bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Evaluation of Metamorphopsia Score Before and After Pars Plana Vitrectomy Operation In Patients with Idiopatic Epiretinal Membranes Using The M-Charts™ and Comparison with Oct Parameters
Authors: OCAL Olgar , DOGAN Mehmet Erkan , BILGIN Ahmet Burak 
Published: August 2023

Abstract
Purpose: It was aimed to quantitatively evaluate metamorphopsia pre- and postoperatively in patients with idiopathic epiretinal membrane(iERM) using the M-CHARTS™ and to evaluate the relationship between changes in retinal layers in optical coherence tomography (OCT) and metamorphopsia scores.
Materials and Methods:This prospective study included patients followed up in Akdeniz University Hospital Ophthalmology Clinic diagnosed with iERM by fundus examination and optical coherence tomography between 2020-2022. Detailed ophthalmologic findings, visual acuities and metamorphopsia scores and OCT parameters of all patients were recorded.The relationship between pre- and postoperative visual acuity, metamorphopsia scores and OCT parameters was investigated. The changes of oct parameters were compared by evaluating patients with decreased metamorphopsia score as one group and patients with stable or increasing metamorphopsia score as the other group.
Results: In the group with decreased metamorphopsia score, pre-treatment and post-treatment 12th month GCL+IPL (C) (Ganglion Cell Layer+Inner Plexiform Layer (Central)) value was significantly (p<0.05) higher than the group with metamorphopsia score increase.
Conclusion:Statistically significant preoperative thickness difference in the OCT parameters of the GCL+IPL (C) layer in the group with decrease and increase in metamorphopsia scores was associated with the quantitative metamorphopsia complaints of the patients. The thickness of the preoperative GCL+IPL (C) layer can be considered as an important parameter for the symptoms of metamorphopsia that determines the functional success after the surgery.
Diversity of Moths (Insecta: Lepidoptera) Supported by Sandalwood (Santalum Album L.) in South India
Authors: Padma S , Kavya N , Manjula K N , Shanbhag R. R. , Sundararaj R 
Published: August 2023

Abstract
The evergreen and perennial Royal tree, Santalum album L., is an eco-conservator because it houses a wide variety of organisms. One such group is moths. Intensive surveys were conducted in sandalwood plantations in south India to understand the diversity of moths supported by sandalwood. A total of 56 species of moths belonging to 15 families were found in sandalwood, and the record of 27 moths forms the first report on sandalwood. Out of 15 families, the family Erebidae is dominant with twenty-one species, followed by Geometridae with eight species and Psychidae with six species each. The remaining moths include three species each of Cossidae and Limacodidae; two species each of Crambidae, Eupterotidae, Lecithoceridae, Noctuidae and Tortricidae; and a single species in each of the remaining 5 families. Further, the pruned plantations showed a significantly negative impact on the diversity of moths and greatly influenced the level of their infestation on the trees. Implications for insect conservation: Our analysis provides an effective groundwork for addressing the principle of conservation for not only target insect species but also in general. Further, immediate action is specified for the protection of vegetation and proper maintenance of structural integrity of trees, which directly influences the distribution and habitats of insects.
Two-repeat units of CG-rich trinucleotides form long-sought hotspot loci shared across primates and mouse
Authors: Ohadi Mina , Tajeddin Nahid , Bayat Hadi , Arabfard Masoud , Maddi Ali M. A. , Moghadam Masoumeh Goleyjani , Khamse Safoura , Alizadeh Samira , Delbari Ahmad , Khorshid Hamid Reza Khorram 
Published: August 2023

