bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current Research on Chemical Composition, Analytical Methods, and Quality Standards of Amorphophallus konjac
Authors: Ting-ting Hu , Li Jin-yang 
Published: August 2023

Abstract
Amorphophallus konjac, a traditional Chinese medicine, possesses remarkable anti-tumor properties, exerting effects such as scattered stasis detumescence, detoxification, and pain relief. This medicinal herb is frequently employed in contemporary clinical settings to treat malignant tumors. However, the cultivation and processing of this herb lack standardisation, making it challenging to control its quality effectively. The current quality control standards for Amorphophallus sinensis Belval are not uniform, the detection methods are relatively limited, and the specificity is comparatively weak. Consequently, these standards fail to meet authenticity requirements, identify counterfeit products, and evaluate the quality of Amorphophallus sinensis Belval herbal materials and related medications. To enhance clinical efficacy, it is essential to review the existing analysis and identification methods as well as the quality control standards. This review aims to serve as a valuable resource for enhancing and optimising the quality evaluation system for Amorphophallus sinensis Belval, facilitating improved clinical outcomes.
Identification of a novel monopartite begomovirus associated with leaf curl disease of Citharexylum spinosum in India
Authors: Diksha Damini , Sidharthan V Kavi , Baranwal V K 
Published: August 2023

Abstract
The present study reports the complete genome of a novel monopartite begomovirus, named tentatively as “Citharexylum leaf curl virus” (CitLCuV), associated with leaf curl disease of Citharexylum spinosum in India. CitLCuV genome (2,767 nucleotide) contained the typical genome organization of Old World begomoviruses and shared the maximum nucleotide sequence identity of 89.2% with a rose leaf curl virus (RoLCuV) isolate. In addition, two small non-canonical open reading frames (C5 and C6) were determined in the complementary strand of CitLCuV genome. Phylogenetic analysis revealed the relatedness of CitLCuV to papaya leaf crumple virus and RoLCuV. Recombination analysis detected a possible recombination event in CitLCuV genome. Based on begomovirus species demarcation criteria, CitLCuV can be regarded as a novel begomoviral species.
Oculomotor Inhibition Markers of Working Memory Load
Authors: Kadosh Oren , Inbal Kfir , Snir Hadar , Bonneh Yoram 
Published: August 2023

Abstract
Involuntary eye movements occur constantly even during fixation and were shown to convey information about cognitive processes. They are inhibited momentarily in response to external stimuli (oculomotor inhibition, OMI), with a time and magnitude that depend on stimulus saliency, attention, and expectations. It was recently shown that the working memory load for numbers modulates the microsaccade rate; however, the generality of the effect and its temporal properties remain unclear. Our goal was to investigate the relationship between OMI and the working memory load for simple colored shapes. Participants (N = 26) maintained their fixation while their eyes were tracked; they viewed briefly flashed colored shapes accompanied by small arrows indicating the shapes to be memorized (1/2/3). After a retention period, a probe shape appeared for matching. The microsaccade rate modulation and temporal properties were analyzed for the memory encoding, maintenance, and retrieval phases. Microsaccade inhibition was stronger when more shapes were memorized, and performance improved when microsaccades were suppressed during maintenance and retrieval. This occurred even though the physical stimuli were identical in number under all conditions. Thus, oculomotor inhibition may play a role in silencing the visual input while processing current stimuli and is generally related to processing time and load.
Detecting Outliers in Ionospheric Correction Model for GNSS Precise Positioning
Authors: Dao Tam , Harima Ken , Carter Brett , Currie Julie , McClusky Simon , Brown Rupert , Rubinov Eldar , Barassi John , Choy Suelynn 
Published: August 2023

