bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of aggression level in adolescent girls with classical congenital adrenal hyperplasia
Authors: Isik Recep Oğuzhan , Kizilcan Cetin Sirmen , Cetinkaya Semra , Berberoglu Merih , Siklar Zeynep , Özsu Elif , Aycan Zehra 
Published: June 2023

AbstractObjectiveIn patients with classical congenital adrenal hyperplasia (CAH), virilization affects the brain and external genitalia due to antenatal androgen exposure. There are few studies on how the effects of androgens on brain virilization are reflected in behavior. However, there is no study focused on the adolescence period. The aim of this study was to evaluate the level of aggression in adolescent girls with classical CAH (due to 21 hydroxylase and 11β hydroxylase deficiency) and to investigate the disease‐related factors that may affect aggression.DesignTwenty female and 20 male patients aged 13–20 years, diagnosed with classical CAH, with 21 hydroxylase deficiency and 11β hydroxylase deficiency, and 20 healthy girls and 20 boys from the same age group were included. The Buss‐Perry Aggression Scale (BPAS), which consists of four subgroups measuring physical aggression, verbal aggression, hostility, and angry behaviors, was used.ResultsThe ages of the male and female patients with CAH were 16.30 ± 2.65 and 16.60 ± 2.41 years, respectively. Total aggression scale scores were 73.3 ± 14.6 in adolescent girls with CAH, 74.1 ± 11.2 in healthy girls, 71.5 ± 14.8 in boys with CAH, and 75.3 ± 14.5 in healthy boys (p > .05). There was no difference between the subscale scores of patients and healthy adolescents. Aggression scores in adolescents with CAH increased significantly with age.ConclusionsIn this study, we found no difference between the aggression scores of adolescents with classical CAH compared to their healthy peers. The total aggression score and subscale were similar in unaffected female adolescents.
Modulation of gene expression and bone formation by expanded and dense polytetrafluoroethylene membranes during guided bone regeneration: An experimental study
Authors: Turri Alberto , Omar Omar , Trobos Margarita , Thomsen Peter , Dahlin Christer 
Published: June 2023

AbstractBackgroundNonresorbable membranes promote bone formation during guided bone regeneration (GBR), yet the relationships between membrane properties and molecular changes in the surrounding tissue are largely unknown.AimTo compare the molecular events in the overlying soft tissue, the membrane, and the underlying bone defect during GBR using dual‐layered expanded membranes versus dense polytetrafluoroethylene (PTFE) membranes.Materials and MethodsRat femur defects were treated with either dense PTFE (d‐PTFE) or dual‐layered expanded PTFE (dual e‐PTFE) or left untreated as a sham. Samples were collected after 6 and 28 days for gene expression, histology, and histomorphometry analyses.ResultsThe two membranes promoted the overall bone formation compared to sham. Defects treated with dual e‐PTFE exhibited a significantly higher proportion of new bone in the top central region after 28 days. Compared to that in the sham, the soft tissue in the dual e‐PTFE group showed 2‐fold higher expression of genes related to regeneration (FGF‐2 and FOXO1) and vascularization (VEGF). Furthermore, compared to cells in the d‐PTFE group, cells in the dual e‐PTFE showed 2.5‐fold higher expression of genes related to osteogenic differentiation (BMP‐2), regeneration (FGF‐2 and COL1A1), and vascularization (VEGF), in parallel with lower expression of proinflammatory cytokines (IL‐6 and TNF‐α). Multiple correlations were found between the molecular activities in membrane‐adherent cells and those in the soft tissue.ConclusionSelective surface modification of the two sides of the e‐PTFE membrane constitutes a novel means of modulating the tissue response and promoting bone regeneration.
Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis
Authors: Li Siqi , Sun Fei , Wei Yiping , Nie Yong , Wu Xiaolei , Hu Wenjie 
Published: June 2023

