bluuebunny/crossref_metadata_2025_split huggingface dataset:
Levofloxacin HCl-Loaded Eudragit L-Based Solvent Exchange-Induced In Situ Forming Gel Using Monopropylene Glycol as a Solvent for Periodontitis Treatment
Authors: Senarat Setthapong , Tuntarawongsa Sarun , Lertsuphotvanit Nutdanai , Rojviriya Catleya , Phaechamud Thawatchai , Chantadee Takron 
Published: July 2023

Solvent exchange-induced in situ forming gel (ISG) is currently an appealing dosage form for periodontitis treatment via localized injection into the periodontal pocket. This study aims to apply Eudragit L and Eudragit S as matrix components of ISG by using monopropylene glycol as a solvent for loading levofloxacin HCl for periodontitis treatment. The influence of Eudragit concentration was investigated in terms of apparent viscosity, rheological behavior, injectability, gel-forming behavior, and mechanical properties. Eudragit L-based formulation presented less viscosity, was easier to inject, and could form more gel than Eudragit S-based ISG. Levofloxacin HCl-loading diminished the viscosity of Eudragit L-based formulation but did not significantly change the gel formation ability. Higher polymer loading increased viscosity, force-work of injectability, and hardness. SEM photographs and µCT images revealed their scaffold formation, which had a denser topographic structure and less porosity attained owing to higher polymer loading and less in vitro degradation. By tracking with fluorescence dyes, the interface interaction study revealed crucial information such as solvent movement ability and matrix formation of ISG. They prolonged the drug release for 14 days with fickian drug diffusion kinetics and increased the release amount above the MIC against test microbes. The 1% levofloxacin HCl and 15% Eudragit L dissolved in monopropylene glycol (LLM15) was a promising ISG because of its appropriate viscosity (3674.54 ± 188.03 cP) with Newtonian flow, acceptable gel formation and injectability (21.08 ± 1.38 N), hardness (33.81 ± 2.3 N) and prolonged drug release with efficient antimicrobial activities against S. aureus (ATCC 6538, 6532, and 25923), methicillin-resistant S. aureus (MRSA) (S. aureus ATCC 4430), E. coli ATCC 8739, C. albicans ATCC 10231, P. gingivalis ATCC 33277, and A. actinomycetemcomitans ATCC 29522; thus, it is the potential ISG formulation for periodontitis treatment by localized periodontal pocket injection.
Estrategias para la orientación al mercado: El caso de nieves artesanales en Guanajuato, México
Authors: Montiel Flores Julio César , Mendoza Jiménez Gabriela , Valenzuela Juan Pedro 
Published: July 2023

Las nieves o helados en la localidad de Guanajuato, se caracterizan por los sabores exóticos, convirtiéndose en una atracción turística y son parte de su patrimonio cultural. El objetivo del estudio fue determinar la orientación al mercado a través de las actividades de producción y comercialización de microproductores artesanales de nieves en la localidad de Dolores Hidalgo en Guanajuato, México. La metodología empleada fue bajo el diseño de estudio de caso, con enfoque cualitativo, la técnica para la recolección de datos fue la entrevista semiestructurada aplicada a seis productores artesanales ubicados en el Jardín Principal de la localidad. Los informantes se seleccionaron por conveniencia. Los hallazgos destacan que la orientación al mercado en el caso de los fabricantes de nieves se basa en la diferenciación y la producción de sabores exóticos a través de la innovación de los productos como resultado de la comunicación y estrategias elaboradas entre los miembros del negocio familiar.
Using mixture modeling to examine differences in perceptual decision-making as a function of the time and method of participant recruitment
Authors: Ballard Timothy , Evans Nathan J. , Fisher Gina , Sewell David K. 
Published: July 2023

