bluuebunny/crossref_metadata_2025_split huggingface dataset:
HISTOPATHOLOGICAL CHANGES OF THE UMBILICAL CORD IN COMPLİCATED PREGNANCIES
Authors: KAYA Seval , NERGİZ Yusuf , TURGUT A. Kadir 
Published: May 2023

Preeclampsia, hellp
syndrome and gestational diabetes are quite common complication in pregnancy. In this study,ıt is aimed to compare the thickness of
basal lamina
and vascular structure of umbilical cord. by
comparing preeclampsia,
hellp syndrome, gestational diabetes and normal pregnant women.
In
the study, sanples were taken from postpartium
umbilical cords of
normal pregnant
women, preeclampsia, gestational diabetes and pregnant women diagnosed
with
hellp syndrome. Umblical cord samples taken during maternity, followed by routine paraffin through fixing 10% neutral formalin. In this performed as totally sixty
facts through
taking fifteen
umbilical cords from each of four groups, Hematoxyline-eosin, Masson trichrom, periodic
acid Schiff,
and toluidine
blue stain were applied
on to the serial histological sections obtained from paraffin blocks. The obtained slides
were evaluated
with
Zeiss Imager 2 Microscope by taking micrographs. Morphometric measurements were statistically analyzed with SPSS software. In cases of gestational diabetes due to increased collagen production, thickening and excessive narrowing of the lumen and differentiation of Wharton jelly were observed in the umbilical artery. Differentiations are observed in the vacuolization of smooth muscle cells and muscle fibers that make up the media while camber and damage are observed in
endothelial. The most significant structural changes of preeclamptic placentas are obtained
as
the thickening
and
the endothelial injury. In the umbilical cord artery medial layer, due to
the
growth of collagen fiber. In HELLP
cases due to the increase in media diffuse edema
between muscle fibers and in collagen,
the
thickening of umbilical
artery wall and the
narrowing of luminal are seen
as extremely significant. Another striking symptom
with HELLP cases is the observing of
intramuscular hemorrhages in the umbilical artery wall as an
unattained case in preeclampsia and gestational diabetes. In most of the cases, differentiation in Wharton jelly, metaplastic changes in the amniotic epithelium and thickening
of the basal
lamina are observed.
PENGEMBANGAN APLIKASI PEMBELAJARAN BAHASA ARAB UNTUK PEMULA BERBASIS WEB
Authors: Febriana Dina , Iswari Lizda 
Published: May 2023

Pembelajaran bahasa arab di Indonesia masih memiliki tantangan dalam metode penyampaiannya. Hal ini dapat dilihat pada media pembelajaran Bahasa Arab sebagian besar masih terpaku pada buku dan metode penyampaian tradisional berupa ceramah satu arah. Oleh karena itu diperlukan media pembelajaran yang dapat meningkatkan motivasi pelajar untuk mempelajari bahasa arab. Dalam penelitian ini dikembangkan sebuah aplikasi pembelajaran bahasa arab untuk pemula berbasis web dengan metode penelitian research and development. Metode penelitian tersebut dilaksanakan melalui studi literatur dan studi lapangan untuk dapat menemukan inti permasalahan sehingga dapat merancang kebutuhan aplikasi sebagai solusi efektif. Tujuan dari penelitian ini adalah dibangunnya sebuah aplikasi pembelajaran bahasa arab untuk pemula berbasis web dengan materi berupa pengenalan huruf, pengenalan angka, kosakata, dan penyusunan kalimat. Materi akan disajikan dalam bentuk teks, audio, dan gambar sehingga dapat meningkatkan ketertarikan pelajar dalam mempelajari dan memahami dasar bahasa arab. Selain itu, aplikasi dikembangkan berbasis web sehingga pengguna dapat mengakses aplikasi menggunakan berbagai platform. Setelah aplikasi berhasil dikembangkan, kemudian diberikan kepada sejumlah responden untuk mengetahui tingkat kelayakan sistem. Hasil pengujian menggunakan metode pengujian system usability scale dan menghasilkan predikat “Good” sehingga masih layak untuk digunakan para pelajar.
Kata kunci-aplikasi berbasis web, bahasa arab, media pembelajaran
Selenyum Dioksit ve Paklitaksel Kombinasyonunun MDA-MB-231 Meme Kanseri Hücre Hattı Üzerine Etkisinin Değerlendirilmesi
Authors: BULUT Seda , ÖNDER Gözde Özge , YAY Arzu 
Published: May 2023

