bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Spatiotemporal Characteristics of Atmospheric Water Cycle Processes over the Tibetan Plateau using the WRF Model and Finer Box Model
Authors: Pan Xiaoduo , Li Hu , Washakh Rana Muhammad Ali , Jin Jing 
Published: May 2023

Abstract
The Tibetan Plateau (TP) is the highest and largest plateau in the world and serves as a "vanguard" for global climate change. In the context of climate warming, changes in evapotranspiration (ET) and external water vapor transport cause more uncertainty in assessing the atmospheric water cycle processes over the TP. By using the Weather Research and Forecasting (WRF) model for long-term simulations and the finer box model for the calculation of water vapor along the boundary, the external atmospheric water vapor transport and its spatiotemporal characteristics over the TP are finely described. The model-simulated precipitation and evapotranspiration are well-simulated compared with observation. Research results show that: (1) The atmospheric water storage on the TP decreases from southeast to northwest. Water vapor is mainly transported into the TP from the western and southern boundaries. The net water vapor flux transported from the western boundary to the TP by westerly wind is negative, while the net water vapor flux transported from the southern boundary to the TP by southerly wind is positive. (2) In spring and winter, water vapor is mainly transported into the TP by mid-latitude westerlies from the western boundary. In summer, water vapor transport controlled by mid-latitude westerlies weakens, and water vapor is mainly transported into the TP from the southern boundary. In autumn, water vapor controlled by mid-latitude westerlies gradually strengthens, and water vapor is mainly transported into the TP from the western boundary. In addition, the ratio of ET to precipitation on the TP is about 0.48, and the moisture recycling is about 0.37. Water vapor mainly comes from external water vapor transport.
Temporal serum metabolomic and lipidomic analyses distinguish patients with access-related hand disability following arteriovenous fistula creation
Authors: Khattri Ram B. , Louis Lauryn Z. , Kim Kyoungrae , Anderson Erik M. , Fazzone Brian , Harland Kenneth C , Hu Qiongyao , O’Malley Kerri A. , Berceli Scott A. , Wymer James , Ryan Terence E. , Scali Salvatore T. 
Published: May 2023

Abstract
For end-stage kidney disease (ESKD) patients, hemodialysis requires durable vascular access which is often surgically created using an arteriovenous fistula (AVF). However, some ESKD patients that undergo AVF placement develop access-related hand dysfunction (ARHD) through unknown mechanisms. In this study, we sought to determine if changes in the serum metabolome could distinguish ESKD patients that develop ARHD from those that have normal hand function following AVF creation. Forty-five ESKD patients that underwent first-time AVF creation were included in this study. Blood samples were obtained pre-operatively and six-weeks post-operatively and metabolites were extracted and analyzed using nuclear magnetic resonance spectroscopy. Patients underwent thorough examination of hand function at both timepoints using the following assessments: grip strength manometry, dexterity, sensation, motor and sensory nerve conduction testing, hemodynamics, and the Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire. Nineteen of the forty-five patients displayed overt weakness using grip strength manometry (P < 0.0001). Unfortunately, the serum metabolome was indistinguishable between patients with and without weakness following AVF surgery. However, a significant correlation was found between the change in tryptophan levels and the change in grip strength suggesting a possible role of tryptophan-derived uremic metabolites in post-AVF hand-associated weakness. Compared to grip strength, changes in dexterity and sensation were smaller than those observed in grip strength, however, post-operative decreases in phenylalanine, glycine, and alanine were unique to patients that developed signs of motor or sensory disability following AVF creation.
SSR3 and SEC61G co-expression promotes proliferation in hepatocellular carcinoma cells
Authors: Liu Junjian , Zhang Ze , Mei Hanwei , Xu Yan , Zhang Jinghong , Bai Yali , Li Zhonglian , Yuan Hongxia 
Published: May 2023

