bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Distinct Immune Signatures and Chemokine Networks in Scalp Inflammatory Diseases
Authors: Liu Baoyi , Li Ang , Liu Yi , Ke Xu , Liu Qingwu , Zuo Xianbo , Xu Jingkai , Cui Yong 
Published: June 2023

Abstract
Background
Alopecia areata (AA), cutaneous lupus erythematosus (CLE), and psoriasis are diseases that often affect the scalp. AA and CLE often lead to hair loss, whereas psoriasis does not. The underlying mechanisms contributing to these differential prognoses remain unclear.
Methods
Microarray datasets of the three scalp diseases were collected from the GEO database and were integrated by sva R package. Differentially expressed genes (DEGs) were identified by the limma R package. Generally Applicable Gene-set Enrichment (GAGE), CIBERSORT algorithm, and Gene set variation analysis (GSVA) was utilized to access the functional, immune infiltration, and T helper 1/2/17 Chemokine signature changes in diseases with or without hair loss. qRT-PCR, immunofluorescence, and immunohistochemical staining were used to detect gene expression alteration among diseases from patients and mouse models.
Results
We identified shared gene expression changes associated with T cell chemotaxis and interferon-β response in scalp autoimmune diseases. In addition to the expected reduction in intermediate and keratin filaments, four functional changes associated with alopecia were found, including intestinal immune network for IgA, cell adhesion molecules, natural killer cell-mediated cytotoxicity, and complement and coagulation cascades. Immune infiltration analysis revealed increased infiltration of CD8 + T cells, NK cells, and mast cells in AA and CLE, while CD4 + cells were the predominant infiltrating immune cells in scalp psoriasis. Furthermore, scalp psoriasis exhibited a distinct Th17/Th1 profile, elevated CCL4 levels, and more CCR5 + Foxp3 + cells infiltration around the hair follicle.
Conclusion
Our study identified shared pathways and immune cells involved in hair loss and revealed a prominent perifollicular infiltration model of CD4 + T cells and an increased CCL4-CCR5 axis in scalp psoriasis, which may contribute to hair preservation in psoriasis patients. These findings provided valuable insights for developing therapeutic strategies for inflammatory alopecia.
Relationship of irisin with disease severity and dopamine uptake in Parkinson's disease patients
Authors: Shi Xiaoxue , Gu Qi , Fu Chang , Ma Jianjun , Li Dongsheng , Zheng Jinhua , Chen Siyuan , She Zonghan , Qi Xuelin , Li Xue , Wu Shaopu , Wang Li 
Published: June 2023

Abstract
Background: This study was designed to investigate the relationship of irisin with the severity of Parkinson’s disease (PD) and dopamine (DOPA) uptake in patients with PD and to understand the role of irisin in PD and its potential value as a drug therapy.
Methods: The plasma levels of irisin and α-syn were measured by ELISA. Motor and nonmotor symptoms were assessed with related scales. DOPA uptake was measured with DOPA PET/MRI.
Results: The plasma level of α-syn and irisin in patients with PD increased and decreased gradually with the progression of the disease, respectively. There was a negative correlation between plasma α-syn and irisin levels in patients with PD. The level of irisin in plasma was negatively correlated with UPDRS-III scores and positively correlated with MOCA scores. The SORs of the ipsilateral and contralateral caudate nucleus, anterior putamen and posterior putamen in the high-Irisin group was significantly higher than those in the low-Irisin group, and irisin levels in the caudate nucleus, anterior putamen, and posterior putamen contralateral to the affected limb were lower than those on the ipsilateral side. The level of irisin was positively correlated with the SORs of the ipsilateral and contralateral caudate nucleus and putamen in PD patients.
Conclusions: Irisin plays a neuroprotective role by decreasing the level of α-syn. Irisin is negatively correlated with the severity of motor symptoms and cognitive impairment. More importantly, irisin can improve DOPA uptake in the striatum of patients with PD, especially on the side contralateral to the affected limb.
The Mediating Effects of Individual Resilience on the Outcomes of Caregiver Burden among Adult Children Caring for Parents with Advanced Cancer in China: a cross-sectional study
Authors: Chen Xian , Qiao Chengping , Arber Anne , Shen Yan , Rui Yehao , Zhang Rui , Pei Zhongling , Tang Yuanyuan , Sha Ziyu , Bao Jianying , Zhang Jia , Li Jinhua , Wang Dan , Wang Xuemei 
Published: June 2023

