bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análisis de correspondencia múltiple entre el tamaño de las empresas y su participación económica en el Estado de México
Authors: Puebla Maldonado Armando 
Published: March 2023

La presente investigación tiene como propósito el análisis económico regional que coadyuve en el diagnóstico de la planeación sectorial del Estado de México. Para cumplir con este propósito se buscó determinar el nivel de asociación entre el tamaño de las unidades económicas y un conjunto de variables representativas de la actividad económica. Se partió de la metodología de minería de datos para aplicar de manera cuantitativa y descriptiva la metodología del Análisis de Correspondencia Múltiple (ACM). Los resultados sugieren, que hay un nivel de asociación positiva moderada alrededor del 50 % en dos de sus indicadores: El coeficiente de asociación de Pearson es de 0.4685 y el de Mathews con 0.5303. Sin embargo, en los indicadores V de Cramer y de Inercial Total fueron correlaciones positivas débiles respectivamente de 0.3062 y 0.2812. Ante esta situación se propuso como medida alternativa el análisis discriminante y el de varianza, los cuales arrojaron que las microempresas están más asociadas con el Personal Ocupado Total (POT) y la Producción Bruta Total (PBT). Por su parte, el Valor Agregado Censal Bruto (VACB) e Ingreso por Suministros de Bienes y Servicios (ISBB) con medianas y grandes empresas. Asimismo, la prueba Chi-Cuadrado, además de que comprobó que los datos son estadísticamente significativos al 95 %, también apoyo la hipótesis de una asociación entre el tamaño de las unidades económicas y las variables de Participación Económica. Los resultados abonan a que las políticas públicas deberían considerar la heterogeneidad de la planta productiva y su participación económica.
Pelatihan Penggunaan Educandy Sebagai Game Edukatif Di Sd Muhammadiyah 08 Plus Jakarta Timur
Authors: Rahmanto Muhammad Arifin , Bunyamin Bunyamin , Noviyanti Kiky 
Published: March 2023

Dalam Proses Pembelajaran, tentunya seorang guru harus mempersiapkan media agar para peserta didik mudah memahami materi yang dapat meningkatkan hasil belajar mereka dan tercapainya tujuan dalam pendidikan. Tantangan seorang guru adalah menciptakan suasana belajar yang tidak membosankan agar pada saat proses pembelajaran berlangsung mampu memitigasi peserta didik yang kurang melibatkan diri secara aktif dan cenderung pasif. Begitupun guru harus memahami bahwa untuk mengevaluasi pembelajaran tidak selalu dengan cara ujian atau menjawab latihan soal yang memiliki kesan monoton dan membosankan. Umumnya, pengevaluasian terhadap peserta didik berbentuk soal PG dan Esai saja, sehingga bentuk evaluasi yang digunakan kurang menarik para peserta didik. Dari permasalahan diatas, Solusi yang ditawarkan adalah dengan memberikan Pelatihan penggunaan Educandy sebagai Game Edukasi di SD Muhammadiyah 8 Plus Jakarta. Aplikasi ini merupakam game edukasi yang dapat membuat pembelajaran menjadi bervariasi dan menyenangkan. Sehingga, peserta didik tidak merasa bosan dan termotivasi untuk belajar lebih giat lagi. Pelatihan ini bertujuan untuk meningkatkan kemampuan guru-guru dalam mengajarkan suatu materi terhadap peserta didik dan mengevaluasinya. Sehingga, pembelajaran akan berjalan lebih efektif dan menjadikan guru lebih professional. Kegiatan pelatihan ini, nantinya akan berlangsung secara Offline lebih difokuskan kepada seorang guru dengan metode diskusi panel dan praktek langsung dalam pemanfaatan Game Educandy.Kata Kunci: Media, Educandy, evaluasi
Inflammation in Major Depressive Disorder Patients with and without Attempted Suicide
Authors: TAŞCI Gülay , ÖZSOY Filiz 
Published: March 2023

