bluuebunny/crossref_metadata_2025_split huggingface dataset:
DDX24 Is Essential for Cell Cycle Regulation in Vascular Smooth Muscle Cells During Vascular Development via Binding to FANCA mRNA
Authors: Gong Yujiao , Liang Yan , Liu Jie , Wei Jiaxing , Zhang Shushan , Chen Fangbin , Zhang Qianqian , Wang Lijie , Lan Huimin , Wu Lily , Ge Wei , Li Shuai , Wang Li , Shan Hong , He Huanhuan 
Published: July 2023

BACKGROUND:
The DEAD-box family is essential for tumorigenesis and embryogenesis. Previously, we linked the malfunction of DDX (DEAD-box RNA helicase)-24 to a special type of vascular malformation. Here, we aim to investigate the function of DDX24 in vascular smooth muscle cells (VSMCs) and embryonic vascular development.

METHODS:

Cardiomyocyte (CMC) and VSMC-specific
Ddx24
knockout mice were generated by crossing
Tagln
-Cre mice with
Ddx24

flox/flox

transgenic mice. The development of blood vessels was explored by stereomicroscope photography and immunofluorescence staining. Flow cytometry and cell proliferation assays were used to verify the regulation of DDX24 on the function of VSMCs. RNA sequencing and RNA immunoprecipitation coupled with quantitative real-time polymerase chain reaction were combined to investigate DDX24 downstream regulatory molecules. RNA pull-down and RNA stability experiments were performed to explore the regulation mechanism of DDX24.

RESULTS:

CMC/VSMC-specific
Ddx24
knockout mice died before embryonic day 13.5 with defects in vessel formation and abnormal vascular remodeling in extraembryonic tissues.
Ddx24
knockdown suppressed VSMC proliferation via cell cycle arrest, likely due to increased DNA damage. DDX24 protein bound to and stabilized the mRNA of
FANCA
(FA complementation group A) that responded to DNA damage. Consistent with the function of DDX24, depletion of FANCA also impacted cell cycle and DNA repair of VSMCs. Overexpression of FANCA was able to rescue the alterations caused by DDX24 deficiency.

CONCLUSIONS:
Our study unveiled a critical role of DDX24 in VSMC-mediated vascular development, highlighting a potential therapeutic target for VSMC-related pathological conditions.
Revisiting Anticoagulation in Spine Surgery: Balancing Venous Thromboembolic Events and Epidural Hematoma
Authors: Massaro Allie M. , Frier Sven , Strot Sarah M. , Scherman Ashley , Oskouian Rod J. , Chapman Jens R. 
Published: July 2023

Study Design Retrospective Cohort Study. Objective Venous thromboembolic events (VTE) and post-operative epidural hematoma (EDH) are significant complications after spine surgery. Guidelines for chemoprophylaxis are controversial and variability amongst surgeons remains. The objective of this study was to establish the incidence of clinical VTE and EDH at our institution and evaluate the association of chemoprophylaxis with clinical VTE and EDH. Methods We conducted a retrospective cohort study of patients undergoing spine surgery at a high-volume tertiary care center in Seattle, WA between January 2016 and December 2019. The Premier Health Care Database and Agency for Healthcare Research and Quality (AHRQ) patient indicators PSI-9 (Perioperative hemorrhage and hematoma) and PSI-12(Perioperative PE or DVT) were used to identify patients experiencing VTE and/or post-operative EDH. The primary outcome was the incidence of clinical VTE and EDH in post-operative spine patients. Secondary outcomes included the association of chemoprophylaxis with clinical VTE and EDH. Results From 2016 to 2019, 4587 patients underwent spine surgery, totaling 4764 hospital stays. The incidence of clinical VTE was .21% (10/4764) and the incidence of EDH was .10% (5/4764). Most hemorrhages occurred prior to the initiation of chemoprophylaxis. One patient with EDH received chemoprophylaxis prior to hemorrhage. Conclusions The rate of post-operative clinical VTE and EDH in spine surgery is low. Despite early initiation of chemoprophylaxis after major spine surgery we did not appreciate a high rate of EDH. We attribute our low rate of clinical VTE to multimodal prophylaxis with SCDs, early mobilization and chemoprophylaxis on post-operative day 1.
Salvage surgery for advanced non-small cell lung cancer following previous immunotherapy: a retrospective study
Authors: Higuchi Mitsunori , Inomata Sho , Yamaguchi Hikaru , Saito Takuro , Suzuki Hiroyuki 
Published: July 2023

Abstract
Background
The development of systemic chemotherapy including immune checkpoint inhibitors (ICIs) has provided patients with unresectable advanced non-small cell lung cancer (NSCLC) an opportunity to undergo surgical intervention after initial treatment. However, no consensus regarding the indication for salvage surgery in these patients has been reached.

