bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calpain-3 not only proteolyzes calpain-1 and -2 but also is a substrate for calpain-1 and -2
Authors: Ojima Koichi , Hata Shoji , Shinkai-Ouchi Fumiko , Ono Yasuko , Muroya Susumu 
Published: July 2023

Abstract
Calpain is an intracellular cysteine protease that cleaves its specific substrates in a limited region to modulate cellular function. Calpain-1 (C1) and calpain-2 (C2) are ubiquitously expressed in mammalian cells, but calpain-3 (C3) is a skeletal muscle-specific type. In the course of calpain activation, the N-terminal regions of all three isoforms are clipped off in an intramolecular or intermolecular fashion. C1 proteolyzes C2 to promote further proteolysis, but C2 proteolyzes C1 to suspend C1 proteolysis, indicating the presence of C1–C2 reciprocal proteolysis. However, whether C3 is involved in the calpain proteolysis network is unclear. To address this, we examined whether GFP-tagged C3:C129S (GFP-C3:CS), an inactive protease form of C3, was a substrate for C1 or C2 in HEK cells. Intriguingly, the N-terminal region of C3:CS was cleaved by C1 and C2 at the site identical to that of the C3 autoproteolysis site. Furthermore, the N-terminal clipping of C3:CS by C1 and C2 was observed in mouse skeletal muscle lysates. Meanwhile, C3 preferentially cleaved the N-terminus of C1 over that of C2, and the sizes of these cleaved proteins were identical to their autoproteolysis forms. Our findings suggest an elaborate inter-calpain network to prime and suppress proteolysis of other calpains.
Recent Developments in the National Implementation of Biological Weapons Convention: What Happened Since Resolution 1540?
Authors: de Vries Barry 
Published: July 2023

Abstract
The national implementation of the Biological Weapons Convention (BWC) has long been a point of discussion. One aspect of this national implementation concerns the enactment of penal legislation addressing the prohibitions of the BWC. There has been a clear increase in the past 18 years of penal legislation addressing biological weapons. This coincides with the adoption of UN Security Council Resolution 1540. Through a comprehensive assessment of the adopted national legislation it is possible to see trends and consider the extent to which such legislation seeks to address the BWC or Resolution 1540. The assessment clearly indicates that there is a divergence between the legislation addressing biological weapons in a general manner and legislation specifically seeking to address biological weapons in a terrorism context. This analysis will recognize potential avenues to increase national implementation of the BWC by looking at the progress made in the context of Resolution 1540.
Crimes without Humanity?
Authors: Acquaviva Guido 
Published: July 2023

Abstract
The development of autonomous weapons systems (AWS) and, more generally, the role of artificial intelligence in warfare, may come to pose unprecedented challenges to criminal law, including by making it harder to link harm to individuals who can be held responsible, due to the pivotal role of the concepts of actus reus, mens rea and causation in that domain. In this context, the notion of meaningful human control has been proposed to address some of the challenges of ensuring accountability for serious violations of international humanitarian law. One possibility might be to link — conceptually, or even legally — meaningful human control with the ‘control theory’ propounded at the International Criminal Court to assign criminal responsibility. Under this theory, the ascription of criminal responsibility to an individual as a direct perpetrator requires an assessment of whether they enjoy an effective ability to decide on the commission of a crime. This article elaborates on some of the issues posed by this approach, proceeding then to consider the most ‘extreme’ instance of AWS, i.e. the deployment of swarms of drones operating autonomously and coordinating their behaviour in a decentralized manner.
The Role of Intermediate Collaborative Forums in Polycentric Environmental Governance
Authors: Wiechman Adam , Alonso Vicario Sara , Koebele Elizabeth A 
Published: July 2023

Abstract
In complex, polycentric environmental governance systems, actors may choose to collaborate with one another to reduce their collective vulnerability and enhance system function. However, collaboration can be costly, and little evidence exists for how particular collaborative forums impact the broader governance system in which they are embedded. To address this gap, we investigate the role of intermediate collaborative forums, which support collaboration among a subset of system actors, in polycentric governance systems. Empirically, we analyze the structural and functional role of an intermediate collaborative forum called the Arizona Municipal Water Users Association (AMWUA) within the municipal surface water governance network for the Phoenix Metropolitan Area (PMA) in Arizona, United States. To do this, we draw from 21 interviews with water professionals in the PMA, which we analyze through a combination of network analysis and qualitative coding. We find that AMWUA facilitates strong bonding capacities among members, allowing for streamlined bridging to the rest of the network that enhances information processing and advocacy of member needs. Our findings advance theory on the role of collaboration in polycentric systems and inform the design of collaborative institutions to improve environmental governance.
Rethinking encounter through parochial meaning-making on the urban margins in South Africa
Authors: Monson Tamlyn 
Published: July 2023

