bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repressing miR-23a promotes the transdifferentiation of pancreatic α cells to β cells via negatively regulating the expression of SDF-1α
Authors: Lang Hongmei , Kang Chao , Chen Xiaorong , Xiang Jie , Lin Ning , Zhang Xingping 
Published: June 2023

Abstract
Pancreatic β-cell failure is a pathological feature in type 1 diabetes. One promising approach is the transdifferentiation of developmentally related pancreatic cell types, including glucagon-producing α cells. The chemokine stromal cell-derived factor-1 alpha (SDF-1α) is implicated in pancreatic α-to-β like cell transition. Here, the serum level of SDF-1α was lower in T1D with C-peptide loss, the miR-23a was negatively correlated with SDF-1α. We identify exosomal miR-23a secreted from β cells, functionally negatively regulate the expression of SDF-1α, followed by increased expression of Pax4 and decreased expression of Arx in vivo. Adenovirus-vectored miR-23a sponge and mimic were constructed to further explored the miR-23a on pancreatic α-to-β like cell transition in vitro, and the results were similar to cell experiments. Repression of miR-23a upregulated the level of insulin and downregulated the level of glucagon in αTCl-6 cells and STZ-induced diabetes mice models, promoting α-to-β like cell transition. Our results identified miR-23a as a new therapeutic target for the regeneration of pancreatic β cells from α cells.
SIRT1 Regulates Hepatocyte Programmed Cell Death via
GSDME – IL18 Axis in Human and Mouse Liver Transplantation
Authors: Kadono Kentaro , Kojima Hidenobu , Yao Siyuan , Kageyama Shoichi , Nakamura Kojiro , Hirao Hirofumi , Ito Takahiro , Dery Kenneth , Farmer Douglas , Kaldas Fady , Li Xiaoling , Kupiec-weglinski Jerzy 
Published: June 2023

Abstract
Sirtuin 1 (SIRT1) is a histone/protein deacetylase involved in cellular senescence, inflammation, and stress resistance. We previously reported that myeloid SIRT1 signaling regulates the inflamed liver’s canonical pyroptosis cell death pathway. However, whether/how hepatocyte SIRT1 is engaged in programmed cell death in the cold-stressed liver remains uncertain. Here, we undertook translational studies in human and mouse orthotopic liver transplantation (OLT) to interrogate the significance of hepatocyte-specific SIRT1 signaling in cold-stored donor livers and liver grafts after reperfusion. In the clinical arm of sixty human OLT patients, hepatic SIRT1 levels in cold-preserved donor livers correlated with anti-apoptotic Bcl-2 expression. After reperfusion, improved OLT function was accompanied by hepatic SIRT1 levels negatively associated with cleaved caspase-3 expression. In the experimental arm, we compared FLOX-control with hepatocyte-specific SIRT1-KO livers after orthotopic transplantation into WT mouse recipients, parallel with primary murine hepatocyte cultures subjected to cold activation with/without knockdown of SIRT1, GSDME, and IL18Rβ signaling. Hepatocyte SIRT1 deficiency upregulated apoptosis and GSDME-mediated programmed cell death, which in turn deteriorated the hepatocellular function and shortened OLT survival. Augmented GSDME processing, accompanied by increased secretion of IL18 by stressed hepatocytes, was prominent in SIRT1-deficient, cold-stored livers. Hepatocyte SIRT1 signaling regulated anti-apoptotic Bcl-2/XIAP proteins, suppressed cold stress-triggered apoptosis, and mitigated GSDME licensing to release IL18. Notably, while crosslinking IL18R depressed SIRT1 and Bcl-2/XIAP signaling in vitro, IL18 neutralization in vivo prevented hepatocellular damage and restored the anti-apoptotic phenotype in otherwise injury-prone SIRT1-deficient OLTs. In conclusion, this translational study identifies a novel hepatocyte SIRT1-IL18 signaling circuit as a therapeutic target in the mechanism underpinning hepatocyte death in human and mouse liver transplantation.
Efficacy of an Internet-based Self-help Intervention with Human Guidance or Automated Messages to Alleviate Loneliness: A Three-Armed Randomized Controlled Trial
Authors: Seewer Noëmi , Skoko Andrej , Käll Anton , Andersson Gerhard , Luhmann Maike , Berger Thomas , Krieger Tobias 
Published: June 2023

