bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenolic-protein interactions: insight from in-silico analyses – a review
Authors: Shahidi Fereidoon , Dissanayaka Chandrika Sewwandi 
Published: January 2023

AbstractPhenolic compounds are ubiquitous plant secondary metabolites that possess various biological activities and are known to interact with proteins, altering their structure and properties. Therefore, interactions between these compounds and proteins has gained increasing attention due to their potential benefits to human health and for exploitation by the food industry. Phenolic compounds and proteins can form complexes via covalent linkages and/or non-covalent interactions through hydrophobic, electrostatic, van der Waals forces and hydrogen bonding. This review describes possible mechanisms of phenol-protein complex formation, their physiological action and activities that are important in the food industry, and possible outcomes in the terms of molecular docking and simulation analysis. The conformational changes of the protein upon binding with polyphenols can lead to the folding or unfolding of the protein molecules, forming insoluble or soluble complexes. The concentration of polyphenols, their molecular weight and structure, ions/cofactors and conditions of the system determine the precipitation or solubilization of the complex, affecting their nutritional and functional properties as well as their bioactivities. In this regard, molecular docking and simulation studies of phenolic-protein interactions allows comprehensive virtual screening of competitive/non-competitive and site-specific/non-specific conjugation of phenolics with different protein targets and facilitates understanding the observed effects. The docking analysis of flavonoids with enzymes and milk proteins has indicated their potential application in producing nutraceuticals and functional foods. Thus, combining molecular docking and simulation studies with experimental techniques is vital for better understanding the reactions that take place during digestion to engineer and manufacture novel food ingredients with desirable pharmacological properties and as potential food additives.
Graphical Abstract
Can triphasic hepatic multislice CT validate the LI-RADS treatment response algorithm after trans-catheter arterial chemoembolization treatment of hepatocellular carcinoma?
Authors: El-Assaly Hany , Abdelwahab Esraa , El Sebai Shady M. , Grace Marie N. 
Published: January 2023

Abstract
Background
Our aim is to evaluate LI-RADS-TR algorithm and its ability to assess the viability of TACE-treated HCC. We prospectively evaluated 100 patients with known HCC, treated with TACE and came for follow-up to assess therapy response and to plan the next step in treatment using triphasic CT study. Imaging response was evaluated according to LI-RADS-TR algorithm and compared to modified Response Evaluation Criteria in Solid Tumors (mRECIST) arterial phase hyperenhancement (APHE) criterion. Reference standard for “viable” tumors in treated observations included presence of strong tumor hyperenhancement in arterial phase and washout in the delayed phase which also shows dense accumulation of iodized oil in the target lesion.

Results
When equivocal observations were considered as LR-TR viable, LR-TR viable resulted in 92.31% sensitivity, 83.33% specificity and 88% accuracy. On the other side when equivocal observations were considered as LR-TR nonviable, it resulted in 84.62% while the specificity increased to 100% with increased accuracy (92%). The mRECIST criteria for viable tumors (presence of APHE) showed sensitivity of 84.62% and specificity of 75%. mRECIST and LR-TR sensitivities were the same when equivocal lesions were considered as nonviable and lower mRECIST than LR-TR when equivocal lesions were considered as viable, while specificities were higher in LR-TR viable being 100% when equivocal lesions were considered as nonviable, 83.33% when equivocal lesions were considered as viable and 75% in mRECIST-viable.

Conclusions
LR-TR algorithm showed good diagnostic performance compared to mRECIST, with high specificity and sensitivity when equivocal lesions were considered as nonviable, as well as improved accuracy.
Mitochondrial respiration promotes Cdc37-dependent stability of the Cdk1 homolog Cdc28
Authors: Leite Ana Cláudia , Martins Telma S. , Cesário Rute R. , Teixeira Vitor , Costa Vítor , Pereira Clara 
Published: January 2023

