bluuebunny/crossref_metadata_2025_split huggingface dataset:
An End-to-end Pipeline for Succinic Acid Production at an Industrially Relevant Scale usingIssatchenkia orientalis
Authors: Tran Vinh G. , Mishra Somesh , Bhagwat Sarang S. , Shafaei Saman , Shen Yihui , Allen Jayne L. , Crosly Benjamin A. , Tan Shih-I , Fatma Zia , Rabinowitz Joshua , Guest Jeremy S. , Singh Vijay , Zhao Huimin 
Published: May 2023

AbstractAs one of the top value-added chemicals, succinic acid has been the focus of numerous metabolic engineering campaigns since the 1990s. However, microbial production of succinic acid at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation. Here we describe the metabolic engineering ofIssatchenkia orientalis, a non-conventional yeast with superior tolerance to highly acidic conditions, for cost-effective succinic acid production. Through deletion of byproduct pathways, transport engineering, and expanding the substrate scope, the resulting strains could produce succinic acid at the highest titers in sugar-based media at low pH (pH 3) in fed-batch fermentations using bench-top reactors, i.e. 109.5 g/L in minimal medium and 104.6 g/L in sugarcane juice medium. We further performed batch fermentation in a pilot-scale fermenter with a scaling factor of 300×, achieving 63.1 g/L of succinic acid using sugarcane juice medium. A downstream processing comprising of two-stage vacuum distillation and crystallization enabled direct recovery of succinic acid, without further acidification of fermentation broth, with an overall yield of 64.0%. Finally, we simulated an end-to-end low-pH succinic acid production pipeline, and techno-economic analysis and life cycle assessment indicate our process is financially viable and can reduce life cycle greenhouse gas emissions by 34-90% relative to fossil-based production processes. We expectI. orientaliscan serve as a general industrial platform for the production of a wide variety of organic acids.
Chemotherapy-induced intestinal injury promotes Galectin-9-driven modulation of T cell function
Authors: Jansen Suze A. , Cutilli Alessandro , de Koning Coco , van Hoesel Marliek , Sierra Leire Saiz , Nierkens Stefan , Mokry Michal , Nieuwenhuis Edward E.S. , Hanash Alan M. , Mocholi Enric , Coffer Paul J. , Lindemans Caroline A. 
Published: May 2023

AbstractThe intestine is vulnerable to chemotherapy-induced toxicity due to its high epithelial proliferative rate, making gut toxicity an off-target effect in several cancer treatments, including conditioning regimens for allogeneic hematopoietic cell transplantation (allo-HCT). In allo-HCT, intestinal damage is an important factor in the development of Graft-versus-Host Disease (GVHD), an immune complication in which donor immune cells attack the recipient’s tissues. Here, we developed a novel human intestinal organoid-based 3D model system to study the direct effect of chemotherapy-induced intestinal epithelial damage on T cell behavior. Chemotherapy treatment using busulfan, fludarabine, and clofarabine led to damage responses in organoids resulting in increased T cell migration, activation, and proliferation in ex-vivoco-culture assays. We identified galectin-9 (Gal-9), a beta-galactoside-binding lectin released by damaged organoids, as a key molecule mediating T cell responses to damage. Increased levels of Gal-9 were also found in the plasma of allo-HCT patients who later developed acute GVHD, supporting the predictive value of the model system in the clinical setting. This study highlights the potential contribution of chemotherapy-induced epithelial damage to the pathogenesis of intestinal GVHD through direct effects on T cell activation and trafficking promoted by galectin-9.
A human omentum-specific mesothelial-like stromal population inhibits adipogenesis through IGFBP2 secretion
Authors: Ferrero Radiana , Rainer Pernille Yde , Russeil Julie , Zachara Magda , Pezoldt Joern , van Mierlo Guido , Gardeux Vincent , Saelens Wouter , Alpern Daniel , Favre Lucie , Mantziari Styliani , Zingg Tobias , Pitteloud Nelly , Suter Michel , Matter Maurice , Canto Carles , Deplancke Bart 
Published: May 2023

