bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium Scoring with Prospective ECG-Triggered coronary CT Angiography: A Path to Reduce Radiation Dose
Authors: Hagar Muhammad Taha , Soschynski Martin , Benndorf Matthias , Stein Thomas , Taron Jana , Schlett Christopher L. , Bamberg Fabian , Krauß Tobias 
Published: March 2023

Abstract
Objectives:
To examine if calcium scoring CT (CAS-CT) reduces the whole-examination radiation dose of prospectively ECG-triggered coronary CT-angiography (CCTA).
Methods
In this retrospective study, patients underwent CAS-CT and prospectively ECG-triggered CCTA on a 2nd generation Dual-Source CT scanner. CCTA was planned on CAS-CT images. We further simulated CCTA-planning on scout-view. Therefore, the scan length of the scout-view-derived CCTA was set equal to the CAS-CT scan length. Effective doses were compared for the following scenarios: (1) CAS-CT-derived CCTA + CAS-CT and (2) scout-view-derived CCTA without CAS-CT. Dose differences between the scenarios were additionally examined with respect to scan mode and body-mass-index.
Results
Among 182 patients (58±12 years, 47% females), planning cCTA on CAS-CT resulted in a shorter scan length than planning on scout-view (114.3 ± 9.7 mm vs 133.7 ± 13.2 mm, p<0.001). The whole-examination effective dose was slightly lower for scenario (1) (3.2 [1.8 – 5.3] mSv vs 3.4 [1.5 – 5.9] mSv; p<0.001, n=182). Scenario (1) resulted in a substantially lower radiation dose in sequential scans (3.6 [2.3 – 6.1] mSv vs 3.9 [2.4 – 6.50] mSv, n=150), or in obese patients (6.8 mSv [4.5 – 9.1]) vs 7.3 mSv [4.7 – 9.9], n=45), p<0.001 respectively. Only in high-pitch spiral CCTA, scenario (2) resulted in a dose salvage (0.8 mSv [0.6 – 1.4] vs 1.0 mSv [0.8 – 1.5], n=32; p<0.001).
Conclusions
Planning prospectively ECG-triggered CCTA on CAS-CT reduces the overall radiation dose of the examination compared to a scout-view planning approach where no CAS-CT is acquired. Only for high-pitch spiral CCTA a slightly opposite effect was observed.
AFDD-LSTSNET-SARIMA Based Short-Term Net Load Forecasting for Customers
Authors: Wang Jingdong , Zhou Jianchen , Cheng Zhu , Qu Guangqiang 
Published: March 2023

Abstract
In order to improve the accuracy of short-term load forecasting for users, an adaptive frequency-domain decomposition (AFDD), long-short term skip neural network (LSTSNET) and the AFDD-LSTSNET-SARIMA short-term load forecasting model is proposed by combining the seasonal autoregressive integrated moving average model (SARIMA). Firstly, AFDD is used to find the optimal combination of all potential periodicity in the frequency domain by using genetic algorithm, thus decomposing the load into periodic and non-periodic components. Based on this, SARIMA and LSTSNET are used to predict the periodic and acyclic components respectively; finally, the prediction results of each component are recombined to obtain the final prediction results of short-term net load of users. The AFDD-LSTSET-SARIMA model is validated on the measured data set of a community in New York State, U.S.A. The experimental results show that the proposed model overcomes the problem of high randomness and fluctuation of net load and effectively improves the accuracy of short-term net load prediction of customers.
A novel de novo splicing mutation of STXBP1 in epileptic encephalopathy associated with hypomyelinating leukodystrophy
Authors: Wang Zixuan , Zhang Jun , Zhou Yunfei , Wang Juan , Liu Guicen , Tian Zixin 
Published: March 2023

