bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is there a species swarm paradox? Spatial segregation of related species in the understorey of a tropical plant community
Authors: Ferreira‐Santos Karina , Carrijo Tatiana T. , Torres‐Leite Filipe , Hollunder Renan K. , Dias André T. C. , Garbin Mário L. 
Published: December 2022

AbstractQuestionIndividual plants of some highly diverse angiosperm families occur in aggregated spatial patterns in tropical plant communities, which have been defined as species swarms. Although this spatial aggregation seems paradoxical, because related species should segregate in space owing to expected ecological similarities, it has yet to be tested. We assessed whether species of the family Rubiaceae segregate in space in the understorey of a tropical plant community.LocationAn Atlantic Forest fragment in southeast Brazil.MethodsWe surveyed all local aggregates of Rubiaceae species within an 18.75‐ha plot across a topographic gradient. Based on the species abundance distribution, we used the 10 most abundant species to model their spatial patterns using univariate and bivariate spline spatial correlations. Bubble graphs were used to show how these species were distributed across the gradient.ResultsA total of 12,258 individuals of 47 species were found distributed in 543 local aggregates. The 10 studied species corresponded to 86% of the total abundance and were aggregated in space, at distances from 0 to 10–300 m. Pairwise comparisons revealed a segregation pattern at scales ranging from 0 to 10–230 m. Local aggregates were strongly dominated by a single species, whereas many other species occurred at very low abundances.ConclusionThe studied species of Rubiaceae shared the understorey environment by occupying different portions of available space. Habitat preferences, negative interactions, dispersal limitation and priority effects may explain the observed spatial patterns. Swarms are not paradoxical because species of Rubiaceae use different microhabitats within the forest understorey.
L’image de la Chinoise « désexuée » sous le regard de Beauvoir : De la critique à l’idéalisation
Authors: Liu Yangyang 
Published: December 2022

RésuméDans La longue marche, essai sur la Chine (Gallimard, 1957), malgré son admiration pour le pays maoïste, Beauvoir aborde un phénomène social controversé qui est la « désexualisation » de la femme chinoise. La réception beauvoirienne, en cette matière, est assez particulière, car elle témoigne d’une rupture entre l’horizon d’attente et le présent. Or, ses convictions socialiste et féministe l’incitent à modifier sa vision, plus précisément, à créer un nouvel horizon basé sur de nouvelles données : une fusion de sa vision du passé et celle de l’actuelle. Cela faisant, la Chinoise est transformée, à un certain degré, en un paradigme de l’idéalisme socialiste et féministe.
The Gossiper's high and low: Investigating the impact of negative gossip about the supervisor on work engagement
Authors: Zhong Rui , Tang Pok Man , Lee Stephen H. 
Published: December 2022

AbstractAlthough negative gossip is ubiquitous in the workplace, we know little about how negatively gossiping about the supervisor—who occupies a higher hierarchical position in the organization—influences gossipers themselves. To address this question, we draw on the conservation of resources theory to account for the resource‐consuming and resource‐generating impact of negative gossip about the supervisor on gossipers’ work engagement. Findings from three experience sampling studies show that negative gossip about the supervisor is a double‐edged sword for gossipers that seems to do more harm than good to their work engagement. On the one hand, spreading negative gossip about the supervisor evokes the resource‐consuming mechanism of image maintenance concerns, which impairs gossipers’ work engagement, especially when perceived organizational politics is higher. On the other hand, engaging in such gossip elicits the resource‐generating mechanism of sense of power, which only improves work engagement in Study 3 but not in Studies 1 and 2; contrary to our expectation, this effect is unaffected by perceived organizational politics. We conclude by discussing the theoretical and practical contributions of our research.
Partners' internal stress and well‐being: The role of dyadic coping and problem resolution
Authors: Donato Silvia , Pagani Ariela F. , Rosta‐Filep Orsolya , Faggiano Manuel , Pajardi Daniela , Martos Tamas , Sallay Viola , Randall Ashley K. 
Published: December 2022

