bluuebunny/crossref_metadata_2025_split huggingface dataset:
An assumption of in situ resource utilization for “bio-bricks” in space exploration
Authors: Zuo Hongyan , Ni Shuisong , Xu Meiying 
Published: April 2023

Microbially induced carbonate precipitation (MICP) mimics the natural cementation process that occurs in various geological settings by using the bicarbonate minerals resulting from various bacterial metabolic pathways as cementing agents. This bio-technique can be used to manufacture so-called “bio-bricks,” which rival regular bricks in strength and durability. In the last two decades, MICP has been increasingly utilized for the maintenance and repair of infrastructure. More recently, this process has also been shown to have great potential as an energy-saving and cost-effective means of in situ resource utilization (ISUR) to produce construction materials; these can be utilized for extraterrestrial human settlements for space programs such as lunar exploration. We thus review the description of natural cementation, the anaerobic and aerobic bacterial metabolic activities leading to calcium carbonate precipitation, the properties of the lunar regolith, the production of bio-bricks, and potential research needs.
Porous single crystalline-like titanium dioxide monolith with enhanced photoelectrochemical performance
Authors: Liu Kaipeng , Cheng Fangyuan , Luo Yunfei , Liu Ling , Wang Changtao , Xie Kui , Luo Xiangang 
Published: April 2023

Macro-sized porous single crystalline-like (PSC-like) TiO2 is endowed with unique structural advantages due to its structural consistency and porosity in a large area, which would significantly enhance its photoelectrochemical function. However, there are significant technical challenges in the growth of porous single crystalline-like monoliths. The consistency of structure dominates the structure so that the grain boundary is reduced to the minimum, which is in contradiction with the three-dimensional percolation structure. Here we report a lattice reconstruction strategy based on solid-solid transformation to grow porous single crystal-like anatase TiO2 dominated by (200) and (101) facets at 2 cm scale. In comparison with the traditional definition of porous single crystal, it has two different lattice orientations, but still has good photoelectrochemical properties. The band gap engineering introduces Ti3+ gap into the lattice to generate TinO2n−1 with Magneli phase, limiting the created active structure to the lattice with two-dimensional surface, which would open a new avenue to create highly active surfaces to capture photons and transport electrons stably. The PSC-like TinO2n−1 provides enhanced exciton lifetime (3–5 ns) as a photocatalytic catalyst and shows significant visible light absorption. The independent PSC-like TinO2n−1 delivers high photocurrent of 1.8–5.5 mA · cm−2 at room temperature and does not decay for 10 h.
GutAlive® enables DNA-based microbiome analysis without disrupting the original composition and diversity
Authors: Montero Ignacio , Barrientos Desirée , Hidalgo-Cantabrana Claudio , Martínez-Álvarez Noelia 
Published: April 2023

IntroductionA precise fecal microbiome analysis requires normalized methods for microbiome sampling, transport and manipulation in order to obtain a representative snapshot of the microbial community. GutAlive® is the unique stool collection kit that generates an anaerobic atmosphere enabling oxygen sensitive bacteria to survive, maintaining the original microbiome composition and diversity.MethodsFive stool samples from different donors were collected using two different sampling devices, GutAlive® and Zymo DNA/RNA Shield®, and processed at four different time points. Shotgun metagenomics was used to evaluate the influence of the device and the processing timing on the microbial populations to unravel the potential fluctuations on the composition and diversity of the fecal microbiome and the metabolic pathways profiling. Additionally, RT-qPCR was used to quantify bacterial cell viability for downstream applications of microbiota samples beyond metagenomics.ResultsOur results show that GutAlive® enables bacterial cell viability overtime preserving DNA integrity, obtaining high-quantity and high-quality DNA to perform microbiome analysis using shotgun metagenomics. Based on the taxonomic profiling, metabolic pathways analysis, phylogeny and metagenome-assembled genomes, GutAlive® displayed greater performance without significant variability over time, showcasing the stabilization of the microbiome preserving the original composition and diversity. Indeed, this DNA stabilization is enabled with the preservation of bacterial viability on an anaerobic environment inside of the sampling device, without the addition of any reagents that interact directly with sample.ConclusionAll the above makes GutAlive® an user-friendly kit for self-collection of biological samples, suitable for microbiome analysis, diagnostics, fecal microbiota transplant and bacterial isolation, maintaining the stability and bacterial viability over time, preserving the original composition and diversity of the microbiome.
Experimental study on the repair of peripheral nerve injuries via simultaneously coapting the proximal and distal ends of peripheral nerves to the side of nearby intact nerves
Authors: Li Dongdong , Yang Qi , Liu Xin , Jia Jing , Liu Guangbo , Bai Kewen , Jia Shicheng , Peng Jun , Yu Fei 
Published: April 2023

