bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Extracorporeal Shockwave therapy on pain and function in Myofascial Pain Syndrome: A systematic review and meta-analysis of randomized clinical trials
Authors: Avendaño-López Carlos , Megía-García Álvaro , Beltran-Alacreu Hector , Serrano-Muñoz Diego , Arroyo-Fernández Rubén , Comino-Suárez Natalia , Avendaño-Coy Juan 
Published: May 2023

ABSTRACT

Objective
To examine the effectiveness of ESWT in reducing pain, improving functionality, joint range of motion (ROM), quality of life, fatigue, and health status self-perception in people with MPS.

Methods
PubMed, the Cochrane Library, CINAHL, the Physiotherapy Evidence Database (PEDro), and SPORTDiscus were systematically searched for only randomized clinical trials published up to June 2, 2022. The main outcome variables were pain, as reported on the visual analog scale (VAS) and pressure pain threshold (PPT), and functionality. A quantitative analysis was conducted using the inverse variance method and the random effects model.

Results
Twenty-seven studies were included (n = 595 participants in the ESWT group). The effectiveness of ESWT for relieving pain was superior for the ESWT group compared to the control group on the VAS (MD = −1.7 cm; CI 95%: −2.2 to −1.1) and PPT (MD = 1.1 kg/cm2; CI 95%: 0.4 to 1.7) and functionality (SMD = −0.8; CI 95%: −1.6 to −0.04) with high heterogeneity. However, no differences were found between ESWT and other interventions as dry needling, exercises, infiltrations, and lasers interventions.

Conclusion
ESWT is effective in relieving pain and improving functionality in patients with MPS compared to control and ultrasound therapy.
Effect of Selective Serotonin Reuptake Inhibitors on Motor Recovery After Stroke: A Systematic Meta-analysis
Authors: Oh Ju Sun , Choo Yoo Jin , Chang Min Cheol 
Published: May 2023

Abstract

Objective
We conducted a meta-analysis to determine the effectiveness of SSRIs in improving motor outcomes after stroke. For accuracy, we only included studies in which SSRIs were administered to patients in the recovery phase after stroke (<6 months after stroke).

Design
Meta-analyses were conducted according to the tools used to measure motor function. We searched the SCOPUS, PubMed, Embase, and Cochrane Library databases for studies which compared motor recovery in patients who receive SSRI medication in the recovery phase after stroke with a control group that did not receive any SSRI.

Results
A total of 3,715 publications were assessed, and nine studies met the study criteria. The group which received SSRI showed improved Fugl-Meyer Motor Scale and Barthel index scores compared to the control group. However, there was no significant difference in the modified Rankin Scale scores between the SSRI and control groups. The incidence of adverse effects after the administration of SSRIs did not differ from that in the control group.

Conclusion
Our study showed that the use of SSRI in the recovery phase of stroke improved motor function without significant increase in side effects.
Accuracy of Ultrasound Imaging for Etiology of New-Onset Painless Jaundice
Authors: Rosen Sarah J. , Beier Matthew A. , Parikh Manish , Kim Sooah , Hindman Nicole 
Published: May 2023

Purpose
Ultrasound (US) is considered a first-line study for painless jaundice. However, in our hospital system, patients with new-onset painless jaundice often have a contrast-enhanced computed tomography (CECT) or magnetic resonance cholangiopancreatography (MRCP) regardless of the sonographic findings. Thus, we investigated the accuracy of US for detection of biliary dilatation in patients with new-onset painless jaundice.

Methods
Our electronic medical record was searched from January 1, 2012, to January 1, 2020, for adult patients with new-onset painless jaundice. Presenting complaint/setting, laboratory values, imaging studies/findings, and final diagnoses were recorded. Patients with pain or known liver disease were excluded. A gastrointestinal physician reviewed the laboratory values/chart to classify the type of suspected obstruction. Two radiologists blindly re-reviewed the US scans, and κ between the radiologists was calculated. Fisher exact test and the 2-sample t test were used for statistical analysis.