Abstract
Loneliness is a prevalent and stigmatized phenomenon associated with adverse (mental) health outcomes. However, evidence-based interventions to alleviate loneliness are scarce. This randomized controlled trial (ClinicalTrials.gov-ID: NCT04655196) evaluated the efficacy of an internet-based cognitive behavioral self-help intervention (ICBT) to reduce loneliness by comparing two intervention groups with guidance or automated messages against a waitlist control group. Adults (N = 243) suffering from loneliness were recruited from the general public and then randomly assigned (2:2:1) to a 10-week ICBT with human guidance (GU) or automated messages (AM) or to a waitlist control group (WL). Loneliness, assessed with the UCLA-9, was the primary outcome. Outcomes were assessed at baseline and 10 weeks (post) and analyzed using mixed-effects models. The pooled intervention conditions resulted in lower loneliness scores at post-assessment than the WL (Cohen’s d = 0.57, 95%-CI [0.25; 0.89]) and reduced depressive symptoms, social anxiety, social avoidance behavior, and rejection sensitivity (d = 0.32–0.52). The GU group had lower loneliness scores at post-assessment than the AM group (d = 0.42, 95%-CI [0.13; 0.70]). ICBT effectively alleviated loneliness, and guidance increased the reduction in loneliness compared to automated messages. Alleviating loneliness with ICBT further seems to reduce the overall burden of psychopathological symptoms.
“Evolution” of intravascular leiomyomatosis
Authors: Chen Li , Yang Yunping , Zhao Chengzhi 
Published: June 2023

Abstract
Background
Intravenous leiomyomatosis (IVL) is a rare and specific type of smooth muscle tumor that is histologically benign but has a malignant biological behavior. It is commonly associated with a history of uterine leiomyomas.
Case presentation:
A 36-year-old woman, G1P1, presented to the hospital with left lower abdominal pain for 2 months and she has accepted hysteroscopic myomectomy about 1 year ago. Ultrasound venography, echocardiography and computed tomography venography (CTV) of inferior vena cava were performed,which revealed IVL located in left intramural myometrium walls growing along the left ovarian vein reaching the level of the lumbar 5-sacral 1 disc. Laparoscopic bilateral salpingo-oophorectomy and hysterectomyis were scheduled. The IVL in the left ovarian vein and parauterine venous plexus were detected and excised completely during surgery. IVL was diagnosed by postoperative pathology and immunohistochemistry. The patient recovered well after surgery.No surgical-related or anesthesia-related complications occurred.The 3-month follow-up CTV of inferior vena cava and echocardiography examination revealed normal.
Conclusions
The cause of IVL is unknown, this observation demonstrates that hysteroscopic myomectomy might lead to the occurrence of IVL.
In vitro action of papaya (Carica papaya) latex and pure papain against oocysts of Eimeria bovis
Authors: de Siqueira Lucas Nogueira , Mamani Ruth Celestina Condori , FIGUEROA LISSETH BIBIANA PUENTES , de Souza Debora Castro , Albuquerque Laísa Bastos , Moreira Tiago Facury , Braga Fabio Ribeiro , Soares Filippe Elias de Freitas 
Published: June 2023

Abstract

Purpose: Eimeria bovis is a protozoan that causes coccidiosis, a disease with a great impact on the world cattle industry. However, its control has not been fully efficient and, therefore, the search for alternative measures of parasitic and or even environmental control of the infecting forms (sporulated oocysts) is welcome. Thus, the aim of this study was to evaluate the effect of papaya (Carica papaya) latex and pure papain on sporulated and non-sporulated oocysts of E. bovis.
Methods: Oocysts were collected from the feces of calves previously inoculated with E. bovis and stored in potassium dichromate solution. Then, the oocysts were evaluated for the application of aqueous solutions (active and denatured) of latex and papain at concentrations 10, 15 and 30% (w/v), in addition to the control group (water), which were incubated at 28 °C for 48 hours.
Results:There was a reduction in oocysts in the treatments compared to the control group. However, only at the concentration of 30% (m/v) of papain after incubation for 48 hours at 28°C there was a significant difference (p<0.01) in relation to the control.
Conclusion:The results show that the use of papain-rich C. papaya extracts has great future prospects in controlling E. bovis.
Open reduction and internal fixation by hollow screw for capitellum fractures in children
Authors: Zhao Ren-huan , Liu Xin , Xie Li-wei , Zhou Ying , Deng Zhi-qiang , Chen Wei , Kang Chi , Ye Jia-jun 
Published: June 2023

