bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring of SARS‐CoV‐2 RNA in wastewater: A surveillance tool to foresee infection's evolution in the Mexican Caribbean
Authors: Hernández‐Terrones Laura Margarita , Carrillo‐Reyes Julián , Ayala‐Godoy Jairo A. , Guerrero‐Ruiz Eugenio , Vargas Leslie Meredith García , Prado‐Guevara Bruno Abraham , Rodríguez‐Abraham Melissa Fernanda , Buitrón Germán 
Published: April 2023

AbstractThe study aims to determine SARS‐CoV‐2 RNA in sewage of Cancun wastewater treatment plants, the main touristic destination of Mexico, and to estimate the infected persons during the sampling period. SARS‐CoV‐2 RNA traces were detected in the inlet of the five plants during almost all the sampling months. However, there is no presence of SARS‐CoV‐2 RNA traces in the effluent of the five WWTPs during the study period. ANOVA analysis showed differences in the concentrations of RNA traces of SARS‐CoV‐2 between the sample dates, but no differences were found from one WWTP to another. Estimated infected individuals by Markov chain Monte Carlo simulation are higher (between 77% and 91%) than the cases reported by the health authority. Wastewater monitoring and the estimation of infected individuals are a helpful tool, because estimation provides early warning signs on how broadly SARS‐CoV‐2 is circulating in the city, and led to the authorities to take measures wisely.Practitioner Points

There is no presence of SARS‐CoV‐2 RNA traces in the effluent of the facilities, suggesting the effectiveness of treatment.
Surveillance of viral RNA concentrations at treatment plants revealed presence in the influent of five plants
Estimated infected individuals by MCMC simulation are higher than cases reported by health authority
Environmental surveillance approach in wastewater influent is helpful to identify the clusters and to take informed decisions
Injectable platelet-rich fibrin with demineralized freeze-dried bone allograft compared to demineralized freeze-dried bone allograft in intrabony defects of patients with stage-III periodontitis: a randomized controlled clinical trial
Authors: Alshoiby Mashaal Mohammed , Fawzy El-Sayed Karim Mohamed , Elbattawy Weam , Hosny Manal Mohamed 
Published: April 2023

Abstract
Aim
The current randomized controlled clinical trial assessed the effect of injectable platelet-rich fibrin (I-PRF) combined with demineralized freeze-dried bone allograft (DFDBA) compared to DFDBA alone in the management of intrabony defects of stage-III periodontitis patients.

Methodology
Following sample size calculation, twenty stage-III periodontitis patients with ≥ 5 mm clinical attachment level (CAL)-loss and ≥ 3 mm intrabony defects were randomized into test (I-PRF + DFDBA; n = 10) and control (DFDBA; n = 10) groups. CAL (primary outcome), periodontal probing depth (PPD), gingival recession depth (GRD), full-mouth plaque scores (FMPS), full-mouth bleeding scores (FMBS), radiographic linear defect depth (RLDD), and bone fill (secondary outcomes) were examined at baseline, 3, 6, and 9 months post-surgically.

Results
I-PRF + DFDBA and DFDBA independently demonstrated significant intragroup CAL-gain, PPD-, and RLDD-reduction at 3, 6, and 9 months (p < 0.05), with no significant intergroup differences observed (p > 0.05). CAL-gain (mean ± SD) of 2.40 ± 0.70 mm and 2.50 ± 0.85 mm and PPD-reduction of 3.50 ± 1.18 mm and 2.80 ± 0.42 mm were demonstrated for I-PRF + DFDBA and DFDBA at 9 months respectively. Both groups showed significant intragroup RLDD improvement, with a RLDD of 3.58 ± 0.66 mm and 3.89 ± 1.57 mm for I-PRF + DFDBA and DFDBA at 9 months respectively. Stepwise linear regression analysis revealed that baseline RLDD and bone fill at 9 months were significant predictors of CAL (p < 0.05).

Conclusion
Within the present study’s limitations, DFDBA with or without I-PRF resulted in significant improvement in clinical and radiographic periodontal parameters in the surgical treatment of periodontal intrabony defects of stage-III periodontitis patients. Addition of I-PRF to DFDBA does not appear to significantly enhance the DFDBA’s reparative/regenerative outcomes.

