bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demographic, pain, and quality of life factors in unilateral and bilateral total knee arthroplasty: a population-based retrospective cohort study of 23,033 patients
Authors: Leech Samantha A , Khaira Akashroop , Epp Spencer , Schneider Geoff , Werle Jason , Ng Richard , Harris Ashley D , Manske Sarah L 
Published: July 2023

Abstract

Background
The Alberta Bone and Joint Health Institute (ABJHI) has a comprehensive registry capturing clinical, demographic, and patient-reported outcome measures on all knee arthroplasties performed in the province of Alberta, Canada. This large database provides an opportunity to study the outcomes of total knee arthroplasties (TKA) in patients who underwent unilateral or bilateral procedures.
Methods
Pain and quality of life outcomes of 29,157 TKAs performed in 23,033 patients for knee osteoarthritis performed in Alberta, Canada from January 2013 to February 2020 were examined. This study: (1) investigates whether having both knees replaced results in better pain and quality of life outcomes than replacing only one knee (using multivariate analysis of variance) and (2) identifies if pain and quality of life outcomes impact the likelihood of needing surgery in the contralateral knee after a first TKA (using univariate and multivariate Cox proportional hazards models). Patient-reported outcomes were measured using questionnaires: the Western Ontario and McMaster Universities Arthritis Index (WOMAC) to assess knee pain, and the EuroQol-5D-5L (EQ-5D) to assess quality of life.
Results
Patients who had surgery on both knees had approximately 5% better WOMAC (pain) and EQ-5D (quality of life) questionnaire scores 12 months post-surgery than those who had surgery on only one knee. Additionally, for every 1-point higher pre-operative WOMAC pain score (i.e., less pain) there was a 1% decreased likelihood of undergoing a contralateral TKA compared to a 46% decreased likelihood per 1-point improvement in quality of life. Dementia, back pain, and hospital readmission were associated with a 68%, 17%, and 44% decreased likelihood of contralateral TKA respectively. By contrast, patients with obesity were 36% more likely to undergo bilateral TKA (p < 0.05).
Conclusions
Preoperative knee pain and quality of life play a statistically significant role in determining which patients undergo bilateral TKA. Additionally, bilateral TKA resulted in better patient-reported outcomes compared to unilateral TKA. Though clinical significance cannot be drawn from the low response rates for the patient-reported outcomes, these findings provide a basis for future research on patient-reported outcomes within the Canadian healthcare system.
Positioning, power and agency in postgraduate primary care supervision: a study of trainee narratives
Authors: Jackson Dawn , Josephine Brady , Donna Dawkins 
Published: July 2023

Abstract
Background

Postgraduate supervision takes place within complex training environments, where experiences are shaped by the socio-cultural context and wider profession, and where tensions permeate. Bordin’s working alliance-based model of supervision suggests that quality relationships encompass agreement on the goals and tasks of supervision, in the context of an emotional bond. However, as trainees and their supervisors navigate the demands of providing safe clinical care, alongside educational support, disagreement on expectations for supervision may emerge. By applying a critical lens, this research draws on positioning theory to explore General Practice trainees’ experiences of supervision.

Methods

In 2017-2019 a series of narrative interviews were undertaken with 13 General Practice trainees in the United Kingdom (UK). Participants were purposively sampled based on end-of-year performance, gender, training location and training status. Interviews were analysed using Brown and Gilligan’s Listening Guide, which was adapted to incorporate an exploration of positioning, power and agency.

Results

Trainees appeared to hold variable positions, such as ‘insiders’, ‘outsiders’, ‘peers’ and ‘problem trainees’. Supervisors, through talk and the degree of access afforded, contributed to this positioning. Some trainees viewed their supervisors as brokers and guides as they navigated their training, whilst others were suspicious of the supervisor role. For trainees who raised concerns about their supervisor through formal channels, results were not often satisfactory. Others chose to navigate difficulty in supervision through informal means. This typically involved mastery of artefacts of training, such as the electronic appointment book or training portfolio.

