bluuebunny/crossref_metadata_2025_split huggingface dataset:
HuR inhibition reduces post-ischemic cardiac remodeling by dampening acute inflammatory gene expression and the innate immune response
Authors: Slone Samuel , Anthony Sarah R. , Green Lisa C. , Nieman Michelle L. , Alam Perwez , Wu Xiaoqing , Roy Sudeshna , Aube Jeffrey , Xu Liang , Lorenz John N. , Owens A. Phillip , Kanisicak Onur , Tranter Michael 
Published: January 2023

ABSTRACTMyocardial ischemia/reperfusion (I/R) injury and the resulting cardiac remodeling is a common cause of heart failure. The RNA binding protein Human Antigen R (HuR) has been previously shown to reduce cardiac remodeling following both I/R and cardiac pressure overload, but the full extent of the HuR-dependent mechanisms within cells of the myocardium have yet to be elucidated. In this study, we applied a novel small molecule inhibitor of HuR to define the functional role of HuR in the acute response to I/R injury and gain a better understanding of the HuR-dependent mechanisms during post-ischemic myocardial remodeling. Our results show an early (two hours post-I/R) increase in HuR activity that is necessary for early inflammatory gene expression by cardiomyocytes in response to I/R. Surprisingly, despite the reductions in early inflammatory gene expression at two hours post-I/R, HuR inhibition has no effect on initial infarct size at 24-hours post-I/R. However, in agreement with previously published work, we do see a reduction in pathological remodeling and preserved cardiac function at two weeks post-I/R upon HuR inhibition. RNA-sequencing analysis of neonatal rat ventricular myocytes (NRVMs) at two hours post-LPS treatment to model damage associated molecular pattern (DAMP)-mediated activation of toll like receptors (TLRs) demonstrates a broad HuR-dependent regulation of pro-inflammatory chemokine and cytokine gene expression in cardiomyocytes. We show that conditioned media from NRVMs pre-treated with HuR inhibitor loses the ability to induce inflammatory gene expression in bone marrow derived macrophages (BMDMs) compared to NRVMs treated with LPS alone. Functionally, HuR inhibition in NRVMs also reduces their ability to induce endocrine migration of peripheral blood monocytesin vitroand reduces post-ischemic macrophage infiltration to the heartin vivo.In summary, these results suggest a HuR-dependent expression of pro-inflammatory gene expression by cardiomyocytes that leads to subsequent monocyte recruitment and macrophage activation in the post-ischemic myocardium.
Association Between Primary Tumour–Brain Metastasis Receptor Status Mismatch and Outcomes in Breast Cancer Brain Metastasis
Authors: Zheng Yilong , Low Chen Ee , Tan Sheryl Yen Pin , Hing Clarisse Wei Yen , Yau Chun En , Teo Kejia , Nga Vincent Diong Weng , Yeo Tseng Tsai , Wong Andrea Li-Ann , Lim Mervyn Jun Rui 
Published: January 2023

