bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimicrobial resistance of Vibrio cholerae O1 strains isolated during cholera epidemics in eastern Democratic Republic of Congo between January 2011 and June 2022
Authors: NDEBA Patrick AYONGA , SHINGA Bruce WEMBULUA , WONE Fatimata , BARDIGUYO Alain-Bruno , DIALLO Lassina , MPINGA Jackson BYAMUNGU , KITUTU Henriette MULASI , SENGA Raphael KABANGWA KAKONGO , CISSE Viviane Marie-Pierre , TINE Roger Clément Kouly 
Published: January 2023

Abstract

Background
Although the mainstay of cholera treatment remains rehydration, treatment with antibiotics is recommended in serious forms and on specific sites. However, since the late 1970s, resistance to antibiotics in strains of Vibrio cholerae (Vc) has been observed and now threatens the effective treatment and control of cholera.
Objective and Methods
This cross-sectional, retro-prospective, descriptive and analytical study aims to describe the long-term evolution of the antibiotic resistance profile of Vibrio choleraeO1 strains isolated at the provincial public health laboratory of North Kivu during cholera epidemics in Eastern Democratic Republic of Congo (DRC) from January 1, 2011 to June 30, 2022.
Results
A total of 4832 isolates of Vibrio cholerae O1 were collected, more than half of them (72.46%, n=3175) in the province of North Kivu. Of the 3 serotypes, Vc O1 Inaba was the majority (57.6%, n=2522). Among the 4382 strains of Vc O1, very high levels of resistance were found to ampicillin (74.7%), nalidixic acid (83.8%), erythromycin (73.6%), chloramphenicol (68.8%) and Sulfamethoxazole/Trimethoprim (82%). A resistance of 30% (1316/4382) was found to doxycycline, a molecule recommended by the WHO as first-line in the treatment of cholera. We found 27 different resistance profiles (MDR) with respect to the 5 main molecules recommended in anticholera therapy. In the analytical study, we observed a statistically significant evolution over time (p=0.000) of these MDR profiles, including 5% in 2011 against nearly 40% between 2021 and 2022; with a distribution statistically dependent on age (p=0.0003) including 57.4% of cases in children under 15 (27% in those under 5); with a predominance (69%) in the province of North Kivu.
Conclusion
The resistance of Vibrio cholerae 01 to common antibiotics is high in eastern DRC and tends to increase over time. The abusive and inappropriate use of antibiotics is one of the major causes of this emergence of antimicrobial resistance. Close monitoring and measures for the proper use of antibiotics will be necessary to stem this scourge.
High regeneration may not contribute to the forest’s carbon storage: A case study in the mangrove forest of Rajang-Belawai-Paloh delta, Sarawak
Authors: Noweg Tonga , Nelson Julia , Lip H. M. , Yeo Shu Jun , Keleman Alfred , Philip Bridgette 
Published: January 2023

Abstract
The alarming rate of the mangrove ecosystem loss poses a threat of losing valuable carbon sinks. This study was conducted to (i) determine the trend of growth in different vegetation types and (ii) compare the aboveground biomass (AGB) and carbon storage in different vegetation types. The study was conducted at four vegetation types within the Rajang-Belawai-Paloh delta i.e., Matured Bakau-Berus Forest (MBBF), Bakau-Nipah Forest (BNF), Regenerating Forests (Debris pile) [RF-D], and Regenerating Forests (Machinery track) [RF-M]. Inventory plots (20 m X 20 m) are systematically located along the main waterways and smaller rivers/streams. Trees (≥5 cm diameter-at-breast height [DBH]), seedlings (<2 cm stem diameter), and saplings (2-4.9 cm stem diameter) were measured. The trend of total trees per hectare is found to be decreasing across the least disturbed vegetation (MBBF) to the most disturbed vegetation (RF-M). The trends of total seedlings and saplings per hectare are found to be going upwards from the least disturbed vegetation to the most disturbed vegetation. Kruskal-Wallis H-test showed that there is a significant difference in the AGB and carbon storage between different vegetation types, χ2(2) = 43.98, p = 0.00 with the highest mean rank AGB and carbon storage in BNF (612.20 t/ha) and lowest in RF-M (287.85 t/ha). It can be concluded that although the most disturbed vegetations have higher regeneration, it may not contribute to the forest’s carbon storage The naturally regenerated seedlings may not grow beyond the sapling stage unless sustainable forest management is conducted to ensure survivability and growth.
Moore-Penrose geometric Quasi-Mean For Conditional Operators.
Authors: Sohrabi Morteza , Jabbarzadeh Mohammad Reza 
Published: January 2023

