bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glaucomatocyclitic Crisis - An Elusive Disease: Our Experience in Management and Outcomes
Authors: Parvez S M Masud , Noman Shams Mohammed 
Published: June 2023

Background: The purpose of the current study is to describe clinical manifestations, management and its outcome of patients who were diagnosed as glaucomatocyclitic crisis at the Glaucoma Department Chittagong Eye Infirmary and Training Complex (CEITC) Chattogram and Eye Department, Chittagong Medical College.
Materials and methods: It is a hospital based observational case series study. Study period was from 1st July 2010-to 30th June 2012.Unilateral ocular hypertension cases of unexplained origin referred to glaucoma clinic from outpatient department of CEITC and Eye Department, Chittagong Medical College Hospital (CMCH) were included in this study. Detail history taking and ocular examinations were done that included stitlamp biomicroscopy, applanation tonometry, gonioscopy, fundoscopy and visual field analysis. Glaucomatocyclitic Crisis (GCC) was diagnosed according to mentioned criteria. Management detail was recorded. Patients were followed up after one weak, 1 month, 3 months of initial visit. Examination and investigation findings were documented as much as possible. Sample size was 45.
Results: A total numbers of 45 patients with GCC were included. For the better understanding the results, the total patients (N=45) were categorized into 2 age groups that are 20-49 and 50+. Percentage of patients into these groups are 51.1% (n=23) and 48.9% (n=22) respectively. In total 45 patients, 37 were male and 8 were female. 53.3% patients (n=24) presented with decreased visions, 15.6% patients (n=7) with both pain and occasional redness, 13.3% patients (n=6) with mild discomfort, 11.1% patients (n=5) with recurrent redness, 8.9% patients (n=4) with occasional halos, 4.4% patients (n=2) with occasional eye ache and 2.2% patient (n=1) with loss of vision. Mean IOP of affected eyes was 30mm hg before treatment and 15mm hg after treatment. Angles were open in all cases. At presentation 81.2% patients (n-37) presented with glaucomatous optic disc C:D ratio (0.8-1):1. 77.77% patients(n=35) needed flurometholone, 4.44% cases (n=2) needed dexamethasone and 4.44% patients (n=2) needed prednisolone acetate topical eye drop to control inflammation. 13.33% patients (n=6) were not treated with any steroid as they resolved spontaneously. 91% patients needed anti glaucoma medications 9% needed filtration surgery with Ologen implantation to control IOP.
Conclusion: Early diagnosis with meticulous examinations and investigations are needed to manage a GCC case. As it is an elusive disease, proper explanation of the disorder to the patient to get good compliance from them is necessary to achieve good medical treatment outcome. Good filtration surgery with Ologen implantation is still successful to control IOP. But regular careful monitoring to the patient is mandatory to perfectly manage and prevent the irreversible visual consequences of recurrence of the disease.
JCMCTA 2020 ; 31 (1) : 81-85
Human adenovirus DNA polymerase is evolutionarily and functionally associated with human telomerase reverse transcriptase based on in silico molecular characterization that implicate abacavir and zidovudine
Authors: Fatoki Toluwase Hezekiah 
Published: June 2023

Human adenoviruses (HAdVs) are non-enveloped, small double stranded DNA (dsDNA) viruses that cause asymptomatic infections, clinical syndromes and significant susceptibility to infections in immunocompromised people. The aim of the present study was to identify critical host proteins and HAdV hypothetical proteins that could be developed as potential host-viral targets for antiHAdV therapy. Here, the function of selected hypothetical proteins of HAdV based on phylogenetic relationship with the therapeutic targets of antiretroviral drugs of human immunodeficiency virus (HIV) was predicted computationally, and characterized the molecular dynamics and binding affinity of DNA polymerase of HAdV. Thirty-eight hypothetical proteins (HPs) of human adenovirus (HAdV) were used in this study. The results showed that HAdV DNA polymerase (P03261) is related to Human TERT (O14746) and HLA-B (P01889) genes. The protein-protein interaction of human five molecular targets (PNP, TERT, CCR5, HLA-B, and NR1I2) of ARVDs are well-coordinated/networked with CD4, AHR, FKBP4, NR3C1, HSP90AA1, and STUB1 proteins in the anti-HIV infection mechanism. The results showed that the free energy score of abacavir and zidovudine binding to HAdV DNA polymerase are −5.8 and −5.4 kcal mol-1 respectively. Also, the control drug, cidofovir and ganciclovir have less binding affinity for DNA polymerase of HAdV when compare to that of abacavir and zidovudine. Similarity was observed in the binding of abacavir and zidovudine to HAdV DNA polymerase (ASP742, ALA743, LEU772, ARG773 and VAL776). In conclusion, combination of abacavir and zidovudine was predicted to be potential therapy for controlling HAdV infection targeting HAdV DNA polymerase.
Retinal degeneration in rpgra mutant zebrafish
Authors: Liu Xiliang , Han Shanshan , Liu Fei , Yu Shanshan , Qin Yayun , Li Jingzhen , Jia Danna , Gao Pan , Chen Xiang , Tang Zhaohui , Liu Mugen , Huang Yuwen 
Published: June 2023

