bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATRA sensitized the response of hepatocellular carcinoma to Sorafenib by downregulation of p21-activated kinase 1
Authors: Wang Kai , Qiu Xun , Zhang Zhensheng , Xu Hanzhi , Tan Yawen , Su Renyi , Gao Fengqiang , Zhuo Jianyong , Li Wangyao , Lian Zhengxing , He Hong , Xu Xiao 
Published: August 2023

Abstract
Background
Sorafenib resistance greatly reduces the efficacy of treatments in advanced hepatocellular carcinoma (HCC) patients, but the underlying mechanisms are not thoroughly understood. All-trans retinoic acid (ATRA), an anti-leukaemia agent, has attracted considerable attention due to its role in sensitizing cells to other anticancer treatments. We aimed to investigate the combined effect of ATRA and Sorafenib on HCC and the underlying mechanisms.

Methods
CCK-8, cell sphere formation, trans-well migration, and wound-healing assays were used to analyse the biological behaviours of HCC cells in vitro. Western blotting and qRT-PCR analysis were conducted to measure the expression of p21 activated kinase 1 (PAK1) and phospho-p21 activated kinase 1 (pPAK1). Xenograft models were established to confirm the synergistic effects of ATRA and Sorafenib in vivo. TUNEL assays and immunohistochemistry were utilized to determine apoptosis, proliferation, PAK1 and pPAK1 levels in tumour tissues.

Results
We observed that PAK1 was overexpressed in HCC, and its expression was negatively correlated with the survival of patients. PAK1 promoted the proliferation, self-renewal and epithelial-mesenchymal transition of HCC cells. Correlation analysis indicated that the IC50 of Sorafenib was positively correlated with the level of pPAK1 in HCC cell lines. ATRA inhibited the progression of HCC and sensitized HCC response to Sorafenib by downregulation of PAK1, as shown by the calculated coefficient of drug interaction and the data obtained from xenograft models.

Conclusions
Our findings indicated that instead of treatment with Sorafenib alone, the combination of ATRA and Sorafenib provides a more effective treatment for HCC patients.
Systems analysis and improvement approach to improve naloxone distribution within syringe service programs: study protocol of a randomized controlled trial
Authors: Akiba Christopher F. , Patel Sheila V. , Wenger Lynn D. , Morgan-Lopez Antonio , Zarkin Gary A. , Orme Stephen , Davidson Peter J. , Kral Alex H. , Lambdin Barrot H. 
Published: August 2023

Abstract
Background
More than half a million Americans died of an opioid-related overdose between 1999 and 2020, the majority occurring between 2015 and 2020. The opioid overdose mortality epidemic disproportionately impacts Black, Indigenous, and people of color (BIPOC): since 2015, overdose mortality rates have increased substantially more among Black (114%) and Latinx (97%) populations compared with White populations (32%). This is in part due to disparities in access to naloxone, an opioid antagonist that can effectively reverse opioid overdose to prevent death. Our recent pilot work determined that many barriers to naloxone access can be identified and addressed by syringe service programs (SSPs) using the Systems Analysis and Improvement Approach to Naloxone distribution (SAIA-Naloxone). This randomized controlled trial will test SAIA-Naloxone’s ability to improve naloxone distribution in general and among BIPOC specifically.

Methods
We will conduct a trial with 32 SSPs across California, randomly assigning 16 to the SAIA-Naloxone arm and 16 to receive implementation as usual. SAIA-Naloxone is a multifaceted, multilevel implementation strategy through which trained facilitators work closely with SSPs to (1) assess organization-level barriers, (2) prioritize barriers for improvement, and (3) test solutions through iterative change cycles until achieving and sustaining improvements. SSPs receiving SAIA-Naloxone will work with a trained facilitator for a period of 12 months. We will test SAIA-Naloxone’s ability to improve SSPs’ naloxone distribution using an interrupted time series approach. Data collection will take place during a 3-month lead-in period, the 12-month active period, and for an additional 6 months afterward to determine whether impacts are sustained. We will use a structured approach to specify SAIA-Naloxone to ensure strategy activities are clearly defined and to assess SAIA-Naloxone fidelity to aid in interpreting study results. We will also assess the costs associated with SAIA-Naloxone and its cost-effectiveness.

