bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract C023: Aryl hydrocarbon receptor and leptin promote prostate cancer progression and may be associated with chemoresistance
Authors: Khamit-Kush Kofi K. , Powell Joann B. , Handy Jeffrey A. , Bowen Nathan J. , Odero-Marah Valerie , Wu Daqing 
Published: January 2023

Abstract
The aryl hydrocarbon receptor (AhR) is a nuclear transcription factor and xenobiotic sensor reported to mediate diet-induced obesity and is upregulated in high-grade prostate cancer (PCa). Previously published data from our lab shows AhR protein is overexpressed and constitutively active in castration-resistant PCa cells. AhR signaling is promoted by high fat diet-induced obesity, when circulating leptin levels are also high. Physiologically relevant leptin concentration is proportional to BMI and is secreted by adipose cells to inhibit hunger under normal circumstances. Our preliminary results suggest that both AhR and leptin independently desensitize PCa cells to clinically-relevant chemotherapeutics however, the underlying mechanisms must be elucidated and may require sustained AhR signaling. We also conducted an integrative bioinformatics analysis to explore the role of AHR and LEPR in PCa, which revealed AHR and LEPR mRNA expression positively correlates in prostate cancer (P&lt;0.05). The mRNA expression levels of AHR and LEPR in PCa tissue samples (i.e. solid tissue normal samples from individuals with cancer in TCGA, N=498) were analyzed using the cBioPortal and Xena online tools. We will further investigate if the decrease in drug response is exacerbated in the presence of both leptin and AhR. Further understanding of the potential interactions between diet-induced obesity and chemoresistance can promote improved treatments for patients.
Citation Format: Kofi K. Khamit-Kush, Joann B. Powell, Jeffrey A. Handy, Nathan J. Bowen, Valerie Odero-Marah, Daqing Wu. Aryl hydrocarbon receptor and leptin promote prostate cancer progression and may be associated with chemoresistance [abstract]. In: Proceedings of the 15th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2022 Sep 16-19; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2022;31(1 Suppl):Abstract nr C023.
Abstract C055: CARM1-mediated BAF155 methylation promotes metastasis and is targetable to reduce disparate mortality in triple negative breast cancer
Authors: Kim Eui-Jun , Liu Peng , Wang Yidan , Ong Irene M. , Xu Wei 
Published: January 2023

Abstract
Breast cancer (BC) has disparate mortality rates between African American (AA) and non-Hispanic White (White) individuals. Despite an overall lower incidence of BC, AA women are 41% more likely to die from BC than White women. BC can be biochemically classified based on the expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), or classified by gene expression profiling as luminal, HER2+ and basal-like types. A recent large cohort analysis of nearly 200,000 patients found that AA women had a nearly three-fold increased risk of triple-negative BC, a subtype of basal-like BC that is associated with poor prognosis and metastasis. Although an increasing number of studies compare breast cancer molecular features and gene expression differences between breast tumors from AA and White patients, the underlying biological mechanisms contributing to this disparity are still unknown. We studied the oncogenic roles of coactivator associated arginine methyltransferase 1 (CARM1) and that is highly expressed in TNBC and CARM1 was overexpressed in AA patients through TCGA analysis. We have identified BRG1-Associated Factor 155 (BAF155), a component of SWI/SNF chromatin remodeling complex, is specifically methylated by CARM1. Herein, we discovered the dual roles of methylated-BAF155 (me-BAF155) in promoting tumor metastasis: activation of super-enhancer-addicted oncogenes by recruiting BRD4, and repression of interferon α/γ pathway genes to suppress host immune response. Pharmacological inhibition of CARM1 and BAF155 methylation not only abrogated the expression of an array of oncogenes, but also boosted host immune responses by enhancing the activity and tumor infiltration of cytotoxic T cells. Moreover, strong me-BAF155 staining was detected in circulating tumor cells from metastatic cancer patients. Despite low cytotoxicity, CARM1 inhibitors strongly inhibited TNBC cell migration in vitro, and growth and metastasis in vivo. These data suggest that me-BAF155 is a marker for detecting metastasis patients in AA women and CARM1 is an important epigenetic target for treatment of TNBC to reduce mortality in AA women.
Citation Format: Eui-Jun Kim, Peng Liu, Yidan Wang, Irene M. Ong, Wei Xu. CARM1-mediated BAF155 methylation promotes metastasis and is targetable to reduce disparate mortality in triple negative breast cancer [abstract]. In: Proceedings of the 15th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2022 Sep 16-19; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2022;31(1 Suppl):Abstract nr C055.
Abstract C114: Racial and ethnic differences in breast cancer incidence, clinicopathological features and survival by age and breast cancer subtypes
Authors: Colak Dilek , Al-Harazi Olfat , Khalil Hala , Aleid Maha M. , Almadouj Amal N. , Qanbar Sukina , Ajarim Dahish S. , Kaya Ibrahim H. , Al-Zahrani Ali S. 
Published: January 2023

