bluuebunny/crossref_metadata_2025_split huggingface dataset:
Higher sensitive selective spectrofluorometric determination of ritonavir in the presence of nirmatrelvir: application to new FDA approved co-packaged COVID-19 pharmaceutical dosage and spiked human plasma
Authors: Imam Mohamed S. , Abdelazim Ahmed H. , Ramzy Sherif , Almrasy Ahmed A. , Gamal Mohammed , Batubara Afnan S. 
Published: September 2023

Abstract
Background
Ritonavir was recently combined with nirmatrelvir in a new approved co-packaged medication form for the treatment of COVID-19. Quantitative analysis based on fluorescence spectroscopy measurement was extensively used for sensitive determination of compounds exhibited unique fluorescence features.

Objective
The main objective of this work was to develop higher sensitive cost effective spectrofluorometric method for selective determination of ritonavir in the presence of nirmatrelvir in pure form, pharmaceutical tablet as well as in spiked human plasma.

Methods
Ritonavir was found to exhibit unique native emission fluorescence at 404 nm when excited at 326 nm. On the other hand, nirmatrelvir had no emission bands when excited at 326 nm. This feature allowed selective determination of ritonavir without any interference from nirmatrelvir. The variables affecting fluorescence intensity of ritonavir were optimized in terms of sensitivity parameters and principles of green analytical chemistry. Ethanol was used a green solvent which provided efficient fluorescence intensity of the cited drug.

Results
The method was validated in accordance with the ICH Q2 (R1) standards in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision and specificity. The described method was successfully applied for ritonavir assay over the concentration range of 2.0–20.0 ng/mL.

Conclusion
Ritonavir determination in the spiked human plasma was successfully done with satisfactory accepted results.
An exploratory study on the possibilities of microalgal biotechnology to obtain the essential 6Li isotope as fusion fuel
Authors: García-Balboa Camino , Martínez-Alesón Paloma , López-Rodas Victoria , Costas Eduardo Costas , Díaz Marta Fernández 
Published: September 2023

AbstractFuture energy supply needs to overcome two challenges: environmental impact and dependence on geopolitically unstable countries. A very promising alternative is based on lithium, an element for batteries, and whose isotope 6Li will be essential in nuclear fusion. The objective of this research has been to determine if it is possible to achieve isotopic fractionation of lithium through a process mediated by microalgae. For this purpose, Chlamydomonas reinhardtii was selected and grown in presence of 5 mg/L of lithium. Results revealed that this specie survives at the selected lithium concentration, discriminates isotopes and preferentially capture 6Li (6δ = 10.029 ± 3.307) through a process independent of the cellular growth. Concomitate recovered up 0.206 mg/L of lithium along a process of 21 days. The result of this study lets to affirm that Chlamydomonas reinhardtii might be used to obtain lithium enriched in the lighter isotope.
Influence of the timing of biological treatment initiation on Juvenile Idiopathic Arthritis long-term outcomes
Authors: Oliveira Ramos Filipa , Rodrigues Ana Maria , Melo Ana Teresa , Aguiar Francisca , Brites Luísa , Azevedo Soraia , Duarte Ana Catarina , Gomes José António Melo , Furtado Carolina , Mourão Ana Filipa , Sequeira Graça , Cunha Inês , Figueira Ricardo , Santos Maria José , Fonseca João Eurico 
Published: September 2023

Abstract
Background
Juvenile idiopathic arthritis (JIA) treatment is aimed at inducing remission to prevent joint destruction and disability. However, it is unclear what is the long-term impact on health-related outcomes of the timing of biological disease-modifying antirheumatic drug (bDMARD) initiation in JIA. Our aim was to evaluate the long-term impact of the time between JIA onset and the initiation of a bDMARD in achieving clinical remission, on physical disability and health-related quality of life (HRQoL).

