bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular vesicles engineering by silicates-activated endothelial progenitor cells for myocardial infarction treatment in male mice
Authors: Yu Bin , Li Hekai , Zhang Zhaowenbin , Chen Peier , Wang Ling , Fan Xianglin , Ning Xiaodong , Pan Yuxuan , Zhou Feiran , Hu Xinyi , Chang Jiang , Ou Caiwen 
Published: April 2023

AbstractExtracellular vesicles have shown good potential in disease treatments including ischemic injury such as myocardial infarction. However, the efficient production of highly active extracellular vesicles is one of the critical limitations for their clinical applications. Here, we demonstrate a biomaterial-based approach to prepare high amounts of extracellular vesicles with high bioactivity from endothelial progenitor cells (EPCs) by stimulation with silicate ions derived from bioactive silicate ceramics. We further show that hydrogel microspheres containing engineered extracellular vesicles are highly effective in the treatment of myocardial infarction in male mice by significantly enhancing angiogenesis. This therapeutic effect is attributed to significantly enhanced revascularization by the high content of miR-126a-3p and angiogenic factors such as VEGF and SDF-1, CXCR4 and eNOS in engineered extracellular vesicles, which not only activate endothelial cells but also recruit EPCs from the circulatory system.
Metabolomics analysis reveals metabolite changes during freeze-drying and oven-drying of Angelica dahurica
Authors: Wu Qinghua , Yan Qi , Jiang Lan , Chen Cuiping , Huang Xulong , Zhu Xinglong , Zhou Tao , Chen Jiang , Yan Jie , Wen Feiyan , Pei Jin 
Published: April 2023

AbstractAngelica dahurica (Angelica dahurica Fisch. ex Hoffm.) is widely used as a traditional Chinese medicine and the secondary metabolites have significant pharmacological activities. Drying has been shown to be a key factor affecting the coumarin content of Angelica dahurica. However, the underlying mechanism of metabolism is unclear. This study sought to determine the key differential metabolites and metabolic pathways related to this phenomenon. Liquid chromatography with tandem mass spectrometry (LC–MS/MS) based targeted metabolomics analysis was performed on Angelica dahurica that were freeze-drying (− 80 °C/9 h) and oven-drying (60 °C/10 h). Furthermore, the common metabolic pathways of paired comparison groups were performed based on KEEG enrichment analysis. The results showed that 193 metabolites were identified as key differential metabolites, most of which were upregulated under oven drying. It also displayed that many significant contents of PAL pathways were changed. This study revealed the large-scale recombination events of metabolites in Angelica dahurica. First, we identified additional active secondary metabolites apart from coumarins, and volatile oil were significantly accumulated in Angelica dahurica. We further explored the specific metabolite changes and mechanism of the phenomenon of coumarin upregulation caused by temperature rise. These results provide a theoretical reference for future research on the composition and processing method of Angelica dahurica.
Prevalence of long COVID complaints in persons with and without COVID-19
Authors: Magnusson Karin , Turkiewicz Aleksandra , Flottorp Signe Agnes , Englund Martin 
Published: April 2023

AbstractWe studied the prevalence and patterns of typical long COVID complaints in ~ 2.3 million individuals aged 18–70 years with and without confirmed COVID-19 in a Nation-wide population-based prospective cohort study in Norway. Our main outcome measures were the period prevalence of single-occurring or different combinations of complaints based on medical records: (1) Pulmonary (dyspnea and/or cough), (2) Neurological (concentration problems, memory loss), and/or (3) General complaints (fatigue). In persons testing positive (n = 75 979), 64 (95% confidence interval: 54 to 73) and 122 (111 to 113) more persons per 10 000 persons had pulmonary complaints 5–6 months after the test compared to 10 000 persons testing negative (n = 1 167 582) or untested (n = 1 084 578), respectively. The corresponding difference in prevalence of general complaints (fatigue) was 181 (168 to 195) and 224 (211 to 238) per 10 000, and of neurological complaints 5 (2 to 8) and 9 (6–13) per 10 000. Overlap between complaints was rare. Long COVID complaints were only slightly more prevalent in persons with than without confirmed COVID-19. Still, long COVID may pose a substantial burden to healthcare systems in the future given the lasting high incidence of symptomatic COVID-19 in both vaccinated and unvaccinated individuals.
GIS model for geothermal advantageous target selection
Authors: Li Xuan , Huang Changsheng , Chen Wei , Li Yanan , Han Jihong , Wang Xianguang , Bai Ximin , Yin Zhibin , Li Xiaozhe , Hou Pingping , Tong Jue 
Published: April 2023

