bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of function of GATA3 regulates FRA1 and c-FOS to activate EMT and promote mammary tumorigenesis and metastasis
Authors: Liu Xiong , Bai Feng , Wang Yuchan , Wang Chuying , Chan Ho Lam , Zheng Chenglong , Fang Jian , Zhu Wei-Guo , Pei Xin-Hai 
Published: June 2023

AbstractBasal-like breast cancers (BLBCs) are among the most aggressive cancers, partly due to their enrichment of cancer stem cells (CSCs). Breast CSCs can be generated from luminal-type cancer cells via epithelial-mesenchymal transition (EMT). GATA3 maintains luminal cell fate, and its expression is lost or reduced in BLBCs. However, deletion of Gata3 in mice or cells results in early lethality or proliferative defects. It is unknown how loss-of-function of GATA3 regulates EMT and CSCs in breast cancer. We report here that haploid loss of Gata3 in mice lacking p18Ink4c, a cell cycle inhibitor, up-regulates Fra1, an AP-1 family protein that promotes mesenchymal traits, and downregulates c-Fos, another AP-1 family protein that maintains epithelial fate, leading to activation of EMT and promotion of mammary tumor initiation and metastasis. Depletion of Gata3 in luminal tumor cells similarly regulates Fra1 and c-Fos in activation of EMT. GATA3 binds to FOSL1 (encoding FRA1) and FOS (encoding c-FOS) loci to repress FOSL1 and activate FOS transcription. Deletion of Fra1 or reconstitution of Gata3, but not reconstitution of c-Fos, in Gata3 deficient tumor cells inhibits EMT, preventing tumorigenesis and/or metastasis. In human breast cancers, GATA3 expression is negatively correlated with FRA1 and positively correlated with c-FOS. Low GATA3 and FOS, but high FOSL1, are characteristics of BLBCs. Together, these data provide the first genetic evidence indicating that loss of function of GATA3 in mammary tumor cells activates FOSL1 to promote mesenchymal traits and CSC function, while concurrently repressing FOS to lose epithelial features. We demonstrate that FRA1 is required for the activation of EMT in GATA3 deficient tumorigenesis and metastasis.
Diamond-structured nanonetwork gold as mechanical metamaterials from bottom-up approach
Authors: Siddique Suhail K. , Sadek Hassan , Wang Chi-Wei , Lee Chang-Chun , Tsai Cheng-Yuan , Chang Shou-Yi , Li Chia-Lin , Hsueh Chun-Hway , Ho Rong-Ming 
Published: June 2023

AbstractHerein, this work aims to develop a facile method for the fabrication of metallic mechanical metamaterial with a well-ordered diamond structure from a bottom-up approach using a self-assembled block copolymer for templated electrochemical deposition. By controlling the effective volume fraction of PDMS in PS-b-PDMS via solvent annealing followed by HF etching of PDMS, it is feasible to obtain nanoporous PS with diamond-structured nanochannels and used it as a template for templated electrochemical deposition. Subsequently, well-ordered nanonetwork gold (Au) can be fabricated. As evidenced by nanoindentation and micro-compression tests, the mechanical properties of the diamond-structured Au after removal of PS give the combination of lightweight and mechanically robust characteristics with an exceptionally high reduced elastic modulus of 11.9 ± 0.6 GPa and yield strength of 193 ± 11 MPa above the Hashin-Shtrikman upper bound of 72 MPa with a bending-dominated structure at equivalent density. The corresponding deformation mechanism can be elucidated by morphological observations experimentally and finite element analysis (FEA) numerically. This work demonstrates the bottom-up approach to fabricating metallic monolith with diamond structure in the nanoscale, giving a superior performance as mechanical metamaterials.
Structure of human TRPV4 in complex with GTPase RhoA
Authors: Nadezhdin Kirill D. , Talyzina Irina A. , Parthasarathy Aravind , Neuberger Arthur , Zhang David X. , Sobolevsky Alexander I. 
Published: June 2023

