bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chorionicity-associated variation in metabolic phenotype of cord blood in twin
Authors: Liu Xiaoyu , Yang Jing , Ran Rui , Long Fei , Yang Yang , Dong Xiaojing , Saffery Richard , Novakovic Boris , Mousa Hatem , Wei Yuan , Hu Lina , Han Ting-Li 
Published: July 2023

Abstract
Background
Monochorionic (MC) twins present a higher incidence of unfavorable clinical perinatal outcomes than dichorionic (DC) twins, often in association with placental vascular anastomosis. In this study, we profiled the umbilical cord plasma metabolomes of uncomplicated MC and DC twin pregnancies and related these to several offspring outcomes, previously associated with birthweight.

Methods
Umbilical vein blood samples were collected at birth from 25 pairs of uncomplicated MC twins and 24 pairs of uncomplicated DC twins. The samples were subjected to gas chromatography-mass spectrometry-based metabolomics. 152 metabolites were identified from the cord plasma samples of MC and DC twins. Partial least squares discriminant analysis and pathway analysis were performed to compare within DC/MC twin pairs and between DC and MC twins. A generalized estimating equation (GEE) model was utilized to explore the correlation between metabolic differences and birthweight discordance within and between twin pairs.

Results
Our study revealed clear differences between the metabolite profiles of umbilical cord plasma of MC and DC twins. Metabolite profiles in MC within twin pairs and DC within twin pairs were characterized by the differences in 2 − hydroxyglutaramic acid levels and nicotinamide levels, respectively. The metabolic pathways of GSH, tryptophan, and fatty acid metabolism, were significantly downregulated in MC twins compared to DC twins. In addition, the concentration of caffeine and decamethyl-cyclopentasiloxane (D5) was positively correlated with birthweight in MC and DC twins.

Conclusion
This study demonstrated that the altered metabolites in umbilical plasma made contributions to the different chorionicities between uncomplicated MC twins and DC twins. The chorionicity of twins seems to affect the metabolic cross-talk between co-twin pairs and be related to birthweight discordance of twins.
Pediatric unit spending in the North of Italy during the COVID-19 pandemic
Authors: Franceschi Roberto , Maines Evelina , Petrone Angelamaria , Bilato Simone , Trentini Ilaria , Di Spazio Lorenzo , Leonardi Luca , Soffiati Massimo , Francesconi Andrea 
Published: July 2023

Abstract
Background
During the COVID-19 pandemic, accesses to pediatric health care services decreased, as well as the consumption of traditional drugs, while the median cost per patient at the emergency department slightly increased and the cost of pediatric COVID-19 admissions to the pediatric ward too. Overall spending of a secondary level Pediatric Unit in the last two years has not been previously reported.

Methods
This is a retrospective study conducted by the Pediatric Unit of S. Chiara Hospital of Trento, North of Italy. We collected data on consumption and spending before and during the COVID-19 pandemic (between January 2018 and December 2022).

Results
The total spending ranged from 2.141.220 to 2.483.931 euros between 2018 and 2022. COVID-19 spending accounted only for 5–8% of the overall budget, while two macro-areas of spending were identified: (i) biologic drugs for inherited metabolic diseases (IMDs), that impacted for 35.4–41.3%, and (ii) technology devices for type 1 diabetes (T1D), that accounted for 41.6–32.8% of the overall budget, in 2021 and 2022, respectively. Analysis of costs along with the different health care services revealed that: (i) the spending for COVID-19 antigen tests and personal protective equipment had a major impact on the Emergency room budget (from 54 to 68% in the two years); (ii) biological drugs accounted mainly on the Pediatric Ward (for 57%), Day Hospital (for 74%) and rare disease center budget (for 95% of the spending); (iii) the cost for T1D devices was mainly due to continuous glucose monitoring, and impacted for the 97% of the outpatient clinic budget.

