bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of temperature on antioxidant enzyme activities and gene expression in sand worm Perinereis quatrefagesi (Grube, 1878)
Authors:   , Suksai Sucharat 
Published: September 2023

Polychaetes is one of intertidal animals that face the fluctuations of coastal environment through time. One of important environmental factors to animals is temperature regarding to the fluctuation of temperature in intertidal environment. Human activities could also cause high changes in water temperature by high temperature discharges from urban and industries. Since global warming is still a main issue, an increase of seawater temperature about 3°C could be possible as a prediction. This study aimed to investigate effect of temperature in polychaetes Perinereis quatrefagesi at cellular level, which could be indicated by heat shock response and oxidative stress due to function of basic stress. Responses in the worms at the cellular level were measured using real-time PCR for oxidative stress gene expression and using enzyme assay for protein activities. Different temperatures (27, 29, 31, 33°C) with three replication and different exposure times (0 d, 12 hrs, 1 d, 3 d, 7 d, 14 d, and 28 d) were designed. Results of growth parameters showed a significant change in the final wet weight regarding the different temperature levels. While survival rates and specific growth rates gave a similar pattern with a significant difference at the highest temperature. This growth parameters, therefore, revealed the optimal temperature for culture this species at 29°C, while 27°C and 31°C were also acceptable with regard to the high survival rate. For gene expression, the highest upregulation of hsp70, hsp90, sod, and catalase expressed at 33°C. High rate of upregulation was found at the first 12 hours on all gene, except sod which was non-detectable. An increasing trend of hsp70 with temperatures was a significant difference at the first 12 hours exposure. For enzyme activity, SOD activity showed an increasing trend at the first 12 hours exposure and a decreasing trend at 12 hours later, while the highest peak of SOD activity was found at the end of the experiment for all treatment. The CAT activity demonstrated a significant rise in the first 12 hours. Overall, this study supports the negative effect of high temperatures on aquatic organism in term of significantly low growth parameter and active stress responses; heat shock proteins and antioxidant enzymes, especially at the first 12-24 hours exposure.
The severity of cryptococcus neoformans infection in Fc gamma receptor IIb deficient mice
Authors:   , Surawut Saowapha 
Published: September 2023

Cryptococcus neoformans, an encapsulated yeast commonly infecting the central nervous system, refers to as cryptococcal meningitis or cryptococcosis is commonly found in Thailand. C. neoformans is able to replicate, survive inside host macrophages, evade from the cells and use macrophages as a vehicle to disseminate to the target organs, a mechanism known as Trojan horse. Therefore, the containment of C. neoformans in phagocytic cell is important for host protective immunity. Cryptococcosis is common in immunocompromised host such as in patients with low CD4+ T helper cells in HIV-infection and patients taking immunosuppressive drugs. However, the incidence of cryptococcosis in healthy individual (HIV-uninfected) is increasing. Interestingly, it was reported that dysfunctional FcγRIIb polymorphisms are associated with cryptococcosis susceptibility. FcγRIIb is the only inhibitory receptor among the family of FcγRs. FcγRIIb activation leads to suppress of phagocytic activity and reduces cytokine production. Because macrophage is important immune cells against cryptococcosis, we hypothesized that dysfunctional FcγRIIb may influence cryptococcosis susceptibility through the alteration of macrophages functions. We investigated cryptococcosis in the FcγRIIb-/- mouse. Mortality after intravenous C. neoformans-induced cryptococcosis FcγRIIb-/- mice was higher than in age-matched wild-types. Severe cryptococcosis in the FcγRIIb-/- mice was demonstrated by high fungal burdens in the internal organs with histological cryptococcoma-like lesions and high levels of TNF-α and IL-6, but not IL-10. Interestingly, FcγRIIb-/- macrophages demonstrated more prominent phagocytosis but did not differ in killing activity in vitro or the production of TNF-α, IL-6 and IL-10 levels, compared to wild-type cells. Indeed, in vivo macrophage depletion with liposomal clodronate attenuated the fungal burdens in FcγRIIb-/- mice, but not wild-type mice. Upon transferring to wild-type mice, FcγRIIb-/- macrophages with phagocytosed Cryptococcus resulted in higher fungal burdens than FcγRIIb+/+ macrophages with phagocytosed Cryptococcus. Moreover, the T cell subpopulation analysis and IFN-γ intracellular staining suggested that cryptococcosis severity in FcγRIIb-/- mice did not cause by T helper cell defect. These results support, at least in part, a model whereby, in FcγRIIb-/- mice, enhanced C. neoformans transmigration occurs through infected macrophages. In summary, prominent phagocytosis, with limited effective killing activity, and high pro-inflammatory cytokine production by FcγRIIb-/- macrophages were correlated with more severe cryptococcosis in FcγRIIb-/- mice. In clinical translation, we propose FcγRIIb loss-of-function- polymorphisms as a new risk factor for cryptococcosis.
Effect Of Chromium Content On Heat Treatment Behavior And Abrasive Wear Resistance Of Multi-Alloyed White Cast Iron
Authors:   , Opapaiboon Jatupon 
Published: September 2023

