bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and morphological features of bladder cancer course in HPV-infected patients
Authors: Pulatova A. A. , Dimitriadi S. N. , Kutilin D. S. , Zykova T. A. , Frantsiyants E. M. , Shevyakova E. A. , Hwan V. K. , Goncharov S. I. 
Published: September 2023

Purpose of the study. To study the histological type, grade of tumor differentiation in patients with primary and recurrent clinically non-muscle-invasive bladder cancer (NMIBC) with highly carcinogenic human papillomavirus (HPV) infection.Patients and methods. Formalin-fixed and paraffin-embedded bladder tumor tissue samples have been studied in 159 patients who underwent transurethral resection (TUR) of the bladder, for the presence of HPV DNA. To detect, quantify and differentiate DNA of HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 genotypes in the samples, the AmpliSense® HPV HRC genotype-titer-FL was used. The result of the study was taken into account when the amount of DNA of the β-globin gene was at least 1000 copies per reaction. In order to statistically analyze our data we used the Fisher exact test and also calculated the odds ratio (OR) and 95 % CI.Results. According to the results of the study, out of 159 patients, high-risk HPV DNA was detected in the tumor tissue in 59 (37.1 %), of which HPV type 16 was found in 52 patients (89.4 %), HPV 18 was detected in 4 patients type (6.7 %) and type 35 in 3 (5.08 %). In a morphological study of the tissues of HPV-positive patients, the grade of tumor differentiation was G2 in 18 cases (30.5 %), G3 in 37 blocks, and G1 was detected only in 4 cases (6.7 %). In the presence of HPV, the chance of detecting a stage G3 tumor increases by 4.3 times. According to the received data, we can assume that there is a close relationship between detection in HPV patients of high-risk genotypes with moderately differentiated and low-differentiated forms of bladder cancer.Conclusion. this study may indicate that HPV infection affects the grade of tumor differentiation, and this, in turn, may allow the use of the HPV test to assess the nature of the development of relapse and/or progression of the disease.
Prospects of differential diagnosis of focal lesion of pancreas by the microRNA assessment
Authors: Kniazeva M. S. , Shestopalova T. M. , Zabegina L. M. , Shalaev A. V. , Ratnikova A. K. , Kashchenko V. A. , Vorobyev S. L. , Malek A. V. 
Published: September 2023

Purpose of the study. Identification of potential miRNA markers in material of focal pancreatic lesions.Materials and methods. Samples of focal pancreatic lesions after histological evaluation were enrolled in the study including chronic pancreatitis (ChP) (n = 23), low-grade pancreatic intraepithelial neoplasia /PanIN‑1/2 (n = 19), high-grade pancreatic intraepithelial neoplasia /PanIN‑3 (n = 8), and invasive pancreatic ductal adenocarcinoma PDAC (n = 26). Workflow of research included the profiling of cancer-associated miRNA in pooled samples, the selection of potential marker miRNAs, the assessment of selected miRNAs expression in total collection of specimens, the identification of differentially expressed miRNAs, and the approbation of new algorithm of data interpretation via ratio of “reciprocal miRNA pair”. Consequent reactions of revers transcription and quantitative teal-time PCR were used.Results. The expression levels of miR‑216a and miR‑217 were decreased in the following order: PanIN‑1/2 > PanIN‑3 > PDAC. Moreover, miR‑375 was up-regulated while miR‑143 was down-regulated in the PDAC. Differential diagnostics of PDAC versus focal chronic pancreatitis might be performed with high accuracy (AUC > 0.95) by assessment panel of four molecules: miR‑216a, miR‑217, miR‑1246 and Let‑7a.Conclusion. The assessment of microRNAs in pancreatic lesions is a promising approach for the differential diagnosis of PDAC, but this technology requires further validation with an increase in the number of samples.
Gastric cancer modeling in immunodeficient mice with orthotopic xenotransplantation
Authors: Kurbanova L. Z. , Karasev T. S. , Goncharova A. S. , Kolesnikov E. N. , Maksimov A. Yu. , Averkin M. A. , Galina A. V. , Romanova M. V. , Gusareva M. A. , Zinkovich M. S. 
Published: September 2023

