bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of temperature degradation on the low velocity impact behavior of hybrid thermoplastic PEEK laminates
Authors: Vieille B , Coppalle A 
Published: April 2023

To evaluate the severity of thermal degradation on the impact response of carbon (C) and glass (G) fibers reinforced PolyEther Ether Ketone (PEEK) laminates, low velocity impact tests were conducted at a temperature higher than the glass transition temperature Tg (150°C) and after exposure to a kerosene flame (5-10-15′). The first important effect resulting from temperature increase was a reduction of the impact energy required to induce BVID (Barely Visible Impact Damage). The second effect was that matrix ductility (enhanced at T>Tg) contributes to significantly modify the permanent indentation. Not surprisingly, the plastic and viscoplastic deformation mechanisms being ruled by the PEEK matrix behavior at high temperature, the permanent indentation increases by almost 40% for all impact energies. Contrary to the external damage represented by permanent indentation, temperature has a tremendous influence on the internal damage as there was virtually no delamination in the CG/PEEK laminates impacted at Room Temperature (RT) and 150°C. At last, impact tests conducted on specimens exposed to a kerosene flame implied that the impact bearing capabilities of CG/PEEK laminates dramatically decreased after a 5′ exposure and became even critical after 10′ as perforation was observed. For 40 J impacts, the permanent indentation was multiplied by 3–9 with respect to the as-received specimens.
Outcome of premarital genetic counseling for couples at risk of hemoglobinopathies in Kingdom of Bahrain
Authors: Bahram Samya , Haji Aalaa , Abdulwahab Hawra , Mohsen Hanan , Alnashaba Tahera , Al-Aradi Zainab , Mandeel Mohamed 
Published: April 2023

Objectives Hemoglobinopathies are the commonest inherited blood disorders and form a serious burden worldwide, affecting communities, patient quality of life and healthcare resources. The Kingdom of Bahrain has issued a law obligating couples to undergo premarital screening to detect those at risk of having children affected with these disorders. The aim of this study was to analyze the marital decisions of couples at risk for hemoglobinopathies and follow up the outcomes. Methods A retrospective study was conducted on couples at risk for hemoglobinopathies identified during the premarital screening program at local health centers in the Kingdom of Bahrain and referred to the genetics department in the Salmaniya Medical Complex for genetic counselling in 2018–2020. Results A total of 189 couples were found to be at risk for hemoglobinopathies, of whom 159 completed the survey. Of these, 107 (67%) decided to proceed with their marriage and 26 couples achieved pregnancy. Out of 24 at-risk pregnancies with known outcome, 83.3% were spontaneous whereas only 16.7% underwent in-vitro fertilization (IVF) with preimplantation genetic diagnosis (PGD). Eight out of 20 infants born to couples after spontaneous conception were affected. A positive attitude toward IVF with PGD was held by 60% of at-risk couples. Conclusions Despite undergoing premarital screening and genetic counselling, a large percentage of at-risk couples proceeded with their marriage. Most of them justified their decision due to the availability of advanced methods that aid in the prevention of having an affected child. However, the cost of such intervention was a major barrier for the majority of couples.
A Descriptive Study to Assess Level of Stress, Anxiety, and Pain Among Hospitalized Children Admitted in the Pediatric Surgery Unit of APC, PGIMER, Chandigarh 2020−2022
Authors: Praveen Shama , Sharma Sunita , Peters Nitin James 
Published: April 2023

