bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbon source priority and availability limit bidirectional electron transfer in freshwater mixed culture electrochemically active bacterial biofilms
Authors: Michalska Karina , Brown Robert Keith , Schröder Uwe 
Published: September 2023

AbstractThis study investigated, if a mixed electroactive bacterial (EAB) culture cultivated heterotrophically at a positive applied potential could be adapted from oxidative to reductive or bidirectional extracellular electron transfer (EET). To this end, a periodic potential reversal regime between − 0.5 and 0.2 V vs. Ag/AgCl was applied. This yielded biofilm detachment and mediated electroautotrophic EET in combination with carbonate, i.e., dissolved CO2, as the sole carbon source, whereby the emerged mixed culture (S1) contained previously unknown EAB. Using acetate (S2) as well as a mixture of acetate and carbonate (S3) as the main carbon sources yielded primarily alternating electrogenic organoheterotropic metabolism with the higher maximum oxidation current densities recorded for mixed carbon media, exceeding on average 1 mA cm−2. More frequent periodic polarization reversal resulted in the increase of maximum oxidative current densities by about 50% for S2-BES and 80% for S3-BES, in comparison to half-batch polarization. The EAB mixed cultures developed accordingly, with S1 represented by mostly aerobes (84.8%) and being very different in composition to S2 and S3, dominated by anaerobes (96.9 and 96.5%, respectively). S2 and S3 biofilms remained attached to the electrodes. There was only minor evidence of fully reversible bidirectional EET. In conclusion the three triplicates fed with organic and/or inorganic carbon sources demonstrated two forms of diauxie: Firstly, S1-BES showed a preference for the electrode as the electron donor via mediated EET. Secondly, S2-BES and S3-BES showed a preference for acetate as electron donor and c-source, as long as this was available, switching to CO2 reduction, when acetate was depleted.
Graphical Abstract
Fibrolamellar hepatocellular carcinoma: a case report and gene analysis
Authors: Watanabe Akira , Harimoto Norifumi , Saito Hideyuki , Kawabata-Iwakawa Reika , Seki Takaomi , Muranushi Ryo , Hoshino Kouki , Hagiwara Kei , Ishii Norihiro , Tsukagoshi Mariko , Igarashi Takamichi , Araki Kenichiro , Ikota Hayato , Ishige Takashi , Mimori Koshi , Shirabe Ken 
Published: September 2023

Abstract
Background
Fibrolamellar hepatocellular carcinoma (HCC) (FL-HCC) is rare in Japan. FL-HCC develops in young patients with no history of cirrhosis and tends to manifest lymphatic metastasis with clinical features similar to those of HCC. We present a case of FL-HCC in a young male patient.

Case presentation
A 14-year-old male patient underwent abdominal computed tomography (CT) to diagnose appendicitis, wherein a hepatic tumor was detected. Dynamic enhanced CT revealed a 35-mm solid tumor, which contrasted at the early phase of dynamic enhanced study of the right hepatic segments, with occlusion of the right portal vein. We performed right hepatectomy for these lesions. The patient experienced a single lymphatic recurrence on the hepatoduodenal ligament 12 months after the initial surgery. We performed lymphadenectomy for the recurrent tumor. We performed RNA sequencing (RNA-seq) and targeted DNA sequencing of the resected specimens (primary tumor, lymphatic metastasis, and normal liver). RNA-seq detected DNAJB1-PRKACA in both primary and metastatic lesions as previously reported. Furthermore, The Cancer Genome Atlas (TCGA) database was used to compare other gene expressions in this case with those of previously reported cases of FL-HCC and HCC in young patients. Principal component analysis of differentially expressed genes in the top 10% revealed that the gene expression in our case was similar to that of previous FL-HCC cases but was a different cluster from that in HCC cases in young patients. Mutational analysis did not detect any somatic mutations associated with carcinogenesis, including previously reported mutations (Kastenhuber et al. in Proc Natl Acad Sci USA 114: 13076–84, 2017).

