bluuebunny/crossref_metadata_2025_split huggingface dataset:
A causal effects of gut microbiota in the development of migraine
Authors: He Qiang , Wang Wenjing , Xiong Yang , Tao Chuanyuan , Ma Lu , Ma Junpeng , You Chao ,   The International Headache Genetics Consortium, Anttila Verneri , Artto Ville , Belin Andrea C. , Bjornsdottir Anna , Bjornsdottir Gyda , Boomsma Dorret I. , Børte Sigrid , Chalmer Mona A. , Chasman Daniel I. , Cormand Bru , Cuenca-Leon Ester , Davey-Smith George , de Boer Irene , Dichgans Martin , Esko Tonu , Freilinger Tobias , Gormley Padhraig , Griffiths Lyn R. , Hämäläinen Eija , Hansen Thomas F. , Harder Aster V. E. , Hautakangas Heidi , Hiekkala Marjo , Hrafnsdottir Maria G. , Ikram M. Arfan , Järvelin Marjo-Riitta , Kajanne Risto , Kallela Mikko , Kaprio Jaakko , Kaunisto Mari , Kogelman Lisette J. A. , Kristoffersen Espen S. , Kubisch Christian , Kurki Mitja , Kurth Tobias , Launer Lenore , Lehtimäki Terho , Lessel Davor , Ligthart Lannie , Magnusson Sigurdur H. , Malik Rainer , Müller-Myhsok Bertram , Northover Carrie , Nyholt Dale R. , Olesen Jes , Palotie Aarno , Palta Priit , Pedersen Linda M. , Pedersen Nancy , Pirinen Matti , Posthuma Danielle , Pozo-Rosich Patricia , Pressman Alice , Raitakari Olli , Ran Caroline , Sigurdardottir Gudrun R. , Stefansson Hreinn , Stefansson Kari , Sveinsson Olafur A. , Terwindt Gisela M. , Thorgeirsson Thorgeir E. , van den Maagdenberg Arn M. J. M. , van Duijn Cornelia , Wessman Maija , Winsvold Bendik S. , Zwart John-Anker 
Published: July 2023

Abstract
Background
The causal association between the gut microbiome and the development of migraine and its subtypes remains unclear.

Methods
The single nucleotide polymorphisms concerning gut microbiome were retrieved from the gene-wide association study (GWAS) of the MiBioGen consortium. The summary statistics datasets of migraine, migraine with aura (MA), and migraine without aura (MO) were obtained from the GWAS meta-analysis of the International Headache Genetics Consortium (IHGC) and FinnGen consortium. Inverse variance weighting (IVW) was used as the primary method, complemented by sensitivity analyses for pleiotropy and increasing robustness.

Results
In IHGC datasets, ten, five, and nine bacterial taxa were found to have a causal association with migraine, MA, and MO, respectively, (IVW, all P < 0.05). Genus.Coprococcus3 and genus.Anaerotruncus were validated in FinnGen datasets. Nine, twelve, and seven bacterial entities were identified for migraine, MA, and MO, respectively. The causal association still exists in family.Bifidobacteriaceae and order.Bifidobacteriales for migraine and MO after FDR correction. The heterogeneity and pleiotropy analyses confirmed the robustness of IVW results.

Conclusion
Our study demonstrates that gut microbiomes may exert causal effects on migraine, MA, and MO. We provide novel evidence for the dysfunction of the gut-brain axis on migraine. Future study is required to verify the relationship between gut microbiome and the risk of migraine and its subtypes and illustrate the underlying mechanism between them.
Urinary bladder melanosis with complete resolution case report
Authors: Mateescu Daniela I. , Dahir Hamdi , Goel Amit , Ray Eleanor , Madaan Sanjeev 
Published: July 2023

Abstract
Background
Melanosis of the bladder is a rare condition characterized by multifocal, diffuse melanin pigmentation of the urothelial mucosa or lamina propria without any proliferation of melanocytes. Less than 25 cases have been reported so far of which only four patients had concurrent urothelial carcinoma.