Purpose of the study. Creation of a transplantable orthotopic PDX model of gastric cancer in Balb/c Nude immunodeficient mice using implantation and injection.Materials and methods. Two methods, that are injection and implantation, were used to create an orthotopic PDX model of human gastric cancer. The first method involved injections of a suspension of a mechanically disaggregated patient's tumor after filtration into the gastric wall of Balb/c Nude mice. For the second method, small fragments (3 × 3 × 3 mm) of patients' tumors were implanted in the gastric wall of mice along the greater curvature with a dissection of the serous muscular layer.Results. Control laparotomy in Balb/c Nude immunodeficient mice showed a successful engraftment of the tumor material at the 1st and 3rd procedures when using the implantation method for the creation of a PDX model of gastric cancer. The injection method was ineffective, and no models were created. The histological type of the obtained PDX models was compared to the type of the donor tumor by histological examination (hematoxylin and eosin staining). The tumor grade remained stable and did not change during xenograft passage, which showed that the obtained model was identical to the histotype of the donor tumor.Conclusion. The presented implantation method for the model creation results in effective tumor engraftment. The developed model can be used to test the effectiveness of anticancer or antimetastatic drugs, for studying the functions of biomarkers, or in assessing the microenvironment of a gastric cancer.
A rare clinical case of syringomyelia progression in the presence of Chiari I malformation following the surgery
Authors: Rostorguev E. E. , Kuznetsova N. S. , Maslov A. A. , Hatyushin V. E. , Matevosyan B. V. , Reznik G. A. , Pandova O. V. , Shalashnaya E. V. 
Published: September 2023

Today, an Arnold-Chiari malformation is defined as a developmental pathology of the craniovertebral junction manifested by a discrepancy between the volume and contents of the posterior cranial fossa, which in turn leads to compression of neurological structures and changes in the cerebrospinal fluid circulation. There are several theories of the correlation between Chiari malformation and syringomyelia, but the exact mechanism of syringomyelia development remains unclear.This article describes a clinical case of treatment of a child with Chiari I malformation and syringomyelia within the cervical and thoracic segments of the spinal cord; after complete posterior fossa decompression, syringomyelia progressed in the early postoperative period with the development of a severe neurological deficiency. Since there is no standard treatment of such postoperative complications, a decision was made on the expectant management of the patient. From the twentieth day of the postoperative period, the patient showed complete regression of the neurological deficiency and positive MRI dynamics of syringomyelia.The presented clinical case raises such issues as not only the pathophysiology of syringomyelia progression after complete posterior fossa decompression, but also the determination of patient management tactics in case of a complicated postoperative course of the disease.The presented clinical case is of interest due to the rarely described aggravation of syringomyelia with enhancing neurological symptoms in the early postoperative period after complete posterior fossa decompression. Our observation suggests that the expectant management of the patient, despite syringomyelia progression with neurological deficiency aggravation after posterior fossa decompression, allowed a favorable long-term outcome of Chiari I malformation.
La consulta previa desde el desarrollo jurisprudencial de la Corte Constitucional del Ecuador. (Sentencias Nº 001 – 10 – SIN – CC, Nº 38-13-IS/19 Y Nº 273-19-JP/22)
Authors: Cedeño Pincay. Irvin Ramón 
Published: September 2023

La consulta previa es un derecho colectivo reconocido en la Constitución del Ecuador a través de la que se busca garantizar la participación de los miembros de las comunidades indígenas en decisiones relativas a sus territorios. El objetivo del estudio es analizar jurisprudencia sobre la consulta previa emitida por la Corte Constitucional del Ecuador, en su desarrollo se analiza doctrina relativa al derecho a la consulta previa, se fundamenta jurídicamente el derecho a la consulta previa de acuerdo con la normativa vigente y se exponen argumentos basados en la jurisprudencia emitida por la Corte Constitucional del Ecuador sobre la consulta previa. Se desarrolla mediante una metodología jurídico-descriptiva, cualitativa, se consideran instrumentos de derecho internacional y sentencias de la Corte Interamericana de Derechos Humano y sentencias de la Corte Constitucional del Ecuador. Se concluye que la Corte Constitucional del Ecuador a través de la jurisprudencia ha buscado dotar de contenido a la consulta previa, que la falta de una legislación secundaria limita el ejercicio de este derecho esto refleja el escaso interés que desde el órgano legislativo ha existido frente a la regulación de este derecho. Por su parte la Corte Constitucional a través de las sentencias Nº 001 – 10 – SIN – CC, Nº 38-13-IS/19 y Nº 273-19-JP/22 ha dotado de contenido al derecho a la consulta previa en observancia con parámetros establecidos en instrumentos de derecho internacional y sentencias expedidas por la Corte Interamericana de Derechos Humanos.
The Effect of an Intensive Hand Exercise Program on Muscle Strength and Hand Functions in Patients with Rheumatoid Arthritis
Authors: ARSLAN Hakkı , ERDEM İbrahim Halil 
Published: September 2023

