bluuebunny/crossref_metadata_2025_split huggingface dataset:
Landmark Base Point Approach to Positional Accuracy Analysis for Historical Geographic Map Applications Before WWI: A case study 1914 German Blue and 1836 Moltke about in Historical Peninsula of Istanbul
Authors: Sahin Cumhur , Ergun Bahadir , Bilucan Furkan 
Published: May 2023

Abstract

Thanks to advances in current technology and computer software, mathematical computations and engineering applications that used to require hours or even days can now be performed quickly. Cartography, unquestionably one of the world's oldest branches of scientific study, is among the fields that have been most influenced by technological improvements. These improvements have enhanced interest in historical maps while also paving the way for modern mapping. All of this process has created new topics of study for cartographers. Also, it is possible to examine old maps using the Map Analyst software which can enable the investigation of time dependent changes. Helmert, Affine and Huber loss function as robust estimation method were analysed. Thanks to the distortion grids, it can be observed in which region the movement is denser. In this study, by using 29 absolute tie points, the old Istanbul maps (the German Blue maps produced in 1913-1914 and the Moltke maps produced in 1836-1837) were compared with the new maps. Additionally, it is aimed to observe the change in the field of cartography with the opportunities offered by the latest software and to provide support to reliable data. It has been observed that a comparison can be made by using the points on the historical maps of Istanbul with the corresponding points on a new contemporary map.
Comprehensive Analysis of Immune Implication and Prognostic Value of CCDC137 in Hepatocellular Carcinoma
Authors: Ho Mei-Yi , Hong Qing-Ting , Bao Ting-Yu , Shen Hao-Ran , Chen Ji-Chun , Guo Xu‑Guang 
Published: May 2023

Abstract
Hepatocellular carcinoma (HCC) is the most predominant pathological type of primary liver cancer and is a serious threat to people's lives and health worldwide. In this study, the Cancer Genome Atlas (TCGA) database was used to explore the immunological role of CCDC137 in hepatocellular carcinoma and its prognostic value, and a predictive model containing CCDC137-related immune modulators was developed using Cox regression analysis. Levels of CCDC137 were significantly higher in liver tissue of HCC patients than in normal tissue, and CCDC137 expression was also significantly correlated with pathological stage, tumor stage, and the expression of CCDC137 was also significantly correlated with pathological stage, tumor stage, and histological grade. The robustness of the predictive ability of the model was fully validated by plotting ROC curves and nomograms and by the external validation cohort of the International Cancer Genome Consortium (ICGC) database. Our model provides additional options for targeted, immune-based systemic therapy.
Efficacy and executive function of solution focused brief therapy on adolescent depression：A study of prefrontal hemodynamic functions using fNIRS
Authors: Chen Haisi , Zhou Mengmeng , Luo Hong , Yu Zhenghe 
Published: May 2023

Abstract
To explore the efficacy and executive function of solution focused brief therapy (SFBT) on adolescent major depressive disorder (MDD). A total of 129 adolescents with MDD were recruited, of whom 28 received solution-focused brief therapy (SFBT group) and 25 received pcychodynamic psychotherapy (AC group). Executive function, depressive and anxiety symptoms were assessed at baseline (T0), at the time of the third intervention (T3), the sixth intervention (T6), and the 10th intervention (T10). After the third intervention, the scores of PHQ-9 and GAD-7 of the participants in the SFBT group decreased significantly, which had the cumulative effect at the 6th and 10th interventions. The verbal fluency task (VFT) performances of the SFBT group participants yielded significantly higher scores after the third intervention and remained increasing at the 6th and 10th interventions. The AC group steadily decreased after the intervention. fNIRS data showed that the mean oxy-Hb values of channels 3, 11 and 12 were gradually significantly increased in the SFBT group than the AC group after 10th interventions. SFBT might improve depressive and anxiety symptoms as well as executive function of adolescent depression.
Rhodotorula mucilaginosa YR29 is able to accumulate Pb2+ in vacuoles: a yeast with bioremediation potential
Authors: de Paz Gabriela Angeles , Gutiérrez Hugo Martínez , Granillo Adrián Ramírez , Villegas Edgar Oliver López , Medina-Canales María Gabriela , Rodríguez-Tovar Aida Verónica 
Published: May 2023

