bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duplicate transcription factorsGT1andVRS1regulate branching and fertile flower number in maize andBrachypodium distachyon
Authors: Gallagher Joseph P. , Man Jarrett , Chiaramida Adriana , Rozza Isabella , Patterson Erin L. , Powell Morgan , Schrager-Lavelle Amanda , Multani Dilbag S. , Meeley Robert , Bartlett Madelaine E. 
Published: March 2023

AbstractCrop engineering andde novodomestication using genome editing are new frontiers in agriculture. However, outside of well-studied crops and model systems, prioritizing engineering targets remains challenging. Evolution can serve as our guide, revealing high-priority genes with deeply conserved roles. Indeed,GRASSY TILLERS1(GT1),SIX-ROWED SPIKE1(VRS1), and their homologs have repeatedly been targets of selection in domestication and evolution. This repeated selection may be because these genes have an ancient, conserved role in regulating growth repression. To test this, we determined the roles ofGT1andVRS1homologs in maize (Zea mays) and the distantly related grass brachypodium (Brachypodium distachyon) using CRISPR-Cas9 gene editing and mutant analysis.GT1andVRS1have roles in floral development in maize and barley, respectively. Grass flowers are borne in branching structures called spikelets. In maize spikelets, carpels are suppressed in half of all initiated ear flowers. These spikelets can only produce single grains. We show thatgt1; vrs1-like1(vrl1) mutants have derepressed carpels in ear flowers. Importantly, these plants can produce two grains per spikelet. In brachypodium,bdgt1; bdvrl1mutants have more branches, spikelets, and flowers than wildtype plants, indicating conserved roles forGT1andVRS1homologs in growth suppression. Indeed, maizeGT1can suppress growth inArabidopsis thaliana, separated from the grasses byca. 160 million years of evolution. Thus,GT1andVRS1maintain their potency as growth regulators across vast timescales and in distinct developmental contexts. Modulating the activity of these and other conserved genes may be critical in crop engineering.
Viruses encode tRNA and anti-retron to evade bacterial immunity
Authors: Azam Aa Haeruman , Chihara Kotaro , Kondo Kohei , Nakamura Tomohiro , Ojima Shinjiro , Tamura Azumi , Yamashita Wakana , Cui Longzhu , Takahashi Yoshimasa , Watashi Koichi , Kiga Kotaro 
Published: March 2023

AbstractRetrons are bacterial genetic retroelements that encode reverse transcriptase capable of producing multicopy single-stranded DNA (msDNA) and function as antiphage defense systems. Phages employ several strategies to counter the host defense systems, but no mechanisms for evading retrons are known. Here, we show that tRNATyrand Rad (retron anti-defense) of T5 phage family inhibit the defense activity of retron 78 and a broad range of retrons, respectively. The effector protein of retron 78, ptuAB, specifically degraded tRNATyrleading abortive infection, but phage countervailed this defense by supplying tRNATyr. Rad inhibited retron function by degrading noncoding RNA, the precursor of msDNA. In summary, we demonstrated that viruses encode at least two independent strategies for overcoming bacterial defense systems: anti-defense, such as Rad, and defense canceler, like tRNA.
Identification of small molecule antivirals against HTLV-1 by targeting the hDLG1-Tax-1 protein-protein interaction
Authors: Maseko Sibusiso B. , Brammerloo Yasmine , Van Molle Inge , Sogues Adrià , Martin Charlotte , Gorgulla Christoph , Olivet Julien , Blavier Jeremy , Ntombela Thandokuhle , Delvigne Frank , Arthanari Haribabu , Salehi-Ashtiani Kourosh , Remaut Han , Ballet Steven , Volkov Alexander N. , Twizere Jean-Claude 
Published: March 2023

