bluuebunny/crossref_metadata_2025_split huggingface dataset:
The potential roles of cigarette smoke-induced extracellular vesicles in oral leukoplakia
Authors: Peng Qiao , Duan Ning , Wang Xiang , Wang Wenmei 
Published: July 2023

Abstract
Background
The onset of oral leukoplakia (OLK), the most common oral lesion with a high risk of malignant transformation, is closely associated with the exposure of cigarette smoke. Cigarette smoke is a complicated mixture of more than 4500 different chemicals including various oxidants and free radical, which contributes to the onset of immune and inflammatory response or even carcinogenesis. Recent studies have proved that the exposure of cigarette smoke leads to the onset and aggravation of many diseases via significantly changed the production and components of extracellular vesicles. The extracellular vesicles are membrane-enclosed nanosized particles secreted by diverse cells and involved in cell–cell communication because of their ability to deliver a number of bioactive molecules including proteins, lipids, DNAs and RNAs. Getting insight into the mechanisms of extracellular vesicles in regulating OLK upon cigarette smoke stimulation contributes to unravel the pathophysiology of OLK in-depth. However, evidence done on the role of extracellular vesicles in cigarette smoke-induced OLK is still in its infancy.

Materials and methods
Relevant literatures on cigarette smoke, oral leukoplakia and extracellular vesicles were searched in PubMed database.

Conclusions
In this review, we summarize the recent findings about the function of extracellular vesicles in the pathogenesis of cigarette smoke-induced diseases, and to infer their potential utilizations as diagnostic biomarkers, prognostic evaluation, and therapeutic targets of OLK in the future.
Multi-omics analysis uncovers clinical, immunological, and pharmacogenomic implications of cuproptosis in clear cell renal cell carcinoma
Authors: Zhu Maoshu , Li Yongsheng , Wang Yun , Lin Pingli , Mi Jun , Zhong Weimin 
Published: July 2023

Abstract
Objective
The latest research proposed a novel copper-dependent programmed cell death named cuproptosis. We aimed to elucidate the influence of cuproptosis in clear cell renal cell carcinoma (ccRCC) from a multi-omic perspective.

Methods
This study systematically assessed mRNA expression, methylation, and genetic alterations of cuproptosis genes in TCGA ccRCC samples. Through unsupervised clustering analysis, the samples were classified as different cuproptosis subtypes, which were verified through NTP method in the E-MTAB-1980 dataset. Next, the cuproptosis score (Cuscore) was computed based on cuproptosis-related genes via PCA. We also evaluated clinical and immunogenomic features, drug sensitivity, immunotherapeutic response, and post-transcriptional regulation.

Results
Cuproptosis genes presented multi-layer alterations in ccRCC, and were linked with patients’ survival and immune microenvironment. We defined three cuproptosis subtypes [C1 (moderate cuproptosis), C2 (low cuproptosis), and C3 (high cuproptosis)], and the robustness and reproducibility of this classification was further proven. Overall survival was best in C3, moderate in C1, and worst in C2. C1 had the highest sensitivity to pazopanib, and sorafenib, while C2 was most sensitive to sunitinib. Furthermore, C1 patients benefited more from anti-PD-1 immunotherapy. Patients with high Cuscore presented the notable survival advantage. Cuscore was highly linked with immunogenomic features, and post-transcriptional events that contributed to ccRCC development. Finally, several potential compounds and druggable targets (NMU, RARRES1) were selected for low Cuscore group.

Conclusion
Overall, our study revealed the non-negligible role of cuproptosis in ccRCC development. Evaluation of the cuproptosis subtypes improves our cognition of immunogenomic features and better guides personalized prognostication and precision therapy.
Diabetes distress and disordered eating behaviors in youth with type 1 diabetes: the mediating role of self-regulatory fatigue and the moderating role of resilience
Authors: Zhou Meijing , Wang Hong , Yu Jian , Luo Dan , Zhu Min , Zhang Mei , Xu Jingjing , Yang Tao 
Published: July 2023

Abstract
Background
Despite previous research on the association between diabetes distress and disordered eating behaviors (DEBs) among youth with type 1 diabetes (T1D), there is a lack of understanding regarding the underlying mechanisms. This study aimed to investigate the relationships between diabetes distress and DEBs, specifically examining whether self-regulatory fatigue mediated the relationship and whether resilience moderated this mediation.

