bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMODELAN KOMPUTASIONAL UNTUK MENENTUKAN KEKUATAN STRUKTUR BANGUNAN
Authors: Faqih Nasyiin , Laksono Ersyad Aji 
Published: June 2023

Seiring berjalannya waktu, peningkatan jumlah penduduk mengharuskan penambahan fasilitas dan kualitas pada Rumah Sakit Islam Wonosobo. Manajemen Rumah Sakit yang merencanakan pembangunan gedung baru untuk mewujudkan hal tersebut.
Gedung mengacu pada persyaratan sesuai S N I 2847-2019, S N I 1726-2019, S N I 1727-2013, S N I 1729-2015. Data yang digunakan melingkupi data sondir tanah dan gambar rencana bangunan.
Perencanaan dimulai dari pengumpulan data, preliminary desain, peninjauan pembebanan, permodelan struktur menggunakan aplikasi SAP2000 v.18, ETAB dan SPColoumn untuk mendapatkan gaya dalam. Data diolah untuk mengetahui kebutuhan tulangan dan diteruskan membuat gambar kerja menggunakan aplikasi Autocad 2013.
Hasil dari pengolahan data, bangunan menggunakan kolom 70x70cm, Balok B1 60x40cm, Balok B2 50x35cm, Balok anak 40x25cm, tebal pelat S1 15cm, pelat S2 12cm, pelat tangga dan bordes 20cm. Struktur bawah menggunakan pondasi dalam, terdiri dari pilecap 1 ukuran 210x210cm ditopang 4 boredpile berdiameter 50cm, pilecap 2 berukuran 250x120cm ditopang 2 boredpile berdiameter 50cm, kedalaman pondasi 7,80m.
Harapan untuk perencanaan gedung selanjutnya memahami dasar statistika dalam pengolahan data dari aplikasi yang digunakan. Serta menyiapkan dan mempelajari referensi yang digunakan guna hasil terbaik.
Prevalence of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, and Trichomonas vaginalis among patients suspected of sexually transmitted infections in Evercare Hospital Dhaka during 2015 to 2022
Authors: Hasan Nazmul , Rahim Rummana , Hasan Abu , Ali Rubaiya , Ullah QM Mahbub , Rahman Mizanur 
Published: June 2023

Background Sexually Transmitted Infections (STIs) are often diagnosed by clinical symptoms and signs due to lack of sensitive laboratory methods. Most of the time pathogens are not identified and antibiotics are used empirically. The purpose of our study is to find out the STI pathogens detected by sensitive multiplex PCR done routinely in a tertiary care hospital.
Materials and methods A total of 578 samples were tested from February 2015 to July 2022. Urine, prostatic secretion, urethral swab, high vaginal swab, semen, throat swabs were collected from symptomatic patients for the routine test. DNA was extracted and Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Trichomonas vaginalis were screened by using CE-IVD approved multiplex Real Time PCR kit.
Result Out of 578 cases, 76 (13.15%) were positive for at least one pathogen and 5 were co-infections. Out of 76 positive cases, Chlamydia trachomatis was found 41 (53.9%), Neisseria gonorrhoeae was 13 (17.1%), Mycoplasma genitalium was 12 (15.8%), and Trichomonas vaginalis 5 (6.6%) and co-infections with Neisseria gonorrhoeae and Chlamydia trachomatis was 5 (6.6%). Positivity rate was remarkably higher in male (77.6%) than female (22.4%) and predominant age group was 19-40 years.
Conclusion The predominant STIs pathogen found in our cohort is Chlamydia trachomatis followed by Neisseria gonorrhoeae. Multiplex PCR is a wide-ranging diagnostic method for the detection of multiple pathogens simultaneously which allows early and accurate diagnosis of STIs.
Pulse Volume 12-14 2020-2022 p.34-39
A Kaleidoscope of Play and Learning in Online Spaces during COVID-19 Times: Insights from Early Childhood Educators
Authors: Bonello Charmaine , Deguara Josephine , Milton Josephine , Camilleri Rosienne , Muscat Tania 
Published: June 2023

