bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Treatment Of Colorectal Mucinous Adenocarcinoma Could Be Discriminate From Adenocarcinoma: Genesis And Immune Microenvironment Differences On Transcript Level
Authors: Liu Jianbo , Qiu Siyuan , Fu Xiaorui , Zhou Bin , Zu Ruijuan , Lv Zhaoying , Li Yuan , Yang Lie , Zhou Zongguang 
Published: August 2023

Abstract
Background
Mucinous adenocarcinoma (MC) of colorectal cancer (CRC) differs from adenocarcinoma (AD) in clinical features and molecular characteristics. Current treatment of colorectal MC isn't precise enough and the molecular characteristics remain unclear. The study aims to explore the difference between MC and AD of CRC on transcriptome level, for possibility of treating colorectal MC precisely.
Methods
We accessed the data of CRC patients from The Cancer Genome Atlas (TCGA) database, then we performed differential analysis and weighted gene co-expression network analysis (WGCNA) to identify the differential hub RNAs between colorectal MC and AD. Functional enrichment analysis, RNAs co-expression networks, risk score based on least absolute shrinkage and selection operator (LASSO) regression model and validation in Gene Expression Omnibus (GEO) database, survival analysis were also performed. Finally, differential hub lncRNAs and hub RNA of significant module were validated by quantitative real time PCR (qRT-PCR) among different colon cancer cell lines.
Results
In total, we found 1680 differential expressed RNAs (DERs) and 4 significant modules (darkred, magenta, lightstellblue1, tan) comparing colorectal MC (52, 13.3%) with AD (340, 86.7%). From the functional enrichment analysis and RNAs co-expression networks, the darkred module was considered as a mucin-associated module, while some others may be associated with unique immune progress. Construction of logistic regression model and calculation of risk score based on differential hub RNAs in darkred module showed acceptable result in both TCGA and GEO data. Survival analysis suggested that many differential hub RNAs were positive and correlated with better survival. Finally, 8 differential hub RNAs in the darkred module (CTD-2547H18.1, CTD-2589M5.4, RP11-234B24.2, LA16c-321D4.2, LINC00261, RP11-25K19.1, COLCA1 and CAPN9) were validated by qRT-PCR. Except for LA16c-321D4.2 and COLCA1, all other RNAs showed higher expression levels in mucin-producing colorectal cell lines (Ls174T, HT-29 and T84).
Conclusion
This study suggests that clinical treatments for colorectal MC should be differentiated from AD. Further exploration of enterocyte (goblet cell) differentiation with tumor genesis and the distinct immune progression of MC may help to identify key therapeutic targets for colorectal MC. Further research into the application of immunotherapy to colorectal MC is needed.
Spatial Resolution of Phosphenes within the Visual Field using Non-Invasive Transcranial Alternating Current Stimulation
Authors: Sadrzadeh-Afsharazar Faraz , Douplik Alexandre 
Published: August 2023

Abstract
Non-Invasive Transcranial Alternating Current Stimulation (NITACS) is a method that applies weak electrical currents to the scalp or face to modulate brain activity. A fascinating application of NITACS is the induction of phosphenes — visual phenomena where individuals perceive light without external stimuli. These phosphenes have been observed and generated through various techniques, including direct electrical stimulation of the visual cortex. However, NITACS provides a non-invasive way to create these visual effects. This research aimed to understand the spatial resolution of NITACS-induced phosphenes, vital for visual aid technology and neuroscience. Eight healthy participants underwent NITACS with a novel electrode configuration on the face. Findings indicated that NITACS could induce phosphenes that showed spatially defined patterns in the visual field. The phosphene locations differed among participants but were consistently within the visual field. These patterns remained stable across repeated stimulations. Optimal parameters were determined for inducing vibrant phosphenes without discomfort. The study also identified electrode positions that moved phosphenes to various visual field regions. Receiver Operating Characteristics (ROC) analysis estimated specificity and sensitivity at 70.7% and 73.9%, respectively, with a control trial effectiveness of 98.4%. Overall, NITACS holds promise as a reliable non-invasive means to modulate visual perception.
Inhibition of endoplasmic reticulum stress prevents renal pathology in hindlimb unloaded mice
Authors: Ranade Anu V , Ray Animikh , Khan Amir Ali , Gul Muhammad Tehsil , Jose Josemin , Ramachandran Gopika , Karim Asima , Ahmad Firdos , Ebby Suni , Qaisar Rizwan 
Published: August 2023

