bluuebunny/crossref_metadata_2025_split huggingface dataset:
Healing potentiality of blood clot, S-PRF and A-PRF as scaffold in treatment of non-vital mature single rooted teeth with chronic peri-apical periodontitis following regenerative endodontic therapy: randomized clinical trial
Authors: Darwish Omnia Badawy , Aziz Said Mohamed Abdel , Sadek Hany Samy 
Published: January 2025

Abstract
Objectives
This randomized prospective controlled trial investigated the effectiveness of different strategies of regenerative endodontic therapy on necrotic mature anterior teeth with chronic periapical periodontitis with 18 months follow up.

Methods
A total analyzed 51 adult participant with mature single rooted teeth having necrotic pulp with chronic periapical periodontitis (PAI ≥ 3) were selected. Patients had been randomly categorized into three distinct groups (n = 17 each group). All groups received the same treatment on the first visit. After 2 weeks, regenerative treatment was performed using either blood clot technique, Standard-PRF and Advanced-PRF approach. A standardized radiograph was taken, and the patients instructed for 6, 12 and 18 months follow up periods. Fisher's exact test was applied to compare the frequency of PAI scores at different follow-up intervals between the three groups.

Results
The results showed radiographic success at 18 months (58.8% in blood group, 94.1% in S-PRF group and 76.5% in A-PRF group). There was no statistically significant difference between the three groups according to incidence of healing at 6, 12 and 18 months. Clinical success was 82.4% in blood group and 88.2% in both S-PRF and A-PRF groups. There was no significant difference between the three groups (p = 1). The overall success (clinical and radigraphic) was 76.5%. Incidence of the gaining sensitivity after 12 and 18 months was 29.4% with A-PRF group and 41.2% within the S-PRF group, 17.6% with BC group.

Conclusion
PRF based regenerative technique may outperform the blood clot technique in treatment of non-vital mature teeth with chronic periapical periodontitis. There is a need for future randomized clinical studies to consolidate procedures in this field with more prolonged evaluation periods.

Clinical trial registration
The study was retrospectively registered with ClinicalTrials.gov (ID: NCT04606719) in 28/10/2020.
The influence of sintering of osteoporotic vertebral fractures on the sagittal lumbar profile and degenerative changes
Authors: Beyersdorf Christoph , Prost Max , Röckner Melanie Elisabeth , Maus Uwe Martin , Jacobs Cornelius , Scheyerer Max Joseph 
Published: January 2025

Abstract
Background
Osteoporosis, a skeletal disorder affecting nearly 20% of the global population, poses a significant health concern, with osteoporotic vertebral body fractures (VBF) representing a common clinical manifestation. The impact of osteoporotic sintering fractures in the thoracolumbar spine on the sagittal lumbar profile is incompletely understood and may lead to the onset of clinical symptoms in previously asymptomatic patients.

Methods
This retrospective single-center study analyzed data from patients presenting with osteoporotic spine fractures between 2017 and 2022. Patient selection involved stringent inclusion and exclusion criteria, focusing on radiologically documented osteoporotic sintering fractures in the thoracolumbar junction (TH11-L2). Clinical parameters were recorded and analyzed, alongside lateral-view radiographic assessments utilizing the IDS 7-PACS®-System (Sectra, Linköping, Sweden). Measurements included total lumbar lordosis, lordosis caudal to the fracture, kyphosis of the fractured vertebra, and sacral slope. Statistical analysis was conducted using SPSS 27 (IBM, Armonk, USA).

Results
Thirty patients (73.3% female, 26.7% male) met the inclusion criteria, with an average age of 82.4 years. Analysis revealed a significant increase in kyphosis of the fractured vertebra in the thoracolumbar spine (p < 0.0001) following further sintering of osteoporotic VBF with increased lordosis caudal to the fracture (p < 0.0001). Total lumbar lordosis remained unchanged, alongside sacral slope measurements (p = 0.612 and p = 0.863, respectively).

