bluuebunny/crossref_metadata_2025_split huggingface dataset:
The rescue potentials of OPC-41061, OPC-31260 and OPC-21268 on mutant AVPR2s that cause nephrogenic diabetes insipidus
Authors: SAHIN Dilara , Tuncdemir Beril Erdem , Ozer Emel Saglar , MERGEN Hatice 
Published: July 2023

Abstract
Purpose
Nephrogenic Diabetes insipidus (NDI) is a rare disease which is characterized by polyuria and polydipsia. AVPR2 mutations can cause NDI. Mutants may not function properly since the mutations can affect their folding process and make them be trapped in the Endoplasmic reticulum (ER). Recent studies showed that pharmacological chaperones (PCs) may rescue mutant AVPR2s and they can be functional again. OPC-41061, OPC-31260 and OPC-21268 are these kind of PCs and they can specifically bind to the mutant AVPR2s and rescue them from ER via helping their folding process. In this study, the effects of OPC-41061, OPC-31260 and OPC-21268 on AVPR2 mutants (R68W, ΔR67-G69/G107W, V162A and T273M) were analyzed.
Methods
We performed cell surface and total ELISA, and cAMP accumulation assay for mutant AVPR2s after the treatment with 3 different PCs.
Results
It was observed that OPC-41061 improved cell surface expressions of all mutants except V162A and ΔR67-G69/G107W, and cell surface expressions of R68W and ΔR67-G69 were increased with all three PCs. Then mutants were stimulated with their agonists to measure cAMP accumulation in the cell as a functional analysis. As a result, we found that R68W and ΔR67-G69 (except T273M) were rescued and could be functional after the treatment with OPC-41061 and OPC-31260.
Conclusion
Showing specific rescue potential of these kind of PCs has an importance on the development of new treatment strategies on NDI. Determining the responses of mutant proteins to various chaperones is extremely important for current treatment approaches and identifying the new PCs.
Effectiveness and Safety of Anticoagulants Among Patients with Venous Thromboembolism and Active Cancer Who Also Had Prior Bleed or Prior Renal Disease
Authors: Cohen Alexander , Noxon Virginia , Dhamane Amol D , Shah Shrushti , Hines Dionne M , Alfred Tamuno , Chaves Jose , Luo Xuemei 
Published: July 2023

Abstract

PURPOSE: Patients with active cancer and venous thromboembolism (VTE) have elevated risk of major bleeding (MB) and recurrent VTE (rVTE). The risk is even higher within those with a prior bleeding event or renal disease. There is a need to understand the risk of MB and rVTE of commonly used anticoagulants among these high-risk patients.
METHODS: VTE patients with active cancer and treated with apixaban, warfarin or low molecular weight heparin (LMWH) within 30-days of VTE were identified from 5 claims databases in the United States. Inverse probability of treatment weighting (IPTW) was used to balance patient characteristics. Post-IPTW population was stratified by prior bleed or renal disease status. Cox proportional hazards models were used to evaluate interactions of treatment with prior bleed or renal disease and risk of rVTE and MB,withp-value <0.1 as significant.
RESULTS: 30,586 VTE cancer patients met all study criteria: 35.0% had prior bleed and 29.0% had renal disease. Use of apixaban, warfarin or LMWH was similar regardless of prior bleed and renal disease status. Patients with prior bleed or renal disease had a higher incidence of MB vs those without the condition respectively. Generally, no significant interactions were observed between treatment of apixaban, warfarin or LMWH with prior bleed or with renal disease on rVTE and MB (p for interaction >0.1).
CONCLUSION: Incidence of MB was higher among those with prior bleed or renal disease. Effects of treatment with apixaban, warfarin, or LMWH were generally consistent regardless of prior bleed or renal disease status.
Total network controllability analysis discovers explainable drugs for Covid-19 treatment
Authors: Wei Xinru , Pan Chunyu , Zhang Xizhe , Zhang Weixiong 
Published: July 2023

