bluuebunny/crossref_metadata_2025_split huggingface dataset:
Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes)
Authors: Bao Dan-Feng , Hyde Kevin D. , Maharachchikumbura Sajeewa S. N. , Perera Rekhani H. , Thiyagaraja Vinodhini , HONGSANAN Sinang , Wanasinghe Dhanushka N. , Shen Hong-Wei , Tian Xingguo , Yang Li-Quan , Nalumpang Sarunya , Luo Zong-Long 
Published: March 2023

Abstract
Hypocreomycetidae is a highly diverse group with species from various habitats. This subclass has been reported as pathogenic, endophytic, parasitic, saprobic, fungicolous, lichenicolous, algicolous, coprophilous and insect fungi from aquatic and terrestrial habitats. In this study, we focused on freshwater fungi of Hypocreomycetidae which resulted 41 fresh collections from China and Thailand. Based on morphological and phylogenetic analyses, we identified 26 species that belong to two orders (Hypocreales and Microascales) and six families (Bionectriaceae, Halosphaeriaceae, Microascaceae, Nectriaceae, Sarocladiaceae and Stachybotryaceae). Ten new species and 13 new habitats and geographic records are introduced. Mariannaea superimposita, Stachybotrys chartarum and S. chlorohalonatus are recollected from freshwater habitats in China. Based on phylogenetic analysis of combined LSU, ITS, SSU, rpb2 and tef1-α sequences data, Emericellopsis is transferred to Hypocreales genera incertae sedis; Pseudoacremonium is transferred to Bionectriaceae; Sedecimiella is placed in Nectriaceae; Nautosphaeria and Tubakiella are excluded from Halosphaeriaceae and placed in Microascales genera incertae sedis; and Faurelina is excluded from Hypocreomycetidae. Varicosporella is placed under Atractium as a synonym of Atractium instead of Varicosporella. In addition, phylogenetic analysis and divergence time estimates showed that Ascocodina, Campylospora, Cornuvesica and Xenodactylariaceae form distinct lineages in Hypocreomycetidae and they evolved in the family/order time frame. Hence, a new order (Xenodactylariales) and three new families (Ascocodinaceae, Campylosporaceae, and Cornuvesicaceae) are introduced based on phylogenetic analysis, divergence time estimations and morphological characters. Ancestral character state analysis is performed for different habitats of Hypocreomycetidae including freshwater, marine and terrestrial taxa. The result indicates that marine and freshwater fungi evolved independently from terrestrial ancestors. The results further support those early diverging clades of this subclass, mostly comprising terrestrial taxa and freshwater and marine taxa have been secondarily derived, while the crown clade (Nectriaceae) is represented in all three habitats. The evolution of various morphological adaptations towards their habitual changes are also been discussed.
The impact of personality traits on the positive mental health of Canadian adults during the COVID-19 pandemic: A Replication and Extension Study
Authors: Shokrkon Anahita , Nicoladis Elena 
Published: March 2023

Abstract
The COVID-19 epidemic was first reported in 2019 and rapidly spread across the globe. Many studies have shown that the COVID-19 pandemic adversely affected mental health. Individual differences such as personality could influence people’s responses to the pandemic. For example, the results of a previous study by Shokrkon and Nicoladis (2021) showed that the personality trait of Extroversion positively and Neuroticism negatively contributed to the mental health of Canadians. The goal of our study was to replicate this study using the same tasks and a similar population and extend it by including all 5 personality traits in our analysis and also controlling for the variable of Response to COVID-19 Stress (in addition to demographic variables). Our results were similar to Shokrkon and Nicoladis (2021) and we also found that Extroversion positively and Neuroticism negatively are associated to the mental health of Canadians. We also found that Agreeableness, Openness to Experience, and Conscientiousness are positively and significantly related to the mental health of Canadians. Our results could provide a guide for the screening of people more at risk for mental health issues based on personality traits.
Block-based teaching method based on cybernetics: A trial with 115 Chinese undergraduate medical students
Authors: Liu Zhi-Ping , Liu Si-Han , Dai Xin , Chen Jie , Guo Qing-Feng , Zhang Da-Xin 
Published: March 2023