Results
Three hundred sixty patients presented with jaundice (>3 mg/dL), of whom 68 met the inclusion criteria (no pain and no known liver disease). Laboratory values had an overall accuracy of 54%, but were accurate in 87.5% and 85% for obstructing stones/pancreaticobiliary cancer. Ultrasound demonstrated overall accuracy of 78%, but only 69% for pancreaticobiliary cancer and 12.5% for common bile duct stone. Seventy-five percent of the patients underwent follow-up CECT or MRCP regardless of presenting setting. In the emergency department or inpatient setting, 92% of the patients underwent CECT or MRCP regardless of US, and 81% had follow-up CECT or MRCP within 24 hours.

Conclusion
A US-first strategy in the setting of new-onset painless jaundice is accurate only 78% of the time. In practice, US was almost never a stand-alone imaging examination in patients presenting to the emergency department or inpatient setting with new-onset painless jaundice, no matter the suspected diagnosis based on clinical and laboratory grounds or on the US findings themselves. However, for milder elevations of unconjugated bilirubin (suspicious for Gilbert disease) in the outpatient setting, a US demonstrating lack of biliary dilatation was often a definitive study for exclusion of pathology.
What Is New in Stage 3 of the 2019 Revised Association Research Circulation Osseous Staging System of Osteonecrosis of the Femoral Head: A Relationship to Bone Resorption
Authors: Bae Jiwon , Lee Seul Ki , Kim Jiyoung , Kim Jee-Young , Kim Jun-Ho 
Published: May 2023

Purpose
The aim of the study is to evaluate the stage 3 findings of the 2019 revision of the Association Research Circulation Osseous (ARCO) staging system for osteonecrosis of the femoral head between 3A and 3B and the relationship with bone resorption area.

Materials and Methods
We retrospectively enrolled 87 patients with ARCO stage 3 osteonecrosis of the femoral head, divided into stage 3A (n = 73) and 3B (n = 14). The revised stage 3 findings included subchondral fracture, fracture in necrotic portion, and flattening of the femoral head and were compared between stage 3A and 3B. The association between these findings and the causative features of bone resorption area was also evaluated.

Results
All stage 3 cases had subchondral fractures. In stage 3A, these fractures were generated by crescent sign (41.1%) and by fibrovascular reparative zone in 58.9%; however, in stage 3B, fibrovascular reparative zone generated 92.9% of these fractures and crescent sign only 7.1% with statistical significance (P = 0.034). Necrotic portion fracture was noted in 36.7% and femoral head flattening was observed in 14.9% of all stage 3. Necrotic portion fracture (92.9% vs 26.0%) and femoral head flattening (71.4% vs 4.1%) were observed more frequently in stage 3B than 3A (P < 0.001). Almost all subchondral fractures by fibrovascular reparative zone (96.4%) and necrotic portion fracture (96.9%), and all femoral head flattening was presented with bone resorption area with expanding areas.

Conclusions
The ARCO stage 3 descriptions reflect severity in this order: subchondral fracture, necrotic portion fracture, and femoral head flattening. More severe findings are usually associated with expanding bone resorption areas.
Robotic versus Laparoscopic Ventral Hernia Repair
Authors: Dhanani Naila H , Lyons Nicole B , Olavarria Oscar A , Bernardi Karla , Holihan Julie L , Shah Shinil K , Wilson Todd D , Loor Michele M , Kao Lillian S , Liang Mike K 
Published: May 2023

Objective:
Report the two-year outcomes of a multi-center randomized controlled trial comparing robotic versus laparoscopic intraperitoneal onlay mesh ventral hernia repair.

Summary Background Data:
Ventral hernia repair is one of the most common operations performed by general surgeons. To our knowledge, no studies have been published to date comparing long-term outcomes of laparoscopic versus robotic ventral hernia repair.

Methods:
The trial was registered at clinicaltrials.gov (NCT03490266). Clinical outcomes included surgical site infection, surgical site occurrence, hernia occurrence, readmission, reoperation, and mortality.

