bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adjudicated myocarditis and multisystem illness trajectory in healthcare workers post-COVID-19
Authors: Sykes Robert , Morrow Andrew J , McConnachie Alex , Kamdar Anna , Bagot C , Bayes Hannah , Blyth Kevin G , Briscoe Michael , Bulluck Heeraj , Carrick David , Church Colin , Corcoran David , Delles C , Findlay Iain , Gibson Vivienne B , Gillespie Lynsey , Grieve Douglas , Barrientos Pauline Hall , Ho Antonia , Lang N N , Lowe David J , Lennie Vera , MacFarlane Peter , Mayne Kaithlin J , Mark Patrick , McIntosh Alasdair , McGeoch Ross , McGinley Christopher , Mckee Connor , Nordin Sabrina , Payne Alexander , Rankin Alastair , Robertson Keith E , Ryan Nicola , Roditi Giles H , Sattar Naveed , Stobo David B , Allwood-Spiers Sarah , Touyz Rhian , Veldtman Gruschen , Weeden Sarah , Watkins Stuart , Welsh Paul , Wereski Ryan , Mangion Kenneth , Berry Colin 
Published: February 2023

BackgroundWe investigated the associations of healthcare worker status with multisystem illness trajectory in hospitalised post-COVID-19 individuals.Methods and resultsOne hundred and sixty-eight patients were evaluated 28–60 days after the last episode of hospital care. Thirty-six (21%) were healthcare workers. Compared with non-healthcare workers, healthcare workers were of similar age (51.3 (8.7) years vs 55.0 (12.4) years; p=0.09) more often women (26 (72%) vs 48 (38%); p<0.01) and had lower 10-year cardiovascular risk (%) (8.1 (7.9) vs 15.0 (11.5); p<0.01) and Coronavirus Clinical Characterisation Consortium in-hospital mortality risk (7.3 (10.2) vs 12.7 (9.8); p<0.01). Healthcare worker status associated with less acute inflammation (peak C reactive protein 48 mg/L (IQR: 14–165) vs 112 mg/L (52–181)), milder illness reflected by WHO clinical severity score distribution (p=0.04) and shorter duration of admission (4 days (IQR: 2–6) vs 6 days (3–12)).In adjusted multivariate logistic regression analysis, healthcare worker status associated with a binary classification (probable/very likely vs not present/unlikely) of adjudicated myocarditis (OR: 2.99; 95% CI (1.01 to 8.89) by 28–60 days postdischarge).After a mean (SD, range) duration of follow-up after hospital discharge of 450 (88) days (range 290, 627 days), fewer healthcare workers died or were rehospitalised (1 (3%) vs 22 (17%); p=0.038) and secondary care referrals for post-COVID-19 syndrome were common (42%) and similar to non-healthcare workers (38%; p=0.934).ConclusionHealthcare worker status was independently associated with the likelihood of adjudicated myocarditis, despite better antecedent health. Two in five healthcare workers had a secondary care referral for post-COVID-19 syndrome.Trial registration numberNCT04403607.
Cardiac abnormalities in Long COVID 1-year post-SARS-CoV-2 infection
Authors: Roca-Fernandez Adriana , Wamil Malgorzata , Telford Alison , Carapella Valentina , Borlotti Alessandra , Monteiro David , Thomaides-Brears Helena , Kelly Matt , Dennis Andrea , Banerjee Rajarshi , Robson Matthew , Brady Michael , Lip Gregory Y H , Bull Sacha , Heightman Melissa , Ntusi Ntobeko , Banerjee Amitava 
Published: February 2023

