bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety and microbiological activity of phage therapy in persons with cystic fibrosis colonized with Pseudomonas aeruginosa: study protocol for a phase 1b/2, multicenter, randomized, double-blind, placebo-controlled trial
Authors: Tamma Pranita D. , Souli Maria , Billard Michael , Campbell Joseph , Conrad Douglas , Ellison Damon W. , Evans Beth , Evans Scott R. , Greenwood-Quaintance Kerryl E. , Filippov Andrey A. , Geres Holly S. , Hamasaki Toshimitsu , Komarow Lauren , Nikolich Mikeljon P. , Lodise Thomas P. , Nayak Seema U. , Norice-Tra Carmelle , Patel Robin , Pride David , Russell Janie , Van Tyne Daria , Chambers Henry F. , FowlerJr Vance G. , Schooley Robert T. ,   for the Antibacterial Resistance Leadership Group
Published: December 2022

Abstract
Background
Bacteriophages (phages) are a promising anti-infective option for human disease. Major gaps remain in understanding their potential utility.

Methods
This is a randomized, placebo-controlled, double-blind study of a single dose of intravenous phage in approximately 72 clinically stable adult cystic fibrosis volunteers recruited from up to 20 US sites with Pseudomonas aeruginosa airway colonization. The single dose of phage consists of a mixture of four anti-pseudomonal phages. Six sentinel participants will be sequentially enrolled with dose escalation of the phage mixture by one log10 beginning with 4 × 107 plaque-forming units in an unblinded stage 1. If no serious adverse events related to the study product are identified, the trial will proceed to a double-blinded stage 2. In stage 2a, 32 participants will be randomly assigned to one of three phage dosages or placebo in a 1:1:1:1 allocation. An interim analysis will be performed to determine the phage dosage with the most favorable safety and microbiological activity profile to inform phage dosing in stage 2b. During stage 2b, up to 32 additional volunteers will be randomized 1:1 to the phage or placebo arm. Primary outcomes include (1) the number of grade 2 or higher treatment-emergent adverse events, (2) change in log10P. aeruginosa total colony counts in sputum, and (3) the probability of a randomly selected subject having a more favorable outcome ranking if assigned to receive phage therapy versus placebo. Exploratory outcomes include (1) sputum and serum phage pharmacokinetics, (2) the impact of phage on lung function, (3) the proportion of P. aeruginosa isolates susceptible to the phage mixture before and after study product administration, and (4) changes in quality of life.

Discussion
This trial will investigate the activity of phages in reducing P. aeruginosa colony counts and provide insights into the safety profile of phage therapy.

Trial registration
ClinicalTrials.gov NCT05453578. Registered on 12 July 2022.
Response letter to “Latent class analysis of 216 patients with adult-onset Still’s disease” by Sugiyama et al.
Authors: Delplanque Marion , Mekinian Arsène , Georgin-Lavialle Sophie 
Published: December 2022

AbstractSugiyama et al. recently described in “Latent class analysis of 216 patients with adult-onset Still’s disease,” baseline characteristics, laboratory tests, treatment, relapse, and death of adult-onset Still’s disease (AOSD) patients from a Japanese hospital. They identified two subgroups: Class 1 (n=155) with a younger age and typical symptoms of AOSD and Class 2 (n=61) with older patients and fewer typical symptoms of AOSD. In 2022, VEXAS (vacuoles, E1 enzyme, X-linked, autoinflammatory, somatic) syndrome, an established X-linked disease associated with a somatic mutation in UBA1, is considered as a differential diagnosis for AOSD particularly in elderly. These patients from Class 2 could benefit from more explorations for mild myelodysplasia and VEXAS.
Maintenance of methylation profile in imprinting control regions in human induced pluripotent stem cells
Authors: Pham A. , Selenou C. , Giabicani E. , Fontaine V. , Marteau S. , Brioude F. , David L. , Mitanchez D. , Sobrier M. L. , Netchine I. 
Published: December 2022

