bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomechanics in the tumor microenvironment: from biological functions to potential clinical applications
Authors: Peng Hao , Chao Zheng , Wang Zefeng , Hao Xiaodong , Xi Zirui , Ma Sheng , Guo Xiangdong , Zhang Junbiao , Zhou Qiang , Qu Guanyu , Gao Yuan , Luo Jing , Wang Zhihua , Wang Jing , Li Le 
Published: January 2025

AbstractImmune checkpoint therapies have spearheaded drug innovation over the last decade, propelling cancer treatments toward a new era of precision therapies. Nonetheless, the challenges of low response rates and prevalent drug resistance underscore the imperative for a deeper understanding of the tumor microenvironment (TME) and the pursuit of novel targets. Recent findings have revealed the profound impacts of biomechanical forces within the tumor microenvironment on immune surveillance and tumor progression in both murine models and clinical settings. Furthermore, the pharmacological or genetic manipulation of mechanical checkpoints, such as PIEZO1, DDR1, YAP/TAZ, and TRPV4, has shown remarkable potential in immune activation and eradication of tumors. In this review, we delved into the underlying biomechanical mechanisms and the resulting intricate biological meaning in the TME, focusing mainly on the extracellular matrix, the stiffness of cancer cells, and immune synapses. We also summarized the methodologies employed for biomechanical research and the potential clinical translation derived from current evidence. This comprehensive review of biomechanics will enhance the understanding of the functional role of biomechanical forces and provide basic knowledge for the discovery of novel therapeutic targets.
Low impact of Zostera marina meadows on sediment and water microbiota under brackish conditions
Authors: Herlemann Daniel P. R. , Delgado Luis F. , Riedinger David J. , Fernández-Juárez Víctor , Andersson Anders F. , Pansch Christian , Riemann Lasse , Bengtsson Mia M. , Gyraitė Greta , Kataržytė Marija , Kisand Veljo , Kube Sandra , Martin Georg , Piwosz Kasia , Rakowski Marcin , Labrenz Matthias 
Published: January 2025

Abstract
Background
Zostera marina is an important ecosystem engineer influencing shallow water environments and possibly shaping the microbiota in surrounding sediments and water. Z. marina is typically found in marine systems, but it can also proliferate under brackish conditions. Changes in salinity generally have a strong impact on the biota, especially at the salty divide between salinity 6 and 9. To better understand the impact of the salty divide on the interaction between Z. marina and the surrounding sediment and water microbiota, we investigated the effects of Z. marina meadows on the surrounding microbiota across a salinity range of 6–15 in the Baltic Sea during the summer using 16S and 18S rRNA gene amplicon sequencing.

Results
Salinity was the most important factor for structuring the microbiota within both water and sediment. The presence of Z. marina affected the composition of the bacterial and eukaryotic community and bacterial alpha diversity in the sediment. However, this effect was confined to alpha-mesohaline conditions (salinity 9–15). The impact of Z. marina below salinity 9 on water and sediment microbiota was insignificant.

Conclusions
Increasing salinity was associated with a longer leaf length of Z. marina, causing an increased canopy height, which affects the sediment microbiota through reduced water velocity. Hence, we propose that the canopy effect may be the major predictor explaining Z. marina’s interactions with the surrounding microbiota at salinity 9–15. These findings emphasize the importance of the physical effects of Z. marina meadow ecosystem services and have important implications for Z. marina management under brackish conditions in a changing climate.
Multivariate GARCH models with spherical parameterizations: an oil price application
Authors: Ballestra Luca Vincenzo , De Blasis Riccardo , Pacelli Graziella 
Published: January 2025

