bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Assessment of Crystallinity and Stability in β‐Ketoenamine‐Based Covalent Organic Frameworks
Authors: Ding Jiehua , Guan Xinyu , Chen Xiaohong , Nan Pihan , Qiu Shilun , Fang Qianrong 
Published: September 2023

AbstractThe design and synthesis of covalent organic frameworks (COFs) with high chemical stability pose significant challenges for practical applications. Although a growing number of robust COFs have been developed and employed for a broad scope of applications, the assessment of COF stability has primarily relied on qualitative descriptions, lacking a rational and quantitative assessment. Herein, a novel assessment method is presented that enables visual and quantitative depiction of COF stability. By analyzing the PXRD patterns of chemically stable β‐ketoenamine‐based COFs (KEA‐COFs), two crystallinity‐dependent parameters are identified, the relative intensity (I2θrel) and the relative area (A2θrel) of the main peak (2θ), which are expected to establish a standardized criterion for assessing COF crystallinity. Based on these parameters, the crystalline changes after stability tests can be visually presented, which provides a rational and quantitative assessment of their stability. This study not only demonstrates the remarkable chemical stability of KEA‐COFs, but also provides valuable insights into the quantitative evaluation of COFs’ crystallinity and stability.
Multiparametric Flow Cytometry for Determination of Viability, Caspase 3 and 7 Activity, and Lipid Peroxidation Adduct (4‐Hydroxynonenal) in Equine Spermatozoa
Authors: da Silva Carolina Maria Balão , Cano Francisco E. Martín , Gaitskell‐Phillips Gemma , Vega Fernando J. Peña 
Published: September 2023

AbstractFlow cytometry is a powerful tool for the analysis of cell samples formed of multipopulations, such as spermatozoa. In recent years, multiparametric cytometers have evolved, allowing the study of different cellular characteristics, such as protein expression, DNA analysis, or mitochondrial activity. Whether using traditional fluorescent dyes or fluorophore‐conjugated antibodies, each cell or cellular component is individually stained, the sample is analyzed at high velocities, and then is displayed and interpreted in a dot‐plot. We hereby describe the procedure to perform a multiparametric flow cytometry analysis in equine spermatozoa using three sources of excitation and polychromatic flow cytometry for the detection of 4HNE, a lipid peroxidation adduct (by anti‐4HNE antibody), apoptotic markers (by caspases 3 and 7 activity), and live/dead spermatozoa (by ethidium‐homodimer) excluding the debris with Hoechst 33342 staining and gating. This multiparametric analysis allows the simultaneous detection of different spermatic parameters, providing useful information for the characterization of a seminal sample and fertility estimation. © 2023 Wiley Periodicals LLC.Basic Protocol: Determination of viability, caspase 3 and 7 activity, and 4‐hydroxynonenal in equine spermatozoa by flow cytometry
The synergy impact of external environmental pressures and corporate environmental commitment on innovations in green technology
Authors: Wang Mingyue , Li Yingming , Wang Zitong , Shi Yuling , Zhou Junbi 
Published: September 2023

AbstractThe adoption of innovations in green technology by enterprises is a decision‐making process that is subject to both external environmental pressures and internal factors. Using data from 4549 private companies in China, this study empirically analyzes the independent and joint effects of four forms of external environmental pressure and environmental commitment on green‐process innovation and green‐product innovation, respectively. The results show that the adoption of innovations in green technology by companies is affected by stakeholder pressure and does not change according to the type of green technology. The marginal effects of external environmental pressure on enterprises' innovations in green technology differ; this is reflected not only in the types of green technology innovation, but also in the types of pressure. Corporate environmental commitment positively moderates the relationship between external environmental pressure and green‐process innovation and the relationship between government‐led environmental regulation and green‐product innovation. However, the relationships between the other three external environmental pressures and green‐product innovation are not significant. This means that for different types of green technological innovation, there are variations in the extent of the synergy between external environmental pressures and corporate environmental commitment.
Detection of adeno‐associated viral DNA in equine post‐administration frozen blood and plasma samples after long‐term storage
Authors: Maniego Jillian , Pesko Bogumila , Habershon‐Butcher Jocelyn , Hincks Pamela , Taylor Polly , Stewart Graham , Proudman Christopher , Ryder Edward 
Published: September 2023

