bluuebunny/crossref_metadata_2025_split huggingface dataset:
Organotypic Hippocampal Culture Model Reveals Differential Responses to highly similar Zika virus isolates
Authors: Oliveira Marina da Silva , Cassiano Larissa Marcely Gomes , Pioline Jeanne , Carvalho Ketyllen Reis Andrade , Salim Anna Christina Matos , Alves Pedro Augusto , Fernandes Gabriel Rocha , Machado Alexandre de Magalhães Vieira , Coimbra Roney Santos 
Published: February 2023

Abstract
Introduction

Zika virus (ZIKV) caused an outbreak in Brazil, in 2015, being associated to microcephaly. ZIKV has a strong neurotropism leading to death of infected cells in different brain regions, including the hippocampus, a major site for neurogenesis. The neuronal populations of the brain are affected differently by ZIKV from Asian and African ancestral lineages. However, it remains to be investigated whether subtle variations in the ZIKV genome can impact hippocampus infection dynamics and host response.

Objective

This study evaluated how two Brazilian ZIKV isolates, PE243 and SPH2015, that differ in a single missense amino acid substitution in the protein NS1, affect the hippocampal phenotype and transcriptome.

Methods

Organotypic hippocampal cultures (OHC) from infant Wistar rats were infected with PE243 or SPH2015 and analyzed in time series using immunofluorescence, confocal microscopy, RNA-Seq and RT-qPCR.

Results

Unique patterns of infection and changes in neuronal density in the OHC were observed for PE243 and SPH2015 between 8 and 48 hours post infection (p.i.). Phenotypic analysis of microglia indicated that SPH2015 has a greater capacity for immune evasion. Transcriptome analysis of OHC at 16 hours p.i. disclosed 32 and 113 differentially expressed genes (DEGs) in response to infection with PE243 and SPH2015, respectively. Functional enrichment analysis suggested that infection with SPH2015 activates mostly astrocytes rather than microglia. PE243 downregulated biological process of proliferation of brain cells and upregulated those associated with neuron death, while SPH2015 downregulated processes related to neuronal development. Both isolates downregulated cognitive and behavioral development processes. Ten genes were similarly regulated by both isolates. They are putative biomarkers of early hippocampus response to ZIKV infection. At 5, 7, and 10 days p.i., neuronal density of infected OHC remained below controls, and mature neurons of infected OHC showed an increase in the epigenetic mark H3K4me3, which is associated to a transcriptionally active state. This feature is more prominent in response to SPH2015.

Conclusion

Subtle genetic diversity of the ZIKV affects the dynamics of viral dissemination in the hippocampus and host response in the early stages of infection, which may lead to different long-term effects in neuronal population.
Rheological and spectroscopic study of luminescent Eu3+ -doped gellan gum
Authors: Torres Francisco R. , Sarmento Victor Hugo V. , Barud Hernane S. , Caiut José Maurício A. 
Published: February 2023

Abstract
Gellan gum (GG) can be prepared in the form of a gel and be used as a versatile and functional biomaterial. GG can come in the acetylated (HAGG) and deacetylated (LAGG) forms. There are gaps in the study of these GG matrixes associated with lanthanide ions, well-known for their luminescent properties. In this work, we have doped HAGG and LAGG with Eu3+ ions and used the resulting HAGG_Eu and LAGG_Eu gels as structural probe in spectroscopic analyses. The presence of Eu3+ ions in the GG polymeric network gradually changed the viscoelastic properties of the HAGG_Eu and LAGG_Eu gels, as confirmed by oscillatory rheology analysis. The spectroscopic study of the films obtained from HAGG_Eu and LAGG_Eu revealed how the Eu3+ ion interaction occurs, thereby improving our understanding about their structures. To intensify the emission process in the LAGG_Eu films, we added the β-diketone ligand TTA (2-thenoyltrifluoroacetone) to them, that acted as a sensitizer for Eu3+ ions. In conclusion, we have been able to obtain luminescent films based on GG. In addition, we have carried out an unprecedented structural study of the Eu3+-containing films, which have promising luminescent properties for use as new biocompatible systems.
Incidence of Intraoperative Hypotension during Non-cardiac Surgery in Community Anesthesia Practice: A retrospective observational analysis
Authors: Saasouh Wael , Christensen Anna L. , Xing Fei , Chappell Desirée , Lumbley Josh , Woods Brian , Mythen Monty , Dutton Richard P. 
Published: February 2023

