bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advancements in hatchery production of red snapper Lutjanus campechanus: Exclusive use of small strain rotifers as initial prey for larval rearing
Authors: Buchalla Yole , McGuigan Charles James , Stieglitz John D. , Hoenig Ronald H. , Tudela Carlos E. , Darville Kimberly G. , Ibarra‐Castro Leonardo , Benetti Daniel 
Published: June 2023

AbstractThe red snapper (Lutjanus campechanus) has one of the most valued fisheries in the southeastern United States and has been largely studied for its aquaculture potential with varied results that revealed production challenges. This study focused on addressing challenges in larval rearing and juvenile production of this species. Broodstock fish were acclimated in a 60 m3 tank equipped with recirculating aquaculture system (RAS) and temperature control. After 1 year in captivity, fish volitionally spawned and larval rearing trials were conducted in replicated flow‐through seawater tanks ranging from 0.4 to 2.4 m3, at a temperature range of 24–26°C. Enriched s‐strain rotifers, Brachionus rotundiformis (lorica length of 100–210 μm), were used for first feeding. Enriched Artemia sp. were gradually introduced after 18 days post hatch (DPH). The survival rate before metamorphosis (12 DPH) averaged 66.09 ± 0.08%. Survival rate from yolk‐sac larvae to post‐metamorphic early juvenile averaged 4.43 ± 0.01%. At 40 DPH, early red snapper juveniles averaged 0.35 ± 0.02 g in weight and 28.83 ± 0.60 mm in standard length. A total of 31,849 fully weaned juveniles were harvested. This study demonstrates that small strain rotifers can be used exclusively as first feeding prey organism for red snapper larvae, representing a significant advancement for red snapper juvenile production.
Testing for adaptive changes linked to range expansion following a single introduction of the fall webworm
Authors: Dai Jin‐Xu , Cao Li‐Jun , Chen Jin‐Cui , Yang Fangyuan , Shen Xiu‐Jing , Ma Li‐Jun , Hoffmann Ary Anthony , Chen Min , Wei Shu‐Jun 
Published: June 2023

AbstractAdaptive evolution following colonization can affect the impact of invasive species. The fall webworm (FWW) invaded China 40 years ago through a single introduction event involving a severe bottleneck and subsequently diverged into two genetic groups. The well‐recorded invasion history of FWW, coupled with a clear pattern of genetic divergence, provides an opportunity to investigate whether there is any sign of adaptive evolution following the invasion. Based on genome‐wide SNPs, we identified genetically separated western and eastern groups of FWW and correlated spatial variation in SNPs with geographical and climatic factors. Geographical factors explained a similar proportion of the genetic variation across all populations compared with climatic factors. However, when the two population groups were analysed separately, environmental factors explained more variation than geographical factors. SNP outliers in populations of the western group had relatively stronger response to precipitation than temperature‐related variables. Functional annotation of SNP outliers identified genes associated with insect cuticle protein potentially related to desiccation adaptation in the western group and genes associated with lipase biosynthesis potentially related to temperature adaptation in the eastern group. Our study suggests that invasive species may maintain the evolutionary potential to adapt to heterogeneous environments despite a single invasion event. The molecular data suggest that quantitative trait comparisons across environments would be worthwhile.
Assessing spoken lexical and lexicogrammatical proficiency using features of word, bigram, and dependency bigram use
Authors: Kyle Kristopher , Eguchi Masaki 
Published: June 2023

AbstractThe measurement of second language (L2) productive lexical proficiency has driven a great deal of research over the past two decades. Research has indicated that more proficient speakers and writers tend to use a wider range of words and that more proficient writers tend to use words that are more sophisticated (less frequent in reference corpora). Research over the past 15 years has also demonstrated that the way words are used in context (i.e., collocation use) is also an important indicator of both written and spoken proficiency. In this study, we extend recent research that has modeled writing proficiency using collocation indices based on grammatical dependencies (e.g., verb–direct object) to spoken contexts. In particular, we model speaking proficiency scores from a large corpus of oral proficiency interview responses using a range of well‐known indices of productive proficiency and newly developed grammatical dependency indices. The results indicated that all index types demonstrated small to moderate correlations with speaking proficiency individually but explained a large proportion of the variance when used in a multivariate model that included dependency collocation indices.
Nausea is the only symptom associated with delayed gastric emptying in pediatric functional dyspepsia patients
Authors: De Keukelaere Marijke , Carbone Florencia , van Hoeve Karen , Tack Jan , Hoffman Ilse 
Published: June 2023

