bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Scan, the Needle, or the Knife? National Trends in Diagnosing Stage I Lung Cancer
Authors: Lazar John F. , Adnan Sakib M. , Alpert Naomi , Joshi Shivam , Abbas Abbas E. , Bhora Faiz Y. , Taioli Emanuela , Bakhos Charles T. 
Published: December 2022

Objective: Indeterminate lung nodules have been increasingly discovered since the expansion of lung cancer screening programs. The diagnostic approach for suspicious nodules varies based on institutional resources and preferences. The aim of this study is to analyze factors associated with diagnostic modalities used for early-stage non-small cell lung cancer (NSCLC). Methods: The National Cancer Database was queried for all patients with stage I NSCLC from 2004 to 2015. Four diagnostic modalities were identified, including clinical radiography alone (CRA), bronchial cytology (BC), procedural biopsy (PB), and surgical biopsy (SB). A multivariable multinomial logistic regression was used to assess associations of patient demographics, cancer characteristics, and facility characteristics with these modalities. Results: Of 250,614 patients, 4,233 (1.7%) had CRA, 5,226 (2.1%) had BC, 147,621 (59.9%) had PB, and 93,534 (37.3%) had SB. Older patients were more likely to receive CRA (adjusted odds ratio [ORadj] = 5.3) and less likely to receive SB (ORadj = 0.73). Black patients were less likely to receive SB (ORadj = 0.83) and more likely to receive BC (ORadj = 1.31). Private insurance was associated with SB (ORadj = 1.11), whereas Medicaid was associated with BC (ORadj = 1.21). Patients more than 50 miles from the facility were more likely to undergo SB (ORadj = 1.25 vs PB; ORadj = 1.30 vs CRA; ORadj = 1.38 vs BC). Patients receiving SB had shorter days from diagnosis to treatment (23.0 vs 53.5 to 64.7 for other modalities, P < 0.001). Conclusions: Diagnostic SB to confirm early-stage NSCLC was associated with younger age, greater travel distance, and shorter time to treatment in comparison with other modalities. Black race and non-private insurance were less likely to be associated with SB.
Poor reporting quality of observational studies in children with non-syndromic cleft palate makes evidence synthesis difficult
Authors: Maina Grace , Pollock Danielle , Lockwood Craig 
Published: December 2022

Objective To assess the reporting quality of observational studies included in a systematic review of the management of chronic otitis media with effusion in children with non-syndromic cleft lip and palate using the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) checklist. Methods Medline, CINAHL, Scopus and Embase, were searched for studies comparing the use of ventilation tubes to surveillance. Two reviewers screened potential eligible articles, extracted data independently and assessed reporting quality using the STROBE checklist. Results The median compliance rate with individual STROBE items was low at 25% (range:0-100%) with 11 of the 22 items not reported in any of the studies. Items reported inconsistently pertained to potential sources of bias, sample size calculations, how loss to follow-up was addressed and management of missing data. Conclusion The development of this systematic review highlights the inadequate reporting standards in this field. Differences in the way the outcomes are defined, reported, and measured leads to variability in the observed intervention effects and difficulty in interpreting the true effect size. Future researchers are encouraged to use STROBE guidelines for the design and reporting of observational studies in this field.
Efficacy of regorafenib combined with PD-1 inhibitors in elderly patients with advanced metastatic colorectal cancer
Authors: Chen Beibei , Zhao Huichen , Huang Jinxi , Lv Huifang , Xu Weifeng , Nie Caiyun , Wang Jianzheng , Zhao Jing , He Yunduan , Wang Saiqi , Chen Xiaobing 
Published: December 2022

Abstract
Objective
This is the first clinical study that wants to investigate the treatment patterns, clinical outcomes, and prognostic factors of regorafenib plus PD-1 inhibitors therapy in Chinese elderly patients with advanced colorectal cancer.

Methods
A cohort of metastatic colorectal cancer patients 60 years or older who received treatment with regorafenib combined with PD-1 inhibitors was included in our analysis. The endpoints included overall survival (OS), progression-free survival (PFS), and prognostic factors.

