bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excellent condensate and salt‐resistant foam by anionic‐zwitterionic‐cationic Gemini surfactants compounds for gas well deliquification
Authors: Dong Sanbao , Fan Jiabao , Lv Hongmiao , Qiang Taotao , Ji Yue , Han Weiwei 
Published: September 2023

AbstractThe blockage caused by liquid loading poses a major problem to the aged gas wells. Various categories of foaming surfactants including zwitterionic, anionic and cationic surfactants are often used for foam‐based gas well deliquification. In this study, a foaming formula containing anionic (sodium lauroyl sarcosinate, LSS), cationic Gemini (C16AGB and C12AGB) and zwitterionic (C16APB and C12APB) surfactants was developed. This foaming formula was first optimized through the foaming ability and foam stability tests, and then evaluated through liquid unloading performance tests under various salinity, condensate, methanol and temperature conditions. Synergy was observed in the foaming ability and foam stability measurements when LSS, C16AGB, C12AGB, C16APB and C12APB were combined at a molar ratio of 5:4:1:4:1. The foaming formula exhibited excellent liquid unloading performance at high salinity (up to 26 × 104 mg/L), high condensate fraction (up to 25%) and high methanol fraction (up to 50%) conditions. The presence of 10% methanol significantly enhanced foaming efficiency (referred to as the ratio of the foam mass to the initial liquid mass) and liquid unloading efficiency of the foaming formula at 20–90°C. Furthermore, the surface tension measurements and the foam morphological investigation were carried out to reveal the observed synergy.
Screening of novel bovine‐elastin‐derived peptides with elastase inhibition and photoprotective potential: a combined in silico and in vitro study
Authors: Wu Jing , Li Qinglan , Cui Chun , Xu Jucai 
Published: September 2023

AbstractBACKGROUNDThe demand for food‐based anti‐photoaging products is surging because of the rising recognition of health and beauty, as well as enhanced comprehension of the detrimental impact of ultraviolet (UV) radiation. This study aimed to investigate the potential of bioactive peptides derived from bovine elastin, specifically focusing on identifying novel elastase inhibitory peptides and assessing their photoprotective properties using bioinformatics techniques.RESULTSA total of 48 bioactive peptides were screened in bovine elastin hydrolysate (EH) utilizing Peptide Ranker analysis. Three novel elastase inhibitory peptides, GAGQPFPI, FFPGAG and FPGIG (in descending order of activity), exhibited potent inhibitory effects on elastase in vitro, surpassing the inhibitory effect of EH by a factor of 1–2 and reaching significantly lower concentrations (8–15 times lower) than EH. The cumulative inhibitory effect of GAGQPFPI, FFPGAG, and FPGIG reached 91.5%. Further analysis revealed that FFPGAG and FPGIG exhibited mixed inhibition, whereas GAGQPFPI displayed non‐competitive inhibition. Molecular simulations showed that these peptides interacted effectively with the elastase active site through hydrogen bonding and hydrophobic interactions. Furthermore, GAGQPFPI, FFPGAG, and FPGIG demonstrated high stability in gastrointestinal digestion, demonstrated transcellular permeability across Caco‐2 cell monolayers, and exhibited remarkable photoprotective properties against UVB‐irradiated HaCaT cells.CONCLUSIONGAGQPFPI showed the most promising potential as a functional food with photoprotective effects against UVB damage and inhibitory properties against elastase. © 2023 Society of Chemical Industry.
Fermentation, functional analysis, and biological activities of turmeric kombucha
Authors: Khazi Mahammed Ilyas , Liaqat Fakhra , Liu Xingrong , Yan Yilin , Zhu Daochen 
Published: September 2023

