bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocol for the Pathways Study: a realist evaluation of staff social ties and communication in the delivery of neonatal care in Kenya
Authors: Wanyama Conrad , Blacklock Claire , Jepkosgei Juliet , English Mike , Hinton Lisa , McKnight Jacob , Molyneux Sassy , Boga Mwanamvua , Musitia Peris Muoga , Wong Geoff 
Published: March 2023

IntroductionThe informal social ties that health workers form with their colleagues influence knowledge, skills and individual and group behaviours and norms in the workplace. However, improved understanding of these ‘software’ aspects of the workforce (eg, relationships, norms, power) have been neglected in health systems research. In Kenya, neonatal mortality has lagged despite reductions in other age groups under 5 years. A rich understanding of workforce social ties is likely to be valuable to inform behavioural change initiatives seeking to improve quality of neonatal healthcare.This study aims to better understand the relational components among health workers in Kenyan neonatal care areas, and how such understanding might inform the design and implementation of quality improvement interventions targeting health workers’ behaviours.Methods and analysisWe will collect data in two phases. In phase 1, we will conduct non-participant observation of hospital staff during patient care and hospital meetings, a social network questionnaire with staff, in-depth interviews, key informant interviews and focus group discussions at two large public hospitals in Kenya. Data will be collected purposively and analysed using realist evaluation, interim analyses including thematic analysis of qualitative data and quantitative analysis of social network metrics. In phase 2, a stakeholder workshop will be held to discuss and refine phase one findings.Study findings will help refine an evolving programme theory with recommendations used to develop theory-informed interventions targeted at enhancing quality improvement efforts in Kenyan hospitals.Ethics and disseminationThe study has been approved by Kenya Medical Research Institute (KEMRI/SERU/CGMR-C/241/4374) and Oxford Tropical Research Ethics Committee (OxTREC 519-22). Research findings will be shared with the sites, and disseminated in seminars, conferences and published in open-access scientific journals.
ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin 
Published: March 2023

AbstractTumor metastasis is a complex process. A better understanding of the mechanistic underpinnings of the metastatic cascade could uncover approaches to better prevent and target metastases. Here, we integrated transcriptomic profiling in primary esophageal squamous cell carcinoma (ESCC) and matched lymph node metastatic tissues and genome-wide CRISPR/Cas9 functional screening to identify the key drivers of cancer metastasis. Anoctamin 1 (ANO1) expression was found to be elevated in primary tumors and further increased in metastatic tissues. High expression of ANO1 was associated with poor patient survival, and ANO1 overexpression promoted cancer invasion and metastasis. Mechanistically, ANO1 inactivated the LXR pathway and increased intracellular cholesterol accumulation by interacting with JUN to repress the transcription of cholesterol hydroxylase, CYP27A1. In a paracrine manner, IL1β secreted from ANO1-expressing ESCC cancer cells activated NF-κB signaling in fibroblasts to stimulate production of the chemokine CCL1, which in turn enhanced invasion of ESCC cancer cells. Furthermore, molecular docking and in silico screening of 1,500,000 small molecules identified K786–4469 as a lead compound that targeted ANO1 and suppressed cancer metastasis in vitro and in vivo. Overall, these findings uncover a noncanonical function of ANO1 in regulating cholesterol metabolism and fibroblast function, revealing ANO1 as a potential prognostic biomarker and therapeutic target for metastatic ESCC.Significance:Metastatic cancer cells upregulate ANO1 to activate cell-intrinsic and -extrinsic mechanisms that alter cholesterol metabolism and stimulate fibroblasts, which can be targeted with ANO1 inhibitors to inhibit metastatic growth.See related commentary by Singh and Mehla, p. 1759
Comparison of Three Transcytotic Pathways for Distribution to Brain Metastases of Breast Cancer
Authors: Khan Imran , Gril Brunilde , Paranjape Anurag N. , Robinson Christina M. , Difilippantonio Simone , Biernat Wojciech , Bieńkowski Michał , Pęksa Rafał , Duchnowska Renata , Jassem Jacek , Brastianos Priscilla K. , Metellus Philippe , Bialecki Emilie , Woodroofe Carolyn C. , Wu Haitao , Swenson Rolf E. , Steeg Patricia S. 
Published: March 2023