AbstractTransient receptor potential (TRP) channel TRPV4 is a polymodal cellular sensor that responds to moderate heat, cell swelling, shear stress, and small-molecule ligands. It is involved in thermogenesis, regulation of vascular tone, bone homeostasis, renal and pulmonary functions. TRPV4 is implicated in neuromuscular and skeletal disorders, pulmonary edema, and cancers, and represents an important drug target. The cytoskeletal remodeling GTPase RhoA has been shown to suppress TRPV4 activity. Here, we present a structure of the human TRPV4-RhoA complex that shows RhoA interaction with the membrane-facing surface of the TRPV4 ankyrin repeat domains. The contact interface reveals residues that are mutated in neuropathies, providing an insight into the disease pathogenesis. We also identify the binding sites of the TRPV4 agonist 4α-PDD and the inhibitor HC-067047 at the base of the S1-S4 bundle, and show that agonist binding leads to pore opening, while channel inhibition involves a π-to-α transition in the pore-forming helix S6. Our structures elucidate the interaction interface between hTRPV4 and RhoA, as well as residues at this interface that are involved in TRPV4 disease-causing mutations. They shed light on TRPV4 activation and inhibition and provide a template for the design of future therapeutics for treatment of TRPV4-related diseases.
Identification of protein phosphatase 4 catalytic subunit as a Wnt promoting factor in pan-cancer and Xenopus early embryogenesis
Authors: Wang YiLi , Han WonHee , Yun SeokMin , Han JinKwan 
Published: June 2023

AbstractProtein Phosphatase 4 Catalytic Subunit (PPP4C) is an evolutionarily conserved protein involved in multiple biological and pathological events, including embryogenesis, organogenesis, cellular homeostasis, and oncogenesis. However, the detailed mechanisms underlying these processes remain largely unknown. Thus, we investigated the potential correlation between PPP4C and biological processes (BPs) and canonical Wnt signaling using pan-cancer analysis and Xenopus laevis (X. laevis) embryo model. Our results indicate that PPP4C is a potential biomarker for specific cancer types due to its high diagnostic accuracy and significant prognostic correlation. Furthermore, in multiple cancer types, PPP4C-related differentially expressed genes (DEGs) were significantly enriched in pattern specification, morphogenesis, and canonical Wnt activation. Consistently, perturbation of Ppp4c in X. laevis embryos interfered with normal embryogenesis and canonical Wnt responses. Moreover, biochemical analysis of X. laevis embryos demonstrated that both endogenous and exogenous Ppp4c negatively regulated AXIN1 (Wnt inhibitor) abundance. This study provides novel insights into PPP4C roles in pattern specification and Wnt activation. The similarities in BPs and Wnt signaling regulation regarding PPP4C support the intrinsic link between tumorigenesis and early embryogenesis.
Extensive estuarine sedimentary storage of plastics from city to sea: Narragansett Bay, Rhode Island, USA
Authors: Fulfer Victoria M. , Walsh J. P. 
Published: June 2023

AbstractPlastics are an important new component of the global sedimentary system, and much concern exists about their transport, fate and impact. This study presents the first system-scale assessment of sedimentary storage of microplastic for an estuary, Narragansett Bay, RI (USA), and the measurements of shoreline and seabed sediments add to the growing body of literature demonstrating high coastal concentrations. Microplastic concentrations in sediments ranged from 396 to over 13,000 MP particles kg−1 dry sediment (DW), comparable to other shoreline and seafloor sites located near urban centers. As previously reported for fine sediment and other pollutants, estuarine plastic storage is extensive in Narragansett Bay, especially within the upper urbanized reaches. Over 16 trillion pieces of plastic weighing near 1000 tonnes is calculated to be stored in surface sediments of the Bay based on a power-law fit. This work highlights that estuaries may serve as a significant filter for plastic pollution, and this trapping may have negative consequences for these valuable, productive ecosystems but offer potential for efficient removal.
Association between dietary caffeine intake and severe headache or migraine in US adults
Authors: Zhang Lu , Yin Jiahui , Li Jinling , Sun Haiyang , Liu Yuanxiang , Yang Jiguo 
Published: June 2023

