bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence in situ hybridization test for detection of endometrial carcinoma cells by non‐invasive vaginal swab
Authors: Weimer Jörg , Hüttmann Martje , Nusilati Asiyan , Andreas Svenja , Röseler Jona , Tribian Nils , Rogmans Christoph , Stope Matthias Bernhard , Dahl Edgar , Mustea Alexander , Stickeler Elmar , Hedemann Nina , Flörkemeier Inken , Tiemann Katharina , Magadeeva Svetlana , Dempfle Astrid , Arnold Norbert , Maass Nicolai , Bauerschlag Dirk 
Published: January 2023

AbstractEndometrial cancer (EC) is the most common gynaecological malignancy with increasing incidence in developed countries. As gold standard, hysteroscopy confirms only 30% of suspected ECs. The detection of EC cells in the vagina by fluorescence in situ hybridization (FISH) after a smear test could reduce invasive procedures in the future. Using array‐based comparative genome hybridization (aCGH) on 65 endometrial carcinomas, most frequently imbalanced regions of the tumour genome were identified. Bacterial artificial chromosomes were used to generate FISH‐probes homologue to these human regions. The FISH test was hybridized on swabs specimens collected from the vaginal cavity. Samples from six patients without EC were selected as a negative control and on 13 patients with known EC as a positive control. To distinguish between benign and EC cases, the cut‐off value has been defined. A first validation of this EC‐FISH Test was performed with swabs from 41 patients with suspected EC. The most common genomic imbalances in EC are around the CTNNB1, FBXW7 and APC genes. The cut‐off is defined at 32% of analysed cells without diploid signal pattern. This differs significantly between the positive and negative controls (p < 0.001). In a first validation cohort of 41 patients with suspected EC, the EC‐FISH Test distinguishes patients with and without EC with a sensitivity of 91% and a specificity of 83%. The negative predictive value is 96%. This is the first report of a non‐invasive EC‐FISH Test to predict EC in women with suspected EC.
The effects of KTKEGV repeat motif and intervening ATVA sequence on α‐synuclein solubility and assembly
Authors: Brontesi Lisa , Imberdis Thibaut , Ramalingam Nagendran , Dettmer Ulf 
Published: January 2023

AbstractAlpha‐synuclein (αS), the key protein in Parkinson's disease, is typically described as an intrinsically disordered protein. Consistent with this notion, several context‐dependent folding states may coexist in neurons. Unfolded soluble monomers, helical monomers at membranes and helical multimers (soluble or at membranes) have all been reported and may be in an equilibrium with each other. We previously found that αS can be stabilized in its membrane‐associated monomeric form by genetically increasing the hydrophobicity of the membrane‐embedded half of the αS helix. αS amphipathic helix formation at membranes is governed by up to nine 11‐amino acid repeats with the core motif KTKEGV. However, this repeat is only imperfectly conserved; for example, it consists of KAKEGV in repeat #1, KTKEQV in repeat #5, and AVVTGV in the poorly conserved repeat #6. Here we explored the effect of perfecting the αS core repeat to nine times KTKEGV (“9KV”) and found by sequential protein extraction that this engineered mutant accumulates in the cytosolic phase of neural cells. Intact‐cell cross‐linking trapped a part of the cytosolic portion at multimeric positions (30, 60, 80, 100 kDa). Thus, compared to wild‐type αS, αS 9KV seems less prone to populating the membrane‐associated monomeric form. Removing the “ATVA” intervening amino‐acid sequence between repeats 4 and 5 slightly increased cytosolic localization while adding “ATVA” in between all repeats 1–8 caused αS to be trapped as a monomer in membrane fractions. Our results contribute to an ongoing debate on the dynamic structure of αS, highlighting that wild‐type αS is unlikely to be fully multimeric/monomeric or fully cytosolic/membrane‐associated in cells, but protein engineering can create αS variants that preferentially adopt a certain state. Overall, the imperfect nature of the KTKEGV repeat motifs and the presence of ATVA in between repeats 4 and 5 seem to prevent a strong cytosolic localization of αS and thus play a major role in the protein's ability to dynamically populate cytosolic vs. membrane‐associated and monomeric vs. multimeric states.image
The amino acid and polycyclic aromatic hydrocarbon compositions of the promptly recovered CM2 Winchcombe carbonaceous chondrite
Authors: Chan Queenie H. S. , Watson Jonathan S. , Sephton Mark A. , O'Brien Áine C. , Hallis Lydia J. 
Published: January 2023

