bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD38‐Specific CAR Integrated into CD38 Locus Driven by Different Promoters Causes Distinct Antitumor Activities of T and NK Cells
Authors: Liao Chan , Wang Yajie , Huang Yanjie , Duan Yanting , Liang Yan , Chen Jiangqing , Jiang Jie , Shang Kai , Zhou Chun , Gu Ying , Liu Nan , Zeng Xun , Gao Xiaofei , Tang Yongmin , Sun Jie 
Published: July 2023

AbstractThe robust and stable expression of CD38 in T‐cell acute lymphoblastic leukemia (T‐ALL) blasts makes CD38 chimeric antigen receptor (CAR)‐T/natural killer (NK) a potential therapy for T‐ALL. However, CD38 expression in normal T/NK cells causes fratricide of CD38 CAR‐T/NK cells. Here a “2‐in‐1” gene editing strategy is developed to generate fratricide‐resistant locus‐specific CAR‐T/NK cells. CD38‐specific CAR is integrated into the disrupted CD38 locus by CRISPR/Cas9, and CAR is placed under the control of either endogenous CD38 promoter (CD38KO/KI) or exogenous EF1α promoter (CD38KO/KIEF1α). CD38 knockout reduces fratricide and allows the expansion of CAR‐T cells. Meanwhile, CD38KO/KIEF1α results in higher CAR expression than CD38KO/KI in both CAR‐T and CAR‐NK cells. In a mouse T‐ALL model, CD38KO/KIEF1α CAR‐T cells eradicate tumors better than CD38KO/KI CAR‐T cells. Surprisingly, CD38KO/KI CAR‐NK cells show superior tumor control than CD38KO/KIEF1α CAR‐NK cells. Further investigation reveals that endogenous regulatory elements in NK cells lead to higher expression of CD38 CAR than in T cells, and the expression levels of CAR affect the therapeutic outcome of CAR‐T and CAR‐NK cells differently. Therefore, these results support the efficacy of CD38 CAR‐T/NK against T‐ALL and demonstrate that the “2‐in‐1” strategy can resolve fratricide and enhance tumor eradication, paving the way for clinical translation.
A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films
Authors: Yadid Moran , Hagel Mario , Labro Megan Beldjilali , Le Roi Baptiste , Flaxer Carina , Flaxer Eli , Barnea A. Ronny , Tejman‐Yarden Shai , Silberman Eric , Li Xin , Rauti Rossana , Leichtmann‐Bardoogo Yael , Yuan Hongyan , Maoz Ben M. 
Published: July 2023

AbstractDespite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length‐based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility.
Bioorthogonal CRISPR/Cas9‐Drug Conjugate: A Combinatorial Nanomedicine Platform
Authors: Beha Marcel Janis , Kim Joo‐Chan , Im San Hae , Kim Yunsu , Yang Seungju , Lee Juhee , Nam Yu Ri , Lee Haeshin , Park Hee‐Sung , Chung Hyun Jung 
Published: July 2023

AbstractBioconjugation of proteins can substantially expand the opportunities in biopharmaceutical development, however, applications are limited for the gene editing machinery despite its tremendous therapeutic potential. Here, a self‐delivered nanomedicine platform based on bioorthogonal CRISPR/Cas9 conjugates, which can be armed with a chemotherapeutic drug for combinatorial therapy is introduced. It is demonstrated that multi‐functionalized Cas9 with a drug and polymer can form self‐condensed nanocomplexes, and induce significant gene editing upon delivery while avoiding the use of a conventional carrier formulation. It is shown that the nanomedicine platform can be applied for combinatorial therapy by incorporating the anti‐cancer drug olaparib and targeting the RAD52 gene, leading to significant anti‐tumor effects in BRCA‐mutant cancer. The current development provides a versatile nanomedicine platform for combination treatment of human diseases such as cancer.
Novel Fabrication of Basalt Nanosheets with Ultrahigh Aspect Ratios Toward Enhanced Mechanical and Dielectric Properties of Aramid Nanofiber‐Based Composite Nanopapers
Authors: Ji Dexian , Song Shunxi , Lyu Yuming , Ren Wei , Li Linghao , Yang Bin , Zhang Meiyun 
Published: July 2023