Results
In total, 24 patients were enrolled with the median age of 68 years, and 62.5% were female. The median OS and PFS were 15.03 months (95% CI 7.0–23.0) and 4.0 months (95% CI 1.8–6.2), respectively. The objective response rate was 8.3%, and the disease control rate was 70.8%. Patients previously treated with regorafenib had a longer median PFS than those without (6.3 versus 2.8 months). In terms of final daily doses, it showed a trend toward better PFS (median PFS was 10.0 months) in high-dose group (daily dose above 80 mg of regorafenib) compared to low-dose group (daily dose no more than 80 mg of regorafenib) (median PFS was 3.5 months).

Conclusions
This real-world evidence confirms that Chinese elderly patients with advanced colorectal cancer may benefit from the treatment of regorafenib combined with PD-1 inhibitors, similarly with this combination therapy strategies in all age patients.
Effects of an educational program using a virtual social network on nurses’ knowledge and performance of blood pressure measurement: a randomized controlled trial
Authors: Elzeky Mohamed E. H. , Shahine Noha F. M. 
Published: December 2022

Abstract
Background
Hypertension diagnosis, treatment, and follow-up depend on accurate blood pressure measurement; however, inaccurate measurement techniques are common among healthcare providers. To improve professional performance, continuous education is necessary. Distance education through virtual social network can be used as it is easy to use and accessible.

Methods
This study adopted a randomized controlled trial design and was conducted at two hospitals in Mansoura, Egypt. The subjects were selected from two hospitals using Stratified random sampling method in proportion to the total number of nurses. Seventy nurses were included in this study and were randomly divided into the intervention (n = 35) and control (n = 35) groups using block randomization. Data were gathered before and after intervention using a blood pressure measurement knowledge questionnaire and a blood pressure measurement observation checklist. The blood pressure measurement error (the difference between the BP determined by each observer and the reference BP) was calculated for the randomly selected recordings in both groups. The intervention group received 24 blood pressure measurement education sessions using WhatsApp, while the control group received only routine education using traditional lecture.

Results
No statistically significant differences in pretest knowledge scores, performance scores, and range of error were found between both groups, whereas, after intervention, knowledge scores were higher in the intervention group than those in the control group (p < 0.001). The range of error of systolic and diastolic blood pressure values significantly reduced after the intervention in the intervention group, and the posttest performance scores were higher than the pretest performance scores; however, the difference was not statistically significant.

Conclusions
The blood pressure measurement education program via WhatsApp was effective in increasing nurses’ knowledge and reducing the range of error; however, a multimodal approach may be required to improve performance scores.

Trial registration
Prospectively registered with ClinicalTrials.gov on 09/03/2021; registration number NCT04789642.
Role of mast cells in the pathogenesis of severe lung damage in COVID-19 patients
Authors: Budnevsky Andrey V. , Avdeev Sergey N. , Kosanovic Djuro , Shishkina Victoria V. , Filin Andrey A. , Esaulenko Dmitry I. , Ovsyannikov Evgeniy S. , Samoylenko Tatiana V. , Redkin Alexander N. , Suvorova Olga A. , Perveeva Inna M. 
Published: December 2022

Abstract
Background
There is still insufficient knowledge with regard to the potential involvement of mast cells (MCs) and their mediators in the pathology of coronavirus disease-2019 (COVID-19). Therefore, our study aimed to investigate the role of MCs, their activation and protease profiles in the pathogenesis of early and late lung damage in COVID-19 patients.

Methods
Formalin-fixed and paraffin embedded lung specimens from 30 patients who died from COVID-19 and 9 controls were used for histological detection of MCs and their proteases (tryptase, chymase) followed by morphometric quantification.

Results
Our results demonstrated increased numbers of MCs at early stage and further augmentation of MCs number during the late stage of alveolar damage in COVID-19 patients, as compared to the control group. Importantly, the percentage of degranulated (activated) MCs was higher during both stages of alveolar lesions in comparison to the controls. While there was no prominent alteration in the profile of tryptase-positive MCs, our data revealed a significant elevation in the number of chymase-positive MCs in the lungs of COVID-19 patients, compared to the controls.

