bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemic Characterization of Hand, Foot, and Mouth Disease in Jiaxing City, China
Authors: Lv Shencong , Ji Jimei , Yan Yong , Li Lina , Song Yin , Zhu Guoying 
Published: March 2023

Abstract
Objective
Our study aimed to analyze the main pathogenic characteristics of Hand, Foot and Mouth Disease (HFMD) in Jiaxing City in 2022 and analyze enterovirus (EV)-positive samples that could not be typed by gene sequencing to provide insights for further prevention and control of HFMD.
Methods
Nucleic acid sequencing of enteroviruses was performed on 585 clinical samples from Jiaxing City in 2022, and Nucleic acid sequencing and molecular characterization of the VP1 region were performed on 30 strains of EV-positive samples.
Results
Among the 585 clinical samples, CV-A6, CV-A16, and CV-A10 samples were 51.6%, 15.6%, and 12.1%, respectively, while EV-positive but unsuccessfully typed samples were 13.3%. Sequence analysis revealed that 12 strains of CV-A4, 2 strains of CV-B3, 6 strains of CV-A6, and 2 strains of E-18 were successfully identified in the 30 strains of EV-positive samples. CV-A4 was found to be the most abundant strain.
Conclusion
The prevalence of HFMD in Jiaxing City in 2022 was dominated by CV-A6, supplemented with CV-A16, CV-A10, and other enteric strains. CV-A4 was the most abundant strain among the untyped EV-positive samples, and its prevalence needs to be closely monitored.
M2 macrophage-derived exosomes carry miR-142-3p to restore the differentiation balance of irradiated BMMSCs by targeting TGF-β1
Authors: Huang Chong , Zhao Lu , Xiao Yun , Tang Zihao , Li Jing , Guo Kai , Tian Lei , Zong Chunlin 
Published: March 2023

Abstract
Purpose Radiotherapy is essential to cancer treatment, while it inevitably injures the surrounding normal tissues, and bone tissue is one of the most common sites prone to irradiation. Bone marrow mesenchymal stem cells (BMMSCs) are sensitive to irradiation and the irradiated dysfunction of BMMSCs may be closely related to irradiation-induced bone damage. Macropahges paly important role in stem cell function regulation, bone metabolic balance and irradiation response, but the effects of macrophages on irradiated BMMSCs are still unclear. This study aimed to investigate the role of macrophages and macrophage-derived exosomes in restoring irradiated BMMSCs function.
Methods The effects of macrophage conditioned medium (CM) and macrophage-derived exosomes on osteogenic and fibrogenic differentiation capacities of irradiated BMMSCs were detected. The key microribonucleic acids (miRNAs) and targeted proteins in macrophage-derived exosomes were also determined.
Results The results showed that X-ray irradiation significantly inhibited the proliferation of BMMSCs. Additionally, it caused a differentiation imbalance of BMMSCs, with decreased osteogenic differentiation and increased fibrogenic differentiation. M2 macrophage-derived exosomes (M2D-exos) inhibited the fibrogenic differentiation and promoted the osteogenic differentiation of irradiated BMMSCs. We identified that miR-142-3p was significantly overexpressed in M2D-exos and irradiated BMMSCs treated with M2D-exos. After inhibition of miR-142-3p in M2 macrophage, the effects of M2D-exos on irradiated BMMSCs differentiation were eliminated. Furthermore, transforming growth factor beta 1 (TGF-β1), as a direct target of miR-142-3p, was significantly decreased in irradiated BMMSCs treated by M2D-exos.
Conclusion This study indicated that M2D-exos could carry miR-142-3p to restore the differentiation balance of irradiated BMMSCs by targeting TGF-β1. These findings pave the way for a new, promising, and cell-free therapeutic method to treat radiation-induced bone damage.
Simultaneous presentation and resection of esophageal cancer and metastasis to the pancreas: a case report with literature review
Authors: Denda Yuki , Yoichi Matsuo , Nonoyama Keisuke , Murase Hiromichi , Kato Tomokatsu , Hayashi Yuichi , Imafuji Hiroyuki , Saito Kenta , Tanaka Tatsuya , Morimoto Mamoru , Kato Hiroyuki , Yoshida Michihiro , Naitoh Itaru , Hayashi Kazuki , Ogawa Ryo , Takahashi Hiroki , Takiguchi Shuji 
Published: March 2023