AbstractThe rapid recovery of the Winchcombe meteorite offers a valuable opportunity to study the soluble organic matter (SOM) profile in pristine carbonaceous astromaterials. Our interests in the biologically relevant molecules, amino acids—monomers of protein, and the most prevalent meteoritic organics—polycyclic aromatic hydrocarbons (PAHs) are addressed by analyzing the solvent extracts of a Winchcombe meteorite stone using gas chromatography mass spectrometry. The Winchcombe sample contains an amino acid abundance of ~1132 parts‐per‐billion that is about 10 times lower than other CM2 meteorites. The detection of terrestrially rare amino acids, including α‐aminoisobutyric acid (AIB); isovaline; β‐alanine; α‐, β‐, and γ‐amino‐n‐butyric acids; and 5‐aminopentanoic acid, and the racemic enantiomeric ratios (D/L = 1) observed for alanine and isovaline indicate that these amino acids are indigenous to the meteorite and not terrestrial contaminants. The presence of predominantly α‐AIB and isovaline is consistent with their formation via the Strecker‐cyanohydrin synthetic pathway. The L‐enantiomeric excesses in isovaline previously observed for aqueously altered meteorites were viewed as an indicator of parent body aqueous processing; thus, the racemic ratio of isovaline observed for Winchcombe, alongside the overall high free:total amino acid ratio, and the low amino acid concentration suggest that the analyzed stone is derived from a lithology that has experienced brief episode(s) of aqueous alteration. Winchcombe also contains 2‐ to 6‐ring alkylated and nonalkylated PAHs. The low total PAHs abundance (6177 ppb) and high nonalkylated:alkylated ratio are distinct from that observed for heavily aqueously altered CMs. The weak petrographic properties of Winchcombe, as well as the discrepancies observed for the Winchcombe SOM content—a low total amino acid abundance comparable to heavily altered CMs, and yet the high free:total amino acid and nonalkylated:alkylated PAH ratios are on par with the less altered CMs—suggest that Winchcombe could represent a class of weak, poorly lithified meteorite not been previously studied.
Xanthomonas campestris VemR enhances the transcription of the T3SS key regulator HrpX via physical interaction with HrpG
Authors: Li Rui‐Fang , Peng Jian‐Ling , Liu Qian‐Qian , Chang Zheng , Huang Yi‐Xin , Tang Ji‐Liang , Lu Guang‐Tao 
Published: January 2023

AbstractVemR is a response regulator of the two‐component signalling systems (TCSs). It consists solely of a receiver domain. Previous studies have shown that VemR plays an important role in influencing the production of exopolysaccharides and exoenzymes, cell motility, and virulence of Xanthomonas campestris pv. campestris (Xcc). However, whether VemR is involved in the essential pathogenicity determinant type III secretion system (T3SS) is unclear. In this work, we found by transcriptome analysis that VemR modulates about 10% of Xcc genes, which are involved in various cellular processes including the T3SS. Further experiments revealed that VemR physically interacts with numerous proteins, including the TCS sensor kinases HpaS and RavA, and the TCS response regulator HrpG, which directly activates the transcription of HrpX, a key regulator controlling T3SS expression. It has been demonstrated previously that HpaS composes a TCS with HrpG or VemR to control the expression of T3SS or swimming motility, while RavA and VemR form a TCS to control the expression of flagellar genes. Mutation analysis and in vitro transcription assay revealed that phosphorylation might be essential for the function of VemR and phosphorylated VemR could significantly enhance the activation of hrpX transcription by HrpG. We infer that the binding of VemR to HrpG can modulate the activity of HrpG to the hrpX promoter, thereby enhancing hrpX transcription. Although further studies are required to validate this inference and explore the detailed functional mechanism of VemR, our findings provide some insights into the complex regulatory cascade of the HpaS/RavA‐VemR/HrpG‐HrpX signal transduction system in the control of T3SS.
OsGLP3‐7 positively regulates rice immune response by activating hydrogen peroxide, jasmonic acid, and phytoalexin metabolic pathways
Authors: Sun Bingrui , Li Wenyan , Ma Yamei , Yu Ting , Huang Wenjie , Ding Jierong , Yu Hang , Jiang Liqun , Zhang Jing , Lv Shuwei , Yang Jianyuan , Yan Shijuan , Liu Bin , Liu Qing 
Published: January 2023

