bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmon‐Enhanced Single Extracellular Vesicle Analysis for Cholangiocarcinoma Diagnosis
Authors: Jeong Mi Ho , Son Taehwang , Tae Yoo Keung , Park Chan Hee , Lee Hee Seung , Chung Moon Jae , Park Jeong Youp , Castro Cesar M. , Weissleder Ralph , Jo Jung Hyun , Bang Seungmin , Im Hyungsoon 
Published: January 2023

AbstractCholangiocarcinoma (CCA) is a fatal disease often detected late in unresectable stages. Currently, there are no effective diagnostic methods or biomarkers to detect CCA early with high confidence. Analysis of tumor‐derived extracellular vesicles (tEVs) harvested from liquid biopsies can provide a new opportunity to achieve this goal. Here, an advanced nanoplasmonic sensing technology is reported, termed FLEX (fluorescence‐amplified extracellular vesicle sensing technology), for sensitive and robust single EV analysis. In the FLEX assay, EVs are captured on a plasmonic gold nanowell surface and immunolabeled for cancer‐associated biomarkers to identify tEVs. The underlying plasmonic gold nanowell structures then amplify EVs’ fluorescence signals, an effective amplification process at the single EV level. The FLEX EV analysis revealed a wide heterogeneity of tEVs and their marker levels. FLEX also detected small tEVs not detected by conventional EV fluorescence imaging due to weak signals. Tumor markers (MUC1, EGFR, and EPCAM) are identified in CCA, and this marker combination is applied to detect tEVs in clinical bile samples. The FLEX assay detected CCA with an area under the curve of 0.93, significantly better than current clinical markers. The sensitive and accurate nanoplasmonic EV sensing technology can aid in early CCA diagnosis.
A Mechanically Reinforced Super Bone Glue Makes a Leap in Hard Tissue Strong Adhesion and Augmented Bone Regeneration
Authors: Hu Shanshan , Wang Shan , He Qingqing , Li Dize , Xin Liangjing , Xu Chuanhang , Zhu Xingyu , Mei Li , Cannon Richard D. , Ji Ping , Tang Han , Chen Tao 
Published: January 2023

AbstractExisting bone tissue engineering strategies aim to achieve minimize surgical trauma, stabilize the injured area, and establish a dynamic osteogenic microenvironment. The cutting‐edge bone glue developed in this study satisfies these criteria. Inspired by the excellent adhesive properties of mussels, herein, a super osteogenic glue (L‐DPZ) that integrates poly(vinyl alcohol), L‐dopa amino acid, and zeolitic imidazolate framework‐8 characterized by catechol–metal coordination is used to successfully adhere to hard tissue with a maximum adhesive strength of 10 MPa, which is much higher than those of commercial and previously reported bone glues. The stable hard tissue adhesion also enables it to adhere strongly to luxated or broken teeth, Bio‐Oss (a typical bone graft material), and splice fragments from comminuted fractures of the rabbit femur. Then, it is testified that the L‐DPZ hydrogels exhibit satisfactory biocompatibility, stable degradability, and osteogenic ability in vitro. Moreover, the ability to anchor Bio‐Oss and sustained osteogenesis of L‐DPZ result in satisfactory healing in calvarial bone defect models in rabbits, as observed by increased bone thickness and the ingrowth of new bone tissue. These results are expected to demonstrate solutions to clinical dilemmas such as comminuted bone fracture fixation, bone defect reconstruction, and teeth dislocation replantation.
An Ionic Liquid Ablation Agent for Local Ablation and Immune Activation in Pancreatic Cancer
Authors: Huang Junming , Wang Meng , Zhang Fu , Shao Shiyi , Yao Zhuo , Zhao Xinyu , Hu Qida , Liang Tingbo 
Published: January 2023

