bluuebunny/crossref_metadata_2025_split huggingface dataset:
Function of Minichromosome maintenance protein 7 (MCM7) in the Brain in a Rat Experimental Subarachnoid Hemorrhage Model
Authors: WANG GUOJUN , zhao li , li zhen , zhu fangxing , ning bo , YUN HUANGHUA , hu quan , zhang shizhong 
Published: March 2023

Abstract
Minichromosome maintenance protein 7 (MCM7), a member belonging to the minichromosome maintenance protein family, takes part in the genome replication regulation in the cell cycle . Although MCM7 expression can be found in almost all tissues, its functions in the central nervous system are still far from full acquaintance. In this research, we utilized an experimental subarachnoid hemorrhage model in adult rats. Sprague dawley (SD) rats were separated into sham groups and SAH groups on 12 h, day 1, day 3, day 5 and day 7 randomly. In SAH groups, 0.3ml atoublood was injected into the prechiasmatic cistern. MCM7 expression is gauged by western blotting, real-time polymerase chain reaction(PCR) and immunofluorescence. The results manifested that the expression of MCM7 went up after SAH, and reached highest level on the third day,going with the up-regulation of Ki67/re-caspase-3 , which displayed highly positive correlation between them. Double staining manifested that the immune response of MCM7 went up mainly in neurons and astrocytes after SAH. Moreover, colocalization of MCM7/Ki-67, MCM7/re-caspase-3 were detected in NeuN, GFAP. In cultured primary astrocytes, knockdown of MCM7 using siRNAs significantly curbed cell proliferation and induced cell apoptosis. In addition, the results manifested that MCM7 may be in connection with cell proliferation and apoptosis after SAH.
The Nucleotide Transformer: Building and Evaluating Robust Foundation Models for Human Genomics
Authors: Lopez Marie , Dalla-Torre Hugo , Gonzalez Liam , Mendoza-Revilla Javier , Carranza Nicolas Lopez , Grzywaczewski Adam , Oteri Francesco , Dallago Christian , Trop Evan , Sirelkhatim Hassan , Richard Guillaume , Skwark Marcin , Beguir Karim , Pierrot Thomas 
Published: March 2023

Abstract

Closing the gap between measurable genetic information and observable traits is a longstanding challenge in genomics. Yet, the prediction of molecular phenotypes from DNA sequences alone remains limited and inaccurate, often driven by the scarcity of annotated data and the inability to transfer learnings between prediction tasks. Here, we present an extensive study of foundation models pre-trained on DNA sequences, named the Nucleotide Transformer, integrating information from 3,202 diverse human genomes, as well as 850 genomes from a wide range of species, including model and non-model organisms. These transformer models yield transferable, context-specific representations of nucleotide sequences, which allow for accurate molecular phenotype prediction even in low-data settings. We show that the sequence representations alone match or outperform specialized methods on 12 of 18 prediction tasks, and up to 15 after fine-tuning. Despite no supervision, the transformer models learned to focus attention on key genomic elements, including those that regulate gene expression, such as enhancers. Lastly, we demonstrate that utilizing model representations can improve the prioritization of functional genetic variants. The training and application of foundational models in genomics explored in this study provide a widely applicable stepping stone to bridge the gap of accurate molecular phenotype prediction from DNA sequence.
Experimental Observation of a Moving Mirror
Authors: Lynch Morgan , Good Michael 
Published: March 2023

Abstract
In this manuscript, we examine the radiative beta decay of free neutrons measured by the RDK II collaboration. The infrared portion of the emitted photon spectrum is shown to corroborate a thermal distribution consistent with the dynamical Casimir effect (DCE). We find the system is thermalized at the local Fulling-Davies-Unruh temperature predicted by the DCE. Substantiated by an excellent chi-squared statistic, we present the evidence for the observation of thermal radiation emitted during the beta decay process in accordance with an accelerated moving mirror.
PACS numbers: 41.60.-m (Radiation by moving charges), 04.70.Dy (Quantum aspects of black holes)
Comparing Irt Properties of Different Likert Scale Lengths: a Case From Attitudinal Measurement on Physics Education Research
Authors: Santoso Purwoko Haryadi , Ismail Raoda , Suhariyono Suhariyono , Setiawati Farida Agus 
Published: March 2023

