bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selecting implementation strategies to improve implementation of integrated PrEP for pregnant and postpartum populations in Kenya: a sequential explanatory mixed methods analysis
Authors: Hicks Sarah , Odhiambo Ben , Abuna Felix , Dettinger Julia C. , Ngumbau Nancy , Gómez Laurén , Sila Joseph , Oketch George , Sifuna Enock , Weiner Bryan J. , John-Stewart Grace , Kinuthia John , Wagner Anjuli D. 
Published: August 2023

Abstract
Background
There is a higher risk for HIV acquisition during pregnancy and postpartum. Pre-exposure prophylaxis (PrEP) is recommended during this period for those at high risk of infection; integrated delivery in maternal and child health (MCH) clinics is feasible and acceptable but requires implementation optimization.

Methods
The PrEP in Pregnancy, Accelerating Reach and Efficiency study (PrEPARE; NCT04712994) engaged stakeholders to prioritize determinants of PrEP delivery (using Likert scores) and prioritize PrEP delivery implementation strategies. Using a sequential explanatory mixed methods design, we conducted quantitative surveys with healthcare workers at 55 facilities in Western Kenya and a stakeholder workshop (including nurses, pharmacists, counselors, and county and national policymakers), yielding visual plots of stakeholders’ perceived feasibility and effectiveness of the strategies. A stepwise elimination process was used to identify seven strategies for empirical testing. Facilitator debriefing reports from the workshop were used to qualitatively assess the decision-making process.

Results
Among 146 healthcare workers, the strongest reported barriers to PrEP delivery were insufficient providers and inadequate training, insufficient space, and high volume of patients. Sixteen strategies were assessed, 14 of which were included in the final analysis. Among rankings from 182 healthcare workers and 44 PrEP policymakers and implementers, seven strategies were eliminated based on low post-workshop ranking scores (bottom 50th percentile) or being perceived as low feasibility or low effectiveness for at least 50% of the workshop groups. The top seven strategies included delivering PrEP within MCH clinics instead of pharmacies, fast-tracking PrEP clients to reduce waiting time, delivering PrEP-related health talks in waiting bays, task shifting PrEP counseling, task shifting PrEP risk assessments, training different providers to deliver PrEP, and retraining providers on PrEP delivery. All top seven ranked strategies were grouped into bundles for subsequent testing. Facilitator debriefing reports generally aligned with rankings but noted how stakeholders’ decision-making changed when considering the impact of strategies on facility staff and non-PrEP clients.

Conclusions
The most impactful barriers to integrated PrEP delivery in MCH clinics were insufficient staffing and space. Implementation strategies prioritized through multiple methods of stakeholder input focused on co-location of services and increasing clinic efficiency. Future testing of these stakeholder-prioritized strategy bundles will be conducted to assess the effectiveness and implementation outcomes.
Rapid and visual RPA-CRISPR/Cas12a detection for Staphylococcus pseudintermedius and methicillin-resistant S. pseudintermedius in clinical samples of dogs and cats
Authors: Gao Pingping , Shi Shuobo , Zhang Di , Zhang Yueping 
Published: August 2023

AbstractStaphylococcus pseudintermedius can cause severe infections of the skin, ear and other tissues in dogs and cats. Methicillin-resistant S. pseudintermedius (MRSP) has recently become more prevalent, posing a severe threat to companion animals and public health. Therefore, rapid and accurate diagnosis of S. pseudintermedius and MRSP infections in dogs and cats is essential for timely controlling infections. The development of CRISPR/Cas technology offers an innovative solution for rapid diagnosis. Here, we established an assay combining recombinant polymerase amplification (RPA) and CRISPR/Cas12a. By separately detecting the spsJ gene, the specific gene of S. pseudintermedius, and the mecA gene, the methicillin resistance gene, this method allows for the direct detection of methicillin-susceptible S. pseudintermedius (MSSP) and MRSP in clinical samples at 37 °C for a total of 40 min, The results can be directly visualized by the naked eye under blue light. The limits of detection of the RPA-CRISPR/Cas12a assay were 103 copies per reaction for the spsJ gene and 104 copies per reaction for the mecA gene. The RPA-CRISPR/Cas12a detection successfully detected and differentiated clinical isolates of MSSP and MRSP without cross-reactivity with other tested bacteria species. The evaluation of the detection performance of RPA-CRISPR/Cas12a with 47 clinical samples (without culture) from dogs and cats showed that the results of detection were 100% consistent with those of clinical culture and colony sequencing, which was more sensitive than PCR. RPA-CRISPR/Cas12a assay can quickly and sensitively detect S. pseudintermedius and MRSP in clinical samples without expensive instruments, making it suitable for small veterinary clinics.
Passionate Encounters, Public Healing
Authors: Wurtzel Ellen 
Published: August 2023