Discussion
This trial takes a novel approach to improving equitable distribution of naloxone amid the ongoing epidemic and associated racial disparities. If successful, SAIA-Naloxone represents an important organizational-level solution to the multifaceted and multilevel barriers to equitable naloxone distribution.
Treatment progress of spinal metastatic cancer: a powerful tool for improving the quality of life of the patients
Authors: Zhao Yuliang , Liu Fei , Wang Wei 
Published: August 2023

AbstractSpinal metastasis is a common secondary malignant tumor of the bone, often resulting in spinal cord and nerve root compression, leading to obvious pain and related compression symptoms. This condition has a high incidence and mortality rate. The treatment approach for most patients with spinal metastasis is primarily palliative. Consultation with a multidisciplinary team is widely accepted as a comprehensive treatment approach for patients with spinal metastases. With advancements in research and technology, the evaluation and treatment of spinal metastatic cancer are continuously evolving. This study provides an overview of surgical treatment, minimally invasive treatment, and radiotherapy for spinal metastatic cancer and also analyzes the clinical effects, advantages, and current limitations associated with various treatment approaches.
The performance of duplex ultrasonography for the assessment of renal artery stenosis in Takayasu’s arteritis patients
Authors: Wang Yahong , Wang Ying , Zhang Li , Ge Zhitong , Li Jing , Yang Yunjiao , Chen Yu , Yang Xiao , Li Jianchu , Tian Xinping 
Published: August 2023

Abstract
Background
This study intends to analyze the hemodynamic parameters of the renal artery in patients with Takayasu’s arteritis (TAK) to explore the diagnostic efficacy of duplex ultrasonography in assessing the involved renal artery in TAK patients.

Methods
One hundred fifteen TAK patients with 314 renal arteries were retrospectively analyzed, who were admitted to Peking Union Medical College Hospital between 2017 and 2022. These patients underwent both renal artery ultrasonography and angiography within a 4-week period. Specifically, the study compared seven ultrasonic parameters across groups categorized by the severity of renal artery stenosis (RAS), including noninvolvement, < 50% stenosis, 50–69% stenosis, and 70–99% stenosis. Receiver operating characteristic (ROC) curves were employed to determine the optimal threshold values for renal artery peak systolic velocity (RPSV), renal-aortic PSV ratio (RAR), and renal-interlobar PSV ratio (RIR) in order to diagnose various degrees of RAS in TAK patients.

Results
Statistically significant differences were observed in RAR and RIR among the four groups (all P < 0.05). However, no statistically significant differences were found in RPSV and AT between the moderate stenosis group (50–69% stenosis) and the severe stenosis group (70–99% stenosis). The discrimination of interlobar PSV (IPSV) and interlobar RI (IRI) was not significant, and IEDV did not show statistical significance among the four groups.
For TAK patients, the recommended thresholds of RPSV for the diagnosis of renal artery involvement (RAI), ≥ 50% RAS, and ≥ 70% RAS were determined to be 143 cm/s, 152 cm/s, and 183 cm/s, respectively. The sensitivities, specificities, and accuracies of these thresholds were all found to be greater than 80%. Additionally, the optimal thresholds of RIR for detecting RAI, ≥ 50% RAS, and ≥ 70% RAS were determined to be 4.6, 5.6, and 6.4, respectively, with satisfactory diagnostic efficiencies. The areas under the curve (AUCs) for RPSV and RIR were calculated to be 0.908 and 0.910, respectively, for the diagnosis of ≥ 50% RAS, and 0.876 and 0.882 for the diagnosis of ≥ 70% RAS. When the aortic PSV is greater than or equal to 140 cm/s, the RAR exhibits inadequate diagnostic efficacy. Conversely, when the aortic PSV is less than 140 cm/s, a RAR value of 2.2 or higher can be employed as the diagnostic threshold for identifying RAS of 70% or greater, with a sensitivity of 84.00%, specificity of 89.93%, and an overall accuracy of 89.08%.

