bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibody induction and immune response in nasal cavity by third dose of SARS-CoV-2 mRNA vaccination
Authors: Ishizaka Aya , Koga Michiko , Mizutani Taketoshi , Uraki Ryuta , Yamayoshi Seiya , Iwatsuki-Horimoto Kiyoko , Yamamoto Shinya , Imai Masaki , Tsutsumi Takeya , Suzuki Yutaka , Kawaoka Yoshihiro , Yotsuyanagi Hiroshi 
Published: July 2023

Abstract
Background
The mucosa serves as the first defence against pathogens and facilitates the surveillance and elimination of symbiotic bacteria by mucosal immunity. Recently, the mRNA vaccine against SARS-CoV-2 has been demonstrated to induce secretory antibodies in the oral and nasal cavities in addition to a systemic immune response. However, the mechanism of induced immune stimulation effect on mucosal immunity and commensal bacteria profile remains unclear.

Methods
Here, we longitudinally analysed the changing nasal microbiota and both systemic and nasal immune response upon SARS-CoV-2 mRNA vaccination, and evaluated how mRNA vaccination influenced nasal microbiota in 18 healthy participants who had received the third BNT162b.

Results
The nasal S-RBD IgG level correlated significantly with plasma IgG levels until 1 month and the levels were sustained for 3 months post-vaccination. In contrast, nasal S-RBD IgA induction peaked at 1 month, albeit slightly, and correlated only with plasma IgA, but the induction level decreased markedly at 3 months post-vaccination. 16 S rRNA sequencing of the nasal microbiota post-vaccination revealed not an overall change, but a decrease in certain opportunistic bacteria, mainly Fusobacterium. The decrease in these bacteria was more pronounced in those who exhibited nasal S-RBD IgA induction, and those with higher S-RBD IgA induction had lower relative amounts of potentially pathogenic bacteria such as Pseudomonas pre-vaccination. In addition, plasma and mucosal S-RBD IgG levels correlated with decreased commensal pathogens such as Finegoldia.

Conclusions
These findings suggest that the third dose of SARS-CoV-2 mRNA vaccination induced S-RBD antibodies in the nasal mucosa and may have stimulated mucosal immunity against opportunistic bacterial pathogens. This effect, albeit probably secondary, may be considered one of the benefits of mRNA vaccination. Furthermore, our data suggest that a cooperative function of mucosal and systemic immunity in the reduction of bacteria and provides a better understanding of the symbiotic relationship between the host and bacteria in the nasal mucosa.
Metabolic syndrome criteria and severity and carbon dioxide (CO2) emissions in an adult population
Authors: García Silvia , Pastor Rosario , Monserrat-Mesquida Margalida , Álvarez-Álvarez Laura , Rubín-García María , Martínez-González Miguel Ángel , Salas-Salvadó Jordi , Corella Dolores , Goday Albert , Martínez J. Alfredo , Alonso-Gómez Ángel M. , Wärnberg Julia , Vioque Jesús , Romaguera Dora , Lopez-Miranda José , Estruch Ramon , Tinahones Francisco J. , Lapetra José , Serra-Majem Lluís , Riquelme-Gallego Blanca , Pintó Xavier , Gaforio José J. , Matía Pilar , Vidal Josep , Vázquez Clotilde , Daimiel Lidia , Ros Emilio , Sayón-Orea Carmen , Guillem-Saiz Patricia , Valle-Hita Cristina , Cabanes Robert , Abete Itziar , Goicolea-Güemez Leire , Gómez-Gracia Enrique , Tercero-Maciá Cristina , Colom Antoni , García-Ríos Antonio , Castro-Barquero Sara , Fernández-García José C. , Santos-Lozano José Manuel , Cenoz Juan Carlos , Barragán Rocío , Khoury Nadine , Castañer Olga , Zulet María Ángeles , Vaquero-Luna Jessica , Bes-Rastrollo Maira , de las Heras-Delgado Sara , Ciurana Ramon , Martín-Sánchez Vicente , Tur Josep A. , Bouzas Cristina 
Published: July 2023

Abstract
Background
Metabolic syndrome (MetS) has become a growing risk factor of some non-communicable diseases. Increase of greenhouse gas emissions affects the planet.

