bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycan strand cleavage by a lytic transglycosylase, MltD contributes to the expansion of peptidoglycan inEscherichia coli
Authors: Kaul Moneca , Meher Suraj Kumar , Reddy Manjula 
Published: May 2023

ABSTRACTPeptidoglycan (PG) is a protective sac-like exoskeleton present in most bacterial cell walls. It is a large, covalently cross-linked mesh-like polymer made up of several glycan strands cross-bridged to each other by short peptide chains. Because PG forms a continuous mesh around the bacterial cytoplasmic membrane, opening the mesh is critical to generate space for the incorporation of new material during its expansion. InEscherichia coli, the ‘space-making activity’ is known to be achieved by cleavage of cross-links between the glycan strands by a set of redundant PG endopeptidases whose absence leads to rapid lysis and cell death. Here, we demonstrate a hitherto unknown role of glycan strand cleavage in making space for cell wall expansion inE. coli. We find that overexpression of a membrane-bound lytic transglycosylase, MltD that cuts the glycan polymers of the PG sacculus rescues the cell lysis caused by the absence of essential cross-link specific endopeptidases, MepS, MepM and MepH. Further detailed genetic and biochemical analysis revealed that MltD works in conjunction with cross-link specific endopeptidases to expand the PG sacculus. Interestingly, we find that cellular MltD levels are stringently controlled by two independent regulatory pathways. MltD undergoes regulated proteolysis by NlpI-Prc, a periplasmic adaptor-protease complex that specifically degrades two of the elongation-specific endopeptidases, MepS and MepH. In addition, MltD levels are post-transcriptionally controlled by RpoS, a stationary-phase specific sigma factor. Overall, our results show that coordinated cleavage of the glycan strands and the peptide cross-bridges facilitates the opening of the PG mesh for successful expansion of the cell wall during growth of a bacterium.AUTHOR SUMMARYMost bacteria are protected by a cell wall made up of peptidoglycan (PG), a mesh-like large polymer. PG consists of several linear glycan strands interlinked through short peptide chains to form a continuous meshwork around the bacterial cytoplasmic membrane. Because PG tightly encases the cytoplasmic membrane, the growth of a bacterial cell is coupled to the expansion of PG requiring the activity of hydrolytic enzymes that cleave PG cross-links to make space for incorporation of new PG material. lnE. coli, a set of redundant cross-link specific endopeptidases are known to be crucial for expansion of PG. In this study, we show that cleavage of the glycan polymers by MltD, a glycan cleaving enzyme compensates the absence of cross-link cleavage and contributes to the expansion of PG. Overall, our work shows a previously unknown role of glycan hydrolases in cell wall expansion identifying these as potential targets for development of cell wall-specific antimicrobial agents.
Limits and potential of organic farming towards a more sustainable European agri-food system
Authors: Calabro Grazia , Vieri Simone 
Published: May 2023

PurposeThe aim of this paper is to assess whether the current European target to increase the areas under organic farming to 25% by 2030 is attainable and whether the simple increase in areas under organic farming may be sufficient to improve the sustainability of European agriculture.Design/methodology/approachThe analysis has been carried out through a simple data processing related to areas under organic farming, for the period 2012–2020 (Eurostat database), in order to highlight the trends of areas under organic farming and to verify whether the annual average change rates may be compatible with the stated target.FindingsThe analysis showed that organic farming has a productive weight not corresponding to the amount on the total of the areas under cultivation and a small impact on the total of food consumption. It is a plausible hypothesis, the one that shows the increase in areas under organic farming will engage forms of agriculture and farms that, already, are more sustainable, so the achievement of 25% target will not particularly impact the European potential productive and the less environmental sustainable forms of agriculture.Originality/valueThis paper contributes to the debate, involving scientific community, policy maker and civil society, about the real contribution of organic farming to sustainability, and it will be developed in future research.
A model of image retrieval based on KD-Tree Random Forest
Authors: Dinh Nguyen Thi , Nhi Nguyen Thi Uyen , Le Thanh Manh , Van Thanh The 
Published: May 2023

