bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dominant Expression of Chromogranin B in Pituitary Corticotrophs and Its Putative Role in Interaction With Secretogranin III
Authors: Kikuchi Shota , Odashima Koki , Yasui Tadashi , Torii Seiji , Hosaka Masahiro , Gomi Hiroshi 
Published: January 2025

Summary Previous studies have suggested that chromogranin A (CgA) is a partner molecule of secretogranin III (SgIII). In mouse pituitary corticotroph-derived AtT-20 cells, SgIII plays a role in sorting CgA/hormone aggregates into secretory granules (SGs). Although CgA expression is equivocal, CgB is clearly detectable in the rat pituitary corticotrophs. Therefore, we hypothesized that CgB shares a function with CgA in pituitary corticotrophs. In the binding assays, CgB, similar to CgA, showed binding activity to SgIII under weakly acidic conditions and in the presence of Ca2+. Considering the differences in animal species, the different abilities of antibodies, and the conditions of tissue fixation and thin sectioning in immunofluorescence histochemistry, we found that CgA was expressed in a small population (approximately 10%), and its expression intensity was weaker than that of CgB (>98%) in rodent pituitary corticotrophs. In addition, similar to CgA, CgB and SgIII were colocalized in adrenocorticotropic hormone (ACTH) granules. The labeling of CgA and CgB was not completely consistent, and CgB colocalized with SgIII in many granules. These results suggest that there are multiple sorting systems for ACTH granules in pituitary corticotrophs and that the SgIII/CgB complex behaves more dominantly than the SgIII/CgA complex, which has somewhat different properties:
A robust transfer learning approach for high-dimensional linear regression to support integration of multi-source gene expression data
Authors: Pan Lulu , Gao Qian , Wei Kecheng , Yu Yongfu , Qin Guoyou , Wang Tong 
Published: January 2025

Transfer learning aims to integrate useful information from multi-source datasets to improve the learning performance of target data. This can be effectively applied in genomics when we learn the gene associations in a target tissue, and data from other tissues can be integrated. However, heavy-tail distribution and outliers are common in genomics data, which poses challenges to the effectiveness of current transfer learning approaches. In this paper, we study the transfer learning problem under high-dimensional linear models with t-distributed error (Trans-PtLR), which aims to improve the estimation and prediction of target data by borrowing information from useful source data and offering robustness to accommodate complex data with heavy tails and outliers. In the oracle case with known transferable source datasets, a transfer learning algorithm based on penalized maximum likelihood and expectation-maximization algorithm is established. To avoid including non-informative sources, we propose to select the transferable sources based on cross-validation. Extensive simulation experiments as well as an application demonstrate that Trans-PtLR demonstrates robustness and better performance of estimation and prediction when heavy-tail and outliers exist compared to transfer learning for linear regression model with normal error distribution.
Data integration, Variable selection, T distribution, Expectation maximization algorithm, Genotype-Tissue Expression, Cross validation.
Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections
Authors: Pilotte Nils , Omballa Victor , Voss Monica , Padgett Leah , Manuel Malathi , Goodman Jeanne L. , Littlewood Tim , Manoharan Zayina Zondervenni , Lieshout Lisette van , Verweij Jaco J. , Sekar Manigandan , Muthukumar Ajith Kumar , Walch Gretchen , Warren Abriana , Sheikh Mariyam , Gonzalez Andrew , Galagan Sean R. , Ajjampur Sitara Swarna Rao , Ibikounlé Moudachirou , Williams Steven A. , Rains Doug , Dadwal Ushashi , Walson Judd L. 
Published: January 2025

Background
Historically, soil-transmitted helminth (STH) control and prevention strategies have relied on mass drug administration efforts targeting preschool and school-aged children. While these efforts have succeeded in reducing morbidity associated with STH infection, recent modeling efforts have suggested that expanding intervention to treatment of the entire community could achieve transmission interruption in some settings. Testing the feasibility of such an approach requires large-scale clinical trials, such as the DeWorm3 cluster randomized trial. In addition, accurate interpretation of trial outcomes requires diagnostic platforms capable of accurately determining infection prevalence (particularly as infection intensity is reduced) at large population scale and with significant throughput. Here, we describe the development and validation of such a high-throughput molecular testing platform.