Abstract

In response to pollution, microorganisms showed unique mechanisms to resist and detoxify harmful metals. This study shows the relationship between presence of heavy metals and plant growth regulator compounds. Additionally, the responses of Rhodotorula mucilaginosa YR29 isolated from the rhizosphere of Prosopis sp. growing in a polluted mine jal in Mexico are presented. This research carries out a phenotypic characterization of R. mucilaginosa to identify response mechanisms to metals and confirm its potential as a bioremediation agent. Firstly, Plant Growth-Promoting (PGP) compounds were assayed using the Chrome Azurol S (CAS) medium and the Salkowski method. Then, to clarify its heavy metal tolerance mechanisms, several techniques were performed, such as optical microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) supplemented with assorted detectors. Likewise, scanning transmission electron microscopy (STEM) was used for elementary mapping of the cell. Further, yeast viability after all treatments was confirmed by confocal laser scanning microscopy (CLSM). The results have suggested that R. mucilaginosa could be a PGP yeast capable of triggering Pb2+ biosorption (representing 22.93% of the total cell surface area, the heavy metal is encapsulated between the cell wall and the microcapsule), and Pb2+ bioaccumulation (representing 11% of the total weight located in the vacuole). Based on these results, R. mucilaginosa as a bioremediation agent and its wide range of useful mechanisms for ecological purposes are highlighted.
Clinical predictive value of methylated SEPT9 combined with NLR, PLR and LMR in colorectal cancer
Authors: Yan Bokang , Huang Meiyuan , Deng Shuang , Yang Zhenyu , Deng Yi , Chen Dongliang 
Published: May 2023

Abstract
Purpose This study aimed to investigate clinical predictive values of methylated septin9 (mSEPT9) combined with neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and lymphocyte-to-monocyte ratio (LMR) in colorectal cancer (CRC).Methods 329 subjects composed of 114 CRC patients, 105 polyps patients and 104 healthy participants were prospectively recruited. Clinicopathologic features were collected and analyzed. Plasma samples were collected for mSEPT9, NLR, PLR and LMR test. The sensitivity, specificity and area under the curve (AUC) of each biomarker separately or in combination were estimated by the receiver operating characteristic (ROC) curve.Results The levels of NLR and PLR and the positive detection rate (PDR) of mSEPT9 in CRC patients were obviously higher than non-CRC subjects, while LMR was the opposite. The PDR of mSEPT9 in CRC patients was significantly correlated with age, tumor size, tumor stage and M stage. ROC curve analysis demonstrated moderate diagnostic values of mSEPT9, NLR, PLR and LMR in CRC patients with AUC of 0.78 (Se = 0.68, and Sp = 0.89), 0.78 (Se = 0.68, and Sp = 0.83), 0.80 (Se = 0.68, and Sp = 0.81), and 0.77 (Se = 0.72, and Sp = 0.73), respectively. Combination of these four biomarkers dramatically enhanced the diagnostic accuracy of CRC (AUC = 0.92, Se = 0.90, and Sp = 0.87), especially CRC patients with large tumors (AUC = 0.95) or distal metastasis (AUC = 0.95).Conclusion mSEPT9, NLR, PLR and LMR showed the potential to be reliable biomarkers for the diagnosis of CRC. Combination of all together further improved the diagnostic accuracy of CRC.
Long-wave opsin involved in body color plastic development in the brown planthopper
Authors: Lu Jia-Bao , Li Ze-Dong , Ye Zhuang-Xin , Huang Hai-Jian , Chen Jian-Ping , Li Jun-Min , Zhang Chuan-Xi 
Published: May 2023