Conclusions
The main impact on the budget was not due to COVID-19 pandemic related costs, but to the costs for biologic drugs and T1D devices. Therefore, cost savings could be mainly achieved through generic and biosimilars introduction and with inter-regionals calls for technology devices. We emphasize how the control of spending in pediatric hospital care has probably moved from the bedside (savings on traditional drugs as antibiotics) to the bench of national or inter-regional round tables, to obtain discounts on the costs of biologic drugs and medical devices. Here we provide for the first-time in literature, data for bench-marking between secondary level Pediatric Units before and during the COVID-19 pandemic.
Association of air pollution with postmenopausal breast cancer risk in UK Biobank
Authors: Smotherman Carmen , Sprague Brian , Datta Susmita , Braithwaite Dejana , Qin Huaizhen , Yaghjyan Lusine 
Published: July 2023

Abstract
Background
We investigated the association of several air pollution measures with postmenopausal breast cancer (BCa) risk.

Methods
This study included 155,235 postmenopausal women (of which 6146 with BCa) from UK Biobank. Cancer diagnoses were ascertained through the linkage to the UK National Health Service Central Registers. Annual exposure averages were available from 2005, 2006, 2007, and 2010 for NO2, from 2007 and 2010 for PM10, and from 2010 for PM2.5, NOX, PM2.5–10 and PM2.5 absorbance. Information on BCa risk factors was collected at baseline. Cox proportional hazards regression was used to evaluate the associations of year-specific and cumulative average exposures with BCa risk, overall and with 2-year exposure lag, while adjusting for BCa risk factors.

Results
PM10 in 2007 and cumulative average PM10 were positively associated with BCa risk (2007 PM10: Hazard ratio [HR] per 10 µg/m3 = 1.18, 95% CI 1.08, 1.29; cumulative average PM10: HR per 10 µg/m3 = 1.99, 95% CI 1.75, 2.27). Compared to women with low exposure, women with higher 2007 PM10 and cumulative average PM10 had greater BCa risk (4th vs. 1st quartile HR = 1.15, 95% CI 1.07, 1.24, p-trend = 0.001 and HR = 1.35, 95% CI 1.25, 1.44, p-trend < 0.0001, respectively). No significant associations were found for any other exposure measures. In the analysis with 2-year exposure lag, both 2007 PM 10 and cumulative average PM10 were positively associated with BCa risk (4th vs. 1st quartile HR = 1.19, 95% CI 1.10, 1.28 and HR = 1.29, 95% CI 1.19, 1.39, respectively).

Conclusion
Our findings suggest a positive association of 2007 PM10 and cumulative average PM10 with postmenopausal BCa risk.
A quick, easy and efficient protocol for extracting high-quality RNA from Mycobacterium tuberculosis using a spin column commercial kit
Authors: Mvubu NE , Salig A. , Moopanar K. , Nyide ASG , Govender D. , Mankayi E. 
Published: July 2023

AbstractRNA extraction from Mycobacterium tuberculosis has been a historically challenging task for researchers due to the thick lipids associated with the cell wall of this “notorious” pathogen that is responsible for Tuberculosis (TB) outbreaks. Several studies have successfully extracted RNA from M. tuberculosis using a Trizol reagent combined with organic solvents. Recently, our laboratory has successfully extracted high quality total RNA using a commercial kit from clinical strains belonging to F15/LAM4/KZN, Beijing and F11 strain families and H37Rv laboratory strain by exploiting high speed homogenizer for cell lysis and spin columns for RNA purification. The quality and integrity of the extracted RNA was analyzed and confirmed through the Nanodrop, Bioanalyzer and RNA 3-(N-morpholino) propanesulfonic acid (MOPS) gel electrophoresis. Furthermore, to confirm the integrity of small RNA (sRNA) molecules due to their vulnerability to degradation, the RNA samples were converted to cDNA and sRNAs were amplified and confirmed through PCR. This detailed RNA extraction protocol proposes to carve a new path into TB transcriptome research without the use of organic solvent for downstream purification steps while yielding high quality RNA that can be used to understand M. tuberculosis transcriptome regulation.
Bibliometric and visual analysis of diabetes mellitus and pyroptosis from 2011 to 2022
Authors: Li Xiaodong , Su Xiaojuan , Xia Fenglin , Qiu Jing , Zhang Jiaqi , Wu Haiyan , Xie Xuejun , Xu Mingchao 
Published: July 2023

