bluuebunny/crossref_metadata_2025_split huggingface dataset:
Listening to the patients’ voice: a conceptual framework of the walking experience
Authors: Delgado-Ortiz Laura , Polhemus Ashley , Keogh Alison , Sutton Norman , Remmele Werner , Hansen Clint , Kluge Felix , Sharrack Basil , Becker Clemens , Troosters Thierry , Maetzler Walter , Rochester Lynn , Frei Anja , Puhan Milo A , Garcia-Aymerich Judith 
Published: February 2023

Abstract

Background
walking is crucial for an active and healthy ageing, but the perspectives of individuals living with walking impairment are still poorly understood.

Objectives
to identify and synthesise evidence describing walking as experienced by adults living with mobility-impairing health conditions and to propose an empirical conceptual framework of walking experience.

Methods
we performed a systematic review and meta-ethnography of qualitative evidence, searching seven electronic databases for records that explored personal experiences of walking in individuals living with conditions of diverse aetiology. Conditions included Parkinson’s disease, multiple sclerosis, chronic obstructive pulmonary disease, hip fracture, heart failure, frailty and sarcopenia. Data were extracted, critically appraised using the NICE quality checklist and synthesised using standardised best practices.

Results
from 2,552 unique records, 117 were eligible. Walking experience was similar across conditions and described by seven themes: (i) becoming aware of the personal walking experience, (ii) the walking experience as a link between individuals’ activities and sense of self, (iii) the physical walking experience, (iv) the mental and emotional walking experience, (v) the social walking experience, (vi) the context of the walking experience and (vii) behavioural and attitudinal adaptations resulting from the walking experience. We propose a novel conceptual framework that visually represents the walking experience, informed by the interplay between these themes.

Conclusion
a multi-faceted and dynamic experience of walking was common across health conditions. Our conceptual framework of the walking experience provides a novel theoretical structure for patient-centred clinical practice, research and public health.
Apolipoprotein E gene variants shape the association between dietary fibre intake and cognitive decline risk in community-dwelling older adults
Authors: Unión-Caballero Andrea , Meroño Tomás , Andrés-Lacueva Cristina , Hidalgo-Liberona Nicole , Rabassa Montserrat , Bandinelli Stefania , Ferrucci Luigi , Fedecostante Massimiliano , Zamora-Ros Raúl , Cherubini Antonio 
Published: February 2023

Abstract

Background
healthy dietary patterns have been associated with lower risk for age-related cognitive decline. However, little is known about the specific role of dietary fibre on cognitive decline in older adults.

Objective
this study aimed to examine the association between dietary fibre and cognitive decline in older adults and to assess the influence of genetic, lifestyle and clinical characteristics in this association.

Design and participants
the Invecchiare in Chianti, aging in the Chianti area study is a cohort study of community-dwelling older adults from Italy. Cognitive function, dietary and clinical data were collected at baseline and years 3, 6, 9 and 15. Our study comprised 848 participants aged ≥ 65 years (56% female) with 2,038 observations.

Main outcome and measures
cognitive decline was defined as a decrease ≥3 units in the Mini-Mental State Examination score during consecutive visits. Hazard ratios for cognitive decline were estimated using time-dependent Cox regression models.

Results
energy-adjusted fibre intake was not associated with cognitive decline during the 15-years follow-up (P &gt; 0.05). However, fibre intake showed a significant interaction with Apolipoprotein E (APOE) haplotype for cognitive decline (P = 0.02). In participants with APOE-ɛ4 haplotype, an increase in 5 g/d of fibre intake was significantly associated with a 30% lower risk for cognitive decline. No association was observed in participants with APOE-ɛ2 and APOE-ɛ3 haplotypes.

