bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on dynamic perfusion control technology of 3D printed tissue vascular network
Authors: Wang Jun-jie , Zhang Li-ming , Li Song 
Published: May 2023

Abstract
In order to solve the problem that the medium cannot be supplied accurately during the perfusion culture of the vascular network in large-volume 3D printing tissue, a perfusion control technology was studied by designing and building an in vitro perfusion and monitoring platform with feedback regulation. First, a large-volume vascular network of hydrogel material was fabricated by 3D bioprinting technology as the experimental object of perfusion. Then we designed and built the dynamic perfusion device and the monitoring equipment in the perfusion process independently and applied the real-time flow calibration control technology on this basis. Finally, the accuracy of the flow real-time calibration control technology was verified through the perfusion experiment of the large-volume vascular network. The results showed that the error rate between the actual flow rate and the preset flow rate increased gradually with the decrease of the preset flow rate of medium perfusion. When the preset flow is 3 mL/min, the error rate between them is as high as 19.33% without the application of flow calibration control technology. The dynamic perfusion device can reduce the average error rate between the two from 8.1% to 0.4% after applying real-time flow calibration control technology, which proves that the device can cultivate a large-volume vascular network with high-precision perfusion after the application of real-time flow calibration control technology.
Analysis and Multi-target Optimisation of the Characteristics with Stepped Cavity of the Hybrid Bearing
Authors: Shen Yu , Pan Zhenhua , Liu Haijiang 
Published: May 2023

Abstract
In this paper, the peak pressure, stiffness, flow rate, and temperature rise of the oil film in the stepped cavity of the hybrid bearing are investigated. The pressure and flow equations for the central section of the stepped cavity and the axial section of the deep and shallow cavity are derived using the simplified Navier-Stokes equations and the boundary conditions of a liquid bearing. Using the control variable method, the influence of the geometrical parameters of the stepped cavity and the process parameters on the oil film characteristics was simulated, and the correctness of the pressure and flow equations was verified. The results show that: the spindle speed is positively correlated with the peak oil film pressure and flow rate; the oil film thickness is negatively correlated with the peak oil film pressure and positively correlated with the flow rate; the depth and width of the shallow cavity are correlated with the peak oil film pressure, but the correlation with the flow rate is low; the maximum error between the theoretical calculation and the simulation analysis is less than 7.3%. The pressure equation and the flow equation are used to establish the objective function with the maximum oil film stiffness, load-bearing capacity, and minimum temperature rise, and the ideal point evaluation function method is used to do a multi-target optimization design to optimize the ladder cavity and obtain the optimal oil cavity geometric parameters. And an 8.75% increase in peak pressure and a 4.1% reduction in temperature rise for the same process parameters.
Detection of warning signal of particulate emission on the basis of fixed threshold in real-time using a lidar system
Authors: Rutigliano N. , Martellucci L. , Puleio A. , Rossi R. , Wyss I. , Gaudio P. 
Published: May 2023

Abstract
Monitoring pollutant emissions in an urban area is of primary importance in order to guarantee high levels of human health (by reducing the impact of respiratory diseases on urban inhabitants) and good preservation of objects (such as historical sites or buildings). The European Environment Agency (EEA) has established that the maximum concentration of PM2.5 concentration for long-term exposure is 10 μg/m3. By measuring the light that has been backscattered by atmospheric particulates, the lidar technique seems to be a valid method to provide a warning signal when a particulate concentration threshold is exceeded. In addition, the lidar system can also provide information about the distance at which the particulate threshold is exceeded. In this research measurements of traffic-originated particulate in the area near the University of Rome “Tor Vergata” is analysed at different times on a weekday to obtain continuous monitoring of its backscattering coefficient, values of particulate concentration and details about time and distance from the lidar system at which a warning signal is provided. The University of Rome “Tor Vergata” is in an area where traffic is very heavy, so high pollutant concentration values are to be expected.
Design and characterization of AmLi neutron sources for the LZ experiment
Authors: Sazzad A.B.M.R. , Busenitz J. , Piepke A. , Poudel S. , Trewin H. , LeViness A. 
Published: May 2023

