bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age-related changes in plasma extracellular vesicles influence neuroinflammation in the brain and neurological outcome after traumatic spinal cord injury
Authors: Lei Zhuofan , Krishnamachary Balaji , Ritzel Rodney M. , Khan Niaz Z. , Li Yun , Li Hui , Brunner Kavitha , Faden Alan I. , Wu Junfang 
Published: April 2023

Abstract

Approximately 20% of all spinal cord injuries (SCI) occur in persons aged 65 years or older. Longitudinal, population-based studies showed that SCI is a risk factor for dementia. However, little research has addressed the potential mechanisms of SCI-mediated neurological impairment in the elderly. We compared young adult and aged C57BL/6 male mice subjected to contusion SCI, using a battery of neurobehavioral tests. Locomotor function showed greater impairment in aged mice, which was correlated with reduced, spared spinal cord white matter and increased lesion volume. At 2 months post-injury, aged mice displayed worse performance in cognitive and depressive-like behavioral tests. Transcriptomic analysis identified activated microglia and dysregulated autophagy as the most significantly altered pathways by both age and injury. Flow cytometry demonstrated increased myeloid and lymphocyte infiltration at both the injury site and brain of aged mice. SCI in aged mice was associated with altered microglial function and dysregulated autophagy involving both microglia and brain neurons. Altered plasma extracellular vesicles (EVs) responses were found in aged mice after acute SCI. EV-microRNA cargos were also significantly altered by aging and injury, which were associated with neuroinflammation and autophagy dysfunction. In cultured microglia, astrocytes, and neurons, plasma EVs from aged SCI mice, at a lower concentration comparable to those of young adult SCI mice, induced the secretion of pro-inflammatory cytokines CXCL2 and IL-6, and increased caspase3 expression. Together, these findings suggest that age alters the EVs pro-inflammatory response to SCI, potentially contributing to worse neuropathological and functional outcomes.
Pharmacological characterisation of store‑operated calcium entry in medulloblastoma cell lines
Authors: Sedeeq Mohammed , Dutta Taush , Maklad Ahmed , Heath John A. , Gueven Nuri , Azimi Iman 
Published: April 2023

Abstract
Store-operated Ca2+ entry (SOCE) is the primary pathway of Ca2+ entry into mammalian cells. Re-modelling of the SOCE pathway has been suggested as the driving mechanism for many tumour phenotypes, such as cancer cell proliferation, migration, and metastasis. Although SOCE has been studied in many cancer types, calcium signalling, especially the SOCE pathway, is largely unexplored in medulloblastoma (MB). MB is the most common malignant paediatric brain tumour, and previously, we reported that some key SOCE components are upregulated in MB. The present study aimed to functionally characterise SOCE in MB cells. Using RT-PCR, the expression of different SOCE-regulating genes was examined cells of different MB subgroups. Our data indicate that specific subgroups of MB cells differentially express SOCE genes. For example, one key regulatory gene, ORAI1, showed a higher expression in the invasive MB subgroups 3. This difference was also reflected by a higher SOCE in these cells compared to cells from MB subgroups associated with lower invasive potential. Overall, the results highlight that distinct MB subgroups rely on differential gene expression that affects their SOCE activity. Future studies will require a functional characterisation to delineate if altered SOCE is causal for the invasiveness of MB, which will be a critical to understand the potential of SOCE as a therapeutic target for the treatment of MB.
Could the Trustworthiness in RAndomized Controlled Trials (TRACT) checklist be trusted?
Authors: Alinany Hesham 
Published: April 2023

