bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between blood eosinophil count and Duchenne muscular dystrophy severity and prognosis: a retrospective cohort study
Authors: Jiang Zhi , Liao Hongmei , Wu Liwen , Hu Wenjing , Yang Liming , Chen Bo , Ning Zeshu , Tang Jingwen , Xu Rong , Chen Mei , Guo Feng , Liu Shulei 
Published: July 2023

Abstract
Background
Duchenne muscular dystrophy (DMD) is a rare hereditary muscular disease. The role of eosinophils in DMD has not been clarified. This study aims to evaluate the association between peripheral blood eosinophil count and severity and prognosis of DMD.

Methods
A retrospective cohort study was performed for 145 DMD patients between January 2012 and December 2020. Clinical data of 150 healthy children were collected as a control group. Logistic regression and Cox regression analyses were used to explore the influences of eosinophil count on DMD severity and prognosis.

Results
Eosinophil count in DMD group was lower than the control group (Z = 2.163, P = 0.031). It was negatively correlated with Vignos scale score, Spearman correlation coefficient was p = 0.245, P = 0.040 (at admission),p = 0.137, P = 0.032 (at follow-up); was a protective factor for high Vignos scale score at admission [odds ratio (OR) = 0.038, 95%CI: 0.002–0.752, P = 0.032] and follow-up (OR = 0.033,95%CI: 0.001–0.121, P = 0.039). The Cox regression analysis indicated that elevated eosinophil count was correlated with better therapeutic efficacy for DMD patients [hazard ratio (HR) = 2.218, 95%CI: 1.154–3.924, P = 0.016].

Conclusion
Eosinophil count in peripheral blood was correlated with the severity of DMD. It could indicate the therapeutic efficacy and prognosis of DMD patients to a certain extent. Eosinophils may be a potentially valuable biomarker or therapeutic target for DMD.
Lynch-like syndrome with germline WRN mutation in Bulgarian patient with synchronous endometrial and ovarian cancer
Authors: Kamburova Zornitsa Bogomilova , Dimitrova Polina Damyanova , Dimitrova Diana Strateva , Kovacheva Katya Stefanova , Popovska Savelina Lubenova , Nikolova Slavena Enkova 
Published: July 2023

Abstract
Background
Synchronous endometrial and ovarian cancer (SEOC) accounts for 50–70% of all synchronous gynecology cancers in women. Approximately 14% of SEOC cases are caused by Lynch syndrome (LS). The widespread introduction of “universal screening” at LS (all cases with CRC and all EC cases diagnosed before age 60 should be tested for MMR deficiency) has led to an increasing number of suspected LS cases- MMR-deficient tumors without germline mutation in the MMR genes. These cases are attributed to the so-called Lynch-like syndrome (LLS).

Case presentation
We present a case of LLS with a detected germline, likely pathogenic variant in the WRN gene. The proband was a woman diagnosed with SEOC at the age of 51 years. Histology of both tumors (endometrium and ovary) was endometroid and showed loss of MLH1 and PMS protein expression. Genetic testing by next generation sequencing (NGS) detected a germline mutation (in the heterozygous state) in the WRN gene - c.4109del, p.(Asn1370ThrfsTer23) in the proband.

Conclusions
The presented case contributes to the etiology of LLS and confirms the need for specific genetic testing, together with genetic counseling, in hereditary cancer syndromes. The use of combined information from clinicians, pathologists, genetic counselors, and data from NGS testing for cancer predisposition, clinical surveillance, and follow-up management in women with gynecology cancers, especially SEOC, could be improved.
Squamata reptiles as a potential source of helminth infections when preyed on by companion animals
Authors: Carbonara Mariaelisa , Mendoza-Roldan Jairo Alfonso , Lia Riccardo Paolo , Annoscia Giada , Iatta Roberta , Varcasia Antonio , Conte Giuseppe , Benelli Giovanni , Otranto Domenico 
Published: July 2023

Abstract
Background
Squamate reptiles cohabiting with companion animals may represent a source of helminth infections, especially through predation by dogs and cats with an outdoor lifestyle.