AbstractCouples' experiences of daily stress can be detrimental for partners' individual and relational well‐being, specifically their identity as a couple, their relational satisfaction, and their life satisfaction. Grounded in the Systemic Transactional Model, this study aimed at analyzing factors that may safeguard partners and their relationship from detrimental effects of internal stress (i.e., stress that originates inside the relationship). We examined the buffering effect of partners' positive dyadic coping and internal problem resolution. Daily diary data were collected across 7 days from 82 heterosexual couples. Multilevel dyadic analyses showed that internal stress was negatively associated with partners' individual and relational well‐being. Positive dyadic coping moderated the association between partners' internal stress and couple satisfaction for both partners, but not life satisfaction and couple identity. Moreover, for partners who reported a resolution to the internal problem, the negative associations of internal stress with life satisfaction, couple satisfaction, and couple identity were significantly lower than for those who did not resolve the internal problem. This study confirms the negative role of internal stress on well‐being, shows the associations between internal stress and couple identity, and highlights the protective role of dyadic coping and internal problem resolution in couples' daily lives.
Factors influencing loneliness among older people using homecare services during the COVID‐19 pandemic
Authors: Wang Yu‐Chi , Lin Pin‐Hsuan , Lin Ying‐Lien , Lee Ya‐Chen , Wang Jiun‐Yi , Chen Yi‐Ching , Yang Shang‐Yu 
Published: December 2022

AbstractBackgroundElderly homecare service users may reduce their level of social participation and interpersonal interactions due to physiological loss, which may lead to loneliness and depression over the years. However, there is a lack of research on loneliness among older people who use homecare services. The purpose of this study was to examine the factors influencing loneliness among older people using homecare services.MethodsThis is a longitudinal study conducted in communities in Central Taiwan, and data were collected using a structured questionnaire. The questionnaire was first administered as a pre‐test to obtain baseline information about the participants, and the same questionnaire was administered as a post‐test after 6 months to follow‐up. The pre‐ and post‐test questionnaires included five sections, that is, participant demographics, Brief Symptom Rating Scale, Interpersonal Interaction Scale (IIS), Frenchay Activities Index, and UCLA Loneliness Scale (UCLA).ResultsA total of 178 participants were recruited in this study. Results indicated that gender, whether participants eat alone or with others at dinner, social media use, perceived economic status, and IIS score were significantly correlated with the loneliness score on the UCLA. Furthermore, there was a significant increase in the loneliness score among male participants in the low loneliness group from baseline to 6 months follow‐up.ConclusionsGender, presence of others at dinner, social media use, perceived economic status, and interpersonal interaction skills are significant factors that influence loneliness among older people using homecare services. Men tend to experience higher levels of loneliness over time.
Integrability and solvability of polynomial Liénard differential systems
Authors: Demina Maria V. 
Published: December 2022

AbstractWe provide the necessary and sufficient conditions of Liouvillian integrability for Liénard differential systems describing nonlinear oscillators with a polynomial damping and a polynomial restoring force. We prove that Liénard differential systems are not Darboux integrable excluding subfamilies with certain restrictions on the degrees of the polynomials arising in the systems. We demonstrate that if the degree of a polynomial responsible for the restoring force is greater than the degree of a polynomial producing the damping, then a generic Liénard differential system is not Liouvillian integrable with the exception of linear Liénard systems. However, for any fixed degrees of the polynomials describing the damping and the restoring force we present subfamilies possessing Liouvillian first integrals. As a by‐product of our results, we find a number of novel Liouvillian integrable subfamilies. In addition, we study the existence of nonautonomous Darboux first integrals and nonautonomous Jacobi last multipliers with a time‐dependent exponential factor.
Combination therapy with human amniotic epithelial cells and hyaluronic acid promotes immune balance recovery in type 1 diabetic rats through local engraftment
Authors: Cheng Ya‐Wei , Luo Yi , Zheng Shu‐Juan , Xiao Jian‐Hui 
Published: December 2022