AbstractAs the particularly popular green energy, geothermal resources are gradually favored by countries around the world, and the development model centered on geothermal dew point cannot meet the increasing geothermal demand. In this paper, a GIS model combining PCA and AHP is proposed, aiming to select the advantages of geothermal resources at the regional scale and analyze the main influencing indicators. Through the combination of the two methods, both data and empirical can be considered, then the geothermal advantage distribution on the area can be displayed through GIS software images. A multi-index evaluation system is established to qualitatively and quantitatively evaluate the mid-high temperature geothermal resources in Jiangxi Province, and carry out the evaluation of the dominant target areas and the analysis of geothermal impact indicators. The results show that it is divided into 7 geothermal resource potential areas and 38 geothermal advantage targets, and the determination of deep fault is the most critical index of geothermal distribution. This method is suitable for large-scale geothermal research, multi-index and multi-data model analysis and precise positioning of high-quality geothermal resource targets, which can meet the needs of geothermal research at the regional scale.
Performance analysis of the BDSBAS-B1C message in trial operation stage
Authors: Xin Jie , Guo Rui , Chen Jinping , Tian Yijun , Cao Yueling , Liu Yuchen , Li Xiaojie , Wang Dongxia , Cai Hongliang 
Published: April 2023

AbstractBeiDou Satellite-based Augmentation System (BDSBAS) has come into the trial operation stage since July, 2020. To evaluate the characteristic of the augmentation message in BDSBAS-B1C signal, the effectiveness of the message content was firstly analyzed, and then the validity of the broadcasting strategy was estimated. Finally, the accuracy of the user equivalent ranging error (UERE) and the single frequency positioning error with different correction parameters in BDSBAS-B1C message was evaluated. Based on the above analysis, the effectiveness of the augmentation message was preliminarily verified with the results showing that: (1) the BDSBAS-B1C message type, information content and update interval have basically met the international standard; (2) the accuracy of the UERE obtained with the augmentation message had an obvious improvement in contrast to that of the UERE obtained with the usual navigation message of the GPS satellites, and the ionospheric delay was one of the important factors which affected the accuracy of the UERE; (3) the positioning accuracy obtained with the augmentation message was also improved, and the improvement was more obvious in the service areas with high availability of the ionospheric parameters.
Chitosan-based nano-sorbents: synthesis, surface modification, characterisation and application in Cd (II), Co (II), Cu (II) and Pb (II) ions removal from wastewater
Authors: Chandra Dipesh , Molla Md. Tamzid Hossain , Bashar Md. Abul , Islam Md. Suman , Ahsan Md. Shameem 
Published: April 2023

AbstractIn contemplation of treating hazardous industrial wastewater, sodium tripolyphosphate (TPP) and vanillin (V)-modified chitosan-based magnetic nano-sorbents (TPP-CMN and V-CMN) were prepared, and the physical and surface properties of both nano-sorbents were characterised. The results of FE-SEM and XRD showed an average size of between 6.50 and 17.61 nm for Fe3O4 magnetic nanoparticles. The Physical Property Measurement System (PPMS) was carried out, and the saturation magnetisations for chitosan, Fe3O4 nanoparticles, TPP-CMN, and V-CMN were 0.153, 67.844, 7.211, and 7.772 emu.g−1, respectively. By using multi-point analysis, the BET surface areas of the synthesised TPP-CMN and V-CMN nano-sorbents were found to be 8.75 and 6.96 m2/g, respectively. The synthesised TPP-CMN and V-CMN were investigated as effective nano-sorbents to uptake Cd (II), Co (II), Cu (II), and Pb (II) ions, and the results were investigated by AAS. The adsorption process of heavy metals was investigated by the batch equilibrium technique, and the sorption capacity values of Cd (II), Co (II), Cu (II), and Pb (II) ions by TPP-CMN were 91.75, 93.00, 87.25, and 99.96 mg/g. By V-CMN, the values were 92.5, 94.00, 88.75, and 99.89 mg/g, respectively. The equilibrium times for adsorption were found to be 15 minutes for TPP-CMN and 30 minutes for V-CMN nano-sorbents. The adsorption isotherms, kinetics, and thermodynamics were studied to understand the adsorption mechanism. Furthermore, the adsorption of two synthetic dyes and two real wastewater samples was studied and obtained significant results. These nano-sorbents’ simple synthesis, high sorption capability, excellent stability, and recyclability may provide highly efficient and cost-effective nano-sorbents for wastewater treatment.
Properties of odor identification testing in screening for early-stage Alzheimer’s disease
Authors: Audronyte Egle , Pakulaite-Kazliene Gyte , Sutnikiene Vaiva , Kaubrys Gintaras 
Published: April 2023

