bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of virtual tour on perioperative pediatric anxiety
Authors: Franco Castanys Teresa , Jiménez Carrión Anabel , Ródenas Gómez Frederic , Clemente García Sandra , Melero Mascaray Alícia , Janeiro Amela Marisa , Busquets Bonet Jordi 
Published: January 2023

AbstractBackgroundThere is a high incidence of perioperative anxiety in the pediatric population, with adverse side effects, such as emergency delirium and maladaptive postoperative behaviors.AimsThe study's objective was to compare the level of preoperative anxiety in children after standard preparation plus a virtual tour of the operating room vs. standard preparation alone.Patients/MethodsThis was a prospective single‐center, randomized, controlled, blinded trial with parallel assignment, registered as NCT04043663. Eligible subjects were healthy children (ASA I‐II) aged 4–12, scheduled for outpatient surgery. Five visits were conducted during the study, two at the hospital and three over the phone. Variables assessed were child's anxiety through the modified Yale Perioperative Anxiety Scale, demographic data, cooperation with induction through the Induction Compliance Checklist, preoperative parental anxiety through the State–Trait Anxiety Inventory and Anxiety Visual Analog Scale, the postoperative delirium degree through the Pediatric Anesthesia Emergence Delirium Scale, the presence of behavioral changes through the Post Hospitalization Behavior Questionnaire for Ambulatory Surgery, and the overall parental satisfaction.ResultsA total of 125 participants were included; 61 (48.8%) of them were randomized to the Virtual Tour Group (VT+) and 64 (51.2%) to the Non‐virtual Tour Group (VT‐). Yale Preoperative Anxiety Scale results in VT+ vs. VT‐ were mean 27.26 vs. 32.57, and median 23.4 (CI 95% 23.4–23.4) vs. 23.4 (CI 95% 23.4–33.4), (p = .0086). In the VT+ group, satisfaction was higher for questions one (p = .0213), three (p = <.0001), and four (p = .0130). Throughout the study, we observed a significant reduction in perioperative anxiety in the VT+ group, facilitating anesthetic induction in perfect (p = .018) and moderate compliance (p = .0428). The other variables did not show statistically significant differences.ConclusionOur study confirms previous studies that found virtual tours for perioperative patients may reduce perioperative anxiety and improve satisfaction. We found no impact on longer‐term outcomes.
Chromosome‐level wild Hevea brasiliensis genome provides new tools for genomic‐assisted breeding and valuable loci to elevate rubber yield
Authors: Cheng Han , Song Xiaoming , Hu Yanshi , Wu Tingkai , Yang Qihang , An Zewei , Feng Shuyan , Deng Zhi , Wu Wenguan , Zeng Xia , Tu Min , Wang Xiyin , Huang Huasun 
Published: January 2023

SummaryThe rubber tree (Hevea brasiliensis) is grown in tropical regions and is the major source of natural rubber. Using traditional breeding approaches, the latex yield has increased by sixfold in the last century. However, the underlying genetic basis of rubber yield improvement is largely unknown. Here, we present a high‐quality, chromosome‐level genome sequence of the wild rubber tree, the first report on selection signatures and a genome‐wide association study (GWAS) of its yield traits. Population genomic analysis revealed a moderate population divergence between the Wickham clones and wild accessions. Interestingly, it is suggestive that H. brasiliensis and six relatives of the Hevea genus might belong to the same species. The selective sweep analysis found 361 obvious signatures in the domesticated clones associated with 245 genes. In a 15‐year field trial, GWAS identified 155 marker–trait associations with latex yield, in which 326 candidate genes were found. Notably, six genes related to sugar transport and metabolism, and four genes related to ethylene biosynthesis and signalling are associated with latex yield. The homozygote frequencies of the causal nonsynonymous SNPs have been greatly increased under selection, which may have contributed to the fast latex yield improvement during the short domestication history. Our study provides insights into the genetic basis of the latex yield trait and has implications for genomic‐assisted breeding by offering valuable resources in this new domesticated crop.
Two for two: Dual therapy with erlotinib and acitretin for twins with severe keratoderma in Olmsted syndrome
Authors: Butala Sneha , Phan Sheshanna , Siegel Dawn H. , Carlberg Valerie , Paller Amy S. 
Published: January 2023