Abstract
BACKGROUND Hepatocellular carcinoma (HCC) is a malignant tumor with a high incidence and poor prognosis. With the use of bioinformatics and next-generation sequencing technology, several molecular markers related to HCC diagnosis, treatment, and aetiology have been found. As an example, the upregulation of signal sequence receptor 3 (SSR3) has been linked to tumorigenesis. Studies also suggest that overexpression of SSR3 predicts poor survival in patients with HCC. However, research on the function and genes co-expressed with SSR3 is limited.METHODS The interaction between SSR3 and SEC61G proteins was analyzed using the STRING database. Correlation analysis of SSR3 and SEC61G mRNA levels was performed using the cBioPortal database. Expression levels of these two genes in HCC and normal tissues were evaluated, and the relationship with prognosis was analysed using the UALCAN database and tumor tissues obtained from surgical resection. Small interfering RNA targeting SSR3 or SEC61G, and overexpression vectors of SSR3 or SEC61G were transfected into HCC cells. SSR3 and SEC61G mRNA levels were detected using quantitative polymerase chain reaction, and a CCK-8 assay was performed to determine cell proliferation.RESULTS SSR3 and SEC61G mRNA levels were positively correlated (Spearman: 0.42, P ˂ 0.001), and the expression was increased in HCC tissues compared to that in normal tissues (P < 0.05). SSR3 knockdown decreased SEC61G mRNA levels. In contrast, SSR3 overexpression increased SEC61G mRNA levels. Higher SSR3 and SEC61G mRNA levels were associated with shorter overall survival (P < 0.01) and higher clinical stages (P < 0.05) in patients with HCC. Moreover, SSR3 and SEC61G co-expression promoted HCC cell proliferation (P < 0.01).CONCLUSION SSR3 co-expressed with SEC61G facilitated the proliferation of HCC cells and was associated with poor prognosis in patients with HCC.
Data Mining and Network Pharmacology-Based Exploration of the Mechanism of Action of Huangqin Qingre Chubi Capsule in Improving Immune Inflammation of Ankylosing Spondylitis
Authors: Zhang Fan , Liu Jian , Xin Ling , Fang Yanyan , Zhou Qiao , Guo Jinchen , Sun Yanqiu , Wang Fanfan 
Published: May 2023

Abstract
Objective. The goal of this research was to probe the mechanism of Huangqin Qingre Chubi(HQC) in the improvement of immune inflammation of Ankylosing spondylitis(AS).
Methods. Clinical data mining was implemented to assess the efficacy of HQC in AS therapy. The target network of HQC active ingredients for AS therapy was established by network pharmacology. The relationships between HQC active ingredients and major objectives of AS were forecasted by molecular docking technology. Finally, Cytological tests were performed for the evaluation of the effects of the drugs on the core targets.
Results. Clinical data mining indicated that HQC was highly related to the improvement of immunoinflammatory markers.Network pharmacology results demonstrated that the main targets of HQC, including Peroxisome proliferator-activated receptor gamma (PPARG),Prostaglandin G synthase 2(PTGS2), recombinant catalase(CAT), Chemokine (C-X-C motif) ligand 8(CXCL8), and Vascular endothelial growth factor(VEGFA), were closely associated with the signaling pathways of IL-17, NF-kappa B, TNF, T cell receptor, and Th1, and Th2 cell differentiation in AS therapy. In the molecular docking analysis of the HQC active components, 70% of the binding energy was under -4.25 kcal/mol, and 53% of the binding energy was no more than -5.0 kcal/mol, of which the binding energy of stigmasterol with PPARG, CXCL8, and CAT were under -7.50 kcal/mol, implying that the chemically active ingredients of HQC formula have good binding activity against inflammatory targets. In vitro cell experiment results suggested that HQC down-regulated the expression of PTGS2, and up-regulated the expression of PPARG and CAT.
Conclusion. HQC exerts functions in improving the immune inflammatory indicators in patients with AS for the reason that the active ingredient of Traditional Chinese medicine(TCM) in HQC act through oxygen species, oxidative stress, transcription factors, and apoptosis.
Suggestion the role of antibodies/immunoglobulins, produced by non-lymphoid cells, tissues and organs, as participants in different regulatory mechanisms
Authors: Sainova Iskra Ventseslavova , Kolyovska Vera , Ilieva Iliana , Drenska Desislava , Maslarov Dimitar 
Published: May 2023

