bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiovascular and psychosocial risks among young patients with Acute Myocardial Infarction
Authors: Faresjo Ashild , Karlsson Jan-Erik , Segerberg Henrik , Faresjo Tomas , Lebena Andrea 
Published: January 2023

Abstract
Background: Despite improvements in the treatment and prevention of cardiovascular disease since the 1960s, the incidence of cardiovascular diseases among young people has remained the same for many years. This study aimed to compare the clinical and psychosocial attributes of young persons affected by myocardial infarction under the age of 50 years compared to middle-aged myocardial infarction patients 51-65 years old.
Methods: Data from patients with a documented STEMI or NSTEMI elevated acute myocardial infarction in the age groups up to 65 years, were collected from cardiology clinics at three hospitals in southeast Sweden. The Stressheart study comprised a total of 213 acute myocardial infarction patients, of which n=33 (15.5%) were under 50 years of age and n=180 (84.5%) were middle-aged, (51-65 years). These acute myocardial infarction patients filled in a questionnaire at discharge from the hospital and further information through documentation of data in their medical records.
Results: Blood pressure was significantly higher in young compared to middle-aged patients. For diastolic blood pressure (p=0.003), systolic blood pressure (p=0.028), and mean arterial pressure (p=0.005). Young AMI patients had a higher (p=0.030) body mass index (BMI) than the middle-aged. Young AMI patients were reported to be more stressed (p=0.042), had more frequently experienced a serious life event the previous year (p=0.029), and felt less energetic (p=0.044) than middle-aged AMI patients.
Conclusions: This study revealed, that persons under the age of 50 affected by acute myocardial infarction exhibit traditional cardiovascular risk factors like high blood pressure, and higher BMI, and were more exposed to some psychosocial risk factors. The risk profile of these young persons affected by AMI was in some respects more exaugurated than for middle-aged persons with AMI. This study underlines the importance of the early discovery of those at increased risk and encourages preventative actions to focus on both clinical and psychosocial risk factors.
Reducing anastomotic leakage in TaTME by mucosal coverage of staple lines: a pilot study with preliminary results.
Authors: Deguchi Katsuya , Hirano Yasumitsu , Okazaki Naoto 
Published: January 2023

Abstract
Purpose
We have performed a single stapled anastomosis with double purse-string sutures as a Trans anal Total Mesorectal Excision (TaTME) reconstruction for low rectal cancer. We report an attempt to control local infection and reduce anastomotic leakage (AL) at this anastomotic site.
Patients and Methods
Fifty-one patients who underwent TaTME for low rectal cancer from April 2021 to October 2022 were included. TaTME was performed by two teams, and reconstruction was performed by anastomosis with a single stapling technique (SST). After the anastomosis was thoroughly cleaned, Z sutures were placed parallel to the staple line to suture the mucosa on the oral and anal side of the staple line and to cover the staple line circumferentially. Data on operative time, Distal Margin (DM), recurrence and postoperative complications including AL were prospectively collected.
Results
The mean age of patients was 67 years. There were 36 males and 15 females. The overall mean operative time was 283.1 minutes, and the mean Distal Margin was 2.2 cm. Postoperative complications were observed in 5.9% of the patients, but no AL was observed, nor any serious complications with Clavien-Dindo ≥ 3 grade. Of the 49 cases excluding Stage 4, postoperative recurrence was observed in 2 cases (4.9%).
Conclusion
In patients with lower rectal cancer who underwent TaTME, additional mucosal coverage of the anastomotic staple line by transanal manipulation after reconstruction may be associated with a reduction in the incidence of postoperative AL. Further studies including late anastomotic complications are needed.
Prevalence, risk and spatial distribution of schistosomiasis among adults to promote Universal Health Coverage: a cross-sectional study in Madagascar
Authors: Gruninger Sarah Katharina , Rasamoelina Tahinamandranto , Rakotoarivelo Rivo Andry , Razafindrakoto Anjarasoa , Rasolojaona Zaraniaina Tahiry , Rakotozafy Rodson Morin , Soloniaina Patrick Richard , Rakotozandrindrainy Njary , Rausche Pia , Doumbia Cheick Oumar , Jaeger Anna , Zerbo Alexandre , von Thien Heidrun , Klein Philipp , Dam Govert van , Tannich Egbert , Schwarz Norbert Georg , Lorenz Eva , May Jürgen , Rakotozandrindrainy Raphael , Fusco Daniela 
Published: January 2023