AbstractOlmsted syndrome (OS) is a rare genetic disorder, characterized by painful palmoplantar keratoderma (PPK), periorificial and intertriginous hyperkeratoses, and alopecia. Fewer than 75 cases have been described. Variants in TRPV3 result in constitutive activation of transient receptor potential vanilloid 3, leading to increased epidermal growth factor receptor (EGFR) signaling, palmoplantar epidermal hyperproliferation, and exquisite lesional pain. We describe pre‐school aged twins with OS with partial improvement from oral erlotinib, an EGFR inhibitor, but dramatic reduction of their persistent palmoplantar thickening and pain from adding acitretin.
Neural deficits in anticipatory and consummatory reward processing are uniquely associated with current depressive symptoms during adolescence
Authors: Thompson Brittney , Santopetro Nicholas J. , Brush Christopher J. , Foti Dan , Hajcak Greg 
Published: January 2023

AbstractDeficits within the consummatory phase of reward processing are associated with increased depression symptoms and risk; however, few studies have also examined other aspects of reward processing in relation to depression. In the current study, a community sample of 121 adolescents (Mage = 13.1, Min = 11.14; Max = 15.12; 54% male) completed self‐report questionnaires to assess depressive symptoms and the monetary incentive delay (MID) task while EEG was recorded. Results indicated that a reduced cue‐P300 as well as a reduced reward positivity (RewP) and feedback negativity (FN) to gain and loss feedback, respectively, were associated with increased depressive symptoms; on the other hand, SPN and feedback P300 were unrelated to depressive symptoms. An exploratory multiple regression analysis revealed that a reduced money cue‐P300, a reduced RewP, and a reduced (i.e., less negative) FN, all explained unique variance in depressive symptoms. The current study demonstrates that reduced cue‐P300, RewP, and FN amplitudes may reflect distinct deficits in reward processing among adolescents with increased depressive symptoms. Notably, this study is one of the first to leverage the MID task in adolescents in relation to depressive symptoms, allowing for a more in‐depth view of the individual differences in reward processing among adolescents with increased depressive symptomatology.
Effects of respiratory phases on the processing of emotional and non‐emotional visual stimuli
Authors: Mizuhara Keita , Nittono Hiroshi 
Published: January 2023

AbstractThe number of studies investigating the relationship between respiratory phases and cognitive/neural processing of external events has been increasing, but the findings remain controversial. This registered report examined the effect of the respiratory phase on the discrimination accuracy of visual stimuli in the emotional and non‐emotional domains. Forty‐two healthy young participants were asked to choose fearful over neutral facial expressions and to choose high‐contrast over low‐contrast Gabor patches during spontaneous nasal respiration. Event‐related potentials (ERPs) were also recorded for each type of stimulus presented during each respiratory phase. It was hypothesized that discrimination accuracy would be higher when the stimuli were presented during inhalation than during exhalation. It was also hypothesized that the amplitudes of ERPs elicited by the stimuli would be greater during inhalation than during exhalation. For comparison, the effect of the cardiac phase was examined, with the expectation that discrimination accuracy would be higher when the stimuli were presented during systole than during diastole. It was also hypothesized that the amplitudes of ERPs elicited by the stimuli would be greater during systole than during diastole. As expected, the results indicated that fear discrimination accuracy was higher during inhalation than exhalation and during systole than diastole. However, this was not the case for contrast discrimination. No differences in ERPs were observed between respiratory phases in either task. These results suggest that natural breathing in through the nose facilitates the discrimination of emotional stimuli, possibly via subcortical processes.
Parental affect profiles predict child emotion regulation and classroom adjustment in families experiencing homelessness
Authors: Labella Madelyn H. , Distefano Rebecca , Merrick Jillian S. , Ramakrishnan Jyothi L. , Thibodeau Eric L. , Masten Ann S. 
Published: January 2023

