bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of pollen types of beekeeping interest by non‐targeted mass spectrometry
Authors: Leoni Valeria , Kast Christina , Bütikofer Ueli , Giupponi Luca , Portmann Reto 
Published: January 2025

AbstractBackgroundThe identification of pollen is important in the field of beekeeping for the determination of the botanical origin of bee products and investigations of bee diet. Until now, it has been performed by melissopalynology, the microscopic examination of pollen grains. However, this technique has some limitations, such as the necessity of experienced analysts and identification restricted to the family level for some pollen types. Although many techniques have been proposed as alternatives or complements to melissopalynology and omics techniques have been explored to gather information on the botanical origin of honey, no study has yet been conducted on a large set of pollen types.ResultsThe study dataset consisted of 34 different pollen types of pellets collected by honeybees in Switzerland and analyzed in multiple biological replications, leading to 150 observations. The pollen samples were analyzed after tryptic digestion using a non‐targeted mass spectrometry‐based method. Liquid chromatography coupled with mass spectrometry (LC–MS) was employed to identify pollen, and melissopalynology was used as a reference method for the identification. We built an OPLS‐DA prediction model for the 34 pollen types. The model clearly identified new samples in their membership group (Acer sp., n = 10) and a new pollen type at the species‐specific level for Quercus sp. Less predictable results were achieved for Composita H and pollen collected directly from the plant.ConclusionThe use of a non‐targeted mass spectrometry‐based method and chemometrics resulted in a promising tool for pollen identification as a replacement/supplement method to traditional melissopalynology.
Adsorption Performance and Mechanistic Pathways of Raw Powdered Pine Cone Toward the Removal of Dye and Cr(VI)
Authors: Wahiba Mecibah , Souad Bouasla , Mouna Cherifi , Djellabi Ridha , Nabila Boutemine , Djilani Chahrazed , Ferial Krid , Fayçal Djazi 
Published: January 2025

ABSTRACTThis work aims to valorize pine cones powder (PCP), an agricultural waste, as a bioadsorbent for the removal of a cationic dye, Rhodamine B (RhB), and hexavalent chromium Cr(VI) from an aqueous solution. The adsorbent was characterized by scanning electron microscopy (SEM), Fourier‐transformed infrared (FTIR), and X‐ray diffractometer (XRD) spectroscopy. Adsorption studies were carried out in a batch mode under different operating parameters like initial solution pH (2–11), adsorbent dosage (0.025–0.6 g), initial contaminant concentration (20–100 mg/L), temperature (283–328K), contact time (0–120 min), and ionic strength (NaCl, MgCl2, CuSO4, Na2SO4, and FeCl3). The optimum conditions for adsorption for Cr(VI) were; ([Cr(VI)] = 30 mg/L, pH = 2, adsorbent dose = 1.5 g/L, T = 25°C), and for RhB: ([RhB] = 30 mg/L, pH = 4.6, adsorbent dose = 4 g/L, T = 25°C). Under these conditions, the maximum adsorption capacities reached were 19.861 and 6.565 mg/g, for Cr(VI) and RhB, respectively. The inhibiting effect of the studied salts on RhB dye and metal ion removal is as follows: FeCl3 > CuSO4> MgCl2> Na2SO4> NaCl. The Freundlich isotherm described the best the adsorption of Cr (VI) and RhB onto PCP, since it gave the highest R2 values (R2 ≥ 0.983) associated with the lowest error values (χ2, RMSE, and Δq). The calculated Dubinin–Radushkevich mean energy (E) is less than 8 kJ/mol. It is 3.391 kJ/mol for Cr(VI) and 2.310 kJ/mol for RhB sorption, respectively, confirming the physical character of adsorption onto PCP sorbent. Experimental data for Cr(VI) and RhB ion adsorption onto PCP, fitted well the pseudo‐second‐order kinetic model (R2 ≥ 0.997). According to the thermodynamic analysis, the retention of Cr(VI) and RhB followed a physisorption, endothermic, and spontaneous process at all temperatures for Cr(VI), and at higher temperatures for RhB dye. These results suggest that raw PCP may be envisaged as a promising biosorbent for water remediation without the need of costly heat and activation processes as a cheap and sustainable adsorption process.
Soil Degradation of Sloping Cropland in the Typical Black Soil Region in Northeast China: Risk Assessment and Uncertainty Analysis
Authors: Zhang Shouhao , Sun Lei , Jamshidi Abdul Hakim , Niu Yong , Fan Zhaofei , Zhang Hongda , Liu Xia , Gao Lei 
Published: January 2025

