bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-voluntarism, natural providence and miracles in Thomas Burnet's Theory of the Earth
Authors: Rossetter Thomas 
Published: December 2022

AbstractIn his Telluris Theoria Sacra and its English translation The Theory of the Earth (1681–90), the English clergyman and schoolmaster Thomas Burnet (c.1635–1715) constructed a geological history from the Creation to the Final Consummation, positing predominantly natural causes to explain biblical events and their effects on the Earth and life on it. Burnet's insistence on appealing primarily to natural rather than miraculous causes has been interpreted both by his contemporaries and by some historians as an essentially Cartesian principle. On this reading, Burnet adhered to a Cartesian style of explanation in which there was no place for miracles. In this paper, I propose a different interpretation. Burnet's commitment to natural over miraculous causes, I argue, was grounded in an anti-voluntarist theology which he inherited from the Cambridge Platonists and Latitudinarians. This anti-voluntarism, moreover, also dictated the kind of miracles to which he did appeal. This reading of Burnet contrasts with the view that he was simply following Cartesian principles. First, Descartes had espoused a radical form of theological voluntarism. Second, Burnet's and Descartes's views of providence were based on distinct attributes of God, and these attributes had quite different implications regarding the place of miracles in the providential order.
Women's Suffrage, Political Economy, and the Transatlantic Birth Strike Movement, 1911–1920
Authors: Shew Tania 
Published: December 2022

AbstractThe first two decades of the twentieth century saw the development of a meaningful transnational movement to employ birth strikes in the fight for women's rights. In an Anglo-American context, this movement was intimately tied to the women's suffrage campaign. It was led by a network of suffragists, Neo-Malthusians, and birth control campaigners who shared literary and personal ties which allowed their ideas to criss-cross the Atlantic between 1911 and 1920. Although the transatlantic birth strike was never implemented on a significant scale, explaining its almost total absence within existing historiography, this article uses a gendered intellectual history framework to piece together the ideas behind the movement which, the article argues, disrupt established understanding of Neo-Malthusianism and socialist-feminism within intellectual histories. Support for birth striking was predicated on faith in the power of working-class collective action, scrutiny of the economic exploitation of both productive and ‘reproductive’ workers, and a corresponding mistrust in the efficacy of state involvement with these issues. The birth strikers wove together strands from collectivist, individualist, socialist, and feminist thought, undermining traditional historiographical depictions of binaries between socialism and suffragism and collectivism and individualism in early twentieth-century political thought.
‘Farming with alternative pollinators’ approach supports diverse and abundant pollinator community in melon fields in a semi-arid landscape
Authors: Bencharki Youssef , Christmann Stefanie , Lhomme Patrick , Ihsane Oumayma , Sentil Ahlam , El Abdouni Insafe , Hamroud Laila , Rasmont Pierre , Michez Denis 
Published: December 2022

AbstractThe presence of pollinating insects in crop fields is an essential factor for agricultural production and pollinator conservation. Agricultural intensification has been identified as a driver of pollinator decline over the last decades and challenges the efficiency of pollination. Several approaches are used to support pollinators and their ecosystem services, notably reward-based wildflower strips. ‘Farming with Alternative Pollinators’ (FAP) aims to attract and sustain pollinators using marketable habitat enhancement plants (MHEP) in the field borders instead of wildflowers. These MHEP are selected in conjunction with farmers. We tested here whether the FAP approach increases diversity and abundance of flower visitors in melon fields in a semi-arid landscape in Morocco. Moreover, we examined whether MHEP increase flower-visitor abundance in melon flowers. We recorded a total of 1330 insect specimens including 573 specimens of wild bees.Lasioglossum malachurumwas the major flower visitor in melon and several MHEP. As flower-visitor abundance and diversity in FAP fields were higher than in control fields, we conclude that FAP can be a valuable approach for pollinator protection in agro-ecosystems; 16.5% of wild bees and wasps showed spillover from the field borders to the melon fields.
Utilization of image-based spectral reflectance to detect herbicide resistance in glufosinate-resistant and glufosinate-susceptible plants: a proof of concept
Authors: Jones Eric A. L. , Austin Robert , Dunne Jeffrey C. , Cahoon Charles W. , Jennings Katherine M. , Leon Ramon G. , Everman Wesley J. 
Published: December 2022