AbstractAdipose tissue plasticity is orchestrated by molecularly and functionally diverse cells within the stromal vascular fraction (SVF). While several mouse and human adipose SVF cellular subpopulations have now been identified, we still lack an understanding of the cellular and functional variability of adipose stem and progenitor cell (ASPC) populations across human fat depots. To address this, we performed single-cell and bulk RNA-seq analyses of >30 Lin–SVF samples across four human adipose depots, revealing two ubiquitous hASPC subpopulations with distinct proliferative and adipogenic properties but also depot- and BMI-dependent proportions. Furthermore, we identified an omental-specific, highIGFBP2-expressing stromal population that transitions between mesothelial and mesenchymal cell states and inhibits hASPC adipogenesis through IGFBP2 secretion. Our analyses highlight the molecular and cellular uniqueness of different adipose niches while our discovery of an anti-adipogenic IGFBP2+ omental-specific population provides a new rationale for the biomedically relevant, limited adipogenic capacity of omental hASPCs.
Play the game, live the story: pushing narrative boundaries with young adult videogames
Authors: Theodoulou Jack , Curwood Jen Scott 
Published: May 2023

PurposeVideogames are complex, meaningful and multimodal texts. This study aims to explore how students could learn about narratives from, and be engaged by, playing a videogame and how a teacher adapted their pedagogy to incorporate the young adult videogame (YA game)What Remains of Edith Finchinto an English Language Arts curriculum.Design/methodology/approachThis case study examined the experiences of a classroom teacher and students in a Year 10 English class in Australia. Thematic analysis included a wide range of data, including interviews, surveys, observations and artefacts.FindingsFirst, students demonstrated a strong understanding of the game as a narrative text, including essential components of narrative such as plot, characterisation, themes, settings and literary techniques. Second, students experienced a consistently high level of engagement and embodiment throughout the study as a consequence of the interactive, collaborative and multimodal nature of YA games. Third, the teacher discovered that he was able to achieve key curriculum outcomes with the videogame through re-imagining pedagogy.Originality/valueA playful approach allows teachers and students to be curious about the diverse narrative pathways possible within YA games and offers new opportunities to experience embodiment within and through digital texts.
The value relevance of accounting information: empirical evidence from Jordan
Authors: Abdelrahim Ahmad Khader Osama , Shareif Hussein Shanak Hosni 
Published: May 2023

Purpose
This empirical study’s primary goal is to examine the connection between accounting information and share price for financial companies listed on Jordan’s expanding Amman Stock Exchange (ASE) between 2014 and 2018.

Design/methodology/approach
The correlation between accounting data and share price was investigated using multiple regression analysis. In this vein, “pooled ordinary least squares (OLS), fixed effect and random effect” static panel data estimators were used. The OLS model was chosen as the best model after a series of diagnostic tests.

Findings
The multiple proxies of accounting information value relevance have a positive and considerable impact on the market value per share, according to panel data research. Comparatively, the authors find proof that among the other accounting data – earnings, dividends and cash flow from operations – book value is statistically the most value-relevant.

Research limitations/implications
This empirical investigation was only conducted in Jordan. Because it is very likely to obtain different results in other nations, the findings cannot be applied to other business environments.

Practical implications
The findings of this paper may highlight the amazing relationship between accounting information and share price for policymakers, regulators and other stakeholders in developing nations, notably in Jordan. This could pave the way for effective accounting disclosures.

