bluuebunny/crossref_metadata_2025_split huggingface dataset:
Late surgical ventricular septal defect closure in a low middle-income country setting: a case series
Authors: Kalezi Zawadi Edward , Majani Naizihijwa , Simbila Alphonce Nsabi , Mongella Stella , Sharau Godwin Godfrey , Nkya Deogratias , Kubhoja Sulende 
Published: June 2023

Abstract
Background
Ventricular septal defect (VSD) is the commonest type of congenital heart lesion accounting for up to 40% of congenital heart defects. Well timed VSD closures are reported to yield excellent long-term outcomes. Late surgical VSD closures, particularly from the developing countries, are infrequently reported.

Case presentation
We report three cases of African children aged between 13 and 14 years who had late VSD presentations. They reported complaints of growth failure and recurrent respiratory infections since early infancy which necessitated frequent visits to primary health care facilities. They were found to have large ventricular septal defects by thoracic echocardiography. Diagnostic cardiac catheterization was done to all three patients to rule out irreversible pulmonary hypertension. After promising cardiac catheterization findings, they all underwent successful surgical VSD repair with good early outcomes.

Conclusion
VSD surgical closure is ideal in children below 2 years, however, it can be done in children who present at advanced age despite being considered high risk patients. All three of our patients who presented late had successful surgical VSD repairs with promising immediate outcome. The role of genetics in the protection against developing irreversible pulmonary vascular disease in these patients is a possible area for future studies.
Prospective comparison of [18F]AlF-NOTA-octreotide PET/MRI to [68Ga]Ga-DOTATATE PET/CT in neuroendocrine tumor patients
Authors: Boeckxstaens Lennert , Pauwels Elin , Vandecaveye Vincent , Deckers Wies , Cleeren Frederik , Dekervel Jeroen , Vandamme Timon , Serdons Kim , Koole Michel , Bormans Guy , Laenen Annouschka , Clement Paul M. , Geboes Karen , Van Cutsem Eric , Nackaerts Kristiaan , Stroobants Sigrid , Verslype Chris , Van Laere Koen , Deroose Christophe M. 
Published: June 2023

Abstract
Background
Fluorine-18-labeled SSAs have the potential to become the next-generation tracer in SSTR-imaging in neuroendocrine tumor (NET) patients given their logistical advantages over the current gold standard gallium-68-labeled SSAs. In particular, [18F]AlF-OC has already shown excellent clinical performance. We demonstrated in our previous report from our prospective multicenter trial that [18F]AlF-OC PET/CT outperforms [68Ga]Ga-DOTA-SSA, but histological confirmation was lacking due to ethical and practical reasons. In this second arm, we therefore aimed to provide evidence that the vast majority of [18F]AlF-OC PET lesions are in fact true NET lesions by analyzing their MR characteristics on simultaneously acquired MRI. We had a special interest in lesions solely detected by [18F]AlF-OC (“incremental lesions”).

Methods
Ten patients with a histologically confirmed neuroendocrine tumor (NET) and a standard-of-care [68Ga]Ga-DOTATATE PET/CT, performed within 3 months, were prospectively included. Patients underwent a whole-body PET/MRI (TOF, 3 T, GE Signa), 2 hours after IV injection of 4 MBq/kg [18F]AlF-OC. Positive PET lesions were evaluated for a corresponding lesion on MRI. The diagnostic performance of both PET tracers was evaluated by determining the detection ratio (DR) for each scan and the differential detection ratio (DDR) per patient.

Results
In total, 195 unique lesions were detected: 167 with [68Ga]Ga-DOTATATE and 193 with [18F]AlF-OC. The DR for [18F]AlF-OC was 99.1% versus 91.4% for [68Ga]Ga-DOTATATE, significant for non-inferiority testing (p = 0.0001). Out of these 193 [18F]AlF-OC lesions, 96.2% were confirmed by MRI to be NET lesions. Thirty-three incremental lesions were identified by [18F]AlF-OC, of which 91% were confirmed by MRI and considered true positives.