Background Hospitalization is stressful for children. Hospitalized children suffer from stress due to the negative change in their life. Children facing medical procedures often experience pain and anxiety. Physical, psychological, social, cultural, and environmental factors all influence child behavior, which is especially challenging for children as compared to adults. Both pharmacological and non-pharmacological approaches can be used to manage distress among children. There are several ways to reduce stress, anxiety, and pain among hospitalized children. Puzzles, books, comics, crossword, video games, televisions, toys, phone, and so on. all are effective ways through which child can be engaged. Aim To assess the level of stress, anxiety, and pain among hospitalized children admitted in the Pediatric Surgery Unit, APC, PGIMER, Chandigarh, India. Methods A descriptive study was conducted on 60 hospitalized children. Purposive sampling was used to recruit the sample. The data were collected through interview schedule consisting of socio-demographic profile, clinical profile, and questionnaire on stress, anxiety and pain. Results The findings of the study revealed that all the children were having moderate stress, whereas majority of the children (91.7%) were having moderate anxiety, and only 8.3% of the children were having severe anxiety. During the assessment it was also found that almost 55% were having pain score 4, that is, Hurts little bit as assessed by Wong baker face pain scale. Conclusion Children who were being hospitalized were having varied degrees of stress, anxiety, and pain. Therefore, some diversional therapy should be planned to reduce their distress.
Do live-stream telemedicine follow-up appointments for a Ponseti-treated clubfoot caseload compare favourably with a face-to-face review? Observed clinical outcomes and treatment provision during the COVID-19 pandemic
Authors: Douglas Christine , Simmonds Jane , Kedroff Louise , Tennant Sally 
Published: April 2023

Introduction Restrictions on face-to-face (F2F) healthcare services during the recent COVID-19 pandemic necessitated novel provision of care for Ponseti-treated clubfoot patients. This retrospective review compares the effectiveness of telemedicine (TM) using live-stream videoconferencing compared to conventional F2F review, in two cohorts of 78 patients attending routine follow-up, during Ponseti-treated clubfoot bracing in their first 5 years. Methods Rates of compliance, recurrence of deformity, and type of intervention provided were compared between cohorts. The TM cohort was re-evaluated F2F as part of routine follow-up care. Attendance rates were compared between cohorts and with an equivalent time period the previous year. Results There was no significant difference in the rate of compliance between cohorts (77% in the TM group, 74% in the F2F group), or in the rate of recurrence. Subsequent F2F review of the TM cohort showed that compliance had improved in some patients, indicating successful TM intervention. There were no missed cases of recurrence. TM follow-up appointments offered similar rates of management of skin problems, brace adjustment, and provision of exercises. At least one element of intervention was provided in 64% of the TM group, and 72% of the F2F group. TM connection was successful in 74% of booked appointments. The number of patients attending was similar to F2F bookings one year prior. Discussion The use of TM for routine follow-up of Ponseti-treated clubfoot patients can be as clinically effective as F2F assessment, and has potential for integration into routine follow-up care.
The effect of patient empowerment on patient activation level: A review of individuals with cardiovascular diseases
Authors: Düzel Barış , Saygili Meltem , Özer Özlem , Zubaroğlu Yanardağ Melek 
Published: April 2023

Objectives The aim of this study is to examine the effect of patient empowerment on patient activation level in individuals with cardiovascular diseases. Methods The population of the study is adult individuals receiving inpatient treatment in the Cardiology clinic of a city hospital operating in Turkey. In the study, convenience sampling method was applied, and a questionnaire was conducted from 543 patients. Results As a result of the analyses made in the study, positive and low correlations were detected between the patient empowerment scale sub-dimensions and the patient activation level. According to regression analysis results, it was identified that the patient empowerment sub-dimensions together accounted for 6.4% of the total variance on the patient activation level, and the increase in the ‘knowledge and understanding’ levels of the patients statistically increased their perceptions of the patient activation level. Discussion The results show that by providing patient empowerment in individuals with chronic diseases, their active participation can be increased in the treatment processes and consequently in the chronic disease management.
Rehabilitation following flexor tendon injury in Zone 2: a randomized controlled study
Authors: Renberg Markus , Turesson Christina , Borén Linda , Nyman Erika , Farnebo Simon 
Published: April 2023

The aim of this study was to compare an early active motion (EAM) regimen to a modified Kleinert passive motion therapy in Zone 2 flexor tendon injuries with regards to range of motion (ROM), grip strength and patient-reported outcome measures (PROMs). Seventy-two patients were included. At 3 months postoperatively, we found no difference in total active motion (TAM) between the EAM and the Kleinert groups (median 195.5°, range 115°–273° versus median 191.5°, range 113°–260°), but a significantly better grip strength (median 76%, range 44%–99% versus median 54%, range 19%–101%; p <  0.0005) in the EAM group. Disabilities of the Arm, Shoulder and Hand (DASH) score as well as patient-reported weakness, cold intolerance and problems in daily activities also favoured the EAM group. At 12 months postoperatively, there was no difference in TAM, grip strength or any of the PROMs used. We conclude that EAM leads to a quicker recovery in terms of grip strength and PROMs, but that both regimens lead to similar results at 12 months. Level of evidence: I
Oxygen insufflation through the bronchoscope channel for sedation-induced hypoxia: safe and effective
Authors: Abouzgheib Wissam , Miller Henry , Bartter Thaddeus 
Published: April 2023

