bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between vitamin D and antimicrobial peptide LL-37 in children with respiratory syncytial virus pneumonia
Authors: He Yixiu , Zhao Shaojie , Pan Menglan , Chen Bingzhang , Zhang Yufeng , Zhu Yafei 
Published: March 2023

Abstract

Objective
We investigated the effects of serum vitamin D and antimicrobial peptide LL-37 levels on the respiratory syncytial virus (RSV) pneumonia in children.
Methods
From January 2021 to December 2021, 81 children with RSV pneumonia (case group) were divided into severe group (n = 29) and normal group (n = 52) according to the 2019 edition of the expert consensus on childhood pneumonia, while 45 health clinic checkup children were selected as controls. Serum 25-(OH)D levels were measured by electrochemiluminescence and serum LL-37 levels were measured by enzyme-linked immunosorbent assay. Levels and correlations between groups were then statistically analyzed.
Results
25-(OH)D levels in the case group (91.25 ± 24.80 nmol/L) were significantly lower than in the healthy controls (104.91 ± 22.23 nmol/L, P < 0.01). LL-37 levels in the case group (323.81 ± 91.51 pg/mL) were higher than levels in the healthy controls (260.85 ± 38.48 pg/mL, P < 0.001). We identified a correlation between 25-(OH)D and LL-37 levels in the case group (r = 0.231, P = 0.04). We observed no significant differences in 25-(OH)D and LL-37 levels between affected children in winter and spring periods, and affected children in summer and autumn periods (P > 0.05). When common group compared with the severe group of RSV pneumonia, there were significant differences in LL-37 levels (t = -3.611, P = 0.001), white blood cell (WBC) ≥ 10×109/L (χ2 = 7.89, P < 0.01) and C-reaction protein (CRP) < 10 mg/L (χ2 = 4.71, P = 0.03) and there was no significant difference in the 25-(OH)D levels (t = -1.08, P > 0.05). Receiver-operating characteristic (ROC) curve analyses showed that the optimal critical serum LL-37 value was 384.24 pg/mL, and corresponding specificity and sensitivity values were 90.4% and 58.6%, respectively. Severe RSV pneumonia risk of LL-37 ≥ 384.24 pg/mL was significantly higher than that of the LL-37 < 384.24 pg/mL group (χ2 = 22.60, P < 0.001). Using multivariate logistic regression analysis, serum LL-37 ≥ 384.24 pg/mL (odds ratio (OR) = 17.127, P < 0.001), WBC ≥ 10×109/L (OR = 7.399, P < 0.01), and CRP < 10 mg/L (OR = 12.393, P = 0.02) may be risk factors for severe RSV pneumonia in children.
Conclusions
25-(OH)D and LL-37 levels are involved in RSV pneumonia, with little seasonal correlations. High LL-37, WBC ≥ 10×109/L, and CRP < 10 mg/L levels are probably related to disease severity and may be used for disease evaluation.
A Case Series of Acute Symptomatic Hyponatremia due to SIADH in mild COVID-19 Infection
Authors: Devaraja Vanusha , Foo Siew Hui , Muniandy Jothinathan 
Published: March 2023

Abstract
Syndrome of Inappropriate anti diuretic hormone (SIADH) is one of the commonest cause of hyponatremia among medical inpatients. Over recent years, the evolution of SARS-COV-2 infection has led to atypical presentations of acute symptomatic hyponatremia secondary to isolated SIADH exclusive of pneumonia. We report an unusual case series of acute symptomatic hyponatremia secondary to SIADH in Category 2 COVID-19 infection. In our case series, all the patients presented with symptoms of acute severe hyponatremia and were incidentally screened positive for the SARS-COV-2 virus without respiratory tract symptoms and normal chest imaging. They were fully vaccinated and boosted at least three months before the presentation. Clinical and biochemical workups confirmed SIADH in all three patients. They were treated with hypertonic saline initially, followed by fluid restriction as per recommendations. It was postulated that the most likely mechanism responsible for the inappropriate ADH secretion is mediated by the increased inflammatory cytokines, especially interleukin-6 and the direct effect of the SARS-COV2 infection. In the context of the COVID-19 pandemic, atypical presentations of acute symptomatic hyponatremia without an apparent cause could be an isolated manifestation of SARS-COV-2 infection. Awareness of this condition is essential for the early institution of the treatment protocol for this reversible and life-threatening disorder
Flood insurance systems drive population growth in floodplains
Authors: Tesselaar Max , Botzen W.J. Wouter , Tiggeloven Timothy , Aerts Jeroen 
Published: March 2023