AbstractParenting shapes the development of emotion regulation skills in early childhood, laying a key foundation for social‐emotional adjustment. Unfortunately, high adversity exposure may disrupt parental emotion socialization practices and children's regulatory development. The current study used variable‐ and person‐centered approaches to evaluate links among parental emotion expressiveness, children's observed emotion regulation, and teacher‐reported adjustment among 214 4‐ to 6‐year‐old children experiencing homelessness, an indicator of high cumulative risk and acute adversity. Structured parent‐child interaction tasks were recorded on site in emergency shelters over the summer and micro‐socially coded for parent and child expressions of anger, positive affect, and internalizing distress. We anticipated that parental modeling of predominantly negative emotion expression would be associated with more child dysregulation during parent‐child interaction and worse adjustment at school, as reported by teachers the following school year. Preliminary analyses indicated that children's observed difficulty downregulating anger was associated robustly with teacher‐reported social‐behavioral problems. Latent profile analysis was used to identify three patterns of parental emotion expression characterized by above‐average expression of positive affect, internalizing distress, and anger. Parents’ likelihood of membership in the elevated anger profile significantly predicted children's observed difficulty down‐regulating anger and higher social‐behavioral problems at school. In addition to ongoing efforts to reduce poverty‐related risk, supporting adaptive anger regulation in parents and young children may be important for enhancing resilience among families experiencing homelessness and similar conditions of high cumulative risk.
Progression of human monkeypox infection despite tecovirimat in an immunocompromised adult
Authors: Dammann Fabian , Raja Mohammed , Camargo Jose F. 
Published: January 2023

AbstractWe report a case of an immunocompromised man with monkeypox who experienced disease progression despite timely initiation of tecovirimat and ultimately required utilization of cidofovir and VIGIV for treatment. In immunocompromised patients, monkeypox might present with a more severe course of disease requiring consideration of alternative treatment strategies
PHYTOCHROME‐INTERACTING FACTORS are involved in starch degradation adjustment via inhibition of the carbon metabolic regulator QUA‐QUINE STARCH in Arabidopsis
Authors: Lee Sang Woo , Choi Dasom , Moon Heewon , Kim Sujeong , Kang Hajeong , Paik Inyup , Huq Enamul , Kim Dong‐Hwan 
Published: January 2023

SUMMARYAs sessile organisms, plants encounter dynamic and challenging environments daily, including abiotic/biotic stresses. The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings. Light, one of the essential environmental signals, exerts a substantial impact on plant metabolism and resource partitioning (i.e., starch). However, it is not fully understood how light signaling affects carbohydrate production and allocation in plant growth and development. An orphan gene unique to Arabidopsis thaliana, named QUA‐QUINE STARCH (QQS) is involved in the metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis. In this study, we show that PHYTOCHROME‐INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs), including PIF4, are required to suppress QQS during the period at dawn, thus preventing overconsumption of starch reserves. QQS expression is significantly de‐repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3, and PIF5) act as negative regulators of QQS expression. In addition, we show that the evening complex, including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night‐time. Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in the expression of QQS. This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during the night and prevent overconsumption of starch before its biosynthesis during the upcoming day.
Natural variations in the PbCPK28 promoter regulate sugar content through interaction with PbTST4 and PbVHA‐A1 in pear
Authors: Li Jiaming , Zhu Rongxiang , Zhang Mingyue , Cao Beibei , Li Xiaolong , Song Bobo , Liu Zhongchi , Wu Jun 
Published: January 2023