Conclusion
Progressive sintering of osteoporotic fractures in the thoracolumbar junction accentuates lordosis in underlying segments, potentially exacerbating degenerative changes and symptomatic manifestations. Thus, prioritizing interventions aimed at preventing progressive sintering and restoring sagittal balance is paramount in optimizing treatment outcomes for affected individuals.
Quality of life in people with syndromic heritable thoracic aortic disease and their relatives: a qualitative interview based study
Authors: Velvin Gry , Johansen Heidi , Aune Gunnbjørg , Fugl-Meyer Kerstin , Geirdal Amy Østertun 
Published: January 2025

Abstract
Introduction
The purpose of this study was to investigate perceptions and opinions on what constitutes determinants for quality of life (QoL) in individuals with syndromic Heritable Aortic Disease (sHTAD), utilizing a qualitative study approach. Further to discuss clinical implications and direction for research.

Method
A qualitative focus group interview study was conducted of 47 adults (Marfan syndrome (MFS) = 14, Loeys-Dietz syndrome (LDS) = 11, vascular Ehlers Danlos syndrome (EDS) = 11, relatives = 11). The interviews were digitally recorded and transcribed verbatim. Significant themes were identified, extracted, and organised undergoing content analyses.

Results
The two main themes and 10 subthemes identified; I. Psychosocial well-being; (i) Social engagement and activity, (ii) Self-sufficient in daily living, (iii) Participation in education and work life, (iv) Coping with fear related to the disease, (v) Being able to control and accept fatigue and pain, (vi) Maintaining active engagement with family and friends (vii) Finding health-promoting physical activities. II. Monitoring and meetings with the health service: (viii) Feeling safe and receiving coordinated care, (ix) Being recognized, seen, and accepted, (x) Receiving factual and sober information and advice. The sub-themes seemed mutually interrelated in terms of barriers, strategies, and facilitators for improving quality of life. There was high degree of consensus regarding the factors emphasized as important for QoL among the various diagnostic groups and the relatives.

Conclusion
Based on our findings, to improve QoL in patients with sHTAD we should more effectively integrate the patient`s perspectives and voice on the elements crucial to QoL. In addition, it is vital for developing and customizing validated questionnaires to accurately reflect the factors deemed significant by this specific patient cohort. The research is limited on patients’ perspectives on QoL, and more research is warranted. This might also be crucial for identifying relevant validated QoL instruments that reflect the patients` perceptions of what is vital for QoL.
Phenotypic diversity of CTCs and tdEVs in liquid biopsies of tumour-draining veins is linked to poor prognosis in colorectal cancer
Authors: Cieslik Stefan A. , Zafra Andrés G. , Driemel Christiane , Sudarsanam Monica , Cieslik Jan-Philipp , Flügen Georg , Dizdar Levent , Krieg Andreas , Vaghiri Sascha , Ashmawy Hany , Fung Stephen , Wilms Miriam , Terstappen Leon W. M. M. , Nanou Afroditi , Neubauer Hans , Rahbari Nuh N. , Knoefel Wolfram T. , Stoecklein Nikolas H. , Neves Rui P. L. 
Published: January 2025

Abstract
Background
Circulating tumour cells (CTCs) and tumour-derived extracellular vesicles (tdEVs) have great potential for monitoring therapy response and early detection of tumour relapse, facilitating personalized adjuvant therapeutic strategies. However, their low abundance in peripheral blood limits their informative value. In this study, we explored the presence of CTCs and tdEVs collected intraoperatively from a tumour-draining vein (DV) and via a central venous catheter (CVC) prior to tumour resection.

Methods
CellSearch analyses of 395 blood samples from 306 patients with gastrointestinal tumours and 93 blood samples from healthy donors were used to establish and validate gates for the automated detection of CTCs and tdEVs with ACCEPT software and R scripts. The selected gate settings were applied to 227 samples of 142 patients with colorectal cancer (CRC) from two independent collectives. Phenotypic features were obtained via numeric analysis of their fluorescence signals (e.g. size, shape, and intensity) and were used for calculating diversity using Shannon index (SI) of clusters generated via the k-means algorithm after Uniform Manifold Approximation and Projection (UMAP) pre-processing, and standard deviation (SD).

Results
CTCs and tdEVs were more abundant in the DV samples compared to CVC samples (p < 0.05). tdEVs were detected in higher numbers than CTCs in both compartments. Importantly, tdEVs in CVCs were associated with tumor spread, whereas CTCs in DVs were linked to tumor size. In both compartments, the prognostic value of tdEVs for overall survival (OS) surpassed that of CTCs, as demonstrated by univariate, multivariate, and Kaplan-Meier analyses. CTCs and tdEVs in DVs were phenotypically distinct, being larger, more eccentric, and displaying stronger cytokeratin intensities (p < 0.05) compared to those in CVC samples. Furthermore, increased diversity in CTC and tdEV phenotypes was significantly associated with shorter survival, validating the prognostic relevance of the SD-diversity metric.

