bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of the ELISA Method for Neutralizing Anti-trastuzumab Antibodies Detection in Human Blood Serum
Authors: Kolganova M. A. , Sagimbaeva O. S. , Borisova Ju. S. , Beketov E. E. , Shokhin I. E. 
Published: May 2023

Introduction. Trastuzumab is the first known anti-HER2 agent, which revolutionized the treatment of one of the most common cancer types – breast cancer. Despite trastuzumab being approved long time ago, further improvement of related analytical methods remains relevant primarily due to the emergence of new biosimilars. For instance, immunogenicity – adverse reaction which is usually associated with biological drugs, can still be relevant for trastuzumab. Anti-drug antibodies, including neutralizing antibodies, caused by trastuzumab therapy, can affect drug effectiveness and safety profile.Aim. The aim of this study was to develop and validate the analytical method for neutralizing anti-trastuzumab antibodies determination in human blood serum.Materials and methods. The neutralizing anti-trastuzumab antibody determination was carried out by the competitive ELISA method, using spectrophotometric detection in the visible range of the spectrum.Results and discussion. The developed method was validated for cut-point, selectivity, sensitivity, specificity, precision and stability (short-term and long-term). To decrease the background noise from non-specific binding of sera components, the minimum required dilution value was determined at 0.5 % serum. The calculated value for cut-point was 14.62 %. The sensitivity of the developed method was estimated at 1985.2 ng/mL of neutralizing anti-trastuzumab antibodies.Conclusion. The obtained results allowed us to apply the developed ELISA method for the neutralizing anti-trastuzumab antibodies determination in human blood serum during trastuzumab immunogenicity assessment in bioequivalence clinical trials.
Bioactive Xanthone C-glycoside Derivatives – QSAR Approach
Authors: Aksenova V. N. , Morozova M. A. , Syroeshkin A. V. 
Published: May 2023

Introduction. Xanthone glycosides have unique structures and properties. Many efforts focus on the search for C-glycoside derivatives of mangiferin with higher bioavailability. The application of the QSAR approach allows for the optimization of the search for novel xanthone derivatives with the desired characteristics.Aim. Using available descriptors of chemical structure, physical-chemical properties, and biological activity, analyze a sample set of known homologs and analogs of mangiferin to QSAR prognosis bioactivity of new xanthone C-glycosides.Materials and methods. 26 molecules of natural homologs and modified derivatives of mangiferin formed the analyzed sample set. Topological graphs of compounds were constructed using ChemicPen software. ChemicDescript software was used for the calculation of molecular descriptors, including the Balaban index. Physicochemical characteristics of molecules as well as Lipinski's rule criteria were calculated in Molinspiration. The spectrum of the most probable (Pa > 0.7) biological activity of the described compounds were predicted using Pass Online. The software Origin (OriginLab, USA) was used for the graphical representation of the results.Results and discussion. Mangiferin and its natural homologs are the most hydrophilic compounds. The hydrolysis of the C-glycosidic bond, alkylation, acylation, and the introduction of an amino substituent radical into the mangiferin structure led to the increase of its lipophilic properties. The spectrum of the most probable biological activities of the described molecules: antitumor, antioxidant, and cardioprotective effects. The results of ADMET modeling based on the substance-drug similarity criteria showed that only 4 compounds correspond to the rule of five. We proposed the validation model to predict bioactivity from lipophilicity and molecule structure described with Balaban index. The error of prediction obtained in a result of cross-validation turned out to be about less than 3 %.Conclusion. A correlation between the structure and properties of the molecules discussed has been demonstrated. The obtained results can be used for further prediction of the properties of natural and synthetic xanthone C-glycosides and directed synthesis of new active compounds.
Application of Selenium Nanoparticles in Oncology (Review)
Authors: Dukhnovsky E. A. 
Published: May 2023

