bluuebunny/crossref_metadata_2025_split huggingface dataset:
UBE2J1 inhibits colorectal cancer progression by promoting ubiquitination and degradation of RPS3
Authors: Wang Tuo , Jin Chi , Yang Peng , Chen Zhihao , Ji Jiangzhou , Sun Qingyang , Yang Sheng , Feng Yifei , Tang Junwei , Sun Yueming 
Published: December 2022

AbstractUbiquitin-conjugating enzyme E2 J1 (UBE2J1) has been proven to participate in the ubiquitination of multiple substrate proteins. However, the underlying mechanisms of UBE2J1 as a ubiquitin-conjugating enzyme participating in cancer development and progression remain largely unknown. Here, we identified that UBE2J1 is downregulated in colorectal cancer (CRC) tissues and cell lines which are mediated by DNA hypermethylation of its promoter, and decreased UBE2J1 is associated with poor prognosis. Functionally, UBE2J1 serving as a suppressor gene inhibits the proliferation and metastasis of CRC cells. Mechanistically, UBE2J1-TRIM25, forming an E2-E3 complex, physically interacts with and targets RPS3 for ubiquitination and degradation at the K214 residue. The downregulated RPS3 caused by UBE2J1 overexpression restrains NF-κB translocation into the nucleus and therefore inactivates the NF-κB signaling pathway. Our study revealed a novel role of UBE2J1-mediated RPS3 poly-ubiquitination and degradation in disrupting the NF-κB signaling pathway, which may serve as a novel and promising biomarker and therapeutic target for CRC.
The immunological role of ADAMs in the field of gastroenterological chronic inflammatory diseases and cancers: a review
Authors: Arai Jun , Otoyama Yumi , Nozawa Hisako , Kato Naoya , Yoshida Hitoshi 
Published: December 2022

AbstractMetalloproteinases cleave transmembrane proteins that play critical roles in inflammation and cancers. Metalloproteinases include a disintegrin and metalloprotease (ADAM), which we previously examined using a fluorescence assay system, and described their association with resistance to systemic therapy in cancer patients. There are also many reports on the relation between ADAM expression and the prognosis of patients with gastroenterological chronic inflammatory diseases and cancers. Inhibiting their immunomodulating activity in chronic inflammation restores innate immunity and potentially prevents the development of various cancers. Among the numerous critical immune system-related molecules, we focus on major histocompatibility complex class I polypeptide-related sequence A (MICA), MICB, intracellular adhesion molecule (ICAM)-1, TNF-α, IL-6 receptor (IL-6R), and Notch. This review summarizes our current understanding of the role of ADAMs in gastroenterological diseases with regard to the immune system. Several Food and Drug Administration (FDA)-approved inhibitors of ADAMs have been identified, and potential therapies for targeting ADAMs in the treatment of chronic inflammatory diseases and cancers are discussed. Some ongoing clinical trials for cancers targeting ADAMs are also introduced.
The neurobiology of apathy in depression and neurocognitive impairment in older adults: a review of epidemiological, clinical, neuropsychological and biological research
Authors: Steffens David C. , Fahed Mario , Manning Kevin J. , Wang Lihong 
Published: December 2022

AbstractApathy is a common condition that involves diminished initiative, diminished interest and diminished emotional expression or responsiveness. It is highly prevalent in the context of a variety of neuropsychiatric disorders and is related to poor health outcomes. Presence of apathy is associated with cognitive and functional decline in dementia. Despite its negative impact on health, there is no definitive treatment for apathy, a clinical reality that may be due in part to lack of knowledge about assessment, neuropsychological features and neurobiological underpinnings. Here, we review and synthesize evidence from clinical, epidemiological, neuropsychological, peripheral biomarker and neuroimaging research. Apathy is a common feature of depression and cognitive disorders and is associated with impairment in executive function. Neuropsychological and neuroimaging studies point to dysfunction of brain circuitry involving the prefrontal cortex, especially the dorsolateral prefrontal cortex circuit, the dorsomedial prefrontal cortex circuit, and the ventromedial prefrontal cortex circuit. However, inconsistent findings, particularly in neuroimaging may be due to heterogeneity of apathy symptoms (with a need to better elucidate subtypes), neuropsychiatric comorbidities, the severity of cognitive impairment and other factors. These factors need to be accounted for in future studies so that biomarker research can make progress. On the whole, the literature on apathy has identified likely neurocognitive, peripheral biomarker and neuroimaging targets for understanding apathy, but also points to the need to address methodological issues that will better inform future studies. In turn, as we learn more about the underpinning of apathy and its subtypes, subsequent research can focus on new neurally based interventions that will strengthen the clinical management of apathy in the context of its comorbidities.
3D collagen migration patterns reveal a SMAD3-dependent and TGF-β1-independent mechanism of recruitment for tumour-associated fibroblasts in lung adenocarcinoma
Authors: Juste-Lanas Yago , Díaz-Valdivia Natalia , Llorente Alejandro , Ikemori Rafael , Bernardo Alejandro , Arshakyan Marselina , Borau Carlos , Ramírez Josep , Ruffinelli José Carlos , Nadal Ernest , Reguart Noemí , García-Aznar José M. , Alcaraz Jordi 
Published: December 2022

