bluuebunny/crossref_metadata_2025_split huggingface dataset:
COVID-19 Vaccine Acceptance in Nigeria: A Rapid Systematic Review and Meta-Analysis
Authors: Nnaemeka Victory Chizaram , Okafor Nnenna Audrey , Orababar Oluwatosin Qawiyy , Anikwe Ruth , Onwe Reuben Ogba , Uzochukwu Nneka Patricia , Tsiterimam Thomas Sambo , Nwokoye Nkiru Nenye , Ike Anthony Chibuogwu 
Published: February 2023

AbstractWidespread COVID-19 vaccination is essential to maintaining pandemic control. However, low- and lower-middle-income countries (LMICs) continue to face challenges to care due to unequal access and vaccine fear despite the introduction of safe and effective immunizations. This study aimed to collect information on Nigeria’s COVID-19 vaccine uptake rates and determinants. Science Direct, PubMed, Google Scholar, African Journal Online, Springer, and Hinari were all systematically searched through and completed in May 2022. Quality assessments of the listed studies were performed using the eight-item Joanna Briggs Institute Critical Appraisal tools for cross-sectional studies. In addition, we undertook a meta-analysis to calculate pooled acceptance rates with 95% confidence intervals (CI). Forty-two studies in total satisfied the inclusion criteria and were reviewed. A total of 24,533 respondents were studied. The total sample size of states in the Northern, Western and Southern parts of Nigeria are 3,206, 4,527 and 5,059, respectively, while 11,741 is the cumulative sample size of all the Nigeria-wide studies. The total COVID-19 vaccination acceptance rate among all the study groups was 52.4% (95% CI: 46.9-57.9%,I2= 100%), while the total estimated COVID-19 vaccination hesitancy rates was 47.81% (95% CI: 42.2 – 53.4%I2= 100%). In Nigeria-regions sub-group analyses, the Western region (58.90%, 95% CI: 47.12–70.27%) and Northern region (54.9%, 95% CI: 40.11%–69.4%) showed the highest rates of vaccine acceptance and vaccine hesitancy respectively. The COVID-19 vaccine acceptance rate was highest in 2020, with a pooled rate of 59.56% (46.34, 57.32%,I2= 98.7%). The acceptance rate in 2021 was only 48.48 (40.78%, 56.22%), while for the studies in 2022, it increased to 52.04% (95% CI: 35.7%, 68.15 %). The sensitization of local authorities and the dissemination of more detailed information about the COVID-19 vaccine and its safety, could significantly increase the country’s vaccination rate.
Does early childhood BCG vaccination improve survival into adulthood in a population with a low tuberculosis prevalence? Quasi-experimental evidence on non-specific effects from 39 Swedish birth cohorts
Authors: Theilmann Michaela , Geldsetzer Pascal , Bärnighausen Till , Sudharsanan Nikkil 
Published: February 2023

AbstractThe Bacillus Calmette–Guérin (BCG) vaccine for tuberculosis (TB) is widely used globally. Many high-income countries discontinued nationwide vaccination policies as the TB prevalence decreased. However, there is continued interest in whether the general childhood immunity boost conferred by the BCG vaccination impacts adult health and mortality in low-TB contexts (known as non-specific effects) and whether BCG vaccination should be continued as a population policy. While recent studies found evidence of an association between BCG vaccination and later-life survival, it is unclear whether these associations are causal or driven by unobserved characteristics of those who chose to voluntarily vaccinate. We use the abrupt discontinuation of mandatory BCG vaccination in Sweden in 1975 as a natural experiment to estimate the causal non-specific effect of the BCG vaccine on long-term cohort survival. Applying two complementary study designs, we find no evidence that survival to age 30 was affected by the discontinuation of childhood BCG vaccination. The results are consistent in the male and female subpopulations and are robust to several sensitivity and falsification tests. Overall, despite interest and prior correlational studies suggesting large non-specific effects, we do not find any population-level evidence for a non-specific effect of the BCG vaccine discontinuation on long-term survival in Sweden.
Post Stroke Motor Recovery Genome Wide Association Study:A Domain-Specific Approach
Authors: Aldridge Chad M. , Robynne Braun , Keene Keith L. , Hsu Fang-Chi , Sale Michele M. , Worrall Bradford B. 
Published: February 2023

