bluuebunny/crossref_metadata_2025_split huggingface dataset:
The predictive significance of chromobox family members in prostate cancer in humans
Authors: Xu Xiaoting , Lai Cong , Luo Jiawen , Shi Juanyi , Guo Kaixuan , Hu Jintao , Mulati Yelisudan , Xiao Yunfei , Kong Degeng , Liu Cheng , Huang Jingang , Xu Kewei 
Published: September 2023

Abstract
Purpose The Chromobox (CBX) family proteins are crucial elements of the epigenetic regulatory machinery and play a significant role in the development and advancement of cancer. Nevertheless, there is limited understanding regarding the role of CBXs in development or progression of prostate cancer (PCa). Our objective is to develop a unique prognostic model associated with CBXs to improve the accuracy of predicting outcomes of patients with PCa.
Methods Transcriptome sequencing and clinical data for PCa were obtained from the Cancer Genome Atlas and Gene Expression Omnibus databases. The data was then analyzed to identify differential expression, assess prognostic value, determine gene pathway enrichment, and evaluate immune cell infiltration. COX regression analysis was utilized to identify the independent prognostic factors that impact disease-free survival (DFS) in PCa, and subsequently, a nomogram was created. In vitro proliferation, migration and invasion assay were conducted to examine the function of CBX2 in PCa.
Results CBX2, CBX3, CBX4, and CBX8 were upregulated, whereas CBX6 and CBX7 were downregulated in PCa tumor tissues. The expression level of these genes differs depending on the cancer's stage and grade. A negative outcome is associated with patients who have elevated levels of CBX1, CBX2, CBX3, CBX4 and CBX8 expression. An independent prognostic factors for PCa were the expression level of CBX2 and T stage, as well as Gleason score, as determined by Cox regression analysis. Additionally, a nomogram was created. The infiltration level of various immune cells is associated with the expression level of CBX2. In vitro studies have shown that the knockdown of CBX2 can greatly impede the growth, migration and invasion of PCa cells.
Conclusion CBX2 is involved in the development and advancement of PCa, suggesting its potential as a reliable prognostic indicator for PCa patients.
ZNF251 is a novel prognostic biomarker and correlated with the immunosuppressive microenvironment in hepatocellular carcinoma
Authors: Zhang Zeyu , Chen Yuxin , Xie Tan , He Shiqing , Wei Xin , Wang Gang , Liu Bin , Sun Nan , Wang Feitong 
Published: September 2023

Abstract
Background
Hepatocellular carcinoma (HCC) persists as the most common malignant tumor worldwide. Therefore, it is crucial to investigate its pathogenic mechanism and explore its potential prognostic and therapeutic targets However, the role of zinc finger protein (ZNF)251 in HCC is unclear.
Methods
The Cancer Genome Atlas (TCGA)-HCC transcriptional profiles were used to investigate the ZNF251 expression in HCC and normal samples and explore the effect of ZNF251 on HCC prognosis. ZNF251 expression and its role in pan-cancer were examined via several other databases ZNF251 mRNA and protein expression levels in clinical HCC tissues and cell lines were measured by RT-qPCR and western blotting, respectively. Following small interfering RNA (siRNA)-mediated ZNF251 knockdown, the effects of ZNF251 on HCC cell proliferation, migration, and invasion were evaluated in vitro experiments. The effect of ZNF251 on HCC growth in vivo was investigated via a nude mouse subcutaneous model. Furthermore, the effect of ZNF251 on infiltrating immune cells and the associations between ZNF251 and the genes of interest were analyzed with TIMER2 and GEPIA2. Finally, the relationship between ZNF251 and chemokines was investigated via TISIDB.
Results
ZNF251 was highly expressed in HCC, and promoted HCC cell proliferation, migration, and invasion by activating the extracellular-regulated protein kinase (ERK) signaling pathway. ZNF251 was associated with poor prognosis of HCC and was highly expressed in most cancers. Further analyses revealed that ZNF251 was positively associated with regulatory T cell (Treg) infiltration and Treg-related immunosuppressive cytokine expression. Moreover, ZNF251 was tightly related to the expression of classic exhaustion markers of CD8+ T cells. ZNF251 significantly affected the expression of 10 chemokines in HCC (CCL2, CCL4, CCL5, CCL11, CCL13, CCL14, CCL23, XCL2, CXCL17, CCL20), which indicated that ZNF251 might affect HCC immune profiles by regulating chemokine expression.
Conclusion
ZNF251 functions as an oncogene in HCC and can serve as a new prognostic biomarker and therapeutic target.
Aesthetic Subunit Reconstruction of Basal Cell Carcinoma Face
Authors: Garg Paheli , Jain Rakesh , Vashistha Aakansha , Khushalani Asha 
Published: September 2023

