bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidimensional computational study to understand non-coding RNA interactions in breast cancer metastasis
Authors: Chakraborty Sohini , Banerjee Satarupa 
Published: September 2023

AbstractMetastasis is a major breast cancer hallmark due to which tumor cells tend to relocate to regional or distant organs from their organ of origin. This study is aimed to decipher the interaction among 113 differentially expressed genes, interacting non-coding RNAs and drugs (614 miRNAs, 220 lncRNAs and 3241 interacting drugs) associated with metastasis in breast cancer. For an extensive understanding of genetic interactions in the diseased state, a backbone gene co-expression network was constructed. Further, the mRNA–miRNA–lncRNA–drug interaction network was constructed to identify the top hub RNAs, significant cliques and topological parameters associated with differentially expressed genes. Then, the mRNAs from the top two subnetworks constructed are considered for transcription factor (TF) analysis. 39 interacting miRNAs and 1641 corresponding TFs for the eight mRNAs from the subnetworks are also utilized to construct an mRNA–miRNA–TF interaction network. TF analysis revealed two TFs (EST1 and SP1) from the cliques to be significant. TCGA expression analysis of miRNAs and lncRNAs as well as subclass-based and promoter methylation-based expression, oncoprint and survival analysis of the mRNAs are also done. Finally, functional enrichment of mRNAs is also performed. Significant cliques identified in the study can be utilized for identification of newer therapeutic interventions for breast cancer. This work will also help to gain a deeper insight into the complicated molecular intricacies to reveal the potential biomarkers involved with breast cancer progression in future.
Impact of red blood cell distribution width–albumin ratio on prognosis of patients with CKD
Authors: Kimura Hiroshi , Tanaka Kenichi , Saito Hirotaka , Iwasaki Tsuyoshi , Kazama Sakumi , Shimabukuro Michio , Asahi Koichi , Watanabe Tsuyoshi , Kazama Junichiro James 
Published: September 2023

AbstractThe red blood cell distribution width–albumin ratio (RAR) is a prognostic factor for adverse outcomes in various populations. However, whether RAR is associated with renal outcomes remains unclear. Therefore, we aimed to investigate the impact of RAR on the prognosis in patients with chronic kidney disease (CKD). We conducted a retrospective cohort study using 997 CKD patients who were enrolled in the Fukushima Cohort Study. Patients were categorized into tertiles (T1-3) according to the baseline RAR. The associations of RAR with end-stage kidney disease (ESKD) were assessed using Kaplan–Meier curves and multivariable cox regression analyses. Receiver operating characteristic (ROC) curves were performed to test whether significant differences were present between red cell distribution width (RDW) and RAR. The median age was 66, 57% were men, the median eGFR was 47.8 ml/min/1.73 m2, and the median value of RAR was 3.5. The higher RAR group showed an increased risk for ESKD in the Kaplan–Meier curve analysis. Compared to the lowest RAR group, higher RAR groups had a higher risk of ESKD (hazard ratio [HR] 1.37, 95% CI 0.68–2.78 and 2.92, 95% CI 1.44–5.94) for T2 and T3 groups, respectively. ROC curve analysis proved that the discriminating ability of RAR for ESKD was superior to RDW. A higher RAR value was associated with worse renal outcomes in patients with CKD. RAR could be a convenient and useful prognostic marker for renal prognosis.
SL-scan identifies synthetic lethal interactions in cancer using metabolic networks
Authors: Zangene Ehsan , Marashi Sayed-Amir , Montazeri Hesam 
Published: September 2023

AbstractExploiting synthetic lethality is a promising strategy for developing targeted cancer therapies. However, identifying clinically significant synthetic lethal (SL) interactions among a large number of gene combinations is a challenging computational task. In this study, we developed the SL-scan pipeline based on metabolic network modeling to discover SL interaction. The SL-scan pipeline identifies the association between simulated Flux Balance Analysis knockout scores and mutation data across cancer cell lines and predicts putative SL interactions. We assessed the concordance of the SL pairs predicted by SL-scan with those of obtained from analysis of the CRISPR, shRNA, and PRISM datasets. Our results demonstrate that the SL-scan pipeline outperformed existing SL prediction approaches based on metabolic networks in identifying SL pairs in various cancers. This study emphasizes the importance of integrating multiple data sources, particularly mutation data, when identifying SL pairs for targeted cancer therapies. The findings of this study may lead to the development of novel targeted cancer therapies.
Drug-induced social connection: both MDMA and methamphetamine increase feelings of connectedness during controlled dyadic conversations
Authors: Molla Hanna , Lee Royce , Lyubomirsky Sonja , de Wit Harriet 
Published: September 2023

