bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análise integrada da alteração do número de cópias de DNA e expressão de miRNAs em tumores mamários triplo-negativos
Authors: Ribeiro Enilze Maria de Souza Fonseca 
Published: January 2023

Os carcinomas mamários triplo negativos (TNBC) apresentam em sua maioria uma evolução clínica agressiva, com prognóstico desfavorável. Os microRNAs são uma classe de RNAs não-codificantes endógenos que regulam genes responsáveis por diversos processos biológicos celulares, entre eles a proliferação e sobrevivência celular. Por regularem mais de um gene, uma alteração na expressão de um único miRNA pode resultar na desregulação de diversos genes, apresentando potencial como biomarcadores moleculares. Alguns miRNAs apresentam expressão diferencial em TNBC comparados com tumores não-triplo negativos (NTNBC), apresentando poder discriminatório na classificação de tumores mamários. Com o objetivo de selecionar miRNAs diferencialmente expressos de uma forma mais estrita, integramos os dados de alterações de número de cópias de DNA - CNAs (gerados por aCGH genômico) com os dados de expressão de miRNAs (gerados pela plataforma Nanostring). Cinquenta e sete pacientes foram estudadas (29 classificadas como TNBC e 28 NTNBC) a partir de amostras parafinizadas. A análise de expressão resultou em 209 miRNAs diferencialmente expressos em TNBC quando comparados com NTNBC (Teste t, p<0,05). O mapeamento dos miRNAs diferencialmente expressos nas suas respectivas regiões cromossômicas resultou em 27 miRNAs presentes em regiões mais comumente afetadas por CNAs. Entre estes, 12 apresentaram concordância entre as duas abordagens, isto é, ganho de número de cópias e aumento de expressão ou diminuição do número de cópias e da expressão. A integração dos dados de CNAs e miRNAs também foi realizada. Um total de 5.859 genes alvos dos 12 miRNAs (determinados por três ferramentas de predição) foram identificados e comparados com os genes localizados nas regiões com CNAs (n=5.296 genes). A integração desses dados resultou em 1.312 genes em comum, reduzindo em 77,6% a lista de genes alvos. A fim de determinar possíveis vias de sinalização afetadas pelos miRNAs selecionados, o software Diana miRPath v3.0 foi utilizado. As principais vias de sinalização afetadas incluem Via de Sinalização Hippo (hsa04390), Biossíntese de Glicosaminoglicanos (hsa00534) e Via de Sinalização do TGF-beta (hsa04350). Finalmente, a análise ROC determinou um bom poder discriminatório individual e do conjunto dos miRNAs em discriminar os grupos TNBC e NTNBC.
Alterações cromossômicas nas síndromes de Prader-Willi e Angelman
Authors: Nascimento Flavia Caroline , Rezende Lúciana Montes , Santos Ana Paula dos , Sgardioli Ilária Cristina , Viguetti Campos Nilma Lúcia , Simioni Milena , Steiner Carlos Eduardo , Bertuzzo Carmen Sílvia , Gil da Silva Lopes Vera Lúcia , Vieira Társis Antônio Paiva 
Published: January 2023

