bluuebunny/crossref_metadata_2025_split huggingface dataset:
Search for polymorphic variants of candidate genes contributing to individual radiosensitivity
Authors: Isubakova D. S. , Litviakov N. V. , Tsymbal O. S. , Usova T. V. , Tsyplenkova M. Yu. , Milto I. V. , Takhauov R. M. 
Published: January 2023

Background.Cytogenetic damage (СD) in lymphocytes induced by low doses (up to 0.1 Sv) of ionizing radiation (IR) is the main cytogenetic sign of individual radiosensitivity of the human body. In addition to DNA repair and cell death, which affect the formation of СD and its elimination, IR effects on the cell can be manifested through changes in proliferation of cells with unrepaired DNA damage. The system of cyclins and cyclin-dependent kinases (CDK), which provide coordination of mitotic events during passage of a cell through the cell cycle, plays a crucial role in regulation of cell proliferation.Aim.To evaluate the relationship of single-nucleotide polymorphisms (SNPs) of cell cycle genes with an increased frequency of СD in workers of a nuclear power plant affected by chronic occupational radiation exposure in the dose range of 100–500 mSv.Materials and methods.The object of the study was blood of 55 conditionally healthy workers of Siberian Chemical Plant (SCP) who were affected by chronic occupational radiation exposure (gamma radiation) in the dose range of 100–500 mSv. A standard cytogenetic analysis of blood lymphocytes was performed for all examined individuals. Genomic DNA was isolated from the blood of the workers using the QIAamp DNA Blood Mini Kit (QIAGEN, Germany). DNA was genotyped using 257 SNPs of cyclin genes and neighboring intergenic regions using DNA microarrays from the high-density CytoScan HD Array (Affymetrix, USA).Results.Taking into account the Bonferroni correction, only statistically significant associations of SNPs with the frequency of dicentric chromosomes were found; all other types of chromosomal aberrations did not show statistical significance. The rs803054CCNI2was associated with an increased frequency of dicentric chromosomes arising under the influence of chronic occupational radiation exposure.Conclusion.The discovered SNP (rs803054), whose recessive genotype is associated with an increased frequency of dicentric chromosomes in workers of SCP exposed to radiation at doses of 100–500 mSv over a long time, can be considered as a potential marker of individual radiosensitivity. To confirm the identified associations, further validation studies are needed on an expanded sample of people affected by chronic occupational radiation exposure.
Heart failure with preserved ejection fraction: the role of microvascular dysfunction
Authors: Kopeva K. V. , Mochula A. V. , Maltseva A. N. , Grakova E. V. , Shipulin V. V. , Gusakova A. M. , Zavadovsky K. V. 
Published: January 2023

Aim. To evaluate the relationship between coronary microvascular dysfunction (CMD), biomarkers of cardiac fibrosis and cardiac remodeling (soluble ST2 (sST2), fibroblast growth factor-23 (FGF-23), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), and NT-proBNP), parameters of diastolic dysfunction (DD), and the presence of heart failure with preserved ejection fraction (HFpEF) in symptomatic patients.Materials and methods. Study participants were 59 patients with non-obstructive coronary artery disease (CAD) and preserved left ventricular ejection fraction (LVEF) of 62 (56; 67) %. Non-obstructive CAD was verified by coronary computed tomography angiography. Stress-and rest-myocardial blood flow (MBF) and coronary flow reserve (CFR) parameters were evaluated by CZT SPECT. Serum levels of cardiac biomarkers were measured by the enzyme immunoassay. Two-dimensional transthoracic echocardiography was used to assess DD parameters.Results. Decreased CFR was defined as CFR ≤ 2. Therefore, CMD was defined as the presence of decreased CFR in the absence of flow-limiting CAD. Distribution of patients was performed by CFR values: group 1 included patients with preserved CFR (>2, n = 35), and group 2 encompassed patients with decreased CFR (≤2, n = 24). In 87.5% of cases, patients with CMD were diagnosed with HFpEF, whereas in patients with preserved CFR, heart failure was diagnosed only in 51.4% of cases (p < 0.0001). CFR values were correlated with the left atrial volume (r = –0.527; p = 0.001), E / A ratio (r = –0.321, p = 0.012), and E / e’ (r = –0.307; p = 0.021). Following the ROC analysis, the levels of sST2 ≥ 31.304 ng / ml (AUС = 0.730; р = 0.004) and NT-proBNP ≥ 0.034 pg / ml (AUС = 0.815; р = 0.034) were identified as cut-off values for the presence of CMD in patients with non-obstructive CAD.Conclusion. The obtained data suggest that CMD may play an essential role in HFpEF. The values of CFR were correlated with DD parameters, and decreased CFR was associated with overexpression of biomarkers of cardiac fibrosis and cardiac remodeling. Serum levels of sST2 and NT-proBNP were identified as cut-off values for the presence of CMD in patients with non-obstructive CAD.
Effects of a high-fat, high-carbohydrate diet on the retina of young and old rats
Authors: Logvinov S. V. , Mustafina L. R. , Kurbatov B. K. , Naryzhnaya N. V. , Varakuta E. Yu. , Potapov A. V. 
Published: January 2023

