bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decay of HIV RNA in Seminal Plasma and Rectal Fluid in Treatment-Naive Adults Starting Antiretroviral Therapy With Dolutegravir Plus Lamivudine or Bictegravir/Emtricitabine/Tenofovir Alafenamide
Authors: Scévola Sofía , Niubó Jordi , Domingo Pere , Verdejo Guillermo , Curran Adrian , Diaz-Brito Vicens , Peñafiel Judith , Tiraboschi Juan , Morenilla Sandra , Garcia Benito , Soriano Irene , Podzamczer Daniel , Imaz Arkaitz 
Published: August 2023

Abstract

Background
Decay of HIV in seminal plasma (SP) and rectal fluid (RF) has not yet been described for the antiretroviral combination of dolutegravir (DTG) + lamivudine (3TC).

Methods
In this randomized multicenter pilot trial, males who were antiretroviral naive were randomized (2:1) to DTG + 3TC or bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF). HIV-1 RNA was measured in blood plasma (BP), SP, and RF at baseline; days 3, 7, 14, and 28; and weeks 12 and 24.

Results
Of 25 individuals enrolled, 24 completed the study (DTG + 3TC, n = 16; BIC/FTC/TAF, n = 8). No significant differences were observed between groups for median decline in HIV-1 RNA from baseline at each time point or median time to achieve HIV-1 RNA &lt;20 copies/mL in BP and SP and &lt;20 copies/swab in RF. HIV-1 RNA decay patterns were compared in individuals receiving DTG + 3TC. Despite significantly higher percentages for changes from baseline in BP, median (IQR) times to HIV-1 RNA suppression were shorter in SP (7 days; 0–8.75) and RF (10.5 days; 3–17.5) than in BP (28 days; 14–84; P &lt; .001).

Conclusions
Comparable HIV-1 RNA decay in BP, SP, and RF was observed between DTG + 3TC and BIC/FTC/TAF. As shown with triple-drug integrase inhibitor–based regimens, rapid HIV-1 RNA suppression in SP and RF is achieved with DTG + 3TC, despite decay patterns differing from those of BP.

Clinical Trials Registration
EudraCT 2019-004109-28.
Association of ward-level antibiotic consumption with healthcare-associated Clostridioides difficile infections: an ecological study in five German university hospitals, 2017–2019
Authors: Rohde Anna M , Mischnik Alexander , Behnke Michael , Dinkelacker Ariane , Eisenbeis Simone , Falgenhauer Jane , Gastmeier Petra , Häcker Georg , Herold Susanne , Imirzalioglu Can , Käding Nadja , Kramme Evelyn , Peter Silke , Piepenbrock Ellen , Rupp Jan , Schneider Christian , Schwab Frank , Seifert Harald , Steib-Bauert Michaela , Tacconelli Evelina , Trauth Janina , Vehreschild Maria J G T , Walker Sarah V , Kern Winfried V , Jazmati Nathalie ,   DZIF R-NET Study Group, Biehl Lena , Braun Jochen , Buhl Michael , Chakraborty Trinad , Fitzenwanker Moritz , Gölz Hanna , Grundmann Hajo , Hennelly Catriona , Hölzl Florian , Kohlmorgen Britta , Künstle Larissa , Lang Alexandra , Lengler Azita , Lenke Dana , Diaz Luis Alberto Peña , Pilarski Georg , Proske Susanna , Schmiedel Judith , Thoma Norbert , Walinski Bianca 
Published: August 2023

Abstract

Objectives
To analyse the influence of antibiotic consumption on healthcare-associated healthcare onset (HAHO) Clostridioides difficile infection (CDI) in a German university hospital setting.

Methods
Monthly ward-level antibiotic consumption measured in DDD/100 patient days (pd) and CDI surveillance data from five university hospitals in the period 2017 through 2019 were analysed. Uni- and multivariable analyses were performed with generalized estimating equation models.