BackgroundLong COVID is associated with multiple symptoms and impairment in multiple organs. Cross-sectional studies have reported cardiac impairment to varying degrees by varying methodologies. Using cardiac MR (CMR), we investigated a 12-month trajectory of abnormalities in Long COVID.ObjectivesTo investigate cardiac abnormalities 1-year post-SARS-CoV-2 infection.Methods534 individuals with Long COVID underwent CMR (T1/T2 mapping, cardiac mass, volumes, function and strain) and multiorgan MRI at 6 months (IQR 4.3–7.3) since first post-COVID-19 symptoms. 330 were rescanned at 12.6 (IQR 11.4–14.2) months if abnormal baseline findings were reported. Symptoms, questionnaires and blood samples were collected at both time points. CMR abnormalities were defined as ≥1 of low left or right ventricular ejection fraction (LVEF), high left or right ventricular end diastolic volume, low 3D left ventricular global longitudinal strain (GLS), or elevated native T1 in ≥3 cardiac segments. Significant change over time was reported by comparison with 92 healthy controls.ResultsTechnical success of multiorgan and CMR assessment in non-acute settings was 99.1% and 99.6% at baseline, and 98.3% and 98.8% at follow-up. Of individuals with Long COVID, 102/534 (19%) had CMR abnormalities at baseline; 71/102 had complete paired data at 12 months. Of those, 58% presented with ongoing CMR abnormalities at 12 months. High sensitivity cardiac troponin I and B-type natriuretic peptide were not predictive of CMR findings, symptoms or clinical outcomes. At baseline, low LVEF was associated with persistent CMR abnormality, abnormal GLS associated with low quality of life and abnormal T1 in at least three segments was associated with better clinical outcomes at 12 months.ConclusionCMR abnormalities (left entricular or right ventricular dysfunction/dilatation and/or abnormal T1mapping), occurred in one in five individuals with Long COVID at 6 months, persisting in over half of those at 12 months. Cardiac-related blood biomarkers could not identify CMR abnormalities in Long COVID.Trial registration numberNCT04369807.
Effect of COVID-19 pandemic restrictions on chlamydia and gonorrhoea notifications and testing in Queensland, Australia: an interrupted time series analysis
Authors: Dalmau Marguerite , Ware Robert , Field Emma , Sanguineti Emma , Si Damin , Lambert Stephen 
Published: February 2023

ObjectiveTo investigate trends in testing and notifications of chlamydia and gonorrhoea during the COVID-19 pandemic in Queensland, Australia.MethodsStatewide disease notification and testing data between 1 January 2015 and 31 December 2021 were modelled using interrupted time series. A segmented regression model estimated the pre-pandemic trend and observed effect of the COVID-19 pandemic response on weekly chlamydia notifications, monthly gonorrhoea notifications and monthly testing figures. The intervention time point was 29 March 2020, when key COVID-19 public health restrictions were introduced.ResultsThere were 158 064 chlamydia and 33 404 gonorrhoea notifications and 2 107 057 combined chlamydia and gonorrhoea tests across the 72-month study period. All three studied outcomes were increasing prior to the COVID-19 pandemic. Immediate declines were observed for all studied outcomes. Directly after COVID-19 restrictions were introduced, declines were observed for all chlamydia notifications (mean decrease 48.4 notifications/week, 95% CI −77.1 to –19.6), gonorrhoea notifications among males (mean decrease 39.1 notifications/month, 95% CI −73.9 to –4.3) and combined testing (mean decrease 4262 tests/month, 95% CI −6646 to –1877). The immediate decline was more pronounced among males for both conditions. By the end of the study period, only monthly gonorrhoea notifications showed a continuing decline (mean decrease 3.3 notifications/month, p<0.001).ConclusionThere is a difference between the immediate and sustained impact of the COVID-19 pandemic on reported chlamydia and gonorrhoea notifications and testing in Queensland, Australia. This prompts considerations for disease surveillance and management in future pandemics. Possible explanations for our findings are an interruption or change to healthcare services during the pandemic, reduced or changed sexual practices or changed disease transmission patterns due to international travel restrictions. As pandemic priorities shift, STIs remain an important public health priority to be addressed.
Microparticle-Delivered Cxcl9 Prolongs Braf Inhibitor Efficacy in Melanoma
Authors: Romano Gabriele , Paradiso Francesca , Li Peng , Shukla Pooja , Barger Lindsay N. , El Naggar Olivia , Miller John P. , Liang Roger J. , Helms Timothy L. , Lazar Alexander J. , Wargo Jennifer A. , Taraballi Francesca , Costello James C. , Kwong Lawrence N. 
Published: February 2023

