bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging features of Color Doppler Ultrasound, computed tomography and computed tomography dacryocystography in lacrimal sac space-occupying lesions
Authors: Qian Zhenbin , Yu Bo , Yang Ye , Wei Fang , Dong Jianli , Wei Liqing 
Published: January 2023

Abstract
Purpose: Lacrimal sac space-occupying lesions (SOLs) are rare and sometimes life-threatening disorders, often misdiagnosed in clinical practices. This study aimed to observe the imaging features of Color Doppler ultrasound (CDU) and computed tomography (CT) or computed tomography dacryocystography (CT-DCG) in different types of lacrimal sac SOLs.
Methods: This retrospective case series study included 21 patients with lacrimal sac SOLs who underwent lacrimal sac surgery between January 2018 and March 2022. The imaging features of CDU and CT or CT-DCG in these patients extracted from the examination cloud system were observed and analyzed.
Results: 21 patients with lacrimal sac SOLs completed preoperative CDU and CT or CT-DCG examination. CDU showed abnormal lacrimal sac imaging in all cases of SOLs (21/21, 100%), while CT or CT-DCG showed abnormal imaging in 20 cases (20/21, 95.2%). CDU could detect the blood flow signals in all types of space-occupying lesions except mucocele and mucopeptide concretion. Among them, polyps had characteristic imaging changes on CDU and CT-DCG. The mucoceles and mucopeptide concretions had characteristic imaging changes on CDU, which could provide more information for differential diagnosis.
Conclusions: CDU can observe the morphology and internal blood flow signals of lacrimal sac SOLs. CT or CT-DCG has advantages in observing structural damage around the lacrimal sac mass. Therefore, CDU may be used as a routine examination to exclude lacrimal sac SOLs before DCR in the absence of preoperative CT or CT-DCG.
Variable-order topological insulators
Authors: Yang Yating , Sun Handa , Lu Jiuyang , Huang Xueqin , Deng Weiyin , Liu Zhengyou 
Published: January 2023

Abstract
Topological insulators, either the first-order or the higher-order, experience generally a transition to a trivial phase or a topological one of the same order through the gap closing and reopening procedure. Here amazingly, we report a topological insulator, which switches directly between the first and higher orders, with only varying the hoppings and without breaking the symmetry. The phase transition of the first and higher orders is originated from a competition mechanism between the nearest and second-nearest neighbor interactions. This variable-order topological insulator is implemented in a two-dimensional phononic crystal, and the one-dimensional helical edge states, which signal the first-order phase, and the zero-dimensional corner states, which signal the second-order one, are demonstrated in the simulations and experiments. Our study gives deep insight to the topological states of different orders.
Histological and Immunohistochemical Study on the Effect of Cyanoacrylate Topical Adhesive on Healing of Skin Wound Versus Vicryl Suture Among Rabbits
Authors: Selim Manar A. , Hamed Ghada Mohammed 
Published: January 2023

Abstract
It is recommended that cyanoacrylate tissue glue be used in oral surgery to enhance soft tissue healing and reduce problems (pain, inflammation, and bleeding) related to wound healing. The goal of the study was to examine the healing of skin wounds using vicryl suture (control group) and cyanoacrylate tissue adhesive (study group).The histological results showed that skin specimens of control rabbit stained with hematoxylin and eosin stain two weeks after surgery revealed formation of thin epithelial layer with absence of epithelial ridges associated with degeneration of collagen fibers while the skin of the experimental site revealed increased epithelial thickness with formation of epithelial ridges and increased thickness of collagen fiber.
Skin wounds of control site stained with Masson’s trichrome stain revealed mild positive staining reaction of collagen fibers while skin wounds of the experimental site showed strongly positive staining reaction of collagen fibers for Masson’s trichrome stain.
The immunohistochemical localization of transforming growth factor beta (TGF-b) revealed that examination of skin specimens of control site showed weak positive staining reaction while skin wounds of the experimental site showed strongly positive staining reaction.
Immunohistochemical localization of tumor necrosis factor alpha (TNF-α) showed that examination of skin specimens of control site showed weak to moderate positive staining reaction while skin wounds of the experimental site showed weak positive staining reaction.
Vitamin D and its effects on cell adhesion molecules: A systematic review
Authors: Monteiro Jessyca , Souza Thamiris , Curioni Cintia , Cople-Rodrigues Claudia , Citelli Marta 
Published: January 2023