Case presentation
We are reporting a case of melanosis of the bladder in a 58-year-old female associated with pTaG1 transitional urothelial cell carcinoma (TCC) of the bladder who presented with symptoms of recurrent urinary tract infection (UTI). Cystoscopy and biopsy results confirmed benign melanosis.

Conclusion
Our case is unique because bladder melanosis associated low grade TCC with complete resolution of both conditions within one year.
Ameliorative potential of the quercetin on lead-induced testicular damage: morphohistometric and biochemical analysis
Authors: Mustafa Hesham N. 
Published: July 2023

Abstract
Background
Quercetin, a naturally occurring flavonoid known for its potent antioxidant properties, has been investigated for its potential in counteracting the harmful effects of lead (Pb) toxicity, which induces apoptosis and oxidative damage in various human tissues. This study aims to assess the reparative effects of quercetin on lead-induced testicular damage.

Methods
Four groups, each comprising ten adult male albino rats, were randomly assigned as follows: Quercetin group, Pb group, Pb + Quercetin group, and control group. All treatments were administered orally via gavage daily for a duration of 30 days. Evaluation of sex hormone levels (serum testosterone, FSH, and LH), cytokines and inflammatory mediators (IL-1β, TNF-α, MCP-1), lead concentration, oxidative and antioxidant stress markers (superoxide anion [O2−], MDA, SOD, CAT, GSH), and sperm characteristics were carried out.

Results
The results demonstrated a significant decline in sex hormones and antioxidants, accompanied by an increase in lead concentrations, cytokines, inflammatory mediators, and oxidative stress indicators (O2−, MDA), while SOD, CAT, and GSH levels were reduced. The Pb-intoxicated group exhibited a substantial increase in dead and abnormal sperm, along with significant reductions in sperm concentration and motility. Morphometrically, a marked decrease was observed in spermatogonia, primary spermatocytes, spermatids, and sertoli cells per seminiferous tubule, as well as epithelial height. Furthermore, coadministration of quercetin exhibited notable benefits. It significantly elevated testosterone levels (P < 0.001), testicular SOD, CAT, and GSH activities, while decreasing MDA levels (P < 0.001). Quercetin also mitigated the deleterious effects of lead toxicity on sperm parameters and restored morphometric variations, including epithelial height.

Conclusions
Quercetin supplementation alongside lead exposure showed a potential for ameliorating degenerative changes caused by lead toxicity in the testicles. This cotreatment effectively reduced oxidative stress, cytokine levels, inflammatory mediators, and restored biochemical alterations, thereby improving morphometric parameters.
Assessment of androgen receptor expression in breast cancer patients using 18 F-FDG PET/CT radiomics and clinicopathological characteristics
Authors: Jia Tongtong , Lv Qingfu , Zhang Bin , Yu Chunjing , Sang Shibiao , Deng Shengming 
Published: July 2023

Abstract
Objective
In the present study, we mainly aimed to predict the expression of androgen receptor (AR) in breast cancer (BC) patients by combing radiomic features and clinicopathological factors in a non-invasive machine learning way.

Materials and methods
A total of 48 BC patients, who were initially diagnosed by 18F-FDG PET/CT, were retrospectively enrolled in this study. LIFEx software was used to extract radiomic features based on PET and CT data. The most useful predictive features were selected by the LASSO (least absolute shrinkage and selection operator) regression and t-test. Radiomic signatures and clinicopathologic characteristics were incorporated to develop a prediction model using multivariable logistic regression analysis. The receiver operating characteristic (ROC) curve, Hosmer-Lemeshow (H-L) test, and decision curve analysis (DCA) were conducted to assess the predictive efficiency of the model.

Results
In the univariate analysis, the metabolic tumor volume (MTV) was significantly correlated with the expression of AR in BC patients (p < 0.05). However, there only existed feeble correlations between estrogen receptor (ER), progesterone receptor (PR), and AR status (p = 0.127, p = 0.061, respectively). Based on the binary logistic regression method, MTV, SHAPE_SphericityCT (CT Sphericity from SHAPE), and GLCM_ContrastCT (CT Contrast from grey-level co-occurrence matrix) were included in the prediction model for AR expression. Among them, GLCM_ContrastCT was an independent predictor of AR status (OR = 9.00, p = 0.018). The area under the curve (AUC) of ROC in this model was 0.832. The p-value of the H-L test was beyond 0.05.