Abstract
The main goal was related with a better understanding of the intra- and extra-cellular inter-molecular interactions in the different regulatory pathways, by which the separate cells, tissues and organs communicate to each other. The functions of tri-peptide Glutathione (GSH) as tumor suppressor, neuroprotector and endocrine regulatos, but also its role in the regulation of cellular growth, proliferation, differentiation and death, as well as of inflammatory and pro-inflammatory processes has been proved. In the current study, total extracts (controls) from rat brain and rat pancreas were prepared. Separate amounts were passed through GSH-agarose columns to select molecules, possessing affinity to GSH. The average titers of gangliosides and of anti-ganglioside antibodies in the so prepared biological samples were determined by enzyme-linked immunosorbent assay (ELISA). Possibility about production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs was suggested, probably as one of the steps in the regulatory pathways. Because the so produced antibodies are outside the germinative centers of the specialized lymphoid tissues and organs, control of their function by small ions and molecules as gangliosides is very important, by direct intra- and extra-cellular inter-molecular interactions or by indirect influence on various regulatory mechanisms.
A Novel Approach for Screening Standard Anteroposterior Pelvic Radiographs in Children
Authors: Sha Jia , Huang Luyu , Chen Yaopeng , Lin Jincong , Fan Zongzhi , Li Yi , Yan Yabo 
Published: May 2023

Abstract
Anteroposterior pelvic radiography is the first-line imaging modality for diagnosing developmental dysplasia of the hip (DDH). Nonstandard radiographs with pelvic malposition make the correct diagnosis of DDH challenging. However, as the only method available for screening standard pelvic radiographs, traditional manual assessment is relatively laborious and potentially erroneous. We retrospectively collected 3,247 pelvic radiographs. There were 2,887 radiographs randomly selected to train and optimize the AI model. Then 362 radiographs were used to test the model’s diagnostic performance. Its diagnostic accuracy was assessed using receiver operating characteristic (ROC) curves and measurement consistency using Bland-Altman plots. In 362 radiographs, the AI model’s area under ROC curves, accuracy, sensitivity, and specificity for quality assessment was 0.993, 99.4% (360/362), 98.6% (138/140), and 100.0% (220/220), respectively. Compared with clinicians, the 95% limits of agreement (Bland-Altman analysis) for pelvic tilt index (PTI) and pelvic rotation index (PRI), as determined by the model, were − 0.052–0.072 and − 0.088 − 0.055, respectively. Conclusions: The artificial intelligence-assisted method was more efficient and highly consistent with clinical experts. This method can be used for real-time validation of the quality of pelvic radiographs in current picture archiving and communications systems (PACS).
Influence of Plastic Aggregate Geometry on Strength Properties of Cement-Stabilized Macadam
Authors: Chi Mingxiang , Shen Zhenzong , Chen Shibin , Yao Yunshi , Shen Jianjun , Li Chao , Yan Qingyao 
Published: May 2023

Abstract
In order to improve the toughness and flexibility of cement-stabilized macadams and inhibit crack formation and propagation, the influence of plastic aggregate geometry on the strength properties of cement-stabilized macadam is studied. The surface roughness of plastic aggregate was designed into Ra 12.5 and Ra 25. When the content of plastic coarse aggregate was gradually increased from 4–8%, 12%, 16%, 20%, and 24%, the compressive strength, splitting strength, and the tensile-compression ratio of the cement-stabilized macadam made of aggregate with rough surface texture were found higher than those of aggregate with smooth surface texture. The rough cylindrical, spherical, spindle, and dumbbell-shaped plastic coarse aggregates were designed and prepared for testing. The test results showed the maximum compressive strength, splitting strength, and tensile-compression ratio of cement-stabilized macadam with the dumbbell-shaped plastic coarse aggregate. The tensile-compression ratio increased by 15.2% compared to natural aggregate cement-stabilized macadam. The results show that under the same conditions, the plastic aggregate with different geometry can improve the compressive and splitting strengths of cement-stabilized macadam and significantly improve the toughness and flexibility, with dumbbell-shaped plastic coarse aggregate having the greatest improvement, especially compared with natural aggregate cement-stabilized crushed stone. This research provides insights for improving pavement engineering quality and new ideas for utilizing waste plastics.
A Method for Selecting Optimal MRI Sequence for Automatic Segmentation Based on Edge Analysis
Authors: Jung Yunsub , Simonsen Morten Bilde , Andersen Michael Skipper 
Published: May 2023

