bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detailed Survey of an in-vitro Intestinal Epithelium Model by Single-Cell Transcriptomics
Authors: Ran Ran , Munoz Javier , Jena Smrutiti , Green Leopold N. , Brubaker Douglas K. 
Published: May 2023

AbstractThe gut plays a critical role in maintaining human health by facilitating the absorption of nutrients, regulating metabolism, and interacting with the immune system and gut microbiota. The co-culture of two human colorectal cancer cell lines, Caco-2 and HT29, on Transwell is commonly used as anin vitrogut mimic in studies of intestinal absorption pharmacokinetics, gut mechanics, and gut-microbe interplay given the similar morphology, expression of transporters and enzymes, and barrier function. However, to sufficiently evaluate the translatability of insights from such a system to human physiological contexts, a detailed survey of cell type heterogeneity in the system and a holistic comparison with human physiology are needed to be conducted rather than by the presence of a few well-studied proteins. Single-cell RNA sequencing provides high-resolution expression profiles of cells in the co-culture, enabling the heterogeneity to be characterized and the similarity to human epithelial cells to be evaluated. Transcriptional profiles of 16019 genes in 13784 cells were acquired and compared to human epithelial cells (GSE185224). We identified the intestinal stem cell-, transit amplifying-, enterocyte-, goblet cell-, and enteroendocrine-like cells together with differentiating HT29 cells in the system based on the expression of canonical markers in healthy adult human epithelial cells. The epithelium-like co-culture was fetal intestine-like, with less variety of gene expression compared to the human gut. Transporters for major types of substance (lipid, amino acid, ion, water, etc.) were found transcribed in the majority of the enterocytes-like cells in the system. However, some of the well-studied transporters such as FATP4 and GLUT2 were absent. Toll-like receptors were not highly expressed in the sample, yet the treatment of lipopolysaccharide still caused a mild change in trans-epithelial electrical resistance and gene expression, possibly by the interaction with CD14, the co-receptor for TLRs. Overall, the Caco-2/HT29 co-culture is a cost-effective epithelium model for drug permeability testing or mechanical simulation, but its phenotypic discrepancy with the real epithelium is not negligible. As a result, its response to biological factors might not provide transferrable knowledge to the study of human gut physiology, especially the innate immune aspect.
Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization inPseudomonas putidaM2
Authors: Shrestha Shilva , Awasthi Deepika , Chen Yan , Gin Jennifer , Petzold Christopher J. , Adams Paul D. , Simmons Blake A. , Singer Steven W. 
Published: May 2023

