bluuebunny/crossref_metadata_2025_split huggingface dataset:
YTHDC1 regulates the postnatal development of heart
Authors: Shi Lei , Wang Quanwei , Zhang Meiwei , Qin Cong , Shao Dongpu , Peng Mengling , Yang Ying , Shi Wei , Zhang Zhiguo 
Published: September 2023

Abstract
This study aimed to investigate the role of the N6-methyladenosine (m6A) reader protein YTHDC1 in heart development and its potential molecular mechanisms. Animal experiments were conducted using cardiac-specific Ythdc1 knockout (Ythdc1-CKO) mice, and human heart samples were collected from aborted fetuses. Echocardiography, immunoblotting, RNA-Seq, and ATAC-Seq were performed to assess cardiac function, gene expression, and chromatin accessibility. The results revealed that YTHDC1 expression was highest during embryonic and early postnatal stages and gradually decreased with age. Cardiac-specific deletion of Ythdc1 resulted in abnormal heart development, early dilated cardiomyopathy, and severe heart failure. RNA-Seq analysis revealed significant changes in gene expression profiles, particularly genes related to cardiac contraction and transmembrane transport. ATAC-Seq analysis demonstrated significant changes in chromatin accessibility, and the binding motifs of the transcription factors Mef2a, Mef2b, Mef2c, and Mef2d, which are essential for cardiac development, were switched off in Ythdc1-CKO mouse hearts. In conclusion, this study demonstrates that YTHDC1 plays a critical role in heart development and its deficiency leads to abnormal cardiac development and function. The findings provide insights into the molecular mechanisms underlying heart development and suggest potential therapeutic targets for heart diseases.
Neointimal dissection – a rare complication to endovascular treatment in grafts and stent grafts
Authors: Larsen Anne Sofie F. , Aslam Shakil , Holmen Lars Olaf 
Published: September 2023

Abstract
Background.
Neointima formation and hyperplasia in vascular grafts may lead to graft complications threatening the patency of the vascular reconstruction. A rare complication to endovascular treatment of grafts and stent grafts is dissection inside the graft.
Case report.
We present here a case of a 69-year-old female with acute occlusion of the limb of an aorto-bifemoral graft for the third time, 16 years after the primary operation. As at the first two occasions, catheter-based intra-arterial thrombolysis was performed, but with residual stenosis inside the graft. During stent placement, dissection of the neointima or fibrin sheet occluded the inflow to the stent. The complication was resolved with placement of kissing stents.
Conclusions.
It is important to recognize iatrogenic neointima dissection inside graft and stent grafts, as continued thrombolysis will not solve this, but increase the risk of hemorrhagic complications.
CSF1R inhibition is not specific to innate immune cells but also affects T-helper cell differentiation independently of microglia depletion
Authors: Lei Fengyang , Cui Naiwen , Zhou Chengxin , Chodosh James , Vavvas Demetrios G. , Paschalis Eleftherios I. 
Published: September 2023

Abstract
Colony-stimulating factor 1 receptor (CSF1R) inhibition has been proposed as a specific method for microglia depletion. However, recent work revealed that in addition to microglia, CSF1R inhibition also affects other innate immune cells, such as peripheral monocytes and tissue-resident macrophages of the lung, liver, spleen, and peritoneum. Here, we show that this effect is not restricted to innate immune cells only but extends to the adaptive immune compartment. CSF1R inhibition alters the transcriptional profile of bone marrow cells that control T helper cell activation. In vivo or ex vivo inhibition of CSF1R profoundly changes the transcriptional profile of CD4+ cells and suppresses Th1 and Th2 differentiation in directionally stimulated and unstimulated cells and independently of microglia depletion. Given that T cells also contribute in CNS pathology, these effects may have practical implications in the interpretation of relevant experimental data.
Functional analysis of polyphosphate in Myxococcus xanthus
Authors: Harita Daiki , Matsukawa Hiroka , Kimura Yoshio 
Published: September 2023