Abstract
Originating in normative interventions aiming to remedy segregation through contact with difference, the notion of ‘meaningful encounter’ has become a portmanteau for contact that results in greater respect and tolerance. This article questions the epistemic justice of such a value-laden concept by presenting four examples of parochial meaning-making around encounters in a South African informal settlement. Drawing on secondary interviews conducted after ‘xenophobic’ expulsions of foreign-born newcomers from the settlement in 2008, as well as primary ethnographic fieldwork conducted four years later, the article highlights the meaningfulness of four encounters that did not lead to increased tolerance or respect for difference—encounters with affluence; with social mobility; with hardship in conditions of poverty and marginality; and with seemingly uncommitted newcomers against a backdrop of contentious politics. In each case, the article illustrates both the meaningfulness of the encounter, and its effect of producing tensions rather than mediating them. The article uses these examples to problematise the assumptions implicit in received notions of ‘meaningful encounter’: that tension necessarily pre-exist encounter, that established/newcomer boundaries are based on prejudice, and that encounters are not meaningful unless they advance a predetermined normative position. Along the way, it highlights the importance of embodiment, materiality, inequality, and space in the production of difference. Having made the case for a conceptual sharpening in the light of these seldom-studied encounters in the urban margins of South Africa, the article concludes by urging scholars to adopt a value-free conception of the meaningfulness of encounter.
Constraints on galaxy formation from the cosmic-far-infrared-background – optical-imaging cross-correlation using Herschel and UNIONS
Authors: Lim Seunghwan , Hill Ryley , Scott Douglas , van Waerbeke Ludovic , Cuillandre Jean-Charles , Carlberg Raymond G , Chisari Nora Elisa , Dvornik Andrej , Erben Thomas , Gwyn Stephen , McConnachie Alan W , Miville-Deschênes Marc-Antoine , Wright Angus H , Duc Pierre-Alain 
Published: July 2023

ABSTRACT
Using Herschel-SPIRE imaging and the Canada-France Imaging Survey (CFIS) Low Surface Brightness data products from the Ultraviolet Near-Infrared Optical Northern Survey (UNIONS), we present a cross-correlation between the cosmic far-infrared background and cosmic optical background fluctuations. The cross-spectrum is measured for two cases: all galaxies are kept in the images; or all individually detected galaxies are masked to produce ‘background’ maps. We report the detection of the cross-correlation signal at $\gtrsim 18\, \sigma$ ($\gtrsim 14\, \sigma$ for the background map). The part of the optical brightness variations that are correlated with the submm emission translates to an rms brightness of $\simeq 32.5\, {\rm mag}\, {\rm arcsec}^{-2}$ in the r band, a level normally unreachable for individual sources. A critical issue is determining what fraction of the cross-power spectrum might be caused by emission from Galactic cirrus. For one of the fields, the Galactic contamination is 10 times higher than the extragalactic signal; however, for the other fields, the contamination is around 20 per cent. An additional discriminant is that the cross-power spectrum is of the approximate form P(k) ∝ 1/k, much shallower than that of Galactic cirrus. We interpret the results in a halo-model framework, which shows good agreement with independent measurements for the scalings of star-formation rates in galaxies. The approach presented in this study holds great promise for future surveys such as FYST/CCAT-prime combined with Euclid or the Vera Rubin Observatory (LSST), which will enable a detailed exploration of the evolution of star formation in galaxies.
Cosmological constraints with the linear point from the BOSS survey
Authors: He Mengfan , Zhao Cheng , Shan Huanyuan 
Published: July 2023