AbstractAlthough germin‐like proteins (GLPs) have been demonstrated to participate in plant biotic stress responses, their specific functions in rice disease resistance are still largely unknown. Here, we report the identification and characterization of OsGLP3‐7, a member of the GLP family in rice. Expression of OsGLP3‐7 was significantly induced by pathogen infection, jasmonic acid (JA) treatment, and hydrogen peroxide (H2O2) treatment. OsGLP3‐7 was highly expressed in leaves and sublocalized in the cytoplasm. Overexpression of OsGLP3‐7 increased plant resistance to leaf blast, panicle blast, and bacterial blight, whereas disease resistance in OsGLP3‐7 RNAi silenced plants was remarkably compromised, suggesting this gene is a positive regulator of disease resistance in rice. Further analysis showed that OsGLP3‐7 has superoxide dismutase (SOD) activity and can influence the accumulation of H2O2 in transgenic plants. Many genes involved in JA and phytoalexin biosynthesis were strongly induced, accompanied with elevated levels of JA and phytoalexins in OsGLP3‐7‐overexpressing plants, while expression of these genes was significantly suppressed and the levels of JA and phytoalexins were reduced in OsGLP3‐7 RNAi plants compared with control plants, both before and after pathogen inoculation. Moreover, we showed that OsGLP3‐7‐dependent phytoalexin accumulation may, at least partially, be attributed to the elevated JA levels observed after pathogen infection. Taken together, our results indicate that OsGLP3‐7 positively regulates rice disease resistance by activating JA and phytoalexin metabolic pathways, thus providing novel insights into the disease resistance mechanisms conferred by GLPs in rice.
Theoretical performance of progressive addition lenses with poorly measured individual parameters
Authors: Pascual Eduardo , Gómez‐Pedrero José A. , Alonso José 
Published: January 2023

AbstractPurposeThe aim of this paper was to present a theoretical study of how poorly measured individual parameters affect the optical performance of progressive addition lenses (PALs). Modern progressive lenses can be prescribed based on parameters such as vertex distance, pantoscopic and wrap angles. These parameters can be measured from the lens wearer using specific devices; however, not all of them can be measured with the same precision, and the impact of measurement errors on the lens performance is still unknown.MethodsData from 1900 patients were used to simulate the performance of four PAL designs with different degrees of complexity: perfect individual design, individual design with induced errors in the individual parameters, optimised design and conventional/basic design. For each patient and design, a quality metric was calculated to describe the optical performance of the lens.ResultsThe design having the best performance was the perfect individual design, followed by the individual design with induced errors, the optimised design and finally the conventional/basic design.ConclusionsIndividual designs with measurement errors have better optical performance than lenses with less complexity, such as the optimised or conventional designs. This knowledge is useful for the eye care professional to make informed choices when dispensing these lenses.
Correlation of horizontal condylar angle measured with CBCT and clinical dysfunction index in patients with temporomandibular disorders
Authors: Khojastepour Leila , Paknahad Maryam , Rastegarian Navid , Mahjoori‐Ghasrodashti Mohammad , Vojdani Mahroo , Omidi Mahsa 
Published: January 2023

AbstractObjectivesThis study aimed to find a correlation between the severity of TMD based on the Helkimo clinical dysfunction index and the mean horizontal condylar angle (HCA) in CBCT images.Materials and methodsHCAs of 84 patients with TMD were measured in CBCT cross‐sections. The TMD patients were clinically evaluated and then subdivided into three groups using Helkimo clinical Di and. Means of HCA and differences between right and left HCA were compared with a matched control group and among TMD patients with different TMD severity.ResultsThe difference between right and left HCA and HCA mean were significantly greater in TMD patients than in the control group. The mean of HCA was also significantly different among TMD patients with different Di.ConclusionIt was revealed significant differences between TMD and non‐TMD condyles. There was also a significant relationship between the Helkimo clinical Di and the greater HCA.
Parent genome contribution and its relationship with grain yield in backcross‐derived lines of soybean (Glycine max)
Authors: Koraboyana Vijayakumar , Gill Balwinder Singh , Sirari Asmita , Khosla Gaurav , Bindra Shayla , Kumar Vineet 
Published: January 2023

