bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between mRNA 133b and Patients with Coronary Artery Ectasia and Acute Coronary Syndrome
Authors: Vega-Gomez Jorge Alberto , Ríos-Arce Luis Fernando De los , Sánchez-Muñoz Fausto , Ruiz-Beltrán Arturo Maximiliano , Belderrain-Morales Nayeli , Sarabia-Chao Vianney , Peña-Peña Mario , Jacobo-Albavera Leonor , Mariana Robles-Ledesma , Najera-Rojas Nitzha Andrea , Alonso-Bringas Alma Paola , Sanchez-Leony Giovanna , Gonzalez-Salido Jimena , Gopar-Nieto Rodrigo , Martinez Daniel Sierra-Lara , Gonzalez-Pacheco Hector , Garaygordobil Diego Araiza , Arias-Mendoza Alexandra 
Published: May 2023

Abstract
Background
Coronary artery ectasia (CAE) is an abnormal coronary dilatation that exceeds 1.5x the size of the adjacent coronary normal segments, considered a manifestation of atherosclerosis. However, information regarding its etiology and pathophysiology is scarce. MicroRNAs are small circulating proteins that regulate diverse biological processes and may be involved in tissue development, homeostasis, and cellular communication. We aimed to explore a signature of microRNAs in patients with acute coronary syndrome (ACS) and CAE.
Methods
a prospective case & control study including consecutive patients with ACS (STEMI and NSTEMI) with and without CAE. Plasma samples were obtained, and consequently, quantification of plasma levels of miR-208, miR-208b, miR-1, miR-133b, miR-21, miR-155, miR-126, and miR-16 was performed by qRT-PCR. For analytical purposes, patients were divided into two groups: patients with ACS and CAE and patients with ACS and obstructive CAD. We included 47 patients, 24 with CAE and 23 with obstructive coronary disease.
Results
Patients with CAE showed a higher incidence of obesity (50 vs 21.7%, p = 0.04) and a higher left ventricular ejection fraction (52 vs 41%, p = 0.01). The rest of the baseline clinical characteristics were well-balanced. Among the selected signatures of microRNAs, patients with ACS and CAE showed higher levels of miR-133b than those without CAE. All other analyzed miRNAs were similar among groups.
Conclusions
In patients with ACS and CAE, we found higher miR-133b. Future studies are required to expand the findings of this research work and propose using miR-133b.
Trial Registration
Protocol received approval by the institutional ethics and research committee with the number 21-1248.
Adaptable bio-based dynamically cross-linked networks via disrupting physical cross-linking and chain entanglement for electromagnetic interference shielding materials
Authors: Hu Yun , Chen Lei , Jia Puyou , Ma Yufeng , Huang Qin , Hu Lihong , Zhou Yonghong 
Published: May 2023

AbstractDynamically cross-linked networks combine the uniqueness of thermoplastics and thermosets to allow for reprocessability while being covalently crosslinked, but the lack of functionality seriously limits practical applications. Here, we propose a strategy to develop tung oil based dynamically cross-linked networks (PNMETs) by introducing primary amine to disrupt the physical cross-linking and chain entanglements, and achieved tunable mechanical strength and toughness, chemical stability, self-healing, solid state plasticity, and topological transformation. The constructed PNMETs based on hydrogen bonds and the dynamic imine bond exhibited multiple stimulus responses for light, heat, microwave and infrared radiation, and achieved excellent recycling and self-healing without any catalyst. By doping multiwalled carbon nanotubes (MWCNTs) and nano Fe3O4in PNMETs, the resulted electromagnetic interference (EMI) shielding materials (PNMETs/MWCNT@Fe3O4) were fabricated and realized dual characteristics of dynamically cross-linked networks and EMI shielding material for the first time. PNMETs/MWCNT@Fe3O4composite based on the topological rearrangement of PNMETs showed shape memory behavior, reprocessing, recycling and self-healing property under the conditions of infrared radiation and voltage, as well as exhibited EMI shielding effectiveness of 20–23 dB in the X-band with thickness less than 1 mm, meeting the standards for commercial applications. This work provides a simple but highly practical strategy for the fabrication of functional materials that integrated with dynamically cross-linked polymer and EMI shielding performance suitable for harsh environments.
Synthesis and Characterization of nano γ-Alumina/β -Cyclodextrin as Adsorbent for Heavy Metal Adsorption
Authors: Leila Esfanjani. , Nazanin Farhadyar , Shahbazi Hamid Reza , Fathi Fereshteh 
Published: May 2023