AbstractObjectivesFunctional dyspepsia (FD) is a heterogeneous functional gastrointestinal disorder (FGID) with a highly prevalent symptom complex. The aim of our study is to investigate the relation between symptoms of FD and results of gastric emptying (GE) breath test in children.MethodsThis study included patients (6–17 years old) presented at the general gastroenterology outpatient clinic with dyspeptic symptoms (Rome IV criteria) and underwent careful history taking with clinical examination. A GE breath test with a13C‐octanoic acid labeled (250 kcal) solid meal was performed and dyspepsia symptom scores, clarified using pictograms for postprandial fullness, bloating, belching, nausea, vomiting, epigastric pain and burning, were obtained every 15 min ranging from 0 to 4 for a total of 240 min. The severity of the complaints (overall and individual symptoms) as displayed by the symptom questionnaire was compared between normal and delayed GE groups. The relationship between GE time and the severity of FD symptoms was assessed using Mann–Whitney test.ResultsThirty nine FD patients (55% girls; mean age: 11.9 ± 3.3 years) participated in the study. Of these, 43% had delayed GE. The overall symptom severity in patients with delayed GE was similar to the symptoms of patients with a normal GE rate (149.5 ± 12.7 points vs. 123.9 ± 9.0;p = 0.19). Individual symptoms scores showed only nausea to be significantly increased in the group with delayed GE (21.5 ± 1.9 points vs. 33.2 ± 4.6;p = 0.048,p < 0.1).ConclusionEspecially in children with nausea as presenting symptom of FD, a low threshold should be withheld to perform a GE breath test.
Evolutionary lineage explains trait variation among 75 coexisting grass species
Authors: Donnelly Ryan C. , Wedel Emily R. , Taylor Jeffrey H. , Nippert Jesse B. , Helliker Brent R. , Riley William J. , Still Christopher J. , Griffith Daniel M. 
Published: June 2023

Summary

Evolutionary history plays a key role driving patterns of trait variation across plant species. For scaling and modeling purposes, grass species are typically organized into C3 vs C4 plant functional types (PFTs). Plant functional type groupings may obscure important functional differences among species. Rather, grouping grasses by evolutionary lineage may better represent grass functional diversity.

We measured 11 structural and physiological traits in situ from 75 grass species within the North American tallgrass prairie. We tested whether traits differed significantly among photosynthetic pathways or lineages (tribe) in annual and perennial grass species.

Critically, we found evidence that grass traits varied among lineages, including independent origins of C4 photosynthesis. Using a rigorous model selection approach, tribe was included in the top models for five of nine traits for perennial species. Tribes were separable in a multivariate and phylogenetically controlled analysis of traits, owing to coordination of important structural and ecophysiological characteristics.

Our findings suggest grouping grass species by photosynthetic pathway overlooks variation in several functional traits, particularly for C4 species. These results indicate that further assessment of lineage‐based differences at other sites and across other grass species distributions may improve representation of C4 species in trait comparison analyses and modeling investigations.
The high‐light‐induced protein SliP4 binds to NDH1 and photosystems facilitating cyclic electron transport and state transition in Synechocystis sp. PCC 6803
Authors: Alvarenga‐Lucius Luna , Linhartová Markéta , Schubert Hendrik , Maaß Sandra , Becher Dörte , Hess Wolfgang R. , Sobotka Roman , Hagemann Martin 
Published: June 2023

Summary

An increasing number of small proteins has been identified in the genomes of well‐annotated organisms, including the model cyanobacterium Synechocystis sp. PCC 6803. We describe a newly assigned protein comprising 37 amino acids that is encoded upstream of the superoxide dismutase SodB encoding gene.

