bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multifunctional cotton fabric with durable antibacterial, superhydrophobicity, and UV resistance based on Ag@TiO 2 Janus nanoparticles
Authors: Gao Dangge , Wang Fangxing , Lyu Bin , Ma Jianzhong , Zhao Zhouyang 
Published: July 2023

Abstract
The market demand for multifunctional cotton fabric is increasing. However, the key of developing cotton fabric with multiple functions is how to solve the problem of functional combination. In this study, silver@titanium dioxide Janus nanoparticles (Ag@TiO2 Janus nanoparticles) were synthesized by Pickering emulsion polymerization and finished on the epoxy modified cotton fabric (Ag@TiO2 Janus/E-cotton fabric). The Ag@TiO2 Janus nanoparticles had asymmetric Janus structure, that one side being silane with hydrophilic amino group was covalently bonded with the epoxy group on the cotton fabric fibers and the other side being silane with hydrophobic long-chain alkane was faced the environment, which was to endow the cotton fabric durably superhydrophobic, UV resistance, and antibacterial. Characterization by SEM, XRD, EDS, EDS, FT-IR and TG verified the finishing of the cotton fabric with Ag@TiO2 Janus nanoparticles. Ag@TiO2 Janus/E-cotton fabric had a water contact angle of 160, after 50 wear cycles, the contact angle at the damaged part could still reach 152. Compared with raw cotton fabric, the antibacterial rate of Ag@TiO2 Janus/E- cotton fabric to Escherichia coli (E. coli) and Streptococcus Urealyticus (S. aureus) is more than 95%. After 8 ultrasonic washing cycles, the antibacterial rate still maintained more than 80%. The UV protection performance of the finished cotton fabric was improved by 82.3%.
Generation and characterization of a zebrafish knockout model of abcb4, a homolog of the human multidrug efflux transporter P-glycoprotein
Authors: Park Jinhee , Kim Hyosung , alabdalla Leen , Mishra Smriti , Mchaourab Hassane 
Published: July 2023

Abstract
The ATP-binding cassette subfamily B member 1 (ABCB1), encoding a multidrug transporter referred to as P-glycoprotein (Pgp), plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy. Therefore, developing high throughput animal models to screen for Pgp function and bioavailability of substrates and inhibitors is paramount. Here, we generated and validated a zebrafish knockout line of abcb4, a human Pgp transporter homolog. CRISPR/Cas9 genome editing technology was deployed to generate a frameshift mutation in exon 4 of zebrafish abcb4. The zebrafish abcb4 homozygous mutant exhibited elevated accumulation of fluorescent rhodamine 123, a substrate of human Pgp, in the intestine and brain area of embryos. Moreover, abcb4 knockout embryos were sensitized toward toxic compounds such as doxorubicin and vinblastine compared to the WT zebrafish. Immuno-staining for zebrafish Abcb4 colocalized in the endothelial brain cells of adult zebrafish. Transcriptome profiling using Gene Set Enrichment Analysis (GSEA) uncovered that the 'cell cycle process,' 'mitotic cell cycles,' and 'microtubule-based process' were significantly downregulated in the abcb4 knockout brain with age. This study establishes and validates the abcb4 knockout zebrafish as an animal model to study Pgp function in vivo. Unexpectedly it reveals a potentially novel role for zebrafish abcb4 in age-related changes in the brain. The zebrafish lines generated here will provide a platform to aid in the discovery of modulators of Pgp function as well as the characterization of human mutants thereof.
Tumor antigens and immune landscapes identification guided the development of colorectal cancer mRNA vaccines
Authors: 唐 剑 , Yang De'zheng , Liu Zhixian , Wei Jifu 
Published: July 2023