Objective: This study aimed to examine Major Depressive Disorder (MDD) patients with or without attempted suicide history for inflammation parameters.
Methods: The study included 287 participants, 187 had MDD diagnosis (87 patients with a suicide attempt, 100 patients without suicide history) and 100 healthy. All participants were subjected to Beck Depression Inventory (BDI).
Results: Mean BDI score of MDD patients with suicide attempts was higher than the other two groups (p
Throughout in vitro first spermatogenic wave: Next-generation sequencing gene expression patterns of fresh and cryopreserved prepubertal mice testicular tissue explants
Authors: Dumont Ludovic , Lopez Maestre Hélène , Chalmel Frédéric , Huber Louise , Rives-Feraille Aurélie , Moutard Laura , Bateux Frédérique , Rondanino Christine , Rives Nathalie 
Published: March 2023

IntroductionSuitable cryopreservation procedures of pre-pubertal testicular tissue associated with efficient culture conditions are crucial in the fields of fertility preservation and restoration. In vitro spermatogenesis remains a challenging technical procedure to undergo a complete spermatogenesis.The number of haploid cells and more specifically the spermatic yield produced in vitro in mice is still extremely low compared to age-matched in vivo controls and this procedure has never yet been successfully transferred to humans.MethodsTo evaluate the impact of in vitro culture and freezing procedure, pre-pubertal testicular mice testes were directly cultured until day 4 (D4), D16 and D30 or cryopreserved by controlled slow freezing then cultured until D30. Testes composed of a panel of 6.5 dpp (days postpartum), 10.5 dpp, 22.5 dpp, and 36.5 dpp mice were used as in vivo controls. Testicular tissues were assessed by histological (HES) and immunofluorescence (stimulated by retinoic acid gene 8, STRA8) analyses. Moreover, a detailed transcriptome evaluation study has been carried out to study the gene expression patterns throughout the first in vitro spermatogenic wave.ResultsTranscriptomic analyses reveal that cultured tissues expression profiles are almost comparable between D16 and D30; highlighting an abnormal kinetic throughout the second half of the first spermatogenesis during in vitro cultures. In addition, testicular explants have shown dysregulation of their transcriptomic profile compared to controls with genes related to inflammation response, insulin-like growth factor and genes involved in steroidogenesis.DiscussionThe present work first shows that cryopreservation had very little impact on gene expression in testicular tissue, either directly after thawing or after 30 days in culture. Transcriptomic analysis of testis tissue samples is highly informative due to the large number of expressed genes and identified isoforms. This study provides a very valuable basis for future studies concerning in vitro spermatogenesis in mice.
Research on the application of improved NSGA-II in the structure design of wind turbine blade spar cap
Authors: Chen Cheng , Wang Long , Niu Muhua 
Published: March 2023

Introduction: The blade is one of the most important parts of a wind turbine. Blade design is a complicated process involving many design links and mutual couplings. To efficiently obtain the Pareto Front and optimal blade, a novel methodology combined the improved NSGA-II with Thin-Walled Beam Theory and Classic Laminated Theory has been developed.Methods: In the optimization study, the design parameters are the thickness distribution of the spar cap and a parametric model based on blade production process is developed. The objective function is on the minimization of mass and the maximization of flap-wise stiffness. As a practical engineering application research, a 15 MW wind turbine IEA-15-240-RWT developed by NREL is used as a reference model.Results: Laws on the number of variables, blade profiles, design loads and natural frequencies present in the design have been studied through Pareto fronts obtained from multi-objective optimization results.Discussion: These laws have important implications for practical engineering design work. Meanwhile, the results show that the new methodology is effective for blade structure design, and that this approach can be easily extended to study the design of influence laws for more design parameters or more complex blade design problems.
Impacts of vitrification on the transcriptome of human ovarian tissue in patients with gynecological cancer
Authors: Gu Ruihuan , Ge Naidong , Huang Bin , Fu Jing , Zhang Ying , Wang Ningyi , Xu Yan , Li Lu , Peng Xiandong , Zou Yaoyu , Sun Yijuan , Sun Xiaoxi 
Published: March 2023