Methodology/principal findings
Through the development, selection, and validation of appropriate controls, we have successfully created and evaluated the performance of a testing platform capable of the semi-automated, high-throughput detection of four species of STH in human stool samples. Comparison of this platform with singleplex reference assays for the detection of these same pathogens has demonstrated comparable performance metrics, with index assay accuracy measuring at or above 99.5% and 98.1% for each target species at the level of the technical replicate and individual extraction respectively. Through the implementation of a rigorous validation program, we have developed a diagnostic platform capable of providing the necessary throughput and performance required to meet the needs of the DeWorm3 cluster randomized trial and other large-scale operational research efforts for STH.

Conclusions/significance
Resulting from the rigorous developmental approach taken, the platform we describe here provides the needed confidence in testing outcomes when utilized in conjunction with large-scale efforts such as the DeWorm3 trial. Additionally, the successful development of an evaluation and validation strategy provides a template for the creation of similar diagnostic platforms for other neglected tropical diseases.
Height development and multiple bone health indicators in children aged 2–12 years with Duchenne muscular dystrophy (DMD)
Authors: Wang Bingying , Zhou Linyuhan , Li Shuangru , Xu Huayan , Guo Yingkun , Hu Qin , Huang Min , Zhou Dan , Cai Xiaotang , Wang Qiu , Sun Xiaomei 
Published: January 2025

Introduction
Short stature is a frequent complication of DMD, and its pathomechanisms and influencing factors are specific to this disease and the idiosyncratic treatment for DMD.

Purpose
To establish the height growth curve of early DMD, and evaluate the potential influencing markers on height growth, provide further evidence for pathological mechanism, height growth management and bone health in DMD.

Methods
A retrospective, cross-sectional study of 348 participants with DMD aged 2–12 years was conducted at West China Second Hospital of Sichuan University from January 2023 to October 2023.

Results
The growth curve for 2–12 years old boys with DMD indicates a slower growth rate compared to the average population. At age two, children with DMD have a similar height to their peers, but gradually falls behind afterwards. Short stature was observed in children with DMD before and after GC exposure, and prolonged GC use exacerbated the retardation. BMI (β = -0.47, p = 0.007), BMD (β = -0.005, p = 0.014), β-CTX (β = 0.001, p = 0.002), delayed BA (β = 0.417, p < .001), GC duration (β = -0.006, p = 0.047) were independent influencing factors of height. Relevant bone health markers showed different sequential changing patterns.

Conclusion
The high proportion and progression of short stature are associated with the broad bone health status. Different bone indicators have different sensitivities and specificities and need to be considered together for clinical monitoring of bone health. This study provides evidence for the early monitoring of height development and relevant factors as part of bone health management in DMD, to minimize the occurrence of bone-related complications later in life.
A national survey of career development according to gender and subspecialties among cardiologists in Japan
Authors: Shimbo Mai , Nakayama Atsuko , Fukue Noriko , Nishizaki Fumie , Matsumoto Chisa , Noma Satsuki , Ohno-Urabe Satoko , Kamiya Chizuko A. , Kanki Sachiko , Ide Tomomi , Izawa Hideo , Taniguchi Tatsunori , Kobayashi Yoshio 
Published: January 2025

Background
Training opportunities, work satisfaction, and the factors that influence them according to gender and subspecialties are understudied among Japanese cardiologists.

Methods
We investigated the career development of Japanese cardiologists with an e-mail questionnaire. Feelings of inequality in training opportunities, work dissatisfaction, and reasons were assessed by examining the cardiologists’ gender and invasiveness of subspecialties.

