bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0084188 promotes colorectal cancer progression by sponging miR‐654‐3p and regulating kruppel‐like factor 12
Authors: Wu Cui‐Cui , Hou Bai‐Chun , Yang Yu‐Han , Li Xue‐Feng , Ma Hong‐Chao , Li Bin‐Xian 
Published: September 2023

AbstractTo investigate the biological role and mechanism of circ_0084188 in colorectal cancer (CRC). Real‐time quantitative polymerase chain reaction and western blot assay were used to detect RNA levels and protein levels in CRC cell lines (HCT116 and SW480), respectively. Cell proliferation was evaluated by Cell Counting Kit‐8 assay, 5‐ethynyl‐2′‐deoxyuridine assay, and colony formation assays. Cell apoptosis was determined using flow cytometry. Cell migration and invasion were measured by transwell assay. Sphere formation efficiency was determined by sphere formation assay. The interaction between microRNA‐654‐3p (miR‐654‐3p) and circ_0084188 or Kruppel‐like factor 12 (KLF12) was confirmed by a dual‐luciferase reporter, RNA immunoprecipitation and RNA pull‐down assays. Xenograft in CRC mice model was utilized for exploring the role of circ_0084188 in vivo.Circ_0084188 was overexpressed in CRC tissues and cells. Circ_0084188 silencing suppressed cell proliferation, migration, invasion, and stemness and induced apoptosis in CRC cells. Circ_0084188 acted as a sponge for miR‐654‐3p, and circ_0084188 regulated CRC cell behaviors via sponging miR‐654‐3p. Moreover, KLF12 was a target of miR‐654‐3p, and miR‐654‐3p overexpression inhibited the malignant behaviors of CRC cells by downregulating KLF12. Mechanically, circ_0084188 sponged miR‐654‐3p to regulate KLF12 expression in CRC cells. In addition, circ_0084188 downregulation inhibited tumor growth in vivo.Circ_0084188 knockdown might repress CRC progression partially via regulating the miR‐654‐3p/KLF12 axis, providing a novel insight into the pathogenesis of CRC.
Longitudinal Performance of Cochlear Implants in Neurofibromatosis Type 2
Authors: Seo Stefanie , Shen Sarek , Ding Andy S. , Creighton Francis X. 
Published: September 2023

ObjectiveCochlear implants (CIs) are a well‐established treatment modality for hearing loss due to neurofibromatosis type 2 (NF2). Our aim is to investigate variables that affect longitudinal performance of CIs among patients with NF2.Study DesignRetrospective review at a single academic institution consisting of patients who have received cochlear implants following hearing loss due to NF2.MethodsThe primary outcome examined was CI disuse or explantation. Associated clinical and surgical variables were analyzed using descriptive statistics. These included postoperative pure tone average (PTA) at 500, 1000, and 2000 Hz, tumor size, previous surgery, and comorbid depression.ResultsA total of 12 patients and 14 cochlear implants received at our institution from 2001 to 2022 were included. Notably, 35.7% of CIs (5 out of 14 cases) resulted in disuse or explantation. The average interval until explant was 9.4 years (range 3–14 years). In explanted CI cases, 20% had previous surgery and 80% had a diagnosis of comorbid depression as compared to 22.2% and 22.2%, respectively, in intact CI cases. Maximum tumor diameter was the only variable found to impact CI usage outcome (p = 0.028). Long‐term data showed that on average, patients benefit from 13.85 years of CI utility and a maximum PTA improvement of 45.0 ± 29.0 dB.ConclusionDespite the recurrent nature of NF2, patients continue to receive audiological benefit from cochlear implants. We found that larger tumor size may be associated with longitudinal CI failure.Level of Evidence4 Laryngoscope, 134:1847–1853, 2024
Treatment Outcome in Head and Neck Cancer With Distant Metastasis at Initial Diagnosis
Authors: Omata Jo , Ueki Yushi , Takahashi Yuto , Tanaka Ryoko , Yokoyama Yusuke , Takahashi Takeshi , Shodo Ryusuke , Yamazaki Keisuke , Togashi Takafumi , Matsuyama Hiroshi , Takahashi Nao , Okabe Ryuichi , Horii Arata 
Published: September 2023

