bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of transport on cortisol, glucose, heart rate, leukocytes and body weight in captive-reared guanacos (Lama guanicoe)
Authors: Zapata B , Gimpel J , Bonacic C , González BA , Riveros JL , Ramírez AM , Bas F , Macdonald DW 
Published: January 2023

AbstractCurrent procedures for ranching and sustainable use of guanacos necessitate their transport. Transportation is a risky process for animals, and is a particular concern for wild-caught or semi-domesticated species such as the guanaco — a wild South American camelid species increasingly being established on farms in Chile and Argentina. This study investigated the effect of transport on the physiological and behavioural responses of eight castrated adult male guanacos, transported on a single 2 h journey at a stocking density of 113.5 kg m-2(0.76 m2per animal). Plasma cortisol and blood glucose concentration, total and differential white blood cell (WBC) counts, heart rate, and body weight were measured one week before, immediately before, immediately after, 2 h after and one week after transport. Behavioural responses were recorded during handling prior to loading. Immediately after transport we found significant increases in plasma cortisol concentrations and neutrophil:lymphocyte (N:L) ratio, the latter peaking 2 h after transport. Heart rate increased significantly only during loading, while body weight remained constant throughout. Behavioural responses related to handling (jumping, vocalising, kicking, spitting and urinating) were not associated with the physiological response. All variables returned to pre-transport values within one week. Transport of guanacos under these conditions produced physiological changes similar to those associated with a mild and transient stress response in other species and which, we judge, fall comfortably within acceptable limits for their welfare.
Comparison of the severity of esophagogastric, lung and limb lesions at slaughter in pigs reared under standard and enriched conditions
Authors: Ramis G , Gómez S , Pallarés FJ , Muñoz A 
Published: January 2023

AbstractTwo hundred and ten pigs were reared in three groups (according to genotype) under enriched conditions of large open-front sawdust-bedded barns. Eight hundred and twenty pigs were reared under standard conditions of small 15-animal pens in a conventional barn with partially slatted floors and natural ventilation. Production parameters including percentage mortality, feed conversion rate and average daily weight gain were calculated at the end of the fattening period. Stomachs, limbs and lungs were examined at slaughter in order to compare the number and severity of lesions between the enriched environment groups and the standard environment group. There was a significantly greater number of esophagogastric lesions in the standard environment group than in the enriched environment groups. No esophagogastric ulcers were observed in any pigs from the enriched groups, while 17.5% of stomachs from pigs in the standard environment group displayed this condition. There were no significant differences in the number of lung lesions associated with enzootic pneumonia between pigs from the standard and enriched environment groups. The limbs of animals reared in the standard environment had more lesions, especially in floor-contact areas, than those reared in the enriched environment (23.84% versus 1.08%). The production parameters measured were improved in the pigs from the enriched environment. These results suggest that the welfare of fattening pigs may be improved by the provision of enrichment in their housing environment. Evaluation of esophagogastric ulceration could be a useful indicator of welfare in pigs.
Effect of supplementary ultraviolet lighting on the behaviour and corticosterone levels of Japanese quail chicks
Authors: Smith EL , Greenwood VJ , Goldsmith AR , Cuthill IC 
Published: January 2023

AbstractMost birds have visual sensitivity to ultraviolet (UV) wavelengths, and this sensitivity appears to play a role in their colour vision. Artificial lighting is normally deficient in UV wavelengths. Hence, there may be welfare implications for captive birds kept under such lighting. We investigated whether the absence of UV wavelengths during rearing adversely affects Japanese quail (Coturnix coturnix japonica). We also investigated the short-term effect of switching from UV-containing to UV-deficient lighting, and vice versa. Stress was assessed by monitoring behaviour and plasma corticosterone levels. We did not detect any significant difference in these variables between birds reared either with or without UV. We conclude that rearing quail in an absence of UV does not appear to have a significant impact on their welfare, as measured using these indicators.
Bowhunting deer
Authors: Gregory NG 
Published: January 2023

AbstractThere are conflicting views about the humaneness of the handbow as a recreational hunting method for deer. Some claim that it is the most humane hunting method, whilst others report higher wounding rates and crippling losses than with the rifle. This commentary summarises the factors affecting the likelihood of a quick death, the types of equipment commonly used, the vital target areas, the influence of blood loss on blood pressure and brain function and the prevalence of wounding during routine bowhunting. Some requirements in Bowhunters Association Codes of Conduct are also described. It is concluded that where bowhunting is allowed, Codes of Conduct should emphasise the hunters' responsibility to track and despatch injured animals, and adherence to the Codes should be encouraged, if not enforced.
The influence of visual stimulation on the behaviour of dogs housed in a rescue shelter
Authors: Graham L , Wells DL , Hepper PG 
Published: January 2023