Abstract
Background
The active pursuit of network medicine for drug repurposing, particularly for combating Covid-19, has stimulated interest in the concept of structural control capability in cellular networks. We sought to extend this theory, focusing on the defense rather than control of the cell against viral infections. Accordingly, we extended structural controllability to total structural controllability and introduced the concept of control hubs. Perturbing any control hub may render the cell uncontrollable by exogenous stimuli like viral infections, so control hubs are ideal drug targets.
Results
We developed an efficient algorithm to identify all control hubs, applying it to the largest homogeneous network of human protein interactions, including interactions between human and SARS-CoV-2 proteins. Our method recognized 65 druggable control hubs with enriched antiviral functions. Utilizing these hubs, we categorized potential drugs into four groups: antiviral and anti-inflammatory agents, drugs acting on the central nervous system, dietary supplements, and compounds enhancing immunity. An exemplification of our approach's effectiveness, Fostamatinib, a drug initially developed for chronic immune thrombocytopenia, is now in clinical trials for treating Covid-19. Preclinical trial data demonstrated that Fostamatinib could reduce mortality rates, ICU stay length, and disease severity in Covid-19 patients.
Conclusions
Our findings confirm the efficacy of our novel strategy that leverages control hubs as drug targets. This approach provides insights into the molecular mechanisms of potential therapeutics for Covid-19, making it a valuable tool for interpretable drug discovery.
Description and genomic characterization of Cohnella amylolytica sp. nov., isolated from Hot Springs in Yunnan province, south-west China
Authors: Xiang Ming-Xian , Miao Cui-Ping , Wang Juan , Li Yi-Qing , Yin Min , Tang Shu-Kun 
Published: July 2023

Abstract
A bacterial strain, Gram staining positive, strictly aerobic, rod-shaped, motile bacterium with flagellum and endospore-forming, designated strain YIM B05605T, was isolated from soil sampled in Hamazui hot springs, Tengchong City, Yunnan province, China. Optimum growth for the strain occurred at pH 7.0 and 45 °C. The predominant menaquinone of strain YIM B05605T was MK-7. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), unidentified glycolipid (GL), three unknown aminophospholipids (APLs) and unidentified polarlipid (PL) were part of the polar lipid profle. The major fatty acids were anteiso-C15:0 and iso-C16:0. The DNA G+C content of the type strain was 58.76 mol%. Genome-based phylogenetic analysis confirmed that strain YIM B05605T formed a distinct phylogenetic cluster within the genus Cohnella. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain YIM B05605T with the most related species C. fontinalis YT-1101T were 73.42 % and 15.7 %. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain YIM B05605T could be classified as a novel species of the genus Cohnella, for which the name Cohnella amylolytica sp. nov., is proposed. The type strain is YIM B05605T (= CGMCC 1.60052 T= KCTC 43462 T= NBRC 115921 T).
Indirect estimation of distance of a star from apparent magnitude.
Authors: Adhikary Abisa Sinha , Chattopadhyay Tanuka , Chattopadhyay Asis Kumar 
Published: July 2023

Abstract
Space observatory Gaia has prepared a large catalog of 1 billion astronomical objects which consists primarily of stars but also planets, comets, asteroids and quasars among others whose parallaxes are measured instead of distances (r). Bailer-Jones (Bailer-Jones [1]) established that distance estimation from parallaxes is not trivial once the fractional parallax error is larger than about 20%, which will be the case for about 80% of stars in the Gaia catalog. In the present model the distance estimates are developed through a Bayesian model using apparent magnitudes which are distant dependent intrinsic properties of stars. The proposed model is based on apparent magnitude limited distribution (viz. φ(m)) constructed with the combination of the probability density function (pdf) of an absolute magnitude limited distribution Φ(M) along with a prior. The former one is constructed from real data set of Gaia Catalogue of apparent magnitudes, corrected for extinction and parallaxes of a huge number of stars. The posterior distribution of the estimated distances thus found has variances and bias under control even for a lager fractional error (viz. more than 80 % compared to the previous work ) and hence for a larger distance.
Airborne dust particles originated from sand and gravel quarries: Mineralogical, geochemical, and size distribution constraints on their potential health impacts
Authors: Menhaje-Bena Rabeah , Modabberi Soroush , Bakand Shahnaz , Kazemian Hossein , Khansari Mahmoud Ghazi , Koohi Mohammad Kazem 
Published: July 2023

