bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human epidermal growth factor receptor 2 inhibits activating transcription factor 7 to promote breast cancer cell migration by activating histone lysine demethylase 1
Authors: Lin Juli , Mao Hehui , Ji Zhuannan , Lin Weijie , Wang Tao 
Published: March 2023

Background:
Receptor tyrosine-protein kinase erbB-2 (human epidermal growth factor receptor 2 [HER2])-based therapies can improve the prognosis of HER2-positive breast cancer (BRCA) patients; however, HER2-positive patients with distal metastasis do not gain significant clinical benefit from molecular targeted therapy.

Materials and Methods:
A database analysis, immunohistochemistry, and quantitative real-time polymerase chain reaction were used to evaluate the expression of activating transcription factor 7 (ATF7) and its clinical value. A transwell chamber assay was used to assess migration and cell signaling was assessed by immunoblotting.

Results:

ATF7 was expressed at a low level in HER2-enriched BRCA specimens compared with normal or HER2-negative specimens, which was corroborated in HER2-positive tissue chips and cultured cells. ATF7 gradually decreased with increased tumor stage and low ATF7 was associated with poor prognosis in HER2-positive BRCA patients. ATF7-upregulation inhibited, whereas ATF7-knockdown promoted migration, activity of matrix metalloproteinase 9 (MMP9), MMP2, and uridylyl phosphate adenosine and plasminogen activator inhibitor-1 (PAI-1) expression in HER2-positive cells. HER2 overexpression markedly reduced ATF7 expression in MCF-10A mammary epithelial cells, along with decreased E-cadherin, and increased N-cadherin and migration, which were abrogated by exogenous ATF7 transfection. Mechanistically, HER2 upregulation mediated the decline of ATF7 and activated histone lysine demethylase 1 (LSD1), followed by elevation of histone H3K9 dimethylation (H3K9me2) and H3K4me2. However, the enhanced effects on LSD1 and H3K9me2, excluding H3K4me2, were abrogated by exogenous ATF7. ATF7 was negatively associated with KDM1A (encoding LSD1 protein) expression.

Conclusions:

ATF7 may be a useful diagnostic and prognostic marker for metastatic HER2-positive BRCA. The ATF7/LSD1/H3K9me2 axis may be responsible for metastasis in HER2-positive cells.
Comparing endoscopic thyroidectomy using the breast approach and conventional open thyroidectomy
Authors: Zhang Hanyuan , Shi Weichen , Zhang Jiqing , Xu Jia , Zhou Dongsheng , Liu Wei , Fu Rongzhan , Chen Hongqiang 
Published: March 2023

Aims:
Endoscopic thyroidectomy (ET) using the breast approach and conventional open thyroidectomy (OT) are effective approaches to treating thyroid tumors. This study evaluates the effectiveness of ET and OT regarding safety, cosmetic effects, and feasibility.

Subjects and Methods:
Four hundred and fifty-six patients who underwent thyroidectomy in our department from January 2019 to August 2020 were included in this study. Based on the intraoperative rapid pathology, all patients with papillary thyroid carcinoma underwent unilateral thyroid lobectomy and central neck lymph node dissection. Whereas all benign patients underwent unilateral thyroid lobectomy. Differences in various factors such as clinical characteristics, operation time, postoperative drainage volume, parathyroid hormone (PTH) levels, calcium (Ca) levels, total number of central lymph nodes resected, the number of metastatic central lymph nodes resected, hospital duration, hospitalization costs, and cosmetic effects were compared in each group.

Results:
Baseline characteristics among the four groups were similar, except for patient age and tumor size. Patients in the malignant ET group were younger than those in the malignant OT group with smaller tumors (P < 0.05). There were no significant differences between the OT and ET groups in postoperative Ca levels, PTH levels, the total number of lymph nodes resected, and the number of metastatic central lymph nodes resected.

Conclusions:
Compared with conventional OT, ET is a feasible, practical, and safe procedure with excellent cosmetic benefits.
The role of surgery on locoregional treatment of patients with breast cancer newly diagnosed with ipsilateral supraclavicular lymph node metastasis
Authors: Liu Bo-Wen , Chen Li-Xuan , Ma Ke , Chi Jiang-Rui , Yang Zheng-Jun , Yu Yue , Cao Xu-Chen 
Published: March 2023

Background:
Radiotherapy is a practical locoregional treatment approach for women with breast cancer who show ipsilateral supraclavicular lymph node metastasis (ISLNM) on diagnosis. However, there is controversy around the role of supraclavicular lymph node dissection. Therefore, we aimed to study the significance of supraclavicular surgery based on radiotherapy.