Abstract
In this paper we describe the development, testing, and characterization of three
low-emission rate AmLi neutron sources. The sources are used to calibrate the nuclear recoil
response of the LUX-ZEPLIN (LZ) dark matter experiment. The sources' neutron emission rate was
measured using 3He proportional tubes. The sources' gamma emissions were
characterized using a high-purity germanium (HPGe) detector. Source-validated Geant4
Monte Carlo simulations allowed to calibrate the Ge and neutron detectors' responses.
Resveratrol loaded native silk fiber-sericin hydrogel double interpenetrating bioactive wound dressing facilitates full-thickness skin wound healing
Authors: Li Yanwei , Wang Susu , Li Yurong , Zhang Guozheng , Wu Tangfeng , Wei Yongkang , Cao Xinyu , Yan Hui , Liang Peisheng , Yan Zihan , Guo Yanan , Zhang Yeshun 
Published: May 2023

Abstract
Wound repair is challenging for traditional wound dressings. New bioactive dressings need to be developed urgently. Herein, we reported a highly bioactive silk protein wound dressing (SPD) with natural silk fiber-sericin hydrogel interpenetrating double network structure, which combines the dual characteristics of natural silk and sericin hydrogel. Silk fiber scaffolds were secreted directly from silkworms bred by regulating their spinning behaviors. Sericin in SPD is obtained by dissolving silkworm cocoons at high temperature and high pressure, while it remains intact activities to self-assemble a hydrogel. To explore the effect of SPD, we first systematically evaluated its physicochemical properties and biological activities in vitro. The SPD exhibits high porosity, prominent mechanical strength, pH-responsive degradability, and excellent anti-oxidation and cell compatibility. Besides, SPD can load and maintain long-term drug release. Based on the satisfactory performance of SPD in vitro, effective in vivo treatment was achieved in a mouse full-thickness wound model, as demonstrated by a significantly accelerated wound healing process, promote the regeneration of hair follicles and sebaceous glands, increased expression of vascular endothelial growth factor, and reduced inflammation. Further, resveratrol was loaded into SPD to enhance the effects of anti-oxidation and anti-inflammation for wound healing. Our investigation shows that SPD with excellent physicochemical and biological properties applied in a murine full-thickness skin wound model resulted in remarkable and efficient acceleration of healing process, which may inspire the design of new, effective, and safer medical materials for tissue regeneration.
Accounting for fuel in fire danger forecasts: the fire occurrence probability index (FOPI)
Authors: Di Giuseppe Francesca 
Published: May 2023

Abstract
A new fire danger index is proposed to overcome one of the most important limitations of current fire danger metrics. The fire occurrence probability index (FOPI) combines the Canadian fire weather index (FWI) with remote observations of vegetation characteristics to better predict landscape flammability. The FOPI is designed to improve fire danger predictions in all fuel-limited environments where fire is driven by the short-term drying of intermittently-available fuel. The FOPI considerably outperforms the FWI in arid biomes while remaining comparable to the FWI where fuel is abundant.
Asymptotic expansions for field moments of bound states
Authors: Forbes G W , Alonso Miguel A 
Published: May 2023

Abstract
Asymptotic expansions are presented for the moments of bound states in one-dimensional anharmonic potentials. The results are derived by using the stable aggregate of flexible element (SAFE) method and include only the first non-zero wave-related correction to the familiar semi-classical approximation. Application to a couple of widely studied potentials that do not permit closed-form solutions is used to demonstrate surprising accuracy even in cases that are far from any asymptotic limit. We explore the absence of alternate terms in the asymptotic expansions as a way to explain the accuracy of the end results. Those results are expressed as definite integrals with integrands involving the parameter used in the SAFE method to control the extent of the associated elemental field contributions. Importantly, the integrals themselves are shown to be precisely independent of that parameter. Further, although the derivation proceeds by way of an asymptotic expansion for the wavefield that involves the associated classical motion, those entities do not appear in the end results which are expressed in terms of just the potential function and its first four derivatives.
Finger gesture recognition with smart skin technology and deep learning
Authors: Ben-Ari Liron , Ben-Ari Adi , Hermon Cheni , Hanein Yael 
Published: May 2023

