bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated DNA Methylation/RNA Profiling in Middle Temporal Gyrus of Alzheimer’s Disease
Authors: Piras Ignazio S. , Brokaw Danielle , Kong Yinfei , Weisenberger Daniel J. , Krate Jonida , Delvaux Elaine , Mahurkar Swapna , Blattler Adam , Siegmund Kimberly D. , Sue Lucia , Serrano Geidy E. , Beach Thomas G. , Laird Peter W. , Huentelman Matthew J. , Coleman Paul D. 
Published: January 2023

AbstractAlzheimer’s disease is a neurodegenerative disorder clinically defined by gradual cognitive impairment and alteration in executive function. We conducted an epigenome-wide association study (EWAS) of a clinically and neuropathologically characterized cohort of 296 brains, including Alzheimer’s disease (AD) and non-demented controls (ND), exploring the relationship with the RNA expression from matched donors. We detected 5246 CpGs and 832 regions differentially methylated, finding overlap with previous EWAS but also new associations. CpGs previously identified in ANK1, MYOC, and RHBDF2 were differentially methylated, and one of our top hits (GPR56) was not previously detected. ANK1 was differentially methylated at the region level, along with APOE and RHBDF2. Only a small number of genes showed a correlation between DNA methylation and RNA expression statistically significant. Multiblock partial least-squares discriminant analysis showed several CpG sites and RNAs discriminating AD and ND (AUC = 0.908) and strongly correlated with each other. Furthermore, the CpG site cg25038311 was negatively correlated with the expression of 22 genes. Finally, with the functional epigenetic module analysis, we identified a protein–protein network characterized by inverse RNA/DNA methylation correlation and enriched for “Regulation of insulin-like growth factor transport”, with IGF1 as the hub gene. Our results confirm and extend the previous EWAS, providing new information about a brain region not previously explored in AD DNA methylation studies. The relationship between DNA methylation and gene expression is not significant for most of the genes in our sample, consistently with the complexities in the gene expression regulation.
Graphical Abstract
A novel technology of solarization and phytoremediation enhanced with biosurfactant for the sustainable treatment of PAH-contaminated soil
Authors: Futughe Anthony Esimajemite , Jones Huw , Purchase Diane 
Published: January 2023

AbstractHydrocarbon-contaminated land has been a significant issue throughout Nigeria’s Niger Delta since the discovery of crude oil in 1956. This paper proposes a novel and sustainable technique involving soil solarization, phytoremediation and biosurfactant to treat polycyclic aromatic hydrocarbon (PAH) contamination. The treatment effect on PAH reduction, plant growth, rhizosphere microorganisms and their enzymatic activities was evaluated. Twenty-eight days of solarization was carried out before the introduction ofChromolaena odorataseedlings for an 84-day phytoremediation period using a 4 × 4 (vegetated) and 2 × 4 (non-vegetated) cell microcosms to simulate the Niger Delta’s subtropical conditions. Soil solarization resulted in significant PAH reduction (p ≤ 0.01) of phenanthrene, fluoranthene and benzo(a)pyrene with means reduction of 60.0%, 38.7% and 36.1% compared to their non-solarized counterparts with 18.0%, 18.0% and 18.8% at 95% CI (32.7, 51.3), (15.4, 26.1) and (8.0, 26.6), respectively. In post-solarization, all solarized and vegetated treatment groups significantly reduced (p ≤ 0.01) PAHs compared to their respective counterparts, while biosurfactant contribution in this combination was negligible (p ≥ 0.05). The growth parameters ofC. odorata,total soil/rhizosphere heterotrophic microorganisms and their enzymatic activities of dehydrogenase and urease increased in all solarized treatments indicating essential biochemical processes. The novel and successful integration of soil solarization and phytoremediation using indigenousC. odorataas a combined technique to treat even the most recalcitrant form of hydrocarbons (PAHs) brings up new opportunities for a sustainable method of cleaning up contaminated land in Nigeria's oil-rich Niger Delta.Graphical Abstract
The vocal apparatus: An understudied tool to reconstruct the evolutionary history of echolocation in bats?
Authors: Brualla Nicolas L. M. , Wilson Laura A. B. , Doube Michael , Carter Richard T. , McElligott Alan G. , Koyabu Daisuke 
Published: January 2023