Abstract
Background
Parental imprinting is an epigenetic mechanism that leads to monoallelic expression of a subset of genes depending on their parental origin. Imprinting disorders (IDs), caused by disturbances of imprinted genes, are a set of rare congenital diseases that mainly affect growth, metabolism and development. To date, there is no accurate model to study the physiopathology of IDs or test therapeutic strategies. Human induced pluripotent stem cells (iPSCs) are a promising cellular approach to model human diseases and complex genetic disorders. However, aberrant hypermethylation of imprinting control regions (ICRs) may appear during the reprogramming process and subsequent culture of iPSCs. Therefore, we tested various conditions of reprogramming and culture of iPSCs and performed an extensive analysis of methylation marks at the ICRs to develop a cellular model that can be used to study IDs.

Results
We assessed the methylation levels at seven imprinted loci in iPSCs before differentiation, at various passages of cell culture, and during chondrogenic differentiation. Abnormal methylation levels were found, with hypermethylation at 11p15 H19/IGF2:IG-DMR and 14q32 MEG3/DLK1:IG-DMR, independently of the reprogramming method and cells of origin. Hypermethylation at these two loci led to the loss of parental imprinting (LOI), with biallelic expression of the imprinted genes IGF2 and DLK1, respectively. The epiPS™ culture medium combined with culturing of the cells under hypoxic conditions prevented hypermethylation at H19/IGF2:IG-DMR (ICR1) and MEG3/DLK1:IG-DMR, as well as at other imprinted loci, while preserving the proliferation and pluripotency qualities of these iPSCs.

Conclusions
An extensive and quantitative analysis of methylation levels of ICRs in iPSCs showed hypermethylation of certain ICRs in human iPSCs, especially paternally methylated ICRs, and subsequent LOI of certain imprinted genes. The epiPS™ culture medium and culturing of the cells under hypoxic conditions prevented hypermethylation of ICRs in iPSCs. We demonstrated that the reprogramming and culture in epiPS™ medium allow the generation of control iPSCs lines with a balanced methylation and ID patient iPSCs lines with unbalanced methylation. Human iPSCs are therefore a promising cellular model to study the physiopathology of IDs and test therapies in tissues of interest.
Prenatal diagnosis of fetal midgut volvulus: a case report
Authors: Jain Neelam , Awal Sandeep Singh , Biswas Som , Ghosh Tanushree 
Published: December 2022

Abstract
Background
Fetal midgut volvulus is an uncommon yet potentially life-threatening condition. Prenatal diagnosis may pose a challenge, due to the paucity of specific signs and symptoms. Timely prenatal diagnosis of this condition is imperative to prevent fetal mortality and morbidity.

Case presentation
We present a rare case report of fetal midgut volvulus, malrotation, and intestinal obstruction at 32 weeks of gestation in a 31-year-old multigravida Indian patient who presented with decreased fetal movements. Fetal ultrasound revealed midgut volvulus with proximal bowel obstruction and polyhydramnios. The patient underwent emergency surgery, which revealed intestinal malrotation and confirmed the diagnosis of midgut volvulus. Untwisting of the volvulus was done followed by Ladd’s procedure. Follow-up postoperative ultrasound was unremarkable.

Conclusions
Delay in the diagnosis of fetal midgut volvulus leads to poor fetal and maternal outcomes. Hence, it is vital for radiologists, sonologists, and obstetricians to be aware of this condition while performing fetal sonography. Prompt diagnosis and surgical intervention are vital to reduce the morbidity and mortality associated with this condition.
Effect of the initial pH on the anaerobic digestion process of dairy cattle manure
Authors: Castro-Ramos Job Jonathan , Solís-Oba Aida , Solís-Oba Myrna , Calderón-Vázquez Carlos Ligne , Higuera-Rubio Jesús Mireya , Castro-Rivera Rigoberto 
Published: December 2022

