bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation and characterization of core@shell structures of Glycerin@Fe3O4, and Al2O3@Fe3O4 nanoparticles for use in hyperthermia therapy
Authors: Samakosh Saleh Fallah , Bahari Ali , Hamidabadi Vaheed Fallah , Moghaddam Hossain Milani 
Published: April 2023

Abstract
Fe3O4 magnetic nanoparticles (MNPs) have received a great deal of attention in biomedical applications due to their high magnetic sensitivity, large surface area, and superparamagnetic behavior. Their low stability, toxicity, and severe accumulation tendency in aqueous media have, however, hindered their application in hyperthermia treatments. This problem can be resolved by the incorporation of non-magnetic and biocompatible shells on Fe3O4. The aim of this research is thus the introduction of Glycerin and Aluminum oxide (Al2O3) as two proper materials for coating Fe3O4 and the investigation of their efficiency in this field. The results indicated that Glycerin@Fe3O4 and Al2O3@Fe3O4 have a core@shell structure and superparamagnetic features with relatively high saturation magnetization (MS). The cytotoxicity of the synthesized nanoparticles was evaluated on normal fibroblast and lung cancer cell lines (A54912) for 24 and 48 hours using the MTT assay. The viability of the A54912 and fibroblast cell lines in the presence of Fe3O4 was significantly lower than the two coated nanoparticles. This implies that the Glycerin and Al2O3 provided the Fe3O4 nanoparticles with proper biocompatibility. The effectiveness of the synthesized NPs in magnetic hyperthermia was evaluated by exposure of the nanoparticle solutions in water (0.2 and 0.4 mg/ml) to a high-frequency alternating magnetic field for 30 min. the results indicated the achievement of treatment temperature (42–46 ℃) in a short time and elimination of a large percentage of the A54912 cells. In this regard, the present research reported the promising efficacy and efficiency of Glycerin@Fe3O4 and Al2O3@Fe3O4 in hyperthermia-based cancer treatment.
The Effect of Inactivated Vaccine on the Expression of Some Immune-Related Genes in Semislippery Tongue Sole (Cynoglossus Semilaevis)
Authors: yao qiang , shao peng , Zhou Jinyi , Yang Zhanglei , Ji Linting , Yan Wen , Cheng Haosen , Luo Na , Meng Fang 
Published: April 2023

Abstract
In this paper, the whole bacteria inactivated vaccine was prepared by the mermaid subspecies of Mermaid Luminescent Bacillus. The semislippery tongue sole was immunized by immersion and intraperitoneal injection respectively. The liver, kidney, gill and spleen were dissected and RNA was extracted at nine time points, 0h, 4h, 8h, 12h, 24h, 48h, 72h, 96h and 7d after immunization. Detection was carried out in various tissues, using real-time fluorescence quantitative PCR, upon ,the expression changes of the following seven immune-related genes: Toll-like receptor (TLR2), myeloid differentiation factor MyD88, nuclear transcription factor (NF)-κB, co-stimulatory molecule CD28 expressed on the surface of T lymphocytes, CD40, a member of the tumor necrosis factor receptor superfamily, interleukin (IL)-1β, and tumor necrosis factor (TNF) α. The results show：Among the two immunization methods, except for the expression amount of genes of IL-1β in liver and kidney and that of MyD88 in liver in the immersion group being lower than that in the control group, the expression of genes in other tissues showed an upward trend compared with that in control group. In addition, under the immersion immunization mode, except for the TNF-α gene, the tissues with the highest gene expression among the four tissues were gill or spleen；Under injection immunization, the tissues with the highest gene expression among the four tissues were liver or kidney. Also in the immersion group the vast majority of genes showed, at 0h of immune treatment, significantly higher expression than in the control group. The results show that：Both immunization modalities elicited an immunoprotective response in semislippery tongue sole, and there was organizational variability in the expression of immune-related genes through the different immunization modalities.
KLF5 promotes esophageal squamous cell cancer through the transcriptional activation of FGFBP1
Authors: wang fengyun , Luo Ming , Cheng Yufeng 
Published: April 2023