Methods
We conducted a retrospective study of patients who underwent salvage surgery for advanced NSCLC (cStage IIIA–IVB) after treatment with ICIs from January 2018 to December 2022 at Aizu Medical Center and Fukushima Medical University Hospital. We evaluated the patients’ clinical data, calculated disease-free survival (DFS) and overall survival (OS), and assessed the survival benefit using the Kaplan–Meier method.

Results
Thirteen patients underwent salvage surgery after immunotherapy. All patients achieved downstaging after initial chemotherapy. Eleven patients underwent lobectomy, and one patient underwent extirpation of intra-abdominal lymph nodes. The mean surgery time and intraoperative blood loss were 242.2 min and 415.1 g, respectively. The mean drainage period was 4.2 days (range, 2–9 days). Grade ≥ 3 postoperative complications were confirmed in three patients. The 2-year DFS rate was 71.2%, and the 2-year OS rate was 76.2%. A pathological complete response compatible with ypStage 0 was achieved in four (30.8%) patients. Patients with ypStage 0 and I achieved significantly better OS than those with ypStage ≥ II (p = 0.044), and patients without severe complications achieved significantly better DFS and OS than those with severe complications (p = 0.001 and p < 0.001, respectively).

Conclusions
Salvage surgery after chemotherapy including ICIs is a feasible and effective treatment option for patients with advanced NSCLC, especially those who acquire downstaging to pathological stage 0 or I. However, severe perioperative complications might affect patient survival. A prospective study is urgently needed to evaluate the efficacy of salvage surgery.
The Basic Core of a Parallel Machines Scheduling Game
Authors: Alon Tzvi , Anily Shoshana 
Published: July 2023

Problem definition: We consider the parallel machine scheduling (PMS) under job-splitting game defined by a set of manufacturers where each holds uniform parallel machines and each is committed to produce some jobs submitted to her by her clients while bearing the cost of the sum of completion times of her jobs on her machines. An efficient algorithm for this scheduling problem is well known. We consider the corresponding cooperative game, where the manufacturers are players that want to join forces. We show that collaboration is profitable. Yet, the stability of the cooperation depends on the cost allocation scheme; we focus on the core of the game. Methodology/results: We prove that the PMS game is totally balanced and its core is infinitely large, by developing a sophisticated methodology of linear complexity that finds a line segment in its symmetric core. We call this segment the basic core of the game. Managerial implications: This PMS game has the potential for various applications both in traditional industry and in distributed computing systems in the hi-tech industry. The formation of a partnership among entrepreneurs, companies, or manufacturers necessitates not only a plan for joining forces toward the achievement of the ultimate goals, but also an acceptable agreement regarding the cost allocation among the partners. Core allocations guarantee the stability of the partnership as no subset of players can gain by defecting from the grand coalition. Funding: This work was supported by the Henry Crown Israeli Institute for Business Research, the Coller Foundation, and the Israel Science Foundation [Grant 1489/19].
BEACH PROFILE CHANGES INDUCED BY STORMS
Authors: To Dang Van 
Published: July 2023

Along with the introduction of the SBEACH model. the storm-induced beach profile changes in the Gold Coast. Australia, were simulated using SBEACH with survey data collected in 1996. SBEA CH qualitatively modeled the profile changes: onshore erosion and offshore deposition. Quantitatively, SBEACH did not simulate accurately the shape of the measured profile change in spite of the correct estimation of the bar height. Numerical experiments are necessary to conduct in order to properly determine the empirical parameters for the accurate simulation of profile changes.
Detection of resistance genes (gyrA,qepA,drf1,drf17) for E.coli in Iraqi aquatic environment ( symbols for genes are italicized (e.g., IGF1) in all the text
Authors: Mahdi Zinah Mohammed , Mahmood Shahad Husham , Baqer Noor Nihad 
Published: July 2023

