bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stakeholder perspectives on cooperation in the clinical and nonclinical health technology assessment domains
Authors: Zimmermann Gina , Michelmore Sandra , Hiligsmann Mickaël 
Published: May 2023

Abstract

Objectives
The aim of this study was to deliver insights from multiple stakeholders into actual and future collaboration for health technology assessment (HTA) in general and in oncology in particular.

Methods
Eighteen semi-structured interviews were conducted with experts from European HTA bodies (HTAbs), former board members of the European Network for Health Technology Assessment (EUnetHTA), and representatives from the pharmaceutical industry, a regulatory agency, academia, and patient organizations. The stakeholders were asked about their support of the EUnetHTA’s intent, about the general strengths and challenges of the EUnetHTA and its Joint Action 3 (JA 3), the strengths and challenges of the clinically oriented HTA collaboration in oncology during JA 3 across the technology life cycle, about future challenges to HTA in oncology with consequences for collaboration, and about collaboration in the economic domains of HTA. The transcribed interviews were analyzed qualitatively.

Results
The participants perceived the intention and work quality of the EUnetHTA as positive. The experts described methodological, procedural, and capacity challenges in early dialogues (EDs) and rapid relative effectiveness assessments (REAs) meant to analyze clinical effectiveness in oncology. The majority attached increasing importance to collaboration in the future to cope with the uncertainty of HTA. Several stakeholders also proposed the incorporation of joint postlaunch evidence generation (PLEG) activities. Some gave sporadic suggestions for voluntary nonclinical collaboration as well.

Conclusion
Stakeholders’ continued readiness to discuss the remaining challenges to and sufficient resources for implementing HTA regulation, as well as further cooperative expansion along the technology life cycle, are necessary for improved HTA collaboration in Europe.
Genomic analysis of an outbreak of toxin gene bearing Corynebacterium diphtheriae in Northern Queensland, Australia reveals high level of genetic similarity
Authors: Graham Rikki M. A. , Rathnayake Irani U. , Sandhu Sumeet , Bhandari Murari , Taunton Caroline , Fisher Valmay , Hempenstall Allison , Marquardt Tonia , Jennison Amy V. 
Published: May 2023

Abstract
Toxigenic diphtheria is rare in Australia with generally fewer than 10 cases reported annually; however, since 2020, there has been an increase in toxin gene-bearing isolates of Corynebacterium diphtheriae cases in North Queensland, with an approximately 300% escalation in cases in 2022. Genomic analysis on both toxin gene-bearing and non-toxin gene-bearing C. diphtheriae isolated from this region between 2017 and 2022 demonstrated that the surge in cases was largely due to one sequence type (ST), ST381, all of which carried the toxin gene. ST381 isolates collected between 2020 and 2022 were highly genetically related to each other, and less closely related to ST381 isolates collected prior to 2020. The most common ST in non-toxin gene-bearing isolates from North Queensland was ST39, an ST that has also been increasing in numbers since 2018. Phylogenetic analysis demonstrated that ST381 isolates were not closely related to any of the non-toxin gene-bearing isolates collected from this region, suggesting that the increase in toxigenic C. diphtheriae is likely due to the expansion of a toxin gene-bearing clone that has moved into the region rather than an already endemic non-toxigenic strain acquiring the toxin gene.
Exonic genetic variants associated with unexpected fertilization failure and zygotic arrest after ICSI: a systematic review
Authors: Torra-Massana Marc , Rodríguez Amelia , Vassena Rita 
Published: May 2023