Abstract
Background Opsin is an important molecular basis of the visual system, besides, it was discovered that opsins have also evolved additional functions despite color vision. However, the study of the unconventional function has been limited to a few species. With the increasing number of genome databases, various numbers and types of opsins have been identified in insects due to gene duplications or losses. The brown planthopper (Nilaparvata lugens, BPH) is a Hemipteran rice pest known for its long-distance migration capability. Opsins in BPH were identified and characterized by analyzing the genome and transcriptome. RNA interference (RNAi) was used to investigate the functions of opsins, and the potential molecular mechanism was studied by the Illumina Novaseq 6000 platform-based transcriptome sequencing.Results Four opsins belonging to G protein-coupled receptors were identified in BPH genome, including one long-sensitive opsin (Nllw) and three ultraviolet-sensitive opsins (NlUV1-3). A tandem array of NlUV1/2 on chromosome suggested a gene duplication event, with similar exons distribution. Spatiotemporal expression modes showed that the four opsins were highly expressed in eye with age-different expression levels, indicating functional differentiation. RNAi targeting each of the four opsins did not significantly affect the survival of BPH in phytotron, but silencing of Nllw resulted in melanism of the body color. Further transcriptome analysis revealed up-regulation of a tyrosine hydroxylase gene (NlTH) and down-regulation of an arylalkylamine-N-acetyltransferases gene (NlaaNAT) in BPH, indicating that Nllw is involved in body color plastic development through the tyrosine-mediated melanism pathway.Conclusions This study provides the first evidence in a Hemipteran insect that an opsin (Nllw) takes part in the regulation of cuticle melanization, confirming the visual system cross-talks with the morphological differentiation in insects.
Interest in using patient portals among adolescents in mental health care - a cross-sectional study
Authors: Nielsen Martine Stecher , Steinsbekk Aslak , Nøst Torunn Hatlen 
Published: May 2023

Abstract
Introduction: Adolescents in mental healthcare may benefit from using patient portals to access personalized information about their health and treatment. While no studies consider the interest in using patient portals among adolescents in mental health care, some factors have been found to be associated with interest in and use of patient portals in other patient groups. The aim was therefore to explore the associations between interest in using patient portals and patient activation, self-reported health, depressive symptoms, diagnosis, healthcare utilization and eHealth literacy among adolescents in mental health care.Methods A cross-sectional study among adolescents between 12 and 18 years of age receiving or having received treatment at four different specialist child and adolescent mental healthcare services across Norway. The adolescents´ answers to the questionnaire were linked to data on their healthcare utilization and ICD-10 diagnoses from the Norwegian Patient Registry. Data were analysed with descriptive statistics and bivariate tests.Results The 53 adolescents who participated, had a mean age of 15 years and 68% of them identified as female. Two out of three (64%) were interested in using patient portals. Most of the factors were not associated with interest in using patient portals, but adolescents with mental and behavioural disorders (F diagnoses, 75% interested) were more interested in using patient portals than those with symptoms and signs involving cognition, perception, emotional state, and behaviour (R diagnoses, 31% interested).Conclusion Except for diagnosis, this study did not identify any specific factor that is likely to have an impact on patient portal interest among adolescents in specialist mental health care.
Sox17 Protects Human Brain Microvascular Endothelial Cells from AngII-induced Injury by Regulating Autophagy and Apoptosis
Authors: Wang Yanyan , Fang Marong , Ren Qiannan , Qi Wei , Bai Xinli , Amin Nashwa , Zhang Xiangjian , Li Zhenzhong , Zhang Lihong 
Published: May 2023

Abstract
Intracranial aneurysm (IA), is a localized dilation of the intracranial arteries, the rupture of which is catastrophic. Hypertension is major IA risk factor that mediates endothelial cell damage. Sox17 is highly expressed in intracranial vascular endothelial cells, and GWAS studies indicate that its genetic alteration is one of the major genetic risk factors for IA. Vascular endothelial cell injury plays a vital role in the pathogenesis of IA. The genetic ablation of Sox17 plus hypertension induced by AngII can lead to an increased incidence of intracranial aneurysms had tested in the Previous animal experiments. In order to study the underlying molecular mechanisms, we established stable Sox17-overexpressing and knockdown cell lines in human brain microvascular endothelial cells (HBMECs) first. Then flow cytometry, western blotting, and immunofluorescence were employed. We found that the knockdown of Sox17 could worsen the apoptosis and autophagy of HBMECs caused by AngII, while overexpression of Sox17 had the opposite effect. Transmission electron microscopy displayed increased autophagosomes after the knockdown of Sox17 in HBMECs. The RNA sequencing analysis shown that dysregulation of the Sox17 gene was closely associated with the autophagy-related pathways. Our study suggests that Sox17 could protect HBMECs from AngII-induced injury by regulating autophagy and apoptosis.
Pain Control and Opioid Consumption Following Laparoscopic Appendectomy with the Use of Quadratus Lumborum Regional Anesthesia
Authors: Pace Devon , Mack Shale J. , Sadacharam Kesavan , Lang Robert S. , Burke Brian , Fishlock Keith , Berman Loren 
Published: May 2023

