bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ablation of Argonaute 2 in Schwann cells accelerates the progression of diabetic peripheral neuropathy
Authors: Fan Baoyan , Chopp Michael , Zhang Yi , Wang Xinli , Kemper Amy , Zhang Zheng Gang , Liu Xian Shuang 
Published: May 2023

AbstractSchwann cells (SCs) form myelin and provide metabolic support for axons, and are essential for normal nerve function. Identification of key molecules specific to SCs and nerve fibers may provide new therapeutic targets for diabetic peripheral neuropathy (DPN). Argonaute2 (Ago2) is a key molecular player that mediates the activity of miRNA‐guided mRNA cleavage and miRNA stability. Our study found that Ago2 knockout (Ago2‐KO) in proteolipid protein (PLP) lineage SCs in mice resulted in a significant reduction of nerve conduction velocities and impairments of thermal and mechanical sensitivities. Histopathological data revealed that Ago2‐KO significantly induced demyelination and neurodegeneration. When DPN was induced in both wild‐type and Ago2‐KO mice, Ago2‐KO mice exhibited further decreased myelin thickness and exacerbated neurological outcomes compared with wild‐type mice. Deep sequencing analysis of Ago2 immunoprecipitated complexes showed that deregulated miR‐206 in Ago2‐KO mice is highly related to mitochondrial function. In vitro data showed that knockdown of miR‐200 induced mitochondrial dysfunction and apoptosis in SCs. Together, our data suggest that Ago2 in SCs is essential to maintain peripheral nerve function while ablation of Ago2 in SCs exacerbates SC dysfunction and neuronal degeneration in DPN. These findings provide new insight into the molecular mechanisms of DPN.
Adaptation of a Brazilian university hospital to clinical treatment of ectopic pregnancy: Lessons learned over 17 years
Authors: Tavares Bárbara V. G. , Delfino Letícia S. , Ignarro Isabella S. , Baccaro Luiz F. 
Published: May 2023

AbstractObjectiveTo describe and compare the annual success rates of medical treatment in the analyzed period and to evaluate the associated factors.MethodsRetrospective study with 158 women with tubal pregnancy followed up over 17 years. Statistical analysis was performed using the Cochran‐Armitage test, the χ2 test, Mann–Whitney test, and multiple logistic regression.ResultsThe success rate was 47.4%. There was a trend of significant change in the success rate of clinical treatment over time (Z = 2.01, P = 0.044); it was associated to undergoing treatment between 2012 and 2017 (P = 0.028), the absence of abdominal pain (P = 0.020), receiving a higher dose of methotrexate (P < 0.001), and less time hospitalized (P < 0.001). In the final statistical model, we observed that receiving a higher dose of methotrexate (P = 0.025, odds ratio [OR] 1.03, 95% confidence interval [CI] 1.00–1.06), having a low serum β‐HCG concentration before treatment (P = 0.003, OR 0.87, 95% CI 0.79–0.95), and not having abdominal pain (P = 0.004, OR 4.26, 95% CI 1.61–11.28) were factors associated with a higher chance of successful clinical treatment for tubal pregnancy.ConclusionA greater chance of success was observed among women undergoing clinical treatment from 2012 onwards, who used higher doses of methotrexate, were asymptomatic at admission, and had low concentrations of β‐hCG.
Predictors of maternal satisfaction with labor induction: A prospective observational cohort study
Authors: Ramlee Nurbayani , Azhary Jerilee Mariam Khong , Hamdan Mukhri , Saaid Rahmah , Gan Farah , Tan Peng Chiong 
Published: May 2023

AbstractObjectiveTo identify independent predictors of maternal satisfaction with labor induction.MethodIn this prospective observational cohort study, 769 women prior to their labor induction had sleep and psychological well‐being assessed using Pittsburgh Sleep Quality Index and Depression, Anxiety and Stress Scales. Women were asked about the adequacy of labor induction information provided and their involvement and time pressure felt in the decision‐making for their labor induction. Maternal characteristics, induction and intrapartum care measures, and labor and neonatal outcomes were also assessed. Prior to discharge, women rated their satisfaction with their birth experience.ResultsA total of 34 variables were considered for bivariate analysis, with 15 found to have P < 0.05. Following adjusted analysis, 10 independent predictors of maternal satisfaction were identified: maternal education, previous cesarean delivery, maternal involvement, information provided, and decision‐making time pressure regarding labor induction, amniotomy, induction to delivery interval, mode of delivery, postpartum hemorrhage, and neonatal admission. Maternal satisfaction was not associated with sleep, depression, anxiety, or stress.ConclusionThe identification of independent predictors of maternal satisfaction allows for patient selection, targeting of specific preinduction and intrapartum care, and focus on induction methods that can reduce induction to delivery interval, cesarean birth, and delivery blood loss to maximize women's satisfaction with labor induction.
Pregnancy after laparoscopic surgery for endometriosis: How long should we wait? A retrospective study involving a long‐term follow up at a university endometriosis center
Authors: Tahmasbi Rad Morva , Akpinar‐Isci Dilara , Nobs Tatjana , Gasimli Khayal , Becker Sven 
Published: May 2023