Results
Responses were received from 2,566 cardiologists. Female cardiologists were underrepresented in invasive subspecialties compared to males (14.2% vs. 85.8%, p<0.0001). In both invasive and non-invasive subspecialties, female cardiologists felt more inequality in training opportunities than males (invasive: 50.0% vs. 36.2%, non-invasive: 41.6% vs. 30.9%, p<0.001, respectively) and were less satisfied with their work (invasive: 26.0% vs. 18.3%, non-invasive: 24.7% vs. 14.7%, p = 0.001, respectively). Although female cardiologists in invasive subspecialties did not feel significantly more inequal and dissatisfied than those in non-invasive subspecialties (p = 0.063 and p = 0.758, respectively), male cardiologists in invasive subspecialties felt more inequal and dissatisfied than those in non-invasive subspecialties (p = 0.015 and p = 0.040, respectively). Female cardiologists were more influenced by gender bias and family issues for inequality in training opportunities (p = 0.0001, respectively), whereas male cardiologists were likely to be affected by specifications of belonging hospitals. Both genders felt dissatisfied when their expectations were unmet and they were overworked.

Conclusions
Female cardiologists felt more inequality regarding training opportunities and dissatisfaction with career development than male cardiologists in both the invasive and non-invasive subspecialties. Diversity support is warranted for achieving satisfying career course regardless of gender and subspecialty.
Situational Syncope Triggered by Swallowing
Authors: Ellington Abigail Layton , Tarry Samuel Thierjung , Ellis Matthew Nigel Hugh , Clayton Steven B. 
Published: January 2025

ABSTRACT
Syncope is characterized by a transient loss of consciousness. Swallow syncope, a rare cause of syncope, is caused by vagus nerve activation resulting in vasodilation and bradycardia, thus causing transient hypotension and cerebral hypoperfusion. It is diagnosed through clinical history, cardiac, and esophageal evaluation. We present a case of swallow syncope in a patient with significant cardiac history. Initial cardiac and esophageal testing was normal. Long-term telemetry revealed bradycardia with swallowing, and the patient underwent pacemaker placement. Swallow syncope is associated with a variety of esophageal and cardiac conditions. Management involves addressing the underlying cause; pacemaker placement is sometimes necessary.
KEYWORDS: syncope; swallow syncope; reflex syncope; pacemaker
Sex differences in exercise‐induced arterial hypoxemia and pulmonary edema following high‐intensity exercise in highly trained endurance athletes
Authors: Hind Alanna S. , Mitchell Reid A. , Ferguson Olivia N. , Flynn Morgan , Dhillon Satvir S. , Badra Karine , Milne Kathryn M. , Iannetta Danilo , Koehle Michael S. , Guenette Jordan A. 
Published: January 2025

AbstractThis study investigated sex differences in the development of pulmonary edema and exercise‐induced arterial hypoxemia (EIAH) in well‐trained endurance athletes during near‐maximal exercise in a real‐world setting. Twenty participants (10M vs. 10F; V̇O2peak: 69.3 (8.8) vs. 50.7 (4.1) ml∙kg−1∙min−1) underwent a maximal incremental treadmill test (visit 1) and a time trial on a steep trail (~2.5 km, ~800 m elevation gain) in North Vancouver (visit 2). Pulmonary edema was evaluated using handheld lung ultrasound ~10–15 min post‐exercise and oxygen saturation (SpO2) was monitored using finger pulse oximetry. Males completed the time trial significantly faster than females (M: 31.5 (6.5) vs. F: 40.4 (7.5) min, p = 0.006), while females sustained a higher percentage of their visit 1 heart rate (M: 94 (1) vs. F: 96 (1) %max, p = 0.02). All participants developed EIAH, with no sex differences in end‐exercise SpO2 (M: 89 (4) % vs. F: 90 (3) %, respectively, p = 0.35). There was no evidence of pulmonary edema, assessed through ultrasound b‐line scores, with no differences between sexes (M: 0.3 (1.0) vs. F: 0.5 (1.5), respectively, p = 0.60). Pulmonary edema is an unlikely contributor to EIAH in endurance athletes performing near‐maximal time trial exercise in a real‐world setting.
The crystal structure of N-(3-bromo-4-fluorophenyl)-N′-hydroxy-4-{[2-(4-methylphenyl)ethyl]amino}-1,2,5-oxadiazole-3-carboximidamide, C18H17BrFN5O2
Authors: Zuo Limin , Zhou Xia , Liu Yitong , Gu Yongsheng , Jiang Yifei , Lian Xiaofang , Liu Huiyi , Jia Qingying , Shan Guangzhi 
Published: January 2025