Clinical relevance
Within the current study’s limitations, routinely adding I-PRF to DFDBA cannot be recommended to significantly improve DFDBA’s treatment outcomes in intrabony defects.
Synthesis of porous C/Fe3O4 microspheres by spray pyrolysis with NaNO3 additive for lithium-ion battery applications
Authors: Adi Akylbek , Taniguchi Izumi 
Published: April 2023

AbstractIn this work, we successfully synthesized porous C/Fe3O4 microspheres by spray pyrolysis at 700ºC with a sodium nitrate (NaNO3) additive in the precursor solution. Furthermore, we studied their electrochemical properties as anode material for Li-ion batteries. The systematic studies by various characterization techniques show that NaNO3 catalyzes the carbonization of sucrose and enhances the crystallization of Fe3O4. Moreover, an aqueous etching can easily remove sodium compounds to produce porous C/Fe3O4 microspheres with large surface areas and pore volumes. The porous C/Fe3O4 microspheres exhibit a reversible capacity of ~780 mAh g–1 in the initial cycles and ~520 mAh g–1 after 30 cycles at a current density of 50 mA g–1. Moreover, a reversible capacity of ~400 mAh g–1 is attainable after 200 cycles, even at a high current density of 500 mA g–1. The wide range of pores produced from the removal of sodium compounds might enable easy electrolyte penetration and facilitate fast Li-ion diffusion, while the N-doping can promote the electronic conductivity of the carbon. These features of porous C/Fe3O4 microspheres led to the improved electrochemical properties of this sample.
Graphical Abstract
Metabolomics of early myocardial ischemia
Authors: Hasselbalch Rasmus Bo , Kristensen Jonas Henrik , Strandkjær Nina , Jørgensen Nicoline , Bundgaard Henning , Malmendal Anders , Iversen Kasper Karmark 
Published: April 2023

Abstract
Introduction
Diagnosing myocardial infarction is difficult during the initial phase. As, acute myocardial ischemia is associated with changes in metabolic pathways, metabolomics may provide ways of identifying early stages of ischemia. We investigated the changes in metabolites after induced ischemia in humans using nuclear magnetic resonance spectroscopy (NMR).

Methods
We included patients undergoing elective coronary angiography showing normal coronary arteries. These were randomized into 4 groups and underwent coronary artery occlusion for 0, 30, 60 or 90 s. Blood was collected over the next 3 h and analyzed using NMR. We used 2-way ANOVA of time from baseline- and treatment group to find metabolites that changed significantly following the intervention and principal component analysis (PCA) to investigate changes between the 90 s ischemia- and control groups at 15 and 60 min after intervention.

Results
We included 34 patients. The most pronounced changes were observed in the lipid metabolism where 38 of 112 lipoprotein parameters (34%) showed a significant difference between the patients exposed to ischemia and the control group. There was a decrease in total plasma triglycerides over the first hour followed by a normalization. The principal component analysis showed a effects of the treatment after just 15 min. These effects were dominated by changes in high-density lipoprotein. An increase in lactic acid levels was detected surprisingly late, 1–2 h after the ischemia.

Conclusion
We investigated the earliest changes in metabolites of patients undergoing brief myocardial ischemia and found that ischemia led to changes throughout the lipid metabolism as early as 15 min post-intervention.
Seasonal and Morphological Controls on Nitrate Retention in Arctic Deltas
Authors: Knights Deon , Piliouras Anastasia , Schwenk Jon , Hariharan Jayaram , Russoniello Christopher 
Published: April 2023

AbstractEstimates of nitrate loading to the Arctic Ocean are limited by the lack of field observations within deltas partly due to logistical constraints. To overcome this limitation, we use a remote sensing framework to estimate retention of nitrate in Arctic deltas. We achieve this by coupling hydrological and biogeochemical process models at the network scale for five major Arctic deltas. Binary masks of delta channels were used to simulate flow direction and magnitude through networks. Models were parameterized using historical and seasonal observations. Simulated nitrate retention ranged from 2.9% to 15% of the incoming load. Retention rates were largest during winter but smallest during spring conditions when increased discharges export large nitrate masses to the coast. Under future climate scenarios, retention rates fall by ∼1%–10%. Arctic deltas have an important effect on the magnitude of nitrate entering Arctic seas and the inclusion of processing in deltas can improve flux estimates.
Preparatory Slip in Laboratory Faults: Effects of Roughness and Load Point Velocity
Authors: Guérin‐Marthe Simon , Kwiatek Grzegorz , Wang Lei , Bonnelye Audrey , Martínez‐Garzón Patricia , Dresen Georg 
Published: April 2023