Structured abstractObjectiveTo evaluate the association between primary tumour–brain metastasis receptor status mismatch and outcomes in patients with breast cancer brain metastasis (BCBM).MethodsPatients who (1) had a histologically verified breast-to-brain metastasis, (2) were 18 years old or above on the day of surgical resection, and (3) had the ER (estrogen receptor), PR (progesterone receptor), and HER2 (human epidermal growth factor receptor 2) statuses of both the primary breast tumour and the secondary brain metastasis were retrospectively recruited. Univariate time-to-event analysis was performed using the Kaplan-Meier method. The exposures analysed were the various combinations of ER, PR, and HER2 statuses between the primary breast tumour and the secondary brain metastasis. The outcomes were overall mortality and recurrence.ResultsOf the 158 patients who underwent surgical resection of brain metastases during the study period, 31 were included in the analysis. The mean (SD) age of the study population was 56.7 (12.2), and most patients were Chinese (54.8%). On univariate analysis of the association between the various receptor status combinations and overall mortality, ER (p=0.920) and PR (p=0.390) status conversion were both found to not be associated with overall mortality. However, HER2 status conversion was found to be significantly associated with overall mortality (p=0.026). Specifically, patients whose primary tumour was HER2+ but whose secondary brain metastasis was HER2− had the poorest outcome, with a median overall survival of 3.4 months. On the other hand, the median overall survival of the other HER2 receptor status combinations ranged from 10.9 to 16.6 months. There were no statistically significant associations between status conversion of any of the receptors and recurrence.ConclusionsAmong patients who underwent surgical resection of BCBMs, patients with primary tumour HER2+ but secondary brain metastasis HER2− had a significantly higher risk of mortality. However, ER and PR status conversion was not significantly associated with outcomes.
A Cost-effective Purification Process for Erythropoietin Biosimilar through Downstream Process Engineering
Authors: Nag Kakon , Haq Sarker Md. Enamul , Kumar Samir , Chakraborty Sourav , Khan Habiba , Islam Md. Jikrul , Rahman Khan Md. Maksusdur , Rahman Chowdhury Md. Mashfiqur , Roy Rony , Roy Ratan , Biswas Bipul Kumar , Hasan Bappi Md. Emrul , Mohiuddin Mohammad , Sultana Naznin 
Published: January 2023

AbstractWell-characterized and scalable downstream process for purification of biologics is extremely demanding for delivering quality therapeutics to patients at a reasonable price. Erythropoietin (EPO) is a blockbuster biologic with diverse clinical applications but its application is limited to financially well-off societies due to high price. The high price of EPO is associated with the technical difficulties related to the purification challenge to obtain qualified product with a cost-effective defined process. Though there are reports for purification of EPO but there is no report of well-characterized downstream process with critical process parameters (CPPs) that can deliver EPO consistently satisfying the quality target product profile (QTPP), which is a critical regulatory requirement. To advance the field, we applied quality by design (QbD) principle and design of experiment (DoE) protocol to establish an effective process, which is scalable up to 100× batch size satisfying QTPP. We have successfully transformed the process from static mode to dynamic mode and validated. Insignificant variation (p> 0.05) within and between 1×, 10× and 100× batches showed that the process is reproducible and seamlessly scalable. The biochemical analysis along with the biofunctionality data ensures that the products from different-scale batches were indifferent and comparable to a reference product. Our study thereby established a robust and scalable downstream process of EPO biosimilar satisfying QTPP. The technological scheme presented here can speed-up the production of not only EPO but many other life-saving biologics and make them available to mass population at a reduced cost.
Bacopa monnieriphytochemicals regulate fibroblast cell migration via modulation of focal adhesions
Authors: Zirmire Ravindra K. , Saha Dyuti , Tanimu Habibu , Zaarour Rania , Bird Deborah , Cherian Prakash , Misra Namita , Sanyal Aryasekhar , Roy Nita , Jamora Colin 
Published: January 2023

ABSTRACTTheBacopa monnieriplant contains a large repertoire of active phytochemicals that have been used extensively in traditional medicine for the treatment of various complex diseases. More recently it has been shown to increase the wound healing rate in rats, though its mechanism of action is largely unknown. Here we investigated the cellular pathways activated by a methanol extract ofBacopa monnieriin human dermal fibroblasts, which play many critical roles in the wound healing program. Gene expression analysis revealed thatBacopa monnieriextract can enhance tissue repair by modulating multiple processes involved in the wound healing program such as migration, proliferation, and angiogenesis. We discovered thatBacopa monnieriextract can increase migration of fibroblasts via modulating the size and number of focal adhesions.Bacopa monnieri-mediated changes in focal adhesions are dependent on α5β1 integrin activation and subsequent phosphorylation of Focal Adhesion Kinase (FAK). Altogether our results suggest thatBacopa monnieriextract could enhance the wound healing rate via modulating fibroblast migration in the wound bed.
Geographic migration and vaccine-induced fitness changes ofStreptococcus pneumoniae
Authors: Belman Sophie , Lefrancq Noémie , Nzenze Susan , Downs Sarah , du Plessis Mignon , Lo Stephanie ,   The Global Pneumococcal Sequencing Consortium, McGee Lesley , Madhi Shabir A. , von Gottberg Anne , Bentley Stephen D. , Salje Henrik 
Published: January 2023