Patients and Methods:
We retrospectively reviewed the data of 142 patients with breast cancer who presented with isolated ISLNM and received radiotherapy between the years 2000 and 2016. We also defined the effect of surgery on locoregional treatment of these patients by analyzing the prognostic factors for recurrence-free survival (RFS), distant metastasis-free survival (DMFS), and overall survival (OS).

Results:
We observed that, of the 142 patients, 104 who received radiotherapy underwent supraclavicular lymph node dissection. Also, among the study group, the progesterone receptor (PR) status (P = 0.044) and the number of axillary lymph nodes (ALNs) involved (P = 0.002) were significant independent predictors of RFS. Also, tumor size (P = 0.007), PR (P < 0.001), and number of ALNs (P < 0.001) were independent predictors of DMFS and were statistically significant. Also, PR was an independent prognostic factor of OS (P = 0.033), whereas the supraclavicular surgery was not an independent prognostic factor for RFS, DMFS, and OS. Furthermore, our study focused on 92 patients with negative estrogen receptors (ERs). The result showed that supraclavicular surgery was statistically significant for RFS (P = 0.023); no significant differences in DMFS and OS were found between patients who received supraclavicular surgery and those who did not.

Conclusion:
Radiotherapy may be the primary locoregional treatment approach for patients with breast cancer who present with newly diagnosed ISLNM. Additionally, supraclavicular surgery may be more appropriate for patients with negative ER who received radiotherapy.
Effectiveness and safety of camrelizumab combined with chemotherapy in nonsquamous nonsmall cell lung cancer as the second-line therapy
Authors: Huang Wei , Zhang Qinyuan , Da Liangshan , Shen Yuanyuan , Xiong Fuxing , Zhang Congjun 
Published: March 2023

Background:
The role of camrelizumab combined with chemotherapy as the second-line therapy in nonsquamous nonsmall cell lung cancer (NSCLC) remains unverified. The retrospective study investigated efficacy and safety of camrelizumab combined with chemotherapy in the treatment of nonsquamous NSCLC as the second-line therapy.

Subjects and Methods:
Patients of nonsquamous NSCLC who were already discharged or died of the First Affiliated Hospital of Anhui Medical University between August 2019 and September 2020. According to the treatment method, the patients who received chemotherapy were denoted as the C group and those who received camrelizumab plus chemotherapy were denoted as the C&C group.

Statistical Analysis Used:
Patients responses were statistically analyzed. The Cox proportional hazards regression model was used in the assessment of the prognostic value of factors. Furthermore, adverse event evaluation was estimated.

Results:
Of the 60 patients with nonsquamous NSCLC included in the research, 29 patients received chemotherapy, and 31 patients received camrelizumab plus chemotherapy. The objective response rate was 13.79% and 32.26% for chemotherapy and camrelizumab plus chemotherapy groups, and the disease control rate was 72.41% and 80.65%. The median progression-free survival (mPFS) in camrelizumab plus chemotherapy group was obviously higher than that in the chemotherapy group (9.67 vs. 6.87 months, P = 0.01). The median overall survival of the camrelizumab plus chemotherapy was longer than the chemotherapy (10.89 vs. 7.95 months, P < 0.01). In the current treatment, radiotherapy and smoking were independent risk factors for the mPFS of patients with nonsquamous NSCLC. The occurrence of adverse events was similar between chemotherapy and camrelizumab plus chemotherapy groups.

Conclusions:
Camrelizumab combined with chemotherapy was an effective regimen with manageable toxicity in treating nonsquamous NSCLC as the second-line therapy.
Association of miR-155 and MIR155HG polymorphisms with cancer risk
Authors: Zou Zhishan , Lu Hui , Zhang Wenliang , Li Yiming , He Yi , Lin Huancai , Zhao Wei , Yu Dongsheng , Zeng Binghui 
Published: March 2023

Background:
Analysis of emerging data shows that miRNAs, including miR-155, play important roles in tumorigenesis. Several studies have indicated that miR-155 and MIR155HG polymorphisms may be related to cancer risk, but the association was controversial. Therefore, we conducted this first-reported comprehensive meta-analysis of the association of miR-155 and MIR155HG polymorphisms with cancer risk.