ObjectiveRecurrent/metastatic head and neck squamous cell carcinoma (HNSCC) treatment has changed dramatically with the introduction of immune checkpoint inhibitors (ICIs). However, there are few reports of treatment outcomes on HNSCC with distant metastasis (M1) at initial diagnosis, and its treatment strategy has not been standardized. We aimed to analyze the treatment outcome and prognostic factors of patients with HNSCC with initial M1 disease.MethodsIn this multi‐institutional retrospective study, 98 patients with HNSCC were initially diagnosed with M1 disease between 2007 and 2021. The patients were divided into the non‐palliative (received any systemic chemotherapy, n = 60) and palliative (did not receive systemic chemotherapy, n = 38) groups. Overall survival (OS) was compared between the groups. In the non‐palliative group, predictors of OS were explored based on patient characteristics and treatment details.ResultsThe median OS in the non‐palliative group was 15 months (95% confidence interval [CI], 10–20), which was significantly longer than that in the palliative group (3 months, 95% CI, 2–5) (p < 0.001). Multivariate analysis revealed that administration of locoregional radiation therapy (RT) (hazard ratio [HR] 0.407 [95% CI 0.197–0.844]; p = 0.016), ICIs (HR 0.216 [95% CI 0.088–0.532]; p < 0.001) and RT/surgery for distant metastasis (HR 0.373 [95% CI 0.150–0.932]; p = 0.034) were the independent prognostic factors of OS.ConclusionAn intensive treatment strategy combining systemic therapy using ICIs with RT/surgery for locoregional or distant metastasis may yield a survival benefit for patients with HNSCC with M1 disease.Level of Evidence4 Laryngoscope, 134:1679–1686, 2024
A jurisdictional risk assessment for the whole community: A new, systematic approach to participatory decision‐making in public health emergency preparedness using the analytic hierarchy process
Authors: Ray Madhury , Tornello Alana R. , Pickart Françoise , Stripling Mitch , Ali Mustafa , Vargas Luis G. 
Published: September 2023

AbstractIn 2018, the New York City (NYC) Department of Health and Mental Hygiene piloted a novel jurisdictional risk assessment (JRA) for public health disasters using the analytic hierarchy process (2018 JRA‐AHP). This new approach offers a replicable and equitable model for consensus decision‐making that incorporates the complexities of disaster preparedness. Its main contribution to the field is to offer a practical manifestation of emergency management's Whole Community Approach. The 2018 JRA‐AHP applied this principle by expanding traditional definitions of ‘expertise’ to include lived experience of disaster and facilitating direct participation in the risk decision by a variety of individuals with diverse backgrounds, expertise and knowledge of public health disasters. This paper describes the theory, methods and results behind the JRA‐AHP. The paper also presents a critical analysis of public health disaster risk assessments; contextualizes the Whole Community Approach using models of the relationship between democratic governments and communities; and addresses the practical applications of the 2018 JRA‐AHP in NYC while exploring the potential challenges that other jurisdictions may face in attempted adaptation.
The impact of (simulated) resolution on breast cancer diagnosis based on high‐resolution 3D micro‐CT microcalcification images
Authors: Brahimetaj Redona , Cornelis Jan , Van Ongeval Chantal , De Mey Johan , Jansen Bart 
Published: September 2023

