bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of cognitive impairment on Health-Related Quality of Life in Systemic Lupus Erythematosus: a cross-sectional study
Authors: Costanzo Giulia , Lorefice Lorena , Firinu Davide , Carta Elisa , Murgia Giuseppe , Messina Maria Rita , Torrazza Mario , Locci Carlotta , Barca Maria Pina , Giacco Stefano Del , Cocco Eleonora , Fenu Giuseppe 
Published: February 2023

Abstract
Purpose: To correlate the cognitive functions evaluated by the Symbol Digit Modalities Test (SDMT)with the principal clinical features and Health-related Quality of Life (HRQoL) measures of SLE.
Material and methods: We recruited consecutive outpatients with SLE from a third-level Lupus Clinic in Italy to undergo neuropsychological evaluation using SDMT and the LupusQoL-IT as HRQoL questionnaire. Patients were defined as either neuropsychiatric-SLE (NPSLE) or as non-neuropsychiatric-SLE (non-NPSLE); they were also classified as cognitive-impaired and cognitive-preserved based on SDMT score alteration corrected for age, gender and education.
In this cross-sectional study we included and studied 140 consecutive patients; 30 patients had NPSLE. Inclusion criteria were 4 or more ACR criteria for SLE, exclusion criteria were non–italian speaking, ocular disease interfering with reading capacity, the onset of any major medical condition (e.g. requiring hospitalization) or overt new neuropsychiatric event within the study period.
Results: The mean SDMT score was 41.4±12.8. Alteration of SDMT score was detected in 29.9% of patients. Pearson test showed a correlation between SDMT score and Systemic Lupus International Collaborating Clinics/ACR Damage Index (SLICC/ACR-SDI) score (r:-0.373, p< 0.001) and specific HRQoL domains. Cognitive-impaired and cognitive-preserved patients showed a significant difference in physical health (p=0.03), pain (p=0.04) and planning (p=0.02) domains of the HRQol. A multiple linear regression (dependent variable: SDMT score) revealed a relationship with SLICC/ACR-SDI (p=0.005) and with other clinical features, after adjustment. SDMT score was significantly more frequently altered among patients with NPSLE (p=0.009) and the mean was significantly different in comparison to non-NPSLE patients (p=0.01).
Conclusions: Our data demonstrate the correlation between cognitive impairment captured using SDMT and HRQoL of SLE patients and show a correlation of measures such as SLICC/ACR-SDI with SDMT score.
Bifactor analysis of the Hospital Anxiety and Depression Scale (HADS) for individuals with traumatic brain injury
Authors: Carmichael Jai , Spitz Gershon , Gould Kate Rachel , Johnston Lisa , Samiotis Alexia , Ponsford Jennie 
Published: February 2023

Abstract
Anxiety and depression are two of the most common forms of psychopathology experienced after traumatic brain injury (TBI), yet there is a scarcity of studies validating measures of anxiety and depression for use with this population. Using symmetrical bifactor modeling, we evaluated whether the Hospital Anxiety and Depression Scale (HADS) reliably differentiated anxiety and depression in 874 adults with moderate-severe TBI. There was a dominant general distress factor accounting for 84% of the systematic variance in the HADS total score. The specific anxiety and depression factors accounted for little residual variance in their respective subscale scores (12% and 20%, respectively), and overall, minimal bias was found in using the HADS as a unidimensional measure. Further, in a subsample (n=184), the HADS subscales did not clearly discriminate between formal anxiety and depressive disorders diagnosed via semi-structured clinical interview. Results were consistent when accounting for degree of disability, non-English speaking background, and time post-injury. In conclusion, variance in HADS scores after TBI predominately reflects a single underlying latent variable. Clinicians and researchers working with individuals with TBI should exercise caution in interpreting the individual HADS subscales, instead considering using the total score as a more valid measure of general distress.
Application of a self-developed, low-budget indocyanine green camera in surgical imaging – a single institution's experiences
Authors: Sandor Zoltan , Ujfalusi Zoltan , Varga Adam 
Published: February 2023

