bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimation of groundwater storage loss for the Indian Ganga Basin using multiple lines of evidence
Authors: Janardhanan Sreekanth , Nair Akhilesh S. , Indu J. , Pagendam Dan , Kaushika G. S. 
Published: January 2023

AbstractWe used remote sensing data, field observations and numerical groundwater modelling to investigate long-term groundwater storage losses in the regional aquifer of the Ganga Basin in India. This comprised trend analysis for groundwater level observations from 2851 monitoring bores, groundwater storage anomaly estimation using GRACE and Global Land Data Assimilation System (GLDAS) data sets and numerical modelling of long-term groundwater storage changes underpinned by over 50,000 groundwater level observations and uncertainty analysis. Three analyses based on different methods consistently informed that groundwater storage in the aquifer is declining at a significant rate. Groundwater level trend indicated storage loss in the range − 1.1 to − 3.3 cm year−1 (median − 2.6 cm year−1) while the modelling and GRACE storage anomaly methods indicated the storage loss in the range of − 2.1 to − 4.5 cm year−1 (median − 3.2 cm year−1) and − 1.0 to − 4.2 cm year−1 (median − 1.7 cm year−1) respectively. Probabilistic modelling analysis also indicated that the average groundwater storage is declining in all the major basin states, the highest declining trend being in the western states of Rajasthan, Haryana and Delhi. While smaller compared to the western states, average groundwater storage in states further towards east—Uttar Pradesh, Bihar and West Bengal within the basin are also declining. Time series of storage anomalies obtained from the three methods showed similar trends. Probabilistic storage analysis using the numerical model vetted by observed trend analysis and GRACE data provides the opportunity for predictive analysis of storage changes for future climate and other scenarios.
Digital twin-based multi-level task rescheduling for robotic assembly line
Authors: Yao Bitao , Xu Wenjun , Shen Tong , Ye Xun , Tian Sisi 
Published: January 2023

AbstractAssembly is a critical step in the manufacturing process. Robotic assembly technology in automatic production lines has greatly improved the production efficiency. However, in assembly process, dynamic disturbances such as processing time change and advance delivery may occur, which cause the scheduling deviation. Traditional scheduling methods are not sufficient to meet the real-time and adaptive requirements in smart manufacturing. Digital twin (DT) has the characteristics of virtual-reality interaction and real-time mapping. In this paper, we propose a DT-based framework of task rescheduling for robotic assembly line (RAL) and its key methodologies, thus to realize the timely and dynamic adjustment of scheduling plan under uncertain interferences. First, a DT model of RAL task rescheduling composed of physical entity (PE), virtual entity (VE), and virtual-reality interaction mechanism is proposed. Then, a mathematical model is established. By analyzing the adaptive objective thresholds from the perspectives of event trigger and user demand trigger, a DT-driven multi-level (production unit level and line level) rescheduling strategy is proposed. Taking both the computing time and solution quality into consideration, the precedence graph is introduced to propose a rescheduling approach based on an improved discrete fireworks algorithm. Finally, the effectiveness of the proposed model and approach are verified by task scheduling experiments of RAL.
RNA disruption is a widespread phenomenon associated with stress-induced cell death in tumour cells
Authors: Butler Phillipe , Pascheto Isabella , Lizzi Michayla , St-Onge Renée , Lanner Carita , Guo Baoqing , Masilamani Twinkle , Pritzker Laura B. , Kovala A. Thomas , Parissenti Amadeo M. 
Published: January 2023

