bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microvascular endothelial function assessed using peripheral arterial tonometry in adolescents with repaired congenital heart disease
Authors: Odanaka Yutaka , Kishi Kanta , Takigiku Kiyohiro , Ashida Atsuko , Ozaki Noriyasu , Ashida Akira 
Published: May 2023

Abstract
Atherosclerosis can develop in adult patients with congenital heart disease (CHD) and should be given attention. Endothelial function is well known as a predictor of the development of atherosclerosis but has not been well investigated in patients with repaired CHD. This study aimed to clarify the endothelial function and its relationship with clinical backgrounds and parameters in adolescents with various types of repaired CHD. Endothelial function was evaluated using peripheral arterial tonometry (PAT). The reactive hyperemia index (RHI) was evaluated and compared between adolescents with repaired CHD and those in the control group. The relationship between the clinical background and parameters was also investigated in patients with repaired CHD. Forty-eight patients with repaired CHD (age 14.0 ± 3.3 years) and 114 healthy volunteers were included in this study. The patients with repaired CHD comprised 16 with repaired non-cyanotic CHD, 14 with repaired tetralogy of Fallot, and 18 who underwent Fontan procedure. RHI in the repaired CHD group was significantly lower than in the control group. There was no significant correlation between the RHI and biochemical blood markers, such as uric acid, creatine, and brain natriuretic peptide levels. The RHI was significantly higher in patients taking angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers (ARBs) than in those who did not. Endothelial function was impaired in adolescents with repaired CHD compared to that in the control group. Microvascular endothelial dysfunction developed even in adolescents with simple non-cyanotic CHD.
Electric Vehicle Charging Load Optimization Strategy Based on Dynamic Time-of-Use Tariff
Authors: Zhong Shuwei , Che Yanbo , Zhang Shangyuan 
Published: May 2023

Abstract
A new electricity price mechanism is proposed in this paper, namely dynamic time-of-use tariff, to address the problem that the static time-of-use tariff cannot be dynamically adjusted with the actual operating state of the grid and is prone to generate new load peaks by concentrated charging of electric vehicles (EVs) in the valley hours. Dynamic time-of-use tariff reclassifies the peak -valley hours with the fuzzy C-mean (FCM) clustering algorithm, and then dynamically adjusts the peak and valley tariff according to the actual load of each hour. Based on the dynamic time-of-use tariff, an EV charging optimization model is established with the objective function of minimum EV users charging cost and minimum grid load variance, and its constraints include user-side constraints and charging station-side constraints. In order to solve the multi-objective optimization problem, a weight selection principle with equal loss rate of the two objectives is proposed to transform the multi-objective optimization problem into a single-objective optimization problem, and finally the mathematical solver GUROBI is invoked to solve the model. The results show that the EV charging load optimization strategy based on dynamic time-of-use tariff can better balance the interests between charging stations and EV users under different number and ratio of EVs connected to the grid, and can effectively reduce the grid load variance.
Relationships Between Physical Parameters ofUmbral Dots Measured for 12 Sunspot Umbras withThe Goode Solar Telescope
Authors: CALISIR M. Ali , YAZICI H. Tayfun , KILCIK Ali , YURCHYSHYN Vasyl 
Published: May 2023

Abstract
We present a comprehensive analysis of the physical parameters and relationships of umbral dots (UDs), which assists in our understanding of the physical properties of the Sun. This study is based on a detailed analysis of UDs detected in 12 umbras belonging to 10 different sunspots using high-resolution data recorded by the Goode Solar Telescope at the Big Bear Solar Observatory. We obtained the physical parameters (brightness, diameter, eccentricity, lifetime, dynamic velocity) of each UD and calculated correlation coefficients using linear and nonlinear approaches to reveal the relationships between these parameters. We found that: i) the diameter of UDs are varying between 92.2 km to 246.5 km, the eccentricity varies between 0.02 to 0.65, the lifetime of UDs vary from 0.75 to 120.00 min and the dynamic velocities are varying from 0.01 km/s to 3.80 km/s. ii) The intensity-diameter and diameter-eccentricity show the highest degree of correlation, while the lowest linear correlation was obtained for the diameter-lifetime and the lowest nonlinear correlation was obtained for the eccentricity-lifetime relationships. iii) In general the nonlinear correlation coefficients are higher than the linear correlation without any exception. iv) The linear and nonlinear correlation coefficients are very close to each other in the case of diameter-eccentricity relation. v) While the average diameter, intensity, and eccentricity are related to the umbral area, the average lifetime and dynamic velocity of UDs are not dependent on the umbral area.
Molecular and biochemical response of Cannabis sativa plant to cadmium stress and the role of molybdenum
Authors: Ali Nasir , Hadi Fazal , Ali Mohammad 
Published: May 2023

