bluuebunny/crossref_metadata_2025_split huggingface dataset:
Canine Diabetes Mellitus Demonstrates Multiple Markers of Chronic Inflammation Including Th40 Cell Increases and Elevated Systemic-Immune Inflammation Index, Consistent with Autoimmune Dysregulation
Authors: Vaitaitis Gisela , Webb Tracy , Webb Craig , Sharkey Christina , Sharkey Steve , Waid Dan , Wagner David H. 
Published: June 2023

Abstract
Canine diabetes mellitus (CDM) is a relatively common endocrine disease. Many CDM clinical features resemble human type 1 diabetes mellitus (T1DM), but lack of autoimmune biomarkers makes calling the disease autoimmune controversial. Description of autoimmune biomarkers linking CMD and T1DM would create an alternative model for drug development impacting both diseases. We examined peripheral blood of diagnosed CMD dog patients comparing to healthy control (HC) dogs. Differences in adaptive immune lymphocytes, innate immune macrophages/monocytes and neutrophils and differences in platelets were determined. Differences in serum glucose, cholesterol and the liver function enzyme alkaline phosphatase were noted. Markers of disease progression glycated albumin (fructosamine) and c-peptide were determined in CDM. Significant differences in lymphocytes, monocytes, and platelets were found, with increased neutrophils occurring in CDM. A systemic immune inflammation index (SII) and chronic inflammation index (CII) as measures of dynamic changes in adaptive and innate cells between inflammatory and non-inflammatory conditions were created with highly significant differences between CDM and HC. Th40 cells that are demonstrably pathogenic in mouse T1DM and able to differentiate diabetic from non-diabetic subjects in human T1DM were significantly expanded in peripheral blood mononuclear cells. The association of significantly elevated Th40 cells in CDM when compared to HC or to osteoarthritis, a chronic but non-autoimmune disease, demonstrates that CDM involves chronic inflammation. The differences in SII and CII underscore those findings.
Arterial stiffness, high fasting glucose, and fatty liver as predictors for visceral obesity in middle-aged Chinese individuals: a cross-sectional study
Authors: Nawata Hajime , Ou Li , Zhang Xu , Song Qinglan , Huang Jing , Hu Jin , Ito Kazue , Obo Shinichi , Fukushima Takeharu , Iwami Kaori , Iguchi Shizuka , Igarashi Ai , He Xiaoyang , Zhang Jing , Xia Yu , Takasaki Ken 
Published: June 2023

Abstract
Background
The prevalence of obesity is increasing rapidly worldwide, including in Asia. Visceral obesity, i.e., intra-abdominal fat accumulation, precedes the development of obesity-related metabolic syndrome, based on the cluster of hyperglycemia, dyslipidemia, and hypertension, leading to atherosclerosis and cardiovascular disease. Visceral fat area (VFA) ≥ 100 cm2 has been defined as an appropriate threshold for assessing the prevalence of obesity-related metabolic syndrome; however, there information regarding the predictors for VFA ≥ 100 cm2 in middle-aged Chinese individuals in the general population is lacking.
Methods
We investigated the predictors for VFA ≥ 100 cm2 in middle-aged Chinese individuals (n = 148; 49.3 ± 10.8 years; 54% male) who underwent health check-ups. VFA and subcutaneous fat area were determined by computed tomography, and arterial stiffness and fatty liver were diagnosed by brachial-ankle pulse wave velocity (baPWV) and abdominal ultrasonography, respectively. We compared parameters between individuals with VFA ≥ 100 cm2 and < 100 cm2 using unpaired t-tests and Mann-Whitney U tests and identified predictors by binary regression analysis.
Results
Single regression analysis identified body mass index > 25 kg/dL, waist circumference (WC) > 85 cm, red blood cells > 470×104/µL, hemoglobin > 14.2 g/dL, alanine aminotransferase > 29 U/L, uric acid > 5.7 mg/dL, triglycerides > 150 mg/dL, fasting blood glucose (FBG) > 100 mg/dL, baPWV > 1,400 cm/s, male, and fatty liver as significant predictors for VFA ≥ 100 cm2. Multiple regression analysis also identified baPWV > 1,400 cm/s (odds ratio [OR] = 5.82, P = 0.008), WC > 85 cm (OR = 6.37, P = 0.013), FBG > 100 mg/dL (OR = 6.73, P = 0.025), male (OR = 10.54, P = 0.025), and fatty liver (OR = 4.11, P = 0.034) as independent predictors for VFA ≥ 100 cm2. The strongest predictor of VFA ≥ 100 cm2 was baPWV > 1,400 cm/s, with a significant positive correlation (γ = 0.365, P < 0.001).
Conclusions
High baPWV, high FBG, high WC, fatty liver, and male sex are independent predictors for VFA ≥ 100 cm2 in middle-aged Chinese individuals. High baPWV, as a measure of arterial stiffness, was the strongest predictor of VFA ≥ 100 cm2, indicating the importance of visceral obesity for arterial stiffness. Visceral obesity is thus a central target for interventions in metabolic syndrome, metabolic dysfunction-associated fatty liver, and cardiovascular disease, especially in males.
Power suppression in EEG after the onset of SAH is a significant marker of early brain injury in rat models
Authors: Takasugi Yuji , Hishikawa Tomohito , Shimizu Tomohisa , Murai Satoshi , Haruma Jun , Hiramatsu Masafumi , Tokunaga Koji , Takeda Yoshimasa , Sugiu Kenji , Morimatsu Hiroshi , Date Isao 
Published: June 2023

