bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age‐Associated Changes in Knee Osteoarthritis, Pain‐Related Behaviors, and Dorsal Root Ganglia Immunophenotyping of Male and Female Mice
Authors: Geraghty Terese , Obeidat Alia M. , Ishihara Shingo , Wood Matthew J. , Li Jun , Lopes Erika Barboza Prado , Scanzello Carla R. , Griffin Timothy M. , Malfait Anne‐Marie , Miller Rachel E. 
Published: April 2023

ObjectiveOsteoarthritis (OA) is a leading cause of chronic pain, yet OA pain management remains poor. Age is the strongest predictor of OA development, and mechanisms driving OA pain are unclear. We undertook this study to characterize age‐associated changes in knee OA, pain‐related behaviors, and dorsal root ganglion (DRG) molecular phenotypes in mice of both sexes.MethodsMale or female C57BL/6 mice 6 or 20 months of age were evaluated for histopathologic knee OA, pain‐related behaviors, and L3–L5 DRG immune characterization via flow cytometry. DRG gene expression in older mice and humans was also examined.ResultsMale mice at 20 months of age had worse cartilage degeneration than 6‐month‐old mice. Older female mouse knees showed increased cartilage degeneration but to a lesser degree than those of male mice. Older mice of both sexes had worse mechanical allodynia, knee hyperalgesia, and grip strength compared to younger mice. For both sexes, DRGs from older mice showed decreased CD45+ cells and a significant increase in F4/80+ macrophages and CD11c+ dendritic cells. Older male mouse DRGs showed increased expression of Ccl2 and Ccl5, and older female mouse DRGs showed increased Cxcr4 and Ccl3 expression compared to 6‐month‐old mouse DRGs, among other differentially expressed genes. Human DRG analysis from 6 individuals >80 years of age revealed elevated CCL2 in men compared to women, whereas CCL3 was higher in DRGs from women.ConclusionWe found that aging in male and female mice is accompanied by mild knee OA, mechanical sensitization, and changes to immune cell populations in the DRG, suggesting novel avenues for development of OA therapies.image
Lysosome‐Associated Membrane Protein 3 Induces Lysosome‐Dependent Cell Death by Impairing Autophagic Caspase 8 Degradation in the Salivary Glands of Individuals With Sjögren's Disease
Authors: Nakamura Hiroyuki , Tanaka Tsutomu , Zheng Changyu , Afione Sandra A. , Warner Blake M. , Noguchi Masayuki , Atsumi Tatsuya , Chiorini John A. 
Published: April 2023

ObjectiveLysosome‐associated membrane protein 3 (LAMP3) overexpression is implicated in the development and progression of Sjögren's disease (SjD) by inducing lysosomal membrane permeabilization (LMP) and apoptotic cell death in salivary gland epithelium. The aim of this study was to clarify the molecular details of LAMP3‐induced lysosome‐dependent cell death and to test lysosomal biogenesis as a therapeutic intervention.MethodsHuman labial minor salivary gland biopsies were analyzed using immunofluorescence staining for LAMP3 expression levels and galectin‐3 puncta formation, a marker of LMP. Expression level of caspase 8, an initiator of LMP, was determined by Western blotting in cell culture. Galectin‐3 puncta formation and apoptosis were evaluated in cell cultures and a mouse model treated with glucagon‐like peptide 1 receptor (GLP‐1R) agonists, a known promoter of lysosomal biogenesis.ResultsGalectin‐3 puncta formation was more frequent in the salivary glands of SjD patients compared to control glands. The proportion of galectin‐3 puncta–positive cells was positively correlated with LAMP3 expression levels in the glands. LAMP3 overexpression increased caspase 8 expression, and knockdown of caspase 8 decreased galectin‐3 puncta formation and apoptosis in LAMP3‐overexpressing cells. Inhibition of autophagy increased caspase 8 expression, while restoration of lysosomal function using GLP‐1R agonists decreased caspase 8 expression, which reduced galectin‐3 puncta formation and apoptosis in both LAMP3‐overexpressing cells and mice.ConclusionLAMP3 overexpression induced lysosomal dysfunction, resulting in lysosome‐dependent cell death via impaired autophagic caspase 8 degradation, and restoring lysosomal function using GLP‐1R agonists could prevent this. These findings suggested that LAMP3‐induced lysosomal dysfunction is central to disease development and is a target for therapeutic intervention in SjD.image
Risk–Benefit Analysis of Primary Prophylaxis Against Pneumocystis Jirovecii Pneumonia in Patients With Rheumatic Diseases Receiving Rituximab
Authors: Park Jun Won , Curtis Jeffrey R. , Choi Se Rim , Kim Min Jung , Ha You‐Jung , Kang Eun Ha , Lee Yun Jong , Lee Eun Bong 
Published: April 2023