Abstract
Background: Deleterious variations in STXBP1 are responsible for early infantile epileptic encephalopathy type 4 (EIEE4, OMIM # 612164) because of its dysfunction in the central nervous system. The clinical spectrum of the neurodevelopmental delays associated with STXBP1 aberrations is collectively defined as STXBP1 encephalopathy (STXBP1-E), the conspicuous features of which are highlighted by early-onset epileptic seizures without structural brain anomalies. The girl first diagnosed with the unexplained disorders of movement and cognitive later was developed into STXBP1-E with unexpected leukoaraiosis and late-onset of seizures.
Methods: The genetic screening and molecular tricks including trio Whole Exome Sequencing (WES), Sanger sequencing, bioengineering with CRISPR/Cas9, Western blot, qPCR/RT-PCR and bioinformatic analyses alongside neurological examinations were employed to investigate the genetic etiology and established the diagnosis.
Results: A novel de novo heterozygous mutation of c.37+2dupT at STXBP1 splice site was identified as the pathogenic cause in the affected girl. The de novo mutation (DNM) didn't result in any truncated proteins, but immediately trigger mRNA degradation by nonsense-mediated mRNA decay (NMD), which led to the haploinsufficiency of STXBP1. The patient showed the atypical phenotypes characterized by hypomyelinating leukodystrophy and late-onset of epileptic seizures, which had never previously been delineated in STXBP1-E.
Conclusion: The findings strongly implicated that the haploinsufficiency of STXBP1 could also exhibit divergent clinical phenotypes because of the genetic heterogeneity in the subset of encephalopathies.
Comparison of the Wound Healing Activity of the Leaf and Leaf Ash Extracts of Vernonia amygdalina in Rats
Authors: Matovu Isaac Joram , Bogere Cissy , Othieno James , Nakabiri Winfred , Kahwa Ivan , Ajayi Clement Olusoji , Omara Timothy , Iqbal Shabnoor 
Published: March 2023

Abstract
Background: Species in the genus Vernonia (Asteraceae) are traditionally used in the treatment of
different ailments, for example, leaves in both fresh and dry forms have been used in dressing wounds.
Anecdotal reports show that the ash of Vernonia amygdalina (V. amygdalina) has been used in the
treatment of wounds, but there are no precise reports to support this claim; therefore, the
contemporary study focused on comparing the wound healing activity of its leaf extracts and leaf ash.
Methods: The study investigating the wound healing activity of V. amygdalina leaf was carried out in
Wistar Albino rats in an excision wound model using its aqueous extract (AE) and leaf ash (LA) at
concentrations of 1%, 2% and 3% (w/v). Mupirocin ointment and distilled water were used as positive
and negative controls, respectively. The wound diameter was measured every 3 days from day 1 to
day 22 and the results were used to calculate the percentage of wound reduction in all groups.
Results: Significant wound healing activity was observed in all groups except 3% AE (w / v), against
distilled water (negative control group) (p<0.05). Group 2 (1% AE), group 5 (1% LA), and group 6
(2% LA) showed faster wound healing than the positive control (p<0.05). Leaf ash was shown to have
the best wound healing activity, and its lower concentrations worked better than the category of the
same concentrations in aqueous extracts.
Conclusions: Our findings have for the first time confirmed the traditional claim of using V. amygdalina
ash in the treatment of topical wounds.
The Impact of Environmental Factors on Traits of Hedera Helix L. Vegetative Shoots
Authors: Blinkova Olena , Rawlik Katarzyna , Jagodziński Andrzej M. 
Published: March 2023

Abstract
Hedera helix L. is an evergreen vine native to Europe. Nowadays it is commonly recognised as an expansive species, posing danger to biodiversity of natural ecosystems. Obtaining the relationships between morphological variability, productive parameters of vegetative individuals and variations in environmental factors (on the example of the Kórnik Arboretum) is important for understanding the successful adaptation and dispersion of H. helix. Thus, the issue of our studies was to find the link between environmental factors and different traits of H. helix. We collected data on leaf length, leaf width, leaf petiole length, plant stem length, and the number of leaves per stem. We assessed values of ecological plasticity and correlation between the studied parameters. The results indicated the tolerance of ivy to wide variation in edaphic conditions. The limiting environmental factors for growth of the studied subpopulations were soil moisture and soil shade. We found large variability of leaf morphometric parameters and shoot length. Close correlation links were detected between leaf length/leaf width, leaf length/leaf petiole length, and leaf width/leaf petiole length. The length of the average leaf was a more variable parameter than the width of the leaf under different values of light intensity. Weak relationships were found between light intensity at the soil surface and plant stem length/the number of leaves per shoot. A high level of index of morphological integration of H. helix individuals and a close relationship between the quality index/subpopulation composition were established in this study. A direct relationship between light intensity and specific leaf area values was established. The value of specific leaf area decreased linearly with increasing of light intensity at the soil surface.
Moss bank composition on the Galindez Island (Argentine Islands): what it signifies?
Authors: WIERZGOŃ Mariusz , IVANETS Viktoria , PREKRASNA-KVIATKOVSKA Yevheniia , PLÁŠEK Vítězslav , PARNIKOZA Ivan 
Published: March 2023