Results
A total of 225 wards with 7347 surveillance months and 4 036 602 pd participated. With 1184 HAHO-CDI cases, there was a median incidence density of 0.17/1000 pd (IQR 0.03–0.43) across all specialties, with substantial differences among specialties. Haematology-oncology wards showed the highest median incidence density (0.67/1000 pd, IQR 0.44–1.01), followed by medical ICUs (0.45/1000 pd, IQR 0.27–0.73) and medical general wards (0.32/1000 pd, IQR 0.18–0.53). Multivariable analysis revealed carbapenem (mostly meropenem) consumption to be the only antibiotic class associated with increased HAHO-CDI incidence density. Each carbapenem DDD/100 pd administered increased the HAHO-CDI incidence density by 1.3% [incidence rate ratio (IRR) 1.013; 95% CI 1.006–1.019]. Specialty-specific analyses showed this influence only to be valid for haematological-oncological wards. Overall, factors like ward specialty (e.g. haematology-oncology ward IRR 2.961, 95% CI 2.203–3.980) or other CDI cases on ward had a stronger influence on HAHO-CDI incidence density (e.g. community-associated CDI or unknown association case in same month IRR 1.476, 95% CI 1.242–1.755) than antibiotic consumption.

Conclusions
In the German university hospital setting, monthly ward-level carbapenem consumption seems to increase the HAHO-CDI incidence density predominantly on haematological-oncological wards. Furthermore, other patient-specific factors seem to be equally important to control HAHO-CDI.
Evaluation of branched-chain amino acid interactions in 10 to 20 kg nursery pigs using a central composite design
Authors: Humphrey Dalton C , Haydon Keith , Greiner Laura L 
Published: August 2023

Abstract
Two groups of 240 pigs (PIC 337 × 1050, PIC Genus, Hendersonville, TN) were used to investigate the interactions between leucine, isoleucine, and valine on the growth performance of approximately 10 to 20 kg nursery pigs. At weaning, pigs were placed into 40 pens with three barrows and three gilts per pen and fed a common diet for 3 wk. On day 21 postweaning, pens were randomly assigned to 1 of 15 dietary treatments in a central composite design. Diets were formulated to various levels of standardized ileal digestible (SID) Leu, Ile, and Val by supplementing L-Leu, L-Ile, and L-Val. Levels of the branched-chain amino acids, expressed as ratios to SID Lys, ranged from 98% to 180%, 46% to 64%, and 51% to 78% for Leu, Ile, and Val, respectively. Diets were formulated to be iso-Lys, isonitrogenous, and isocaloric. Pig weights and feed intake were measured for the 21-d experiment to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (G:F). Growth performance data were analyzed using the lm() function in R version 4.2.2 (R Core Team, 2022). The second-order polynomial model included the linear and quadratic effects of Leu, Ile, and Val, their three two-way interactions, and initial body weight. Pen was the experimental unit, and parameters were considered significant at P ≤ 0.10. A linear and quadratic effect of Val was observed for ADG and G:F (P &lt; 0.001). There was an interaction between Leu and Ile for ADG (P = 0.069) and G:F (P = 0.032), where increasing Leu and decreasing Ile, and the inverse, improved ADG and G:F. However, growth and efficiency were negatively impacted as Leu and Ile increased in the diet. There was an interaction between Leu and Val for ADFI (P = 0.060), where Leu negatively impacted feed intake at low levels of Val but had little impact as Val increased above NRC (2012) recommendations.
In conclusion, Val linearly and quadratically impacted ADG and G:F, regardless of Leu and Ile levels in the diet, while ADG and G:F were reduced with high levels of Leu and Ile, which was resolved as either Leu or Ile was reduced. Furthermore, ADFI was negatively impacted by increased Leu when Val was below NRC (2012) recommendations but was not affected by Leu at higher Val levels. Together, the results of this experiment emphasize the complexity of amino acid metabolism in nursery pigs and the importance of considering potential interactions among amino acids when conducting requirement studies.
The stability of NPM1 oligomers regulated by acidic disordered regions controls the quality of liquid droplets
Authors: Okuwaki Mitsuru , Ozawa Shin-Ichiro , Ebine Shuhei , Juichi Motoki , Umeki Tadanobu , Niioka Kazuki , Kikuchi Taiyo , Tanaka Nobutada 
Published: August 2023

