bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disopyramide Revisited for Treatment of Symptomatic Obstructive Hypertrophic Cardiomyopathy: Efficacy and Safety in Patients Treated for at Least 5 Years
Authors: Massera Daniele , Sherrid Mark V. , Adlestein Elizabeth , Bokhari Nadia , Alvarez Isabel C. , Wu Woon Y. , Reuter Maria C. , Maron Martin S. , Maron Barry J. , Rowin Ethan J. 
Published: January 2025

Background
Disopyramide is used to treat heart failure symptoms in patients with obstructive hypertrophic cardiomyopathy (HCM) with known medium‐term efficacy and safety, while long‐term outcomes are unknown.

Methods and Results

A total of 92 consecutive patients with symptomatic obstructive HCM with peak left ventricular outflow tract gradients of ≥30 mm Hg at rest or with provocation who were maintained on disopyramide for ≥5 years at 2 dedicated HCM centers were included: 92 patients; mean age, 62.5 years; 54% women; treated with disopyramide for median 7.2 years (left ventricular wall thickness 18±4 mm; median peak outflow gradient 95 mm Hg). At last follow‐up, 62 (67%) patients continued disopyramide, including 57 with symptom improvement ≥1 New York Heart Association class. The other 30 (33%) patients discontinued disopyramide due primarily to incomplete symptom resolution and required surgical myectomy or alcohol septal ablation (n=23) at 7.4 years from initiation. With disopyramide, resting left ventricular outflow gradients were reduced by 37% (to median 19 mm Hg), and provoked gradients decreased by 57% (to median 41 mm Hg), with no residual outflow obstruction (<30 mm Hg at rest or with provocation) in 42 (46%) patients and no change in ejection fraction (69%±6% versus 69%±9%,
P
=0.51). Ventricular tachyarrhythmias and left ventricular systolic dysfunction were uncommon (n=3 and n=1) and were not attributed to disopyramide. Death on disopyramide was exceedingly rare (n=3 [5%]) and non–HCM‐related occurring at age ≥90 years.

Conclusions
In patients with obstructive HCM, disopyramide is safe and effective at relieving heart failure symptoms from outflow obstruction in a subgroup of patients who were maintained on disopyramide for >5 years.
Assessing the Likelihood of Procedural Intubation During Percutaneous Coronary Intervention
Authors: Herson Megan , Tan Sean , O'Brien Joseph , Teoh Zhi Hao , Manmathan Gavin P. R. , Dinh Diem , Brennan Angela , Brown Adam J. , Chew Derek P. 
Published: January 2025

Background
Preprocedural fasting is widely used before percutaneous coronary intervention (PCI). However, the incidence of procedural intubation during PCI is unknown. This study aims to identify the incidence and predictors for procedural intubation during PCI.

Methods and Results

A retrospective cohort study was performed on patients undergoing PCI between 2014 and 2021 within the Victorian Cardiac Outcomes Registry. Patients were classified into urgent, semiurgent, or elective PCI on the basis of recorded PCI indication. Those undergoing semiurgent or elective PCI were presumed to be fasted preprocedurally. The incidence of procedural intubation was reported for each PCI group. Inverse probability treatment weighting was used to determine the association between 30‐day death and procedural intubation. Logistic regression was performed to determine clinical, procedural, and lesion characteristics associated with procedural intubation among fasted patients. Among 83 929 patients, the incidence of procedural intubation was 0.5%. Patients undergoing urgent PCI (odds ratio [OR], 19.68 [95% CI, 13.91–27.86];
P
<0.01) had the highest risk of procedural intubation. Procedural intubation was associated with increased 30‐day death (OR, 4.79 [95% CI, 3.29–6.96];
P
<0.01). Among fasted patients, estimated glomerular filtration rate (OR, 0.99 [95% CI, 0.98–0.99];
P
<0.01), cardiogenic shock (OR, 96.24 [95% CI, 56.01–165.35];
P
<0.01), out‐of‐hospital cardiac arrest (OR, 3.42 [95% CI, 1.30–8.97];
P
=0.01), femoral access (OR, 2.09 [95% CI, 1.38–3.19];
P
<0.01), left main disease (OR, 5.77 [95% CI, 3.16–10.54];
P
<0.01), type C lesions (OR, 3.86 [95% CI, 1.19–12.56];
P
=0.03), and rotational atherectomy (OR, 2.20 [95% CI, 1.03–4.69];
P
=0.04) were associated with procedural intubation.