ABSTRACT
Cdc28, the homolog of mammalian Cdk1, is a conserved key regulatory kinase for all major cell cycle transitions in yeast. We have found that defects in mitochondrial respiration (including deletion of ATP2, an ATP synthase subunit) inhibit growth of cells carrying a degron allele of Cdc28 (cdc28td) or Cdc28 temperature-sensitive mutations (cdc28-1 and cdc28-1N) at semi-permissive temperatures. Loss of cell proliferation in the atp2Δcdc28td double mutant is associated with aggravated cell cycle arrest and mitochondrial dysfunction, including mitochondrial hyperpolarization and fragmentation. Unexpectedly, in mutants defective in mitochondrial respiration, steady-state protein levels of mutant cdc28 are strongly reduced, accounting for the aggravated growth defects. Stability of Cdc28 is promoted by the Hsp90–Cdc37 chaperone complex. Our results show that atp2Δcdc28td double-mutant cells, but not single mutants, are sensitive to chemical inhibition of the Hsp90–Cdc37 complex, and exhibit reduced levels of additional Hsp90–Cdc37 client kinases, suggesting an inhibition of this complex. In agreement, overexpression of CDC37 improved atp2Δcdc28td cell growth and Cdc28 levels. Overall, our study shows that simultaneous disturbance of mitochondrial respiration and Cdc28 activity reduces the capacity of Cdc37 to chaperone client kinases, leading to growth arrest.
Regulation of macrophage IFNγ-stimulated gene expression by the transcriptional coregulator CITED1
Authors: Subramani Aarthi , Hite Maria E. L. , Garcia Sarah , Maxwell Jack , Kondee Hursha , Millican Grace E. , McClelland Erin E. , Seipelt-Thiemann Rebecca L. , Nelson David E. 
Published: January 2023

ABSTRACT
Macrophages serve as a first line of defense against microbial pathogens. Exposure to interferon-γ (IFNγ) increases interferon-stimulated gene (ISG) expression in these cells, resulting in enhanced antimicrobial and proinflammatory activity. Although this response must be sufficiently vigorous to ensure the successful clearance of pathogens, it must also be carefully regulated to prevent tissue damage. This is controlled in part by CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 2 (CITED2), a transcriptional coregulator that limits ISG expression by inhibiting STAT1 and IRF1. Here, we show that the closely related Cited1 is an ISG, which is expressed in a STAT1-dependent manner, and that IFNγ stimulates the nuclear accumulation of CITED1 protein. In contrast to CITED2, ectopic CITED1 enhanced the expression of a subset of ISGs, including Ccl2, Ifit3b, Isg15 and Oas2. This effect was reversed in a Cited1-null cell line produced by CRISPR-based genomic editing. Collectively, these data show that CITED1 maintains proinflammatory gene expression during periods of prolonged IFNγ exposure and suggest that there is an antagonistic relationship between CITED proteins in the regulation of macrophage inflammatory function.
This article has an associated First Person interview with the first author of the paper.
Education of students in higher educational medical universities of Ukraine taking into account competence-oriented educational technologies
Authors: Batyuk Liliya , Zhernovnykova Oksana 
Published: January 2023

A higher educational institution is called upon to contribute to a constant increase in the degree of development and readiness of students for self-educational activities on a proactive basis. The features of training and formation of competences among medical students in the general theoretical aspect are considered as a fundamental condition for the successful implementation of future professional activities, which provides the ability to quickly and correctly use knowledge, experience, and personal qualities, ensures their control and restructuring of activities when obstacles appear.
The development of the proposed theoretical and practical material in medical universities is of a general nature, including and substantiating the goal, objectives and structure of the formation of the personality of a future professional. The application of the competence-based approach and the practical orientation of training in higher educational medical institutions of Ukraine are designed to form student’s not only preliminary, but also situational readiness.
In the context of considering competence-oriented educational technologies in higher educational universities of Ukraine, including pedagogical and educational technologies, is the readiness of future specialists for productive independent and responsible action in professional activities and everyday life. The teachers of medical universities are obliged to ensure the achievement of this result using a complex integral image of the final result of education at the university in the direction of training (specialty), which is based on the concept of “competence”.
Awareness of the costs of selected medical procedures and the use of healthcare among young adults in Poland
Authors: Rogalska Anna , Sobczyk Karolina , Holecki Tomasz 
Published: January 2023

