bluuebunny/crossref_metadata_2025_split huggingface dataset:
New proapoptotic chemotherapeutic agents based on the quinolone-3-carboxamide scaffold acting by VEGFR-2 inhibition
Authors: El-Fakharany Zeinab S. , Nissan Yassin M. , Sedky Nada K. , Arafa Reem K. , Abou-Seri Sahar M. 
Published: July 2023

AbstractIn the current study, we designed and synthesized a series of new quinoline derivatives 10a-p as antiproliferative agents targeting cancer through inhibition of VEGFR-2. Preliminary molecular docking to assess the interactions of the designed derivatives with the binding site of VEGFR-2 (PDB code: 4ASD) displayed binding poses and interactions comparable to sorafenib. The synthesized compounds exhibited VEGFR-2 inhibitory activity with IC50 ranging from 36 nM to 2.23 μM compared to sorafenib (IC50 = 45 nM), where derivative 10i was the most potent. Additionally, the synthesized derivatives were evaluated in vitro for their cytotoxic activity against HepG2 cancer cell line. Seven compounds 10a, 10c, 10d, 10e, 10i, 10n and 10o (IC50 = 4.60, 4.14, 1.07, 0.88, 1.60, 2.88 and 2.76 μM respectively) displayed better antiproliferative activity than sorafenib (IC50 = 8.38 μM). Compound 10i was tested against Transformed Human Liver Epithelial-2 normal cell line (THLE-2) to evaluate its selective cytotoxicity. Furthermore, 10i, as a potent representative of the series, was assayed for its apoptotic activity and cell cycle kinetics’ influence on HepG2, its effects on the gene expression of VEGFR-2, and protein expression of the apoptotic markers Caspase-7 and Bax. Compound 10i proved to have a potential role in apoptosis by causing significant increase in the early and late apoptotic quartiles, a remarkable activity in elevating the relative protein expression of Bax and Caspase-7 and a significant reduction of VEGFR-2 gene expression. Collectively, the obtained results indicate that compound 10i has a promising potential as a lead compound for the development of new anticancer agents.
Fructooligosaccharide and Bacillus subtilis synbiotic combination promoted disease resistance, but not growth performance, is additive in fish
Authors: Pawar Nilesh Anil , Prakash Chandra , Kohli Mahinder Pal Singh , Jamwal Ankur , Dalvi Rishikesh Subhashrao , Devi B. Nightingale , Singh Soibam Khogen , Gupta Shobha , Lende Smit Ramesh , Sontakke Sadanand D. , Gupta Subodh , Jadhao Sanjay Balkrishna 
Published: July 2023

AbstractSpecies diversification from major to minor carps for their sturdiness and initial higher growth, and also a quest for antibiotic-free aqua farming in the subcontinent, mandates search for and evaluation of alternatives. An experiment was performed to investigate the potential of fructooligosaccharide (FOS) and Bacillus subtilis (BS) (alone or as synbiotics) in promoting growth and immunity against infections in Labeo fimbriatus fingerlings. Six iso-nitrogenous and iso-lipidic diets containing combinations of two levels of FOS (0% and 0.5%) and three levels of BS (0, 104, 106 CFU/g feed) were fed to fish for 60 days. At the end of the feeding trial, twenty-four fish from each group were injected intra-peritoneally with pathogenic strain of Aeromonas hydrophila O:18 to test the immunoprotective efficacy of the supplements against bacterial infection. BS, but not FOS, significantly improved (P < 0.05) growth and feed utilisation attributes like percentage weight gain (PWG), specific growth rate (SGR) and feed conversion ratio (FCR). There were interactive effects of FOS and BS on PWG, SGR and FCR; however, the effects were not additive in nature. These beneficial effects of BS, alone or in combination with FOS, were corroborated by increased protease activity, microvilli density and diameter and number of goblet cells. Overall beneficial effects of FOS and BS included improved erythrocyte (RBC), hemoglobin (Hb), total protein and globulin levels. Total leucocyte (WBC) count and immunological parameters like respiratory burst activity of leucocytes (NBT reduction), lysozyme activity, albumin: globulin ratio and post-challenge survival were significantly improved by both FOS and BS, and their dietary combination yielded the highest improvement in these parameters. Synergistic effects of FOS and BS as dietary supplements indicate that a combination of 106 CFU/g BS and 0.5% FOS is optimal to improve growth, feed utilisation, immune functions, and disease resistance in L. fimbriatus fingerlings.
Active querying approach to epidemic source detection on contact networks
Authors: Sterchi Martin , Hilfiker Lorenz , Grütter Rolf , Bernstein Abraham 
Published: July 2023

