bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐acetyltransferase 10 promotes the progression of oral squamous cell carcinoma through N4‐acetylcytidine RNA acetylation of MMP1 mRNA
Authors: Liu Yi , Huang Hui , Zhang Cun‐bao , Fan Hua‐nan 
Published: September 2023

AbstractThe pathogenesis of oral squamous cell carcinoma (OSCC) remains unclear. Therefore, clarifying its pathogenesis and molecular‐level development mechanism has become the focus of OSCC research. N‐acetyltransferase 10 (NAT10) is a crucial enzyme involved in mRNA acetylation, regulating target gene expression and biological functions of various diseases through mediating N4‐acetylcytidine (ac4C) acetylation. However, its role in OSCC progression is not well understood. In this study, we showed that NAT10 was significantly upregulated in OSCC tissues compared to normal oral tissues. Moreover, lentivirus‐mediated NAT10 knockdown markedly suppressed cell proliferation, migration, and invasion in two OSCC cell lines (SCC‐9 and SCC‐15). Interestingly, MMP1 was found to be significantly upregulated in OSCC tissues and was a potential target of NAT10. N‐acetyltransferase 10 knockdown significantly reduced both the total and ac4C acetylated levels of MMP1 mRNA and decreased its mRNA stability. Xenograft experiments further confirmed the inhibitory effect of NAT10 knockdown on the tumorigenesis and metastasis ability of OSCC cells and decreased MMP1 expression in vivo. Additionally, NAT10 knockdown impaired the proliferation, migration, and invasion abilities in OSCC cell lines in an MMP1‐dependent manner. Our results suggest that NAT10 acts as an oncogene in OSCC, and targeting ac4C acetylation could be a promising therapeutic strategy for OSCC treatment.
Cholesterol biogenesis is a PTEN‐dependent actionable node for the treatment of endocrine therapy‐refractory cancers
Authors: Kaysudu Irmak , Gungul Taha Bugra , Atici Sena , Yilmaz Sevval , Bayram Engin , Guven Gozde , Cizmecioglu Nihal Terzi , Sahin Ozgur , Yesiloz Gurkan , Haznedaroglu Berat Zeki , Cizmecioglu Onur 
Published: September 2023

AbstractPTEN and PIK3CA mutations are the most prevalent PI3K pathway alterations in prostate, breast, colorectal, and endometrial cancers. p110β becomes the prominent PI3K isoform upon PTEN loss. In this study, we aimed to understand the molecular mechanisms of PI3K dependence in the absence of PTEN. Using online bioinformatical tools, we examined two publicly available microarray datasets with aberrant PI3K activation. We found that the rate‐limiting enzyme of cholesterol biogenesis, SQLE, was significantly upregulated in p110β‐hyperactivated or PTEN‐deficient mouse prostate tumors. Concomitantly, the expression of cholesterol biosynthesis pathway enzymes was directly correlated with PI3K activation status in microarray datasets and diminished upon PTEN re‐expression in PTEN‐null prostate cancer cells. Particularly, PTEN re‐expression decreased SQLE protein levels in PTEN‐deficient prostate cancer cells. We performed targeted metabolomics and detected reduced levels of cholesteryl esters as well as free cholesterol upon PTEN re‐expression. Notably, PTEN‐null prostate and breast cancer cell lines were more sensitive to pharmacological intervention with the cholesterol pathway than PTEN‐replete cancer cells. Since steroid hormones use sterols as structural precursors, we studied whether cholesterol biosynthesis may be a metabolic vulnerability that enhances antihormone therapy in PTEN‐null castration‐resistant prostate cancer cells. Coinhibition of cholesterol biosynthesis and the androgen receptor enhanced their sensitivity. Moreover, PTEN suppression in endocrine therapy‐resistant luminal‐A breast cancer cells leads to an increase in SQLE expression and a corresponding sensitization to the inhibition of cholesterol synthesis. According to our data, targeting cholesterol biosynthesis in combination with the hormone receptor signaling axis can potentially treat hormone‐resistant prostate and breast cancers.
mTOR signaling and Alzheimer's disease: What we know and where we are?
Authors: Davoody Samin , Asgari Taei Afsaneh , Khodabakhsh Pariya , Dargahi Leila 
Published: September 2023