Abstract
Myxococcus xanthus synthesizes polyphosphates (polyPs) with polyphosphate kinase 1 (Ppk1) and degrades short- and long-chain polyPs with the exopolyphosphatases, Ppx1 and Ppx2, respectively. M. xanthus also expresses polyP:AMP phosphotransferase (Pap) which generates ADP from polyPs and AMP. M. xanthus synthesized polyPs during the stationary phase; the ppk1 mutant showed no difference in growth during the exponential growth phase but died earlier than the wild-type and ppx2 mutant after the stationary phase. In addition, M. xanthus cells cultured in phosphate-starved medium, 0.07 mM H2O2-supplemented medium, or nutrient-deficient medium (CF medium) increased the intracellular polyP levels by six- to eight-fold after 6 h of incubation. However, the growth of ppk1 and ppx2 mutants in phosphate-starved medium and 0.07 mM H2O2 supplemented medium was not significantly different from that of wild-type strains, nor was there a significant difference in fruiting body formation and sporulation on CF medium. The adenylate energy charge (AEC) values of the wild-type strain and the ppk1, ppx2, and pap mutant strains at the exponential growth phase were approximately 0.80. The AEC of the wild-type strain did not change until day 3 of development, whereas the AEC values of the ppk1 and pap mutant strains decreased to 0.77 due to an increase in AMP and a decrease in ADP. Spores of ppk1 and pap mutants in the nutrient medium germinated later than those of the wild-type strain. These results suggested that polyPs produced during development may play an important role in cellular energy homeostasis by being used to convert AMP to ADP via Pap.
Risk factors analysis and prediction model building of infection in patients with autoimmune rheumatic disease
Authors: Li Jinzhou , Zou Danfeng , Luo Xiaoning , Hu Hongling , Zheng Ping , Zhao Jinjun , Mo LiQian 
Published: September 2023

Abstract

Background
Patients with autoimmune rheumatic diseases (AIRDs) are at increased risk of infection, and accurate assessment of infection risk can provide information for clinical decision making. This study is to identify the risk factors associated with infection in patients with AIRDs and develop a risk prediction model.
Methods
The clinical data of AIRDs inpatients was collected and retrospectively analyzed from January 2020 to December 2022. Univariate and multivariate Logistic regression analyses were employed to determine the independent risk factors of comorbid infection in AIRDs patients. A clinical prediction model was constructed and subsequently evaluated using the receiver operating characteristic (ROC) curve.
Results
A total of 281 cases of infection were observed in patients with AIRDs, with a positive sputum culture rate of 36.0%. Among these cases, 128 strains of pathogens were identified, including 72 strains of bacteria and 56 strains of fungi. Additionally, parasite eggs were detected in the stool samples of 2 patients. IgG and glucocorticoid therapy were independent factors influencing the occurrence of infection in patients with AIRDs. The prediction model incorporating IgG demonstrated an area under the receiver operating characteristic curve of 0.751 (95% CI: 0.552–0.951). IgG (≤ 12g/L) can serve as a valuable tool for evaluating the susceptibility to infection in AIRDs patients.
Conclusion
IgG reduction(≤ 12g/L) can serve as a predictive indicator for infection in AIRDs patients, which can assist clinical decision-making by proposing preventive strategies early to reduce infections.
Thermodynamic studу of ethyl alcohol sorption on activated petroleum coke
Authors: Tsareva Anna A. , Poltoratckaya Maria E. , Litvinova Tatiana E. , Rudko Viacheslav A. 
Published: September 2023

Abstract
The low-quality petcoke do not find qualified application and is stockpiled at refineries or used as solid fuel. One of the promising uses of low-quality petroleum coke is its physical or chemical activation in order to obtain a highly porous carbon material that can be used as a catalyst carrier, adsorbent, the basis for capacitive electrodes etc. In this work the possibility of using petroleum coke to produce sorbent for organic compounds was studied. The activated petroleum cake was obtained by chemical activation with KOH, and had a specific surface area of 1218 m2/g. Sorption of ethyl alcohol was studied at temperatures 285, 293, and 300 K. It is a physical process, proceeds mainly in pores of activated petroleum coke. Sorption can be described as a reversible exothermic process. The effective Gibbs energy at a temperature of 293 K is -2.75 kJ/mol. The heat of sorption is -25.65 kJ/mol. The obtained data confirm that porous carbon material obtained from petroleum coke can be used as sorbent for ethanol at room temperature. For example, for adsorption of bioethanol from the effluent of the fermentation process or for purification of wastewater from organic compounds.
Disitamab Vedotin, a HER2-directed Antibody Drug-Conjugate, in Patients with HER2- positive and HER2-low Advanced Breast Cancer：A Phase 1/1b Study
Authors: Wang Jiayu , Liu Yunjiang , Zhang Qingyuan , Li Wei , Feng Jifeng , Wang Xiaoxia , Fang Jianmin , Han Yiqun , Xu Binghe 
Published: September 2023