The effect of Cr content on heat treatment behavior and abrasive wear resistance of multi-alloyed white cast irons with basic composition was investigated. The cast irons with varying Cr content from 3-9% were prepared. The annealed specimens were hardened from 1323K and 1373K austenitizing and then, tempered at 673K to 873K with 50K intervals. The microstructure of each specimen consisted of primary austenite dendrite (γP) and (γ+MC) and (γ+M2C) eutectics in specimens with 3 and 5%Cr. By contrast, (γ+M7C3) eutectic appeared in specimens with 6%Cr and more. The matrix in as-cast state was mostly retained austenite but that in as-hardened specimens composed of precipitated secondary carbide, martensite and retained austenite.

As for heat treatment behavior, hardness in as-hardened state increased to highest value at 5%Cr and subsequently, decreased as Cr content rose. The volume fraction of retained austenite (Vg) displayed similar trend to the hardness. The higher austenitizing temperature contributed to lower the hardness but increase the Vg values. In the tempered state, the hardness showed more or less secondary hardening depending on austenitizing temperature and Cr content. The degree of secondary hardening was greater in the specimen hardened from higher austenitizing temperature. The maximum tempered hardness (HTmax) was obtained at 773K to 798K tempering and high austenitizing temperature offered higher HTmax. The Vg decreased gradually as the tempering temperature was elevated. The Vg value at HTmax was less than 5% in all the specimens. The highest value of HTmax was obtained in 5%Cr specimen regardless of austenitizing temperature.

In order to evaluate the abrasive wear resistance, the as-hardened (As-H), HTmax, lower and higher than HTmax (L-HTmax and H- HTmax) specimens were evaluated using Suga abrasion test and rubber wheel abrasion test. A linear relation between wear loss (Wl) and wear distance (Wd) was obtained in all the specimens. The wear rate (Rw) varied depending on heat treatment condition and Cr content. The lowest Rw value or highest wear resistance was obtained in the As-H specimen followed by HTmax specimen. The highest Rw value or lowest abrasive wear resistance was obtained in L-HTmax or H-HTmax specimen, regardless of Cr content and austenitizing temperature. In both abrasive wear tests, the Rw decreased continuously until 6%Cr and after that, it increased gradually with a rise of Cr content. The high austenitizing temperature provided lower Rw value than low austenitizing temperature in all range of Cr content. The Rw decreased with an increase in hardness. In the region of Vg less than 5%, the Rw values scattered in a wide range depending on the hardness. When the Vg got over 5%, the Rw value decreased continuously increasing of Vg value. The worn surface consisted of grooving, scratching and pitting in all the specimens. The pitting was found in eutectic area while the grooving and scratching were observed in matrix.
Preparation and characterization of nickel based nanocomposite coatings with enhanced mechanical and surface properties
Authors:   , Das Malay 
Published: September 2023