Abstract
Finger gesture recognition (FGR) was extensively studied in recent years for a wide range of human-machine interface applications. Surface electromyography (sEMG), in particular, is an attractive, enabling technique in the realm of FGR, and both low and high-density sEMG were previously studied. Despite the clear potential, cumbersome electrode wiring and electronic instrumentation render contemporary sEMG-based finger gestures recognition to be performed under unnatural conditions. Recent developments in smart skin technology provide an opportunity to collect sEMG data in more natural conditions. Here we report on a novel approach based on soft 16 electrode array, a miniature and wireless data acquisition unit and neural network analysis, in order to achieve gesture recognition under natural conditions. FGR accuracy values, as high as 93.1%, were achieved for 8 gestures when the training and test data were from the same session. For the first time, high accuracy values are also reported for training and test data from different sessions for three different hand positions. These results demonstrate an important step towards sEMG based gesture recognition in non-laboratory settings, such as in gaming or Metaverse.
Many-body spin rotation by adiabatic passage in spin-1/2 XXZ chains of ultracold atoms
Authors: Dimitrova Ivana , Flannigan Stuart , Kyung Lee Yoo , Lin Hanzhen , Amato-Grill Jesse , Jepsen Niklas , Čepaitė Ieva , Daley Andrew J , Ketterle Wolfgang 
Published: May 2023

Abstract
Quantum many-body phases offer unique properties and emergent phenomena, making them an active area of research. A promising approach for their experimental realization in model systems is to adiabatically follow the ground state of a quantum Hamiltonian from a product state of isolated particles to one that is strongly-correlated. Such protocols are relevant also more broadly in coherent quantum annealing and adiabatic quantum computing. Here we explore one such protocol in a system of ultracold atoms in an optical lattice. A fully magnetized state is connected to a correlated zero-magnetization state (an xy-ferromagnet) by a many-body spin rotation, realized by sweeping the detuning and power of a microwave field. The efficiency is characterized by applying a reverse sweep with a variable relative phase. We restore up to

50
%

of the original magnetization independent of the relative phase, evidence for the formation of correlations. The protocol is limited by the many-body gap of the final state, which is inversely proportional to system size, and technical noise. Our experimental and theoretical studies highlight the potential and challenges for adiabatic preparation protocols to prepare many-body eigenstates of spin Hamiltonians.
Pulmonary metastasectomy and repeat metastasectomy for colorectal pulmonary metastases: outcomes from the Dutch Lung Cancer Audit for Surgery
Authors: van Dorp Martijn , Wolfhagen Nienke , Torensma Bart , Dickhoff Chris , Kazemier Geert , Heineman David J , Schreurs Wilhelmina H 
Published: May 2023