Abstract
A substantial literature about future flood risk assessment focuses on changing hazard conditions as a result of climate change, where flood exposure is assumed to remain static or develop according to exogenous scenarios. This study presents an innovative method to project future flood risk by simulating population growth in floodplains, where settlement location decisions of households endogenously depend on environmental and institutional factors, including amenities associated with river proximity, flood risk, and insurance against this risk. Our results show that population growth in floodplains, and consequently rising flood risk, are considerably higher when the disamenity caused by flood risk is offset by insurance. This outcome is particularly evident in countries where flood risk is covered collectively, and notably less where premiums reflect the risk of individual households.
Construction of a strawberry breeding core collection to capture and exploit genetic variation
Authors: Koorevaar Tim , Willemsen Johan H , Visser Richard GF , Arens Paul , Maliepaard Chris 
Published: March 2023

Abstract
Genetic diversity is crucial for the success of plant breeding programs and core collections that capture this diversity are an important resource to exploit this. In this study, we present a method for constructing a core collection that integrates both genomic and pedigree information to capture maximum genetic variation in a minimum subset of genotypes within a strawberry breeding program, while also being future proof. Our stepwise approach starts with selecting the most important crossing parents of advanced selections and genotypes included for specific traits, to represent also future genetic variation. We then use pedigree-genomic-based relationship coefficients combined with the ‘accession to nearest entry’ criterion to complement the core collection and maximize its representativeness of the current breeding program. Combined pedigree-genomic-based relationship coefficients allow for accurate relationship estimation without the need to genotype every individual in the breeding program. The resulting core collection can for example be used to develop a haplotype reference panel for imputation-based genotyping methods.
Assessing the Impact of Nutrition Education on Antenatal Nutrition Knowledge of Women in Lebanon: an Interventional Study
Authors: Chehade Christina Al Hajj , Yeretzian Joumana Estephan , Dano Dominique 
Published: March 2023

Abstract
Background
Nutrition education during pregnancy has significant impact on improving knowledge and dietary habits of pregnant women, which in turn leads to enhanced postpartum maternal and birth outcomes. The aim of this study is to evaluate the impact of antenatal nutrition education on the knowledge of women of childbearing age in Lebanon.
Method
A quantitative quasi-experimental study design was adopted. Online antenatal nutrition education sessions were provided to a group of women of childbearing age. The online session was ninety minutes long and covered antenatal weight gain and its composition, nutrition in the preconception and the antenatal periods, food safety, and nutrition intervention for nutrition related pregnancy complications. Participants’ antenatal nutrition knowledge was assessed before and directly after the session through an online self-administered questionnaire. Descriptive and inferential statistics were employed to analyze the results, using SPSS 28®.
Results
A total of 5 virtual sessions on antenatal nutrition were given by a licensed dietitian and attended by 91 participants. Fifty nine percent of participants had previously received antenatal nutrition information, of which 27.5% were obtained from dietitians and 22% were obtained from the internet. Mean scores for pretest and posttest were 27 ± 6.5 and 37 ± 4.1 respectively, and their difference was statistically significant (t=-31.6; P < 0.001). In the pretest, the lowest score was 6 and the maximum score was 39 ± 6.5. However, the lowest score in the posttest was 21 and the highest was 41 ± 4.1. The statement related to the recommended amount of caffeine consumption among pregnant women received the lowest percentage of correct answers (3%). However, in the posttest, this number improved and increased to 85% correct answers. On the other hand, 99% of answers were correct regarding nutrition affecting pregnancy outcomes, making it the highest percentage of correct answers at pretest.
Conclusion
Antenatal nutrition education may improve knowledge and practice of women during pregnancy. Thus, attention should be directed towards promoting nutrition education of women of childbearing age.
Predictive Factors for Early Neurological Deterioration after intravenous thrombolysis of Single Small Subcortical Infarction in the Territory of the Middle Cerebral Artery
Authors: Zhao Meng , Zhong Xuemin , Du Jiaxiu , He Lanying , Wang Jian 
Published: March 2023