AbstractBACKGROUNDKombucha is a popular fermented drink with therapeutic benefits. The present study aimed to examine the fermentation of turmeric‐infused kombucha and evaluate its biological activities and functional properties.RESULTSThe study of pH dynamics during fermentation found that turmeric kombucha has a lower pH decrease than standard kombucha, with the lowest pH of 3.1 being observed in 0.1% turmeric kombucha and the maximum pH of 3.8 found in 1% turmeric kombucha. The research shows that the symbiotic consortia of bacteria and yeast alters during the fermentation process with turmeric. Gas chromatogrphy‐mass spectrometry analysis revealed that turmeric kombucha is abundant in terpenes, ketones, alcohols, aldehydes, phenols and fatty acids, with higher levels of active ingredients than regular kombucha. The kombucha with 0.6% turmeric had the highest overall acceptance score (9.0) in sensory evaluation. The total phenolic content after fermentation was in the range 0.2–0.8 mg gallic acid equivalents mL−1. Increasing turmeric concentrations increased the antioxidant, cytotoxic and antibacterial activity of kombucha analogs, with the highest antioxidant activity (89%) observed at 0.8% turmeric, and the maximum cytotoxicity (74%) and antibacterial activity (zones of inhibition of 17.7 and 15.9 mm against Staphylococcus aureus and Escherichia coli, respectively) observed at 1% turmeric.CONCLUSIONThe fermentation of kombucha infused with turmeric enhanced its biological activities, making it a healthier alternative to traditional kombucha and presenting new opportunities in the field of functional foods. Further investigations into the mechanisms underlying these effects and in vivo studies are warranted to fully comprehend the impact of turmeric kombucha consumption on human health. © 2023 Society of Chemical Industry.
Influences of two transport strategies on AMPK‐mediated metabolism and flesh quality of shrimp (Litopenaeus vannamei)
Authors: Guan Weiliang , Nong Wenqian , Wei Xiaobo , Chen Renchi , Huang Zhihai , Ding Yuting , Qin Xiaoming , Cai Luyun , Mao Linchun 
Published: September 2023

AbstractBACKGROUNDWater‐free transportation (WFT), as a novel strategy for express delivery of live shrimp (Litopenaeus vannamei), was developed recently. However, air exposure during this transportation arouses a series of abiotic stress to the shrimp. In the present study, the influences of WFT stress on glycolysis and lipolysis metabolism and meat quality (umami flavor and drip loss) were investigated in comparison with conventional water transportation (WT).RESULTSThe results showed that type II muscle fibers with the feature of anaerobic metabolism were dominated in shrimp flesh. In addition, the increments of intracellular Ca2+ was detected in WFT and WT, which then activated the AMP‐activated protein kinase pathway and promoted the consumption of glycogen, as well as the accumulation of lactate and lipolysis, under the enzymolysis of hexokinase, pyruvate kinase, lactate dehydrogenase and adipose triglyceride lipase. Glycogen glycolyzed to latate. Meanwhile, ATP degraded along with glycolysis resulting in the generation of ATP‐related adenosine phosphates such as inosine monophosphate with umami flavor and phosphoric acid. More remarkable (P < 0.05) physiological changes (except lactate dehydrogenase and lactate) were observed in WFT compared to WT. Additionally, the fatty acid profile also slightly changed.CONCLUSIONThe transport stress induced significant energy metabolism changes of shrimp flesh and therefore effected the flesh quality. The intensifications of freshness (K‐value) of shrimp flesh were detected as a result of ATP degradation, which were more pronounced after WFT. However, the drip loss of shrimp flesh was more significantly increased (P < 0.05) after WFT compared to WT. © 2023 Society of Chemical Industry.
Phytochemical profiles, antioxidant activities, and synergistic antiproliferative effects of blueberry and apple peel extracts
Authors: Xia Wen , Song Bingbing , Li Tong , Liu Rui Hai 
Published: September 2023

AbstractBACKGROUNDBlueberries and apples exhibit favorable bioactivity and health benefits as a result of their rich phytochemicals. Natural phytochemicals exist in complex forms, but there are few reports on whether have additive, synergistic or antagonistic effects between different phytochemicals. The present study aimed to elucidate the synergistic effects of blueberry extract (BE) and apple peel extract (APE) together with respect to inhibiting the proliferation of HepG2 liver cancer cells. Meanwhile, phytochemical characterization of BE and APE was conducted by HPLC, and total antioxidant activity was determined via a cellular antioxidant activity assay, oxygen radical absorption capacity assay and peroxy radical scavenging capacity assay.RESULTSThe results showed that BE and APE were rich in phytochemicals and had potent antioxidant activities, which synergistically inhibited cell proliferation. In the bilateral combination, the dose reduction index value increased by two‐fold, and the combination index value at 95% inhibition was less than 1. Additionally, BE + APE supplementation could promote the expression levels of p53 and c‐myc genes. In conclusion, the BE and APE had strong antioxidant activity and exhibited synergistic inhibition against proliferation of HepG2 cells.CONCLUSIONThe present study can provide a theoretical basis for the synergistic effect of different phytochemicals in health care. © 2023 Society of Chemical Industry.
Accelerometry May be Superior to EMG for Early Evaluation of Vocal Cord Function After Nerve Injury in a Pig Model
Authors: Setså E. , Svendsen Ø.S. , Henriksen B. , Stangeland L. , Husby P. , Brauckhoff K. 
Published: September 2023

