bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling the immunosuppressive role of splenectomy-induced miRNA hsa-7b-5p in promoting pancreatic cancer growth and metastasis
Authors: Wu Liangliang , Xie Yongjie , Ni Bo , Jin Peng , Li Bin , Cai Mingzhi , Wang Baogui , Liang Yuexiang , Wang Xiaona 
Published: May 2023

Abstract
The spleen is a vital organ in the immune system, but due to the anatomical location of the pancreas, splenectomy is often performed in conjunction with distal pancreatectomy. However, controversy remains regarding the increased risk of recurrence and metastasis after surgery. Previous studies have shown that splenectomy promotes the growth and spread of pancreatic cancer in mice by reducing the ratio of CD4 to Foxp3 and CD8 to Foxp3. Nevertheless, it remains unclear whether spleen deficiency affects other immune cells. Clinical observations have shown that splenectomy leads to an immunosuppressive status and increases the risk of recurrence and metastasis in some pancreatic cancer patients. Here, we established an orthotopic pancreatic cancer model with splenectomy and found that the tumor burden increased significantly. Flow cytometry analysis demonstrated that the percentage of MDSCs and CD8 + PD1 + T cells in tumors and peripheral blood significantly increased, while the infiltration of CD4 + T cells, CD8 + T cells, and natural killer cells decreased. Bulk sequencing analysis revealed that the expression of miRNA hsa-7b-5p increased in tumor tissues after splenectomy, which was associated with pancreatic cancer staging and immunosuppressive status. Similar results were obtained in vivo by constructing a KPC-miRNA hsa-7b-5p-sh cell line. These findings suggest that splenectomy enhances the expression of miRNA hsa-7b-5p, inhibits the tumor immune microenvironment, and promotes pancreatic cancer growth.
Isolation and characterization of the lytic Aeromonas phage HJ05,a new member of Caudovirales
Authors: Huang Jinxiu , Xu Ning , Liu Linyi , Lu Liqun 
Published: May 2023

Abstract
Phages are proposed for treating and preventing bacteria diseases largely due to their narrow spectrum of activity and capacity to multiply at the site of infection. Aeromonas hydrophila, a gram-negative and facultatively anaerobic bacterium, is widely distributed in aquatic environments causing diseases opportunistically in almost all freshwater fish displaying various symptoms including Aeromonas septicemia and ulcerative infections. In the present study, a virulent phage of A. hydrophila, designated as HJ05, from natural water was isolated and characterized. The morphological structure of HJ05 was observed by transmission electron microscopy, which indicated that the head of phage HJ05 was icosahedral with a diameter of about 77 nm, and the length of the straight and shrinkable tail could reach 193 nm. Analysis of the biological characteristics of HJ05 revealed the optimal multiplicity of infection (MOI) of 0.1, the latent period of phage was 10 min, the burst period was 40 min, and the burst size was 120 PFU/cell. The titer decreases rapidly from 45–50 ℃ indicating HJ05’s sensitivity to temperature, and optimum pH range for HJ05 replication was widely distributed from pH 5 to 11. Genome analysis of HJ05 revealed a linear doubled-stranded DNA genome of 79124 bp with a G + C content of 58.63%. Comparative genomic analysis revealed that the HJ05 genome has little similarity to any of the currently sequenced Aeromonas phage genomes, indicating that HJ05 does not belong to any of the known genera. Among 94 putative open reading frames identified in the genome, only 45 ORFs encoded proteins displaying significant homology to the known functional proteins in NCBI database. Phylogenetic relatedness analysis of two ORFs (ORF30 encoding head morphogenetic protein and ORF74 encoding DNA polymerase) did indicate HJ05 formed a sister clade with Rhizobium phages. According to the classification criteria of the International Committee on Taxonomy of Viruses, HJ05 was identified to order Caudovirales. This study suggested that HJ05 might represent a novel member of the family Caudovirales, and the lytic activity of HJ05 against A. hydrophila predicted its potential role in phage therapy toward the control of bacterial infections in aquaculture.
Suprahepatic Inferior Vena Cava Recanalization: A Case Report
Authors: Shah Priyansh , Williams Dakota , Morales Ivan , Keasler Eric , Varma Rakesh 
Published: May 2023