Methods
In order to assess the role of reptiles as intermediate/paratenic hosts of trophically transmitted helminths, synanthropic reptiles (n = 245) captured from different ecological settings (i.e., households, dog shelters, urban, peri-urban and rural areas or natural parks) of southern Italy were examined for endoparasites. Parasitic cysts (i.e., larval forms of acanthocephalans, cestodes and nematodes) and free helminths (i.e., adult nematodes and digeneans) were morphologically and molecularly identified, and statistical analysis was carried out to evaluate the correlations between reptiles, infections, and ecological settings.

Results
Overall, 31% of reptiles were positive for at least one helminth, with Podarcis siculus (18.7%) and Tarentola mauritanica (8.1%) being the most frequently infected species. Among the parasites of medical interest, Joyeuxiella echinorhyncoides showed the highest prevalence (19.7%), followed by Diplopylidium acanthotetra (10.5%), Joyeuxiella pasqualei, Mesocestoides lineatus (5.6%) and Physaloptera sp. (3.9%). Macracanthorhynchus hirudinaceus was detected once. Podarcis siculus and T. mauritanica were associated with cestode infections.

Conclusions
The wide range of helminths detected here in reptiles living in sympatry with pets and the fact that many of these helminth species are parasitic and may infect companion animals (e.g., J. pasqualei, J. echinorhyncoides, D. acanthotetra, Physaloptera sp.) and humans (i.e., Macracanthorhynchushirudinaceus, Mesocestoideslineatus) indicate the potential health risk associated with pets preying on these small vertebrates. Our results indicate the need for complementary investigations of trophically transmitted parasites in dogs and cats living in sympatry with reptiles.

Graphical Abstract
Epidemiology of injuries and illnesses in elite wheelchair basketball players over a whole season – a prospective cohort study
Authors: Weith Moritz , Junge Astrid , Rolvien Tim , Kluge Sascha , Hollander Karsten 
Published: July 2023

Abstract
Background
Wheelchair basketball is an adaptation of pedestrian basketball and one of the most popular Paralympic sports worldwide. The epidemiology of health problems in wheelchair basketball has been prospectively studied only during the Paralympic Games, the 2018 World Championships, the 2021 South America Wheelchair Basketball Championship, and one season of two American intercollegiate wheelchair basketball teams. The objective of the study was to prospectively monitor and analyze the prevalence, incidence, burden, and characteristics of injuries and illnesses in a wheelchair basketball league during an entire season for the first time.

Methods
All players of the highest German wheelchair basketball league (Bundesliga) were invited to participate in the study. Included players completed the Oslo Sports Trauma Research Center Questionnaire once a week during the entire season 2020/21 to report health problems. Exposure was captured by self-reported training time and officially-recorded competition time.

Results
Sixty of 117 players (51%, 47 male, 13 female) of the national league participated with an average response of 93%. Seventy health problems (5.5/1000 exposure hours [95% CI: 4.9–6.1]) were reported, including 54 injuries and 16 illnesses. Prevalence of health problems was 60% (95% CI: 48–72). Most injuries affected the shoulder (32% of all injuries), cervical spine/neck (17%), and hand (13%). More overuse injuries (2.9/1000 exposure hours [95% CI: 2.5–3.3]) than acute injuries (1.3/1000 exposure hours [95% CI: 1.0-1.6]) occurred. Of all health problems, 53% were associated with time-loss. The incidences of all health problems, illnesses, injuries, and overuse injuries were higher in women than in men.

Conclusions
Characteristics and frequency of injuries and illnesses during wheelchair basketball season differed from those during major wheelchair basketball tournaments. The high proportion of overuse injuries and the higher injury rates in women should be regarded in the development of individualized prevention measures. Since results from previous studies during major tournaments are only partially comparable to wheelchair basketball league play, further studies should follow.
Pathogenesis of miR-155 on nonmodifiable and modifiable risk factors in Alzheimer’s disease
Authors: Liu Jia-jia , Long Yun-fan , Xu Peng , Guo Hai-dong , Cui Guo-hong 
Published: July 2023