Abstract
Background: The goal to eliminate the parasitic disease of poverty schistosomiasis as a public health problem is aligned with the 2030 United Nations agenda for Sustainable Development Goals, including Universal Health Coverage (UHC). Current control strategies focus on school-aged children, systematically neglecting adults. We aimed at providing evidence for the need of shifting the paradigm of schistosomiasis control programs from targeted to generalized approaches as key element for both the elimination of schistosomiasis as a public health problem and the promotion of UHC.
Methods: In a cross-sectional study we determined prevalence and risk factors for schistosomiasis by a semi-quantitative PCR assay from specimens collected from 1,482 adult participants at three primary health-care centers in north-western and central areas of Madagascar.
Results: The highest prevalence of S. mansoni, S. haematobium and co-infection of both species was 59.5%, 61.3% and 3.3%, respectively. Higher prevalence was observed among males and main contributors to the family income. Not working as a farmer and higher age were found to be protective factors for infection.
Conclusions: Our findings provide evidence that adults are a high-risk group for schistosomiasis. The general focus on children and the exclusion of adults from control strategies for schistosomiasis in high endemic countries may hamper the achievement of both the schistosomiasis elimination goal and UHC. Our data suggests that, for ensuring basic health as a human right, current public health strategies for schistosomiasis prevention and control need to be re-addressed towards more context specific, holistic and integrated approaches.
Potential effect of Chloroquine and Propranolol combination to treat colorectal and triple-negative breast cancers
Authors: Emanuel Anselmino Luciano , Virginia Baglioni María , Georgina Reynoso , Rosa Rozados Viviana , Graciela Scharovsky Olga , José Rico María , Mauricio Menacho-Márquez 
Published: January 2023

Abstract
Drug repositioning explores the reuse of non-cancer drugs to treat tumors. In this work, we evaluated the effect of the combination of chloroquine and propranolol on colorectal and triple-negative breast cancers.
Using as in vitromodels the colorectal cancer cell lines HCT116, HT29, and CT26, and as triple-negative breast cancer models the 4T1, M-406, and MDA-MB-231 cell lines, we evaluated the effect of the drugs combination on the viability, apoptosis, clonogenicity, and cellular migratory capacity. To explore the in vivo effects of the combination on tumor growth and metastasis development we employed graft models in BALB/c, nude, and CBi mice.
In vitro studies showed that combined treatment decreased cell viability in a dose-dependent manner and increased apoptosis. Also, we demonstrated that these drugs act synergically and that it affects clonogenicity and migration. In vivo studies indicated that this drug combination was effective on colorectal models but only partially on breast cancer. These results contributed to the search for new and safe treatments for colorectal and triple-negative carcinomas.
Simultaneous multi-crop land suitability prediction from remote sensing data using semi-supervised learning
Authors: Bhullar Amanjot , Nadeem Khurram , Ali R. Ayesha 
Published: January 2023

Abstract
This study presents an artificial neural network based model that can simultaneously estimate land suitability for barley, peas, spring wheat, canola, oats, and soy in Canada leading to more accurate predictions than single-crop models. The novelties in the modelling method include using an indicator function which allows for a multivariate model to be trained, and a semi-supervised learning approach which allows for training with unlabelled data. The model performs well on land not used in the training set, as demonstrated by both K-fold cross-validation and a visual comparison of crop inventory to predicted land suitability in northern Alberta. The predicted suitability of crops correspond with a region's growing season length; this is in line with literature. Northern Canada is almost completely unused for agriculture, but this may change in the coming decades due to the climate becoming more favorable for agriculture. The model presented in this work can allow for a precise cost benefit analysis regarding environmental damage and economic benefits of cultivating new lands in Canada.
An oral cancer vaccine using a Bifidobacterium vector enhances the efficacy of combination therapy with anti-PD-1 and anti-CTLA-4 antibodies in a mouse renal cell carcinoma model
Authors: Ueki Hideto , Kitagawa Koichi , Kato Mako , Yanase Shihoko , Okamura Yasuyoshi , Bando Yukari , Hara Takuto , Terakawa Tomoaki , Furukawa Junya , Nakano Yuzo , Fujisawa Masato , Shirakawa Toshiro 
Published: January 2023