AbstractMDMA is a stimulant-like drug with distinctive empathogenic effects. Its pro-social effects, such as feelings of connectedness, may contribute to both its popularity as a recreational drug and its apparent value as an adjunct to psychotherapy. However, little is known about the behavioral processes by which MDMA affects social interactions. This investigation examined the effects of MDMA (100 mg versus placebo; N = 18) on feelings of connectedness with an unfamiliar partner during a semi-structured casual conversation. A separate study examined the effects of a prototypic stimulant methamphetamine (MA; 20 mg versus placebo; N = 19) to determine the pharmacological specificity of effects. Oxytocin levels were obtained in both studies. Compared to placebo, both MDMA and MA increased feelings of connection with the conversation partners. Both MDMA and MA increased oxytocin levels, but oxytocin levels were correlated with feeling closer to the partner only after MDMA. These findings demonstrate an important new dimension of the pro-social effects of MDMA, its ability to increase feelings of connectedness during casual conversations between two individuals. Surprisingly, MA had a similar effect. The findings extend our knowledge of the social effects of these drugs, and illustrate a sensitive method for assessing pro-social effects during in-person dyadic encounters.
ANGPTL2 promotes immune checkpoint inhibitor-related murine autoimmune myocarditis
Authors: Horiguchi Haruki , Kadomatsu Tsuyoshi , Yamashita Tomoya , Yumoto Shinsei , Terada Kazutoyo , Sato Michio , Morinaga Jun , Miyata Keishi , Oike Yuichi 
Published: September 2023

AbstractUse of immune checkpoint inhibitors (ICIs) as cancer immunotherapy advances rapidly in the clinic. Despite their therapeutic benefits, ICIs can cause clinically significant immune-related adverse events (irAEs), including myocarditis. However, the cellular and molecular mechanisms regulating irAE remain unclear. Here, we investigate the function of Angiopoietin-like protein 2 (ANGPTL2), a potential inflammatory mediator, in a mouse model of ICI-related autoimmune myocarditis. ANGPTL2 deficiency attenuates autoimmune inflammation in these mice, an outcome associated with decreased numbers of T cells and macrophages. We also show that cardiac fibroblasts express abundant ANGPTL2. Importantly, cardiac myofibroblast-derived ANGPTL2 enhances expression of chemoattractants via the NF-κB pathway, accelerating T cell recruitment into heart tissues. Our findings suggest an immunostimulatory function for ANGPTL2 in the context of ICI-related autoimmune inflammation and highlight the pathophysiological significance of ANGPTL2-mediated cardiac myofibroblast/immune cell crosstalk in enhancing autoimmune responses. These findings overall provide insight into mechanisms regulating irAEs.
Intrinsically disordered regions in TRPV2 mediate protein-protein interactions
Authors: Sanganna Gari Raghavendar R. , Tagiltsev Grigory , Pumroy Ruth A. , Jiang Yining , Blackledge Martin , Moiseenkova-Bell Vera Y. , Scheuring Simon 
Published: September 2023

AbstractTransient receptor potential (TRP) ion channels are gated by diverse intra- and extracellular stimuli leading to cation inflow (Na+, Ca2+) regulating many cellular processes and initiating organismic somatosensation. Structures of most TRP channels have been solved. However, structural and sequence analysis showed that ~30% of the TRP channel sequences, mainly the N- and C-termini, are intrinsically disordered regions (IDRs). Unfortunately, very little is known about IDR ‘structure’, dynamics and function, though it has been shown that they are essential for native channel function. Here, we imaged TRPV2 channels in membranes using high-speed atomic force microscopy (HS-AFM). The dynamic single molecule imaging capability of HS-AFM allowed us to visualize IDRs and revealed that N-terminal IDRs were involved in intermolecular interactions. Our work provides evidence about the ‘structure’ of the TRPV2 IDRs, and that the IDRs may mediate protein-protein interactions.
Abnormal developmental trajectory and vulnerability to cardiac arrhythmias in tetralogy of Fallot with DiGeorge syndrome
Authors: Chan Chun-Ho , Lam Yin-Yu , Wong Nicodemus , Geng Lin , Zhang Jilin , Ahola Virpi , Zare Aman , Li Ronald Adolphus , Lanner Fredrik , Keung Wendy , Cheung Yiu-Fai 
Published: September 2023

