bluuebunny/crossref_metadata_2025_split huggingface dataset:
POLR2J is a potential biomarker for abnormal tumor progression, vorinostat sensitization, immune infiltration, and prognosis of glioblastoma multiform
Authors: Li Zhi-di , Liu Ye-han , Shen Shu-ying , Shen Fei-yan , Li Wan-yan , zhang chong , Zeng Ling-hui 
Published: January 2023

Abstract
Glioma is one of the most primary malignant brain tumors, and glioblastoma multiform (GBM) is the most common and highly aggressive glioma. Most GBM are high malignant, poor prognosis, resistant to conventional therapy, and prone to recurrence. Therefore, it is crucial to explore novel therapeutics strategies for the treatment and prognosis of GBM. In this study, we elucidated that the maximal overexpression of DNA-directed RNA polymerase II subunit J-1 (POLR2J) was observed in GBM compared with normal tissues among all cancer types, and high expression of POLR2J or its co-expressed genes predicted poor outcome of GBM patients. DNA replication were significantly enriched in the GBM clinical samples with high POLR2J expression, and POLR2J suppression inhibited the proliferation and triggered cell cycle G1/G0 phase arrest of GBM cells. HDAC inhibitors, such as vorinostat, are identified as effective agents against GBM. We showed that POLR2J silence activated UPR and significantly enhanced anti-GBM activity of vorinostat via suppressing cell proliferation and inducing apoptosis. In addition, POLR2J promoted epithelial-mesenchymal transition (EMT) and the metastatic potentials of GBM cells. Furthermore, POLR2J expression was negatively relevant to the number of B cells, neutrophil, myeloid dendritic cells, CD4 + T cells and etc. Meanwhile, the expression of POLR2J was negatively correlative to the expression of immunotherapy-related genes. Our study confirmed a novel oncogene POLR2J in GBM progression as well as provided a promising strategy for the chemotherapy and immunotherapy of GBM treatment.
The role of CXCL1 in crosstalk between endocrine resistant breast cancer and fibroblast
Authors: Pandithar Sneha , Galke Daniel , Akume Ahone , Belyakov Artem , Lomonaco Dominick , Guerra Amirah A. , Park Jay , Reff Olivia , Jin Kideok 
Published: January 2023

Abstract
Background: ER positive breast cancer is currently targeted using various endocrine therapies. Despite the proven therapeutic efficacy, resistance to the drug and reoccurrence of tumor appears to be a complication that many patients deal with. Molecular pathways underlying the development of resistance are being widely studied.
Methods and results: In this study, using four established endocrine resistant breast cancer (ERBC) cell lines, we characterized CXCL1 as a secreted factor in crosstalk between ERBC cells and fibroblasts. Protein array revealed upreguation of CXCL1 and we confirmed the CXCL1 expression by real-time qRT-PCR and U-Plex assay. Co-culturing ERBC cells with fibroblasts enhanced the cell growth and migration compared to the monoculture. The crosstalk of ERBC cells with fibroblasts significantly activates ERK/MAPK signaling pathway while reparixin, CXCR1/2 receptor inhibitor, attenuates the activity. Reparixin displayed the ERBC cell growth inhibition and the combination treatment with reparixin and CDK4/6 inhibitor (palbociclib and ribociclib).
Conclusions: Taken together, our study implicates CXCL1 as a critical role in ERBC growth and metastasis via crosstalk with fibroblast and cotargeting CXCR1/2 and CDK4/6 could potentially overcome endocrine resistant breast cancer.
Analysis of agronomic traits and regulatory mechanism of a semi-dwarf, albino and blue grain wheat line
Authors: Sun Fengli , Ye Wenjie , Li Song , Wang Zhulin , Xie Kunliang , Wang Weiwei , Zhang Chao , Xi Yajun 
Published: January 2023

Abstract
Plant height and leaf color are important agronomic traits in crops, since they contribute to the production of grains and biomass. Progress has been made mapping the genes that regulate plant height and leaf color in wheat and other crops. We created the wheat (Triticum aestivum L.) line DW-B (Dwarfing, White leaves and Blue grains) with semi-dwarfing and albinism at the tillering stage and re-greening at the jointing stage using Lango and Indian Blue Grain. Transcriptomic analyses of the three wheat lines at the early jointing stages indicated that the genes of gibberellin (GA) signaling pathway and chlorophyll biosynthesis were expressed differently in DW-B and its parents. The results of measurement of the response of GA and chlorophyll contents showed differences between DW-B and its parents. This study provides a comprehensive understanding of the regulation of plant height and leaf color.
Bioconvection of unsteady nonhomogeneous hybrid nanofluids influenced by motile microorganisms
Authors: Mabrouk S. M. , Inc Mustafa , Rashed A. S. , Akgül Ali 
Published: January 2023

