bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of baseline transmitral diastolic mean gradient on left atrial pressure reduction in patients undergoing transcatheter mitral valve edge‐to‐edge repair
Authors: Lugo‐Fagundo Nahyr , Pierre Keniel , Adedinsewo Demilade , Simard Trevor , Alkhouli Mohamad , Eleid Mackram F. , Rihal Charanjit S. , Guerrero Mayra , El Sabbagh Abdallah 
Published: January 2023

AbstractBackgroundElevated transmitral gradient post transcatheter mitral valve edge‐to‐edge repair (TEER) has been associated with worse outcomes. Whether an elevated baseline transmitral diastolic mean gradient (MG) ≥5 mmHg is associated with hemodynamic outcomes after TEER is unknown.MethodsA total of 164 consecutive patients undergoing TEER at Mayo Clinic between June 2014 and May 2018 were analyzed in this retrospective study. Baseline demographics, as well as clinical, echocardiographic, and procedural data were obtained. Data on direct left atrial pressure (LAP) before and after TEER were recorded. Logistic regression models were constructed to evaluate the association between preprocedure transmitral diastolic mean gradient (pre‐MG) and (1) improvement in LAP following TEER, (2) postprocedure transmitral diastolic mean gradient (post‐MG). A decrease in LAP post TEER was considered an improvement in hemodynamic response. Pre‐MG was categorized as: ≥5 and <5 mmHg.ResultsMedian age of the cohort was 81.5 years (Q1: 76.3, Q3: 87) and 34% were female. At baseline, median transmitral diastolic MG was 4 mmHg (Q1: 3, Q3: 5) and median LAP was 19 mmHg (Q1:16, Q3: 23.5). In a multivariable model, adjusted for age and sex, patients with pre‐MG ≥ 5 mmHg were less likely to see an improvement in LAP post TEER (adjusted odds ratio [aOR]: 0.22, 95% confidence interval [CI]: 0.09, 0.55; p = 0.001) and more likely to have elevated post‐MG (aOR; 7.08, 95% CI: 2.93, 17.13; p < 0.001).ConclusionHigher pre‐MG (≥5 mmHg) was associated with a lower reduction in LAP and higher residual transmitral gradient following TEER suggesting other potential contributors to increased LAP besides mitral regurgitation as a cause of elevated baseline MG.
A data‐driven soft sensor based on weighted probabilistic slow feature analysis for nonlinear dynamic chemical processes
Authors: Zhang Miao , Xu Beike , Zhou Le , Zheng Hui , Jie Jing 
Published: January 2023

AbstractModeling high‐dimensional dynamic processes is a challenging task. In this regard, probabilistic slow feature analysis (PSFA) is revealed to be advantageous for dynamic soft sensor modeling, which can extract slowly varying intrinsic features from high‐dimensional data. However, nonlinearities prevalent in industrial processes are not considered, which could lead to unsatisfactory prediction performance. In this paper, a weighted PSFA (WPSFA)‐based soft sensor model is proposed for nonlinear dynamic chemical process. In WPSFA, a weighted log‐likelihood function of complete data is constructed to linearize the nonlinear state emission equation. Then, the expectation maximization algorithm is applied to estimate the model parameters and a locally weighted regression model is established for quality prediction. Finally, the feasibility and effectiveness of the proposed approach are well illustrated through a numerical example and a real industrial process.
AAZTA‐Like Ligands Bearing Phenolate Arms as Efficient Chelators for 68Ga Labelling in vitro and in vivo
Authors: Martinelli Jonathan , Zapelli Leonardo Maria , Boccalon Mariangela , Vágner Adrienn , Nagy Gábor , Fekete Anikó , Szikra Dezső , Trencsényi György , Baranyai Zsolt , Tei Lorenzo 
Published: January 2023