ABSTRACTPseudomonas putidahave emerged as promising biocatalysts for the conversion of sugars and aromatics obtained from lignocellulosic biomass. Understanding the role of carbon catabolite repression (CCR) in these strains is critical to optimize biomass conversion to fuels and chemicals. The CCR functioning inP. putidaM2, a strain capable of consuming both hexose and pentose sugars as well as aromatics, was investigated by cultivation experiments, proteomics, and CRISPRi-based gene repression. Strain M2 co-utilized sugars and aromatics simultaneously; however, during co-cultivation with glucose and phenylpropanoid aromatics (p-coumarate and ferulate), intermediates (4-hydroxybenzoate and vanillate) accumulated, and substrate consumption was incomplete. In contrast, xylose-aromatic consumption resulted in transient intermediate accumulation and complete aromatic consumption, while xylose was incompletely consumed. Proteomics analysis revealed that glucose exerted stronger repression than xylose on the aromatic catabolic proteins. Key glucose (Eda) and xylose (XylX) catabolic proteins were also identified at lower abundance during co-cultivation with aromatics implying simultaneous catabolite repression by sugars and aromatics. Downregulation ofcrcvia CRISPRi led to faster growth and uptake of glucose andp-coumarate in the CRISPRi strains compared to the control while no difference was observed on xylose +p-coumarate. The increased abundance of the Eda and amino acids biosynthesis proteins in the CRISPRi strain further supported these observations. Lastly, small RNAs (sRNAs) sequencing results showed that CrcY and CrcZ homologues levels in M2, previously identified inP. putidastrains, were lower under strong CCR (glucose +p-coumarate) condition compared to when repression was absent (p-coumarate or glucose only).IMPORTANCEA newly isolatedPseudomonas putidastrain,P. putidaM2, can utilize both hexose and pentose sugars as well as aromatics making it a promising host for the valorization of lignocellulosic biomass. Pseudomonads have developed a regulatory strategy, carbon catabolite repression, to control the assimilation of carbon sources in the environment. Carbon catabolite repression may impede the simultaneous and complete metabolism of sugars and aromatics present in lignocellulosic biomass and hinder the development of an efficient industrial biocatalyst. This study provides insight into the cellular physiology and proteome during mixed-substrate utilization inP. putidaM2. The phenotypic and proteomics results demonstrated simultaneous catabolite repression in the sugar-aromatic mixtures while the CRISPRi and sRNA sequencing demonstrated the potential role of thecrcgene and small RNAs in carbon catabolite repression.
Live tracking of a plant pathogen outbreak reveals rapid and successive, multidecade episome reduction
Authors: Roman-Reyna Veronica , Sharma Anuj , Toth Hannah , Konkel Zachary , Omiotek Nicolle , Murthy Shashanka , Faith Seth , Slot Jason , Hand Francesca Peduto , Goss Erica , Jacobs Jonathan M. 
Published: May 2023

AbstractQuickly understanding the genomic changes that lead to pathogen emergence is necessary to launch mitigation efforts and reduce harm. Often the evolutionary events that result in an epidemic typically remain elusive long after an outbreak, which is particularly true for plant pathogens. To rapidly define the consequential evolutionary events result in pathogen emergence, we tracked in real-time a 2022 bacterial plant disease outbreak in US geranium (Pelargoniumxhortorum) caused by Xhp2022, a novel lineage ofXanthomonas hortorum. Genomes from 31 Xhp2022 isolates from seven states showed limited chromosomal variation, and all contained a single plasmid (p93). Time tree and SNP whole genome analysis estimated that Xhp2022 emerged in the early 2020s. Phylogenomic analysis determined that p93 resulted from cointegration of three plasmids (p31, p45, and p66) present in a 2012 outbreak. p31, p45 and p66 were individually found in varying abundance acrossX. hortorumisolates from historical outbreaks dating to 1974 suggesting these plasmids were maintained in the broader metapopulation. p93 specifically arose from two co-integration events from homologous and Tn3and XerC-mediated site-specific recombination. Although p93 suffered a 49kb nucleotide reduction, it maintained critical fitness gene functions encoding, for example, metal resistance and virulence factors, which were likely selected by the ornamental production system. Overall we demonstrate how rapid sequencing of current and historical isolates track the evolutionary history of an emerging, ongoing threat. We show a recent, tractable event of genome reduction for niche adaptation typically observed over millenia in obligate and fastidious pathogens.SignificanceGenome-resolved epidemiology is rapidly changing how we track pathogens in real-time to support stakeholders and health. This research highlights how we responded to a current disease outbreak of geranium. Our work revealed that a new group of the bacterial plant pathogenXanthomonas horotrumemerged in 2022 as a result of a recent genome reduction. We determined that three distinct plasmids were present in the broaderX. hortorummetapopulation since 1974. In 2012, the three plasmids were altogether present in individual isolates; then in 2022, all three plasmids co-integrated while maintaining critical fitness genes but losing extraneous genomic material. This parallels genome efficiency and reduction that we see across millenia or even millions of years with obligate parasites with increased niche-specificity.
Effects of the cementite morphology on the hydrogen trapping behavior in the pipeline steel
Authors: Tu Yanqi , Liu Saiyu , Shi Rongjian , Yang Shani , Gao Kewei , Pang Xiaolu 
Published: May 2023

Purpose
The purpose of this study is to investigate the effects of the cementite morphology on the hydrogen trapping behavior in low-alloy pipeline steel.