AbstractStreptococcus pneumoniaeis a leading cause of pneumonia and meningitis worldwide. Many different serotypes co-circulate endemically in any one location. The extent and mechanisms of spread, and vaccine-driven changes in fitness and antimicrobial resistance (AMR), remain largely unquantified. Using geolocated genome sequences from South Africa (N=6910, 2000-2014) we developed models to reconstruct spread, pairing detailed human mobility data and genomic data. Separately we estimated the population level changes in fitness of strains that are (vaccine type, VT) and are not (non-vaccine type, NVT) included in the vaccine, first implemented in 2009, as well as differences in strain fitness between those that are and are not resistant to penicillin. We estimated that pneumococci only become homogenously mixed across South Africa after about 50 years of transmission, with the slow spread driven by the focal nature of human mobility. Further, in the years following vaccine implementation the relative fitness of NVT compared to VT strains increased (RR: 1.29 [95% CI 1.20-1.37]) – with an increasing proportion of these NVT strains becoming penicillin resistant. Our findings point to highly entrenched, slow transmission and indicate that initial vaccine-linked decreases in AMR may be transient.One-Sentence SummaryWe describe geographic migration, and fitness dynamics conferred by NVT strains and AMR, for the globally endemic pathogenStreptococcus pneumoniae.
SARS-CoV-2 Omicron XBB.1.5 May Be a Variant That Spreads More Widely and Faster Than Other Variants
Authors: Sugano Aki , Kataguchi Haruyuki , Ohta Mika , Someya Yoshiaki , Kimura Shigemi , Maniwa Yoshimasa , Tabata Toshihide , Takaoka Yutaka 
Published: January 2023

ABSTRACTIn this research, we aimed to predict the relative risk of the recent new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the basis of our previous research. We first performed molecular docking simulation analyses of the spike proteins with human angiotensin-converting enzyme 2 (ACE2) to determine the binding affinities to human cells of three new variants of SARS-CoV-2: Omicron BQ.1, XBB, and XBB.1.5 We then investigated the three variants to discover the evolutionary distance of the spike protein gene (S gene) from the Wuhan, Omicron BA.1, and Omicron BA.4/5 variants, to understand the changes in the S gene.The results indicated that the XBB.1.5 variant had the highest binding affinity of the spike protein with ACE2 and the longest evolutionary distance of the S gene. Thisin silicoevidence suggested that the XBB.1.5 variant may produce infections that spread more widely and faster than can infections of preexisting variants.
Application of gamification for sustainable construction: an evaluation of the challenges
Authors: Oke Ayodeji Emmanuel , Aliu John , Tunji-Olayeni Patience , Abayomi Timilehin 
Published: January 2023

Purpose
This paper aims to identify and evaluate the challenges affecting the adoption of gamification practices in developing countries through the lens of the Nigerian construction industry.

Design/methodology/approach
A scoping literature review was conducted through which challenges to the adoption of gamification practices were identified, which helped in the formulation of a questionnaire survey. Data was obtained from construction professionals including architects, builders, engineers and quantity surveyors. Retrieved data were analyzed using several statistical tools such as percentages, frequencies, mean item scores (MIS) and exploratory factor analyses.

Findings
Based on the MIS ranking results, the top five significant challenges to the adoption of gamification were lack of capacity and expertise, lack of budgeting for innovation, lack of technical infrastructure, hesitation to adopt and limited internet connectivity. Through factor analysis, the challenges identified were categorized into five principal clusters, namely, organizational challenges, technical-related challenges, human-related challenges, data security challenges and economic challenges.