AbstractThe problem of identifying the source of an epidemic (also called patient zero) given a network of contacts and a set of infected individuals has attracted interest from a broad range of research communities. The successful and timely identification of the source can prevent a lot of harm as the number of possible infection routes can be narrowed down and potentially infected individuals can be isolated. Previous research on this topic often assumes that it is possible to observe the state of a substantial fraction of individuals in the network before attempting to identify the source. We, on the contrary, assume that observing the state of individuals in the network is costly or difficult and, hence, only the state of one or few individuals is initially observed. Moreover, we presume that not only the source is unknown, but also the duration for which the epidemic has evolved. From this more general problem setting a need to query the state of other (so far unobserved) individuals arises. In analogy with active learning, this leads us to formulate the active querying problem. In the active querying problem, we alternate between a source inference step and a querying step. For the source inference step, we rely on existing work but take a Bayesian perspective by putting a prior on the duration of the epidemic. In the querying step, we aim to query the states of individuals that provide the most information about the source of the epidemic, and to this end, we propose strategies inspired by the active learning literature. Our results are strongly in favor of a querying strategy that selects individuals for whom the disagreement between individual predictions, made by all possible sources separately, and a consensus prediction is maximal. Our approach is flexible and, in particular, can be applied to static as well as temporal networks. To demonstrate our approach’s practical importance, we experiment with three empirical (temporal) contact networks: a network of pig movements, a network of sexual contacts, and a network of face-to-face contacts between residents of a village in Malawi. The results show that active querying strategies can lead to substantially improved source inference results as compared to baseline heuristics. In fact, querying only a small fraction of nodes in a network is often enough to achieve a source inference performance comparable to a situation where the infection states of all nodes are known.
Expectations about system justification predict the ideological gap in attitudes towards immigrants
Authors: Liaquat Usman , Jost John T. 
Published: July 2023

AbstractIn the U.S. political conservatives hold less favorable attitudes than liberals about immigration and immigrant groups. We hypothesized that one reason for this ideological gap is that conservatives are more likely to believe that immigrants are not as justifying of the American system as they should be. This hypothesis was tested in an online study (N = 404) with respect to four immigrant groups: Europeans, East Asians, Middle Easterners, and Latin Americans. Results revealed that conservatism was positively associated with (a) prescriptive beliefs that immigrants should engage in high levels of system justification, and (b) descriptive beliefs that immigrants—except for Middle Eastern immigrants—generally do endorse high levels of system justification. Importantly, conservatives perceived a bigger difference than liberals between prescriptive and descriptive beliefs about immigrants’ system justification levels, and this difference mediated the association between conservatism and attitudes and feelings about non-European (but not European) immigrants. These findings support a new “Perceived System Justification Deficit Model of Prejudice” in which expectations about others’ degree of ideological support for the societal status quo may contribute to out-group bias and perhaps even discrimination.
Isolation and genome characterization of Lloviu virus from Italian Schreibers’s bats
Authors: Tóth Gábor E. , Hume Adam J. , Suder Ellen L. , Zeghbib Safia , Ábrahám Ágota , Lanszki Zsófia , Varga Zsaklin , Tauber Zsófia , Földes Fanni , Zana Brigitta , Scaravelli Dino , Scicluna Maria Teresa , Pereswiet-Soltan Andrea , Görföl Tamás , Terregino Calogero , De Benedictis Paola , Garcia-Dorival Isabel , Alonso Covadonga , Jakab Ferenc , Mühlberger Elke , Leopardi Stefania , Kemenesi Gábor 
Published: July 2023