Abstract
Background
CG-rich trinucleotide short tandem repeats link with human cognition and various neurodevelopmental, neurological, and movement disorders. However, the fundamental two-repeat units of these STRs are literally unexplored.
Results
On a genome-wide scale, here we mapped the two-repeat units of all combinations of trinucleotides in human, and found thousands of colonies (distance between each unit < 500 bp) of exceedingly significant occurrence based on Poisson distribution. Subsequently, we performed a comparative genomics study of several of the large and medium-size colonies in other primates and mouse. We discovered that some of those colonies were shared with extensive dynamicity, as phylogenetically distant as in mouse. We found evidence of emergence and propagation of several non-coding RNAs, such as PIWI-interacting RNAs (piRNAs) and uncharacterized RNAs in some colonies.
Conclusion
We report evidence of extensively dynamic trans-species crossing-over and conversion events in the two-repeat unit colonies of CG-rich trinucleotides, which among many evolutionary implications, may be a novel mechanism for the emergence and propagation of non-coding RNAs in some colonies. This is the first evidence of loci of massive genomic hotspot events, shared at least across primates and mouse.
Identification of Active Components and Antifungal Activities of Extracts from Carpesium abrotanoides L. (Asteraceae)
Authors: Zhu Hongxia , Hu Linfeng , Gao Ge , Wang Yunlong 
Published: August 2023

Abstract
The target-directed efficient extraction and antifungal activities of extracts against seven phytopathogenic fungi from flowers, leaves and seeds of Carpesium abrotanoides L. (Asteraceae) were evaluated. In the seven tested fungi, the extracts of three parts of this plant produced by petroleum ether and chloroform, as well as extracts of flower and seed produced by ethyl acetate, had strong activities against Gibberella zeae, Phytophthora capsica and Rhizoctonia cerealis. The seed extract produced by chloroform was further fractionated to seven fractions [SS2(1–7)] using bioautography-guided TLC and its high active fraction (SS4) was identified using ultra-performance liquid chromatography–mass spectrometry. Eight compounds with higher contents in SS2-4 were identified. Among them, dehydrocostus lactone, isoalantolactone, parthenolide and alantolactone showed different concentration-dependent antifungal effects on R. cerealis, resulting in EC50s of 7.05, 18.22, 21.22 and 125.10 mg/L, respectively.
Training and brain stimulation in patients with cognitive impairment: a randomized controlled trial
Authors: Antonenko Daria , Fromm Anna Elisabeth , Thams Friederike , Kuzmina Anna , Backhaus Malte , Knochenhauer Elena , Li Shu-Chen , Grittner Ulrike , Flöel Agnes 
Published: August 2023

Abstract
Background
Repeated sessions of training and non-invasive brain stimulation have the potential to enhance cognition in patients with cognitive impairment. We hypothesized that combining cognitive training with anodal transcranial direct current stimulation (tDCS) will lead to performance improvement in the trained task and yield transfer to non-trained tasks.
Methods
In our randomized, sham-controlled, double-blind study, 46 patients with cognitive impairment (60–80 years) were randomly assigned to one of two interventional groups. We administered a 9-session cognitive training (consisting of a letter updating and a Markov decision-making task) over 3 weeks with concurrent 1-mA anodal tDCS over left dorsolateral prefrontal cortex (20 min in tDCS, 30 sec in sham group). Primary outcome was trained task performance (letter updating task) immediately after training. Secondary outcomes included performance in tasks testing working memory (N-back task), decision-making (Wiener Matrices test) and verbal memory (verbal learning and memory test), and resting-state functional connectivity (FC). Tasks were administered at baseline, at post-assessment, and at 1- and 7-month follow-ups (FU). MRI was conducted at baseline and 7-month FU. Thirty-nine participants (85%) successfully completed the intervention. Data analyses are reported on the intention-to-treat (ITT) and the per-protocol (PP) sample.
Results
For the primary outcome, no difference was observed in ITT or PP sample. However, the tDCS outperformed the sham group in the N-back working memory task. Frontoparietal network FC was increased from baseline to 7-month FU in the tDCS compared to the sham group. Exploratory analyses showed a correlation between individual memory improvements and higher electric field magnitudes induced by tDCS. Adverse events did not differ between groups, questionnaires indicated successful blinding.
Conclusions
In sum, cognitive training with concurrent brain stimulation induced transferred working memory benefits in patients with cognitive impairment who underwent the full 3-week intervention. MRI data pointed toward a potential intervention-induced modulation of neural network dynamics. A link between individual performance gains and electric fields suggested dosage-dependent effects of brain stimulation. Together, our findings show that the combined intervention might be a potential treatment option in patients with cognitive impairment. Future research needs to explore whether individualized protocols for both training and stimulation parameters might further enhance treatment gains.
Trial registration
The study is registered on ClinicalTrials.gov (NCT04265378). Registered on 7 February 2020. Retrospectively registered.
A comparative study of machine learning methods for assessing the compressive strength of Pozzolanic concrete
Authors: Raju Matiur Rahman , Rahman Mahfuzur , Hasan Md Mehedi , Sharmily Tarin , Hosen Mohammed Sajib , Islam Md Monirul , H Noor Md. Sadiqul 
Published: August 2023