Abstract
Randomized controlled trials (RCTs) are considered the gold standard study design in clinical effectiveness research, albeit some RCTs have been found to be fraudulent post-publication. The Trustworthiness in RAndomized Controlled Trials (TRACT) checklist examines RCT integrity through an assessment covering seven domains. While the authors claim that it can identify problematic trials, it has never been validated. A critical appraisal of the checklist highlights major failures in terms of health measurement methodology, including the arbitrary nature of items chosen. We applied the checklist to 16 RCTs coauthored by the checklist developer, and identified potential violations related to retrospective registration, ethical implications, authorship, methodology, implausible timeframes, zero participants to follow-up, implausible baseline characteristics, and excessive inter-group within trial as well as inter-trial similarities. In addition, we identified inconsistencies between the trials’ registration and data in the final published manuscripts. If we uncritically assume that the checklist is valid, then a formal institutional investigation into the trial portfolio its developer is warranted; especially targeting these 16 trials objectively shown to be problematic. The checklist should be considered as neither investigative nor conclusive of potential problems with RCTs until it is further refined and validated.
Biosynthesis and Bioapplications of Nanomaterials from Mushroom Products
Authors: Rai Sachchida Nand , Mishra Divya , Singh Payal , Singh Mohan P. , Vamanu Emanuel , Petre Alexandru 
Published: April 2023

Abstract:
The production of nanoparticles (NPs) from chemical and physical synthesis has ended due to the involvement
of toxic byproducts and harsh analytical conditions. Innovation and research in nanoparticle synthesis
are derived from biomaterials that have gained attention due to their novel features, such as ease of synthesis,
low-cost, eco-friendly approach, and high water solubility. Nanoparticles obtained through macrofungi involve
several mushroom species, i.e., Pleurotus spp., Ganoderma spp., Lentinus spp., and Agaricus bisporus. It
is well-known that macrofungi possess high nutritional, antimicrobial, anti-cancerous, and immune-modulatory
properties. Nanoparticle synthesis via medicinal and edible mushrooms is a striking research field, as macrofungi
act as an eco-friendly biofilm that secretes essential enzymes to reduce metal ions. The mushroom-isolated
nanoparticles exhibit longer shelf life, higher stability, and increased biological activities. The synthesis mechanisms
are still unknown; evidence suggests that fungal flavones and reductases have a significant role. Several
macrofungi have been utilized for metal synthesis (such as Ag, Au, Pt, Fe) and non-metal nanoparticles (Cd,
Se, etc.). These nanoparticles have found significant applications in advancing industrial and bio-medical ventures.
A complete understanding of the synthesis mechanism will help optimize the synthesis protocols and control
the shape and size of nanoparticles. This review highlights various aspects of NP production via mushrooms,
including its synthesis from mycelium and the fruiting body of macrofungi. Also, we discuss the applications
of different technologies in NP high-scale production via mushrooms.
A Comparative Chemoinformatics Analysis of Compounds Extracted from Nyctanthes Arbor-tristis
Authors: Kotharkar Nandini , Bapat Sanket , Vyas Renu , Pathak Pranav 
Published: April 2023

Introduction:
Natural products are a rich source of diverse chemical compounds with
interesting therapeutic properties. There is a need for in-depth investigation of this reservoir with
in-silico tools to assert the molecular diversity with respect to clinical significance. Although studies
have been reported on plants such as Nyctanthes arbor-tristis(NAT) and its medicinal importance.
A comprehensive study on comparative analysis of all phyto-constituents has not been
carried out.

Aim:
n the present work, we have carried out a comparative study of compounds obtained from the
ethanolic extracts of various parts such as calyx, corolla, leaf, and bark of the NAT plant.

Methods:
The extracted compounds were characterized by LCMS and GCMS studies. This was
further corroborated by the network analysis, docking, and dynamic simulation studies with validated
anti-arthritic targets.

Results:
The most significant observation from LCMS and GCMS was that the compounds from
calyx and corolla were closer in chemical space to the anti-arthritic compounds. To further expand
and explore chemical space, the common scaffolds were seeded to enumerate a virtual library. The
virtual molecules were prioritized based on the drug-like, leadlike scores and docked against antiarthritic
targets to reveal identical interactions in the pocket region.