Abstract
Patients with BRAF-mutant melanoma show substantial responses to combined BRAF and MEK inhibition, but most relapse within 2 years. A major reservoir for drug resistance is minimal residual disease (MRD), comprised of drug-tolerant tumor cells laying in a dormant state. Towards exploiting potential therapeutic vulnerabilities of MRD, we established a genetically engineered mouse model of BrafV600E-driven melanoma MRD wherein genetic BrafV600E extinction leads to strong but incomplete tumor regression. Transcriptional time-course analysis after BrafV600E extinction revealed that after an initial surge of immune activation, tumors later became immunologically “cold” after MRD establishment. Computational analysis identified candidate T-cell recruiting chemokines as strongly upregulated initially and steeply decreasing as the immune response faded. Therefore, we hypothesized that sustaining chemokine signaling could impair MRD maintenance through increased recruitment of effector T cells. We found that intratumoral administration of recombinant Cxcl9 (rCxcl9), either naked or loaded in microparticles, significantly impaired MRD relapse in BRAF-inhibited tumors, including several complete pathologic responses after microparticle-delivered rCxcl9 combined with BRAF and MEK inhibition. Our experiments constitute proof of concept that chemokine-based microparticle delivery systems are a potential strategy to forestall tumor relapse and thus improve the clinical success of first-line treatment methods.
Spatiotemporal Variabilities of the Recent Indian Summer Monsoon Activities in the Tibetan Plateau: A Reanalysis of Outgoing Longwave Radiation Datasets
Authors: Guo Xiaoyu , Tian Lide , Wang Lei , Zhang Lin 
Published: February 2023

Abstract
Unstable hydrological cycles and water resource instability over and around the Tibetan Plateau (TP) are topics of wide concern. The Indian summer monsoon (ISM) is one of the TP’s most important moisture sources; as such, its behavior is key to any changes in precipitation and water-related environments. However, there have been relatively few thorough investigations into ISM activities. Here we primarily explore ISM activities using outgoing longwave radiation (OLR) datasets in TP, and precipitation isotopes recorded at Lhasa, for the period 1975–2020. Our major findings are that 1) the ISM onset (retreat date) is between ∼31 May and 19 July (∼8 August–27 September), with ISM duration of ∼40–110 days; 2) significant spatial inhomogeneous patterns are evident in ISM activities, i.e., the western part of our study area experiences earlier ISM onset, delayed retreat, longer duration, and greater intensity and strength, and the inverse is true in the eastern sector of the study area; 3) the ISM activities that dominate the 1975–98 period determine their general patterns over the entire 1975–2020 period, taking into account evident discrepancies in subperiods; and 4) the negative relations between precipitation δ18O and ISM intensity/strength at Lhasa confirm the ISM activities defined using OLR. These results will improve our understanding of hydrological cycles in TP and provide insights into hydrological studies in the “Asian Water Tower” region.

Significance Statement
Over the recent decades, the Tibetan Plateau (TP) (Asian Water Tower) has undergone dramatic environmental changes, evinced by the instabilities of hydrological cycles. As one of TP’s most important moisture sources, the Indian summer monsoon (ISM) is key to changes in precipitation and water-related environments. To get thorough investigations into ISM activities, we primarily explore ISM activities using outgoing longwave radiation (OLR) datasets in TP and precipitation isotopes at Lhasa. Significant spatial inhomogeneous patterns are evident in ISM activities: the western part experiences earlier ISM onset, delayed retreat, longer duration, and stronger intensity and strength, and the inverse occurs in the eastern sector. These results will improve our understanding of hydrology, meteorology, ecology, and paleoclimate reconstructions in the Asian Water Tower region.
Validation of computational fluid dynamics simulations for determining pressure loss coefficients of ventilation components
Authors: Tawackolian Karsten , Kriegel Martin 
Published: February 2023