Abstract
Collapsing Likert scale length on attitudinal measurement toward learning and instruction can be an option by physics education researchers (PER) while interpreting categorical responses from participants. Psychometric evaluation of the different scale length, however, has inconclusive results to date. Item response theory (IRT) offers advanced information at the item level that has been approached in this simulation study to explore psychometric properties of five different Likert scale lengths. The total of 1000 artificial data were generated within five scale variations based on the underlying marginal distribution and inter item correlation matrix. Three parameters (item parameters, global fit indices, and test information functions (TIFs)) from Graded Response Model (GRM) were calculated to compare psychometric behaviors of the observed Likert scale length. Our findings demonstrated the discrimination indices among the five scale lengths remain constant. Global fit indices have no distinguishable information to differentiate the varying points. Based on item endorsement and TIFs, our simulation results confirmed previous works that more information will be raised by the greater number of Likert scale points. The implication intended by this study should provide a key recommendation for a case from attitudinal measurement on the PER community or even beyond the psychometric field.
Indigenous Autism in Canada: A Scoping Review
Authors: Bruno Grant , Chan Titus A , Zwaigenbaum Lonnie , Nicholas David , Coombs Emily 
Published: March 2023

Abstract
Purpose: Currently there is a severe lack of research on autism and Indigenous people in Canada. This scoping review explores explore this literature gap and assesses the same literature from an Indigenous perspective.
Methods: Scoping reviews are an effective means to explore the literature in a specific area, in this case, autism and Indigenous people in Canada. We explored existing literature as it pertains to Indigenous populations and autism in Canada. To support this review, the Indigenous Quality Assessment Tool (QAT) was adapted to appraise the quality of literature.
Results: In total, there were a total of 212 articles identified of which 24 met the inclusion criteria: (1) some focus on autism, (2) a component specific to Indigenous people, and (3) specific to Canada. Of the 24 articles and reports, 15 were peer-reviewed and the rest considered grey literature. Most articles focused on program delivery with some literature using primary data (quantitative and/or qualitative). Overall, the quality of the research was appraised as poor, as determined by the QAT.
Conclusion: Findings reaffirm the critical need for research that addresses autism in Indigenous communities within Canada and show the importance of having research done in full partnership with, or led by, Indigenous people.
Exploration of the optimal pulse oximetry-derived oxygen saturation target for critically ill AECOPD patients: a retrospective cohort study
Authors: Guo Xuequn , Guo Donghao , Luo Qiu 
Published: March 2023

Abstract
Background
Appropriate levels of blood oxygen are crucial for critically ill patients. However, the optimal oxygen saturation has not been confirmed for AECOPD patients during their ICU stays. The purpose of this study was to determine the optimal oxygen saturation range target to reduce mortality for those individuals.
Methods
Data of 533 critically ill AECOPD patients with hypercapnic respiratory failure from the MIMIC-IV database were extracted. The association between median SpO2 value during ICU stay and 30days mortality was analyzed by LOWESS curve, and an optimal range of SpO2(92–96%) platform was observed. Comparisons between subgroups and linear analyses of the percentage of SpO2 in 92–96% and 30days or 180 days mortality were performed to support our view further.
Methods
Although patients with 92–96% SpO2 had a higher rate of invasive ventilator than those with 88–92%, there was no significant increase in the adjusted ICU stay duration, non-invasive ventilator duration, or invasive ventilator duration while leading to lower 30days and 180days mortality in the subgroup with 92–96%. In addition, the percentage of SpO2 in 92–96% was associated with decreased hospital mortality.
Conclusion
In conclusion, SpO2 within 92–96% could lead to lower mortality than 88–92% and > 96% for AECOPD patients during their ICU stay.
Transcriptomic analysis reveals key genes involved in oil accumulation and unsaturated fatty acids biosynthesis in Hibiseu manihot seeds
Authors: Yu Jie , Shi Hui-Min , Xu Hua-Xiang , Guo Xi , Lu Jun-Xing , Zhu Bo , Zhang Tao 
Published: March 2023

