bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling and statistical study of a DBN for Cloud and Cloudlet environment within IoT technologies
Authors: KORTAS Nawel , YOUSSEF Habib 
Published: March 2023

Abstract
The IoT has generated an amount of data that puts enormous pressure on the internet infrastructure. Therefore, companies are scrambling to find ways to alleviate this pressure and solve the data problem. Cloud Computing plays a major role in this, in particular by making all connected devices work together. Virtual machine (VM) migration concept and the architecture of datacenters for cloud computing have a significant impact on latency and energy cost. In order to lower latency and energy usage while maintaining SLA restrictions, this work focuses on how to employ VM migration to achieve steady physical machine use. Using the novel MVMM scheduler method for virtual machine migration, we suggest modeling and statistical analysis of a Dynamic Bayesian Network for cloud and cloudlet environments within IoT technologies. It use a Dynamic Bayesian Network study to decide where and when a particular VM migrates. Indeed, to improve latency and energy consumption by limiting the number of upcoming migrations, the statistical study computes a score for each VM candidate for migration using datacenter metrics as input. In addition, this paper focuses to set solution of network latency problem for communicating objects in IoT technologies by the use of Cloudlets environment by proposing a conception of a local datacenter that allows users to connect to their data from any point and through any devices. The performance demonstration results for resource utilization rate, average execution time, and lost packets show that cloudlet applications have a significant advantage over local and remote connections.
MTHFR act as a potential cancer biomarker in immune checkpoints blockades, heterogeneity, tumor microenvironment and immune infiltration
Authors: Wu Zhongjun , Peng Jianheng 
Published: March 2023

Abstract
Purpose To evaluate the role and landscape of 5-10-Methylenetetrahydrofolate reductase (MTHFR) to immune infiltration, tumor microenvironment, heterogeneity, immune checkpoints blockades, prognostic significance across cancer types.
Methods Data sets of genomic, transcriptomic and clinic features of MTHFR across >60,000 patients and up to 44 cancer types were comprehensively analyzed using R software.
Results Expression of MTHFR gene is significantly lower in 17 tumors and correlated with OS, DSS, PFI in specific tumors. Gene alterations of MTHFR are observed significant differences across tumor types. Expression of MTHFR is negatively correlated with the mDNAsi, mRNAsi, DMPsi, ENHsi, EREG-mDNAsi and EREG-mRNAsi in the most cancers. MTHFR showed significantly correlated with 67 types of immune cell infiltration scores in 44 cancer types by XCELL algorithm. GO and KEGG enrichment analysis are conducted to show the core tumor mechanism and biological process. Correlations between MTHFR and biomarkers of heterogeneity (MSI, TMB, MATH, HRD, LOH, Neoantigen, ploidy and purity) are also significant in specific tumors. MTHFR is significantly positively correlated with biomarkers of immune related genes (CD19, CD274, CD80, CD86) and mismatched repair genes (MLH1, PMS2, MSH2, MSH6, EPCAM, MLH3, PMS1, EXO1) in most cancer types. ROC analyses show MTHFR could act as a biomarker in anti-PD1 and anti-CTLA4 group of ontreatment, in anti-PD1 (pembrolizumab) group in pretreatment. Two immunohistochemistry antibodies HPA076180 and HPA077255 are verified in 20 types of tumor and could be used to detect the expression of MTHFR efficientlyin clinic.
Conclusions MTHFR could predict the response of immune checkpoints blockades, heterogeneity, tumor microenvironment and immune infiltration.
CCL5 Promotes LFA-1 Expression in Th17 Cells and Induces LCK and ZAP70 Activation in a Mouse Model of Parkinson’s Disease
Authors: Zhao JingWei , Mao Zhijuan , Yi Qu , Wang Danlei , Li Jiangting , An Ke , Min Zhe , Xue Zheng 
Published: March 2023