Conclusions
Despite an association with a worse 30‐day mortality rate, the incidence of procedural intubation during PCI was low. Universal preprocedural fasting may be unnecessary and could be targeted at higher‐risk groups.
Number of Previous Strokes and the Association With Clinical Outcomes of Patients With Atrial Fibrillation: Longitudinal Data From the GLORIA‐AF Registry
Authors: Lam Steven Ho Man , Romiti Giulio Francesco , Corica Bernadette , Bucci Tommaso , Olshansky Brian , Chao Tze‐Fan , Huisman Menno V. , Lip Gregory Y. H. ,   the GLORIA‐AF Investigators
Published: January 2025

Background
Patients with atrial fibrillation (AF) who suffered a previous stroke are at increased risk of recurrent thromboembolic events and other major outcomes. The impact of the number of stroke episodes on the natural history of patients with AF is still unclear.

Methods and Results
Using data from the international, multicenter, and prospective GLORIA‐AF (Global Registry on Long‐Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation) Registry Phase III, we categorized patients with a recent diagnosis of non‐valvular AF according to the number of previous strokes (either 0, 1, or ≥2 episodes). We analyzed use of oral anticoagulants through multiple logistic regression model, and risk of major outcomes using multiple Cox‐regression models; our primary outcome was all‐cause death. Among 21 223 patients (mean age: 70.2±10.3 years; 44.9% female) included, 2251 (10.6%) had a previous history of stroke, and 216 (1.0%) had ≥2 or more strokes. Oral anticoagulants were used in ≥80% of patients regardless of the numbers of previous stroke, although those with 1 (versus >1) prior stroke showed lower odds of receiving oral anticoagulants (odds ratio [95% CI]: 0.83 [0.73–0.94]). During 3‐years follow‐up, the risk of all‐cause mortality increased with the number of previous strokes (hazard ratio [95% CI]: 1.46 [1.28–1.67] and 2.43 [1.79–3.29] for 1 versus 0 and ≥2 versus 0 previous strokes, respectively). Similar results were observed for other secondary outcomes, including thromboembolism, but not for major bleeding.

Conclusions
History of stroke still represents a key risk factor in patients with AF. Patients who suffered more than 1 episodes of stroke had significantly worse prognosis and further efforts may be required to improve their clinical outcomes.
Nootkatone inhibits the progression of glioblastoma by activating the ATF4-CHOP-CHAC1 pathway
Authors: Wang Qian , Xue Xiumin , Chen Zhichao , Zhang Wei , Qian Yiming , Chen Danni , Lin Lin , Yuan Yinfeng , Zhao Weiqiao , Huang Zhihui , Wang Yongjie 
Published: January 2025

Abstract
Glioblastoma multiforme (GBM) represents a primary brain tumor that is widely prevalent, and clinical drugs available for its treatment exhibit varying degrees of resistance. Nootkatone (NKT) is a functional sesquiterpene sourced from traditional Chinese medicine --Alpinia Oxyphylla Miq and has been reported to have a diverse range of pharmacological properties. However, it remains unknown whether there are effects of NKT on GBM. In this study, we found that NKT inhibited the growth of GBM cells in a dose-dependent manner in vitro. Subsequently, we observed that NKT suppressed the migration and arrested cell cycle at G2/M phase of GBM cells. Furthermore, NKT induced the death of GBM cells accompanied by an increase in reactive oxygen species (ROS) production. Mechanistically, we found that NKT inhibited the progression of GBM cells through activating the ATF4-CHOP-CHAC1 pathway in GBM cells. Furthermore, NKT-induced inhibition of migration and proliferation in GBM cells was partially restored by ATF4 or CHAC1 knockdown. Finally, we found that NKT inhibited the growth of tumor in GBM orthotopic mice model through activation of ATF4-CHOP-CHAC1 axis. Taken together, our findings show that NKT suppresses the growth and migration of GBM cells by activating the ATF4-CHOP-CHAC1 pathway, which in turn prevents the tumorigenesis of GBMs and provides a novel perspective for the development of drugs against GBM.