Abstract
Krüpple-like factor 5 (KLF5) is a zinc-finger-containing transcription factor implicated in several human malignancies, but its potential regulatory mechanisms implicated in esophageal squamous cell carcinoma (ESCC) remain elusive. Here, we show that KLF5 is up-regulated in ESCC, where its level was significantly associated with tumor differentiation and lymph node metastasis status. Up-regulated KLF5 expression promoted the proliferation, migration, and invasion of ESCC cells. Reduced KLF5 showed the opposite effects. Mechanistically, KLF5 exerts its tumor promotion effect by up-regulating fibroblast growth factor binding protein 1 (FGF-BP1) and snail family transcriptional repressor 2 (SNAIL2). KLF5 binds to the promoter regions of FGF-BP1 and transcriptionally activates its expression. Our study indicated that KLF5 could promote esophageal squamous cell cancer proliferation, migration, and invasion by up-regulating FGF-BP1/ SNAIL2 signaling. Our work suggests that KLF5 might be a proto-oncogene in ESCC and implicated in ESCC metastasis.
Design and Stability Analysis of an Adaptive Neuro-Fuzzy Inference System (ANFIS) Based Pacemaker controller in MATLAB Simulink
Authors: Dabiriaghdam Asghar , Dabanloo Nader Jafarnia , Rahatabad Fereidoun Nooshiravan , Maghooli Keivan 
Published: April 2023

Abstract
This paper presents the design and stability analysis of an Adaptive neuro-fuzzy inference system-based controller of a pacemaker in MATLAB Simulink. ANFIS uses Learning and Speed properties of Fuzzy and Neural Networks. Based on body states and preprogrammed situations of patients (age and sex, etc.),heart rate and amplitude of pacing pulse are changed. Output signal that is fed backed from heart is compared to the reference fuzzy bases ANFIS signals .After designing ANFIS based controller, the stability of the proposed system has been tested in both Time (Step response) and Frequency domains(Bode Diagram and Nichols chart). In our previous paper Step response analyzed and compared with other works. For frequency domain, all the possible frequency analysis methods have been tested but because of nonlinear properties of ANFIS, after linearization, just the Bode diagram achieved good results. The step response results in time domain is compared with previous work's results including optimum heart pulse rate for each particular patient. In frequency-domain the Bode diagram stability analysis showed Gain and phase margin as follows: GM (dB) = 42.1 and PM (deg) = 100
The differences effect between the frequency of drinking telang flower tea and erobic exercise on weight loss and cholesterol
Authors: Purnomo Eddy , Kurniawan Dennis Dwi , Rizkyanto Willy Ihsan 
Published: April 2023

Abstract
Obesity and cholesterol are very feared problems. In recent years, many people have tried to use alternative treatments by consuming ingredients from plants, one of which is telang flower tea (Clitoria ternatea). The purpose of this study was to test and determine the difference in the effect of the frequency of tea drinks (Clitoria ternatea) accompanied by aerobic exercise on weight loss (Body Mass Index) and cholesterol. Experimental research design with pre and post-test design methods. Group grouping using matching pairing and data collection took measurements of body mass index, and blood tests in the form of cholesterol tests. The study population was all Military National Indonesian members who had a BMI > 25. The data analysis technique uses a difference test of 2 paired groups to distinguish the pretest and posttest in each group, with a significance level of 5%. The results of the analysis showed that: 1) there was an effect of the frequency of drinking 2 times the tea accompanied by aerobic exercise on the decrease in Body Mass Index (BMI) and cholesterol, 2) There was an effect of the frequency of drinking 5 times the tea accompanied by aerobic exercise on the decrease in Body Mass Index (BMI) and cholesterol, 3) There was a difference in the effect of the frequency of drinking 2 times and 5 times the tea accompanied by aerobic exercise on reducing Body Mass Index (BMI) and cholesterol.
Novel Neurodigital Interface Reduces Motion Sickness in Virtual Reality
Authors: Dopsaj Milivoj , Tan Wilhelmina , Perovic Vladimir , Stajic Zoran , Milosavljevic Nemanja , Paessler Slobodan , Makishima Tomoko 
Published: April 2023

