bluuebunny/crossref_metadata_2025_split huggingface dataset:
The brassinosteroid‐responsive protein OCTOPUS is a novel regulator of Arabidopsis thaliana immune signaling
Authors: Greenwood Kaitlyn N. , King Courtney L. , Melena Isabella , Stegemann Katherine A. , Donnelly Maura , Childers Anna , Mozal Raegan , Collins Carina A. , Spears Benjamin J. 
Published: August 2023

AbstractPhloem is a critical tissue for transport of photosynthates and extracellular signals in vascular plants. However, it also represents an ideal environment for pathogens seeking access to valuable host nutrients. Although many vascular pathogens induce economically relevant crop damage, there is still little known about the mechanisms by which immune signaling operates through the phloem. An existing phosphoproteomic dataset was mined to identify proteins that were both phosphorylated in response to the defense‐elicitor flagellin (flg22) and expressed in vascular cells. A single candidate, OCTOPUS (OPS), is polarly associated with the plasma membrane of sieve element cells and has been characterized as an inhibitor of brassinosteroid insensitive‐2 in promotion of brassinosteroid‐related phytohormone signaling. The observation that OPS is differentially phosphorylated in response to flg22 led us to the examine whether OPS may also regulate flg22‐induced immune signaling. Two independent alleles of ops exhibited enhanced immunity outputs across multiple signaling branches of PAMP‐triggered immunity (PTI), constitutively and in response to flg22 treatment. Together with our observation that interactions between OPS and brassinosteroid insensitive‐2 were disrupted by induction of salicylic acid and depletion of brassinosteriod, these data support a model whereby OPS modulates brassinolide and immune signaling to control downstream responses. We present OPS as a novel addition to the list of proteins with documented roles in PAMP‐PTI signaling. These results further indicate that immune signaling in the phloem may be a significant and unique component of the host detection and response to pathogens in vascular plants.
Combinations of essential oils as effective green stabilizers for single base propellants
Authors: Langendorf da Silva José Daniel , Rodrigues Rodrigo Leonard Barboza , Rosato Rogério , Lemos Maurício Ferrapontoff , Filho Letivan Mendonça , França Tanos Celmar Costa , Peixoto Fernando Cunha 
Published: August 2023

AbstractNitrocellulose‐based propellants undergo natural (and inevitable) autocatalytic degradation reactions. The presence of a stabilizer is essential to avoid and reduce the degradation reactions by capturing the NOx molecules. Traditional stabilizers, such as diphenylamine, and ethylcentralite, react with NOx through their amine and/or amide groups, leading to n‐nitroso groups, which are known to have carcinogenic, mutagenic and reprotoxic properties. Molecules that are not prone to form by‐products containing nitrosamines have been studied as substitutes, such as guaiacol and curcumin. However, the aromatic rings on these molecules have their acidity augmented when capturing the NOx groups and, paradoxically, increasing hydrolysis rates. In order to find a solution for the problem, we evaluated the use of essential oils containing terpenes and/or phenolic compounds to introduce a buffering capacity. Several propellant formulations with those compounds were subjected to traditional stability tests (German, Bergman–Junk and Storage tests), Pressure Vacuum Stability Test and Heat Flow Microcalorimetry. Finally, industrial batches were produced for the evaluation of real (ballistic) performance. Results indicated that the usage of essential oils increases stability properties not only through a sum of effects, but also in a synergistic way. Ballistic results were also adequate.
Combination of DNA methylation biomarkers with multiparametric magnetic resonance and ultrasound imaging fusion biopsy to detect the local spread of prostate cancer
Authors: Matulevičius Augustinas , Žukauskaitė Kristina , Gineikaitė Rugilė , Dasevičius Darius , Trakymas Mantas , Naruševičiūtė Ieva , Ušinskienė Jurgita , Ulys Albertas , Jankevičius Feliksas , Jarmalaitė Sonata 
Published: August 2023

