bluuebunny/crossref_metadata_2025_split huggingface dataset:
High production of furfural by flash pyrolysis of C6 sugars and lignocellulose by Pd-PdO/ZnSO4 catalyst
Authors: Zhou Qiaoqiao , Gu Jinxing , Wang Jingwei , De Girolamo Anthony , Yang Sasha , Zhang Lian 
Published: March 2023

AbstractFurfural (C5H4O2) is an important platform chemical for the synthesis of next-generation bio-fuels. Herein, we report a novel and reusable heterogeneous catalyst, Pd-PdO/ZnSO4 with 1.1 mol% palladium (Pd), for the production of furfural by flash pyrolysis of lignocelluloses at 400 °C. For both dry and wet C6 cellulose and its monomers, the furfural yields reach 74–82 mol%, relative to 96 mol% from C5 xylan and 23–33 wt% from sugarcane bagasse and corncob. The catalyst has a well-defined structure and bifunctional property, comprising a ZnSO4 support for the dehydration and isomerization of glucose, and a local core-shell configuration for metallic Pd0 encapsulated by an oxide (PdO) layer. The PdO layer is active for the Grob fragmentation of formaldehyde (HCHO) from glucose, which is subsequently in-situ steam reformed into syn-gas (i.e. H2 and CO), whereas the Pd0 core is active in promoting the last dehydration step for the formation of furfural.
Heat transfer analysis of fractional model of couple stress Casson tri-hybrid nanofluid using dissimilar shape nanoparticles in blood with biomedical applications
Authors: Arif Muhammad , Di Persio Luca , Kumam Poom , Watthayu Wiboonsak , Akgül Ali 
Published: March 2023

AbstractDuring last decades the research of nanofluid is of great interest all over the World, particularly because of its thermal applications in engineering, and biological sciences. Although nanofluid performance is well appreciate and showed good results in the heat transport phenomena, to further improve conventional base fluids thermal performance an increasing number of researchers have started considering structured nanoparticles suspension in one base fluid. As to make an example, when considering the suspension of three different nanoparticles in a single base fluid we have the so called “ternary hybrid nanofluid”. In the present study three different shaped nanoparticles are uniformly dispersed in blood. In particular, the three different shaped nanoparticles are spherical shaped ferric oxide$$\text{Fe}_{3} \text{O}_{4}$$Fe3O4, platelet shaped zinc$$\left( \text{Zn} \right)$$Zn, and cylindrical shaped gold$$\left( \text{Au} \right)$$Au, which are considered in blood base fluid because of related advance pharmaceutical applications. Accordingly, we focused our attention on the sharp evaluation of heat transfer for the unsteady couple stress Casson tri-hybrid nanofluid flow in channel. In particular, we formulated the problem via momentum and energy equations in terms of partial differential equations equipped with realistic physical initial and boundary conditions. Moreover, we transformed classical model into their fractional counterparts by applying the Atangana–Baleanu time-fractional operator. Solutions to velocity and temperature equations have been obtained by using both the Laplace and the Fourier transforms, while the effect of physical parameters on velocity and temperature profiles, have been graphically analyzed exploiting MATHCAD. In particular, latter study clearly shows that for higher values of volume fraction$$\phi_{hnf}$$ϕhnfof the nanoparticles the fluid velocity declines, while the temperature rises for the higher values of volume fraction$$\phi_{hnf}$$ϕhnfof the nanoparticles. Using blood-based ternary hybrid nanofluid enhances the rate of heat transfer up-to 8.05%, spherical shaped$$\text{Fe}_{3} \text{O}_{4}$$Fe3O4enhances up-to 4.63%, platelet shaped$$\left( \text{Zn} \right)$$Znnanoparticles enhances up-to 8.984% and cylindrical shaped gold$$\left( \text{Au} \right)$$Aunanoparticles enhances up-to 10.407%.
Thermal tolerances of Popenaias popeii (Texas hornshell) and its host fish from the Rio Grande Basin, Texas
Authors: Rangaswami Xenia L. , Goldsmith Amanda M. , Khan Jennifer M. , Robertson Clinton R. , Lopez Roel R. , Randklev Charles R. 
Published: March 2023

