bluuebunny/crossref_metadata_2025_split huggingface dataset:
Niclosamide potentiates TMEM16A and induces vasoconstriction
Authors: Liang Pengfei , Wan Yui Chun S. , Yu Kuai , Hartzell H. Criss , Yang Huanghe 
Published: August 2023

ABSTRACTThe TMEM16A calcium-activated chloride channel is a promising therapeutic target for various diseases. Niclosamide, an anthelmintic medication, has been considered as a TMEM16A inhibitor for treating asthma and chronic obstructive pulmonary disease, but was recently found to possess broad-spectrum off-target effects. Here we show that, under physiological conditions, niclosamide acutely potentiates TMEM16A without having any inhibitory effect. Our computational and functional characterizations pinpoint a putative niclosamide binding site on the extracellular side of TMEM16A. Mutations in this site attenuate the potentiation. Moreover, niclosamide potentiates endogenous TMEM16A in vascular smooth muscle cells, triggers intracellular calcium increase, and constricts the murine mesenteric artery. Our findings advise caution when considering niclosamide as a TMEM16A inhibitor to treat diseases such as asthma, COPD, and hypertension. The identification of the putative niclosamide binding site provides insights into the mechanism of TMEM16A pharmacological modulation, shining light on developing specific TMEM16A modulators to treat human diseases.
A demand-based framework explains prioritization strategies upon transient limitations of different amino acids
Authors: Gupta Ritu , Adhikary Swagata , Dalpatraj Nidhi , Laxman Sunil 
Published: August 2023

AbstractCells require disparate amounts of distinct amino acids, which themselves have discrete biosynthetic costs. However, it remains unclear if and how cells respond differently to their scarcity. To explore this, we re-organized amino acids into distinct groups based on their metabolic origins. Subsequently, using yeast we assessed responses to transient disruptions in amino acid supply, and uncover diverse restoration responses for distinct amino acids. Cells hierarchically prioritize restoring glutamate-, sulfur-, pentose-phosphate- and pyruvate-derived amino acids. Particularly, the strongest response is to the glutamate-derived amino acid arginine. We find that the extent and priority of the restoration response is determined by the composite demand for an amino acid, coupled with low individual biosynthetic costs of that amino acid. We propose that cells employ a conserved strategy guided by the law of demand, to prioritize amino acids restoration upon transient limitation.
Chondroitin and dermatan sulfate exposure induces a wound healing state in fibroblasts through Cux1-mediated SerpinB2 transcriptional repression
Authors: Diaz-Pizarro Alba , Del Valle-Del Pino Nuria , Galán Enrique , Carvajal-González Jose María , Román Ángel-Carlos , Mulero-Navarro Sonia 
Published: August 2023

ABSTRACTMucopolysaccharidoses (MPS) are a group of syndromes characterized by the accumulation of sulfated glycosaminoglycans (sGAGs), leading to profound connective tissue alterations, including impaired endochondral ossification. The function of sGAGs involves determining the mechanical properties of the extracellular matrix and regulating growth factor signaling pathways, such as Fgf2. In this study, we investigated the deposition of chondroitin sulfate and dermatan sulfate, two major sGAGs, and their resemblance to wound healing states in human fibroblasts. Our findings indicate that this condition alters cell adhesion, providing a potential explanation for fibrosis-like changes observed in MPS patients. Furthermore, we elucidate the molecular pathway responsible for this effect, wherein increased Cathepsin L activation leads to the processing of the transcription factor Cux1 into a stable form capable of regulating the expression of target genes, including SERPINB2. The presence of similar changes in cell adhesion in human-induced pluripotent stem cell-derived mesenchymal cells further reinforces the significance of sGAGs in cell adhesion and sheds light on possible mechanisms underlying altered endochondral ossification in MPS patients.
Recognition of Necrosis and Ethylene-inducing like peptides confers disease resistance inBrassica napusand is modulated byBSK1in Arabidopsis
Authors: Yalcin Hicret Asli , Ramirez-Gonzalez Ricardo Humberto , Jacott Catherine N. , Steuernagel Burkhard , Sidhu Gurpinder Singh , Kirby Rachel , Verbeek Emma , Schoonbeek Henk-jan , Ridout Christopher J. , Wells Rachel 
Published: August 2023