Conclusion
In the present study, it has been demonstrated that RPSV and RIR possess substantial diagnostic value as ultrasonic parameters for diagnosing RAS in TAK patients. Furthermore, when assessing the diagnostic efficacy of RAR, it is crucial to consider the severity of aortic stenosis as a determining factor.
Mapping the effectiveness of the community tuberculosis care programs: a systematic review
Authors: Sejie Gabalape Arnold , Mahomed Ozayr H. 
Published: August 2023

Abstract
Background
Tuberculosis is a significant global public health threat, especially in countries with limited resources. To improve tuberculosis care, the World Health Organization emphasizes the importance of considering a TB patient’s journey across a variety of connected settings and facilities. A systematic review was conducted to map previously conducted studies to identify existing community TB implementation models, their effectiveness on cost, and treatment outcomes.

Methods
Systematic search through various electronic databases MEDLINE, EBSCO (PsycINFO and CINAHL), Cochrane Library, EMBASE, WHO Regional Databases, gray literature, and hand-searched bibliographies was performed. Articles published in English between the years 2000 and 2022 with a substantial focus on community TB implementation models were considered for inclusion. Studies were excluded if the intervention was purely facility-based and those focusing exclusively on qualitative assessments. Two reviewers used standardized methods to screen titles, abstracts, and data charting. Included studies were assessed for quality using ROBINS-I and ROB 2. Analysis of study results uses a PRISMA flow diagram and quantitative approach.

Results
A total of 6982 articles were identified with 36 meeting the eligibility criteria for analysis. Electronic medication monitors showed an increased probability of treatment success rate (RR 1.0–4.33 and the 95% CI 0.98–95.4) in four cohort studies in low- and middle-income countries with the incremental cost-effectiveness of $434. Four cohort studies evaluating community health worker direct observation therapy in low- and middle-income countries showed a treatment success risk ratio of up to 3.09 with a 95% CI of 0.06–7.88. (32,41,43,48) and incremental cost-effectiveness up to USS$410. Moreover, four comparative studies in low- and middle-income countries showed family directly observed treatment success risk ratio up to 9.07, 95% CI of 0.92–89.9. Furthermore, four short message service trials revealed a treatment success risk ratio ranging from 1.0 to 1.45 (95% CI fell within these values) with a cost-effectiveness of up to 350I$ compared to standard of care.

Conclusions
This review illustrates that community-based TB interventions such as electronic medication monitors, community health worker direct observation therapy, family directly observed treatment, and short message service can substantially bolster efficiency and convenience for patients and providers while reducing health system costs and improving clinical outcomes.
Global regularity of 2D MHD equations with almost Laplacian velocity dissipation
Authors: Li Linrui , Hong Mingli 
Published: August 2023

AbstractWe obtain the global existence and global regularity for the 2D MHD equations with almost Laplacian velocity dissipation, which require the dissipative operators weaker than any power of the fractional Laplacian. The result can be regarded as a further improvement and generalization of previous works.
How effective are perches in promoting bird-mediated seed dispersal for natural forest regeneration? A systematic review protocol
Authors: Gan Jelaine Lim , Grainger Matthew James , Shirley Mark David Foster , Pfeifer Marion 
Published: August 2023

Abstract
Background
Forest landscape restoration (FLR), often through tree planting, is one of the priorities in many global and national initiatives for carbon offsetting as part of climate change mitigation and biodiversity conservation. However, active efforts to meet FLR objectives entail substantial costs for the procurement of planting stocks and require an experienced workforce for planting and nurturing tree seedlings. Alternatively, restoration projects can be more cost-effective and potentially may have greater biodiversity gain through assisting and accelerating natural forest regeneration. The use of perches is one of the strategies under Assisted Natural Regeneration (ANR) and is used to attract avian seed dispersers to degraded habitats for increased tree seed supply and seedling establishment. This systematic review and potential meta-analysis aim to determine the effectiveness of artificial and natural perches in promoting natural forest regeneration. Specifically, we will evaluate their effectiveness in driving seed richness, seed density, seedling richness, and seedling density. The results will synthesize available evidence on the topic, identify knowledge gaps we need filling to upscale the strategy, and inform their use in concert with other ANR strategies.

