bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic architecture of reproductive traits in Russian Landrace pigs
Authors: Belous A. A. , Volkova V. V. , Reshetnikova A. A. , Otradnov P. I. , Zinovyeva N. A. 
Published: July 2023

Relevance. Currently, the development of molecular and population genetics is a relevant task. The need to identify reliable candidate genes is associated with increase in pig population and quality improvement of both breeding and meat products. In this regard, the whole-genome analysis method solves issues of the genetic determination in quantitative and economically important traits.Methods. In this study, the GWAS method was applied to reproductive traits of Landrace sows.Results. 35 reliable candidate genes with biological functionality were identified and described, located near or within the identified significant SNP, responsible for various reproductive traits of sow organisms. Genes were classified into two clusters — 20 genes belonged to Cluster 1, which was responsible for mitochondrial and coupled electron transport, ATP synthesis, as well as binding of fatty acids and tryptophan (AFF4, IL13, IL4, IRF1, SHROOM1, IL-5, UQCRQ, MRPL13, TTR, ENPEP, NOL4, PCDH7, DSG3, RASSF6, ALB, AFP, ANKRD17, SOX9), and 15 genes belonged to Cluster 2, which was associated with response to bacterial and viral infections (YTHDC2, KIF3A, EYA1, DSG2, DSG4, PPIH, RNF125, TRAPPC8, PITX2, KIAA1462, MTPAP, JMJD6, METTL23, SRSF2, and U2AF1).
Physical and chemical indicators and technological properties of cowʼs milk depending on the linear origin
Authors: Gorelik O. V. , Gorelik A. S. , Fedoseeva N. A. , Temerbaeva M. V. 
Published: July 2023

Relevance. Milk is not only one of the most consumed food sources for Russian citizens, but also a valuable raw material for food production. Its composition is influenced by many factors, including origin. To obtain it, dairy cattle are used, including Holstein, created by absorbing the breeding stock of domestic black-and-white cattle by bulls of the Holstein breed. The study of physicochemical parameters and technological properties of milk of cows of a new genetic formation of dairy cattle in the Middle Urals zone is of both scientific and practical interest.Results. As a result of the research, it was found that the best indicators for the nutritional value of milk were found in the milk of the cows of the Montvik Chieftain line, the calorie content of which was higher than that of the milk of cows of other lines by 1.7 and 1.3%. It had more MJ, MDB, which had a positive effect on the content of dry matter and SOMO in the milk of cows of this line. Significant differences were found in the content of fat (MJ) and protein (MDP) in the milk of cows, as well as casein in favor of milk obtained from cows of the Montvik Chieftain line (р ≤ 0.05–0.01, respectively, in terms of indicators). The increased content of protein in the milk of cows of this group allows us to speak about the effectiveness of its use in cheesemaking, which is confirmed by indicators of cheese suitability and rennet coagulability. In the milk of cows of the Montvik Chieftain line, the duration of rennet clotting of milk (the duration of clot formation) was shorter by 7–8 min. according to Dilanyan and by 7'43''–10'11'' — according to N.V. Barabanshchikov).
Comparative genetic characteristics of microsatellite profile of holstein and purebred kholmogorsky bulls
Authors: Nikolaev S. V. , Yaluga V. L. 
Published: July 2023