As síndromes de Prader-Willi (SPW) e Angelman (SA) possuem características clínicas distintas e suas prevalências são de :5.000 a :25.000 nascimentos. A etiologia dessas síndromes está relacionada com alterações na região q a q3 do cromossomo 5, que contém um grupo de genes imprintados. O objetivo deste trabalho foi realizar um estudo retrospectivo de pacientes com suspeita clínica de SPW e SA, e realizar análises citogenéticas e moleculares complementares. Foram revisados os resultados de cariótipo e teste de metilação. As técnicas de Hibridação in situ fluorescente (FISH) com a sonda MD IGFR(5q26)/SNRPN/UBE3A(5q) – Podeidon-Kreatech Diagnostics; e hibridação genômica em array (aGH) utilizando a plataforma CytoScan 750 K array (Affymetrix® Microarray Solutions, Santa Clara, CA) foram empregadas em casos pertinentes. Dos 30 pacientes analisados, o teste de metilação confirmou nove de 23 casos com suspeita de SPW e um de sete casos com suspeita de SA. Todos os pacientes com suspeita clínica de SPW e teste de metilação normal, também possuíam cariótipo normal. Entre os pacientes que tiveram a SPW confirmada através do teste de metilação, sete possuíam resultado de cariótipo normal e dois tinham cariótipo alterado. Em um caso foi identificada a deleção 5q2q2, confirmada por FISH, e, no outro caso, foi encontrada uma translocação Robertsoniana envolvendo os cromossomos 4 e 5. O resultado de FISH foi normal, enquanto a técnica de aGH mostrou uma dissomia uniparental (DUP) segmentar materna em 5q.2-q4. Entre os pacientes com suspeita clínica de SA com metilação normal, cinco tiveram resultado de cariótipo normal e uma apresentou uma inserção entre os cromossomos 9 e 5. O resultado de aGH desta paciente também foi normal. O paciente com a síndrome confirmada pelo teste de metilação possui resultado de cariótipo alterado e a análise do cariótipo dos familiares permitiu constatar que se trata de um rearranjo cromossômico herdado da família materna do paciente. Através dos resultados obtidos conclui-se que o teste de metilação é eficiente para a confirmação do diagnóstico de SPW e SA, porém, os casos relatados neste trabalho, associados a rearranjos cromossômicos, mostram que a análise citogenética contribui para o aconselhamento genético adequado das famílias. Apoio financeiro: FAEPEX-UNICAMP.
Dengue Infected Patients in Local Population of Lahore
Authors: Akhtar Khunsa 
Published: January 2023

Over the past 20 years, dengue fever's demographic characteristics have significantly altered in Pakistan. Different facets of particular epidemics have been covered in small-scale research from around the nation during this time. However, there is a dearth of information on the country's general trend of dengue virus infection. Objectives: To examine liver function test of dengue infected patients, age and gender wise distribution of dengue virus infected patients. Methods: During August-November 2011, patients with clinical presentation of DF, who came to Emergency department of Mayo Hospital Lahore, were included in this study. Total 64 patients were included in this study and LFTs were performed photometrically to see the effect of dengue virus infection on liver of affected patients during early days of infection from 2-7 days. Results: Out of 64 patients, 47 were males and 17 were females. Dengue fever was more common among the age group of 16-25 years and less common among children and older age group. All the patients had low platelet level with 29 patients had complaint of bleeding. Serum raised level of ALT and ALP was observed in some patients. Conclusions: Presently widespread throughout the year with a high frequency in the post-monsoon season, dengue fever is currently endemic in Pakistan. The median patient age for dengue has dropped, and younger patients, particularly men, may be more vulnerable. Total and differential leukocyte counts can assist identify individuals who are at risk for bleeding, and LFTs can show whether dengue virus infection has an impact on the liver.
Treatment of older patients with multiple myeloma
Authors:   , Stege Claudia Antoinette Maria 
Published: January 2023

General discussion
Over the last decades, the prognosis of non-transplant eligible (NTE) patients with newly diagnosed multiple myeloma (NDMM) has improved substantially.1-3 However, this benefit is less in older patients, both when treated within clinical studies as in those patients receiving standard of care.2,4-6 There are several explanations for the inferior survival of older patients with MM. Firstly, older patients more often receive doublet therapies, instead of the generally more potent triplets. Secondly, older patients experience more pronounced treatment related toxicity hampering optimal treatment, leading to relapse or may even lead to death.7 Thirdly, older patients are less likely to receive a subsequent line of treatment, which might be due to comorbidities, persistent toxicity related to former treatment, or the patient’s wish for palliation.8 Finally, older patients may receive no treatment at all because physicians feel reluctant to treat them of fear for treatment related complications.2,9,10 This in turn may lead to under-treatment of a relatively fit subgroup of patients, causing inferior outcome.11,12
In order to improve the outcome of older patients with NDMM, it is needed:
I.	to identify fit versus non-fit patients, in order to personalize treatment;
II.	to perform clinical trials to investigate the efficacy of treatment regimens in older patients according to their frailty level;
III.	to obtain meticulous data on side effects and quality of life (QoL), since older non-fit patients might value side effects and the effect of treatment on QoL as more important factors that impact the choice for treatment than length of life only.13,14
This thesis aimed to fill part of these knowledge gaps in the treatment of NTE patients with NDMM.
In PART I we validated the current gold standard of frailty assessment in MM, the IMWG frailty index (IMWG-FI), as well as the simplified frailty index that is more commonly used in clinical trials. We refined the IMWG-FI in order to improve the prognostic value for outcome.
In PART II we investigated the value of novel treatment options that were hypothesized to be suitable for non-fit older patients with NDMM.
in PART III we investigated the impact of treatment on health-related quality of life (HRQoL).
TINGKAT PENGETAHUAN IBU TENTANG PENANGANAN DEMAM PADA ANAK DI APOTEK SEHAT SUKOHARJO
Authors: Puspitosari Adik , Hartono Hartono 
Published: January 2023

