bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleeve gastrectomy decreases high-fat diet induced colonic pro-inflammatory status through the gut microbiota alterations
Authors: Cao Chong , Tan Xiaozhuo , Yan Hai , Shen Qiwei , Hua Rong , Shao Yikai , Yao Qiyuan 
Published: January 2023

BackgroundHigh-fat diet (HFD) induced obesity is characterized with chronic low-grade inflammation in various tissues and organs among which colon is the first to display pro-inflammatory features associated with alterations of the gut microbiota. Sleeve gastrectomy (SG) is currently one of the most effective treatments for obesity. Although studies reveal that SG results in decreased levels of inflammation in multiple tissues such as liver and adipose tissues, the effects of surgery on obesity related pro-inflammatory status in the colon and its relation to the microbial changes remain unknown.MethodsTo determine the effects of SG on the colonic pro-inflammatory condition and the gut microbiota, SG was performed on HFD-induced obese mice. To probe the causal relationship between alterations of the gut microbiota and improvements of pro-inflammatory status in the colon following SG, we applied broad-spectrum antibiotics cocktails on mice that received SG to disturb the gut microbial changes. The pro-inflammatory shifts in the colon were assessed based on morphology, macrophage infiltration and expressions of a variety of cytokine genes and tight junction protein genes. The gut microbiota alterations were analyzed using 16s rRNA sequencing. RNA sequencing of colon was conducted to further explore the role of the gut microbiota in amelioration of colonic pro-inflammation following SG at a transcriptional level.ResultsAlthough SG did not lead to pronounced changes of colonic morphology and macrophage infiltration in the colon, there were significant decreases in the expressions of several pro-inflammatory cytokines including interleukin-1β (IL-1β), IL-6, IL-18, and IL-23 as well as increased expressions of some tight junction proteins in the colon following SG, suggesting an improvement of pro-inflammatory status. This was accompanied by changing populations of the gut microbiota such as increased richness of Lactobacillus subspecies following SG. Importantly, oral administrations of broad-spectrum antibiotics to delete most intestinal bacteria abrogated surgical effects to relieve colonic pro-inflammation. This was further confirmed by transcriptional analysis of colon indicating that SG regulated inflammation related pathways in a manner that was gut microbiota relevant.ConclusionThese results support that SG decreases obesity related colonic pro-inflammatory status through the gut microbial alterations.
CytoSorb in patients with coronavirus disease 2019: A rapid evidence review and meta-analysis
Authors: Wei Shilin , Zhang Yanchun , Zhai Kerong , Li Jian , Li Mingming , Yang Jianbao , Zhang Rongzhi , Li Yongnan , Li Zhenzhen 
Published: January 2023