Abstract
Background
The frequency of metastasis to the pancreas is very low, and the frequency of metastasis from squamous cell carcinoma of the esophagus is even lower. Although curative resection of these metastatic lesions has been reported for some patients, the survival benefit of these procedures has not yet been clearly determined.
Case presentation
The patient was a 54-year-old man who was found to have lower thoracic esophageal cancer. Computed tomography showed a 2-cm tumor in the tail of his pancreas. Since no other obvious distal metastases were observed, the patient underwent simultaneous surgical procedures that excised both the esophageal squamous cell carcinoma and the pancreatic metastasis. Histopathologic examination confirmed squamous cell carcinoma in both specimens. The patient has been free of disease for 9 months since the resection. A literature review of all relevant cases to date found that the site of the primaries of all cases of esophageal cancers with metastasis to the pancreas was the lower thoracic esophagus.
Conclusion
Complete simultaneous resections of esophageal squamous cell carcinoma and a solitary metastasis to the pancreas is beneficial and may produce good outcomes. However, because of the low number of such reports, further studies are needed to confirm the benefits of surgery.
Clinicopathological Features and Outcomes of PLA2R-Related Membranous Nephropathy with Renal Glycosuria
Authors: Zhang Piao , Xu Feng , Hu Ziyun , Liang Dandan , Liang Shaoshan , Zhu Xiaodong , Yang Fan , Zeng Caihong 
Published: March 2023

Abstract
Background
Membranous nephropathy (MN) is an immune complex mediated disease. The loss of massive proteinuria can lead to Fanconi syndrome, clinically, manifesting as renal glycosuria. The prevalence and prognosis of M type phospholipase A2 receptor (PLA2R)-related MN with renal glycosuria remain unknown.
Methods
Patients diagnosed as PLA2R-related MN with renal glycosuria were reviewed, and the control group comprised of patients with MN without renal glycosuria who were randomly selected in a ratio of 1:3.
Results
From January 2015 to January 2020, a total of 50 patients identified as PLA2R-related MN with renal glycosuria were included, with a prevalence of 2.3%. Compared with patients without renal glycosuria, those with renal glycosuria exhibited greater proteinuria, lower estimated glomerular filtration rate (eGFR), as well as higher use of diuretics, anticoagulants, antibiotics, traditional Chinese medicine and tacrolimus within 3 months prior to renal biopsy (all p < 0.05). Histologically, patients with renal glycosuria exhibited more severe degrees in pathological stages, acute/chronic tubulointerstitial lesions and tubulointerstitial inflammation (all p < 0.05). Of the 10 cases treated with rituximab (RTX), 6 (60%) maintained proteinuria remission, and 5/6 (83.3%) achieved urine glucose remission. Multivariate Cox regression analysis showed that renal glycosuria and age > 50 years were independent risk factors for end-stage renal disease (ESRD) or 30% reduction of eGFR in patients with PLA2R-related MN.
Conclusion
PLA2R-related MN patients with renal glycosuria presented with more severe clinicopathological manifestations and worse prognosis. Suspected nephrotoxic drugs should be used rationally, and RTX could be used as a treatment option.
Application of an Adjustable Bilateral Traction Device with PVC Pipes in Lower Limb Fractures
Authors: Yang Shuye , Sun Hongshuo , Liu Hongzhi , Li Peng , Du Gangqiang , Jiang Jianhao , Song Kaikai , Zhang Xinjun , Jia Long , Zhang Kai , Wang Zhigang 
Published: March 2023