AbstractTetralogy of Fallot (TOF) is the most common cyanotic congenital heart disease. Ventricular dysfunction and cardiac arrhythmias are well-documented complications in patients with repaired TOF. Whether intrinsic abnormalities exist in TOF cardiomyocytes is unknown. We establish human induced pluripotent stem cells (hiPSCs) from TOF patients with and without DiGeorge (DG) syndrome, the latter being the most commonly associated syndromal association of TOF. TOF-DG hiPSC-derived cardiomyocytes (hiPSC-CMs) show impaired ventricular specification, downregulated cardiac gene expression and upregulated neural gene expression. Transcriptomic profiling of the in vitro cardiac progenitors reveals early bifurcation, as marked by ectopic RGS13 expression, in the trajectory of TOF-DG-hiPSC cardiac differentiation. Functional assessments further reveal increased arrhythmogenicity in TOF-DG-hiPSC-CMs. These findings are found only in the TOF-DG but not TOF-with no DG (ND) patient-derived hiPSC-CMs and cardiac progenitors (CPs), which have implications on the worse clinical outcomes of TOF-DG patients.
A discounted‐UCB1‐tuned plus Q‐learning based anti‐jamming routing algorithm for wireless mesh networks
Authors: Ding Huihui , Zhou Quan , Niu Yingtao , Xiang Peng , Zhou Zhanyang 
Published: September 2023

AbstractTo achieve autonomous anti‐jamming routing for wireless mesh networks (WMNs) under the threat of unknown removable jammers, a Q‐learning anti‐jamming routing algorithm using the improved upper‐confidence‐bound (UCB) algorithm is proposed in this paper. Specifically, the WMNs anti‐jamming routing path finding problem is first modelled as a Markov decision process. Then, to address the slow‐convergence anti‐jamming strategy induced by the drawback that the preset parameters uniquely determine the length of the traditional Q‐learning exploration process, a discounted‐UCB‐tuned algorithm (Dis‐UCB1‐tuned) is adopted as the decision algorithm to replace the ε‐greedy or softmax algorithm in traditional Q‐learning. Finally, the simulation results demonstrate the superiority of the proposed anti‐jamming scheme over the UCB‐based Q learning(UCBQ) algorithm, Dyna‐Q algorithm, and the traditional Q‐learning algorithm. To elaborate, the proposed scheme can fast converge to a superior anti‐jamming routing without any prior knowledge of jamming environment, and its performance is also independent with the preset parameter.
Ag‐doped Ti3C2Tx sensor: A promising candidate for low‐concentration H2S gas sensing
Authors: Zeng Fuping , Feng Xiaoxuan , Chen Xiaoyue , Qiu Hao , Yan Yiming , Yao Qiang , Tang Ju 
Published: September 2023

AbstractTrace hydrogen sulphide (H2S) could reflect the severity of insulation faults in gas‐insulated switchgear (GIS), therefore, accurate and fast detection of low‐concentration H2S is important for on‐line monitoring, fault diagnosis, and state evaluation in GIS. Ag‐Ti3C2Tx chemiresistive‐type sensors were fabricated via drop‐coating with self‐reduction synthesised Ag‐doped Ti3C2Tx. The as‐prepared sensors exhibited an excellent sensitivity and selectivity to H2S with an extremely low detection of limit of 18.57 parts per billion (ppb) at 25°C (room temperature). The response of Ag‐Ti3C2Tx sensor to 10 parts per million (ppm) H2S was enhanced ∼12 times than that of the pristine Ti3C2Tx sensor. The compositing of Ti3C2Tx with Ag nanoparticles (NPs) enabled the fast response/recovery time for H2S detection. Further analysis found that the enhanced H2S sensing performances could be attributed to chemical sensitisation, adsorbed oxygen species regulation and high Brunauer–Emmett–Teller (BET) surface area. This study paves the way for Ag‐Ti3C2Tx as room‐temperature sensing materials to detect low‐concentration H2S in GIS.
A heliospheric density and magnetic field model
Authors: Mann G. , Warmuth A. , Vocks C. , Rouillard A. P. 
Published: September 2023