Abstract
In popular Baba-Engle-Kraft-Kroner (BEKK) and dynamic conditional correlation (DCC) multivariate generalized autoregressive conditional heteroskedasticity models, the large number of parameters and the requirement of positive definiteness of the covariance and correlation matrices pose some difficulties during the estimation process. To avoid these issues, we propose two modifications to the BEKK and DCC models that employ two spherical parameterizations applied to the Cholesky decompositions of the covariance and correlation matrices. In their full specifications, the introduced Cholesky-BEKK and Cholesky-DCC models allow for a reduction in the number of parameters compared with their traditional counterparts. Moreover, the application of spherical transformation does not require the imposition of inequality constraints on the parameters during the estimation. An application to two crude oils, WTI and Brent, and the main exchange rate prices demonstrates that the Cholesky-BEKK and Cholesky-DCC models can capture the dynamics of covariances and correlations. In addition, the Kupiec test on different portfolio compositions confirms the satisfactory performance of the proposed models.
The Möbius Paradigm: A transformative approach for integrating stakeholder perspectives when navigating dilemmas in complex operating environments
Authors: O’Riordan Linda , Hampden-Turner Charles 
Published: January 2025

AbstractAmidst the growing complexity and uncertainty of today’s operating environments, traditional binary thinking—reducing choices to limited “either/or” decisions—often falls short. To address these nuanced challenges and the conflicting opportunities and threats shaped by diverse stakeholder perspectives, a radically more sophisticated approach is needed. This paper introduces The Möbius Paradigm, a transformative approach which transcends outdated paradigms through systems thinking. By embracing Möbius infinity loop pathways, leaders inspired by radical empathy can transcend simplistic dichotomies, adopting a more holistic and sustainable framework for resolving complex dilemmas. The study highlights the critical challenges faced by choice-makers navigating diverse perspectives within complicated environments, emphasising dynamic stakeholder relationship management as essential for successful leadership. It offers practical examples of how Möbius Mindsets, Logic, and Thinking can be applied to multifaceted scenarios, fostering inclusive, adaptive, and conscious solutions. By integrating awareness of underlying values in choice-making, the Möbius Paradigm enables profound change, bridging the major divides of our time. This approach promotes the realisation of sustainable outcomes, enhancing well-being in business and beyond.
Comparing the effect of addition of different supplements to sperm preparation media on intracytoplasmic injection outcome
Authors: Adly Mohamed Ashraf , Saad Ahmed Samy , Abou-El-Naga Amoura 
Published: January 2025

Abstract
Background
To assess the effectiveness of human sperm treated with an egg yolk-enhanced refrigeration group and to determine the protective effect of selenium supplementation in a laboratory setting, samples were prepared using the density gradient method as the standard preparation technique. Additionally, the study aimed to investigate how these treatments affected the outcomes of intracytoplasmic sperm injection (ICSI). This randomized, double-blind, placebo-controlled clinical trial involved 263 couples seeking treatment for male infertility between December 2021 and December 2023. Couples pursuing ICSI for primary or secondary infertility were included in the study. The outcomes of the ICSI procedure were evaluated across the treatment groups.

Results
No statistically significant differences were observed between the density gradient group and the refrigeration group in terms of the number of injected oocytes, fertilized oocytes, or fertilization rates. However, the refrigeration group showed a significantly higher number of top-cleaved oocytes and a greater top cleavage rate compared to the density gradient group (p = 0.02). Additionally, the refrigeration group had a significantly higher number of transferred embryos (p < 0.001). Similarly, the selenium group demonstrated a significantly higher number of top-cleaved oocytes and a greater top cleavage rate than the density gradient group (p = 0.04 and p < 0.001, respectively). The number of transferred embryos was also significantly higher in the selenium group compared to the density gradient group.

Conclusions
Both refrigeration with an egg yolk buffer and selenium supplementation had significant positive effects on oocyte maturation and embryo development, resulting in higher top cleavage rates and an increased number of transferred embryos. However, pregnancy rates following each technique individually were comparable to those achieved with the density gradient method.
Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties
Authors: Devan Elumalai , Maduraiveeran Hemavathi , Raja Kavitha , Chinnasamy Arulvasu , Sivalingam Govindan , Balaji Sathya , Neelakandan Vinoth , Agnita Sharon , Rajaram Murugan 
Published: January 2025

Abstract
Background
The mucus from fish and gastropods contains a wide range of bioactive molecules with biomedical properties. The fish and gastropods were collected from Oragadam lake, Kanchipuram district, Tamil Nadu, India. In this study, we wanted to examine the anticancer potential of fish and gastropods mucus. The biochemical components of the crude mucus were screened.