Abstract
C18H17BrFN5O2, monoclinic, P21/c (no. 14), a = 19.3571(8) Å, b = 13.4295(12) Å, c = 7.3036(5) Å, β = 93.372(5)°, V = 1895.3(2) Å3, Z = 4, Rgt
(F) = 0.0407, wRref
(F
2) = 0.0939, T = 293(2) K.
Synthesis and crystal structure of ethyl (2S,4aS,6aS,6bR,8aR,12aS,12bR,14bR,E)-10-(((3,4-dichlorobenzyl)oxy)imino)-2,4a,6a,6b,9,9,12a-heptamethyl-13-oxo-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-2-carboxylate
Authors: Dong Zhang-Chao , Shen Hui , Mu Ze-Bo , Xu Kai-Hua 
Published: January 2025

Abstract
C39H53Cl2NO4, monoclinic, P21 (no. 4), a = 7.55730(10) Å, b = 11.0989(2) Å, c = 20.9222(3) Å, β = 91.426(1)°, V = 1754.36(5) Å3, Z = 2, R

gt
(F) = 0.0360, wR

ref
(F
2) = 0.1028, T = 169.98(10) K.
Impact of permeability and fluid parameters in couple stress media on rotating eccentric spheres
Authors: Al-Hanaya Amal , El-Sapa Shreen 
Published: January 2025

Abstract
This study elucidates the axisymmetric rotation of two eccentric spheres immersed in an incompressible couple stress fluid within a porous annular region. The investigation incorporates boundary conditions applied to the surfaces of both spheres, which rotate axially at differing angular velocities. Utilizing a collocation scheme alongside a semi-analytical approach under the assumption of low Reynolds numbers, this research delves into the hydrodynamic couple force (torque) exerted by the Brinkman couple stress fluid on the interior sphere. The analysis reveals that the dimensionless torque becomes increasingly significant as permeability enhances the size ratio, couple stress fluid parameters, and separation distance. The computed torque values due to stress and couple stress are presented graphically, providing visual clarity to the findings. Additionally, all the results align closely with the numerical analysis of two eccentric rigid spheres in conventional couple stress fluids, devoid of permeability effects or slip conditions, as demonstrated in previous studies (Al-Hanaya and El-Sapa (Effects of slippage and permeability of couple stress fluid squeezed between two concentric rotating spheres. Phys Fluids. 2023;35:103112); Al-Hanaya et al. (Axisymmetric motion of an incompressible couple stress fluid between two eccentric rotating spheres. J Appl Mech Tech Phys. 2022;63(5):1–9)).
Pleural metastases from differentiated thyroid cancer: clinical features and long-term outcomes
Authors: Liu Mengyue , Tang Juan , Sun Nan , Xi Chuang , Shen Chentian , Hongjun Song , Luo Quanyong , Deng Xianzhao , Qiu Zhongling 
Published: January 2025

Objective
Pleural metastasis (PM) is rare in patients with differentiated thyroid cancer (DTC). Radioiodine (131I) therapy has been the main treatment for postoperative metastasis and recurrence of DTC. However, clinical data on PM from DTC are limited. This study investigated the clinicopathological characteristics of patients with PM from DTC that were treated surgically and with 131I therapy and evaluated their long-term prognosis and prognostic factors.