Abstract
Colorectal cancer (CRC) is the third most common tumor and the second leading cause of cancer-related mortality. As an alternative to traditional cancer immunotherapy approaches, mRNA vaccines have gained significant attentio due to their numerous advantages. In this study, our objective was to screen for potential tumor antigens in CRC and identify mRNA vaccines capable of targeting specific immune subtypes based on their recognition of these immune subtypes. Through our research, We successfully identified eight overexpressed and mutated tumor antigens associated with poor prognosis in CRC, including ADAMTS4, LZTS1, OLR1, SLC11A1, SPOCD1, SPP1, STC1 and TIMP1. Then we assessed the association between these genes and the antigen-presenting immune cells. Furthermore, we identified three distinct immune subtypes of CRC, namely CRC immune subtype (CIS) 1–3. Among these subtypes, CIS3 exhibited a worse prognosis, a higher number of tumor mutations, and significantly lower immune activity compared to CIS1 and CIS2. Additionally, these above immune types were prominently linked to different immunocompetencies, immune genic cell death modulators and the prognostic factors in CRC. In summary, our findings conclusively identify three distinct immune subtypes of CRC and eight potential targets for CRC mRNA vaccines. These findings provide a new perspective on antigen selection and population stratification for future development and application of CRC mRNA vaccines.
Effects of maternal dietary enteromorpha prolifera polysaccharide iron supplement on mineral elements and iron level of neonatal piglets
Authors: Liu Hong-wei , Liu Gang-yi , Wang Hai-hua , Gao Lu-min , Xie Chun-yan , Wu Xin 
Published: July 2023

Abstract
Iron plays a key role in maternal health during pregnancy and fetal growth. Enteromorpha polysaccharide –iron (EP-Fe) as an organic iron chelate may improve the iron transmission of mother and offspring, ameliorate the poor pregnancy outcomes of sows, and alleviate the growth restriction of piglets caused by iron deficiency. This study aimed to evaluate the effects of maternal dietary supplementation with EP-Fe on reproductive performance and placental iron transmission of sows, as well as growth performance of piglets. Sixty pregnant sows at the 95th day of gestation were randomly divided into control group (CON) and EP-Fe group (EP-Fe, 139 mg kg− 1). Blood samples of sows and neonatal piglets, colostrum, and tissue samples were collected on the day of delivery. The animal experiment ended at the 21th day of post-delivery. Results showed that maternal dietary EP-Fe increased colostrum iron (P < 0.05) of sows, as well as final litter weight (P < 0.05) and average daily weight of piglets (P < 0.05) during d 1–21 of lactation, as well as iron and manganese content in umbilical cord blood (P < 0.05) and hepatic iron of neonatal piglets (P < 0.01), and decreased fecal iron (P < 0.001), serum calcium (P < 0.05), phosphorus (P < 0.05) and zinc (P < 0.01) in the parturient sow. RT-qPCR results showed that FPN1 and SLC39A14 in placenta, as well as TfR1 and SLC39A14 in duodenum of neonatal piglets were activated by maternal EP-Fe supplement. These findings suggest that maternal dietary EP-Fe could increase iron storage of neonatal piglets via improving placental iron transport and iron secretion in colostrum, thus enhance the growth performance of sucking piglets.
A serum panel of three microRNAs may serve as a possible biomarker for Kidney renal clear cell carcinoma
Authors: Wen Zhenyu , Li Yingqi , Zhao Zhengping , Li Rongkang , Li Xinji , Lu Chong , Sun Chen , Chen Wenkang , Ge Zhenjian , Ni Liangchao , Lai Yongqing 
Published: July 2023

Abstract
Background: Approximately 75 percent of cases of renal cell carcinoma, also known as kidney renal clear cell carcinoma (KIRC), are diagnosed during procedures to identify renal cell carcinoma (RCC). Although non-invasive radiological techniques are widely applied in the diagnoses of KIRC, ˃ 50% of KIRCs are currently detected incidentally. The presence of microRNA (miRNA) in serum has the potential to serve as a non-invasive diagnostic biomarker for KICC.
Method: In the three-part trial, there were a total of 224 participants, 112 of them were KIRC patients (KIRCs), while the other 112 were healthy controls (NCs). RT-qPCR was utilized so that we could evaluate the level of miRNA expression in both KIRCs and NCs. ROC curves and the area under the ROC curve (AUC) were utilized in order to make a prediction regarding the usefulness of serum miRNAs for KIRC diagnosis. In addition, we performed survival analysis and bioinformatics with our research.
Results: We discovered that miR-1-3p, miR-129-5p, miR-146b-5p, miR-187-3p and miR-200a-3p showed statistically significant differences when compared to NCs. It was determined that a panel consisting of three microRNAs (miR-1-3p, miR-129-5p and miR-146b-5p) had an AUC of 0.895, with a 95% confidence interval ranging from 0.848 to 0.942. CREB5 may be a potential target gene of the panel in the GEPIA database. According to the survival analysis, the overexpression of miR-146b-5p was indicative of a poorer prognosis for KIRC patients.
Conclusions:There is a significant possibility that the three-miRNA panel in the serum could serve as non-invasive indicators for KIRC.
Potential of Protein Risk Signatures in Lung Adenocarcinoma: A Comprehensive Bioinformatics Study
Authors: Yu Xiaofei , Zheng Lei , Xia Zehai , Xu Yanling , Shen Xihui , Huang Yihui , Dai Yifan 
Published: July 2023