Objectives: To evaluate both efficacy and safety parameters for insufflation through the bronchoscope as a method of recovery from sedation-induced hypoxia. To explore parameters applicable to use in human beings using an animal model. Materials and methods: Two adult pigs were sedated enough to depress respiratory drive. The effects of insufflation at 15 l/min (the upper limits of flow that might be used clinically) were then evaluated. Pressure and volume responses to bronchoscopy during intubation and without an endotracheal tube in place were recorded. Several assays were performed for each scenario, with each animal acting as its own control. Recovery from hypoxemia using insufflation was compared with recovery using mechanical ventilation. Results: Insufflation was effective, with rapid increases in fraction of inspired oxygen (FIO2), saturation, and partial pressure of arterial oxygen (PaO2). The rate of recovery using insufflation was faster than that from institution of mechanical ventilation. Insufflation in an intubated animal with cuff inflated led to a rapid and dangerous rise in pressure. With balloon deflated, there were no adverse pressure consequences from insufflation via the endotracheal tube at a rate of 15 l/min. Conclusion: Insufflation through the bronchoscope for episodes of sedation-induced hypoxia should be safe and effective as long as not delivered within a closed system.
Perioperative Insulin Regimens in Patients With Insulin-Treated Type 2 Diabetes Mellitus Hospitalized for a Short Time for Minor Eye Surgery
Authors: Pfleger Sandra , Meçani Renald , Semlitsch Barbara , Krall Anja , Sendlhofer Gerald , Mader Julia K. , Wedrich Andreas 
Published: April 2023

Background: Elective surgery in patients with insulin-treated type 2 diabetes mellitus (T2D) and the admission period in the hospital, comprise a distinctive and challenging situation for physicians, nurses, as well as for the patients themselves. There is a lack of widely accepted evidence-based and standardized approach of care in regard to perioperative management of patients with insulin-treated T2D. Methods: The main purpose of this proof-of-concept study was to investigate whether a standardized insulin and meal regimen on the day of surgery leads to a better management of diabetes in terms of blood glucose (BG) levels. Two different insulin and meal regimens—group A with half of insulin dose given with a standardized postoperative meal and group B with a custom preoperative breakfast and full insulin dose—were compared with Group C with routine care (no meal and no insulin injection on the day of surgery). Each group consisted of 12 to 15 patients. BG measurements were performed pre- and immediately postoperatively, before meals and at bedtime. Results: Both standardized and well-defined insulin and meal regimens resulted in better average BG levels in the perioperative period, especially in the morning after the surgery. Conclusions: In this study, we observed that a standardized perioperative insulin regimen efficiently lowered postoperative BG levels. Providing a custom breakfast and a full insulin dose resulted in lower postoperative BG levels. These approaches were not associated with an increase in hypoglycemic events. Physicians and nursing staff gave positive feedback to the structured and well-defined approaches.
Human herpesvirus 6–specific T-cell immunity in allogeneic hematopoietic stem cell transplant recipients
Authors: Noviello Maddalena , Lorentino Francesca , Xue Elisabetta , Racca Sara , Furnari Giulia , Valtolina Veronica , Campodonico Edoardo , Dvir Roee , Lupo-Stanghellini Maria Teresa , Giglio Fabio , Piemontese Simona , Clerici Daniela , Oltolini Chiara , Tassi Elena , Beretta Valeria , Farina Francesca , Mannina Daniele , Ardemagni Anna , Vago Luca , Bernardi Massimo , Corti Consuelo , Peccatori Jacopo , Clementi Massimo , Ciceri Fabio , Bonini Chiara , Greco Raffaella 
Published: April 2023