Aim. To study the effect of a high-fat, high-carbohydrate diet on retinal morphology of young and old rats in the experiment.Materials and methods. The study was carried out on male Wistar rats aged 60 and 450 days at the beginning of the experiment. The animals were divided into 4 groups: group 1 (n = 14) included intact rats aged 150 days at the end of the experiment; group 2 (n = 14) encompassed rats (60 days old) fed with a high-fat, high-carbohydrate diet (HFHCD) for 90 days; group 3 (n = 14) included intact rats (450 days old) receiving a standard diet for 90 days; group 4 (n = 14) included rats (450 days old) fed with HFHCD for 90 days. Immunoassay and histology were used in the work.Results. HFHCD resulted in an increase in glucose concentration in animals of both age groups. In old animals, it caused a pronounced increase in the content of insulin, TGFβ, and fibronectin in the blood serum, neovascularization of outer retinal layers, as well as karyopyknosis and death of neurosensory cells, leading to destruction of photoreceptors and drastic thinning of the outer nuclear and outer plexiform layers. In young rats fed with HFHCD, no pronounced histologic disorders of the retina were noted.Conclusion. HFHCD enhances age-related retinal changes in old (450-day-old) rats.
La Educación Física en el nivel inicial desde un enfoque sociocultural
Authors: Dupuy Manuel , Gómez Smyth Leonardo 
Published: January 2023

La presente investigación tiene como objetivo caracterizar las prácticas pedagógicas progresivas en educación física en el nivel inicial, buscando describir, la voz de los docentes: la función social que se le asigna al área, el conocimiento de la cultura corporal, la concepción del juego y la modalidades de planificación que proponen. El diseño metodológico es descriptivo (Samaja, 1994), síncrono/transaccional (Hernández Sampieri, Fernández Collado y Batista Lucio, 2006), con un muestreo probabilístico, de tipo aleatorio simple (Ynoub, 2015) en el que el momento es de 50 docentes. que actualmente se encuentran trabajando en el sistema educativo (nivel inicial) han sido entrevistados. Las prácticas pedagógicas progresivas (entre innovación y transformación) en la Educación Física del Nivel Inicial sustentan que el área curricular promueva el derecho al juego y la construcción de la identidad corporal de los niños y niñas como ejes permanentes de experiencia en los encuentros, estableciendo mismo como conocimiento. asociado a la cultura corporal. Las modalidades de planificación son abiertas, flexibles, teniendo presente la palabra de lxs niños y articulando con la acción de evaluación formativa; Para ellos utilizan como formatos el cuaderno de planificación bitácora/caducado y el proyecto de educación física.
Infection from an Iliosacral Screw 16 Years Postoperatively in Demolition Derby Umpire Crushed Between 2 Cars
Authors: Brahme Indraneel S. , Hu Caroline H. , Cole Peter A. 
Published: January 2023

Case:
A 47-year-old man crushed between 2 cars during a demolition derby, a nonracing North American motorsport, underwent open reduction and internal fixation, iliosacral screw fixation, arterial embolization, and multiple urologic procedures after massive pelvic ring injury, remarkably recovering nearly full function. Sixteen years after injury, he developed an abscess emanating from an iliosacral screw requiring irrigation, debridement, and hardware removal.