AbstractBackgroundSurgical resection of recurrent pulmonary metastases in patients with colorectal cancer is an established treatment option; however, the evidence for repeat resection is limited. The aim of this study was to analyse long-term outcomes from the Dutch Lung Cancer Audit for Surgery.MethodsData from the mandatory Dutch Lung Cancer Audit for Surgery were used to analyse all patients after metastasectomy or repeat metastasectomy for colorectal pulmonary metastases from January 2012 to December 2019 in the Netherlands. Kaplan–Meier survival analysis was performed to determine the difference in survival. Multivariable Cox regression analyses were performed to identify predictors of survival.ResultsA total of 1237 patients met the inclusion criteria, of which 127 patients underwent repeat metastasectomy. Five-year overall survival was 53 per cent after pulmonary metastasectomy for colorectal pulmonary metastases and 52 per cent after repeat metastasectomy (P = 0.852). The median follow-up was 42 (range 0–285) months. More patients experienced postoperative complications after repeat metastasectomy compared with the first metastasectomy (18.1 per cent versus 11.6 per cent respectively; P = 0.033). Eastern Cooperative Oncology Group performance status greater than or equal to 1 (HR 1.33, 95 per cent c.i. 1.08 to 1.65; P = 0.008), multiple metastases (HR 1.30, 95 per cent c.i. 1.01 to 1.67; P = 0.038), and bilateral metastases (HR 1.50, 95 per cent c.i. 1.01 to 2.22; P = 0.045) were prognostic factors on multivariable analysis for pulmonary metastasectomy. Diffusing capacity of the lungs for carbon monoxide less than 80 per cent (HR 1.04, 95 per cent c.i. 1.01 to 1.06; P = 0.004) was the only prognostic factor on multivariable analysis for repeat metastasectomy.ConclusionThis study demonstrates that patients with colorectal pulmonary metastases have comparable median and 5-year overall survival rates after primary or recurrent pulmonary metastasectomy. However, repeat metastasectomy has a higher risk of postoperative complications.
Aquaporin-4 and GPRC5B: old and new players in controlling brain oedema
Authors: Passchier Emma M J , Kerst Sven , Brouwers Eelke , Hamilton Eline M C , Bisseling Quinty , Bugiani Marianna , Waisfisz Quinten , Kitchen Philip , Unger Lucas , Breur Marjolein , Hoogterp Leoni , de Vries Sharon I , Abbink Truus E M , Kole Maarten H P , Leurs Rob , Vischer Henry F , Brignone Maria S , Ambrosini Elena , Feillet François , Born Alfred P , Epstein Leon G , Mansvelder Huibert D , Min Rogier , van der Knaap Marjo S 
Published: May 2023

Abstract
Brain oedema is a life-threatening complication of various neurological conditions. Understanding molecular mechanisms of brain volume regulation is critical for therapy development. Unique insight comes from monogenic diseases characterized by chronic brain oedema, of which megalencephalic leukoencephalopathy with subcortical cysts (MLC) is the prototype. Variants in MLC1 or GLIALCAM, encoding proteins involved in astrocyte volume regulation, are the main causes of MLC. In some patients, the genetic cause remains unknown.
We performed genetic studies to identify novel gene variants in MLC patients, diagnosed by clinical and MRI features, without MLC1 or GLIALCAM variants. We determined subcellular localization of the related novel proteins in cells and in human brain tissue. We investigated functional consequences of the newly identified variants on volume regulation pathways using cell volume measurements, biochemical analysis and electrophysiology.
We identified a novel homozygous variant in AQP4, encoding the water channel aquaporin-4, in two siblings, and two de novo heterozygous variants in GPRC5B, encoding the orphan G protein-coupled receptor GPRC5B, in three unrelated patients. The AQP4 variant disrupts membrane localization and thereby channel function. GPRC5B, like MLC1, GlialCAM and aquaporin-4, is expressed in astrocyte endfeet in human brain. Cell volume regulation is disrupted in GPRC5B patient-derived lymphoblasts. GPRC5B functionally interacts with ion channels involved in astrocyte volume regulation.
In conclusion, we identify aquaporin-4 and GPRC5B as old and new players in genetic brain oedema. Our findings shed light on the protein complex involved in astrocyte volume regulation and identify GPRC5B as novel potentially druggable target for treating brain oedema.
An Error Term in the Central Limit Theorem for Sums of Discrete Random Variables
Authors: Dolgopyat Dmitry , Fernando Kasun 
Published: May 2023

Abstract
We consider sums of independent identically distributed random variables whose distributions have $d+1$ atoms. Such distributions never admit an Edgeworth expansion of order $d$, but we show that for almost all parameters the Edgeworth expansion of order $d-1$ is valid and the error of the order $d-1$ Edgeworth expansion is typically of order $n^{-d/2}.$
Juvenile idiopathic epilepsy in Arabian horses is not a single-gene disorder
Authors: Ciosek Julia , Kimes Abigail , Vinardell Tatiana , Miller Donald C , Antczak Douglas F , Brooks Samantha 
Published: May 2023