Ni-W alloy and Ni-W/diamond monolayer and multilayer composite coatings were prepared by electrodeposition from a Ni-W plating bath with diamond particles suspended into the bath. Ni-W/diamond composite coatings were also prepared with varying amounts of Cobalt sulphate additives. The morphology and the composition of the deposits was investigated by means of SEM and EDS, respectively. The effect of operating conditions and additives on the Ni-W coatings were analyzed. The effect of the plating parameters, composite particle size, multilayer deposits and cobalt additives on microstructure and mechanical properties of the Ni-W/diamond composite coatings was investigated. The electrodeposited Ni-W/diamond deposits reported a maximum hardness of 1207 ± 32 Hv whereas, the maximum hardness reported by the Ni-W alloy coatings was recorded to be less than 750 Hv which underlines the significant impact the diamond particles have on enhancing the hardness of the base Ni-W matrix. The addition of Cobalt sulphate in the plating bath in smaller quantities tends to enhance the hardness and wear resistance of the Ni-W/diamond composite coatings and this trend is reversed as the amount of cobalt sulphate in the plating bath is increased beyond 0.1 g/L.
Comparative study of the accuracy of single tooth implants placed between static and mental computer assisted surgery
Authors:   , Smitkarn Palita 
Published: September 2023

Objective: This randomized controlled clinical trial aimed to compared the accuracy of implant position between static and mental Computer Assisted Implant Surgery (CAIS) in single tooth gap.

Materials and methods: 52 patients who received 60 implants in single tooth gap, randomized equally into static or mental CAIS. In static CAIS group, data from CBCT scan and data from intraoral or model scan were utilised for the three dimensional virtual planning implant positioning and desiring of a three dimensional surgical guide in implant planning software. Implant bed preparation and implant insertion were done through the three dimensional surgical guide. Implants in the mental CAIS group were virtually planned in the same software, but surgery was done in freehand manner. After the surgery, postoperative CBCT was superimposed onto preoperative CBCT. Deviation in angle, implant shoulder and apex between planned and final implant positions were measured and compared in the same software.

Results: In static CAIS group, mean angle deviation, 3D deviation at implant shoulder and implant apex were 3.1 ± 2.3°, 1.0 ± 0.6 mm and 1.3 ± 0.6 mm, respectively. In mental CAIS group, mean angle deviation, 3D deviation at implant shoulder and implant apex were 6.9 ± 4.4°, 1.5 ± 0.7 mm and 2.1 ± 1.0 mm respectively. Statistically significant differences were found in all dimensions between two groups.

Conclusion: Static CAIS demonstrated higher accuracy of implant positioning compared to the mental CAIS in single tooth gap.
Production and characterization of cutinase from tropical fungi and application with detergent for stain and fuzz removal from spun polyester fabrics
Authors:   , Sooksai Taweeporn 
Published: September 2023

Fungi were isolated from agricultural soil and plant specimens. Twenty three isolates from 44 fungal isolates were found to produce cutinase by using PCL-plate clearing assay and their cutinase activity were investigated. All the cutinase-producing fungi were cultured in liquid mineral medium (LMM) containing PCL or crude cutin extracted from papaya peels, which was a carbon source for cutinase production. Cutinase activity was assayed base on the hydrolysis of paranitrophenyl butarate (p-NPB). It was found that PBURU-T5 gave the highest cutinase activity. The isolate PBURU-T5 was selected for identification based on morphology together with comparative nucleotide sequencing from internal transcribed spacer region of the ribosomal RNA gene (rDNA-ITS) and it was identified as Fusarium falciforme. The optimum conditions for the production of cutinase from F. falciforme PBURU-T5, showed that the culture medium with initial pH at 7.0, incubation temperature 30 °C at 150 rpm with the optimal medium composition consisting of 2.5 g/L cutin extract from papaya peels as a carbon source, 4 g/L peptone as nitrogen source by harvesting on 4 days of incubation provided the highest cutinase activity of 4±0.15 U/ml. The crude cutinase showed the highest enzyme activity at pH 9.0 and temperature 35 °C. For the detergent application, cutinase enzyme (powder form) from F. falciforme PBURU-T5 was mixed with a commercial detergent and was used for oily stain and pill/fuzz removal from spun-polyester fabrics through laundry. Then, the fabric was measured for color values, analyzed for pilling rate, and tested for weight loss and bursting strength loss. When the weight ratio of detergent: enzyme at 1:0.1 was used for fabric washing, oily stain could be completely removed in one washing cycle while fuzzy hair and pill was removed to the acceptable pilling rate of 3-4 (from pilling rate of 1 before washing) in eight washing cycles. After washing, fabric lost 2.68 % of its weight and 8 % of its bursting strength which was not a big concern for polyester wearers.
Improved barrier properties of PP/LDPE composite film: effect of montmorillonite and poly (lactic acid)
Authors:   , Mooninta Supornpat 
Published: September 2023