AbstractThis study explored the influence of five types of visual stimulation on the behaviour of 50 dogs housed in a rescue shelter. These conditions were: one control condition (no visual stimulation) and four experimental conditions (blank television screen, and moving televised images of conspecifics, interspecifics [ie unfamiliar animal species] and humans). The dogs were exposed to each condition for 4 h per day for five days, with an intervening period of two days between conditions. The dogs' behaviour was recorded on days 1, 3 and 5 during each condition. Dogs spent relatively little of the total observation time looking at the television monitors (10.8%). They spent significantly more of their time looking at the moving images of conspecifics, interspecifics and humans than at the blank screen, although their interest in all experimental conditions declined over time. Dogs spent more time at the front of their enclosures during all of the experimental conditions than during the control condition. Images of conspecifics were more likely to attract the dogs to the front of their kennels than the blank screen. The conspecific and human conditions of visual stimulation attracted slightly more attention from the dogs than the interspecific condition, although not significantly. All of the experimental conditions encouraged significantly less vocalisation and movement than the control condition. Overall, the findings suggest that the behaviour of kennelled dogs is influenced by visual stimulation in the form of television programmes. Such animals, however, may not benefit from this type of enrichment to the same degree as species with more well-developed visual systems. The addition of other types of enrichment strategy for dogs housed in rescue shelters is advocated.
Nest-building behaviour in male rats from three inbred strains: BN/HsdCpb, BDIX/Or1Ico and LEW/Mol
Authors: Jegstrup IM , Vestergaard R , Vach W , Ritskes-Hoitinga M 
Published: January 2023

AbstractThree inbred strains of male laboratory rat (BN/HsdCpb, BDIX/Or1Ico and LEW/Mol) were provided with nest boxes and nest materials, and were observed for nest-building activity. After 7 days, each cage and nest box was examined. Each nest was weighed and scored for complexity, and returned to the cage. This was repeated after a further 7 days and the nest removed completely. This routine was repeated three times. All three strains of rat built nests inside the nest box and showed the same stages of construction. There was a significant increase in nest complexity between day 7 and day 14 in the strains BN and BDIX. Furthermore, BDIX rats used significantly more material for the nests, compared with the BN and LEW rats. In a second experiment, using the same rats, nest material was offered in four different ways. LEW rats used nesting material irrespective of where it was placed; BN rats only used straw placed on the top of the cage when no alternative was provided in the cage, and would not use the nest box roof when it was covered with bedding; whereas BDIX rats would only use nest material placed within the cage and would not use straw placed on the cage lid. BN rats also used nest material to cover the entrance to the nest box, a practice not carried out by the other strains. This study demonstrates that these rat strains have retained their natural nest-building behaviour. We suggest that the correct stimuli must be provided in order for this behaviour to be exhibited; in addition, the way in which the nest materials are provided must be in accordance with strain-specific characteristics.
The use of conjoint analysis to determine the importance of factors that affect on-farm welfare of the dairy cow
Authors: Angus LJ , Bowen H , Gill LAS , Knowles TG , Butterworth A 
Published: January 2023

AbstractA large range of variables can affect the welfare of the dairy cow, making it difficult to assess the overall ‘level of welfare’ of the individual animal. Two groups of individuals completed a questionnaire based upon the ‘five freedoms’: 26 respondents had expertise either in the field of dairy cow welfare or as practicing veterinary surgeons, and 30 were veterinary students in their penultimate year of study. Conjoint analysis was used to calculate the average importance scores (AIS) for 34 variables presented to the respondents as 52 ‘model cows’ in the form of grouped questions, phrases and pictures. Conjoint analysis identified the most important factors for each ‘freedom’: access to forage, body condition score, foot conformation, hock lesions, and the encouragement required for a dairy cow to walk into the parlour. There was a significant difference between the expert and student groups for seven out of 34 factors, which may be attributed to individual variation of opinion, knowledge, experience and expectation. The factors were ranked within each ‘freedom’ using the experts' AIS but it was not assumed that each freedom had equal ‘weight’; therefore, the factors within each freedom were compared only with factors within the same freedom. These scores produced a weighting scale, which was applied on-farm, in a preliminary exercise comparing ‘model’ and ‘perceived’ welfare scores.
Meta-analysis — a systematic and quantitative review of animal experiments to maximise the information derived
Authors: Phillips CJC 
Published: January 2023

