bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical application of modified hip joint lateral position in femoral neck fracture
Authors: Liu Haitian , Gao Enpeng , Cao Lei , Zheng Yongli , Wen JinXu , Kong Lingde , Wu Wenjuan , Zhang Zuzhuo , Zhong Zhiwei 
Published: May 2023

Abstract
Background
To show the femoral neck better in hip lateral view of X-ray, we design a modified hip lateral view, and then investigate the value in femoral neck fractures.
Methods
CT images of 10 normal hip joints for 3D reconstruction were selected, the Mimics Medical 21.0 were used, and rotating the proximal femur was to find the most suitable angle for showing the femoral neck well, designed the modified lateral view according to this angle. We collected 25 femoral neck fracture and 25 healthy cases for observation and control group. And two groups were all taken hip anteroposterior, cross-table lateral and modified lateral view, which were analyzed by two radiologists to score the anatomical structures of the articular surface, femoral head, subfemoral head region, femoral neck, basal region and intertrochanteric region. Friedman Test was used to analyze the score of femoral neck at different angles. T test and Wilcoxon signed ranks test were to compare inter-groups.
Results
The modified lateral view was designed as follows: the subjects were supine, with the sagittal axis biased toward the healthy side at an angle of approximately 20° to the long axis of the examination table, the hip joint flexed at 45°, the lower extremity abducted at 40°, the centerline inclined 45° toward the head, and the centerline aligned with the center of the groin. The modified lateral view showed the femoral head, subfemoral head region and femoral neck more clearly than the lateral cross-table view, but the cross-table lateral showed the femoral neck basal and intertochanteric region better. In addition, the time of taking the modified lateral view was significantly less than the cross-table lateral view (healthy group: 0.752min ± 0.161 vs 0.596min ± 0.151, P < 0.001; fracture group: 1.128min ± 0.404 vs 0.924min ± 0.395, P < 0.001).
Conclusions
The modified lateral view can obtain a standard sagittal image of femoral neck, which can show the dislocation and angulation of the sagittal femoral neck fracture clearly, and improve the accuracy of diagnosis. And it is more convenient and easier for patients to cooperate, which is worthy promoting and applying in clinical work.
Observation of photonic Peierls transition for manipulating electromagnetic waves in metallic periodic structures
Authors: Wu Chia Ho , Liu Chengyang , Lin Xianqing , Wang Wei , Guo Yi Chun , Wang Zhuoyua , Ye Guoqiang , He Fang , Ni Donghua , Wang Xiaolong , Shen Linfang , Shen Jianqi 
Published: May 2023

Abstract
Peierls transition that modifies electronic band structure has attracted intensive attention in solid state physics. In the present work, a photonic analog of Peierls transition has been observed in a 1-D triangular metal diaphragm array, where the photonic bandgap structures have been designed at will by adjusting periodically the metal diaphragm positions. It is shown by the numerical analysis that the transmission and radiation effect of the present periodic metal structure designed through the Peierls transition rule exhibits the behavior significantly different from the original periodic structure with each unit cell containing a metal diaphragm. The near- and far-field measurement results are in good agreement with our theoretical simulation. The present effect of photonic Peierls transition can serve as a working mechanism for designing new types of guided wave devices. It can be seen that the photonic Peierls transition would be one of the simplest ways for modifying the transport characteristics of periodic structures.
Visual Attention and Memory in Professional Traders: A Behavioural Study
Authors: Bossi Francesco , Malizia Andrea P. , D’Arcangelo Sonia , Maggi Francesca , Lattanzi Nicola , Ricciardi Emiliano 
Published: May 2023

Abstract
Professional traders need to process a large amount of visual information in their daily activity to judge how risky it is to trade specific investment products. Despite some studies investigating the effects of display clutter on traders, visual attention and memory were never investigated in controlled experimental tasks in this population. Following a preliminary study with 30 participants, visual selective attention and visual working memory were measured and compared between two groups of 15 traders and 15 non-traders (salespeople, acting as a control group) from a large-scale banking group in three experimental tasks measuring selective attention in complex visual contexts, simulating display clutter situations (Visual search), cognitive interference (Stroop task), and a delayed recall visual working memory task. In the Visual search task, traders displayed a better Inverse Efficiency Score (IES) than non-traders for small display sets, while their performance overlapped for large sets. In the Stroop task, traders showed better IES than non-traders but were nevertheless affected by cognitive interference. The memory task highlighted no significant differences between the groups. Therefore, this study found an advantage in traders’ attention when processing visual information in small sets with no retention. This result could influence trading activity - determining an immediate use of relevant visual information in decision making - and traders’ display layout organization.
Effects of micro-algae oil replacement on growth, omega-3 deposition and gut microbiome composition of Nile tilapia (Oreochromis niloticus)
Authors: Trevi S. , Webster T. M. Uren , Consuegra S. , de Leaniz C. Garcia 
Published: May 2023

