bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Listeria monocytogenes in meat products during 2017–2019 depending on technological factors and seasons
Authors: Yushina Yu. K. , Kuznetsova O. A. , Tutelyan A. V. , Grudistova M. A. , Bataeva D. S. , Reshchikov M. D. , Tartakovsky I. S. , Nikolaev Yu. A. 
Published: December 2022

Microbiological examination of contamination of imported and domestic meat products with pathogenic bacteria Listeria monocytogenes depending on a meat type, technology and season was carried out during 2017–2019. In total, 2777 product samples were analyzed; the presence of this pathogen was revealed in 8.8% of products (244 positive samples). It was found that the prevalence of L. monocytogenes in meat products increased over three years of observation (2017–2019). The highest occurrence of this pathogen was found in poultry meat (on average 18.7%) followed by products from beef (13.2%). Meat products from mixed raw materials (beef and pork) accounted for 5.3% of tested samples, while in pork semi-finished products L. monocytogenes was found only in 3.2% of cases. It was noted that the technology of semi-finished products significantly affected the level of contamination of meat products with L. monocytogenes. Various technological approaches are used in the production process increasing the risk of contamination of the finished product since there is no timely data on Listeria contamination of raw materials used for production of a particular product. It has been established that a significant role in microbiological studies is played by various approaches to sample preparation of analyzed samples of meat cuts, semi-finished products in large and small pieces, as well as minced semi-finished products. Not knowing the real level of surface contamination with L. monocytogenes of carcasses, half-carcasses, semi-finished products in large pieces, manufacturers use such raw materials for the subsequent production of other types of semi-finished meat products, increasing the risk of manufacturing unsafe products with following contamination of equipment, work surfaces and other objects of the production environment. The highest occurrence of L. monocytogenes in meat products during three years of observation was found in the summer period (14.2%). The proportions of positive samples in the winter, spring and autumn months varied on average within 6.7–7.1%.
Study of adipose tissue of Kemerovo piglets: Detection of beige adipocytes
Authors: Pchelkina V. A. , Chernukha I. M. , Korotkiy I. A. , Ilyin N. A. 
Published: December 2022

Animals have two types of adipose tissue differing in structure and function: white (WAT) and brown (BAT). Beige adipose tissue (BeAT) is a result of WAT browning, when beige adipocytes appear between white adipocytes in response to exposure to cold, diet or physical activity. BeAT shares morphological and biochemical characteristics with BAT, is thermogenic and dissipates energy in the form of heat, unlike WAT, which is responsible for energy storage. Pigs do not have classic BAT, and modern breeds are sensitive to cold. There is literature information that BeAT has been found in cold-resistant pigs. The aim of the work was to study adipose tissue of piglets of the Kemerovo cold-resistant breed under growing conditions in the cold season and to identify the localization of BeAT. Histological studies have shown two types of adipocytes in subcutaneous fat samples (lateral, backfat and axillary): white, with one large fat droplet, and beige, multilocular. Larger cells were detected in backfat fat (69.36±12.98 µm) compared with lateral (53.25±9.27 µm) and axillary fat (45.94±8.29 µm). Only WAT with a diameter of 35.69±6.96 µm was present in the internal perirenal fat. Raman spectroscopy was used to evaluate the overall fatty acid profile of the tested samples. The main peaks were noted in all samples: 970 cm-1 (=C-H out-of-plane bend), 1266/1272 cm-1 (=C-H symmetrical rock) and 1655 cm-1 (C=C stretching) responsible for unsaturated bonds, and signals at 1297/1301 cm-1 (CH2 twisting), 1430/1460 cm-1 (CH2 symmetrical deformation (scissoring)) and 1735/1746 cm-1 (C=O stretching) corresponding to saturated bonds or ester groups. Internal perirenal fat contained the largest number of saturated fatty acid bonds, subcutaneous axillary fat — the smallest. The average intensity of the peaks was 0.4801010 and 0.639995, respectively. According to the results of gas chromatography, the largest amount of polyunsaturated fatty acids was noted in the subcutaneous fat samples: 20.199 in backfat fat, 21.749 in lateral and 20.436 in axillary fat compared to 18.636 in internal fat. Activation of beige cells in Kemerovo pigs under cold exposure, according to the authors, plays a crucial role in the heat balance, allowing them to tolerate cold without severe shivering. The study of the BeAT formation is of great practical importance for changing energy metabolism and increasing thermogenesis in newborn piglets by genome editing, as well as for improving the quality of pig’s fat.
The ways to improve the biological and morphological parameters of young fattening pigs
Authors: Mikolaychik I. N. , Morozova L. A. , Iltyakov A. V. , Stupina E. S. , Neverova O. P. , Uryumtseva T. I. 
Published: December 2022