Abstract
Purpose
This study investigated the mediating role of individual resilience in the relationship between caregiver burden and quality of life (QoL) among Chinese adult children providing care to their parents with advanced cancer, with the aim to inform effective coping strategies and positive caregiving outcomes.
Methods
In a cross-sectional design, 614 caregivers from multiple centers, whose parents were undergoing chemotherapy and/or radiotherapy, completed questionnaires encompassing demographics, caregiver burden, symptoms of anxiety and depression, resilience, and QoL.
Results
Findings revealed a moderate level of caregiver burden among participants, significantly influenced by factors including education level, family income, single-child status, and participation in social media patient support groups. Caregivers who were only children or involved in patient support groups reported higher burden. Importantly, path analysis showed a significant impact of caregiver burden, anxiety, and depression on QoL, with these relationships being mediated by individual resilience.
Conclusions
Chinese adult child caregivers face a considerable burden, negatively influencing their QoL. Individual resilience, a modifiable factor, was identified as a critical mediator in this relationship, mitigating the negative implications of caregiver burden, anxiety, and depression. These findings underscore the need for caregiver interventions that consider not only demographics but also the socio-psychological dynamics of caregiving to enhance caregiver QoL.
N-nitrosomorpholine-induced Oncocytic Transformation in Rat Endocrine Organs
Authors: GEZER Emre , ÖZER Cüneyt , ŞİMŞEK Turgay , BAYRAK Büşra YAPRAK , TURAN Gupse , ÇETİNARSLAN Berrin , SELEK Alev , CANTÜRK Zeynep , SÖZEN Mehmet , KÖKSALAN Damla 
Published: June 2023

Abstract
Background N-nitrosomorpholine (NMO) is one of the most common N-nitroso compounds. An oncocytic transformation has been demonstrated in renal tubules of NMO-treated rats. In our study, we aimed to investigate the potential transformation of oncocytic cells in 6 endocrine organs, i.e., thyroid, adrenal and pituitary glands, pancreas, testis, and bone, of NMO-exposed rats.Methods Thirty male rats were born and raised. Fifteen of them were given a single dose of 320 mg NMO per kg body weight, dissolved in drinking water, by a gavage tube. At the end of 52 weeks, the animals in both series were sacrificed. Right after the sacrifice, 6 different endocrine organs (hypophysis, thyroid, pancreas, adrenal gland, bone [femur], and testicles) of each animal were excised.Results There was no evidence of oncocytic cell development in the control group. In contrast, oncocytes were observed in 8 out of 13 NMO-treated rats: 2 in the adrenal sections, 1 in the thyroid sections, 3 in the pituitary sections, and 2 in the pancreas sections. The cells in the testicle and bone sections were completely normal.Conclusions We showed that NMO induced an oncocytic change in pancreas, thyroid, pituitary, and adrenal glands. To date, no identified specific environmental risk factors that lead to an oncocytic transformation in endocrine glands have been reported previously. Given the increasing prevalence of endocrine-disrupting chemicals in the environment, personal care products, manufactured goods and food sources, there is a need to advance our understanding of the pathological mechanisms underlying oncocytosis in endocrine organs.
Cuproptosis-related LINC02454 as a biomarker for laryngeal squamous cell carcinoma based on a novel risk model and in vitro and in vivo analyses
Authors: Zhu Qingwen , Zhang Ruyue , Lu Fei , Zhang Xinyu , Zhang Daidi , Zhang Yaodong , Chen Erfang , Han Fugen , Zha DingJun 
Published: June 2023

