bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oil spills, coastal pollution, and antibiotic resistance in bacteria isolated from soil across the Arabian Sea and Bay of Bengal coastlines
Authors: Rekadwad Bhagwan Narayan , Shouche Yogesh S. , Jangid Kamlesh 
Published: July 2023

Abstract
Pelagic transport causes oil pollution by traversing the open ocean in southern Asia and Indian territory via international tanker routes. We report that the traffic of sailing flags across oceans causes pollution in the Bay of Bengal and the Arabian Sea, which results in the formation of harmful algal blooms and an increase in chlorophyll productivity. The natural flow of ocean water from east to west, which carries pollutants into the marine waters belonging to the Indian territory, causes the deposition and saturation of oil spills and biohazardous wastes. The consequences of pollution have mostly affected coastal areas and caused the mortality of fauna. Metagenome analysis indicates a reduced microbial load and the presence of fewer microbial features across coastal soil. Long-term exposure to pollutants results in an unusual increase in cell size and the development of hydrocarbon- and antibiotic-resistant profiles. Bacterial taxa such as Brevundimonas, Streptococcus, Mycolibacterium, Sphingomonas, Bacillus spp., Chitinophaga, Priestia, Domibacillus, Rossellomorea, Acinetobacter towneri, etc., were found, and their identities were confirmed by 16S rRNA gene sequencing. These bacteria pose a serious threat to public health.
Research on nonlinear static and dynamic characteristics of linear guideway considering carriage flexibility
Authors: Liu Chang , Zhao Chunyu , Li Haolin 
Published: July 2023

Abstract
This paper establishes a five degree-of-freedom nonlinear dynamic model of the linear guideway considering the carriage flexibility, which is used to analyze the ball-groove load distribution and nonlinear dynamic characteristics under the action of the complex external loads. Firstly, according to the coordinate transformation matrix, the position relation between the groove centers when the carriage is a rigid body is determined. Next, the FE simulation is used to calculate the carriage flexible deformation caused by the contact loads, and the relation curves between the contact load and the displacement of the carriage groove center are determined. Then, the final position of the groove centers considering the carriage flexibility is determined, a 5 degree-of-freedom nonlinear dynamic model of the linear guideway is established. Finally, considering the possibility of the ball-groove losing contact, the Newmark-Newton numerical algorithm is established to solve the proposed dynamic model, and the proposed model is validated through experiments. Furthermore, the nonlinear static and dynamic characteristics of the linear guideway under the effect of the carriage flexibility are studied, and the effects of the design parameters on the vibration characteristics are discussed.This paper establishes a five degree-of-freedom nonlinear dynamic model of the linear guideway considering the carriage flexibility, which is used to analyze the ball-groove load distribution and nonlinear dynamic characteristics under the action of the complex external loads. Firstly, according to the coordinate transformation matrix, the position relation between the groove centers when the carriage is a rigid body is determined. Next, the FE simulation is used to calculate the carriage flexible deformation caused by the contact loads, and the relation curves between the contact load and the displacement of the carriage groove center are determined. Then, the final position of the groove centers considering the carriage flexibility is determined, a 5 degree-of-freedom nonlinear dynamic model of the linear guideway is established. Finally, considering the possibility of the ball-groove losing contact, the Newmark-Newton numerical algorithm is established to solve the proposed dynamic model, and the proposed model is validated through experiments. Furthermore, the nonlinear static and dynamic characteristics of the linear guideway under the effect of the carriage flexibility are studied, and the effects of the design parameters on the vibration characteristics are discussed.
Native top-down proteomics reveals EGFR–ERα signaling crosstalk in breast cancer cells dissociates NUTF2 dimers to modulate ERα signaling and cell growth
Authors: III John Yates , Gomes Fabio , Durbin Kenneth , Schauer Kevin , Nwachukwu Jerome , Russo Robin , Njeri Jacqline , Saviola Anthony , McClatchy Daniel , Diedrich Jolene , Garrett Patrick , Papa Alexandra , Ciolacu Ianis , Kelleher Neil , Nettles Kendall 
Published: July 2023