AbstractObjectiveTo find the factors that influence the time until pregnancy after laparoscopic intervention and to evaluate the proper time to start assisted reproductive therapy (ART).MethodsThis is a retrospective analysis that includes a prospective evaluation of patients with infertility and with a diagnosis of endometriosis. A subgroup of patients who desired to be pregnant after laparoscopic intervention (102 patients) was followed up for 36–197 months after the surgery.ResultsIn this study, 71 (69.9%) and 60 (58.8%) of the patients achieved pregnancy and live birth, respectively. In the group of patients who became pregnant, the duration of infertility was significantly lower (2.7 ± 2.1 years vs. 4.7 ± 3.2 years). The median time until pregnancy after laparoscopic intervention was 8 months (the average was 10 months). After 38 months, no pregnancy occurred.ConclusionIn the group of patients with endometriosis and infertility, the first 12 months were the optimal time for pregnancy. For women with revised American Society for Reproductive Medicine classification of endometriosis (rASRM) stages I and II, spontaneous pregnancy can probably be delayed for up to 24 months, but in patients with rASRM stages III and IV, ART may be considered after 12 months. The gynecologist must be careful about the timing of further interventions after the operation.
The impact of extending the second stage of labor on repeat cesarean section and maternal and neonatal outcome
Authors: Lauterbach Roy , Justman Naphtali , Ginsberg Yuval , Siegler Yoav , Bachar Gal , Vitner Dana , Ben‐David Chen , Zipori Yaniv , Beloosesky Ron , Weiner Zeev , Khatib Nizar 
Published: May 2023

AbstractObjectiveTo evaluate the effects of extending the second stage of labor in women attempting a trial of labor after a cesarean section (TOLAC).MethodA retrospective cohort study comparing maternal and neonatal outcomes following TOLAC over two periods: period I whose prolonged second stage was considered 2 h, and period II whose prolonged second stage was considered 3 h. The primary outcome was repeat cesarean delivery (CD) rate.ResultsIncidence of repeat CD was significantly lower in period II (18.1% vs 29.7%, P < 0.001). Incidence of uterine rupture was significantly higher in period II (P < 0.001). Instrumental delivery rates were significantly higher in period II (26.2% vs 15.6%, odds ratio [OR] 1.67, 95% CI 1.21–3.56, P < 0.001). Rates of third‐ and fourth‐degree perineal lacerations, chorioamnionitis, and length of hospital stay were similar between groups. Incidence of fetal acidemia was significantly higher in period II (1.5% vs 0.7%, OR 2.14, 95% CI 1.32–5.63, P < 0.001), and incidence of neonatal intensive care unit (NICU) admission was significantly higher (2.5% vs 1.6%, P = 0.004).ConclusionExtension of the second stage of labor is associated with a decrease in repeat CD rate with a concomitant increase in instrumental delivery rates, uterine rupture, fetal acidemia, and NICU admissions. These findings may warrant further consideration of allowing a prolonged second stage in patients attempting TOLAC.
An in‐depth analysis of maternal mortality in a quaternary university hospital in Southern Thailand: A 16‐year experience
Authors: Peeyananjarassri Krantarat , Ratipann Ninizna , Suwanrath Chitkasaem , Pinjaroen Sutham , Chainarong Natthicha , Choobun Thanapan 
Published: May 2023