ABSTRACT
The Linear Point (LP), defined as the mid-point between the baryon acoustic oscillation (BAO) peak and the associated left dip of the two-point correlation function (2PCF), ξ(s), is proposed as a new standard ruler which is insensitive to non-linear effects. In this paper, we use a Bayesian sampler to measure the LP and estimate the corresponding statistical uncertainty, and then perform cosmological parameter constraints with LP measurements. Using the Patchy mock catalogues, we find that the measured LPs are consistent with theoretical predictions at 0.6 per cent level. We find constraints with mid-points identified from the rescaled 2PCF (s2ξ) more robust than those from the traditional LP based on ξ, as the BAO peak is not always prominent when scanning the cosmological parameter space, with the cost of 2–4 per cent increase of statistical uncertainty. This problem can also be solved by an additional data set that provides strong parameter constraints. Measuring LP from the reconstructed data slightly increases the systematic error but significantly reduces the statistical error, resulting in more accurate measurements. The 1 σ confidence interval of distance scale constraints from LP measurements are 20–30 per cent larger than those of the corresponding BAO measurements. For the reconstructed Sloan Digital Sky Survey Data Release 12 data, the constraints on H0 and Ωm in a flat-Lambda cold dark matter framework with the LP are generally consistent with those from BAO. When combined with Planck cosmic microwave background data, we obtain $H_0=68.02_{-0.37}^{+0.36}$ ${\rm km}\, {\rm s}^{-1}\, {\rm Mpc}^{-1}$ and $\Omega _{\rm m}=0.3055_{-0.0048}^{+0.0049}$ with the LP.
Impact of menopausal symptoms on presenteeism in Japanese women
Authors: Ishimaru T , Okawara M , Tateishi S , Yasui T , Horie S , Fujino Y 
Published: July 2023

Abstract

Background
Menopausal symptoms are common among middle-aged women. Working women with severe menopausal symptoms are more likely to experience presenteeism—a condition where employees continue to work despite feeling unwell. However, it remains unclear as to which specific symptoms women experience during the menopausal transition and postmenopausal periods that primarily contribute to presenteeism.

Aims
To evaluate the associations between types of menopausal symptoms and presenteeism among Japanese women.

Methods
A cross-sectional study of 4000 women aged 40–59 years who were currently working was conducted in Japan in September 2022. We used an online self-administered questionnaire that included items on demographic characteristics, the Menopause Rating Scale for measuring menopausal symptoms and the Work Functioning Impairment Scale for measuring presenteeism. Logistic regression analysis was performed.

Results
Women with severe overall menopausal symptoms had 12.18-fold (95% confidence interval [CI] 9.09–16.33, P &lt; 0.001) increased odds of presenteeism compared with those without symptoms. Participants with psychological symptoms also had significantly higher presenteeism (severe: odds ratio: 9.18, 95% CI 6.60–12.78, P &lt; 0.001). However, after controlling for psychological symptoms, there were no significant associations between somatic and urogenital symptoms and presenteeism.

Conclusions
The results indicate that menopausal symptoms, especially psychological symptoms, have a significant impact on presenteeism among Japanese women. Organizations need to address menopausal symptoms in the workplace, with an emphasis on reducing work-related stress for women with menopausal symptoms.
Role of Potassium-Dependent Alternative Splicing of MYB59 in the Maintenance of Potassium Concentration in Shoots of Arabidopsis thaliana
Authors: Enomoto Takuo , Tanaka Nobuhiro , Fujiwara Toru , Nishida Sho 
Published: July 2023

Abstract
Potassium (K) is a major plant nutrient. K+ is taken up by channel and transporter proteins in roots and translocated from roots to shoots via the xylem. In Arabidopsis thaliana, the K+ transporter NPF7.3 mediates K+ loading into the xylem and the transcription factor MYB59 is responsible for NPF7.3 expression. Here, we demonstrate that MYB59 is regulated by alternative splicing in response to K availability. Three splicing isoforms of MYB59 are detected in roots: an isoform with the first intron spliced out encodes a protein with the full DNA-binding motif (MYB59α), and two isoforms with the first intron retained partially or completely encode a protein missing part of the DNA-binding motif (MYB59β). Functional analysis showed that only MYB59α is capable of inducing the expression of NPF7.3. The abundance of the MYB59α isoform increased under low K, but the total abundance of MYB59 transcripts did not change, indicating that MYB59α is increased by modification of the splicing pattern in response to low K. Although MYB59α is increased by low K, NPF7.3 expression remained constant independent of K. In addition, there was no significant difference in NPF7.3 expression between an MYB59 knockout mutant and the wild type under normal K. These results suggest that an unknown mechanism is involved in NPF7.3 expression under normal K and switches roles with MYB59 under low K. We propose that the regulation of MYB59 by alternative splicing is required for the maintenance of shoot K concentration in adaptation to low K.
Homogenisation of Nonlinear Heterogeneous Thin Plate When the Plate Thickness and In-Plane Heterogeneities are of the Same Order of Magnitude
Authors: Pruchnicki E 
Published: July 2023

