bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural evidence for intermediates during O2 formation in photosystem II
Authors: Bhowmick Asmit , Hussein Rana , Bogacz Isabel , Simon Philipp S. , Ibrahim Mohamed , Chatterjee Ruchira , Doyle Margaret D. , Cheah Mun Hon , Fransson Thomas , Chernev Petko , Kim In-Sik , Makita Hiroki , Dasgupta Medhanjali , Kaminsky Corey J. , Zhang Miao , Gätcke Julia , Haupt Stephanie , Nangca Isabela I. , Keable Stephen M. , Aydin A. Orkun , Tono Kensuke , Owada Shigeki , Gee Leland B. , Fuller Franklin D. , Batyuk Alexander , Alonso-Mori Roberto , Holton James M. , Paley Daniel W. , Moriarty Nigel W. , Mamedov Fikret , Adams Paul D. , Brewster Aaron S. , Dobbek Holger , Sauter Nicholas K. , Bergmann Uwe , Zouni Athina , Messinger Johannes , Kern Jan , Yano Junko , Yachandra Vittal K. 
Published: May 2023

AbstractIn natural photosynthesis, the light-driven splitting of water into electrons, protons and molecular oxygen forms the first step of the solar-to-chemical energy conversion process. The reaction takes place in photosystem II, where the Mn4CaO5 cluster first stores four oxidizing equivalents, the S0 to S4 intermediate states in the Kok cycle, sequentially generated by photochemical charge separations in the reaction center and then catalyzes the O–O bond formation chemistry1–3. Here, we report room temperature snapshots by serial femtosecond X-ray crystallography to provide structural insights into the final reaction step of Kok’s photosynthetic water oxidation cycle, the S3→[S4]→S0 transition where O2 is formed and Kok’s water oxidation clock is reset. Our data reveal a complex sequence of events, which occur over micro- to milliseconds, comprising changes at the Mn4CaO5 cluster, its ligands and water pathways as well as controlled proton release through the hydrogen-bonding network of the Cl1 channel. Importantly, the extra O atom Ox, which was introduced as a bridging ligand between Ca and Mn1 during the S2→S3 transition4–6, disappears or relocates in parallel with Yz reduction starting at approximately 700 μs after the third flash. The onset of O2 evolution, as indicated by the shortening of the Mn1–Mn4 distance, occurs at around 1,200 μs, signifying the presence of a reduced intermediate, possibly a bound peroxide.
A dataset of direct observations of sea ice drift and waves in ice
Authors: Rabault Jean , Müller Malte , Voermans Joey , Brazhnikov Dmitry , Turnbull Ian , Marchenko Aleksey , Biuw Martin , Nose Takehiko , Waseda Takuji , Johansson Malin , Breivik Øyvind , Sutherland Graig , Hole Lars Robert , Johnson Mark , Jensen Atle , Gundersen Olav , Kristoffersen Yngve , Babanin Alexander , Tedesco Paulina , Christensen Kai Haakon , Kristiansen Martin , Hope Gaute , Kodaira Tsubasa , de Aguiar Victor , Taelman Catherine , Quigley Cornelius P. , Filchuk Kirill , Mahoney Andrew R 
Published: May 2023

AbstractVariability in sea ice conditions, combined with strong couplings to the atmosphere and the ocean, lead to a broad range of complex sea ice dynamics. Morein-situmeasurements are needed to better identify the phenomena and mechanisms that govern sea ice growth, drift, and breakup. To this end, we have gathered a dataset ofin-situobservations of sea ice drift and waves in ice. A total of 15 deployments were performed over a period of 5 years in both the Arctic and Antarctic, involving 72 instruments. These provide both GPS drift tracks, and measurements of waves in ice. The data can, in turn, be used for tuning sea ice drift models, investigating waves damping by sea ice, and helping calibrate other sea ice measurement techniques, such as satellite based observations.
Intracerebral hemorrhage CT scan image segmentation with HarDNet based transformer
Authors: Piao Zhegao , Gu Yeong Hyeon , Jin Hailin , Yoo Seong Joon 
Published: May 2023