AbstractThe introduction of a phenolate pendant arm in place of an acetate on AAZTA‐ and DATA‐like ligands resulted in hepta‐ and hexadentate chelators able to form Ga(III) complexes with thermodynamic stability and kinetic inertness higher than that of other Ga(III) complexes based on the parent 6‐amino‐6‐methylperhydro‐1,4‐diazepine scaffold. In particular, the heptadentate AAZ3A‐endoHB with a phenolate arm on an endocyclic N‐atom shows a logKGaL of 27.35 and a remarkable resistance to hydroxide coordination up to basic pH (pH>9). This behaviour allows to also improve the kinetic inertness of the complex showing a dissociation half‐life (t1/2) at pH 7.4 of 76 h. Although also the hexadentate AAZ2A‐exoHB chelator forms a stable (logKGaL=24.69) and inert (t1/2=33 h at pH 7.4) Ga(III) complex, the 68Ga labelling showed a better radiochemical yield with AAZ3A‐endoHB, especially at room temperature. Thus, a bifunctional chelator of AAZ3A‐endoHB was synthesized bearing an isothiocyanate group that was conjugated to the N‐terminus of a c(RGD) peptide for integrin receptor targeting. Finally, the conjugate was successfully labelled with 68Ga isotope, and the resulting radiotracer tested for its stability in human serum and then in vivo for targeting B16‐F10 tumours with miniPET imaging.
Regio‐ and Stereoselective Carbon‐Boron Bond Formation via Heterogeneous Palladium‐Catalyzed Hydroboration of Enallenes
Authors: Ramesh Naidu Veluru , Rafi Abdolrahim A. , Tai Cheuk‐Wai , Bäckvall Jan‐E. , Córdova Armando 
Published: January 2023

AbstractA highly efficient regio‐ and stereoselective heterogeneous palladium‐catalyzed hydroboration reaction of enallenes was developed. Nanopalladium immobilized on microcrystalline cellulose (MCC) was successfully employed as an efficient catalyst for the enallene hydroboration reaction. The nanopalladium particles were shown by HAADF‐STEM to have an average size of 2.4 nm. The cellulose‐supported palladium catalyst exhibits high stability and provides vinyl boron products in good to high isolated yields (up to 90 %). The nanopalladium catalyst can be efficiently recycled and it was demonstrated that the catalyst can be used in 7 runs with a maintained high yield (>80 %). The vinylboron compounds prepared from enallenes are important synthetic intermediates that can be used in various organic synthetic transformations.
MicroRNAs affecting the susceptibility of melanoma cells to CD8+ T cell‐mediated cytolysis
Authors: Pane Antonino A. , Kordaß Theresa , Hotz‐Wagenblatt Agnes , Dickes Elke , Kopp‐Schneider Annette , Will Rainer , Seliger Barbara , Osen Wolfram , Eichmüller Stefan B. 
Published: January 2023

AbstractBackgroundThe regulatory functions of microRNAs (miRNAs) in anti‐tumour immunity have been mainly described in immune effector cells. Since little is known about miRNA effects on the susceptibility of target cells during T cell—target cell interaction, this study focused on the identification of miRNAs expressed in tumour cells controlling their susceptibility to CD8+ T cell‐mediated cytotoxicity.MethodsLuciferase expressing B16F10 melanoma (B16F10 Luci+) cells transfected with individual miRNAs covering a comprehensive murine miRNA library were screened for their susceptibility to lysis by an established cytotoxic T lymphocyte (CTL) line (5a, clone Nβ) specific for the melanoma‐associated antigen tyrosinase‐related protein 2. miRNAs with the most pronounced effects on T cell‐mediated lysis were validated and stably expressed in B16F10 cells. In silico analyses identified common targets of miRNA sets determined by the screen, which were further confirmed by small interfering RNA (siRNA)‐mediated silencing experiments modulating immune surveillance. The Ingenuity Pathway Analysis (IPA) software and RNA sequencing (RNA‐seq) data from miRNA‐overexpressing cell lines were applied to investigate the underlying mechanisms. The Cancer Genome Atlas (TCGA)‐derived miRNA sequencing data were used to assess the correlation of miRNA expression with melanoma patients’ survival.ResultsThe miRNA screen resulted in the selection of seven miRNAs enhancing CTL‐mediated melanoma cell killing in vitro. Upon stable overexpression of selected miRNAs, hsa‐miR‐320a‐3p, mmu‐miR‐7037‐5p and mmu‐miR‐666‐3p were determined as most effective in enhancing susceptibility to CTL lysis. In silico analyses and subsequent siRNA‐mediated silencing experiments identified Psmc3 and Ndufa1 as common miRNA targets possibly involved in the functional effects observed. The analyses of RNA‐seq data with IPA showed pathways, networks, biological functions and key molecules potentially involved in the miRNA‐mediated functional effects. Finally, based on TCGA data analysis, a positive correlation of the conserved miRNAs among the panel of the seven identified miRNAs with overall survival of melanoma patients was determined.ConclusionsFor the first time, this study uncovered miRNA species that affect the susceptibility of melanoma cells to T cell‐mediated killing. These miRNAs might represent attractive candidates for novel therapy approaches against melanoma and other tumour entities.
Species traits and observer behaviors that bias data assimilation and how to accommodate them
Authors: Scher C. Lane , Clark James S. 
Published: January 2023