Abstract
In this paper, we establish several inequalities concerning to theMoore-Penrose geometric quasi-mean A℗ν B=||BA†|ν A|2 for boundedconditional operator A and B in L2 (Σ), where A hasclosed range and ν≥ 0. In addition, we discuss some measuretheoretic characterizations for conditional operators in someoperator classes.
2010 Mathematics Subject Classification. Primary 47A63, 47A30.
Mitral valve transcriptome analysis in thirty-four age-matched Cavalier King Charles Spaniels with or without myxomatous mitral valve disease
Authors: Reimann Maria J. , Cremer Signe , Christiansen Liselotte , Ibragimov Emil , Gao Fei , Cirera Susanna , Fredholm Merete , Olsen Lisbeth H. , Karlskov-Mortensen Peter 
Published: January 2023

Abstract

We here report the results of a mitral valve transcriptome study designed to identify genes and molecular pathways involved in development of myxomatous mitral valve disease (MMVD) in dogs. The study is focused on a cohort of elderly age-matched dogs (n=34, age ~10 years) from a single breed – Cavalier King Charles Spaniels – with a high incidence of MMVD. The cohort comprises 19 dogs (10♀, 9♂) without MMVD, or with early stages of MMVD, and 15 dogs (6♀, 9♂) with congestive heart failure caused by MMVD. I.e. we compare gene expression in breed and age matched groups of dogs, which only differ with respect to severity of disease. We identify 56 genes, which are differentially expressed between the two groups. In this list of genes, we confirm an enrichment of genes related to the TNFβ signaling pathway, extracellular matrix organization, vascular development, and endothelium damage, which also have been identified in previous studies. However, the genes with the greatest difference in expression between the two groups areCNTN3andMYH1. Both genes encode proteins, which are predicted to have an effect on the contractile activity of myocardial cells, which in turn may have an effect on valvular performance and hemodynamics across the mitral valve. This may result in shear forces with impact on MMVD progression.
Health workers’ perspectives on the occurrence and management of antimicrobial resistance at the human-animal-environment interface in Uganda
Authors: Muleme James , Kibira Simon P.S. , Ssempebwa John C. , Mugambe Richard K. , Kankya Clovice , Munyeme Musso , Kisaka Stevens , Isunju John Bosco , Ninsiima Lesley Rose , Musoke David 
Published: January 2023

Abstract

Background
The role of human, animal and environmental health workers is increasingly being recognized and linked to the emergence and transmission of antimicrobial resistance (AMR). Little has been done in low and middle-income countries despite the seriousness of this public health challenge. Our study explored the perspectives of health workers on the occurrence, transmission, and management of AMR at the human-animal-environment interface.
Methods
We conducted a descriptive qualitative study within Wakiso district, central Uganda between August and October 2022, using focus group discussions (4) with village health teams (VHTs) and key informant interviews (12) with other cadres of health workers. Data were managed in NVivo 12 and analyzed using thematic network analysis exploiting both deductive and inductive approaches.
Findings:
Animal health workers had better knowledge of AMR occurrence compared to their counterparts from the human and environmental health sectors. VHTs had a limited understanding of the AMR concept, even though they believed that drug-resistant infections were a growing concern in the community. Advancement in technology was viewed as being both a facilitator and a barrier to AMR occurrence and management. Cultural norms underlying animal production and close interactions between humans and animals were reported as key drivers for AMR spillover and amplification. Laxity in policy implementation, unskilled human resource, and weak surveillance systems for AMR were reported to be the underlying gaps in its management. Most participants advocated for a One Health strategy in combatting AMR at a community level.
Conclusion
Culture-driven solutions, diagnosis before treatment, more sensitization of community health workers, and improved regulation of drug use as well as multi-sectoral collaborations may create a suitable environment for the implementation of one-health approaches in the management of AMR.
Higher incidence of new atrial fibrillation in hospitalised COVID-19 patients compared to lower respiratory tract infection, however, less patients anticoagulated at discharge
Authors: Induruwa Isuru , Cattermole Elizabeth , Paisey Christopher , Ne Colver Ken Howe , Khadjooi Kayvan 
Published: January 2023