IntroductionPeripheral nerve defect is a difficult disease to treat in clinical practice. End-to-side anastomosis is a useful method to treat it. At present, the end-to-side anastomosis method does not involve the proximal nerve, which results in a waste of proximal donor nerves, and even the formation of traumatic neuromas at the proximal end. The patients suffer from traumatic neuralgia and the curative effect is unsatisfactory.MethodsIn this study, an improved end-to-side anastomosis technique was proposed in this study: both the proximal and distal ends of the damaged common peroneal nerve were sutured to an adjacent normal tibial nerve. Moreover, the possible role and mechanism of the proposed technique were explained at the physiological and anatomical levels. In this study, a 10 mm common peroneal nerve defect was made in SD rats, and the rats were randomly divided into three groups. In Group I, the distal end of the common peroneal nerve was attached end-to-side to the fenestrated tibial nerve adventitia, and the proximal end was ligated and fixed in the nearby muscle. In Group II, the tibial nerve adventitia was fenestrated and the epineurial end-to-end anastomosis surgery was performed to suture the proximal and distal ends of the common peroneal nerve. Rats in Group III were taken as control and received sham operation. Twelve weeks after the operation, the recovery of the repaired nerve and distal effector functions were examined by the sciatic functional index, electrophysiology, osmic acid staining, the muscle wet weight ratio, and the muscle fiber cross-sectional area.ResultsIt was found that these results in Group II were similar to those in Group III, but better than those in Group I. Through retrograde tracing of neurons and Electrophysiological examination in Group II, the study also found that the proximal common peroneal nerve also could establish a connection with tibialis anterior, even gastrocnemius.DiscussionTherefore, it is inferred that fostering both the proximal and distal ends of defective peripheral nerves on normal peripheral nerves using the end-to-side anastomosis technique is a more effective approach to repairing injured nerves.
Research advances and trends in the surgical treatment of carpal tunnel syndrome from 2003 to 2022: A CiteSpace-based bibliometric analysis
Authors: Zheng Daqiang , Wu Zhiming , Li Lu , Chen Sichao , Chang Jianjun 
Published: April 2023

BackgroundCarpal Tunnel Syndrome (CTS) is one of the most common peripheral neuropathies. The typical symptoms are tingling and numbness in the median nerve distribution of the hand. Current treatment for CTS includes general conservative treatment and surgical treatment. Surgical treatment plays a crucial role in the management of CTS, but little bibliometric analysis has been conducted on it. Therefore, this study aimed to map the literature co-citation network using CiteSpace (6.1 R4) software. Research frontiers and trends were identified by retrieving subject headings with significant changing word frequency trends, which can be used to predict future research advances in the surgical treatment of CTS.MethodsPublications on the surgical treatment of CTS in the Web of Science database were collected between 2003 and 2022. CiteSpace software was applied to visualize and analyze publications, countries, institutions, journals, authors, references, and keywords.ResultsA total of 336 articles were collected, with the USA being the major publishing power in all countries/regions. JOURNAL OF HAND SURGERY AMERICAN VOLUME was the journal with the most published and co-cited articles. Based on keyword and reference co-citation analysis, keywords such as CTS, surgery, release, median nerve, and diagnosis were the focus of the study.ConclusionThe results of this bibliometric study provide clinical research advances and trends in the surgical treatment of patients with CTS over the past 20 years, which may help researchers to identify hot topics and explore new directions for future research in the field.
Lacosamide as the first add-on therapy in adult patients with focal epilepsy: A multicenter real-world study
Authors: Liu Wenyu , Li Wenjing , Wang Peiyu , Zhang Hesheng , Zhang Enhui , Wu Xintong , Zhou Dong 
Published: April 2023

