bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression Pattern and Prognostic Implication of SALL4 Gene in Acute Myeloid Leukemia
Authors: Shalaby Nahela Ahmed , Khattab Ahmed Mahmoud Taha , Ibrahim Shereen Abd El-Monem 
Published: August 2023

Abstract

Background
Acute myeloid leukemia (AML) is a clonal disorder arises from the differentiation arrest of myeloid precursor and malignant proliferation of a bone marrow derived, self-renewing stem or progenitor cells in the bone marrow (BM) and blood.

Aim of the Work
To characterize the expression pattern of SALL4 gene in patients with acute myeloid leukemia and to determine its prognostic impact.

Patients and Methods
This study was conducted on 20 Egyptian AML patients (recruited from hematology units of Internal Medicine department, Ain shams University Hospitals) and 10 patients as controls. All patients were subjected to full history taking, thorough clinical examination, complete blood count, BM aspiration cytochemical analysis, flow-cytometric immunophenotyping of BM blasts using acute leukemia panel and cytogenetic analysis using FISH technique and RT-PCR to measure SALL4 gene expression in AML cases and controls.

Results
Our results showed highly significant difference between expression of SALL 4 gene in AML patients than control group with RQ median in AML cases 15.46 and in control group 0.4 (p value 0.000). Non significant results about prognosios was reached

Conclusion
SALL4 gene expression is constitutive in De-novo AML cases.
Evaluation of Polymyxin NP Test as a Rapid Method for Detection of Colistin Resistance in Enterobacteriaceae
Authors: El-masry Shereen Ahmed , Fahim Noha Alaa Eldin , Mohammed El-Beih AmiraSaad 
Published: August 2023

Abstract

Background
The carbapenemase-producing Enterobacteriaceae are among the most clinically significant multidrug-resistant bacteria. Its rise has led to an increase in the use of the polymixin B and colistin because of limited effective alternative antibiotic treatment.

Aim of the Work
Evaluation of the diagnostic efficacy of the rapid polymyxin NP test for the detection of colistin-resistant Enterobacteriaceae.

Materials and Methods
The study included a total of 42 Enterobacteriaceae isolates. All isolates were subjected to determination of the minimum inhibitory concentration (MIC) for colistin and polymyxin B using the broth microdilution method and detection of colistin-resistant isolates using the polymyxin NP test.

Results
Compared to the colistin MIC, the polymyxin NP test overall sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 64.7%, 92%, 84.6% and 79.3% respectively. On the other hand, a higher sensitivity (91.6%), specificity (93.3%), PPV (84.6%) and NPV (95.5%) were obtained when the results were compared to the polymyxin B MIC results.

Conclusion
The rapid polymyxin NP test is easy-to-perform, rapid, sensitive, specific test that can be used for the routine detection of polymyxin resistant Enterobacteriaceae particularly in poor resource laboratories.
The Effect of Lactoferrin Supplementation on Iron level and Haematological Parameters after Sleeve Gastrectomy
Authors: Reda Randa , Mohamed Omnia , El Fouly Mohamed , Samy Hassan Asmaa 
Published: August 2023

Abstract

Background
Sleeve gastrectomy (SG) is one of the most effective bariatric surgery methods of treatment for obesity. It can cause nutritional deficiencies and lead to anemia at the same time.

Aim and objectives
To evaluate the effect of lactoferrin supplementation on iron level and haematological parameters after three months of supplementations for sleeve gastrectomy patients.

Subjects and Methods
This study is a randomized control clinical study, Forty patients after sleeve gastrectomy divided into two groups as follows: (Group 1): 20 cases received ferrous sulphate capsule once daily for 3 consecutive months, (Group 2): 20 cases received lactoferrin sachet twice daily for 3 consecutive months with ferrous sulfate capsule once daily.

Result
There is a significant increase in hemoglobin, HCT, and RBCs after 3-months follow up on ferrous sulfate supplementation. Meanwhile, there is a significant decrease in serum ferritin after 3 months follow up. However, the improvement in hematological parameters was more significant in the group of lactoferrin with ferrous sulfate.