Abstract
Background
Arising from the basal cell layer of epidermis; BCC is one of the most common cutaneous malignancy encountered in elderly (77%) followed by SCC(20%) and malignant melanoma(3%).
Material and method
: A prospective observational study was conducted at Department of Plastic and Reconstructive Surgery with 50 patients included for over a span of 2 years. Patients with biopsy confirmed BCC lesion over face were included and were grouped on the basis of subunit involved and type of flap done.
Results
50 patients were included which comprised of 16(32%) males and 34(68%) females. Mean age was 61+/-10.2 years. Smallest defect size was of 1.5 cm2 whereas largest defect size was of 5 cm2. Cheek was the most common site of occurrence. Cheek advancement flap was the most commonly performed flap.
Conclusion
Reconstruction of facial subunit post BCC excision with local tissue has superior aesthetic outcome in terms of colour and texture than any other modality.
Temporal dynamics and genomic programming of plasma cell fates
Authors: Singh Harinder , Vijay Godhev Kumar Manakkat , Xu Heping 
Published: September 2023

Abstract
Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Using a model antigen, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using scRNA-seq+BCR-seq in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveal a novel PC transition state that gives rise to functionally competent PC precursors. The latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived PCs, based on extended antigen encounters followed by reduced antigen availability.
The Effect of Perioperative Lidocaine During Modified Radical Mastectomy on Postoperative Pain and Immune Response. A randomized clinical trial
Authors: Hadavi SeyedMohammad Reza , Sahmeddini Mohammad Ali , Khademi Saeed , Boostani Nazila 
Published: September 2023

Abstract
Introduction:
Breast cancer is the most common cancer among women. Surgical resection of the breast mass could induce an inflammatory response, which increases cytokines such as interleukin IL beta-1 and IL-6. These mediators lead to peripheral and central sensitization and induce hyperalgesia. In this study, we hypothesized that perioperative lidocaine infusion could not only reduce serum interleukin levels but also reduce postoperative pain severity.
Patients and methods:
In this randomized clinical trial, 90 women who were candidates for elective mastectomy under general anesthesia were enrolled and randomly allocated into two groups. In group A, patients received 2 mg/kg of lidocaine stat and 1.5 mg/Kg/hr of lidocaine infusion perioperatively, and patients in group B received normal saline. Serum levels of interleukin 1 beta and 6 were measured before the start of infusions and 24 hours after. Also, postoperative pain was evaluated until 24 hours postoperatively.
Results
The mean visual analog post-operation was significantly lower in the lidocaine group compared to the control group (P < 0.001). The baseline mean serum levels of interleukin 1 beta and 6 in pre-operative time in both groups were not different (P > 0.05). However, 24 hours postoperatively, the mean serum level of interleukin 6 was higher in the control group compared to the lidocaine group (P 0.001).
Conclusion
Perioperative systemic lidocaine infusion in patients with breast cancer could modulate the production of IL-6, and through the reduction of this cytokine, patients experienced better pain control in the immediate postoperative period.
Content validity and reliability of the Exploring EXPRESSions of Autism through Body Language (EXPRESS) tool
Authors: Ferreira Ilana D , Arbour-Nicitopoulos Kelly P , Brian Jessica , Wright F Virginia 
Published: September 2023