AbstractGlufosinate is an effective postemergence herbicide, and overreliance on this herbicide for weed control is likely to increase and select for glufosinate-resistant weeds. Common assays to confirm herbicide resistance are dose–response and molecular sequencing techniques; both can require significant time, labor, unique technical equipment, and a specialized skillset to perform. As an alternative, we propose an image-based approach that uses a relatively inexpensive multispectral sensor designed for unmanned aerial vehicles to measure and quantify surface reflectance from glufosinate-treated leaf disks. Leaf disks were excised from a glufosinate-resistant and glufosinate-susceptible corn (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean [Glycine max (L.) Merr.] varieties and placed into a 24-well plate containing eight different concentrations (0 to 10 mM) of glufosinate for 48 h. Multispectral images were collected after the 48-h incubation period across five discrete wave bands: blue (475 to 507 nm), green (560 to 587 nm), red (668to 682 nm), red edge (717 to 729 nm), and near infrared (842 to 899 nm). The green leaf index (GLI; a metric to measure chlorophyll content) was utilized to determine relationships between measured reflectance from the tested wave bands from the treated leaf disks and the glufosinate concentration. Clear differences of spectral reflectance were observed between the corn, cotton, and soybean leaf disks of the glufosinate-resistant and glufosinate-susceptible varieties at the 10 mM concentration for select wave bands and GLI. Leaf disks from two additional glufosinate-resistant and glufosinate-susceptible varieties of each crop were subjected to a similar assay with two concentrations: 0 and 10 mM. No differences of spectral reflectance were observed from the corn and soybean varieties in all wave bands and the GLI. The leaf disks of the glufosinate-resistant and glufosinate-susceptible cotton varieties were spectrally distinct in the green, blue, and red-edge wave bands. The results provide a basis for rapidly detecting glufosinate-resistant plants via spectral reflectance. Future research will need to determine the glufosinate concentrations, useful wave bands, and susceptible/resistant thresholds for weeds that evolve resistance.
Paliperidone palmitate vs. paliperidone extended-release for the acute treatment of adults with schizophrenia: a systematic review and pairwise and network meta-analysis
Authors: Kishi Taro , Sakuma Kenji , Iwata Nakao 
Published: December 2022

AbstractIs paliperidone palmitate (PP) a useful treatment option for adults with acute symptoms of schizophrenia? We conducted a systematic review and a random-effects pairwise and network meta-analysis that compared PP (25−150 mg equivalent) with paliperidone extended-release (PAL-ER, 3−12 mg/d) regarding their efficacy and safety in adults with acute symptoms of schizophrenia. The outcomes were the total score of the Positive and Negative Syndrome Scale (PANSS-T) at week 6 (the primary outcome for efficacy) and all-cause discontinuation(the primary outcome for acceptability), discontinuation due to inefficacy, discontinuation due to adverse events, discontinuation due to the withdrawal of consent, and the incidence of individual adverse events. Five studies on PP and seven studies on PAL-ER, which involved 4970 individuals in total, were included in this study. For the primary outcomes, we only included data from the treatment arms that used 100 or 150 mg equivalent as an initial dose of PP and data from the treatment arms that used 6, 9, or 12 mg as an initial dose of PAL-ER. The pairwise meta-analyses showed that both PP and PAL-ER outperformed placebo regarding PANSS-T at week 6 and all-cause discontinuation. However, there were no statistically significant differences in these outcomes between the effect sizes of PP and that of PAL-ER. Both PP and PAL-ER increased blood prolactin levels in both females and males compared with placebo. PAL-ER significantly increased blood prolactin in both females and males compared with PP. There were no statistically significant differences in other outcomes between the effect sizes of PP and that of PAL-ER. Similar results in all outcomes were observed in the network meta-analyses. In conclusion, PP might be a useful treatment option for adults with acute symptoms of schizophrenia. A noninferiority study that directly compares PP with PAL-ER for acute schizophrenia, conducted according to the recommended regimen, is required to provide solid evidence.
Glyco-binding domain chimeric antigen receptors as a new option for cancer immunotherapy
Authors: Franke Anna-Katharina , Wessolowski Charlotte , Thaden Vanessa , Müller Ingo , Cornils Kerstin 
Published: December 2022