Conclusion
Our study demonstrates that DV sampling significantly enhances the detection of prognostically relevant CTCs and tdEVs in CRC patients, underscoring the superior prognostic significance of tdEVs compared to CTCs. Importantly, the combined phenotypic diversity of both markers emerges as a more powerful biomarker than their enumeration alone. These findings suggest that comprehensive, automated analysis of CTCs and tdEVs in DVs may open new avenues for tailoring individualized therapies in CRC patients.
Batch and semi-continuous fermentation with Parageobacillus thermoglucosidasius DSM 6285 for H2 production
Authors: Ardila Magda S. , Aliyu Habibu , de Maayer Pieter , Neumann Anke 
Published: January 2025

Abstract
Background
Parageobacillus thermoglucosidasius is a facultatively anaerobic thermophile that is able to produce hydrogen (H2) gas from the oxidation of carbon monoxide through the water–gas shift reaction when grown under anaerobic conditions. The water–gas shift (WGS) reaction is driven by a carbon monoxide dehydrogenase–hydrogenase enzyme complex. Previous experiments exploring hydrogenogenesis with P. thermoglucosidasius have relied on batch fermentations comprising defined media compositions and gas atmospheres. This study evaluated the effects of a semi-continuous feeding strategy on hydrogenogenesis.

Results
A batch and two semi-continuous fermentations, with feeding of the latter fresh media (with glucose) in either 24 h or 48 h intervals were undertaken and H2 production, carbon monoxide dehydrogenase (CODH) activity, and metabolite consumption/production were monitored throughout. Maximum H2 production rates (HPR) of 0.14 and 0.3 mmol min−1, were observed for the batch and the semi-continuous fermentations, respectively. Daily feeding attained stable H2 production for 7 days, while feeding every 48 h resulted in high variations in H2 production. CODH enzyme activity correlated with H2 production, with a maximum of 1651 U mL−1 on day 14 with the 48 h feeding strategy, while CODH activity remained relatively constant throughout the fermentation process with the 24 h feeding strategy.

Conclusions
The results emphasize the significance of a semi-continuous glucose-containing feed for attaining stable hydrogen production with P. thermoglucosidasius. The semi-continuous fermentations achieved a 46% higher HPR than the batch fermentation. The higher HPRs achieved with both semi-continuous fermentations imply that this approach could enhance the biohydrogen platform. However, optimizing the feeding interval is pivotal to ensuring stable hydrogen production.
Seismic and GNSS strain-based probabilistic seismic hazard evaluation for northern Chile using DAS Magnitude Scale
Authors: Das Ranjit , Meneses Claudio , Wang Hua 
Published: January 2025

Abstract
Background
Probabilistic Seismic Hazard Assessment (PSHA) is a leading methodology for determining key ground motion parameters such as Peak Ground Acceleration (PGA) and spectral acceleration (SA), essential for structural design. This approach uses extensive earthquake data, typically spanning over a century, leveraging frequency and magnitude statistics. However, long-term ground shaking probabilities may not always be accurately captured by traditional data-driven methods. To address these limitations, this study develops a PSHA map for Northern Chile using both seismic and GNSS (Global Navigation Satellite System) data. A curated homogeneous earthquake catalog, based on the advanced seismic moment magnitude scale Mwg(Das Magnitude Scale), replaces the traditional Mw scale to ensure superior accuracy, particularly for intermediate and smaller earthquakes.

Results
Using the earthquake catalog, seismicity parameters ‘a’ and ‘b’ from the Gutenberg-Richter relationship were derived. Seismogenic modeling and Ground Motion Models (GMMs) were applied to estimate ground motion probabilities for a 475-year return period. Additionally, a PSHA map was constructed using GNSS strain rates, translating velocity-derived strain rates into seismic moment rates and ground shaking probabilities for seismic source zones. Comparative analyses revealed higher PGA values from GNSS strain data compared to seismic catalog data. GNSS strain data proved invaluable for refining seismic segmentation in Northern Chile, enhancing the precision of PSHA calculations.