Abstract
Introduction: Indocyanine green is a fluorescent dye, the use of which is becoming more and more widespread in many areas of surgery. Several international studies deal with the dye’s effectiveness in intraoperative angiography, the search for tumors, the more precise identification of anatomical structures, the detection of lymph nodes and lymph ducts, etc. The application of the dye is safe, but a suitable equipment park is required for its use, which entails relatively high costs.
Objectives: The aim of our research is to create a detector system on a low budget, able to be used safely in everyday practice and to illustrate its operation with practical examples in our own institute.
Methods: By modifying a web camera and using filter lenses and special LEDs, we created a device suitable for exciting and detecting indocyanine green fluorescence. We prove its excellent usability during the following surgeries at our institute: breast tumor surgery, kidney transplantation, bowel resection, parathyroid surgery and liver tumor resection.
Results: A low budget, easy-to-use tool was created, which is suitable for taking advantage of the opportunities provided by indocyanine green.
Engineered Antiviral Sensor Targets Infected Mosquitoes
Authors: Benetta Elena Dalla , López-Denman Adam , Li Hsing-Han , Masri Reem , Brogan Daniel , Bui Michelle , Yang Ting , Li Ming , Dunn Michael , Klein Melissa , Jackson Sarah , Catalan Kyle , Blasdell Kim , Tng Priscilla , antoshechkin igor , Alphey Luke , Paradkar Prasad , Akbari Omar 
Published: February 2023

AbstractEscalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (vRNAExpressionActivatesPoisonousEffectorRibonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR Cas13 and its potent collateral activity. Akin to a stealthy Trojan Horse hiding in stealth awaiting the presence of its enemy, REAPER remains concealed within the mosquito until an infectious blood meal is uptaken. Upon target viral RNA infection, REAPER activates, triggering programmed destruction of its target arbovirus such as chikungunya. Consequently, Cas13 mediated RNA targeting significantly reduces viral replication and its promiscuous collateral activity can even kill infected mosquitoes. This innovative REAPER technology adds to an arsenal of effective molecular genetic tools to combat mosquito virus transmission.
Dummy Variable Regression and Artificial Neural Networks for Maize Grain Yields Prediction in Push-Pull Systems
Authors: Guera Ouorou Ganni Mariel , Castrejón-Ayala Federico , Robledo Norma , Jiménez-Pérez Alfredo , Salazar-Marcial Lilia 
Published: February 2023

Abstract
Crop yield prediction is essential for sustainable production planning. In agroecological systems, the traditional linear or non-linear regression models used for this purpose present limitations and robustness problems due to the number of variables generated by the complexity of these systems. Therefore, the present study was carried out with the objectives of 1) fitting multiple linear regression models with dummy variables using the Ordinary Least Squares method (OLS); 2) parameterizing and training Artificial Neural Networks (ANNs) with the backpropagation algorithm; and 3) comparing the performance of both approaches in maize yield prediction in push-pull systems established in Yautepec, Morelos, Mexico. In both modeling approaches, maize grain yield predictive variables were: edaphoclimatic (soil temperature and moisture), phytosanitary (incidence and severity of Spodoptera frugiperda), morphological (leaf area index), and categorical (Blocks, Management Systems) variables. The ANN of architecture MLP 18-13-1 (r = 0.95; RMSE = 12.19%), with hyperbolic tangent activation function in the hidden layer and linear function in the output layer, generated consistent and more accurate predictions than those obtained with the regression equation with dummy variables (r = 0.87; R2 = 0.75; RMSE = 20.38%).
Evidence of philopatry in white mullet Mugil curema (Pisces: Mugilidae)
Authors: Colín Angélica , Castañeda-Rico Susette , Guevara-Chumacero Luis M. , Pacheco-Almanzar Eloisa L , Ibáñez Ana Laura 
Published: February 2023

