bluuebunny/crossref_metadata_2025_split huggingface dataset:
Processing negative autobiographical memories in a foreign language
Authors: Ortigosa-Beltrán Isabel , Jaén Irene , García-Palacios Azucena 
Published: December 2022

AbstractThe use of a foreign language has been introduced in the clinical setting as a form of emotional distance to help deal with negative experiences. However, its evidence of reducing emotionality during the emotional processing is still scarce. This study aims to test whether the description and processing of a traumatic or highly emotional event in a foreign language could modulate the strength of the connection between traumatic symptomatology and emotional reaction. For this purpose, a sample of 128 healthy participants completed a series of questionnaires via an online platform. Firstly, their levels of distress, arousal and valence were assessed in their native language. Secondly, they were assigned to either the native language or the foreign language group and described a negative childhood event in the assigned language, followed by five questions adapted from Foa and Rothbaum (1998) and related to the event. Next, their emotionality was assessed again in their native language. Finally, a questionnaire of traumatic stress symptoms and an avoidance scale were completed. Multivariate regression analyses showed that the relationship between traumatic symptomatology and emotionality was moderated by the language of processing the negative event. Traumatic symptomatology was more strongly associated with distress and arousal change when the task was performed in the native language. These findings suggest the influence of a foreign language on emotional reactivity when a negative experience is processed, which could be an essential tool in the treatment of disorders related to stress and trauma.
Curcumin combined with verapamil improve cardiovascular phenotype of a Williams-Beuren Syndrome mice model reducing oxidative stress
Authors: Abdalla Noura , Ortiz-Romero Paula , Rodriguez-Rovira Isaac , Pérez-Jurado Luis A. , Egea Gustavo , Campuzano Victoria 
Published: December 2022

AbstractWilliams-Beuren syndrome (WBS) is a rare neurodevelopmental disorder with hallmarks in the cardiovascular manifestations and no well-defined therapeutic strategies. We investigated the progression of cardiovascular phenotype present in CD (complete deletion) mice, a murine model of WBS, after chronic treatment with curcumin and verapamil, both compounds with positive effects on related pathologies. Treatment was administered orally dissolved in drinking water. After measuring “in vivo” systolic blood pressure, aortic and left ventricular myocardium were histological and molecular analysed to determine the effects of treatment and its underlying mechanism in CD mice. We observed upregulated xanthine oxidoreductase (XOR) expression in both aortic and left ventricular myocardium of CD mice. This overexpression is concomitant with increased levels of nitrated proteins as example of byproduct-mediated oxidative stress damage, indicating of XOR-generated oxidative stress impact on the pathophysiology of cardiovascular manifestations in WBS, and suggesting that the inhibition of XOR and/or oxidative stress damage could help to ameliorate the severe cardiovascular injuries presented in WBS.
Indole produced during dysbiosis mediates host–microorganism chemical communication
Authors: Yang Rui-Qiu , Chen Yong-Hong , Wu Qin-Yi , Tang Jie , Niu Shan-Zhuang , Zhao Qiu , Ma Yi-Cheng , Zou Cheng-Gang 
Published: December 2022

AbstractAn imbalance of the gut microbiota, termed dysbiosis, has a substantial impact on host physiology. However, the mechanism by which host deals with gut dysbiosis to maintain fitness remains largely unknown. InC. elegans,E. coli, which is its bacterial diets, proliferates in its intestinal lumen during aging. Here, we demonstrate that progressive intestinal proliferation ofE. coliactivates the transcription factor DAF-16, which is required for maintenance of longevity and organismal fitness in worms with age. DAF-16 up-regulates two lysozymeslys-7andlys-8, thus limiting the bacterial accumulation in the gut of worms during aging. During dysbiosis, the levels of indole produced byE. coliare increased in worms. Indole is involved in the activation of DAF-16 by TRPA-1 in neurons of worms. Our finding demonstrates that indole functions as a microbial signal of gut dysbiosis to promote fitness of the host.
Follicular fluid metabolomic analysis in women with Hashimoto’s thyroiditis
Authors: da Silva Bastos Diana Caroline , Chiamolera Maria Izabel , Costa da Silva Renata Elen , Borges De Souza Maria Do Carmo , De Azevedo Antunes Roberto , Marinho De Souza Marcelo , Allemand Mancebo Ana Cristina , Fernandes Arêas Patrícia Cristina , Reis Fernando M. , Lo Turco Edson Guimarães , Bloise Flavia Fonseca , Ortiga-Carvalho Tania Maria 
Published: December 2022

