bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of gene polymorphisms in ACTA2, MYH11, FBN1 and TGF-β signaling with the susceptibility of DeBakey type III aortic dissection
Authors: Zhou Chengming , Zhao Hongwei , Jiang Peipei , Sun Ling , Chang Yafei , Ma Xiang , Du Zhi-Min , Yuan qinghua 
Published: May 2023

Abstract
Object: To evaluate the contribution of actin alpha 2, smooth muscle (ACTA2), myosin heavy chain protein 11 (MYH11), fibrillin 1(FBN1) and transforming growth factor β signaling-related gene polymorphisms and gene-environment interplay in DeBakey type III aortic dissection (AD).
Methods: Twelve single-nucleotide polymorphisms (SNPs) (rs115364997 and rs117593370 of MYH11, rs11070646, rs145233125 and rs201170905 of FBN1, rs2028493, rs2119685 and rs3781211 of ACTA2, rs1800469 of TGFB1, rs1626340 of TGFBR1, rs900 of TGFB2, rs4522809 of TGFBR2) were analyzed in patients with DeBakey III AD (159) and non-AD controls (390). Generalized multifactor dimensionality reduction (GMDR) was used to assess gene-gene and gene-environment interactions.
Results: MYH11 rs115364997 dominant model AG+GG/AA genotype, TGFBR1 rs1626340 dominant model GA+AA/GG genotype and FBN1 rs201170905 dominant model GG+AG genotype and recessive model GG genotype were identified to be associated with AD risk. Also, the TGFB1 rs1800649 G carrier had an increased AD risk. Two SNPs (rs2119685 and rs3781211) located in ACTA2 were also associated with the susceptibility of AD in the dominant model. The gene-gene interaction of FBN1 rs201170905, ACTA2 rs3781211, TGFB1 rs1800469, TGFBR1rs1626340, TGFB2rs900, TGFBR2rs4522809 was identified as the best model([CVC]) 10/10; p =0.0107). The interaction of gene-gene and gene-environment are associated with the risk of DeBakey type III AD.
Role of macrophages in peripheral nervous system involvement in patient with primary Sjogren’s syndrome
Authors: Zheng Jiaman , Zhang Jiayu , Xu Liying , Li Ziqi , Ye Huihao , Yang Min , Yuan Chao 
Published: May 2023

Abstract
Aims: To explore the role of macrophage in peripheral nervous system involvement in patients with primary Sjogren’s syndrome.
Methods and Results:Methods: Sural nerve biopsy was performed in 12 patients diagnosed with primary Sjogren’s syndrome associated peripheral nervous system involvement (pSS-PN). The structural features of sural nerve stained by hematoxylin-eosin(HE) under light microscope. Immunohistochemistry and immunofluorescence staining were carried out to detect the distribution and polarization of macrophage in peripheral neuropathy, using CD206 and iNOS antibodies to label different types of macrophage. The ultrastructural changes of macrophage and peripheral nerves in pSS-PN were observed by transmission electron microscopy. Results: Sural nerve biopsies were performed in 12 patients with pSS-PN, including four men and eight women whose age was 50.4±16.3 years (means±SD) and the duration was 4.43±4.06 months (means±SD). HE staining showed different degrees of reduction of large and small myelinated fibers, NF staining showed various reduction of non-myelinated fibers (mild in 4 cases, moderate in 5 cases, and severe in 3 cases). Immunohistochemistry revealed that inflammatory cell infiltrated in nerve tracts and around blood vessels in 8 cases, and the remaining four patients presented with mixed peripheral neuropathy, mainly axonal damage. Immunofluorescence also revealed that CD68+ macrophages infiltrated in the small vessels and nerve bundles, and M2 macrophages were the main type. Ultrastructure enlarged by electron microscopy showed infiltration of macrophages around nerve fibers, which engulf nerve myelin and axons. In the early stage we can see local swelling and enlarged of axons, then atrophy of axons, and finally separation of axon membrane from myelin, small axis mutation, and loss of normal proportion to myelin.
Conclusions: Macrophages play an important role in Sjogren's syndrome related peripheral neuropathy, and are involved in the occurrence of vasculitis, demyelination and axonal damage.
Interseismic slip distribution and locking characteristics of the mid-southern segment of the Tanlu fault zone
Authors: YU SHUYUAN , LIU ZHUMEI , LIU ZEMIN , LI LAYUE , ZU LIANGYU , YANG YUANYUAN , LUO JIAJI 
Published: May 2023