Summary
In this work, we propose a new two-scale finite-strain thin plate theory for highly heterogeneous plates described by a repetitive periodic microstructure. For this type of theory, two scales exist, the macroscopic one is linked to the entire plate and the microscopic one is linked to the size of the heterogeneity. We consider the case when the plate thickness is comparable to in-plane heterogeneities. We assume that the nonlinear macroscopic part of the model is of Kirchhoff–Love type. We obtain the nonlinear homogenised model by performing simultaneously both the homogenisation and the reduction of the initial three-dimensional plate problem to a two-dimensional one. Since nonlinear equations are difficult to solve, we linearise this homogenised Kirchhoff–Love plate theory. Finally, we discuss the treatment of edge effects in the vicinity of the lateral boundary of the plate.
Impact of COVID-19 infection experience on mental health status of intensive care unit patients’ family members: a real-world study
Authors: Liu T , Zhao Z , Wu C , Lu C , Liu M , An X , Sha Z , Wang X , Luo Z , Chen L , Liu C , Cao P , Zhang D , Jiang R 
Published: July 2023

Summary

Purpose
Family members of patients hospitalized in intensive care unit (ICU) are susceptible to adverse psychological outcomes. However, there is a paucity of studies specifically examining the mental health symptoms in ICU patients’ family members with a prior history of coronavirus disease 2019 (COVID-19) infection.

Aim
This study aimed to investigate mental health status and its influencing factors of ICU patients’ family members with previous COVID-19 infection experience in China.

Design
Nationwide, cross-sectional cohort of consecutive participants of family members of ICU patients from 10 provinces randomly selected in mainland China conducted between October 2022 and May 2023.

Methods
The basic information scale, Self-rating depression scale, Self-rating Anxiety Scale, Impact of Event Scale-Revised, Pittsburgh sleep quality index, Perceived Stress Scale, Connor-Davidson resilience scale, Simplified Coping Style Questionnaire were employed to explore mental health status among participants.

Results
A total of 463 participants, comprising 156 individuals in Covid-19 family member cohort (infection group) and 307 individuals in control family member cohort (control group), met inclusion criteria. The infection group exhibited significantly higher incidence of composite mental health symptoms compared to control group (P = 0.017). Multivariable logistic regression analysis revealed that being female, engaging in physical/mental labor, residing in rural areas, and having children were identified as risk factors for the development of depression, anxiety, and post-traumatic stress disorder symptoms, while medical history of surgery was protective factor. A predictive model demonstrated a favorable discriminative ability and excellent calibration.

Conclusion
COVID-19 infection experience regarded as new traumatic stressors worsen mental health status of ICU patients’ family members.
Prevalence and identification of neuropsychiatric symptoms in systemic autoimmune rheumatic diseases: an international mixed methods study
Authors: Sloan Melanie , Wincup Chris , Harwood Rupert , Pollak Thomas A , Massou Efhalia , Bosley Michael , Pitkanen Mervi , Zandi Michael S , Leschziner Guy , Barrere Colette , Ubhi Mandeep , Andreoli Laura , Brimicombe James , Diment Wendy , Jayne David , Gordon Caroline , Naughton Felix , D’Cruz David 
Published: July 2023

Abstract

Objective
A limited range of neuropsychiatric symptoms have been reported in systemic autoimmune rheumatic diseases (SARDs), with varied symptom prevalence. This study aimed to investigate a wider range of potential symptoms than previous studies, compare patient self-reports with clinician estimates, and explore barriers to symptom identification.