Abstract
Global Navigation Satellite System fast precise positioning can be achieved with accurate ionospheric corrections computed from an adequate number of GNSS stations in a local region. Our previous study showed that using 15 well-distributed GNSS stations within a 5° latitude x 10° longitude region, a local regression model for ionospheric corrections can achieve a mean accuracy of 5 cm. In low-latitude regions, the presence of electron density gradients over short distances can lead to outliers in the map of ionospheric corrections and decrease its accuracy. In this study, we explored outlier detection in ionospheric correction mapping through statistical residuals during a four-month test in 2021. Our findings indicate that the residuals of the local ionospheric model conform to the Laplace distribution. To determine outliers, we use an empirical rule for the Laplace distribution, setting thresholds at µ ± 3b, µ ± 3.5b, and µ ± 5.8b for data retention rates of 95%, 97%, and 99.7%, respectively. Here, µ represents the location parameter, which corresponds to the median of data, and b is the scale parameter, calculated as the medium absolute deviation. We found that while removing outliers can improve model accuracy, it may result in unavailable prediction due to a lack of data. For example, applying a µ ± 3.5b threshold for outlier removal led to approximately 2.5% of recording time having no ionospheric corrections map in low-latitude regions, however, the local model has the potential to improve its mean accuracy by up to 50% for both low and mid-latitudes.
Impact of graft kidney weight to recipient body weight ratio on long-term graft survival after living-donor kidney transplantation
Authors: Shin SeongWook , Lee Juhan , Yang Seok Jeong , Kim Hyun Jeong , Yang Jaeseok , Kim Beom Seok , Kim Myoung Soo , Huh Kyu Ha 
Published: August 2023

Abstract
Background
The relative size of kidney allografts is well known to be associated with graft function, but controversies remain about its effect on long-term graft survival. This study aimed to evaluate the effect of mismatch between graft kidney weight and recipient body weight on long-term graft survival.
Methods
We retrospectively evaluated 1397 consecutive adults undergoing live donor kidney transplantation between January 2000 and May 2019 at Severance Hospital, Republic of Korea. The patients were divided into 4 groups according to graft kidney weight to recipient body weight ratio (KW/BW) quartile. The median follow-up duration was 145 months.
Results
Kidney transplant recipients with a high KW/BW had significantly higher death-censored graft survival rates up to 10 years after transplantation than patients with a low KW/BW. On Cox multivariable regression analysis, pre-transplantation diabetes, older donor age, and 1st and 2nd KW/BW quartiles were independent risk factors for death-censored graft failure. The 1st KW/BW quartile had the lowest mean estimated glomerular filtration rate throughout the follow-up period.
Conclusions
Higher KW/BW was associated with better long-term graft function and graft survival after living-donor kidney transplantation.
Point Cloud Based Model-free Path Planning Method for grinding Large Complex Forging Parts
Authors: Yan Shouxin , Wang Wei , Su Pengfei , Wang Qilong , Zheng Lianyu 
Published: August 2023

Abstract
Large and complex forgings, serving as crucial load-supporting components in the fields of energy, ships, transportation, etc., demand high dimensional accuracy and surface quality during post-processing. The automation of grinding for such large and complex forgings presents a common and pressing challenge within the forging industry. One of the main obstacles lies in the substantial thermal deformation and various random forging defects, making the automatic generation of grinding paths a difficult task. In this paper, we propose an algorithm for identifying random defects in large and complex forgings. By combining the RANdom Sample Consensus (RANSAC) algorithm with the Modified Iterative Closest Point (M-ICP) algorithm, we register the standard component point cloud with the forging point cloud, thereby obtaining the point cloud representing the random defects that require grinding. Subsequently, we classify the random defect point cloud based on defect area size and establish a strategy for generating grinding paths. Utilizing the positional coordinate information within the random defect point cloud, we directly generate robot grinding paths without relying on a CAD model. Finally, we conduct robot grinding experiments on large and complex forgings. The experimental results demonstrate that the model-free generation method for grinding paths accurately identifies the characteristics of random forging defects, efficiently plans robot grinding paths, and significantly improves grinding efficiency and quality. This approach offers an intelligent solution for the post-processing of large and complex forgings.
Usefulness of impulse oscillometry in predicting the severity of bronchiectasis
Authors: Choi Ji Soo , Kwak Se Hyun , Kim Min Chul , Seol Chang Hwan , Heo Seok-Jae , Kim Sung Ryeol , Lee Eun Hye 
Published: August 2023