Abstract
Background: Intraoperative hypotension (IOH) is well-described in the academic setting but not in community practice. IOH is associated with risk of postoperative morbidity and mortality. This is the first report of IOH in the community setting using the IOH measure definition from the Centers for Medicare & Medicaid Services Merit-based Incentive Payment System program. Objectives: To describe the incidence of IOH in the community setting; assess variation in IOH by patient-, procedure-, and facility-level characteristics; and describe variation in risk-adjusted IOH across clinicians.
Methods: Design: Cross-sectional descriptive analysis of retrospective data from anesthesia records in 2020 and 2021. Setting: Forty-five facilities affiliated with two large anesthesia providers in the U.S. Participants: Patients aged 18 years or older having non-emergent, non-cardiac surgery under general, neuraxial, or regional anesthesia. Cases were excluded based on criteria for the IOH measure: baseline mean arterial pressure (MAP) below 65 mmHg prior to anesthesia induction; American Society of Anesthesiologists (ASA) physical status classification of I, V, or VI; monitored anesthesia care only; deliberate induced hypotension; obstetric non-operative procedures; liver or lung transplant; cataract surgery; non-invasive gastrointestinal cases. Main Outcomes: IOH, using four definitions. Primary definition: binary assessment of whether the case had MAP < 65 mmHg for 15 minutes or more. Secondary definitions: total number of minutes of MAP < 65 mmHg, total area under MAP of 65 mmHg, time-weighted average MAP < 65 mmHg.
Results: Among 127,095 non-emergent, non-cardiac cases in community anesthesia settings, 29 percent had MAP < 65 mmHg for at least 15 minutes cumulatively, with an overall mean of 12.4 minutes < 65 mmHg. IOH was slightly more common in patients who were younger, female, and ASA II (versus III or IV); in procedures that were longer and had higher anesthesia base units; and in ambulatory surgery centers. Incidence of IOH varied widely across individual clinicians in both unadjusted and risk-adjusted analyses.
Conclusion: Intraoperative hypotension is common in community anesthesia practice, including among patients and settings typically considered “low risk.” Variation in incidence across clinicians remains after risk-adjustment, suggesting that IOH is a modifiable risk worth pursuing in quality improvement initiatives.
WITHDRAWN: Polypeptide-coated 5-FU/ICG co-delivery vehicle MSNs for cancer treatment with chemo/photothermal/photodynamic simultaneous therapy
Authors: Song Ping , Xu Guanglin , Gui Lin , Peng Guanglan , Li Wanzhen , Li Wenlong , Zhu Longbao , Zhang Weiwei , Ge Fei , Tao Yugui 
Published: February 2023

Abstract
Advances in material science, nanotechnology and biomedicine have rewritten many cancer treatment paradigms. The combined strategies based on nanomaterials for cancer treatment not only improve the efficacy of cancer treatment, but also avoid the limitations of traditional single cancer treatment. In this study, a novel nanoparticle 5-FU/ICG@MP has been synthesized, which is loaded with photosensitizer ICG and chemotherapeutic drug 5-FU using a mesoporous silica nanocarrier modified with amphiphilic polypeptide P14. The performance and surface morphology of the nanoparticles have been determined. The results indicated that the nanoparticles exhibit good dimensional stability, photothermal properties and efficient ROS generation. The in vitro anticancer activity of the nanoparticles was investigated based on cytotoxicity, apoptosis, live and dead staining, cell cycle and cell ultrathin section analysis. The results suggested that nanoparticles could effectively inhibit the cell activity of MCF-7 through chemotherapy combined with PDT and PTT. Finally, HE and TUNEL staining was used to analyze mouse organs and tumors. The nanoparticles induced apoptosis of tumor cells in vivo via multiple interaction between 5-FU and near infrared light triggered PTT and PDT. Therefore, the nanoparticles are effective drug delivery platform, which could simultaneously exerted chemo/photothermal/photodynamic treatment in furture.
Bioinformatic analysis of the potential common pathogenic mechanisms for psoriasis and metabolic syndrome
Authors: Zhou Yang , Han Lu , Wang Ziting , Fang Runan , Wan Yue , Yang Zeyu , Guan Ning , Li Jianhong 
Published: February 2023