In this research, the properties of polypropylene (PP) were improved for single layer film application by incorporating low density polyethylene (LDPE), poly(lactic acid) (PLA) and montmorillonite (MMT). The polymer composites was prepared by melt blending using a twin screw extruder and the film was produced by cast film extruder. The addition of LDPE in PP improved the elongation at break and tear strength. The PP/LDPE blend at the ratio of 80:20 (w/w) was selected for blending with PLA and MMT and the PP/LDPE/PLA/MMT composite showed the increased tensile modulus while the elongation at break decreased with increasing PLA and MMT contents. The morphological structure of nanocomposite was examined by using X-ray diffraction (XRD) analysis, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). For the barrier properties, the incorporation of PLA and MMT in PP/LDPE blend tended to decrease the oxygen permeability but increase the water vapor permeability. The 80/20/25/3 PP/LDPE/PLA/MMT composite film exhibited the good balance of mechanical and gas permeability properties and had a potential to be used as a food packaging. Moreover, the PP/LDPE/PLA/MMT composite as a new effective film could yield the decreased tomato loss weight at a low temperature storage (14-20 ºC) rather than PP film.
Protective effects of eriodictyol on hydrogen peroxide-induced cell death in SH-SY5Y cells
Authors:   , Thongin Saowarose 
Published: September 2023

Oxidative stress and mitochondrial dysfunction are the major causes of neuronal death in neurodegenerative disorders. Eriodictyol is a flavonoid extracted from the whole plant of Dendrobium ellipsophyllum (thai name: Ueang Thong). The antioxidant and anti-inflammatory effects of eriodictyol have been widely reported. The present study aimed to investigate the protective effect and mechanisms of eriodictyol on hydrogen peroxide (H2O2)-induced cell death in human neuroblastoma SH-SY5Y cells. The protective effect of eriodictyol on cytotoxicity induced by H2O2 was evaluated using resazurin assay. The effects on intracellular reactive oxygen species (ROS) and mitochondria membrane potential were evaluated by 2´-7’ dichlorodihydrofluorescein diacetate (DCFH-DA) assay and tetramethylrhodamine ethyl ester (TMRE) assay, respectively. The effects of eriodictyol on mitochondria-mediated apoptosis cascade were determined using western blot analysis. The results showed that pretreatment with eriodictyol (0.1, 1 and 10 µM) increased cell viability (p < 0.001), reduced intracellular ROS (p < 0.05) and increased TMRE fluorescent intensity (p < 0.05) compared to H2O2-treated alone. H2O2 induced mitochondria-mediated apoptotic signalling by decreasing Bcl-2/BAX ratio, increasing the expressions of cytochrome c, caspase-3 and cleaved caspase-3 while eriodictyol pretreatment reduced these events. In conclusion, eriodictyol can protect against H2O2-induced cytotoxicity in SH-SY5Y cells via an antioxidant effect, and consequently inhibit mitochondria-mediated apoptosis cascade.
Anticancer effects of mansonone G derivative on human colorectal cancer cells
Authors:   , Chanvijit Savinee 
Published: September 2023

