bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic Wound Milieu Challenges Essential Oils’ Antibiofilm Activity
Authors: Brożyna Malwina , Kozłowska Weronika , Malec Katarzyna , Paleczny Justyna , Detyna Jerzy , Fabianowska-Majewska Krystyna , Junka Adam 
Published: June 2023

AbstractThe treatment of infected non-healing wounds poses a significant challenge to contemporary medicine. Essential oils (EOs) are being increasingly investigated as potential antibiofilm agents for the management of biofilm-related wound infections. However, theirin vitroantimicrobial activity reported in literature does not necessarily reflect the actualin vivoactivity due to, among others, methodological imperfections of performed tests. In our study, we cultivated aStaphylococcus aureusbiofilm in a novel IVWM (In Vitro Wound Milieu) medium designed to simulate the wound environment and compared it with biofilms cultured in the standard microbiological Tryptic Soy Broth (TSB) medium. We examined and compared critical biofilm properties such as morphology, biomass, metabolic activity, cell count, thickness, and spatial distribution of live and dead cells. Subsequently, staphylococcal biofilms and planktonic cells cultured in both media were exposed to the activity of thyme or rosemary EOs (T-EO, R-EO, respectively). We found that morphology of biofilms cultured in IVWM resembled more the morphology of biofilms visualized in the non-healing wounds than biofilms cultured in TSB. The biomass, metabolic activity, cell number, and the ratio of live to dead cells ofS. aureusbiofilms were all lower in IVWM compared to the TSB medium. Additionally, while EOs demonstrated overall significant anti-staphylococcal activity, their efficacy varied depending on the medium used. Generally, EOs displayed lower antimicrobial activity (against planktonic cells) in the IVWM than in the TSB medium. Interestingly, T-EO caused a higher reduction of biofilm cells in IVWM than in the TSB medium, in contrast to R-EO. Our findings suggest that EOs hold promise as agents for the treatment of biofilm-related wound infections. However, it is crucial to applyin vitroconditions that closely reflect the wound infection site to gain an accurate insight into the real-world activity of these antimicrobial/antibiofilm agents.
Biomarkers to predict steroid resistance in idiopathic nephrotic syndrome: a systematic review
Authors: May Carl J , Ford Nathan P 
Published: June 2023

AbstractIn this systematic review we have sought to summarise the current knowledge concerning biomarkers that can distinguish between steroid-resistant nephrotic syndrome and steroid-sensitive nephrotic syndrome. Additionally, we aim to select biomarkers that have the best evidence-base and should be prioritised for further research.Pub med and web of science databases were searched using “steroid resistant nephrotic syndrome AND biomarker”. Papers published between 01/01/2012 and 10/05/2022 were included. Papers that did not compare steroid resistant and steroid sensitive nephrotic syndrome, did not report sensitivity/specificity or area under curve and reviews/letters were excluded. The selected papers were then assessed for bias using the QUADAS-2 tool. The source of the biomarker, cut off, sensitivity/specificity, area under curve and sample size were all extracted. Quality assessment was performed using the BIOCROSS tool.17 studies were included, comprising 15 case-control studies and 2 cross-sectional studies. Given the rarity of nephrotic syndrome and difficulty in recruiting large cohorts, case-control studies were accepted despite their limitations.Haptoglobin and suPAR were identified as the most promising biomarkers based on their ability to predict rather than assess steroid resistance in nephrotic syndrome, their respective sample sizes and specificity and sensitivity.None of the selected papers stated whether the authors were blinded to the patient’s disease when assessing the index test in the cohort.These candidate biomarkers must now be tested with much larger sample sizes. Using new biobanks such as the one built by the NURTuRE-INS team will be very helpful in this regard.
A Sex specific homologue of snake Waprin is essential for Embryonic Development in the Red Flour Beetle,Tribolium castaneum
Authors: Choudhary Chhavi , Kishore Divyanshu , Meghwanshi Keshav Kumar , Verma Vivek , Shukla Jayendra Nath 
Published: June 2023

1.AbstractWaprin, an extracellular secretory protein, is widely known for its antibacterial activity. Through the analysis of sex-specific transcriptomes of adultTribolium castaneum, we identified a homologue of snakewaprin(Tc_WapF) with significantly higher level of its transcripts in females. Developmental- and tissue-specific profiling revealed the widespread expression ofTc_WapFin adult female tissues. Interestingly,Tc_WapFexpression is not regulated by the classical sex-determination cascade ofT. castaneum, as we fail to get any attenuation inTc_WapFtranscript levels inTcdsxandTctra(key players of sex determination cascade ofT. castaneum) knockdown females. The eggs laid byTc_WapFknockdown females never hatched, indicating the involvement ofTc_WapFin the embryonic development inT. castaneum. This is the first report of identification a sex-specificwaprinhomologue in an insect and its involvement as a novel candidate in the embryonic development. Future investigations on the functional conservation of insectwaprinsand their mechanistic role in embryonic development can be exploited for improving pest management strategies.Summary statementTribolium castaneumpossesses a female-specific homologue of snake Waprin (Tc_WapF) which is essential for its embryonic development. Expression ofTc_WapFis independent ofTriboliumsex-determination cascade.
Digital media and creative economy potential on youth employment in Kenya: a grounded theory perspective
Authors: Muchira John Munyui 
Published: June 2023

