bluuebunny/crossref_metadata_2025_split huggingface dataset:
DETERMINANT FACTORS TUBERCULOSIS TRANSMISSION PREVENTION BEHAVIOR AMONG CHILDREN AT KOTABUMI II COMMUNITY HEALTH CENTER, NORTH LAMPUNG, INDONESIA
Authors: Metri Deni , FD Wahyudi 
Published: April 2023

Tuberculosis is one of the causes of death. One of the reasons for that is people’s behavior that does not prevent Tuberculosis transmission. A source of TB transmission in children is adult TB with positive Acid Fast Bacilli test. The behavior of preventing pulmonary TB transmission to children is influenced by predisposing, enabling and reinforcement factors. To prevent the occurrence of transmission is to cut the transmission of these factors off. The purpose was to determine the factors with the behavior of pulmonary TB transmission to children. This study uses an analytical survey with a cross-sectional design. The population is pulmonary TB patients who are married and live with their children, total population. There are 38 respondents which their data are analyzed by unvaried, bivariable and multivariable. In order to complete the data we do a qualitative method by doing deep interviews with 5 health officers, they are head of the community health center, officer of health promotion, surveillance, sanitarian and pulmonary TB program. Factors related to the behavior of preventing the transmission of pulmonary tuberculosis to children are socioeconomic and room ventilation. Education, access to health services, and residential density are factors that are not related to the behavior of preventing the transmission of pulmonary tuberculosis to children. The results of statistical tests show that socioeconomic factor is the most determining factor for the behavior of preventing pulmonary tuberculosis transmission to children. The research implies that seminars, workshops and training for health officers to improve their capacities are needed to help patients dan their families understand the importance of preventing the transmission of tuberculosis to their children
PERCEPTIONS AND READINESS OF THE HEALTH POLYTECHNIC OF THE MINISTRY OF HEALTH RIAU STUDENTS IN IMPLEMENTING IPE-BASED OF INTEGRATED REAL WORK PRACTICE
Authors: Erowati Dewi , Yolahumaroh Yolahumaroh , Aziz Alkausyari 
Published: April 2023

Interprofessional Education (IPE) emphasizes collaborative practice between two or more health professions. The Integrated Real Work Practice of the Health Polytechnic of the Ministry of Health Riau applies the IPE concept, among students of nutrition, midwifery, and nursing. Core competencies for IPE are roles and responsibilities; ethical practice; conflict resolution; communication; and collaboration and teamwork. The purpose of this study was to analyze the relationship between students' perceptions and readiness in implementing the IPE-based of integrated real work practice of the Health Polytechnic of the Ministry of Health Riau. This research is a descriptive study using a cross sectional design in which the measurement of variables was only done once at a time. The sample collection technique was accidental sampling of final year students who attended the integrated real work practice of the Health Polytechnic of the Ministry of Health Riau. The total sample of the study was 230 students, consisting of 76 nutrition students, 63 midwifery students, and 91 nursing students. The instruments used in this study were the Interdisciplinary Education Perception Scale (IEPS) to measure student perceptions and the Readiness for Interprofessional learning Scale (RIPLS) to measure student readiness. The data was carried out in May 2022 and then been analyzed using the Chi Square method to mesure the relationship between the perception and the readiness of the students. The results showed that there was a statistically significant relationship (p = 0.000 or p <0.05) between perceptions and readiness of the Health Polytechnic of the Ministry of Health Riau students in the implementation of IPE-based integrated real work practice. IPE can be implemented successfully and benefits students in real community problems. The program should be evaluated and improved to assure all students from different schools to get equal benefits.
Vascular contributions to the neurobiological effects of prenatal alcohol exposure
Authors: Momin Sarah Z. , Le Jacqueline T. , Miranda Rajesh C. 
Published: April 2023