Amaç: Bu çalışmanın amacı, bitkisel kökenli bileşik olan selenyum dioksit (SEL) ile kemoterapötik ilaç olan paklitaksel’in (PTX) kombine uygulamasının MDA-MB-231 meme kanseri hücre hattı üzerine etkisinin belirlenmesidir.
Gereç ve Yöntemler: Çalışmamızda öncelikle SEL’in yarı maksimal inhibitör konsantrasyonunun belirlenmesi amacıyla MTT testi yapıldı. Deney grupları SEL1, SEL2, SEL3, PTX, SEL1+PTX, SEL2+PTX ve SEL3+PTX şeklinde oluşturulduktan sonra apoptoz TUNEL metodu, NF-kB ekspresyonu immunofloresan boyama metodu ile hücre canlılığı ise MTT analizi ile gösterildi.
Bulgular: SEL’in meme kanseri hücrelerinde hücre canlılığını azalttığı gösterildi. SEL1, SEL2, SEL3, PTX gruplarında kontrole göre apoptozun arttığı görüldü (p0,999, p>0,999, p=0,725).
Sonuç: Mevcut çalışmada MDA-MB-231 meme kanseri hücre dizisinde SEL ve PTX’in antagonistik etkiye sahip oldukları belirlenmiştir.
Sociodemographic and Clinical Characteristics of Children and Adolescents Applying to the Child Psychiatry Outpatient Clinic in Düzce
Authors: ÖZ Büşra , KIVRAK Asil Can 
Published: May 2023

Aim: In our study, it was aimed to examine the sociodemographic characteristics, symptoms, psychiatric disorders of children and adolescents who applied for the first time to a university hospital child and adolescent psychiatry outpatient clinic.
Material and Methods: The files of 724 children and adolescents who applied to our outpatient clinic were reviewed retrospectively. 211 patients evaluated for the first time in our outpatient clinic were included in the study. Sociodemographic characteristics, first admission complaints, psychiatric diagnoses and treatments of the patients were evaluated.
Results: The mean age of the patients included in the study were 9.90±4.07 years. It was determined that 117 (55.5%) of the patients had a previous psychiatric diagnosis. The most common complaints at presentation were attention deficit/hyperactivity (45%), irritability (20.4%), and fear-anxiety (15.2%). It was determined that 191 (90.5%) of the patients had at least one psychiatric disorders and 49 (23.2%) had at least two psychiatric disorders. The most common psychiatric disorders were ADHD (54.9%), AD (20.4%), BD (10.4%) and MDD (8.3%). It was determined that 46% of the patients received psychiatric treatment, and methylphenidate (62.9%) was the most common use.
Conclusion: In our study, the most common complaint of the patients was attention deficit/hyperactivity, and the most common diagnosis was ADHD. Our results are similar to other studies. The presence of comorbidity increases the severity of the disorders and causes serious deterioration in psychosocial functionality. Diagnoses between genders and comorbidities should be carefully evaluated in patients applying to child and adolescent psychiatry.
The Frequency of Pancreatic Enzyme Elevations and Effect on Disease Severity in COVID-19
Authors: ALKAN Sevil , GÖKÇE Oruç Numan , ÇİNPOLAT Havva Yasemin 
Published: May 2023