Abstract
Microalgae offer a sustainable source of omega-3 fatty acids that can replace fish oil in aquafeeds, but the nutritional benefits are not always clear, particularly when microalgae are used as complete oil replacements in starter feeds. We compared the survival, growth, omega-3 deposition and composition of the gut microbiota of Nile tilapia fed with aquafeeds that differed in dietary oil, from plant, fish and microalgae (Schizochytrium) origins. Survival was not affected by diet, but fish fed a diet where the entire oil component (5%) was replaced by microalgae oil grew twice as fast than fish fed plant oil or a mixture of plant and fish oil. Dietary omega-3 content was strongly correlated with omega-3 deposition in the fish fillet. Complete replacement of fish oil by plant oil caused a significant increase in the abundance of Aeromonadaceae which is often associated with an inflammatory response in the fish gut. In contrast, when fish oil was replaced by microalgae oil an increase in Peptostreptococcaceae and Mycobacteriaceae was observed. Our study indicates that Schizochytrium oil can be used to improve the growth of Nile tilapia and increase its omega-3 content without any of the detrimental effects on the gut microbiome typically associated with some plant oil replacements.
Impact of Elobixibat on Liver Tumors, Microbiome and Bile Acid Levels in a Mouse Model of Nonalcoholic Steatohepatitis
Authors: Sugiyama Yoshiaki , Yamamoto Kenta , Honda Takashi , Kato Asuka , Muto Hisanori , Yokoyama Shinya , Ito Takanori , Imai Norihiro , Ishizu Yoji , Nakamura Masanao , Asano Tomomi , Zaitsu Kei , Ishigami Masatoshi , Fujishiro Mitsuhiro , Kawashima Hiroki 
Published: May 2023

Abstract
Background
Elevated bile acid levels have been associated with liver tumors in fatty liver. Ileal bile acid transporter inhibitors may inhibit bile acid absorption in the distal ileum and increase bile acid levels in the colon, potentially decreasing serum and hepatic bile acids. This study investigated the impact of these factors on tumors.
Methods
C57BL/6J mice received a one-time intraperitoneal injection of 25 mg/kg of diethylnitrosamine. They were fed a choline-deficient high-fat diet for 20 weeks starting from 8 weeks of age, with or without elobixibat (EA Pharma, Tokyo, Japan).
Results
Both groups showed liver fat accumulation and fibrosis, with no significant differences between the two groups. However, mice treated with elobixibat showed fewer liver tumors. The total serum bile acid levels, including free-/tauro-/gly- conjugated bile acids and tauro-α/β-muricholic acids in the liver, were noticeably reduced with elobixibat. The proportion of gram-positive bacteria in feces was significantly lower in the group treated with elobixibat (5.4%) than in the group without elobixibat (33.7%).
Conclusion
Elobixibat suppressed tumor growth by inhibiting bile acid reabsorption and decreasing total bile acid and primary bile acid levels in the serum and liver. Additionally, the presence of bile acids in the colon may have led to a significant reduction in the proportion of gram-positive bacteria, potentially resulting in decreased secondary bile acid synthesis.
A cell-free strategy for profiling of intracellular antibiotic sensitivity and resistance
Authors: Chengan Kameshwari , Hind Charlotte , Nagappa Lakshmeesha , Wand Matthew , Sutton John , Hanson Tanith , Smales Mark , Tsaousis Ruben , Escolano Ruben Martin , Bengoechea Jose , Moore Simon 
Published: May 2023

Abstract
Antimicrobial resistance (AMR) is a pandemic spread across multiple priority infectious disease threats. Herein, we present a Klebsiella pneumoniae cell-free gene expression (CFE) system for the rapid profiling of intracellular antibiotic sensitivity and resistance. Specifically, this cell-free approach provides multiple antibiotic targets, which we exploit to identify which antibiotics are potent inside the cell but have limited transport. We also derive CFE systems from a selection of laboratory-adapted K. pneumoniae AMR variants, and as an exemplary case, show that a single RNA polymerase beta subunit variant H526L confers a 58-fold increase in CFE resistance to rifampicin. Overall, our cell-free model provides a safe (i.e., non-living) and rapid platform suitable for studying AMR variants in a Containment Level 1 laboratory. The approach is generalisable to laboratory and clinical strains and provides a new experimental tool that will significantly advance the understanding of antibiotic transport, AMR and provides a new infectious disease model.
SEMORE: SEgmentation and MORphological fingErprinting by machine learning automates super-resolution data analysis
Authors: Bender Steen , Dreisler Marcus , Zhang Min , Kaestel-Hansen Jacob , Hatzakis Nikos 
Published: May 2023