Abstract
Motile bacteria in hybrid nanofluids cause bioconvection. Bacillus cereus, Pseudomonas viscose, Bacillus brevis, Salmonella typhimurium, and Pseudomonas fluorescens were used to evaluate their effect and dispersion in the hybrid nanofluid. Using hybrid Al2O3 and Cu nanoparticles (Cu-Al2O3/water), a two-phase model for mixed bioconvection magnetohydrodynamic flow was created using similarity analysis. M, Pr, and k_p* all affect the sensitivity of velocity and shear stresses. Magnetic, radiation and chemotaxis factors impact bacterial density. Increasing temperature differential and bacterium diffusivity increase bacterial aggregation.
Appraisal of trace metals toxicity and human health risk using a novel approach in produced water of four gas fields, Bangladesh.
Authors: Hossain Md. Numan , Howladar M. Farhad 
Published: January 2023

Abstract
The current research was conducted in the Sylhet gas-fields area of Bangladesh to evaluate the toxicity of trace metals (TMs) and associated health risks. Gas well-produced water, gas field discharge water, and drinking water surrounding the gas fields were collected for analysis. This study analyzed both carcinogenic and noncarcinogenic TMs such as Cr, As, Co, Cd, Fe, Ni, Mn, Zn, and Pb, as well as physical parameters pH and EC in the collected samples. The Fe and EC concentrations in produced and discharged water samples were found to be incredibly high. The average concentrations of TMs in produced water samples during the summer and rainy season were found to be: Fe > Mn > Zn > Ni > Co > Cu > Cr > Pb > As > Cd. The observed metal concentration in discharge water was higher during the summer than rainy season. Most samples met national and international standards for trace elements, while a few samples exceeded the threshold value. The Heavy Metal Evaluation Index (HMEI) results indicate that the majority of produced water is highly polluted, while discharge and drinking water are moderately to lowly polluted. The Environmental Pollution Index (EPI) indicates environmental pollution for some produced water samples, while it indicates a moderate to low risk for discharge and drinking water samples. The source allocation of heavy metals (HMs) was determined using positive matrix factorization. However, all water samples have an ecological risk index of less than 150, indicating lower risk. Furthermore, the Heavy Metals Toxicity Load (HMTL) was found to be below the allowable toxicity load for all water samples, indicating minimal contamination in the studied water. The non-cancer hazard score (HI) of the studied HMs is greater for children and females than for males. The cancer risk from Ni is a concern, while the cancer risk from Cd and Cr is also a matter of concern.
Synergistic effects of marine pollutants and microplastics on the destabilization of lipid bilayers
Authors: fleury jean baptiste , Baulin Vladimir A. 
Published: January 2023

Abstract
The increasing production of plastic worldwide results in increasing amounts of plastic waste dumped into the oceans. Plastic waste is fragmenting into smaller pieces up to micro and nanoplastics, which may cross physiological barriers and affect the functions of living organisms. Microplastics can travel with rain and clouds; they are present in soil, snow-capped mountains, and even in the organs and blood of human beings. The potential health implications of microplastics in living organisms raise concerns and are intensively investigated. Recently, we reported the effect of mechanical stretching of microplastics on a lipid bilayer. By combining experimental and theoretical approaches, we have shown that microplastic particles adsorbed on lipid membranes increase membrane stress even at low particle concentrations. In this manuscript, we demonstrate the synergetic effect of marine pollutants on the mechanical interaction induced by microplastics. For this purpose, bare microplastics are incubated in seawater containing marine pollutants. We show that pollutants, such as chemical sol- vents, significantly increase the mechanical stretching induced by microplastics. In turn, microplastics can be viewed as vectors for solvent molecules, facilitating their penetration into the core of lipid membranes and thus strongly affecting their biophysical properties.
Molecular screening of Amblyomma species (Acari: Ixodidae) in Egypt, with first report of the snake tick Amblyomma latum Koch, 1844
Authors: Abouelhassan Eman M. , Kamel Marwa S. , Chitimia‑Dobler Lidia , Bakkes Deon K. , Okely Mohammed 
Published: January 2023