Abstract
Background: Age and ethnicity are important factors in breast cancer (BC) disparities and outcome. However, studies examining both racial and age-related disparities are scarce. We sought to determine differences in epidemiological and clinicopathological characteristics of breast cancer between races in age-specific cohorts. Methods: Gulf Centre for Cancer Registration database and King Faisal Specialist Hospital and Research Center BC registry database for Saudi/Arab women (n=4,206) and Cancer Incidence in Five Continents Volume XI reports, and Surveillance, Epidemiology, and End Results for African-American and non-Hispanic White women with BC were used. Results: Early onset BC (age &lt; 50 years) was more prevalent among Arab women (63%) compared with African-American women (29%) and White-American women (21%). Young Arab and African-American women had more estrogen/progesterone receptor negativity than young White-American women. The triple negative and (HER2)+/(HR)– BC subtypes were more prevalent among young Arab women compared to African-American and White women. The Arab women had the worst prognosis, especially among young women cohort. The differences persisted even after stratification to aggressive BC subtypes. The age-standardized incidence rate was significantly higher in developed countries than in Arab countries within the elderly-women cohort, but was comparable in the young women cohort. Conclusions: While young Arab and Black women had significantly higher frequency of hormonal negativity than White women, Arab women showed significantly higher prevalence of HER2+/HR- and TNBC subtypes than Black and White women. The racial/ethnic differences were more pronounced in the young women cohort.
Citation Format: Dilek Colak, Olfat Al-Harazi, Hala Khalil, Maha M. Aleid, Amal N. Almadouj, Sukina Qanbar, Dahish S. Ajarim, Ibrahim H. Kaya, Ali S. Al-Zahrani. Racial and ethnic differences in breast cancer incidence, clinicopathological features and survival by age and breast cancer subtypes [abstract]. In: Proceedings of the 15th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2022 Sep 16-19; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2022;31(1 Suppl):Abstract nr C114.
Do Regions of Increased Inflammation Progress to New White Matter Hyperintensities?: A Longitudinal Positron Emission Tomography-Magnetic Resonance Imaging Study
Authors: Tozer Daniel J. , Brown Robin B. , Walsh Jessica , Hong Young T. , Williams Guy B. , O’Brien John T. , Aigbirhio Franklin I. , Fryer Tim D. , Markus Hugh S. 
Published: January 2023

Background:

Recent studies have demonstrated increased microglial activation using
11
C-PK11195 positron emission tomography imaging, indicating central nervous system inflammation, in cerebral small vessel disease. However, whether such areas of neuroinflammation progress to tissue damage is uncertain. We determined whether white matter destined to become white matter hyperintensities (WMH) at 1 year had evidence of altered inflammation at baseline.

Methods:

Forty subjects with small vessel disease (20 sporadic and 20 cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) and 20 controls were recruited to this case-control observational study from in- and out-patient clinics at Addenbrooke’s Hospital, Cambridge, UK and imaged at baseline with both
11
C-PK11195 positron emission tomography and magnetic resonance imaging; and magnetic resonance imaging including diffusion tensor imaging was repeated at 1 year. WMH were segmented at baseline and 1 year, and areas of new lesion identified. Baseline
11
C-PK11195 binding potential and diffusion tensor imaging parameters in these voxels, and normal appearing white matter, was measured.