AbstractAlzheimer’s disease (AD) is a common age-related neurodegenerative disease in the central nervous system and is the primary cause of dementia. It is clinically characterized by the memory impairment, aphasia, apraxia, agnosia, visuospatial and executive dysfunction, behavioral changes, and so on. Incidence of this disease was bound up with age, genetic factors, cardiovascular and cerebrovascular dysfunction, and other basic diseases, but the exact etiology has not been clarified. MicroRNAs (miRNAs) are small endogenous non-coding RNAs that were involved in the regulation of post-transcriptional gene expression. miRNAs have been extensively studied as noninvasive potential biomarkers for disease due to their relative stability in bodily fluids. In addition, they play a significant role in the physiological and pathological processes of various neurological disorders, including stroke, AD, and Parkinson’s disease. MiR-155, as an important pro-inflammatory mediator of neuroinflammation, was reported to participate in the progression of β-amyloid peptide and tau via regulating immunity and inflammation. In this review, we put emphasis on the effects of miR-155 on AD and explore the underlying biological mechanisms which could provide a novel approach for diagnosis and treatment of AD.
Acupuncture methods for insomnia disorder in the elderly: protocol for a systematic review and network meta-analysis
Authors: Dong Weitao , Zhou Hao , Wu Rong , He Ximeng , Chen Xingliang , Zhou Hongchi , Gong Tingting , Wang Chao 
Published: July 2023

Abstract
Background
Insomnia disorder remains one of the most common sleep disorders in the elderly, with high prevalence and substantial consequences for patients’ general health. Despite that increasing clinical trials have indicated that acupuncture seems to be effective for insomnia disorder in the elderly, comparative efficacy and safety of different acupuncture methods for elderly individuals with insomnia disorder has been unclear. Therefore, this protocol outlined a plan to evaluate and rank the efficacy and safety of various acupuncture approaches for insomnia disorder in the elderly.

Methods
A systematic search of 8 bibliographic databases will be conducted from their inception to 18 June 2023, including Cochrane Library, MEDLINE (via PubMed), Embase, Web of Science, Chinese National Knowledge Infrastructure (CNKI), Wanfang Database, VIP Database, and Chinese Biomedical Literature Database (CBM). Randomized controlled trials investigating acupuncture methods for insomnia disorder in the elderly, published in English or Chinese will be included. The primary outcome is sleep quality measured by the Pittsburgh Sleep Quality Index (PSQI). Two reviewers will independently perform study selection, data extraction and risk assessment of bias. The quality of included literatures will be appraised using Cochrane risk-of-bias tool (ROB 2.0). ADDIS (Aggregate Data Drug Information System) V.1.16.8 will be used to conduct Bayesian network meta-analysis. The quality of evidence will be evaluated using the Grading of Recommendations Assessment, Development and Evaluation System (GRADE).

Discussion
In this study, the results will provide credible evidence to assess the efficacy and safety of acupuncture therapies for elderly patients with insomnia disorder, assisting patients, physicians and clinical research investigators to select the most appropriate acupuncture method.

Systematic review registration
The protocol has been registered at OSF (https://osf.io/3kjpq/) with a registration number https://doi.org/10.17605/OSF.IO/3KJPQ.
The impact of influenza and pneumococcal vaccination on antibiotic use: an updated systematic review and meta-analysis
Authors: van Heuvel Lotte , Paget John , Dückers Michel , Caini Saverio 
Published: July 2023

Abstract
Background
Vaccination can prevent bacterial and viral infections that could otherwise increase the chances of receiving (unnecessary) antibiotic treatment(s). As a result, vaccination may provide an important public health intervention to control antimicrobial resistance (AMR).

Objectives
Perform a systematic literature review to better understand the impact of influenza, pneumococcal and COVID-19 vaccination on antibiotic use, and to identify differences in effect between world regions and study designs.

Methods
We performed a systematic literature review and meta-analysis which updated previous literature reviews with new data from 1 October 2018 to 1 December 2021. The study focuses on randomised controlled trials (RCTs) and observational studies. Results from the meta-analysis of RCTs were stratified by WHO region and age group. Vote counting based on the direction of effect was applied to synthesize the results of the observational studies.

Results
Most studies are performed in the WHO European Region and the Region of the Americas in high-income countries. RCTs show that the effect of influenza vaccination on the number of antibiotic prescriptions or days of antibiotic use (Ratio of Means (RoM) 0.71, 95% CI 0.62–0.83) is stronger compared to the effect of pneumococcal vaccination (RoM 0.92, 95% CI 0.85–1.00). These studies also confirm a reduction in the proportion of people receiving antibiotics after influenza vaccination (Risk Ratio (RR) 0.63, 95% CI 0.51–0.79). The effect of influenza vaccination in the European and American regions ranged from RoM 0.63 and 0.87 to RR 0.70 and 0.66, respectively. The evidence from observational studies supports these findings but presents a less consistent picture. No COVID-19 studies were identified.

