bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental DNA phylogeography: Successful reconstruction of phylogeographic patterns of multiple fish species from cups of water
Authors: Tsuji Satsuki , Shibata Naoki , Inui Ryutei , Nakao Ryohei , Akamatsu Yoshihisa , Watanabe Katsutoshi 
Published: March 2023

AbstractPhylogeography is an integrative field of science linking micro‐ and macro‐evolutionary processes, contributing to the inference of vicariance, dispersal, speciation, and other population‐level processes. Phylogeographic surveys usually require considerable effort and time to obtain numerous samples from many geographical sites covering the distribution range of target species; this associated high cost limits their application. Recently, environmental DNA (eDNA) analysis has been useful not only for detecting species but also for assessing genetic diversity; hence, there has been growing interest in its application to phylogeography. As the first step of eDNA‐based phylogeography, we examined (1) data screening procedures suitable for phylogeography and (2) whether the results obtained from eDNA analysis accurately reflect known phylogeographic patterns. For these purposes, we performed quantitative eDNA metabarcoding using group‐specific primer sets in five freshwater fish species belonging to two taxonomic groups from a total of 94 water samples collected from western Japan. As a result, three‐step data screening based on the DNA copy number of each haplotype detected successfully eliminated suspected false positive haplotypes. Furthermore, eDNA analysis could almost perfectly reconstruct the phylogenetic and phylogeographic patterns obtained for all target species with the conventional method. Despite existing limitations and future challenges, eDNA‐based phylogeography can significantly reduce survey time and effort and is applicable for simultaneous analysis of multiple species in single water samples. eDNA‐based phylogeography has the potential to revolutionize phylogeography.
Rates of adherence to cancer treatment guidelines in Australia and the factors associated with adherence: A systematic review
Authors: Bierbaum Mia , Rapport Frances , Arnolda Gaston , Tran Yvonne , Nic Giolla Easpaig Brona , Ludlow Kristiana , Clay‐Williams Robyn , Austin Elizabeth , Laginha Bela , Lo Chi Yhun , Churruca Kate , van Baar Lieke , Hutchinson Karen , Chittajallu Renuka , Owais Syeda Somyyah , Nullwala Ruqaiya , Hibbert Peter , Fajardo Pulido Diana , Braithwaite Jeffrey 
Published: March 2023

AbstractAdherence to cancer treatment clinical practice guidelines (CPGs) varies enormously across Australia, despite being associated with improved patient outcomes. This systematic review aims to characterize adherence rates to active‐cancer treatment CPGs in Australia and related factors to inform future implementation strategies. Five databases were systematically searched, abstracts were screened for eligibility, a full‐text review and critical appraisal of eligible studies performed, and data extracted. A narrative synthesis of factors associated with adherence was conducted, and the median adherence rates within cancer streams calculated. A total of 21,031 abstracts were identified. After duplicates were removed, abstracts screened, and full texts reviewed, 20 studies focused on adherence to active‐cancer treatment CPGs were included. Overall adherence rates ranged from 29% to 100%. Receipt of guideline recommended treatments was higher for patients who were younger (diffuse large B‐cell lymphoma [DLBCL], colorectal, lung, and breast cancer); female (breast and lung cancer), and male (DLBCL and colorectal cancer); never smokers (DLBCL and lung cancer); non‐Indigenous Australians (cervical and lung cancer); with less advanced stage disease (colorectal, lung, and cervical cancer), without comorbidities (DLBCL, colorectal, and lung cancer); with good‐excellent Eastern Cooperative Oncology Group performance status (lung cancer); living in moderately accessible places (colon cancer); and; treated in metropolitan facilities (DLBLC, breast and colon cancer). This review characterized active‐cancer treatment CPG adherence rates and associated factors in Australia. Future targeted CPG implementation strategies should account for these factors, to redress unwarranted variation particularly in vulnerable populations, and improve patient outcomes (Prospero number: CRD42020222962).
Effect of disturbance on bird feeding guilds in a West African dry forest
Authors: Yilangai R. M. , Abalaka J. , Nsor C. A. , Babale A. , Karau S. D. , Ivande S. 
Published: March 2023