Methods
Adult JIA patients registered in the Rheumatic Diseases Portuguese Register (Reuma.pt) and ever treated with bDMARD were included. Data regarding socio-demographic, JIA-related characteristics, disease activity, physical disability (HAQ-DI), HRQoL (SF-36), and treatments were collected at the last visit. Patients were divided into 3 groups (≤ 2 years, 2–5 years, or > 5 years), according to the time from disease onset to bDMARD initiation. Regression models were obtained considering remission on/off medication, HAQ-DI, SF-36, and joint surgeries as outcomes and time from disease onset to bDMARD start as an independent variable.

Results
Three hundred sixty-one adult JIA patients were evaluated, with a median disease duration of 20.3 years (IQR 12.1; 30.2). 40.4% had active disease, 35.1% were in remission on medication, and 24.4% were in drug-free remission; 71% reported some degree of physical disability. Starting a bDMARD > 5 years after disease onset decreased the chance of achieving remission off medication (OR 0.24; 95% CI 0.06, 0.92; p = 0.038). Patients who started a bDMARD after 5 years of disease onset had a higher HAQ and worse scores in the physical component, vitality, and social function domains of SF-36, and more joint surgeries when compared to an earlier start.

Conclusion
Later initiation of bDMARDs in JIA is associated with a greater physical disability, worse HRQoL, and lower chance of drug-free remission in adulthood.
Human umbilical cord-derived mesenchymal stem cells ameliorate perioperative neurocognitive disorder by inhibiting inflammatory responses and activating BDNF/TrkB/CREB signaling pathway in aged mice
Authors: Wei Penghui , Jia Min , Kong Xiangyi , Lyu Wenyuan , Feng Hao , Sun Xinyi , Li Jianjun , Yang Jian-jun 
Published: September 2023

Abstract
Background
Perioperative neurocognitive disorder (PND) is a key complication affecting older individuals after anesthesia and surgery. Failure to translate multiple pharmacological therapies for PND from preclinical studies to clinical settings has necessitated the exploration of novel therapeutic strategies. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) treatment has emerged as a promising therapeutic strategy for treating neurodegenerative diseases and has the potential to translate basic science into clinical practice. In this study, we investigated the effects and underlying mechanism of hUC-MSCs on PND in aged mice.

Methods
hUC-MSCs were isolated from an infant umbilical cord and identified using flow cytometry and differentiation assays. We established PND model by undergoing aseptic laparotomy under isoflurane anesthesia maintaining spontaneous ventilation in eighteen-month-old male C57BL/6 mice. hUC-MSCs were slowly injected into mice by coccygeal vein before anesthesia. Cognitive function, systemic and neuroinflammatory responses, neuroplasticity, endogenous neurogenesis, and brain-derived neurotrophic factor (BDNF) were assessed. To determine the brain mechanisms underlying by which hUC-MSCs mediate their neuroprotective effects in PND, K252a, an antagonist of BDNF receptor, was administered intraperitoneally before surgery. Hippocampal BDNF/TrkB/CREB signaling pathway and metabolomic signatures were evaluated.

Results
hUC-MSC treatment ameliorated the learning and memory impairment in aged mice with PND. The downstream effects were the suppression of systemic and hippocampal inflammation and restoration of neurogenesis and neuroplasticity dysregulation. Interestingly, the level of mature BDNF, but not that of proBDNF, was increased in the hippocampus after hUC-MSC treatment. Further analysis revealed that the improved cognitive recovery and the restoration of neurogenesis and neuroplasticity dysregulation elicited by exposure to hUC-MSCs were, at least partially, mediated by the activation of the BDNF/TrkB/CREB signaling pathway. Untargeted metabolomic further identified lipid metabolism dysfunction as potential downstream of the BDNF/TrkB/CREB signaling pathway in hUC-MSC-mediated neuroprotection for PND.

