bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness and Safety of Vitrectomy Combined with Cataract Surgery Concurrently Versus Vitrectomy with Subsequent Cataract Surgery for Proliferative Diabetic Retinopathy Patients with Mild-moderate Cataract: A Multicenter Randomized Controlled Study
Authors: feng weibo , Zheng Lei , Zhang Guoming , wang xian , li yingqi , Li Yonghao , Liu Bingqian , Jin Ling , huang yinuo , Yang Yangfan , Chen Zidong , Ma Dahui , Chen Qingshan , qin chaojun , feng bingmin , yang zhumin , huang xing , yang chengjie , liu shenghui , Wu Mingxing 
Published: March 2023

Abstract
Purpose To evaluate the effectiveness and safety of pars plana vitrectomy (PPV) combined with phacoemulsification and intraocular lens implantation in proliferative diabetic retinopathy (PDR) patients over 45 years old with mild-moderate cataract, in comparison with PPV with subsequent cataract surgery.
Methods This multi-center randomized controlled trial was conducted in three Chinese hospitals. From February 2021 to October 2021, 168 PDR patients were assessed for eligibility. After exclusion, a total of 129 patients were recruited and randomly assigned to PPV combined with cataract surgery concurrently (the combined group) or PPV with subsequent cataract surgery 6 months later (the subsequent group). A 12-month follow-up period was scheduled. Changes of best correct visual acuity (BCVA), rate of complications were compared between groups.
Results 66 patients were assigned to the combined group and 63 to the subsequent group. The change of BCVA in the combined group with mean 36.90 (95% confidence interval, 95% CI: 30.35-43.45) letters was significantly better (adjusted difference of 16.43, 95% CI: 8.77-24.08, P<0.001) than the subsequent group with 22.40 (95% CI: 15.55-29.24) letters 6 months after the PPV, and no significant difference was found after the cataract surgery in the subsequent group. Compared with the combined group, the rates of neovascular glaucoma (17.65% vs. 3.77%, P=0.005) were significantly higher in the subsequent group.
Conclusion Compared with PPV with subsequent cataract surgery, PPV combined with cataract surgery concurrently was relatively effective, convenient and safe, being a promising and reliable surgical option for PDR patients over 45 years old with mild-moderate cataract.
Trial registration number NCT04729023.
The Prevalence, genetic diversity and evolutionary analysis of Cachavirus firstly detected in northeastern China
Authors: Li Nuawa , Bai Yue , Yan Xin , Guo Zhiyuan , Xiang Kongrui , Yang Zaixing , Shangguan Haikun , Zhao Lili , Ge Junwei 
Published: March 2023

Abstract
Canine Cachavirus was novel parvovirus species which has been firstly detected in dogs in the USA, belonging to the Chaphamaparvovirus genus. However, the prevalence and genetic characteristics of Cachavirus are relatively limited. In this study, 325 canine fecal specimens collected from healthy and diarrheic dogs in northeastern China were screened. A total of 22 out of 325 (6.8%) samples were positive for Cachavirus by PCR. Diarrhea samples showed high viral co-infection rates, and we detected co-infection of canine astrovirus (CaAstV) and Cachavirus for the first time. Moreover, sequence analysis revealed that the Chinese Cachavirus strain has a point mutation at four consecutive amino acid sites compared to the original American strain. And we determined the codon usage bias of the VP1 gene. Codon usage analysis showed that most preferred codons in Cachavirus were A- or T-ended codons, similar to traditional canine parvovirus. Co-evolutionary analysis showed that Cachavirus was likely the result of co-evolution with Feline chaphamaparvovirus. Our findings provide information on the expansion of the limited Cachavirus sequences, point to more details about the molecular characterization of the strains in northeastern China. Further epidemiological surveillance is necessary to determine the significance and evolution of Cachavirus.
3,4-Dihydroxyphenylacetaldehyde synthase evolved an ordered and distinct active site to promote elastic cuticle formation and blood intake in Aedes aegypti
Authors: Chen Jing , Vavricka Christopher , Wei Shuangshuang , Chen Huaqing , Tang Yu , Liang Jing , Hasunuma Tomohisa , Guan Huai , Li Jianyong , Liao Chenghong , Han Qian 
Published: March 2023

