bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncovering the biocontrol mechanism of Bacillus subtilis YPS-32 antagonism of Streptomyces scabies infection through transcriptome and proteome analysis
Authors: Zhou Yingjun , li qing , Peng Zheng , Li Jianghua , Zhang Juan 
Published: January 2023

Abstract
Background
The bacterium Streptomyces scabies is the main pathogen causing potato common scab. Efforts to control S. scabies infection using the bacterial strain Bacillus subtilis YPS-32 as a biocontrol agent have been excellent; however, the underlying biocontrol mechanism is not clear.
Results
In this study, we first determined the key time points of B. subtilis YPS-32 inhibition of S. scabiesgrowth by analyzing the interaction of the two bacterial species in co-culture. We then analyzed differential changes in gene and protein expression patterns in B. subtilis YPS-32 after 0 h (control) and 24 h (peak inhibition) co-culture, using transcriptomic and proteomic sequencing, respectively. We found that the expression levels of genes and proteins related to the synthesis of the antimicrobial metabolites fengycin, bacillaene, and bacilysin were significantly up-regulated in B. subtilis YPS-32 after 24 h co-culture. In addition, the protein, WP_003245811.1 related to bacillibactinexport was also upregulated in co-culture.
Conclusions
The fengycin, bacillaene, bacilysin and bacillibactin were potential antibacterial metabolites of B. subtilis YPS-32.This research will provide support for the further molecular mechanism research of B. subtilis YPS-32 metabolites.
Acid-driven immune suppression by pHLIP-fused PD-L1 under inflammatory conditions
Authors: Chen Guojiang , Zheng Hang , Zhang Yuting , Wu Haiyan , Feng Junjuan , Sun Ying , Zhang Min , Xiao He , Qiao Chunxia , Wang Jing , Luo Longlong , Li Xinying , Feng Jiannan , Zheng Yuanqiang , Wang Yi , Shi Yanchun 
Published: January 2023

Abstract
Programmed cell death–ligand 1 (PD-L1)/PD-1 axis is crucial for maintenance of immune homeostasis and its impairment partially accounts for the pathogenesis of inflammatory diseases. Hence, augmenting PD-L1/PD-1 signals represents a novel strategy to prevent destructive inflammation and induce immune tolerance. Recently, we developed a new cargo by conjugating the ectodomain of PD-L1 with pHLIP, a low pH-responding and membrane-inserting peptide, and demonstrated its potent immune-suppressive activity under acidic conditions in vitro. Herein, we further showed that PD-L1-pHLIP well responded to relatively high acidic buffer, while it could not inhibit T cell expansion in weakly acidic solutions. Furthermore, in a mouse model of acute intestinal inflammation, PD-L1-pHLIP treatment prolonged survival time and attenuated colitis in mice subjected to 6% dextran sulfate sodium (DSS) instead of 3% DSS. The different efficacy was due to the distinct acidity in the lesions, which facilitated PD-L1-pHLIP accumulation in the niche of 6% DSS-triggered severe inflammation. Mechanistic investigations revealed that PD-L1-pHLIP inhibited the release of proinflammatory cytokines in infiltrating macrophages and other immune cells in a PD-1-dependent and/or –independent fashion. Taken together, this study highlights PD-L1-pHLIP as a novel therapeutic avenue for inflammatory diseases.
Autophagy-related genes and pathways was associated with osteoarthritis pathogenesis
Authors: Guan Taiyuan , Guo Yang , Wu Chengjie , Niu Yuanyuan , Xiao Jirimutu , Ma Yong 
Published: January 2023