To clarify the role of SliP4, we analyzed a Synechocystis sliP4 mutant and a strain containing a fully active, Flag‐tagged variant of SliP4 (SliP4.f).

The initial hypothesis that this small protein might be functionally related to SodB could not be supported. Instead, we provide evidence that it fulfills important functions related to the organization of photosynthetic complexes. Therefore, we named it a small light‐induced protein of 4 kDa, SliP4. This protein is strongly induced under high‐light conditions. The lack of SliP4 causes a light‐sensitive phenotype due to impaired cyclic electron flow and state transitions. Interestingly, SliP4.f was co‐isolated with NDH1 complex and both photosystems. The interaction between SliP4.f and all three types of complexes was further confirmed by additional pulldowns and 2D‐electrophoreses.

We propose that the dimeric SliP4 serves as a molecular glue promoting the aggregation of thylakoid complexes, which contributes to different electron transfer modes and energy dissipation under stress conditions.
Drought response strategies are coupled with leaf habit in 35 evergreen and deciduous oak (Quercus) species across a climatic gradient in the Americas
Authors: Kaproth Matthew A. , Fredericksen Brett W. , González‐Rodríguez Antonio , Hipp Andrew L. , Cavender‐Bares Jeannine 
Published: June 2023

Summary

Distinct survival strategies can result from trade‐offs in plant function under contrasting environments. Investment in drought resistance mechanisms can enhance survivorship but result in conservative growth. We tested the hypothesis that the widespread oaks (Quercus spp.) of the Americas exhibit an interspecific trade‐off between drought resistance and growth capacity.

Using experimental water treatments, we isolated adaptive trait associations among species in relation to their broad climates of origin and tested for correlated evolution between plant functional responses to water availability and habitat.

Across all lineages, oaks displayed plastic drought responses – typically acclimating through osmolyte accumulation in leaves and/or employing conservative growth. Oaks from xeric climates had higher osmolytes and reduced stomatal pore area index, which allows for moderated gas exchange and limits tissue loss.

Patterns suggest drought resistance strategies are convergent and under strong adaptive pressure. Leaf habit, however, mediates the growth and drought resistance strategies of oaks. Deciduous species, and evergreen species from xeric climates, have increased drought tolerance through osmoregulation, which allows for continuous, conservative growth. Evergreen mesic species show limited drought resistance but could enhance growth under well‐watered conditions. Consequently, evergreen species from mesic environments are especially vulnerable to chronic drought and climate change.
SlERF.H6 mediates the orchestration of ethylene and gibberellin signaling that suppresses bitter‐SGA biosynthesis in tomato
Authors: Hao Yingchen , Xiang Lijun , Lai Jun , Li Chun , Zhong Yue , Ye Weizhen , Yang Jie , Yang Jun , Wang Shouchuang 
Published: June 2023

Summary
Steroidal glycoalkaloids (SGAs) constitute a characteristic class of antinutritional metabolites that are found in certain Solanum species. Despite the considerable studies on SGA biosynthesis, the mechanisms of crosstalk between hormone signaling pathways that regulate SGA content still remain to be elucidated.
Here, we performed a metabolic genome‐wide association study (mGWAS) based on the levels of SGA metabolites and identified SlERF.H6 as a negative regulator of bitter‐SGA biosynthesis. SlERF.H6 repressed the expression of SGA biosynthetic glycoalkaloid metabolism (GAME) genes and caused a subsequent decrease in the abundance of bitter SGAs. Furthermore, SlERF.H6 were shown to act downstream of GAME9, a regulator of SGA biosynthesis in tomato.
We also uncovered the interplay between ethylene and gibberellin (GA) signaling in regulating SGA biosynthesis. SlERF.H6, acting as a downstream component in ethylene signaling, modulated GA content by inhibiting SlGA2ox12 expression. Increasing levels of endogenous GA12 and GA53 in SlERF.H6‐OE could inhibit of GA on SGA biosynthesis.
Additionally, 1‐aminocyclopropane‐1‐carboxylic acid (ACC) treatment decreased the stability of SlERF.H6, weakening its inhibition on GAME genes and SlGA2ox12, and caused bitter‐SGA accumulation. Our findings reveal a key role of SlERF.H6 in the regulation of SGA biosynthesis through the coordinated ethylene‐gibberellin signaling.
Analysis of real‐world visual ergonomics at the visual display unit
Authors: Kolbe Oliver , Müller Jennifer , Degle Stephan , Anders Christoph 
Published: June 2023