Abstract3,4-Dihydroxyphenylacetaldehyde synthase (DHPAAS) catalyzes the direct conversion of 3,4-dihydroxyphenylalanine to 3,4-dihydroxyphenylacetaldehyde (DHPAA), an important intermediate in the formation of flexible insect cuticle. In order to clarify the precise roles DHPAAS plays in insect development and survival, DHPAAS was characterized throughout the physiological to the molecular levels. Extensive in vivo experiments inAedes aegypticonfirm that DHPAAS is essential for blood feeding, egg development and cuticle structure formation. The crystal structure of insect DHPAAS was then solved to reveal the structural basis underlying the catalytic production of the key cuticle intermediate DHPAA. The molecular view shows a DHPAAS active site that is distinct from that of the homologous enzyme 3,4-dihydroxyphenylalanine decarboxylase. Stabilization of the flexible 320–350 region is observed to position the 350–360 loop towards the catalytic asparagine residue, and these distinct features are suggested to promote pyridoxal 5'-phosphate-dependent amine oxidation. Additional molecular dynamics simulations further support the involvement of Phe82, Tyr83 and Asn195 in substrate binding and catalysis, and also shows increased fluctuations limited to loop residues 330–345 inAedes aegyptiDHPAAS.
Simplified Scale for Assessing Behaviors Regarding “Ganbatta” Concept in Children Aged 3–7 Years Undergoing Blood Sampling and Vaccination
Authors: Asari Tsuyoshi , Tabata Hisae , Yamamoto Takeshi , Konno Miki 
Published: March 2023

Abstract
Background

This study aimed to develop a simplified version of the 25-item Ganbatta Scale (i.e., Ganbatta Scale-Short Form to objectively assess behaviors associated with “ganbatta” (“I did my best/I pushed myself/I tried hard”) in children aged 3–7 years who undergo blood sampling and vaccination.

Methods

A cross-sectional survey was conducted among 1143 nurses working in pediatric or outpatient clinics using a questionnaire with 11 items on the respondents' demographics and 25 items on behaviors associated with the “ganbatta” concept in children undergoing needle procedures. Data factor analysis was performed using the principal factor extraction method with promax rotation.

Results

Overall, 3 factors containing 16 items were extracted from the survey results. The three factors were a “Sense of relief and joy after needle removal” (seven items), “Proactive participation” (six items), and “Negative emotional expression” (three items). Reliability analysis revealed coefficients of 0.927 for all 16 items and 0.904, 0.893, and 0.912 for Factors 1, 2, and 3, respectively. We compared the “ganbatta” concept to the three factors; it encompassed various meanings, such as children’s post-procedure sense of joy associated with their successful efforts, as characterized by Factor 1. The children's positive behavior aimed to overcome their existing condition through a change of attitude, as characterized by Factor 2, and their behavior highlighted “perseverance” as a prerequisite skill associated with the “ganbatta” concept, as characterized by Factor 3.

Conclusions

This simplified version of the scale can be used in clinical practice owing to its reduced number of items (16).
Trial registration: Not applicable
Factors Influencing Patient Satisfaction in a Ghanaian Hospital: A Quantitative Study
Authors: Botchwey Charles Owusu-Aduomi , Boateng Agartha Afful , Peprah Mchael 
Published: March 2023

Abstract
Introduction: Patient satisfaction has now become one of the main goals and priorities of many healthcare systems since it is now recognised and accepted as a significant indicator for assessing quality of healthcare, as well as a critical component of performance improvement and clinical effectiveness.
Aim: This study examined the factors influencing patient satisfaction in Ghanaian hospitals,using Trauma and Specialist Hospital in the Effutu Municipality as a case study.
Methodology: The study employed a quantitative approach with a descriptive cross-sectional research design. A well-structured questionnaire, containing close-ended questions was used to collect data from 100 respondents at the Outpatient Department of the Trauma and Specialist Hospital in the Effutu Municipality. Purposive and convenient sampling methods were used to select respondents and data was analysed using Statistical Package for the Social Sciences, Version 20.
Results: The results of the study showed an overall patient satisfaction of 68.97% for the factors assessed, that is, 60.75% for physical environment, 63.75% for accessibility to care and 82.4% for communication. It was also observed that whilst the majority of the respondents were satisfied with most of the dimensions in the three main factors assessed, 53% and 82% of the respondents were dissatisfied with the crowding in the hospital and time spent before seeing the doctor at the Out-Patient Department in the physical environment factor and accessibility factor respectively.
Conclusion: In an industry where mediocrity is given no room, the few deviations from the general trend of the results constitute a clarion call for action by management and relevant stakeholders to further tighten their strategies in their quest to provide an all-inclusive enabling environment to marginally improve upon patient satisfaction in order to fully embrace, activate and realize patient-centred care.
Anatomic study of a nasal septal flap pedicled on the anterior ethmoidal artery for the closure of small anterior nasal septal perforations
Authors: BLANC William , PRADES Jean-michel , KARKAS Alexandre , BERGANDI Florian , HADDAD Ralph , GAVID Marie , LELONGE Yann 
Published: March 2023