Aim: In this study, we aimed to evaluate the frequency of pancreatic enzyme elevations in COVID-19-infected patients and to examine the effect on disease severity.
Material and Methods: A total of 1249 patients who hospitalized with COVID-19 infection were included. The frequency of pancreatic enzyme elevations and the effect on disease severity in patients infected with COVID-19 were investigated.
Results: The pancreatic enzyme elevations (amylase/lipase or both) were detected in 32 of 1249 patients (incidence 2.96%). 32 cases with a mean age of 64.97±5.63 years were included in this study. 30 (93.75%) of the cases were men’s gender. 31 (96.87%) of them had elevated amylase levels, 26 (81.25%) had elevated lipase levels and 25 (78.12%) of them had elevated both amylase and lipase levels. Only 10 (31.25%) of them tested radiological for acute pancreatitis (AP) and there was no radiological finding compatible with AP in any of the limited numbers of abdominal computerized tomography scans performed. 18 (56.25%) of the patients were transferred to the intensive care unit due to clinical worsening and mortality developed in 13 (40.62%) patients. The mean age of the deceased cases was 66.4±6.6 years and there was no statistically significant difference between deceased and survived COVID-19 patients (>0.05).
Conclusion: The median lymphocyte count was lower, and the median AST, ALT, and lipase levels were higher in the deceased group. Perhaps close clinical follow-up of patients with pathological findings in these values and radiological imaging, if necessary, may be beneficial in the method of the disease.
Keywords: COVID-19; disease severity; pancreatic enzyme elevations.
Anticancer Activity of Endemic Phlomis Extracts in HCT116 Human Colon Cancer Cells
Authors: KURMAN Yener , PASİN Ozge 
Published: May 2023

Aim: Previous studies have reported that Phlomis russeliana shows cytotoxic effects against several cancer cell lines; however, its anti cancer activity on HCT-116 cancer cells has not yet been investigated. Therefore, the present study is designed to explore anti-cancer properties of Phlomis russeliana against HCT-116 human colon cancer cell line and HUVEC normal cell line.
Material and Methods: HCT-116 cells and HUVECs treated with different concentrations of Phlomis russeliana (2, 4, 6, 8 and 10 mg/ml) and cell viability evaluated by the MTT assay. Anti-migratory and anti-colonigenic effects of Phlomis russeliana were assessed with wound healing and colony formation assays respectively. Quantitative determination of total antioxidant status (TAS), total oxidant status (TOS) and caspase-3 activation were performed with colorimetric Elisa kits.
Results: Phlomis russeliana significantly decreased cell viability of HCT-116 cells in a concentration dependent and showed weaker toxicity against normal HUVECs. Pholomis russeliana significantly inhibited migration and colony formation potential of HCT-116 cells. A significant increase in caspase-3 activation was observed after treatment with Phlomis russeliana. Phlomis russeliana did not significantly affect the TAS and TOS level in HCT-116 cells.
Conclusion: These results revealed that Phlomis russeliana showed anti-cancer activity in human colon cancer cells, through the suppression of colony formation, inhibition of migration and induction of caspase-3 activation. Phlomis russeliana, could be a promising source for the development of new anti-cancer agents against cancer.
Batı Ege Bölgesinde α-Talasemi Genotipleri ve α-Talasemi Genotip Frekansı
Authors: BARIŞ Savaş , YAVAŞ Cüneyd , BALASAR Özgür , GÖRDÜ Zülfükar , DOĞAN Mustafa , ERÖZ Recep 
Published: May 2023