AbstractLloviu cuevavirus (LLOV) was the first identified member of Filoviridae family outside the Ebola and Marburgvirus genera. A massive die-off of Schreibers’s bats (Miniopterus schreibersii) in the Iberian Peninsula in 2002 led to its initial discovery. Recent studies with recombinant and wild-type LLOV isolates confirmed the zoonotic nature of the virus in vitro. We examined bat samples from Italy for the presence of LLOV in an area outside of the currently known distribution range of the virus. We detected one positive sample from 2020, sequenced the complete coding region of the viral genome and established an infectious isolate of the virus. In addition, we performed the first comprehensive evolutionary analysis of the virus, using the Spanish, Hungarian and the Italian sequences. The most important achievement of this study is the establishment of an additional infectious LLOV isolate from a bat sample using the SuBK12-08 cells, demonstrating that this cell line is highly susceptible to LLOV infection and confirming the previous observation that these bats are effective hosts of the virus in nature. This result further strengthens the role of bats as the natural hosts for zoonotic filoviruses.
Photocatalyzed CO2 reduction to CO by supramolecular photocatalysts made of Ru(II) photosensitizers and Re(I) catalytic subunits containing preformed CO2TEOA adducts
Authors: Santoro Antonio , Cancelliere Ambra M. , Kamogawa Kei , Serroni Scolastica , Puntoriero Fausto , Tamaki Yusuke , Campagna Sebastiano , Ishitani Osamu 
Published: July 2023

AbstractTwo new supramolecular photocatalysts containing Ru(II) polypyridine units as light-harvesting photosensitizers and Re(I) polypyridine subunits as catalytic centers have been prepared. The new species, RuRe2A and Ru2ReA, contain catalytic Re(I) subunits coordinated by the preformed CO2TEOA adduct (known to be the effective catalytic subunits; TEOA is triethanolamine) and exhibit quite efficient and selective photoreduction of CO2 to CO, with outstanding TONs of 2368 and 2695 and a selectivity of 99.9% and 98.9%, respectively. Such photocatalytic properties are significantly improved with respect to those of previously studied RuRe2 and Ru2Re parent compounds, containing chloride ligands instead of the CO2TEOA adduct. Comparison between photocatalytic performance of the new species and their parent compounds allows to investigate the effect of the CO2TEOA insertion process as well as the eventual effect of the presence of chloride ions in solution on the photocatalytic processes. The improved photocatalytic properties of RuRe2A and Ru2ReA compared with their parent species are attributed to a combined effect of different distribution of the one-electron reduced form of the supramolecular photocatalysts on the Ru-subunit(s) (leading to decreased CO formation due to a poisoning ligand loss process) and on the Re-subunit(s) and to the presence of chloride ions in solution for RuRe2 and Ru2Re, which could interfere with the CO2TEOA adduct formation, a needed requisite for CO forming catalysis. These results strongly indicate the utility of preparing supramolecular photocatalysts containing preformed adducts.
Macronutrient distribution in soil subjected to raw sanitary sewage application by closed-end furrows
Authors: Marques Marcus Vinícius Araújo , Silvério Thiago Henrique Ribeiro , Pereira Ana Paula Miranda , von Sperling Marcos , Neves Thiago de Alencar 
Published: July 2023

AbstractThis work aimed to evsaluate the influence of raw sanitary sewage (RS) application in closed-end and level-bottom furrows on the distribution of macronutrients and sodium in the soil along its depth. The applied amount of RS was controlled due to the harmful effect of sodium, with a weekly application over three consecutive years. The experimental design consisted of two experimental plots receiving RS, one with alternating the site of effluent application (alternation of inlet and outlet—TFA) and the other without alternating the site of application (only inlet—TFN); and two experimental plots receiving conventional mineral fertilization, both irrigated with public water supply, where one alternated the site of water application (alternation of inlet and outlet—TWA) and the other without alternating the site of application (only inlet—TWN). The inversion of the flow direction with RS application in the closed-end furrows (TFA) provided a better distribution of nutrients in the soil along its length. There was no significant difference in the levels of macronutrients and sodium between the initial and final regions of the furrow soil. In contrast, the fixed flow direction experimental unit (TFN) exhibit a significant difference between the initial and final levels of total nitrogen, phosphorus, potassium and sodium in the closed furrows with mean concentrations of 265.2, 16.2, 46.7 and 110.0 mg dm−3, respectively.
Change in cooling degree days with global mean temperature rise increasing from 1.5 °C to 2.0 °C
Authors: Miranda Nicole D. , Lizana Jesus , Sparrow Sarah N. , Zachau-Walker Miriam , Watson Peter A. G. , Wallom David C. H. , Khosla Radhika , McCulloch Malcolm 
Published: July 2023

