bluuebunny/crossref_metadata_2025_split huggingface dataset:
CAGE13-3 Cruise Report: Sub-seabed CO2 Storage: Impact on Marine Ecosystems (ECO2) (A 7th Framework Programme EU project) PART I.
Authors: Bünz Stefan 
Published: January 2023

The ECO2 project sets out to assess the risks associated with the storage of CO2 below the seabed. Carbon Capture and Storage (CCS) is regarded as a key technology for the reduction of CO2 emissions from power plants and other sources at the European and international level. The proposed project will assess the likelihood of leakage and impact of leakage on marine ecosystems. As part of the ECO2 project, cruise CAGE13-3 intend to acquire a 3D seismic volume (which will act as monitor along with the 3D cube collected in 2011) from the Snøhvit carbon capture area. In addition, we will repeat some of the lines twice and in reversed direction in order to study the repeatability of the P-Cable acquisition for monitoring purposes. The primary objective of the hydrological and biogeochemical sampling in the Snøhvit area is to study any possible effects of a Carbon Capture and Storage (CCS) site on the water column and on the bottom sediment.
The cruise may be known as: HH_2013_ECO2_I
Prenatal diagnosis of fetuses conceived by assisted reproductive technology by karyotyping and chromosomal microarray analysis
Authors: Guo Huan , Sheng Rui , Zhang Xiu , Jin Xuemei , Gu Wenjing , Liu Ting , Dong Haixin , Jia Ran 
Published: January 2023

Background
Invasive prenatal evaluation by chromosomal microarray analysis (CMA) and karyotyping might represent an important option in pregnant women, but limited reports have applied CMA and karyotyping of fetuses conceived by assisted reproductive technology (ART). This study aimed to examine the value of CMA and karyotyping in prenatal diagnosis after ART.

Methods
This retrospective study included all singleton fetuses conceived by ART from January 2015 to December 2021. Anomalies prenatally diagnosed based on karyotyping and CMA were analyzed. Prevalence rates for various CMA and karyotyping results were stratified based on specific testing indications including isolated—and non-isolated ART groups. The rates of CMA findings with clinical significance (pathogenic/likely pathogenic) and karyotype anomalies were assessed and compared to those of local control individuals with naturally conceived pregnancies and without medical indications.

Results
In total, 224 subjects were assessed by karyotyping and CMA. In the examined patients, chromosomal and karyotype abnormality rates were 3.57% (8/224) and 8.93% (20/224), respectively. This finding indicated a 5.35% (12/224)-incremental rate of abnormal CMA was obtained over karyotype analysis (p = 0.019). The risk of CMA with pathogenic findings for all pregnancies conceived by ART (5.80%, 13/224) was markedly elevated in comparison with the background value obtained in control individuals (1.47%, 9/612; p = 0.001). In addition, risk of CMA with clinically pathogenic results in isolated ART groups was significant higher compared to the background risk reported in the control cohort (p = 0.037).

Conclusions
Prenatal diagnosis including karyotyping and CMA is recommended for fetuses conceived by ART, with or without ultrasound findings.
Comparative transcriptome analysis of Armillaria gallica 012m in response to ethephon treatment
Authors: Yang Haiying , He Kaixiang , Cao Yapu , Li Zhihao , Ji Qiaolin , Sun Jingxian , Li Ganpeng , Chen Xin , Mo Haiying , Du Gang , Li Qingqing 
Published: January 2023

Background
Gastrodia elata, known as a rootless, leafless, achlorophyllous and fully mycoheterotrophic orchid, needs to establish symbionts with particular Armillaria species to acquire nutrition and energy. Previous research findings had approved that ethylene (ET) played an important role in plant-fungi interaction and some receptors of ET had been discovered in microorganisms. However, the molecular mechanisms underlying the role of ET in the interaction between G. elata and Armillaria species remain unknown.

Methods
Exiguous ethephon (ETH) was added to agar and liquid media to observe the morphological features of mycelium and count the biomass respectively. Mycelium cultured in liquid media with exiguous ETH (0.1 ppm, 2.0 ppm, 5.0 ppm) were chosen to perform whole-transcriptome profiling through the RNA-seq technology (Illumina NGS sequencing). The DEGs of growth-related genes and candidate ET receptor domains were predicted on SMART.

