bluuebunny/crossref_metadata_2025_split huggingface dataset:
Commotio Cordis in 2023
Authors: Peng Theodore , Derry Laura Trollinger , Yogeswaran Vidhushei , Goldschlager Nora F. 
Published: June 2023

AbstractSince the nationally televised cardiac arrest of American National Football League player Damar Hamlin in January 2023, commotio cordis has come to the forefront of public attention. Commotio cordis is defined as sudden cardiac arrest due to direct trauma to the precordium resulting in ventricular fibrillation or ventricular tachycardia. While the precise incidence of commotio cordis is not known due to a lack of standardized, mandated reporting, it is the third most common cause of sudden cardiac death in young athletes, with more than 75% of cases occurring during organized and recreational sporting events. Given that survival is closely tied to how quickly victims receive cardiopulmonary resuscitation and defibrillation, it is crucial to raise awareness of commotio cordis so that athletic trainers, coaches, team physicians, and emergency medical personnel can rapidly diagnose and treat this often-fatal condition. Broader distribution of automated external defibrillators in sporting facilities as well as increased presence of medical personnel during sporting events would also likely lead to higher survival rates.
Development and Validation of a New Tool to Measure Performance Knowledge and Communication Skill of Multidisciplinary Health Science Learners on Radiation Emergency Preparedness and Response Management
Authors: Bhushan Shivanand , Malapure Sumeet Suresh , D’Souza Nisha Rachel , Tandon Pankaj , Oommen Sibi , G. Srinidhi , Verma Ashwani Kumar , Pandey Akhilesh K. , Wilson William , Kulkarni Muralidhar M. 
Published: June 2023

Abstract

Objective:
The purpose of the study was to design, develop, and validate a newer tool on radiation emergency preparedness responses (RadEM-PREM IPE tool) to measure communication, knowledge, performance skills in multidisciplinary health science learners.

Methods:
The study design is a prospective, single centric, pilot study. Five subject experts designed, analyzed, and selected items of the instrument for relevant content and domain. Psychometrics that the tool assessed were content validity, internal consistency, test-retest reliability, and intraclass correlation coefficient. Twenty-eight participants completed test-retest reliability for validation of 21 sorted out items calculated percentage of agreement >70% I-CVI/UA (item content validity index with universal acceptability) and S-CVI/UA (scale content validity index with universal agreement method).

Results:
Items with percentage agreement >70% and I-CVI over 0.80 were kept, ranged from 0.70 to 0.78 were revised, and below 0.70 were rejected. Items with kappa values ranging from 0.04 to 0.59 were revised and ≥0.74 were retained. Internal consistency assessed using Cronbach’s alpha was 0.449. Positive correlation between attitude and communication (r = 0.448), between performance and communication (r = 0.443) were statistically significant at 0.01 level. Overall, intraclass correlation coefficient for all the measures is 0.646, which is statistically significant at 0.05 level (P < 0.05).

Conclusions:
Study concludes that the RadEM-PREM IPE tool would be a new measuring tool to assess knowledge, performance, and communication skills of interprofessional radiation emergency response team learner’s evaluation.
Readying Community Pharmacies to Participate in COVID-19 Testing and Vaccination
Authors: Bacci Jennifer L. , Shah Parth D. , Arnold Jenny , Atkins Dana L. , Weiner Bryan J. 
Published: June 2023

Abstract

Objective:
Washington State established a Memorandum of Understanding (MOU) and operational plan in 2012 to coordinate pharmacy infrastructure and workforce during a public health emergency. The objectives of this study were to adapt the MOU operational plan to the context of the coronavirus disease 2019 (COVID-19) pandemic and assess community pharmacies’ organizational readiness to implement COVID-19 testing and vaccination.

Methods:
This mixed methods study was conducted June-August 2020. Three facilitated discussions were conducted with community pharmacists and local health jurisdiction (LHJ) representatives to test the MOU operational plan. Facilitated discussions were thematically analyzed to inform adaptations to the operational plan. Pharmacists were surveyed to assess their organization’s readiness for COVID-19 testing and vaccination before and after the facilitated discussions using the Organizational Readiness for Implementing Change (ORIC) measure. Survey responses were analyzed using descriptive statistics.