Abstract
The pathogenesis of psoriasis and metabolic syndrome is relevant. However, the underlying biological mechanisms are not clear. A psoriasis training set was downloaded from the Gene Expression Omnibus (GEO) and was analyzed to identify differentially expressed genes (|log FC| > 1 and adjusted P-value < 0.05). Differentially expressed genes of metabolic syndrome were obtained from the GeneCards, Online Mendelian Inheritance in Man, and DisGeNET databases. Crosstalk genes were obtained for multiple enrichment analysis after identifying the disease intersection. Significant crosstalk genes were screened with the least absolute shrinkage and selection operator regression model and random forest tree model. Genes with area under the receiver operating characteristic curve (AUC) > 0.7 were selected for validation with two validation sets. Differential analyses of immune cell infiltration were performed on psoriasis lesion and control samples by the CIBERSORT and ImmuCellAI methods, and correlation analyses were performed between the screened significant crosstalk genes and immune cell infiltration. Significant crosstalk genes were analyzed based on the psoriasis area, severity index, and their responses to biological agents. Five signature genes (NLRX1, KYNU, ABCC1, BTC, and SERPINB4) were screened based on two machine learning algorithms, and NLRX1 was validated. The infiltration of multiple immune cells in psoriatic lesions and non-lesions was associated with NLRX1 expression. NLRX1 was found to be associated with psoriasis severity and response rate after the use of biologics. In conclusion, NLRX1 can potentially be a significant crosstalk gene for psoriasis and metabolic syndrome.
The Effect of Polyvinyl Alcohol on the Multicomponent Biopolymer/ Hydroxyapatite/Carbonated Hydroxyapatite Composite
Authors: Sari Yessie W. , Munir Ryaas M. , Saputra Angga , Sari Mona , Aminatun Aminatun , Suciati Tri , Yusuf Yusril 
Published: February 2023

Abstract
In this study, the synthesis of composite biomaterials consisting of hydroxyapatite (HA)/carbonated hydroxyapatite (CHA) with polymeric porous scaffolds is reported. Multicomponent biodegradable polymers were applied as the scaffold. These consist of alginate and chitosan. The effect of polyvinyl alcohol (PVA) involvement in the multicomponent polymeric scaffold was also investigated. The scaffold was loaded with HA/CHA, as bone graft materials, at various compositions. Asian moon scallop shell (Amusium pleuronectes) was used as a calcium biogenic resource for HA and CHA synthesis The effect of the PVA and HA/CHA concentration was investigated. In the absence of the PVA, the morphological study showed that the samples had porous structures with good pore interconnectivity. The XRD analysis showed that PVA reduced the crystallinity of the bone graft. The HA/CHA crystals were homogeneously dispersed. The introduction of PVA reduced the pore size and the porosity but provide stiffer materials, indicating that PVA may improve the mechanical properties of the composite. Some samples also showed the presence of small and large pores. The weight percentage content of HA or CHA influenced the crystallinity, indicating the possibility for the fabrication of tailored bone substitute materials.
Collision tumor of a thyroid papillary and follicular carcinoma: A case report
Authors: Kawasaki Kanako , Kai Keita , Tanaka Nariyuki , Kido Shinichi , Ibi Arisa , Minesaki Akimichi , Yamauchi Moriyasu , Kuratomi Yuichiro , Aishima Shinichi , Nakashima Masahiro , Ito Masahiro 
Published: February 2023