Abstract

Background: Bronchiectasis is a chronic respiratory disease that leads to airway inflammation, destruction, and airflow limitation, which reflects its severity. Impulse oscillometry (IOS) is a non-invasive method that uses sound waves to estimate lung function and airway resistance. The aim of this study was to assess the usefulness of IOS in predicting the severity of bronchiectasis.
Methods: We retrospectively reviewed the IOS parameters, clinical characteristics, and results of other examinations in 145 patients diagnosed with bronchiectasis between March 2020 and May 2021. Disease severity was evaluated using the FACED score, and patients were divided into mild and moderate/severe groups.
Results: Forty-four patients (30.3%) were in the moderate/severe group, and 101 (69.7%) were in the mild group. The mean predicted forced expiratory volume in one second was 78.0% in the mild group and 54.5% in the moderate/severe group. Patients with moderate/severe bronchiectasis had a higher airway resistance at 5 Hz (R5) (0.53 vs. 0.41, p <0.001), a higher difference between the resistance at 5 and 20 Hz (R5 − R20; 39.85 vs. 26.81, p <0.001), a higher resonant frequency (Fres) (22.52 vs. 17.54, p <0.001), and a higher reactance area (AX) (2.44 vs. 1.06, p <0.001) than patients with mild bronchiectasis. R5 ≥0.43, R20 ≥0.234, R5 − R20 ≥28.3, AX ≥1.02, reactance at 5 Hz (X5) ≤−0.238, and Fres ≥20.88 revealed significant univariable relationships with bronchiectasis severity (p-values <0.05). Among these, only X5 ≤−0.238 exhibited a significant multivariable relationship with bronchiectasis severity (p = 0.039). The receiver operating characteristic curve for the severity of bronchiectasis based on IOS parameters exhibited an area under the curve of 0.809.
Conclusions: The IOS parameters can effectively reflect airway resistance and elasticity in patients with bronchiectasis and serve as valuable tools for predicting bronchiectasis severity.
Assessing genetic diversity of soybean based on smartphone image-derived canopy parameter
Authors: Ri Myong-Kwang , Jong Kwang-O , Sin Ye-Kwang , Jang Yu-Mi , Ri Kum-Sil , Ho Dal , Kim Song-Kuk 
Published: August 2023

Abstract
Convenient and accurate characterization of field-grown crops is an urgent need not only for effective use of germplasm resources but also further for breeding programs. In this study, smartphone image-derived canopy parameter was utilized to study the genetic relationships among 18 soybean accessions at early growing period. Field experiments were conducted in two consecutive years (2021 and 2022). Canopy cover rate (CCR) per plant that is canopy parameter and relative indicator were estimated using image analysis software from top-view image without any significant alteration of plant morphology. CCR can be used as the indirect and comprehensive index for investigating morphological and biomass traits at early growing period in field-grown soybean accessions. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and discriminant analysis (DA) were performed for grouping soybean accessions. For DA, average of percentage of correctly classified on the basis of CCR was 88.9% of grouped cases by PCA. Results of HCA based on CCR were more similar to the ones suggested by the PCA than ones of clustering based on morphological and biomass traits. Overall results suggest that CCR can be used as the useful index for diversity in field-grown crop genotypes. This study provides not only an innovative method for comprehensive evaluation of crop genetic resources using image-derived parameter, but also an approach to promote genetic improvement of crop varieties.
Biological studies of a substituted bipyridine-coordinated Zn(II) ion: In vitro cytotoxicity, ROS-induced apoptosis, anti-metastatic and nuclease-like activity plus in silico binding affinity towards cancer-related proteins
Authors: Anjomshoa Marzieh , Sahihi Mehdi , Janczak Jan , Karami-Mohajeri Somayyeh , Farsinejad Alireza , Arabnezhad Mohammad-Reza , Amirheidari Bagher 
Published: August 2023