PurposeThe problem of image retrieval and image description exists in various fields. In this paper, a model of content-based image retrieval and image content extraction based on the KD-Tree structure was proposed.Design/methodology/approachA Random Forest structure was built to classify the objects on each image on the basis of the balanced multibranch KD-Tree structure. From that purpose, a KD-Tree structure was generated by the Random Forest to retrieve a set of similar images for an input image. A KD-Tree structure is applied to determine a relationship word at leaves to extract the relationship between objects on an input image. An input image content is described based on class names and relationships between objects.FindingsA model of image retrieval and image content extraction was proposed based on the proposed theoretical basis; simultaneously, the experiment was built on multi-object image datasets including Microsoft COCO and Flickr with an average image retrieval precision of 0.9028 and 0.9163, respectively. The experimental results were compared with those of other works on the same image dataset to demonstrate the effectiveness of the proposed method.Originality/valueA balanced multibranch KD-Tree structure was built to apply to relationship classification on the basis of the original KD-Tree structure. Then, KD-Tree Random Forest was built to improve the classifier performance and retrieve a set of similar images for an input image. Concurrently, the image content was described in the process of combining class names and relationships between objects.
Leadership style, knowledge sharing and audit quality
Authors: Hassanzadeh Mohassel Ali , Hesarzadeh Reza , Bagherpour Velashani Mohammad Ali 
Published: May 2023

PurposeThis paper aims to examine how leadership style in audit firms influences audit quality. The paper further explores the mediating role of knowledge sharing in the relationship between leadership style and audit quality.Design/methodology/approachThe present paper studies the effects of transformational and servant leadership styles on audit quality through knowledge sharing. Data are collected from 396 Iranian external auditors via a questionnaire.FindingsThe results show that both transformational and servant leadership style significantly influence audit quality through knowledge sharing. Moreover, the impact of transformational leadership style is stronger than the impact of servant leadership style.Originality/valueIn audit quality literature, little attention has been devoted to both leadership style and knowledge sharing. This paper develops a parsimonious model which shows how leadership style improves audit quality, and how knowledge sharing strengthens the impact of leadership style on audit quality. The results have important implications particularly for audit industry.
Mediating effect of integrated health commodities procurement system on the relationship between responsiveness and health service delivery
Authors: Israel Baraka 
Published: May 2023

PurposeThe problems that face health service delivery across different countries are compounded by financial, political, institutional and technical deficiencies. Yet, the role of technological aspects in the procurement of health commodities and health service delivery system requires in-depth exploration. This study bridges this gap by examining the mediating effect of an integrated health commodities procurement system on the relationship between responsiveness and health service delivery.Design/methodology/approachData for this study were collected from 274 respondents, comprising procurement staff and pharmacists using a cross-sectional questionnaire survey. A total of 28 government-owned hospitals from 6 regions in the Southern Highland of Tanzania were sampled for observation. Confirmatory factor analysis (CFA) and structural equation modelling (SEM) were used for data analysis.FindingsThe results of the study revealed a positive and significant relationship between responsiveness and integrated health commodities procurement system (β = 0.572, p < 0.001). Responsiveness positively and significantly affects health service delivery (β = 0.175, p = 0.004). The results also show that integrated health commodities procurement system is positive and significantly related to health service delivery (β = 0.264, p < 0.001). Lastly, the bootstrapping confidence intervals revealed that an integrated health commodities procurement system significantly mediates the relationship between responsiveness and health service delivery.Practical implicationsTo strengthen the health service delivery system, the study recommends enforcing internal control mechanisms and supporting policies that will monitor and evaluate the effectiveness of the integrated health commodities procurement system and service practitioners' responsiveness. Moreover, health service managers should ensure that the planning, procurement and distribution of health commodities are fully and effectively integrated at each node of the health supply chain.Originality/valueThe study contributes to the body of knowledge which examines the efficacy of health service delivery from procurement perspective. To the best of the author's knowledge, this is the first study that offers empirical evidence for the mediating effect of integrated health commodities procurement system on the link between responsiveness and health service delivery.
The dual institutional work of Lyra's Walk: partisan violence and peace protest in Northern Ireland
Authors: Gidley Devon , Lubit Amanda J. 
Published: May 2023

PurposeThe purpose of this paper is to explore peace protest as a form of institutional work aimed at supporting one institution and disrupting another.Design/methodology/approachThe authors utilized walking ethnography (28 miles in 18 h while conducting 25 walking interviews) and digital media analysis (news reports, social media and electronic communication).FindingsWalking participants engaged in multiple types of institutional work aimed at maintaining the Good Friday Agreement and disrupting partisan violence. The institutional work left no lasting impact on either institution.Originality/valueThe paper conceptualizes two competing institutions and situates the dual institutional work of Lyra's Walk in the post-conflict context of Northern Ireland. The study contributes to understanding formality and multiplicity in institutional work research.
Emergency plan selection for epidemic prevention and control based on cumulative prospect theory and hybrid-information MADM
Authors: Mao Qinghua , Chen Jinjin , Lv Jian , Chen Shudong 
Published: May 2023