Materials and Methods:
We searched several databases, including PubMed, Embase, and Web of Science, to identify the eligible studies reporting the association of miR-155 and MIR155HG polymorphisms with cancer risk. We calculated the pooled odds ratios (ORs) and 95% confidence intervals (CIs) to analyze the association. Stata software (version 16.0) was used to analyze the data we collected.

Results:
After being carefully and strictly screened, eight articles reporting on six common single-nucleotide polymorphisms consisting of 6184 cases and 6896 controls were included in this meta-analysis. The six polymorphisms included were rs767649 (T>A), rs928883 (A>G), rs2829803 (G>A), rs1893650 (T>C), rs4143370 (G>C), and rs12482371 (T>C). Our results showed that, in the overall analysis, heterozygotes increased cancer risk, with a marginal P value, compared with wild-type (OR = 1.06, 95% CI = 1.00–1.12, P = 0.062). Subsequent analyses showed that only rs767649 was associated with an increased risk of non-small-cell lung cancer (NSCLC) in an allele model (T vs. A: OR = 1.15, 95% CI = 1.04–1.26, P = 0.007), a homozygote model (TT vs. AA: OR = 1.31, 95% CI = 1.06–1.60, P = 0.011), and a recessive model (TT vs. AT + AA: OR = 1.30, 95% CI = 1.08–1.55, P = 0.005).

Conclusion:
The present meta-analysis indicates that the rs767649 polymorphism might be a potential factor for NSCLC risk; however, more studies should be conducted to confirm these findings.
A Comparative Study on Predictive Validity of Modified Shock Index, Shock Index, and Age Shock Index in Predicting the Need for Mechanical Ventilation among Sepsis Patients in a Tertiary Care Hospital
Authors: Prasad K. J. Devendra , Bindu K. C. Hima , Abhinov T. , Moorthy Krishna , K Rajesh 
Published: March 2023

Introduction:
The shock index (SI), modified shock index (MSI), and age multiplied by SI (ASI) are used to assess the severity of shock. They are also used to predict the mortality of trauma patients, but their validity for sepsis patients is controversial. The aim of this study is to assess the predictive value of the SI, MSI, and ASI in predicting the need for mechanical ventilation after 24 h of admission among sepsis patients.

Methods:
A prospective observational study was conducted in a tertiary care teaching hospital. Patients with sepsis (235) diagnosed based on systemic inflammatory response syndrome criteria and quick sequential organ failure assessment were included in the study. The need for mechanical ventilation after 24 h is the outcome variables MSI, SI, and ASI were considered as predictor variables. The utility of MSI, SI, and ASI in predicting mechanical ventilation was assessed by receiver operative curve analysis. Data were analyzed using coGuide.

Results:
Among the study population, the mean age was 56.12 ± 17.28 years. MSI value at the time of disposition from the emergency room had good predictive validity in predicting mechanical ventilation after 24 h, as indicated by the area under the curve (AUC) of 0.81 (P < 0.001), SI and ASI had fair predictive validity for mechanical ventilation as indicated by AUC (0.78, P < 0.001) and (0.802, P < 0.001), respectively.

Conclusion:
SI had better sensitivity (78.57%) and specificity (77.07%) compared to ASI and MSI in predicting the need for mechanical ventilation after 24 h in sepsis patients admitted to intensive care units.
Relationship between Incontinence and Disease Severity in Patients Transported by Ambulance
Authors: Hamada Michika , Takeuchi Ikuto , Muramatsu Ken-Ichi , Nagasawa Hiroki , Ohsaka Hiromichi , Ishikawa Kouhei , Yanagawa Youichi 
Published: March 2023

Introduction:
Retrospectively investigated this relationship using data from Shimoda Fire Department.

Methods:
We investigated patients who were transported by Shimoda Fire Department from January 2019 to December 2021. The participants were divided into groups based on the existence of incontinence at the scene or not (Incontinence [+] and Incontinence [−]). We compared the variables mentioned above between these groups.