AbstractThis article focuses on the resurgence of urban bathhouses (called estuves in French after the stoves that heated them) between the thirteenth and sixteenth centuries in Paris and other northern French-speaking cities. Popular and widespread institutions, bathhouses contributed to both individual well-being and civic health in cities across the kingdom. Using medical treatises, trial records, literary sources, and archival documentation, the article argues that bathhouses encouraged sociability, brought disparate groups together, and were in fact essential to the circulation and well-being of people in medieval cities as places of emotional community.
Unequal Duties and Unequal Retirement: Decomposing the Women's Labor Force Decline in Postreform China
Authors: Feng Kai 
Published: August 2023

Abstract
The gender gap in labor force participation (LFP) in China has grown over the last 30 years, despite substantial advances in women's education and economic development. Previous research has identified gender discrimination and work–family conflicts as two key explanations for the gap, both of which have risen since the start of China's economic reform in 1978. Using multiple waves of the national census and household panel data from China, this research shows that one overlooked mechanism widening the LFP gender gap lies in the institutional constraints that require women to retire earlier than men. This research also demonstrates how the impact of women's early retirement on the LFP gender gap has been exacerbated by two societal-level changes: (1) population aging, which increased the share of women who reached the retirement age; and (2) economic development, which increased the number of women entering nonfarming occupations and, hence, the gender-based retirement system. These findings suggest that without significant revisions to China's retirement system, the LFP gender gap will continue to expand as the population ages and economic development proceeds.
Peritrigeminal Safe Entry Zone Access to Anterolateral Pons Using the Presigmoid Retrolabyrinthine Suprameatal Approach: A Cadaveric Morphometric Study
Authors: Hoz Samer S. , Sharma Mayur , Palmisciano Paolo , Johnson Mark D. , Ismail Mustafa , Muthana Ahmed , Al-Ageely Teeba A. , Forbes Jonathan A. , Prestigiacomo Charles J. , Zuccarello Mario , Andaluz Norberto 
Published: August 2023

BACKGROUND AND OBJECTIVES:
Access to the anterolateral pontine lesions can be achieved through the peritrigeminal and supratrigeminal safe entry zones using Kawase, retrosigmoid, or translabyrinthine approaches. However, these approaches entail shallow extensive dissection, tangential access, and compromise vestibulocochlear function. We aimed to investigate infratentorial presigmoid retrolabyrinthine approach to access pontine lesions through the peritrigeminal zone.

METHODS:
We performed 10 presigmoid retrolabyrinthine suprameatal approach dissections in 5 cadaveric heads. Anatomic-radiological characteristics and variations were evaluated. Six morphometric parameters were measured and analyzed to predict surgical accessibility.

RESULTS:
The pontine infratrigeminal area was accessible in all patients. The mean exposed area of the anterolateral pontine surface was 98.95 cm2 (±38.11 cm2). The mean length of the exposed trigeminal nerve was 7.9 cm (±2.9 cm). Preoperative anatomic-radiological parameters may allow to select patients with favorable anatomy that offers appropriate surgical accessibility to the anterior pontine cavernoma through a presigmoid retrolabyrinthine corridor.