ObjectiveTo identify a specific population of patients with rheumatic diseases receiving rituximab treatment for whom the benefit from primary prophylaxis against Pneumocystis jirovecii pneumonia (PJP) outweighs the risk of adverse events (AEs).MethodsThis study included 818 patients treated with rituximab for rheumatic diseases, among whom 419 received prophylactic trimethoprim/sulfamethoxazole (TMP/SMX) with rituximab, while the remainder did not. Differences in 1‐year PJP incidence between the groups were estimated using Cox proportional hazards regression. Risk–benefit assessment was performed in subgroups stratified according to risk factors based on the number needed to treat (NNT) to prevent 1 case of PJP and the number needed to harm (NNH) due to severe AEs. Inverse probability of treatment weighting was applied to minimize the confounding by indication.ResultsDuring the 663.1 person‐years, there were 11 PJP cases, with a mortality rate of 63.6%. Concomitant use of high‐dose glucocorticoids (≥30 mg/day of prednisone or equivalent during 4 weeks after rituximab administration) was the most important risk factor. The PJP incidence (per 100 person‐years) was 7.93 (95% confidence interval [95% CI] 2.91–17.25) in the subgroup receiving high‐dose glucocorticoids compared with 0.40 (95% CI 0.01–2.25) in the subgroup without high‐dose glucocorticoid use. Although prophylactic TMP/SMX significantly reduced the overall PJP incidence (HR 0.11 [95% CI 0.03–0.43]), the NNT to prevent 1 case of PJP (146) was higher than the NNH (86). In contrast, the NNT fell to 20 (95% CI 10.7–65.7) in patients receiving concomitant high‐dose glucocorticoids.ConclusionThe benefit associated with primary PJP prophylaxis outweighs the risk of severe AEs in patients with rheumatic diseases receiving rituximab and concomitant high‐dose glucocorticoid treatment.
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A Review on Low‐Molecular‐Weight Gels Driven by Halogen‐Effect
Authors: Zhang Yuping , Wang Jing , Liao Yonggui , Xie Xiaolin 
Published: April 2023

AbstractAs a new type of non‐covalent interaction similar to hydrogen bond, halogen bond has become an important supramolecular tool in crystal engineering, material chemistry, biological science, etc., due to its unique properties. In fact, halogen bond has been confirmed on the effect of molecular assemblies and soft materials, and widely used in various functional soft materials including liquid crystals, gels and polymers. In recent years, halogen bonding has aroused strong interest in inducing molecular assembly into low‐molecular‐weight gels (LMWGs). To the best of our knowledge, there is still a lack of in‐depth review of this field. So, in this paper, the recent progress of LMWGs driven by halogen bonding is reviewed. According to the number of components forming halogen bonded gels, the structural characteristics of halogen bonded supramolecular gels, the relationship between halogen bonding and other non‐covalent interactions, as well as the application fields of halogen bonded gels are introduced, respectively. In addition, the challenges faced by halogenated supramolecular gels at present and their development prospects in future have been proposed. We believe that the halogen bonded gel will have more impressive applications in the next few years, opening exciting new opportunities for the development of soft materials.
Expression, purification, and biological characterization of recombinant human interleukin‐31 protein
Authors: Chen Jing , Zheng Yuxin , Wang Lixian , Pang Xuefei , Gao Feng , Xiao Haixia , Huo Nairui 
Published: April 2023

