bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poly(ADP-ribosyl)ating enzymes coordinate metabolic rewiring with developmental progression
Authors: Tulin Alexei , Bordet Guillaume , Bamgbose Gbolahan 
Published: September 2023

Abstract
Metabolism, known to be temporally regulated to meet evolving energy demands, plays a crucial role in shaping developmental pace. Therefore, the coordination of developmental progression with metabolism regulation is paramount to ensure appropriate energy production as needed. Recent studies have demonstrated that two key proteins PARP1 and PARG play a regulatory role in the transcription of both morphogenic and metabolic genes. Intriguingly, in Drosophila, the depletion of PARP1 or PARG proteins cause a developmental arrest before pupation, resulting in the accumulation of individuals unable to complete their development. This phenotype highlights the critical involvement of poly(ADP-ribosyl)ating enzymes in regulating the metamorphic process. In this study, we provide compelling evidence that these enzymes intricately coordinate transcriptional changes in both developmental and metabolic pathways during metamorphosis. Specifically, they promote the expression of adult developmental genes crucial for pupation, while simultaneously repressing metabolic genes preceding the transition to the pupal stage. Additionally, these enzymes suppress the expression of larval developmental genes that are no longer required during this transformative period. Our findings shed light on the intricate interplay between poly(ADP-ribosyl)ating enzymes, developmental processes, and metabolic regulation during metamorphosis and highlight a new role of poly(ADP-ribosyl)ating enzymes in the global regulation of transcription.
Development of a relative quantification real-time PCR assay for infection status investigation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis in nursery and finished pigs
Authors: Lin Wei-Hao , Wu Meng-Tien , Yang Cheng-Yao , Lin Chuen-Fu , Lin Chao-Nan , Chiou Ming-Tang 
Published: September 2023

Abstract
Background
Swine enzootic pneumonia (SEP) is an important disease causing severe economic losses in the pig industry. The SEP-like lesions are common in nursery and finishing pigs suffered porcine respiratory disease complex (PRDC) and can be caused by not only Mycoplasma hyopneumoniae (M. hyopneumoniae) but also Mycoplasma hyorhinis (M. hyorhinis). This study aimed to develop a relative quantitative real-time PCR assay to investigate the M. hyopneumoniae and M. hyorhinis load in SEP-like lesions of nursery and slaughter pigs.
Results
The detection limit of the relative quantitative real-time PCR assay was 104 copies/µL. This assay had excellent specificity and showed no cross-reaction with other common swine viral and bacterial pathogens. M. hyorhinis detection rate (63.9%, 133/208) of nursery pigs was significantly higher than M. hyopneumoniae (2.4%), co-infection (24%) (p < 0.0001). The relative quantification ratios of M. hyorhinis in nursery pigs (1.196 ± 0.339) were significantly higher than that of M. hyopneumoniae (0.66 ± 0.26) (p < 0.0001) and that of M. hyorhinis in finishing pigs (0.477 ± 0.17) (p < 0.0001). In contrast, the relative quantification ratios of M. hyopneumoniae in finishing pigs (0.772 ± 0.229) were significantly higher than that of M. hyorhinis (0.477 ± 0.17) (p < 0.0001).
Conclusions
This study firstly developed a M. hyopneumoniae and M. hyorhinis multiplex relative quantification real-time PCR assay to point out the importance of M. hyorhinis as the major pathogen of SEP-like lesion in nursery pigs.
Simulation Tool for Optical Systems: Evaluating and Optimizing Autofocals and Beyond
Authors: Hosp Benedikt , Sauer Yannick , Agarwala Rajat , Wahl Siegfried 
Published: September 2023

Abstract
The development and evaluation of autofocal glasses require robust and efficient tools for assessing their performance in various real-world scenarios. This study presents a novel virtual reality (VR) simulation tool designed to evaluate the control algorithms of autofocal glasses. The tool incorporates a matching task involving Landolt rings and Sloan letters presented on different screens at varying distances and a NASA Task Load Index (TLX) questionnaire to assess participants' subjective workload and convenience. By employing this experimental design, we aimed to gain insights into the effectiveness and efficiency of the evaluated control algorithms for autofocals within our VR-based simulation tool. Our results indicate that the developed VR simulation tool provides a valuable platform for assessing the performance of autofocal glasses in a controlled environment, ultimately contributing to the development of more efficient and user-friendly autofocal glasses.
ToMoDL: A model-based deep learning framework for optical projection tomography
Authors: Obando Marcos , Bassi Andrea , Ducros Nicolas , Mato Germán , Correia Teresa 
Published: September 2023

