bluuebunny/crossref_metadata_2025_split huggingface dataset:
ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy
Authors: Sánchez-Martín Pablo , Kriegenburg Franziska , Alves Ludovico , Adam Julius , Elsaesser Jana , Babic Riccardo , Mancilla Hector , Licheva Mariya , Tascher Georg , Münch Christian , Eimer Stefan , Kraft Claudine 
Published: January 2023

ABSTRACT
Autophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double-membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment is a tightly regulated step and involves membrane-bound SNARE proteins. These play a crucial role as they promote lipid mixing and fusion of the opposing membranes. Among the SNARE proteins implicated in autophagy, the essential SNARE protein YKT6 is the only SNARE protein that is evolutionarily conserved from yeast to humans. Here, we show that alterations in YKT6 function, in both mammalian cells and nematodes, produce early and late autophagy defects that result in reduced survival. Moreover, mammalian autophagosomal YKT6 is phospho-regulated by the ULK1 kinase, preventing premature bundling with the lysosomal SNARE proteins and thereby inhibiting autophagosome–lysosome fusion. Together, our findings reveal that timely regulation of the YKT6 phosphorylation status is crucial throughout autophagy progression and cell survival.
Fe, N-Doped SrTiO3 Synthesized Using Pyrazine Carboxylic Acid-Metal Complexes: Application as an Oxygen Reduction Catalyst for Polymer Electrolyte Fuel Cell Cathodes in Acidic Media
Authors: Takeuchi Yuu , Matsuzawa Koichi , Nagai Takaaki , Ikegami Kaoru , Kuroda Yoshiyuki , Monden Ryuji , Ishihara Akimitsu 
Published: January 2023

Abstract
Fe, N-doped SrTiO3 was successfully synthesized using pyrazine carboxylic acid-metal complexes as the starting materials and exhibited high onset potential of 0.8 V vs. RHE for the oxygen reduction reaction because of the formation of high-quality active sites by Fe and N doping, and electron conduction paths by carbon nanotubes.
Impact of clinical factors on hospital stay and hospital readmission rate after acute exacerbation of COPD: a retrospective cross-sectional study
Authors: Chaurasiya Prem Shankar , Lamsal Dinesh Kumar , Khatri Akash , Aryal Laxmi 
Published: January 2023

Background: Chronic obstructive pulmonary disease (COPD) is a condition with high morbidity and cost to health services due to a high number of exacerbations necessitating multiple and prolonged hospitalizations. The length of hospital stays and hospital readmission rate is related to patient age, sex, disease severity, current smoking status, comorbidities, dyspnea grade, carbon dioxide partial pressures, use of mechanical ventilation, previous exacerbation, long-term oxygen therapy, and inpatient diuretics use. The purpose of the study was to identify the differential effects of patient and treatment-related factors on the length of hospital stay and hospital readmission for COPD exacerbation-related admissions. Methods: A hospital-based retrospective cross-sectional study was conducted among 151 patients with acute exacerbation of COPD admitted at Civil Service Hospital, Kathmandu from August 2021 to March 2022. Ethical approval was taken from the Institutional Review Committee, Civil Service Hospital (Reference no. 18/ 2022). A convenience sampling method was followed. Data regarding different clinical factors were collected in a semi-structured questionnaire. The data were entered and analyzed through Microsoft Excel 365 and SPSS version 22.0 using a binary regression model. Results: Comorbidities, current smoking, dyspnea grade mMRC IV, mechanical ventilation, and long-term oxygen therapy were significantly associated with prolonged hospital stays for COPD exacerbation-related admissions. The corresponding odd ratio is (OR 3.4, 95% CI: 1.24–9.29); (OR 21.4, 95% CI: 6.17–74.57); (OR 2.5, 95% CI: 1.20–5.45); (OR 5.6, 95% CI: 1.20–26.35); (OR 2.4, 95% CI: 1.02–5.90), respectively. Conclusions: The effect of clinical factors such as comorbidities status, current smoking habits, higher grade of mMRC dyspnea scale, mechanical ventilation, and long-term oxygen therapy needed to be considered to optimize care for COPD patients needing hospital admissions and hence decrement in hospital costs.
Second-line drug resistance markers as proxy indicators of time to sputum culture conversion among second-line drug resistant tuberculosis patients tested in Uganda: A cross-sectional study
Authors: Mujuni Dennis , Ssengooba Willy , Ibanda Ivan , Kabugo Joel Solomon , Kasemire Dianah Linda , Nampewo Elizabeth , Nsawotebba Andrew , Phelan Jody E , Tugumisirize Didas , Orena Beatrice , Byabajungu Henry , Ntenkaire Nathan , Nadunga Diana , Tumwine Julius , Musisi Kenneth , Joloba Moses , Kim Seungmo , Kokas Ikwap , Mukani William Olaho , Kungu Joseph , Afayoa Mathias 
Published: January 2023