Aim: The study aimed to test students' awareness of the costs of selected medical procedures. Another aim was to identify the frequency of used medical benefits both as part of public and private medical care.
Materials and Methods: The research group included 246 people, both women, and men, aged 19-29, studying in Poland. An anonymous research questionnaire was used to conduct the study, which was sent to 21 universities in Poland (medical universities and physical education academies). The questionnaire asked participants to estimate the costs of treatments and investigations. The respondents were also asked which services of medical specialists they used in the last year.
Results: Outpatient specialist care services under the public health care system were used by the majority of respondents (N = 200; 81.3%). Most often it was 1-2 times in the last year (54.5% of all respondents used the public health service). The respondents most often used gynecological (33%), ophthalmological (21%), and dermatological (15.5%). In turn, private medical care was used by 192 respondents (78%) out of all the respondents. Respondents revalued all assessed medical procedures by 27% for the diagnostic colonoscopy to 790% for a 20-minute massage at a rehabilitation clinic.
Conclusion: Cost awareness among polish students is poor. Lack of awareness of the costs of medical procedures (the amount that the public payer pays for specific procedures medical) that may have an impact on the use of healthcare services as well as health care expenses.
Acute Disseminated Encephalomyelitis Associated with Cytomegalovirus Infection in an Immunocompetent Subject
Authors: Shen Hsiu-Chu , Lin Chien-Jen , Lin Huey-Juan 
Published: January 2023

Purpose: Fulminant acute disseminated encephalomyelitis (ADEM) associated with cytomegalovirus (CMV) infection in immunocompetent hosts is rare, and the MRI findings might be atypical. Case report: A 17-year-old immunocompetent man presented with acute flaccid paraplegia accompanied by bladder and bowel incontinence. The serum virology tests revealed the presence of CMV IgM and IgG, indicating reactivation of latent infection. MRI studies of the brain and the whole spinal cord showed diffuse gray matter lesions of hyperintense signals in T2. After aggressive rescue therapy combining intravenous immunoglobulin, pulse methylprednisolone and plasma exchange, the patient made a complete neurological recovery 6 months later. Conclusion: This report demonstrates the association of CMV infection with ADEM in an immunocompetent subject. Although the treatment regimen and timing for patients with ADEM need further investigation, aggressive rescue therapy by adopting multiple immunomodulatory treatments may be warranted.
Trends in Low-Density Lipoproteins Cholesterol (LDL-C) in US Aged 12 to 80 Years: Data from the 2007-2018 National Health and Nutrition Examination Survey (NHANES)
Authors: Wang Inga , Hou Stephen , Rahman Mohammad H , Li Xiaoyan , Ritchie David , Spector Antoinette L 
Published: January 2023

Background: Elevated levels of low-density lipoprotein cholesterol (LDL-C) are associated with an increased risk of coronary heart disease.
Objectives: We described LDL-C distributions across the age span and compared the trends in three distinct cross-sectional surveys during 2007-2010, 2011-2014, and 2015-2018.
Methods: This is an observational study. Blood lipid measurements, taken from 40,977 noninstitutionalized participants, aged 12 to 80, were obtained from the National Health and Nutrition Examination Survey (NHANES) study.
Results: The LDL-C values changed by age and differed by sex. Mean LDL-C levels increased with age through middle age and then decreased with age. In males, mean LDL-C declined from 104.5 (95% CI, 103.6-105.3) in 2007-2010, to 100.5 (95% CI, 99.7-101.4) in 2011-2014, and 100.8 (95% CI, 99.9-101.7) in 2015-2018 (p<0.001 for linear trend). In females, mean LDL-C declined from 110.3 (95% CI, 109.5-111.1) in 2007-2010, to 108.3 (95% CI, 107.6-109.1) in 2011-2014, and 106.8 (95% CI, 105.9-107.7) in 2015-2018 (p<0.001 for linear trend).
Conclusions: Between 2007 and 2018, favorable trends in LDL-C levels were observed among noninstitutionalized residents in the US.
Peripheral Giant Cell Granuloma in Edentulous Region
Authors: Zapata-Domínguez C. , Monzón-Lloret M. , Schiavone-Mussano R. , Jané-Salas E. , Devesa-Estrugo A. , López-López J. 
Published: January 2023