AbstractHuman T-cell leukemia virus type-1 (HTLV-1) is the first pathogenic retrovirus discovered in human. Although HTLV-1-induced diseases are well characterized and linked to the encoded Tax-1 oncoprotein, there is currently no strategy to target Tax-1 functions with small molecules. Here, we analysed the binding of Tax-1 to the human homolog of the drosophila discs large tumor suppressor (hDLG1/SAP97), a multi-domain scaffolding protein involved in Tax-1-transformation ability. We have solved the structures of the PDZ binding motif (PBM) of Tax-1 in complex with the PDZ1 and PDZ2 domains of hDLG1 and assessed the binding of 10 million molecules by virtual screening. Among the 19 experimentally confirmed compounds, one systematically inhibited the Tax-1-hDLG1 interaction in different biophysical and cellular assays, as well as HTLV-1 cell-to-cell transmission in a T-cell model. Thus, our work demonstrates that interactions involving Tax-1 PDZ-domains are amenable to small-molecule inhibition, which provides a framework for the design of targeted therapies for HTLV-1-induced diseases.
Neural and behavioral correlates of edible cannabis-induced poisoning: characterizing a novel preclinical model
Authors: Amissah Richard Quansah , Kayir Hakan , Talhat Malik Asfandyaar , Hassan Ahmad , Gu Yu , Johnson Ron , Urban Karolina , Khokhar Jibran Y. 
Published: March 2023

ABSTRACTAccidental exposure to Δ9-tetrahydrocannabinol (THC)-containing edible cannabis, leading to cannabis poisoning, is common in children and pets; however, the neural mechanisms underlying these poisonings remain unknown. Therefore, we examined the effects of acute edible cannabis-induced poisoning on neural activity and behavior. Adult Sprague-Dawley rats (6 males, 7 females) were implanted with electrodes in the prefrontal cortex (PFC), dorsal hippocampus (dHipp), cingulate cortex (Cg), and nucleus accumbens (NAc). Cannabis poisoning was then induced by exposure to a mixture of Nutella (6 g/kg) and THC-containing cannabis oil (20 mg/kg). Subsequently, cannabis tetrad and neural oscillations were examined 2, 4, 8, and 24 h after THC exposure. In another cohort (16 males, 15 females), we examined the effects of cannabis poisoning on learning and prepulse inhibition, and the serum and brain THC and 11-hydroxy-THC concentrations. Cannabis poisoning resulted in sex differences in brain and serum THC and 11-hydroxy-THC levels over a 24-h period. It also caused gamma power suppression in the Cg, dHipp, and NAc in a sex- and time-dependent manner. Cannabis poisoning also resulted in hypolocomotion, hypothermia, and anti-nociception in a time-dependent manner and impairments in learning and prepulse inhibition. Our results suggest that the impairments in learning and information processing may be due to the decreased gamma power in the dHipp and PFC. Additionally, most of the changes in neural activity and behavior appear 2 hours after ingestion, suggesting that interventions at or before this time might be effective in reversing or reducing the effects of cannabis poisoning.
IntracellularPlasmodiumaquaporin 2 is required for sporozoite production in the mosquito vector and malaria transmission
Authors: Bailey Alexander J. , Ukegbu Chiamaka Valerie , Giorgalli Maria , Besson Tanguy Rene Balthazar , Christophides George K. , Vlachou Dina 
Published: March 2023

AbstractMalaria remains a devastating disease and, with current measures failing to control its transmission, there is a need for novel interventions. A family of proteins that have long been pursued as potential intervention targets are aquaporins which are channels facilitating the movement of water and other solutes across membranes. We identify a new aquaporin in malaria parasites and demonstrate that it is essential for disease transmission through mosquitoes. Disruption of AQP2 in the human parasitePlasmodium falciparumand the rodent parasitePlasmodium bergheiblocks sporozoite production inside oocysts established on mosquito midguts, preventing parasite infection of salivary glands and transmission to a new host.In vivoepitope tagging of AQP2 inP. berghei, combined with immunofluorescence assays, reveals that the protein is localized in previously uncharacterized organelles found in the cytoplasm of gametocytes, ookinetes and sporozoites. The number of these organelles varies between individual parasites and lifecycle stages suggesting that they are likely part of a dynamic endolysosomal system. Phylogenetic analysis confirms that AQP2 is unique to malaria and closely related parasites and most closely related to other intracellular aquaporins. Structure prediction analyses identify several unusual features, including a large accessory extracellular loop and an arginine-to-phenylalanine substitution in the selectivity filter principally determining pore function, a unique feature not found in any aquaporins studied to date. This in conjunction with the requirement of AQP2 for malaria transmission suggests that AQP2 may be a fruitful new target of novel antimalarial interventions.
Work-integrated learning (WIL) as a part of quality education in architecture: the profession's perspective
Authors: Briant Sarah , Crowther Philip , Clifton Jennifer , Osborne Burton Lindy 
Published: March 2023

