bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircBAGE2 (hsa_circ_0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p ‘sponge’ to alter the proliferation and apoptosis of prostate cancer cells*
Authors: Zhang Chunlei , Liu Dan , Tian Qinqin , Yang Qi , Zi Xiaoyuan , Sun Yinghao 
Published: June 2023

Abstract

Objective
The aim of the article is to explore the function of circBAGE2 (hsa_circ_0061259) in prostate cancer (PCa) cells.

Methods
Sequencing results of circBAGE2 were verified by quantitative RT PCR (qRT-PCR) and Sanger sequencing. Agarose gel electrophoresis was used to detect the resistance of GAPDH, BAGE2, and circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells. The biological functions of circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs. Multiple databases were used to predict the target binding sites between circRNAs, miRNAs, and mRNAs. Western blotting was used to detect the expression of CCND1 and PDCD10.

Results
CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that in matched normal tissues and normal cells, and CircBAGE2 knockdown inhibits cell proliferation and promotes apoptosis. Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10. The 3′ UTRs of CCND1 and PDCD10 were matched by miR-103a-3p, which shared binding sites with circBAGE2.

Conclusion
CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression through its role as a ‘sponge’ of miR-103a-3p. CircBAGE2 may be a potential therapeutic target for PCa.
Differentially expressed genes analysis and target genes prediction of miR-22 in breast cancer*
Authors: Fan Tao , Wang Chaoqi , Zhang Kun , Yang Hong , Zhang Juan , Wu Wanyan , Song Yingjie 
Published: June 2023

Abstract

Objective
miR-22 is highly active in breast cancer, especially in the luminal B and HER2 subtypes. However, the detailed potential of the use of target genes for miR-22 in breast cancer are still unclear. In this study, we aimed to discover potential genes and the miRNA-DEGs network of miR-22 in breast cancer using bioinformatics approaches.

Methods
Analysis of microarray data GSE17508 (including 3 miR-22 knockout samples and 3 controls) obtained from the Gene Expression Omnibus (GEO) database was performed. Differentially expressed genes (DEGs) between the miR-22 knockout samples and the three control samples were detected using GEO2R. The gene ontology (GO) functional enrichment analysis and protein-protein interaction (PPI) network of DEGs were performed using the online tool Metascape and STRING database, separately. The miR-22 and DEG networks were obtained from the miRNet database. Cytoscape software was used to construct and analyze a merged miRNA-DEG network. The online tools database, mirDIP 4.1, was used to predict miR-22 target genes.

Results
Certain DEGs and miRNAs may be potential targets for predicting and treating miR-22 expressed breast cancer.

Conclusion
We constructed a prognostic model of rectal adenocarcinomas based on four immune-related lncRNAs by analyzing the data based on TCGA database, with high prediction accuracy. We also identified two biomarkers with poor prognosis (PXN-AS1 and AL158152.2) and one biomarker with good prognosis (LINC01871).
Application of endoscopic nasobiliary cutting in the treatment of hilar cholangiocarcinoma
Authors: Jiang Shujuan , Zhou Zhongyin 
Published: June 2023

Abstract

Objective
The aim of the study was to study the clinical efficacy and prognosis of endoscopically cutting the nasobiliary duct and leaving its residual segment as a biliary stent in the treatment of hilar cholangiocarcinoma (HC).

Methods
The clinical data of 55 patients with HC treated by endoscopic biliary drainage at the Gastrointestinal Endoscopy Center of our hospital (Renmin Hospital of Wuhan University, China) from August 2017 to August 2019 were retrospectively analyzed. According to different drainage schemes, patients were divided into the endoscopic nasobiliary cutting group (n = 26) and the endoscopic retrograde biliary drainage (ERBD) group (n = 29). The postoperative liver function indexes, incidence of postoperative complications, median patency period of stents, and median survival time of patients were compared between the two groups.