Abstract
The morphology of protein assemblies impacts their behavior and contributes to beneficial and aberrant cellular responses. While single-molecule localization microscopy provides the required spatial resolution to investigate these assemblies, the lack of universal robust analytical tools to extract and quantify underlying structures limits this powerful technique. Here we present SEMORE, a semi-automatic machine learning framework for universal, system and input-dependent, analysis of super-resolution data. SEMORE implements a multi-layered density-based clustering module to dissect biological assemblies and a morphology fingerprinting module for quantification by multiple geometric and kinetics-based descriptors. We demonstrate SEMORE on simulations and diverse raw super-resolution data; time-resolved insulin aggregates and imaging of nuclear pore complexes. SEMORE extracts and quantifies all protein assemblies enabling classification of heterogeneous insulin aggregation pathways and NPC geometry in minutes. SEMORE is a general analysis platform for super-resolution data, and being the first time-aware framework can also support the rise of 4D super-resolution data.
Exploration of the Molecular Mechanism of Intercellular Communication in Paediatric Neuroblastoma by Single-Cell Sequencing
Authors: Chu Jing 
Published: May 2023

Abstract
Background
Neuroblastoma (NB) is an embryonic tumour originating from the sympathetic nervous system that most often occurs in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing research suggests that intercellular communication within the tumour microenvironment is closely related to tumour development.
Methods
This study aimed to construct a prognosis-related intercellular communication-associated genes (ICAGs) model by single-cell sequencing and transcriptome sequencing to predict the prognosis of patients with NB for precise management. Single-cell data from patients with NB were downloaded from the Gene Expression Omnibus database for comprehensive analysis. Moreover, prognosis-related genes were screened in the TARGET database based on epithelial cell marker genes through a combination of Cox regression and Lasso regression analyses, using GSE62564 and GSE85047 for external validation. Patients’ risk scores were calculated, followed by immune infiltration analysis, drug sensitivity analysis, and enrichment analysis of risk scores, which were conducted for the prognostic model.
Results
We used the feature selection algorithm with Lasso regression to screen for characteristic genes in NB and developed a 21-gene prognostic model. The risk scores were highly correlated with multiple immune cells and common anti-tumour drugs. In addition, the risk score was identified as an independent prognostic factor for NB.
Conclusions
In this study, we constructed and validated a prognostic signature based on epithelial marker genes, which may provide useful insights into the development and prognosis of NB.
RNF213 inhibits migration via mediating KRT16 ubiquitination in lung adenocarcinoma cell
Authors: Pei Xiaofeng , Liu Xiaoli , Zeng Huanwen , Zeng Fangfang , Wei Chunren , Zhang Zhaoyuan , Chen Nan , Zhang Hongyu 
Published: May 2023

Abstract
Background Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors, and lung adenocarcinoma (LUAD) accounts for 40% of NSCLC. Ring finger protein 213(RNF213) has been shown to inhibit the progression of many different cancers including glioblastoma and breast cancer. However, the role of RNF213 in LUAD has not been reported. The aim of this study was to investigate the effect of RNF213 on the progression of LUAD.Methods The expression of RNF213 in LUAD tissues was analyzed by western blot, TCGA (The Cancer Genome Atlas) and GTEx (Genotype_Tissue Expression Project) databases. Kaplan-Meier Plotter database was used to predict the clinical significance of RNF213 in LUAD. We determined the role of RNF213 in LUAD cell lines through CCK-8 assay, migration and invasion assay. The relationship of RNF213 and KRT16 were demonstrated via co-immunoprecipitation, ubiquitination, protein degradation assay and immunoblotting. We next confirmed the oncogenetic role of KRT16 using migration and rescue assay. The clinical roles of RNF213 and KRT16 were explored by immunohistochemical staining assay (IHC) and Kaplan-Meier survival analysis.Results Our data manifested that RNF213 expression was reduced in LUAD, thereby affecting the prognosis of LUAD. The molecular study revealed that RNF213 functioned by promoting KRT16 ubiquitination. IHC data analysis showed that KRT16 was negatively correlated with RNF213 protein expression, and downregulation of RNF213 was associated with poor overall survival.Conclusion RNF213 is a potential tumor suppressor that inhibits migration of LUAD cell by mediating KRT16 ubiquitination.
Validation of the Italian Version of the Peyronie’s Disease Questionnaire (PDQ)
Authors: Grazia Massimo Di , Ongaro Luca , Rizzo Michele , Cocci Andrea , Verze Paolo , Zucchi Alessandro , Cai Tommaso , Salame' Leonardo , Garaffa Giulio , Trombetta Carlo , Liguori Giovanni , Traunero Fabio 
Published: May 2023