Abstract
We analyzed the correlations among membrane depolarization, changes in electroencephalogram (EEG), and histological outcome in rats in the acute stage of subarachnoid hemorrhage (SAH). SAH was induced in eight rats by cisternal blood injection, and the duration of cortical depolarization was measured. EEG power spectrums were given by time frequency analysis, and histology was evaluated. The appropriate frequency band and recovery percentage of EEG (defined as EEG recovery time) to predict the neuronal damage were determined from 25 patterns (5 bands × 5 recovery rates) of receiver operating characteristic (ROC) curves. Probit regression curves were depicted to evaluate the relationships between neuronal injury and duration of depolarization and EEG recovery. Depolarization and EEG changes on the brain cortex of rats immediately after SAH were successfully recorded. Histological study identified sixteen cites. The optimal values of the EEG band and the EEG recovery time to predict neuronal damage were 10 to 15 Hz and 40%, respectively (area under the curve [AUC]: 0.97). There was a close relationship between the percentage of damaged neurons and the duration of depolarization or EEG recovery time. These results suggest that EEG recovery time may be a predictor of early brain injury (EBI) after SAH.
fast finite-time stability and stabilization of nonlinear impulsive systems
Authors: Cheng Mengqing , Zhao Junsheng , Sun Zong-Yao , Xie Xiangpeng 
Published: June 2023

Abstract
The issue of fast finite-time stability and stabilization design for a class of nonlinear impulsive systems is discussed in this paper. In previous finite-time control designs for impulsive systems, the convergence rate of these control strategies is even less rapid than the exponential convergence rate when the initial state is distant from the origin. To overcome this problem, a nonlinear impulse system is obtained by mathematical modeling of the mechanical buffer. The fast finite-time stability criterion is established for this system, and the upper bound of the settling-time is evaluated. In addition, combining Lyapunov functions theory and impulsive control, we design a controller for impulsive dynamical systems to satisfy fast finite-time stability. Finally, two simulation examples are given,in which the first example demonstrates the superiority of the fast finite-time stability criterion for nonlinear impulsive systems by comparing it with traditional finite-time stability, and the second example verifies the effectiveness of the presented controller.
Genetic parameters for milk yield and reproductive traits in Honduran Holstein cattle
Authors: Estrada-León Raciel Javier , Valladares-Rodas Manuel A. , Sierra-Vázquez Ángel C. , Magaña-Monforte Juan Gabriel , Segura-Correa José Candelario , Parra-Bracamonte Gaspar Manuel 
Published: June 2023