AbstractObjectivesThis study aimed to investigate the relationship between microbial communities and the severity of peri‐implant mucosal bleeding in peri‐implant mucositis.Materials and MethodsSubmucosal plaque samples were collected from 54 implants divided into the healthy implant (HI) group, peri‐implant mucositis (PM) group, and peri‐implantitis (PI) group. Sequencing of 16S rRNA was performed using the Illumina MiSeq platform. Alpha diversity (i.e., Shannon and Chao index) and beta diversity were used to measure microbial diversity within and between microbial communities, respectively. Differences in microbial taxa between groups were assessed via linear discriminate analysis effect size. Correlation between the modified sulcus bleeding index (mSBI) and microbial dysbiosis index (MDI) was examined using Spearman correlation analysis and linear models.ResultsThe submucosal bacterial richness (Chao index) was positively correlated with the mean mSBI in the PM group. As the mean mSBI increased in the PM group, the beta diversity became closer to that of the PI group. In the PM group, the abundances of 47 genera were significantly correlated with the mean mSBI, and the MDI was positively associated with the mean mSBI. Fourteen of the forty‐seven genera were discriminative taxa between the HI and PI groups, and the abundances of these biomarkers became closer to those in the PI group in the progression of peri‐implant disease.ConclusionsA higher mSBI value corresponded to a higher risk of microbial dysbiosis in peri‐implant mucositis. The biomarkers identified may be useful for monitoring the progression of peri‐implant disease.
Sustained weight loss with semaglutide once weekly in patients without type 2 diabetes and post‐bariatric treatment failure
Authors: Lautenbach Anne , Kantowski Tobias , Wagner Jonas , Mann Oliver , Stoll Fabian , Aberle Jens 
Published: June 2023

SummaryAbout 20%–25% of patients experience weight regain (WR) or insufficient weight loss (IWL) following bariatric surgery (BS). Therefore, we aimed to retrospectively assess the effectiveness of adjunct treatment with semaglutide in patients without type 2 diabetes (T2D) with post‐bariatric treatment failure over a 12 months period. Post‐bariatric patients without T2D with WR or IWL (n = 29) were included in the analysis. The primary endpoint was weight loss 12 months after initiation of adjunct treatment. Secondary endpoints included change in body mass index, HbA1c, lipid profile, high sensitive C‐reactive protein and liver enzymes. Total weight loss during semaglutide treatment added up to 14.7% ± 8.9% (mean ± SD, p < .001) after 12 months. Categorical weight loss was >5% in 89.7% of patients, >10% in 62.1% of patients, >15% in 34.5% of patients, >20% in 24.1% of patients and > 25% in 17.2% of patients. Adjunct treatment with semaglutide resulted in sustained weight loss regardless of sex, WR or IWL and type of surgery. Among patients with prediabetes (n = 6), 12 months treatment led to normoglycemia in all patients (p < .05). Treatment options to manage post‐bariatric treatment failure are scarce. Our results imply a clear benefit of adjunct treatment with semaglutide in post‐bariatric patients over a 12 months follow‐up period.
Macroprudential policy leakage: Evidence from shadow banking activities of Chinese enterprises
Authors: Lin Guiting , Ouyang Alice Y. 
Published: June 2023

AbstractUsing entrusted loan data of Chinese listed enterprises, we study whether the implementation of macroprudential policy leads to enterprise policy arbitrage. Our findings reveal that tightening macroprudential policies significantly contribute to the promotion of shadow banking activities among Chinese enterprises, confirming the existence of policy leakage. When the overall credit is tight, non‐affiliated loans and loans to privately‐owned enterprises increase significantly. Tightening policies also have a more substantial effect on the borrowing cost of non‐affiliated and privately‐owned enterprise borrowers. To enhance the effectiveness of macroprudential policy, our results suggest the necessity of providing appropriate credit supports for small and medium enterprises.
MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis
Authors: Sun Yujia , Huang Yongding , Hao Zhitai , Zhang Shoutao , Tian Qingnan 
Published: June 2023