Abstract
Background: Papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC) are common differentiated thyroid cancers, but the detection of a collision tumor is an extremely rare event.
Case Presentation: The patient was a 69-year-old Japanese female with multiple cervical lymph node swellings and a thyroid tumor. Preoperative fine needle aspiration cytology of the enlarged lymph node revealed a cytological diagnosis of papillary thyroid carcinoma (PTC). A total thyroidectomy, right cervical dissection and paratracheal dissection were performed. Histopathological immunohistochemistry (IHC) analyses of resected specimens revealed a collision tumor of PTC and FTC. Multiple metastases of papillary carcinoma were found in the dissected lymph nodes. In the PTC lesion, IHC for BRAF (V600E) was positive but negative for the FTC lesion. Genetic analyses further revealed a TERT prompter C228T mutation in PTC and a NRAS codon 61 mutation in FTC. The patient died of recurrent cancer 8 months after surgery.
Conclusions: A case of a collision tumor of PTC and FTC is very rare, and even fewer cases have been subjected to genetic scrutiny. The present case was successfully diagnosed by pathological examination using IHC and genetic analyses. The TERT promoter mutation in the PTC lesion was consistent with the aggressive behavior of the cancer.
Going the Distance in Acoustic Neuroma Resection: Microsurgical Outcomes at High-Volume Centers of Excellence
Authors: Brandel Michael G. , Plonsker Jillian H. , Khan Usman A. , Rennert Robert C. , Friedman Rick A. , Schwartz Marc S. 
Published: February 2023

Abstract
Purpose: High-volume hospitals are associated with improved surgical outcomes for acoustic neuromas (ANs). Due to the benign and slow-growing nature of ANs, many patients travel to geographically distant cities, states, or countries for their treatment. However, the impact of travel burden to high-volume centers, as well as its relative benefit are poorly understood. We compared post-operative outcomes between AN patients that underwent treatment at local, low-volume hospitals with those that traveled long distances to high-volume hospitals.
Methods: The National Cancer Database was used to analyze AN patients that underwent surgery (2004–2015). Patients in the lowest quartile of travel distance and volume (Short-travel/Low-Volume: STLV) were compared to patients in the highest quartile of travel distance and volume (Long-travel/High-Volume: LTHV). Only STLV and LTHV cases were included for analysis.
Results: Of 13,370 cases, 2,408 met inclusion criteria. STLV patients (n = 1,305) traveled a median of 6 miles (Interquartile range [IQR] 3-9) to low-volume centers (median 2, IQR 1-3 annual cases) and LTHV patients (n = 1,103) traveled a median of 143 miles [IQR 103-230, maximum 4,797] to high-volume centers (median 34, IQR 28–42 annual cases). LTHV patients had lower Charlson/Deyo scores (p=0.001), mostly received care at academic centers (81.7% vs 39.4%, p<0.001), and were less likely to be minorities (7.0% vs 24.2%, p<0.001) or underinsured (4.2% vs 13.8%, p<0.001). There was no difference in average tumor size.
On multivariable analysis, LTHV predicted increased likelihood of gross total resection (odds ratio [OR] 5.6, 95% confidence interval [CI] 3.8-8.4, p<0.001), longer duration between diagnosis and surgery (OR 1.3, 95% CI 1.0-1.6, p=0.040), decreased length of hospital stay (OR 0.5, 95% CI 0.4-0.7, p<0.001), and greater overall survival (Hazard Ratio [HR] 0.6, 95% CI 0.4-0.95, p=0.029). There was no significant difference in 30-day readmission on adjusted analysis.
Conclusion: Although traveling farther to high-volume centers increased the time between diagnosis and treatment for AN patients, they experienced superior postoperative outcomes compared to patients who received treatment locally at low-volume centers. Enabling access and travel to high-volume centers may improve AN patient outcomes.
Acute Epstein–Barr virus positive cytotoxic T/Nature Killer cell lymphoid hyperplasia (AEBV+CT/NK-LH) mimicking natural killer/T cell lymphoma
Authors: Zhang Yanlin , Xie Jianlan , Zheng Yuanyuan , Zhou Xiaoge 
Published: February 2023