AbstractIn the last decade, treatment using Chimeric Antigen Receptor (CAR) are largely studied and demonstrate the potential of immunotherapeutic strategies, as seen mainly for blood related cancers. Still, efficient CAR-T cell approaches especially for the treatment of solid tumors are needed. Tn- and Sialyl-Tn antigens are tumor associated carbohydrate antigens correlating with poor prognosis and tumor metastasis on a variety of tumor entities. These glycans can be recognized by CD301 (CLEC10A, MGL), which is a surface receptor found primarily on immune cells. In the present study, we hypothesized, that it is possible to use newly generated CD301-bearing CARs, enabling cytotoxic effector cells to recognize and eliminate breast cancer cells. Thus, we genetically modified human NK92 cells with different chimeric receptors based on the carbohydrate recognition domain (CRD) of human CD301. We assessed their cytotoxic activity in vitro demonstrating the specific recognition of CD301 ligand positive cell lines. These results were confirmed by degranulation assays and in cytokine release assays. Overall, this study demonstrates CD301-CARs represent a cost-effective and fast alternative to conventional scFv CARs for cancer immunotherapy.
Optical pulse labeling studies reveal exogenous seeding slows α-synuclein clearance
Authors: Croft Cara L. , Paterno Giavanna , Vause Ava R. , Rowe Lyla A. , Ryu Daniel H. , Goodwin Marshall S. , Moran Corey A. , Cruz Pedro E. , Giasson Benoit I. , Golde Todd E. 
Published: December 2022

AbstractThe accumulation of α-synuclein (α-syn) in intracellular formations known as Lewy bodies (LBs) is associated with several neurodegenerative diseases including Parkinson’s disease and Lewy Body Dementia. There is still limited understanding of how α-syn and LB formation is associated with cellular dysfunction and degeneration in these diseases. To examine the clearance and production dynamics of α-syn we transduced organotypic murine brain slice cultures (BSCs) with recombinant adeno-associated viruses (rAAVs) to express Dendra2-tagged human wild-type (WT) and mutant A53T α-syn, with and without the addition of exogenous α-syn fibrillar seeds and tracked them over several weeks in culture using optical pulse labeling. We found that neurons expressing WT or mutant A53T human α-syn show similar rates of α-syn turnover even when insoluble, phosphorylated Ser129 α-syn has accumulated. Taken together, this data reveals α-syn aggregation and overexpression, pSer129 α-syn, nor the A53T mutation affect α-syn dynamics in this system. Prion-type seeding with exogenous α-syn fibrils significantly slows α-syn turnover, in the absence of toxicity but is associated with the accumulation of anti-p62 immunoreactivity and Thiazin Red positivity. Prion-type induction of α-syn aggregation points towards a potential protein clearance deficit in the presence of fibrillar seeds and the ease of this system to explore precise mechanisms underlying these processes. This system facilitates the exploration of α-syn protein dynamics over long-term culture periods. This platform can further be exploited to provide mechanistic insight on what drives this slowing of α-syn turnover and how therapeutics, other genes or different α-syn mutations may affect α-syn protein dynamics.
Multivalency ensures persistence of a +TIP body at specialized microtubule ends
Authors: Meier Sandro M. , Farcas Ana-Maria , Kumar Anil , Ijavi Mahdiye , Bill Robert T. , Stelling Jörg , Dufresne Eric R. , Steinmetz Michel O. , Barral Yves 
Published: December 2022