Results
The biochemical analysis showed that the mucus of Anabas testudineus and Pila virens contained a high level of carbohydrates (2.8 and 1.5 mg/ml), the mucus of Channa striata contained a high level of lipids (0.9 mg/ml), and the mucus of Helix aspersa contained a high level of protein (1.3 mg/ml). The results showed morphological variations in the HT-29 cells upon treatment with crude mucus. Upon 24 h of gestation, the frozen cells began to shrink and seem round in shape. Using the MTT assay, the mucus crude extract was evaluated for its anticancer properties against the human colon cancer cell line (HT 29). The inhibitory concentration (IC50) was determined at 100 µg/ml after 24 h. Using specific staining techniques; fluorescent microscopy was utilized to examine the cell morphology and early and late apoptotic stages. Propidium iodide staining showed nuclear damage followed by DNA damage. This showed that the rudimentary mucus could prompt cell death and increased the number of fragments and mucus concentration, respectively.

Conclusion
This study showed that the edible or commercially important fish and gastropod mucus have potential anticancer activity against HT-29 cancer cells.
Effects of adjuvant hyperbaric oxygen therapy and real-time fluorescent imaging on deep sternal wound infection: a retrospective study
Authors: Li Tse-Hsi , Lin Cheng-Hsin , Peng Chung-Kan , Wu Yi-Chun , Hsieh Tsung-Cheng , Lee Chi-Hung , Liu Yung-Ching , Huang Kun-Lun , Tam Ka-Wai , Chang Shun-Cheng 
Published: January 2025

Objective: Deep sternal wound infection (DSWI) is a rare but devastating complication that is estimated to occur in 1–2% of patients after median sternotomy. Current standard of care (SoC) comprises antibiotics, debridement and negative pressure wound therapy (NPWT). Hyperbaric oxygen therapy (HBOT) appears to be an effective adjuvant therapy for osteomyelitis. The aim of this study was to determine the effectiveness of HBOT and real-time fluorescence imaging (RTFI) in a DSWI treatment protocol and their benefits on infection control. Method: A retrospective analysis of DSWI management was performed. Enrolled patients were divided into two groups: HBOT group and RTFI group. Patients in the HBOT group received SoC, HBOT, NPWT and reconstructive flap surgery. Patients in the RTFI group received the same therapeutic plan as well as treatment with a RTFI device (MolecuLight i:X (MolecuLight, Inc., Canada) to achieve high-quality debridement. Infection status and short-term outcomes within three months were measured. Long-term outcomes were analysed at a 12-month follow-up. Results: Of the 55 patients enrolled: 22 in the HBOT group and 33 in the RTFI group. Infection control status, evaluated in terms of white blood cell counts and C-reactive protein levels, antibiotic use duration, antibiotic costs, reinfection rate and osteomyelitis recurrence rate, were statistically significantly improved in the RTFI group (<0.001, <0.001, 0.042, 0.022, 0.049 and 0.022, respectively). Length of total intensive care unit stay and duration of complete healing were statistically significantly decreased in the RTFI group (<0.001 and 0.046, respectively). Conclusion: Patients with DSWI can benefit from HBOT, especially in terms of in-hospital mortality. RTFI can be used to eliminate bacterial burden and achieve high-quality debridement, which considerably improves infection control and clinical outcomes.
Gentamicin ointment effect on hard-to-heal wounds: a case series
Authors: Cheney Mary , Pei Mitchell , Zajac Kelsee , Womack Braden , Workman Lauren , Schubauer Kathryn , Simman Richard 
Published: January 2025

Objective: The presence of microorganisms in a wound may lead to the development of pathologically extensive inflammation, and either delay or prevent the healing of hard-to-heal (chronic) wounds. The aim of this case series is to explore the use of topical gentamicin ointment, an aminoglycoside with activity against aerobic Gram-negative bacteria, as an option to address hard-to-heal wounds. Method: We present a retrospective case series of patients with hard-to-heal wounds of varying pathophysiologies treated with topical gentamicin. Results: Regardless of the inciting aetiology, all 10 of the presented patients responded to topical gentamicin as evidenced by resolution of much of the inflammation and reduction in wound size or complete wound closure. Conclusion: By helping to clear the bacteria-induced biofilm that may be present on a hard-to-heal wound, gentamicin may allow the wound healing process to continue past the inflammatory phase, and onto proliferation, remodelling and eventual closure.
Cerebral complications after unilateral biportal endoscopic surgery: A case report
Authors: Han Chao , Ren Zhan-Yun , Jiang Zhen-Huan , Luo Yi-Feng 
Published: January 2025