AbstractBackgroundIn this genome wide association study (GWAS) we aimed to discover single nucleotide polymorphisms (SNPs) associated with motor recovery post-stroke.MethodsWe used the Vitamin Intervention for Stroke Prevention (VISP) dataset of 2,100 genotyped patients with non-disabling stroke. Of these, 488 patients had motor impairment at enrollment. Genotyped data underwent strict quality control and imputation. The GWAS utilized logistic regression models with generalized estimating equations (GEE) to leverage the repeated NIH Stroke Scale (NIHSS) motor score measurements spanning 6 time points over 24 months. The primary outcome was a decrease in the motor drift score of ≥ 1 vs.<1 at each timepoint. Our model estimated the odds ratio of motor improvement for each SNP after adjusting for age, sex, race, days from stroke to visit, initial motor score, VISP treatment arm, and principal components.ResultsAlthough no associations reached genome-wide significance (p<5 × 10−8), our analysis detected 115 suggestive associations (p<5 × 10−6). Notably, we found multiple SNP clusters near genes with plausible neuronal repair biology mechanisms. The CLDN23 gene had the most convincing association which affects blood-brain barrier integrity, neurodevelopment, and immune cell transmigration.ConclusionWe identified novel suggestive genetic associations with the first ever motor-specific post stroke recovery GWAS. The results seem to describe a distinct stroke recovery phenotype compared to prior genetic stroke outcome studies that use outcome measures, like the mRS. Replication and further mechanistic investigation are warranted. Additionally, this study demonstrated a proof-of-principle approach to optimize statistical efficiency with longitudinal datasets for genetic discovery.
Effectiveness of SARS-CoV-2 Vaccines against Omicron Infection and Severe Events: A Systematic Review and Meta-Analysis of Test-Negative Design Studies
Authors: Song Shangchen , Madewell Zachary J. , Liu Mingjin , Longini Ira M. , Yang Yang 
Published: February 2023

AbstractBackgroundEvaluating vaccine effectiveness (VE) of a full vaccine series and booster doses against COVID-19 is important for health decision-making.MethodsWe systematically searched papers that evaluated VE of SARS-CoV-2 vaccines on PubMed, Web of Science, Cochrane Library, Google Scholar, Embase, Scopus, and preprint servers (bioRxiv and medRxiv) published from November 26th, 2021 to June 27th, 2022 (for full doses and first booster), and to January 8th, 2023 (for the second booster). The pooled VE against Omicron-associated symptomatic or any infection as well as severe events are estimated in a meta-analysis framework.ResultsFrom 2,552 citations identified, a total of 42 were included. The vaccination of the first booster provided stronger protection against Omicron than the full doses alone, shown by the VE estimates of 53.1% (95% CI: 48.0−57.8) vs. 28.6% (95% CI: 18.5−37.4) against infection and 82.5% (95% CI: 77.8−86.2) vs. 57.3% (95% CI: 48.5−64.7) against severe disease. The second booster offered strong protection among adults within 60 days of vaccination against infection (VE=53.1%, 95% CI: 48.0−57.8) and severe disease (VE=87.3% (95% CI: 75.5-93.4), comparable to the first booster with corresponding VE estimates of 59.9% against infection and 84.8% against severe disease. The VEs of the booster doses against severe disease among adults sustained beyond 60 days, 77.6% (95% CI: 69.4−83.6) for the first and 85.9% (95% CI: 80.3−89.9) for the second booster. The VE against infection was less sustainable regardless of dose type. Pure mRNA vaccines provided comparable protection to partial mRNA vaccines, but both provided higher protection than non-mRNA vaccination.ConclusionOne or two booster doses of current SARS-CoV-2 vaccines provide considerable protection against Omicron infection and substantial and sustainable protection against Omicron-induced severe clinical outcomes.FundingUS CDC U01 CK000670
CHARACTERISTICS OF ACUTE PSYCHIATRIC DISORDERS IN PATIENTS WITH COVID-19 IN A THIRD-LEVEL HOSPITAL IN PERU
Authors: De La Cruz-Amador Marcionila Estelita , Aguirre-Quispe Wilfor , Apaza-Aceituno Edwin Genaro , Valdivia-Francia María Francesca 
Published: February 2023