Abstract
Recently, immune checkpoint inhibitor (ICI) based combination therapies, including anti-PD-1 antibody, nivolumab with anti-CTLA-4 antibody, and ipilimumab have become the primary treatment option for metastatic or unresectable renal cell carcinoma (RCC). However, despite the combination of two ICIs, 60 to 70% of patients are still resistant to first-line cancer immunotherapy.
In the present study, undertook combination immunotherapy for RCC using an oral cancer vaccine (Bifidobacterium longum displaying WT1 tumor associated antigen (B. longum 420)) with anti-PD-1 and anti-CTLA-4 antibodies in a mouse syngeneic model of RCC to explore possible synergistic effects. We found that B. longum 420 significantly improved the survival of mice bearing RCC tumors treated by anti-PD-1 and anti-CTLA-4 antibodies compared to the mice treated by the antibodies alone. This result suggests that B. longum 420 oral cancer vaccine as an adjunct to ICIs could provide a novel treatment option for RCC patients.
Our microbiome analysis revealed that the proportion of Lactobacilli was significantly increased by B. longum 420. Although the detailed mechanism of action is unknown, it is possible that microbiome alteration by B. longum 420 enhances the efficacy of the ICIs.
A Smartphone-based Zero-Effort Method for Mitigating Epidemic Propagation
Authors: Wang Qu , Fu Meixia , Wang Jianquan , Sun Lei , Huang Rong , Li Xianda , Jiang Zhuqing 
Published: January 2023

Abstract
A large number of epidemics, including COVID-19 and SARS, spread through droplets, quickly swept the world and claimed the precious lives of large numbers of people. Building a low-cost and real-time epidemic early warning system to identify individuals who have been in contact with infected individuals and determine whether they need to be quarantined is an effective means to mitigate the spread of the epidemic. In this paper, we propose a smartphone-based zero-effort epidemic warning method for mitigating epidemic propagation. Firstly, we recognize epidemic-related voice activity relevant to epidemics spread by hierarchical attention mechanism and temporal convolutional network. The hierarchical attention mechanism is used to find local important information through global scanning, then enhances useful information and suppresses useless information. Subsequently, we estimate the social distance between users through sensors built-in smartphone. Furthermore, we combine Wi-Fi network logs and social distance to comprehensively judge whether there is spatiotemporal contact between users and determine the duration of contact. Finally, we estimate infection risk based on epidemic-related vocal activity, social distance, and contact time. We conduct a large number of well-designed experiments in typical scenarios to fully verify the proposed method. The proposed method does not rely on any additional infrastructure and historical training data, which is conducive to integration with epidemic prevention and control systems and large-scale applications.
Responsive manganese-based nanoplatform amplifying cGAS-STING activation for immunotherapy
Authors: He Qingbin , Zheng Runxiao , Ma Junchi , Zhao Luyang , Shi Yafang , Qiu Jianfeng 
Published: January 2023

Abstract
Background: The activation of the cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes (cGAS-STING) signaling pathway has attracted great attention for its ability to up-regulate innate immune response and thus enhance cancer immunotherapy. However, many STING agonists limit the further advancement of immunotherapy due to weak tumor responsiveness or low activation efficiency. The responsive and effective activation of cGAS-STING signaling in tumors is a highly challenging process.
Methods: In this study, a manganese-based nanoplatform (MPCZ NPs) was constructed that could responsively and efficiently generate more manganese ions (Mn2+) and reactive oxygen species (ROS) to activate cGAS-STING signaling pathway. Briefly, manganese dioxide (MnO2) was loaded with zinc protoporphyrin IX (ZPP) molecule and coated by polydopamine (PDA) embedded with NH4HCO3 to obtain MPCZ NPs. Additionally, MPCZ NPs were evaluated in vitro and in vivo for their antitumor effects by methyl thiazolyl tetrazolium (MTT) assay and TUNEL assays, respectively.
Results: In this system, tumor responsiveness was achieved by exogenous (laser irradiation) and endogenous (high levels GSH) stimulation, which triggered the collapse or degradation of PDA and MnO2. Moreover, the release of Mn2+ augmented the cGAS-STING signaling pathway and enhanced the conversion of hydrogen peroxide (H2O2) to hydroxyl radical (·OH) under NIR laser irradiation. Furthermore, the release of ZPP and the elimination of GSH by MPCZ NPs inhibited HO-1 activity and prevented ROS consumption, respectively.
Conclusions: This adopted open source and reduce expenditure strategy to effectively generate more ROS and Mn2+ to responsively activate cGAS-STING signaling pathway, providing a new strategy for improving immunotherapy.
Association Between Hypertension and the Prevalence of Liver Steatosis and Fibrosis
Authors: Fu Huanjie , Yu Hao , Zhao Yisheng , Chen Jinhong , Liu Zhaochao 
Published: January 2023