AbstractWe have previously shown that neoadjuvant chemotherapy can induce the degradation of tumour ribosomal RNA (rRNA) in patients with advanced breast cancer, a phenomenon we termed “RNA disruption”. Extensive tumour RNA disruption during chemotherapy was associated with a post-treatment pathological complete response and improved disease-free survival. The RNA disruption assay (RDA), which quantifies this phenomenon, is now being evaluated for its clinical utility in a large multinational clinical trial. However, it remains unclear if RNA disruption (i) is manifested across many tumour and non-tumour cell types, (ii) can occur in response to cell stress, and (iii) is associated with tumour cell death. In this study, we show that RNA disruption is induced by several mechanistically distinct chemotherapy agents and report that this phenomenon is observed in response to oxidative stress, endoplasmic reticulum (ER) stress, protein translation inhibition and nutrient/growth factor limitation. We further show that RNA disruption is dose- and time-dependent, and occurs in both tumourigenic and non-tumourigenic cell types. Northern blotting experiments suggest that the rRNA fragments generated during RNA disruption stem (at least in part) from the 28S rRNA. Moreover, we demonstrate that RNA disruption is reproducibly associated with three robust biomarkers of cell death: strongly reduced cell numbers, lost cell replicative capacity, and the generation of cells with a subG1 DNA content. Thus, our findings indicate that RNA disruption is a widespread phenomenon exhibited in mammalian cells under stress, and that high RNA disruption is associated with the onset of cell death.
Androgen receptor-dependent regulation of metabolism in high grade bladder cancer cells
Authors: Katleba Kimberley D. , Tsamouri Maria-Malvina , Jathal Maitreyee , Baek Han Bit , Armenta Rebecca B. , Tepper Clifford G. , Cortopassi Gino , Ghosh Paramita M. , Mudryj Maria 
Published: January 2023

AbstractThe observed sex disparity in bladder cancer (BlCa) argues that androgen receptor (AR) signaling has a role in these malignancies. BlCas express full-length AR (FL-AR), constitutively active AR splice variants, including AR-v19, or both, and their depletion limits BlCa viability. However, the mechanistic basis of AR-dependence is unknown. Here, we depleted FL-AR, AR-v19, or all AR forms (T-AR), and performed RNA-seq studies to uncover that different AR forms govern distinct but partially overlapping transcriptional programs. Overlapping alterations include a decrease in mTOR and an increase of hypoxia regulated transcripts accompanied by a decline in oxygen consumption rate (OCR). Queries of BlCa databases revealed a significant negative correlation between AR expression and multiple hypoxia-associated transcripts arguing that this regulatory mechanism is a feature of high-grade malignancies. Our analysis of a 1600-compound library identified niclosamide as a strong ATPase inhibitor that reduces OCR in BlCa cells, decreased cell viability and induced apoptosis in a dose and time dependent manner. These results suggest that BlCa cells hijack AR signaling to enhance metabolic activity, promoting cell proliferation and survival; hence targeting this AR downstream vulnerability presents an attractive strategy to limit BlCa.
Effect of anthocyanins on gut health markers, Firmicutes-Bacteroidetes ratio and short-chain fatty acids: a systematic review via meta-analysis
Authors: Kapoor Payal , Tiwari Apoorv , Sharma Saloni , Tiwari Vandita , Sheoran Bhawna , Ali Usman , Garg Monika 
Published: January 2023

AbstractResearchers discovered that diets rich in anthocyanin-rich fruits and vegetables significantly impacted gut flora. To conclude, large-scale randomized controlled clinical trials are challenging to conduct; therefore, merging data from multiple small studies may aid. A systematic review collects and analyses all research on a particular subject and design. This comprehensive review and meta-analysis examined the influence of dietary anthocyanins on Firmicutes/Bacteroide (Fir/Bac) and short-chain fatty acids (SCFAs) content. The current meta-analysis followed the guidelines of PRISMA—the preferred reporting items for systematic reviews and meta-analyses. Diets high in anthocyanins substantially reduced the Fir/Bac ratio in the assessed trials. Among three SCFAs, the highest impact was observed on acetic acid, followed by propionic acid, and then butanoic acid. The meta-analysis results also obtained sufficient heterogeneity, as indicated by I2 values. There is strong evidence that anthocyanin supplementation improves rodent gut health biomarkers (Fir/Bac and SCFAs), reducing obesity-induced gut dysbiosis, as revealed in this systematic review/meta-analysis. Anthocyanin intervention duration and dosage significantly influenced the Fir/Bac ratio and SCFA. Anthocyanin-rich diets were more effective when consumed over an extended period and at a high dosage.
Sociodemographic predictors of flourishing among older adults in rural and urban Mongolia
Authors: Otgon Saranchuluun , Myagmarjav Sugarmaa , Burnette Denise , Lkhagvasuren Khorolsuren , Casati Fabio 
Published: January 2023

