bluuebunny/crossref_metadata_2025_split huggingface dataset:
Follow up of Pre-motor Symptoms of Parkinson Disease In Adult Patients With Gaucher Disease Type I and Analysis of Their Lysosomal Enzyme Profiles in Cerebrospinal Fluid
Authors: Wilke Matheus Vernet Machado Bressan , POSWAR Fabiano , BORELLI Wyllians Vendramini , Tirelli Kristiane Michelin , RANDON Devora , LOPES Franciele Fátima , PASETTO Fernanda Bender , SEBASTIÃO Fernanda Medeiros , IOP Gabrielle Dineck , FAQUETI Larissa , KUBASKI Francyne , Schuh Artur Francisco Schumacher , GIUGLIANI Roberto , Schwartz Ida 
Published: June 2023

Abstract
Background
Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide. Its classic motor symptoms may be preceded by non-motor symptoms (NMS). Population studies have identified GBA variants as risk factors for idiopathic PD. The increased risk of PD has also been suggested in other Lysosomal Storage Disorders (LSDs).
Objective
To assess the evolution of the prevalence of NMS compatible with PD in a cohort of South Brazilian adult patients with Gaucher Disease (GD) type 1, already evaluated 3 years ago. Cerebrospinal Fluid (CSF) was collected to assess the levels of LSD enzymes (hexosaminidase, beta-glucuronidase) and biomarker of macrophage activation (chitotriosidase, ChT), compared to controls (metachromatic leukodystrophy, MLD). Cognition was evaluated by the MoCa questionnaire, daytime sleepiness by the Epworth Scale (ESS), depression by Beck´s Inventory, constipation by the UMSARS scale, and REM sleep behavior disorder by the single-question screen. Hyposmia was assessed with Sniffin’ Sticks (SST).
Results
Nineteen patients completed the follow up (mean age of the sample was 44 years; range, 26–71). The patient with the highest number of NMS at the baseline (4 including the lowest SST score) was diagnosed with PD four years later. Apart from an improvement in the ESS score, no other statistical significance was found between the number of NMS between the first and second evaluation, nor between patients with one L444P variant (n = 5) and the rest of the cohort. CSF was collected in five patients (mean age of the sample was 40 years, range 30–53. Significant difference was found between mean CSF activity of total hexosaminidase and beta glucuronidase between GD1 and MLD patients. Mean ChT (CSF) was 62 nmol/h/mL in GD patients and 142 in MLD (n = 7) patients.
Conclusions
The patient with the highest number of NMS in our 2018 cohort was the one that developed PD, corroborating with the importance of this longitudinal follow-up. CSF and plasma analysis might allow a better understanding of the neurodegenerative processes connecting PD and the lysosomal environment. Further analysis is needed to understand this relationship.
M2 TAM-related risk signature based on single-cell and bulk RNA sequencing to evaluate tumor immune microenvironment and predict prognosis in glioma
Authors: Dinghao Zheng , Liming Liu , Chengde Pan , Minghua Tao , Mingshan Tang 
Published: June 2023

Abstract
Background
Glioma is the most common malignant brain tumor, and the treatment effect is still not satisfactory. The tumor microenvironment (TME) is crucial in the incidence and development of tumors. Previous research has shown that TAMs are a vital ingredient of the tumor microenvironment and relate to tumor development, but their roles in glioma remain a mystery.
Results
In this study, we combined the TCGA dataset and GEO single-cell dataset to obtain 58 M2 TAM-related genes. Use univariate Cox regression analysis and LASSO-Cox regression analysis to screen out ten prognostic-related genes and construct a prognostic signature. The CCGA dataset was used to validate the prognostic signature. TCGA and CCGA cohorts were divided into two groups based on the prognostic signature. The AUCs at 1, 3, and 5 years in the TCGA cohort were 0.81, 0.91 and0.90, and 0.67, 0.72 and 0.77 in the CCGA cohort, respectively. In addition, we developed a highly reliable nomogram based on prognostic signatures and clinical characteristics. According to Functional Enrichment Analysis, the differential genes between the two groups were enriched in immune-related pathways. The tumor immune microenvironment showed immune cell infiltration and immune responses are more pronounced in the high-risk group. Drug sensitivity prediction identifies twelve drugs sensitive to high-risk groups, with Bortezomib having the lowest IC50.
Conclusion
In summary, M2 TAM-related risk signature will assist clinical prognosis prediction and personalized treatment of glioma patients.
Diversity, Origin and Evolution of the ESCRT Systems
Authors: Makarova Kira , Tobiasson Victor , Wolf Yuri , Lu Zhongyi , Liu Yang , Zhang Siyu , Krupovic Mart , Li Meng , Koonin Eugene 
Published: June 2023

