bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of potential phosphate solubilizing bacteria inoculants should consider the contrast in phosphorus bio-solubilization rate along with plant growth promotion and phosphorus use efficiency
Authors: Elhaissoufi Wissal , Ibnyasser Ammar , Haddine Meryem , Zeroual Youssef , Ghani Rachid , Barakat Abdellatif , Rchiad Zineb , Ghoulam Cherki , Bargaz Adnane 
Published: March 2023

AbstractAimsAlthough phosphate solubilizing bacteria (PSB) have been globally reported to improve soil phosphorus (P) availability and plant growth, technical gaps such as the lack of an ideal screening approach, is yet to be addressed. The potential of non-halo-forming PSB remains underestimated because of the currently adopted screening protocols that exclusively consider halo-forming and PSB with high phosphorus solubilization (PS) capacities. Yet, caution should be taken to properly assess PSB with contrasting PS rates regardless of the presence or absence of the solubilization halo.Methods and ResultsThis study sought to examine the PS rate and plant growth promotion ability of 12 PSB categorized as high PSB (H-PSB), medium PSB (M-PSB), and low PSB (L-PSB) based on their PS rates of rock phosphate (RP). The non-halo-forming PSB Arthrobacter pascens was categorized as H-PSB, which might have been eliminated during the classical screening process. In addition, induction of organic acids and phosphatase activity in rhizosphere soils by H-, M-, and L-PSB was proportional to increased wheat P content by 143.22, 154.21, and 77.76 mg P g−1 compared to uninoculated plants (18.1 mg P g−1).ConclusionsIsolates considered as M- and L-PSB could positively influence wheat above-ground physiology and root traits as high as H-PSB. In addition, non-halo-forming PSB revealed significant PS rates along with positive effects on plant growth as high as halo-forming PSB.
Brazilian red propolis in combination with β-lactams exerts an efficient antibacterial action over methicillin-resistantStaphylococcus aureus(MRSA) strains
Authors: Ripari Nicolas , Pereira Ana Flávia Marques , Júnior Ary Fernandes , Rall Vera Lúcia Mores , Aldana-Mejía Jennyfer A , Bastos Jairo Kenupp , Sforcin José Maurício 
Published: March 2023

AbstractAimsThe antibacterial activity of red propolis extract (RPE) and brown propolis extracts (BPE) and the synergistic effect of RPE with cefoxitin (CEFO), imipenem (IMI), and ertapenem (ERTA) was evaluated in vitro against methicillin-resistant Staphylococcus aureus (MRSA) strains.Methods and resultsMRSA ATCC 33591, community-associated (CA-MRSA) USA300, and four clinical isolates were used. A broth microdilution assay was performed to obtain inhibitory and bactericidal concentrations of BPE, RPE, CEFO, IMI, and ERTA. RPE in combination with CEFO, IMI, and ERTA was evaluated on the formation or eradication of biofilm. The bacterial relative membrane conductivity of the strains was assessed after RPE and combinations exposition. Surface/binding computational analyzes between RPE compounds and penicillin binding protein 2a (PBP2a) were performed. BPE samples had no activity against MRSA (MICs 3.2–5 g l−1; MBCs 10–15 g l−1), so the subsequent assays were carried out only with RPE and antimicrobials. RPE exerted a bacteriostatic action (MICs 0.0156–0.125 g l−1; MBCs 0.5–2 g l−1) but the combinations with IMI and ERTA showed the highest inhibition, as observed in the time-kill curve. However, the FICI index showed synergism (≥0.5) only to RPE + IMI. This combination was the most effective in inhibiting the biofilm and showed the highest values of membrane conductivity. Computational predictions indicated that RPE constituents may interact with PBP2a.ConclusionRPE and RPE + IMI exerted an antibacterial and antibiofilm activity on MRSA strains probably due to membrane/wall damage and interactions with PBP2a.
De novogenomic analysis ofEnterobacter asburiaeEBRJ12, a plant growth-promoting rhizobacteria isolated from the rhizosphere ofPhaseolus vulgarisL
Authors: Saikia Juthika , Kotoky Rhitu , Debnath Rajal , Kumar Niraj , Gogoi Priyanka , Yadav Archana , Saikia Ratul 
Published: March 2023