Methods
Mixed methods were used. Data from SARDs patients (n = 1853) were compared with controls (n = 463) and clinicians (n = 289). In-depth interviews (n = 113) were analysed thematically. Statistical tests compared means of survey items between patients and controls, 8 different SARD groups, and clinician specialities.

Results
Self-reported lifetime prevalences of all 30 neuropsychiatric symptoms investigated (including cognitive, sensorimotor and psychiatric) were significantly higher in SARDs than controls. Validated instruments assessed 55% of SARDs patients as currently having depression and 57% anxiety. Barriers to identifying neuropsychiatric symptoms included: (i) limits to knowledge, guidelines, objective tests and inter-speciality cooperation; (ii) subjectivity, invisibility and believability of symptoms; and (iii) under-eliciting, under-reporting and under-documenting. A lower proportion of clinicians (4%) reported never/rarely asking patients about mental health symptoms than the 74% of patients who reported never/rarely being asked in clinic (P &lt; 0.001). Over 50% of SARDs patients had never/rarely reported their mental health symptoms to clinicians, a proportion underestimated at &lt;10% by clinicians (P &lt; 0.001).

Conclusion
Neuropsychiatric symptom self-reported prevalences are significantly higher in SARDs than controls, and are greatly underestimated by most clinicians. Research relying on medical records and current guidelines is unlikely to accurately reflect patients’ experiences of neuropsychiatric symptoms. Improved inter-speciality communication and greater patient involvement is needed in SARD care and research.
Acute toxicity study, haematological, biochemical and histopathological effects of Brassica oleracea var. capitata, Allium porrum and Cucurbita maxima in Rattus norvegicus
Authors: Alhaji Saganuwan Saganuwan , Junior Atanu Samuel , Tsekohol Agu Solomon , Wanmi Nathaniel 
Published: July 2023

Abstract

Objectives
The use of vegetables in Nigeria as nutraceutical cannot be over emphasized. However many vegetables are associated with toxicity effects at both low and high doses.

Methods
Hence the study was aimed at investigating acute toxicity, haematological, biochemical and histopathological effects of Allium porrum (leek), Cucurbita maxima (pumpkin) and Brassica oleracea var. capitata (cabbage) in female Rattus norvegicus. The lethal dose50 (LD50) of the three plants were determined.

Key findings
The LD50 of aqueous extract of cabbage, leek and pumpkin was 6585 mg/kg body weight. Cabbage caused a decrease in packed cell volume, erythrocytes counts, haemoglobin concentration, white blood cells, neutrophils and monocytes counts, increased basophils and eosinophils counts at dose levels of 5.79 mg/kg and 57.93 mg/kg body weight. At dose levels of 5.79–579.3 mg/kg body weight, the extract caused hypoalbuminaemia, hypoglycaemia, hypercholesterolaemia, increased levels of alanine amino transferase (ALT) and alkaline phosphatase. Cabbage also caused necrosis of hepatocytes, glomerular and tubular necrosis, cylinduria and perivascular cuffing in the brain. At dose level of 5.79 mg/kg and above, cabbage may cause abortion during an early stage of pregnancy due to possible loss of endometrial gland. The combined extract of pumpkin and leek caused haematopoiesis, neutropenia, lymphocytosis, eosinophilia, hypoglycaemia, hypocholesterolaemia, elevation of ALT levels, necrosis of hepatocytes and brain tissues, kidney damage and suppression of the development of ovarian follicle.

Conclusions
Brassica oleracea var. capitata may cause anaemia, immunosuppression, hypoglycaemia, hypercholesterolaemia, abortion, liver and kidney damage. The combined extract of leek and pumpkin may cause haematopoeisis, immunostimulation, hypoglycaemia, hypocholesterolaemia, brain problem and infertility.
Clinical ladders
Authors: Boyd William , Witte Laura , Herman Lawrence 
Published: July 2023

ABSTRACT
As the need for more healthcare providers increases, so does the need for leaders in the healthcare setting. Physician associates/assistants (PAs) are primed to step into these positions, given their key role in the clinical setting. Clinical ladder models are an established system for clinical, administrative, and academic advancement for healthcare providers. Although more research is needed, clinical ladders provide the opportunity for career advancement while helping to promote professional development and improve retention. Moreover, the opportunity for advancement via clinical ladders may help to prevent clinician burnout and turnover by providing more opportunities and career directions.