Results:

Complete positron emission tomography-magnetic resonance imaging data was available for 17 controls, 16 sporadic small vessel disease, and 14 cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy participants.
11
C-PK11195 binding in voxels destined to become new WMH was lower than in normal appearing white matter, which did not progress to WMH (−0.133[±0.081] versus −0.045 [±0.044];
P
<0.001). Mean diffusivity was higher and mean fractional anisotropy lower in new WMH voxels than in normal appearing white matter (900 [±80]×10
−6
versus 1045 [±149]×10
−6
mm
2
/s and 0.37±0.05 versus 0.29±0.06, both
P
<0.001) consistent with new WMH showing tissue damage on diffusion tensor imaging a year prior to developing into new WMH; similar results were seen across the 3 groups.

Conclusions:
White matter tissue destined to develop into new WMH over the subsequent year is associated with both lower neuroinflammation, and white matter ultrastructural damage at baseline. Our results suggest that this tissue is already damaged 1 year prior to lesion formation. This may reflect that the role of neuroinflammation in the lesion development process occurs at an early stage, although more studies over a longer period would be needed to investigate this further.
Serum Tryptase in Allergic Rhinitis: Effect of Cetirizine Treatment
Authors: Bruno G. , Andreozzi P. , Magrini L. , Santangelo G. , Graf U. , Angelino A. 
Published: January 2023

Activated mast cells release a large range of potent mediators of allergic inflammation, including proteases. The tryptase serum levels were evaluated in 13 subjects suffering from allergic perennial rhinitis. Moreover, the effects of cetirizine treatment on serum tryptase were studied using the UniCap™ tryptase fluoroenzymeimmunoassay. In allergic patients the serum tryptase values (M±SD: 6.1 ± 2.4 μg/L) were significantly (p < 0.02) higher than the values detected in controls (3.0 ± 1.2 μg/L). In allergic rhinitis, after antihistamine treatment, tryptase values (4.4 ± 1.8 μg/L) decreased significantly (p < 0.001). After two weeks from the cetirizine stop, the tryptase levels increased again (5.5 ± 2.6 μg/l). The results demonstrate that mast cells are constantly activated in perennial allergic rhinitis. The antihistamine treatment is effective in reducing the tryptase release from mast cells, but the mechanism of action of cetirizine is still to understand.
GBCdb: RNA expression landscapes and ncRNA–mRNA interactions in gallbladder carcinoma
Authors: Guo Li , Xiang Yangyang , Dou Yuyang , Yin Zibo , Xu Xinru , Tang Lihua , Yu Jiafeng , Wang Jun , Liang Tingming 
Published: January 2023

AbstractGallbladder carcinoma (GBC), an aggressive malignant tumor of the biliary system, is characterized by high cellular heterogeneity and poor prognosis. Fewer data have been reported in GBC than other common cancer types. Multi-omics data will contribute to the understanding of the molecular mechanisms of cancer, cancer diagnosis and prognosis. Herein, to provide better understanding of the molecular events in GBC pathogenesis, we developed GBCdb (http://tmliang.cn/gbc/), a user-friendly interface for the query and browsing of GBC-associated genes and RNA interaction networks using published multi-omics data, which also included experimentally supported data from different molecular levels. GBCdb will help to elucidate the potential biological roles of different RNAs and allow for the exploration of RNA interactions in GBC. These resources will provide an opportunity for unraveling the potential molecular features of Gallbladder carcinoma.
Characterization of Danube Swabian population samples on a high-resolution genome-wide basis
Authors: Bánfai Zsolt , Kövesdi Erzsébet , Sümegi Katalin , Büki Gergely , Szabó András , Magyari Lili , Ádám Valerián , Pálos Ferenc , Miseta Attila , Kásler Miklós , Melegh Béla 
Published: January 2023