Graphical Abstract
Brain computer interfaces for cognitive enhancement in older people - challenges and applications: a systematic review
Authors: Tsai Ping-Chen , Akpan Asangaedem , Tang Kea-Tiong , Lakany Heba 
Published: January 2025

Abstract

Background
Brain-computer interface (BCI) offers promising solutions to cognitive enhancement in older people. Despite the clear progress received, there is limited evidence of BCI implementation for rehabilitation. This systematic review addresses BCI applications and challenges in the standard practice of EEG-based neurofeedback (NF) training in healthy older people or older people with mild cognitive impairment (MCI).

Methods
Articles were searched via MEDLINE, PubMed, SCOPUS, SpringerLink, and Web of Science. 16 studies between 1st January 2010 to 1st November 2024 are included after screening using PRISMA. The risk of bias, system design, and neurofeedback protocols are reviewed.

Results
The successful BCI applications in NF trials in older people were biased by the randomisation process and outcome measurement. Although the studies demonstrate promising results in effectiveness of research-grade BCI for cognitive enhancement in older people, it is premature to make definitive claims about widespread BCI usability and applicability.

Significance
This review highlights the common issues in the field of EEG-based BCI for older people. Future BCI research could focus on trial design and BCI performance gaps between the old and the young to develop a robust BCI system that compensates for age-related declines in cognitive and motor functions.
Do orthodontists consider endodontic complications in their orthodontic management of teeth with a history of dental trauma? A vignette survey
Authors: Awad Kinda , Hourani Ammar Al , Kasperek Dariusz , Jarad Fadi , Albadri Sondos 
Published: January 2025

Abstract

Background
Dental trauma is a frequent injury seen commonly in young children. There is a link between malocclusion and the incidence of traumatic dental injuries. Orthodontic therapy has been suggested as a preventative measure in correcting unfavourable malocclusions and potentially avoiding traumatic dental injuries. To date, it is poorly understood if the complications reported following traumatic dental injuries are amplified during and following orthodontic treatment. The aim of this study was to evaluate whether orthodontists considered the endodontic implications associated with the orthodontic treatment of teeth with a history of dental trauma.

Method
A mixed methods vignette survey was designed, piloted, and distributed online to UK registered specialist orthodontists. The survey was split into three parts and consisted of three vignette clinical scenarios with open and closed questions.

Results
A total of 76 orthodontists responded from the United Kingdom. Of the participants, 46% (n = 35) of the orthodontists felt they had insufficient training in dental trauma and 42% (n = 32) lacked confidence in the treatment of traumatic injuries. The study participants reported non-standardised pre- treatment examination, limited dental trauma experience and insufficient training. In addition, 32% (n = 24) of clinicians felt that there is a lack of guidance in the orthodontic management of traumatised teeth and pulpal sequelae.

Conclusion
Orthodontists are not following a standardised protocol in their examination of teeth with a history of trauma prior to orthodontic treatment. There is a need within the orthodontic specialty to create a standardised protocol to assess teeth with a history of dental trauma.
Empowering personalized oncology: evolution of digital support and visualization tools for molecular tumor boards
Authors: Strantz Cosima , Böhm Dominik , Ganslandt Thomas , Börries Melanie , Metzger Patrick , Pauli Thomas , Blaumeiser Andreas , Scheiter Alexander , Jung Ian-Christopher , Christoph Jan , Manuilova Iryna , Strauch Konstantin , Ustjanzew Arsenij , Reimer Niklas , Busch Hauke , Unberath Philipp 
Published: January 2025

Abstract

Background
Molecular tumor boards (MTBs) play a pivotal role in personalized oncology, leveraging complex data sets to tailor therapy for cancer patients. The integration of digital support and visualization tools is essential in this rapidly evolving field facing fast-growing data and changing clinical processes. This study addresses the gap in understanding the evolution of software and visualization needs within MTBs and evaluates the current state of digital support. Alignment between user requirements and software development is crucial to avoid waste of resources and maintain trust.