Abstract
In order to systematically review the effects of vitamin D supplementation on cell adhesion molecules (CAM), we conducted a systematic search in eletronic databases to identify placebo-controlled randomized clinical trials published through August 2022. The guiding question was: “In diseases whose complications lead to vascular dysfunction and thrombus formation, is vitamin D supplementation associated with CAM concentrations?”. Studies investigating CAM in all age groups of both sexes using any type and dose of vitamin D supplements were included. Of 617 initially obtained articles, 9 met the inclusion criteria. The articles were divided based on clinical outcomes: Cardiovascular Disease (CVD), Type II Diabetes Mellitus (DM-II), Metabolic Syndrome (MS) and Chronic Kidney Disease (CKD). Four articles reported reduced serum CAM concentrations (two with CKD, one with MS and another with DM-II). One of these articles had a two-month interval supplementation protocol (300,000 IU), two had a weekly protocol (50,000 IU), and one had a daily supplementation protocol (2,000 IU). Vitamin D seems to modulate vascular physiology, especially in patients with vitamin D deficiency and CKD. However, the findings of this review do not allow defining appropriate dosages and supplementation models to reduce endothelial dysfunction and CAM concentration.
Exosomal circEZH2_005, an intestinal injury biomarker, alleviates intestinal ischemia/reperfusion injury by mediating hnRNPA1/Gprc5a signaling
Authors: Zhang Wenjuan , Zhou Bowei , Yang Xiao , Zhao Jin , Hu Jing Juan , Ding Yuqi , Zhan Shuteng , Yang Yifeng , Chen Jun , Zhang Fu , Zhao Bingcheng , Deng Fan , Lin Ze-Bin , Sun Qi-Shun , Zhang Fangling , Yao Zhiwen , Liu Wei-Feng , Li Cai , Liu Ke-xuan 
Published: January 2023

Abstract
Intestinal ischemia/reperfusion (I/R) injury is a severe clinical condition lacking diagnostic markers and has poorly understood molecular mechanisms. Although plasma exosomal circular RNAs (circRNAs) are valuable biomarkers and therapeutic targets for various diseases, their role in intestinal I/R injury remains unknown. This study screens the expression profile of circRNAs in intestinal tissue exosomes collected from intestinal I/R mice and identified circEZH2_005 as a significantly downregulated exosomal circRNA in the plasma of clinical cardiac surgery patients who developed postoperative intestinal I/R injury. The expression of plasma exosomal circEZH2_005 was negatively correlated with the intestinal injury grade. Notably, exosomal circEZH2_005 displayed a significant diagnostic value for intestinal injury induced by I/R. Mechanistically, circEZH2_005 was highly expressed in intestinal crypt cells. CircEZH2_005 upregulation significantly promoted the proliferation of Lgr5+ stem cells by direct interaction with hnRNPA1 and enhanced Gprc5a stability, thereby alleviating I/R-induced intestinal mucosal damage. Hence, exosomal circEZH2_005 may help intestinal I/R injury diagnosis and treatment.
Integration of eQTL and GWAS analysis uncovers a genetic regulation of natural ionomic variation in Arabidopsis
Authors: Xu Chaoqun , Song Ling-Yu , Zhou Ying , Ma Dong-Na , Ding Qian-Su , Guo Ze-Jun , Li Jing , Song Shi-Wei , Zhang Lu-Dan , Zheng Hai-Lei 
Published: January 2023

Abstract
The functional interpretation of traits associated variants by expression quantitative trait loci (eQTL) analysis is usually performed in bulk tissue samples. While the regulation of gene expression is context-dependent, such as cell-type-specific manner. In this study, we estimated cell type abundances from 728 bulk tissue samples using single-cell RNA-sequencing dataset, and performed cis-eQTL mapping to identify cell-type interaction eQTL (cis-eQTLs(ci)) in A. thaliana. Also, we performed Genome-wide association studies (GWAS) analyses for 999 accessions to identify the genetic basis of variations in A. thaliana leaf ionome. As a result, a total of 5,664 unique eQTL genes and 15,038 unique cis-eQTLs(ci) were significant. The majority (62.83%) of cis-eQTLs(ci) were cell-type-specific eQTLs. Using colocalization, we uncovered one interested gene AT2G25590 in Phloem cell, encoding a kind of plant Tudor-like protein with possible chromatin-associated functions, which colocalized with the most significant cis-eQTL(ci) of a Mo-related locus (Chr2:10908806:A:C; P = 3.27×10-27). Furthermore, we prioritized eight target genes associated with AT2G25590, which were previously reported in regulating the concentration of Mo element in A. thaliana. This study revealed the genetic regulation of ionomic variations and provided a foundation for further studies on molecular mechanisms of genetic variants controlling the A. thaliana ionome.
Radiofrequency Ablation for Pediatric Recurrent Hepatocellular Carcinoma: A Single-center Experience
Authors: Long Haiyi , Wu Wenxin , Zhou Luyao , Shen Hui , Xie Xiaoyan , Liu Baoxian 
Published: January 2023