Abstract
Hindlimb unloaded (HU) mouse, an animal model of prolonged bed rest and spaceflight, exhibits extensive damage to several body organs. However, relevant effects on renal tissues are unknown. In this work effects of protein dysregulation by endoplasmic reticulum (ER), termed ER stress to renal histology and molecular phenotype in HU mice has been investigated. Role of 4-phenyl butyrate (4PBA) in reversal of these detrimental effects was also evaluated. c57BL/6j mice was categorized into three cohorts-ground-based controls, (GC),treated with vehicle (HU) , treated with 4PBA. It was observed that an HU-induced glomerular widening and an obliteration of Bowman’s space takes place in HU mice, which were partly reversed by 4PBA.
The effect of protein dysregulation by endoplasmic reticulum in HU mice and its reversal was evaluated at transcriptome level using RNA-Seq analysis using an illumina next generation sequencing platform. Downstream analysis of mapped transcriptome provides support to the hypothesis that PBA has the potential to reverse renal stress in HU mice. The GO enrichment analysis validated heightened ER stress in HU kidneys and its reversal with 4PBA treatment. These findings were supported by KEGG-based and Reactome-based pathway analysis. 4PBA treatment upregulated PPAR signaling pathway, which protects against ER stress.
A machine learning-guided strategy for replacement of peripheral venous catheters in the prevention of hospital-acquired bacteremia: A comparative cost-effectiveness analysis
Authors: Jakobsen Rune , Nielsen Thomas , Christensen Jakob , Leutscher Peter , Sørensen Sabrina , Koch Kristoffer 
Published: August 2023

Abstract
Machine learning (ML) models for early identification of patients at risk of hospital-acquired bacteremia (HAB) may guide a preventive strategy of routine replacement of peripheral venous catheters (PVCs). The feasibility and cost-effectiveness of such an approach have not been evaluated.

In this study, we developed ML models for the preventive strategy of ML-guided routine replacement of PVCs and evaluated this strategy according to number needed to harm and incremental cost-effectiveness for preventing a HAB-related death. The ML-guided strategy of early identification of patients at risk of HAB can lead to a reduction in the labor-intensive intervention of routine PVC replacement. However, this is at the expense of increased costs for establishing and maintaining ML model guidance, as well as increased costs for patients erroneously categorized as low-risk for HAB by the ML model.
Atrial fibrillation may increase the risk of osteoporotic fracture in Asian population: A systematic review and meta-analysis
Authors: Chen Wei , Zhang Yuyu , Guo Haiyi , Li Tingting , Yin Zhuohao , Zhao Kai , Chen Long , Ge Wei 
Published: August 2023

Abstract
Objectives

Falls among the elderly often occur in the community. The elderly with osteoporosis frequently induces fractures after falls. The incidence of osteoporotic fractures is high, and the prognosis is poor, which warrants attention. This is particularly crucial for community primary health care providers, as they need to identify the risk factors contributing to increased osteoporotic fractures. Several cardiovascular diseases have been linked to an elevated risk of bone fractures. However, it remains unclear whether atrial fibrillation increases the risk of bone fracture. To address this, we conducted a systematic review and meta-analysis of relevant studies to quantify the magnitude of the association between atrial fibrillation and the incidence of osteoporotic fractures.

Methods

PubMed/MEDLINE, EMBASE and Cochrane Library were searched for relevant studies from January 1st 2000 to March 10th 2022. Observational studies which compared the association of osteoporotic fracture in atrial fibrillation group with non-atrial fibrillation group were included.

Results

A total of 5 cohort studies with 187868 participants met all the eligibility criteria for our study. 835 people suffered a fracture in atrial fibrillation group and 6512 in non-atrial fibrillation group. The overall risk of osteoporotic fractures was non-statistically higher in patients with 5.4% (835/15395) in atrial fibrillation group and 3.8% (6512/172473) in non-atrial fibrillation group. Analysis of included studies observed non-significant association between atrial fibrillation and osteoporotic fractures (odds ratio (OR)=1.17, 95% confidence intervals (CIs)=0.60-2.29). However, subgroup analysis displayed that Asian population with atrial fibrillation had a higher risk of fracture (OR=1.61, 95%CI=1.38-1.87), whereas Caucasian population with atrial fibrillation suggested a negative result. (OR=0.94, 95%CI=0.24-3.59).