AbstractGene doping in horses is a threat to the fairness in sport and has serious implications for animal welfare. To investigate the effect of long‐term storage on the detection of AAV in plasma and whole blood, samples from an administration study using an adeno‐associated virus serotype 6 expressing green fluorescence protein (AAV6‐GFP) were stored at −20°C for 8 months before analysis. The AAV vector was detected in stored plasma samples, following the same detection profile as the fresh plasma samples. The stored blood showed lower overall DNA detection but followed the same detection profile as the plasma samples. This study provides confidence that re‐analysing plasma samples and/or analysing a frozen ‘B’ sample with different matrix such as whole blood after prolonged storage will still result in the detection of gene doping material.
Multinational cost‐effectiveness analysis of empagliflozin for heart failure patients with ejection fraction >40%
Authors: Kolovos Spyros , Bellanca Leana , Groyer Harinala , Rosano Giuseppe M.C. , Solé Alexandra , Gaultney Jennifer , Linden Stephan 
Published: September 2023

AbstractAimsHeart failure is a chronic progressive condition, with considerable burden on patients' quality of life and economic burden for the healthcare systems. Before the approval of empagliflozin, there were no proven effective treatments for patients with heart failure with left ventricular ejection fraction (HF LVEF) > 40%. The aim of this study was to evaluate the cost‐effectiveness of empagliflozin + standard of care (SoC) compared with SoC alone for patients with HF LVEF > 40%, from the perspective of the healthcare systems of the United Kingdom (UK), Spain, and France, and to quantify the healthcare costs for these patients.Methods and resultsA lifetime Markov cohort state‐transition model was developed based on discrete health states defined by Kansas City Cardiomyopathy Questionnaire‐Clinical Summary Score quartiles to track disease severity. Model inputs relied primarily on the EMPEROR‐Preserved trial data or obtained from published literature or country‐specific databases, as well as local guidelines for the requirements for the conduct of the economic evaluation of healthcare technologies. The total lifetime cost of receiving SoC per patient was £10 092, €15 765, and €14 958 in the UK, Spain, and France, respectively, which increased by £1407, €1148, and €1485, respectively, with the addition of empagliflozin to the SoC. Empagliflozin + SoC was associated with significantly reduced number of hospitalization for HF or cardiovascular death compared with SoC alone, which was a key driver offsetting its drug acquisition costs. The incremental cost‐effectiveness ratio per quality‐adjusted life year (QALY) gained was consistently favourable at £14 851, €11 706, and €15 447 in the UK, Spain, and France, respectively. Scenario analysis using the New York Heart Association functional class showed similar results. Probabilistic sensitivity analyses showed more than 50% probability for cost‐effectiveness for a willingness‐to‐pay (WTP) threshold of £/€20 000/QALY for the three countries.ConclusionsEmpagliflozin was found to be the first targeted treatment option that is clinically effective and cost‐effective for patients with HF LVEF > 40%. Prescribing empagliflozin with SoC to patients with HF LVEF > 40% is expected to improve clinical outcomes and patients' quality of life and substantially below accepted WTP threshold for the healthcare systems in the UK, Spain, and France.
A scoring evaluation for the practical introduction of guideline‐directed medical therapy in heart failure patients
Authors: Matsukawa Ryuichi , Okahara Arihide , Tokutome Masaki , Itonaga Junpei , Koga Eiichi , Hara Ayano , Kisanuki Hiroshi , Sada Masashi , Okabe Kousuke , Kawai Shunsuke , Ogawa Kiyohiro , Matsuura Hirohide , Mukai Yasushi 
Published: September 2023