SUMMARYSoluble sugars play an important role in plant growth, development and fruit quality. Pear fruits have demonstrated a considerable improvement in sugar quality during their long history of selection. However, little is known about the underlying molecular mechanisms accompanying the changes in fruit sugar content as a result of selection by horticulturists. Here, we identified a calcium‐dependent protein kinase (PbCPK28), which is located on LG15 and is present within a selective sweep region, thus linked to the quantitative trait loci for soluble solids. Association analysis indicates that a single nucleotide polymorphism‐13 variation (SNP13T/C) in the PbCPK28 regulatory region led to fructose content diversity in pear. Elevated expression of PbCPK28 resulted in significantly increased fructose levels in pear fruits. Furthermore, PbCPK28 interacts with and phosphorylates PbTST4, a proton antiporter, thereby coupling the sugar import into the vacuole with proton export. We demonstrated that residues S277 and S314 of PbTST4 are crucial for its function. Additionally, PbCPK28 interacts with and phosphorylates the vacuolar hydrogen proton pump PbVHA‐A1, which could provide proton motive forces for PbTST4. We also found that the T11 and Y120 phosphorylation sites in PbVHA‐A1 are essential for its function. Evolution analysis and yeast‐two‐hybrid results support that the CPK‐TST/CPK‐VHA‐A regulatory network is highly conserved in plants, especially the corresponding phosphorylation sites. Together, our work identifies an agriculturally important natural variation and an important regulatory network, allowing genetic improvement of fruit sugar contents in pears through modulation of PbCPK28 expression and phosphorylation of PbTST4 and PbVHA‐A1.
Partial validation of the Vcheck canine pancreatic lipase assay
Authors: Jakus Paulina , Strage Emma , Abrahamsson Johanna , Hillström Anna 
Published: January 2023

AbstractMeasurement of canine pancreatic lipase immunoreactivity (cPLI) is used for diagnosing pancreatitis in dogs. Because pancreatitis can be a life‐threatening disease with severe complications, an in‐house cPLI test would be valuable to obtain rapid test results. The aim of this study was to evaluate a point‐of‐care cPLI test, Vcheck cPL. Precision, determined according to EP15, and linearity under dilution were determined and judged against preset quality goals. Results from the Vcheck cPL were compared with a previously validated cPLI ELISA, Spec cPL. In a retrospective study, cPLI results from dogs with and without acute pancreatitis, as determined by pancreatic ultrasound examination, were investigated to assess the performance of the assay in a clinical setting. Statistical analysis included the Mann–Whitney test, Chi‐square test, and Passing–Bablok regression analysis with a significance level of 0.05. Precision of the assay was acceptable, with intra‐, inter‐, and total coefficients of variation (CV%) less than 12.1%, 6.4%, and 12.1%, respectively. Results from the linearity study indicated that the method was acceptably linear at lower concentrations but not in the high‐concentration range. The method comparison study revealed that Vcheck generally measured higher concentrations compared with Spec cPL, and that the methods should not be used interchangeably. Dogs with acute pancreatitis had significantly higher cPLI concentrations compared with dogs without pancreatitis (P < 0.01), but there was a marked overlap in cPL concentrations between the two groups.
Lipin1 plays complementary roles in myofibre stability and regeneration in dystrophic muscles
Authors: Jama Abdulrahman , Alshudukhi Abdullah A. , Burke Steve , Dong Lixin , Kamau John Karanja , Voss Andrew Alvin , Ren Hongmei 
Published: January 2023