Results:
Six pharmacists from 5 community pharmacy organizations and 4 representatives from 2 LHJs participated in at least 1 facilitated discussion. Facilitated discussions resulted in 3 themes and 16 adaptations to the operational plan. Five of 6 community pharmacists (83% response rate) completed both surveys. Mean organizational readiness decreased from baseline to follow-up for COVID-19 testing and vaccination.

Conclusions:
Operational plan adaptations highlight opportunities to strengthen MOUs between local and state health departments and community pharmacies to support future emergency preparedness and readiness efforts.
Regular consumption of lacto-fermented vegetables has greater effects on the gut metabolome compared with the microbiome
Authors: Guse Kylene , Sharma Ashok , Weyenberg Emily , Davison Sam , Ma Yiwei , Choi Yuni , Johnson Abigail J. , Chen Chi , Gomez Andres 
Published: June 2023

Abstract
The industrialisation of Western food systems has reduced the regular consumption of lacto-fermented vegetables (LFV). Consuming LFV may exert health benefits through the alteration of the gut microbiome, but the mechanisms involved remain unclear. To start understanding the possible benefits of LFV, we compared faecal microbial diversity and composition, as well as dietary habits between individuals who regularly consume LFV (n = 23) and those who do not (n = 24). We utilised microbial DNA amplicon sequencing (16S rRNA and ITS2) and untargeted metabolomics (LC–MS) to analyse stool samples. Study participants also provided three consecutive days of dietary data. Results show minor effects on microbiome composition; with the enrichment of a few microorganisms potentially associated with vegetable ferments, such as Leuconostoc mesenteroides and Rhodotorula mucilaginosa (P < 0.05), in LFV consumers. However, LFV consumption had greater effects on the faecal metabolome, with higher abundances of butyrate, acetate, and valerate (P < 0.05) and significantly greater metabolome diversity (P < 0.001). Overall, the observations of minor changes in the faecal microbiome and greater effects on the faecal metabolome from LFV consumption warrant further investigations on the health significance of LFV as regular components of the daily diet in humans.
Use of a severe acute respiratory coronavirus virus 2 (SARS-CoV-2) strand-specific assay to evaluate for prolonged viral replication >20 days from illness onset
Authors: Ferguson Jessica D. , Tayyar Ralph , Contreras Gustavo , Kiener Melanie , Zimmet Alex N. , Contag Caitlin A. , Rodriguez Nava Guillermo , Tompkins Lucy S. , Shepard John , Rosenthal Ayelet , Subramanian Aruna K. , Pinsky Benjamin A. , Salinas Jorge L. 
Published: June 2023

AbstractSevere acute respiratory coronavirus virus 2 (SARS-CoV-2) real-time reverse-transcription polymerase chain reaction (rRT-PCR) strand-specific assay can be used to identify active SARS-CoV-2 viral replication. We describe the characteristics of 337 hospitalized patients with at least 1 minus-strand SARS-CoV-2 assay performed >20 days after illness onset. This test is a novel tool to identify high-risk hospitalized patients with prolonged SARS-CoV-2 replication.
Causal associations between dietary habits and CVD: a Mendelian randomisation study
Authors: Yang Miaomiao , Gao Xiong , Xie Liangzhen , Lin Zhizhan , Ye Xingsheng , Ou Jianyan , Peng Jian 
Published: June 2023

AbstractOver the years, numerous observational studies have substantiated that various dietary choices have opposing effects on CVD. However, the causal effect has not yet been established. Thus, we conducted a Mendelian randomisation (MR) analysis to reveal the causal impact of dietary habits on CVD. Genetic variants strongly associated with 20 dietary habits were selected from publicly available genome-wide association studies conducted on the UK Biobank cohort (n 449 210). Summary-level data on CVD were obtained from different consortia (n 159 836–977 323). The inverse-variance weighted method (IVW) was the primary outcome, while MR-Egger, weighted median and MR Pleiotropy RESidual Sum and Outlier were used to assess heterogeneity and pleiotropy. We found compelling evidence of a protective causal effect of genetic predisposition towards cheese consumption on myocardial infarction (IVW OR = 0·67; 95 % CI = 0·544, 0·826; P = 1·784 × 10−4) and heart failure (IVW OR = 0·646; 95 % CI = 0·513, 0·814; P = 2·135 × 10−4). Poultry intake was found to be a detrimental factor for hypertension (IVW OR = 4·306; 95 % CI = 2·158, 8·589; P = 3·416 × 10−5), while dried fruit intake was protective against hypertension (IVW OR = 0·473; 95 % CI = 0·348, 0·642; P = 1·683 × 10−6). Importantly, no evidence of pleiotropy was detected. MR estimates provide robust evidence for a causal relationship between genetic predisposition to 20 dietary habits and CVD risk, suggesting that well-planned diets may help prevent and reduce the risk of CVD.
Tetrathyridia in an endemic lizard from Chile: molecular evidence for South America
Authors: Correa J. P. , Farías F. , San Juan E. , Yáñez-Meza A. , Muñoz-Leal S. , Botto-Mahan C. , Oyarzún-Ruiz P. 
Published: June 2023