Conclusions
MCs are characterized by dysregulated accumulation and increased activation in the lungs of patients suffering from COVID-19. However, future profound studies are needed for precise analysis of the role of these immune cells in the context of novel coronavirus disease.
Object location learning in mice requires hippocampal somatostatin interneuron activity and is facilitated by mTORC1-mediated long-term potentiation of their excitatory synapses
Authors: Honoré Eve , Lacaille Jean-Claude 
Published: December 2022

AbstractHippocampus-dependent learning and memory originate from long-term synaptic changes in hippocampal networks. The activity of CA1 somatostatin interneurons (SOM-INs) during aversive stimulation is necessary for contextual fear memory formation. In addition, mTORC1-dependent long-term potentiation (LTP) of SOM-IN excitatory input synapses from local pyramidal cells (PC-SOM synapses) contributes to the consolidation of fear motivated spatial and contextual memories. Although, it remains unknown if SOM-IN activity and LTP are necessary and sufficient for novelty motivated spatial episodic memory such as the object location memory, and if so when it is required. Here we use optogenetics to examine whether dorsal CA1 SOM-IN activity and LTP are sufficient to regulate object location memory. First, we found that silencing SOM-INs during object location learning impaired memory. Second, optogenetic induction of PC-SOM synapse LTP (TBSopto) given 30 min before object location training, resulted in facilitation of memory. However, in mice with mTORC1 pathway genetically inactivated in SOM-INs, which blocks PC-SOM synapse LTP, TBSopto failed to facilitate object location memory. Our results indicate that SOM-IN activity is necessary during object location learning and that optogenetic induction of PC-SOM synapse LTP is sufficient to facilitate consolidation of object location memory. Thus, hippocampal somatostatin interneuron activity is required for object location learning, a hippocampus-dependent form of novelty motivated spatial learning that is facilitated by plasticity at PC-SOM synapses.
Exploring potential induction of grapevine (Vitis spp.) root phenolic compounds by ring nematodes, Mesocriconema xenoplax
Authors: Wallis Christopher M. 
Published: December 2022

Abstract
Objective
Ring nematodes can decrease vineyard productivity when plated in conditions favorable for their survival. Resistant rootstocks are available to combat harm due to ring nematodes, and compounds called phenolics were hypothesized as imparting this resistance. Therefore, this study measured phenolic compound levels in four different rootstocks and attempted to find associations with ring nematode populations. Furthermore, select phenolics called stilbenoids were tested in a bioassay to observe if these compounds affect ring nematode survival. This was part of a larger effort to assess the role of phenolics in protecting grapevines from nematodes and other pathogens or pests.

Data description
This study was conducted over 2 years, 2018 and 2019, and phenolic levels were much greater in 2019 than 2018 likely due to uncontrolled differences in climatic controls. Ring nematode infected grapevines also did not have different phenolic compound levels than healthy controls. Bioassays of different stilbenoid polymers revealed no significant effects on ring nematode survival. These results suggest that analyzed root phenolic compounds were not involved in resistance or susceptibility to ring nematodes. These data should steer future researchers into analyzing other potential sources of nematode resistance.
NAA60 (HAT4): the newly discovered bi-functional Golgi member of the acetyltransferase family
Authors: Donnarumma Federica , Tucci Valeria , Ambrosino Concetta , Altucci Lucia , Carafa Vincenzo 
Published: December 2022