Abstract
Background:
Sharp recanalization for crossing short segment suprahepatic inferior vena cava (sIVC) occlusion using an endovascular route has been previously described and can be challenging. However, there are no modifications to the technique described in the literature, in case of unsuccessful attempt at endovascular recanalization.
Case Presentation:
Here, we describe the use of percutaneous direct transhepatic access into the sIVC under ultrasound guidance with eventual sharp recanalization of the occluded segment of the sIVC, in a woman with Budd-Chiari syndrome with chronic blood loss anemia.
Conclusions:
This novel approach has not been described in the literature and can serve as an important addition to guide complex sIVC recanalization.
Brain mimetic co-culture experiments identify mGluR1 dependence as a vulnerability of lung cancer brain metastasis
Authors: Hirata Eishu , Ishibashi Kojiro , Ichinose Toshiya , Kadokawa Riki , Mizutani Ryo , Iwabuchi Sadahiro , Togi Sumihito , Ura Hiroki , Shinjo Keiko , Nakayama Jun , Nanjo Shigeki , Niida Yo , Kondo Yutaka , Hashimoto Shinichi , Sahai Erik , Yano Seiji , Nakada Mitsutoshi 
Published: May 2023

Abstract
Interactions between cancer cells and glial cells play a crucial role in the formation of brain metastases; however, there are few methods to stably analyse these events in vitro over a long period of time. Here, we develop a simple and stable culture method, termed mixed-glial culture on/in soft substrate (MGS), which favourably mimics the brain microenvironment for various types of cancer. Utilizing this method, we discover that lung cancer cells become overly dependent on metabotropic glutamate receptor 1 (mGluR1) signalling in the brain microenvironment. Mechanistically, interactions with reactive astrocytes induce mGluR1 in cancer cells through the Wnt-5a / prickle planar cell polarity protein 1 (PRICKLE1) / RE1-silencing transcription factor (REST) axis. Induced mGluR1 interacts directly with epidermal growth factor receptor (EGFR), which activates extracellular signal-regulated kinase (ERK) in a glutamate-dependent manner. Notably, mGluR1 may also be an alternative target for osimertinib-resistant brain metastatic lung cancer. Our results demonstrate that MGS is a useful platform for investigating cancer-glia interactions, and that increased dependence on mGluR1 signalling may be one of the adaptation strategies, but also a vulnerability of brain metastatic lung cancer cells.
A novel likely pathogenic CLCN5 variant in Dent’s disease
Authors: Hayward Samantha , Norton Jessica , Bownass Lucy , Platt Caroline , Consortium Genomics England Research , Campbell Helen , Watson Elizabeth , Forrester Natalie , Smithson Sarah , Menon Anjali 
Published: May 2023

Abstract
Background
The majority of cases of Dent’s disease are caused by pathogenic variants in the CLCN5 gene, which encodes a voltage-gated chloride ion channel (ClC-5), resulting in proximal tubular dysfunction. We present three members of the same family and one unrelated paediatric patient with the same insertion-deletion CLCN5 variant. The identification of these patients and positive familial segregation led to the re-classification of this variant from one of unknown significance to one of likely pathogenicity.
Case presentation
A 41 year old male presented with end stage kidney failure, proteinuria and haematuria. Whole genome sequencing identified an insertion-deletion variant in CLCN5, resulting in a missense change (c.1744_1745delinsAA p.(Ala582Lys)). His brother and nephew, who both exhibited renal impairment, haematuria, proteinuria, glycosuria and nephrocalcinosis, were found to have the same variant. In addition, genetic testing of an un-related paediatric patient who presented with proteinuria and hypercalciuria, demonstrated the same variant.
Conclusions
The identification of this novel variant in four individuals with features of Dent’s disease, has led to the re-classification of the variant to one of likely pathogenicity.
Investigation of Pitting in Large-grain Niobium Samples of Different Purity
Authors: Ciovati Gianluigi , Balachandran Shreyas , Ens D , Su Yi-Feng , Dhakal Pashupati , Chetri Santosh , Lee Peter J 
Published: May 2023

Abstract
Niobium samples cut from large-grain discs with residual resistivity ratio ranging between 60 and 300 were mechanically polished and subjected to chemical treatments similar to those applied during the fabrication of superconducting radio-frequency cavities for particle accelerators. Pitting occurred during the chemical treatments and the pit density was higher in the samples of lower purity. Electropolishing resulted in the lowest density of pits, regardless of sample purity, compared to buffered chemical polishing. A correlation between high pit density and grains with 100 orientation of the exposed surface was found, however, no such correlation emerged with respect to the dislocation density.
mTOR interacts with AIF to positively regulate autophagy
Authors: Hou Bolin , Gao Quan , Huang Haiwen , Xi Zhijun , Jiang Xuejun , yang Huaiyi 
Published: May 2023

