bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extended validation of the Relational Depth Frequency Scale: Retest reliability, divergent validity, criterion validity with psychotherapy satisfaction, and measurement invariance in UK‐ and US‐stratified samples
Authors: Di Malta Gina , She Zhuang , Raymond‐Barker Brett , Cooper Mick 
Published: April 2023

AbstractBackgroundThe Relational Depth Frequency Scale (RDFS) assesses moments of profound connection in psychotherapy, associated with therapeutic benefit. To date, the RDFS has not been tested for its retest reliability, divergent and criterion validity, and measurement invariance, nor has it been tested in stratified samples of psychotherapy patients.MethodsTwo stratified online samples of United Kingdom (n = 514) and United States (n = 402) psychotherapy patients filled out the RDFS, the Brief Social Desirability Scale (BSDS); and the Satisfaction with Therapy and Therapist Scale‐revised (STTS‐R). Two subsamples of patients (United Kingdom: n = 50 and United States: n = 203) filled out the RDFS again after 1 month.ResultsReliability for the six‐item RDFS were excellent in United Kingdom and United States samples (Cronbach's α = 0.91 and 0.92; retest r = 0.73 and r = 0.76). Divergent (r = 0.10 and r = 0.12) and criterion validity (r = 0.69; and r = 0.70) were good. Full scalar invariance was established across countries, genders, and time.ConclusionThis contributes important evidence to the validity of the RDFS. Future research should assess predictive validity against psychotherapy outcomes and replicate these analyses in diverse samples.
Ginkgolide B facilitates muscle regeneration via rejuvenating osteocalcin‐mediated bone‐to‐muscle modulation in aged mice
Authors: Wang Belle Yu‐Hsuan , Chen Yi‐Fan , Hsiao Allen Wei‐Ting , Chen Wan‐Jing , Lee Chien‐Wei , Lee Oscar Kuang‐Sheng 
Published: April 2023

AbstractBackgroundThe progressive deterioration of tissue–tissue crosstalk with aging causes a striking impairment of tissue homeostasis and functionality, particularly in the musculoskeletal system. Rejuvenation of the systemic and local milieu via interventions such as heterochronic parabiosis and exercise has been reported to improve musculoskeletal homeostasis in aged organisms. We have shown that Ginkgolide B (GB), a small molecule from Ginkgo biloba, improves bone homeostasis in aged mice by restoring local and systemic communication, implying a potential for maintaining skeletal muscle homeostasis and enhancing regeneration. In this study, we investigated the therapeutic efficacy of GB on skeletal muscle regeneration in aged mice.MethodsMuscle injury models were established by barium chloride induction into the hind limb of 20‐month‐old mice (aged mice) and into C2C12‐derived myotubes. Therapeutic efficacy of daily administrated GB (12 mg/kg body weight) and osteocalcin (50 μg/kg body weight) on muscle regeneration was assessed by histochemical staining, gene expression, flow cytometry, ex vivo muscle function test and rotarod test. RNA sequencing was used to explore the mechanism of GB on muscle regeneration, with subsequent in vitro and in vivo experiments validating these findings.ResultsGB administration in aged mice improved muscle regeneration (muscle mass, P = 0.0374; myofiber number/field, P = 0.0001; centre nucleus, embryonic myosin heavy chain‐positive myofiber area, P = 0.0144), facilitated the recovery of muscle contractile properties (tetanic force, P = 0.0002; twitch force, P = 0.0005) and exercise performance (rotarod performance, P = 0.002), and reduced muscular fibrosis (collagen deposition, P < 0.0001) and inflammation (macrophage infiltration, P = 0.03). GB reversed the aging‐related decrease in the expression of osteocalcin (P < 0.0001), an osteoblast‐specific hormone, to promote muscle regeneration. Exogenous osteocalcin supplementation was sufficient to improve muscle regeneration (muscle mass, P = 0.0029; myofiber number/field, P < 0.0001), functional recovery (tetanic force, P = 0.0059; twitch force, P = 0.07; rotarod performance, P < 0.0001) and fibrosis (collagen deposition, P = 0.0316) in aged mice, without an increased risk of heterotopic ossification.ConclusionsGB treatment restored the bone‐to‐muscle endocrine axis to reverse aging‐related declines in muscle regeneration and thus represents an innovative and practicable approach to managing muscle injuries. Our results revealed the critical and novel role of osteocalcin–GPRC6A‐mediated bone‐to‐muscle communication in muscle regeneration, which provides a promising therapeutic avenue in functional muscle regeneration.
Histopathological correlations and fat replacement imaging patterns in recessive limb‐girdle muscular dystrophy type 12
Authors: De Wel Bram , Huysmans Lotte , Depuydt Christophe E. , Goosens Veerle , Peeters Ronald , Santos Filipa P. , Thal Dietmar R. , Dupont Patrick , Maes Frederik , Claeys Kristl G. 
Published: April 2023