ABSTRACTSoil degradation in the typical black soil region in Northeast China poses a threat to agricultural productivity and sustainability. However, little is known about the distribution of soil with different degradation degrees on a regional scale. This study aimed to map soil degradation status based on a newly developed soil degradation index (SDI) and assessed the uncertainty of degradation risk through the combination of ordinary Kriging and sequential Gaussian simulation (SGS) in order to enhance the accuracy and credibility of predictive results. The mean SDI was 0.445, with a coefficient of variation of 30%. The realization with 200 times by SGS was the best showed an increasing pattern of soil degradation degree from north to south in the study area. Slightly and moderately degraded sloping cropland accounted for 35.3% and 52.4% of all sloping cropland, respectively. High‐risk areas were mainly located in the Daxing'an and Changbai Mountains to the Songnen Plain. Proactive measures are necessary to control and mitigate further degradation by improving soil management and weakening the impact of runoff on sloping cropland. The developed risk assessment maps provide baseline information for regional sustainable development.
Spatiotemporal Response Relationships Between Changes in Water Conservation Service and Landscape Pattern in Zoige Plateau, China
Authors: Tang Xinhua , Zhuo Majie , Zhou Mecuo , Guo Shengmei , Gong Chunshan , Wang Jun , Yang Hao 
Published: January 2025

ABSTRACTUnderstanding the link between landscape pattern and water conservation service (WCS) is crucial for effective water resource conservation and landscape planning. However, the impact of landscape configuration on WCS remains unclear. This study explored the spatiotemporal relationships between WCS and landscape pattern in the Zoige Plateau (ZP), a vital waterhead of the yellow river, from 1990 to 2020. The results showed that woodland and high‐coverage grassland exhibited the highest WCS capacity and contributed the greatest to total WCS, suggesting the importance of protecting coverage and area of woodland and grassland. From 1990 to 2020, the areas of high‐ and medium‐coverage grasslands significantly decreased, implying adverse effects of landscape composition changes on ZP's WCS during the past few decades. The effects of landscape configuration metrics on WCS exhibited significant variability across years, climate scenarios and regions, highlighting the significant role of climatic and underlying surface. Under realistic climatic scenarios, for greater WCS, in most parts of the western and northern ZP, which negatively correlated with aggregation index, patch density, and mean fractal dimension index but positively correlated with shannon's diversity index, it is better to increase the proportion of large patches, reduce landscape fragmentation, and maintain diverse patch types. Conversely, in southeastern part of the central ZP, where WCS showed an opposite correlation with the aforementioned metrics, maintaining simpler patch type diversity and higher landscape connectivity may be useful. These findings provided important insights for identifying sensitive areas where landscape pattern changes affect WCS and optimizing landscapes to sustain ZP's WCS.
Recyclable Millable Polyurethane based on Enaminone Bonds With Upcycled Mechanical Performance
Authors: Li Xinyu , Ning Nanying , Yu Bing , Tian Ming 
Published: January 2025

AbstractThermoplastic polyurethane (TPU) exhibits re‐processable properties, but the properties of TPU is deteriorated during the reprocessing for the oxidation and degradation of polymer chains. Meanwhile, although thermoset polyurethane exhibits excellent mechanical properties, it cannot be recycled for permanent crosslinking. Hence, it's still a challenge to obtain PU which exhibits the balance between the recyclability and mechanical properties. In this work, a new dynamic bond obtained from the reaction between enaminone and isocyanate is used to prepare re‐processable millable polyurethane, and the morphology of network can be tuned via the dissociation of the cross‐linked sites of PU. Interestingly, the cross‐linked network can transform into a linear polymer by adding the amine which can be used when reacted with isocyanate to generate new re‐crosslinked PU. This process can be carried out in a Haake mixer without any solvents. The mechanical properties of the re‐crosslinked polyurethane can be tuned via the controlling of the amine and isocyanate addition, and the maximum tensile strength increase by 178.4% after processing for four times, realizing mechanical reinforcement after recycling. This kind of recycling achieves through one‐step melting method in solvent‐free conditions provides a feasible way to prepare recyclable PU with good mechanical performance and customizable properties.
TRIM36 Inhibits the Development of AOM/DSS‐Induced Colitis‐Associated Colorectal Cancer by Promoting the Ubiquitination and Degradation of GRB7
Authors: Wu Ju , Yang Zhengbo , Chen Xi , Hou Shuangshuang , Li Nanbo , Chang Yaoyuan , Yin Jiajun , Xu Jian 
Published: January 2025