Methods
The search strategy was informed through a literature scan and discussions with stakeholders and experts. A total of eight databases, which include an organizational library and a web-based search engine, will be searched using the refined search string in English. The search string was formed using keywords corresponding to the PICO structure of the research question, and its comprehensiveness was evaluated using 10 benchmark articles. The search results will be screened by the review team (composed of a primary and at least two secondary reviewers) using the set eligibility criteria at the title and abstract level, followed by the full-text screening. The screened studies will then undergo critical appraisal using the assessment criteria based on risk of bias and methods. Data from the accepted studies will be extracted to the standard data sheet for meta-analysis. Effect size (Hedges’ g) will be computed to determine whether perches are effective in increasing seed dispersal and seedling establishment in degraded sites. The effect of potential modifiers relating to the landscape will be explored via mixed models.
MicroRNA-144-3p protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles by targeting MAP3K9
Authors: Liu Meng , Xiao Bang , Zhu Yiqing , Chen Meiting , Huang Jinfeng , Guo Haiyan , Wang Fang 
Published: August 2023

AbstractPremature ovarian failure (POF) is defined by amenorrhea, ovarian atrophy, hypoestrogenism, elevated gonadotropin level, and infertility under the age of 40. POF is frequently induced by chemotherapeutic agents. However, the underlying mechanisms regarding chemotherapy-mediated damage to ovarian function are unclear. In this study, enhanced apoptosis of granulosa cells (GCs) and aberrant activation of primordial follicles were observed in a POF mouse model induced by cisplatin. We subsequently observed significant downregulation of miR-144-3p and upregulation of mitogen-activated protein kinase kinase kinase 9 (MAP3K9) in primary ovarian GCs from POF mice, as revealed by microarrays. Furthermore, MAP3K9 expression was higher in human ovarian granulosa cells (COV434) treated with cisplatin and was identified as a novel target of miR-144-3p. Functional analysis revealed that miR-144-3p attenuated cisplatin induced apoptosis of GCs via silencing MAP3K9 expression, which suppressed the activity of the downstream p38 mitogen activated protein kinase (MAPK) pathway. Meanwhile, miR-144-3p prevented premature primordial follicle depletion in cisplatin-induced POF mice through targeting Map3k9, which led to a decline in the phosphorylation and activation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase b (AKT) pathway. Taken together, this study revealed the protective effects of miR-144-3p on ovarian function and shed light on the epigenetic regulatory mechanism in the development of POF, which might provide new biomarkers for the ovarian reserve.
The role of organoids in cancer research
Authors: Fang Zhen , Li Peijuan , Du Fengying , Shang Liang , Li Leping 
Published: August 2023

AbstractOrganoids are established through in vitro 3D culture, and they can mimic the structure and physiological functions of organs or tissues in vivo. Organoids have attracted much attention in recent years. They can provide a reliable technology platform for cancer research and treatment and are a valuable preclinical model for academic research and personalized medicine. A number of studies have confirmed that organoids have great application prospects in new drug development, drug screening, tumour mechanism research, and precision medicine. In this review, we mainly focus on recent advances in the application of organoids in cancer research. We also discussed the opportunities and challenges facing organoids, hoping to indicate directions for the development of organoids in the future.
The global prevalence of screen-based disordered eating and associated risk factors among high school students: systematic review, meta-analysis, and meta-regression
Authors: Ghazzawi Hadeel A. , Nimer Lana S. , Sweidan Dima H. , Alhaj Omar A. , Abulawi Duha , Amawi Adam T. , Levine Michael P. , Jahrami Haitham 
Published: August 2023

Abstract
Objective
Estimate the prevalence, and associated risk factors, of high school students who are considered at risk for an eating disorder based on screening measures.