Abstract
Purpose: To summarize our single-center experience with percutaneous ultrasound (US)-guided radiofrequency ablation (RFA) for pediatric recurrent hepatocellular carcinoma (RHCC).
Methods: From September 2007 to September 2021, patients under 18 who underwent percutaneous US-guided RFA for RHCC were retrospectively enrolled in this study. Local effectiveness, complications, local tumor progression (LTP), progression free survival (PFS), and overall survival (OS) were evaluated.
Results: A total of 10 patients (9 male and 1 female; mean age, 11.7±4 years ; age range, 6-17 years) with 15 intrahepatic RHCC lesions were enrolled in this study. Complete ablation (CA) was achieved in 14 out of 15 lesions (93.3%) after the first RFA. During the follow-up (mean, 63.1±18 months; range, 5.3-123.3 months), LTP did not occur. Five patients died including three with tumor progression and one with liver failure. The accumulative one- and three-year PFS rates were 30% and 10%, respectively. The accumulative one- and three-year OS rates were 77.8 % and 44.4%, respectively.
Conclusions: Our single-center experience suggests the safety and feasibility of percutaneous US-guided RFA for pediatric RHCC.
Construction and application of sponge city resilience evaluation system: A case study in Xi 'an, China
Authors: Li Jiake , Jiang Yishuo , zhai Mengmeng , Gao Jiayu , Yao Yutong , Li Yafang 
Published: January 2023

Abstract
Urban vulnerability is evident when highly complex flood risks overlap with diverse cities, and it is important to enhance the resilience of cities to flood shocks. In this study, a sponge city resilience assessment system is established considering engineering, environmental and social indicators, and the grey relational analysis method(GRA) is used to quantify sponge city resilience. At the same time, a multi-objective optimization model is established based on the three dimensions of water ecological environment, drainage safety, and waterlogging safety. The optimal configuration of grey-green infrastructure is weighed by combining the ideal point method, aiming to ensure that cities effectively reduce flood risk through the optimal configuration scheme. Taking the Xiaozhai area in Xi'an as the study area, the evaluation results show that the grey relational degree(GRD)of the resilience indexes of the original scheme is between 0.390–0.661 under the seven different return periods. While the optimization scheme ranges from 0.648–0.765, with the best sponge city resilience at a return period of 2a. Compared with the original scheme, the optimized sponge city resilience level increases from level II to nearly level I under the low return period and from level IV to level II in the high return period, indicating that city's ability to cope with waterlogging and pollution is enhanced significantly. Besides, the main factor affecting the sponge city resilience is the runoff control rate, followed by pollutant load reduction rate, which can provide a methodological framework for the assessment and improvement of sponge city resilience.
ECMO in paediatric cardiac surgery: 5-year single centre experience
Authors: Bezuska Laurynas , O’Doherty Jonathan P. , Ali Bilal , Harvey Chris , Omeje Ikenna , Mimic Branko 
Published: January 2023