Abstract
ESCRT (Endosomal Sorting Complexes Required for Transport) is a multicomponent system that plays a key role in protein sorting between membrane-bounded compartments of eukaryotic cells. Homologs of many ESCRT components are identifiable in various groups of archaea but not in bacteria. We performed a comprehensive search for ESCRT protein homologs in archaea and attempted to reconstruct ESCRT evolution using as a scaffold the phylogenetic tree of Vps4 ATPase (ESCRT IV). This reconstruction suggests that the Last Archaeal Common Ancestor possessed a complex ESCRT system that was involved in protein sorting. Subsequent evolution involved either simplification, as in the TACK superphylum, where ESCRT was co-opted for cell division, or complexification as in Asgardarchaeota, the archaeal ancestors of eukaryotes. In Asgard archaea, distinct ESCRT components evolved by gene or domain duplication and recruitment of additional domains, and connection between ESCRT and the ubiquitin system previously considered a eukaryotic signature was established.
Trends in the growth of aggregated measures of failures in a steel conveyor belts core
Authors: Jurdziak Leszek , Błażej Ryszard , Rzeszowska Aleksandra , Kirjanów-Błażej Agata 
Published: June 2023

Abstract
The initial set of four measurements captures the failures occurring in the core of steel link conveyor belt segments used in a lignite coal mine in Poland. These measurements utilize the DiagBelt+ magnetic system, enabling the analysis of averaged damage sizes. Specifically, the sizes of digital damage signals in a 2D image of the belt are examined, with "x" representing the size along the belt axis and "y" representing the size across the belt (measured in the number of channels detecting damage times 25 mm).
The loop of the 2021-meter-long conveyor belt consisted of 11 segments of different ages. Measurements were conducted four times over a span of less than 2 years, allowing for the observation of averaged size changes for a maximum of 6 different time intervals ranging from 1 to 19 months (approximately 1, 6, 7, 12, 13, and 19 months). Due to the replacement of several segments during the analyzed period, five segments were examined only three times.
Conducting measurements on the same conveyor allowed for the examination of changes in the number and sizes of failures while maintaining the stability of all factors that could influence the rate of damage development. The type of material being conveyed (overburden), belt speed (6 m/s), and conveyor length (approximately 1 km) remained constant. The primary factor causing changes in the number and sizes of damages was, therefore, the duration of belt operation between measurements.
The aim of the research was to determine the nature of cumulative increases in length and width of failures in segments over time between measurements. It was found that these increases could be described by linear functions, but their growth rates (expressed by the slope coefficients of the lines) depended on the age of the belt at the time of the first measurement. A regression function was determined for the slope coefficients Ax and Ay in relation to the belt's age. The obtained results are of significant importance for the possibility of developing a reliable short-term forecast of the rate of damage changes in the following months based on the latest measurement. This allows for rational management of the belts, including planned replacement of segments based on their condition, preparation of a belt demand schedule, and aligning the budget with replacement needs.
Quantitative imaging of doxorubicin diffusion and cellular uptake in biomimetic gels with human liver tumor cells
Authors: Degerstedt Oliver , O’Callaghan Paul , Clavero Ada Lerma , Gråsjö Johan , Eriksson Olle , Sjögren Erik , Hansson Per , Heindryckx Femke , Kreuger Johan , Lennernäs Hans 
Published: June 2023