AbstractBackgroundBreast microcalcifications (MCs) are considered to be a robust marker of breast cancer. A machine learning model can provide breast cancer diagnosis based on properties of individual MCs ‐ if their characteristics are captured at high resolution and in 3D.PurposeThe main purpose of the study was to explore the impact of image resolution (8 µm, 16 µm, 32 µm, 64 µm) when diagnosing breast cancer using radiomics features extracted from individual high resolution 3D micro‐CT MC images.MethodsBreast MCs extracted from 86 female patients were analyzed at four different spatial resolutions: 8 µm (original resolution) and 16 µm, 32 µm, 64 µm (simulated image resolutions). Radiomic features were extracted at each image resolution in an attempt, to find a compact feature signature allowing to distinguish benign and malignant MCs. Machine learning algorithms were used for classifying individual MCs and samples (i.e., patients). For sample diagnosis, a custom‐based thresholding approach was used to combine individual MC results into sample results. We conducted classification experiments when using (a) the same MCs visible in 8 µm, 16 µm, 32 µm, and 64 µm resolution; (b) the same MCs visible in 8 µm, 16 µm, and 32 µm resolution; (c) the same MCs visible in 8 µm and 16 µm resolution; (d) all MCs visible in 8 µm, 16 µm, 32 µm, and 64 µm resolution. Accuracy, sensitivity, specificity, AUC, and F1 score were computed for each experiment.ResultsThe individual MC results yielded an accuracy of 77.27%, AUC of 83.83%, F1 score of 77.25%, sensitivity of 80.86%, and specificity of 72.2% at 8 µm resolution. For the individual MC classifications we report for the F1 scores: a 2.29% drop when using 16 µm instead of 8 µm, a 4.01% drop when using 32 µm instead of 8 µm, a 10.69% drop when using 64 µm instead of 8 µm. The sample results yielded an accuracy and F1 score of 81.4%, sensitivity of 80.43%, and specificity value of 82.5% at 8 µm. For the sample classifications we report for F1 score values: a 6.3% drop when using 16 µm instead of 8 µm, a 4.91% drop when using 32 µm instead of 8 µm, and a 6.3% drop when using 64 µm instead of 8 µm.ConclusionsThe highest classification results are obtained at the highest resolution (8 µm). If breast MCs characteristics could be visualized/captured in 3D at a higher resolution compared to what is used nowadays in digital mammograms (approximately 70 µm), breast cancer diagnosis will be improved.
Heme oxygenase‐1 therapy attenuates muscle atrophy following global brachial plexus avulsion in juvenile rats
Authors: Qin Hongjiu , Rui Jing , Lao Jie 
Published: September 2023

AbstractIntroduction/AimsBrachial plexus injury can seriously affect distal target muscle function, and long‐term denervation leads to irreversible structural damage. In the present study, we examined the effect of hemin, a heme oxygenase‐1 (HO‐1) inducer, on intrinsic forepaw muscle atrophy induced by pan‐plexus injury in juvenile rats, as well as its underlying mechanism.MethodsA global brachial plexus avulsion (GBPA) model of rat was established, and thirty 6‐wk‐old male rats were randomly divided into five groups: control, GBPA plus scramble small intering RNA (siRNA), GBPA plus scramble siRNA plus hemin, GBPA plus HO‐1 siRNA, and GBPA plus HO‐1 siRNA plus hemin. Hemin (50 mg/kg) was administered intraperitoneally once daily and the siRNA (5 μg) was injected intramuscularly twice a week. Intrinsic forepaw muscles were used for analysis. Myofiber cross‐sectional area (CSA), capillary‐to‐fiber ratio (C/F), and fiber‐type composition were assessed. The levels of inflammatory factors, ubiquitin‐protein ligases, and autophagy‐related proteins were also measured.ResultsWe found that hemin treatment could effectively ameliorate denervated intrinsic forepaw muscle atrophy and suppress type I to II myofiber‐type conversion. Hemin treatment failed to prevent muscle capillary loss after denervation. The levels of inflammatory factors (tumor necrosis factor alpha [TNFα] and interleukin 6 [IL‐6]), ubiquitin‐protein ligases (MuRF‐1 and MAFbx), and autophagy‐related proteins (BNIP3 and LC3B‐II/I ratio) were increased by denervation and HO‐1 therapy attenuated the increment.DiscussionUpregulation of HO‐1 might potentially be an effective strategy to alleviate denervation‐related muscle atrophy and might be a promising adjunctive treatment to improve hand function in children with pan‐plexus injury.
Improved SSA‐RBF neural network‐based dynamic 3‐D trajectory tracking model predictive control of autonomous underwater vehicles with external disturbances
Authors: Bao Han , Zhu Haitao , Liu Di 
Published: September 2023

