bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monocyte pathology in human tuberculosis is due to plasma milieu changes and aberrant STAT signalling
Authors: Ahor Hubert Senanu , Schulte Rebecca , Adankwah Ernest , Harelimana Jean De Dieu , Minadzi Difery , Acheampong Isaac , Vivekanandan Monika M. , Aniagyei Wilfred , Yeboah Augustine , Arthur Joseph F. , Lamptey Millicent , Abass Mohammed K. , Kumbel Francis , Osei‐Yeboah Francis , Gawusu Amidu , Debrah Linda Batsa , Owusu Dorcas O. , Debrah Alexander , Mayatepek Ertan , Seyfarth Julia , Phillips Richard O. , Jacobsen Marc 
Published: May 2023

AbstractMonocyte‐derived macrophages contribute centrally to immune protection in Mycobacterium tuberculosis infection and changes in monocyte phenotype characterize immunopathology in tuberculosis patients. Recent studies highlighted an important role of the plasma milieu in tuberculosis immunopathology. Here, we investigated monocyte pathology in patients with acute tuberculosis and determined tuberculosis plasma milieu effects on phenotype as well as cytokine signalling of reference monocytes. Patients with tuberculosis (n = 37) and asymptomatic contacts (controls n = 35) were recruited as part of a hospital‐based study in the Ashanti region of Ghana. Multiplex flow cytometry phenotyping of monocyte immunopathology was performed and effects of individual blood plasma samples on reference monocytes prior to and during treatment were characterized. Concomitantly, cell signalling pathways were analysed to elucidate underlying mechanisms of plasma effects on monocytes. Multiplex flow cytometry visualization characterized changes in monocyte subpopulations and detected higher expression of CD40, CD64 and PD‐L1 in monocytes from tuberculosis patients as compared to controls. Aberrant expression normalized during anti‐mycobacterial treatment and also CD33 expression decreased markedly. Notably, higher CD33, CD40 and CD64 expression was induced in reference monocytes when cultured in the presence of plasma samples from tuberculosis patients as compared to controls. STAT signalling pathways were affected by the aberrant plasma milieu and higher levels of STAT3 and STAT5 phosphorylation was found in tuberculosis plasma‐treated reference monocytes. Importantly, high pSTAT3 levels were associated with high CD33 expression and pSTAT5 correlated with CD40 as well as CD64 expression. These results suggested plasma milieu effects with potential implications on monocyte phenotype and function in acute tuberculosis.
A blockchain‐based framework to optimize shipping container flows in the hinterland
Authors: Mhiri Mariem , Al‐Yafi Karim , Legros Benjamin , Jouini Oualid , Haouari Mohamed 
Published: May 2023

AbstractWe address two interrelated issues affecting the hinterland portion of the maritime container supply chain: reducing the movement of empty containers and reducing empty trips by trucks carrying these containers. In this paper, we show that empty container flow optimization can be implemented via a blockchain based on the proof‐of‐useful‐work concept where the proof of work requires the solution of an ‐hard optimization problem whose solution benefits the blockchain participants. Accordingly, we propose that anonymous miners compete to solve the container truck routing problem, which seeks to find the most efficient routes for trucks. We show that this problem is ‐hard. Miners must also solve the problem of optimally matching consignees and shippers, which will reduce transportation and storage costs for empty containers. In essence, the proposed framework turns blockchain into a massive optimization engine that directly benefits the hinterland container supply chain ecosystem.
Morphological traits for allometric scaling of the European Sea Bass Dicentrarchus labrax (Linnaeus, 1758) from Southern Portugal population
Authors: Azevedo Ana , Navarro Luiz C. , Cavalheri Thaís , Santos Hugo G. , Martins Irene , Ozório Rodrigo 
Published: May 2023