Methods
An electronic search of nine databases was completed from their inception until 1st September 2022. A random-effects meta-analysis was conducted, and confounder (moderator) analyses and meta-regressions examined whether the overall prevalence estimate for of screen-based disordered eating (SBDE) was moderated by student age, BMI, or gender, as well as culture and type of SBDE assessment.

Results
The mean estimate of the prevalence of SBDE among high school students (K = 42 (66 datapoints), N = 56282] in the sample of 25 countries was 13% ([95% CI] = 10.0–16.8%, I2 = 99.0%, Cochran's Q p = 0.001). This effect was not moderated by features of the samples such as gender, BMI, or age. Among cultures, non-Western countries had a higher prevalence of SBDE prevalence than Western countries, but the difference was not significant. There was considerable variability in the prevalence estimates as a function of the assessment measure, but no meaningful pattern emerged.

Conclusion
The estimated figure of 1 in 8 high school students with SBDE—unmoderated by gender and BMI—stands out as a problem in need of attention from public health officials, psychologists, psychiatrists, pediatricians, parents, and educators. There is a great need for innovative, integrated policy and program development all along the spectrum of health promotion and universal, selective, and indicated prevention. Further research is also needed to validate and refine this estimate by (a) conducting basic research on the accuracy of eating disorder screening measurements in samples ages 14 through 17; (b) examining representative samples in more countries in general and Latin American countries in particular; (c) clarifying the relationships between SBDE and age throughout the different phases of late childhood, adolescence, and emerging adulthood; and (d) investigating whether there are meaningful forms of disordered eating and whether these are associated with variables such as gender, ethnicity, and BMI.
An investigation of Bellini’s Transfiguration in the Capodimonte museum by means of XRF and visible reflectance hyperspectral imaging: Bellini’s handling of materials in the head and figure of the transfigured Christ
Authors: de Viguerie Laurence , Glanville Helen , Radepont Marie , Cerasuolo Angela , Rullo Alessandra , Seccaroni Claudio , Walter Philippe 
Published: August 2023

Abstract
The Transfiguration by Giovanni Bellini in the Museo di Capodimonte (Naples) is an iconic image of a transcendental event at the heart of the Christian religion. Using non-destructive chemical imaging, and focusing on the central image of the transfigured Christ we have studied by what means and with what materials the artist represented this divine event. The results showed what is to date the earliest use of the pigment stibnite. The techniques employed demonstrate both continuity with Bellini’s earliest works (such as the combination of the use of shell-gold and organic, tinted layers), but also his knowledge of Netherlandish painting. This research has furthered our understanding of how he achieved the luminosity so characteristic of his translation of the divine into the materiality of paint.
How to insert visual information into a whiteboard animation with a human hand? Effects of different insertion styles on learning
Authors: Krieglstein Felix , Meusel Felicia , Rothenstein Eva , Scheller Nadine , Wesenberg Lukas , Rey Günter Daniel 
Published: August 2023

AbstractWhiteboard animations have become very popular in recent years. They are mainly used in distance education, where learners can acquire knowledge individually and without the help of a teacher. However, there is little empirical evidence on how whiteboard animations should be designed to achieve learning-enhancing effects. Since the presentation of whiteboard animations is reminiscent of a teacher drawing or showing content on a whiteboard, the hand has been identified as an essential feature of this learning medium. Therefore, the aim of this experimental study was to investigate whether and how the human hand should be implemented in whiteboard animations for the presentation of visual content. University students (N = 84) watched a whiteboard animation in which the type of information insertion was manipulated (hand drawing content vs. hand pushing content in vs. no hand visible). Results revealed that the drawing hand on a whiteboard led to significantly higher intrinsic motivation than the hand pushing visual content onto the whiteboard. Contrary to assumptions derived from cognitive load theory, the implementation of a human hand did not cause extraneous cognitive load. However, no other effects on the perception of the instructor, cognitive load, and learning performance were found. The results are discussed in terms of both cognitive and social processes in multimedia learning.