bluuebunny/crossref_metadata_2025_split huggingface dataset:
Second-trimester Uterine Artery Doppler Pulsatility Index in Singleton
Pregnant Women with and without Risk of Pre-eclampsia
Authors: Shojaei Kobra , Elahian Shima , Saadati Najmieh , Jafari Razieh Mohammad , Gharibvand Mohammad Momen , Seyedtabib Maryam , Mohammadi Shooka 
Published: August 2023

Background:
Preeclampsia (PE) is gestational hypertension and one of the most serious
disorders in pregnant women.

Objective:
This study aimed to compare second-trimester uterine artery Doppler pulsatility index (UAPI) in singleton pregnant women with and without risk of PE.

Methods:
A case-control study was carried out among 200 singleton pregnant women in the second
trimester of pregnancy, who were referred to Imam Hospital in Ahvaz (Iran). The data were recorded
on their demographic, obstetric, clinical characteristics, and Doppler indices. The average UA-PI value was considered as the mean PI between the right and left arteries. Women who were categorized
into Groups 1 and 2 had a moderate-high and low risk of PE, respectively.

Results:
The mean UA-PI, right UA-PI, left UA-PI, systolic and diastolic blood pressures (BP), age,
body mass index (BMI), and abdominal circumference (AC) of women in Group 1 were significantly
higher than those in Group 2 (p< 0.05). Furthermore, Group 1 had a higher frequency of abortions,
comorbidities, abnormal UA Doppler results, and nulliparous women than Group 2 (p< 0.05). Based
on maternal age (<35, ≥35), BMI (<25, ≥25), comorbidities (yes, no), and nulliparity (yes, no) categories, those who were in Group 1 had a significantly higher mean systolic and diastolic BP, UA-PI,
right UA-PI, and left UA-PI than their counterparts in Group 2 (P< 0.001).

Conclusion:
This study declared the second-trimester UA-PI had good potential for timely prediction
of PE risk in pregnant women.
A Diagnostic Tool for Good Chromatographic Practices Applied to HPLC
in Pharmaceutical Quality Control
Authors: Menezes Gama Rodolpho Guilherme , Ramos Aline de Souza , Amaral Ana Claudia Fernandes 
Published: August 2023

Background:
High-performance liquid chromatography is one of the most used analytical
techniques in quality control in the pharmaceutical industry. Since it is a complex technique, it needs
good practices that can contribute to compliance with regulatory requirements.

Objetive:
This study aims to establish a diagnostic tool for Good Chromatographic Practices (GCP)
for the self-assessment of a Quality Control Laboratory (QCL).

Methods:
The research was carried out on scientific bases, pharmaceutical legislation, as well as
guides published by manufacturers.

Results:
Seven axes of action were identified: implementation, management, and continuous improvement
of GCP in the laboratory; GCP in the installation, operationalization, qualification, and
validation processes of the equipment and software; GCP in processes related to data management,
including guidelines regarding access, generation, integrity, and traceability; GCP related to the management
and use of consumables; GCP related to handling, maintenance, analytical and operational
troubleshooting; GCP in the processes of preparation, use, and storage of analytical solutions and reagent
solutions; and GCP related to the acquisition and processing of standards, samples, and results.
These axes resulted in a diagnostic tool with 124 questions.

Conclusion:
The application of the GCP diagnostic tool provides the mapping of the routine and procedures
related to the execution of the HPLC technique for quality control in the pharmaceutical industry,
contributing to meeting regulatory requirements.
Application of a Quantitative Proton Nuclear Magnetic Resonance
Method for the Determination of Protopine in Radix Dactylicapnotis
Authors: Li Xue-Jiao , Dong Jian-Wei , Liu Zheng-Fen , Shi Jun-You , Zhang Feng-Mei , Fa Yan-Mei , Li Ya-Li , Wang Xue-Xian 
Published: August 2023

Background:
The Bai ethnologic herb Radix Dactylicapnotis, the root and tuber of Dactylicapnos
scandens (Papaveraceae), is used for clearing heat, relieving pain, and achieving hemostasis
and antihypertensive effects.

Objective:
The study aimed to develop a quantitative method for determining the protopine content in
Radix Dactylicapnotis by using proton nuclear magnetic resonance (1H NMR) spectroscopy.