AbstractIntroductionIn this interventional study, the ergonomic workplace set‐up and the impact of character size on subjectively estimated working productivity and computer vision syndrome (CVS) were evaluated in the field.MethodsThe number of displays and their size, resolution, surface structure, position in the room and relation to the eye were evaluated for 152 units. CVS was assessed using the CVS‐Questionnaire. Habitually used character size for an uppercase E was recorded and compared to the ISO 9241–303:2011, national standards (e.g., ANSI/HFES 100–2007) and national guidelines (e.g., German DGUV Information 215–410). In case of failure to comply with these standards, character size was increased to 22 angular minutes to reach the recommended ranges. Reasons for returning to former or smaller character sizes were recorded, and subjectively perceived changes in productivity were estimated by the participants using a visual analogue scale before and 2 weeks after the intervention using a questionnaire.ResultsThe average visual display unit consisted of two non‐glare (matt) 24″ widescreen monitors that were located approximately 73 cm (primary) and 76 cm (secondary) from the eyes. The mean (SD) habitually set character size was 14.29 angular minutes (3.53) and therefore both statistically and clinically significantly too small compared with ISO 9241–303:2011 (p < 0.001). Increasing the character size to 22 angular minutes produced a 26% reduction in subjectively rated productivity (p < 0.001). No significant correlation between character size and symptoms of CVS was demonstrated.ConclusionsIn the workplaces investigated, recommendations for character size were not adhered to. This resulted in a reduction in productivity and was not compatible with some of the work requirements, for example, obtaining a broad overview of a spreadsheet.
Feasibility and safety of introducing cashew nut spread in infant diets—A randomized trial
Authors: Palmer Debra J. , Silva Desiree T. , Prescott Susan L. 
Published: June 2023

AbstractBackgroundTo reduce peanut allergy prevalence, infant feeding guidelines now recommend introducing peanuts in an age‐appropriate form (such as peanut butter) as part of complementary feeding. However, due to a lack of randomized trial evidence, most infant feeding and food allergy prevention guidelines do not include tree nuts. The aims of this trial were to determine safety and feasibility of dosage consumption recommendations for infant cashew nut spread introduction.MethodsThis is a parallel, three‐arm (1:1:1 allocation), single‐blinded (outcome assessors), randomized controlled trial. General population term infants were randomized at 6–8 months of age to either a one teaspoon (Intervention 1 n = 59) or increasing dosage regime of one teaspoon at 6–7 months, two teaspoons at 8–9 months, and three teaspoons from 10 months of age onwards (Intervention 2 n = 67) cashew nut spread, both three times per week, or no specific advice on cashew introduction (Control n = 70). At 1 year of age, food challenge proven IgE‐mediated cashew nut allergy was assessed.ResultsCompliance in Intervention 1 (92%) was higher than Intervention 2 (79%), p = .04. Only one infant had delayed (at 5 h) facial swelling and eczema flare to cashew introduction at 6.5 months, but no cashew allergy at 1 year. Only one infant (Control) had cashew allergy at 1 year, and this infant had not been introduced to cashew prior to 12 months of age.ConclusionRegular infant consumption of one teaspoon of cashew nut spread three times per week from 6 to 8 months of age was found to be feasible and safe.
Outcomes in pediatric liver transplant recipients receiving bilateral continuous erector spinae plane blocks
Authors: Dewey Megan M. , Kodali Ahalya , Jiao York , Drobish Julie K. 
Published: June 2023