Abstract
Novel tumor-on-a-chip approaches are increasingly used to investigate tumor progression and potential treatment options. To improve the effect of any cancer treatment it is important to have an in depth understanding of drug diffusion, penetration through the tumor extracellular matrix and cellular uptake. In this study, we have developed a miniaturized chip where drug diffusion and cellular uptake in different hydrogel environments can be quantified at high resolution using live imaging. Diffusion of doxorubicin was reduced in a biomimetic hydrogel mimicking tissue properties of cirrhotic liver and early stage hepatocellular carcinoma (373 ± 108 µm2/s) as compared to an agarose gel (501 ± 77 µm2/s, p = 0.019). The diffusion was further lowered to 256 ± 30 µm2/s (p = 0.028) by preparing the biomimetic gel in cell media instead of phosphate buffered saline. The addition of liver tumor cells (Huh7 or HepG2) to the gel, at two different densities, did not significantly influence drug diffusion. Clinically relevant and quantifiable doxorubicin concentration gradients (1–20 µM) were established in the chip within one hour. Intracellular increases in doxorubicin fluorescence correlated with decreasing fluorescence of the DNA-binding stain Hoechst 33342, and based on the quantified intracellular uptake of doxorubicin an apparent cell permeability (9.00 ± 0.74 x 10− 4 µm/s for HepG2) was determined. Finally, the data derived from the in vitro model were applied to a spatio-temporal tissue concentration model to evaluate the potential clinical impact of a cirrhotic extracellular matrix on doxorubicin diffusion and tumor cell uptake.
Risk factors associated with pancreatic fistulas after pancreatoduodenectomy: development and internal validation of a preoperative nomogram
Authors: Namur Guilherme , Mazzucato Fernanda , Jureidini Ricardo , Ribeiro Thiago , Figueira Estela , Rocha Manoel , Ribeiro Ulysses 
Published: June 2023

Abstract
Background
Most complications after pancreatoduodenectomy (PD) are due to pancreatic fistula (PF). Therefore, predicting PF is important to individualize treatment. The aim of this study is to develop a preoperative nomogram to predict PF after PD.
Methods
From January 2009 to January 2018, patients that underwent PD were reviewed. After univariate analyses, variables with p < 0.1 were selected for a multivariate analysis. Variables with p < 0.05 were then included in the nomogram, which was internally validated in a different set of patients.
Results
Out of 180 patients evaluated, 19.4% experienced PF. Risk factors for PF were male gender (OR = 2.89), higher BMI (OR = 1.14) and pancreatic duct diameter ≤ 3 mm (OR = 3.52). Weight loss greater than 10% was protective against PF (OR = 0.16). Using these four variables, a preoperative nomogram was built. The nomogram performed well in the validation set (n = 82 patients, 25.6% with PF) with an area under the ROC curve of 0.798.
Conclusions
male gender, main pancreatic duct ≤ 3 mm and higher BMI are risk factors for PF, while weight loss is protective for it. With these variables, it was possible to build a nomogram that accurately predicts the occurrence of PF.
Effects of Aromatherapy on Pain, Stress, and Behaviors of Newborns: a Randomized Controlled Trial
Authors: Başdaş Öznur , Özbey Harun , Oğul Gülşah , Güneş Tamer 
Published: June 2023

Abstract
Complementary care applications such as aromatherapy can be used to reduce the negative effects of newborns such as pain and stress. This study was conducted as a randomized controlled double-blind trial to examine the effects of massage and bathing using lavender oil on the pain, stress and behavior of newborns (This clinical trial is registered at www.clinicaltrials.gov with ID number NCT05770999). The sample of the study included 45 term newborns (15 lavender massage groups, 15 lavender bath groups, 15 control groups) with a postnatal age of 1-5 days and a gestational age of 38-42 weeks, who could tolerate enterally administered nutrients, were not connected to a respiratory support device, had no skin disease, had no genetic or chromosomal anomalies, and did not undergo surgical intervention, at least one of the parents gave written consent. Ethics Committee and necessary permissions were obtained for the research, and the ethical guidelines of the Declaration of Helsinki were complied with. Descriptive statistics, chi-square, Repeated Measures ANOVA and One-Way ANOVA tests were used to evaluate the data. It was determined that the ALPS- Neo scores of the newborns in the lavender massage group decreased at the 5th minute after the study compared to the before the study. It was determined that the ABSS scores of the newborns in the lavender massage group decreased at the 5th minute after the study compared to the before the study. It was determined that the mean difference between the ALPS-Neo scores of the newborns in the lavender massage group was higher than the other groups. It can be said that aromatherapy massage with lavender oil is more effective in reducing pain and stress levels and improving behavioral outcomes in newborns than a bath application made by adding lavender oil. It is recommended to encourage the application of aromatherapy massage in newborn clinics, to inform parents and to include more studies on this subject.
Highly chemo/regioselective alkoxycarbonylation of terminal allenes catalyzed by Ru/N-ligand system
Authors: Wang Peng , Shi Huibing , Feng Baolin , Zhao Deming , Wu Haigang 
Published: June 2023

