bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nursing Care of HIV-Positive Women: A Scoping Literature Review on the Emotional Challenges for Women Living with a Chronic Disease
Authors: Nielsen Ben Farid Røjgaard , Martinsen Bente 
Published: August 2023

AbstractLiving with HIV seems to be more stressful for women than men. Women exhibit higher levels of depression, lower levels of well-being and lower quality of life than HIV-infected men do. The aim of the review is to provide a comprehensive gender specific knowledge about the emotional challenges HIV infected women face in their daily lives. Across all articles one theme that emerged was the challenges about the social environment after being diagnosed with HIV. Three identified show what challenges women with HIV are facing in relation to employment, in relation to other people and a fear of stigma. Another theme that emerged was the challenges of different coping mechanisms. There was an evolution in how an HIV diagnosis had affected women’s lives and their ability to cope with their diagnoses. Three identified subthemes show what challenges women with HIV are facing in relation to aiming to improve quality of life after being diagnosed with HIV, a sharp distinction between HIV and AIDS and a religious and spiritually coping. Identifying the emotional challenges women living with HIV face in their daily lives may help nurses obtain a clearer understanding and greater knowledge of how to provide HIV-positive women with effective care that empower and support these women in managing their chronic disease. However to ensure that nurses have the proper tools for effective care for women living with HIV European studies are essentials in relation to what emotional challenges these women are facing in their daily lives.
Impact of Micro Health Insurance Plans on Protecting Households Against Catastrophic Health Spending in Tanzania
Authors: Kihaule Arnold 
Published: August 2023

AbstractThe paper analyses the impact of households’ membership in micro health insurance plans on the protection against catastrophic health spending, in the episodes of illness. The analysis considers the groups of poor and non-poor members of micro health insurance schemes and non-members in both urban and rural areas in Tanzania.Matching estimator method was used to analyse the effect of membership in the micro health insurance on the protection against catastrophic health spending, in the episodes of illness among the members of micro health insurance scheme and non-members for the different income groups. Data used in the analysis involved those on the 10,300 households involved in Tanzania Demographic and Health Survey of 2011.The estimation results revealed that insurance schemes enabled households to get protection against catastrophic health spending, in the episodes of illness to members of insurance schemes compared to non-members. Thus, it is recommended that households be encouraged to enroll in the micro health insurance schemes. The insurance scheme also needs to be close to the would be customers. This will also contribute to poverty reduction in the country as well.
A Model worth Sharing: A Community Mental Health Clinic with an Award Winning Integrated Physical Health Program
Authors: Wallace Elizabeth 
Published: August 2023

AbstractThe lifespan of people with severe mental illness (SMI) is shorter compared to the general population. This excess mortality is mainly due to physical illness. (De Hert et al 2011). Mental health services should be able to provide at least a standard routine assessment of their patients, in order to identify or suspect the presence of physical health problems. (Maj, M 2009)The first step in addressing the gap between psychiatric and health management is to integrate physical health into mental health planning. (Miller, H 2008)The paper provides an overview of the innovative nurse led Physical Health Program which has successfully integrated into the core business of a community mental health clinic within Melbourne’s public health service and created working partnerships with primary care and community health.The Program received recognition as Gold Winner in the Excellence in Person Centred Care category at the Victorian Public Health Awards in 2014 and has been in place for three years, providing physical health screening, signposting and individual evidence based interventions to consumers with severe mental illness.Dedicated nursing roles and the development of a unique approach provides a barrier free long term care model which has returned high rates of engagement with consumers in addressing their physical health needs and goals.The model, which has been promoted for replication across the service, showcases best practice, the value of collaborative working with our community health and primary care partners, and the substantial progress and effective outcomes that can be achieved when a mental health nurses takes the lead in innovation and change.
Low-dose lipopolysaccharide inhibits spinal cord injury-induced neuronal apoptosis by regulating autophagy through the lncRNA MALAT1/Nrf2 axis
Authors: Hu Jianhua , Huang Kun , Bao Feilong , Zhong Shixiao , Fan Qianbo , Li Weichao 
Published: August 2023

Background
Spinal cord injury (SCI) is a neurological disease associated with a high disability rate. Low-dose lipopolysaccharide (LPS) has been reported to activate cross-immune tolerance and alleviate the effects of various traumatic stimuli. The present study aimed to explore the effect of LPS on SCI and the potential molecular mechanism.