Abstract
Tall moss turf subformation, whose developed forms are called moss banks (MB), play an important role in the structure of vegetation communities in the maritime Antarctic. In the present research, we studied the spatial distribution, moss banks thickness, area, species amount, dependence between these parameters, and species composition of the 44 MB on Galindez Island, Argentine Islands, Graham Coast. To select indicators for the monitoring of communities affected by climate change and biotic disturbance we compared the key parameters of the largest moss bank on Galindez Island (Smith moss bank) with measurements received for this moss bank 46 years ago. Galindez moss banks’ bryophyte flora included 13 species of mosses and three species of liverworts. There was no correlation between the species richness and the area of MB, but the species richness positively correlated with moss banks’ thickness. We supposed species diversity of MB depended presumably on the age of the moss bank and micro-conditions than on the area. Abundance of some moss species correlated with moss bank thickness. A comparison of results obtained in this study and in 1976, revealed an increase in the amount of brown- and black-coloured curtains of Polytrichum strictum, no significant changes in the ratio of Chorisodontium aciphyllum and a decrease in the lichen incrustation. These results show that the ratio of P. strictum’s colour morphotypes, C. aciphyllum and liverworts’ abundance can be used to evaluate the condition of MB in the long-term monitoring.
Assessment Method of Cloud Service Trusted State Based on Fuzzy Entropy and Markov chain
Authors: Yang Ming , Jia Li , Wang Jia , Jiang Rong , Gao Tilei 
Published: March 2023

Abstract
In the era of cloud service popularization, the trustworthiness of service is particularly important. If users cannot prevent the potential trustworthiness problem of the service during long-term use, once the trustworthiness problem occurs, it will cause significant losses. In order to objectively assess the cloud service trustworthiness, and predict its change, this paper establishes a special hierarchical model of cloud service trustworthiness attributes. This paper proposes corresponding management countermeasures around the model, defines the cloud service trustworthiness level, defines the cloud service trusted state based on fuzzy entropy and Markov chain, constructs the membership function of the cloud service trusted state, and realizes the assessment of cloud service trustworthiness and its change according to the prediction method of Markov chain. Through case analysis and method comparison, it shows that the method proposed in this paper is effective and feasible. This method can provide objective and comprehensive assessment data for the cloud service trustworthiness and its change, makes up the deficiency of fuzzy entropy assessment method. This research has important reference value and significance for the research of cloud service trustworthiness assessment.
Metal Dusting Behaviour of Fe-Cr and Fe-Cr-Ni Alloys under Blast Furnace Operating Condition
Authors: Murakami Tatsuya , Nguyen Thuan Dinh , Xi Xuteng , Zhang Jianqiang 
Published: March 2023

Abstract
Metal dusting behaviours of Fe-(10, 20, 25)Cr, Fe-20Cr-(20, 33)Ni, Fe-25Cr-20Ni (wt.%) in a Ar-33CO-7H2-7CO2-2H2O (vol.%) gas at 550℃ and 650℃ were investigated in simulating a blast furnace operating condition. Severe metal dusting and coke deposition were observed at 550℃, while no significant dusting but internal oxidation and carburization were observed at 650℃. Higher Cr and Ni contents in the alloy lowered kinetics of metal dusting at 550℃ and of carburization at both temperatures. In particular, Fe-25Cr was the only alloy exhibiting no significant metal dusting at 550℃. Increasing Cr concentration also reduced oxidation kinetics at both temperatures. Austenitic alloys were more susceptible to metal dusting and coke deposition than ferritic alloys with the same Cr content at 550℃. At 650℃, however, ferritic alloys experienced higher extents of carburization and oxidation than austenitic alloys with the same Cr concentration.
First report of Bovine Viral Diarrhea Virus subgenotypes 1d and 1e in Southern Chile
Authors: Hugues Florence , Cabezas Ignacio , Garigliany Mutien , Rivas Felipe , Casanova Tomás , González Eddy E. , Sánchez Oliberto , Castillo Raúl , Parra Natalie C. , Inostroza-Michael Oscar , Moreno Lucila , Hernández Cristián E. , Toledo Jorge R. 
Published: March 2023