AbstractPancreatic ductal adenocarcinoma rapidly acquires resistance to chemotherapy, remaining a fatal disease. Immunotherapy is one of the breakthroughs in cancer treatment, which includes immune checkpoint inhibitors, chimeric antigen receptor T‐cell immunotherapy, and neoantigen vaccines. However, immunotherapy has not achieved satisfactory results in the treatment of pancreatic cancer. Immunogenic death comprises proinflammatory cell death, which provides a way to enhance tumor immunogenicity and promote an immune response in solid tumors. Herein, an ionic liquid ablation agent (LAA), synthesized from choline and geranic acid, which triggers necrosis‐induced immunotherapy by remodeling an immunosuppressive “cold” tumor to an immune activated “hot” tumor is described. The results indicate that LAA‐treated tumor cells can enhance immunogenicity, inducing dendritic cell maturation, macrophage M1 polarization, and cytotoxic T lymphocyte infiltration. The results of the present study provide a novel strategy for solid tumor immunotherapy.
Actin Filaments Couple the Protrusive Tips to the Nucleus through the I‐BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers
Authors: Mukherjee Apratim , Ron Jonathan Emanuel , Hu Hooi Ting , Nishimura Tamako , Hanawa‐Suetsugu Kyoko , Behkam Bahareh , Mimori‐Kiyosue Yuko , Gov Nir Shachna , Suetsugu Shiro , Nain Amrinder Singh 
Published: January 2023

AbstractThe cell migration cycle, well‐established in 2D, proceeds with forming new protrusive structures at the cell membrane and subsequent redistribution of contractile machinery. Three‐dimensional (3D) environments are complex and composed of 1D fibers, and 1D fibers are shown to recapitulate essential features of 3D migration. However, the establishment of protrusive activity at the cell membrane and contractility in 1D fibrous environments remains partially understood. Here the role of membrane curvature regulator IRSp53 is examined as a coupler between actin filaments and plasma membrane during cell migration on single, suspended 1D fibers. IRSp53 depletion reduced cell‐length spanning actin stress fibers that originate from the cell periphery, protrusive activity, and contractility, leading to uncoupling of the nucleus from cellular movements. A theoretical model capable of predicting the observed transition of IRSp53‐depleted cells from rapid stick‐slip migration to smooth and slower migration due to reduced actin polymerization at the cell edges is developed, which is verified by direct measurements of retrograde actin flow using speckle microscopy. Overall, it is found that IRSp53 mediates actin recruitment at the cellular tips leading to the establishment of cell‐length spanning fibers, thus demonstrating a unique role of IRSp53 in controlling cell migration in 3D.
Ultrahigh Energy Storage Density in Poly(vinylidene fluoride)‐Based Composite Dielectrics via Constructing the Electric Potential Well
Authors: Zhu Tongguang , Zhao Hang , Zhang Na , Zhang Chuying , Yin Lei , Dang Zhi‐Min , Bai Jinbo 
Published: January 2023

AbstractDielectric capacitors are fundamental energy storage components in electronics and electric power systems due to their unique ultrahigh power density. However, their relatively low energy storage density is a long‐standing challenge which greatly limits their practical application range. Chitosan (CS) and montmorillonite (MMT) are two kinds of materials that exist abundantly on the earth with natural surface charges. The positively charged CS and negatively charged MMT can be self‐assembled into the typical sandwich‐structured CS/MMT/CS 2D structure through an electrostatic attraction. Loading these surface‐charged sandwich‐structured nanosheets into poly(vinylidene fluoride)‐based composite with a weight fraction as tiny as 0.3 wt.%, an ultrahigh energy storage density of 32.5 J cm−3 accompanied with a high efficiency of 64% are concurrently achieved with a very low cost and scalable process. Guided by finite element simulation, it is revealed that a number of electric potential wells that exist in the charged sandwich nanosheets impede the acceleration of internal charges and hinder the growth of electrical trees. The results offer a novel paradigm for exploring ultrahigh energy storage density capacitors in an economical way.
A simple genetic stratification method for lower cost, more expedient clinical trials in early Alzheimer's disease: A preliminary study of tau PET and cognitive outcomes
Authors: Wang Xin , Broce Iris , Qiu Yuqi , Deters Kacie D. , Fan Chun Chieh , Dale Anders M. , Edland Steven D. , Banks Sarah J. ,   for the Alzheimer's Disease Neuroimaging Initiative
Published: January 2023