AbstractBackgroundThis study aimed to investigate the extent of field cancerization adjacent to index lesions in prostate cancer (PCa) by measuring DNA methylation of selected tumor suppressor genes in the perifocal tissue of PCa not visible on multiparametric magnetic resonanse imaging (mpMRI) for the safe zone of focal therapy identification.MethodsA total of 272 patients were enrolled in this study, 44 patients’ tissue biosamples were included in the field cancerization research, and 272 urine samples were included in the urine‐based test development. Targeted biopsies were performed using the mpMRI/ultrasoundimage fusion system.ResultsQuantitative analysis revealed significantly higher DNA methylation levels of RARB, RASSF1, GSTP1 & APC genes in the index lesion compared with perifocal tissue samples 10 mm away from it (p < 0.0001). Notably, the RARB, GSTP1 & APC and RARB, RASSF1, GSTP1 & APC biomarker combinations exhibited the highest sensitivity and specificity comparing the extent of DNA methylation in index lesions and noncancerous prostate tissues 20 mm away (both area under the curve [AUC] = 0.98; p < 0.0001). The analysis of the potential urinary biomarkers showed that the combination of all four DNA methylation biomarkers with prostate‐specific antigen (PSA) or PSA density (PSAD) in the blood significantly improves the detection of clinically significant PCa (csPCa). The combination of the four‐biomarker test with PSAD allowed the identification of csPCa with ≥90% sensitivity and specificity.ConclusionThus, this study suggests that for focal therapy by region target hemi‐ablation, the safe distance from the index lesion is no less than 10 mm. Noninvasive urine DNA methylation tests in combination with PSAD could be used for further follow‐up of the patients, but larger prospective studies with external validation are needed.
Mechanistic understanding of bacterial FAALs and the role of their homologs in eukaryotes
Authors: Mondal Sudipta , Pal Biswajit , Sankaranarayanan Rajan 
Published: August 2023

AbstractFatty acids are used in fundamental cellular processes, such as membrane biogenesis, energy generation, post‐translational modification of proteins, and so forth. These processes require the activation of fatty acids by adenosine triphosphate (ATP), followed by condensation with coenzyme‐A (CoA), catalyzed by the omnipresent enzyme called Fatty acyl‐CoA ligases (FACLs). However, Fatty acyl‐AMP ligases (FAALs), the structural homologs of FACLs, operate in an unprecedented CoA‐independent manner. FAALs transfer fatty acids to the acyl carrier protein (ACP) domain of polyketide synthases (PKS) and non‐ribosomal peptide synthetases (NRPS) for the biosynthesis of various antibiotics, lipopeptides, virulent complex lipids, and so forth in bacteria. Recent structural and biochemical insights from our group provide a detailed understanding of the mode of CoA rejection and ACP acceptance by FAALs. In this review, we have discussed advances in the mechanistic, evolutionary, and functional understanding of FAALs and FAAL‐like domains across life forms. Here, we are proposing a “Five‐tier” mechanistic model to explain the specificity of FAALs. We further demonstrate how FAAL‐like domains have been repurposed into a new family of proteins in eukaryotes with a novel function in lipid metabolism.
Suspected oncologic adverse reactions associated with interleukin‐23 inhibitors in EudraVigilance: Comparative study and gender distribution
Authors: Calapai Fabrizio , Mannucci Carmen , Cardia Luigi , Currò Mariaconcetta , Calapai Gioacchino , Esposito Emanuela , Ammendolia Ilaria 
Published: August 2023

AbstractPsoriasis is a chronic inflammatory skin disease characterized by plaque formation. Interleukin (IL)‐23 is upregulated in psoriatic lesions and is thought to be a major regulator of the Th17 pathway in psoriasis pathogenesis. Three monoclonal antibodies targeting the IL‐23p19 subunit, guselkumab, tildrakizumab, and risankizumab, have been approved for psoriasis therapy. The balance between cytokines IL‐23 and IL‐12 can affect antitumor and pro‐tumor immune activities, and patients with psoriasis may have higher rates of cancer than the general population. Moreover, a chronic inflammatory state typical of psoriasis may induce protumorigenic effects, however, the potential risk of malignancy in patients taking these drugs remains largely unknown. This study investigated the occurrence of malignancies as suspected adverse reactions (SARs) potentially associated with IL‐23 inhibitors by analyzing real‐world data from the European EudraVigilance database. Although indicatory, these real‐world data seem to confirm the potential association between the IL‐23 inhibitors risankizumab and tildrakizumab, and the occurrence of SARs linked to cancer in patients with psoriasis and, according to a gender perspective, they show that this relationship is asymmetrically distributed between women and men, with a clear prevalence of oncologic SARs in men.
Adaptive governance by community based organisations: Community resilience initiatives during Covid‐19 in Mathare, Nairobi
Authors: Fransen Jan , Hati Beatrice , Simon Harrison Kioko , van Stapele Naomi 
Published: August 2023