PurposeThis research argues that architecture knowledge is fragmented between the profession and academia as evidenced by long-standing conflicting opinions regarding desirable graduate attributes. Work-integrated learning (WIL) is one mode of education where these fragments should come together. This research seeks to address a missing part of that WIL model and understand the profession's view of what constitutes quality education as a United Nations (UN) Sustainability Development Goal (SDG).Design/methodology/approachUtilising a three round Delphi survey of the architecture profession engaged in WIL programs, this study reports on their perspectives of the key benefits and attributes of WIL and the value of WIL experiences as part of a quality higher education system.FindingsThe architecture profession confirmed the value of WIL programs as contributing to students developing an understanding of workplace culture and contexts. There was strong agreement that WIL experiences can be a valuable part of a quality education and enhance graduate employability. Challenges for practice included semester-based program timing, the length of engagement with practice and the lack of WIL program guidance by universities to prepare the profession for WIL experiences.Originality/valueWhile WIL has been extensively researched over the last decade, it is limited in the architecture discipline. Survey findings address the research gap in understanding the architecture profession's views as a key WIL stakeholder which is important given the rapidly changing practice environments, globalisation and the increasingly transdisciplinary context.
Blockchain adoption in agri-food supply chain management: an empirical study of the main drivers using extended UTAUT
Authors: Sharma Anandika , Sharma Anupam , Singh Rohit Kumar , Bhatia Tarunpreet 
Published: March 2023

PurposeBlockchain technology can overcome many complicated problems related to confidentiality, integrity and availability of fast and secure distributed systems in the agri-food supply chain. In emerging economies like India, blockchain application in the agri-food supply chain is still new, and their adoption is underdeveloped. This paper aims to investigate the drivers of blockchain technology adoption and their effect on the behavioral intention of stakeholders in adopting blockchain technology among various stakeholders in the agri-food supply chain. The study also develops a framework to enhance understanding of blockchain adoption in the agri-food supply chain as well as the stakeholders' motivation in seeking blockchain solutions.Design/methodology/approachConsidering the most significant aspects of blockchain adoption in the agri-food supply chain, this study attempts to develop an adoption model by using the extended unified theory of acceptance and technology model with interfirm trust and transparency as additional factors. Data was collected from a sample of 200 stakeholders in the North Indian state of Punjab. The empirical analysis was carried out using structural equation modeling in Smart PLS3.FindingsThe findings supported the developed framework and the results of SEM indicate that all the paths are supported. In particular, the findings of the study reveal that performance expectancy, effort expectancy, social influence, facilitating conditions, interfirm trust and transparency are the drivers of blockchain adoption and have a significant impact on the behavioral intention of stakeholders. Cumulatively, the results positively impact the performance of agri-food supply chain. From this study, it is found that the adoption of blockchain technology in agri-food supply chain enhances their performance.Originality/valueThe originality of the study lies in the developed framework, technology adoption will help them focus in the right direction by eliminating manual methods and converting the agri-food supply chain into a digitalization system.
A new method based on ensemble time series for fast and accurate clustering
Authors: Ghorbanian Ali , Razavi Hamideh 
Published: March 2023