Practical implications
The identification and evaluation of the key challenges hindering the adoption of gamification practices would help construction organizations and stakeholders to understand the need to embrace and implement the concept into their activities, operations and processes to improve the engagement and motivation levels of employees.

Originality/value
To the best of the authors’ knowledge, this study is the first of its kind in the study area to identify and evaluate the challenges affecting the adoption of gamification practices using a structured quantitative approach.
Project cost control system and enabling-factors model: PLS-SEM approach and importance-performance map analysis
Authors: Le An Thi Hoai , Sutrisna Monty 
Published: January 2023

PurposeThis paper reports the developments of a project cost control system (PCCS) for construction projects to (1) measure its current level of cost control maturity, (2) examine the relationships between elements within PCCS processes and (3) identify improvement areas.Design/methodology/approachThis study adopts a mixed approach of descriptive analysis and partial least squares structural equation modelling (PLS-SEM) to measure the current maturity level of PCCS and evaluate the relationships between elements within PCSS to identify improvement areas. Further importance-performance matrix analysis (IPMA) of priority constructs was conducted to improve a target construct and identify the most important areas of specific actions at indicator levels. The results of IPMA revealed the contrast that has the greatest importance on the performance of others so that the recommendations can be made accordingly. Data collected in New Zealand were used to develop the research model.FindingsThis study develops structural and measurement models with the constructs including pre-control, in-control and post-control processes, enablers and their proposed interrelationships. Then, data from survey of 184 experienced project cost control team members reveal that post-control has the lowest maturity or weakest areas in the PCCS. Data analysis facilitated by PLS-SEM confirmed that all the constructs in the structural model have positive and significant relationships with each other and suggested that systematic cost analysis reports, communication, skills and experience, defining roles and responsibilities, and top management's support should be the highest priority for improving the PCCS in a more effective manner.Originality/valueThis study presents one of the earliest attempts to develop and test an integrated model that links sub-processes in PCCS and their enablers. Secondly, this research adds to the construction project management literature by empirically verifying the roles of enablers in enhancing maturity level of PCCS.
An optimization model for collaborative logistics among carriers in vehicle routing problems with cross-docking
Authors: Ghomi Vahid , Gligor David , Shokoohyar Sina , Alikhani Reza , Ghazi Nezami Farnaz 
Published: January 2023

PurposeCollaborative Logistics (CL) and merging operations are crucial strategies for reducing costs and improving service in transportation companies. This study proposes a model for optimizing efficiency in supply chain networks through inbound and outbound Collaborative Logistics implementation among the carriers in centralized, coordinated networks with cross-docking.Design/methodology/approachA mixed-integer non-linear programming model is developed to determine the optimal truck-goods assignment while gaining economies of scale through mixing multiple less-than-truckload (LTL) products with different weight-to-volume ratios. Unlike the previous studies that have considered Collaborative Logistics from the cost and profit-sharing perspective, the proposed model seeks to determine an appropriate form of Collaborative Logistics in the VRP.FindingsThis article shows that in a three-echelon supply chain consisting of a set of suppliers, a set of customers and a cross-docking terminal, partial collaboration among the inbound carriers and outbound carriers outperforms no/complete collaboration. This approach enhances the supply chain efficiency by minimizing the total transportation costs, the total transportation miles and the total number of trucks and maximizing fleet utilization. While addressing the four points, the role of collaborative logistics among the carriers was discussed. In a three-echelon SC consisting of a set of suppliers, a set of customers and a cross-docking terminal, partial collaboration among the inbound carriers and outbound carriers outperforms no/complete collaboration. Using a combination of experimental analysis and optimization process, it was recommended that managers be cautious that too much (full or complete) or no collaboration can result in SC performance deterioration.Originality/valueThe suggested approach enhances the supply chain efficiency by minimizing the total transportation costs, the total transportation miles and the total number of trucks and maximizing fleet utilization. While addressing the four points, the role of Collaborative Logistics among the carriers was discussed.
Bridging sustainability knowledge management and supply chain learning: evidence through buyer selection
Authors: Silva Minelle E. , Pereira Michele M.O. , Boffelli Albachiara 
Published: January 2023