Introduction: Pathogenic mutations in RPGRORF15, one of two major human RPGR isoforms, were responsible for most X-linked retinitis pigmentosa cases. Previous studies have shown that RPGR plays a critical role in ciliary protein transport. However, the precise mechanisms of disease triggered by RPGRORF15 mutations have yet to be clearly defined. There are two homologous genes in zebrafish, rpgra and rpgrb. Zebrafish rpgra has a single transcript homologous to human RPGRORF15; rpgrb has two major transcripts: rpgrbex1-17 and rpgrbORF15, similar to human RPGRex1-19 and RPGRORF15, respectively. rpgrb knockdown in zebrafish resulted in both abnormal development and increased cell death in the dysplastic retina. However, the impact of knocking down rpgra in zebrafish remains undetermined. Here, we constructed a rpgra mutant zebrafish model to investigate the retina defect and related molecular mechanism.Methods: we utilized transcription activator-like effector nuclease (TALEN) to generate a rpgra mutant zebrafish. Western blot was used to determine protein expression. RT-PCR was used to quantify gene transcription levels. The visual function of embryonic zebrafish was detected by electroretinography. Immunohistochemistry was used to observe the pathological changes in the retina of mutant zebrafish and transmission electron microscope was employed to view subcellular structure of photoreceptor cells.Results: A homozygous rpgra mutant zebrafish with c.1675_1678delins21 mutation was successfully constructed. Despite the normal morphological development of the retina at 5 days post-fertilization, visual dysfunction was observed in the mutant zebrafish. Further histological and immunofluorescence assays indicated that rpgra mutant zebrafish retina photoreceptors progressively began to degenerate at 3-6 months. Additionally, the mislocalization of cone outer segment proteins (Opn1lw and Gnb3) and the accumulation of vacuole-like structures around the connecting cilium below the OSs were observed in mutant zebrafish. Furthermore, Rab8a, a key regulator of opsin-carrier vesicle trafficking, exhibited decreased expression and evident mislocalization in mutant zebrafish.Discussion: This study generated a novel rpgra mutant zebrafish model, which showed retinal degeneration. our data suggested Rpgra is necessary for the ciliary transport of cone-associated proteins, and further investigation is required to determine its function in rods. The rpgra mutant zebrafish constructed in this study may help us gain a better understanding of the molecular mechanism of retinal degeneration caused by RPGRORF15 mutation and find some useful treatment in the future.
The diagnostic value of electrocardiogram-based machine learning in long QT syndrome: a systematic review and meta-analysis
Authors: Wu Min-Juan , Wang Wen-Qin , Zhang Wei , Li Jun-Hua , Zhang Xing-Wei 
Published: June 2023

IntroductionTo perform a meta-analysis to discover the performance of ML algorithms in identifying Congenital long QT syndrome (LQTS).MethodsThe searched databases included Cochrane, EMBASE, Web of Science, and PubMed. Our study considered all English-language studies that reported the detection of LQTS using ML algorithms. Quality was assessed using QUADAS-2 and QUADAS-AI tools. The bivariate mixed effects models were used in our study. Based on genotype data for LQTS, we performed a subgroup analysis.ResultsOut of 536 studies, 8 met all inclusion criteria. The pooled area under the receiving operating curve (SAUROC) for detecting LQTS was 0.95 (95% CI: 0.31–1.00); sensitivity was 0.87 (95% CI: 0.83–0.90), and specificity was 0.91 (95% CI: 0.88–0.93). Additionally, diagnostic odd ratio (DOR) was 65 (95% CI: 39–109). The positive likelihood ratio (PLR) was 9.3 (95% CI: 7.0–12.3) and the negative likelihood ratio (NLR) was 0.14 (95% CI: 0.11–0.20), with very low heterogeneity (I2 = 16%).DiscussionWe found that machine learning can be used to detect features of rare cardiovascular disease like LQTS, thus increasing our understanding of intelligent interpretation of ECG. To improve ML performance in the classification of LQTS subtypes, further research is required.Systematic Review Registrationidentifier PROSPERO CRD42022360122.
Biochemical association of regulatory variant of KLF14 genotype in the pathogenesis of cardiodiabetic patients
Authors: Alanazi Abdullah Salah , Rasheed Sumbal , Rehman Kanwal , Mallhi Tauqeer Hussain , Akash Muhammad Sajid Hamid , Alotaibi Nasser Hadal , Alzarea Abdulaziz Ibrahim , Tanveer Nida , Khan Yusra Habib 
Published: June 2023