ABSTRAK
Demam adalah tanda tubuh sedang melawan infeksi atau bakteri yang membuatnya sakit. Penanganan demam pada anak tergantung pada peran orang tua terutama ibu, sehingga diperlukan pengetahuan dalam menangani demam. Tingkat pengetahuan ibu sangat bervariasi sehingga terdapat perbedaan penanganan demam pada anak. Penelitian ini bertujuan untuk mengetahui gambaran tingkat pengetahuan ibu tentang penanganan demam pada anak di Apotek Sehat Sukoharjo. Jenis penelitian ini deskriptif, dengan metode yang digunakan adalah purposive sampling. Pengumpulan data dilakukan dengan menggunakan kuesioner dengan sampel 140 orang responden. Hasil penelitian menunjukkan responden mempunyai pengetahuan baik tentang definisi demam, mekanisme demam, penyebab demam, pengukuran suhu, obat demam, dosis obat, dan penyimpanan obat. Hampir semua responden (91%) belum memahami bahwa tidak semua demam merupakan keadaan berbahaya sehingga harus segera diturunkan dan akan terus meningkat jika tidak segera diturunkan dan terdapat 66% ibu masih salah dalam penggunaan kompres air dingin untuk menurunkan demam anak. Berdasarkan keseluruhan penelitian maka dapat disimpulkan bahwa gambaran tingkat pengetahuan ibu tentang penanganan demam pada anak di Apotek Sehat Sukoharjo dinyatakan baik sebesar 51,4% (72 orang) dan 45,7% (64 orang) memiliki pengetahuan yang cukup tentang penanganan damam pada anak, sedangkan pengetahuan yang kurang sebesar 2,9% (4 orang)

Kata Kunci: anak, demam, pengetahuan ibu.
HUBUNGAN PENGETAHUAN DAN PERSEPSI MASYARAKAT TERHADAP PENERIMAAN VAKSIN COVID- 19 DI DESA CISANTANA TAHUN 2021
Authors: Lisnawati Lilis , Azizah Nur 
Published: January 2023

Persepsi masyarakat terhadap vaksin Covid-19 merupakan suatu tanggapan atau penerimaan dari informasi yang diperoleh mengenai keyakinan terhadap kehalalan vaksin, kesediaan divaksinasi dan kapasitas tenaga kesehatan yang memberikan vaksin Covid-19. Hal tersebut dikarenakan banyak sekali isu-isu yang mempengaruhi tentang informasi mengenai vaksin Covid-19 seperti faktor kehalalan dan keamanan dari vaksin Covid-19. Penelitian ini bertujuan untuk mengetahui persepsi masyarakat terhadap vaksin Covid-19 di Desa Cisantana. Penelitian ini merupakan penelitian kuantitatif dengan menggunakan desain cross sectional. Pada penelitian ini berjumlah 97 sampel yang diperoleh dari wawancara langsung serta pengisian kuisioner. Analisis data menggunakan Chi Square.
Berdasarkan hasil analisis maka dapat diketahui terdapat hubungan antara pengetahuan dan persepsi masyarakat dengan penerimaan vaksin Covid-19 di Desa Cisantana dengan p value 0,000 dan 0,002.
Faktor-Faktor Yang Mempengaruhi Kenakalan Remaja
Authors: Afrita Fitri , Yusri Fadhilla 
Published: January 2023