Studies were carried out to find the new ways to improve the efficiency of iodine use for the young pigs. During the scientific and economic experiment it was found that bringing iodine to the physiological norm, in combination with introduction of 3% bentonite clay into the diets of young fattening pigs, provided a positive effect on the morphological composition of carcasses, slaughter parameters and meat quality of the pigs. So fattening the yelts with the diets with a physiological norm of iodine in combination with 3% bentonite clay allowed young pigs of the 3rdexperimental group to reach a slaughter weight of 78.95 kg at the age of 8 months, which is 10.1% more than in the control group, and 4.2% and 2.5% more in comparison with the 1st and 2nd experimental groups. The slaughter yield in yelts of the 3rdexperimental group increased by 2.43% (P<0.05) compared to the control group. The largest (30.17 cm2) area of the “rib eye” was recorded in the 3rdexperimental group, which is 5.60% more than the control one, while the carcasses of pigs of the 3rdexperimental group contained muscle tissue by 4.74% (P<0.05) more than the same in the control group. Analysis of the Musculus longissimus showed a significant increase in protein content in the 3rdexperimental group by 1.22% compared to the control group (P<0.05). The protein-quality parameter was significantly higher in the muscle tissue of young pigs of the 3rdexperimental group and amounted to 10.90 (P<0.05), which is 1.12% higher than in the muscle tissue of the control group yelts. The maximum content of oleic acid was found in the lard of animals of the 3rdexperimental group — 49.59, which is 1.28% (P<0.05) higher than in the control group.
Development of a new generation flotation cell and monitoring of air bubbles
Authors: Aytac Ayca , Tuna Muhammed Cihat 
Published: December 2022

Abstract
The flotation process allows particles and oil to separate from wastewater with high efficiency. Therefore, it is widely used in engineering and is a multidisciplinary field of study. In this study, a new generation flotation method is developed as an alternative to conventional flotation methods. Some experiments are conducted to determine the performance of this new system. It is known that the air-demand rate in the flotation process directly improves flotation performance. For this purpose, maximum aeration efficiency and bubble properties in the flotation cell supported by the newly developed head gated conduit are examined. A pilot-scale flotation system is installed for the experimental study. With the help of high resolution and high-speed cameras, parameters such as air bubble density, air bubble size, dead zone volume and penetration depth are determined. In addition, the images recorded during the flotation process are examined with professional image processing techniques. Experimental results showed that the Froude number, jet plunge angle and cell water levels have a significant impact on air-demand in the new system.
Pluralizacja religijna a proces integracji europejskiej. Wybrane zagadnienia
Authors: Popiuk-Rysińska Irena 
Published: December 2022

Celem artykułu jest identyfikacja i zbadanie wybranych zależności między zróżnicowaniem religijnym a procesem integracji europejskiej. Integrująca się Europa, podzielona początkowo generalnie na katolików i protestantów, uległa pluralizacji pod względem religijnym, przede wszystkim ze względu na napływ ludności prawosławnej i muzułmańskiej, wskutek dołączania nowych państw do pierwotnej integrującej się grupy oraz procesów migracyjnych. Każda z tych religii ma w jakimś zakresie wypracowaną własną wizję Europy, tożsamości oraz integracji, które oddziałują na postawy społeczne i poglądy polityków. Można zauważyć między innymi zróżnicowanie postaw odnośnie do integracji między katolikami, protestantami, prawosławnymi i muzułmanami, jak również wewnątrz tych wyznań, zależnie od wskazań wiary oraz stopnia zróżnicowania religijności ich wyznawców. Oprócz pośredniego oddziaływania, religia wywiera wpływ poprzez działanie podmiotów „religijnych”, w tym Kościołów, chadeckich partii politycznych, organizacji czy polityków. W artykule, przy odwołaniu do wybranych badań, analizowany jest wpływ pluralizmu religijnego na genezę i rozszerzanie się integracji, jej pogłębianie oraz ideowy wymiar, a także na instytucjonalizację kwestii religijnych w procesie integracji.
HIF-1α/Malat1/miR-141 Axis Activates Autophagy to Increase Proliferation,
Migration, and Invasion in Triple-negative Breast Cancer
Authors: Zhang Chi , Xu Fangyuan , Hu Yue , Gao Jie , Wang Jianxiong , Xie Yujie , Sun Fuhua , Wang Li , Miyamoto Akira , Xia Ou 
Published: December 2022