Design/methodology/approach
In this study, the hydrogen trapping behavior in low-alloy pipeline steel was quantitatively studied by a combination of microstructural observations, electrochemical hydrogen permeation experiments and thermal desorption spectroscopy (TDS) analyses.

Findings
P-1 and P-2 steels are two samples with different microstructures. The morphology of cementite precipitates in the P-1 and P-2 steels was different. Lamellar cementite is present in P-2 steel and only granular cementite in P-1 steel, which led to a better irreversible hydrogen trapping ability of P-2 steel, which was confirmed by subsequent hydrogen permeation and TDS experiments.

Originality/value
The study of these deep hydrogen trap sites is helpful in improving the hydrogen embrittlement resistance of low-alloy pipeline steels.
Blockchain: a promising technology for judicial translation services in cases with foreign elements
Authors: Huang Lingling , Zhao Chengqiang , Chen Shijie , Zeng Liujing 
Published: May 2023

PurposeTechnical advantages embraced by blockchain, such as distributed ledger, P2P networks, consensus mechanisms and smart contracts, are highly compatible with addressing the security issues of transferring and storing judicial documents and obtaining the feedback and evaluation of judicial translation services in cases with foreign elements. Therefore, based on this, a consortium blockchain-based model for supervising the overall process of judicial translation services in cases with foreign elements is proposed.Design/methodology/approachSome judicial documents are required to be translated when there are language barriers in cases with foreign elements. The purpose of this paper is expected to address security issues, which is ignored, in the process of translating judicial documents.FindingsThe experimental results show that the model constructed in this paper can effectively guarantee the security and privacy of transferring and storing translated judicial documents in cases with foreign elements, and realize the credibility and traceability of feedbacks and evaluations of judicial translation services. In addition, the underlying network communications is stable and the speed for processing data can meet the requirements of practical application.Originality/valueThe research in this paper provides an innovative scheme for judicial translation services in cases with foreign elements. The model constructed is conducive to protecting the security of the transfer and storage of judicial documents and improving the efficiency and modernization ability of hearing cases with foreign elements.
How price labeling strategy affects consumers' purchase intention? The role of perceived price difference in price assessment
Authors: Liang Wanqi , Zhou Deyi , Rizwan Muhammad , Huseynov Samir 
Published: May 2023

PurposeBy conducting an online experiment, this paper proposes and tests a conceptual model about the impact of price labeling strategy on consumers' perceived price difference and purchase intention. The authors also analyze differential influences of shopping channels and price levels on documented effects. The paper provides strategic suggestions for online grocery store managers to adopt profit-maximizing labeling decisions.Design/methodology/approachIn a between-subject experiment, the authors simulated a shopping task with eight scenarios by exogenously manipulating price labeling strategies (unit price/retail price), sales channels (online/offline) and price levels (higher/lower than the average price). Participants are randomly assigned to one of the eight scenarios and asked to report their perceived price difference between the stimuli product and the average market price and their purchase intention on the stimuli product.FindingsExperimental results show that compared to the unit price, the retail price increases the perceived price difference. It shows that the unit price increases consumers' purchase intention when the product price is higher than the average market price. However, these effects only exist in the online shopping context.Originality/valueThis paper extends the study of price labeling strategy to an online shopping context and examines the mediation effect of the perceived price difference.
Integrating lean construction with BIM and sustainability: a comparative study of challenges, enablers, techniques, and benefits
Authors: Moradi Sina , Sormunen Piia 
Published: May 2023

Purpose
The construction industry has considerably evolved in the recent two decades due to the emergence of sustainability, lean construction (LC) and building information modelling (BIM). Despite previous research efforts, there is still a gap concerning the multidimensional nature of their integration. Hence, this study aims to fill the mentioned knowledge gap through exploring and comparing the challenges, enablers, techniques as well as benefits of integrating LC with BIM and sustainability in building construction projects.