Abstract
Plants respond to heavy metals stress by the activation of stress responsive genes to establish biochemical defense mechanisms against the stress. We have investigated the molecular and biochemical response of Cannabis sativa to cadmium (Cd) stress in the presence of molybdenum (Mo) foliar treatments. Various parameters were inter-correlated i.e., Plant biomass, Cd phyto-accumulation, transcript level/expression of CBF/DREB genes, concentrations of free proline, polyphenolics and chlorophyll contents. Phenolic compounds were analyzed by High Performance Liquid Chromatography Diode Array Detector (HPLC-DAD) and Cd contents were analyzed by Atomic Absorption Spectrophotometer. Biomass and chlorophyll contents decreased while the concentrations of free proline and polyphenolics increased under Cd stress. Dry biomass of the all the plant tissues and free proline in roots and leaves were most significantly increased with foliar spray of 2.00 ppm Mo. Nineteen (19) different polyphenolic compounds were identified and quantified in leaves and most of them were highly increased in concentration under Cd stress. The highest concentration was noted in Caffeoyl tyramine (1111 µg/g) in 2.00 ppm Mo treated plants. Transcript level of CBF/DREB genes were less as compared to Mo treated plants, where a high increase in transcript level of these genes were found. Expression of CBF /DREB genes showed significant positive correlations with dry-biomass, Cd accumulation and concentrations of free proline and polyphenolics in leaves. The results suggested that Mo increased the expression of CBF/DREB genes and subsequently the concentration of phenolic compounds and free proline under Cd stress.
Development of the potential synthesized compound to cause triple negative breast cancer cell death through intrinsic apoptosis mechanism
Authors: Tahmasvand Raheleh , Dehghani Soudeh , Kooshafar Zahra , Almasirad Ali , Salimi Mona 
Published: May 2023

AbstractTriple-negative breast cancer (TNBC) is a subtype of breast cancer with poor clinical outcome, and currently no effective targeted therapies are available. Since cancer develops owing to deregulation of apoptosis, employing therapeutic strategies with ability to target the molecules involved in apoptosis induction, would provide a valid approach to hinder tumor progression. Hydrazide-hydrazones and oxamide molecules are the subject of intense studies due to their anticancer effects via apoptosis induction. In the present study, we attempted to elucidate the mechanism of action of a synthesized compound (compound A) and understand if the cell death occurs through inducing the apoptosis. These properties were investigated using the, annexin/PI and Western blotting analyses, DAPI staining, and mitochondrial membrane potential probe. Compound A also impeded the tumor growth in a 4T1 syngeneic mouse model as evidenced by hematoxylin and eosin staining of the tumors. Apart from that, it significantly diminished the expression of pro-caspase-3, Ki67 and CD31 markers in the tumor sections. Conclusively, this study for the first time reports the anti-cancer efficacy of compound A in both in vitro and in vivo models, which may serve as a potent candidate in triple negative breast cancer (TNBC) treatment.
Development and feasibility of an intervention toward a PErsonalized Pathway for ONcology patients (PEPON) in a French healthcare organization: a mixed method research protocol
Authors: SALMA Israa , PALMA Mario DI , PAPAGEORGIOU Loula , SCOTTE Florian , MINVIELLE Etienne 
Published: May 2023