AbstractAnaerobic digestion (AD) has recently been studied to obtain products of greater interest than biogas, such as volatile fatty acids (VFAs) and phytoregulators. The effect of the initial pH of cow manure and the fermentation time of the AD on the microbial composition, VFAs, indole-3-acetic acid (IAA) and gibberellic acid (GA3) production was evaluated. The cow manure (7% solids) was adjusted to initial pH values of 5.5, 6.5, 7.5, and 8.5, and the AD products were analyzed every four days until day 20. The initial pH and the fermentation time had an important effect on the production of metabolites. During AD, only the hydrolytic and acidogenic stages were identified, and the bacteria found were from the phyla Firmicutes, Bacteroidetes, Actinobacteria, and Spirochaetes. The most abundant genera produced in the four AD were Caproiciproducens, Clostridium sensu stricto 1, Romboutsia, Paeniclostridium, Turicibacter, Peptostreptococcaceae, Ruminococcaceae and Fonticella. The highest amount of VFAs was obtained at pH 8.5, and the production of the acids was butyric > acetic > propionic. The maximum production of GA3 and IAA was at an initial pH of 6.5 on day 20 and a pH of 5.5 on day 4, respectively. There was a strong correlation (> 0.8) between the most abundant microorganisms and the production of VFAs and GA3. The anaerobic digestion of cow manure is a good alternative for the production of VFAs, GA3 and IAA.
Transcriptomic and enzymatic analysis reveals the roles of glutamate dehydrogenase in Corynebacterium glutamicum
Authors: Ge Fanglan , Sun Jingkun , Ren Yao , He Bing , Li Jiao , Yang Sen , Li Wei 
Published: December 2022

AbstractGlutamate dehydrogenase (Gdh), catalyzing the reversible conversion between 2-oxoglutarate and glutamate, plays an important role in the connection of nitrogen and carbon metabolism. Yet little is known about these enzymes in the amino acid-manufacturing Corynebacterium glutamicum. In the present study, we firstly identified the enzymatic characteristics of two Gdhs (GdhA and GdhB). The results showed that both GdhA and GdhB prefers NADPH as a coenzyme and have higher affinity for 2-OG than glutamate. The growth characteristics of gdhAΔ mutant and gdhBΔ mutant, gdhABΔ mutant showed GdhA serves as the main conduit for ammonium assimilation, and GdhB is the main glutamate- metabolizing enzyme in C. glutamicum. The full-genome transcriptomic analysis was used to investigate physiological response of C. glutamicum to the glutamate as nitrogen source, and gdh deletion. The results showed that the nitrogen starvation response was elicited when glutamine served as the sole nitrogen source. gdhAΔBΔ double deletion trigger a partially deregulated nitrogen starvation response, in which genes involved in nitrogen assimilation showed obviously upregulated in a certain extent. On the other hand, the genes of phosphotransferase system (PTS) and glycolysis pathway, most genes in pentose phosphate pathway were significantly upregulated, indicating that gdh deficiency initiated the enhancement of the absorption and metabolism of carbon sources. We believed that our results in this study will give new insights on the molecular mechanism of Gdh activity cross-talks with carbon and nitrogen metabolism, also setting a new background for further flux redistribution applied research of biotechnological interest.
Performance analysis on the speed spectrum estimation based on dual-HFM waveform
Authors: Wei Runyu , Li Xuan , Fu Yubin , Ma Xiaochuan 
Published: December 2022

AbstractReal-time Doppler estimation of sonar signals is of great significance to underwater communication and detection. The Hyperbolic-Frequency-Modulated (HFM) waveform-based speed spectrum estimation is one of the modern methods for underwater Doppler estimation, and this paper presents a detailed analyses of the derivation and the performance of this method. The analyzing results show the robustness of the method on multipath channels and multi-highlight targets and provide the main lobe width of the speed spectrum. Moreover, the paper examines the spectral properties of the HFM signal from the frequency-domain Doppler invariance property, which could provide insights for better understanding and utilization of this Doppler invariant waveform.
Hemodiafiltration combined with polymyxin B-immobilized fiber column direct hemoperfusion is effective for acute postoperative exacerbation of interstitial pneumonia: a case report
Authors: Yokoyama Tatsuro , Tamura Takahiro , Nakashima Harunori , Ando Morihide , Kikkawa Koshiro , Ito Ryohei 
Published: December 2022

Abstract
Background
Postoperative acute exacerbation of interstitial pneumonia has a high mortality rate; however, its treatment methods have not been standardized.

