bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the skin microbiota of healthy dogs and those with pyoderma
Authors: Stroich V. V. , Horiuk Y. V. 
Published: June 2023

Among the diseases of dogs, pyoderma is a frequent reason for referral to veterinary clinics. To effectively treat pyoderma and develop new strategies for preventing this disease, it is necessary to study the causes of formation and the conditions for the manifestation of pathogenic properties in causative agents. The purpose of the study will be to identify the microbiota isolated from the healthy skin of dogs and those with pyoderma. Washes were collected from the skin of various areas in clinically healthy dogs and dogs with pyoderma. The material was sown on selective media for the isolation of specific genera of microorganisms; after cultivation, pure cultures were identified according to the methods generally accepted in microbiological practice (cultural, tinctorial, morphological, and biochemical tests), according to Bergi's determinant. In addition, the following test systems were used: “STAPHY-test 16”, “En-coccus-test 36”, and “NEFERM test-24”. It was established that coagulase-negative and coagulase-positive species of staphylococci belong to the representatives of the autochthonous microbiota of the skin of clinically healthy dogs, which are isolated from different areas in 100 and 60–68.5 % of cases, respectively—the coagulase-positive species S. pseudintermedius and the coagulase-variable species S. schleiferi subsp. Coagulants occupy up to 65 % of the central niche, and СNS accounts for up to 30 % of all identified staphylococci. The causative agent of pyoderma in monoculture was S. pseudintermedius in 85.8 % of cases. In 57.1 % of cases, association with pyoderma was represented by СPS and Escherichia coli, and microbial association with СPS and Pseudomonas aeruginosa was detected 4.0 times less often – 14.3 % of cases. In 28.6 % of cases, microbial associations included СPS, Proteus in combination with intestinal or Pseudomonas aeruginosa. On the share of coagulase-positive staphylococci S. pseudintermedius, S. aureus, and coagulase-variable subspecies S. schleiferi subsp. Coagulants account for the central part of the staphylococcal microbiota (93.1 %), identified from inflammatory skin foci in pyoderma. At the same time, the species S. pseudintermedius significantly prevailed over other species; in particular, its share accounted for 78.4 % of the staphylococci isolated, which is 16.0 times more than S. aureus and 8.0 times more than S schleiferi subsp. coagulants So, in the etiology and pathogenesis of pyoderma in dogs, a significant role is played by СPS, in particular, the species S. pseudintermedius.
Components of physicochemical properties and chemical composition of mus-cle tissue of young pigs of different growth intensities, the level of their pheno-typic consolidation, and correlation
Authors: Khalak V. I. , Gutyj B. V. , Voloshchuk V. M. , Guta Z. A. , Verbelchuk T. V. , Ilchenko M. O. 
Published: June 2023

The work presents the results of the study of some biochemical indicators of blood serum, physicochemical properties, and chemical composition of muscle tissue of young pigs of the large white breed and the calculation of correlations between the main quantitative characteristics. The work was carried out in the agricultural formations of the Dnipropetrovsk region, the research center for biosafety and environmental control of agricultural resources of the Dnipro State Agrarian and Economic University, LLC “Globynskyi Myasokbinat” of the Poltava region, the zootechnical analysis laboratory of the Institute of Pig Breeding and Agro-industrial Production of the National Academy of Sciences and the animal husbandry laboratory of the state institution “Institute of Grain Crops of NAAS”. Studies of the fattening and meat qualities of young pigs of the large white breed show that the animals of the controlled population belong to the elite class by the age of reaching a live weight of 100 kg. This indicator in the animals of the experimental group ranges from 167 to 188 days. It was established that the number of samples of the longest back muscle of high quality according to the indicator “moisture retention capacity” is 12.0 %, “fat content” – 16.0%, “tenderness” – 12% and “color intensity” – 16%. A high level of phenotypic consolidation was established in the animals of the II experimental group (the age of reaching a live weight of 100 kg is 177–188 days) according to the content of total moisture (K1 = +0.458, K2 = +0.456), air-dry matter (K1 = +0.492, K2 = +0.500), ash (K1 = +0.527, K2 = +0.534), fat (K1 = +0.559, K2 = +0.518) and calcium (K1 = +0.385, K2 = +0.415). Reliable correlations were established between the following pairs of traits: moisture-holding capacity × protein content (r = -0.484, tr = 2.65), age at which live weight reached 100 kg × calcium (Ca) content (r = -0.392, tr = 2,15), age of reaching a live weight of 100 kg × moisture retention capacity (r = +0.447, tr = 2.40), age of reaching a live weight of 100 kg × loss during heat treatment (r = -0.390, tr = 2.14). This indicates the effectiveness of using the indicator “age of reaching 100 kg live weight, days” for early prediction of calcium (Ca), moisture retention capacity, and loss of muscle tissue during heat treatment in young pigs of the large white breed.
Energy Literacies
Authors: Schleser Max , Leontini Justin , Wheeler Melissa 
Published: June 2023