AbstractOdor identification (OI) is impaired in the early stages of Alzheimer’s disease (AD). However, data regarding the diagnostic properties of OI tests are lacking, preventing their clinical use. We aimed to explore OI and determine the accuracy of OI testing in screening for patients with early AD. In total, 30 participants with mild cognitive impairment due to AD (MCI-AD), 30 with mild dementia due to AD (MD-AD), and 30 cognitively normal elderly participants (CN) were enrolled, and cognitive examination (CDR, MMSE, ADAS-Cog 13, and verbal fluency tests) and assessment of OI (Burghart Sniffin’ Sticks odor identification test) were performed. MCI-AD patients scored significantly worse in OI than CN participants, and MD-AD patients had worse OI scores than MCI-AD patients. The ratio of OI to ADAS-Cog 13 score had good diagnostic accuracy in differentiating AD patients from CN participants and in differentiating MCI-AD patients from CN participants. Substitution of ADAS-Cog 13 score with the ratio of OI to ADAS-Cog 13 score in a multinomial regression model improved the classification accuracy, especially of MCI-AD cases. Our results confirmed that OI is impaired during the prodromal stage of AD. OI testing has a good diagnostic quality and can improve the accuracy of screening for early-stage AD.
Laboratory simulation of the swampy forest system for the passive treatment of acid mine drainage in coal mine reclamation areas
Authors: Noor Ihsan , Arifin Yudi Firmanul , Priatmadi Bambang Joko , Saidy Akhmad Rizalli 
Published: April 2023

AbstractCoal mining that applies the open pit method has the potential to generate acid mine drainage. Acid mine drainage (AMD) treatments must include processes to mitigate significant challenges; these treatments include active treatment with high costs and process uncertainty and passive treatment with its limitations. The new concept of the swampy forest system involves the development of passive treatment for AMD, which lowers costs, increases capacity, and provides a natural process to mitigate the AMD that has been generated. A laboratory simulation experiment was carried out to obtain the basic data required for the swampy forest system treatment. The basic reference data determined in this study, including the total volume of water, the water debt flows into the swampy forest scale laboratory system and the retention time, were obtained to bring the parameter values that failed to meet the quality standards into compliance, according to the applicable regulations. The AMD swampy forest treatment design in the pilot project at the treatment field can apply a scaled-up version of the basic data from the simulation laboratory experiment results.
CZK, a novel alkaloid derivative from Clausena lansium, alleviates ischemic stroke injury through Nrf2-mediated antioxidant effects
Authors: Chen Haodong , Liu Yangbo , Feng Juling , Wang Hongyun , Yang Yantao , Ai Qidi , Zhang Zhao , Chu Shifeng , Chen Naihong 
Published: April 2023

AbstractAnti-oxidant stress is a potential strategy for the treatment of ischemic stroke. Here, we found a novel free radical scavenger termed as CZK, which is derived from alkaloids contained in Clausena lansium. In this study, we first compared cytotoxicity and biological activity between CZK and its parent’s compound Claulansine F. It was found that CZK had lower cytotoxicity and improved anti-oxygen–glucose deprivation/reoxygenation (OGD/R) injury than its parent’s compound. Free radical scavenging test showed that CZK had a strong inhibitory effect on hydroxyl free radicals with the IC50 of 77.08 nM. Intravenous injection of CZK (50 mg/kg) significantly alleviated ischemia–reperfusion injury, manifested by reduced neuronal damage and decreased oxidative stress. Consistent with the findings, the activities of superoxide dismutase (SOD) and reduced glutathione (GSH) were increased. Molecular docking predicted that CZK might be combined with nuclear factor erythroid 2-related factor 2 (Nrf2) complex. Our results also confirmed that CZK upregulated the contents of Nrf2 and its target gene products Heme Oxygenase-1 (HO-1), and NAD(P)H: Quinone Oxidoreductase 1 (NQO1). In conclusion, CZK had a potential therapeutic effect for ischemic stroke by activating Nrf2-mediated antioxidant system.
Heat variation on MHD Williamson hybrid nanofluid flow with convective boundary condition and Ohmic heating in a porous material
Authors: Rashad Ahmed M. , Nafe Mohamed A. , Eisa Dalia A. 
Published: April 2023