Abstract
Background
Infection is a well-known contributor to developing cardiac arrythmias such as atrial fibrillation (AF), which contributes to over 25% of all ischaemic stroke. We wanted to quantify the incidence of first-diagnosed (new) AF (nAF) during hospitalisation with COVID-19 compared to a lower respiratory tract infection (LRTI), as well as compare anticoagulation rates at discharge, reasons for non-prescription of anticoagulation and determine factors associated with developing nAF with COVID-19.
Methods
We analysed a comprehensive hospital coding database on patients hospitalised due to COVID-19+/-AF or LRTI +/-AF, between 1/3/2020 and 31/12/2020 at a large tertiary hospital in the UK. Incidence of nAF during COVID-19 or LRTI, and the proportions of nAF patients discharged on anticoagulation and reasons for non-prescription from both cohorts were quantified.
Results
2243 patients were hospitalised with LRTI and 488 with COVID-19. nAF was diagnosed in significantly more COVID-19 patients compared to LRTI (7.0% vs 3.6%, P=0.003). However, significantly less COVID-19 patients were discharged on anticoagulation compared to LRTI (19.2% vs 55.9%, P=0.003) despite similar CHA2DS2-VASc scores, and lower ORBIT scores. 14/26 LRTI +nAF patients had documented contraindication not to be anticoagulated, whereas only 1/12 patients with COVID-19 +nAF did. Patients who developed nAF during hospitalisation with COVID-19 were older (P<0.001), had pre-existing congestive cardiac failure (P=0.004), ischaemic heart disease (IHD) or peripheral vascular disease (PVD) (P<0.001), and a higher CHA2DS2-VASc score (P=0.02). Older age (Odds ratio (OR) 1.03, P=0.007) and IHD/PVD (OR 2.87, P=0.01) increased the odds of developing nAF with COVID-19.
Conclusion
Higher incidence of nAF and lower anticoagulation rates in COVID-19 patients were observed, compared to LRTI. A larger proportion of COVID-19 +nAF patients did not have a clear documented reason for non-prescription of anticoagulation in their notes. Whilst we await further research and clear guidelines, a pragmatic approach would be to holistically consider anticoagulation in all patients with COVID-19+nAF and a high ischaemic stroke risk.
Resveratrol Prevents Apoptosis, allowing Preosteoblastic MC3T3-E1 cells to Proliferate and Differentiate
Authors: Cai Weiye , Song Chao , Liu Fei , Chen Rui , Gao Silong , Guo Daru , Mou Ning , Xiong Zhongwei , Liu Zongchao 
Published: January 2023

Abstract
The purpose of this study was to investigate the mechanism by which resveratrol (Res) inhibits apoptosis and promotes proliferation and differentiation of preosteoblastic MC3T3-E1, laying the groundwork for the treatment of osteoporosis (OP). The TCMSP database was used to find the gene targets for Res. The GeneCards database yielded the gene targets for OP. Following the discovery of potential gene targets, GO, KEGG, and Reactome enrichment analysis were conducted. Verifying the major proteins involved in apoptosis can bind to Res using molecular docking. CCK8 measured the proliferative activity of mouse preosteoblasts in each group following Res intervention. Alkaline phosphatase staining (ALP) and alizarin red staining to measure the degree of osteogenic differentiation. RT-qPCR to determine the expression levels of Runx2 and OPG genes for osteogenic differentiation ability of cells. Western blot to measure the degree of apoptosis-related protein activity in each group following Res intervention. The biological processes investigated for GO of Res therapeutic OP involved in cytokine-mediated signaling pathway, negative regulation of apoptotic process, Aging, extrinsic apoptotic signaling pathway in absence of ligand, according to potential therapeutic target enrichment study. Apoptosis, FoxO signaling pathway, and TNF signaling pathway are the primary KEGG signaling pathways. Recactome pathways are primarily engaged in Programmed Cell Death, Apoptosis, Intrinsic Apoptotic Pathway, and Caspase activation via extrinsic apoptotic signaling pathways. This research established a new approach for Res treatment of OP by demonstrating how Res controls the apoptosis-related proteins TNF, IL6, and CASP3 to suppress osteoblast death and increase osteoclastogenesis.
Phase I clinical trial of CD19 CAR-T cells expressing CXCR5 protein for the treatment of relapsed or refractory B-cell lymphoma
Authors: Wang Jiaxi , Jiang Yirong , Luo Min , Lu Wenyi , He Jixiang , Zhang Meng , Yao Zhuoxin , Jin Xin , Xiao Xia , Chen Jianhang , Li Guangchao , Ding Wen , Zhang Zhiyin , Zhao Mingfeng 
Published: January 2023