Design/methodology/approach
A systematic literature review was conducted to fulfill the purpose of this study.

Findings
The findings reveal and compare the challenges, enablers, techniques and benefits of integrating LC with BIM and sustainability in building construction projects. The results suggest that there are eight common challenges for integrating LC with BIM and sustainability, including high initial cost, lack of collaboration, lack of professionals and lack of compatible contractual framework. The discovered challenges, enablers, techniques and benefits seem to be mostly routed in people. The findings also suggest that the synergistic benefits of integrating LC with BIM and sustainability can overcome the common challenges (safety, reliability, productivity, collaboration and quality) in construction projects.

Originality/value
The findings contribute to the literature and practice concerning the integration of LC with BIM and sustainability by exploring, comparing and discussing the relevant challenges, enablers, techniques as well as benefits. Moreover, the findings reveal the significance of the development of people in construction industry, besides processes and technology, as people are always subject of activities in construction while processes and technology are always objects.
The effects of joint-contract functions on PPP project value creation: a mediation model
Authors: Li Xuewei , Yuan Jingfeng , Liu Xuan , Wang Guangqi , Wang Qian-Cheng 
Published: May 2023

PurposeWith the continuous improvement of public–private partnership (PPP) projects, the participants' value creation goals are not only limited to achieving the basic performance objectives but also to realising value added. However, the effect of traditional contract management on realising the value creation objectives of PPP projects is limited. According to the view of multifunctional contract, joint-contract functions that integrate contract control and flexibility are likely to be effective in enhancing the value creation of PPP projects. This study aims to explore the effects of joint-contract functions on PPP project value creation and relevant influencing mechanism by investigating the mediating effect of in-role behaviour and extra-role behaviour.Design/methodology/approachAfter collecting 258 valid questionnaires from PPP professionals in China, this study used structural equation modelling to validate the hypotheses.FindingsContract control and flexibility can improve PPP project value creation. Specifically, contract control improves the achievement of the basic contract objectives of PPP projects, whereas contract flexibility enhances the achievement of the value-added of PPP projects. Moreover, only in-role behaviour mediates the effect of contract control on value creation. In addition, the mediating effect of extra-role behaviour on the impact of contract flexibility on value creation is stronger than that of in-role behaviour. The mediating effect of in- and extra-role behaviour is mainly reflected in the realisation of basic and value-added performance, respectively.Research implicationsThe findings of this study can help realise value creation in three ways. Firstly, new perspectives for PPP project value creation should be proposed by combining the improvement of contract objectives and the realisation of the participants' implicit demands. Secondly, the effects of different contract functions on value creation should be analysed instead of a single dimension of contractual governance. Thirdly, the mediating effects of different types of cooperation behaviour that may influence the relationship between contractual governance and value creation should be evaluated.Originality/valueThis study verifies the impacts of different contract functions on PPP project value creation. In addition, cooperative behaviour is embedded as a mediating variable, and the mediated transmission path from contract function to cooperative behaviour and further to PPP project value creation is systematically analysed.
Development of Gulf Cooperation Council human resources: an evidence-based review of workforce nationalization
Authors: Elbanna Said , Obeidat Shatha M. , Younis Heba , Elsharnouby Tamer H. 
Published: May 2023