AbstractStem cell engraftment is currently a promising approach for type 1 diabetes mellitus (T1DM) treatment. In our previous study, engraftment of a combination of human amniotic epithelial cells (hAECs) and hyaluronic acid (HA) showed potent anti‐diabetic effect in streptozotocin (STZ)‐induced T1DM mice via tail vein injection. Here, we adopted a different route of stem cell delivery, that is via pancreatic subcapsular transplantation. This combined local engraftment of hAECs and HA in STZ‐induced T1DM rats showed potent anti‐diabetic activity, leading to stronger hypoglycaemia, more intact islet structure and increased number of insulin‐positive cells compared with those with hAECs or insulin treatments. Engraftment of hAECs alone increased the proportion of Th1 and T‐reg cells and decreased the proportion of Th2 and Th17 cells to protect islet β cells in STZ‐induced T1DM rats, whereas the combined engraftment of hAECs and HA showed more potent regulatory capacity, considerably decreased the level of TNF‐α and IL‐17 and increased the level of TGF‐β1 compared with those by other treatments. The potent synergistic effect of HA contributed to the recovery of immune balance in the diabetic rat model, thereby suggesting a new strategy for effective treatment of T1DM.
Immune checkpoint analysis of T‐cell responses to pp65 and IE‐1 antigens in end‐stage lung diseases
Authors: Bergantini Laura , d'Alessandro Miriana , Cavallaro Dalila , Pordon Elena , Cassai Lucia , Gangi Sara , Meloni Federica , Montagnani Francesca , Paladini Piero , Refini Rosa Metella , Luzzi Luca , Fossi Antonella , Bargagli Elena , Bennett David 
Published: December 2022

AbstractLung transplant (LTX) patients are at high risk of cytomegalovirus (CMV) infection, which is often associated with high mortality and morbidity. Reactivation of CMV causes cell injury due to the cytopathic effect of viral replication and triggering of T cell immunity. The aim of this study was to compare expression of immune checkpoints (ICs) (PD‐1, CTLA‐4, LAG‐3 and TIGIT) in CD4, CD8 and CD56 and activation markers CD137, CD154 and CD69 of end‐stage patients awaiting lung transplant. Eighteen pre‐LTX positive for anti‐CMV IgG titres and 18 healthy subjects were enrolled. IC and activation markers have been evaluated through flow cytometric analysis in HC and pre‐LTX patients. Reactive (QF+) and unreactive (QF−) patients were stratified according to QuantiFERON‐CMV assays. ICs' and activation markers' expression were determined before and after in vitro stimulation with pp‐65 and IE‐1 antigens. Lower expression of PD‐1 was observed in CD4 and CD8 cells of pre‐LTX patients than controls, whereas CTLA4 appeared upregulated in CD56 and CD8 cells. TIGIT is increased on the surface of CD4, CD8 and NK cells after peptide stimulation in QF‐negative patients and PD‐1 is only downregulated after stimulation in the QF‐positive patients. This study provides new evidence of immune dysregulation in patients with end‐stage lung disorders, particularly in relation to immune checkpoint cell biology. The change in QF+ mostly happens on cytotoxic cells NK and CD8, while the changes in QF− were observed in adaptive immune cells, including CD4 and CD8.
Identification of tumour antigens and immune subtypes in the development of an anti‐cancer vaccine for endometrial carcinoma
Authors: Feng Jianyang , He Hong 
Published: December 2022