Abstract
Dust particles derived from sand and gravel mining have been considered as one of the possible sources of suspended particles in Tehran, the capital city of Iran. In this research, the size, morphological, and geochemical characteristics of the airborne particles originated from open mines were investigated. Twenty-two samples from different heights (3 to 21m) were collected from a sand and gravel quarry in Shahriar as the representative of the numerous quarries in western Tehran. The selected samples were further analyzed using X-ray powder diffraction (XRD) and Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy (SEM-EDS). The main mineralogy of airborne dust was dominated by quartz, followed by albite and calcite. The size distribution of deposited particles at different heights ranged from 0.05 µm to 100 µm and about 80% of them were respirable (< 10 µm) and available for transfer through the atmosphere. Si/Al ratios fall mainly into a range between 4 and 10. About 80% of the total particles are below 10 µm. Most nanoparticles were settled into agglomerated forms.
The impacts of patient mistreatment on healthcare workers’ role behaviors: a study in Chinese Fangcang shelter hospitals
Authors: Yan Wei , Bao Na , Zheng Shuangjiang , Wang Huan , Yue Dongmei , Chen Li 
Published: July 2023

Abstract
Background: Fangcang shelter hospitals have played an important role in the battle against the COVID-19 epidemic in China. Verbal and physical attacks on patients are prone to occur in such hospitals. This study explored the impacts of patient mistreatment on healthcare workers’ role behaviors (service performance and patient-oriented organizational citizenship behavior).
Methods: We examined the influence of patient mistreatment on service performance and patient-oriented organizational citizenship behavior, as well as the mediating effect of emotional exhaustion and the moderating effect of displaced aggression by patients, using hierarchical linear regression and conditional process analysis.
Results: Patient mistreatment was positively associated with emotional exhaustion among healthcare workers, while emotional exhaustion was negatively associated with service performance and patient-oriented organizational citizenship behavior. Mediation analysis revealed that emotional exhaustion mediated the association between patient mistreatment and both types of role behaviors. Moderated mediation analysis found that the mediation effect was weaker when the displaced aggression by patients was high.
Conclusions: The findings clarified the relationship between patient mistreatment, emotional exhaustion, service performance, and patient-oriented organizational citizenship behavior. Additional assistance should be provided to healthcare workers dealing with patient mistreatment. Displaced aggression by patients attenuates the positive effects of patient mistreatment on the emotional exhaustion of healthcare workers. Our findings reveal the mechanism and boundary conditions of patient mistreatment affecting healthcare workers' service performance and patient-oriented organizational citizenship behavior.
Ethanol tolerance in engineered strains of Clostridium thermocellum
Authors: Olson Daniel G. , Maloney Marybeth , Lanahan Anthony , Cervenka Nicholas , Xia Ying , Pech-Canul Angel , Hon Shuen , Tian Liang , Ziegler Samantha J. , Bomble Yannick , Lynd Lee R. 
Published: July 2023

Abstract
Clostridium thermocellum is a natively cellulolytic bacterium that is promising candidate for cellulosic biofuel production, but the ethanol titers produced thus far are too low for commercial application. In several strains of C. thermocellum engineered for increased ethanol yield, ethanol titer seems to be limited by ethanol tolerance. Previous work to improve ethanol tolerance has focused on the WT organism. In this work, we focused on understanding ethanol tolerance in several engineered strains of C. thermocellum. We observed a tradeoff between ethanol tolerance and production. Adaptation for increased ethanol tolerance decreases ethanol production. Second, we observed a consistent genetic response to ethanol stress involving mutations at the AdhE locus. These mutations typically reduced NADH-linked ADH activity. About half of the ethanol tolerance phenotype could be attributed to the elimination of NADH-linked activity based on a targeted deletion of adhE. Finally, we observed that rich growth medium increases ethanol tolerance, but this effect is eliminated in an adhE deletion strain. Together, these suggest that ethanol inhibits growth and metabolism via a redox-imbalance mechanism. The improved understanding of mechanisms of ethanol tolerance described here lays a foundation for developing strains of C. thermocellum with improved ethanol production.
Epigenetic reprogramming of Nannochloropsis oceanica in response to CO 2 fluctuated environment
Authors: Liu Danmei , Wei Li 
Published: July 2023