CONCLUSION:
Anterolateral pontine lesions can be accessed through a minimally invasive infratentorial presigmoid retrolabyrinthine approach by targeting the infratrigeminal safe entry zone. Further clinical studies should be conducted to evaluate the viability of this technique for treating these complex pathologies in real clinical settings.
Intrinsic and extrinsic regulation of rhabdomyolysis susceptibility by Tango2
Authors: Kim Euri S. , Casey Jennifer G. , Tao Brian S. , Mansur Arian , Mathiyalagan Nishanthi , Wallace E. Diane , Ehrmann Brandie M. , Gupta Vandana A. 
Published: August 2023

ABSTRACT
Rhabdomyolysis is a clinical emergency characterized by severe muscle damage, resulting in the release of intracellular muscle components, which leads to myoglobinuria and, in severe cases, acute kidney failure. Rhabdomyolysis is caused by genetic factors linked to increased disease susceptibility in response to extrinsic triggers. Recessive mutations in TANGO2 result in episodic rhabdomyolysis, metabolic crises, encephalopathy and cardiac arrhythmia. The underlying mechanism contributing to disease onset in response to specific triggers remains unclear. To address these challenges, we created a zebrafish model of Tango2 deficiency. Here, we demonstrate that the loss of Tango2 in zebrafish results in growth defects, early lethality and increased susceptibility of skeletal muscle defects in response to extrinsic triggers, similar to TANGO2-deficient patients. Using lipidomics, we identified alterations in the glycerolipid pathway in tango2 mutants, which is critical for membrane stability and energy balance. Therefore, these studies provide insight into key disease processes in Tango2 deficiency and have increased our understanding of the impacts of specific defects on predisposition to environmental triggers in TANGO2-related disorders.
Antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin on Enterococcus faecalis: A confocal laser scanning microscopic study
Authors: De Sarkar Madhureema , Mala Kundabala , Shenoy Mala Suchitra , Kabekkodu Shama Prasada , Natarajan Srikant , Shetty Neeta , Madhav Kamath Priyanka , Thomas Manuel 
Published: August 2023

Background: Sealers with antimicrobial properties play an important role in endodontic therapy success especially against Enterococcus faecalis infection found in failed root canal therapy. Addition of antibiotic agents to endodontic sealers may show significant increase in their antibacterial properties both against anaerobic and aerobic microbes. The purpose of the present study was to evaluate antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin against E. faecalis and post-root canal treatment viability of Enterococcus faecalis on the first and seventh day. Methods: A total of 60 extracted human mandibular premolar teeth were decoronated after initial decontamination with 1% NaOCl. Root length standardized to 12 mm. Canal instrumentation was done using ProTaper Universal file system till size F2 using 5.25% NaOCl. It was then infected with a pure strain of E. faecalis for a period of four days. Obturation was done using plain sealer, (n=30) and sealer-antibiotic combination, (n=30). Half of the teeth were sectioned at 24 hours (S, SA) and other half were sectioned seven days after obturation (S7, SA7). All samples were stained with SYTO9 and propidium iodide for imaging under Confocal Laser Scanning microscope. Statistical analysis was performed with the statistical software SPSS v. 17.0 (SPSS for Windows; SPSS Inc, Chicago, IL). Data was analysed using One Way ANOVA and post hoc Tukey test to determine statistical significance with p value < 0.01 considered significant. Results: Statistically significant differences were observed in green to red ratio between group S (9.561976) and S7 (0.435418) (p < 0.01). There was no difference found between SA (mean of green to red ratio, (0.70431) and SA7 (mean of green to red ratio, 0.85184). Conclusions: Antibiotics added to the sealer effectively eradicated of E. faecalis 24 hours post-obturation. However, after seven days, plain sealer was as effective as sealer-antibiotic combination.
Adult malaria mortality during 2019 at Bo Government Hospital, Sierra Leone
Authors: Kpagoi Satta Sylvia T.K. , Aimone Ashley , Ansumana Rashid , Swaray Ibrahim , Gelband Hellen , Eikelboom John W. , Jha Prabhat , Bogoch Isaac I. 
Published: August 2023