Results:
175 consecutive patients were approached that were deemed eligible for elective minimally invasive ventral hernia repair. 124 were randomized and 101 completed follow-up at two years. Two-year follow-up was completed in fifty-four patients (83%) in the robotic arm and 47 patients (80%) in the laparoscopic arm. No differences were seen in surgical site infection or surgical site occurrence. Hernia recurrence occurred in two patients (4%) receiving robotic repair versus in six patients (13%) receiving laparoscopic repair (relative risk 0.3, 95% confidence interval 0.06 to 1.39; P=0.12). No patients (0%) required reoperation in the robotic arm whereas five patients (11%) underwent reoperation in the laparoscopic arm (P=0.019, relative risk not calculatable due to null outcome).

Conclusions:
Robotic ventral hernia repair demonstrated at least similar if not improved outcomes at two-years compared to laparoscopy. There is potential benefit with robotic repair, however additional multi-center trials and longer follow-up are needed to validate the hypothesis-generating findings of this study.
Functional insight and cell-specific expression of the adipokinetic hormone/corazonin-related peptide in the human disease vector mosquito,Aedes aegypti
Authors: Afifi Salwa , Wahedi Azizia , Paluzzi Jean-Paul 
Published: May 2023

AbstractThe adipokinetic hormone/corazonin-related peptide (ACP) is an insect neuropeptide structurally intermediate between corazonin (CRZ) and adipokinetic hormone (AKH). Unlike the AKH and CRZ signaling systems that are widely known for their roles in the mobilization of energy substrates and stress responses, respectively, the main role of ACP and its receptor (ACPR) remains unclear in most arthropods. The current study aimed to localize the distribution of ACP in the nervous system and provide insight into its physiological roles in the disease vector mosquito,Aedes aegypti. Immunohistochemical analysis and fluorescencein situhybridization localized the ACP peptide and transcript within a number of cells in the central nervous system, including two pairs of laterally positioned neurons in the protocerebrum of the brain and a few ventrally localized neurons within the pro- and mesothoracic regions of the fused thoracic ganglia. Further, extensive ACP-immunoreactive axonal projections with prominent blebs and varicosities were observed traversing the abdominal ganglia. Given the prominent enrichment ofACPRexpression within the abdominal ganglia of adultA. aegyptimosquitoes as determined previously, the current results indicate that ACP may function as a neurotransmitter and/or neuromodulator facilitating communication between the brain and posterior regions of the nervous system. In an effort to elucidate a functional role for ACP signaling, biochemical measurement of energy nutrients in female mosquitoes revealed a reduction in abdominal glycogen stores in response to ACP that matched the actions of AKH, but interestingly, a corresponding hypertrehalosaemic effect was only found in response to AKH since ACP did not influence circulating carbohydrate levels. Comparatively, both ACP and AKH led to a significant increase in haemolymph carbohydrate levels in male mosquitoes while both peptides had no influence on their glycogen stores. Neither ACP nor AKH influenced circulating or stored lipid levels in both male and female mosquitoes. Collectively, these results reveal ACP signaling in mosquitoes may have complex sex-specific actions, and future research should aim to expand knowledge on the role of this understudied neuropeptide.
Plastid anionic lipids are required for membrane development and protochlorophyllide synthesis in etioplasts
Authors: Yoshihara Akiko , Kobayashi Keiko , Nagata Noriko , Fujii Sho , Wada Hajime , Kobayashi Koichi 
Published: May 2023