Conclusions
A prediction model combining radiomic features and clinicopathological characteristics could be a promising approach to predict the expression of AR and noninvasively screen the BC patients who could benefit from anti-AR regimens.
Parents’ experiences of life after medicalised conception: a thematic meta-synthesis of the qualitative literature
Authors: Foyston Z. , Higgins L. , Smith D. M. , Wittkowski A. 
Published: July 2023

Abstract
Background
Medicalised Conception (MAC) assists many couples to achieve pregnancy worldwide. As the impact of MAC has been linked to increased pregnancy-specific anxiety and parenting difficulties, this review aimed to explore parental experiences of pregnancy and early parenting following MAC, identifying parents’ psychological, social and health needs.

Method
Five databases were searched systematically from inception to March 2023. Identified articles were screened for eligibility against the inclusion criteria and the results were analysed using thematic synthesis. The Critical Appraisal Skills checklist was employed to appraise methodological quality.

Results
Twenty qualitative studies, drawing on a total of 19 participant samples, were included in this review, most with samples with history of subfertility. The findings were synthesised into three main themes (consisting of seven subthemes): 1) The vulnerable parent: fear, doubt, uncertainty, 2) the stark realisation of the parental dream, 3) psychosocial needs and support. Parents lacked a sense of safety during pregnancy and reported acting protectively both antenatally and postnatally. Furthermore, their identity transition was complex and non-linear, influenced by sociocultural context.

Conclusions
Considerable unmet psychosocial needs were identified including the potential for anxiety in pregnancy, the possibility of feeling excluded and marginalised, and a reluctance to share distress and experiences with healthcare professionals. These findings suggest a need for consistent, holistic care, integrating psychological services.
MicroRNA-191 regulates oral squamous cell carcinoma cells growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway
Authors: Wang Zekun , Guan Wenzhao , Ma Yufeng , Zhou Xuedong , Song Guohua , Wei Jianing , Wang Chenyang 
Published: July 2023

Abstract
Background
Studies have shown that microRNA-191 (miR-191) is involved in the development and progression of a variety of tumors. However, the function and mechanism of miR-191 in oral squamous cell carcinoma (OSCC) have not been clarified.

Methods
The expression level of miR-191 in tumor tissues of patients with primary OSCC and OSCC cell lines were detected using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. OSCC cells were treated with miR-191 enhancers and inhibitors to investigate the effects of elevated or decreased miR-191 expression on OSCC cells proliferation, migration, cell cycle, and tumorigenesis. The target gene of miR-191 in OSCC cells were analyzed by dual-Luciferase assay, and the downstream signaling pathway of the target genes was detected using western blot assay.

Results
The expression of miR-191 was significantly upregulated in OSCC tissues and cell lines. Upregulation of miR-191 promoted proliferation, migration, invasion, and cell cycle progression of OSCC cells, as well as tumor growth in nude mice. Meanwhile, reduced expression of miR-191 inhibited these processes. Phospholipase C delta1 (PLCD1) expression was significantly downregulated, and negatively correlated with the expression of miR-191 in OSCC tissues. Dual-Luciferase assays showed that miR-191-5p could bind to PLCD1 mRNA and regulate PLCD1 protein expression. Western blot assay showed that the miR-191 regulated the expression of β-catenin and its downstream gene through targeting PLCD1.

Conclusion
MicroRNA-191 regulates oral squamous cell carcinoma cells growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway. Thus, miR-191 may serve as a potential target for the treatment of OSCC.
Efficacy and safety of Xian-Lian-Jie-Du optimization decoction as an adjuvant treatment for prevention of recurrence in patients with stage IIIB/IIIC colon cancer: study protocol for a multicentre, randomized controlled trial
Authors: Geng Xuechen , Wang Ziqiang , Feng Li , Gu Yanhong , Wang Renjie , Yao Qinghua , Xu Yangxian , Wu Jianyu , Jiang Zhiwei , Chen Kai , Hu Wenwei , Tang Dongxin , Huo Jiege , Li Ling , Bu Qianqian , Zhao Shuoqi , Zhang Bei , Cheng Haibo 
Published: July 2023