AbstractIntrogression of yellow mosaic disease (YMD) resistance and effect of recurrent parent genome (RPG) on grain yield was studied in 84 soybean genotypes from four populations namely, F2:7, BC1F6, BC2F5 and BC3F4 derived from cross JS335 x SL525. It was observed that in F2:7, BC1F6, BC2F5 and BC3F4 derived lines, RPG contribution was 42.5%, 54.9%, 66.4% and 77.6%, respectively, which is significantly less than expected values. Linkage drag from donor parent with YMD resistance gene may be a possible reason for such deviations. Average grain yield per plant in F2:7, BC1F6, BC2F5 and BC3F4 generations was observed as 13.0, 14.3, 14.9 and 16.1 g, respectively. It was observed that genotypes with more than 80% RPG observed to have both YMD resistance and good yield potential. Graphical genotyping (GGT) analysis revealed that maximum RPG was recovered in chromosomes 8 and 10 and maximum introgression occurred in chromosomes 6 and 19. Our results demonstrated that RPG was positively associated with yield as evident from yield increase with increase in RPG.
Well‐posedness of generalized KdV and one‐dimensional fourth‐order derivative nonlinear Schrödinger equations for data with an infinite L2 norm
Authors: Lu Yufeng 
Published: January 2023

AbstractWe study the Cauchy problem for the generalized Korteweg–de Vries (KdV) and one‐dimensional fourth‐order derivative nonlinear Schrödinger equations, for which the global well‐posedness of solutions with small rough data in certain scaling limit of modulation spaces is shown, which contain some data with infinite L2 norm.
Living wages in Portugal: In search of dignity in a polarised labour market
Authors: Pereirinha José António , Pereira Elvira 
Published: January 2023

AbstractLiving wage (LW) is a concept that goes beyond that of national minimum wage (NMW), since it implies income adequacy to the wage earners and to his/her family members. It is coherent with the principle related to wages of the European Pillar of Social Rights (EPSR) namely the right of workers to fair wages, and the duty to ensure adequate minimum wages providing the satisfaction of the worker needs and of his/her family, which originated a proposal of the European Commission for a Directive on adequate minimum wages in the European Union. This article discusses the possibility of implementation of a LW policy in Portugal, a country with low average and median wages, a generous NMW relative to average and median wage, high earnings inequality and a polarised labour market. To be defensible, this policy should reach household income adequacy, be feasible regarding the labour and fiscal costs, and be socially acceptable regarding the change of earnings distribution. The discussion of this policy is made using EU‐SILC data and data from interviews with social partners involved in the national level social dialogue. We quantify and qualify some of the trade‐offs, simulating different values for core policy variables, centred on the worker as a wage earner, as a household member and as a citizen with social rights and fiscal duties, supported on an adequate normative estimation of a consensual Minimum Income Standard (MIS) for the Portuguese households.
The Impact of Municipality Mergers on Political Participation
Authors: Frey Bruno S. , Briviba Andre , Gullo Anthony 
Published: January 2023

AbstractThe citizen assembly of the Swiss canton Glarus in 2006 decided to radically reduce the historical number of communes from 25 to three. Studies show that the financial and economic consequences are surprisingly small. This paper analyzes the effects on citizens' vote participation as an indicator of their political involvement. Using the difference‐in‐difference and the synthetic control methods, we empirically show that vote participation dropped significantly, also compared to neighboring cantons and the rest of Switzerland.
Functional validation of co‐culture model of human keratinocytes and neuronal cell line for sensitive skin by using transient receptor potential channel vanilloid subfamily member 1 antagonist
Authors: Shin Sun Mee , Baek Eun Joo , Oh Dong Yeol , Kim Kwang Ho , Kim Kwang Joong , Park Eun Joo 
Published: January 2023