AbstractThe present study aimed to determine the allometric scaling among a selection of morphological traits in European sea bass (Dicentrarchus labrax) to estimate fish body weight. A set of morphological traits (fish body weight, length, height, and width) were directly measured in 146 fish of a recirculating aquaculture system, with body weights ranging from 17.11 to 652.21 g. In addition, a collection of digital imagery of each anesthetized fish from the side and top views were used to estimate other traits (indirect measures). Multiple regression analysis and regression coefficients were calculated using all possible combinations of biometric data (predictors) to estimate fish body weight, applying different numerical fitting models (linear, log‐linear, quadratic, exponential). The results showed that the best combination of traits for estimating fish body weight were fish body width, length and height, collected from direct measure (R2 = 0.995), for a log‐linear model fitting, which revealed more accurate determinations than the most commonly used length–weight relationship. Nevertheless, other combinations of morphological traits and fitting models were also found to be suitable in successfully predict fish body weight, with variability ranging between 92.5% and 98.5%. For indirect measures, the best predictor was a combination of traits from top view (width, eye distance and area without fins) fitted with a log‐linear function. These results comprise a relevant baseline in supporting the high potential of noninvasive methods to accurately follow the growth of European sea bass juveniles, recurring to imagery analysis of anesthetized fish. It has major potential applications in feeding consumption trials and fish growth models, as it allows for continuously following up fish growth under different experimental conditions without therein distress derived from manipulation.
Levamisole hydrochloride as an anthelmintic against Neoechinorhynchus buttnerae in juvenile Colossoma macropomum
Authors: Moraes José Lima , de Andrade‐Porto Sanny Maria , Ono Eduardo Akifumi , Claudiano Gustavo Da Silva , Affonso Elizabeth Gusmão 
Published: May 2023

AbstractAcanthocephalosis is caused by the endoparasite Neoechynorhyncus buttnerae and affects fish farmed in the Amazon. This study assessed the efficacy of therapeutic levamisole hydrochloride (LVC) baths against N. buttnerae and its effects on juvenile tambaqui blood parameters. In vitro and in vivo tests were carried out, the latter employing two experimental therapeutic LVC bath protocols. Concerning in vitro efficacy, the T75 (75 mg.L−1 LVC) and T100 (mg.L−1 LVC) treatments were 100% effective in 15 min, while the T50 (50 mg.L−1 LVC) and T25 (25 mg.L−1 LVC) treatments required parasite exposure for 45 and 60 min, respectively. During exposure, the parasites displayed reduced motility, proboscis retraction, coiling into a spiral shape, body rigidity and swelling. The LVC LC50–72h for juvenile tambaqui was 115 mg.L−1. Regarding in vivo efficacy for Protocol I (8‐h bath), the T125 resulted in 82% effectiveness, while in Protocol II (two 8‐h baths with a 24‐h interval), the T115 treatment (115 mg.L−1 LVC) achieved 95.6% effectiveness without clinical intoxication signs, despite behavioural changes. No significant changes were observed in fish blood parameters. LVC was, therefore, highly effective both in vitro and in vivo in controlling the acanthocephalan N. buttnerae without compromising tambaqui juvenile homeostasis.
The pathology of Lates calcarifer herpesviral disease—Disseminated intravascular coagulation explains mortality spikes
Authors: Gibson‐Kueh S. , Awate S. , Schrittwieser M. 
Published: May 2023

AbstractLates calcarifer herpesvirus (LCHV) causes an emerging serious disease in aquaculture. Sudden drops in feed rates and mortality spikes exceeding 40%–50% often accompany LCHV infections in juvenile L. calcarifer, soon after transfer into sea cages. Affected fish have patchy white skin and fins, corneal opacity and frequently hang in surface water column like ‘ghost’ or ‘zombie’ fish. Fish have pale gills, fluid‐filled intestines with yellowish casts, lipid depleted liver, enlarged spleen and kidney and reddened brain. Epithelial hyperplasia, apoptosis, marginated nuclear chromatin, amphophilic intranuclear inclusion bodies and the occasional multinucleated cells are observed in gills, skin, intestines, liver and kidney. These are often accompanied by lymphocytic‐monocytic infiltration and extensive necrosis in gills, skin, kidney and intestines. Martius scarlet blue stains indicate presence of fibrin in vasculature in brain, gills, intestines, kidney and liver, or disseminated intravascular coagulation (DIC). DIC has been reported in human herpesviral infections. Multifocal lifting of intestinal epithelium with proteinaceous exudate and necrosis of several adjacent villi often progress to involve entire gut sections. Atrophied livers with accentuated lobules may progress to marked loss of hepatic acini. Multifocal dilated attenuated renal tubules are often accompanied by casts and marked protein loosing renopathy. This study on LCHV demonstrates that it can cause significant pathology and mortality.
Cultural, trust, and transparency effects on the use of anchoring in mergers and acquisitions
Authors: Ferris Stephen P. , Jayaraman Narayanan , Liao Min‐Yu (Stella) 
Published: May 2023