Abstract
Background
Patients with single subcortical infarction (SSI) in the middle cerebral artery (MCA) will often suffer from early neurological deterioration (END) despite receiving intravenous thrombolytic therapy (IVT). In this study, the predictors for END were studied in patients with single subcortical infarction in the MCA after IVT.
Methods
Patients with SSIs in the territory of the middle cerebral artery with IVT between June 2020 and June 2022 were included in this study. END was defined as an ≥ 2 increase in the total National Institutes of Health Stroke Scale (NIHSS) score or ≥ 1 in the motor NIHSS score within the first 72 hours of admission. In addition to the analysis of the predictors of END in SSI patients who received IVT in the territory of MCA, proximal and distal SSI patients were also analyzed.
Results
A total of 174 patients with SSI in the territory of the MCA who received IVT were evaluated. In multivariable logistic regression analysis, the presence of pSSI (odds ratio, 5.98;95% CI, 2.13–16.78; P = 0.001), lower TT (odds ratio, 0.694; 95% CI, 0.511 ~ 0.943; P = 0.019), and higher red blood cells (odds ratio,2.047; 95% CI, 1.120 ~ 3.741; P = 0.020) were positively associated with END in patients with proximal SSI (pSSI). In patients with pSSI, there was a positive correlation between AST and blood sugar. Meanwhile, no variable related to END was found in the distal SSI (dSSI) group.
Conclusions
The presence of pSSI, lower TT, and higher red blood cells are associated with END in patients with SSI in the territory of the MCA who received IVT. The frequency and predictors of SSI in the territory of the MCA were different between proximal and distal SSI.
An unified approach for the calculation of different sample-based measures with single sampling method
Authors: Leszczyński Maciej , Perlikowski Przemyslaw , Brzeski Piotr 
Published: March 2023

Abstract
This paper investigates two sample-based methods for analysing multistable systems, i.e., basin stability and basin entropy. Both methods are based on the large number of numerical integration trials obtained for a large set of different initial conditions. The obtained data is classified and used to determine measures that describe the stability of solutions, the structure of the phase space and the predictability of system dynamics. The basin stability reflects the overall probability of reaching given solutions, and the basin entropy measure was proposed to reflect the structure of basins of attraction and the complexity of their boundaries. While these two measures complement one another finely, the procedures originally proposed to obtain them differ significantly. The paper proposes a universal approach and algorithm to obtain the value of basin stability and basin entropy measures. The applicability of the proposed procedures is presented for two nonlinear systems.
An Intelligent Cloud Robotics Personalized Medicine System by Assessment of DNA and Electronic Health Records Using Fuzzy Based Progressive Alignment Sequence (F-PAS)
Authors: Jayanthi Appawala , Reddy B Eswara 
Published: March 2023

Abstract
Treatment and prevention in the standard approach are focused on statistical averages rather than the unique needs of each individual. The one-size-fits-all strategy is one of the conventional classification methods for treating and preventing people. Potentially replacing conventional trial-and-error therapy, personalized medicine could provide patient care with the best medications and the highest levels of safety. This strategy allows physicians to prognosticate the disease with high precision and suggest therapy and prevention approaches based on the patient’s health, personal information, and related groups. With the growth of artificial intelligence, several humanistic intelligent methods are intended to give services with better quality, like intelligent healthcare, emotional communication, and innovative systems. Cognitive cloud computing is the essential technology to drive above intelligent robotic system. In this paper, we introduced a framework to analyze the patient’s body nature using his DNA and Electronic Health Records (EHRs) and suggested the right drug at the right time with the proper dosage. Here, Cognitive computing plays a vital role in analyzing the DNA sequence and EHRs with a huge heterogeneous database. It enables the patient to receive earlier diagnoses, risk assessments, and optimal treatments, improving health care at a low cost. The F-PAS (fuzzy based progressive alignment sequence approach) is proposed for effective assessment of DNA and EHRs. The proposed system reduces the cost of computing for comparing all of the patients in the database by around 60%. The outcomes of the experiments demonstrate that the proposed framework minimizes computational costs and aids physicians in recommending the appropriate medication at the appropriate time and dosage.
The tannic acid-iron dynamic redox self-catalytic system mediated way to simple and rapidly fabricate a multifunctionally conductive hydrogel for wearable strain sensors
Authors: Miao Gan , Li Fangchao , Miao Xiao , Hou Zhiqiang , Xu Lide , Xu Ting , Ren Guina , Yang Xiaoyang , Zhu Xiaotao 
Published: March 2023