Conclusions and relevance
dietary fibre intake was not associated with cognitive decline amongst older adults for 15 years of follow-up. Nonetheless, older subjects with APOE-ɛ4 haplotype may benefit from higher fibre intakes based on the reduced risk for cognitive decline in this high-risk group.
Development of oxidoreductases for amino acid quantification and mutagenesis techniques for heterologous soluble expression: screening and selection strategies
Authors: Matsui Daisuke 
Published: January 2023

ABSTRACT
The high stereo- and substrate specificities of enzymes have been utilized for microdetermination of amino acids. Here, I review the discovery of l-Arg oxidase from Pseudomonas sp. TPU 7192, l-Lys oxidase/decarboxylase from Burkholderia sp. AIU 395, and enzymes showing apparent l-His oxidase activity from Achromobacter sp. TPU 5009. I also discuss screening and uses of the selective enzymes for microdetermination of amino acids. In addition, functional modifications of l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813, l-Trp dehydrogenase from Nostoc punctiforme ATCC 29133, and l-Lys ε-oxidase from Marinomonas mediterranea NBRC 103028 by directed evolution are reviewed. Finally, I review the rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity—this process enables the wider use of natural enzymes.
Exploring pharmacological active ingredients of traditional Chinese medicine by pharmacotranscriptomic map in ITCM
Authors: Tian Saisai , Zhang Jinbo , Yuan Shunling , Wang Qun , Lv Chao , Wang Jinxing , Fang Jiansong , Fu Lu , Yang Jian , Zu Xianpeng , Zhao Jing , Zhang Weidong 
Published: January 2023

AbstractWith the emergence of high-throughput technologies, computational screening based on gene expression profiles has become one of the most effective methods for drug discovery. More importantly, profile-based approaches remarkably enhance novel drug–disease pair discovery without relying on drug- or disease-specific prior knowledge, which has been widely used in modern medicine. However, profile-based systematic screening of active ingredients of traditional Chinese medicine (TCM) has been scarcely performed due to inadequate pharmacotranscriptomic data. Here, we develop the largest-to-date online TCM active ingredients-based pharmacotranscriptomic platform integrated traditional Chinese medicine (ITCM) for the effective screening of active ingredients. First, we performed unified high-throughput experiments and constructed the largest data repository of 496 representative active ingredients, which was five times larger than the previous one built by our team. The transcriptome-based multi-scale analysis was also performed to elucidate their mechanism. Then, we developed six state-of-art signature search methods to screen active ingredients and determine the optimal signature size for all methods. Moreover, we integrated them into a screening strategy, TCM-Query, to identify the potential active ingredients for the special disease. In addition, we also comprehensively collected the TCM-related resource by literature mining. Finally, we applied ITCM to an active ingredient bavachinin, and two diseases, including prostate cancer and COVID-19, to demonstrate the power of drug discovery. ITCM was aimed to comprehensively explore the active ingredients of TCM and boost studies of pharmacological action and drug discovery. ITCM is available at http://itcm.biotcm.net.
Role of poFUT1 and O-fucosylation in placental angiogenesis
Authors: Liang Caixia , Li Yaqi , Qin Huamin , Ramzan Muhammad Noman , Wang Hao , Liu Shuai , Yan Qiu 
Published: February 2023

AbstractTrophoblast cells are critical to placental angiogenesis in the first trimester of pregnancy. Dysfunction of trophoblast leads to defective vascular remodeling and impaired angiogenesis, which is believed as the major cause of placental insufficiency and pregnancy failure. Protein O-fucosyltransferase 1 (poFUT1) is mainly responsible for O-fucosylated glycan biosynthesis on glycoproteins, and poFUT1 deficiency causes embryonic lethality in mice. However, the expression and function of poFUT1 in trophoblast-mediated human placental vessel formation remain unclear. In the current study, we showed that fewer blood vessels were observed in the villi and decidua of miscarriage patients than in normal pregnancy women. The expression of poFUT1 was decreased in the trophoblast cells of miscarriage patients compared with normal pregnancy women. Employing HTR/SVneo cells and an in vivo chorioallantoic membrane assay, we demonstrated that poFUT1 promoted the proliferation, migration ability, and angiogenesis potential of trophoblast cells. The results also indicated that poFUT1 upregulated O-fucosylation on uPA, facilitated the binding of uPA and uPAR, activated the RhoA signaling pathway, and further enhanced the angiogenic capacity of trophoblast cells. Our study provides new evidence for a relationship between poFUT1/O-fucosylation and placental angiogenesis. These findings may provide potential diagnostic biomarkers and targeted therapies for miscarriage patients.
Impact of hospital volume in liver surgery on postoperative mortality and morbidity: nationwide study
Authors: Magnin Josephine , Bernard Alain , Cottenet Jonathan , Lequeu Jean-Baptiste , Ortega-Deballon Pablo , Quantin Catherine , Facy Olivier 
Published: February 2023

Abstract

Background
This nationwide retrospective study was undertaken to evaluate impact of hospital volume and influence of liver transplantation activity on postoperative mortality and failure to rescue after liver surgery.