Conclusion
We encountered a case of FL-HCC, a rare hepatic tumor in an adolescent patient, and evaluated the genetic background. Our findings could contribute to the elucidation of the mechanisms underlying carcinogenesis and progression in patients with FL-HCC and thereby contribute to the development of new therapeutic strategies in the future that may improve patient prognosis.
Complete heart block is a significant predictor of mortality in immune checkpoint inhibitor myocarditis
Authors: O’Shea Michael P. , Karikalan Suganya Arunachalam , Yusuf Ali , Barry Timothy , Habib Eiad , O’Shea John , Killian Michael , Baqal Eman , Nayak Srishti , Masson Rajeev , Hermann Joerg , Shah Shimoli , Ayoub Chadi , Masry Hicham El 
Published: September 2023

Abstract
Background
Immune checkpoint inhibitor (ICI) myocarditis is associated with significant mortality risk. Electrocardiogram (ECG) changes in ICI myocarditis have strong prognostic value. However the impact of complete heart block (CHB) is not well defined. This study sought to evaluate the impact of CHB on mortality in ICI myocarditis, and to identify clinical predictors of mortality and CHB incidence.

Methods
We conducted a retrospective cohort study of patients with ICI myocarditis at three Mayo Clinic sites from 1st January 2010 to 31st September 2022 to evaluate mortality rates at 180 days. Clinical, laboratory, ECG, echocardiographic, and cardiac magnetic resonance imaging (CMR) characteristics were assessed. Cox and logistic regression were performed for associations with mortality and CHB respectively.

Results
Of 34 identified cases of ICI myocarditis, 7 (20.6%) had CHB. CHB was associated with higher mortality (HR 7.41, p = 0.03, attributable fraction 86.5%). Among those with CHB, troponin T (TnT) < 1000 ng/dL, low white blood cell count and high ventricular rate at admission were protective. There was trend towards increased survival among patients who underwent permanent pacemaker insertion (p = 0.051), although most experienced device lead complications. Factors associated with development of CHB included prolonged PR and QRS intervals and low Sokolow Lyon Index. Where these were normal and TnT was < 1000 ng/dL, no deaths occurred. Impaired myocardial longitudinal strain was sensitive for ICI myocarditis but was not prognostically significant.

Conclusion
There is a strong temporal association between CHB and early mortality in people with ICI myocarditis. Focusing on arrhythmogenic complications can be helpful in predicting outcomes for this group of critically ill individuals.
Head lice as vectors of pathogenic microorganisms
Authors: Feldmeier Hermann 
Published: September 2023

AbstractBody lice and head lice are the most common ectoparasites of humans. Head lice (Pediculus humanus capitis) occur worldwide in children and their caretakers, irrespective of their social status. In contrast, body lice (Pediculus humanus corporis) are confined to marginalized population groups in countries of the Global South, homeless people, and refugees. Body lice are known to transmit an array of bacterial pathogens, such as R. prowazekii, R. rickettsii, C. burneti, B. quintana, B. recurrentis, and Y. pestis. The vector capacity of head lice is still a matter of debate. The objective of the review was to scrutinize the existing evidence on the vector capacity of head lice for the transmission of bacterial pathogens. The PUBMED database was searched using a combination of the terms “pediculus humanus” OR “body lice” OR “head lice” AND “pathogen” OR “Rickettsia prowazekii” OR “Bartonella quintana” OR “Borrelia recurrentis” OR “Coxiella burneti” without a time limit. Data from epidemiological studies as well as historical observations demonstrate that body lice and head lice can carry the same array of pathogens. Since the presence of a bacterial pathogen in an arthropod is not sufficient to state that it can be transmitted to humans, and since experimental models are lacking, as yet one cannot conclude with certainty that head lice serve as vectors, although this review presents circumstantial evidence that they do. Adequately designed experimental and epidemiological studies are needed to ascertain the exact transmission potential of head lice.
Effectiveness and cost-effectiveness of a multimodal, physiotherapist-led, vocational intervention in people with inflammatory arthritis: study protocol of the Physiotherapy WORKs trial
Authors: Bakker N. F. , van Weely S. F. E. , Hutting N. , Heerkens Y. F. , Engels J. A. , Staal J. B. , van der Leeden M. , Boonen A. , van den Hout W. B. , Vliet Vlieland T. P. M. , Knoop J. 
Published: September 2023

Abstract
Background
Although reduced work ability is a substantial problem among people with inflammatory arthritis (IA), work ability is an underexposed area in clinical practice. Evidence on vocational interventions in IA is limited, but favourable results of delivery by a physiotherapist (PT) warrant the need for further research. Therefore, we aim to evaluate the (cost-)effectiveness of a multimodal, PT-led, vocational intervention in (self-)employed people with IA compared to usual care.