AbstractAlthough price anchoring is a global phenomenon, we find that country cultures, trust levels, and information/legal transparency affect its use in determining target offer prices. Price anchoring is associated with cultures that deemphasize long‐term orientation, uncertainty avoidance, and personal indulgence. Acquirers from countries with low levels of trust in people or the legal system are more likely to anchor their bids. Anchoring is more frequently observed in countries where information and legal transparency is poor. We find that the use of anchoring can result in reduced long‐term performance by acquirers.
Reflecting the importance of human needs fulfilment in absolute sustainability assessments: Development of a sharing principle
Authors: Heide Mia , Hauschild Michael Z. , Ryberg Morten 
Published: May 2023

AbstractAbsolute environmental sustainability assessments (AESAs) evaluate whether the environmental impact of a product system is within its share of a safe operating space as determined by biophysical sustainability limits such as the planetary boundaries (PBs). The choice of sharing principle has significant influence on the result of an AESA, and any studies call for further research on how to share the safe operating space in an operational way that relates to the product's contribution to the welfare of the user. In this study, we develop the “Fulfilment of Human Needs” (FHN) principle as a sharing principle that operationalizes sufficientarianism (making sure everyone gets enough). The FHN principle is tested on two case studies (a food item and a textile) against four of the PBs: climate change, land‐system change, water use, and nitrogen cycling. The operationalization of the FHN principle is slightly different between the PBs; the starting point for climate change is the average consumption pattern in countries classified as “most sustainable,” while for the other three PBs the status quo impact in the most sustainable countries is used. To operationalize the FHN principle on the product level, each consumption category is downscaled according to objective sources that determine the value delivered to the users. We demonstrate that, compared to other previously applied sharing principles, the FHN principle supports a stronger relation to the importance to the users of the delivered outcome.
Effect of a resistance training programme implemented with high levels of effort on physical fitness in people with intellectual disabilities living in group homes: a randomised controlled trial
Authors: Gutiérrez‐Cruz C. , Roman‐Espinaco A. , Muñoz‐López S. , Ruiz‐Perálvarez F. J. , García‐Ramos A. 
Published: May 2023

AbstractBackgroundThe objective of this study was to evaluate the effectiveness of a 14‐week resistance training programme implemented with high levels of effort to improve physical fitness in people with intellectual disabilities (IDs) living in group homes.MethodsFifty‐two individuals with mild to moderate IDs participated in the experimental (n = 27; 15 men) or control groups (n = 25; 14 men). Participants performed 2 familiarisation sessions, 1 pretest, 42 training sessions (14 weeks × 3 sessions; only the experimental group) and 1 posttest. The testing sessions comprised the evaluation of body composition, static balance and muscle strength. The training sessions included four blocks: (1) dynamic bodyweight exercises, (2) dynamic exercises performed against external loads, (3) ballistic exercises and (4) static exercises.ResultsThe main findings revealed that all variables related to body composition and muscle strength improved more after the intervention period in the experimental group than the control group, whereas the improvements in static balance for the experimental groups were lower than for the remaining variables used as markers of physical fitness.ConclusionsThese findings highlight the importance of prescribing specific moderate‐intensity to high‐intensity resistance training programmes to improve body composition and muscle strength for people with IDs living in group homes.
Comparing the efficacy of three methods of permethrin application in pediculosis capitis: A randomized clinical trial
Authors: Ghalandari Nasibeh , Edalatkhah Tatafi Aida , Mohammadnezhad Ghader , Nasimi Maryam , Esmaily Hadi 
Published: May 2023