Results
ETH-0.1 ppm and ETH-2 ppm could significantly improve the mycelium growth of A. gallica 012m, while ETH-5 ppm inhibited the mycelium growth in both solid and liquid media. The number of up-regulated or down-regulated genes increased along with the concentrations of ETH. The growth of mycelia might benefit from the up-regulated expression of Pyr_redox (Pyridine nucleotide-disulphide oxidoreductase), GAL4 (C6 zinc finger) and HMG (High Mobility Group) genes in the ETH-0.1 ppm and ETH-2 ppm. Therefore, the growth of mycelia might be impaired by the down-regulated expression of ZnF_C2H2 and ribosomal protein S4 proteins in the ETH-5 ppm. Seven ET receptor domains were predicted in A. gallica 012m. Based on cluster analysis and comparative studies of proteins, the putative ETH receptor domains of A. gallica 012m have a higher homologous correlation with fungi.

Conclusions
The responses of A. gallica 012m to ETH had a concentration effect similar to the plants’ responses to ET. Therefore, the number of up-regulated or down-regulated genes are increased along with the concentrations of ETH. Seven ET receptor protein domains were predicted in the genome and transcriptome of A. gallica 012m. We speculate that ETH receptors exist in A. gallica 012m and ethylene might play an important role in the plant-fungi interaction.
Phytochemical and Biological Investigation of the Leaves of Tridax procumbens
Authors: Nandi Tirtha , Nayan Md. Iqbal Hossain , Nisha Maida Huda 
Published: January 2023

Aims: Because of various traditional uses of Tridax procumbens leaves, the authors choose the plant for investigating the numerous biological activities. The main objective for this study is to search antioxidant activity, hypoglycemic activity and antibacterial activities with initial phytochemical investigations of the leaves of Tridax procumbens.
Methodology: The phytochemical purposes were achieved by using two different chromatographic techniques thin layer chromatography (TLC), vacuum liquid chromatography (VLC). DPPH test, glucose uptake activity of yeast cells and agar plate diffusion technique were performed to evaluate the antioxidant capacity, hypoglycemic activity and anti-bacterial properties respectively of different fraction obtained from VLC by using different solvent systems such as dichloromethane, butanol, ethyl acetate and methanol. 31.25 µg/ml, 62.5 µg/ml, 125 µg/ml, 250 µg/ml and 500 µg/ml sample were used from each fraction to investigate antioxidant and hypoglycemic activity where vitamin C tablet and metformin were used as standard respectively. Antibacterial capacity was measured by measuring zone of inhibition resulted by the fractions where ciprofloxacin was taken as reference drug.
Results: Some visible spots were identified in the TLC plate which confirms the presence of different compounds in the fractions obtained from the methanolic extract of the leaves of Tridax procumbens. Butanol and ethyl acetate fractions showed the highest antidiabetic activity. In addition to, ethyl acetate, butanol and methanol fractions exerted more than 80% antioxidant capacity in DPPH assay. Furthermore, butanol fraction gives maximum zone of inhibition up to 21 mm against Staphylocuccos aureas which ensure anti-bacterial properties of the leaves of Tridax procumbens especially against Staphylocuccos aureas and its strains.
Conclusion: Notwithstanding the all the research, the leaves had anti-inflammatory properties, which may have been generated by their antioxidant activity. As a result, if more therapeutic research is done, the separated compounds in those fractions may be employed as future therapeutic strategies.
Cases of Orbital Cellulitis Revealing Neglected Intraocular Foreign Bodies
Authors: Abounaceur A. , Elhaj J. Ait , El-Hamraoui K. , Mchachi A. , Benhmidoune L. , Rachid R. , El-Belhadji M. 
Published: January 2023