AbstractMicrotubule plus-end tracking proteins (+TIPs) control microtubule specialization and are as such essential for cell division and morphogenesis. Here we investigated interactions and functions of the budding yeast Kar9 network consisting of the core +TIP proteins Kar9 (functional homologue of APC, MACF and SLAIN), Bim1 (orthologous to EB1) and Bik1 (orthologous to CLIP-170). A multivalent web of redundant interactions links the three +TIPs together to form a ‘+TIP body’ at the end of chosen microtubules. This body behaves as a liquid condensate that allows it to persist on both growing and shrinking microtubule ends, and to function as a mechanical coupling device between microtubules and actin cables. Our study identifies nanometre-scale condensates as effective cellular structures and underlines the power of dissecting the web of low-affinity interactions driving liquid–liquid phase separation in order to establish how condensation processes support cell function.
Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends
Authors: Maan Renu , Reese Louis , Volkov Vladimir A. , King Matthew R. , van der Sluis Eli O. , Andrea Nemo , Evers Wiel H. , Jakobi Arjen J. , Dogterom Marileen 
Published: December 2022

AbstractGrowing microtubule ends organize end-tracking proteins into comets of mixed composition. Here using a reconstituted fission yeast system consisting of end-binding protein Mal3, kinesin Tea2 and cargo Tip1, we found that these proteins can be driven into liquid-phase droplets both in solution and at microtubule ends under crowding conditions. In the absence of crowding agents, cryo-electron tomography revealed that motor-dependent comets consist of disordered networks where multivalent interactions may facilitate non-stoichiometric accumulation of cargo Tip1. We found that two disordered protein regions in Mal3 are required for the formation of droplets and motor-dependent accumulation of Tip1, while autonomous Mal3 comet formation requires only one of them. Using theoretical modelling, we explore possible mechanisms by which motor activity and multivalent interactions may lead to the observed enrichment of Tip1 at microtubule ends. We conclude that microtubule ends may act as platforms where multivalent interactions condense microtubule-associated proteins into large multi-protein complexes.
Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolution
Authors: Cao Yunlong , Jian Fanchong , Wang Jing , Yu Yuanling , Song Weiliang , Yisimayi Ayijiang , Wang Jing , An Ran , Chen Xiaosu , Zhang Na , Wang Yao , Wang Peng , Zhao Lijuan , Sun Haiyan , Yu Lingling , Yang Sijie , Niu Xiao , Xiao Tianhe , Gu Qingqing , Shao Fei , Hao Xiaohua , Xu Yanli , Jin Ronghua , Shen Zhongyang , Wang Youchun , Xie Xiaoliang Sunney 
Published: December 2022

AbstractContinuous evolution of Omicron has led to a rapid and simultaneous emergence of numerous variants that display growth advantages over BA.5 (ref. 1). Despite their divergent evolutionary courses, mutations on their receptor-binding domain (RBD) converge on several hotspots. The driving force and destination of such sudden convergent evolution and its effect on humoral immunity remain unclear. Here we demonstrate that these convergent mutations can cause evasion of neutralizing antibody drugs and convalescent plasma, including those from BA.5 breakthrough infection, while maintaining sufficient ACE2-binding capability. BQ.1.1.10 (BQ.1.1 + Y144del), BA.4.6.3, XBB and CH.1.1 are the most antibody-evasive strains tested. To delineate the origin of the convergent evolution, we determined the escape mutation profiles and neutralization activity of monoclonal antibodies isolated from individuals who had BA.2 and BA.5 breakthrough infections2,3. Owing to humoral immune imprinting, BA.2 and especially BA.5 breakthrough infection reduced the diversity of the neutralizing antibody binding sites and increased proportions of non-neutralizing antibody clones, which, in turn, focused humoral immune pressure and promoted convergent evolution in the RBD. Moreover, we show that the convergent RBD mutations could be accurately inferred by deep mutational scanning profiles4,5, and the evolution trends of BA.2.75 and BA.5 subvariants could be well foreseen through constructed convergent pseudovirus mutants. These results suggest that current herd immunity and BA.5 vaccine boosters may not efficiently prevent the infection of Omicron convergent variants.
Quantifying multiple stain distributions in bioimaging by hyperspectral X-ray tomography
Authors: Warr Ryan , Handschuh Stephan , Glösmann Martin , Cernik Robert J. , Withers Philip J. 
Published: December 2022