Methods
A review of the Shanghai Sixth People’s Hospital medical records from 2010 to 2023 identified PM in 27 of 14,473 patients with DTC. Overall survival (OS) was assessed by the Kaplan–Meier method.

Results
The prevalence of PM in DTC was 1.87‰ (27/14,473). The median age at the time of initial diagnosis of PM was 59 years (range: 34–79). At the end of follow-up, eight patients (29.63%) had disease progression (PD), four (14.81%) had a partial response, and the remainder had stable disease; no patient achieved complete response. Twelve patients (44.44%) died, and 15 (55.56%) survived. Thirteen patients (48.15%) did not show 131I avidity, and 16 (59.26%) had radioiodine-refractory DTC (RR-DTC). Twenty patients (74.07%) had malignant pleural effusion (MPE), which was large in 11 cases (40.74%) and moderate in two. More-than-moderate MPE (P = 0.031), lack of 131I avidity (P = 0.041) and RR-DTC (P = 0.030) were significantly associated with worse 5-year OS in patients with PM of DTC.

Conclusions
PM is rare in DTC. Lack of 131I avidity, RR-DTC and more-than-moderate MPE are associated with poor OS in patients with DTC and PM.
IMPLEMENTASI HEAD-UP ELEVASI 30° PADA PASIEN STROKE DENGAN RISIKO PERFUSI SEREBRAL TIDAK EFEKTIF
Authors: Khasanah Yunnik Fatun , Fibriansari Rizeki Dwi , Astuti Anggia 
Published: January 2025

yang mengarah ke otak yang menyebabkan gangguan serebral. Stroke terjadi pada aliran darah ke otak terhambat secara mendadak selama lebih dari 24 jam karena pembuluh darah mengalami penyumbatan atau pecah. Hal ini dapat terjadi akibat beberapa faktor diantaranya tekanan darah yang tidak terkontrol, nyeri kepala, pusing, dan mual muntah. Untuk mengatasi sebagian masalah tersebut maka diperlukan terapi nonfarmakologi yaitu pemberian posisi head-up elevasi 30° dengan risiko perfusi serebral dapat efektif. Tujuan mengeksplorasi asuhan keperawatan medikal bedah menggunakan implementasi head-up elevasi pada pasien stroke dengan masalah keperawatan risiko perfusi serebral tidak efektif.
Penelitian ini menggunakan desain studi kasus yang dilakukan pada satu responden dengan kriteria inklusi usia diatas 50 tahun dan faktor risiko hipertensi. Penelitian ini dilakukan selama 3 hari di Ruang Kenanga RSUD dr. Haryoto Lumajang pada bulan Juni 2024. Pengumpulan data menggunakan teknik wawancara, observasi dan dokumentasi dengan intervensi penentu. Alat atau instrumen yang digunakan adalah SOP head-up dan lembar observasi. Implementasi head-up elevasi 30° yang dilakukan 30 menit selama tiga hari pada pasien stroke yang mengalami risiko perfusi serebral tidak efektif dengan nilai tekanan darah awal 161/101 mmHg sampai tekanan darah akhir 141/58 mmHg dan hasil MAP awal 121 mmHg dan MAP akhir 85,6 mmHg.
Perawat disarankan dapat menggunakan sebagai pedoman kerja yang digunakan pada pasien stroke dengan masalah risiko perfusi serebral tidak efektif melalui penerapan intervensi head-up elevasi 30°. Dengan demikian, pemberian terapi head-up elevasi 30° berpengaruh dalam penurunan tekanan intrakranial tujuannya untuk mempengaruhi venous return menjadi maksimal sehingga aliran darah ke serebral menjadi lancar, meningkatkan metabolisme jaringan serebral dan memaksimalkan oksigenasi jaringan otak, sehingga otak.
The Quantitative Characteristics of Cross-Bred Native Chicken (Sentul X Arab Chicken and Merawang X Arab Chicken)
Authors: Irmaya. H Dika , Suyadi Suyadi , Depison Depison , Ardiantoro Ari 
Published: January 2025