AbstractFlourishing is an eudaimonic dimension of psychological well-being associated with positive social and health outcomes. Determining correlates of health and well-being is critical for the development of evidence-based best practices, policies, and action plans that target older adults, especially in low- and middle-income countries where research on ageing, health and well-being is still scarce. The study aimed to determine the level of flourishing among older adults in Mongolia and to explore demographic and social factors that contribute to their flourishing in urban and rural areas of Mongolia. We used proportional quota sampling to select a non-probability sample of 304 community-dwelling older adults that reflected the national distribution of older age groups and rural/urban residency. We adapted and administered a widely used standardized questionnaire on flourishing and used multiple regression to establish correlates of flourishing. Study participants reported “very high” levels of flourishing; differences in median scores 53 for urban and 50 for rural older adults were significant. Sex ($$\beta =-\,2.09$$

β
=
-

2.09

, $$p=0.080$$

p
=
0.080

), level of education($$\beta =0.98$$

β
=
0.98

, $$p=0.009$$

p
=
0.009

) and receive help with ADLs ($$\beta =-\,2.29$$

β
=
-

2.29

, $$p=0.013$$

p
=
0.013

) were associated with flourishing in rural areas, as were self-rated health ($$\beta =1.14$$

β
=
1.14

, $$p=0.028$$

p
=
0.028

), number of social activities ($$\beta =0.050$$

β
=
0.050

, $$p=0.019$$

p
=
0.019

),and friends network ($$\beta =0.22$$

β
=
0.22

, $$p=0.035$$

p
=
0.035

) in urban areas. Despite facing many challenges to well-being, older adults in Mongolia reported high levels of flourishing. Those in urban areas had higher scores than those in rural areas and predictors of flourishing differed for these groups.
Behavior of medial gastrocnemius muscle beneath kinesio taping during isometric contraction and badminton lunge performance after fatigue induction
Authors: Vo Minh Hoang-Thuc , Lin Chien-Ju , Chieh Hsiao-Feng , Kuo Li-Chieh , An Kai-Nan , Wang Yu-Lin , Su Fong-Chin 
Published: January 2023

AbstractKinesio taping (KT) is widely used in sports for performance improvement and injury prevention. However, little is known of the behavior of the muscle region beneath the KT with movement, particularly when the muscle is fatigued. Accordingly, this study investigated the changes in the medial gastrocnemius muscle architecture and fascia thickness when using KT during maximum isometric plantar flexion (MVIC) and badminton lunges following heel rise exercises performed to exhaustion. Eleven healthy collegiate badminton players (4 males and 7 females) were recruited. All of the participants performed two tasks (MVIC and badminton lunge) with a randomized sequence of no taping, KT and sham taping and repeated following exhaustive repetitive heel rise exercise. In the MVIC task, the fascia thickness with the medial gastrocnemius muscle at rest significantly decreased following fatigue induction both without taping and with KT and sham taping (p = 0.036, p = 0.028 and p = 0.025, respectively). In the lunge task, the fascia thickness reduced after fatigue induction in the no taping and sham taping trials; however, no significant change in the fascia thickness occurred in the KT trials. Overall, the results indicate that KT provides a better effect during dynamic movement than in isometric contraction.
Induction of psoriasis- and atopic dermatitis-like phenotypes in 3D skin equivalents with a fibroblast-derived matrix
Authors: Morgner Bianka , Tittelbach Jörg , Wiegand Cornelia 
Published: January 2023

AbstractSkin homeostasis is a complex regulated process relying on the crosstalk of keratinocytes, fibroblasts and immune cells. Imbalances of T-cell subsets and the cytokine environment can lead to inflammatory skin diseases such as psoriasis (Ps) and atopic dermatitis (AD). Modern tissue engineering provides several in vitro models mimicking Ps and AD phenotypes. However, these models are either limited in their pathological features, life span, sample availability, reproducibility, controlled handling or simplicity. Some models further lack intensive characterization as they solely focus on differentiation and proliferation aspects. This study introduces a self-assembly model in which the pathological T-cell-signalling of Ps and AD was simulated by subcutaneous Th1 and Th2 cytokine stimulation. The self-established dermal fibroblast-derived matrices of these models were hypothesized to be beneficial for proximal cytokine signalling on epidermal keratinocytes. Comprehensive histological and mRNA analyses of the diseased skin models showed a weakened barrier, distinct differentiation defects, reduced cellular adhesion, inflammation and parakeratosis formation. A keratin shift of declining physiological cytokeratin-10 (CK10) towards increasing inflammatory CK16 was observed upon Th1 or Th2 stimulation. Antimicrobial peptides (AMPs) were upregulated in Ps and downregulated in AD models. The AD biomarker genes CA2, NELL2 and CCL26 were further induced in AD. While Ps samples featured basal hyperproliferation, cells in AD models displayed apoptotic signs. In accordance, these well-controllable three-dimensional in vitro models exhibited Ps and AD-like phenotypes with a high potential for disease research and therapeutic drug testing.
Association of KIR2DL5, KIR2DS5, and KIR2DS1 allelic variation and atopic dermatitis
Authors: Margolis David J. , Mitra Nandita , Hoffstad Ole J. , Berna Ronald , Kim Brian S. , Chopra Abha , Phillips Elizabeth J. 
Published: January 2023