Through the perspectives of early childhood educators (ECEs) working with under-fives, this article explores how play and learning featured when shifted abruptly to online spaces during COVID-19 in Malta. This work draws on Siemens’ connectivism learning theory which sees the integration of technology and social interactions as connections that empower learning in a digital age. The COVID-19 scenario called for immediately available data; consequently, a quantitative methodology was adopted. Two online surveys were held: in 2020 and 2021. Two hundred sixty-three ECEs participated in the first online survey, while seventy-nine the second. Findings locate benefits, challenges, and opportunities for play and learning online, shaped by the development of three key changing patterns in Maltese ECEC: an uneven start for ECEs, e-interactions, and curricula and pedagogies in online spaces – a kaleidoscope of play and learning in COVID-19 times. Implications for pre-service and in-service ECEs’ support, monitoring, and training are discussed to facilitate effective play and learning in online environments.
A study to characterize the mechanical properties and material constitution of adult descending thoracic aorta based on uniaxial tensile test and digital image correlation
Authors: Li Zhengdong , Pei Ming , Zhang Jianhua , Liu Ningguo , Wang Jinming , Zou Donghua 
Published: June 2023

The mechanical properties and material constitution of the aorta are important in forensic science and clinical medicine. Existing studies on the material constitution of the aorta do not satisfy the practical requirements of forensic and clinical medicine, as the reported failure stress and failure strain values for human aortic materials have a high dispersion. In this study, descending thoracic aortas were obtained from 50 cadavers (dead within 24 h) free of thoracic aortic disease, aged from 27 to 86 years old, which were divided into six age groups. The descending thoracic aorta was divided into proximal and distal segments. A customized 4-mm cutter was used to punch a circumferential and an axial dog-bone-shaped specimen from each segment; the aortic ostia and calcification were avoided. Instron 8,874 and digital image correlation were used to perform a uniaxial tensile test on each sample. Four samples from each descending thoracic aorta produced ideal stress-strain curves. All parameter-fitting regressions from the selected mathematical model converged, and the best-fit parameters of each sample were obtained. The elastic modulus of collagen fiber, failure stress, and the strain showed a decreasing trend with age, while the elastic modulus of elastic fiber showed an increasing trend with age. The elastic modulus of collagen fiber, failure stress, and strain of circumferential tensile were all greater than those for axial tensile. There was no statistical difference in model parameters and physiological moduli between the proximal and distal segments. The failure stress and strain in the proximal circumferential, distal circumferential, and distal axial tensile were all greater for the male group than for the female group. Finally, the Fung-type hyperelastic constitutive equations were fitted for the different segments in different age groups.
Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity
Authors: Vámos Attila , Arianti Rini , Vinnai Boglárka Ágnes , Alrifai Rahaf , Shaw Abhirup , Póliska Szilárd , Guba Andrea , Csősz Éva , Csomós István , Mocsár Gábor , Lányi Cecilia , Balajthy Zoltán , Fésüs László , Kristóf Endre 
Published: June 2023