Abstract
Objectives: Define endoscopic landmarks through cadaveric dissection for an optimal harvesting of the septal flap, pedicled on the septal branch of the anterior ethmoidal artery, for surgical closure of small anterior septal perforations.
Materials and Method: Twelve nasal cavity dissections were performed on seven human cadavers, after latex injection of the external and internal carotid arteries, respectively in black and red. Twelve mucoperichondrial flaps pedicled on the anterior ethmoidal artery were dissected according to the Castelnuovo method. Macroscopical and microscopical study of the flap’s vascular network were carried out.
Results: The origin of the anterior ethmoidal artery’s septal branch was found at 6.75 ± 2.57 mm posterior to the septal projection of the middle turbinate’s anterior insertion. It had a tangential course 3.67 ± 1.03 mm below the skull base. The nasal floor was exclusively vascularized by the external carotid territory.
Conclusion: The design of the anterior ethmoidal artery pedicled flap is based on a simple and reproducible technique. We recommend its use for small anterior septal perforations closure. The first septal incision should be vertical, close to the posterior edge of the septal perforation followed by another vertical septal incision starting at least 1 cm posteriorly to the septal projection of the middle turbinate’s anterior insertion and extended to the posterior nasal spine of the palatine bone. The vertical incisions should be at least 5 mm below the skull base. The third incision is horizontal, connecting the two previous incisions, without nasal floor mucosa harvesting.
Efficacy and safety of transarterial chemoembolization combined with first-line tyrosine kinase inhibitors and programmed death-1 inhibitors for progressed hepatocellular carcinoma
Authors: Lin Long-Wang , Xie Hang , Ke Kun , Yan Le-Ye , Chen Rong , Lin Jun-Qing , Yang Wei-Zhu 
Published: March 2023

Abstract
Background: After the failure of transarterial chemoembolization (TACE) combined with first-line tyrosine kinase inhibitor therapy, the subsequent therapy for progressed hepatocellular carcinoma (HCC) patients is still controversial. This study was performed to evaluate the safety and efficacy of the subsequent combination of PD-1 inhibitors (TACE combined with first-line tyrosine kinase plus PD-1 inhibitors) relative to switching to the subsequent regorafenib (TACE plus regorafenib).
Methods: The data of HCC patients who suffered from the failure of TACE combined with first-line tyrosine kinase inhibitor therapy from July 2019 to August 2022 were assessed in this single-center retrospective study. Primary study outcomes included progression-free survival (PFS) and overall survival (OS), while the secondary outcomes were treatment-related adverse events, disease control rate (DCR), and objective response rate (ORR).
Results: We enrolled a final total of 113 patients, including 73 patients who received TACE combined with first-line tyrosine kinase and PD-1 inhibitors (Group 1) and 40 patients who received TACE plus regorafenib (Group 2). The OS in Group 1 (15.0; 95% confidence interval [CI], 9.8–20.1 months) was significantly higher compared to Group 2 (9.0; 95% CI, 6.6–11.3 months) (P = 0.016). The PFS in Group 1 (11.0; 95% CI, 8.4–13.5 months) was also significantly higher compared to Group 2 (6.0; 95% CI, 4.6–7.3 months) (P = 0.010). No significant between-group differences in the ORR (P = 0.562) and DCR (P= 0.202) were found; however, the percentage of patients with proteinuria in Group 1 was significantly lower compared to Group 2 (2.73% vs. 20.00%, P= 0.006).
Conclusions: The subsequent combining PD-1 inhibitors after the failure of TACE plus first-line tyrosine kinase inhibitor (TACE combined with first-line tyrosine kinase plus PD-1 inhibitors) may be associated with improved OS and PFS compared with switching to the subsequent regorafenib (TACE plus regorafenib).
Estimation of intrapartum serum vitamin D levels of mother and newborn and its association with newborn anthropometric indices in a tertiary care teaching hospital in Eastern India: An observational study
Authors: Saha Rubina , Kumar Pragya , Ahmad Shamshad , Mahto Mala , Anant Monika , Ranjan Alok , A Anantha Padmanabhan 
Published: March 2023