Purpose
Kenya leads East Africa in creative goods export and enjoys high internet penetration. Therefore, identifying pathways, missed opportunities for accelerating job creation and development in the sector and strategies for mitigating youth-related challenges are essential. This paper therefore aims to examine the effects of digital media and the cultural and creative industry (CCI) on youth employment and economic development.

Design/methodology/approach
The document review and analysis data came from 45 scientific and 23 grey literature articles. While the lack of primary data is a study limitation, secondary data were drawn from the United Nations Conference on Trade and Development Statistics and the 2016–2022 entertainment and media outlook analysis. Microsoft Excel 2021 for Windows was used to analyze quantitative data and generate results.

Findings
Since the CCI sector is characterized by limited financial opportunities, low youth participation in the labor force and weak policy frameworks, success in the industry requires key pathways. These include investment in digital-led innovations, developing innovative models, building partnerships with the private sector, strategic investment by government in the potential creative industry sub-sectors and identifying successful scaling-up models.

Originality/value
Information gathered through this study is crucial to counter the youth unemployment challenge and strategy identification, which could be used in skills and capabilities development in the potential creative economy. Future researchers must explore how to apply the proposed creative capacity theoretical lens to inform research in the sector.
Developing future managers through business simulation gaming in the UK and Hong Kong: exploring the interplay between cognitive realism, decision-making and performance
Authors: Lean Jonathan , Newbery Robert , Moizer Jonathan , Haddoud Mohamed , Lim Wai Mun 
Published: June 2023

PurposeThis paper investigates how individuals' decision-making approach and perceptions of a game's cognitive realism affect the performance of virtual businesses in a web-based simulation game.Design/methodology/approachSurvey data are collected from 274 business simulation game users and is analysed using the fsQCA technique.FindingsThe study identifies three alternative pathways to high and low performance in a business simulation game. Results indicate that a flexible decision-making approach exists in all high performance pathway solutions. Where a game is perceived to be realistic, a more focused decision-making approach is associated with high performance. However, where perceived cognitive realism is absent, a less focused experimental decision-making approach is employed, which increases the chances to achieve low performance. Finally, perceived cognitive realism and an experimental decision-making approach are found to be mutually exclusive for achieving high performance.Originality/valueWhilst the learning benefits of web-based simulation games are widely acknowledged, the complex interplay amongst factors affecting performance in games is under-researched. Limited research exists on how perceptions of a game's cognitive realism interact with user decision-making approaches to affect performance.
Improving startup’s attractiveness as industrial customers by organizing theirpurchasing activities
Authors: Tessaro Juliano Afonso , Harms Rainer , Schiele Holger 
Published: June 2023

Purpose
This study aims to analyze how startups organize their purchasing activities to improve operative excellence and become attractive customers.

Design/methodology/approach
The authors use a two-phase exploratory approach with semistructured interviews and a World Café. In total, 20 startup purchasers and suppliers participated. It is an international study with participants from eight countries (Belgium, Brazil, France, Germany, Hungary, The Netherlands, the UK and the USA).

Findings
The authors find that startups organize the purchasing function in five ways: partial outsourcing, transactional-oriented, strategic only, outsourced purchasing and full department. Each type has advantages and disadvantages regarding operative excellence. The authors identify type-specific antecedents to operative excellence: forecasting, payment habits, ordering process, contact accessibility and quick decision-making.

Research limitations/implications
The value of this paper is that it offers entrepreneurs a framework to organize startup purchasing activities, including outsourcing options. Furthermore, it provides theoretical contributions that expand the topic of purchasing and supply organization and operative excellence to the startup context.

Originality/value
The value of this paper is that, to the best of the authors’ knowledge, it is the first to explore purchasing organization and operative excellence in startups.
To be (family) or not to be (family): the familiness effect over brand authenticity posts on social media and consumer engagement relationship
Authors: Bargoni Augusto , Ballerini Jacopo , Vrontis Demetris , Ferraris Alberto 
Published: June 2023

Purpose
This paper aims to explore the impact of brand authenticity dimensions (i.e. aesthetic, symbolism, heritage, originality, quality commitment and virtue) on consumer engagement in the context of social media. This study answers to the need of scholars to understand consumer behaviour towards family and non-family firms’ brand authenticity constructs and for practitioners to find the correct levers to increase consumer engagement.