AbstractAimsThe guideline‐directed medical therapy (GDMT) has been recommended for heart failure (HF) with reduced ejection fraction (HFrEF) based on the accumulating clinical evidence. However, it is difficult to implement all the trial‐proven medications for every patient in the real world.Methods and resultsA simple GDMT score was created, according to the combination of GDMT drugs (renin–angiotensin system inhibitors, beta‐blockers, mineralocorticoid receptor antagonists, and sodium–glucose transporter 2 inhibitors) administration and their dosage (0–9 points). Its impact on the prognosis of HF patients was investigated. Admitted HF patients [HFrEF and HF with mildly reduced ejection fraction (HFmrEF), n = 1054] were retrospectively analysed (excluding those with in‐hospital death and dialysis). A simple GDMT score ≥5, but not the number of medications, was significantly associated with a reduction of all‐cause death, HF readmission, and composite outcome (HF readmission and all‐cause death) (P < 0.001). Subgroup analysis showed that almost all groups with a simple GDMT score of 5 or higher had a better prognosis.ConclusionsThe developed simple GDMT score was associated with prognosis in HFrEF and HFmrEF patients. Even if all four drugs cannot be introduced for some reason, a regimen with a simple GDMT score ≥5 may lead to a prognosis in HF patients.
Spatiotemporal Changes in Trace Metal Bioavailability in the Sediment Pore water of a Constructed Wetland Using Passive Pore water Samplers
Authors: Elhaj Baddar Zeinah , Xu Xiaoyu , Spencer Breann 
Published: September 2023

Abstract
Sediments in aquatic systems often act as a major sink for contaminants. Diffusive gradient in thin films (DGTs) and in situ equilibrium dialysis samplers (peepers) are two major in situ pore water sampling devices that overcome the problems associated with conventional pore water sampling methods. In the present study, DGTs and peepers were used to study the spatial and seasonal effects (cool months, October–February; warm months, May–September) on metal bioavailability in the H-02 constructed wetland and the sink versus source role of the sediments by calculating the metal resupply capacity. Data showed similar seasonal trends in metal concentrations using passive samplers, peepers, and DGTs. Pooled Cu and Zn concentrations measured using DGTs were lower in warm months (1.67 ± 1.50 and 2.62 ± 0.68 μg L–1, respectively, p &lt; 0.001) versus in cool months (2.12 ± 0.65 and 5.58 ± 1.33 μg L–1, respectively, p &lt; 0.001; mean ± 95% confidence interval). Sulfate (SO42−) concentrations were significantly (p = 0.0139) lower in warm months (averaged at 0.22 ± 0.05 mg L–1) compared to in cool months (0.16 ± 0.05 mg L–1). The increase in SO42− concentration is an indicator of the lower activity of sulfate-reducing bacteria, which need SO42− during anaerobic respiration, in which SO42− is reduced to sulfide (S2–) that forms insoluble salts with Cu and Zn, which could partially explain the higher bioavailability of these metals in the cool season. Metal resupply capacity of the sediments was mostly &lt;0.2 for Cu and Zn. Taken together, the H0-2 wetland sediments mostly acted as a sink to both Cu and Zn over the course of the present study. Environ Toxicol Chem 2023;42:2726–2736. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Genomic prediction of seasonal forage yield in perennial ryegrass
Authors: Konkolewska Agnieszka , Phang Steffie , Conaghan Patrick , Milbourne Dan , Lawlor Aonghus , Byrne Stephen 
Published: September 2023