Methods
Male Sprague-Dawley (SD) rats were used to established an in vivo SCI model and were intraperitoneally injected with lentivirus particles encoding a MALAT1 small interfering RNA (siRNA) on day 10 prior to SCI and with 0.2 mg/kg LPS 72 h prior to SCI. Basso, Beattie, and Bresnahan (BBB) scoring; HE staining; and TUNEL assay were used to assess neurological function and pathophysiological changes. Western blot and immunohistochemistry (IHC) were used to detect cell autophagy and Nrf2 nuclear translocation. PC12 cells were exposed to oxygen-glucose deprivation/reoxygenation (OGD/R) to establish an in vitro SCI model. In vitro SCI model cells were pretreated with LPS and transfected with siMALAT1 or MALAT1 overexpression plasmid aimed at knocking down MALAT1 or overexpressing MALAT1. The cell counting kit-8 (CCK-8) assay was used to measure the toxicity of LPS towards PC12 cells. Flow cytometry and immunofluorescence analysis were performed to investigate cell apoptosis and Nrf2 nuclear translocation.

Results
SCI rats preconditioned with low-dose LPS had higher BBB scores, reduced SCI injury, increased MALAT1 expression and activated autophagy and Nrf2 nuclear translocation in the in vivo SCI model. In the in vitro SCI model, low-dose LPS treatment suppressed the apoptotic ratio of PC12 cells, increased MALAT1 expression, activated autophagy, and promoted Nrf2 nuclear translocation. Silencing MALAT1 exacerbated OGD/R injury in vitro and weakened the protective effect of low-dose LPS. Overexpression of MALAT1 inhibits OGD/R-induced apoptosis by inducing autophagy and promoting Nrf2 nuclear translocation. This was also been confirmed in animal experiments, silencing MALAT1 blocked the promotion of Nrf2 by low-dose LPS and the alleviated of SCI apoptosis.

Conclusions
Low-dose LPS exhibited a protective role on SCI by activating autophagy and suppressing nerve cell apoptosis via the lncRNA MALAT1/Nrf2 axis.
Identification of Zip8-correlated hub genes in pulmonary hypertension by informatic analysis
Authors: Zhao FanRong , Chen Yujing , Xie Yuliang , Kong Shuang , Song LiaoFan , Li Hanfei , Guo Chao , Yin Yanyan , Zhang Weifang , Zhu Tiantian 
Published: August 2023

Background
Pulmonary hypertension (PH) is a syndrome characterized by marked remodeling of the pulmonary vasculature and increased pulmonary vascular resistance, ultimately leading to right heart failure and even death. The localization of Zrt/Irt-like Protein 8 (ZIP8, a metal ion transporter, encoded by SLC39A8) was abundantly in microvasculature endothelium and its pivotal role in the lung has been demonstrated. However, the role of Zip8 in PH remains unclear.

Methods
Bioinformatics analysis was employed to identify SLC39A8 expression patterns and differentially expressed genes (DEGs) between PH patients and normal controls (NC), based on four datasets (GSE24988, GSE113439, GSE117261, and GSE15197) from the Biotechnology Gene Expression Omnibus (NCBI GEO) database. Gene set enrichment analysis (GSEA) was performed to analyze signaling pathways enriched for DEGs. Hub genes were identified by cytoHubba analysis in Cytoscape. Reverse transcriptase-polymerase chain reaction was used to validate SLC39A8 and its correlated metabolic DEGs expression in PH (SU5416/Hypoxia) mice.

Results
SLC39A8 expression was downregulated in PH patients, and this expression pattern was validated in PH (SU5416/Hypoxia) mouse lung tissue. SLC39A8-correlated genes were mainly enriched in the metabolic pathways. Within these SLC39A8-correlated genes, 202 SLC39A8-correlated metabolic genes were screened out, and seven genes were identified as SLC39A8-correlated metabolic hub genes. The expression patterns of hub genes were analyzed between PH patients and controls and further validated in PH mice. Finally, four genes (Fasn, Nsdhl, Acat2, and Acly) were downregulated in PH mice. However, there were no significant differences in the expression of the other three hub genes between PH mice and controls. Of the four genes, Fasn and Acly are key enzymes in fatty acids synthesis, Nsdhl is involved in cholesterol synthesis, and Acat2 is implicated in cholesterol metabolic transformation. Taken together, these results provide novel insight into the role of Zip8 in PH.
Comprehensive transcriptional analysis of pig facial skin development
Authors: Li Yujing , Shi Rui , Yuan Rong , Jiang Yanzhi 
Published: August 2023