BackgroundAfter its approval by the European Union in 2011, CytoSorb therapy has been applied to control cytokine storm and lower the increased levels of cytokines and other inflammatory mediators in blood. However, the efficiency of this CytoSorb treatment in patients with coronavirus disease (COVID-19) still remains unclear. To elucidate the Cytosorb efficiency, we conducted a systematic review and single-arm proportion meta-analysis to combine all evidence available in the published literature to date, so that this comprehensive knowledge can guide clinical decision-making and future research.MethodsThe literature published within the period 1 December 2019 to 31 December 2021 and stored in the Cochrane Library, Embase, PubMed, and International Clinical Trials Registry Platform (ICTRP) was searched for all relevant studies including the cases where COVID-19 patients were treated with CytoSorb. We performed random-effects meta-analyses by R software (3.6.1) and used the Joanna Briggs Institute checklist to assess the risk of bias. Both categorical and continuous variables were presented with 95% confidence intervals (CIs) as pooled proportions for categorical variables and pooled means for continuous outcomes.ResultsWe included 14 studies with 241 COVID-19 patients treated with CytoSorb hemadsorption. Our findings reveal that for COVID-19 patients receiving CytoSorb treatment, the combined in-hospital mortality was 42.1% (95% CI 29.5–54.6%, I2 = 74%). The pooled incidence of adjunctive extracorporeal membrane oxygenation (ECMO) support was 73.2%. Both the C-reactive protein (CRP) and interleukin-6 (IL-6) levels decreased after CytoSorb treatment. The pooled mean of the CRP level decreased from 147.55 (95% CI 91.14–203.96) to 92.36 mg/L (95% CI 46.74–137.98), while that of IL-6 decreased from 339.49 (95% CI 164.35–514.63) to 168.83 pg/mL (95% CI 82.22–255.45).ConclusionsThe majority of the COVID-19 patients treated with CytoSorb received ECMO support. In-hospital mortality was 42.1% for the COVID-19 patients who had CytoSorb treatment. Both CRP and IL-6 levels decreased after Cytosorb treatment.
Patient-derived monoclonal antibodies to SARS-CoV-2 nucleocapsid protein N-terminal and C-terminal domains cross-react with their counterparts of SARS-CoV, but not other human betacoronaviruses
Authors: Wen Yingfen , Guo Wenjing , Min Yuyi , Zhong Kexin , Zhang Xulei , Xing Xiaomin , Tong Yuwei , Pan Yuejun , Hong Wenxin , Cai Weiping , Yu Lei 
Published: January 2023

IntroductionSARS-CoV-2 nucleocapsid (N) protein plays a key role in multiple stages of the viral life cycle such as viral replication and assembly. This protein is more conserved than the Spike protein of the virus and can induce both humoral and cell-mediated immune responses, thereby becoming a target for clinical diagnosis and vaccine development. However, the immunogenic characteristics of this protein during natural infection are still not completely understood.MethodsPatient-derived monoclonal antibodies (mAbs) against SARS-CoV-2 N protein were generated from memory B cells in the PBMCs using the antigen-specific B cell approach. For epitope mapping of the isolated hmAbs, a panel of series-truncated N proteins were used , which covered the N-terminal domain (NTD, aa 46-174 ) and C-terminal domain (CTD, aa 245-364 ), as well as the flanking regions of NTD and CTD. NTD- or CTD-specific Abs in the plasma from COVID-19 patients were also tested by ELISA method. Cross-binding of hmAbs or plasma Abs in COVID-19 patients to other human β-CoV N proteins was determined using the capture ELISA.ResultsWe isolated five N-specific monoclonal antibodies (mAbs) from memory B cells in the peripheral blood of two convalescent COVID-19 patients. Epitope mapping revealed that three of the patient-derived mAbs (N3, N5 and N31) targeted the C-terminal domain (CTD), whereas two of the mAbs (N83 and 3B7) targeted the N-terminal domain (NTD) of SARS-CoV-2 N protein. All five patient-derived mAbs were cross-reactive to the N protein of SARS-CoV but showed little to no cross-reactivity to the N proteins of other human beta coronaviruses (β-CoVs). We also tested 52 plasma samples collected from convalescent COVID-19 patients for Abs against the N proteins of human β-CoVs and found that 78.8% of plasma samples showed detectable Abs against the N proteins of SARS-CoV-2 and SARS-CoV. No plasma sample had cross-reactive Abs to the N protein of MERS-CoV. Cross-reactive Abs to the N proteins of OC43 and HKU1 were detected in 36.5% (19/52) and 19.2% (10/52) of plasma samples, respectively.DiscussionThese results suggest that natural SARS-CoV-2 infection elicits cross-reactive Abs to the N protein of SARS-CoV and that the five patient-derived mAbs to SARS-CoV-2 N protein NTD and CTD cross-react with their counterparts of SARS-CoV, but not other human β-CoVs. Thus, these five patient-derived mAbs can potentially be used for developing the next generation of COVID-19 At-Home Test kits for rapid and specific screening of SARS-CoV-2 infection.
End-of-neoadjuvant treatment circulating microRNAs and HER2-positive breast cancer patient prognosis: An exploratory analysis from NeoALTTO
Authors: Di Cosimo Serena , Ciniselli Chiara M. , Pizzamiglio Sara , Cappelletti Vera , Silvestri Marco , El-Abed Sarra , Izquierdo Miguel , Bajji Mohammed , Nuciforo Paolo , Huober Jens , Cameron David , Chia Stephen , Gomez Henry L. , Iorio Marilena V. , Vingiani Andrea , Pruneri Giancarlo , Verderio Paolo 
Published: January 2023