AbstractIntroductionColon cancer remains one of the most prevalent cancers worldwide. Unfortunately, there are no recognized and effective therapeutic strategies to prevent tumor recurrence after radical resection and chemotherapy, and the disease-free survival (DFS) in patients with stage IIIB or IIIC disease remains unsatisfactory. Xian-Lian-Jie-Du optimization decoction (XLJDOD) is a Chinese herbal medicine (CHM) empirical prescription, which has been validated experimentally and clinically that could inhibit the progression of colorectal cancer and ameliorate the symptoms. The purpose of this study is to evaluate the efficacy and safety of XLJDOD in prevention of recurrence of colon cancer.MethodsThis study is a multi-center, double-blind, randomized, placebo-controlled trial conducted at 13 hospitals of China. Following the completion of surgery and adjuvant 5- fluorouracil-based chemotherapy, a total of 730 subjects with stage IIIB or IIIC colon cancer will be randomized in a 1:1 ratio to an intervention group (n = 365; XLJDOD compound granule) and a control group (n = 365; Placebo). Patients will receive 6-month treatments and be followed up with 3 monthly assessments for 2 years. The primary outcome is 2-year DFS rate and the secondary outcomes are 1, 2-year relapse rate (RR), overall survival (OS) and quality of life (QoL). Safety outcomes such as adverse events will be also assessed. A small number of subgroup analysis will be carried out to explore the heterogeneity of effects of XLJDOD.DiscussionThe outcomes from this randomized controlled trial will provide objective evidences to evaluate XLJDOD’s role as an adjuvant treatment in colon cancer.Trial registrationwww.ClinicalTrials.gov, identifier: NCT05709249. Registered on 31 Jan 2023.
Dental students’ perception of their educational environment in relation to their satisfaction with dentistry major: a cross-sectional study
Authors: Gil Yoon Min , Hong Ji Soo , Ban Ju Ly , Kwon Jae-Sung , Lee Jae-Il 
Published: July 2023

Abstract
Background
Students’ perception of their educational environment and satisfaction with their major can reveal the extent of their readiness to practice their profession after graduation. This study aimed to evaluate dental students’ perception of their educational environment and satisfaction with their major in dentistry, as well as the relationship between these two factors.

Methods
An online survey was conducted in 2022 among first- to fourth-year students across 11 dental schools in Korea. The Dundee Ready Education Environment Measure (DREEM) and Academic Major Satisfaction Scale (AMSS) were used to measure the students’ perception of the educational environment and satisfaction with their major in dentistry, respectively.

Results
A total of 539 students participated in the survey (response rate = 18.1%). The overall mean scores of the DREEM and AMSS were 125.03 (maximum score 200) and 22.01 (maximum score 30), respectively. Fourth-year students had the lowest scores in the overall DREEM, DREEM subscales (excluding students’ perceptions of atmosphere), and AMSS. The overall DREEM scores and all DREEM subscales showed statistically significant positive and moderate correlations with AMSS (p < 0.001).

Conclusion
Using the DREEM, we identified areas that need improvement and the academic year (fourth year) that require proactive support. Considering the positive correlation between all DREEM subscales and the AMSS, measures to comprehensively improve the educational environment are needed to improve dental students’ satisfaction with their major.
Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy
Authors: Torricelli F , Donati B , Reggiani F , Manicardi V , Piana S , Valli R , Lococo F , Ciarrocchi Alessia 
Published: July 2023