In October 2022, A/P Justin Leontini (Dept. of Mechanical and Product Design Engineering) and A/P Max Schleser (Dept. of Film, Games and Animation) ran a mobile storytelling workshop with a regional primary school in Victoria, Australia. Pupils learned digital storytelling and rookie smartphone filmmaking basics to engage in a discussion about energy. The co-created (Auguiste 2020) mobile stories are part of the development of an Energy Literacy Community Toolkit, which is developed by Schleser, Wheeler, Leontini and the Social Innovation Research Institute at Swinburne University of Technology for Central Victorian Greenhouse Alliance in connection with the Donald & Tarnagulla Microgrid Feasibility & Demonstration study. This study is supported by Centre for New Energy Technologies (C4NET) and the Department of Industry, Science, Energy and Resources (CVGA 2022) in Victoria, Australia.
This research creation project Where does Power come from? illustrates the Community Engagement: Donald and Tarnagulla Microgrid Feasibility Study project’s process and the co-created understanding for the next generation of power end-users. The research objective was to develop a community voice in the microgrid feasibility study. The co-created mobile stories are developed with an interest-based model (McCosker et al. 2018). Through understanding the communities’ perspectives and perceptions, this research worked with micro, horizontal hierarchies and ‘open space’ storytelling approaches (Zimmermann and De Michiel 2018, 2, 33 and 31). The theoretical underpinning draws upon a legacy of community arts (Dunn and Leeson 1997) and activism notion of ‘nothing about us without us’ (Charlton 2000). The co-created method enables to develop an understanding about particular thematic through applying a community point of view or language to develop a resource for the community.
Mobile story making (Schleser 2018) is a conversation starter. The pupil’s, their parents, neighbours and other community members will ‘like’ these videos because they are featured in these stories (Gondry 2008). The project shifts the conversation to the heart of the community and provides new community engagement touch points. Understanding local community needs such as reliability, cost and self-sufficiency is a key element in setting up a community microgrid.
Rural and regional communities tend to be at the end of the grid or have long distribution lines, leading to quality deterioration of the electricity supply. These communities and their businesses are seeking a balance of reliable, sustainable and low-cost electricity tailored to their needs (C4Net 2022).
Radical Restorative Justice of The People's CDC
Authors: Shafer Leah 
Published: June 2023

Four days before the 2022 State of the Union address, the CDC made a change to the risk-prevention pandemic map of the United States that changed the color of the map from red to green. Before the change, the CDC worked with guidelines that identified a substantially high risk as 50 cases per 100,000 people. After the change, the low risk category became 200 cases per 100,000 - a significant diminution of the calculation. As the People’s CDC notes, “The resulting shift from a red map to a green one reflected no real reduction in transmission risk. It was a resort to rhetoric: an effort to craft a success story that would explain away hundreds of thousands of preventable deaths and the continued threat the virus poses.”
The People’s CDC, a collaborative of volunteer health experts who offer policy recommendations and guidance around COVID-19 issues, reframes the official US responses to the global health crisis by disseminating critical information via social media. On their website People’s CDC identify their collaborative project: “working alongside community organizations, we are building collective power and centering equity as we work together to end the pandemic.”
In this presentation, I situate the equity-focused work of the People’s CDC within restorative justice and world building movements. Using their “layered, collective, and equitable” social media publications as a guide, I employ recent research on collective action to analyze the ways that the People’s CDC resists the hegemonic inequities embedded in state run public health systems and corporate mass media by redeploying and rewriting the rhetorical strategies of those systems. I argue that the People’s CDC’s ethos of care and collaboration is employing restorative world building practices as a response to global crisis. As they say, “We must urgently create a new normal in which commitment to community care drives policy and behavior. This charge requires sustained and collective action.”
Crafting Attention in Immersive and Interactive Practices
Authors: Çam Reşat Fuat , Behlil Melis 
Published: June 2023