Abstract
Valued for their temperament, beauty, athletic ability, and exhibition in the show ring, Arabian horses are an important component of the horse industry. Juvenile idiopathic epilepsy (JIE), a seizure disorder, is most often reported in Arabian foals from birth to 6 months of age. Affected foals exhibit tonic–clonic seizures lasting as long as 5 min and risking secondary complications like temporary blindness and disorientation. Some foals outgrow this condition, while others die or suffer lifelong complications if not treated. Previous work suggested a strong genetic component to JIE and proposed JIE to be a single-gene trait. In this work, we conducted a genome wide association study (GWAS) in 60 cases of JIE and 120 genetically matched controls, identifying loci suggesting JIE is not caused by a single locus. Coat color (chestnut, gray) phenotypes were used as positive control traits to assess the efficacy of GWAS in this population. Future work will attempt to future define candidate regions and explore a polygenic mode of inheritance.
Predicting Environmental and Ecological Drivers of Human Population Structure
Authors: Pless Evlyn , Eckburg Anders M , Henn Brenna M 
Published: May 2023

Abstract
Landscape, climate, and culture can all structure human populations, but few existing methods are designed to simultaneously disentangle among a large number of variables in explaining genetic patterns. We developed a machine learning method for identifying the variables which best explain migration rates, as measured by the coalescent-based program MAPS that uses shared identical by descent tracts to infer spatial migration across a region of interest. We applied our method to 30 human populations in eastern Africa with high-density single nucleotide polymorphism array data. The remarkable diversity of ethnicities, languages, and environments in this region offers a unique opportunity to explore the variables that shape migration and genetic structure. We explored more than 20 spatial variables relating to landscape, climate, and presence of tsetse flies. The full model explained ∼40% of the variance in migration rate over the past 56 generations. Precipitation, minimum temperature of the coldest month, and elevation were the variables with the highest impact. Among the three groups of tsetse flies, the most impactful was fusca which transmits livestock trypanosomiasis. We also tested for adaptation to high elevation among Ethiopian populations. We did not identify well-known genes related to high elevation, but we did find signatures of positive selection related to metabolism and disease. We conclude that the environment has influenced the migration and adaptation of human populations in eastern Africa; the remaining variance in structure is likely due in part to cultural or other factors not captured in our model.
Reduction ofParaoxonaseExpression Followed by Inactivation across Independent Semiaquatic Mammals Suggests Stepwise Path to Pseudogenization
Authors: Graham Allie M , Jamison Jerrica M , Bustos Marisol , Cournoyer Charlotte , Michaels Alexa , Presnell Jason S , Richter Rebecca , Crocker Daniel E , Fustukjian Ari , Hunter Margaret E , Rea Lorrie D , Marsillach Judit , Furlong Clement E , Meyer Wynn K , Clark Nathan L 
Published: May 2023

AbstractConvergent adaptation to the same environment by multiple lineages frequently involves rapid evolutionary change at the same genes, implicating these genes as important for environmental adaptation. Such adaptive molecular changes may yield either change or loss of protein function; loss of function can eliminate newly deleterious proteins or reduce energy necessary for protein production. We previously found a striking case of recurrent pseudogenization of the Paraoxonase 1 (Pon1) gene among aquatic mammal lineages—Pon1 became a pseudogene with genetic lesions, such as stop codons and frameshifts, at least four times independently in aquatic and semiaquatic mammals. Here, we assess the landscape and pace of pseudogenization by studying Pon1 sequences, expression levels, and enzymatic activity across four aquatic and semiaquatic mammal lineages: pinnipeds, cetaceans, otters, and beavers. We observe in beavers and pinnipeds an unexpected reduction in expression of Pon3, a paralog with similar expression patterns but different substrate preferences. Ultimately, in all lineages with aquatic/semiaquatic members, we find that preceding any coding-level pseudogenization events in Pon1, there is a drastic decrease in expression, followed by relaxed selection, thus allowing accumulation of disrupting mutations. The recurrent loss of Pon1 function in aquatic/semiaquatic lineages is consistent with a benefit to Pon1 functional loss in aquatic environments. Accordingly, we examine diving and dietary traits across pinniped species as potential driving forces of Pon1 functional loss. We find that loss is best associated with diving activity and likely results from changes in selective pressures associated with hypoxia and hypoxia-induced inflammation.