Abstract

The central and southern sections of the Tanlu fault zone experience frequent seismic activity and have a dense population distribution. Infrastructure, such as high-speed rail and the South–North Water Diversion project, as well as world heritage sites, such as the Grand Canal, ancient city ruins, and cultural landscapes, is located along the fault. A strong earthquake occurring in the future may cause significant social impacts and property losses. It is necessary to clearly understand the current activity characteristics and potential seismic risk of the fault zone to scientifically and reasonably reduce the losses of earthquake disasters. However, there are little data on its current activity through geodetic data. Based on GPS observation data from 1991 to 2018, the fine slip distribution characteristics between present earthquakes in the mid–southern segment of the Tanlu fault zone were retrieved based on the fastest descent method. On this basis, the lock degree and slip rate deficit characteristics of this section were obtained. The differences in the deformation characteristics between the two phases of the mid–southern segment of the Tanlu fault zone were analysed by comparing the surface strain results from 1991 to 2007 and 2013 to 2018. The results show that the interseismic sliding rate of the mid–southern segment of the Tanlu fault zone was 0–1 mm·a-1. The northern Tancheng–Sihong section showed right-handed thrust, and the sliding rate was 0.2–1 mm·a-1. The southern Sihong–Tongcheng section was stretched left–right, and the sliding rate was 0–0.5 mm.a-1 The degree of blockage in the mid–southern segment of the Tanlu fault zone was segmented along the fault strike. In the northern section, the locking depth of the Changyi–Tancheng section was relatively high, with the deepest reaching 32 km, and the maximum compression-slide loss rate reached -1 mm·a-1; the Tancheng–Wangji closure coefficient decreased significantly, with a concentrated distribution between 0.2 and 0.7. The Wangji–Jiashan section has a high degree of closure, small and medium earthquakes are not active, and it is the unruptured section, being more prone to stress accumulation.
Altered Proinflammatory Cytokine Response in Colorectal Cancer Patients: Insights into Immune Dysregulation
Authors: Shakhpazyan Nikolay , Mikhaleva Liudmila , Bedzhanyan Arkady , Sadykhov Nikolay , Midiber Konstantin , Orekhov Alexander 
Published: May 2023

Abstract
Colorectal cancer (CRC) is a significant global health concern and is the third most common cancer worldwide. The interplay between the tumor microenvironment, immune system, and inflammation is critical in CRC development and progression. Pro-inflammatory cytokines, TNF-alpha and IL-1beta, play distinct and contrasting roles in CRC and modulate inflammation in the tumor microenvironment. This study enrolled 12 patients with newly diagnosed colorectal cancer and 9 age- and sex-matched control subjects. Monocyte cultures were obtained from patient blood samples using CD14 + cell isolation and performed Double LPS Stimulation Assay. TNF-alpha and IL-1beta concentrations were measured using ELISA. Our results showed that TNF-α secretion by monocytes in CRC patients became exhausted following the first LPS stimulation, with significantly lower levels after 7 days and no increase after re-stimulation. The secretion of IL-1beta had higher levels in the non-stimulated culture of CRC patients and more pronounced secretion after LPS re-stimulation. These findings suggest an altered pro-inflammatory cytokine response in CRC patients, which could contribute to the tumor microenvironment and inflammatory processes associated with CRC progression. These results provide valuable insights into potential therapeutic targets for modulating the immune response in CRC patients.
CLOCK 3111 T/C SNP Interacts with evening preference, appetite hormones, late eating and sleep reduction for obesity and food intake in obese Iranian adults
Authors: Rahati Sara , Qorbani Mostafa , Naghavi Anoosh , Pishva Hamideh 
Published: May 2023

