bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biogeophysical Context and Land Use and Coverage – Human Interventions in the Sado Estuarine Municipalities (Portugal)
Authors: Alves Neise Mare Souza , Silva Débora Barbosa da , Inácio Miguel , Cunha Ana Graça , Pimentel Nuno , Freitas Maria da Conceição 
Published: December 2022

Abstract
Anthropogenic modifications are changes in the landscape resulting from interventions on natural elements, usually related to the types of land use and coverage. This article analyzes the main biophysical components of the estuarine municipalities of the Sado River - emphasizing geology, geomorphology and anthropogenic derivations of the landscape. The systemic principles are the theoretical-methodological support of this integrated analysis. Cartographic materials, remote sensing techniques, Geographic Information Systems (GIS) and fieldwork were used. The components most impacted by anthropogenic interventions are: hydrography - with dams in river channels, which alter the flow dynamics and hydrosedimentary processes, favoring the occurrence of erosion in the tidal plain and siltation in the estuary, which requires dredging to maintain activity in the port of Setúbal; vegetation, soils, geology and geomorphology - with mining, which directly implies the suppression of vegetation and soils, the dismantling of relief and rocks, reduces biodiversity, characterizes the landscape, and is a potential risk of soil and water contamination. In the tidal plain originally colonized by salt marshes, there are significant economic activities: rice farming, salt culture, aquaculture and industrial area. Touristic developments are installed on the recent features of the coastal plain of the Tróia peninsula. These anthropogenic derivations affect the systemic relationships of natural elements and cause changes in the landscape. This integrated analysis can contribute to better planning and management of activities with environmental and social sustainability for the municipalities in the study area.
Porous hollow cobalt-based oxides encapsulated with bimetallic PdAu Nanoparticles of electrochemical biosensor for highly sensitive pesticides detection
Authors: Zhao Yaxu , Liang Lijun , Guo Wei , Lu Xiong , Zhao Congyi , Gao Faming 
Published: December 2022

Abstract
Efficient and portable electrochemical biosensors are received to evaluation of pesticides in the environment, which can make great significance for food safety. In this study, the Co-based oxides with a kind of hierarchical porous hollow and nanocages were constructed, in which the materials (Co3O4-NC) were encapsulated with PdAu Nanoparticles (NPs). Due to the unique porous structure, the changeable valence state of cobalt and the synergistic effect of bimetallic PdAu NPs, PdAu@Co3O4-NC possessed excellent electron pathways, and showed more exposed active sites. Accordingly, the porous Co-based oxides have been applied to construct an activeacetylcholinesterase (AChE) electrochemical biosensor, which showed good performance for organophosphorus pesticides (OPs) detection. The optimum AChE-CS/PdAu@Co3O4-NC/GCE platform allows to exhibit highly sensitive determination of omethoate and chlorpyrifos, with the relative low detection limit of 6.125 × 10-15 M and 5.10 × 10-13 M, respectively. And a wide linear range of 6.125 × 10−15 ~ 6.125 × 10−6 M and 5.10 × 10−13 ~ 5.10 × 10−6 M for these two pesticides were also achieved. Therefore, the PdAu@Co3O4-NC may represent a powerful tool for ultrasensitive sensing of (OPs), and have great potential application.
Clonorchis sinensis granulin promotes malignant transformation of human biliary epithelial cells by interacting with M2 macrophages via regulating STAT3 phosphorylation and MEK/ERK pathway
Authors: He Qing , Pan Xiaowen , Yin Yingxuan , Xu Anyuan , Wu Yinjuan , Li Xuerong , Yi Xueqing 
Published: December 2022