PurposeDecision-making problems in emergency plan selection for epidemic prevention and control (EPAC) are generally characterized by risky and uncertainty due to multiple possible emergency states and vagueness of decision information. In the process of emergency plan selection for EPAC, it is necessary to consider several obvious features, i.e. different states of epidemics, dynamic evolvement process of epidemics and decision-makers' (DMs') psychological factors such as risk preference and loss aversion.Design/methodology/approachIn this paper, a novel decision-making method based on cumulative prospect theory (CPT) is proposed to solve emergency plan selection of an epidemic problem, which is generally regarded as hybrid-information multi-attribute decision-making (HI-MADM) problems in major epidemics. Initially, considering the psychological factors of DMs, the expectations of DMs are chosen as reference points to normalize the expectation vectors and decision information with three different formats. Subsequently, the matrix of gains and losses is established according to the reference points. Furthermore, the prospect value of each alternative is obtained and the comprehensive prospect values of alternatives under different states are calculated. Accordingly, the ranking of alternatives can be obtained.FindingsThe validity and robustness of the proposed method are demonstrated by a case calculation of emergency plan selection. Meanwhile, sensitivity analysis and comparison analysis with fuzzy similarity to ideal solution (FTOPSIS) method and TODIM (an acronym in Portuguese for interactive and MADM) method illustrate the effectiveness and superiority of the proposed method.Originality/valueAn emergency plan selection method is proposed for EPAC based on CPT, taking into account the psychological factors of DMs.HighlightsThis paper proposes a new CPT-based EDM method for EPAC under a major epidemic considering the psychological factorsof DMs, such as risk preference, loss aversion and so on.The authors' work gives approaches of normalization, comparison and distance measurement for dealing with the integration of three hybrid formats of attributes.This article gives some guidance, which contributes to solve the problems of risk-based hybrid multi-attribute EDM.The authors illustrate the advantages of the proposed method by a sensitivity analysis and comparison analysis with existing FTOPSIS method and TODIM method.
Market exit and re-entry in a volatile emerging economy: a case study of Yamaha motorcycles in Pakistan
Authors: Khan Huda , Zahoor Nadia , Arslan Ahmad , Khan Zaheer 
Published: May 2023

Purpose
This study aims to understand the dynamics underpinning the exit and re-entry strategies adopted by multinational enterprises (MNEs) in an emerging market, Pakistan.

Design/methodology/approach
This study undertook an in-depth historical case study of Yamaha Motorcycles, which had initially entered Pakistan as a joint venture but had then exited and re-entered as a wholly owned subsidiary.

Findings
This study found that, despite its status as a market leader and one of the older players in the Pakistani market, changing market dynamics in the 2000s – especially the increased competition brought by more affordable (inexpensive) Chinese motorcycles and the weak enforcement of industrial policies – had pushed Yamaha Motorcycles to exit. Another factor that had contributed to its exit were differences in risk perception and strategies with its local joint venture partner (a Pakistani business group). Hence, both firm-level and institutional factors had played significant roles in Yamaha’s market exit. This study further found that re-entering in a wholly owned subsidiary operation mode had been beneficial for the firm, as it gained a significant market share due to its focus on innovation and on capturing a market niche, which had earlier not been its main focus. The findings also suggest that opportunity logics and multiple forms of learning can be important for a firm’s re-entry into a host market – such as experiential (i.e. learning from experience) and vicarious learning (i.e. learning from other organizations, including suppliers and competitors) in an emerging market context, in which institutions evolve amid political and policy uncertainty. Finally, this study found that exit and re-entry timing is an important factor for the development of competitive advantage in a host market.

Originality/value
This study is among the few to have investigated the exit and re-entry strategies of MNEs in emerging markets. The relatively short time during which Yamaha Motorcycles had been out of the market had benefited it on its re-entry, as the firm had been able to capitalize on its prior learning and ties to suppliers’ networks.
Reducing anxiety and hospital readmission in the early weeks following discharge from inpatient rehabilitation: the new community enhanced rehabilitation team
Authors: Fenton Kelly , Kidd Katherine , Lord Alex 
Published: May 2023

Purpose
The purpose of this study is to assess if the new community-enhanced rehabilitation team reduced anxiety and readmissions in service users discharged from an inpatient rehabilitation setting.