Conclusions

This paper builds on Bordin’s model of supervision to encourage greater clarity in supervisory discussions, exploring assumptions, and recognising the influences of environment, power, positioning, and agency. We have developed a Model of the Supervisory Alliance in Postgraduate GP Training (MSA-GP) to serve as a springboard for discussion for trainees and their supervisors.
Deciphering intratumoral heterogeneity of hepatocellular carcinoma with microvascular invasion with radiogenomic analysis
Authors: Wang Yi , Zhu Gui-Qi , Yang Rui , Wang Cheng , Qu Wei-Feng , Chu Tian-Hao , Yang Li , Zhou Chang-Wu , Miao Geng-Yun , Tang Zheng , Yang Chun , Liu Wei-Ren , Shi Ying-Hong , Zeng Meng-Su 
Published: July 2023

Abstract
Background and aims:
The recurrence and metastasis of hepatocellular carcinoma (HCC) are mainly caused by microvascular invasion (MVI). Our study aimed to uncover the cellular atlas of MVI+ HCC and investigate the underlying immune infiltration patterns with radiomics features.
Methods
Three MVI positive HCC and three MVI negative HCC samples were collected for single-cell RNA-seq analysis. 26 MVI positive HCC and 30 MVI negative HCC tissues were underwent bulk RNA-seq analysis. For radiomics analysis, radiomics features score (Radscore) were built using preoperative contrast MRI for MVI prediction and overall survival prediction. We deciphered the metabolism profiles of MVI+ HCC using scMetabolism and scFEA. The correlation of Radscore with the level of APOE+ macrophages and iCAFs was identified. Whole Exome Sequencing (WES) was applied to distinguish intrahepatic metastasis (IM) and multicentric occurrence (MO). Transcriptome profiles were compared between IM and MO.
Results
Elevated levels of APOE + macrophages and iCAFs were detected in MVI+ HCC. There was a strong correlation between the infiltration of APOE+ macrophages and iCAFs, as confirmed by immunofluorescent staining. MVI positive tumors exhibited increased lipid metabolism, which was attributed to the increased presence of APOE + macrophages. APOE+ macrophages and iCAFs were also found in high levels in IM, as opposed to MO. The difference of infiltration level and Radscore between two nodules in IM was relatively small. Furthermore, we developed Radscore for predicting MVI and HCC prognostication that were also able to predict the level of infiltration of APOE+ macrophages and iCAFs.
Conclusion
This study demonstrated the interactions of cell subpopulations and distinct metabolism profiles in MVI+ HCC. Besides, MVI prediction Radscore and MVI prognostic Radscore were highly correlated with the infiltration of APOE+ macrophages and iCAFs, which helped to understand the biological significance of radiomics and optimize treatment strategy for MVI+ HCC.
An In-silico Analysis of OGT gene association with diabetes mellitus
Authors: Ayodele Abigail O. , Udosen Brenda , Oluwagbemi Olugbenga O. , Oladipo Elijah K. , Omotuyi Idowu , Isewon Itunuoluwa , Nash Oyekanmi , Soremekun Opeyemi , Fatumo Segun 
Published: July 2023

Abstract
O-GlcNAcylation is a nutrient-sensing post-translational modification process. This cycling process involves two main proteins; the O-linked N-acetylglucosamine transferase (OGT) catalyzing the addition, and the glycoside hydrolase OGA (O-GlcNAcase) catalyzing the removal of the O-GlCNAc moiety on nucleocytoplasmic proteins. This process is necessary for various important cellular functions. The O-linked N-acetylglucosamine transferase (OGT) gene is responsible for the production of the OGT protein. Several studies have shown the overexpression of this protein to have biological implications in metabolic diseases like cancer and diabetes mellitus (DM). In this study, we retrieved a total of 159 SNPs with clinical significance from the SNPs database and we probed the functional effects, stability profile, and evolutionary conservation of these to determine their fit for this research. We then identified 7 SNPs (G103R, N196K, Y228H, R250C, G341V, L367F, and C845S) with predicted deleterious effects across the four tools used (PhD-SNPs, SNPs&Go, PROVEAN, and PolyPhen2). Proceeding with this, we used ROBETTA, a homology modeling tool, to model the proteins with these point mutations and carried out a structural bioinformatics method – molecular docking – using the Glide model of the Schrodinger Maestro suite. We used a previously reported inhibitor of OGT, OSMI-1, as the ligand for these mutated protein models, and as a result, very good binding affinities and interactions were observed between this ligand and the active site residues within 4Å of OGT. We conclude that these mutation points may be used for further downstream analysis as drug targets for the treatment of diabetes mellitus.
GRP94 is an IGF-1R Chaperone and Regulates Beta Cell Death in Diabetes
Authors: Wang Hongjun 
Published: July 2023