Abstract
Background
The TGF-β1 transcription factor SMAD3 is epigenetically repressed in tumour-associated fibroblasts (TAFs) from lung squamous cell carcinoma (SCC) but not adenocarcinoma (ADC) patients, which elicits a compensatory increase in SMAD2 that renders SCC-TAFs less fibrotic. Here we examined the effects of altered SMAD2/3 in fibroblast migration and its impact on the desmoplastic stroma formation in lung cancer.

Methods
We used a microfluidic device to examine descriptors of early protrusions and subsequent migration in 3D collagen gels upon knocking down SMAD2 or SMAD3 by shRNA in control fibroblasts and TAFs.

Results
High SMAD3 conditions as in shSMAD2 fibroblasts and ADC-TAFs exhibited a migratory advantage in terms of protrusions (fewer and longer) and migration (faster and more directional) selectively without TGF-β1 along with Erk1/2 hyperactivation. This enhanced migration was abrogated by TGF-β1 as well as low glucose medium and the MEK inhibitor Trametinib. In contrast, high SMAD2 fibroblasts were poorly responsive to TGF-β1, high glucose and Trametinib, exhibiting impaired migration in all conditions.

Conclusions
The basal migration advantage of high SMAD3 fibroblasts provides a straightforward mechanism underlying the larger accumulation of TAFs previously reported in ADC compared to SCC. Moreover, our results encourage using MEK inhibitors in ADC-TAFs but not SCC-TAFs.
Nanowire-assisted freestanding liquid metal thin-film patterns for highly stretchable electrodes on 3D surfaces
Authors: Kim Minwoo , Cho Chulmin , Shin Wooseop , Park Jung Jae , Kim Jaewon , Won Phillip , Majidi Carmel , Ko Seung Hwan 
Published: December 2022

AbstractStretchable electronics is playing an integral role in fields such as wearable electronics and soft robots. Among soft conductive materials, liquid metal is drawing intense attention as an electrode material due to its liquid nature at room temperature. However, the merits of liquid metal conductor are limited by the presence of substrates or enclosed microchannels from physical disturbances by the underlying substrate when applying it to 3D surface and modifying complex circuit. To overcome this limitation, we develop freestanding patterned liquid metal thin-film conductor (FS-GaIn). FS-GaIn was achieved by introducing metal nanowires to liquid metal and subsequent sequential selective laser processing and etching of directly patterned traces. FS-GaIn can be applied directly to nonflat surface without substrates. When incorporated into electrical circuits, FS-GaIn shows high electrical conductivity, stretchability, and stability. The concept of freestanding liquid metal can open a functionality to the conventional liquid metal electronics.
Experiencing a slow passage of time was an indicator of social and temporal disorientation during the Covid-19 pandemic
Authors: Fernandez Velasco Pablo , Perroy Bastien , Gurchani Umer , Casati Roberto 
Published: December 2022