Conclusion
The DR of [18F]AlF-OC was numerically higher and non-inferior to the DR of [68Ga]Ga-DOTATATE. [18F]AlF-OC lesions and especially incremental lesions were confirmed as true positives by MRI in more than 90% of lesions. Taken together, these data further validate [18F]AlF-OC as a new alternative for SSTR PET in clinical practice.
Trial registration ClinicalTrials.gov: NCT04552847. Registered 17 September 2020, https://beta.clinicaltrials.gov/study/NCT04552847
Regulation of chromatin organization during animal regeneration
Authors: Jia Xiaohui , Lin Weifeng , Wang Wei 
Published: June 2023

AbstractActivation of regeneration upon tissue damages requires the activation of many developmental genes responsible for cell proliferation, migration, differentiation, and tissue patterning. Ample evidence revealed that the regulation of chromatin organization functions as a crucial mechanism for establishing and maintaining cellular identity through precise control of gene transcription. The alteration of chromatin organization can lead to changes in chromatin accessibility and/or enhancer-promoter interactions. Like embryogenesis, each stage of tissue regeneration is accompanied by dynamic changes of chromatin organization in regeneration-responsive cells. In the past decade, many studies have been conducted to investigate the contribution of chromatin organization during regeneration in various tissues, organs, and organisms. A collection of chromatin regulators were demonstrated to play critical roles in regeneration. In this review, we will summarize the progress in the understanding of chromatin organization during regeneration in different research organisms and discuss potential common mechanisms responsible for the activation of regeneration response program.
Residual insecticide surface treatment for preventing malaria: a systematic review protocol
Authors: Munn Zachary , Stone Jennifer C. , Barker Timothy Hugh , Price Carrie , Pollock Danielle , Kabaghe Alinune Nathanael , Gimnig John E. , Stevenson Jennifer C. 
Published: June 2023

Abstract

Introduction
Malaria presents a significant global public health burden, although substantial progress has been made, with vector control initiatives such as indoor residual surface spraying with insecticides and insecticide-treated nets. There now exists many different approaches to apply residual insecticide to indoor and outdoor surfaces in malaria-endemic settings, although no comprehensive systematic reviews exist evaluating these interventions. This manuscript outlines the protocol for a systematic review which aims to synthesise the best available evidence regarding full or partial indoor or outdoor residual insecticide surface treatment for preventing malaria.

Methods and analysis
This review will comprehensively search the literature (both published and unpublished) for any studies investigating the effectiveness of residual insecticide surface treatment for malaria. Studies will be screened to meet the inclusion criteria by a minimum of two authors, followed by assessment of risk of bias (using appropriate risk-of-bias tools for randomised and non-randomised studies) and extraction of relevant information using structured forms by two independent authors. Meta-analysis will be carried out where possible for epidemiological outcomes such as malaria, anaemia, malaria-related mortality, all-cause mortality and adverse effects. Certainty in the evidence will be established with GRADE assessments.

Ethics and dissemination
A full review report will be submitted to the Vector Control & Insecticide Resistance Unit, Global Malaria Program, WHO. A version of this report will be submitted for publication in an open access peer-reviewed journal. The report will inform the development of WHO recommendations regarding residual insecticide treatment for malaria. This systematic review does not require ethics approval as it is a review of primary studies.

Systematic review registration
PROSPERO 293194.
Parametric quintic spline for time fractional Burger’s and coupled Burgers’ equations
Authors: Hammad D. A. , Semary Mourad S. , Khattab Ahmed G. 
Published: June 2023

AbstractIn this paper, the numerical solutions of time fractional Burger’s and coupled Burgers’ equations are obtained using the parametric quintic spline method with a local truncation error of order eight in distance direction. Additionally, the von Neumann method was utilized for studying the stability analysis of the present method. Finally, to show the accuracy of this method, some examples with different cases for Burger’s and coupled Burgers’ equations are presented and their results are compared with the previous methods.
Low dose IV buprenorphine inductions for patients with opioid use disorder and concurrent pain: a retrospective case series
Authors: Murray John P. , Pucci Geoffrey , Weyer George , Ari Mim , Dickson Sarah , Kerins Angela 
Published: June 2023

Abstract
Background
Hospitalizations are a vital opportunity for the initiation of life-saving opioid agonist therapy (OAT) for patients with opioid use disorder. A novel approach to OAT initiation is the use of IV buprenorphine for low dose induction, which allows patients to immediately start buprenorphine at any point in a hospitalization without stopping full agonist opioids or experiencing significant withdrawal.