Abstract
Purpose
Disitamab vedotin (DV; RC48-ADC) is an antibody-drug conjugate comprising HER2-directed antibody, linker and monomethyl auristatin E. This phase 1/1b study evaluated the safety and efficacy of DV in HER2-positive and HER2-low advanced breast cancer (ABC).
Patients and Methods:
In the phase 1 dose-escalation study (C001 CANCER), HER2-positive ABC patients received DV at doses of 0.5–2.5 mg/kg once every two weeks (Q2W) until unacceptable toxicity or progressive disease. The recommended phase 2 dose (RP2D), safety, and pharmacokinetics (PK) were determined. The phase 1b dose-ranging and expansion study (C003 CANCER) enrolled HER2-positive and HER2-low ABC patients receiving DV at doses of 1.5–2.5 mg/kg Q2W and 2.0 mg/kg Q2W, respectively.
Results
24 patients in C001 CANCER and 112 patients in C003 CANCER, including 70 patients with HER2-positive ABC and 66 patients with HER2-low ABC, were enrolled. At the PR2D of 2.0 mg/kg Q2W, confirmed objective response rate (ORR) was 42.9% (9/21; 95% CI 21.8–66.0) and 33.3% (22/66; 95% CI 22.2–46.0), with median progression-free survival (PFS) of 5.7 months (95% CI 5.3–8.4) and 5.1 months (95% CI 4.1–6.6) for HER2-positive and HER2-low ABC, respectively. Common (≥ 5%) grade 3 or higher treatment-emergent adverse events included neutrophil count decreased (17.6%), gamma-glutamyl transferase increased (13.2%), asthenia (11.0%), white blood cell count decreased (9.6%), peripheral neuropathy (6.6%), and pain (5.9%).
Conclusions
DV demonstrated promising efficacy in HER2-positive and HER2-low ABC, with a favorable safety profile at 2.0 mg/kg Q2W.
Trail registration:
NCT02881138. Registered 20 August 2016; NCT03052634. Registered 09 February 2017;
LncRNA FLVCR1-AS1 functions as competing endogenous RNA (ceRNA) to sponge miR-381-3p and aggravate colorectal cancer via upregulation of Wnt signaling pathway
Authors: Beni faeze ahmadi , Abdolvand Mohammad , Salehi Mansoor , Dehghanian Fariba , Kazemi Mohammad 
Published: September 2023

Abstract
Introduction
Colorectal cancer (CRC) is the third most frequent cancer and the second deadliest cancer, worldwide. Long non-coding RNAs (lncRNAs) have been introduced as vital regulators of CRC. lncRNA feline leukemia virus subgroup C receptor 1 antisense RNA 1 (FLVCR1‑AS1) is suggested to play a significant role in the tumorigenesis of several cancers. Wnt signaling pathway is the most deregulated pathway in CRC. The present study aimed to investigate the underlying mechanism of function of FLVCR1-AS1 in CRC through FLVCR1-AS1/miR-381-3p/ CTNNB1, LRP6, FZD3 axis.
Methods
The expression level of FLVCR1-AS1 was compared between CRC tissues and adjacent normal tissues, and additionally between CRC cell lines. Knockdown of FLVCR1-AS1 was performed in HCT116 cells, afterwards, the effects of this knockdown on the expression levels of FLVCR1-AS1, miR-381-3p, and three genes was examined via Real time-PCR. The differences in proliferation were evaluated using MTT assay, and cell death was assessed by flow cytometry.
Results
The results confirmed that FLVCR1-AS1 was upregulated in CRC tissues compared to adjacent normal tissues. RT-qPCR validated that FLVCR1-AS1 has the most level of expression in HT29, HCT116, SW480, and Caco2; respectively. Knockdown of FLVCR1‑AS1 was significantly followed by attenuated viability of HCT116 cells; while resulted in enhanced apoptosis and necrosis.
Conclusion
These findings support the idea that FLVCR1-AS1 may act as an oncogene in CRC and targeting FLVCR1-AS1/miR-381-3p/ CTNNB1, LRP6, FZD3 axis may be introduced as a novel target for CRC therapy and diagnosis in the future.
Dual-energy computed tomography is a superior diagnostic performance for locating parathyroid tumors
Authors: Wang Qian , Yang Wenjing , Wang Jiacheng , Zhao Teng , Feng Dalin , Liu Xing , Shen Hong , Wei Bojun 
Published: September 2023