Abstract

The objective of this study was to estimate the (co)variance components and genetic parameters for milk yield (MY), calving-to-conception interval (CCI), number of services per conception (NSC) and calving interval (CI) of Honduran Holstein cows, by fitting a bivariate animal model using Maximum Restricted Likelihood procedures. Model included the fixed effects of, calving number, the contemporary calving group (farm-season-year of calving and the cow age as covariate). The estimated means and standard deviations for MY, CCI, NSC and CI were, 5098.60 ± 1564.32 kg, 168.27 ± 104.71 days, 2.46 ± 1.69 services, and 448.73 ± 109.16 days, respectively; and their estimated heritabilities were 0.21 ± 0.05, 0.03 ± 0.028, 0.02 ± 0.024 and 0.06 ± 0.04, respectively. The genetic correlations between MY305 and CCI, NSC and CI were positive and antagonist, with values of 0.64 ± 0.52, 0.99 ± 0.56, and 0.32 ± 0.24 respectively. Even though moderate to low heritability was estimated for MY, important genetic considering the systematic selection of MY; however, with a reduction in reproductive efficiency, if considered as the only selection criterion. By including reproductive traits and considering permanent environment effects into the breeding program, will yield a slow, but constant and permanent improvement over time.
Association between experience of insulin resistance and long-term cardiovascular disease risk: Findings from the Korean Genome and Epidemiology Study (KOGES)
Authors: Lee Jong Hee , Lee Hye Sun , Jeon Soyoung , Lee Yong-Jae , Park Byoungjin , Lee Jun-Hyuk , KWON YUJIN 
Published: June 2023

Abstract
Purpose
Although the correlation between insulin resistance (IR) and cardiovascular disease (CVD) risk is well established, the impact of changes in IR status over time has received little attention. This study aimed to investigate the effect of IR on CVD risk in a large prospective cohort of middle-aged Korean adults.
Methods
We assessed 3,597 participants from the Korean Genome and Epidemiology Study (KoGES). Participants were categorized as having IR if their HOMA-IR was ≥ 2.5 at least once during the exposure period. Multivariate Cox proportional hazards regression analysis was performed to assess hazard ratios (HRs) with 95% CIs for incident CVD after adjusting for confounders.
Results
Among 3,597 participants, 2,259 did not have IR, and 1,138 had IR. The cumulative incidence rate of CVD in the IR group was significantly higher than that in the non-IR group (log-rank test, p = 0.015). Compared to the non-IR group, the HR and 95% CI for incident CVD in the IR group was 1.40 (1.07–1.83) in the unadjusted model. The presence of IR during the exposure period was significantly associated with a higher risk of incident CVD after adjusting for age, sex, body mass index, diabetes, hypertension, dyslipidemia, high-sensitivity C-reactive protein, physical activity, alcohol intake, and smoking status (HR = 1.37; 95% CI: 1.01–1.84).
Conclusion
Individuals who have experience of IR have a consistently higher likelihood of developing CVD than those who never had IR. More intensive efforts should be made to prevent IR in middle-aged and older adults.
Four-dimensional Hydrogel Dressing Adaptable to the Urethral Microenvironment for Scarless Urethral Reconstruction
Authors: Hua Yujie , Wang Kai , Huo Yingying , Zhuang Yaping , Wang Yuhui , Fang Wenzhuo , Sun Yuyan , Zhou Guangdong , Fu Qiang , Zhang Kaile , Cui Wenguo 
Published: June 2023

Abstract
The harsh urethral microenvironment (UME) after trauma severely hinders the current hydrogel-based urethral repair. In fact, four-dimensional (4D) consideration to mimic time-dependent physiological processes is essential for scarless urethral reconstruction, which requires balancing extracellular matrix (ECM) deposition and remodeling at different healing stages. In this study, we developed a novel UME-adaptable 4D hydrogel dressing to sequentially provide an early-vascularized microenvironment and later-antifibrogenic microenvironment for scarless urethral reconstruction. With the combination of dynamic boronic ester crosslinking and covalent photopolymerization, the resultant gelatin methacryloyl phenylboronic acid/cis-diol-crosslinked (GMPD) hydrogels exhibited mussel-mimetic viscoelasticity, satisfactory adhesion, and acid-reinforced stability, which could adapt to harsh UME. In addition, a temporally on-demand regulatory (TOR) technical platform was introduced into GMPD hydrogels to create a time-dependent 4D microenvironment. As a result, physiological urethral recovery was successfully mimicked by means of an early-vascularized microenvironment to promote wound healing by activating the vascular endothelial growth factor (VEGF) signaling pathway, as well as a later-antifibrogenic microenvironment to prevent hypertrophic scar formation by timing transforming growth factor-β (TGFβ) signaling pathway inhibition. Both in vitro molecular mechanisms of the physiological healing process and in vivo scarless urethral reconstruction in a rabbit model were effectively verified, providing a promising alternative for urethral injury treatment.
Forensic nursing in the emergency department The distance between performed role behaviors by nurses and their perception of behaviors’ importance
Authors: Emami Somayeh Zare , Lynch Virginia A. , Banazadeh Marjan 
Published: June 2023