Abstract
Purpose
Laryngeal squamous cell carcinomas (LSCCs) are aggressive tumors with the second-highest morbidity rate in patients with head and neck squamous cell carcinoma. Cuproptosis is a type of programmed cell death that impacts tumor malignancy and progression. The purpose of this study was to investigate the relationship between cuproptosis-related long non-coding RNAs (crlncRNAs) and the tumor immune microenvironment and chemotherapeutic drug sensitivity in LSCC, and crlncRNA impact on LSCC malignancy.
Materials and Methods
Clinical and RNA-sequencing data from patients with LSCC were retrieved from The Cancer Genome Atlas. Differentially expressed prognosis-related crlncRNAs were identified based on univariate Cox regression analysis, a crlncRNA signature for LSCC was developed and validated using LASSO Cox regression. Finally, the effect of LINC02454, the core signature crlncRNA, on LSCC malignancy progression was evaluated in vitroand in vivo.
Results
We identified a four-crlncRNA signature (LINC02454, AC026310.1, AC090517.2, and AC000123.1), according to which we divided the patients into high- and low-risk groups. The crlncRNA signature risk score was an independent prognostic indicator for overall and progression-free survival, and displayed high predictive accuracy. Patients with a higher abundance of infiltrating dendritic cells, M0 macrophages, and neutrophils had worse prognoses and those in the high-risk group were highly sensitive to multiple chemotherapeutic drugs. Knockdown of LINC02454 caused tumor suppression, via cuproptosis induction.
Conclusions
A novel signature of four crlncRNAs was found to be highly accurate as a risk prediction model for patients with LSCC andto have potential for improving the diagnosis, prognosis, and treatment of LSCC.
A randomized controlled trial to compare short-term outcomes following infragastric and infracolic omentectomy at the time of primary debulking surgery for epithelial ovarian cancer with normal-appearing omentum
Authors: Dong Xuhui , Yuan Lei , Zou Ruoyao , Yao Liangqing 
Published: June 2023

Abstract

Background
Omentectomy is an important procedure in surgery for epithelial ovarian cancer, but the scope of omentectomy is not recommended in the guidelines. This study was performed to evaluate the benefits and risks of infragastric omentectomy in patients with epithelial ovarian cancer.
Methods
This trial is a single center prospective study. Primary epithelial ovarian cancer patients with normal-appearing omentum were randomly assigned to either the control or experimental group and underwent infracolic or infragastric omentectomy, respectively. The primary endpoint was progression-free survival. This trial is registered on Chinese clinical trial registry site (ChiCTR1800018771).
Results
A total of 106 patients meeting the inclusion criteria for ovarian cancer were included during the study period. Of these, 53 patients underwent infracolic omentectomy, whereas 53 patients received infragastric omentectomy. Multivariate analysis revealed that infragastric omentectomy could improve the detection rate of omental metastases (OR: 6.519, P = 0.005). Infragastric omentectomy improved progression-free survival significantly for those cases with higher than stage IIB disease (HR: 0.456, P = 0.041). Based on the short-term results, infragastric omentectomy did not cause more perioperative complications.
Conclusions
Compared with infracolic omentectomy, infragrastric omentectomy may be a more appropriate surgical procedure for stage IIB-IIIC epithelial ovarian cancer patients with normal-appearing omentum.
Androgen deprivation therapy caused a drastic proliferation of B-cell lymphoma with IgG4-related disease in patients with prostate cancer: a case report
Authors: Sasagawa Hajime , Numakura Kazuyuki , Mori Mizuki , Kobayashi Mizuki , Kashima Soki , Yamamoto Ryohei , Nara Taketoshi , Saito Mitsuru , Narita Shintaro , Nanjo Hiroshi , Habuchi Tomonori 
Published: June 2023

Abstract
We report a case of diffuse large B-cell lymphoma that progressed rapidly after androgen deprivation therapy for prostate cancer in a patient with a history of IgG4-related disease. Estrogen has been reported to be a possible cause of acute exacerbations of malignant lymphoma. However, to date, this has only been reported in mouse models, with no reports of clinical cases, and its clinical significance has not been clarified. This case report describes a 75-year-old man with prostate cancer who had IgG4-related disease in the background. Hormone therapy was initiated to treat prostate cancer, but he developed dyspnea and back pain. Computed tomography showed enlargement of bilateral renal hilar masses, swelling of the lymph nodes in the mediastinum, and soft masses in the spinal canal. The specimens obtained by Computed tomography-guided biopsy of the mediastinal mass led to the diagnosis of diffuse large B-cell lymphoma. Immunohistochemistry was positive for estrogen receptor β, which led us to suspect rapid progression of diffuse large B-cell lymphoma due to estrogen suppression by Gonadotropin-releasing hormone antagonists. Hormone therapy was discontinued, and the patient received R-CHOP therapy. Subsequently, the lymphoma masses shrunk markedly, and the patient obtained remission. This case is the first report of clinical significance regarding the crucial role of estrogen and ERβ in malignant lymphoma in a patient with IgG4-related disease. This case report aims to raise awareness of the need to carefully select treatment options for prostate cancer patients with IgG4-related disease or lymphoma.
M gene targeted qRT-PCR approach for SARS-CoV-2 virus detection
Authors: Sarkar Md. Murshed Hasan , Naser Showti Raheel , Chowdhury Sanjana Fatema , Khan Md. Salim , Habib Md. Ahashan , Akter Shahina , Banu Tanjina Akhtar , Goswami Barna , Jahan Iffat , Nayem Maksudur Rahman , Hassan Md. Akibul , Rabbi Mohammad Fazle Alam , Ahsan Chowdhury Rafiqul , Miah Md. Ibrahim , Nessa Afzalun , Islam S M Rashed Ul , Rahman Mohammed Atiqur , Shaikh Md. Aftab Ali , Ahmed Md. Sharfuddin , Khan Md. Imran 
Published: June 2023