Abstract
Background
Maternal vitamin D deficiency during pregnancy is a common issue and a significant public health problem at the global level. Foetal growth and development has been over the years studied and it was discovered that it has some linkage with Vitamin D.
Aim & Objective
To estimate vitamin D level of mothers and neonate and find association between serum vitamin D status of mothers and neonate anthropometry.
Materials and Methods
The research was carried out between January 2020 and April 2021. A total of 97 participants—singleton (mother and neonate pair) at the time of delivery—were enrolled using convenient sampling. Five millilitres each of cord blood and maternal venous blood were taken at the time of delivery. Chemiluminescent immunoassay was used to analyse the vitamin D levels. One researcher measured the birth weight, length, head circumference, and chest circumference. Results- Mothers had 87.6% vitamin D deficiency, 5.2% insufficiency and 7.2% sufficiency. About 50.5% of new-borns were vitamin D deficient, 44.3% had insufficient vitamin D levels, and 5.2% had adequate vitamin D levels.
Conclusion
Insufficient vitamin D was reported in 92.8% of mothers. Neonatal vitamin D insufficiency was found to be in 94.8%. Neonatal vitamin D levels were substantially correlated with those of their mothers. Antenatal supplements of calcium along with vitamin D had seen to significantly improve birth weight, Maternal vitamin D levels and the extra supplementation of vitamin D during pregnancy significantly improved neonatal length and head circumference.
Radionuclide therapy based on accumulated urease-powered nanobots in the bladder tumor of an orthotopic murine model
Authors: Simó Cristina , Serra-Casablancas Meritxell , Hortelao Ana , Carlo Valerio Di , Guallar-Garrido Sandra , Plaza-Garcia Sandra , Ramos-Cabrer Pedro , Yagüe Balbino , Aguado Laura , Bardía Lídia , Tosi Sebastien , Gómez-Vallejo Vanessa , Martín Abraham , Patiño Tania , Julián Esther , Colombelli Julien , Llop Jordi , Sanchez Samuel 
Published: March 2023

Abstract
Bladder cancer (BC) is among the most common cancers worldwide. Current treatment methods involving intravesical drug administration present good survival rates but low therapeutic efficacy. Self-propelled nanoparticles (nanobots) could overcome these limitations through their enhanced diffusion and mixing capabilities in urine compared to conventional drugs or passive nanoparticles. Here, we radiolabeled mesoporous silica-based urease-powered nanobots for imaging purposes and tested them in an orthotopic murine model of BC. In vivo and ex vivo results demonstrate enhanced accumulation of nanobots at the tumor site. Positron emission tomography revealed an 8-fold increase in accumulation in vivo. Inductively coupled plasma mass spectrometry confirmed these results. A custom label free optical contrast based on polarization-dependent scattered lightsheet microscopy was applied to cleared bladders to confirm tumor penetration by nanobots. Treating tumor-bearing mice with intravesically administered radio-iodinated nanobots for radionuclide therapy resulted in tumor size reductions of about 90% compared with non-treated mice, positioning nanobots as efficient delivery nanosystems for BC therapy.
Impact of Environmental Temperature on the Pathological Complete Response and Survival Outcomes of Breast Cancer: A NCDB and SEER study
Authors: Gupta Ashish , Gupta Kush , Roy Arya Mariam , Attwood Kristopher , Gandhi Asha , Edge Stephen , Takabe Kazuaki , Repasky Elizabeth , Yao Song , Gandhi Shipra 
Published: March 2023

Abstract
Background: Experimental evidence in tumor-bearing mouse models shows that exposure to cool, that is, sub-thermoneutral environmental temperature is associated with a higher tumor growth rate with an immunosuppressive tumor immune microenvironment than seen at thermoneutral temperatures. However, the translational significance of these findings in humans is unclear. We hypothesized that breast cancer patients living in warmer climates have higher odds of achieving pathologic complete response (pCR) and better survival outcomes than patients living in colder climates.
Methods: A retrospective population-based analysis was conducted on Stage I-III breast cancer patients utilizing data from National Cancer Database (NCDB) from 2010-2018 with 892,092 patients and Surveillance, Epidemiology and End Results (SEER) from 1996-2017 with 270,496 patients. The average annual temperature (AAT) was calculated based on data from the National Centers for Environmental Information.
Results: In the SEER cohort, patients residing at AAT ≥47.5°F had a 16% higher overall survival (OS) (HR 0.84, 95% CI 0.81- 0.88, p <0.001) and 15% higher disease specific survival (DSS) (HR 0.85, 95% CI 0.80 - 0.90; p <0.001). Similarly, 4% higher OS (HR 0.96, 95% CI 0.95-0.97, p <0.001) and DSS (HR 0.96, 95% CI 0.94-0.97, p <0.001) was noted with every 5°F increment in AAT. In the NCDB cohort, patients in regions with AAT ≥ 60.9°F had 9% greater odds of achieving a pCR, odds ratio (OR 1.09, 95% CI 1.05, 1.13, p <0.001) and a 5% higher OS (HR 0.95, 95% CI 0.93 - 0.97, p<0.001).
Conclusions: Higher environmental temperatures are associated with significantly better OS and DSS, as well as higher odds of achieving pCR in these patients. Future research is warranted to confirm this observation using large datasets, to elucidate the underlying mechanisms and investigate novel therapeutic strategies to minimize this geographic disparity in clinical outcomes.
Development of a nanoparticle-assisted triplex RT-PCR assay for porcine reproductive and respiratory syndrome virus detection
Authors: Gao Jie , Wang Hui , Zang Ran , Zhao Zhi-qian , Xu Fei-fei , Zheng Dan-yang , Zhao Mi , Mu Yang 
Published: March 2023