AbstractAdaptive governance describes the purposeful collective actions to resist, adapt, or transform when faced with shocks. As governments are reluctant to intervene in informal settlements, community based organisations (CBOs) self‐organize and take the lead. This study explores under what conditions CBOs in Mathare informal settlement, Nairobi initiate and sustain resilience activities during Covid‐19. Study findings show that CBOs engage in multiple resilience activities, varying from maladaptive and unsustainable to adaptive, and transformative. Two conditions enable CBOs to initiate resilience activities: bonding within the community and coordination with other actors. To sustain these activities over 2.5 years of Covid‐19, CBOs also require leadership, resources, organisational capacity, and network capacity. The same conditions appear to enable CBOs to engage in transformative activities. However, CBOs cannot transform urban systems on their own. An additional condition, not met in Mathare, is that governments, NGOs, and donor agencies facilitate, support, and build community capacities.
Going green for good: How sustainable economic development and green energy resources are driving carbon neutrality in G‐7 countries?
Authors: Mohsin Muhammad , Xuhua Hu , Sarfraz Muddassar , Naseem Sobia 
Published: August 2023

AbstractThe construction of a sustainable society in the present era is significantly based on green economic development, environmental sustainability, and ecological energy resources. It is now more difficult than ever for economists, environmentalists, and policymakers to achieve sustainable environmental development and economic growth while preventing environmental damage enhance the burden on the economy. It is worth mentioning that this research approach is leading CO2 emission resources in G‐7 countries as dependent variables and possibly CO2‐reducing determinants as independent variables from 1993 to 2020. The research data are extracted from the world development indicators, and these are secondary nature data. The stationarity of the data series is checked by employing a panel unit root and proceeds to the cointegration test. The cointegration, fully modified ordinary least squares (FMOLS), quantile regression, and wavelet coherence tests have confirmed the long‐run relationship among variables. Renewable energy and economic growth are aidant factors in reducing CO2 emissions from all resources. Low carbon energy is reduced CO2 from coal and gas while increasing the CO2 emission from oil. Renewable energy, low carbon energy, and rapid growth of gross domestic product (GDP) are environmentally friendly factors which can eliminate gas energy emissions. This research presents practical policy implications that address globalization trends, carbon emissions, and the overall energy structure and economic growth of G‐7 countries.
Uncovering dynamic complexity in annual reports: a methodological approach using resource mapping
Authors: Kunc Martin , Barnabè Federico , Giorgino Maria Cleofe 
Published: August 2023

AbstractThis study explores the role of qualitative system dynamics (SD) models in representing and analyzing the information of corporate annual reports by uncovering their hidden “dynamic complexity.” The study employs a specific qualitative SD technique, resource mapping, and outlines a methodology to apply it in practice. This study has several contributions. First, it provides methodological guidelines and practical insights on how to apply qualitative SD, using stock‐and‐flow diagrams, in the field of corporate reporting to represent visually and analyze the dynamic complexity implicit in businesses. Second, it underlines the performative role of accounting together with qualitative SD. Specifically, it provides useful insights into how to use qualitative SD in the accounting field to enhance both internal analysis and external communication, thereby supporting decision‐making processes. Third, it shows how to integrate different discipline‐related technical languages, thereby bridging differences in backgrounds, skills, and expertise that might characterize intended readers and users. © 2023 The Authors. System Dynamics Review published by John Wiley & Sons Ltd on behalf of System Dynamics Society.
Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges, and Perspectives
Authors: Yin Weihong , Zhuang Jianjian , Li Jiale , Xia Liping , Hu Kai , Yin Juxin , Mu Ying 
Published: August 2023