ABSTRACTDark-germinated angiosperms develop the chloroplast precursors called etioplasts in cotyledon cells. Etioplasts develop lattice membrane structures called prolamellar bodies (PLBs), where the chlorophyll intermediate protochlorophyllide (Pchlide) forms a ternary complex with NADPH and light-dependent NADPH-Pchlide oxidoreductase (LPOR). The lipid bilayers of etioplast membranes are mainly composed of galactolipids, which play important roles in membrane-associated processes in etioplasts. Although etioplast membranes also contain two anionic lipids, phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), the roles of these anionic lipids are unknown. To reveal the importance of PG and SQDG for the development of etioplasts, we characterized etiolated Arabidopsis mutants deficient in the biosynthesis of PG and SQDG. A partial deficiency in PG biosynthesis loosened the lattice structure of PLBs and impaired the insertion of Mg2+into protoporphyrin IX, leading to a significant decrease in Pchlide content. Although a complete lack of SQDG biosynthesis did not notably affect both PLB formation and Pchlide biosynthesis, the lack of SQDG in addition to the partial deficiency of PG caused strong impairments of these processes. The results suggested that PG is required for PLB formation and Pchlide biosynthesis, whereas SQDG plays an auxiliary role in these processes. Notably, the PG deficiency and the lack of SQDG oppositely affected the dynamics of LPOR complexes after photoconversion, suggesting different involvements of PG and SQDG in the organization of LPOR complexes. Our data demonstrate pleiotropic roles of anionic lipids in etioplast development.
Explicit Ion Modeling Predicts Physicochemical Interactions for Chromatin Organization
Authors: Lin Xingcheng , Zhang Bin 
Published: May 2023

AbstractMolecular mechanisms that dictate chromatin organizationin vivoare under active investigation, and the extent to which intrinsic interactions contribute to this process remains debatable. A central quantity for evaluating their contribution is the strength of nucleosome-nucleosome binding, which previous experiments have estimated to range from 2 to 14kBT. We introduce an explicit ion model to dramatically enhance the accuracy of residue-level coarse-grained modeling approaches across a wide range of ionic concentrations. This model allows forde novopredictions of chromatin organization and remains computationally efficient, enabling large-scale conformational sampling for free energy calculations. It reproduces the energetics of protein-DNA binding and unwinding of single nucleosomal DNA, and resolves the differential impact of mono and divalent ions on chromatin conformations. Moreover, we showed that the model can reconcile various experiments on quantifying nucleosomal interactions, providing an explanation for the large discrepancy between existing estimations. We predict the interaction strength at physiological conditions to be 9kBT, a value that is nonetheless sensitive to DNA linker length and the presence of linker histones. Our study strongly supports the contribution of physicochemical interactions to the phase behavior of chromatin aggregates and chromatin organization inside the nucleus.
Percutaneous coronary intervention with drug-eluting stent is associated with better survival than coronary artery bypass grafting in Taiwan dialysis patients
Authors: Pan Szu-Yu , Yang Ju-Yeh , Teng Nai-Chi , Chen Yun-Yi , Wang Shi-Heng , Lee Chien-Lin , Chen Kang-Lung , Chiu Yen-Ling , Hsu Shih-Ping , Peng Yu-Sen , Chen Yung-Ming , Lin Shuei-Liong , Chen Likwang 
Published: May 2023

AbstractRationale and ObjectiveTo study the comparative effectiveness of percutaneous coronary intervention with drug-eluting stent and coronary artery bypass grafting in dialysis patients.Study DesignRetrospective observational cohort study.Setting and ParticipantsThis population-based study identified dialysis patients hospitalized for coronary revascularization between January 1, 2009 and December 31, 2015 in Taiwan National Health Insurance Research Database.ExposuresPercutaneous coronary intervention with drug-eluting stent versus coronary artery bypass grafting.OutcomesAll-cause mortality, in-hospital mortality, and repeat revascularization.Analytical ApproachPropensity scores were used to match patients. Cox proportional hazards models and logistic regression models were constructed to examine associations between revascularization strategies and mortality. Interval Cox models were fit to estimate time-varying hazards during different periods.ResultsA total of 1,840 propensity score-matched dialysis patients were analyzed. Coronary artery bypass grafting was associated with higher in-hospital mortality (coronary artery bypass grafting vs. percutaneous coronary intervention with drug-eluting stent, crude mortality rate 12.5% vs. 3.3%; adjusted odds ratio 5.22; 95% confidence interval [CI] 3.42-7.97; p < 0.001), and longer hospitalization duration (median [interquartile range], 20 [14-30] days vs. 3 [2-8] days, p < 0.001). After discharge, repeat revascularization, acute coronary syndrome, and repeat hospitalization all occurred more frequently in the percutaneous coronary intervention with drug-eluting stent group. Importantly, with a median follow-up of 2.8 years, coronary artery bypass grafting was significantly associated with a higher risk of all-cause overall mortality (adjusted hazard ratio 1.19, 95% CI 1.05-1.35, p < 0.01) in the multivariable Cox proportional hazard model. Sensitivity and subgroup analyses yielded consistent results.LimitationsObservational study with mainly Asian ethnicity.ConclusionsPercutaneous coronary intervention with drug-eluting stent may be associated with better survival than coronary artery bypass grafting in dialysis patients. Future studies are warranted to confirm this finding.
Model Parameter identification using 2D vs 3D experimental data: a comparative analysis
Authors: Cortesi Marilisa , Liu Dongli , Yee Christine , Marsh Deborah J. , Ford Caroline E. 
Published: May 2023