Abstract
Background: The number of medical undergraduate students in Chinese college classes ranges from dozens to over 100. As a result, the interaction between teacher and students is difficult to conduct. Student attendance and participation need to be improved.
Methods: Two classes of fourth-year undergraduate medical students, with each class comprising 115 students, participated in the study. One class was the trial group taught by the block-based teaching method based on cybernetics. This study was conducted with three of the courses in the Introduction to Oncology. The course of the trial group included several blocks. Each block had a test paper, which was answered immediately by students in class using the Internet. The teacher received feedback from the students when the correct rate of response was less than 90%. The teacher adjusted the teaching in the following blocks according to feedback information. The other class was the control group being taught with the traditional lecture-based teaching method.
Results: The average number of attendees in the trial group was 104/115 (90.43%) and that in the control group was 83/115 (72.17%) (p=0.0003). The teacher adjusted teaching three times in the radiotherapy course due to the difficulty of knowledge. Feedback and adjustment of the upper body of chemotherapy was once and the lower body of chemotherapy was once due to the students’ attitudes to the class. The average total score of the trial group was 86.06 ± 7.46 and that of the control group was 80.38 ± 6.97 (p=0.041). Questionnaire I showed that the trial group students’ attendance and participation was better than the control group. Questionnaire II showed that the block-based teaching method based on cybernetics was approved by the students.
Conclusions: The block-based teaching method based on cybernetics had good effects in medical classes with large number of Chinese undergraduates.
Staphylococcus aureus contamination of animal-derived foods in Nigeria: A systematic review, 2002 - 2022
Authors: Odetokun Ismail Ayoade , Adetona Mutiat Adenike , Ade-Yusuf Rodhiat Oyinlola , Adewoye Adeola Oluwafunmibi , Ahmed Aliyu Nuhu , Ghali-Mohammed Ibrahim , Al-Mustapha Ahmed Ibrahim , Fetsch Alexandra 
Published: March 2023

Abstract
Background: Staphylococcus aureus (S. aureus) is a bacterium of public health importance. The zoonotic spread of this pathogen through animal-derived foods has been reported. This systematic literature review investigates the prevalence, distribution, antimicrobial resistance (AMR) profiles, and molecular characteristics of S. aureus in the food chain in Nigeria.
Methods: A systematic search of online databases (Pub Med, Google Scholar, and Web of Science) for published articles from January 2002 to January 2022 was performed using the Prisma guideline.
Results: Fifty articles were included from an initial 511 extracted documents. These papers included research carried out in 22 states across Nigeria. S. aureus detection in most studies was above the satisfactory level for foods (≥ 104CFU/g). The prevalence of S. aureus ranged from 1.3% in raw cow meat to 72.5% in fresh poultry meat. Most S. aureus isolates demonstrated multiple drug resistance patterns, especially being resistant to beta-lactams. There is a lack of information on the molecular typing of the S. aureus isolates. The different spa types of S. aureus isolated were t091, t314, t1476, and t4690, categorized into Multi-Locus-Sequence Types ST8, ST121, ST152, and ST789. Virulence genes detected include pvl, sea, see, spa, coa, edin, tsst, and hly. Certain AMR-encoding genes such as mecA, blaZ, fos, tet, and dfsr were detected. Factors contributing to the presence of S. aureus were reported as poor processing, poor sanitary conditions of the food processing units, inadequate storage units, and poor handling.
Conclusion: We showed that S. aureus is a major food contaminant in Nigeria despite the lack of information on the molecular typing of strains from animal-derived food sources. There is a need to control S. aureus by targeting specific entry points based on the findings on risk factors and drivers of food contamination.
Inhibitions from syngas impurities: impact of common tar components on a consortium adapted for syngas biomethanation
Authors: Figueras Julie , Benbelkacem Hassen , Dumas Claire , Buffiere Pierre 
Published: March 2023