Results:
There were 499 cases with incontinence and 8241 cases without incontinence. There were no significant differences between the two groups with respect to weather and wind speed. The average age, percentage of male patients, percentage of cases in the winter season, rate of collapse at home, scene time, rate of endogenous disease, disease severity, and mortality rate in the incontinence (+) group were significantly greater in comparison to the incontinence (−) group, whereas the average temperature in the incontinence (+) group was significantly lower than that in the incontinence (−) group. Regarding the rates of incontinence of each disease, neurologic, infectious, endocrinal disease, dehydration, suffocation, and cardiac arrest at the scene had more than twice the rate of incontinence in other conditions.

Conclusions:
This is the first study to report that patients with incontinence at the scene tended to be older, showed a male predominance, severe disease, high mortality, and required a long scene time in comparison to patients without incontinence. Prehospital care providers should therefore check for incontinence when evaluating patients.
Pattern of Presentation and Outcome of Adult Patients with Abdominal Trauma – A 7-Year Retrospective Study in a Nigerian Tertiary Hospital
Authors: Adenuga Adedire Timilehin , Adeyeye Ademola 
Published: March 2023

Introduction:
Abdominal trauma is a major cause of morbidity and mortality in low- and middle-income countries. There is a paucity of trauma data in this region and this study aimed to show the pattern of presentation and outcome of patients with abdominal trauma at a North-Central Nigerian Teaching Hospital.

Methods:
This was a retrospective, observational study of patients with abdominal trauma who presented at the University of Ilorin Teaching Hospital from January 2013 to December 2019. Patients with clinical and/or radiological evidence of abdominal trauma were identified, and data extracted and analyzed.

Results:
A total of 87 patients were included in the study. There were 73 males and 14 females (5.2:1) with a mean age of 34.2 years. Blunt abdominal injury occurred in 53 (61%) patients with 10 patients (11%) having concomitant extra-abdominal injuries. A total of 105 abdominal organ injuries occurred in 87 patients with the small bowel being the most frequently injured organ in penetrating trauma, while in blunt abdominal injury, the spleen was most commonly injured. A total of 70 patients (80.5%) had emergency abdominal surgery with a morbidity rate of 38.6% and negative laparotomy rate of 2.9%. There were 15 deaths in the period accounting for 17% of patients with sepsis as the most common cause of death (66%). Shock at presentation, late presentation >12 h, need for perioperative intensive care unit admission, and repeat surgery were associated with a higher risk of mortality (P < 0.05).

Conclusion:
Abdominal trauma in this setting is associated with a significant amount of morbidity and mortality. Typical patients present late and with poor physiologic parameters often resulting in an undesirable outcome. There should be steps targeted at preventive policies focused on reducing the incidence of road traffic crashes, terrorism, and violent crimes as well as improving health care infrastructure to cater to this specific group of patients.
A Comparative Analysis of Thoracic Ultrasound with Radiography in the Detection of Lung Pathologies Among Patients in Intensive Care Units
Authors: Obande Ogenyi , Akinmoladun Janet Adetinuke , Osinaike B. Babatunde , Agunloye M. Atinuke , Ogunseyinde O. Ayotunde 
Published: March 2023

Introduction:
Chest X-ray (CXR) is a common study in intensive care unit (ICU) and to a lesser extent, chest computed tomography. Both studies use ionizing radiation. Bedside ultrasound (US) avoids exposure to ionizing radiation or potentially hazardous movement of the patient.

Materials and Methods:
This was a prospective, cross-sectional, comparative study of B-mode thoracic ultrasound (TUS) with CXR in the detection of lung pathologies in ICU patients. M-mode was further used in suspected pneumothorax (PTX). Patients with clinical suspicion or diagnosis of lung pathologies were recruited. CXR with portable machine and TUS using mobile US machine were done.

Results:
Seventy patients were recruited; 38 (54.3%) were female. Pleural effusion was detected in 17 patients, 15 (88.2%) by TUS and 11 (64.7%) by CXR. TUS and CXR detected lung consolidations in 11 (84.6%) and 9 (69.2%) patients, respectively. PTX was seen by CXR in 6 (85.7%) patients and 5 (71.4%) were seen on TUS. Both modalities diagnosed equal numbers of alveolar and interstitial edemas.