Abstract
Objective
Clonorchis sinensisgranulin (CsGRN) is a multifunctional growth factor that can promote the progression of cholangiocarcinoma (CCA). Here, we investigated the effect of CsGRN on human biliary epithelial cells (HIBECs) malignant transformation and its possible underlying mechanism.
Methods
HIBECs were treated with 10μg/ml of CsGRN recombinant proteins and 20 μg CsGRN recombinant plasmid was intravenously injected into each BALB/c wild-type mice. EdU-488 staining, colony formation assay, wound-healing assay, transwell assay, Western-blot, q-PCR, Immunohistochemistry staining, Immunofluorescence, H&E staining. flow cytometry and ELISA assay were carried out.
Results
CsGRN promoted excessive hyperplasia and abnormal metastasis of HIBECs by enhanced secretion of hepatic pro-inflammatory cytokines and chemokines as well as biliary injuries. In addition, CsGRN induced the M2-type polarization in macrophages both in vitro and in vivo. In addition, high expression of IL-6 was observed in the CsGRN treated co-cultured mediums, which activated the phosphorylation of STAT3, JAK2, MEK and ERK.
Conclusion
CsGRN induced the M2-type polarization of macrophages which further mediate the inflammation and malignant transformation of HIBECs by mediating the interactions between HIBECs and macrophages via IL-6/JAK2/STAT3 and MEK/ERK pathways.
XFEM implementation procedure for models involving contribution on interfaces
Authors: Estévez Peregrina Quintela , Rial Teresa Cao , González Carlos Moreno 
Published: December 2022

Abstract
Models involving interfaces with discontinuities or even singularities of some fieldsacross them are very frequent in real life problems modelling. The use of the eXtendedFinite Element Method (XFEM) instead of the traditional FEM has two advantages: themesh of the domain can be independent of the interface position, and it allows toenrich an area with specific shape functions fitted to singularities of the expectedsolution. Nevertheless, a critical point of its implementation is the different kind (andnumber) of degrees of freedom on each node. In this paper we present a detaileddescription of XFEM implementation, with special emphasis on the terms that involveintegration over interfaces. The methodology is presented in a general context, and asan example, we will apply it to a problem of solids mechanics. In particular, we willcontextualize the procedure on a Rayleigh waves propagation problem in a crackedstructure considering a Signorini contact condition on the crack sides.
Can deadwood be preferred to soil? Vascular plants on decaying logs in different forest types in Central Europe.
Authors: Unar Pavel , Daněk Pavel , Adam Dušan , Paločková Lenka , Holík Jan 
Published: December 2022

Abstract
Deadwood is known to be an irreplaceable substrate for various groups of organisms but its importance for vascular plants is still unclear. To examine which deadwood characteristics promote its colonization by vascular plants and whether species can prefer this substrate to mineral soil (or vice versa), we used regression modelling and ordination methods to analyse an extensive data set of vegetation records from more than 1800 pieces of lying deadwood and 200 control plots on soil. Data were collected in four old-growth forest sites ranging from lowland alluvial to mountain spruce forests. Colonization of deadwood of all decay classes, including freshly fallen logs, was frequent at all study sites. The density of colonizers differed between deadwood species, increased with deadwood decomposition stage and was higher when deadwood was exposed to light. On average, about 40% of observed species showed a preference for either deadwood or soil substrate with preference for soil usually being more common. Species that preferred deadwood to soil were typically early successional species, while those with preference for soil were often hygrophytes or typical understorey species. Most species responded consistently to substrate across multiple sites. With its unique microsite conditions and specific composition of plant colonizers, we highlight the importance of deadwood for herb layer species composition. Deadwood retention should be encouraged not only in protected but also in commercial forests.
Captivity restructures the gut microbiota of François’ langurs (Trachypithecus francoisi)
Authors: Mo Fengxiang , Li Yihui , Liu Zheng , Zheng Jingjin , Nong Dengpan , Liang Jipeng , Li Youbang , Huang Zhonghao 
Published: December 2022