AbstractChemical staining of biological specimens is commonly utilised to boost contrast in soft tissue structures, but unambiguous identification of staining location and distribution is difficult without confirmation of the elemental signature, especially for chemicals of similar density contrast. Hyperspectral X-ray computed tomography (XCT) enables the non-destructive identification, segmentation and mapping of elemental composition within a sample. With the availability of hundreds of narrow, high resolution (~ 1 keV) energy channels, the technique allows the simultaneous detection of multiple contrast agents across different tissue structures. Here we describe a hyperspectral imaging routine for distinguishing multiple chemical agents, regardless of contrast similarity. Using a set of elemental calibration phantoms, we perform a first instance of direct stain concentration measurement using spectral absorption edge markers. Applied to a set of double- and triple-stained biological specimens, the study analyses the extent of stain overlap and uptake regions for commonly used contrast markers. An improved understanding of stain concentration as a function of position, and the interaction between multiple stains, would help inform future studies on multi-staining procedures, as well as enable future exploration of heavy metal uptake across medical, agricultural and ecological fields.
Photosynthesis of Au/TiO2 nanoparticles for photocatalytic gold recovery from industrial gold-cyanide plating wastewater
Authors: Kunthakudee Naphaphan , Ramakul Prakorn , Serivalsatit Karn , Hunsom Mali 
Published: December 2022

AbstractA series of Aux/TiO2 nanoparticles (NPs) with different gold loadings (x = 0.1–1.0 wt%) was synthesized by the photodeposition and then employed as photocatalysts to recover precious component from the industrial gold-cyanide plating wastewater. Effects of Au loading, catalyst dosage and types of hole scavenger on the photocatalytic gold recovery were investigated under ultraviolet–visible (UV–Vis) light irradiation at room temperature. It was found that different Au loadings tuned the light absorption capacity of the synthesized photocatalysts and enhanced the photocatalytic activity in comparison with the bare TiO2 NPs. The addition of CH3OH, C2H5OH, C3H8O, and Na2S2O3 as a hole scavenger significantly promoted the photocatalytic activity of the gold recovery, while the H2O2 did not. Among different hole scavengers employed in this work, the CH3OH exhibited the highest capability to promote the photocatalytic gold recovery. In summary, the Au0.5/TiO2 NPs exhibited the best photocatalytic activity to completely recover gold ions within 30 min at the catalyst dosage of 0.5 g/L, light intensity of 3.20 mW/cm2 in the presence of 20 vol% CH3OH as hole scavenger. The photocatalytic activity slightly decreased after the 5th cycle of recovery process, indicating its high reusability.
ACPred-BMF: bidirectional LSTM with multiple feature representations for explainable anticancer peptide prediction
Authors: Han Bingqing , Zhao Nan , Zeng Chengshi , Mu Zengchao , Gong Xinqi 
Published: December 2022

AbstractCancer has become a major factor threatening human life and health. Under the circumstance that traditional treatment methods such as chemotherapy and radiotherapy are not highly specific and often cause severe side effects and toxicity, new treatment methods are urgently needed. Anticancer peptide drugs have low toxicity, stronger efficacy and specificity, and have emerged as a new type of cancer treatment drugs. However, experimental identification of anticancer peptides is time-consuming and expensive, and difficult to perform in a high-throughput manner. Computational identification of anticancer peptides can make up for the shortcomings of experimental identification. In this study, a deep learning-based predictor named ACPred-BMF is proposed for the prediction of anticancer peptides. This method uses the quantitative and qualitative properties of amino acids, binary profile feature to numerical representation for the peptide sequences. The Bidirectional LSTM network architecture is used in the model, and the attention mechanism is also considered. To alleviate the black-box problem of deep learning model prediction, we visualized the automatically extracted features and used the Shapley additive explanations algorithm to determine the importance of features to further understand the anticancer peptide mechanism. The results show that our method is one of the state-of-the-art anticancer peptide predictors. A web server as the implementation of ACPred-BMF that can be accessed via:http://mialab.ruc.edu.cn/ACPredBMFServer/.