bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen VI expression is negatively mechanosensitive in pancreatic cancer cells and supports the metastatic niche
Authors: Papalazarou Vasileios , Drew James , Juin Amelie , Spence Heather J. , Whitelaw Jamie , Nixon Colin , Salmeron-Sanchez Manuel , Machesky Laura M. 
Published: December 2022

ABSTRACT
Pancreatic cancer is a deadly and highly metastatic disease, although how metastatic lesions establish is not fully understood. A key feature of pancreatic tumours is extensive fibrosis and deposition of extracellular matrix (ECM). While pancreatic cancer cells are programmed by stimuli derived from a stiff ECM, metastasis requires loss of attachment and adaptation to a softer microenvironment at distant sites. Growing evidence suggests that stiff ECM influences pancreatic cancer cell behaviour. Here, we argue that this influence is reversible and that pancreatic cancer cells can be reprogrammed upon sensing soft substrates. Using engineered polyacrylamide hydrogels with tuneable mechanical properties, we show that collagen VI is specifically upregulated in pancreatic cancer cells on soft substrates, due to a lack of integrin engagement. Furthermore, the expression of collagen VI is inversely correlated with mechanosensing and activity of YAP (also known as YAP1), which might be due to a direct or indirect effect on transcription of genes encoding collagen VI. Collagen VI supports migration in vitro and metastasis formation in vivo. Metastatic nodules formed by pancreatic cancer cells lacking Col6a1 display stromal cell-derived collagen VI deposition, suggesting that collagen VI derived from either cancer cells or the stroma is an essential component of the metastatic niche.
This article has an associated First Person interview with Vasileios Papalazarou, joint first author of the paper.
Endosomal vesicle fusion machinery is involved with the contractile vacuole in Dictyostelium discoideum
Authors: Manna Paul T. , Barlow Lael D. , Ramirez-Macias Inmaculada , Herman Emily K. , Dacks Joel B. 
Published: December 2022

ABSTRACT
Contractile vacuoles (CVs), enigmatic osmoregulatory organelles, share common characteristics, such as a requirement for RAB11 and high levels of V-ATPase. These commonalities suggest a conserved evolutionary origin for the CVs with implications for understanding of the last common ancestor of eukaryotes and eukaryotic diversification more broadly. A taxonomically broader sampling of CV-associated machinery is required to address this question further. We used a transcriptomics-based approach to identify CV-associated gene products in Dictyostelium discoideum. This approach was first validated by assessing a set of known CV-associated gene products, which were significantly upregulated following hypo-osmotic exposure. Moreover, endosomal and vacuolar gene products were enriched in the upregulated gene set. An upregulated SNARE protein (NPSNB) was predominantly plasma membrane localised and enriched in the vicinity of CVs, supporting the association with this organelle found in the transcriptomic analysis. We therefore confirm that transcriptomic approaches can identify known and novel players in CV function, in our case emphasizing the role of endosomal vesicle fusion machinery in the D. discoideum CV and facilitating future work to address questions regarding the deep evolution of eukaryotic organelles.
Reorganization of the DNA replication landscape during adipogenesis is closely linked with adipogenic gene expression
Authors: Hayakawa Takuya , Yamamoto Asahi , Yoneda Taiki , Hori Sakino , Okochi Nanami , Kagotani Kazuhiro , Okumura Katsuzumi , Takebayashi Shin-ichiro 
Published: December 2022

ABSTRACT
The temporal order of DNA replication along the chromosomes is thought to reflect the transcriptional competence of the genome. During differentiation of mouse 3T3-L1 cells into adipocytes, cells undergo one or two rounds of cell division called mitotic clonal expansion (MCE). MCE is an essential step for adipogenesis; however, little is known about the regulation of DNA replication during this period. Here, we performed genome-wide mapping of replication timing (RT) in mouse 3T3-L1 cells before and during MCE, and identified a number of chromosomal regions shifting toward either earlier or later replication through two rounds of replication. These RT changes were confirmed in individual cells by single-cell DNA-replication sequencing. Coordinate changes between a shift toward earlier replication and transcriptional activation of adipogenesis-associated genes were observed. RT changes occurred before the full expression of these genes, indicating that RT reorganization might contribute to the mature adipocyte phenotype. To support this, cells undergoing two rounds of DNA replication during MCE had a higher potential to differentiate into lipid droplet-accumulating adipocytes, compared with cells undergoing a single round of DNA replication and non-replicating cells.
The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host–microbe interactions under warming
Authors: Fontaine Samantha S. , Kohl Kevin D. 
Published: December 2022