AbstractDespite the great body of research done on Alzheimer's disease, the underlying mechanisms have not been vividly investigated. To date, the accumulation of amyloid‐beta plaques and tau tangles constitutes the hallmark of the disease; however, dysregulation of the mammalian target of rapamycin (mTOR) seems to be significantly involved in the pathogenesis of the disease as well. mTOR, as a serine–threonine protein kinase, was previously known for controlling many cellular functions such as cell size, autophagy, and metabolism. In this regard, mammalian target of rapamycin complex 1 (mTORC1) may leave anti‐aging impacts by robustly inhibiting autophagy, a mechanism that inhibits the accumulation of damaged protein aggregate and dysfunctional organelles. Formation and aggregation of neurofibrillary tangles and amyloid‐beta plaques seem to be significantly regulated by mTOR signaling. Understanding the underlying mechanisms and connection between mTOR signaling and AD may suggest conducting clinical trials assessing the efficacy of rapamycin, as an mTOR inhibitor drug, in managing AD or may help develop other medications. In this literature review, we aim to elaborate mTOR signaling network mainly in the brain, point to gaps of knowledge, and define how and in which ways mTOR signaling can be connected with AD pathogenesis and symptoms.
Breakdown of oscillatory effective networks in disorders of consciousness
Authors: Bai Yang , Gong Anjuan , Wang Qijun , Guo Yongkun , Zhang Yin , Feng Zhen 
Published: September 2023

AbstractIntroductionCombining transcranial magnetic stimulation with electroencephalography (TMS‐EEG), oscillatory reactivity can be measured, allowing us to investigate the interaction between local and distant cortical oscillations. However, the extent to which human consciousness is related to these oscillatory effective networks has yet to be explored.AimsWe tend to investigate the link between oscillatory effective networks and brain consciousness, by monitoring the global transmission of TMS‐induced oscillations in disorders of consciousness (DOC).ResultsA cohort of DOC patients was included in this study, which included 28 patients with a minimally conscious state (MCS) and 20 patients with vegetative state/unresponsive wakefulness syndrome (VS/UWS). Additionally, 25 healthy controls were enrolled. The oscillatory reactivity to single‐pulse TMS of the frontal, sensorimotor and parietal cortex was measured using event‐related spectral perturbation of TMS‐EEG. The temporal–spatial properties of the oscillatory reactivity were illustrated through life time, decay gradients and accumulative power. In DOC patients, an oscillatory reactivity was observed to be temporally and spatially suppressed. TMS‐EEG of DOC patients showed that the oscillations did not travel as far in healthy controls, in terms of both temporal and spatial dimensions. Moreover, cortical theta reactivity was found to be a reliable indicator in distinguishing DOC versus healthy controls when TMS of the parietal region and in distinguishing MCS versus VS/UWS when TMS of the frontal region. Additionally, a positive correlation was observed between the Coma Recovery Scale‐Revised scores of the DOC patients and the cortical theta reactivity.ConclusionsThe findings revealed a breakdown of oscillatory effective networks in DOC patients, which has implications for the use of TMS‐EEG in DOC evaluation and offers a neural oscillation viewpoint on the neurological basis of human consciousness.
Negative formaldehyde release from textiles washed with a formaldehyde‐containing laundry soap according to manufacturer instructions: An application of chromotropic acid testing
Authors: Battis Nicholas , Ekstein Samuel F. , Shaik Javed A. , Hylwa Sara 
Published: September 2023

AbstractBackgroundFormaldehyde is a common preservative used to prevent microbial growth in water. It can be found in personal care products and household cleaning products, including laundry detergents. Formaldehyde has frequently been recognised as a cause of allergic contact dermatitis, but whether it remains present in textiles washed with formaldehyde‐containing laundry detergents is unknown.ObjectivesThis study aimed to utilise the chromotropic acid method (CAM) to assess formaldehyde release from textiles washed with a laundry detergent known to contain formaldehyde.Materials and MethodsTextiles were laundered with a detergent containing calcium formate at four concentrations (0×, 0.5×, 1× and 5× the recommended amount per manufacturer label) and kept wet or allowed to dry. Select textiles were subjected to an additional rinse cycle. Textiles were then tested utilising the CAM. A sample of the pure laundry detergent was also tested using the CAM.ResultsThe CAM was positive only for wet textiles washed at 5× the recommended concentration of detergent and pure detergent. All dry textiles were negative.ConclusionsFormaldehyde release was not detected from any textiles washed following the manufacturer's recommendations. Once dry, it is likely safe for formaldehyde‐allergic patients to wear textiles washed with formaldehyde‐containing detergents.
A disease model predicting placebo response and remission status of patients with ulcerative colitis using modified Mayo score
Authors: Moein Anita , Langenhorst Jurgen , Plan Elodie L. , Jin Jin Y. , Kågedal Matts , Kassir Nastya 
Published: September 2023