Abstract
Background: Patients with osteoarthritis have musculoskeletal-related chronic disability, leading to the high pain intensity. Explaining the molecular mechanisms of osteoarthritis is critial for the diagnosis and cure. Therefore, This research aimed to find key candidate genes involved in osteoarthritis pathogenesis.
Methods: We identified differentially expressed genes by integrating multiple microarry datasets in cartilage (GSE43923, GSE113825, GSE129147 and GSE169077). Functional enrichment analysis and protein-protein interaction analysis were performed.
Results: We identified sixty-six significantly expression genes (56 up-regulated and 10 down-regulated). Through functional enrichment analysis and protein-protein interaction analysis, we found that the biological process of these genes was enriched in focal adhesion, ECM-receptor interaction and PI3K-Akt signaling, which were closely related with autophagy. Moreover, ceRNA network showed that thirty-four DEGs, including ECM-receptor interaction-related genes (COL4A1, COL4A2 and COL1A2, LAMB1 an THBS2), exist competing endogenous regulating network mediated by 7 lncRNAs and 8 miRNAs. Furthermore, differentially expressed autophagy-related genes (CCL2, CDKN1A, CXCR4, DAPK1, DLC1, FAS, HSPA8, MYC and SERPINA1) were remarkably identified to interact with multiple of the common DEGs in ECM-receptor interaction and PI3K-Akt signaling pathway, suggesting that autophagy plays important role in osteoarthritis pathogenesis by regulating ECM-receptor interaction and PI3K-Akt signaling pathway.
Conclusions: This multiple transcriptome analysis identifies ECM-receptor interaction and PI3K-Akt signaling pathway related to osteoarthritis pathogenesis by regulating autophagy and participating in ceRNA network.
Identifying Cultivation Hotspots of Aquilegia fragrans Benth.-an Endangered Medicinally Important Plant via Ensemble Modelling under Climate Change Scenarios
Authors: Bhat Irshad Ahmad , Fayaz Mudasir , -ul-Qadir Roof , Rafiq Shah , Guleria Khushboo , Qadir Jasfeeda , wani Tareq A , Kaloo Zahoor A 
Published: January 2023

Abstract
Climate change is one of the main drivers for species redistribution and biodiversity loss, especially for endemic and medicinally important plant species with a restricted distributional range. For that reason, it is vital to comprehend "how" and "where" priority medicinal and aromatic plants (MAPs) might be effectively used to address conservation-related issues under rapid climate change. In the current study, we used an ensemble modelling approach to investigate the present and future potential distribution coupled with the range dynamics of the Aquilegia fragrans–a medicinally important endangered plant species in the entire spectrum of the Himalayan biodiversity hotspot. The findings of the current study revealed that, under current climatic conditions, the northwest states of India (Jammu and Kashmir, Himachal Pradesh, and the northern part of Uttarakhand); the eastern and southern parts of Pakistan Himalaya have highly suitable and optimal climatic conditions for the growth of A. fragrans. The ensemble model exhibited high forecast accuracy, with temperature seasonality (BIO4) and precipitation seasonality (BIO15) as the main climatic variables responsible for the distribution in the biodiversity hotspot. Furthermore, the study predicted that future climate change scenarios will diminish habitat suitability for the species by -46.922% under RCP4.5 2050 and − 55.052% under RCP4.5 2070. Likewise, under RCP8.5 the habitat suitability will decrease by -51.760% in 2050 and-94.351% in 2070. The current study also revealed that the western Himalayan area will show the most habitat loss. Some regions that are currently unsuitable such as the northern Himalayan regions of Pakistan will become more suitable under climate change scenarios. Hopefully, the current approach will provide a robust technique and showcases a model with learnings for predicting cultivation hotspots and devising scientifically sound conservation plans for this endangered medicinal plant in the Himalayan biodiversity hotspot.
Multidetector computed tomography angiography findings predicts the need for bronchial artery embolization for moderate to severe hemoptysis: a single-center retrospective cohort study
Authors: Sakuranaka Haruyasu , Nakayama Ryouta , Yamakawa Yuji , Nakamoto Tadaharu , Igei Komei , Okamoto Naoki , Asai Yasuo , Ichioka Masahiko 
Published: January 2023