Abstract
Oligomerization of proteins and their modified forms (proteoforms) produces functional protein complexes 1,2. Complexoforms are complexes that consist of the same set of proteins with different proteoforms 3. The ability to characterize these assemblies within cells is critical to understanding the molecular mechanisms involved in disease and to designing effective drugs. An outstanding biological question is how proteoforms drive function and oligomerization of complexoforms. However, tools to define endogenous proteoform-proteoform/ligand interactions are scarce 4. Here, we present a native top-down proteomics (nTDP) strategy that combines size-exclusion chromatography, nano liquid-chromatography in direct infusion mode, field asymmetric ion mobility spectrometry, and multistage mass spectrometry to identify protein assemblies (≤70 kDa) in breast cancer cells and in cells that overexpress EGFR, a resistance model of estrogen receptor-α (ER-α) targeted therapies. By identifying ~104 complexoforms from 17 protein complexes, our nTDP approach revealed several molecular features of the breast cancer proteome, including EGFR-induced dissociation of nuclear transport factor 2 (NUTF2) assemblies that modulate ER activity. Our findings show that the K4 and K55 posttranslational modification sites discovered with nTDP differentially impact the effects of NUTF2 on the inhibition of the ER signaling pathway. By characterizing endogenous proteoform-proteoform/ligand interactions, we reveal the molecular diversity of complexoforms, which allows us to propose a model for ER drug discovery in the context of designing effective inhibitors to selectively bind and disrupt the actions of targeted ER complexoforms.
miR-128-3p inhibits intramuscular adipocyte differentiation in chicken by down regulating FDPS
Authors: Zhu Shuaipeng , Zhang Binbin , Zhu Tingqi , Wang Dongxue , Liu Cong , Liu Yixuan , He Yuehua , Liang Wenjie , Li Wenting , Han Ruili , li Donghua , Yan Fengbin , Tian Yadong , Li Guoxi , Kang Xiangtao , Li Zhuanjian , Jiang Ruirui , Sun Guirong 
Published: July 2023

Abstract
Background
Intramuscular fat (IMF) content is the major evaluating indicator in chicken meat quality. We previously found that miR-128-3p was differentially expressed in chicken intramuscular adipocyte at different periods. However, little is known about miR-128-3p associated with intramuscular adipocyte differentiation. The purpose of this present study was to investigate the biological functions and regulatory mechanism of miR-128-3p in chicken intramuscular adipogenesis.
Results
A series of assay results confirmed that miR-128-3p could promote the proliferation of intramuscular adipocytes and inhibit cell differentiation. A total of 223 and 1,050 differentially expressed genes (DEGs) were identified in the mimic treatment group and inhibitor treatment group compared with the control group, respectively. Functional enrichment analysis revealed that DEGs involved in lipid metabolism related processes, such as MAPK signaling pathway and TGF-β signaling pathway. Furthermore, target genes prediction analysis showed that miR-128-3p can target multiple genes from DEGs, such as FDPS, GGT5, TMEM37, and ASL2. Luciferase assay showed that the miR-128-3p targeted to the 3’ UTR of FDPS. Subsequent functional assays demonstrated that miR-128-3p acted as an inhibitor of intramuscular adipocyte differentiation by targeting FDPS.
Conclusion
This study found that miR-128-3p inhibited chicken intramuscular adipocytes differentiation by down regulating FDPS. Our findings provided a theoretical basis for the study of lipid metabolism, and also provided a potential target for molecular breeding to improve meat quality.
Effect of resilience training on stress, hope and psychological toughness of mothers with mentally and physically disabled children
Authors: Sharifian Pegah , kuchaki Zeinab , shoghi Mahnaz 
Published: July 2023