Objective: This study investigated the effects of a vitrification/warming procedure on the mRNA transcriptome of human ovarian tissues.Design: Human ovarian tissues were collected and processed through vitrification (T-group) and then subjected to RNA sequencing (RNA-seq) analysis, HE, TdT-mediated dUTP nick-end labeling (TUNEL), and real-time quantitative PCR, and the results were compared to those of the fresh group (CK).Results: A total of 12 patients, aged 15–36 years old, with a mean anti-Müllerian hormone level of 4.57 ± 3.31 ng/mL were enrolled in this study. According to the HE and TUNEL results, vitrification effectively preserved human ovarian tissue. A total of 452 significantly dysregulated genes (|log2FoldChange| &gt; 1 and p &lt; 0.05) were identified between the CK and T groups. Among these, 329 were upregulated and 123 were downregulated. A total of 372 genes were highly enriched for 43 pathways (p &lt; 0.05), which were mainly related to systemic lupus erythematous, cytokine–cytokine receptor interaction, the TNF signaling pathway, and the MAPK signaling pathway. IL10, AQP7, CCL2, FSTL3, and IRF7 were significantly upregulated (p &lt; 0.01), while IL1RN, FCGBP, VEGFA, ACTA2, and ASPN were significantly downregulated in the T-group (p &lt; 0.05) compared to the CK group, which agreed with the results of the RNA-seq analysis.Conclusion: These results showed (for the first time to the authors’ knowledge) that vitrification can induce changes in mRNA expression in human ovarian tissues. Further molecular studies on human ovarian tissues are required to determine whether altered gene expression could result in any downstream consequences.
Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis
Authors: Wang Yuan , Li Xuan , Gu Shuntong , Fu Junhong 
Published: March 2023

BackgroundAllergic rhinoconjunctivitis (ARC) and allergic rhinitis (AR) are prevalent allergic diseases. People are becoming increasingly aware of the impact of microbial disorders on host immunity and allergic diseases. Studies have demonstrated an association between allergic diseases and the microbiome, but much remains unknown. We assessed changes in the conjunctival microbiome and nasal microbiome in patients with ARC or AR.MethodsConjunctival swabs and nasal swabs were collected from each participant for 16S rRNA amplicon sequencing. Bacterial communities were analyzed.ResultsForty patients with ARC, 20 patients suffering from AR, and 34 healthy controls (HCs) were recruited. This study found the abundance of conjunctival microbiome in patients with ARC or AR was significantly lower than that in HCs. The diversity of conjunctival microbiome in patients with AR was significantly lower than those in the other two groups. There is no significant difference in abundance of nasal microbiome between the three groups. The diversities of nasal microbiome in patients with ARC or AR were significantly lower than that in HCs. We found significant differences in microbiota compositions in patients with ARC or AR compared with those in HCs. However, no significant difference in microbiota compositions was found between patients with ARC and patients with AR. Microbiome functions in the ARC group and AR group were also altered compared with HCs.ConclusionsWe revealed changes in the composition and function of the conjunctival microbiome and nasal microbiome of patients with ARC or AR, which suggests that there is a relationship between allergic conditions and the local microbiome.
Anti-cyclooxygenase, anti-glycation, and anti-skin aging effect of Dendrobium officinale flowers’ aqueous extract and its phytochemical validation in aging
Authors: Zhou Huiji , Zhou Luxian , Li Bo , Yue Rongcai 
Published: March 2023