Results
Liver function indexes (total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transpeptidase) were significantly decreased in 55 patients a week postoperaticely (P < 0.05), and decreases in liver function indexes in the endoscopic nasobiliary cutting group were more significant than those in the ERBD group (P < 0.05). The incidence of biliary tract infection in the endoscopic nasobiliary cutting group was significantly lower than that in the ERBD group (15.40% vs. 41.4%, P < 0.05). In the endoscopic nasobiliary cutting subgroups, there were 1 and 3 cases of biliary tract infection in the gastric antrum cutting group (n = 21) and duodenal papilla cutting group (n = 5), respectively, and 0 cases and 2 cases of displacement, respectively; there was a statistically significant difference in terms of complications between the two subgroups (P < 0.05). The median patency period (190 days) and median survival time (230 days) in the nasobiliary duct cutting group were higher than those (169 days and 202 days) in the ERBD group, but there was no significant difference (P > 0.05).

Conclusion
The nasobiliary duct was cut by using endoscopic scissors in Stage II after the bile was fully drained through the nasobiliary duct. The residual segment could still support the bile duct and drain bile. The reduction of jaundice and the recovery of liver enzymes were significant, and the incidence of biliary tract infection was low. Cutting off the nasobiliary duct at the duodenal papilla results in a higher incidence of biliary tract infection, and the residual segment of the nasobiliary duct is more likely to be displaced. Endoscopic nasobiliary-cutting drainage is an effective, simple, and safe method to reduce jaundice in the palliative treatment of HC.
Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer*
Authors: Chen Xia , Zhang Xiaoming , Lu Xiangji , Ren Meng , Su Rina , Gao Weishi , Gao Yanwei 
Published: June 2023

Abstract

Objective
The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes (HSP70-PCs) on HER-3-overexpressing breast cancer.

Methods
In this study, we first studied the expression of HER-3 in breast cancer tissues and its relationship with patient characteristics. We then purified HSP70-PCs from primary breast cancer cells with different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic cells (DCs) and CD8+ T cells induced by these complexes. Third, recombinant human HSP70-HER-3 protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3-overexpressing breast cancer cells, and the resulting immunological response was examined.

Results
The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these treatments induce autogenous CD8+ T cell killing of HER-3-positive breast cancer cells.

Conclusion
These findings provide a new direction for HSP70-DC-based immunotherapy for patients with HER-3-overexpressing breast cancer.
Recombinant human vascular endostatin injection to synchronize craniospinal radiotherapy for the treatment of recurrent medulloblastoma in children: A retrospective clinical study*
Authors: Song Yang , Xiao He , Chen Chuan , Liang Ping , Ji Wenyuan , Geng Mingying 
Published: June 2023

AbstractObjectiveMedulloblastoma (MB) is the most common primary central nervous system malignancy in children. Nonetheless, there is no standard treatment for recurrent MB. The purpose of this study was to investigate the clinical value and toxicity of recombinant human endostatin injection (Endostar®) combined with craniospinal radiotherapy for the treatment of recurrent MB in children.MethodsThis study retrospectively analyzed 13 patients with recurrent MB aged 5-18 years. Endostar® 7.5 mg/m2/d was synchronized during craniospinal radiotherapy for 7 children with a portable micro uniform speed infusion pump. Endostar®was applied 3 days prior to the initiation of radiotherapy. The drug was in continuous use for 7 days. Similarly, the withdrawal of the drug took place over 7 days. This represented a cycle. During radiotherapy, the application was repeated until the end of radiotherapy (experimental group). In the other 6 cases, only craniospinal radiotherapy was used (control group).ResultsThe complete remission rate was 71.4% in the experimental group and 16.7% in the control group. The median progression-free survival (PFS) was 14 months (95% CI: 0.0-29.60) and 19 months (95% CI: 0.0-39.53) in the experimental and control groups, respectively. The median overall survival (OS) was 19 months (95% CI: 0.0-38.20) and 23 months (95% CI: 2.47-43.53) in the experimental and control groups, respectively. The most common adverse events included grade 1 thrombocytopenia (7.7%), grade 3 neutropenia (38.5%), and grade 1 anemia (30.8%).ConclusionEndostar®synchronizing craniospinal radiotherapy significantly improved the complete response rate of children with recurrent MB. It did not increase the side effects of radiation therapy. However, it did not improve the PFS or OS.
A case of sorafenib-induced severe thrombocytopenia during treatment of unresectable hepatocellular carcinoma
Authors: Quan Xiaoying , Chen Xiaoyan , Lei Lei , Wu Chunzhi , Jia Xiaoli , Ye Bin 
Published: June 2023