SummaryFertilization failure (FF) and zygotic arrest after ICSI have a huge effect on both patients and clinicians, but both problems are usually unexpected and cannot be properly diagnosed. Fortunately, in recent years, gene sequencing has allowed the identification of multiple genetic variants underlying failed ICSI outcomes, but the use of this approach is still far from routine in the fertility clinic. In this systematic review, the genetic variants associated with FF, abnormal fertilization and/or zygotic arrest after ICSI are compiled and analyzed. Forty-seven studies were included. Data from 141 patients carrying 121 genetic variants affecting 16 genes were recorded and analyzed. In total, 27 variants inPLCZ1(in 50 men) and 26 variants inWEE2(in 24 women) are two of the factors related to oocyte activation failure that could explain a high percentage of male-related and female-related FF. Additional variants identified were reported inWBP2NL,ACTL9, ACTLA7,andDNAH17(in men), andTUBB8,PATL2,TLE6,PADI6,TRIP13,BGT4, NLRP5, NLRP7,CDC20andZAR1(in women). Most of these variants are pathogenic or potentially pathogenic (89/121, 72.9%), as demonstrated by experimental and/orin silicoapproaches. Most individuals carried bi-allelic variants (89/141, 63.1%), but pathogenic variants in heterozygosity have been identified forPLCZ1andTUBB8. Clinical treatment options for affected individuals, such as chemical-assisted oocyte activation (AOA) orPLCZ1cRNA injection in the oocyte, are still experimental. In conclusion, a genetic study of known pathogenic variants may help in diagnosing recurrent FF and zygotic arrest and guide patient counselling and future research perspectives.
Start codon-targeted marker evaluation of genetic relationship and population structure in southern Nigerian fluted pumpkin (Telfairia occidentalis Hook F.) collection
Authors: Aworunse Oluwadurotimi S. , Popoola Jacob O. , Paliwal Rajneesh , Obembe Olawole O. 
Published: May 2023

AbstractFluted pumpkin (Telfairia occidentalis Hook F.) is an underutilized indigenous leafy vegetable with enormous prospects for food security in sub-Saharan Africa. However, relatively little is known about genetic relationships and population structure in the species. In this study, 32 landraces of fluted pumpkin collected across three southern geographical regions in Nigeria were assessed for genetic diversity and population structure using 8 start codon-targeted (SCoT) makers. The polymorphic information content of the SCoT markers ranged from 0.48 in SCoT36 to 0.94 in SCoT28, with an average of 0.77. Hierarchical cluster dendrogram based on Ward's method and principal component analysis grouped the landraces into four clusters without affiliation to provenance. Overall, the mean values of the population genetic diversity parameters – Nei's gene diversity (H) and Shannon's information index (I) showed values of 0.28 ± 0.01 and 0.43 ± 0.02, respectively, implying a narrow genetic base for the landraces. The result was further corroborated by a very close Nei's genetic distance and identity among populations of the landraces. Furthermore, the south-west population exhibited the higher genetic diversity (H = 0.31 ± 0.02 and I = 0.45 ± 0.03). Population structure analysis inferred three subpopulations for the accessions with varying degrees of allelic admixture. An analysis of molecular variance revealed that almost all the genetic variation occurred within (99%) than between (1%) populations. The findings shed light on the genetic diversity of southern Nigerian fluted pumpkin and have significant implications for the characterisation, conservation, exploitation and improvement of the species.
VARIETIES OF CLASS-THEORETIC POTENTIALISM
Authors: BARTON NEIL , WILLIAMS KAMERYN J. 
Published: May 2023

AbstractWe explain and explore class-theoretic potentialism—the view that one can always individuate more classes over a set-theoretic universe. We examine some motivations for class-theoretic potentialism, before proving some results concerning the relevant potentialist systems (in particular exhibiting failures of the
$\mathsf {.2}$
and
$\mathsf {.3}$
axioms). We then discuss the significance of these results for the different kinds of class-theoretic potentialists.
On the Scaling and Growth Limit of Fluvial Dunes
Authors: Lee Jiyong , Singh Arvind , Guala Michele 
Published: May 2023

AbstractBedforms emerge in a variety of shapes and sizes when a granular erodible surface is subject to a strong enough shear flow, as observed in topographic data from submarine canyons, rivers, deserts, and planetary bodies. The two salient features of bedforms are the ability to collectively transport particles, and to generate form drag thus increasing flow resistance. These two mechanisms are in competition and contribute to force bedforms of increasing size migrating more slowly. In a dedicated large‐scale open channel flow facility, hierarchies of fluvial bedforms were generated and measured in equilibrium conditions. The corresponding scale‐dependent migration velocity and mass flux contributions were quantified in the wave number and frequency domains. Experimental results are paired with a validated set of theoretical models to demonstrate that ripples or dunes reach an equilibrium state when drag partitioning ensures high enough frictional drag to sustain the bedload transport of sediment, and low enough form drag to enable the migration of the largest bedform size. This mechanism is inferred to constrain the growth of bedforms when sediment supply is not the limiting factor.
Changes in Non‐Dipolar Field Structure Over the Plio‐Pleistocene: New Paleointensity Results From Hawai'i Compared to Global Data Sets
Authors: Cych Brendan , Tauxe Lisa , Cromwell Geoffrey , Sinton John , Koppers Anthony A. P. 
Published: May 2023