Background Increased tuberculosis disease burden arises as a result of low treatment success rates stemming from the emergence of second-line drug resistance. We aimed at determining the usefulness of second-line drug (SLD) resistance markers as proxy indicators of time to sputum culture conversion; a renowned predictor of Tuberculosis treatment outcome, among SLD-resistant tuberculosis (TB) patients tested at the Uganda National TB Reference Laboratory (NTRL). Methods A cross-sectional study was conducted on 72 bacteriologically confirmed SLD resistant TB patients with datasets including culture conversion time and second line probe assay mutation profiles between 01/06/2017 and 31/12/2019. The data were then imported into STATA v15 for descriptive statistical analysis, Univariate cox proportional hazard model analysis and Kaplan-Meier survival curves at a 5% level of significance; p-value ≤0.05. Results Results indicate the median time was achieved at 3 (0–12) months across the studied patients. The rrs G1484T mutation associated with conferring drug resistance to injectable agents was observed to have the shortest median conversion time of 1.5 months, longest by the gryB E540D at 5 months. A single mutation in the gryA gene locus showed higher converted proportions 70.8% (58.9–81.0) than those that had two 8.3% (3.1–17.3) or three 2.7% (0.3–10.0) mutations. Conclusions The studied second-line drug resistance markers had no statistically significant association with the time to sputum culture conversion, although increased drug resistance levels reduced the converted proportions and stressed the need to utilize molecular diagnostics data and other crucial variables to better comprehend proxy indicators of SLD resistant tuberculosis management.
Comparación de anticuerpos postvacunales al SARS-CoV-2 en casos asintomáticos, casos sintomáticos, y no casos de una cohorte de trabajadores de un hospital general
Authors: Sabater Vidal Susana , Tirado-Balaguer María Dolores , Soria-Martín Raquel , Arnedo-Pena Alberto , Gil-Galdón Abel , Moreno-Muñoz Rosario , Bellido-Cambrón Mª Carmen 
Published: January 2023

Estimada Sra. Directora:
Agradecemos a los doctores Rujittika Mungmunpuntipantip y Viroj Wiwanitkit su interés por nuestra publicación(1) en la carta en la que se subrayan la importancia de los casos asintomáticos de COVID-19 en la evaluación de los efectos de la vacunación anti-SARS-CoV-2(2). Compartimos con ellos que las personas asintomáticas al COVID-19 pueden presentar unos niveles de anticuerpos anti-SARS-CoV-2 IgG-S mayores que las personas sin historia previa de COVID-19, y que pueden diferir en las reacciones a la vacunación, considerando que la prevalencia de COVID-19 asintomáticos se ha descrito como elevada(3).
En nuestra cohorte de trabajadores del Hospital General Universitario de Castellón, se detectaron 5 casos de COVID-19 asintomáticos (CA), incluyendo los dos seguimientos realizados(1,4), y 20 casos presentaron síntomas de COVID-19 (CS), con un total de 25 casos con confirmación por el laboratorio, 20 % tasa de asintomáticos (5/25). En la tabla 1 se recogen las características de los CA, CS, y de los participantes que no habían sufrido la enfermedad. Los CA eran más jóvenes que los otros 2 grupos, y la proporción de varones era significativamente mayor (p=0,027). En cuanto a los anticuerpos Anti-SARS-CoV-2 IgG-S al mes de la vacunación, los niveles de los CS fueron mayores que los de CA, y de los no casos, siendo estos últimos los que tuvieron significativamente menores niveles (p<0,001). Sin embargo, a los 8 meses de la vacunación la caída de IgG-S fue general, y los niveles de IgG-S eran mayores en los CA que en los CS y en los no casos (p<0,001). Los niveles de IgG-S considerados como protectores ? 4160 UA/ml, eran mayores en los CA y CS que el de los no casos (p=0,001). Si bien, el declive era similar en los tres grupos (p=0,084). Los síntomas y los efectos secundarios de las dos dosis de vacuna Pfizer-BioNTech no presentaron diferencias significativas entre los grupos.
Estos resultados son coincidentes con númerosos estudios, en los que se constata que los casos de COVID-19 presentan niveles más elevados de IgG-S que las personas que no han sufrido la enfermedad(5), y se apreció que en valores medio no se alcanzaron los niveles de IgG-S protectores. De aquí la importancia de disponer de marcadores más efectivos de la situación de protección de la personas vacunadas tanto si han sufrido la enfermedad como sino. Además de los anticuerpos neutralizantes, la determinación de la inmunidad celular podría ser muy conveniente para conocer los niveles de protección.
CircStrn3 targeting microRNA-9-5p is involved in the regulation of cartilage degeneration and subchondral bone remodelling in osteoarthritis
Authors: Li Bin , Ding Tao , Chen Haoyi , Li Changwei , Chen Bo , Xu Xing , Huang Ping , Hu Fangqiong , Guo Lei 
Published: January 2023