BackgroundThe absence of breast cancer cells in surgical specimens, i.e., pathological complete response (pCR), is widely recognized as a favorable prognostic factor after neoadjuvant therapy. In contrast, the presence of disease at surgery characterizes a prognostically heterogeneous group of patients. Here, we challenged circulating microRNAs (miRNAs) at the end of neoadjuvant therapy as potential prognostic biomarkers in the NeoALTTO study.MethodsPatients treated within the trastuzumab arm (i.e., pre-operative weekly trastuzumab for 6 weeks followed by the addition of weekly paclitaxel for 12 weeks; post-operative FEC for 3 cycles followed by trastuzumab up to complete 1 year of treatment) were randomized into a training (n= 54) and testing (n= 72) set. RT-PCR-based high-throughput miRNA profile was performed on plasma samples collected at the end of neoadjuvant treatment of both sets. After normalization, circulating miRNAs associated with event free survival (EFS) were identified by univariate and multivariate Cox regression model.ResultsStarting from 23 circulating miRNAs associated with EFS in the training set, we generated a 3-circulating miRNA prognostic signature consisting of miR-185-5p, miR-146a-5p, miR-22-3p, which was confirmed in the testing set. The 3-circulating miRNA signature showed a C-statistic of 0.62 (95% confidence interval [95%CI] 0.53-0.71) in the entire study cohort. By resorting to a multivariate Cox regression model we found a statistical significant interaction between the expression values of miR-194-5p and pCR status (p.interaction =0.005) with an estimate Hazard Ratio (HR) of 1.83 (95%CI 1.14- 2.95) in patients with pCR, and 0.87 (95%CI 0.69-1.10) in those without pCR. Notably, the model including this interaction along with the abovementioned 3-circulating miRNA signature provided the highest discriminatory capability with a C-statistic of 0.67 (95%CI 0.58-0.76).ConclusionsCirculating miRNAs are informative to identify patients with different prognosis among those with heterogeneous response after trastuzumab-based neoadjuvant treatment, and may be an exploitable tool to select candidates for salvage adjuvant therapy.
The diagnostic performance of radiomics-based MRI in predicting microvascular invasion in hepatocellular carcinoma: A meta-analysis
Authors: Liang Gao , Yu Wei , Liu Shuqin , Zhang Mingxing , Xie Mingguo , Liu Min , Liu Wenbin 
Published: January 2023