Conclusion:
The comprehensive study will be of immense value to medicinal chemists for the rational
synthesis of molecules as well as bioinformatics professionals for getting useful insight into
identifying rich diverse molecules from plant sources.
Role of Nanomedicines in Controlling Malaria: A Review
Authors: Sharma Sakshi , Rawat Kamla , Bohidar Himadri B. 
Published: April 2023

Abstract:
Malaria has created havoc since time immemorial. It has actually become a major health concern due to its high prevalence in developing countries where poor sanitary conditions facilitate the seasonal breeding of the vector, the female Anopheles mosquito. Even after tremendous ad-vancements in pest control and pharmacology science, managing this disease has not been success-ful, and the cure for this deadly infection has not proven effective lately. The various conventional drugs used are chloroquine, primaquine, mefloquine, atovaquone, quinine, artemisinin etc. All of these have one or other major disadvantages like multi-drug resistance, high dose requirements, ag-gravated toxicity, non-specificity of conventional drugs, and the emergence of drug-resistant para-sites. Therefore, it is necessary to surpass these limitations and look for an alternative to curb the spread of this disease using an emerging technology platform. Nanomedicine is showing promise as an effective alternative tool for the management of malaria. The idea of this tool resonates well with David J. Triggle’s outstanding suggestion “The chemist is as the astronaut, searching for biological-ly useful space in the chemical universe. This review presents a detailed discussion on various nanocarriers, their mode of action and future perspective in treating malaria. Nanotechnology-based drug delivery methods are highly specific, require a lower dose, offer more bioavailability with pro-longed drug release and stay in the body longer. Recent nano drug encapsulation and delivery vehi-cles comprise nanocarriers like liposomes, and organic and inorganic nanoparticles, emerging as promising alternatives for malaria management.
Experiences of Black South African Single Fathers
Authors: Kabongo Tshisuaka E. , Malose Langa 
Published: April 2023

Objective:This study aimed to bridge the gap in the literature on parenting by investigating the experiences of Black single fathers living in Johannesburg, who raise children on their own.Methods:Social constructivism and critical theory served as theoretical frameworks, while narrative interviews were used to collect data. The study involved seven Black male participants, who were selected using a snowball sampling method. Dialogical analysis and Bamberg’s little story approach were used to analyse the data collected.Results:Two themes emerged from the thematic analysis: parenting as a single father and life beyond single fatherhood. The study’s objectives were integrated into the discussion of themes and subthemes that emerged.Conclusion:The study results revealed that, contrary to the common narrative, not all Black fathers are absent. In addition, it pointed to the fact that single fathers are also capable of being effective parents. However, it was discovered that children benefit the most from the active involvement of both parental figures. Implications for these findings and recommendations for future studies were also discussed.
Computational Analysis, In silico Functional Annotation, and Expression of Recombinant PE_PGRS Protein Biomarkers Found in Mycobacterium tuberculosis
Authors: Moodley-Reddy Avanthi , Chiliza Thamsanqa Emmanuel , Pooe Ofentse Jacob 
Published: April 2023

Background:
Over the years, there have been many advances made within the treatment and diagnosis of Mycobacterium Tuberculosis (Mtb). In recent times, the rise of drug resistance has led to higher mortality rates, specifically in poorer countries. There is an urgent need for novel treatment regimens to work against Mtb. Previous studies have identified a gene family within Mtb, known as PE_PGRS proteins, which has shown potential as a drug target. Functional annotations can assist with identifying the role these proteins may play within Mtb.

Methods:
Previous studies indicated PE_PGRS to have potential for further research. The protein biomarkers that showed the most promise were identified as PE_PGRS17, PE_PGRS31, PE_PGRS50, and PEPGRS54. The sequences of these proteins were searched on the Mycobrowser software. Results were designed by entering these sequences into various computational algorithms. PE_PGRS17 showed characteristics of a potential vaccine candidate. Considering this result, expression profiling and purification were conducted on the recombinant PE_PGRS17 Mtb protein biomarker.

Results and Discussion:
The results were calculated using various online software algorithms. Many characteristics were predicted to understand the stability, localization, and function of these proteins. All the proteins have been estimated to produce an immune response or be involved in the process of immunity. The recombinantPE_PGRS17 protein was chosen to be optimally expressed and purified using E.coli as a host cell. These findings specifically on PE_PGRS17, can be expanded in future scientific studies.