Abstract
Purpose
Global leading cause of cancer deaths, lung adenocarcinoma (LUAD), is the focus of our study, which explores a protein-based risk signature for LUAD prognosis.
Methods
Utilizing the Cancer Genome Atlas, we used LASSO-COX to create a prognostic protein model. The model's effectiveness and clinical relevance were analyzed, and a predictive nomogram was built. Concurrently, potential pathways were identified through Gene Set Enrichment Analysis (GSEA) and the tumor immune microenvironment was examined alongside immunotherapy sensitivity. We corroborated model protein expression with immunohistochemistry images from the HPA database and immunofluorescence staining of clinical samples.
Results
Our six-protein model stratifies LUAD patients effectively into risk groups and shows strong pre-dictive power. The nomogram forecasts overall survival rates at one, three, and five-year intervals. GSEA highlighted enrichment of high-risk genes in metabolic pathways and low-risk genes in immune-related pathways—the latter indicating greater immunotherapy sensitivity. Validation experiments revealed high CD38 expression in lung cancer, presenting a novel paradox.
Conclusion
This study offers a valuable prognostic protein model for LUAD and uncovers a CD38 expression paradox, enhancing our understanding of protein involvement in lung cancer progression.
Arabic and Latin license plate detection and recognition based on YOLOv7 and image processing methods
Authors: Moussaoui Hanae , AKKAD Nabil EL , BENSLIMANE Mohamed 
Published: July 2023

Abstract
License plate detection has always been challenging, especially with the increasing number of smart radars on roads and highways. By 2021, over 1 billion smart traffic radars have been provided worldwide for the gendarmerie as well as for road control officers from the National Security. This paper introduces a novel Arabic and Latin license plate detection and recognition method. In the first stage of the proposed method and after gathering images to build a new dataset, we used YOLOv7 to detect and localize the license plate in the image. The dataset has been labeled manually before feeding it to the detection system. Second, we applied some of the machine learning algorithms to enhance the detected license plate. For this, we employed thresholding as well as the kernel algorithms in order to remove the additional vertical lines in the plate. Afterward, we used the Arabic OCR as well as Easy OCR techniques to recognize the Arabic and the Latin letters on the license plate. The proposed detection algorithm achieved a precision of 97% and a recall of 98%, consequently, we achieved an F1 score of 98% in license plate detection. For image segmentation, while using Arabic OCR and Easy OCR to segment and extract characters from the detected license plate, we achieved an accuracy of 99 %. The proposed method gave satisfactory results for both detection and segmentation.
Perioperative and Recurrence-Free Survival Outcomes after Laparoscopic Hepatectomy for Colorectal Cancer Liver Metastases Using Indocyanine Green Fluorescence Imaging: A Single-Center Experience
Authors: Wang Gaoming , Liu Chenghao , Qi Weijun , Li Long , Yuan Chunhui , Xiu Dianrong 
Published: July 2023

Abstract
Background
Indocyanine green (ICG) fluorescence imaging has been extensively used in the context of laparoscopic hepatectomy for colorectal cancer liver metastases (CRLM). Nevertheless, there remains a lack of strong evidence on the influence of perioperative and survival outcomes in patients undergoing such procedure.
Methods
The retrospective cohort study was conducted on consecutive patients with CRLM who underwent laparoscopic hepatectomy. The patients were divided into two groups based on utilization of ICG fluorescence imaging during surgical procedure. Data on sociodemographic characteristics, laboratory parameters, pathology findings, surgical outcomes, and survival results were systematically collected for analysis.
Results
A total of 117 patients were involved in the analysis. Compared to the traditional groups, the ICG-guided group had a significantly higher rate of negative margin (97% v.s 86%, p = 0.009) and lower rate of postoperative bile leakage (13% v.s 31%, p = 0.018). ICG fluorescence imaging was determined to be an independent risk factor associated with postoperative bile leakage (OR = 0.035, 95%CI 0.114–0.982, p = 0.046). As of the last recorded follow-up date, there were 82 (76.6%) recurrences in the entire cohort. No significant difference was found between the two groups in terms of early recurrence rate (26.3% v.s 40%, p = 0.132). However, the intrahepatic recurrence rate was significantly lower in the ICG-guided group (45.6% v.s 68.0%, p = 0.02). The median recurrence free survival of patients in the ICG-guided group was significantly longer compared to the traditional group (11.3 v.s 7.2 months, p = 0.041).
Conclusion
ICG fluorescence imaging can be utilized to delineate tumor boundaries and determine surgical margin during surgical procedures for CRLM. Additionally, it has shown promising potential in improving the perioperative and recurrence-free survival outcomes in these patients.
High-expressed ACAT2 predicted the poor prognosis of platinum-resistant epithelial ovarian cancer
Authors: Wang Jinfeng , Yang Zhe , Zhao Lanbo , Bin Yadi , Zhang Siyi , Ji Jing , Liu Jinren , Wang Yiran , Li Min , Liu Yu , Hou Huilian , Li Qiling 
Published: July 2023