AbstractThe relationship between current dietary caffeine intake and severe headache or migraine is controversial. Therefore, we investigated the association between dietary caffeine intake and severe headaches or migraines among American adults. This cross-sectional study included 8993 adults (aged ≥ 20 years) with a dietary caffeine intake from the National Health and Nutrition Examination Surveys of America from 1999 to 2004. Covariates, including age, race/ethnicity, body mass index, poverty-income ratio, educational level, marital status, hypertension, cancer, energy intake, protein intake, calcium intake, magnesium intake, iron intake, sodium intake, alcohol status, smoking status, and triglycerides, were adjusted in multivariate logistic regression models. In US adults, after adjusting for potential confounders, a 100 mg/day increase in dietary caffeine intake was associated with a 5% increase in the prevalence of severe headache or migraine (odds ratio [OR] 1.05, 95% confidence interval [CI] 1.02–1.07). Further, the prevalence of severe headache or migraine was 42% higher with caffeine intake of ≥ 400 mg/day than with caffeine intake of ≥ 0 to < 40 mg/day (OR 1.42, 95% CI 1.16–1.75). Conclusively, dietary caffeine intake is positively associated with severe headaches or migraines in US adults.
Association between obstructive sleep apnea symptoms and gout in US population, a cross-sectional study
Authors: Gu Xi , Tang Dou , Xuan Yan , Shen Ying , Lu Lei Qun 
Published: June 2023

AbstractThe results of association between Obstructive Sleep Apnea (OSA) and gout are not consistent. Participants aged 20 years or older in the National Health and Nutrition Examination Survey (NHANES) 2007–2008 and 2015–2018 were included. Weighted univariable and multivariable logistic regressions were used to evaluate the association between OSA symptoms and gout. The subgroup and sensitivity analyses were also performed. Among the 15,947 participants in this study, the mean age was 47.8 years old, 48.87% of whom were male, 4891 had OSA symptoms, and 842 had gout. In multivariable logistic regression analyses, OSA symptoms were positively associated with gout in all models. The odds ratio (OR) was 1.315 and 95% confidence interval (CI) was 1.070–1.616 in fully adjusted model 4. In the subgroup analyses, we found a considerable interaction between OSA symptoms and gender with gout (P for interaction = 0.003). In the sensitivity analyses, the association between OSA symptoms and gout remained stable after adjustment for congestive heart failure and diuretics using. OSA symptoms were associated with an increased likelihood of gout. This association could especially be found in female participants.
Experimental characterization of the thermo-optic coefficient vs. temperature for 4H-SiC and GaN semiconductors at the wavelength of 632 nm
Authors: Rao Sandro , Mallemace Elisa D. , Faggio Giuliana , Iodice Mario , Messina Giacomo , Della Corte Francesco G. 
Published: June 2023

AbstractThe design of semiconductor-based photonic devices requires precise knowledge of the refractive index of the optical materials, a not constant parameter over the operating temperature range. However, the variation of the refractive index with the temperature, the thermo-optic coefficient, is itself temperature-dependent. A precise characterization of the thermo-optic coefficient in a wide temperature range is therefore essential for the design of nonlinear optical devices, active and passive integrated photonic devices and, more in general, for the semiconductor technology explored at different wavelengths, from the visible domain to the infrared or ultraviolet spectrum. In this paper, after an accurate ellipsometric and micro-Raman spectroscopy characterization, the temperature dependence of the thermo-optic coefficient ($$\frac{\partial n}{\partial T}$$

∂
n

∂
T

) for 4H-SiC and GaN in a wide range of temperature between room temperature to T = 500 K in the visible range spectrum, at a wavelength of λ = 632.8 nm, is experimentally evaluated. For this purpose, using the samples as a Fabry–Perot cavity, an interferometric technique is employed. The experimental results, for both semiconductors, show a linear dependence with a high determination coefficient, R2 of 0.9648 and 0.958, for 4H-SiC and GaN, respectively, in the considered temperature range.
Resistance training rejuvenates aging skin by reducing circulating inflammatory factors and enhancing dermal extracellular matrices
Authors: Nishikori Shu , Yasuda Jun , Murata Kao , Takegaki Junya , Harada Yasuko , Shirai Yuki , Fujita Satoshi 
Published: June 2023