AbstractBackgroundDespite the widespread use of proton density fat fraction (PDFF) measurements with magnetic resonance imaging (MRI) to track disease progression in muscle disorders, it is still unclear how these findings relate to histopathological changes in muscle biopsies of patients with limb‐girdle muscular dystrophy autosomal recessive type 12 (LGMDR12). Furthermore, although it is known that LGMDR12 leads to a selective muscle involvement distinct from other muscular dystrophies, the spatial distribution of fat replacement within these muscles is unknown.MethodsWe included 27 adult patients with LGMDR12 and 27 age‐matched and sex‐matched healthy controls and acquired 6‐point Dixon images of the thighs and T1 and short tau inversion recovery (STIR) MR images of the whole body. In 16 patients and 15 controls, we performed three muscle biopsies, one in the semimembranosus, vastus lateralis, and rectus femoris muscles, which are severely, intermediately, and mildly affected in LGMDR12, respectively. We correlated the PDFF to the fat percentage measured on biopsies of the corresponding muscles, as well as to the Rochester histopathology grading scale.ResultsIn patients, we demonstrated a strong correlation of PDFF on MRI and muscle biopsy fat percentage for the semimembranosus (r = 0.85, P < 0.001) and vastus lateralis (r = 0.68, P = 0.005). We found similar results for the correlation between PDFF and the Rochester histopathology grading scale. Out of the five patients with inflammatory changes on muscle biopsy, three showed STIR hyperintensities in the corresponding muscle on MRI. By modelling the PDFF on MRI for 18 thigh muscles from origin to insertion, we observed a significantly inhomogeneous proximo‐distal distribution of fat replacement in all thigh muscles of patients with LGMDR12 (P < 0.001), and different patterns of fat replacement within each of the muscles.ConclusionsWe showed a strong correlation of fat fraction on MRI and fat percentage on muscle biopsy for diseased muscles and validated the use of Dixon fat fraction imaging as an outcome measure in LGMDR12. The inhomogeneous fat replacement within thigh muscles on imaging underlines the risk of analysing only samples of muscles instead of the entire muscles, which has important implications for clinical trials.
Stability of labile xanthones and dihydrochalcones in a ready‐to‐drink honeybush beverage during storage
Authors: Human Chantelle , de Beer Dalene , Tredoux Andreas , de Villiers André , Joubert Elizabeth 
Published: April 2023

AbstractBACKGROUNDThe shelf‐life of a functional herbal tea‐based beverage is important not only for consumer acceptability, but also for the retention of bioactive compounds. The present study aimed to clarify the role of common iced tea beverage ingredients (citric and ascorbic acids) on the shelf‐life stability of an herbal tea‐based beverage. A hot water extract of green Cyclopia subternata, also used as honeybush tea, was selected as the main ingredient because it provides different types of phenolic compounds associated with bioactive properties (i.e. xanthones, benzophenones, flavanones, flavones and dihydrochalcones).RESULTSThe model solutions were stored for 180 and 90 days at 25 and 40 °C, respectively. Changes in their volatile profiles and color were also quantified as they contribute to product quality. 3′,5′‐Di‐β‐d‐glucopyranosyl‐3‐hydroxyphloretin (HPDG; dihydrochalcone) and, to a lesser extent, mangiferin (xanthone), were the most labile compounds. Both compounds were thus identified as critical quality indicators to determine shelf‐life. The stability‐enhancing activity of the acids depended on the compound; ascorbic acid and citric acid enhanced the stability of HPDG and mangiferin, respectively. However, when considering all the major phenolic compounds, the base solution without acids was the most stable. This was also observed for the color and major volatile aroma‐active compounds [α‐terpineol, (E)‐β‐damascenone, 1‐p‐menthen‐9‐al and trans‐ocimenol].CONCLUSIONThe addition of acids, added for stability and taste in ready‐to‐drink iced tea beverages, could thus have unwanted consequences in that they could accelerate compositional changes and shorten the shelf‐life of polyphenol‐rich herbal tea beverages. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis
Authors: He Sihan , Li Jiong , Wang Yunjia , Xiang Gang , Yang Guanteng , Xiao Lige , Tang Mingxing , Zhang Hongqi 
Published: April 2023