AbstractBackgroundRegional anesthesia allows for opioid‐sparing and enhanced recovery after many major surgeries. Erector spinae blockade, with reduced bleeding risk and the option for continuous infusion, offers an opportunity to promote this principle in pediatric liver transplant patients. Our goal was to evaluate pain scores, opioid use, and return of bowel function following continuous ESP blockade in pediatric liver transplant recipients.MethodsThis retrospective cohort study included extubated patients who received a liver transplant at St. Louis Children's Hospital from July 2016 to July 2021. The control group, which did not meet the criteria for ESP blockade and received standard analgesia regimens, was compared to the group receiving continuous ESP blockade. Measured outcomes included pain scores, opioid consumption through postoperative day two, date of first bowel movement, and length of stay in the ICU and the hospital.ResultsPatient demographics between control and ESP groups showed no significant differences. Pain scores between control and ESP groups also showed no significant differences. Intraoperative and postoperative opioid requirements, studied in oral morphine equivalents per kilogram (OME/kg), were significantly lower for patients with ESP blockade. Time to first bowel movement was also significantly earlier for the ESP group. No significant differences were found in length of ICU or hospital stay. There were no safety concerns or complications related to ESP blockade.ConclusionsUse of continuous ESP blockade resulted in reduced opioid consumption through postoperative day two and earlier return of bowel function.
The act‐type theory of propositions as a theory of what is said
Authors: Hodgson Thomas 
Published: June 2023

AbstractI propose a version of the act‐type theory of propositions, following Hanks and Soames. According to the theory, propositions are types of act of predication. The content of a sentence is the type of such act performed when that sentence is uttered. A consequence of this theory is that the structure of the content of a sentence will mirror the structure of that sentence. I defend this consequence of the theory from two important objections. I then argue that this theory is well motivated because it can be part of a theory of what is said.
Her strength: Reflections on nurse home‐visiting
Authors: Campbell Karen A. 
Published: June 2023

AbstractThe Nurse‐Family Partnership is a targeted parenting intervention designed to improve maternal and child health. In Canada it is delivered exclusively by public health nurses who provide complex care to adolescent girls and young women. A process evaluation was conducted to better understand the experiences of public health nurses delivering the Nurse‐Family Partnership program in Canada. Although the traditional qualitative data analysis led to the creation of significant findings and clinical implications, it lacked the “heart” of public health nursing practice. Through a reflective process, and to present an evocative account of the multifaceted nursing care provided by the study participants, direct quotes were used to create a poetic display of nurses’ experiences. Through the power of found poetry, the complexities of clients’ lives, as well as the challenges and joys of home‐visitation nursing, were illuminated.
A semiparametric Bayesian model for queueing arrival processes: An application to call centers
Authors: Kuzu Kaan , Soyer Refik , Tarimcilar Murat T. 
Published: June 2023

Nonhomogeneous Poisson process models have commonly been used to analyze and forecast arrivals. Such processes require specification of intensity (arrival rate) functions, which are typically defined in a parametric form. The accuracy of the parametric models is highly sensitive to the choice of the specific intensity function for the arrival process. We use a Bayesian framework by proposing a nonparametric form for the intensity function and introduce a robust semiparametric model. The model is suitable for analyzing both time of arrival data and interval censored count data and can capture both monotonic and non‐monotonic arrival intensity. The intensity function in the model can be modulated to incorporate auxiliary information as well as seasonal and random effect components. We develop the Bayesian analysis of the proposed model and implement it on two real call center datasets with different characteristics. We also consider several extensions to our model and develop their Bayesian analyses. Our random effects extension model with cumulative baseline intensity changing on the days of the week and interday correlation with the Markov evolution extension model both provide high predictive accuracy. We also show that our proposed semiparametric model has robust performance for out‐of‐sample predictions. Accurate predictions of arrivals will assist managers in determining appropriate staffing levels and effective workforce scheduling, resulting in more efficient operations.