Methods
This is a retrospective case series of 33 patients with opioid use disorder concurrently treated with full agonist opioids for pain who voluntarily underwent low dose induction at a tertiary academic medical center. Low dose induction is the process of initiating very low doses of buprenorphine at fixed intervals with gradual dose increases in patients who recently received or are simultaneously treated with full opioid agonists. Our study reports one primary outcome: successful completion of the low dose induction (i.e. transitioned from low dose IV buprenorphine to sublingual buprenorphine-naloxone) and three secondary outcomes: discharge from the hospital with buprenorphine-naloxone prescription, self-reported pain scores, and nursing-assessed clinical opiate withdrawal scale (COWS) scores over a 6-day period, using descriptive statistics. COWS and pain scores were obtained from day 0 (prior to starting the low dose induction) to day 5 to assess the effect on withdrawal symptoms and pain control.

Results
Thirty patients completed the low dose induction (30/33, 90.9%). Thirty patients (30/33, 90.9%) were discharged with a buprenorphine prescription. Pain and COWS scores remained stable over the course of the study period. Mean COWS scores for all patients were 2.6 (SD 2.8) on day 0 and 1.6 (SD 2.6) on day 5. Mean pain scores for all patients were 4.4 (SD 2.1) on day 0 and 3.5 on day 5 (SD 2.1).

Conclusions
This study found that an IV buprenorphine low dose induction protocol was well-tolerated by a group of 33 hospitalized patients with opioid use disorder with co-occurring pain requiring full agonist opioid therapy. COWS and pain scores improved for the majority of patients. This is the first case series to report mean daily COWS and pain scores over an extended period throughout a low dose induction process.
Barriers to Third Mission: organizational and individual antecedents
Authors: Naranjo-Africano Gloria , Vega-Jurado Jaider , Manjarres-Henríquez Liney 
Published: June 2023

AbstractThe fulfillment of the University’s Third Mission has led the university to become a key actor for social and economic development in its regions of influence through a much closer linkage with the different agents in its environment. Thus, the literature has highlighted both the benefits derived from the Third University Mission, the difficulties inherent in the process and the existence of various barriers that can hinder its consolidation in the academic environment. Within the framework of these studies, it has been possible to identify three types of barriers to adopting the third mission: process, researcher, and ally or partner. It is worth mentioning that most of the research developed to date has focused on the study of the impact of the barriers on the development of the Third Mission but not on the analysis of the factors that can influence or attenuate the perception of these barriers. This is surprising if one considers that identifying these factors is relevant in designing strategies to promote the University’s Third Mission. Therefore, this article aims to identify the factors that influence or attenuate the researcher’s perception of different types of barriers to developing Third Mission activities. The study was based on data from a survey applied to a sample of academics categorized in the National System of Science, Technology and Innovation of Colombia and linked to 6 higher education institutions in the Colombian Caribbean region. Binary logistic regression models were established to analyze the data obtained. The results obtained suggest that, in addition to the researcher's previous experience in the external sector, their perception of the existence of processes or support services provided by the university for the promotion of relationships with actors in the socioeconomic environment is important to mitigate the barriers perceived during the development of Third Mission activities, especially when these activities are located in the support for the management and execution of technology transfer activities. In particular, it was found that as the academic's perception of the policies and procedures, support activities, and the university's technology transfer capacity improves, the barriers of both the researcher and those associated with the process are dissipated. This implies that universities should advance in the institutionalization of the third mission and in strengthening the functions of promotion, advice and support, as well as promotion structures and thus be able to mitigate the perceived barriers to the development of the University’s Third Mission.
Business resilience skills for SMEs
Authors: Kotsios Panagiotis 
Published: June 2023