AbstractBrassicas are important crops susceptible to significant losses caused by disease: thus, breeding resistant lines can mitigate the effects of pathogens. MAMPs (microbe-associated molecular patterns) are conserved molecules of pathogens that elicit host defence responses known as pattern-triggered immunity (PTI). Necrosis & Ethylene-inducing peptide 1-like proteins (NLPs) are MAMPs found in a wide range of phytopathogens, including major disease-causing fungal species. We studied the response to the BcNEP2 fromBotrytis cinereaas a representative NLP inBrassica napusto improve our understanding of recognition mechanisms that could enable the development of disease-resistant crops.To genetically map regions responsible for NLP recognition, we used an associative transcriptomics (AT) approach using diverseB. napusaccessions and bulk segregant analysis (BSA) on DNA pools created from a bi-parental cross of NLP-responsive (Ningyou1) and non-responsive (Ningyou7) lines. In silicomapping with AT identified two peaks associated with NLP recognition on chromosomes A04 and C05 whereas the BSA narrowed it down to a main peak on A04. BSA delimited the region associated with NLP-responsiveness to 3 Mbp, containing ∼245 genes on the Darmor-bzhreference genome. Variants detected in the region were used for KASP marker design and four KASP markers were identified co-segregating with the phenotype. The same pipeline was performed with the ZS11 genome, and the highest associated region was confirmed on chromosome A04. Comparative BLAST analysis revealed there were unannotated clusters of RLP homologs on ZS11 chromosome A04. To reduce the number of candidate genes responsible for NLP recognition, RNA-Seq data was used to detect the unannotated expressed putative genes. Screening the BSA Ning1×7 population demonstrated a highly significant association between NLP-recognition and resistance toBotrytis cinerea. Also, the lines non-responsive to NLP had significantly greater response to the bacterial MAMP flg22. Additionally,BnaA01g02190D, a homologue of ArabidopsisAtBSK1(At4g35230)BR-SIGNALLING KINASE1,was associated with a high BcNEP2-induced ROS response phenotype. We show that in Arabidopsis,Atbsk1mutants had significantly lower response to BcNEP2 and increased susceptibility toB. cinerea(p-value=1.12e-14***). Overall, the results define the genomic location for NLP-recognition on theB. napusgenome and demonstrate that NLP recognition has a positive contribution to disease resistance which can have practical application in crop improvement.
Chaperone-assisted cryo-EM structure ofP. aeruginosaPhuR reveals molecular basis for heme binding
Authors: Knejski Paweł P. , Erramilli Satchal K. , Kossiakoff Anthony A. 
Published: August 2023

SUMMARYPathogenic bacteria, such asPseudomonas aeruginosa, depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein inP. aeruginosaclinical isolates. However, a comprehensive understanding of heme recognition and TBDT transport mechanisms, especially PhuR, remains limited. In this study, we employed single-particle cryogenic electron microscopy (cryo-EM) and a phage display-generated synthetic antibody (sAB) as a fiducial marker to enable the determination of a high-resolution (2.5 Å) structure of PhuR with a bound heme. Notably, the structure reveals iron coordination by Y529 on a conserved extracellular loop, shedding light on the role of tyrosine in heme binding. Biochemical assays and negative-stain EM demonstrated that the sAB specifically targets the heme-bound state of PhuR. These findings provide insights into PhuR’s heme binding and offer a template for developing conformation-specific sABs against outer membrane proteins (OMPs) for structure-function investigations.
Helping Dwight: how gamification can improve CSR communication effectiveness?
Authors: Wójcik Filip 
Published: August 2023

PurposeThe main purpose of this study is to check the potential impact of gamification on communicating CSR issues.Design/methodology/approachThe examination was conducted concerning the correlations between income/education level and communication effectiveness with and without gamification application. For the need of this study the survey was prepared, containing inter alia narration resting on helping Dwight to deal with the problems with adjusting to work environment, the problem often avoided in corporate social responsibility (CSR) communication research, especially gamified.FindingsFindings show that gamification can help in this area, but depending on the author’s goal (spreading the news is the most relevant one).Originality/valueAlthough the gamification gained substantial interest over the last decade, the results of applying the same into CSR communication are still very rare.
BFFNet: a bidirectional feature fusion network for semantic segmentation of remote sensing objects
Authors: Hou Yandong , Wu Zhengbo , Ren Xinghua , Liu Kaiwen , Chen Zhengquan 
Published: August 2023