PurposeTo better understand the supplier's role in promoting supply chain sustainability (SCS), the authors investigated the learning process as it relates to sustainability knowledge. Through the lens of the knowledge-based view, they understand the shift of sustainability knowledge from rhetoric to common knowledge existent between suppliers and buyers.Design/methodology/approachA case study method was employed to study sustainability knowledge learning between a key global coffee supplier and its geographically dispersed buyers. The research was developed with data collected from 2019 through 2021. Interviews and secondary data were analyzed using both deductive and inductive approaches.FindingsResults were organized to demonstrate how the supplier developed and transferred its own sustainability knowledge within supplier–buyer dyads. The authors uncovered that buyer selection was a vital strategy used to appropriate the value created to ensure SCS learning. Four learning stages were analyzed, and while the results indicated that all buyers acquired knowledge, they also showed that only four distributed it. Moreover, different levels of interpretation were identified, two of which were associated with a low level of understanding of the meaning of sustainability knowledge. In addition, the data provided little evidence of organizational memory. All links were guided by common sustainability knowledge learned through multiple learning loops between the supplier's knowledge management and buyers' SCS learning, thus boosting sustainability in the coffee supply chain.Practical implicationsA greater understanding of how sustainability knowledge is learned in supply chains helps managers develop better SCS strategies.Originality/valueUnlike previous research, this paper illustrates that common sustainability knowledge is key to SCS implementation, which is made possible by carefully selecting buyers and by facilitating sustainability knowledge learning through two-way interactions.
Culture and innovation: a human emancipation perspective
Authors: Yeganeh Hamid 
Published: January 2023

PurposeThis study aims at offering a comprehensive thesis about the relationship between different cultural values and innovativeness.Design/methodology/approachBuilding on the human emancipation perspective and using data from Hofstede’s, Schwartz’s, and Inglehart’s cultural frameworks, the authors conduct a cross-national investigation into the effects of cultural values on national innovativeness.FindingsThe analyses show that emancipatory cultural dimensions such as rationality, secularity, self-expression, individualism, low uncertainty avoidance, long-term orientation, mastery and autonomy have significantly positive associations with national innovativeness. The opposing cultural values, such as traditionalism, religiosity, survival, collectivism, high uncertainty avoidance, short-term orientation, harmony and conservatism, have negative associations with national innovativeness.Originality/valueThis study contributes to the literature by putting forward a comprehensive and theory-driven explanation of the relationship between cultural values and innovativeness, by using all of Hofstede’s, Schwartz’s and Inglehart’s dimensions, by incorporating ethnic, linguistic and religious diversities and by applying alternative measures of the national innovativeness.
Outcomes of psychological contract breach for banking employees during COVID-19 pandemic: moderating role of learned helplessness
Authors: Karani Mehta Anushree , Purohit Divyang , Trivedi Payal , Panda Rasananda 
Published: January 2023

PurposeThe present study aims to understand the relationship between psychological contract breach (PCB) and outcome variables with mediation role of job stress, psychological empowerment and moderating role of learned helplessness.Design/methodology/approachDescriptive cross-sectional research design was deployed. Data were collected from the Indian public sector bank employees, especially from those who are regularly going to the banks even during the lockdown situation. A total of 239 respondents were contacted via online and offline method.FindingsThe authors reported that bank employees feel the breach of psychological contract which induced job stress. Further, job stress negatively impacts their psychological empowerment and psychological empowered employees exhibit increased innovative behavior and well-being. The authors also found that job stress and psychological empowerment mediated the relationship between PCB and outcome variables, and learned helplessness moderates this relationship. The current study captures the psychological response of employees during the pandemic era.Originality/valueThe study also highlights that during the pandemic, when majority of the employers have given work from home, the public sector employees were regularly going to the banks with fragile mindset. The banks' managers and HR managers can also understand that how the fulfillment of expectations is important not only for employee well-being but also for the health of the organization.