AbstractIn segmentation research using magnetic resonance imaging (MRI) images, the sequence is an important factor affecting segmentation performance. Therefore, a selection method is required to find the best-suited sequence according to a segmentation target. This study presents a method for finding the most suitable MRI sequence for automatic segmentation. Based on profile information of virtual rays, we devised metrics to compute the edge sharpness and contrast. The analysis was proceeded using three sequences (T1: T1-weighted, PD: proton density-weighted, and SPGR: fat-suppressed 3D spoiled gradient-echo) from five defined edges (EBB: between cancellous bone and cortical bone, EBC: between cortical bone and cartilage, ECF: between cartilage and fat, ECM: between cartilage and meniscus, EBT: between cortical bone and tissue). The edge characteristics were compared in the three sequences using the proposed metrics, and the inter-subject variability was evaluated as well. In the case of sharpness, T1 showed the highest at the EBB, ECF, and EBT(p < .05). SPGR was the highest at the EBC, and PD was the highest at the ECM(p < .005). For contrast, T1 was the highest at the EBBand EBT(p < .05). SPGR was the highest at the ECF(p < .005), and PD was the highest at the ECM(p < .005). PD and SPGR had similar contrast values at the EBC(PD ≈ SPGR > T1). It was confirmed that the edge properties of the structure depend on the type of adjacent materials. The presented method showed consistent results according to the edge, and it was confirmed that new metrics were suitable for finding the most suitable sequence for segmentation. The method and metrics we present quantitatively evaluate the edge characteristics, which will be a useful way for finding the most suitable MRI sequence for segmentation study.
Aerobic exercise improves hepatic steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway
Authors: Wu Baoai , Tian Yiming , Xu Chong , Li Longpeng , Guan Yue , Chen Yinghua , Zhao Jinfeng 
Published: May 2023

Abstract
The role of aerobic exercise in preventing and ameliorating non-alcoholic fatty liver disease (NAFLD)has been widely demonstrated. MicroRNA-34a (MiR-34a) , a small non-coding RNA that regulates gene expression, has received much attention for its essential role in the progression of metabolic liver disease. However, it is unclear whether exercise can prevent and ameliorate hepatic lipid degeneration by targeting miR-34a and its underlying molecular mechanisms. In this study, normal or high-fat diet -induced male C57BL/6J mice underwent an 8-week running program (6 days/week, 18 m/min, 50 min, 6% incline) or remained sedentary. Histomorphometric examination and biochemical analysis were performed to evaluate intrahepatic lipid deposition. Adeno-associated viral vectors were injected into mice to construct miR-34a liver-specific overexpression mice. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the expression of genes and proteins related to lipid metabolism in the liver. After exercise intervention, the liver weight/body weight, liver TG, and HE of mice in the High-fat diet with aerobic exercise group (HCE) indicated significant improvement in hepatic steatosis, and mir-34a levels were significantly suppressed. This study identified aerobic exercise improves hepatic lipid degeneration by increasing the expression of its target genes PPARα and SIRT1 through mir-34a and activating the expression of AMPK and changes in genes related to lipid metabolism downstream of the PPARα/SIRT1-AMPK pathway.
A multi-approach perspective for the attention of the diversity of the audiences in science museums: the pedagogical model of the Bogota Planetarium.
Authors: Leal Maria Angelica , Molina Carlos , Valbuena Miguel , Guerra Yeimy , Carvajal Milton , Tovar David , Prada Nestor , Guevara Jorge , Ruiz Wyndy , Guerrero Camilo , Sepulveda Karina , Caicedo David , Benavides Juan Sebastian , Ceferino Maria Trinidad , Montenegro Oscar , Altafulla Jose Luis , Cuartas Kelly , Pulido Carolina , Cano Jeison Fabian , Cuervo Juan 
Published: May 2023