Conclusion
Oral lactoferrin was better tolerated and more acceptable in combination with oral iron with higher increase in hematological parameters when compared to oral iron therapy alone over three-months treatment.
The Expression of CD47 and Its Association with Other Biochemical Storage Lesions in Stored Non-Leucoreduced versus Leucoreduced Red Blood Cell Units
Authors: Abdel-Messih Ibrahim Y , Bassiouny Noha , Nader Hebat Allah , Khaled Dalia 
Published: August 2023

Abstract

Background
The emergent need for blood transfusion required blood storage at low temperature which induced biochemical and biomechanical changes, collectively known as RCSL(red cell storage lesion). CD47 is an integrin associated protein which decreases with PRBCs(packed red blood cells) prolonged storage that possibly leads to less effective blood transfusion. Electrolyte imbalance occurs due to inactivation of Na+/K+ ATPase pump in low temperature which inturn leads to continuous increase of extracellular potassium which is considered life threatening for patients with renal failure or those with massive transfusions.

Aim of the Work
The aim of our study is to Evaluate the expression of CD47 by flow cytometry with other biochemical storage changes (pH, sodium and potassium ions) in CPD/SAGM packed non-leucoreduced Group(A) versus CPD/SAGM leucoreduced Group(B) red blood cell units at different storage times (on day 7, 14, 28 from date of red blood cells collection).

Subjects and Methods
450 ml of whole blood is obtained into Quadruple blood bags and further subdivided into 20 non-leucoreduced units and 20 leucoreduced units. Non-leucoreduced and leucoreduced units are stored at 4 ± 2◦C. Samples are taken from each packed red blood cell unit at days 7,14 and 28 and tested for (Na+&K+) ions, pH and CD47 marker by flow cytometry.

Results
Both leucoreduced and non-leucoreduced units show a highly significant decrease in sodium, pH and CD47 expression and a highly significant increase in potassium level. However, there was no statistically significant difference found between 2 studied groups regarding sodium & potassium level, pH and CD47 expression on packed red cells except for sodium on 28th day which was slightly higher in leucoredced than non-leucoreduced units.

Conclusion
Red cell storage lesion is an inevitable process. Our study showed a highly statistically significant decrease in CD47 by flow cytometry in both leucoreduced and non-leucoreduced PRBCs along the storage period from day 7 to day 28. Red cell concentrates show significant decrease in sodium and pH and increase in the level of potassium during storage. Unlike many other studies, leucoreduction didn’t affect the expression of neither CD47 nor biochemical parameters (Na, K and pH) from day 7 to day 28 of PRBc storage.
New Prognostic Indicators in CLL: Relation to Disease Burden
Authors: El Hamid Mohamed Amal Abd , Safwat Nesma Ahmed , Kader Gelan Hosny Abbas Abdel 
Published: August 2023

Abstract

Background
Identifying prognostic markers is important for the clinical and pathological course of chronic lymphocytic leukemia (CLL). Cluster of differentiation CD49d expression and percentage of smudge cells in CLL patients have recently been reported as newer prognostic factor.

Aim of the Study
To estimate CD49d expression as an independent prognostic marker in CLL patients and to investigate its relation to the known prognostic factors

Methods
Thirty adults with CLL were enrolled. Smudge cells percentage was calculated by microscopic evaluation of blood smears. Testing of cluster of differentiation CD49d expression was done by flowcytometry and detection of ataxia telangiectasia mutated (ATM) gene, protein 53(P53) gene deletions and trisomy 12 were performed using fluorescent in situ hybridization (FISH).

Results
CD49d expression was associated with lymphadenopathy, splenomegaly, advanced staging, high total leucocytic count, immunophenotyping scoring, CD38 positivity, short LDT, low smear cell percentage, and presence of p53 deletion. Low percentage of Smear cell (&lt;30%) showed a significantly higher incidence of lymphadenopathy and organomegaly. Additionally, it was associated with a high total leucocytic count, low platelet count, high atypical lymphocytic count and typical IPT scoring, shorter LDT, positive CD38 expression and CD49d expression and ATM and P53 deletions.