Abstract

Background
Previous studies have shown that the Circadian locomotor output cycles protein kaput (CLOCK) gene (rs1801260) variant may be associated with obesity risk. Moreover, lifestyle and biochemical parameters have been shown to elicit favorable effects on the obesity risk potentially. Therefore, this study seeks to investigate the effect of lifestyle, biochemical parameters, and CLOCK interaction on food intake and risk of obesity.
Methods
This cross-sectional study comprised 403 overweight and/or obese subjects aged 20–50 from Iran. The CLOCK rs1801260 data was measured by the PCR-RFLP method. Dietary intake, food timing, sleep duration, appetite, and chronotype were assessed by using validated questionnaires. Ghrelin and Glucagon-like peptide-1 (GLP-1) were measured by radioimmunoassay in plasma samples. Participants were also divided into three groups based on rs1801260 genotype. Univariate linear regression models were used to assess the interaction between CLOCK and study parameters on body weight, and logistic regression models were used for interaction terms between CLOCK and study parameters on food intakes.
Results
After controlling confounding factors, our findings showed significant interactions between the C-allele carrier group with chronotype (Pinteraction = 0.048), appetite (Pinteraction = 0.035), lunch time (Pinteraction = 0.016), dinner time (Pinteraction = 0.047), GLP-1 (Pinteraction = 0.035), and ghrelin (Pinteraction = 0.022) on obesity. Also, there was a significant interaction between evening type, high appetite, short sleep and late lunch with C-allele on food intake.
Conclusion
The results of the present study indicate that differences in sleep, appetite hormones, eating behaviors and chronotype influence the risk of obesity differently by CLOCK genotype. These results highlight that diet, gene variants, lifestyle factors, and their interaction should be considered in obesity risk assessment.
WITHDRAWN: Facilitating Positive Outcomes through DREAM Presence Practice Guidelines in Mental Health Nursing
Authors: Chukwuere Precious 
Published: May 2023

Abstract
Background
Mental health nurses are fundamental in facilitating presence for positive nurse and patient outcomes. Presence is a fundamental component of nursing care that focuses on providing comfort, support, and compassion to patients.
Objective
The objective of this study is to facilitate a workshop series on DREAM presence practice guideline (Dedication, Respect through Relationship, Environment, Art of Nursing, and Motivation) due to the dire need for presence practice to improve quality nurse and patient outcomes in South Africa.
Method
As part of a large pre-test and post-test research, a workshop series was organized with nurses caring for adolescents with various mental health illnesses in the two major psychiatric hospitals in the North West Province, South Africa. Access was granted to the study areas after the researchers obtained approval from the Health Research Ethics Committee, North West University (HREC- Reference Number: NWU-00009-22-A1), South Africa, and the North West Province Department of Health (DoH) and the various study areas. Three workshops were conducted in three separate weeks with nurses as frontline health care providers for adolescents with mental illnesses to educate them on DREAM presence practice guidelines.
Results
The study revealed a comprehensive understanding and appreciation of DREAM presence practice guideline by the mental health nurses. Further revealed that mental health nurses want to care and be there for their patients, but they are constrained by various factors such as understaffing, lack of adequate support, poor working conditions, and lack of available resources. Due to the challenges associated with managing adolescents with mental illnesses and the need for presence practice to improve nurse and patient outcomes, there is a dire need to support the nurses working in these wards including adequate psychosocial support, staffing, and funding of the wards to enable nurses to effectively practice presence, which is crucial in achieving positive outcomes. Generally, the study revealed that DREAM presence practice guideline could be the missing link in facilitating quality care in the management of adolescents with mental health illnesses. Participants suggested that mental health nurses setting boundaries during care, watching out for patients’ body language, being patient with the patients, and being open-minded will facilitate DREAM presence practice in the management of adolescents with mental illnesses.
Development and validation of a clinical prognostic model for BRAF V600E mutated colorectal cancer patients based on pathological stage, microsatellite status, and primary tumor site.
Authors: Ou Kai , Liu Xiu , Ma Xiaoting , Yang Lin 
Published: May 2023

Abstract
Objective
To develop and validate a prognostic model for patients with BRAF V600E-mutated colorectal cancer.
Methods
The clinical and pathological information of 206 patients with BRAF V600E mutated colorectal cancer diagnosed in Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College from 2014 to 2021 was retrospectively collected. LASSO regression, COX regression and Nomograms were used to develop clinical prognostic models. The differentiation was measured by C-statistic and the predicted variability was evaluated by calibration curve. The prognostic model was externally validated with validation set data from 164 patients pooled from 5 studies.
Results
Our clinical prognostic model included three variables: pathological stage, microsatellite status, and primary tumor site. In internal validation, the model had a concordant index of 0.785 (95%CI [0.732–0.839]) and a concordant index of 0.754 (95%CI [0.698–0.810]) using pathological staging. External validation confirmed the robustness of the model with a consistency index of 0.670 (95%CI ([0.617–0.724]) and a consistency index of 0.584 (95%CI [0.546–0.622]) using pathological staging. The calibration graph drawn based on the prediction and the actual situation is close to the 45° diagonal.
Conclusion
By adding microsatellite status and primary tumor site on the basis of pathological stage, we improved the discriminability and prediction accuracy of the model, and successfully established a prognosis model for patients with BRAF V600E mutation of colorectal cancer.
UV-Rays induced Cytomorphological and biochemical variations in Stevia rebaudiana Bertoni
Authors: Kumar Girjesh , Tiwari Naveen Kumar , Pandey Satya , Pandey Priyanka , Yadav Jyoti 
Published: May 2023