AbstractIntroductionIdentifying individuals who are most likely to accumulate tau and exhibit cognitive decline is critical for Alzheimer's disease (AD) clinical trials.MethodsParticipants (N = 235) who were cognitively normal or with mild cognitive impairment from the Alzheimer's Disease Neuroimaging Initiative were stratified by a cutoff on the polygenic hazard score (PHS) at 65th percentile (above as high‐risk group and below as low‐risk group). We evaluated the associations between the PHS risk groups and tau positron emission tomography and cognitive decline, respectively. Power analyses estimated the sample size needed for clinical trials to detect differences in tau accumulation or cognitive change.ResultsThe high‐risk group showed faster tau accumulation and cognitive decline. Clinical trials using the high‐risk group would require a fraction of the sample size as trials without this inclusion criterion.DiscussionIncorporating a PHS inclusion criterion represents a low‐cost and accessible way to identify potential participants for AD clinical trials.
A Novel Aluminum‐Based Metal‐Organic Framework with Uniform Micropores for Trace BTEX Adsorption
Authors: Hu Laigang , Wu Wenhao , Gong Li , Zhu Hongxia , Jiang Ling , Hu Min , Lin Daohui , Yang Kun 
Published: January 2023

AbstractMetal‐organic frameworks (MOFs) are potential porous adsorbents for benzene, toluene, ethylbenzene and xylene (BTEX). A novel MOF, using low toxic aluminum (Al) as the metal, named as ZJU‐620(Al), with uniform micropore size of 8.37±0.73 Å and specific surface area of 1347 m2 g−1, was synthesized. It is constructed by one‐dimensional rod‐shaped AlO6 clusters, formate ligands and 4,4′,4′′‐(2,4,6‐trimethylbenzene‐1,3,5‐triyl) tribenzoic ligands. ZJU‐620(Al) exhibits excellent chemical‐thermal stability and adsorption for trace BTEX, e.g., benzene adsorption of 3.80 mmol g−1 at P/P0=0.01 and 298 K, which is the largest one reported. Using Grand Canonical Monte Carlo simulations and Single‐crystal X‐ray diffraction analyses, it was observed that the excellent adsorption could be attributed to the high affinity of BTEX molecules in ZJU‐620(Al) micropores because the kinetic diameters of BTEX are close up to the pore size of ZJU‐620(Al).
What is ESG? Rethinking the “E” pillar
Authors: Trahan Ryan Thomas , Jantz Brad 
Published: January 2023

AbstractEnvironmental, social and governance (ESG) investing suffers from a well‐documented problem of defining exactly what it is. Anecdotal evidence indicates that overinclusion is the more serious immediate problem, as assets stated to be managed in accordance with ESG criteria have undergone broad expansion. Yet, persistent criticism and frustration with the uncertainty of the meaning and aims of ESG present myriad risks. An abrupt outflow of capital, together with attendant environmental impacts, is one potential consequence if the music stops. We offer a corrective supported by empirical research on the energy transition. Our suggestion is that the environmental pillar, the “E” in ESG, is uniquely quantifiable, yet theoretically underdeveloped in important respects. This circumstance corresponds with emissions ratings regimes that are both loose and misleadingly precise. We suggest a five‐point rubric for rethinking the “E” pillar in view of changing socio‐technical systems that result in the secondary effects of emissions.
Drivers to mitigate climate change in context of manufacturing industry: An emerging economy study
Authors: Dwivedi Ashish , Sassanelli Claudio , Agrawal Dindayal , Moktadir Md. Abdul , D'Adamo Idiano 
Published: January 2023

AbstractBusiness strategy and the environment are becoming increasingly central within the global agenda. In particular, some emerging countries such as India show strong growth in the manufacturing sector while also exhibiting high emission values. This inevitably creates a paradox. Therefore, this study aims to determine the drivers for Indian manufacturing firms to pursue sustainability. The methodologies used in this work is comprised of modified total interpretive structure modeling (m‐TISM) that is based on 21 drivers to mitigate climate change and cross‐impact matrix multiplication applied to classification (MICMAC) that evaluates the dependence power and driving power of the drivers. The findings from the study reflect that there are nine levels into which the clusters are divided. “greenhouse gas performance” and “encouraging low carbon products” are influenced by all other drivers, while the driver “dynamic transitions in economic and political situation” occupies a mirror position. This last driver has the greatest impact on the achievement of the goal along with “persistent with corporate conventionalities” and “dynamic transitions in economic and political situation.” The economic dimension has a key asset in the manufacturing context, but the influence of sustainable technology on the supply side and of the responsible consumption on the demand side cannot be overlooked. Businesses that focus on short‐term maximization by not focusing on renewable and circular resources may be uncompetitive in the long‐term.
Integrated evaluation the antioxidant activity of epicatechin from cell dynamics
Authors: Qing Yinglu , Xiang Xiong , Li Shan , Wang Min , Liang Zhengyang , Ren Jiaoyan 
Published: January 2023