AbstractA foundational assumption in paleomagnetism is that the Earth's magnetic field behaves as a geocentric axial dipole (GAD) when averaged over sufficient timescales. Compilations of directional data averaged over the past 5 Ma yield a distribution largely compatible with GAD, but the distribution of paleointensity data over this timescale is incompatible. Reasons for the failure of GAD include: (a) Arbitrary “selection criteria” to eliminate “unreliable” data vary among studies, so the paleointensity database may include biased results. (b) The age distribution of existing paleointensity data varies with latitude, so different latitudinal averages represent different time periods. (c) The time‐averaged field could be truly non‐dipolar. Here, we present a consistent methodology for analyzing paleointensity results and comparing time‐averaged paleointensities from different studies. We apply it to data from Plio/Pleistocene Hawai'ian igneous rocks, sampled from fine‐grained, quickly cooled material (lava flow tops, dike margins and scoria cones) and subjected to the IZZI‐Thellier technique; the data were analyzed using the Bias Corrected Estimation of Paleointensity method of Cych et al. (2021, https://doi.org/10.1029/2021GC009755), which produces accurate paleointensity estimates without arbitrarily excluding specimens from the analysis. We constructed a paleointensity curve for Hawai'i over the Plio/Pleistocene using the method of Livermore et al. (2018, https://doi.org/10.1093/gji/ggy383), which accounts for the age distribution of data. We demonstrate that even with the large uncertainties associated with obtaining a mean field from temporally sparse data, our average paleointensities obtained from Hawai'i and Antarctica (reanalyzed from Asefaw et al., 2021, https://doi.org/10.1029/2020JB020834) are not GAD‐like from 0 to 1.5 Ma but may be prior to that.
External Dynamic Mechanisms Controlling the Periodic Offshore Blooms in Beibu Gulf
Authors: Lao Qibin , Liu Sihai , Ling Zheng , Jin Guangzhe , Chen Fajin , Chen Chunqing , Zhu Qingmei 
Published: May 2023

AbstractSince offshore waters are less affected by human activities and nutrient‐rich water masses, existing theories on periodic offshore blooms (POB) consider that the POB is proportional to the intensity of ocean fronts (nutrient supply from enhancing vertical mixing), ignoring external nutrient supply and external forcing (climatic oscillations). This study proposes an external dynamic mechanism of the POB on the basis of field observations and long‐term satellite remote sensing data (1981–2022) in Beibu Gulf, which is influenced by remarkable external forcing. A strong thermal front with an inverted‐V structure occurred in the central gulf every winter due to the strong wind stress. The intensity of the front on the east side is weaker due to the intrusion of the west‐Guangdong coastal current (WGCC). However, nutrient supply variation resulted in the POB's different intensity in different areas of the inverted‐V front. Chlorophyll a concentrations in the eastern (nutrients supplied by WGCC) and northern (nutrients supplied by vertical mixing) were obviously higher than that in the western front (limited nutrient supply) in winter. On an interannual scale, the intensity of POB in La Niña years is remarkably stronger than in El Niño years due to the stronger WGCC supplying more nutrients in La Niña. This study suggests that the intensity and range of POB are not proportional to the frontal intensity in the gulf, but are directly driven by the internal forcing (fronts and nutrient supply from WGCC), which is controlled by the external forcing.
Seg2Link: an efficient and versatile solution for semi-automatic cell segmentation in 3D image stacks
Authors: Wen Chentao , Matsumoto Mami , Sawada Masato , Sawamoto Kazunobu , Kimura Koutarou D. 
Published: May 2023

AbstractRecent advances in microscopy techniques, especially in electron microscopy, are transforming biomedical studies by acquiring large quantities of high-precision 3D cell image stacks. To examine cell morphology and connectivity in organs such as the brain, scientists need to conduct cell segmentation, which extracts individual cell regions of different shapes and sizes from a 3D image. This is challenging due to the indistinct images often encountered in real biomedical research: in many cases, automatic segmentation methods inevitably contain numerous mistakes in the segmentation results, even when using advanced deep learning methods. To analyze 3D cell images effectively, a semi-automated software solution is needed that combines powerful deep learning techniques with the ability to perform post-processing, generate accurate segmentations, and incorporate manual corrections. To address this gap, we developed Seg2Link, which takes deep learning predictions as inputs and use watershed 2D + cross-slice linking to generate more accurate automatic segmentations than previous methods. Additionally, it provides various manual correction tools essential for correcting mistakes in 3D segmentation results. Moreover, our software has been optimized for efficiently processing large 3D images in diverse organisms. Thus, Seg2Link offers an practical solution for scientists to study cell morphology and connectivity in 3D image stacks.
Determination of optimum intensity and duration of exercise based on the immune system response using a machine-learning model
Authors: Asadi Shirin , Tartibian Bakhtyar , Moni Mohammad Ali 
Published: May 2023