Aims
To assess the association between MetS severity and amount of carbon dioxide (CO2) emitted in an adult population.

Design
Cross-sectional study (n = 6646; 55-76-year-old-men; 60-75-year-old-women with MetS).

Methods
Dietary habits were assessed using a pre-validated semi quantitative 143-item food frequency questionnaire. The amount of CO2 emitted due to the production of food consumed by person and day was calculated using a European database, and the severity of the MetS was calculated with the MetS Severity Score.

Results
Higher glycaemia levels were found in people with higher CO2 emissions. The risk of having high severe MetS was related to high CO2 emissions.

Conclusions
Low CO2 emissions diet would help to reduce MetS severity. Advantages for both health and the environment were found following a more sustainable diet.

Trial registration
ISRCTN, ISRCTN89898870. Registered 05 September 2013.
Diaphragm and abdominal organ motion during radiotherapy: a comprehensive multicenter study in 189 children
Authors: Meijer Karin M. , van Dijk Irma W. E. M. , Frank Marije , van den Hoek Arnout D. , Balgobind Brian V. , Janssens Geert O. , Wendling Markus , Maduro John H. , Bryce-Atkinson Abigail , Loginova Anna , Bel Arjan 
Published: July 2023

AbstractBackgroundFor accurate thoracic and abdominal radiotherapy, inter- and intrafractional geometrical uncertainties need to be considered to enable accurate margin sizes. We aim to quantify interfractional diaphragm and abdominal organ position variations, and intrafractional diaphragm motion in a large multicenter cohort of pediatric cancer patients (< 18 years). We investigated the correlation of interfractional position variations and intrafractional motion with age, and with general anesthesia (GA).MethodsIn 189 children (mean age 8.1; range 0.4–17.9 years) from six institutes, interfractional position variation of both hemidiaphragms, spleen, liver, left and right kidneys was quantified using a two-step registration. CBCTs were registered to the reference CT relative to the bony anatomy, followed by organ registration. We calculated the group mean, systematic and random errors (standard deviations Σ and σ, respectively) in cranial-caudal (CC), left-right and anterior-posterior directions. Intrafractional right hemidiaphragm motion was quantified using CBCTs on which the breathing amplitude, defined as the difference between end-inspiration and end-expiration peaks, was assessed (N = 79). We investigated correlations with age (Spearman’s ρ), and differences in motion between patients treated with and without GA (N = 75; all < 5.5 years).ResultsInterfractional group means were largest in CC direction and varied widely between patients, with largest variations in the right hemidiaphragm (range -13.0–17.5 mm). Interfractional group mean of the left kidney showed a borderline significant correlation with age (p = 0.047; ρ = 0.17). Intrafractional right hemidiaphragm motion in patients ≥ 5.5 years (mean 10.3 mm) was significantly larger compared to patients < 5.5 years treated without GA (mean 8.3 mm) (p = 0.02), with smaller Σ and σ values. We found a significant correlation between breathing amplitude and age (p < 0.001; ρ = 0.43). Interfractional right hemidiaphragm position variations were significantly smaller in patients < 5.5 years treated with GA than without GA (p = 0.004), but intrafractional motion showed no significant difference.ConclusionIn this large multicenter cohort of children undergoing thoracic and abdominal radiotherapy, we found that interfractional position variation does not depend on age, but the use of GA in patients < 5.5 years showed smaller systematic and random errors. Furthermore, our results showed that breathing amplitude increases with age. Moreover, variations between patients advocate the need for a patient-specific margin approach.
Deriving emission factors for mangrove blue carbon ecosystem in Indonesia
Authors: Murdiyarso Daniel , Krisnawati Haruni , Adinugroho Wahyu C. , Sasmito Sigit D. 
Published: July 2023

Abstract
Background
Using ‘higher-tier’ emission factors in National Greenhouse Gas Inventories is essential to improve quality and accuracy when reporting carbon emissions and removals. Here we systematically reviewed 736 data across 249 sites (published 2003–2020) to derive emission factors associated with land-use change in Indonesian mangroves blue carbon ecosystems.