AbstractInterleukin‐31 (IL‐31), belonging to the IL‐6 cytokine family, is involved in skin inflammation and pruritus, as well as some tumors’ progression. Here, we reported the expression and purification of recombinant human IL‐31 (rhIL‐31) using a prokaryotic system. This recombinant protein was expressed in the form of inclusion bodies, refolded and purified by size‐exclusion chromatography. Circular dichroism analysis revealed that the secondary structure of rhIL‐31 was mainly composed of alpha‐helix, which is in consistence with the 3D model structure built by AlphaFold server. In vitro studies showed that rhIL‐31 exhibited a good binding ability to the recombinant hIL‐31 receptor alpha fused with human Fc fragment (rhIL‐31RA‐hFc) with EC50 value of 16.36 µg/mL in ELISA assay. Meanwhile, flow cytometry demonstrated that rhIL‐31 was able to bind to hIL‐31RA or hOSMRβ expressed on the cell surface, independently. Furthermore, rhIL‐31 could induce the phosphorylation of STAT3 in A549 cells. In conclusion, the prepared rhIL‐31 in this study possesses the binding ability to its receptors, and can activate the signal pathway of JAK/STAT. Thus, it can be applied in further studies, including investigation of hIL‐31‐related diseases, structural analysis, and development of therapeutic drugs, and monoclonal antibodies targeting hIL‐31.
An Ether‐Based Low Density Electrolyte for the Use of Graphite Anodes in Stable Lithium‐Sulfur Batteries
Authors: Hoffmann Florian S. , Schmidt Florian , Müller Jannes , Schönherr Kay , Dörfler Susanne , Abendroth Thomas , Althues Holger , Kwade Arno , Wu Nae‐Lih , Kaskel Stefan 
Published: April 2023

AbstractLithium sulfur (Li−S) batteries represent an interesting technology due to the high theoretical capacity of sulfur and the low cost of the cathode material. Li−S cells with graphite electrodes could be an option for low‐cost stationary energy storage as graphite is cheap and the electrode production process is well established. Unfortunately, in most Li−S electrolytes, graphite is not stable due to solvent co‐intercalation and degrades fast. In this work, a new low density electrolyte based on hexyl methyl ether (HME) and 1,3‐dioxalane (DOL) is presented, which allows to use graphite as anode material for Li−S batteries. In symmetric graphite vs. graphite cells an averaged Coulombic efficiency of 99.94 % per electrode could be reached. For the first time, cycling conditions like voltage window and balancing were optimized for Li−S cells with graphite anodes and the suitability of the concept could be demonstrated in multilayer pouch cells under realistic conditions.
An anatomical study of the suboccipital cavernous sinus and its relationship with the myodural bridge complex
Authors: Zhang Xu‐Hui , Gong Jin , Song Yang , Hack Gary D. , Jiang Si‐Meng , Yu Sheng‐Bo , Song Xue , Zhang Jing , Yang Heng , Cheng Jing , Sui Hong‐Jin , Zheng Nan 
Published: April 2023

AbstractThe suboccipital cavernous sinus (SCS) and the myodural bridge complex (MDBC) are both located in the suboccipital region. The SCS is regarded as a route for venous intracranial outflow and is often encountered during surgery. The MDBC consists of the suboccipital muscles, nuchal ligament, and myodural bridge and could be a power source for cerebrospinal fluid circulation. Intracranial pressure depends on intracranial blood volume and the cerebrospinal fluid. Since the SCS and MDBC have similar anatomical locations and functions, the aim of the present study was to reveal the relationships between them and the detailed anatomical characteristics of the SCS. The study involved gross dissection, histological staining, P45 plastination, and three‐dimensional visualization techniques. The SCS consists of many small venous sinuses enclosed within a thin fibrous membrane that is strengthened by a fibrous arch closing the vertebral artery groove. The venous vessels are more abundant in the lateral and medial portions of the SCS than the middle portion. The middle and medial portions of the SCS are covered by the MDBC. Type I collagen fibers arranged in parallel and originating from the MDBC terminate on the SCS either directly or indirectly via the fibrous arch. The morphological features of SCS revealed in this research could serve as an anatomical basis for upper neck surgical procedures. There are parallel arrangements of type I collagen fibers between the MDBC and the SCS. The MDBC could change the blood volume in the SCS by pulling its wall during the head movement.
Pilot study of implementing the Shared Healthcare Actions & Reflections Electronic systems in Survivorship (SHARE‐S) program in coordination with clinical care
Authors: Sohl Stephanie J. , Sadasivam Rajani S. , Kittel Carol , Dressler Emily V. , Wentworth Stacy , Balakrishnan Kavitha , Weaver Kathryn E. , Dellinger Rebecca Ann , Puccinelli‐Ortega Nicole , Cutrona Sarah L. , Foley Kristie L. , Houston Thomas 
Published: April 2023