AbstractObjectiveTo visualize and analyze the published literature on diabetes mellitus and pyroptosis based on a bibliometric approach, so as to provide a comprehensive picture of the hot research directions and dynamic progress in this field.MethodsThis study was based on the web of science core collection database to conduct a comprehensive search of the published literature in the field of diabetes mellitus and Pyroptosis from January 1985 to August 2022, including the published research literature in this field, as well as a visual analysis of the number of citations, year of publication, journal, author, research institution, country, and research topic.ResultsA total of 139 literature on research related to diabetes mellitus and cellular scorch from 2011 to 2022 were retrieved, with a total of 3009 citations and a maximum of 255 citations for a single article, which had a first author Schmid-Burgk, JL The first author of this article is from Germany; among 20 publishing countries, China leads with 100 articles; among 222 publishing institutions, Harbin Medical University leads with 18 articles and 184 citations; among 980 authors, Chen, X from China tops the list of high-impact authors with 5 articles and 29 citations. Among the 98 journals, "CELL DEATH DISEASE" ranked first in both volume and high-impact journals with 4 articles and 29 citations. Among 349 keywords, "pyroptosis" ranked first with a cumulative frequency of 65 times. The cluster analysis was divided into three categories, chronic complications of diabetes mellitus and pyroptosis (67 articles), diabetes mellitus and pyroptosis (60 articles), and diabetes mellitus combined with other diseases and pyroptosis (12 articles), and the number of articles related to diabetes mellitus and its chronic complications increased rapidly from 2019, among which, diabetic cardiomyopathy (27 articles) had the highest number of articles.ConclusionsBased on a comprehensive analysis of published literature in the field of diabetes mellitus and pyroptosis from 2011 to 2022, this study achieved a visual analysis of studies with significant and outstanding contributions to the field, thus framing a picture showing the development and changes in the field. At the same time, this study provides research information and direction for clinicians and investigators to conduct diabetes mellitus and pyroptosis-related research in the future.
Stereotactic radiotherapy for brain metastases: predictive factors of radionecrosis
Authors: Calderon Benoît , Vazquez Léa , Belkacemi Mohammed , Pourel Nicolas 
Published: July 2023

Abstract
Purpose
Stereotactic radiotherapy (SRT) is a highly effective approach and represents the current standard of treatment for patients with limited number of brain metastasis (BM). SRT is generally well tolerated but can sometimes lead to radionecrosis (RN). The aim of this study was to identify predictive factors of radionecrosis related to SRT for brain metastasis.

Methods
This retrospective observational cohort study included patients who underwent SRT in the Institut Sainte Catherine between January 1st, 2017 and December 31st, 2020 for the treatment of brain metastasis from any cancer. Individual data and particularly signs of radionecrosis (clinical, imaging, anatomopathological) were collected from electronic medical records. Radionecrosis was defined as the occurrence on MRI of contrast-enhancing necrotic lesions, surrounded by edema, occurring at least 6 months after SRT and localized within fields of irradiation.

Results
123 patients were included; median age was 66 years. 17 patients (11.8%) developed radionecrosis after a median follow up of 418.5 days [63;1498]. Predictive factors of radionecrosis in multivariate analysis were age under 66 years with a sensitivity of 77% and a specificity of 56%. No other factor as the presence of comorbidities, the number of irradiated metastases, the PTV volume or the volume of irradiated healthy brain were predictive of radionecrosis.

Conclusion
Age at treatment initiation and tumor location seems to be correlated with radionecrosis in patients with brain metastasis treated with SRT. These elements could be useful to adapted radiation therapy.
In-person and online sensory wellbeing workshop for eating disorders: updated case series
Authors: Li Zhuo , Holetic Victoria , Webb Jessica , Chubinidze Dimitri , Byford Sarah , Tchanturia Kate 
Published: July 2023

Abstract
Background
A one-off sensory wellbeing workshop has been developed to help patients with eating disorders (ED) manage sensory sensitivities. The aim of this study was to evaluate and compare the outcomes of the workshop in online versus face-to-face (F2F) formats among a sample of patients with ED.