ABSTRACTPhenotypic plasticity is an important strategy that animals employ to respond and adjust to changes in their environment. Plasticity may occur via changes in host gene expression or through functional changes in their microbiomes, which contribute substantially to host physiology. Specifically, the presence and function of host-associated microbes can impact how animals respond to heat stress. We previously demonstrated that ‘depleted’ tadpoles, with artificially disrupted microbiomes, are less tolerant to heat than ‘colonized’ tadpoles, with more natural microbiomes. However, the mechanisms behind these effects are unclear. Here, we compared gene expression profiles of the tadpole gut transcriptome, and tadpole gut microbial metagenome, between colonized and depleted tadpoles under cool or warm conditions. Our goal was to identify differences in host and microbial responses to heat between colonized and depleted tadpoles that might explain their observed differences in heat tolerance. We found that depleted tadpoles exhibited a much stronger degree of host gene expression plasticity in response to heat, while the microbiome of colonized tadpoles was significantly more heat sensitive. These patterns indicate that functional changes in the microbiome in response to heat may allow for a dampened host response, ultimately buffering hosts from the deleterious effects of heat stress. We also identified several specific host and microbial pathways that could be contributing to increased thermal tolerance in colonized tadpoles including amino acid metabolism, vitamin biosynthesis and ROS scavenging pathways. Our results demonstrate that the microbiome influences host plasticity and the response of hosts to environmental stressors.
Trends of research topics related to Halal meat as a commodity between Scopus and Web of Science: A systematic review
Authors: Nafik Hadi Ryandono Muhammad , Mawardi Imron , Nugraha Rani Lina , Widiastuti Tika , Tri Ratnasari Ririn , Kusuma Wardhana Akhmad 
Published: December 2022

Background: People are more aware of halal products in commodity markets. Halal meat is an essential commodity for society in the Islamic commodity market in particular. The objective of this study was to analyze differences in research trends related to halal meat in published papers indexed by Scopus and Web of Science. The objective is to observe how was halal meat as commodity become the trend of research written by Indonesian authors, as well as what was the novelty of the research trend related to such tiopic. Methods: This study used purposive sampling with a bibliometric approach in collecting the samples. The samples were published papers from the Scopus and Web of Science (SCI) databases from 2006 to 25th October 2021. The were 154 samples found from cSopus and 50 samples in Web of Science (SCI). Analysis was performed by VOSviewer and biblioshiny. Results: Overall, 154 papers from Scopus and 50 from SCI were analyzed. Scopus and SCI exhibited different research trends. Moreover, papers from Scopus were dominated by Indonesian authors; those from SCI were not. The source growth of published papers in SCI was more dynamic than that in Scopus. The research trends in papers from SCI were more focused on the types of meat and the source itself. This was different to papers in Scopus, which placed greater emphasis on the supply chain. Conclusions: This paper can act as a reference for continued research into halal meat in commodity markets, especially in Islamic countries. This paper is the first to compare the research trends in halal meat in the commodity market by comparing the differences in two major indexes, Scopus and SCI.
The relationship between climate change and malaria in South-East Asia: A systematic review of the evidence
Authors: Rahmani Ardhi Arsala , Susanna Dewi , Febrian Tommi 
Published: December 2022