Design/methodology/approach
Top 10 European family firms with a retrievable Facebook (FB) page from the Global Family Business Index have been selected. Then, the study analysed family firms’ social media consumer engagement versus their non-family business direct competitors on a sample of 21.664 FB posts over a four-year period, leveraging multi-group analysis.

Findings
The results outline that three out of six brand authenticity dimensions posted on FB are statistically arousing more interactions respect to non-authenticity-related contents when posted by family firms. However, there are no statistically significant findings when brand authenticity content is posted by the non-family competitors.

Practical implications
This research is helpful for practitioners and entrepreneurs who might want to strengthen their social media brand strategies. With this regard, the study provides insights on which elements of brand authenticity are perceived by consumers as more engaging and which levers to use when communicating the familiness of the company.

Originality/value
To the best of authors’ knowledge, this is one of the earliest studies crosscutting the family business and brand authenticity literature streams to conduct an empirical analysis based on official FB data with a data set of over 20,000 observations. Moreover, this study assesses that not every dimension of the brand authenticity construct is relevant in the context of social media and that its effectiveness depends on the firms’ familiness.
A retrospective comparison between digital to conventional drainage systems for secondary spontaneous pneumothorax related to diffuse interstitial lung disease
Authors: Shikano Kohei , Abe Mitsuhiro , Hirama Ryutaro , Kitahara Shinsuke , Maruyama Kanae , Horiuchi Dai , Sakuma Noriko , Ishii Daisuke , Kawasaki Takeshi , Nakamura Hidenori , Suzuki Takuji 
Published: June 2023

AbstractIntroductionSecondary spontaneous pneumothorax (SSP) occurs as one of the complications associated with interstitial pneumonia (IP). Chest drainage is performed when there is a large volume of air in the pleural space. Notably, SSP with IP (SSP‐IP) is frequently not curable by chest drainage only. A digital drainage system (DDS) provides an objective evaluation of air leakage and maintains a pre‐determined negative pressure, compared to an analog drainage system (ADS). Few studies have reported the effectiveness of DDS in the treatment of SSP‐IP. This study aimed to assess the usefulness of DDS for SSP‐IP.MethodsThis retrospective study included patients with SSP‐IP who had undergone chest drainage. We reviewed the included patients' medical records, laboratory data, computed tomography findings, and pulmonary function data.ResultsDDS was used in 24 patients and ADS in 49 patients. The mean duration of chest drainage was 11.4 ± 1.9 days in the DDS group and 14.2 ± 1.3 days in the ADS group, which was not significantly different (p = 0.218). Surgery, pleurodesis, and/or factor XIII administration were performed in 40 patients. Additionally, five (20.8%) patients in the DDS group and nine (18.4%) in the ADS group had a recurrence of pneumothorax within 4 weeks (p = 1.000). One patient (14%) in the DDS group and six (12.2%) in the ADS group (p = 0.414) were cured of pneumothorax but later died.ConclusionDDS did not demonstrate a significant difference in the shortening of chest drainage duration. Further study is needed to validate the results of this study.
Novel insights into orchid mycorrhiza functioning from stable isotope signatures of fungal pelotons
Authors: Zahn Franziska E. , Söll Erik , Chapin Thomas K. , Wang Deyi , Gomes Sofia I. F. , Hynson Nicole A. , Pausch Johanna , Gebauer Gerhard 
Published: June 2023

Summary
Stable isotope signatures of fungal sporocarps have been instrumental in identifying carbon gains of chlorophyllous orchids from a fungal source. Yet, not all mycorrhizal fungi produce macroscopic sporocarps and frequently fungi of different taxa occur in parallel in orchid roots.
To overcome this obstacle, we investigated stable isotope signatures of fungal pelotons extracted from orchid roots and compared these data to the respective orchid and reference plant tissues. Anoectochilus sandvicensis and Epipactis palustris represented specialized or unspecialized rhizoctonia‐associated orchids. Epipactis atrorubens and Epipactis leptochila are orchids considered ectomycorrhiza‐associated with different preferences for Basidio‐ and Ascomycota.
13C enrichment of rhizoctonia pelotons was minor compared with plant tissues and significantly lower than enrichments of pelotons from ectomycorrhizal Epipactis species. 15N values of pelotons from E. leptochila and E. atrorubens showed similar patterns as known for respective sporocarps of ectomycorrhizal Ascomycota and Basidiomycota, however, with an offset towards lower 15N enrichments and nitrogen concentrations.
Our results suggest an explicit fungal nutrition source of orchids associated with ectomycorrhizal fungi, whereas the low 13C enrichment in rhizoctonia‐associated orchids and fungal pelotons hamper the detection of carbon gains from fungal partners. 15N isotopic pattern of orchids further suggests a selective transfer of 15N‐enriched protein‐nitrogen into orchids.
Emotion in the mind's eye: Imagination for adaptive cognition
Authors: Cocquyt Chantelle M. , Palombo Daniela J. 
Published: June 2023