AbstractDatasets that monitor biodiversity capture information differently depending on their design, which influences observer behavior and can lead to biases across observations and species. Combining different datasets can improve our ability to identify and understand threats to biodiversity, but this requires an understanding of the observation bias in each. Two datasets widely used to monitor bird populations exemplify these general concerns: eBird is a citizen science project with high spatiotemporal resolution but variation in distribution, effort, and observers, whereas the Breeding Bird Survey (BBS) is a structured survey of specific locations over time. Analyses using these two datasets can identify contradictory population trends. To understand these discrepancies and facilitate data fusion, we quantify species‐level reporting differences across eBird and the BBS in three regions across the United States by jointly modeling bird abundances using data from both datasets. First, we fit a joint Species Distribution Model that accounts for environmental conditions and effort to identify reporting differences across the datasets. We then examine how these differences in reporting are related to species traits. Finally, we analyze species reported to one dataset but not the other and determine whether traits differ between reported and unreported species. We find that most species are reported more in the BBS than eBird. Specifically, we find that compared to eBird, BBS observers tend to report higher counts of common species and species that are usually detected by sound. We also find that species associated with water are reported less in the BBS. Species typically identified by sound are reported more at sunrise than later in the morning. Our results quantify reporting differences in eBird and the BBS to enhance our understanding of how each captures information and how they should be used. The reporting rates we identify can also be incorporated into observation models through detectability or effort to improve analyses across species and datasets. The method demonstrated here can be used to compare reporting rates across any two or more datasets to examine biases.
DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
Authors: Pautasso Néstor Abel , Poletta Gisela Laura , Paravani Enrique Valentín , Sasal María Carolina , Simoniello María Fernanda 
Published: January 2023

AbstractIn our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receives rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72 h after a probed natural runoff event toward the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT), and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.
Ruthenium Catalysis in Biological Habitats
Authors: Gutiérrez‐González Alejandro , López Fernando , Mascareñas José L. 
Published: January 2023

AbstractRecent years have witnessed a considerable progress in research aimed at merging transition metal catalysis with chemical and cell biology. Therefore, a crescent number of metal‐catalyzed transformations have been shown compatible with biological media and even with living settings. Of the different transition metals used to build these biocompatible catalysts, ruthenium has demonstrated to be particularly powerful, in part because the resulting complexes exhibit a very good balance between reactivity and biological stability. Indeed, ruthenium complexes have demonstrated utility to promote a great variety of reactions in biologically relevant contexts, from deprotection and redox processes to cycloadditions or photocatalytic transformations. Many of these reactions may enable the development of new type of biological tools and pharmacological strategies.
Effect of rural domestic sewage regeneration irrigation on paddy soil properties and water and nitrogen utilization in southern China
Authors: Xiao Menghua , Li Yuanyuan , Zheng Shizong , Wang Lei , Cai Jiafang , Wang Wei 
Published: January 2023