Introduction. Due to the rapid development of nanotechnology, selenium nanoparticles (NPs) have recently attracted much attention due to their unique physical and chemical properties for biomedical applications, in particular for the treatment of oncological diseases. The review considers the selenium nanoparticles, which are widely studied in the field of oncology.Text. This review is devoted to the analysis of scientific literature on the anticancer activity of selenium nanoparticles against human cancer cell lines, as well as the application of these nanoparticles as a drug delivery system. Besides, the antitumor mechanisms of selenium nanoparticles against malignant neoplasms are discussed.Conclusion. According to the results of literary data analysis, it was found that selenium nanoparticles exhibit a good antitumor effect against various human cancer cell lines. It is shown that the antitumor activity of selenium nanoparticles is mainly related to activation of the extrinsic and intrinsic signaling pathways of apoptosis leading to cancer cell death. Also, selenium nanoparticles are promising systems for delivery of various anticancer drugs, providing high efficiency, bioavailability of drugs in tumor cells and minimizing toxicity to healthy cells.
Atrophy of the optic chiasm is associated with microvascular diabetic complications in type 1 diabetes
Authors: Tarkkonen Aleksi , Claesson Tor-Björn , Eriksson Marika I. , Forsblom Carol , Thorn Lena M. , Summanen Paula , Groop Per-Henrik , Putaala Jukka , Gordin Daniel , Martola Juha 
Published: May 2023

IntroductionDiabetic neuropathy and diabetic eye disease are well known complications of type 1 diabetes. We hypothesized that chronic hyperglycemia also damages the optic tract, which can be measured using routine magnetic resonance imaging. Our aim was to compare morphological differences in the optic tract between individuals with type 1 diabetes and healthy control subjects. Associations between optic tract atrophy and metabolic measures, cerebrovascular and microvascular diabetic complications were further studied among individuals with type 1 diabetes.MethodsWe included 188 subjects with type 1 diabetes and 30 healthy controls, all recruited as part of the Finnish Diabetic Nephropathy Study. All participants underwent a clinical examination, biochemical work-up, and brain magnetic resonance imaging (MRI). Two different raters manually measured the optic tract.ResultsThe coronal area of the optic chiasm was smaller among those with type 1 diabetes compared to non-diabetic controls (median area 24.7 [21.0-28.5] vs 30.0 [26.7-33.3] mm2, p&lt;0.001). In participants with type 1 diabetes, a smaller chiasmatic area was associated with duration of diabetes, glycated hemoglobin, and body mass index. Diabetic eye disease, kidney disease, neuropathy and the presence of cerebral microbleeds (CMBs) in brain MRI were associated with smaller chiasmatic size (p&lt;0.05 for all).ConclusionIndividuals with type 1 diabetes had smaller optic chiasms than healthy controls, suggesting that diabetic neurodegenerative changes extend to the optic nerve tract. This hypothesis was further supported by the association of smaller chiasm with chronic hyperglycemia, duration of diabetes, diabetic microvascular complications, as well as and CMBs in individuals with type 1 diabetes.
The Association Between Etiologies and Mortality in Acute Respiratory Distress Syndrome: A Multicenter Observational Cohort Study
Authors: Wang Yan , Zhang Linlin , Xi Xiuming , Zhou Jian-Xin ,   The China Critical Care Sepsis Trial (CCCST) Workgroup
Published: May 2023