AbstractNatural killer cells (NK) have been associated with the pathophysiology of atopic dermatitis (AD). NK function is regulated by killer cell Ig-like receptor family (KIR) receptors that interact with HLA ligands. The study goal was to focus on allelic variation in genes KIR2DL5, KIR2DS5, and KIR2DS1 with respect to AD. This was a case–control study of individuals with (n = 313) and without (n = 176) AD. Associations were estimated using logistic regression. The prevalence of KIR2DL5 was 52.5% (95% CI 48.0,57.0), KIR2DS5 was 33.0% (28.8,37.3), and KIR2DS1 was 33.6% (29.4,38.0). The presence of the KIR2DL5*001:01 increased the odds of having AD by about 86% (odds ratio (OR): 1.86(1.23,2.82) p = 0.003). The risk for individuals homozygous for KIR2DL5*001:01 was even greater (OR: 2.16 (95% CI 1.31,3.53) p = 0.0023). The odds of having AD with KIR2DL5*001:01 was similar in Whites and Blacks. Allelic variation in KIR2DS5 and KIR2DS1 was not associated with AD. There is no known HLA binding ligand for KIR2DL5. The effect of KIR2DL5*001:01 increased in the presence of HLA-B*-21TT leader sequence (2.46(1.37,4.41) p = 0.0025) and the HLA-C2 ligand (2.07 (1.37,4.41, p = 0.000002). Our study shows an independent association of the KIR2DL5*001:01 with AD and is the first study to associate AD with KIR allelic variation.
Modelling the long-term fairness dynamics of data-driven targeted help on job seekers
Authors: Scher Sebastian , Kopeinik Simone , Trügler Andreas , Kowald Dominik 
Published: January 2023

AbstractThe use of data-driven decision support by public agencies is becoming more widespread and already influences the allocation of public resources. This raises ethical concerns, as it has adversely affected minorities and historically discriminated groups. In this paper, we use an approach that combines statistics and data-driven approaches with dynamical modeling to assess long-term fairness effects of labor market interventions. Specifically, we develop and use a model to investigate the impact of decisions caused by a public employment authority that selectively supports job-seekers through targeted help. The selection of who receives what help is based on a data-driven intervention model that estimates an individual’s chances of finding a job in a timely manner and rests upon data that describes a population in which skills relevant to the labor market are unevenly distributed between two groups (e.g., males and females). The intervention model has incomplete access to the individual’s actual skills and can augment this with knowledge of the individual’s group affiliation, thus using a protected attribute to increase predictive accuracy. We assess this intervention model’s dynamics—especially fairness-related issues and trade-offs between different fairness goals- over time and compare it to an intervention model that does not use group affiliation as a predictive feature. We conclude that in order to quantify the trade-off correctly and to assess the long-term fairness effects of such a system in the real-world, careful modeling of the surrounding labor market is indispensable.
Mendelian randomization analyses of associations between breast cancer and bone mineral density
Authors: Wu Hong , Wang Hui , Liu Di , Liu Zhibing , Zhang Weiming 
Published: January 2023

