bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bax/Bcl-2 Expression Ratio Analysis of Rat Ovary Vitrified with Date Juice Concentrate as a Natural Extracellular Cryoprotectant
Authors: Safrina Aisyah , Anita Nova , Jusuf Ahmad Aulia , Syaidah Rahimi , Saoemi Huriyah Adani 
Published: July 2023

Background:
The use of extremely low temperatures in vitrification is known to cause cryoinjury so that it can trigger the activation of the intrinsic apoptotic pathway, which can damage the structural integrity of the pre-antral follicle. Based on that, it is necessary to use an appropriate cryoprotectant to protect the preserved cell.

Aims:
This study aimed to identify the potential use of date juice concentrate (DJC) as a natural extracellular cryoprotectant to suppress the rate of apoptosis after vitrification.

Settings and Design:
This experimental research uses 24 samples of ovarian rats. Rats were fed and drank an ad libitum.

Materials and Methods:
Ovaries were isolated in the proestrus phase, then processed into slides for immunohistochemistry (IHC) staining using anti-Bax and anti-Bcl-2 antibodies. IHC results were evaluated for the brown colour using ImageJ IHC Profiler. The results were analysed as an optical density and displayed in the Bax/Bcl-2 ratio.

Statistical Analysis Used:
All data were statistically analysed with either parametric (analysis of various) or non-parametric (Kruskal–Wallis) tests.

Results:
The combination of EG 7.5% + DJC 15% (KP2) showed the lowest Bax/Bcl-2 ratio in primordial and primary follicles. Meanwhile, the lowest Bax/Bcl-2 ratio in secondary follicles is found in KP4 (EG 15% + DJC 15%). The DJC is known to contain a dominant amount of glucose. The DJC shows antioxidant activity and contains antioxidant compounds, phenols and flavonoids.

Conclusion:
The sugar content and antioxidant compounds of DJC can protect against follicle membrane damage, so the rate of intrinsic apoptosis pathway is also suppressed initially with Bax protein suppression in the mitochondrial membrane.
Clinical Outcomes in Patients Receiving Originator Follitropin Alfa and Follitropin Alfa Biosimilars in Real-world Clinical Practice: A Retrospective Study
Authors: Patel Nayana Hitesh , Patel Niket H. , Patel Molina Niket , Bhadarka Harsha K. , Vyas Kairavi Sunilkumar 
Published: July 2023

Background:
Biosimilar drugs have broadened the treatment options in assisted reproductive technology (ART). Real-world data comparing clinical outcomes of originator follitropin alfa (Gonal-f®) with its biosimilars are required to enrich the body of evidence for clinical decision-making on choice of drug.

Aims:
To compare the ART outcomes in patients receiving originator follitropin (Gonal-f®) and its biosimilars in clinical setting.

Settings and Design:
Medical records of 364 infertile women who underwent ART between 2016 and 2020 at Akanksha Hospital and Research Institute, Gujrat, India, were retrospectively analysed.

Materials and Methods:
Participants were divided into two cohorts based on treatment (Gonal-f® cohort; N = 174 and biosimilar cohort; N = 190), each cohort further subdivided into group A (age <35 years) and group B (age ≥35 years). Fresh or frozen embryo transfer was performed as per the standard procedures of the clinic. Pregnancy rates and live birth rate (LBR) were the primary main outcome measures in this study.

Statistical Analysis Used:
Descriptive statistics and Chi-square test were used for analysis.

Results:
The number of oocytes retrieved from Gonal-f® and biosimilar cohorts were comparable (13.3 vs. 14.4). Compared to biosimilars, Gonal-f® treatment resulted in higher yield of cleavage stage and blastocyst stage embryos, and the proportion of women with good quality embryos was higher in the Gonal-f® cohort than the biosimilar cohort (83.3% vs. 69.5%). Patients receiving Gonal-f® reported higher pregnancy rates (59.2% vs. 39.7%) and LBR (43% vs. 17.7%) compared to those receiving biosimilars.

Conclusions:
Gonal-f® (originator follitropin) treatment could result in higher pregnancy rates and LBR in comparison to biosimilars in real-world setting.
Protective Effect of Commercial Grade Vitamin C against Alcohol-induced Testicular Damage in Male Wistar Rats
Authors: George Taiwo Emmanuel , Okhiai Peter Okhemukhokho , Osonuga Ifabunmi Oduyemi , Oyesola Olusoji Adebusoye 
Published: July 2023

Background:
Alcohol consumption has a negative effect on male fertility, but Vitamin C may be able to alleviate this effect.