Penelitian ini dilatarbelakangi oleh permasalahan remaja diantaranya terdapat remaja yang terjerumus ke dalam pergaulan bebas dengan teman lawan jenis yang mengakibatkan adanya remaja putri yang hamil di luar nikah. Hal ini juga berdampak pada pendidikannya, dimana ia harus berhenti sekolah akibat pergaulan bebas. Selain itu terdapat remaja yang merokok padahal masih duduk dibangku SMP. Terkadang remaja tersebut suka mengambil barang milik orang lain, terdapat remaja perempuan yang pulang ke rumah sudah larut malam bahkan sampai pukul 2 dini hari. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang mempengaruhi kenakalan remaja di Kelurahan Balai-Balai Kecamatan Padang Panjang Barat Kota Padang Panjang. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian kualitatif yang bersifat deskriptif, yaitu dengan menggambarkan kejadian yang ada di lapangan secara sistematis. Informan atau orang yang bertindak sebagai pemberi informasi dalam penelitian ini adalah Remaja dan orang tua di Kelurahan Balai-Balai Kecamatan Padang Panjang Barat Kota Padang Panjang. Penulis mengumpulkan data dengan teknik observasidan wawancara, kemudian data-data yang diperoleh diolah dengan menggunakan teknik analisa data deskriptif kualitatif dan triangulasi data, yaitu dengan membandingkan data yang diperoleh dari hasil observasi dan wawancara. Berdasarkan hasil penelitian, maka dapat disimpulkan bahwa faktor-faktor yang mempengaruhi kenakalan remaja di Kelurahan Balai-Balai Kecamatan Padang Panjang Barat terdiri dari dua faktor, yaitu faktor internal dan faktor eksternal. Faktor internal diantaranya lemahnya pemahaman remaja terhadap nilai-nilai agama dan tidak ada kesadaran dalam menjalankan kewajiban sebagai umat islam, sehingga para remaja sering meninggalkan kewajiban ibadah shalat, serta lemahnya pertahanan diri remaja sehingga tidak berani menolak ajakan teman yang mengajak melakukan perbuatan yang tidak baik. Sementara faktor eksternal yang mempengaruhi kenakalan remaja yaitu faktor lingkungan rumah tangga dan faktor lingkungan masyarakat sekitar dimana remaja tinggal. Masyarakat yang kurang peduli dan kurang peka menjadi salah satu faktor penyebab kenakalan remaja. Untuk faktor lingkugan sekolah tidak terlalu mempengaruhi kenakalan remaja di Kelurahan Balai-Balai Kecamatan Padang Panjang Barat.
STRATEGY MARKETING DEVELOPMENT OF UMKM (CASE STUDY IN BOYOLALI DISTRICT-CENTRAL JAVA)
Authors: Natalia Arta Malau  , Teguh Pamuji Tri Nur H  
Published: January 2023

The role of the MSME sector is very important for the economic growth of a region. Where this sector reflects the local economy, a region and as a driving force for the regional economy. The existence of the Covid 19 pandemic has caused MSMEs actors to have new challenges and problems, regarding marketing in the form of digitalization. Therefore, a strategy is needed, which is able to answer these problems. This MSME Marketing Development Strategy is to see the types and characteristics of MSMEs, potentials and problems as well as strategies for handling them. The case of this research is MSMEs Boyolali Regency. The results of this study are SWOT analysis, which requires MSME marketing activities based on MSME digitization through increased product marketing through Social Media and E-Commerce.
REASONS FOR APOSTASY OF NEW MUSLIM PEOPLE IN PAKISTANI SOCIETY (RESEARCH AND CRITICAL REVIEW)
Authors: Nisar Dr. Qutab 
Published: January 2023

The areas where Muslims are in majority in Pakistan, no new-Muslim should face any kind of problem and difficulty. They should be treated in such a way that other non-Muslims feel attracted towards Islam. But our society creates such problems for them as they did not face even as a non-Muslim. Perplexed with these problems, they develop thinking about reverting to their previous religion. The basic cause of all these problems is the society based on Hinduism which comprises materialism and caste system. Until and unless this system is rooted out, new Muslims will have to face them. These problems are the biggest hindrance in the way of preaching and proselytizing which do not allow non-Muslims to embrace Islam. Therefore, it is essential to remove these problems and establish our society on Islamic foundations and aware people about Islamic teachings. In addition to this, the government should also announce special incentives for the new-Muslims because the credit for increasing the number of followers of Islam day by day goes to them.
L’angklung américain : spectre d’une rencontre coloniale oubliée
Authors: Bellemare Laurent 
Published: January 2023