Conclusion:
Deep surgical infections from iliosacral screws may present late, even more than 15 years after the original surgery. Obesity, preoperative embolization, diabetes, and urethral injuries are relevant risk factors. Similar patients should have a low threshold for infection workup when presenting with symptoms, even years after surgery.
Grateful Personality Development of Junior High School Students in East Kalimantan
Authors: Afandi Nur Kholik , Pranajaya Syatria Adymas 
Published: January 2023

A grateful personality is one of the essential elements in the personality development of students. This personality is reflected in verbal and deeds as a manifestation of acknowledgment in the heart for the blessings. This personality encourages students to appreciate and repay the kindness of others. This study aims to determine the level of gratitude of junior high school students in East Kalimantan. This study used a mixed-method approach. Respondents in this study were 714 people. The mean test results showed that the level of students' gratitude at Junior High Schools in East Kalimantan was very high, with an average score of 4.6. The teacher's strategies in instilling a grateful personality are with advice, exemplary, assignments, habituation, and activity programs at school. The methods are by providing materials (lectures, advice, discussions, and questions and answers related to gratitude), exemplary by providing examples of the values of gratitude, giving demonstration tasks, and practice in gratitude, giving the task of making a list of good things that have been felt and should be grateful for every day, habituation to good things (instilling the nature of qana'ah, giving appreciation and motivation, presenting a sense of empathy around oneself, inviting self-introspection, giving charity, helping others, spreading smiles, greetings, visiting friends or teachers who are sick or grieving), and school programs (dhuha and dzuhur prayers in congregation, reading the Qur'an, praying before starting lessons, and giving sadaqah every Friday at school). The results of this study may have an implication to become a policy regulation for schools to support the development of a grateful personality for students of Junior High School.
Desórdenes informativos y polarización afectiva: en un análisis de las emociones en publicaciones con desinformación en tres conflictos políticos brasileños en Twitter.
Authors: Santana Chaves Julia Marks , De Camargo Penteado Claudio Luis 
Published: January 2023

El artículo presenta un estudio sobre la desinformación y la polarización afectiva en las plataformas digitales. La investigación analizó la presencia y el tipo de desórdenes informativos y su relación con la movilización de emociones en 3 conflictos brasileños en Twitter: legalización del aborto, caso de racismo y apertura de investigación por irregularidades en acciones de combate a la Covid-19 en el país. Mediante técnicas computacionales de monitoreo, recolección y procesamiento de datos extraídos de la plataforma, se compuso una muestra de los 100 mensajes más compartidos de cada uno de los conflictos (n=300). Estas publicaciones fueron analizadas cualitativamente para identificar y clasificar el tipo de desórdenes informativos y verificar la movilización del marco de emociones en estos tweets con desinformación. Los resultados muestran que en los tres conflictos analizados fue posible identificar una gran cantidad de contenidos con desórdenes informativos entre los posts más compartidos y su estrecha relación con las emociones negativas (ira y asco), indicando la relación entre la desinformación y la polarización afectiva.
Neural and behavioral effects of parent training on emotion recognition in mothers rearing children with ADHD
Authors: Makita Kai , Yao Akiko , Shimada Koji , Kasaba Ryoko , Fujisawa Takashi X , Mizuno Yoshifumi , Tomoda Akemi 
Published: January 2023

Abstract
This randomized controlled study examined neurological changes in socioemotional processing skills through parent training in caregivers of children with attention-deficit/hyperactivity disorder. Thirty mothers of children with attention-deficit/hyperactivity disorder were stratified into parent training and non-parent training groups. Functional magnetic resonance imaging was performed during the “Reading the Mind in the Eyes” test, and parenting difficulties were evaluated using the Parenting Stress Index and the Parenting Scale, twice (before and after parent training). Only mothers in the parent training group showed a significant decrease in Parenting Stress Index and Parenting Scale scores. They also demonstrated increased activity in the left occipital fusiform gyrus during the task of estimating emotions from facial pictures, with a trend towards a reduced response time while judging others’ emotional state in the “Reading the Mind in the Eyes” test. We presumed that these changes might reflect that enrollment in parent training could reduce stress, which in turn might possibly reduce reaction times in processing others’ emotional states. Furthermore, these results may also be related to the increased activation of the fusiform gyrus.
Why Nations Prosper
Authors: Natadipurba Chandra 
Published: January 2023

Abstract
This paper's main idea is that Synechi causes prosperity. I define "Synechi" as "nation political leader implemented and reinforced a series of free-market policy continuously without substantial reverse for at least 20 years." I use: (1) the difference-in-difference method, using West Germany, South Korea, Botswana as the treatment group and East Germany, North Korea, and Zimbabwe, as the control group, to mimic "natural experiment", (2) Direct Method of Agreement from data of 52 countries and (3) Method of Difference form data of 24 countries and (4) Joint Method of Agreement and Difference, from data of 66 countries. I use Maddison Project Database data in the long- run GDP per capita published in early 2018 and other data sources. This approach is novel since previous papers only show that the free-market correlates with prosperity.
JEL Classification:
F59 O10 O11 O20 O21 POO
Effects of biochar combined with MgO desulfurization waste residue on nitrogen conversion and odour emission in chicken manure composting
Authors: Pang Yuwan , Zhen Feng , Wang Dehan , Luo Zifeng , Huang Jianfeng , Zhang Yanli 
Published: January 2023