Methods
A cross-sectional study was performed among youth with T1D recruited from two diabetes centers in Nanjing, China. Measurement instruments included the problem areas in the diabetes-5 scale, the diabetes strengths and resilience measure for adolescents, the self-regulatory fatigue scale, and the Chinese version of diabetes eating problem survey-revised. Mediation and moderated mediation analyses were conducted.

Results
A total of 185 youths with T1D were involved in the current study. The results indicated that diabetes distress positively predicted DEBs. Self-regulatory fatigue partially mediated the association between diabetes distress and DEBs, accounting for 50.88% of the overall effect. Additionally, the pathway from self-regulatory fatigue to DEBs was moderated by resilience.

Conclusion
The current study examined whether self-regulatory fatigue mediated the relationship between diabetes distress and DEBs and whether resilience moderated the connection between self-regulatory fatigue and DEBs. These findings add to the theoretical basis of how diabetes distress influences DEBs and help guide the incorporation of diabetes distress, self-regulatory fatigue, and resilience into DEBs reduction programs for youth with T1D.
The IBER study: a feasibility randomised controlled trial of imagery based emotion regulation for the treatment of anxiety in bipolar disorder
Authors: Steel Craig , Wright Kim , Goodwin Guy M. , Simon Judit , Morant Nicola , Taylor Rod S. , Brown Michael , Jennings Susie , Hales Susie A. , Regan Jemma , Sibsey Michaela , Thomas Zoe , Meredith Lynette , Holmes Emily A. 
Published: July 2023

Abstract
Background
Intrusive mental imagery is associated with anxiety and mood instability within bipolar disorder and therefore represents a novel treatment target. Imagery Based Emotion Regulation (IBER) is a brief structured psychological intervention developed to enable people to use the skills required to regulate the emotional impact of these images.

Methods
Participants aged 18 and over with a diagnosis of bipolar disorder and at least a mild level of anxiety were randomly assigned (1:1) to receive IBER plus treatment as usual (IBER + TAU) or treatment as usual alone (TAU). IBER was delivered in up to 12 sessions overs 16 weeks. Clinical and health economic data were collected at baseline, end of treatment and 16-weeks follow-up. Objectives were to inform the recruitment process, timeline and sample size estimate for a definitive trial and to refine trial procedures. We also explored the impact on participant outcomes of anxiety, depression, mania, and mood stability at 16-weeks and 32-weeks follow-up.

Results
Fifty-seven (28: IBER + TAU, 27: TAU) participants from two sites were randomised, with 50 being recruited within the first 12 months. Forty-seven (82%) participants provided outcome data at 16 and 32-weeks follow-up. Thirty-five participants engaged in daily mood monitoring at the 32-week follow-up stage. Retention in IBER treatment was high with 27 (96%) attending ≥ 7 sessions. No study participants experienced a serious adverse event.

Discussion
The feasibility criteria of recruitment, outcome completion, and intervention retention were broadly achieved, indicating that imagery-focused interventions for bipolar disorder are worthy of further investigation.
Influence of extreme fracture flow channels on the thermal performance of open-loop geothermal systems at commercial scale
Authors: Rangel-Jurado Nicolás , Hawkins Adam J. , Fulton Patrick M. 
Published: July 2023

AbstractAdequate stewardship of geothermal resources requires accurate forecasting of long-term thermal performance. In enhanced geothermal systems and other fracture-dominated reservoirs, predictive models commonly assume constant-aperture fractures, although spatial variations in aperture can greatly affect reservoir permeability, fluid flow distribution, and heat transport. Whereas previous authors have investigated the effects of theoretical random aperture distributions on thermal performance, here we further explore the influence of permeability heterogeneity considering field-constrained aperture distributions from a meso-scale field site in northern New York, USA. Using numerical models of coupled fluid flow and heat transport, we conduct thermal–hydraulic simulations for a hypothetical reservoir consisting of a relatively impervious porous matrix and a single, horizontal fracture. Our results indicate that in highly channelized fields, most well design configurations and operating conditions result in extreme rates of thermal drawdown (e.g., 50% drop in production well temperatures in under 2 years). However, some other scenarios that account for the risks of short-circuiting can potentially enhance heat extraction when mass flow rate is not excessively high, and the direction of geothermal extraction is not aligned with the most permeable features in the reservoir. Through a parametric approach, we illustrate that well separation distance and relative positioning play a major role in the long-term performance of highly channelized fields, and both can be used to help mitigate premature thermal breakthrough.
Municipal green waste as substrate for the microbial production of platform chemicals
Authors: Volkmar Marianne , Maus Anna-Lena , Weisbrodt Martin , Bohlender Jonathan , Langsdorf Alexander , Holtmann Dirk , Ulber Roland 
Published: July 2023