Aims Circular RNA (circRNA) is involved in the regulation of articular cartilage degeneration induced by inflammatory factors or oxidative stress. In a previous study, we found that the expression of circStrn3 was significantly reduced in chondrocytes of osteoarthritis (OA) patients and OA mice. Therefore, the aim of this paper was to explore the role and mechanism of circStrn3 in osteoarthritis. Methods Minus RNA sequencing, fluorescence in situ hybridization, and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expression of circStrn3 in human and mouse OA cartilage tissues and chondrocytes. Chondrocytes were then stimulated to secrete exosomal miR-9-5p by cyclic tensile strain. Intra-articular injection of exosomal miR-9-5p into the model induced by destabilized medial meniscus (DMM) surgery was conducted to alleviate OA progression. Results Tensile strain could decrease the expression of circStrn3 in chondrocytes. CircStrn3 expression was significantly decreased in human and mouse OA cartilage tissues and chondrocytes. CircStrn3 could inhibit matrix metabolism of chondrocytes through competitively ‘sponging’ miRNA-9-5p targeting Kruppel-like factor 5 ( KLF5), indicating that the decrease in circStrn3 might be a protective factor in mechanical instability-induced OA. The tensile strain stimulated chondrocytes to secrete exosomal miR-9-5p. Exosomes with high miR-9-5p expression from chondrocytes could inhibit osteoblast differentiation by targeting KLF5. Intra-articular injection of exosomal miR-9-5p alleviated the progression of OA induced by destabilized medial meniscus surgery in mice. Conclusion Taken together, these results demonstrate that reduction of circStrn3 causes an increase in miR-9-5p, which acts as a protective factor in mechanical instability-induced OA, and provides a novel mechanism of communication among joint components and a potential application for the treatment of OA. Cite this article: Bone Joint Res 2023;12(1):33–45.
Influence of Linguistic Ideology on the Concept of Codification of the First Ancient Chinese Dictionaries
Authors: Kostanda Iryna 
Published: January 2023

Abstract: One of the important directions of research in modern linguistics is the analysis of the processes of language standardization, as well as linguistic and extralinguistic factors on which the direction of the vector of these processes depends. Based on the model of linguistic planning proposed by Einar Haugen, the codification process is defined as the second step, which is preceded by the process of language material selection. However, the success of implementation of the codified phenomena depends on the form of their implementation, as well as on the processes of selection of the language material. It is the processes of selection and the form of introduction of codification that remain insufficiently covered in the studies of Ancient China language planning processes. Most research works in this area are not devoted to the analysis of the criteria for selecting the form and the concept of codification, but to the origin of ancient Chinese dictionaries and their means of decoding. Thus, the first carriers of the codification of Ancient China are the dictionaries “Erya”, “Fangyan”, “Shiming”, “Shuowen”. Taking these dictionaries as examples, we can see that the forms of codification have a great variability.
Design and Implementation of Intelligent Vegetable Recognition System based on MobileNet
Authors: Yang Linlin , Song Yingluo , Hu Zilin , Wang Aili , Wu Haibin , Iwahori Yuji 
Published: January 2023

With the rise of food safety traceability, unmanned supermarkets and autonomous shopping, the automatic identification technology of agricultural products such as vegetables in circulation and sales has become an urgent problem. This paper designs an intelligent vegetable identification system based on MobileNet to solve intelligent identification problem of vegetable sales in supermarkets.
The system includes main control core, visual processing module, pressure sensor, voice broadcasting module and display module. When the system detects that there are vegetables to be weighed, the visual processing module completes the classification of vegetables, broadcasts the name, unit price and total price of vegetables by voice, and displays the weight, unit price and total price by OLED. The machine vision processing module is constructed by deep separable convolution (DSC). It realizes the separation of channels and regions, so it has high computing efficiency and is more suitable for embedded devices with low memory space.
The experimental results show that the overall recognition rate of five vegetables reaches 97.33% under three kinds of illumination. The system has the advantages of stability, intelligence and convenience.
Faktor-Faktor yang Berhubungan dengan Kejadian Hipertensi di Desa Baet Lampuot Aceh Besar
Authors: Candra Aditya , Santi Tahara Dilla , Yani M. , Mawaddah Devy Surya 
Published: January 2023