Abstract
Children with autism spectrum disorder (ASD) participate in less physical activity than the recommended physical activity guidelines. This may be attributable, in part, to community program instructors’ limited knowledge of ASD, specifically, their awareness of the nonverbal expression differences and consequent challenges with understanding these children’s experiences and reactions during program activities. We developed the Exploring EXPRESSions of Autism through Body Language (EXPRESS) observational rating tool to increase awareness of body language communication of children with ASD, and through its use, hopefully enhance the relationship between instructors and children with ASD within community physical activity programs. The purpose of this study was to assess two key psychometric aspects of the 12-item EXPRESS-Code. Three parents of children with ASD participated in interviews to evaluate content validity related to item interpretation (positive/negative body language cue categories). Inter-rater reliability was assessed by having two raters use the EXPRESS-Code to score videos of 26 children with ASD (6–12 years) performing an advanced gross motor assessment. The EXPRESS-Code met the target for content validity with 88.5% agreement on item categorization, although parents recommended renaming the body language categories as ‘engaged’ and ‘not engaged’. Intra- and inter-rater reliability estimates were excellent for the ‘engaged’/ ‘not engaged’ cue categories (ICCs 0.95–0.97). Next steps for the EXPRESS-Code include assessment of the impact of use on: 1) the relationship of a child with ASD and their instructor, 2) instructors’ confidence working with children with ASD, and 3) engagement and enjoyment of the child with ASD within a physical activity program.
Genomic Classifiers and Prognosis of Localized Prostate Cancer: A Systematic Review
Authors: Boyer Matthew , Carpenter David , Gingrich Jeffrey , Raman Sudha , Sirohi Deepika , Tabriz Amir Alishahi , Rompre-Brodeur Alexis , Lunyera Joseph , Basher Fahmin , Bitting Rhonda , Kosinski Andrezj , Cantrell Sarah , Gordon Adelaide , Ear Belinda , Gierisch Jennifer , Jacobs Morgan , Goldstein Karen 
Published: September 2023

Abstract
Background
Refinement of the risk classification for localized prostate cancer is warranted to aid in clinical decision making. A systematic analysis was undertaken to evaluate the prognostic ability of three genomic classifiers, Decipher, GPS, and Prolaris, for biochemical recurrence, development of metastases and prostate cancer specific mortality in patients with localized prostate cancer.
Methods
Data Sources: MEDLINE, Embase, and Web of Science were queried for reports published January 2010 to April 2022.
Study Selection: Prospective or retrospective studies reporting prognosis for patients with localized prostate cancer.
Data Extraction: Relevant data were extracted into a customized database by 1 researcher with a second over reading. Risk of bias was assessed using a validated tool for prognostic studies, Quality in Prognosis Studies (QUIPS). Disagreements were resolved by consensus or by input from a third reviewer. We assessed certainty of evidence by GRADE incorporating adaptation for prognostic studies.
Results
Data Synthesis: A total of 39 studies (37 retrospective) involving over 10 000 patients were identified. Twenty-two assessed Decipher, 5 GPS, and 14 Prolaris. Thirty-four studies included patients who underwent prostatectomy. Based on very low to low certainty of evidence, each of three genomic classifiers modestly improved upon the prognostic ability for biochemical recurrence, development of metastases, and prostate cancer specific mortality compared to standard clinical risk classification schemes
Limitations: Downgrading of confidence in the evidence stemmed largely from bias due to the retrospective nature of the studies, heterogeneity in treatment received, and era in which patients were treated (i.e., prior to 2000s).
Conclusions:
Genomic classifiers provide a small but consistent improvement upon the prognostic ability of clinical classification schemes which may be helpful when treatment decisions are uncertain. However, definitive evidence from current management-era data is needed.
An updated review of synthesis and characterization of some new thiazolidinones containing coumarin moiety and their antimicrobial study.
Authors: Kushwaha Shekhar Prakash 
Published: September 2023