AbstractLimiting global mean temperature rise to 1.5 °C is increasingly out of reach. Here we show the impact on global cooling demand in moving from 1.5 °C to 2.0 °C of global warming. African countries have the highest increase in cooling requirements. Switzerland, the United Kingdom and Norway (traditionally unprepared for heat) will suffer the largest relative cooling demand surges. Immediate and unprecedented adaptation interventions are required worldwide to be prepared for a hotter world.
Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ
Authors: Zhang Yiting , Hu Jianping , Qu Xiaoying , Hu Ke 
Published: July 2023

AbstractDiabetic mellitus-induced diabetic retinopathy is a significant cause of visual impairment and blindness in adults. Circular RNAs (circRNAs) have been shown to play initial roles in vascular progression. However, the mechanism underlying diabetes mellitus-induced vascular complications remains largely unknown. In circRNA chip experiments, circRSU1 was found to be generally overexpressed in diabetic retinopathy patients. Human retina endothelial cells were stably transfected with lentiviruses carrying a circRSU1 interference plasmid. CircRSU1 downregulation alleviated diabetes mellitus induced retina vascular dysfunction, resulting in decreased vascular endothelial growth factor levels, inflammatory responses and oxidative stress. Mechanistically, we showed that elevated circRSU1 expression upregulated the TAZ levels by sponging miR-345-3p. Downregulation of TAZ reversed the vascular dysfunction that was caused by increased circRSU1 expression under hyperglycaemic conditions. In conclusion, overexpression of circRSU1 promotes vascular dysfunction by sponging miR-345-3p to increase the TAZ levels under diabetic conditions. We provide evidence that circRSU1 is a potential therapeutic target for treating diabetes mellitus-induced vascular dysfunction.
Interference, diffraction, and diode effects in superconducting array based on bismuth antimony telluride topological insulator
Authors: Song Xiangyu , Suresh Babu Soorya , Bai Yang , Golubev Dmitry S. , Burkova Irina , Romanov Alexander , Ilin Eduard , Eckstein James N. , Bezryadin Alexey 
Published: July 2023

AbstractIt is well-known in optics that the spectroscopic resolution of a diffraction grating is much better compared to an interference device having just two slits, as in Young’s famous double-slit experiment. On the other hand, it is well known that a classical superconducting quantum interference device (SQUID) is analogous to the optical double-slit experiment. Here we report experiments and present a model describing a superconducting analogue to the diffraction grating, namely an array of superconducting islands positioned on a topological insulator film Bi0.8Sb1.2Te3. In the limit of an extremely weak field, of the order of one vortex per the entire array, such devices exhibit a critical current peak that is much sharper than the analogous peak of an ordinary SQUID. Therefore, such arrays can be used as sensitive absolute magnetic field sensors. A key finding is that the device acts as a superconducting diode, controlled by magnetic field.
Smart cities and the urban digital divide
Authors: Caragliu Andrea , Del Bo Chiara F. 
Published: July 2023

AbstractThe debate on urban smartness as an instrument for managing more efficient cities has been revolving around the notion that Smart Cities might be causing an increase in inequalities. This effect would be caused by the role played in smart urban transformations by Multi-National Corporations, which would be influencing local policymakers’ agendas. In this work we empirically verify whether smart urban characteristics are associated with an increase in urban inequalities along the digital divide dimension among urban dwellers. To this aim, we exploit a large database of 181 European cities, with data on smart urban characteristics, along with measures of the digital divide obtained with the use of survey data carried out at the European Union level. Results show a negative causal relation between the level of urban smartness and the digital divide within-EU cities. Our findings are robust to a number of robustness checks.
Mixed model-based deconvolution of cell-state abundances (MeDuSA) along a one-dimensional trajectory
Authors: Song Liyang , Sun Xiwei , Qi Ting , Yang Jian 
Published: July 2023

