bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic interventions on human xenografts promote systemic dissemination of oncogenes
Authors: Raghuram Gorantla V , Pal Kavita , Sriram Gaurav , Khan Afzal , Joshi Ruchi , Jadhav Vishalkumar , Shinde Sushma , Sheikh Alfina , Rane Bhagyeshri , Kangne Harshada , Mittra Indraneel 
Published: April 2023

AbstractWe generated xenografts of human cancer cells in mice, and using immuno-FISH analysis detected multiple co-localizing signals of human DNA and eight human oncoproteins in brain cells. Signals increased dramatically five days after treatment with chemotherapy, localized radiotherapy or surgery, which could be minimized by concurrent treatment with cell-free chromatin deactivating agents. These results suggest that therapeutic interventions may potentially encourage metastatic spread which is preventable by deactivating cell-free chromatin.
Early-life environmental effects on mitochondrial aerobic metabolism: an experimental brood size manipulation in wild great tits
Authors: Cossin-Sevrin Nina , Stier Antoine , Hukkanen Mikaela , Zahn Sandrine , Viblanc Vincent A. , Anttila Katja , Ruuskanen Suvi 
Published: April 2023

AbstractParental care (including postnatal provisioning) is a major component of the offspring’s early-life environment. In avian species, the number of chicks in the nest and subsequent sibling competition for food are known to affect chick’s growth, leading in some cases to long-lasting effects for the offspring. Because of its central role in converting energy, variation in the offspring’s mitochondrial metabolism could be an important pathway underlying variation in growth patterns. Here, we performed a brood size manipulation in great tits (Parus major) to unravel its impact on offspring’s mitochondrial metabolism and reactive oxygen species (ROS) production in red blood cells. We investigated the effects of brood size on chicks’ growth and survival, and tested for long-lasting effects on juvenile mitochondrial metabolism and phenotype. As expected, chicks raised in reduced broods had a higher body mass compared to enlarged and control groups. However, mitochondrial metabolism and ROS production were not significantly affected by the treatment either at chick or juvenile stages. Chicks in very small broods were smaller in size and had higher mitochondrial metabolic rates. The nest of rearing has a significant effect on nestling mitochondrial metabolism, yet variation in mitochondrial metabolism at the early-life stages are not associated with survival chances. The contribution of the rearing environment in determining offspring mitochondrial metabolism emphasizes the plasticity of mitochondrial metabolism in changing environments. Further studies would be needed to closely investigate what are the major environmental cues affecting the offspring mitochondrial metabolism during the growth period.
When is a slender not a slender? The cell-cycle arrest and scarcity of slender parasites challenges the role of bloodstream trypanosomes in infection maintenance
Authors: Larcombe Stephen D. , Briggs Emma M. , Savill Nick , Szoor Balazs , Matthews Keith R. 
Published: April 2023

SummaryThe development ofTrypanosoma bruceiin its mammalian host is marked by a distinct morphological change as replicative “slender” forms differentiate into cell-cycle arrested “stumpy” forms in a quorum-sensing dependent manner. Although stumpy forms dominate chronic infections at the population level, the proportion of replicative parasites at the individual cell level and the irreversibility of arrest in the bloodstream is unclear. Here, we experimentally demonstrate that developmental cell cycle arrest is definitively irreversible in acute and chronic infections in mice. Furthermore, analysis of replicative capacity and single-cell transcriptome profiling reveals a temporal hierarchy, whereby cell-cycle arrest and appearance of a stumpy-like transcriptome precedes irreversible commitment and morphological change. Unexpectedly, we show that proliferating parasites are exceptionally scarce in the blood after infections are established. This challenges the ability of bloodstream trypanosomes to sustain infection by proliferation or antigenic variation, these parasites instead being overwhelmingly adapted for transmission.
Foxp- and Skor-family proteins control differentiation of Purkinje cells from Ptf1a and Neurogenin1-expressing progenitors in zebrafish
Authors: Itoh Tsubasa , Uehara Mari , Yura Shinnosuke , Wang Jui Chun , Nakanishi Akiko , Shimizu Takashi , Hibi Masahiko 
Published: April 2023