IntroductionDendrobium officinale Kimura et Migo (D. officinale) , widely called as “life-saving immortal grass” by Chinese folk, is a scarce and endangered species. The edible stems of D. officinale have been extensively studied for active chemical components and various bioactivities. However, few studies have reported the well-being beneficial effects of D. officinale flowers (DOF). Therefore, the present study aimed to investigate the in vitro biological potency of its aqueous extract and screen its active components.MethodsAntioxidant tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the ferric reducing ability of plasma (FRAP), and intracellular reactive oxygen species (ROS) level analyses in primary human epidermal keratinocytes, anti-cyclooxygenase2 (COX-2) assay, anti-glycation assay (both fluorescent AGEs formation in a BSA fructose/glucose system and glycation cell assay), and anti-aging assay (quantification of collagen types I and III, and SA-β-gal staining assay) were conducted to determine the potential biological effects of DOF extracts and its major compounds. Ultra-performance liquid chromatography-electrospray ionisation-quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS/MS) was performed to investigate the composition of DOF extracts. Online antioxidant post-column bioassay tests were applied to rapidly screen major antioxidants in DOF extracts.Results and discussionThe aqueous extract of D. officinale flowers was found to have potential antioxidant capacity, anti-cyclooxygenase2 (COX-2) effect, anti-glycation potency, and anti-aging effects. A total of 34 compounds were identified using UPLC-ESI-QTOF-MS/MS. Online ABTS radical analysis demonstrated that 1-O-caffeoyl-β-D-glucoside, vicenin-2, luteolin-6-C-β-D-xyloside-8-C-β--D-glucoside, quercetin-3-O-sophoroside, rutin, isoquercitrin, and quercetin 3-O-(6″-O-malonyl)-β-D-glucoside are the major potential antioxidants. In addition, all selected 16 compounds exerted significant ABTS radical scavenging ability and effective AGE suppressive activities. However, only certain compounds, such as rutin and isoquercitrin, displayed selective and significant antioxidant abilities, as shown by DPPH and FRAP, as well as potent COX-2 inhibitory capacity, whereas the remaining compounds displayed relatively weak or no effects. This indicates that specific components contributed to different functionalities. Our findings justified that DOF and its active compound targeted related enzymes and highlighted their potential application in anti-aging.
Ankylosing spondylitis and glaucoma in European population: A Mendelian randomization study
Authors: Li Shengjie , Chen Minting , Zhang Qing , Fang Meijin , Xiong Wei , Bai Lang 
Published: March 2023

BackgroundThe relationship between ankylosing spondylitis (AS) and glaucoma in the European population remains unclear. In the present study, we applied a two-sample Mendelian randomization (MR) method to investigate their causal relationship.MethodsMR analysis was conducted to validate the causal associations between AS with glaucoma using summary statistics from the genome-wide association studies of AS (9,069 cases and 13,578 control subjects) and glaucoma (8,591 cases and 210,201 control subjects). The inverse variance weighting method was performed to evaluate the causal relationship. The MR–Egger regression approach was applied to assess pleiotropy, while Cochran’s Q test was used to analyze heterogeneity. Subgroup analysis was performed according to primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG).ResultsThe results of the MR study reveal a risk-increasing causal relationship between AS and glaucoma among European populations (OR = 1.35, 95%CI = 1.16–1.57, P = 8.81 × 10-5). Pleiotropy and heterogeneity were not found in our study. In the subgroup analysis, AS was also causal with POAG (OR = 1.48, 95%CI = 1.17–1.86, P = 8.80 × 10-4) and PACG (OR = 1.91, 95%CI = 1.03–3.51, P = 3.88 × 10-2).ConclusionThe results of the MR analysis suggested a causal relationship between AS and glaucoma in the European population. Further studies are needed to identify the specific mechanism between these two diseases.
Hemorrhage complications in obstetric antiphospholipid syndrome: Risk factors and association with adverse pregnancy outcomes
Authors: Luo Yongjing , Jin Jiayang , Yan Yani , Zhang Mengyao , Hou Lei , Hou Yuke , Pei Qiuyan , Li Chun 
Published: March 2023

BackgroundBleeding complications are recognized as relatively infrequent manifestations of antiphospholipid syndrome (APS), and the safety of antithrombotic therapy during pregnancy is of concern. This study aims to assess the risk factors and possible associations between bleeding complications and adverse pregnancy outcomes (APOs) in patients with APS.MethodsA retrospective cohort study was conducted at the Peking University People’s Hospital. The clinical and immunologic features, bleeding complications, treatment, and pregnancy outcomes of patients with APS were collected. Univariate and multivariate logistic regression analyses were applied to assess the associations between APOs and bleeding complications.ResultsA total of 176 participants with obstetric APS were included in the analysis. There were 66 (37.50%) patients with APS with hemorrhage complications and 86 (48.86%) patients with APS with APOs. Mucocutaneous hemorrhage was associated with APOs including fetal death after 12 weeks [odds ratio (OR) = 10.73, 95% confidence interval (CI): 1.61–71.74, p = 0.014], preterm delivery prior to 34 weeks (OR = 8.30, 95% CI: 2.31–29.84, p = 0.001), and small for gestational age (OR = 4.17, 95% CI: 1.22–14.21, p = 0.023) in univariate logistic regression analyses. It also independently associated with preterm delivery prior to 34 weeks (OR = 40.29, 95% CI: 1.45–1121.32, p = 0.030) in multivariate logistic regression analyses. Receiver operating characteristic (ROC) analysis evaluating the accuracy of these factors for preterm delivery prior to 34 weeks showed that the area under ROC curve was 0.871.ConclusionThe study shows that mucocutaneous hemorrhage may be an indication of the occurrence of APOs in obstetric patients with APS.
Deficient leptin receptor signaling in T cells of human SLE
Authors: Liu Ting , Zheng Ming , Jia Li , Wang Mingyuan , Tang Longhai , Wen Zhenke , Zhang Miaojia , Yuan Fenghong 
Published: March 2023