AbstractThe rapid development of modern electrical equipment has led to urgent demands for electrical insulating materials with mechanical reliability and excellent dielectric properties. Herein, basalt nanosheets (BSNs) with high aspect ratios (≈780.1) are first exfoliated from basalt scales (BS) through a reliable chemical/mechanical approach. Meanwhile, inspired by the layered architecture of natural nacre, nacre‐mimetic composite nanopapers are reported containing a 3D aramid nanofibers (ANF) framework as a matrix and BSNs as ideal building blocks through vacuum‐assisted filtration. The as‐prepared ANF‐BSNs composite nanopapers exhibit considerably enhanced mechanical properties with ultralow BSNs content. These superiorities are wonderfully integrated with exceptional dielectric breakdown strength, prominent volume resistivity, and extremely low dielectric constant and loss, which are far superior to conventional nacre‐mimetic composite nanopapers. Notably, the tensile strength and breakdown strength of ANF‐BSNs composite nanopapers with a mere 1.0 wt% BSNs reach 269.40 MPa and 77.91 kV mm−1, respectively, representing an 87% and 133% increase compared to those of the control ANF nanopaper. Their properties are superior to those of previously reported nacre‐mimetic composite nanopapers and commercial insulating micropapers, indicating that ANF‐BSNs composite nanopapers are a highly promising electrical insulating material for miniaturized high‐power electrical equipment.
A new look at agricultural fires and health: A replication of Rangel and Vogl (2019)
Authors: Gammans Matthew , Ortiz‐Bobea Ariel 
Published: July 2023

AbstractGlobally, over 400 million tons of biomass are burned in agricultural fires for management purposes each year, substantially affecting air quality (Korontzi et al., Global Biogeochemical Cycles 2006; 20; Yevich & Logan, Global Biogeochemical Cycles 2003; 17). Rangel and Vogl (Review of Economics and Statistics 2019; 101:616–630) provide evidence that agricultural fires in Brazil cause large declines in newborn health in downwind communities. We replicate this analysis and evaluate the sensitivity of their results to changes in the dataset and alternative modeling choices. Although accounting for the potential of spatial correlation in errors reduces precision of estimated effects, we find that their primary conclusions are robust to alternative specifications and reasonable placebo tests. We discuss how our findings may guide future research on the relationship between agricultural fires and health.
Associations between milk cortisol and activity of the immune system of milk
Authors: Anyim Rachael , Li Shanita , Armstrong Daniel , Spathis Rita , Wander Katherine 
Published: July 2023

AbstractObjectivesBoth the immune system of human milk and milk cortisol have complex short‐ and long‐term effects on child health and development. As understanding continues to grow of the independent effects of each of these components of milk, it is also important to investigate their intersection, including how milk cortisol affects the immune system of milk. We began this important endeavor through secondary analyses of archived milk specimens.MethodsParticipants were 31 lactating mothers from upstate New York. We estimated milk cortisol concentrations via enzyme immunoassay. We assessed milk proinflammatory cytokine (interleukin‐6, IL‐6) responses to pathogenic (Salmonella) and commensal (Escherichia, Lactobacillus, Bifidobacterium) bacteria via in vitro stimulation. We estimated ordered logistic regression models to assess associations between milk cortisol and IL‐6 responses to bacteria.ResultsMilk cortisol ranged from 0.098 to 1.007 μg/dL. Milk cortisol was positively associated with IL‐6 responses to S. enterica (B: 4.035; 95% CI: 0.674, 7.395) and B. breve (B: 3.675; 95% CI: 0.426, 6.924); this association persisted after controlling for child age. Results were less clear for associations between milk cortisol and IL‐6 responses to L. acidophilus (B: 2.318; 95% CI: −1.224, 5.859) and E. coli (B: 2.366; 95% CI: −0.960, 5.692).ConclusionsComplex interactions between cortisol and the immune system extend to milk. Milk cortisol was positively associated with proinflammatory responses to some bacteria in vitro. This may suggest that milk cortisol is causally upstream of protective immune activity.
Steuerung der reversiblen Faltung von Einzelketten‐Nanopartikeln durch sichtbares Licht
Authors: Maag Patrick H. , Feist Florian , Truong Vinh X. , Frisch Hendrik , Roesky Peter W. , Barner‐Kowollik Christopher 
Published: July 2023