Background: Lung-protective ventilation (LPV) strategies have been beneficial in patients with acute respiratory distress syndrome (ARDS). As a vital part of LPV, positive end-expiratory pressure (PEEP) can enhance oxygenation. However, randomized clinical trials of different PEEP strategies seem to show no advantages in clinical outcomes in patients with ARDS. A potential reason is that diverse etiologies and phenotypes in patients with ARDS may account for different PEEP responses, resulting in variations in mortality. We consider hospital mortality to be associated with a more specific classification of ARDS, such as sepsis induced or not, and pulmonary or extrapulmonary one. Our study aimed to compare clinical outcomes in various patients with ARDS by etiologies using the China Critical Care Sepsis Trial (CCCST) database. This was a retrospective analysis of a prospective cohort of 2,138 patients with ARDS in the CCCST database. According to ARDS induced by sepsis or not and medical history, patients were stratified into different four groups. Differences among groups were assessed in hospital mortality, ventilation-free days, and other clinical features.Results: A total of 2,138 patients with ARDS were identified in the database, including 647 patients with sepsis-induced pulmonary ARDS (30.3%), 396 patients with sepsis-induced extrapulmonary ARDS (18.5%), 536 patients with non-sepsis pulmonary ARDS (25.1%), and 559 patients with non-sepsis extrapulmonary ARDS (26.1%). The pulmonary ARDS group had higher mortality compared with the extrapulmonary group (45.9 vs. 23.0%, p &lt; 0.01), longer intensive care unit (ICU) and hospital stays (9 vs. 6 days, p &lt; 0.01, 20 vs. 18 days, p = 0.01, respectively), and fewer ventilation-free days (5 vs. 9 days) in the presence of sepsis. However, the mortality in ARDS without sepsis was inverted compared with extrapulmonary ARDS (pulmonary 23.5% vs. extrapulmonary 29.2%, p = 0.04). After adjusting for the Acute Physiology and Chronic Health Evaluation II and sequential organ failure assessment scores and other clinical features, the sepsis-induced pulmonary condition was still a risk factor for death in patients with ARDS (hazard ratio 0.66, 95% CI, 0.54–0.82, p &lt; 0.01) compared with sepsis-induced extrapulmonary ARDS and other subphenotypes.Conclusions: In the presence of sepsis, hospital mortality in pulmonary ARDS is higher compared with extrapulmonary ARDS; however, mortality is inverted in ARDS without sepsis. Sepsis-induced pulmonary ARDS should attract more attention from ICU physicians and be cautiously treated.Trial registration: ChiCTR-ECH-13003934. Registered August 3, 2013, http://www.chictr.org.cn.
Plasma Exchange-Based Non-bioartificial Liver Support System Improves the Short-Term Outcomes of Patients With Hepatitis B Virus-Associated Acute-on-Chronic Liver Failure: A Multicenter Prospective Cohort Study
Authors: Chen Yuan-yuan , Li Hai , Xu Bao-yan , Zheng Xin , Li Bei-ling , Wang Xian-bo , Huang Yan , Gao Yan-hang , Qian Zhi-ping , Liu Feng , Lu Xiao-bo , Shang Jia , Li Hai , Wang Shao-yang , Zhang Yin-hua , Meng Zhong-ji ,   Chinese Chronic Liver Failure (CLIF) Consortium
Published: May 2023

Background and aims: Hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF) is a complicated syndrome with extremely high short-term mortality. Whether plasma exchange (PE) improves HBV-ACLF outcomes remains controversial. Here, PE-based non-bioartificial liver support system (NB-ALSS) effects on short-term HBV-ACLF patient outcomes were investigated.Materials and methods: HBV-ACLF patients from Chinese Acute-on-chronic Liver Failure (CATCH-LIFE) cohort receiving standard medical therapy (SMT) alone or PE-based NB-ALSS in addition to SMT were allocated to SMT and SMT+PE groups, respectively; propensity score matching (PSM) was used to eliminate confounding bias. Short-term (28/90-day and 1-year) survival rates were calculated (Kaplan-Meier).Results: In total, 524 patients with HBV-ACLF were enrolled in this study; 358 received SMT alone (SMT group), and the remaining 166 received PE-based NB-ALSS in addition to SMT (SMT+PE group). PSM generated 166 pairs of cases. In the SMT+PE group, 28-day, 90-day, and 1-year survival rates were 11.90, 8.00, and 10.90%, respectively, higher than those in the SMT group. Subgroup analysis revealed that PE-based NB-ALSS had the best efficacy in patients with ACLF grade 2 or MELD scores of 30–40 (MELD grade 3). In MELD grade 3 patients who received SMT+PE, 28-day, 90-day, and 1-year survival rates were improved by 18.60, 14.20, and 20.10%, respectively. According to multivariate Cox regression analysis, PE-based NB-ALSS was the only independent protective factor for HBV-ACLF patient prognosis at 28 days, 90 days, and 1 year (28 days, HR = 0.516, p = 0.001; 90 days, HR = 0.663, p = 0.010; 1 year, HR = 0.610, p = 0.051). For those who received SMT+PE therapy, PE-based NB-ALSS therapy frequency was the only independent protective factor for short-term prognosis (28-day, HR = 0.597, p = 0.001; 90-day, HR = 0.772, p = 0.018).Conclusions: This multicenter prospective study showed that the addition of PE-based NB-ALSS to SMT improves short-term (28/90 days and 1-year) outcomes in patients with HBV-ACLF, especially in MELD grade 3 patients. Optimization of PE-based NB-ALSS may improve prognosis or even save lives among HBV-ACLF patients.
Data Quality Improvement and Internal Data Audit of the Chinese Neonatal Network Data Collection System
Authors: Sun Jianhua , Cao Yun , Hei Mingyan , Sun Huiqing , Wang Laishuan , Zhou Wei , Chen Xiafang , Jiang Siyuan , Zhang Huayan , Ma Xiaolu , Wu Hui , Li Xiaoying , Shi Yuan , Gu Xinyue , Wang Yanchen , Yang Tongling , Lu Yulan , Zhou Wenhao , Chen Chao , Lee Shoo K. , Du Lizhong ,   The Chinese Neonatal Network
Published: May 2023