AbstractWest African birds have been declining due to intensive deforestation, yet how bird communities respond to such habitat modification remains understudied in this region. We used point transects to survey bird species in the undisturbed and disturbed edges of Tula Mountains Woodland (TMW), in order to examine responses of bird feeding guilds to disturbance. We found significantly more species in the disturbed than in the undisturbed sites. Number of insectivores was significantly higher than other feeding guilds, followed by the granivores, while nectarivores were the fewest. The lack of variation in mean species richness of bird feeding guilds per timed count between habitats indicates that members of all feeding guilds were widespread across the two habitats. However, the higher species numbers of the carnivores and granivores in the disturbed habitat, and the insectivores in the undisturbed habitat suggest their potential affinities for these habitat scenarios. On the other hand, one‐third of the frugivores and nectarivores utilised the two habitat types, indicating generalist behaviour. Our results suggest that disturbance was not as intensive as to cause significant changes in composition of bird feeding guilds at TMW, but increase in disturbance can potentially have adverse effects on the insectivores, as evidenced in their decreased number of species in the disturbed edges.
Iatrogenic duodenal diverticulum perforation: a systematic review
Authors: Boot Madison , Chew Kenneth , Archer Jack , Sowter Steven , Bergamin Paul 
Published: March 2023

AbstractBackgroundDuodenal diverticulum occurs in approximately 20% of the population and can lead to life‐threatening complications such as perforation. Most perforations are secondary to diverticulitis, with iatrogenic causes being exceptionally rare. This systematic review explores the aetiology, prevention and outcomes of iatrogenic perforation of duodenal diverticulum.MethodsA systematic review was performed according to the PRISMA guidelines. Four databases were searched, including Pubmed, Medline, Scopus and Embase. The primary data extracted were clinical findings, type of procedure, prevention and management of perforation and outcomes.ResultsForty‐six studies were identified, of which 14 articles met inclusion criteria and comprised 19 cases of iatrogenic duodenal diverticulum perforation. Four cases identified duodenal diverticulum pre‐intervention, nine were identified peri‐intervention, and the remainder were identified post‐intervention. Perforation secondary to endoscopic retrograde cholangiopancreatography (n = 8) was most common, followed by open and laparoscopic surgery (n = 5), gastroduodenoscopy (n = 4) and other (n = 2). Operative management with diverticulectomy was the most frequent treatment (63%). Iatrogenic perforation was associated with 50% morbidity and 10% mortality.ConclusionIatrogenic perforation of duodenal diverticulum is exceptionally rare and associated with high morbidity and mortality. There are limited guidelines surrounding standard perioperative steps to prevent iatrogenic perforations. A review of preoperative imaging helps identify potential aberrant anatomy, such as a duodenal diverticulum, to allow for recognition and prompt management initiation in the event of perforation. Intraoperative recognition and immediate surgical repair are safe options for this complication.
Shareholders’ Perception of Auditor Type and Timing of Auditor Engagement: Evidence from Auditor Ratification
Authors: Howard Jennifer K. , Son Myungsoo , Song Hakjoon 
Published: March 2023

AbstractMany companies present their selected auditor to shareholders for ratification. In this study, we use auditor ratification voting results to examine whether shareholders view the type of auditor and the timing of the engagement as indicative of audit quality. The auditor ratification literature has yet to explicitly examine these research questions. Our study is unique in that we focus on the auditor switch setting and test how shareholders view a newly hired auditor. We find that shareholders are less likely to approve a non‐Big N auditor than a Big N auditor. We also document that shareholders view late auditor engagements (i.e., during or after the fourth quarter) more negatively, consistent with the argument that late appointment of new auditors should be viewed with caution. The timing effect is particularly acute for non‐Big N firms; however, shareholders do not seem to mind late engagements of Big N auditors.
Anakinra and dexamethasone treatment of idarubicin‐induced mucositis and diarrhoea in rats
Authors: Kullenberg Fredrik , Peters Karsten , Sjöblom Markus , Heindryckx Femke , Dahlgren David , Lennernäs Hans 
Published: March 2023