BackgroundSkin development is a complex process that is influenced by many factors. Pig skin is used as an ideal material for xenografts because it is more anatomically and physiologically similar to human skin. It has been shown that the skin development of different pig breeds is different, and some Chinese pig breeds have the characteristics of skin thickness and facial skin folds, but the specific regulatory mechanism of this skin development is not yet clear.MethodsIn this study, the facial skin of Chenghua sows in the four developmental stages of postnatal Day 3 (D3) , Day 90 (D90) , Day 180 (D180), and Year 3 (Y3) were used as experimental materials, and RNA sequencing (RNA–seq) analysis was used to explore the changes in RNA expression in skin development at the four developmental stages, determine the differentially expressed messenger RNAs (mRNAs), long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs), and perform functional analysis of related genes by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.ResultsA pairwise comparison of the four developmental stages identified several differentially expressed genes (DEGs) and found that the number of differentially expressed RNAs (DE RNAs) increased with increasing developmental time intervals. Elastin (ELN) is an important component of the skin. Its content affects the relaxation of the epidermis and dermal connection, and its expression is continuously downregulated during the four developmental stages. The functions of DEGs at different developmental stages were examined by performing GO and KEGG analyses, and the GO terms and enrichment pathways of mRNAs, lncRNAs, miRNAs, and circRNAs highly overlapped, among which the PPAR signaling pathway, a classical pathway for skin development, was enriched by DEGs of D3 vs. D180, D90 vs. D180 and D180 vs. Y3. In addition, we constructed lncRNA-miRNA-mRNA and circRNA-miRNA interaction networks and found genes that may be associated with skin development, but their interactions need further study.ConclusionsWe identified a number of genes associated with skin development, performed functional analyses on some important DEGs and constructed interaction networks that facilitate further studies of skin development.
Social Change in the Kampung Sejahtera Village of Medan City, Indonesia
Authors: Saputra Agung , Ibrahim Jabal Tarik , Sukmana Oman , Rudianto  
Published: August 2023

Aims: This study aims to analyze the social changes that have taken place in Kampung Sejahtera, Medan City, Indonesia. This social change was motivated by the community's desire to change the bad stigma attached to this area which is synonymous with drug trafficking, prostitution, gambling and other criminal acts into a village that is safe, comfortable, beautiful, prosperous and livable.
Place and Duration of Study: This study was conducted in Kampung Sejahtera, Medan City, Indonesia. The research started from May 2022 to July 2023
Methodology: This study uses a qualitative approach. This qualitative approach was chosen to find out how social changes have occurred in the people living in the Prosperous Village of Medan City. The type of study used in this study is a case study. In simple terms, the case study in this study is defined as a process of in-depth and detailed investigation or examination of social change events that occurred in the Prosperous Village of Medan City.
Results: Social change in Kampung Sejahtera, Medan City, Indonesia occurs through demographic processes, economic processes, ideas and social movements that have been going on for a long time.
Conclusion: The social change that occurred in Kampung Sejahtera, Medan City was motivated by the people's desire to change the reputation of Kampung Kubur as a slum village and drug den inhabited by criminals to become a Prosperous Village that is safe, comfortable and conducive. The social changes that occurred in Kampung Sejahtera were generally initiated by figures from the community, government figures and the Police. The social changes that have occurred have changed the social structure that has occurred for the better. This can be seen in the reduction in drug trafficking and abuse, the creation of a better, beautiful and comfortable social environment as well as changes in the outside community's assessment of the bad reputation that was previously popular from Kampung Kubur to Kampung Sejahtera.
Evaluation of Storage and Disposal of Household Waste in the City of Kinshasa (Communes of Lemba, Mont-Ngafula, and Kisenso) and Awareness of Eco-Citizenship
Authors: Frey Sylvestre , Anzola Nestor K. , Makanua Diansambu 
Published: August 2023