Abstract
Purpose
To analyze the accuracy of three imaging methods for locating parathyroid tumors preoperatively in patients with primary hyperthyroidism (PHPT).
Methods
We retrospectively analyzed the accuracy of dual-energy computed tomography (DECT), cervical ultrasound (US), and 99m-technetium-labeled methoxyisobutylisonitrile single-photon emission computed tomography/computed tomography (99mTc-MIBI SPECT/CT) for the preoperative localization of abnormal parathyroid glands in 277 patients, who underwent parathyroidectomy from February 2017 to March 2023 in our department. We compared the diagnostic efficiency of the three imaging methods for localization of abnormal parathyroid glands, including those with single parathyroid adenomas or carcinomas, double adenomas (DAs), multiple gland hyperplasia disease (MGHD), and ectopic parathyroid tumors.
Results
A total of 283 pathological parathyroid glands were found in the 277 PHPT patients. The DECT sensitivity of 93.3% was superior to that of US at 92.2% and 99mTc-MIBI SPECT/CT at 75.6%. Ectopic locations were diagnosed by DECT in 12/13 (92.3%) patients by 99mTc-MIBI SPECT/CT in 11/13 (84.6%) patients and by US in 8/13 (61.5%) patients. The diagnostic efficiency was 98.59% for the combination of US and DECT, 97.17% for MIBI and DECT, and 95.76% for US and 99mTc-MIBI SPECT/CT. The diagnostic efficiency of the combination of all three methods for identifying pathological parathyroid glands was 99.29%.
Conclusion
DECT has a higher diagnostic accuracy rate than US and 99mTc-MIBI SPECT/CT for the preoperative localization of abnormal parathyroid glands, and it has advantages for the localization of ectopic parathyroid glands. DECT shows promise as a first-line preoperative localization imaging method for PHPT patients and may lead to significantly improved preoperative localization of pathological parathyroid glands when combined with US and 99mTc-MIBI SPECT/CT.
Genetic Variability of SLCO1B3 and Its Influence on Lipid Levels and Statin Efficacy in Han and Uighur Populations
Authors: Luo Sifu , Jin Menglong , Jureti Subinuer , Ji Hongyu , Liu Ziyang , Maimaitituersun Gulinigaer , Meng Fanhua , Liu Seng , Wei Mengwei , Cao Zichen , Fu Zhenyan 
Published: September 2023

Abstract
Background
Lowering plasma LDL cholesterol levels reduces the risk of atherosclerotic cardiovascular disease (ASCVD). Genetic factors, including single nucleotide polymorphisms (SNPs), affect lipid abnormalities. Statins are the main treatment for lipid disorders, but their effectiveness is influenced by genetics.
Objective
This study examines the impact of SLCO1B3 genetic variability on lipid levels and statin efficacy, particularly on statin sensitivity.
Methods
780 coronary atherosclerotic heart disease patients on oral statins for ≥ 4 weeks were studied. Clinical data and SNP genotyping were collected.
Results
Statin therapy effectively reduces TC, TG, LDL-C, APOA1, and APOB in Han and Uighur populations, with limited impact on HDL-C and Lpa. In the Han population, rs2117032 significantly affects APOB levels, post-statin APOB, Lpa, AST levels, and rate of LDL-C reduction. In Uighurs, rs2117032 significantly affects Tbil levels. In Han individuals, rs3764006 significantly influences TBil, ALT levels, post-statin LDL-C, APOB, Lpa, AST levels, and rates of LDL-C and APOB reduction, correlating with statin sensitivity. In Uighurs, rs3764006 notably affects APOA1 levels. For Han population, rs4149117 significantly impacts LDL-C, TC, TG levels, post-statin TG, ALT, AST levels, and LDL-C reduction. In Uighurs, rs4149117 affects post-statin AST and correlates with statin sensitivity. Additionally, rs2417940 significantly affects Tbil, post-statin TG, and rate of LDL-C reduction in Han, and such effects in Uighurs. In Han population, H3 (C-G-G) haplotype frequencies are higher in statin-sensitive individuals (OR = 1.861, 95% CI: 1.178–2.939, p = 0.007). In Uighurs, H6 (T-A-T) haplotype frequencies are higher in statin-sensitive subjects (OR = 4.906, 95% CI: 1.549–15.541, p = 0.0029).
Conclusion
The genetic polymorphism of SLCO1B3 significantly impacts blood lipid levels, influences liver function to some extent, concurrently exerts a notable influence on the efficiency of statin-induced LDL-C reduction, and is also associated with statin drug sensitivity.
Caregiving Experiences with Health Information Management and Communication in Complex Home Care: Informing Technology Design for Caregivers of Older Adults
Authors: Tennant Ryan , Allana Sana , Mercer Kate , Burns Catherine M. 
Published: September 2023