The peripheral giant cell granuloma (PGCG) is a benign exophytic lesion of the oral cavity. Its etiology is unknown but is associated with a local irritative or aggressive factor. The highest incidence is observed between 40-60 years, although lesions have been reported at all ages and are most commonly associated with the female sex. The treatment consists of complete excision and subsequent pathological examination of the lesion.
The aim of this paper is to present the diagnosis and treatment of a case of peripheral giant cell granuloma (PGCG). The success of the case as well as its clinical and radiographic follow-up is discussed in relation to the published literature.
Stem Cells from Human Exfoliated Deciduous Teeth in Delayed Tooth Replantation: Histologic and Histomorfometric Analyses in Rats
Authors: Ventura Marsha Lisa Schlittler , Bisol Ângela , Rasmussen Lucas Trevizani , De Labio Roger Willian , Segato Rosemeire , Viani Gustavo , Dos Santos Ricardo Ribeiro , Okamoto Tetuo , Okamoto Roberta , Sonoda Celso , Payão Spencer Luiz Marques 
Published: January 2023

Aim: The complication in delayed replantation is root resorption that leads to loss of reimplanted teeth. This study was designed to evaluate the influence of stem cells from human exfoliated deciduous teeth (SHEDs) on the healing process of periodontal tissues after delayed tooth replantation in rats.
Material and Methods: Forty-five rats had their right upper incisor extracted and maintained in dry storage for 60 minutes. Then dental papilla was removed; root canal was prepared and filled with calcium hydroxide paste and root surface treatment was processed. Rats were randomly assigned into three groups: Control group: the incisor was replanted without any substance in the dental socket. SHEDs group: the tooth was replanted after infusion of SHED, re-suspended in platelet-rich-fibrin in the dental socket; platelet-rich-fibrin group: the incisor was replanted with platelet-rich-fibrin in the dental socket. Animals were euthanized on days 15, 30 and 60 after replantation; histological and histomorphometric analyses were performed.
Results: Root resorption was present in all experimental groups. No statistically significant differences were observed in the final period of study. The control group showed more replacement resorption but SHEDs group, showed dental tissues were more preserved. Inflammatory resorption nearly did not affect any group, as expected in this protocol (p<0.005).
Conclusions: The findings of this study suggest that in the SHEDs group, teeth were more preserved with ankyloses occurrence, witch holds teeth in the socket for a longer period of time, when compared to replacement resorption or inflammatory resorption in the healing process of delayed replantation.
Theta Frequency is Associated to Morpho-Strcutural and Perfusional Modifications in Subjects with Mild Cognitive Impairment
Authors: Moretti D.V. , Paternicò D. , Prestia A. , Binetti G. , Zanetti O. , Frisoni G.B. 
Published: January 2023

Background: In an attempt to find non-invasive biomarkers, researchers have investigated the feasibility of neuroimaging tools, such as MR, SPECT as well as neurophysiological measurements using EEG. The increase of theta frequency has been associated with mild cognitive impairment (MCI) and related to both grey matter (GM) changes of thalamus and basal ganglia and SPECT modifications. Objective: To study the association of prognostic theta frequency with specific GM and perfusional changes of thalamus and basal ganglia to detect biomarkers early predictive of mild cognitive impairment. Methods: 74 adult subjects with mild cognitive impairment underwent EEG recording and high resolution 3D magnetic resonance imaging (MRI). Moreover, 27 adult subjects with mild cognitive impairment underwent also perfusion single-photon emission computed tomography (SPECT) evaluation. The theta/gamma ratio was computed for each subject. Three groups were obtained according to increasing tertiles values of theta/gamma ratio. Grey matter density differences between groups were investigated using a Voxel Based Morphometry technique. Results: Subjects with higher theta/gamma ratio and increase of theta frequency showed minor atrophy in putamina nuclei bilaterally and a lower hippocampal perfusion in subjects with mild cognitive impairment. Conclusion: The integrated analysis of EEG and morpho-functional markers could be useful in the comprehension of anatomo-physiological underpinning of the MCI entity.
Improving the upper atmospheric temperature accuracy of the ground-based instrument by eliminating noise ways
Authors: Xue Yangyang , Li Cunxia , Hui Ningju , Liu Lina , Tong Junyi , Zhang Rong , Tang Yuanhe 
Published: January 2023

