bluuebunny/crossref_metadata_2025_split huggingface dataset:
An evidence-informed policymaking (EIPM) competency profile for the Brazilian Health System developed through consensus: process and outcomes
Authors: Barreto Jorge Otávio Maia , Romão Davi Mamblona Marques , Setti Cecília , Machado Maria Lúcia Teixeira , Riera Rachel , Gomes Romeu , Machado Daienne Amaral , Abreu João , de Andrade Keitty Regina Cordeiro , Boeira Laura dos Santos , Pozza Letícia , Souza Nathan Mendes , Logullo Patrícia , Silva Roberta Borges , de Oliveira Sandra Maria do Valle Leone , Mota Sara Emanuela de Carvalho , Dias Tamille Sales , Toma Tereza Setsuko , Silva Silvio Fernandes da 
Published: July 2023

Abstract
Background: Evidence-informed policymaking (EIPM) requires a set of individual and organizational knowledge, skills and attitudes that should be articulated with background factors and needs. In this regard, the development of an EIPM competency profile is important to support the diagnosis, planning and implementation of EIPM.
Purpose: To present the process and outcomes of the development of an EIPM competency profile by an expert committee, to be applied in different contexts of the Brazilian Health System.
Methods: A committee of experts in EIPM shared different views, experiences and opinions to develop an EIPM competency profile for Brazil. In six consensus workshops mediated by facilitators, the committee defined from macro problems to key actions and performances essential for the competency profile. The development steps consisted of: 1) Constitution of the committee, including researchers, professionals with practical experience, managers, and educators; 2) Development of a rapid review on EIPM competency profiles; 3) Agreement on commitments and responsibilities in the processes; 4) Identification and definition of macro problems relating to the scope of the competency profile; and 5) Outlining of general and specific capacities, to be incorporated into the competency profile, categorized by key actions.
Results: The development of the EIPM competency profile was guided by the following macro problems: 1) lack of systematic and transparent decision-making processes in health policy management; 2) underdeveloped institutional capacity for knowledge management and translation; and 3) incipient use of scientific evidence in the formulation and implementation of health policies. A general framework of key actions and performances of the EIPM Competency Profile for Brazil was developed, including 42 specific and general key actions distributed by area of activity (Health Management, Scientific Research, Civil Society, Knowledge Translation, and Cross-sectional areas).
Final considerations: The competency profile presented in this article can be used in different contexts as a key tool for the institutionalization of EIPM.
Validation of Adult Asthma Case Definitions for Primary Care Sentinel Surveillance
Authors: Moloney Max , Morra Alison , Morkem Rachael , Queenan John A. , Gupta Samir , To Teresa , Digby Geneviève , Barber David , Lougheed M Diane 
Published: July 2023

Abstract
Background
Most asthma diagnoses and patient care take place in primary care settings. Electronic medical records (EMRs) offer an opportunity to utilize technology to improve asthma diagnosis and care. The purpose of this study was to create and validate separate case definitions for suspected and confirmed asthma in primary care EMRs, to enable surveillance, benchmarking, and quality improvement in primary care settings.
Objective
To develop a case definition for suspected and confirmed asthma for use in a primary care sentinel surveillance system.
Methods
A single chart abstractor conducted a manual audit of 776 randomly selected patient charts from an academic primary care practice EMR in Kingston, Ontario. Following the single chart abstractor classification, a consensus on chart classification as “not asthma”, “suspected asthma”, or “confirmed asthma” was achieved between the abstractor, a family physician, and a respirologist using Canadian Thoracic Society (CTS) criteria. Case definition algorithms based on billing codes, clinical data elements and medications were applied to the site’s Canadian Primary Care Sentinel Surveillance Network (CPCSSN) data for the same charts and compared to abstractor classifications to determine each algorithm’s measurement properties.
Results
The prevalence of suspected and confirmed asthma were 7.3% (n = 54) and 2.4% (n = 18), respectively. None of the proposed case definitions could differentiate between suspected and confirmed asthma. One algorithm consisting of billing, clinical, and medication elements had the highest Youden’s Index for either suspected or confirmed asthma. The algorithm had a sensitivity of 81%, a specificity of 96%, positive predictive value of 71%, negative predictive value of 98%, and a Youden’s Index of 0.77 for combined suspected or confirmed asthma cases.
Conclusion
An EMR case definition for suspected or confirmed adult asthma has been validated for use in CPCSSN. Implementation of this case definition will enable the development of a surveillance electronic tool (eTool) for adult asthma that can foster quality improvement.
Factors Impacting Clinician Reluctance to Defer Prostate Biopsy in Men with Negative Magnetic Resonance Imaging
Authors: Alwis Samith Minu , Hanna Daniel , Martorana Danielle , Sathiananden Niranjan , Niall Owen , Satasivam Prassannah 
Published: July 2023