Originality/value
Seldom does empirical research on the relationship between accounting data and share prices from publicly traded companies on ASE exist. So, by demonstrating empirical findings from Jordanian companies, this study fills the gap in the existing literature and knowledge.
Legality of export restrictions imposed during COVID-19 in international economic law
Authors: Gadkari Ahan 
Published: May 2023

PurposeThis paper aims to examine options under the General Agreement on Tariffs and Trade (GATT) for exempting or justifying export restrictions or prohibitions that are in principle prohibited under Article XI:1 GATT. The paper begins by examining the exception under Article XI:2 (a) GATT, before going on to the arguments under GATT Article XX (b) and (j). In addition, the analysis considers the national security exception in Article XXI (b) (iii) GATT, given that WTO members have increasingly invoked this provision in recent years, as well as during the pandemic, when Namibia implemented COVID-19-related trade restrictions under the Agreement on Technical Barriers to Trade based on national security concerns.Design/methodology/approachThe impacts of the COVID-19 pandemic on trade have been far-reaching. Countries have attempted to place export restrictions on personal protective equipment and COVID-19 vaccines. Even though export restrictions are generally unlawful under the GATT, countries have decided it is necessary at this time. Members have relied heavily on the “national security” and “critical shortage” exceptions outlined in the GATT.FindingsThis paper concludes that, depending on the circumstances of a particular case, a pandemic may constitute an emergency in international relations, as defined in Article XXI (b) (iii) GATT, and that, in such a situation, a WTO member may legitimately take action to protect its vital security interests.Originality/valueThe paper provides an original conclusion based on WTO case law on an issue of contemporary relevance.
Meaning in challenging times: how we might respond to the global COVID-19 pandemic?
Authors: Steger Michael F. , Sung Angelina , Dao Truc Anh L. , Tompkins Trudy M. 
Published: May 2023

Purpose
The purpose of this paper is to examine meaning in life as an important resource during the COVID-19 pandemic.

Design/methodology/approach
This paper summarized key research establishing links between meaning in life and mental health and well-being variables, reviewed the literature on meaning as a protective factor and meaning-making as a coping mechanism amidst the COVID-19 pandemic, as well as provided focal points in cultivating meaning in life.

Findings
Studies strongly support the notion that meaning in life is essential for health and well-being. Research also suggests that meaning protects against worsening mental health, and that engaging in meaning-making is a coping process that ultimately leads to improved adjustment despite the stressors of the COVID-19 pandemic. Eight themes are also identified in cultivating skills to build meaning during adverse times.

Practical implications
Integrating what research has found about meaning, resilience and coping can help individuals develop practical strategies to cultivate meaning in their lives to support themselves and their communities during stressful times.

Originality/value
Understanding the ways in which meaning can support individuals’ health and well-being is critical during a global upheaval such as that of the COVID-19 pandemic.
The credit cycle and corporate strategy: challenges and solutions
Authors: Calandro Jr. Joseph , Paharia Vivek 
Published: May 2023

Purpose
This paper offers a practical overview of the U.S. credit cycle and the challenges it poses, along with a perspective on where we seem to be in the cycle in early 2023. Suggestions are then offered for how corporate executives can address cyclical challenges from a corporate strategy perspective.

Design/methodology/approach
The United States credit cycle was out into context by following the trend of Moody’s Baa corporate bond yields from January 1919 to November 2022. Under the Moody’s rating system, Baa is the lowest level of investment grade credit, and as such it possesses speculative characteristics that are sensitive to cyclical dynamics. Another reason for choosing Baa credit patterns for analysis is data availability: over 100-years of continuous Baa data is searchable at the U.S. Federal Reserve.

Findings
The prior credit cycle wave of progressively lower inflation and interest rates began in 1982 and ended in 2020. The current credit cycle of wave of progressively higher inflation and interest rates will present strategic risks and opportunities that executives will increasingly have to deal with.