Abstract
In a circular economy approach, syngas biomethanation is a promising technology for waste to energy conversion. However, syngas can contain impurities, notably tar, that can limit the processes upgrading syngas downstream gasification. The effect of tar on syngas biomethanation is unknown. Therefore, for the first time, common tar components were tested on a consortium adapted for syngas biomethanation to assess the resistance of the microorganisms. Four light tar components (benzene, toluene, styrene and phenol) commonly found in syngas were selected and tested at different concentrations in batch tests. Adding pollutant up to inhibitory concentrations affected both the lag phase and the rates of the bioreactions. Hydrogenotrophic methanogens were found to be more sensitive than carboxydotrophs. Amongst the four tested pollutants, phenol appears to be the most problematic, due not only to its high inhibitory effect but also to its high solubility, allowing phenol in the syngas to reach high inhibitory concentrations. This study paves the way for further research on the resistance of syngas biomethanation to impurities contained in the syngas.
Elucidation of interfacial microstructure inhomogeneity and crack defects of laser fusion brazing AA5083/TA31 dissimilar butt joints at different groove angles
Authors: Li Yang , Zhang Shuaifeng , Tang Xinhua , Zhang Yunhao , Zhang Maofu , Lv Yifan 
Published: March 2023

Abstract
Al/Ti dissimilar alloy plates with thickness of 6 mm were butt joined using the direct laser fusion brazing method. A systematic comparison of laser fusion brazing of four groups of AA5083 to TA31 plates with different groove angles ranging from 60° to 90° was performed by laser beam offsetting to the aluminum alloy side. The related weld appearance, microstructures in weld and at joint interface, defects formation mechanism and fracture resistance were analyzed and compared in detail. Preferable joint appearance, characterized by wetting the top and bottom sides of the titanium alloy with molten aluminum alloy, can be obtained except for the joints with 60° groove angles. The uniformity of intermetallic compound (IMC) morphology and thickness distribution at the interface was improved as the groove angle decreases, accompanied by a decrease in the diffusion distance of main elements at the same location. Severe interfacial crack and intergranular hot crack defects were formed in the joints with 90° and 60° groove angles, respectively. The interfacial cracks are induced by the difference in hardness and elastic modulus between TiAl3 phases and titanium base metal or aluminum weld. The hot crack defects are mainly caused by microstructure mismatch including grain size and texture orientation mismatch between the fine grain zone near the interface and the coarse grain zone inside the aluminum alloy weld. The crack-free joints with groove angles of 70° show the highest tensile strength of 268.76 MPa due to the relatively uniformly distributed and fine interfacial IMCs. This study reveals the possibility to realize efficient joining of medium-thick titanium alloy and aluminum alloy plates by using laser fusion brazing method without other auxiliary equipment.
Autophagy-enhancing ATG16L1 polymorphism improves clinical outcomes and promotes superior T-cell immunity in chronic HIV-1 infection
Authors: Schreurs Renée , Koulis Athanasios , Booiman Thijs , Boeser-Nunnink Brigitte , Cloherty Alexandra , Rader Anusca , Patel Kharishma , Kootstra Neeltje , Ribeiro Carla 
Published: March 2023

Abstract
Chronic HIV-1 infection is characterized by T-cell dysregulation that is only partly restored by antiretroviral therapy. Here, we demonstrate a protective role for autophagy in HIV-1 disease pathogenesis. Targeted analysis of genetic variation in core autophagy gene ATG16L1 revealed a previously unidentified polymorphism, which correlated functionally with enhanced autophagy and clinically with improved survival of untreated HIV-1-infected individuals. Intrinsically-enhanced autophagy in homozygous minor T-cells resulted in superior antiviral immunity, evidenced by increased proliferation, revamped immune responsiveness, and suppressed exhaustion/immunosenescence features. In-depth flow-cytometric and transcriptional T-helper-profiling revealed signatures unique to minor genotyped individuals with enriched regulation of pro-inflammatory networks and skewing towards immunoregulatory T-cell-phenotype. Treatment with autophagy-enhancing pharmaceuticals reprogrammed T-cells to exhibit the protective traits observed in homozygous minor donors. These data underscore the in vivo relevance of autophagy for longer-lasting T-cell-mediated HIV-1 control, with implications towards development of host-directed therapeutics targeting autophagy to restore immune function in chronic HIV-1 infection.
The Property Rights Index (PRIF) can be used worldwide to compare different forest governance systems
Authors: Rimoli Richard , Nichiforel Liviu , Acharya Aditya , Nollet Alexandre , Snoussi Bilal , Ambroise Lison , Cordonnier Louis , Mares Sandra Galván , Zúñiga José Jonathan Aguirre , Bontemps Jean-Daniel , Bouriaud Laura 
Published: March 2023