Abstract
Purpose: The main purposes of this study were to investigate the therapeutic qualities of small ring heterocycles containing nitrogen and sulfur, with a focus on thiazoles, and their potential as pharmaceutically significant compounds. The research aimed to explore the biological actions of substances with thiazole nuclei, including anti-inflammatory, antibacterial, and antifungal characteristics.
Methods: First, coumarin Schiff bases were reacted with iodoacetic acid to create a new class of thiazolidinones. The synthesis of 4-Chloro coumarin 2 and 4-Chloro-3, 4', 3', 4"-tercoumarin 2a was carried out by combining 4-hydroxy coumarin and phosphorus pentachloride (PCl5). Moreover, the combination of toluenediamine sulfate and compound 2 led to the formation of 4-[(4-aminophenyl) amino]-2H-chromen-2-one. To create various Schiff bases of coumarin, 4-[(4-aminophenyl) amino]-2H-chromen-2-one was condensed with different aldehydes. The synthesized compounds' structures were verified using techniques such as IR, 1H NMR, 13C NMR, and elemental analyses (C, H, N).
Results: This investigation suggests the potential of the compound like synthesis of 4-Chloro coumarin 2, 4-Chloro-3, 4', 3', 4"-tercoumarin 2a, and 4-[(4-aminophenyl) amino]-2H-chromen-2-one demonstrated the successful creation of novel compounds through specific reactions. The condensation of 4-[(4-aminophenyl) amino]-2H-chromen-2-one with various aldehydes led to the generation of distinct Schiff bases of coumarin.
Conclusion: In conclusion, the study highlighted the significance of small ring heterocycles containing nitrogen and sulfur, particularly thiazoles, in the development of important pharmaceutical compounds. The successful synthesis of novel compounds through specific reactions underscores the versatility of the methods employed. The structural verification of the newly synthesized compounds further supports the validity of the study's findings.
Effectiveness of personalized meal recommendation in improving dietary behaviors of Chinese community-dwelling elders: study protocol for a randomized controlled trial
Authors: Xu Zidu , Xu Xiaowei , Sun Lianglong , Guo Zhen , Lai Jianqiang , Kang Lin , Li Jiao 
Published: September 2023

Abstract
Background:
Inappropriate eating behaviors, particularly a lack of food diversity and poor diet quality, have a significant impact on the prognosis of certain chronic conditions and exacerbate these conditions in the community-dwelling elderly population. Current dietary interventions for the elderly have not adequately considered the nutritional needs associated with multiple chronic conditions and personal dietary preferences of elderly individuals. A personalized recommendation system has been recognized as a promising approach to address this gap. However, its effectiveness as a component of an elderly-targeted dietary intervention in real- world settings remains unknown. Additionally, it is unclear whether this intervention approach will be user-friendly for the elderly. Therefore, this
study aims to examine the effectiveness of a personalized meal recommendation system designed to improve dietary behavior in community-dwelling elders. The implementation process in terms of System usability and satisfaction will also be assessed.
Methods:
The trial has been designed as a 6-month, non-blinded, parallel two-arm trial. 150 community-dwelling elders who meet the eligibility criteria will be enrolled. Subjects will be allocated to either the intervention group, receiving personalized meal recommendations and access to corresponding food provided as one component of the intervention, as well as health education on elder nutrition topics, or the control group, which will receive nutritional health education lectures. Outcomes will be measured at three time points: baseline at 0 months, 3 months, and 6 months. The primary outcomes will include dietary diversity (DDS) and diet quality (CDGI-E) of enrolled community-dwelling elders, representing their dietary behavior improvement, along with dietary behavior adherence to recommended meals. Secondary outcomes will measure the perceived acceptability and usability of the personalized meal recommendation system for the intervention group. Exploratory outcomes will include changes in the nutritional status and anthropometric measurements of the community-dwelling elders.
Discussion:
This study aims to examine the effectiveness, acceptability, and usability of a personalized meal recommendation system as a data-driven dietary intervention to benefit community-dwelling elders. The successful implementation will inform the future development and integration of digital health strategies in daily nutrition support for the elderly.
Trial registration:
Chinese Clinical Trial Registry ChiCTR2300074912. Registered on August 20, 2023, https://www.chictr.org.cn/showproj.html?proj=127583
Mediated democracy in Action
Authors: Taglo Belew Anley , Moges Mulatu Alemayehu , Skjerdal Terje 
Published: September 2023