ABSTRACTColorectal cancer (CRC) is among the most common cancer types for both sexes. Tripartite motif 36 (TRIM36) has been reported to be aberrantly expressed in several cancer types, suggesting its involvement in cancer progression. However, the role of TRIM36 in the colorectal carcinogenesis remain unknown. In our in vivo experiments, we investigated the role of TRIM36 in AOM/DSS‐induced colitis‐associated carcinogenesis using TRIM36‐knockout (TRIM36 KO) mice. Subsequently, we overexpressed and knocked down TRIM36 expression in two CRC cell lines to further confirm the role of TRIM36 in vitro. The UALCAN database revealed a significant decrease in TRIM36 levels in CRC tissues, including colon adenocarcinoma and rectum adenocarcinoma. A significant correlation was observed between TRIM36 levels and the histological subtype, individual cancer stage, and nodal metastasis status. The downregulation of TRIM36 in CRC tissues was further confirmed using our own collected clinical specimens. Low expression of TRIM36 was found to be associated with unfavorable overall survival and recurrence‐free survival in CRC. TRIM36 KO promoted inflammation, inhibited autophagy, and facilitated the development of AOM/DSS‐induced CRC. TRIM36 overexpression inhibited proliferation, migration, and invasion, while activated autophagy in CRC cells. TRIM36 directly bound to and regulated the ubiquitination of GRB7 protein. The tumor‐suppressive role of TRIM36 in CRC cells was mediated by GRB7. The TRIM36/GRB7 axis may represent a promising therapeutic target for the treatment of CRC.
Phenylbutyric Acid Modulates Apoptosis and ER Stress‐Related Gene Expression in Glycogen Storage Disease Type Ib In Vitro Model
Authors: Parezanovic Marina , Stevanovic Nina , Andjelkovic Marina , Ugrin Milena , Pavlovic Sonja , Stojiljkovic Maja , Skakic Anita 
Published: January 2025

ABSTRACTIntroductionChronic endoplasmic reticulum (ER) stress and increased apoptosis are involved in the pathogenesis of glycogen storage disease Ib (GSD Ib), whereas small molecule phenylbutyrate (4‐PBA) showed the capability of reducing ER stress‐induced apoptosis. The objective was to generate an in vitro system in which capability of small molecules (SMs) to influence ER stress and apoptosis could be screened at the expression level.MethodsG6PT‐deficient FlpInHEK293 cell line was created and validated using the CRISPR/Cas9 knockout method. Molecular markers of unfolded protein response (ATF4, DDIT3, HSPA5, XBP1s), and apoptosis (BCL2/BAX, CASP3, CASP7) in G6PT‐deficient cells were analyzed using RT‐qPCR method before and upon the treatment with 4‐PBA.ResultsTreatment with the most effective dose of 1 mM 4‐PBA reduced the expression of UPR markers and executioner caspases, while increased BCL2/BAX ratio in G6PT‐deficient cells. Our results proved the concept that 4‐PBA could alleviate markers of ER stress detected in the GSD Ib in vitro model system and prevent cell death.ConclusionThis cost‐effective in vitro model screens the therapeutic potential of SMs affecting ER stress and apoptosis in G6PT‐deficient kidney cells, offering a first‐line screening assay for promising compounds. 4‐PBA's potential repurposing for GSD Ib patients opens new research directions.
Enhanced Analysis of General Noncontinuous Stochastic Epidemic Model With Vaccination: A Novel Framework and Practical Application to COVID‐19 in Saudi Arabia
Authors: Sabbar Yassine , Adel Waleed , Elsonbaty Amr 
Published: January 2025