Conclusion
We find that both RCTs and observational studies show that influenza vaccination significantly reduces antibiotic use, while the effect of pneumococcal vaccination is less pronounced. We were unable to study the effect of COVID-19 vaccination and no clear regional patterns were found due to the high heterogeneity between studies. Overall, our data supports the use of influenza vaccination as an important public health intervention to reduce antibiotic use and possibly control AMR.
Subjective and Performance-Based Cognition and Their Associations with Head Injury History in Older Former National Football League Players
Authors: ALLEN ANDREW T. , COLE WESLEY R. , WALTON SAMUEL R. , KERR ZACHARY YUKIO , CHANDRAN AVINASH , MANNIX REBEKAH , GUSKIEWICZ KEVIN M. , MEEHAN WILLIAM P. , ECHEMENDIA RUBEN J. , MCCREA MICHAEL A. , BRETT BENJAMIN L. 
Published: July 2023

ABSTRACT

Purpose
Investigate the association between self-reported subjective and performance-based cognition among older (50–70 years) former professional American football players, as well as the relationship of cognitive measures with concussion history and years of football participation, as a proxy for repetitive head impact exposure.

Methods
Among older former National Football League (NFL) players (N = 172; mean age = 60.69 ± 5.64), associations of subjective (Patient Reported Outcome Measurement Information System Cognitive Function-Short Form) and performance-based cognitive measures (Brief Test of Adult Cognition by Telephone [BTACT] Executive Function and Episodic Memory indices) were assessed via univariable and multivariable regression models, with a priori covariates of depression and race. A similar univariate and multivariable regression approach assessed associations between concussion history and years of football participation with subjective and performance-based cognitive measures. In a sample subset (n = 114), stability of subjective cognitive rating was assessed via partial correlation.

Results
Subjective ratings of cognition were significantly associated with performance-based assessment, with moderate effect sizes (episodic memory η
p
2 = 0.12; executive function η
p
2 = 0.178). These associations were weakened, but remained significant (Ps < 0.05), with the inclusion of covariates. Greater concussion history was associated with lower subjective cognitive function (η
p
2 = 0.114, P < 0.001), but not performance-based cognition. The strength of association between concussion history and subjective cognition was substantially weakened with inclusion of covariates (η
p
2 = 0.057). Years of participation were not associated with measures of subjective or objective cognition (Ps > 0.05).

Conclusions
These findings reinforce the importance of comprehensive evaluation reflecting both subjective and objective measures of cognition, as well as the consideration of patient-specific factors, as part of a comprehensive neurobehavioral and health assessment of older former contact sport athletes.
FARnet: Farming Action Recognition From Videos Based on Coordinate Attention and YOLOv7-tiny Network in Aquaculture
Authors: yang xinting , Pan Liang , Wang Dinghong , Zeng Yuhao , Zhu Wentao , Jiao Dongxiang , Sun Zhenlong , Sun Chuanheng , Zhou Chao 
Published: July 2023

Highlights

The automatic detection and recognition of farming action in video are realized.

The YOLOv7-tiny was enhanced by incorporating Coordinate Attention (CA).

The performance indices mAP@.5 and mAP@.5:.95 improved by 0.1% and 6.6%, respectively.

An intelligent method for detecting "inspection" and "applying pesticides" is provided.