Aim: This study investigated the effectiveness of classic and intensive exercise programs in patients with rheumatoid arthritis (RA) and their relationship with disease activity and patient functional well-being.
Material and Methods: Sixty patients aged 18 to 65, diagnosed with RA in our clinic, were randomly divided into two groups of 30 each. The first group received an intensive exercise program, while the second group received a classic exercise program. Disease activity was evaluated using erythrocyte sedimentation rate, C-reactive protein, DAS28Sedim, and DAS28Crp. Daily life activities were assessed using the HAQ score and Duruöz hand index. Joint mobility measurements were taken with a goniometer, and muscle strength measurements were taken using a manual dynamometer and pinch meter. Hand and wrist radiographs were taken and evaluated according to Steinbroker stages before exercise therapy.
Results: Improvement was observed in both groups' Visual Analog Scale (VAS) and Health Assessment Questionnaire (HAQ) scores, with a significant improvement in VAS scores favoring the intensive exercise group.
Conclusions: The study concluded that exercise therapy had positive effects on patients' hand functions, and the intensive exercise program was more effective.
Relationship Between Chronic Spontaneous Urticaria and Fibromyalgia Syndrome
Authors: KULAKLI Sevgi , OĞUZ Işıl Deniz , SARI İlker Fatih , ÖĞÜT Halil 
Published: September 2023

Aim: Autoimmunity, peripheral nerve dysfunction, and neurogenic inflammation are common mechanisms in chronic spontaneous urticaria (CSU) and fibromyalgia syndrome (FMS). We aimed to detect the prevalence of FMS in patients with CSU and to determine whether this prevalence was affected by the severity of urticaria, and dermatology life quality.
Material and Methods: Fifty-three patients with CSU and 49 controls were enrolled in this prospective, controlled, cross-sectional study. The severity of CSU was assessed using Urticaria Activity Scores (UAS), and Dermatology Life Quality Index (DLQI) scores were recorded. The 2016 fibromyalgia diagnostic criteria were used for the diagnosis of FMS, and FMS-related functional disability was assessed using the Fibromyalgia Impact Questionnaire (FIQ).
Results: Fibromiyalgia prevalence and the FIQ scores were higher in the CSU group than in the control (p=0.033 and p=0.004, respectively). There was no statistically significant difference between the urticaria durations and UAS of CSU with and without FMS (p>0.05), but DLQI scores were statistically significantly higher in CSU with FMS (p=0.007). A statistically significant moderate positive correlation was present between DLQI and FIQ, Widespread Pain Index, and Symptom Severity Scale scores (r=0.500, r=0.408, r=0.469, r=0.507, respectively).
Conclusions: The prevalence of FMS and the disability due to FMS was increased in CSU. Furthermore, the FMS prevalence was not affected by the duration and severity of urticaria; however, it was associated with decreased quality of life.
Analysis of Google Trends for Viral Hepatitis A, B, C, D, and E
Authors: SEMET Cihan 
Published: September 2023

Aim: Our main objective in this study was to rigorously measure global interest and awareness of viral hepatitis through a systematic evaluation of data collected from Google Trends.
Material and Methods: We compiled and categorized the Search Volume Index (SVI), a quantitative measure covering the global regional distribution associated with the search terms "Hepatitis A", "Hepatitis B", "Hepatitis C", "Hepatitis D" and "Hepatitis E" over a period of approximately ten years from 2013 to 2022.
Results: According to our analysis, there has been a noticeable increase in interest in Hepatitis A and B, while interest in Hepatitis C has declined after peaking in 2015. Meanwhile, interest in Hepatitis D and E continued to show a very low profile. Our rigorous research found that Guatemala recorded the highest rate of interest for Hepatitis A, Ghana for Hepatitis B, Pakistan for Hepatitis C, Kyrgyzstan for Hepatitis D and Namibia for Hepatitis E. Conclusions: This study highlights the potential for using tools such as Google Trends in organizing public health monitoring and awareness campaigns.
Characterization of water quality and the effects of land use and seasonality on springs in eastern Amazonia
Authors: Nascimento Rodrigo Oliveira do , Araújo Suzana Romeiro , Sodré Silvana do Socorro Veloso , Freitas Kemuel Maciel , Alves Aline Chaves 
Published: September 2023