Abstract

A new dimethyl-substituted bipyridine-Zn(II) complex was synthesized and structurally characterized. Single-crystalline structure of the complex was successfully elucidated as [Zn(2Mebpy)3](ClO4)2•1.5(dioxane) by X-ray diffraction, where 2Mebpy represents 4,4′-dimethyl-2,2′-bipyridine. Also, the three-dimensional electrostatic potential maps (3D ESP) were plotted for [Zn(2Mebpy)3]2+ cation and [Zn(2Mebpy)3](ClO4)2 molecule. The in vitro cytotoxicity of the complex, compared with cisplatin, was evaluated towards the human breast (MCF-7) and glioblastoma (U-87MG) cancer cells as well as normal murine embryo cells (NIH/3T3). Noteworthily, great cytotoxicity and selectivity of the Zn(II) complex against MCF˗7 cells was confirmed by IC50 value of 5.1±0.5 μM and selectivity index of 3.2 over 48 h. The complex-induced MCF-7 and U-87 cells death mostly proceeded through an apoptotic pathway in a dose-dependent manner as evidenced by the flow cytometric results. The complex demonstrated potential anti-metastatic activity against MCF-7 cells as evidenced by inhibition of colony formation and cell migration. The apoptotic mechanism in the complex-treated MCF-7 cells was probably associated with the overproduction of reactive oxygen species (ROS). The comet assay results also showed that the complex could induce DNA damage in MCF-7 cells. Moreover, the complex exhibited hydrolytic cleavage of pUC19 DNA, which may be responsible for their observed cytotoxic effect to some extent. Finally, binding affinity of the complex towards cancer therapeutic targets such as anti-apoptotic proteins, estrogen receptor α, tubulin, and topoisomerase II was predicted by in silico molecular docking. In conclusion, the complex can be introduced as a potential therapeutic agent in breast cancer. This work indicate that other metal-cored complexes baring bypyridine derivatives can also exhibit promissing anticancer effects, opening the window towards a battery of similar candidate structures.
Muscle transcriptome sequencing revealed thermal stress responsive regulatory genes in farmed rohu, Labeo rohita (Hamilton, 1822)
Authors: Pokanti Vinay Kumar , Rasal Kiran D. , Acharya Arpit , Dey Diganta , Sonwane Arvind A. , Reang Dhalongsaih , R Rajeshkannan , Pawar Sachin S. , Kurade Nitin P. , Bhendarkar Mukesh P. , Krishnani Kishore K. , Nagpure Naresh S. , Brahmane Manoj P. 
Published: August 2023

Abstract
Rohu, Labeo rohita is one of the most important aquaculture species in the Indian subcontinent. The physiological responses to thermal stress or climate change at the molecular level are less understood in rohu. In the present work, transcriptome sequencing was carried out in muscle tissue of the rohu in response to heat stress (35ºC) in comparison with the control (28ºC). A total of 125 Gb of sequence data was generated and the raw-reads were filtered and trimmed, that resulted in 484 million quality reads. Reference-based assembly of reads was performed using L. rohita genome and a total of 90.17% of reads were successfully mapped. A total of 37,462 contigs were assembled with an N50 value of 1854. The differential expression analysis revealed a total of 107 differentially expressed genes (DEGs) (15 up-, 37 down-, and 55 neutrally-regulated) as compared to the control group (Log2FC>2, P<0.05). Gene enrichment analysis of DEGs indicates that transcripts were associated with molecular, biological, and cellular activities. The randomly selected differentially expressed transcripts were validated by RT-qPCR and found consistent expression patterns in line with the RNA-seq data. Several transcripts such as Serpine 1(HSP47), HSP70, HSP90alpha, Rano class II histocompatibility A beta, PGC-1 and ERR-induced regulator, Proto-oncogen c-fos, myozenin2, Alpha-crystallin B chain-like protein, Angiopoietin-like protein 8, Acetyl-CoA carboxylases, etc. have been identified in muscle tissue of rohu that are associated with stress/immunity. This study identified the key biomarker Serpine 1(HSP47), which showed significant up-regulation (~2 to 3-fold) in muscle tissue of rohu exposed to high temperature. This study can pave a path for the identification of stress-responsive biomarkers linked with thermal adaptations in the farmed carps.
Proposal of a diagnostic algorithm for radiation-induced dropped head syndrome in long-term childhood cancer survivors based on a prospective study in a specialized clinical setting and a review of the literature
Authors: Rieken Sarah , Kronziel Lea Louisa , Langer Thorsten , Boppel Tobias , Trillenberg Peter , Gebauer Judith 
Published: August 2023