Abstract
Background: Parkinson's disease (PD), the second most common neurodegenerative disease, is characterized by the pathological deposition of alpha-synuclein (α-Syn) and loss of dopaminergic (DA) neurons. Th17 cells are considered to be responsible for the direct loss of DA neurons. C-C chemokine ligand 5 (CCL5) specifically induces Th17 cell infiltration in the substantia nigra (SN). However, the specific effect of CCL5 on Th17 cells in PD and the relationship between CCL5 and lymphocyte function-associated antigen-1 (LFA-1) expression in Th17 cells have not been clarified.
Methods: We evaluated the effects of CCL5 on LFA-1 expression in Th17 cells in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Moreover, we examined Th17 cell differentiation upon CCL5 stimulation in vitro. Furthermore, we assessed the effects of CCL5 on tyrosine kinase zeta-chain-associated protein kinase 70 (ZAP70) and lymphocyte-specific protein tyrosine kinase (LCK) activity in CCL5-stimulated Th17 cells in vivo and in vitro.
Results: CCL5 increased the proportion of peripheral Th17 cells in MPTP-treated mice, LFA-1 expression on Th17 cells, and the number of Th17 cells in the SN of MPTP-treated mice. Additionally, CCL5 promoted Th17 cell differentiation and LFA-1 expression in naive T cells cultured in vitro. Moreover, CCL5 increased the differentiation of Th17 cells and the expression of LFA-1 by stimulating LCK and ZAP70 activation in naïve CD4+ T cells. Furthermore, inhibiting LCK and ZAP70 activation reduced the proportion of peripheral Th17 cells and surface expression of LFA-1 in MPTP-treated mice, and the number of Th17 cells in the SN was also significantly decreased.
Conclusion: CCL5, which could increase the differentiation of Th17 cells and LFA-1 protein expression by activating LCK and ZAP70, could increase Th17 cells in the SN, induce the death of DA neurons and aggravate PD.
3D printing assisted MIPO for treatment of complex middle-proximal humeral shaft fractures
Authors: Hu Chaoran , Cao Yongfei , Cen Chaode , Luo Qin , Qiu Bing 
Published: March 2023

Abstract
Background To explore the clinical efficacy of 3D printing assisted MIPO technique for treating complex middle-proximal humerus fractures(AO 12C fracture type).
Methods The data of 42 participants who received a complicate middle-proximal humerus fracture from the beginning of 2018 to the end of 2021 were retrospectively analyzed. All patients were assigned to two groups: minimally invasive percutaneous plate osteosynthesis (MIPO) technique with detailed preoperative planning assisted by 3D printing (group A), and traditional MIPO (group B).
Results All patients were followed-up for 8-24 months (mean: 16.12±4.13 months). There were obvious differences found in the operation time, c-arm usage time and intraoperative blood loss between the two groups. However, no significant differences were observed in the range of shoulder joint motion (ROM), the occurrence of complications (surgical incision site infection, flexible internal fixation, bone nonunion and radial nerve palsy), QuickDASH score and Constant score between the two groups.
Conclusion Preoperative 3D printing assisted MIPO technique can reduce the operation time, and exhibits obvious advantages in terms of operational efficiency, which can be applied to treat complex middle-proximal humeral fractures.
Circular wood use can accelerate global decarbonisation but requires cross-sectoral coordination
Authors: Forster Eilidh , Healey John R. , Styles David , Newman Gary 
Published: March 2023

Abstract
Productive forestry can deliver multifaceted climate-change mitigation benefits, including carbon sequestration and avoided emissions from fossil-fuel-derived product substitution. However, predominantly linear use of wood substantially limits the potential mitigation contribution of forestry value-chains. Using lifecycle assessment, we show that more circular and cascading use of wood can provide immediate and sustained climate change mitigation by reducing demand for virgin wood, increasing carbon sequestration and storage, and reducing greenhouse gas emissions. Despite the clear benefits of implementing circular economy principles in forestry value-chains, we identify many functional barriers impeding the structural reorganisation needed for such complex system change. A mind-set shift towards knowledge sharing and collaboration is fundamental to transform the value-chain into an effective societal change system and lead to coherent action; with trade organisations performing important advocacy and organising functions, and governments driving wood flow transparency to reveal efficiency and resourcing opportunities.
ELISA Assay for estimation of Prednisolone Drug Using Bridge Heterology in Enzyme Conjugates
Authors: Sonkar Subash , Kumar Dinesh , Oberoi Harinder Singh , Singh Harpal , Shrivastav T.G , Bhukya Prudhvi Lal , Kumari Mansi 
Published: March 2023