Amaç: Yaygın görülen bir tek gen hastalığı olan Alfa talasemi, α-globin zincirinin kusurlu sentezi ile ortaya çıkar. Globin genlerindeki bozukluklara bağlı olarak çok geniş bir klinik spektruma yayılan bu hastalıkta çok sayıda belirlenmemiş taşıyıcı olduğu düşünülmektedir. Klinik semptomu olmayan sessiz taşıyıcılardan, rahim içinde ölüme yol açan şiddetli anemi ile kendini gösteren, çok değişken bulgulara sahip genetik bir hastalıktır. Bu çalışmada bu amaçla alfa globin gen mutasyonu sıklığının ve tiplerinin bulunması ve varyasyon saptanan bireylerdeki fenotipik etkiyi görmek amaçlandı.
Gereç ve Yöntemler: HBA1 ve HBA2 genlerindeki intron bölgelerini çevreleyen tüm kodlama bölgesi sanger dizileme ile tespit edildi. Delesyonlar ve duplikasyonlar multipleks ligasyona bağımlı prob amplifikasyonu (MLPA) ile mutasyonlar tespit edildi.
Bulgular: Bölgemizde en sık rastlanan mutasyon tipi olan -3,7 / (%23,18), 3.7 kb’lık delesyon çalışmamızda da en sık olarak görülürken, diğer mutasyonların dağılımı ise --3,7 (%6,82), -3,7/-- MED (%0,91), --MED (%6,82), --20,5 (3,15), --SEA (%1,36), -4,2 (%0,95), triplikasyon (%0,45) ve nükleotid değişimleri (%4,55) olarak tespit edilmiştir.
Sonuç: Mevcut bilgiler ışığında genotipin fenotipe yansımasının da farklılıklar olması nedeniyle taşıyıcı bireylerin tesbit edilmesi ve genotip fenotip ilişkisinin netleştirilmesi açısından daha geniş popülasyon taramasına ihtiyaç duyulmaktadır. Toplumu alfa talasemi ve ağır klinik seyreden genetik hastalıklar hakkında bilinçlendirmek için taşıyıcı bireylere genetik danışmanlık verilmesi ve genetik çalışmalara ağırlık verilmesi bir gerekliliktir.
Proteomic analysis of urinary extracellular vesicles highlights specific signatures for patients with primary aldosteronism
Authors: Bertolone Lorenzo , Castagna Annalisa , Manfredi Marcello , De Santis Domenica , Ambrosani Francesca , Antinori Elisa , Mulatero Paolo , Danese Elisa , Marengo Emilio , Barberis Elettra , Veneri Mariangela , Martinelli Nicola , Friso Simonetta , Pizzolo Francesca , Olivieri Oliviero 
Published: May 2023

BackgroundUrinary extracellular vesicles (uEVs) can be released by different cell types facing the urogenital tract and are involved in cellular trafficking, differentiation and survival. UEVs can be easily detected in urine and provide pathophysiological information “in vivo” without the need of a biopsy. Based on these premises, we hypothesized that uEVs proteomic profile may serve as a valuable tool in the differential characterization between Essential Hypertension (EH) and primary aldosteronism (PA).MethodsPatients with essential hypertension (EH) and PA were enrolled in the study (EH= 12, PA=24: 11 Bilateral Primary Aldosteronism subtype (BPA) and 13 Aldosterone Producing Adenoma (APA)). Clinical and biochemical parameters were available for all the subjects. UEVs were isolated from urine by ultracentrifugation and analysed by Transmission Electron Microscopy (TEM) and nanotrack particle analysis (NTA). UEVs protein content was investigated through an untargeted MS-based approach. Statistical and network analysis was performed to identify potential candidates for the identification and classification of PA.ResultsMS analysis provided more than 300 protein identifications. Exosomal markers CD9 and CD63 were detected in all samples. Several molecules characterizing EH vs PA patients as well as BPA and APA subtypes were identified after statistical elaboration and filtering of the results. In particular, some key proteins involved in water reabsorption mechanisms, such as AQP1 and AQP2, were among the best candidates for discriminating EH vs PA, as well as A1AG1 (AGP1).ConclusionThrough this proteomic approach, we identified uEVs molecular indicators that can improve PA characterization and help in the gain of insights of the pathophysiological features of this disease. In particular, PA was characterized by a reduction of AQP1 and AQP2 expression as compared with EH.
Transcriptomic changes in liver transplant recipients with non-alcoholic steatohepatitis indicate dysregulation of wound healing
Authors: Pellegrina Diogo , Prayitno Khairunnadiya , Azhie Amirhossein , Pasini Elisa , Baciu Cristina , Fischer Sandra , Reimand Jüri , Bhat Mamatha 
Published: May 2023