AbstractFreshwater mussels are particularly sensitive to hydrologic changes, including streamflow and temperature, resulting in global decline. The Devils River in south-central Texas harbors the endangered freshwater mussel Popenaias popeii (Unionidae; Texas hornshell). There is concern that water withdrawals from the underlying aquifer may be negatively impacting this species. To assess this risk, we evaluated upper thermal tolerances (LT05 and LT50) of larvae (glochidia) and juveniles from two sites. After being acclimated to 27 °C, glochidia were subjected to five experimental temperatures (30, 32, 34, 36, and 38 °C) and non-acclimated control (20 °C) for 12-h and 24-h while juveniles were subjected to three experimental temperatures (30, 32, and 36 °C) and non-acclimated control (20 °C) for 96-h. We overlaid tolerance estimates against in situ water temperature and discharge data to evaluate thermal exceedances. Additionally, we reviewed upper thermal tolerances of P. popeii’s presumed host fish (Carpiodes carpio, Cyprinellas lutrensis, and Moxostoma congestum) and their congeners. Stream temperatures only occasionally exceeded mussel LT05/50 and fish CLMax/LTMax, likely due to the Devils River’s large spring input, highlighting the importance of protecting spring flows. We provide a practical framework for assessing hydrological needs of aquatic ectotherms, including the parasite-host relationship, which can be used to optimize environmental management.
Expression of olfactory receptor genes in non-olfactory tissues in the developing and adult zebrafish
Authors: Jundi Dania , Coutanceau Jean-Pierre , Bullier Erika , Imarraine Soumaiya , Fajloun Ziad , Hong Elim 
Published: March 2023

AbstractSince the discovery of olfactory receptor (OR) genes, their expression in non-olfactory tissues have been reported in rodents and humans. For example, mouse OR23 (mOR23) is expressed in sperm and muscle cells and has been proposed to play a role in chemotaxis and muscle migration, respectively. In addition, mouse mesencephalic dopaminergic neurons express various ORs, which respond to corresponding ligands. As the OR genes comprise the largest multigene family of G protein-coupled receptors in vertebrates (over 400 genes in human and 1000 in rodents), it has been difficult to categorize the extent of their diverse expression in non-olfactory tissues making it challenging to ascertain their function. The zebrafish genome contains significantly fewer OR genes at around 140 genes, and their expression pattern can be easily analyzed by carrying out whole mount in situ hybridization (ISH) assay in larvae. In this study, we found that 31 out of 36 OR genes, including or104-2, or108-1, or111-1, or125-4, or128-1, or128-5, 133-4, or133-7, or137-3 are expressed in various tissues, including the trunk, pharynx, pancreas and brain in the larvae. In addition, some OR genes are expressed in distinct brain regions such as the hypothalamus and the habenula in a dynamic temporal pattern between larvae, juvenile and adult zebrafish. We further confirmed that OR genes are expressed in non-olfactory tissues by RT-PCR in larvae and adults. These results indicate tight regulation of OR gene expression in the brain in a spatial and temporal manner and that the expression of OR genes in non-olfactory tissues are conserved in vertebrates. This study provides a framework to start investigating the function of ORs in the zebrafish brain.
The IgV domain of the poliovirus receptor alone is immunosuppressive and binds to its receptors with comparable affinity
Authors: Saha Shrayasee , Sparkes Amanda , Matus Esther I. , Lee Peter , Gariépy Jean 
Published: March 2023

AbstractPVR (poliovirus receptor) functions as a ligand that signals through TIGIT and CD96 to induce suppression of T-cell and NK-cell responses. Alternatively, PVR binds to CD226, resulting in a co-stimulatory signal. To date, TIGIT antibody antagonists have been developed to restore immune functions and allow PVR to signal though CD226 in the context of cancer immunotherapy. Due to PVR receptor heterogeneity, agonizing either of these pathways with a recombinant form of the PVR extracellular domain represents a therapeutic strategy for either immunosuppression or activation. Here, we developed a minimal murine PVR-Fc fusion construct, consisting of only the IgV domain of PVR (vdPVR-Fc), and assessed its ability to dampen inflammatory responses in a murine model of psoriasis. vdPVR-Fc and PVR-Fc containing the full-length extracellular domain bound to TIGIT, CD96 and CD226 with similar low nanomolar affinities as defined by surface plasmon resonance. vdPVR-Fc was also able to suppress the in-vitro proliferation of murine CD4+ and CD8+ T-cells in mixed splenocyte cultures. Importantly, vdPVR-Fc delayed the onset, and reduced inflammatory responses (scaling and thickness) in a murine model of psoriasis. Collectively, our results suggest that the minimal IgV domain of PVR is sufficient to dampen immune responses in-vitro and attenuate symptoms of psoriasis in-vivo.
Comprehensive characterization of maternal, fetal, and neonatal microbiomes supports prenatal colonization of the gastrointestinal tract
Authors: Park Jee Yoon , Yun Huiyoung , Lee Seung-been , Kim Hyeon Ji , Jung Young Hwa , Choi Chang Won , Shin Jong-Yeon , Park Joong Shin , Seo Jeong-Sun 
Published: March 2023

