efficacy. To improve its pharmacokinetic properties, we developed a unique albumin-binder technology, termed “anti-serum albumin Fab-associated” (SAFA) technology. We tested the feasibility of applying SAFA technology to create long-acting FSH as a therapeutic candidate for patients with hypogonadotropic hypogonadism.MethodsSAFA-FSH was produced using a Chinese hamster ovary expression system. To confirm the biological function, the production of cyclic AMP and phosphorylation of ERK and CREB were measured in TM4-FSHR cells. The effect of gonadotropin-releasing hormone agonists on spermatogenesis in a hypogonadal rat model was investigated.ResultsIn in vitro experiments, SAFA-FSH treatment increased the production of cyclic AMP and increased the phosphorylation of ERK and CREB in a dose-dependent manner. In animal experiments, sperm production was not restored by human chorionic gonadotropin treatment alone, but was restored after additional recombinant FSH treatment thrice per week or once every 5 days. Sperm production was restored even after additional SAFA-FSH treatment at intervals of once every 5 or 10 days.DiscussionLong-acting FSH with bioactivity was successfully created using SAFA technology. These data support further development of SAFA-FSH in a clinical setting, potentially representing an important advancement in the treatment of patients with hypogonadotropic hypogonadism.
The coupling relationship between tourism economy and air quality in China: A province-level analysis
Authors: Zhao Shuhong , Li Qin , Kong Yingying , Chen Xi 
Published: February 2023

Introduction: The development of tourism economy depends on air quality to a large extent, and good air quality is conducive to promoting the development of tourism economy. And it is crucial to understand the coupled and coordinated relationship between them. However, few studies have explored the coupling relationship between tourism and air quality. Therefore, it is necessary to further study the coupling relationship between tourism and air quality.Methods: In this study, an index system was constructed to evaluate the development status of tourism economy and air quality system, and the entropy value method was used to weight the indexes. Using panel data of Chinese provinces from 2014 to 2020, a coupled coordination model was established, focusing on the degree of coordination between tourism economy and air quality.Results: The empirical results show that during the sample period, China’s tourism economic index is decreasing, and the regional development is unbalanced, showing the gradient of eastern region &gt; central region &gt; western region. The air quality index, on the other hand, fluctuates and increases, showing a spatial distribution pattern of “weekly high, high and low” of western region &gt; eastern region &gt; central region. The coupling coordination level of both regions shows an increasing trend, with the eastern region having the highest coupling coordination level and always in the stage of barely balanced development; the central region has the second highest coupling coordination level and the coupling coordination level is steadily increasing; the western region has the worst coupling coordination level and the coupling system tends to decline. The main type of coupling coordination effect is tourism economy lagged type, and the overall performance shows that the ambient air quality condition is better than the tourism economy development.Discussion: This study clearly reveals the evolution, degree and types of coupling coordination in the tourism economy and air quality of the two systems, which contributes to the understanding of coupling and synergy of industrial development and ecological environment. This research also expands the application field of the CCDM model, and has a certain methodology contribution for further exploring the synergy and symbiosis mechanism of tourism economy development and environmental change.
Variations of arbuscular mycorrhizal fungi following succession stages in a tropical lowland rainforest ecosystem of South China
Authors: Yang Huai , Mai Siwei , Liu Wenjie , Fu Jialin , Yang Qiu , Zhang Bin , Huang Biao 
Published: February 2023