AbstractBackgroundAdolescent idiopathic scoliosis (AIS) is a scoliotic deformity of unknown etiology that occurs during adolescent development. Abnormal bone metabolism is closely related to AIS, but the cause is uncertain. Recent studies have shown that heat shock protein 27 (HSP27) and its phosphorylation (pHSP27) play important roles in bone metabolism. However, whether HSP27 and pHSP27 are involved in abnormal bone metabolism in AIS is unclear.MethodsOsteoblasts (OBs) and bone marrow stem cells (BMSCs) were extracted from the facet joints and bone marrow of AIS patients and controls who underwent posterior spinal fusion surgery. The expression levels of HSP27 and pHSP27, as well as the expression levels of bone formation markers in OBs from AIS patients and controls, were examined by quantitative real‐time PCR (qRT–PCR) and Western blotting. The mineralization ability of OBs from AIS patients and controls was analyzed by alizarin red staining after osteogenic differentiation. Heat shock and thiolutin were used to increase the levels of pHSP27 in OBs, and the levels of bone formation markers were also investigated. In addition, the levels of pHSP27 and the bone formation ability of BMSCs from AIS patients and controls were investigated after heat shock treatment.ResultsLower pHSP27 levels and impaired osteogenic differentiation abilities were observed in the OBs of AIS patients than in those of controls. Thiolutin increased HSP27 phosphorylation and increased the mRNA levels of SPP1 and ALPL in OBs from AIS patients. Heat shock treatment increased SPP1 and HSP27 mRNA expression, pHSP27 levels, OCN expression, and mineralization ability of both OBs and BMSCs from AIS patients.ConclusionHeat shock treatment and thiolutin can increase the levels of pHSP27 and further promote the bone formation of OBs and BMSCs from AIS patients. Therefore, decreased pHSP27 levels may be associated with abnormal bone metabolism in AIS patients.
Pattern diagnosis of onycholysis
Authors: Navarro Lourdes 
Published: April 2023

AbstractOnycholysis is defined as the detachment of the nail plate from the underlying and/or lateral supporting structures. Trauma, inflammatory conditions, infectious agents, tumours and toxic substances are the most commonly reported causes of onycholysis. We review the main causes of onycholysis in clinical practice and provide a diagnostic approach based on morphologic patterns of the proximal border of onycholysis, as defined by clinical and dermoscopic examination. Four morphologic patterns of onycholysis, namely transversal, lateral, V‐shaped and circular are presented with their most common causes and triggers.
The effects of two different biochars on the characteristics of saline acid sulfate soil
Authors: Nguyen Tung Xuan Tan , Nguyen Binh Thanh 
Published: April 2023

AbstractBiochar can be a potential bio‐amendment for remediating saline acid sulfate soil and its effects may be dependent on its properties. The current study sought to assess the effects of two different biochars on the properties and quality of saline acid sulfate soil. The experiment was implemented by mixing soil with biochars made from longan branches (LBs) and coconut coir biochar (CB) at five rates of 0%, 0.7%, 1.5%, 3%, and 6% (w/w). After 100 days of incubation in a greenhouse, soil samples from experimental pots were taken and analyzed for 11 parameters. The results showed that biochar significantly enhanced pH while declining the concentration of SO42− and exchangeable Al and Fe. Mechanisms responsible for these effects could be due to the high alkaline properties of the added biochar. Coconut biochar raised the concentration of exchangeable Na, K, and Mg more strongly than LB. Consequently, coconut biochar greatly increased the soil electrical conductivity (EC) value, while longan biochar exhibited no significant effect. The soil quality index of the 6%‐LB‐added soil increased by 64% and that of the 6%‐CB‐added soil increased by 45%, compared with the zero‐biochar‐added soil. Lower soil quality induced by CB compared with LB may be attributed to CB's initial properties, which had higher EC, Cl−, and Na levels than LB. The findings suggested that biochar addition can improve overall quality but alter individual properties of saline acid sulfate soil in different directions, depending on the properties of the added biochar, which requires more studies to clarify its ameliorative effects.
Risk factors for developing subglottic and tracheal stenosis from the medical intensive care unit
Authors: Pappal Robin B. , Burruss Clayton Prakash , Witt Michael A. , Harryman Christopher , Ali Syed Z. , Bush Matthew L. , Fritz Mark A. 
Published: April 2023