Background and purposeThe study focuses on examining the relationship between a single nucleotide polymorphism (SNP) in KLF14 rs4731702 and risk of type 2 diabetes mellitus (T2DM) and dyslipidemia in different ethnic populations. The purpose of this study was to evaluate the association between KLF14 rs4731702 and serum lipid profile and to determine the frequency distribution of KLF14 rs4731702 among T2DM and cardiometabolic patients.MethodsA total of 300 volunteers were recruited, consisting of three groups: 100 healthy individuals, 100 individuals diagnosed with T2DM, and 100 individuals diagnosed with cardiometabolic disorders. Biochemical analysis of blood samples was conducted to assess various biomarkers related to glycemic control and lipid profile. This involved measuring levels of glucose, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and ApoA1. Genotyping analysis was performed to investigate KLF14 rs4731702 polymorphism. The Tetra ARMS-PCR method was employed for genotyping analysis.ResultsThe results of biochemical profiling revealed a significant association between altered glycemic biomarkers and lipid profile in diseased patients compared to healthy participants. The frequencies of KLF14 rs4731702 alleles and genotypes were compared between the control group and T2DM group. A statistically significant difference was observed, indicating a potential association between KLF14 rs4731702 and T2DM. In the dominant inheritance model of KLF14 rs4731702 SNP, a statistically significant difference [odds ratio (95% confidence interval)] of 0.56 (0.34 –0.96) was found between the control and T2DM subjects. This suggests that the presence of certain genotypes influences the risk of T2DM. In T2DM patients, individuals carrying the C allele exhibited compromised insulin sensitivity, decreased HDL-C and ApoA1 levels, and increased serum glucose, TG, and LDL-C concentrations. Conversely, TT genotype carriers demonstrated increased levels of HDL-C and ApoA1, lower insulin resistance, serum glucose, LDL-C, and TG levels.ConclusionThe study’s findings indicate that dyslipidemia in T2DM patients is associated with reduced KLF14 functionality due to CC and CT genotypes, leading to insulin resistance and an increased risk of cardiovascular diseases. Additionally, risk of KLF14 rs4731702 polymorphism was found to increase with age and was more prevalent in female than in male individuals. These insights contribute to understanding genetic factors influencing the development and progression of T2DM and dyslipidemia in different ethnic populations.
Effect of coffee consumption on thyroid function: NHANES 2007-2012 and Mendelian randomization
Authors: Zhao Guoxu , Wang Zhao , Ji Jinli , Cui Rongjun 
Published: June 2023

BackgroundCoffee is one of the most consumed beverages worldwide, but the effects on the thyroid are unknown. This study aims to examine the association between coffee and thyroid function.MethodsParticipant data (≥ 20 years, n = 6578) for the observational study were obtained from NHANES 2007-2012. Analysis was performed using weighted linear regression models and multiple logistic regression models. Genetic datasets for Hyperthyroidism and Hypothyroidism were obtained from the IEU database and contained 462,933 European samples. Mendelian randomization (MR) was used for the analysis, inverse variance weighting (IVW) was the main method of analysis.ResultsIn the model adjusted for other covariates, participants who drank 2-4 cups of coffee per day had significantly lower TSH concentrations compared to non-coffee drinkers (b=-0.23, 95% CI: -0.30, -0.16), but no statistically significant changes in TT4, FT4, TT3 and FT3. In addition, participants who drank &lt;2 cups of coffee per day showed a low risk of developing subclinical hypothyroidism. (OR=0.60, 95% CI: 0.41, 0.88) Observational studies and MR studies have demonstrated both that coffee consumption has no effect on the risk of hyperthyroidism and hypothyroidism.ConclusionsOur study showed that drinking &lt;2 cups of coffee per day reduced the risk of subclinical hypothyroidism and drinking 2-4 cups of coffee reduced serum TSH concentrations. In addition, coffee consumption was not associated with the risk of hyperthyroidism and hypothyroidism.
The Daily Pattern of Expression of Leptin and Ghrelin O-Acyl Transferase Under Various Lighting Schedules in the Whole Brain of Zebrafish (Danio rerio)
Authors: Mondal Gopinath , Khan Zeeshan Ahmad , Devi Sijagurumayum Dharmajyoti , Labala Rajendra Kumar , Chattoraj Asamanja 
Published: June 2023

