bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of BHMT2 gene expression and its expression factor regulating LNC00922 LNCRNA with rs10944 genotype determination in patients with breast cancer
Authors: Zavareh Seyed Ali Dibaj , Akhlaghi Zahra , Esfahani Sarina Samiei , Mohammadi Niloofar , Rahimirad Samira , Azadeh Mansoureh 
Published: August 2023

Abstract
Background: It is predicted that dysregulation of BHMT2 expression level could have a remarkable effect on Breast cancer status. The absence of methionine and Hyperhomocysteinemia have been linked to a decline in BHMT2 expression. This mRNA expression and its correlated long non- coding RNA (lncRNA) (LINC00922) have been investigated in our experiment based on the bioinformatics approach. Also, the genotype frequency analysis of BHMT2 in SNP rs10499 has been demonstrated. The mentioned SNP is correlated with the differences in the binding affinity of miR542-3p.
Material and method: we performed a microarray analysis to find the differentially expressed genes (DEGs). GeneMANIA and miRWalk online databases were used for mRNA-mRNA and mRNA-miRNA interaction analyses. Clusterprofiler package was used for functional and pathway enrichment analyses. dbSNP database was applied for finding the relevant Single nucleotide polymorphism
(SNP). The Co-lncRNA database was used for finding the correlated lncRNA.A real-time PCR experiment was performed to count the relative expression level of BHMT2 and LINC00922. The high resolution melt (HRM) method was performed to identify the SNP genotype frequency.
Result: BHMT2 had a significant down-regulation, and LINC00922 had a significant up-regulation in Breast cancer tissues compared to normal. No significant difference in genotype frequency of rs10944 was observed.
Conclusion: The current study revealed that BHMT2 and LINC00922 can be the two novel correlated biomarker of breast cancer. More similar investigations are needed for evaluating more accurate and reliable result, specially about rs10499.
Intention To Use Patient Portal And Its Predictors Among Diabetic Patients Enrolled In Referral Hospitals, Amhara Regional State, Northwest Ethiopia, 2023: UTAUT-2 Model.
Authors: Abebe Aschale Wubete , Tilahun  , Mekonnen( Zeleke Abebaw , (MPH) Masresha Derese Tegegne 
Published: August 2023

Abstract

Background:Patient portal technology is increasingly utilized in the health care system for diabetes management, as means of communication and information-sharing tools and it has the potential to improve access, quality, and outcomes for diabetic patients. However, its adaption is relatively low and patients’ intention towards patient portal is unknown. This study aims to fill this gap by determining the intention to use the patient portal and its predictors among diabetic patients in Ethiopia.Method:An institution-based cross-sectional study was conducted on 1078 diabetes patients from April 3 to May 8 in eight referral hospitals Amhara region, Ethiopia 2023. Sample was proportionally allocated for each hospital and participants were selected by using a systematic random sampling method. The data were collected in the same period in all hospitals by using interviewer-administered questioner; by kobo collect mobile app. Descriptive statistics were done using SPSS version 26. The degree of association between exogenous and endogenous variables was assessed and validated using structural equation modeling by AMOS version 21.Result:A total of 1037 (96.2% response rate) diabetic patients participated in the study. Of them 407(39.25%), 95% CI: [36.4–42.2] were found to have an intention to use patient portal. Digital literacy (β =0.312, 95% CI: [0.154-0.465], p < 0.01) performance expectancy (β =0.303, 95% CI: [0.185-0.420], p < 0.01) effort expectancy (β = 0.25 95% CI: [0.131-0.392], p < 0.01) facilitating condition (β = 0.22 95% CI: [0.081-0.36], p < 0.01) and habit (β = 0.111 95% CI: [-0.009-0.227], p < 0.05) were significantly associated with intention to use patient portal. Effort expectancy and facilitating condition were positively moderated by gender.Conclusion:This study found that diabetes patient’s intention to use patient portal was low. Intervention in digital literacy, performance expectancy, effort expectancy, facilitating conditions and habit are needed to improve diabetic patient’s intention to use the patient portal.
Selecting out of virulent strains of C. perfringens by the preparation process of meat curries possess a public health threat: evidence from a retrospective study
Authors: Perera Manosha Lakmali , Perera Irosha , Ranasinghe Gerard 
Published: August 2023

