bluuebunny/crossref_metadata_2025_split huggingface dataset:
A scoping review and behavioural analysis of factors underlying overuse of antimicrobials
Authors: Poluektova Olga , Robertson Deirdre A , Rafferty Aisling , Cunney Robert , Lunn Peter D 
Published: May 2023

Abstract

Background
Overuse of antimicrobials is a challenging global issue that contributes to antimicrobial resistance. Despite widespread awareness of the problem among members of the medical community and various attempts to improve prescription practices, existing antimicrobial stewardship programmes are not always effective. In our view, this may reflect limited understanding of factors that influence prescription of antimicrobials as empirical therapy, implying a need to address the psychological mechanisms behind some of the specific behaviours involved.

Objectives
To identify factors that influence the antimicrobials prescription as empirical therapy, and to relate these factors to findings from behavioural science.

Methods
We conducted a scoping review of the literature on the factors underlying antimicrobial prescription decisions, following the protocol designed using PRISMA guidelines.

Results and conclusions
From a final sample of 90 sources, we identified ten factors important in antimicrobial prescription decisions. In the second stage of our analysis, we grouped them into five final categories: (1) nature of the decision, (2) social influences, (3) individual differences, (4) characteristics of the patient, (5) context. We analyse these categories using a behavioural science perspective.
Comparing the effectiveness of different insecticide application orders for suppressing Drosophila suzukii Matsumura (Diptera: Drosophilidae) infestation: experimental and modeling approaches
Authors: Mermer Serhan , Rossi Stacconi Marco Valerio , Tait Gabriella , Pfab Ferdinand , Sial Ashfaq A , Disi Joseph O , Burrack Hannah J , Toennisson Aurora , Xue Lan , Zhang Chengzhu , Walton Vaughn M 
Published: May 2023

Abstract
Drosophila suzukii Matsumura (Diptera: Drosophilidae) is a key pest of soft-skinned fruit such as blackberry and blueberry. Differing seasonal spray regimes are expected to have variable effects on D. suzukii populations. Semi-field cage trials were performed at three locations in the United States (Georgia, Oregon, and North Carolina) on blueberry and blackberry crops to evaluate this hypothesis. Insecticides with different efficacy rates (ZC - zeta-cypermethrin, SPI - spinetoram, CYAN - cyantraniliprole) were applied during field experiments conducted within large cages. Treatment schedules consisted of two insecticide applications which performed over three weeks. Seasonal treatment schedules were applied in the following order: ZC-CYAN and CYAN-ZC in rabbiteye and highbush blueberry with the addition of a ZC-SPI treatment applied in blackberry. In addition, a population model was applied to simulate the relative efficacy of the insecticide schedules in Oregon on D. suzukii population model based on previously published efficacy, biological, and weather parameters. Overall, all schedules resulted in reduced D. suzukii infestation compared to untreated control (UTC) treatments, with statistical differences in all three locations. The numerically lower infestation was found in some cases in ZC-CYAN schedule. Population modeling conducted exclusively for blueberry, and the simulations indicated no discernible differences between the two respective schedules (ZC-CYAN vs CYAN-ZC). The present study demonstrates that seasonal infestation of D. suzukii could be suppressed irrespective of application order. Additional research is required to assess the optimal timing and sequence of insecticide applications for controlling seasonal populations of D. suzukii in fruit crops. Such information could be invaluable for growers who are seeking to strategize their insecticide applications.
Spatiotemporal distribution of Borrelia miyamotoi (Spirochaetales: Spirochaetaceae) and coinfection with other tick-borne pathogens in host-seeking Ixodes scapularis (Acari: Ixodidae) from New York State, USA
Authors: Foley Nicole , O’Connor Collin , Falco Richard C , Vinci Vanessa , Oliver JoAnne , Haight Jamie , Sporn Lee Ann , Harrington Laura , Mader Emily , Wroblewski Danielle , Backenson P Bryon , Prusinski Melissa A 
Published: May 2023