Results
Four management regimes—aquaculture, degraded mangrove, regenerated mangrove and undisturbed mangrove—gave mean total ecosystem carbon stocks of 579, 717, 890, and 1061 Mg C ha−1 respectively. The largest biomass carbon stocks were found in undisturbed mangrove; followed by regenerated mangrove, degraded mangrove, and aquaculture. Top 100-cm soil carbon stocks were similar across regimes, ranging between 216 and 296 Mg C ha−1. Carbon stocks between 0 and 300 cm varied significantly; the highest values were found in undisturbed mangrove (916 Mg C ha−1), followed by regenerated mangrove (803 Mg C ha−1), degraded mangrove 666 Mg C ha−1), and aquaculture (562 Mg C ha−1).

Conclusions
Using deep layer (e.g., 300 cm) soil carbon stocks would compensate for the underestimation of surface soil carbon removed from areas where aquaculture is widely practised. From a project perspective, deep layer data could secure permanence or buffer potential leakages. From a national GHG accounting perspective, it also provides a safeguard in the MRV system.
Hypomyelination caused by a novel homozygous pathogenic variant in FOLR1: complete clinical and radiological recovery with oral folinic acid therapy and review of the literature
Authors: Potic Ana , Perrier Stefanie , Radovic Tijana , Gavrilovic Svetlana , Ostojic Jelena , Tran Luan T. , Thiffault Isabelle , Pastinen Tomi , Schiffmann Raphael , Bernard Geneviève 
Published: July 2023

Abstract
Background
Neurodegeneration due to cerebral folate transport deficiency is a rare autosomal recessive disorder caused by biallelic pathogenic variants in FOLR1. Onset typically occurs in late infancy and is characterized by psychomotor regression, epilepsy, and a hypomyelinating leukodystrophy on magnetic resonance imaging. If left untreated, progressive neurodegeneration occurs. However, early treatment with folinic acid has been shown to stabilize or reverse neurological features. Approximately thirty patients have been described worldwide. Here, we report the first two cases with genetically proven cerebral folate transport deficiency from South-Eastern Europe, describe the effect of oral folinic acid therapy on clinical and neuroradiological features and review the literature.

Results
Two siblings presented in childhood with clinical and radiological findings consistent with a hypomyelinating leukodystrophy. Exome sequencing revealed a novel homozygous pathogenic variant in FOLR1 (c.465_466delinsTG; p.W156G), confirming the diagnosis of neurodegeneration due to cerebral folate transport deficiency. Folinic acid treatment was promptly initiated in both patients. The younger sibling was treated early in disease course at 2 years of age, and demonstrated complete recovery in clinical and MRI features. The older sibling, who was 8 years of age at the time of diagnosis and treatment, demonstrated partial but substantial improvements.

Conclusion
We present the first account in the literature that early treatment initiation with oral folinic acid alone can result in complete neurological recovery of both clinical and radiological abnormalities in neurodegeneration due to cerebral folate deficiency. Moreover, through the report of these patients along with review of the literature, we provide information about the natural history of the disease with comparison of treatment effects at different stages of disease progression. This report also reinforces the importance of universal access to genetic testing to ensure prompt diagnoses for treatable disorders.
Stability selection enhances feature selection and enables accurate prediction of gestational age using only five DNA methylation sites
Authors: Haftorn Kristine L. , Romanowska Julia , Lee Yunsung , Page Christian M. , Magnus Per M. , Håberg Siri E. , Bohlin Jon , Jugessur Astanand , Denault William R. P. 
Published: July 2023