Conclusions

The association between atrial fibrillation and an increased risk of fracture may be related to race. Asians with atrial fibrillation have been found to be more prone to fractures, whereas the risk remains insignificant in the Caucasian population. It is important to note that the number of studies included in this article is limited. Therefore, further research and additional data are needed to fully support the results of our study. Keywords
atrial fibrillation; osteoporotic fracture; risk factor; cohort study; meta-analysis
Stream quality and benthic macroinvertebrate diversity in the badeye area logone occidental province, southwestern chad.
Authors: Beosso Théophile Djikoloum 
Published: August 2023

Abstract
The aim of this study of benthic macroinvertebrates in the south-western Canton Badeye in Chad was to determine their population structure in relation to the physicochemical quality of the water in the Man baptem tributary. A total of 4012 benthic macroinvertebrates in 38 families were identified and counted. Arthropods were the most diverse, with 35 families, and the most abundant (92.32%) of the total, followed by Molluscs (6.65%) and Annelids (1.02%). Insects predominated at all sampling stations (89.40%), followed by Gastropoda (6.75%), Malacostraca (2.85%), Achaete (0.57%) and Oligochaete (0.45%). The preponderance of the Chironomidae, Psychodidae and Physidae families in station A3 implies that the water at this station is of poor quality compared with the other 3 stations. The Shannon-Weaver and Piélou indices are higher at station C3, confirming some of the physicochemical parameter values at this station. Overall, the 4 sampling stations are diverse. The abundance of certain benthic groups such as Chironomidae Diptera in the Man baptem tributary shows that this watercourse is subject to anthropogenic disturbance.
RAES：a rapid and high-fidelity method for artificial extrachromosomal circular DNA synthesis and its application as a bacterial DNA-free vector for robust microRNA overexpression
Authors: Xi Xiang , Jiaying Yu , Haoran Zhang , Han Peng 
Published: August 2023

Abstract
Extrachromosomal circular DNA (eccDNA) has recently gained increasing attention due to its significant role in cancer and other pathological states. The majority of circular DNAs detected by Circle-seq are small-size eccDNAs with enigmatic functions. One major technical hurdle is to synthesize eccDNA for functional assessment. Here, we describe RAES (RCA-product dependent Artificial EccDNA Synthesis), a rapid and economic method for artificial eccDNA synthesis. Eight eccDNAs carrying different microRNA genes (eccMIR) found in gastric cancer tissues, ranging from 329 bp to 2 189 bp in size, were created utilizing the RAES method. Exonuclease V and single restriction-endonuclease digestion identified the circular structure of synthetic eccDNAs. The DNA circularization efficiency afforded by RAES ranged from 15.6% to 31.1%, which was negatively correlated with the eccDNA length. In addition, we demonstrated that RAES-synthesized eccMIRs can express both miRNA-3p and -5p molecules efficiently in different cell types, independent of a canonical promoter. Further assays proved that these eccMIRs were functional as they were able to repress the luciferase gene containing a miRNA-target sequence in the 3’UTR as well as the endogenous mRNA targets. Together, RAES is an economic and efficient method to synthesize artificial eccDNAs, which also provides a promising way to produce bacterial DNA-free vectors for safe and robust miRNA overexpression in either fundamental research or clinical therapeutics.
Responses of plant biomass allocation and carbon storage characteristics to altitude gradient in alpine peat bogs
Authors: Kang Man-Ping , Zhao CZ , Ma Min 
Published: August 2023