AbstractComputational models are becoming an increasingly valuable tool in biomedical research. They enable the quantification of variables difficult to measure experimentally, an increase in the spatio-temporal resolution of the experiments and the testing of hypotheses.Parameter estimation fromin-vitrodata, remains a challenge, due to the limited availability of experimental datasets acquired in directly comparable conditions. While the use of computational models to supplement laboratory results contributes to this issue, a more extensive analysis of the effect of incomplete or inaccurate data on the parameter optimization process and its results is warranted. To this end, we compared the results obtained from the samein-silicomodel of ovarian cancer cell growth and metastasis, calibrated with datasets acquired from two different experimental settings: a traditional 2D monolayer, and 3D cell culture models.The differential behaviour of these models will inform the role and importance of experimental data in the calibration of computational models’ calibration. This work will also provide a set of general guidelines for the comparative testing and selection of experimental models and protocols to be used for parameter optimization in computational modelsAuthor summaryParameter identification is a key step in the development of a computational model, that is used to establish a connection between the simulated and experimental results and verify the accuracy of thein-silicoframework.The selection of thein-vitrodata to be used in this phase is fundamental, but little attention has been paid to the role of the experimental model in this process. To bridge this gap we present a comparative analysis of the same computational model calibrated using experimental data acquired from cells cultured (i) in 2D monolayers, (ii) in 3D culture models and (iii) a combination of the two.Data acquired in different experimental settings induce changes in the optimal parameter sets and the corresponding computational model’s behaviour. This translates in a varying degree of accuracy during the validation procedure, when the simulated data are compared to experimental measurements not used during the calibration step.Overall, our work provides a workflow and a set of guidelines to select the most appropriate experimental setting for the calibration and validation of computational models.
A Recombinant Antibody For Tracking Murine Gammaherpesvirus 68 Uracil DNA Glycosylase Expression
Authors: Mu Yunxiang , Plummer Joshua B. , Zelazowska Monika A. , Paul Somnath , Dong Qiwen , Chen Zaowen , Krug Laurie T. , McBride Kevin M. 
Published: May 2023

AbstractAntibodies are powerful tools to detect expressed proteins. However off-target recognition can confound their use. Therefore, careful characterization is needed to validate specificity in distinct applications. Here we report the sequence and characterization of a mouse recombinant antibody that specifically detects ORF46 of murine gammaherpesvirus 68 (MHV68). This ORF encodes the viral uracil DNA glycosylase (vUNG). The antibody does not recognize murine uracil DNA glycosylase and is useful in detecting vUNG expressed in virally infected cells. It can detect expressed vUNG in cells via immunostaining and microscopy or flow cytometry analysis. The antibody can detect vUNG from lysates of expressing cells via immunoblot under native conditions but not denaturing conditions. This suggests it recognizes a confirmational based epitope. Altogether this manuscript describes the utility of the anti-vUNG antibody and suitability for use in studies of MHV68 infected cells.