The control of water represents the safe key for fair and optimal use to protect water resources due to human activities, including untreated wastewater, which is considered a carrier of a large number of antibiotic-resistant bacterial species. This study aimed to investigate the prevalence of antibiotic-resistance to E. coli in Tigris River by the presence of resistance genes for aminoglycoside(qepA( ,quinolone (gyrA), and sulfa drugs( dfr1 ,dfr17) due to the frequent use of antibiotics and their release into wastewater of hospitals. Samples were collected from three sites on Tigris River: S1( station wastewater in Adhamiya), S2 (station wastewater in Baghdad Medical city hospital), S3 (station wastewater in Abu Nuwas) from February-July 2021. Out of 67 isolates of bacteria, only 40 isolates of E. coli were detected by Vitek2. The antibiotic-resistance was estimated by the disk diffusion method. All E.coli isolates were tested against 6 antibiotics. The results showed the high resistance antibiotic of E. coli against Ceftazidime 70%, with intermediate resistance to Cefotaxime 47.5%, and low resistance to the sulfa drugs as Trimethoprim 27.5% and quinolones antibiotics as ciprofloxacin 17.5%, aminoglycosides as Amikacin and Gentamycin 5% and 7.5%. Moreover, the results revealed that gyrA gene was detected in 4 isolates (10%) while drf1 and drf17 genes were in 2 isolates of each gene (5%).Whereas qepA gene has not appeared in isolates. In conclusion, the isolates of E.coli from the Tigris River showed low resistance to sulfa drugs and quinolones , aminoglycosides. The resistance genes (gyrA,drf1,drf17) were detected in a few isolates which may be explained by the horizontal transfer of plasmids that carried genes and their distribution among the family Enterobacteriaceae.
The Mitochondrial Nucleoside Diphosphate Kinase NME4 Mediates Metabolic Reprogramming and Plays a Key Role in Nonalcoholic Fatty Liver Disease Progression
Authors: Li Xu , Xie shaofang , yuan lei , Sui Yue , Feng Shan , Li Hengle 
Published: July 2023

Abstract

Nonalcoholic fatty liver disease (NAFLD) is mainly characterized by excessive fat accumulation in the liver, and it is associated with liver-related complications and adverse systemic diseases. NAFLD has become the most prevalent liver disease; however, effective therapeutic agents for NAFLD are still lacking. We combined clinical data with proteomics and metabolomics data, and found that the mitochondrial nucleoside diphosphate kinase NME4 plays a central role in mitochondrial lipid metabolism. Nme4 is markedly upregulated in mice fed with high-fat diet, and its expression is positively correlated with the level of steatosis. Hepatic deletion of Nme4 suppresses the progression of hepatic steatosis. Further studies demonstrated that NME4 interacts with several key enzymes in coenzyme A metabolism and increases the level of acetyl-CoA and malonyl-CoA, which are the major lipid component of the liver in NAFLD. Increased acetyl-CoA and malonyl-CoA levels lead to increased triglyceride levels and lipid accumulation in the liver. Taken together, these findings reveal that NME4 is a critical regulator of NAFLD progression and a potential therapeutic target for NAFLD.
Abnormal theta oscillation in schizophrenic patients under auditory stimulation: a comparative study
Authors: Zhang Axue , Wei Chenyu , Wan Li 
Published: July 2023

Abstract
Background: There was growing evidences that theta (4-7Hz) power was highly likely to be an indicator of cognitive deficits in schizophrenia. And auditory information processing deficits in schizophrenia were reduced inhibition due to the disruption of the inhibitory mechanisms required to suppress redundant acoustic input. Further assuming that this inhibition was mainly evident in the theta band activity and reflected in the different brain regions.
Method: We chose to use the auditory paradigms to study the change of the theta band power in different brain regions in patients with patients using frequency domain analysis.
Results: The results showed that the sounds always induced abnormal elevation of theta oscillations, and when the frequency of sound stimulation was different, the striking elevation of the induced theta oscillations was reflected in temporal, parietal and central regions. While compared to the other frequencies, the abnormality of theta oscillations had specificity and stability.
Conclusion: This suggested that theta oscillations could serve as a biomarker in schizophrenia research. Future studies in the corresponding brain regions could also provide reference for the selection of the frequency and location for non-invasive treatment in patients with schizophrenia.
Shock Index Creatinine: A New Predictor of Mortality in Acute Coronary Syndrome Patients
Authors: Shariefuddin Widuri , Pramudyo Miftah , Martha Januar 
Published: July 2023