AbstractLaryngeal echolocation in bats could have evolved following two scenarios: a single origin from a common ancestor or an independent acquisition inside the two clades Yinpterochiroptera and Yangochiroptera. Later, some members of Yinpterochiroptera possibly lost their ability to echolocate. In bats, the larynx produces vocalizations for communication and, in most species, for echolocation. Here, we describe how comparative chiropteran laryngeal morphology is a novel area of research that could improve the understanding of echolocation and may help resolve the evolutionary history of bats. This review provides morphological descriptions and comparisons of the bat larynx and bioacoustics interpretations. We discuss the importance of understanding: (1) laryngeal sound production so it may be linked with the evolution of the chiropteran auditory system; and (2) the evolution of laryngeal morphology to understand the ecological and behavioural aspects of bat biology. We find that a strong phylogenetic signal is potentially the main source explaining macroevolutionary variation in laryngeal form among bats. We predict that the three parameters of sound production in echolocation (frequency, intensity, and rate of calls) are independently modulated by different laryngeal components, but this hypothesis remains understudied in terms of species diversity.
Ionic Liquid/ZnO Assisted Preparation of High Barrier Cellulose Nanocomposite Films by In Situ Ring-Opening Polymerization of Lactide Monomers
Authors: Amini Elahe , Valls Cristina , Yousefi Hossein , Roncero M. Blanca 
Published: January 2023

AbstractCellulose-based composites have aroused increasing interest as potential replacements for fossil fuel-based plastics. In this work, transparent cellulose-grafted-PLA nanocomposite films were prepared by grafting polylactide (PLA) onto cellulose. PLA was synthesized by in situ ring opening polymerization from a regenerated cellulose matrix, using zinc oxide nanoparticles (ZnONPs) in 1-ethyl-3-methylimidazolium acetate (EmimAc) as solvent. A facile route was devised to modify the starting material (cellulose paper) by partial dissolution and regeneration that used an ionic liquid (IL) as a smart nanowelding agent to assemble nanometric cellulose structures. The influence of the proportion of ZnONPs (1–5 wt%) and $$\textit{L}$$
L
-lactide (LA) monomers (10–70 wt%) used on the properties of the resulting nanocomposite films was examined by comparison with an all-cellulose composite (ACC) and pure cellulose paper. Incorporating ZnONPs and PLA was found to enhance the mechanical, barrier and optical properties of the films. The maximum tensile strength and best barrier properties were those of a film obtained from 5%ZnONPs and 70%LA. FTIR spectra confirmed a new form of interaction between PLA and the regenerated cellulose matrix. Also, XRD spectra revealed a transition from cellulose I to II and an increase in the proportion of noncrystalline cellulose through partial dissolution and regeneration. Although the surface morphology of the nanocomposite films was influenced by the presence of ZnONPs and PLA chains, their color and chemical structure were not. The transparent cellulose-grafted-PLA nanocomposite films obtained are highly promising as packaging materials.
Turning to Religion During COVID-19 (Part I): A Systematic Review, Meta-analysis and Meta-regression of Studies on the Relationship Between Religious Coping and Mental Health Throughout COVID-19
Authors: Pankowski Daniel , Wytrychiewicz-Pankowska Kinga 
Published: January 2023