ABSTRACTCerebellar neurons such as Purkinje cells (PCs) and granule cells (GCs) are differentiated from neural progenitors expressing proneural genes. Zebrafish mutants of proneural genesptf1aandneurogenin1showed a reduction or loss of PCs, GABAergic interneurons (INs), and reduced expression of GC progenitor genesatoh1a/b/c. Lineage tracing revealed that theptf1a-expressing progenitors gave rise to PCs, INs, and a part of GCs in zebrafish. These data indicate that theptf1a/neurognin1-expressing neural progenitors can generate a variety of cerebellar neurons. In this study, we found that genes encoding transcriptional regulators Foxp1b and Foxp4, as well as Skor1b and Skor2, which are reportedly expressed in PCs, were not expressed inptf1a;neurogenin1mutants.foxp1b;foxp4mutants showed a strong reduction in PCs, whileskor1b;skor2mutants completely lacked PCs but instead displayed an increase in immature GCs. Misexpression ofskor2in GC progenitors expressingatoh1csuppressed GC fate. These data indicate that Foxp1b/4 and Skor1b/2 function as key transcriptional regulators in the initial step of PC differentiation fromptf1a/neurogenin1-expressing neural progenitors, while Skor1b and Skor2 control PC differentiation by suppressing their differentiation into GCs.
Enhanced performance of gene expression predictive models with protein-mediated spatial chromatin interactions
Authors: Chiliński Mateusz , Lipiński Jakub , Agarwal Abhishek , Ruan Yijun , Plewczynski Dariusz 
Published: April 2023

AbstractThere have been multiple attempts to predict the expression of the genes based on the sequence, epigenetics, and various other factors. To improve those predictions, we have decided to investigate adding protein-specific 3D interactions that play a major role in the compensation of the chromatin structure in the cell nucleus. To achieve this, we have used the architecture of one of the state-of-the-art algorithms, ExPecto (J. Zhou et al., 2018), and investigated the changes in the model metrics upon adding the spatially relevant data. We have used ChIA-PET interactions that are mediated by cohesin (24 cell lines), CTCF (4 cell lines), and RNAPOL2 (4 cell lines). As the output of the study, we have developed the Spatial Gene Expression (SpEx) algorithm that shows statistically significant improvements in most cell lines.
Xrp1 governs the stress response program to spliceosome dysfunction
Authors: Stanković Dimitrije , Tain Luke , Uhlirova Mirka 
Published: April 2023

ABSTRACTCo-transcriptional processing of nascent pre-mRNAs by the spliceosome is vital to regulating gene expression and maintaining genome integrity. Here, we show that the deficiency of functional U5 snRNPs inDrosophilaimaginal cells causes extensive transcriptome remodeling and accumulation of highly mutagenic R-loops, triggering a robust stress response and cell cycle arrest. Despite compromised proliferative capacity, the U5 snRNP deficient cells increased protein translation and cell size, causing intra-organ growth disbalance before being gradually eliminated via apoptosis. We identify the Xrp1-Irbp18 heterodimer as the primary driver of transcriptional and cellular stress program downstream of U5 snRNP malfunction. Knockdown ofXrp1orIrbp18in U5 snRNP deficient cells attenuated JNK and p53 activity, restored normal cell cycle progression and growth, and inhibited cell death. Reducing Xrp1-Irbp18, however, did not rescue the splicing defects and the organismal lethality, highlighting the requirement of accurate splicing for cellular and tissue homeostasis. Our work provides novel insights into the crosstalk between splicing and the DNA damage response and defines the Xrp1-Irbp18 heterodimer as a critical sensor of spliceosome malfunction.
The pitfalls of negative data bias for the T-cell epitope specificity challenge
Authors: Dens Ceder , Laukens Kris , Bittremieux Wout , Meysman Pieter 
Published: April 2023