Abstract
Heat resistant spore formation is favourable for selecting out of C. perfringens virulent strains by the preparation process of meat curries. When heat resistance coupled with enterotoxin production it possesses a public health threat. Hence, this study was conducted using a representative sub sample of stored 15 meat curry isolates of C.perfringens from a large sample based on a previous descriptive cross sectional study to compare heat-resistant spore (HRS) formation, C. perfringens enterotoxin (CPE) production, and combined HRS - CPE abilities of meat curry isolates of this bacterium obtained from eating houses within Colombo city. Representative subsample (12 chicken and 03 beef) of preserved C. perfringens cultures after complete confirming retrieved from the cold room. Stored culture was vortex mixed prior to inoculation. Subsequently, 20ml of freshly steamed thioglycollate medium was inoculated with 2ml of stored culture (preserved in cooked meat medium and they were incubated anaerobically at 370C for 24hrs. Sporulation endeavoured using 3 media: Duncan and Strong, Modified Duncan and Strong, and Sporulation Broth as described previously. Heat resistance was tested at 1000C for 1 h in distilled water and the production of enterotoxin was evaluated with a PET-RPLA kit as detailed previously. The differences in selected virulent factors by the variety of meat curry were statistically significant (p<0.05). Selecting out of virulent strains due to the process of curry preparation was evident from this retrospective study. This evidence needs to confirm with a large sample size refining methodologies.
Quantifying Benefit-Risk and Addressing Missing Data in Late-Stage Clinical Trials
Authors: Ganju Jitendra , Fritsch Arno , Mueller Katharina , Schloemer Patrick , Yu Ron 
Published: August 2023

Abstract
It is important in clinical trials to quantify the net benefit and reliably estimate the effect of treatment when some patients dropout prematurely. The current approach to assessing net benefit is subjective, and methods for handling missing data are inadequate or not easily accessible by those who are not subject matter experts. We investigate a method for quantifying benefit-risk and addressing missing data. For benefit-risk, an important set of efficacy and safety endpoints is hierarchically organized. Each pair of patients, one from each group, is compared on the first hierarchically arranged endpoint to determine which patient fared better (‘wins’). If the comparison results in a win, the remaining endpoints are not compared. If it is tied, the pair is compared on the next hierarchical endpoint, and so on. The results of all such pairwise comparisons are combined as a win ratio: #wins / #losses. It is proposed that the analyses are presented sequentially and cumulatively, so that the contribution of each component to benefit-risk can be assessed. For missing data, the reason for and timing of missingness are incorporated in the pairwise comparison. For example, if both patients in a pair dropout due to adverse events, the patient who dropped out later has won. Thus, the reason for and the timing of missing data are explicitly incorporated. The Phase 3 PATENT-1 study evaluating riociguat in pulmonary arterial hypertension and patient preferences of benefit vs risk for renal cell carcinoma is used for illustration of the methods. The methods proposed provide a more objective way to assess benefit-risk and address missing data.
PENINGKATKAN PARTISIPASI AKTIF PARA PELAYAN DI GKJ WIROBRAJAN MELALUI IBADAH PADANG PASKA PANDEMI COVID-19
Authors: Minarni Nani , Purwadi Joko , Haryono Nugroho Agus 
Published: August 2023