Abstract
We evaluate a model-based deep learning reconstruction algorithm for optical projection tomography images. The method iterates over a data consistency step and an image domain artefact removal step achieved by a convolutional neural network. A preprocessing stage is also included to avoid potential misalignments between the sample center of rotation and the detector. Our algorithm is trained in a database of wild-type zebrafish (Denio Rerio) at different stages of development to minimise the mean square error for a fixed number of iterations. Using a cross-validation scheme, we compare the results to other methods, such as filtered backprojection, total variation minimization and a direct deep learning (DL) method where the pseudo inverse solution is corrected by a U-Net. We find our method performs equal or better than the alternatives. For a reduced number of projections in comparison to our ground truth, only U-Net is comparable, but we find that our proposal achieves a much better performance if the amount of data available for training is limited, given the much smaller number of parameters our trainable network has.
Climate Change Impact on the Trigger of Natural Disasters Over East Southeastern Himalayas Foothill Region of Myanmar: Extreme Rainfall Analysis
Authors: oo KYAW Than , Haishan CHEN , JONAH Kazora 
Published: September 2023

Abstract
The study examines the characteristics and variability of monsoon rainfall in Myanmar, focusing on the relationship between heavy rainfall, floods, and earthquakes, which impact agriculture, hydrology, and the environment. However, heavy rainfall can cause flooding, economic losses, and water table changes. In northern Myanmar, floods occur mainly during the monsoon season from June to October and can be classified into widespread floods along major rivers like Ayeyarwady, Thanlwin, Chindwin, and Sittoung, and flash floods in small streams and rivers. Climate change is expected to increase the frequency and intensity of extreme weather events, including heavy rainfall, which can trigger floods or landslides, which can in turn cause earthquakes. Heavy rainfall over northern Myanmar and the Sagaing faults, which are the main trigger of earthquakes, has been the subject of several studies. The study uses the Copernicus 5 database of Global Climate Model (GCM) simulations with two scenarios analysis on Climate Change Detection and Indices (ETCCDI) to study changes in climatic extremes. Results show high intensity in the northern region and monsoon core region, while the central region shows less intensity. The study also uses intensity-duration-frequency (IDF) curves to analyze the relationship between rainfall duration, intensity, and return time in major risk zones. The study finds that as short duration lengthens, rainfall intensity increases, while long duration patterns dominate for future rainfall. This information is expected to be convenient for local authorities and flood protection projects in rural and urban basins.
Comprehensive quantum chemical study of the associative complex of para-aminobenzoic acid and 7-diethylamino 4-methyl coumarin by adsorption and aromatic bridges
Authors: Lakhera Shradha , Devlal Kamal , Rana Meenakshi 
Published: September 2023

Abstract
The present study accounts for the quantum chemical and nonlinear optical properties of the combination of para-aminobenzoic acid and 7-diethylamino 4-methyl coumarin. Three different complexes were designed, surface interaction (adsorption) and two by connecting both molecules with π-bridge benzene and biphenyl. The amino and carboxyl groups were observed to behave as strong donor and acceptor sites in all the complexes. The band gap of the adsorbed complex was found more suitable. The absorption wavelength and intensity both were seen to increase with the increase in the number of benzene rings in the π-bridge. The chemical reactivity was established by the global reactivity descriptors. The values of first and second-order hyperpolarizability suggest the improved nonlinear optical responses of the introduced complexes. Additionally, the negative value of second-order hyperpolarizability suggests the possibility of the occurrence of reverse saturable absorption in these combinations. The reported work gives theoretical insights into the nonlinear optical properties of the combination of para-aminobenzoic acid and 7-diethylamino 4-methyl coumarin.
Prevalence and characterization of Leishmania donovani in human hospital cases and domestic dogs in Gadarif State, Sudan
Authors: Abdalla Ahmed Osman Ahmed , Mohammed Abdullah A.A. , hadi Hanan Abdalla Ahmed El , Khaier Mona Abdelrahman Mohamed , Zainaldeen Alsadig Abdalla 
Published: September 2023