Abstract

High workload-induced cellular stress can cause pancreatic islet β cell death and dysfunction, or β cell failure, a hallmark of type 2 diabetes mellitus. Thus, activation of molecular chaperones and other stress-response genes prevents β cell failure. To this end, we have shown that deletion of the glucose-regulated protein 94 (GRP94) in Pdx1+ pancreatic progenitor cells led to pancreas hypoplasia and reduced β cell mass during pancreas development in mice. Here we show that GRP94 was involved in β cell adaption and compensation (or failure) in islets from leptin receptor-deficient (db/db) mice in an age-dependent manner. GRP94-deficient cells were more susceptible to cell death induced by various diabetogenic stress conditions. We also identified a new client of GRP94, insulin-like growth factor-1 receptor (IGF-1R), a critical factor for β cell survival and function that may mediate the effect of GRP94 in the pathogenesis of diabetes. This study has identified essential functions of GRP94 in β cell failure related to diabetes.
The complete chloroplast genome and phylogenetic analysis of Dahlia pinnata Cavanilles 1791(Asteraceae: Dahlia)
Authors: Duan Shan-De , Zheng Kua-Yue , Liu Yang , Li Xing-Ru , Xiang Di-Ying , Hao Li-Hong , Chen Duan-Fen , Niu Shan-Ce 
Published: July 2023

Abstract
Dahlia pinnata Cavanilles 1791 is an important ornamental plant worldwide. The chloroplast genome has obvious advantages in studies of systematic evolution at the plant classification and species level, making it an important resource for phylogenetic research.Here, we sequenced the full chloroplast genome from D. pinnata 'Chocolate' and found that it exhibited a typical tetrad structure. The full-length D. pinnata chloroplast genome was 152,107 bp, with a GC content of 38.45%. The genome included an 83,704 bp large single-copy (LSC) region, an 18,347 bp small single-copy (SSC) region, and a pair of 25,028 bp inverted repeats (IRa and IRb). A total of 134 genes were annotated, including 86 protein-coding genes, 38 transfer RNA genes, 8 ribosomal RNA genes, and 2 pseudogenes. Analysis of password preferences shows that passwords chosen by D. pinnata tend to end with A/U. A total of 161 SSR markers were detected in the simple sequence repeat (SSR) analysis. Phylogenetic analysis that the Dahlia species formed a monophyly. Dahlia was clustered with Cosmos-Bidens, which differed from studies using nuclear genomic DNA. We suggest that nuclear-cytoplasmic incongruences may be widespread in Asteraceae, and should be thoroughly evaluated in order to understand the true evolutionary history of this economically-important group of plants.
Carbamylated erythropoietin alleviates seawater immersion-aggravated acute lung injury via inhibiting ferroptosis after Traumatic Brain Injury.
Authors: zhang hao , lin long , ye dan , xu yongjun , wang shousen 
Published: July 2023

Abstract
Acute lung injury (ALI) is a life-threatening disorder associated with high morbidity and mortality rates. It is characterized by reactive oxygen species (ROS) generation and epithelial apoptosis. Ferroptosis, another form of cell death triggered by the accumulation of bioactive iron and ROS generation, has been implicated in the pathogenesis of ALI. This study aimed to explore the role of Carbamylated erythropoietin (C-EPO) in treating seawater drowning (SWD)-induced acute lung injury (SWD-ALI) and SWD-ALI aggravated by traumatic brain injury (SWD + TBI). The study established rat models of SWD-ALI and SWD + TBI-induced ALI to investigate the effects of C-EPO on ferroptosis and autophagy in these conditions. Rat models of SWD-ALI and SWD + TBI-induced ALI were created to evaluate the impact of C-EPO. Lung histopathology, tissue damage, oxidative stress, and lung injury severity were assessed to determine the effectiveness of C-EPO treatment. The study also examined the influence of C-EPO on ferroptosis and autophagy. Key proteins in the mTOR signaling pathway, including p-mTOR, P62, Beclin1, and the LC3II/LC3I ratio, were analyzed to elucidate the underlying mechanisms. C-EPO treatment significantly improved lung histopathology, reduced tissue damage, mitigated oxidative stress, and attenuated lung injury severity in the SWD-ALI and SWD + TBI-induced ALI rat models. C-EPO demonstrated protective effects against septicemia-induced ferroptosis in the lung tissue of rats with SWD + TBI-induced ALI. Furthermore, C-EPO treatment inhibited autophagy activation in SWD + TBI-induced ALI by modulating the mTOR signaling pathway, as evidenced by decreased expression of p-mTOR, P62, Beclin1, and a modified LC3II/LC3I ratio.The findings of this study suggest that C-EPO shows promise as a therapeutic agent for managing SWD-ALI and SWD + TBI-induced ALI. By targeting ferroptosis and suppressing autophagy via modulation of the mTOR signaling pathway, C-EPO provides protection against lung injury. These results contribute to a deeper understanding of the underlying mechanisms of ALI and offer valuable insights into potential therapeutic interventions for this life-threatening condition.
Integrative analysis reveals the role of THBS1 in IgG4-related disease
Authors: Yan Songxin , Yu Tian , Peng Yu , Li Zhan , Wu Ziyan , Su Pengfei , Zhang Yingjing , Xu Honglin , Feng Futai , Huang Yuan , Li Yongzhe 
Published: July 2023