AbstractOxidative damage has been implicated in the pathogenesis of numerous disorders by affecting the normal functions of several tissues. Further, oxidative stress acts within cells to influence cell morphology and the behavior of cell migration. The movement and migration of cells are crucial during the development of organisms as they transition from embryo to adult, and for the homeostasis of adult tissues. Epicatechin (EC) is a natural flavonoid derived mostly from tea, chocolate, and red wine. We investigated the protective impact of EC on D‐galactose(D‐gal)/rotenone‐injured NIH3T3 cells and found alterations in cell dynamics throughout the procedure. The results reveal that D‐gal/rotenone stimulation can cause the cell area to expand and the number of cellular protrusions to increase. EC intervention can considerably minimize the oxidative damage of rotenone on NIH3T3 cells (p < 0.05) but showed little influence on cell damage induced by D‐gal. Furthermore, the corrective ability of EC as an antioxidant is reflected in a dose‐dependent effect on cell movement, including variations in movement speed and distance. Overall, from the perspective of cell morphology and cell motility, EC has a good protective impact on cells harmed by rotenone induced oxidative damage, as well as corrective properties as an antioxidant to balance intracellular oxidative stress, which allowing for a more comprehensive evaluation of antioxidant performance of EC.
In situ shell technique for edentulous ridge augmentation
Authors: Houshmand Behzad , Talebi Ardakani Mohammadreza , Amirinasab Omid , Amid Reza , Moscowchi Anahita , Esfahrood Zeinab Rezaei , Ekhlasmand Kermani Mehdi 
Published: January 2023

AbstractBackgroundThe novel two‐stage technique presented in this study is based on guided bone regeneration for three‐dimensional bone augmentation.Methods and ResultsThe proposed technique was performed to augment an atrophic alveolar ridge in the maxilla and mandible. This method is based on using an autogenous bone plate, a mixture of allogeneic bone graft and injectable –platelet‐rich fibrin, and a bioresorbable barrier membrane. Based on the cases presented in this study, sufficient osseous regeneration was achieved to place dental implants in an ideal position.ConclusionWithin the limitations of the present study, it seems that in situ shell technique could be a beneficial method to augment the extremely atrophied ridges with less morbidity and shorter operative time.Key pointsWhy is this case new information?

The cases presented a new technique using in situ autogenous plates for ridge augmentation.
What are the keys to the successful management of this case?

The keys to the successful management of these cases are proper flap management and less traumatic bony plate preparation.
What are the primary limitations to success in this case?

The primary limitation to success in this technique would be a need for high surgical skills to conduct the procedure accurately.
High glucose promotes podocyte movement: From the perspective of single cell motility assay
Authors: Zhang Junhui , Zhang Yuping , Zhang Qiong , Feng Yanhai , Deng Xiuyuan , Deng Fang , Chen Bing , Hu Jiongyu 
Published: January 2023

AbstractPodocytes are highly specialized glomerular epithelial cells that play a crucial role in maintaining the glomerular filtration barrier, impairment of which usually leads to proteinuria. The phenotypic alterations of podocytes are described to be one of the critical mechnisms underlying podocyte detachment from the glomerular basement membrane. High glucose is the major factor mediating the renal damages and podocyte injuries in the process of diabetic nephropathy. It was revealed that high glucose stimulated the epithelial‐to‐mesenchymal transition of podocyte, thus contributing to proteinuria. When the podocytes converse from epithelial phenotype to mesenchymal phenotype, their migratory capacity significantly increases. Previously, cell migration is conventionally detected by the wound healing assay and the transwell assay. In this study, we investigated and comfirmed the possibility of using single cell motility assay for the anaysis of podocyte motility under high glucose condtition.
Transcatheter aortic valve replacement‐related aortic dissection: A clinical case series
Authors: Hiruma Takashi , Higuchi Ryosuke , Saji Mike , Takamisawa Itaru , Shimokawa Tomoki , Nanasato Mamoru 
Published: January 2023