Summary / AbstractEven high-performing machine learning models can have problems when deployed in a real-world setting if the data used to train and test the model contains biases. TCR–epitope binding prediction for novel epitopes is a very important but yet unsolved problem in immunology. In this article, we describe how the technique used to create negative data for the TCR–epitope interaction prediction task can lead to a strong bias and makes that the performance drops to random when tested in a more realistic scenario.
The hepatokine FGL1 regulates hepcidin and iron metabolism during the recovery from hemorrhage-induced anemia in mice
Authors: Sardo Ugo , Perrier Prunelle , Cormier Kevin , Sotin Manon , Desquesnes Aurore , Cannizzo Lisa , Ruiz-Martinez Marc , Thevenin Julie , Billoré Benjamin , Jung Grace , Abboud Elise , Peyssonnaux Carole , Nemeth Elizabeta , Ginzburg Yelena Z. , Ganz Tomas , Kautz Léon 
Published: April 2023

ABSTRACTAs a functional component of erythrocyte hemoglobin, iron is essential for oxygen delivery to all tissues in the body. The liver-derived peptide hepcidin is the master regulator of iron homeostasis. During anemia, the erythroid hormone erythroferrone regulates hepcidin synthesis to ensure adequate supply of iron to the bone marrow for red blood cells production. However, mounting evidence suggested that another factor may exert a similar function. We identified the hepatokine FGL1 as a previously undescribed suppressor of hepcidin that is induced in the liver in response to hypoxia during the recovery from anemia and in thalassemic mice. We demonstrated that FGL1 is a potent suppressor of hepcidinin vitroandin vivo. Deletion ofFgl1in mice results in a blunted repression of hepcidin after bleeding. FGL1 exerts its activity by direct binding to BMP6, thereby inhibiting the canonical BMP-SMAD signaling cascade that controls hepcidin transcription.Key points1/ FGL1 regulates iron metabolism during the recovery from anemia.2/ FGL1 is an antagonist of the BMP/SMAD signaling pathway.
Process management: systematic review of determining factors for automation
Authors: Arantes Mayara Cassiano , Santos Silvio Francisco dos , Simão Victor Gomes 
Published: April 2023

PurposeThe objective of this article is to identify through a literature study which factors determine the success of process automation in management systems.Design/methodology/approachA systematic approach is applied to analyze the available literature on the topic. The first stage of the study consisted of analyzing publications aiming to identify aspects that may influence the implementation of automated process management tools in management systems. In the second stage, a synthesis of the information was performed, resulting in a set of representative factors of the aspects identified.FindingsAs a result of the literature review, determinant aspects were identified for the implementation of process automation in organizations in general. These elements are summarized in the following factors: Characterization and diversity of the context, Organizational Performance, People Engagement, Knowledge Management, Automated Process Management and Information Technology.Originality/valuePrior knowledge of the determinants and associated aspects has the potential to optimize process automation, as it eliminates unnecessary steps and allows better application of the available resources.
Ripple effect of disruptions on performance in supply chains: an empirical study
Authors: Duong An Thi Binh , Pham Tho , Truong Quang Huy , Hoang Thinh Gia , McDonald Scott , Hoang Thu-Hang , Pham Hai Thanh 
Published: April 2023

PurposeThe present study is performed to identify the propagation mechanism of the ripple effect as well as examine the simultaneous impact of risks on supply chain (SC) performance.Design/methodology/approachA theoretical framework with many hypotheses regarding the relationships between SC risk types and performance is established. The data are collected from a large-scale survey supported by a project of the Japanese government to promote sustainable socioeconomic development for the Association of Southeast Asian Nations (ASEAN) region, with the participation of 207 firms. Structural equation modeling (SEM) is used to test the hypotheses of the theoretical framework.FindingsIt is indicated that human-made risk causes operational risk, while natural risk causes both supply risk and operational risk. Furthermore, the impacts of human-made risk and natural risk on performance are amplified through operational risk.Research limitations/implicationsThis study is one of the first attempts that identifies the propagation mechanism of the ripple effect and examines the simultaneous impact of risks on performance in construction SCs.Originality/valueAlthough many studies on risk management in construction SCs have been carried out, they mainly focus on risk identification or quantification of risk impact. It is observed that research on the ripple effect of disruptions has been very scarce.
Digitalizing business models in hospitality ecosystems: toward data-driven innovation
Authors: Troisi Orlando , Visvizi Anna , Grimaldi Mara 
Published: April 2023