Abstract
The mechanism of the positive regulation of autophagy by mammalian target of rapamycin (mTOR) remains largely unknown. In the present study, we observed that inhibition of mTOR either genetically or pharmacologically suppressed basal and H2O2-induced autophagic processes concomitant with marked upregulation of apoptosis inducing factor (AIF) expression. In cells with mTOR knockdown, deprivation of AIF partially rescued both basal and induced autophagy. Importantly, we found that AIF interacted with either mTOR or Beclin1 and that AIF loss markedly enhanced the association of Beclin1 with VPS34, which is essential for autophagy initiation. In contrast, mTOR loss increased the binding of AIF to Beclin1, concomitantly decreasing the interaction between Beclin1 and VPS34. Collectively, the data presented here revealed a previously unnoticed autophagic regulatory pathway in which mTOR functioned as a positive regulator by directly interacting with AIF and autophagic proteins.
Predicting the Prognosis of Diffuse Axonal Injury Using Automated Pupillometry
Authors: Murase Makoto , Yasuda Shinichi , Sawano Makoto 
Published: May 2023

Abstract
Background Although previous studies have reported various predictive indicators of diffuse axonal injury (DAI), a consensus regarding the gold-standard predictive indicator has not yet been reached. The usefulness of automated pupillometry in patients with consciousness disorders has been widely reported; however, there are few reports of its use in patients with DAI. We thus investigated the usefulness of pupillary findings for predicting prognosis in DAI.Methods We included patients with a diagnosis of DAI admitted to our center from 1 June 2021 to 30 June 2022. Pupillary findings in both eyes were quantitatively measured by automated pupillometry every 2 hours after admission. We then examined the correlations between automated pupillometry values, patient characteristics, and outcomes such as the Glasgow Outcome Scale Extended (GOSE) score 6 months after injury and the time to follow commands.Results Of the 22 included patients, 5 presented oculomotor nerve palsy. Oculomotor nerve palsy was correlated with all outcomes. In contrast, Marshall computed tomography classification, injury severity score, and DAI grade were correlated with few outcomes. Some automated pupillometry values were significantly correlated with GOSE at 6 months after injury, and many values from the first 24 hours of measurement were correlated with the time to follow commands. In general, these results were not affected by adjusting for the sedation period, injury severity score, or Marshall computed tomography classification. A subgroup analysis of patients without oculomotor nerve palsy revealed that many automated pupillometry values from the first 24 hours of measurement were significantly correlated with most outcomes. The cutoff values that differentiated good prognosis (GOSE 5–8) from poor prognosis (GOSE 1–4) were a constriction velocity of 1.43 (area under the curve [AUC] = 0.81 [0.62–1], p = 0.037) and a maximum constriction velocity of 2.345 (AUC = 0.78 [0.58–0.98], p = 0.04). The cutoff values that differentiated the time to follow commands into within 7 days and 8 days or over were a percentage of constriction of 8 (AUC = 0.89 [0.68–1], p = 0.011), a constriction velocity of 0.63 (AUC = 0.92 [0.78–1], p = 0.013), a maximum constriction velocity of 0.855 (AUC = 0.9 [0.74–1], p = 0.017), and an average dilation velocity of 0.175 (AUC = 0.95 [0.86–1], p = 0.018).Conclusions Pupillary findings in DAI were a strong predictive indicator of prognosis. Quantitative measurements using automated pupillometry may facilitate the prediction of DAI prognosis.
Information transfer during mobbing: call rate is more important than the number of callers in a southern temperate passerine
Authors: Fernández Gustavo , Dutour Mylène , Carro Mariana E. 
Published: May 2023