Introduction: White adipocytes store lipids, have a large lipid droplet and few mitochondria. Brown and beige adipocytes, which produce heat, are characterized by high expression of uncoupling protein (UCP) 1, multilocular lipid droplets, and large amounts of mitochondria. The rs1421085 T-to-C single-nucleotide polymorphism (SNP) of the human FTO gene interrupts a conserved motif for ARID5B repressor, resulting in adipocyte type shift from beige to white.Methods: We obtained abdominal subcutaneous adipose tissue from donors carrying FTO rs1421085 TT (risk-free) or CC (obesity-risk) genotypes, isolated and differentiated their preadipocytes into beige adipocytes (driven by the PPARγ agonist rosiglitazone for 14 days), and activated them with dibutyryl-cAMP for 4 hours. Then, either the same culture conditions were applied for additional 14 days (active beige adipocytes) or it was replaced by a white differentiation medium (inactive beige adipocytes). White adipocytes were differentiated by their medium for 28 days.Results and Discussion: RNA-sequencing was performed to investigate the gene expression pattern of adipocytes carrying different FTO alleles and found that active beige adipocytes had higher brown adipocyte content and browning capacity compared to white or inactive beige ones when the cells were obtained from risk-free TT but not from obesity-risk CC genotype carriers. Active beige adipocytes carrying FTO CC had lower thermogenic gene (e.g., UCP1, PM20D1, CIDEA) expression and thermogenesis measured by proton leak respiration as compared to TT carriers. In addition, active beige adipocytes with CC alleles exerted lower expression of ASC-1 neutral amino acid transporter (encoded by SLC7A10) and less consumption of Ala, Ser, Cys, and Gly as compared to risk-free carriers. We did not observe any influence of the FTO rs1421085 SNP on white and inactive beige adipocytes highlighting its exclusive and critical effect when adipocytes were activated for thermogenesis.
The fecal carriage rate of extended-spectrum β-lactamase–producing or carbapenem-resistant Enterobacterales among Japanese infants in the community at the 4-month health examination in a rural city
Authors: Kawata Soichiro , Morimoto Shimpei , Kosai Kosuke , Kawamoto Yasuhide , Nakashima Yumiko , Morinaga Yoshitomo , Yanagihara Katsunori , Yoshida Lay-Myint , Moriuchi Hiroyuki 
Published: June 2023

BackgroundExtended-spectrum β-lactamase–producing Enterobacterales (ESBL-E) is a great public health concern globally not only in hospitals but also in the community. To our knowledge, there have been few studies on the prevalence of ESBL-E and much less about carbapenem-resistant Enterobacterales (CRE) among children in the community, and there is no such study in Japan despite such situations. This study aimed to clarify their carriage status among Japanese infants in the community by taking the opportunity of the 4-month health checkup.MethodsThis prospective analysis was conducted from April 2020 to March 2021 in Shimabara City, Nagasaki Prefecture, Japan. The research-related items were mailed to all subjects with official documents for the checkup. The fecal samples were obtained from the diaper by guardians beforehand and were collected with the questionnaire and then screened for ESBL-E and CRE by a clinical laboratory company with selective agars followed by identification and confirmation. Only the positive samples were analyzed about resistant genotypes.ResultsOne hundred fifty infants aged 4–5 months, over half of the subjects, participated in this study. The overall ESBL-E carriage rate was 19.3% (n = 29), and no CRE carrier was detected among them. All identified ESBL-E were E. coli except for one K. pneumoniae. A significantly higher carriage rate was recorded among the infants born at “Hospital A” (25.0%) than the others (11.3%). Enterobacterales producing CTX-M-9 ± TEM were broadly distributed among the positive samples (65.5%), whereas the CTX-M-1 group was exclusively detected among those from “Hospital A”. Recursive partitioning analysis suggested that delivery facilities might be an important factor for ESBL-E colonization, although the effect could be decreased as they grow. In contrast, no significant effect was observed for other factors such as parent(s) as healthcare worker(s), having a sibling(s), and the mode of delivery.ConclusionThis study revealed the ESBL-E and CRE carriage status of Japanese infants in the community for the first time, although the setting is somewhat limited. Our findings indicated that environmental factors, especially delivery facilities, influenced ESBL-E colonization among infants aged 4–5 months, implying the need for strengthening countermeasures against antimicrobial resistance at delivery facilities and communities outside the hospitals.
The outer membrane proteins based seroprevalence strategy for Brucella ovis natural infection in sheep
Authors: Zhang Tao , Wang Yu , Li Yin , Qi Tingting , Yue Zhirong , Cao Lili , Zhou Bo , Jiao Huping 
Published: June 2023

