bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Central Arctic Ocean fisheries moratorium: A rare example of the precautionary principle in fisheries management
Authors: Calderwood Cayla , Ulmer Frances Ann 
Published: January 2023

AbstractOn 25 June 2021, a historic fisheries Agreement entered into force: The Agreement to Prevent Unregulated High Seas Fisheries in the Central Arctic Ocean (CAO). Nine countries and the European Union agreed to refrain from any commercial fishing in the CAO and to jointly undertake a scientific effort to understand ecosystem dynamics, including fish populations. This was the first multilateral Agreement to take a legally binding, precautionary approach to protect an area from commercial fishingbeforefishing had begun. The Agreement is a textbook example of the precautionary principle as it works to take “preventive action in the face of uncertainty.” However, despite the precautionary principle’s popularity with natural resource academics, it is rare for countries to forego economic benefits and to adopt this approach in managing resources. So, what made this Agreement possible? And what can we learn from this Agreement that could provide guidance on other resource management challenges? This paper explores the unique conditions that made this Agreement possible and examines how success was achieved by the interrelationships of science, policy, legal structures, politics, stakeholder collaboration, and diplomacy. In summary, this paper concludes that a series of factors helped make this Agreement possible, including but not limited to: scientific breakthroughs coupled with science-based legal frameworks; proactive partnerships between industry, environmental non-profits, and government; willingness of international stakeholders to learn from prior mistakes; and a nation willing to be the first-mover in foregoing future economic profits within their own Exclusive Economic Zone to order to benefit ecosystems beyond their waters.
Informing decision makers about public preferences for different modalities of cancer treatment in the Rhône–Alps region in France
Authors: Margier Jennifer , Gafni Amiram , Moumjid Nora 
Published: January 2023

Abstract

Background
Alternative options to hospital care like home care or local health centers (LHCs) are being advocated. However, no study has measured citizens’ preferences (who will finance these services via taxation) for these options.

Objectives
We measured (i) citizens’ preferences for these services, that is, respondents stated where they would like to get the treatment; (ii) the strength of their preference.

Methods
A computerized survey composed of (i) a decision aid to inform respondents about the three options; (ii) three scenarios, from light-to-heavy care, that respondents should rank from the most to the least preferred option of care. (iii) a contingent valuation survey (CVS) to assess how much respondents were willing to pay for their preferred option (except for hospital care if chosen, because it is the default option and free). (iv) a socio-demographic questionnaire.

Results
Data were collected from a representative sample of citizens living in the Rhône–Alps Region (n = 800). The heavier the care was, the more respondents preferred hospital care. Willingness to pay for additional taxation per household/month varied from €13.9 for light care in LHC to €19.1 for heavy home care. The small number of protesting respondents and outliers, and the close correlation between preferences, income, and WTP supports the validity of the CVS.

Conclusion
In France, for cancer, not all citizens would prefer to be treated at home rather than in a hospital. Only less than a quarter would prefer LHC. These results show the mismatch between public health policies and the citizens’ preferences.
Governing Rural Poverty on Urban Streets: Guangzhou's Management of Beggars in the Reform Era
Authors: Flock Ryanne 
Published: January 2023

AbstractThis study investigates how discourses on panhandling intertwine with the governance of beggars on China's urban streets. It focuses on local policy implementation in Guangzhou city, led by the bureau of civil affairs along with its centres for “custody and repatriation” and “assistance stations.” The study aims to understand how the state regulates panhandling and engages with beggars in public spaces. Exploring the internal logic of the state's approach and how it has changed during the 40 years of reform, it also considers the junctures at which contradictions and conflicts arise. Based on fieldwork data (2011 to 2014) and the analysis of government documents, yearbooks, academic and mass media discourses, I argue that the state's treatment of panhandlers poses a conundrum as welfare measures conflict with control. While several layers of state regulation and actors contradict each other and create grey areas of state-induced informality, people who beg for alms are continuously criminalized and excluded from public space.
Improvement of helical tomotherapy treatment plan efficiency with block techniques for left-sided post-mastectomy radiation therapy
Authors: Chirawattana Arisara , Chitapanarux Imjai , Nobnop Wannapha 
Published: January 2023

Abstract

Purpose:
To limit the entrance dose to normal tissue and achieve the appropriate treatment time (TT) by using three different virtual structures with directional blocks for left-sided post-mastectomy radiation therapy (PMRT) with regional nodal irradiation (RNI).