AbstractAlthough previous studies conducted on the segmentation of hemorrhage images were based on the U-Net model, which comprises an encoder-decoder architecture, these models exhibit low parameter passing efficiency between the encoder and decoder, large model size, and slow speed. Therefore, to overcome these drawbacks, this study proposes TransHarDNet, an image segmentation model for the diagnosis of intracerebral hemorrhage in CT scan images of the brain. In this model, the HarDNet block is applied to the U-Net architecture, and the encoder and decoder are connected using a transformer block. As a result, the network complexity was reduced and the inference speed improved while maintaining the high performance compared to conventional models. Furthermore, the superiority of the proposed model was verified by using 82,636 CT scan images showing five different types of hemorrhages to train and test the model. Experimental results showed that the proposed model exhibited a Dice coefficient and IoU of 0.712 and 0.597, respectively, in a test set comprising 1200 images of hemorrhage, indicating better performance compared to typical segmentation models such as U-Net, U-Net++, SegNet, PSPNet, and HarDNet. Moreover, the inference time was 30.78 frames per second (FPS), which was faster than all en-coder-decoder-based models except HarDNet.
Feasibility of in vivo swine models using guide wire-assisted intraductal radiofrequency ablation for benign biliary stricture
Authors: Park Jae Keun , Yang Ju-Il , Park Joo Kyung , Lee Kwang Hyuck , Lee Jong Kyun , Lee Kyu Taek 
Published: May 2023

AbstractSeveral in vivo swine models of benign biliary stenosis (BBS) have been recently reported for preclinical studies of novel endoscopic techniques and devices. The aim of this study was to evaluate the efficacy and feasibility of large animal models of BBS by using intraductal radiofrequency ablation (RFA) assisted by guide wire. Six in vivo swine models were made by using an intraductal RFA for cauterization at 10 W, 80 °C, 90 s in the common bile duct (CBD). Endoscopic retrograde cholangiopancreatography (ERCP) was performed with cholangiography and histologic evaluation was done for the common bile duct. Blood tests were examined before, after, and at the final follow-up. Guide wire assisted RFA electrode produced BBS in all (6/6, 100%) animal models without severe complications. Fluoroscopy findings at 2 weeks after intraductal RFA in every model revealed BBS in the common bile duct. In histologic evaluations, fibrosis and chronic inflammatory changes were noted. After the procedure, ALP, GGT, and CRP were elevated and decreased after an appropriate drain. A swine model of BBS is developed by inducing intraductal thermal injury using intraductal RFA assisted by guide wire. This novel technique for inducing BBS in swine is effective and feasible.
Eyestalk transcriptome and methyl farnesoate titers provide insight into the physiological changes in the male snow crab, Chionoecetes opilio, after its terminal molt
Authors: Toyota Kenji , Yamamoto Takeo , Mori Tomoko , Mekuchi Miyuki , Miyagawa Shinichi , Ihara Masaru , Shigenobu Shuji , Ohira Tsuyoshi 
Published: May 2023

AbstractThe snow crab, Chionoecetes opilio, is a giant deep-sea brachyuran. While several decapod crustaceans generally continue to molt and grow throughout their lifetime, the snow crab has a fixed number of molts. Adolescent males continue to molt proportionately to their previous size until the terminal molt at which time an allometric increase in chela size occurs and an alteration of behavioral activities occurs, ensuring breeding success. In this study, we investigated the circulating concentrations of methyl farnesoate (an innate juvenile hormone in decapods) (MF) before or after the terminal molt in males. We then conducted eyestalk RNAseq to obtain molecular insight into the regulation of physiological changes after the terminal molt. Our analyses revealed an increase in MF titers after the terminal molt. This MF surge may be caused by suppression of the genes that encode MF-degrading enzymes and mandibular organ-inhibiting hormone that negatively regulates MF biosynthesis. Moreover, our data suggests that behavioral changes after the terminal molt may be driven by the activation of biogenic amine-related pathways. These results are important not only for elucidating the physiological functions of MFs in decapod crustaceans, which are still largely unknown, but also for understanding the reproductive biology of the snow crab.
Magnetostatic interaction between Bloch point nanospheres
Authors: Zambrano-Rabanal Cristobal , Valderrama Boris , Tejo Felipe , Elías Ricardo Gabriel , Nunez Alvaro S. , Carvalho-Santos Vagson L. , Vidal-Silva Nicolás 
Published: May 2023