AbstractAerobic training (AT) is suggested to be an effective anti-aging strategy for skin aging. However, the respective effects of resistance training (RT) have not been studied. Therefore, we compared the effects of AT and RT on skin aging in a 16-week intervention in 61 healthy sedentary middle-aged Japanese women. Data from 56 women were available for analysis. Both interventions significantly improved skin elasticity and upper dermal structure, and RT also improved dermal thickness. After the training intervention, expression of dermal extracellular matrix-related genes was increased in normal human primary dermal fibroblasts. AT and RT had different effects on circulating levels of factors, such as cytokines, hormones in serum, and metabolites, and RT increased dermal biglycan (BGN). To our knowledge, this is the first report to show different effects of AT and RT on skin aging and identify the key factors involved in RT-induced skin rejuvenation.
Synthesis of unsymmetrically and symmetrically functionalized disiloxanes via subsequent hydrosilylation of C≡C bonds
Authors: Szyling Jakub , Walkowiak Jędrzej , Czapik Agnieszka , Franczyk Adrian 
Published: June 2023

AbstractA selective synthesis of unsymmetrically functionalized disiloxanes via the subsequent hydrosilylation of internal alkynes in the first step, and alkynes (terminal or internal) or 1,3-diynes in the second, with 1,1,3,3-tetramethyldisiloxane (1) is presented for the first time. Using developed approaches performed in a stepwise or one-pot manner a new family of disubstituted disiloxanes was obtained which had previously been inaccessible by other synthetic methods. Moreover, symmetrically functionalized disiloxanes were obtained by direct hydrosilylation of 2 equivalents of terminal or internal alkynes with 1, showing the unique versatility of the hydrosilylation process. Three examples of symmetric disiloxanes were characterized by single crystal X-ray diffraction for the first time. As a result, a wide group of new compounds which can find potential applications as building blocks or coupling agents was obtained and characterized.
Development and validation of a nomogram to predict long-term cancer-specific survival for patients with osteosarcoma
Authors: Yu Yali , Wang Shaohua , Liu Jia , Ge Jiejie , Guan Hongya 
Published: June 2023

AbstractThe present work aimed to establish a new model to accurately estimate overall survival (OS) as well as cancer-specific survival (CSS) of osteosarcoma. Osteosarcoma cases were collected from the Surveillance, Epidemiology, and End Results (SEER) database between 2004 and 2017 and randomized as training or validation sets. Then, the OS- and CSS-related variables were discovered through multivariate Cox regression analysis to develop new nomograms to predict the 1-, 3- and 5-year OS and CSS. Besides, consistency index (C-index), decision curve analysis (DCA), along with calibration curve were adopted for assessing the predicting ability of our constructed nomograms after calibrating for 1-, 3- and 5-year OS and CSS. Altogether, 1727 osteosarcoma cases were enrolled in the present study and randomly divided as training (n = 1149, 70%) or validation (n = 576, 30%) set. As shown by univariate as well as multivariate Cox regression analyses, age, grade, T stage, M stage, surgery, chemotherapy, and histological type were identified to be the adverse factors to independently predict OS and CSS among the osteosarcoma cases. Besides, based on results of multivariate Cox regression analysis, we constructed the OS and CSS prediction nomograms. The C-index in training set was 0.806 (95% CI 0.769–0.836) for OS nomogram and 0.807 (95% CI 0.769–0.836) for CSS nomogram. In the meantime, C-index value in validation set was 0.818 (95% CI 0.789–0.847) for OS nomogram, while 0.804 (95% CI 0.773–0.835) for CSS nomogram. Besides, those calibration curves regarding the 3- and 5-year CSS of our constructed nomogram were highly consistent between the predicted values and the measurements in the training set as well as the external validation set. Our constructed nomogram outperformed the TNM stage in prediction. Our constructed nomogram is facile, creditable, and feasible; it efficiently predicts OS and CSS for osteosarcoma cases and can assist clinicians in assessing the prognosis for individuals and making decisions.
Development of a one-shot dual aptamer-based fluorescence nanosensor for rapid, sensitive, and label-free detection of periostin
Authors: Park Jonghoon , Ban Changill 
Published: June 2023