Abstract

Advances in drug treatments for brain metastases of breast cancer have improved progression-free survival but new, more efficacious strategies are needed. Most chemotherapeutic drugs infiltrate brain metastases by moving between brain capillary endothelial cells, paracellular distribution, resulting in heterogeneous distribution, lower than that of systemic metastases. Herein, we tested three well-known transcytotic pathways through brain capillary endothelial cells as potential avenues for drug access: transferrin receptor (TfR) peptide, low-density lipoprotein receptor 1 (LRP1) peptide, albumin. Each was far-red labeled, injected into two hematogenous models of brain metastases, circulated for two different times, and their uptake quantified in metastases and uninvolved (nonmetastatic) brain. Surprisingly, all three pathways demonstrated distinct distribution patterns in vivo. Two were suboptimal: TfR distributed to uninvolved brain but poorly in metastases, while LRP1 was poorly distributed. Albumin distributed to virtually all metastases in both model systems, significantly greater than in uninvolved brain (P &lt; 0.0001). Further experiments revealed that albumin entered both macrometastases and micrometastases, the targets of treatment and prevention translational strategies. Albumin uptake into brain metastases was not correlated with the uptake of a paracellular probe (biocytin). We identified a novel mechanism of albumin endocytosis through the endothelia of brain metastases consistent with clathrin-independent endocytosis (CIE), involving the neonatal Fc receptor, galectin-3, and glycosphingolipids. Components of the CIE process were found on metastatic endothelial cells in human craniotomies. The data suggest a reconsideration of albumin as a translational mechanism for improved drug delivery to brain metastases and possibly other central nervous system (CNS) cancers.
In conclusion, drug therapy for brain metastasis needs improvement. We surveyed three transcytotic pathways as potential delivery systems in brain-tropic models and found that albumin has optimal properties. Albumin used a novel endocytic mechanism.
Bronchial Safety After Pulsed Field Ablation for Paroxysmal Atrial Fibrillation
Authors: Füting Anna , Reinsch Nico , Brokkaar Lenny , Hartl Stefan , Höwel Dennis , Essling Anika , Bell John , Rausch Eva , Neven Kars 
Published: March 2023

Background:
Thermal left atrial ablation can cause bronchial damage. Pulsed field ablation (PFA) is a novel, nonthermal ablation modality for paroxysmal atrial fibrillation. We report on bronchial effects after pulmonary vein isolation using PFA for paroxysmal atrial fibrillation.

Methods:
A computed tomography scan showing the respiratory tract adjacent to the left atrial was obtained. Oral anticoagulation was interrupted on procedure day. Peri-procedurally, patients received heparin with an activated clotting time goal of >350 seconds. All pulmonary veins were individually isolated with a 13F steerable sheath and a pentaspline PFA catheter using either a straight-tip or J-tip guide wire. The J-tip guide wire patients were added to test the hypothesis that the straight-tip guidewire was associated with bleeding complications. One day afterward, bronchoscopy was performed. Serial hemoglobin levels were measured during 30-day follow-up.

Results:
In 2 series of 30 patients, PFA was performed, with all pulmonary veins acutely isolated. Clinical course was uneventful, no patient had chest discomfort, coughing, or hemoptysis. All patients underwent uncomplicated bronchoscopy, without thermal lesions or ulcers. In 12 out of 30 (40%) straight-tip guide wire patients, small amounts of old blood without active bleeding were seen in multiple segments. All hemoglobin levels remained clinically stable. At 30-day follow-up, all patients were asymptomatic.