Methods and materials:
Ten breast cancer patients who received PMRT by helical tomotherapy were enrolled. Three virtual structures were created for each patient: Organ-based, L-shaped (LB) and C-shaped (CB). The dose to the target and organ at risk (OARs), TT, the volume which received dose 5 Gy (V5Gy), integral dose (ID) and block structure contouring workload (BSCW) of the three virtual block techniques were evaluated. The performance scores were used to explore the suitable technique.

Results:
The CB plans showed a significantly better V5Gy, ID and contralateral breast-sparing. However, the CB plans revealed the longest TT and BSCW (p < 0·001). Contrary to the LB, the LB plans showed a significantly reduced TT and BSCW and provided the balance of plan efficiency with the highest score.

Conclusion:
The LB technique is considered to be the suitable technique for left-sided PMRT with RNI and provided the advantage of TT, V5Gy, ID and BSCW while maintaining acceptable criteria for the target and OARs.
Längsschnittliche psychometrische Analysen des Fragebogens zur Beziehungszufriedenheit – Kurzform (FBZ-K)
Authors: Hahlweg Kurt , Schulz Wolfgang 
Published: January 2023

Zusammenfassung: Kurze diagnostische Instrumente zur Erfassung von Beziehungsqualität sind für den klinischen Alltag notwendig, wie z. B. die sieben Items umfassende Abbreviated Dyadic Adjustment Scale (ADAS; dt.: Fragebogen zur Beziehungszufriedenheit – Kurzform, FBZ-K). Ziel ist es, die psychometrische Qualität der FBZ-K zu untersuchen und mit den Daten der ADAS zu vergleichen. Für den Vergleich wurden in einer ersten Studie 422 Mütter rekrutiert. In einer zweiten Studie wurden Daten von 424 Eltern (223 Mütter, 201 Väter) verwendet, die im Rahmen einer Langzeitstudie über 10 Jahre zu sechs Messzeitpunkten untersucht wurden. Die psychometrischen Kennwerte der FBZ-K entsprachen denen der ADAS. Die interne Konsistenz mit α = .84 und die 1-Jahres-Retest-Reliabilität mit r = .75 sind gut, die Konstrukt- und prognostische Validität zur Vorhersage von Scheidung kann als in hohem Maße als gegeben gelten. Die FBZ-K kann daher als Screening-Instrument in epidemiologischen Studien und in der klinischen Praxis zur Eingangsmessung und Prozessforschung empfohlen werden.
Mechanistic Analysis and Numerical Simulation of the 2021 Post‐Fire Debris Flow in Xiangjiao Catchment, China
Authors: Ouyang Chaojun , Xiang Wen , An Huicong , Wang Fulei , Yang Weibin , Fan Jiying 
Published: January 2023

AbstractWildfires are becoming a global natural disaster under climate change. A worthwhile concern is that the rainfall thresholds that trigger debris flows in catchments affected by recent wildfires are much lower than those that trigger debris flows in catchments with similar environments but that have not experienced wildfires. In this study, we investigated a major post‐fire debris flow event in Xiangjiao catchment triggered by heavy rainfall in 2021. Through the combination of field observation, laboratory experiments and several numerical models (rainfall interception, infiltration, erosion and runoff models), the physical mechanistic analysis and numerical simulation of the post‐fire debris flow in Xiangjiao catchment was carried out. Results show that the simulated deposited area, erosion depth, and the occurrence time of debris‐flow initiation correlate well with the actual situation. It is demonstrated that the numerical simulation method used in this study can be used to effectively describe the characteristics of the initial and propagation stages of the post‐fire debris flow generated by runoff and provide important insights and guidance for the prediction and prevention of debris‐flow disasters in catchments affected by wildfire.
Dual‐Band Reflectarray Antennas Using Integrated Resonant and Non‐Resonant Natures of Metallic Waveguide Elements at Millimeter Wave Frequencies
Authors: Chou H.‐T. , Wang N.‐N. , Fang M. , Wang L.‐Q. , Akkaraekthalin P. , Torrungrueng D. 
Published: January 2023