Conclusion
CD49d expression and low smudge cell percent were consistently associated with poor prognostic indicators, suggesting that they could be considered as adverse predictors and they should be merged together to improve the power of the prognostic panels of CLL in the clinical setting.
Study of the Effect of ABCB1 Single Nucleotide Polymorphism on Tacrolimus Dose Requirements in Liver Transplant Patients
Authors: El-hadidi Eman Saleh , El-Din Maha Mohsen Kamal , Saab Azza Abd El-rahman , Ali Heba Hassan , Shararah Maram Saeed AbdEl-baki 
Published: August 2023

Abstract

Background
Liver transplantation (LT) is the only effective radical cure for all types of end stage liver diseases. Major advances have been made in the field of liver transplantation due to improvements in surgical techniques and organ conservation as well as optimization of intensive care and immunosuppressive management.

Aim of the Work
The aim of the present work is to assess the influence of ABCB1 gene polymorphism of liver transplant recipients on blood level and dose requirements of oral tacrolimus, in an attempt to help in designing an individualized tacrolimus regimen for Egyptian liver transplant recipient.

Patients and Methods
The study included 25 liver transplant recipients and their respective donors. All subjects of this study were subjected to full medical history, Clinical evaluation, Laboratory investigations, and ABCB1 gene polymorphism evaluation by RT-PCR. Tacrolimus trough level was evaluated for all the recipients during the first 3 months post transplantation.

Results
The present study revealed that the presence of CC genotype was significantly correlated to the effect on tacrolimus C/D ratio and Weight adjusted tacrolimus dose during the first week of the first and 2nd month (Z = -2.108, P &lt; 0.05) but not the 3rd month post transplantation (p-value &gt;0.05). Subjects carrying CC genotype required higher doses of tacrolimus to achieve the desired trough levels compared to subjects carrying CT and TT genotypes. The same effect was observed over the whole period of the study but the results were statistically non-significant (p-value&gt;0.05). Recipients who received liver tissue from donors carrying CC genotype also required higher doses of tacrolimus and reached lower levels of blood tacrolimus trough levels.

Conclusion
The present study revealed that ABCB1 CC genotype of both recipients and donors of liver transplantation was significantly associated with increased required tacrolimus dose early after liver transplantation reaching statistically significant level in the first week of the first and second months.
Association of Glutathione S-Transferase P1 (GSTP1) Gene Polymorphism with the Response to Platinum Based Chemotherapy in Patients with Non-Small Cell Lung Cancer
Authors: Abd El-Mageed Abeer Ibrahim , Mohamed Noha Refaat , Yousry Walaa Ahmed , Nagy Ahmed Aly , Makar Martina Nabil Georges 
Published: August 2023

Abstract

Background
Lung cancer is one of the most commonly diagnosed cancers and despite therapeutic advances, mortality remains high. Platinum based chemotherapy remains the mainstay of adjuvant or first line chemotherapy in non small cell lung cancer (NSCLC) treatment, pathophysiological features along with genetic background significantly influence therapeutic efficacy of platinum-based chemotherapy. GSTP1 gene is a member of the cytosolic superfamily of glutathione-S-transferases, which is responsible for phase II detoxification enzymes that convert various reactive metabolites to more water-soluble and less harmful forms by conjugating them with glutathione. The genetic polymorphism GSTP1 rs1695 is associated with decreased substrate specific catalytic activity and the reduced enzyme activity which may reduces platinum detoxification, thus promoting better response rates to platinum-based chemotherapy.

Aim of the Work
In the present study was aimed to investigate the association of GSTP1 gene polymorphism with the response to platinum based chemotherapy in patients with NSCLC in order to prevent the non necessary exposure to the toxic effect of chemotherapy.

Patients and Methods
The study was a retrospective cross sectional study conducted on 50 NSCLC patients who were recruited from the Oncology clinic in Ain Shams University Hospitals, twenty of them were responders to platinum based chemotherapy and the other 30 were not responders according to RECIST criteria. All subjects underwent full medical history focusing on tobacco smoking and family history of cancers, thorough clinical examination, histological diagnosis of NSCLC and TNM classification, radiological studies: computed tomography of chest and abdomen and laboratory investigations in the form of: complete blood picture (CBC), liver function tests and kidney function tests. Detection of the GSTP1 rs1695 (A/G) polymorphism by real-time PCR was done for both the responders and the non responders group.