AbstractHashimoto’s thyroiditis is an autoimmune thyroid disease characterized by hypothyroidism and a high level of anti-thyroid autoantibodies. This disease has been linked to a negative impact on female fertility, but the mechanisms are unclear. Ovarian follicular fluid appears to be the key to understanding how Hashimoto’s thyroiditis can affect fertility. Therefore, we aimed to evaluate the follicular fluid metabolic profile and its relationship with anti-thyroid autoantibody levels. For this, we collected follicular fluid from a total of 61 patients undergoing in vitro fertilization treatment, comprising 34 women with thyroid autoantibody positivity and 18 negative controls. Follicular fluid samples were analysed using metabolomics and thyroid autoantibodies were measured. Follicular fluid samples from Hashimoto’s thyroiditis patients presented 15 metabolites with higher concentrations than those in controls, which indicates five possible affected pathways: the glycerophospholipid, arachidonic acid, linoleic acid, alpha-linolenic acid, and sphingolipid metabolism pathways. These pathways are known to regulate ovarian functions. In addition, anti-thyroglobulin antibody concentrations were more than tenfold higher in women with Hashimoto’s thyroiditis than in controls, in both serum and follicular fluid. Our data showed that Hashimoto’s thyroiditis can change the metabolic profile of follicular fluid, suggesting a potential mechanistic explanation for the association of this disease with female infertility.
Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis
Authors: Merges Gina E. , Arévalo Lena , Lohanadan Keerthika , de Rooij Dirk G. , Jokwitz Melanie , Witke Walter , Schorle Hubert 
Published: December 2022

AbstractActin-related proteins (Arp) are classified according to their similarity to actin and are involved in diverse cellular processes.ACTL7Bis a testis-specific Arp and highly conserved in rodents and primates. ACTL7B is specifically expressed in round and elongating spermatids during spermiogenesis. Here, we have generated anActl7b-null allele in mice to unravel the role of ACTL7B in sperm formation. Male mice homozygous for theActl7b-null allele (Actl7b-/-) were infertile, while heterozygous males (Actl7b+/-) were fertile. Severe spermatid defects such as detached acrosomes, disrupted membranes and failed elongation of the axoneme start to appear at spermiogenesis step 9 inActl7b-/-mice, finally resulting in spermatogenic arrest. Abnormal spermatids, were degraded. Co-immunoprecipitation experiments identified interaction between ACTL7B and the LC8 dynein light chains DYNLL1 and DYNLL2, which are first detected in step 9 spermatids and mislocalized when ACTL7B is absent. Our data unequivocally establishes that mutations in ACTL7B are directly related to male infertility, pressing for additional research in men.Summary statementIn this study, Actl7b-deficient mice were generated. Loss of Actl7b leads to spermatogenic arrest in mice. ACTL7B interacts in with DYNLL1/DYNLL2 and seems to function in spermatid cytoskeleton.
Engineering of the EndogenousHBDpromoter increases HbA2
Authors: Boontanrart Mandy Y. , Mächler Elia , Ponta Simone , Nelis Jan C. , Preiano Viviana G. , Corn Jacob E. 
Published: December 2022

AbstractThe β-hemoglobinopathies, such as sickle cell disease and β-thalassemia, are one of the most common genetic diseases worldwide and are caused by mutations affecting the structure or production of β-globin subunits in adult hemoglobin. Many gene editing efforts to treat the β-hemoglobinopathies attempt to correct β-globin mutations or increase γ-globin for fetal hemoglobin production. δ-globin, the subunit of adult hemoglobin A2, has high homology to β-globin and is already pan-cellularly expressed at low levels in adult red blood cells. However, upregulation of δ-globin is a relatively unexplored avenue to increase the amount of functional hemoglobin. Here, we use CRISPR-Cas9 to repair non-functional transcriptional elements in the endogenous promoter region of δ-globin to increase overall expression of adult hemoglobin 2 (HbA2). We find that insertion of a KLF1 site alone is insufficient to upregulate δ-globin. Instead, multiple transcription factor elements are necessary for robust upregulation of δ-globin from the endogenous locus. Promoter edited HUDEP-2 immortalized erythroid progenitor cells exhibit striking increases ofHBDtranscript, from less than 5% to over 20% of total β-like globins. Edited CD34+ hematopoietic stem and progenitors (HSPCs) differentiated to primary human erythroblasts express up to 35%HBD. These findings add mechanistic insight to globin gene regulation and offer a new therapeutic avenue to treat β-hemoglobinopathies.
Exploiting effector - host interactions using TurboID-based proximity labeling
Authors: Shi Wei , Stolze Sara C , Nakagami Hirofumi , Misas Villamil Johana C. , Saur Isabel ML , Doehlemann Gunther 
Published: December 2022