Abstract

Stevia rebaudiana Bertoni is a perennial herb of the Asteraceae family and is highly medicinal in number of ways. Abiotic stress such as UV radiation exposure was reported to have a beneficial effect on morphological traits of the plant. Plantlets of Stevia (4–5 cm) were exposedto UV-B rays with differential timeviz. 20, 40, 60, 80, and 100 minutes, respectively along withthe control set. The exposed plantlets were transferred to the greenhouse for further study and growth. After 45 days of plantation, morphological studies were taken out and tabulated. At intermediate doses i.e., 40 and 60 min, an increase in leaf area, leaf width, leaf length, plant height, internodal length and width of stem have been found in relation to morphological traits while in case of cytological study different chromosomal arrangements were observed viz. scattering, precocious movement, stickiness, unorientation and bridge formation,etc. Hence on the basis of morphological and cytological studies performed on Stevia afterUV irradiation,it was concluded that, shorter exposure of UV-B(40 and 60 min)induced some better morphological traits so that the qualitative and quantitative features of the plant were enhanced.
An efficient synthesis of phosphonated cyclopentenones by NaN 3 -catalyzed three-component reaction between trialkyl phosphites, ethyl arylmethylidenecyanoacetates and dialkyl acetylenedicarboxylates
Authors: Derranjia Akram Ranjbar , Anary-Abbasinejad Mohammad 
Published: May 2023

Abstract
NaN3-catalysed three-component reaction between trialkyl phosphites, dialkyl acetylenedicarboxylates and ethyl arylmethylidenecyanoacetates afforded phosphonated cyclopentenone derivatives. The process involves one C-P and two C-C bond formations in one synthetic step. All reactions were conducted in acetone as solvent at room temperature and the products were obtained in high yields as stable solids. The products were isolated and purified by simple washing with water and diethyl ether without need to tedious chromatography methods. The structures of products were proved by 1H, 13C and 31P NMR and IR spectral and elemental analysis data.
Risk Prediction of Second Primary Malignancies in Patients after Rectal Cancer: Analysis Based on SEER Program
Authors: Sun Yong-Chao , Zhao Zi-Dan , Fu Yue , Jiao Yu-Wen , Zhang Jia-Wen , Shi Wei-Hai 
Published: May 2023

Abstract
Background This study will focus on exploring the clinical characteristics of rectal cancer (RC) patients with SPMs and constructing a prognostic nomogram to provide clinical treatment decisions.Methods We determined the association between risk factors and overall survival (OS) while establishing a nomogram to forecast the further OS status of these patients via Cox regression analysis. Finally, we evaluated the performance of the prognostic nomogram to predict further OS status.Results Nine parameters were identified to establish the prognostic nomogram in this study, and, the C-index of the training set and validation set was 0.691 (95%CI, 0.662–0.720) and 0.731 (95%CI, 0.676–0.786), respectively.Conclusions We systematically explored the clinical characteristics of SPMs after RC and constructed a satisfactory nomogram.
Direct regulation of the cardiac ryanodine receptor (RyR2) by O-GlcNAcylation
Authors: Okolo Chidinma A. , Khaing Ei-Phyo , Mereacre Valeria , Munro Michelle L , Erickson Jeffery R , Jones Peter P. 
Published: May 2023