Abstract
Purpose
This study analyzes the implementation of the routine use of quadratus lumborum blocks (QLBs) on postoperative pain and opioid consumption among children undergoing laparoscopic appendectomy compared to those not receiving regional anesthesia.
Methods
Children undergoing laparoscopic appendectomy within a multi-hospital children’s healthcare system were retrospectively reviewed from 2017 through 2021. Patients were stratified by appendicitis type (uncomplicated vs. complicated). Pain scores and opioid consumption in the post-anesthesia care unit (PACU) and within the first 24 hours postoperatively were compared by block status (no block [NB] vs. QLB) and appendicitis type.
Results
2033 patients were reviewed, and 610 received a QLB. The frequency of rescue opioid use was reduced in the PACU (uncomplicated: QLB 46.6% vs. NB 54.6%, p = 0.005; complicated: QLB 28.5% vs. NB 39.9%, p = 0.01) and postoperatively (complicated: QLB 33.7% vs. NB 52.9%, p < 0.001) for those who received a QLB. This resulted in reduced opioid consumption as measured by morphine milligram equivalents per kilogram postoperatively.
Conclusion
QLBs can be safely administered in children and provide improvements in opioid consumption postoperatively. QLBs should remain a strongly favored regional anesthetic technique because of their wide applicability for abdominal surgeries in order to minimize rescue opioid analgesic use.
Level of Evidence: Level III
Death of a palaeochannel: slow abandonment of an avulsed channel on the Riverine Plains, SE Australia
Authors: Kemp Justine , Pietsch Timothy 
Published: May 2023

Abstract

Avulsion of rivers can be a gradual process that is associated with a metamorphosis of channel pattern or changed channel characteristics. The processes controlling avulsion, and hence anastomosis, often operate too slowly to study by measuring active river systems, and hence well preserved Late Quaternary rivers offer one of the best ways to study the long-term development of avulsive systems. The modern and ancient channels of the Murrumbidgee River provide a classic example of long-lived, semi-static anastomosis, operating on timescales that include stadial and interstadial climate cycles. Over the last glacial cycle, regional avulsions have occurred every ~ 12 ka while maintaining an anastomosing pattern through the slow infill of abandoned channels. The Yanco Creek Palaeochannel System emerged from a period of high discharge linked to snowmelt in the terminal Pleistocene. Here, geomorphological mapping of the Yanco System was conducted together with single-grain, optically stimulated luminescence dating of sediments in the channel belt. Since the main phase of channel construction during the Last Glacial Maximum, the Yanco system has functioned as a flood conduit and minor anabranch of the Murrumbidgee River, with reworking of ancient channel sediments by an underfit stream that is ongoing to the present day. Our new ages of ~ 13–14 ka are interpreted as channel sedimentation during an underfit phase following avulsion. The prevalence of full and partial avulsion in this environment may be complicating palaeohydrological interpretations of ages for channel activity, and reworking has gone unrecognised. We contend that some previous interpretations of the significance of ages for sediments collected from Riverine Plain palaeochannels may need revision.
Predicting effects of non-point source Pollution emission control schemes Prediction Based on VMD-BiLSTM and MIKE21
Authors: Zhang Xianqi , Qi Yu , Liu Fang , LI Haiyang , Sun Shifeng 
Published: May 2023

Abstract
Controlling non-point source (NPS) pollution is crucial for implementing water environment management, and simulating the water quality response to NPS pollution emission control schemes is of great importance. Variational mode decomposition (VMD) can overcome endpoint effects and modal aliasing issues, effectively separating intrinsic mode components. Bidirectional long short-term memory (BiLSTM) can fully mine the information contained in time series and has good predictive performance. MIKE21, when coupled with the Ecolab module, can well simulate the diffusion process of NPS pollution. The Weihe River water environment prediction model was constructed using VMD-BiLSTM and MIKE21, with ammonia nitrogen (NH3-N), total phosphorus (TP), and chemical oxygen demand (COD) as pollution indicators, showing the water quality response of the Weihe River within a few years after the implementation of agricultural and urban NPS pollution emission control schemes. Among them, the COD concentration decreased by up to 71.3%, the NH3-N concentration decreased by up to 31.4%, and the TP concentration decreased by up to 43.1%. The results show that the water quality of the Weihe River can be significantly improved by controlling NPS pollution emission, and reducing agricultural NPS pollution emission is key to decreasing ammonia nitrogen and total phosphorus concentrations and improving water quality.
Identification a unique disulfidptosis classification regarding prognosis and immune landscapes in thyroid carcinoma and providing therapeutic strategies
Authors: Feng Zhanrong , Zhao Qian , Ding Ying , Xu Yue , Sun Xiaoxiao , Chen Qiang , Zhang Yang , Miao Juan , Zhu jingjing 
Published: May 2023