AbstractBackgroundPediculosis capitis, also known as head lice, is a common problem that affects individuals of different socio‐economic backgrounds. Permethrin is generally considered the first‐line treatment option for head lice.AimsThe objective of this study was to evaluate and compare the therapeutic effects of three different methods of permethrin treatment for head lice.MethodA parallel, randomized clinical trial was conducted on 157 patients with head lice. The participants underwent eye examination and dry combing by a trained professional. The subjects were randomly divided into three groups and treated with one of the three methods of permethrin application: permethrin shampoo for 10 min, permethrin shampoo for 1 h, or permethrin cream for 10 min on a weekly basis for 3 weeks.ResultsOf the 157 participants, 154 completed the study. The group treated with permethrin shampoo for 1 h had the shortest average time for eradication of lice at 1.226 ± 0.422 weeks, which was significantly lower than the other two groups. Additionally, the 1‐h permethrin shampoo group had the lowest time for scalp itching of 2.15 ± 0.632 weeks, which was significantly lower than the other two groups. Moreover, the rate of lice eradication in the first week was significantly higher in the 1‐h permethrin shampoo group.ConclusionThe results of this study suggest that the use of 1% permethrin shampoo for 1 h is more effective in eradicating head lice within the first week of treatment and in relieving scalp itching during the second week.
Clinical efficacy of a combination treatment of traditional Chinese medicine for scalp seborrheic dermatitis
Authors: Zhang Fan , Li Yuan‐hong , Ren Wei , Li Shu‐run , Chen Yan‐chao 
Published: May 2023

AbstractObjectiveTo evaluate the clinical efficacy of a combination treatment of traditional Chinese medicine (TCM) in scalp seborrheic dermatitis (SSD) of differing severity.MethodsOur study included patients with typical SSD who visited the Medical Research Center for Hair and Skin at our hospital. Symptoms were evaluated using a “16‐point scale” developed at the center. Patients who had mild SSD were treated with Pi Fu Kang Xi Ye (PFKXY), those with moderate SSD were treated with PFKXY combined with Run Zao Zhi Yang Jiao Nang (RZZYJN), and those with severe dermatitis were treated with PFKXY and RZZYJN along with garlicin enteric‐coated tablets. Patients were asked to revisit 4 weeks later to evaluate the efficacy.ResultsSymptom scores of all patients decreased by (5.48 ± 2.51) after treatment as compared with before treatment, and the results of t‐test and correlation test were significant (p < 0.01). The scores of patients with mild, moderate and severe SSD decreased by 3.14 ± 1.83, 4.90 ± 1.77, and 8.05 ± 2.21, respectively, after treatment as compared with before treatment. Among them, the changes in scores of patients with moderate dermatitis before and after treatment were significant in the t‐test and correlation test (p < 0.01).ConclusionIn this study, the combination treatment of TCM showed significant efficacy in the treatment of mild, moderate, and severe SSD, and the efficacy was stable, especially for patients with moderate SSD.
The pivotal role of Bifida Ferment Lysate on reinforcing the skin barrier function and maintaining homeostasis of skin defenses in vitro
Authors: Wang Rui , Yan Shiyu , Ma Xue , Zhao Jinfeng , Han Yuqing , Pan Yao , Zhao Hua 
Published: May 2023

AbstractBackgroundThe semiactive or inactive probiotics or their extracts used in dermatology have interesting properties to ameliorate signs of irritated skin and enhance the skin barrier. Bifidobacterium, as the most common probiotics, which has been found to be effective in reducing acne and improving the skin barrier function of atopic dermatitis. Bifida Ferment Lysate (BFL) can be obtained from Bifidobacterium by fermentation and extraction.PurposeIn this study, we investigated the effect of a topically used BFL on the skin using in vitro evaluation methods.ResultsThe results showed that upregulation of skin physical barrier gene (FLG, LOR, IVL, TGM1, and AQP3) and antimicrobial peptide gene (CAMP and hBD‐2) in HaCaT cells by BFL might be responsible for skin barrier resistance. In addition, BFL had strong antioxidant properties representing a dose‐dependent increasing of the scavenging capacity of DPPH, ABTS, hydroxyl, and superoxide radicals. BFL treatment also fundamentally inhibited the intracellular ROS and MDA production and improved the activities of antioxidant enzymes (CAT and GSH‐Px) in H2O2‐stimulated HaCaT cells. As a good immunomodulatory factor, BFL efficiently decreased the secretion of IL‐8 and TNF‐α cytokines, and COX‐2 mRNA expression in LPS‐induced THP‐1 macrophages.ConclusionBFL can strengthen the skin barrier function and stimulate skin barrier resistance, to reinforce the skin against oxidative stress and inflammatory stimuli.
Rural‐urban differences in access to care among children and adolescents in the United States
Authors: Crouch Elizabeth , Hung Peiyin , Benavidez Gabriel , Giannouchos Theo , Brown Monique J. 
Published: May 2023