AbstractRural domestic reclaimed water (RDRW) for agricultural irrigation is an effective way to alleviate the contradiction between the supply and demand of water. In this research, four kinds of irrigation water sources (primary and secondary treated water [R1 and R2], purified water [R3] and river water [CK]) and three kinds of water level regulations (low, medium and high water level control [W1, W2 and W3]) were set to study the impact of RDRW irrigation and its reuse on the properties of paddy soil, water and nitrogen utilization and yield of rice through a combination of field experiments and laboratory and numerical analysis methods. The results showed that compared with river water (CK) irrigation, pH (0–40 cm), electrical conductivity (EC) and organic matter (OM) content (60–80 cm) increased, and ammonium‐nitrogen (NH4+‐N) (0–20 cm) and nitrate‐nitrogen (NO3−‐N) (40–60 cm) content accumulated under RDRW irrigation. Under high water level regulation (W3), NH4+‐N and NO3−‐N content increased, showing the opposite changing trend compared with W1 and W2 regulations. With RDRW irrigation, water and nitrogen use efficiency and rice yield were significantly increased. Under RDRW irrigation, irrigation water use efficiency (IWUE), nitrogen use efficiency (NUE) and rice yield were increased by 48%–70%, 10%–22% and 5%–13%, respectively, compared with CK irrigation. In addition, path analysis established by structural equation modelling (SEM) showed that the irrigation water amount (IWA) had a significant positive impact on NUE and NH4+‐N but had a significant negative impact on IWUE and EC. NUE, IWUE, NO3−‐N and EC had a significant positive impact on yield. The path of IWA= > IWUE= > yield showed a complete mediation effect, the path of IWA= > EC= > yield and NUE= > EC= > yield both showed a partial mediation effect, and the effect size was 27.9% and 25.0%, respectively.
The average degree of edge chromatic critical graphs with maximum degree seven
Authors: Cao Yan , Luo Rong , Miao Zhengke , Zhao Yue 
Published: January 2023

AbstractIn this paper, by developing several new adjacency lemmas about a path on four or five vertices, we show that the average degree of 7‐critical graphs is at least 6. It implies Vizing's planar graph conjecture for planar graphs with maximum degree 7 and its extension to graphs embeddable in a surface with nonnegative Euler characteristic due to Sanders and Zhao, and Zhang.
Mapping emerging trends and South–South cooperation in regional knowledge networks: A bibliometric analysis of avian influenza research in Southeast Asia
Authors: Liverani Marco , Song Kayla , Rudge James W. 
Published: January 2023

AbstractThis paper maps emerging trends and South‐South cooperation in regional knowledge networks through a bibliometric analysis of avian influenza research in Southeast Asia, between 2004 and 2019. The findings indicate that a substantial research output involving researchers and organisations in the region was generated. However, wide disparities between countries existed, both in terms of output and participation in the regional network, which was largely driven by non‐regional actors. A more proactive involvement of institutions for regional cooperation such as the Association of Southeast Asian Nations (ASEAN) would increase local ownership, sustainability and redress imbalances in the regional research system.
Elevated oxysterol and N‐palmitoyl‐O‐phosphocholineserine levels in congenital disorders of glycosylation
Authors: Dang Do An N. , Chang Irene J. , Jiang Xutian , Wolfe Lynne A. , Ng Bobby G. , Lam Christina , Schnur Rhonda E. , Allis Katrina , Hansikova Hana , Ondruskova Nina , O'Connor Shawn D. , Sanchez‐Valle Amarilis , Vollo Arve , Wang Raymond Y. , Wolfenson Zoe , Perreault John , Ory Daniel S. , Freeze Hudson H. , Merritt J. Lawrence , Porter Forbes D. 
Published: January 2023

AbstractCongenital disorders of glycosylation (CDG) and Niemann‐Pick type C (NPC) disease are inborn errors of metabolism that can both present with infantile‐onset severe liver disease and other multisystemic manifestations. Plasma bile acid and N‐palmitoyl‐O‐phosphocholineserine (PPCS) are screening biomarkers with proposed improved sensitivity and specificity for NPC. We report an infant with ATP6AP1‐CDG who presented with cholestatic liver failure and elevated plasma oxysterols and bile acid, mimicking NPC clinically and biochemically. On further investigation, PPCS, but not the bile acid derivative N‐(3β,5α,6β‐trihydroxy‐cholan‐24‐oyl) glycine (TCG), were elevated in plasma samples from individuals with ATP6AP1‐, ALG1‐, ALG8‐, and PMM2‐CDG. These findings highlight the importance of keeping CDG within the diagnostic differential when evaluating children with early onset severe liver disease and elevated bile acid or PPCS to prevent delayed diagnosis and treatment.
Gill histology and ultrastructure in Aplysia depilans (Mollusca, Euopisthobranchia)
Authors: Lobo‐da‐Cunha Alexandre , Alves Ângela , Rodrigues Aurora 
Published: January 2023