AbstractChromatin structural organization, gene expression and proteostasis are intricately regulated in a wide range of biological processes, both physiological and pathological. Protein acetylation, a major post-translational modification, is tightly involved in interconnected biological networks, modulating the activation of gene transcription and protein action in cells. A very large number of studies describe the pivotal role of the so-called acetylome (accounting for more than 80% of the human proteome) in orchestrating different pathways in response to stimuli and triggering severe diseases, including cancer. NAA60/NatF (N-terminal acetyltransferase F), also named HAT4 (histone acetyltransferase type B protein 4), is a newly discovered acetyltransferase in humans modifying N-termini of transmembrane proteins starting with M–K/M-A/M-V/M-M residues and is also thought to modify lysine residues of histone H4. Because of its enzymatic features and unusual cell localization on the Golgi membrane, NAA60 is an intriguing acetyltransferase that warrants biochemical and clinical investigation. Although it is still poorly studied, this review summarizes current findings concerning the structural hallmarks and biological role of this novel targetable epigenetic enzyme.
The dynamics of three-dimensional chromatin organization and phase separation in cell fate transitions and diseases
Authors: Ling Xiaoru , Liu Xinyi , Jiang Shaoshuai , Fan Lili , Ding Junjun 
Published: December 2022

AbstractCell fate transition is a fascinating process involving complex dynamics of three-dimensional (3D) chromatin organization and phase separation, which play an essential role in cell fate decision by regulating gene expression. Phase separation is increasingly being considered a driving force of chromatin folding. In this review, we have summarized the dynamic features of 3D chromatin and phase separation during physiological and pathological cell fate transitions and systematically analyzed recent evidence of phase separation facilitating the chromatin structure. In addition, we discuss current advances in understanding how phase separation contributes to physical and functional enhancer-promoter contacts. We highlight the functional roles of 3D chromatin organization and phase separation in cell fate transitions, and more explorations are required to study the regulatory relationship between 3D chromatin organization and phase separation.
Graphical Abstract
3D chromatin organization (shown by Hi-C contact map) and phase separation are highly dynamic and play functional roles during early embryonic development, cell differentiation, somatic reprogramming, cell transdifferentiation and pathogenetic process. Phase separation can regulate 3D chromatin organization directly, but whether 3D chromatin organization regulates phase separation remains unclear.
Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease
Authors: Chen Shuo , Chang Yuzhou , Li Liangping , Acosta Diana , Li Yang , Guo Qi , Wang Cankun , Turkes Emir , Morrison Cody , Julian Dominic , Hester Mark E. , Scharre Douglas W. , Santiskulvong Chintda , Song Sarah XueYing , Plummer Jasmine T. , Serrano Geidy E. , Beach Thomas G. , Duff Karen E. , Ma Qin , Fu Hongjun 
Published: December 2022

AbstractHuman middle temporal gyrus (MTG) is a vulnerable brain region in early Alzheimer’s disease (AD), but little is known about the molecular mechanisms underlying this regional vulnerability. Here we utilize the 10 × Visium platform to define the spatial transcriptomic profile in both AD and control (CT) MTG. We identify unique marker genes for cortical layers and the white matter, and layer-specific differentially expressed genes (DEGs) in human AD compared to CT. Deconvolution of the Visium spots showcases the significant difference in particular cell types among cortical layers and the white matter. Gene co-expression analyses reveal eight gene modules, four of which have significantly altered co-expression patterns in the presence of AD pathology. The co-expression patterns of hub genes and enriched pathways in the presence of AD pathology indicate an important role of cell–cell-communications among microglia, oligodendrocytes, astrocytes, and neurons, which may contribute to the cellular and regional vulnerability in early AD. Using single-molecule fluorescent in situ hybridization, we validated the cell-type-specific expression of three novel DEGs (e.g., KIF5A, PAQR6, and SLC1A3) and eleven previously reported DEGs associated with AD pathology (i.e., amyloid beta plaques and intraneuronal neurofibrillary tangles or neuropil threads) at the single cell level. Our results may contribute to the understanding of the complex architecture and neuronal and glial response to AD pathology of this vulnerable brain region.
Student perspectives on the use of universal design for learning in virtual formats in higher education
Authors: Beck Wells Melissa 
Published: December 2022