AbstractObjectiveTo perform an in‐depth analysis and evaluate the causes of maternal deaths in a university hospital in Southern Thailand.MethodsA retrospective descriptive study was conducted. Medical records of all women who died during pregnancy or within 42 days of termination of pregnancy at Songklanagarind Hospital from January 2006 to December 2021 were reviewed.ResultsThere were 51 910 live births during the 16‐year period and 68 maternal deaths (31 of direct cause, 36 of indirect cause, and one of unknown cause). The maternal mortality ratio was 131 per 100 000 live births. There was a decreasing trend in the maternal mortality ratio, especially during the past 4 years. The leading causes of death were cardiac disease (n = 17), hypertensive disorders in pregnancy (n = 9), and postpartum hemorrhage (n = 7). The most common indirect causes of maternal death were cardiac disease, hematologic disease, and dengue hemorrhagic fever. There was a decreasing trend in indirect to direct deaths ratios during three study periods. Ratios in 2006 to 2010, 2011 to 2015, and 2016 to 2021 were 1.6, 1.4, and 0.5, respectively.ConclusionsThe three leading causes of death were cardiac disease, hypertensive disorders in pregnancy, and postpartum hemorrhage. Improvements in the quality of obstetric care at all hospital levels are required.
Endoscopic optical coherence tomography angiography using an externally driving catheter
Authors: Gao Lei , Wu Zhengjie , Chen Zhuoquan , Kong Ruiming , Song Yuting , Ma Teng , Dai Cuixia 
Published: May 2023

AbstractEndoscopic optical coherence tomography (OCT) is an imaging modality that enables cross‐sectional subsurface imaging of tubular organs and cavities. Recently, endoscopic OCT angiography (OCTA) was successfully achieved in distal scanning systems using an internal‐motor‐driving catheter. In conventional OCT systems using externally driving catheters, the mechanical instability in the proximal actuation causes difficulties for differentiating capillaries in tissues. In this study, OCTA in an endoscopic OCT system using an external‐motor‐driving catheter was proposed. Blood vessels were visualized by using a high‐stability inter‐A‐scan scheme and the spatiotemporal singular value decomposition algorithm. It is not limited by nonuniform rotation distortion caused by the catheter and physiological motion artifacts. Results show that microvasculature in a custom‐made microfluidic phantom and the submucosal capillaries in the mouse rectum are successfully visualized. Furthermore, OCTA using a catheter with a small size (outer diameter less than 1 mm) makes it possible for early diagnosis of narrow lumens, such as pancreatic and bile duct cancers.
Novice school counselors’ countertransference management on emotional exhaustion: The role of daily emotional processes
Authors: Hong Soeun , Lee Taerim , Ko Hyeyun , Kang Jieun , Jang Gi‐Eun , Lee Sang Min 
Published: May 2023

AbstractThe present study aimed to examine the role of daily emotional processes in the relationship between countertransference (CT) management and emotional well‐being in novice school counselors. We collected data for 7 consecutive days from 305 novice school counselors in South Korea. Multilevel mediation analyses revealed that novice school counselors with low CT management ability were less likely to be aware of their emotions, increasing their daily emotional exhaustion. In contrast, novice school counselors with a high level of CT management ability were more likely to engage in daily decentering and, in turn, were less likely to experience daily emotional exhaustion. Although emotional inhibition (lack of emotional awareness) significantly exacerbated emotional exhaustion over time, decentering was not related to emotional exhaustion over time. The practical and theoretical implications of this study are discussed.
A meta‐analysis of supervisee attachment style and the supervisory relationship
Authors: McKibben W. Bradley , Lenz A. Stephen , Sheperis Donna 
Published: May 2023

AbstractAttachment theory has been used to explain supervisee/supervisor bonding in clinical supervision, but research on these constructs has been mixed. To test the extent to which supervisee attachment strategies relate to the supervisory relationship, the authors conducted a meta‐analysis of 19 published research studies on supervisee attachment from 1998 to 2022. Supervisee attachment strategies produced a small but statistically significant effect on the supervisory relationship (R2 = 0.052). We discuss this finding in the light of previous mixed results, including that the supervisory relationship may not be characterized by a full‐blown attachment bond. We recommend additional areas of research on attachment in supervision and highlight additional supervisory models to conceptualize and study the supervisory relationship.
Counseling interventions for victims of intimate partner violence: A systematic review
Authors: Craven Laura C. , Fields Alexander M. , Carlson Ryan G. , Combs Elizabeth M. , Howe Emily S. 
Published: May 2023