AbstractThe Mayo Clinical Score is used in clinical trials to describe the clinical status of patients with ulcerative colitis (UC). It comprises four subscores: rectal bleeding (RB), stool frequency (SF), physician's global assessment, and endoscopy (ENDO). According to recent US Food and Drug Administration guidelines (Ulcerative colitis: developing drugs for treatment, Guidance Document, https://www.fda.gov/regulatory‐information/s. 2022), clinical response and remission should be based on modified Mayo Score (mMS) relying on RB, SF, and ENDO. Typically, ENDO is performed at the beginning and end of each phase, whereas RB and SF are more frequently available. Item response theory (IRT) models allow the shared information to be used for prediction of all subscores at each observation time; therefore, it leverages information from RB and SF to predict ENDO. A UC disease IRT model was developed based on four etrolizumab phase III studies to describe the longitudinal mMS subscores, placebo response, and remission at the end of induction and maintenance. For each subscore, a bounded integer model was developed. The placebo response was characterized by a mono‐exponential function acting on all mMS subscores similarly. The final model reliably predicted longitudinal mMS data. In addition, remission was well‐predicted by the model, with only 5% overprediction at the end of induction and 3% underprediction at the end of maintenance. External evaluation of the final model using placebo arms from five different studies indicated adequate performance for both longitudinal mMS subscores and remission status. These results suggest utility of the current disease model for informed decision making in UC clinical development, such as assisting future clinical trial designs and evaluations.
“A cuff is not enough”: A community‐based participatory research approach to soliciting perspectives of African Americans with hypertension and their family members on self‐management intervention features
Authors: Woods Sarah B. , Udezi Victoria , Roberson Patricia N. E. , Arnold Elizabeth Mayfield , Nesbitt Shawna , Hiefner Angela 
Published: September 2023

AbstractWe aimed to solicit the perspectives of African Americans with hypertension and their family members on the desired features of a behavioral hypertension self‐management intervention. Using a community‐based participatory approach to intervention design, we conducted four dyadic focus groups, including African American community members with hypertension (n = 23) and their family members (n = 23), recruited from African American‐serving Christian churches in a large, southern metropolitan area. We used open‐ended questions to elicit participants' perspectives regarding program features they would recommend, intervention delivery, and barriers necessary to address. Our grounded theory analysis identified themes reflecting participants' recommendations for hypertension self‐management interventions to enhance health literacy and provide communication training via an accessible, population‐tailored, family‐based approach, which they believed has the potential to create family‐level impact on health across generations. Participants also recommended intervention researchers engage in advocacy (i.e., via physician education and policy change) as part of a broader impact on structural inequities driving worse hypertension and health outcomes for African Americans. The perceptions and recommendations of African Americans with a lived experience of hypertension, as well as their family members, aid in shaping acceptable and efficacious behavioral interventions aiming to promote hypertension self‐management behavior while leveraging the unique power of family relationships to create sustained behavior change.
Refuge‐yeah or refuge‐nah? Predicting locations of forest resistance and recruitment in a fiery world
Authors: Rodman Kyle C. , Davis Kimberley T. , Parks Sean A. , Chapman Teresa B. , Coop Jonathan D. , Iniguez Jose M. , Roccaforte John P. , Sánchez Meador Andrew J. , Springer Judith D. , Stevens‐Rumann Camille S. , Stoddard Michael T. , Waltz Amy E. M. , Wasserman Tzeidle N. 
Published: September 2023