AbstractThree-dimensional topological textures have become a topic of intense interest in recent years. This work uses analytical and numerical calculations to determine the magnetostatic field produced by a Bloch point (BP) singularity confined in a magnetic nanosphere. It is observed that BPs hosted in a nanosphere generate magnetic fields with quadrupolar nature. This finding is interesting because it shows the possibility of obtaining quadrupole magnetic fields with just one magnetic particle, unlike other propositions considering arrays of magnetic elements to generate this kind of field. The obtained magnetostatic field allows us to determine the interaction between two BPs as a function of the relative orientation of their polarities and the distance between them. It is shown that depending on the rotation of one BP related to the other, the magnetostatic interaction varies in strength and character, being attractive or repulsive. The obtained results reveal that the BP interaction has a complex behavior beyond topological charge-mediated interaction.
A 6-year nationwide population-based study on the current status of gastric endoscopic resection in Korea using administrative data
Authors: Park Jae Yong , Kim Mi-Sook , Kim Beom Jin , Kim Jae Gyu 
Published: May 2023

AbstractGastric endoscopic resection (ER) is widely performed in Korea. This study aimed to investigate the overall status of gastric ER in Korea. We enrolled ESD or EMR cases performed for gastric cancer and adenoma from 2012 to 2017 by searching the NHIS database. The annual trend of gastric ER and the clinical characteristics were investigated. Institutions were classified into very high-, high-, low-, and very low volume centers (VHVC, HVC, LVC, and VLVC) by the procedure numbers, and institutional types, regional distributions, and medical resources were investigated accordingly. There were 175,370 ER cases during the study period, with an increasing trend over time. The average annual ESD procedure numbers were 3.9, 54.5, 249.5, and 540.3 cases in 131 VLVCs, 119 LVCs, 24 HVCs, and 12 VHVCs, respectively. Among ESD-performing institutions, 44.8% were located in the Seoul Capital Area. The distribution of medical resources showed a positive correlation with the procedural volume. Similar tendencies were also demonstrated in EMR, with some differences in hospital types and regional distribution. Gastric ER and ESD are increasing in Korea. There was a significant variance in the number of ER procedures and the distribution of types, regions, and medical resources according to the procedural volume.
Effect of target thickness and laser irradiance on the back-reflection-enhanced laser-induced breakdown spectroscopy signal in glass
Authors: Elhassan Asmaa , Abdel-Harith Mohamed , Abdelhamid Mahmoud 
Published: May 2023