AbstractPurposeRural children and adolescents face disproportionate challenges in access to health care services than their urban counterparts. Yet, recent evidence on disparities in access to health care between rural and urban children and adolescents has been limited. This study examines the associations of residence location with receipt of preventive care, foregone medical care, and continuity of insurance coverage among US children and adolescents.MethodsThis study used cross‐sectional data from the 2019 to 2020 National Survey of Children's Health, with a final sample size of 44,679 children. Descriptive statistics, bivariate analyses, and multivariable logistic regression models were used to examine the differences in preventive care, foregone care, and continuity of insurance coverage between rural and urban children and adolescents.FindingsRural children had lower odds of receiving preventive care (aOR 0.64; 95% CI 0.56‐0.74) and having continuous health insurance coverage (aOR 0.68; 95% CI 0.56‐0.83) compared to urban children. The odds of foregone care were similar between rural and urban children. Children at every federal poverty level (FPL) less than 400% were less likely to receive preventive care, and more likely to forego care than children residing at 400% or above FPL.ConclusionsRural disparities in child preventive care and insurance continuity warrant ongoing surveillance and local access to care initiatives, especially for children in low‐income households. Without updated public health surveillance, policymakers and program developers may not be aware of current disparities. School‐based health centers are 1 avenue for meeting the unmet health care needs of rural children.
Structural and functional properties and digital image texture analysis of gelatin, pectin, and carrageenan gels with honey addition
Authors: Varela María S. , Palacio María A. , Navarro Alba S. , Yamul Diego K. 
Published: May 2023

AbstractGels combined with honey might generate new possibilities of textures in food development. This work explores the structural and functional properties of gelatin (5 g/100 g), pectin (1 g/100 g), and carrageenan (1 g/100 g) gels with different content of honey (0–50 g/100 g). Honey decreased the transparency of gels and made them more yellow‐greenish; all of them were firm and uniform, especially at the highest honey content. The water holding capacity increased (63.30–97.90 g/100 g) and moisture content, water activity (0.987–0.884) and syneresis (36.03–1.30 g/100 g) decreased with the addition of honey. This ingredient modified mainly the textural parameters of gelatin (Hardness: 0.82–1.35 N) and carrageenan gels (Hardness: 2.46–2.81 N), whereas only the adhesiveness and the liquid like‐behavior were increased in the pectin gels. Honey increased the solid behavior of gelatin gels (G': 54.64–173.37 Pa) but did not modify the rheological parameters of the carrageenan ones. Honey also had a smoothing effect on the microstructure of gels as observed in the scanning electron microscopy micrographs. This effect was also confirmed by the results of the gray level co‐occurrence matrix and fractal model's analysis (fractal dimension: 1.797–1.527; lacunarity: 1.687–0.322). The principal component and cluster analysis classified samples by the hydrocolloid used, except the gelatin gel with the highest content of honey, which was differentiated as a separate group. Honey modified the texture, rheology, and microstructure of gels indicating that it is possible to generate new products to be used in other food matrices as texturizers.
Impact melt rocks from the Late Paleocene Hiawatha impact structure, northwest Greenland
Authors: Hyde William R. , Garde Adam A. , Keulen Nynke , Malkki Sebastian N. , Jaret Steven J. , Waight Tod , Beck Pierre , McDonald Iain , Larsen Nicolaj K. 
Published: May 2023

AbstractImpact melt rocks formed during hypervelocity impact events are ideal for studying impact structures. Here, we describe impact melt rock samples collected proximal to the 31 km wide 58 Ma Hiawatha impact structure, northwest Greenland, which is completely covered by the Greenland Ice Sheet. The melt rocks contain diagnostic shock indicators (e.g., planar deformation features [PDF] in quartz and shocked zircon) and form three groups based on melt textures and chemistry: (i) hypocrystalline, (ii) glassy, and (iii) carbonate‐based melt rocks. The exposed foreland directly in front of the structure consists of metasedimentary successions and igneous plutons; however, the carbonate‐based impactites indicate a mixed target sequence with a significant carbonate‐rich component. Well‐preserved organic material in some melt rocks indicates that North Greenland at the time of impact was host to abundant organic material, likely a dense high‐latitude temperate forest. Geochemical signatures of platinum‐group elements in selected samples indicate an extraterrestrial component and support previous identification of a highly fractionated iron impactor in glaciofluvial sand. Our results illustrate the possibility to study impact structures hidden beneath a thick ice sheet based on transported samples and this opens a new avenue for identifying other potential impact craters in Greenland and Antarctica.