Conclusion:
Overall, there was no statistically significant difference in imaging diagnosis of lung pathologies by TUS and CXR. However, TUS detected more pleural effusion and consolidations; hence, it may be considered an alternative in the management of pediatric patients and pregnant women to avoid ionizing radiation.
Uncovering biomarkers for potential therapeutic targeting for COVID-19-related acute kidney injury: A bioinformatic approach
Authors: Gong Rui , Long Gangyu , Wang Qian , Wang Qiongya , Huang Chaolin , Zhang Dingyu 
Published: March 2023

ABSTRACT

Objective:
The Coronavirus Disease 2019 (COVID-19) is a recently-emerging infectious disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV2), posing a significant threat to public health around the world. In patients with COVID-19, acute kidney injury (AKI) is a common complication associated with poor prognoses. We analyzed co-expressed genes to explore relationships between SARS-CoV2 infection and AKI, and revealed potential biomarkers and therapeutic targets of the COVID-19-associated AKI (COVID-19-AKI).

Methods:
We utilized the GSE147507 and GSE139061 datasets from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) in SARS-CoV-2 infection and AKI, respectively. This was followed by analyzing protein-protein interaction networks, Gene Ontology, and pathway enrichment to uncover the relationship between DEGs. DEGs in common (co-DEGs), as well as corresponding interactive transcription factors (TFs) and microRNAs, were identified from the above results, followed by drug molecules uncovered for managing COVID-19-AKI. Aims: To reveal potential biomarkers and therapeutic targets for COVID-19-AKI by bioinformatic approach.

Results:
We discovered 345 DEGs in the lung and 310 DEGs AKI samples from COVID-19 patients, respectively. IFIT1, ISG15, MX1, IFIT3, and IFIT2 were involved in SARS-CoV-2 pulmonary infection, while hub genes such as RPL23, EIF4A1, RPS8, RPL13, and UPF2 were associated with AKI. We further derived co-DEGs including ERRFI1, KLK10, NR4A1, PODXL, RASGEF1C, RNU11, SNORA12, SNORA74B, and VTRNA1-1 coupled with their predicted transcription factors, including BACH2, HNF4A, MYC, and microRNAs containing miR-637, miR-542-3p, and miR-224. These targets may correlate with COVID-19-AKI, for which candidate drugs were identified.

Conclusions:
ERRFI1, KLK10, NR4A1, PODXL, RASGEF1C, RNU11, SNORA12, SNORA74B, and VTRNA1-1 may be associated with COVID-19-AKI and serve as novel markers.
Tele-psychotherapy practice in India: Ethical and professional concerns
Authors: Singh Shweta , Garaya Amandeep Singh 
Published: March 2023

Abstract
Tele-psychotherapy is especially prominent in times when access to in-person psychotherapy sessions is restricted. For a developing country like India there is a prominent call on mental health care resources on top of the existing mental health treatment gap. The current scenario may have significant implications for telephonic psychotherapy in India. It can be viewed as a reminder to direct changes in mental health laws, training, and research regarding delivering telephonic and online psychotherapy services in India. These changes will need to be supplemented with progress in the design and implementation of research studies to ascertain the efficacy, equivalence, and cost-effectiveness of offering psychotherapy over the online platforms.
Coping strategies used by adult survivors in the process of recovery from disaster trauma: A narrative review
Authors: Kar Nilamadhab 
Published: March 2023

Abstract

CONTEXT:
The catastrophic stress of disasters and the associated secondary traumas lead to negative mental health outcomes in a great proportion of survivors. A range of coping strategies are used, which may have possible preventive and therapeutic effects related to posttraumatic stress-related manifestation.

AIMS:
It is essential to learn about the coping strategies employed by the survivors following disasters to reflect about their usability as possible intervention methods. This review intended to explore the literature about coping mechanisms used by adult survivors of the disasters.

METHODS:
Comprehensive literature search and review.

RESULTS:
It was observed that various coping strategies have been reported, along with posttraumatic mental health conditions. Many of the coping strategies differ in different cultures and other demographic groups. Some articles have made reference to the effectiveness of the coping strategies. It has been observed that the ways coping can be facilitated at different levels. These observations may help in designing culture-specific, pre- and post-disaster management programs for effective and holistic recovery of the survivors.

CONCLUSION:
There is a need to understand the coping mechanisms in different cultures and the factors associated with the resilience in the face of the massive trauma of disasters. It is probable that these could be instrumental in recovery process and expected to help in supporting disaster survivors.