ObjectiveThe aim of this study was to assess the diagnostic performance of radiomics-based MRI in predicting microvascular invasion (MVI) in hepatocellular carcinoma (HCC).MethodThe databases of PubMed, Cochrane library, Embase, Web of Science, Ovid MEDLINE, Springer, and Science Direct were searched for original studies from their inception to 20 August 2022. The quality of each study included was assessed according to the Quality Assessment of Diagnostic Accuracy Studies 2 and the radiomics quality score. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated. The summary receiver operating characteristic (SROC) curve was plotted and the area under the curve (AUC) was calculated to evaluate the diagnostic accuracy. Sensitivity analysis and subgroup analysis were performed to explore the source of the heterogeneity. Deeks’ test was used to assess publication bias. ResultsA total of 15 studies involving 981 patients were included. The pooled sensitivity, specificity, PLR, NLR, DOR, and AUC were 0.79 (95%CI: 0.72–0.85), 0.81 (95%CI: 0.73–0.87), 4.1 (95%CI:2.9–5.9), 0.26 (95%CI: 0.19–0.35), 16 (95%CI: 9–28), and 0.87 (95%CI: 0.84–0.89), respectively. The results showed great heterogeneity among the included studies. Sensitivity analysis indicated that the results of this study were statistically reliable. The results of subgroup analysis showed that hepatocyte-specific contrast media (HSCM) had equivalent sensitivity and equivalent specificity compared to the other set. The least absolute shrinkage and selection operator method had high sensitivity and specificity than other methods, respectively. The investigated area of the region of interest had high specificity compared to the volume of interest. The imaging-to-surgery interval of 15 days had higher sensitivity and slightly low specificity than the others. Deeks’ test indicates that there was no publication bias (P=0.71).ConclusionRadiomics-based MRI has high accuracy in predicting MVI in HCC, and it can be considered as a non-invasive method for assessing MVI in HCC.
Lipid profile as a novel prognostic predictor for patients with acute myeloid leukemia
Authors: Bai Shenrui , Wang Huizhong , Shao Ruonan , Fu Bibo , Lu Shujing , Wang Jingzi , Lu Yue , Wang Hua 
Published: January 2023

PurposeThis study investigated the relationship between serum lipid levels and clinical outcomes in acute myeloid leukemia (AML) by establishing a predictive risk classification model.MethodA total of 214 AML patients who were pathologically diagnosed and treated with standard induction chemotherapy at Sun Yat-Sen University Cancer Center were included. The patients were randomly divided into the training (n = 107) and validation (n=107) cohorts. Univariate and multivariate Cox analyses were used to assess the value of triglyceride (TG), Apolipoprotein B (Apo B), Apo Apolipoprotein A-I (Apo A-I), cholesterol (CHO), and high-density lipoprotein (HDL) as prognostic factors for AML.ResultsAfter a series of data analyses, a five-factor model was established to divide the patients into high- and low-risk groups. Kaplan-Meier survival analysis showed that the high-risk group had a poor prognosis (P&lt;0.05). The area under the curve of the novel model for five-year OS was 0.737. A nomogram was constructed to integrate the model with age and the 2017 ELN cytogenetic classification, with the merged model showing improved accuracy with an area under the curve of 0.987 for five-year OS.ConclusionA novel model was constructed using a combination of the serum lipid profile and clinical characteristics of AML patients to enhance the predictive accuracy of clinical outcomes. The nomogram used the lipid profile which is routinely tested in clinical blood biochemistry and showed both specific prognostic and therapeutic potential.
Scleritis in rheumatoid arthritis: Before and during biologic era
Authors: Vergouwen Daphne P. C. , de Jong Pascal H. P. , Schreurs Marco W. J. , Berge Josianne C. Ten , Rothova Aniki 
Published: January 2023

ObjectivesScleritis represents a severe extra-articular manifestation of rheumatoid arthritis (RA). Recent clinical observations suggest a decreasing incidence of scleritis in RA, attributed to improved treatment options. Our study reports on the incidence and clinical characteristics of scleritis in RA observed in the biological era and reflects on our results in a historical perspective.MethodsWe performed a retrospective evaluation of all 1623 consecutive patients with RA diagnosed at the department of rheumatology between 2011 and 2021 at the Erasmus Medical Center to investigate the incidence of scleritis. We also reviewed clinical and laboratory data of all patients with scleritis and RA from the department of ophthalmology at our center. In addition, we reviewed the literature on this topic and discuss our results in view of changes over time.ResultsThe incidence of scleritis within recent series of patients with a diagnosis of RA in our tertiary center was 0,25% in 10 years (4 out of 1623; 2011-2021).The cumulative incidence of scleritis in RA based on literature review from the pre-biologic era varied from 0.7% per 8 years to 0,8% per 30 years. Manifestations and complications of scleritis remained unchanged over time, with scleral necrosis developing in more than 80% of cases and mortality of RA patients with scleritis remained similar to pre-biologic era (30% in 9 years after the onset of scleritis). The RA patients with scleritis often exhibited autoantibodies (rheumatoid factor and/or anti-citrullinated protein antibody) and erosive disease.ConclusionAlthough our recent series is characterized by a slightly lower incidence of scleritis in RA compared to the pre-biologic era, clinical presentation remained severe and similar to the pre-biologic era. Ophthalmologists and rheumatologists should be aware of scleritis as a severe extra-articular manifestation of RA.
The LIM Domain Protein BmFHL2 Inhibits Egg Production in Female Silkworm, Bombyx mori
Authors: Yuan Qian , Sun Xiaoning , Lu Riming , Qu Zhigang , Ding Xueyan , Dai Taiming , Qiu Jianfeng , Tan Yumei , Zhu Ruihong , Pan Zhonghua , Xu Shiqing , Sima Yanghu 
Published: January 2023