Tulisan ini merupakan hasil pelatihan kepemimpinan yang dilakukan oleh Tim Pengabdian Fakultas Teknologi Informasi UKDW pada tahapan pertama dari tiga tahapan pendampingan yang disepakati oleh pengurus GKJ Wirobrajan. Pelatihan ditujukan bagi pengurus gereja yang terdiri atas majelis, komisi dan pengurus wilayah, dengan tujuan untuk menguatkan motivasi sebagai para pelayan di GKJ Wirobrajan pasca pandemic Covid-19. Tujuan berikutnya yakni mengenalkan model kepemimpinan pengurus (steward leader) dalam gereja yang lebih menekankan pendekatan partisipatoris dalam pelayanan sehingga tidak bertumpu pada sosok pendeta jemaat semata. Harapan akhir dari pelatihan kepemimpinan yakni para pengurus dapat memaknai kembali pemahamannya tentang arti pelayanan, pendekatan partisipatif dalam pelayanan dan memiliki komitmen melayani yang didengar dan didukung oleh sesama pengurus gereja. Untuk mencapai tujuan tersebut, metode yang digunakan dalam pelatihan yakni interaktif – reflektif, dengan dibuatkan alur kegiatan berupa kombinasi antara ibadah, ceramah, dinamika kelompok, dan refleksi diri. Hasil akhir yang didapat setelah pelaksanaan tahap pertama, pengurus memiliki tiga hal penting sebagai dasar ke tahap berikutnya, yakni memaknai arti pelayanan secara otentik, kesadaran baru menjadi pelayan yang partisipatif dan refleksi personal yang menyentuh aspek spiritualitas seorang pelayan.

Kata Kunci: GKJ Wirobrajan; Pelayanan, Pemimpin Pengurus, Interaktif-Reflektif, Pendekatan Partisipatif
EDUKASI PEMBUATAN MASKER ORGANIK DARI BAHAN DASAR KOMBINASI BERAS DAN DAUN RICA DI DESA MAKU, KABUPATEN SIGI, SULAWESI TENGAH
Authors: Anggi Viany , Sujati Ni Wayan , Rakanita Yasinta 
Published: August 2023

Kegiatan pengabdian kepada masyarakat adalah satu kegiatan penting dalam Tridarma Perguruan Tinggi yang harus dilakukan untuk pemberdayaan masyarakat yang mandiri dan professional dari ketidakberdayaan masyarakat yang menjadi permasalahan secara global saat ini. Sekolah Tinggi Ilmu Farmasi Pelita Mas Palu memiliki Kebun Tanaman obat keluarga di daerah Desa Maku, Kecamatan Dolo, Kabupaten Sigi Sulawesi Tengah. Untuk itu sebagai sumber Tanaman Obat keluarga yang ada didaerah tersebut, sehingga dipilih untuk kegiatan Pengabdian kepada masyarakat. Kegiatan ini bertujuan untuk memberikan pengetahuan kepada masyarakat desa Maku tentang pembuatan masker organik dari bahan dasar kombinasi bahan dasar beras dan daun rica yang berfungsi sebagai antioksidan dalam meregenerasi sel-sel kulit. Target luaran yang diharapkan dari kegiatan ini yaitu menumbuhkan jiwa kewirausahaan adanya produk-produk yang dihasilkan, memberikan penyuluhan, membuat kebun tanaman obat keluarga, memberikan pemeriksaan kesehatan gratis untuk masyarakat desa Maku dan pemberian sembako gratis. Metode yang digunakan dalam kegiatan ini yaitu pembentukkan kelompok perdusun sesuai dengan urutan dusun di desa Maku, penyampaian materi yang akan disampaikan, dimana 30% peserta belum memahami tentang pembuatan masker organik, penyampaian materi selama 1 jam dilanjutkan dengan melakukan penanaman TOGA, pemeriksaan kesehatan gratis dan pemberian sembako gratis. Hasil kegiatan menunjukkan bahwa masyarakat yang ada di desa Maku mampu memahami tentang materi edukasi pembuatan masker organic dengan tingkat pemahaman sebesar 70%.
Partons as unique ground states of quantum Hall parent Hamiltonians: The case of Fibonacci anyons
Authors: Tanhayi Ahari Mostafa , Bandyopadhyay Sumanta , Nussinov Zohar , Seidel Alexander , Ortiz Gerardo 
Published: August 2023