AbstractThe gill of Aplysia depilans consists of several wedge‐shaped pinnules with a highly folded structure, differing from the typical ctenidial gills of mollusks. Light microscopy and transmission electron microscopy were used to investigate this organ in juveniles and adults. In this species, the gill epithelium comprised ciliated, unciliated, and secretory cells. The ultrastructural analysis suggests other functions for the gill besides respiration. The deep cell membrane invaginations associated with mitochondria in the basal region of epithelium point to a role in ion regulation. Endocytosis and intracellular digestion were other activities detected in epithelial cells. In juveniles, an intranuclear crystalline structure was seen in some ciliated cells. The presence of an intranuclear crystalline structure was frequently associated with chromatin decondensation, swelling of the nuclear envelope and endoplasmic reticulum cisternae, and abundance of Golgi stacks. As these intranuclear inclusions were not found in the gill of the adult specimens, their occurrence in the two juveniles seems likely to be an anomalous condition whose cause cannot be established at the moment. Mucous cells were the most abundant secretory cells in the epithelium, but a few epithelial serous cells were also found. In addition, large protein‐secreting subepithelial cells had the main cell body inserted in the connective tissue and a long thin neck crossing the epithelium. Mucous cells can be considered responsible for the production of the mucus layer that protects the epithelium, but the specific functions of the epithelial and subepithelial protein‐secreting cells remain elusive. Below the epithelium, a layer of connective tissue with muscle cells lined the narrow hemolymph space. The connective tissue included cells with a large amount of rough endoplasmic reticulum cisternae. Bacteria were found on the surface of the gill, and the most abundant had a thin stalk for attachment to the epithelial cells.
Placentation in the Mexican scincid lizard Plestiodon brevirostris (Squamata: Scincidae)
Authors: Vázquez‐García Erwin , Villagrán‐SantaCruz Maricela 
Published: January 2023

AbstractViviparity is the reproductive pattern in which females gestate eggs within their reproductive tract to complete their development and give birth to live offspring. Within extant sauropsids, only the Squamata (e.g., snakes, lizards, and amphisbaenians) evolved viviparity, representing 20% of the existing species. The genus Plestiodon is represented by 43 species and is one of the most widely distributed genera of the Scincidae in Mexico. The goal of this research has been to determine the placental morphology and ontogeny during gestation in the lizard Plestiodon brevirostris. Specimens were dissected to obtain the embryonic chambers and the embryos were categorized to carry out the correlation between the development stage and the placenta development. The embryonic chambers were processed using the conventional histological technique for light microscopy. The identified embryonic stages were 4, 29, 34, 36, and 39. A thin eggshell surrounds the egg in early developmental stages; however, this structure is already absent in the embryonic hemisphere during the developmental stage 29. The results revealed that P. brevirostris is a lecithotrophic species, but a close maternal‐fetal relationship is established by tissue apposition. Ontogenically, the placental types that form in the embryonic hemisphere are the chorioplacenta, choriovitelline placenta, and chorioallantoic placenta; whereas the omphaloplacenta is formed in the abembryonic hemisphere. The structure of the chorioallantoic placenta in P. brevirostris suggests that it may play a role during gas exchange between the mother and the embryo, due to the characteristics of the epithelia that comprise it. The structure of embryonic and maternal epithelia of the omphaloplacenta suggests a role in the absorption of the eggshell during gestation and possibly in the transport or diffusion of some nutrients. In general, it is evident that ontogeny and placental characteristics of P. brevirostris match those of other species of viviparous lecithotrophic scincids.