PurposeHigh-resolution remote sensing images possess a wealth of semantic information. However, these images often contain objects of different sizes and distributions, which make the semantic segmentation task challenging. In this paper, a bidirectional feature fusion network (BFFNet) is designed to address this challenge, which aims at increasing the accurate recognition of surface objects in order to effectively classify special features.Design/methodology/approachThere are two main crucial elements in BFFNet. Firstly, the mean-weighted module (MWM) is used to obtain the key features in the main network. Secondly, the proposed polarization enhanced branch network performs feature extraction simultaneously with the main network to obtain different feature information. The authors then fuse these two features in both directions while applying a cross-entropy loss function to monitor the network training process. Finally, BFFNet is validated on two publicly available datasets, Potsdam and Vaihingen.FindingsIn this paper, a quantitative analysis method is used to illustrate that the proposed network achieves superior performance of 2–6%, respectively, compared to other mainstream segmentation networks from experimental results on two datasets. Complete ablation experiments are also conducted to demonstrate the effectiveness of the elements in the network. In summary, BFFNet has proven to be effective in achieving accurate identification of small objects and in reducing the effect of shadows on the segmentation process.Originality/valueThe originality of the paper is the proposal of a BFFNet based on multi-scale and multi-attention strategies to improve the ability to accurately segment high-resolution and complex remote sensing images, especially for small objects and shadow-obscured objects.
From mental pictures to mental movies: a new approach to positioning of innovations
Authors: Sääksjärvi Maria 
Published: August 2023

Purpose
As innovations introduce novel benefits to customers, they would need to be positioned in a way that sets them apart in the market. The purpose of this paper is to propose a novel approach for the positioning of innovations with the use of the customer imagination and, specifically, mental movies.

Design/methodology/approach
Using the schema approach as this study’s theoretical framework, the author proposes that innovations could be positioned using moving pictures (i.e. mental movies) instead of mental pictures (the approach traditionally taken).

Findings
A new conceptual framework for the positioning of innovations using mental movies is presented. In the framework, this study outlines how innovations can be positioned with the use of mental movies, and why such an approach would be beneficial. The framework outlines mixed reality, i.e. augmented reality, augmented virtuality and virtuality, as well as the metaverse and gaming as avenues for positioning innovations using mental movies. On the benefit side, the framework identifies successful market introductions, engagement and stickiness, memorability and positive emotions, uniqueness and differentiation and market share as the concrete benefits that can be achieved with this type of positioning.

Originality/value
The framework provides a novel approach for the positioning of innovations. It departs from existing literature by proposing that innovations can be positioned using mental movies. The framework also identifies why this approach would be beneficial for marketers and managers and provides concrete guidelines for how such a positioning can be achieved in the market.
High-performance work systems and employee attitudes: evidence from Indian healthcare industry
Authors: Padamata Karthik , Vangapandu Rama Devi 
Published: August 2023

PurposeBy following the “employee-centric” approach, this study aims at identifying the impact of high-performance work systems (HPWS) on specific employee attitudinal outcomes such as work engagement, job satisfaction and affective commitment in the Indian healthcare industry.Design/methodology/approachThe target population for this study includes the nurses working in large private multi-specialty tertiary care hospitals in India. Partial Least Squares Structural Equation Modelling (PLS-SEM) techniques are used on a sample of 152 nurses working in two large specialty hospitals.FindingsIn the Indian healthcare industry context, the nurse's perception of HPWS has shown a significant positive effect on their attitudinal variables such as work engagement, job satisfaction and affective commitment. When checked for mediation of work engagement and job satisfaction variables in HPWS – affective commitment relationship, nurse's job satisfaction partially mediated the relationship, but nurse's work engagement has shown no mediation effect.Originality/valueThis is one of the pioneering studies conducted in the Indian healthcare industry context, especially on the nurse's sample in identifying the impact of high-performance work systems on their attitudinal outcomes. Underscoring the paucity of HPWS research in the Indian healthcare industry, this study's findings will be an addition to the HPWS literature and also to the nursing research in the Indian healthcare settings.
COVID-19 and disruptive technology in New Zealand
Authors: Mat Aripin Asma , Brougham David 
Published: August 2023

PurposeCOVID-19 has immensely disrupted business dynamism, providing catalyst innovation opportunities and transposing society's perception of disruptive technology (DT). This research increases the understanding of the impact of the pandemic in influencing the way organizations perceive DT and whether any mitigating factors were considered when deciding to adopt new technology during the pandemic.Design/methodology/approachA qualitative approach was adopted in this research, consisting of 14 semi-structured interviews with eight senior managers and six employees, representing both the private and public sectors in New Zealand. All participants had in-depth knowledge of organizational DT adoption during the pandemic. Two separate sets of semi-structured interviews were used to enable comparison between senior managers' and employees' experiences of organizational adoption of DT post-emergence of COVID-19. Due to the nature of this research being conducted on organizational adoption of DT during the pandemic, time constraints and sample size were two of the key limitations of this research. Specifically, potential participants widely cited unavailability due to additional pressure from COVID-19. Given the limited research in this area, this study is explorative by nature and adds significant insights to the literature.FindingsThe findings suggest that COVID-19 has contributed towards an increased acceptance of, reliance on and adoption of DT across both organizational and social landscapes. The authors found that one of the reasons COVID-19 expedites the adoption of DT correlates with the notion of technology dependency, with organizations citing DT as a viable part of a business continuity plan (BCP) to counter the unpredictability of ongoing disruptive events associated with COVID-19 or any similar disruption which may be on the horizon. These findings are highly relevant as they suggest that the labor market in New Zealand is flexible so organizations and employees can adapt to DT and COVID-19.Originality/valueThis research adds much-needed insight into the emerging field of research that examines COVID-19's impact on the adoption of DT from both management and employee perspectives.
Antecedents of using the online food delivery subscription services
Authors: Sorathia Nefike Gunden , Morosan Cristian 
Published: August 2023