Background: The Chinese Neonatal Network (CHNN) is a nationwide neonatal network that aims to improve clinical neonatal care quality and short- and long-term health outcomes of infants. This study aims to assess the quality of the Chinese Neonatal Network database by conducting an internal audit of data extraction.Methods: A data audit was performed by independently replicating the data collection and entry process in all 58 tertiary neonatal intensive care units (NICU) participating in the CHNN. Eighty-eight data elements selected for re-abstraction were classified into three categories (critical, important, less important), and agreement rates for original and re-abstracted data were predefined. Three to five records were randomly selected at each site for re-abstraction, including one short- (0–7 days), two medium- (8–28 days), and two long-stay (more than 28 days) cases. Agreement rates for each data item were calculated for individual NICUs and across the network, respectively.Results: A total of 283 cases and 24,904 data fields were re-abstracted. The agreement rates for original and re-abstracted data elements were 96.1% overall, and 97.2, 94.3, and 96.6% for critical, important, and less important data elements, respectively. Individual site variation for discrepancies ranged between 0.0 and 18.4% for all collected data elements.Conclusion: The completeness, precision, and quality of data in the CHNN database are high, providing assurance for multipurpose use, including health service evaluation, quality improvement, clinical trials, and other research.
Optimal planting pattern of cotton is regulated by irrigation amount under mulch drip irrigation
Authors: Zuo Wenqing , Wu Baojian , Wang Yuxuan , Xu Shouzhen , Tian Jingshan , Jiu Xingli , Dong Hengyi , Zhang Wangfeng 
Published: May 2023

ObjectiveIt is of great importance to explore agronomic management measures for water conservation and cotton yield in arid areas.MethodsA four–year field experiment was conducted to evaluate cotton yield and soil water consumption under four row spacing configurations (high/low density with 66+10 cm wide, narrow row spacing, RS66+10H and RS66+10L; high/low density with 76 cm equal row spacing, RS76H and RS76L) and two irrigation amounts (CI:conventional drip irrigation; LI:limited drip irrigation) during the growing seasons in Shihezi, Xinjiang.ResultsA quadratic relationship was observed between the maximum LAI (LAImax) and seed yield. Canopy apparent transpiration rate(CAT), daily water consumption intensity (DWCI) and crop evapotranspiration (ETC) were positively and linearly correlated with LAI. The seed yields, lint yields, and ETC under CI were 6.6–18.3%,7.1–20.8% and 22.9–32.6%higher than those observed under LI, respectively. The RS66+10H under CI had the highest seed and lint yields. RS76L had an optimum LAImax range, which ensured a higher canopy apparent photosynthesis and daily dry matter accumulation and reached the same yield level as RS66+10H; however, soil water consumption in RS76L was reduced ETC by 51–60 mm at a depth of 20–60 cm at a radius of 19–38 cm from the cotton row,and water use efficiency increased by 5.6–8.3%compared to RS66+10H under CI.ConclusionA 5.0&lt;LAImax&lt;5.5 is optimum for cotton production in northern Xinjiang, and RS76L under CI is recommended for high yield and can further reduce water consumption. Under LI, the seed and lint yield of RS66+10H were 3.7–6.0% and 4.6–6.9% higher than those of RS76L, respectively. In addition, high-density planting can exploit the potential of soil water to increase cotton yields under water shortage conditions.
Research on the influence and mechanism of human–vehicle moral matching on trust in autonomous vehicles
Authors: Chen Na , Zu Yao , Song Jing 
Published: May 2023