Abstract
Amblyomma species are non-endemic ticks in Egypt, which have been recorded from imported animals. This study was carried out in 2022 to monitor Amblyomma spp. from dromedary camels, cattle, and snakes in Egypt. During this study, 400 camels, 200 cattle, and two snakes (Pythonidae) were inspected for tick infestation. Collected specimens were identified based on morphological characters and confirmed by phylogenetic analysis of the 12S rRNA gene. Camels were infested by adult specimens of Amblyomma variegatum and Amblyomma lepidum, but no Amblyomma spp. were collected from cattle. Amblyomma variegatum showed high genetic similarity to other A. variegatum from Guinea-Bissau and São Tomé (99.996%), and A. lepidum showed high genetic similarity to other A. lepidum from Israel and Sudan (99.986%). Amblyomma latum is recorded in Egypt from African snakes for the first time and showed high genetic similarity with South African A. latum (99.867%).
Effects of intrauterine exposure to SARS-CoV-2 on infants’ development: A rapid review and meta-analysis
Authors: Pinheiro Gabriela Soutto Mayor Assumpção , Souza Rayany Cristina , Azevedo Vivian Mara Gonçalves Oliveira , Guimarães Nathalia Sernizon , Pires Laura Gregório , Lemos Stela Maris Aguiar , Alves Claudia Regina Lindgren 
Published: January 2023

Abstract
Background
Potential medium and long-term neurodevelopmental sequelae of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during pregnancy have not been ruled out. Thus, we aimed to systematically review and summarize the evidence concerning the effects of intrauterine exposure to SARS-CoV-2 on infants’ development and behavior.
Methods
Scopus, PubMed, Web of Science, CINAHL, and PsycNet databases were searched for studies published up to November 04, 2022, investigating the repercussions of gestational SARS-CoV-2 on infants’ development and behavior. We performed a narrative synthesis according to updated protocols. Studies using comparison groups and with the Ages and Stages Questionnaires-Third Edition (ASQ-3) scores available were included in a meta-analysis performed according to Cochrane protocols. We used the Newcastle-Ottawa Quality Assessment Scale to analyze the risk of bias. Heterogeneity was calculated using the I2 statistic.
Results
The search identified 2,552 studies. After removing duplicates and applying the eligibility criteria, we performed a narrative synthesis on eight included studies and a meta-analysis on three. There was no evidence of higher developmental delay rates in infants exposed to SARS-CoV-2 during pregnancy compared to non-exposed infants. However, the exposed infants scored lower than either of non-exposed children and pre-pandemic cohorts in some domains. Pooled results from the random-effects model indicated that SARS-CoV-2-exposed infants had lower scores on fine motor (mean difference [MD]=-4.70, 95% confidence interval [CI]: -8.76; -0.63), and problem-solving (MD=-3.05, 95% CI: -5.88; -0.22) domains than non-exposed (heterogeneity: I2 = 69% and 88%, respectively). There was no difference between exposed and non-exposed infants in the communication, gross motor, and personal-social ASQ-3 domains.
Conclusions
We did not find evidence confirming the association between SARS-CoV-2 gestational exposure and neurodevelopmental delay. However, the meta-analysis indicated that gestational exposure negatively affected fine motor and problem-solving skills. Robust evidence on the topic is still incipient, and the available studies present methodological inconsistencies that limit the drawing of clear-cut conclusions.
Novel organoid construction strategy for non-involuting congenital hemangioma for drug validation
Authors: Wei Haoche , Li Yanan , Li Li , Hu Qian , Shi Mingsong , Cheng Linbo , Jiang Xile , Zhou Yanting , Chen Siyuan , Ji Yi , Cheng Lijuan 
Published: January 2023

Abstract
Background:Non-involuting congenital hemangiomas (NICHs) are fully formed vascular tumors at birth, with distinctive clinical, radiologic, and histopathological profiles. In the literature, there is no effective therapy strategy for patients with NICH except surgery. Currently, no cell line or animal model exists for studying the mechanism of NICH and drug validation. We plan to construct a new strategy by constructing NICH organoids for further study.
Result:Here, we report a novel NICH organoid system construction and optimizationprocess. Both HE and immunohistological staining exactly matched NICH tissue. We further performed transcriptome analysis to elucidate the characteristics of NICH organoids. Both NICH tissue and NICH organoids manifested similar trends in download sites. NICH organoids display novel features to new cells derived from organoids and show spectacular multiplication capacity. In the preliminary verification, we found that cells splitting from NICH organoidswere human endothelial cells. Drug validation demonstrated that trametinib, sirolimus, and propranolol showed no inhibitory effects on NICH organoids.
Conclusion: Our data show that this new NICH-derived organoid faithfully captured the features of this rare vascular tumor. Our study will boost further research on the mechanism of NICH and drug filtering in the future.
The first chick brain with non-invasively embedded beads: a foundation for the automation of brain research
Authors: Yoshimura Akari , Seki Masayuki 
Published: January 2023