AbstractChemotherapy‐induced mucositis, characterized by diarrhoea and villous atrophy, is a severe side effect contributing to reduced quality of life and premature death in cancer patients treated with cytostatics. Despite its high incidence, there is no effective supportive therapy available. The main objective of this study was to determine if the anti‐inflammatory drugs anakinra and/or dexamethasone—which have different mechanisms‐of‐action—might be used to effectively treat idarubicin‐induced mucositis in rats. Mucositis was induced through a single injection with 2 mg/kg idarubicin (with saline as control), followed by daily treatments of anakinra (100 mg/kg/day), dexamethasone (10 mg/kg/day) or both for 3 days. After 72 h, jejunal tissue was collected for morphological, apoptotic and proliferative analyses, and colonic faecal water content and body weight change were determined. The diarrhoea that was induced by idarubicin (from 63.5% to 78.6% water content in faeces) was completely reversed by anakinra alone, and the jejunal villus height reduction by 36% was prevented by a combination of anakinra and dexamethasone. Dexamethasone reduced apoptosis in the jejunal crypts, both alone and in combination with anakinra. These positive effects encouraged further investigations into the use of anakinra and dexamethasone as supportive therapies for chemotherapy‐induced intestinal mucositis and diarrhoea.
The effect of transparency on the temporal spillover effect of default nudges
Authors: van Rookhuijzen Merije , de Vet Emely , Adriaanse Marieke A. 
Published: March 2023

AbstractPrevious studies show that the effects of (non‐transparent) nudges can spill over to later similar decisions without nudges. In the current study, we aimed to determine whether such nudge temporal spillover effects are affected by making nudges transparent. The latter is recommended to (partly) mitigate ethical concerns surrounding the use of nudges. In two experiments, we nudged participants to complete a longer version of a survey. Participants were randomly assigned to either a control, a non‐disclosed nudge (using a default to promote taking the longer survey) condition or a disclosed nudge (in which the use of the default nudge was explained) condition. In both Study 1 (N = 1270) and Study 2 (N = 1258) we observed a temporal spillover effect of the disclosed nudge, suggesting that transparency does not negatively affect the temporal spillover effect.
Time trees and clock genes: a systematic review and comparative analysis of contemporary avian migration genetics
Authors: Le Clercq Louis‐Stéphane , Bazzi Gaia , Cecere Jacopo G. , Gianfranceschi Luca , Grobler Johannes Paul , Kotzé Antoinette , Rubolini Diego , Liedvogel Miriam , Dalton Desiré Lee 
Published: March 2023

ABSTRACTTiming is a crucial aspect for survival and reproduction in seasonal environments leading to carefully scheduled annual programs of migration in many species. But what are the exact mechanisms through which birds (class: Aves) can keep track of time, anticipate seasonal changes, and adapt their behaviour? One proposed mechanism regulating annual behaviour is the circadian clock, controlled by a highly conserved set of genes, collectively called ‘clock genes’ which are well established in controlling the daily rhythmicity of physiology and behaviour. Due to diverse migration patterns observed within and among species, in a seemingly endogenously programmed manner, the field of migration genetics has sought and tested several candidate genes within the clock circuitry that may underlie the observed differences in breeding and migration behaviour. Among others, length polymorphisms within genes such asClockandAdcyap1have been hypothesised to play a putative role, although association and fitness studies in various species have yielded mixed results. To contextualise the existing body of data, here we conducted a systematic review of all published studies relating polymorphisms in clock genes to seasonality in a phylogenetically and taxonomically informed manner. This was complemented by a standardised comparative re‐analysis of candidate gene polymorphisms of 76 bird species, of which 58 are migrants and 18 are residents, along with population genetics analyses for 40 species with available allele data. We tested genetic diversity estimates, used Mantel tests for spatial genetic analyses, and evaluated relationships between candidate gene allele length and population averages for geographic range (breeding‐ and non‐breeding latitude), migration distance, timing of migration, taxonomic relationships, and divergence times. Our combined analysis provided evidence (i) of a putative association betweenClockgene variation and autumn migration as well as a putative association betweenAdcyap1gene variation and spring migration in migratory species; (ii) that these candidate genes are not diagnostic markers to distinguish migratory from sedentary birds; and (iii) of correlated variability in both genes with divergence time, potentially reflecting ancestrally inherited genotypes rather than contemporary changes driven by selection. These findings highlight a tentative association between these candidate genes and migration attributes as well as genetic constraints on evolutionary adaptation.
Trans‐generational immune priming is not mediated by the sex of the parent primed: a meta‐analysis of invertebrate data
Authors: Rutkowski Nicola‐Anne J. , McNamara Kathryn B. , Jones Therésa M. , Foo Yong Zhi 
Published: March 2023