PurposeThe common methods for clustering time series are the use of specific distance criteria or the use of standard clustering algorithms. Ensemble clustering is one of the common techniques used in data mining to increase the accuracy of clustering. In this study, based on segmentation, selecting the best segments, and using ensemble clustering for selected segments, a multistep approach has been developed for the whole clustering of time series data.Design/methodology/approachFirst, this approach divides the time series dataset into equal segments. In the next step, using one or more internal clustering criteria, the best segments are selected, and then the selected segments are combined for final clustering. By using a loop and how to select the best segments for the final clustering (using one criterion or several criteria simultaneously), two algorithms have been developed in different settings. A logarithmic relationship limits the number of segments created in the loop.FindingAccording to Rand's external criteria and statistical tests, at first, the best setting of the two developed algorithms has been selected. Then this setting has been compared to different algorithms in the literature on clustering accuracy and execution time. The obtained results indicate more accuracy and less execution time for the proposed approach.Originality/valueThis paper proposed a fast and accurate approach for time series clustering in three main steps. This is the first work that uses a combination of segmentation and ensemble clustering. More accuracy and less execution time are the remarkable achievements of this study.
Cross-cultural cognitive conditions and gender differences in the entrepreneurial activity during the COVID-19 pandemic
Authors: Pinho José Carlos , Sá Elisabete 
Published: March 2023

Purpose
The purpose of this study is to advance knowledge on the gender-specific conditions that lead to higher entrepreneurial activity (EA) in challenging contexts by examining the interdependencies between several cognitive dimensions (perceived opportunities, perceived capabilities, being undeterred by fear of failure, knowing an entrepreneur who started a business because of the pandemic) and one cultural dimension (masculinity) and their relationship with EA of women and men during the COVID-19 pandemic.

Design/methodology/approach
Using data from the Global Entrepreneurship Monitor’s special report “Women’s Entrepreneurship 2020/21: Thriving Through Crisis” for 43 countries, this study uses fuzzy-set qualitative comparative analysis to assess complex causal conditions.

Findings
Results revealed six different causal configurations leading to high female EA and the same number leading to high male EA during the COVID-19 pandemic. Although half of these configurations are gender specific, this study found several similarities in the complex conditions leading to the same outcome for women and men. The specific dimensions of perceived opportunities and knowing an entrepreneur who started a business because of the pandemic were crucial for both genders. Still, the latter is particularly important for women. Masculinity orientation was only relevant in the male sample.

Originality/value
Despite the remarkable growth of the research on gender and entrepreneurship over the past years, there is still limited knowledge on what leads to women and men’s EA in times of crisis. This study's results advance the understanding of gender differences and similarities regarding the complex conditions that favour EA under highly challenging circumstances such as the ones imposed by the COVID-19 pandemic crisis.
Three-parameter interval grey number dynamic TOPSIS method based on comprehensive similarity
Authors: Li Meijuan , Zhang Jiarong , Shen Zijie 
Published: March 2023

PurposeThree-parameter interval grey numbers (TPIGNs) have been extensively studied as an extended form of interval numbers. However, most existing TPIGN multi-attribute decision-making methods only consider the similarity of positions, ignore the similarity of developmental directions and focus primarily on static evaluation. To address these limitations, in this study, the authors propose a dynamic technique for order preference by similarity to an ideal solution (TOPSIS) based on modified Jaccard similarity and angle similarity for TPIGNs.Design/methodology/approachFirst, the authors extend Jaccard similarity to a TPIGN environment to represent positional similarity. A simple example is provided to illustrate the limitations of the traditional Jaccard similarity. Then, the authors introduce an angle similarity measure to represent developmental directional similarity. Finally, a dynamic TOPSIS model is constructed by incorporating time-series data into conventional two-dimensional static data. Stage weights are obtained by an objective function designed to maximize the amount and minimize the fluctuation of decision information. A quadratic weighting method is adopted to derive the overall evaluation value of alternatives.FindingsTo evaluate the effectiveness of the proposed method, this study takes the pre-assessment of ice disaster and the selection of cooperative enterprises as examples. The authors then provide the results of comparative and sensitivity analyses, which demonstrate the effectiveness and flexibility of the proposed method.Originality/valueThe proposed TOPSIS method in a TPIGN environment can take a more holistic and dynamic perspective for decision-making, which helps mitigate the limitations of single-perspective or static evaluations.
Proposed active flow control enabled hybrid tilt propeller/tilt wing aircraft
Authors: Taubert Lutz , Kay Garrett , Wygnanski Israel , Ol Michael 
Published: March 2023

Purpose
This paper aims to address shortcomings of current tiltrotor designs, such as the small aspect ratio of the wings, large download and the close proximity of the rotor tips. It also aims to avoid the complex transition of tiltrotors to normal airplane mode.