AbstractData on gut microbiota of captive and wild animals can provide physiological and ecological basis for breeding and population rejuvenation of endangered species. Different diets lead to changes in the gut microbiota composition of animals. In this study, we examine the difference in the gut microbiota composition between the captive and wild Francois’ langurs (Trachypithecus francoisi), using 16S rRNA sequencing technology. The results showed that the composition of gut microbiota of captive langurs and wild langurs were characterized by Firmicutes (the captive langurs = 51.93% ± 10.07%; the wild langurs = 76.15% ± 8.37%) and Bacteroidetes (the captive langurs = 32.43% ± 10.00%; the wild langurs = 4.82% ± 1.41%) at the phylum level and were featured by Oscillospiraceae (the captive langurs = 15.80% ± 5.19%; the wild langurs = 30.21% ± 4.87%) at the family level. The alpha diversity of gut microbiota in captive langurs were higher than those in wild group, including Shannon index (4.45 ± 0.33 VS 3.98 ± 0.19, P < 0.001) and invSimpson index (35.11 ± 15.63 VS 19.02 ± 4.87, P < 0.001) but excluding Ace index (560.56 ± 62.44 VS 540.56 ± 39.72, P = 0.162;) and Chao index (570.86 ± 66.38 VS 544.78 ± 39.52, P = 0.141). PCoA result showed that there were significant differences in the composition of gut microbiota between captive and wild langurs at both phylum and family level (weight UniFrac algorithm, phylum level: R2 = 0.748, P = 0.001, family level: R2 = 0.685, P = 0.001). Captive langurs’ relative abundance of Firmicutes (51.93% ± 10.07%) was lower than that of wild langurs’ (76.15% ± 8.37%), and the relative abundance of Bacteroides (32.43% ± 10.00%) was higher than that of wild langurs’ (4.82% ± 1.41%). These differences should be linked to the fact that the diversity composition of captive langurs was lower than that of wild langurs and higher in simple carbohydrates. Variations in the gut microbiota of captive and wild should be linked to the differences in langurs’ dietary compositions, because the captive langurs consumed more fiber-rich and less simple carbohydrates-rich foods than wild langurs. Our study concluded that dietary composition could be crucial determinant in shaping gut microbiota of langurs, highlighting the importance of captivity on the gut microbiota structure and function and the need to consider the gut microbiota in animal provision.
Inverted resonance capture cascade:
modal interactions of a nonlinear energy sink with softening stiffness
Authors: Dekemele Kevin , Habib Giuseppe 
Published: December 2022

Abstract
Nonlinear energy sinks (NESs) are broadband passive vibration absorbers that are nonlinearly connected to a host system. If an NES is attached to a multi-degree-of-freedom mechanical host system under transient loading, the vibrations in the host system will transfer to and dissipate in the NES. During this transfer, the NES sequentially resonates with the modal frequencies of the host system, dissipating one mode at a time. This phenomenon is called resonance capture cascade (RCC). So far, RCC has only been investigated for NESs with a hardening nonlinear stiffness. Because of this stiffness, the transfer of modal vibrations happens from high to low frequency. In this study, an NES with a softening stiffness is proposed. Investigating the slow invariant manifolds reveals that an inverted resonance capture cascade occurs, where the transfer of vibrations to the NES is from low to high frequency. The analysis is carried out by exploiting high-dimensional slow invariant manifolds. The proposed NES is compared to the conventional NES with hardening stiffness.
High-content screening identifies a critical role for P pili in early adhesion of uropathogenic Escherichia coli to bladder cells
Authors: Simonet Thomas , Rutschmann Ophélie , Sharma Kunal , Nass Théo , Pavlou Maria , Dubois Anaëlle , Knott Graham , McKinney John 
Published: December 2022

Abstract
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are notoriously difficult to treat due to the ability of UPEC to adhere to and invade urothelial bladder cells. UPEC strains encode a variety of adhesins whose roles in adhesion and invasion are not fully elucidated. Using a transposon insertion library derived from the UPEC clinical isolate CFT073, we developed a high-content screening assay to identify UPEC mutants with defects in early adhesion to human bladder epithelial cells. Of a total of 8,184 mutants screened, we recovered 82 (1.0%) and 54 (0.7%) mutants with decreased and increased adhesion, respectively. Surprisingly, nine low-adhesion hits mapped to the two P pili operons encoded by CFT073, which are usually thought to mediate adhesion to kidney cells rather than bladder cells. These results were reinforced by examination of six high-adhesion hits mapping to the operon coding for F1C pili, where disruption of F1C pili function resulted in increased P pili synthesis. Taken together, these findings reveal a critical role for P pili in UPEC adhesion to bladder epithelial cells, which may inform the development of anti-adhesion therapies to prevent UTI recurrence.
Performance enhancement of UAV relay-assisted free-space optics systems with pointing errors
Authors: Elsayed Ebrahim E. , Hayal Mohammed R. , Kakati Dhiman , Singh Mehtab , Alfikky Abdelrahman A. , Boghdady Ayman I. , Grover Amit 
Published: December 2022