Abstract
Background
Hypertension (HTN) and non-alcoholic fatty liver disease (NAFLD) usually occur together and have some common pathophysiological symptoms. In this study, we determined the relationship between HTN status and the rates of liver steatosis and fibrosis based on the liver stiffness measurement and controlled attenuation parameter obtained by performing liver transient ultrasound elastography (TUE).
Methods
To perform this cross-sectional study, data were obtained from the National Health and Nutrition Examination Survey for 2017-March 2020 Pre-pandemic cycle. The relationship between HTN and the rates of liver steatosis and fibrosis was analyzed by constructing a multivariate logistic regression model. We also conducted subgroup analyses based on the age, gender, ethnicity, and body mass index (BMI) of the patients.
Results
In total, 4,837 participants were recruited, including 2,375 participants with HTN and 2,462 participants without HTN. After adjusting possible confounders, HTN was positively related to the liver steatosis rate (OR = 1.4, 95% CI: 1.1–1.8). Such HTN-associated incidences were higher among males (OR = 1.6, 95% CI: 1.1–2.3), non-Hispanic African American individuals (OR = 1.9, 95% CI: 1.1–3.5), and participants with BMI ≥ 25 < 30 kg/m2 (OR = 1.7, 95% CI: 1.1–2.5). Additionally, HTN was positively associated with the fibrosis rate (OR = 2.0, 95% CI: 1.3–3.0), especially among females (OR = 2.6, 95% CI: 1.3–5.1), among individuals who were 40–59 years old (OR = 2.3, 95% CI: 1.1–4.6), 60–80 years old (OR = 2.2, 95% CI: 1.2–4.1), non-Hispanic Caucasian (OR = 3.0, 95% CI: 1.6–5.9), among those with BMI ≥ 25 < 30 kg/m2 (OR = 3.0, 95% CI: 1.1–8.1), and those with BMI ≥ 30 kg/m2 (OR = 2.0, 95% CI: 1.2–3.3).
Conclusions
The results of this study showed that HTN status was positively associated with liver steatosis and fibrosis rates, especially for subjects with BMI ≥ 25 kg/m2. The relationship was also affected by the ethnicity of the participants.
Predicting ambient PM2.5 concentrations via time series models in Anhui Province, China
Authors: Hasnain Ahmad , Hashmi Muhammad Zaffar , Ahmed Zulkifl , Bhatti Uzair Aslam , Akhter Zaeem Hassan , Min Xiangqiang , Yue Yin , He Yufeng , Nadeem Basit , Wei Geng 
Published: January 2023

Abstract

Due to rapid expansion in the global economy and industrialization, PM2.5 (particles smaller than 2.5 µm in aerodynamic diameter) pollution has become a key environmental issue. The public health and social development directly affected by high PM2.5 levels. In this paper, ambient PM2.5 concentrations along with meteorological data are forecasted using time series models, including random forest (RF), prophet forecasting model (PFM) and autoregressive integrated moving average (ARIMA) in Anhui province, China. The results indicate that the RF model outperformed the PFM and ARIMA in the prediction of PM2.5 concentrations, with cross-validation coefficients of determination R2, RMSE and MAE values of 0.80, 11.50 µg/m3 and 8.13 µg/m3, respectively. PFM achieved the average results (R2 = 0.73, RMSE = 13.21 µg/m3 and MAE = 9.17 µg/m3), while the predicted results by ARIMA are comparatively poorer (R2 = 0.63, RMSE = 15.56 µg/m3 and MAE = 10.64 µg/m3) than RF and PFM. These findings reveal that the RF model is the most effective method for predicting PM2.5 and can be applied to other regions for new findings.
Consensus insertion/deletions and amino acid variations of all coding and noncoding regions of the SARS-CoV-2 Omicron clades, including the XBB and BQ.1 lineages
Authors: Soeharsono Hamong , Mahardika Bayu K. , Sudipa Putu H. , Sari Tri K. , Suardana Ida B. K. , Mahardika Gusti Ngurah 
Published: January 2023