Methods
This randomized controlled trial will include 140 people with rheumatoid arthritis (RA) or axial spondyloarthritis (axSpA) who are (self-)employed and have reduced work ability (Work Ability Index – Single Item Scale (WAS) ≤ 7/10) and/or RA/axSpA related sick leave (≤ 6 months). Participants will be randomized 1:1 to the intervention or control condition (usual care). The intervention, delivered by primary care PTs, will be personalized to each patient, consisting of 10 to 21 sessions over 12 months. The intervention will be multimodal, comprising of 1) exercise therapy and a physical activity plan, 2) education/self-management support, 3) work-roadmap to guide participants in finding relevant other care, with optionally 4) online self-management course and 5) workplace examination. Assessments will be performed at baseline and after 3, 6, and 12 months. The primary outcome measure of effectiveness is work ability, as measured with the WAS at 12 months. For the cost-effectiveness analysis, the EuroQol (EQ-5D-5L), self-reported healthcare use, sick leave and productivity while at work will be used to estimate the trial based cost-utility from a societal perspective. A process evaluation, including assessments of adherence and treatment fidelity, will be undertaken using the registrations of the PTs and semi-structured interviews at 12 months follow-up in a random sample of the intervention group.

Discussion
The results of this study will provide insights in the (cost-)effectiveness of a multimodal, PT-led, vocational intervention in people with IA and a reduced work ability.

Trial registration
This study is registered in the International Clinical Trial Registry Platform (ICTRP) under number NL9343.
Achievement of pathological complete response with osimertinib for EGFR-mutated lung adenocarcinoma
Authors: Fujita Yasuhiro , Take Nobuyuki , Shinohara Shinji , Mori Masataka , Kanayama Masatoshi , Takenaka Masaru , Kuroda Koji , Shimajiri Shohei , Tanaka Fumihiro 
Published: September 2023

Abstract
Background
Tyrosine-kinase inhibitors (TKIs) of epidermal growth factor receptor (EGFR) usually provide a potent anti-tumor efficacy with robust radiographic response for non-small cell lung cancer (NSCLC) harboring activating mutations in the EGFR gene. However, first-generation EGFR-TKIs may provide only modest pathological response in the majority of EGFR-mutated NSCLC. Here, we present a case of EGFR-mutated adenocarcinoma in which a pathological complete response was revealed in histological specimens obtained during conversion surgery following systemic treatment using a third-generation EGFR-TKI.

Case presentation
A 61-year-old Japanese man was admitted to our hospital for salvage surgery. Four months prior to the admission, he had been diagnosed with unresectable adenocarcinoma (clinical stage IIIA/T4N1MO) originating from the right lower lobe. Chest computed tomography had revealed a 3.4-cm tumor and enlarged hilar nodes that invaded into the left atrium through the lower pulmonary vein. An activating EGFR-mutation (L858R) had been detected in the tumor specimen. Osimertinib monotherapy had provided a dramatic radiographic response. The patient was diagnosed with potentially resectable disease after 4 months’ osimertinib treatment and was referred to our hospital. Complete resection with right lower lobectomy and combined resection of the left atrium was achieved. Pathological examination showed no viable tumor cells in the resected specimens. Postoperative course was uneventful. The patient is alive without tumor recurrence at 2 years after surgery.

Conclusions
Pathological complete response was achieved with systemic treatment with osimertinib prior to surgery. Conversion surgery after osimertinib treatment may be safe and effective for NSCLC harboring activating EGFR-mutations.
Clinical judgement of general practitioners: an effective tool in the diagnosis of dementia?
Authors: Rajgopal Aditi , Singh Gracy 
Published: September 2023

SUMMARYThe diagnosis of dementia poses several challenges, as a consequence of which the condition has been widely reported to be underdiagnosed in the general population. Currently, there is no single diagnostic test for dementia and the clinical judgement of primary care physicians is therefore a key determinant in identifying which patients are referred to specialist services for further assessment. This month's Cochrane Corner review found that the clinical judgement of general practitioners is more specific (58–99%) than sensitive (34–91%) in diagnosing dementia, although the data were limited by small sample size and significant heterogeneity. This commentary provides a critical appraisal of this systematic review and attempts to extrapolate conclusions relevant to current clinical practice, including potential areas of further research, to facilitate appropriate and timely referral of patients with suspected dementia to specialist services.
Sexuality and Demographic Change: Documenting Family Formation Trajectories and Cohort Change in the LGB Population
Authors: Ophir Ariane , Boertien Diederik , Vidal Sergi 
Published: September 2023