Abstract
This paper investigates and enhances unmanned aerial vehicle (UAV) relay-assisted free-space optics (FSO) communication systems under the effects of pointing errors (PEs) and atmospheric turbulences. The incorporation of UAVs as buffer-aided moving relays in the conventional FSO (CFSO) relay-assisted systems is proposed for enhancing the performance of PEs through AT. Using M-PSK (phase shift keying) and M-QAM (quadrature amplitude modulation), the impact of PEs on transmission quality is evaluated in this work. We examine and enhance the symbol error rate, outage probability (OP), and signal-to-noise ratio (SNR) for the UAV-to-ground stationbased FSO communications systems. The spatial diversity-based relay-assisted CFSO systems can enhance the performance of the UAV-UAV FSO links. Numerical results show that to achieve a bit-error-rate (BER) of ≤ 10-5 , the required SNR is ≥ 23 dB when the wind variance of the UAVs 𝜎𝛼2 increases from 0 to 7 mrad with FSO link distance L = 2000 m. The required SNR is ≥ 25 dB when the wind variance 𝜎𝛼2 is 1 mrad at a OP of 10-6 . To obtain an average BER of 10−6 , the SNR should be 16.23 dB, 17.64 dB, and 21.45 dB when 𝜎𝛼2 is 0 mrad, 1 mrad, and 2 mrad, respectively. Using 8-PSK modulation without PEs requires 23.5 dB at BER of 10-8 while 16-QAM without PEs requires 26.5 dB to maintain the same BER of 10-8 . Compared with 16-QAM without PEs, the SNR gain of 8-PSK without PEs is 3 dB.
Economic Strategy for the Impact of the COVID-19 Pandemic on Construction Project Implementation
Authors: Andiyan Andiyan 
Published: December 2022

Abstract
Aside from project delays, the COVID-19 pandemic's impact on the construction industry includes the distribution of construction supplies and materials, labor and labor reductions, work hour reductions, and even temporary work stoppage for projects in the red zone. Domestic construction, on the other hand, is in a pickle since PUPR Minister Basuki Hadimuljono said that despite the COVID-19 epidemic, construction services would continue to operate under the Instruction of the Minister of PUPR No 2/2020, which was issued on March 27, 2020. This forces the construction industry to continue operating while adapting to numerous adjustments during and after the epidemic. Method A survey is the data-gathering tool that will be utilized in this study. The data collection instrument will be a questionnaire that will be distributed either online or offline. Principal Component Analysis was employed as the analytical technique (Principal Component Analysis). The findings of this research indicate that the following elements contribute to the effect of the Covid-19 pandemic on construction projects: project finance factors, regional restraint factors (PSBB), labor factors, material and equipment factors, project uncertainty factors, and strike factors. Numerous ways may be used to undertake construction projects during and after a pandemic, each one tailored to the primary components generated. For the first Main component, the following solutions are possible: 1) Managing the cash flow of the project effectively and efficiently; 2) Using the way of analysis of the value of the outcomes (earn value method) to manage the project's budget and schedule; 3) Preparing an unexpected budget for the project.
Human Action Detection and Recognition: A Pragmatic Approach using Multiple Feature Extraction Techniques and Convolutional Neural Networks
Authors: Basavaiah Jagadeesh , Anthony Audre Arlene 
Published: December 2022

Abstract
Action recognition is described as the capability of determining the action that a human exhibit in the video. Latest innovations in either deep-learning or hand-crafted methods substantially increased the accuracy of action recognition. However, there are many issues, which keep action recognition task far from being solved. The task of human action recognition persists to be complicated and challenging due to the high complexity associated with human actions such as motion pattern variation, appearance variation, viewpoint variation, occlusions, background variation and camera motion. This paper presents a computational approach for human action recognition using video datasets through different stages: Detection, tracking of human and recognition of actions. Human detection and tracking are carried out using Gaussian Mixture Model (GMM) and Kalman filtering respectively. Different feature extraction techniques such as Scale Invariant Feature Transform (SIFT), Optical Flow Estimation, Bi-dimensional Empirical Mode Decomposition (BEMD), Discrete Wavelet Transform (DWT) are used to extract optimal features from the video frames. The features are fed to the Convolutional Neural Network classifier to recognize and classify the actions. Three datasets viz. KTH, Weizmann and Own created datasets are used to evaluate the performance of the developed method. Using SIFT, BEMD and DWT multiple feature extraction technique, the proposed method is called Hybrid Feature Extraction – Convolutional Neural Network based Video Action Recognition (HFE-CNN-VAR) method. The results of the work demonstrated that the HFE-CNN-VAR method enhanced the accuracy of action classification. The accuracy of classification is 99.33% for Weizmann dataset, 99.01% for KTH dataset and 90% for own dataset. Results of the experiment and comparative analysis shows that proposed approach surpasses when compared with other contemporary techniques.
The Impact of Firm-Specific and Macroeconomic Factors on the Financial Performance of Insurance Industry: Evidence from Ethiopia.
Authors: Girma Abebe Gule 
Published: December 2022