AbstractAimEnvironmental stresses such as water deficit induced stress are one of the major limiting factors in crop production. However, some plant growth-promoting rhizobacteria (PGPR) can promote plant growth in such adverse condition. Therefore, the objective was to isolate rhizospheric bacteria from Phaseolus vulgaris L. growing in a drought-affected soil and to analyze its plant growth promoting (PGP) efficacy to black gram (Vigna mungo L.) and Bhut jolokia (Capsicum chinense Jacq.). Whole-genome sequencing of the potential bacteria was targeted to analyze the genetic potential of the isolate as a plant growth-promoting agent.Methods and resultsThe isolate Enterobacter asburiae EBRJ12 was selected based on its PGP efficacy, which significantly improved plant growth and development. The genomic analysis revealed the presence of one circular chromosome of size 4.8 Mb containing 16 genes for osmotic stress regulation including osmotically inducible protein osmY, outer membrane protein A precursor ompA, aquaporin Z, and an operon for osmoprotectant ABC transporter yehZYXW. Moreover, the genome has a complete genetic cluster for biosynthesis of siderophore Enterobactin and siderophore Aerobactin.The PGP effects were verified with black gram and Bhut jolokia in pot experiments. The isolate significantly increased the shoot length by 35.0% and root length by 58.0% of black gram, while 41.0% and 57.0% of elevation in shoot and root length were observed in Bhut jolokia compared to non-inoculated plants.ConclusionsThe EBRJ12 has PGP features that could improve the growth in host plants, and the genomic characterization revealed the presence of genetic potential for plant growth promotion.
Antifungal and antiaflatoxigenic mechanism activity of freeze-dried culture filtrate ofStreptomyces philanthiRL-1-178 on the two aflatoxigenic fungi and identification of its active component
Authors: Boukaew Sawai , Zhang Zhiwei , Prasertsan Poonsuk , Igarashi Yasuhiro 
Published: March 2023

AbstractAimsThe study reports the antifungal and antiaflatoxigenic mechanism activity of freeze-dried culture filtrate of Streptomyces philanthi RL-1-178 (DCF RL-1-178) against two aflatoxigenic strains (Aspergillus parasiticus and A. flavus) and identification of its active component.Methods and ResultsSignificant inhibition in ergosterol biosynthesis by the DCF RL-1-178 appeared on the plasma membrane. Moreover, the DCF RL-1-178 showed dose-dependent inhibition of methylglyoxal (MG) (an aflatoxin inducer) biosynthesis and exhibited a novel antiaflatoxigenic action mechanism. Significant impairments in enzymatic [superoxide dismutase (SOD) and catalase (CAT)] and nonenzymatic [oxidized and reduced glutathione (GSH) and ratio of oxidized and reduced glutathione (GSSG)] anti-oxidative defense molecules were observed in the two aflatoxigenic cells. The active component of the DCF RL-1-178 was identified as natamycin. The natamycin exhibited against A. parasiticus and A. flavus with the minimum inhibitory concentration (MIC) values of 0.5 and 1.0 µg ml−1, respectively, while the minimum fungicidal concentration values were the same (4.0 µg ml−1).ConclusionsThe DCF RL-1-178 containing natamycin exhibited the following effects: (1) inhibition of cellular ergosterol biosynthesis on plasma membrane, (2) reduction in MG (aflatoxin inducer) confirmed novel antiaflatoxigenic mechanism of action, and (3) caused remarkable debasement in antioxidant defense enzymes (SOD and CAT) and nonenzymatic defense molecules (GSH and GSSG) revealing biochemical mechanism of action.
Development and Validation of Stability-Indicating Impurity Profiling Method for Azelastine Hydrochloride and Fluticasone Propionate in Nasal Spray Product Using HPLC with a UV/PDA Detector
Authors: Musmade Bhaskar , Korhale Rasika , Sable Mangal , Lokhande Surbhi , Padmanabhan Sriram , Bhope Shrinivas 
Published: March 2023

Abstract

Background
Azelastine HCl (AZ) and fluticasone propionate (FL) nasal spray drug product is commonly used in the treatment of allergic rhinitis worldwide. To date, the impurity profiling of this product has not been reported.

Objective
The present study aimed to develop and validate a novel RP-HPLC stability-indicating analytical method for the estimation of impurities from AZ and FL nasal spray drug product.

Methods
A mixture of octane sulfonic acid sodium salt and trifluroacetic acid is used as a mobile phase A. Acetonitrile is used as a mobile phase B. Good separation was achieved on Baker bond phenyl hexyl, 250 × 4.6, 5 µm column at 1 mL/min flow rate in gradient elution mode. The chromatograms were monitored at 239 nm.