Abstract
Background
The automation of biotechnology, such as next-generation DNA sequencing, revolutionarily provides massive amounts of data and integrates various research fields. By contrast, many non-automated brain research fields are not interconnected with one other. In this study, we developed a basis for the automation of brain research. Two major technical barriers for the automation of brain research in vertebrates are the necessity for skull incision and a precise inoculation system for probes, devices, and electrodes in defined brain locations.
Methods
The former barrier in the background was overcome by inoculating probes into the future brain area of chick embryos before skull formation.
Results
Fluorescent micro-beads that mimic probes were inoculated into the future brain area of chick embryos, and 20% of the manipulated embryos hatched, with 71% of the hatched chicks containing multiple beads in their brains.
Conclusion
With this technique, beads are embedded inside the brain without skull incision, promising a novel non-invasive method that overcomes the drawbacks associated with traditional invasive brain manipulation.
Fuzzy Cost Optimization for Single Server Queue under Admission Control F-policy and Balking
Authors: Sanga Sudeep Singh , Antala Khushbu 
Published: January 2023

Abstract
This paper deals with the finite capacity unreliable single server queueing model under admission control F-policy with balking and feedback. F-policy deals with controlling the arriving customers in the system. In F-policy, if the system capacity is full (say K) then further customer is prohibited from admission to the system. The new admission of the customer is allowed into the system when system capacity decreases to a threshold value F (0 ≤ F ≤ K − 1). The concept of balking is incorporated to reduce the waiting line of the customer. The idea of server breakdown and repair is also added. The steady-state system probabilities and several performance indices are derived by using the Gauss-Seidel method. We construct the cost function and minimize it by using the direct search method. The cost minimization corresponding to the optimal service rate is also presented. We also present the numerical result under the optimal operating condition.
Implications of tioguanine dosing in IBD patients with a TPMT deficiency
Authors: Deben Debbie S. , Derijks Luc J.J. , Bosch Bianca J.C. van den , Creemers Rob H. , Nunen Annick van , Bodegraven Adriaan A. van , Wong Dennis R. 
Published: January 2023

Abstract
Purpose: Tioguanine is metabolised by less enzymatic steps compared to azathioprine and mercaptopurine, without generating 6-methylmercaptopurine ribonucleotides. However, thiopurine S-methyl transferase (TPMT) plays a role in early toxicity in all thiopurines. We aimed to describe the hazards and opportunities of tioguanine use in inflammatory bowel disease (IBD) patients with aberrant TPMT metabolism and propose preventative measures to safely prescribe tioguanine in these patients.
Methods: In this retrospective cohort study, all determined TPMT genotypes (2016 – 2021) were evaluated for aberrant metabolism (i.e. intermediate and poor TPMT metabolisers). Subsequently, all IBD patients on tioguanine with aberrant TPMTgenotypes were evaluated for tioguanine dosages, adverse drug events, lab abnormalities, treatment duration and effectiveness.
Results: TPMT genotypes were determined in 485 patients of whom 50 (10.3%) and 4 patients (0.8%) were intermediate and poor metabolisers, respectively. Of these patients, 12 intermediate and 4 poor TPMT metabolisers had been prescribed tioguanine in varying doses. In one poor TPMT metaboliser, tioguanine 10 mg/day induced delayed pancytopenia.
In general, reduced tioguanine dosages of 5 mg/day for intermediate TPMT metabolisers, and 10 mg two-weekly for poor TPMT metabolisers, resulted in a safe, long-term treatment strategy.
Conclusions: Diminished or absent TPMT enzyme activity was related with a pharmacokinetic shift of tioguanine metabolism which is associated with relatively late occurring myelotoxicity in patients on standard tioguanine dose. However, in strongly reduced dose regimens with strict therapeutic drug and safety monitoring, tioguanine treatment remained a safe and effective option in IBD patients with dysfunctional TPMT.
A nomogram combining NLR, PLR and SII to predict progression-free survival of cetuximab-based first-line therapy in patients with metastatic colorectal cancer
Authors: Su Rixin , Shen Hao , Dang Wenxi , Liu Xudong , Zhang Zhihua , Wu Shusheng , Li Mengge , He Yifu 
Published: January 2023