AbstractIn the work that is being presented here, the effect of sample thickness and laser irradiance on the reduction of the signal-to-background ratio SBG and the plasma parameters, specifically electron temperature and electron density, is being investigated using back-reflection-enhanced laser-induced breakdown spectroscopy (BRELIBS). Copper and silver discs that had been highly polished were attached to the back surface of the glass target, and the Nd-YAG laser beam that was focused on the front surface of the target was tuned to its fundamental wavelength. The thicknesses of the transparent glass samples that were analysed were 1 mm, 3 mm, and 6 mm. One is able to achieve a range of different laser irradiance levels by adjusting the working distance that exists between the target sample and the focusing lens. The end result of this is that the signal-to-background ratio in the BRELIBS spectra of thicker glass samples is significantly lower as compared to the ratio in the spectra of thinner glass samples. In addition, a significant influence of modifying the laser irradiance (by increasing the working distance on the SBG ratio) is seen at various glass thicknesses for both BRELIBS and LIBS, with BRELIBS having a better SBG. Nevertheless, the laser-induced plasma parameter known as the electron temperature has not been significantly impacted by the decrease in the glass thickness.
Blink rate and facial orientation reveal distinctive patterns of attentional engagement in autistic toddlers: a digital phenotyping approach
Authors: Krishnappa Babu Pradeep Raj , Aikat Vikram , Di Martino J. Matias , Chang Zhuoqing , Perochon Sam , Espinosa Steven , Aiello Rachel , L. H. Carpenter Kimberly , Compton Scott , Davis Naomi , Eichner Brian , Flowers Jacqueline , Franz Lauren , Dawson Geraldine , Sapiro Guillermo 
Published: May 2023

AbstractDifferences in social attention are well-documented in autistic individuals, representing one of the earliest signs of autism. Spontaneous blink rate has been used to index attentional engagement, with lower blink rates reflecting increased engagement. We evaluated novel methods using computer vision analysis (CVA) for automatically quantifying patterns of attentional engagement in young autistic children, based on facial orientation and blink rate, which were captured via mobile devices. Participants were 474 children (17–36 months old), 43 of whom were diagnosed with autism. Movies containing social or nonsocial content were presented via an iPad app, and simultaneously, the device’s camera recorded the children’s behavior while they watched the movies. CVA was used to extract the duration of time the child oriented towards the screen and their blink rate as indices of attentional engagement. Overall, autistic children spent less time facing the screen and had a higher mean blink rate compared to neurotypical children. Neurotypical children faced the screen more often and blinked at a lower rate during the social movies compared to the nonsocial movies. In contrast, autistic children faced the screen less often during social movies than during nonsocial movies and showed no differential blink rate to social versus nonsocial movies.
Management and outcome trends in type 2 myocardial infarction: an investigation from the SWEDEHEART registry
Authors: Eggers K. M. , Baron T. , Chapman A. R. , Gard A. , Lindahl B. 
Published: May 2023

AbstractDespite poor prognosis, patients with type 2 myocardial infarction (MI) tend to be underdiagnosed and undertreated compared to those with type 1 MI. Whether this discrepancy has improved over time is uncertain. We conducted a registry-based cohort study investigating type 2 MI patients managed at Swedish coronary care units (n = 14,833) during 2010–2022. Multivariable-adjusted changes (first three vs last three calendar years of the observation period) were assessed regarding diagnostic examinations (echocardiography, coronary assessment), provision of cardioprotective medications (betablockers, renin–angiotensin–aldosterone-system inhibitors, statins) and 1-year all-cause mortality. Compared to type 1 MI patients (n = 184,329), those with type 2 MI less often had diagnostic examinations and cardioprotective medications. Increases in the use of echocardiography (OR 1.08 [95% confidence interval 1.06–1.09]) and coronary assessment (OR 1.06 [95% confidence interval 1.04–1.08]) were smaller compared to type 1 MI (pinteraction < 0.001). The provision of medications did not increase in type 2 MI. All-cause mortality rate in type 2 MI was 25.4% without temporal change (OR 1.03 [95% confidence interval 0.98–1.07]). Taken together, the provision of medications and all-cause mortality did ot improve in type 2 MI despite modest increases in diagnostic procedures. This emphasizes the need of defining optimal care pathways in these patients.
Effects of total daily light integral from blue and broad-band red LEDs on flowering of saffron (Crocus sativus L.)
Authors: Gao Dan , Ji Xinyu , Yuan Qing , Pei Weizhong , Zhang Xue , Li Fusheng , Han Qiuyi , Zhang Shanduan 
Published: May 2023