Abstract
Background
While the utility of multidetector computed tomography angiography (MDCTA) as a guide before bronchial artery embolization (BAE) has been demonstrated, its utility in determining the need for BAE is unclear. In the present study, we investigated whether MDCTA could identify the need for BAE in patients with moderate to severe hemoptysis.
Methods
This single-center retrospective cohort study included 32 patients with moderate to severe hemoptysis > 100 mL/day requiring hospitalization from April 1, 2012 to March 31, 2015. The relationship between MDCTA findings and the need for BAE was analyzed.
Results
MDCTA revealed normal bronchial arteries in 14 of the 32 patients. Among the remaining 18 patients with abnormally dilated bronchial arteries, the arteries remained within the mediastinum in 6 patients and extended from the mediastinum to the pulmonary parenchyma in 12 patients. None of the six patients’ bronchial arteries that remained within the mediastinum underwent BAE, regardless of the dilation status, and hemoptysis could be controlled with conservative treatment. BAE was required in 58% (7/12) of the patients with abnormally dilated bronchial arteries extending to the pulmonary parenchyma. There was a significant association between MDCTA findings and the need for BAE (p < 0.001).
Conclusions
MDCTA finding of abnormally dilated bronchial artery extending from the mediastinum to the pulmonary parenchyma may predict the need for BAE in patients with moderate to severe hemoptysis.
Assessment of Urban Expansion of Jaipur City and Its Impact on UHI With Respect to Lst Using Geoinformatics
Authors: Kumar Rajesh , Jalem Kiran , Singh Harikesh 
Published: January 2023

Abstract
The spread of urban development is now related to the expansion of human and social development. Rapid urbanization is also causing an increase in urban temperature, which creates Urban Heat Island (UHI) and reduces vegetation space, so it is critical to monitor urban expansion and Land Surface Temperature (LST) to prevent the city's temperature from rising further. The current analysis focuses on Jaipur's urban development over the last 16 years. The change was influenced by the LANDSAT 7 ETM+ (2000, 2008 and 2016) satellite symbology. According to the findings, the city has experienced rapid changes in land use, particularly in terms of built-up area, which has nearly doubled in the last 16 years. The study also attempted to examine the impact of urbanization on UHI in relation to LST by employing MODIS data. The geographical study of Jaipur city is performed in order to determine the correlations between urban expansion rate and intensity, compactness ratio, and UHI. MODIS MOD11A2 V6 thermal band data from 2000, 2008, and 2016 were used to calculate land surface temperature (LST). The temperature typically varies from 24.50 C to 35.51 C during the day and 11.58 C to 22.61 C at night, according to the results. As demonstrated by the temperature ranges, the complete region has been divided into two classes: high temperature and low temperature. The output of land surface temperature shows that urban LST increases and the zone with high temperature have greatly extended.
Evaluation Method and Index System of Fault Diagnosis in Digitalized Distribution Substation Area
Authors: CHEN Jie , SHENG Wanxing , GUAN Shilei , ZHOU Feiyan , ZHAO Bo , SHI Changkai 
Published: January 2023

Abstract
Digitalized distribution substation area that constructed with intelligent transformer terminal could realize various advanced application scenarios by the use of IoT technology, which provides a new perspective for the fault diagnosis problem solving in power distribution system. This paper briefly introduces the principle of fault diagnosis based on digitalized substation area. Then propose a fault diagnosis effectiveness evaluation index system containing the first priority qualification index and the second priority performance index, and its index weight calculation method based on hierarchical analysis method and improved entropy weight method. And through the fuzzy comprehensive evaluation method, the comprehensive evaluation of the fault diagnosis effectiveness is carried out. At the end of this article, the effectiveness of the proposed evaluation method and index system is verified through example analysis.
Performance Analysis of Rate Adaptation Algorithms based on Mobility models and Propagation Loss in Wireless LANs
Authors: M Sri Vaishnavi , T Nishitha 
Published: January 2023

Abstract
Computer networks establish our day-to-day communications in this contemporary world. Wireless LANs (WLANs) have been beneficial for network connections within a small area referred to as a local area network. WLANs are elicited from the IEEE 802.11ac standard which is used in this paper. Because WLANs are widely used for networking purposes in order to augment the performance of wireless LANs rate adaptation algorithms are applied to the system. This paper primarily focuses on the performance of a few rate adaptation algorithms by changing different parameters’ average throughput and average delay so that their usage can be improvised in our daily lives.
Uncovering systems-level molecular similarities between Alzheimer’s and Parkinson’s diseases
Authors: Fallahi Hossein , Yadegari Zahra Sadat , Radak Mehran 
Published: January 2023