Results
The LOD and LOQ were found to be 0.006 and 0.019 µg/mL for AZ and 0.010 and 0.030 µg/mL for FL, respectively. The correlation coefficient for all the known impurities and principal analytes was 0.999 from LOQ level to 150% of standard concentration. The recovery for all the known impurities was found to be between 90 and 110%. In the stress study, 15% degradation was observed in basic conditions and 8.7% in acidic conditions. No significant degradation was observed in thermal and oxidative conditions.

Conclusion
An impurity profiling method for AZ and FL combination nasal spray product was successfully developed, validated, and demonstrated to be accurate, precise, specific, robust, and stability-indicating. The method can be routinely used for impurity testing of commercial batches in QC laboratories in the pharmaceutical industry.

Highlights
No impurity study has been reported for this combination product until now.
Development of Method for Evaluation of Edible Bird’s Nest Content in Ready-to-Eat Beverages
Authors: Poon Peggy Miu-Yee , Sze Eric Tung-Po 
Published: March 2023

Abstract

Background
Edible bird’s nest (EBN) is one of the most valuable tonic Chinese foods, made from glutinous salivary secretion with highly concentrated mucin glycoprotein. For ease of consumption, manufacturers have marketed different ready-to-eat EBN products, in which the EBN content varies. This is the first study to analyze the EBN content in ready-to-eat beverages.

Objective
To determine the EBN content in ready-to-eat beverages by its active ingredient, N-acetylneuraminic acid (sialic acid).

Method
Sialic acid in ready-to-eat beverages and raw EBN was extracted in sodium hydrogen sulfate solution, followed by derivatization using o-phenylenediamine dihydrochloride and determination using high-performance liquid chromatography (HPLC). Method precision, recovery of extraction, degradation of sialic acid due to cooking, and measurement uncertainty were evaluated.

Results
The mean concentrations of raw EBN in different origins and colors ranged from 5.77 to 10.92%. Ten different brands of traditional ready-to-eat EBN beverages from the market were analyzed, in which estimated concentrations of EBN were diversified, ranging from 0.014 ± 0.010 to 0.66 ± 0.069% (w/w) (95% confidence level). The concentration of sialic acid was found to range from 11.4 to 527 mg/kg.

Conclusions
Based on the results, sialic acid content can provide a better estimation of the EBN content in traditional ready-to-eat beverages. Neither the selling price nor dried matter could be used as an indicator of the quality of the ready-to-eat EBN beverage among the samples obtained.

Highlights
Sialic acid can be used as an indicator to estimate EBN content, where the sialic acid and EBN content in ready-to-eat beverages from the market were found to vary significantly.
Design of a low-density SNP panel for intramuscular fat content and fatty acid composition of backfat in free-range Iberian pigs
Authors: Palma-Granados Patricia , García-Casco Juan María , Caraballo Carmen , Vázquez-Ortego Pedro , Gómez-Carballar Fernando , Sánchez-Esquiliche Fernando , Óvilo Cristina , Muñoz María 
Published: March 2023

Abstract
Intramuscular fat (IMF) content and fatty acids (FA) profile determine important sensory and technological characteristics of the meat, being interesting traits to be included in breeding programs for Iberian pig. In a previous study, we analyzed the longissimus dorsi transcriptome of Iberian pigs with divergent breeding values for IMF identifying 63 and 12 functional candidate genes and long non-coding RNA being differentially expressed. The first objective of this work was to use the RNA-seq data generated in that study in order to design a low-density panel with 50 single-nucleotide polymorphisms (SNP). The second aim was to evaluate the effects of this SNP panel on IMF content and backfat FA profile in a closed commercial population of Iberian pigs. The IMF content and backfat FA composition were determined in 940 Iberian pigs. A total of 837 SNPs were identified in the selection of genes and lncRNA. Out of the 50 selected SNP, 11 were monomorphic in the population and 2 failed in their genotyping. Association analyses were carried out for the 37 SNP segregating in the population (minor allele frequency, MAF &gt; 0.05). Regarding IMF content, a significant additive effect (q-value &lt; 0.05) was observed for ADIPOQ:g.124646194T &gt; G SNP, in which the G allele was linked to an increase of 7.59% (additive effect expressed as a percentage of the mean of the trait). For the backfat FA composition, we observed 24 SNP with significant associations. The strongest effects were detected for the ELOVL6:g.112186423A &gt; G and FASN_rs331694510G &gt; A SNP. The A allele of both SNPs were associated to a lower percentage of palmitic (C16:0) and palmitoleic (C16:1) acids and an increase in oleic acid (C18:1). In addition, the AMPD3:g.49043886C &gt; G SNP had significant effects on C16:0, stearic (C18:0), C18:1 and on the sum of saturated and monounsaturated fatty acids. Finally, significant effects of the EGR2:66286345G &gt; T SNP on C18:0 and C18:1 and other minor FA were observed. Our findings reveal ADIPOQ:g.124646194T &gt; G, ELOVL6:g.112186423A &gt; G, and FASN_rs331694510G &gt; A may be useful in breeding programs for Iberian pigs including FA profile and IMF content as selection goals, provided that their potential effects on other traits of interest are controlled.
Novel regulatory mechanisms underlying angiogenesis during wound healing revealed by fluorescence-based live-imaging in zebrafish
Authors: Yuge Shinya , Ishii Tomohiro , Noishiki Chikage , Fukuhara Shigetomo 
Published: March 2023