Abstract. In aquaculture, regular "inspection" and "applying pesticides" are essential to improving production efficiency and fish disease treatment, but the current aquaculture system does not effectively support these strategies. Therefore, this paper proposes a farming action recognition network (FARnet), which can accurately locate the farmers in the video and detect the actions of “applying pesticides” and “inspection.” The dataset was captured and produced by multi-angle cameras, which were consulted with relevant experts. In this network, Coordinate Attention (CA) was used to improve the Efficient Layer Aggregation Networks-tiny (ELAN-tiny) and Spatial Pyramid Pooling (SPP) structures in the YOLOv7-tiny network. The precise implementation methods are as follows: (1) The convolution in ELAN-tiny was replaced with the CA module, and a shortcut was added. (2) A CA module was added to the final layer of the Spatial Pyramid Pooling (SPP) module. (3) The improved Efficient Layer Aggregation Networks-Coordinate Attention (ELAN-CA) and Spatial Pyramid Pooling-Coordinate Attention (SPP-CA) were used to extract action features and perform feature correction by ADD (Feature fusion by feature map summation) in the backbone. The results demonstrated that the FARnet achieved significantly better detection results than the YOLOv7-tiny network, where mAP@.5 improved by 0.1% from 99.4% to 99.5%, and the mAP@.5:.95 improved by 6.6% from 78.2% to 84.8%. Therefore, the FARnet can effectively detect and identify the “inspection” and “applying pesticides” actions of farmers and provide useful input information for the intelligent management system. Keywords: Action detection, Applying pesticides, Coordinate attention, FARnet, Inspection.
Multi-frequency Doppler-free spectroscopy of cesium using an external cavity diode laser
Authors: Chang Pengyuan , Liu Jingyang , Zhou Xingyu , Li Jian , Wang Xiaomeng , Chen Jingbiao , Wang Qin 
Published: July 2023

We employed a different approach to develop multi-frequency saturated-absorption spectroscopy (SAS) involving both cesium hyperfine ground state levels using a multimode external cavity diode laser (ECDL), which could operate with neither another independent laser nor a modulator. The multi-frequency SAS is formed by atomic velocity groups on resonance with both of the laser modes from an ECDL in multimode operation, which are counterpropagated through the vapor cell as a quasicoherent pair of laser beams. A sign reversal of the sub-Doppler resonance under special pump–probe polarization with and without applied magnetic fields is observed. Simultaneously, the optical microwave generation of the multimode ECDL is also investigated experimentally. The free-running linewidth of the beat note spectra between two modes is about 475 Hz, which indicates a high coherence between them. This oscillator- and modulator-free approach provides a complementary scheme for existing optical microwave generation and has potential for improvements.
The impact of a combined TB/HIV intervention on the incidence of TB infection among adolescents and young adults in the HPTN 071 (PopART) trial communities in Zambia and South Africa
Authors: Shanaube Kwame , Schaap Ab , Mureithi Linda , Amofa-Sekyi Modupe , Paulsen Robynn , Cheeba Maina , Kangololo Bxyn , Vermaak Redwaan , Sisam Carmen , Kosloff Barry , de Haas Petra , Fidler Sarah , Ruperez Maria , Hayes Richard , Floyd Sian , Ayles Helen ,   on behalf of the TREATS Study Team
Published: July 2023

Background
HPTN071 (PopART) was a cluster randomized trial conducted in Zambian and South African (SA) communities, between 2013–2018. The PopART intervention (universal HIV-testing and treatment (UTT) combined with population-level TB symptom screening) was implemented in 14 communities. The TREATS study (2017–2021) was conducted to evaluate the impact of the PopART intervention on TB outcomes. We report on the impact of the combined TB/HIV intervention on the incidence of TB infection in a cohort of adolescents and young adults (AYA) aged 15–24 years.

Methods
A random sample of AYA was enrolled between July 2018 and July 2019 in 7 intervention vs 7 standard-of-care communities. We collected questionnaire data on risk factors for TB, and blood for measuring TB infection using QuantiFERON (QFT) Plus. AYA were seen at months 12 and 24 with all procedures repeated. Primary outcome was incidence of TB infection comparing intervention and standard-of-care communities. An incident case was defined as a participant with QFT interferon-gamma response of < 0.2 IU/ml plasma (‘negative’) at baseline and a QFT interferon-gamma response of > = 0.7 IU/ml (‘positive’) at follow up.

Results
We enrolled 4,648 AYA, 2,223 (47.8%) had a negative QFT-plus result at baseline, 1,902 (85.6%) had a follow up blood sample taken at 12 months or 24 months. Among the 1,902 AYA, followed for 2,987 person-years, 213 had incident TB infection giving (7.1 per 100 person-years). TB infection incidence rates were 8.7 per 100 person-years in intervention communities compared to 6.0 per 100 person-years in standard-of-care communities. There was no evidence the intervention reduced the transmission of TB (incidence-rate-ratio of 1.45, 95%CI 0.97–2.15, p = 0.063).

Conclusion
In our trial setting, we found no evidence that UTT combined with TB active case finding reduced the incidence of TB infection at population level. Our data will inform future modelling work to better understand the population level dynamics of HIV and TB.