Methods
This was a retrospective study of patients who underwent liver resection between 2011 and 2019 using a nationwide database. A threshold of surgical activities from which in-hospital mortality declines was calculated. Hospitals were divided into high- and low-volume centres. Main outcomes were in-hospital mortality and failure to rescue.

Results
Among 39 286 patients included, the in-hospital mortality rate was 2.8 per cent. The activity volume threshold from which in-hospital mortality declined was 25 hepatectomies. High-volume centres (more than 25 resections per year) had more postoperative complications but a lower rate of in-hospital mortality (2.6 versus 3 per cent; P &lt; 0.001) and failure to rescue (5 versus 6.3 per cent; P &lt; 0.001), in particular related to specific complications (liver failure, biliary complications, vascular complications) (5.5 versus 7.6 per cent; P &lt; 0.001). Liver transplantation activity did not have an impact on these outcomes.

Conclusion
From more than 25 liver resections per year, rates of in-hospital mortality and failure to rescue declined. Management of specific postoperative complications appeared to be better in high-volume centres.
Call to Action: Prioritizing the Use of Inclusive, Nonstigmatizing Language in Scientific Communications
Authors: Bares Sara H , Marcelin Jasmine R , Blumenthal Jill , Sax Paul E 
Published: January 2023

Abstract
The language we use in our scientific communications can either empower or stigmatize the people we study and care for. Clinical Infectious Diseases is committed to prioritizing the use of inclusive, nonstigmatizing language in published manuscripts. We hereby call upon submitting authors, reviewers, and editors to do the same.
Cardioprotective efficacy of limb remote ischaemic preconditioning in rats: discrepancy between a meta-analysis and a three-centre in vivo study
Authors: Sayour Nabil V , Brenner Gábor B , Makkos András , Kiss Bernadett , Kovácsházi Csenger , Gergely Tamás G , Aukrust Sverre Groever , Tian Huimin , Zenkl Viktória , Gömöri Kamilla , Szabados Tamara , Bencsik Péter , Heinen Andre , Schulz Rainer , Baxter Gary F , Zuurbier Coert J , Vokó Zoltán , Ferdinandy Péter , Giricz Zoltán 
Published: January 2023

Abstract

Aims
Remote ischaemic preconditioning (RIPC) is a robust cardioprotective intervention in preclinical studies. To establish a working and efficacious RIPC protocol in our laboratories, we performed randomized, blinded in vivo studies in three study centres in rats, with various RIPC protocols. To verify that our experimental settings are in good alignment with in vivo rat studies showing cardioprotection by limb RIPC, we performed a systematic review and meta-analysis. In addition, we investigated the importance of different study parameters.

Methods and results
Male Wistar rats were subjected to 20–45 min cardiac ischaemia followed by 120 min reperfusion with or without preceding RIPC by 3 or 4 × 5−5 min occlusion/reperfusion of one or two femoral vessels by clamping, tourniquet, or pressure cuff. RIPC did not reduce infarct size (IS), microvascular obstruction, or arrhythmias at any study centres. Systematic review and meta-analysis focusing on in vivo rat models of myocardial ischaemia/reperfusion injury with limb RIPC showed that RIPC reduces IS by 21.28% on average. In addition, the systematic review showed methodological heterogeneity and insufficient reporting of study parameters in a high proportion of studies.

Conclusion
We report for the first time the lack of cardioprotection by RIPC in rats, assessed in individually randomized, blinded in vivo studies, involving three study centres, using different RIPC protocols. These results are in discrepancy with the meta-analysis of similar in vivo rat studies; however, no specific methodological reason could be identified by the systematic review, probably due to the overall insufficient reporting of several study parameters that did not improve over the past two decades. These results urge for publication of more well-designed and well-reported studies, irrespective of the outcome, which are required for preclinical reproducibility, and the development of clinically translatable cardioprotective interventions.
Production Networks And International Fiscal Spillovers
Authors: Devereux Michael B , Gente Karine , Yu Changhua 
Published: January 2023