Abstract
Background
German-derived ethnicities are one of the largest ethnic groups in Hungary, dating back to the formation of the Kingdom of Hungary, which took place at the beginning of the 11th century. Germans arrived in Hungary in many waves. The most significant immigration wave took place following the collapse of the Ottoman Empire in East-Central Europe which closed the 150 year long Ottoman occupation. To date, there are no comprehensive genome-wide studies investigating the genetic makeup of the Danube Swabians. Here we analyzed 47 Danube Swabian samples collected from elderly Swabian individuals living in the Dunaszekcső-Bár area, in Danube side villages of Southwest Hungary. These Swabians, according to self-declaration, did not admix with other ethnic groups for 3–6 succeeding generations. Using Illumina Infinium 720 K Beadchip genotype data, we applied allele frequency-based and haplotype-based genome-wide marker data analyses to investigate the ancestry and genetic composition of the collected Danube Swabian samples.

Results
Haplotype-based analyses like identity by descent segment analysis show that the investigated Danube Swabians possess significant German and other West European ancestry, but their Hungarian ancestry is also prominent. Our results suggest that their main source of ancestry can be traced back to Western Europe, presumably to the region of Germany.

Conclusion
This is the first analysis of Danube Swabian population samples based on genome-wide autosomal data. Our results establish the basis for conducting further comprehensive research on Danube Swabians and on other German ethnicities of the Carpathian basin, which can help reconstruct their origin, and identify their major archaic genomic patterns.
Single‑pass four‑throw versus traditional knotting pupilloplasty for traumatic mydriasis combined with lens dislocation
Authors: Shen Chaolan , Liu Lingjuan , Su Ning , Cui Ling , Zhao Xin , Li Min , Zhong Haibin 
Published: January 2023

Abstract
Purpose
To compare the use of single‑pass four‑throw (SFT) and traditional double-pass two-throw knotting (DTT) techniques in pupilloplasty for traumatic mydriasis combined with lens dislocation, and to evaluate the learning curve between the two knotting techniques by wet lab.

Method
The eyes of 45 patients (45 eyes) were divided into two groups according to the knotting technique used: single‑pass four‑throw (22 eyes) or traditional double-pass-two-throw knotting (23 eyes). Combined phacoemulsification and pupilloplasty with pars plana vitrectomy were performed in traumatic mydriasis patients with lens dislocation. Preoperative and postoperative corrected distance visual acuity (CDVA), pupil diameter, intraocular pressure (IOP), pupilloplasty time, and complications were compared. Twenty ophthalmology residents were randomized to perform a pupilloplasty suturing exam with or without SFT knotting techniques in porcine eyes.

Result
All cases had a minimum follow‑up period of 6 months (range 6–12 months). There was no significant difference in the CDVA (P = 0.55), postoperative pupil diameter (P = 0.79), IOP (P > 0.05), anterior chamber exudate degree, and loosening or shedding of the line knot between the two groups. The duration of the pupilloplasty was 22.32 ± 4.58 min in the SFT group and 30.35 ± 5.55 min in the traditional group, which was a significant difference (P < 0.01). The residents in the SFT group had higher test scores and fewer surgical mistakes (P < 0.05).

Conclusion
The SFT knotting technique has a similar treatment effect and safety as the traditional technique but requires a shorter time and is easier to perform in pupilloplasty surgery.
Exploring the predictors of physical inactivity in a university setting
Authors: Ndupu Lawrence Bismarck , Faghy Mark , Staples Vicki , Lipka Sigrid , Bussell Chris 
Published: January 2023

Abstract
Background
Changes in lifestyle patterns and the dependence on technology have contributed to an increase in prevalence of inactivity. To address this there is a need to identify the predictors of physical inactivity using the Theoretical Domains Framework (TDF).

Methods
One hundred and twenty-one university administrative staff and 114 PhD students completed a survey. Physical activity (PA) levels were assessed using the Global Physical Activity Questionnaire (GPAQ), with participants scoring below 600 MET-minutes/week of total PA regarded as inactive. The predictors of physical inactivity were assessed using the Determinants of Physical Activity Questionnaire (DPAQ). Multiple regression analyses were used to identify which domains of the TDF predicted physical inactivity in the study samples.