Originality/value
This is the first corporate strategy paper we are aware that practically addresses the credit cycle change. It is also the first paper we are aware that provides practical suggestions on how to address that change from a corporate strategy perspective.
A flexible pressure sensor with interference immunity capability
Authors: Liu Huan , Wang Rui , Wang Junyao , Chen Xingyu , Li Yunpeng , Cui Bowen , Lang Tianhong , Zhu Weihua 
Published: May 2023

Purpose
Flexible pressure sensor arrays have promising applications in analog haptics, reconfiguration of sensory functions, artificial intelligence, wearable devices and human-computer interaction. The force disturbance generated by the connecting material between the sensor array units will reduce the detection accuracy of the unit. The purpose of this paper is to propose a flexible pressure sensor with interference immunity capability. A C-type bridge flexible piezoelectric structure is used to improve the pressure perturbation. The interference immunity capability of the sensor has been improved.

Design/methodology/approach
In this paper, a C-type pressure sensor array structure by rapid injection moulding is manufactured through the positive piezoelectric effect of a piezoelectric material. The feasibility of C-type interference immunity structure in a flexible sensor array is verified by further analysis and experiment. A flexible pressure sensor array with C-type interference immunity structure has been proposed.

Findings
In this paper, we present the results of the perturbation experiment results of the C-type pressure sensor array, showing that the perturbation error is less than 8%. The test of the flexible sensor array show that the sensor can identify the curved angle of up to 120 °, and the output sensitivity of the sensor in the horizontal state reaches 0.12 V/N, and the sensor can withstand the pressure of 80 N. The flexible sensor can work stably in the stretch rate range of 0–8.6% and the stretch length range of 0–6 mm.

Originality/value
In this paper, C-type pressure sensor array structure is fabricated by rapid injection moulding for the first time. The research in this paper can effectively reduce the disturbance of input pressure on the sensor’s internal array and improve the output accuracy. The sensor can intuitively reflect the number of fingers sliding on the sensor by the order in which the maximum voltage appears. Due to the strong interference immunity capability and flexibility of the flexible sensor array mechanism, it has a broad application prospect in the practical fields of haptic simulation, perceptual function reconstruction, artificial intelligence, wearable devices and human–computer interaction.
The writing quality scale (WQS): A new tool to identify writing difficulties in students
Authors: Stuart N. J. , Barnett A. L. 
Published: May 2023

AbstractStudents in higher education (HE) are required to complete a variety of writing tasks for coursework and examinations. However, for some students writing presents a major challenge. In the UK, the availability of tools for specialist assessors to help identify difficulties with the quality of written composition is limited. The aim of this study was to develop a practical new tool, the Writing Quality Scale (WQS) for assessing writing quality in HE, that was both easy to use and did not require specialist or subject knowledge. The reliability and validity of the tool and its ability to identify students in HE who may need support were evaluated by examining scripts from 120 students (60 male) aged 17 to 25 years. The WQS was found to have good inter‐rater reliability and was sensitive enough to pick up age differences and differentiate between groups of students with and without dyslexia. The WQS will be a useful tool for specialist assessors in HE to help in the identification of those with poor writing quality and to understand more about the nature of their difficulties.
Analyzing the impact of polder‐type flood control pattern on river system's regulation and storage capacity under urbanization
Authors: Gao Yuqin , Wu Ming , Liu Yunping , Gao Li , Zhang Zhenxing 
Published: May 2023

AbstractThe rapid development of urbanization has led to changes in the morphology and structure of the river system, exacerbating flooding in the basin and seriously affecting the regulation and storage capacity of the river system. We propose the evaluation indexes of the regulation and storage capacity, establish a hydrological–hydrodynamic model to investigate the effects of the polder‐type flood control pattern on the regulation and storage capacity during urbanization. The results show that the river system's static regulation and storage capacity exhibits a decreasing trend during the urbanization process. The change in static regulation and storage capacity of low‐grade rivers has a more significant impact on the total regulation and static storage capacity. The construction of polders increases the flood pressure of the river system and reduces the dynamic regulation and storage capacity. The smaller the size of the flood, the more pronounced the effect. With the increase in flood‐protected areas, the dynamic regulation and storage capacity gradually decreases, and minor flooding is more sensitive to the number of polders.
Variance effective population size is affected by census size in sub‐structured populations
Authors: Ryman Nils , Laikre Linda , Hössjer Ola 
Published: May 2023