Abstract
Nano γ-alumina (γ-Al2O3) is widely used to adsorb heavy metals due to excellent properties such as high surface area, thermal stability, and high adsorption capacity. Due to poor adsorption of toxic metals on nano γ-Al2O3 surface, coating with β-cyclodextrin improves its adsorption efficiency. The aim of this study was to synthesize nano γ-Al2O3/β-cyclodextrin by co-precipitation method to be used for future study in Cd(II) removal. The Fourier Transmission Infrared (FT-IR) spectroscopy, X- ray powder Diffraction (XRD) and, Scanning Electron Microscope (SEM) were evaluated. The results of XRD and SEM showed nano γ-Al2O3/β-cyclodextrin with 20–30 nm. Also, synthesized nano γ-Al2O3/β-cyclodextrin had suitable structure for cadmium removal as water toxic metal.
Staying on the current niche: Consensus model reveals the habitat loss of a critically endangered dragonfly Libellula angelina under climate changes
Authors: Liu Tong , Liu Haoyu , Li Yuntao , Yang Yuxia 
Published: May 2023

Abstract

Climate change is expected to exert a large impact on the spatial distribution of insects, yet limited analyses are available for assessing the influences of climate change on the distribution of Libellula angelina (Odonata: Libellulidae), which hindered the development of conservation strategies for this critically threatened dragonfly species. Here, a consensus model (BIOCLIM, GAM, MaxEnt and Random Forest) and niche analysis approach were applied to predict the dynamic change of potential distribution areas and ecological niche for L. angelina under future climate change. Meanwhile, the important environmental variables affecting the potential distribution of L. angelina were identified. The results demonstrated that the potential distribution pattern and ecological niche of L. angelina will not shift significantly in face of future climate change, but its highly suitable area in southern Beijing (China), the western and southern South Korea, and the southern Honshu Island (Japan) will decrease constantly. Further analyses indicated that the human influence index (32.3% of variation) is the second highest factor in predicting the potential distribution of L. angelina, following the precipitation of warmest quarter (42.6% of variation). Based on the obtained results, we suggest that extensive cooperation among the countries (China, South Korea and Japan) be advocated to formulate the international conservation strategies, especially more attention and conservation efforts should be paid in those high-suitability areas of L. angelina to gain better protection efficiency, and proper artificial ecological restoration measures should also be exerted. Implication for insect conservation: Our results show that although the critically endangered dragonfly L. angelina will still stay the current niche under climate change, its suitable area (especially highly suitable area) will decrease significantly. Therefore, we suggest that more attention and conservation efforts should be implemented in those high-suitability areas to reduce the extinction risk of L. angelina.
Educational methods used to teach infection prevention and control in nurse training: survey of French training institutes
Authors: Mohammedi Stephanie Bouget , Dinh Claire , Landelle Caroline 
Published: May 2023

Abstract
Context: French nursing students attend classes in Infectiology and Infection Prevention and Control (IPC) during their first semester of training.
Objectives: The aim of this study was to describe the educational techniques used in nurse training to teach IPC and evaluate which factors contribute towards successful exams.
Method: A study was conducted in October 2021 on 320 nurse training institutes in France. An online questionnaire was developed. Factors predicting student success during exams were researched.
Results: In total, 169 institutes took part in the survey, representing 52.8% of all institutes. In these establishments, 91.73% of students passed their IPC examination and the average score was 12.32 out of 20. The exam success rate was significantly linked to the North-West region (Odds Ratio (OR)=1.060, Confidence Interval 95% [CI95%, 1.006-1.117]) and to a number of students higher than 93 (OR=1.034, CI95% [1.004-1.065]) adjusted to the number of educational techniques used. The educational methods applied were varied. There were significant differences in the number of lectures attended and the assessment methods used.
Conclusion: The exam success rate was highly satisfactory but the average score achieved on the exam was not very high.
Assessment of the geotechnical properties and suitability of borrow pit soil for construction works in some Niger Delta communities in Nigeria
Authors: Owamah Hilary Ijeoma , Umukoro Lucky Osiwime , Atikpo Eguakhide 
Published: May 2023