Abstract
The Ru-catalyzed highly selective alkoxycarbonylation of allenes with aliphatic alcohols allow to produce useful α, β-unsaturated esters in good to excellent yields under mild condition (temperature is 70 oC). Efficient regioselectivity could be controlled in the presence of N-ligands while the P-ligands had no influence on the conversion of substrates and the chemo/regioselectivity of target products. By involving N-ligand (4,7-dimethyl-1,10-phenanthroline, N9) in the catalytic system, α, β-unsaturated esters were obtained with high conversion and chemo/regioselectivity (Conversion up to 99 %, chemical selectivity up to 99 %, regioselectivity of branched ester/linear ester up to 89/11).
Tet2-mediated macrophage activation promotes liver regeneration
Authors: Chen Yiyuan , Meng Lijun , Xu Nan , Chen Huan , Wei Xuyong , Lu Di , Wang Shuai , Xu Xiao 
Published: June 2023

Abstract
Objective Macrophages are predominant immune cells that secret IL-6 and HGF to promote liver regeneration after liver resection. Ten-eleven translocation- 2 (Tet2) DNA dioxygenase regulates the secretion of pro-inflammatory factors in macrophages. In this study, we explored the role of Tet2 in macrophages and its function independent of its enzymatic activity in liver regeneration.
Methods A 70% partial hepatectomy mice model was established. Enzyme-linked immunosorbent assays, quantitative reverse transcription-polymerase chain reaction, western blotting, immunofluorescences, and flow cytometry were performed to explore the infiltration and phenotypes of immune cells in mice models. Molecular dynamics simulations were carried out to study the interaction of Tet2 with Stat1.
Results We found Tet2 in macrophages negatively regulates liver regeneration in the partial hepatectomy mice model. In mechanism, Tet2 interacts with Stat1 to inhibit the expressions of proinflammatory factors and suppress liver regeneration. Tet2 inhibitor BC339 attenuated the interaction of Stat1 and Tet2, enhanced Stat1 phosphorylation, and promoted hepatocyte proliferation. Notably, Tet2 also directly affected hepatocyte proliferation, independent of the IL-6-Stat3 signaling pathway.
Conclusion Tet2 in macrophages negatively regulates liver regeneration via interacting with Stat1. Our results suggest that specific targeting Tet2 in macrophages promotes the recovery of liver function after hepatectomy. Keywords Macrophage, Liver regeneration, partial hepatectomy, Tet2.
Association between Rheumatoid Arthritis and Renal Function: A Bidirectional Mendelian Randomization Study
Authors: Zhang Yuheng , Zhao Yuechun , Xu Zhijie , cheng Yongzhong , Sang Zhicheng 
Published: June 2023

Abstract
This article investigates whether there is a causal relationship between Rheumatoid arthritis (RA) and renal function (measured by estimated glomerular filtration rate) using Mendelian randomization method. The appropriate single nucleotide polymorphisms were extracted from the GWAS of RA and eGFR as gene instrumental variables. Bidirectional two-sample MR analyses were conducted using inverse variance weighting, weighted median method, and MR Egger regression. The outcome and exposure populations were both East Asians. After screening, a total of 11 single-nucleotide polymorphisms (SNPs) of genome-wide significance were included as RA instrumental variables. The analysis did not show a significant correlation between RA and eGFR (β = 0.007, P = 0.717 in IVW method). Likewise, using 54 genome-wide significant SNPs as eGFR instrumental variables, no causal relationship between eGFR and RA was found (odds ratio = 0.890, 95% CI: 0.691—1.144, p = 0.363). Methods such as MR-Egger regression and weighted median also support the result that there is no evidence of any causal relationship between RA and eGFR in any direction, except for the IVW method.
Use of selective visceral angiography in surgical strategy planning for celiac artery aneurysm in the celiacomesenteric trunk
Authors: Goto Takasumi , Fujimura Hironobu , Shintani Takashi , Shibuya Takashi , Miyagawa Shigeru 
Published: June 2023