Abstract
Narratives of demographic shifts overlook how societal changes shape the family trajectories of sexual minorities. Using sequence analysis, we describe how partnering and parenthood evolve over the life course of lesbian, gay, and bisexual (LGB) women and men in the United Kingdom (N = 455) and how the types of these family trajectories changed across two birth cohorts (born before 1965 and in 1965–1979). We find five distinct trajectories between ages 18 and 40, wherein two thirds of the sample belonged to a family trajectory that did not involve living with children. Partnership-centered trajectories became more common across cohorts, and this increase came at the expense of trajectories characterized by singlehood among gay men and lesbian women. However, parenthood trajectories became less common among all LGB groups. Furthermore, family trajectories became more complex across cohorts, including more transitions, which coincides with trends in the general population. Yet we also find that family trajectories became less diverse among lesbian women and bisexual men, in contrast to the trend among gay men and the general population. The results demonstrate the dynamic, complex, and diverse nature of LGB individuals' family lives and why existing narratives of family-related demographic change should explicitly consider sexual minorities in demographic narratives.
Contesting Chinese Contract Labor: Yung Wing's Reports and the Qing Mission to Peru
Authors: Tinsman Heidi 
Published: September 2023

Abstract
In 1874, government officials in Qing China sent a Yale-educated special envoy named Yung Wing to Peru to survey the conditions of Chinese contract workers on plantations. A parallel mission to the better-known Qing survey of Chinese in Cuba, Yung Wing's trip to Peru has received almost no scholarly attention. Drawing on new archival evidence, this essay provides a historical analysis of the Qing mission to Peru and its official findings, as rendered in the original, 1874 English-language translation, “Yung Wing's Reports.” I argue that Yung Wing elaborated a multivocal, cross-cultural, and transimperial condemnation of Chinese indenture in Peru. Yung Wing served as a crucial broker across political and cultural systems. He strategically mobilized testimonies from American, Chilean, and Chinese informants and deployed abolitionist discourse to equate Chinese contract labor with African slavery. Denouncing Western failures to guarantee liberal principles for Chinese people, he pressured Qing authorities to protect Chinese subjects by opening formal legations in Latin America. Chinese workers in Peru had a different perspective, and their testimonies often diverged from Yung Wing's core argument. Chinese informants never equated contract labor with chattel slavery. Instead, they denounced employer failure to fulfill existing contract terms. Moreover, they provided evidence of successful collective efforts and alliances between Chinese and non-Chinese people that pressured Peruvian authorities to defend Chinese workers. Taken as a whole, “Yung Wing Reports” both elaborates a powerful Chinese denunciation of contract labor and demonstrates Chinese ability to negotiate its terms.
Thromboembolic Events With the Woven Endobridge Device: Incidence, Predictive Factors, and Management
Authors: Rodriguez-Erazú Fernanda , Cortese Jonathan , Mihalea Cristian , Popica Adrian , Chalumeau Vanessa , Vasconcellos Natalia , Gallas Sophie , Ikka Leon , Grimaldi Lamiae , Caroff Jildaz , Spelle Laurent 
Published: September 2023

BACKGROUND and OBJECTIVES:
The Woven EndoBridge (WEB) device has been increasingly used to treat wide-neck aneurysms showing a safe and effective profile, but a relatively high number of thromboembolic events (TEEs) have been reported with such treatment. We aimed to evaluate the incidence and management of TEEs and possible predictive factors related to WEB embolization of ruptured and unruptured intracranial aneurysms.

METHODS:
A single-center database with consecutive aneurysms treated with a WEB device between July 2012 and May 2022 was reviewed for intraoperative and delayed TEEs. Univariate and multivariable analyses were used to determine factors associated with TEEs.