AbstractPresent indoor cultivation of saffron (Crocus sativus L.) only depends on artificial planting experience, so that flower number and stigma yield are seriously affected in case of cloudy or rainy days and temperature changes. In this study, a luminaire was used at 10-h photoperiod combined 450 nm blue LEDs with 660 nm broad-band red LEDs, which respectively had full width at half maximum (FWHM) of 15 nm and 85 nm, in a ratio of blue: red: far-red light = 20%: 62%: 18%. The influence of total daily light integral (TDLI) was evaluated on flowering characteristics, stigma quality, as well as leaf morphological characteristics. The results showed that flower number, daily flowering proportion, stigma dry weight and crocetin esters content were significantly correlated with TDLI (P < 0.01). The increasing TDLI could slightly promote leaf width and leaf area beyond buds, but had no significant effect on bud length and leaf length. Both the average flower number per corm and dried stigma yield was the highest under the 150 mol m−2 TDLI treatment, up to 3.63 and 24.19 mg respectively. The former was 0.7 more than that under original natural light treatment, while the later was 50% higher. Totaling, combining blue LEDs with a broad-band red LEDs of the 150 mol m−2 TDLI was the most favorable condition for flower number and stigma quality of saffron in this study.
Real world evidence of adjuvant trastuzumab in HER2 positive early breast cancer
Authors: Lluch-Gómez J. , Núñez-Álvarez V. , de la Torre-Hita C. , Bernal-Gómez M. , Campini-Bermejo A. , Perdomo-Zaldívar E. , Rodríguez-Pérez L. , Calvete-Candenas J. , Martínez-Bautista M. J. , Benítez-Rodríguez E. , Baena-Cañada J. M. 
Published: May 2023

AbstractAdjuvant trastuzumab in HER2+ breast cancer reduces recurrence and mortality, and has been the standard treatment since 2006. The objective was to analyze health outcomes in the real world. Observational, retrospective study of patients with HER2+ breast cancer, stages I–III, treated with adjuvant trastuzumab in the past 15 years in only one center and for the first time in Spain. Survival was analyzed according to the number of cycles and cardiotoxicity. Two hundred and seventy-five HER2positive patients (18.60%) out of 1479 received adjuvant (73%) or neoadjuvant/adjuvant (26%) trastuzumab, concomitantly (90%) or sequentially (10%) with chemotherapy. The probability of overall and disease-free survival (OS and DFS) at 5 years was 0.93 (95% CI 0.89–0.96), and 0.88 (95% CI 0.83–0.92). The number of cases with a significant and asymptomatic decrease in ventricular ejection fraction and heart failure were 54 (19.64%) and 12 (4.36%), respectively. Sixty-eight patients (24.70%) received 16 or fewer cycles, especially those older than 65 (OR 0.371, 95% CI 0.152–0.903; p = 0.029) and with cardiotoxicity (OR 15.02, 95% CI 7.437–30.335; p < 0.001). The risk of cardiotoxicity was associated with having received radiotherapy (OR 0.0362, 95% CI 0.139–0.938; p = 0.037). Arterial hypertension (HR 0.361, 95% CI 0.151–0.863, p = 0.022), neoadjuvant treatment (HR 0.314, 95% CI 0.132–0.750, p = 0.009) and cardiotoxicity (HR 2.755, 95% CI 1.235–6.143, p = 0.013) maintained significant association with OS. Only neoadjuvant treatment maintained a significant association with DFS (HR 0.437, 95% CI 0.213–0.899, p = 0.024). The effectiveness of neoadjuvant and adjuvant trastuzumab can be considered comparable to those of clinical trials. In the real world, factors such as age, hypertension, radiotherapy, neoadjuvant treatment, and cardiotoxicity should be taken into consideration to optimize outcomes.
Impact of anti-thymocyte globulin on survival outcomes in female-to-male allogeneic hematopoietic stem cell transplantation
Authors: Tamaki Masaharu , Akahoshi Yu , Ashizawa Masahiro , Misaki Yukiko , Koi Satoshi , Kim Sung-Won , Ozawa Yukiyasu , Fujiwara Shin-ichiro , Kako Shinichi , Matsuoka Ken-ichi , Sawa Masashi , Katayama Yuta , Onizuka Makoto , Kanda Yoshinobu , Fukuda Takahiro , Atsuta Yoshiko , Yakushijin Kimikazu , Nakasone Hideki 
Published: May 2023