Purpose
This study aims to explicate consumers’ intentions to use online food delivery systems (OFDS) subscription services. The study revisited the unified theory of acceptance and use of technology.

Design/methodology/approach
A typical methodology that involved a confirmatory factor analysis, followed by structural equation modeling, was used to test the hypotheses using a sample of 573 OFDS users from the USA.

Findings
The results revealed that social influence has the most significant impact on consumers’ intentions to use OFDS subscription services, while effort expectancy and perceived security have relatively lower impacts on consumers’ intentions to use OFDS subscription services. In addition, the study revealed the role of compatibility and convenience orientation in shaping consumers’ OFDS system perceptions (e.g. performance and effort expectancy).

Research limitations/implications
The results provide several theoretical and managerial implications and open new avenues for future research. The study advances the literature by validating the set of antecedents of important core perceptions (e.g. performance expectancy and effort expectancy). The study also provides implications for two types of practitioners, such as OFDS and restaurants.

Originality/value
To the best of the authors’ knowledge, this is the first study to examine consumers’ intentions to use OFDS subscription services. This study is also the first to examine key factors influencing consumers’ intentions to use OFDS subscription services.
Happiness and its relationship to expectations of change and sustainable behavior in a post-COVID-19 world
Authors: Contreras-Contreras Pablo , Cuesta-Valiño Pedro , Gutiérrez-Rodríguez Pablo 
Published: August 2023

PurposeThis study aims to analyze the relationship between expectations of change after a crisis, such as that generated by COVID-19, people's levels of happiness and the propensity for pro-sustainable behavior.Design/methodology/approachSurveys were conducted using 1,130 observations. The aim was to measure expectations of change during the pandemic. Furthermore, the relationship between these expectations, happiness levels, and attitudes towards sustainability was analyzed for various elements of daily life. Statistical techniques such as principal component analysis (PCA), cluster analysis, variance analysis and correlation analysis were utilized to explore the underlying patterns and relationships.FindingsOptimistic expectations for post-pandemic change are associated with a greater likelihood of engaging in environmentally beneficial behaviors, leading to higher levels of life satisfaction. This correlation is particularly significant when individuals envision broader transformations in the collective behavior of humanity. These findings suggest a strong link between beliefs in positive societal transformations and both pro-environmental actions and personal well-being.Research limitations/implicationsThe unique circumstances of the pandemic, characterized by heightened media consumption, likely influenced the expectations of individuals, particularly fostering pessimistic outlooks in critical situations. Clear and solid variables were utilized despite not employing validated scales to measure expectations. However, it has been proven that there is a clear link between change expectations, happiness, and the propensity for a more sustainable daily life.Practical implicationsThis study identifies guidelines that strengthen brands' communication strategies based on individuals' sustainability profiles and visions of the future.Social implicationsThere is a need to target skeptical, change-resistant segments of the population with a more convincing and solid discourse to promote sustainable consumption and behavior.Originality/valueThis is the first study to simultaneously address the relationship between individuals' expectations of change following traumatic events such as the pandemic, their engagement in sustainable behavior, and their increased levels of happiness.
Government expenditure impacts on financial development: Do population age structures moderations matter?
Authors: Kapaya Saganga Mussa 
Published: August 2023

PurposeThis study examined the roles of public spending and population moderating characteristic structure of selected African economies on bank-based financial development through credit to private sector.Design/methodology/approachThe study sampled 37 selected African economies for the years 1991–2018, and it applied a pooled mean group (PMG) estimator to account for short-run and long-run causal effects, and confirmed short-run adjustments towards the long-run convergences between the variables. Specific suitable tests were also applied.FindingsEvidence confirms positive impacts of both capital formation and final consumption expenditures on financial development in the short run and long run. The moderation of population structures on expenditure structures help to speed up convergences.Originality/valueThis work attests its innovation by accounting for the separate effects of the expenditure types, the moderation effects of young and mature populations for capital and final consumption expenditure on financial development among selected economies in Africa.