Abstract
Mugil curema is a common species in coastal areas to which is conferred reproductive migrations to the open sea with possibilities to spawn in estuarine waters. Previous studies described it as a species complex, which could be explained in terms of reproductive isolation and/or philopatric behavior. In order to test philopatry in M. curema, 10 locations in the Gulf of Mexico, one in the Atlantic Ocean, and three in the Mexican Pacific were sampled. One hundred individuals were analyzed with Cytochrome Oxidase Subunit I (COI), 121 with control region, and 380 with microsatellite loci. The mitochondrial results showed two lineages: Gulf of Mexico (GOM) and Mexican Pacific (MP) separated by a genetic distance of 4.5% with COI and 11.7% with control region. However, shared haplotypes were found between oceans, suggesting evidence of an ancestral connection. In addition, genetic structure was found in MP, suggesting the possibility of cryptic lineages. The microsatellite results showed genetic connection between GOM and MP probably as a consequence of a recent contact. It also showed genetic structure within GOM and MP; genetic subgroups were different by sex for the GOM (FST=0.348 ♂ and 0.275 ♀) and similar for the MP (FST=0.233 ♂ and 0.166 ♀). Based on the results of genetic structure and gene flow the hypothesis of sex-specific philopatry in GOM (♂ philopatric) and both sexes in MP is supported. The divergence found between GOM and MP, and the different philopatric behavior support the hypothesis that they may represent two distinct lineages.
Can a Model With Prescribed Cloud Droplet Number Concentrations Simulate the Outcomes of Aerosol-Cloud Interactions Like a Chemistry Model?
Authors: Takeishi Azusa , Wang Chien 
Published: February 2023

Abstract
With the general rise of computational capacities and the continued efforts to improve computational efficiencies, more and more studies have been utilizing state-of-the-art atmospheric models that enable cloud-resolving simulations over a global domain. Microphysical processes inside clouds, however, are on a scale much smaller than that of a cloud itself, and therefore resolving clouds in a model is not equivalent to resolving cloud microphysical processes. When aerosol-cloud interaction (ACI) is studied, chemistry models enable the prognostic calculations of chemical species, including aerosols, which can perturb cloud microphysics and eventually impact clouds and climate. The large drawback of these models is the high computational cost required for tracking chemical species in space and time that may not be affordable in some studies. As a result, some studies have used non-chemistry models with prescribed cloud droplet number concentrations nc and compared multiple simulations with different nc to assess the impacts of varying aerosol concentrations on clouds. In this study we assess whether the same or similar ACI can be simulated when the aerosol number is increased in a chemistry model and when nc is raised in a non-chemistry model. A case study has been conducted over the Maritime Continent in September 2015 when an extremely large number of aerosols were observed due to fires under a dry condition brought by a strong El Niño. The comparison of the simulations by the chemistry and non-chemistry models shows that the aerosol-driven enhancement of rainfall in the chemistry simulations was not present in the non-chemistry simulations, even with prescribed nc with certain spatial variation based on the chemistry runs. Therefore, simulated ACI may largely differ depending on the manner by which an increase or decrease of aerosols is represented in a model. The result suggests the further need for powerful computational capabilities and the pursuit of a rigorous means to incorporate aerosol species in a non-chemistry model.
Expanded polyQ aggregates interact with Sarco-endoplasmic reticulum calcium ATPase and Drosophila Inhibitor of Apoptosis Protein1 to regulate polyQ mediated neurodegeneration in Drosophila
Authors: Maurya Chandan Kumar , TAPADIA MADHU G 
Published: February 2023

Abstract
Polyglutamine (polyQ) induced neurodegeneration is one of the leading causes of progressive neurodegenerative disorders characterized clinically by deteriorating movement defects, psychiatric disability, and dementia. Calcium [Ca2+] homeostasis, which is essential for the functioning of neuronal cells, is disrupted under these pathological conditions. In this paper, we simulated Huntington's disease phenotype in the neuronal cells of the Drosophila eye and identified [Ca2+] pump, Sarco-endoplasmic reticulum calcium ATPase (SERCA), as one of the genetic modifiers of the neurodegenerative phenotype. In this paper, we show genetic and molecular interaction between polyglutamine (polyQ) aggregates, SERCA and DIAP1. We present evidence to show that polyQ aggregates interact with SERCA and alter its dynamics, leading to enhanced ER calcium and toxicity. Downregulating SERCA lowers the enhanced calcium levels in the ER and rescues, morphological and functional defects caused due to expanded polyQ repeats. Cell proliferation markers such as Yorkie (Yki), Scalloped (Sd), and phosphatidylinositol 3 kinases/protein kinase B (PI3K/Akt), also respond to varying levels of calcium due to genetic manipulations, adding to the amelioration of degeneration. These results imply that neurodegeneration due to expanded polyQ repeats is sensitive to SERCA activity, and its manipulation can be an important step toward its therapeutic measures.
Enhanced trace amount terahertz fingerprint spectroscopy using symmetrical spoof surface plasmon metasurfaces
Authors: YANG JI , LI XIANGJUN , yan dexian , ZHANG LE , ZHAO YANG , LIU HUADONG 
Published: February 2023