AbstractThis paper studies the three‐dimensional (3‐D) dynamic trajectory tracking control of an autonomous underwater vehicle (AUV). As AUV is a typical nonlinear system, each degree of freedom is strongly coupled, so the traditional control method based on the nominal model of AUV cannot guarantee the accuracy of the control system. To solve this problem, we first propose a prediction model based on a radial basis function neural network (RBF‐NN). The nonlinearity of AUV is learned and modeled offline by RBF‐NN based on previous data. This model can reflect the time sequence state and control variables of AUV. Secondly, to avoid the overfitting problem in network training based on the traditional gradient descent method, a new adaptive chaotic sparrow search algorithm (ACSSA) is proposed to optimize the network parameters, to improve the full approximation ability of RBF‐NN to nonlinear systems. To eliminate the steady‐state error caused by external interference during AUV trajectory tracking, a nonlinear optimizer is designed by updating the deviation of the NN model output layer. In each sampling period, the predictive control law is calculated online according to the deviation between the predicted value and the actual value. In addition, the stability analysis based on the Lyapunov method proves the asymptotic stability of the controller. Finally, the 3‐D dynamic trajectory tracking the performance of AUV under different external disturbances is verified by MATLAB/Simulink, and the results show that the proposed controller is more efficient and robust than the standard model predictive controller (MPC) controller and the standard NN model predictive controller (NNPC).
Identifying potential emerging invasive non‐native species from the freshwater pet trade
Authors: Dickey James W. E. , Liu Chunlong , Briski Elizabeta , Wolter Christian , Moesch Simon , Jeschke Jonathan M. 
Published: September 2023

Abstract

An increasingly globalised world has facilitated the movement of non‐native species (NNS) via the poorly regulated international pet trade. While focus is increasingly being placed on preventative action to combat invasive NNS—often cheaper and less difficult than the management of established populations—successful prevention requires controlling potential pathways and obtaining baseline knowledge of species' availability.

Here we performed an in‐depth analysis of the freshwater pet trade as one major vector of NNS, compiling its species inventory and deriving threats of NNS release and establishment in the wild. With Germany as our study region, we surveyed pet stores, websites and the country's largest online classified portal, eBay Kleinanzeigen, recording the taxa encountered. For each species, we determined the likelihood of release based on availability and price (cheaper and/or more readily available species have been shown to be of greater risk), and the likelihood of establishment based on ecological niche breadth and niche overlap with environmental conditions in Germany.

The survey revealed 669 species, of which 651 were non‐native to Germany. Looking at release likelihood, more readily available species in pet stores and on websites proved to be cheaper. For websites, there was a significant effect of occurrence status (i.e. released, not released, native) on price, with released and native species being significantly cheaper. Species previously released in Germany and elsewhere demonstrated greater niche breadths and greater niche overlaps between their source regions and Germany; and for species released in Germany, there was a significantly positive relationship between the magnitude of niche overlap and the number of documented occurrences.

Finally, we combined our release and establishment likelihood findings under ‘Release Risk’ metrics to highlight the species most worthy of prioritisation. We propose these metrics as proactive methods for screening species in the trade, which can inform future policy direction and intervention.

Read the free Plain Language Summary for this article on the Journal blog.
Biometric magnetic resonance imaging analysis of fetal brain development in Down syndrome
Authors: Kitano Rie , Madan Neel , Mikami Takahisa , Madankumar Rajeevi , Skotko Brian G. , Santoro Stephanie , Ralston Steven J. , Bianchi Diana W. , Tarui Tomo 
Published: September 2023

AbstractObjectivesTo assess brain development in living fetuses with Down syndrome (DS) by biometric measurements on fetal brain magnetic resonance images (MRI).MethodsWe scanned 10 MRIs of fetuses with confirmed trisomy 21 at birth and 12 control fetal MRIs without any detected anomalies. Fetal brain MRIs were analyzed using 14 fetal brain and skull biometric parameters. We compared measures between DS and controls in both raw MRIs and motion‐corrected and anterior‐posterior commissure‐aligned images.ResultsIn the reconstructed images, the measured values of the height of the cerebellar vermis (HV) and anteroposterior diameter of the cerebellar vermis (APDV) were significantly smaller, and the anteroposterior diameter of the fourth ventricle (APDF) was significantly larger in fetuses with DS than controls. In the raw MRIs, the measured values of the right lateral ventricle were significantly larger in fetuses with DS than in controls. Logistic regression analyses revealed that a new parameter, the cerebellar‐to‐fourth‐ventricle ratio (i.e., (APDV * Height of the vermis)/APDF), was significantly smaller in fetuses with DS than controls and was the most predictive to distinguish between fetuses with DS and controls.ConclusionsThe study revealed that fetuses with DS have smaller cerebellums and larger fourth ventricles compared to the controls.
Prenatal diagnosis of PORCN‐related developmental syndrome in a fetus: A novel phenotype
Authors: Kilby Mark D. , Castleman James , Allen Stephanie , Doyle Samantha , Williams Denise K. 
Published: September 2023