Abstract
Background.
Extracorporeal membrane oxygenation (ECMO) has become an integral part of paediatric cardiac surgery. We report the experience of a well-established ECMO service over a 5-year period.
Methods.
This retrospective study analysed all paediatric patients who required ECMO support following cardiac surgery from April 2015 to March 2020. Inclusion criteria were age less than 18 and post-operative ECMO support. Patients were analysed dividing into groups according to the urgency for ECMO support (extracorporeal cardiopulmonary resuscitation (ECPR) and cardiac ECMO) and according to age (neonatal and paediatric ECMO groups). They were followed for 30-day, 6-month mortality, long-term survival, postoperative morbidity and the need for reintervention.
Results.
46 patients were included who had a total of 48 ECMO runs. The 5-year incidence of the need for ECMO after cardiac surgery was 3.3% (48 of the overall 1441 cases recorded). 30-day, 6-month and follow-up survival rate was 85%, 65% and 52% respectively. At the 6-month follow-up, the ECPR group showed a trend towards worse survival compare with the cardiac ECMO group (47% vs 55%) but with no statistical significance (p = 0.35). Furthermore, the survival rates between paediatric (60%) and neonatal (46%) ECMO groups were similar, with no statistical significance (p = 0.45). The rate of acute neurological events was 27% (13/48).
Conclusion.
ECPR and neonatal ECMO groups had higher mortality. ECMO 30-day and 6-month survival rates were better compared with other studies 85% and 65% respectively. Incidence of ECMO and neurological complication rates were in line with other high-volume centres.
Ten-year retrospect of the investigation of proximal limbs metastasis in cancer: A multi-center study on survival outcome, limb function status and surgical procedures analysis
Authors: Zhang Chao , Wang Jun , Wu Haixiao , Lin Yile , Chekhonin Vladimir P. , Peltzer Karl , Bukharov Artem V. , Kaprin Andrey D. , Guo Xu , Liu Zheng 
Published: January 2023

Abstract
Background: The aim of study was to evaluate survival outcome and limb function in cancer patients with proximal limbs metastasis. Associated factors on survival outcome and limb function were identified. The comparative analysis between intramedullary nailing and prosthesis surgery in cancer patients with proximal limb metastasis was performed.
Methods:In this five-center retrospective study, patients diagnosed with limbs metastasis were collected. Descriptive statistics was used and log-rank test was performed to analyze the survival in subgroups. The Cox proportional hazards regression analysis was performed to identify the independent prognostic factors. The Musculoskeletal Tumor Society (MSTS) scoring system was used to evaluate limb function after surgery, and t test or analysis of variance (ANOVA) was utilized in subgroup analysis.
Results:A total of 316 patients with limb metastasis were included with mean age at 61.0 years. The most common primary tumor was breast, followed by renal cancer and lung cancer. The median overall survival was 24.0 months and the 1-, 3- and 5-year survival rates were 86.9%, 34.7% and 6.8%, respectively. Primary tumor type, visceral metastasis and chemotherapy were proved to be the independent prognostic factors. The mean Musculoskeletal Tumor Society (MSTS) score was 20.5, significant difference was observed in subgroup of solitary/multiple bone metastasis, with/without pathological fracture, and type of surgery.
Conclusion:The present study concluded that primary tumor type, visceral metastasis and chemotherapy were three factors affecting the survival of patients. Compared with intramedullary nailing, the patients underwent prosthesis surgery showed better limb function, this procedure should be encouraged in patients with indication.
Resident Perceptions of Competency-Based Korean Medicine Education: A Qualitative, Content Analysis Study Conducted using Focus Group Interviews
Authors: Hong Jiseong 
Published: January 2023

Abstract
Background
The new educational system emphasizes acquisition of clinical competency by the time of graduation from Korean medicine colleges that allow Korean medicine doctors to immediately perform clinical tasks. This study investigated awareness of competency-based education in Korean medicine hospital residents who must simultaneously undergo training and assist medical students in clinical practice.
Methods
This was a qualitative research that was conducted using focus group interviews (FGIs) to investigate the awareness of demands for improvement in competency-based Korean medicine education in Korean medicine hospital. To apply the principles and procedures of FGIs, a semi-structured questionnaire was developed. Data analysis was conducted using the five steps of framework analysis.
Results
According to contents analysis, first competency-based education that reflects actual clinical practice tasks is needed. Second, sufficient basic skill mastery education must be reinforced. Third, an intermediate curriculum that mediates clinical practice and basic education is needed. Fourth, the Objective Structured Clinical Examination and Clinical Performance Examination must be expanded to prepare for the Korean medicine doctor practical test.
Conclusions
Korean medicine residents reported the gap between clinical practice and use of knowledge and skills acquired in the curriculum while acting as direct observers and educations of clinical clerkship in hospitals. Based on this exploratory study it is necessary to conduct research on the educational competency of Korean medicine residents who play an important role as educational leaders in Korean medicine clinical practice training.
The Speed-Gene study: methods, study design and preliminary results
Authors: Htet Swam , Zannah Miftahul , Moe Thet Hnin , Wongveerakul Pongpanot , Charoenpanich Nongnapas , Saengsirisuwan Vitoon , Papadimitriou Ioannis 
Published: January 2023