Background: Orbital cellulitis is defined as acute inflammatory orbital swelling of infectious origin. Most often secondary to sinusitis. Intraocular foreign bodies neglected because of their small size, or sometimes radio-transparent nature, can be difficult to diagnose even with radiology and be responsible for orbital cellulitis. We demonstrate the diagnostic and therapeutic difficulties.
Patients and Methods: This is a retrospective descriptive study of patients with unilateral orbital cellulitis revealing neglected intraocular foreign bodies, conducted in the Department of Adult Ophthalmology, Hospital August 20, 1953, involving 58 patients, from January 2015 until December 2020.
Results: The average age of the patients was 38.5 years. The most affected age group was between 21 and 30 years with a clear male predominance. A decrease in visual acuity was found in all patients (unilateral blindness 43%) and a cellulitis complicated by a purulent melt (43%). All patients received medical treatment, including intravitreal injections of antibiotics in 71% of cases, and surgical treatment consisting of extraction of the foreign body and immediate evisceration of the eyeball in 25% of cases.
Conclusion: Orbital cellulitis, although mostly secondary to sinusitis, can reveal various etiologies such as intra-orbital foreign bodies that can go unnoticed and be life-threatening and functionally damaging, especially when the diagnosis is made late and management is inappropriate. The presence of an intraocular foreign body must be suspected in all cases of orbital trauma associated with a palpebral wound, even if it is minimal, or in the presence of a clinical aggravation. Any delay in diagnosis and/or treatment can lead to serious complications that can affect the functional and even vital prognosis. The surgical treatment consists of the extraction of the foreign body. The recourse to evisceration in our context unfortunately continues to persist at high rates; because of the delay of consultation and thus of the management.
NOVOS MODELOS DE GESTÃO NA ATENÇÃO PRIMÁRIA À SAÚDE E AS PENOSIDADES DO TRABALHO
Authors: Penha de Oliveira Santos Renato , Chinelli Filippina , Ferreira Fonseca Angélica 
Published: January 2023

O artigo trata da reconfiguração do trabalho na Atenção Primária à Saúde (APS) a partir de mudanças implementadas pelo governo federal desde 2017, que agravam as circunstâncias de precarização do trabalho. Metodologicamente, inspira-se na etnografia de caso ampliado de Michael Burawoy, dialogando com estudos sobre a reestruturação produtiva e a Nova Gestão Pública (NGP), articulados ao conceito de penosidades. Utilizou-se a observação participante e entrevistas semiestruturadas com trabalhadoras e uma equipe de saúde de uma unidade básica do Sistema Único de Saúde (SUS) do Rio de Janeiro. Caracterizam-se como penosidades: exigência constante de mudanças na organização do cotidiano do trabalho e sobrecarga devido à extensa jornada de trabalho, e cobrança pelo alcance de metas a despeito das condições de trabalho. Ressalta-se a necessidade de continuar análises da reconfiguração do trabalho em saúde, considerando os impactos da Emenda Constitucional nº 95 (2016); da reforma trabalhista (2017) e da pandemia de Covid-19.
TRABALHO E RACISMO NA “ARACAJU ROMÂNTICA” DOS ANOS 1940 E 1950
Authors: Gomes José Edwyn Silva 
Published: January 2023

Nos areais da “Aracaju Romântica dos anos 1940 e 1950” histórias de mulheres e homens negros se desenrolaram, verdadeiras tramas foram protagonizadas por estes sujeitos até então “excluídos da história”. Apresentamos resultados preliminares acerca da história de vida de Rosalina Santos. É uma grande honra “costurar” a história de vida da minha prima.
Costureira das “madames” brancas da capital, neta de ex-escravizados, Rosalina Santos nasceu em Divina Pastora/SE em 1924 e se destacou como costureira da dita “alta sociedade aracajuana”. Com resultados preliminares, apresentamos aspectos do seu cotidiano, identificando nas suas memórias o racismo presente nas relações de trabalho. Quais foram os efeitos do racismo cotidiano na sua trajetória? Como foi possível tornar-se costureira dessa clientela? Quais eram as condições de trabalho para mulheres negras nesse período? Quais foram as suas estratégias, suas tomadas de decisão?
The partograph in the management of labor following cesarean section
Authors: Khan K.S. , Rizvi A. 
Published: July 2002

AbstractObjective: To determine whether graphic labor record (partogram) can be used to predict the risk of uterine scar rupture in labor following lower segment cesarean section. Methods: Between 1988 and 1991, 236 women had a trial of labor following cesarean section. After the onset of the active phase (3 cm cervical dilatation), a 1 cm/h line was used to indicate an alert line on the partogram. All the active phase partograms were divided into five time zones: A (area to the left of the alert line), B (0–1 h after the alert line), C (1–2 h after the alert line), D (2–3 h after the alert line), and E and F (> 3 h after the alert line). For the action line, different lag times after the alert line were defined according to the time zones. Sensitivity, specificity, cesarean section rates and rupture rates were calculated for the different lag times after the alert line, and a receiver‐operating characteristic curve was constructed. Results: Fifty‐five (23.3%) trials of labor ended in a repeat emergency cesarean. There were seven (2.9%) cases of scar rupture. Of the 181 vaginal deliveries, 151 (83%) occurred within 2 h after the progress of labor had crossed the alert line (zones A‐C). Five out of seven cases of scar rupture occurred more than 2 h after the alert line had been crossed (zones D‐F). The 2‐ and 3‐h lag time after the alert line had a sensitivity of 71% and 43%, respectively, and a specificity of 78% and 96%, respectively, in predicting uterine scar rupture. If cesarean sections were performed at 2 or 3 h after crossing the alert line, the projected cesarean rates would have been 36% and 27%, respectively. The scar rupture rates would in turn be 0.8% and 1.6%, respectively. Conclusion: In women undergoing a trial of labor following cesarean section, the partographic zone 2–3 h after the alert line represents a time of high risk of scar rupture. An action line in this time zone would probably help reduce the rupture rate without an unacceptable increase in the rate of cesarean section.
Long‐term prognosis of acute pulmonary oedema — an ominous outcome
Authors: Roguin Ariel , Behar Doron M. , Ami Haim Ben , Reisner Shimon A. , Edelstein Shimon , Linn Shai , Edoute Yeouda 
Published: July 2002