Abstract
Blacklegged ticks (Ixodes scapularis Say, Acari: Ixodidae) were collected from 432 locations across New York State (NYS) during the summer and autumn of 2015–2020 to determine the prevalence and geographic distribution of Borrelia miyamotoi (Spirochaetales: Spirochaetaceae) and coinfections with other tick-borne pathogens. A total of 48,386 I. scapularis were individually analyzed using a multiplex real-time polymerase chain reaction assay to simultaneously detect the presence of Bo. miyamotoi, Borrelia burgdorferi (Spirochaetales: Spirochaetaceae), Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae), and Babesia microti (Piroplasmida: Babesiidae). Overall prevalence of Bo. miyamotoi in host-seeking nymphs and adults varied geographically and temporally at the regional level. The rate of polymicrobial infection in Bo. miyamotoi-infected ticks varied by developmental stage, with certain co-infections occurring more frequently than expected by chance. Entomological risk of exposure to Bo. miyamotoi-infected nymphal and adult ticks (entomological risk index [ERI]) across NYS regions in relation to human cases of Bo. miyamotoi disease identified during the study period demonstrated spatial and temporal variation. The relationship between select environmental factors and Bo. miyamotoi ERI was explored using generalized linear mixed effects models, resulting in different factors significantly impacting ERI for nymphs and adult ticks. These results can inform estimates of Bo. miyamotoi disease risk and further our understanding of Bo. miyamotoi ecological dynamics in regions where this pathogen is known to occur.
A kernel Stein test for comparing latent variable models
Authors: Kanagawa Heishiro , Jitkrittum Wittawat , Mackey Lester , Fukumizu Kenji , Gretton Arthur 
Published: May 2023

AbstractWe propose a kernel-based nonparametric test of relative goodness of fit, where the goal is to compare two models, both of which may have unobserved latent variables, such that the marginal distribution of the observed variables is intractable. The proposed test generalizes the recently proposed kernel Stein discrepancy (KSD) tests (Liu et al., Proceedings of the 33rd international conference on machine learning (pp. 276–284); Chwialkowski et al., (2016), In Proceedings of the 33rd international conference on machine learning (pp. 2606–2615); Yang et al., (2018), In Proceedings of the 35th international conference on machine learning (pp. 5561–5570)) to the case of latent variable models, a much more general class than the fully observed models treated previously. The new test, with a properly calibrated threshold, has a well-controlled type-I error. In the case of certain models with low-dimensional latent structures and high-dimensional observations, our test significantly outperforms the relative maximum mean discrepancy test, which is based on samples from the models and does not exploit the latent structure.
Population divergence in heat and drought responses of a coastal plant: from metabolic phenotypes to plant morphology and growth
Authors: Schrieber Karin , Glüsing Svea , Peters Lisa , Eichert Beke , Althoff Merle , Schwarz Karin , Erfmeier Alexandra , Demetrowitsch Tobias 
Published: May 2023

Abstract
Studying intraspecific variation in multistress responses is central for predicting and managing the population dynamics of wild plant species under rapid global change. Yet, it remains a challenging goal in this field to integrate knowledge on the complex biochemical underpinnings for the targeted ‘non-model’ species. Here, we studied divergence in combined drought and heat responses among Northern and Southern European populations of the dune plant Cakile maritima, by combining comprehensive plant phenotyping with metabolic profiling via FT-ICR-MS and UPLC-TQ-MS/MS. We observed pronounced constitutive divergence in growth phenology, leaf functional traits, and defence chemistry (glucosinolates and alkaloids) among population origins. Most importantly, the magnitude of growth reduction under drought was partly weaker in southern plants and associated with divergence in plastic growth responses (leaf abscission) and the modulation of primary and specialized metabolites with known central functions not only in plant abiotic but also in biotic stress responses. Our study indicates that divergent selection has shaped the constitutive and drought-/heat-induced expression of numerous morphological and biochemical functional traits to mediate higher abiotic stress resistance in southern Cakile populations, and highlights that metabolomics can be a powerful tool to explore the underlying mechanisms of local adaptation in ‘non-model’ species.
Sulfide promotes tolerance to drought through protein persulfidation in Arabidopsis
Authors: Jurado-Flores Ana , Aroca Angeles , Romero Luis C , Gotor Cecilia 
Published: May 2023