AbstractOne of the important concerns in the field of exercise immunology is determining the appropriate intensity and duration of exercise to prevent suppression of the immune system. Adopting a reliable approach to predict the number of white blood cells (WBCs) during exercise can help to identify the appropriate intensity and duration. Therefore, this study was designed to predict leukocyte levels during exercise with the application of a machine-learning model. We used a random forest (RF) model to predict the number of lymphocytes (LYMPH), neutrophils (NEU), monocytes (MON), eosinophils, basophils, and WBC. Intensity and duration of exercise, WBCs values before exercise training, body mass index (BMI), and maximal aerobic capacity (VO2 max) were used as inputs and WBCs values after exercise training were assessed as outputs of the RF model. In this study, the data was collected from 200 eligible people and K-fold cross-validation was used to train and test the model. Finally, model efficiency was assessed using standard statistics (root mean square error (RMSE), mean absolute error (MAE), relative absolute error (RAE), root relative square error (RRSE), coefficient of determination (R2), and Nash–Sutcliffe efficiency coefficient (NSE)). Our findings revealed that the RF model performed well for predicting the number of WBC with RMSE = 0.94, MAE = 0.76, RAE = 48.54, RRSE = 48.17, NSE = 0.76, and R2 = 0.77. Furthermore, the results showed that intensity and duration of exercise are more effective parameters than BMI and VO2 max to predict the number of LYMPH, NEU, MON, and WBC during exercise. Totally, this study developed a novel approach based on the RF model using the relevant and accessible variables to predict WBCs during exercise. The proposed method can be applied as a promising and cost-effective tool for determining the correct intensity and duration of exercise in healthy people according to the body’s immune system response.
The retinal neurovascular coupling is impaired in men with vasculogenic erectile dysfunction
Authors: Borrelli Enrico , Quarta Alberto , Pozzi Edoardo , Fallara Giuseppe , Pennisi Flavia , Casaluci Marco , Lamanna Francesca , Querques Lea , Sacconi Riccardo , Bandello Francesco , Montorsi Francesco , Salonia Andrea , Querques Giuseppe 
Published: May 2023

AbstractThe aim of this study was to study the retinal vessels in patients affected by vasculogenic erectile dysfunction (ED), using dynamic vessel analyzer (DVA). Patients with vasculogenic ED and control subjects were prospectively enrolled to undergo a complete urological and ophthalmologic evaluation, including DVA and structural optical coherence tomography (OCT). The main outcome measures were: (1) arterial dilation; (2) arterial constriction; (3) reaction amplitude (the difference between arterial dilation and constriction); and, (4) venous dilation. Thirty-five patients with ED and 30 male controls were included in the analysis. Mean ± SD age was 52.0 ± 10.8 years in the ED group and 48.1 ± 16.3 years in the control group (p = 0.317). In the dynamic analysis, the arterial dilation was lower in the ED group (1.88 ± 1.50%), as compared with the control group (3.70 ± 1.56%, p < 0.0001). Neither arterial constriction nor venous dilation differed between groups. The reaction amplitude was decreased in ED patients (2.40 ± 2.02%, p = 0.023), compared to controls (4.25 ± 2.20%). In the Pearson correlation analysis, the ED severity, was directly correlated with both reaction amplitude (R = .701, p = 0.004) and arterial dilation (R = .529, p = 0.042). In conclusion, subjects with vasculogenic ED are featured by a significant dysfunction of the retinal neurovascular coupling, which is inversely correlated with ED severity.
Social deprivation in maternal mouthbrooders Tropheus sp. “Caramba” (Teleostei: Cichlidae) decreases the success rate of reproduction and survival rate of fish fry
Authors: Novák Jindřich , Frynta Daniel , Nováková Daniela , Patoka Jiří 
Published: May 2023