Abstract
Background
To evaluate the clinical outcome of capitellum fractures in children treated with open reduction and internal fixation (ORIF) by hollow screw.
Methods
Five children with capitellum fractures, aged 10–14 years, average age = 12.2 years, who were admitted to our hospital between January 2018 and June 2021, were included in this study. All patients underwent ORIF using compressive hollow screws inserted in a posterior-to-anterior direction. The Broberg-Morrey elbow function score was used to evaluate the outcome during the follow-up.
Results
The mean follow-up period was 11.8 (9–18) months. The mean Elbow Performance Index score was 97 (range 85–100), and the scores were excellent in four cases and general in one case. All patients achieved anatomic bone union and good elbow flexion and extension functions, no patient developed vascular and nerve injury, infection or avascular necrosis.
Conclusion
In the treatment of children with capitellum fractures, the lateral approach of open reduction and hollow-screw posterior-to-anterior internal fixation has a good effect.
Simulation-based assessment of the performance of hierarchical abundance estimators for camera trap surveys
Authors: Martijn Bollen , Jim Casaer , Natalie Beenaerts , Thomas Neyens 
Published: June 2023

Abstract
Knowledge on animal abundances is essential in ecology, but is complicated by low detectability of many species. This has led to a widespread use of hierarchical models (HMs) for species abundance, which are also commonly applied in the context of nature areas studied by camera traps. However, the best choice among these models is unclear, particularly based on how they perform in the face of complicating features of realistic populations, including: movements relative to sites, multiple detections of unmarked individuals within a single survey, and low detectability. We conducted a simulation-based comparison of three HMs (Royle-Nichols, binomial N-mixture and Poisson N-mixture model) by generating groups of individuals moving according to a bivariate Ornstein-Uhlenbeck process, and monitored by camera traps. Under a range of simulated scenarios, none of the HMs consistently yielded accurate abundances. Yet, the Poisson N-mixture model performed well when animals did move across sites, despite accidental double counting of individuals. Absolute abundances were better captured by Royle-Nichols and Poisson N-mixture models, while a binomial N-mixture model better estimated the actual number of individuals that used a site. Focusing on relative trends in abundance improved the performance of all HMs, and were captured with similar accuracy across these models.
Chlorinated paraffins in food contact materials made of rubber from the German market
Authors: Vetter Walter , Schulz Tobias , Schweizer Sina , Zellmer Sebastian 
Published: June 2023

Abstract
Chlorinated paraffins (CPs), also known as polychlorinated alkanes (PCA), are a very complex class of polyhalogenated compounds currently produced in unmatchable amounts for different industrial uses. Here, we investigated the presence of CPs in 21 rubber samples used as food contact materials from the German market. For this purpose, an analytical method was developed which consisted of (i) freezing and (ii) grounding of the material, (iii) removal of oligomers by adsorption chromatography, (iv) matrix destruction with sulfuric acid, and (v) GC/ECNI-MS screening and quantification. Three of the rubber samples were found to contain predominantly medium-chain CPs (MCCPs; C14- to C17-CPs) at 69, 113,000 and 341,000 mg/kg. The highest amount (corresponding with 34 weight-% CPs) was determined in one rubber band sample used to bundle a bunch of spring onions. A very high CP content of >300,000 mg/kg in this rubber band sample was confirmed by quantitative 1H NMR (qNMR) analysis. This rubber material was in direct contact with the onions and leaching of CPs into the food cannot be excluded. Even more, micro-abrasions of rubber could be unintentionally swallowed by the consumer and this CP exposure could be higher than the current CP intake via food.
Single-port three-dimensional (3D) videoscope assisted endoscopic breast surgery-Preliminary results and patient-reported satisfaction in 156 breast cancer and gynecomastia cases
Authors: Chia Clement Luck Khng , Sae-lim Chayanee , Lai Hung-Wen , Chandrachamnong Korawan , Huang Hsin-I , Chen Dar-Ren , Chen Shou-Tung 
Published: June 2023