Abstract
OBJECTIVE: To introduce a new type of simple adjustable bilateral bi-directional traction device of PVC (Polyvinylchloride) tube, and discusses the application value of this device before operation in patients with lower limb fractures.
METHODS: To introduce the manufacturing process of adjustable bilateral traction device of PVC pipes. From August 2018 to November 2019, the data of 36 patients with lower limb fractures treated with this traction device were analyzed retrospectively. The results of treatment were analyzed, including limb length of both lower limbs, fracture reduction, lower limb mobility, visual analogue scale (VAS), incidence of complications, and patient satisfaction.
RESULTS: All patients can perform MRI examination immediately after using the device, which is conducive to rapid and comprehensive diagnosis of diseases. The patient's pain is significantly reduced and were free to turn over during bed rest, with the length of the affected limb restored to equal to that of the healthy limb. 34 patients were satisfied with the reduction of fractured end, 2 patients with tibiofibular fracture showed angular displacement of fractured end, and the reduction was satisfactory after adjusting the position of bone traction needle; 7 patients had deep venous thrombosis of affected lower limb during traction; no pressure sore, vascular nerve injury, with an overall complication rate of 25% (9/36). All patients and their families were satisfied with this treatment the results.
CONCLUSION: The device can be temporarily assembled by the physician according to the specific conditions of different heights, fracture sites and uses of patients according to the needs of clinical treatment, and also has the advantages of economy, practicality, simple use and light weight. The device proved to be an effective and acceptably safe device for patients with lower limb fractures before operation temporary treatment. Especially suitable for patients with severe combined injury who need MRI examination urgently.
High incidence of catheter-associated urinary tract infections and related antibiotic resistance in two hospitals of different geographic regions of Sierra Leone: a prospective cohort study
Authors: LAKOH Sulaiman , Yi Le , RUSSELL James B.W. , ZHANG Juling , SEVALIE Stephen , ZHAO Yongkun , KANU Josep Sam , LIU Peng , CONTEH Sarah K. , WILLIAMS Christine Ellen Elleanor , BARRIE Umu , ADEKANMBI Olukemi , JIBA Darlinda F. , KAMARA Matilda N. , SESAY Daniel , DEEN Gibrilla F. , OKEIBUNOR Joseph Chukwudi , YENDEWA George A. , GUO Xuejun , FIRIMA Emmanuel 
Published: March 2023

Abstract
Objective
Catheter-associated urinary tract infections (CAUTI) are common worldwide, but due to limited resources, its actual burden in low-income countries is unknown. Currently, there are gaps in knowledge about CAUTI due to lack of surveillance activities in Sierra Leone. In this prospective cohort study, we aimed to determine the incidence of CAUTI and associated antibiotic resistance in two tertiary hospitals in different regions of Sierra Leone.
Results
The mean age of the 459 recruited patients was 48.8 years. The majority were females (236, 51.3%). Amongst the 196 (42.6%) catheterized patients, 29 (14.8%) developed CAUTI. Bacterial growth was reported in 32 (84%) patients. Escherichia coli (14, 23.7%), Klebsiella pneumoniae (10, 17.0%), and Klebsiella oxytoca (8, 13.6%) were the most common isolates. Most isolates were ESBL-producing Enterobacteriaceae (33, 56%) and WHO Priority 1 (Critical) pathogens (38, 71%). Resistance of K. pneumoniae, K. oxytoca, E. coli, and Proteus mirabilis was higher with the third-generation cephalosporins and penicillins but lower with carbapenems, piperacillin-tazobactam and amikacin. To reduce the high incidence of CAUTI and multi-drug resistance organisms, urgent action is needed to strengthen the microbiology diagnostic services, integrate multi-drug resistance organism surveillance into the routine clinical services and establish functional antibiotic stewardship programs in these hospitals.
Technology-enabled Learning in Digital Education: Research Areas and Prediction Trends of Learning Technologies for Authentic Assessments Practices
Authors: Lim Tristan , Gottipati Swapna , Cheong Michelle 
Published: March 2023

Abstract
This study aims to provide practical insights on the research areas and prediction trends of learning technologies that support authentic assessment practices in the digital education setting. Technology-enabled learning can be utilized to enhance assessment design in meaningful ways that resembles the professional work environment. Innovative technology-enabled assessment practices necessitate an understanding of the technologies at-hand, hence a disciplined inquiry is called upon into the multitude of technologies that define the landscape. The study looked to investigate the technology groups and research efforts within the field of learning technologies that were shaping digitally mediated authentic assessment practices. Using Horizon Report (HR), an annual education-based technology forecasting publication, as a reference, the study looked to evaluate if HR is a good proxy to predict the evolution of learning technologies in the said scape. Research distinguished four key learning technology groups, namely ubiquitous learning technology, adaptive learning technology, immersive learning technology, and learning object. Network analyses performed on the four technology groups identified key generalizable research themes that can be utilized for research focus. The study also found that HR is a useful tool to predict technology trends, as corroborated from bibliometric analyses, and detailed the composition and evolution of these trends. This study can help educators and researchers identify and decide promising potential areas for future research and/or investment focus.
Chances and barriers of building information modelling in wastewater management
Authors: Kretschmer Florian , Franziskowski S. , Huber F. , Ertl T. 
Published: March 2023