Abstract
Aim
Chicken manure can readily produce much odour during aerobic composting. This odour pollutes the surrounding environment and represents the loss of nutrients such as nitrogen and sulfur, which reduces the fertilizer quality.
Methods
In this paper, biochar combined with MgO desulfurization waste residue (MDWR) was used as a new composting additive. Through composting tests, characterization analysis and pot experiments, the effect of additives in compost on nitrogen retention and deodorization was studied, the mechanism of in situ chemical deodorization was revealed, and the compost quality was verified.
Results
The results indicated that biochar and MDWR addition optimally reduced ammonia volatilization in chicken manure compost, and the reduction rate reached 60.12%. Volatile organic compound (VOC) emissions originating from chicken manure compost containing biochar and MDWR decreased by 44.63% compared to those originating from the control group.
Conclusions
The total nitrogen (TN) in the composting product containing chicken manure treated with both biochar and MDWR (CMB) was 67.7% higher than that in the composting product under the blankcontral, and this value was significantly higher than that under the other treatments and better reflected the synergistic effect of these two additives on nitrogen retention. Struvite crystals could be formed in the CMB treatment. According to the pot experiment results in terms of compost products, the CMB treatment increased the yield and quality of vegetables and reduced the nitrate content. Therefore, MDWR significantly affects nitrogen retention and deodorization of chicken manure compost while improving the compost quality; therefore, MDWR exhibits broad application prospects.
Comprehensive treatment strategy for banana inflorescence bract to synthesize biodiesel and bioethanol through fungal biorefinery
Authors: Bharathi Sundaram Deepika , Jacob Samuel 
Published: January 2023

Abstract
Banana inflorescence bract (BIB), an agro-waste is sporadically explored for second-generation biofuel production in spite of having considerable holocellulosic composition (cellulose-35.56%, w/w; hemicellulose-22.41%, w/w). In this study, an attempt has been made to utilize this substrate for fermentable sugar extraction (C5 and C6) which were employed for the co-production of microbial lipids and ethanol using Rhodosporidium toruloides NCIM 3547 and Saccharomyces cerevisiae respectively. Since, a considerable amount of lignin (8.78%, w/w) is present in BIB, a hybrid pretreatment and carbohydrate hydrolysis through microwave (160 W) assisted mild H2SO4 acid 2.5% (v/v) was adopted. The resultant liquor contains holocellulosic sugars (C5 and C6 sugars), out of which xylose (10.40 ± 0.49 g/L) and glucose (51. 48 ± 1.14 g/L). Hence, it was used as the growth medium for Rhodosporidium toruloides to produce lipids i.e., single cell oil (SCO). The maximum lipid content was found to be 41.59 ± 1.16 (%, w/w) containing total saturated fatty acids of 89.07% which justifies its potential application in biodiesel production. On the other hand, the pretreated solid fraction containing cellulose was saccharified using cellulolytic enzyme produced by Aspergillus sp. with saccharification of 69.99 ± 0.30 (%, v/w) and 27.22 g/L of reducing sugar. This enzymatic hydrolysate was used for ethanol production by Saccharomyces cerevisiae resulting in an ethanol yield of 12.70 ± 0.09 (g/L) and productivity of 0.132 g/L/h. BIB is a potential feedstock and thereby, paving a sustainable route for agro-waste management by favouring integrated production of biodiesel and bioethanol towards a biorefinery approach.
Exosomal miR-320e is a potential target of CVSD that regulates the Wnt2-mediated Wnt/β-catenin signaling pathway
Authors: Wang Zheng , Li Xuening , Yang Shaonan , Wang Yuan , Gao Kejin , Han Bin , Ma Aijun 
Published: January 2023