Abstract
Introduction:
The present study aimed at determining the impact of resilience training on stress, hope and psychological toughness of mothers with mentally and physically disabled children.
Materials and methods
In this interventional study, 70 parents of children with mental and physical disabilities were selected in an accessible manner and then classified into two control and intervention groups by random block allocation. In the intervention group, nine endurance training sessions were held for 60 min, one session per week at the welfare and rehabilitation center for mothers. Immediately before and one month after the intervention, the parenting stress, hope and psychological toughness questionnaire was completed by the mothers. The data were analyzed using Chi-square, Kruskal-Wallis, Fisher's exact test and t-test by SPSS software (version 23) at a significance level of 0.05.
Results
There was no significant difference in the parental stress between before and after the intervention in the both intervention and control groups (0.370), but it was significant after the intervention (0.001). Moreover, no significant difference was observed in parental hope before the intervention (0.452), while it was significant after the intervention (0.001). Parental psychological toughness was not significant before the intervention (0.179), while it was significant after the intervention (0.000).
Conclusion
Based on the results, resilience training reduced parental stress and increased hope and tenacity in mothers of the test group. Therefore, resilience training is recommended to reduce parental stress and increased hope and psychological toughness of mothers of mentally and physically disabled children.
JWST detection of heavy neutron capture elements in a compact object merger
Authors: Levan Andrew , Gompertz Benjamin , Salafia Om Sharan , Bulla Mattia , Burns Eric , Hotokezaka Kenta , Izzo Luca , Lamb Gavin , Malesani Daniele , Oates Samantha , Ravasio Maria , Escorial Alicia Rouco , Schneider Benjamin , Sarin Nikhil , Schulze Steve , Tanvir Nial , Ackley Kendall , Anderson Gemma , Brammer Gabriel , Christensen Lise , Dhillon Vikram , Evans Phil , Fausnaugh Michael , Fong Wen-fai , Fruchter Andrew , Fryer Chris , Fynbo Johan , Gaspari Nicola , Heintz Kasper , Hjorth Jens , Kennea Jamie , Kennedy Mark , Laskar Tanmoy , Leloudas Giorgos , Mandel Ilya , Martín-Carrillo Antonio , Metzger Brian , Nicholl Matt , Nugent Anya , Palmerio Jesse , Pugilese Giovanna , Rastinejad Jillian , Rhodes Lauren , Rossi Andrea , Smartt Stephen , Stevance Heloise , Tohuvavohu Aaron , Horst Alexander van der , Vergani Susanna , Watson Darach , Barclay Thomas , Bhirombhakdi Kornpob , Breedt Elme , Breeveld Alice , Brown Alex , Campana Sergio , D'Avanzo Paolo , D'Elia Valerio , De Pasquale Massimiliano , Dyer Martin , Galloway Duncan , Garbutt James , Green Matthew , Hartmann Dieter , Jakobsson Pall , Kerry Paul , Langeroodi Danial , Leung James , Littlefair Stuart , Munday James , O'Brien Paul , Parsons Steven , Pelisoli Ingrid , Sahman Dave , Salvaterra Ruben , Tagliaferri Gianpiero , Thöne Christina , Postigo Antonio de Ugarte , Sbarufatti Boris , Chrimes Ashley , Steeghs Danny , Kann David 
Published: July 2023

Abstract
The mergers of binary compact objects such as neutron stars and black holes are of central interest to several areas of astrophysics, including as the progenitors of gamma-ray bursts (GRBs), sources of high-frequency gravitational waves and likely production sites for heavy element nucleosynthesis via rapid neutron capture (the r-process). These heavy elements include some of great geophysical, biological and cultural importance, such as thorium, iodine, and gold. Here we present observations of the exceptionally bright gamma-ray burst GRB230307A. We show that GRB 230307A belongs to the class of long-duration gamma-ray bursts associated with compact object mergers, and contains a kilonova similar to AT2017gfo, associated with the gravitational-wave merger GW170817. We obtained James Webb Space Telescope mid-infrared (mid-IR) imaging and spectroscopy 29 & 61 days after the burst. The spectroscopy shows an emission line at 2.1 microns which we interpret as tellurium (atomic mass A=130), and a very red source, emitting most of its light in the mid-IR due to the production of lanthanides. These observations demonstrate that nucleosynthesis in GRBs can create r-process elements across a broad atomic mass range and play a central role in heavy element nucleosynthesis across the Universe.
Cancer cells transfer invasive properties through collagen-tracks
Authors: Saltel Frederic , Normand Léa , Rouyer Lucile , Richard Elodie , Allain Nathalie , Di-Tommaso Sylvaine , Dourthe Cyril , Anne-Aurélie Raymond , Dupuy Jean-Wiliam , Moreau Violaine , McGrogan Gaetan , Dugot-Senant Nathalie , Bouter Anthony , Tan Sisareuth , Favereaux Alexandre , Ros Manon 
Published: July 2023