AbstractObjectiveEndotracheal intubation is a common procedure in the medical intensive care unit (MICU), but it carries risk of complications including, but not limited to, subglottic stenosis (SGS) and tracheal stenosis (TS). Current literature suggests identifiable risk factors for the development of airway complications. This study is a comprehensive evaluation of potential risk factors in patients who developed SGS and TS following endotracheal intubation in our MICU.MethodsPatients intubated in our MICU were identified from 2013 to 2019. Diagnoses of SGS or TS within 1 year of MICU admission were identified. Data extracted included age, sex, body measurements, comorbidities, bronchoscopies, endotracheal tube size, tracheostomy, social history, and medications. Patients with prior diagnosis of airway complication, tracheostomy, or head and neck cancer were excluded. Univariate and multivariate logistic regressions were performed.ResultsA total of 136 patients with TS or SGS were identified out of a sample of 6603 patients intubated in the MICU. Cases were matched to controls who did not develop airway stenosis based on identical Charlson Comorbidity Index scores. Eighty six controls were identified with a complete record of endotracheal/tracheostomy tube size, airway procedures, sociodemographic data, and medical diagnosis. Regression analysis showed that SGS or TS were associated with tracheostomy, bronchoscopy, chronic obstructive pulmonary disease, current tobacco use, gastroesophageal reflux disease, systemic lupus erythematosus, pneumonia, bronchitis, and numerous medication classes.ConclusionVarious conditions, procedures, and medications are associated with an increased risk of developing SGS or TS.Level of evidence4.
Preparation and intraocular evaluation of cetirizine hydrochloride ophthalmic liposomes and a liposome in situ gel
Authors: Zhang Jing , Wang Lan , Li Jianghong , Guo Yuhong , Chen Jinxin , Li Xudong , Cheng Man , Feng Bo , Zhang Ying 
Published: April 2023

AbstractCetirizine hydrochloride (CTZ), an antiallergic drug, is a new‐generation H1 receptor antagonist and a second‐generation H1 antihistamine. We aimed to prepare cetirizine hydrochloride liposomes, based on which cetirizine hydrochloride liposomal (CTZL) in situ gel (ISG) was prepared, to improve the retention time in the eye. CTZL were prepared by the ethanol injection method combined with the ammonium sulfate gradient method. A CTZ liposomal temperature‐sensitive gel was prepared using the cold dissolution method. Large‐eared white rabbits were used in retention and irritation experiments. The liposomes were small single‐chambered liposomes, spherical or sphere‐like, with a vesicle size of 187.03 ± 6.20 nm, an encapsulation efficiency of 70.39 ± 1.13%, and a drug loading capacity of 4.63 ± 0.06%. The gelling temperatures before and after dilution by simulated tear fluid were 26.1 ± 0.2°C and 34.2 ± 0.2°C, the vesicle size was 184.94 ± 7.28 nm, and the liposomes were spherical or sphere‐like in the gel matrix. The in vitro dissolution and release experiments indicate that the gel was released upon dissolution and exhibited a zero‐level release pattern. Preparation into liposomes and liposomal gels prolonged the ocular retention time of the formulation without ocular irritation.
Barriers in cancer trajectories of patients with pre‐existing severe mental disorders—A systematic review
Authors: Bentson Terese Myhre , Fløe Louise E. , Bruun Josefine M. , Eriksen Jesper Grau , Johnsen Søren Paaske , Videbech Poul , Brogaard Trine , Andreassen Pernille , Mygind Anna , Bøndergaard Kirstine B. , Neergaard Mette Asbjoern 
Published: April 2023

AbstractBackgroundPatients with pre‐existing severe mental disorders are significantly less likely to receive guideline‐recommended cancer treatment and seems to have a significantly lower rate of cancer survival compared to patients with cancer without mental disorders.AimTo perform a systematic review on barriers at patient‐, provider‐ and system‐levels in cancer trajectories of patients with pre‐existing severe mental disorders.MethodA systematic review was performed following the PRISMA guidelines (PROSPERO ID: CRD42022316020).ResultsNine eligible studies were identified. Barriers at patient‐level included lack of self‐care and ability to recognize physical symptoms and signs. Provider‐level barriers included stigma from health care professionals on mental disorders, whereas system‐level barriers included fragmented health care and consequences of this.ConclusionThis systematic review found that barriers at patient‐, provider‐ and system‐levels exist in cancer trajectories for patients with severe mental disorders, causing disparities in cancer care. Further research is needed to improve cancer trajectories for patients with severe mental disorder.
Computational design and molecular modeling of morphine derivatives for preferential binding in inflamed tissue
Authors: Augenstein Makena , Alexander Nayiri , Gartner Matthew 
Published: April 2023