Abstract
Hydrogen sulfide (H2S) is a signaling molecule that regulates essential plant processes. In this study, the role of H2S during drought was analysed, focusing on the underlying mechanism. Pretreatments with H2S before imposing drought on plants substantially improved the characteristic stressed phenotypes under drought and decreased the levels of typical biochemical stress markers such as anthocyanin, proline, and hydrogen peroxide. H2S also regulated drought-responsive genes and amino acid metabolism, and repressed drought-induced bulk autophagy and protein ubiquitination, demonstrating the protective effects of H2S pretreatment. Quantitative proteomic analysis identified 887 significantly different persulfidated proteins between control and drought stress plants. Bioinformatic analyses of the proteins more persulfidated in drought revealed that the most enriched biological processes were cellular response to oxidative stress and hydrogen peroxide catabolism. Protein degradation, abiotic stress responses, and the phenylpropanoid pathway were also highlighted, suggesting the importance of persulfidation in coping with drought-induced stress. Our findings emphasize the role of H2S as a promoter of enhanced tolerance to drought, enabling plants to respond more rapidly and efficiently. Furthermore, the main role of protein persulfidation in alleviating reactive oxygen species accumulation and balancing redox homeostasis under drought stress is highlighted.
A framework of biomarkers for brain aging: a consensus statement by the Aging Biomarker Consortium
Authors:   Aging Biomarker Consortium, Jia Yu-Juan , Wang Jun , Ren Jun-Rong , Chan Piu , Chen Shengdi , Chen Xiao-Chun , Chhetri Jagadish K , Guo Junhong , Guo Qihao , Jin Lingjing , Liu Qiang , Liu Qiang , Ma Wenlin , Mao Zhiyong , Song Moshi , Song Weihong , Tang Yi , Wang Difei , Wang Peijun , Xiong Lize , Ye Keqiang , Zhang Junjian , Zhang Weiqi , Zhang Xiaoqing , Zhang Yunwu , Zhang Zhanjun , Zhang Zhuohua , Zheng Jialin , Liu Guang-Hui , Eve Sun Yi , Wang Yan-Jiang , Pei Gang 
Published: May 2023

Abstract
China and the world are facing severe population aging and an increasing burden of age-related diseases. Aging of the brain causes major age-related brain diseases, such as neurodegenerative diseases and stroke. Identifying biomarkers for the effective assessment of brain aging and establishing a brain aging assessment system could facilitate the development of brain aging intervention strategies and the effective prevention and treatment of aging-related brain diseases. Thus, experts from the Aging Biomarker Consortium (ABC) have combined the latest research results and practical experience to recommend brain aging biomarkers and form an expert consensus, aiming to provide a basis for assessing the degree of brain aging and conducting brain-aging-related research with the ultimate goal of improving the brain health of elderly individuals in both China and the world.
Transcription factor bZIP52 modulates Arabidopsis seed oil biosynthesis through interaction with WRINKLED1
Authors: Yang Yuzhou , Kong Que , Tee Wan Ting , Li Yuqing , Low Pui Man , Patra Barunava , Guo Liang , Yuan Ling , Ma Wei 
Published: May 2023

Abstract
Transcriptional regulation mediated by combinatorial interaction of transcription factors (TFs) is a key molecular mechanism modulating plant development and metabolism. Basic leucine zipper (bZIP) TFs play important roles in various plant developmental and physiological processes. However, their involvement in fatty acid biosynthesis is largely unknown. Arabidopsis (Arabidopsis thaliana) WRINKLED1 (WRI1) is a pivotal TF in regulation of plant oil biosynthesis and interacts with other positive and negative regulators. In this study, we identified two bZIP TFs, bZIP21 and bZIP52, as interacting partners of AtWRI1 by yeast-two-hybrid (Y2H)–based screening of an Arabidopsis TF library. We found that coexpression of bZIP52, but not bZIP21, with AtWRI1 reduced AtWRI1-mediated oil biosynthesis in Nicotiana benthamiana leaves. The AtWRI1–bZIP52 interaction was further verified by Y2H, in vitro pull-down, and bimolecular fluorescence complementation assays. Transgenic Arabidopsis plants overexpressing bZIP52 showed reduced seed oil accumulation, while the CRISPR/Cas9-edited bzip52 knockout mutant exhibited increased seed oil accumulation. Further analysis revealed that bZIP52 represses the transcriptional activity of AtWRI1 on the fatty acid biosynthetic gene promoters. Together, our findings suggest that bZIP52 represses fatty acid biosynthesis genes through interaction with AtWRI1, resulting in a reduction of oil production. Our work reports a previously uncharacterized regulatory mechanism that enables fine-tuning of seed oil biosynthesis.
Healthy food traditions? Nutritional quality and food composition of EU geographical indications
Authors: Höhn Gero Laurenz , Huysmans Martijn , Crombez Christophe 
Published: May 2023