AbstractTherapeutic application of vaccines to endometrial carcinoma (EC) remains uncertain. In this study, we aimed to identify potential tumour antigens for use in the development of an anti‐tumour mRNA vaccine and clarify immune subtypes and their characteristics for immunotherapeutic application in heterogeneous EC by integrating multi‐omics data. Importantly, four potential tumour antigen candidates—PGR, RBPJ, PARVG and MSX1—were identified and significantly correlated with better overall survival, disease‐free survival and distinct antigen‐presenting cell infiltration in EC. In addition, two different immune subtypes by consensus clustering analysis of the immune‐related genes were identified. Patients with C2 immunophenotypes exhibited superior survival outcomes and ‘hot’ immunoreactivity and harboured higher microsatellite instability scores and tumoral mutation burden but lower copy‐number variation burden. Furthermore, the distinct expression of immunogenic cell death modulators and differential microenvironmental characteristics of immune‐cell infiltration were also revealed between C1 and C2 immune‐subtype tumours. Enrichment analysis of the co‐expression of immune‐related genes showed enrichment in immune response, immune cell‐mediated immunity and antigen processing pathways. These results indicated that these identified tumour antigens can be used for developing antitumour mRNA vaccines, and tumours with C2 immunophenotypic characteristics demonstrated sensitivity and susceptibility to immunotherapy in EC.
Efficacy of a home‐used high‐intensity focused ultrasound device on wrinkle reduction
Authors: Kwack Mi Hee , Lee Weon Ju 
Published: December 2022

AbstractBackgroundHigh‐intensity focused ultrasound (HIFU) has been developed for the treatment of skin wrinkles on the face, neck, and body.ObjectivesThis study aimed to evaluate the effects of a home‐used HIFU device on wrinkles in mice based on the expression of fibrosis‐related genes and proteins.MethodsThe backs of 20‐week‐old mice were treated with a home‐used HIFU using the following probes: 4 MHz, 1.5 mm focal depth. The treated mice were compared with young mice by histological examination, real‐time polymerase chain reaction (PCR), and immunohistochemistry. Histological examination was performed by trichrome staining. Real‐time PCR and immunohistochemistry were conducted to determine the expression of collagen types I and III, matrix metalloproteinase (MMP)‐1, and tissue inhibitor of metalloproteinase (TIMP)‐1.ResultsDermal thickness was increased after treatment with the home‐used HIFU device at 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks on trichrome. Gene and protein expression of collagen types I and III and elastin were increased after treatment with HIFU at all options of 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks. Gene and protein expressions of MMP‐1 and TIMP‐1 were decreased after treatment with HIFU device at 30 and 60 s per day for 1 week or 30 and 60 s per day for 2 weeks.ConclusionThe home‐used HIFU device can be an effective therapeutic modality for skin tightening.
From Athens to Cardiff to Corwen: Ministerial formation in the real world
Authors: James Manon Ceridwen 
Published: December 2022

AbstractThis article explores the delivery and pedagogy of initial ministerial training for lay and ordained candidates in the Church in Wales by its training arm, the St. Padarn's Institute. I discuss how logistical challenges force us into a more pragmatic approach where practical considerations are as influential as philosophical and pedagogical commitments. The close relationship between the national church and ourselves and a “reflective practice” and “practical wisdom” approach enables us to be responsive and flexible to training needs. Although wary of geographical locations being used as shorthand theoretical terms for pedagogies (e.g., Athens, Berlin), I describe our approach as Athens character formation but ultimately locate our pedagogy (following Cronshaw) within the lived experience and landscape of Wales.
Framework for refugee and migrant health research in the WHO European Region
Authors: MacFarlane Anne , Puthoopparambil Soorej Jose , Waagensen Elisabeth , Sisti Leuconoe Grazia , Costanzo Gianfranco , Kayi Ilker , Osseiran Souad , Sakarya Sibel , Severoni Santino , Hannigan Ailish 
Published: December 2022