IntroductionThe diagnosis of brucellosis largely relies on tiger red plate agglutination test (RBPT). However, it is difficult to distinguish between natural infection antibody positive and vaccination antibody positive, nevertheless, the identification of specific Brucella species natural infection.MethodsHere, we analyzed the structure of main outer membrane proteins (OMPs), OMP25 and OMP31 from Brucella ovis (B. ovis) and Brucella melitensis (B. melitensis), which are the main pathogens of sheep brucellosis, and found the OMP25 and OMP31 could be used as the differential antigens for B. ovis and B. melitensis antibody. Then we expressed the OMP25 from B. ovis (OMP25o) and OMP31 from B. melitensis (OMP31m).ResultsThey have equally efficiency in antibody detection of vaccinated sheep serum, consistent with the RBPT results. However, through epidemiological investigations, we found some RBPT positive samples were negative by the OMP31m based serum antibody detection, but these samples gave positive results by the OMP25o. We verified these OMP31m negative but OMP25o positive samples by B. ovis and B. melitensis specific primers based PCR detection, and all these samples were B. melitensis negative. However, four out of six samples are B. ovis positive. These results showed that we could use the OMP25o and OMP31m to diagnose sheep brucellosis antibody, especially to discriminate the infection of the B. ovis.DiscussionCurrently, China has not yet approved a vaccine based on B. ovis and B. ovis positive samples should be naturally infected. There should be some implicit transmission of B. ovis in Jilin province. Further epidemiological investigation should be conducted to monitor the B. ovis natural infection.
Long-term outcomes of drug-coated balloon treatment of calcified coronary artery lesions: a multicenter, retrospective, propensity matching study
Authors: Shan Yingguang , Lu Wenjie , Han Zhanying , Pan Sancong , Li Xiangbing , Wang Xi , Pan Liang , Wang Xule , Zheng Xiaolin , Li Ran , Zhou Yanjun , Qin Peng , Shi Qiangwei , Zhou Shuai , Zhang Wencai , Guo Sen , Qiu Jing , Zhang Peisheng , Qin Xiaofei , Sun Guoju , Qin Zhongsheng , Huang Zhenwen , Qiu Chunguang 
Published: June 2023

BackgroundCoronary artery calcification (CAC) is associated with high rates of restenosis and adverse clinical events after percutaneous coronary intervention (PCI) with drug-eluting stents (DES).ObjectivesThe aim of this study was to evaluate the long-term clinical outcomes of drug-coated balloon (DCB)-only treatment for de novo lesions with and without CAC.MethodsPatients with de novo coronary disease treated with the DCB-only strategy were retrospectively enrolled from three centers and categorized into a CAC group and a non-CAC group. The primary endpoint was the target lesion failure (TLF) rate during the 3-year follow-up. Secondary endpoints included the occurrence of major adverse cardiac events (MACEs), target lesion revascularization (TLR), cardiac death, myocardial infarction (MI) and any revascularization. Propensity score matching (PSM) was conducted to assemble a cohort of patients with similar baseline characteristics.ResultsA total of 1,263 patients with 1,392 lesions were included, and 243 patients were included in each group after PSM. Compared with the non-CAC group, the incidence rates of TLF (9.52% vs. 4.94%, odds ratio [OR]: 2.080; 95% confidence interval [CI]: 1.083–3.998, P = 0.034) and TLR (7.41% vs. 2.88%, OR: 2.642; 95% CI: 1.206–5.787, P = 0.020) in the CAC group were higher. The incidence rates of MACE (12.35% vs. 7.82%, OR: 1.665; 95% CI: 0.951–2.916, P = 0.079), cardiac death (2.06% vs. 2.06%, OR: 0.995; 95% CI: 0.288–3.436, P = 0.993), MI (1.23% vs. 0.82%, OR: 2.505; 95% CI: 0.261–8.689, P = 0.652) and any revascularization (12.76% vs. 9.67%, OR: 1.256; 95% CI: 0.747–2.111, P = 0.738) were similar between groups.ConclusionsCAC increased the incidence of TLF and TLR without a substantial increase in the risk of MACE, cardiac death, MI, or any revascularization in patients treated with DCB-only angioplasty during the 3-year follow-up.
Non-contrast transcatheter aortic valve implantation for patients with aortic stenosis and chronic kidney disease: a pilot study
Authors: Freire Antônio Fernando Diniz , Nicz Pedro Felipe Gomes , Ribeiro Henrique Barbosa , Filippini Filippe Barcellos , Accorsi Tarso Duenas , Liberato Gabriela , Nomura Cesar Higa , Cassar Renata de Sa , Vieira Marcelo Luiz Campos , Mathias Wilson , Pomerantzeff Pablo Maria Alberto , Tarasoutchi Flavio , Abizaid Alexandre , Kalil Filho Roberto , Brito Fábio Sândoli de 
Published: June 2023