AbstractDigital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis. Recently, isothermal amplification technologies such as recombinase polymerase amplification (RPA), loop‐mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats‐CRISPR associated proteins (CRISPR‐Cas) assisted technologies have become a hot spot of attention and state‐of‐the‐art digital nucleic acid chips have provided a powerful tool for these technologies. Herein, isothermal amplification technologies including RPA, LAMP, and CRISPR‐Cas assisted methods, based on digital nucleic acid microfluidics chips recently, have been reviewed. Moreover, the challenges of digital isothermal amplification and possible strategies to address them are discussed. Finally, future directions of digital isothermal amplification technology, such as microfluidic chip and device manufacturing, multiplex detection, and one‐pot detection, are outlined.
Photoelectrochemical Engineering for Light‐Assisted Rechargeable Metal Batteries: Mechanism, Development, and Future
Authors: Bao Weizhai , Wang Ronghao , Liu Hongmin , Qian Chengfei , Liu He , Yu Feng , Guo Cong , Li Jingfa , Sun Kaiwen 
Published: August 2023

AbstractRechargeable battery devices with high energy density are highly demanded by our modern society. The use of metal anodes is extremely attractive for future rechargeable battery devices. However, the notorious metal dendritic and instability of solid electrolyte interface issues pose a series of challenges for metal anodes. Recently, considering the indigestible dynamical behavior of metal anodes, photoelectrochemical engineering of light‐assisted metal anodes have been rapidly developed since they efficiently utilize the integration and synergy of oriented crystal engineering and photocatalysis engineering, which provided a potential way to unlock the interface electrochemical mechanism and deposition reaction kinetics of metal anodes. This review starts with the fundamentals of photoelectrochemical engineering and follows with the state‐of‐art advance of photoelectrochemical engineering for light‐assisted rechargeable metal batteries where photoelectrode materials, working principles, types, and practical applications are explained. The last section summarizes the major challenges and some invigorating perspectives for future research on light‐assisted rechargeable metal batteries.
Structural and Interfacial Manipulation of Multifunctional Skeletons Enabled Shuttling‐Free and Dendrite‐Free Li–S Full Batteries
Authors: Zhang Miaomiao , Luo Rongjie , Guo Qifei , Tang Zihuan , Li Xingxing , Gao Biao , Zhang Xuming , Huo Kaifu , Zheng Yang 
Published: August 2023

AbstractLi–S batteries are regarded as promising devices for energy storage systems owing to high energy density, low cost, and environmental friendliness. However, challenges of polysulfides shuttling in sulfur cathode and dendrite growth of lithium anode severely hinder the practical application. Developing advanced skeletons simultaneously regulating the cathode and anode is significant and challenging. Hence, a novel and scalable strategy combining spray drying and topological nitriding is proposed, and hierarchically assembled rGO hollow microspheres encapsulated highly porous nanospheres consisted of ultrafine Nb4N5‐Nb2O5 or Nb4N5 nanoparticles as multifunctional skeletons for S and Li are designed. In such unique architecture, a 3D highly porous structure provides abundant void space for loading of S and Li, and accommodates volume change during cycling. Moreover, Nb4N5‐Nb2O5 heterostructured interface promotes adsorption‐conversion process of polysulfides, while strong lithophilic Nb4N5 induces the selective infiltration of Li into the void of the skeleton and regulates the uniform deposition and growth. More interestingly, in situ generated Li3N@Nb ion/electron conducting interfaces induced by the reaction of Nb4N5 and Li reduce the nucleation overpotential and induce selective deposition of Li into the cavity. Consequently, the Li–S full cell exhibits superior cycling stability and impressive rate performance with a low capacity ratio of negative/positive.
Nucleation and Growth of Nanocrystals Investigated by In Situ Transmission Electron Microscopy
Authors: Ye Huanyu , Zhang Zhihong , Wang Rongming 
Published: August 2023

AbstractNanocrystals play a key role in the modern energy, catalysis, semiconductor, and biology industries due to their unique structures and performances. However, controllable fabrication of ideal nanocrystals with the desired structures and properties is still challenging, which needs a deep understanding of their nucleation and growth process. Here, the research on nucleation and growth of nanocrystals studied by in situ transmission electron microscopy (TEM) is reviewed, mainly focusing on the atomic migration dynamics, interface evolution, and structure transformation. In addition, the challenges in the study of nanocrystal growth by TEM are discussed and the perspective on the future development of advanced in situ TEM techniques is provided. It is hoped that the review can give a deep insight into the nanocrystal nucleation and growth process, and further contribute to the rational design and precise fabrication of high‐performance functional nanocrystals.
Hierarchical MoN@NiFe‐LDH Heterostructure Nanowire Array for Highly Efficient Electrocatalytic Hydrogen Evolution
Authors: Dang Yujian , Li Xu , Chen Zekun , Zhao Xudong , Ma Bo , Chen Yantao 
Published: August 2023