BackgroundNon-alcoholic steatohepatitis (NASH) has become a leading indication for liver transplantation. However, it often recurs in the graft and can also arise de novo in individuals transplanted for other indications. Post-transplant NASH (PT-NASH) is more aggressive and leads to accelerated fibrosis. The mechanistic basis of PT-NASH has not yet been defined and no specific therapeutic strategies are currently available.MethodsHere, we profiled the transcriptomes of livers with PT-NASH from liver transplant recipients to identify dysregulated genes, pathways, and molecular interaction networks.ResultsTranscriptomic changes in the PI3K-Akt pathway were observed in association with metabolic alterations in PT-NASH. Other significant changes in gene expression were associated with DNA replication, cell cycle, extracellular matrix organization, and wound healing. A systematic comparison with non-transplant NASH (NT-NASH) liver transcriptomes indicated an increased activation of wound healing and angiogenesis pathways in the post-transplant condition.ConclusionBeyond altered lipid metabolism, dysregulation of wound healing and tissue repair mechanisms may contribute to the accelerated development of fibrosis associated with PT-NASH. This presents an attractive therapeutic avenue to explore for PT-NASH to optimize the benefit and survival of the graft.
Analysis of mRNA-miRNA-lncRNA differential expression in prediabetes/type 2 diabetes mellitus patients as potential players in insulin resistance
Authors: Ali Hebatalla Said , Kamel Marwa Mostafa , Agwa Sara H. A. , Hakeem Mohamed S. Abdel , Meteini Mahmoud Shawky El , Matboli Marwa 
Published: May 2023

IntroductionType 2 diabetes mellitus (T2DM) is a major global health concern. It usually develops gradually and is frequently preceded by undetectable pre-diabetes mellitus (pre-DM) stage. The purpose of this study was to identify a novel set of seven candidate genes associated with the pathogenesis of insulin resistance (IR) and pre-DM, followed by their experimental validation in patients’ serum samples.MethodsWe used the bioinformatics tools and through a two-step process, we first identified and verified two mRNA candidate genes linked to insulin resistance molecular pathogenesis. Second, we identified a non-coding RNAs related to the selected mRNAs and implicated in the insulin resistance molecular pathways followed by pilot study for the RNA panel differential expression in 66 patients with T2DM, 49 individuals with prediabetes and 45 matched controls using real time PCR.ResultsThe levels of expression of TMEM173 and CHUK mRNAs, hsa-miR (-611, -5192, and -1976) miRNAs gradually increased from the healthy control group to the prediabetic group, reaching their maximum levels in the T2DM group (p &lt;10-3), whereas the levels of expression of RP4-605O3.4 and AC074117.2 lncRNAs declined gradually from the healthy control group to the prediabetic group, reaching their lowest levels in the T2DM group (p &lt;10-3). TMEM173, CHUK mRNAs, hsa_miR (-611 &amp; -1976) and RP4-605O3.4 lncRNA were useful in distinguishing insulin resistant from insulin sensitive groups. miR_611 together with RP4-605O3.4 exhibited significant difference in good versus poor glycemic control groups.DiscussionThe presented study provides an insight about this RNA based STING/NOD/IR associated panel that could be used for PreDM-T2DM diagnosis and also as a therapeutic target based on the differences of its expression level in the pre-DM and T2DM stages.
Mendelian randomization indicates a causal contribution of type 2 diabetes to retinal vein occlusion
Authors: Huang Jian 
Published: May 2023