AbstractPure metallic reflectarray antennas have good power efficiency and low fabrication cost, limited to single‐band operation in the past. A dual‐band reflectarray antenna is developed for two directional beams by two feeds. The reflecting elements are metal waveguides and have novel properties of both resonant and non‐resonant modes at the two frequency bands. The low and high‐frequency bands can be easily separated by the cutoff frequency of waveguide modes. The phase‐changing mechanisms via ray optics and fundamental waveguide modes, respectively, provide simple formulations for the elemental structure design of directional beams. Radiation characteristics of the linear and circular polarizations are cross‐examined with a good performance by full‐wave simulations using HFSS, FEKO, and CST at 28 and 60 GHz bands for 5G front‐haul network applications. In particular, we have used HFSS to simulate the reflecting elements' basic characteristics of phase variation, FEKO to simulate the linear polarization antenna case, and CST to simulate the circular polarization antenna case, respectively.
Lateral septum-lateral hypothalamus circuit dysfunction in comorbid pain and anxiety
Authors: Wang Di , Pan Xiangyu , Zhou Yu , Wu Zifeng , Ren Kunpeng , Liu Hanyu , Huang Chaoli , Yu Yumei , He Teng , Zhang Xiao , Yang Ling , Zhang Hongxing , Han Ming-Hu , Liu Cunming , Cao Jun-Li , Yang Chun 
Published: January 2023

AbstractPain and anxiety comorbidities are a common health problem, but the neural mechanisms underlying comorbidity remain unclear. We propose that comorbidity implies that similar brain regions and neural circuits, with the lateral septum (LS) as a major candidate, process pain and anxiety. From results of behavioral and neurophysiological experiments combined with selective LS manipulation in mice, we find that LS GABAergic neurons were critical for both pain and anxiety. Selective activation of LS GABAergic neurons induced hyperalgesia and anxiety-like behaviors. In contrast, selective inhibition of LS GABAergic neurons reduced nocifensive withdrawal responses and anxiety-like behaviors. This was found in two mouse models, one for chronic inflammatory pain (induced by complete Freund’s adjuvant) and one for anxiety (induced by chronic restraint stress). Additionally, using TetTag chemogenetics to functionally mark LS neurons, we found that activation of LS neurons by acute pain stimulation could induce anxiety-like behaviors and vice versa. Furthermore, we show that LS GABAergic projection to the lateral hypothalamus (LH) plays an important role in the regulation of pain and anxiety comorbidities. Our study revealed that LS GABAergic neurons, and especially the LSGABAergic-LH circuit, are a critical to the modulation of pain and anxiety comorbidities.
Quantifying the local adaptive landscape of a nascent bacterial community
Authors: Ascensao Joao A. , Wetmore Kelly M. , Good Benjamin H. , Arkin Adam P. , Hallatschek Oskar 
Published: January 2023

AbstractThe fitness effects of all possible mutations available to an organism largely shape the dynamics of evolutionary adaptation. Yet, whether and how this adaptive landscape changes over evolutionary times, especially upon ecological diversification and changes in community composition, remains poorly understood. We sought to fill this gap by analyzing a stable community of two closely related ecotypes (“L” and “S”) shortly after they emerged within theE. coliLong-Term Evolution Experiment (LTEE). We engineered genome-wide barcoded transposon libraries to measure the invasion fitness effects of all possible gene knockouts in the coexisting strains as well as their ancestor, for many different, ecologically relevant conditions. We find consistent statistical patterns of fitness effect variation across both genetic background and community composition, despite the idiosyncratic behavior of individual knockouts. Additionally, fitness effects are correlated with evolutionary outcomes for a number of conditions, possibly revealing shifting patterns of adaptation. Together, our results reveal how ecological and epistatic effects combine to shape the adaptive landscape in a nascent ecological community.
Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways
Authors: Gong Yulong , Yang Bingyuan , Zhang Dingdong , Zhang Yue , Tang Zihan , Yang Liu , Coate Katie C. , Yin Linlin , Covington Brittney A. , Patel Ravi S. , Siv Walter A. , Sellick Katelyn , Shou Matthew , Chang Wenhan , Danielle Dean E. , Powers Alvin C. , Chen Wenbiao 
Published: January 2023