Abstract
The Bogota Planetarium, in its role as a science center, cultural equipment of the District Institute of the Arts IDARTES and science museum aims to ensure the exercise of cultural rights through the construction of communities with emphasis on the appropriation of scientific, artistic, and technological knowledge of the various audiences it impacts. For this purpose, an interdisciplinary network in dissemination and education has been proposed, implying a unified vision for developing actions. For this reason, it is necessary to have a pedagogical model for non-conventional educational spaces, which, for the Bogota Planetarium, is based on a constructivist theory that brings together the approaches of science, technology, and society, differential approach, universal learning design, an investigative approach, and visible thinking; these will be unified and put into action through a reflective methodology that works from a transdisciplinary thematic axis. Finally, based on the results and evidence obtained from the implementation of visible thinking, it will be possible to conduct a contextualized evaluation that will lead to feedback on the model and the processes.
Comparison of Self-Administered Web-Based and Printed Food Frequency Questionnaires for Dietary Assessment in the Iranian adult population
Authors: Morvaridi Mehrnaz , Karandish Majid , Bazyar Hadi , Rasekhi Hamid , Behbahani Hossein Bavi 
Published: May 2023

Abstract
Background: Today, with the increasing availability and usage of the internet, dietary assessment tools including FFQ are designed and modified using web technology. The web-based Nutripors food frequency questionnaire (FFQ) was developed as a dietary assessment tool for healthy adults in Iran.
Objective: The present study aimed to develop a web-based FFQ for the Iranian adult population to assess dietary intakes and compare it with the validated printed FFQ.
Methods: Eligible participants were randomly asked to complete both the printed FFQ and Web-Based FFQ. The type of FFQ was changed for each participant after ten days. The level of agreement between two FFQs was evaluated using the Bland and Altman method and correlations.
Results: Out of 190 participants, the final analysis was performed on 141 participants. The results are from the dietary assessment of 70 females (49.6%) and 71 males (50.4%) that completed both FFQs. The level of agreement was high for all nutrient intakes (r>0.8). In food groups, “Whole grains” has the highest (0.84), and “Potato” has the lowest (0.25) correlation coefficients. Also, there were no significant differences in the time spent to complete the questionnaires.
Conclusion: We concluded that Web-Based FFQ as an online FFQ has good agreement with the validated printed FFQ and properly reflects the nutrient and food group intakes.
Combination therapy - is it time to start using? - A case-based review
Authors: Ferrari Luis Fernando Fernandes , Franco Andre S. , Saad Carla Gonçalves Schahin , de Moraes Julio César Bertacini 
Published: May 2023

Abstract
Background
This case-based review presents a Reactive arthritis (ReA) refractory to conventional therapies with excellent response to the combination of ustekinumab and tofacitinib. This is the first case report of ReA with combination therapy with a bDMARD and a tsDMARD. Although not a consensus, combination therapy is already used especially in psoriasis, psoriatic arthritis and inflammatory bowel diseases, motivating this choice since in monotherapy the last two drugs achieved the best responses in different domains, justifying the possibility of blocking several pathophysiological mechanisms of immune-mediated arthritis.
Case presentation:
A man with peripheral and axial arthritis, conjunctivitis, balanoposthitis, keratoderma blenorrhagicum and urethral discharge preceded by diarrhea, treated with antibiotics, then methotrexate and prednisone were introduced for ReA. Along the years he received three different anti-TNF drugs with no response. Same as IL-17a monoclonal antibody with primary failure. Treatment was changed to ustekinumab with resolution of cutaneous involvement, but not articular. Then, tofacitinib was tried and arthritis improved but skin relapsed. Since severity and refractoriness of the case, combination of ustekinumab and tofacitinib was considered, achieving remission in both domains
Conclusions
ReA has a difficult diagnosis and treatment, anti-TNF, anti-IL 17, anti-IL 12/23, and JAK inhibitors may be used based on limited data and clinical experience, specially adapted from other spondyloarthritis. This case had poor disease control with all such bDMARD used separately, but achieved low disease activity and resolution of skin lesions with combination of JAK inhibitor and anti-IL 12/23 with no severe adverse effects during follow-up.