Background: Climatic change is an inescapable fact that implies alterations in seasons where weather occurrences have their schedules shift from the regular and magnitudes intensify to more extreme variations over a multi-year period. Southeast Asia is one of the many regions experiencing changes in climate and concurrently still has endemicities of malaria. Given that previous studies have suggested the influence of climate on malaria’s vector the Anopheles mosquitoes and parasite the Plasmodium group, this study was conducted to review the evidence of associations made between malaria cases and climatic variables in Southeast Asia throughout a multi-year period. Methods: Our systematic literature review was informed by the PRISMA guidelines and registered in PROSPERO: CRD42022301826 on 5th February 2022. We searched for original articles in English and Indonesian that focused on the associations between climatic variables and malaria cases. Results: The initial identification stage resulted in 535 records of possible relevance and after abstract screening and eligibility assessment we included 19 research articles for the systematic review. Based on the reviewed articles, changing temperatures, precipitation, humidity and windspeed were considered for statistical association across a multi-year period and are correlated with malaria cases in various regions throughout Southeast Asia. Conclusions: According to the review of evidence, climatic variables that exhibited a statistically significant correlation with malaria cases include temperatures, precipitation, and humidity. The strength of each climatic variable varies across studies. Our systematic review of the limited evidence indicates that further research for the Southeast Asia region remains to be explored.
Until the dawn: everyday experiences of people living with COVID-19 during the pandemic in Thailand
Authors: Imkome Ek-uma , Moonchai Kamonchanok 
Published: December 2022

Background: This study aims to understand and analyze the complex experiences of people living with COVID-19 to support future cases. Methods: Purposive and snowball sampling techniques were employed to collect data through in-depth interviews; the data were combined with non-aggregated behavioral observations. Lived experiences of 15 individuals were collected through semi-structured interviews and analyzed using a phenomenological-hermeneutic approach. We used the thematic analysis technique to analyze the data. Results: Through analysis, themes of stress, economic impact, social stigma, social support, and unexpected benefits were identified. Participants further expressed economic and social concerns during the interviews. Conclusion: The identified themes can help develop multidisciplinary treatment strategies that would be useful during a healthcare crisis, establish comprehensive support systems that could address economic and social problems, and provide training for employees for post-COVID-19 assistance.
Modified recurrent equation-based cubic spline interpolation for missing data recovery in phasor measurement unit (PMU)
Authors: Thangaraj Shruthi , Goh Vik Tor , Yap Timothy Tzen Vun 
Published: December 2022

Background: Smart grid systems require high-quality phasor measurement unit (PMU) data for proper operation, control, and decision-making. Missing PMU data may lead to improper actions or even blackouts. While the conventional cubic interpolation methods based on the solution of a set of linear equations to solve for the cubic spline coefficients have been applied by many researchers for the interpolation of missing data, the computational complexity increases non-linearly with increasing data size. Methods: In this work, a modified recurrent equation-based cubic spline interpolation procedure for recovering missing PMU data is proposed. The recurrent equation-based method makes the computations of spline constants simpler. Using PMU data from the State Load Despatch Center (SLDC) in Madhya Pradesh, India, a comparison of the root mean square error (RMSE) values and time of calculation (ToC) is calculated for both methods. Results: The modified recurrent relation method could retrieve missing values 10 times faster when compared to the conventional cubic interpolation method based on the solution of a set of linear equations. The RMSE values have shown the proposed method is effective even for special cases of missing values (edges, continuous missing values). Conclusions: The proposed method can retrieve any number of missing values at any location using observed data with a minimal number of calculations.
DIÁLOGO FILOSÓFICO COM O TEXTO “UMA FENOMENOLOGIA DO HOMEM CAPAZ”, DE PAUL RICOEUR
Authors: Rueda Neto Eduardo 
Published: December 2022

Este artigo propõe um breve diálogo filosófico com o texto “Uma fenomenologia do homem capaz”, do livro Percurso do reconhecimento, de Paul Ricoeur. Nesse texto, o filósofo desenvolve uma antropologia fenomenológica a partir da análise de quatro capacidades inerentes e constitutivas da identidade humana: o dizer, o fazer, o narrar e o imputar. No trajeto seguido pelo autor, abrem-se inúmeras janelas para diferentes discussões que se ramificam em outras tantas possibilidades de reflexão e dialética. O objetivo deste ensaio é explorar de forma embrionária essas potencialidades do texto ricoeriano, com o fim de viabilizar futuras discussões mais aprofundadas com base nas sendas indicadas. Por fim, o artigo conclui endossando a ideia de que é no exercício de suas capacidades intrínsecas que o ser humano constrói sua própria identidade, num processo autorreflexivo que se aprofunda e amplia na medida em que interage consigo, com o meio e com o outro.