This study aims to analyze and describe the characteristics of household waste in the communes of Lemba, Mont-Ngafula and Kisenso in Kinshasa, while raising awareness of ecological citizenship. The study was conducted from April 17 to May 3, 2023, with a sample of 690 households, 138 randomly selected from each community. The main methods used are survey, interview and observation in the field.
The results show that household waste management in the cities studied does not contribute to sustainable development. Most households use garbage cans, mainly buckets and bags, but waste sorting is rare, except in the Kindele area. The most common waste disposal methods are inappropriate local disposal and incineration. The most common methods of waste disposal are disposal in inappropriate locations and incineration. Average waste weights vary by neighbourhood, with values ranging from 8.67 kg to 13.99 kg per household. It has a significant correlation between the total weight of waste generated per household and household size.
The findings of the study indicate that the state of household waste management in these cities is worrying, with adverse consequences for the environment and public health. Proposals include new laws to regulate waste management, integration of waste management into urban development plans, establishment of landfills and public awareness of sanitation rules and the principles of waste reduction, reuse and recycling. Developing effective and sustainable strategies to address household waste management in these communities in Kinshasa is crucial. The involvement of local authorities, stakeholders and citizens is essential to protect the environment.
Enhancing Growth and Yield of Parching Sorghum through Organic Manure Application
Authors: Potadar Jyoti , Patil M. B. , Nooli S. S. 
Published: August 2023

Aims: The combination of various sources of organic manures along with liquid organic manures is important to maintain nutrient availability to crop and sustain higher level of soil fertility. The aim is to meet the nutrient demand of parching sorghum as per requirement at different stages through solid and liquid organic manures which can give greater productivity of crops.
Study Design: The experiment was laid in Randomized Complete Block Design with 9 treatments replicated thrice.
Place and Duration of Study: A field experiment was conducted at College of Agriculture, Vijayapur, during Rabi, 2020-21.
Methodology: The treatments were T1-Ghanajeevamrutha based on 100% RDN (recommended dose of Nitrogen) as basal dose, T2-Vermicompost based on 100% RDN as basal dose, T3- T1 + foliar application of 10% vermiwash at 20 & 45 DAS (days after sowing), T4- T1 + foliar application of 10% cow urine at 20 & 45 DAS, T5- T1 + foliar application of 25% jeevamrutha at 20 & 45 DAS, T6- T2 + foliar application of 10% vermiwash at 20 & 45 DAS, T7- T2 + foliar application of 10% cow urine at 20 & 45 DAS, T8- T2 + foliar application of 25% jeevamrutha at 20 & 45 DAS and T9- Organic RPP of parching sorghum with SMJ-1 variety of parching sorghum.
Results: The application of ghanajeevamrutha based on 100 per cent RDN as basal dose + foliar application of 25 per cent jeevamrutha at 20 and 45 days after sowing recorded significantly higher dry matter production (55.86 g plant-1), leaf area index (3.49), raw and roasted grain weight (39.49 g and 51.73 g respectively), raw and roasted grain yield (1063 kg ha-1 and 1505 kg ha-1 respectively) of parching sorghum (hurda) at harvest as compared to other treatments. In addition to improvement in the major plant nutrient uptake were recorded with incorporation of ghanajeevamrutha or vermicompost along with the liquid organic manure.
Conclusion: The application of ghanajeevamrutha based on 100 per cent RDN as basal dose coupled with foliar application of 25 per cent jeevamrutha at 20 and 45 days after sowing recorded significantly higher growth, yield, quality and plant nutrient uptake by parching sorghum.
Prediction Model for Early-Stage Pancreatic Cancer Using Routinely Measured Blood Biomarkers
Authors: Boyd Lenka N. C. , Ali Mahsoem , Comandatore Annalisa , Garajova Ingrid , Kam Laura , Puik Jisce R. , Fraga Rodrigues Stephanie M. , Meijer Laura L. , Le Large Tessa Y. S. , Besselink Marc G. , Morelli Luca , Frampton Adam , van Laarhoven Hanneke W. M. , Giovannetti Elisa , Kazemier Geert 
Published: August 2023