Abstract
An 81-year-old male with unresectable hepatocellular carcinoma underwent transarterial chemoembolization (TACE) combined with sorafenib. Platelet count was normal before and after TACE treatment, after which oral administration of sorafenib (400 mg po bid) was initiated. During this period, the patient experienced significant diarrhea, so the dosage was reduced to 200 mg po bid. Later, the patient showed obvious gingival bleeding with progressive exacerbation, and his blood routine examination showed a platelet count of 2 × 109 cells/L. The patient was clinically diagnosed with extreme severe thrombocytopenia. The patient was advised to stop taking sorafenib and was immediately treated with hemostasis, platelet transfusion, and suspended red blood cells. After the above treatment, the patient’s symptoms improved, and he was discharged. Up to the date of follow-up, there was no further bleeding.
Relationship between miR-7-5p expression and 125I seed implantation efficacy in pancreatic cancer and functional analysis of target genes*
Authors: Hao Tingting , Wang Chaoqi , Song Yingjie , Wu Wanyan , Li Xuetao , Fan Tao 
Published: June 2023

Abstract

Objective
The aim of this study was to investigate the relationship between miR-7-5p expression and intertissue-125I irradiation sensitivity in pancreatic cancer tissues and to analyze the function of target genes.

Methods
Thirty-seven patients with unresectable pancreatic ductal adenocarcinoma (PDAC) treated with radioactive 125I seed implantation were enrolled. RT-PCR was used to detect the expression level of miR-7-5p in cancer tissues and analyze the relationship between miR-7-5p expression and 125I radiation sensitivity. Bioinformatic software and online tools were used to predict the miR-7-5p target genes and analyze their functional annotation and pathway enrichment.

Results
Radioactive 125I seed implantation was followed up for 2 months. The objective response rate of the miR-7-5p high expression group was 65.0% (13/20), whereas the objective response rate of the miR-7-5p low expression group was 5.88% (1/17), and the difference between the two groups was statistically significant (χ
2 = 13.654, P < 0.001). A total of 187 target genes were predicted using three databases. GO functional annotation showed that target genes were mainly involved in cellular response to insulin stimulus, regulation of gene expression by genetic imprinting, cytosol, peptidyl-serine phosphorylation, bHLH transcription factor binding, cargo loading into vesicles, cellular response to epinephrine stimulus, and nucleoplasm. KEGG pathway enrichment analysis showed that target genes were mainly involved in the ErbB signaling pathway, HIF-1 signaling pathway, axon guidance, longevity regulatory pathway, endocrine resistance, glioma, choline metabolism in cancer, and EGFR tyrosine kinase inhibitor drug resistance. Molecular complex detection analysis by Cytoscape revealed that PIGH, RAF1, EGFR, NXT2, PIK3CD, PIK3R3, ERBB4, TRMT13, and C5orf22 were the key modules of miR-7-5p target gene clustering.

Conclusion
The expression of miR-7-5p in pancreatic cancer tissues positively correlated with the radiosensitivity of 125I seeds. Via targeted gene regulation, miR-7-5p acts on the network of multiple signaling pathways in PDAC and participates in its occurrence and development. Thus, miR-7-5p may become a predictive index of 125I seed implantation therapy sensitivity in PDAC patients.
Recent progress in understanding the role of genes in the pathogenesis of cutaneous squamous cell carcinoma*
Authors: He Yong , Wu Yilin , Zhang Yueyue , Lv Qun , Li Liming , Jiang Mingjun 
Published: June 2023

Abstract
Cutaneous squamous cell carcinoma (cSCC) is the second most common skin tumor in humans. Ultraviolet (UV) radiation is an important environmental risk factor for cSCC; other risk factors include human papilloma virus (HPV) infection, chronic inflammation, and chronic wounds. A large proportion of patients present with an aggressive form of cSCC at the time of diagnosis, which is often accompanied by regional lymph node involvement and distant metastases. The long-term prognosis for these highly metastatic diseases is extremely poor, with a 10-year survival rate of less than 10%. Therefore, clarifying the pathogenesis of this tumor is of great significance and may contribute to the identification of novel biomarkers and development of new therapeutic strategies. In this review, we focus on the recent progress in genes related to the development of this tumor, intending to aid future investigations into the genetic alterations related to cSCC.
Differential gene screening and functional analysis in docetaxel-resistant prostate cancer cell lines*
Authors: Wang Ming , Wang Lei , Zhang Yan , Wang Chaoqi , Li Shuang , Fan Tao 
Published: June 2023