Methods
Cumulative link models were applied to the outcome measures (awareness of sensory wellbeing, awareness of strategies to enhance sensory wellbeing, and confidence in managing sensory wellbeing) to test the differences between online and F2F workshops. Participants’ ratings of usefulness of the workshop were also compared between online and F2F workshops.

Results
A total of 14 workshops (4 online and 10 F2F) were run from 2020 to 2023. All participants reported significant and substantial improvements in all outcome measures. There was no significant difference in outcomes between online and F2F workshops. The majority of patients rated the workshops as useful.

Conclusions
Both online and face-to-face formats of the sensory workshop led to improvement in sensory wellbeing management for patients with ED. Future studies are warranted to test the impact of the workshop on ED treatment outcomes.
Nondestructive characterization and artificial intelligence recognition of acoustic identifiers of ancient ceramics
Authors: Jin Xiaoxue , Wang Xiufeng , Xue Chaohua 
Published: July 2023

Abstract
Cultural heritage identity management is the most basic and important work in the process of cultural heritage protection. It is of great significance to provide a unique and identifiable digital identity for ancient ceramics. At present, the identification information of ancient ceramics is mainly composed of external visual characteristics, and there is no report on feature identification method that can reflect the properties of ancient ceramics. Audible sound signals not only have advantages in non-destructive testing, but also can be used as voiceprint information to identify, monitor and analyze ancient ceramics. In this paper, seven ancient ceramics and 12 similar modern ceramic cups are taken as research objects, and an acoustic identifier (AID) is constructed. We put forward a reliable acoustic identification method for ancient ceramics, and established a digital code of acoustic characteristics of ancient ceramics. The results show that audible sound waves can reflect the attribute information of ancient ceramics. Sufficient acoustic data combined with deep learning can not only accurately match the identity of ancient ceramics, but also detect the real-time identity information of ancient ceramics, and make a comparative analysis of its cracks and whether it has caused damage. This method can provide a variety of practical applications for audible signal feature recognition technology in the exhibition, protection, trading, recognition and safety management of ancient ceramics and other cultural relics.
A macroscopic analysis of the demographic impacts of flood inundation in Thailand (2005–2019)
Authors: Tsuda Hinako , Tebakari Taichi 
Published: July 2023

AbstractIn Thailand, floods are occurring more frequently due to climate change, and recent economic development and population growth may have altered the way in which people interact with floods, including migration to other regions. In this study, we analyzed the relationship between flooding and population distribution across Thailand from 2005 to 2019 to improve measures for minimizing flood damage. We used population distribution point data from 2009 to 2019 produced by Oak Ridge National Laboratory to analyze trends in population movement and distribution, by examining whether population sizes were greater than, less than, or equal to estimated values in regions throughout Thailand. The results suggest that floods in 2011 and 2017 caused temporary migration to areas that were not inundated or to the metropolitan Bangkok area. Flood responses changed after the 2011 floods, which have been described as the worst flood in Thai history. Next, we examined the relationship between the number of regions with lower than estimated population and flood data for the previous year including precipitation, inundated area, and deaths caused by flooding. Inundation area had a significant impact on population decline, with correlation coefficients of 0.426 and 0.501 for the north and northeast, respectively. The number of deaths caused by flooding in a given year also led to a population decline in the following year. However, precipitation did not exhibit the same trend. Therefore, population demographics after floods have shown regional characteristics in recent years, with Thai people shifting from a flood-tolerance lifestyle to a flood-avoidance lifestyle, mainly in local urban areas and the metropolitan Bangkok area.
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice
Authors: Lim YoonHee , Park So-Hyun , Kim Eun Jae , Lim HeeJun , Jang Jinsun , Hong In-Sun , Kim Sanghee , Jung YunJae 
Published: July 2023

Abstract
Background
Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first line of defense against external threats, polar microalgae extracts may possess restorative properties for damaged skin, but the potential of microalgae extracts as skin protective agents remains unknown.