AbstractWe examine whether perceptual decision-making differs as a function of the time in the academic term and whether the participant is an undergraduate participating for course credit, a paid in-person participant, or a paid online participant recruited via Amazon Mechanical Turk. We use a mixture modeling approach within an evidence accumulation framework that separates stimulus-driven responses from contaminant responses, allowing us to distinguish between performance when a participant is engaged in the task and the consistency in this task focus. We first report a survey showing cognitive psychologists expect performance and response caution to be lower among undergraduate participants recruited at the end of the academic term compared to those recruited near the start, and highest among paid in-person participants. The findings from two experiments using common paradigms revealed very little evidence of time-of-semester effects among course credit participants on accuracy, response time, efficiency of information processing (when engaged in the task), caution, and non-decision time, or consistency in task focus. However, paid in-person participants did tend to be more accurate than the other two groups. Groups showed similar effects of speed/accuracy emphasis on response caution and of discrimination difficulty on information processing efficiency, but the effect of speed/accuracy emphasis on information processing efficiency was less consistent among groups. We conclude that online crowdsourcing platforms can provide quality perceptual decision-making data, but recommend that mixture modeling be used to adequately account for data generated by processes other than the psychological phenomena under investigation.
Single-Cell Analysis of CX3CR1+ Cells Reveals a Pathogenic Role for BIRC5+ Myeloid Proliferating Cells Driven by Staphylococcus aureus Leukotoxins
Authors: Loredan Denis G , Devlin Joseph C , Lacey Keenan A , Howard Nina , Chen Ze , Zwack Erin E , Lin Jian-Da , Ruggles Kelly V , Khanna Kamal M , Torres Victor J , Loke P’ng 
Published: July 2023

Abstract
Our previous studies identified a population of stem cell–like proliferating myeloid cells within inflamed tissues that could serve as a reservoir for tissue macrophages to adopt different activation states depending on the microenvironment. By lineage-tracing cells derived from CX3CR1+ precursors in mice during infection and profiling by single-cell RNA sequencing, in this study, we identify a cluster of BIRC5+ myeloid cells that expanded in the liver during chronic infection with either the parasite Schistosoma mansoni or the bacterial pathogen Staphylococcus aureus. In the absence of tissue-damaging toxins, S. aureus infection does not elicit these BIRC5+ cells. Moreover, deletion of BIRC5 from CX3CR1-expressing cells results in improved survival during S. aureus infection. Hence the combination of single-cell RNA sequencing and genetic fate-mapping CX3CR1+ cells revealed a toxin-dependent pathogenic role for BIRC5 in myeloid cells during S. aureus infection.
N-Glycosylation of the Ig Receptors Shapes the Antigen Reactivity in Chronic Lymphocytic Leukemia Subset #201
Authors: Iatrou Anastasia , Gounari Maria , Sofou Electra , Zaragoza-Infante Laura , Markopoulos Ioannis , Sarrigeorgiou Ioannis , Petrakis Georgios , Pechlivanis Nikolaos , Roumeliotou-Dimou Maria , Panayiotidis Panagiotis , Stamatopoulos Basile , Gkanidou Maria , Sandaltzopoulos Rafael , Degano Massimo , Koletsa Triantafyllia , Lymberi Peggy , Psomopoulos Fotis , Ghia Paolo , Agathangelidis Andreas , Chatzidimitriou Anastasia , Stamatopoulos Kostas 
Published: July 2023

Abstract
Subset #201 is a clinically indolent subgroup of patients with chronic lymphocytic leukemia defined by the expression of stereotyped, mutated IGHV4-34/IGLV1-44 BCR Ig. Subset #201 is characterized by recurrent somatic hypermutations (SHMs) that frequently lead to the creation and/or disruption of N-glycosylation sites within the Ig H and L chain variable domains. To understand the relevance of this observation, using next-generation sequencing, we studied how SHM shapes the subclonal architecture of the BCR Ig repertoire in subset #201, particularly focusing on changes in N-glycosylation sites. Moreover, we profiled the Ag reactivity of the clonotypic BCR Ig expressed as rmAbs. We found that almost all analyzed cases from subset #201 carry SHMs potentially affecting N-glycosylation at the clonal and/or subclonal level and obtained evidence for N-glycan occupancy in SHM-induced novel N-glycosylation sites. These particular SHMs impact (auto)antigen recognition, as indicated by differences in Ag reactivity between the authentic rmAbs and germline revertants of SHMs introducing novel N-glycosylation sites in experiments entailing 1) flow cytometry for binding to viable cells, 2) immunohistochemistry against various human tissues, 3) ELISA against microbial Ags, and 4) protein microarrays testing reactivity against multiple autoantigens. On these grounds, N-glycosylation appears as relevant for the natural history of at least a fraction of Ig-mutated chronic lymphocytic leukemia. Moreover, subset #201 emerges as a paradigmatic case for the role of affinity maturation in the evolution of Ag reactivity of the clonotypic BCR Ig.
“DEMONSTRAÇÃO PURAMENTE ANALÍTICA DO TEOREMA QUE ENTRE DOIS VALORES QUAISQUER QUE FORNECEM RESULTADOS DE SINAIS OPOSTOS EXISTE PELO MENOS UMA RAIZ REAL DA EQUAÇÃO”, DE BERNARD BOLZANO
Authors: Carvalho João Bosco Pitombeira de 
Published: July 2023