Graphical AbstractLight dark cycle influences the levels of Leptin and Ghrelin O-Acyl Transferase (GOAT).
Rheumatoid meningitis: a rare neurological complication of rheumatoid arthritis
Authors: Fan Siyuan , Zhao Jiuliang , Hou Bo , Liu Mange , Niu Jingwen , Zhou Yan , Mao Chenhui , Ren Haitao , Feng Feng , Li Mengtao , Zeng Xiaofeng , Zhu Yicheng , Guan Hongzhi 
Published: June 2023

ObjectiveTo describe the clinical and neuroimaging characteristics of rheumatoid meningitis (RM) in Chinese patients. MethodsThe patients admitted to our hospital with the diagnosis of RM in the past 8 years were retrospectively analyzed. ResultsSix patients with RM were identified among 933 patients admitted with rheumatoid arthritis (RA). The symptoms of meningitis occurred after onset of arthritis in five patients and before onset in one. Headache (n=6), hyperacute focal neurological deficits (n=4) and seizures (n=3) were the most prevalent symptoms. The nadir modified Rankin Scale score was ≥3 in five patients. Rheumatoid factor was elevated in all patients, and interleukin-6 levels in cerebrospinal fluid were dramatically elevated in three of four tested patients. Magnetic resonance imaging of the brain revealed that the meninges were affected in all patients and the cerebral parenchyma was affected in one patient. The lesions were generally located in the frontoparietal region and showed restricted diffusion along the adjacent subarachnoid space. RM occurred during disease-modifying therapy in four patients. In the acute episode, three patients improved on tocilizumab and the other three improved on pulse corticosteroids. For maintenance therapy, two patients received combined therapy of tocilizumab and other immunosuppressive agents, one received adalimumab and methotrexate, and two received low-dose oral corticosteroids with an immunosuppressive agent. Five patients had a good outcome, and one died of Pneumocystis jirovecii pneumonia after stabilization of his neurologic conditions. No relapse of RM occurred on immunotherapy during follow-up. ConclusionsChinese patients with RM share some remarkable clinical and neuroimaging features and respond well to appropriate immunotherapy. Tocilizumab could be a treatment option for this severe complication of RA.
Targeting macrophage Syk enhances responses to immune checkpoint blockade and radiotherapy in high-risk neuroblastoma
Authors: Rohila Deepak , Park In Hwan , Pham Timothy V. , Jones Riley , Tapia Elisabette , Liu Kevin X. , Tamayo Pablo , Yu Alice , Sharabi Andrew B. , Joshi Shweta 
Published: June 2023