AbstractClimate warming, land use change, and altered fire regimes are driving ecological transformations that can have critical effects on Earth's biota. Fire refugia—locations that are burned less frequently or severely than their surroundings—may act as sites of relative stability during this period of rapid change by being resistant to fire and supporting post‐fire recovery in adjacent areas. Because of their value to forest ecosystem persistence, there is an urgent need to anticipate where refugia are most likely to be found and where they align with environmental conditions that support post‐fire tree recruitment. Using biophysical predictors and patterns of burn severity from 1180 recent fire events, we mapped the locations of potential fire refugia across upland conifer forests in the southwestern United States (US) (99,428 km2 of forest area), a region that is highly vulnerable to fire‐driven transformation. We found that low pre‐fire forest cover, flat slopes or topographic concavities, moderate weather conditions, spring‐season burning, and areas affected by low‐ to moderate‐severity fire within the previous 15 years were most commonly associated with refugia. Based on current (i.e., 2021) conditions, we predicted that 67.6% and 18.1% of conifer forests in our study area would contain refugia under moderate and extreme fire weather, respectively. However, potential refugia were 36.4% (moderate weather) and 31.2% (extreme weather) more common across forests that experienced recent fires, supporting the increased use of prescribed and resource objective fires during moderate weather conditions to promote fire‐resistant landscapes. When overlaid with models of tree recruitment, 23.2% (moderate weather) and 6.4% (extreme weather) of forests were classified as refugia with a high potential to support post‐fire recruitment in the surrounding landscape. These locations may be disproportionately valuable for ecosystem sustainability, providing habitat for fire‐sensitive species and maintaining forest persistence in an increasingly fire‐prone world.
Method of raw materials selection for production of the MgO‐C bricks of comparable properties using PCA and K‐medoids
Authors: Sado Sebastian 
Published: September 2023

AbstractMgO‐C refractories are zonally installed in most of the key heat devices of steel and iron metallurgy with the exposure to various corrosive factors. One of the most significant factors is low‐temperature decarburization resulting in the oxidation of carbon in MgO‐C refractories and loss of its properties. Moreover MgO‐C materials are exposed for direct attack of liquid slag or steel melt which easily infiltrate the porous, decarburized hot face of the bricks. Cost of MgO‐C materials of high quality is important for refractories user. For this reason researches try to develop materials of high quality but with lower cost by substituting fused raw materials with sintered ones or with recycled MgO‐C. In this article, method of selecting the raw materials for production of MgO‐C bricks with comparable properties is shown. Twenty different variants of MgO‐C materials were prepared with the use of various quality raw materials. Basic properties of the industrially produced MgO‐C bricks were measured. To select recipes with similar quality principal components analysis method and K‐medoids algorithm were applied. To verify the results corrosion resistance of selected MgO‐C materials were tested with the use of induction furnace of medium frequency.
Open government, civic tech and digital platforms in Latin America: A governance study of Montevideo's urban app ‘Por Mi Barrio’
Authors: Aguerre Carolina , Bonina Carla 
Published: September 2023

AbstractDigital technologies have a recognised potential to build more efficient, credible, and innovative public institutions in Latin America. Despite progress, digital transformation in Latin American governments remains limited. In this work, we explore a peculiar yet largely understudied opportunity in the region: pursuing digital government transformation as a collaborative process between the government and civil society organisations. To do so, we draw from information systems research on digital government and platforms for development, complemented with governance theory from political science and conduct an interpretive in‐depth case study of an urban reporting platform in Montevideo called ‘Por Mi Barrio’. The study reveals three mutually reinforced orders of governance in the trajectory of the project and explain how the collaboration unfolded over time: (i) a technical decision to use open platform architectures; (ii) the negotiation of formal and informal rules to make the project thrive and (iii) a shared, long‐term ideology around the value of open technologies and technical sovereignty grounded in years of political history. Using a contextual explanation approach, our study helps to improve our understanding on the governance of collaborative digital government platforms in Latin America, with specific contributions to practice.
Optimisation models for project selection in asset management: an application to the water sector
Authors: Vilarinho Hermilio , Barbosa Flávia , Nóvoa Henriqueta , Silva Jaime Gabriel , Yamada Luciana , Camanho Ana S. 
Published: September 2023