Abstract
The currently dominant Omicron variant of the severe acute respiratory syndrome 2 (SARS-CoV-2) has swiftly diverged into clades. To predict the probable impact of clades, the consensus insertions/deletions (indels) and amino acid substitutions of the whole genome of clades were compared with original SARS-CoV-2. The indels and polymorphic amino acids were clade specific or shared among clades. The 21K clade has unique indels and substitutions, which probably represents reverted indels/substitutions. Three observed probable indirect evidences of SARS-CoV-2 attenuation in Omicron clades were deletion in Nucleocapsid, deletion in 3’-untranslated region, and truncation in open reading frame 8.
Structural and functional analysis of 31 HbMYB gene family members in rubber tree leaves
Authors: Liu Mingyang , Fan Songle , Yang Hong , Guo Bingbing , Dai Longjun , Wang Lifeng 
Published: January 2023

Abstract
The plant MYB transcription factor family participates in growth, differentiation, metabolism, and biotic and abiotic stress responses. The functions of HbMYBs in rubber tree leaves remain to be elucidated. In this study, molecular biology, bioinformatics and plant pathology techniques were used to identify and analyze HbMYB gene family members in rubber tree leaves. According to the results, 31 members of the HbMYB gene family were identified in the rubber tree. They were divided into 10 subgroups and were characterized by 3 unique motifs, in which 25 HbMYB members have R2R3-MYB conserved structural domains. Expression analysis revealed that the members of the HbMYB family were mainly expressed in flowers. The expression of the S22 and S20 subgroups increased significantly in powdery mildew-infected leaves. The expression of 10 HbMYBs was significantly induced by ethylene treatment. These results provide fundamental knowledge for further studying the response of the HbMYB family to biotic and abiotic stresses in rubber tree.
Longitudinal relationships among career adaptability, resilience, and career commitment in Chinese nursing undergraduates: Testing differences in career interest between cross-lagged models
Authors: Zhang Jingjing , Zhao chengjia , Li Feiyue , Wang Xiaoyi , Xu Huihui , Zhou Miaomiao , Huang Yiru , Yang Yeqin , Yu Guoliang , Zhang Guohua 
Published: January 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
A phosphorylation-deficient mutant of Sik3, a homolog of Sleepy, alters circadian sleep regulation by PDF neurons in Drosophila
Authors: Kobayashi Riho , Nakane Shin , Tomita Jun , Funato Hiromasa , Yanagisawa Masashi , Kume Kazuhiko 
Published: January 2023

Abstract
Sleep behavior has been observed from non-vertebrates to humans. Sleepymutation in mice resulted in a notable increase in sleep and was identified as an exon-skipping mutation of the salt-inducible kinase 3 (Sik3)gene, conserved among animals. The skipped exon contains a serine residue that is phosphorylated by protein kinase A. Overexpression of a mutant gene with the conversion of this serine into alanine (Sik3-SA) increased sleep in both mice and the fruit fly Drosophila melanogaster. However, the mechanism how Sik3-SA increases sleep remains unclear. Here, we found, in Drosophila, that Sik3-SA overexpression in all neurons increased sleep both under light-dark (LD) conditions and constant dark (DD) conditions, and its overexpression only in PDF neurons, which is the center of circadian clock, increased sleep during subjective daytime with decreasing the amplitude of circadian rhythm. Moreover, suppression of Sik3-SA overexpression only in PDF neurons in flies overexpressing Sik3-SA in all neurons reversed sleep increase during subjective daytime. In addition, Sik3-SA overexpression in all neurons did not affect the circadian rhythmicity of clock gene expression. These results indicate that Sik3-SA altered the circadian function of PDF neurons and resulted in the increase in sleep during subjective day under constant dark condition.