AbstractIn this study, we aimed to comprehensively characterize the microbiomes of various samples from pregnant women and their neonates, and to explore the similarities and associations between mother-neonate pairs, sample collection sites, and obstetrical factors. We collected samples from vaginal discharge and amniotic fluid in pregnant women and umbilical cord blood, gastric liquid, and meconium from neonates. We identified 19,597,239 bacterial sequences from 641 samples of 141 pregnant women and 178 neonates. By applying rigorous filtering criteria to remove contaminants, we found evidence of microbial colonization in traditionally considered sterile intrauterine environments and the fetal gastrointestinal track. The microbiome distribution was strongly grouped by sample collection site, rather than the mother-neonate pairs. The distinct bacterial composition in meconium, the first stool passed by newborns, supports that microbial colonization occurs during normal pregnancy. The microbiome in neonatal gastric liquid was similar, but not identical, to that in maternal amnionic fluid, as expected since fetuses swallow amnionic fluid in utero and their urine returns to the fluid under normal physiological conditions. Establishing a microbiome library from various samples formed only during pregnancy is crucial for understanding human development and identifying microbiome modifications in obstetrical complications.
Network-level analysis of ageing and its relationship with diseases and tissue regeneration in the mouse liver
Authors: Porukala Manisri , Vinod P. K. 
Published: March 2023

AbstractThe liver plays a vital role in maintaining whole-body metabolic homeostasis, compound detoxification and has the unique ability to regenerate itself post-injury. Ageing leads to functional impairment of the liver and predisposes the liver to non-alcoholic fatty liver disease (NAFLD) and hepatocellular carcinoma (HCC). Mapping the molecular changes of the liver with ageing may help to understand the crosstalk of ageing with different liver diseases. A systems-level analysis of the ageing-induced liver changes and its crosstalk with liver-associated conditions is lacking. In the present study, we performed network-level analyses of the ageing liver using mouse transcriptomic data and a protein–protein interaction (PPI) network. A sample-wise analysis using network entropy measure was performed, which showed an increasing trend with ageing and helped to identify ageing genes based on local entropy changes. To gain further insights, we also integrated the differentially expressed genes (DEGs) between young and different age groups with the PPI network and identified core modules and nodes associated with ageing. Finally, we computed the network proximity of the ageing network with different networks of liver diseases and regeneration to quantify the effect of ageing. Our analysis revealed the complex interplay of immune, cancer signalling, and metabolic genes in the ageing liver. We found significant network proximities between ageing and NAFLD, HCC, liver damage conditions, and the early phase of liver regeneration with common nodes including NLRP12, TRP53, GSK3B, CTNNB1, MAT1 and FASN. Overall, our study maps the network-level changes of ageing and their interconnections with the physiology and pathology of the liver.
A mixed model of heat exchange in stationary honeybee foragers
Authors: Stabentheiner Anton , Kovac Helmut 
Published: March 2023

AbstractDuring foraging honeybees are always endothermic to stay ready for immediate flight and to promote fast exploitation of resources. This means high energetic costs. Since energy turnover of foragers may vary in a broad range, energetic estimations under field conditions have remained uncertain. We developed an advanced model, combining the benefits of mechanistic and correlative models, which enables estimation of the energy turnover of stationary foragers from measurements of body surface temperature, ambient air temperature and global radiation. A comprehensive dataset of simultaneously measured energy turnover (ranging from 4 to 85 mW) and body surface temperature (thorax surface temperature ranging from 33.3 to 45 °C) allowed the direct verification of model accuracy. The model variants enable estimation of the energy turnover of stationary honeybee foragers with high accuracy both in shade and in sunshine, with SD of residuals = 5.7 mW and R2 = 0.89. Its prediction accuracy is similar throughout the main range of environmental conditions foragers usually experience, covering any combination of ambient air temperature of 14–38 °C and global radiation of 3–1000 W m−2.
Evaluation of the attractiveness of lips with different volumes after filling with hyaluronic acid
Authors: de Queiroz Hernandez Paula Martins , Cotrin Paula , Valarelli Fabricio Pinelli , de Oliveira Ricardo Cesar Gobbi , Bispo Carina Gisele Costa , Freitas Karina Maria Salvatore , Oliveira Renata Cristina , Cotrin Dra. Paula 
Published: March 2023