Abstract
Background
Emergency department nurses often deal with victims of violence and trauma. Lifesaving and stabilizing the patients’ conditions often is a priority and protecting the chain of valuable evidence is the next priority. It is important to describe how nurses currently practice in ED and perceive their role in caring for forensic patients.
Objective
To investigate the performed frequency and perceived importance of forensic nursing role behaviors by ED nurses and the difference between performed behaviors and perceived behaviors’ importance.
Method
A questionnaire was used to investigate the performed frequency and perceived importance of forensic nursing role behaviors among 274 ED nurses.
Results
The total mean score of performed frequency and perceived importance of behaviors was 2.36 ± 0.65 and 4.23 ± 0.64 respectively. The total mean difference score between performed frequency and perceived importance was 1.86. The highest mean difference scores belonged to the role behaviors of forensic nurse specialists including educating the staff on forensic matters (3.1), acting as an expert witness in court (3.09), and operating in the ED (3.08). The items with the lowest mean difference scores were: informing forensic physicians about admitting a dead patient on arrival (0.93), assisting police investigators in their mission (0.99), and assuring documentation for investigation/legal protection of the nurse/patient (1.01). Performed frequency of behaviors positively correlated with participants’ type of shift worked and their personal experiences of legal and judicial claims. The female gender of respondents positively correlated with behaviors’ perceived importance.
Conclusion
The gap between role behaviors enacted by ED nurses and their expectations to perform those behaviors reflects an urgent need to train nurses by integrating forensic nursing topics into graduate and undergraduate nursing curricula, nursing programs, and in-service education. Forensic nurses should be available in ED to facilitate the conviction of criminals and support the victims of trauma and innocents. To meet society’s changing needs and demands ED nurses should be encouraged to expand their. Interventions should be undertaken to specialize nursing roles and recognize forensic nursing specialty. It is required to develop and apply forensic nursing protocols in caring for victims of trauma and violence and facilitate the cooperation of healthcare system with law enforcement and forensic investigators.
Effect of Drought Stress on the Physiology and Early Growth of Seven Senegalia senegal (L.) Britton Provenances
Authors: Sarr Mame Sokhna , Seiler John R. , Sullivan Jay 
Published: June 2023

Abstract
Decline of Senegalia senegal in its natural range has been observed and attributed mainly to harsh environmental conditions such as frequent drought occurrences. The objective of this study is to assess the effect of water stress on growth, biomass allocation and photosynthetic capacity in Senegalia senegal seedlings. Seeds were collected from seven provenances in Senegal and grown under greenhouse conditions in a randomized complete block design with 5 replications. Seedlings were subjected to three cyclic droughts by watering them when average soil moisture content dropped to 4.7%, 2.7%, and 2.1% during the first, second, and third dry-down cycles, respectively. With the exception of net photosynthetic rate, stomatal conductance and transpiration at the second and third dry down cycles, no drought by provenance interaction was found for any trait measured. Ngane provenance had a lower root/shoot ratio and allometric analysis revealed that Ngane seemed to allocate less biomass to roots than shoots. Ngane developed superior growth traits and biomass production, despite similar net photosynthetic rate with other provenances before treatments started. Overall, drought stress significantly reduced biomass, stem height, diameter and leaf gas exchange. Total biomass was reduced by 28.5%, whereas root/shoot ratio was increased by 25% compared to control. Significant interaction on leaf gas exchanges at the second and third dry down cycles reveals different sensitivity levels to drought among provenances with the Ranerou provenance exhibiting no decline in leaf gas exchange. No significant difference of stomatal limitation among genotypes was found, which is indicative of the complex process of photosynthesis and the need for extended times scales for measurements to better depict physiological sensitivity of Senegalia senegal under drought.
Andrographolide Synergized with Doxorubicin to Induce Cell Death in Breast Cancer Cell via ROS-dependent DNA Damage
Authors: Xie Changquan , Lu Guo-Dong , Li Qiu-yun 
Published: June 2023