Abstract
Background: IgG4-related disease (IgG4-RD) is a heterogeneous immune-mediated fibroinflammatory disorder. By utilizing the Gene Expression Omnibus (GEO) database and single-cell sequencing data, we aimed to construct a comprehensive transcriptomic profile of IgG4-RD and identify hub genes involved in its pathogenesis.
Results: IgG4-RD-related differentially expressed genes were found to be coenriched in immune- and platelet-related biological functions or signaling pathways. WGCNA analysis showed that five hub genes (GNG11, PDE5A, PLK2, PROS1, and thrombospondin 1 [THBS1]) were upregulated in LSG and PBMCs. Further analysis of the protein–protein interaction network revealed that THBS1 was the key gene. Plasma THBS1 levels were significantly elevated in patients with IgG4-RD compared with those in healthy controls (p< 0.0001). Immune infiltration analysis revealed a correlation between THBS1expression and various immune infiltrating cells. Single-cell sequencing analysis indicated that THBS1 was predominantly expressed in classical monocytes of PBMCs derived from patients with IgG4-RD.
Conclusion: This study revealed the potential pathogenic mechanisms of THBS1 in IgG4-related disease and identified THBS1 as a potential diagnostic biomarker for the disease.
A retrospective study of paraganglioma of the urinary bladder and literature review
Authors: Zhang Zhijun , Zhao Yi , Wang ShiJun , Wen Jin , Wang Dong , Ji ZhiGang , Zhang YuShi , Li HanZhong 
Published: July 2023

Abstract
Objective
To retrospect and summarize the characteristics and therapy of paraganglioma of the urinary bladder (PUB).
Method
Patients who underwent the operation in Peking Union Medical College Hospital from January 2012 to December 2021 were reviewed for this retrospective study.
Results
A total of 29 patients, comprising 9 males (31%) and 20 females (69%) were included. The main manifestations were hypertension, palpitation, and micturition syncope. 8 patients had an increased 24-hour urinary catecholamine, and 7 of them were increased norepinephrine. 7 patients’ normetanephrine were increased. 6/18 metaiodobenzylguanidine and 8/22 octreotide imaging were positive. 15 cases took laparoscopic partial cystectomy and 14 took transurethral resection of bladder tumor. Of all the patients, the immunohistochemical index of Melan-A, AE1/AE3 and α-inhibin were negative, while (chromogranin A) CgA, S-100 and (succinate dehydrogenase) SDH B were positive. Ki-67 of 28/29 cases are under 5%, and 1 case whose Ki-67 was 20% was diagnosed with malignant PUB. 25 patients had a regular follow-up, 1 patient died in 1 year after the surgery, 2 patients were lost found, and 2 patients had a recurrence. The symptoms all disappeared or relieved after the surgery.
Conclusion
Transurethral surgery approach fits for PUB tumors whose sizes are under 3cm or that protrudes into the bladder and can significantly reduce the postoperative hospital stay. Early detection and treatment are effective, and regular review is necessary after the surgery.
Mapping quantitative trait loci regions associated with Marek’s Disease on chicken autosomes by means of selective DNA pooling
Authors: Lipkin Ehud , Smith Jacqueline , Soller Morris , Burt David W. , Fulton Janet E. 
Published: July 2023