Abstract
The EU's ‘Farm to Fork’ strategy aims to promote healthier diets that include more nutritious and natural foods instead of ultra-processed products. Although several producers of geographical indication (GI) foods such as Parma ham advertise their products as artisanal and healthy, little is known yet about whether GIs can contribute to these ambitions. Therefore, we examine the nutritional quality and food composition of GIs compared to non-GIs. We analyse more than 6,000 cheeses and prepared meats marketed in France. We find that in these food categories GIs are associated with lower nutritional quality based on the Nutri-Score metric. Thus, GI regulations may need to facilitate health-driven product reformulations. On the positive side, we find that GIs in the considered dairy and meat categories tend to be less likely to contain food additives and to be ultra-processed. However, this seems to be truer for Protected Designations of Origin than Protected Geographical Indications. Hence, harmonised additive rules could strengthen the natural character of GIs.
Automation and efficiency: A latent class analysis of Irish dairy farms
Authors: Garcia-Covarrubias Luis , Läpple Doris , Dillon Emma , Thorne Fiona 
Published: May 2023

AbstractAutomation has always played a significant role in the productivity and efficiency of agriculture. This paper explores the role of automation on farms' technical efficiency (TE) in an expanding dairy sector. We use a representative sample of Irish dairy farms that includes detailed data on automated technologies. We apply a latent class stochastic frontier model to assess technology heterogeneity amongst Irish dairy farms to obtain their TE scores. We identify two classes of farms: smaller, less intensive and larger, and more intensive farms. We find significant differences between the classes in relation to farm characteristics, input use, labour efficiency, and TE scores. Larger, more intensive farms produce closer to their stochastic frontier. Our findings also suggest that automation has a heterogeneous effect on farms' TE depending on farms' classification. Specifically, adopting automated cluster removers and scrapers is associated with higher TE on smaller, less intensive farms. In contrast, automated parlour feeders are positively associated with larger, more intensive farms' TE. Finally, the implications of adopting automated technologies on Irish farms are discussed.
Qualitative Analysis of the Content Validity of the Virtual Reality Functional Capacity Assessment Tool (VRFCAT) in Schizophrenia: A Multi-Stakeholder Perspective
Authors: Horan William P , Depp Colin A , Hurst Samantha , Linthicum Jared , Vargas Gabriela , Klein Hans , Keefe Richard S E , Harvey Philip D 
Published: May 2023

Abstract
The US Food and Drug Agency (FDA) requires clinical trials targeting cognitive impairment associated with schizophrenia (CIAS) to demonstrate the functional relevance of cognitive improvements by employing a functional co-primary measure. Although quantitative evidence supports the suitability of the Virtual Reality Functional Capacity Assessment Tool (VRFCAT) for this purpose, FDA guidelines for qualification of clinical outcome assessments require evidence of content validity, defined as qualitative evidence that key stakeholders view the measure as relevant and important. To collect this important qualitative data, semi-structured interviews were conducted with outpatients with schizophrenia (n = 24), caregivers (n = 12), and professional peer support specialists (n = 12) to elicit their views about the definition and importance of functional independence, the importance of the functional domains assessed by the VRFCAT (meal planning, using transportation, handling money, shopping), and the relevance of the VRFCAT tasks to these domains. Qualitative thematic analyses revealed consistent themes across groups in defining functional independence, including performing instrumental self-care, financial, and social tasks; making decisions autonomously; and not depending on others to carry out daily activities. There were, however, notable differences in their views regarding the importance of and barriers to functional independence. All groups viewed the VRFCAT as assessing skill domains that are central to independent functioning and, with some minor differences, the VRFCAT tasks were viewed as relevant and meaningful examples of the domains. These qualitative results provide converging evidence that key stakeholders view the VRFCAT as a content-valid measure.
Buen Crédito y Buen Seguro: Legal Status and Restricted Access to Shelter among Low-Income Latina/o Renters in an Immigrant Gateway City
Authors: Schmidt Steven 
Published: May 2023