AbstractPlant pathogens secrete effectors, which target host proteins to facilitate infection. TheUstilago maydiseffector UmSee1 is required for tumor formation in the leaf during infection of maize. UmSee1 interacts with maize SGT1 and blocks its phosphorylationin-vivo. In the absence of UmSee1,U. maydiscannot trigger tumor formation in the bundle sheath. However, it remains unclear which host processes are manipulated by UmSee1 and the UmSee1-SGT1 interaction to cause the observed phenotype. Proximity-dependent protein labeling involving the turbo biotin ligase tag (TurboID) for proximal labeling of proteins is a powerful tool for identifying the protein interactome. We have generated transgenicU. maydisthat secretes biotin ligase-fused See1 effector (UmSee1-TurboID-3HA) directly into maize cells. This approach, in combination with conventional co-immunoprecipitation allowed to identify additional UmSee1 interactors in maize cells. Collectively, our data identified three ubiquitin-proteasome pathway-related proteins (ZmSIP1, ZmSIP2, ZmSIP3) that either interact with or are close to UmSee1 during host infection of maize withU. maydis.ZmSIP3 represents a cell cycle regulator which degradation appears to be promoted in the presence of UmSee1. Our data provide a possible explanation for the requirement of UmSee1 in tumor formation duringU. maydis-Zea maysinteraction.
High genetic load without purging in a diverse species-at-risk
Authors: Taylor Rebecca S. , Manseau Micheline , Keobouasone Sonesinh , Liu Peng , Mastromonaco Gabriela , Solmundson Kirsten , Kelly Allicia , Larter Nicholas C. , Gamberg Mary , Schwantje Helen , Thacker Caeley , Polfus Jean , Andrew Leon , Hervieux Dave , Simmons Deborah , Wilson Paul J. 
Published: December 2022

SUMMARYHigh intra-specific genetic diversity is associated with adaptive potential which is key for resilience to global change. However, high variation may also support deleterious alleles through genetic load, unless purged, thereby increasing the risk of inbreeding depression if population sizes decrease rapidly. Purging of deleterious variation has now been demonstrated in some threatened species. However, less is known about the costs of population declines and inbreeding in species with large population sizes and high genetic diversity even though this encompasses many species globally that have or are expected to undergo rapid population declines. Caribou is a species of ecological and cultural significance in North America with a continental-wide distribution supporting extensive phenotypic variation, but with some populations undergoing significant declines resulting in their at-risk status in Canada. We assessed intra-specific genetic variation, adaptive divergence, inbreeding, and genetic load across populations with different demographic histories using an annotated chromosome-scale reference genome and 66 whole genome sequences. We found high genetic diversity and nine phylogenomic lineages across the continent with adaptive diversification of genes, but also high genetic load among lineages. We also found highly divergent levels of inbreeding across individuals including the loss of alleles by drift (genetic erosion) but not purging, likely due to rapid population declines not allowing time for purging of deleterious alleles. As a result, further inbreeding may need to be mitigated through conservation efforts. Our results highlight the ‘double-edged sword’ of genetic diversity that may be representative of other species-at-risk affected by anthropogenic activities.
Combining modelling and experimental approaches to assess the feasibility of developing rice-oil palm agroforestry system
Authors: Perez Raphaël P.A , Vezy Rémi , Bordon Romain , Laisné Thomas , Roques Sandrine , Rebolledo Maria-Camila , Rouan Lauriane , Fabre Denis , Gibert Olivier , De Raissac Marcel 
Published: December 2022