Abstract
Conductive hydrogels have gained increasing attention in the field of wearable smart devices. However, it remains a big challenge to develop multifunctionally conductive hydrogel in a rapid and facile way. Herein, a conductive tannic acid-iron/poly (acrylic acid) hydrogel was synthesized within 30 s at ambient temperature by the tannic acid-iron (TA@Fe3+) mediated dynamic catalytic system. The TA@Fe3+ dynamic redox autocatalytic pair could efficiently activate the ammonium persulfate to initiate the free-radical polymerization, allowing the gelation to occur easily and rapidly. The resulting hydrogel exhibited enhanced stretchability (300%), conductivity (33.58 S/m), and strain sensitivity (gauge factor = 2.11). When damaged, it could be self-healed through the dynamic and reversible coordination bonds between the Fe3+ and COO- groups in the hydrogel network. Interestingly, the resulting hydrogel could act as a strain sensor to monitor various human motions including the huge movement of deformations (knuckle, wrist) and subtle motions (smiling, breathing) in real-time, due to its enhanced self-adhesion, good conductivity, and improved strain sensitivity. Also, the obtained hydrogel exhibited efficient electromagnetic interference (EMI) shielding performance with an EMI shielding effectiveness value of 24.5 dB in the X-band (8.2-12.4 GHz). Additionally, it displayed antibacterial properties, with the help of the activity of TA.
CENPA facilitates hepatocellular carcinoma proliferation by cooperating with YY1 to drive transcription of CCND1 and NRP2.
Authors: Liao Jingyu , Chen Zeyu , Chang Ruizhi , Yuan Tong , Li Ganxun , Zhu Chang , Wen Jingyuan , Wei Yi , Huang Zhao , Ding Zeyang , Liang Junnan , Chu Liang , Zhang Bixiang 
Published: March 2023

Abstract
Background
The centromere proteins (CENPs) are the key regulators in mitosis-related protein complexes and involved in kinetochore assembly and chromosome segregation during mitosis, yet they remain largely unexplored in hepatocellular carcinoma (HCC).
Methods
In our study, we analyzed the potential role of CENPs family numbers by bioinformatic analysis. We detected the expression level of Centromere protein A (CENPA) in HCC tissues and cells by qRT-PCR, IHC and western blotting. Furthermore, CCK-8, colony formation, EdU and flow cytometry assays were used to evaluated the potential function of CENPA in HCC cells. Subcutaneous and orthotopically mouse model were used to explored the oncogenic role of CENPA. Besides, we demonstrated the interaction between CENPA and YY1 by silver staining and co-immunoprecipitation (co-IP) assays. Dual luciferase reporter and ChIP-sequencing (ChIP-seq) assays were used to identify the potential binding sites of CENPA and YY1 on CCND1 and NRP2 promoter regions.
Results
In this study, CENPA was identified significantly up-regulated in HCC, and the CENPA overexpression was linked to the poor prognosis of HCC patients. CENPA knockdown inhibited the HCC cell proliferation and tumor growth. Mechanistically, CENPA activated YY1 transcription and cooperatively interacted with YY1 to bind at cyclin D1 (CCND1) and neuropilin 2 (NRP2) promoter regions for transcriptional activation. Moreover, we identified a lactylation modification site (K124) of CENPA, which is essential for CENPA to activate its target genes transcriptionally.
Conclusions
These findings indicate the synergistic mechanism between CENPA and YY1 that promotes CCND1 and NRP2 expression, which leads to HCC progression.
A numerical framework for linear stability analysis of two-phase stratified pipe flows
Authors: Barmak Ilya , Gelfgat Alexander , Brauner Neima 
Published: March 2023