AbstractSociologists have shown how searches for rental housing reproduce inequalities by race/ethnicity and household income in the United States. Yet scholars know comparatively less about how legal status may also limit access to shelter. To address this gap, this article compares the housing careers of 30 low-income, undocumented/mixed-status, Mexican, Central American, and South American families with those of ten low-income, predominantly Mexican, U.S. citizen/LPR families across 103 total moves in Los Angeles, California. Though citizen and undocumented renters moved for similar reasons, the process of finding a new home varied substantially across these two groups. Renters’ legal status became salient during the screening portion of rental applications, which requested a credit and background check, a verifiable income, and banking information for each household adult. As a result, undocumented renters were excluded from most formal rentals. Instead, these families searched for sympathetic managers or doubled up with friends, family members, and non-kin. Despite these barriers, undocumented and mixed-status families achieved greater housing security over time by transitioning from guests to hosts in doubled up homes. These findings extend prior research on how housing searches stratify movers, the housing careers of Latino immigrant families, and the punitive consequences of illegality.
Postoperative Transfusions after Administration of Delayed Cold-stored Platelets versus Room Temperature Platelets in Cardiac Surgery: A Retrospective Cohort Study
Authors: Klompas Allan M. , Zec Simon , Hanson Andrew C. , Weister Tim , Stubbs James , Kor Daryl J. , Warner Matthew A. 
Published: May 2023

Background
Delayed cold storage of room temperature platelets may extend shelf life from 5 to 14 days. The study hypothesized that the use of delayed cold-stored platelets in cardiac surgery would be associated with decreased postoperative platelet count increments but similar transfusion and clinical outcomes compared to room temperature-stored platelets.

Methods
This is an observational cohort study of adults transfused with platelets intraoperatively during elective cardiac surgery between April 2020 and May 2021. Intraoperative platelets were either room temperature-stored or delayed cold-stored based on blood bank availability rather than clinical features or provider preference. Differences in transfusion and clinical outcomes, including a primary outcome of allogenic transfusion exposure in the first 24 h postoperatively, were compared between groups.

Results
A total of 713 patient encounters were included: 529 (74%) room temperature-stored platelets and 184 (26%) delayed cold-stored platelets. Median (interquartile range) intraoperative platelet volumes were 1 (1 to 2) units in both groups. Patients receiving delayed cold-stored platelets had higher odds of allogeneic transfusion in the first 24 h postoperatively (81 of 184 [44%] vs. 169 of 529 [32%]; adjusted odds ratio, 1.65; 95% CI, 1.13 to 2.39; P = 0.009), including both erythrocytes (65 of 184 [35%] vs. 135 of 529 [26%]; adjusted odds ratio, 1.54; 95% CI, 1.03 to 2.29; P = 0.035) and platelets (48 of 184 [26%] vs. 79 of 529 [15%]; adjusted odds ratio, 1.91; 95% CI, 1.22 to 2.99; P = 0.005). There was no difference in the number of units administered postoperatively among those transfused. Platelet counts were modestly lower in the delayed cold-stored platelet group (−9 × 109/l; 95% CI, −16 to −3]) through the first 3 days postoperatively. There were no significant differences in reoperation for bleeding, postoperative chest tube output, or clinical outcomes.

Conclusions
In adults undergoing cardiac surgery, delayed cold-stored platelets were associated with higher postoperative transfusion utilization and lower platelet counts compared to room temperature-stored platelets without differences in clinical outcomes. The use of delayed cold-stored platelets in this setting may offer a viable alternative when facing critical platelet inventories but is not recommended as a primary transfusion approach.

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