Background: Fetal alcohol spectrum disorders (FASD) are often characterized as a cluster of brain-based disabilities. Though cardiovascular effects of prenatal alcohol exposure (PAE) have been documented, the vascular deficits due to PAE are less understood, but may contribute substantially to the severity of neurobehavioral presentation and health outcomes in persons with FASD.Methods: We conducted a systematic review of research articles curated in PubMed to assess the strength of the research on vascular effects of PAE. 40 pertinent papers were selected, covering studies in both human populations and animal models.Results: Studies in human populations identified cardiac defects, and defects in vasculature, including increased tortuosity, defects in basement membranes, capillary basal hyperplasia, endarteritis, and disorganized and diminished cerebral vasculature due to PAE. Preclinical studies showed that PAE rapidly and persistently results in vasodilation of large afferent cerebral arteries, but to vasoconstriction of smaller cerebral arteries and microvasculature. Moreover, PAE continues to affect cerebral blood flow into middle-age. Human and animal studies also indicate that ocular vascular parameters may have diagnostic and predictive value. A number of intervening mechanisms were identified, including increased autophagy, inflammation and deficits in mitochondria. Studies in animals identified persistent changes in blood flow and vascular density associated with endocannabinoid, prostacyclin and nitric oxide signaling, as well as calcium mobilization.Conclusion: Although the brain has been a particular focus of studies on PAE, the cardiovascular system is equally affected. Studies in human populations, though constrained by small sample sizes, did link pathology in major blood vessels and tissue vasculature, including brain vasculature, to PAE. Animal studies highlighted molecular mechanisms that may be useful therapeutic targets. Collectively, these studies suggest that vascular pathology is a possible contributing factor to neurobehavioral and health problems across a lifespan in persons with a diagnosis of FASD. Furthermore, ocular vasculature may serve as a biomarker for neurovascular health in FASD.
Automated detection and growth tracking of 3D bio-printed organoid clusters using optical coherence tomography with deep convolutional neural networks
Authors: Bao Di , Wang Ling , Zhou Xiaofei , Yang Shanshan , He Kangxin , Xu Mingen 
Published: April 2023

Organoids are advancing the development of accurate prediction of drug efficacy and toxicity in vitro. These advancements are attributed to the ability of organoids to recapitulate key structural and functional features of organs and parent tumor. Specifically, organoids are self-organized assembly with a multi-scale structure of 30–800 μm, which exacerbates the difficulty of non-destructive three-dimensional (3D) imaging, tracking and classification analysis for organoid clusters by traditional microscopy techniques. Here, we devise a 3D imaging, segmentation and analysis method based on Optical coherence tomography (OCT) technology and deep convolutional neural networks (CNNs) for printed organoid clusters (Organoid Printing and optical coherence tomography-based analysis, OPO). The results demonstrate that the organoid scale influences the segmentation effect of the neural network. The multi-scale information-guided optimized EGO-Net we designed achieves the best results, especially showing better recognition workout for the biologically significant organoid with diameter ≥50 μm than other neural networks. Moreover, OPO achieves to reconstruct the multiscale structure of organoid clusters within printed microbeads and calibrate the printing errors by segmenting the printed microbeads edges. Overall, the classification, tracking and quantitative analysis based on image reveal that the growth process of organoid undergoes morphological changes such as volume growth, cavity creation and fusion, and quantitative calculation of the volume demonstrates that the growth rate of organoid is associated with the initial scale. The new method we proposed enable the study of growth, structural evolution and heterogeneity for the organoid cluster, which is valuable for drug screening and tumor drug sensitivity detection based on organoids.
Bibliometric evaluation of publications on inflammasomes in atherosclerosis from 2002 to 2022
Authors: Tan Yu , Yu Yanqiao , Liu Wei , Ma Xiaojuan , Shi Dazhuo 
Published: April 2023