Ventilation systems include a variety of components for which necessary pressure loss data is often unavailable. Computational fluid dynamics simulations could substitute for expensive measurements, but validation simulations with suitable data are crucial to assess model uncertainties. Existing CFD validation studies either did not focus specifically on pressure losses, only covered few components, or did not include recent developments in turbulence modelling. In the present work, 33 bends, 4 gates and 2 tees were simulated using a consistent approach. Computational fluid dynamics simulations were validated with published data: rectangular high-edge and wide-edge bends from the experimental dataset of Sprenger, gates and diverging tees from the SMACMA guide. The considered flows cover important basic flow phenomena: deflection, splitting and flow separation. The 39 components were simulated with three turbulence models at 14 Reynolds numbers. The simulations predicted pressure loss coefficients accurately for various components. Cases with strong flow separation regions were most challenging. The model prediction uncertainty was assessed by carrying out simulations with three selected turbulence models. As in the experimental data from Sprenger, the simulations showed a distinct dependence of pressure loss coefficients on the Reynolds number for bends. In contrast, for abrupt deflections and flow separation at sharp edges, the Reynolds number dependency was minor. Technical pressure loss data of ductwork components is needed for the dimensioning, optimisation, and energy assessment of ventilation systems. The present validation study assesses the present state of the art of CFD simulations to determine pressure loss coefficients and the resulting prediction uncertainties.
Emergency unit assessment of seven tertiary hospitals in Nepal using the WHO tool: a cross-sectional study
Authors: Kharel Ramu , Thapa Ghan B. , Voor Tamara , Pant Samriddha R. , Adhikari Samir K. , Bist Bimal S. , Relan Pryanka , Lin Timmy , Lubetkin Derek , Deluca Giovanna , Shilpakar Olita , Shrestha Sanu K. , Pokharel Yagya R. , Paudel Santosh , Thapa Ajay S. , Shakya Yogendra M. , Karki Achyut R. , Dhakal Nishant , Aluisio Adam R. 
Published: February 2023

Abstract
Background
In 2021, the Nepal national emergency care system’s assessment (ECSA) identified 39 activities and 11 facility-specific goals to improve care. To support implementation of the ECSA facility-based goals, this pilot study used the World Health Organization’s (WHO) Hospital Emergency Unit Assessment Tool (HEAT) to evaluate key functions of emergency care at tertiary hospitals in Kathmandu, Nepal.

Methods
This cross-sectional study used the standardized HEAT assessment tool. Data on facility characteristics, human resources, clinical services, and signal functions were gathered via key informant interviews conducted by trained study personnel. Seven tertiary referral centers in the Kathmandu valley were selected for pilot evaluation including governmental, academic, and private hospitals. Descriptive statistics were generated, and comparative analyses were conducted.

Results
All facilities had continuous emergency care services but differed in the extent of availability of each item surveyed. Academic institutions had the highest rating with greater availability of consulting services and capacity to perform specific signal functions including breathing interventions and sepsis care. Private institutions had the highest infrastructure availability and diagnostic testing capacity. Across all facilities, common barriers included lack of training of key emergency procedures, written protocols, point-of-care testing, and ancillary patient services.

Conclusion
This pilot assessment demonstrates that the current emergency care capacity at representative tertiary referral hospitals in Kathmandu, Nepal is variable with some consistent barriers which preclude meeting the ECSA goals. The results can be used to inform emergency care development within Nepal and demonstrate that the WHO HEAT assessment is feasible and may be instructive in systematically advancing emergency care delivery at the national level if implemented more broadly.
Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China
Authors: Shao Keqiang , Zhang Lei , Ba Tunasheng , Chao Jianying , Gao Guang 
Published: February 2023

AbstractSediment bacterial communities play a critical role in biogeochemical cycling in alpine lake ecosystems. However, little is known about the sediment microbial communities in these lakes. In this study, the bacterial community composition (BCC) and their relationships with environmental factors of the sediment in Sayram Lake, the largest alpine and cold-water inland lake, China was analyzed using Illumina MiSeq sequencing. In total, we obtained 618,271 high quality sequences. The results showed that the bacterial communities with 30 phyla and 546 genera, were spread out among the 5 furface sediment samples, respectively. The communities were dominated by Proteobacteria, Acidobacteria, Planctomycetes, Gemmatimonadetes, Chloroflexi, Actinobacteria, Verrucomicrobia and Bacteroidetes, accounting for 48.15 ± 8.10%, 11.23 ± 3.10%, 8.42 ± 2.15%, 8.37 ± 2.26%, 7.40 ± 3.05%, 5.62 ± 1.25%, 4.18 ± 2.12% and 2.24 ± 1.10% of the total reads, respectively. At the genus level, the communities were dominated by Aquabacterium, Pseudomonas, Woeseia, MND1, Ignavibacterium and Truepera, accounting for 7.89% ± 8.24%, 2.32% ± 1.05%, 2.14% ± 0.94%, 2% ± 1.22%, 0.94% ± 0.14% and 0.80% ± 0.14% of the total reads, respectively. Statistical analyses showed the similarity of the sediment bacterial communities at our field sites was considerably low, far below 35%, and total organic carbon (TOC) was the dominant environmental factor affecting the spatial changes of BCC in the sediment. Thus, this study greatly improving our understanding of the microbial ecology of alpine lake in the arid and semi-arid ecosystems today so seriously threatened.
The biological and biochemical composition of wheat (Triticum aestivum) as affected by the bio and organic fertilizers
Authors: Lamlom Sobhi F. , Irshad Ahsan , Mosa Walid F. A. 
Published: February 2023