BackgroundAcute kidney injury (AKI) is frequently observed after transcatheter aortic valve implantation (TAVI). Of note, it is associated with a threefold increase in all-cause and cardiac death. We propose a new non-contrast strategy for evaluating and performing the TAVI procedure that can be especially valuable for patients with aortic stenosis (AS) and chronic kidney disease (CKD) to prevent AKI.MethodsPatients with severe symptomatic AS and CKD stage ≥3a were evaluated for TAVI using four non-contrast imaging modalities for procedural planning: transesophageal echocardiogram (TEE), cardiac magnetic resonance, multidetector computed tomography (MDCT), and aortoiliac CO2 angiography. Patients underwent transfemoral (TF) TAVI using the self-expandable Evolut R/Pro, and the procedures were guided by fluoroscopy and TEE. Contrast MDCT and contrast injection at certain checkpoints during the procedure were used in a blinded fashion to guarantee patient safety.ResultsA total of 25 patients underwent TF-TAVI with the zero-contrast technique. The mean age was 79.9 ± 6.1 years, 72% in NYHA class III/IV, with a mean STS-PROM of 3.0% ± 1.5%, and creatinine clearance of 49 ± 7 ml/min. The self-expandable Evolut R and Pro were implanted in 80% and 20% of patients, respectively. In 36% of the cases, the transcatheter heart valve (THV) chosen was one size larger than the one by contrast MDCT, but none of these cases presented adverse events. Device success and the combined safety endpoint (at 30 days) both achieved 92%. Pacemaker implantation was needed in 17%.ConclusionThis pilot study demonstrated that the zero-contrast technique for procedural planning and THV implantation was feasible and safe and might become the preferable strategy for a significant population of CKD patients undergoing TAVR. Future studies with a larger number of patients are still needed to confirm such interesting findings.
The influence of the Pringle maneuver in laparoscopic hepatectomy: continuous monitor of hemodynamic change can predict the perioperatively physiological reservation
Authors: Chen Yi-Chan , Lee Min-Hsuan , Hsueh Shan-Ni , Liu Chien-Liang , Hui Chung-Kun , Soong Ruey-Shyang 
Published: June 2023

ImportanceThis is the first study to investigate the correlation between intra-operative hemodynamic changes and postoperative physiological status.ObjectiveDesign, settings, and participantsPatients receiving laparoscopic hepatectomy were routinely monitored using FloTract for goal-directed fluid management. The Pringle maneuver was routinely performed during parenchymal dissection and the hemodynamic changes were prospectively recorded. We retrospectively analyzed the continuous hemodynamic data from FloTrac to compare with postoperative physiological outcomes.ExposureThe Pringle maneuver during laparoscopic hepatectomy.Main outcome(s) and measure(s)ResultsStroke volume variation that did not recover from the relief of the Pringle maneuver during the last application of Pringle maneuver predicted elevated postoperative MELD-Na scores.Conclusions and relevanceThe complexity of the hemodynamic data recorded by the FloTrac system during the Pringle Maneuver in laparoscopic hepatectomy can be effectively analyzed using the growth mixture modeling (GMM) method. The results can potentially predict the risk of short-term liver function deterioration.
Evaluation and engineering applications of the in situ stress state of deep tight sandstone reservoirs in the Xujiahe Formation of the Yingshan-Longgang in the central Sichuan Basin
Authors: Gao Songyang , Deng Hucheng , Yan Weilin , Yin Shujun , Zhou Jiayu , Wen Zheng , Guo Yunfei , Zhang Bo , Li Chuang , Yang Xiaolei , Xia Wenhao , Huang Tao 
Published: June 2023