Methods:
The deuterium solvent, internal standard, and NMR parameters were optimized. The quantitative
method was validated by linearity, precision, accuracy, repeatability, and stability, as well as
limit-of-detection (LOD) and limit-of-quantitation (LOQ) assays.

Results:
A mixture solution consisting of 500 μL of DMSO-d6 and 20 μL of D2O enabled satisfactory
separation of the signals to be integrated into the 1H NMR spectrum. Trimethyl benzene-1,3,5-
tricarboxylate (TMBT) was selected as an internal standard. The integration of δ 6.05-6.08 corresponding
to OCH2O was selected to quantify protopine. The developed quantitative method was
found to be precise and accurate and to exhibit excellent linearity and range. The protopine content in
Radix Dactylicapnotis could be quantified accurately using the featured signal.

Conclusion:
This is the first study to report quantitative 1H NMR determination of protopine in Radix
Dactylicapnotis. The study results indicate that quantitative 1H NMR represents a feasible alternative
to HPLC-based methods for the quantitation of protopine in Radix Dactylicapnotis, and is suitable for
the quality control of Radix Dactylicapnotis.
Efficacy and Safety of Bone Marrow Derived Stem Cell Therapy for
Ischemic Stroke: Evidence from Network Meta-analysis
Authors: Wang Xing , Yang Jingguo , You Chao , Bao Xinjie , Ma Lu 
Published: August 2023

Background:
Several types of stem cells are available for the treatment of stroke patients. However, the optimal type of stem cell remains unclear.

Objective:
To analyze the effects of bone marrow-derived stem cell therapy in patients with ischemic stroke by integrating all available direct and indirect evidence in network meta-analyses.

Methods:
We searched several databases to identify randomized clinical trials comparing clinical outcomes of bone marrow-derived stem cell therapy vs. conventional treatment in stroke patients. Pooled relative risks (RRs) and mean differences (MDs) were reported. The surface under the cumulative ranking (SUCRA) was used to rank the probabilities of each agent regarding different outcomes.

Results:
Overall, 11 trials with 576 patients were eligible for analysis. Three different therapies, including mesenchymal stem cells (MSCs), mononuclear stem cells (MNCs), and multipotent adult progenitor cells (MAPCs), were assessed. The direct analysis demonstrated that stem cell therapy was associated with significantly reduced all-cause mortality rates (RR 0.55, 95% CI 0.33 to 0.93; I2=0%). Network analysis demonstrated MSCs ranked first in reducing mortality (RR 0.42, 95% CrI 0.15 to 0.86) and improving modified Rankin Scale score (MD -0.59 95% CI -1.09 to -0.09), with SUCRA values 80%, and 98%, respectively. Subgroup analysis showed intravenous transplantation was superior to conventional therapy in reducing all-cause mortality (RR 0.53, 95% CrI 0.29 to 0.88).

Conclusion:
Using stem cell transplantation was associated with reduced risk of death and improved functional outcomes in patients with ischemic stroke. Additional large trials are warranted to provide more conclusive evidence.
Bone Mineral Density is Related to CD4+ T Cell Counts and Muscle Mass
is Associated with B Cells in Common Variable Immunodeficiency Patients
Authors: de Melo Daniel Barreto , Pereira Rosa Maria Rodrigues , Sini Bruno , Levy Débora , Takayama Lilian , Kokron Cristina Maria , Berselli Marinho Ana Karolina , Grecco Octavio , Kalil Filho Jorge Elias , Barros Myrthes Toledo 
Published: August 2023

Background:
Common variable immunodeficiency (CVID) is a primary immunodeficiency characterized by chronic/recurrent respiratory infections, bronchiectasis, autoimmunity, inflammatory, gastrointestinal diseases and malignancies associated with a chronic inflammatory state and increased risk of osteoporosis and muscle loss.

Aim:
The aim of this study was to evaluate bone mineral density (BMD), body composition and their relationship with lymphocyte subpopulations in CVID patients.

Methods:
Dual-energy X-ray absorptiometry was performed to assess BMD, lean mass, and fat mass in CVID patients. Peripheral blood CD4+, CD8+, and CD19+ cells were measured using flow cytometry.