AbstractTime dilation was experienced in most countries and across the several years of the Covid-19 crisis: the passage of time was deemed slower than before the pandemic, and the distance to the beginning of the pandemic seemed longer than it really was. An outstanding question is how these two aspects of time judgements relate to other temporal, social and affective disturbances. We developed and validated a 59-item questionnaire to explore these questions. 3306 participants completed the questionnaire in France in May and June 2021. Here, we analyse group differences and find that both slow passage of time and long distance judgements were associated with larger disturbances across all domains under study. These included temporal disruptions—the aptness to project oneself into the future, the sense of a rift between pre-pandemic and pandemic time, the ability to locate oneself in time, the capacity to recall the order of past events—, as well as an overall sense of social disorientation, and trauma-specific disturbances. In contrast, both fast passage of time and short distance judgements were associated with beneficial effects across all of the mentioned domains. Our results indicate that perceived passage of time and temporal distance judgements are key indicators of social and temporal disorientation.
C-type lectin-like receptor (CLEC)-2, the ligand of podoplanin, induces morphological changes in podocytes
Authors: Tanaka Keiko , Tanaka Masafumi , Watanabe Nobuo , Ito Masatoshi , Pastan Ira , Koizumi Masahiro , Matsusaka Taiji 
Published: December 2022

AbstractPodoplanin (PDPN) is intensely expressed on the podocyte membrane in an evolutionally conserved manner. CLEC-2, the endogenous ligand of PDPN, is highly expressed in platelets and also exists in a soluble form in plasma. Normally, podocytes are sequestered from CLEC-2, but when the glomerular barrier is injured, podocytes gain access to CLEC-2. We tested the effects of CLEC-2 in podocytes in vitro and in vivo. Cultured podocytes treated with Fc-CLEC-2 demonstrated that CLEC-2 induced the dephosphorylation of ezrin, radixin, and moesin (ERM) proteins. Podocytes treated with Fc-CLEC-2 also showed the dissociation of F-actin filaments from PDPN, F-actin degradation, detachment, and round morphology. Next, we perfused normal mouse kidney in vivo with FLAG-CLEC-2. CLEC-2 induced dephosphorylation of ERM and widening of the foot processes of podocytes. Platelets were detected by immunostaining for CD41 in the urine of mice with podocyte injury, indicating that podocytes can encounter platelets when glomeruli are injured. Collectively, these observations suggest that when platelets leak through the injured glomeruli, CLEC-2 from the platelets acts on PDPN in podocytes and induces morphological change and detachment, which may further aggravate podocyte injury. Thus, PDPN on podocytes may work as a leaked-platelet sensor.
Development and validation of a predictive scoring system for in-hospital mortality in COVID-19 Egyptian patients: a retrospective study
Authors: Elgohary Mohamed AbdelSalam , Ali Asmaa , El-Masry Thanaa A. , Faidah Hani , Bantun Farkad , Elkholy Ahmad M. , Fahim Jaklin S. , Elgamal Nabila N. , Mohamed Mohamed Emam , Seadawy Mohamed G. , Helal Amro M. , De Waard Michel , Shishtawy Hesham M. , El-Bouseary Maisra M. 
Published: December 2022

AbstractSARS-CoV-2 virus has rapidly spread worldwide since December 2019, causing COVID-19 disease. In-hospital mortality is a common indicator for evaluating treatment outcomes. Therefore, the developing and validating a simple score system from observational data could assist in modulating the management procedures. A retrospective cohort study included all data records of patients with positive PCR for SARS-CoV-2. The factors that associated with mortality were analyzed, then allocation of potential predictors of mortality was executed using different logistic regression modeling, subsequently scoring system was developed from the most weighted predictors. The mortality rate of patients with COVID-19 pneumonia was 28.5% and 28.74%, respectively. The most significant factors that affected in-hospital mortality were old age (> 60 years), delay in hospital admission (> 4 days), high neutrophil/lymphocyte ratio “NLR” (> 3); higher computed tomography severity score; and CT-SS (> 20), in addition to using remdesivir and tocilizumab in the treatment protocol (P < 0.001 for all). The validity of the newly performed score was significant; the AUC was 85%, P < 0.001, and its prognostic utility was good; the AUC was 75%, P < 0.001. The prognostic utility of newly developed score system (EGY.Score) was excellent and could be used to adjust the treatment strategy of highly at-risk patients with COVID-19 pneumonia.
Multi-objective learning and explanation for stroke risk assessment in Shanxi province
Authors: Ma Jing , Sun Yiyang , Liu Junjie , Huang Huaxiong , Zhou Xiaoshuang , Xu Shixin 
Published: December 2022