AbstractMeasurement of allele frequency shifts between temporally spaced samples has long been used for assessment of effective population size (Ne), and this ‘temporal method’ provides estimates of Ne referred to as variance effective size (NeV). We show that NeV of a local population that belongs to a sub‐structured population (a metapopulation) is determined not only by genetic drift and migration rate (m), but also by the census size (Nc). The realized NeV of a local population can either increase or decrease with increasing m, depending on the relationship between Ne and Nc in isolation. This is shown by explicit mathematical expressions for the factors affecting NeV derived for an island model of migration. We verify analytical results using high‐resolution computer simulations, and show that the phenomenon is not restricted to the island model migration pattern. The effect of Nc on the realized NeV of a local subpopulation is most pronounced at high migration rates. We show that Nc only affects local NeV, whereas NeV for the metapopulation as a whole, inbreeding (NeI), and linkage disequilibrium (NeLD) effective size are all independent of Nc. Our results provide a possible explanation to the large variation of Ne/Nc ratios reported in the literature, where Ne is frequently estimated by NeV. They are also important for the interpretation of empirical Ne estimates in genetic management where local NeV is often used as a substitute for inbreeding effective size, and we suggest an increased focus on metapopulation NeV as a proxy for NeI.
Blockade of Kv7 channels reverses the inhibitory effects of exchange protein directly activated by cAMP activation on purinergic contractions of the murine detrusor
Authors: Fong Zhihui , Lall Anshika , Mullins Nicolas D. , Santana L. Fernando , Hollywood Mark A. , Thornbury Keith D. , Sergeant Gerard P. 
Published: May 2023

AbstractPurinergic contractions of the detrusor are reduced by cAMP, but the underlying mechanisms are unclear. We examined the effects of BK and Kv7 channel modulators on purinergic contractions of the detrusor and tested if the inhibitory effects of activators of the cAMP effectors, PKA and EPAC, were reduced by blockade of BK or Kv7 channels. Purinergic contractions of the murine detrusor were induced by electric field stimulation (EFS) or application of the P2X receptor agonist α,β‐MeATP. EFS responses were inhibited by the L‐type Ca2+ channel blocker nifedipine, but not by the SERCA inhibitor CPA or the SOCE blocker GSK7975A. The Kv7 channel opener retigabine and BK channel activator compound X inhibited purinergic responses, while blockade of Kv7 or BK channels with XE991 or iberiotoxin, respectively, augmented these responses. Application of the EPAC activator 007‐AM or PKA activator 6‐MB‐cAMP inhibited EFS responses. These effects were unaffected by iberiotoxin; however, XE991 reduced the effects of 007‐AM, but not 6‐MB‐cAMP. Kv7.5 was the only Kv7 transcript detected in isolated detrusor myocytes. These data suggest that purinergic contractions of the detrusor are regulated by BK and Kv7 channels and the latter may also play a role in EPAC‐dependent inhibition of this activity.
Humour, empathic concern and perspective‐taking in children. Cartooning about social inequality
Authors: Pedrazzini Ana , Zinkgräf Constanza , Bugallo Lucía , D'Adamo Paola , Lozada Mariana 
Published: May 2023

AbstractThis research is motivated by two inter‐related arguments. Humour and cartooning in children are useful means to (1) address sensitive socio‐political issues and (2) foster empathic concern and perspective‐taking. Humorous cartoons and multimodal narratives were created by 10–13‐year olds in school workshops about social inequality and social empathy. Students made cartoons related to concrete situations of economic, gender, racial and ethnic inequality. Children showed empathic concern towards the victims depicted, evidenced by representing positive empathy between characters or by denouncing a lack of empathy. This research suggests that composing humorous multimodal narratives can favour immersive experiences, perspective‐taking, empathy and pro‐sociality.