Conclusion:
The predicted structures, protein-protein interaction, and antigenic properties of the proteins estimate whether a protein can be used for further studies, specifically as drug/vaccine targets. Ultimately, PE_PGRS17 is seen as the most stable according to its predicted structure, which holds promise as a key factor in future tuberculosis studies.
Revisiting Inhibition Effects of miR-28 as a Metastasis Suppressor in
Gastrointestinal Cancers
Authors: Razi Soofiyani Saiedeh , Minaei Beirami Sohrab , Hosseini Kamran , Mohammadi Nasr Mina , Ranjbar Maryam , Forouhandeh Haleh , Tarhriz Vahideh , Sadeghi Mohammadreza 
Published: April 2023

Abstract:
MicroRNAs are critical epigenetic regulators that can be used as diagnostic, prognostic, and therapeutic biomarkers for the treatment of various diseases, including gastrointestinal cancers, among a variety of cellular and molecular biomarkers. MiRNAs have also shown oncogenic or tumor suppressor roles in tumor tissue and other cell types. Studies showed that the dysregulation of miR-28 is involved in cell growth and metastasis of gastrointestinal cancers. MiR-28 plays a key role in controlling the physiological processes of cancer cells including growth and proliferation, migration, invasion, apoptosis, and metastasis. Therefore, miR-28 expression patterns can be used to distinguish patient subgroups. Based on the previous studies, miR-28 expression can be a suitable biomarker to detect tu-mor size and predict histological grade metastasis. In this review, we summarize the inhibitory effects of miR-28 as a metastasis suppressor in gastrointestinal cancers. miR-28 plays a role as a tumor sup-pressor in gastrointestinal cancers by regulating cancer cell growth, cell differentiation, angiogenesis, and metastasis. As a result, using it as a prognostic, diagnostic, and therapeutic biomarker in the treatment of gastrointestinal cancers can be a way to solve the problems in this field.
Evaluation of an Electronic Care and Rehabilitation Planning Tool With Stroke Survivors With Aphasia: Usability Study
Authors: Davoody Nadia , Eghdam Aboozar , Koch Sabine , Hägglund Maria 
Published: April 2023

Background
Patients with chronic illnesses with physical and cognitive disabilities, particularly stroke survivors with aphasia, are often not involved in design and evaluation processes. As a consequence, existing eHealth services often do not meet the needs of this group of patients, which has resulted in a digital divide.

Objective
The aim of this study was to examine the effectiveness and user satisfaction of an electronic care and rehabilitation planning tool from the perspective of stroke survivors with aphasia. This would help us gain knowledge on how such a tool would need to be adapted for these patients for further development.

Methods
Usability tests were conducted with 9 postdischarge stroke survivors with aphasia. Effectiveness was measured using task-based tests, and user satisfaction was studied through qualitative interviews at the end of each test. All tests were audio recorded, and each test lasted approximately 1 hour. The data were analyzed using qualitative content analysis. As the tool can be used by stroke survivors either independently or with some support from their next of kin or care professionals, the research group decided to divide the participants into 2 groups. Group 1 did not receive any support during the tests, and group 2 received some minor support from the moderator.

Results
The results showed that the care and rehabilitation planning tool was not effective for stroke survivors with aphasia, as many participants in group 1 did not accomplish the tasks successfully. Despite several usability problems and challenges in using the tool because of patients’ disabilities, the participants were positive toward using the tool and found it useful for their care and rehabilitation journey.

Conclusions
There is a need to involve patients with chronic illnesses more in the design and evaluation processes of health information systems and eHealth services. eHealth services and health information systems designed for this group of patients should be more adaptable and flexible to provide them with appropriate functionalities and features, meet their needs, and be useful and easy to use. In addition, the design and evaluation processes should be adapted, considering the challenges of this patient group.
Using Twitter-Based Data for Sexual Violence Research: Scoping Review
Authors: Xue Jia , Zhang Bolun , Zhang Qiaoru , Hu Ran , Jiang Jielin , Liu Nian , Peng Yingdong , Li Ziqian , Logan Judith 
Published: April 2023

Background
Scholars have used data from in-person interviews, administrative systems, and surveys for sexual violence research. Using Twitter as a data source for examining the nature of sexual violence is a relatively new and underexplored area of study.