AbstractDeconvoluting cell-state abundances from bulk RNA-sequencing data can add considerable value to existing data, but achieving fine-resolution and high-accuracy deconvolution remains a challenge. Here we introduce MeDuSA, a mixed model-based method that leverages single-cell RNA-sequencing data as a reference to estimate cell-state abundances along a one-dimensional trajectory in bulk RNA-sequencing data. The advantage of MeDuSA lies primarily in estimating cell abundance in each state while fitting the remaining cells of the same type individually as random effects. Extensive simulations and real-data benchmark analyses demonstrate that MeDuSA greatly improves the estimation accuracy over existing methods for one-dimensional trajectories. Applying MeDuSA to cohort-level RNA-sequencing datasets reveals associations of cell-state abundances with disease or treatment conditions and cell-state-dependent genetic control of transcription. Our study provides a high-accuracy and fine-resolution method for cell-state deconvolution along a one-dimensional trajectory and demonstrates its utility in characterizing the dynamics of cell states in various biological processes.
Genomic epidemiology of SARS-CoV-2 variants during the first two years of the pandemic in Colombia
Authors: Jimenez-Silva Cinthy , Rivero Ricardo , Douglas Jordan , Bouckaert Remco , Villabona-Arenas Ch. Julian , Atkins Katherine E. , Gastelbondo Bertha , Calderon Alfonso , Guzman Camilo , Echeverri-De la Hoz Daniel , Muñoz Marina , Ballesteros Nathalia , Castañeda Sergio , Patiño Luz H. , Ramirez Angie , Luna Nicolas , Paniz-Mondolfi Alberto , Serrano-Coll Hector , Ramirez Juan David , Mattar Salim , Drummond Alexei J. 
Published: July 2023

AbstractBackgroundThe emergence of highly transmissible SARS-CoV-2 variants has led to surges in cases and the need for global genomic surveillance. While some variants rapidly spread worldwide, other variants only persist nationally. There is a need for more fine-scale analysis to understand transmission dynamics at a country scale. For instance, the Mu variant of interest, also known as lineage B.1.621, was first detected in Colombia and was responsible for a large local wave but only a few sporadic cases elsewhere.MethodsTo better understand the epidemiology of SARS-Cov-2 variants in Colombia, we used 14,049 complete SARS-CoV-2 genomes from the 32 states of Colombia. We performed Bayesian phylodynamic analyses to estimate the time of variants’ introduction, their respective effective reproductive number, and effective population size, and the impact of disease control measures.ResultsHere, we detect a total of 188 SARS-CoV-2 Pango lineages circulating in Colombia since the pandemic’s start. We show that the effective reproduction number oscillated drastically throughout the first two years of the pandemic, with Mu showing the highest transmissibility (Re and growth rate estimation).ConclusionsOur results reinforce that genomic surveillance programs are essential for countries to make evidence-driven interventions toward the emergence and circulation of novel SARS-CoV-2 variants.
Astaxanthin reduces fat storage in a fat-6/fat-7 dependent manner determined using high fat Caenorhabditis elegans
Authors: Xie Junting , Hou Xiaoning , He Wanshi , Xiao Jie , Cao Yong , Liu Xiaojuan 
Published: July 2023

This paper evaluated whether the anti-obesity effect of astaxanthin in Caenorhabditis elegans is related to sbp-1/mdt-15, nuclear hormone and IIS pathway.
Reestablishment of the Smile after Hypoglossal–Facial Nerve Transfer: What Can We Learn?
Authors: Kleijwegt M.C. , Wever C. , Hensen E.F. , Jansen J.C. , Koot R.W. , Malessy M.J.A. 
Published: July 2023

Abstract
Objective The aim of this study was to assess the ability to smile following a hypoglossal–facial nerve transfer (N12–N7).
Design This is a retrospective chart review.
Setting National tertiary referral center for skull base pathology.
Participants Seventeen patients.
Main Outcome Measures The ability to smile following an N12–N7 transfer was assessed by five medical doctors on photographs of the whole face and frontal, orbital, and oral segments. The (segmented) photographs were scored for the symmetry, asymmetry, and correct or incorrect assessment of the affected side.
Results Seventeen patients were analyzed by 5 assessors providing 85 assessments. The whole face at rest was judged symmetrical in 26% of the cases and mildly asymmetrical in 56%. Frontal, orbital, and oral segments were symmetrical in 63, 20, and 35%, respectively. The affected side was correctly identified in 76%. When smiling, the whole face was symmetrical in 6% and mildly asymmetric in 59%. The affected side was correctly identified in 94%. The frontal, orbital, and oral segments during smiling were symmetrical in 67, 15, and 6%, respectively. The affected side of the frontal, orbital, and buccal facial segments during smiling was correctly identified in 89, 89, and 96%, respectively. Interobserver variability with Fleiss' kappa analysis showed that the strength of the agreement during smile of the total face was good (0.771)
Conclusion Following an N12–N7 transfer, a good facial symmetry at rest can be achieved. During smiling, almost all patients showed asymmetry of the face, which was predominantly determined by the orbital and oral segments. To improve the ability to smile after an N12–N7 transfer, additional procedures are needed.