AbstractBackgroundSensitive skin is a subjective cutaneous hyper‐reactivity that occurs in response to various innocuous stimuli. Keratinocytes have recently been shown to participate in sensory transduction by releasing many neuroactive molecules that bind to intra‐epidermal free nerve endings and modulate nociception. In the literature, the characterization of these interactions has been based on the co‐culture of keratinocyte and mammalian‐origin neuronal cell lines. In this study, we established an in vitro model based on a co‐culture of primary human keratinocytes and differentiated SH‐SY5Y cells, a human neuronal cell line.MethodsHuman epidermal keratinocytes and SH‐SY5Y cells were monocultured and co‐cultured. Changes in calcium influx, substance P, inflammatory cytokines, and neuropeptides between the monoculture and co‐culture groups treated with capsaicin only and capsaicin with transient receptor potential channel vanilloid subfamily member 1 (TRPV1) antagonist, trans‐4‐tert‐butylcyclohexanol (TTBC), together. In addition, the difference in stinging sensation was evaluated by applying it to the volunteers.ResultsWhen SH‐SY5Y cells were co‐cultured with keratinocytes, they had no significant effect on axonal development. Substance P was also released after capsaicin treatment and reduced by TTBC under co‐culture conditions. Moreover, the expression of inflammatory cytokines and neuropeptides was significantly increased in co‐cultured keratinocytes compared to that under monoculture conditions. In addition, the stinging sensation was significantly induced after the application of capsaicin in vivo and was relieved after the application of the TRPV1 antagonist.ConclusionWe demonstrated that the novel co‐culture model is functionally valid through capsaicin and TRPV1 antagonist. We also confirmed that TTBC could be used for the treatment of sensitive skin through a co‐culture model and in vivo tests. This co‐culture model of keratinocytes and SH‐SY5Y cells may be useful in vitro alternatives for studying the close communication between keratinocytes and neuronal cells and for screening therapeutic drugs for sensitive skin.
Patient perspectives on intraoperative blood transfusion: A qualitative interview study with perioperative patients
Authors: Lenet Tori , Skanes Stephanie , Tropiano Joseph , Verret Michael , McIsaac Daniel I. , Tinmouth Alan , Hallet Julie , Nicholls Stuart G. , Fergusson Dean A. , Martel Guillaume 
Published: January 2023

AbstractBackgroundWhile red blood cell (RBC) transfusions are frequently administered during surgery, little is known about patient perspectives regarding intraoperative transfusion. The aim of this study was to understand patient perspectives about intraoperative RBC transfusion and explore their willingness to engage in transfusion prevention strategies.Study Design and MethodsThis descriptive qualitative study used semi‐structured patient interviews before and after surgery. Purposive sampling was used to select adult patients with varying perioperative courses, including having perioperative transfusion or postoperative anemia. Inductive and deductive thematic analyses were conducted to identify themes.ResultsTwenty patients (nine preoperative and 11 postoperative patients) were interviewed. The following themes were identified: Risk–benefit perception of transfusion, transfusion acceptance, trust, patient involvement in transfusion decisions, acceptance of transfusion prevention interventions, and communication. Patients perceived transfusions as low‐risk compared to the surgery itself. Factors influencing transfusion acceptance included trust in the healthcare system and the perception of the treatability of transfusion‐related complications. Some patients preferred to defer transfusion decision making to the perioperative team, citing trust in professional judgment and building a positive relationship with their surgeon. Others wished for their preferences to be incorporated into transfusion decisions. Some desired detailed blood consent conversations and most were willing to participate in strategies to reduce intraoperative transfusion.ConclusionIn our sample, patients consider intraoperative transfusions as low‐risk high‐reward interventions and trust the healthcare system and perioperative team to guide intraoperative transfusion decision making. However, preoperative transfusion consent discussions were recalled as being superficial and lacking nuance. Targeted strategies are required to improve blood consent discussions to better integrate patient preferences.
Ramsey numbers of Boolean lattices
Authors: Grósz Dániel , Methuku Abhishek , Tompkins Casey 
Published: January 2023

AbstractThe poset Ramsey number is the smallest integer such that any blue–red coloring of the elements of the Boolean lattice has a blue‐induced copy of or a red‐induced copy of . The weak poset Ramsey number is defined analogously, with weak copies instead of induced copies. It is easy to see that . Axenovich and Walzer (Order 34 (2017), 287–298) showed that . Recently, Lu and Thompson (Order 39 (2022), no. 2, 171–185) improved the upper bound to . In this paper, we solve this problem asymptotically by showing that . In the diagonal case, Cox and Stolee (Order 35 (2018), no. 3, 557–579) proved using a probabilistic construction. In the induced case, Bohman and Peng (arXiv preprint arXiv:2102.00317, 2021) showed using an explicit construction. Improving these results, we show that for all and large by giving an explicit construction; in particular, we prove that .