Abstract
Background
Minimal-accessed (robotic and endoscopic) breast cancer surgery increasingly performed due to better cosmetic results and acceptable oncological outcomes. This study aims to demonstrate the clinical safety and patient-reported cosmetic satisfaction of single-port three-dimensional (3D) videoscope assisted endoscopic breast surgery (EABS), which is our new endoscopic surgical innovation, in both malignant and benign breast conditions.
Methods
Patients undergone single-port 3D EABS from 1 August 2018 to 31 July 2022 in a single institution were enrolled. Clinical outcomes of this procedure were retrospectively reviewed, the patient-reported cosmetic satisfaction was evaluated by a questionnaire and reported herein.
Results
During the study period, 156 patients underwent 180 procedures of single port 3D EABS. 166 (92.2%) procedures were 3D endoscopic-assisted nipple-sparing mastectomy (3D E-NSM; 132 therapeutic procedures for breast cancer, 14 prophylactic contralateral mastectomies, 20 procedures for gynecomastia). 14 (7.8%) procedures of 3D endoscopic-assisted breast conserving surgery (3D E-BCS) were performed (12 3D E-BCS, 2 3D E-BCS with 3D videoscope assisted partial breast reconstruction, which was 1 case with latissimus dorsi flap and 1 case with omental flap). Mean operative time was 244 ± 110 minutes in 3D E-NSM and 260 ± 142 minutes in 3D E-BCS. Mean intra-operative blood loss was 50.2 ± 46.5 ml in 3D E-NSM and 32.8 ± 17.5ml in 3D E-BCS. Subnipple biopsy showed positive malignancy in 7 (5.4%) 3D E-NSM patients. None of the 3D E-BCS patients found margin involvement, however, those reported in 3 (2.6%) 3D E-NSM patients. Complications were found in 32 (22%) patients; 7 (4.8%) transient nipple-areolar complex (NAC) ischemia, 7 (4.8%) partial NAC necrosis, 1 (0.7%) total NAC necrosis, and 1 (0.7%) implant loss. During the mean follow up time of 27 months, there were 6 (4.7%) patients with locoregional recurrence, 7 (5.4%) distant metastasis, and 1 (0.7%) mortality. 89.3% (67/75) of patients answering the cosmetic-evaluated questionnaire reported good and excellent satisfaction.
Conclusions
Single-port 3D EABS is a novel surgical innovation, which is able to perform safely in either malignant or benign breast conditions and offer promising cosmetic results.
Correlation of ABO blood groups and salivary molecular markers interleukin-1β and sclerostin in patients with stage III grade A periodontitis - A clinico-biochemical study
Authors: Pradeep AR , Coutinho Amita , Nair Lekshmi S 
Published: June 2023

Abstract
Objective
The objective of this study’s was to estimate salivary interleukin-1β(IL-1β) and sclerostin(SOST) and correlate it with ABO blood groups in Stage III Grade A periodontitis.
Materials and Methods
88 subjects were recruited for this study- 44 healthy subjects(G1) and 44 subjects with Stage III Grade A periodontitis(G2). Based on the blood groups the subjects were then divided equally into subgroups containing 11 subjects each. Plaque index(PI), bleeding index(BI), gingival index(GI), probing depth(PD) and interdental clinical attachment loss(ICAL) were the clinical parameters recorded. Salivary samples were collected, and ELISA kits were used to quantify the levels of IL-1β and SOST.
Results
A statistically significant difference was seen between G1 and G2 on comparing PI, BI, GI, PD,ICAL, IL-1β and SOST and these parameters were significantly higher in G2 compared to G1 (P < 0.05). The salivary IL-1β levels in G2 a statistically significant difference among the different blood groups. IL-1β was significantly less in blood type AB as compared to O, B, A. Whereas SOST showed no statistically significant difference among the blood groups.
Conclusion
SOST and salivary IL-1β can be considered as potential indicators of periodontal disease. Individuals with blood type AB had considerably lower levels of salivary IL-1β, which may have an impact on the severity and progression of periodontal disease. Hence ABO blood groups can be considered as a genetic host factor to determine the risk of an individual to periodontal disease.
Clinical Relevance
The ABO blood types can be viewed as a hereditary risk factor if linked to periodontal disease. This information can aid in the development of early treatment plans for highly susceptible individuals and focus on regions that don't respond to periodontal therapy
Diagnostic and prognostic values of tsRNAs in lung cancer: A meta-analysis
Authors: Tang Cheng , Sun Su-Xia , Gu Chao , Li Chao-Juan , Xu Jin , Su Ke-Lei , Zhou Dan-Dan , Yu Kuai , Xiao Qing-Ling , Chen Xiao-Li 
Published: June 2023