Abstract
Background Acetyl-CoA acetyltransferase 2 (ACAT2) is a lipid metabolism enzyme and rarely was researched in epithelial ovarian cancer (EOC).
Methods ACAT2 expressions were confirmed by quantitative real-time polymerase chain reaction and western blotting in SKOV3, SKOV3/DDP, A2780 and A2780/DDP cell lines. Tissue samples were stained by immunohistochemistry and scored for ACAT2 expression. The relationships between ACAT2 expression and clinicopathological characteristics were analyzed by χ2 test. The prognosis of ACAT2 was analyzed by log-rank tests and Cox regression models.
Results ACAT2 was remarkably upregulated in the above drug-resistant cell lines by mRNA (P < 0.001) and protein expression (P < 0.05) than those in sensitive ones. Patients were classified as ACAT2-high (n = 51) and ACAT2-low (n = 26) according to immunohistochemical score. ACAT2 expression had a significantly inverse correlation with FIGO stage (P = 0.030) and chemo-response (P = 0.041). A marginal statistical significance existed in ACAT2 expression and ascites volume (P = 0.092). Univariate analysis suggested that high-expressed ACAT2 was associated with decreased platinum-free interval (PFI) (8.57 vs 14.13 months, P = 0.044), progression-free survival (PFS) (14.12 vs 19.79 months, P = 0.039) and overall survival (OS) (36.89 vs 52.40 months, P = 0.044). Multivariate analysis demonstrated that ACAT2 expression (hazard ratio = 2.18, 95% confidence interval: 1.15 - 4.11, P = 0.017) affected OS independently, rather than PFI and PFS.
Significance High-expressed ACAT2 was associated with advanced FIGO stage, chemo-resistance, and decreased PFI, PFS and OS. It was an independent prognostic factor of OS in EOC.
Identification of biomarkers associated with immune-propionate metabolism in nonalcoholic fatty liver disease
Authors: Qu Shuye , Huang Hui , Diao Yan , Liu Bowei , Tang Baozhu , Huo Shijiao , Lei Yu , Xuan Xiuchen , Mou Wenling , Liu Ping , Zhang Jiye , Liu Ying , Li Yanze 
Published: July 2023

Abstract
Background: The mechanisms of the effect of propionate metabolism and immunity on nonalcoholic fatty liver disease (NAFLD) have not been adequately studied.
Methods: Firstly, differentially expressed-propionate metabolism-related genes (DE-PMRGs) were selected by overlapping PMRGs and differentially expressed genes (DEGs) between the simple steatosis (SS) and health control (HC) groups. Then, common genes were selected by overlapping DE-PMRGs and key module genes obtained from weighted gene co-expression network analysis (WGCNA). Subsequently, the biomarkers were screened out by machine learning algorithms. The expression of the biomarkers was validated by quantitative Real-time PCR.
Results: In total, 5 biomarkers (JUN, LDLR, CXCR4, NNMT, and ANXA1) were acquired. The nomogram constructed based on 5 biomarkers had good predictive power for the risk of SS. Next, 5 biomarkers, 11 miRNAs, and 149 lncRNAs were encompassed in the ceRNA regulatory network. The expression of biomarkers was significantly higher in the HC group than in the SS group, which was consistent with the results in the GSE89632 and GSE126848 datasets.
Conclusions: In this study, 5 immune and propionate metabolism-related biomarkers (JUN, LDLR, CXCR4, NNMT, and ANXA1) were screened out to provide a basis for exploring the prediction of diagnosis of NAFLD.
Influence of malignant left large bowel obstruction in metastatic left-sided colon cancer patients during chemotherapy on survival: A Propensity Score Analysis
Authors: Xiao Jianlin , shen yu , Yang Xuyang , Zeng Hanjiang , Wei Mingtian , Meng Wenjian , Wang Ziqiang 
Published: July 2023

