bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of AUD on death for men with different IQ-scores: a register-based cohort study of 645 955 men
Authors: Honoré Emilie Theisen , Jakobsen Søren Helmer , Osler Merete , Jørgensen Terese Sara Høj 
Published: March 2023

Abstract
Aim: To investigate whether the effect of alcohol use disorder (AUD) on death by natural and unnatural causes, respectively, differs according to intelligence quotient (IQ) scores. Methods: We followed 654 955 Danish men, including 75 267 brothers, born between 1939 and 1959 from their 25th birthday, 1 January 1970, or date of conscription (whichever came last) until 31 December 2018. The exposure of AUD was defined by first registered treatment (diagnosis since 1969, prescription medicine since 1994, or other treatment since 2006), and the outcomes of death by natural and unnatural causes, respectively, were obtained from nationwide registers since 1970. Information on IQ score was retrieved at conscription from the Danish Conscription Database. Results and conclusion: In total, 86 106 men were defined with an AUD. AUD combined with the highest, middle, and lowest IQ score tertiles, respectively, were associated with a 5.90 (95% confidence interval [CI] 5.75; 6.01), 6.88 (95% CI: 6.73; 7.04), and 7.53 (95% CI: 7.38; 7.68) times higher hazard of death by natural causes compared with no AUD and the highest IQ score tertile. The risk of death by unnatural causes was comparable for men with AUD regardless of IQ score tertile. A within-brother analysis showed that the impact of AUD on death by natural and unnatural causes, respectively, did not vary between men with different IQ score tertiles, but were hampered by statistical uncertainty. Our study indicates a need of special focus on men with lower levels of IQ score and AUD for prevention of death by natural causes.
Efficient gene targeting in Aspergillus chevalieri used to produce katsuobushi
Authors: Hiramatsu Kentaro , Nishitani Atsushi , Okutsu Kayu , Yoshizaki Yumiko , Takamine Kazunori , Tamaki Hisanori , Futagami Taiki 
Published: March 2023

ABSTRACT
In this study, we developed an efficient gene targeting system for the osmophilic fungus Aspergillus chevalieri, which is commonly used in the production of a dried bonito, katsuobushi. Specifically, we utilized the clustered regularly interspaced short palindromic repeats/Cas9 system to disrupt the ATP sulfurylase encoding sC gene. This results in methionine auxotroph and selenate-resistance. Additionally, we disrupted the DNA ligase IV encoding ligD gene, which is required for nonhomologous end joining. Using the sC marker and selenate-resistance as a selection pressure, we were able to rescue the sC marker and generate a ΔligD ΔsC strain. We determined that the gene targeting efficiency of the ΔligD ΔsC strain was significantly higher than that of the parental ΔsC strain, which indicates that this strain provides efficient genetic recombination for the genetic analysis of A. chevalieri.
Overcoming barriers to social inclusion in agricultural intensification: reflections on a transdisciplinary community development project from India and Bangladesh
Authors: Roth Christian H , Cosijn Michaela , Carter Lucy , Chakraborty Arnab , Das Mahanambrota , Hamilton Serena H , Kumar Jana Alak , Lim-Camacho Lilly , Majumdar Subrata , Merritt Wendy S , Mishra Pulak , Mishra Rajeshwar , Nidumolu Uday , Rahman Md Wakilur , Ray Dhananjay , Williams Liana J 
Published: March 2023

Abstract
We describe a research-for-development (R4D) strategy developed to address how investments and interventions in agricultural intensification as a means to achieve community development can be designed to be more socially inclusive and equitable. We draw on results from a 5-year project – Promoting socially inclusive and sustainable agricultural intensification in West Bengal (India) and southern Bangladesh (SIAGI). We reflect on a major pivot in the project’s strategy, from being primarily research-driven to placing community concerns and priorities at the centre with a shift towards Ethical Community Engagement (ECE). This became the foundational framework which guided the definition and undertaking of all subsequent activities – including a rethink of methods and concepts to develop tools and frameworks fit for purpose and local context, and inculcating a culture of reflexivity and mutual learning in the project. We show that creating the conditions for true participation, where project beneficiaries and non-government organizations are equal partners alongside researchers and government actors, and for co-learning using the ECE framework, sets the foundations for increased and potentially enduring social inclusion in agricultural intensification.
The neurocognitive effects of atrial fibrillation: benefits of the ABC pathway
Authors: Calvert Peter , Gupta Dhiraj , Lip Gregory Y H 
Published: March 2023