AbstractIn a complex world, we are constantly faced with environmental stimuli that shape our moment‐to‐moment experiences. But just as rich and complex as the external world is the internal milieu—our imagination. Imagination offers a powerful vehicle for playing out hypothetical experiences in the mind's eye. It allows us to mentally time travel to behold what the future might bring, including our greatest desires or fears. Indeed, imagined experiences tend to be emotion‐laden. How and why are humans capable of this remarkable feat? Based on psychological findings, we highlight the importance of imagination for emotional aspects of cognition and behavior, namely in the generation and regulation of emotions. Based on recent cognitive neuroscience work, we identify putative neural networks that are most critical for emotional imagination, with a major focus on the default mode network. Finally, we briefly highlight the possible functional implications of individual differences in imagination. Overall, we hope to address why humans have the capacity to simulate hypothetical emotional experiences and how this ability can be harnessed in adaptive (and sometimes maladaptive) ways. We end by discussing open questions.
Did Dissociative Amnesia Evolve?
Authors: Patihis Lawrence 
Published: June 2023

AbstractDissociative amnesia is a diagnosis category that implies a proposed mechanism (often called dissociation) by which amnesia is caused by psychogenic means, such as trauma, and that amnesia is reversible later. Dissociative amnesia is listed in some of the most influential diagnostic manuals. Authors have noted the similarities in definition to repressed memories. Dissociative amnesia is a disputed category and phenomenon, and here I discuss the plausibility that this cognitive mechanism evolved. I discuss some general conditions by which cognitive functions will evolve, that is, the relatively continuous adaptive pressure by which a cognitive ability would clearly be adaptive if variation produced it. I discuss how adaptive gene mutations typically spread from one individual to the whole species. The article also discusses a few hypothetical scenarios and several types of trauma, to examine the likely adaptive benefits of blocking out memories of trauma, or not. I conclude that it is unlikely that dissociative amnesia evolved, and invite further development of these ideas and scenarios by others.
Development of a Portable Low-Cost System for the Metrological Verification of Wheelchair Roller Ergometers
Authors: Lancini Matteo , Spada Paolo , Muhametaj Ridi , Klerk Rick de , van der Woude Lucas H. V. , Vegter Riemer J. K. 
Published: June 2023

AbstractWheelchair ergometers are widely used in research, clinical practice, and sports environments. The majority of wheelchair ergometers are roller systems that allow for wheelchair propulsion in the personal wheelchair on one or two (instrumented) rollers. Oftentimes these systems are only statically calibrated. However, wheelchair propulsion is dynamic by nature, requiring a dynamic validation process. The aim of the current project was to present a low-cost portable system for the dynamic metrological verification of wheelchair roller ergometers, based on an instrumented reference wheel. The tangential force on the roller is determined, along with its uncertainty, from the reference wheel properties, and compared with the force measured by the ergometer. Uncertainty of this reference wheel system was found to be lower than the one of the ergometer used, indicating that this novel approach can be used for the metrological verification of ergometers.
Control-Oriented Reduced-Order Modeling of Conversion Efficiency in Dual-Layer Washcoat Catalysts With Accumulation and Oxidation Functions
Authors: Piqueras Pedro , Pla Benjamín , Sanchis Enrique José , García Elena 
Published: June 2023

Abstract
This work proposes a model for predicting conversion efficiency in multifunctional catalysts with dual-layer washcoat. The mass transfer is more relevant in these devices than in single-layer washcoats due to additional transport steps between the catalytic layers. In addition, the different reaction mechanisms between layers make the concentration of the chemical species differ in each layer. To deal with this boundary while considering the need for real-time computation, a reduced-order explicit solver for the convective diffusive reactive transport is presented for the case of dual-layer washcoats. Assuming one-dimensional quasi-steady flow, the solution procedure consisted of substituting the diffusive interfacial fluxes in the bulk gas and washcoat conservation equations by expressions that depend explicitly on the average concentration in the gas phase. The solution was then applied to model the performance of dual-layer oxidation catalysts with reductant accumulation in one washcoat layer, such as diesel oxidation catalyst (DOC) and ammonia slip catalyst (ASC) systems, during driving cycles. First, the response of these catalysts was analyzed by comparing them against experimental data and considering additional parameters provided by the model. Next, the importance of the mass transfer limitations was discussed to complete the analysis. The proposed model was compared with a simplified solver where the mass transfer steps were omitted, thus deteriorating the prediction capabilities in some driving cycle phases. Finally, a sensitivity study was performed to assess the impact of the mesh size on the prediction capabilities and computational requirements.