Abstract
Purpose: Multiparametric magnetic resonance imaging (mpMRI) enables clinicians to omit prostate biopsy in men with negative (Prostate Imaging-Reporting and Data System v2, PI-RADS ≤ 2) mpMRI. We evaluate one-year outcomes in clinically significant prostate cancer (csPCa) detection following recommendation to defer biopsy in men with negative mpMRI, and assess the relationship between factors influencing adherence to this recommendation and csPCa detection.Methods: Retrospective data including demographics and clinical characteristics were collected for patients who underwent cognitively-targeted transrectal ultrasound-guided biopsy at our institution over July 2017 -June 2020 (Pre-Medicare rebate, Post-Medicare/pre-recommendation and post-recommendation years respectively). Primary endpoint was to determine whether a unit recommendation to only perform biopsy in men with PI-RADS > 2 on mpMRI improved csPCa detection (International Society of Urological Pathology Grade Group, ISUP GG ≥ 2). Secondary endpoint was to assess factors influencing adherence to this recommendation.Results: 130 patients underwent biopsy over the period, with 19 on active surveillance. The recommendation was not adopted and therefore did not improve csPCa detection rate (41% vs 44% pre-recommendation). 52 men proceeded to biopsy despite negative mpMRI, a greater number than pre-recommendation (n = 35, 37%). Eight men with a negative mpMRI had csPCa, with two reporting high-grade disease (ISUP GG ≥ 4). On multivariable analysis, age, prostate-specific antigen, prostate volume, number of cores, abnormal digital rectal examination and family history were not associated with csPCa detection in men with negative mpMRI.Conclusion: The recommendation to defer biopsy in men with negative MRI was not adopted by clinicians. Although patient and clinical factors were commonly quoted reasons to biopsy, none were found to be accurate predictors of csPCa.
The Prediction through Artificial Intelligence Approach and Geographic Information Systems (GIS) of the Soil Temperature in Kastamonu Province, Turkey
Authors: Gürdal Mehmet 
Published: July 2023

Abstract

In the present work, the average soil temperature of Kastamonu province was predicted by artificial neural networks approach employing data gained from five various meteorological measurement districts located in provincial borders. Twenty-two years of (2000–2021) monthly average atmosphere temperature data achieved from soil depths (5, 10, 20, 50, and 100 cm) have been utilized for artificial intelligence structure. It has been compared monthly average soil temperature for Cide, Devrekani, İnebolu, Kastamonu City Center, and Tosya stations. Measured and estimated soil temperature values have been exceedingly related to the Correlation Coefficient values (R2), Mean Absolute Error (MAE), Mean Square Error (MSE), and Average Relative Deviation (ARD). As a result, the estimated soil temperature findings were in the acceptable range with the measured data with average R2 values of 0.9851, 0.9456, 0.9712, 0.9691, and 0.9586 for Cide, Devrekani, İnebolu, Kastamonu CC, and Tosya, the respectively. MAE of 0.6808°C to 0.6848°C, ARD of 0.010–10.674% and MSE of 0.144 and 4.109 at all measurement districts where insignificant error tendency is very clear.
Analysis of the characteristics, efficiency, and influencing factors of third-party mediation mechanisms for resolving medical disputes in public hospitals in China using 5,948 cases
Authors: Shen Yanfei , Li Gaiyun , Tang Zhiguo , Wang Qi , Zhang Zurong , Hao Xiangyong , Han Xuemei 
Published: July 2023