Abstract

Detailed investigation of the geotechnical properties of borrow pits soils, used for road and other construction works in major Niger Delta communities of Nigeria was carried out. The particle size distribution, specific gravity, liquid limit (LL), plastic limit (PL), plasticity index (PI), maximum dry density (MDD), optimal moisture content (OMC), free swelling index (FSI) and Californian bearing ratio (CBR) of the sampled soil were measured in accordance with the British Standard (BS) code and America Standard Testing Material (ASTM) code. Based on the Unified Soil Classification System (USCS), the borrow pits soil can be classified as poorly graded sand with clay. Also, the results depicted that the borrow pits soil Specific gravity, LL, PI values and PL valued ranged from 2.6-2.71, 21.1% -34.08%, 6.29%-13.58% and 15.81% - 20.23%, respectively. OMC, MDD and CBR ranged from 14.53%-15.17%, 1.53 kg/m3 -1.69 kg/m3 and 20.19% -47.15%, respectively. The study affirmed that some of the soils can be useful for the construction of roads and other civil engineering structures, without stabilization.
Intergenic transcription in in vivo developed bovine oocytes and pre-implantation embryos
Authors: Ranjitkar Saurav , Shiri Mohammad , Sun Jiangwen , Tian Xiuchun 
Published: May 2023

Abstract
Background Intergenic transcription, either failure to terminate at the transcription end site (TES), or transcription initiation at other intergenic regions, is present in cultured cells and enhanced in the presence of stressors such as viral infection. Transcription termination failure has not been characterized in natural biological samples such as pre-implantation embryos which express more than 10,000 genes and undergo drastic changes in DNA methylation.Results Using Automatic Readthrough Transcription Detection (ARTDeco) and data of in vivo developed bovine oocytes and embryos, we found abundant intergenic transcripts that we termed as read-outs (transcribed from 5 to 15 kb after TES) and read-ins (transcribed 1 kb up-stream of reference genes, extending up to 15 kb up-stream). Read-throughs (continued transcription from TES of expressed reference genes, 4–15 kb in length), however, were much fewer. For example, the numbers of read-outs and read-ins ranged from 3,084 to 6,565 or 33.36–66.67% of expressed reference genes at different stages of embryo development. The less copious read-throughs were at an average of 10% and significantly correlated with reference gene expression (P < 0.05). Interestingly, intergenic transcription did not seem to be random because many intergenic transcripts (1,504 read-outs, 1,045 read-ins, and 1,021 read-throughs) were associated with common reference genes across all stages of pre-implantation development. Their expression also seemed to be regulated by developmental stages because many were differentially expressed (log2 fold change ≥ 2, P < 0.05). Additionally, while gradual but un-patterned decreases in DNA methylation densities 10 kb both up- and down-stream of the intergenic transcribed regions were observed, the correlation between intergenic transcription and DNA methylation was insignificant. Finally, transcription factor binding motifs and polyadenylation signals were found in 27.2% and 12.15% of intergenic transcripts, respectively, suggesting considerable novel transcription initiation and RNA processing.Conclusion In summary, in vivo developed oocytes and pre-implantation embryos express large numbers of intergenic transcripts, which are not related to the overall DNA methylation profiles either up- or down-stream.
Altered brain tissue composition and microstructure during migraine attacks
Authors: Birkl Christoph , Filippi Vera , Steiger Ruth , Frank Florian , Mangesius Stephanie , Gizewski Elke R. , Broessner Gregor 
Published: May 2023