Conclusions
A PSHA map for Northern Chile, synthesizing seismic catalog data and GNSS strain rates using a Logic Tree-based algorithm, has been developed for a 475-year return period. This map provides a critical tool for generating seismic hazard assessments aligned with building codes and emergency planning protocols. By integrating GNSS strain rates and seismic data, this study advances the reliability and accuracy of long-term ground shaking predictions.
Causal estimation of FTX collapse on cryptocurrency: a counterfactual prediction analysis
Authors: Khan Khalid , Khurshid Adnan , Cifuentes-Faura Javier 
Published: January 2025

AbstractThis study uses the Bayesian structural model to assess the causal effect of the futures exchange (FTX) insolvency on cryptocurrencies from October 2022 to December 14, 2022. Findings show that FTX insolvency negatively impacts cryptocurrencies. Moreover, the results indicate rapid divergence from counterfactual predictions, and the actual cryptocurrencies are consistently lower than would have been expected in the absence of the FTX collapse. Cryptocurrency is reacting strongly to the uncertainty caused by insolvency. In relative terms, the collapse of FTX has been highly detrimental to Solana and Ethereum. Furthermore, the outcomes show that cryptocurrencies would not have been negatively affected if the intervention had not occurred. FTX collapsed owing to a mismatch between the assets and liabilities. The industry is still mostly unregulated, and regulators must act quickly, highlighting the need for outstanding innovation and decentralized and trustless technology adoption.
Mir-615-5p inhibits cervical cancer progression by targeting TMIGD2
Authors: Zhao Yan , Chen Haitao , Zhang Wenhui , Shang Wei , Cao Jinwei , Zhao Huijuan , Zou Zhixiang 
Published: January 2025

Abstract
Background
Cervical cancer (CC) is a prevalent gynecological malignancy, contributing to a substantial number of fatalities among women. MicroRNAs (miRNAs) have emerged as promising biomarkers with significant potential for the early detection and prognosis of CC.

Objective
This study aimed to explore the clinical significance and biological role of miR-615-5p in CC, with the goal of identifying novel biomarkers for this disease.

Materials and methods
The levels of miR-615-5p and TMIGD2 mRNA in tissue samples and cells were quantified through quantitative reverse transcription real-time PCR, followed by statistical analyses to investigate the correlation between miR-615-5p and clinical data. The effects of miR-615-5p on the proliferation and metastasis of CC cells were evaluated using the Cell Counting Kit-8 and Transwell assays. The potential mechanism of miR-615-5p was elucidated by bioinformatics analyses and Dual-luciferase reporter assay. Western blotting was employed to measure the protein levels of TMIGD2.

Results
In CC, the downregulation of miR-615-5p was related to poor prognosis and emerged as an independent prognostic factor. The levels of miR-615-5p were reduced in CC cells. miR-615-5p overexpression restrained the proliferation and metastasis of CC cells. Furthermore, TMIGD2 was identified as a target gene regulated by miR-615-5p, and its expression was notably elevated in CC. The influence of miR-615-5p on the biological behaviors of CC cells was mediated through the modulation of TMIGD2.

Conclusions
Downregulation of miR-615-5p was a prognostic indicator of poor prognosis in CC. miR-615-5p exerted its tumor-suppressive effects by inhibiting cell growth and metastasis through the regulation of TMIGD2.
Exploring the effects of elaborated and motivational feedback on learning engagement in online scripted role discussion
Authors: Feng Qinna , Li Wenhao , Zhu Xueping , Li Xiuhan 
Published: January 2025

AbstractGiving feedback is key to online collaborative discussion, but the impact of feedback on role-scripts in online discussion remains unclear. To address this research gap, a mixed factorial experiment was conducted to investigate the potential effects and interactions of feedback type (elaborated vs. motivational feedback) and scripted roles (yes vs. no) on behavioral, cognitive, and emotional engagement. Fifty participants enrolled in an undergraduate blended course were randomly assigned to a role-script group (n = 25) and a role-free group (n = 25). Both groups received elaborated and motivational feedback sequentially. Mixed ANOVA results showed that (a) scripted roles significantly facilitated behavioral engagement; (b) elaborated feedback intrigued more cognitive engagement compared to motivational feedback; (c) the role-scripting strategy and feedback type had an interaction effect on behavioral engagement; and (d) there was a significant difference in cognitive engagement among various roles. The epistemic network analysis results revealed evolving cognitive engagement patterns associated with distinct roles when exposed to two distinct types of feedback. The implications of these findings for the design and implementation of online discussions are discussed.
Patient-reported outcome measures to assess mental and physical health status, functionality, and quality of life in patients with major depression or schizophrenia
Authors: San Luis , Arranz Belen , Romans Carlota , García Berta , Coromina Marta , Ortiz Sonia , Vilaplana Miriam , Soto Víctor , Villaescusa Ruth , Alvaros Joan 
Published: January 2025