Conclusions:
Pulmonary vein isolation using PFA for paroxysmal atrial fibrillation does not cause thermal lesions in the bronchial system. Use of a straight-tip, extrastiff guide wire for the over-the-wire PFA catheter can lead to asymptomatic bleeding in the bronchial system without clinical relevance at 30-day follow-up, opposite to use of a J-tip guide wire.
The identity of the freshwater crab Aiyunamon tumidum (Wood-Mason, 1871) (Brachyura, Potamidae), with description of a new species from China
Authors: Pan Da , Sun Hongying , Ng Peter K. L. 
Published: March 2023

Abstract
The identity of the freshwater crab, Aiyunamon tumidum (Wood-Mason, 1871), is clarified after the types are re-examined. The type series is mixed and includes specimens of two species. A syntype from Ponsee in Yunnan, P.R. China, is designated the lectotype of the species; those from Hotha in Yunnan are now referred to A. daiae (Zhang & Sun, in Zhang, Pan, Hao & Sun, 2020). Specimens which have previously been referred to as “A. tumidum” from other parts of Yunnan are here identified as a new species, Aiyunamon fatum sp. nov.. Aiyunamon fatum is most easily distinguished from congeners by the structure of the male first gonopod.
Benjamin Ferencz and the Treatment of Victims in International Criminal Law: Mapping Out Lex Lata and Lex Ferenda (Ferencza?) in an Emerging Field
Authors: Gordon Gregory S. 
Published: March 2023

Abstract
This piece examines a hitherto underexplored legal history chapter in international criminal law pioneer Benjamin Ferencz’s career, and, based on that, offers fixes for problems in current atrocity victim law. Known primarily for his Nuremberg prosecutorial exploits, Ferencz actually spent most of his career innovatively seeking reparations for Holocaust survivors and then later, with the benefit of such experience, sought to ensure coverage of victims in the International Criminal Court’s Rome Statute. After examining this history, the article maps Ferencz’s trailblazing practices onto the atrocity victim lex lata. It then considers that law’s deficits, including front-end and back-end problems (i.e., at the investigation and early release application phases), International Criminal Court retributive versus reparative mission dissonance, inadequate funding, hindrances to proactive victim participation and victim exclusion in prosecuting aggression. For each problem, Ferencz’s history offers viable solutions, such as victim-oriented investigations, bifurcated retribution/restitution processes, bilateral treaty funding, transnational victim networking, and charging illegal use of force as crimes against humanity. As a result, perhaps such proposed modifications of the framework should not be called lex ferenda, but rather ‘lex ferencza.’
Ekklēsia: Contending for Church as ‘People’
Authors: Zarns Phil 
Published: March 2023

Abstract
This article investigates the presumed identity of the present-day Church while seeking parity in terms and meaning with the identity of the early church. The phrase, ‘The church is not a building; the people are the church’, displays the existence of multiple perceived identities of what/who is known as the church within Western culture. New Testament Scripture best defines the church of Jesus Christ as a gathering of believers, not an inert structure. English-speaking contexts utilize church to describe both a community in relationship to God and owned real estate. Identity confusion about what/who the church is affects the perceived agency of the community of faith. This research then proposes cues to define and retain a nt scriptural identity of the church amidst contemporary cultural influence.
On the power of the complaint and the complaints of the disempowered
Authors: Tickoo Asha 
Published: March 2023

Abstract
The objective of this paper is to examine how Chinese learners of EFL frame their complaints on English language learning (ELL) to understand their sense of entitlement to complain. Two basic complaint-frames are identified—complaint proper (Cp), which communicates entitlement in blaming and asking for correction of what is intrinsically correctable, and lament (Lt), which conveys the lack thereof by simply ‘lamenting’ something that cannot be solved. Though Cp and Lt appear, therefore, tied to specific concern-kinds, the inherently addressable and unaddressable, respectively, their actual use depends on the perception of the complaint-concern and the relative power of the complainant. When Cp is selected, subordinate status may require diminished expression of entitlement. This is not achieved by mitigating face-threat. Rather, it is necessary to modify the complaint-framing by selecting more, rather than less, Lt-defining features. Applied to complaints on ELL, in the Chinese context, it is found that this inherently Cp-appropriate, patently addressable problem is only, and always, used with mitigation and/or ‘Lt-ization’ to convey deference and/or disentitlement. In the vast majority, students address limitations in program implementation—in authentic language use, and the opportunity for individual thought, creativity and self-determination—in complaints that suggest disaffection and disenfranchisement.
Off-Vertical Body Orientation Delays the Perceived Onset of Visual Motion
Authors: Chung William , Barnett-Cowan Michael 
Published: March 2023