AbstractAseismic slip may occur during a long preparatory phase preceding earthquakes, and what controls it remains poorly understood. In this study, we explored the role of load point velocity and surface roughness on slow slip during the preparatory stage prior to stick‐slip events. To that end, we conducted displacement‐rate controlled friction experiments by imposing varying load point velocities on sawcut granite samples with different surface roughness at a confining pressure of 35 MPa. We measured the average slip along the fault with the recorded far‐field displacements and strain changes, while acoustic emission sensors and local strain gages were used to capture local slip variations. We found that the average amount of aseismic slip during the preparatory stage increases with roughness, whereas precursory slip duration decreases with increased load point velocity. These results reveal a complex slip pattern on rough faults which leads to dynamic ruptures at high load point velocities.
Collaborative stochastic expansion planning of cyber‐physical system considering extreme scenarios
Authors: Zhang Yiwei , Li Chengze , Wan Haiyang , Shi Qingxin , Liu Wenxia , Xu Yanhui 
Published: April 2023

AbstractIn this paper, a collaborative stochastic expansion planning model of cyber‐physical system (CPS) with resilience constraints is proposed. The model can reduce the coupling risk and enhance the resilience under extreme scenarios, from the perspective of the structural/functional coupling between the physical and cyber systems. The model is to collaboratively optimize expansion transmission line siting, expansion communication fibre siting, and service routing distribution to minimize total investment cost. Constraints are divided into conventional constraints and resilience constraints. In the resilience constraints, the impact of cyber failures on the dispatch strategy is analyzed, and the load shedding in stochastic scenarios is limited to guarantee the resilience of the planning scheme. Particularly, to reach a stable solution for the stochastic planning, a mixed scenario set is proposed based on the probabilistic typhoon model and the complex network theory. Finally, the model is solved by the progressive hedging (PH) algorithm. The case studies of the IEEE RTS‐79 test system demonstrate that, compared with the traditional independent planning model, the model proposed in this paper performs better in limiting load loss and enhancing resilience under extreme scenarios.
Withdrawing Assisted Nutrition in Neonates: A Survey on Attitudes and Insights in Argentina
Authors: Mariani Gonzalo L. , Konikoff Laura L. 
Published: April 2023

Objective This study aimed to assess beliefs and attitudes of Argentinean neonatologists and neonatal nurses regarding end-of-life care of newborn infants, including withdrawal of clinically assisted nutrition and hydration (CANH).
Study Design A five-domain survey was sent to 465 neonatal health care workers, which included demographic data, general ethical concepts, participation in end-of-life decisions, beliefs about end-of-life care practices, and presentation of four clinical scenarios. Standard statistical tests were used, and a multivariable analysis was done to evaluate variables independently associated with rejecting the withdrawal of CANH.
Results A total of 227 questionnaires were anonymously completed, 60% by physicians and 40% by nurses. More respondents agreed to withdraw mechanical ventilation in comparison to CANH in patients under certain circumstances (88 vs. 62%, p < 0.01). The most accepted variables when deciding to withdraw care were the quality of life perceived by parents (86%) and their religious beliefs (73%). A total of 93% agreed that parents should be included in the decision, but only 74% acknowledged they are included in real practice. Considering the scenario of a newborn with severe and irreversible neurological compromise, 46% of respondents were opposed to suspending enteral nutrition. No independent variables were found to be associated with opposing the withdrawal of CANH. Of those agreeing to withdraw enteral feeds under certain circumstances, for the severely neurologically affected neonate 58% would either refuse to limit enteral feeds or consult with an ethics committee before doing so. When given the scenario of their own severe and irreversible neurological compromise, 68% agreed to have enteral feeds withdrawn to themselves, and they were more likely to agree with withdrawing feeds for the severely compromised neonate (odds ratio: 7.2; 95% confidence interval: 2.7–24.1).
Conclusion While most health care providers agreed to withdraw life-sustaining support under certain conditions, many were reluctant to suspend CANH. Many responses differed when being asked as general statements versus actual clinical scenarios.
Key Points
Predictors of Metformin Side Effects in Patients with Newly Diagnosed Type 2 Diabetes Mellitus
Authors: Alibrahim Nassar Taha Yaseen , Chasib Mohammed Ghazi , Hamadi Saad Shaheen , Mansour Abbas Ali 
Published: April 2023