This creation is part of the ongoing project “Positioning the Audience in Cinematic Virtual Reality”, supported by the Scientific and Technological Research Council of Turkey. The project engages with the attentional and corporeal resonance between spectators and visual experiences in participatory and immersive practices. In the continuing first phase of the project, we are designing and producing a VR creation that will then enable us to conduct audience research to focus on the attention practices of the viewers in the second phase. Hence, as a critical research-creation project it has two complementary and symmetrical foci that employ both theory and practice, and that are intertwined with one another. In tandem with making an interactive and photorealistic (cinematic) VR film where spectator crafts the very narrative unfolding by voluntary and involuntary attentional participation in a given scene, we are also developing a conceptual framework on how immersive and interactive technologies are conditioned by the freedom given to the spectator. As interdisciplinary project it brings together researchers from film studies, new media studies and psychology to develop a holistic understanding on the attention of spectator in moving image technologies.
In making, instead of using game-based interactivity technologies enabled by the controllers, the VR film uses gaze detection techniques and the attentional economy of the spectator as active defining element of the narrative unfolding. Combining both the bottom-up and the top-down attentional procedures it allows spectators to craft a circular experience and aims to problematize the craft of the spectator. In theory it aims to develop an historical and critical understanding of visual practices where spectator is the active producer of the optical experience in certain media ecology including technologies from proto-cinematic devices to the post-cinematic media constellation comprising video games, Virtual Reality etc. Therefore we aim to problematize and rend sensible the craft and the autonomy of the viewer by combining conventional academic research practices with artistic experimentation. The creation undertaken in the first phase of the research is aimed to result in multiple alternative designs, which will form the basis of our presentation at IFM2023.
Polyphonic Documentary
Authors: Odorico Stefano , Aston Judith 
Published: June 2023

This research creation presentation aims to showcase and stimulate discussion on our last round of creative practice interactive interventions developed within the ongoing collaborative and co creative project called: “Polyphonic Documentary” (www.polyphonicdocumentary.com).
Working as practice-based researchers and through creative practices, we argue that self-reflexivity is a key requirement for decolonizing the mind and the way in which we construct and tell stories (Aston, Odorico 2022). Our interest here is in considering how the interactive documentary form, through the use of interactive storytelling authoring tools, can facilitate this process.
Our project looks at the factual digital storytelling practice of interactive documentary through the lens of polyphony. We use the concept of polyphony as a research approach and method which encourages us to embrace complexity and plurality, and to respect different perspectives and points of view within a context of care.
El peritaje contable y la investigación fiscal por lavado de activos en el Perú
Authors: Ponce-Maluquish Luisa 
Published: June 2023

Objetivo: Determinar la relación del peritaje contable con la investigación fiscal por lavado de activos en el Perú.
Método: La investigación fue de tipo aplicada, no experimental, cuantitativa, correlacional y transversal. La muestra estuvo conformada por 63 profesionales de justicia (34 peritos contables y 29 fiscales (adjunto y titular). El procedimiento técnico de recojo fue la encuesta, con el cuestionario como instrumento. El análisis se desarrolló por medio del análisis descriptivo y bivariado, usando la prueba de Correlación de Spearman.
Resultados: Se evidenció que el peritaje contable tiene una relación significativa con la investigación fiscal por lavado de activos (rho=0.824; p=0.000). Además, las dimensiones identificación de hechos económicos para la obtención de juicios de valor (rho=0.617; p=0.000), control sobre el contenido del informe pericial (rho=0.704; p=0.000) y la valoración del informe pericial contable (rho=0.682; p=0.000) están relacionados significativamente con la investigación fiscal por lavado de activos. Sin embargo, la planificación del procedimiento del acto pericial contable (rho=0.221; p=0.082) no está relacionada significativamente con la investigación fiscal por lavado de activos.
Conclusión: El peritaje contable tiene una relación significativa fuerte y positiva en la investigación fiscal por lavado de activos en el Perú.
Biogenic polymer-based patches for congenital cardiac surgery: a feasibility study
Authors: Richert Emma , Nienhaus Andrea , Ekroll Jahren Silje , Gazdhar Amiq , Grab Maximilian , Hörer Jürgen , Carrel Thierry , Obrist Dominik , Heinisch Paul Philipp 
Published: June 2023

ObjectiveCurrently used patch materials in congenital cardiac surgery do not grow, renew, or remodel. Patch calcification occurs more rapidly in pediatric patients eventually leading to reoperations. Bacterial cellulose (BC) as a biogenic polymer offers high tensile strength, biocompatibility, and hemocompatibility. Thus, we further investigated the biomechanical properties of BC for use as patch material.MethodsThe BC-producing bacteria Acetobacter xylinum were cultured in different environments to investigate optimal culturing conditions. For mechanical characterization, an established method of inflation for biaxial testing was used. The applied static pressure and deflection height of the BC patch were measured. Furthermore, a displacement and strain distribution analysis was performed and compared to a standard xenograft pericardial patch.ResultsThe examination of the culturing conditions revealed that the BC became homogenous and stable when cultivated at 29°C, 60% oxygen concentration, and culturing medium exchange every third day for a total culturing period of 12 days. The estimated elastic modulus of the BC patches ranged from 200 to 530 MPa compared to 230 MPa for the pericardial patch. The strain distributions, calculated from preloaded (2 mmHg) to 80 mmHg inflation, show BC patch strains ranging between 0.6% and 4%, which was comparable to the pericardial patch. However, the pressure at rupture and peak deflection height varied greatly, ranging from 67 to around 200 mmHg and 0.96 to 5.28 mm, respectively. The same patch thickness does not automatically result in the same material properties indicating that the manufacturing conditions have a significant impact on durabilityConclusionsBC patches can achieve comparable results to pericardial patches in terms of strain behavior as well as in the maximum applied pressure that can be withstood without rupture. Bacterial cellulose patches could be a promising material worth further research.
Physical and chemical characteristics of active sulfur flows observed at Lastarria volcano (northern Chile) in January 2019
Authors: Inostroza Manuel , Fernandez Bárbara , Aguilera Felipe , Layana Susana , Walter Thomas R. , Zimmer Martin , Rodríguez-Díaz Augusto , Oelze Marcus 
Published: June 2023