Abstract
The introduction of spacers in coating steroid protein complex and / or enzyme conjugates or immunogen is known to exert an influence on the sensitivity of steroid enzyme immunoassays. We have introduced different homobifunctional spacers between enzyme and prednisolone (PSL) moiety having atomic length 3 to 10 and studied their influence on functional parameters such as sensitivity and specificity of PSL enzyme immunoassays. In this study, four enzyme conjugates of PSL-21-HS and HRP were prepared by N-Hydroxy Succinimide mediated carbodiimide reaction, these were PSL-21-HS-adipic acid dihydrazide (ADH)-HRP, PSL-21-HS- carbohydrazide (CH)-HRP, PSL-21-HS-ehylenediamine (EDA)-HRP and PSL-21-HS- urea (U)-HRP. The assays were developed using these enzymes conjugate with antibody raised against PSL-21-HS-BSA immunogen. The sensitivity of the PSL assays after insertion of bridge in enzyme conjugate were 1.22 ng/mL, 0.59 ng/mL, 0.48 ng/mL and 0.018 ng/mL with ADH, CH, EDA and urea as spacer respectively. Among all four combinations, PSL-21-HS-BSA antibody and PSL-21-HS-U-HRP enzyme conjugate gave better sensitivity and showed cross-reaction with less number of steroids. The percent recovery of PSL from the exogenously spiked human serum pools was in the range of 88.32-102.50 %. The intra and inter assay CV % was < 8.46%. The PSL concentration was estimated in the serum samples of patients on PSL treatment. The serum PSL values obtained by this method correlated well with the commercially available kit (r2 = 0.98). The present study, suggests that the nature of the spacer is related to assay sensitivity and not the spacer length.
The Role of the Notary when Making a Canceled Purchase Agreement Deed in Court (Review of Articles 15 and 16 of the Indonesian Notary Law)
Authors: Prasetyo Danang Hani , Prihatinah Tri L. , . Sulistyandari 
Published: March 2023

A notary deed is an authentic deed drawn up by or before a notary according to the form and procedure stipulated in Law Number 2 of 2014 concerning the Office of a Notary. Article 15 of the law on the position of a notary regulates the authority of a notary, and Article 16 of the law on the position of a notary regulates the obligations of a notary. In carrying out these powers and obligations, a role is required in the form of a series of actions that are supportive in carrying out the duties of a notary in accordance with his position if the Notary does not play a role in causing the deed to be canceled, as in the Supreme Court Decision No. 535 PK/Pdt/2017 (1) the cancellation was due to an imbalance between what was written in the sale and purchase agreement deed and the facts of the parties. The decision of the Surabaya District Court (2) stated that the deed made by a notary was valid according to legal provisions. In contrast, the decision of the Surabaya High Court (3) stated that the deed was legally flawed. The decision of the Supreme Court (4) at the cassation and review level upheld the decision. This study analyzes the juridical factors that cause the deed of sale and purchase agreement made before a notary to be void based on a court decision and analyzes the role of the Notary in making a sale and purchase agreement deed. The research method used is normative juridical, using secondary data obtained from literature studies, including primary, secondary, and tertiary legal sources. The juridical factor that caused the cancellation of the sale and purchases agreement deed by the Notary, according to the Supreme Court's decision upholding the high court's decision, was because the plaintiff of the convention could prove the argument for his lawsuit regarding bilyet giro not for land payment in the sale and purchase agreement deed but for the sale and purchase of scrap metal where the plaintiff (seller) is an intermediary in buying and selling scrap metal. The Notary has yet to play a role in making the sale and purchase agreement deed, which resulted in the sale and purchase agreement deed being canceled based on a court decision. The problem is caused by the Notary not carrying out roles, such as providing legal counseling in connection with doing the deed as stipulated in the notary position law Article 15 paragraph (2) letter (e), carrying out the task carefully as stipulated in Article 16 paragraph (1) letter a, Reading the deed before the parties attended by at least 2 (two) witnesses as referred to in Article 16 paragraph (1) letter (m).
Ukraine’s Integration into the European Social Space: Problems and Prospects
Authors: Protsiuk Ihor , Boichuk Dmytro , Chyzhov Denys 
Published: March 2023