AbstractBackground:Acute pulmonary oedema (APOE) is a major health problem, leading to poor hospital and long‐term outcomes. There is a relative paucity of studies describing prognosis of consecutive unsolicited patients diagnosed with APOE and hospitalized in internal medicine departments.Aims:To describe the clinical profile and outcome (in hospital and 1‐year prognosis) of successive unselected patients with APOE, in a prospective observational study.Methods and results:The study population included 150 consecutive unsolicited patients (90 men, 60 women; median age 75 years) with APOE all hospitalized in an internal medicine department, in a 900‐bed care centre. Ischaemic heart disease (IHD), hypertension and diabetes were present in 85%, 70% and 52% of patients, respectively. The most common precipitating factors for APOE included high blood pressure (29%), rapid atrial fibrillation (29%), unstable angina pectoris (25%), infection (18%) and acute myocardial infarction (MI; 15%). Eighteen patients (12%) died in hospital, with 82% of these deaths attributed to cardiac pump failure. Predictors for an increased in‐hospital mortality included: diabetes (P < 0.05), orthopnoea (P < 0.05), echocardiographic finding of depressed global left ventricular systolic function (P < 0.001), acute MI during hospital stay (P < 0.001), hypotension/shock (P < 0.05), and the need for mechanical ventilation (P < 0.001). After a median hospital stay of 10 days, 132 patients were discharged home. The 1‐year mortality was 40%. Only the presence of pleural effusion was found as a predictor for 1‐year mortality.Conclusion:Most patients with APOE in this study are elderly, and have IHD, hypertension, diabetes and a previous history of APOE. The overall mortality is high (in‐hospital, 12%: 1‐year, 40%). Left ventricular dysfunction was associated with high in‐hospital mortality, but not with long‐term prognosis.
Photoacclimation of Chlorella vulgaris to Red Light from Light‐Emitting Diodes Leads to Autospore Release Following Each Cellular Division
Authors: Lee Choul‐Gyun , Palsson Bernhard Ø. 
Published: July 2002

AbstractThe detailed light requirement for photosynthesis and photoautotrophic cell growth can be assessed using solid state technology. Advanced light‐emitting diodes (LEDs), constructed with double‐power double‐heterostructure (DDH) gallium aluminum arsenide (GaAlAs) chips, were examined for their ability to support mass culture of the eucaryotic alga Chlorella vulgaris. LEDs with peak emittance of 680 nm (with half‐power band width of 20 nm) were used as a sole light source for the cultivation of C. vulgaris. Fluorescent light (FL) served as a control. The final cell mass and specific cellular growth rate under LEDs were comparable to those obtained under full‐spectrum light (FL). The narrow‐spectrum monochromatic red light was found to reduce the average cell volume from 60 μm3 to 30 μm3, and to make the size distribution and the per cell DNA distribution narrower, but did not affect the total biomass production. By switching light sources, the two distinct cell population states (obtained under red LEDs and FL, respectively) were found to be interchangeable. Two parametric flow cytometric analyses showed that the cells grown under red LED light had a more uniform DNA content at all cell sizes, as compared to cells grown under FL. These results show that the critical cell size for releasing autospores under red LED is smaller than that under FL. The number of autospores in one mother cell when grown under LED light appeared to be two, so that the mother cells break up after only one round of DNA replication. Although the solid state LED light source altered the cell cycle behavior of C. vulgaris, it can be used as an effective light source for autotrophic growth. Use of LEDs therefore promises to advance the current state of algal photobioreactors due to their efficiency, smallness, reliability, long lifetime, and desirable light characteristics.
Comparison of Two Convection‐Aided Protein Adsorption Methods Using Porous Membranes and Perfusion Beads
Authors: Kubota Noboru , Konno Yoshitaka , Miura Suguru , Saito Kyoichi , Sugita Kazuyuki , Watanabe Kohei , Sugo Takanobu 
Published: July 2002