Abstract
The advancing digitalisation is one of the great challenges of our times. Related activities also concern the wastewater sector. In the field of building construction, one emerging approach is building information modelling (BIM). The presented work investigates to which extent BIM practices have already found their way to wastewater management, and what kind of benefits and constraints are incorporated. Information is collected by means of a literature review and international expert surveys. Results indicate that several BIM-related key elements are already well established in the sector, but not necessarily in the intended manner. Consequently, the digital transition in the wastewater sector is not about replacing existing procedures and techniques but to rethink and optimise them. This primarily concerns data and information management in combination with the application of digital tools. Furthermore, wastewater management requires more integrated approaches, involving interdisciplinary/collaborative concepts and life cycle perspectives. Appropriate change management is necessary to give support and guidance to employees during the transition process. Furthermore, also from the political side, a clear definition and communication of the pursued digital vision is important. This article aims at stimulating discussion and research to optimise wastewater management from the digital perspective.
Esensialisme dan Perspektifnya terhadap Program Merdeka Belajar Kampus Merdeka
Authors: Richardo Rino , Cahdriyana Rima Aksen 
Published: March 2023

<p class="15">Tujuan penelitian untuk mendeksripsikan konsep filsafat esensialisme, pandangan esensialisme terhadap (belajar, pendidik dan sekolah), program Merdeka Belajar Kampus Merdeka (MBKM), serta posisi esensialisme dalam program MBKM. Penelitian ini merupakan studi pustaka (<em>library research</em>). Sumber data merupakan buku dan artikel ilmiah elektronik. Teknik pengumpulan data yaitu menelusuri data secara online melalui database google cendikia. Sedangkan analisis data dalam penelitian ini menggunakan metode analisis isi. Hasil kajian dalam studi ini menunjukkan bahwa esensialisme sebagai filsafat pendidikan berperan dalam memberikan dasar pijakan dalam mengembangkan kebijakan redesain kurikulum berbasis MBKM, sehingga kegiatan MBKM dapat memfasilitasi tercapainya kompetensi utama sekaligus kompetensi tambahan bagi mahasiswa.</p>
Sugarcane streak mosaic virus (ScSMV) Resistance Evaluation of Sugarcane Varieties
Authors: Ernawati Feny , Djauhari Syamsuddin , Martosudiro Mintarto , Putra Lilik Koesmihartono , Kristini Ari 
Published: March 2023

Sugarcane streak mosaic virus (ScSMV) is the most important viral disease of sugarcane in Indonesia with distribution in almost all commercial sugarcane plantations. The disease causes significant yield losses of both cane tonnage and sugar yield. The use of resistant varieties is the best approach for controlling viral diseases. This study aims to investigate resistance response of several introduced varieties against ScSMV in a glasshouse condition and the impact of the viral infection on chlorophyll and proline content in sugarcane leaves. Sugarcane plants were inoculated using ScSMV inoculum one month after planting using an abrasive pad rubbing method. Disease incidence and severity was observed at week 4-12 after inoculation and variety resistance levels were classified based on disease incidence. Confirmation of the virus was done by RT-PCR. Spectrophotometer was used to measure chlorophyll content at dual wavelengths of 645 and 663 nm, and proline content at wavelengths of 520 nm. The results showed that most of the tested varieties were susceptible to ScSMV. There are six highly resistant varieties, namely SRA 1, SRA 2, N 10-4, N 10-7, N10-9, and N 10-13, but these varieties still require to be tested on a field scale. ScSMV infection generally decrease chlorophyll and proline content. However, the physiological effect of ScSMV infection on chlorophyll and proline content needs further investigation.
Impact of Early Life Factors on Metabolic Phenotypes of Obesity in Preschool Children
Authors: Liao Zijun , Xie Xianghui , Wang Jing , Chen Yiren , Zhang Ting , Liu Gongshu , Chen Fangfang 
Published: March 2023