AbstractThe purpose of this study was to verify whether there is a causal relationship between breast cancer and bone mineral density (BMD). Summary statistics for exposures and outcomes were obtained from corresponding genome-wide association studies. The bidirectional and multivariate mediated Mendelian randomization (MR) analyses were performed. In the bidirectional MR analysis, breast cancer might reduce the BMD of the heel (HE-BMD) (FDR = 1.51 × 10−4) as might its ER+ subtype (FDR = 1.51 × 10−4). From BMD to breast cancer, no significant association was found (FDR > 0.05). The mediating MR analysis showed that Higher free testosterone (FT) only mediated the causal relationship between breast cancer and HE-BMD by 2.9%; both ER+ type and FT were independent factors of HE-BMD (ER+: P = 0.021; FT: P = 6.88 × 10−6). Higher FT could increase the risk of breast cancer (FDR = 1.21 × 10−3) as could total testosterone (TT) (FDR = 5.81 × 10−3). Similarly, higher FT could increase the risk of ER+ subtype (FDR = 2.51 × 10−6) as could TT (FDR = 5.55 × 10−4). These results indicate that BMD is not a risk factor for breast cancer but breast cancer and its ER+ subtype are risk factors for BMD loss. Furthermore, higher FT and TT levels are associated with both an increased incidence of breast cancer and increased bone density.
Functional profiles of curatively treated adenoid cystic carcinoma unveil prognostic features and potentially targetable pathways
Authors: Romani Chiara , Lorini Luigi , Bozzola Anna , Bignotti Eliana , Tomasoni Michele , Ardighieri Laura , Bugatti Mattia , Battocchio Simonetta , Ravaggi Antonella , Tomasini Davide , Ravanelli Marco , Gurizzan Cristina , Lombardi Davide , Mattavelli Davide , Calza Stefano , Piazza Cesare , Bossi Paolo 
Published: January 2023

AbstractAdenoid cystic carcinoma (ACC) of salivary gland is a slowly growing tumor showing a propensity for delayed recurrence, with decreased survival rates. The identification of poor prognosis patients may help in defining molecular-based targeted strategies in this rare disease orphan of new treatments. Through a gene expression microarray-based approach followed by GSE functional analysis the expression profile of 46 primary untreated ACC samples and of ACC (h-TERT) tumor cells was analyzed. Patients who experienced early relapse showed enrichment in proliferation-related gene sets, including the G2-M checkpoint, E2F and myc targets, and in gene sets related to IFN signaling and aberrant proteostasis (FDR < 0.1), indicating increased mitotic and transcriptional activity in aggressive ACC. Similar functions were enriched in ACC samples classified by immunohistochemical staining as p63-negative, which exhibited increased protein burden and activation of pro-survival stress response pathways compared to p63-positive tumors. Compared to ACC tissues, ACC (h-TERT) cells share transcriptional features of aggressive p63-negative tumors. These data suggest association of specific pathway alterations with histopathological features of ACC, as recapitulated by p63 testing in patient prognostic stratification, anticipating new avenues for therapeutic intervention.
An objective absence data sampling method for landslide susceptibility mapping
Authors: Rabby Yasin Wahid , Li Yingkui , Hilafu Haileab 
Published: January 2023

AbstractThe accuracy and quality of the landslide susceptibility map depend on the available landslide locations and the sampling strategy for absence data (non-landslide locations). In this study, we propose an objective method to determine the critical value for sampling absence data based on Mahalanobis distances (MD). We demonstrate this method on landslide susceptibility mapping of three subdistricts (Upazilas) of the Rangamati district, Bangladesh, and compare the results with the landslide susceptibility map produced based on the slope-based absence data sampling method. Using the 15 landslide causal factors, including slope, aspect, and plan curvature, we first determine the critical value of 23.69 based on the Chi-square distribution with 14 degrees of freedom. This critical value was then used to determine the sampling space for 261 random absence data. In comparison, we chose another set of the absence data based on a slope threshold of < 3°. The landslide susceptibility maps were then generated using the random forest model. The Receiver Operating Characteristic (ROC) curves and the Kappa index were used for accuracy assessment, while the Seed Cell Area Index (SCAI) was used for consistency assessment. The landslide susceptibility map produced using our proposed method has relatively high model fitting (0.87), prediction (0.85), and Kappa values (0.77). Even though the landslide susceptibility map produced by the slope-based sampling also has relatively high accuracy, the SCAI values suggest lower consistency. Furthermore, slope-based sampling is highly subjective; therefore, we recommend using MD -based absence data sampling for landslide susceptibility mapping.