IntroductionThe grasslands in the Nature Reserve of Ganshenling, in the south of Hainan Island, were first formed after deforestation disturbance before a natural restoration of shrubs and secondary forests. However, the stages of grassland and shrubs in some parts of Ganshenling regions could not be naturally restored to secondary forests. In addition, the forest form of the secondary forest after 40 years (40a) of succession was similar to that of the secondary forest of 60 years (60a). However, it was not known whether the microorganisms recovered to the level of the secondary forest of 60a. Arbuscular mycorrhizal fungi (AMF) are plant root symbionts that can improve the nitrogen and phosphorus absorption of plants and play a key role in secondary forest succession. An understanding of the essential role of soil AMF in secondary forest succession of tropical rainforest in Ganshenling regions is still limited.MethodsTherefore, the soil of 0–10 cm was collected with the help of a 5-point sampling method in grassland, shrubs, and second tropical lowland rainforest of 40a and 60a. We studied community changes in AMF with the succession and explored the impacts of soil physicochemical properties on soil AMF.ResultsOur findings were as follows: (1) Different successional stages showed divergent effects on soil AMF communities. (2) After 40a recovery, the alpha-diversity indices of AMF recovered to the level of secondary forest of 60a, but the similarity of soil AMF communities only recovered to 25.3%. (3) Species richness of common species, rare species, and all the species of AMF showed a significantly positive correlation with soil nitrogen. (4) OTU10; OTU6, OTU9, and OTU141; OTU3 and OTU38; and OTU2, OTU15, OTU23, and OTU197 were significantly unique AMF for grasslands, shrubs, and secondary forests of 40a and 60a, respectively. (5) The phylogenetic tree and the heatmap of AMF showed that the OTUs in grasslands and shrubs were in contrast to the OTUs in secondary forests of 40a and 60a.DiscussionWe concluded that the succession of a secondary forest after deforestation disturbance was probably limited by its AMF community.
Multimodal MRI radiomic models to predict genomic mutations in diffuse intrinsic pontine glioma with missing imaging modalities
Authors: Khalid Fahad , Goya-Outi Jessica , Escobar Thibault , Dangouloff-Ros Volodia , Grigis Antoine , Philippe Cathy , Boddaert Nathalie , Grill Jacques , Frouin Vincent , Frouin Frédérique 
Published: February 2023

PurposePredicting H3.1, TP53, and ACVR1 mutations in DIPG could aid in the selection of therapeutic options. The contribution of clinical data and multi-modal MRI were studied for these three predictive tasks. To keep the maximum number of subjects, which is essential for a rare disease, missing data were considered. A multi-modal model was proposed, collecting all available data for each patient, without performing any imputation.MethodsA retrospective cohort of 80 patients with confirmed DIPG and at least one of the four MR modalities (T1w, T1c, T2w, and FLAIR), acquired with two different MR scanners was built. A pipeline including standardization of MR data and extraction of radiomic features within the tumor was applied. The values of radiomic features between the two MR scanners were realigned using the ComBat method. For each prediction task, the most robust features were selected based on a recursive feature elimination with cross-validation. Five different models, one based on clinical data and one per MR modality, were developed using logistic regression classifiers. The prediction of the multi-modal model was defined as the average of all possible prediction results among five for each patient. The performances of the models were compared using a leave-one-out approach.ResultsThe percentage of missing modalities ranged from 6 to 11% across modalities and tasks. The performance of each individual model was dependent on each specific task, with an AUC of the ROC curve ranging from 0.63 to 0.80. The multi-modal model outperformed the clinical model for each prediction tasks, thus demonstrating the added value of MRI. Furthermore, regardless of performance criteria, the multi-modal model came in the first place or second place (very close to first). In the leave-one-out approach, the prediction of H3.1 (resp. ACVR1 and TP53) mutations achieved a balanced accuracy of 87.8% (resp. 82.1 and 78.3%).ConclusionCompared with a single modality approach, the multi-modal model combining multiple MRI modalities and clinical features was the most powerful to predict H3.1, ACVR1, and TP53 mutations and provided prediction, even in the case of missing modality. It could be proposed in the absence of a conclusive biopsy.
Association between pre-stroke sarcopenia risk and stroke-associated infection in older people with acute ischemic stroke
Authors: Song Xiaodong , Chen Xufeng , Bai Jie , Zhang Jun 
Published: February 2023