Relevance. Due to widespread metisation the Kholmogorskaya breed is on the verge of complete extinction, which requires the development of measures aimed at preserving not only the animals themselves, but also their genetic diversity. Microsatellites can be considered the most convenient for analyzing the genetic state of a population, due to the high level of their polymorphism.Methods. The material for genetic examination was cryocon-served sperm of bulls-producers belonging to the RSUP «Komi» for breeding work (Syktyvkar). Samples from 64 pure-bred Kholmogorsky and 36 with different levels of Holstein breeding bulls were selected for analysis. The allelic structure of STR markers was determined in the laboratory of DNA technologies of FGBNU «VNIIplem». The genetic and statistical analysis was carried out according to the methods generally accepted in biology and animal science.Results. Among purebred bulls 9 privat-alleles were identified, and in crossbreed producers — 13. The most polymorphic among Holstein animals were loci TGLA122 (Na = 10) and TGLA227 (Na = 11), in purebred animals — TGLA227 (Na = 9) and TGLA53 (Na = 10). The maximum correspondence between the number of actual and effective alleles was observed at the BM1824 locus, and the minimum at TGLA227. The Shannon index was the highest for the locus TGLA122 (2,046) in crossbreeds and for TGLA53 (2,011) in purebred bulls, and the lowest for BM1818 (1,050 and 1,174). The diversity of the allelofund of the analyzed loci in the metalized animals was slightly higher (by 0.26). The average fixation index in crossbreeds deviated towards a slight deficit of heterozygosity (0.017), and in purebred bulls an excess (-0.025). The greatest genetic relationship between the two samples was observed at the SPS115 locus (0.996), and the smallest at CSSM66 (0.827).
The influence of biologized crop rotations on organic matter and agrophysical soil layers in the arid steppe of the Southern Urals
Authors: Kaipov Ya. Z. , Sultangazin Z. R. , Akchurin R. L. 
Published: July 2023

Relevance. The continuing deterioration of the state of agricultural lands and the natural environment in general requires a transition to conservation agriculture, of which biologized crop rotations are a part.The purpose of the research is to establish the influence of biologized crop rotations on the content of organic matter and agrophysical properties of the soil in the arid steppe of the Southern Urals.Methods. Field experiments and laboratory analyses were carried out according to generally accepted methods. The soil of the experimental field is ordinary medium loamy chernozem. The climate is arid. During the years of research, the hydrothermal coefficient was 0.27–0.65. In the experiment, we studied sevenfield biologized crop rotations with four grain fields, two alfalfa fields and pure steam (type I), with five grain fields and two alfalfa fields (type II). As a control, a six-field grain-pair crop rotation was taken. We created food backgrounds: without fertilizers, organic, mineral.Results. It was revealed that biologized crop rotations in the alfalfa link of the first and second years of use accumulate 7.8–9.1 t/ha of crop-root residues in the soil layer 0-40 cm, which is three to seven times more than the grain-pair crop rotation. The increased amount of PKO in biologized crop rotations has a loosening effect on the arable (0–30 cm) and sub-arable (30–40 cm) soil layers. The density of the arable layer is 1.10 g/cm³, the sub — arable layer is 1.05 g/cm³. Accordingly, the indicators of grain — pair crop rotation are 1.12 g/cm³ and 1.12 g/cm³, or 6% more. Biologized crop rotations contained higher moisture reserves in the soil. Spring reserves of productive moisture in the soil layer of 0–100 cm in biologized crop rotations — from 97 to 112 mm for fertilizer backgrounds, which is 8–22 mm more than in the grain-steam crop rotation. Thus, biologized crop rotations in the conditions of the arid steppe of the Southern Urals contribute to the optimization of agrophysical properties and improvement of the water regime of the soil.
The effect of crop rotations and mineral fertilizers on the fertility of chestnut soil
Authors: Morozov N. A. , Khodzhaeva N. A. , Prokhorova I. V. , Khripunov A. I. , Obshchiya E. N. 
Published: July 2023

Relevance. The task of preserving and increasing soil fertility is urgent, since it is closely related to crop yields.Methods. The research was carried out in the arid zone of the Stavropol Territory at the Prikumskaya experimental breeding station in four 6-pole crop rotations from 1969 to 2022.The purpose of the research is to study the effect of saturation of grain crop rotations with pure steam on various nutrition backgrounds on the fertility of chestnut soil in the conditions of the Eastern Caucasus.Results. Over 53 years of research, the content and reserves of humus in the arable soil layer in grain-steam crop rotations decreased by 0.18–0.22% and 4.7–5.8 t/ha at the control, on a fertilized background — 0.04–0.08% and 1.0–2.0 t/ha, and in crop rotation without pure steam, on the contrary, increased, respectively, by 0.05% and 1.3 t/ha and 0.21% and 5.5 t/ha. On a natural background, the mineralization of organic matter took place much more intensively than on the variants with the introduction of fertilizers. Nitrate nitrogen reserves in crop rotations with 50.0% and 33.3% of pure steam decreased by 7.6 kg/ha and 1.3 kg/ha at the control, and on a fertilized background in crop rotation with 50.0% of pure steam — by 3.5 kg/ha. In other variants of the experiment, the amount of nitrogen increased. The maximum content of this element on all food backgrounds was noted in the crop rotation without pure steam. In all variants of the experiment, the content of mobile phosphorus and exchangeable potassium increased at the control by 0.7–2.5 and 31–62 mg/kg, and on a fertilized background by 7.2–20.0 and 12–38 mg/kg of soil. The application of mineral fertilizers contributed to an increase in phosphorus reserves by 13.6–44.6 kg/ha and a decrease in potassium by 20–63 kg/ha compared to the control.
Some Features of Potassium Migration in the Agro-Landscape
Authors: Ivanov D. A. , Rublyuk M. V. , Karaseva O. V. 
Published: July 2023