AbstractDuchenne muscular dystrophy (DMD) is a severe muscle wasting disorder caused by dystrophin mutations, leading to the loss of sarcolemmal integrity, and resulting in progressive myofibre necrosis and impaired muscle function. Our previous studies suggest that lipin1 is important for skeletal muscle regeneration and myofibre integrity. Additionally, we discovered that mRNA expression levels of lipin1 were significantly reduced in skeletal muscle of DMD patients and the mdx mouse model. To understand the role of lipin1 in dystrophic muscle, we generated dystrophin/lipin1 double knockout (DKO) mice, and compared the limb muscle pathology and function of wild‐type B10, muscle‐specific lipin1 deficient (lipin1Myf5cKO), mdx and DKO mice. We found that further knockout of lipin1 in dystrophic muscle exhibited a more severe phenotype characterized by increased necroptosis, fibrosis and exacerbated membrane damage in DKO compared to mdx mice. In barium chloride‐induced muscle injury, both lipin1Myf5cKO and DKO showed prolonged regeneration at day 14 post‐injection, suggesting that lipin1 is critical for muscle regeneration. In situ contractile function assays showed that lipin1 deficiency in dystrophic muscle led to reduced specific force production. Using a cell culture system, we found that lipin1 deficiency led to elevated expression levels of necroptotic markers and medium creatine kinase, which could be a result of sarcolemmal damage. Most importantly, restoration of lipin1 inhibited the elevation of necroptotic markers in differentiated primary lipin1‐deficient myoblasts. Overall, our data suggests that lipin1 plays complementary roles in myofibre stability and muscle function in dystrophic muscles, and overexpression of lipin1 may serve as a potential therapeutic strategy for dystrophic muscles.
imageKey points
We identified that lipin1 mRNA expression levels are significantly reduced in skeletal muscles of Duchenne muscular dystrophy patients and mdx mice.
We found that further depletion of lipin1 in skeletal muscles of mdx mice induces more severe dystrophic phenotypes, including enhanced myofibre sarcolemma damage, muscle necroptosis, inflammation, fibrosis and reduced specific force production.
Lipin1 deficiency leads to elevated expression levels of necroptotic markers, whereas restoration of lipin1 inhibits their expression.
Our results suggest that lipin1 is functionally complementary to dystrophin in muscle membrane integrity and muscle regeneration.
Analyzing Extreme Sea State Conditions by Time-Series Simulation Accounting for Seasonality
Authors: Vanem Erik 
Published: January 2023

AbstractThis article presents an extreme value analysis on data of significant wave height based on time-series simulation. A method to simulate time series with given marginal distribution and preserving the autocorrelation structure in the data is applied to significant wave height data. Then, extreme value analysis is performed by simulating from the fitted time-series model that preserves both the marginal probability distribution and the autocorrelation. In this way, the effect of serial correlation on the extreme values can be taken into account, without subsampling and de-clustering of the data. The effect of serial correlation on estimating extreme wave conditions have previously been highlighted, and failure to account for this effect will typically lead to an overestimation of extreme conditions. This is demonstrated by this study, which compares extreme value estimates from the simulated times-series model with estimates obtained directly from the marginal distribution assuming that 3-h significant wave heights are independent and identically distributed. A dataset of significant wave height provided as part of a second benchmark exercise on environmental extremes that was presented at OMAE 2021 has been analyzed. This article is an extension of a study presented at OMAE 2022 (OMAE2022-78795) and includes additional preprocessing of the data to account for seasonality and new results.
Transient behavior of a salinity gradient solar pond under Mediterranean climate
Authors: Rghif Yassmine , Sayer Asaad , B. Mahood Hameed 
Published: January 2023

Abstract
This paper aims to investigate the transient behavior of a salinity gradient solar pond(SGSP) under Mediterranean climate. For this propose, a 2D numerical model is developed in which the absorption of solar radiation by different layers of saline water, the wind effect at free surface, the heat losses and the development of the double-diffusive convection in the lower and upper convective zones are considered. The governing equations of continuity, momentum, thermal energy and diffusion are solved using the finite volume method with SIMPLE algorithm. The validity of the numerical model developed in Fortran95 is achieved through the comparisons of the results computed with the available numerical and experimental results obtained by literature studies. Results show that the developed numerical model can predict transient behavior of the SGSPs with a good accuracy. As an application of this model, temperature, salt concentration, energy stored and storage efficiency variations of a proposed SGSP are analyzed under Mediterranean climate. The results show that the lower convective zone(LCZ) temperature increases from 15 °C to around 95 °C whereas the temperature of the upper convective zone(UCZ) varies sinusoidally depending on that of the ambient air. Furthermore, the salt concentration of the LCZ decreases from 250kg/m3 to around 248kg/m3 while that of the UCZ increases from50 kg/m3 to about 52kg/m3. Additionally, the thermal energy stored is around 135MJ with an efficiency of about 38% which confirms the capacity of the SGSP to store thermal energy as sensible heat.