Abstract
The Speed-Gene study aims to identify genetic variants that influence athletic performance and human locomotion using motion capture technology. Currently 33 participants have already completed the study and the overall aim is to recruit 100 moderately-trained, healthy South-East Asians (all males 18–45 y, BMI < 30). Participants will undergo biomechanical analysis and genetic testing. A number of analyses will be performed, including (but not limited to) linear and angular kinematic analysis using motion capture technology, force plate dynamometry as well as various anthropometric assessments. Pretesting beverages will be provided and activity history as well as current activity levels will be assessed by a questionnaire. The kinematic and force data will be obtained using a Qualisys Track Manager (QTM) system, and DNA will be isolated from white blood cells using standard procedures. The participants will serve as their own controls. Even though the gene Speed-Gene study is tightly controlled, our preliminary findings still indicate considerable individual variability. More participants and further genetic analysis are required to allow us to investigate potential underlying genetic mechanisms responsible for this individual variability.
Association of Socio-economic and Demographic Factors with Incidence of COVID-19 in Nepal
Authors: Shrestha Ruby Maka , Manadhar Ritu , Thapa Janak Kumar , Shakya Prabin Raj 
Published: January 2023

Abstract
Background
The COVID-19 incidence is assumed to have significant impacts on populations with lower socio- economic background across the world. The risk of transmission and spread of COVID-19 disease is higher at cities with higher population density. However, the number of the evidence remains limited in the South Asian region including Nepal. Therefore, the study aimed to understand the COVID-19 disease pattern across Nepal and determine whether the HDI status and population density has an impact on the incidence of disease.
Methods
The study analyzed geographical distributions of cumulative incidence rate and its association with population density and HDI at district level of Nepal. The spatial autocorrelation between cumulative incidence of COVID-19 and HDI and Population Density were measured using bivariate Moran’s I based on Local Indicators of Spatial Association (LISA).
Results
The study analyzed the geographic spread and variance of COVID-19 cases reported from 24 March 2020 till 30 November 2021. The lockdown period was categorized into six stages. The geographical distribution of COVID-19 showed strong spatial autocorrelation with global Moran’s for all six stages. The spatial clusters of COVID-19 incidence were reported in the high population density cities and cities in the southern belt connecting with the Indian border.
Conclusion
Government’s strict prevention and control measures including social restriction and social distance in higher population density areas were helpful to reduce the spread of COVID-19 transmission.
Biliary-enteric reconstruction in laparoscopic radical resection of hilar cholangiocarcinoma: A single-center retrospective cohort study
Authors: Liu Wenzheng , Xiong Fei , Wu Guanhua , Wang Qi , Wang Bing , Chen Yongjun 
Published: January 2023

Abstract
Objective: To evaluate the feasibility and quality of biliary-enteric reconstruction(BER) in laparoscopic radical resection of hilar cholangiocarcinoma (LsRRH) versus open surgery and propose technical recommendations.
Methods: Data of 38 LsRRH and 54 radical laparotomy resections of hilar cholangiocarcinoma (LtRRH) cases were collected from our institution. BERwas evaluated via biliary residuals numbers, number of anastomoses, anastomosis manner, suture method, time consumption, and postoperative complication.
Results: In the LsRRH group, patients were relatively younger; Bismuth type Ⅰ had a higher proportion while type Ⅲa and Ⅳ were less and required no revascularization. In LsRRH and LtRRH groups, respectively, the biliary residuals number was 2.54±1.62 and 2.47±1.46 (p>0.05); the number of anastomoses was 2.04±1.27 and 2.57±1.33 (p>0.05); the time of BER was 65.67±21.53 and 42.5±19.77 min (p< 0.05), 15.08±3.64% and 11.76±2.54% of the total operation time (p<0.05); postoperative bile leakage incidence was 15.79% and 16.67% (p>0.05); 14±10.28 and 17±9.73 days for healing (p<0.05); anastomosis stenosis rate was 2.63% and 1.85% (p>0.05). Neither group had a biliary hemorrhage or bile leakage-related death.
Conclusion: The selection bias in LsRRH mainly affects tumor resection than BER. Our cohort study indicates that BER in LsRRH is technically feasible and equals anastomotic quality to open surgery. However, its longer and a more significant proportion of total operation time represent that BER has higher technical requirements and is one of the critical rate-limiting steps affecting the minimal invasiveness of LsRRH.