Abstract
Background
Leishmania donovani is a protozoan parasite that causes visceral leishmaniasis, a potentially fatal disease in humans and dogs. The epidemiology and transmission dynamics of L. donovani in Sudan are poorly understood.
Objective
To determine the prevalence and characterization of L. donovani in human hospital cases and domestic dogs using different diagnostic methods in two localities in Gadarif State, Sudan.
Material and method:
A cross-sectional study was conducted from October 2019 to April 2020 Whole blood samples and lymph node aspirates were collected from 69 human and 32 dog participants for parasitological, immunological, and molecular tests.
Results
No parasites were detected in blood samples from either humans or dogs. Lymph node microscopy revealed 71.01% positivity in humans and 9.4% in dogs. The IT Leish test showed 82.6% positivity in humans and 43.75% in dogs. PCR confirmed L. donovani infection in all six selected samples (three from humans and three from dogs).
Conclusion
The study confirmed the presence of L. donovani in both human and dog populations in the study area, suggesting that dogs may act as reservoirs or hosts for the parasite. The IT Leish kit test was the most sensitive and specific method, while microscopy of blood and lymph node smears was the least sensitive method. Further studies are needed to elucidate the role of dogs in the transmission cycle of L. donovani and the risk factors associated with human infection.
Clinical impact of spacer placement surgery with expanded polytetrafluoroethylene sheet for particle therapy
Authors: Fujinaka Ryosuke , Komatsu Shohei , Terashima Kazuki , Demizu Yusuke , Omiya Satoshi , Kido Masahiro , Toyama Hirochika , Tokumaru Sunao , Okimoto Tomoaki , Fukumoto Takumi 
Published: September 2023

Abstract
Background: Spacer placement surgery is useful in particle therapy (PT) for patients with abdominopelvic malignant tumors located adjacent to the gastrointestinal tract. This study aimed to assess the safety, efficacy, and long-term outcomes of spacer placement surgery using an expanded polytetrafluoroethylene (ePTFE) spacer.
Methods: This study included 131 patients who underwent ePTFE spacer placement surgery and subsequent PT between September 2006 and June 2019. The overall survival (OS) and local control (LC) rates were calculated using Kaplan-Meier method. Spacer-related complications were classified according to the National Cancer Institute Common Terminology Criteria for Adverse Events (version 5.0).
Results: The median follow-up period after spacer placement surgery was 36.8 months. The 3-year estimated OS and LC rates were 60.5% and 76.5%, respectively. A total of 130 patients (99.2%) were able to complete PT. Spacer-related complications of ≥ grade 3 were observed in four patients (3.1%) in the acute phase and 13 patients (9.9%) in the late phase. Ten patients (7.6%) required removal of the ePTFE spacer.
Conclusions: Spacer placement surgery using an ePTFE spacer for abdominopelvic malignant tumors is technically feasible and acceptable for subsequent PT. However, severe spacer-related late complications were observed in some patients. Since long-term placement of a non-absorbable ePTFE spacer is associated with risks for morbidity and infection, careful long-term follow-up and prompt therapeutic intervention are essential when complications associated with the ePTFE spacer occur.
Trial registration: retrospectively registered
Role of protein phosphatase 2A in inflammation and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis
Authors: Guo Xiaofeng , Bao Guofeng , Zhang Dongmei , Rui Yongjun 
Published: September 2023