AbstractThe goal of this research was to investigate the skills and values that can be related to building resilient Small and Medium Enterprises. Primary data on the topic were collected through survey research in a sample of 266 Greek and Polish business owners and managers during the summer of 2020. According to the replies, the Personal characteristics and Values category had the highest importance levels, but their adequacy levels were high as well. Especially Reliability, Integrity and Work ethics have been pointed out as vital for the long-term viability of a business while facing crises situations. The largest mismatch between importance and adequacy, by order of importance, were Communication, Risk identification and assessment, Financial Management, Planning and organisation and Customer-orientation, and these may constitute priority areas for inclusion in business training programs.
Characterization of metagenome-assembled genomes from the International Space Station
Authors: Singh Nitin K. , Wood Jason M. , Patane Jose , Moura Livia Maria Silva , Lombardino Jonathan , Setubal João Carlos , Venkateswaran Kasthuri 
Published: June 2023

Abstract
Background
Several investigations on the microbial diversity and functional properties of the International Space Station (ISS) environment were carried out to understand the influence of spaceflight conditions on the microbial population. However, metagenome-assembled genomes (MAGs) of ISS samples are yet to be generated and subjected to various genomic analyses, including phylogenetic affiliation, predicted functional pathways, antimicrobial resistance, and virulence characteristics.

Results
In total, 46 MAGs were assembled from 21 ISS environmental metagenomes, in which metaSPAdes yielded 20 MAGs and metaWRAP generated 26 MAGs. Among 46 MAGs retrieved, 18 bacterial species were identified, including one novel genus/species combination (Kalamiella piersonii) and one novel bacterial species (Methylobacterium ajmalii). In addition, four bins exhibited fungal genomes; this is the first-time fungal genomes were assembled from ISS metagenomes. Phylogenetic analyses of five bacterial species showed ISS-specific evolution. The genes pertaining to cell membranes, such as transmembrane transport, cell wall organization, and regulation of cell shape, were enriched. Variations in the antimicrobial-resistant (AMR) and virulence genes of the selected 20 MAGs were characterized to predict the ecology and evolution of biosafety level (BSL) 2 microorganisms in space. Since microbial virulence increases in microgravity, AMR gene sequences of MAGs were compared with genomes of respective ISS isolates and corresponding type strains. Among these 20 MAGs characterized, AMR genes were more prevalent in the Enterobacter bugandensis MAG, which has been predominantly isolated from clinical samples. MAGs were further used to analyze if genes involved in AMR and biofilm formation of viable microbes in ISS have variation due to generational evolution in microgravity and radiation pressure.

Conclusions
Comparative analyses of MAGs and whole-genome sequences of related ISS isolates and their type strains were characterized to understand the variation related to the microbial evolution under microgravity. The Pantoea/Kalamiella strains have the maximum single-nucleotide polymorphisms found within the ISS strains examined. This may suggest that Pantoea/Kalamiella strains are much more subjective to microgravity changes. The reconstructed genomes will enable researchers to study the evolution of genomes under microgravity and low-dose irradiation compared to the evolution of microbes here on Earth.
Skin microbiome differentiates into distinct cutotypes with unique metabolic functions upon exposure to polycyclic aromatic hydrocarbons
Authors: Leung Marcus H. Y. , Tong Xinzhao , Shen Zhiyong , Du Shicong , Bastien Philippe , Appenzeller Brice M. R. , Betts Richard J. , Mezzache Sakina , Bourokba Nasrine , Cavusoglu Nukhet , Aguilar Luc , Misra Namita , Clavaud Cécile , Lee Patrick K. H. 
Published: June 2023