Abstract
Objective: To establish an effective prognostic nomogram combining neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) to predict progression-free survival (PFS) of metastatic colorectal cancer (mCRC) patientstreated with cetuximab-based first-line therapy.
Materials and methods: 143 patients with mCRC were admitted to our hospital and received cetuximab-based first-line therapy. The patients were separated into training and validation cohorts. Patients' baseline blood parameters and clinical characteristics were collected. In the training cohort, Kaplan-Meier analysis, univariate analysis, and multivariate analysis were used to identify factors associated with PFS and to screen for independent predictors. A prognostic nomogram was constructed, and the model's predictive efficacy, stability and net benefit were evaluated. Validation was performed in the validation cohort.
Results: Kaplan-Meier analysis showed that patients in the NLR≥3.9 group, PLR≥152.2 group and SII≥464.3 group had worse PFS than those in the NLR<3.9 group, PLR<152.2 group and SII<464.3 group, respectively (P<0.001). Univariate analysis revealed that the resected primary tumor, liver metastases, NLR, PLR and SII were significantly correlated with PFS. Multivariate analysis revealed that the resected primary tumor (HR: 0.551, 95%CI: 0.329-0.924, P=0.024), liver metastases (HR: 2.033, 95%CI: 1.212-3.407, P=0.007), NLR (HR: 2.596, 95%CI: 1.378-4.888, P=0.003), PLR (HR: 2.002, 95%CI: 1.235-3.246, P=0.005) and SII (HR: 2.202, 95%CI: 1.292-3.751, P=0.004) were independent prognostic factors affecting PFS. A prognostic nomogram model was developed and revealed the greatest predictive efficacy (AUC=0.870). The nomogram revealed excellent stability and predictive value in both training (C-index=0.827) and validation cohort (C-index=0.870). Decision curve analysis (DCA) proved that the prognostic nomogram could be clinically valuable. Conclusions: The nomogram combining the resected primary tumor, liver metastases, NLR, PLR, and SII can be used to predict the PFS of mCRC patients treated with cetuximab-based first-line therapy.
Microbiome resilience of three-toed box turtles (Terrapene carolina triunguis) in response to rising temperatures
Authors: Guan Jimmy , Ramírez Gustavo A. , Eng Curtis , Oakley Brian 
Published: January 2023

Abstract
Background
The gastrointestinal (GI) microbiome of chelonians plays an important role in their metabolism, nutrition, and overall health but the GI microbiome of three-toed box turtles (Terrapene carolina triunguis) has yet to be characterized. How the GI microbiome responds to rapidly rising environmental temperatures has also not been studied extensively in ectotherms, specifically chelonians. Ambient temperature increases are known to perturb host-associated microbial communities and can result in dysbiosis and possible shedding of pathogens. In this study, twenty (20) T.c.triunguis were split into control and experimental groups. The experimental group experienced 4.5°C increases every two weeks while the control group stayed at a constant ambient temperature (24°C) through the entirety of the experiment. Before each temperature increase, all turtles had cloacal swab samples taken. These samples underwent DNA extraction followed by 16S rRNA gene sequencing and microbial community analyses.
Results
Community composition of both groups were primarily composed of members of the classes Alphaproteobacteria, Gammaproteobacteria, Bacteroidia, Clostridia, and Bacilli. Differences in diversity at the community level in the controls compared to the experimental groups were not significant, indicating microbiome resilience to rapid temperature changes in T.c.triunguis, although some differentially abundant genera were identified. Interestingly, an amplicon sequence variant belonging to the Erysipelothrix spp. was exclusively enriched in the highest temperature group relative to controls.
Conclusions
Although there were significant differences in both ambient and body temperature in the experimental group, there was not a significant change in diversity of the microbiome. This suggests that there may be an innate robustness to rapid temperature swings in the microbiome of T.c.triunguis which are native to temperate North America. Despite this resilience, a sequence type of Erysipelothrix spp. was enriched at the highest temperature. Phylogenetic analysis of this amplicon variant showed that it is a close relative of Erysipelothrix rhusiopathiae, a pathogen of zoonotic importance associated with both wildlife and livestock.