Abstract
Background:
Medical disputes, which are prevalent in China, are a growing global public health problem. The Chinese government has proposed third-party mediation (TPM) to resolve this issue. However, the characteristics, efficiency, and influencing factors of TPM in resolving medical disputes in public hospitals in China have yet to be determined.
Methods:
We conducted a systematic study using TPM records from medical disputes in Gansu Province in China from 2014 to 2019. A χ2 test was used to compare differences between groups, and binary logistic analysis was performed to determine the factors influencing the choice of TPM for resolving medical disputes.
Results:
We analyzed 5,948 TPM records of medical disputes in Gansu Province in China. The number of medical disputes and the amount of compensation awarded in public hospitals in the Gansu Province increased annually from 2014 to 2019, with most of the disputes occurring in secondary and tertiary hospitals. Approximately 89.01% of the medical disputes were handled by TPM; the average compensation amount with TPM was Chinese Yuan (CNY) 48,688.73, significantly less than that awarded via court judgment and judicial mediation. Moreover, compensation amounts, non-fatal outcomes, and no-fault liability determinations influence the choice of TPM for settling medical disputes.
Conclusion:
Our findings demonstrate that TPM mechanisms play a positive role in efficiently reducing compensation amounts and increasing medical dispute resolution rates.
Multi-ancestry epigenome-wide analyses identify methylated sites associated with aortic augmentation index in TOPMed MESA
Authors: Manichaikul Ani , Hu Xiaowei , Logan Jeongok , Kwon Younghoon , Lima Joao , Jacobs David , Duprez Daniel , Brumback Lyndia , Taylor Kent , Durda Peter , Johnson Craig , Cornell Elaine , Guo Xiuqing , Liu Yongmei , Tracy Russell , Blackwell Thomas , Papanicolaou George , Mitchell Gary , Rich Stephen , Rotter Jerome , Berg David Van Den , Chirinos Julio , Hughes Timothy , Garrett-Bakelman Francine 
Published: July 2023

Abstract
Despite the prognostic value of arterial stiffness (AS) and pulsatile hemodynamics (PH) for cardiovascular morbidity and mortality, epigenetic modifications that contribute to AS/PH remain unknown. To gain a better understanding of the link between epigenetics (DNA methylation) and AS/PH, we examined the relationship of eight measures of AS/PH with CpG sites and co-methylated regions using multi-ancestry participants from Trans-Omics for Precision Medicine (TOPMed) Multi-Ethnic Study of Atherosclerosis (MESA) with sample sizes ranging from 438 to 874. Epigenome-wide association analysis identified one genome-wide significant CpG (cg20711926-CYP1B1) associated with aortic augmentation index (AIx). Follow-up analyses, including gene set enrichment analysis, expression quantitative trait methylation analysis, and functional enrichment analysis on differentially methylated positions and regions, further prioritized three CpGs and their annotated genes (cg23800023-ETS1, cg08426368-TGFB3, and cg17350632-HLA-DPB1) for AIx. Among these, ETS1 and TGFB3 have been previously prioritized as candidate genes. Furthermore, both ETS1 and HLA-DPB1 have significant tissue correlations between Whole Blood and Aorta in GTEx, which suggests ETS1 and HLA-DPB1 could be potential biomarkers in understanding pathophysiology of AS/PH. Overall, our findings support the possible role of epigenetic regulation via DNA methylation of specific genes associated with AIx as well as identifying potential targets for regulation of AS/PH.
Oxidative photocatalysis on membranes triggers non-canonical pyroptosis
Authors: Kwon Tae-Hyuk , Lee Chaiheon , Park Mingyu , Wijesinghe W.C. Bhashini , Lee Chae Gyu , Hwang Eunhye , Yoon Gwangsu , Lee Jeong Kyeong , Roh Deok-Ho , Kwon Yoon Hee , Yang Jihyeon , Hughes Sebastian A. , Vince James , Seo Jeong Kon , Min Duyoung 
Published: July 2023

Abstract

Intracellular membranes composing organelles of eukaryotes include membrane proteins playing crucial roles in physiological functions. However, a comprehensive understanding of the cellular responses triggered by intracellular membrane-targeted oxidative stress remains elusive. Herein, we developed an amphiphilic photocatalyst localised in intracellular membranes to damage membrane proteins oxidatively, resulting in non-canonical pyroptosis. Our developed photocatalysis generated hydroxyl radicals and hydrogen peroxides via water oxidation, which was accelerated under hypoxia. Single-molecule magnetic tweezers revealed that photocatalysis-induced oxidation markedly destabilised membrane protein folding. In cell environment, label-free quantification revealed that oxidative damage occurred primarily in membrane proteins related to protein quality control, thereby aggravating mitochondrial and endoplasmic reticulum stress and inducing lytic cell death. Notably, the photocatalysis activated non-canonical inflammasome caspases, resulting in gasdermin D cleavage to its pore-forming fragment and subsequent pyroptosis. These findings suggest that the oxidation of intracellular membrane proteins triggers non-canonical pyroptosis.
Antcin-B, a phytosterol-like compound from Taiwanofungus camphoratus inhibits SARS-CoV-2 3-chymotrypsin-like protease (3CL Pro ) activity in-silico and in vitro
Authors: Dakpa Gyaltsen , Kumar K. J. Senthil , Nelen Jochem , Pérez-Sánchez Horacio , Wang Sheng-Yang 
Published: July 2023