AbstractDetailed pathophysiological mechanisms causing migraine are still elusive, however, there is evidence that iron metabolization might play a role. Studies using R2 (=1/T2) or R2* (=1/T2*) relaxometry, a common magnetic resonance imaging (MRI) based iron mapping technique, reported increased R2 and R2* values in various brain structures of patients with migraine, indicating an iron accumulation compared to healthy controls. The aim of this first-of-its-kind study was to investigate if there are alterations in R2* in the brain caused by the migraine cycle. Therefore, quantitative MRI, including R2* relaxometry and diffusion tensor imaging (DTI) of a patient with migraine was acquired on 21 consecutive days, comprising headache free days and days with a migraine attack. Combining R2* with DTI, allowed to separate isotropic and anisotropic R2* contributions in white matter. A significant difference in R2* between left and right hemisphere was found in the nucleus accumbens (p = 0.02), the amygdala (p < 0.001) and the pallidum (p < 0.001). During a migraine attack, R2* decreased in the amygdala of the left hemisphere by 13.9% (p = 0.01) and in the nucleus accumbens of the right hemisphere by 7.8% (p = 0.01). In contrast, R2* increased in the caudate of the left hemisphere by 5.3% (p = 0.01). In white matter, isotropic R2* increased by approximately 1.9% (p = 0.04) and anisotropic R2* decreased by approximately 30% (p = 0.02) during a migraine attack. Our study revealed a decrease and increase in iron content in various brain regions during the migraine cycle. Furthermore, white mater iron content increased accompanied by a decrease in anisotropic tissue components during a migraine attack. The observed alterations in R2* indicate that the iron content fluctuates over time depending on the migraine phase. However, the altered iron content reaches normal levels after the migraine attack resolved.
Upregulation of C1QTNF6 mediated by ncRNAs correlates with poor prognosis and immune cells infiltration of clear cell renal cell carcinoma
Authors: Dong wenjing , Feng Shengxing , Zhou Dongsheng , Li Yueming , Qiu Shaopeng , Lin Tianxin , Luo Jinquan , Yuan Runqiang , Gong Mancheng 
Published: May 2023

Abstract
Background
C1q/tumor necrosis factor (C1QTNF) superfamily plays an important role in carcinogenesis of various human cancer types. However, its potential molecular mechanisms for genesis and development of clear cell renal cell carcinoma (ccRCC) remind unclear.
Methods
In our research, pan-cancer analysis for C1QTNF6’s expression and prognosis in patients with ccRCC were conducted by using TCGA data and GTEx data. Target gene prediction tools and StarBase were applied to predict the regulatory pathway of C1QTNF6 in ccRCC. Using TIMER website, we analyzed the the relationships between C1QTNF6 expression level and tumor microenvironment in ccRCC.
Results
Our study revealed that C1QTNF6 might be a carcinogenic gene in ccRCC. Moreover, through correlation analysis, expression analysis and survival analysis, the most potential upstream regulatory pathway of C1QTNF6 in ccRCC, named LINC01694/hsa-miR-10a-5p/C1QTNF6 axis, was established. In addition, our results suggested that the relationships between C1QTNF6 expression level and tumor immune cells infiltration, biomarkers of immune cells and immune checkpoints expression of CTLA-4 and PD1 were significantly positive in ccRCC.
Conclusions
High expression of C1QTNF6 mediated by ncRNAs is related to poor prognosis in patients with ccRCC and might fulfill its partial oncogenic function by increasing tumor immune cells infiltration and immune checkpoints expression.
WITHDRAWN: A comparison of national skin disease burden with standardized exam content for dermatology physician assistants
Authors: Young Peter A. , Prajapati Stuti , Bae Gordon H. , Feldman Steven 
Published: May 2023

Abstract
The dermatology physician assistant (PA) workforce is growing rapidly. The National Commission for Certification of PAs released a standardized written exam in spring of 2023, to formally verify the competence of PA dedicated to dermatology. How well this test prepares PAs to meet the needs of their future patients is not well known. We compared this exam’s content blueprint with existing data on the burden of skin disease in the United States, to characterize whether examinees will be tested on skin disease which significantly affect their presumed patient population. Our findings suggest reasonable concordance between written exam content and the actual burden of skin disease, with some potential areas for refinement based on the high prevalence and mortality of skin cancers, which might presently be under-tested.
Changes in retinal vascular density measured by optical coherence tomography angiography in patients with carotid artery stenosis after carotid artery stenting and angioplasty
Authors: Karapapak Murat , Ermiş Serhat , Bolat Petek Aksöz , Cingöz Mehmet , Erdim Çağrı , Özal Ece , Özal Sadık Altan 
Published: May 2023