AbstractAllogeneic hematopoietic cell transplantation between female donors and male recipients (female-to-male allo-HCT) is a well-established risk factor for inferior survival outcomes due to a higher incidence of graft-versus-host disease (GVHD). However, a clinical significance of anti-thymocyte globulin (ATG) in the female-to-male allo-HCT has not been elucidated. In this study, we retrospectively evaluated male patients who underwent allo-HCT between 2012 and 2019 in Japan. In the female-to-male allo-HCT cohort (n = 828), the use of ATG was not associated with a decreased risk of GVHD (HR of acute GVHD 0.691 [95% CI: 0.461–1.04], P = 0.074; HR of chronic GVHD 1.06 [95% CI: 0.738–1.52], P = 0.76), but was associated with favorable overall survival (OS) and a decreased risk of non-relapse mortality (NRM) (HR of OS 0.603 [95% CI: 0.400–0.909], P = 0.016; HR of NRM 0.506 [95% CI: 0.300–0.856], P = 0.011). The use of ATG in female-to-male allo-HCT resulted in survival outcomes that were almost equivalent to those in the male-to-male allo-HCT group. Therefore, GVHD prophylaxis with ATG might overcome the inferiority of survival outcomes in female-to-male allo-HCT.
Efficiency and consistency enhancement for alkaline electrolyzers driven by renewable energy sources
Authors: Xia Yanghong , Cheng Haoran , He Hanghang , Wei Wei 
Published: May 2023

AbstractLow-cost alkaline water electrolysis from renewable energy sources (RESs) is suitable for large-scale hydrogen production. However, fluctuating RESs lead to poor performance of alkaline water electrolyzers (AWEs) at low loads. Here we explore two urgent performance issues: inefficiency and inconsistency. Through detailed operation process analysis of AWEs and the established equivalent electrical model, we reveal the mechanisms of inefficiency and inconsistency of low-load AWEs are related to the physical structure and electrical characteristics. Furthermore, we propose a multi-mode self-optimization electrolysis converting strategy to improve the efficiency and consistency of AWEs. In particular, compared to a conventional dc power supply, we demonstrate using a lab-scale and large-scale commercially available AWE that the maximum efficiency can be doubled while the operation range of the electrolyzer can be extended from 30–100% to 10–100% of rated load. Our method can be easily generalized and can facilitate hydrogen production from RESs.
Hybridizing research and decision-making as a path toward sustainability in marine spaces
Authors: Boemare C. , Mosseri E. , Agin G. , Bramanti L. , Certain R. , Claudet J. , Guizien K. , Jabouin C. , Lagurgue X. , Lenfant P. , Levrel H. , Michel C. , Musard O. , Verdoit-Jarraya M. 
Published: May 2023

AbstractProjecting the combined effect of management options and the evolving climate is necessary to inform shared sustainable futures for marine activities and biodiversity. However, engaging multisectoral stakeholders in biodiversity-use scenario analysis remains a challenge. Using a French Mediterranean marine protected area (MPA) as a marine social-ecological case study, we coupled codesigned visioning narratives at horizon 2050 with an ecosystem-based model. Our analysis revealed a mismatch between the stated vision endpoints at 2050 and the model prediction narrative objectives. However, the discussions that arose from the approach opened the way for previously unidentified transformative pathways. Hybridizing research and decision-making with iterative collaborative modeling frameworks can enhance adaptive management policies, leveraging pathways toward sustainability.