AbstractObjectivesWe review our experience with 13 periprocedural aortic dissection (AD) cases caused by transcatheter aortic valve replacement (TAVR).BackgroundAD is a potentially lethal complication of TAVR; however, only sporadic case reports have been published to date.MethodsAmong 1335 consecutive patients who underwent TAVR in 2013–2021, we retrospectively extracted 13 patients (1.0%) with TAVR‐related AD (Stanford type A in 6 [46%], type B in 7 [54%]). AD was defined as a new‐onset dissected layer of the aortic wall and diagnosed by aortography, computed tomography, or transesophageal echocardiography.ResultsFive of the six type A AD cases (83%) were detected during TAVR versus only one of the seven type B AD cases (14%). Four of the seven type B AD cases (57%) were asymptomatic and incidentally detected on computed tomography. The presumed causes of AD were injury by the delivery sheath (39%), delivery catheter (23%), valve implantation (15%), stent edge (15%), and pre‐dilation balloon (8%). Complicated AD occurred in only one patient (8%). Considering the patient's age and prohibitive surgical risk, all patients were treated conservatively and free of any aortic‐related deaths or interventions during a follow‐up of 1087 days.ConclusionsTAVR‐related AD is a rare but life‐threatening condition that may be underdiagnosed. Its optimal therapy remains unclear, and conservative management might be an option for selected patients. Further studies are needed to elucidate the incidence, risk factors, effective screening, optimal therapy, and outcomes of TAVR‐related AD.
Methanation of CO2Over Nanostructured Cobalt‐Actinide Bimetallic Oxides
Authors: Ferreira Ana C. , Branco Joaquim B. , Martinho Joana F. , Ferraria Ana M. , Botelho de Rego Ana M. , Ferreira Machado I. , Vieira Ferreira L. F. 
Published: January 2023

AbstractNanostructured Cobalt‐Actinide (An=Th, U) bimetallic oxides were for the first time prepared by the epoxide addition method and electrospinning technique aiming the synthesis of aerogels and nanofibers, respectively. Tested as catalysts for the methanation of CO2and regardless of the actinide element, the yield and selectivity towards CH4are high (>40 and 95 %, respectively), which was explained by their physicochemical properties such as reducibility and basicity and the existence of a synergic effect between cobalt and actinide species. The aerogels present the best catalytic behavior, namely those with thorium and the catalytic activity increases with the Co/An molar ratio. Both aerogels and nanofibers also present a high resistance to deactivation for at least 75 h in the gaseous stream, which is an advantage for any catalytic application. Moreover, at 300 °C they are 2 to 4 times more active than two reference catalysts (5wt %Rh/Al2O3and NiO/Al2O3) tested in the same conditions, which to our best knowledge is a significant result.
Diquat Based Dyes: A New Class of Photoredox Catalysts and Their Use in Aerobic Thiocyanation
Authors: Meier Armin , Badalov Sabuhi V. , Biktagirov Timur , Schmidt Wolf Gero , Wilhelm René 
Published: January 2023

AbstractA series of new organic donor–π–acceptor dyes incorporating a diquat moiety as a novel electron‐acceptor unit have been synthesized and characterized. The analytical data were supported by DFT calculations. These dyes were explored in the aerobic thiocyanation of indoles and pyrroles. Here they showed a high photocatalytic activity under visible light, giving isolated yields of up to 97 %. In addition, the photocatalytic activity of standalone diquat and methyl viologen through formation of an electron donor acceptor complex is presented.
Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme
Authors: Chaturvedi Shobhit S. , Thomas Midhun George , Rifayee Simahudeen Bathir Jaber Sathik , White Walter , Wildey Jon , Warner Cait , Schofield Christopher J. , Hu Jian , Hausinger Robert P. , Karabencheva‐Christova Tatayana G. , Christov Christo Z. 
Published: January 2023

AbstractThis study investigates dioxygen binding and 2‐oxoglutarate (2OG) coordination by two model non‐heme FeII/2OG enzymes: a class 7 histone demethylase (PHF8) that catalyzes the hydroxylation of its H3K9me2 histone substrate leading to demethylation reactivity and the ethylene‐forming enzyme (EFE), which catalyzes two competing reactions of ethylene generation and substrate l‐Arg hydroxylation. Although both enzymes initially bind 2OG by using an off‐line 2OG coordination mode, in PHF8, the substrate oxidation requires a transition to an in‐line mode, whereas EFE is catalytically productive for ethylene production from 2OG in the off‐line mode. We used classical molecular dynamics (MD), quantum mechanics/molecular mechanics (QM/MM) MD and QM/MM metadynamics (QM/MM‐MetD) simulations to reveal that it is the dioxygen binding process and, ultimately, the protein environment that control the formation of the in‐line FeIII‐OO⋅− intermediate in PHF8 and the off‐line FeIII‐OO⋅− intermediate in EFE.