ABSTRACTThis paper introduces an enhanced analysis of a stochastic epidemic model that integrates vaccination and discontinuities. Our methodology initiates with formulating a perturbed model incorporating dual general incidence functions and a thorough distribution for jump components. After confirming the well‐posed nature of this model, we proceed to establish a global threshold criterion that discerns between the persistence and eradication of the infection. The accuracy of this threshold is scrutinized through numerical simulations utilizing real data related to COVID‐19 in Saudi Arabia. A distinctive feature of our approach is its departure from previous studies, as we provide a comprehensive framework and pinpoint optimal conditions for the model.
Do We Have Sufficient Evidence to Derive Innovative Approaches to Assessing Unmet Need, Delivering Education on Bladder and Bowel Continence Health, and Providing a Better Environment for Joint Decision‐Making? ICI‐RS 2024
Authors: Cotterill Nikki , Samarinas Michael , Rantell Angie , Selai Caroline , Arlandis Salvador , Jones Kathryn , Abrams Paul , Robinson D. , Wagg Adrian 
Published: January 2025

ABSTRACTContextImproved continence outcomes are reliant on identification of unmet need, education delivery, and shared decision‐making. The evidence base on which to derive innovative approaches in these areas was unclear.MethodsA debate held at the International Consultation on Incontinence‐Research Society meeting, held in Bristol in June 2024, considered ways to improve research requirements to advance these areas.Results and ConclusionArtificial intelligence solutions and digital approaches to healthcare are emerging at pace and offer possibilities to improve these three key areas but this must be driven by person‐centered approaches. Care must be taken to avoid increasing inequality through digital exclusion and language barriers. Research questions are highlighted to derive innovation in these three key areas.
Interpenetrating Polymer Network of Hydroxyethyl Methacrylate and Chitosan as Cryogels for Tissue Engineering Applications
Authors: Mishra Tiwari Ruchi , Channey Hareet Singh , Jain Era 
Published: January 2025

ABSTRACTTissue engineering is a vast expanding field with applications in areas such as tissue/organ transplantation, drug delivery, in vitro models, and so on. Biomaterials form an essential component in tissue engineering by acting as a template for cellular activity, therefore, novel tissue‐engineered biomaterials with innovative properties are in high demand. Hence, this work proposes an interpenetrating polymer network cryogel of chitosan and hydroxyethyl methacrylate (HEMA) as a tissue‐engineered biomaterial with uniform and high cell seeding throughout the cryogel matrix. The physical analysis of the cryogels demonstrated a highly macroporous structure exhibiting uniform pore size distribution and overall porosity through field emission‐scanning electron microscopy (FE‐SEM) analysis with pore sizes lying in the range of 50–200 μm and 150–400 μm in horizontal and transverse plane, respectively. The cryogels were also found to be degradable with an average percent degradation of 17.28 ± 1.47% in 4 weeks, and their mechanical properties revealed an average compressive strength of 0.05 MPa and an elastic modulus of 3 MPa. Further, biological characterization of the cryogel through direct contact test depicted an excellent biocompatibility with L929 mouse fibroblast and MC3T3‐E1 preosteoblasts with negligible presence of dead cells in and around the cryogel. Uniform and high cell seeding with an increasing proliferation trend of MC3T3‐E1 cells was observed on these cryogels through live‐dead staining and MTT assay at day 1, 3, and 7‐time point. Cell adherence studies via FE‐SEM analysis also depicted a similar trend with uniform MC3T3‐E1 cell distribution along with highly flattened morphology and extracellular matrix production. Therefore, based on their promising physico‐chemical and biological properties, HEMA‐Chitosan cryogels exhibit a strong potential application as tissue engineered biomaterials.
Synthesis of a Novel Derivative Containing Phosphaphenanthrene and Phenyl Propargyl Ether Groups Toward Enhanced Flame Retardance and Mechanical Properties of Epoxy Resin
Authors: Luo Qinqin , Sun Yulin 
Published: January 2025