AbstractMicroorganisms and organic compounds (humic and fulvic acid) offer viable alternatives to insecticides and mineral fertilizers. Even though many studies have shown the effects of biofertilizers and organic substances separately, little information is available on plant responses to the combined application of these bio-stimulants, even though these biological inputs have a high potential for simultaneous action. A two-year (2020/21–2021/22) field experiment was conducted to investigate the impact of organic and biofertilizers application on the growth, yield, and biochemical attributes of wheat (cv. Misr-1). Pre-planting, wheat seeds were inoculated with two biofertilizers including Mycorrhizae, and Azotobacter, and their combination (MIX), and control (un-inoculation) were considered the main plot factor. The subplot factor contained the foliar sprays of humic acid, fulvic acid, and control (no spray). The results revealed that the seed inoculation with mycorrhizae and azotobacter in combination with foliar-applied humic acid markedly (p ≤ 0.05) affected the growth, yield, and seed biochemical composition of wheat. Combination of mycorrhiza and azotobacter significantly (p ≤ 0.05) increased) plant height (100 cm), crop growth rate (18.69 g), number of spikelets per spike (22), biological yield (13.4 ton ha-1), grain yield (5.56 ton ha-1), straw yield (8.21 ton ha-1),), nitrogen (2.07%), phosphorous (0.91%), potassium (1.64%), protein content (12.76%), starch (51.81%), and gluten content (30.90%) compared to control. Among organic fertilizers, humic acid caused the maximum increase in plant height (93 cm), crop growth rate ( 15 g day-1 m-2),1000 grain weight (51 g), biological yield ( 11ton ha-1), grain yield (4.5 ton ha-1), protein content (11%), chlorophyll content (46 SPAD), and gluten (29.45%) as compared to all other treatments. The foliar application of humic acid combined with the mycorrhizae or azotobacter seed inoculation was efficient to induce wheat vegetative growth development, as well as yield and its components.
Characterization and identification of the powdery mildew resistance gene in wheat breeding line ShiCG15–009
Authors: Zhang Wenjing , Yu Ziyang , Wang Dongmei , Xiao Luning , Su Fuyu , Mu Yanjun , Zheng Jianpeng , Li Linzhi , Yin Yan , Yu Tianying , Jin Yuli , Ma Pengtao 
Published: February 2023

AbstractPowdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a serious fungal disease that critically threatens the yield and quality of wheat. Utilization of host resistance is the most effective and economical method to control this disease. In our study, a wheat breeding line ShiCG15–009, released from Hebei Province, was highly resistant to powdery mildew at all stages. To dissect its genetic basis, ShiCG15–009 was crossed with the susceptible cultivar Yannong 21 to produce F1, F2 and F2:3 progenies. After genetic analysis, a single dominant gene, tentatively designated PmCG15–009, was proved to confer resistance to Bgt isolate E09. Further molecular markers analysis showed that PmCG15–009 was located on chromosome 2BL and flanked by markers XCINAU130 and XCINAU143 with the genetic distances 0.2 and 0.4 cM, respectively, corresponding to a physic interval of 705.14–723.48 Mb referred to the Chinese Spring reference genome sequence v2.1. PmCG15–009 was most likely a new gene differed from the documented Pm genes on chromosome 2BL since its different origin, genetic diversity, and physical position. To analyze and identify the candidate genes, six genes associated with disease resistance in the candidate interval were confirmed to be associated with PmCG15–009 via qRT-PCR analysis using the parents ShiCG15–009 and Yannong 21 and time-course analysis post-inoculation with Bgt isolate E09. To accelerate the transfer of PmCG15–009 using marker-assisted selection (MAS), 18 closely or co-segregated markers were evaluated and confirmed to be suitable for tracing PmCG15–009, when it was transferred into different wheat cultivars.