Palavras-chave: ser humano; capacidade; reflexividade; identidade.
Welcoming and educational care in multicultural society
Authors: Indellicato Rosa 
Published: December 2022

In a highly multicultural society, it is right to speak of welcome and educational care, a perfect combination that should characterise all educational contexts starting with the family and the school. Welcome and care have a strong impact on the educational and training context. A foreign child who finds himself in a new environment should feel welcomed, recognised, valued in his original and individual way of being, the bearer of his own history, and this should happen during his growth and learning process. Taking care of the child, having a genuine interest in him, in his well-being and growth, requires special attention to his education and training.
FMR1 CGG Repeats and Stress Influence Self-Reported Cognitive Functioning in Mothers
Authors: Maltman Nell , DaWalt Leann Smith , Hong Jinkuk , Baker Mei Wang , Berry-Kravis Elizabeth M. , Brilliant Murray H. , Mailick Marsha 
Published: December 2022

Abstract
Variation in the FMR1 gene may affect aspects of cognition, such as executive function and memory. Environmental factors, such as stress, may also negatively impact cognitive functioning. Participants included 1,053 mothers of children with and without developmental disabilities. Participants completed self-report measures of executive function, memory, and stress (i.e., life events, parenting status), and provided DNA to determine CGG repeat length (ranging from 7 to 192 CGGs). Stress exposure significantly predicted greater self-reported difficulties in executive function and the likelihood of memory problems. Cubic CGG effects independently predicted executive function and memory difficulties, suggesting effects of both genetic variation and environmental stress exposure on cognitive functioning.
Intimate partner violence during pregnancy and adverse birth outcomes in Ethiopia: A systematic review and meta-analysis
Authors: Belay Habtamu Gebrehana , Debebe Getachew Arage , Ayele Alemu Degu , Kassa Bekalu Getnet , Mihretie Gedefaye Nibret , Bezabih Lealem Meseret , Worke Mulugeta Dile 
Published: December 2022

Background
Intimate partner violence is a significant public health issue that affects maternal and neonatal health worldwide. Several studies have been conducted to investigate the prevalence of intimate partner violence during pregnancy as well as the factors that contribute to it. As a result, the purpose of this study was to determine the impact of intimate partner violence on birth outcomes.

Methods
International databases including Scopus, PubMed, Google Scholar, Embase, and CINAHL were used to search primary studies. The quality and strength of the included studies were evaluated using the Newcastle-Ottawa Scale quality assessment tool. The studies heterogeneity and publication biases were assessed using I2 statistics and Egger’s regression test. The Meta-analysis was carried out using STATA version 16 software.

Results
A total of nine hundred and fifty-eight articles were retrieved from various databases, and seventeen articles were included in the review. The pooled prevalence of intimate violence during pregnancy in Ethiopia was 32.23% (95% CI 28.02% -36.45%). During pregnancy, intimate partner violence was a significant predictor of low birth weight (AOR: 3.69, 95%CI 1.61–8.50) and preterm birth (AOR: 2.23, 95%CI 1.64–3.04).