Relevance. The problem of the influence of land use methods on the loss of potassium by soils, one of the main elements in the triad of plant nutrition, is considered.Methods. The studies were carried out in 1996–2020 on the basis of monitoring the potassium content in the soils of the Gubino VNIIMZ agro-polygon located within the limits of a finite moraine hill. The monitoring was carried out on an agro-ecological transect — an industrial array that crosses the main landscape positions of the hill and consists of 10 parallel fields, each of which has an individual history. Potassium was determined in each field at 30 sampling points located 40 m apart, which made it possible to assess the impact of natural and anthropogenic conditions on it. Correlation coefficients and regression models of the dependence of potassium losses on the totality of anthropogenic factors in various microlandscapes were calculated.Results. It is established that the nature of potassium migration depends on time, the characteristics of the natural conditions of the field and the history of its use. In the mode of extensive cultivation of crops, its content in soils is significantly reduced. Within the agricultural landscape, the spatial dynamics of losses is influenced by the diversity of natural conditions, while the nature of the history of fields affects migration flows only in certain parts of it. The features of the agricultural landscape that intensify potassium losses are a light granulometric composition and the dominance of eluvial processes. In transit and accumulative geocomplexes, potassium losses are lower. Based on the regularities obtained, it is possible to develop measures for the adaptive placement of land and crops, which makes it possible to minimize the loss of potassium.
Changes in yield and its structure elements of winter bread wheat depending on the conditions of a moisture supply and a genotype
Authors: Samofalov A. P. , Podgorny S. V. , Skripka O. V. , Gromova S. N. , Chernova V. L. 
Published: July 2023

Relevance. In this regard, it is of great relevance to study influence of individual arid situations on the yield and its structure elements for winter bread wheat varieties; to identify more reliable selection criteria in breeding for drought resistance; to determine differences in the drought conditions’ response among genotypes with contrasting length of a vegetation period.Methods. The study was carried out in 2017–2021 on the fields of the research crop rotation of the FSBSI «ARC “Donskoy”», according to a green manure fallow due to the methods of SVT and a field trial. The objects of the research were 25 modern varieties and promising breeding lines of our own breeding.Results. There have been considered the types of droughts and their influence on the yield and its structural elements through the years of research in comparison with the control year 2017, which was favorable in precipitation and temperature conditions in all vegetation and development periods of winter wheat with HThC = 0.92–1.46. The yield decrease amounted to 39.4% in 2019 vs. 2017 and was due to a decrease in the number of sprouted plants and productive heads on 50.5% and 37.2%. In 2020, the productivity reduced on 10.7% due to head and plant productivity decrease on 31.8% and 12.1% and a grain size on 21.0%. In 2021, the yield decreased on 18.6% due to a decrease in plant density and productive head stand on 41.7% and 18.6%, and 1000-grain weight on 15.0%. Based on the correlation analysis, there have been identified the structural elements which could make the main contribution to productivity formation depending on the type of drought, namely plant density (r = 0.78), productive head (r = 0.70), grain size of a head and a plant (r = 0.60 and r = 0.79), plant height (r = 0.70).
Evaluation of the lines of the control nursery of spring soft wheat in arid conditions North of Kazakhstan
Authors: Fedorenko E. N. , Lutchenko Zh. I. , Artys A. Yu. 
Published: July 2023