Abstract
Atrial fibrillation (AF) has a recognized association with not only stroke, but also neurocognitive impairment and both vascular and Alzheimer's dementia. Effective management of AF can reduce the risk of such complications. In this narrative review article, we discuss the pathophysiological links between AF and dementia, as well as the benefits of adherence to the guideline-recommended ‘ABC’ pathway.
The SR protein RSP-2 influences expression of the truncated insulin receptor DAF-2B in Caenorhabditis elegans
Authors: Martinez Bryan A , Gill Matthew S 
Published: March 2023

Abstract
The alternatively spliced daf-2b transcript in Caenorhabditis elegans encodes a truncated isoform of the nematode insulin receptor that retains the extracellular ligand binding domain but lacks the intracellular signaling domain and is therefore unable to transduce a signal. To identify factors that influence expression of daf-2b, we performed a targeted RNA interference screen of rsp genes, which encode splicing factors from the serine/arginine protein family. Loss of rsp-2 significantly increased the expression of a fluorescent daf-2b splicing reporter, as well as increasing expression of endogenous daf-2b transcripts. Correspondingly, rsp-2 mutants exhibited similar phenotypes to those previously observed with DAF-2B overexpression, namely suppression of pheromone-induced dauer formation, enhancement of dauer entry in insulin signaling mutants, inhibition of dauer recovery, and increased lifespan. However, the epistatic relationship between rsp-2 and daf-2b varied according to the experimental context. Increased dauer entry and delayed dauer exit of rsp-2 mutants in an insulin signaling mutant background were partially dependent on daf-2b. Conversely, suppression of pheromone-induced dauer formation and increased lifespan in rsp-2 mutants were independent of daf-2b. These data demonstrate that C. elegans RSP-2, an ortholog of human splicing factor protein SRSF5/SRp40, is involved in regulating the expression of the truncated DAF-2B isoform. However, we also find that RSP-2 can influence dauer formation and lifespan independently of DAF-2B.
An improved reference of the grapevine genome reasserts the origin of the PN40024 highly homozygous genotype
Authors: Velt Amandine , Frommer Bianca , Blanc Sophie , Holtgräwe Daniela , Duchêne Éric , Dumas Vincent , Grimplet Jérôme , Hugueney Philippe , Kim Catherine , Lahaye Marie , Matus José Tomás , Navarro-Payá David , Orduña Luis , Tello-Ruiz Marcela K , Vitulo Nicola , Ware Doreen , Rustenholz Camille 
Published: March 2023

Abstract
The genome sequence of the diploid and highly homozygous Vitis vinifera genotype PN40024 serves as the reference for many grapevine studies. Despite several improvements to the PN40024 genome assembly, its current version PN12X.v2 is quite fragmented and only represents the haploid state of the genome with mixed haplotypes. In fact, being nearly homozygous, this genome contains several heterozygous regions that are yet to be resolved. Taking the opportunity of improvements that long-read sequencing technologies offer to fully discriminate haplotype sequences, an improved version of the reference, called PN40024.v4, was generated. Through incorporating long genomic sequencing reads to the assembly, the continuity of the 12X.v2 scaffolds was highly increased with a total number decreasing from 2,059 to 640 and a reduction in N bases of 88%. Additionally, the full alternative haplotype sequence was built for the first time, the chromosome anchoring was improved and the number of unplaced scaffolds was reduced by half. To obtain a high-quality gene annotation that outperforms previous versions, a liftover approach was complemented with an optimized annotation workflow for Vitis. Integration of the gene reference catalogue and its manual curation have also assisted in improving the annotation, while defining the most reliable estimation of 35,230 genes to date. Finally, we demonstrated that PN40024 resulted from 9 selfings of cv. “Helfensteiner” (cross of cv. “Pinot noir” and “Schiava grossa”) instead of a single “Pinot noir”. These advances will help maintain the PN40024 genome as a gold-standard reference, also contributing toward the eventual elaboration of the grapevine pangenome.
Design and validation of a high-density single nucleotide polymorphism array for the Eastern oyster (Crassostrea virginica)
Authors: Xuereb Amanda , Nahuelpi Rodrigo Marín , Normandeau Eric , Babin Charles , Laporte Martin , Mallet André , Yáñez José M , Mallet Martin , Bernatchez Louis 
Published: March 2023