ObjectiveVocal cord (VC) movement has been demonstrated by the use of accelerometry (ACC) to decrease in parallel with the electromyographic amplitude (EMG) during ongoing traction injury to the recurrent laryngeal nerve (RLN). When RLN function recovers, discrepancies between EMG and VC movement have been reported in clinical and experimental studies. The present study was conducted to clarify the actual relationship between EMG and VC movement measured by ACC during nerve recovery.MethodsEMG obtained by continuous nerve monitoring (C‐IONM) was compared with ACC during traction injury to the RLN, and throughout 40‐min nerve recovery. A three‐axis linear accelerometer probe was attached to the VC, and ACC data were registered as described. Traction damage was applied to the RLN until there was a 70% amplitude decrease from baseline EMG, or until loss of signal (LOS), that is, EMG values ≤100 μV.ResultsThirty‐two RLN from 16 immature pigs were studied. Correlation between EMG and ACC were calculated during nerve injury and nerve recovery. The mean correlations were for the 70% and LOS group from start to end of traction: 0.82 (±0.17) and 0.87 (±0.17), respectively. Corresponding correlation coefficients during 40‐min recovery was 0.50 (±0.48) in the 70% group and 0.53 (±0.33) in the LOS group.ConclusionThere is a high correlation between EMG and VC movement during nerve injury, and a moderate correlation during early nerve recovery. EMG recovery after RLN injury ensures sufficient VC function as assessed by ACC.Level of EvidenceN/A Laryngoscope, 134:1485–1491, 2024
MuRF‐1 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G‐Actin Ubiquitination
Authors: Wang Hong , Wang Haizhou , Li Xueyan , Xu Wen 
Published: September 2023

ObjectiveMuscle RING‐finger protein‐1 (MuRF‐1), an E3 ubiquitin ligase, has been reported to aggravate skeletal muscle denervated atrophy by mediating the ubiquitination degradation of multiple proteins, whereas the molecular mechanism underlying MuRF‐1‐mediated internal laryngeal muscle denervated atrophy remains unknown.MethodsA rat unilateral recurrent laryngeal nerve (RLN) transection model was established to evaluate denervated muscle atrophy of the larynx. The expression of MuRF‐1, G‐ and F‐actin in thyroarytenoid muscle (TA) myocytes before and after RLN injury was analyzed by immunofluorescence and Western blotting. Coimmunoprecipitation experiments detected molecular interactions between MuRF‐1 and G‐actin. Immunoprecipitation tested MuRF‐1‐mediated ubiquitination of G‐actin in denervated and innervated TA muscle tissues. The shRNA‐MuRF‐1 AAV was used to suppress MuRF‐1 expression in denervated TA muscles in vivo.ResultsFirst, MuRF‐1 expression was significantly elevated in denervated TA muscle compared to innervated TA muscle (p < 0.001). Second, there was a progressive increase in the G/F‐actin ratio in TA myocytes from day 3 to 14 after RLNI (p < 0.01). Furthermore, colocalization of MuRF‐1 and G‐actin in denervated TA myocytes was observed. Moreover, the upregulation of MuRF‐1 was closely associated with the ubiquitination of G‐actin in denervated TA myocytes and muscle tissues. Knockdown of MuRF‐1 decelerated the degree of TA muscle atrophy compared with that in the Blank and NC groups (p < 0.001) but seemed to promote the compensatory movement of the healthy side.ConclusionCollectively, we illustrate a novel molecular mechanism underlying MuRF‐1‐mediated internal laryngeal muscle denervated atrophy in that MuRF‐1 could promote disequilibrium of the G/F‐actin ratio by regulating G‐actin ubiquitination.Level of EvidenceNA Laryngoscope, 134:855–864, 2024
Clinical heterogeneity of polish patients with KAT6B–related disorder
Authors: Magdalena Klaniewska , Anna Bolanowska‐Tyszko , Anna Latos‐Bielenska , Aleksandra Jezela‐Stanek , Krzysztof Szczaluba , Malgorzata Krajewska‐Walasek , Elzbieta Ciara , Magdalena Pelc , Dorota Jurkiewicz , Piotr Stawinski , Agnieszka Zubkiewicz‐Kucharska , Małgorzata Rydzanicz , Rafal Ploski , Robert Smigiel 
Published: September 2023

