bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and validation of a prognostic signature for lung squamous cell carcinoma based on metabolism‐related genes
Authors: Wu Mengxi , Yan Jun , Guo Quanwei , Zhang Tao , Tan Jianfeng , Li Dongfang , Kuang Jun , Mo Yijun , Zhang Jianhua 
Published: May 2023

Abstract
Background
Lung squamous cell carcinoma (LUSC) is a main subgroup of non-small cell lung cancer that accounts for ~ approximately 80% of all lung cancer. Studies have demonstrated that tumor microenvironment is essential for the occurrence and development of LUSC. The present study identified the immune- and metabolic-related genes to construct a prognostic model for LUSC patients.
Methods
We downloaded RNA-sequence data and clinical information of LUSC patients from The Cancer Genome Atlas database. The differentially expressed metabolic-related genes (DMRGs) and the differentially expressed immune-related genes (DIRGs) between the control samples and LUSC patient samples were identified. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis were performed to explore the potential biological processes of harvested DMRGs and DIRGs. To develop a prognostic model for LUSC patients, we selected prognostic genes from DMRGs and DIRGs through the univariate and multivariate Cox regression. The prognostic value of the risk model was detected via the Kaplan-Meier curve and the area under the receiver operating characteristic curve analysis. Gene set enrichment analysis (GSEA) was operated to investigate the underlying latent pathways associated with the high-risk group. The infiltration of immune cells in the high- and low-risk groups were analyzed using the single-sample gene-set enrichment analysis.
Results
A total of 178 DMRGs were identified, which were mainly enriched in nucleoside phosphate biosynthetic process, carboxylic acid biosynthetic process, and cofactor metabolic process. Around 264 DIRGs were primarily associated with chemotaxis regulation, leukocyte migration, and positive regulation of cytokine production. After the univariate and multivariate Cox regression analyses, a prognostic risk model containing four prognostic genes was constructed, which performed better in prognosing LUSC patients via the ROC analysis. Moreover, the risk score was found to be an independent prognostic factor through the multivariate Cox regressions analysis. The GSEA suggested that the chemokine signaling pathway, cytokine-cytokine receptor interaction, and IL-17 signaling pathway were mainly enriched in the high-risk group. Finally, the risk score was mostly correlated with immune-related score and immune infiltration cells.
Conclusion
We constructed an immune- and metabolic-related gene signature that was quite reliable in predicting prognosis of LUSC patients.
Major lung resection for carcinoid tumor; Does it affect quality of life?
Authors: Abdeljalil Riad , Abdallah Farah A. , Obeid Zeinab , chaar Mohamad K. Abou , Harb Ahmad Khaled , Shannies Tariq Bassam , El-Edwan Ahed , Haddad Hussam , Ghraibeh Azza , Abu-Shanab Ahmad 
Published: May 2023

Abstract
Background:
Pulmonary carcinoid is rare with surgery remaining the gold standard treatment which may include radical lung resection as well as minimally invasive procedures and parenchymal sparing bronchial resection. However, resection may impact patients' daily activities due to decreased lung volume reserve and postoperative pain. Our study aims to compare the impact of different types of surgical resection on the post-operative quality of life with the application of a strict peri-operative pulmonary care program.
Methods:
Patients who underwent surgery for bronchopulmonary carcinoid tumors in a tertiary cancer center between August, 2017 and March, 2020 were included. For the qualitative and quantitative assessment of pain and quality of life, we utilized the Arabic version of Short-form McGill Pain Questionnaire and Activity of Daily Living (ADL) instrument respectively. Lung reserve was assessed before and after surgery. Statistical analysis conducted on SPSS version 24.
Results:
A total of 16 patients underwent different type of resection. The majority were males (n=10; 63%) with a mean age of 44 years (19-81). Most common clinical stage was stage I (n=12, 75%) with typical carcinoid features recorded in more than half of the cases (n=11, 69%). Almost all patients underwent surgical excision (n=15, 94%) with negative resection margin and no major post-operative complications. Bilobectomy was the most frequent procedure (n=6, 40%) and video-assisted thoracoscopic surgery (VATS) was utilized in 8 patients (50%). Expected changes were recorded in pre- and postoperative pulmonary function test. The majority of patients (n=15, 94%) were totally independent. Mild intermittent pain was found in 7 patients (44%) who scored an average intensity of 1.6 out of 10.
Conclusions:
Excellent long-term outcomes can be achieved following surgical resection of pulmonary carcinoid tumors with little to no effect on patients' lung function and quality of life in regard to performance status and post-operative pain when a good peri-operative pulmonary, physical rehabilitation, and pain management programs are adopted and strictly implemented.
Hydrogels with Tunable Mechanical Plasticity Regulate Endothelial
Cell Outgrowth in Vasculogenesis and Angiogenesis
Authors: Wei Zhao , Lei Meng , Wang YaoHui , Xie Yizhou , Xie Xueyong , Lan DongWei , Jia Yuanbo , Liu Jingyi , Ma Yufei , Chen Bo , Gerecht Sharon , Xu Feng 
Published: May 2023