Abstract
Many bird species vary their alarm calls according to the level of risk they face and this information can be used by receivers to respond to the mobbing event. Recent studies have also found that the number of callers can play an important role in the decision of subsequent individuals to join a mobbing event. We conducted a playback experiment to assess if differences in calling rate and number of callers elicit different behavioural responses in the house wren (Troglodytes aedon chilensis). We broadcast the alarm call of either one or two individuals, at different calling rates along the breeding season. We found that house wrens responded more frequently during playback of alarm calls at higher rates than during playback of calls at lower rates. However, focal individuals moved closer to the speaker when calls were played back at lower rates. There were no differences in the response of house wrens when we broadcast the calls of one or two individuals. These results suggest that call rates are an important element in the communication of danger in the house wren, rather than caller number.
COVID-associated complications after reconstructive breast surgery – a retrospective cohort study
Authors: Bubberman J.M. , Claessen J. , Feijen M.M.W. , Meesters-Caberg M.A.J. , Kuijk S.M.J. Van , Hulst R.R.W.J. Van der , Tuinder Stefania 
Published: May 2023

Abstract
Purpose. The COVID pandemic significantly influenced reconstructive breast surgery regimens. Many surgeries were cancelled or postponed. COVID entails not only respiratory, but also coagulative symptoms. It therefore potentially increases the risk of postoperative complications. The incidence of perioperative COVID infection and its influence on postoperative recovery after reconstructive breast surgery is still unknown.Methods. This dual center retrospective cohort study included patients that underwent reconstructive breast surgery between March 2020 and July 2021. Post-mastectomy autologous or implant-based breast reconstruction (ABR; IBR), as well as post-lumpectomy oncoplastic partial breast reconstruction (PBR) were eligible. Patient data was extracted from electronic medical records. Data regarding COVID-19 infection was collected through a questionnaire. The primary outcome was complication rate.Results. The ABR, IBR and PBR groups consisted of 113 (12 COVID-positive), 41 (2 COVID-positive) and 113 (10 COVID-positive) patients. In the ABR and PBR groups, postoperative complications occurred significantly more often in patients with perioperative COVID-infection. Especially impaired wound healing occurred significantly more often in the ABR and PBR breasts, but also at the donor site of ABR patients with perioperative COVID.Conclusion. Perioperative COVID-infection increases susceptibility to complicated wound healing after reconstructive breast surgery. A possible explanation lies in the dysregulation of haemostasis by the virus, and its direct effects on microvasculature. A hypercoagulable state results. We recommend to postpone elective breast surgery for 4–6 weeks after COVID-19 infection. Also, precautionary measures remain important to minimize the risk of perioperative COVID-19 infection.
Neoadjuvant carboplatin in triple-negative breast cancer: results from NACATRINE, a randomized phase II clinical trial
Authors: Souza Cristiano de Pádua , Carneiro Ana Suellen Barroso , Lessa Ana Cecília de Oliveira , Lacerda Domício Carvalho , Paiva Carlos Eduardo , Zorzetto Marina Moreira Costa , de Freitas Ana Julia Aguiar , Santana Iara Viana Vidigal , de Oliveira Marco Antonio , Palmero Edenir Inêz , Marques Márcia Maria Chiquitelli , Reinert Tomás 
Published: May 2023

Abstract
Background Neoadjuvant chemotherapy (NACT) is the mainstay of treatment of stages II and III triple-negative breast cancer (TNBC). There is interest in the addition of carboplatin to NACT, especially in TNBC patients with germline BRCA mutations or other genomic alterations associated with DNA repair deficiency. This study aims to evaluate if the addition of carboplatin to NACT is associated with an increase in the pathological complete response (pCR) rates in TNBC.
Methods We conducted an open-label phase II randomized clinical trial in a single center in Brazil. Patients with stage II and III TNBC were randomized in a 1:1 ratio to receive standard NACT with or without carboplatin. All the patients received doxorubicin (60 mg/m2) plus cyclophosphamide (600 mg/m2) both intravenously (i.v.) q21 days for four cycles. Patients were then randomized for additional treatment with weekly (wk) paclitaxel (80 mg/m2 i.v., for 12 cycles) plus wk carboplatin AUC 1.5 (experimental arm) or without wk carboplatin (control arm). Randomization was stratified according to gBRCA status (deleterious mutation vs. no deleterious mutations), age (< 50 vs.≥ 50 years), or AJCC 8th edition clinical stage (II vs. III). The primary endpoint was the pathologic complete response (pCR) rate. Secondary endpoints included recurrence-free survival, overall survival, and toxicity.
Results Between 2017 and 2021, 146 patients were randomized; 73 on each arm. The median age was 45 years, and 69.8% were < 50 years. Most patients (66.4%) had locally advanced stage III disease, 67.1% had T3/T4 tumors, and 56.2% had clinically positive axillary lymph nodes. Germline BRCA status was available for all patients, and 19.9% had pathogenic BRCA1/2 variants. The pCR rate (ypT0ypN0) was numerically increased by 13.7%, being 43.8% (31 of 73 patients) in the experimental and 30.1% (22 of 73 patients) in the control arm, not meeting the prespecified goal of increasing the pCR in 15% (p value = 0.08). Survival outcomes are immature. The toxicity profile was consistent with routine clinical practice. There were more dose interruptions and hematological toxicity in the carboplatin arm. However, the incidence of serious adverse effects was low, and there was no difference in treatment discontinuation between the two arms.
Conclusion The addition of carboplatin to standard NACT in stages II and III TNBC was associated with a non-statistically significant numerical increase in the pCR rate of 13.7%, consistent with other similar clinical trials. Follow-up for survival outcomes and translational research initiatives are ongoing.
A novel approach for enhancing the color and antimicrobial properties of pine and beech wood using Se-NPs
Authors: Elmaaty Tarek Abou , Swidan Abeer , Sayed-Ahmed Khaled , Zaghloul Nancy 
Published: May 2023