AbstractClimatic hazards affecting the main rice producing regions of Indonesia increase the risk of annual production loss and encourage the development of innovative strategies to maintain stable production. Conversion of oil palm monocultures to rice-based intercropping systems is a strategy to be considered, but relies on the existence of suitable planting management that optimizes both palm productivity while providing enough light for undergrowth rice varieties tolerant to shady conditions. This paper proposes to couple a model of light interception on virtual canopies with indoor experiments to evaluate the feasibility of developing rice-oil palm agroforestry systems. We first selected a planting design that optimized the transmitted light available for rice using a functional-structural plant model (FSPM) of oil palm. Secondly, we reproduced the light regime simulated with specific changes in the intensity and the daily fluctuation of light in controlled conditions. Three light treatments were designed to test independently the effect of daily light quantity and the effect of diurnal fluctuation on contrasted rice subpopulations.Light quantity was the main factor driving changes in plant morphology and architecture, while light fluctuation only appeared to explain variations in yield components and phenology. This study highlighted the importance of light fluctuation in the grain filling process and resource reallocation. The conservation of relative change among varieties between treatments suggests that varietal responses to low light are likely to be heritable, and that varietal screening under full light can provide clue on varietal behavior under low light. However, the identification of specific traits such as a limited expansion of leaf area and a conservation of leaf senescence under shade and high light fluctuation paves the way for selecting varieties dedicated to agroforestry systems. Further investigations including light quality and larger genotypic population to screen are discussed.
DNMT3B PWWP mutations cause hypermethylation of heterochromatin
Authors: Taglini Francesca , Kafetzopoulos Ioannis , Musialik Kamila Irena , Lee Heng Yang , Zhang Yujie , Marenda Mattia , Kerr Lyndsay , Finan Hannah , Rubio-Ramon Cristina , Wapenaar Hannah , Davidson-Smith Hazel , Wills Jimi , Murphy Laura C. , Wheeler Ann , Wilson Marcus D. , Sproul Duncan 
Published: December 2022

AbstractThe correct establishment of DNA methylation patterns is vital for mammalian development and is achieved largely by thede novoDNA methyltransferases DNMT3A and DNMT3B. Mutations in DNMT3B can cause immunodeficiency-centromeric instability-facial anomalies type 1 (ICF1) syndrome which is characterised by hypomethylated heterochromatin. However, in the genome, DNMT3B primarily localises to actively transcribing gene bodies through the interaction of its PWWP domain with the histone modification H3K36me3 and it is unclear how it is recruited to heterochromatin. Here we show that in DNMT3B knockout cells, loss of DNA methylation predominantly occurs in heterochromatic domains marked by H3K9me3. We also find that PWWP domain mutations which disrupt DNMT3B’s interaction with H3K36me3 result in striking increases of DNA methylation in H3K9me3-marked heterochromatin. Gains of methylation are also observed when the PWWP domain of DNMT3B is deleted. In contrast, we find that the ICF1 syndrome-causing PWWP mutation, S270P, does not result in hypermethylation of heterochromatin and destabilises the protein. We also show that removal of the N-terminus region of DNMT3B affects its recruitment to chromatin and ability to methylate H3K9me3 marked regions. Our results suggest that DNMT3B is recruited to H3K9me3 marked heterochromatin in a PWWP-independent manner and that this recruitment is facilitated by the protein’s N-terminus. More generally, we suggest that DNMT3B plays a role in DNA methylation homeostasis at heterochromatin, a process which is disrupted in ICF syndrome, cancer and aging.
Cuticle architecture and mechanical properties: a functional relationship delineated through correlated multimodal imaging
Authors: Reynoud Nicolas , Geneix Nathalie , D’Orlando Angelina , Petit Johann , Mathurin Jeremie , Deniset-Besseau Ariane , Marion Didier , Rothan Christophe , Lahaye Marc , Bakan Bénédicte 
Published: December 2022