BackgroundProspective observations on the effectiveness, safety, tolerance, and influence of comorbidity of add-on lacosamide (LCM) therapy are still lacking, especially for domestic generic LCM in China.ObjectiveIn this multicenter real-world study, we aimed to evaluate lacosamide (LCM) as the first add-on therapy in adult Chinese patients with focal epilepsy that had initially been treated with monotherapy.MethodsA cohort of consecutive focal epilepsy patients aged over 16 years were enrolled and followed at the multi-epilepsy centers in China. LCM was prescribed as the first add-on therapy. The main outcome measures included seizure frequency and response rate. Data on seizure-free rate, retention rate, scales of depression and anxiety, and adverse events were also collected as additional outcome measures.ResultsA total number of 107 adult subjects (60 men, 56.07%) were enrolled. The mean age was 37.16 ± 15.01 years, and the mean age at seizure onset was 312.35 ± 199.97 months. After the LCM add-on therapy, the ≥50% response rates were 76.19, 81.73, 94.12, and 95.79% at the visit at 4 weeks (visit 2), 8 weeks (visit 3), 16 weeks (visit 4), and 24 weeks (visit 5), respectively, compared to the baseline (visit 1). A total of 34 patients (31.78%) had no seizures during the whole follow-up period. The posttreatment emotional performance of the 97 subjects, defined as generalized anxiety disorder (GAD) and Neurological Disorders Depression Inventory (NDDI) scores, was significantly better than the baseline one. Only one patient suffered from mild dizziness.ConclusionLCM as the first add-on therapy in adult focal epilepsy in China was effective and safe. Further prospective studies with long-term follow-up periods are needed to confirm our present findings.Clinical trial registrationhttp://www.chictr.org.cn, ChiCTR2100042485.
Neuroprotective effects of resistance physical exercise on the APP/PS1 mouse model of Alzheimer’s disease
Authors: Campos Henrique Correia , Ribeiro Deidiane Elisa , Hashiguchi Debora , Glaser Talita , Milanis Milena da Silva , Gimenes Christiane , Suchecki Deborah , Arida Ricardo Mario , Ulrich Henning , Monteiro Longo Beatriz 
Published: April 2023

IntroductionPhysical exercise has beneficial effects by providing neuroprotective and anti-inflammatory responses to AD. Most studies, however, have been conducted with aerobic exercises, and few have investigated the effects of other modalities that also show positive effects on AD, such as resistance exercise (RE). In addition to its benefits in developing muscle strength, balance and muscular endurance favoring improvements in the quality of life of the elderly, RE reduces amyloid load and local inflammation, promotes memory and cognitive improvements, and protects the cortex and hippocampus from the degeneration that occurs in AD. Similar to AD patients, double-transgenic APPswe/PS1dE9 (APP/PS1) mice exhibit Αβ plaques in the cortex and hippocampus, hyperlocomotion, memory deficits, and exacerbated inflammatory response. Therefore, the aim of this study was to investigate the effects of 4 weeks of RE intermittent training on the prevention and recovery from these AD-related neuropathological conditions in APP/PS1 mice.MethodsFor this purpose, 6-7-month-old male APP/PS1 transgenic mice and their littermates, negative for the mutations (CTRL), were distributed into three groups: CTRL, APP/PS1, APP/PS1+RE. RE training lasted four weeks and, at the end of the program, the animals were tested in the open field test for locomotor activity and in the object recognition test for recognition memory evaluation. The brains were collected for immunohistochemical analysis of Aβ plaques and microglia, and blood was collected for plasma corticosterone by ELISA assay.ResultsAPP/PS1 transgenic sedentary mice showed increased hippocampal Aβ plaques and higher plasma corticosterone levels, as well as hyperlocomotion and reduced central crossings in the open field test, compared to APP/PS1 exercised and control animals. The intermittent program of RE was able to recover the behavioral, corticosterone and Aβ alterations to the CTRL levels. In addition, the RE protocol increased the number of microglial cells in the hippocampus of APP/PS1 mice. Despite these alterations, no memory impairment was observed in APP/PS1 mice in the novel object recognition test.DiscussionAltogether, the present results suggest that RE plays a role in alleviating AD symptoms, and highlight the beneficial effects of RE training as a complementary treatment for AD.
Knowledgeability about organic food consumption and the factors behind it
Authors: Barua Sukanya , Satyapriya  , Kumar Rajeev , Sangeetha V. , Muralikrishan L. , Wason Monika 
Published: April 2023