Abstract
Background
O-GlcNAcylation is the enzymatic addition of a sugar, O-linked β-N-Acetylglucosamine, to the serine and threonine residues of proteins, and is abundant in diabetic conditions. We have previously shown that O-GlcNAcylation can trigger arrhythmias by indirectly increasing pathological Ca2+ leak through the cardiac ryanodine receptor (RyR2) via Ca2+/calmodulin-dependent kinase II (CaMKII). However, RyR2 is well known to be directly regulated by other forms of serine and threonine modification, therefore, this study aimed to determine whether RyR2 is directly modified by O-GlcNAcylation and if this also alters the function of RyR2 and Ca2+ leak.
Methods
O-GlcNAcylation of RyR2 in diabetic human and animal hearts was determined using western blotting. O-GlcNAcylation of RyR2 was pharmacologically controlled and the propensity for Ca2+ leak was determined using single cell imaging. The site of O-GlcNAcylation within RyR2 was determined using site-directed mutagenesis of RyR2.
Results
We found that RyR2 is modified by O-GlcNAcylation in human, animal and HEK293 cell models. Under hyperglycaemic conditions O-GlcNAcylation was associated with an increase in Ca2+ leak through RyR2 which persisted after CaMKII inhibition. Conversion of serine-2808 to alanine prevented an O-GlcNAcylation induced increase in Ca2+ leak.
Conclusions
These data suggest that the function of RyR2 can be directly regulated by O-GlcNAcylation and requires the presence of serine-2808.
Demethylation in promoter region of severely damaged hepatocytes enhances chemokine receptor CXCR4 gene expression
Authors: Ito Chihiro , Haraguchi Ryuma , Ogawa Kohei , Iwata Miku , Kitazawa Riko , Takada Yasutsugu , Kitazawa Sohei 
Published: May 2023

Abstract
The liver is known to possess remarkable regenerative potential, but persistent inflammation or severe acute injury can lead to liver fibrosis and incomplete regeneration, ultimately resulting in liver failure. Recent studies have shown that the axis of two types of CXCL12 receptors, CXCR4 and CXCR7, plays a crucial role in liver fibrosis and regeneration. The present study is aimed at investigating the regulatory factors involved in CXCR4 expression in injured liver. Immunohistochemical screening of liver tissue samples collected during liver transplantation revealed a reciprocal expression pattern between CXCR4 and MeCP2. An in vitro system involving cultured cell lines and H2O2 treatment was established to study the impact of oxidative stress on signaling pathways and epigenetic alterations that affect CXCR4 mRNA expression. Operating through distinct signaling pathways, H2O2 treatment induced a dose-dependent increase in CXCR4 expression in both hepatocyte- and intrahepatic cholangiocyte-derived cells. Treatment of the cells with trichostatin and azacytidine modulated CXCR4 expression in hepatocytes by modifying the methylation status of CpG dinucleotides located in a pair of TA repeats adjacent to the TATA-box of the CXCR4 gene promoter. Only MeCP2 bound to oligonucleotides representing the TATA-box region when the cytosine residues within the sequence were methylated, as revealed by Electrophoretic Mobility Shift Assay (EMSA). Methylation-specific PCR analysis of microdissected samples revealed a correlation between the loss of CpG methylation and the upregulation of CXCR4 in injured hepatocytes, replicating the findings from the in vitro study. Besides the conventional MEK/ERK and NF-κB signaling pathways that activate CXCR4 in intrahepatic cholangiocytes, the unique epigenetic modifications observed in hepatocytes might also contribute to a shift in the CXCR4-CXCR7 balance towards CXCR4, leading to irreversible liver injury and fibrosis. This study highlights the importance of epigenetic modifications in regulating CXCR4 expression in liver injury and fibrosis.
Performance evaluation and ranking of CMIP6 global climate models over Vietnam
Authors: Nguyen-Duy Tung , Ngo-Duc Thanh , Desmet Quentin 
Published: May 2023

Abstract

This study comprehensively assesses the performance of 29 Coupled Model Intercomparison Project Phase 6 (CMIP6) Global Climate Models (GCMs) and their ensemble mean (ENS_MEAN) over Vietnam. The spatiotemporal variability of near-surface temperature and precipitation is thoroughly evaluated for the 30-year historical period of 1985–2014. Results show that the models can reasonably reproduce the observational annual cycles and spatial distribution of temperature and precipitation, though their performances vary across the seven climatic sub-regions of Vietnam. Due to their coarse resolutions, the models produce warm biases over certain highland and mountainous areas, and many of them cannot reproduce the rainy season shift from summer in most sub-regions toward the end of the year in Central Vietnam. Finally, these results are summarized, and a performance score is assigned to each model, inferring a ranking. The top three models are EC-Earth3-Veg, EC-Earth3, and HadGEM3-GC31-MM. Although created by simply averaging all the models, ENS_MEAN can compete with the best GCMs and ranks 4th overall. In the same model family, the higher-resolution experiment exhibits a higher ranking than the lower-resolution one. The results of this study can provide a reference for the choice of appropriate CMIP6 GCMs in the upcoming downscaling activities in Vietnam.