Abstract
This study aims to enhance the prediction of compressive strength in pozzolanic concrete by leveraging machine learning techniques. Unlike conventional methods relying on costly lab tests or empirical correlations, machine learning offers a more precise and efficient approach. Three models—artificial neural networks (ANNs), random forest (RF), and gradient boosting regressor (GBR)—are harnessed to develop predictive models. The dataset, comprising 482 samples, is divided randomly into 70% (337 samples) for training and 30% (145 samples) for testing. Seven input parameters related to pozzolanic material type, proportion, and mix design are utilized for model training. Model performance assessment employs metrics such as coefficient of determination (R2), root mean square error (RMSE), and mean absolute error (MAE). Notably, the RF model outperforms others, achieving the highest R2 of 0.976 in training and 0.964 in testing, with the lowest RMSE (2.84) and MAE (2.05) in training and 7.81 and 5.89, respectively in testing, demonstrating superior predictive precision. The model’s accuracy is evaluated using the Taylor diagram. Additionally, sensitivity analysis reveals cement as the most impactful input parameter, influencing 28% of variability. The RF model’s robustness is confirmed through K-fold cross-validation, yielding an average R2 of 0.959. This study underscores the reliability and effectiveness of the RF model for forecasting pozzolanic concrete’s compressive strength, carrying implications for optimizing concrete mix and construction practices. Overall, the proposed RF model excels in efficiency and accuracy, establishing its supremacy over other algorithms in predicting pozzolanic concrete properties.
MiR-130a-3p plays a key role in adipocyte differentiation of orbital fibroblasts (OFs) in Graves' ophthalmopathy by regulating the Wnt/β-catenin signaling pathway
Authors: YE Xiaozhen , wang gengwang , Liu Jun , LU Bin , SHAO Jiaqing 
Published: August 2023

Abstract
Objective
To study the effects of miR-130a-3p on the adipocyte differentiation of orbital fibroblasts in Graves’ ophthalmopathy (GO).
Methods
The expression level of key transcription factors in the Wnt/β-catenin signaling pathway and adipocytes were detected in the human primary orbital fibroblasts (OFs) obtained from GO, non-GO patients or OFs (GO) treated with LiCl (Wnt/beta-catenin pathway activator). The content of IL-1β, TNF-α and IFN-γ and the expression level of ICAM-1, COX-2, and MCP-1 were detected in OFs (GO) or OFs (GO) treated with LiCl. The effects of miR-130a-3p mimics on the differentiation of orbital fibroblasts and the potential mechanisms were investigated.
Results
The ratio between the expression level of p-GKS-3β、GKS-3β and β-catenin was decreased significantly, the PPAR-γ and C/EBPα were increased significantly in OFs (GO). After the treatment of LiCl in OFs (GO), the ratio between the expression level of p-GKS-3β、GKS-3β and β-catenin were increased, while PPAR-γ and C/EBPα were decreased. Cell viability, the number of adipocytes, IL-1β, TNF-α, IFN-γ, ICAM, COX-2, and MCP-1were decreased significantly in OFs (GO) treated with LiCl. The expression of related proteins was reversed after OFs (GO) treated with miR-130a-3p mimics, the alterations induced by miR-130a-3p mimics were reversed by pcDNA- PPAR-γ.
Conclusions
In the present study, miR-130a-3p inhibited adipocyte differentiation in OFs from patients with GO. The underlying mechanism might be the negative regulation of PPARγ-mediated adipogenesis via the Wnt/β-catenin signaling pathway by miR-130a-3p. MiR-130a-3p might be an useful target in treating Graves' ophthalmopathy.
Biosynthesis of lead oxide nanoparticles using Ocimum lamiifolium leaves extract
Authors: Habtemariam Asratemedhin Bekele 
Published: August 2023