Abstract
The endothelial cell (EC) outgrowth in both vasculogenesis and angiogenesis starts with remodeling surrounding matrix and proceeds with the crosstalk between cells for the multicelluar vasculature formation. Althought the mechanical plasticity of matrix has been recognized to regulate individual cell behaviors through plastic remodeling, it remains elusive how matrix plasticity impacts cell-to-cell interplay during EC outgrowth and the underlying molecular pathways. Here we developed a collagen-hyaluronic acid based hydrogel platform with tunable plasticity independent of stiffness, by using compositing strategy of dynamic and covalent crosslinks. We show that the increasing plasticity of the hydrogel networks facilitates the matrix remodeling by ECs, thus promoting the process of individual sprouting and branching during vasculogenesis. However, the lagest tubular lumens and the longest invading distance from EC spheoid unexpectedly appear in hydrogels with medium plasticity instead of the highest ones. We unravel that althought the high plasticity of the hydrogels promotes stable integrin cluster of ECs and recruitment of focal adhesion kinase (FAK) with an increase in cell contractility, while the overenhanced contractility downregulates the vascular endothelial calmodulin (VE-CAD) expression and destabilizes the adherens junctions between individual ECs in vasculogenesis (or stalk and tip ECs in angiogenesis). Our results, further validated with mathematical simulations and in vivo angiogenic tests, demonstrate that a balance of matrix plasticity facilitates both cell-matrix binding and cell-to-cell adherens, for promoting vascular assembly and invasion.
Automated cerebral hemorrhage volume calculation and stability detection using automated software
Authors: Sreekrish Anirudh , Venkatasubramanian Chitra , Heit Jeremy J 
Published: May 2023

Abstract
Introduction: The measurement of intracerebral hemorrhage (ICH) volume is important for management, particularly in evaluating expansion on subsequent imaging. However manual volumetric analysis is time-consuming, especially in busy hospital settings. We aimed to use automated Rapid Hyperdensity software to accurately measure ICH volume across repeated imaging.Methods We identified ICH cases, with repeat imaging conducted within 24 hours, from two randomized clinical trials where enrollment was not based on ICH volume. Scans were excluded if there was (1) severe CT artifacts, (2) prior neurosurgical procedures, (3) recent intravenous contrast, or (4) ICH < 1 ml. Manual ICH measurements were conducted by one neuroimaging expert using MIPAV software and compared to the performance of automated software.Results 127 patients were included with median baseline ICH volume manually measured at 18.18 cc (IQR: 7.31–35.71) compared to automated detection of 18.93 cc (IQR: 7.55, 37.88). The two modalities were highly correlated (r = 0.994, p < 0.001). On repeat imaging, the median absolute difference in ICH volume was 0.68cc (IQR: -0.60-4.87) compared to automated detection at 0.68cc (IQR: -0.45-4.63). These absolute differences were also highly correlated (r = 0.941, p < 0.001), with the ability of the automated software to detect ICH expansion with a Sensitivity of 94.12% and Specificity 97.27%.Conclusion In our proof-of-concept study, the automated software has high reliability in its ability to quickly determine IPH volume with high sensitivity and specificity and to detect expansion on subsequent imaging.
Multicenter integrative analysis identifies a multigene signature for lung adenocarcinoma prognosis via glycolysis metabolism discoordination
Authors: Zhang Nannan , Dong Wenlei , Li Junheng , Luo Yuting , Zhu Kunmei , Ren Jie , Li Luyang , Zhang Xinxin , Liu Yixin 
Published: May 2023