Abstract
The change in hydrothermal conditions caused by altitude gradient will affect plant growth. The study of plant biomass and carbon storage in peat bogs at different elevations is of great significance for further understanding plant tolerance to habitat stress and the uncertainty of plant carbon sinks. According to the distribution characteristics of peat bogs in Zoige Plateau, 3400–3800 m peat bogs in the Shouqu Nature Reserve of the Yellow River were selected as experimental samples. The characteristics of plant biomass allocation, carbon storage, and their main influencing factors were studied by single-factor analysis and path analtysis. The results showed that (1) The biomass distribution ratio of plants in peat bogs was root > leaf > stem, and the subsurface biomass of vegetation was higher than that of above-ground biomass. With the increase in altitude, the aboveground biomass decreased, the total biomass and underground biomass increased first and then decreased, and the root-shoot ratio increased. (2) The organic carbon content of plants in peat bogs was higher in stem > leave > root. The underground carbon storage of plants was higher than that of above-ground carbon storage, and the total carbon storage decreased with the increase in altitude. (3) Path analysis showed that AD, SWC, and TS had direct positive effects on plant biomass and carbon storage, while DEP and pH had direct negative effects on plant biomass and carbon storage. The biomass allocation patterns and carbon storage characteristics of plants in peat bogs reflect the adaptation rules of plants in heterogeneous habitats. It is of great theoretical and practical value to understand the environmental response mechanism of plants in peat bogs under the global climate background and to evaluate wetland plants carbon sink.
Feasibility of Self-measurement Telemonitoring Using a Handheld Heart Sound Recorder in Patients with Heart Failure – SELPH multicenter pilot study
Authors: Kagiyama Nobuyuki , Kasai Takatoshi , Murata Nobutaka , Yamakawa Nobuhide , Tanaka Yuki , Hiki Masaru , Inoue Kenji , Sato Akihiro , Ishiwata Sayaki , Murata Azusa , Shitara Jun , Kato Takao , Suda Shoko , Matsue Yuya , Naito Ryo , Minamino Tohru , Yanagisawa Naotake , Daida Hiroyuki 
Published: August 2023

Abstract

Introduction:
Multi-parametric assessment, including heart sounds in addition to conventional parameters, may enhance the efficacy of noninvasive telemonitoring for heart failure (HF). We sought to assess the feasibility of self-telemonitoring with multiple devices including a handheld heart sound recorder and its association with clinical events in patients with HF.
Methods
Ambulatory HF patients recorded their own heart sounds, mono-lead electrocardiograms, oxygen saturation, body weight, and vital signs using multiple devices every morning for six months.
Results
In the 77 patients enrolled (63 ± 13 years old, 84% male), daily measurements were feasible with a self-measurement rate of > 70% of days in 75% of patients. Younger age and higher Minnesota Living with Heart Failure Questionnaire scores were independently associated with lower adherence (P = 0.001 and 0.017, respectively). A usability questionnaire showed that 87% of patients felt self-telemonitoring was helpful, and 96% could use the devices without routine cohabitant support. Six patients experienced 10 HF events of re-hospitalization and/or unplanned hospital visits due to HF. In patients who experienced HF events, a significant increase in heart rate and diastolic blood pressure and a decrease in the time interval from Q wave onset to the second heart sound were observed 7 days before the events compared with those without HF events.
Conclusions
Self-telemonitoring with multiple devices including a handheld heart sound recorder was feasible even in elderly patients with HF. This intervention may confer a sense of relief to patients and enable monitoring of physiological parameters that could be valuable in detecting the deterioration of HF.
Genetic Diversity, Correlation and Genotype by Yield by Trait (GYT) Analysis of Grain Yield and Nutritional Quality Traits in Sorghum (Sorghum bicolor [L.] Moench) Genotypes in Tigray, northern Ethiopia
Authors: Welderufael Shushay , Abay Fetein , Ayana Amsalu , Amede Tilahun 
Published: August 2023

Abstract
Selecting sorghum genotypes with higher grain yield and nutritional quality is essential to tackle food insecurity and malnutrition in arid and semi-arid areas. Therefore, this study aimed to determine the genetic diversity, trait association and genotype by yield by trait (GYT) analysis and to select superior sorghum genotypes. One hundred and ten sorghum genotypes were evaluated at three locations in Tigray during the 2018 and 2019 growing seasons using alpha lattice design. Traits such as grain yield, protein content, ash content, starch content, zinc content, iron content, calcium content and magnesium content were profiled. Results showed that wide range and highly significant (p < 0.001) genotype mean performance in each environment as well as combined environments. Several highly performing genotypes were distinguished for each trait studied that could be exploited as breeding parents or direct use. This study further detected highly significant variation (p < 0.001) among the test genotypes for all the traits studied in individual environments and across environments suggesting the presence of sufficient genetic diversity for selection. The high broad-sense heritability (H2 > 0.9) in all individual environments and moderate to high (H2 > 0.0.41 < 0.82) in pooled environments recorded in the present study assured the possibility of effective selection among the genotypes. Besides, strong positive and negative associations were detected between some of the traits in individual and across environments. The significant positive association between traits indicates that both the traits can be improved concurrently through direct selection. Using the GYT analysis, we suggest ten promising sorghum genotypes for direct use or breeding programs in arid and semi-arid areas in general and in Tigray in particular.
FDG PET/CT, Precise Positioning of the Criminal Focus in the Osteogenic Region of Breast Cancer Bone Metastases after Therapy
Authors: Lin Runlong , Lv Huiyun , Yu Jing , Tian Aijuan , Song Chen 
Published: August 2023