Abstract
Angiogenesis is a dynamic morphogenetic process that refers to the growth of new blood vessels from the pre-existing vessels and is critical for tissue repair during wound healing. In adult normal tissues, quiescent endothelial cells and pericytes maintain vascular integrity, whereas angiogenesis is immediately induced upon tissue injury, thereby forming neovascular networks to maintain homeostasis. However, impaired angiogenesis results in development of chronic and non-healing wounds in various diseases such as diabetes and peripheral artery diseases. Zebrafish are a vertebrate model organism widely used for studying many medical and life science fields. Indeed, the molecular and cellular mechanisms underlying regulation of wound angiogenesis have recently been studied by performing fluorescence-based live-imaging of adult zebrafish. In this review, we describe how endothelial cells and pericytes establish neovascular networks during wound angiogenesis and also introduce a novel role of blood flow-driven intraluminal pressure in regulating angiogenesis during wound healing.
Effect of nitrogen management in cultivation on the stability and microbial community of post-harvest Monoraphidium sp. algae biomass
Authors: Wahlen Bradley D , Wendt Lynn M , St. Germain Chelsea C , Traynor Sarah M , Barboza Caitlin , Dempster Thomas , Gerken Henri , McGowen John , You Yaqi 
Published: March 2023

Abstract
Long-term storage is necessary to mitigate for seasonal variation in algae productivity, to preserve biomass quality and to guarantee a constant biomass supply to a conversion facility. While ensiling has shown promise as a solution, biomass attributes for successful storage are poorly understood. Storage studies of Monoraphidium sp. biomass indicate a strong correlation between nitrogen management in algae cultivation and stability of post-harvest algae biomass. Algae cultivated with periodic nitrogen addition were stored poorly (&gt;20% loss, dry basis) compared to biomass from nitrogen depleted cultivation (8% loss, dry basis). A follow-up study compared the post-harvest stability of Monoraphidium biomass cultivated in nitrogen-deplete or nitrogen-replete conditions. Replete biomass experienced the largest degradation (24%, dry basis), while deplete biomass experienced the least (10%, dry basis). Dry matter loss experienced among blends of each correlated positively with nitrogen-replete biomass content. The composition of the post-storage algae microbial community was also affected by cultivation conditions, with Clostridia species being more prevalent in stored biomass obtained from nitrogen-replete cultivations. Nitrogen management has long been known to influence algae biomass productivity and biochemical composition; here, we demonstrate that it also strongly influences the stability of post-harvest algae biomass in anaerobic storage.
Analyzing CRISPR screens in non-conventional microbes
Authors: Trivedi Varun , Ramesh Adithya , Wheeldon Ian 
Published: March 2023

Abstract

The multifaceted nature of CRISPR screens has propelled advancements in the field of functional genomics. Pooled CRISPR screens involve creating programmed genetic perturbations across multiple genomic sites in a pool of host cells subjected to a challenge, empowering researchers to identify genetic causes of desirable phenotypes. These genome-wide screens have been widely used in mammalian cells to discover biological mechanisms of diseases and drive the development of targeted drugs and therapeutics. Their use in non-model organisms, especially in microbes to improve bioprocessing-relevant phenotypes, has been limited. Further compounding this issue is the lack of bioinformatic algorithms for analyzing microbial screening data with high accuracy. Here, we describe the general approach and underlying principles for conducting pooled CRISPR knockout screens in non-conventional yeasts and performing downstream analysis of the screening data, while also reviewing state-of-the-art algorithms for identification of CRISPR screening outcomes. Application of pooled CRISPR screens to non-model yeasts holds considerable potential to uncover novel metabolic engineering targets and improve industrial bioproduction.