AbstractBovine Viral Diarrhea virus (BVDV) affects cattle worldwide causing severe productive and economic loss. In this study, we investigated the subgenotypes of BVDV circulating in cattle samples from the Aysén region, an active cattle breeding area located in southern Chile. Partial amplification of the 5' untranslated region (UTR) was performed by polymerase chain reaction (PCR), and twelve samples were analyzed by Sanger sequencing and phylogenetic analysis. Eight samples were identified as belonging to BVDV-1e, three to 1-b, and one to 1-d. The phylogenetic analyses performed revealed a marked distance between these now-identified strains and those previously reported in the country. These findings support the need to continually expand the analysis of the variability of the viral phylogeny for the currently circulating BVDV strains and to update the vaccines recommended for this livestock area and surrounding areas.
Challenges During Temperature Mapping Process in Pharmaceutical Products Warehouses in Tanzania.
Authors: Mvandal Samson Peter , Haji Msema 
Published: March 2023

Abstract
Pharmaceutical product generally depends on environment controls during its storage and handling. Temperature mapping is essential and should be managed during entire product lifecycle with holistic approach. The purpose of this study was to determine the challenges during temperature mapping process in pharmaceutical product warehouses in Tanzania. The task analysis of temperature mapping process was carried out. As a result of the study, general requirements were formulated for the preparation; challenges were identified. Study shows majority of pharmaceutical workers lack of knowledge on temperature mapping, the use of local tool in temperature affecting the efficacy, safety and efficiency of the pharmaceutical product. The frequency of temperature mapping envisaged unsatisfactory. Hence, temperature mapping process in pharmaceutical industry should be recorded and reported to the manufacturer for further investigation and risk analysis. To address these challenges, implementation of a Quality Management System (QMS) must emphasize on the importance of temperature mapping and provides guidelines for its proper handling. The QMS should be integrated into the pharmaceutical warehouse management system and should involve all pharmaceutical workers, including medicine distributors and consumers. The concept of temperature mapping process is a critical that should be well-understood and implemented by pharmaceutical workers in pharmaceutical industry, through following proper guidelines and integrating QMS, and the challenges associated with temperature mapping can be minimized, and the quality of pharmaceutical products can be ensured.
Diastolic Dysfunction Criteria and Heart failure Readmission in Patients with Heart Failure and Reduced Ejection Fraction
Authors: Lavine Steven J. , Kelvas Danielle 
Published: March 2023

Background:
Advanced diastolic dysfunction (DDys) correlates with elevated LV filling pressures and predicts heart failure readmission (HF-R). As grade 2-3 DDys has predictive value for HF-R, and requires 2 of 3 criteria (left atrial volume index >34 ml/m2, E/e’>14, or tricuspid regurgitation velocity >2.8 m/s), we hypothesized that all 3 criteria would predict greater HF risk than any 2 criteria.

Methods:
In this single-center retrospective study that included 380 patients in sinus rhythm with HF and reduced ejection, we recorded patient characteristics, Doppler-echo, and HF-R with follow-up to 2167 days (median=1423 days; interquartile range=992-1821 days).

Results:
For grade 1 DDys (223 patients), any single criteria resulted in greater HF-R as compared to 0 criteria (HR=2.52, (1.56-3.88) p<0.0001) with an AUC (area under curve)=0.637, p<0.001. For grade 2 DDys (94 patients), there was greater HF-R for all 3 (vs. 0 criteria: HR=4.0 (2.90-8.36), p<0.0001). There was greater HF-R for 3 vs any 2 criteria (HR=1.81, (1.10-3.39), p=0.0222). For all 3 criteria, there was moderate predictability for HF-R (AUC=0.706, p<0.0001) which was more predictive than any 2 criteria (AUC difference 0.057, (0.011-0.10), p=0.009). For grade 3 DDys (63 patients), E/A>2+2-3 criteria identified a subgroup with the greatest risk of HF-R (HR=5.03 (4.62-22.72), p<0.0001) compared with 0 DDys criteria with moderate predictability for 2-3 criteria (AUC=0.726, p<0.0001) exceeding E/A>2+0-1 criteria (AUC difference=0.120, (0.061-0.182), p<0.001).

Conclusion:
Increasing the number of abnormal criteria increased the risk and predictive value of HF-R for grade 1-3 DDys in patients with HF with reduced ejection fraction.
Spatiotemporal and Seasonal Trends of Class A and B Notifiable Infectious Diseases in China: Retrospective Analysis
Authors: Zheng Junyao , Zhang Ning , Shen Guoquan , Liang Fengchao , Zhao Yang , He Xiaochen , Wang Ying , He Rongxin , Chen Wenna , Xue Hao , Shen Yue , Fu Yang , Zhang Wei-Hong , Zhang Lei , Bhatt Samir , Mao Ying , Zhu Bin 
Published: March 2023

Background
China is the most populous country globally and has made significant achievements in the control of infectious diseases over the last decades. The 2003 SARS epidemic triggered the initiation of the China Information System for Disease Control and Prevention (CISDCP). Since then, numerous studies have investigated the epidemiological features and trends of individual infectious diseases in China; however, few considered the changing spatiotemporal trends and seasonality of these infectious diseases over time.