Objective
We aimed to perform a scoping review of the current literature on using Twitter data for researching sexual violence, elaborate on the validity of the methods, and discuss the implications and limitations of existing studies.

Methods
We performed a literature search in the following 6 databases: APA PsycInfo (Ovid), Scopus, PubMed, International Bibliography of Social Sciences (ProQuest), Criminal Justice Abstracts (EBSCO), and Communications Abstracts (EBSCO), in April 2022. The initial search identified 3759 articles that were imported into Covidence. Seven independent reviewers screened these articles following 2 steps: (1) title and abstract screening, and (2) full-text screening. The inclusion criteria were as follows: (1) empirical research, (2) focus on sexual violence, (3) analysis of Twitter data (ie, tweets or Twitter metadata), and (4) text in English. Finally, we selected 121 articles that met the inclusion criteria and coded these articles.

Results
We coded and presented the 121 articles using Twitter-based data for sexual violence research. About 70% (89/121, 73.6%) of the articles were published in peer-reviewed journals after 2018. The reviewed articles collectively analyzed about 79.6 million tweets. The primary approaches to using Twitter as a data source were content text analysis (112/121, 92.5%) and sentiment analysis (31/121, 25.6%). Hashtags (103/121, 85.1%) were the most prominent metadata feature, followed by tweet time and date, retweets, replies, URLs, and geotags. More than a third of the articles (51/121, 42.1%) used the application programming interface to collect Twitter data. Data analyses included qualitative thematic analysis, machine learning (eg, sentiment analysis, supervised machine learning, unsupervised machine learning, and social network analysis), and quantitative analysis. Only 10.7% (13/121) of the studies discussed ethical considerations.

Conclusions
We described the current state of using Twitter data for sexual violence research, developed a new taxonomy describing Twitter as a data source, and evaluated the methodologies. Research recommendations include the following: development of methods for data collection and analysis, in-depth discussions about ethical norms, exploration of specific aspects of sexual violence on Twitter, examination of tweets in multiple languages, and decontextualization of Twitter data. This review demonstrates the potential of using Twitter data in sexual violence research.
Sistem Peringatan Tingkat Kerentanan Bangunan Berbasis Sensor IMU dengan Metode Fuzzy
Authors: Ahsanandi Muhammad Fikri , Awaludin Lukman 
Published: April 2023

Negara Indonesia merupakan salah satu negara yang memiliki potensi besar terhadap terjadinya gempa bumi. Bangunan yang merupakan salah satu infrastruktur yang sangat penting bagi kehidupan manusia, merupakan sasaran utama bagi bencana alam gempa bumi yang sering terjadi dan dapat menimbulkan kerusakan yang tidak terduga. Oleh karena itu, diperlukan sebuah sistem peringatan yang dapat mengukur dan mengamati getaran yang terjadi dengan besar tertentu untuk mengetahui tingkat kerentanan bangunan tersebut.Sistem ini menggunakan metode logika fuzzy Mamdani dengan proses defuzzyfikasi centroid. Logika fuzzy tersebut digunakan pada sistem peringatan untuk menentukan tingkat bahayanya. Masukan dari sistem terdiri dari nilai resonansi bangunan dan nilai simpangan bangunan. Masukan tersebut diperoleh dari pembacaan sensor IMU MPU6050. Proses defuzzyfikasi menghasilkan nilai keluaran crisp berupa rentang keputusan alarm. Data yang diolah dari pembacaan sensor ditampilkan dalam web server sebagai antarmuka. Berdasarkan hasil pengujian sistem peringatan tingkat kerentanan pada purwarupa bangunan yang telah dilakukan, akurasi logika fuzzy mencapai 95% dari 20 kali pengambilan data. Sistem peringatan yang dirancang dapat berjalan secara real time. Secara keseluruhan proses mulai dari pembacaan sensor hingga akuisisi data dapat berjalan dengan baik.