Mansonone G (MG), 1,2-naphthoquinone, isolated from the heartwood of Mansonia gagei Drumm (Chan-Cha-Mod), exhibited several pharmacological effects including anti-bacterial, anti-estrogenic and anti-adipogenic effects. However, anticancer activity of MG and its derivatives on colorectal cancer (CRC) has never been investigated. Therefore, the objective of this study was to investigate the cytotoxic effect of MG and its derivatives and to determine the mechanism(s) underlying cytotoxicity of the most potent MG derivative on two CRC cell lines, HCT-116 cells carrying p53 wild-type and HT-29 cells carrying p53 mutant. In the present study, MG and its derivatives could inhibit viability of HCT-116 and HT-29 cells in a concentration-dependent manner. Of all MG and its derivatives, G07 was the most potent cytotoxic agent toward the cancer cells and less toxic to normal cells, PCS201-010 and CRL-1790. Mechanistic studies revealed that G07 could induce apoptosis through ROS generation in both HCT-116 and HT-29 cells and this effect was abolished by N-acetyl cysteine (NAC). Western blot analysis revealed that G07 downregulated the expression of Bcl-2 and Bcl-xl proteins in both cells and upregulated the expression of Bak protein in HT-29 cells. Moreover, G07 downregulated AKT signaling pathway and modulated ERK1/2 signaling pathway by inhibiting ERK1/2 phosphorylation in HCT-116 cells and activating ERK1/2 phosphorylation in HT-29 cells. Taken together, the present study demonstrated that G07 exerted a potent anticancer effect toward human colorectal cancer cells which was associated with induction of apoptosis, generation of ROS and modulation of ERK1/2 and AKT signaling pathways, suggesting that G07 is a promising compound for further development as an anticancer agent in CRC treatment.
Cross-cultural adaptation and psychometric properties of the Thai version of the Patient-Reported Outcomes Measurement Information System-29 (PROMIS-29)
Authors:   , Rawang Polake 
Published: September 2023

Abstract

Purpose To culturally adapt the Patient-Reported Outcomes Measurement Information System-29 into Thai (T-PROMIS-29) and evaluate the reliability and validity of the culturally adapted questionnaire.

Methods The translation was performed using the Functional Assessment of Chronic Illness Therapy (FACIT) translation guidelines. Internal consistency and test-retest reliability at a 1-week interval for the translated measure were computed. Construct validity was evaluated by computing correlations between the T-PROMIS-29 scores and selected SF-36 and Fear Avoidance Belief Questionnaire (FABQ) scale scores for both convergent and discriminant validity.

Results The study sample comprised of 241 participants with chronic low back pain. Internal consistencies were good to excellent, with Cronbach’s alphas ranging from 0.83 to 0.94. The test-retest reliability of all T-PROMIS-29 domains were moderate to good, with ICCs(2,1) ranging from 0.57 to 0.74. Convergent and discriminant construct validity were satisfactory with moderate correlation coefficients in convergent validity (Spearman correlation coefficients 0.45 to 0.67) and low correlation coefficient in discriminant validity (Spearman correlation coefficients 0.17 to 0.48).

Conclusions The findings support the reliability and validity of the T-PROMIS-29 scale scores. The measure can be used to assess key quality of life domains in individuals from Thailand with chronic low back pain.
Color Stability, Water Sorption And Water Solubility Of Various Artificial Acrylic Resin Teeth
Authors:   , Foungfu Piyaporn 
Published: September 2023

Color stability, water sorption and water solubility of the acrylic resin artificial teeth are the success keys to the appearance and mechanical properties of the denture teeth. The objective of the present study was to compare the color stability, water sorption and water solubility of 3 commercial artificial teeth: linear PMMA (NEW), resin composite (CPX) and cross-linked PMMA (ANT) and 1 color-liked heat-polymerized acrylic resin (MAJ). 80 colume-shaped specimens (9 mm/ 2 mm thickness) were fabricated for color stability test using a spectrophotometer after immersing in water or coffee solution at 1, 7, 28, 56 and 84 days. 40 colume-shape specimens ( 9 mm/ 0.5 mm thickness) were fabricated for the water sorption and water solubility tests using the weight measurement method at 1, 8, 36 and 92 days. The repeated 2-way ANOVA was calculated at the confidential level of 95. The results demonstrated the different color change depending on the type of denture teeth (MAJ>NEW>CPX>ANT) from 28 to 84 days, while the color change showed no significant difference at 1 day storage. The water sorption results demonstrated the greatest MAJ, followed by NEW, CPX and ANT respectively at 36 and 92 days, while the water solubility showed the greatest MAJ, followed by CPX, NEW and ANT respectively. This study confirmed the effect of the composition of acrylic resin denture teeth on the color stability, water sorption and water solubility. Moreover, the effect of the fabrication method as well as the type of storage solution was reported.