Results
The results indicated that 64% of administrative staff (Mean = 411.3 ± 118.3 MET-minutes/week of total PA) and 62% of PhD students (Mean = 405.8 ± 111.0 MET-minutes/week of total PA) did not achieve the recommended PA levels. The physical skills domain (t 106 = 2.198, p = 0.030) was the significant predictor of physical inactivity amongst the administrative staff. Knowledge (t 99 = 2.018, p = .046) and intentions (t 99 = 4.240), p = 0.001) domains were the significant predictors of physical inactivity amongst PhD students.

Conclusions
The results of this study should be used as a theoretical starting point in carrying out behavioural diagnosis, which could inform the design of effective interventions to increase PA levels in universities and other settings.
Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model
Authors: Satitsri Saravut , Akrimajirachoote Nattaphong , Nunta Kanokkan , Ruennarong Nitwarat , Amnucksoradej Orawan , Muanprasat Chatchai 
Published: January 2023

AbstractPost-weaning diarrhea in piglets is a major problem, resulting in a significant loss in pig production. This study aimed to investigate the effects of piperine, an alkaloid abundantly found in black peppers, on biological activities related to the pathogenesis of post-weaning diarrhea using a porcine duodenal enteroid model, a newly established intestinal stem cell-derived in vitro model recapitulating physiology of porcine small intestinal epithelia. Porcine duodenal enteroid models were treated with disease-relevant pathological inducers with or without piperine (8 μg/mL and/or 20 μg/mL) before measurements of oxidative stress, mRNA, and protein expression of proinflammatory cytokines, nuclear factor-kappa B (NF-κB) nuclear translocation, barrier leakage, and fluid secretion. We found that piperine (20 μg/mL) inhibited H2O2-induced oxidative stress, TNF-α-induced mRNA, and protein expression of proinflammatory cytokines without affecting NF-κB nuclear translocation, and prevented TNF-α-induced barrier leakage in porcine duodenal enteroid monolayers. Importantly, piperine inhibited fluid secretion induced by both forskolin and heat-stable toxins (STa) in a three-dimensional model of porcine duodenal enteroids. Collectively, piperine possesses both anti-inflammatory and anti-secretory effects in porcine enteroid models. Further research and development of piperine may provide novel interventions for the treatment of post-weaning porcine diarrhea.
A de novo PAK1 likely pathogenic variant and a de novo terminal 1q microdeletion in a Chinese girl with global developmental delay, severe intellectual disability, and seizures
Authors: Zhuang Jianlong , Xie Meihua , Yao Jianfeng , Fu Wanyu , Zeng Shuhong , Jiang Yuying , Wang Yuanbai , Xie Yingjun , Wang Gaoxiong , Chen Chunnuan 
Published: January 2023

Abstract
Background
Pathogenic PAK1 variants were described to be causative of neurodevelopmental disorder with macrocephaly, seizures, and speech delay. Herein, we present a de novo PAK1 variant combine with a de novo terminal 1q microdeletion in a Chinese pediatric patient, aiming to provide more insights into the underlying genotype–phenotype relationship.

Methods
Enrolled in this study was a 6-year-old girl with clinical features of global developmental delay, severe intellectual disability, speech delay, and seizures from Quanzhou region of China. Karyotype and chromosomal microarray analysis (CMA) were performed to detect chromosome abnormalities in this family. Whole exome sequencing (WES) was performed to investigate additional genetic variants in this family.

Results
No chromosomal abnormalities were elicited from the entire family by karyotype analysis. Further familial CMA results revealed that the patient had a de novo 2.7-Mb microdeletion (arr[GRCh37] 1q44(246,454,321_249,224,684) × 1]) in 1q44 region, which contains 14 OMIM genes, but did not overlap the reported smallest region of overlap (SRO) responsible for the clinical features in 1q43q44 deletion syndrome. In addition, WES result demonstrated a de novo NM_002576: c.251C > G (p.T84R) variant in PAK1 gene in the patient, which was interpreted as a likely pathogenic variant.

Conclusion
In this study, we identify a novel PAK1 variant associated with a terminal 1q microdeletion in a patient with neurodevelopmental disorder. In addition, we believe that the main clinical features may ascribe to the pathogenic variant in PAK1 gene in the patient.