PurposeThis study aimed to contribute to the field of Human Resource Management (HRM) by providing a critical review of existing scholarly research and a thematic analysis of the workforce nationalization domain in the Gulf Cooperation Council (GCC) countries. To strengthen the literature on this topic, it seeks to identify key gaps and areas for further exploration.Design/methodology/approachA two-step systematic research methodology (qualitative and quantitative) and a thematic analysis of empirical and theoretical studies were used in this study. The quantitative review was conducted using a predesigned coding framework.FindingsThe study identified and discussed four perspectives of workforce nationalization in the GCC countries. These were (1) the conceptualization of workforce nationalization; (2) the role of institutional policies in achieving it; (3) the practices and outcomes of nationalization efforts and (4) the impact of gender and women in the nationalization process.Research limitations/implicationsThis study has several limitations, which the authors have addressed by proposing several future research avenues. For example, the reviewed studies are skewed toward certain countries (e.g. UAE and Saudi Arabia), which limits the generalizability of their findings.Practical implicationsA more comprehensive definition of nationalization, development of qualitative and quantitative measures to enhance HRM practices and outcomes, and the identification of alternative approaches to improve the employment of locals are emphasized as needs. Additionally, revised measures and mechanisms to rectify negative perceptions about entitlement and the revision of policies to integrate females in the national labor force are suggested.Originality/valueWorkforce nationalization initiatives in the GCC region offer a unique and rich research phenomenon replete with managerial, organizational, economic and political dilemmas. The investigation of this phenomenon would profoundly enlighten employers, policymakers and scholars.
Inhomogeneous waves propagation in double-porosity thermoelastic media
Authors: Kumar Manjeet , Bhagwan Jai , Kaswan Pradeep , Liu Xu , Kumari Manjeet 
Published: May 2023

Purpose
The purpose of this study is to investigate the reflection of plane waves in a double-porosity (DP) thermoelastic medium.

Design/methodology/approach
To derive the theoretical formulas for elastic wave propagation velocities through the potential decomposition of wave-governing equations. The boundary conditions have been designed to incorporate the unique characteristics of the surface pores, whether they are open or sealed. This approach provides a more accurate and realistic mathematical interpretation of the situation that would be encountered in the field. The reflection coefficients are obtained through a linear system of equations, which is solved using the Gauss elimination method.

Findings
The solutions obtained from the governing equations reveal the presence of five inhomogeneous plane waves, consisting of four coupled longitudinal waves and a single transverse wave. The energy ratios of reflected waves are determined for both open and sealed pores on the stress-free, the thermally insulated surface of DP thermoelastic medium. In addition, the energy ratios are compared for the cases of a DP medium and a DP thermoelastic medium.

Originality/value
A numerical example is considered to investigate the effect of fluid type in inclusions, temperature and inhomogeneity on phase velocities and attenuation coefficients as a function of frequency. Finally, a sensitivity analysis is performed graphically to observe the effect of the various parameters on propagation characteristics, such as propagation/attenuation directions, phase shifts and energy ratios as a function of incident direction in double-porosity thermoelasticity medium.
On the analysis of thermosolutal mixed convection in differentially heated and soluted geometries beyond rectangular
Authors: Hansda Samrat , Pandit Swapan K. 
Published: May 2023

Purpose
This paper aims to study the impact of convexity and concavity of the vertical borders on double-diffusive mixed convection. In addition, the study of entropy generation is performed. This numerical study has been carried out for different patterns of wavy edges to reveal their effects on heat and mass transfer phenomena.

Design/methodology/approach
Four different flow features are treated by varying the directions of convexity and concavity of the vertical walls. A uniform temperature, as well as concentration distributions, are introduced to the left border while keeping a cold temperature and low concentration for the right border. The horizontal boundaries are in adiabatic condition. The upper border of the chamber is moving in the right direction with an equal speed. The governing Navies–Stokes equations are designed to describe energy and species transport phenomena, and these equations are solved by compact scheme.

Findings
The investigated results are analyzed for various parameters, namely, Prandtl number, Richardson number, thermal Grashof number, Lewis number, Buoyancy ratio and amplitude of the wavy walls. It is observed that the thermal and solutal transfer performance becomes effective with lower Richardson numbers. The results reveal that the concavity and convexity of the side borders of the cabinet can control the thermosolutal performance. It is also observed that among all wavy chambers, Case-4 records maximum thermosolutal transfer rate, while Case-3 attains minimum thermosolutal transfer rate.

Originality/value
This work is an example of solar thermal power conversion, power collection systems, systems of energy deficiency, etc.