Abstract
Background
Hibiseu manihot of various tissues has a high application value, accumulating more than 65% of unsaturated fatty acids in the seeds as well as high levels of oleic acid. Performing transcriptome analysis is beneficial to reveal the molecular mechanism of fatty acid biosynthesis during the development of Hibiseu manihot seeds.
Results
Transcriptome analysis was performed using seeds of Hibiseu manihot at 5, 15 and 30 days after flowering. There were 3338 (1320down, 2018up), 6657 (3408down, 3249up), 3851 (2001down, 1850up) differentially expressed genes in 5 DAF vs 15 DAF, 5 DAF vs 30 DAF, 15 DAF vs 30 DAF. GO functional analysis showed that differentially expressed genes were mainly involved in three functional categories bioengineering, molecular function and cellular component. KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in environmental information processes, genetic information processing, metabolism and other metabolic pathways. Nine genes related to fatty acid biosynthesis and oleic acid metabolic pathways were validated by qRT-PCR, and the results showed that the qRT-PCR were consistent with the transcriptome sequencing results. The analysis of fatty acid content in Hibiseu manihot seeds at different stages showed that oleic acid increased and then decreased with seed development, at the same time, linoleic acid decreased first and then increased. and stearic acid content was more stable. It was hypothesized that KASII, SAD, FATA, PDAT and FAD2 might be closely related to the high content of oleic acid in Hibiseu manihot seeds, which laid a molecular foundation for further study on the functions of KASII, SAD, FATA, PDAT and FAD2.
Conclusions
This study has important significance and reference value for regulating the lipid metabolism pathway of Hibiseu manihot and mining the key genes involved in the lipid synthesis and accumulation of Hibiseu manihot.
Semi-collapse of turbulent fountains in stratified media and the mechanisms to control their dynamics
Authors: Sarasua Luis G. , Caporale Daniel Freire , Barrere Nicasio , Marti Arturo C. 
Published: March 2023

Abstract
Turbulent fountains are widespread natural phenomena with numerous industrial applications. Extensive research has focused on the temporal evolution and maximum height of these fountains, as well as their dependence on Reynolds and Froude numbers. However, the minimum height of the surrounding ambient fluid, which is removed by the fountain due to the entrainment effect, has received little attention. In this study, we investigate the dependence of this minimum height on the characteristics of the fountain and demonstrate how to control it. Our findings present important implications for technological applications of turbulent fountains, particularly in contaminant withdrawal. We discuss the potential of our results to improve the efficiency of such applications.
Does health literacy influence health-related lifestyle behaviors among specialists of health management? A cross-sectional study
Authors: Kinoshita Shunsuke , Hirooka Nobutaka , Kusano Takeru , Saito Kohei , Aoyagi Ryutaro 
Published: March 2023

Abstract
Background: Noncommunicable diseases (NCDs), such as health-related lifestyle diseases, are the leading cause of mortality and societal and economic burdens. Poor lifestyle behaviors, which are modifiable to improve health, can cause diseases, including NCDs. Health literacy has been recognized as an important determinant of health, and studies have shown that higher health literacy is associated with better health outcomes and positive health-related behaviors. However, few studies have investigated the association between health literacy and health-related lifestyle behaviors to understand the mechanistic link between them. Thus, this study investigated the extent to which health literacy at different levels influences health-related lifestyle behaviors among Japanese health professionals, in line with national health promotion.
Methods: A cross-sectional study was conducted among Japanese health management specialists (N = 1,920). Functional, critical, and communicative health literacy were measured. Lifestyle behaviors (exercise, diet and nutrition, sleep, rest, smoking, and alcohol intake), in line with the Japanese National Health Promotion Program, were assessed and calculated into a total cumulative score of health-related lifestyle behaviors. Moreover, we analyzed the associations between the three levels of health literacy and lifestyle behaviors using regression analyses by adjusting for socio-psycho-demographic factors.
Results: Multiple linear regression analyses showed a significant association between the Japanese version of the European Health Literacy Survey Questionnaire and total health-related lifestyle scores (standardized β = 0.160, p< 0.001, R = 0.369) after adjusting for sociodemographic factors. Similarly, the association between communicative and critical health literacy and the total health-related lifestyle scores was significant (standardized β = 0.122, p < 0.001, R = 0.355). The analysis indicated that individuals who had higher level of health literacy (critical and communicative) than functional health literacy (Japanese version of the Newest Vital Sign score) had higher health-related lifestyle behaviors.
Conclusions: A higher level of health literacy is associated with health-related lifestyle behaviors. Health literacy can be a target for interventions to achieve the national goal of lifestyle-related disease prevention and control.
A Novel Energy Optimization System for Renewable Demand using Heuristics, Distributed Systems and Machine Learning in a Smart City
Authors: geo beslin , Pandala Madhavi Latha , n meenakshi , V pandimurugan , jeganathan subash chandra bose 
Published: March 2023