BackgroundNeuroblastoma (NB) is considered an immunologically cold tumor and is usually less responsive to immune checkpoint blockade (ICB). Tumor-associated macrophages (TAMs) are highly infiltrated in NB tumors and promote immune escape and resistance to ICB. Hence therapeutic strategies targeting immunosuppressive TAMs can improve responses to ICB in NB. We recently discovered that spleen tyrosine kinase (Syk) reprograms TAMs toward an immunostimulatory phenotype and enhances T-cell responses in the lung adenocarcinoma model. Here we investigated if Syk is an immune-oncology target in NB and tested whether a novel immunotherapeutic approach utilizing Syk inhibitor together with radiation and ICB could provide a durable anti-tumor immune response in an MYCN amplified murine model of NB.MethodsMyeloid Syk KO mice and syngeneic MYCN-amplified cell lines were used to elucidate the effect of myeloid Syk on the NB tumor microenvironment (TME). In addition, the effect of Syk inhibitor, R788, on anti-tumor immunity alone or in combination with anti-PDL1 mAb and radiation was also determined in murine NB models. The underlying mechanism of action of this novel therapeutic combination was also investigated.ResultsHerein, we report that Syk is a marker of NB-associated macrophages and plays a crucial role in promoting immunosuppression in the NB TME. We found that the blockade of Syk in NB-bearing mice markedly impairs tumor growth. This effect is facilitated by macrophages that become immunogenic in the absence of Syk, skewing the suppressive TME towards immunostimulation and activating anti-tumor immune responses. Moreover, combining FDA-approved Syk inhibitor, R788 (fostamatinib) along with anti-PDL1 mAb provides a synergistic effect leading to complete tumor regression and durable anti-tumor immunity in mice bearing small tumors (50 mm3) but not larger tumors (250 mm3). However, combining radiation to R788 and anti-PDL1 mAb prolongs the survival of mice bearing large NB9464 tumors.ConclusionCollectively, our findings demonstrate the central role of macrophage Syk in NB progression and demonstrate that Syk blockade can “reeducate” TAMs towards immunostimulatory phenotype, leading to enhanced T cell responses. These findings further support the clinical evaluation of fostamatinib alone or with radiation and ICB, as a novel therapeutic intervention in neuroblastoma.
Emerging trends on the mechanism of pelvic organ prolapse from 1997 to 2022: visualization and bibliometric analysis
Authors: Yu Xia , Lin Wenyi , Zheng Xuemei , He Li , Yang Zhenglin , Lin Yonghong 
Published: June 2023

ObjectiveAt present, there is no feature description of the mechanism of pelvic organ prolapse (POP) in the literature. This study aimed to map the emerging trends regarding the mechanism of POP from inception to 2022 by bibliometric analysis and to analyze its research hotspots and frontiers.MethodsWe downloaded pertinent publications from inception to 2022 from the Web of Science Core Collection (WoSCC) on 30 June 2022. The data were then examined using the Bibliometrix program in R (Version 4.1.0), CiteSpace software, the Online Analysis Platform of Literature Metrology (https://bibliometric.com), and a bibliometrix online interface.ResultsA total of 290 qualified records on the mechanism of POP were identified and included in the analysis. The most productive journal was International Urogynecology Journal. Bump RC and Olsen AL were the most cited authors. Extracellular matrix, collagen, apoptosis, elastin, oxidative stress, gene expression, matrix metalloproteinase, and tissue engineering were among the 25 most relevant terms. According to the analysis of trending topics, tissue engineering has become a new research hotspot.ConclusionExtracellular matrix remodeling, oxidative stress and apoptosis are the three main directions for studying the mechanism of POP. In addition, tissue engineering has become a new research hotspot. In the future, in-depth research on the interaction between different mechanisms will be carried out, and attempts will be made to combine biomimetic materials and seed cells to achieve the regeneration and reconstruction of POP-related organs.
A study of the correlation between stroke and gut microbiota over the last 20years: a bibliometric analysis
Authors: Han Shengnan , Cai Longhui , Chen Peipei , Kuang Weihong 
Published: June 2023

PurposeThis study intends to uncover a more thorough knowledge structure, research hotspots, and future trends in the field by presenting an overview of the relationship between stroke and gut microbiota in the past two decades.MethodStudies on stroke and gut microbiota correlations published between 1st January 2002 and 31st December 2021 were retrieved from the Web of Science Core Collection and then visualized and scientometrically analyzed using CiteSpace V.ResultsA total of 660 papers were included in the study, among which the United States, the United Kingdom, and Germany were the leading research centers. Cleveland Clinic, Southern Medical University, and Chinese Academy of Science were the top three institutions. The NATURE was the most frequently co-cited journal. STANLEY L HAZEN was the most published author, and Tang WHW was the most cited one. The co-occurrence analysis revealed eight clusters (i.e., brain-gut microbiota axis, fecal microbiome transplantation, gut microbiota, hypertension, TMAO, ischemic stroke, neuroinflammation, atopobiosis). “gut microbiota,” “Escherichia coli,” “cardiovascular disease,” “risk,” “disease,” “ischemic stroke,” “stroke,” “metabolism,” “inflammation,” and “phosphatidylcholine” were the most recent keyword explosions.ConclusionFindings suggest that in the next 10 years, the number of publications produced annually may increase significantly. Future research trends tend to concentrate on the mechanisms of stroke and gut microbiota, with the inflammation and immunological mechanisms, TMAO, and fecal transplantation as hotspots. And the relationship between these mechanisms and a particular cardiovascular illness may also be a future research trend.