Abstract
Low CO2 could induce carbon concentrating mechanism (CCM) in majority of microalgae and CCM are regulated at RNA level are well known, however, epigenetic modifications and their potential regulation of the transcription of masked genes at the genome level in response to CO2 fluctuation remain unclear. Here we examine histone modifications and gene expression during CCM induction using ChIP-seq and mRNA-seq. Epigenetic regulation in response to CO2 fluctuation and epigenome-association with phenotypic plasticity of CCM are firstly uncovered in marine microalga Nannochloropsis oceanica IMET1. The result showed that lysine butyrylation (Kbu) and histone H3K9m2 modifications were present in N. oceanica IMET1. Moreover, Kbu positively regulated gene expression. In response to CO2 fluctuation, there were 5,438 and 1,106 genes regulated by Kbu and H3K9m2, respectively. Differential modifications were enriched in carbon fixation, photorespiration, photosynthesis, and lipid metabolism etc. For low carbon adaption, we observed that massively genome-wide epigenetic reprogramming would occur after N. oceanica cells shifted from high CO2 to low CO2. Gained or lost histone methylations were closely associated with activating or repressing gene expressions. Particularly, we firstly noted that the transcription of the key low CO2 responsive carbonic anhydrase (CA5), a key component involved in CCM stress signaling, was potentially regulated by bivalent Kbu-H3K9m2 modifications in microalgae. This study provides novel insights into epigenetic regulation in Nannochloropsis, which will lay foundation on genetic improvement of CCM at epigenetic level.
GWAS combined with transcriptome and metabolome to excavate key candidate genes for rice anaerobic germination
Authors: Li Dandan , Liu Kai , Zhao Chuanchao , Liang Siyi , Yang Jing , Peng Ziai , Xia Aoyun , Yang Meng , Luo Lixin , Huang Cuihong , Wang Jiafeng , Huang Ming , Xiao Wuming , Wang Hui , Su Ling , Guo Tao 
Published: July 2023

Abstract
In this study, a total of 317 SNP loci were detected based on dynamic genome-wide association study (GWAS) of the length (CL), surface area (CSA), volume (CV) and diameter (CD) of the coleoptile growing in 2d, 3d and 4d under anaerobic conditions in 591 natural rice populations. Integrated dynamic widely targeted metabolomic technology combined with liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) and data analysis, we found that xanthine, L-alanine and GABA may be key biomarkers that are sensitive and respond strongly to hypoxic stress perception. By combining targeted metabolomics and transcriptomics for WGCNA, we found that OsAlaAT1, OsGAD4, OsAAH and Os09g0424600 are the key structural genes that best characterize the metabolic processes of L-alanine, GABA and xanthine. Among them, OsAlaAT1 (Os10g0390500), located in Chr10-12877840, which is within the GWAS location range of CVAN3d, is considered to be a more reliable candidate gene. Through phenotype observation of OsAlaAT1 transgenic plants and qRT‒PCR analysis, it was found that the coleoptile length (CL) and relative expression level of the ko-alaat1 mutant were significantly decreased compared with the control (ZH11). GUS staining showed that OsAlaAT1 was strongly expressed in the coleoptile, embryo and radicle during seed germination under anaerobic conditions. Overall, in addition to providing new insight into the metabolic regulation of L-alanine, GABA and xanthine during hypoxic germination of rice, the outcome of our study establishes a foundation for anaerobic germination (AG) improvement in rice.
Utilization of electronic medical record system and associated factors among health professionals in public health facilities of Harari Region, Eastern Ethiopia
Authors: Mekonin Gediyon , Gobena Tesfaye , T/Mariyam Zelalem , Hawilte Behaylu , Kenay Abera 
Published: July 2023