Abstract
Purpose
The aim of this study was to compare the effect of carotid artery stenting and angioplasty (CASA) on retinal vascular density (VD) in patients with severe carotid stenosis with a healthy control group and to evaluate using optical coherence tomography angiography (OCTA).
Methods
For this prospective study, eyes on the operated side constituted the ipsilateral eye group, and the other eye constituted the contralateral eye group. 40 eyes of 40 patients with ipsilateral eye of carotid artery stenosis (CAS), 34 eyes on contralateral side, and 30 healthy eyes (control group) were included in this study. We performed quantitative OCTA analyses of retinal VD changes, before and after CASA. The main outcome measures were the quantitative changes of VD of superficial capillary plexus (SCP) and deep capillary plexus (DCP).
Results
We evaluated the VD of ipsilateral eyes and contralateral eyes separately before and after the procedure. All patients did not have visual symptoms. There was no significant difference in the VD of SCP in all groups before the procedure. No significant change was observed in all groups when the VD of the SCP was compared before and after the procedure. The VD of the DCP in the ipsilateral and contralateral group improved significantly after CASA.
Conclusion
OCTA could noninvasively detect retinal VD improvements after CASA in CAS patients. Quantitative changes in VD evaluated using OCTA are thought to be early indicators in the diagnosis of CAS and in the follow-up of treatment success.
A Socio-metabolic Perspective on the Urbanization Process: Material Flows and the Ecological Rucksack
Authors: Contreras-Escandón Christian , Flores Lisseth , Albarracin Giovanni 
Published: May 2023

AbstractUrbanization is experiencing an accelerated development due to population growth and social metabolism. Since society needs nature to produce and consume, transforming matter and energy entropically has generated socio-environmental inequalities and pollution. For this research, we propose two biophysical indicators as methods to measure socio-environmental problems: 1) Analysis of Material Flows (AMF) and 2) Ecological Rucksack (ER) to quantify the flows of the leading materials involved in the construction process of urbanization. As a result, we found poor materials management, waste generation, and consequent environmental pollution. Finally, we recommended that managing these indicators (AFM and ER) at design levels of urbanization could anticipate the difficulties that these generate in the environment. In addition, modelling future scenarios can be helpful to improve relations with nature and reduce socio-environmental impacts.
Developing a TaqMan real-time RT-PCR protocol for detecting rice stripe necrosis virus in plant material and soil
Authors: Moz Brenda , Almeida Roberson Diego Souza , Favero Débora , de Moraes Marcelo Gravina , Bertolini Edson 
Published: May 2023

Abstract
Rice is one of the main staple foodstuffs for the world's population; however, several diseases caused by phytopathogens cause yield losses in rice crop. Rice crinkle is an emerging disease in rice cultivation, caused by rice stripe necrosis virus (RSNV). RSNV is transmitted by the soil plasmodiophoromycete, Polymyxa graminis, which can remain in the soil through resistance structures for decades. The symptoms caused by the virus are often confused with other causes, making a correct diagnosis and the adoption of effective management and control measures difficult. RSNV is detected through tests such as plate trapped antigen – enzyme-linked immunosorbent assay (PTA-ELISA) and conventional reverse transcription polymerase chain reaction RT-PCR. The objective of this study was to develop a protocol for RSNV detection via real-time RT-PCR using a TaqMan probe associated with direct sample preparation methods, which has not been published to date. The RT-PCR detection protocol developed was 100–1,000 times more sensitive than conventional RT-PCR, allowing for the detection of RSNV in contaminated plant material and soil. The direct sample preparation method associated with real-time RT-PCR exhibited similar sensitivity to the nucleic acid purification methods.