Abstract
Background The Shock Index Creatinine (SIC) scoring is a recently developed tool for risk stratification patients. These updated scoring was already used in ST-Elevation Myocardial Infarction (STEMI) patients. However its utility in predicting outcomes for patients with Acute Coronary Syndrome (ACS) remains unclear. This study aims to evaluate and update the current SIC score to predict in-hospital mortality among patients with ACS.Patients and methods: A retrospective cohort, Single-centered study enrolled 1393 ACS patients aged ≥ 18 years old diagnosed with ACS was conducted between January 2018 to January 2022. Study subjects were analyzed for in-hospital mortality and evaluated using binary linear regression analysis. The area under the curve (AUC) of SIC score was obtain to predict the sensitivity and specificity.Results Multivariate analysis showed that SIC score was significantly associated with in-hospital mortality. High SIC score (SIC ≥ 25) had significantly higher in-hospital mortality (p < 0.001) with odds ratio for (95% CIs) were 2.655 (1.6–4.31). Receiver operating characteristics (ROC) curve analysis determine the predictive power of SIC score for in-hospital mortality. SIC had an acceptable predictive value for in-hospital mortality (AUC = 0.789, 95% CI: 0.748–0.831, p < 0.001). The SIC score for sensitivity and specificity were, respectively, 71.5% and 74.4%, with optimal cutoff of SIC ≥ 25.Conclusion SIC had acceptable predictive value for in-hospital mortality in patients with all ACS spectrums. SIC was a useful parameter for predicting in-hospital mortality, particularly with a score ≥ 25. This is the first study to evaluate SIC in all spectrums of ACS.
Effect of thyroid function on periodontitis in U.S. population
Authors: han Wang , mingjun Zhang , Li nannan , miao Xiao , ka Chen , abulikemu Palizi , hongli Shi 
Published: July 2023

Abstract

Background
Thyroid dysfunction can lead to bone metabolism disorders and osteoporosis. Periodontitis is the most common oral disease, with alveolar bone resorption destruction as one of the main characteristics, and is associated with a variety of systemic diseases. Clinical and animal studies have shown that people with thyroid disorders are also at higher risk for osteoporosis. The progression of periodontitis may be accelerated by thyroid dysfunction because it may influence immune factor production and bone metabolism, which in turn inhibits alveolar osteogenesis and promotes bone resorption. This study analyze the correlative linkages between periodontitis and thyroid function.
Methods
This study used public data in two cycles of NHANES(2009–2010 and 2011–2012) for analysis. Participants were split into two groups on the basis of their status of periodontal condition: those without periodontitis and those who had it. We looked into the connection between periodontitis and thyroid function using a weighted logistic regression model. Then, using subgroup analysis, the potential contributing factors were explored.
Results
A total of 2352 subjects were included, and multiple regression models showed that after adjusting for all covariates, the low TSH level group was significantly associated with a 0.1-unit increase in periodontitis (adjusted for OR 1.48, 95% CI 1.01–2.17; p for trend = 0.048). According to the findings of the subgroup analysis, relationships between the two is remarkable despite variations in sex, age, vitamin D levels, and smoking status.
Conclusion
Periodontitis and thyroid function are tightly coupled, and that the onset of periodontitis is highly correlated with low TSH levels.
Clinical relevance: Patients with low serum TSH levels are more likely to have periodontitis. Stomatologists should focus more on thyroid disease patients, consider how systemic illnesses affect oral health, and enhance the standard of diagnosis and treatment.
On the Analysis of Physical Properties of Thermointerfaces Based on Hexagonal Boron Nitride Nanostructures for Cooling the Electronic Component Base of Micro- and Nanoelectronics
Authors: Prokhorov D. А. , Zuev S. М. 
Published: July 2023