Abstract
Background: Two of the most prevalent central neuron system disorders are Alzheimer (AD) and Parkinson’s disease (PD). Interestingly, despite their differences in both pathological and molecular basis of the diseases, they exhibit some degrees of similarities. Here, we have conducted a comparative systems-level analysis study for these diseases. Cohort cortex samples from healthy control cases and AD/PD patients were obtained, then we have applied weighted gene co-expression network analysis (WGCNA).
Results: Network analysis identified key modules of genes related to each of these diseases. Gene ontology enrichment of the modules showed the involvement of both disease-specific and shared biological processes, including chemical synaptic transmission, nervous system development, and immune responses that are involved in both AD and PD. Surprisingly, the expression patterns for the gene members of the shared modules were strikingly identical. Additionally, we have introduced a handful of novel genes, including INPP4A, CREG2, ABI3, MYO1F, NAPB, NXN, DOCK6, CPSF6, and IKZF1, with potential functionality in both diseases; AD and PD.
Conclusions: In conclusion, besides unveiling the presence of high molecular level similarities between AD and PD, for the first time, several novel genes have been proposed that can open a new opportunity for diagnostic or treatment applications.
Combined adenovirus and Mycoplasma pneumoniae infection is an independent risk factor for developing severe community-acquired pneumonia in children
Authors: Lin Xiaoliang , Chen Qihong , Lan Yuanmei , Zhang Ning , Lin Lihua , Sun Qiufeng , Yang Yungang 
Published: January 2023

Abstract
Background: To investigate the pathogenic characteristics and risk factors of pediatric severe community-acquired pneumonia (CAP).
Methods:We retrospectively analyzed the clinical data of hospitalized children with severe CAP, including sex, age, results of sputum or bronchoalveolar lavage fluid (BALF) bacterial and fungal cultures, respiratory viruses, serum Mycoplasma pneumoniae (MP)-IgM and Chlamydia Pneumoniae (CP)-IgM, and BALF or blood (1-3)-β-D-glucan/galactomannan test.
Results:679 children with severe CAP were included in the analysis. The number of cases infected with MP was higher in males than in females. There were significant differences between the ≤1-year and >1-year groups, namely for bacterial, viral, MP, and CP infections. The top three bacteria cultured were Haemophilus influenza, Streptococcus pneumoniae, and Pseudomonas aeruginosa. The top three viruses detected were adenovirus (ADV), respiratory syncytial virus, and parainfluenza virus. The case numbers of ADV and MP infections in severe CAP were significantly higher than those of other infections, and ADV-positive infection was significantly associated with MP infection. MP infection was a risk factor for severe ADV-infected pneumonia, while sex, age, bacteria, CP, fungal, and ADV infections were risk factors for severe MP-infected pneumonia.
Conclusions: ADV-combined MP infection is an independent risk factor for the development of severe CAP in children.
Delayed rooting hormone application and stem cutting collection time improved Flacourtia indica rooting ability and root growth
Authors: Mng'omba Simon 
Published: January 2023

Abstract
Flacourtia indica is an important fruit and medicinal tree in southern Africa but has a low seed germination. Assessing rooting ability of mature (fruit bearing) F. indica stem cuttings is vital to attain early fruiting. The objectives were to assess responses of mature stem cuttings to different application time of a rooting hormone (Seradix® No. 2) and stem cutting collection time and to identify important pheno-phases of in F. indica yearly tree growth cycle. A significantly higher rooting percentage (P < 0.001) was obtained with delayed Seradix® No. 2 application than immediate application. Stem cuttings collected at flowering and leaf fall seasons were responsive to Seradix® No. 2 application, but all stem cuttings collected at fruiting time failed to produce roots. Stem cuttings significantly (P = 0.0482) produced more roots with immediate Seradix® No. 2 application than those subjected to delayed Seradix® No. 2 application for stem cuttings collected at flowering period. The failure to produce roots for cuttings collected at fruiting time could be due to low sugars (carbohydrates) in the stem cuttings. It is concluded that the best time to collect mature stem cuttings for rooting is either at flowering or leaf fall period and that delayed Seradix® No. 2 application improves rooting of mature F. indica stem cuttings.
Deep Handwritten Diagram Segmentation
Authors: Pravalpruk Buntita , Dailey Matthew 
Published: January 2023