BackgroundSystemic lupus erythematosus (SLE) is a prototypic autoimmune disease mainly mediated by IgG autoantibody. While follicular helper T (Tfh) cells are crucial for supporting IgG autoantibody generation in human SLE, underlying mechanisms for Tfh cell mal-differentiation remain unclear.MethodsIn total, 129 SLE patients and 37 healthy donors were recruited for this study. Circulating leptin was determined by ELISA from patients with SLE and healthy individuals. CD4 T cells isolated from SLE patients and healthy donors were activated with anti-CD3/CD28 beads under cytokine-unbiased conditions in the presence or absence of recombinant leptin protein, followed by detection for Tfh cell differentiation by quantifying intracellular transcription factor Bcl-6 and cytokine IL-21. AMPK activation was assessed by analyzing phosphor-AMPK using phosflow cytometry and immunoblots. Leptin receptor expression was determined using flow cytometry and its overexpression was achieved by transfection with an expression vector. Humanized SLE chimeras were induced by injecting patients’ immune cells into immune-deficient NSG mice and used for translational studies.ResultsCirculating leptin was elevated in patients with SLE, inversely associated with disease activity. In healthy individuals, leptin efficiently inhibited Tfh cell differentiation through inducing AMPK activation. Meanwhile, leptin receptor deficiency was a feature of CD4 T cells in SLE patients, impairing the inhibitory effect of leptin on the differentiation of Tfh cells. As a result, we observed the coexistence of high circulating leptin and increased Tfh cell frequencies in SLE patients. Accordingly, overexpression of leptin receptor in SLE CD4 T cells abrogated Tfh cell mal-differentiation and IgG anti-dsDNA generation in humanized lupus chimeras.ConclusionLeptin receptor deficiency blocks the inhibitory effect of leptin on SLE Tfh cell differentiation, serving as a promising therapeutic target for lupus management.
WSP from “Nostoc commune” Vauch. suppresses gastric cancer migration via EGFRVIII signaling
Authors: Chen Xiaoxia , Bai Wenqi , Liu Xiangrong , Zhao Jiao , Li Zhiyuan , Li Jianrong , Su Liping , Guan Tao , Sun Ruifang , Yang Xihua , Lv Caixia , Wang Zhixiang , Hu Linjie , Li Zheng , Ma Jinfeng , Zhang Huanhu , Lu Xiaoqing 
Published: March 2023

IntroductionA number of evidences have proved that “Nostoc commune” Vauch can improve human immunity and prevent diseases, however, the specific mechanism remains unclear. The biological activity of the main protein component of “Nostoc commune” Vauch extracellular matrix– a water-stress protein (WSP) still needs to be elucidated.MethodsIn our study, we validated the role of WSP in gastric cancer metastasis at the cellular level, the organoid level and in mouse models, and also studied the role of EGFRVIII and downstream signaling molecules after WSP treatment.ResultsWe found that WSP can significantly inhibit the metastasis of gastric cancer cells. Interestingly, we found that the anti-metastasis ability of WSP on gastric cancer was related to membrane protein receptor EGFRVIII, which was realized by inhibiting the downstream EGFRVIII signaling pathway. In terms of mechanism, WSP can inhibit the downstream EGFRVIII signaling pathway Akt-PI3K and further inhibit the secretion of cancer-related metastasis proteins such as MMP2 and MMP9, thus, significantly affecting the metastasis of gastric cancer cells.DiscussionGiven the anticancer properties of WSP, drug developers and manufacturers can further develop protein drugs for cancer patients using protein engineering techniques based on the properties of WSP.