Molten sulfur is found in various subaerial volcanoes. However, limited records of the pools and flows of molten sulfur have been reported: therefore, questions remain regarding the physicochemical processes behind this phenomenon. A suite of new sulfur flows, some of which active, was identified at the Lastarria volcano (northern Chile) and studied using satellite imagery, in situ probing, and temperature and video recording. This finding provides a unique opportunity to better understand the emplacement mechanisms and mineral and chemical compositions of molten sulfur, in addition to gaining insight into its origin. Molten sulfur presented temperatures of 124–158°C, with the most prolonged sulfur flow reaching 12 m from the source. Photogrammetric tools permitted the identification of levees and channel structures, with an estimated average flow speed of 0.069 m/s. Field measurements yielded a total volume of 1.45 ± 0.29 m3 of sulfur (equivalent to ∼2.07 tons) mobilized during the January 2019 event for at least 408 min. Solidified sulfur was composed of native sulfur with minor galena and arsenic- and iodine-bearing minerals. Trace element analysis indicated substantial enrichment of Bi, Sb, Sn, Cd, as well as a very high concentration of As (&gt;40.000 ppm). The January 2019 molten sulfur manifestations in Lastarria appear to be more enriched in As compared to the worldwide known volcanoes with molten sulfur records, such as the Shiretoko-Iozan and Poás volcanoes. Furthermore, their rheological properties suggest that the “time of activity” in events such as this could be underestimated as flows in Lastarria have moved significantly slower than previously thought. The origin of molten sulfur is ascribed to the favorable S-rich chemistry of fumarolic gases and changes in host rock permeability (fracture opening). Molten sulfur in Lastarria correlates with a peak in activity characterized by high emissions of SO2 and other acid species, such as HF and HCl, in addition to ground deformation. Consequently, molten sulfur was framed within a period of volcanic unrest in Lastarria, triggered by changes in the magmatic-hydrothermal system. The appearance of molten sulfur is related to physicochemical perturbations inside the volcanic system and is perhaps a precursor of eruptive activity, as observed in the Poás and Turrialba volcanoes.
A novel frameshift mutation in DNAH6 associated with male infertility and asthenoteratozoospermia
Authors: Huang Fei , Zeng Jun , Liu Dan , Zhang Jing , Liang Boluo , Gao Jingping , Yan Rong , Shi Xiaobo , Chen Jianlin , Song Wanjuan , Huang Hua-Lin 
Published: June 2023

IntroductionAsthenoteratozoospermia is one of the most common causes of male infertility. Several genes have been identified as genetic causative factors, but there is a considerable genetic heterogeneity underlying asthenoteratozoospermia. In this study, we performed a genetic analysis of two brothers from a consanguineous Uighur family in China to identify gene mutations causative for asthenoteratozoospermia-related male infertility.MethodsTwo related patients with asthenoteratozoospermia from a large consanguineous family were sequenced by whole-exome sequencing and Sanger sequencing to identify disease-causing genes. Scanning and transmission electron microscopy analysis revealed ultrastructural abnormalities of spermatozoa. Quantitative real-time PCR (qRT-PCR) analysis and immunofluorescence (IF) analysis were used to assess the expression of the mutant messenger RNA (mRNA) and protein.ResultsA novel homozygous frameshift mutation (c.2823dupT, p.Val942Cysfs*21) in DNAH6 was identified in both affected individuals and was predicted to be pathogenic. Papanicolaou staining and electron microscopy revealed multiple morphological and ultrastructural abnormalities of affected spermatozoa. qRT-PCR and IF analysis showed abnormal expression of DNAH6 in affected sperm, probably due to premature termination code and decay of abnormal 3′ untranslated region (UTR) region of mRNA. Furthermore, intracytoplasmic sperm injection could achieve successful fertilization in infertile men with DNAH6 mutations.DiscussionThe novel frameshift mutation identified in DNAH6 may contribute to asthenoteratozoospermia. These findings expand the spectrum of genetic mutations and phenotypes associated with asthenoteratozoospermia and may be useful for genetic and reproductive counseling in male infertility.