Abstract
Background Thyroid carcinoma (THCA) is a common type of cancer worldwide, and its incidence has been increasing in recent years. Disulfidoptosis, a recently defined form of metabolic-related regulated cell death (RCD), has been shown to play a sophisticated role in antitumor immunity. However, its mechanisms and functions are still poorly understood and the association between disulfidoptosis and the prognosis of patients with papillary thyroid carcinoma remains to be elucidated. This study aims to investigate the connection between disulfidoptosis and the prognosis of thyroid cancer, while also developing a prognostic index based on disulfidoptosis genes.Materials and methods We utilized 24 genes associated with disulfidoptosis to create the classification and model. To gather data, we sourced gene expression profiles, somatic mutation information, copy number variation data, and corresponding clinical data from the TCGA database for patients with thyroid cancer. Additionally, we obtained single-cell transcriptome data GSE184362 from the Gene Expression Omnibus (GEO) database for further analysis.Results In this study, we utilized 24 genes associated with disulfidoptosis to identify two distinct groups with different biological processes using non-negative matrix factorization (NMF). Our findings showed that Cluster 1 is associated with chemokines, interleukins, interferons, checkpoint genes, and other important components of the immune microenvironment. Moreover, cluster 1 patients with high IPS scores may be more sensitive to immunotherapy. We also provide drug therapeutic strategies for each cluster patients based on the IC50 of each drug. The Enet model was chosen as the optimal model with the highest C-index and showed that patients with high risk had a worse prognosis and weak cell-to-cell interactions in THCA. Finally, we established a nomogram model based on multivariable cox and logistic regression analyses to predict the overall survival of THCA patients.Conclusion This research provides new insight into the impact of disulfidoptosis on THCA. Through a thorough examination of disulfidoptosis, a new classification system has been developed that can effectively predict the clinical prognosis and drug sensitivity of THCA patients.
Domain analysis and site-directed mutagenesis of a thermophilic pullulanase from Thermotoga maritima MSB8
Authors: Zhao Yujie , Liu Yang , Fu Qiao , Zhou Ying , Qin Rui , Xiong Hairong , Wang Yawei 
Published: May 2023

Abstract
The structure of Thermotoga maritima MSB8 pullulanase (PulA) was modified for checking the relationship of domain and site-directed mutagenesis with enzymatic properties. PulA was roughly linked by five domains as CBM41-X-CBM48-catalytic domain-C domain from N-terminal to C-terminal. The deleted certain domains mutants as PulA1 (deleted CBM41) and PulA2 (deleted CBM41-X) were survived with different properties, whereas the PulA3 (deleted CBM41-X-CBM48) and PulA4 (deleted C domain) lose their enzyme activities. The apparent melting temperatures (Tmapp) measured by differential scanning fluorimetry of PulA, PulA1 and PulA2 were 76.5 oC, 78.8 oC and 66.6 oC, respectively. The thermal stability of PulA1 was increased slightly but PulA2 was reduced significantly, which shows that the existence of X domain may play a positive role for stability of PulA. To investigate the interaction of CBM48 with X domain, a few site-directed mutants as M1 (E172G), M2 (E172G/R190G), M3 (E172G/D198G), M4 (E172G/R190G/D198G) on the surface of X domain were constructed to reduce the hydrogen bond between X domain with CBM48. The optimum temperature of those four mutants and PulA were 90 oC. The Tmapp values of M1, M2, M3, and M4 were 77.4 oC, 71.7 oC, 73.2 oC and 71.9 oC, respectively. The specific activity of M1 and M2 were increased slightly, while the pH stability of M3 and M4 were significantly improved at low pH. This study provides the information of the structure-function relationship of pullulanase.