AbstractPeriostin is associated with several diseases, including cancers. Therefore, monitoring blood periostin levels is a powerful tool for diagnosing various diseases and identifying their severity. However, conventional detection methods pose several challenges, including high costs. To address these issues, we developed a novel one-shot dual aptamer-based fluorescence nanosensor for detecting periostin. The proposed nanosensor facilitates rapid, label-free, and sensitive detection of periostin using gold nanoprobes constructed by rhodamine-b isothiocyanate, PL2trunc aptamer, and gold nanoparticles and silver nanoprobes fabricated by the PL5trunc aptamer and silver nanoparticles. The two nanoprobes form a core-satellite structure by interacting with periostin, and the nanosensor detects periostin through the fluorescence regenerated by the increased proximity between them. The nanosensor successfully detected periostin with remarkable detection limits of 106.68 pM in buffer and 463.3 pM in serum-spiked conditions within 30 min without additional washing or signal amplification processes. Considering serum periostin levels in various diseases, the proposed nanosensor provides a suitable method for identifying patients with various diseases and determining disease severity. Moreover, the platform can be helpful as a practical method for on-site medical diagnosis because it can be adapted to detect other biomarkers simply by replacing the aptamer with other detection probes.
Probing the dynamical interaction of the para-sulfonato-calix[4]arene with an antifungal protein
Authors: Bartocci Alessio , Dumont Elise 
Published: June 2023

The interaction of para-sulfonato-calix[4]arene with a small antifungal protein is analyzed by molecular dynamics simulations, corroborating the presence of two main binding sites at the protein surface.
X-ray photodesorption of complex organic molecules in protoplanetary disks
Authors: Basalgète R. , Torres-Díaz D. , Lafosse A. , Amiaud L. , Féraud G. , Jeseck P. , Philippe L. , Michaut X. , Fillion J.-H. , Bertin M. 
Published: June 2023

Context. X-rays emitted from pre-main-sequence stars at the center of protoplanetary disks can induce nonthermal desorption from interstellar ices populating the cold regions of the disk. This process, known as X-ray photodesorption, needs to be quantified for complex organic molecules (COMs), including acetonitrile CH3CN, which has been detected in several disks.
Aims. The purpose of this work is to experimentally estimate the X-ray photodesorption yields of neutral species from pure CH3CN ices and from interstellar ice analogs for which CH3CN is mixed either in a CO-dominated ice or in a H2O-dominated ice.
Methods. The ices, grown in an ultrahigh vacuum chamber, were irradiated at 15 K by soft X-rays from synchrotron light (SOLEIL synchrotron) in the N K edge region (395–420 eV) and in the O K edge region (530–555 eV). X-ray photodesorption was probed in the gas phase via quadrupole mass spectrometry by monitoring the changes in the mass signals due to the X-ray irradiation of the ices. X-ray photodesorption yields were derived from the mass signals and were extrapolated to higher X-ray energies in order to provide astrophysical yields adapted to astrochemical models.
Results. X-ray photodesorption of the intact CH3CN is detected from pure CH3CN ices and from mixed 13CO:CH3CN ices, with an experimental yield of about 5 × 10−4 molecules photon−1 at 560 eV. When mixed in H2O-dominated ices, X-ray photodesorption of the intact CH3CN at 560 eV is below its detection limit, which is 10−4 molecules photon−1. Yields associated with the desorption of HCN, CH4, and CH3 are also provided. The derived astrophysical yields significantly depend on the local conditions expected in protoplanetary disks, that is, on the ice composition and on the local X-ray irradiation spectrum. They vary from ~10−4 to ~10−6 molecules photon−1 for the X-ray photodesorption of intact CH3CN from CO-dominated ices. Only upper limits varying from ~5 × 10−5 to ~5 × 10−7 molecules photon−1 could be derived for the X-ray photodesorption of intact CH3CN from H2O-dominated ices.
Conclusions. X-ray photodesorption of intact CH3CN from interstellar ices might in part explain the abundances of CH3CN observed in protoplanetary disks. The desorption efficiency is expected to vary with the local physical conditions, hence with the disk region considered.