ABSTRACTTo achieve effective flame retardancy and improved toughness in epoxy resin (EP) without compromising strength and modulus, a new derivative containing phosphaphenanthrene and phenyl propyl ether groups (HPMD) has been developed and used as an additive. The structure of HPMD has been fully characterized, and the properties of HPMD and modified EP have been thoroughly studied. The phenyl propyl ether group in HPMD can undergo Claisen type rearrangement to form benzopyran during heat treatment and subsequently polymerize by curing via the CC bond in the benzopyran. The unique rigid and flexible structures of HPMD allow it to disperse in the EP matrix through melting. Dynamic mechanical analysis suggests that HPMD has a high degree of interpenetration in the EP matrix, resulting in a single‐phase structure for the epoxy resins containing HPMD. Notably, incorporating only 4 wt% HPMD content into the polymer matrix yields a flame‐retarded, toughened, and strengthened EP. EP containing 4 wt% HPMD (EP4; phosphorus content of only 0.33%) displays a limiting oxygen index value of 31.1% and exhibits a UL94 V‐0 rating. Compared with that of EP, the combustion of EP4 exhibits a decrease in the peak of heat release rate, maximum average heat rate emission, average heat release rate, and total heat release, along with an increase in the char yield, further indicating that HPMD effectively retards the combustion of EP. Moreover, EP4 displays improvements in tensile strength, elongation at break, flexural strength, flexural modulus, and impact strength of 18.4%, 9.7%, 14.9%, 19.1%, and 26.7%, respectively, compared with unmodified EP. Analysis of HPMD's flame‐retardant action suggests that it exhibits flame‐retardant activity simultaneously in the gas and condensed phases. Significant progress has been demonstrated toward the development of a meltable epoxy resin additive that combines flame‐retardant, toughening, and strengthening functions while avoiding phase separation from the epoxy resin.
Characterizing the Profile of Anhedonia in Individuals With Schizotypal Traits, Subthreshold Depression and Autistic Traits
Authors: Wang Ling‐ling , Gao Yan , Yan Chao , Hu Hui‐xin , Lui Simon S. Y. , Wang Yi , Chan Raymond C. K. 
Published: January 2025

ABSTRACTAnhedonia is believed to be transdiagnostic symptom exist in various disorders including schizophrenia, major depressive disorder, and autism spectrum disorder. However, very few studies attempted to profile subclinical samples with schizophrenia, depressive, and autistic symptoms using measures of anhedonia scales. This study adopted a cluster analytical approach to examine the anhedonia profile in 46 individuals with schizotypal trait (ST), 43 subthreshold depression (SD), 27 autistic trait (AT), and 41 healthy controls. They completed a set of checklists capturing different dimensions of anhedonia including the anticipatory and consummatory interpersonal pleasure scale, the temporal experience of pleasure scale, the motivation and pleasure scale and the belief about pleasure scale. Cluster analysis was conducted on these measures among the merged sample of ST, SD, and AT. To validate the clusters, we administered measures on nonsocial reward processing, self‐reported empathy, and social functioning. A three‐cluster solution was found to be the best fit. Cluster 1 (n = 48) showed high pleasure experience, motivation, and belief about pleasure and spread evenly across three groups. Cluster 2 (n = 31) was characterized by low levels of anticipatory and consummatory pleasure specifically for the social domain, largely comprised of individuals with ST. Cluster 3 (n = 37) showed low levels of consummatory pleasure, motivation, and belief about pleasure, largely comprised of individuals with SD. The resultant clusters differed in social process and functioning. The current findings suggested distinct anhedonia subtypes within different subclinical populations. These findings may have implications for early detection and prevention for anhedonia.
The Impact of Age and Sex on the Expression and Severity of Borderline Symptoms in Individuals With Borderline Personality Disorder: An Iranian Study
Authors: Mohajerin Banafsheh , Howard Richard Charles 
Published: January 2025

ABSTRACTReports of sex and age differences in the presentation of borderline symptoms have been limited to the Western literature and have not systematically compared adolescents with emerging and older adults with borderline personality disorder (BPD). This study aimed to examine the impact of age and sex on the expression of borderline symptoms in adolescents, young adults, and older adults with BPD. A sample of 493 Iranian individuals with a confirmed diagnosis of BPD was segregated into 2 age groups: 134 young people aged 12–25 (mean = 17.60), and 142 adults aged 26 and older (mean = 31.69). Young people were divided into: 241 adolescents (mean = 15.59) and 100 young adults (mean = 22.45). Older groups compared with their younger counterparts showed significantly greater severity of borderline symptoms and greater difficulties with emotion regulation, but younger groups showed greater emotional and behavioral dysregulation. Sex differences generally mirrored those reported in the Western literature. Despite being limited by its cross‐sectional design, this study suggests that adolescents, young adults, and older adults differ significantly in the way their borderline symptoms are expressed. The development of gender identity in adolescents with BPD and a disturbed sense of self is a neglected but potentially fruitful area of future research.