Abstract
The bundle of forest landowners’ rights largely varies from one jurisdiction to another. On a global scale, the diversity of forest regime and property rights systems is such that finding similarities for governance analysis purposes is a challenging task. This paper built on the Property Rights Index for Forestry (PRIF). We show that PRIF is a reliable index for various governance arrangements, from forests privately owned in Europe, in the Amazon area (Brazil), in Misiones province (Argentina) and in Quebec (Canada) to community-managed Nepalese forests. The PRIF enables us to compare accurately and efficiently the forests regime incident to different types of forest ownership on three continents with an integrative and standardised approach. Despite having a few limitations, the PRIF is a promising indicator to assess governance of the natural resources in various socioeconomic and legal contexts.
Perception of medical students on their education environment: A quantitative study
Authors: Muganzi David Jolly , Kintu Timothy Mwanje , Nakandi Racheal Mukisaa , Atulinda Linda , Atim Immaculet Kaducu , Mwaka Francis , Kiiza Blair , Ngonzi Joseph 
Published: March 2023

Abstract

Background
Health care workforce is one of the six core components of a well-functioning and sufficient health system. The WHO highlights improving education and training of health workforce as one of the areas of focus for improving health. Medical education is a pillar of the health care workforce. Educational environment, the physical circumstances or conditions under which learning takes place is an important factor that determines the effectiveness of medical education. It’s academic and clinical effects are significant determinants of medical students’ attitudes, knowledge, skills, progression and behaviours. To ensure strong health systems, there is need for improvement in medical education. This has to be contextual and informed by locally relevant data. This study aimed at assessing Ugandan medical students’ perception of their learning environment.
Methods
This was a quantitative cross-sectional study among medical students across five medical schools in Uganda between December 2021 and February 2022. Participants filled an online questionnaire with a demographics section and the Dundee Ready Education Environment Measure (DREEM) tool. The data was analyzed using R Studio version 2021.09.0 + 351. The average score for each of the DREEM sub-scales and total DREEM score were calculated in addition to their relationship with demographic characteristics
Results
The average total DREEM score of the 335 participants was 112.27/200 (56.12%). We found that 69% (231/335) of the students had a positive perception while 31% had a negative perception of their learning environment. The sub-scale mean scores were as follows; Perception of Atmosphere – 25.14/48(52%), Social Self-perception − 14.94/28 (53%), Perception of Teachers − 24.86/44 (56%), Perception of Learning − 28.8/48 (59%) and Academic Self-perceptions – 19.04/32 (60%). “There is a good support system for students who get stressed” was the item with the lowest mean score (1.41/5)
Conclusions
The perception of medical students on their learning environment is just above average. This could negatively impact the quality of the health workforce. To ensure training of a robust health workforce, there is need for improvement in learning environment especially the atmosphere and social support.
Effect of silica nanoparticles and modified silica nanoparticles on the mechanical and swelling properties of EPDM/SBR blend nanocomposites
Authors: Ragupathy K. , Prabaharan G. , Pragadish N. , Vishvanathperumal S. 
Published: March 2023

Abstract
The influences of nanosilica and modified nanosilica on cure, compression set, mechanical and swelling characteristics are studied in blends of ethylene-propylene-diene monomer (EPDM)/styrene-butadiene rubber (SBR). In EPDM/SBR composites, the nanosilica made from rice husk ash (RHA) was utilised as a reinforcing nanofiller. The interaction between the nanosilica and the EPDM/SBR matrix at the interface is crucial for the production of rubber composites. This study uses bis[3-(triethoxysilyl)propyl] tetrasulfide (TESPT) and polyoxyethylene (20) sorbitan monolaurate (TWEEN-20) as a silica modifier, with the ratio of TESPT to TWEEN-20 maintained at 2:1. The cure and mechanical characteristics, compression set, abrasion and swelling resistance increased with the incorporation of nanosilica and modified nanosilica. By interacting at the interface between silica nanoparticles and the EPDM/SBR rubber matrix, TESPT with the TWEEN-20 modifier exhibits a considerable effect on cure characteristics, mechanical qualities, and swelling resistance.
To Evaluate Impact on Detection Rate of Streptococcus Agalactiae in the Third Trimester of Pregnancy
Authors: Jiang Yaping , Zhou Jia , Li Zhiyan , Sun Liying 
Published: March 2023