BACKGROUND
Unilateral biportal endoscopic (UBE) surgery has developed rapidly during the past decade. Continuous epidural space irrigation is generally considered the principal reason for cerebral complications following UBE surgery. We present a case of mental symptoms during the general anesthesia awakening period due to pneumocephalus.
CASE SUMMARY
A 70-year-old woman with lumbar disc herniation underwent UBE surgery stably under general anesthesia. Uncontrollable hypertension occurred immediately after transfer to the postoperative care unit, accompanied by increased heart rate and tachypnea. During the recovery process, the patient responded to external stimuli but was confused and unable to complete command actions. Neck stiffness and significantly increased muscle strength on the left side indicated the presence of de-cerebrate rigidity. An urgent brain computed tomography scan showed pneumocephalus compressing the brainstem. After receiving analgesia and sedation treatment, the patient was conscious three hours later and recovered rapidly. She was discharged on the fifth postoperative day and followed up for 3 months with no surgical or brain complications.
CONCLUSION
Cerebral complications emerging during the general anesthesia awakening period following UBE surgery are not entirely due to increased intracranial pressure. Pneumocephalus induced by dural injury may also be a potential cause.
Aquaculture infection of Kappaphycus alvarezii by Vibrio alginolyticus causing ice-ice disease
Authors: PRASETYO ENDRY NUGROHO , PERMANASARI YUNITA , BASYIRAH AFRA , FITRIYA SALSABILA AIDA , JADID NURUL , ZULLAIKAH SITI , EKAWATI IDA , ISDIANTONI ISDIANTONI , KOENTJORO MAHARANI PERTIWI 
Published: January 2025

Abstract. Prasetyo EN, Permanasari Y, Basyirah A, Fitriya SA, Jadid N, Zulaikah S, Ekawati I, Isdiantoni, Koentjoro MP. 2024. Aquaculture infection of Kappaphycus alvarezii by Vibrio alginolyticus causing ice-ice disease. Biodiversitas 25: 4086-4094. The occurrence of ice-ice disease on seaweed (Kappaphycus alvarezii (Doty) Doty ex P.C.Silva) tissue is indicated by the presence of bleaching and softening symptoms on thallus, which attracts microbial infection. In addition, it is often caused by unfavorable environmental conditions during specific seasons and is characterized by fragmentation as well as a reduction in biomass. To prevent the disease, several studies have suggested the use of ice-ice disease-resistant seeds. Therefore, the aim of this study was to investigate the resistance level of seaweed seeds to microbial infection, particularly Vibrio alginolyticus, which is responsible for ice-ice disease in different aquaculture areas in Sumenep District. Data were collected from four different villages, including Brakas, Padike, Tanjung, and Lobuk, followed by analysis using descriptive qualitative method. K. alvarezii seeds collected from four villages were infected with V. alginolyticus using the in vitro method. Thallus of seaweed was then observed and water samples were taken every 6 hours for 24 hours post-infection. The results showed that seeds from Brakas had the lowest (11%) infection rate, followed by Tanjung (67%), and Padike and Lobuk (89% each). This indicated that K. alvarezii seeds from Brakas possessed a prominent activity against V. alginolyticus infection with no sign of chlorosis or tissue softening on thallus.
New record and potential spatial distribution of Curcuma sumatrana (Zingiberaceae): An endemic wild turmeric in Sumatra, Indonesia
Authors: SYAFIRA FARADILA , NURAINAS NURAINAS , SYAMSUARDI SYAMSUARDI 
Published: January 2025