Apresentamos uma tradução do trabalho acima mencionado de Bernard Bolzano. Assim, o leitor brasileiro terá acesso mais direto a este trabalho importante para a fundamentação da análise matemática em bases puramente aritméticas. Não analisaremos em profundidade o trabalho de Bolzano, reservando isso para oportunidadees posteriores
Session 1 (Part 1) - Making the most of Next Generation Scientists (a panel discussion)
Authors: Knowlton Anne , Ng Carl , Sperschneider Jana , Öpik Maarja , Lennon Sarah 
Published: July 2023

This panel session and Q A will introduce the Next Generation Scientists Symposium, and how you as a delegate can make the most of your time, as well as additional career insights and perspectives. Alistair Hetherington will chair the discussions.
Session 4
Authors: Huong To Thi Mai , Wang Han-Qing 
Published: July 2023

Exploring the hidden half - A surprising role of a plasmodesmal protein controlling the root development in rice under abiotic stressIn plants, the root system plays many important roles, including anchorage, water, and mineral uptake, and responding to environmental signals. The mechanisms underlying root system development are complex and involve many phytohormones of which auxin is the most important one. However, the role of plant stress hormone Jasmonic acid in root development remained elusive. Here, we report that Jasmonic acid promotes crown root development in rice by regulating OsGER4, a Germin-like protein. This gene was discovered by a Genome-Wide Association Study while observing the increase of crown root under Jasmonic acid-stimulated stress. OsGER4 was confirmed to be a hormone-responsive gene involved in various stress responses and its expression coincided with the auxin distribution pattern for lateral root initiation and crown root emergence. Further studies revealed that OsGER4 is targeted in plasmodesmata, suggesting how OsGER4 could be involved in regulating plasmodesmata permeability to regulate the auxin flow as a part of stress responses that results in changes in root development. Overall, the study provides significant novel insights on auxin homeostasis via uncharted roots through plasmodesmata with the involvement of OsGER4 during root formation in rice under perturbation conditions.Rice roots avoid asymmetric heavy metal and salinity stress via a RBOH-ROS-auxin signaling cascadeRoot developmental plasticity is critical for plants to adapt to changing soil environment, where nutrients and abiotic stress factors are distributed heterogeneously. How plant roots sense and avoid heterogeneous abiotic stress in soil remains unclear. Here, we show that, in response to asymmetric stress of heavy metals (Cd, Cu or Pb) and salt (NaCl), rice roots rapidly proliferate lateral root branching in the stress-free area, thereby remodeling root architecture to avoid localized stress. Imaging and quantitative analyses of reactive oxygen species (ROS) show that asymmetric stress induces ROS burst in the tips of the exposed roots, simultaneously triggering rapid systemic ROS signaling to the unexposed roots, which is prerequisite to stress-induced lateral root branching. We identify two respiratory burst oxidase homologs, OsRBOHA and OsRBOHI, as key players for ROS generation and systemic signaling in response to asymmetric stress. Auxin signaling and cell wall remodeling act downstream of the systemic ROS signaling to promote lateral root development. Our study reveals a RBOH-ROS-auxin signaling cascade that enables rice roots to avoid localized stress of heavy metals and salt and provides new insight into root system plasticity in heterogenous soil.
Clinician Interruptions and Patient-Rated Clinician Empathy in Specialty Visits
Authors: Pierson S. Ryan , Lam Ryan , Ngoue Marielle , Rajagopalan Dayal , Ring David , Ramtin Sina 
Published: July 2023

Introduction:
Clinicians tend to interrupt patients when they are describing their problem, which may contribute to feeling unheard or misunderstood. Using transcripts of audio and video recordings from musculoskeletal (MSK) specialty visits, we asked what factors are associated with (1) Perceived clinician empathy, including the time a patient spends describing the problem and time to the first interruption, (2) duration of patient symptom description, and (3) duration between the end of greeting and first nonactive listening interruption.