Abstract
Introduction Metformin has become the first-line agent for the treatment of type 2 diabetes mellitus (T2DM) in several international guidelines. Up to 25% of patients suffer from gastrointestinal side-effects, with approximately 5% unable to tolerate metformin at all.
Objective We aimed to study the effect of variables that may influence the development of metformin side effects and/or intolerance.
Method A prospective study was conducted from April 1, 2021 to March 30, 2022. One-hundred and forty-eight patients newly diagnosed with T2DM were enrolled in the study, and divided into two groups—those who were escalate to the maximum dose of metformin over 2 weeks (n = 43) and the other group over 4 weeks (n = 105). We studied the variables that may affect the development of side effects including age, gender, body mass index (BMI), lipid profile, glycemic level, and the use of other antidiabetic medications besides the duration of dose escalation.
Results Total number of patients who developed side effects was 59 (39.9%). Twenty-four (55.8%) and 35 (33.3%) patients were put in the rapid and slow escalation groups, respectively. Twenty-six (17.6%) patients developed diarrhea that was the most common side effect. Two (2.7%) men and ten women (13.5%) had stopped metformin due to severe side effects developed after initiation (p = 0.016). The mean BMI for the patients who discontinued metformin was 34.7 ± 4.1 kg/m2 in the rapid escalation arm and 31.6 ± 3.3 kg/m2 in the slow escalation arm (p = 0.003). The mean of fasting blood glucose for the patients who discontinued metformin in the rapid and slow escalation arms was 200.6 ± 25.6 and 173.4 ± 36.5 mg/dL, respectively (p = 0.022).
Conclusion The severity of metformin side effects is higher in women than in men, making more women to discontinue the drug. Besides, a higher fasting blood sugar and BMI are associated with a higher rate of discontinuation. A rapid dose escalation is associated with a higher frequency of side effects. Diarrhea is the commonest side effect encountered.
Epidemiology of porocarcinoma in England 2013–2018: a population-based registry study
Authors: Joshy Jilse , van Bodegraven Birgitta , Mistry Khaylen , Craig Paul , Rajan Neil , Vernon Sally , Levell Nick J , Venables Zoe C 
Published: April 2023

Abstract

Background
Porocarcinoma (PC) is a cutaneous malignancy that differentiates towards (possibly arises from) the sweat ducts and glands. Lack of histological diagnostic markers makes clinical and pathological diagnosis complex. The limited data available suggest the incidence is increasing; however, this remains to be established in national epidemiological studies.

Objectives
To report the incidence, treatment and survival of patients with PC in England from 1 January 2013 to 31 December 2018 using national cancer registry data.

Methods
PC diagnoses in England during 2013–2018 were identified from the National Disease Registration Service using morphology and behaviour codes. These were registered from routinely collected pathology reports and cancer outcomes and services datasets. The 2013 European age standardized incidence rates (EASRs), Kaplan–Meier all-cause survival and log-rank test were calculated.

Results
In total, 738 tumours (396 in males and 342 in females) were diagnosed. The median age at diagnosis was 82 years old (interquartile range 74–88). The most frequently affected site were lower limbs (35.4%), followed by the face (16%). The majority of the cohort received surgical excision (73.0%). The Kaplan–Meier all-cause survival was 45.4% at 5 years, which was lower than in previous studies. The EASR for the whole population was 0.25 [95% confidence interval (CI) 0.23–0.27] per 100 000 person-years (PY)]. PC incidence rates in the East of England (EASR of 0.54, 95% CI 0.47–0.63 per 100 000 PY) were three times higher than the South West (EASR of 0.14, 95% CI 0.10–0.19 per 100 000 PY) where the regional rates were the lowest.