AbstractTo compare the attractiveness of lips with different volumes after filling in the view of laypersons, dentists, and specialists. The sample comprised close-up frontal and lateral photographs of the lips of 16 women who underwent lip filling procedure with hyaluronic acid and was divided into 2 groups. Group 1: eight women with thinner lips at pretreatment. Group 2: eight female patients with thicker lips at pretreatment. Photographs from before and 10 days after lip filling were used to assess attractiveness randomly displayed in a Google Forms questionnaire and then sent via a messaging app to the evaluators. The evaluators' groups were general dentists, dentists with degrees in facial aesthetics, and laypersons. The attractiveness was evaluated with scores from 0 to 10 (0: least attractive and 10 the greatest). T-tests were used for the statistical comparisons. The group with thinner lips showed significantly improved attractiveness after filling. The group with thicker lips showed a worsening attractiveness after filling. The group with thicker lips had significantly higher attractiveness scores than those with thinner lips. There was no significant difference in the preference between men and women. The group of laypersons was more rigorous, giving significantly lower lip attractiveness scores. Thinner lips showed a significant improvement in attractiveness after filling. Thicker lips showed a worsening of the attractiveness score after filling. Before and after filling, thicker lips had significantly higher attractiveness scores than thinner lips.Clinical relevance: The amount of fillers applied to each patient must be individually evaluated.
Human memory T cell dynamics after aluminum-adjuvanted inactivated whole-virion SARS-CoV-2 vaccination
Authors: Tavukcuoglu Ece , Yanik Hamdullah , Parveen Mubaida , Uluturk Sila , Durusu-Tanriover Mine , Inkaya Ahmet Cagkan , Akova Murat , Unal Serhat , Esendagli Gunes 
Published: March 2023

AbstractThis study evaluates the functional capacity of CD4+ and CD8+ terminally-differentiated effector (TEMRA), central memory (TCM), and effector memory (TEM) cells obtained from the volunteers vaccinated with an aluminum-adjuvanted inactivated whole-virion SARS-CoV-2 vaccine (CoronaVac). The volunteers were followed for T cell immune responses following the termination of a randomized phase III clinical trial. Seven days and four months after the second dose of the vaccine, the memory T cell subsets were collected and stimulated by autologous monocyte-derived dendritic cells (mDCs) loaded with SARS-CoV-2 spike glycoprotein S1. Compared to the placebo group, memory T cells from the vaccinated individuals significantly proliferated in response to S1-loaded mDCs. CD4+ and CD8+ memory T cell proliferation was detected in 86% and 78% of the vaccinated individuals, respectively. More than 73% (after a short-term) and 62% (after an intermediate-term) of the vaccinated individuals harbored TCM and/or TEM cells that responded to S1-loaded mDCs by secreting IFN-γ. The expression of CD25, CD38, 4-1BB, PD-1, and CD107a indicated a modulation in the memory T cell subsets. Especially on day 120, PD-1 was upregulated on CD4+ TEMRA and TCM, and on CD8+ TEM and TCM cells; accordingly, proliferation and IFN-γ secretion capacities tended to decline after 4 months. In conclusion, the combination of inactivated whole-virion particles with aluminum adjuvants possesses capacities to induce functional T cell responses.
Machine learning based combination of multi-omics data for subgroup identification in non-small cell lung cancer
Authors: Khadirnaikar Seema , Shukla Sudhanshu , Prasanna S. R. M. 
Published: March 2023

AbstractNon-small Cell Lung Cancer (NSCLC) is a heterogeneous disease with a poor prognosis. Identifying novel subtypes in cancer can help classify patients with similar molecular and clinical phenotypes. This work proposes an end-to-end pipeline for subgroup identification in NSCLC. Here, we used a machine learning (ML) based approach to compress the multi-omics NSCLC data to a lower dimensional space. This data is subjected to consensus K-means clustering to identify the five novel clusters (C1–C5). Survival analysis of the resulting clusters revealed a significant difference in the overall survival of clusters (p-value: 0.019). Each cluster was then molecularly characterized to identify specific molecular characteristics. We found that cluster C3 showed minimal genetic aberration with a high prognosis. Next, classification models were developed using data from each omic level to predict the subgroup of unseen patients. Decision‑level fused classification models were then built using these classifiers, which were used to classify unseen patients into five novel clusters. We also showed that the multi-omics-based classification model outperformed single-omic-based models, and the combination of classifiers proved to be a more accurate prediction model than the individual classifiers. In summary, we have used ML models to develop a classification method and identified five novel NSCLC clusters with different genetic and clinical characteristics.
Separation, reserve estimation and radioactivity responsibility of the economic heavy minerals of East El- Arish black sand, North Sinai, Egypt
Authors: Awad M. , El Mezayen A. M. , El Alfi S. M. , Ali H. H. , Sayyed M. I. , Hanfi M. Y. 
Published: March 2023