Abstract
Invasion and migration through the extracellular matrix are prerequisites for metastasis, the leading cause of cancer-related deaths. During tumor development, the extracellular matrix is remodeled, including by overexpressing type I collagen that facilitate cancer dissemination. Most studies have focused on events at the leading edge as cells invade. We describe a new event at the trailing edge, as cells detach from the matrix.
We show that small vesicles containing the collagen receptor DDR1 are left behind on collagen fibrils in the migration path. We named these structures attached to the collagen fibers "collagen-tracks". The vesicles are similar in size to exosomes but lack the exosome markers, and they also are different to migrasomes. We show that collagen-track formation is stimulated by DDR1 and by factors that promote adhesion, including collagen cross-linking. We report the protein, mRNA and miRNA content of collagen-tracks. They contain adhesion proteins, suggesting that they form when membrane fragments containing adhesions are torn from the cell as it migrates along collagen fibrils. We show that collagen-tracks are deposited by breast cancer cells in 3D matrices in vitro and in vivo. Collagen-tracks are stable and can be taken up by surrounding cells, promoting epithelial to mesenchymal transition, matrix degradation and invasion, finally leading to increased lung metastasis of breast cancer cells.
In summary, we have identified and characterized a new vesicle entity directly attached to collagen fibrils that plays a role in cell-cell communication and can transfer invasive properties to surrounding cells. We conclude that cancer-related collagen-tracks are a new player acting locally to drive tumor invasion and metastasis
Preterm birth associated factors. A case-control study.
Authors: Almaghaslah Eman , Saif Ahmed Al , Algadeeb Kumail , Albannay Zainab , AlThani Jassem , Al-Saleh Wardah , Alrashed Malak , AlSaleh Hanin 
Published: July 2023

Abstract
Background: Preterm births are an increasing trend globally, and it demands healthcare and social support. Thus, studying the sociodemographic risk factors associated with preterm birth reflects a better understanding and enables the development of efficient, cost-effective maternal health services.
Objective: To assess the association between pregnancy planning and preterm birth. Then, our secondary objective is to investigate the sociodemographic factors among preterm and full-term delivered women.
Methods: A case‒control study was conducted on n=391 postpartum women. Fifty-two preterm births (cases) and 339 full-term births (controls) were performed in a referring hospital in Hofuf, Saudi Arabia. A survey is collected from postpartum women rating pregnancy planning practices and other sociodemographic characteristics.
Results: The majority, 85% of the sample, were housewives, and 80% were within a low-income category earning less than 10,000 SR per month for both preterm and full-term mothers. We found a greater odds of having chronic illness during pregnancy (OR= 1.76, 95% CI (1.062, 2.917)) the preterm birth mothers.
Conclusion and recommendation: Our study highlighted the preterm associated risk factors namely, presence of pregnancy illness, more likely to end by cesarian section, and to have a neonatal complication. Thus, the result highlighted the need for careful antenatal care pregnancy management for women with chronic illness such as diabetes, and hypertension. Therefore, we also recommend pregnancy preparation and planning for healthy pregnancy specially for high-risk pregnancy.
The arbitrary value of time as a key quality indicator for EMS care: An observational study using the SPART model on the relationship between time and clinical reasoning activities by EMS providers.
Authors: Dercksen Bert , Struys Michel.M.R.F. , Paans Wolter , Cnossen Fokie 
Published: July 2023

Abstract
Background
Time is a key quality indicator for EMS care, and many efforts are directed at minimizing the time used for response, on-site and transport periods. In some deployments, this is undoubtedly justified, but in many medical situations, time might be overestimated as a quality indicator. To assess the value of time as a quality indicator, we conceived a study to investigate the relationship between EMS clinical reasoning processes and the variable time.
Methods
Using video recordings of EMS deployments, we qualitatively identified professional activities, determined their time of execution within a deployment and measured the duration of these activities. The observed activities were classified using the SPART model, which identifies ten categories in the EMS care process. We assigned each diagnostic and treatment activity to a category. The measured time variables were interpreted both qualitatively and by using descriptive statistics.
Results
We identified 1683 activities (total duration 12h34m30s, 28 clinical cases, 20 participants). Diagnostic activities took 4.3 times more time than therapy. The diagnostic process sometimes continued after the clinical decision had been made, but this did not alter the decision taken.
Conclusion
Our observations showed that most of the time was spent on the diagnostic process leading to a clinical decision. Sometimes, this process was terminated too early, and the clinical decision seemed to be taken too soon. This phenomenon is known as premature closure (a hypothesis is considered final before all evidence is searched for and evaluated), possibly negatively affecting the quality of care. We speculate that this cognitive bias could be minimized if the deployment duration is considered less critical, an issue we feel warrants further research.
PhCYP724B1 positively regulates shoot branching
Authors: Dong Lili , Xu Xiaorong , Jiang Ziying , Yao Huanyu , Tian Wang , Deng Xinyi 
Published: July 2023