Abstract
To understand the effect of protein phosphatase 2A (PP2A) in regulating inflammation and proliferation of fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA), synovial tissues (STs) of osteoarthritis (OA) and RA patients were harvested and FLSs were separated from STs. Several inflammatory factors were applied to stimulate and activate FLSs, which can imitate the process of the inflammatory response in RA FLSs. An inhibitor of PP2A(Okadaic acid) and short interference RNA against PP2A were added to observe that PP2A could influence the inflammation and proliferation of RA FLSs though the ERK and AKT pathways. An agonist of PP2A(FTY720) and lentivirus targeted PP2A were used to investigate that PP2A overexpression could relieve the inflammation and proliferation of RA FLSs. The expression of PP2A and other protein were detected by Western blot and immunohistochemical analysis. The genetic expression of inflammatory factors were measured by real-time polymerase chain reaction. The proliferation of FLSs was detected through the expression of proliferating Cell Nuclear Antigen (PCNA)and cyclin B1. The expression of PP2A in RA STs and FLSs was decreased compared with OA. The level of PP2A was up-regulated in RA FLSs stimulated with TNF-α, IL-1 and lipopolysaccharide(LPS). Inhibition of PP2A by Okadaic acid and special siRNA promoted the inflammation and proliferation of RA FLSs. Furthermore, down-regulation of PP2A increased the phosphorylation level of the ERK and AKT signaling. In addition, FTY720 and lentivirus of PP2A reduced TNF-α, IL-1 and IL-6 production and proliferation of RA FLSs as well as the ERK and AKT pathways. PP2A regulated the inflammatory and proliferation behaviour through the ERK and AKT signaling pathways. Our study suggested that targeting PP2A might a promising therapy to prevent synovial inflammation and destruction.
Channel and Spatial Attention Aware UNet Architecture for Segmentation of Blood Vessels, Exudates and Microaneurysms in Diabetic Retinopathy
Authors: M Anand , A Meenakshi Sundaram 
Published: September 2023

Abstract
Diabetic retinopathy stands out as one of the highly prevalent causes of vision loss in working people worldwide. In computer vision, deep learning based strategies are seen as a viable solution for efficient diabetic retinopathy detection. We present a UNet-based deep learning architecture for diabetic retinopathy segmentation of blood vessels, exudates, and microaneurysms. Traditional methods often consider the features only from the last convolution unit and discard the remaining features, resulting in low-quality feature maps. To overcome this, we introduce a skip connection mechanism to concatenate all attributes from each layer. Additionally, we utilize an upsampling layer to aggregate the features at the final sigmoid layer. Finally, we apply channel and spatial attention mechanisms to generate the semantic feature map. We tested proposed approach on several publicly available datasets such as IDRiD, DIARETDB1, STARE, ChaseDB1, DRIVE, and HRF datasets. The comparative analysis shows that the proposed approach achieves superior performance, with an average accuracy of 98.10%, average sensitivity of 97.60%, and average specificity of 98.2% for segmentation.
Post-fire Behavior of Modified Slurry Infiltrated Fiber Concrete (SIFCON) Plates with Waste Tire Rubber under Flexural Load
Authors: Hashim Ali Mudhafar , Al-Shathr Basil S. 
Published: September 2023

Abstract
In this research, an experimental investigation was conducted on eight modified slurry-infiltrated fiber concrete (SIFCON) one-way plates to examine the performance of SIFCON-containing waste powder rubber as a partial substitute for natural sand (0, 5, 10, and 15%) under two point loads applied at the third portions of the plate after being subjected to a high temperature. The incorporation of rubber particles is primarily motivated by environmental concerns and the desire to reduce the danger of spalling after exposure to elevated temperatures. All adopted specimens had identical dimensions (1000 mm length, 300 mm width, and 50 mm thickness). Four specimens were exposed to fire for two hours at a constant temperature of 600°C, after which they were rapidly cooled by spraying them with water. The flexure behavior of the simply supported plates was evaluated by applying two point loads to the middle third of the plate (a test of four point load). The results revealed that the residual ultimate load of the burned plates with powder rubber replacement percentages of 0, 5, 10, and 15% was 83.3%, 61.08%, 61.03%, and 58.73%, respectively.
Knowledge and Perceptions of the Billing’s Ovulation Method of Natural Family Planning Among Married Women in Lagos, Nigeria
Authors: Abdullateef Ibrahim Bidemi , Abdulganiyu Abdulazeez 
Published: September 2023

Abstract
Background
Based on existing literature, it was observed that modern contraceptives are the most widely used family planning methods in Nigeria, and that they have serious side effects for their users. Despite these effects, women of reproductive age have made little or no effort to embrace Billing's ovulation as an alternative family planning method. In order to better understand the dynamics surrounding the adoption of Billing's ovulation method, this study examined the knowledge, perceived effectiveness, and spousal attitude toward the use of Billing's ovulation as a method of natural family planning among married women in Lagos metropolis.
Method
To accomplish this, a pure qualitative methodological approach was used, with in-depth interviews (IDI) used to collect qualitative data from (20) married women. Manual content analysis was used to analyze the collected data.
Results
Findings showed that; high level of knowledge and awareness of BOM, positive perceptions of its efficacy, and a favorable spousal attitude toward BOM are indicators of the rate at which married women in Lagos metropolis use the Billing's ovulation method as their preferred family planning alternative.
Conclusion
The study then recommends that a rigorous sensitization program based on door-to-door intervention be launched to educate married women in Lagos metropolis on the need for Natural family planning as an alternative family planning method, particularly BOM, while teaching them the procedures and techniques in order to increase people's access to natural family planning.
Immunological aspects of additional revaccination of adolescents against whooping cough, diphtheria and tetanus
Authors: Prutskova E. V. , Cherdantsev A. P. , Kostinov M. P. 
Published: September 2023