BackgroundInflammasomes have emerged as an important and promising area of investigation in atherosclerosis. This field, however, lacks bibliometric studies. To help understand how basic and clinical research on inflammasomes in atherosclerosis will develop in the future, we used bibliometric analysis to visualize hotspots and trends.MethodsStudies related to inflammasomes in atherosclerosis were collected from the Web of Science Core Collection database. Each study was analyzed bibliometrically and visually. CiteSpace and VOSviewer software were used to generate knowledge maps.ResultsA total of 894 articles were identified. Sixty-two countries and 338 institutions led by China and the United States contributed to these publications. The leading research institutions were Harvard Medical School and Columbia University. Circulation was the most frequently cited journal in this field. Among the 475 authors determined, Eicke Latz authored the most studies, and Peter Duewell has been cocited the most. NLRP3 inflammasome, NF-kappa B, macrophage and oxidative stress are the most commonly used keywords.ConclusionThere has been a blooming of research on inflammasomes in atherosclerosis during the last two decades. Future studies will likely explore the molecular mechanism of inflammasomes in cell death. More compellingly, researchers may further delve into the potential clinical value of affecting pathological changes in atherosclerosis by modulating the initial transcription immune response and intracellular multiprotein assembly process of the NLRP3 inflammasome. Our research will be helpful to scholars focusing on inflammation—a much-needed breakthrough in the pathophysiological alterations of atherosclerosis—with a novel perspective.
Mega-lake formation in the eastern Hetao Basin, China, during marine isotope stages 7 and 5: A comparison of quartz and feldspar luminescence dating
Authors: Liu JianGang , Fan HaiYan , An Ping , Wen Huan , Yu LuPeng 
Published: April 2023

Paleo-lacustrine sediments indicate that a mega-lake developed in the Hetao Basin (HTB), suggesting dramatic changes in the geomorphology and surface process of the Yellow River and surrounding regions. However, the formation time of the mega Hetao paleo-lake (HTPL) in the HTB remains unclear. In this study, a set of 7.6-m-thick lacustrine sediments in the middle of the HTB is dated by both quartz optically stimulated luminescence (OSL) and K-feldspar (KF) post-infrared infrared stimulated luminescence (p-IR IRSL, pIRIR). The results are as follows. 1) The quartz OSL ages do not increase with depth as the KF pIRIR ages displayed but are saturated at ca. 80–100 ka with the consequence of age underestimation. The influence of signal saturation may occur below 96 Gy (ca. 32 ka) in this region. Thus, the KF pIRIR ages are chosen for geomorphological evolution reconstruction. 2) These ages suggest the level of the HTPL exceeded 1033 m asl twice—at the early stages of MIS 7 (∼255–245 ka) and MIS 5 (∼130–120 ka). However, the exact scale of the HTPL and its relationship with the Jilantai paleo-lake is unclear. 3) This chronology suggests strong linkage between periodic formation of the HTPL and glacial-scale climatic change. We therefore propose an alternative hypothesis to interpret these processes: the Hobq dunefield expanded eastward to dam the narrow Jin-Shaan Gorge during the glacial periods when the flow of the Yellow River was weak. Then, in the beginning of the interglacial periods, the basin was filled by abruptly resumed runoff in response to increased glacial meltwater and precipitation on the northeastern Tibetan Plateau. Once the lake spilt out, the loose dune dam was quickly breached.
Student-centered teacher responses to student behavior in the classroom: A systematic review
Authors: Karasova Jirina , Nehyba Jan 
Published: April 2023

IntroductionEffective communication skills are essential for successful behavior management in the classroom. Teachers who can respond proactively and in a student-centered manner can create a positive and productive learning environment. However, the empirical support for student-centered communication practices in behavior management is limited.MethodsTo address this gap, a systematic literature review was conducted to identify the characteristics of student-centered behavior management strategies that lead to lower behavior problems and increased student engagement. The review utilized a PRISMA protocol to ensure the rigor of the study selection process.ResultsFive main categories were identified that characterize student-centered behavior management responses. A table of 24 communication strategies was presented based on the findings of the review. The study also discussed the further impact of these strategies on student motivation, learning outcomes, responsibility, and interpersonal classroom climate.DiscussionThe findings of this study highlight the importance of effective communication skills in behavior management and provide valuable insights for teachers to improve their practice. By implementing these student-centered communication strategies, teachers can manage the classroom effectively, creating a more positive and productive learning environment and supporting students in achieving better learning outcomes.
A new approach to meteorological observations on remote polar glaciers using open-source internet of things technologies
Authors: Filhol Simon , Lefeuvre Pierre-Marie , Ibañez Juan David , Hulth John , Hudson Stephen R. , Gallet Jean-Charles , Schuler Thomas Vikhamar , Burkhart John F. 
Published: April 2023