BackgroundRetinal vein occlusion (RVO) is a common retinal vascular disease that can cause severe visual impairment. Many observational studies have shown that type 2 diabetes (T2DM) is associated with RVO, but it remains unknown if the association is causal. The present study aimed to perform Mendelian randomization (MR) analyses to evaluate the causal contribution of genetically predicted T2DM to RVO.MethodsWe obtained summary-level data from a genome-wide association study meta-analysis including 48,286 cases and 250,671 controls for T2DM and from a genome wide association study of 372 cases and 182,573 controls in the FinnGen project for RVO. To verify the robustness of the results, an independent validation dataset for T2DM (12,931 cases and 57,196 controls) was used. In addition to the main MR analysis using the inverse variance weighted (fixed effect) approach, sensitivity analyses and multivariable MR adjusting for common risk factors of RVO were conducted.ResultsGenetically predicted T2DM was found to be causally associated with RVO risk (odds ratio (OR)=2.823, 95% confidence interval (CI): 2.072-3.847, P=4.868×10-11). This association was supported by sensitivity analyses using the weighted median (OR=2.415, 95% CI: 1.411-4.132, P=1.294×10-3), weighted mode (OR=2.370, 95% CI: 1.321-4.252, P=5.159×10-3), maximum likelihood (OR=2.871, 95% CI: 2.100-3.924, P=3.719×10-11), MR-PRESSO (OR=2.823, 95% CI: 2.135-3.733, P=5.150×10-10), and MR-Egger (OR=2.441, 95% CI: 1.149-5.184, P=2.335×10-2) methods. In addition, this association persisted in multivariable MR after accounting for common RVO risk factors (OR=1.748, 95% CI: 1.238-2.467, P=1.490×10-3). The MR analyses using the validation dataset obtained consistent results.ConclusionThis study indicates that genetically predicted T2DM may have a causal contribution to RVO. Future studies are required to elucidate the underlying mechanisms.
Nonalcoholic fatty liver disease and type 2 diabetes: an observational and Mendelian randomization study
Authors: Yu Yuetian , Yu Yuefeng , Wang Yuying , Chen Yi , Wang Ningjian , Wang Bin , Lu Yingli 
Published: May 2023

IntroductionNonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM) are both chronic multisystem diseases that cause tremendous health burdens worldwide. Previous epidemiological studies have found a bidirectional relationship between these two diseases; however, their causality remains largely unknown. We aim to examine the causal relationship between NAFLD and T2DM.MethodsThe observational analysis included 2,099 participants from the SPECT-China study and 502,414 participants from the UK Biobank. Logistic regression and Cox regression models were used to examine the bidirectional association between NAFLD and T2DM. Two-sample Mendelian randomization (MR) analyses were conducted to investigate the causal effects of the two diseases using summary statistics of genome-wide association studies from the UK Biobank for T2DM and the FinnGen study for NAFLD.ResultsDuring the follow-up, 129 T2DM cases and 263 NAFLD cases were observed in the SPECT-China study, and 30,274 T2DM cases and 4,896 NAFLD cases occurred in the UK Biobank cohort. Baseline NAFLD was associated with an increased risk of incident T2DM in both studies (SPECT-China: OR: 1.74 (95% confidence interval (CI): 1.12–2.70); UK Biobank: HR: 2.16 (95% CI: 1.82–2.56)), while baseline T2DM was associated with incident NAFLD in the UK Biobank study only (HR: 1.58). Bidirectional MR analysis showed that genetically determined NAFLD was significantly associated with an increased risk of T2DM (OR: 1.003 (95% CI: 1.002–1.004, p&lt; 0.001)); however, there was no evidence of an association between genetically determined T2DM and NAFLD (OR: 28.1 (95% CI: 0.7–1,143.0)).ConclusionsOur study suggested the causal effect of NAFLD on T2DM development. The lack of a causal association between T2DM and NAFLD warrants further verification.