Abstract
Mesocestoides is a controversial tapeworm with significant lack of data related to systematics and life cycles. This helminth has an indirect life cycle with vertebrates, mostly carnivorous mammals, as definitive hosts. Theoretically, a coprophagous arthropod would be the first intermediate host, and herptiles, mammals, and birds, which prey on these insects, would represent the second intermediate hosts. However, recent evidence suggests that this life cycle would require only two hosts, with no arthropods involved. In the Neotropics, although there are records of mammals and reptiles as hosts for Mescocestoides, no molecular analyses have been performed. This work aimed to record an additional intermediate host and molecularly characterize the isolated larvae. Thus, 18 braided tree iguanas (Liolaemus platei) from Northern Chile were collected and dissected during 2019. One lizard was parasitized by three morphotypes of larvae compatible with tetrathyridia of Mescocestoides. To achieve its specific identity, a molecular approach was performed: 18S rRNA and 12S rRNA loci were amplified through cPCR. The inferred phylogenies confirmed the morphological diagnosis and stated that all morphotypes were conspecifics. The sequences for both loci formed a monophyletic clade with high nodal support, representing a sister taxon to Mescocestoides clade C. This study represents the first molecular characterization of any taxon of Mescocestoides from the Neotropics. Future surveys from potential definitive hosts would help to elucidate its life cycle. Furthermore, an integrative taxonomic approach is required in additional studies from the Neotropical region, which would contribute to a better understanding of the evolutionary relationships of this genus.
Cardiovascular manifestations and cardiac magnetic resonance follow-up of multisystem inflammatory syndrome in children (MIS-C)
Authors: Karagözlü Selen , Ramoğlu Mehmet G. , Bayram Özlem , Bakhtiyarzada Jeyhun , Aydın Alperen , Yılmaz Mehmet Mustafa , Murt Begüm , Özkan Ersin , İnceli Hatice Belkıs , Gurbanov Anar , Şükriye Yılmaz , Demir Berrin , Özdemir Halil , Çiftçi Ergin , Kendirli Tanıl , Uçar Tayfun , Fitoz Ömer Suat , Tutar Ercan 
Published: June 2023

AbstractObjective:This study aimed to evaluate the cardiovascular manifestations and surveillance of multisystem inflammatory syndrome in children (MIS-C) and to determine the correlation of echocardiographic findings with cardiac magnetic resonance imaging findings.Methods:Forty-four children diagnosed as MIS-C with cardiac involvement were enrolled in this observational descriptive study. The diagnosis of MIS-C was made according to the criteria of Centers for Disease Control and Prevention. Clinical findings, laboratory parameters, and electrocardiographic and echocardiographic findings at the time of diagnosis and during follow-up were evaluated. Cardiac magnetic resonance was performed on 28 (64%) cases. The 1-year follow-up imaging was performed in all cases with abnormal initial cardiac magnetic resonance findings.Results:Forty-four patients (56.8% male) with a mean age of 8.5 ± 4.8 years were enrolled in this study. There was a significant positive correlation between high-sensitivity cardiac troponin T (mean: 162 ± 444.4 pg/ml) and N-terminal pro b-type natriuretic peptide (mean: 10,054 ± 11,604 pg/ml) (p < 0.01). Number of cases with an electrocardiographic and echocardiographic abnormality was 34 (77%) and 31 (70%), respectively. Twelve cases (45%) had left ventricular systolic dysfunction and 14 (32%) cases had pericardial effusion on admission. Three cases (11%) had cardiac magnetic resonance findings that may be attributed to the presence of myocardial inflammation, and pericardial effusion was present in seven (25%) cases. Follow-up cardiac magnetic resonances of all cases were normal. Cardiac abnormalities were completely resolved in all except two cases.Conclusions:Myocardial involvement can be seen during acute disease, but MIS-C generally does not lead to prominent damage during a year of surveillance. Cardiac magnetic resonance is a valuable tool to evaluate the degree of myocardial involvement in cases with MIS-C.
Dosimetric comparison of three-dimensional (3D), intensity-modulated radiotherapy (IMRT) and hybrid IMRT for left-sided postmastectomy radiation therapy (PMRT)
Authors: Thongthanom Siwika , Nobnop Wannapha 
Published: June 2023