Abstract
In the current business environment, the financial performance of companies and its determinant factors are considered as a key issues. The purpose of this study was to examine the impact of firm-specific and macroeconomic factors on the financial performance of the insurance industry in Ethiopia, which covers a 10-year panel study from 2008 to 2017 based on data extracted from the financial statements of nine insurance companies and macroeconomic data from the World Bank data base. Data was collected from from secondary source only, descriptive research design used for this study, nine insurance company selected as a sample based on judmental sampling thechnique and employed the pooled OLS regression methoods to figure out the relationship between the explanatory variables and the financial performance of the insurance firm in ethiopia. The results obtained from the regression analysis shows that efficiency, size of insurance and liquidity have a statistically significant and positive effect on insurance financial performance, while firm leverage and the lending interest rate have a statistically negative significant effect on the financial performance of Ethiopian insurance companies. However, the impact of GDP and inflation is insignificant. Finally this study results recommends to insurance managers to give careful attention for those firm specific and macro-economic determinant variables to Improve the financial performance of the business.
SETD2 non genomic loss of function in advanced systemic mastocytosis is mediated by an Aurora kinase A/MDM2 axis and can be therapeutically targeted
Authors: Mancini Manuela , Monaldi Cecilia , Santis Sara , Papayannidis Cristina , Rondoni Michela , Sartor Chiara , Bruno Samantha , Pagano Livio , Criscuolo Marianna , Zanotti Roberta , Bonifacio Massimiliano , Tosi Patrizia , Arock Michel , Valent Peter , Cavo Michele , Soverini Simona 
Published: December 2022

Abstract
Background: The SETD2 tumor suppressor gene encodes a histone methyltransferase that safeguards transcription fidelity and genomic integrity via trimethylation of histone H3 lysine 36 (H3K36Me3). SETD2 loss of function has been observed in solid and hematologic malignancies. We have recently reported that most patients with advanced systemic mastocytosis (AdvSM) and some with indolent or smoldering SM display H3K36Me3 deficiency as a result of a reversible loss of SETD2 due to reduced protein stability.
Methods: Experiments were conducted in SETD2-proficient (ROSAKIT D816V) and -deficient (HMC-1) cell lines and in primary cells from patients with various SM subtypes. A short interfering RNA approach was used to silence SETD2 (in ROSAKIT D816V cells), MDM2 and AURKA (in HMC-1 cells). Protein expression and post-translational modifications was assessed by Western Blotting and Immunoblotting. Proteins interactions were tested by using co-immunoprecipitation. Apoptotic cell death was evaluated by flow cytometry after annexin V and propidium iodide staining, respectively. Drug cytotoxicity in in vitro experiments was evaluated by clonogenic assays.
Results: Here, we show that the proteasome inhibitors carfilzomib and ixazomib suppress cell growth and induce apoptosis in neoplastic mast cells by promoting SETD2/H3K36Me3 re-expression. Moreover, we found that Aurora kinase A and MDM2 are implicated in SETD2 loss of function in AdvSM. In line with this observation, direct or indirect targeting of Aurora kinase A with alisertib or volasertib induced reduction of clonogenic potential and apoptosis in human mast cell lines and primary neoplastic cells from patients with AdvSM. Efficacy of Aurora A or proteasome inhibitors was comparable to that of avapritinib.
Conclusions: Our mechanistic insights into SETD2 non-genomic loss of function in AdvSM highlight the potential value of novel therapeutic targets and agents for the treatment of patients who fail or do not tolerate midostaurin or avapritinib.