Abstract
Virtual reality (VR) is a computer-created 3D environment with a focus on realistic scenes and pictures created for entertainment, medical and/or educational and training purposes. One of the major side effects of VR immersion reported in the scientific literature, media and social media is Visually Induced Motion Sickness (VIMS), with clinical symptoms such as disorientation, nausea, and oculomotor discomfort. VIMS is mostly caused by the discrepancy between the visual and vestibular systems and can lead to dizziness, nausea, and disorientation. In this study, we present one potential solution to motion sickness in VR and demonstrate significant reduction of nausea in VR users with META Quest 2 headsets when using a whole-body controller to experience VR in a more comfortable and immersive way.
A 23-year-old man with acute myocardial infarction and pulmonary embolism for STAB2 gene mutation
Authors: Li Yihui , Fu Ru , Zeng Jianwei , Liu Tao , Li Ruixuan , Jiang Weihong , Li Xiaogang 
Published: April 2023

Abstract
Background
Thrombophilia is the tendency to thrombosis due to various acquired or genetic factors. Genetic thrombophilia refers to thromboembolism as a result of mutations in physiological anticoagulant or procoagulant genes, where persistent elevated factor VIII (FVIII) levels are also a common genetic factor in high thrombotic propensity. STAB2 variants can lead to increased plasma levels of the von Willebrand Factor (VWF) and/or its binding partner the coagulation cofactor factor FVIII. Nevertheless, venous thromboembolism has been the main clinical manifestation of STAB2 gene variants. In this paper, we report an interesting case in which a young armed police officer suffering from a STAB2 gene variant presented with acute myocardial infarction and pulmonary embolism.
Case presentation
A young armed police officer underwent emergency percutaneous coronary intervention(PCI) for ST-segment elevation myocardial infarction(STEMI) and his pulmonary image indicated pulmonary embolism. After thrombophilia testing, the diagnosis of arteriovenous thrombosis due to the STAB2 gene mutation and smoking was finally confirmed.
Conclusion
The STAB2 gene variant can manifest as venous and acute arterial thromboembolism. Young patients with unexplained arteriovenous thrombosis should undergo rigorous laboratory tests and related genetic screening of thrombophilia. While other arteriovenous thromboses should be excluded and antithrombotic therapy initiated promptly.
Factors associated with obtaining prescribed safer supply among people accessing harm reduction services: findings from a cross-sectional survey
Authors: Palis Heather , Haywood Beth , McDougall Jenny , Xavier Chloe , Desai Roshni , Tobias Samuel , Burgess Heather , Fergusson Max , Liu Lisa , Kinniburgh Brooke , Slaunwhite Amanda , Crabtree Alexis , Buxton Jane 
Published: April 2023

Abstract
19 transmission among people who use drugs, in March 2020, prescribed safer supply guidance was released in British Columbia. This study describes demographic and substance use characteristics associated with obtaining prescribed safer supply and examines the association between last six month harm reduction service access and obtaining prescribed safer supply.
Methods: Data come from the 2021 Harm Reduction Client Survey administered at 17 harm reduction sites across British Columbia. The sample included all who self-reported use of opioids, stimulants, or benzodiazepines in the prior 3 days (N=491), given active use of these drugs was a requirement for eligibility for prescribed safer supply. The dependent variable was obtaining a prescribed safer supply prescription (Yes vs. No). The primary independent variables were access to drug checking services, and access to overdose prevention services in the last six months (Yes vs. No). Descriptive statistics (Chi-square tests) were used to compare the characteristics of people who did and did not obtain a prescribed safer supply prescription. Multivariable logistic regression models were run to examine the association of drug checking services and overdose prevention services access with obtaining prescribed safer supply.
Results: A small proportion (n=81(16.5%)) of the sample obtained prescribed safer supply. After adjusting for gender, age, and urbanicity, people who reported drug checking services access in the last six months had 1.67 (95% CI: 1.00-2.79) times the odds of obtaining prescribed safer supply compared to people who had not contacted these services, and people who reported last six months overdose prevention services access had more than twice the odds (OR: 2.08 (95% CI: 1.20-3.60)) of prescribed safer supply access, compared to people who did not access these services.
Conclusions: Overall, the proportion of respondents who received prescribed safer supply was low, suggesting that this intervention is not reaching all those in need. Harm reduction services may serve as a point of contact for referral to prescribed safer supply. Additional outreach strategies and service models are needed to improve the accessibility of harm reduction services and of prescribed safer supply in British Columbia.
Prevalence and Characterization of Long Covid-19 Symptoms in Health Care Professionals- A Need of the Hour
Authors: Srin Mote , Mehreen Syeda Fakiha , Kumar Surya Prem , Pavani Sanka , Rani Vannavada Sudha , Reddy Palkonda Shashikala 
Published: April 2023