Context. The radial evolution of the density of the plasma and the magnetic field in the heliosphere, especially in the region between the solar corona and the Earth’s orbit, has been a topic of active research for several decades. Both remote-sensing observations and in situ measurements by spacecraft such as HELIOS, Ulysses, and WIND have provided critical data on this subject. The NASA space mission Parker Solar Probe (PSP), which will approach the Sun down to a distance of 9.9 solar radii on December 24, 2024, gives new insights into the structure of the plasma density and magnetic field in the heliosphere, especially in the near-Sun interplanetary space. This region is of particular interest because the launch and evolution of coronal mass ejections (CMEs), which can influence the environment of our Earth (usually called space weather), takes place there.
Aims. Because of the new data from PSP, it is time to revisit the subject of the radial evolution of the plasma density and magnetic field in the heliosphere. To do this, we derive a radial heliospheric density and magnetic field model in the vicinity of the ecliptic plane above quiet equatorial regions. The model agrees well with the measurements in the sense of a global long-term average.
Methods. The radial evolution of the density and solar wind velocity is described in terms of Parker’s wind equation. A special solution of this equation includes two integration constants that are fitted by the measurements. For the magnetic field, we employed a previous model in which the magnetic field is describe by a superposition of the magnetic fields of a dipole and a quadrupole of the quiet Sun and a current sheet in the heliosphere.
Results. We find the radial evolution of the electron and proton number density as well as the radial component of the magnetic field and the total field strength in the heliosphere from the bottom of the corona up to a heliocentric distance of 250 solar radii. The modelled values are consistent with coronal observations, measurements at 1 AU, and with the recent data from the inner heliosphere provided by PSP.
Conclusions. With the knowledge of the radial evolution of the plasma density and the magnetic field in the heliosphere the radial behaviour of the local Alfvén speed can be calculated. It can can reach a local maximum of 392 km s−1 at a distance of approximately 4 solar radii, and it exceeds the local solar wind speed at distances in the range of 3.6−13.7 solar radii from the centre of the Sun.
Vergleich der Lebensqualität nach operativer Therapie einer vertebralen Osteomyelitis und degenerativer Spondylolisthesis
Authors: Beyer Frank , Yagdiran Ayla , Eysel Peer , Bredow Jan 
Published: September 2023

ZusammenfassungDie vertebrale Osteomyelitis (VO) und die degenerative Spondylolisthesis (SL) sind 2 häufig behandelte Krankheitsbilder an der Wirbelsäule. In der vorgelegten Arbeit wird daher die
Lebensqualität nach operativer Therapie dieser beiden Entitäten anhand etablierter Scores verglichen.In einer monozentrischen Untersuchung wurden alle Patienten mit einer vertebralen Osteomyelitis und degenerativer Spondylolisthesen mithilfe des Spine-Tango-Registers prospektiv erfasst.
Chirurgisch erfolgte die ein- oder zweizeitige Fusion der betroffenen Segmente. Die Lebensqualität wurde mit dem Core Outcome Measures Index (COMI) und dem Oswestry Disability Index (ODI) zu
den Zeitpunkten t0 (0 Monate), t1 (12 Monate) und t2 (24 Monate) erhoben. Die statistische Analyse erfolgte mit SPSS 28.0. Das Signifikanzniveau wurde auf 5% festgesetzt.52 Patienten mit VO und 48 Patienten mit SL wurden in die Analyse eingeschlossen. Bei der Alters- und Geschlechtsverteilung zeigten sich keine signifikanten Unterschiede. Die Verweildauer
in der SL-Gruppe war signifikant kürzer (p < 0,001). Der ODI zum Zeitpunkt t0 war in der VO-Gruppe signifikant höher (p < 0,001), hingegen unterschieden sich die COMI-Scores nicht
signifikant (p = 0,155). Zu den Zeitpunkten t1 und t2 waren die Unterschiede zwischen der VO- und SL-Gruppe weder für den ODI-Score signifikant verschieden (p = 0,176; p = 0,250) noch für den
COMI-Score (p = 0,682; p = 0,640).Die postoperative Lebensqualität nach lumbaler Fusionsoperation bei SL und VO sind bei unterschiedlicher Indikation und Ausgangslage vergleichbar. In beiden Gruppen wird ein ODI- und
COMI-Score chronischer Rückenschmerzpatienten erreicht. Für die präoperativ bestehende Erwartungshaltung an eine Operation sollte dies ebenso wie für die Indikationsstellung bedacht
werden.
Drug-Resistant Strains in Surgical Site Infections after Abdominal Surgery: A Prospective Study
Authors: Gupta Veenu , Kaur Prateet , Chhina Deepinder , Singh Jaspal , Gupta Rama 
Published: September 2023