AbstractMeta-analysis provides a tool to statistically aggregate data from existing randomised controlled animal experiments. The results can then be summarised across a range of conditions and an increased pool of experimental data can be subjected to statistical analysis. New information can be derived, but most frequently the results are a refinement of existing knowledge. By designing experiments and reporting protocols, so that they have the capability of being useful to meta-analyses, maximum benefit can be derived from individual randomised controlled experiments, which may individually have little statistical power, and new avenues for productive research identified. The methodology for meta-analysis is derived from clinical trials in the medical sciences. Now that there is substantial output from animal science experiments, there is an opportunity to apply the technique to these and reduce the need for further experimentation. This paper describes the contribution of meta-analysis to the reduction of animals in research and provides details on data collection, analysis, the models used, and on interpreting and reporting the results. Three applications of meta-analysis to the field of animal science are also briefly described. First, the impact of undernutrition on the production and composition of milk from dairy cows confirmed existing knowledge about partitioning scarce nutrients to milk yield and live weight. Second, increased absorption of cadmium — a widespread toxic element — from organic sources was detected in sheep, which was previously untested. Third, no significant relationships were found between common indicators of undernutrition and weight, and condition score in cattle suggesting that the common indicators used are not suitable as evidence of long term undernutrition. This paper concludes that opportunities exist to increase the information gained from animal experiments by subjecting the results to meta-analysis, particularly if this can be anticipated in advance of study protocols being constructed.
The use of databases, information centres and guidelines when planning research that may involve animals
Authors: Smith AJ , Allen T 
Published: January 2023

AbstractIn many countries scientists planning research that may involve the use of animals are required by law to examine the possibilities for replacement, reduction or refinement (the Three Rs) of these experiments. In addition to the large number of literature databases, there are now many specialist databases specifically addressing the Three Rs. Information centres, with a mandate to assist scientists and lay people locate information on the Three Rs, have also been established. Email discussion lists and their archives constitute another, although less quality-controlled, source of information. Furthermore, guidelines for the care and use of animals in research have been produced both by regulatory bodies and scientific organisations. The growth of the internet has put an enormous amount of data into the public domain, and the problems of accessing relevant information are discussed. Suggestions are also given for search strategies when using these information sources.
The effectiveness of environmental enrichment on reducing stereotypic behaviour in two captive vicugna (Vicugna vicugna)
Authors: Parker M , Goodwin D , Redhead E , Mitchell H 
Published: January 2023

AbstractEnvironmental enrichment by increasing foraging behaviour and providing food item choice are widely practised and generally accepted as effective methods for reducing stereotypic behaviour in captive animals. In this study, the effectiveness of increasing foraging patch choice and food item choice on reducing motor stereotypy in two captive vicugna were examined. For the purposes of the study, first, browse was added to the vicugna's enclosure as an additional forage item and, second, the vicugna's normal feed was divided: half being provided in the indoor quarters and half in the outdoor yard. The results revealed that providing browse as an additional forage item increased the observed stereotypic behaviour; however, dividing the vicugna's feed, and therefore increasing forage patch choice, decreased stereotypy. This study was limited because of the small sample size and because the area in which the vicugna were performing stereotypic behaviour was partially visually obscured. However, this study has implications for animal welfare because it highlights the need to evaluate the suitability of foraging enrichment items, and suggests that more research into accommodating the adaptive foraging behaviour of this species in captivity may be necessary.
Animal welfare assurance programs in food production: a framework for assessing the options
Authors: Fraser D 
Published: January 2023

AbstractVarious animal welfare assurance programs are being used to encourage or require the adoption of animal welfare standards in food production, and to assure the public that such standards are followed. The programs involve five main formats. Non-mandatory codes/guidelines are relatively easy to institute and appear well-supported by the industry, but provide only minimal assurance to the public unless measures are taken to ensure compliance. Programs based on government regulations and inter-governmental agreements are more challenging to institute; they are likely to generate less industry acceptance, but may provide more public confidence if enforcement is adequate. Product differentiation programs, and retailer policies requiring products to meet certain standards, serve a range of functions; these may generate public confidence but only for products covered. The various programs include several types of requirements. Requirements that are designed to maintain animal health and functioning have a widely accepted scientific basis, are often easy to incorporate into existing production systems, and often provide economic benefits, but do not fully address public concerns over animal welfare in some cultures. Requirements that address pain, distress and other affective states, and those that accommodate certain natural behaviour, have a growing but less traditional scientific rationale and appear likely to generate public confidence; however, they sometimes require significant changes to existing practices. Requirements for more natural surroundings (outdoor, free-range) seem to generate public confidence, but appear most likely to increase costs, least likely to be supported by the existing industry, and may involve trade-offs with productivity and with other aspects of animal welfare. The various formats and requirements provide a range of policy options for addressing animal welfare concerns in different cultural, industry and market contexts.
A non-intrusive method of assessing plumage condition in commercial flocks of laying hens
Authors: Bright A , Jones TA , Dawkins MS 
Published: January 2023