Abstract
Introduction: In oncology, the care process of chronic patients needs to be personalized. This entails considering both clinical aspects and aspects directly related to personal expectations and socioeconomic status. However, designing personalized care interventions to meet each patient’s needs and demands poses an organizational problem. Therefore, the PErsonalised Pathway for ONcology patients (PEPON) -project aims to develop and evaluate an intervention to improve personalisation for oncology patient pathways.Method This protocol follows the Medical Research Council guidelines for complex intervention. It describes two phases of the PEPON project. The first phase is the design of the intervention; it involves a qualitative study in the one-day hospital (ODH) at Gustave Roussy (GR) Cancer Centre. Data collection (ongoing activity) relies on interviews with relevant professionals and patients, ODH observations, and documents. Data are coded and analysed using the “managing singularity on a large scale (GERS)” model for personalisation. This will identify areas for improvement on the current patient’s pathway, leading to the development of the intervention. The second phase is the piloting and feasibility evaluation. The intervention will be piloted and its feasibility tested on a cohort of patients passing through the ODH using a mixed method approach. Qualitative analysis will be based on interviews to investigate professionals’ experiences, observations, and documents. Quantitative analysis will be based on a comparison with a sample that did not use the intervention. Outcomes will include measures of quality of life, patient experience and patient records.Discussion Using the GERS concept, we would design a comprehensive organisational intervention to improve personalisation across the patient pathway, through the following steps: identification of patient needs and demands, design of a personalised care plan based on the existing organizations’ offers, and finally distribution of care plan. Through the pilot and feasibility phase, we will adapt the intervention to the local context before proceeding with a full-scale standardisation and implementationConclusion The results of the pilot study will inform the protocol of the randomised control trial to evaluate the intervention’s impact.
Bovine milk-derived exosomes attenuate NLRP3 inflammasome and NF-κB signaling in the lung during neonatal necrotizing enterocolitis
Authors: Filler Rachel , Yeganeh Mina , Li Bo , Lee Carol , Alganabi Mashriq , Hock Alison , Biouss George , Balsamo Felicia , Lee Dorothy , Miyake Hiromu , Pierro Agostino 
Published: May 2023

Abstract
Purpose Necrotizing enterocolitis (NEC), an inflammatory intestinal disease common in premature infants, has been associated with the development of lung damage. Toll-like receptor 4 has been shown to regulate inflammation in the NEC lungs, however, other important inflammatory mechanisms have not been thoroughly investigated. In addition, we reported that milk-derived exosomes were able to attenuate intestinal injury and inflammation in experimental NEC. This study aims to (i) investigate the role of the NLRP3 inflammasome and NF-κB pathway in regulating lung damage during experimental NEC; and (ii) evaluate the therapeutic potential of bovine milk exosomes in reducing lung inflammation and injury during NEC.Methods NEC was induced by gavage feeding of hyperosmolar formula, hypoxia, and lipopolysaccharide administration in neonatal mice from postnatal days 5–9. Exosomes were obtained by ultracentrifugation of bovine milk and administered during each formula feed.Results The lung of NEC pups showed increased inflammation, tissue damage, NLRP3 inflammasome expression, and NF-κB pathway activation, which were attenuated upon exosome administration.Conclusion Our findings suggest that the lung undergoes significant inflammation and injury following experimental NEC which are attenuated by bovine milk-derived exosomes. This emphasizes the therapeutic potential of exosomes not just on the intestine but also on the lung.
Target Validation and Structure-Based Virtual Screening to Discover Potential Lead Molecules against the oncogenic NSD1 histone methyltransferase
Authors: Bhat Zahid Rafiq , Gahlawat Anuj , Kumar Navneet , Sharma Nisha , Tikoo Kulbhushan , Garg Prabha 
Published: May 2023

Abstract

The aim of the study was to validate Nuclear receptor-binding SET Domain NSD1 as a cancer drug target followed by the design of lead molecules against NSD1. TCGA clinical data, molecular expression techniques were used to validate the target and structure-based virtual screening was performed to design hits against NSD1. Clinical data analysis suggests the role of NSD1 in metastasis, prognosis and influence on overall survival in various malignancies. Furthermore, the mRNA and protein expression profile of NSD1 was evaluated in various cell lines. NSD1 was exploited as a target protein forin silicodesign of inhibitors using two major databases including ZINC15 and ChemDiv by structure-based virtual screening approach. Virtual screening was performed using the pharmacophore hypothesis designed with a protein complex S-adenosyl-l-methionine (SAM) as an endogenous ligand. Subsequently, a combined score was used to distinguish the top 10 compounds from the docking screened compounds having high performance in all four scores (docking score, XP, Gscore, PhaseScreenScore, and MMGBSA delta G Bind). Finally, the top three Zinc compounds were subjected to molecular dynamic simulation. The binding MMGBSA data suggests that ZINC000257261703 and ZINC000012405780 can be taken forin vitroandin vivostudies as they have lesser MMGBSA energy towards the cofactor binding site of NSD1 than the sinefungin. Our data validates NSD1 as a cancer drug target and provides promising structures that can be utilized for further lead optimization and rational drug design to open new gateways in the field of cancer therapeutics.
Increased vegetation sensitivity to meteorological drought from spring to autumn across European biomes
Authors: vicente-serrano Sergio , Tian Feng , Cai Zhanzhang , Conradt Tobias , Boincean Boris , Murphy Conor , Farizo Begoña , Grainger Sam , Moreno Juan Ignacio López , Eklundh Lars , Jin Hongxiao 
Published: May 2023