Conclusions
Our study highlights the beneficial effects of hUC-MSC treatment on PND and provides a justification to consider the potential use of hUC-MSCs in the perioperative period.
Effects of Larrea nitida nanodispersions on the growth inhibition of phytopathogens
Authors: Rocha Felipe , Nunes Calumby Rodrigo José , Svetaz Laura , Sortino Maximiliano , Teixeira Ribeiro Vidigal Márcia Cristina , Campos-Bermudez Valeria Alina , Rius Sebastián Pablo 
Published: September 2023

AbstractLarrea nitida Cav. (Zygophyllaceae) is a plant endemic to Argentina and Chile, and its extract has been studied over the last years due to the presence of antimicrobial agents that can be used to control the growth of some pathogens in agriculture. However, the extract is highly hydrophobic, which strongly affects its fungicidal activity in aqueous media. In this sense, the solid dispersion technique was used to produce L. nitida extract nanodispersions with polyethylene glycol (PLE) and with polyethylene glycol and zinc acetate (PZLE). In order to further evaluate the activity of the extract in PLE and PZLE, blank nanodispersions containing only polyethylene glycol (PEG) and zinc acetate (PZ) without the addition of the extract were also produced. The fungicidal activity of the water-soluble nanoparticles was evaluated at different concentrations (0.037–0.110 g.mL−1). In general, the nanoparticles were successfully produced on a nanometric size and presented a significant inhibitory activity on the growth of the pathogens Fusarium oxysporum and Fusarium verticillioides in aqueous media. Compared to PLE, PZLE presented increased fungistatic activity, possibly due to their increased solubility in water. Even though their application in agriculture should be further investigated, the nanodispersions present great potential to be applied as a green biotechnological tool.
I want to break free: how a design approach guides nascent intrapreneurs in a global public service organisation
Authors: van den Broek Antonius , Koria Mikko , Saarelainen Emilia 
Published: September 2023

AbstractIn this paper, we expand our understanding of how a design approach can enable and guide employees to break free from their incumbent mindset and ways of working and become intrapreneurs in public organisations. While previous studies have identified desired behaviour for intrapreneurs, there is a lack of understanding how new intrapreneurs adapt such behaviours, especially in public organisations. This paper shows how the readily available approaches of design practice can guide new intrapreneurs to break free from their routines, adopt entrepreneurial behaviours and provide the practical tools to help them to persuade others to collaborate with them on their innovative ideas. We draw from 19 interviews with nascent intrapreneurs, and documentation collected at the UN Refugee Agency to inductively develop a model for how design supports employees in their journey to become an intrapreneur. Our findings illustrate how adopting a design approach provided aspiring intrapreneurs to become ambidextrous in navigating between the creative and the calculative logics and heuristics in highly structured organisations. Both the design attitude and the tools associated with design practice helped new intrapreneurs to adopt an entrepreneurial mindset, position their new ideas and align them to the needs, priorities and constraints of colleagues and stakeholders.
Global trends and hotspots in research on acupuncture for stroke: a bibliometric and visualization analysis
Authors: Zhang Jiale , Ji Chenyang , Zhai Xu , Ren Shuo , Tong Hongxuan 
Published: September 2023

AbstractAcupuncture has been widely used in stroke and post-stroke rehabilitation (PSR), but there is no literature on the bibliometric analysis of acupuncture for stroke. This study aimed to characterize the global publications and analyze the trends of acupuncture for stroke in the past 40 years. We identified 1157 publications from the Web of Science Core Collection. The number of publications grew slowly in the first three decades from 1980 until it started to grow after 2010, with significant growth in 2011–2012 and 2019–2020. China, the USA, and South Korea are the top three countries in this field, and China has formed good internal cooperative relations. Early studies focused on the clinical efficacy of acupuncture for stroke. In the last five years, more emphasis has been placed on the effectiveness of acupuncture in treating sequelae and complications, combined with neuroimaging studies to explore the mechanisms of brain injury repair and neurological recovery. Acupuncture for stroke has a vast research potential, and researchers from different countries/regions and organizations still need to remove academic barriers to enhance communication and collaboration.
The global prevalence of suicidal ideation and suicide attempts among men who have sex with men: a systematic review and meta-analysis
Authors: Nouri Elham , Moradi Yousef , Moradi Ghobad 
Published: September 2023

Abstract
Background
This study aimed to determine the global prevalence of suicidal ideation and attempts among men who have sex with men (MSM) as a systematic review, and meta-analysis.