The female Bombyx mori accumulates a large amount of egg proteins, mainly Vg and 30K, during egg formation to provide nutrition for embryo development. The synthesis and transport of Vg have been extensively studied, particularly the regulation of Vg transcription induced by 20E; however, the mechanism of 30K protein synthesis is poorly studied. As a model organism of the order Lepidoptera, B. mori has high reproduction potential. In the present study, we found that the FHL2 homologous gene (BmFhl2) in B. mori is involved in inhibiting female egg formation by influencing the synthesis of 30K protein. Interference of BmFhl2 expression in silkworm females increased 30K protein synthesis, accelerated ovarian development, and significantly increased the number of eggs produced and laid; however, the 20E pathway was inhibited. The transcription levels of Vg and 30Kc19 were significantly downregulated following BmFhl2 overexpression in the silkworm ovarian cell line BmN. The Co-IP assay showed that the potential binding protein of BmFHL2 included three types of 30K proteins (30Kc12, 30Kc19, and 30Kc21). These results indicate that BmFHL2 participates in egg formation by affecting 30K protein in female B. mori.
Considerations regarding the macrobotanical remains from ceramic vessels found at Șoimuș – La Avicola (Ferma 2), Hunedoara County
Authors: Golea Mihaela 
Published: January 2023

In 2011, a joint team from various cultural institutions (“Vasile Pârvan” Institute of Archaeology, Museum of Dacian and Roman Civilisation and National Romanian History Museum) have collaborated in rescue excavations on the A1 highway (Lugoj– Deva). This research uncovered a multi‑stratified settlement with occupations dated to the Neolithic, Bronze Age, Iron Age, Roman and Medieval periods. The Neolithic period (Vinča culture) is represented by two main layers. Radiocarbon dated the Neolithic occupation between the end of the sixth millennium and the beginning of the fifth millennium BC (Vinča culture, B and C phases). It this study, the contents of twenty‑one complete and fragmented ceramic pots were analysed. The infill sediment from these vessels was wet sieved. The soil infill of eleven vessels yielded macrobotanical remains, mainly charred seeds. The context of these pots are as follows : five vessels were discovered in pits, two were found in dwellings, three came from the occupational layers, and one from an unknown context. The archaeobotanical samples recovered consist of fragments of caryopses, chaff of wheat (Triticum sp.) and einkorn (Triticum monococcum)
and some seeds of weeds and shrubs.
EKSPLORASI EFEKTIVITAS E-TRAINING TERHADAP PENINGKATAN ORGANIZATIONAL PERFORMANCE PT JAKARTA INTERNATIONAL HOTELS DEVELOPMENT (JIHD)
Authors: Happy Vanesa Valentina , Wibowo Agus , Wardjono Gunarto , Wardjono Gunarto , Yudi Widiyarto Ervan , Indiyah Kus 
Published: January 2023