AbstractIn Germany alone, more than 5·106 tons of municipal green waste is produced each year. So far, this material is not used in an economically worthwhile way. In this work, grass clippings and tree pruning as examples of municipal green waste were utilized as feedstock for the microbial production of platform chemicals. A pretreatment procedure depending on the moisture and lignin content of the biomass was developed. The suitability of grass press juice and enzymatic hydrolysate of lignocellulosic biomass pretreated with an organosolv process as fermentation medium or medium supplement for the cultivation of Saccharomyces cerevisiae, Lactobacillus delbrueckii subsp. lactis, Ustilago maydis, and Clostridium acetobutylicum was demonstrated. Product concentrations of 9.4 gethanol L−1, 16.9 glactic acid L−1, 20.0 gitaconic acid L−1, and 15.5 gsolvents L−1 were achieved in the different processes. Yields were in the same range as or higher than those of reference processes grown in established standard media. By reducing the waste arising in cities and using municipal green waste as feedstock to produce platform chemicals, this work contributes to the UN sustainability goals and supports the transition toward a circular bioeconomy.
Graphical Abstract
Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique
Authors: Izanlou Zahra , Akhavan Mahdavi Mahmood , Gheshlaghi Reza , Karimian Arash 
Published: July 2023

AbstractIn this study, the sequential extraction of the three types of biochemicals from microalgae is employed, which is a more realistic and practical solution for large-scale extraction of bioproducts. The drying, grinding, organic solvent treatment, and ultra-sonication were combined to disrupt cells and sequentially extract bioproducts from three microalgae strains, Chlorella sorokiniana IG-W-96, Chlorella sp. PG-96, and Chlorella vulgaris IG-R-96. As the drying is the most energy-intensive step in cell disruption and sequential extraction, the effect of this step on sequential extraction deeply explored. The results show that total ash-plus contents of biochemicals in freeze-dried samples (95.4 ± 2.8%, 89.3 ± 3.9%, and 77.5 ± 4.2 respectively) are higher than those in oven-dried samples (91.0 ± 2.8%, 89.5 ± 3.0%, 71.4 ± 4.8%, respectively) showing the superiority of freeze drying over oven drying merely for Chlorella vulgaris IG-R-96 (p-value = 0.003) and non-significant variation for Chlorella sorokiniana IG-W-96 (p-value = 0.085) and Chlorella sp. PG-96 (p-value = 0.466). Variation among biochemical contents of strains is due to the difference in cell wall strength confirmed by TEM imaging. The freeze-dried samples achieved higher lipid yields than oven-dried samples. The total carbohydrate yields followed the same pattern. The extraction yields of total protein were higher in freeze-dried samples than in oven-dried. Total mass balance revealed that drying-based sequential extraction of value-added bioproducts could better demonstrate the economic potential of sustainable and renewable algal feedstock than independent assays for each biochemical.
Graphical Abstract
Microbiome specificity and fluxes between two distant plant taxa in Iberian forests
Authors: Saati-Santamaría Zaki , Vicentefranqueira Rocío , Kolařik Miroslav , Rivas Raúl , García-Fraile Paula 
Published: July 2023

Abstract
Background
Plant-associated microbial communities play important roles in host nutrition, development and defence. In particular, the microbes living within internal plant tissues can affect plant metabolism in a more intimate way. Understanding the factors that shape plant microbial composition and discovering enriched microbes within endophytic compartments would thus be valuable to gain knowledge on potential plant–microbial coevolutions. However, these interactions are usually studied through reductionist approaches (in vitro models or crop controlled systems). Here, we investigate these ecological factors in wild forest niches using proximally located plants from two distant taxa (blueberry and blackberry) as a model.