AbstractCuticle are multifunctional hydrophobic biocomposites that protect aerial organs of plants. Along plant development, plant cuticle must accommodate different mechanical constraints combining extensibility and stiffness, the corresponding structure-function relationships are unknown. Recent data showed a fine architectural tuning of the cuticle architecture and the corresponding chemical clusters along fruit development which raise the question of their impact on the mechanical properties of the cuticle.We investigated the in-depth nanomechanical properties of tomato fruit cuticle from early development to ripening, in relation to chemical and structural heterogeneities by developing a correlative multimodal imaging approach.Unprecedented sharps heterogeneities were evidenced with the highlighting of an in-depth mechanical gradient and a ‘soft’ central furrow that were maintained throughout the plant development despite the overall increase in elastic modulus. In addition, we demonstrated that these local mechanical areas are correlated to chemical and structural gradients.This study shed light on a fine tuning of mechanical properties of cuticle through the modulation of their architecture, providing new insight for our understanding of structure-function relationships of plant cuticle and for the design of biosinpired material.
Species interactions promote parallel evolution of global transcriptional regulators in a widespreadStaphylococcusspecies
Authors: Cosetta Casey , Niccum Brittany , Kamkari Nick , Dente Michael , Podniesinski Matthew , Wolfe Benjamin E. 
Published: December 2022

ABSTRACTExperimental studies of microbial evolution have largely focused on monocultures of model organisms, but most microbes live in communities where interactions with other species may impact rates and modes of evolution. Using the cheese rind model microbial community, we determined how species interactions shape the evolution of the widespread food- and animal-associated bacteriumStaphylococcus xylosus. We evolvedS. xylosusfor 450 generations alone or in co-culture with one of three microbes: the yeastDebaryomyces hansenii, the bacteriumBrevibacterium aurantiacum, and the moldPenicillium solitum. We used the frequency of colony morphology mutants (pigment and colony texture phenotypes) and whole-genome sequencing of isolates to quantify phenotypic and genomic evolution after 15 weeks of the evolution. The yeastD. hanseniistrongly promoted diversification ofS. xylosus; by the end of the experiment, all populations co-cultured with the yeast were dominated by pigment and colony morphology mutant phenotypes. Populations ofS. xylosusgrown alone, withBrevibacterium, or withPenicilliumdid not evolve novel phenotypic diversity. Whole-genome sequencing of individual mutant isolates across all four treatments revealed numerous unique mutations in the operons for the SigB, Agr, and WalKR global regulators, but only in theD. hanseniitreatment. Phenotyping and RNA-seq experiments demonstrated that these mutations altered pigment and biofilm production, spreading, stress tolerance, and metabolism ofS. xylosus. Fitness experiments revealed trade-offs of these mutations across biotic environments caused by antagonistic pleiotropy, where beneficial mutations that evolved in the presence of the yeastDebaryomyceshad strong negative fitness effects in other biotic environments.IMPORTANCESubstantial phenotypic and genomic variation exists within microbial species, but the ecological factors that shape this strain diversity are poorly characterized. We demonstrate that the biotic context of a widespreadStaphylococcusspecies can impact the evolution of strain diversity. This work demonstrates the potential for microbes in food production environments to rapidly evolve to novel substrates and biotic environments. Our findings may also help understand how otherStaphylococcusspecies may evolve in multispecies microbiomes.
Single nucleus and spatial transcriptomic profiling of human healthy hamstring tendon
Authors: Mimpen Jolet Y. , Ramos-Mucci Lorenzo , Paul Claudia , Kurjan Alina , Hulley Phillipa , Ikwuanusi Chinemerem , Gwilym Steve , Baldwin Mathew J. , Cribbs Adam P. , Snelling Sarah J.B. 
Published: December 2022

AbstractThe molecular and cellular basis of health in human tendons remains poorly understood. Amongst human tendons, the hamstrings are the least likely to be injured or degenerate, providing a prototypic healthy tendon reference. The aim of this study was to define the transcriptome and location of all cell types in healthy hamstring tendon. We profiled the transcriptomes of 10,533 nuclei from 4 healthy donors using single-nucleus RNA sequencing (snRNA-seq) and identified 12 distinct cell types. We confirmed the presence of two fibroblast cell types, endothelial cells, mural cells, and immune cells, and revealed the presence of cell types previously unreported for tendon sites, including different skeletal muscle cell types, satellite cells, adipocytes, and nerve cells, which are undefined nervous system cells. Location of these cell types within tendon was defined using spatial transcriptomics and imaging, and transcriptional networks and cell-cell interactions were identified. We demonstrate that fibroblasts have a high number of potential cell-cell interactions, are present throughout the whole tendon tissue, and play an important role in the production and organisation of extracellular matrix, thus confirming their role as key regulators of hamstring tendon tissue homeostasis. Overall, our findings highlight the highly complex cellular networks underpinning tendon function and underpins the importance of fibroblasts as key regulators of hamstring tendon tissue homeostasis.