The article is devoted to the analysis of the essence and purpose of the social state, as a state of general well-being, which is one of the key aspects of the perception of the state based on the principle of the rule of law (rule of law). The subject of the research is the following categories of jurisprudence: social legal state, European social model, European social space, social policy of the state. The purpose of the article is to analyze the main features of the social state as a model of the ideal state, the social policy of the state, the European social model, domestic achievements, problems and providing proposals for the fastest entry of Ukraine into the European social space. The research uses dialectical, systemic, structural-functional, logical, historical, comparative-legal, theoretical modeling and other methods traditional for jurisprudence. Some aspects of the development of social statehood in Ukraine are studied, namely the activity of such an institution of civil society as the volunteer movement and its influence on the formation of such a state, as well as the implementation of the social function of the state in relation to persons with disabilities. The experience of the European Union in the spheres of social protection and environmental human rights is considered. The need for further adaptation of the main foundations of the country's social direction of our state to the European social space has been identified. Based on the conducted research, the following conclusions were formulated: the welfare state marks a certain historical stage in the development of the concept of a democratic, legal state; the functioning of such a state, at the current stage of society's development, is possible in the presence of an appropriate model of the social market economy, which ensures the stability of modern society and its sustainable development; the improvement of the legal foundations of the social state at the current stage should take place taking into account the tendency to "green" the democratic, legal state, as well as the formation of the European social model and integration into the European social space; within the social sphere of such a state, the implementation of socio-economic and environmental rights enshrined at the constitutional level should be guaranteed. Recommendations are given on borrowing the experience of European countries in building a social state in Ukraine, ensuring the rights of vulnerable categories of Ukrainian citizens, along with state authorities and local self government bodies, as well as civil society institutions.
Do Pleiotropic Effects of Spironolactone in Women with PCOS Make it More than
an Anti-androgen? Evidence from a Systematic Review and Meta-analysis
Authors: Ganie Mohd. Ashraf , Bashir Rohina , Asrar Mir Mahmood , Shah Idrees Ayoub , Wani Imtiyaz Ahmad 
Published: March 2023

Background:
Spironolactone use as a treatment for hirsutism and other dermatological conditions among polycystic ovary syndrome (PCOS) and idiopathic hirsutism shows varied results.

Objective:
This study thus summarizes the entire evidence to better define its impact on Ferriman-Gallwey (FG) score in addition to other derangements associated with PCOS.

Methods::
PubMed, Embase, Scopus and bibliographies of relevant articles were searched. RCTs investigating the efficacy of spironolactone in PCOS and idiopathic hirsutism were included. Pooled mean difference (MD) was calculated using random effects model and relevant subgroup analysis was done. Potential heterogeneity and publication bias was assessed.

Results:
Of 1041 retrieved studies, 24 RCTs were included. Spironolactone (100mg/daily) exhibited a significant reduction in FG score in idiopathic hirsutism compared to finasteride [MD: -2.43; 95% C.I:(-3.29, -1.57)] and cyproterone acetate [MD: -1.18; 95% C.I:(-2.10, -0.26)], however, no significant difference was found among PCOS subjects in comparison to flutamide and finasteride. A lower dose of spironolactone (50mg/day) exhibited no significant difference relative to metformin on FG Score [MD: -0.61; 95% C.I: -1.76, 0.54,I2=57%)], serum total testosterone [MD: -0.61; 95% C.I: -1.76, 0.54), I2= 57%] and HOMA-IR [MD: 1.03; 95% C.I: -1.22, 3.29), I2=60%] among PCOS women. The main side effects reported by the studies were menstrual irregularity, mild nausea, vomiting and diarrhea.