Abstract

Background
Lung cancer (LC) is the leading cause of cancer death in humans. tRNA-derived small RNA (tsRNA) is a novel biomarker that plays a crucial role in the genesis and development of LC. In this study, we aimed to investigate the value of differentially expressed tsRNA in LC through meta-analysis.
Methods
PubMed and Web of Science were searched until March 31, 2023. Diagnostic odds ratios (DORs) and area under the curves (AUCs) were used to evaluate the potential of tsRNAs as diagnostic markers for LC. Furthermore, hazard ratios (HRs) and 95% confidence intervals (95%CIs) were used to analyze the association of tsRNAs with LC prognosis.
Results
A total of 10 studies were included for analysis. Our results indicated that the combined DOR of total tsRNAs in LC diagnosis was 5.45, and AUC was 0.76. Subgroup analysis showed that high expression of tsRNAs in serum had higher diagnostic efficacy (DOR = 15.94, AUC = 0.87). Moreover, high expression of tsRNAs was associated with a worse prognosis in LC patients (HR = 1.59, 95%CI: 1.33–1.90).
Conclusion
Our findings suggest that high expression of tsRNAs has potential value in the diagnosis and prognosis of LC patients. However, further high-quality studies are needed to validate our results.
Effect of Ultrasound Therapy on Postpartum Uterine Involution: A Randomized Controlled Trial
Authors: Wei Dongmei , Wang Zhijian , Yue Jun , Meng Jian , Niu Xiaoyu 
Published: June 2023

Abstract
Background
Short-term poor uterine involution manifests as uterine contraction weakness. This is one of the important causes of postpartum hemorrhage and is a serious threat to the life and safety of the mother. To investigate whether low-intensity focused ultrasound (LIFUS) could shorten lochia duration, relieve postpartum, and accelerate uterine involution compared with the sham treatment.
Methods
A multicenter, concealed, randomized, blinded, and sham-controlled clinical trial was conducted at three medical centers with 176 subjects, using a parallel group design. Subjects were enrolled from October 2019 to September 2020 and followed up for 42 days. The subjects who had normal prenatal checkups and met the inclusion and exclusion criteria were randomly allocated into a LIFUS or a sham group with a random sequence generated by a computer. Patients in the LIFUS group were given usual care with LIFUS treatmet, in which a LIFUS signal was transmitted to the uterus site through coupling gel, or sham treatment, in which low-intensity ultrasound signal output was not present. The duration of lochia, measured by weekly telephonic follow-ups after discharge, was the primary outcome. The involution of the uterus, determined by measuring the height of the fundus of the uterus, was the secondary outcome.
Results
Of the 256 subjects screened for eligibility, 176 were enrolled and randomly divided into the LIFUS group (n = 88) or the sham group (n = 88). Data on the height of the uterine fundus was derived from all the patients and 696/704 (99%) measurements were taken. Overall, a statistically significant difference was noted in time to lochia termination (hazard ratio: 2.65; 95% confidence interval [CI]: 1.82–3.85; P < 0.05). The height of the fundus showed significant difference following the second (mean difference: −1.74; 95% CI: −1.23 to − 2.25; P < 0.05) and the third treatment session (mean difference: −3.26; 95% CI: −2.74 to − 3.78; P < 0.05) after delivery. None of the subjects had any adverse reactions, such as skin damage and allergy during treatment.
Conclusions
This study revealed that LIFUS therapy could be considered as safe and effective. Additionally, it contributed to uterine involution and reduced the duration of postpartum lochia.
Shared Molecular Mechanisms Between Primary open angle glaucoma and Atherosclerosis via Integrated Bioinformatic Analysis
Authors: Sun Yaru , Liu Qingping , Zhang Mingzhi 
Published: June 2023

Abstract
This study aims to explore the shared hub genes and molecular mechanisms between primary open angle glaucoma (POAG) and atherosclerosis (AS) through integrated bioinformatic analysis. Data were downloaded from the Gene Expression Omnibus database. Ninety-two common genes were identified through the differentially expressed genes (DEGs) analysis and Weighted Gene Co-Expression Network Analysis (WGCNA) between POAG and AS. According to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the common genes were enriched in oxidative stress and inflammatory response. Then, through the protein-protein interaction (PPI) network and ROC curve analysis, four shared hub genes were identified and the Gene Set Enrichment Analysis (GSEA) indicated these were all highly enriched in inflammatory and immune response. In addition, eight miRNAs and six transcription factors (TFs) were predicted to be key miRNAs and TFs. Finally, the Comparative Toxicogenomics Database identified twenty potential small-molecule drugs. This study revealed shared hub genes and molecular mechanisms between POAG and AS. Oxidative stress, the inflammatory and immune response may be a common contributor to POAG and AS. These common hub genes and molecular mechanisms may offer promising clues for further experimental studies.