Abstract
Purpose
The present study endeavours to investigate the utility and indispensability of FDG PET/CT in appraising the post-treatment efficacy of bone metastasis in cases of breast cancer.
Method
A cohort of 11 patients diagnosed with breast cancer and suffering from bone metastases was enrolled for this investigation. These patients underwent repeated FDG PET/CT evaluations, with comprehensive clinical records and sufficient follow-up duration available. Division of patients and bone metastases ensued into three distinct groups - amelioration, stability, and progression - predicated upon the response exhibited by each bone metastatic site subsequent to treatment. The ensuing analysis and juxtaposition encompassed PET and CT image alterations, levels of serum tumour biomarkers (CEA, CA153), as well as biochemical indicators (ALP, Ca), within each group across the identical time frame. Furthermore, the inquiry scrutinized disparities between immunohistochemical outcomes of primary breast cancer and bone metastases displaying escalated FDG uptake as discerned through PET/CT post-treatment.
Results
Discernible disparities in serological indices (CEA, CA153, ALP, and Ca) were absent among patients classified under distinct efficacy categories (p > 0.05). Nonetheless, diminished expression of Her-2 engendered an elevated likelihood of suboptimal efficacy (p < 0.05). Appraisal of efficacy, guided by individual bone metastases, unveiled notable fluctuations in FDG uptake through PET (SUVmax) amid the three groups (p < 0.05), while density variations in CT scans did not reach statistical significance (p = 0.243). Variances surfaced in FDG uptake, alterations in CT density, and levels of CEA and CA153 within patient sera before and after treatment (p < 0.05). Of these indicators, paramount diagnostic efficacy was ascribed to FDG PET metrics: alterations in FDG uptake (AUC 0.972) and post-therapy SUVmax (AUC 0.949). Immunohistochemical examination of bone metastases within the progressive group diverged from primary lesions.
Conclusions
FDG PET/CT confers precise assessment of the post-treatment efficacy pertaining to each bone metastatic site in breast cancer cases. The modality facilitates identification of eluding foci following extant therapies, localization for pathological assessment, and bears substantive significance in evaluating therapeutic efficacy, refining treatment stratagems, and prognosticating the trajectory for breast cancer patients contending with bone metastases.
Hemodynamics of proximal coronary lesions in patients undergoing transcatheter aortic valve implantation: Patient-specific in silico study
Authors: BELLOUCHE Yahia , ABDELLI Sirine , HANNACHI Sinda , BENIC Clement , VEN Florent LE , MANSOURATI Jacques , DIDIER Romain 
Published: August 2023

Abstract

Aortic stenosis (AS) is a prevalent disease with significant implications for patients' prognosis, particularly when accompanied by coronary artery disease. However, the optimal revascularization strategy remains uncertain, especially with the emergence of transcatheter valve implantation (TAVI). In this study, we aimed to investigate the hemodynamics of proximal coronary lesions in the context of AS and their response to aortic valve replacement (AVR) using computational fluid dynamics (CFD) based on patient-specific data. Patient-specific CT scans and invasive coronary angiograms were utilized to create 3D models of the coronary arteries and aortic valve, enabling CFD simulations to provide pressure, flow, and shear stress data. The results demonstrated excellent agreement between CFD-estimated fractional flow reserve (FFR) and invasive measurements, affirming the accuracy of CFD methods.No noticeable variation of iFR (0.901 vs. 0.901, p = 0.89), CFR (1.97 vs. 1.98,p=0.49) nor FFR (0.805 vs. 0.804, p=0.81) values post-procedure were found. Additionally, AVR led to a reduction in the areas of coronary arteries exposed to high shear stress. These findings suggest that a post TAVI revascularization approach to non-critical proximal lesions (<70\%) may be deemed safe. This study underscores the potential benefits of CFD in preoperative planning for patients with AS and concomitant coronary artery disease, offering valuable insights into coronary physiology and the hemodynamic impact of TAVI.