IntroductionAutonomous vehicles can have social attributes and make ethical decisions during driving. In this study, we investigated the impact of human-vehicle moral matching on trust in autonomous vehicles and its mechanism.MethodsA 2*2 experiment involving 200 participants was conducted.ResultsThe results of the data analysis show that utilitarian moral individuals have greater trust than deontological moral individuals. Perceived value and perceived risk play a double-edged role in people’s trust in autonomous vehicles. People’s moral type has a positive impact on trust through perceived value and a negative impact through perceived risk. Vehicle moral type moderates the impact of human moral type on trust through perceived value and perceived risk.DiscussionThe conclusion shows that heterogeneous moral matching (people are utilitarian, vehicles are deontology) has a more positive effect on trust than homogenous moral matching (both people and vehicles are deontology or utilitarian), which is consistent with the assumption of selfish preferences of individuals. The results of this study provide theoretical expansion for the fields related to human-vehicle interaction and AI social attributes and provide exploratory suggestions for the functional design of autonomous vehicles.
Prognostic value of albumin-bilirubin score in pancreatic cancer patients after pancreatoduodenectomy with liver metastasis following radiofrequency ablation
Authors: Zhang Lei , Zhang Xuefei , Wu Bolin , Han Xue , Guo Cunli , Li Bo , Jing Hui , Cheng Wen 
Published: May 2023

Objective: The current study aimed to investigate the prognostic value of albumin-bilirubin (ALBI) score in predicting clinical outcomes of pancreatic cancer patients after pancreatoduodenectomy with liver metastasis following radiofrequency ablation.Methods: This retrospective study included 90 pancreatic cancer patients after pancreatoduodenectomy with liver metastasis from January 2012 to December 2018. In this study, the Chi-square or Fisher’s exact tests, the receiver operating characteristic (ROC) curve, Kaplan-Meier method and Log-rank test, univariate and multivariate Cox proportional hazard regression analyses, nomogram, calibration curves and decision curve analysis were used for all statistical analysis.Results: We analyzed the optimal cut-off value of ALBI by ROC curve, and the optimal cut-off value was −2.60. According to ALBI score, these patients were divided into two groups: low ALBI group (n = 33) and high ALBI group (n = 57). Patients with low ALBI score was significantly related to longer progression free survival (PFS) (p = 0.0002, HR: 3.039, 95% CI: 1.772–5.210) and overall survival (OS) (p = 0.0005, HR: 2.697, 95% CI: 1.539–4.720). The 1-, 3-, and 5-year PFS and OS rates in low ALBI group were higher than those in high ALBI group. ALBI was a potential independent prognostic factor for pancreatic cancer patients after pancreatoduodenectomy with liver metastasis following radiofrequency ablation. Moreover, the nomogram was used to predict the 1-, 3-, and 5-year survival probabilities of PFS and OS. The calibration curve shown that the prediction line matched the reference line well for postoperative 3-year PFS and OS. The DCA shown that nomogram model was better than the only ALBI, and indicated the ability for clinical decision-making, especially in 1-year PFS, and 3-, 5-year OS.Conclusion: ALBI is a potential independent factor for PFS and OS, and can predict the prognosis of pancreatic cancer patients after pancreatoduodenectomy with liver metastasis following radiofrequency ablation.
Obesity is Associated With Delayed Graft Function in Kidney Transplant Recipients: A Paired Kidney Analysis
Authors: Shi Bree , Ying Tracey , Xu Josephine , Wyburn Kate , Laurence Jerome , Chadban Steven J 
Published: May 2023

Obesity is increasingly prevalent among candidates for kidney transplantation. Existing studies have shown conflicting post-transplant outcomes for obese patients which may relate to confounding bias from donor-related characteristics that were unaccounted for. We used ANZDATA Registry data to compare graft and patient survival between obese (BMI &gt;27.5 kg/m2 Asians; &gt;30 kg/m2 non-Asians) and non-obese kidney transplant recipients, while controlling for donor characteristics by comparing recipients of paired kidneys. We selected transplant pairs (2000–2020) where a deceased donor supplied one kidney to an obese candidate and the other to a non-obese candidate. We compared the incidence of delayed graft function (DGF), graft failure and death by multivariable models. We identified 1,522 pairs. Obesity was associated with an increased risk of DGF (aRR = 1.26, 95% CI 1.11–1.44, p &lt; 0.001). Obese recipients were more likely to experience death-censored graft failure (aHR = 1.25, 95% CI 1.05–1.49, p = 0.012), and more likely to die with function (aHR = 1.32, 95% CI 1.15–1.56, p = 0.001), versus non-obese recipients. Long-term patient survival was significantly worse in obese patients with 10- and 15-year survival of 71% and 56% compared to 77% and 63% in non-obese patients. Addressing obesity is an unmet clinical need in kidney transplantation.