Abstract
Since the frequency range accords with the fingerprint characteristics of many biomacromolecular materials, terahertz (THz) waves are widely used in the detection of biomacromolecular materials. However, when it is used for real application, how to measure the spectral of trace-amount analysis samples is a major challenge. By localizing electromagnetic fields in dielectric meta-grating or metasurfaces is the most popular way to enhance the spectral signal. In this paper, we propose a design of THz structure that excites a series of spoof surface plasmon (SSP) sharp resonances on the geometry multiplexing metals metasurfaces in one layer to enhance the terahertz absorption spectrum of biomacromolecular materials. The structure can be manufactured and measured easily. The enhanced absorption spectrum can be built by linking a series of SSP sharp resonances. Numerical results show that the absorption enhancement factor about 160 times for the 0.2µm lactose film sample in the frequency range of 0.45–0.61 THz. This design will provide a better method for the detection of biomacromolecular materials.
Topical bee venom nanoemulsion in collagen-induced arthritis model: effects on biochemical and hematological parameters
Authors: Yousefpoor Yaser , Amani Amir , Hashemi Seyed Mohammad , Afgar Ali , Amini Seyed Mohammad , Mirzaei-Parsa Mohamad Javad 
Published: February 2023

Abstract
Background: Traditionally, Bee venom (BV) is used through stinging or injection to treat rheumatoid arthritis (RA). This study aimed to assess the side effects of bee venom nanoemulsions (BV-NEs) in the collagen-induced arthritis (CIA) model by examining biochemical and hematological parameters.
Methods: The BV-NEs were prepared, and the CIA model was induced in rats. After the seventh day, the groups were topically treated for two weeks as the following: blank (free treatment), negative control (NE-0), positive control (hydrocortisone acetate ointment 1%, 50 mg/day), BV control (37.5 µg/ml/day), and BV-NEs receiving 75, 37.5, 18.75, and 9.37 µg/ml/day. Three steps of blood sampling were done on days 0, 7, and 21 (healthy rats, before and at the end of treatment, respectively).
Results: The results revealed that blood levels of glucose, cholesterol, urea, aspartate aminotransferase (AST), alkaline phosphatase (ALP), white blood cell (WBC), and %neutrophil significantly increased before the treatment. Nevertheless, most parameters declined at the end of the treatment compared to the blank and negative control groups about BV-NEs dose-dependently. The drastic changes in biochemical parameters in the CIA model indicated the effect of the immune system function on the metabolic system. Also, NE's impact of BV passed through the skin on these items.
Conclusions: BV-NEs can reduce inflammation caused by arthritis without acute adverse effects on the routine biochemical and hematological parameters.
Heparan sulfate-dependent transport of CCL2 across an in vitro model of the human blood-brain barrier
Authors: Williams Lindsey M. , Fujimoto Takashi , Weaver Riley R. , Reed May J. , Erickson Michelle A. 
Published: February 2023

Abstract
Background: Transport of immune-active substances across the blood-brain barrier (BBB) is an important mechanism of neuroimmune regulation. CCL2 is among the chemokines known to cross the intact BBB in the blood-to-brain direction and is supported to do so in mice through interactions with heparan sulfate (HS)-containing components of the endothelial glycocalyx. The goal of this study was to characterize blood-to-brain transport mechanisms of human CCL2 in a human induced pluripotent stem-cell (iPSC)- derived in vitro model of the BBB.
Methods: Human brain endothelial-like cells (iBECs) were differentiated using established methods and then changed to heparin-free medium. All experiments were conducted 9 days after seeding differentiated iBECs on permeable culture inserts or tissue culture plates. Human recombinant CCL2 and bovine serum albumin (Alb) as a leakage tracer was labeled with 125I and 131I, respectively, and their flux across the monolayer was quantified by calculating the permeability-surface area coefficient. Transport of 125I-CCL2 and 131I-Alb was evaluated at baseline, in the presence of a CCR2 inhibitor and heparin, following treatment with heparinases, and following treatment with the heparan sulfate synthesis inhibitor GalNaz to evaluate HS-dependent mechanisms of transport. We further determined the mechanism of 125I-CCL2 transcytosis using inhibitors of clathrin, caveolae, and dynamin.
Results: We found that iBECs have a functional blood-to-brain transport system for CCL2. Similar to our previous findings in mice, heparin inhibited CCL2 transport whereas the CCR2 inhibitor did not. We further showed that both heparinase treatment and treatment with GalNaz inhibited CCL2 transport across the BBB, supporting the requirement for HS in CCL2 transport. CCL2 transcytosis was clathrin-independent and caveolae and dynamin-dependent.
Conclusions: Our findings support that human CCL2 is transported across the human BBB in vitro by a mechanism that was HS-dependent, caveolae and dynamin-dependent, and clathrin-independent. Our findings underscore the utility of iBECs for the study of mechanisms of heparan sulfate/glycocalyx interactions in the transport of substances across the BBB.
Healthcare resource utilization of patients with mitochondrial disease in an outpatient hospital setting
Authors: Haque Sameen , Crawley Karen , Shrestha Rupendra , Schofield Deborah , Sue Carolyn 
Published: February 2023