Abstract
Background
Patient-reported outcomes measures (PROMs) are standardized self-administered tools that assess the patient’s opinion on the level of health, quality of life, and disability among other aspects. The objective of this study was to gather information on physical and mental health in patients with major mental illness using PROMs.

Methods
This was an observational, naturalistic, prospective study carried out in adult stabilized outpatients attended at nine Adult Mental Health Centers in Barcelona, Spain. All participants had a confirmed diagnosis of major depression disorder or schizophrenia (DSM-5) and were currently on drug treatment. Participants (n = 508) self-completed a baseline questionnaire for clinical data and PROMs scales (PHQ-19, SF-12, and WHODAS 2.0) at baseline and 9 months thereafter (n = 482).

Results
Mean (SD) age was 50.9 (13.2) years, and 83% of patients lived with their families. Although 93.9% of patients recognized having a mental illness, 15.7% did not know their diagnosis. When asked if they considered that during the last year their treatment had offered some type of improvement, 83.9% answered affirmatively. Patients reported that their degree of adherence to treatment was high (77%) and most of them (80%) believed the medication had a beneficial effect. Depressed patients showed both at baseline and at follow up significantly more depressive symptoms than the group with schizophrenia. In the schizophrenia group, a statistically significant improvement in depressive symptoms was noted at 9 months follow-up. We did not find significant differences within or between groups in quality of life (SF-12 scores) obtained at baseline and after 9 months of follow-up Both at baseline and at follow-up, patients with depression reported a significantly higher degree of disability (WHODAS scores) than those with schizophrenia.

Conclusions
PROMs can be used in real-world conditions to assess severity of disease, quality of life, and disability in major depression and schizophrenia. The present results are relevant for both patients and clinicians.
Validation of Härtel Surface Anatomical Landmarks for Locating the Foramen Ovale: A Computed Tomography Scan Analysis and Revised Technique Description
Authors: Saavedra Azcona Tomás , Villaescusa Miguel , Casto Florencia , Paolinelli Pablo , Dover Sophia E. , Plou Pedro L. , Ciraolo Carlos A. , Ajler Pablo M. 
Published: January 2025

BACKGROUND AND OBJECTIVES:
Härtel triangle provides surface landmarks for locating the foramen ovale (FO) when performing trigeminal nerve percutaneous procedures. Although widely adopted in clinical practice, there is no report that these landmarks have ever been formally validated through modern imaging techniques. Here we aim to validate Härtel anatomical landmarks using computed tomography scans and propose technical considerations for percutaneous trigeminal procedures.

METHODS:
Retrospective analysis of 198 FO from 99 adult head computed tomography scans. Measurements included distances from FO to external auditory canal (EAC), FO to midline, eye's midpupillary (MP) line to midline, and eye's inner canthus (IC) line to midline. Statistical analysis was performed, and results were compared with Härtel description.

RESULTS:
The mean distance from EAC to FO was 23.26 mm (SD: 3.00 mm). Distance from midline to FO was 25.43 mm overall (SD: 1.87 mm). Distance from midline to MP line was measured at 31.96 mm (SD: 1.89 mm). The mean distance from midline to IC line was 14.68 mm (SD: 1.73 mm).

CONCLUSION:
Härtel landmarks can be adjusted for greater accuracy when performing percutaneous trigeminal nerve procedures. The FO is located closer to the EAC and more medially situated than previously assumed. Revised technique suggests aiming the needle trajectory approximately 2 to 2.5 cm anterior to the tragus and targeting a point between the IC and MP lines rather than directly along the MP line. Excessive medial and posterior needle displacement should be avoided to prevent inadvertent vascular injury. These adjustments could enhance procedural accuracy and safety, improving patient outcomes.