Abstract
Background: Porcine reproductive and respiratory syndrome virus (PRRSV), an enveloped, single-stranded, positive sense RNA virus, has constantly varied and circulated in the pig herd causing severe economic losses worldwide. In this study, a nanoparticle-assisted RT-PCR (Nano-RT-PCR) assay for PRRSV detection was established by the combination of nano-particles with traditional RT-PCR technology.
Results: A rapid detection method based on PRRSV Nsp2 gene sequences to identify classical, highly pathogenic, and NADC30-like strains was developed, and its specificity and sensitivity were evaluated. The results showed that no cross-reaction was observed for the triplex Nano-RT-PCR when detecting related viruses, so as classical swine fever virus (CSFV), porcine epidemic diarrhea virus (PEDV), swine transmissible gastroenteritis virus (TGEV), porcine circovirus type 2 (PCV2), pseudorabies virus (PRV), porcine parvovirus (PPV). The assay was more sensitive than the conventional RT-PCR assay with detection limits of 3.32×102, 6.18×103, and 3.26×103 copies/μL Nsp2 recombinant plasmid of classical, highly pathogenic, and NADC30-like strains which is 10 times, 1 time, and 10 times higher than conventional PCR, respectively. Moreover, the results of 80 clinical samples showed 71.25% were determined PRRSV positive by our Nano RT-PCR assay, compared with 60% for conventional RT-PCR, and that NADC30-like strain had become the main endemic strains of PRRSV in China and there was a mixed infection of two or three strains.
Conclusions: A sensitive and specific Nano-RT-PCR detection method was developed which can be applied in clinical diagnosis and field surveillance of PRRSV infection and determined the strain type.
The effectiveness of a play-based Intervention to develop social skills among Kindergarten children
Authors: Aljunaidi Shaikha , Mokdad Mohamed , Mebarki Bouhafs 
Published: March 2023

Abstract
The current study aimed at verifying the effectiveness of an intervention based on play activities in developing some social skills among kindergarten children. The study used a sample consisted of 40 boys aged (5–6) years who were randomly chosen. For data collection, the study used the kindergarten child social skills assessment scale and the intervention program. Social skills studied in this paper are communication skills, interaction with other skills, cooperation and social participation skills, and expression of feelings skills. Results confirmed the effectiveness of the intervention, which improved the social skills of children. They also indicated the continuity of the effectiveness of the intervention program.
Effect of pepper and cabbage planting on loess of Guyuan region of China on microbial diversity
Authors: Zheng Xuan , Hou Yi , Cheng Guo-Xin 
Published: March 2023

Abstract
Diversity of microorganisms in loess plateau plays critical role in improvement of soil quality. One good strategy is that planting crop improves community of soil microorganisms; however, how microorganism diversity is affected by crop remains unclear. Here, we used a sequencing technology to study the effect of pepper and cabbage crops on soil microorganisms in loess of Guyuan region of China. The analysis revealed that both cabbage and pepper increase the specific Operational Taxonomic Units (OTUs) in soil and diversity of microorganisms was enhanced after cabbage and pepper were cultivated. Ascomycota are the primary fungus species of the soil, and inhibited significantly with the increased soil layers by pepper and cabbage. Contrarily, the development of Actinobacteria is improved and no new microbial species were found with the increased soil layers when pepper and cabbage were planted. (These data indicate that pepper and cabbage promote more reasonable microbial community of the deep soil layer. We speculate that deep ploughing may be a good planting strategy for crop growth in loess plateau. These findings will provide an insight for the improvement of soil quality in the Guyuan region of China.