Abstract

Purpose:
This study aimed to determine the suitable breast treatment technique for a small facility’s hospital with limited staff and equipment resources. The benefits and drawbacks of each technique should be considered to guide radiation oncologists choose the appropriate treatment option for postmastectomy radiation therapy (PMRT) patients.

Methods and Materials:
This study included the computed tomography images of 15 patients who received left-sided PMRT. The patient’s characteristics were classified into two groups: 1. irradiation of only the chest wall (CW) and 2. CW lymph nodes plus supraclavicular lymph nodes (SPCs). All 15 PMRT patients were generated in 4 treatment techniques including 3DCRT, field-in-field (FiF), intensity-modulated radiotherapy (IMRT) and hybrid (3DCRT + IMRT). Each treatment technique’s dosimetric parameters and treatment time were compared.

Result:
All four treatment plans met the acceptable criteria. The IMRT plans achieved the highest plan quality scores for two groups of PMRT patients but require the longest treatment time, whereas the 3DCRT and FiF plans demonstrated superiority for organ at risk (OAR) sparing and required the shortest treatment time when compared with the IMRT and hybrid plans.

Conclusion:
The IMRT plan had the highest plan quality but required the most time to treat. Treatment times are critical in facilities with limited resources. As a result, the FiF plan was found to be a suitable technique for both CW-only and CW plus SPC irradiation due to its short treatment time and high plan quality scores for OAR dose sparing.
How Do Earthquakes Stop? Insights From a Minimal Model of Frictional Rupture
Authors: Barras Fabian , Thøgersen Kjetil , Aharonov Einat , Renard François 
Published: June 2023

AbstractThe question “what arrests an earthquake rupture?” sits at the heart of any potential prediction of earthquake magnitude. Here, we use a one‐dimensional, thin‐elastic‐strip, minimal model, to illuminate the basic physical parameters that may control the arrest of large ruptures. The generic formulation of the model allows for wrapping various earthquake arrest scenarios into the variations of two dimensionless variables (initial dimensionless stress parameter on the fault) and (dimensionless fracture energy), valid for both in‐plane and antiplane shear loading. Our continuum model is equivalent to the standard Burridge‐Knopoff model, with an added characteristic length scale, H, that corresponds to either the thickness of the damage zone for strike‐slip faults or to the thickness of the downward moving plate for subduction settings. We simulate the propagation and arrest of frictional ruptures and derive closed‐form expressions to predict rupture arrest under different conditions. Our generic model illuminates the different energy budget that mediates crack‐ and pulse‐like rupture propagation and arrest. It provides additional predictions such as generic stable pulse‐like rupture solutions, stress drop independence of the rupture size, the existence of back‐propagating fronts, and predicts that asymmetric slip profiles arise under certain pre‐stress conditions. These diverse features occur also in natural earthquakes, and the fact that they can all be predicted by a single minimal framework is encouraging and pave the way for future developments of this model.
Non‐Fossil Origin Explains the Large Seasonal Variation of Highly Processed Organic Aerosol in the Northeastern Tibetan Plateau (3,200 m a.s.l.)
Authors: Ni Haiyan , Yao Peng , Zhu Chongshu , Qu Yao , Tian Jie , Ma Yongyong , Yang Lu , Zhong Haobin , Huang Ru‐Jin , Dusek Ulrike 
Published: June 2023