The research aims to analyze the production potential and opportunities to increase the productivity of each cross-bred animal based on its quantitative characteristics. The livestock material used in this research was 50 Sera chickens (Sentul chickens x Arabian chickens) and 50 Mera chickens (Merawang chickens x Arabian chickens). The research was carried out by raising chickens from DOC to 3 months of age. The quantitative data involve body weight, weight gain, and body measurements (Head Length, Head Circumference, Head Height, Neck Length, Neck Circumference, Wing Length, Back Length, Back Height, Chest Length, Chest Width, Shank Length, Shank Circumference, Length Third Finger and Pubic Bone Distance). The quantitative data that has been observed is then analyzed through the t-test. Then, PCA is used to identify body shape and size determinants in Sera and Mera chickens. Statistical analysis was carried out using Minitab software version 21. The results obtained showed that the body weights of Sera and Mera chickens were significantly different (p<0.05) from DOC to 3 months of age. A significant increase in productivity in crosses between Sentul chickens and Arabian chickens and Merawang chickens with Arabian chickens occurs starting at 1-2 months. Sera and Mera chickens' body sizes differed significantly (p<0.05). The total diversity of PC1 in Sera chickens was 79.8%, and in Mera chickens, it was 70.6%. Meanwhile, the total PC2 diversity of Sera chickens was 17.7%, and Mera chickens were 7.9%. In conclusion, Sera chickens have the highest quantitative characteristics compared to Mera chickens, and the highest body weight gain occurs at 1-2 months in both Sera and Mera chickens. Based on principal component analysis. The body size characteristic of Sera chickens is shank length, while in Mera chickens, it is chest length. The distinct shape of Sera chickens is body height, while in Mera chickens, it is shank length.
Evaluating the Use of Marlin Fish (Istiompax indica) By-product Meal in the Ration on Carcass and Organ Yields of Broiler Chickens
Authors: Zulfan Zulfan Zulfan , Daud Muhammad , Latif Herawati , Khairunnisa Novia , Anggraini Tantri 
Published: January 2025

This study aimed to examine the effect of marlin fish (Istiompax indica) byproduct meal (MFBM) mixed with some other feed ingredients such as yellow corn (YC), rice bran (RB), broken rice (BR), and top mix (TM) as a mixture feed (MF) in replacement fractionally a commercial ration (CR) on carcasses and organs of broiler chickens. As a comparison, commercial local fish meal (CLFM) was used instead of MFBM in the MF. A total of 100 DOCs, strain MB 202, were raised for 35 days of age to feed the experimental rations. The experimental rations were: CR= 100% CP511/512 (control), MFBM4= 80% CP511/512 + 20% MF1 containing 4% MFBM, MFBM8= 80% CP511/512 + 20% MF2 containing 8% MFBM, CLFM4= 80% CP511/512 + 20% MF3 containing 4% CLFM, and CLFM8= 80% CP511/512 + 20% MF4 containing 8% CLFM. The research used a completely randomized design (CRD) with five treatments, four replications, and two subsamples. Data were analyzed by Analysis of Variance (ANOVA) and continued by Duncan’s multiple range taste (DMRT) only if the results detected a significant difference. This study showed that at 4% usage within the MF, using MFBM did not indicate a significant difference in the whole carcass and cuts-up weights than using CLFM. However, at the level of 8% within an MF, the former was significantly higher (p<0.05) on the whole carcass and breast weight than the latter. In conclusion, marlin fish byproduct meal (MFBM) could be used up to 8% to produce better whole carcass and breast weight compared to commercial local fish meal (CLFM) without adverse effects on the internal organs of broilers.