AbstractGlucagon has emerged as a key regulator of extracellular amino acid (AA) homeostasis. Insufficient glucagon signaling results in hyperaminoacidemia, which drives adaptive proliferation of glucagon-producing α cells. Aside from mammalian target of rapamycin complex 1 (mTORC1), the role of other AA sensors in α cell proliferation has not been described. Here, using both genders of mouse islets and glucagon receptor (gcgr)-deficient zebrafish (Danio rerio), we show α cell proliferation requires activation of the extracellular signal-regulated protein kinase (ERK1/2) by the AA-sensitive calcium sensing receptor (CaSR). Inactivation of CaSR dampened α cell proliferation, which was rescued by re-expression of CaSR or activation of Gq, but not Gi, signaling in α cells. CaSR was also unexpectedly necessary for mTORC1 activation in α cells. Furthermore, coactivation of Gq and mTORC1 induced α cell proliferation independent of hyperaminoacidemia. These results reveal another AA-sensitive mediator and identify pathways necessary and sufficient for hyperaminoacidemia-induced α cell proliferation.
Analysis of transcriptional changes in the immune system associated with pubertal development in a longitudinal cohort of children with asthma
Authors: Resztak Justyna A. , Choe Jane , Nirmalan Shreya , Wei Julong , Bruinsma Julian , Houpt Russell , Alazizi Adnan , Mair-Meijers Henriette E. , Wen Xiaoquan , Slatcher Richard B. , Zilioli Samuele , Pique-Regi Roger , Luca Francesca 
Published: January 2023

AbstractPuberty is an important developmental period marked by hormonal, metabolic and immune changes. Puberty also marks a shift in sex differences in susceptibility to asthma. Yet, little is known about the gene expression changes in immune cells that occur during pubertal development. Here we assess pubertal development and leukocyte gene expression in a longitudinal cohort of 251 children with asthma. We identify substantial gene expression changes associated with age and pubertal development. Gene expression changes between pre- and post-menarcheal females suggest a shift from predominantly innate to adaptive immunity. We show that genetic effects on gene expression change dynamically during pubertal development. Gene expression changes during puberty are correlated with gene expression changes associated with asthma and may explain sex differences in prevalence. Our results show that molecular data used to study the genetics of early onset diseases should consider pubertal development as an important factor that modifies the transcriptome.
Large-area waterproof and durable perovskite luminescent textiles
Authors: Tian Tian , Yang Meifang , Fang Yuxuan , Zhang Shuo , Chen Yuxin , Wang Lianzhou , Wu Wu-Qiang 
Published: January 2023

AbstractLead halide perovskites show great potential to be used in wearable optoelectronics. However, obstacles for real applications lie in their instability under light, moisture and temperature stress, noxious lead ions leakage and difficulties in fabricating uniform luminescent textiles at large scale and high production rates. Overcoming these obstacles, we report simple, high-throughput electrospinning of large-area (> 375 cm2) flexible perovskite luminescent textiles woven by ultra-stable polymer@perovskite@cyclodextrin@silane composite fibers. These textiles exhibit bright and narrow-band photoluminescence (a photoluminescence quantum yield of 49.7%, full-width at half-maximum <17 nm) and the time to reach 50% photoluminescence of 14,193 h under ambient conditions, showcasing good stability against water immersion (> 3300 h), ultraviolet irradiation, high temperatures (up to 250 °C) and pressure surge (up to 30 MPa). The waterproof PLTs withstood fierce water scouring without any detectable leaching of lead ions. These low-cost and scalable woven PLTs enable breakthrough application in marine rescue.
Universal scaling laws for charge-carrier interactions with quantum confinement in lead-halide perovskites
Authors: Tamarat Philippe , Prin Elise , Berezovska Yuliia , Moskalenko Anastasiia , Nguyen Thi Phuc Tan , Xia Chenghui , Hou Lei , Trebbia Jean-Baptiste , Zacharias Marios , Pedesseau Laurent , Katan Claudine , Bodnarchuk Maryna I. , Kovalenko Maksym V. , Even Jacky , Lounis Brahim 
Published: January 2023