Methods:
We analyzed transcripts of 194 adult patients seeking MSK specialty care with a median age (Interquartile range [IQR]) of 47 (33 to 59) years. Participants completed postvisit measures of perceived clinician empathy, symptoms of depression, accommodation of pain, and health anxiety. A nonactive listening interruption was defined as the clinician unilaterally redirecting the topic of conversation. Factors associated with patient-rated clinician empathy, patient problem description duration, and time until the first nonactive listening interruption were sought in bivariate and multivariable analyses.

Results:
The patient's narrative was interrupted at least one time in 144 visits (74%). The duration of each visit was a median of 12 minutes (IQR 9 to 16 minutes). The median time patients spent describing their symptoms was 139 seconds before the first interruption (IQR 84 to 225 seconds). The median duration between the end of the initial greeting and the first interruption was 60 seconds (IQR 30 to 103 seconds). Clinician interruption was associated with shorter duration of symptom description. Greater perceived clinician empathy was associated with greater accommodation of pain (regression coefficient [95% confidence interval] = 0.015 [0.0005-0.30]; P = 0.04).

Discussion:
Clinician interruption was associated with shorter symptom presentation, but not with diminished perception of clinician empathy. Although active listening and avoidance of interruption are important communication tactics, other aspects of the patient-clinician relationship may have more effect on patient experience.
Assessment of a Private Payer Bundled Payment Model for Lumbar Decompression Surgery
Authors: Issa Tariq Z. , Lee Yunsoo , Lambrechts Mark J. , Mazmudar Aditya S. , D'Antonio Nicholas D. , Iofredda Patrick , Endersby Kevin , Kalra Andrew , Canseco Jose A. , Hilibrand Alan S. , Vaccaro Alexander R. , Schroeder Gregory D. , Kepler Christopher K. 
Published: July 2023

Introduction:
Although bundled payment models are well-established in Medicare-aged individuals, private insurers are now developing bundled payment plans. The role of these plans in spine surgery has not been evaluated. Our objective was to analyze the performance of a private insurance bundled payment program for lumbar decompression and microdiskectomy.

Methods:
A retrospective review was conducted of all lumbar decompressions in a private payer bundled payment model at a single institution from October 2018 to December 2020. 120-day episode of care cost data were collected and reported as net profit or loss regarding set target prices. A stepwise multivariable linear regression model was developed to measure the effect of patient and surgical factors on net surplus or deficit.

Results:
Overall, 151 of 468 (32.2%) resulted in a deficit. Older patients (58.6 vs. 50.9 years, P < 0.001) with diabetes (25.2% vs. 13.9%, P = 0.004), hypertension (38.4% vs. 28.4%, P = 0.038), heart disease (13.9% vs. 7.57%, P = 0.030), and hyperlipidemia (51.7% vs. 35.6%, P = 0.001) were more likely to experience a loss. Surgically, decompression of more levels (1.91 vs. 1.19, P < 0.001), posterior lumbar decompression (86.8% vs. 56.5%, P < 0.001), and performing surgery at a tertiary hospital (84.8% vs. 70.3%, P < 0.001) were more likely to result in loss. All readmissions resulted in a loss (4.64% vs. 0.0%, P < 0.001). On multivariable regression, microdiskectomy (β: $2,398, P = 0.012) and surgery in a specialty hospital (β: $1,729, P = 0.096) or ambulatory surgery center (β: $3,534, P = 0.055) were associated with cost savings. Increasing number of levels, longer length of stay, active smoking, and history of cancer, dementia, or congestive heart failure were all associated with degree of deficit.

Conclusions:
Preoperatively optimizing comorbidities and using risk stratification to identify those patients who may safely undergo surgery at a facility other than an inpatient hospital may help increase cost savings in a bundled payment model of working-age and Medicare-age individuals.
A multicore emulator with a profiling infrastructure for transactional memory on FPGA
Authors:   , Sönmez Nehir 
Published: July 2023

This thesis attempts to bring together two recent topics by presenting a flexible Transactional Memory environment on a multicore prototype that is realized on FPGA fabric. For this, we devise a MIPS-compatible shared-memory multicore emulator with Hybrid Transactional Memory support, based on the Plasma open source soft processor core. We present the design and implementation of the TMbox system, which features an emulation system of up to 16 soft processor cores interconnected with a bi-directional ring bus, running at 50 MHz on a Virtex5-155t FPGA. Additionally, we build the first comprehensive infrastructure to profile Hybrid TM systems, an extensive visualization environment that enables examining complete transactional executions in detail.