Abstract
Background
The difficulty of CD19 CAR-T cells entering solid tumors is one of the reasons for its poor efficacy in the treatment of lymphoma.The chemokine CXCL13 secreted by stromal cells of the lymph nodes, induces the homing of B and T lymphocytes who express its receptor CXCR5. Pre-clinical trials have shown that CD19 CAR-T cells expressing CXCR5 could increase its migration to the tumor microenvironment and enhance the anti-tumor function of CD19 CAR-T cells.
Methods
We generated a novel anti-CD19 CAR-expressing CXCR5 protein. Then, we conducted a phase I clinical trial to evaluate safety and efficacy of CXCR5 CD19 CAR-T cells in the treatment of relapsed or refractory (R/R) B-cell lymphoma.
Results
We recruited 10 patients with R/R B-cell lymphoma undergoing CXCR5 CD19 CAR-T cells therapy. The objective response rate was 80%, complete response rate was 50%. The progression-free survival of these ten patients was 5 months (95% CI 1.02-8.98 months), while the median overall survival was 17.76 months (95% CI 13.54-21.98 months). The incidence of grade 1 and grade 2 cytokine release syndrome (CRS) was 70% and 20%, respectively. No patient experienced grade 3 or higher level of CRS, neurotoxicity and infusion-related dose toxicity.
Conclusions
In this study, we suggest that the results obtained here can pave the way for CXCR5 CD19 CAR-T cells to be investigated in a trial with broader patient populations.
Trial registration
The trials were registered at www.chictr.org.cn as ChiCTR2100052677 and ChiCTR1900028692.
Influence of molybdenum and organic sources of copper and sulfur on performance and blood mineral concentration in lambs
Authors: Conti Renata M.C. , Silva Thiago H. , Guimarães Iuli C.S.B. , Bezerra Helena V.A. , Netto Arlindo Saran , Rodrigues Paulo H.M. , Pacheco José C.G. , Zanetti Marcus A. 
Published: January 2023

Abstract
This study aimed to evaluate the effects of molybdenum (Mo) and organic and inorganic sources of copper (Cu) and sulfur (S) on performance and blood concentration of these minerals in lambs. Forty male non-castrated crossbred Dorper x Santa Inês lambs (20 ± 1.2 kg of body weight and 90 ± 2 d of age) were randomly allocated into one of the 10 following treatments: T0) control, basal diet; T1) Mo; T2) inorganic Cu and inorganic S; T3) inorganic Cu and organic S; T4) organic Cu and inorganic S; T5) organic Cu and organic S; T6) Mo plus inorganic Cu and inorganic S; T7) Mo plus inorganic Cu and organic S; T8) Mo plus organic Cu and inorganic S; T9) Mo plus organic Cu and organic S. The animals were kept in individual pens and received a total mixed ration during 84 days. Body weight and blood sampling was performed every 28 days. All animals were slaughtered after 84 days and carcass traits were evaluated. No effects of treatments were observed on body weight and carcass traits. Dietary molybdenum and sulfur-supplemented animals had reduced serum concentrations of copper and ceruloplasmin. Also, an interaction among Mo x Cu sources x S sources was detected for both copper and ceruloplasmin serum concentrations. Organic sources of Cu and S led to increased serum concentration of S. Further, Mo supplementation influenced positively its blood concentration.
Culturally sensitive translation of the Perceived Stress Scale and the Multi-dimensional Scale of Perceived Social Support into Marathi language
Authors: Shidhaye Rahul , Thanage Chitra , Bangal Vidyadhar , Behere Nilam Shivajirao , Pulate Vandana , Tambe Sonali , Tilekar Swanand 
Published: January 2023