Abstract
Europe has experienced severe drought events in recent decades, with the diverse distribution of vegetation, varied drought characteristics, and other concurrent hydroclimatic factors making it challenging to understand how vegetation responds to drought. To shed light on this issue, we use an ensemble of vegetation growth indicators to analyze the instantaneous vegetation response to meteorological drought across European biomes. We find increased vegetation sensitivity to drought along canopy development during a year, with mean sensitivities rising from ‒0.01 in spring to 0.28 in autumn and the percentage of drought-susceptible areas rising from 18.5% to 57.8% across European biomes. Temporally, the sensitivity of vegetation growth to meteorological drought is exacerbated by soil water shortage. Spatially, the vegetation-drought sensitivity is related to vapor pressure deficit (ensemble mean correlation coefficients 0.71–0.87) and partly to atmospheric CO2 concentration with dependencies on the maturity of the canopy (correlations ‒0.34 in spring and 0.81 in autumn), but only weakly to soil moisture (correlations 0.06–0.19). Our results highlight the spatiotemporal variations of vegetation-drought associations and sensitivities, as well as the roles of concurrent hydroclimatic factors. These patterns may have important implications for atmospheric carbon reduction, ecosystem stability, biodiversity conservation, and vegetation feedback on regional climate.
Towards a scalable approach to assess speech organization across the psychosis-spectrum -
Online assessment in conjunction with automated transcription and extraction of speech measures.
Authors: Cummins Nicholas , Arribas Maite , Gibbs-Dean Toni , Molina Elena , Sethi Divina , Kempton Matthew , Morgan Sarah , Spencer Tom , Diederen Kelly , Olah Julianna 
Published: May 2023

Abstract
Automatically extracted measures of speech constitute a promising marker of psychosis as disorganized speech is associated with psychotic symptoms and predictive of psychosis-onset. The potential of speech markers is, however, hampered by (i) lengthy assessments in laboratory settings and (ii) manual transcriptions. We investigated whether a short, scalable data collection (online) and processing (automated transcription) procedure would provide data of sufficient quality to extract previously validated speech measures. To evaluate the fit of our approach for purpose, we assessed speech in relation to psychotic-like experiences in the general population. Participants completed an 8-minutes long speech task online. Sample 1 included measures of psychometric schizotypy and delusional ideation (N = 446). Sample 2 included a low and high psychometric schizotypy group (N = 144). Recordings were transcribed both automatically and manually, and connectivity, semantic and syntactic speech measures were extracted for both type of transcripts. 73%/86% participants in sample 1/2 completed the experiment. Nineteen out of 25 speech measures were strongly (r > 0.7) and significantly correlated between automated and manual transcripts in both samples. Amongst the 14 connectivity measures, 11 showed a significant relationship with delusional ideation. For the semantic and syntactic measures, On Topic score and the Frequency of personal pronouns were negatively correlated with both schizotypy and delusional ideation. Combined with demographic information, the speech markers could explain 11–14% of the variation of delusional ideation and schizotypy in Sample 1 and could discriminate between high-low schizotypy with high accuracy (0.72 − 0.70, AUC = 0.78–0.79) in Sample 2. The moderate to high retention rate, strong correlation of speech measures across manual and automated transcripts and sensitivity to psychotic-like experiences provides initial evidence that online collected speech in combination with automatic transcription is a feasible approach to increase accessibility and scalability of speech-based assessment of psychosis.
Improving Lithium-ion Cells by Replacing Polyethylene Terephthalate Jellyroll Tape
Authors: Adamson Anu , Tuul Kenneth , Botticher Tom , Azam Saad , Garayt Matthew , Metzger Michael 
Published: May 2023