Abstract
A numerical framework for rigorous linear stability analysis of two-phase stratified flows of two immiscible fluids in horizontal circular pipes is presented. For the first time, three-dimensional disturbances, including those at the interface between two fluids, are considered. The proposed numerical framework is based on a finite-volume method and allows solving the problem numerically in bipolar cylindrical coordinates. In these coordinates, both the pipe wall and the unperturbed interface (of a constant curvature, e.g., plane interface, as considered in this work) coincide with the coordinate surfaces. Thereby, the no-slip as well as the interfacial boundary conditions can be imposed easily. It also enables investigation of the local behavior of the flow field and shear stresses in the vicinity of the triple points, where the interface contacts the pipe wall. The results obtained in the bipolar coordinates are verified by an independent numerical solution based on the problem formulation in Cartesian coordinates, where the pipe wall is treated by the immersed boundary method. Two representative examples of gas-liquid and liquid-liquid flows are included to demonstrate the applicability of the proposed numerical technique for analyzing the flow stability.
Shotgun metagenomics reveals interkingdom association between intestinal bacteria and fungi involving competition for nutrients
Authors: Xie Zixuan , Canalda-Baltrons Aleix , d'Enfert Christophe , Manichanh Chaysavanh 
Published: March 2023

Abstract
Background: The accuracy of internal-transcribed-spacer (ITS) and shotgun metagenomics has not been robustly evaluated, and the effect of diet on the composition and function of the bacterial and fungal gut microbiome in a longitudinal setting has been poorly investigated. Here we compared two approaches to study the fungal community (ITS and shotgun metagenomics), proposed an enrichment protocol to perform a reliable mycobiome analysis using a comprehensive in-house fungal database, and correlated dietary data with both bacterial and fungal communities.
Results: We found that shotgun DNA sequencing after a new enrichment protocol combined with the most comprehensive and novel fungal databases provided a cost-effective approach to perform gut mycobiome profiling at the species level and to integrate bacterial and fungal community analyses in fecal samples. The mycobiome was significantly more dynamic than the bacterial community at the compositional and functional levels. Notably, we showed that microbial diversity, composition, and functions were associated with habitual diet composition instead of driven by global dietary changes. Our study highlights a competitive inter-kingdom interaction between bacteria and fungi for food foraging.
Conclusion: Together, our present work proposes an efficient workflow to study the human gut microbiome integrating robustly fungal, bacterial, and dietary data. These findings will further advance our knowledge of the interaction between gut bacteria and fungi and pave the way for future investigations in human mycobiome.
The Effect of the Use of a Walker in Infancy on the Musculoskeletal System and the Level of Physical Activity
Authors: Gokcek Ozden , Tugral Yagmur , Denız Mıne Argalı , Huzmelı Esra Doğru , Ozkeskin Mehmet , Baser Mıray , Hurrıyetoglu Arzu , Yıldırım Baran , El Cigdem 
Published: March 2023

Abstract
Background
The study aimed to compare the lower extremity muscle strength, postural disorders, and physical activity levels of children who used and did not use a walker during infancy.
Method
The study included 39 children between the ages of 8 and 15. The demographic information of the children, their motor development stages and walker use situations, the age at which they started to use it, and the duration of use was questioned. The New York Posture Analysis Questionnaire and the Physical Activity Assessment Questionnaire Scale (PAQ-C) were given to the children. Muscle strength was measured with a digital muscle strength meter.
Results
For the left hip abductor, right hip adductor, right foot plantar flexor, both lower extremity dorsiflexor muscles, and PAQ-C weekend item parameters, a statistically significant difference was found between the two groups (p˂0.05).
Conclusion
We believe that future age periods should be followed up to examine the effect of the use of walkers in children in the developmental period, and the predicted possible effects may yield findings, especially at later ages. The lack of statistical differences in posture and muscle strength necessitated the association of childhood with adulthood.