Latar Belakang: Hipertensi adalah kelainan sistem sirkulasi darah yang mengakibatkan peningkatan tekanan darah diatas nilai normal atau tekanan darah ≥140/90 mmhg. Faktor resiko hipertensi dibagi menjadi 2 kelompok yaitu yang dapat dirubah seperti merokok, konsumsi garam berlebih, obesitas, kurang aktivitas fisik, dan stress dan tidak dapat diubah (usia, jenis kelamin, dan genetik). Hipertensi dapat menimbulkan beberapa komplikasi seperti penyakit jantung, gagal ginjal, diabetes dan stroke. Penanganan hipertensi meliputi farmakologi dengan obat antihipertensi dan non farmakologi dengan menjalani gaya hidup sehat. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang berhubungan dengan kejadian hipertensi pada masyarakat di desa Baet Lampuot.Metode: Penelitian kuantitatif dengan pendekatan cross sectional, teknik sampel menggunakan non-probability sampling dengan teknik accidental sampling. Analisa data menggunakan distribusi frekuensi dan chi square analisis.Hasil: Berdasarkan hasil penelitian terdapat hubungan antara umur responden dengan risiko terjadinya hipertensi dengan p value 0,026 dan terdapat hubungan yang signifikan antara jenis kelamin responden dengan risiko terjadinya hipertensi dengan p value 0,024.Kesimpulan: Terdapat hubungan yang signifikan antara usia dan jenis kelamin terhadap kejadian hipertensi di desa Baet Lampuot.Kata Kunci : hipertensi; usia; jenis kelamin ABSTRACTTitle: Factors Associated with the Incidence of Hypertension in Baet Lampuot Village Aceh BesarBackground: Hypertension is a blood circulation system disorder that results in an increase in blood pressure above the normal value or blood pressure of 140/90 mmHg. Risk factors for hypertension are divided into 2 groups, namely modifiable (smoking, excessive salt consumption, obesity, lack of physical activity, and stress) and non-modifiable (age, gender, and genetics). Hypertension can cause several complications such as heart disease, kidney failure, diabetes, and stroke. Handling hypertension includes pharmacology with antihypertensive drugs and non-pharmacological by living a healthy lifestyle. This study aimed to determine the factors associated with the incidence of hypertension in the community in Baet Lampuot village. Methods: Quantitative research with a cross sectional approach, sampling technique using non-probability sampling with accidental sampling technique. Data analysis used frequency distribution and chi square analysis. Results: Based on the results of the study, there was a relationship between the respondent's age and the risk of hypertension with a p value of 0.026 and there was a significant relationship between the sex of the respondent and the risk of hypertension with a p value of 0.024. Conclusion: There is a significant relationship between age and gender on the incidence of hypertension in Baet Lampuot desa village.Keywords: hypertension; age; gender
La fractocomposition en français et en tchèque : le cas de ‑gate
Authors: Mudrochová Radka , Koláříková Dagmar 
Published: January 2023

The objective of this contribution is to contrastively analyze the currency of words formed by the frac- tolexeme -gate in French and Czech languages. Before presenting the results of our research that relies especially on data derived from the SketchEngine (Czech Web 2017; French Web 2017) corpora, we find it necessary to address theoretical questions to frame the concept of fractocomposition in current linguistic studies. We have borrowed the term fractocomposition from the French linguist Jean-François Sablay- rolles (2019, p. 149), who defines it as a case of compounds “one of whose elements is a fractolexeme, a fragment of lexie which is valid for the entire lexie”. However, in our discussion we note also other terms that are also used to denote the same concept.
Profils du concept de Pologne dans la revue Polska en 1968. Étude des textes de propagande extérieure à destination des lecteurs du premier, du second et du tiers monde
Authors: Solová Regina 
Published: January 2023