Objective
This study aims to systematically review the spatiotemporal trends and seasonal characteristics of class A and class B notifiable infectious diseases in China during 2005-2020.

Methods
We extracted the incidence and mortality data of 8 types (27 diseases) of notifiable infectious diseases from the CISDCP. We used the Mann-Kendall and Sen’s methods to investigate the diseases’ temporal trends, Moran I statistic for their geographical distribution, and circular distribution analysis for their seasonality.

Results
Between January 2005 and December 2020, 51,028,733 incident cases and 261,851 attributable deaths were recorded. Pertussis (P=.03), dengue fever (P=.01), brucellosis (P=.001), scarlet fever (P=.02), AIDS (P<.001), syphilis (P<.001), hepatitis C (P<.001) and hepatitis E (P=.04) exhibited significant upward trends. Furthermore, measles (P<.001), bacillary and amebic dysentery (P<.001), malaria (P=.04), dengue fever (P=.006), brucellosis (P=.03), and tuberculosis (P=.003) exhibited significant seasonal patterns. We observed marked disease burden–related geographic disparities and heterogeneities. Notably, high-risk areas for various infectious diseases have remained relatively unchanged since 2005. In particular, hemorrhagic fever and brucellosis were largely concentrated in Northeast China; neonatal tetanus, typhoid and paratyphoid, Japanese encephalitis, leptospirosis, and AIDS in Southwest China; BAD in North China; schistosomiasis in Central China; anthrax, tuberculosis, and hepatitis A in Northwest China; rabies in South China; and gonorrhea in East China. However, the geographical distribution of syphilis, scarlet fever, and hepatitis E drifted from coastal to inland provinces during 2005-2020.

Conclusions
The overall infectious disease burden in China is declining; however, hepatitis C and E, bacterial infections, and sexually transmitted infections continue to multiply, many of which have spread from coastal to inland provinces
Combining Outreach and Cross-sectional Research to Gather Children’s Soil Values in Aotearoa New Zealand: Protocol for the Mixed Methods Soilsafe Kids Program
Authors: Tsang Sophia , Egli Victoria , Crawshaw-Mclean Bridget , Edwards-Maas Lianne , Kah Melanie , Sharp Emma 
Published: March 2023

Background
Soil underpins most terrestrial systems; hence, its degradation should concern everyone. In 2021, Soilsafe Aotearoa surveyed the adult population of New Zealand about how they value soil, particularly values related to how they care about and are concerned about soil. Pursuant to this study, Soilsafe Kids (the outreach branch of Soilsafe Aotearoa) developed a combined research and outreach program to collect a supplemental data set of children’s soil values, so both adults’ and children’s voices can be considered when understanding the implications of different practices and how to care for presently “uncared for” or neglected soils in the future.

Objective
The program not only asks primary school students about their soil values but also aims to teach them about soil from many disciplinary perspectives to enhance their understanding and awareness of soil, and, more broadly, for knowledge production.

Methods
Here we describe the research protocol used in this Soilsafe Kids program. This program uses surveys (in the form of worksheets), focus groups (introduced as group discussions), and art projects to learn what children think about soil in Tāmaki Makaurau Auckland. We have received ethics approval from the University of Auckland’s Human Participants Ethics Committee (reference number 23556) on March 25, 2022, for 3 years.

Results
We have begun recruiting participants and delivering the Soilsafe Kids program in schools across Tāmaki Makaurau Auckland. Our data collection is ongoing with final student engagement in the first quarter of 2023. We expect to analyze data at the start of 2023 and to disseminate results later this year.

Conclusions
Once this study is complete, we will disseminate the final results to the research community, stakeholders, and the local community through conference presentations, journal articles, hui (meetings), on our website, and in art exhibits. We note that although Tāmaki Makaurau Auckland is home to the majority of people living in Aotearoa New Zealand, the Auckland region only represents a small portion of Aotearoa New Zealand’s land, and findings are not generalizable to Aotearoa New Zealand as a whole.

International Registered Report Identifier (IRRID)
DERR1-10.2196/43390