AbstractBackgroundGenomic selection has the potential to accelerate genetic gain in perennial ryegrass breeding, provided complex traits such as forage yield can be predicted with sufficient accuracy.MethodsIn this study, we compared modelling approaches and feature selection strategies to evaluate the accuracy of genomic prediction models for seasonal forage yield production.ResultsOverall, model selection had limited impact on predictive ability when using the full data set. For a baseline genomic best linear unbiased prediction model, the highest mean predictive accuracy was obtained for spring grazing (0.78), summer grazing (0.62) and second cut silage (0.56). In terms of feature selection strategies, using uncorrelated single‐nucleotide polymorphisms (SNPs) had no impact on predictive ability, allowing for a potential decrease of the data set dimensions. With a genome‐wide association study, we found a significant SNP marker for spring grazing, located in the genic region annotated as coding for an enzyme responsible for fucosylation of xyloglucans—major components of the plant cell wall. We also presented an approach to increase interpretability of genomic prediction models with the use of Gene Ontology enrichment analysis.ConclusionsApproaches for feature selection will be relevant in development of low‐cost genotyping platforms in support of routine and cost‐effective implementation of genomic selection.
The spectral slope as a marker of excitation/inhibition ratio and cognitive functioning in multiple sclerosis
Authors: Akbarian Fahimeh , Rossi Chiara , Costers Lars , D'hooghe Marie B. , D'haeseleer Miguel , Nagels Guy , Van Schependom Jeroen 
Published: September 2023

AbstractMultiple sclerosis (MS) is a neurodegenerative disease characterized by neuronal and synaptic loss, resulting in an imbalance of excitatory and inhibitory synaptic transmission and potentially cognitive impairment. Current methods for measuring the excitation/inhibition (E/I) ratio are mostly invasive, but recent research combining neurocomputational modeling with measurements of local field potentials has indicated that the slope with which the power spectrum of neuronal activity captured by electro‐ and/or magnetoencephalography rolls off, is a non‐invasive biomarker of the E/I ratio. A steeper roll‐off is associated with a stronger inhibition. This novel method can be applied to assess the E/I ratio in people with multiple sclerosis (pwMS), detect the effect of medication such as benzodiazepines, and explore its utility as a biomarker for cognition. We recruited 44 healthy control subjects and 95 pwMS who underwent resting‐state magnetoencephalographic recordings. The 1/f spectral slope of the neural power spectra was calculated for each subject and for each brain region. As expected, the spectral slope was significantly steeper in pwMS treated with benzodiazepines (BZDs) compared to pwMS not receiving BZDs (p = .01). In the sub‐cohort of pwMS not treated with BZDs, we observed a steeper slope in cognitively impaired pwMS compared to cognitively preserved pwMS (p = .01) and healthy subjects (p = .02). Furthermore, we observed a significant correlation between 1/f spectral slope and verbal and spatial working memory functioning in the brain regions located in the prefrontal and parietal cortex. In this study, we highlighted the value of the spectral slope in MS by quantifying the effect of benzodiazepines and by putting it forward as a potential biomarker of cognitive deficits in pwMS.
A virtual tracer experiment to assess the temporal origin of root water uptake, evaporation, and drainage
Authors: Nasta Paolo , Šimůnek Jirka , Zhou Tiantian , Stumpp Christine , Romano Nunzio 
Published: September 2023

AbstractThe temporal origin of drainage, evaporation, and root water uptake (RWU) are indicators of ecosystem functioning that shed light on how natural and anthropogenic disturbances affect plant resilience and aquifer vulnerability. A virtual tracer experiment was carried out in HYDRUS‐1D using data from a soil lysimeter planted with winter rye in Austria. The RWU (τR), evaporation (τE), and drainage (τD) transit times (τ) were determined by using the actual dispersivity optimized in a prior study. First, τR and τD were compared to RWU (τPT,R) and drainage (τPT,D) advective transit times estimated using the particle tracking algorithm. The τR values were in agreement with τPT,R while large discrepancies were detected when estimating drainage transit times using the two approaches. A sensitivity analysis revealed that dispersivity had a mild, poor, and strong influence on τR, τE, and τD. The longitudinal dispersivity describes how a tracer spreads along the flow paths. The longer the flow paths, the more dispersion affects the tracer transport. On average, water parcels originating from rainfall in the growing season took 18 and 201 days to reach the roots (with RWU being 21% of rainwater) and the soil profile bottom (with drainage being 36% of rainwater), respectively. In contrast, 10% of rainwater that fell in the dormant season became RWU after 264 days, while 79% became drainage after 294 days. The temporal origin of water can be explored in other plots by using the guidelines proposed in this study.
Evaluation of wearable sports rehabilitation medical measurement system based on the internet of things
Authors: Qi Wenwen 
Published: September 2023