Abstract
The integration of vestibular, visual and body cues is a fundamental process in the perception of self-motion and is commonly experienced in an upright posture. However, when the body is tilted in an off-vertical orientation these signals are no longer aligned relative to the influence of gravity. In this study, the perceived timing of visual motion was examined in the presence of sensory conflict introduced by manipulating the orientation of the body, generating a mismatch between body and vestibular cues due to gravity and creating an ambiguous vestibular signal of either head tilt or translation. In a series of temporal-order judgment tasks, participants reported the perceived onset of a visual scene simulating rotation around the yaw axis presented in virtual reality with a paired auditory tone while in an upright, supine and side-recumbent body position. The results revealed that the perceived onset of visual motion was further delayed from zero (i.e., true simultaneity between visual onset and a reference auditory tone) by approximately an additional 30 ms when viewed in a supine or side-recumbent orientation compared to an upright posture. There were also no significant differences in the timing estimates of the visual motion between all the non-upright orientations. This indicates that the perceived timing of visual motion is negatively impacted by the presence of conflict in the vestibular and body signals due to the direction of gravity and body orientation, even when the mismatch is not in the direct plane of the axis of rotation.
A semi-thin section technique-based approach to quantify the xylem secondary cell wall deposition process
Authors: Liu Tong-Yan , Ji Chengjun , Tang Zhiyao 
Published: March 2023

Summary
Identification of wood formation, cell wall deposition, lignification, and the maturation process contribute to a better understanding of biomass accumulation processes. Traditional methods for studying xylem development are limited by dyeing effects and discrimination experience, are non-quantitative for the degree of cell wall deposition and lignification, or are unsuitable for broad-leaved trees. In this study, we integrated several already existing methods to improve the discriminative accuracy of the cell development stage and to quantitatively describe the cell wall deposition and lignification degree for both softwood and hardwood tree species. To do this, we collected tree microcores every 7–14 days during a growing season for two species, one conifer (Platycladus orientalis) and one broad-leaved tree (Acer truncatum), in the mountainous areas in Beijing, China. We tracked the xylem development using semi-thin section technology combined with polarization microscopy. This integrated approach allows a quantitative description of the xylem cell wall deposition and lignification process of both hardwood and softwood tree species. This approach can be applied to demonstrate the dynamic process between the cambium layer and the production of wood cells and to describe the cell wall deposition and lignification process of wood cells from generation to maturation. This approach has certain application prospects for exploring scientific issues related to wood formation and accumulation processes in forestry and ecological studies.
The Conceptualization of anger through Metaphors, Metonymies and Metaphtonymies in Jordanian Arabic and English: A Contrastive Study
Authors: Alazazmeh Hadeel Mohammad , Zibin Aseel 
Published: March 2023

Abstract
This study aims to examine the metaphors, metonymies and their interaction in Jordanian Arabic (ja) to conceptualize anger. It also investigates the differences/similarities in the conceptualization of anger in ja and English to determine if culture has a role in its metaphorical and metonymical conceptualization. The study adopts Conceptual Metaphor Theory (cmt) and Cultural Linguistics (cl) as theoretical frameworks to identify and analyze the target metaphors and metonymies. The ja data was collected from two sources: 20 female and male ja native-speaker informants and a Jordanian comedy show called ‘Female’. The English anger metaphors and metonymies were collected from Lakoff and Kövecses’s (1987) study. The qualitative data analysis has revealed that anger is conceptualized in ja through conceptual metaphors with a metonymic basis, pure conceptual metaphors, pure conceptual metonymies and conceptual metonymies with a metaphorical interpretation. The results show that similarities were found in the conceptualization of anger through metaphor and metonymy in both languages which were ascribed to universal embodied cognition. Differences were also detected between the two languages, especially in the use of culture-specific source domains and in the use of metaphtonymies (conceptual metonymies with metaphorical interpretation) that may reflect cultural beliefs related to anger.
Some Afterward Remarks
Authors: Ning Chunyan 
Published: March 2023