AbstractBackgroundThe effects of air pollutants, particularly polycyclic aromatic hydrocarbons (PAHs), on the skin microbiome remain poorly understood. Thus, to better understand the interplay between air pollutants, microbiomes, and skin conditions, we applied metagenomics and metabolomics to analyze the effects of PAHs in air pollution on the skin microbiomes of over 120 subjects residing in two cities in China with different levels of air pollution.ResultsThe skin microbiomes differentiated into two cutotypes (termed 1 and 2) with distinct taxonomic, functional, resistome, and metabolite compositions as well as skin phenotypes that transcended geography and host factors. High PAH exposure was linked to dry skin and cutotype 2, which was enriched with species with potential biodegradation functions and had reduced correlation network structure integrity. The positive correlations identified between dominant taxa, key functional genes, and metabolites in the arginine biosynthesis pathway in cutotype 1 suggest that arginine from bacteria contributes to the synthesis of filaggrin-derived natural moisturizing factors (NMFs), which provide hydration for the skin, and could explain the normal skin phenotype observed. In contrast, no correlation with the arginine biosynthesis pathway was observed in cutotype 2, which indicates the limited hydration functions of NMFs and explains the observed dry skin phenotype. In addition to dryness, skin associated with cutotype 2 appeared prone to other adverse conditions such as inflammation.ConclusionsThis study revealed the roles of PAHs in driving skin microbiome differentiation into cutotypes that vary extensively in taxonomy and metabolic functions and may subsequently lead to variations in skin–microbe interactions that affect host skin health. An improved understanding of the roles of microbiomes on skin exposed to air pollutants can aid the development of strategies that harness microbes to prevent undesirable skin conditions.
Online multidisciplinary treatment of binge eating disorder in people with high weight: a case series study
Authors: Valdez-Aguilar Mariana , Vazquez-Arevalo Rosalia , López-Aguilar Xóchitl , Ruíz Martínez Ana Olivia , Rosinska Magda , Mancilla-Díaz Juan Manuel 
Published: June 2023

Abstract
Background
Binge eating disorder (BED) is the most frequent eating behavior among the general population (Guerdjikova in Med Clin 103:669–680, 2019). Many studies on interventions and BED treatments have been carried out in the United States and Europe, few have been reported in Latin American populations. People with this disorder not only have physical consequences of it but also social and psychological ones, therefore a multidisciplinary treatment approach is a good option to treat this condition.

Objective
To evaluate the feasibility of a multidisciplinary online intervention (i.e., psychological, nutritional, and physical activity) in patients with BED.

Method
The design was a case series study of two clinical treatment groups, with pre-test and post-test psychometric measures along with two follow-ups (at 2 and 6 months). Fifteen people diagnosed with BED (2 men and 13 women), with a mean age of 34.93 years (SD=11.91) and a mean initial BMI of 42, participated in this study. The treatment was carried out over the span of 28 sessions, each one being two hours per week consisting of 1 hour of group therapy and 1 hour of individual therapy. There were four evaluations: pre, post and two follow-ups.

Results
Five patients did not complete the treatment (30%). The comparisons were made through the non-parametric Friedman test, finding a statistically significant decrease in binge eating symptoms (x2=15.57; p=.001), anxiety symptoms (x2=15.96; p=.001) and depression (x2=15.03; p=.002). There was an improvement in clarity (x2=11.60; p=.010) and emotional regulation (x2=7.75; p=.050), only in women. The patients reduced their body weight, and improved their eating and exercise habits by introducing fruits and vegetables and including 20-30 minutes of physical activity into their daily routine. Regarding the Objective Clinical Change Index, in terms of the objective clinical change, a positive change was observed in all the variables addressed.

Conclusions
The data presented allowed us to conclude that the online multidisciplinary intervention was effective in the treatment of BED.
Trial registration Retrospectively registered
Feature selection of the respiratory microbiota associated with asthma
Authors: Salman Reem , Alzaatreh Ayman , Al Bataineh Mohammad T. 
Published: June 2023

AbstractThe expanding development of data mining and statistical learning techniques have enriched recent efforts to understand and identify metagenomics biomarkers in airways diseases. In contribution to the growing microbiota research in respiratory contexts, this study aims to characterize respiratory microbiota in asthmatic patients (pediatrics and adults) in comparison to healthy controls, to explore the potential of microbiota as a biomarker for asthma diagonosis and prediction. Analysis of 16 S-ribosomal RNA gene sequences reveals that respiratory microbial composition and diversity are significantly different between asthmatic and healthy subjects. Phylum Proteobacteria represented the predominant bacterial communities in asthmatic patients in comparison to healthy subjects. In contrast, a higher abundance of Moraxella and Alloiococcus was more prevalent in asthmatic patients compared to healthy controls. Using a machine learning approach, 57 microbial markers were identified and used to characterize notable microbiota composition differences between the groups. Among the selected OTUs, Moraxella and Corynebacterium genera were found to be more enriched on the pediatric asthmatics (p-values < 0.01). In the era of precision medicine, the discovery of the respiratory microbiota associated with asthma can lead to valuable applications for individualized asthma care.