Land-use changes followed by inadequate management may cause serious impacts to springs, generating losses in quality and availability. An alternative to minimize or mitigate potential future impacts is to monitor the water quality parameters of micro-watersheds in the medium- and long-term. This monitoring is essential for planning purposes and governmental environmental regulation, especially in highly altered regions in the Amazon, such as Paragominas, in the state of Pará. This study investigated the influence of land use change and seasonality on the quality of springs. For this purpose, physicochemical parameters characteristic of water quality in five collection points of springs with a surrounding area of distinct land use history were analyzed between 2015 and 2017. Following the current legislation, the only parameter in imbalance was dissolved oxygen (DO). However, these are common values, considering springs. The results showed that most of the parameters presented variation to different land uses. This interpretation was intensified mainly by the variations in Sodium, TN, DO and temperature. However, few of these variables were related to local seasonality (only turbidity, sulfate and potassium). These results prove that it is possible to integrate the change in the use and occupation of the basin, determined by the variations observed in the sampled points. Thus, studies and diagnostics that can subsidize management in basin areas are an important tool to direct public policies to improve environmental and social quality for the population living around these basins.
Keywords: Amazon, land use change, springs, water quality.
Screening of some Brazilian fungi and bacteria able to decolor a textile effluent and evaluation of effluent toxicity on Artemia salina
Authors: Oliveira Jackelly Felipe de , Coelho Luana Cassandra Breitenbach Barroso , Silva Leonor Alves de Oliveira da , Gusmão Norma Buarque de 
Published: September 2023

The screening of organisms capable of decoloring a textile effluent was performed using 4 fungi (F24= Aspergillus spp.., F48= Aspergillus spp.., F98= Aspergillus fumigatus, FTL01=Trametes lactinea) and 2 bacteria (T9 = Bacillus subtilis, T19 = Alcaligenes faecalis). These organisms were submitted to effluent decoloration assays in which the T. lactinea fungus demonstrated the greatest decoloration capacity (51.35%); thus, it was selected as a model organism for this study. The effect of pH, temperature and carbon sources on the effluent decoloration rate by T. lactinea was studied. The effluent decoloration by T. lactinea reached levels higher than 89.77%. Finally, acute toxicity assays on Artemia salina revealed that T. lactinea treatment reduced the toxicity of raw effluent.
Keywords: biodegradation, textile dyes, water treatment.
Comparison of Xanthine Oxidase Inhibitory Effect of Swertia chirayita and Febuxostat in Vitro
Authors: Qura-Tul-Ain  , Sidra Mushtaq  , Naveeda Manzoor  , Mahreen Akhtar  , Anila Errum  , Naseem Saud Ahmad  
Published: September 2023

Introduction:Plasma uric acid ? 6.5 to 7.0 mg/dl increases the incidence of gout, hypertension, diabetes mellitus type 2, stroke and heart failure. Low purine diet, use of xanthine oxidase inhibitors allopurinol and febuxostat; or uricosuric agent’s probenecid, sulfinpyrazone and Benzbromarone are being used for treatment. Swertia chirayita extract (SCE) being practiced as anti-inflammatory and anti-oxidant was evaluated for inhibition of xanthine oxidase.
Aims & Objectives: To observe the in vitro effect of Swertia chirayita ethanolic extract on xanthine oxidase inhibition and its comparison with medicine febuxostat.
Place and duration of study: The study was conducted in Pharmacology Department, University of Health Sciences Lahore during June-August 2017.
Material & Methods: Xanthine oxidase (XO) inhibition by ethanolic extract of the herb was compared with febuxostat. Serial dilutions of test compounds ranging from 1.25µg/ml to 100 µg/ml were prepared in absolute ethanol. Fixed volumes of xanthine, xanthine oxidase, phosphate buffer and test compounds were incubated. Uric acid concentration in the reaction mixture was calculated by measuring absorbance at 295nm by using Ultraviolet- Visible Spectrophotometer. Mean values and percent inhibition (± SD) was calculated. EZ Fit Enzyme Kinetic program was also used to calculate inhibitory concentration 50 percent (IC50), Michaelis constant (Km) and maximum rate of reaction (Vmax).
Results: At maximum dose, (100 µg/ml) xanthine oxidase inhibition by febuxostat and Swertia chirayita was 98% and 80% percent respectively (p=0.01). Swertia chirayita extract showed IC50 at 9.15± 1.2 µg/ml whereas IC50 of febuxostat was 7.91± 0.9 µg/ml.
Conclusion: In the light of XO inhibition, Swertia chirayita has potential to be explored as antihyperuricemic agent.
High Body Mass Index a Risk Factor of Preeclampsia: A Hospital Based Case-Control Study
Authors: Zoofishan Qureshi  , Muhammad Ashraf Chaudhry  , Nayyer Uzair  , Tariq Mukhtar Farani  , Uzair Mumtaz  , Muhammad Umer Farooq  
Published: September 2023