Abstract
Dense single nucleotide polymorphism (SNP) arrays are essential tools for rapid high-throughput genotyping for many genetic analyses, including genomic selection and high-resolution population genomic assessments. We present a high-density (200 K) SNP array developed for the Eastern oyster (Crassostrea virginica), which is a species of significant aquaculture production and restoration efforts throughout its native range. SNP discovery was performed using low-coverage whole-genome sequencing of 435 F1 oysters from families from 11 founder populations in New Brunswick, Canada. An Affymetrix Axiom Custom array was created with 219,447 SNPs meeting stringent selection criteria and validated by genotyping more than 4,000 oysters across 2 generations. In total, 144,570 SNPs had a call rate &gt;90%, most of which (96%) were polymorphic and were distributed across the Eastern oyster reference genome, with similar levels of genetic diversity observed in both generations. Linkage disequilibrium was low (maximum r2 ∼0.32) and decayed moderately with increasing distance between SNP pairs. Taking advantage of our intergenerational data set, we quantified Mendelian inheritance errors to validate SNP selection. Although most of SNPs exhibited low Mendelian inheritance error rates overall, with 72% of called SNPs having an error rate of &lt;1%, many loci had elevated Mendelian inheritance error rates, potentially indicating the presence of null alleles. This SNP panel provides a necessary tool to enable routine application of genomic approaches, including genomic selection, in C. virginica selective breeding programs. As demand for production increases, this resource will be essential for accelerating production and sustaining the Canadian oyster aquaculture industry.
Mainstreaming Marxism: on the anarchic structure of world economy
Authors: Morton Adam David 
Published: March 2023

Abstract
This article carves out a focus on certain authorized mainstream perspectives and their theorizations of world order, and how they have become dominant at the expense of excluded and silenced contributions. This task begins, first, by asserting that the anarchic conditions of world order have been mainstreamed at the expense of contributions to Marxist political economy. Here, my focus extends the methodological approach of juxtaposition to explore competing understandings of anarchic orders in Kenneth Waltz's and Nikolai Bukharin's work to disclose, in the latter, the anarchic structure of world capitalism. Second, the method of juxtaposition enables me to cast attention to the parallel profiles of E. H. Carr and C. L. R. James and their weighty understandings of world revolution to reveal, in the latter, neglected classed conditions of racial capitalism. In a fresh manner, then, my approach juxtaposes key figures that have been present (Waltz, Carr) and absent (Bukharin, James) in understanding world order through the anarchic structure of the world economy and racial capitalism. In conclusion, the argument left for academic study and the elucidation of policy is the extent to which a necessarily historical materialist moment to understanding world order can and should be further extended and deepened.
A Randomized, Double-Blind, Midazolam-Controlled Trial of Low-Dose Ketamine Infusion in Patients With Treatment-Resistant Depression and Prominent Suicidal Ideation
Authors: Su Tung-Ping , Li Cheng-Ta , Lin Wei-Chen , Wu Hui-Ju , Tsai Shih-Jen , Bai Ya-Mei , Mao Wei-Chung , Tu Pei-Chi , Chen Li-Fen , Li Wei-Chi , Chen Mu-Hong 
Published: March 2023

Abstract

Background
The benefits of low-dose ketamine for patients with treatment-resistant depression (TRD) and prominent suicidal ideation require further investigation. The effects of treatment refractoriness, the duration of the current depressive episode, and the number of prior antidepressant failures on ketamine efficacy also require clarification.