Results
Although the microbial communities were quite similar in both plants, we found that sampling site had a high influence on them; specifically, its impact on the rhizosphere communities was higher than that on the roots. Plant species and sample type (root vs. rhizosphere) affected the bacterial communities more than the fungal communities. For instance, Xanthobacteraceae and Helotiales taxa were more enriched in roots, while the abundance of Gemmatimonadetes was higher in rhizospheres. Acidobacteria abundance within the endosphere of blueberry was similar to that in soil. Several taxa were significantly associated with either blackberry or blueberry samples regardless of the sampling site. For instance, we found a significant endospheric enrichment of Nevskia in blueberry and of Sphingobium, Novosphingobium and Steroidobacter in blackberry.

Conclusions
There are selective enrichment and exclusion processes in the roots of plants that shapes a differential composition between plant species and sample types (root endosphere—rhizosphere). The special enrichment of some microbial taxa in each plant species might suggest the presence of ancient selection and/or speciation processes and might imply specific symbiosis. The selection of fungi by the host is more pronounced when considering the fungal trait rather than the taxonomy. This work helps to understand plant–microbial interactions in natural ecosystems and the microbiome features of plants.
Storytelling Podcast as an Alternative Learning Tool for Early Childhood Education: A TPACK-Based Reflection
Authors: Formen Ali , Waluyo Edi 
Published: July 2023

Purpose – This article reports on a project to facilitate teachers’ digital skill improvement. The context of the project itself was the past COVID-19 pandemic, which paralized the education sector, including the closure of early childhood education (ECE) centers. While remote, online learning was highly recommended to face disastrous situation such as pandemic, in practice, especially at the ECE level, online learning faces complex challenges. On the other hand, teachers were not adequately prepared for online and digital learning.
Design/methods/approach – This study combined developmental and conventional descriptive methods. The participants (15 people) were teachers, with various backgrounds of work experience (2 to over 7 years), with good internet access skills. Data and information were collected through observation, interviews, and documentation; followed by descriptive analysis.
Findings – These teachers found that Podcasts could be an alternative and a variation of digital learning media. Making and using podcasts can also be an entry point in strengthening the integration of Technological and Pedagogical Content Knowledge (TPACK) in the ECE context.
Research implications/limitations – While storytelling Podcast as ECE learning tool was used limitedly during the COVID-19 period, its uses may last beyond the pandemic time provided systematic integration of digital technology and skills associated with it into ECE sector is met.
Practical implications – This study implies that storytelling podcasts can be an alternative learning tool in the situation where teacher-student face-to-face interaction is less possible.
Originality/value – The emergency period prevented teacher-student direct contacts. This study contributes to providing an alternative to learning in an emergency situation.
Paper type Research paper
Incomplete filling of spray-dried K2EDTA evacuated blood tubes: impact on measuring routine hematological parameters on Sysmex XN-10
Authors: Lippi Giuseppe , Dima Francesco , Tosi Marco , Carpenè Giovanni , Celegon Giovanni , Favaloro Emmanuel J. , Salvagno Gian Luca 
Published: July 2023

Abstract

Objectives
Because there is little published evidence on the effects of incomplete filling of K2EDTA evacuated blood tubes on routine hematological testing, this original study aimed to provide updated information on this preanalytical aspect.

Methods
The study population consisted of 17 ostensibly healthy volunteers. Blood was drawn by venipuncture with a 10 mL syringe and dispensed in varying amounts (0.2, 0.5, 1.0, 2.0, and 3.0 mL) into 3.0 mL blood tubes containing spray-dried 5.4 mg K2EDTA. All tubes were gently mixed and used to perform routine hematology tests on the Sysmex XN-10. Clinically significant variations were defined when the limits of desirable specifications of bias derived from biologic variation were exceeded.

Results
The desirable bias was exceeded in 33 % filled tubes (1.0 mL) for hematocrit and MCV (increased values) and for MCHC (decreased values), while it was exceeded in 17 % filled tubes (0.5 mL) for hemoglobin, hematocrit and MCV (increased values), and for MCHC (decreased values). Finally, the variation of values was higher than the desirable bias for RBC, hemoglobin, hematocrit and MCV (increase), and for MCHC and MPV (decrease) in 7 % filled tubes (0.2 mL). No clinically significant variations were observed in tubes filled up to 67 % of their nominal volume (i.e., 2.0 mL).