Abstract
In this study we report the biosynthesis of lead oxide nanoparticles from Ocimum Lamiifolium aqueous extract and lead citrate precursor. Characterization techniques such as powder X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and UV-Visible spectroscopy were used to study the desired phase formation, crystal structure and morphology of the synthesized nanostructure. The powder X-ray diffraction analysis revealed that lead oxide nanoparticles with an average crystallite size of 39 nm have been synthesized. Scanning electron microscopy analysis exhibited the surface morphology. Fourier transform infrared spectroscopy study also confirmed also the successful synthesis of lead oxide nanoparticles. The UV-Visible spectrum absorbance peak is in the range of 200–250 nm and this corresponds to the characteristic of lead oxide nanoparticles.
Designing Pyrrolopyrimidine Linked Triazole Derivatives as JAK Inhibitors for Anti- Cancer Activity: Molecular Docking Analysis and ADMET Prediction
Authors: Gaikwad Shraddha P , Jadhav Shailaja B , Chaudhari Somdutta 
Published: August 2023

Abstract
Janus kinases (JAKs) are a family of non-receptor tyrosine kinases that play a central role in the signaling pathways of many cellular processes, including inflammation, cell growth, and differentiation. JAK inhibitors have been shown to have potential as anticancer agents. In this study, a series of pyrrolopyrimidine-linked triazole derivatives were designed and synthesized. The molecular docking studies were performed using Auto Dock Vina to evaluate the binding affinity of the compounds with JAK. The ADMET properties of the compounds were predicted using the ADMET Predictor software. The molecular docking studies showed that the compounds have a good binding affinity with JAK. The ADMET properties of the compounds were predicted to be favorable. According to a study on Passonline study, the substances will react with thioredoxin inhibitor activity with the fewest negative effects. Given that the proposed compounds only violate one of the "rules of five," it can be said that these derivatives may have oral activity. All of the proposed compounds can be regarded as safer lead molecules based on the ADMET study. based on the docking Analysis revealed that the derivatives had the lowest minimum binding energy to the protein and had a strong binding affinity. The docking results indicated that the compounds D2 and D6 respectively, would be the most potent inhibitors. Hydrophobic and hydrogen bond interactions occur between the molecules. These results suggest that the pyrrolopyrimidine-linked triazole derivatives have the potential as JAK inhibitors for anticancer activity.
Global trends, knowledge mapping and visualization of current research on climate change and their impact on plant-pollinators interaction
Authors: Singh Pooja , Gargi Baby , Semwal Prabhakar 
Published: August 2023

Abstract
The mutualistic interactions between plants and pollinators is one of the most prestigious field of research in ecology, which give information on the biotic network architecture, coexistence, diversification, and ecosystem function. In this manuscript, our research intends to identify the published literature for research production, collaborations, hotspots, and trends in research addressing the influence of climate change on the relationship between plants and pollinators. The research and review papers on the interactions between plants and pollinators, and climate change published in the Scopus database were retrieved. The contribution of nations, journals, institutions, current trends, and keyword analysis were shown using VOSviewer and RStudio bibliometrix to produce a network map of author collaboration across nations. The Scopus database (2007 to 2023) yielded a total of 256 entries, of which 229 publications were examined after being excluded based on various criteria. As per our data analysis, “The Rocky Mountain Biological Laboratory” and “Journal of Ecology” were the most effective organization, and journal, respectively. The author with the most publications was “Laura A. Burkle” of “Montana State University”. The “United States” is rated #1 among all nations in terms of the global production of literature on the effects of climate change on interactions between plant and pollinators. We determined four primary research subjects and new research areas for further study through the analysis of keywords. This study highlights current research on the effects of climate change on plant-pollinator relationships and is an early attempt to understand how these connections form and alter. The majority of recent research has been on the effects of drought on plants in these systems, which is a developing field of study that requires attention from academics for future research.
Direct specimen collection during routine operation improves nucleic acid quality in genetic analysis samples for head and neck tumors: a retrospective study
Authors: Noda Yuri , Sakagami Tomofumi , Suzuki Kensuke , Fujisawa Takuo , Yagi Masao , Iwai Hiroshi , Tsuta Koji 
Published: August 2023