Abstract
Using media democracy as its case, this article reports results of a study on the state of Ethiopian newspapers with op-ed commentaries. There is good reason to focus on newspaper op-ed column, because it is considered as a forum for democratic practice in a mediated setting. Thus, the study takes the perspective of op-ed forum as a public sphere where citizens articulate issues of public matters. Guided by two major research questions, the study empirically examines the salient features of media democracy focusing on op-ed articles published during the 2018 Ethiopian political reform period, which range in date from April 2, 2018 to April 1, 2019.
The study employs a content analysis research method. Applying quantitative approach, the study employed a constructed week sampling method to select quantitative data from sample newspapers. The study focuses on op-ed content and source features, which have been taken as key characteristics of op-ed page to measure ‘what writers said’ and ‘whose voices were heard’ regarding the 2018 Ethiopian political reform. In terms of diversity, the study disclosed a limited diversity in terms of source and content.
The study reported in this article shows that media democracy in the op-ed pages of Ethiopian newspapers, particularly in state-owned newspapers, were constructed to advance the newspapers’ editorial stance on the issues. Looking at the features of op-ed columns vis-à-vis editorial lines, the study finds that most op-ed commentaries bear similarity to editorials in state-controlled newspapers (coincide), and in private newspapers op-eds neither coincide nor collide with editorial lines the papers pursue.
Increasing variance of rich-club nodes distribution in EOD according to dynamic network
Authors: Mai Naikeng , Wu Yujie , Zhong Xiaomei , Chen Ben , Zhang Min , Peng Qi , Ning Yuping 
Published: September 2023

Abstract
Early onset depression(EOD) and late onset depression(LOD) are thought to have different pathogeneses, but lack of pathological evidence. In the current study we describe the dynamic rich-club properties of patients with EOD and LOD to address this question indirectly.We recruited 82 patients with late life depression (EOD 40, LOD 42) and 90 healthy controls. Memory, executive function and processing speed were measured, and resting-stage functional MRI was performed with all participants. We constructed a dynamic functional connectivity network and carried out rich-club and modularity analyses. Normalized mutual information(NMI) was applied to describe the variance in rich-club nodes distribution and partitioning. The NMI coefficient of rich club nodes distribution among the three groups was the lowest in the EOD patients (F = 4.298; P=0.0151, FDR=0.0231), which was positively correlated with rich-club connectivity (R = 0.886,P<0.001) and negatively correlated with memory(R = -0.347,P = 0.038) in the EOD group. In the LOD patients, non-rich-club connectivity was positively correlated with memory(R = 0.353,P = 0.030 and R = 0.420,P = 0.009). Furthermore, local connectivity was positively correlated with processing speed in the LOD patients (R = 0.374,P = 0.021). The modular partition was different between the EOD patients and the HCs(P=0.0013 < 0.05/3). The temporal instability of rich-club nodes was found in the EOD patients, but not the LOD patients, supporting the hypothesis that EOD and LOD result from different pathogenesis, and showing that the instability of the rich-club nodes across time might disrupt rich-club connectivity.
Framing of Public Media Input in a Mediated Deliberation Context
Authors: Taglo Belew Anley , Moges Mulatu Alemayehu , Skjerdal Terje 
Published: September 2023

Abstract
The Ethiopia of 2018 was a country experiencing political reform from the three-years youth-led mass protest and violence. The reform became one of the top concerns in the Ethiopian media between 2018 and 2019. This study aims to determine media framing of the reform in major Ethiopian newspapers with public media input of op-ed commentaries.
The purpose of this study is to examine how the media constructed the Ethiopian political reform which happened in 2018. Thus, the study uses the paradigms of constructivism, framing analysis and construction of media reality. The mass media studied were four major national newspapers. Applying qualitative approaches, data were collected through a constructed week sampling method, which is important to approximate media content for larger population of textual data.
For the data analysis and findings of this research, an issue-based textual analysis was conducted to identify frames appearing in op-ed guest articles between 2018 and 2019 surrounding the political reform. The subject of the analysis is the framing of how the political reform case was played out in major national newspapers in the specified period.
In a mediated deliberation, focusing on op-ed guest commentary as a sources of framing, the study identified ‘attribution of responsibility frame’ and ‘binary frame’ as key frames in the studied material. Attribution of responsibility frame, in conjunction with prognostic framing function, employed both prime minister-level attribution of responsibility and societal-level attribution of responsibility, in the selected stories. Taking frame-building process, both internal factors (in private newspapers) and external factors (in state-run newspapers) have determined the identified frames in the study.