Background:
The mechanism of metastasis-associated lung adenocarcinoma transcript 1 (Malat1) in triple-negative breast cancer (TNBC) is still unclear.

Objective:
This study aimed to investigate the role of miR-141-3p and Malat1 in autophagy in TNBC under hypoxia.

Method:
The expression levels of Malat1 and miR-141-3p were detected via quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression levels of hypoxia-inducible factor 1α (HIF-1α), HIF-2α, MMP9, p62 and LC3 were determined via western blotting. A Cell Counting Kit-8 assay was used to detect cell viability, while a Transwell assay to detect cell proliferation and invasion. A luciferase assay was used to confirm the relationship between Malat1 and miR-141-3p.

Results:
A significant increase was observed in the expression level of Malat1 and the autophagic activity in TNBC tissues and cells. The expression level of Malat1 was higher in a hypoxic environment, which can significantly promote the proliferation, migration, and invasion of TNBC cells by activating autophagy. HIF-1α, but not HIF-2α, was identified to induce the upregulation of Malat1 in TNBC cells. The dual-luciferase assay results identified a miR-141-binding site in Malat1. Malat1 knockdown and miR-141-3p overexpression were demonstrated to significantly inhibit autophagy, thereby inhibiting cell proliferation, invasion, and migration. Moreover, hypoxia can inhibit the effect of miR-141-3p on TNBC cells.

Conclusion:
miR-141-3p could suppress autophagy and inhibit proliferation, migration, and invasion by targeting Malat1 in TNBC cells under hypoxia. The existence of the HIF-1α/Malat1/miR-141 axis plays a vital role in the development of TNBC and may be a target for the diagnosis and treatment of TNBC.
Effects of Exercise on Vascular Toxicity Associated with Breast Cancer
Treatment: A Narrative Review
Authors: Pera Marina L.D. , Speretta Guilherme F. 
Published: December 2022

Abstract:
Breast cancer is the most common cancer among women worldwide, and its incidence is
linearly associated with age. The development of cancer treatments has changed the prognosis of this
disease. Despite effective treatments, cardiovascular complications in middle-aged and older women
have become challenging. Physical exercise is a powerful tool to prevent senescence symptoms and
diseases, besides being an essential component for cardiovascular diseases and cancer prevention and
treatment. The present narrative review considers the vascular dysfunction associated with breast cancer
treatment, specifically chemotherapy and radiotherapy, and the effects of exercise on vascular toxicity.
We also explored the mechanisms involved in these responses. The search strategy involved three databases
(Pubmed, Scielo, and Web of Science) with the following descriptors: breast cancer, vascular
toxicity, physical exercise, chemotherapy, and radiotherapy. The evidence showed that breast cancer
patients, especially those under chemotherapy and over 50 years old, have a potential risk of developing
vascular dysfunction, which may persist in the long term. Decreases in nitric oxide bioavailability and
increases in oxidative stress and pro-inflammatory cytokines might mediate the chemotherapy and radiotherapy-
induced vascular dysfunction. Exercise seems to be a promising strategy for managing this
risk. However, there is a need for well-constructed studies evaluating vascular toxicity in breast cancer,
especially in middle-aged and elderly patients, to establish whether exercise is beneficial.
Cellulose Nanocrystals Prepared by TEMPO-mediated Oxidation of Wild
Musa spp. Pseudostem
Authors: Nath Ranjita , Pachuau Lalduhsanga 
Published: December 2022