Abstract
Transportation, environmental conditions, quality of human life within smart cities and system infrastructure have all needed practical and dependable smart solutions as urbanisation has accelerated in recent years. In addition, the emerging Internet of Things (IoT) provides access to a plethora of cutting-edge, all-encompassing apps for smart cities, all of which contribute significantly to lowering energy consumption and other negative environmental impacts. For smart cities to meet the challenge of using less energy, the authors of this research article suggest planning and implementing an integrated power and heat architecture that puts renewable energy infrastructure and energy-storage infrastructure at the top of the list. To address these issues, we describe a smart proposed NEOSRD architecture that uses a distributed smart area domain to optimise renewable demand energy in a smart city across a wide area network. The energy requirements of desalination procedures are negligible when compared to the total local energy consumption and transportation, a feat accomplished by the proposed NEOSRD system. Here, the computational model shows how the established system is a valuable response to our problems and a cost-effective strategy for creating the smarter structural elements that cut down on overall smart cities' energy costs.
Isolation of a pair of potent broadly neutralizing mAb binding to RBD and SD1 domains of SARS-CoV-2
Authors: Stuart David , Liu Chang , Das Raksha , Dijokaite-Guraliuc Aiste , Zhou Daming , Duyvesteyn Helen , Mentzer Alexander , Supasa Piyasa , Selvaraj Muneeswaran , Ritter Thomas , Temperton Nigel , Klenerman Paul , Dunachie Susanna , Paterson Neil , Williams Mark , Hall David , Fry Elizabeth , Mongkolsapaya Juthathip , Ren Jingshan , Screaton Gavin 
Published: March 2023

Abstract
Commercially developed monoclonal antibodies (mAb) have been effective in the prevention or treatment of SARS-CoV-2 infection1-3 but the rapid antigenic evolution of the Omicron sub-lineages has reduced their activity4-8 and they are no longer licensed for use in many countries. Here, we isolate spike binding monoclonal antibodies from vaccinees who suffered vaccine break-through infections with Omicron sublineages BA.4/5. We find that it is possible for antibodies targeting highly mutated regions to recover broad activity through allosteric effects (mAb BA.4/5-35) and characterise a pair of potent mAbs with extremely broad neutralization against current and historical SARS-CoV-2 variants. One, mAb BA.4/5-2, binds at the back of the left shoulder of the receptor binding domain (RBD) in an area which has resisted mutational change to date. The second, mAb BA.4/5-5, binds a conserved epitope in sub-domain 1 (SD1). The isolation of this pair of antibodies with non-overlapping epitopes shows that potent and extremely broadly neutralizing antibodies are still generated following infection and SD1 directed mAbs may increase the resilience of mAb therapeutics/prophylactics against SARS-CoV-2.
Efficient C(sp3)–H carbonylation of light and heavy hydrocarbons with carbon monoxide via HAT photocatalysis in flow
Authors: Noel Timothy , Raymenants Fabian , Masson Tom , Orduna Jesus San Jose 
Published: March 2023

Abstract
Despite their abundance in organic molecules, considerable limitations still exist in synthetic methods that target the direct C–H functionalization at sp3-hybridized carbon atoms. This is even more the case for light alkanes, which bear some of the strongest C–H bonds known in Nature, requiring extreme activation conditions that are not tolerant to most organic molecules. To bypass these issues, synthetic chemists rely on prefunctionalized alkyl halides or organometallic coupling partners. However, new synthetic methods that target regioselectively C–H bonds in a variety of different organic scaffolds would be of great added value, not only for the late-stage functionalization of biologically active molecules but also for the catalytic upgrading of cheap and abundant hydrocarbon feedstocks. Here, we describe a general, mild and scalable protocol which enables the direct C(sp3)–H carbonylation of saturated hydrocarbons, including natural products and light alkanes, using photocatalytic hydrogen atom transfer (HAT) and gaseous carbon monoxide (CO). Flow technology was deemed crucial to enable high gas-liquid mass transfer rates and fast reaction kinetics, needed to outpace deleterious reaction pathways, but also to leverage a scalable and safe process.
Screening and Identification of Nanobody against Inhibin α-Subunit from a Camelus Bactrianus Phage Display Library
Authors: Ma Jifu , Khalembek Kezerbek , Xie Jingang , Kadyken Rizabek , Baibatshanov Mukhtar , Jia Haotian , Bodai Bakhet , Kazkhan Oralhazi 
Published: March 2023