Results:
Thirty-three patients (37.3 ± 10.8 years old) were examined. Although only 11.8% of the in-dividuals were malnourished (BMI <18.5 kg/m2), 27.7% of them had low skeletal muscle mass index (SMI), and 57.6% of them had low BMD. Patients with osteopenia/osteoporosis presented lower weight (p = 0.007), lean mass (p = 0.011), appendicular lean mass (p = 0.011), SMI (p = 0.017), and CD4+ count (p = 0.030). Regression models showed a positive association between CD4+ count and bone/muscle parameters, whereas CD19+ B cell count was only associated with muscle variables. Analysis of ROC curves indicated a cutoff value of CD4+ count (657 cells/mm3; AUC: 0.71, 95% CI 0.52-0.90) which was related to low BMD. Weight (p = 0.004), lean mass (p = 0.027), appendicular lean mass (p = 0.022), SMI (p = 0.029), total bone mineral content (p = 0.005), lumbar (p = 0.005), femoral neck (p = 0.035), and total hip BMD (p<0.001) were found to be lower in patients with CD4+ count below the cutoff.

Conclusion:
CVID patients presented with low BMD, which was associated with CD4+ count. More-over, low muscle parameters were correlated with B cell count.
Antioxidant Activity of Medicinal Plants and Herbs of North Aegean,
Greece: Current Clinical Evidence and Future Perspectives
Authors: Poulios Efthymios , Vasios Georgios K. , Psara Evmorfia , Antasouras Georgios , Gialeli Maria , Pavlidou Eleni , Tsantili-Kakoulidou Anna , Troumbis Andreas Y. , Giaginis Constantinos 
Published: August 2023

Background:
Medicinal plants and herbs constitute rich sources of flavoring, and aromatic
compounds, namely phytochemicals, which have many positive impacts on human health, such as antioxidant,
anticancer, antimicrobial, anti-inflammatory, cardioprotective, and neuroprotective properties. Such
bioactive compounds may be considered lead compounds that can be introduced in the drug design process
to obtain novel drug candidates with better bioavailability and therapeutic efficiency. The Islands of
the North Aegean have rich biodiversity and many medicinal plants and herbs with multiple health benefits.

Objective:
This study aims to summarize and scrutinize the antioxidant activity of medicinal plants and
herbs of the North Aegean islands.

Methods:
A thorough search of the existing literature was performed in the most accurate scientific databases,
using a set of effective and relative keywords and including only clinical human studies written in
English.

Results:
Several clinical studies have highlighted the potential antioxidant activity of phytochemicals
from plants such as St. John's wort, chamomile, rosemary, spearmint, mastiha, mountain tea, oregano,
sage, and thyme, as measured in the blood and saliva of human individuals, after administration of extracts
and solutions of these plants. Decreased levels of oxidized lipoproteins, increased activity of antioxidant
enzymes, and an enhanced total antioxidant capacity were observed.

Conclusions:
Current clinical trials remain still limited, and an enhanced series is needed with a better
methodology design, larger sample size, longer intervention periods, using controlled groups and randomization
to unravel the antioxidant activity of these North Aegean endemic plants in human individuals.
The Sequelae of Sleeping Issues and their Management in Post COVID-
19: A Unani and Contemporary Narrative Review
Authors: Jamal Anwar , Siddiqui Aisha , Akhlaq Shaheen , Ara Shabnam Anjum , Itrat Malik 
Published: August 2023

Background:
The Coronavirus disease (COVID-19) impacted not only physical health
but also psychological health, including anxiety, depressive symptoms, and sleep issues. Disrupted
sleep has now become a global concern and burden in the post-COVID period of recovery. The
Unani system of medicine elaborated on sleeping issues and their management during the pandemic.
However, the claims of the Unani system and its medications in improving the various domains
of sleep quality during the revival period have not been investigated.

Materials and Methods:
A search of electronic databases on Pub Med, Scopus, Web of Science,
and CINAHL, on sleep disorders, post-COVID-19 and a manual search of Unani classical text related
to sleep quality improvement methods was conducted.