Abstract
The nucleolus is a membrane-less nuclear body that typically forms through the process of liquid–liquid phase separation (LLPS) involving its components. NPM1 drives LLPS within the nucleolus and its oligomer formation and inter-oligomer interactions play a cooperative role in inducing LLPS. However, the molecular mechanism underlaying the regulation of liquid droplet quality formed by NPM1 remains poorly understood. In this study, we demonstrate that the N-terminal and central acidic residues within the intrinsically disordered regions (IDR) of NPM1 contribute to attenuating oligomer stability, although differences in the oligomer stability were observed only under stringent conditions. Furthermore, the impact of the IDRs is augmented by an increase in net negative charges resulting from phosphorylation within the IDRs. Significantly, we observed an increase in fluidity of liquid droplets formed by NPM1 with decreased oligomer stability. These results indicate that the difference in oligomer stability only observed biochemically under stringent conditions has a significant impact on liquid droplet quality formed by NPM1. Our findings provide new mechanistic insights into the regulation of nucleolar dynamics during the cell cycle.
Naringenin ameliorates vascular senescence and atherosclerosis involving SIRT1 activation
Authors: Wang Jie , Wu Ruoman , Hua Yiqiao , Ling Shijia , Xu Xiaole 
Published: August 2023

Abstract

Objectives
This study was to explore the potential effects and mechanism of naringenin against vascular senescence in atherosclerosis focusing on the SIRT1-mediated signalling pathway.

Methods
Aged apoE−/− mice were administrated with naringenin continuously for three months. Lipid parameters in serum and pathological changes and associated protein expression in aorta were examined. In vitro, endothelial cells were treated with H2O2 to induce senescence.

Key findings
Dyslipidemia, atherosclerotic lesion formation and vascular senescence were found in ApoE−/− mice, which were significantly ameliorated by naringenin treatment. Naringenin decreased reactive oxygen species overproduction and enhanced the activities of antioxidant enzymes in aorta. It also decreased mitoROS production and increased protein expressions of mitochondrial biogenesis-related genes in aorta. Moreover, naringenin treatment enhanced aortic protein expression and activity of SIRT1. Meanwhile, naringenin increased deacetylation and protein expression of SIRT1’s target genes FOXO3a and PGC1α. In vitro study, the benefits of naringenin on endothelial senescence, oxidative stress and mitochondrial injury as well as protein expressions and acetylated levels of FOXO3a and PGC1α were diminished in cells transfected with SIRT1 siRNA.

Conclusions
Naringenin could ameliorate vascular senescence and atherosclerosis and the activation of SIRT1, with subsequent deacetylation and regulation of FOXO3a and PGC1α, is involved in this process.
Stefano Franscini: The statistician who built a nation
Authors: Grüninger Servan 
Published: August 2023

Abstract
Modern Switzerland was founded 175 years ago. However, despite being connected by a constitution, the new country remained divided by language, religion, and history – a state but not a nation. It is thanks to the statistician and politician Stefano Franscini that statistics came to play an important role in forging a common Swiss identity. Servan Grüninger tells his story
The way you make me feel: an ecological momentary assessment study on couple similarity in sexual arousal and its sexual, relational, and affective correlates
Authors: Pawłowska Aleksandra , Janssen Erick , Dewitte Marieke 
Published: August 2023

Abstract

Background
Although sexual arousal is commonly experienced in the daily context of relationships, most of what we know about sexual arousal comes from studies on individuals, often conducted in a laboratory context.

Aim
To explore to what extent similarity in levels of sexual arousal during nongenital physical intimacy (ie, cuddling and kissing) was associated with each partner’s affect as well as sexual and relationship satisfaction.

Methods
Ninety-four cohabitating couples (mean ± SD age, 26.30 ± 7.60; 88 mixed gender, 6 same gender) completed 6 ecological momentary assessments a day for 10 consecutive days. We used response surface analysis to examine the associations among the degree and direction of similarity in partners’ sexual arousal and affective, sexual, and relational outcome variables.

Outcomes
Sexual satisfaction, relationship satisfaction, and positive and negative affect.

Results
Sexual arousal levels covaried only when partners engaged in physically intimate behaviors, unlike affective responses, which covaried within couples more globally over time. Within-couple similarity at high levels of sexual arousal was positively associated with women’s sexual satisfaction but unrelated to men and women’s relationship satisfaction and affect. Individual- and couple-level sexual arousal was positively associated with men’s sexual satisfaction and women’s sexual and relationship satisfaction. Couple-level sexual arousal was relevant to men’s affect such that positive affect was higher when sexual arousal levels within the couple were high. Our analyses also revealed a discrepancy effect in that women’s positive affect was higher when their own sexual arousal levels were higher than those of their partners.