Abstract
Purpose
To prospectively assess incidence of Dropped Head Syndrome (DHS) in childhood cancer survivors (CCS) and to develop and evaluate a diagnostic algorithm for DHS
Methods
A systematic literature search for DHS in combination with neck radiotherapy (RT) exposure was performed. Analyses and combination of the most common examination methods were integrated into a diagnostic algorithm. All CCS visiting the local late effects clinic between May 2020 and April 2022 were included in the study. CCS exposed to neck RT with ≥ 19 Gray (Gy) received standardized clinical and neurological assessment and, in CCS with abnormal results, an MRI scan to confirm muscle atrophy.
Results
206 CCS were included of which 42 were exposed to neck RT ≥ 19 Gray (Gy). In the overall cohort and in the subgroup following RT, 2.4% and 12% of CCS were affected by DHS, respectively. Results of clinical and neurological assessment correlate well with MRI results. Neck circumference and neck/thigh ratio were lower after neck RT. Over 50% of CCS experienced neck disability and pain.
Conclusions
A relevant proportion of CCS exposed to neck RT is affected by DHS. High concordance of MRI results with the neurological examination supports the clinical value of the diagnostic algorithm. Measurement of neck circumference might be an easy tool for assessment of neck muscle atrophy in survivors at risk.
Implications for Cancer Survivors
Integration of a diagnostic algorithm for DHS in standard long-term follow-up care facilitates diagnosis as well as initiation of early treatment and obviates the need for invasive examinations.
Eculizumab as rescue therapy in a context of dramatic NMOSD attack: report of two cases
Authors: San-Galli Aurore , Chaumont Hugo , Bourgeois Quentin , Roger Juliette , Lobjois Quentin , Cabre Philippe 
Published: August 2023

Abstract
Early administration of plasma exchanges (PE) combined with intravenous methylprednisolone (IVMP) is considered the best treatment for neuromyelitis optica spectrum disorder (NMOSD) attack. However, up to 20% of patients fail to respond, suggesting the existence of idiosyncratic factors yet to be understood. We report cases of two women who suffered devastating Aquaporin-4 immunoglobulin G-positive (AQP4-IgG+) NMOSD attacks, worsening despite optimal treatment up to life-threatening, for which eculizumab was successfully administrated as a rescue therapy. The first case describes a fulminant onset of the disease with pan-medullary and bulbar lesions leading to tetraplegia and respiratory failure within a few days, directly refractory to PE/IVMP. The second case described the 4th attack within two years of an aggressive disease, currently treated with mycophenolate mofetil, with early post-mitoxantrone relapse. For both patients, acute administration of eculizumab immediately after usual treatment failure seemed to have rapidly aborted the inflammatory cascade, saving them from imminent death. In addition to its proven efficacy in preventing relapses in AQP4-IgG + NMOSD, eculizumab could also rapidly stop an attack before the installation of irreversible lesions or death. This raises therapeutic issues relative to the management of such complement inhibitor treatment as rescue therapy, and questions about pathophysiological mechanisms of resistance to PE.
A unique role for Clostridium perfringens in the digestion of HMOs
Authors: McDonald Andrew , Lisacek Frederique 
Published: August 2023

Abstract
Background: The development of a stable human gut microbiota occurs within the first year of life. Many open questions remain about how microfloral species are influenced by the composition of milk, in particular its content of human milk oligosaccharides (HMOs). Objectives: The objective is to investigate the effect of the human HMO glycome on bacterial symbiosis and competition, based on the glycoside hydrolase (GH) enzyme activities known to be present in microbial species.
Methods: We extracted from UniProt a list of all bacterial species catalysing glycoside hydrolase activities (EC 3.2.1.-), cross-referencing with the BRENDA database, and obtained a set of taxonomic lineages and CAZy family data. A set of 13 documented enzyme activities was selected and modelled within an enzyme simulator according to a method described previously in the context of biosynthesis.
Results: A diverse population of experimentally observed HMOs was fed to the simulator, and the enzymes matching specific bacterial species were recorded, based on their appearance of individual enzymes in the UniProt dataset. Pairs of bacterial species were identified that possessed complementary enzyme profiles enabling the digestion of the HMO glycome, from which potential symbioses could be inferred. Conversely, bacterial species having similar GH enzyme profiles were considered likely to be in competition for the same set of dietary HMOs within the gut of the newborn. We generated a set of putative biodegradative networks from the simulator output, which provides a visualisation of the ability of organisms to digest HMO and mucin-type O-glycans. B. bifidum, B. longum and C. perfringens species were predicted to have the most diverse GH activity and therefore to excel in their ability to digest these substrates. The expected cooperative role of Bifidobacteriales contrasts with the surprising capacities of the pathogen.
Conclusions: These findings indicate that potential pathogens may associate in human gut based on their shared glycoside hydrolase digestive apparatus, and which, in the event of colonisation, might result in dysbiosis. The methods described can readily be adapted to other enzyme categories and species as well as being easily fine-tuneable if new degrading enzymes are identified and require inclusion in the model.