AbstractA significant challenge in asset management is the selection of investment projects for infrastructures, which often relies on subjective judgement and lacks structured decision support methods. This challenge is particularly complex in water systems due to the diverse and heterogeneous nature of the components requiring investment. While the infrastructure value index (IVI) is widely used to characterise assets and support investment decisions in the water sector, its application in optimisation models for generating efficient project portfolios remains unexplored. To address this research gap, this study introduces optimisation models for generating investment portfolio plans in water systems' asset management. The proposed approach includes two mixed‐integer linear programming (MILP) models that determine optimal solutions and an evolutionary algorithm that offers sub‐optimal alternative investment selection plans to provide decision‐makers with additional choices for balancing optimal outcomes. The primary contribution of this research is the combined utilisation of MILP and evolutionary algorithms, integrating the IVI into the decision‐making process. These tools provide decision‐makers with structured methods for defining investment plans and minimising the subjective elements typically associated with such processes. To illustrate the effectiveness of the models, a case study is presented involving a pumping station of a Portuguese water company. The results demonstrate the practical application and benefits of the proposed approach in optimising investment decisions. This research contributes to advancing asset management practices by integrating quantitative optimisation techniques and leveraging the IVI, thereby enhancing the objectivity and efficiency of investment planning in water systems' asset management.
Fabrication of Cu/ZnO‐loaded chitosan hydrogel for an effective wound dressing material to advanced wound care and healing efficiency after caesarean section surgery
Authors: Wang Qiaoying , Li Qingqing , Zhu Lingping , Lin Chenxiao , Chen Qiaoling , Chen Hong 
Published: September 2023

AbstractWound infections and delayed complications after caesarean section surgical procedure to mothers would have a prevalence of discomfort, stress and dissatisfaction in the postpartum period. In this report, one‐pot synthesis is used for the preparation of chitosan (CS)‐based copper nanoparticles (nCu), which was used for the preparation of zinc oxide (ZnO) hydrogel as wound dressing materials after surgery. The antibacterial activity of (CS‐nCu/ZnO) developed hydrogels was studied zone of inhibition, against gram‐positive and gram‐negative bacteria. The antibacterial activity of the CS‐nCu/ZnO hydrogel demonstrated that nanoformulated hydrogel materials have provided excellent bactericidal action against clinically approved bacterial pathogens. The biocompatibility and in vitro wound healing potential of the developed wound closure materials were studied by MTT assay and wound scratch assay methods, respectively. The MTT assay and cell migration assay results demonstrated that CS‐nCu/ZnO hydrogel material induces cell compatibility and effective cell proliferation ability. These findings suggest that the CS‐nCu/ZnO hydrogel outperforms CS‐ZnO in terms of wound healing and could be used as a wound closure material in caesarean section wound treatment.
RETRACTED: Diabetic foot wound ulcers management by vacuum sealing drainage: A meta‐analysis
Authors: Yang Liuqing , Zhao Ning , Yang Min , Huang Jiao , Fu Xiaomin , Lei Changjiang , Cai Pengzhu 
Published: September 2023

AbstractThe meta‐analysis aimed to assess and compare diabetic foot wound ulcer management by vacuum sealing drainage. Using dichotomous or contentious random‐ or fixed‐effects models, the outcomes of this meta‐analysis were examined, and the odds ratio (OR) and the mean difference (MD) with 95% confidence intervals (CIs) were computed. Twenty‐three examinations from 2000 to 2023 were enrolled for the present meta‐analysis, including 1928 individuals with diabetic foot ulcers. Vacuum sealing drainage had significantly lower wound healing (OR, 2.35; 95% CI, 1.79–3.08, p < 0.001), lower duration of therapy (MD, −6.19; 95% CI, −10.06 to −2.32, p = 0.002), higher wound size reduction (MD, 4.22; 95% CI, 0.87–7.56, p = 0.01) and lower complication (OR, 0.32; 95% CI, 0.13–0.80, p = 0.01) compared with standard therapy in patients with diabetic foot ulcers. The examined data revealed that vacuum sealing drainage had significantly lower wound healing, duration of therapy and complication rates, as well as higher wound size reduction, compared with standard therapy in patients with diabetic foot ulcers. Yet, attention should be paid to its values since most of the selected examinations had a low sample size.