PurposeDigitalization accelerates the need of tourism and hospitality ecosystems to reframe business models in line with a data-driven orientation that can foster value creation and innovation. Since the question of data-driven business models (DDBMs) in hospitality remains underexplored, this paper aims at (1) revealing the key dimensions of the data-driven redefinition of business models in smart hospitality ecosystems and (2) conceptualizing the key drivers underlying the emergence of innovation in these ecosystems.Design/methodology/approachThe empirical research is based on semi-structured interviews collected from a sample of hospitality managers, employed in three different accommodation services, i.e. hotels, bed and breakfast (B&Bs) and guesthouses, to explore data-driven strategies and practices employed on site.FindingsThe findings allow to devise a conceptual framework that classifies the enabling dimensions of DDBMs in smart hospitality ecosystems. Here, the centrality of strategy conducive to the development of data-driven innovation is stressed.Research limitations/implicationsThe study thus developed a conceptual framework that will serve as a tool to examine the impact of digitalization in other service industries. This study will also be useful for small and medium-sized enterprises (SMEs) managers, who seek to understand the possibilities data-driven management strategies offer in view of stimulating innovation in the managers' companies.Originality/valueThe paper reinterprets value creation practices in business models through the lens of data-driven approaches. In this way, this paper offers a new (conceptual and empirical) perspective to investigate how the hospitality sector at large can use the massive amounts of data available to foster innovation in the sector.
The impact of personal reputation on leader emergence in autonomous work teams: theoretical considerations for future research
Authors: Horwitz Sujin K. 
Published: April 2023

PurposeResearch has demonstrated that varying degrees and combinations of individual traits, behaviors and characteristics influence the emergence of leaders in teams. While existing models have shown that such variables affect leader choice independently and interactively, the overall findings suggest that there is still a lack of research on a potentially significant factor, the impact of personal reputation on leader selection in autonomous work teams (AWTs), an increasingly ubiquitous team practice in organizations. This preliminary review thus aims to offer a general overview of personal reputation and its effects on leader emergence in the context of AWTs.Design/methodology/approachBy surveying extant literature on team leader emergence, this review has identified that the potential impact of personal reputation on leader selection in teams is significant yet largely ignored.FindingsModels of leader emergence in teams should incorporate the realistic assumption that teams formed in organizations are often comprised of individuals who have information on others either directly or indirectly. Personal reputation based on an individual’s history thus moderates how one’s behaviors or traits exhibited become contextualized in the overall assessment of leader desirability and selection in teams.Research limitations/implicationsBased on a review of existing research on leadership emergence and personal reputation, this work contends that the external validity and predictive value of leader emergence models would greatly benefit from the inclusion of employee reputations as a moderating variable in the future assessment of the leader emergence process in AWTs.Practical implicationsBy designing models that explain the potential effects of personal reputations on leader emergence in self-managed teams, team members and managers can be better elucidated and ultimately improve their understanding of the process of the evaluation and selection of team leaders.Originality/valueDespite the prolific research on leader emergence, there is still a paucity of studies examining personal reputational effects on leader selection, especially in the context of AWTs. This work is the first review piece calling for the inclusion of personal reputation, a substantive factor overlooked and excluded in previous models, to enhance the current understanding of leader emergence in AWTs.
Perceived employability in a situation of crisis: the influence of the external context and perceived financial threat
Authors: Silva Ana Maria , Gomes Jorge F.S. , Monteiro Sílvia 
Published: April 2023