Results:
Recent scientific evidence has found a positive association between COVID-19 and sleep
disorders. Unani medicine suggests improvement of sleep issues by maintaining six essential factors,
dieto-therapy, pharmacotherapy, and regimens (like Nutool (irrigation), Hamam (Turkish
bath), and Dalk (massage). Several pre-clinical and clinical trials done on Unani herbs (single and
poly-herbal preparations) have elucidated the scientific rationale for improving sleep quality.

Conclusion:
Unani therapy has sufficient potential for improving sleep outcomes for COVID-19
patients as they are efficacious, safe and affordable therapeutic options. Additionally, there is still a
need for evidence to support the validity of Unani psychotherapy as a multifaceted and holistic
approach.
BDNF and Cerebellar Ataxia
Authors: Lalonde Robert , Hernandez Magali , Strazielle Catherine 
Published: August 2023

Abstract:
Brain-derived neurotrophic factor (BDNF) has been proposed as a treatment for neurodegeneration,
including diseases of the cerebellum, where BDNF levels or those of its main receptor,
TrkB, are often diminished relative to controls, thereby serving as replacement therapy. Experimental
evidence indicates that BDNF signaling countered cerebellar degeneration, sensorimotor
deficits, or both, in transgenic ATXN1 mice mutated for ataxin-1, Cacna1a knock-in mice mutated
for ataxin-6, mice injected with lentivectors encoding RNA sequences against human FXN
into the cerebellar cortex, Kcnj6Wv (Weaver) mutant mice with granule cell degeneration, and rats
with olivocerebellar transaction, similar to a BDNF-overexpressing transgenic line interbred with
Cacng2stg mutant mice. In this regard, this study discusses whether BDNF is effective in cerebellar
pathologies where BDNF levels are normal and whether it is effective in cases with combined
cerebellar and basal ganglia damage.
Progress in Superhydrophobic Structure Optimisation and Performance Enhancement: A Review
Authors: Tang Jinjing , Yang Daolong , Xing Bangsheng , Duan Haichao , Li Zijie 
Published: August 2023

Background:
Superhydrophobic surfaces have broad application prospects in
several fields due to their excellent hydrophobic properties, but the traditional methods
of manufacturing superhydrophobic surfaces are time-consuming and laborious, the surface
wear resistance is poor, and the chemical reagents contain toxic substances, making
it difficult to promote the use of superhydrophobicity inexpensively.

Objective:
To solve the problems of high cost, instability, and poor mechanical properties
of superhydrophobic structures, we explore the preparation methods of superhydrophobic
surface structures to improve the surface superhydrophobicity and reduce manufacturing
and usage costs.

Method:
This paper provides an overview of the literature on preparing superhydrophobic
structures and improving superhydrophobic properties. Based on the summary of
the research results of other scholars, this paper focuses on the preparation of superhydrophobic
surfaces by carbine-co-polymerization covalent grafting chemical reactions
and the improvement of superhydrophobic properties by durable opaque coatings with
vacuum-deposited layers.

Results:
These two methods are simple to operate and circumvent the problem of oxidative
degradation of compounds in the natural environment to produce environmental
pollutants. The method I produces a low surface energy stratified micro-nano composite
structure on the fiber surface of the fabric by carbon copolymerization covalent grafting
reaction. The method II prepares a durable opaque coating with a vacuum-deposited
layer, particularly useful on mechanical components or for any other applications not
requiring an optically clear coating.

Conclusion:
This paper provides an important basis for optimizing the preparation process
of superhydrophobic structures, synthesizing and developing environmentally
friendly superhydrophobic materials, extending the service life of superhydrophobic
materials, and provides specific guidance for improving the superhydrophobic properties
and durability and enhancing the combination of superhydrophobic surfaces with
additional functions.
Analytical Method Development, Validation and Forced Degradation
Study of Dapagliflozin by RP-HPLC
Authors: Chaudhari Ujwal , Sahu Jagdish K. , Dande Payal R. 
Published: August 2023

Background:
Worldwide, it is projected that 285 million individuals have diabetes, and by 2030, this number is expected to climb to 438 million. About 90% of cases of diabetes mellitus are type 2 (T2DM). Insulin sensitizers, such as metformin and thiazolidinediones; insulin secreta-gogues, such as sulfonylureas and glinides; dipeptidyl peptidase 4 (DPP-4) inhibitors; glucosidase inhibitors, or oral combination therapy are currently available treatments for type 2 diabetes. Some of these drugs exhibit serious limitations; thus, it is crucial to design an innovative therapy that is efficient and depends on a new channel.