Purpose
This study aimed to analyze compound profiles from polar microalgae extracts, evaluate their potential as skin epithelial protective agents, and examine the underlying mechanisms.

Methods
Six different polar microalgae, Micractinium sp. (KSF0015 and KSF0041), Chlamydomonas sp. (KNM0029C, KSF0037, and KSF0134), and Chlorococcum sp. (KSF0003), were collected from the Antarctic or Arctic regions. Compound profiles of polar and non-polar microalgae extracts were analyzed using gas chromatography-mass spectrometry (GC-MS). The protective activities of polar microalgae extracts on human keratinocyte cell lines against oxidative stress, radiation, and psoriatic cytokine exposure were assessed. The potential anti-inflammatory mechanisms mediated by KSF0041, a polar microalga with protective properties against oxidative stress, ultraviolet (UV) B, and an inflammatory cytokine cocktail, were investigated using RNA-sequencing analysis. To evaluate the therapeutic activity of KSF0041, an imiquimod-induced murine model of psoriatic dermatitis was used.

Results
Polar microalgae contain components comparable to those of their non-polar counterparts, but also showed distinct differences, particularly in fatty acid composition. Polar microalgae extracts had a greater ability to scavenge free radicals than did non-polar microalgae and enhanced the viability of HaCaT cells, a human keratinocyte cell line, following exposure to UVB radiation or psoriatic cytokines. These extracts also reduced barrier integrity damage and decreased mRNA levels of inflammatory cytokines in psoriatic HaCaT cells. Treatment with KSF0041 extract altered the transcriptome of psoriatic HaCaT cells toward a more normal state. Furthermore, KSF0041 extract had a therapeutic effect in a mouse model of psoriasis.

Conclusions
Bioactive compounds from polar microalgae extracts could provide novel therapeutics for damaged and/or inflamed skin.
Recovery of memory retention after anesthesia with remimazolam: an exploratory, randomized, open, propofol-controlled, single-center clinical trial
Authors: Nobukuni Keiko , Shirozu Kazuhiro , Maeda Aiko , Funakoshi Kouta , Higashi Midoriko , Yamaura Ken 
Published: July 2023

Abstract
Purpose
Remimazolam, a newly developed ultra-short-acting benzodiazepine, provides early recovery of consciousness but its effects on memory recovery are unclear. This study examined memory recovery after emergence from general anesthesia using remimazolam.

Methods
Seventy-four patients undergoing breast surgery between October 2021 and March 2022 were enrolled and randomly assigned to receive propofol (control group) or remimazolam as general anesthetic during surgery. The primary endpoint was the number of posters patients remembered 24 h after surgery (among four posters shown after recovering from anesthesia) as an assessment of memory retention. The secondary endpoints were the recall of a numeric character patients had been shown just before anesthetic induction, as an assessment of retrograde amnesia 24 h after surgery.

Results
Sixty-six patients (propofol, 32; remimazolam, 34) were assessed. Patients in the remimazolam group remembered significantly fewer posters shown to them after surgery than those in the propofol group (0 [0 − 2] vs. 2 [1 − 3], p < 0.001). In the remimazolam group, the patients who received flumazenil remembered a higher number of posters than those who did not receive flumazenil (3 [1 − 4] vs. 0 [0 − 0], p < 0.001). All patients remembered all events that occurred during the preoperative period as well as the numeric character.

Conclusion
Patients recovering from remimazolam anesthesia without receiving flumazenil do not remember events after regaining consciousness.

IRB
Kyushu University School of Medicine Hospital Institutional Review Board (IRB) (approval number: 20212006).

Trial registration
This clinical trial was registered with the University Hospital Medical Information Network (UMIN) Center on September 28, 2021 (UMIN-CTR: UMIN000045593).

Implication statement
Memory recovery is slower following emergence from remimazolam than from propofol anesthesia.