AbstractMigration is a longstanding, growing global phenomenon. As a social determinant of health, migration can lead to health inequities between people on the move and host populations. Thus, it is imperative that there is a coordinated effort to advance migration‐ and health‐related goals. WHO has a specific remit to support evidence‐based decision‐making in its Member States. As part of that remit, WHO Europe presents this Framework for Refugee and Migrant Health Research in the WHO European Region. It is designed as a starting point for debating and analysing a broad range of options and approaches to help inform a WHO global research agenda on health and migration. This is important because refugee and migrant health research is a complex interdisciplinary field that is expanding in a fast‐changing socio‐political environment. The Framework is intended for all stakeholders involved: academic, civil society organisations, refugees, migrants, policy‐makers, healthcare providers, educators and funders. It is developed by academics in consultation with these stakeholder groups. It reflects on three specific interrelated dynamics in research practice. These are (i) research prioritisation; (ii) study samples and (iii) research design. The Framework offers recommendations to consider for each one of these. It elucidates the value of involving refugees and migrants in research and research agendas and the need to develop an ecosystem that will support and sustain participatory, interdisciplinary, transdisciplinary and inter‐sectoral projects.
Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis
Authors: Zhou Yanli , Fan Wei , Zhang Haoyue , Zhang Jingling , Zhang Guanghui , Wang Ding , Xiang Guisheng , Zhao Changhong , Li Lianhua , He Simei , Lu Yingchun , Zhao Jiuxia , Meng Zhengui , Zhang Xianmin , Meng Hengling , Yin Xinhua , Yang Shengchao , Long Guangqiang 
Published: December 2022

SUMMARYMarsdenia tenacissima is a medicinal plant widely distributed in the calcium‐rich karst regions of southwest China. However, the lack of a reference genome has hampered the implementation of molecular techniques in its breeding, pharmacology and domestication. We generated the chromosome‐level genome assembly in Apocynaceae using combined SMRT sequencing and Hi‐C. The genome length was 381.76 Mb, with 98.9% of it found on 11 chromosomes. The genome contained 222.63 Mb of repetitive sequences and 21 899 predicted gene models, with a contig N50 of 6.57 Mb. Phylogenetic analysis revealed that M. tenacissima diverged from Calotropis gigantea at least 13.43 million years ago. Comparative genomics showed that M. tenacissima underwent ancient shared whole‐genome duplication. This event, together with tandem duplication, contributed to 70.71% of gene‐family expansion. Both pseudogene analysis and selective pressure calculations suggested calcium‐related adaptive evolution in the M. tenacissima genome. Calcium‐induced differentially expressed genes (DEGs) were mainly enriched in cell‐wall‐related processes. Domains (e.g. Fasciclin and Amb_all) and cis‐elements (e.g. MYB and MYC) frequently occurred in the coding and promoter regions of cell‐wall DEGs, respectively, and the expression levels of these genes correlated significantly with those of calcium‐signal‐related transcription factors. Moreover, calcium addition increased tenacissoside I, G and H contents. The availability of this high‐quality genome provides valuable genomic information for genetic breeding and molecular design, and lends insights into the calcium adaptation of M. tenacissima in karst areas.
Biased mutations and gene losses underlying diploidization of the tetraploid broomcorn millet genome
Authors: Sun Yanling , Liu Yang , Shi Jinfeng , Wang Lun , Liang Chengzhi , Yang Jun , Chen Jinfeng , Chen Mingsheng 
Published: December 2022

SUMMARYBroomcorn millet (Panicum miliaceum L.) is one of the earliest domesticated crops, and is a valuable resource to secure food diversity and combat drought stresses under the global warming scenario. However, due to the absence of extant diploid progenitors, the polyploidy genome of broomcorn millet remains poorly understood. Here, we report the chromosome‐scale genome assembly of broomcorn millet. We divided the broomcorn millet genome into two subgenomes using the genome sequence of Panicum hallii, a diploid relative of broomcorn millet. Our analyses revealed that the two subgenomes diverged at ~4.8 million years ago (Mya), while the allotetraploidization of broomcorn millet may have occurred about ~0.48 Mya, suggesting that broomcorn millet is a relatively recent allotetraploid. Comparative analyses showed that subgenome B was larger than subgenome A in size, which was caused by the biased accumulation of long terminal repeat retrotransposons in the progenitor of subgenome B before polyploidization. Notably, the accumulation of biased mutations in the transposable element‐rich subgenome B led to more gene losses. Although no significant dominance of either subgenome was observed in the expression profiles of broomcorn millet, we found the minimally expressed genes in P. hallii tended to be lost during diploidization of broomcorn millet. These results suggest that broomcorn millet is at the early stage of diploidization and that mutations likely occurred more on genes that were marked with lower expression levels.