AbstractIntroductionInitial cancer survivorship care planning efforts focused on information sharing demonstrated limited impact on patient health outcomes. We designed the Shared Healthcare Actions & Reflections Electronic Systems in survivorship (SHARE‐S) program to enhance survivorship guideline implementation by transitioning some effort from clinicians to technology and patients through supporting health self‐management (e.g., healthy lifestyles).MethodsWe conducted a single‐group hybrid implementation‐effectiveness pilot study. SHARE‐S incorporated three strategies: (1) e‐referral from the clinical team for patient engagement, (2) three health self‐management coach calls, and (3) text messages to enhance coaching. Our primary implementation measure was the proportion of patients e‐referred who enrolled (target >30%). Secondary implementation measures assessed patient engagement. We also measured effectiveness by describing changes in patient health outcomes.ResultsOf the 118 cancer survivor patients e‐referred, 40 engaged in SHARE‐S (proportion enrolled = 34%). Participants had a mean age of 57.4 years (SD = 15.7), 73% were female, 23% were Black/African American, and 5 (12.5%) were from a rural location. Patient‐level adherence to coach calls was >90%. Changes from baseline to follow‐up showed at least a small effect (Cohen's d = 0.2) for improvements in: mindful attention, alcohol use, physical activity, fruit and vegetable intake, days of mindfulness practice, depressive symptoms, ability to participate in social roles and activities, cancer‐specific quality of life, benefits of having cancer, and positive feelings.ConclusionThe SHARE‐S program successfully engaged cancer survivor patients. Once enrolled, patients showed promising improvements in health outcomes. Supporting patient self‐management is an important component of optimizing delivery of cancer survivorship care.
Phosphatidylcholine in bile‐derived small extracellular vesicles as a novel biomarker of cholangiocarcinoma
Authors: Muraki Ryuta , Morita Yoshifumi , Ida Shinya , Kitajima Ryo , Furuhashi Satoru , Takeda Makoto , Kikuchi Hirotoshi , Hiramatsu Yoshihiro , Takanashi Yusuke , Hamaya Yasushi , Sugimoto Ken , Ito Jun , Kawata Kazuhito , Kawasaki Hideya , Sato Tomohito , Kahyo Tomoaki , Setou Mitsutoshi , Takeuchi Hiroya 
Published: April 2023

AbstractBackgroundOwing to the lack of definite diagnostic modalities, it is challenging to distinguish malignant cases of cholangiocarcinoma (CCA), which often causes biliary tract obstruction, from benign ones. Here, we investigated a novel lipid biomarker of CCA in bile‐derived small extracellular vesicles (sEVs) and developed a simple detection method for clinical application.MethodsBile samples from seven patients with malignant diseases (hilar CCA = 4, distal CCA = 3) and eight patients with benign diseases (gallstones = 6, primary sclerosing cholangitis = 1, autoimmune pancreatitis = 1) were collected through a nasal biliary drainage tube. sEVs were isolated via serial ultracentrifugation and characterized using nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting (with CD9, CD63, CD81, and TSG101). Comprehensive lipidomic analysis was performed using liquid chromatography–tandem mass spectrometry. Using a measurement kit, we further confirmed whether lipid concentrations could be used as a potential CCA marker.ResultsLipidomic analysis of bile sEVs in the two groups identified 209 significantly increased lipid species in the malignant group. When focusing on lipid class, phosphatidylcholine (PC) level was 4.98‐fold higher in the malignant group than in the benign group (P = 0.037). The receiver operating characteristic (ROC) curve showed a sensitivity of 71.4%, a specificity of 100%, and an area under the curve (AUC) of 0.857 (95% confidence interval [CI]:0.643–1.000). Using a PC assay kit, the ROC curve showed a cutoff value of 16.1 μg/mL, a sensitivity of 71.4%, a specificity of 100%, and an AUC of 0.839 (95% CI: 0.620–1.000).ConclusionPC level in sEVs from human bile is a potential diagnostic marker for CCA and can be assessed by a commercially available assay kit.
Analysis of the efficacy and influencing factors of preoperative P‐SOX neoadjuvant chemotherapy regimen for progressive gastric cancer—construction of a clinical prediction model
Authors: Feng Long , Shao Lei , Sun Shuangshuang , Zhang Chengwu , Cai Baojia 
Published: April 2023