The epidemiological situation of whooping cough in many countries of the world shows an increase in the incidence, despite the high coverage of routine immunization of the child population, which indicates the need for additional booster doses in older age groups. Purpose. The study aims at a comparative analysis of the intensity of specific immunity against whooping cough, diphtheria and tetanus in adolescent children after revaccination with different types of vaccines. Material and methods. 121 adolescents received various preparations containing pertussis, diphtheria and tetanus as revaccination. Results. The use of the pertussis component in preparations for revaccination of adolescents, despite the fact that they initially have a certain level of seroprotection, is not accompanied by a significant increase in specific antibody titers. Some differences related to the types of drugs used: the AbDTP-M vaccine against AbDTP had the best seroprotection after 1 month, while at the same time, as after 12 months, this ratio reversed. Conclusion. The situation with the effectiveness of revaccination against tetanus and diphtheria with all the drugs used had a more predictable effect, which was reflected in a significant increase in the titer of specific antibodies after 1 month.
Features of the gut microbiota in children with chronic liver diseases
Authors: Volynets G. V. , Potapov A. S. , Nikitin A. V. , Danilov L. G. , Skvortsova T. A. , Dudurich V. V. 
Published: September 2023

The value of the liver–gut axis is increasingly recognized as a major modulator of autoimmunity. There is no comparative analysis of data on the taxonomic diversity of the intestinal microbiota in chronic liver diseases in children. Purpose. To investigate the taxonomic diversity of the intestinal microbiota in children with chronic liver diseases compared with healthy patients, to identify differences in bacterial diversity in autoimmune and non-autoimmune liver diseases, as well as the impact of immunosuppressive therapy on the intestinal microbiota. Material and methods. A metagenomic analysis of the gut microbiota of 24 children with chronic liver diseases (mean age 10,3 ± 4,7 years) was carried out with the identification of the V3–V4 region of the 16S rRNA gene. The group included 18 children with autoimmune liver diseases and 6 children with non-autoimmune liver diseases. The control group consisted of fecal samples of 34 apparently healthy children. Results. When comparing fecal samples of children with autoimmune liver diseases with samples of healthy children, the taxa of Bacteroides dorei, Collinsella aerofaciens, Ruminococcus caffidurs prevailed, and for children of the control group — Neisseria flavescens. When comparing samples of patients with non-autoimmune liver diseases and the control group, it was found that the taxa Bacteroides fragilis, Klebsiella pneumoniae, Bifidobacterium longum prevailed in healthy children. When comparing fecal samples from children with autoimmune and non-autoimmune liver diseases, it was found that Veillonella dispar, Cloacibacillus porcorum, Veillonella parvula, Prevotella histicola and Bacteroides eggerthii taxa dominate in patients with non-autoimmune diseases. No dominant taxa of the gut microbiota were found in children with autoimmune liver diseases. It has been established that the taxa Veillonella dispar, Faecalibacterium prausnitzii, Roseburia inulinivorans, Bacteroides xylanisolvens and Alistipes obesi prevail in patients receiving immunosuppressive therapy, and the taxa Phascolarctobacterium succinatutens, Bacteroides ovatus, Solobacterium mooreis and Holdemanella massilien prevail in patients not receiving immunosuppressive therapy. Conclusion. A recent study of the gut microbiota in children with chronic liver disease shows differences in the imbalance of the gut microbiota compared to the results obtained in adults. The gut microbiota model is capable of distinguishing autoimmune liver diseases from non-autoimmune diseases. Immunosuppressive therapy is accompanied by the dominance of taxa that reduce the production of short-chain fatty acids.