Abstract
Purpose: The objective of this study was to understand how caregivers manage and communicate health information for older adults who require complex home care, informing the design of new technologies to support patient safety in the home.
Methods: The research involved semi-structured interviews with 15 caregivers, including family and hired caregivers, in Ontario, Canada. An inductive analysis was used to develop themes.
Results: The findings described how participants were Updating the Caregiver Team to share health information in the home. Participants were also Learning to Improve Care & Decision-Making. However, sometimes participants experienced Conflicts within Caregiver Teams using current technologies, which may not fully meet their information management and communication needs.
Conclusion: This research highlights the difficulties of caring for older adults in complex home care situations and the challenges that family and hired caregivers face when managing health information and communication. Currently, paper-based technologies are used, but there is a growing interest in digital tools that can efficiently gather and transform health information to better support decision-making. Collaborative digital systems involving family caregivers as important care team members could improve information sharing and reduce conflicts. However, implementing new technologies in this context can be difficult, and successful adoption may require systems that improve the overall caregiving experience in complex environments. This study recommends integrating caregivers as collaborators and implementing two-way communication in digital systems to enhance caregiver satisfaction. Future research should delve deeper into these complexities and prioritize designing effective tools for this crucial caregiving domain.
The Involvement of the NLRP3 Inflammasome and Pyroptosis in Methamphetamine-Induced Neurotoxicity: Effects on Hippocampal Structure and Memory
Authors: Kurawa Musa Ibrahim , Torkaman-Boutorabi Anahita , Hassanzadeh Gholamreza , Zahmatkesh Maryam , Vousooghi Nasim , Zarrindast Mohammad-Reza , Gholaminejhad Morteza 
Published: September 2023

Abstract
It is known that addictive doses of methamphetamine are neurotoxic; However, it remains unclear whether chronic administration of a lower dose (5 mg/kg) of methamphetamine causes similar neurotoxic effects. This study aimed to assess the effects of chronically administering 5 mg/kg of methamphetamine daily for 1, 2, and 3 weeks on spatial memory, object recognition, passive avoidance memory, hippocampal morphology, and neuroinflammation. Hippocampal morphology was assessed using Nissl stain. The expressions of NLRP3, ASC, and caspase-1 genes were measured as markers of neuroinflammation. Chronic administration of 5 mg/kg of methamphetamine led to significant activation of the inflammasomes (NLRP3, ASC, and caspase-1), resulting in pyramidal cell death in the hippocampus. It also caused impaired spatial memory and passive avoidance learning while leaving recognition memory unaffected. The observed pyramidal cell death is likely due to pyroptosis rather than apoptosis. In conclusion, prolonged administration of 5 mg/kg of methamphetamine was associated with severe inflammasome activation, pyramidal cell death, and mild cognitive decline. Contrary to previous beliefs, even lower doses of methamphetamine taken for an extended period can be neurotoxic.
Language Identity Formation of Second Language Graduate Students in the United States
Authors: Ebrahimi Marzieh 
Published: September 2023

Abstract
Taking social identity theory approach, in this qualitative phenomenological study, researchers investigated how international graduate students from three Asian nations in the United States establish their language identities. The study employs informal interviews with six individuals, from three Asian nations, to inquire about the significance of their individual experiences as they relate to the formation of their linguistic identities. Semi-structured interviews were used to obtain this data since they allowed for an in-depth examination of the participants' experiences. Interviews were analyzed using inductive, thematic data analysis to determine overarching themes. Initial results show that learning a new language presents substantial obstacles for students studying abroad. These include difficulties with pronunciation, communication, and adjustment to culture. Taking language classes and making friends with local students prove to be significant identity-forming experiences. Identity and cultural adaptation are also shown to be influenced by American institutions, such as universities and their professors. The findings of this study can have important significance for institutions which may utilize them to improve the services they offer to international students and create a more welcoming and supportive atmosphere. The study acknowledges its own limitations, including a relatively small sample size and a narrow emphasis on language identity, indicating that more research is needed to investigate the role of language in the formation of international students' identities.