AbstractThe opioid epidemic has impacted over 10 million Americans in 2019. Opioids, like morphine, bind non‐selectively in both peripheral tissue, leading to effective pain relief, and central tissue, resulting in dangerous side effects and addiction. The inflamed conditions of injured tissues have a lower pH (pH = 6–6.5) environment than healthy tissue (pH = 7.4). We aim to design a morphine derivative that binds selectively within inflamed tissue using molecular extension and dissection techniques. Morphine binds to the μ‐opioid receptor (MOR) when the biochemically active amine group is protonated. Fluorination of a β‐carbon from the tertiary amine group led to a reduced pKa of the derivative through induction. Through a decrease in the pKa, protonation is still statistically favored in lower pH environments of inflamed tissue but primarily deprotonated in healthy tissue. The cyclohexenol and N‐methyl‐piperidine rings of morphine are removed to increase conformational flexibility when binding while still maintaining the interactions required for analgesia. Electronic structure calculations were performed with Gaussian16 using the Keck Computational Research Cluster at Chapman University to determine the pKa. The theoretical pKa values are determined at the M06‐2X(SMD)/aug‐cc‐pVDZ level of theory to calculate the ΔG°aq values for the amine deprotonation reactions. Fluoromorphine β‐C2 was designed computationally and modeled within the MOR using Maestro: Schrödinger. This derivative exhibits a pKa reduction and enhanced ligand‐protein interactions within the MOR. β‐fluorination decreased the overall pKa values of the morphine derivatives (pKa: 6.1–7.83) relative to morphine, reducing binding within healthy, central tissue.
A method for time‐varying reliability calculation of RC bridges considering random deterioration process
Authors: Yang Yong , Li Haibin , Huang Hong‐Zhong 
Published: April 2023

AbstractThis paper proposes a new method based on dual neural networks to improve the efficiency of time‐varying reliability calculation of bridge structures. Based on the basic theory of structural time‐varying reliability analysis, this method uses the dyadic neural network integration method to convert the time‐varying reliability calculation of bridges from the traditional Monte Carlo simulation to the direct integration operation, which improves operation efficiency and retains high working accuracy. In addition, considering the randomness of the decay process of different bridge resistances, the variation law of time‐varying reliability of bridges in the process of uncertain resistance reduction is studied, and an explicit formula for time‐varying reliability of bridges is given and solved by using the proposed dual neural network method. Finally, the effectiveness of the proposed method is verified by an engineering arithmetic example. The result indicates that the bridge has a greater probability of failure under random conditions.
Selective Ion‐pair Potentiometric Sensor for the Determination of Ondansetron Hydrochloride in Different Matrixes
Authors: Dyab Abdalqader , Darwish Maher , Al Rahal Fatema , Moustapha Aoula 
Published: April 2023

AbstractA facile, novel, and, sensitive potentiometric sensor has been developed to quantitatively determine ondansetron hydrochloride in bulk and pharmaceutical dosage forms. The sensor comprised an ion‐pair between ondansetron hydrochloride and ammonium reineckate reagent, as an electrochemically active substance, on a polyvinyl chloride (PVC) matrix. Synthesis optimization was carried out by firstly altering the proportion of the ion‐pair, PVC, and the plasticizer and subsequently, many plasticizer types have been investigated to realize the best performance. The optimum electrode consisted of 48.5 % polyvinyl chloride, 48.5 % dibutyl phthalate, and 3 % ionic pair complex and has shown a respectful Nernstian relationship within a linear range of (5.06×10−6–2592.92×10−6 M) and a slope of 57.277 mV×decade−1 in response to the drug. The electrode worked perfectly in a pH range of (1.9–6.1) where the analyte kept dissolved. Interestingly, the electrode demonstrated a fast response time of 30 sec, a lower limit of detection of 4.83×10−6 M, and an electrode lifetime of up to 30 days. The prepared electrode was applied successfully to determine the drug in its pharmaceutical dosage forms and the method validity was established. Hence, the electrode might be applied easily for the routine analysis of ondansetron hydrochloride in both scientific labs and drug industry.