ABSTRACTTraditionally, only vertebrates were thought capable of acquired immune responses, such as the ability to transfer immunological experience vertically to their offspring (known as trans‐generational immune priming, TGIP). Increasing evidence challenges this belief and it is now clear that invertebrates also have the ability to exhibit functionally equivalent TGIP. This has led to a surge in papers exploring invertebrate TGIP, with most focusing on the costs, benefits or factors that affect the evolution of this trait. Whilst many studies have found support for the phenomenon, not all studies do, and there is considerable variation in the strength of positive results. To address this, we conducted a meta‐analysis to answer the question: what is the overall effect of TGIP in invertebrates? Then, to understand the specific factors that affect its presence and intensity, we conducted a moderator analysis. Our results corroborate that TGIP occurs in invertebrates (demonstrated by a large, positive effect size). The strength of the positive effect was related to if and how offspring were immune challenged (i.e. whether they were challenged with the same or different insult as their parents or not challenged at all). Interestingly, there was no effect of the ecology or life history of the species or the sex of the parent or the offspring primed, and responses were comparable across different immune elicitors. Our publication bias testing suggests that the literature may suffer from some level of positive‐result bias. However, even after accounting for potential bias, our effect size remains positive. Publication bias testing can be influenced by diversity in the data set, which was considerable in our data, even after moderator analysis. It is therefore conceivable that differences among studies could be caused by other moderators that were unable to be included in our meta‐analysis. Nonetheless, our results suggest that TGIP does occur in invertebrates, whilst providing some potential avenues to examine the factors that account for variation in effect sizes.
Tumor immune microenvironment predicts the pathologic response of neoadjuvant chemoimmunotherapy in non–small‐cell lung cancer
Authors: Han Rui , Zhang Yimin , Wang Tianhu , Xiao Hualiang , Luo Zhilin , Shen Cheng , Li Jianghua , Zhao Chenglong , Li Li , Zhu Mengxiao , Du Haiwei , Tang Huan , Ma Zheng , Wang Yubo , He Yong 
Published: March 2023

AbstractThe clinical outcome of resectable non–small‐cell lung cancer (NSCLC) patients receiving neoadjuvant chemoimmunotherapy is good but varies greatly. In addition, the pathological response after neoadjuvant chemoimmunotherapy is significantly associated with survival outcomes. The aim of this retrospective study was to identify which population of patients with locally advanced and oligometastatic NSCLC has a favorable pathological response after neoadjuvant chemoimmunotherapy. NSCLC patients treated with neoadjuvant chemoimmunotherapy were enrolled between February 2018 and April 2022. Data on clinicopathological features were collected and evaluated. Multiplex immunofluorescence was performed on pre‐treatment puncture specimens and surgically resected specimens. In total, 29 patients with stages III and IV locally advanced or oligometastatic NSCLC who received neoadjuvant chemoimmunotherapy and R0 resection were enrolled. The results showed that 55% (16/29) of patients had a major pathological response (MPR) and 41% (12/29) of patients had a complete pathological response (pCR). In the stroma area of the pre‐treatment specimen, the higher infiltration of CD3+PD‐L1+ tumor‐infiltrating lymphocytes (TILs) and the lower infiltration of CD4+ and CD4+FOXP3+ TILs were more likely to appear in patients with pCR. However, in the tumor area, the higher infiltration of CD8+ TILs was more likely to appear in patients with non‐MPR. In the post‐treatment specimen, we found increased infiltration of CD3+CD8+, CD8+GZMB+, and CD8+CD69+ TILs and decreased infiltration of PD‐1+ TILs both in the stroma and tumor areas. Neoadjuvant chemoimmunotherapy achieved an MPR rate of 55% and induced greater immune infiltration. In addition, we observed that the baseline TILs and their spatial distribution correlate to the pathological response.
MicroRNA‐324‐3p inhibits osteosarcoma progression by suppressing PGAM1‐mediated aerobic glycolysis
Authors: Weng Yiping , Duan Weihao , Yu Xuecheng , Wu Furen , Yang Daibin , Jiang Yuqing , Wu Jingbin , Wang Muyi , Wang Xin , Shen Yifei , Zhang Yunkun , Xu Hua 
Published: March 2023