Abstract:
Valorization of agricultural waste to produce value-added products such as nanocellulose
is important in bringing sustainable development and reduce our dependence on petroleumbased
products which are harmful to our environment. The present work is carried out to investigate
the potential of the pseudostems from the wild Musa spp. as a novel sustainable source of
cellulose nanocrystal (CNCs). TEMPO-mediated oxidation and ultrasonication was followed in
isolation of CNCs. The CNCs were characterized by various physicochemical parameters such as
FTIR, TEM, Zeta potential, degree of oxidation, swelling and water retention value. TGA studies
was performed to evaluate the thermal stability and percent crystallinity was determined by XRD
spectroscopy. The final carboxylated CNC (cCNC) gel contains 5.56% w/w solid CNC exhibiting
degree of oxidation of 34.91%. The CNC also showed high water retention value exhibiting
ionic sensitivity to NaCl. The zeta potential value was determined to be -50.3 mV indicating its
stability and particle size of the cCNC was less than 200 nm. The percent crystallinity was found
to be 66.18% and TGA analysis showed the reduced thermal stability of the cCNCs. Carboxylated
CNC was successfully isolated from the wild banana pseudostem following TEMPOoxidation
method. Ultrasonication of the cCNC resulted in the formation of cCNCs with mostly
spherical in shapes and the results from analysis indicate that the wild banana pseudostem could
be a potential sustainable source of cellulose nanocrystals.
Improving the Chemical and Sensory Properties of Cheddar Cheese Using
Adjunct Cultures
Authors: Khosravi-Darani Kianoush , Massoud Ramona 
Published: December 2022

Background:
Dairy consumption and especially cheese consumption has been increasing
in most countries of the world due to its favorable taste. European countries and the
United States are the major producers and consumers of cheese. Due to the nutritional and economic
importance of cheese, various schemes have been considered to increase its consumption
and the most important of which is to improve its sensory properties. Several factors affect the
development of cheese properties including chemical composition, residual coagulation factors,
enzymes in milk, microbial flora of cheese and the storage conditions during the ripening stage,
microorganisms in cheese especially lactic acid bacteria.

Objective:
Various methods have been studied to improve the sensory properties of cheese in the
world and the most successful of which is the addition of adjunct culture. Adjunct cultures are a
variety of selected microorganisms that are added to milk to improve the sensory quality or to
accelerate the ripening of the product. The major adjunct cultures are lactobacilli. Many studies
show that Lactobacillus helveticus LH212 and Lactobacillus helveticus CNRZ32 are among the
most desirable bacteria. Adjunct cultures are used in two ways: attenuated and live cultures.
There are several methods for preparing attenuated cultures, but most researchers focus on physical
methods such as heat shock, freezing shock and spray drying.

Conclusion:
The purpose of this article is to express the role of adjunct cultures (types of Lactobacilli)
in the sensory properties of cheddar cheese, selection criteria, and the amount, and time
of adding adjunct cultures in the cheese-making process.
Deep Eutectic Solvents: Fundamental Aspect, Characterizations and Applications
Authors: Dangre Pankaj V. , Borase Harshada P. , Gunde Mahendra C. , Pethe Anil M. , Borkar Maheshkumar R. 
Published: December 2022

Abstract:
Deep eutectic solvents (DESs) containing bioactive have been explored as potential choices
for therapeutic efficiency enhancement. DESs are regarded as superior compared to established solvents
owing to accessibility, storage conditions, synthesis, and low cost. As such, intensive research
has taken place in different disciplines, especially nutraceuticals, foods and pharmaceuticals. The applications
of DESs, especially in nutraceuticals and pharmaceutical delivery, have shown great promise.
Despite these different successes, the safety issues of these DESs need to be properly identified. A
safe mixture of DESs must be developed to take its broad range of advantages to the nutraceutical industry,
and, therefore, its nutraceutical applications can only be introduced if DESs are known to have
profiles of negligible or minimal toxicity. This review emphasizes the fundamental aspects needed to
have a better understanding of DESs. It covers the current prospects of DES, including types, properties,
formulation components and characterization methods. The several characterization methods,
viz., pH, density, refractive index, viscosity, surface tension, solubility, polarized optical microscopy,
x-ray diffraction studies, Fourier transforms infrared spectroscopy, and nuclear magnetic resonance
spectroscopy are also mentioned. Further, the promising applications of DESs in different nutraceutical
and pharmaceutical domains are highlighted.
The Potential for Using Extended Reality Technology in Interdisciplinary Case Discussions and Case Planning in Stereotactic Radiosurgery: Proof-of-Concept Usability Study
Authors: Chidambaram Swathi , Palumbo Maria Chiara , Stifano Vito , McKenna John , Redaelli Alberto , Olivi Alessandro , Apuzzo Michael , Pannullo Susan 
Published: December 2022