Abstract

Background: With the global advent of Covid-19, Healthcare workers (HCW) were under a lot of physical and psychological pressure. Information on persistent symptoms post Covid-19 Infection in HCWs is lacking.
Objectives: This Study is aimed at assessing the impact of the post Covid-19 syndrome in HCWs.
Materials and Methods: A Questionnaire was prepared as google form and shared with the HCWs through WhatsApp enquiring regarding the health conditions that are still persistent post recovery from Covid-19 infection.
Results: A total of 328 Health Care Professionals participated in the present survey (18-65 yrs). The gender distribution revealed 67.7% were females and 32.3% were males. 60.3% of the participants were infected with COVID-19 before taking the first dose of vaccination which is reduced to 17.5% after vaccination. The post COVID complications observed from the study were hair loss (35.4%), easy fatigability (25%), mood swings (22.9%), anxiety (18.8%), insomnia/sleeplessness (13.9%), depression (12.5%) and joint pains/arthritis (11.8%). The other complications observed were loss of taste (9%), lightheadedness/postural hypotension (8.3%), amnesia/loss of memory and anosmia/loss of smell (7.6%), gastritis (6.3%), palpitations, hypersomnia and pulmonary complications (5.6%) and chest pain (4.9%). Unpaired t-test and One-Way ANNOVA resulted in a significant value (p values of >0.05).
Discussion: Despite the fact that females experienced more post-Covid-19 symptoms (15 out of 17), males experienced more chest pain and anxiety symptoms. According to our findings, 57 of 100 Covid-19 health care workers have post-Covid complications. The participants presented with non-specific symptoms such as easy fatigability, mood swings, light headedness, anxiety but most of the participants quoted more specific symptoms such as depression, pulmonary complications, hair loss, joint pains, gastritis, chest pain, palpitations, loss of taste, amnesia, hyperglycemia, insomnia, hypersomnia and anosmia. However, non-specific symptoms such as fatigue, mood swings, lightheadedness, and anxiety were also mentioned. The symptoms of post-acute COVID-19 syndrome vary greatly. Early detection requires a unified definition of long COVID and characterization of its manifestation. Furthermore, more research should be conducted to identify risk factors and the precise mechanisms that lead to the development of long COVID syndrome. Such knowledge may aid future research aimed at preventing such a complication.
Tribological behavior of steel-based cold spray composite coatings
Authors: AUBANEL Laurent , DELLORO Francesco 
Published: April 2023

Abstract
This study investigates the microstructure, the mechanical properties, the topography and the tribological behavior of a composite cold spray coating, consisting in a 410L matrix and M2 tool steel reinforcements. The surface finish creates protuberances due to the presence of M2 particles. A lubricated reciprocating sliding test was performed for the composite and for other two coatings, for comparison. The analyses revealed the presence of a tribofilm, leading to low friction and negligible wear even in harsh conditions. This study opens the interest for surface textures with protuberances and the use of cold spray process to easily produce them.
Randomized Controlled Trial of All-inside and Standard Anterior Cruciate Ligament Reconstruction about Knee Laxity, Tunnel Widening and MRI-based Fluid Signal within Tunnel
Authors: Li Wei , Cui Lei , Jiang Changqing , Lin Rubing , Zhong Qiuwen , Wu Xiao , Huang Rong , Deng Qianhua 
Published: April 2023