AbstractAdult stem cells (ASCs) are pluripotent cells with the capacity to self‐renew and constantly replace lost cells due to physiological turnover or injury. Understanding the molecular mechanisms of the precise coordination of stem cell proliferation and proper cell fate decision is important to regeneration and organismal homeostasis. The planarian epidermis provides a highly tractable model to study ASC complex dynamic due to the distinct spatiotemporal differentiation stages during lineage development. Here, we identified the myosin regulatory light chain (MRLC) homologue in the Dugesia japonica transcriptome. We found high expression levels of MRLC in wound region during regeneration and also expressed in late epidermal progenitors as an essential regulator of the lineage from neoblasts to mature epidermal cells. We investigated the function of MRLC using in situ hybridization, real‐time polymerase chain reaction and double fluorescent and uncovered the potential mechanism. Knockdown of MRLC leads to a remarkable increase in cell death, causes severe abnormalities during regeneration and homeostasis and eventually leads to animal death. The global decrease in epidermal cell in MRLC RNAi animals induces accelerated epidermal proliferation and differentiation. Additionally, we find that MRLC is co‐expressed with cdc42 and acts cooperatively to control the epidermal lineage development by affecting cell death. Our results uncover an important role of MRLC, as an inhibitor of apoptosis, involves in epidermal development.
Proposed museum service operations framework for addressing overcrowding: A Taipei case study
Authors: Su Yaohua 
Published: June 2023

AbstractAlthough the high growth of the tourism market contributes to a record number of visitors to landmark museums, they also encounter the overcrowding problem. This study reviewed the case of National Palace Museum (NPM) in Taipei to discuss the overcrowding problem and provide suggestions to alleviate this problem. This study adopted document analysis to summarize NPM's policies regarding the overcrowding problem that were identified by performing text mining of visitors' reviews for the NPM. And a content analysis involving semantic analysis and coding of negative reviews for NPM retrieved from TripAdvisor was performed for extracting complaint categories regarding the overcrowding problem. On the basis of the results, a service operation management framework in response to museum overcrowding is proposed. This study suggests that a museum can manage the overcrowding problem on the basis of visitor characteristics by adjusting visitor demand, optimizing the service design, diverting visitors' flow, elaborating the floor plan and exhibition design, or forming a decentralized museum hub to move must‐see exhibits for tourists to a stand‐alone pavilion or branch to disperse the crowd.
Bladder prolapse with simultaneous entrapment of a portion of the descending colon through the urethral sphincter secondary to dystocia in a mare
Authors: Sikorra Weronika , Berreta Ana , Voermans Margreet , Robinson Claire 
Published: June 2023

SummaryBladder prolapse is rare in mares but may occur as a result of excessive straining during parturition or postpartum. We report a case of dystocia‐associated prolapse and entrapment of the small colon through the external urethral opening into a prolapsed urinary bladder. No previous reports of this condition were found. An 11‐year‐old Thoroughbred mare was presented to The Equine Hospital of the Jockey Club of Saudi Arabia for evaluation and treatment of dystocia. Prolapse of the urinary bladder was concomitantly diagnosed. After initial assessment, replacement of the urinary bladder in the normal position did not appear possible. The mare was immediately prepared for caesarean section. The foal was successfully delivered and resuscitated. Detailed assessment of the bladder during surgery revealed that the small colon had prolapsed through the external urethral opening and was inside the space formed by the everted bladder. The urinary bladder was opened. The small colon was replaced into the abdominal cavity; a surgeon gently replaced the small colon through the external urethral sphincter and a second surgeon retrieved the small colon by gentle traction until normally aligned. The bladder was closed and replaced. The mare recovered uneventfully. The mare and the foal were discharged from the hospital 17 days post‐operatively.
Dupilumab‐associated ocular adverse events are predicted by low tear break‐up time and correlate with high IL‐33 tear concentrations in patients with atopic dermatitis
Authors: Chiricozzi A. , Di Nardo L. , Gori N. , Antonelli F. , Pinto L. , Cuffaro G. , Piro G. , Savino G. , Tortora G. , Peris K. 
Published: June 2023