Abstract. Syafira F, Nurainas, Syamsuardi. 2024. New record and potential spatial distribution of Curcuma sumatrana (Zingiberaceae): An endemic wild turmeric in Sumatra, Indonesia. Biodiversitas 25: 4127-4138. Curcuma sumatrana Miq. is a Sumatran turmeric species with medicinal potential. However, it remains underutilized and is classified as vulnerable by the IUCN. Its vulnerability is aggravated by limited knowledge of its distribution, a need for more data on habitat preferences, and habitat degradation. Field observations revealed significant morphological variations among populations, likely influenced by environmental factors. This study assessed the morphology, microhabitat preferences, and spatial distribution of C. sumatrana in West Sumatra, Indonesia. Surveys and laboratory observations highlighted morphological traits, such as leaf and ligule length and width, significantly contribute to these observed variations. Notably, the leaf shape differed between open and shaded areas, being narrowly elliptic in open areas and broadly elliptic in shaded areas. Populations in Koto Malintang and Lubuk Minturun showed distinct differences, separated by six morphological traits. The C. sumatrana prefers habitats with fertile soils, moderate plant diversity, and open land cover, often coexisting with species like Dendrocnide stimulans (L.fil.) Chew, Macaranga tanarius (L.) Müll.Arg., and Diplazium sp. Maximum Entropy modeling (AUC 0.944) predicted a highly suitable habitat of 918 hectares in the western Bukit Barisan range. The model suggests distribution is influenced by soil type and precipitation patterns during the seasonal, warmest, and coldest quarters, as well as land cover. New records from West Sumatra extend the species' known range, reaffirming its vulnerable status with a potential risk of becoming endangered.
Spectropolarimetric properties of vortex retarders: erratum
Authors: James Ella E. , Anderson Isaac M. , Gregory Don A. 
Published: January 2025

After publication of [Opt. Express 32, 30124 (2024)10.1364/OE.524955], the authors noticed that in Figs. 8, 10, 12, and 14, the plot labels for "Diattenuator Orientation" and "Circular Diattenu- ation" were swapped. The values displayed on the figures are correct; however, the middle subfigure should be labeled, "Circular Diattenuation," and the middle right subfigure should be labeled, "Diattenuator Orientation." This does not affect the main conclusions of the paper in any way, since the paper focuses primarily on retardance.
Nisi paria non pugnant: argument structure alternations with lexical reciprocal verbs in Latin
Authors: Inglese Guglielmo 
Published: January 2025

Abstract
Lexical reciprocal verbs, defined as those verbs that inherently lexicalize a symmetric situation, e.g., consentio ‘agree’, occur in at least three distinct argument structure constructions, depending on whether their participants are expressed as a single (plural) subject, asymmetrically expressed – one as subject and the other one as oblique – or only one is overtly realized. Drawing from extensive data from a corpus of Latin texts (extracted from the LASLA corpus) and by resorting to quantitative analyses, I investigate the extent to which these three constructions are used with a sample of lexical reciprocal verbs and explore possible motivations for the alternation. Corpus data shows that a multiplicity of factors co-operate in motivating speakers’ choice of one construction over the other, pointing to the need to integrate fine-grained corpus analyses in the study of argument structure constructions and their alternations more generally.
Particle dynamics in optical tweezer systems
Authors: Wu Xinxin , Liu Yueyan , Jin Shangzhong , Yu Mingzhou 
Published: January 2025

Abstract
The last four decades have witnessed the flourished harvesting in optical tweezers technology, leading to the development of a number of mainstream and emerging disciplines, particularly in physico-chemical processes. In recent years, with the advancement of optical tweezers technology, the study of particle dynamics has been further developed and enhanced. This review presents an overview of the research progress in optical tweezers from the perspective of particle dynamics. It cites relevant theoretical models and mathematical formulas, delves into the principles of mechanics involved in optical tweezers technology, and analyzes the coupling of the particle force field to the optical field in a continuous medium. Through a review of the open literature, this paper highlights historical advances in research on the dynamical behavior of particles since the invention of optical tweezers, including diffusion, aggregation, collisions, and fluid motion. Furthermore, it shows some specific research cases and experimental results in recent years to demonstrate the practical application effects of the combination of particle dynamics and optical tweezers technology in several fields. Finally, it discusses the challenges and constraints facing the field of combining particle technology with optical tweezers technology and prospects potential future research directions and improvements.