It is uncertain whether malaria is an important cause of death among adults in endemic areas. We performed a chart review of adults admitted to Bo Government Hospital during 2019. Of 893 admissions, 149 (59% female, mean age 58.5 years) had a laboratory diagnosis of malaria and 22 (14.8%) died. Mortality was significantly higher among patients with severe malaria compared with those who had non-severe malaria (6/20 [30%] versus 16/129 [12.4%], p=0.031). Our results suggest that malaria is a common cause of death in hospitalized Sierra Leonian adults.
Application of halotolerant Azotobacter chroococcum W4ii isolated from technosoils to mitigate salt stress in wheat plant
Authors: Binod Kumar Sweta , Kalwasińska Agnieszka , Swiontek Brzezinska Maria , Wróbel Monika 
Published: August 2023

Background: Technosoils in Inowrocław, central Poland, are impacted by human activities and exhibit high salinity (ECe up to 70 dS/m) due to a soda lime repository. These saline environments pose challenges to plant growth and soil health. However, they also offer an opportunity for the evolution of microorganisms adapted to such conditions, including plant growth-promoting rhizospheric (PGPR) bacteria. The hypothesis tested here was that introducing PGPR bacteria from these environments could boost degraded soil performance, leading to better plant biomass and improved pathogen defense. Methods: Azotobacter chroococcum W4ii was isolated from the rhizosphere of wheat (Triticum aestivum L.) for its plant growth properties on wheat plants under salt stress. Results: Wheat seeds co-inoculated with A. chroococcum W4ii under 200 mM salt stress showed significant improvement in various growth parameters such as seeds germination (by 130%), shoot biomass (15%), chlorophyll b content (40%) compared to un-inoculated ones. Bacterial inoculation decreased the level of malondialdehyde (MDA), whereas it elevated the antioxidative enzymatic activities of peroxidase (POD). The test isolate also enhanced the level of defense enzymes like β-1, 3-glucanase, which can protect plants from infection by pathogens. The bacterium could also successfully colonize the wheat plants. Conclusions: These results indicate that A. chroococcum isolated from the technosoils has the potential to promote wheat growth under salt stress and can be further used as a bioinoculant in the salt affected agricultural fields.
A New Family of Route Formulations for Split Delivery Vehicle Routing Problems
Authors: Balster Isaac , Bulhões Teobaldo , Munari Pedro , Pessoa Artur Alves , Sadykov Ruslan 
Published: August 2023

We propose a new family of formulations with route-based variables for the split delivery vehicle routing problem with and without time windows. Each formulation in this family is characterized by the maximum number of different demand quantities that can be delivered to a customer during a vehicle visit. As opposed to previous formulations in the literature, the exact delivery quantities are not always explicitly known in this new family. The validity of these formulations is ensured by an exponential set of nonrobust constraints. Additionally, we explore a property of optimal solutions that enables us to determine a minimum delivery quantity based on customer demand and vehicle capacity, and this number is often greater than one. We use this property to reduce the number of possible delivery quantities in our formulations, improving the solution times of the computationally strongest formulation in the family. Furthermore, we propose new variants of nonrobust cutting planes that strengthen the formulations, namely limited-memory subset-row covering inequalities and limited-memory strong k-path inequalities. Finally, we develop a branch-cut-and-price (BCP) algorithm to solve our formulations enriched with the proposed valid inequalities, which resorts to state-of-the-art algorithmic enhancements. We show how to effectively manage the nonrobust cuts when solving the pricing problem that dynamically generates route variables. Numerical results indicate that our formulations and BCP algorithm establish new state-of-the-art results for the variant with time windows, as many benchmark instances with 50 and 100 customers are solved to optimality for the first time. Several instances of the variant without time windows are solved to proven optimality for the first time. Funding: This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico [Grants 306033/2019-4, 313220/2020-4, and 314088/2021-0], the Région Nouvelle Aquitaine, France [Grant AAPR2020A-2020-8601810], the Agence Nationale de la Recherche [Grant ANR-20-CE40-0021-01], the Fundação de Amparo à Pesquisa do Estado de São Paulo [Grants 13/07375-0, 16/01860-1, and 19/23596-2], and the Paraíba State Research Foundation [Grants 261/2020 and 041/2023]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/trsc.2022.0085 .
Ultracompact parabolic sidewall-profiled hybrid plasmonic waveguide Bragg grating with optimized spectral properties of long-wavelength passband
Authors: Chi Tiantian , Xu Ji , Yang Le , Wang Jun , Li Sheng , Yao Han , Cheng Huichao , Zhang Baifu , Lu Yunqing , Liu Ning 
Published: August 2023