AbstractBackgroundChemotherapy was recommended to improve the long-term outcomes of metastatic colorectal cancer (mCRC). The process of chemotherapy may be interrupted by malignant large bowel obstruction (MLBO). The aim of this study was to find out the Oncologic outcomes for MLLBO in metastatic left-sided colon cancer (mLCC) patients during chemotherapy.MethodsConsecutive mLCC patients who underwent chemotherapy from June 2009 to July 2021 in West China Hospital were divided into two groups according to whether MLLBO occurred during chemotherapy. Patients were propensity matched 1:2 into MLLBO and no-MLLBO groups and among the matched cohort, the univariable and multivariable Cox proportional hazards regression models were performed to identify predictors of survival. Potential variables were evaluated first with univariate and then multivariate analyses to identify the risk factors of tumor characteristics for MLLBO patients.ResultsOf 1638 mLCC patients diagnosed, 156 were identified in the matched cohort. The median overall survival was 34.0 months, 51.0 months in the non-MLLBO, and MLLBO groups, respectively (p= 0.020) and the corresponding median progression-free survival was 12.0 months, 23.0 months, respectively (p=0.018). Multivariable Cox regression analysis demonstrated that MLLBO was independently associated with reduced overall survival (hazard ratio [HR]= 1.473, 95% CI 1.186-1.812, P＜0.001) as well as progression-free survival (hazard ratio [HR]= 1.619, 95% CI 1.391-1.899, P＜0.001). In addition, Tumor length ≥ 10 cm (P=0.043) and TTI ≥ 1.5 cm (P＜0.001) were identified as independent risk factors of tumor characteristics for MLLBO patients.ConclusionNo-MLLBO patients had better prognosis than MLLBO patients during chemotherapy. Prevention measures are recommended for mLCC patients with higher risk factors of MLLBO during chemotherapy.
Comparison of surgical site infection (SSI) between Negative Pressure Wound Therapy (NPWT) assisted delayed primary closure and conventional delayed primary closure in grossly contaminated emergency abdominal surgeries: a randomised controlled trial
Authors: Singh Pradeep Kumar , Sethi Mahesh Kumar , Mishra Tushar Shubhadarshan 
Published: July 2023

Abstract

Purpose
NPWT has been tried in many surgical fields including colorectal, thoracic and vascular, and non-healing wounds for prevention of SSI. But its efficacy on prevention of SSI grade IV closed abdominal wound yet to be explored.
Methods
All patients with grade-IV abdominal wound were included in the study. They were randomised into conventional arm and VAC arm after confirming the diagnosis intra-operatively. The sheath was closed and skin laid open in post-operative period. In VAC arm, the NPWT dressing applied on post-operative day (POD)-1 and it was removed on POD-5. In conventional arm, only regular dressing was done postoperatively. The skin was closed with delayed primary intention on POD-5 in both the arms. The sutures were removed after 7 to 10 days of skin closure.
Results
The rate of SSI (10% in VAC arm vs 37.5% in conventional arm, p-value = 0.004) was significantly lower in VAC arm, as well as the rate of seroma formation (2.4% in VAC arm vs 20% in conventional arm, p = 0.014) and wound dehiscence (7.3% vs 30%, p = 0.011). The conventional arm had significantly delay in skin closure beyond POD5 due to increased rate of SSI and also lead to prolonged hospital stay (5days in VAC arm vs 6.5days in conventional arm, p-value = 0.005).
Conclusion
The VAC dressing can be used routinely in grade IV closed abdominal wounds to prevent SSI and other post-operative wound related complications.
New analytical solutions to the stochastic (2 + 1)-dimensional combined KdV-mKdV equation
Authors: Rosas Miguel , Saavedra Carlos , Morales Benjamín 
Published: July 2023

Abstract
In this article, we investigate the (2 + 1)-dimensional stochastic KdV-mKdv equation perturbed by white noise in the Itô sense. The improved Tanh-Coth method is used to obtain new stochastic solutions. It also extends some previous research. Since this new equation is essential for describing various phenomena in ocean engineering, hydrodynamics and optics, the obtained solutions are useful for interpreting certain interesting physical phenomena. To show how multiplicative white noise affects the exact solution, we plot the solutions in Julia and show some 3D plots.