Conclusion
One in every three pregnant women experiences intimate partner violence. Women who experienced intimate partner violence during their pregnancy are more likely to experience adverse outcomes such as premature delivery and low birth weight infants.
Higher intraoperative mean arterial blood pressure does not reduce postoperative delirium in elderly patients following gastrointestinal surgery: A prospective randomized controlled trial
Authors: Zhang Yanke , Zhang Ying , Zhou Zhou , Sang Xiaoqiao , Qin Miaomiao , Dai Guangrong , Zhao Zhibin , Yan Fang , Zhang Xiaobao 
Published: December 2022

BackgroundThis study aimed to describe the relationship between the different levels of intraoperative mean arterial blood pressure (MAP) and postoperative delirium in elderly patients undergoing gastrointestinal laparoscopic surgery.Materials and methodsThis prospective controlled clinical trial enrolled 116 patients aged 65 to 85 years who underwent gastrointestinal laparoscopic surgery. These patients were randomized 1:1 to a MAP goal of 65 to 85 mmHg (L group) or an 86 to 100 mmHg (H group). The primary endpoint was the incidence of postoperative delirium, assessed twice daily with the Confusion Assessment Method (CAM) and Richmond Agitation–Sedation Scale (RASS) during the first five postoperative days. Delirium severity was evaluated with the Delirium-O-Meter (D-O-M).Results108 patients (L group n = 55, H group n = 53) were eventually included in intention-to-treat analyses. Postoperative delirium occurred in 18 (32.7%) of 55 cases of L group and in 15 (28.3%) of 53 cases of H group. The incidence of delirium subtypes between the two groups: hypoactive delirium 14.5% (8/55) vs 11.3% (6/53); hyperactive delirium 7.3% (4/55) vs 3.8% (2/53); mixed delirium 10.9% (6/55) vs 13.2% (7/53). However, the L group showed higher D-O-M scores of the first episode of delirium: 14.5 (Q1 = 12, Q3 = 18.5) vs 12 (Q1 = 10, Q3 = 14), which means the delirium is more severe.ConclusionsCompared with 65 to 85 mmHg, maintaining intraoperative MAP at 86–100 mmHg did not reduce the incidence of postoperative delirium in elderly patients undergoing gastrointestinal laparoscopic surgery. However, the severity of delirium could be reduced and blood loss is a risk factor for postoperative delirium.
Assessment of snakebite management practices at Meserani Juu in Monduli District, Northern Tanzania
Authors: Iddi Shabani , Justin Joseph , Hamasaki Kayo , Konje Eveline T. , Kongola Gilbert W. 
Published: December 2022

Background
Snakebite envenoming represents a tragically neglected tropical disease mostly affecting poor people living in remote areas of developing countries, primarily in sub-Saharan Africa. Anti-snake venom (ASV) is the only approved specific treatment for systemic envenoming from snakebite, but it remains largely unavailable in many parts of developing countries. There is paucity of data on snakebite management practice in Tanzania. This study aimed at assessing the community management practices of snakebite and availability of anti-snake venom in the public health facilities in Monduli District, Northern Tanzania.

Methods
A cross sectional study was carried out between May and June, 2018 involving 67 victims, 147 other household members, and 35 public health facilities. A structured questionnaire, respondent interview, and health facility report/document review were considered during data collection. Clean data were analyzed using SPSS version 20.

Results
Sixty-seven snakebite victims and 147 other household members were interviewed during a household survey. All snakebite cases reported to having visited a health facility after snakebite with the majority 55/67 (82.1%) reporting the use, prior attendance to medical care, of some form of local treatment such as tourniquets 13 (19.4%), local incision 11 (16.4%), and snakestone 7 (10.4%). None of the public health facilities in Monduli District attended a snakebite case and had never stocked anti-snake venom products. In this area, 45 snakebite cases were reported to be managed at Meserani snake park clinic where anti-snake venom products were available and provided for free in the period between January 2017 and December 2017.

Conclusion
Majority of the snakebite cases at Meserani Juu relied on local methods for the management of snake bites of which most are of unknown efficacy and safety. Furthermore, none of the primary public health facilities in Monduli District stocked antivenom despite being a habitat for different kinds of venomous snakes. The government and local non-government organizations should collaborate so as to improve the anti-snake venom availability and the provision of snakebite preventive and management awareness programs, especially to the rural communities.