Abstract
The inhibin α-subunit is a member of the transforming growth factor family that influences animal reproduction. The purpose of this study was to obtain nanobodies from the phage antibody library constructed by us that can specifically bind to inhibin α-subunit. Such nanobodies can indirectly increase the level of follicle stimulating hormone (FSH) and promote follicular maturation. In this study, Xinjiang Bactrian camel was immunized with prokaryote-expressed inhibin α protein, and the camel phage nanobody display library was prepared by nest polymerase chain reaction (PCR). The nanobodies specifically binding to inhibin α-subunit in the library were screened by three rounds of immunoaffinity screening and phage enzyme-linked immunosorbent assay (phage ELISA). The functions of the selected nanobodies were identified using molecular simulation docking, ELISA affinity test, and sheep immunity test. The results revealed that a nanobody display library was successfully constructed with a capacity of 1.05 × 1012 CFU, and four inhibin-α-subunit-specific nanobodies with an overall similarity of 69.34% were screened from the library, namely, Nb-4, Nb-15, Nb-26, and Nb-57. The results of molecular simulation docking revealed that four types of nanobodies were linked to inhibin-α protein mainly through hydrophobic bonds. Immune tests revealed that the immunity of nanobody Nb-4 could effectively inhibit the level of sheep inhibin A/B, and the nanobody Nb-4 could significantly improve the FSH level in sheep. In summary, four nanobodies with biological function were successfully screened from the constructed antiinhibin α-subunit nanobody library using phage display technique. To the best of our knowledge, this is a new reproductive immunomodulatory pathway of inhibin α-subunit, which may change the secretion of FSH in the ovary, thus changing the estrous cycle of organisms. This study provided a reference for the application of nanobodies in reproductive immunology.
Breast surgery for patients with de novo metastatic breast cancer: A meta-analysis
Authors: Ren Chongxi , Sun Jianna , Kong Lingjun , Wang Hongqiao 
Published: March 2023

Abstract
Background The role of breast surgery in the treatment of patients with de novo metastatic breast cancer (dnMBC) remains controversial, with conflicting trial results. We did a meta-analysis to comprehensively investigate and assess whether breast surgery is associated with survival and quality of life outcomes in patients with dnMBC.
Methods We systematically searched PubMed, Embase, Google Scholar, Scopus, and Web of Science, from database inception to March 30, 2022, for randomised controlled trials (RCTs) that compared breast surgery or locoregional therapy with non-surgical treatment based on systemic therapy for managing dnMBC. We also reviewed abstracts and presentations from major conference proceedings. We excluded non-randomised trials and considered only papers published in English. The primary outcomes were overall survival (OS), locoregional progression-free survival (LPFS), distant progression-free survival (DPFS), and quality of life (QoL).The quality of RCTs was appraised with the Cochrane Collaboration risk of bias tool. Random-effects model was used to calculate the effect sizes of included RCTs.The quality of evidence was evaluated with the Grading of Recommendations Assessment, Development and Evaluation approach. Data analysis was performed with STATA version 17.0 (Statacorp, college station, Tex).
Results A total of 1018 women from seven randomized clinical trials were included in the analysis. Pooled analyses revealed that compared with systemic therapy, breast surgery was not associated with beneficial outcomes in OS (hazard ratio [HR], 0.87; 95% CI, 0.68 to 1.11), DPFS (HR, 0.96; 95% CI, 0.41 to 2.22), or QoL (standardized mean difference [SMD], -0.26; 95% CI,-0.58 to 0.06), but was associated with a benefit in LPFS (HR, 0.36; 95% CI, 0.13 to 0.96).These findings were consistent in subgroup analyses of the timing of surgery, site and number of metastases and tumor molecular subtype.The evidence grade was moderate because of the substantial heterogeneity among studies.
Conclusions Based on the RCTs evidence, we found that breast surgery may benefit locoregional control but does not prolong OS and improve QoL in patients with dnMBC.