AbstractIntimate partner violence is a serious public health problem with significant implications for the mental health and well‐being of victims. Therefore, we sought to critically review research on counseling interventions for victims to inform counselors’ work and identify areas of need for future research. We used the preferred reporting items for systematic reviews and meta‐analyses protocols to identify articles from 2000 to 2022 that investigated the impact of counseling interventions on the mental health and safety of victims. Four themes emerged: (1) participant demographics, (2) treatment modality, (3) interventions and study outcomes, and (4) study limitations. Study findings are synthesized to help counselors identify effective interventions for victims based on mental health concerns and violence type. Many of the studies were limited by high attrition rates and few researchers implemented randomized controlled trials. Further research is needed on interventions for LGBTQ victims and victims who present with comorbid mental health concerns.
IGMPlot: A program to identify, characterize, and quantify molecular interactions
Authors: Lefebvre C. , Klein J. , Khartabil H. , Boisson J.‐C. , Hénon E. 
Published: May 2023

AbstractWe describe the development and features of a program called IGMPlot, which is based on the independent gradient model (IGM) and its local descriptor . The IGM approach analyzes the gradient of the electron density (ED) in a molecular system to identify regions of space where chemical interactions take place. IGMPlot is intended for use by both experimental scientists and theoretical chemists. It is standalone software written in C++, with versions available for multiple platforms. Some key features are: probing and quantifying interactions between two given molecular fragments, determining bond strength (IBSI), estimating the atomic contributions to an intermolecular interaction and preparing data to build 2D and 3D representations of interaction regions. The software has been updated to include new features: critical point analysis of the ED, assessing ED asymmetry of a given bond (PDA) and a new descriptor called designed to enhance the IGM‐ analysis. The program can be found at:http://igmplot.univ-reims.fr.
BinderSpace: A package for sequence space analyses for datasets of affinity‐selected oligonucleotides and peptide‐based molecules
Authors: Kelich Payam , Zhao Huanhuan , Orona Jose R. , Vuković Lela 
Published: May 2023

AbstractDiscovery of target‐binding molecules, such as aptamers and peptides, is usually performed with the use of high‐throughput experimental screening methods. These methods typically generate large datasets of sequences of target‐binding molecules, which can be enriched with high affinity binders. However, the identification of the highest affinity binders from these large datasets often requires additional low‐throughput experiments or other approaches. Bioinformatics‐based analyses could be helpful to better understand these large datasets and identify the parts of the sequence space enriched with high affinity binders. BinderSpace is an open‐source Python package that performs motif analysis, sequence space visualization, clustering analyses, and sequence extraction from clusters of interest. The motif analysis, resulting in text‐based and visual output of motifs, can also provide heat maps of previously measured user‐defined functional properties for all the motif‐containing molecules. Users can also run principal component analysis (PCA) and t‐distributed stochastic neighbor embedding (t‐SNE) analyses on whole datasets and on motif‐related subsets of the data. Functionally important sequences can also be highlighted in the resulting PCA and t‐SNE maps. If points (sequences) in two‐dimensional maps in PCA or t‐SNE space form clusters, users can perform clustering analyses on their data, and extract sequences from clusters of interest. We demonstrate the use of BinderSpace on a dataset of oligonucleotides binding to single‐wall carbon nanotubes in the presence and absence of a bioanalyte, and on a dataset of cyclic peptidomimetics binding to bovine carbonic anhydrase protein. BinderSpace is openly accessible to the public via the GitHub website: https://github.com/vukoviclab/BinderSpace.
Off track or on? Associations of positive and negative life events with the continuation versus cessation of repetitive adolescent nonsuicidal self‐injury
Authors: Claréus Benjamin , Daukantaité Daiva 
Published: May 2023

AbstractObjectiveThis study examined how patterns of repetitive (≥5 instances) nonsuicidal self‐injury (NSSI) associate with measures of resilience and life events retrospectively reported to have occurred within the last year, 1 to <5 years ago, and 5 to <10 years ago.MethodLife events reported by 557 young adults (mean [SD] age 25.3 [0.68]; 59.2% women) were classified as positive, negative, or profoundly negative based on their relationship to participants' mental health and well‐being. We subsequently examined how these categories, together with resilience, were cross‐sectionally associated with reporting no NSSI, and the (full/partial) cessation/continuation of repetitive NSSI from adolescence to young adulthood.ResultsRepetitive NSSI in adolescence was associated with (profoundly) negative life events. Relative to cessation, NSSI continuation was significantly associated with more kinds of negative life events (odds ratio [OR] = 1.79) and fewer kinds of positive life events 1 to <5 years ago (OR = 0.65) and tended to be associated with lower resilience (b = −0.63, p = 0.056). Neither life events nor resilience significantly differentiated individuals reporting full or partial cessation.ConclusionResilience appears important for the cessation of repetitive NSSI, but contextual factors must still be considered. Assessing positive life events in future studies holds promise.
Cachexia causes time‐dependent activation of the inflammasome in the liver
Authors: das Neves Rodrigo Xavier , Yamashita Alex S. , Riccardi Daniela M.R. , Köhn‐Gaone Julia , Camargo Rodolfo G. , Neto Nelson I. , Caetano Daniela , Gomes Silvio P. , Santos Felipe H. , Lima Joanna D.C.C. , Batista Miguel L. , Rosa‐Neto José Cesar , Martins De Alcântara Paulo Sérgio , Maximiano Linda F. , Otoch José P. , Trinchieri Giorgio , Tirnitz‐Parker Janina E.E. , Seelaender Marília 
Published: May 2023