Abstract
Breast cancer is a malignant tumor that seriously endangers women’s health all over the world. Doxorubicin is a commonly used chemotherapeutic drug for the treatment of breast cancer. However, its application is hindered by toxic side effects and drug resistance. A combination strategy must be found to increase the sensitivity of human breast cancer cells to doxorubicin and enhance its anticancer efficacy. Andrographolide (Andro) is a good candidate drug because of its good tolerance and anticancer efficacy. In this study, we found that andrographolide enhanced cell death caused by doxorubicin both in MDA-MB-231 and MCF-7 breast cancer cell lines. Furthermore, the combination treatment greatly increased the anti-cancer effect to MDA-MB-231 cell xenograft compared with every single treatment in Balb/c nude mouse model. Mechanistically, compared with andrographolide or doxorubicin alone treatment, the combined treatment significantly increased DNA damage by inducing ROS production, leading to cell death. In conclusion, andrographolide can be used to improve the sensitivity of doxorubicin in the treatment of breast cancer, providing a basis for the transformation of combined treatment into clinical application.
Active sound source localization in bilateral hearing-impaired children
Authors: Gulli Andrea , Fontana Federico , Orzan Eva , Aruffo Alessandro , Muzzi Enrico 
Published: June 2023

Abstract
This study investigates auditory localization in children with no motor deficits and a diagnosis of hearing impairment rehabilitated with bilateral cochlear implants or hearing aids. The contribution of head movement to sound source localization in the anterior horizontal field has been analyzed. A localization task took place in a virtual environment, where participants who could move their heads freely were asked to point to an invisible sound source. The source was rendered using a loudspeaker set arranged in a semi-circular array in the horizontal plane, while participants observed a neutral visual scene through a head-mounted display. This device also allowed for tracking the participants’ head and hand positions while they pointed to the auditory target. Investigating conditions in which no, one, or both ear devices were turned off, we found that asymmetrical hearing caused the largest head movements. The correlation between localization accuracy and pure tone audiometric thresholds measured when the ear devices were turned off as well as the correlation between such thresholds and the amount of head movement support the hypothesis that horizontal auditory localization is an ability that develops in synergy with active listening skills. This finding may have important implications for the clinical assessment and rehabilitation of adults and children affected by hearing impairment.
Mechanisms of Xiaoyao Huaxian Formula in the treatment of liver fibrosis based on network pharmacology combined with molecular docking and in vivo experiments
Authors: Wang Qiong , Li Ting , He Yansha , Liu Huabao , Hu Changlun , Rao Chunyan 
Published: June 2023

Abstract
Purpose Xiaoyao Huaxian Formula (XYHXF) shows promise in treating liver fibrosis (LF), but the mechanism is unknown. We sought to elucidate this using network pharmacology, docking and animal experiments.Methods The overlapping targets were determined between those of the main active ingredients of XYHXF using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and the Traditional Chinese Medicine Integrated Database and LF-related targets from the Therapeutic Target Database, Online Mendelian Inheritance in Man, and DisGeNET. Protein-protein interaction (PPI) network was generated with Cytoscape 3.7.2. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using R language. Docking was conducted with AutoDockTools for the key targets and active ingredients of XYHXF. An LF rat model was established with subcutaneously injected carbon tetrachloride followed by ethanol gavage and feeding a high-fat, low-protein diet, and an oral dose of 2.72 g/kg of XYHXF was tested in this model. Liver function was assessed with serum ALT and AST measurements, HE and Masson staining, and western blot of TGF-β, α-SMA, PI3K, p-PI3K, AKT, and p-AKT proteins in liver.Results Overall, 143 active ingredients of XYHXF with 520 potential targets and 2,070 targets of LF were retrieved. The 220 overlapping targets included STAT3, MAPK3, MAPK1, AKT1, and SRC. GO and KEGG analysis revealed overlapping targets that included the response to lipopolysaccharide, response to molecule of bacterial origin, reactive oxygen species metabolic process, and PI3K-Akt signaling pathway. Docking demonstrated potential binding between quercetin/luteolin and AKT1. In rats, compared to the LF group, the XYHXF group had significantly attenuated liver injury and fibrosis, reductions in ALT, AST, α-SMA, and TGF-β; and down-regulation of p-PI3K/PI3K and p-AKT/AKT ratios.Conclusion XYHXF may act on inflammatory processes via multiple active ingredients and treat LF by regulating PI3K-Akt signaling.