BackgroundStroke-associated infection (SAI) is a common complication after a stroke. The incidence of infection was higher in people with sarcopenia than in the general population. However, the relationship between pre-stroke sarcopenia risk and SAI in older patients has not been confirmed. This study aimed to investigate the association between pre-stroke sarcopenia risk and SAI in older patients with acute ischemic stroke (AIS).MethodsThis retrospective study was conducted by the Peking University People’s Hospital. We evaluated the pre-stroke sarcopenia risk by applying the SARC-F questionnaire. Multivariate logistic regression was applied to explore the association between pre-stroke sarcopenia risk and SAI.ResultsA total of 1,002 elder patients with AIS (592 men; 72.9 ± 8.6 years) were enrolled in our study. Pre-stroke sarcopenia risk was found in 29.1% of the cohort. The proportion of patients with pre-stroke sarcopenia risk was larger in the SAI group than in the non-SAI group (43.2 vs. 25.3%, p   &lt; 0.001). In multivariate logistic analysis, pre-stroke sarcopenia risk was shown to be independently associated with SAI (OR = 1.454, 95% CI: 1.008–2.097, p = 0.045) after adjusting for potential factors. This association remained consistent across the subgroups based on age, sex, body mass index, smoking status, drinking status, diabetes, hypertension, and dyslipidemia.ConclusionPre-stroke sarcopenia risk was independently associated with SAI in older patients with AIS. Our findings highlight the significance of pre-stroke sarcopenia identification in the prevention and management of SAI in this population.
Effect of larger corneal spherical aberration in improving the near visual acuity of eyes implanted with the TECNIS Symfony
Authors: Wang Dandan , Liu Chunlu , Guan Weichen , Lu Ziyi , Zhao Yinying , Zhao Yune 
Published: February 2023

PurposeTo explore the effect of corneal spherical aberration on the visual acuity and visual quality of eyes implanted with the TECNIS Symfony intraocular lens (IOL).MethodsA total of 43 patients with age-related cataract (60 eyes) undergoing phacoemulsification and TECNIS Symfony IOL implantation were enrolled in this study. The uncorrected distance (UDVA), intermediate (UIVA), near visual acuity (UNVA), corrected distance visual acuity (CDVA), contrast sensitivity, and ocular spherical aberration were recorded 3 months after surgery. Preoperative and postoperative corneal spherical aberration were also measured using the iTrace device. Objective scattering index (OSI), modulation transfer function cut-off frequency (MTF cut-off), and Strehl ratio (SR) were measured by the Optical Quality Analyzing System. Catquest-9SF questionnaire were applied too. Spearman’s correlation analysis was used to evaluate the relationship between spherical aberration and visual quality parameters.ResultsPatients were satisfied with their postoperatively visual quality. And the postoperative logMAR UDVA, UIVA, UNVA, and CDVA was 0.05 ± 0.07, 0.04 ± 0.06, 0.15 ± 0.07, and 0.03 ± 0.05, respectively. The mean preoperative corneal spherical aberration was 0.24 ± 0.10 μm, which is the only factor influencing postoperatively UNVA, and it was negatively correlated with UNVA and glare contrast sensitivity under 18 cpd (cycle/degree, cpd) spatial frequency (r = −0.403, −0.300, −0.360; all P &lt; 0.05). Additionally, the greater the residual spherical aberration of the cornea, the better the near vision after operation. The mean postoperative ocular spherical aberration was −0.03 ± 0.07 μm, it was not correlated with visual acuity, contrast sensitivity, and visual quality (all P &gt; 0.05).ConclusionPreoperative positive spherical aberration can benefit near vision while decrease contrast sensitivities at high spatial frequencies when implanted with the TECNIS Symfony IOL.
Adherent-invasive E. coli – induced specific IgA limits pathobiont localization to the epithelial niche in the gut
Authors: Tanaka Rika , Imai Jin , Tsugawa Hitoshi , Eap Karl Bil , Yazawa Masaki , Kaneko Motoki , Ohno Masashi , Sugihara Kohei , Kitamoto Sho , Nagao-Kitamoto Hiroko , Barnich Nicolas , Matsushima Masashi , Suzuki Takayoshi , Kagawa Tatehiro , Nishizaki Yasuhiro , Suzuki Hidekazu , Kamada Nobuhiko , Hozumi Katsuto 
Published: February 2023