TMbox is a completely modifiable architecture implementing a multicore prototype with support for STM, HTM and Hybrid TM. It was written in various common design languages, and enables modifying the complete stack, down from the ISA, through the software toolchain, up to the well-optimized parallel code. With such an infrastructure, fast execution and quick performance evaluation can be made possible for studies in computer architecture.
En esta tesis se propone juntar dos temas recientes a través de la creación de un sistema de memoria transaccional en un circuito programable (un FPGA). Para ello, ha sido diseñado un emulador de multinúcleo, con soporte para memoria transaccional híbrida y núcleos MIPS basados en el procesador de código libre Plasma. Presentamos el diseño y la implementación del sistema TMbox, que implementa un sistema de emulación de hasta 16 núcleos MIPS interconectados con un bus en anillo bidireccional, funcionando a 50 MHz en un FPGA Virtex5-155t. Adicionalmente, hemos construido la primera infraestructura para perfilado y visualización de memoria transaccional híbrida, que permite ver con un alto detalle ejecuciones transaccionales completas. TMbox es una arquitectura completamente modificable, e implementa un prototipo de un multinúcleo con memoria transaccional en software, hardware e híbrido. Está compuesto de varios lenguajes de diseño muy comunes, y habilita modificar el stack completo, desde la ISA, pasando por el toolchain de software, hasta el código paralelo altamente optimizado. Con una infraestructura como TMbox, es posible la ejecución veloz y la evaluación rápida del rendimiento en investigaciones de arquitectura de computadores.
Resource, data and application management for cloud federations and multi-clouds
Authors:   , Xhagjika Vamis 
Published: July 2023

Distributed Real-Time Media Processing refers to classes of highly distributed, delay no-tolerant applications that account for the majority of the data traffic generated in the world today. Real-Time audio/video conferencing and live content streaming are of particular research interests as technology forecasts predict video traffic surpassing every other type of data traffic in the world in the near future. These applications are very sensitive to both communication properties such as latency, jitter, packet loss, bit rate as well as backend stream processing load profiles. In this work we provide a novel and generalized large-scale Multi-Cloud architectural blueprint for ISP and Carrier providers, that permits smart geo-distributed service placement in order to optimize latency/locality of stream processing applications. We provide as a well self-managed Intra-Cloud federation algorithm based on gradient topologies in order to optimize routes in a live media streaming backend. Additionally we introduce a novel distributed Network Bandwidth Manager that optimizes system stability by arbitrating network bandwidth between multiple Cloud services sharing the same network infrastructure. At last, an empirical study is provided connecting media quality parameters and Cloud backend load profiles, including an algorithm for stream allocation on Cloud Selective Forwarding units.
El procesamiento de medios en tiempo real distribuido se refiere a clases de aplicaciones altamente distribuidas, no tolerantes al retardo, que representan la mayoría del tráfico de datos generado en el mundo actual. Las conferencias de audio y video en tiempo real y la transmisión de contenido en vivo tienen especial interés en investigación, ya que la prospectiva tecnológica estima que el tráfico de video supere a cualquier otro tipo de tráfico de datos en el futuro cercano. La transmisión en vivo se refiere a aplicaciones en las que flujos de audio/vídeo de una fuente se han de entregar a un conjunto de destinos en lugares geográficos diferentes mientras se mantiene baja la latencia de entrega del flujo (como por ejemplo la cobertura de eventos en vivo). Las plataformas de conferencia en tiempo real son plataformas de aplicación que implementan comunicaciones de audio/video en tiempo real entre muchos participantes. Ambas categorías presentan una alta sensibilidad tanto al estado de la red (latencia, jitter, pérdida de paquetes, velocidad de bits) como a los perfiles de carga de la infraestructura de procesamiento de flujo (latencia y jitter introducidos durante el procesamiento en la nube de paquetes de datos multimedia). Esta tesis trata de mejorar el procesamiento de datos multimedia en tiempo real tanto en los parámetros de nivel de red como en las optimizaciones en la nube.