AbstractBackgroundSay‐Barber‐Biesecker‐Young‐Simpson (SBBYSS) variant of Ohdo syndrome is a rare, autosomal dominant and clinically heterogenous disorder, caused by pathogenic variants in the KAT6B gene located on chromosome 10q22.2. KAT6B encodes a highly conserved histone acetyltransferase belonging to the MYST family. Currently, diseases caused by pathogenic variants in KAT6B (KAT6B‐related disorders) comprise two allelic entities: SBBYSS variant of Ohdo syndrome and genitopatellar syndrome (GPS). Increase in the number of cases with overlapping GPS/SBBYSS phenotype which makes it necessary to redefine this group of phenotypes as KAT6B‐related disorders or KAT6B spectrum disorders.Individuals with SBBYSS usually present with facial abnormalities, hypotonia, joint laxity, feeding problems, and long thumbs/great toes. This syndrome also typically involves skeletal problems including patellar hypoplasia/agenesis.MethodsHere we report six SBBYS syndrome patients with the same dysmorphic features but a different course of the disease. One known and five novel KATB6 pathogenic variants were identified by molecular diagnostics using Next Generation Sequencing (NGS).ResultsWe present a detailed phenotypic analysis of six individuals with KAT6B‐related disorders, in whom a heterozygous pathogenic variant in KAT6B gene was found. In all of our patients facial dysmorphism as well as developmental and speech delay were present. Additionally, all but one patients presented with hypotonia, ocular abnormalities and long thumbs. Most of our probands showed blepharophimosis and skeletal (mainly knee) defects. Contrary to previously reported severe patellar defects (hypoplasia/agenesis) anomalies presented by our patients were less severe (dysplasia, habitual dislocation, subluxation) referring to KAT6B‐related disorders.ConclusionWhile most of the anomalies found in our patients comply with SBBYSS criteria, phenotypic differences in our probands support a broader spectrum of the disease phenotype. To establish the range of this spectrum, a detailed analysis of clinical variability among patients with SBBYSS requires further investigation.
Contributions of trauma and economic insecurity to psychological distress in response to the COVID‐19 pandemic
Authors: Abdul‐Ghani Sarah , Lathan Emma C. , Miao Amanda , Gibbons Rebecca , Eghbalzad Leyla , Powers Abigail , Fani Negar 
Published: September 2023

AbstractRacially minoritized women with limited socioeconomic resources are at increased risk for adverse psychological outcomes in response to the coronavirus disease 2019 (COVID‐19) pandemic. Disproportionate rates of trauma exposure and economic insecurity likely heighten risk for these outcomes among socioeconomically vulnerable individuals, but the unique contributions of these factors are poorly understood. As such, we examined trauma and economic factors as predictors of pandemic‐related psychological distress and symptoms. Ninety‐six women recruited for a trauma research study (91.7% Black, Mage = 38.3 years, SDage = 11.8 years) completed measures of trauma exposure, economic insecurity, and several items assessing psychological distress and symptoms related to the COVID‐19 pandemic. We examined concern for mental and physical health impacts of COVID‐19 as well as changes in self‐reported levels of anxiety and anhedonia from the 3 months before the pandemic to the previous 2 weeks. Linear regression analyses were used to assess contributions of trauma exposure and economic insecurity to COVID‐19‐related distress. Childhood maltreatment and lifetime trauma exposure did not predict COVID‐19‐related distress; however, financial concern significantly contributed to concern for the physical health impact of COVID‐19 (B = 0.30, p < 0.05). Food insecurity emerged as the only significant predictor of concern for the mental health impact of COVID‐19 (B = 0.91, p < 0.01). Housing instability was the only significant predictor of COVID‐19‐related increases in anhedonia (B = −0.30, p < 0.05). Economic insecurity, namely, self‐reported financial concern, food insecurity, and housing instability, was related to COVID‐19‐related psychological distress and symptoms in a sample of predominately Black American women living in under‐resourced communities. Findings may help identify populations at risk for COVID‐19‐related psychological distress and symptoms and appropriate interventions, such as expanding access to nutritious food sources and housing support, for minoritized community members.
Zinc Modulates Glutamine Metabolism in T Cells
Authors: Güzel Nergis , Rink Lothar , Fischer Henrike Josephine 
Published: September 2023