AbstractLead halide perovskites open great prospects for optoelectronics and a wealth of potential applications in quantum optical and spin-based technologies. Precise knowledge of the fundamental optical and spin properties of charge-carrier complexes at the origin of their luminescence is crucial in view of the development of these applications. On nearly bulk Cesium-Lead-Bromide single perovskite nanocrystals, which are the test bench materials for next-generation devices as well as theoretical modeling, we perform low temperature magneto-optical spectroscopy to reveal their entire band-edge exciton fine structure and charge-complex binding energies. We demonstrate that the ground exciton state is dark and lays several millielectronvolts below the lowest bright exciton sublevels, which settles the debate on the bright-dark exciton level ordering in these materials. More importantly, combining these results with spectroscopic measurements on various perovskite nanocrystal compounds, we show evidence for universal scaling laws relating the exciton fine structure splitting, the trion and biexciton binding energies to the band-edge exciton energy in lead-halide perovskite nanostructures, regardless of their chemical composition. These scaling laws solely based on quantum confinement effects and dimensionless energies offer a general predictive picture for the interaction energies within charge-carrier complexes photo-generated in these emerging semiconductor nanostructures.
Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation
Authors: Liu Xinwei , Ye Yingying , Zhu Liling , Xiao Xiaoyun , Zhou Boxuan , Gu Yuanting , Si Hang , Liang Huixin , Liu Mingzhu , Li Jiaqian , Jiang Qiongchao , Li Jiang , Yu Shubin , Ma Ruiying , Su Shicheng , Liao Jian-You , Zhao Qiyi 
Published: January 2023

AbstractEmerging evidence shows that the biomechanical environment is required to support cancer stem cells (CSCs), which play a crucial role in drug resistance. However, how mechanotransduction signals regulate CSCs and its clinical significance has remained unclear. Using clinical-practice ultrasound elastography for patients’ lesions and atomic force microscopy for surgical samples, we reveal that increased matrix stiffness is associated with poor responses to neoadjuvant chemotherapy, worse prognosis, and CSC enrichment in patients with breast cancer. Mechanically, TAZ activated by biomechanics enhances CSC properties via phase separation with NANOG. TAZ-NANOG phase separation, which is dependent on acidic residues in the N-terminal activation domain of NANOG, promotes the transcription of SOX2 and OCT4. Therapeutically, targeting NANOG or TAZ reduces CSCs and enhances the chemosensitivity in vivo. Collectively, this study demonstrated that the phase separation of a pluripotency transcription factor links mechanical cues in the niche to the fate of CSCs.
Massively parallel identification of mRNA localization elements in primary cortical neurons
Authors: Mendonsa Samantha , von Kügelgen Nicolai , Dantsuji Sayaka , Ron Maya , Breimann Laura , Baranovskii Artem , Lödige Inga , Kirchner Marieluise , Fischer Meret , Zerna Nadja , Bujanic Lucija , Mertins Philipp , Ulitsky Igor , Chekulaeva Marina 
Published: January 2023

AbstractCells adopt highly polarized shapes and form distinct subcellular compartments in many cases due to the localization of many mRNAs to specific areas, where they are translated into proteins with local functions. This mRNA localization is mediated by specificcis-regulatory elements in mRNAs, commonly called ‘zipcodes’. Although there are hundreds of localized mRNAs, only a few zipcodes have been characterized. Here we describe a novel neuronal zipcode identification protocol (N-zip) that can identify zipcodes across hundreds of 3′ untranslated regions. This approach combines a method of separating the principal subcellular compartments of neurons—cell bodies and neurites—with a massively parallel reporter assay. N-zip identifies the let-7 binding site and (AU)nmotif as de novo zipcodes in mouse primary cortical neurons. Our analysis also provides, to our knowledge, the first demonstration of an miRNA affecting mRNA localization and suggests a strategy for detecting many more zipcodes.
Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition
Authors: Liu Yusheng , Zhao Han , Shao Fanghong , Zhang Yiwei , Nie Hu , Zhang Jingye , Li Cheng , Hou Zhenzhen , Chen Zi-Jiang , Wang Jiaqiang , Zhou Bing , Wu Keliang , Lu Falong 
Published: January 2023

AbstractPoly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing is known about poly(A) tail dynamics during the human OET. Here, we show that poly(A) tail length and internal non-A residues are highly dynamic during the human OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, maternal mRNAs undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they are re-polyadenylated to produce polyadenylated degradation intermediates, coinciding with massive incorporation of non-A residues, particularly internal long consecutive U residues, into the newly synthesized poly(A) tails. Moreover, TUT4 and TUT7 contribute to the incorporation of these U residues, BTG4-mediated deadenylation produces substrates for maternal mRNA re-polyadenylation, and TENT4A and TENT4B incorporate internal G residues. The maternal mRNA remodeling is further confirmed using PAIso-seq2. Importantly, maternal mRNA remodeling is essential for the first cleavage of human embryos. Together, these findings broaden our understanding of the post-transcriptional regulation of maternal mRNAs during the human OET.