This paper examines the profiles of the concept of Poland based on a corpus of foreign propaganda texts published in French and Slovak in 1968. The texts analysed come from three magazines: La Pologne, Polsko and La Revue Polonaise, created by political decision of the communist leaders to disseminate a positive image of Poland abroad. The purpose of this study is to test the hypothesis that the three profiles of the concept of Poland, aimed at first, second and third-world audiences, are different from each other due to differences motivated by extra-linguistic factors, and in particular, the various objectives of the country’s foreign policy. The study is inspired by the work of the Lublin School of Ethnolinguistics on the linguistic representation of reality. Its methods make it possible to identify variants of the representation of an object through the examination of textual data, such as the ways of naming it, the semantic relations of synonymy and antonymy, or the series of its aspects mobilised in the discourse.
Boomerang uniformity of power permutations and algebraic curves over 𝔽2n
Authors: Mesnager Sihem , Özbudak Ferruh 
Published: January 2023

AbstractWe obtain the Boomerang Connectivity Table of power permutationsF(x)=x2m−1 of F2n$F(x)={{x}^{{{2}^{m}}-1}}\text{ }\!\!~\!\!\text{ of }\!\!~\!\!\text{ }{{\mathbb{F}}_{{{2}^{n}}}}$withm∈{3,n−12,n+12,n−2}.$\left\{ 3,\frac{n-1}{2},\frac{n+1}{2},n-2 \right\}.$In particular, we obtain the Boomerang uniformity and the Boomerang uniformity set ofF(x) at b∈F2n∖F2.$F(x)\text{ }\!\!~\!\!\text{ at }\!\!~\!\!\text{ }b\in {{\mathbb{F}}_{{{2}^{n}}}}\setminus {{\mathbb{F}}_{2}}.$Moreover we determine the complete Boomerang distribution spectrum ofF(x) using the number of rational points of certain concrete algebraic curves overF2n.${{\mathbb{F}}_{{{2}^{n}}}}.$We also determine the distribution spectra of Boomerang uniformities explicitly.
A reliable and high throughput HPLC–HRMS method for the rapid screening of β-thalassemia and hemoglobinopathy in dried blood spots
Authors: Li Ziwei , Chen Deling , Shu Yan , Yang Jing , Zhang Juan , Ming wang  , Wan Kexing , Zhou Yinpin , He Xiaoyan , Zou Lin , Yu Chaowen 
Published: January 2023

Abstract

Objectives
Traditional methods for β-thalassemia screening usually rely on the structural integrity of hemoglobin (Hb), which can be affected by the hemolysis of red blood cells and Hb degradation. Here, we aim to develop a reliable and high throughput method for rapid detection of β-thalassemia using dried blood spots (DBS).

Methods
Hb components were extracted from a disc (3.2 mm diameter) punched from the DBS samples and digested by trypsin to produce a series of Hb-specific peptides. An analytical system combining high-resolution mass spectrometry and high-performance liquid chromatography was used for biomarker selection. The selected marker peptides were used to calculate delta/beta (δ/β) and beta-mutated/beta (βM/β) globin ratios for disease evaluation.

Results
Totally, 699 patients and 629 normal individuals, aged 3 days to 89 years, were recruited for method construction. Method assessment showed both the inter-assay and intra-assay relative standard deviation values were less than 10.8%, and the limits of quantitation for the proteo-specific peptides were quite low (1.0–5.0 μg/L). No appreciable matrix effects or carryover rates were observed. The extraction recoveries ranged from 93.8 to 128.7%, and the method was shown to be stable even when the samples were stored for 24 days. Prospective applications of this method in 909 participants also indicated good performance with a sensitivity of 100% and a specificity of 99.6%.

Conclusions
We have developed a fast, high throughput and reliable method for screening of β-thalassemia and hemoglobinopathy in children and adults, which is expected to be used as a first-line screening assay.
Health issues using 5G frequencies from an engineering perspective: Current review
Authors: Wersényi György 
Published: January 2023

Abstract
The possible adverse health effects of electromagnetic field (EMF) exposure have been in research focus since radio waves were introduced to telecommunication. Broadcast radio systems, satellites, and mobile communication devices use different bands of the radio spectrum, antennas, modulations, and radiated power. The proliferation of cellular networks and mobile phones as user devices have brought transmitting and receiving antennas in the close proximity of the human body and the head. Hundreds of experiments have been conducted to prove and disprove adverse health effects of exposure. Literature reviews of experimental results have also followed the current developments in technology; however, an exhaustive analysis performed on the methodologies has revealed many flaws and problems. This article focuses on the latest results on frequency bands mostly used for 5G below and above 6 GHz in the mmWave band. Current results do not indicate significant health effects and responses below the current safety limits. Nevertheless, further research directions can be identified, especially for mmWave radiation.