OBJECTIVETo investigate the impact of factors in the first 1,000 days of life on metabolic phenotypes of obesity in preschool children in a cohort study.RESEARCH DESIGN AND METHODSWe recruited 3-year-old children for the study. Early life factors included maternal age at delivery, maternal education, prepregnancy BMI, gestational weight gain, gravidity, history of gestational diabetes mellitus, delivery mode, gestational age, family history of metabolic disorders, paternal education, annual family income, child sex, birth weight, and breastfeeding duration. According to BMI and metabolic status, children were classified as metabolically healthy (no metabolic risk factors) with normal weight (MHNW), metabolically unhealthy (one or more metabolic risk factors) with normal weight (MUNW), metabolically healthy with overweight or obesity (MHO), and metabolically unhealthy with overweight or obesity (MUO).RESULTSWe recruited 3,822 children for the study, with 3,015 analyzed. Accelerated BMI z score growth rate between 6 and 24 months was associated with MHO (β = 0.022; 95% CI 0.009, 0.036) and MUO (β = 0.037; 95% CI 0.018, 0.056). Maternal overweight (odds ratio [OR] 3.16; 95% CI 1.55, 6.42) and obesity (OR 8.14; 95% CI 3.73, 17.76) before pregnancy and macrosomia (OR 2.47; 95% CI 1.32, 4.59) were associated with MHO, and maternal obesity before pregnancy (OR 6.35; 95% CI 2.17, 18.52) increased the risk of MUO.CONCLUSIONSEarly life factors, such as maternal obesity and accelerated BMI growth rate between 6 and 24 months, were related not only to MHO but also to MUO. Children with these early life factors should be given interventions for weight control to prevent metabolic abnormalities.
Investigating an Alternative for Estimation from a Nonprobability Sample: Matching plus Calibration
Authors: Liu Zhan , Valliant Richard 
Published: March 2023

Abstract
Matching a nonprobability sample to a probability sample is one strategy both for selecting the nonprobability units and for weighting them. This approach has been employed in the past to select subsamples of persons from a large panel of volunteers. One method of weighting, introduced here, is to assign a unit in the nonprobability sample the weight from its matched case in the probability sample. The properties of resulting estimators depend on whether the probability sample weights are inverses of selection probabilities or are calibrated. In addition, imperfect matching can cause estimates from the matched sample to be biased so that its weights need to be adjusted, especially when the size of the volunteer panel is small. Calibration weighting combined with matching is one approach to correct bias and reduce variances. We explore the theoretical properties of the matched and matched, calibrated estimators with respect to a quasirandomization distribution that is assumed to describe how units in the nonprobability sample are observed, a superpopulation model for analysis variables collected in the nonprobability sample, and the randomization distribution for the probability sample. Numerical studies using simulated and real data from the 2015 US Behavioral Risk Factor Surveillance Survey are conducted to examine the performance of the alternative estimators.
The role of leukotriene B4 in cow metritis
Authors: Wang Guo-Qing , Zheng Hao-Yue , Hou Jin-Long , Wang Cui , Sun Hui-Lian , Wang Lan 
Published: March 2023

Abstract

Introduction
Metritis is a common postpartum disease in dairy cows. As a mast cell (MC) mediator, leukotriene B4 (LTB4) is the strongest phagocyte chemokine. It is important in inflammation for the recruitment of immune cells to resist infection. This study investigated the effect of LTB4 in metritis.

Material and Methods
Twenty Holstein cows 3 to 6 years old and at 6 to 10 days postpartum were selected, ten of which with postpartum metritis were the experimental group, and the other ten of which as healthy cows were the control group. The levels of LTB4, substance P (SP) and vasoactive intestinal peptide (VIP) were measured by ELISA, the expression of LTB4 receptor 2 (BLT2), matrix metalloproteinase (MMP)-2 and MMP-9 mRNA was measured by qPCR, and collagens I and IV were detected by immunohistochemical staining.

Results
Concentrations of SP and LTB4 were significantly higher, but those of VIP were significantly lower in the experimental group than those in the control group. The expression of BLT2, MMP-2 and MMP-9 mRNA was significantly higher in the experimental group than that in the control group. The expression of collagen Ⅰ and collagen Ⅳ was significantly lower in the experimental group than that in the control group.

Conclusion
In metritis, SP promotes the activation of MC and the synthesis and release of LTB4. Leukotriene B4 chemotactic immune cells promote the high expression of collagenase, which accelerated the hydrolysis of collagen, while the inhibitory effect of VIP on MC was weakened. This may further aggravate the damage to uterine tissue.