ImportanceAccurate risk prediction models using routinely measured biomarkers—eg, carbohydrate antigen 19-9 (CA19-9) and bilirubin serum levels—for pancreatic cancer could facilitate early detection of pancreatic cancer and prevent potentially unnecessary diagnostic tests for patients at low risk. An externally validated model using CA19-9 and bilirubin serum levels in a larger cohort of patients with pancreatic cancer or benign periampullary diseases is needed.ObjectiveTo assess the discrimination, calibration, and clinical utility of a prediction model using readily available blood biomarkers (carbohydrate antigen 19-9 [CA19-9] and bilirubin) to distinguish early-stage pancreatic cancer from benign periampullary diseases.Design, Setting, and ParticipantsThis diagnostic study used data from 4 academic hospitals in Italy, the Netherlands, and the UK on adult patients with pancreatic cancer or benign periampullary disease treated from 2014 to 2022. Analyses were conducted from September 2022 to February 2023.ExposuresSerum levels of CA19-9 and bilirubin from samples collected at diagnosis and before start of any medical intervention.Main Outcomes and MeasuresDiscrimination (measured by the area under the curve [AUC]), calibration, and clinical utility of the prediction model and the biomarkers, separately.ResultsThe study sample comprised 249 patients in the development cohort (mean [SD] age at diagnosis, 67 [11] years; 112 [45%] female individuals), and 296 patients in the validation cohort (mean [SD] age at diagnosis, 68 [12] years; 157 [53%] female individuals). At external validation, the prediction model showed an AUC of 0.89 (95% CI, 0.84-0.93) for early-stage pancreatic cancer vs benign periampullary diseases, and outperformed CA19-9 (difference in AUC [ΔAUC], 0.10; 95% CI, 0.06-0.14; P &amp;lt; .001) and bilirubin (∆AUC, 0.07; 95% CI, 0.02-0.12; P = .004). In the subset of patients without elevated tumor marker levels (CA19-9 &amp;lt;37 U/mL), the model showed an AUC of 0.84 (95% CI, 0.77-0.92). At a risk threshold of 30%, decision curve analysis indicated that performing biopsies based on the prediction model was equivalent to reducing the biopsy procedure rate by 6% (95% CI, 1%-11%), without missing early-stage pancreatic cancer in patients.Conclusions and RelevanceIn this diagnostic study of patients with pancreatic cancer or benign periampullary diseases, an easily applicable risk score showed high accuracy for distinguishing early-stage pancreatic cancer from benign periampullary diseases. This model could be used to assess the added diagnostic and clinical value of novel biomarkers and prevent potentially unnecessary invasive diagnostic procedures for patients at low risk.
Chimaeridae
: Sensory Structures
Authors: Ferrando Sara , Gallus Lorenzo , Aicardi Stefano , Amaroli Andrea , Vacchi Marino 
Published: August 2023

The peripheral olfactory organ of Chimaeridae is a lamellar organ. The sensory epithelium is a pseudostratified epithelium with ciliated supporting cells. The olfactory neurons have a rod‐shaped appendage or microvilli at their dendritic pole, and they can be highlighted by using, as a marker, an antibody against the heterotrimeric G protein o‐type. The sensory epithelium lacks proper mucous cells and all the supporting cells show mucous granule in their apical part.
The canals of the lateral line are open grooves, with supporting C‐shaped rings of cartilgae, and they can be narrow or wide. The wide grooves present expanded dilations and they are autapomorphic character of the family. In the grooves, there are elongated neuromasts.
Chondroitin Sulfate Improves Mechanical Properties of Gelatin Hydrogel for Cartilage Regeneration in Rats
Authors: Lei Tao , Tong Zhicheng , Zhai Xinrang , Zhao Yushuang , Zhu Huangrong , Wang Lu , Wen Zhengfa , Song Binghua 
Published: August 2023

AbstractCartilage injury is a common disease in daily life. Especially in aging populations, the incidence of osteoarthritis is increasing. However, due to the poor regeneration ability of cartilage, most cartilage injuries cannot be effectively repaired. Even cartilage tissue engineering still faces many problems such as complex composition and poor integration of scaffolds and host tissues. In this study, chondroitin sulfate, one of the main components of extracellular matrix (ECM), is chosen as the main natural component of the material, which can protect cartilage in a variety of ways. Moreover, the results show that the addition of chondroitin sulfate improves the mechanical properties of gelatin methacrylate (GelMA) hydrogel, making it able to effectively bear mechanical loads in vivo. Further, chondroitin sulfate is modified to obtain the oxidized chondroitin sulfate (OCS) containing aldehyde groups via sodium periodate. This special group improves the interface integration and adhesion ability of the hydrogel to host cartilage tissue through schiff base reactions. In summary, GelMA/OCS hydrogel is a promising candidate for cartilage regeneration with good biocompatibility, mechanical properties, tissue integration ability, and excellent cartilage repair ability.