Aims:
In this study, the protective effect of Vitamin C against alcohol-induced testicular damage in adult male Wistar rats was evaluated.

Settings and Design:
This study was conducted in a University setting. Following a 14-day acclimatisation period, forty adult male Wistar rats were randomly divided into eight groups of five rats. The control group received only food and water, test group B received alcohol only, test group C to E received different doses of Vitamin C, test group F to G received different doses of Vitamin C and alcohol.

Materials and Methods:
After a 21-day treatment period, the testis were harvested and analysed for sperm parameters, antioxidant enzyme activity, level of lipid peroxidation and histopathological changes.

Statistical Analysis Used:
All analyses was performed using SPSS (version 16) and Microsoft Excel (2019) using Student's t-test.

Results:
The results showed that in groups administered with alcohol only, there was a decrease in sperm count. Sperm motility, morphology, viability and antioxidant enzyme activity, but increase in the level of lipid peroxidation. In groups treated with Vitamin C and alcohol, there was improvement in the sperm parameters, antioxidant enzymes activity and a decrease and decrease in lipid peroxidation. Furthermore, in the histology of the testis, regenerative changes were seen.

Conclusion:
The chronic consumption of alcohol can have a deleterious effect on the testis, but commercial-grade Vitamin C can reverse these effects.
Effect of Coronavirus Disease 2019 Pandemic on Spinal Surgery Patients: Our Experience at a Tertiary Care Center
Authors: Verma Virendra K. , Mishra Pankaj K. , Agrawal Udit , Santoshi John A. 
Published: July 2023

ABSTRACT

Introduction:
In December 2019, a cluster of atypical cases of pneumonia was reported in Wuhan, China, which was later designated as Coronavirus disease 2019 (COVID-19) by the World Health Organization (WHO) on Feb 11, 2020. We all are facing a global pandemic, and it is very important to be clear that there is no correct roadmap to navigate this difficult situation. It is imperative to state that this global pandemic impacted the spine care services of our institute. In the present study, we have assessed the spine surgeries performed by orthopedic surgeons in terms of volume and etiologies during the COVID-19 pandemic and compared the data with a pre-COVID period.

Materials and Methods:
We retrospectively collected data from all patients who underwent spinal surgeries at our institute under the department of orthopedics from August 20, 2019 to August 20, 2020 (a total of 12 months duration). The data was then divided into two groups—pre-COVID period (August 20, 2019–February 19, 2020—6 months) and during the COVID pandemic (February 20, 2020–August 20, 2021—6 months).

Results:
A total of 140 patients underwent surgery at our institute from August 20, 2019 to August 20, 2020. Of these, 91 patients underwent surgery during the pre-COVID period, and 49 patients underwent surgery during the COVID pandemic. In this devastating phase of the pandemic, our department’s total number of surgeries significantly declined to 46.15%. The routine surgeries performed during the pandemic phase show a steep fall from 59.34% in the pre-COVID period to 10.20%.

Conclusion:
This paper is meant to focus attention on the exorbitant reduction in the operative workflow of the spine patients during the COVID-19 pandemic at a tertiary healthcare institute. It is the need of the hour that orthopedic surgeons maintain equilibrium while providing the best possible treatment to their patients and limiting the spread of the COVID-19 pandemic.
Doppler Ultrasound Assessment of Tumor Vascularity in Locally Advanced Breast Cancer at Diagnosis and following Primary Systemic Chemotherapy
Authors: Patel Sanjaykumar P. , Nimavat Keyur , Patel Pragnesh , Kachhadia Jenish 
Published: July 2023

ABSTRACT

Aim:
The primary goal of this research is to compare the morphological response, as determined by clinical and sonographic examination, to the gold standard, histology, in assessing the effectiveness of major systemic chemotherapy in patients with locally advanced breast cancer (LABC).

Materials and Methods:
During the check and before each example of chemotherapy (FAC schedule), 54 patients with privately progressed, non-metastatic breast disease underwent a comprehensive clinical assessment, ultrasonography of the breast and axillae, and assortment and spooky Doppler appraisal to evaluate response to therapy.

Results:
In 90% of instances, Doppler provided early insight into how a patient might respond to treatment. Disappearance of all vascular signals inside the tumor was shown to be the strongest predictor of full pathological response.