Design/methodology/approach
This design combines tiltrotor and tiltwing aircraft designs into a hybrid that is augmented by active flow control, using a gimbaled channel wing for attitude control in hover.

Findings
The proposed hybrid design is based on experimental results of components that were tested individually for potential use in hover and steep ascend from a stationary position.

Originality/value
This research was inspired by the extremely short take-off of the V-22, when its rotors were tilted forward. It combines several design approaches in a unique way to achieve extremely short take-off capabilities combined with high-speed and reduced maintenance costs.
The effects of foreign currency exposure and Sharī’ah-compliant status on financial hedging strategy
Authors: Abdul-Rahim Ruzita , Abd Wahab Adilah , Hudaib Mohammad 
Published: March 2023

Purpose
Drawing upon underinvestment theory and clientele effect hypothesis, this paper aims to examine the effects of foreign currency (forex) exposure and Shari’ah-compliant status on firms’ financial hedging strategy.

Design/methodology/approach
Based on data of 250 nonfinancial firms listed on Bursa Malaysia from 2010 to 2018 (2,250 firm-year observations), the authors test the impact of forex exposure based on a vector of foreign-denominated cash flows (FCF) indicators and firms’ Sharīʿah-compliant status on two proxies of financial hedging decisions, namely, the ratio of the notional value of currency derivatives to total assets and a binomial measure of hedging status. The hedging decision models are estimated using panel logistic regression and system generalized method of moments.

Findings
The results indicate significant positive effects of the forex exposure indicators on firms’ propensity to hedge. However, the impact of forex exposure is most prevalent via total FCF. The results also reveal significant positive effects of Sharīʿah-compliant status on firms’ propensity to hedge but its negative impacts on the value of currency derivatives they use. The results suggest that Sharīʿah-compliant firms refrain from engaging in currency derivatives to avoid riba’ and subsequently subdue the clientele effect. However, when the forex exposure reaches higher levels, engagement in currency derivatives becomes a matter of tentative necessity (dharurat).

Research limitations/implications
This study relies exclusively on the disclosure of foreign currency risk and management data in the annual reports of listed companies. Consequently, this limits the sample size to only those nonfinancial listed companies with complete data for the study period. Also, since none of the companies reports using Sharīʿah-compliant derivatives, the authors thus assume that they use derivative instruments that tolerate “riba.”

Practical implications
Given the significance of forex exposure on hedging decisions, the accounting profession must strictly adopt FRS 7 and FRS 139 for all listed firms to avoid market scrutiny and sustain their clientele. The results also call for the Islamic market regulators to include mandatory disclosure of conventional currency derivatives in screening firms for clearly prohibited activities to help enhance the credibility of its Islamic financial market.

Originality/value
Due to difficulty accessing relevant cash flow data, the study is among the few studies that measure forex exposure using FCF and test more proxy indicators. This study is perhaps the first to examine the Shari’ah perspective on currency derivatives in corporate forex risk management.
Students' extrinsic and intrinsic motivation improvements in learning defense engineering based on project-based learning
Authors: Deng Yanyao , Shi Chao 
Published: March 2023

PurposeThis study aims to evaluate student motivation before and after the summer internship, in other words, to evaluate whether the summer internship affects male and female motivations differently.Design/methodology/approachInvestigating whether the motivation score predicts grade point average was included by adopting a quantitative methodology. The defense engineering summer internship project provides candidates who learned defense engineering knowledge after the project an opportunity to generate motivation of involving in defense engineering discipline and industry.FindingsThis study found a marginal significant main effect on extrinsic motivation – introjected (EMIN) and intrinsic motivation to experience stimulation (IMS) in a summer internship. Furthermore, the study reveals that amotivation construct significantly predicted gender and previous academic performance (GPA) pre-summer internship, and amotivation, Extrinsic motivation – external regulation (EME) and extrinsic motivation – introjection (EMIN) significantly predicted GPA post-summer internship.Originality/valueThe project was done with support from the U.S. Department of defense grant N00014-19-1-2728. These findings are investigated from the project.