Case presentation
A 72-year-old man with rheumatoid arthritis developed acute respiratory failure about 3 weeks after lung cancer surgery. There were increased diffuse frosted shadows in both lung fields. His condition was diagnosed as an acute exacerbation of interstitial pneumonia associated with rheumatoid arthritis, and he was started on steroid pulse therapy; however, his respiratory condition deteriorated. He was urgently intubated and started on veno-venous extracorporeal membrane oxygenation. Further, intensive care, including blood purification therapy, was initiated. The blood purification therapy comprised a combination of hemodiafiltration and 6-h polymyxin B-immobilized fiber column direct hemoperfusion. The patient was weaned off veno-venous extracorporeal membrane oxygenation, extubated, and discharged from the intensive care unit on the ninth day.

Conclusions
Blood purification therapy was effective for acute exacerbation of interstitial pneumonia.
Gap selection and steering during obstacle avoidance in pigeons
Authors: Pérez-Campanero Antolín Natalia , Taylor Graham K. 
Published: December 2022

ABSTRACTThe ability of birds to fly through cluttered environments has inspired biologists interested in understanding its underlying mechanisms, and engineers interested in applying its underpinning principles. To analyse this problem empirically, we break it down into two distinct, but related, questions: How do birds select which gaps to aim for? And how do they steer through them? We answered these questions using a combined experimental and modelling approach, in which we released pigeons (Columbia livia domestica) inside a large hall with an open exit separated from the release point by a curtain creating two vertical gaps – one of which was obstructed by an obstacle. We tracked the birds using a high-speed motion capture system, and found that their gap choice seemed to be biased by their intrinsic handedness, rather than determined by extrinsic cues such as the size of the gap or its alignment with the destination. We modelled the pigeons' steering behaviour algorithmically by simulating their flight trajectories under a set of six candidate guidance laws, including those used previously to model target-oriented flight behaviours in birds. We found that their flights were best modelled by delayed proportional navigation commanding turning in proportion to the angular rate of the line-of-sight from the pigeon to the midpoint of the gap. Our results are consistent with this being a two-phase behaviour, in which the pigeon heads forward from the release point before steering towards the midpoint of whichever gap it chooses to aim for under closed-loop guidance. Our findings have implications for the sensorimotor mechanisms that underlie clutter negotiation in birds, uniting this with other kinds of target-oriented behaviours including aerial pursuit.
Χημεία στην Καθημερινή Ζωή
Authors: Kotsali Ilia , Kavouras Ermis 
Published: December 2022

Αντικείμενο της παρούσας έρευνας, την οποία πραγματοποιήσαμε στο πλαίσιο του μαθήματος της Χημείας στην Γ΄ τάξη Γυμνασίου, αποτέλεσε η ασφαλής και ποιοτική επιλογή της οδοντόκρεμας για τη διατήρηση και βελτίωση της στοματικής υγείας και αισθητικής, καθώς και η άποψη των φίλων και μαθητών του σχολείου μας για το ζήτημα αυτό. Η ομάδα μας επέλεξε να ασχοληθεί με το θέμα: «Πόσο καλά γνωρίζετε την οδοντόκρεμά σας;» γιατί είναι ένα ζήτημα το οποίο άπτεται της στοματικής υγιεινής και της ευημερίας των ατόμων, ενώ συγχρόνως έχει και περιβαλλοντικές συνέπειες. Το κεντρικό ερευνητικό μας ερώτημα ήταν: Πως μπορούμε να ξεχωρίσουμε ποια οδοντόκρεμα είναι κατάλληλη για εμάς; Αναζητήσαμε πληροφορίες, κυρίως μέσω του διαδικτύου, σχετικά με τη χημική σύσταση των σκευασμάτων οδοντόκρεμας και τις βασικές κατηγορίες στις οποίες διαχωρίζονται. Διερευνήσαμε κατά πόσο είναι ασφαλείς για τον ανθρώπινο οργανισμό και τον βαθμό στον οποίο το υλικό της συσκευασίας τους επιβαρύνει το περιβάλλον. Με βάση τη γνώση που αποκομίσαμε, διεξήγαμε μία ενδεικτική κοινωνική έρευνα, χρησιμοποιώντας τα μέσα κοινωνικής δικτύωσης, προκειμένου να καταγράψουμε τα κριτήρια με τα οποία οι περισσότεροι άνθρωποι επιλέγουν την οδοντόκρεμα που χρησιμοποιούν. Παράλληλα πραγματοποιήσαμε μέτρηση pH του σιέλου πριν και μετά τη χρήση οδοντόκρεμας, έτσι ώστε να εμπλουτίσουμε τη γνώση μας και εμπειρικά.
Παιχνιδοποίηση στην Εκπαίδευση
Authors: Κυριάκου Έλενα , Κλιάμπα Μελίνα , Σταυροπούλου Σταυροπούλου , Κορκολή Θεοφανία , Πριονιστής Γιάννης 
Published: December 2022