Clinical Implications
These findings suggest that as long as sexual arousal levels within a couple are sufficiently high, sexual arousal similarity and discrepancy can be beneficial to one’s well-being, supporting the relevance of therapeutical techniques aimed at increasing arousal levels to promote a better affective and relational climate for couples.

Strengths and Limitations
This study is the first to test the daily associations among sexual arousal similarity and its correlates in a sample of cohabitating couples, providing a more comprehensive view of the interpersonal dynamics through which sexual arousal may influence individual, relational, and sexual well-being. Given our sample’s relatively young age, as well as high sexual and relationship satisfaction, the results may not generalize to couples experiencing sexual or relational distress.

Conclusion
Within the context of daily relationships, individual- and couple-level dynamics of sexual arousal were associated with sexual and relationship satisfaction, as well as with affective responses of relationship partners.
Ethylene inhibits ABA-induced stomatal closure via regulating NtMYB184-mediated flavonol biosynthesis in tobacco
Authors: Song Zhongbang , Zhao Lu , Ma Wenna , Peng Zhongping , Shi Junli , Pan Feng , Gao Yulong , Sui Xueyi , Rengel Zed , Chen Qi , Wang Bingwu 
Published: August 2023

Abstract
Stomatal movement can be regulated by ABA signaling through synthesis of reactive oxygen species (ROS) in guard cells. By contrast, ethylene triggers the biosynthesis of antioxidant flavonols to suppress ROS accumulation and prevent ABA-induced stomatal closure; however, the underlying mechanism remains largely unknown. In this study, we isolated and characterized the tobacco (Nicotiana tabacum) R2R3-MYB transcription factor NtMYB184, which belongs to the flavonol-specific SG7 subgroup. RNAi suppression and CRISPR/Cas9 mutation (myb184) of NtMYB184 in tobacco caused down-regulation of flavonol biosynthetic genes and decreased the concentration of flavonols in the leaves. Yeast one-hybrid assays, transactivation assays, EMSAs, and ChIP-qPCR demonstrated that NtMYB184 specifically binds to the promoters of flavonol biosynthetic genes via MYBPLANT motifs. NtMYB184 regulated flavonol biosynthesis in guard cells to modulate ROS homeostasis and stomatal aperture. ABA-induced ROS production was accompanied by the suppression of NtMYB184 and flavonol biosynthesis, which may accelerate ABA-induced stomatal closure. Furthermore, ethylene stimulated NtMYB184 expression and flavonol biosynthesis to suppress ROS accumulation and curb ABA-induced stomatal closure. In myb184, however, neither the flavonol and ROS concentrations nor the stomatal aperture varied between the ABA and ABA+ethylene treatments, indicating that NtMYB184 was indispensable for the antagonism between ethylene and ABA via regulating flavonol and ROS concentrations in the guard cells.
The exclusion of anti-D alloantibody in a suspected anti-G antibody in a pregnant 28-year-old Odiya Indian woman
Authors: Nivedita Kella , Mukherjee Somnath , Prakash Satya , Sahu Ansuman , Mishra Debasish 
Published: August 2023

Abstract
The Rh-D negative pregnancy is commonly associated with alloimmunization against D-antigen. It can be prevented by anti-D prophylaxis in pregnant patients with negative results on antibody screening. Hence, it is essential to exclude alloantibody-D in the presence of multiple alloantibodies. Anti-G antibody is formed after exposure to G antigen in neonate RBCs. Blood-group discrepancy was noted in reverse grouping, and antibody-screening results were positive in our case individual, a 28-year old Odiya Indian woman. We performed antibody identification on serum specimens from this patient, which revealed the pattern of anti-D + anti-C antibody specificity. Blood-group discrepancy was solved using rr (ce/ce)–phenotype pooled cells for reverse grouping. We identified anti-G antibodies by themselves without anti-D and anti-C after performing sequential adsorption of serum with rʹrʹ (Ce/Ce) and R2R2 (DcE/DcE) group-O RBCs in the mother, who had rr phenotype and primigravida designation. After completing antibody screening at the first antenatal check-up, we recommended prophylactic anti-D for the mother in any future pregnancies she may have.
Approaches to nonlinear curve fitting in laboratory medicine
Authors: McPherson Peter A C 
Published: August 2023