Methods
We recruited 84 outpatients with TRD and prominent suicidal ideation—defined as a score ≥4 on item 10 of the Montgomery–Åsberg Depression Rating Scale (MADRS)—and randomized them into 2 groups to receive 0.5 mg/kg ketamine or 0.045 mg/kg midazolam. We assessed depressive and suicidal symptoms prior to infusion; 240 minutes post infusion; and 2, 3, 5, 7, and 14 days post infusion.

Results
According to the MADRS scores, the antidepressant effect (P = .035) was significantly noted in the ketamine group up to 14 days than in the midazolam group. However, the antisuicidal effect of ketamine, as measured by the Columbia-Suicide Severity Rating Scale Ideation Severity Subscale (P = .040) and MADRS item 10 (P = .023), persisted only 5 days post infusion. Furthermore, the antidepressant and antisuicidal effects of ketamine infusion were noted particularly in patients whose current depressive episode lasted &lt;24 months or whose number of failed antidepressants was ≤4.

Conclusions
Low-dose ketamine infusion is a safe, tolerable, and effective treatment for patients with TRD and prominent suicidal ideation. Our study highlights the importance of timing; specifically, ketamine is more likely to achieve therapeutic response when the current depressive episode lasted &lt;24 months and the number of failed antidepressants is ≤4.
Financial strain of COVID-19 and its impact on willingness-to-pay for equine care
Authors: Thompson Jada M , Kibler Michelle L , Ivey Jennie L Z 
Published: March 2023

Abstract
The novel COVID-19 virus caused a global pandemic disrupting lives, industries, and economies. The result was an impact on prices due to challenges with production and supply chain distribution. This study investigates the financial strain COVID-19 had on equine owners and leasers, what the market for equine care would bear if costs for care increased, and what factors contribute to their willingness to pay (WTP) for increasing cost of equine care. An online survey was distributed for 4 weeks to adult U.S. residents. Respondents reported their involvement in the equine industry, financial response to COVID-19, and responses to a double-bound dichotomous choice question on their WTP for care given a randomized increase (1%–20%) in their current cost (n = 506). Data were analyzed using interval regression models where a = 0.05 (Stata15). Respondents were separated into three groups: owner keeping their horse at their residence, owner boarding their equids, and leaser. Boarders reported mean monthly payments of 23.33 ± 90.37 USD (n = 15) for free board, 236.47 ± 151.92 USD, (n = 75) for partial board, and 514.75 ± 291.71 USD (n = 181) for full board. Results show all owners, leaser, and boarders have different WTP values which range from 18.5% to 26.2% increase in current care costs which extends beyond the presented range due to many respondents responding “yes” to both WTP questions (71% of owners, 6% of boarders, 65% of leasers). Equine owners, with on farm equids from the southern US were WTP 11% less than from other regions (P = 0.015). The current boarding fees from owners that board their equids lowered their WTP by 0.01% (P = 0.029) for each additional dollar paid, whereas current care costs were not a significant factor for other owners (P = 0.370) or leasers (P = 0.395). Those that had a full lease for their equids, housed on farm or at a facility, were WTP 15% higher (P = 0.036) than those that had a partial or no lease. In comparison, boarding status (full, partial, or no boarding) did not significantly (P = 0.51) impact boarder’s WTP. Age of respondent and annual household income heterogeneously affected WTP across all groups. These results indicate the market for equine care can bear the increases in cost associated with financial distress related to COVID-19, and may aid equine owners, caregivers, and associated individuals in making informed decisions regarding essential care. Results from this study should be taken in context of the global pandemic and the restrictions in place, or lack thereof, at the time the survey was administered.
Effects of α-lipoic acid on growth performance, antioxidant capacity, and immune function in sheep
Authors: Yang Xinlu , Zhang Hongrui , Pang Fanglin , Zhang Lijie , Fu Tong , Wang Linfeng , Liu Kaizhen , Gao Tengyun 
Published: March 2023