Abstract
Antcins obtained from the fruiting bodies of Taiwanofungus camphoratus, have been traditionally used to treat infections, whereas their role in the SARS-CoV-2 3-chymotrypsin-like protease (3CLPro) remains unclear. We employed both in vitro and in silico approaches to understand the underlying chemical mechanism comprehensively. Our finding revealed that various antcins (A, B, C, H, I, and M) and non-antcins (citronellol and limonene) exhibited lower toxicity to cells than GC376, along with favorable drug-likeness based MTT assay and ADMET prediction. Molecular docking analysis predicted that antcin-B possessed the lowest binding affinity energy and interacts with key residues such as Glu169, Gln189, His41, Leu141, Asn142, Glu16, and His165 employing hydrophobic interaction, hydrogen bonding, and salt bridge. These interactions were further confirmed by molecular dynamics (MD) simulation, which demonstrated the formation of hydrogen bonds with Asn142 and Gly143 and bridge with Glu166 for approximately 40% and 70% of the simulation time, respectively. Indeed, in vitro, 3CLPro activity analysis supports the above notion that antcin-B significantly (96.39%) inhibits 3CLPro activity, which is highly comparable with a known antiviral drug GC367 (96.72%). Consequently, antcin-B could be considered for developing a potential drug candidate for inhibiting 3CLPro activity, thereby impeding reducing the severity of COVID-19 in patients.
BRCA1 and BRCA2 gene mutation spectrum and high frequency of BRCA1 185delAG among breast and ovarian cancer patients from Southern India.
Authors: Chapla Aaron , John Ajoy , Singh Ashish , Yadav Pratibha , Joel Anjana , Thumaty Divya , K Fibi , Georgy Josh , Cherian Anish , Thomas Shawn , Thomas Anitha , Thomas Vinotha , Peedicayil Abraham , Varghese Deny , R Parthiban , Ravichandran Lavanya , Johnson Jabasteen , Thomas Nihal , Yadav Bijesh , S Patricia , B Selvamani , Abraham Deepak , Paul M , Titus Raju 
Published: July 2023

Abstract
In a developing country like India, genomic data sets for even the most clinically relevant genes like BRCA1 and BRCA2 are relatively scarce. There is also a need to identify and screen population specific BRCA hotspot mutations to pave the way for affordable genetic testing strategies in clinical practice. We have carried out an ambispective study to evaluate Next-generations Sequencing (NGS) based approach to identify pathogenic variants in BRCA1 and BRCA2 genes among 772 breast and ovarian cancer patients. The target enrichment was carried out using the in-house designed Multiplex-PCR for BRCA1 and BRCA2, followed by targeted NGS on Ion Torrent Personal Genome Machine. Additionally, allele-specific PCR (ASPCR) based genotyping of BRCA1 c.68_69delAG also known as 185delAG, was carried out in 149 patients. We identified 181 BRCA1 and BRCA2 variants, and based on ACMG 2015 guidelines, these variants were classified as 111 pathogenic or likely pathogenic and 70 VUS (Variant with uncertain significance). The 185delAG was identified as a recurrent mutation in the Southern Indian population accounting for 25.21% of the pathogenic variants. In addition, a family history of cancers of the breast, ovary, pancreas, or prostate (BOPP) was found to be associated with a higher risk of identifying a deleterious BRCA1/2 variant [OR=2.15 (95%CI 1.46-3.2) p≤0.0001]. These results suggest that Multiplex PCR coupled NGS is a sensitive and specific strategy for BRCA testing. However, ASPCR-based genotyping of 185delAG followed by targeted NGS would be cost-effective in South Indian patients.
Path analysis of influencing factors for hoarding among college Students
Authors: Li Wangyue , Liu Na , Pei Tao , Chen Haocheng , Xie Minyao , Song Shasha , Zhang Xuedi , Jiang wenjing , Hong Can , Zhang Ning 
Published: July 2023