We present microscopic, multiple Landau level, (frustration-free and positive semi-definite) parent Hamiltonians whose ground states, realizing different quantum Hall fluids, are parton-like and whose excitations display either Abelian or non-Abelian braiding statistics. We prove ground state energy monotonicity theorems for systems with different particle numbers in multiple Landau levels, demonstrate S-duality in the case of toroidal geometry, and establish complete sets of zero modes of special Hamiltonians stabilizing parton-like states, specifically at filling factor \nu=2/3ν=2/3. The emergent Entangled Pauli Principle (EPP), introduced in [Phys. Rev. B 98, 161118(R) (2018)] and which defines the “DNA” of the quantum Hall fluid, is behind the exact determination of the topological characteristics of the fluid, including charge and braiding statistics of excitations, and effective edge theory descriptions. When the closed-shell condition is satisfied, the densest (i.e., the highest density and lowest total angular momentum) zero-energy mode is a unique parton state. We conjecture that parton-like states generally span the subspace of many-body wave functions with the two-body MM-clustering property within any given number of Landau levels, that is, wave functions with MMth-order coincidence plane zeroes and both holomorphic and anti-holomorphic dependence on variables. General arguments are supplemented by rigorous considerations for the M=3M=3 case of fermions in four Landau levels. For this case, we establish that the zero mode counting can be done by enumerating certain patterns consistent with an underlying EPP. We apply the coherent state approach of [Phys. Rev. X 1, 021015 (2011)] to show that the elementary (localized) bulk excitations are Fibonacci anyons. This demonstrates that the DNA associated with fractional quantum Hall states encodes all universal properties. Specifically, for parton-like states, we establish a link with tensor network structures of finite bond dimension that emerge via root level entanglement.
Da Inserção Profissional à Perseverança Docente
Authors: Damasco Denise Gisele de Britto , Passos Laurizete Ferragut 
Published: August 2023

O artigo discute a trajetória profissional de docentes do Distrito Federal em um estudo longitudinal visando compreender suas aprendizagens docentes e profissionais. Há dois contextos de pesquisa, sendo o primeiro realizado entre 2010 e 2014 (pesquisa doutoral) e o segundo contexto cerca de dez anos depois (pesquisa de pós-doutoramento). A inserção profissional e a perseverança na carreira docente constituem o quadro teórico, destacando a contribuição da pesquisadora Joséphine Mukamurera e de pesquisadores do contexto francófono sobre essa temática. Buscamos as aproximações metodológicas entre o Método Documentário e o método Análise de Prosa na compreensão de trajetórias de profissionais do DF, tendo em vista que os dois métodos de análises foram utilizados, para compreender as trajetórias dos docentes. As análises e interpretações evidenciam que não há um modelo de inserção profissional, que a perseverança não depende apenas de formação continuada que se realiza ao longo da carreira, do sentimento de êxito no início da carreira, havendo inúmeros fatores que complexifica a profissão docente, dentre os quais as condições de trabalho e outras condições subjetivas inerentes a ele.
Targeted Glioblastoma Treatment via Synthesis and Functionalization of Gold Nanoparticles With De Novo–Engineered Transferrin-Like Peptides: Protocol for a Novel Method
Authors: Müller Fiedler Augusto , Medeiros Michelle , Fiedler Haidi Dalinda 
Published: August 2023

Background
Glioblastoma multiforme (GBM) is an aggressive brain tumor with limited treatment options due to the blood-brain barrier’s (BBB’s) impedance and inherent resistance to chemotherapy. Gold nanoparticles (AuNPs) functionalized with transferrin-like peptides show promise in overcoming these challenges, enhancing drug delivery to the brain, and reducing chemotherapy resistance.

Objective
The primary goal of this study is to establish a detailed protocol for synthesizing and stabilizing AuNPs, functionalizing them with de novo–engineered transferrin-like peptides, and conjugating them with the chemotherapeutic agent temozolomide. This strategy aims to improve drug delivery across the BBB and circumvent chemotherapy resistance. The secondary objective includes an assessment of the safety and potential for in vivo use of the synthesized nanoparticle complex.