AbstractStandard techniques for assessing plumage damage to hens from feather pecking typically require capture and handling. Handling of individual birds for plumage assessment is relatively easy in experimental studies; however, close inspection of individual birds in commercial flocks is less feasible because catching birds is difficult, may compromise bird welfare and affect egg production. The aim of this study was to assess a non-intrusive method for scoring plumage damage in a commercial free-ranging flock of laying hens. Plumage damage was scored within a 2 m distance of the birds, without capture or handling, using a 5-point scale for 5 body regions. The feather scores, recorded at a distance, by two independent scorers were compared (distance scores), and were then compared with feather scores recorded by a scorer who caught and handled the birds to examine the plumage damage closely (capture scores). There was a significant and positive correlation between the distance scores and the capture scores, and the mean correlation coefficient for all plumage score traits was 0.89. There was also a significant and positive correlation between scorers, and the mean correlation coefficient for all plumage score traits was 0.84. The standard deviation of the residual mean difference between scorers and between methods was less than 1 point for individual body regions and less than 1.5 points for the total body score. Large variation in feather damage within a flock and small sample size increased the standard error of the mean total feather score. When feather damage variation within flocks is low (ie little observed feather damage), the current industry standard of scoring a sample of 100 birds is likely to provide a reliable estimate of flock feather damage; however, when there is large variation within birds of a flock (ie considerable observed feather damage) ≥200 birds should be inspected to accurately monitor changes in plumage condition. The non-intrusive method of feather scoring described in this paper may be useful for commercial-scale feather pecking studies or for farmers who need to assess the plumage damage of their flocks reliably, quickly and with minimal disturbance or stress to the birds.
Refinement of the use of non-human primates in scientific research. Part III: refinement of procedures
Authors: Rennie AE , Buchanan-Smith HM 
Published: January 2023

AbstractThere is an ethical and scientific need to minimise the harm experienced by animals used in scientific procedures and to maximise their well-being. Welfare can be improved by the refinement of practice, particularly if these refinements are applied to every aspect of the life of an animal used in the laboratory, from birth to death. Primates are considered likely to have a greater capacity for suffering than other sentient species and therefore refinement of their use is particularly important. The refinement of the human impact on laboratory-housed primates and of housing and husbandry practices are dealt with in parts I and II of this three-part review. In part III, methods of refinement that can be applied specifically to the use of primates in procedures, are summarised and discussed, together with a description of some current practices, and the scientific evidence that suggests that they should no longer be used. Methods of refinement of identification, capture and restraint, sampling, administration of substances, humane endpoints, and euthanasia are included. If these methods are used, taking into account species-specific differences and needs, it is concluded that harm can be minimised and primate welfare improved.
Training laboratory-housed non-human primates, part 1: a UK survey
Authors: Prescott MJ , Buchanan-Smith HM 
Published: January 2023

AbstractTraining using positive reinforcement is increasingly recognised as a valuable tool for the humane and effective management and use of laboratory-housed non-human primates. We utilised a mixed-mode questionnaire to survey use of training and other learning processes (socialisation, habituation and desensitisation) in over half of UK establishments using and breeding primates. The survey demonstrated that there is widespread awareness of training as a refinement technique and appreciation of its diverse benefits, but training is not used as widely or as fully as it might be. This is due to real constraints (principally staff and time and a lack of confidence in ability to train), and perceived constraints (such as a supposed lack of published information on how to train and assessment of the benefits, and an overestimation of the time investment needed). There is also considerable variation between establishments in the purposes of training and techniques used, with a reliance on negative reinforcement in some. We conclude that there is opportunity for refinement of common scientific, veterinary and husbandry procedures (such as blood and urine collection, injection, capture from the group and weighing) through use of positive reinforcement training, especially when combined with appropriate socialisation, habituation and desensitisation. We end this paper with recommendations on best practice, training techniques and staff education.