Methods
In two consecutive nationwide medical informatics projects in Germany, surveys and expert interviews were conducted as stage 1 (n = 14), stage 2 (n = 30), and stage 3 (n = 9). Surveys, via the SoSci Survey tool, covered participants' roles, working methods, and support needs. The second survey additionally addressed requirements for visualization solutions in molecular tumor boards. These aimed to understand diverse requirements for preparation, implementation, and documentation. Nine semi-structured expert interviews complemented quantitative findings through open discussion.

Results
Using quantitative and qualitative analyses, we show that existing digital tools may improve therapy recommendations and streamline MTB case preparation, while continuous training and system improvements are needed.

Conclusions
Our study contributes to the field by highlighting the importance of developing user-centric, customizable software solutions that can adapt to the fast-paced environment of MTBs to advance personalized oncology. In doing so, it lays the foundation for further advances in personalized medicine in oncology and points to a shift towards more efficient, technology-driven clinical decision-making processes. This research not only enriches our understanding of the integration of digital tools into MTBs, but also signals a broader shift towards technological innovation in healthcare.
Solidarity and its decoloniality in global health ethics
Authors: Fayemi Ademola Kazeem , Kirchhoffer David Gerrard , Pratt Bridget 
Published: January 2025

Abstract
Solidarity is one of the emerging values in global health ethics, and a few pieces of bioethics literature link it to decoloniality. However, conceptions of solidarity in global health ethics are influenced primarily by Western perspectives, thus suggesting the decolonial needs to include non-Western perspectives. This article explores a decolonial interpretation of solidarity to enrich our understanding of solidarity. It employs a palaver approach, typical of African (Yorùbá) relational culture, in developing a conception of solidarity grounded in a beehive metaphor. Through a decolonial methodological approach, this article posits that a beehive metaphor allegorically symbolises solidarity. In this decolonial interpretive account, solidarity embeds relational virtues and duties that foster harmony. Solidarity is a positively oriented affective disposition with people with whom one shares similar circumstances for harmonious well-being through concerted efforts. This article addresses five potential objections to this account of solidarity in global health ethics and consequently explores what an African account of solidarity means for global health research funding. This article concludes that the palaver decolonial approach from the Global South has implications for expanding conceptual perspectives on solidarity in global health ethics.
Exploring the power of spectrophotometric technique in determination of oxytetracycline and lidocaine in their pharmaceutical dosage form as well as in the presence of toxic lidocaine impurity: univariate versus multivariate analysis
Authors: Ahmed Naglaa , Hemdan Ahmed , ZaaZaa Hala , Galal Maha 
Published: January 2025

Abstract
Lidocaine poses challenges when it comes to direct spectrophotometric measurement due to the lack of sharp peak within its spectra in zero-order. This lack of a distinct peak makes it difficult to accurately quantify lidocaine using traditional direct spectrophotometric methods. In our study, different univariate and multivariate spectrophotometric techniques have been established and their validity has been assessed for the determination of the mixture of Lidocaine HCl (LD), Oxytetracycline HCl (OTC) together with LD carcinogenic impurity [2,6- dimethylaniline] DMA. LD was resolved from the other two components using ratio difference and derivative ratio methods. OTC was determined in zero- order at 360 nm and by using constant value and concentration value methods, while DMA was determined by using constant multiplication at 237 nm as well as by using constant value and concentration value methods after elimination of OTC by ratio subtraction technique. Moreover, Partial Least Squares and Principal Component Regression multivariate approaches were applied to quantify and evaluate the mixture. The developed methods underwent validation following International Council for Harmonization guidelines. The validation process demonstrated that all suggested methods are accurate and selective in their measurements. Additionally, statistical analysis was conducted to compare the developed and reported methods. Furthermore, one-way analysis of variance was performed to compare both proposed and reported spectrophotometric methods.
Identification of Southeast Asian Anopheles mosquito species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a cross-correlation approach
Authors: Chaumeau Victor , Sawasdichai Sunisa , Min Thu Zar Ma Ma Moe , Kularbkeeree Thithiwarada , Jaruwan Naw , Gloria Naw , Lee Naw Yu , Trackoolchengkaew Muesuwa , Phanaphadungtham Monticha , Rongthong Patcharamai , Inta Aritsara , Watthanaworawit Wanitda , Nosten François 
Published: January 2025

Abstract

Background
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) is proposed for mosquito species identification. The absence of public repositories sharing mass spectra and open-source data analysis pipelines for fingerprint matching to mosquito species limits the widespread use of this technology. The objective of this study was to develop a free open-source data analysis pipeline for Anopheles species identification with MALDI–TOF MS.