Abstract
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is the gold standard method for SARS-CoV-2 detection, and several qRT-PCR kits have been established targeting different genes of the virus. Due to the high mutation rate of these genes, false negative results arise thus complicating the interpretation of the diagnosis and increasing the need of alternative target. In this study, an alternative approach for the detection of SARS-CoV-2 viral RNA targeting the membrane (M) gene of the virus using qRT-PCR was described. Performance evaluation of this newly developed in-house assay against commercial qRT-PCR kits was done using clinical oropharyngeal specimens of COVID-19 positive patients. The limit of detection (LOD) was determined using successive dilutions of known copies of SARS-CoV-2 pseudovirus. The M gene based assay was able to detect a minimum of 100 copies of virus/mL indicating its capacity to detect low viral load. The assay showed comparable accuracy, sensitivity and specificity with commercially available kits while detecting all the variants efficiently. The study concluded that the in-house M gene based assay might be an effective alternative for the currently available commercial qRT-PCR kits.
Improving the image quality of short-time bone SPECT using CZT detectors with SwiftScan
Authors: Funakoshi Kazuto , Yamane Tomohiko , Kozawa Eito , Matsunari Ichiro 
Published: June 2023

Abstract
Objective This study aimed to evaluate the quality and associated quantitative values of bone single-photon emission computed tomography (SPECT) with and without SwiftScan using a semiconductor camera equipped with a cadmium-zinc-telluride (CZT) detector.Methods Ten patients with bone metastases from prostate cancer who underwent list-mode SPECT/computed tomography using a whole-body semiconductor camera were included in this study. A total dose of 740 MBq was administered. Standard SPECT images were taken approximately 3 h later, and images were constructed with and without SwiftScan.Results A total of 130 metastatic lesions in 10 patients were analyzed. The dose injected was 732.8 (662.3-878.1) MBq, and the time to start the test after injection was 200.5 (165།220) min. The intraclass correlation coefficient (ICC) was 0.952 at 5% for the standard time without SwiftScan and 0.990 with SwiftScan. The percentage of maximum standardized uptake value (SUVmax) changes of > 10% without SwiftScan was 0.7% at 100% of the images in the list mode data, 4.6% at 75%, 16.9% at 50%, 28.5% at 25%, 56.2% at 10%, and 68.4% at 5%. In contrast, with SwiftScan, SUVmax changes of > 10% were associated with 0.75% at 75%, 3.85% at 50%, 20.8% at 25%, 30.8% at 10%, and 40.8% at 5%. The contrast-to-noise ratios (CNR) were 95.3 at 75%, 88.3 at 50%, 69.2 at 25%, 45.7 at 10%, and 31.6 at 5% without SwiftScan, and were 96.9, 91.7, 78.0, 71.6, and 62.0, respectively, with SwiftScan.Conclusion SwiftScan can produce reliable images even with ultrashort acquisition times, and improves image quality and associated quantitative values. The method reported herein can be used to reduce SPECT inspection time.
Cross-species single-cell annotation with orthologous marker gene groups
Authors: Li Song , Chau Tran , Timilsena Prakash , Bathala Sai , Bargmann Bastiaan 
Published: June 2023