PurposeThis study aims to analyse how people's perceived employability was affected during the coronavirus disease 2019 (COVID-19) pandemic. This study explores individuals' perceived financial threat, age and work situation as factors that shape perceived employability.Design/methodology/approachData were collected via a survey at three different times between October 2020 and May 2021, which were chosen to reflect the evolution of the pandemic. The participants (n = 124) reported participants' perceived employability and financial threat during the pandemic in Portugal. Perceived employability is a multidimensional concept, as this includes the following scales: employment protective behaviour, employment risk, job-seeking behaviour, self-control and self-learning.FindingsParticipants' overall perceived employability failed to record significant variance over the period under analysis. Nevertheless, perceived employment protective behaviour decreased the most, especially in the case of young adults (aged 18 to 24). Individuals' perceived financial threat varied according to the external context, being lower during the last moment of data collection, which corresponded to the less-socially and economically restrictive period. Employees with the most stable work condition, i.e. with a permanent employment contract, were those who felt less financially threatened when compared to other respondents. A negative relationship between perceived employability and perceived financial threat was identified during the third moment of data collection.Originality/valueThe research informs about how individuals perceive themselves in a highly unpredictable and unstable context. The longitudinal approach shows how the external context affected people's perceived employability and financial threat throughout the pandemic.
Re-examining the roles of experience quality at festivals: a comparative analysis using SEM and fsQCA
Authors: Saha Parmita , Nath Atanu , Sit Kokho (Jason) 
Published: April 2023

Purpose
The purpose of this study is to reexamine the dimensionality and role of experience quality (EQ) to explain other related factors, namely, perceived value, satisfaction and behavioral intention. Using dual methodological approaches of fuzzy set qualitative comparative analysis (fsQCA) and structural equations modeling (SEM), it seeks to establish the EQ’s construct and predictive validity.

Design/methodology/approach
Grounded in cross-sectional and online research design, the present study surveyed 881 attendees of an annual music festival (Malakoff) held in Norway and subjected the quantitative data to SEM and fsQCA.

Findings
Both SEM and fsQCA confirmed the socializing and enjoyment dimensions of EQ, with good validity and reliability, as well as the functional and emotional value dimensions explaining visitors’ perceived value of attending a festival. Both statistical analyses also showed that perceived value-derived satisfaction leads to behavioral intention regarding festival visits.

Practical implications
This study provides numerous valuable consequences for festival organizers, or marketers that can aid in developing effective strategies to measure visitors’ perceived quality of experience and numerous practical implications for festival organizers or marketers can help in developing effective strategies to measure visitors’ perceived quality of experience and then increase value perceptions, satisfaction and behavioral intentions toward attending festivals.

Originality/value
To the best of the authors’ knowledge, this study is among the first to adopt a dual-dimensional framework to measure EQ in festivals and establish its utility with two statistical approaches (SEM and fsQCA). It also shows the utility of perceived value, initially developed for adventure tourists, to study visitors’ experience with festivals.
Energy poverty and its impacts on health and education: a systematic review
Authors: Katoch Om Raj , Sharma Romesh , Parihar Sarita , Nawaz Ashraf 
Published: April 2023

Purpose
People with energy poverty are denied the modern energy services such as cooking, lighting, heating, cooling and communication. These needs are all crucial to maintaining an acceptable level of living standards. This paper aims to examine the effects of energy poverty on health and education.

Design/methodology/approach
This systematic review was conducted using the 2009 Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The ScienceDirect, Scopus and Google Scholar databases were used to search the studies conducted between 2012 and 2022. Studies included in this review were searched with some combinations of keywords and saved in Mendeley Desktop for review and referencing. Of 1,745 articles retrieved after removing the duplicates from the databases, 22 met the inclusion criteria.

Findings
Out of the total 22 studies reviewed, six were conducted in Asia, six in Europe, four in Africa, three in developing countries and one each in North America, Australia and at global level. Results indicated that impacts of energy poverty on health and education were negative. Efforts should be made to improve the economic conditions of the population in order to allow them access to energy services to achieve higher levels of living.

Practical implications
As this systematic review excludes non-peer-reviewed literature, case studies, reports and theses, and only includes studies published between 2012 and 2022 in English language only, consequently, it may not provide an exhaustive overview of the literature on topic.

Originality/value
To the best of the authors’ knowledge, this is the first systematic review to investigate the relationship between energy poverty, health and education conducted here. The search methodologies involve systematic searches of databases and other manual searches. Considering the wide inclusion criteria, this review is useful as a general overview of the issues and identifies particular gaps in the existing evidence.