Η παιχνιδοποίηση (gamification) είναι μια σύγχρονη έννοια και αφορά τη χρήση μηχανισμών παιχνιδιού σε μη παιγνιώδεις καταστάσεις. Μέσω διάφορων τεχνικών που μιμούνται τα παιχνίδια, αποσκοπεί να βελτιώσει την μαθησιακή εμπειρία, να ενισχύσει τον βαθμό εμπλοκής με το μάθημα και να ενδυναμώσει την αίσθηση του σκοπού και της οικειότητας στις διάφορες διαδικασίες. Στην έρευνά μας δημιουργήσαμε ένα ερωτηματολόγιο για να εξετάσουμε τις αντιλήψεις των μαθητών σχετικά με την παιχνιδοποίηση στην εκπαίδευση. Στη συνέχεια, δημιουργήσαμε ένα εκπαιδευτικό παιχνίδι, το οποίο δοκίμασαν μαθητές του σχολείου μας και συλλέξαμε σχόλια ανατροφοδότησης από τους μαθητές.
ΝΕΕΣ ΜΟΡΦΕΣ ΕΝΕΡΓΕΙΑΣ: ΠΡΟΣ ΤΗΝ ΠΥΡΗΝΙΚΗ ΣΥΝΤΗΞΗ
Authors: Μπλάνας Σωκράτης , Παπαδογιάννης Έκτορας , Ποταμιάς Ηλίας 
Published: December 2022

Με τον πόλεμο να μαίνεται στην Ουκρανία και την ενεργειακή κρίση να κορυφώνεται ραγδαία, ανάγεται σε μείζων ζήτημα η ενεργειακή απεξάρτηση της Ευρώπης από τα ορυκτά καύσιμα και η ούτως ή άλλως περιβαλλοντικά απαραίτητη στροφή προς καθαρότερες πηγές ενέργειας. Στην παρούσα εργασία γίνεται αναφορά στο ενεργειακό προφίλ της Ελλάδας, στην μεγάλη - αν και ολοένα μειούμενη - εξάρτηση της χώρας μας από τον λιγνίτη, και στο αναγκαίο πέρασμα της στην μεταλιγνιτική εποχή. Από τις ανανεώσιμες πηγές ενέργειας δίνεται ιδιαίτερη έμφαση στην πυρηνική σύντηξη, διαδικασία «παραγωγής» καθαρής ενέργειας (που πραγματοποιείται ανεμπόδιστα στον ήλιο), με ελάχιστο περιβαλλοντικό αποτύπωμα, εξαιρετικά πλεονεκτήματα αλλά και μεγάλες δυσκολίες αξιοποίησής της στις συνθήκες της γης. Μέσα από μία μεγάλη πορεία δοκιμών, τα τελευταία επιτεύγματα ανάβουν το πράσινο φως και δημιουργούν υψηλές προσδοκίες για το άμεσο μέλλον.
Assessing and managing wound pain
Authors: Brown Annemarie 
Published: December 2022