Background Surgical site infection (SSI) is a common complication of abdominal surgery associated with substantial discomfort, morbidity, and cost.
Objective The aim of this study was to determine the incidence, risk factors, and antimicrobial susceptibility pattern of the associated bacterial causes of SSI in patients after abdominal surgery.
Methods A prospective study over a 1-year (Jan 2019–Dec 2019) period was conducted at a tertiary care institution in North India. Postoperative patients of abdominal surgeries with SSI were included in the study. Any exudate from the surgical site was collected aseptically and was processed as per standard operating procedures.
Results Of 2,509 patients with abdominal surgeries included in the study, 75 (2.98%, majority, i.e., 50 males) developed SSI. Common risk factors found to be associated with the development of SSI include contaminated surgical sites, obesity, age, immunosuppression, and simultaneous infection at some other sites, with a predominance (89.3%) of gram-negative isolates. Escherichia coli was the most frequently isolated organism (45.3%), followed by Klebsiella spp. (20%), Acinetobacter spp.(10.6%), Pseudomonas spp., and Staphylococcus aureus (8% each). There was a very high (53%) prevalence of extended-spectrum β lactamase production among the E. coli and Klebsiella isolates. Additionally, 58.8% of the E. coli isolates were multidrug-resistant, whereas 73.3% of the Klebsiella isolates were exclusively drug resistant. All the S. aureus isolates were found to be methicillin-resistant.
Conclusion Although the SSI rates after abdominal surgery were low, multidrug-resistant gram-negative bacteria were predominant in SSI.
Creative practice as research in Old Norse-Icelandic studies: Ancillary characters as storytellers
Authors: Gíslason Kári , Bennett Lisa 
Published: September 2023

AbstractThis article is a discussion between two writer-academics about projects that re-imagined medieval Icelandic sagas from the perspectives of female characters in these works, and in ways that adopted conventions of interiority and point of view associated with modern creative writing. The discussion examines the potential for creative practice to form a research methodology within Old Norse-Icelandic studies. In particular, the contingent or open-ended nature of creative practice makes it a vehicle by which to raise new questions in relation to texts that have been the subject of extensive prior study. While creative practice as research is to some extent limited by its personal and often quite individual nature, it does offer methods by which imagination and the deeply engaged act of making and re-telling can form part of our understanding of Old Norse-Icelandic texts.
The impact of education and digitalization on female labour force participation in BRICS: an advanced panel data analysis
Authors: Shuangshuang Yu , Zhu Wenzhong , Mughal Nafeesa , Aparcana Sergio Ivan Vargas , Muda Iskandar 
Published: September 2023

AbstractThe present study empirically elucidates the interconnections between digitalization and FLFP in BRICS economies. Furthermore, we also assessed the influence of three essential economic indicators on FLFP, i.e., education, fertility and GDP. We used annual data from 1990 to 2020 and advanced panel estimation techniques such as Banerjee and Crrion-i-Selvestre cointegration, slope heterogeneity, and cross-sectional augmented autoregressive distributed lags model to effectuate these objectives. The study’s empirical results illustrate the existence of a positive interconnection between digitalization and FLFP in sample countries. In addition, education and GDP play an essential role in furthering FLFP in the long run. A similar interconnection is observed in the short period. However, the magnitude of the short-run impacts is smaller than the long-run impacts. The empirical analysis offers a few pertinent policy insights for policymakers to improve the situation in the selected sample.
Design and fabrication of a low-cost microfluidic cartridge with integrated pressure-driven check valve for molecular diagnostics platforms
Authors: Downen R Scott , Dong Quan , Chen Julius Lee , Li Zhenyu 
Published: September 2023

Abstract
This paper describes the design, fabrication, and preliminary testing of a low-cost, easy to manufacture microfluidics cartridge capable of fluid storage and manipulation through a custom pressure-driven check valve. Cartridge components are fabricated using a desktop CNC and laser cutter, the check valve is fabricated using PDMS in a custom acrylic mold, and the components are assembled using a thermal diffusion welder. Following assembly, preliminary testing of the cartridge, including fluid manipulation and use for molecular diagnostics, was performed. To pull a sample into the lysing chamber, a vacuum over 1.4PSI was required. No opening of the valve to the reaction chamber was observed. Moving fluid across the custom valve from the lysing chamber to the reaction chamber then required a vacuum over 4.5PSI. Finally, a proof-of-concept demonstration of one potential application was performed using a custom benchtop LAMP system for molecular diagnostic testing. The low-cost nature of the design, ease of manufacturing, fluid storage and manipulation demonstrated make this design ideal for research and high-volume testing in low resource environments.