Background
Extended reality (XR) is a term that captures a variety of techniques, such as augmented reality (AR) and mixed reality (MR), which allow users to interact with virtual models in real time. This technology has an emerging role in several applications within neurosurgery. XR can be useful in enhancing how radiosurgical cases are planned. Multidisciplinary team (MDT) review is an essential part of the radiosurgery case planning process; during case discussions, patient images are reviewed, usually in 2D or 3D modifications. The current commercially available platforms for this review need improvement.

Objective
We describe a novel visualization application, titled “NeuroVis” by our development team, which uses an XR Microsoft HoloLens headset to provide an interactive 3D visualization of a patient’s neuroanatomy in stereotactic surgery (SRS) case planning discussions.

Methods
We present examples of 6 common radiosurgery indications to demonstrate the utility of NeuroVis to solve common visualization hurdles in MDTs.

Results
The utility of NeuroVis is demonstrated through 6 common brain tumor SRS cases as a proof-of-concept illustration of the utility of NeuroVis to enhance radiosurgery case discussion by improving visualization of the standard neuroimaging used in radiosurgery treatment planning by MDTs.

Conclusions
The NeuroVis application provides several interactive features that produce an enhanced ability to place participating members of an interdisciplinary treatment team on the same visualization plane. This technology, by facilitating team discussions and case review, has the potential to improve the efficiency, efficacy, and safety of radiosurgery treatment planning and, as a result, to optimize patient care.
Swasthya Pahal (Health for All) Using a Sustainable, Multisector, Accessible, Affordable, Reimbursable, and Tailored Informatics Framework in Rural and Urban Areas of Chennai, Tamil Nadu: Protocol for a Quantitative Study
Authors: Joshi Ashish , Surapaneni Krishna Mohan , Grover Ashoo , Kaur Harpreet , Saggu Sofia Rani , Oliveira Doilyn 
Published: December 2022

Background
Noncommunicable diseases (NCDs) require a longer period of care, for which health care systems must acquire technologically advanced solutions to enhance patient care. Swasthya Pahal (health for all) is an innovative, interactive, multilingual, stand-alone, internet-enabled computer-based program that aims to improve the self-management of NCDs.

Objective
This study aims to enhance the self-management of chronic NCDs (diabetes, hypertension, high cholesterol, and obesity) by determining the usefulness, acceptance, and effectiveness of the Swasthya Pahal program in hospital and community settings in both rural and urban areas of Chennai, Tamil Nadu. This objective can be met by generating risk factor profiles of individuals enrolled and enhancing their self-management of NCDs using a portable health information kiosk that uses the Sustainable, Multisector, Accessible, Affordable, Reimbursable, and Tailored (SMAART) model.

Methods
A quantitative study will be conducted on a convenient sample of 2800 individuals from selected hospital and community settings in rural (n=1400) and urban areas (n=1400) in Chennai, Tamil Nadu. Data will be collected on sociodemographics, health behaviors, and clinical status, as well as knowledge, attitudes, and practices. Objective assessments such as weight, blood pressure, and random blood sugar levels will be measured. In addition, the usefulness, acceptance, and effectiveness of the Swasthya Pahal program will be determined.

Results
Results will be summarized using descriptive analysis. Appropriate bivariate and multivariate regression analysis will be performed to determine the predictors of the outcome variables of usefulness, acceptance, and effectiveness of Swasthya Pahal in wider settings. All analyses will be performed using SAS (version 9.1; SAS Institute), and the results will be reported as 95% CI values and P<.05.

Conclusions
The study proposes to enhance the self-management of NCDs in both rural and urban community settings through the implementation of the Swasthya Pahal program based on the SMAART informatics framework. The study aims to understand the implementation, acceptability, and usability of Swasthya Pahal among a diverse sample of people in urban and rural settings.

International Registered Report Identifier (IRRID)
PRR1-10.2196/39950