AbstractStroke is the leading cause of death in China (Zhou et al. in The Lancet, 2019). A dataset from Shanxi Province is analyzed to predict the risk of patients at four states (low/medium/high/attack) and to estimate transition probabilities between various states via a SHAP DeepExplainer. To handle the issues related to an imbalanced sample set, the quadratic interactive deep model (QIDeep) was first proposed by flexible selection and appending of quadratic interactive features. The experimental results showed that the QIDeep model with 3 interactive features achieved the state-of-the-art accuracy 83.33%(95% CI (83.14%; 83.52%)). Blood pressure, physical inactivity, smoking, weight, and total cholesterol are the top five most important features. For the sake of high recall in the attack state, stroke occurrence prediction is considered an auxiliary objective in multi-objective learning. The prediction accuracy was improved, while the recall of the attack state was increased by 17.79% (to 82.06%) compared to QIDeep (from 71.49%) with the same features. The prediction model and analysis tool in this paper provided not only a prediction method but also an attribution explanation of the risk states and transition direction of each patient, a valuable tool for doctors to analyze and diagnose the disease.
Prevalence and characteristics of antibiotic prescription for acute COVID-19 patients in Japan
Authors: Hamada Seiji , Tokuda Yasuharu , Honda Hitoshi , Watari Takashi , Suzuki Tomoharu , Moromizato Takuhiro , Narita Masashi , Taniguchi Kiyosu , Shibuya Kenji 
Published: December 2022

AbstractCOVID-19 is a viral infection and does not require antibiotics. The study aimed to elucidate a prescribing pattern of antibiotics for COVID-19. A nationwide cross-sectional study was conducted in Japan. The Diagnosis and Procedure Combinations (DPC) data was used to collect information, covering 25% of all acute care hospitals in the country. In 140,439 COVID-19 patients, 18,550 (13.21%) patients received antibiotics. Antibiotics were prescribed more often in inpatients (10,809 out of 66,912, 16.15%) than outpatients (7741 out of 73,527, 10.53%) (p < 0.001). Outpatient prescription was significantly associated with older patients (odds ratio [OR], 4.66; 95% confidence interval [CI] 4.41–4.93) and a greater Charlson index (OR with one-point index increase, 1.22; 95% CI 1.21–1.23). Inpatient prescription was significantly associated with older patients (OR 2.10; 95% CI 2.01–2.21), male gender (OR 1.12, 95% CI 1.07–1.18), a greater Charlson index (OR with one-point increase, 1.06; 95% CI 1.05–1.07), requirement of oxygen therapy (OR 3.44; 95% CI 3.28–3.60) and mechanical ventilation (OR 15.09; 95% CI 13.60–16.74). The most frequently prescribed antibiotic among outpatients was cefazolin, while that among inpatients was ceftriaxone. Antibiotic prescription is relatively low for acute COVID-19 in Japan. Antibiotic prescription was associated with older age, multi-morbidity, severe disease, and winter season.
A bile acid-related prognostic signature in hepatocellular carcinoma
Authors: Zhang Wang , Zhang Yue , Wan Yipeng , Liu Qi , Zhu Xuan 
Published: December 2022

AbstractDue to the high mortality of hepatocellular carcinoma (HCC), its prognostic models are urgently needed. Bile acid (BA) metabolic disturbance participates in hepatocarcinogenesis. We aim to develop a BA-related gene signature for HCC patients. Research data of HCC were obtained from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) online databases. After least absolute shrinkage and selection operator (LASSO) regression analysis, we developed a BA-related prognostic signature in TCGA cohort based on differentially expressed prognostic BA-related genes. Then, the predictive performance of the signature was evaluated and verified in TCGA and ICGC cohort respectively. We obtained the risk score of each HCC patient according to the model. The differences of immune status and drug sensitivity were compared in patients that were stratified based on risk score. The protein and mRNA levels of the modeling genes were validated in the Human Protein Atlas database and our cell lines, respectively. In TCGA cohort, we selected 4 BA-related genes to construct the first BA-related prognostic signature. The risk signature exhibited good discrimination and predictive ability, which was verified in ICGC cohort. Patients were classified into high- and low-risk groups according to their median scores. The occurrence of death increased with increasing risk score. Low-risk patients owned favorable overall survival. High-risk patients possessed high immune checkpoint expression and low IC50 values for sorafenib, cisplatin and doxorubicin. Real-time quantitative PCR and immunohistochemical results validate expression of modeling genes in the signature. We constructed the first BA-related gene signature, which might help to identify HCC patients with poor prognosis and guide individualized treatment.
The impact of climate change, population growth and development on sustainable water security in Bangladesh to 2100
Authors: Kirby Mac , Mainuddin Mohammed 
Published: December 2022