AbstractEarly offspring separation from mothers causes social deprivation. Mouthbrooding, when eggs and fry are incubated in the buccal cavity of the parent, is one of the reproductive strategies in fish. The mother is the incubating parent in African lake cichlids from the genus Tropheus. Many of these are produced in captivity and some producers use artificial incubators in which eggs are incubated separately from the mother. We hypothesized that this practice may dramatically modify the reproduction rate of fish individuals produced by the method of artificial incubation. The long-term experiment focused on Tropheus sp. “Caramba” had been carried out for 10 years when maternally incubated and separated individuals were compared. We found a negative effect of artificial egg and offspring incubation out of the mother’s buccal cavity. The deprived females laid the same number of eggs as maternally incubated females, but most eggs were lost during the incubation. Moreover, the reproduction frequency was significantly lower in deprived females in comparison with those maternally incubated. This study should be perceived as preliminary. For this reason and with respect to welfare principles, we strongly recommend similarly designed experiments focused on other potentially sensitive fish mouthbrooders. Once the syndrome would be confirmed, we recommend avoiding artificial incubation of mouthbrooding fish in general.
Improving stress management, anxiety, and mental well-being in medical students through an online Mindfulness-Based Intervention: a randomized study
Authors: Fazia Teresa , Bubbico Francesco , Nova Andrea , Buizza Chiara , Cela Herald , Iozzi Davide , Calgan Beril , Maggi Federica , Floris Valentina , Sutti Irene , Bruno Salvatore , Ghilardi Alberto , Bernardinelli Luisa 
Published: May 2023

AbstractPressures and responsibilities of medical school put a strain on medical student's personal wellbeing, leading among all to high rates of anxiety, emotional discomfort and stress. In this work we evaluated the effectiveness of a comprehensive Mindfulness-Based Intervention (MBI) in reducing this load. The intervention comprised 10 twice-a-week Integral Meditation classes, dietary advice, and brief yoga sessions. We performed a randomized trial on two cohort of medical students from Italian universities: 239 in cohort 1 (106 treated and 133 controls), and 123 in cohort 2 (68 treated and 55 control) for a total sample of 362 students. Nine questionnaires for evaluating the effectiveness of our intervention on stress (PSS), state anxiety (STAIX-1), well-being (WEMWBS), mind-wandering (MW-S), overall distress (PANAS), emotion regulation (DERS), resilience (RS-14), and attentional control (ACS-C and ACS-D) were collected both pre and post intervention. Linear mixed effect models were run on the whole sample showing that, after multiple testing correction, our intervention was effective in reducing perceived stress (β = − 2.57 [− 4.02; − 1.12], p = 0.004), improving mental well-being (β = 2.82 [1.02; 4.63], p = 0.008) and emotional regulation (β = − 8.24 [− 12.98; − 3.51], p = 0.004), resilience (β = 3.79 [1.32; 6.26], p = 0.008), reducing the tendency to wander with the mind (β = − 0.70 [− 0.99; − 0.39], p = 0.0001), ameliorating the ability to maintain attention (AC-S (β = − 0.23 [− 0.44; − 0.02], p = 0.04) and AC-D (β = − 0.19 [− 0.36; − 0.01], p = 0.04)), and the overall distress (β = 1.84 [0.45; 3.23], p = 0.02).
Quantifying the human cost of global warming
Authors: Lenton Timothy M. , Xu Chi , Abrams Jesse F. , Ghadiali Ashish , Loriani Sina , Sakschewski Boris , Zimm Caroline , Ebi Kristie L. , Dunn Robert R. , Svenning Jens-Christian , Scheffer Marten 
Published: May 2023

AbstractThe costs of climate change are often estimated in monetary terms, but this raises ethical issues. Here we express them in terms of numbers of people left outside the ‘human climate niche’—defined as the historically highly conserved distribution of relative human population density with respect to mean annual temperature. We show that climate change has already put ~9% of people (>600 million) outside this niche. By end-of-century (2080–2100), current policies leading to around 2.7 °C global warming could leave one-third (22–39%) of people outside the niche. Reducing global warming from 2.7 to 1.5 °C results in a ~5-fold decrease in the population exposed to unprecedented heat (mean annual temperature ≥29 °C). The lifetime emissions of ~3.5 global average citizens today (or ~1.2 average US citizens) expose one future person to unprecedented heat by end-of-century. That person comes from a place where emissions today are around half of the global average. These results highlight the need for more decisive policy action to limit the human costs and inequities of climate change.