AbstractThe COVID-19 pandemic and the many associated socio-economic changes constitute a stressful event that required adaptation to new, dynamic, and often threatening conditions. According to the literature, coping strategies are one of the factors that determine a person’s degree of adaptation to stressful situations. A systematic review and meta-analysis was performed on the relationship between religious coping and selected indicators of mental health. Due to the large amount of data, this work has been divided into two parts: this first part discusses positive mental health indicators, while the second discusses negative mental health indicators (Pankowski & Wytrychiewicz-Pankowska, 2023). A systematic review of PubMed, Science Direct, the Cochrane Library, Google Scholar, the Database of Abstracts of Reviews of Effects, and Google Scholar databases was carried out. In addition to the synthesis of information obtained from the research, a meta-analysis of correlation was also performed to determine the strengths of the relationships between the analysed variables, and selected moderators were assessed using meta-regression. Quality of life, well-being, satisfaction with life, happiness, and post-traumatic growth were the positive mental health indicators considered. Meta-analyses indicated a statistically significant relationship between positive religious coping and flourishing (well-being) with overall correlation values of 0.35 [0.30; 0.40]. Further calculations also indicated a relationship between negative religious coping and flourishing − 0.25 [− 0.34; − 0.15]. Data synthesis shows associations between religious coping and such indicators as satisfaction with life and post-traumatic growth, but these issues require further investigation.
The GROWBABY Research Network: A Framework for Advancing Health Equity Through Community Engaged Practice-Based Research
Authors: Viglione Clare , Boynton-Jarrett Renée 
Published: January 2023

Abstract
Purpose
Preventive health care, delivered through well child care visits, serves as a universal and primary entry point for promoting child wellbeing, yet children with lower socioeconomic status and children of color receive less consistent and lower quality preventive health care. Currently, limited research exists comparing models for delivering preventive care to children and their impact on longstanding racial/ethnic and socioeconomic inequities.

Description
Practice-based research networks can help to advance health equity by more rapidly studying and scaling innovative, local models of care to reduce racial/ethnic and socioeconomic inequities in primary care and preventive care utilization. This paper outlines a framework of community engagement that can be utilized by practice-based research networks to advance health equity and details the application of the framework using the GROWBABY Research Network (GROup Wellness Visits for BABies and FamilY Research Network).

Assessment
The GROWBABY Research Network launched in 2020, engaged clinical practices utilizing this unique model of group well childcare - CenteringParenting® - with the following goals: to promote collaboration among researchers, clinicians, patients, and community members; facilitate practice-based research; and increase the use of shared assessment measures and protocols. As a research collaborative, the GROWBABY Research Network connects clinical partners facing similar challenges and creates opportunities to draw upon the assets and strengths of the collective to identify solutions to the barriers to research participation.

Conclusion
Primary care, practice-based research networks like the GROWBABY Research Network that intentionally integrate community engagement principles and community-based participatory research methods can advance equitable health care systems and improve child wellbeing.
The influence of a trap state on the photoluminescence decay times under single pulse excitation
Authors: Chiu K. P. 
Published: January 2023

AbstractWe numerically calculated the time-resolved photoluminescence spectra using the bimolecular trapping-detrapping model. The variations of carrier lifetimes are investigated by changing the carrier recombination and trapping rate constants, as well as the concentration of available trapping states.
Welfare comparisons within and across species
Authors: Browning Heather 
Published: January 2023

AbstractOne of the biggest problems in applications of animal welfare science is our ability to make comparisons between different individuals, both within and across species. Although welfare science provides methods for measuring the welfare of individual animals, there’s no established method for comparing measures between individuals. In this paper I diagnose this problem as one of underdetermination—there are multiple conclusions given the data, arising from two sources of variation that we cannot distinguish: variation in the underlying target variable (welfare experience) and in the relationship of measured indicators to the target. I then describe some of the possible methods of making comparisons, based on the use of similarity assumptions that will have greater or lesser justification in different circumstances, and the alternative methods we may use when direct comparisons are not possible. In the end, all our available options for making welfare comparisons are imperfect, and we need to make explicit context-specific decisions about which will be best for the task at hand while acknowledging their potential limitations. Future developments in our understanding of the biology of sentience will help strengthen our methods of making comparisons.
Spleen stiffness: a predictive factor of dismal prognosis in liver cirrhosis
Authors: Karagiannakis Dimitrios S. , Stefanaki Katerina 
Published: January 2023