Abstract
Background/objectives: Peyronie's disease (PD) is a connective tissue disorder characterized by the formation of fibrous plaques in the tunica albuginea of the penis, resulting in pain, deformity, and erectile dysfunction. The Peyronie's Disease Questionnaire (PDQ) assesses the severity of symptoms, including pain, penile curvature, and erectile dysfunction, as well as the impact on sexual function, emotional well-being, and overall quality of life. Previous studies validated the PDQ US version and confirmed its test–retest reliability and responsiveness. The aim is to translate and validate the Italian version of the PDQ to be used in clinical practice and in PD research studies in Italy.Methods A non-interventional, observational study with 80 PD patients was conducted in 6 Italian high-volume centers, completing the PDQ in two different study visits. Patients received no type of treatment.Results PDQ-I has excellent test-retest reliability in the Italian population (ICC were between 0.86 and 0.92). Moreover, PDQ-I provides strong internal consistency, with all three scale scores exceeding the objective Cronbach's alpha coefficient of ≥ 0.70Conclusions The translation and validation of the PDQ for the Italian population provides a valid, useful, and reliable tool to clinically evaluate the quality of life of PD patients and to improve studies on the subject.
Clinical importance of TYMS and ENOSF1 genetic variants and mRNA expression levels in response to chemotherapy in Iranian gastric cancer patients
Authors: Arjmandi Khadijeh , Salahshourifar Iman , Irani Shiva , Ameli Fereshteh , Esfandbod Mohsen 
Published: May 2023

Abstract
Background Genetic variants are powerful tools for predicting the effectiveness of chemotherapy drugs. Thymidylate synthase (TS) is a crucial enzyme in gastric cancer patients given neoadjuvant chemotherapy based on 5-Fluorouracil (5-FU). The enolase superfamily member 1 (ENSOF1) variant affects TS gene (TYMS) expression and thus may affect chemoresistance in gastric cancer. Hence, this study aims to examine the 28 bp VNTR variant on the 5'UTR of the TYMS gene and rs2612091 and rs2741171 variants of the ENOSF1 and the possible effects of their alleles on chemo-resistance, survival, and gene expression among gastric cancer cases.Methods and Results Genetic analysis was performed on 100 matched FFPE blocks for normal tissues of gastric cancer cases. All patients were treated with neoadjuvant chemotherapy based on 5-FU. RNA was obtained from the tumor and matched normal tissues. The 28 bp VNTR, rs2612091, and 2741171 variants were genotyped, and the gene expression was analyzed using Real-Time PCR. A significant association was observed between genotypes of VNTR and the treatment (p = 0.032). Cases with the 2R3R genotype had a better response to the treatment. The highest and lowest average survival times of patients were observed in the 3R3R and 2R2R genotypes, respectively (p = 0.003). The 3R3R genotype was associated with higher TYMS expression (P < 0.001). There was a significant relationship between genotypes rs2612091 and the treatment (p = 0.017).Conclusions This research indicated that genotyping and gene expression analysis of TYMS and ENOSF1 might be used to predict gastric cancer survival and responsiveness to 5-FU-based neoadjuvant treatment.
Facilitators and barriers to communication in rehabilitation services across healthcare levels: A qualitative case study in a Norwegian context
Authors: Skumsnes Randi , Thygesen Hilde , Groven Karen Synne 
Published: May 2023

Abstract
Background People experiencing functional decline following injury or illness often need multiple and combined interventions in their rehabilitation processes. This means that collaboration and coordination between the involved healthcare professionals are essential. Interprofessional collaboration has, to a large extent, been explored in previous research, which has indicated the importance of facilitators of communication. Despite efforts in both research and policy, challenges in practice continue, both in the collaboration across specialist and primary levels of healthcare and within primary healthcare. Context-bound research is needed to gain insight into the complexity of these communication challenges. In this study we explore facilitators and barriers to communication in the context of neurological rehabilitation from specialist care at hospital to municipality primary care in a region of Norway. We address the transfer and exchange of relevant patient information between involved healthcare professionals.Methods A qualitative case study design was used to explore the exchange of patient information in the neurological rehabilitation processes for four patients. The data collection included participant observations in communication situations and an exploration of the electronic patient records of the same four patients. Reflexive thematic analysis was used to code and analyse the data, and the theory of practice architecture was used to interpret and structure the findings.Results We found three main barriers to communication: lack of a common understanding and culture for rehabilitation, poor access to written information and unclear authority and responsibilities for sharing information. These barriers are intertwined and influence communication practices.Conclusion The chains of interventions in the rehabilitation process depend on extensive information exchange among many of the health professionals involved, making collaboration complex. The barriers to exchanging relevant patient information need to be acknowledged as well as how informal relations among the involved facilitate the flow of information. More extensive use of joint communication tools may contribute to handling the complexity and to reducing barriers to communication.