Relevance. Northern Kazakhstan is a region where the main production of spring soft wheat grain is concentrated. The creation and introduction of new varieties that meet the requirements of modern production are relevant.Methods. The objects of research are 76 breeding lines of spring soft wheat. The experience was laid in the steppe zone of the North Kazakhstan region. The climate is arid, the average annual precipitation is 240–330 mm. The soil is ordinary carbonate heavy loamy chernozem, humus — 4.5–5.0%. Records and observations were carried out according to the Methodology of the state test (2010). The mass of 1000 grains was determined according to GOST 10842-89, the gluten content — according to ST RK 1046-2008, the nature of the grain was determined on a laboratory device Wile 200.Results. The evaluation of the breeding material was carried out in harsh arid conditions according to several economically valuable characteristics: the duration of the growing season, productivity and grain quality. Six most promising lines have been identified for further study in the conditions of Northern Kazakhstan: 433 SP-2/20, 438 SP-2/20, 446 SP-2/20, 448 SP-2/20, 619 SP-2/20, 769 SP-2/20 with a yield of 24.0–27.7 kg/ha, weighing 1000 grains — 37.2–42.8 g, in kind — 771–789 g /l, gluten content — 32.32–36.32%.
Synthesis, characterization and evaluation of antioxidant, anticancer and toxicity properties of silver nanoparticles synthesized from Syzygium aromaticum
Authors: Vishal Jambulingam , Ranjani Soundharajan , Karunya Ravindranath Jenin , Hemalatha Srinivasan 
Published: July 2023

Background: Syzygium aromaticum, also known as clove, and its essential oil has already been proved to have antioxidant, anti-inflammatory and anticancer properties. Clove is used in various foods owing to its potent antimicrobial and antioxidant properties. Essential oil extracted from clove has been used in traditional medicine for treating various ailments.
Methods: In silico analyses of phytocompounds of Syzygium aromaticum namely eugenol, B-caryophyllene, gallic acid, crategolic acid, kaempferol, quercetin, cinnamaldehyde, and oleanolic acid were docked with three apoptotic proteins involved in breast cancer, namely BCL-2, BAX and APAF-1 using AUTODOCK. In addition, flower bud extract of Syzygium aromaticum was used for the synthesis of AgNPs (silver nanoparticles). The synthesized clove-silver nanoparticles were then characterized using various techniques such as Ultraviolet-visible spectrophotometry, FTIR, FESEM-EDX, DLS and zeta potential to determine the particle size, shape, crystalline structure, and stability of CL-AgNPs and tested for its anticancer potential in MCF-7 cell lines.
Results: In silico analysis predicted that phytochemicals of clove have good interactions with the apoptosis related proteins of breast cancer. In vitro assay confirmed the cytotoxic effect of the synthesized CL-AgNPs on breast cancer cells using the MCF-7 cell line with the IC50 value of 58.64 µg/ml.
Conclusion: In vitro analysis of the anticancer activity of CL-AgNPs in MCF-7 cell line supports the in silico study by proving active interactions between the phytochemicals of clove and target proteins of the breast cancer and hence Syzygium aromaticum has been proved to possess potential anticancer property. Further research is needed to consider clove-silver nanoparticles as a novel drug for treating breast cancer.
Hubungan Pengetahuan dan Sikap Remaja Mengenai Gizi Seimbang dengan Status Gizi pada Masa Pandemi Covid-19
Authors: Malini Desak Made , Setiawati Tia , Alipn Kartiawati , Fitriani Nurullia 
Published: August 2023