Abstract
Handwriting has been a natural way to communicate since ancient times.Humans often prefer to write and draw to facilitate rapid exchange ofideas. When handwritten diagrams need to be converted to application-specific digital formats for later use, conversion requires skill and time.Automatic handwritten document conversion can save time, but dia-grams and text require different recognition engines. We propose tosolve the problem of segmentation of text and non-text elements ofhandwritten diagrams using deep semantic segmentation. Our model,DeepDP, can be tuned in complexity to a level appropriate for a particular dataset and diagram type. Experiments on a public flowchart datasetand a business process flow diagram dataset show excellent performance,with more than 95% accuracy, despite relatively small training datasets.
Evaluation of ocular surface involvement and systemic conditions in patients with autoimmune rheumatic diseases
Authors: Liu Yingyi , Wu Mengbo , Ren Yuerong , Feng Jianing , Shi Wen , Kang Huanmin , Tian Jing , He Yan 
Published: January 2023

Abstract
Purpose: To evaluate and explore the ocular surface involvement and systemic conditions in autoimmune rheumatic diseases (ARDs).
Methods: 79 patients with ARDs were enrolled in our study, including 26 patients with rheumatoid arthritis (RA), 33 patients with systemic lupus erythematosus (SLE), and 20 patients with primary Sjögren’s syndrome (pSS). All patients underwent ocular surface evaluation, including ocular surface symptoms and signs, conjunctival impression cytology, and tear multi-cytokine detection. The systemic conditions were also collected, including disease duration and disease activity.
Results: SLE patients have the shortest disease duration and nearly half of them have low disease activity, while RA patients and pSS patients have a relatively long disease duration and about 90% of them have moderate/high disease activity. The incidence of dry eye and the levels of pro-inflammatory tear cytokine in SLE patients is significantly lower than RA and pSS patients, while there was no significant difference between RA and pSS patients. However, pathologic squamous metaplasia on the ocular surface is more severe in SLE and pSS patients than RA patients. Dry eye severity in all ARDs patients was shown independent of disease activity, while the ocular surface Nelson’s grades are positively correlated with disease duration in RA patients.
Conclusions: Dry eye and ocular surface inflammation persist in most ARDs patients, and do not occur in parallel with the disease activity. Other than pSS, dry eye and ocular surface squamous metaplasia also exist in SLE and RA. Therefore, all patients with ARDs require a regular ophthalmologic evaluation and topical medications.
Screening of several microalgae revealed biopesticide properties of Chlorella sorokiniana against the strawberry pathogen Phytophthora cactorum
Authors: Jokel Martina , Salazar João , Chovancek Erik , Sirin Sema , Allahverdiyeva Yagut 
Published: January 2023

AbstractPresent agricultural production typically depends on the intensive use of synthetic pesticides with potentially harmful consequences for humans and the environment. To ensure food security for the rapidly rising global population it is necessary to develop more sustainable alternatives to synthetic pesticides. Microalgae possess a large diversity in antimicrobial compounds and are considered one of the most promising sustainable sources of novel biopesticides. Antimicrobial activities of 15 microalgae strains were investigated against a selection of seven common plant pathogens relevant to agricultural production. Several microalgae were identified to possess antimicrobial activity with an extract ofChlorella sorokinianashowing the strongest growth inhibition of the plant pathogenPhytophthora cactorum. Different pre-treatments like freeze-drying, solvents with different polarities, and extraction methods were analyzed in regards to the level of antimicrobial activity ofC. sorokiniana. The bestC. sorokinianaextract demonstrated potential for biopesticide application on strawberry leaves infected withP. cactorum. This study reveals the abundant potential of microalgae as natural biopesticide for organic or more sustainable regular agriculture.