Methods
The proposal involves multiple steps with rigorous quality control of AuNP synthesis, stabilization with surfactants, and polyethylene glycol coating. The engineered transferrin-like peptides will be synthesized and attached to the AuNPs’ surface, followed by the attachment of temozolomide and O6-methylguanine-DNA methyltransferase inhibitors. The resulting complex will undergo in vitro testing to assess BBB penetration, efficacy against GBM cells, and potential toxicity.

Results
Initial preliminary experiments and simulations suggest successful synthesis and stabilization of AuNPs and effective attachment of transferrin-like peptides. We propose peptide attachment verification using Fourier transform infrared spectroscopy and surface plasmon resonance. Additionally, we will conduct pH stability tests to ensure our functionalized AuNPs retain their properties in acidic brain tumor microenvironments.

Conclusions
The proposed functionalization of AuNPs with de novo–engineered transferrin-like peptides represents a novel approach to GBM treatment. Our strategy opens new avenues for drug delivery across the BBB and chemotherapy resistance reduction. While we primarily focus on in vitro studies and computational modeling at this stage, successful completion will lead to further development, including in vivo studies and nanoparticle design optimization. This proposal anticipates inspiring future research and funding in neuro-oncology, presenting a potentially innovative and effective treatment option for GBM.

International Registered Report Identifier (IRRID)
RR1-10.2196/49417
Detection of <i>AtRKD4</i> Gene and Induction of Somatic Embryo in Transformant of <i>Phalaenopsis amabilis</i> Carrying 35S::GR::<i>AtRKD4</i>
Authors: Sundari Dika , Perdana Naufal Ghozi Aditya , Mose Windi , Gutierrez -Marcos Jose , Semiarti Endang 
Published: August 2023

Phalaenopsis amabilis (L.) Blume is a native Indonesian orchid that plays an important role in the breeding of orchid’s hybrid worldwide. The high consumer demand causes a decline in the population of orchids for commercial trade. Plant propagation through induction of somatic embryogenesis will be very beneficial, because plants can be obtained in large numbers and uniform. AtRKD4 gene is an important gene in the model plant Arabidopsis thaliana which functions very early in development stage to initiate embryo formation. The AtRKD4 gene has been inserted into the P. amabilis orchid and several transformants have been obtained. This study aims to determine the stability integration of the AtRKD4 gene in the transformant P. amabilis genome and induce somatic embryo formation on transformant orchids. Plantation of leaf explants from P. amabilis transformants on hormone-free New Phalaenopsis basic medium induced somatic embryo formation by 20%. Anatomical analysis showed that there is no difference stage between anatomy of somatic embryo development pattern in P. amabilis transformant and somatic embryo development pattern of monocot plants in general, PCR analysis using AtRKD4 specific primers showed that the embryos still contained 198 bp fragments of the AtRKD4 gene. In conclusion, the AtRKD4 gene is stably integrated in the P. amabilis orchid genome and can continuously induce the formation of somatic embryo from somatic cells of orchid transformants.
DNA Authentication of Indonesian Leaffish <i>Pristolepis grooti</i> from Kelekar River and Ogan River in South Sumatra Based on <i>Cytochrome C Oxidase Subunit I (COI)</i> Gene
Authors: Syaifudin Mochamad 
Published: August 2023