ABSTRACTObjectiveTo describe the sociodemographic and clinical characteristics of acute psychiatric disorders in COVID-19 patients in an emergency department at a national reference psychiatry and mental health hospital.MethodsA descriptive observational study was performed. Data were collected from medical records of patients admitted by emergency according to the International Classification of Diseases (ICD-11). The group of patients with a first acute psychiatric episode vs. patients with more than one acute psychiatric episode were compared.Results110 patients were included; 61.8% corresponded to the female sex and the mean age was 36 ± 12.3 years. 49.1% corresponded to schizophrenia, followed by acute polymorphic psychotic disorder (13.6%), bipolar disorder (10%), and depressive episodes (7.3%). Psychotic disorders and depressive episodes occurred in a higher percentage in the group with a first episode, 42.4% (p< 0.001), and 15.2% (p< 0.001), respectively. The episodes of schizophrenia were higher in the group of patients with previous episodes (63.6%).ConclusionsA higher frequency of cases of acute psychotic disorder and depressive disorders was found as the first episode in patients with COVID-19 infection; however, within the group with previous episodes, greater predominance of patients with acute disorders due to schizophrenia was found.
Cost-effectiveness of use of 20-valent pneumococcal conjugate vaccine among adults in Germany
Authors: Kühne Felicitas , Achtert Katharina , Püschner Franziska , Urbanski-Rini Dominika , Schiller Juliane , Mahar Ernestine , Friedrich Josephine , Atwood Mark , Sprenger Ralf , Vietri Jeffrey , von Eiff Christof , Theilacker Christian 
Published: February 2023

ABSTRACTObjectivesDespite national recommendations for use of pneumococcal vaccines, rates of community-acquired pneumonia (CAP) and invasive pneumococcal disease (IPD) remain high in Germany. New pneumococcal conjugate vaccines (PCVs) with expanded coverage have the potential to reduce the pneumococcal disease burden among adults.MethodsUsing a Markov model, we evaluated the lifetime outcomes/costs comparing 20-valent PCV (PCV20) with standard of care (SC) vaccination for prevention of CAP and IPD among adults aged ≥60 years and at-risk adults aged 18-59 years in Germany. PCV20 also was compared with sequential vaccination with 15-valent PCV (PCV15) followed by (→) PPSV23 in a scenario analysis.ResultsOver the course of a lifetime (82 years), use of PCV20 vs. SC would prevent 54,333 hospitalizations, 26,368 outpatient CAP cases, 10,946 disease-related deaths yield 74,694 additional life-years (LYs), while lowering total medical costs by 363.2M €. PCV20 remained cost saving (i.e., dominant) versus SC even in numerous sensitivity analyses. In the scenario analysis, PCV20 also showed to be cost-saving compared to using PCV15→PPSV23.ConclusionsOne dose of PCV20 among adults aged ≥60 years and adults aged 18-59 years with moderate- and high-risk conditions would substantially reduce pneumococcal disease, save lives, and be cost saving compared with SC.HIGHLIGHTSPneumococcal disease causes significant morbidity and mortality among adults in GermanyNew, higher valent vaccines have the potential to reduce disease burden and associated costs in vulnerable populationsver a lifetime, 20-valent pneumococcal conjugate vaccine was found to be cost-saving compared with current standard of care for pneumococcal disease prevention among adults in Germany
WITHDRAWN: Exposure to an enriched environment improves colonic epithelial barrier integrity and attenuates mouse experimental colitis by modulating a Myc-driven gene regulatory network
Authors: Villaseñor-Toledo Tomás , Valle-Garcia David , Pop Romana T. , Osio-Becerro Víctor , Meza-Sosa Karla F , Serrano Carolina , Díaz de León-Guerrero Sol , Hernández-Pando Rogelio , Nava Porfirio , Kuijjer Marieke L. , Pérez-Martínez Leonor , Pedraza-Alva Gustavo 
Published: February 2023

Withdrawal StatementThe authors have withdrawn their manuscript owing to erroneous labeling in two figures that have recently come to light. Experiments aiming to confirm the data presented are underway. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
Inhibition ofhaao-1enhances oxidative stress response by activating hormetic redox signaling inC. elegans
Authors: Castro-Portuguez Raul , Raymond Kayla M. , Thullen Emma , Hendrickson Alana M. , Freitas Samuel , Hull Bradford , Meyers Jeremy B. , Thorns Niall , Gardea Emily A. , Dang Hope , Espejo Luis S. , Sutphin George L. 
Published: February 2023