Abstract
To describe the clinicopathological features of seven patients with acute Epstein–Barr virus (EBV)-positive cytotoxic T cell/ natural killer lymphoid hyperplasia (AEBV + CT/NK-LH) in the lymphnode, in which initial findings led to a preliminary misdiagnosis of extranodal NK/T cell lymphoma(NKTL). Seven cases in children and young people from China with AEBV + CT/NK-LH were retrospectively analyzed. The patients were healthy prior to illness. Sudden onset occurred in all patients, with high fever as the first symptom followed by lymphadenopathy and hepatosplenomegaly. The disease courses before diagnosis were less than 1.5 months. The majority of lymphocytes in the lesions expressed CD3 and Granzyme B or TIA-1, but not CD5 in all cases. CD56 was expressed in numerous cells in 5 of 7 cases. EBER was detected in medium to large-sized cells (50–100 cells per high-power field) in all cases. TCR gene rearrangement was observed in six cases, among which monoclonal rearrangement was found in four cases. All patients received conservative treatment, but not chemotherapy. Four patients underwent anti-HLH therapy, and the others received anti-inflammatory treatment. All patients survived. All patients underwent long-term clinical observation and follow–up and did not relapse. Therefore, AEBV + CT/NK-LH could elicit malignant features mimicking NK/T cell lymphoma pathologically and benign features mimicking IM clinically. The findings indicated that AEBV + CT/NK-LH should be taken into consideration as a potential diagnosis, thus further clinical information (such as age of onset [whether children and young people], nature of onset [whether sudden], disease course [whether short], symptoms [whether systemic], EBV infection status [whether acute], and lymph node involvement) is needed for accurate diagnosis and prognostic evaluation.
WITHDRAWN: Is Replacement of Aromatic Compounds with Alkyl Ones Really the Effective Way toward Developing Greener Lubricants?
Authors: Wang Ying , Dong Rui , Wang Hanwen , Ma Lin , Fan Mingjin 
Published: February 2023

Abstract
The present work designs a series of cycloalkyl ester oils and reports a comparative study on their physicochemical and tribological properties, biological toxicity and degradability. From the perspective of molecular structure, the factors affecting their physicochemical, tribological properties, biological toxicity and degradability were discussed, including steric hindrance, group polarity and so on. The results demonstrated that cycloalkyl ester oils had better thermal stability and viscidity-temperature capability than the reference oils. Additionally, their friction reducing and anti-wear performance was superior to the reference samples. Unexpectedly, the cycloalkyl ester oils were found to have higher toxicity to the experimental plants (wheat and pak choi) due to the introducing of the cycloalkyl group, which enhanced the lipophilicity of the corresponding oils and then strengthen the damaging of the oils to plant cell. Some of the cycloalkyl ester oils, on the other hand, were proved to be more biodegradable than the reference samples. On the whole, developing green lubricants by replacing aromatic rings is not entirely successful. Lubricants' biodegradability can be improved with this replacement, but their ecotoxicity cannot be reduced. Anyway, the present work provides a specific benchmark for the subsequent development of environmentally friendly, high-performance synthetic ester lubricants.
Division of labor and cooperation between different butyrophilin proteins controls phosphoantigen-mediated activation of human γδ T cells
Authors: Karunakaran Mohindar Murugesh , Subramanian Hariharan , Jin Yiming , Mohammed Fiyaz , Kimmel Brigitte , Juraske Claudia , Starick Lisa , Nöhren Anna , Länder Nora , Willcox Carrie R. , Singh Rohit , Schamel Wolfgang W. , Nikolaev Viacheslav O. , Kunzmann Volker , Wiemer Andrew J. , Willcox Benjamin E. , Herrmann Thomas 
Published: February 2023

Abstract
Butyrophilin (BTN)-3A and BTN2A1 molecules control TCR-mediated activation of human Vγ9Vδ2 T-cells triggered by phosphoantigens (PAg) from microbes and tumors, but the molecular rules governing antigen sensing are unknown. Here we establish three mechanistic principles of PAg-action. Firstly, in humans, following PAg binding to the BTN3A1-B30.2 domain, Vγ9Vδ2 TCR triggering involves the V-domain of BTN3A2/BTN3A3. Moreover, PAg/B30.2 interaction, and the critical γδ-T-cell-activating V-domain, localize to different molecules. Secondly, this distinct topology as well as intracellular trafficking and conformation of BTN3A heteromers or ancestral-like BTN3A homomers are controlled by molecular interactions of the BTN3 juxtamembrane region. Finally, the ability of PAg not simply to bind BTN3A-B30.2, but to promote its subsequent interaction with the BTN2A1-B30.2 domain, is essential for T-cell activation. Defining these determinants of cooperation and division of labor in BTN proteins deepens understanding of PAg sensing and elucidates a mode of action potentially applicable to other BTN/BTNL family members.