Abstract
The lack of known cell-type specific marker genes in most plants is a major hurdle for single-cell analysis. To address this challenge, we developed an approach to identify Orthologous Marker Gene groups (OMGs) across monocots and dicots and showed that they are capable of determining cell identities in tomato. This result indicates employing conserved OMGs from reference single-cell maps can annotate cell types in other plant species.
Early diastolic mitral annular velocity measured by tissue Doppler echocardiography renal and cardiovascular prognostic significance in IgA nephropathy
Authors: Sági Balázs , Késői István , Vas Tibor , Csiky Botond , Nagy Judit , Kovács Tibor József 
Published: June 2023

Abstract
Background.
In chronic kidney disease (CKD), as in IgA nephropathy (IgAN), cardiovascular (CV) mortality and morbidity are many times higher than in the general population, and diastolic dysfunction (LVDD) has prognostic significance as well. Tissue Doppler Echocardiography (TDI) is another method for measuring myocardial contractility and determining diastolic dysfunction.
Methods.
79 IgAN patients (age 46 ± 11 years) with CKD stages 1–3 were investigated and followed for 70 ± 28.7 months. Doppler echocardiography was used to measure the E (early) and A (late) waves, as well as the E wave deceleration time (EDT) during mitral inflow. TDI was used to measure early (Ea) and late (Aa) diastolic velocities (lateral and septal basal wall fragment average). From these, we calculated the E/Ea and Ea/Aa ratios. The primary combined endpoints were total mortality, major CV events, and end-stage renal disease, and the secondary endpoints were cardiovascular or renal.
Results.
Patients with decreased Ea (< 13 cm/s) had significantly more endpoints (20/42 vs. 3/37; p = 0.001) than patients with higher Ea (≥ 13 cm/s). The secondary renal endpoints were also significantly higher (p = 0.004). In a multivariate model, the eGFR showed independent correlation with the E/A ratio (r = 0.466; p < 0.01), EDT (r= -0.270; p < 0.01), Ea/Aa ratio (r = 0.455; p < 0.01), and decreased Ea (r = 0.544; p < 0.01). Independent factors influencing Ea were only EDT by uni- and multivariate regression but age and albuminuria by logistic regression.
Conclusion
Decreased Ea measured by TDI seems to be an eligible factor to predict the prognosis of IgA nephropathy. The decreased Ea may be a helpful parameter to identify high-risk CKD patients.
PET-CT in the staging of potentially resectable pancreatic cancer
Authors: saoudi Tareq Al , Chawla Kanika , Sarwar Dawud , Isherwood John , Bhardwaj Neil , Dennison Ashley , Garcea Giuseppe 
Published: June 2023

Abstract
Introduction:
Pancreatic adenocarcinoma (PDAC) presents a significant global health challenge particularly as it is generally diagnosed at an advanced stage. Although cross-sectional imaging techniques including multidetector CT (MDCT) are familiar and ubiquitously used the role and value of PET-CT scans in PDAC management remains unclear.
Methods:
This six-year study analysed patients who underwent PET-CT scans for suspected PDAC. Demographics, cross-sectional imaging and PET-CT results, management decisions, and histology were collected together with rates of operation with curative intent and the time interval between scans and surgical intervention.
Results:
Among 161 patients, 110 were operable with curative intent but in 51 no surgical intervention was appropriate or possible. Among patients considered operable, 20 had extra pancreatic PET avid lesions while 90 did not. Among those without extra-pancreatic lesions, 68 underwent surgical exploration, 12 were unfit for surgery, and 10 had inflammatory or benign conditions. In the group who underwent surgical exploration, 9 patients were found to have suspected metastasis prior to their PET scans which were not confirmed on subsequent PET-CT scans. Considering all patients who underwent an exploratory laparotomy 48 underwent successful resection and 20 were found to have disease which precluded potentially curative surgery. In the subset of patients with extra-pancreatic lesions, 4 were inoperable due to the demonstration of unequivocal metastasis on PET-CT but the remaining 16 patients underwent surgical exploration when the decision of the HPB MDT was that lesions were equivocal or may be unrelated to the primary pathology. Of these 16 patients, 9 were resectable and 7 were unresectable and PET-CT scans influenced the management of patients in 8% of cases. The median interval between MDCT and PET-CT scans was 27 days with an average of 36.7 days. The median duration between MDT and PET-CT scans was 12.5 days with an average of 20.2 days.
Conclusion:
PET-CT scans play a crucial role in the management of suspected PDAC, but routine use may not be justified. Further research is needed to identify the subset of patients who benefit most. Clear guidelines for PET scan utilization in clinical practice are required to optimize the role in PDAC management.