Abstract
Human herpesvirus 6 (HHV-6) can reactivate after allogeneic hematopoietic stem cell transplant (allo-HSCT) and may lead to severe symptoms. HHV-6–specific immune responses after HSCT are largely unexplored. We conducted a prospective observational study on 208 consecutive adult patients who received allo-HSCT to investigate HHV-6 reactivations and specific immune responses. Interferon gamma–producing HHV-6–specific T cells were quantified using enzyme-linked immunospot assay (ELISpot). HHV-6 reactivation occurred in 63% of patients, at a median of 25 days from allo-HSCT. Only 40% of these presented a clinically relevant infection, defined by the presence of classical HHV-6 end-organ diseases (EODs), based on European Conference on Infections in Leukaemia (ECIL) guidelines, and other possible HHV6-related EODs. Using multivariate analysis, we identified risk factors for HHV-6 reactivation: previous allo-HSCT, posttransplant cyclophosphamide (PT-Cy), and time-dependent steroids introduction. The use of PT-Cy and steroids were associated with clinically relevant infections, whereas higher CD3+ cell counts seemed to be protective. Interestingly, circulating HHV-6–specific T cells were significantly higher in patients with reactivated virus. Moreover, HHV-6–specific T-cell responses, quantified at &gt;4 days after the first viremia detection, predicted clinically relevant infections (P &lt; .0001), with higher specificity (93%) and sensitivity (79%) than polyclonal CD3+ cells per μL. Overall survival and transplant-related mortality were not affected by time-dependent HHV-6 reactivation, whereas a significant association was observed between clinically relevant infections and acute graft-versus-host disease. These results shed light on the role of HHV-6 in allo-HSCT and may affect HHV-6 monitoring and treatment.
Monocytes reprogrammed by tumor microparticle vaccine inhibit tumorigenesis and tumor development
Authors: Sun Weiwei , Dai Lili , Cao Yuqing , Pan Pengtao , Zhi Lijuan , Wang Xinke , Yuan Xinzhong , Gao Zi , Guo Sheng , Liu Guoyan , Yin Junlei , Xie Liangliang , Wang Liping , Wang Yanling , Li Wensheng , Li Hong , Jia Yunjie 
Published: April 2023

AbstractTumor microparticles (T-MPs) are considered as a tumor vaccine candidate. Although some studies have analyzed the mechanism of T-MPs as tumor vaccine, we still lack understanding of how T-MPs stimulate a strong anti-tumor immune response. Here, we show that T-MPs induce macrophages to release a key chemotactic factor CCL2, which attracts monocytes to the vaccine injection site and enhances endocytosis of antigen. Monocytes subsequently enter the draining lymph node, and differentiate into monocyte-derived DCs (moDCs), which present tumor antigens to T lymphocytes and deliver a potent anti-tumor immune response. Mechanically, T-MPs activate the cGAS-STING signaling through DNA fragments, and then induce monocytes to upregulate the expression of IRF4, which is a key factor for monocyte differentiation into moDCs. More importantly, monocytes that have endocytosed T-MPs acquire the ability to treat tumors. Collectively, this work might provide novel vaccination strategy for the development of tumor vaccines and facilitate the application of T-MPs for clinic oncotherapy.
Bacterial outer membrane vesicles as cationic dye carriers for optoacoustics-guided phototherapy of cancer
Authors: Liu Nian , Gujrati Vipul , Werner Juan Pablo Fuenzalida , Mishra Kanuj , Anzenhofer Pia , Stiel Andre C. , Mettenleiter Gabriele , Feuchtinger Annette , Walch Axel , Ntziachristos Vasilis 
Published: April 2023

Abstract
Background
Cationic dyes are widely used as biomarkers for optical imaging. However, most of these are hydrophobic and cannot be employed in vivo without chemical conjugation or modification. Herein, we report for the first time the use of bacterial outer membrane vesicles (OMVs) as nanocarriers of cationic dyes for cancer theranostics.

Results
We demonstrate that cationic dyes (IR780, Cy7, and Cy7.5) form stable complexes with negatively charged bacterial-OMVs, improving the dyes’ in vivo circulation and optoacoustic properties. Such OMV-Dye complexes are biodegradable and safe for in vivo applications. Importantly, this method of cationic dye loading is faster and easier than synthetic chemistry approaches, and the efficient tumor accumulation of OMV-Dyes enables sensitive tumor detection using optoacoustic technology. As a proof-of-concept, we generated OMV-IR780 for optoacoustics-guided in vivo tumor phototherapy in a mouse model.