RESULTS:
A total of 266 independent aneurysms were treated with WEB devices in 245 patients (mean age 55.78 ± 11.64 years, 169 (63.5%) females, 80 (30%) ruptured). The overall rate of TEEs is 13% (35/266), including 8.7% intraoperative. Symptomatic TEEs with clinical sequelae at a 3-month follow-up are reported to be 2.6% (7/266) with no TEE-related mortality. Both the replacement of a WEB device during the procedure (adjusted odds ratio = 2.61, 95% CI 1.24-5.49; P = .01) and ruptured aneurysms (adjusted odds ratio = 2.74, 95% CI 1.31-5.7; P = .007) were independent predictors of TEEs. A case-by-case management of intraprocedural TEE is also presented; tirofiban was successfully used in most cases of this cohort.

CONCLUSION:
In this study, we demonstrated that ruptured aneurysms and WEB device replacement during the procedure were independent predictive factors for TEEs. As a result, making the correct choice of WEB is crucial for improving treatment outcomes. Moreover, with proper medical management of TEEs, minimal morbidity and no mortality could be achieved, which reinforces the safety of the technique.
Development and validation of sonological classification and scoring system for uterine adenomyosis: A pilot study
Authors: Sinha Sudwita , Agarwal Mukta , Bhadani Punam Prasad , Roy Ria , Sinha Upasna 
Published: September 2023

Background: Adenomyosis is a common disorder in women of reproductive age. The gold standard for diagnosis is histopathological examination of hysterectomy specimen. However, only a small percentage of women undergo surgery as treatment is primarily hormonal. Non-invasive methods of diagnosis include transvaginal sonography and magnetic resonance imaging. Patient management in adenomyosis is often based on ultrasonographic diagnosis alone, highlighting the importance of a uniform, reproducible, clinically relevant and validated sonological classification and scoring system. Although a few investigators have proposed classification and scoring system for diagnosis of adenomyosis, none of those have been validated yet. This study aimed to propose and validate a new sonological classification and scoring system for adenomyosis. Methods: This was a prospective observational pilot study. A new sonological classification and scoring system of adenomyosis was proposed based on topography, type, size and extent, which was validated by comparing the sonological reporting with histopathological reporting. The main outcome measures that were measured were rate of agreement (Cohen’s kappa) between the findings of sonologist and pathologist; and diagnostic accuracy of the sonological classification of adenomyosis. Results: This pilot study included 30 women who underwent hysterectomy over a time period of one year with ultrasonographic diagnosis of adenomyosis. The rate of agreement (Cohen’s kappa) between the findings of sonologist and pathologist showed substantial agreement (0.703) for topography and almost perfect agreement for type (0.896), extent (0.892) and size (0.898). Conclusions: Our newly proposed sonological classification and scoring system for adenomyosis is valid and can be used for clinical application in interpersonal communication between clinicians, to prognosticate patients about the disease severity, to assess the candidates for surgical management and in further studies to correlate with symptoms severity and effectiveness of medical therapies.
Integrated Hand Weeding and Herbicide Application for Corn Marigold (Glebionis segetum) in Tef Fields across Western and Southwestern Shewa Zones in Ethiopia
Authors: Hundessa Niguse , Dechassa Nagassa , Bedada Shashitu , Bekele Getachew , Duguma Mosisa 
Published: September 2023

Background: Corn marigold (Glebionis segetum (L.) Frourr.) is a serious weed species of agricultural crops in many parts of Ethiopia. However, there is a lack of information on its management in tef fields in the country. Therefore, the current work was designed with the objectives of evaluating integrated management of the weed and determining the most economical integrated weed management options in tef fields. Methods: The field experiments were conducted in naturally corn marigold infested fields of Cheliya and Woliso districts of West and Southwest Shewa Zones in Ethiopia, respectively, during 2020 to 2021 cropping seasons. Results: Different corn marigold management practices exhibited different responses to different parameters of the target weed and the test crop differently. Application of Pyroxsulam 45g L-1 at a rate of 0.40 l ha -1 supplemented with once-hand weeding and Halauxifen-methyl 100g kg-1 + Florasulam 100g kg-1 sprayed at a rate of 50 g ha -1 supplemented with once-hand weeding increased grain yield by about 70 and 50%, respectively, over the weedy check. The marginal revenue and benefit-cost ratio obtained from the marketing of goods for the application of Pyroxsulam 45g L-1 with one supplementary hand weeding of corn marigold increased by110 89%, respectively, as compared to the weedy check. Conclusions: The present study revealed the role of integrated management of corn marigold in improving tef production and productivity by combating the weed. Future research should be directed towards scaling up and popularizing these technologies among the growers.