AbstractIn der vorliegenden Arbeit stellen wir Einzelketten‐Nanopartikel (ENPs) vor, die sich mit sichtbarem Licht (λmax=415 nm) aus ihrem gefalteten Zustand vollständig zu dem entsprechenden linearen Ausgangspolymer entfalten lassen. Die ursprüngliche Faltung wird durch eine einfache Veresterungsreaktion des Polymerrückrats, bestehend aus Acrylsäure und Polyethylenglykol‐dekorierten Acrylateinheiten, erreicht. Die so eingeführten Bimaneinheiten ermöglichen eine photochemische Entfaltungsreaktion durch Esterspaltung. Die Faltung und das lichtinduzierte Entfalten läuft weitgehend ohne Nebenreaktionen ab und kann direkt mittels Größenausschlusschromatographie (GPC) sowie diffusionsgeordneter NMR‐Spektroskopie (DOSY) durch die Veränderung des hydrodynamischen Radius (RH) nachverfolgt werden. Besonders hervorzuheben ist die Reversibilität sowohl der Faltung als auch der lichtinduzierten Entfaltung, die durch den hohen Umsatz bei der photochemischen Esterspaltung erreicht werden kann. Da die Photolyse in wässrigem Medium abläuft, könnte sich das System für die Untersuchung der Entfaltung von ENPs im biologischen Kontext eignen.
Nano Proteolysis Targeting Chimeras (PROTACs) with Anti‐Hook Effect for Tumor Therapy
Authors: Zhang Ni‐Yuan , Hou Da‐Yong , Hu Xing‐Jie , Liang Jian‐Xiao , Wang Man‐Di , Song Zhang‐Zhi , Yi Li , Wang Zhi‐Jia , An Hong‐Wei , Xu Wanhai , Wang Hao 
Published: July 2023

AbstractProteolysis targeting chimera (PROTAC) is an emerging pharmacological modality with innovated post‐translational protein degradation capabilities. However, off‐target induced unintended tissue effects and intrinsic “hook effect” hinder PROTAC biotechnology to be maturely developed. Herein, an intracellular fabricated nano proteolysis targeting chimeras (Nano‐PROTACs) modality with a center‐spoke degradation network for achieving efficient dose‐dependent protein degradation in tumor is reported. The PROTAC precursors are triggered by higher GSH concentrations inside tumor cells, which subsequently in situ self‐assemble into Nano‐PROTACs through intermolecular hydrogen bond interactions. The fibrous Nano‐PROTACs can form effective polynary complexes and E3 ligases degradation network with multi‐binding sites, achieving dose‐dependent protein degradation with “anti‐hook effect”. The generality and efficacy of Nano‐PROTACs are validated by degrading variable protein of interest (POI) such as epidermal growth factor receptor (EGFR) and androgen receptor (AR) in a wide‐range dose‐dependent manner with a 95 % degradation rate and long‐lasting potency up to 72 h in vitro. Significantly, Nano‐PROTACs achieve in vivo dose‐dependent protein degradation up to 79 % and tumor growth inhibition in A549 and LNCap xenograft mice models, respectively. Taking advantages of in situ self‐assembly strategy, the Nano‐PROTACs provide a generalizable platform to promote precise clinical translational application of PROTAC.
Evaluating convergent validity between RAISD and MSWO rankings across informants
Authors: Jeglum Sara R. , Melanson Isaac J. , O’Brien Matthew J. 
Published: July 2023

AbstractThe Reinforcer Assessment for Individuals with Severe Disabilities (RAISD) and multiple‐stimulus without replacement preference assessment (MSWO) are evidence‐based tools used to determine preferences indirectly through interview and observation of choice making, respectively. Often, the RAISD is used to inform the stimulus array of the MSWO. However, reports frequently vary between informants. This can lead to discrepant identification of putative reinforcers and therefore has the potential for weaker or ineffective reinforcement‐based treatments. The purpose of this study was to compare RAISD and MSWO outcomes across informants (adolescent, caregiver, and teacher) and evaluate the convergent validity between the two measures. The results indicated that across participants, RAISD and MSWO results rarely produced convergent validity. Implications for practitioners and researchers are discussed.
The effects of educational robotics in programming education on students' programming success, computational thinking, and transfer of learning
Authors: Yolcu Vehbi , Demirer Veysel 
Published: July 2023