Abstract
This paper analyses the impact of fiscal spending shocks in a dynamic, multi-country model with international production networks. The response of real gross domestic product to a fiscal spending shock can be decomposed into a direct effect, income effect and price effect. The direct effect depends only on input-output linkages, while the price effect is zero in the aggregate. We apply this decomposition to the Eurozone, and find that fiscal spillovers from Germany and the core Eurozone countries can be large, and within the range of empirical estimates. Without international production networks, spillovers would be significantly smaller. In an empirical application, using the decomposition, we find results strongly consistent with the model.
A mitral valve repair update: simple, intuitive and physiological techniques, using running sutures (part I)
Authors: Kofidis Theo 
Published: February 2023

Abstract
Today’s mitral valve techniques are pluralistic, and the result of variable contributions over decades. However, they are often associated with subjectivity, and inter-surgeon variability, as well as differing outcomes. Here, we propose a set of conceptual techniques, which aim to simplify and standardize mitral valve repair, of which some we have already verified in practice.
Family physicians’ knowledge levels about elder abuse and neglect in a province of Türkiye and hesitations in reporting
Authors: Durmaz Adem , Yılmaz Muammer 
Published: February 2023

Abstract

Background
This study aimed to investigate the knowledge, attitudes and reservations of family physicians (FPs) in reporting elderly abuse and neglect (EAN).

Methods
Our cross-sectional study was conducted with 161 FPs in Kütahya, a city in Türkiye. A questionnaire including demographic characteristics, the frequency with which Elder Abuse Suspicion Index (EASI) questions were asked and knowledge level of EAN was applied to the FPs.

Results
Only about a quarter (24.2%) of the FPs felt competent regarding EAN. The rate of participants who had encountered EAN victims before was 46%. Of these, 40% reported psychological violence, 24.3% reported physical and psychological violence and 21.6% reported only physical violence. FPs also reported that inconsistency in anamnesis (87.0%), inconsistency between anamnesis and physical examination findings (85.1%) and frequent visits to the emergency department (59.6%) raised suspicion about EAN. It was also observed that 68.9% of the FPs knew that healthcare professionals had a legal obligation to report elder abuse. Only 23.0% of the FPs who encountered cases of violence and abuse stated that they had made a legal report. Among the FPs who did not report, 40.4% stated that they did not report because they had some reservations or did not know how to do so (35.1%).

Conclusion
Due to a lack of knowledge and some concerns, the rate of reporting EAN to judicial authorities was also low. FPs were not aware of the importance of EAN in terms of elderly health.
The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato
Authors: Xing Shihan , Li Ruijie , Zhao Haoqiang , Zhai Hong , He Shaozhen , Zhang Huan , Zhou Yuanyuan , Zhao Ning , Gao Shaopei , Liu Qingchang 
Published: February 2023

AbstractCarotenoid is a tetraterpene pigment beneficial for human health. Although the carotenoid biosynthesis pathway has been extensively studied in plants, relatively little is known about their regulation in sweet potato. Previously, we conducted the transcriptome database of differentially expressed genes between the sweet potato (Ipomoea batatas) cultivar ‘Weiduoli’ and its high-carotenoid mutant ‘HVB-3’. In this study, we selected one of these candidate genes, IbNAC29, for subsequent analyses. IbNAC29 belongs to the plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factor family. Relative IbNAC29 mRNA level in the HVB-3 storage roots was ~1.71-fold higher than Weiduoli. Additional experiments showed that the contents of α-carotene, lutein, β-carotene, zeaxanthin, and capsanthin are obviously increased in the storage roots of transgenic sweet potato plants overexpressing IbNAC29. Moreover, the levels of carotenoid biosynthesis genes in transgenic plants were also up-regulated. Nevertheless, yeast one-hybrid assays indicated that IbNAC29 could not directly bind to the promoters of these carotenoid biosynthesis genes. Furthermore, the level of IbSGR1 was down-regulated, whose homologous genes in tomato can negatively regulate carotene accumulation. Yeast three-hybrid analysis revealed that the IbNAC29-IbMYB1R1-IbAITR5 could form a regulatory module. Yeast one-hybrid, electrophoretic mobility shift assay, quantitative PCR analysis of chromatin immunoprecipitation and dual-luciferase reporter assay showed that IbAITR5 directly binds to and inhibits the promoter activity of IbSGR1, up-regulating carotenoid biosynthesis gene IbPSY. Taken together, IbNAC29 is a potential candidate gene for the genetic improvement of nutritive value in sweet potato.
SIM-SR—Semiotic Inspection Method Mediated by Screen Reader: Analysing the Benefits of Bringing Communicability to the Accessibility Evaluation Table
Authors: Pedroso Carvalho Lucas , Ribeiro de Souza Joana Gabriela , Oliveira Prates Raquel , Pimenta Freire André 
Published: February 2023