Abstract
Portal hypertension (PH) is a major complication of liver cirrhosis, as it predisposes to the development of serious clinical manifestations such as ascites, hepatic encephalopathy and variceal bleeding, aggravating the prognosis of patients. Hepatic vein pressure gradient (HVPG) is considered the reference method for the estimation of the presence and severity of PH, but this procedure is available only in specialized centers. Alternatively, many non-invasive methods have been proposed in order to substitute HVPG. Among them, liver stiffness measurement (LSM) has been widely used, as it has been shown to correlate well with HVPG, though this relationship seems to weaken in values of HVPG higher than 12 mmHg, the threshold of serious complications development. Several studies supported the use of spleen stiffness measurement (SSM) instead of LSM, anticipating to a more adequate assessment of this advanced stage of PH. The aim of this paper is to critically appraise and summarize the literature about the role of SSM as a predictive tool of liver decompensation and prognosis, highlighting the strengths and the potential limitations of the studies published so far.

Expert’s opinion
The utility of SSM in ruling out high risk for bleeding varices in cirrhotic patients has been demonstrated, driving the Baveno VII consensus to encompass SSM in its last recommendations, though its use in patients with non-viral cirrhosis remains to be validated. We believe that in the near future, SSM alone or combined with other tests, will being used not only for sparing upper endoscopies, but also for predicting decompensation and prognosis in advanced compensated cirrhotic patients, regardless of liver disease’s etiology. Herein, we present the data that support this consideration, pointing out these issues that should further be investigated in order to elucidate and intensify the value of SSM in the management of patients with liver cirrhosis.
The Biopolitics of Education in the Third Reich's ‘Special Schools’ and ‘Elite Schools’
Authors: Roche Helen , Pine Lisa 
Published: January 2023

AbstractWhile discussion of eugenics and biopolitics during the Third Reich has largely focused upon the regime's most destructive and genocidal policies, this article concentrates on Nazi ‘special schools’ and ‘elite schools’ as a crucial sphere of quasi-eugenic thought and praxis, drawing attention to education as a previously under-researched category of intervention in the history of modern biopolitics. The article also sheds new light on the racialized nature of the Nazi ‘national community’ (the Volksgemeinschaft), and contributes to recent debates on the Third Reich's status as a ‘racial state’ which suggest that the National Socialist regime was driven less by fanatical adherence to racial ideology, and more by a mixture of anthropological and eugenic racism, combined with productivist pragmatism. The two case-studies draw attention to less familiar corners of the National Socialist pedagogical landscape, covering both extremes of the spectrum of biological selection in education, from the negative, eugenic policies applied to supposedly ‘abnormal’ pupils at the so-called ‘special schools’ (Hilfsschulen), to the ‘positive’ biological selection of elite-school applicants at the National Political Education Institutes (Nationalpolitische Erziehungsanstalten, NPEA), the regime's principal training institutions for the future elite of the Third Reich.
Welfare assessment of broilers through examination of haematomas, foot-pad dermatitis, scratches and breast blisters at processing
Authors: Gouveia KG , Vaz-Pires P , da Costa P Martins 
Published: January 2023