En 1900, la compagnie américaine J.C. Deagan Company spécialisée en xylophones a breveté l’invention dudeagan organ chimes, un carillon d’idiophones en métal plaqué de nickel. Composé d’une série d’instruments détachables et correspondant chacun à une note, l’instrument se joue en faisant trembler chaque unité. Le son cristallin qui en résulte est produit par la vibration de l’air dans de petits tubes d’orgue accordés sur une fréquence et ses octaves supérieures. Aujourd’hui, cet instrument a survécu à la dissolution de sa compagnie-mère comme une curiosité de l’histoire de la facture instrumentale américaine. Toutefois, la conception de l’organ chimessuggère une origine antérieure en raison de sa ressemblance frappante avec l’angklungd’Indonésie, un type de percussion en bambou omniprésent à travers l’archipel sud-est asiatique. Jamais explicitement référencée par la compagnie, la clé de cette rencontre se situe vraisemblablement dans la dissémination des cultures d’ailleurs en Amérique à l’époque coloniale. Cet article tente de rapprocher l’invention de cet instrument avec l’événement majeur qu’a été l’Exposition universelle de 1893 à Chicago. Étant donné le grand impact exercé par le village javanais sur l’imaginaire américain, il est peu probable que la création sept ans plus tard de l’organ chimesoit issue d’une simple coïncidence. Paradoxalement, cette invention est rapidement tombée en désuétude auxxe siècle, alors que l’angklunga connu une renaissance en Asie. Appropriation culturelle tombée dans l’oubli, le cas de l’organ chimespermet de repenser le discours historique sur la facture instrumentale et les inventions musicales.
Incidence of Pencil and Pen Eye Injury among Children in Anambra State, South-East, Nigeria: Ten Year Retrospective Study
Authors: Anajekwu Cosmas Chinedu , Eze Gloria Chizoba 
Published: January 2023

Background: Eye injuries constitute a significant portion of most ophthalmologic consultations and ocular emergencies with grave consequences, especially in children, if not managed well early enough. Traumatic eye damage is the leading cause of monocular blindness worldwide. This study aimed to determine the incidence, circumstances and types of ocular injury among children in Anambra state, South-East, Nigeria.
Materials and Methods: A ten- year retrospective study of 211 children aged below 18 years who presented with eye trauma in two mission hospitals in Anambra State, Nigeria. Case file of children with ocular trauma were retrieved from the medical records and those with pencil and pen injury isolated. Required information was extracted from the case files and recorded in a pro-forma. The data was analyzed using Statistical Package for Social Science, Version 20 (SPSS-20).
Results: The incidence of pencil/pen eye injury was 10.9% (23/211). The injuries were commoner in males (M:F=1.6:1) and in those aged five years and below. Stab injuries constituted 56.5% (13) of the injuries while 34.8% (8) were missile injuries. Forty-six percent (6) of the stab injuries were self-inflicted with two-third of this being in children younger than five years. Corneal laceration, followed by anterior uveitis, was the commonest form of eye injury.
Conclusion: There is a high incidence of pencil and pen injury in South-East, Nigerian children. Although visual prognosis is good with early presentation and treatment, adequate preventive measures should be put in place to forestall the occurrence of this potentially blinding condition in children.
Optimization of RNA Extraction Protocol for Rat Skeletal Muscle Samples
Authors: Felipe Stela Mirla da Silva , Pacheco Christina , Martins Jonathan Elias Rodrigues , Freitas Raquel Martins de , Oliveira Paulo Elesson Guimarães de , Mendes Sávio Victor Diógenes , Alves Juliana Osório , Ceccatto Vânia Marilande 
Published: January 2023

Aims: The present study aimed to stablish and characterize an optimized protocol conformation to obtain adequate RNA quality from rodents skeletal muscle samples for sequencing studies.
Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará - UECE. Between 2017-2020.
Methodology: Were used 23 samples from male Wistar rat skeletal muscle, specifically from soleus muscle. Total RNA extraction was performed using the classic TRIzol® method and commercial kit, merging steps from both. Capillary electrophoresis in the Bioanalyzer platform was used for RNA quality evaluation.
Results: (C) Analyzes of adapted protocol RNA concentration, RIN and rate 28S/18S showed satisfactory results. 28S/18S Ribosomal bands appear well defined, without small traces, which indicates RNA with high integrity and without contamination of genomic DNA.
Conclusion: Obtained RNA quality and integrity data satisfied the exigencies for posterior RNA-seq.