Abstract
Pine wood (PW) and beech wood (BW) are the most used wood in furniture and other applications owing to their unique characteristics and low machining cost. However, their biodegradability and varied moisture content limit their wider use and durability. Therefore, in this study, nanotechnology was used as a novel eco-friendly approach to enhance the durability, antimicrobial properties, and color of wood. Selenium nanoparticles (Se-NPs) were prepared in spherical shape at varied concentrations (25 and 50 mM) using an eco-friendly method in the range of 35–80 and 40–155 nm, respectively. Se-NPs formation at the nanoscale was confirmed using UV/Vis analysis, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The prepared Se-NPs were then impregnated into PW and BW for different periods ranging from 2 hrs to 1 week. The treated wood were then leached in distilled water for 14 days to eliminate excess Se-NPs from the wood surface. The treated wood surfaces were examined using energy-dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM). Se-NPs impacts on the color properties and antimicrobial activities of the treated wood were evaluated. PW treated with Se-NPs showed better antimicrobial and color characteristics than treated BW. PW samples immersed in 50 mM Se-NPs for 2 hrs showed the highest K/S values, whereas the highest antimicrobial values were obtained for those immersed at the same concentration for 2 days, and 1 week.
Isosteviol plays a protective role on hepatic ischemia and reperfusion injury in mice through MAPK/NF-κB signaling pathway
Authors: Chen Yuwei , Li Ronghua , Xu Hongjiao , guo long 
Published: May 2023

Abstract
Aims
The purpose of this paper is to study the effect of isosteviol (ISV) on mice with hepatic ischemia and reperfusion(I/R) injury and further to investigate its underlying mechanism.
Methods
The blood vessels supplying the left/middle lobe of the liver in mice were clamped to cause liver ischemia for 1h, and then conduct reperfusion for 6 h. ISV or saline was injected intraperitoneally after reperfusion. The expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-10 in serum and tissues were evaluated by ELISA and qRT-PCR. The infiltration of neutrophils and macrophages into the liver tissue was determined by flow cytometry and myeloperoxidase. Liver HE staining, TUNEL and Annexin V probe were used to determine liver injury and hepatocyte apoptosis. WB was used to investigate the activation of nuclear factor (NF)-κB and c-JunNH2 terminal kinase (JNK), p38 and extracellular regulated protein kinase (ERK), while the expression of apoptosis-related proteins (BCL-2, Bax, caspase3, cleaved-caspase3) was detected.
Results
ISV reduced AST and ALT levels to alleviate liver injury. ISV significantly reduced the release of inflammatory cytokines and the accumulation of liver neutrophils and microphages. Meanwhile, ISV can promote the expression of anti-apoptosis related protein BCL-2 and inhibit the expression of pro-apoptotic protein Bax and the activation of the protease caspase-3, and reduce the occurrence of hepatocyte apoptosis. Finally, ISV can reduce the phosphorylation level and activation of NF-κB, JNK, p38 and ERK.
Conclision
ISV inhibit the occurrence of inflammation and hepatocyte apoptosis through MAPK/NF-κB signaling pathway to relieve liver injury.