AbstractPreoperative neoadjuvant chemotherapy is one of the most common treatments for patients with advanced gastric cancer that cannot be completely removed by surgery. Nab‐paclitaxel is a nano‐formulation of paclitaxel that has been shown to be effective in treating stomach cancer. In addition, oxaliplatin + S‐1 (SOX) has been a first‐line chemotherapy regimen for gastric cancer, and it has the effect of tumor downstaging, improving the R0 resection rate, and reducing the postoperative recurrence rate, but the side effects are significant. During the application of oxaliplatin, obvious gastrointestinal reactions such as nausea and vomiting can be observed. There may also be blood system side effects such as leukopenia and thrombocytopenia, as well as serious adverse reactions such as peripheral neuropathy. Therefore, we reduced the amount of oxaliplatin in SOX and added nab‐paclitaxel on the basis of this, in order to increase the efficacy while reducing the side effects of SOX regimen. We selected 192 patients with advanced gastric cancer admitted to the Department of Gastrointestinal Oncology of Qinghai University Hospital from July 2019 to February 2022, and all were treated with nab‐paclitaxel plus oxaliplatin + S‐1 neoadjuvant chemotherapy regimen, and underwent further surgery after chemotherapy. The tumor regression grade (TRG grade) and response evaluation criteria of solid tumor 1.1 (RECIST1.1) were taken as the dependent variables. According to TRG classification, 120 patients were effective (grade 0, 1, 2 = 62.50%, age: 55.63 ± 9.02 years), 72 patients were ineffective (grade 3 = 37.50%, 55.82 ± 9.21 years), and the effective rate of chemotherapy was 62.50%. According to RECIST1.1, 116 patients were effective (CR + PR = 60.42%, mean age 55.84 ± 9.02 years), 76 patients were ineffective (SD + PD = 39.58%, 55.47 ± 9.19 years), and the effective rate was 60.42%. The factors p < 0.2 in univariate logistic regression analysis were included in multivariate logistic regression analysis, and p < 0.05 was the statistical difference, and statistically significant factors were screened out for modeling and plotted the nomogram. Among them, in the tumor regression grade, the final factors related to effective chemotherapy are the degree of differentiation, cT. stage, tumor diameter, chemotherapy cycle, and the final factors related to effective chemotherapy in the solid tumor response evaluation criteria are the degree of differentiation, cT. stage, tumor diameter. Therefore, we conclude that the regimen of nab‐paclitaxel combined with oxaliplatin and S‐1 has certain positive significance in the treatment of advanced gastric cancer.
High‐efficiency sodium storage of Co0.85Se/WSe2 encapsulated in N‐doped carbon polyhedron via vacancy and heterojunction engineering
Authors: Pei Ya Ru , Zhou Hong Yu , Zhao Ming , Li Jian Chen , Ge Xin , Zhang Wei , Yang Chun Cheng , Jiang Qing 
Published: April 2023

AbstractWith the advantage of fast charge transfer, heterojunction engineering is identified as a viable method to reinforce the anodes' sodium storage performance. Also, vacancies can effectively strengthen the Na+ adsorption ability and provide extra active sites for Na+ adsorption. However, their synchronous engineering is rarely reported. Herein, a hybrid of Co0.85Se/WSe2 heterostructure with Se vacancies and N‐doped carbon polyhedron (CoWSe/NCP) has been fabricated for the first time via a hydrothermal and subsequent selenization strategy. Spherical aberration‐corrected transmission electron microscopy confirms the phase interface of the Co0.85Se/WSe2 heterostructure and the existence of Se vacancies. Density functional theory simulations reveal the accelerated charge transfer and enhanced Na+ adsorption ability, which are contributed by the Co0.85Se/WSe2 heterostructure and Se vacancies, respectively. As expected, the CoWSe/NCP anode in sodium‐ion battery achieves outstanding rate capability (339.6 mAh g−1 at 20 A g−1), outperforming almost all Co/W‐based selenides.
Helical Anatase Titanium Nanotubes through a Protected Crystallization Strategy for Enhanced Photocatalytic Performance
Authors: Liu Xinrong , Shi Mengke , Tang Xiaoyu , Ma Yue , Dang Jingshuang , Yan Xumei , Gu Quan , Bao Zijia , Liang Zuozhong , Zhang Wei , Cao Rui , Zheng Haoquan 
Published: April 2023

AbstractHelical structure in catalysts has attracted attention and been recently investigated for various catalytic reactions. However, helical transition metal oxides suffer from uncontrollable crystallization processes at high temperatures when being transformed from an amorphous phase into a crystalline structure. Herein, we report a helical anatase TiO2 nanotube for the first time, which has been prepared using a protected crystallization strategy in the confined space of silica. A single chirality of helical TiO2 has been used to track the ordering of the twisted structure. The twisted structure in helical anatase TiO2 nanotube is maintained after a vigorous crystallization process. Helical anatase TiO2 nanotubes possess more accessible active sites and abundant defects of oxygen vacancy and Ti3+ species owing to the twisted structure. The obtained helical anatase TiO2 nanotube exhibits superior photocatalytic activity for hydrogen production without adding any co‐catalysts. This work provides new insights into the role of helical structure in transition metal‐based catalysts.