Abstract
Science, substantially different from technology and engineering, is to give explanations for why-questions about the Nature, and science of language is to give explanations for why-question about the part of the Nature Noam Chomsky has circumscribed as fl and I-language. His current talk reinforces the conceptual necessity of the separation of the system of thought from externalization, and offers explanations for some in-depth questions concerning with the basic properties of human language, by virtue of a stronger version of smt. In these remarks I present my understanding of the ideas encoded in his development of smt, and tentative solutions to some of the unsolved problems or mysteries.
Outcomes for children and adolescents with Juvenile idiopathic arthritis addressed by occupational therapy services in specialist rheumatology clinics in South Africa
Authors: Moodley Darrel , Jacobs Lizelle , Franzsen Denise , de Witt Patricia 
Published: March 2023

Introduction: International guidelines for best practice indicate specialised treatment is required for Juvenile idiopathic arthritis (JIA). While occupational therapy intervention for functional limitations in this chronic condition is conditionally recommended, the extent to which this is provided in public specialist rheumatology clinics in South Africa is unknown. The aim of this study was to determine which needs related to outcomes for occupational performance or functional status, condition-related factors and health-related quality of life, reported by children and adolescents with JIA were addressed by current occupational therapy services. Methods: The study used two descriptive, quantitative studies with limited samples of 37 clients with JIA attending specialist rheumatology clinics and nine occupational therapists who currently provide intervention for clients with JIA. Results: Functional deficits reported by 30–63% of clients with JIA were increased symptoms of active disease and difficulties with school activities. Occupational therapy services addressed condition-related factors and participation in personal management but not many related to the social, educational and psychosocial needs of clients with JIA. Conclusion: Occupational therapy services followed international guidelines but based on the reductionist biomedical model approach used within specialist services in tertiary hospitals, which limit context-based therapy for children and adolescents with JIA in the community.
Evaluation of triacetin on mechanical strength and free surface energy of PHBHHx: The prevention of intra-abdominal adhesion
Authors: Akkurt Yıldırım Meryem , Demirbilek Murat , Kızılbey Kadriye , Kaplan Engin , Türkoğlu Nelisa 
Published: March 2023

Several polymers are used for the preparation of biomaterials as membranes and films for tissue engineering applications. The most common plasticizer is PEG to obtain polymer-based biomaterials. On the other hand, triacetin is a non-toxic, FDA-approved plasticizer mostly used in the food industry. In this study, we used triacetin as a plasticizer to obtain hydrophobic membranes for the prevention of intra-abdominal adhesion. We selected a well-known polymer named PHBHHx which is a bacterial polyester generally used as supporting material for cell attachments in regenerative tissue applications. We evaluated the triacetin as a plasticizer and its effect on mechanical, thermal, surface area, pore size, and surface energy. The hydrophobic/hydrophilic contrast of a biomaterial surface determines the biological response. Surface hydrophobicity is critical for the cellular response. The contact angle tests of PHBHHx revealed that the hydrophilicity of the membrane was decreased following triacetin blending. Modification of the PHBHHx membrane by blending with triacetin caused a significant decrease in cell adhesion. The cell attachment rates of PHBHHx membranes were as 95 ± 5% on the first day, 34.5 ± 0.9% on third day, and 23 ± 1.5% on the fifth day, respectively. The rates of cell attachments on PHBHHx/triacetin membranes were determined as 79 ± 2.5% for the first day, 33 ± 2.7% for the third day, and 13 ± 2.1% for the fifth day, respectively. Besides, triacetin blending decreased the surface area from 38.790 to 32.379 m2/g. The elongation at breaks was observed as 128% for PHBHHx and 171% for PHBHHx/triacetin. Graphical abstract [Formula: see text]