AbstractCarbonaceous aerosol plays an important role in climate, but its sources and atmospheric processes are least understood in the Tibetan Plateau (TP), a remote yet climatically sensitive region. This study presents the first seasonal cycle of radiocarbon and stable isotope 13C of organic and elemental carbon (OC and EC) in the atmosphere of the northeastern TP. Large seasonal variations of EC and OC concentrations were explained by non‐fossil sources. Regardless of the season, fossil contribution to OC was strongly correlated with inverse OC concentrations. This allowed the separating a constant background source and a source responsible for OC variability that was mostly of non‐fossil origin. The 13C signature of OC shows that OC was highly atmospherically processed and thus less volatile than OC found near sources or in urban areas. The 13C‐depleted secondary sources contributed strongly to more volatile OC, whereas the 13C‐enriched less volatile OC suggests the influence of atmospheric aging.
MultiVI: deep generative model for the integration of multimodal data
Authors: Ashuach Tal , Gabitto Mariano I. , Koodli Rohan V. , Saldi Giuseppe-Antonio , Jordan Michael I. , Yosef Nir 
Published: June 2023

AbstractJointly profiling the transcriptome, chromatin accessibility and other molecular properties of single cells offers a powerful way to study cellular diversity. Here we present MultiVI, a probabilistic model to analyze such multiomic data and leverage it to enhance single-modality datasets. MultiVI creates a joint representation that allows an analysis of all modalities included in the multiomic input data, even for cells for which one or more modalities are missing. It is available at scvi-tools.org.
Versatile properties of dynein molecules underlying regulation in flagellar oscillation
Authors: Fujiwara Takashi , Shingyoji Chikako , Higuchi Hideo 
Published: June 2023

AbstractDynein is a minus-end-directed motor that generates oscillatory motion in eukaryotic flagella. Cyclic beating, which is the most significant feature of a flagellum, occurs by sliding spatiotemporal regulation by dynein along microtubules. To elucidate oscillation generated by dynein in flagellar beating, we examined its mechanochemical properties under three different axonemal dissection stages. By starting from the intact 9 + 2 structure, we reduced the number of interacting doublets and determined three parameters, namely, the duty ratio, dwell time and step size, of the generated oscillatory forces at each stage. Intact dynein molecules in the axoneme, doublet bundle and single doublet were used to measure the force with optical tweezers. The mean forces per dynein determined under three axonemal conditions were smaller than the previously reported stall forces of axonemal dynein; this phenomenon suggests that the duty ratio is lower than previously thought. This possibility was further confirmed by an in vitro motility assay with purified dynein. The dwell time and step size estimated from the measured force were similar. The similarity in these parameters suggests that the essential properties of dynein oscillation are inherent to the molecule and independent of the axonemal architecture, composing the functional basis of flagellar beating.
Time-dependent cytokines changes in ultra-rush wasp venom immunotherapy
Authors: Urbańska W. , Szymański L. , Ciepelak M. , Cios A. , Stankiewicz W. , Klimaszewska E. , Lieto Krystyna , Skopek Rafał , Chciałowski A. , Lewicki S. 
Published: June 2023

AbstractVenom immunotherapy (VIT) represents a potential therapeutic approach for the management of venom allergies, aiming to modify the immune response to venom allergens and enhance its precision. Previous studies have demonstrated that VIT induces a shift in T helper cell responses from Th2 to Th1, characterized by the production of IL-2 and interferon-gamma by CD4+ and CD8+ cells. In order to explore long-term pathways following VIT treatment and verify potential new outcomes, the serum concentrations of 30 cytokines were assessed in a cohort of 61 patients (18 control, 43 study group) exhibiting hypersensitivity to wasp venom. Cytokine levels were measured at 0, 2, 6, and 24 weeks after the initiation phase of VIT in the study group. The present study found no significant alterations in the levels of IL-2 and IFN-γ in the peripheral blood following VIT. However, a noteworthy finding was the substantial increase in the concentration of IL-12, a cytokine capable of promoting the differentiation of Th0 cells into Th1 cells. This observation supports the involvement of the Th1 pathway in the desensitization process induced by VIT. Additionally, the study revealed a significant rise in the levels of IL-9 and TGF-β after VIT. These cytokines may play a role in the generation of inducible regulatory T (Treg) cells, indicating their potential importance in the immune response to venom allergens and the desensitization process associated with VIT. Nevertheless, further investigations are required to comprehend the underlying mechanisms driving the VIT process comprehensively.