AbstractThe aim of the present study is to explore the variation of heat on MHD Williamson hybrid nanofluid (Ag-TiO2/H2O) model for steady two-dimensional and incompressible flow with a convective boundary condition in a curved coordinate porous system with Ohmic heating. Nusselt number is distinguished by the process of thermal radiation. The partial differential equations are controlled by the curved coordinate’s porous system, which depicts the flow paradigm. Employing similarity transformations, the acquired equations were turned into coupled non-linear ordinary differential equations. The governing equations were disbanded by RKF45 via shooting methodology. The focus is on examining physical characteristics such as heat flux at the wall, temperature distribution, velocity of flow, and surface friction coefficient for a variety of related factors. The analysis explained that increasing permeability, Biot and Eckert numbers enhance temperature profile and slowdown heat transfer. Moreover, convective boundary condition and thermal radiation enhance the friction of the surface. The model is prepared as an implementation for solar energy in processes of thermal engineering. Morever, this research has enormous applications in the industries of polymer and glass, also in the field of heat exchangers styling, cooling operations of metallic plates, etc.
Quantification of extracellular matrix remodeling for the non-invasive identification of graft fibrosis after liver transplantation
Authors: Engel Bastian , Falk Villesen Ida , Fisker Nielsen Mette Juul , Karsdal Morten , Taubert Richard , Jaeckel Elmar , Leeming Diana Julie 
Published: April 2023

AbstractDetecting patients with early post-transplant fibrosis after liver transplantation (LT) is very important. Non-invasive tests are needed to avoid liver biopsies. We aimed to detect fibrosis in liver transplant recipients (LTR) using extracellular matrix (ECM) remodeling biomarkers. ECM biomarkers for type III (PRO-C3), IV (PRO-C4), VI (PRO-C6) and XVIII (PRO-C18L) collagen formation and type IV collagen degradation (C4M) were measured by ELISA in prospectively collected, cryopreserved plasma samples (n = 100) of LTR with paired liver biopsies from a protocol biopsy program. Fibrosis ≥ F2 was present in 29% of patients (median 44 months post-LT). APRI and FIB-4 neither identified significant fibrosis nor were correlated with histopathological fibrosis scores, while ECM biomarkers (AUCs 0.67–0.74) did. The median levels of PRO-C3 (15.7 vs. 11.6 ng/ml; p = 0.002) and C4M (22.9 vs. 11.6 ng/ml; p = 0.006) levels were elevated in T-cell-mediated rejection compared to normal graft function. The median levels of PRO-C4 (178.9 vs. 151.8 ng/ml; p = 0.009) and C4M (18.9 vs. 16.8 ng/ml; p = 0.004) levels were increased if donor-specific antibodies were present. PRO-C6 had the highest sensitivity (100%), NPV (100%) and negative likelihood-ratio (0) for graft fibrosis. To conclude, ECM biomarkers are helpful in identifying patients at risk of relevant graft fibrosis.
Association between the Cardiac Arrest Hospital Prognosis (CAHP) score and reason for death after successfully resuscitated cardiac arrest
Authors: Paul Marine , Legriel Stéphane , Benghanem Sarah , Abbad Sofia , Ferré Alexis , Lacave Guillaume , Richard Olivier , Dumas Florence , Cariou Alain 
Published: April 2023