Abstract
Background
To explore reliable and reproducible prognostic signatures to aid in guiding clinical decision-making, the present study proposed an integrative analysis method to identify a function-derived gene signature for lung adenocarcinoma (LUAD) prognosis.
Methods
Total 1238 LUAD patients treated with curative resection alone were sourced from public datasets. Using three cohorts of 665 patients in the meta-discovery dataset, we first utilized an integrative analysis method to extract prognostic genes, and identified the essential prognostic genes from a function-derived perspective. Thereafter, we proposed pairwise comparison of single-sample gene set enrichment method to establish a multigene signature for LUAD prognosis based on the function-derived prognostic genes.
Results
Based on integrative analysis, we identified 14 metabolic-related prognostic genes involved in glycolysis metabolism, and established a function-derived signature consisting of these genes for LUAD prognosis (14GM-PS). The prognostic performance of the signature was rigorously validated in two multiple cross-platform independent datasets comprising 299 (log-rank P = 2.78E-06) and 274 (log-rank P = 0.0042) patients, respectively, with significantly different 5-year survival rate. Multivariate Cox analysis demonstrated that the function-derived signature was an independent prognostic factor for LUAD prognosis. Furthermore, the novel proposed nomogram significantly improved the prognostic performance (concordance index) of clinicopathological factors. The molecular and immune characteristics analysis showed that high-risk patients identified by 14GM-PS were characterized by higher hypoxia, proliferation and stemness scores, and lower immune score, providing evidence that could reflect transcriptomic characteristics that are strongly associated with clinical outcomes in the molecular mechanism.
Conclusion
This multicenter study illustrates the accuracy and stability of the function-derived signature for LUAD prognosis, and might become a promising genomic tool to guide individualized application and decision-making of LUAD in clinical practice, with further prospective validation in clinical trials.
Association between dietary folate and migraine in US adults: a cross-sectional study
Authors: Zhu Xiao-Feng , Mo Ye-Tong , Feng Yu-Xue , Liao Wan-Zhe , Wang Lan-Lan , Zheng Cheng , Guo Xu-Guang 
Published: May 2023

Abstract
Background Migraine is a highly prevalent disabling neurological disease that affects 1.04 billion people worldwide. Folate is an essential vitamin B for human neurological health. However, studies between dietary folate and migraines are limited.Methods Data from 9679 participants aged ≥ 20 were collected from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2004. Multivariable logistic regression models, smooth curve fittings, subgroup analyses, and threshold examination were used to check the association between dietary folate and migraines.Results A negative association was exhibited between dietary folate and the prevalence of migraines in the adjusted model. The association between dietary folate and migraines showed a U-shaped curve, with a cutoff point of 1209 mcg. In the stratified analysis, the correlation was subsistent in most subgroups, while no significant interaction effect was found between different subgroups (all P for interaction > 0.05).Conclusion High folate intake was significantly related to a lower odds ratio of migraines. In the future, more studies are needed to confirm our results, while necessary experiments are necessary to explore the exact mechanisms to benefit the treatment and prevention of migraine.
Sterile Insect Technique integration with mass trapping in management of the carob moth, Ectomyelois ceratoniae (Lep.: Pyralidae) in pomegranates
Authors: Soufbaf Mahmoud , Ghorbani Roshanak , Hayatolgheibi Seyed Mohammad Hosein 
Published: May 2023

Abstract
The carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae) is the most important insect pest of pomegranates in Iran. In this research, applicability of the Sterile Insect Technique (SIT) was assessed using three strategies including SIT, SIT + mass trapping (MT) by female sex pheromone, and MT in three isolated orchards in Iran during two consecutive years, 2018 and 2019. Mass rearing of the moths was done in clean rooms on an artificial diet under constant environmental conditions and 200 Gy was applied as the sterilizing dose of gamma irradiation at pupal stage. Insect pest damage was declined in both 2018 and 2019 in all treatments significantly. As a result of SIT in 2018 and 2019, damage of the pest reduced 11.6 and 11.8 times compared to control orchard, respectively. Also, as a result of applying SIT + MT in 2018 and 2019, we observed 2.26 and 2.79 times decrease in the damage relative to the control orchard, respectively. In this way, SIT has been 5.14 and 4.23 times more successful than SIT + MT in 2018 and 2019, respectively. Due to lack of synthetic sex pheromone source, MT treatment was replaced with biological control treatment by Trichogramma embryophagum in 2019 and SIT was more successful than the SIT + parasitoid considerably.
High incidence of metabolic diseases and sedentary lifestyle underline the need for regular counseling in LTFU for CCS- results from a monocentric prospective study
Authors: Richter Franziska , Kronziel Lea Louisa , König Inke Regina , Langer Thorsten , Gebauer Judith 
Published: May 2023