Introduction: Preeclampsia is a disease which is associated with pregnancy and enhances perinatal and maternal morbidity and mortality. The relationship of obesity and preeclampsia is still under research among different populations.
Aims & Objectives: The objective of this study is to determine the strength of association of body mass index with preeclampsia in pregnant women.
Place and duration of study: Gynecology/Obstetric Departments of Services Hospital, Lahore General Hospital and Sir Ganga Ram Hospital Lahore from 1st January 2019 to 30th June 2019.
Material & Methods: It was a matched case-control study. Sixty six diagnosed pre-eclamptic antenatal women were selected as cases and they were matched with equal number of normotensive antenatal women. The definition, source and eligibility criteria of cases and controls were pre-determined. Odds ratio was calculated to measure strength of association between preeclampsia and body mass index (BMI).
Results: Among 66 preeclamptic women, the mean age was 26.76+5.82 years and among 66 normotensives mean age was 25.36+4.69 years. Among cases, 49.7% had BMI >25 and 30.3% had BMI <25. Likewise among controls, 40.9% had BMI >25 and 59.1% had BMI <25. Odds Ratio was 3.32. The result was found statistically significant (P=0.001).
Conclusion: Study concluded that high body mass index is a risk factor of preeclampsia among pregnant women.
Effectiveness of Amnion on Chronic Wounds Vs Saline Dressing
Authors: Muhammad Ali Rafique Mirza  , Ammara Mazhar  , Waheed Bhutto  , Abdul Saeed Khan  
Published: September 2023

Introduction: Chronic wounds have deleterious effects on patient health and quality of life. Human placental membranes consist of amnion and chorion which are conjoined and avascular structures. These membranes have abundant growth factors and cytokines and extracellular matrix which can induce angiogenesis and dermal fibroblast proliferation and recruit mesenchymal stem cells involved in wound repair supplying an ideal environment for wound closure.
Aims & Objectives: To compare the effectiveness of human amnion vs. normal saline dressing in chronic wounds.
Place and duration of study: This study was conducted at Shaikh Zayed Medical Complex for a period of 6 months.
Material & Methods: This randomized control trial was carried out in the Department of Plastic Surgery at Shaikh Zayed Hospital, Lahore from 20-08-2019 to 20-02-2020. 60 patients of both genders,18 - 65 years of age having ulcers of more than six weeks duration and larger than 10 x 10 cm were inducted using non -probability consecutive sampling technique. They were divided into two groups of 30 patients each. Group A (Amnion) and Group B (Saline Dressing). Patients were assessed pre-treatment, wound dressings applied daily and followed up for 6 weeks for efficacy of dressing based on reduction in wound size and its relation to wound chronicity. Data was entered and analyzed using SPSS version 20.
Results: There were 27 (90%) male and only 3 (10%) females patients in group A and 21 (70%) male and 9 (30%) females patients in group B. Mean age of the patients was 45.60±12.51 years for group A and 44.20±11.65 years for group B. 27 (90%) patients demonstrated good efficacy of treatment at six weeks in group A as compared to 5 (17%) patients in group B which is statistically significant (P 0.041). Significant healing was noted in amnion treated chronic wounds of up to 21 weeks duration.
Conclusion: Our randomized control trial shows that amniotic membrane dressing are beneficial in the treatment of chronic wounds as compared to normal saline dressings.