Abstract
Background
The celiacomesenteric trunk (CMT) is a common duct of the celiac artery (CA) and the superior mesenteric artery (SMA) originating from the aorta, which is an uncommon anatomical anomaly of the visceral artery. Because of the variety of visceral circulation in those with CMT, the visceral circulation associated with each branch should be evaluated prior to surgical treatment of visceral artery aneurysm in the CMT.
Case Presentation:
A 64-year-old woman was diagnosed with a celiac artery aneurysm in the CMT. Aneurysmectomy of the aneurysm was performed successfully. On preoperative selective visceral angiography, the CA was seen to bifurcate into the common hepatic and splenic artery. The left gastric artery was directly isolated from the aorta and perfused to the common hepatic and splenic artery through collateral circulation. These findings showed that celiac artery embolization is anatomically feasible, even in cases of celiac artery aneurysm rupture.
Conclusions
Selective visceral angiography can contribute to surgical strategy planning in similar cases.
The significance of palpation of the lowest point of the rib in the posterior axillary line for spinal surgery
Authors: Liu zhengang , Li pengfu , Yang Fan , Li Jia-Yi , Liang Yu-Ling , ZHANG BOYIN 
Published: June 2023

Abstract
Objective: The aim of this study was to identify the feasibility of using palpation of the lowest point of the rib in the posterior axillary line to identify the segment of the spine during spinal surgery.
Methods: Three orthopedic surgeons examined 101 patients and determined, by blind palpation, the lowest point of the ribs that could be reached at the posterior axillary line, placed a radiolucent marker, made a plumb line through this point and intersected it at one point C. From this point, a line was made at an angle of 45° to the plumb line and intersected the posterior midline at another point B, where the markers were placed. A posterior anterior lumbar spine X-ray was then taken. Three orthopedic surgeons then identified the spine segment by radiography and assessed the agreement with the spine segment identified by imaging (kappa coefficient).
Results: Point B is most commonly found in the T12 and T11 vertebrae, with an accuracy rate of 86% (87/101).Point C, most commonly at the L3 vertebral body, was 79% (80/101) accurate. The greatest proportion of these was in the upper third of L3. High consistency of results between right and left side on palpation.
Conclusions: In the prone position, clinicians can use this method as a measurement tool with good reliability for palpating the thoracolumbar vertebrae, but the spinal level estimated by palpation may be inadvertently affected by examiner skill and anatomical variation, as well as by issues of individual variation.
Preliminary measurements of oil paint on paper using a mid-infrared hyperspectral imaging system with a bolometer camera and image interferometer.
Authors: SUGAWARA SHIGERU , Ishimaru Ichiro 
Published: June 2023

Abstract
The research focuses on developing a cutting-edge far-infrared HSI (Hyperspectral imaging) system using a bolometer camera, a unique instrument worldwide. This system has been utilized to conduct test measurements on various samples. While previous measurements with this system successfully captured the transmission of samples and the characteristics of inks and adhesives on aluminum plates, the current study introduces improvements in the instrument and measurement method. These advancements have allowed for measuring oil paints on paper, marking a significant achievement. Specifically, 16 different oil paints (four black, four white, four blue, and four red) were subjected to measurement. A compact far-infrared HSI instrument comprising an imaging interferometer and a bolometer camera was employed. The obtained spectra were compared with those obtained using another FTIR spectrometer. To ensure consistent measurements, the sample was rotated during the process, guaranteeing uniform spectra across the entire field of view of the instrument. The success or failure of each measurement was also determined by analyzing the amplitude of the interference signal in each pixel. The results revealed varying degrees of success among the measured oil paints, with paints exhibiting higher reflectance yielding better results, while paints with lower reflectance posed significant challenges. Furthermore, the success of the measurements was influenced by the orientation of the sample's undulating surface. These results show that oil paints can be measured with the world's first far-infrared HIS system with a bolometer camera.