Abstract
Background
Many childhood cancer survivors (CCS) develop treatment-related late effects years after the end of treatment, including increased risk of obesity, metabolic syndrome and related diseases. A healthy lifestyle can reduce the risk of associated comorbidities. Lifestyle counseling during regular long term follow up (LTFU) care could benefit these at-risk CCS.
Methods
Lifestyle counseling was implemented within LTFU care over the study period of one year. Metabolic markers and activity levels were prospectively evaluated as screening parameters for different risk groups. Perspectives of CCS, Physician and sports scientist were compared to identify those in need of counseling. Additionally, a follow-up appointment after one month was performed.
Results
During the counseling offer, 155 CCS (65% female, 35% male) aged between 18 and 63 years (median:30 years) were contacted. Among them, 112 CCS (72%) had an indication for lifestyle counseling based on assessments from physicians, sports scientists, or CCS themselves. 45% were affected by metabolic disorders, 25% met the criteria through BMI and 46% did not meet the recommended activity levels. A total of 120 CCS (77%) received lifestyle counseling, with those who underwent intensive cancer treatment showing the highest need. During the follow-up appointment, CCS indicated high adherence to recommendations and successful implementation into their daily lives.
Conclusion
Metabolic disorders and reduced physical activity impact around two-thirds (68%) of CCS. Nearly all survivors (97%) find the provision of lifestyle counseling during regular LTFU beneficial. Incorporating specialized healthcare professionals into survivorship care enhances the multidisciplinary approach and can aid in promoting a healthy lifestyle, potentially reducing long-term morbidity among CCS.
Study of peanut kernel storage quality discrimination based on odor pattern
Authors: Wang Y.Y , Shi Bolin , Zhao Lei , Tian Shiyi , Zhong Kui , Wang Houyin 
Published: May 2023

Abstract
As part of safety monitoring, checking the quality of peanuts is indispensable. Peanuts contain oils and proteins that are denatured at elevated moisture levels and undergo vigorous metabolism, resulting in mass decay during storage. In this study, the e-nose signals of peanut kernel were classified into three categories of high quality, medium quality and low quality for each storage period by principal component analysis (PCA), which was consistent with the physical and chemical quality clustering results. Using the classification results as learning labels, support vector machine (SVM), decision tree, linear discriminant analysis (LDA) and k-nearest neighbor learning (KNN) were used to develop quality discrimination models for peanut kernels, and the prediction accuracy of each model could reach more than 90%. The results show that the e-nose has an excellent ability to discriminate the quality changes of peanut kernels during storage, in agreement with its classification of physical and chemical quality changes.
Cytokine Levels Reflect Tic Symptoms more Prominently During Mild Phases
Authors: Chi SuHyuk , Mok Young Eun , Kang June , Gim Jeong-An , Han Changsu , Lee Moon-Soo 
Published: May 2023

Abstract
Various cytokines are involved in the neuroinflammatory circuitry of tic disorders. We aimed to identify the cytokines involved in the pathogenesis of tic disorders. We enrolled 44 patients with tic disorder and 38 healthy controls. Blood samples were analyzed using a Luminex® human cytokine multiplex assay kit. Patients were divided into groups with “mild tics” and “above moderate tics” based on Yale Global Tic Severity Scale (YGTSS) scores. In the mild tic group, interleukin (IL)-12 p70 negatively correlated with motor tic scores. Granulocyte-macrophage colony-stimulating factor, IL-4, IL-8, and tumor necrosis factor (TNF)-α were positively correlated to phonic tic scores. IL-12 p40 and TNF-α were positively correlated to total tic scores. IL-12 p70 and IL-17a negatively correlated to impairment scores and total YGTSS scores. Only patients with mild symptoms exhibit significant correlations, suggesting that correlations between cytokines and tic symptoms are more relevant during the mild or remission phases.
Extracorporeal Membrane Oxygenation Treatment for Adults: An Evidence Mapping Based on Systematic Reviews
Authors: Xie Kai , Jing Hui , Guan Shengnan , Kong Xinxin , Ji Wenshuai , Du Shen , Jia Mingyan , Wang Haifeng 
Published: May 2023