Design/methodology/approach
The study used both qualitative and quantitative methodology. Service user’s anxiety level was measured before being discharged and at the end of the Community Enhanced Rehabilitation Team (CERT) transition intervention. Six service users were interviewed to gain further understanding of their experiences of anxiety.

Findings
Findings showed the anxiety score was significantly lower (M = 1.5, 95% CI [0.051,2.99], t(20) = 2.159, p = 0.043) following the CERT intervention (M = 8.6, SD = 6.4) compared to before (M = 10.1, SD = 7.0). No service user receiving the CERT intervention was readmitted to hospital within 12 weeks of discharge from the inpatient setting, compared to three service users (15% of those discharged) who were discharged to other community services.

Research limitations/implications
Community rehabilitation pathways would benefit from having interventions to aid patient transitions from inpatient to the community. The National Health Service (NHS) trusts develop community rehabilitation teams as recommended by the National Institute for Health and Care Excellence (NICE), and they should consider including transitional support as part of their model.

Practical implications
It is recommended that as NHS trusts design and implement community mental health teams, they should consider including transition support as part of their model.

Social implications
People with severe and enduring mental health difficulties who have been in an inpatient rehabilitation setting would benefit from community transitional support. This study suggests that such support helps reduce anxiety and readmission.

Originality/value
Community rehabilitation teams are currently being developed across the NHS as part of the NHS long-term plan. These teams are new, and as such, there is a dearth of information regarding their effectiveness. To the best of the authors’ knowledge, this is one of the first studies to evaluate outcomes in these new teams.
Knowledge management through information and communication technology investments: the story of Indian family firms
Authors: Mondal Arindam , Chakrabarti Amit Baran 
Published: May 2023

PurposeInformation and communication technologies (ICT) are indispensable tools for Knowledge Management (KM) practices in today’s knowledge-intensive and globally interconnected marketplace. This paper seeks to investigate the impact of family ownership on ICT investments in an emerging economy (EE) context.Design/methodology/approachThis empirical paper uses data from 300 large Indian listed firms with 2,650 observations in the period 2008–2017, to test its hypothesis.FindingsThe results indicate that family firms are not favourably inclined towards ICT investments for formalizing their KM practices. However, under certain contexts, such as higher foreign institutional ownership or business group affiliation, they are more willing to invest in ICT resources.Practical implicationsThis study establishes a nuanced understanding of how family firms approach ICT investments and KM practices. This research can help family owners/managers to commit sufficient resources on ICT projects.Originality/valueLiterature on KM has largely emanated from developed countries. This is one of the first papers from an EE context that studies the impact of family ownership on ICT investments and subsequent KM practices. In this way, this paper offers specific insights into the context of Indian family firms and offers some interesting findings that can contribute to the literature, policy and practice.
The role of online learning in enhancing the performance of introverted female Saudi students in translation
Authors: Alaskar Hajer 
Published: May 2023

PurposeThe purpose of the current study was to examine the role of distance learning in enhancing introverted students’ lack of communication and social interaction to improve their performance in translation class. Cain (2013) and Kuzeljevich (2017) agreed that identifying “introverted” and “extroverted” students is important for meeting their learning needs. While extroverted students have strong social skills that allow them to interact comfortably in different learning environments, introverted students tend to be more shy, quiet, and silent, thus, requiring more careful planning in classroom settings. Therefore, educators need to support introverted students in reaching their full academic and social potential.Design/methodology/approachThe present case study adopted a qualitative research method to explore the role of online/distance learning during the COVID-19 pandemic in enhancing introverted students’ performance and communication abilities in translation classes. The researcher of the current study spent a considerable time observing and set herself as part of the group (i.e. translation students of level 6 class) to understand the phenomenon, events and the new situation of having translation students interact in online settings. Data collection was based on this observation, interviews with the participants and archival documents. To enhance the validity and credibility of this research, the researcher employed the method of triangulation.FindingsThe results (see Appendixes A, B and C) revealed the level of students interactions in translation classes and their attitudes toward online learning. Based on the observations made by the instructor, the researcher found that the involvement of the introverted students during online translation learning was remarkable, as they provided their translation outputs in the chat window of Microsoft Teams with no hesitation. Consequently, 65% of the students were providing their translation output through the chat window, which indicates that they are more introverted and preferred not to speak. Comparing this result to face-to-face translation class, the researcher found that 25% of the students provided their translation outputs through oral participation.Originality/valueThis study contributes to the field of translation and education. Previous studies have not sufficiently examined the role of distance learning in enhancing the performance and communication of introverted students in translation classes. The current study is also expected to provide insight into the field of technical translation in remote teaching and learning settings.