Conclusions
This study shows that there is large variation in the EASRs of PC across England. This may reflect differences in how PC is diagnosed and registered in different regions in England. These data support national assessment of the management of PC, which will inform future studies and guideline development.
Clinical and immunological characteristics of patients with combined anti-glomerular basement membrane disease and IgA nephropathy
Authors: Shen Cong-rong , Jia Xiao-yu , Cui Zhao , Yu Xiao-juan , Zhao Ming-hui 
Published: April 2023

ABSTRACT

Background
The combination of anti–glomerular basement membrane (GBM) disease and immunoglobulin A nephropathy (IgAN) has been well documented in sporadic cases, but lacks overall assessment in large collections. Herein, we investigated the clinical and immunological characteristics and outcome of this entity.

Methods
Seventy-five consecutive patients with biopsy-proven anti-GBM disease from March 2012 to March 2020 were screened. Among them, patients with concurrent IgAN were identified and enrolled. The control group included biopsied classical anti-GBM patients during the same period, excluding patients with IgAN, other glomerular diseases or tumors, or patients with unavailable blood samples and missing data. Serum IgG and IgA autoantibodies against GBM were detected by enzyme-linked immunosorbent assay, as were circulating IgG subclasses against GBM.

Results
Fifteen patients with combined anti-GBM disease and IgAN were identified, accounting for 20% (15/75) of all patients. Among them, nine were male and six were female, with an average (± standard deviation) age of 46.7 ± 17.3 years. Thirty patients with classical anti-GBM disease were enrolled as controls, with 10 males and 20 females at an average age of 45.4 ± 15.3 years. Patients with combined anti-GBM disease and IgAN had restricted kidney involvement without pulmonary hemorrhage. Compared with classical patients, anti-GBM patients with IgAN presented with significantly lower levels of serum creatinine on diagnosis (6.2 ± 2.9 vs 9.5 ± 5.4 mg/dL, P = .03) and less occurrence of oliguria/anuria (20%, 3/15 vs 57%, 17/30, P = .02), but more urine protein excretion [2.37 (1.48, 5.63) vs 1.11 (0.63, 3.90) g/24 h, P = .01]. They showed better kidney outcome during follow-up (ESKD: 47%, 7/15 vs 80%, 24/30, P = .03). The autoantigen and epitope spectrum were comparable between the two groups, but the prevalence of circulating anti-α3(IV)NC1 IgG1 (67% vs 97%, P = .01) and IgG3 (67% vs 97%, P = .01) were lower in patients with IgAN.

Conclusions
Concurrent IgAN was not rare in anti-GBM disease. Patients showed milder kidney lesions and better recovery after immunosuppressive therapies. This might be partly explained by lower prevalence of anti-GBM IgG1 and IgG3 in these patients.
Whole genome assemblies of Zophobas morio and Tenebrio molitor
Authors: Kaur Sabhjeet , Stinson Sydnie A , diCenzo George C 
Published: April 2023

Abstract
Zophobas morio (=Zophobas atratus) and Tenebrio molitor are darkling beetles with industrial importance due to their use as feeder insects and their apparent ability to biodegrade plastics. High quality genome assemblies were recently reported for both species. Here, we report additional independent Z. morio and T. molitor genome assemblies generated from Nanopore and Illumina data. Following scaffolding against the published genomes, haploid assemblies of 462 Mb (scaffold N90 of 16.8 Mb) and 258 Mb (scaffold N90 of 5.9 Mb) were produced for Z. morio and T. molitor, respectively. Gene prediction led to the prediction of 28,544 and 19,830 genes for Z. morio and T. molitor, respectively. Benchmarking Universal Single Copy Orthologs (BUSCO) analyses suggested that both assemblies have a high level of completeness; 91.5 and 89.0% of the BUSCO endopterygota marker genes were complete in the Z. morio assembly and proteome, respectively, while 99.1 and 92.8% were complete in the T. molitor assembly and proteome, respectively. Phylogenomic analyses of four genera from the family Tenebrionidae yielded phylogenies consistent with those previously constructed based on mitochondrial genomes. Synteny analyses revealed large stretches of macrosynteny across the family Tenebrionidae, as well as numerous within-chromosome rearrangements. Finally, orthogroup analysis identified ∼28,000 gene families across the family Tenebrionidae, of which 8,185 were identified in all five of the analyzed species, and 10,837 were conserved between Z. morio and T. molitor. We expect that the availability of multiple whole genome sequences for Z. morio and T. molitor will facilitate population genetics studies to identify genetic variation associated with industrially relevant phenotypes.