AbstractThere are concerns that groundwater use for irrigation and for urban water supply is unsustainable in some parts of Bangladesh, particularly in the agriculturally productive northwest region. We use an integrated population – GDP – food – water model to examine water demand to 2100 in Bangladesh in development scenarios relevant to food and water security. The results indicate that irrigation water demand is projected to increase in coming decades, but later in the century it may decrease due to increasing crop yields and a falling population. The increased demand is greatest in the northwest region and, if unchecked, would increase concerns there about the unsustainable use of groundwater. The growth in demand is determined particularly by growth in crop yields, population growth and the fraction of food demand satisfied by imports. An extreme hot-dry climate change scenario has a lesser impact. This suggests that, in principle, Bangladesh can offset the impacts of climate change on irrigation water demand through its domestic policies. Urban water use currently also leads to concerns over unsustainable groundwater use. Our results suggest that urban water demand is likely to grow proportionately significantly more than irrigation water demand. Alternative sources for urban water are therefore urgently required.
Electroactive CTAB/PVDF composite film based photo-rechargeable hybrid power cell for clean energy generation and storage
Authors: Molla Sanoar , Khatun Farha , Rajak Ujjwal , Bagchi Biswajoy , Das Sukhen , Thakur Pradip 
Published: December 2022

AbstractHerein, electroactive polymer based photo-induced hybrid power cell has been developed using CTAB/PVDF composite film in a sustainable manner. First high dielectric polymer film has been prepared by doping CTAB in PVDF matrix via solution casting method. In the basic configuration of this hybrid power cell, aqueous electrolyte solution of PVA-MnO2-Eosin Y has been utilized as solar light absorber and photo-electron generator whereas the high dielectric CTAB/PVDF (~ 400) is used as dielectric separator cum storage part in a very transparent way. The cell shows maximum voltage $$\sim$$
∼
1.1 V with short-circuit current density ~ 7.83 mA/cm2 under ~ 110 mW/cm2 normal light illumination. The device reveal almost same performance for a long time (30 days). The high storage impact of the hybrid cell is investigated by its promising conversion efficiency $$\sim 4.48 \%$$

∼
4.48
%

with energy density and power density $$\sim 26.9$$

∼
26.9

mWh/m2 and $$\sim$$
∼
5.5 W/m2 respectively.
The role of 3D speckle tracking echocardiography in the diagnosis of obstructive sleep apnea and its severity
Authors: Chetan Ioana Maria , Gergely-Domokos Bianca , Beyer Ruxandra , Tomoaia Raluca , Cabau Georgiana , Vulturar Damiana , Chis Ana , Lesan Andrei , Vesa Cristian Stefan , Pop Dana , Todea Doina Adina 
Published: December 2022

AbstractThere is a consistent relationship between obstructive sleep apnea (OSA) and cardiovascular diseases. It is already recognized that OSA may influence the geometry and function of the right ventricle (RV). This has encouraged the development of echocardiographic evaluation for screening of OSA and its severity. Three-dimensional speckle tracking echocardiography (3D STE) is in assumption better, compared with 2D STE, because it overcomes the standard 2D echo limitations. Thus, the purpose of our study is to evaluate whether 3D STE measurements, could predict the positive diagnosis and severity of OSA. We enrolled 69 patients with OSA and 37 healthy volunteers who underwent a cardiorespiratory sleep study. 2DE was performed in all patients. RVEF and 3D RVGLS were measured by 3DSTE. NT pro BNP plasma level was also assessed in all participants. 3D RV GLS (− 13.5% vs. − 22.3%, p < 0.001) and 3D RVEF (31.9% vs. 50%, p < 0.001) were reduced in patients with OSA, compared with normal individuals. 3D Strain parameters showed better correlation to standard 2D variables, than 3D RVEF. Except for NT pro BNP (p = 0.059), all parameters served to distinguish between severe and mild-moderate cases of OSA. 3D STE may be a reliable and accurate method for predicting OSA. Consequently, 3D RV GLS is a good tool of assessing the RV global function in OSA, because it correlates well with other established measurements of RV systolic function. Furthermore, 3D RV GLS was a precise parameter in identifying severe cases of OSA, while NT pro BNP showed no association.