AbstractBackgroundDNA methylation (DNAm) is robustly associated with chronological age in children and adults, and gestational age (GA) in newborns. This property has enabled the development of several epigenetic clocks that can accurately predict chronological age and GA. However, the lack of overlap in predictive CpGs across different epigenetic clocks remains elusive. Our main aim was therefore to identify and characterize CpGs that are stably predictive of GA.ResultsWe applied a statistical approach called ‘stability selection’ to DNAm data from 2138 newborns in the Norwegian Mother, Father, and Child Cohort study. Stability selection combines subsampling with variable selection to restrict the number of false discoveries in the set of selected variables. Twenty-four CpGs were identified as being stably predictive of GA. Intriguingly, only up to 10% of the CpGs in previous GA clocks were found to be stably selected. Based on these results, we used generalized additive model regression to develop a new GA clock consisting of only five CpGs, which showed a similar predictive performance as previous GA clocks (R2 = 0.674, median absolute deviation = 4.4 days). These CpGs were in or near genes and regulatory regions involved in immune responses, metabolism, and developmental processes. Furthermore, accounting for nonlinear associations improved prediction performance in preterm newborns.ConclusionWe present a methodological framework for feature selection that is broadly applicable to any trait that can be predicted from DNAm data. We demonstrate its utility by identifying CpGs that are highly predictive of GA and present a new and highly performant GA clock based on only five CpGs that is more amenable to a clinical setting.
Neoadjuvant therapy for sarcomatoid carcinoma of the pancreas: a case report and review of the literature
Authors: Qiu Hui , Hao Chun-Yi 
Published: July 2023

Abstract
Background
Sarcomatoid carcinoma of the pancreas (SCP) is a rare type of malignant pancreatic neoplasm, and its prognosis is even worse than that of conventional pancreatic ductal adenocarcinoma (PDAC). Currently, there is no standard regimen for treating SCP, and the impact of systemic therapy on the survival of patients with SCP has not been well defined.

Case presentation
Herein, we report a 38-year-old Asian man diagnosed of local unresectable SCP with supraclavicular lymph node metastasis, radical excision after camrelizumab and anlotinib therapy, which resulted in a remarkable reduction in the size of primary tumor and complete remission of the metastatic lymph node.

Conclusions
This is the first report of the use of immunotherapy and anti-angiogenesis therapy in a patient with SCP, which provides optimistic data to support the synergistic effect.
Single-base station hybrid positioning algorithm based on LOS identification
Authors: Gao Yang , Jiao Zihan , Wu Qiang , Dou Xiaoyuan , Fan Jiancun 
Published: July 2023

AbstractIn the complex multipath propagation environment, whether there is a line of sight (LOS) path will directly affect the positioning accuracy. Therefore, this paper proposes a single base station hybrid positioning algorithm based on LOS identification. In the algorithm, we first construct multiple features based on channel state information with LOS and without LOS in statistical distribution and use these features and gradient boosting decision tree to determine whether there is a LOS path in the environment. Then for the environment with a LOS path, we proposed a positioning method based on the estimation of signal parameters via rotation invariant technology algorithm which can be used to jointly estimate the angle of arrival and time delay of the LOS path for positioning, while for the environment without LOS path, we propose a positioning method based on adaptive genetic algorithm. Finally, a single base station hybrid positioning algorithm based on LOS identification and the corresponding positioning methods. Simulation results show that the proposed hybrid positioning algorithm can achieve high-precision positioning in the complex multipath propagation environment.
What makes people grow? Love and hope
Authors: Bogin Barry 
Published: July 2023

AbstractBackgroundHope and love are popular themes of literature and art in many human societies. The human physiology of love and hope is less well understood. This review presents evidence that the lack of love and/or hope delays growth disturbs development and maturation and even kills.Main bodyLove and hope intersect in promoting healthy human development. Love provides a sense of security and attachment, which are necessary for healthy physical, cognitive, and emotional development. Hope provides a sense of optimism and resilience in the face of adversity. Loving relationships can foster a sense of hope in individuals and in society by providing support systems during difficult times. Similarly, having a sense of hope can make it easier to form loving relationships by providing individuals with the confidence to connect with others. Hope and love are the fundamental basis of human biocultural reproduction, which is the human style of cooperation in the production, feeding, and care of offspring. Examples are given of the association between human growth in height with love and hope, including (1) the global “Long Depression” of 1873–1896, (2) “hospitalism” and the abuse/neglect of infants and children, (3) adoption, (4) international migration, (5) colonial conquest, and (6) social, economic, and political change in Japan between 1970 and 1990.ConclusionOverall, this review suggests that love and hope are both critical factors in promoting healthy human development and that they intersect in complex ways to support emotional well-being.
Hyperbaric oxygen treatment increases intestinal stem cell proliferation through the mTORC1/S6K1 signaling pathway in Mus musculus
Authors: Casanova-Maldonado Ignacio , Arancibia David , Lois Pablo , Peña-Villalobos Isaac , Palma Verónica 
Published: July 2023