Methods
For this meta-analysis, a search in four international databases (PubMed, Scopus, Web of Science, and EMBASE) was designed, and performed. In the next step, the information extraction checklist was prepared based on the study authors’ opinions, and the quality of the articles was evaluated using the Newcastle–Ottawa scale (NOS) checklist. Data meta-analysis was performed using STATA16 software with a significance level below 0.05.

Results
The results showed the prevalence of suicidal ideation, and suicide attempts among MSM was 21% (95% CI 17%-26%), and 12% (95% CI 8%-17%), respectively. The results of the subgroup analysis showed that the prevalence of suicidal ideation in the population of MSM living with Human immunodeficiency virus (HIV) was 40% (95% CI 35%–45%), and the prevalence of suicide attempts among MSM with HIV was 10% (95% CI 1%–27%). The prevalence of suicidal ideation in European MSM, and the prevalence of suicide attempts among American MSM were higher than other MSM in other geographical areas.

Conclusion
Considering that the prevalence of suicidal ideation and attempts among these people is many times higher than that among men in the general population, developing programs for the prevention of mental disorders with special attention to suicide is necessary for these people. Screening programs are also recommended for early diagnosis and prevention of suicide among these people.
Supporting agriculture in developing countries: new insights on the impact of official development assistance using a climate perspective
Authors: Trentinaglia Maria Teresa , Baldi Lucia , Peri Massimo 
Published: September 2023

AbstractAgriculture is a major source of food and income for poor and rural households living in developing countries; yet, agricultural systems are increasingly threatened by changing climate conditions that compromise their productivity and resilience. Over time, international aid has provided support to the agricultural systems of recipient countries, though the literature is not unanimous in confirming their effectiveness.To shed light on this issue, the purpose of this work is to assess the efficacy of these aid in increasing the agricultural productivity of recipient nations, employing original approaches.First, to adopt a climate change perspective, we conduct our analysis using a recent classification adopted by the Official Development Assistance—the Rio Markers—which distinguishes aid between adaptation and mitigation to climate change.Second, taking into account that the starting conditions of recipient countries can significantly impact aid effectiveness, we classify 115 developing countries into four subgroups according to their vulnerability and readiness to climate change, as evaluated by the ND-Gain indicators.We perform a two-stage instrumental variable approach within the context of panel models to investigate the potential growth-enhancing impact that different types of agricultural aid may exert on the agriculture Total Factor Productivity in recipient countries.Our findings show that aid to agriculture, especially adaptation aid, has a positive impact on agricultural productivity growth. We also observe that countries with a higher climate readiness benefit the most from aid, whereas countries highly vulnerable and heavily dependent on the agricultural sector are less able to leverage the aid received to the same extent.Overall, our analysis confirms the importance of international aid to the agricultural sector and suggests that accurate impact assessment analyses should also consider a climate perspective to distinguish adaptation from mitigation aid.
ANXA1 is identified as a key gene associated with high risk and T cell infiltration in primary sclerosing cholangitis
Authors: Zhang Jian , Wang Huiwen , Liu Jinqing , Fu Lei , Peng Shifang 
Published: September 2023

Abstract
Background
Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease, with unclear pathogenesis. Although immune disorders, especially T cell infiltration, are thought to play a vital role in PSC, the specific pathogenesis mechanisms remain incompletely understood. This study evaluated the potential key gene associated with the PSC pathogenesis and analyzed the associations of the key gene with prognosis and immune cell infiltration by combining bioinformatics analysis and experimental verification.

Methods
Transcriptome data of PSC and normal human liver tissues (GSE159676) were obtained from the gene expression omnibus database. Differentially expressed genes (DEGs) were identified, and differences in biological states were analyzed. A protein–protein interaction (PPI) network was constructed. Hub genes were identified, and their expression was verified using transcriptome data of mice fed 0.1% 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) and Mdr2−/− mice (GSE179993, GSE80776), as well as by immunohistochemistry staining on clinical samples. The correlations between the key gene and other factors were evaluated by Pearson’s correlation coefficient. Immune cell infiltration into human liver (GSE159676) was analyzed by xCell and verified by immunofluorescence staining on PSC liver samples.