Abstract3-hydroxyanthranilate 3,4-dioxygenase (HAAO) is an intermediate enzyme in the conversion from tryptophan (TRP) to nicotinamide adenine dinucleotide (NAD+) via the kynurenine pathway. The kynurenine pathway is the sole‘de novo’NAD+biosynthetic pathway from ingested tryptophan. Inhibition of several enzymatic steps in the kynurenine pathway increases lifespan inDrosophila melanogaster, Saccharomyces cerevisiae, andCaenorhabditis elegans. Knockout or knockdown ofhaao-1, theC. elegansgene encoding HAAO, or supplementation of its substrate metabolite 3-hydroxyanthranilic acid (3HAA), has been shown to promote healthy lifespan extension; however, the underlying mechanism remains unknown. In the present study, we report thathaao-1knockdown induces oxidative stress resistance against several reactive oxygen species (ROS) inducing agents by activating the Nrf2/SKN-1 oxidative stress response pathway. An examination of the redox state of animals with reducedhaao-1suggests that activation of the Nrf2/SKN-1 pathway is mediated by shifting the balance toward generation of ROS, generating a hormetic effect. Our results identify a novel mechanism for an endogenous metabolite (3HAA) that activates the oxidative stress response. These results provide a conceptual basis by which modulation of the kynurenine pathway can promote healthy aging and enhanced stress resistance.Graphical AbstractHighlightsKnockdown ofhaao-1promotes oxidative stress resistance.Knockdown ofhaao-1activates the Nrf2/SKN-1 oxidative stress response.The shift in redox balance inhaao-1knockout animals suggests a hormetic mechanism for oxidative stress resistance.
Spatially localized expression of glutamate decarboxylasegadBinEscherichia coliO157:H7 microcolonies in hydrogel matrix
Authors: Martin Cédric Saint , Caccia Nelly , Darsonval Maud , Grégoire Marina , Combeau Arthur , Jubelin Grégory , Dubois-Brissonnet Florence , Leroy Sabine , Briandet Romain , Desvaux Mickaël 
Published: February 2023

AbstractFunctional diversity within isogenic spatially organized bacterial populations has been shown to trigger emergent community properties such as stress tolerance. Taking advantage of confocal laser scanning microscopy combined with a transcriptional fluorescent fusion reporting at single cell scale the expression of the glutamic acid decarboxylasegadBinE. coliO157:H7, it was possible to visualize for the first-time spatial patterns of bacterial gene expression in microcolonies grown in a gelled matrix. ThegadBgene is involved inE. colitolerance to acidic conditions and its strong over-expression was observed locally on the periphery of embedded microcolonies grown in acidic hydrogels. This spatialization ofgadBexpression did not correlate with live/dead populations that appeared randomly distributed in the colonies. While the planktonic population of the pathogens was eradicated by an exposition to a pH of 2 (HCl) for 4h, mimicking a stomachal acidic stress, bacteria grown in gel-microcolonies were poorly affected by this treatment, in particular in conditions wheregadBwas spatially overexpressed. Consequences of these results for food safety are further discussed.
A cell surface code mediates tissue-intrinsic defense against aberrant cells in epithelia
Authors: Fischer Friedericke , Ernst Laurin , Frey Anna , Holstein Katrin , Prasad Deepti , Weichselberger Vanessa , Balaji Ramya , Classen Anne-Kathrin 
Published: February 2023

AbstractTissue-intrinsic error-correction mechanisms allow epithelial cells to detect aberrant neighboring cells and cause their removal from the tissue. The molecular mechanisms which grant cells the ability to compare their internal states is unknown. Here we demonstrate that comparison of cell identity, created by cell-fate-specifying transcription factors and patterning pathways, is conveyed through a specific set of cell surface molecules. We demonstrate that Drosophila imaginal discs express a range of cell surface molecules previously implicated in neuronal axon guidance processes, such as members of the Robo, Teneurin, Ephrin, Toll-like or atypical Cadherin families. Expression of these molecules is regulated by intrinsic fate-patterning pathways of the disc but also by aberrant expression of oncogenic RasV12. Importantly, mosaic clones deregulating individual cell surface molecules are sufficient to induce all hallmarks of ’interface surveillance’, a tissue-intrinsic error-correction mechanism previously shown to be induced by cells with aberrant activation of fate-patterning pathways. Specifically, cells with deregulated expression of Robo2 and Robo3 induce actomyosin enrichment, bilateral JNK signaling and apoptosis at mosaic clone interfaces in imaginal discs. Moreover, deregulation of Robo2 levels, which is normally expressed in a complex endogenous pattern, induces these interface surveillance hallmarks in a Robo2-pattern-specific manner. Taken together, our work indicates that these cell surface molecules mediate cell fate recognition in epithelial tissues and thereby contribute to the maintenance of epithelial health by initiating detection and removal of aberrant cells during development and adult tissue homeostasis.
Redox Regulation of m6A Methyltransferase METTL3 in Human β-cells Controls the Innate Immune Response in Type 1 Diabetes
Authors: De Jesus Dario F. , Zhang Zijie , Brown Natalie K. , Li Xiaolu , Gaffrey Matthew J. , Kahraman Sevim , Wei Jiangbo , Hu Jiang , Basile Giorgio , Xiao Ling , Rana Tariq M. , Mathews Clayton , Powers Alvin C. , Atkinson Mark A. , Eizirik Decio L. , Dhe-Paganon Sirano , Parent Audrey V. , Qian Wei-Jun , He Chuan , Kulkarni Rohit N. 
Published: February 2023