Conclusion:
Patients with LABC may benefit from using color Doppler to determine how well chemotherapy is working.
Comparative Study of Microbial Adhesion on Different Orthodontic Brackets - An In Vivo Study
Authors: Tripathi Vartika , Assudani Neha , Alasmari Abdullah S. , Meher Jasbir , Khan Salman H. , Khan Samir Sahib M.N. 
Published: July 2023

ABSTRACT

Aim:
To study the microbial adhesion on different orthodontic brackets (conventional, ceramic, and self-ligating brackets).

Materials and Methods:
Three types of bracket systems i.e. self-ligating, conventional, and ceramic brackets were used consisting of 10 patients for each bracket system. Out of 30 patients 20 patients will be treated with conventional and ceramic brackets, in which, in one-half of the mouth steel ligature ties are placed and in the other half elastomeric rings would be placed. We collected swabs from the central incisors and first premolars of the both the right and left sides of both the maxillary and mandibular arches. The samples were collected three times from the above-mentioned teeth once prior to the placement of the brackets, the second and third samples after one and three months respectively.

Result:
Significant variations were between the pretreatment and after one and three months of bracket placement in all three groups. Significant increase in the microbial adhesion of aerobic and anaerobic bacteria in conventional bracket form pretreatment to one and three months after bracket placement is seen. Although the colony formed by anaerobic bacteria is more in number in comparison to the aerobic bacteria.

Conclusion:
Our study reveals that the most hygienic bracket is a self-ligating bracket that should be used in patients who have poor oral hygiene. We also found that using steel ligature is more suitable as compared to elastomeric ligature in both conventional and ceramic brackets.
Comparative Evaluation of the Rigidity and Accuracy of Different Elastomeric Impression Materials with Open Tray Implant Level Impression Technique—An In vitro Study
Authors: Aidasani Ashwin N. , Jain Shailesh , Hassan Sahba , Sharma Anu , Shanthi C. , Fere Sandeep 
Published: July 2023

ABSTRACT

Introduction:
An implant-supported prosthesis requires a precise imprint to provide a good fit. It has been suggested that for implant/abutment level impressions, both the indirect (closed tray) and direct (open tray) methods are effective in achieving a passively fitting prosthesis.

Aim:
With an open plate embed level impression technique, this in vitro investigation set out to evaluate three different elastomeric imprint materials for their relative rigidity and precision.

Materials and Methods:
Sixty bespoke trays were created. Part I (rotational opposition assessment) of the investigation included selecting 30 uniquely shaped plates at random and using them to create embed-level open plate engraves. The second part of this investigation (evaluation of rotational discrepancy and vertical inconsistency) used open plate embed level impressions generated with the remaining thirty individualized plates to evaluate how well these materials recreated the patient’s impressions.

Result:
Within the bounds of the current investigation, vinylpolysiloxane had the highest degree of stiffness among the impression materials examined in comparison to polyether and vinylsiloxanether, as seen by its much greater rotational resistance to torquing. A rotational discrepancy was shown to be much lower in polyether open tray implant level impressions compared to vinylpolysiloxane and comparable to vinylsiloxane.

Conclusion:
In this investigation, the firmest impression medium was vinylpolysiloxane, followed by polyether and vinylsiloxanether for taking imprints of implants. Polyether material, however, was more precise than vinylpolysiloxane and vinylsiloxanether.
Analytical Study of Mandible: Prerequisite for Sex Determination
Authors: Shree Bhagya , Soni Sachin , Sharma Sanjay K. , Handge Keshav , Kumar Ashwini , Das Sushant S. , Puri Nidhi 
Published: July 2023

ABSTRACT

Background:
Human skull consists of various bones. One of them is mandible which is quite resistant, tough and shows systemic differences in form between individuals of different sex. It resists putrefaction also. There are characteristic features in the mandible that help us to differentiate sex in case of unknown victims like in mass disasters or in case fragmentary remains of the skeleton are found. Analysis of mandible with regard to its features is of great assistance in the determination of sex.

Materials and Methods:
A total of 80 dry mandible bones were collected. Morphological and morphometric parameters were studied to determine their sex. A total of nine parameters, i.e., three non-metric and six metric parameters were observed for each mandible. Data was collected for each parameter.

Results:
Among 80 dry mandible bones, 55 were males and 25 were females. 81.2% males bones had a square chin whereas, 80% females had a rounded chin. Gonial flare was everted in 89% males and inverted in 68% females.