AbstractMulti-heavy mineral separation techniques like density, magnetic, and electromagnetic techniques are followed by picking, grain counting, and mineralogical examinations conducted with an environmental scanning electron microscope (ESEM). These techniques have been applied to several drill hole (well) composite samples representing beach sand and dunes of East El-Arish area, North Sinai, Egypt. The results explain the remarkable enrichment in total heavy minerals (THM) of beach sand (4.4%) compared to sand dunes (2.6%): this is due to the natural concentration of the currents of the Mediterranean Sea. After characterizing the heavy minerals in terms of the abundance of each mineral species, the mean values of content relative to total economic heavy minerals (TEHM) were determined as 70.03% ilmenite, 3.81% leucoxene, 3.03% magnetite, 8.7% garnet, 10.6% zircon, 3.13% rutile, 0.37% monazite, and 0.31% titanite in the sand dune samples. The total economic heavy minerals in the beach samples were 64.08% ilmenite, 1.6% leucoxene, 2.84% magnetite, 18.16% garnet, 10.4% zircon, 2.18% rutile, 0.61% monazite, and 0.13% titanite. Monazite, thorite, zircon, and apatite are the main radioactive minerals in the study area. The combination of two specific characteristics of the studied monazite (relatively high Th-U content and high frequency) makes monazite the main contributor to radioactivity of the study area.
Environmental transmission of Pseudogymnoascus destructans to hibernating little brown bats
Authors: Hicks Alan C. , Darling Scott R. , Flewelling Joel E. , von Linden Ryan , Meteyer Carol U. , Redell David N. , White J. Paul , Redell Jennifer , Smith Ryan , Blehert David S. , Rayman-Metcalf Noelle L. , Hoyt Joseph R. , Okoniewski Joseph C. , Langwig Kate E. 
Published: March 2023

AbstractPathogens with persistent environmental stages can have devastating effects on wildlife communities. White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans, has caused widespread declines in bat populations of North America. In 2009, during the early stages of the WNS investigation and before molecular techniques had been developed to readily detect P. destructans in environmental samples, we initiated this study to assess whether P. destructans can persist in the hibernaculum environment in the absence of its conclusive bat host and cause infections in naive bats. We transferred little brown bats (Myotis lucifugus) from an unaffected winter colony in northwest Wisconsin to two P. destructans contaminated hibernacula in Vermont where native bats had been excluded. Infection with P. destructans was apparent on some bats within 8 weeks following the introduction of unexposed bats to these environments, and mortality from WNS was confirmed by histopathology at both sites 14 weeks following introduction. These results indicate that environmental exposure to P. destructans is sufficient to cause the infection and mortality associated with WNS in naive bats, which increases the probability of winter colony extirpation and complicates conservation efforts.
Steady state oscillations of circular currents in concentric polariton condensates
Authors: Lukoshkin Vladimir , Sedov Evgeny , Kalevich Vladimir , Hatzopoulos Z. , Savvidis P. G. , Kavokin Alexey 
Published: March 2023

AbstractConcentric ring exciton polariton condensates emerging under non-resonant laser pump in an annular trapping potential support persistent circular currents of polaritons. The trapping potential is formed by a cylindrical micropillar etched in a semiconductor microcavity with embedded quantum wells and a repulsive cloud of optically excited excitons under the pump spot. The symmetry of the potential is subject to external control via manipulation by its pump-induced component. In the manuscript, we demonstrate excitation of concentric ring polariton current states with predetermined vorticity which we trace using interferometry measurements with a spherical reference wave. We also observe the polariton condensate dynamically changing its vorticity during observation, which results in pairs of fork-like dislocations on the time-averaged interferogram coexisting with azimuthally homogeneous photoluminescence distribution in the micropillar.