Abstract
Marek’s Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P- and LD- values were used to asses the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD.
Mapping the Pandemic: A Review of GIS-based Spatial Modeling of COVID-19
Authors: Aboalyem Mustafa Shebani , Ismail Mohd Tahir 
Published: July 2023

Abstract
According to the World Health Organization (WHO), COVID-19 has caused more than 6 million deaths, while over 600 million people are infected. With regard to the tools and techniques of disease analysis, spatial analysis is increasingly being used to analyze the impact of COVID-19. The present review offers an assessment of researches that used regional data systems to study COVID-19 epidemic that was published between 2020 and 2022. The research work focuses at: categories the area, authors, methods, and procedures used by the authors and the results of their findings. This input will enable the contrast of different spatial models used for regional data systems with COVID-19. Our outcomes showed an increase in the use of geographically weighted regression and Moran I spatial statistical tools applied to better spatial and time-based gauges. We have also found an increase in the use of local models compared to other spatial statistics models/methods.
Malaria Mimicking Acute Abdomen: An Extremely Rare Case Report
Authors: Hassan YonisYusuf , Ali Abdullahi Sheikh , Mohamed Ali Jimale 
Published: July 2023

Abstract

Malaria is concentrated mostly in poor low-income tropical and subtropical countries of the Africa due to Anopheles gambiae of predominantly parasite species Plasmodium falciparum. In Somalia, Malaria is considered an endemic-area across the country and progress to cause multi-organ failure both in adults and children. This case report aims to shed light on a rare suspected case of malaria mimicking acute abdominal pain which rarely occur compared to other clinical findings of Malaria. Here, we present a 9-year-old male patient from south Somalia with a complaint of severe abdominal pain, nausea, and vomiting presented to the emergency department, those clinical findings not suggesting being Malaria symptoms. Clinical findings of Malaria may include nonspecific findings such as headache, fatigue, myalgia, and arthralgia associated with fever attacks. In addition to affecting organs like the brain, lungs, and kidneys, severe malaria frequently requires intensive care medicine. It can also be worsened by systemic infections and widespread bleeding. In conclusion, after a detailed history, careful physical examination, and comprehensive evaluation, the patient was diagnosed with acute abdominal pain caused by Malaria. Therefore, it is successfully treated by Artemisinin-based combination therapy (ACT) to eliminate the parasite load rapidly, as a first-line malaria therapy in endemic countries, and currently standard care for the treatment of uncomplicated malaria.
Utilizing propensity score matching methodology to assess the impact of adjuvant radiotherapy on the prognosis of men diagnosed with breast cancer
Authors: Meng Jing , Wu Qi , Wang Jianlin , Zhao Aimei , Ren Huiwen , Sun Zhiqiang , Luo Judong 
Published: July 2023

Abstract
Background
Male breast cancer (MBC) is a rare condition, and the effectiveness of radiotherapy in treating MBC patients remains uncertain. This study aimed to investigate the role of adjuvant radiotherapy (RT) in the management of MBC.
Methods
MBC patients from the Surveillance Epidemiology and End Results (SEER) database were included in the study and were divided into RT and no-RT groups. A 1:1 propensity score matching (PSM) method was employed to balance baseline characteristics. Kaplan-Meier curves were used to evaluate the impact of RT on overall survival (OS) and breast cancer-specific survival (BCSS). Cox analyses were conducted to identify factors associated with survival. Subgroup analysis was performed to identify subgroups of MBC patients who might benefit from RT.
Results
In the matched cohort, the 5-year OS and BCSS rates were higher in the RT group compared to the no-RT group (p = 0.023, p = 0.035). Univariate and multivariate analysis demonstrated significant differences in both OS and BCSS associated with RT (p = 0.024, p = 0.037, p = 0.025, p = 0.028). Forest plots revealed a greater OS benefit in patients with T1 stage, age ≥ 60 years, estrogen receptor positivity (ER+), absence of distant metastasis (M0), married status, and with local or regional metastases. Furthermore, a greater BCSS benefit was observed in patients aged ≥ 60 years, ER+, progesterone receptor negativity (PR-), M0, married status, and with regional metastases who received RT for MBC.
Conclusion
RT in MBC patients is associated with improved survival and is recommended for patients aged ≥ 60 years with ER+, PR-, M0, married status, and regional metastases.