Background and aimAdherent-invasive E. coli (AIEC) has been identified as a pathobiont associated with Crohn’s disease (CD), that prefers to grow in inflammatory conditions. Although the colonization by AIEC is implicated in the progression of the disease and exacerbates inflammation in murine colitis models, the recognition and response of host immunity to AIEC remains elusive.MethodsAntibiotic treated female C57BL/6 mice were inoculated by commensal E. coli and LF82 AIEC strains. Luminal-IgA fractions were prepared from feces and their binding to AIEC and other strains was assessed to confirm specificity. IgA binding to isogenic mutant strains was performed to identify the functional molecules that are recognized by AIEC specific IgA. The effect of IgA on epithelial invasion of LF82 strain was confirmed using in vitro invasion assay and in vivo colonization of the colonic epithelium.ResultsPersistent colonization by AIEC LF82 induced secretion of luminal IgA, while commensal E. coli strain did not. Induced anti-LF82 IgA showed specific binding to other AIEC strains but not to the commensal, non-AIEC E. coli strains. Induced IgA showed decreased binding to LF82 strains with mutated adhesin and outer membrane proteins which are involved in AIEC – epithelial cell interaction. Consistently, LF82-specific IgA limited the adhesion and invasion of LF82 in cultured epithelial cells, which seems to be required for the elimination in the colonic epithelium in mice.ConclusionThese results demonstrate that host immunity selectively recognizes pathobiont E. coli, such as AIEC, and develop specific IgA. The induced IgA specific to pathobiont E. coli, in turn, contributes to preventing the pathobionts from accessing the epithelium.
Sex differences in amygdalohippocampal oscillations and neuronal activation in a rodent anxiety model and in response to infralimbic deep brain stimulation
Authors: Vila-Merkle Hanna , González-Martínez Alicia , Campos-Jiménez Rut , Martínez-Ricós Joana , Teruel-Martí Vicent , Lloret Ana , Blasco-Serra Arantxa , Cervera-Ferri Ana 
Published: February 2023

IntroductionDepression and anxiety are highly comorbid mental disorders with marked sex differences. Both disorders show altered activity in the amygdala, hippocampus, and prefrontal cortex. Infralimbic deep brain stimulation (DBS-IL) has anxiolytic and antidepressant effects, but the underlying mechanisms remain unclear. We aimed to contribute to understanding sex differences in the neurobiology of these disorders.MethodsIn male and female rats, we recorded neural oscillations along the dorsoventral axis of the hippocampus and the amygdala in response to an anxiogenic drug, FG-7142. Following this, we applied DBS-IL.ResultsSurprisingly, in females, the anxiogenic drug failed to induce most of the changes observed in males. We found sex differences in slow, delta, theta, and beta oscillations, and the amygdalo-hippocampal communication in response to FG-7142, with modest changes in females. Females had a more prominent basal gamma, and the drug altered this band only in males. We also analyzed c-Fos expression in both sexes in stress-related structures in response to FG-7142, DBS-IL, and combined interventions. With the anxiogenic drug, females showed reduced expression in the nucleus incertus, amygdala, septohippocampal network, and neocortical levels. In both experiments, the DBS-IL reversed FG-7142-induced effects, with a more substantial effect in males than females.DiscussionHere, we show a reduced response in female rats which contrasts with the higher prevalence of anxiety in women but is consistent with other studies in rodents. Our results open compelling questions about sex differences in the neurobiology of anxiety and depression and their study in animal models.
The reasons for not returning to work and health-related quality of life among young and middle-aged patients with stroke: A cross-sectional study
Authors: Pan Xi , Wang Zhi , Yao Lin , Xu Lan 
Published: February 2023

ObjectivesThis study aimed to explore the reasons and influencing factors for non-return to work (non-RTW) within 1 year among young and middle-aged patients with stroke and to assess their health-related quality of life (HRQoL) at 1 year across different reasons.MethodsThe study was conducted as a telephone-based cross-sectional survey. Seven hundred eighty-nine young and middle-aged patients with stroke aged between 18 and 54 years for men and 18 and 49 years for women in the electronic medical system were included. Data collection included demographic characteristics, socioeconomic status, behavioral habits, history of chronic diseases, work status, reasons for non-RTW, and HRQoL.ResultsOf 789 patients, 435 (55.1%) (mean [SD] age, 47.7 [7.8] years) did not return to work within 1 year after stroke. Among the patients who did not RTW, 58.9% were unable to work, 9.7% retired early, 11.03% became full-time homemakers or were unemployed, and 20.5% were reluctant to work. The disordered multiclass logistic regression model showed that the factors influencing the reasons for non-RTW included age, gender, education, income, health insurance, diabetes comorbidity, ability to perform activities of daily living, and mobility of the right upper extremity. Furthermore, patients who were unable to work had significantly lower HRQoL compared to those who had RTW, followed by those who retired early.ConclusionsMore than half did not RTW within 1 year in our study. The results will help inform future research to identify interventions to promote RTW and improve HRQoL for young and middle-aged patients with stroke.
Rodent behavior following a dural inflammation model with anti-CGRP migraine medication treatment
Authors: Reducha Philip V. , Bömers Jesper P. , Edvinsson Lars , Haanes Kristian A. 
Published: February 2023