Conclusions
Consideration should be given to reject spray-dried K2EDTA blood tubes that contain a blood volume <67 % of the nominal fill volume, as biased laboratory data in these samples may interfere with clinical decision making and care management.
Inside the commentator’s booth: a Multimodal (Inter)action and Conversation Analysis on the production of first division football commentary on TV
Authors: Du Bois Inke 
Published: July 2023

Abstract
This paper discusses the linguistic features of the production of football commentary within its macrosocial contexts. It contrasts the communication of the TV production team during a football game with the live football commentary aired on TV. Applying a mixed methods analysis, it reveals how football commentary is prepared and produced in the technical setting of the commentator’s booth in a stadium. This study reveals how a medial reality of the same events concerning a goal are broadcasted in the 11 min highlight video football commentary. The author and her co-principal investigator video recorded a total of 36 h in four work settings. Finally, retrospective interviews with the commentator and head of sports of the TV station place the study in the broader social context of football as a media production. In this paper, I show how live football reporting is prepared and conducted using two different theoretical and methodological frameworks: Conversation Analysis (CA) and Multimodal (Inter)action Analysis (MMIA). Both provide insight into the synthetic verbal, non-verbal and parallel actions for the successful production of the football commentary and they also reveal the sequential structure of naturally occurring talk (CA).
The Use of Steel Slags in Transport Infrastructures: A Critical Review
Authors: Mica Nelson Gomes , Rios Sara , Viana da Fonseca António , Fortunato Eduardo 
Published: July 2023

Abstract
The increasing demand for the development and expansion of transport infrastructures requires alternative solutions that are economically and environmentally viable to provide the necessary raw materials. Transport infrastructures consume a large amount of natural aggregates; however, from a circular economy perspective, industrial by-products can effectively replace these materials. This avoids the environmental impacts associated with the extraction of natural raw materials and simultaneously provides a destination for the industrial by-products. For this purpose, it is important to evaluate if existing standards and technical requirements that were developed for natural aggregates are suitable for industrial by-products. This review study is focused on slags from steelmaking process in electric arc furnaces, which are generally of two types: oxidizing (black colored) and reducing (lighter colored), specifically addressing the requirements of these materials for the granular layers of transport infrastructures. The chemical composition of the slags is analyzed as well as its physical and mechanical characteristics, swelling behavior, and leachate content, discussing testing procedures and technical specifications.
Research on Engineering Performance of Paving Mixes with Multi-component SBS Modifier under Dry Modification
Authors: Xu Ning , Wang Hainian , Zhang Peng , Li Yuanle , Wang Huimin , Jiao Hu , Liu Shenglan 
Published: July 2023

Abstract
Dry modification of modified asphalt mixtures prepared by mixing the modifier with the aggregate and asphalt binder is a simple and environmentally friendly process. It can avoid the segregation of modified asphalt used for paving mixes under wet modification mode. The difficulty of rapid swelling makes it difficult for the styrene-butadiene-styrene block copolymer (SBS) modifier to achieve dry modification. This research attempted to prepare a multi-component SBS-based (SBSM) modifier for dry modification by blending SBS with assisting ingredients through a twin-screw extruder. The conventional properties, rheological properties, and thermal storage stability of SBSM-modified asphalt were evaluated. Additionally, the road performance of SBSM-modified asphalt mixtures prepared by both wet modification and dry modification was characterized. The results show that SBSM-modified asphalt obtains excellent high-temperature and low-temperature properties as well as thermal storage stability. Satisfactory performance in terms of resistance to high-temperature rutting, low-temperature cracking, and water damage is obtained when the SBSM modifier was applied in modified asphalt mixtures under wet modification. Compared with the SBSM-modified asphalt mixture under wet modification, the road performance of the SBSM-modified asphalt mixture under dry modification is slightly inferior, especially the water stability, but satisfies the engineering requirements. The findings demonstrate the feasibility of the dry modification of the SBSM modifier for paving mixtures and promote the development of SBS-based modifiers for dry modification.