In view of the special properties of the upper atmosphere at the altitude of
80–120 km, a ground-based passive remote sensing instrument
ground-based airglow volume emission rate and temperature imaging
interferometer (GBAVTII) is built to detect the atmospheric
temperature used

O

2

(

0
-
1

)

spectral line of night airglow at the
altitude of 94 km. In the process of photographing the upper
atmosphere airglow with the GBAVTII, the stray light (white noise)
such as moonlight, city lights, and starlight will be affected. In
this paper, the theoretical expression of denoising is derived based
on the rotational line temperature measurement of diatomic

O

2

(

0
-
1

)

airglow. Through a slight adjustment
of different parameters in the forward equation of the GBAVTII and
noise reduction in laboratory flat-field fine calibration, and other
denoising methods in the GBAVTII image processing process, the maximum
accuracy of the GBAVTII detection of the upper atmospheric temperature
is enhanced to 2.4 K. Also, the minimum error of the GBAVTII
detecting data with the satellite instrument sounding of atmosphere
using broadband emission radiometry is 0.4 K. Thus, the
absolute accuracy of the GBAVTII in detecting the upper atmospheric
temperature can be improved to

±

(

0.4
−

2.4

)

K

through the theory and method studied
in this paper.
Single LED visible light positioning system based on image sensor and calculated azimuth angle
Authors: Wang Tongyao , Lin Zuojun , Cheng Han , Xiao Chunxian 
Published: January 2023

A single-lamp visible light positioning (VLP) system based on an optical camera is proposed and demonstrated. The system uses a circular light-emitting diode (LED) to transmit ID data, and a common smartphone camera for positioning. First, we propose a scheme to calculate the azimuth angle using a marked lamp bead, avoiding the use of a magnetometer, which can improve the accuracy of the azimuth angle. Second, we propose a new positioning method using a chord in the image plane, which passes through the midpoints of two chords parallel to the LED plane. The experimental results show that when the experimental area is

1.8

m

×
3.6

m

, the three-dimensional (3D) average positioning errors are 8.0, 7.5, and 7.2 cm for the heights of 1.75, 1.45, and 1.2 m.
Sandwich structure magnetometer with a high sensitivity and signal-to-noise ratio based on an ultrahigh-Q CaF2 resonator
Authors: Bai Jiamin , Rong Jiamin , Xing Enbo , Ji Ruipu , Yue Jun , Li Li , Liu Wenyao , Zhou Yanru , Tang Jun , Liu Jun 
Published: January 2023

Whispering gallery mode (WGM)
resonators with an ultrahigh quality (

Q

) factor provide an extremely high
resolution for high-precision sensing. However, it is difficult to use
them directly in magnetic sensors because of the transparency to the
magnetic field. In this paper, a sandwich structure consisting of a
neodymium iron boron magnet and calcium fluoride resonator with a

Q

factor of

10

9

is proposed. The experimental results
show that, compared with the conventional magnetometer, the
signal-to-noise ratio of the optical WGM magnetometer reaches
62 dB, with the direct current sensitivity of
42.59 MHz/mT and the AC sensitivity of

794

p
T

H
z

−
1

/

2

at 42 kHz.
Efficient second-harmonic generation through cascaded optically poled fibers
Authors: Gemechu Wasyhun Asefa , Minoni Umberto , Modotto Daniele , Tonello Alessandro , Couderc Vincent 
Published: January 2023

We report the experimental demonstration of efficient second-harmonic generation by splicing optically poled fiber segments. A device made from five segments each 20 cm-long exhibits, at a fundamental average power of 4.2 mW, a maximum increase of 5.5 in the conversion efficiency with respect to a single 20 cm poled fiber, corresponding to a conversion efficiency of 5.4%. For any considered fundamental power, the conversion efficiency at the output of a five-segment device is larger than the efficiency of a single poled fiber having the same length.