One-Sentence Summary
This mini-review describes experimental and computational approaches for functional genomic screening using CRISPR technologies in non-conventional microbes.
Overcoming the challenges to implementation of artificial intelligence in pathology
Authors: Reis-Filho Jorge S , Kather Jakob Nikolas 
Published: March 2023

Abstract
Pathologists worldwide are facing remarkable challenges with increasing workloads and lack of time to provide consistently high-quality patient care. The application of artificial intelligence (AI) to digital whole-slide images has the potential of democratizing the access to expert pathology and affordable biomarkers by supporting pathologists in the provision of timely and accurate diagnosis as well as supporting oncologists by directly extracting prognostic and predictive biomarkers from tissue slides. The long-awaited adoption of AI in pathology, however, has not materialized, and the transformation of pathology is happening at a much slower pace than that observed in other fields (eg, radiology). Here, we provide a critical summary of the developments in digital and computational pathology in the last 10 years, outline key hurdles and ways to overcome them, and provide a perspective for AI-supported precision oncology in the future.
Intussusception of the appendix in a young adult: an important differential diagnosis of abdominal pain in cystic fibrosis patients?
Authors: Venkatasami Meena , Cobby Ellen 
Published: March 2023

AbstractCystic fibrosis (CF) is commonly associated with gastrointestinal manifestations from infancy to adulthood. Distal intestinal obstruction syndrome (DIOS) affects 20% of CF patients, where intussusception can be a rare complication. A 20-year-old CF male was diagnosed with a 3-day history of right iliac fossa pain and diarrhoea. Clinical examination revealed a tender palpable mass in the right iliac fossa and raised serum inflammatory markers. Contrast computerized-tomography of the abdomen-pelvis suggested intussusception of the appendix and further confirmed on histological analyses. The patient underwent an open appendicectomy where the intussusception had self-resolved. The literature review indicated a scarcity of data with 10 cases reported of intussusception in adult CF patients. Our case was in line with previous research of transient intussusception. This rare case highlights an importance to carry a higher index of suspicion for gastrointestinal manifestations in CF patients where differential diagnoses of DIOS and intussusception should be considered in the acute presentation.
Break-induced replication is the primary recombination pathway in plant somatic hybrid mitochondria: a model for mitochondrial horizontal gene transfer
Authors: Gandini Carolina L , Garcia Laura E , Abbona Cinthia C , Ceriotti Luis F , Kushnir Sergei , Geelen Danny , Sanchez-Puerta M Virginia 
Published: March 2023

Abstract
Somatic hybrids between distant species offer a remarkable model to study genomic recombination events after mitochondrial fusion. Recently, we described highly chimeric mitogenomes in two somatic hybrids between the Solanaceae Nicotiana tabacum and Hyoscyamus niger resulting from interparental homologous recombination. To better examine the recombination map in somatic hybrid mitochondria, we developed a more sensitive bioinformatic strategy to detect recombination activity based on high-throughput sequencing without assembling the hybrid mitogenome. We generated a new intergeneric somatic hybrid between N. tabacum and Physochlaina orientalis, and re-analyzed the somatic hybrids that we previously generated. We inferred 213 homologous recombination events across repeats of 2.1 kb on average. Most of them (~80%) were asymmetrical, consistent with the break-induced replication pathway. Only rare (2.74%) non-homologous events were detected. Interestingly, independent events frequently occurred in the same regions within and across somatic hybrids, suggesting the existence of recombination hotspots in plant mitogenomes. Break-induced replication is the main pathway of interparental recombination in somatic hybrid mitochondria. Findings of this study are relevant to mitogenome editing assays and to mechanistic aspects of DNA integration following mitochondrial DNA horizontal transfer events.
X-ray emission from Ae/Be Herbig stars due to disc–stellar magnetosphere interaction
Authors: Ryspaeva Elizaveta , Kholtygin Alexander , Lyutikov Maxim 
Published: March 2023

ABSTRACT
We reanalyse archival X-ray data of 16 Ae/Be Herbig stars obtained by the XMM–Newton and Chandra satellites. Stellar X-ray spectra in the energy range 0.2–8 keV were fitted with the use of APEC and MEKAL hot plasma emission models, and with models with an additional power-law component. We find that for Herbig stars, the dependence of the unabsorbed X-ray luminosity on stellar mass and radius, LX ∝ RαMβ with α ≈ 3 and β ≈ 2, is similar to that for T Tauri stars. The independently determined accretion rates, rotation periods, and the surface magnetic fields follow a tight correlation predicted by the standard magnetospheric accretion theory. We suggest that X-ray emission from Herbig stars is powered by magnetic reconnection events in the tenuous corona at the disc–magnetosphere boundary.