AbstractBackgroundCachexia is a wasting syndrome associated with systemic inflammation and metabolic disruption. Detection of the early signs of the disease may contribute to the effective attenuation of associated symptoms. Despite playing a central role in the control of metabolism and inflammation, the liver has received little attention in cachexia. We previously described relevant disruption of metabolic pathways in the organ in an animal model of cachexia, and herein, we adopt the same model to investigate temporal onset of inflammation in the liver. The aim was thus to study inflammation in rodent liver in the well‐characterized cachexia model of Walker 256 carcinosarcoma and, in addition, to describe inflammatory alterations in the liver of one cachectic colon cancer patient, as compared to one control and one weight‐stable cancer patient.MethodsColon cancer patients (one weight stable [WSC] and one cachectic [CC]) and one patient undergoing surgery for cholelithiasis (control, n = 1) were enrolled in the study, after obtainment of fully informed consent. Eight‐week‐old male rats were subcutaneously inoculated with a Walker 256 carcinosarcoma cell suspension (2 × 107 cells in 1.0 mL; tumour‐bearing [T]; or phosphate‐buffered saline—controls [C]). The liver was excised on Days 0 (n = 5), 7 (n = 5) and 14 (n = 5) after tumour cell injection.ResultsIn rodent cachexia, we found progressively higher numbers of CD68+ myeloid cells in the liver along cancer‐cachexia development. Similar findings are described for CC, whose liver showed infiltration of the same cell type, compared with both WSC and control patient organs. In advanced rodent cachexia, hepatic phosphorylated c‐Jun N‐terminal kinase protein content and the inflammasome pathway protein expression were increased in relation to baseline (P < 0.05). These changes were accompanied by augmented expression of the active interleukin‐1β (IL‐1β) form (P < 0.05 for both circulating and hepatic content).ConclusionsThe results show that cancer cachexia is associated with an increase in the number of myeloid cells in rodent and human liver and with modulation of hepatic inflammasome pathway. The latter contributes to the aggravation of systemic inflammation, through increased release of IL‐1β.
Small extracellular vesicles are released ex vivo from platelets into serum and from residual blood cells into stored plasma
Authors: Małys Małgorzata S. , Köller Maximilian C. , Papp Kristin , Aigner Christof , Dioso Daffodil , Mucher Patrick , Schachner Helga , Bonelli Michael , Haslacher Helmuth , Rees Andrew J. , Kain Renate 
Published: May 2023

AbstractSmall extracellular vesicles (sEV) purified from blood have great potential clinically as biomarkers for systemic disease; however interpretation is complicated by release of sEV ex vivo after blood taking. To quantify the problem and devise ways to minimise it, we characterised sEV in paired serum, plasma and platelet poor plasma (PPP) samples from healthy donors. Immunoblotting showed twofold greater abundance of CD9 in sEV fractions from fresh serum than from fresh plasma or PPP. MACSPlex confirmed this, and showed that proteins expressed on platelet sEV, either exclusively (CD41b, CD42a and CD62P) or more widely (HLA‐ABC, CD24, CD29 and CD31) were also twofold more abundant; by contrast non‐platelet proteins (including CD81) were no different. Storage of plasma (but not serum) increased abundance of platelet and selected leukocyte sEV proteins to at least that of serum, and this could be recapitulated by activating cells in fresh plasma by Ca2+, an effect abrogated in PPP. This suggests that a substantial proportion of sEV in serum and stored plasma were generated ex vivo, which is not the case for fresh plasma or PPP. Thus we provide strategies to minimise ex vivo sEV generation and criteria for identifying those that were present in vivo.