AbstractAnalysis of post mortem lesions is a common means for assessing poultry welfare during rearing and pre-slaughter handling. The aim of this study was to determine the influence of: age, sex, rearing system, total number of birds on-farm, period between catch and slaughter and distance from poultry house to processing plant on the incidence of: foot-pad dermatitis (FPD), breast blisters (BB), bruises and scratches. Rearing systems consisted of extensive indoor (EI) and traditional free-range (TFR). Accordingly, birds were reared under non-intensive conditions (< 12 broilers m−2). In TFR, the maximum flock size was 4,800, birds had continuous daytime access to open-air runs from at least the age of six weeks, and the minimum slaughter age was 81 days; whereas EI rearing was exclusively indoor and birds were slaughtered at 56 days or later. Observations occurred daily after plucking and each bird was examined for: haematomas, scratches, FPD and BB. Logistic regressions were applied to study the relationship between variables. Farms holding between 10,000-20,000 birds were the most affected with FPD, BB and scratches. The oldest birds (91-100 days) showed the greatest incidence of FPD and scratches. TFR birds exhibited the highest prevalence of bruises and lowest of FPD. BB were most common among birds travelling between 31-60 km from poultry farm to processing plant while scratches were more frequent at greater distances (91-120 km). Male broilers were more predisposed to BB than females. Scratching increased relative to the number of hours that birds awaited slaughter. Despite the fact that only a relatively small number of broiler flocks were examined, these findings may have implications for the draft of future European legislation regarding poultry welfare.
Inter-observer and test-retest reliability of on-farm behavioural observations in veal calves
Authors: Bokkers EAM , Leruste H , Heutinck LFM , Wolthuis-Fillerup M , van der Werf JTN , Lensink BJ , van Reenen CG 
Published: January 2023

AbstractThe objective of this study was to investigate inter-observer and test-retest reliability of different behavioural observations to be used in an on-farm, animal welfare monitoring system for veal calves. Twenty-three veal calf farms, varying in size, housing system, feeding regime and age of the calves were visited twice with two observers, simultaneously. Behavioural tests were conducted in eight pens per farm, measuring the response of calves to: a human entering the barn; a novel object; a passive, unfamiliar person; disturbance in the pen and an active approach by an unfamiliar and a familiar person. Furthermore, behaviour was recorded 20 min before and 20 min after feeding in eight other pens per farm. For all behavioural tests, inter-observer reliability was very high. Farm effects and test-retest reliabilities were high and significant for all behavioural tests, except for the test measuring response to disturbance in the pen. Although the active approach test with the familiar person was reliable, it was not feasible in practice due to the availability of the farmer. Since the active approach test with the unfamiliar person gave similar results, this test was recommended for an on-farm animal welfare monitoring system. For most behavioural elements recorded around feeding, farms differed significantly and interobserver and test-retest reliabilities were high as well as being significant. The behavioural tests with entering the barn, novel object and unfamiliar person, and the behavioural observations before and after feeding were feasible and distinctive and reliable enough to be performed on-farm. These methods are promising tools to use as a monitor of animal welfare in veal calves.
Peripartum health and welfare of Holstein-Friesian cows in a confinement-TMR system compared to a pasture-based system
Authors: Olmos G , Mee JF , Hanlon A , Patton J , Murphy JJ , Boyle L 
Published: January 2023

AbstractThe greatest challenge to the welfare of dairy cows occurs in the peripartum period. Given the perception that cow welfare is better in more natural environments, it was hypothesised that cows in a PASTURE-based production system (cubicle housing with grass silage pre-partum and rotational grazing with concentrate supplementation post-partum) would have improved peripartal welfare compared to cows in a HOUSED production system (cubicle housing with a total mixed ration [TMR], pre-partum and post-partum). Blood samples were analysed for acute phase proteins (APP), cortisol, white blood cell (WBC) differential and counts and other biochemical metabolites as non-specific indicators of sub-clinical ill-health and nutritional stress. Daily monitoring of rectal temperature (RT) and rumen fill (RF) scores were used to monitor ill-health and nutritional status. Reproductive health and welfare (calving difficulty, retained placenta, puerperal metritis, endometritis and oestrous cyclicity) was also recorded. No differences were found between treatments for APP, cortisol or WBC. Blood metabolite differences indicated that PASTURE cows were under greater nutritional stress than HOUSED cows. HOUSED cows showed an increase in RF score from day 0 to 10 post-partum and had a higher RF score than PASTURE cows. PASTURE cows had an overall lower RT and lower incidence of reproductive disorders. Results primarily reflect nutritional differences between treatments with PASTURE cows showing greater potential nutritional/metabolic stress in early lactation which has attendant implications for welfare. Nevertheless, this did not result in inferior health and, in accordance with our hypothesis, PASTURE cows’ reproductive health and welfare tended to be better than that of HOUSED cows.