AbstractSports rehabilitation medicine uses sports training and rehab treatment to improve physical function recovery after illnesses, injuries, disabilities, or surgeries. It involves restoring muscle strength, joint range of motion, flexibility, and coordination while reducing pain and soreness, and improving cardiopulmonary function. Wearable IoT devices with sensors and wireless communication tech collect and transmit physiological information for analysis and processing. These devices monitor health, provide exercise training, and assist with rehab in daily wear equipment. The Internet of Things technology can provide a more accurate, convenient and personalized rehabilitation treatment method, accelerating the recovery and rehabilitation process of patients. This project would utilize Internet of Things technology to construct a medical measurement system for sports rehabilitation through measurement algorithms for sports rehabilitation data. Through comparative experiments, the accuracy of the exercise rehabilitation data measurement algorithm based on Internet of Things technology in measuring patient body data was studied and tested. After experimental verification, the actual heart rates of the 10 subjects were 76, 80, 79, 83, 72, 81, 77, 71, 72, and 78, respectively. The data measured using Internet of Things based algorithms were 75, 80, 80, 83, 73, 81, 78, 71, 72, and 77, respectively. Based on this, it could be concluded that the measured values of the algorithm were very close to the actual values, indicating that the algorithm had quite accurate heart rate measurement ability. Therefore, the sports rehabilitation medical testing system constructed by applying this algorithm had strong and accurate data measurement performance.
New protocol to affix sample for environmental and high vacuum electron microscopy
Authors: Mugnai Riccardo , Salazar Sander Santos , Maciel Adeilton Pereira , Andrade Daniel da Silva , Santos Arielly de Sousa 
Published: September 2023

AbstractElectron microscopy has become increasingly essential as an investigative research resource in various fields of science. Despite the evolution of the equipment and preparation techniques, little has been published on techniques for affixation and removal of samples on the stubs after analysis. This work aims to illustrate a quick and low‐cost procedure for fixing and removing a sample from the support for analysis by electronic microscopy, applied to crustacean and insect species, as well as other invertebrates. It thus makes it feasible to use specimens or anatomical portions of specimens represented by a few individuals, or designated as paratypes or holotypes in a collection.Research Highlights
This study presents a quick and low‐cost procedure for fixing and removing samples from support for electron microscopy analysis.
This technique makes it possible to use individual specimens or anatomical portions represented in a collection.
Fluidization of the adhesive and its complete removal allow easy recovery and repositioning of samples.
From zero to ossified: Larval skeletal ontogeny of the Neotropical Cichlid fish Cichlasoma dimerus
Authors: Beriotto Agustina C. , Vissio Paula G. , Gisbert Enric , Fernández Ignacio , Álvarez González Carlos A. , Di Yorio María P. , Sallemi Julieta E. , Pérez Sirkin Daniela I. 
Published: September 2023

AbstractThe identification of skeletal elements, the analysis of their developmental sequence, and the time of their appearance during larval development are essential to broaden the knowledge of each fish species and to recognize skeletal abnormalities that may affect further fish performance. Therefore, this study aimed to provide a general description of the development of the entire skeleton highlighting its variability in Cichlasoma dimerus. Larvae of C. dimersus were stained with alcian blue and alizarin red from hatching to 25 days posthatching. Skeletogenesis began with the endoskeletal disk and some cartilage structures from the caudal fin and the splachnocranium, while the first bony structure observed was the cleithrum. When larvae reached the free‐swimming and exogenous feeding stage, mostly bones from the jaws, the branchial arches, and the opercle series evidenced some degree of ossification, suggesting that the ossification sequence of C. dimerus adjusts to physiological demands such as feeding and ventilation. The caudal region was the most variable regarding meristic counts and evidenced higher incidence of bone deformities. In conclusion, this work provides an overview of C. dimerus skeletogenesis and lays the groundwork for further studies on diverse topics, like developmental plasticity, rearing conditions, or phylogenetic relationships.