<p align="justify">Pandemi COVID-19 membawa dampak pada semua sendi kehidupan, salah satu yang terdampak parah adalah sektor perekonomian dan industri. Demi menjaga produktifitas ditengah pandemi COVID-19 dan bertahan memenangkan karena persaingan yang ketat ditengah ketidaktentuan, perusahaan tertantang untuk mencapai kinerja optimal.Penelitian ini bertujuan untuk menganalisis Efektifitas <em>E-Training</em> terhadap <em>Organizational Performance</em> yang ditinjau juga melalui <em>Employee Motivation</em> dan <em>Employee Job Satisfation</em>. Penelitian ini menggunakan metode kuantitatif dengan menggunakan <em>Structural Equation Modelling </em>(SEM) dengan jumlah responden sebesar 284 karyawan PT Jakarta International Hotels &amp; Development.Hasil penelitian ini menunjukan bahwa <em>e-training </em>merupakan faktor yang terbukti dapat meningkatkan <em>job satisfaction </em>dan <em>employee motivation</em>. Penggunaan e-training dalam proses pengembangan karyawan perlu untuk terus dilakukan. Hasil penelitian ini juga memberikan informasi data yang dapat dianalisis oleh perusahaan untuk mencari tahu hal-hal yang merupakan kebutuhan dan keinginan dari karyawan dan berupaya memenuhi kebutuhan karyawan, seperti gaji yang layak, bonus sesuai kinerja dan juga dukungan lainnya sehingga kepuasan dari karyawan akan terus meningkat. Motivasi karyawan juga merupakan faktor penting yang perlu ditingkatkan karena terbukti bahwa motivasi merupakan faktor yang dapat meningkatkan kinerja karyawan. Perusahaan perlu untuk terus meningkatkan motivasi karyawan dengan cara memberikan dukungan yang baik dan juga terus menciptakan kepuasan kerja yang lebih baik.</p><p> </p><strong>Kata kunci</strong>: <em>E-training, employee motivation, employee satisfaction, organizational performance, Structural Equation Modelling (SEM)</em>
PENGEMBANGAN MEDIA PEMBELAJARAN BERBASIS ARTICULATE STORYLINE 3 MATERI GAYA PADA SISWA KELAS IV SDN 009 SUNGAI KUNJANG
Authors: Suhartini Erna , Ayu Wandini Intan , Ramli Buhari Muhammad 
Published: January 2023

Penelitian ini dilatarbelakangi oleh kebutuhan pendidikan terhadap media pembelajaran yang mengimbangi kemajuan zaman. Penelitian ini bertujuan untuk mengembangkan sebuah media pembelajaran berbasis Articulate Storyline 3 materi gaya pada siswa kelas IV di SDN 009 Sungai Kunjang. Metode yang diterapkan dalam penelitian ini menggunakan metode Research and Development (R&D) dengan prosedur pengembangan model Borg and Gall yang dimodifikasi peneliti menjadi 5 tahapan yaitu meliputi tahap pengumpulan informasi, pengembangan produk awal, validasi produk, ujicoba, dan penyempurnaan produk. Teknik pengumpulan data kuesioner digunakan dalam penelitian ini adalah yang berupa analisis kebutuhan, penilaian kelayakan media, dan respon peserta didik. Hasil dari penelitian ini adalah media pembelajaran berbasis Articulate Storyline yang telah dilakukan penilaian kelayakan mendapatkan rata-rata persentase oleh ahli media sebesar 80,5% yang berarti dalam kategori “tinggi”, mendapatkan rata-rata persentase oleh ahli materi sebesar 96,875% yang berarti dalam kategori “sangat tinggi”, dan dari hasil kuesioner respon peserta didik sebanyak 28 peserta didik dengan ujicoba kelompok kecil mendapatkan hasil respon positif hasil rata-rata persentase 94,92% dan kelompok besar mendapatkan hasil rata-rata persentase 98,10% yang keduanya berarti dalam kategori “sangat tinggi”.