AbstractBackgroundMalignant Pleural Mesothelioma (MPM) is a dreadful disease escaping the classical genetic model of cancer evolution and characterized by wide heterogeneity and transcriptional plasticity. Clinical evolution of MPM is marked by a progressive transdifferentiation that converts well differentiated epithelioid (E) cells into undifferentiated and pleomorphic sarcomatoid (S) phenotypes. Catching the way this transition takes place is necessary to understand how MPM develops and progresses and it is mandatory to improve patients’ management and life expectancy. Bulk transcriptomic approaches, while providing a significant overview, failed to resolve the timing of this evolution and to identify the hierarchy of molecular events through which this transition takes place.MethodsWe applied a spatially resolved, high-dimensional transcriptomic approach to study MPM morphological evolution. 139 regions across 8 biphasic MPMs (B-MPMs) were profiled using the GeoMx™Digital Spatial Profiler to reconstruct the positional context of transcriptional activities and the spatial topology of MPM cells interactions. Validation was conducted on an independent large cohort of 84 MPMs by targeted digital barcoding analysis.ResultsOur results demonstrated the existence of a complex circular ecosystem in which, within a strong asbestos-driven inflammatory environment, MPM and immune cells affect each other to support S-transdifferentiation. We also showed that TGFB1 polarized M2-Tumor Associated Macrophages foster immune evasion and that TGFB1 expression correlates with reduced survival probability.ConclusionsBesides providing crucial insights into the multidimensional interactions governing MPM clinical evolution, these results open new perspectives to improve the use of immunotherapy in this disease.
Successful treatment of lupus anticoagulant hypoprothrombinemia syndrome with rituximab
Authors: Nusrat Sanober , Tewari Sayani , Khan Osman 
Published: July 2023

AbstractLupus anticoagulant-hypoprothrombinemia syndrome (LAHPS) is a rare acquired bleeding disorder secondary to development of antibodies against prothrombin protein, in the presence of antiphospholipid antibodies. We describe the case of a 13-year-old girl who presented with severe menorrhagia and symptomatic anemia. Labs indicated anemia, thrombocytopenia, elevated PT and aPTT, high-titer inhibitor on mixing studies, positive ANA and anti-dsDNA antibodies, along with a triple-positive antiphospholipid antibody panel. Given additional systemic manifestations, systemic lupus erythematosus was diagnosed. High dose steroids and hydroxychloroquine subsequently started. Her clinical course was complicated by femoral deep venous thrombosis and post renal biopsy retroperitoneal hematoma. Further workup revealed low prothrombin level and the diagnosis of lupus anticoagulant hypoprothrombinemia syndrome. In view of suboptimal response to initial immunosuppressive therapy, rituximab was added to her regimen, leading to an improvement in clinical symptoms and resolution of hypoprothrombinemia. She remains recurrence free 5 years from the event.
Wildfire national carbon accounting: how natural and anthropogenic landscape fires emissions are treated in the 2020 Australian government greenhouse gas accounts report to the UNFCCC
Authors: Bowman David MJS , Williamson Grant J. , Ndalila Mercy , Roxburgh Stephen H. , Suitor Shaun , Keenan Rodney J. 
Published: July 2023

AbstractGreenhouse gas (GHG) accounting of emissions from land use, land-use change, and forestry necessarily involves consideration of landscape fire. This is of particular importance for Australia given that natural and human fire is a common occurrence, and many ecosystems are adapted to fire, and require periodic burning for plant regeneration and ecological health. Landscape fire takes many forms, can be started by humans or by lightning, and can be managed or uncontrolled. We briefly review the underlying logic of greenhouse gas accounting involving landscape fire in the 2020 Australian Government GHG inventory report. The treatment of wildfire that Australia chooses to enact under the internationally agreed guidelines is based on two core assumptions (a) that effects of natural and anthropogenic fire in Australian vegetation carbon stocks are transient and they return to the pre-fire level relatively quickly, and (b) that historically and geographically anomalous wildfires in forests should be excluded from national anthropogenic emission estimates because they are beyond human control. It is now widely accepted that anthropogenic climate change is contributing to increased frequency and severity of forest fires in Australia, therefore challenging assumptions about the human agency in fire-related GHG emissions and carbon balance. Currently, the national inventory focuses on forest fires; we suggest national greenhouse gas accounting needs to provide a more detailed reporting of vegetation fires including: (a) more detailed mapping of fire severity patterns; (b) more comprehensive emission factors; (c) better growth and recovery models from different vegetation types; (d) improved understanding how fires of different severities affect carbon stocks; and (e) improved analysis of the human agency behind the causes of emissions, including ignition types and fire-weather conditions. This more comprehensive accounting of carbon emissions would provide greater incentives to improve fire management practices that reduce the frequency, severity, and extent of uncontrolled landscape fires.