bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicities
Authors: Raghavan Abinaya , Kashyap Rachana , Sreedevi P , Jos Sneha , Chatterjee Suchana , Alex Ann , D’Souza Michelle Ninochka , Giridharan Mridhula , Muddashetty Ravi , Manjithaya Ravi , Padavattan Sivaraman , Nath Sangeeta 
Published: August 2023

AbstractAstrocytic cells are a subtype of glial cells that engulf pathogenic aggregates derived from degenerative neurons to facilitate its degradation. Here, we show that exposure to α-SYN protofibrils caused a transient increase in biogenesis of tunneling nanotubes (TNTs) in primary astrocytes and astrocyte-origin cancer cell-lines (U-87 MG, U251). Biogenesis of nascent TNTs corresponds to α-SYN protofibril-induced organelle toxicities, increased reactive oxygen species (ROS), and oxidative stress-induced premature cellular senescence. These TNTs mediate cell-to-cell transfer of α-SYN protofibrils, toxic lysosomes and mitochondria. Biogenesis of TNTs precedes clearance of α-SYN-induced organelle toxicities, cellular ROS levels and reversal of cellular senescence. Consequences of cellular clearance results in enhanced cell proliferation. Further, we have shown α-SYN-induced senescence promotes transient localization of focal adhesion kinase (FAK) in the nucleus. FAK mediated regulation of Rho-associated kinases may have a role in the biogenesis of TNTs, successively proliferation. Our study emphasizes that TNT biogenesis may have a potential role in the clearance of α-SYN toxicities and reversal of stress-induced cellular senescence, consequences of which cause enhanced proliferation in the post-recovered astroglia cells.Highlightsα-SYN protofibrils treated astroglia cells proliferate upon transient biogenesis of TNTs.Transient TNT biogenesis precedes clearance of α-SYN toxicities and reversal of senescence.Stress-induced senescence results in nuclear localization of FAK and ROCK mediated TNT biogenesis.The rescued cells enhance proliferation through ROCK mediated ERK1/2 and NFκB signalling cascades.SynopsisGraphical Abstract:α-SYN protofibrils-induced biogenesis of tunneling nanotubes (TNTs) aids to enhance cellular clearance of toxic burdens as a cellular survival strategy. α-SYN protofibrils treated toxic senescence cells regulate FAK mediated modulation of ROCK signalling cascades to promote TNT biogenesis and rescue the cellular toxicities. The rescued cells eventually enhance cell proliferation.
Integrated high-confidence and high-throughput approaches for quantifying synapse engulfment by oligodendrocyte precursor cells
Authors: Kahng Jessica A. , Xavier Andre M. , Ferro Austin , Auguste Yohan S.S. , Cheadle Lucas 
Published: August 2023

AbstractOligodendrocyte precursor cells (OPCs) sculpt neural circuits through the phagocytic engulfment of synapses during development and in adulthood. However, precise techniques for analyzing synapse engulfment by OPCs are limited. Here, we describe a two-pronged cell biological approach for quantifying synapse engulfment by OPCs which merges low-and high-throughput methodologies. In the first method, an adeno-associated virus encoding a pH-sensitive, fluorescently-tagged synaptic marker is expressed in neuronsin vivo.This construct allows for the differential labeling of presynaptic inputs that are contained outside of and within acidic phagolysosomal compartments. When followed by immunostaining for markers of OPCs and synapses in lightly fixed tissue, this approach enables the quantification of synapses engulfed by around 30-50 OPCs within a given experiment. In the second method, OPCs isolated from dissociated brain tissue are fixed, incubated with fluorescent antibodies against presynaptic proteins, and then analyzed by flow cytometry. This approach enables the quantification of presynaptic material within tens of thousands of OPCs in less than one week. These methods extend beyond the current imaging-based engulfment assays designed to quantify synaptic phagocytosis by brain-resident immune cells, microglia. Through the integration of these methods, the engulfment of synapses by OPCs can be rigorously quantified at both the individual and populational levels. With minor modifications, these approaches can be adapted to study synaptic phagocytosis by numerous glial cell types in the brain.
NMR measurements of transient low-populated tautomeric and anionic Watson-Crick-like G·T/U in RNA:DNA hybrids: Implications for the fidelity of transcription and CRISPR/Cas9 gene editing
Authors: Szekely Or , Rangadurai Atul Kaushik , Gu Stephanie , Manghrani Akanksha , Guseva Serafima , Al-Hashimi Hashim M. 
Published: August 2023