AbstractThe study aims to investigate the effects of educational robotics (ER) in programming education on students' programming success, computational thinking (CT), and transfer of learning and to reveal the students' views about robotics programming. For this purpose, an explanatory sequential mixed‐method research design was used. In this research design, a qualitative data collection process follows a quantitative one to be able to further explain the novel results obtained through analysis of the quantitative data. In the quantitative part, a quasiexperimental study was designed, and one experimental group (EG) and one control group (CG) were formed randomly. The study was conducted with sixth‐grade middle school students for 14 weeks. In the process, students were given project tasks based on some real‐life problems, and they were asked to transfer their programming skills. It was preferred to use mBot as an ER. While the EG students performed programming activities with the mBot, the CG students performed only block‐based programming activities. Both groups were given pre‐ and posttests to collect data. Finally, semistructured interviews were conducted with the EG students to enrich quantitative findings. The results showed that robotics programming activities increased the students' programming success and transfer of learning, and scores of the EG were significantly higher than the CG. In addition, it was determined that both the EG and CG students' CT skills increased, but there was no significant difference between the groups. Finally, most of the students reported positive views on the use of ER in programming education.
The many “costs” of transportation: Examining what cancer caregivers experience as transportation obstacles
Authors: Thomson Maria D. , Van Houtven Courtney Harold , Xu Rebecca , Siminoff Laura A. 
Published: July 2023

AbstractBackgroundTransportation has been identified as a specific source of burden for cancer caregivers. This study examined cancer caregivers' subjective experiences and objectives costs associated with transportation over a 6‐month period of providing end‐of‐life care to a family member or friend.MethodsThis was a multi‐site longitudinal, prospective cohort study that followed 223 caregiver–patient dyads. Data were collected using biweekly, semi‐structured interviews for up to 6 months and collection of all caregiving related receipts. Interviews were coded and analyzed using a comparative, iterative analysis and actual out of pockets costs were described using descriptive statistics.ResultsOver the 6‐month study period most caregivers (n = 143; 74%) discussed transportation at one or more timepoints. Average biweekly transportations costs to caregivers were $43.6. Caregivers described (n = 56; 39%) multiple direct and indirect costs of transportation, and 58% (n = 84) discussed the need for transportations services or assistance at the institutional level.ConclusionsCaregivers described the multifaceted costs of transportation they experienced which are in line with previous work. Alongside descriptions of direct costs, caregivers described key opportunity costs, such as personal and work time forgone to transporting patients. Caregivers also made suggestions for institutional and/or civic based solutions to facilitate reliable modes of transportation, rather than individual‐level intervention.
An exploratory study of consumer switching behavior in platform businesses: A mixed methods approach
Authors: Ryu JungWon , Han Sang‐Lin , Park Taeyoung 
Published: July 2023

AbstractThis study investigates consumer switching behavior factors in platform businesses. This study reports a two‐phase study of switching behavior. The first phase, Latent Dirichlet Allocation topic analysis, was conducted by collecting reviews and comments to find out consumers' potential switching behavior factors. The second phase was the interview with the consumer who did the switching behavior as to why they made the switching behavior. Besides, among the potential switching behavior factors, actual switching behavior factors were compared. It links quantitative and qualitative phases in exploratory sequential mixed methods design. The switching behavior factors were identified by applying the push‐pull‐mooring framework. The result of the study on music platforms' push effects is price perception, pull effects are promotion and algorithm recommendation, and mooring effects are payment convenience, switching cost, and reward. In Over‐the‐top (OTT), platforms' push effect is a lack of content, pull effects are promotion, WOM, and diversity of content, and mooring effects are content expectations, social networks, payment convenience, and convenience of watching. In e‐commerce platforms' push effects are technical issues, delivery cost, and lack of trust, pull effects are service line extension, and convenience of use, mooring effects are reward, payment convenience, and convenience of access to my data. This work contributes to the growing body of studies investigating the switching behavior factor in each platform business. Also, the study makes recommendations to aid platform marketing managers in understanding the impact of push‐pull‐mooring variables.
A Tetradentate Ligand Enables Iron‐Catalyzed Asymmetric Hydrogenation of Ketones in a CO‐ or Isocyanide‐Free Fashion
Authors: Yang Lei , Tan Xuefeng , Zhao Menglong , Wen Jialin , Zhang Xumu 
Published: July 2023