Conclusion:
Mandible exhibits significant sexual differences. Various morphological and morphometric parameters are essential for sex determination in case of mandible bone.
Comparative Evaluation of Root Canal Transportation and Centering Ability in Curved Mesiobuccal Canals of Mandibular First Molars after Instrumentation with Different File Systems—An In vitro Study
Authors: Langaliya Akshayraj , Kothari Anjali , Buch Aastha , Dave Riya , Shah Jinali , Shah Nishtha 
Published: July 2023

ABSTRACT

Aim:
To assess the canal transportation and centering ability in the mandibular first molars’ curved mesiobuccal canals after instrumentation with file systems XP endo Shaper, self-adjusting File (SAF), Hyflex EDM, Pro Taper NEXT, WaveOne Gold, and K files with the help of Cone-Beam Computed Tomography (CBCT).

Materials and Methods:
Ninety recently extracted mandibular first molars with mesiobuccal roots that had a 25-30 degree canal curvature were assessed. Following preoperative CBCT scans, teeth were allocated randomly to six experimental groups of fifteen. After preparation with XP endo Shaper, SAF, Hyflex EDM, Pro Taper Next, WaveOne Gold, and K files, post-instrumentation scans were taken using same parameters as pre operative scans. At 3 mm, 6 mm, and 9 mm from the apex, samples were assessed for canal transportation and centering ratio.

Results:
Apical transportation was least for XP endo Shaper as compared to other groups except SAF and the results were statistically significant. K file showed the highest apical transportation toward the lateral wall of the root canal. XP endo Shaper was better in maintaining centering ability of middle and apical third of canal and SAF was better in maintaining centering ability of coronal third.

Conclusion:
In root canal preparation, least apical transportation and better centering ability with XP endo Shaper than other file systems compared. K file showed the highest transportation.
Evaluation of Sealing Ability of MTA Flow, Biodentine and Pro-Root MTA to Seal the Furcal Perforation with and without Internal Matrix- An In vitro Study
Authors: Patel Mahendra , Patel Hardik , Kesharani Pooja , Jani Karna , Shah Khyati , Kapadia Utsavi 
Published: July 2023

ABSTRACT

Introduction:
Endodontic mistakes, also known as procedural accidents, are those bad events that occur during treatment, some of which are attributable to a lack of attention to detail and others of which are completely unforeseeable. The second most frequent reason for root canal failure is perforation.

Aims and Objective:
The current in vitro study’s objective was to assess the furcal perforation’s potential to be sealed with and without internal matrix by MTA flow, Biodentine, and pro-root MTA.

Materials and Methods:
60 samples were allocated into six groups at random (10 each). In intact permanent mandibular first and second teeth, furcal perforation sites were made and sealed with various materials. Following perforation sealing, the specimens’ capacity for sealing was evaluated using the dye penetration method.

Result:
The current study’s findings indicated that Group 2 has the least amount of microleakage and Group 5 has the most.

Conclusion:
Biodentine has excellent sealing capabilities and can be utilised to heal furcation perforations with or without internal matrix.
A Study to Assess Anti-Inflammatory Effect of Vitamin D on Gingivitis: A Dose Dependent Randomised Controlled Trial
Authors: Srivastava Rahul , Raza Hadi , Kabra Pooja , Arya Ashtha , Purushothaman Athira , Thomas Pallavi A. 
Published: July 2023

ABSTRACT

Introduction:
Vitamin D deficiency increases gingivitis risk. Studies show that higher vitamin D levels reduce inflammation.

Materials and Methods:
College conducted a double-blind, randomized, controlled trial on 451 people’s vitamin D and gingival health at baseline. For three months, participants were given a placebo (group A), 1000 international units (IU) of vitamin D3 per day (group B), 500 IU per day (group C), or 2000 IU per day (group A). Gingival scores were recorded for three months to assess the anti-inflammatory effect (the first, second, and third). Vitamin D levels also affected gingivitis.

Results:
Vitamin D dose-dependently reduces gingivitis inflammation. Vitamin D administration negatively correlated with gingival index score. Anti-inflammatory vitamin D levels are 32–37 ng/ml.

Conclusion:
Vitamin D supplementation can reduce gingivitis severity. The optimal blood vitamin D level is 30–35 ng/ml.
Incidence of Canine Impaction in Tribal Population of Jharkhand
Authors: Jha Awanindra K. , Chandra Subhash , Mallick Saurabh , Prakash Om , Ranjan Vikrant , Ekram Subia 
Published: July 2023

ABSTRACT

Introduction:
The position of the canine has a key role in facial harmony, dental aesthetics, functional occlusion, and health of temperomandibular joint. Maxillary canine impaction is the second most common tooth after third molars impaction. Maxillary canines are more prone to impaction due to longer path of eruption.