AbstractIndividualize treatment after cardiac arrest could potentiate future clinical trials selecting patients most likely to benefit from interventions. We assessed the Cardiac Arrest Hospital Prognosis (CAHP) score for predicting reason for death to improve patient selection. Consecutive patients in two cardiac arrest databases were studied between 2007 and 2017. Reasons for death were categorised as refractory post-resuscitation shock (RPRS), hypoxic-ischaemic brain injury (HIBI) and other. We computed the CAHP score, which relies on age, location at OHCA, initial cardiac rhythm, no-flow and low-flow times, arterial pH, and epinephrine dose. We performed survival analyses using the Kaplan–Meier failure function and competing-risks regression. Of 1543 included patients, 987 (64%) died in the ICU, 447 (45%) from HIBI, 291 (30%) from RPRS, and 247 (25%) from other reasons. The proportion of deaths from RPRS increased with CAHP score deciles; the sub-hazard ratio for the tenth decile was 30.8 (9.8–96.5; p < 0.0001). The sub-hazard ratio of the CAHP score for predicting death from HIBI was below 5. Higher CAHP score values were associated with a higher proportion of deaths due to RPRS. This score may help to constitute uniform patient populations likely to benefit from interventions assessed in future randomised controlled trials.
Real-time gas mass spectroscopy by multivariate analysis
Authors: Franceschelli Leonardo , Ciricugno Carla , Di Lorenzo Maurizio , Romani Aldo , Berardinelli Annachiara , Tartagni Marco , Correale Raffaele 
Published: April 2023

AbstractEarly and significant results for a real-time, column-free miniaturized gas mass spectrometer in detecting target species with partial overlapping spectra are reported. The achievements have been made using both nanoscale holes as a nanofluidic sampling inlet system and a robust statistical technique. Even if the presented physical implementation could be used with gas chromatography columns, the aim of high miniaturization requires investigating its detection performance with no aid. As a study case, in the first experiment, dichloromethane (CH2Cl2) and cyclohexane (C6H12) with concentrations in the 6–93 ppm range in single and compound mixtures were used. The nano-orifice column-free approach acquired raw spectra in 60 s with correlation coefficients of 0.525 and 0.578 to the NIST reference database, respectively. Then, we built a calibration dataset on 320 raw spectra of 10 known different blends of these two compounds using partial least square regression (PLSR) for statistical data inference. The model showed a normalized full-scale root-mean-square deviation (NRMSD) accuracy of $$10.9\mathrm{\%}$$

10.9
%

and $$18.4\mathrm{\%}$$

18.4
%

for each species, respectively, even in combined mixtures. A second experiment was conducted on mixes containing two other gasses, Xylene and Limonene, acting as interferents. Further 256 spectra were acquired on 8 new mixes, from which two models were developed to predict CH2Cl2 and C6H12, obtaining NRMSD values of 6.4% and 13.9%, respectively.
The benefit of planned dialysis to early survival on hemodialysis versus peritoneal dialysis: a nationwide prospective multicenter study in Korea
Authors: Lim Jeong-Hoon , Kim Ji Hye , Jeon Yena , Kim Yon Su , Kang Shin-Wook , Yang Chul Woo , Kim Nam-Ho , Jung Hee-Yeon , Choi Ji-Young , Park Sun-Hee , Kim Chan-Duck , Kim Yong-Lim , Cho Jang-Hee 
Published: April 2023

AbstractOptimal preparation is recommended for patients with advanced chronic kidney disease to minimize complications during dialysis initiation. This study evaluated the effects of planned dialysis initiation on survival in patients undergoing incident hemodialysis and peritoneal dialysis. Patients newly diagnosed with end-stage kidney disease who started dialysis were enrolled in a multicenter prospective cohort study in Korea. Planned dialysis was defined as dialysis therapy initiated with permanent access and maintenance of the initial dialysis modality. A total of 2892 patients were followed up for a mean duration of 71.9 ± 36.7 months and 1280 (44.3%) patients initiated planned dialysis. The planned dialysis group showed lower mortality than the unplanned dialysis group during the 1st and 2nd years after dialysis initiation (1st year: adjusted hazard ratio [aHR] 0.51; 95% confidence interval [CI] 0.37–0.72; P < 0.001; 2nd year: aHR 0.71; 95% CI 0.52–0.98, P = 0.037). However, 2 years after dialysis initiation, mortality did not differ between the groups. Planned dialysis showed a better early survival rate in hemodialysis patients, but not in peritoneal dialysis patients. Particularly, infection-related mortality was reduced only in patients undergoing hemodialysis with planned dialysis initiation. Planned dialysis has survival benefits over unplanned dialysis in the first 2 years after dialysis initiation, especially in patients undergoing hemodialysis. It improved infection-related mortality during the early dialysis period.