Conclusions
Our results demonstrate cationic dye-bound OMVs as promising novel nanoagents for tumor theranostics.
EZH2 as a prognostic-related biomarker in lung adenocarcinoma correlating with cell cycle and immune infiltrates
Authors: Fan Kui , Zhang Bo-hui , Han Deng , Sun Yun-chuan 
Published: April 2023

Abstract
Backgrounds
It has been observed that high levels of enhancer of zeste homolog 2 (EZH2) expression are associated with unsatisfactory prognoses and can be found in a wide range of malignancies. However, the effects of EZH2 on Lung Adenocarcinoma (LUAD) remain elusive. Through the integration of bioinformatic analyses, the present paper sought to ascertain the effects of EZH2 in LUAD.

Methods
The TIMER and UALCAN databases were applied to analyze mRNA and protein expression data for EZH2 in LUAD. The result of immunohistochemistry was obtained from the HPA database, and the survival curve was drawn according to the library provided by the HPA database. The LinkedOmics database was utilized to investigate the co-expressed genes and signal transduction pathways with EZH2. Up- and down-regulated genes from The Linked Omics database were introduced to the CMap database to predict potential drug targets for LUAD using the CMap database. The association between EZH2 and cancer-infiltrating immunocytes was studied through TIMER and TISIDB. In addition, this paper explores the relationship between EZH2 mRNA expression and NSCLC OS using the Kaplan–Meier plotter database to further validate and complement the research. Furthermore, the correlation between EZH2 expression and EGFR genes, KRAS genes, BRAF genes, and smoking from the Cancer Genome Atlas (TCGA) database is analyzed.

Results
In contrast to paracancer specimens, the mRNA and protein levels of EZH2 were higher in LUAD tissues. Significantly, high levels of EZH2 were associated with unsatisfactory prognoses in LUAD patients. Additionally, the coexpressed genes of EZH2 were predominantly associated with numerous cell growth-associated pathways, including the cell cycle, DNA replication, RNA transport, and the p53 signaling pathway, according to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. The results of TCGA database revealed that the expression of EZH2 was lower in normal tissues than in lung cancer tissues (p < 0.05). Smoking was associated with elevated EZH2 expression (p < 0.001). EZH2 was highly expressed in lung cancers with positive KRAS expression, and the correlation was significant in lung adenocarcinoma (r = 0.3129, p < 0.001). CMap was applied to determine the top 15 positively correlated drugs/molecules and the top 15 negatively correlated drugs/molecules. MK-1775, MK-5108, fenbendazole, albendazole, BAY-K8644, evodiamine, purvalanol-a, mycophenolic-acid, PHA-793887, and cyclopamine are potential drugs for patients with lung adenocarcinoma and high EZH2 expression.

Conclusions
Highly expressed EZH2 is a predictor of a suboptimal prognosis in LUAD and may serve as a prognostic marker and target gene for LUAD. The underlying cause may be associated with the synergistic effect of KRAS, immune cell infiltration, and metabolic processes.
Drug-target interaction prediction based on spatial consistency constraint and graph convolutional autoencoder
Authors: Chen Peng , Zheng Haoran 
Published: April 2023

Abstract
Background
Drug-target interaction (DTI) prediction plays an important role in drug discovery and repositioning. However, most of the computational methods used for identifying relevant DTIs do not consider the invariance of the nearest neighbour relationships between drugs or targets. In other words, they do not take into account the invariance of the topological relationships between nodes during representation learning. It may limit the performance of the DTI prediction methods.

Results
Here, we propose a novel graph convolutional autoencoder-based model, named SDGAE, to predict DTIs. As the graph convolutional network cannot handle isolated nodes in a network, a pre-processing step was applied to reduce the number of isolated nodes in the heterogeneous network and facilitate effective exploitation of the graph convolutional network. By maintaining the graph structure during representation learning, the nearest neighbour relationships between nodes in the embedding space remained as close as possible to the original space.

Conclusions
Overall, we demonstrated that SDGAE can automatically learn more informative and robust feature vectors of drugs and targets, thus exhibiting significantly improved predictive accuracy for DTIs.