Abstract

Purpose:This study focused on the characteristics of the functional, knee laxity, tunnel widening and tunnel fluid signal of all-inside and standard single-bundle anterior cruciate ligament reconstruction (ACLR).Methods:This is a randomized controlled trial.From September 2018 to July 2019, 54 patients were enrolled and randomly assigned to all-inside group (n=27) and standard group (n=27). Followed up at postoperative 3rd, 6th, and 12thmonth, the International Knee Documentation Committee (IKDC), Visual Analogue Scale (VAS), Lysholm，and Tegner score were recorded. The tunnel widening and tunnel liquid signal were measured by CT and MRI. The knee laxity was assessed by GNRB arthrometer at postoperative 12thmonth.Results:The tibial tunnel widening at postoperative 3rd, 6th, and 12thmonth in all-inside group was significantly less than that in standard group (P < 0.05). There was no statistical difference in femoral tunnel widening between the 2 groups (P > 0.05). At postoperative 3rdand 6thmonth the liquid signal in tibial tunnel between the 2 groups had no statistical difference. The VAS score in all-inside group was lower than the standard group (P < 0.05). There was no significant difference in Tegner, IKDC, and Lysholm score between the 2 groups. At postoperative 12thmonth the knee laxity in all-inside group was higher than the standard group (P < 0.05).Conclusions:All-inside single-bundle ACLR can slow down tunnel widening, relieve postoperative pain, and achieve same functional recovery compared with the standard single-bundle ACLR but the knee laxity was relatively higher. Both techniques have the same fluid signal in tibial tunnel in the early postoperative period.
An Indonesian Adaptation of The Students’ Preparedness for University e-Learning Environment Questionnaire
Authors: Junus Kasiyah , Sadita Lia , Siswantining Titin , Vitasari Diana Nur 
Published: April 2023

Although most students are digital natives, online learning requires different skills as compared to conventional face-to-face learning. This paper aims to adapt and test the reliability and validity of the Students’ Preparedness for the University e-Learning Environment questionnaire developed by Parkes et al. into Bahasa Indonesia. The original questionnaire covers a wide range of competencies relevant to e-learning preparedness for university e-learning environments in three dimensions. Prior to reliability and validity checking, pilot testing is conducted to test the unidimesionality of the instrument and the rating scale. An item-match analysis test is also carried out to observe the suitability of each item. Then, the final version of the questionnaire is administered to a large representative sample of respondents for whom the questionnaire is intended. The results show that, with a total of 1446 students from a public university in Indonesia as respondents, the adapted questionnaire is valid and reliable.
Evaluating uncertainty in climate change impacts on peak discharge and flood volume in the Qaran Talar watershed, Iran
Authors: Imani Toktam , Delghandi Mahdi , Emamgholizadeh Samad , Ganji-Noroozi Zahra 
Published: April 2023

Abstract
This study evaluates the effects of climate change (CC) on runoff properties over the case study of the Qaran Talar watershed located in Iran. To consider the two main sources of uncertainty, i.e., Greenhouse Gases emission (GHG) scenarios and outputs of Atmosphere-Ocean General Circulation Models (AOGCMs), a daily rainfall time series was generated for two future periods (2021-2050 and 2070-2099) at three risk levels of 0.1, 0.25 and 0.50. 22 AOGCMs outputs following two emission scenarios (RCP4.5 and RCP8.5) were used. The results showed that the uncertainty of climate change scenarios was primarily owing to the uncertainty of GCMs outputs. Regarding the 2021-2050 period, under both emission scenarios, the increases in peak discharge and flood volume (FV) were estimated to reach 70, 50, and 30% at three risk levels of 0.1, 0.25 and 0.50, respectively, compared to the recent past period. As the current century draws to a close, the difference between the results of the two emission scenarios becomes apparent so that in the far-future period (2070-2099), the RCP8.5 scenario estimates and FV more than the RCP4.5 scenario does. Additionally, it was found that the uncertainty caused by AOGCMs was more than that by GHG emission scenarios.
Impacts of COVID-19 lockdowns on water quality along the coast of Morocco
Authors: Azidane Hind , Primadona Noir Purba , Boko Michel , ElBouhaddioui Mohammed , Magrane Bouchain 
Published: April 2023

Abstract
Due to the COVID-19 pandemic, most of the marine activities and all the anthropogenic activities along the coast have been shut off for several months. The total suspended matter (TSM) was analyzed before and during COVID-19 lockdown in the coast of Morocco. Therefore, the aim of this study was to assess all the changes reflected through the coast in April of 2018, 2019, 2020 and 2021. In the absence of the ground observations during this period, the remote sensing data was used in this study. The results showed a gradual reduction in TSM concentration, indicating a positive improvement during the lockdown period. The TSM concentrations during this period decreased by 40.59% on average compared with the pre-lockdown period. Further, the turbidity in the water has reduced at each stretch of the rivers. Remote sensing is a powerful tool for analyzing turbidity over the whole littoral areas, even in the absence of field observations. The ongoing pandemic shows that an ocean with cleaner water is possible. These findings provide a general reference on the state of the Moroccan coast that could contribute to improve policy and future monitoring program.