Settings and Design:
All the patients were examined in the Dental Chair light and referred to the department of Oral Radiology for Orthopantomogram. Yamamoto’s classification was used to classify maxillary canine impaction. Position of mandibular canine from cement-enamel junction was used for mandibular canine impaction.

Materials and Methods:
The study was performed on 2300 patients who consulted the orthodontic department from January 2018 to July 2022. Orthopantomogram of each patient was examined for canine impaction, retained deciduous teeth, cyst, and other anomalies. The incidence and pattern of canine impaction was assessed.

Statistical Analysis:
The data was then statistically analyzed using Graph Pad Prism software. The incidence of impacted canine was evaluated. The effect of gender was evaluated using a t-test.

Results:
A total of 2300 subjects were examined out of which 52 individuals were diagnosed with canine impaction. The incidence of canine impaction among orthodontic patients is 2.26%. The amount of canine impaction in females (60%) is higher than males (40%). The ratio of canine impaction of the maxilla and mandible is 2.5.

Conclusion:
The incidence of canine impaction was found to be 2.26 percent in the tribal dominant population of Jharkhand. Type II (50%) pattern in the maxilla and Level A (57%) impaction in the mandible was found to be the highest.
Dermatoglyphic Patterns in Patients Having Impacted and Erupted Third Molars—A Comparative Study
Authors: Mehta Dhaval N. , Thakkar Vallabha C. , Mandviya Parth , Jadav Braina , Goswami Riddhi , Chavda Raksha 
Published: July 2023

ABSTRACT

Background:
The dermatoglyphics have been used in several researches for predicting various pathologies of oral cavity like periodontitis, dental caries, impacted teeth, mal-occlusion, developmental defects such as cleft lip and palate, and potentially malignant disorders. Impacted teeth may be associated with various pathologies such as caries, pericoronitis, dentigerous cysts, and keratocystic odontogenic tumors; therefore, predicting impacted teeth through dermatoglyphic patterns can help in diagnosis and future treatment planning of such event.

Aims and Objectives:
The aim and objective of the study were to evaluate and compare various dermatoglyphic patterns in the patients with the impacted maxillary and/or mandibular third molar (group 1) and patients having erupted maxillary and/or mandibular third molar (group 2).

Materials and Methods:
This study comprised totally 300 patients, which were divided into two groups, according to erupted or impacted maxillary and/or mandibular third molar visiting outpatient department, who fulfilled the selection criteria for the study. After a comprehensive clinical examination, dermatoglyphic patterns were recorded from the same individuals. Panoramic radiographs were taken to confirm the diagnosis. Data collected were entered in the spreadsheet and analyzed. For all tests, P value was set at <0.05 and was considered statistically significant.

Result:
There was statistical significance noted in frequencies of whorl pattern in patients of group 1 and loop pattern in patients of group 2.

Conclusion:
This study concluded that dermatoglyphic patterns can be used to assess the prevalence of impacted wisdom teeth.
A Retrospective Analysis to Assess the Reasons for the Failure of Dental Implants
Authors: Yarramsetty Greeshma Vani , Singiri Bindhu Madhavi , Vijay K. R , Balaji V. C. , Anusha Kaki , Thota Roger P. 
Published: July 2023

ABSTRACT

Background:
To assess the reasons for dental implant failures.

Materials and Methods:
A total of 100 implants were studied in 80 subjects. The radiographic investigations were performed to assess the record length of implant and diameter of implant. Risk factors, such as habit of smoking, history of diabetes, and hypertension, were recorded. The data were collected and assessed using Statistical Package for the Social Sciences (SPSS) software. The one-way analysis of variance (ANOVA) test was used to assess the failure rate in dental implants. A P-value less than 0.05 was considered statistically significant.

Results:
A total of 100 dental implants were included. The maximum dental implant failure was seen with a diameter <3.75 mm (30%) followed by 3.75–4.5 mm (16%) and >4.5 mm (6.15%). The difference was found to be significant (P < 0.05).

Conclusion:
Dental implant failure was high in dental implants with <3.75 mm diameter, dental implants with a length <10.0 mm, and among smokers.