Abstract
Background: The streptococcus agalactiae infectious leading to early neonatal morbidity and mortality,the streptococcus agalactiae screen became an important examination for pregnant women. The Centers for Disease Control and Prevention (CDC) recommends whole detection protocols of isolation and culture on streptococcus agalactiae.However, The essential factors including incubation time of agar plates, enrichment time of broth, and transport media storage conditions be ignored. This study was designed to understand above easy ignored point and to provide scientific proof for optimization detection method, revision of health standards and prevention and control of disease.
Methods: The transport medium without and with enrichment were directly inoculated onto Sheep blood agar plate for 24~48 hours. The positive detection rate of streptococcus agalactiae on different incubation time, with and without enrichment were compared,respectively. The transport medium with enrichment were inoculated for 24~48 hours.The positive detection rate of streptococcus agalactiae on different enrichment time were compared. The transport medium were respectively stored at 2℃~8℃ and 20℃~25℃. Interval 24 hours within 5 days, The recovery rate of streptococcus agalactiae on transport medium different storage temptature were compared. The growth of streptococcus agalactiae colonies were examined for pale pink to red, round and pearly colonies. The isolates colonies were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry to confirm negative or positive.
Results: The overall results shown that the detection rate were significantly higher at 48 hours with directly culture methods (P＜0.01)or after enrichment culture methods (P＜0.05). The detection rate have no difference after enrichment for 24 hours or 48 hours (P＞0.05). The recovery rate of streptococcus agalactiae transport medium (stored at 2℃~8℃) were 100% within 4 days and 83.33% at 5th day. However, the recovery rate of streptococcus agalactiae transport medium (stored at 20℃~25℃) were 100% within 2 days,97.22% at 3th day, 52.78% at 4th day and 33.33% at 5th day, respectively.
Conclusions: In conclusion, the results suggested that the enrichment culture time was 24 hours, the transport media stable best time is 2 days stored at 20℃~25℃ or 4 days stored at 2℃~8℃, and the best incubation time is 48 hours for streptococcus agalactiae clinical testing in pregnant women.
Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments
Authors: Torii Takeru , Sugimoto Wataru , Itoh Katsuhiko , Kinoshita Natsuki , Gessho Masaya , Goto Toshiyuki , Uehara Ikuno , Nakajima Wataru , Budirahardja Yemima , Miyoshi Daisuke , Nishikata Takahito , Tanaka Nobuyuki , Hirata Hiroaki , Kawauchi Keiko 
Published: March 2023

Abstract
Tumor suppressor p53 plays a central role in the DNA damage response. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, it remains unclear whether p53 affects the structure of nuclear actin filaments. Here, we show that actin filament formation in the nucleus induced by the DNA-damaging agent doxorubicin (DOXO) treatment is suppressed by p53 via caspase-1 expression. In DOXO-treated cells, the nuclear actin filament formation was promoted by p53 depletion. Whilst the DOXO treatment caused an increase in caspase-1 expression, which was largely attenuated by p53 depletion, overexpression of caspase-1 reduced DOXO-induced formation of nuclear actin filaments in p53-depleted cells. By contrast, caspase-1 inhibition, together with DOXO treatment, induced nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that p53-mediated expression of caspase-1 suppresses nuclear actin filament formation. In addition, we found that expression of nLifeact-GFP, which contains the filamentous actin (F-actin)-binding peptide and nuclear localization signal (NLS), promoted the bundling of nuclear actin filaments and strongly altered the chromatin structure in DOXO-treated, p53-depleted cells. Given that the loss of p53 is associated with cancer progression, the results of this study raised the possibility that artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxicity effect of DNA-damaging agents in aggressive cancer cells.