SUMMARYType 1 Diabetes (T1D) is characterized by autoimmune-mediated destruction of insulin-producing β-cells. Several observations have renewed interest in the innate immune system as an initiator of the disease process against β-cells. Here, we show that N6-Methyladenosine (m6A) is an adaptive β-cell safeguard mechanism that accelerates mRNA decay of the 2’-5’-oligoadenylate synthetase (OAS) genes to control the antiviral innate immune response at T1D onset. m6A writer methyltransferase 3 (METTL3) levels increase drastically in human and mouse β-cells at T1D onset but rapidly decline with disease progression. Treatment of human islets and EndoC-βH1 cells with pro-inflammatory cytokines interleukin-1 β and interferon α mimicked the METTL3 upregulation seen at T1D onset. Furthermore, m6A-sequencing revealed the m6A hypermethylation of several key innate immune mediators includingOAS1, OAS2,andOAS3in human islets and EndoC-βH1 cells challenged with cytokines. METTL3 silencing in human pseudoislets or EndoC-βH1 cells enhanced OAS levels by increasing its mRNA stability upon cytokine challenge. Consistently,in vivogene therapy, to prolong Mettl3 overexpression specifically in β-cells, delayed diabetes progression in the non-obese diabetic (NOD) mouse model of T1D by limiting the upregulation ofOaspointing to potential therapeutic relevance. Mechanistically, the accumulation of reactive oxygen species blocked METTL3 upregulation in response to cytokines, while physiological levels of nitric oxide promoted its expression in human islets. Furthermore, for the first time to our knowledge, we show that the cysteines in position C276 and C326 in the zinc finger domain of the METTL3 protein are sensitive to S-nitrosylation (SNO) and are significant for the METTL3 mediated regulation of OAS mRNA stability in human β-cells in response to cytokines. Collectively, we report that m6A regulates human and mouse β-cells to control the innate immune response during the onset of T1D and propose targeting METTL3 to prevent β-cell death in T1D.
Bipalium admarginatumde Beauchamp, 1933 (Platyhelminthes, Tricladida, Geoplanidae) in Malaysia, with molecular characterisation including the mitogenome
Authors: Michelle Soo Oi Yoon , Gastineau Romain , Verdon George , Winsor Leigh , Justine Jean-Lou 
Published: February 2023

AbstractWe present here the first observation ofBipalium admarginatumde Beauchamp, 1933 since its original description 90 years ago. Three specimens were found on Perhentian Kecil Island, off Terengganu State, Malaysia and photographed in the field, and two were collected. This report thus includes the first colour photographs published for this species, from a locality close to the type-locality, Tioman Island (which is ca. 200 km south of the locality in this study, on the east coast of Peninsula Malaysia). We describe the external morphology and colour pattern of the species, which correspond well to the original description, itself based only on two preserved specimens. We performed an in-depth molecular characterisation of the species, including its complete mitochondrial genome, the 18S sequence and elongation 1 alpha sequence. In addition, EF1a sequences were also retrieved for 5 additional geoplanid species. NotRNA-Thrcould be detected in the mitogenome ofB. admarginatum, a lack already reported in several species of geoplanids, but we found a 13 bp sequence that contains the anticodon loop and seems to be conserved among geoplanids and might thus possibly represent a non-canonical undetected tRNA. We discuss the difficulties encountered in trying to reconstruct the cluster of nuclear ribosomal genes, a problem already mentioned for other Triclads. Three phylogenies, based respectively on all mitochondrial proteins, 18S, and EF1a, were computed; the position ofB. admarginatumwithin the Bipaliinae was confirmed in each tree, as sister-group to various bipaliine species according to the sequences available for each tree.