AbstractUniversal design for learning (UDL) in higher education may be a useful tool in supporting the heterogenous higher education student population, specifically in supporting student academic outcomes and retention. With more students enrolling in digital formatted education, specifically international students, a strong framework must be established to support non-geocentric students. Using survey responses of undergraduate degree-seeking students, this mixed-methods study explored the students’ perceptions and impact of UDL in virtual learning modalities (online courses, independent studies, and virtual study groups). A sample size of 57 participants used a Likert-type survey to assess the student-reported prevalence of UDL techniques in higher education in various virtual learning modalities. Students also discussed the strategies that impacted their experience as learners and the mode of study in which they felt most successful. Implications for practice and research are also presented.
Pediatric injury due to wheeled recreational devices: a single-institution retrospective study
Authors: Nichols John Charles , Sorrentino Annalise , Hayslip Margaret , King William , Jones Angela , Monroe Kathy 
Published: December 2022

Abstract
Background
Injuries are the number one cause of death in children and cause significant morbidity. Common scenarios for injury include wheeled recreational devices (WRDs) that allow children to be mobile and independent (example ATV-all terrain vehicles, dirt bikes, bicycles, skateboards, and scooters). We present a case series review of these external causes of injury. This study aims to evaluate epidemiologic trends in WRD injuries and patterns in usage of protective gear.

Results
A total of 263 patients were identified as meeting criteria for inclusion with the following causes of injuries-103 bicycle, 73 ATV, 27 dirt bike, 14 skateboard, 13 motorcycle, 7 go carts, 3 hover board, 3 roller skates, 1 dune buggy, 1 motor scooter, 1 rip stick, and 1 tractor toy. Ages of patients ranged from 2 to 18 years of age with the greatest range being noted for bicycles (2–17 years) and motorcycles (3–18 years). The mean age was higher for motorcycle and skateboard (12.9 and 11.6, respectively) and lowest for scooter and bicycle (8.3 and 9.2, respectively). The majority of [overall study (97%) and for each mode of transportation] patients were Caucasian, which is in contrast to our overall ED population, which is only 42% Caucasian. The majority of patients were male [190 (72%)]. Over half of the overall patients, 159 of the 263 (60%), were not wearing a helmet (with only 10 charts having no documentation of helmet use). In regard to ATV riders specifically, 58% were not wearing helmets at the time of injury, with an additional 5 patients who reported their helmet came off. The lowest percentage of riders reporting appropriate helmet use was skateboarders with only 21% wearing helmets, and the highest percentage was dirt bike riders with 74% reporting helmet use.

Conclusion
Common scenarios for injury include WRDs that allow children to be mobile and independent. Most of these injuries were found in Caucasian males between the ages of 9–12 with low rates of helmet use. This study adds to the literature with a description of the breadth of products children use and are injured while using.
Impact of caregiver swimming capability on perceptions of swimming pool supervision of toddlers
Authors: Johnson Molly B. , Boriack Elizabeth D. , McConnell Carlee M. , Lawson Karla A. 
Published: December 2022

Abstract
Background
Drowning is a leading cause of unintentional injury-related death for toddlers within the USA. Keeping toddlers within arm’s reach while swimming is recommended, yet many caregivers do not. Possibly, caregivers’ attitudes are shaped by their expectations about whether they could quickly save a child. The aims of this study are to 1) explore caregivers’ views of arm’s reach pool supervision in various scenarios and 2) understand whether perceptions of arm’s reach pool supervision are impacted by the caregiver’s self-reported capability to swim the length of a standard pool.

Results
Caregivers generally showed agreement with arm’s reach pool supervision; however, arm’s reach supervision was viewed as less necessary when a toddler was in shallow water, wearing a flotation device, or with an older child or teen. There was a significant effect of caregiver swimming capability on perceptions of arm’s reach pool supervision, with non-swimmers and the strongest swimmers showing more positive perceptions of arm’s reach pool supervision than caregivers reporting poor swimming capability. Female caregivers showed significantly more agreement with arm’s reach pool supervision compared with male caregivers. Grandparents and parents showed significantly more agreement with arm’s reach pool supervision than siblings.

Conclusions
Caregivers’ views about what constitutes appropriate supervision are impacted by gender, the relationship to the toddler, and the caregiver’s swimming capability. Findings suggest that a caregiver’s ability to offer close supervision or respond in an emergency may influence their attitudes about what constitutes appropriate supervision. Caregivers may view arm’s reach pool supervision as less necessary when additional layers of protection are in place.