AbstractOsteosarcoma (OS) is the most common primary malignant neoplasm of the bone. Recent studies have indicated that the inhibitory effects of microRNA (miR)‐324‐3p could affect the development of numerous cancers. However, its biological roles and underlying mechanisms in OS progression remain unexplored. In this study, miR‐324‐3p expression was markedly reduced in OS cell lines and tissues. Functionally, miR‐324‐3p overexpression suppressed OS progression and was involved in the Warburg effect. Mechanistically, miR‐324‐3p negatively regulated phosphoglycerate mutase 1 (PGAM1) expression by targeting its 3′‐UTR. Moreover, high expression of PGAM1 promoted OS progression and aerobic glycolysis, which were associated with inferior overall survival in patients with OS. Notably, the tumor suppressor functions of miR‐324‐3p were partially recovered by PGAM1 overexpression. In summary, the miR‐324‐3p/PGAM1 axis plays an important role in regulating OS progression by controlling the Warburg effect. Our results provide mechanistic insights into the function of miR‐324‐3p in glucose metabolism and subsequently on the progression of OS. Targeting the miR‐324‐3p/PGAM1 axis could be a promising molecular strategy for the treatment of OS.
Piezo1‐mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor‐β1
Authors: Cai Guanhui , Lu Yahui , Zhong Weijie , Wang Ting , Li Yingyi , Ruan Xiaolei , Chen Hongyu , Sun Lian , Guan Zhaolan , Li Gen , Zhang Hengwei , Sun Wen , Chen Minglong , Zhang Wei‐Bing , Wang Hua 
Published: March 2023

AbstractMacrophages are multifunctional immune system cells that are essential for the mechanical stimulation‐induced control of metabolism. Piezo1 is a non‐selective calcium channel expressed in multifarious tissues to convey mechanical signals. Here, a cellular model of tension was used to study the effect of mechanical stretch on the phenotypic transformation of macrophages and its mechanism. An indirect co‐culture system was used to explore the effect of macrophage activation on bone marrow mesenchymal stem cells (BMSCs), and a treadmill running model was used to validate the mechanism in vivo for in vitro studies. p53 was acetylated and deacetylated by macrophages as a result of mechanical strain being detected by Piezo1. This process is able to polarize macrophages towards M2 and secretes transforming growth factor‐beta (TGF‐β1), which subsequently stimulates BMSCs migration, proliferation and osteogenic differentiation. Knockdown of Piezo1 inhibits the conversion of macrophages to the reparative phenotype, thereby affecting bone remodelling. Blockade of TGF‐β I, II receptors and Piezo1 significantly reduced exercise‐increased bone mass in mice. In conclusion, we showed that mechanical tension causes calcium influx, p53 deacetylation, macrophage polarization towards M2 and TGF‐β1 release through Piezo1. These events support BMSC osteogenesis.
Safety and efficacy of everolimus initiation from the first month after liver transplantation: A systematic review and meta‐analysis
Authors: Cholongitas Evangelos , Burra Patrizia , Vourli Georgia , Papatheodoridis George V. 
Published: March 2023

AbstractIntroductionEverolimus, a selective inhibitor of mamalian target of rapamycin (mTORi), is considered to be an alternative immunosuppressive regimen in the liver transplantation (LT) setting. However, most of the transplant centers avoid its early use (i.e., during the first month) after LT mainly due to safety issues.MethodsWe searched for all articles published between 01/2010 and 7/2022 to evaluate the effectiveness and safety of initial/early administration of everolimus after LT.ResultsSeven studies (three randomized controlled trials and four prospective cohort studies) were included: initial/early everolimus‐including therapy (group 1) was used in 512 (51%) and calcineurin inhibitor (CNI) based therapy (group 2) in 494 (49%) patients. No significant difference was found between group 1 and group 2 patients regarding the rates of biopsy‐proven acute rejection episodes (Odds Ratio [OR]: 1.27, 95% CI: .67‐2.41, p = .465) and hepatic artery thrombosis (OR: .43, 95% CI: .09‐2.02, p = .289). Everolimus was associated with higher rates of dyslipidemia (14.2% vs. 6.8%, p = .005) and incisional hernia (29.2% vs. 10.1%, p < .001). Finally, no difference was found between the two groups regarding recurrence of hepatocellular carcinoma (Risk Rates [RR]: 1.22 95%CI: .66‐2.29, p = .524) and mortality (RR: .85 95%CI: .48‐1.50, p = .570).ConclusionUse of initial/early everolimus seems to be effective with a satisfactory safety profile, making its administration a reasonable therapeutic option in the LT setting.