Abstract
Nonlinear curve fitting is an important process in laboratory medicine, particularly with the increased use of highly sensitive antibody-based assays. Although the process is often automated in commercially available software, it is important that clinical scientists and physicians recognize the limitations of the various approaches used and are able to select the most appropriate model. This article summarizes the key nonlinear functions and demonstrates their application to common laboratory data. Following this, a basic overview of the statistical comparison of models is presented and then a discussion of important algorithms used in nonlinear curve fitting. An accompanying Microsoft Excel workbook is available that can be used to explore the content of this article.
MUSE–ALMA Haloes – IX. Morphologies and stellar properties of gas-rich galaxies
Authors: Karki Arjun , Kulkarni Varsha P , Weng Simon , Péroux Céline , Augustin Ramona , Hayes Matthew , Ayromlou Mohammadreza , Kacprzak Glenn G , Howk J Christopher , Szakacs Roland , Klitsch Anne , Hamanowicz Aleksandra , Fresco Alejandra , Zwaan Martin A , Biggs Andrew D , Fox Andrew J , Kassin Susan , Kuntschner Harald 
Published: August 2023

ABSTRACT
Understanding how galaxies interact with the circumgalactic medium (CGM) requires determining how galaxies’ morphological and stellar properties correlate with their CGM properties. We report an analysis of 66 well-imaged galaxies detected in Hubble Space Telescope and Very Large Telescope MUSE observations and determined to be within ±500 km s−1 of the redshifts of strong intervening quasar absorbers at 0.2 ≲ z ≲ 1.4 with H i column densities $N_{\rm H I} \gt 10^{18}\, \rm cm^{-2}$. We present the geometrical properties (Sérsic indices, effective radii, axis ratios, and position angles) of these galaxies determined using galfit. Using these properties along with star formation rates (SFRs, estimated using the H α or [O ii] luminosity) and stellar masses (M* estimated from spectral energy distribution fits), we examine correlations among various stellar and CGM properties. Our main findings are as follows: (1) SFR correlates well with M*, and most absorption-selected galaxies are consistent with the star formation main sequence of the global population. (2) More massive absorber counterparts are more centrally concentrated and are larger in size. (3) Galaxy sizes and normalized impact parameters correlate negatively with NHI, consistent with higher NHI absorption arising in smaller galaxies, and closer to galaxy centres. (4) Absorption and emission metallicities correlate with M* and specific SFR, implying metal-poor absorbers arise in galaxies with low past star formation and faster current gas consumption rates. (5) SFR surface densities of absorption-selected galaxies are higher than predicted by the Kennicutt–Schmidt relation for local galaxies, suggesting a higher star formation efficiency in the absorption-selected galaxies.
The formation pathways of compact elliptical galaxies
Authors: Deeley Simon , Drinkwater Michael J , Sweet Sarah M , Bekki Kenji , Couch Warrick J , Forbes Duncan A 
Published: August 2023

ABSTRACT
Compact elliptical (cE) galaxies remain an elusively difficult galaxy class to study. Recent observations have suggested that isolated and host-associated cEs have different formation pathways, while simulation studies have also shown different pathways can lead to a cE galaxy. However, a solid link has not been established, and the relative contributions of each pathway in a cosmological context remains unknown. Here, we combine a spatially resolved observational sample of cEs taken from the Sydney-AAO Multi-object Integral field spectrograph Galaxy Survey with a matched sample of galaxies within the IllustrisTNG cosmological simulation to establish an overall picture of how these galaxies form. The observed cEs located near a host galaxy appear redder, smaller, and older than isolated cEs, supporting previous evidence for multiple formation pathways. Tracing the simulated cEs back through time, we find two main formation pathways; 32 ± 5 per cent formed via the stripping of a spiral galaxy by a larger host galaxy, while 68 ± 4 per cent formed through a gradual build-up of stellar mass in isolated environments. We confirm that cEs in different environments do indeed form via different pathways, with all isolated cEs in our sample having formed via in situ formation (i.e. none were ejected from a previous host), and 77 ± 6 per cent of host-associated cEs having formed via tidal stripping. Separating them by their formation pathway, we are able to reproduce the observed differences between isolated and host-associated cEs, showing that these differences can be fully explained by the different formation pathways dominating in each environment.