Abstract
Purpose: Perceived Stress Scale (PSS) and the Multi-dimensional Scale of Perceived Social Support (MSPSS) are widely used globally (and in India) to assess the perceived stress and perceived social support respectively. Local language versions of these tools have been validated in different parts of the world and some Indian languages, but these studies have been carried out in populations outside India. Translation and validation of these tools in Marathi language is not yet undertaken.
Methods: We used the following steps to translate and adapt the PSS and the MSPSS into Marathi: forward translation, feedback from the bilingual committee, cognitive interviewing and pre-testing, backward translation, comparison of the original English versions with the back-translated English versions, and the assessment of conceptual and operational equivalence which was done using the Pearson’s correlation coefficients and the Bland-Altman approach.
Results: There was a very high correlation between the English and the Marathi version of the PSS (0.97) and the MSPSS (0.98). For the self and interviewer administered versions, Pearson’s Correlation Coefficient was slightly lower for the PSS (0.76, p=0.01), but for MSPSS it was 0.97 (p<0.001). The agreement between the two language versions of both the scales and the self and interviewer-administered versions was also established using the Bland-Altman plots.
Conclusion: The outputs of this study, i.e., the self and interviewer-administered Marathi language versions of the PSS and the MSPSS are based on a systematic approach of culturally sensitive translation, and these can now be further used for assessment of their psychometric validity.
1,400 Years of Climate Change in the Northern Hemisphere
Authors: Morando Marco 
Published: January 2023

Abstract
Anthropogenic Global Warming is the Climate Change main cause. Nevertheless, several authors indicated the solar activity and the Atlantic Multi-decadal Oscillation variation as additional forcing. This article considers the amplification of the solar radiation and of the Atlantic Multi-decadal Oscillation’s variation, via sea ice cover albedo feedbacks in the Arctic regions, as forcing Climate change in complement to Anthropogenic Global Warming. A physical model, using the sunspot number count and the Atlantic Multi-decadal Oscillation index as inputs, simulates the average global temperature anomaly and the Arctic Sea Ice Extension for the past twelve centuries. This model represents innovative progress in understanding how existing studies on Arctic Sea ice’s albedo feedbacks can help complement the Anthropogenic Global Warming models, thus helping to define more precise models for future climate change. In addition, the North Atlantic Oscillation index across 1,200 years is determined and, through its correlation with temperature and precipitation anomaly, the climate history of 15 different European and Northern Hemisphere regions is modelled. The results are then compared with quantitative and qualitative benchmarks from the literature, to evaluate their level of accuracy in simulating the historical data. For purpose of simplicity, the model does not consider anthropogenic global warming. Nevertheless, the natural forcing described in this article shall be considered complementary to anthropogenic global warming effects. Manmade forcing will be included in a future model to be developed by the author.
Development of identification criteria for fruit vodkas (Part 1. Sample preparation ways)
Authors: Krikunova L. N. , Ulyanova E. V. , Tomgorova S. M. , Andrievskaya D. V. , Trofimchenko V. A. 
Published: January 2023

The development of reliable identification criteria for various types of foods, including fruit vodkas, is one of the top-priority directions of scientific research in the field of quality control. The review examines different approaches to solution to a problem of searching identification criteria for fruit vodkas that will allow differentiating products by a type of fruit raw materials, their grade and region of origin. To this end, instrumental methods of analysis were used, including spectral, high performance liquid chromatography (HPLC) and gas chromatography coupled to mass spectrometry (GC–MS) as the main method. When detecting minor aroma-forming substances using the latter method, it is necessary, first of all, to carry out the special sample preparation that includes extraction and concentration of target substances. The present review examines three main types of sample preparation (liquid extraction, solid-phase extraction, supercritical fluid extraction) and modifications of these methods. Their comparative analysis was carried out with respect to labor intensity, extraction effectiveness, volatile aroma-forming compounds significantly different by polarity, reproducibility and sustainability. It has been shown that a type of sample preparation affects reproducibility and sensitivity of an instrumental analytic method, which is especially important for identification of some minor compounds, which concentration can be regarded as indicators for identification of fruit raw materials. It has been concluded that among the examined methods of sample preparation, the most promising for the development of identification criteria for fruit vodkas is headspace solid-phase microextraction (HS-SPME) as this method is highly effective in terms of extraction of target components including minor.