Abstract
Background
The success of sequencing analysis and genetic treatment is influenced by sample quality. Preserving nucleic acid integrity in head and neck squamous cell carcinoma (HNSCC) samples is challenging owing to poor formalin fixation caused by lengthy surgical procedures and demineralization. New sampling and fixation methods are required to prevent the loss of important variants. We aimed to improve nucleic acid preservation in HNSCC specimens using a new collection method.
Methods
A total of 128 samples from 44 patients with HNSCC were included: 32 genetic analysis samples (GAS), which were collected from the tumor surface in the operation room and immediately placed in a 25 mL bottle with 10% neutral buffered formalin solution; 43 primary tumor components (Primary); 14 decalcified tumor samples (DC); 32 metastatic tumors in lymph nodes (LN); and seven parakeratinized components (PKC) from HNSCC. The quality of nucleic acids in the GAS, Primary, DC, LN, and PKC samples was compared using the DNA integrity number (DIN), RNA integrity number (RIN), the percentage of RNA fragments with > 200 nucleotides (DV200), and methyl green-pyronin (MGP) staining. The next-generation sequencing (NGS) metrics of the Primary, LN, and PKC from three HNSCC samples were also assessed.
Results
The DIN was significantly higher in the GAS than in the Primary, LN, and DC groups (p < 0.05). The RIN decreased in the order LN, GAS, Primary, and DC. DV200 was significantly higher in the GAS than in the primary and DC groups (p < 0.05). On MGP staining, the preserved DNA and RNA were visualized in the GAS, Primary tumors from 2022, and LN, but not in DC. The PKC samples contained DNA, but RNA was not detected. NGS, using DNA extracted from the PKC samples, reliably detected mutations. No significant differences were detected in most NGS metrics among the Primary, LN, and PKC samples.
Conclusions
GAS should be collected during routine hospital activities, especially for head and neck tumors. Moreover, when the volume of viable materials is limited, PKC should be considered for genetic analysis. Finally, MGP staining is useful for pre-pathological analysis.
Unleashing Economic Potential: Decoding the Fdi-economic Growth Nexus in G-15 Economies Amidst Unique Host Country Traits
Authors: BAJAJ AASTHA ,   2.	Dr Lakshmi Bhooshetty
Published: August 2023

Abstract

The study aims to examine the impact of Foreign Direct Investment (FDI) on Economic Growth in G-15 countries while considering the influence of key host country characteristics, namely Macroeconomic Stability, Financial Development, Human Capital, and Trade Openness. The selection of these variables is supported by both theoretical foundations and empirical studies that highlight their significant role in shaping the FDI-growth interconnection. Panel data derived from World Bank Indicators, spanning the period from 1989 to 2021 are analysed using a Feasible Generalized Least Squares method (FGLS). The findings demonstrated that increased Financial Development, Human Capital, and Trade Openness enhanced the positive effects of FDI on economic growth. However, Inflation has a negative effect on growth. Policymakers in these nations should give precedence to developing strong financial markets, promoting trade liberalization, and investing in human capital to optimize the advantages of FDI. This research addresses a critical gap in the literature as limited empirical work has been conducted on the FDI-growth relationship specific to the G-15 economies. By employing rigorous panel data methodology and a long-term dataset, this study provides original insights into the interaction between FDI and host country characteristics, contributing to the existing body of knowledge.