Abstract
Background
Hyperbaric oxygen treatment (HBOT) has been reported to modulate the proliferation of neural and mesenchymal stem cell populations, but the molecular mechanisms underlying these effects are not completely understood. In this study, we aimed to assess HBOT somatic stem cell modulation by evaluating the role of the mTOR complex 1 (mTORC1), a key regulator of cell metabolism whose activity is modified depending on oxygen levels, as a potential mediator of HBOT in murine intestinal stem cells (ISCs).

Results
We discovered that acute HBOT synchronously increases the proliferation of ISCs without affecting the animal’s oxidative metabolism through activation of the mTORC1/S6K1 axis. mTORC1 inhibition by rapamycin administration for 20 days also increases ISCs proliferation, generating a paradoxical response in mice intestines, and has been proposed to mimic a partial starvation state. Interestingly, the combination of HBOT and rapamycin does not have a synergic effect, possibly due to their differential impact on the mTORC1/S6K1 axis.

Conclusions
HBOT can induce an increase in ISCs proliferation along with other cell populations within the crypt through mTORC1/S6K1 modulation without altering the oxidative metabolism of the animal’s small intestine. These results shed light on the molecular mechanisms underlying HBOT therapeutic action, laying the groundwork for future studies.
Perceptions of dietary intake amongst Black, Asian and other minority ethnic groups in high-income countries: a systematic review of qualitative literature
Authors: Ojo Abimbola S. , Nnyanzi Lawrence A. , Giles Emma L. , Ells Louisa J. , Awolaran Olusegun , Okeke Sylvester R. , Afaya Agani , Bolarinwa Obasanjo Afolabi 
Published: July 2023

Abstract
Background
Minority ethnic groups are a fast-growing population in many high-income countries, partly due to the increasing population of immigrants and second-generation migrants. The dietary practices of some of these minority ethnic groups might make them to be disproportionately affected by obesity and increase their risks of developing non-communicable diseases. Population-specific interventions and strategies are vital to addressing poor nutritional practices among this population. Thus, this study systematically reviewed the perceptions of dietary intake amongst Black, Asian and other minority ethnic groups in high-income countries.

Methods
This systematic review was conducted in line with the guidelines of the Joanna Briggs Institute (JBI) methodology for systematic reviews, using a meta-aggregative design. This systematic review identified and synthesised qualitative literature on the perceptions of dietary intake amongst BlackAsian and other minority ethnic groups in high-income countries. An extensive and comprehensive database search was conducted between January 2000 – May 2022 and included twenty (20) studies that met the eligibility criteria from six countries. The included studies were assessed for quality using the JBI qualitative assessment and review instrument. The JBI data extraction tools were used to retrieve relevant data from included articles, and the data were thematically analysed.

Results
We identified eight major themes across this database: (1) “Social and Cultural Factors,” (2) “Availability and Accessibility,” (3) “Family and Community Influences,” (4) “Food Preferences”, (5) “Home Country Food Versus Host Country Food” (6) “Dietary Acculturation” (7) “Health and Healthy Eating” (8) “Perception of Nutritional Information.”

Conclusion
Overall, Black, Asian, and other minority ethnic groups individuals were found to be aware of the effects of unhealthy eating on their health, and some of them have nutritional knowledge, but social and cultural factors, including structural factors, were deterrents to their healthy eating behaviours. An important finding from this review is that some participants believed that nutritional information, based on bio-medical science, was intended for only White population groups and that it was antagonistic to their cultural and community well-being.