Abstract
Background
Electronic medical record is computer-based patient recording system at the facility level and it’s a digital version of a paper chart in a clinical office. Moreover, an electronic record of health-related information, on an individual that can be created, gathered, managed, and consulted by authorized clinicians and staffs within one health care organization.
Objective
- To assess the level of utilization and determinants of electronic medical record use among health professionals working in public health facilities in Harari Regional State, Eastern Ethiopia
Methods
A facility- based cross-sectional study was conducted with 498 randomly selected health professionals at public health facilities of Harari Region. Pre-tested structured questionnaire that has been designed to be filled by the respondent which is used. Data collection facilitator participated in data collection. Data were entered into Epi Data Version 3.1 and exported to STATA version 14.2 for data processing and analysis. Descriptive statistics was used to describe the study variables. Bivariate and multivariate logistic regression analyses were used to identify the association b/n, between the outcome, and independent variables. A p-value < 0.05 at a 95% confidence level were considered as statistically significant.
Results
Of the total 551 approaching health professionals, 498(90.3%) were returned the questionnaire. Out of this, 211(42.3%, 95% CI: 38, 46) were using the electronic medical recording system. Females (AOR = 0.40, 95% CI: .251-.66), Health professionals with good Knowledge and Attitude and those having basic computer course had an association and a statistically significant result with utilization of EMR system ((AOR = 1.71, 95% CI: 1.07, 2.72), (AOR = 2.48, 95% CI: 1.53- 4.00), (AOR = 4.45, 95% CI: 2.17–9.10)) respectively.
Conclusion and Recommendations:
as this study shows, the utilization of electro medical record system was low. Health professionals with basic computer courses, having good knowledge and a good attitude, were found to use an electronic medical record system. Therefore, improving the knowledge and awareness of health professionals is important for scaling up the electronic medical record system used in facilities.
Cross-domain NER in Data-poor Scenarios for Human Mobility Knowledge
Authors: Jiang Yutong , Jin Fusheng , Chen Mengnan , Liu Guoming , Pang He , Yuan Ye 
Published: July 2023

Abstract
In recent years, the exploration of knowledge in large-scale human mobility has gained significant attention. In order to achieve a semantic understanding of human behavior and uncover patterns in large-scale human mobility, Named Entity Recognition (NER) is a crucial technology. The rapid advancements in IoT and CPS technologies have led to the collection of massive human mobility data from various sources. Therefore, there’s a need of Cross-domain NER which can transfer entity information from the source domain to automatically identify and classify entities in different target domain texts. In the situation of data-poor, how could we transfer human mobility knowledge over time and space is particularly significant, therefore this paper proposes an Adaptive TextSequence Enhancement Module (at-SAM) to help the model enhance the association between entities in sentences in data-poor target domains. This paper also proposes a Predicted Label-Guided Dual Sequence Aware Information Module (Dual-SAM) to improve the transferability of label information. Experiments were conducted in domains that contain hidden knowledge about human mobility,the results show that this method can transfer task knowledge between multiple different domains in data-poor scenarios and achieve the best performance to date.
How China Anesthesiologists document and communicate difficult airway management
Authors: Luo Xiang , Yang Dong , Deng Xiao-Ming , Wang Qian-Yu , Du Xi-Yu 
Published: July 2023

Abstract

Background
According to the Chinese Society of Anesthesiology, it is recommended that patients with difficult airways be documented and notified, which will provide healthcare professionals with a direct reference when managing airways. However, compliance with this initiative remains unclear. This study was conducted to investigate the current status and need for difficult airway notification at Plastic Surgery Hospital and to explore the factors contributing to noncompliance.
Methods
Anesthesiologists, surgeons, and patients in Plastic Surgery Hospital were administered separate questionnaires regarding notification of difficult airway management. Participants were surveyed regarding their attitudes and current practices regarding difficult airway notification. In addition, questions were asked regarding the barriers that contribute to noncompliance.
Results
A total of 632 valid responses were obtained and analyzed, giving a response rate of 99.21%. 399 patients (89.46%) felt it was very important for anesthesiologists to inform them about their difficult airway, and 91.03% felt it was very important for them to receive a letter of their airway assessment. However, twenty-two anesthesiologists (64.7%) reported verbally informing less than 50% of patients about their difficult airway after surgery, and only four anesthesiologists informed all patients they encountered. Most surgeons (91.22%) and anesthesiologists (91.18%) believe that it is vital to inform patients verbally, while 114 surgeons (77.03%) and 31 anesthesiologists (91.18%) believe that it is essential to complete a difficult airway notification alert. Among the factors causing noncompliance, 17 (34.69%) believed that absence of mandatory rules, 9 (18.37%) believed that increased workload, and 8 (16.33%) believed that notification methods were lacking.
Conclusions
The compliance to difficult airway notification remains low in Plastic Surgery Hospital despite the high incidence of difficult airways. Although anesthesiologists, surgeons, and patients are strongly in favor of it. Among the barriers to compliance were the absence of a well-developed notification system and a means of notification such as an alert form for difficult airways. This may spur the anesthesiology society to publish the notification system.