Abstract
Background: Hoarding may affect an individual’s daily life and mental health, moreover, it can gradually attract social attention and become a public health problem. Therefore, we explored the hoarding phenomenon and its influencing factors among college students in China to better understand the current status quo and how it is related to mental health issues. In addition, we describe appropriate interventions that could reduce the impact of hoarding.
Methods: This cross-sectional study (N=3958) was conducted from September 2020 to December 2020. An online survey was used to collect demographic and psychological data about depression, compulsion and hoarding.
Results: Of the 3958 students who completed the survey, 178 (4.5%) exhibited hoarding behaviors, and the results of the path analysis showed that life events and neurotic personality traits can influence hoarding directly and indirectly through compulsions.
Conclusion: Obsessive-compulsive symptom plays a mediating role in the relationship between life events and neurotic personality traits.
Optimization Pilot Scale Study on Ammonia Nitrogen Removal by Bio Filter
Authors: Meng Junlong , Shen Songtao , Zhou Chuanhui , Zhang Tuodi , Xu Yingyi 
Published: July 2023

Abstract
In order to obtain the optimal conditions for ammonia nitrogen (AN) wastewater treatment by bio filter (BF), the effects of C/N, pH, and hydraulic load (HL) on the AN degradation were studied by Response Surface Methodology (RSM). Central Composite Design (CCD) experiments were conducted, and the response of the AN removal rates were fitted to a second-order polynomial model. The analysis of variance showed that the model was accurate and reliable. Through model fitting, the optimal condition for AN removal was: C/N of 18.95, pH of 7.78, and HL of 1.04 d-1. The maximum AN removal rate predicted by the model was 91.90%, accorded with the experimental verification value of 91.37% under the optimal condition. The research provided valuable demonstration for optimizing process parameters on AN removal in BF.
Spatiotemporal and Seasonal Transmission Dynamics of Schistosoma haematobium and Snail Infectivity in Ase River Catchment, Southern Nigeria
Authors: Ito Edore Edwin , Nduka F.O , Eze C.N 
Published: July 2023

Abstract
Bulinus are intermediate snail hosts of Schistosoma haematobium. Despite their vectorial role, the transmission dynamics and infectivity of these intermediate snail hosts remain understudied in the Ase River. This longitudinal study evaluated the geospatial and seasonal transmission patterns and infectivity of three S. haematobium vectors between November 2020 and October 2022 in the Ase River catchment, Delta State, Nigeria. Eleven (11) geospatial water contact coordinates were mapped for monthly spatiotemporal collection of Bulinus species along the Ase River and its catchment, for two years. Snail sampling was performed for 45 minutes at each study site using scooping/hand-picking techniques and subsequently counted, identified, and recorded. Snails of the Bulinus genus were individually placed in a beaker containing distilled water and exposed to light to shed cercariae which were identified to be human schistosome type. The number of infected snails for each month and the season was also counted and recorded to establish spatiotemporal and seasonal infectivity transmission dynamics and infectivity. A total of 41.45% of the 2345 Bulinus snails collected were infected with S. haematobium. The monthly infectivity of Bulinus snails varied significantly (P < 0.05) throughout the study period (P = < 0.0001; F = 23.11; df = 11). Further analysis showed a strong significant association (χ2 = 23.57; df = 11; p = 0.015) between the study years. The Principal Component Analysis (PCA) also indicates that Bulinus infectivity in the Ase River catchment was primarily driven by February and January. B. truncatus consistently had the highest transmission potential, followed by B. globosus and B. senegalensis. ANOVA confirms that the monthly/study site infectivity and transmission potential in B. truncates, B. globosus and S. senegalensis were statistically, significant (P < 0.05). These results demonstrated a clear distinction in the patterns and relationships between the different months in terms of snail infectivity and seasonal transmission potential. This understanding will help in the continuous monitoring and targeted interventions to control schistosomiasis transmission in Ase River.
Comparison of Laryngeal Mask Airway and Endotracheal Tube Placement in Neonates
Authors: Roberts Kari , Wanous Amanda , Brown Roland , Rudser Kyle 
Published: July 2023

Abstract
Objective
We hypothesize that the time, number of attempts and physiologic stability of placement of an LMA would be superior compared to ETT.
Study Design:
Videotape and physiologic parameters of LMA (n = 36) and ETT (n = 31) placement procedures for infants 28–36 weeks gestation were reviewed.
Results
Duration of attempts (32 vs 66 sec, p < 0.001) and mean total procedure time (88 vs 153 sec, p = 0.06) was shorter for LMA compared to ETT. Mean number of attempts for successful placement was fewer for LMA (1.5 vs 1.9, p = 0.11). Physiologic parameters remained near baseline in both groups despite very different degrees of premedication.
Conclusion
Placement of an LMA required less time and fewer number of attempts compared to ETT. Physiologic stability of an LMA was maintained without the use of an analgesic and muscle relaxant. Use of an LMA is a favorable alternative to ETT placement for surfactant delivery in neonates.
Trial Registration
NCT01116921