Indonesian leaffish Pristolepis grooti, an endemic species, are distributed in the region of Sumatra, Riau, Bangka Belitung and Kalimantan. However, there has been a decline in the population recently. This research purposed to investigate the mitochondrial DNA cytochrome c oxidase subunit I (COI) gene, the genetic distance, the genetic tree of the leaffish and characterize the chemical physics of water of its habitat in the Kelekar River Muara Enim Regency and the Ogan River, Ogan Ilir Regency. The method used in species authentication was DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of COI gene. The size of the COI mtDNA gene fragment was 704 bp (PM 1, PM 4, PP 2 and PP 4) and 723 bp (PM 2, PM 3, PP 1 and PP 3). A cryptic diversity of the species P. grooti is found based on the genetic distance value of 4.5-6%, both in the Kelakar and Ogan Rivers. The phylogenetic tree of the leaffish of this study formed 2 separate sub-clusters with a bootstrap value of 50%. The properties of water qualities in the two rivers included temperatures 28.3-31.8°C, pH 5.6-8.3, dissolved oxygen 4.82-10.89 mg L-1, alkalinity 10-28 mg L-1 CaCO3, water transparency 16-45 cm, ammonia 0.47-0.70 mg L-1, water current 0.17-0.30 m s-1 and TDS 7-44 mg L-1.
Rancang Bangun Sistem Monitoring Pada Line Produksi Menggunakan Weintek CMT FHDX
Authors: Fariz Muhammad Iqbal , Basuki Agung Yoke 
Published: August 2023

Abstrak— Sistem monitoring adahlah sistem yang digunakan untuk memantau dan melaporkan hasil dari suatu sistem mesin produksi, apabila sistem monitoring dilakukan dengan cara manual seperti mencatat hasil dari produksi yang kemudian akan dilaporkan ke atasan atau penanggung jawab di produksi yang kemudian baru akan diteruskan kepada pihak kantor yang akan merubahnya ke dalam komputer, proses ini tentu nya akan memakan waktu yang lama, oleh karena itu digunakannya sistem monitoring otomatis untuk dapat memudahkan monitoring dan melaporkan hasil dari suatu sistem mesin produksi. Untuk dapat membuat sistem tersebut digunakan PLC dan HMI, PLC digunakan untuk menarik informasi dari suatu mesin melalui sensor atau tombol perintah untuk membaca atau menarik informasi kemudian PLC akan mengirim hasil data kepada HMI untuk dapat disimpan dan ditampilkan melalui laptop dan handphone android. Setelah perancangan sistem monitoring didapatkan hasil pengujian penelitian sistem monitoring dapat memonitoring hasil dari mesin produksi melalui laptop dan handphone android, waktu rata rata yang dibutuhkan untuk dapat mendapat data hasil produksi hingga termonitoring ke pengguna adalah 11,6 milisecond dan 13,8 milisecond yang di ukur menggunakan stopwatch milisecond, akurasi dari hasil monitoring pun akurat 100% dengan pengambilan sampel 10 data tanpa terdapat kesalahan satu pun.
Impact of New Energy Vehicles on Average Fuel Consumption of BYD Auto’s Passenger Vehicles
Authors: Li Jia C. 
Published: August 2023

The impact of new energy vehicles (NEV) on average fuel consumption of automakers’ passenger vehicles in China，is a question that has not been clearly addressed. In this paper, average accounting fuel consumptions and average real fuel consumptions of BYD Auto (hereinafter referred to as "BYD")’s passenger vehicles, as well as average fuel consumptions of BYD’s NEV and average fuel consumptions of BYD’s conventional energy vehicles (CEV) from 2016 to 2021, were calculated. Then the impact of production multiplier of NEV and low average fuel consumption of NEV were distinguished to reveal the path and extent of NEV’s impact on average fuel consumption of BYD's passenger vehicles.
The results showed that, Production multiplier of NEV and low average fuel consumption of NEV are key factors by which NEV promoted the decline of average fuel consumption of BYD’s passenger vehicles. Production multiplier of NEV caused the difference between average accounting fuel consumption and average real fuel consumption of BYD’s passenger vehicles, Low average fuel consumption of NEV caused the difference between average real fuel consumption of BYD’s passenger vehicles and average fuel consumption of BYD’s CEV, and the total impact of NEV on the average accounting fuel consumption of BYD’s passenger vehicles was equal to the sum of the above two differences, which is equal to average accounting fuel consumption of BYD’s passenger vehicles minus average fuel consumption of BYD’s CEV.