BackgroundMigraine is a widespread and prevalent disease with a complex pathophysiology, of which neuroinflammation and increased pain sensitivity have been suggested to be involved. Various studies have investigated the presence of different inflammatory markers in migraineurs and investigated the role of inflammation in inflammatory models with complete Freund's adjuvant (CFA) or inflammatory soup added to the dura mater.ObjectiveThe aim of the current study was to examine whether application of CFA to the dura mater would cause behavioral alterations that are migraine relevant. In addition, we investigated the potential mitigating effects of fremanezumab, a CGRP (calcitonin gene-related peptide) specific antibody, following CFA application.MethodsMale Sprague-Dawley rats were randomly divided into six groups: fresh (n = 7), fresh + carprofen (n = 6), fresh + anti-CGRP (n = 6), sham (n = 7), CFA (n = 16), CFA + anti-CGRP (n = 8). CFA was applied for 15 min on a 3 × 3 mm clearing of the skull exposing the dura mater of male Sprague-Dawley rats. We applied the Light/Dark box and Open Field test, combined with the electronic von Frey test to evaluate outcomes. Finally, we observed CGRP immunoreactivity in the trigeminal ganglion.ResultsNo differences were observed in the Light/Dark box test. The Open Field test detected behavior differences, notably that sham rats spend less time in the central zone, reared less and groomed more than fresh + carprofen rats. The other groups were not significantly different compared to sham rats, indicating that activation of the TGVS is present in sham surgery and cannot be exacerbated by CFA. However, for the allodynia, we observed specific periorbital sensitization, not observed in the sham animals. This could not be mitigated by fremanezumab, although it clearly reduced the amount of CGRP positive fibers.ConclusionCFA surgically administered to the dura causes periorbital allodynia and increases CGRP positive fibers in the trigeminal ganglion. Fremanezumab does not reduce periorbital allodynia even though it reduces CGRP positive fibers in the TG. Further work is needed to investigate whether CFA administered to the dura could be used as a non-CGRP inflammatory migraine model.
Diagnosis, differential diagnosis, and treatment for sudden sensorineural hearing loss: Current otolaryngology practices in China
Authors: Chen Nishan , Karpeta Niki , Ma Xin , Ning Xianhui , Liu Xiaoling , Song Jijun , Jiang Zigang , Ma Xiulan , Liu Xiuli , Zhong Shixun , Sun Qing , Liu Jun , Chen Ganggang , Duan Maoli , Yu Lisheng 
Published: February 2023

IntroductionAlthough sudden sensorineural hearing loss (SSNHL) has been attempted to be understood for 70 years, diagnosis and treatment strategies still have strong heterogeneity worldwide, which are reflected in the guidelines issued by countries and the clinical practice of otolaryngologists.MethodsQuestionnaires were sent to registered otolaryngologists nationwide via an online questionnaire system. We investigated the current views and clinical practices of otolaryngologists in mainland China about the diagnosis, examination, and treatment strategies of SSNHL.ResultsMost otolaryngologists supported diagnostic classification via audiograms. Regional economic situation and hospital grade affected application strategies for differential diagnosis. Regarding corticosteroid therapy, 54.9% of respondents opted to discontinue the drug 5 days after systemic administration. Both intratympanic therapy and post-auricular injections were selected by more than half of the respondents as initial and salvage treatments.DiscussionChinese otolaryngologists exhibit heterogeneity in clinical practices for SSNHL, including distinct approaches to combination therapy and local application of steroids. This study pointed out Chinese doctors' similarities, differences, and unique strategies in diagnosing and treating SSNHL and analyzed the possible reasons to help the world understand the current otolaryngology practices in China.