AbstractBackground Extracorporeal membrane oxygenation (ECMO) is a cutting-edge life-support treatment measure for patients with severe cardiac and pulmonary illnesses. Although there are several systematic reviews (SRs) about ECMO, it remains to be seen how quality they are and how efficacy and safe the information about ECMO they describe is in these SRs. Therefore, performing an overview of available SRs concerning ECMO is crucial. Methods We searched four electronic databases from inception to January 2023 to identify SRs with or without meta-analyses. The Assessment of Multiple Systematic Reviews 2 (AMSTAR-2) tool, and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system were used to assess the methodological quality, and evidence quality for SRs, respectively. A bubble plot was used to visually display clinical topics, literature size, number of SRs, evidence quality, and an overall estimate of efficacy. Results A total of 17 SRs met eligibility criteria, which were combined into 9 different clinical topics. The methodological quality of the included SRs in this mapping was “Critically low” to “Moderate”. One of the SRs was high-quality evidence, three on moderate, three on low, and two on very low-quality evidence. The most prevalent study used to evaluate ECMO treatment was observational or cohort study with frequently small sample sizes. ECMO treatment has been proven beneficial for severe ARDS and ALI due to the H1N1 influenza infection. For ARDS, ALF or ACLF, and cardiac arrest were concluded to be probably beneficial. For dependent ARDS, ARF, ARF due to the H1N1 influenza pandemic, and cardiac arrest of cardiac origin came to an inconclusive conclusion. No evidence for a harmful association between ECMO treatment and the range of clinical topics. Conclusions There is limited available evidence on treatments for ECMO that large sample, multi-center, and multinational RCTs are needed. Most clinical topics are reported as beneficial or probably beneficial of SRs for ECMO treatment. Evidence mapping is a valuable and reliable methodology to identify and present the existing evidence about therapeutic interventions.
Facile synthesis of lignin Schiff base-copper complex as a catalyst for methylene blue and 4- nitrophenol removal
Authors: Chen Jiansong , Heo Ji Won , Kim Min Soo , Kim Ji Woo , Xia Qian , An Liangliang , Kim Yong Sik 
Published: May 2023

Abstract
Recently, biopolymer-based catalysts have been reported frequently due to the advantages of biodegradability, highly efficiency, and low cost. In this work, novel catalyst lignin Schiff base-copper complex (L-SB-Cu) was synthesized, and this catalyst was used for methylene blue (MB) and 4-nitrophenol (4-NP) reduction in aqueous solutions. The results of characterization from elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), and proton nuclear magnetic resonance (1H NMR) proved that lignin Schiff base (L-SB) was synthesized successfully. X-ray photoelectron spectroscopy (XPS), and EDS-mapping proved that copper ions was coordinated with L-SB, thus forming L-SB-Cu catalyst. The catalyst showed highly catalytic reduction ability towards organic pollutants MB and 4-NP with sodium borohydride (NaBH4) as a reducing agent even after three times of recycling. The catalyst ability of reducing MB and 4-NP was evaluated by UV-Vis spectroscopy, and the mechanisms were explored. This work showed a new strategy of utilizing bio-based material as a highly efficiency catalyst to degrade organic pollutants.
Deep Care: Smart Depression Counselling System through Emotion Recognition and Opinion mining using Deep Learning
Authors: Awate Ashish , Sharma Himanshu , Hatkar Rohit , Shahade Makarand , Nandwalkar Bhushan , Jadhav Yash , Pardeshi Shraddha , Kulkarni Mayuri 
Published: May 2023

Abstract
Depression is a common mental condition that can significantly affect both person’s daily life and mental health. The goal is to create a Depression Detection system for students and patients. The system consists of a depression quiz that takes the opinion of users (Questionnaire) and records a video which is then analyzed to determine the sentiments of patients. In this study, we have used Facial Emotion Recognition – A Kera’s-based Deep Learning model which generates six universal sentiments: angry, sad, disgusted, surprised, fearful, and happy this is then mapped with curated standard questions asked by counsellors. These approaches are then synchronized to produce a depression score which is used to prepare health reports. This health report is sent to the counselor for further treatment.