Methods

Anopheles mosquitoes were captured in 33 villages in Karen (Kayin) state in Myanmar. A subset of 403 specimens was selected for inclusion in either the reference or the test panel (270 and 133 specimens, respectively). Three hundred fifty-nine specimens could be identified with DNA barcodes and were assigned to 21 sensu stricto species and five sibling species pairs or complexes. A total of 3584 mass spectra of the head of these specimens identified with DNA barcoding were acquired and the similarity between mass spectra was quantified using a cross-correlation approach adapted from the published literature. A reference mass spectra database was created using all spectra of the PCR-identified specimens assigned to the reference panel. A simulation experiment was carried out by querying the reference database with the spectra of the test panel to evaluate the performance of species identification with MALDI–TOF MS at varying thresholds of the cross-correlation index for the algorithm to output an identification result and with varying numbers of technical replicates for the tested specimens, considering PCR identification results as the reference.

Results
With one spot and a threshold value of −14 for the cross-correlation index on the log scale, the sensitivity was 0.99 [95% credible interval (CrI): 0.98–1.00], the predictive positive value was 0.99 (95% CrI: 0.98–0.99), and the accuracy was 0.98 (95% CrI: 0.97–0.99). It was not possible to directly estimate the sensitivity and negative predictive value because there was no true negative (i.e., queries of species not referenced in the database) in the assessment.

Conclusions
The cross-correlation approach can be used to match mass spectral fingerprints to predefined taxa. MALDI–TOF MS is a valuable tool for rapid, accurate, and affordable identification of Anopheles species.

Graphical abstract
Reviewing manuscripts for scientific journals: recommendations for early career scientists
Authors: Forero Diego A. , Glatt Stephen J. , Oermann Marilyn H. 
Published: January 2025

Abstract
Publication of articles in international scientific journals has been one of the main strategies for the communication of scientific findings and ideas. Prepublication peer review is a fundamental aspect of the publishing process in indexed scientific journals and, associated with the large growth in journals and articles, there has been a recent challenge in having adequate peer reviewers for international journals. In this article, we provide a short overview of the publishing process, give recommendations to early career researchers about writing peer reviews of adequate quality, and discuss some possibilities for the future.
TRIM8 inhibits porcine epidemic diarrhoea virus replication by targeting and ubiquitinately degrading the nucleocapsid protein
Authors: Bi Zhenbin , Wang Wei , Gu Shanshen , Zhou Yajing , Wu Zhengchang , Bao Wenbin , Wang Haifei 
Published: January 2025

Abstract
Porcine epidemic diarrhoea virus (PEDV) is an enteric pathogen that causes acute diarrhoea, dehydration and high mortality rates in suckling pigs. Tripartite motif 8 (TRIM8) has been shown to play multiple roles in the host’s defence against viral infections. However, the functions of TRIM8 in regulating PEDV infection are still not well understood. In our study, we found a significant upregulation of TRIM8 following PEDV infection. We created TRIM8 knockout and overexpression cell lines and discovered that TRIM8 can inhibit PEDV replication within host cells. Co-immunoprecipitation assays revealed that TRIM8 directly interacts with the nucleocapsid protein (N) of PEDV, specifically within the coiled-coil structural domain of TRIM8. Furthermore, TRIM8 was shown to reduce the expression of the PEDV N protein in a dose-dependent manner. Mechanistically, TRIM8 inhibits the expression of PEDV N through K48-linked ubiquitin proteasome degradation. Transcriptomics analysis revealed that TRIM8 facilitates the expression of genes associated with several pathways, including the IL-17 signalling pathway, chemokine signalling pathway, and cytokine-cytokine receptor interaction. This suggests that TRIM8 plays a crucial role in boosting antiviral immune responses against PEDV infection. Our findings provide new insights into the functions and mechanisms of TRIM8 in regulating PEDV infection and highlight its potential as a molecular target for the prevention and control of this virus.