An ultracompact hybrid plasmonic waveguide Bragg grating (HPWBG) with improved spectral properties of long-wavelength passband is proposed. A hollow HPW is introduced to suppress the entire loss, and a parabolic profiled sidewall is designed to optimize the spectral properties for specific wave bands. The transfer matrix method and finite element method are combined to ensure the efficiency of numerical research. The results show that the parabolic profile effectively reduces the reflection and strengthens the resonance of the mode in the long-wavelength passband, suppressing the oscillations and realizing significant smoothness and improvement in transmission. The optimized transmittance is greater than 99%, and insertion loss is as low as 0.017 dB. A wide bandgap of 103 nm is also attained. The structure also has a compactness with a length of 3.4 µm and exhibits good tolerance. This work provides a scheme for designing and optimizing wavelength selecting devices and has potential application value in integrated photonic devices.
First report on mycetoma in Turkana County—North-western Kenya
Authors: Colom María Francisca , Ferrer Consuelo , Ekai John Lochuke , Ferrández David , Ramírez Laura , Gómez-Sánchez Noelia , Leting Simion , Hernández Carmen 
Published: August 2023

Mycetoma is one of the six Neglected Tropical Diseases that are prevalent in Turkana County (northwest Kenya). The aim of the study was to estimate the prevalence of mycetoma in the county, as well as to describe the main causative agents involved in the disease using methods affordable locally. Based on the data collected by the team of cooperative medicine Cirugia en Turkana (Surgery in Turkana), a specific study for mycetoma was started during the 16th humanitarian medicine campaign in February 2019. Patients with suspected mycetoma were studied at the Lodwar County Referral Hospital (LCRH). After informing the patient and getting their consent, the lesions were examined and sampled (mainly by biopsy) and clinical data were recorded. Samples were washed in sterile saline solution and cut in fragments. Some of these were inoculated on Sabouraud Dextrose Agar, Malt Extract Agar, and diluted Nutrient Agar plates. One fragment of each sample was used for DNA extraction. The DNA and the rest of the fragments of samples were kept at -20°C. All cultures were incubated at room temperature at the LCRH laboratory. The DNA obtained from clinical samples was submitted to PCR amplification of the ITS-5.8S and the V4-V5 16S rRNA gene region, for the detection and identification of fungi and bacteria respectively.
From February 2019 till February 2022, 60 patients were studied. Most of them were men (43, 74,1%) between 13 and 78 y.o. (mean age 37). Half of the patients were herdsmen but, among women 40% (6) were housewives and 26.7% (4) charcoal burners. Lesions were mainly located at the feet (87.9%) and most of the patients (54; 93.1%) reported discharge of grains in the exudate, being 27 (46.6%) yellow or pale colored and 19 (32.8%) of them dark grains.
Culture of clinical samples yielded 35 fungal and bacterial putative causative agents. Culture and molecular methods allowed the identification of a total of 21 causative agents of mycetoma (39.6% of cases studied). Most of them (17) corresponded to fungi causing eumycetoma (80.9%) being the most prevalent the genus Madurella (7; 41.2%), with two species involved (M. mycetomatis and M. fahalii), followed by Aspergillus (2; 11.8%). Other minority genera detected were Cladosporium, Fusarium, Acremonium, Penicillium, and Trichophyton (5.9% each of them). Actinobacteria were detected in 19.1% of samples, but only Streptomyces somaliensis was identified as a known agent of mycetoma, the rest being actinobacteria not previously described as causative agents of the disease, such as Cellulosimicrobium cellulans detected in two of the patients.
Although Kenya is geographically located in the mycetoma belt, to our knowledge this is the first report on mycetoma in this country from 1973, and the first one for Turkana County.