Conclusion:
Spironolactone is well tolerated among idiopathic hirsute and PCOS women. The drug significantly improved hirsutism in the former group and shows a positive trend in the latter women, however, displays no effect on FSH, LH, menstrual cyclicity, BMI, and HOMA-IR in PCOS women.
Collecting Food and Drink Intake Data With Voice Input: Development, Usability, and Acceptability Study
Authors: Millard Louise A C , Johnson Laura , Neaves Samuel R , Flach Peter A , Tilling Kate , Lawlor Deborah A 
Published: March 2023

Background
Voice-based systems such as Amazon Alexa may be useful for collecting self-reported information in real time from participants of epidemiology studies using verbal input. In epidemiological research studies, self-reported data tend to be collected using short, infrequent questionnaires, in which the items require participants to select from predefined options, which may lead to errors in the information collected and lack of coverage. Voice-based systems give the potential to collect self-reported information “continuously” over several days or weeks. At present, to the best of our knowledge, voice-based systems have not been used or evaluated for collecting epidemiological data.

Objective
We aimed to demonstrate the technical feasibility of using Alexa to collect information from participants, investigate participant acceptability, and provide an initial evaluation of the validity of the collected data. We used food and drink information as an exemplar.

Methods
We recruited 45 staff members and students at the University of Bristol (United Kingdom). Participants were asked to tell Alexa what they ate or drank for 7 days and to also submit this information using a web-based form. Questionnaires asked for basic demographic information, about their experience during the study, and the acceptability of using Alexa.

Results
Of the 37 participants with valid data, most (n=30, 81%) were aged 20 to 39 years and 23 (62%) were female. Across 29 participants with Alexa and web entries corresponding to the same intake event, 60.1% (357/588) of Alexa entries contained the same food and drink information as the corresponding web entry. Most participants reported that Alexa interjected, and this was worse when entering the food and drink information (17/35, 49% of participants said this happened often; 1/35, 3% said this happened always) than when entering the event date and time (6/35, 17% of participants said this happened often; 1/35, 3% said this happened always). Most (28/35, 80%) said they would be happy to use a voice-controlled system for future research.

Conclusions
Although there were some issues interacting with the Alexa skill, largely because of its conversational nature and because Alexa interjected if there was a pause in speech, participants were mostly willing to participate in future research studies using Alexa. More studies are needed, especially to trial less conversational interfaces.
Challenges and strategies for gender mainstreaming policy in smart city development in Indonesia
Authors: Wahyudi Ruslan , Elanda Yelly 
Published: March 2023

A smart city is an urban policy innovation that can improve the community's quality of life by promoting environmental, social, and economic sustainability issues. The involvement and participation of civil society in developing smart cities is a major issue in implementing inclusive smart cities. However, gender issues are often forgotten in developing sustainable smart cities. This article examines two issues. First, the importance of gender issues integration in the development of sustainable smart cities in Indonesia. Second, the importance of gender mainstreaming policy in the development of smart cities in Indonesia. This is a desk study or literature study. Data were obtained from research results published in national and international journals. Data was obtained through accredited journal search engines, namely google scholar, SagePub and Researchgate. The research results show that development of smart city in Indonesia is not yet gender responsive, causing gender inequality gender and excluding women from development. To respond to this, thus there is a need for gender mainstreaming policies in developing smart cities through an overview of the challenges of gender mainstreaming in developing smart cities. Thus, this research recommends a gender mainstreaming strategy as a policy in developing smart cities in Indonesia. Gender mainstreaming policy in smart city development need to be to create a smart city life that is just, inclusive and involves women in development.
On Fibonacci Cordial Labeling of Some Snake Graphs
Authors: Sulayman Jolina E. , Pedrano Ariel C. 
Published: March 2023

Let an injective function f from vertex set V of a graph G to the set (F0, F1, F2, ..., Fn), where Fj is jth the Fibonacci number (j=0, 1, ..., n), is said to be Fibonacci cordial labeling if the induced function f* from the edge set E of graph G to the set {0,1} defined by f* (uv) = (f(u)+f(v)) (mod2) satisfies the condition |ef(0)- ef(1) ≤ 1, where ef(0) is the number of edges with label 0 and ef(1) is the number of edges with label 1. A graph which admits Fibonacci cordial labeling is called Fibonacci cordial graph.