Abstract

Objective
Docetaxel-based combination chemotherapy has traditionally been the standard treatment for metastatic castration-resistant prostate cancer (PCa). However, most patients eventually develop resistance to this treatment, which further reduces their survival. This study aimed to determine key molecular genes in docetaxel-resistant PCa cell lines using bioinformatic approaches.

Methods
The analysis of microarray data GSE33455 (including DU-145/DU-145R and PC-3/PC-3R cell lines) obtained from the Gene Expression Omnibus (GEO) database was performed using GEO2R. Differentially expressed genes (DEGs) of DU-145/DU-145R and PC-3/PC-3R cell lines were selected, and the intersection of DEGs between the two groups was obtained. DEGs were annotated with the Gene Ontology (GO) function and enriched with the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway using an online platform (https://cloud.oebiotech.cn/task/detail/array_enrichment/). The online tool Search Tool for the Retrieval of Interacting Genes (https://string-db.org/) was used to obtain the DEG network graph and matrix list, which was imported into Cytoscape 3.6.1 and analyzed using the Molecular Complex Detection plug-in to detect potential functional modules in the network.

Results
A total of 131 intersection DEGs were identified between non-treated and docetaxel-resistant PCa cell lines. GO functional annotation showed that the main genes involved were present in the plasma membrane and were involved in positive regulation of ubiquitin-protein transferase activity, positive regulation of pseudopodium assembly, centriolar subdistal appendage, and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. KEGG pathway enrichment analysis revealed that DEGs were mainly involved in IL-17 signaling pathway, cytokine-cytokine receptor interaction, rheumatoid arthritis, legionellosis, and folate biosynthesis. We identified two distinct hubs of DEGs: (1) CD274, C-X-C motif chemokine ligand (CXCL)1, DExD/H-box helicase 58, CXCL2, CXCL8, colony-stimulating factor 2, C-X-C motif chemokine receptor 4 (CXCR4), CXCL5, and CXCL6 and (2) argininosuccinate lyase, argininosuccinate synthase 1, and asparagine synthetase. Except for the CXCR4 gene that was downregulated, the other 11 genes showed upregulated expression.

Conclusion
Certain differential genes may be potential targets for predicting and treating metastatic docetaxel-resistant PCa.
Radiosensitization by microRNA30a-5p in a nude mouse model with subcutaneous lung-cancer xenograft*
Authors: Guo Yuyan , Cui Yingtao , Bao Xing , Ke Yue , Ren Hongtao , Pan Jiyuan , Song Liping , Ma Hongbing 
Published: June 2023

Abstract

Objective
We aimed to observe the radiosensitization effect of mir-30a-5p in a nude mouse model with subcutaneous lung-cancer xenograft and to explore the underlying mechanism.

Methods
A549 cell lines with either stable upregulation or downregulation of mir-30a-5p, and their negative control, were transfected with lentivirus vectors. These cell lines were used to establish a nude mouse model with subcutaneous lung-cancer xenograft. Each group was randomly divided into irradiated and non-irradiated groups. The radiosensitization effect of mir-30a-5p in vivo was studied by observing xenograft growth trends and tumor weight. The mechanisms involved in this radiosensitization were investigated by detecting expressed radiosensitization-related proteins, using immunohistochemistry and Western blotting.

Results
The expression level of mir-30a-5p in the lenti-mir-30a-5p group was higher than that in the negative control (lenti-GFP) group and lower in the lenti-inhibitor group (P < 0.05). Subcutaneous lung-cancer xenografts in the irradiation group and lenti-mir-30a-5p increased in size slowly; tumors were lighter and tumor inhibition rates were higher than those in the non-irradiation and lenti-GFP groups. In contrast, the opposite of these effects was observed in the lenti-inhibitor group. Immunohistochemistry and Western blotting indicated that ATM protein expression level was lower in the lenti-mir-30a-5p group, with or without irradiation, compared to that in the lenti-GFP group. ATM protein levels were higher in the lenti-inhibitor groups. The phosphorylation level of ATM at residue 1981 was low in the groups without irradiation and increased significantly after irradiation (P < 0.05). Moreover, the phosphorylation level was lower in the lenti-mir-30a-5p group and higher in the lenti-inhibitor group than that in the lenti-GFP group after irradiation (P < 0.05).