ABSTRACT Maintaining a balanced nutritional intake is crucial to attaining the ideal nutritional status and optimal growth and development in adolescents. COVID-19 pandemic is also a driving factor for maintaining nutritional status as it will preserve the body's immune system in combating viral infections. Knowledge and attitudes related to balanced nutrition are one of the contributing factors to encourage adolescents to maintain their nutritional status. This study aimed to determine the relationship between the level of knowledge and attitudes related to balanced nutrition on nutritional status in adolescents aged 17-20 years. The research design used was a cross-sectional approach and the data were analyzed by the Chi-square test (α = 0.05). The results showed that there was no significant relationship between the level of knowledge and attitudes related to animal protein, fiber and vitamins, micro and macro minerals, general guidelines for balanced nutrition, and the four pillars of balanced nutrition on nutritional status (α> 0.05). It can be concluded that the level of knowledge and attitudes of adolescents does not directly influence the nutritional status of adolescents aged 17-20 years during the COVID-19 pandemic. Keywords: Adolescents Attitude, Adolescents Knowledge, COVID-19, Nutritional Status ABSTRAK Menjaga asupan gizi yang seimbang merupakan hal yang krusial untuk memiliki status gizi yang ideal dan untuk mencapai pertumbuhan dan perkembangan remaja yang optimal. Kondisi pandemi COVID-19 menjadi faktor pendorong untuk tetap mempertahankan status gizi karena dapat menjaga sistem imunitas tubuh dalam melawan infeksi virus. Pengetahuan dan sikap terkait gizi menjadi salah satu faktor yang berkontribusi untuk mendorong remaja dalam menjaga status gizinya. Penelitian ini bertujuan untuk mengetahui hubungan antara tingkat pengetahuan dan sikap terkait gizi seimbang dengan status gizi pada remaja berusia 17-20 tahun. Metode penelitian yang digunakan adalah pendekatan cross-sectional dan data dianalisis dengan uji Chi-square (α = 0,05). Hasil penelitian menunjukkan tidak ada hubungan yang signifikan antara tingkat pengetahuan dan sikap terkait protein hewani, serat dan vitamin, mikro dan makro mineral, pedoman umum gizi seimbang, dan empat pilar gizi seimbang dengan status gizi (α>0,05). Hal ini dapat disimpulkan bahwa tingkat pengetahuan dan sikap remaja tidak memberikan pengaruh secara langsung dengan status gizi remaja berusia 17-20 tahun di masa pandemi COVID-19. Kata Kunci: COVID-19, Sikap Remaja, Status Gizi, Tingkat Pengetahuan Remaja
Kajian Pustaka Montelukas: Pengobatan Asma pada Anak
Authors: Devi Selinda Anggia , Herawati Fauna Herawati 
Published: August 2023

ABSTRACT Chronic airway inflammation is a major characteristic of asthma. Montelukast is a class of leukotriene receptor antagonists (LTRA) which has the most widely used anti-inflammatory properties because it shows the best effectiveness and safety. Many in vitro and in vivo experimental clinical studies have been published in the last 10 years covering the effectiveness and safety of montelukast in childhood asthma of varying degrees of severity. Montelukast is used as an alternative monotherapy for the ICS class or as an adjunct to enhance the anti-inflammatory and sparing effects of corticosteroids. montelukast as a treatment for asthma in children can be given in cases of mild to moderate persistent asthma, wheezing due to viruses, asthma due to exercise, and intolerance of steroid use in children. The advantages of montelukast as monotherapy are fast onset in controlling asthma, low side effects, given once daily oral administration without the need for an inhaler thereby increasing pediatric patient compliance. Keyword: Asthma, Children, Montelukast, Cysteinyl Leukotriene Receptor Antagonist. ABSTRAK Inflamasi saluran napas kronis merupakan karakteristik utama asma. Montelukas merupakan golongan antagonis reseptor leukotrien (LTRA) memiliki sifat antiinflamasi yang paling banyak digunakan karena menunjukkan efektivitas dan keamanan terbaik. Penelitian klinis eksperimental baik in vitro maupun in vivo sudah banyak diterbitkan dalam 10 tahun terakhir mencakup efektivitas dan keamanan montelukas pada asma anak dengan derajat keparahan berbeda-beda. Montelukas digunakan sebagai monoterapi alternatif untuk golongan ICS atau sebagai tambahan untuk meningkatkan efek antiinflamasi dan meminimalisir penggunaan kortikosteroid. Montelukas sebagai pengobatan asma pada anak dapat diberikan pada kasus asma ringan sampai dengan persisten sedang, sesak oleh karena virus, dan asma akibat aktivitas berat, serta kondisi intoleransi steroid. Keuntungan montelukas sebagai monoterapi antara lain memiliki waktu onset cepat dalam mengendalikan asma dengan efek samping rendah pada pemberian oral sehari sekali tanpa penggunaan inhaler sehingga meningkatkan kepatuhan pasien anak. Kata Kunci: Asma, Anak, Montelukas, Cysteinyl Leukotriene Receptor Antagonist.