IntroductionThe popularity of organic food is in increasing trend due to the increased existence of synthetic food worldwide. Hence it is very important to know the knowledge level of city dwellers regarding various aspects of organic food and factors associated with their awareness level.ObjectivesThe primary objective of this research study is to develop a standardized knowledge test to assess the knowledge level of respondents regarding organic food and find out the factors behind elevated knowledge levels.MethodsA standardized knowledge test was developed comprising 26 knowledge items and pilot tested on 42 individuals. Difficulty index, discrimination index and point biserial correlation coefficient were calculated; only 21 knowledge statements were selected out of 26. The reliability coefficient and validity were checked and found satisfactory. The final knowledge test containing 21 knowledge statements was administered to 1050 respondents from various locations of the National Capital Region (NCR)-Delhi, India. After getting the knowledge score from each individual, it was classified as very low, low, medium, high and very high knowledge level. For determining factors contributing towards enhanced knowledge level, the correlation coefficient was calculated between independent socio-economic variables of each individual and their corresponding knowledge score. Regression analysis was also performed and developed a model to depict a relationship between the dependent variable i.e. knowledge level and independent variables.ResultsStandardized knowledge test depicted that a major portion of respondents (62.0%) possessed very low, low and medium levels of knowledge, whereas 23.5 and 14.5% of respondents had high and very high levels of knowledge regarding organic food. Independent variables like gender, education, family size, family income, internet, mass media exposure and social participation had a positive relationship with the knowledge level of respondents. The results of regression analysis show that education (X2), total annual income (X3), gender (X6), participation in various organizations like the club, societies, etc. (X8), health consciousness of individual (X11), perception of organic food (X13); could explain the major share of ~62.1% of the variation in dependent variable i.e. knowledge level.ConclusionThe developed standardized knowledge test for the present study was found valid and appropriate research tool for evaluating the knowledge level of urban citizens regarding organic food. The majority of respondents had a positive attitude towards organic food but possessed low to medium knowledge levels regarding organic food. Occasional awareness campaigns and capacity-building programs regarding various aspects of organic food in educational institutes, residential societies and through mass media can be beneficial to society.
Epigenetic MMR defect identifies a risk group not accounted for through traditional risk stratification algorithms in endometrial cancer
Authors: Riedinger Courtney J. , Brown Morgan , Haight Paulina J. , Backes Floor J. , Cohn David E. , Goodfellow Paul J. , Cosgrove Casey M. 
Published: April 2023