Abstract
Polyethylene terephthalate (PET) tape is widely used by well-known lithium-ion battery manufacturers to prevent electrode stacks from unwinding during assembly. PET tape is selected since it has suitable mechanical and electrical properties, but its chemical stability has been largely overlooked. In the absence of effective electrolyte additives, PET can depolymerize into its monomer dimethyl terephthalate (DMT), which is an unwanted redox shuttle that induces significant self-discharge in a lithium-ion cell. This study presents a chemical screening experiment to probe the PET decomposition mechanism involving in situ generated methanol and lithium methoxide from dimethyl carbonate, one of the most common electrolyte solvents in lithium-ion cells. By screening other polymers, it is found that polypropylene (PP) and polyimide (Kapton) are stable in the electrolyte. Finally, it is demonstrated that reversible self-discharge of LiFePO4/graphite cells can be virtually eliminated by replacing PET jellyroll tape with chemically stable PP tape.
WITHDRAWN: Bidirectional symmetric 1.6 Tbps hybrid TWDM-OFDM PON using 2D spectral/spatial SWZCC code
Authors: SinghSH  
Published: May 2023

Abstract

The hybrid time and wavelength division multiplexing passive optical network (TWDM-PON) with a 1:128 split ratio and 16- quadrature amplitude modulation orthogonal frequency division multiplexing (QAM- OFDM) modulation system using optical code division multiple access (OCDMA) code results in high transmission security, channel capacity and robustness against interference. Thus in this paper, a bidirectional symmetric 1.6 Tbps hybrid TWDM-OFDM PON system using two-dimensional spectral/spatial-single weight zero cross-correlation (2D-S/S SWZCC) code supporting a large number of users for long-reach as well as high- speed applications has been proposed and analysed considering fiber impairments and noise. Numerically calculated receiver sensitivity (RS) and splitter power budget (SPB) are -36dBm and 23dB for downstream as well as RS and SPB for upstream transmission of -33dBm and 10dB respectively showing the supremacy of using 2D-S/S SWZCC code in the system than other OCDMA codes. Also, the simulation results show that the proposed system using 2D-SS SWZCC code provides a faithful 120km transmission distance as well as a high transmission rate of 16×15/20Gbps with a maximum received power of -32.8dBm supporting more than 190 simultaneous users. The designed PON system offers the best performance over other conventional PONs. The number of users can be increased up to 250 with an optical signal-to-noise ratio of 110 dB and -30 dBm received power.
Pan-cancer transcriptomic analysis of CNS tumor stroma identifies a population of perivascular fibroblasts that predict poor immunotherapy response in glioblastoma patients
Authors: Zarodniuk Maksym , Steele Alexander , Lu Xin , Li Jun , Datta Meenal 
Published: May 2023

Abstract
Excessive deposition of extracellular matrix (ECM) is a hallmark of solid tumors; however, it remains poorly understood which cellular and molecular components contribute to the formation of ECM stroma in central nervous system (CNS) tumors. Here, we undertook a pan-CNS analysis of retrospective gene expression datasets to characterize inter- and intra-tumoral heterogeneity of ECM remodeling signatures in both adult and pediatric CNS disease. We found that CNS lesions – glioblastoma in particular – can be divided into two ECM-based subtypes (ECMhi and ECMlo) that are influenced by the presence of perivascular cells resembling cancer-associated fibroblasts (CAFs). We show that perivascular fibroblasts activate chemoattractant signaling pathways to recruit tumor-associated macrophages, and promote an immune-evasive, stem-like cancer cell phenotype. Our analysis reveals that perivascular fibroblasts are correlated with unfavorable response to immune checkpoint blockade in glioblastoma and poor patient survival across a subset of CNS tumors. We provide insights into novel stroma-driven mechanisms underlying immune evasion and immunotherapy resistance in CNS tumors like glioblastoma, and discuss how targeting these perivascular fibroblasts may prove an effective approach to improving treatment response and patient survival in a variety of CNS tumors.