Abstract
Accessibility--&gt; inspection methods provide an important complement to user evaluations in the development of interactive systems. However, many inspection methods tend to focus on violations of guidelines and are limited in conveying the impact of such problems when delivering their results. Drawing from the theoretical framework of Semiotic Engineering of Human–Computer Interaction, this paper presents SIM-SR—Semiotic Inspection Method Mediated by Screen Reader and an investigation to collect indicators about the method’s applicability and insights it can generate. In order to assess its applicability, we conducted three case studies employing the method, analysing how aspects of accessibility and communicability were approached and results generated. Regarding SIM-SR insights, we used WCAG 2.1 criteria to discuss how communicative breakdowns relate to accessibility guidelines and their impact. Our results show that SIM-SR went beyond identifying guidelines violations and led evaluators to consider the impact of accessibility issues on the system’s communicability and different levels of communicative breakdowns that users with visual disabilities could experience during interaction.
Transiently silent acquired antimicrobial resistance: an emerging challenge in susceptibility testing
Authors: Wagner Theresa Maria , Howden Benjamin Peter , Sundsfjord Arnfinn , Hegstad Kristin 
Published: January 2023

AbstractAcquisition and expression of antimicrobial resistance (AMR) mechanisms in bacteria are often associated with a fitness cost. Thus, evolutionary adaptation and fitness cost compensation may support the advance of subpopulations with a silent resistance phenotype when the antibiotic selection pressure is absent. However, reports are emerging on the transient nature of silent acquired AMR, describing genetic alterations that can change the expression of these determinants to a clinically relevant level of resistance, and the association with breakthrough infections causing treatment failures. This phenomenon of transiently silent acquired AMR (tsaAMR) is likely to increase, considering the overall expansion of acquired AMR in bacterial pathogens. Moreover, the augmented use of genotypic methods in combination with conventional phenotypic antimicrobial susceptibility testing (AST) will increasingly enable the detection of genotype and phenotype discrepancy. This review defines tsaAMR as acquired antimicrobial resistance genes with a corresponding phenotype within the wild-type distribution or below the clinical breakpoint for susceptibility for which genetic alterations can mediate expression to a clinically relevant level of resistance.References to in vivo resistance development and therapeutic failures caused by selected resistant subpopulations of tsaAMR in Gram-positive and Gram-negative pathogens are given. We also describe the underlying molecular mechanisms, including alterations in the expression, reading frame or copy number of AMR determinants, and discuss the clinical relevance concerning challenges for conventional AST.
Lentilactobacillus hilgardiiH-50 strongly inhibits lipopolysaccharide-induced inflammatory responses in mouse splenocytes via its specific surface layer proteins
Authors: Yamaguchi Tomofumi , Yasui Kenta , Fujii Sotaro , Ichikawa Kennosuke , Suzuki Takuya , Sambongi Yoshihiro , Yamamoto Yoshinari 
Published: February 2023

AbstractAimsCertain lactic acid bacteria (LAB) are known to have anti-inflammatory effects; however, hiochi bacteria, which are taxonomically classified as LAB and known to spoil a traditional Japanese alcoholic beverage, have not been studied in the same context. The aim of this study is to investigate the anti-inflammatory effects of hiochi bacteria strains and the underlying mechanisms.Methods and ResultsWe screened 45 strains of hiochi bacteria for anti-inflammatory effects and found that Lentilactobacillus hilgardii H-50 strongly inhibits lipopolysaccharide (LPS)-induced secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in mouse splenocytes. This inhibition is attributed to its specific surface layer proteins (SLPs), which directly bind to LPS.ConclusionsThe L. hilgardii H-50 strain exerts anti-inflammatory effects through its SLPs.