AbstractWe herein reported the design and synthesis of a ferrocene‐based tetradentate ligand that is featured with modular synthesis and rigid skeleton. Its iron(II) complex facilitates asymmetric direct hydrogenation of ketones without the participation of extra strong‐field ligand such as CO and isocyanide. Hydride donor lithium aluminum hydride (LAH) converted non‐reactive Fe(II) species to reactive Fe(II) hydride species. With this catalyst, various chiral alcohols including the intermediate for montelukast could be prepared with satisfactory yields and enantioinduction.
Recycling von Silikonen ohne Qualitätsverlust
Authors: Sutthoff Rene 
Published: July 2023

AbstractMit viel Leidenschaft für das Thema Kreislaufwirtschaft und dem Antrieb, den CO2‐Fußabdruck von Silikon zu reduzieren und einem richtungweisenden “Magic Fit”, wie sie das Treffen mit Chemiker Stephan Enthaler nennen, haben die Gründerinnen von New Dawn Silicones, Tina Rose und Klara Yoon, eine ressourceneffiziente Recyclingtechnologie entwickelt, mit der Alt‐Silikone ohne Qualitätsverlust wiederverwendet werden können
Amorphous Ni−Mo−B−O Bifunctional Electrocatalyst for Simultaneous Production of Hydrogen and Value‐added Chemicals
Authors: Hao Xue , Cai Tong , Ma Jingxuan , She Guangwei , Zhang Haoyue , Wang Wei , Yu Jiacheng , Mu Lixuan , Shi Wensheng 
Published: July 2023

AbstractHydrogen evolution reaction (HER) coupled with biomass conversion is a sustainable route to produce clean energy H2 and value‐added chemicals simultaneously. Herein, an amorphous Ni−Mo−B−O bifunctional electrocatalyst was synthesized through a facile electrodeposition method and employed as a cathode for HER to produce H2 and as an anode for the conversion of hydroxymethylfurfural (HMF) to furandicarboxylic acid (FDCA). Besides leading to the formation of amorphous structures, the introduction of Mo and B can increase the electron density and optimize the electronic structure of the electrocatalyst, thus substantially increasing the catalytic activity of the catalyst. After continuous reaction at a constant potential of 0.58 V vs. Hg/HgO for 8 hours, the conversion of HMF reached 98.86 %, and the selectivity of the target product FDCA was as high as 92.97 %. Finally, a two‐electrolyzer system was constructed using the amorphous Ni−Mo−B−O as both cathode and anode to achieve simultaneous H2 production in the cathode chamber and FDCA production in the anode chamber at a low voltage. This work presents a promising strategy for the design and synthesis of high‐performance non‐noble metal electrocatalysts for efficient and cost‐effective H2 production.
DoE‐Assisted Development of a 2H−MoS2‐Catalyzed Approach for the Production of Indole Derivatives
Authors: Gentile Giuseppe , Morant‐Giner Marc , Cardo Lucia , Melchionna Michele , Fornasiero Paolo , Prato Maurizio , Filippini Giacomo 
Published: July 2023

Abstract2H−MoS2 is an appealing semiconductor because of its Earth‐abundant nature, cheapness, and low toxicity. This material has shown promising catalytic activity for various energy‐related processes, but its use in catalysis for C−C bond forming reactions towards useful organic compounds is still largely unexplored. The lack of examples in organic synthesis is mainly due to the intrinsic difficulties of using bulk 2H−MoS2 (e. g., low surface area), which implies the reliance on high catalytic loadings for obtaining acceptable yields. This makes the optimization process more expensive and tedious. Here, we report the development of a 2H−MoS2‐mediated synthesis of valuable bis(indolyl)methane derivatives, using indoles and benzaldehydes as starting materials. Exploiting the Design of Experiments (DoE) method, we identified the critical parameters affecting the catalytic performance of commercial 2H−MoS2 powder and optimized the reaction conditions. Lastly, we demonstrated that the catalytic system has versatility and good tolerance towards functional group variations of the reagents.
Know what it takes to advance to senior‐level positions
Authors: Calhoun Daniel W. , Tolman Steven 
Published: July 2023

As student affairs faculty, we equip our students with the knowledge and skills to be successful in their first job, but we also provide them with the tools to start thinking about their last job. For many, that last job is an executive position, such as director, dean, vice president, or president. One of the exercises we commonly conduct early in our programs and then revisit in the final semester is helping students create a professional development plan. As part of this exercise, students must assess their skillset and put it in the context of entry‐level and senior‐level jobs. This reflection includes reviewing employment postings in The Chronicle of Higher Education and HigherEdJobs.com to see the qualifications for their “dream job.”