Conclusion
Mir-30a-5p enhanced the radiosensitivity of nude mice with subcutaneous lung-cancer xenografts by inhibiting ATM phosphorylation.
Nasal-type extranodal NK/T cell lymphoma in association with hemophagocytic syndrome: a case report and literature review*
Authors: Chen Shuang , Huang Yongchu , Cao Yuchun , Zhang Yong 
Published: June 2023

Abstract
We present a rare case of nasal-type CD56-negative NK/T-cell lymphoma. The patient developed hemophagocytic syndrome during diagnosis and treatment. The patient presented to our hospital (Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China) with “nasal congestion for 3 months and scattered erythema, nodules, and ulcers all over the body for 1 month.” We analyzed clinical manifestations, skin histopathology, immunohistochemistry, and in situ hybridization results. Histopathology of the skin revealed a moderate amount of atypical lymphocyte infiltration between the entire dermis and collagen bundles. Immunohistochemistry showed the following: CD30 (+), TIA-1 (+), CD3(2GV6) (+), CD5 part (+), CD8 part (+), CD43 (+), CD56 (-), CD4 (-), CD20 (-), PAX5 (-), PCK (-), P63 (-), P40 (-), EGFR (-), Ki-67 (the hot spot LI is approximately 80%), and in situ hybridization EBER-ROCH (+). The diagnosis made was “NK/T cell lymphoma nasal type”. This type of lymphoma is aggressive, progresses quickly, and has a poor prognosis. Early clinical manifestations are extremely atypical, especially in the absence of rash. Analysis of the skin manifestations of the disease has a positive effect on its early diagnosis, early treatment, and prognosis.
Research progress on immune checkpoint inhibitors in neoadjuvant therapy for gastric cancer
Authors: Li Wenting , Yu Shiying 
Published: June 2023

Abstract
In recent years, immune checkpoint inhibitors (ICIs) have become an important treatment strategy for advanced gastric cancer. Immunotherapy has gradually transitioned from a later-line to a first-line treatment for advanced gastric cancer. Simultaneously, more and more researchers have begun to pay attention to whether immunotherapy can be used for resectable gastric cancer. The current use of ICIs in the neoadjuvant treatment of gastric cancer is still in its exploratory stage, with a number of clinical trials currently underway. However, the available data show good application prospects. This article reviews the research progress on ICIs in the neoadjuvant therapy for gastric cancer and evokes some unresolved problems.
Analysis of the intestinal flora in patients with primary liver cancer*
Authors: Liu Chengcong , Sun Guoxin , Wang Huizhe , Shang Gaishuang , Yan Xiong , Zou Xiao 
Published: June 2023

Abstract

Objective
To investigate the differences in intestinal flora of patients with primary liver cancer and of healthy individuals and to investigate the effect of the differential flora on the development of liver cancer.

Methods
Overall, 67 patients with primary liver cancer who received systematic and complete treatment between January 2019 and December 2020 at the Sixth People’s Hospital of Qingdao and had complete clinical data were enrolled in this study, and 26 individuals who were healthy on physical examination in the same period were used as healthy controls. Macro genome and 16s ribosome Deoxyribo Nucleic Acid (rDNA) high-throughput sequencing were performed on the stool flora of the enrolled patients and controls, and the differences in the intestinal flora were analyzed using the LEfSe bioinformatics software.

Results
Compared with the control samples, all the tested patient samples showed statistically significant differences in the number of colonies of 5 bacterial phyla, 5 orders, 8 families, 11 genera, and 14 species (P < 0.05).

Conclusion
Compared with healthy people, patients with primary liver cancer have significant differences in the intestinal flora composition. The alteration of the intestinal flora may be correlated with the occurrence of primary liver cancer, and the intestinal flora may become a novel target for the prevention and treatment of primary liver cancer.