Abstract

Background: Petunia hybrida is an important ornamental plant, and branch regulation is of great significance in its cultivation.
Results: We isolated the homologous gene of CYP724B1 from Petunia × hybrida cv 'Mitchell Diploid', and named PhCYP724B1. Expression analysis revealed that PhCYP724B1 was expressed highest in buds, while lowest in flowers. The expression level of PhCYP724B1 was higher in the petunia variety with more branches, while lower in variety with fewer branches. Subcellular localization assays demonstrated that PhCYP724B1 protein was localized on the endoplasmic reticulum. Furthermore, overexpression of PhCYP724B1 in transgenic Arabidopsis and petunia plants both enhanced shoot branching. In contrast, RNAi of PhCYP724B1 in petunia resulted in a significantly inhibited shoot branching phenotype.
Conclusions: Taken together, the results denote new insights into the important role of PhCYP724B1 in regulating petunia shoot branching.
Response surface methodology for modeling of the critical electric field of a single drop subjected to different electric waveforms
Authors: Shahmoradi Zahra , Mousavi Seyed Hamed 
Published: July 2023

Abstract
Electro-coalescence has been an environmentally friendly technology for decades. However, electric field strength should not exceed a critical value (Ecrit) to inhibit droplets from disintegrating during coalescence. In this study, response surface methodology (RSM) with a D-optimal design was utilized to develop a model to achieve the maximum Ecrit of a single drop. Waveform, frequency, drop diameter and interfacial tension were statistically significant. Frequency change revealed Ecrit increases with a moderate slope for all waveforms. This was attributed to less degree of drop deformation due to shorter on-time intervals of pulsatile electric field and non-compliance of drop vibration with field frequency. Following the revelation of interaction between diameter and frequency, it was observed elevated frequencies have a significant impact on larger droplets, and the sensitivity of Ecrit to the diameter decreases with frequency. This suggests higher frequencies as a useful and fast controllable variable to compensate for the effect of droplet size distribution. Optimization suggested a minimum drop diameter and a maximum frequency that can be used as two important limits for the robust design of electro-coalescers. The best and worst results in all cases corresponded to Pulse 90 and 10 waveforms respectively.
Developmental molecular controls over arealization of descending cortical motor pathways
Authors: Jabaudon Denis , Abe Philipp , Lavalley Adrien , Morassut Ilaria , Klingler Esther , Santinha Antonio , Platt Randall 
Published: July 2023

Abstract

Layer 5 extratelencephalic (ET) neurons are a main class of neocortical projection neurons that predominate in the motor cortex and send their axon to the pons and spinal cord, and collaterals to the thalamus and multiple deep subcerebral structures. Precise connectivity of ET neurons is critical for fine motor control; they are central to loss of function upon spinal cord injury and specifically degenerate in select neurodegenerative disorders. ET neurons consist of several types of cells with distinct laminar and areal locations, molecular identities, connectivities, and functions. Within layer 5 of the cortex, two cardinal subtypes of ET neurons have been identified: “ET lower” neurons, which express Slco2a1 and project to distal targets including the spinal cord, and “ET upper“ neurons, which express Nprs1 or Hpgd and project more proximally to the pons and thalamus. Despite their critical function, how these neuronal subtypes emerge during development and acquire their area-specific distributions remains unaddressed. Here, using combinations of anatomical labeling, MAPseq mapping, and single-nucleus transcriptomics across developing cortical areas, we reveal that these two subtypes of ET neurons are present at birth along opposite antero-posterior cortical gradients. We first characterize area-specific developmental axonal dynamics of ET lower and ET upper neurons and find that the latter can emerge by pruning of subsets of ET lower neurons. We next identify area- and ET neuron type-specific developmental transcriptional programs to identify key target genes in vivo. Finally, we reprogram ET neuron area-specific connectivity from motor to visual by postnatal in vivo combinatorial knockout of three key type-specific transcription factors. Together, these findings delineate the functional transcriptional programs controlling ET neuron diversity across cortical areas and provide a molecular blueprint to investigate and direct the developmental emergence of corticospinal motor control.