AbstractDupilumab, blocking IL‐4 and IL‐13 signals, improves atopic dermatitis and Quality of Life but might be also associated with the occurrence of ocular adverse events (OAEs). The main objective of our prospective study was to characterize the cytokine and chemokine profile in the tear fluid of dupilumab‐treated patients with moderate‐to‐ severe atopic dermatitis and to identify biomarkers predicting the occurrence of ocular adverse events. Patients with moderate‐to‐severe AD underwent dermatological and ophthalmological evaluation at the baseline (T0) and week 16 or at the time of an eventual ocular adverse events (T1). A multiplex immunoassay measuring multiple cytokines and chemokines in the tear fluid extracted during ocular examination at both T0 and T1 was performed. Thirty‐nine patients with moderate‐to‐severe AD and treated with dupilumab were included in the study. Baseline tear fluid levels revealed a significantly higher concentration of type 2 cytokines and chemokines in AD patients than healthy controls. The occurrence of ocular adverse events during dupilumab therapy was associated with a significant increase of IL‐33 tear fluid levels and a significantly lower tear break‐up time, this latter also identified as predictive factor. Our findings suggest that the ophthalmological examination should be considered a valid support to identify patients at risk of developing OAEs and to provide their appropriate management.
How do I work this? A mixed‐methods evaluation of relationship education for high school youth
Authors: Bradford Kay , Harris Jennifer , Boehme Raquel , Turner Joshua J. , Higginbotham Brian 
Published: June 2023

AbstractObjectiveGuided by social exchange theory, we used mixed methods to examine youth romantic relationship concerns, perceived gains from a relationship education curriculum, and the extent to which the curriculum addressed adolescents' concerns.BackgroundAdolescents' relationships influence the health of their future romantic relationships. Relationship education is increasingly offered to youth in the United States, but few studies examine how well these programs address youth concerns.MethodsPhenomenological content analysis was used to analyze short‐response qualitative data (n = 267) about romantic relationship concerns (pretest) and programmatic gains (posttest). Themes were then compared to examine how well the curriculum addressed youth concerns. Quantitative pretest–posttest outcomes were also examined among a larger sample (n = 9,130). Complementarity was used to consider qualitative and quantitative findings together.ResultsParticipants' pretest concerns included relationship knowledge (i.e., how, with whom, and when to have a relationship), and relationship risks (e.g., cheating, coercion). Posttest themes suggested that program content largely addressed participants' concerns (i.e., increased knowledge, ability to discern healthy and unhealthy relationships). Quantitative analyses yielded significant changes in four measures of participant attitudes pretest to posttest, with increased relationship knowledge and healthier attitudes.ConclusionsYouth perceived that relationship education gave them tools that addressed their stated concerns; quantitative data suggest the program had its intended effect.ImplicationsYouth relationship education is a positive preventative intervention.
Using multivariate autoregressive state‐space models to examine stock structure of Greenland halibut in the North Atlantic
Authors: Úbeda J. , Nogueira A. , Tolimieri N. , Vihtakari M. , Elvarsson B. , Treble M. , Boje J. 
Published: June 2023

AbstractAccurate information on population structure is essential for effective fisheries management. Greenland halibut (Reinhardtius hippoglossoides) in the North Atlantic is managed as four separate offshore stocks. We use Multivariate Autoregressive State‐Space (MARSS) models to assess population structure by means of abundance and biomass trends in four regions (Norwegian Sea, Iceland, Southeast Greenland, and Northwest Atlantic) where three offshore stocks are recognized: (1) Baffin Bay–Davis Strait (Northwest Atlantic stock), (2) Southeast Greenland and Iceland (West Nordic stock (WNS)), and (3) the Barents and Norwegian Seas (Northeast Arctic stock). We formulated model alternatives, using bottom trawl survey data from each region for 1996–2019, to evaluate support for different population structures. Abundance and biomass observations from each region were linked to growth rate parameters in MARSS models and the impact of climate (North Atlantic Oscillation Index) and fishing (commercial catches) on stock dynamics was investigated. Top models identified the Northwest Atlantic as an independent population. Best‐fit models treated Greenland halibut in the WNS as two independent populations (east and west), with potential connections between eastern Iceland and the western Barents Sea. These results suggest a mismatch between current stock perception and management boundaries in the Northeast Atlantic.
Pyrolysis temperature determines the amendment effects of poplar residue‐derived biochars on reducing CO2 emission
Authors: Ding Sihui , Lan Ziyu , Fang Shengzuo 
Published: June 2023