AbstractWe describe results which serve as a guide in the selection of protein recovery techniques using two new chromatographic methods based on anion‐exchange interaction, i.e., membrane and perfusion chromatographies, which involve enhancement of protein transport by convective flow. Bovine serum albumin solution was permeated through a functionalized porous hollow‐fiber membrane and a bed charged with functionalized porous (perfusion) beads of identical volume. The pores of the membrane are surrounded by grafted polymer chains immobilizing anion‐exchange groups, whereas the throughpores of the beads are surrounded by the diffusive pores at the periphery of which anion‐exchange groups are immobilized. An 8‐fold increase in throughput of the protein using the porous membrane, 37 mg of BSA/mL/min, was achieved at a low operating pressure of 0.1 MPa, compared to that using the perfusion bed.
Enteric Bacterial Catalysts for Fuel Ethanol Production
Authors: Ingram L. O. , Aldrich H. C. , Borges A. C. C. , Causey T. B. , Martinez Alfredo , Morales Fernando , Saleh Alif , Underwood S. A. , Yomano L. P. , York S. W. , Zaldivar Jesus , Zhou Shengde 
Published: July 2002

AbstractThe technology is available to produce fuel ethanol from renewable lignocellulosic biomass. The current challenge is to assemble the various process options into a commercial venture and begin the task of incremental improvement. Current process designs for lignocellulose are far more complex than grain to ethanol processes. This complexity results in part from the complexity of the substrate and the biological limitations of the catalyst. Our work at the University of Florida has focused primarily on the genetic engineering of Enteric bacteria using genes encoding Zymomonas mobilis pyruvate decarboxylase and alcohol dehydrogenase. These two genes have been assembled into a portable ethanol production cassette, the PET operon, and integrated into the chromosome of Escherichia coli B for use with hemicellulose‐derived syrups. The resulting strain, KO11, produces ethanol efficiently from all hexose and pentose sugars present in the polymers of hemicellulose. By using the same approach, we integrated the PET operon into the chromosome of Klebsiella oxytoca to produce strain P2 for use in the simultaneous saccharification and fermentation (SSF) process for cellulose. Strain P2 has the native ability to ferment cellobiose and cellotriose, eliminating the need for one class of cellulase enzymes. Recently, the ability to produce and secrete high levels of endoglucanase has also been added to strain P2, further reducing the requirement for fungal cellulase. The general approach for the genetic engineering of new biocatalysts using the PET operon has been most successful with Enteric bacteria but was also extended to Gram positive bacteria, which have other useful traits for lignocellulose conversion. Many opportunities remain for further improvements in these biocatalysts as we proceed toward the development of single organisms that can be used for the efficient fermentation of both hemicellulosic and cellulosic substrates.
Aggression and competition for shelter between a native and an introduced crayfish in Europe
Authors: Vorburger Christoph , Ribi Georg 
Published: July 2002

1. The introduced North American crayfish, Pacifastacus leniusculus Dana, is expanding its range in Europe and locally often replaces two native crayfish species, Astacus astacus L. and Austropotamobius pallipes Lereboullet. Pacifastacus leniusculus is also expected to invade the habitat of a third native crayfish, the endangered Austropotamobius torrentium Schrank. Interspecific aggressive interactions and competition for shelter were experimentally studied in the laboratory to assess the potential impact of P. leniusculus on A. torrentium.2. Neither species was inherently dominant in aggressive interactions, but dominance was strongly size‐dependent, favouring the larger and faster growing species, P. leniusculus.3. Access to limited shelter was generally determined by aggressive dominance, although species‐specific preference also influenced the outcome of competition for shelter. Austropotamobius torrentium had a higher preference for experimental shelters and often defended these even against larger P. leniusculus.4. In accordance with theoretical models of animal conflicts, agonistic interactions between equally sized contestants were more severe than between animals of different size.5. The P. leniusculus used in the present experiment were infected with the crayfish plague, Aphanomyces astaci, to which the animals are resistant. The crayfish transmitted the disease to non‐resistant A. torrentium which died ≈ 2 weeks after contact with P. leniusculus.