Key regions of the world lack sufficient infrastructure to collect geophysical observations, often due to logistical challenges such as difficult accessibility and cost. With the advent of Internet-of-Things (IoT) technologies and low-cost electronics, it is possible today to build monitoring systems collecting spatially distributed, in-situ data with real-time connectivity to online servers for immediate and long-term usage at costs comparable to those of a single autonomous weather station. We present here a custom-built, modular system that collects quality data, and, that is, robust to adverse meteorological conditions and lack of energy. It integrates commercial and custom-built sensors connected to a node (main device) that manages power, data and radio communication. Data is sent to gateways and then to a server that parses, stores and quality controls the data. We deployed two networks in the vicinity of Ny-Ålesund in Svalbard, and operated from May 2021 to April 2022 to measure meteorological and glaciological variables. Our system collected reliable data and had sufficient power resources to survive 4–5 months of darkness during the polar night. Here, we present the design considerations and performance metrics, report our lessons learned from this challenging deployment, and suggest pathways for future improvements.
Comprehensive analysis of a novel RNA modifications-related model in the prognostic characterization, immune landscape and drug therapy of bladder cancer
Authors: Zhang Ziying , Chen Peng , Yun Jingping 
Published: April 2023

Background: Bladder cancer (BCa) is the leading reason for death among genitourinary malignancies. RNA modifications in tumors closely link to the immune microenvironment. Our study aimed to propose a promising model associated with the “writer” enzymes of five primary RNA adenosine modifications (including m6A, m6Am, m1A, APA, and A-to-I editing), thus characterizing the clinical outcome, immune landscape and therapeutic efficacy of BCa.Methods: Unsupervised clustering was employed to categorize BCa into different RNA modification patterns based on gene expression profiles of 34 RNA modification “writers”. The RNA modification “writers” score (RMS) signature composed of RNA phenotype-associated differentially expressed genes (DEGs) was established using the least absolute shrinkage and selection operator (LASSO), which was evaluated in meta-GEO (including eight independent GEO datasets) training cohort and the TCGA-BLCA validation cohort. The hub genes in the RMS model were determined via weighted gene co-expression network analysis (WGCNA) and were further validated using human specimen. The potential applicability of the RMS model in predicting the therapeutic responsiveness was assessed through the Genomics of Drug Sensitivity in Cancer database and multiple immunotherapy datasets.Results: Two distinct RNA modification patterns were determined among 1,410 BCa samples from a meta-GEO cohort, showing radically varying clinical outcomes and biological characteristics. The RMS model comprising 14 RNA modification phenotype-associated prognostic DEGs positively correlated with the unsatisfactory outcome of BCa patients in meta-GEO training cohort (HR = 3.00, 95% CI = 2.19–4.12) and TCGA-BLCA validation cohort (HR = 1.53, 95% CI = 1.13–2.09). The infiltration of immunosuppressive cells and the activation of EMT, angiogenesis, IL-6/JAK/STAT3 signaling were markedly enriched in RMS-high group. A nomogram exhibited high prognostic prediction accuracy, with a concordance index of 0.785. The therapeutic effect of chemotherapeutic agents and antibody-drug conjugates was significantly different between RMS-low and -high groups. The combination of the RMS model and conventional characteristics (TMB, TNB and PD-L1) achieved an optimal AUC value of 0.828 in differentiating responders from non-responders to immunotherapy.Conclusion: We conferred the first landscape of five forms of RNA modifications in BCa and emphasized the excellent power of an RNA modifications-related model in evaluating BCa prognosis and immune landscape.
Maternal tributyrin supplementation in late pregnancy and lactation improves offspring immunity, gut microbiota, and diarrhea rate in a sow model
Authors: Lin Yan , Li Dan , Ma Zhao , Che Lianqiang , Feng Bin , Fang Zhengfeng , Xu Shengyu , Zhuo Yong , Li Jian , Hua Lun , Wu De , Zhang Junjie , Wang Yuanxiao 
Published: April 2023