Results
Of the 185 DEGs identified, 113 were upregulated and 72 were downregulated genes in PSC. Genes associated with immune cell infiltration and fibrosis were significantly enriched in PSC. PPI network showed close interactions among DEGs. A module strongly associated with immune infiltration was identified, with annexin A1 (ANXA1) being the core gene. High expression of ANXA1 in PSC was confirmed in two public datasets and by immunohistochemistry staining on clinical samples. High ANXA1 expression was strongly associated with high-risk score for PSC. Also, ANXA1 expression was positively associated with chemokines and chemokine receptors and with the infiltration of immune cells, especially T cells, into liver with PSC. Immune infiltration, fibrosis, and cancer-related processes were markedly enriched in PSC with high expression of ANXA1.

Conclusion
ANXA1 is a key gene associated with high risk and infiltration of immune cells, especially T cells, in PSC. These findings provide new insight into the key biomarker of PSC and suggest that targeting ANXA1 may be a valuable strategy for the treatment of PSC.
Diurnal migration patterns in willow warblers differ between the western and eastern flyways
Authors: Sokolovskis Kristaps , Caballero-Lopez Violeta , Åkesson Susanne , Lundberg Max , Willemoes Mikkel , Zhao Tianhao , Bensch Staffan 
Published: September 2023

AbstractIt is a long-standing view that the main mechanism maintaining narrow migratory divides in passerines is the selection against intermediate and suboptimal migratory direction, but empirical proof of this is still lacking. We present novel results from a willow warbler migratory divide in central Sweden from where birds take the typical SW and SE as well as intermediate routes to winter quarters in Africa. We hypothesized that individuals that take the intermediate route are forced to migrate in daytime more often when crossing wide ecological barriers than birds that follow the typical western or eastern flyways. Analyses of geolocator tracks of willow warblers breeding across the entire Sweden, including the migratory divide, provided no support for our hypothesis. Instead, birds that migrated along the western flyway were the most likely to undertake full day flights. The probability of migrating for a full day when crossing major barriers declined linearly from west to east. We speculate that this difference is possibly caused by more challenging conditions in the western part of the Sahara Desert, such as the lack of suitable day-time roost sites. However, it may equally likely be that willow warblers benefit from migrating in daytime if favorable tailwinds offer assistance.
From South Asia to Southern Europe: a comparative analysis of Sri Lankans’ residential segregation in the main Italian cities using high-resolution data on regular lattice geographies
Authors: Bitonti Francesca , Benassi Federico , Mazza Angelo , Strozza Salvatore 
Published: September 2023

AbstractThe present work proposes a spatial analysis of the residential segregation and settlement models of Sri Lankans in the eight main Italian municipalities. Hosting more than half of the total Sri Lankan population residing in Italy, the selected urban areas allow Sri Lankans’ residential model to be globally framed across the country. The purpose of this work is threefold. First, it provides a general assessment of the allocation pattern of a foreign community that has seldom been studied and yet is characterized by peculiar settlement choices. Second, it attempts to compare the settlement patterns of Sri Lankans across different urban contexts. Third, it aims to detect the possible spatial polarization of Sri Lankans in specific neighbourhoods and to verify its spatial correlation with other key variables that constitute proxies of urban neighbourhoods’ socioeconomic inequalities. The study runs multiple aspatial linear models to assess the global variation in concentrations of Sri Lankans related to several socioeconomic predictors. Furthermore, it implements geographically weighted regressions to explicitly model the spatial dependence between Sri Lankans’ location quotients and several predictors. It refers all the considered variables to a single geographic reference grid, enabling the homogenization of different areal unit arrangements and comparisons across space. Except for Milan and Rome, the findings suggest that Sri Lankans tend to reside in central neighbourhoods characterized by a high foreign presence and a decreased trend of Italian population. Conversely, the impact of the cost of living and the state of the built environment is heterogeneous across space, with a sort of centre-periphery duality in Southern cities and more fragmented situations in the other urban contexts. This evidence proves the relevance of local scale analysis and the need to build up urban observatories on spatial inequalities and segregation processes.