Abstractα-Lipoic acid (α-LA) was usually applied to improve the ability of removing the reactive oxygen species of host. The affection of α-LA on ruminants was mainly focused on the variation of serum antioxidant and immune indexes, but the research on tissues or organs remained limited. The aim of this study was to explore the effects of dietary supplementation with different levels of α-LA on growth performance, antioxidant status, and immune indexes of serum and tissues in sheep. One hundred Duhu F1 hybrid (Dupo♀ × Hu sheep♂) sheep aged 2 to 3 mo with similar body weight (27.49 ± 2.10 kg) were randomly allocated into five groups. Five diets supplemented with 0 (CTL), 300 (LA300), 450 (LA450), 600 (LA600), and 750 (LA750) mg/kg α-LA were fed to sheep for 60 d. The results showed that α-LA supplementation significantly increased the average daily feed intake (P &lt; 0.05); however, no significant variation was found in the average daily gain, feed conversion rate, carcass weight, and slaughter rate among groups (P &gt; 0.05). Compared with CTL group, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in serum of LA600 and LA750 groups were increased (P &lt; 0.05). At LA450–LA750 groups, SOD, CAT activities in liver and ileum tissues and GSH-Px activities in ileum tissues were increased than CTL group (P &lt; 0.05), while malondialdehyde (MDA) contents in serum and muscle tissue were decreased than CTL group (P &lt; 0.05). The total antioxidant capacity contents in liver, muscle, and ileum tissues of LA600 group were increased compared with CTL group (P &lt; 0.05). Additionally, the interleukin-10 (IL-10) contents of serum in LA450–LA750 groups were increased than CTL group (P &lt; 0.05); the contents of interleukin-1β (IL-1β) in serum, interleukin-2 (IL-2) in liver, and interleukin-6, IL-1β in muscle were decreased than CTL group (P &lt; 0.05). The content of immunoglobulin A in serum of LA600 group, ileum, and muscle of LA750 group was increased than CTL group (P &lt; 0.05). Based on the quadratic regression analysis of GSH-Px, MDA, IL-2, IL-10, and IL-1β, the optimal dietary α-LA levels were estimated to be 495.75, 571.43, 679.03, 749.75, and 678.25 mg/kg, respectively. This research will provide certain contribution for the effective utilization of α-LA in sheep production.
An finite element analysis surrogate model with boundary oriented graph embedding approach for rapid design
Authors: Fu Xingyu , Zhou Fengfeng , Peddireddy Dheeraj , Kang Zhengyang , Jun Martin Byung-Guk , Aggarwal Vaneet 
Published: March 2023

Abstract
In this work, we present a boundary oriented graph embedding (BOGE) approach for the graph neural network to assist in rapid design and digital prototyping. The cantilever beam problem has been solved as an example to validate its potential of providing physical field results and optimized designs using only 10 ms. Providing shortcuts for both boundary elements and local neighbor elements, the BOGE approach can embed unstructured mesh elements into the graph and performs an efficient regression on large-scale triangular-mesh-based finite element analysis (FEA) results, which cannot be realized by other machine-learning-based surrogate methods. It has the potential to serve as a surrogate model for other boundary value problems. Focusing on the cantilever beam problem, the BOGE approach with 3-layer DeepGCN model achieves the regression with mean square error (MSE) of 0.011 706 (2.41% mean absolute percentage error) for stress field prediction and 0.002 735 MSE (with 1.58% elements having error larger than 0.01) for topological optimization. The overall concept of the BOGE approach paves the way for a general and efficient deep-learning-based FEA simulator that will benefit both industry and Computer Aided Design (CAD) design-related areas.
Timed concurrent language for argumentation with maximum parallelism
Authors: Bistarelli Stefano , Chiara Meo Maria , Taticchi Carlo 
Published: March 2023

Abstract
The timed concurrent language for argumentation (tcla) is a framework to model concurrent interactions between communicating agents that reason and take decisions through argumentation processes, also taking into account the temporal duration of the performed actions. Time is a crucial factor when dealing with dynamic environments in real-world applications, where agents must act in a coordinated fashion to reach their own goals. However, modelling complex interactions and concurrent processes may be challenging without the help of proper languages and tools. In this paper, we discuss the use of tcla for practical purposes and provide a working implementation of the language, endowed with a user interface available online, that serves the dual purpose of aiding the research in this field and facilitating the development of multi-agent systems based applications.