Abstract
Exosomal miRNAs play crucial roles in many central nervous system diseases. Cerebral small vessel disease (CVSD) is a small vessel disease thatis affected by various factors. In the present study, we investigated the role of exosomal miR-320e in theWnt/β-catenin pathway stimulated by oxidative stress and assessed its role in CVSD.
The differentially expressed exosomal miRNAs were filtered by sequencing plasma exosomes from CVSD patients and healthy controls. Bioinformatic and dual luciferase analyses were used to confirm the relationship between Wnt2 and miR-320e. Quantitative real-time PCR and Western blotting were used to detect the mRNA and protein levels of Wnt/β-catenin pathway components. Membrane fluorescence staining was used to detect exosome transfer.
High-throughput sequencing showed that exosomal miR-320e was downregulated. Bioinformatics analysis and dual-luciferase reporter gene experiments showed that exosomal miR-320e regulated Wnt2expression by targeting the 3' noncodingregion of Wnt2. Exosomal miR-320e was found to mediate the response of the Wnt/β-catenin signaling pathway to oxidative stress through loss-of-function experiments using mimics, inhibitors and knockdown/overexpression lentivirus. Exosomal miR-320e could target and inhibit the Wnt2/β-catenin signaling pathway.
Our research suggests that exosomal miR-320e is a suppressor of the Wnt/β-catenin signaling pathway and may play a protective role in the progression of CVSD.
Clinical trial registration
Not applicable
Bacteroides ovatus alleviates dysbiotic microbiota-induced intestinal graft-versus-host disease
Authors: Jenq Robert , Hayase Eiko , Hayase Tomo , Mukherjee Akash , Stinson Stuart , Jamal Mohamed , Ortega Miriam , Sanchez Christopher , Ahmed Saira , Karmouch Jennifer , Chang Chia-Chi , Flores Ivonne , McDaniel Lauren , Brown Alexandria , El-Himri Rawan , Chapa Valerie , Tan Lin , Tran Bao , Pham Dung , Halsey Taylor , Jin Yimei , Tsai Wen-Bin , Prasad Rishika , Glover Israel , Ajami Nadim , Wargo Jennifer , Shelburne Samuel , Okhuysen Pablo , Liu Chen , Fowler Stephanie , Conner Margaret , Peterson Christine , Rondon Gabriela , Molldrem Jeffrey J , Champlin Richard , Shpall Elizabeth , Lorenzi Philip , Mehta Rohtesh , Martens Eric , Alousi Amin 
Published: January 2023

Abstract
Acute gastrointestinal intestinal GVHD (aGI-GVHD) is a serious complication of allogeneic hematopoietic stem cell transplantation, and the intestinal microbiota is known to impact on its severity. However, an association between treatment response of aGI-GVHD and the intestinal microbiota has not been well-studied. In a cohort of patients with aGI-GVHD (n=37), we found that non-response to standard therapy with corticosteroids was associated with prior treatment with carbapenem antibiotics and loss of Bacteroides ovatus from the microbiome. In a mouse model of carbapenem-aggravated GVHD, introducing Bacteroides ovatus reduced severity of GVHD and improved survival. Bacteroides ovatus reduced degradation of colonic mucus by another intestinal commensal, Bacteroides thetaiotaomicron, via its ability to metabolize dietary polysaccharides into monosaccharides, which then inhibit mucus degradation by Bacteroides thetaiotaomicron and reduce GVHD-related mortality.
Does Renewable Energy Improve Environmental Quality? Evidence from RECAI Countries
Authors: Raghutla Chandrashekar , Kolati Yeliyya 
Published: January 2023

Abstract
Since 1990, the ecological footprints have been increasing significantly with a continuous increase rate, which led to challenges to environmental quality. The basis for Economic growth was said to be the shift of energy and environmental strategies toward a sustainable future. Indeed, it became a matter of proclaimed acceptance that environmental challenges nurtured expansion, innovation, and competitiveness. Climate change is the most pressing issue being faced by the world due to an increase in ecological footprint from 7.0 billion GHA to 20.6 billion GHA. It indicates the seriousness of environmental degradation; therefore, the nations need to ensure environmental sustainability. Keeping this in mind, the present research main aims to examine the impact of renewable energy utilization on the ecological footprints of RECAI economies, spanning the period 1990 to 2020. To significantly achieve the research objective, we utilized panel econometric methods for empirical analysis. The results of long-run elasticities indicate that both the renewable energy utilization as well as trade openness significantly controls the ecological footprints, while higher conventional energy utilization and economic growth significantly impede the environmental sustainability. The empirical findings provide new insights for policymakers on renewable energy for the betterment of environmental quality in RECAI countries.