Results
There was no statistically significant difference between the responders’ and non responders’ groups regarding the genotypic distribution (p &gt; 0.05). However, the G allele was significantly higher in the responders’ group compared to the non responders’ group with p value &lt; 0.05.

Conclusion
The present study did not confirm the presence of significant association between the genotypic frequencies of the GSTP1 rs 1695 among the responders and the non responders group, but despite that it showed that the A allele was significantly higher in the non responders’ group compared to the responders’ group. However, the G allele was significantly higher in the responders’ group compared to the non responders’ group. Further studies with higher sample size are needed to clarify the association.
Association of Low-Density Lipoprotein Receptor-Related Protein-5 (LRP5) 4037C&gt;T Polymorphism with Nephropathy in Patients with Type 1 Diabetes Mellitus
Authors: Al Hala Abdel , Shaaban Rasha ElAdawy , Awad Doaa Mostafa , El-deen Ibrahim Norhan Hossam 
Published: August 2023

Abstract

Background
Type 1 diabetes mellitus (T1DM) is a heterogeneous and multifactorial endocrinal chronic disease. Genetic predisposition has a major impact on disease susceptibility. Microvascular complications such as diabetic nephropathy develops in T1DM in untreated cases.

Aim of the Study
the study investigated the association between low-density lipoprotein receptor-related protein 5 (LRP5) polymorphism 4037C&gt;T and type 1 diabetes mellitus and the development of diabetic nephropathy.

Patients and Methods
This study included 40 T1DM pediatric patients and 20 age and sex matched healthy controls. All participants of the study were subjected to full history taking, clinical assessment, laboratory investigations and LRP5 gene (rs3736228) polymorphism assay by real time PCR.

Results
The present study showed no statistically significant difference between T1DM patients and their matched healthy subjects as regards LRP5 rs3736228 gene polymorphism. No statistically significant association was observed between LRP5 gene polymorphism and the development of diabetic nephropathy. Patients with CT genotype had a statistically significant association with obesity and hypercholesterolemia.

Conclusion
No association was found between LRP5 gene polymorphism rs3736228 and type 1 diabetes mellitus as well as the development of diabetic nephropathy among the studied pediatric population.
Identification of TIM-3 Expression in Acute Myeloid Leukemia: Clinical and Laboratory Correlation
Authors: Wahba Mona Ahmed Hassan , Safwat Nesma Ahmed , EL-Sayed Shaymaa Ahmed 
Published: August 2023

Abstract

Background
Acute myeloid leukemia (AML) is an aggressive highly heterogenous hematological malignancy associated with poor prognosis and high mortality rate. Both cancer cells and immune cells participate in tumor initiation, growth and progression and might affect clinical outcomes. Indeed, leukemic cells exploit a variety of mechanisms to evade immune response, leading to disease progression and relapse. T-cell immunoglobulin and mucin domain 3 (TIM-3), an immune checkpoint molecule, is expressed not only on immune cells but also on leukemic stem cells (LSCs) in AML. Studies relating to TIM-3 function in AML are limited, and the role of TIM3 in maintaining leukemic stem cells and contributing to the suppression of the anti-tumor immune response is still poorly understood.

Aim of the Work
To investigate TIM-3 expression in patients with AML and explore the impact of its expression on the clinico-laboratory characteristics and prognostic behavior of denovo-AML patients.

Methods
A total of 45 patients newly diagnosed AML patients were tested for TIM-3 expression by flowcytometry at diagnosis.

Results
TIM-3 was found to be over expressed in patients with AML with significant association with the different FAB subtypes (p = 0.010). TIM-3 positivity was negatively correlated with CD34 expression (p = 0.019.). However, no significant association between TIM-3 expression and extramedullary disease (EMD) as well as cytogenetic risk groups.