IntroductionSeveral studies have evaluated the effects of tributyrin on sow reproductive performance; however, none of these studies have investigated the effects of tributyrin on sow gut microbiota and its potential interactions with immune systems and milk composition. Therefore, we speculated that tributyrin, the combination of butyrate and mono-butyrin without odor, would reach the hindgut and affect the intestinal microbiota composition and play a better role in regulating sow reproductive performance, gut flora, and health.MethodsThirty sows (Landrace × Yorkshire) were randomly divided into two groups: the control group (CON) and the tributyrin group (TB), which received basal diet supplemented with 0.05% tributyrin. The experimental period lasted for 35 days from late pregnancy to lactation.ResultsThe results showed that TB supplementation significantly shortened the total parturition time and reduced the diarrhea rate in suckling piglets. On day 20 of lactation, the milk fat and protein levels increased by 9 and 4%, respectively. TB supplementation significantly improved the digestibility of dry material, gross energy, and crude fat in the sow diet, but had no significant effect on crude protein digestibility. Furthermore, TB supplementation increased the levels of IL-10, IL-6, and IgA in the blood of weaned piglets, but had no effect on maternal immunity. Analysis of the fecal microbial composition revealed that the addition of TB during late gestation and lactation increased the microbiota diversity in sows and piglets. At the phylum level, sows in the TB group had a slight increase in the relative abundance of Bacteroidota and Spirochaetota and a decrease in Firmicutes. At the order level, the relative abundance of Lactobacillales was increased in piglets and sows, and the TB group showed increased relative abundance of Enterobacterales and significantly decreased relative abundance of Oscillospirales in piglets. At family level, the relative abundance of Lactobacillaceae, Oscillospiraceae, and Christensenellaceae increased in sows, and the relative abundance of Enterobacteriaceae and Lactobacillaceae increased in piglets. At genus level, the relative abundance of Lactobacillus increased in sows and piglets, but the relative abundance of Subdoligranulum and Eubacterium_fissicatena_group decreased in piglets in the TB group.DiscussionIn conclusion, tributyrin supplementation shortened the farrowing duration and reduced the diarrhea rate of piglets by improving the inflammatory response and composition of gut microbiota in piglets and sows.
The dependent correlation between soil multifunctionality and bacterial community across different farmland soils
Authors: Liu Jing , Gul Wazir Zafran , Hou Guo-Qin , Wang Gui-Zhen , Rong Fang-Xu , Xu Yu-Zhi , Liu Kai , Li Ming-Yue , Liu Ai-Ju , Liu Hong-Liang 
Published: April 2023

IntroductionMicroorganisms play a critical role in soil biogeochemical cycles, but it is still debated whether they influence soil biogeochemical processes through community composition and diversity or not. This study aims to investigate variation in bacterial community structure across different soils and its correlation to soil multifunctionality. Soil samples were collected from five typical farmland zones along distinct climatic gradients in China.MethodsThe high-throughput sequencing (Illumina MiSeq) of 16S rRNA genes was employed to analyze bacterial community composition in each soil sample. Multivariate analysis was used to determine the difference in soil properties, microbial community and functioning, and their interactions.ResultsCluster and discrimination analysis indicated that bacterial community composition was similar in five tested soil samples, but bacterial richness combined with soil enzyme activities and potential nitrification rate (PNR) contributed most to the differentiations of soil samples. Mantel test analysis revealed that bacterial community composition and richness were more significantly shaped by soil nutrient conditions and edaphic variables than bacterial diversity. As for soil multifunctionality, soil microbial community level physiological profiles were little affected by abiotic and biotic factors, while soil enzymes and PNR were also significantly related to bacterial community composition and richness, in addition to soil N and P availability.ConclusionCumulatively, soil enzymes’ activities and PNR were greatly dependent on bacterial community composition and richness not diversity, which in turn were greatly modified by soil N and P availability. Therefore, in the future it should be considered for the role of fertilization in the modification of bacterial community and the consequent control of nutrient cycling in soil.