AbstractThe slow charge transfer and high energy barrier are the key restrictions of cost‐effective electrocatalysts for hydrogen production. A hierarchical heterostructure of MoN@NiFe‐layered double hydroxides (LDH) is developed, with NiFe‐LDH nanosheets supported on MoN nanowire arrays. The as‐prepared MoN@NiFe‐LDH exhibits a remarkably high performance on hydrogen production in alkaline medium, which is close to the benchmark Pt/C. The theoretical computations indicate that MoN@NiFe‐LDH has a metallic character inherited from MoN, which gives rise to the promoted charge transfer. Furthermore, the adsorption intensity of intermediates on MoN@NiFe‐LDH is optimized and thereby the energy barrier is diminished. This work demonstrates the significance of constructing heterostructure for boosting the charge transfer and reducing the energy barrier, which can shed light on the development of highly efficient and low‐cost electrocatalyst for hydrogen production.
Green Solvent Selection for All Solution‐Processed Inverted Quantum Dot Light Emitting Diode
Authors: Kim Hyo‐Bin , Yoo Jae‐In , Kang Sung‐Cheon , Song Jang‐Kun 
Published: August 2023

AbstractQuantum‐dot light‐emitting diodes (QD‐LEDs) have gained attention as potential display technologies. However, the solvents used to dissolve a polymeric hole transport layer (HTL) are hazardous to both humans and the environment. Additionally, intermixing the HTL and QD layers presents a significant challenge when fabricating inverted QD‐LEDs. Here, a green solvent selection procedure to achieve good device performance and environmental safety in QD‐LEDs is established. This procedure utilizes Hansen solubility parameters and surface roughness to identify a set of solvents that do not lower the device performance by avoiding interlayer mixing or a rough interface. The CHEM21 solvent selection guide is used to screen for environmentally hazardous solvents. Finally, cyclopentanone (CPO) is selected as the optimal HTL solvent from among 16 candidates. Using CPO improves the maximum luminescence by ≈1.6 times and the maximum current efficiency by ≈12.6 times, compared to that of conventional devices using hazardous chlorobenzene. Solvent selection is critical for the fabrication of green and high‐performance inverted QD‐LEDs, particularly for large display panels that require n‐type oxide thin‐film transistors.
Biomimetic Metal‐Pyrimidine Nanoflowers: Enzyme Immobilization Platforms with Boosted Activity
Authors: Gong Changbao , Wang Denghao , Zhao Huimin 
Published: August 2023

AbstractFor the enzyme immobilization platform, enhancing enzyme activity retention while improving enzyme stability remains a challenge for sensitive sensing analysis. Herein, an in situ biomimetic immobilized enzyme carrier (metal‐pyrimidine nanoflowers, MPNFs) synthesized by the coordination of DNA base derivative (2‐aminopyrimidine) with Zn2+ in the aqueous phase at room temperature is developed. The biocompatibility of 2‐aminopyrimidine and the hydrophilicity and green synthetic conditions of MPNFs allows the immobilized enzymes to retain above 91.2% catalytic activity. On this basis, a cascade catalytic platform is constructed by simultaneously immobilizing acetylcholinesterase (AChE), choline oxidase (CHO), and horseradish peroxidase (HRP) in MPNFs (AChE/CHO/HRP@MPNFs) for organophosphorus pesticides (OPs) colorimetric biosensing detection. The assay could specifically detect parathion‐methyl within 13 min with a wider linear range (0.1–1000.0 nm) and a lower limit of detection (LOD) (0.032 nm). The remarkable stability of the immobilized enzymes is also achieved under harsh environments, room temperature storage, and recycling. Furthermore, a portable and cost‐effective biosensing platform is developed by integrating AChE/CHO/HRP@MPNFs with a smartphone‐assisted paper device for the on‐site detection of OPs. Overall, the high catalytic activity retention and the enhanced detection performance demonstrate that MPNF is a robust carrier in enzyme immobilization and holds great promise in biosensing and other field applications.