AbstractWe report a case of a female fetus born to an unrelated couple with a complex fetal phenotype of a pleural effusion, a cardiac malformation, and syndactyly of the toes. Prenatal exome sequencing identified a variant of uncertain significance in the PORCN gene that was upgraded to likely pathogenic following postnatal clinical examination. The phenotype described in cases with variants in the PORCN gene is often associated with findings that cannot be prospectively diagnosed by ultrasonography. This is the first report of a prenatal phenotype involving a fetal effusion associated with variants in the PORCN gene, with skeletal findings identified later in gestation on ultrasonography. The diagnosis was confirmed on neonatal examination.
Halomonas ventosae JPT10 promotes salt tolerance in foxtail millet (Setaria italica) by affecting the levels of multiple antioxidants and phytohormones
Authors: Xiao Shenghui , Wan Yiman , Zheng Yue , Wang Yongdong , Fan Jiayin , Xu Qian , Gao Zheng , Wu Changai 
Published: September 2023

AbstractPlant growth‐promoting bacterias (PGPBs) can increase crop output under normal and abiotic conditions. However, the mechanisms underlying the plant salt tolerance‐promoting role of PGPBs still remain largely unknown. In this study, we demonstrated that Halomonas ventosae JPT10 promoted the salt tolerance of both dicots and monocots. Physiological analysis revealed that JPT10 reduced reactive oxygen species accumulation by improving the antioxidant capability of foxtail millet seedlings. The metabolomic analysis of JPT10‐inoculated foxtail millet seedlings led to the identification of 438 diversely accumulated metabolites, including flavonoids, phenolic acids, lignans, coumarins, sugar, alkaloids, organic acids, and lipids, under salt stress. Exogenous apigenin and chlorogenic acid increased the salt tolerance of foxtail millet seedlings. Simultaneously, JPT10 led to greater amounts of abscisic acid (ABA), indole‐3‐acetic acid (IAA), salicylic acid (SA), and their derivatives but lower levels of 12‐oxo‐phytodienoic acid (OPDA), jasmonate (JA), and JA‐isoleucine (JA‐Ile) under salt stress. Exogenous JA, methyl‐JA, and OPDA intensified, whereas ibuprofen or phenitone, two inhibitors of JA and OPDA biosynthesis, partially reversed, the growth inhibition of foxtail millet seedlings caused by salt stress. Our results shed light on the response of foxtail millet seedlings to H. ventosae under salt stress and provide potential compounds to increase salt tolerance in foxtail millet and other crops.
Ultrasound‐assisted freeze‐drying strategy to enhance nanoparticle dispersion in aerogels: A case study on polyimide/silica nanocomposite aerogel
Authors: Xiao Hang , Zhao Xi , Lv Jiangbo , He Xian , Chen Menghao , Tan Wei , Yang Wenjie , Zeng Ke , Hu Jianghuai , Yang Gang 
Published: September 2023

AbstractThermal protection materials should possess light weight, excellent thermal insulation, flame retardant and hydrophobic properties. Aerogel are considered a promising thermal protection material due to its high porosity and low density. Among them, nano‐silica/polyimide composite aerogel has been intensively studied as they can solve the problem of fragility of nano‐silica aerogel while ensuring thermal insulation and flame retardant performance. However, the problems of nanoparticle agglomeration and the tedious preparation process remain unsolved. The ultrasound‐assisted freeze‐drying (UAFD) strategy presented in this paper avoids the tedious silica solution preparation process by hydrolyzing silica precursors to achieve good dispersion of silica. This strategy replaces the traditional approach of functionalized modification of silica nanoparticles to inhibit their agglomeration with a new method of nanoparticle dispersion by ultrasound and rapid freezing to lock the dispersed state. The UAFD strategy enables the rapid and efficient preparation of well‐dispersed nano‐silica/polyimide composite aerogels, and the hierarchical pore structure of the composite aerogels is imparted by this facile preparation strategy that cooperates with the co‐regulation capability of the tert‐butanol on the morphology of the ice template. The aerogels have substantially improved thermal insulation, flame retardant, and hydrophobic properties when compared with those prepared by conventional freeze‐drying methods.Highlights
Ultrasound‐assisted freeze‐drying (UAFD) enhances nanoparticles dispersion.
UAFD and tert‐butanol synergistically prepared hierarchically pore structure.
Thermal protection performance of aerogels prepared by UAFD is improved.