Introduction: The Xujiahe Formation in the Yingshan-Longgang area of the Sichuan Basin has abundant tight oil and gas resources. However, the complex geological conditions and insufficient understanding of the magnitude and direction of in-situ stresses severely limit exploration and development of oil and gas resources in the region. This has made it difficult to carry out hydraulic fracturing and horizontal well deployment in the region.Methods: Based on experimental analyses, including acoustic emission experiments, wave velocity anisotropy experiments, and paleomagnetic experiments, this study evaluated the magnitude and direction of in-situ stresses, while also combining electrical imaging logging and acoustic wave logging data. This provides a basis for hydraulic fracturing in the research area.Results and Discussion: The experimental results indicate that the three-way stress state of the Xujiahe Formation is a strike-slip stress state, and the three-way stress value gradually increases with increasing burial depth. The Longgang area is particularly affected by the depth. The average azimuth angle of the maximum horizontal principal stress in the study area is N100°E, while tectonic deformation may cause a counterclockwise deviation of the maximum horizontal principal stress azimuth in the Yingshan area. Considering the influence of changes in the magnitude of in situ stress in the longitudinal direction on hydraulic fracturing extension mode, it is recommended to select the middle and upper sections of the Xu II for fracturing and transformation. Similarly, considering the distribution of natural fractures and the direction of the in situ stresses, it is suggested that the deployment azimuth angle for horizontal wells be between N20°–30°E.
Watchful waiting versus pharmacological management of small-for-gestational-age infants with hyperinsulinemic hypoglycemia
Authors: Chandran Suresh , Jaya-Bodestyne Sandra Lynn , Rajadurai Victor Samuel , Saffari Seyed Ehsan , Chua Mei Chien , Yap Fabian 
Published: June 2023

IntroductionGiven that reports on severe diazoxide (DZX) toxicity are increasing, we aimed to understand if the short-term clinical outcomes of small-for-gestational-age (SGA) infants with hyperinsulinemic hypoglycemia (HH) managed primarily by supportive care, termed watchful waiting (WW), are different from those treated with DZX.MethodA real-life observational cohort study was conducted from 1 September 2014 to 30 September 2020. The WW or DZX management decision was based on clinical and biochemical criteria. We compared central line duration (CLD), postnatal length of stay (LOS), and total intervention days (TIDs) among SGA-HH infants treated with DZX versus those on a WW approach. Fasting studies determined the resolution of HH.ResultAmong 71,836 live births, 11,493 were SGA, and 51 SGA infants had HH. There were 26 and 25 SGA-HH infants in the DZX and WW groups, respectively. Clinical and biochemical parameters were similar between groups. The median day of DZX initiation was day 10 of life (range 4–32), at a median dose of 4 mg/kg/day (range 3–10). All infants underwent fasting studies. Median CLD [DZX, 15 days (6–27) vs. WW, 14 days (5–31), P = 0.582] and postnatal LOS [DZX, 23 days (11–49) vs. WW, 22 days (8–61), P = 0.915] were comparable. Median TID was &gt;3-fold longer in the DZX than the WW group [62.5 days (9–198) vs. 16 days (6–27), P &lt; 0.001].ConclusionCLD and LOS are comparable between WW and DZX groups. Since fasting studies determine the resolution of HH, physicians should be aware that clinical intervention of DZX-treated SGA-HH patients extends beyond the initial LOS.