Aim:
In the current work, a stability-indicating reverse phase HPLC (RP-HPLC) technique was developed and subsequently validated for the detection of dapagliflozin in its API.

Methods:
The stability-indicating HPLC method for assay included the use of Kromasil 100-5-C8 (100 mm × 4.6 mm) column, UV detector 224 nm, mobile phase composition involving a mixture of acetonitrile:water (52:48), and a flow rate of 1.0 mL/min. ICH guidelines were followed for the method's validation. To assess the method's specificity and stability in showing characteristics, stress degradation studies were carried out. The working standard solution of dapagliflozin was exposed to 1 and 2 N HCl by refluxing 1 and 2 N NaOH with 30% hydrogen peroxide by volume and UV radiation in order to conduct a degradation study.

Results:
All system suitability parameters were determined to be within the intended ranges, and the drug's retention duration was discovered to be 1.67 minutes. It was also investigated as to how the drug degraded under various circumstances. The drug was discovered to be stable under situations of photolytic, thermal, neutral, alkaline, and oxidative deterioration. The developed stability-indicating HPLC technique was validated in accordance with ICH Q2 recommendations, and the validation parameters, such as linearity, precision, and robustness, were achieved within the ap-proved standards.

Conclusion:
It may be concluded that this method is stability-indicating and specific, and it can be successfully applied to analyze tablet dosage forms containing dapagliflozin.
Trends in the Baidu Index in Search Activity Related to Mpox at Geographical and Economic Levels and Associated Factors in China: National Longitudinal Analysis
Authors: Du Min , Yan Wenxin , Zhu Lin , Liang Wannian , Liu Min , Liu Jue 
Published: August 2023

Background
Research assessing trends in online search activity related to mpox in China is scarce.

Objective
We aimed to provide evidence for an overview of online information searching during an infectious disease outbreak by analyzing trends in online search activity related to mpox at geographical and economic levels in China and explore influencing factors.

Methods
We used the Baidu index to present online search activity related to mpox from May 19 to September 19, 2022. Segmented interrupted time-series analysis was used to estimate trends in online search activity. Factors influencing these trends were analyzed using a general linear regression (GLM) model. We calculated the concentration index to measure economic-related inequality in online search activity and related trends.

Results
Online search activity was highest on the day the first imported case of mpox appeared in Chongqing compared to 3 other cutoff time points. After the day of the first imported mpox case in Taiwan, the declaration of a public health emergency of international concern, the first imported mpox case in Hong Kong, and the first imported mpox case in Chongqing, national online search activity increased by 0.642%, 1.035%, 1.199%, and 2.023%, respectively. The eastern regions had higher increases than the central and western regions. Across 31 provinces, municipalities, and autonomous regions, the top 3 areas with higher increases were Beijing, Shanghai, and Tianjin at 3 time points, with the exception of the day of the first imported mpox case in Chongqing (Chongqing replaced Tianjin on that day). When AIDS incidence increased by 1 per 100,000 people, there was an increase after the day of the first imported mpox case in Chongqing of 36.22% (95% CI 3.29%-69.15%; P=.04) after controlling for other covariates. Online search activity (concentration index=0.18; P<.001) was more concentrated among populations with a higher economic status. Unlike the central area, the eastern (concentration index=0.234; P<.001) and western areas (concentration index=0.047; P=.04) had significant economic-related disparities (P for difference <.001) in online search activity. The overall concentration index of changes in online search activity became lower over time.

Conclusions
Regions with a higher economic level showed more interest in mpox, especially Beijing and Shanghai. After the day of the first imported mpox case in Chongqing, changes in online search activity were affected by AIDS incidence rate. Economic-related disparities in changes in online search activity became lower over time. It would be desirable to construct a reliable information source in regions with a higher economic level and higher AIDS incidence rate and promote public knowledge in regions with a lower economic level in China, especially after important public events.