Abstract
An empirical study of the physical properties (thermal conductivity, thermal diffusivity, and density) of thermophysical interfaces based on a hexagonal boron nitride (h-BN) nanopowder lattice for cooling electronic component bases in micro- and nano-electronics has been conducted. The physical properties were determined using the laser flash method and the relative method. The potential of compressed nanoparticles of hexagonal boron nitride as a thermophysical interface without an adhesive agent has been described. This article also provides a comparison of the physical properties of other thermophysical interfaces that are widely used today.
Microcomputed tomographic evaluation of 6 NiTi files on the pericervical dentin and the smallest dentin thickness zones in mesial root canals of mandibular molars: An in vitro study
Authors: Morales María de las Nieves Pérez , Sánchez José Antonio González , Elmsmari Firas , Durán-Sindreu Fernando , Salmon Phil , Jaramillo David E. , Fernández Juan Gonzalo Olivieri 
Published: July 2023

Abstract
Objectives: The aim of this study was to evaluate six files on the pericervical dentin (PCD) and the smallest dentin thickness zones (SDTZ) in mesial root canals of mandibular molars.
Materials and Methods: Sixty mesial roots with 2 canals were scanned before and after instrumentation with WaveOne Gold (WG), Reciproc Blue (RB), TRUShape (TS), XP-endo Shaper (XP), iRace (IR), and TruNatomy (TN). Surface, volume, structure thickness, SMI, and centroids were evaluated at the PCD, and the changes in the SDTZ were quantified. The data obtained werecompared using Wilcoxon and ANOVA with a 5% significance level.
Results: XP and TN were similar in all the parameters (P >.05) at the PCD, but TN showed significant differences from WG, RB, TS, and IR (P <.05), while XP showed significant differences from WG (P <.05) in volume, surface, and structure thickness. Regarding the changes in the SDTZ, the amount of dentin removed was similar between the groups in both canals at the middle 1/3, at the cervical 1/3 for MB canals, and in ML canals for RB, TS, XP, IR, and TN (P>.05). The action of WG was significantly different from that of XP and TN in the cervical 1/3 of the ML canal (P <.05).
Conclusions: XP and TN maintained better the anatomy, with minor changes at the PCD and SDTZ, while WG produced the largest changes. Centering at the PCD was maintained with all the files, and their performances were safe without risk of perforation.
Clinical Relevance: The choice of endodontic files is a relevant factor in the conservative performance of root canal treatments.
Trial Registration: No clinical trials were indicated in this study
Detecting Illegal Mining Activities in Zimbabwe through Earth Observation: A Case of Beitbridge District in Zimbabwe
Authors: Matingo Kumbirai 
Published: July 2023

Abstract
The Zimbabwean economy heavily relies on agricultural and mining activities which are the major boosters of the market and common business for regional and international trade [1]. These two sectors have seen a lot of investment potential and rise over the years and as such, illegal activities have since been on the rise in the mining industry. Across the country, most of the free, state land has become the breeding grounds for illegal mining activities, and this has caused quite several devastating effects and damage to the environment which have also contributed immensely to an uneven balance of biodiversity in the country [2]. The government on the other hand, has made various calls to action through its law enforcement agency to curb and spread of such illegal activities which pose a significant danger to both the environment and the economy [3]. Unfortunately, many of these efforts have been to no avail as many of these activities are going undetected leaving the law enforcement agencies together with the environmental protection agencies and partners without a starting point for countermeasures against such activities. Through Earth Observation technology and data, the government of Zimbabwe can be able to detect illegal mining activities and be in a better position to draft countermeasures which assist in curbing this national problem. On the other hand, Environmental Protection and Management agencies in Zimbabwe can be able to assess the damage caused by such activities among others for the benefit of crafting a sustainable future which leads to a balanced ecosystem which supports and promotes biodiversity at all levels. This paper provides a short case study of earth observation technology, techniques, and data applications in one of the well-known and well-assumed illegal mining hotspot towns of the country. It serves as a demonstration of how such technologies can be leveraged for development, enforcement and governmental approaches to issues affecting the environment and the future of today and tomorrow. The paper also gives a quantitative study and analysis of the damage that has been made by such illegal mining activities in the study area.