Abstract
Background and Objectives:
Mitochondrial diseases present as multi-system disorders requiring a comprehensive multidisciplinary approach. The data on healthcare resource utilization associated with mitochondrial diseases and the clinical drivers of these costs is limited including for the out-patient setting where the majority of the clinical care for mitochondrial patients occurs. We performed a cross-sectional retrospective study of out-patient healthcare resource utilization and costs for patients with a confirmed diagnosis of mitochondrial disease.
Methods:
We recruited participants from the Adult Mitochondrial Disease Clinic in Sydney and stratified them into three groups: those with mitochondrial DNA (mtDNA) mutations (Group 1), those with nuclear DNA (nDNA) mutations and the predominant phenotype of chronic progressive external ophthalmoplegia (CPEO) or optic atrophy(Group 2) and those without a confirmed genetic diagnosis but clinical criteria and muscle biopsy findings supportive of a diagnosis of mitochondrial disease (Group 3). Data was collected through retrospective chart review and out-patient costs were calculated using the Medicare Benefits Schedule.
Results:
We analyzed the data from 91 participants and found that Group 1 had the greatest average out-patient costs per person per annum ($838.02; SD 809.72). Neurological investigations were the largest driver of outpatient healthcare costs in all groups (average costs per person per annum: - Group 1: $364.11; SD 340.93, Group 2: $247.83; SD 113.86 and Group 3: $239.57; SD 145.69) consistent with the high frequency (94.5%) of neurological symptoms. Gastroenterological and cardiac-related out-patient costs were also major contributors to out-patient healthcare resource utilization in Groups 1 and 3. In Group 2, ophthalmology was the second-most resource intensive specialty ($136.85; SD 173.35). The Group 3 had the greatest average healthcare resource utilization per person over the entire duration of out-patient clinic care ($5815.86; SD 3520.40) most likely due to the lack of a molecular diagnosis and a more customized management approach.
Conclusion:
The drivers of healthcare resource utilization are dependent on the phenotype-genotype characteristics and neurological, cardiac, and gastroenterological costs are the top three drivers in the out-patient clinics unless the patient has nDNA mutations with predominant phenotype of CPEO and/or optic atrophy wherein ophthalmological-related costs were the second most resource intensive driver.
Experimental Investigation on the Correlation between Total Pressure and Swirl Distortions
Authors: Zhang Haideng , Sun Enbo 
Published: February 2023

Abstract
To lay the foundations of establishing low-cost methods which can predict characteristics of swirl distortion inexpensively, experimental investigations on the correlation between total pressure and swirl distortions of a typical S-duct intake model are implemented. Baffle plate is installed at the inlet of the intake model to generate strong inlet flow distortions, and the flow field at the outlet of the duct is measured using five-hole probes, which are calibrated using zonal calibration algorithm. Total pressure and swirl distortions associated with the baffle plate installed at the upper part of the intake model show no correlations. When baffle plate is installed at left/right part of the intake model, strong total pressure and swirl distortions will interact with each other, and both strength and circumferential location of the two types of distortion are well correlated by linear relationships. When baffle plate is installed at the lower part of the intake model, strong total pressure and paired swirl distortions, whose strength are well correlated by linear relationship, are induced.