ScopeZinc and glutamine are well known to be essential for the function and polarization of immune cells. TH17 cells are more frequently induced during zinc deficiency and cover their energy requirement mainly through glutaminolysis. A dysregulation of TH17 cells can contribute to the development of autoimmune diseases. Both inhibition of glutaminolysis and zinc supplementation suppress experimental autoimmune encephalomyelitis in mice. Therefore, the aim of this study is to investigate whether zinc modulates glutaminolysis in T cells.Methods and resultsCD3/CD28 stimulation and mixed lymphocytes culture are used as in vitro models for T cell activation. Then, the glutaminolysis is investigated on mRNA, protein, and functional level. Zinc deficiency and glutaminase (GLS) inhibition decrease immune responses in vitro. Furthermore, extracellular zinc and glutamine levels both modulate glutaminolysis by changing the expression of glutamine transporters and key enzymes. Intriguingly, zinc directly interferes with the activity of GLS both in a cell free system and in the cytosol.ConclusionBesides T cell subset differentiation, zinc also impacts on the cellular metabolism by inhibiting glutaminolysis. This suggests that zinc deficiency can contribute to the development of autoimmune diseases whereas zinc supplementation can support their therapy.
Perillaldehyde Mitigates Ionizing Radiation‐Induced Intestinal Injury by Inhibiting Ferroptosis via the Nrf2 Signaling Pathway
Authors: Tang Lin‐Feng , Ma Xiaoming , Xie Li‐Wei , Zhou Hao , Yu Jiahua , Wang Zhen‐Xin , Li Ming 
Published: September 2023

ScopeGastrointestinal toxicity is one of the major side effects of abdominopelvic tumor radiotherapy. Studies have shown that perillaldehyde (PAH) has antioxidant, antiinflammatory, antimicrobial activity, and antitumor effects. This study aims to determine whether PAH has radioprotective effects on radiation‐induced intestinal injury and explore the underlying mechanisms.Methods and resultsC57BL/6J mice are gavaged with PAH for 7 days, then exposed to a single dose of 13 Gy X‐ray total abdominal irradiation (TAI). PAH treatment prolongs the survival time, promotes the survival of crypt cells, attenuates radiation‐induced DNA damage, and mitigates intestinal barrier damage in the irradiated mice. PAH also shows radioprotective effects in intestinal crypt organoids and human intestinal epithelial cells (HIEC‐6). PAH‐mediated radioprotection is associated with the upregulation of nuclear factor erythroid‐2 related factor 2 (Nrf2), activation of the antioxidant pathway, and inhibition of ferroptosis. Notably, treatment with the Nrf2 inhibitor ML385 abolishes the protective effects of PAH, indicating that Nrf2 activation is essential for PAH activity.ConclusionPAH inhibits ionizing radiation (IR)‐induced ferroptosis and attenuates intestinal injury after irradiation by activating Nrf2 signaling. Therefore, PAH is a promising therapeutic strategy for IR‐induced intestinal injury.
Combining electromyographic and electrical impedance data sets through machine learning: A study in D2‐mdx and wild‐type mice
Authors: Pandeya Sarbesh , Sanchez Benjamin , Nagy Janice A. , Rutkove Seward B. 
Published: September 2023

AbstractIntroduction/AimsNeedle impedance‐electromyography (iEMG) assesses the active and passive electrical properties of muscles concurrently by using a novel needle with six electrodes, two for EMG and four for electrical impedance myography (EIM). Here, we assessed an approach for combining multifrequency EMG and EIM data via machine learning (ML) to discriminate D2‐mdx muscular dystrophy and wild‐type (WT) mouse skeletal muscle.MethodsiEMG data were obtained from quadriceps of D2‐mdx mice, a muscular dystrophy model, and WT animals. EIM data were collected with the animals under deep anesthesia and EMG data collected under light anesthesia, allowing for limited spontaneous movement. Fourier transformation was performed on the EMG data to provide power spectra that were sampled across the frequency range using three different approaches. Random forest‐based, nested ML was applied to the EIM and EMG data sets separately and then together to assess healthy versus disease category classification using a nested cross‐validation procedure.ResultsData from 20 D2‐mdx and 20 WT limbs were analyzed. EIM data fared better than EMG data in differentiating healthy from disease mice with 93.1% versus 75.6% accuracy, respectively. Combining EIM and EMG data sets yielded similar performance as EIM data alone with 92.2% accuracy.DiscussionWe have demonstrated an ML‐based approach for combining EIM and EMG data obtained with an iEMG needle. While EIM‐EMG in combination fared no better than EIM alone with this data set, the approach used here demonstrates a novel method of combining the two techniques to characterize the full electrical properties of skeletal muscle.