Pain assessment and management is an important part of caring for people with wounds. Annemarie Brown provides an overview of the types of wound pain and strategies for minimising and managing it Different wounds produce different types of pain. Pain assessment should form part of the initial wound assessment and be reassessed regularly. Verbal cues and pain assessment tools are useful when assessing pain, and observing non-verbal cues, such as grimacing, guarding the affected area and limited movement can also be helpful. Nurses should stay up to date on how to minimise pain during dressing changes, as this has been shown to cause some of the highest levels of pain. Several types of analgesia can be used to manage wound pain, and the type prescribed should be based on the type of wound pain and level of pain experienced by the patient. It is key that health professionals always acknowledge how pain impacts their patient's quality of life.
Allergic rhinitis: classification, diagnosis and treatment
Authors: Salvador Laia Castro 
Published: December 2022

Allergic rhinitis is common in the UK. Laia Castro Salvador provides an overview of the classification, diagnosis and treatment of this condition Allergic rhinitis is the most common form of rhinitis. Allergic rhinitis symptoms can have a large impact on patients’ quality of life by affecting their sleep, social life and work/school performance. Allergic rhinitis can be classified depending on the frequency and severity of symptoms, and further divided into seasonal and perennial. Treatment for allergic rhinitis includes allergen avoidance, pharmacological therapies and patient education.
HISTÓRIA DA DISCIPLINA LÃNGUA PORTUGUESA EM MATO GROSSO DO SUL (1977-2008): QUESTÕES CONCEITUAIS
Authors: Bertoletti Estela Natalina Mantovani 
Published: December 2022

Este texto apresenta questões conceituais de pesquisa de pós-doutorado vinculada Ã UNESP/Araraquara, cujo tema é a história do ensino de língua portuguesa em Mato Grosso do Sul, relativamente ao conceito de currículo e história do currículo, à cultura escolar e Ã história das disciplinas escolares como campo de pesquisa. Trata-se de resultados parciais da análise que se fará sobre as normatizações empreendidas nos currículos de 1º grau/ensino fundamental prescritos para essa disciplina no estado em questão, desde 1977 . ano de criação do estado . até 2008 . ano de publicação do mais recente documento oficial para esse ensino no estado, para compreender sua história do ponto de vista oficial. Na pesquisa, concebe-se a escola dotada de uma cultura própria e as disciplinas, uma das produções ou criações próprias da escola.
HISTÓRIA DA EDUCAÇÃO MATEMÁTICA NOS ANOS INICIAIS: A PASSAGEM DO SIMPLES/COMPLEXO PARA O FÃCIL/DIFÃCIL
Authors: Valente Wagner Rodrigues 
Published: December 2022

O texto analisa as transformações dos processos didático-pedagógicos tendo em vista as propostas da chamada "pedagogia científica". Em específico, atém-se Ã quelas destinadas aos ensinos de matemática na escola primária na década de 1930. Toma como referência a obra de Lourenço Filho intitulada Introdução ao estudo da Escola Nova. Usa como instrumental teórico-metodológico aportes da História Cultural. Orienta-se pelo objetivo de historicizar a passagem do simples/complexo para a díade fácil/difícil.
A CONSTRUÇÃO DO CURRÃCULO DE SOCIOLOGIA NO COLÉGIO PEDRO II (1925-1941)
Authors: Soares Jefferson da Costa 
Published: December 2022

O artigo aborda a construção do currículo de Sociologia no período de 1925 a 1941, no Colégio Pedro II, primeira instituição a introduzir o ensino da disciplina em sua grade curricular. O principal referencial teórico adotado foi a tradição inglesa da História das Disciplinas Escolares ligada ao campo do Currículo, referenciada na obra de Ivor Goodson. A metodologia utilizada foi a análise documental de fontes do período estudado, dentre elas as existentes no Núcleo de Documentação e Memória do Colégio Pedro II (NUDOM), o texto das reformas educacionais, um relatório sobre o ensino de sociologia encaminhado Ã Presidência da República, e manuais didáticos elaborados por Delgado de Carvalho, primeiro catedrático efetivo da disciplina.