Conclusion
TIM-3 is overexpressed in AML patients. The prognostic value of TIM3 in AML is not clear and remains to be further explored.
Mean Platelet Volume (MPV) and Plasma Lactate Level in the Diagnosis and Prognosis of Neonatal Bacteremia
Authors: EL-Ashry Marwa Abd EL-Rasoul , Fahim Noha Alaa El-Din Mohammed , Saleh Ghada Ahmed , Hosny Yara Raafat 
Published: August 2023

Abstract

Background
Neonatal sepsis is a life-threatening clinical condition. It is associated with high morbidity and mortality if not treated properly. Blood culture remains the gold standard method diagnosis of sepsis, but it takes at least 24 hours for presumptive diagnosis. Owing to the fact that neonates are vulnerable and can deteriorate easily, rapid diagnosis and management is a must.

Aim of the Work
Determination of the role of mean platelet volume (MPV) and Blood Lactic acid (BLA) level in the diagnosis and prognosis of neonatal bacteremia.

Materials and Methods
Study included 108 clinically septic neonates aged 0-28 days, admitted to NICU in Ain Shams University Hospitals. All neonates were subjected to blood culture as gold standard method, complete blood picture (for MPV evaluation), CRP, and BLA, then they were equally divided into two groups bacteremic group, and non-bacteremic group.

Results
The current study showed that there was a significant difference between bacteremic and nonbacteremic group as regards MPV, CRP, and BLA with higher mean and median values among bacteremic group with P value 0.001, 0.003, and 0.021 respectively. High BLA level was found to be highly significant in non-survived neonates when compared to the survived ones with P value 0.001.

Conclusion
MPV and BLA tests are simple, rapid, and inexpensive methods to diagnose neonatal bacteremia. The available evidence confirms significantly higher MPV, and BLA in neonates with bacteremia compared to neonates with non-bacteremia causes of sepsis. Therefore, in clinical practice, MPV and BLA could be used as indicators for the early diagnosis of sepsis, while blood lactic acid could be used additionally as a predictor of mortality.
Evaluation of Daylight Methylene Blue Photodynamic Therapy in Multiple Recurrent Molluscum Contagiosum
Authors: Beshay Abanoub Samy Abdallah , El Kahky Hanan , Mohammad Ghada Fathy 
Published: August 2023

Abstract

Background
Molluscum contagiosum is a common viral infection caused by DNA Poxvirus. Daylight-photodynamic therapy (DL-PDT) is defined as a photochemical reaction used to selectively destroy tissue.

Objectives
To evaluate the efficacy and safety of daylight methylene blue photodynamic therapy in treatment of multiple recurrent molluscum contagiosum.

Subject and methods
This is a single blinded controlled prospective interventional clinical study including 20 patients with molluscum contagiosum diagnosed clinically and by dermoscopy. Patients subjected to daylight methylene blue-mediated photodynamic therapy. Topical MB liposomal loaded 10% cream was applied over the lesions area with occlusion and left for 15 minutes. Two leisons were left as control in each studied patient. Patients were instructed to stay outdoors exposing their lesions and the adjacent area to daylight continuously for at least 90 minutes (if it was a sunny day) or 120 minutes (if it was an overcast or partially sunny day) between 11:00 am to 02:00 pm. Treatment sessions were done weekly for 10 weeks or till complete clearance. Follow up of the patients for 6 months after end of study to detect recurrence or appearance of new lesions.

Results
Daylight MB Mediated PDT response in a total of 20 (100%) studied patients showed Excellent response to treatment shown as complete clearance in 65%( no.=13) of patients, very good response to treatment in 15.0%( no.=3), good response to treatment in 10.0%( no.=2) and poor response to treatment in 10.0%( no.=2). The total number of lesions was 313. Response of the lesions in studied patents to MB-PDT was monitored by dermoscopic follow up of lesions. Lesions showed complete response in 66.5% (no. = 208) of lesions, partial response in 25.2% (no. = 79) of lesions and no response in 8.3% (no. = 26) of lesions. No serious side effects and also MB-DL/PDT is a painless treatment. No relapse was detected at the site of cleared MC or at a near site during the follow-up period of 6 months following the procedure.

Conclusion
Daylight-PDT using MB is effective in clearing MC lesions, also is safe, easy to carry out, economic, acceptable cosmetic results with no recurrence, convenient especially for children as it is a painless treatment.