PurposeWe sought to evaluate the contribution of mismatch repair (MMR) status to traditional risk stratification algorithms used to predict nodal involvement and recurrence in a large single-institution cohort.MethodsEndometrioid endometrial cancer (EC) cases from 2014-2020 were evaluated. MMR immunohistochemistry (IHC) was performed universally. Uterine factors assessed in the Mayo criteria were used to retrospectively classify patients as low or high risk for lymphatic spread. Patients were classified according to risk for recurrence using GOG 99 and PORTEC criteria. Associations were evaluated using chi-square and t-tests and contributing factors assessed using logistic regression models.Results1,514 endometrioid EC were evaluated; 392 (25.9%) were MMR (MMR) deficient of which 80.4% of MMR defects were associated with epigenetic silencing of MLH1. Epigenetic MMR defects were significantly more likely to be high risk for lymph node (LN) metastasis based on Mayo criteria (74.9% vs 60.6%, p=&lt;0.001) and with the presence of LN metastasis (20.3 vs 10.5%, p=0.003) compared to MMR proficient tumors. Tumors with epigenetic MMR defects were significantly more likely to be classified as high or high intermediate risk using GOG99 and PORTEC criteria. Furthermore, cases with epigenetic MMR defects classified as low or low intermediate risk were significantly more likely to recur (GOG99 p=0.013; PORTEC p=0.008) and independently associated with worse disease-free survival (DFS). MMR status was found to be independently associated with worse DFS (HR 1.90; 95% CI 1.34-2.70; p=0.003) but not overall survival.ConclusionWhile MMR deficient EC has been associated with poor prognostic features in prior reports; we demonstrate that only epigenetic MMR defects have poorer outcomes. Epigenetic MMR defect were independently associated with lymph node metastasis after controlling for risk criteria. Epigenetic MMR deficiency was found to be an independent predictor of recurrence beyond the factors considered in traditional risk stratification algorithms. Traditional uterine-based risk stratification algorithms may not fully reflect the risk for recurrence in MMR deficient tumors. Consideration should be given to implementing MMR status and MLH1 hypermethylation alongside traditional risk stratification algorithms. Performing MMR IHC on preoperative pathologic specimens may aid in risk stratification and patient counseling.
A novel image integration technology mapping system significantly reduces radiation exposure during ablation for a wide spectrum of tachyarrhythmias in children
Authors: He Jiang , Yi Zhang , Meiting Li , Huiming Zhou , Jinhao Li , Danlei Chen , Xiaomei Li 
Published: April 2023

ObjectiveRadiofrequency catheter ablation (RFCA) has evolved into an effective and safe technique for the treatment of tachyarrhythmia in children. Concerns about children and involved medical staff being exposed to radiation during the procedure should not be ignored. “Fluoroscopy integrated 3D mapping”, a new 3D non-fluoroscopic navigation system software (CARTO Univu Module) could reduce fluoroscopy during the procedure. However, there are few studies about the use of this new technology on children. In the present study, we analyzed the impact of the CARTO Univu on procedural safety and fluoroscopy in a wide spectrum of tachyarrhythmias as compared with CARTO3 alone.MethodsThe data of children with tachyarrhythmias who underwent RFCA from June 2018 to December 2021 were collected. The CARTO Univu was used for mapping and ablation in 200 cases (C3U group) [boys/girls (105/95), mean age (6.8 ± 3.7 years), mean body weight (29.4 ± 7.9 kg)], and the CARTO3 was used in 200 cases as the control group (C3 group) [male/female (103/97), mean age (7.2 ± 3.9 years), mean body weight (32.3 ± 19.0 kg)]. The arrhythmias were atrioventricular reentrant tachycardia (AVRT, n = 78), atrioventricular node reentrant tachycardia (AVNRT, n = 35), typical atrial flutter (AFL, n = 12), atrial tachycardia (AT, n = 20) and ventricular arrhythmias [VAs, premature ventricular complexes or ventricular tachycardia, n = 55].Results① There was no significant difference in the acute success rate, recurrence rate, and complication rate between the C3 and C3U groups [(94.5% vs. 95.0%); (6.3% vs. 5.3%); and (2.0% vs. 1.5%); P &gt; 0.05]. ② The CARTO Univu reduced radiation exposure: fluoroscopy time: AVRT C3: 8.5 ± 7.2 min vs. C3U: 4.5 ± 2.9 min, P &lt; 0.05; AVNRT C3: 10.7 ± 3.2 min vs. C3U: 4.3 ± 2.6 min, P &lt; 0.05; AT C3: 15.7 ± 8.2 min vs. C3U: 4.5 ± 1.7 min, P &lt; 0.05; AFL C3: 8.7 ± 3.2 min vs. C3U: 3.7 ± 2.7 min, P &lt; 0.05; VAs C3: 7.7 ± 4.2 min vs. C3U: 3.9 ± 2.3 min, P &lt; 0.05. Corresponding to the fluoroscopy time, the fluoroscopy dose was also reduced significantly. ③ In the C3U group, the fluoroscopy during VAs ablation was lower than that of other arrhythmias (P &lt; 0.05).ConclusionThe usage of the “novel image integration technology” CARTO Univu might be safe and effective in RFCA for a wide spectrum of tachyarrhythmias in children, which could significantly reduce fluoroscopy and has a more prominent advantage for VAs ablation.