AbstractPoplars and their hybrids are widely planted in both plantation forestry and agroforestry systems of the world. Along with the utilization and plantation management processes, a large amount of biomass residues are produced, but the relationship between biochar properties and soil CO2 emissions is largely unknown. Here, a laboratory incubation study was conducted to assess the effects of different biochars and their corresponding biomass residues on soil CO2 emissions during the 180 days of incubation. Poplar residue‐derived biochars were larger in the surface area and total pore volume but lower in nutrients and pH values than the rice straw‐derived biochar. Increasing pyrolysis temperature led to a decrease in the total nitrogen (TN) content of poplar leaf‐ and rice straw‐derived biochars, but enhanced the TN in the poplar twig‐ and poplar bark‐derived biochars. After 180‐day incubation, the total cumulative CO2 emission decreased by 33.1%–73.8% in the biochar amendments compared to their corresponding biomass residue addition, whereas the biochars derived from poplar twig and bark residues had more positive effects on reducing soil CO2 emissions, but depended on the pyrolysis temperature. Correlation analysis showed a significant and positive correlation between the CO2 emissions and TN content of bio‐based materials but the negative relationships to total carbon content and C/N ratio. Meanwhile the positive correlations of CO2 emissions to the surface area, t‐plot micropore area, and volume of the biochars were detected. Our results suggest that application of poplar twig‐ and poplar bark‐derived biochars has a great potential for mitigating global warming.
Exploring the molecular characteristics of the malignant potential of gastric adenocarcinoma with enteroblastic differentiation
Authors: Wang Yong , Wei Xiyin , Ke Bin , Liu Jia , Guo Yuhong , Liu Yanxue , Chen Yongzi , Ding Tingting , Wang Yalei , Meng Bin , Sun Baocun , Zang Fenglin 
Published: June 2023

AimsGastric adenocarcinoma with enteroblastic differentiation (GAED) is a rare subset of alpha‐fetoprotein (AFP)‐producing carcinomas with poor prognosis. However, the molecular features associated with the malignant potential of GEAD remain partially elucidated.Methods and resultsIn this study, the relationship between clinicopathological parameters and aggressive biological behaviour was analysed in 37 patients with GAED. The results showed that GAED tended to infiltrate the deep layer of the gastric wall and possessed more frequent vascular invasion than conventional gastric adenocarcinoma (CGA) (P < 0.001). All distant metastases were observed in the GAED group, not the CGA group (P < 0.001). High HER2 expression was found in nearly 24.32% of the informative cases, and none showed EBV‐encoded RNA positivity or deficient mismatch repair. The most frequently mutated gene in GAED was p53. Programmed cell death‐ligand 1 (PD‐L1) immunostaining revealed 13 patients with a combined positive score (CPS) ≥ 5 (65%, 13 of 20). Thus, based on these molecular markers (immunostaining, in situ hybridisation and mutation analysis), GAED may be classified as a unique subgroup of the chromosomal instability subtype with HER2+/EBV−/MSS/TP53+/PD‐L1+. Next‐generation sequencing analyses showed that mutations in the TOPI, ELOA and NOTCH3 genes were found only in GAED, and abnormally expressed genes in GAED were significantly enriched in hepatocellular carcinoma‐, gland development‐, and gastric cancer‐related pathways.ConclusionThe HER2+/EBV−/MSS/TP53+/PD‐L1+ profile and hepatocellular carcinoma‐related pathways may be significant in the malignant potential of GAED. In addition to anti‐HER2 therapy, immune check‐point inhibitors may be an effective treatment option for patients with GAED.