ABSTRACTMany biochemical processes use the Watson-Crick geometry to distinguish correct from incorrect base pairing. However, on rare occasions, mismatches such as G•T/U can transiently adopt Watson-Crick-like conformations through tautomerization or ionization of the bases, giving rise to replicative and translational errors. The propensities to form Watson-Crick-like mismatches in RNA:DNA hybrids remain unknown, making it unclear whether they can also contribute to errors during processes such as transcription and CRISPR/Cas editing. Here, using NMRR1ρexperiments, we show that dG•rU and dT•rG mismatches in two RNA:DNA hybrids transiently form tautomeric (Genol•T/U ⇄G•Tenol/Uenol) and anionic (G•T−/U−) Watson-Crick-like conformations. The tautomerization dynamics were like those measured in A-RNA and B-DNA duplexes. However, anionic dG•rU−formed with a ten-fold higher propensity relative to dT−•rG and dG•dT−and this could be attributed to the lowerpKa(ΔpKa∼0.4-0.9) of U versus T. Our findings suggest plausible roles for Watson-Crick-like G•T/U mismatches in transcriptional errors and CRISPR/Cas9 off-target gene editing, uncover a crucial difference between the chemical dynamics of G•U versus G•T, and indicate that anionic Watson-Crick-like G•U−could play a significant role evading Watson-Crick fidelity checkpoints in RNA:DNA hybrids and RNA duplexes.
A chromosome-level genome assembly ofZasmidium syzygiiisolated from banana leaves
Authors: van Westerhoven A.C. , Mehrabi R. , Talebi R. , Steentjes M.B.F. , Corcolon B. , Chong Pablo A. , Kema G.H.J. , Seidl M. F. 
Published: August 2023

AbstractAccurate taxonomic classification of samples from infected host material is essential for disease diagnostics and genome analyses. Despite the importance, diagnosis of fungal pathogens causing banana leaf diseases remains challenging. Foliar diseases of bananas are mainly caused by threePseudocercosporaspecies, of which the most predominant causal agent isP. fijiensis. Here, we sequenced and assembled four fungal isolates obtained from necrotic banana leaves in Bohol (Philippines) and obtained a high-quality genome assembly for one of these isolates. The samples were initially identified asP. fijiensisusing PCR diagnostics, however, the assembly size was consistently 30 Mb smaller than expected. Based on the ITS sequences, we identified the samples asZasmidium syzygii(98.7% identity). The high-qualityZasmidium syzygiiassembly is 42.5 Mb in size, comprising 16 contigs, of which 11 are complete. The genome contains 98.6% of the expected single-copy BUSCO genes and contains 14,789 genes and 10.3% repeats. The three short-read assemblies are less continuous but have similar genome sizes (40.4 – 42.4 Mb) and contain between 96.5% and 98.4% BUSCO genes. All four isolates have identical ITS sequences and are distinct fromZasmidiumisolates that were previously sampled from banana leaves. We thus report the first continuous genome assembly of a member of theZasmidiumgenus, forming an essential resource for further analysis to enhance our understanding of the diversity of pathogenic fungal isolates as well as fungal diversity.
Aromatic natural products synthesis from aromatic lignin monomers usingAcinetobacter baylyiADP1
Authors: Biggs Bradley W. , Tyo Keith E. J. 
Published: August 2023

AbstractAchieving sustainable chemical synthesis and a circular economy will require process innovation to minimize or recover existing waste streams. Valorization of lignin biomass has the ability to advance this goal. While lignin has proved a recalcitrant feedstock for upgrading, biological approaches can leverage native microbial metabolism to simplify complex and heterogeneous feedstocks to tractable starting points for biochemical upgrading. Recently, we demonstrated that one microbe with lignin relevant metabolism,Acinetobacter baylyiADP1, is both highly engineerable and capable of undergoing rapid design-build-test-learn cycles, making it an ideal candidate for these applications. Here, we utilize these genetic traits and ADP1’s native β-ketoadipate metabolism to convert mock alkali pretreated liquor lignin (APL) to two valuable natural products, vanillin-glucoside and resveratrol. En route, we create strains with up to 22 genetic modifications, including up to 8 heterologously expressed enzymes. Our approach takes advantage of preexisting aromatic species in APL (vanillate, ferulate, andp-coumarate) to create shortened biochemical routes to end products. Together, this work demonstrates ADP1’s potential as a platform for upgrading lignin waste streams and highlights the potential for biosynthetic methods to maximize the existing chemical potential of lignin aromatic monomers.
Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload
Authors: Copeland Caroline E. , Heitmeier Chloe J. , Doan Khoa D. , Lee Shea C. , Kwon Yong-Chan 
Published: August 2023

AbstractThe cell-free system offers potential advantages in biosensor applications, but their limited substrate supply poses a challenge in balancing enough sensing resources to detect low limits of analyte while providing a robust output signal. In this study, we harnessed split versions of fluorescent proteins, particularly split super-folder green fluorescent protein and mNeonGreen, to improve energy efficiency and enhance detection limit in the cell-free system. A comparative analysis of the expression of 1-10 and eleventh segments of beta strands in both whole-cell and cell-free platforms revealed distinct fluorescence patterns. Moreover, integrating SynZip peptide linkers substantially improved complementation, achieving a fluorescence intensity reaching 73.6% of the full-length protein and a 4.8-fold increase in expression compared to the split system without the SynZip peptide linkers. The split protein reporter system can enable energy-efficient sensing of low analyte levels in the cell-free system, broadening the toolbox of cell-free biosensor repertoire.
Decomposition of value added in gross exports: a critical review
Authors: Feás Enrique 
Published: August 2023

PurposeThe purpose of this paper is to settle the methodological debate on the decomposition of value added in gross exports, proposing a standard, exposing the drawbacks of the alternatives and quantifying the differences.Design/methodology/approachThis paper systematizes the analytical framework and assesses and quantifies the various methodologies and its main differences.FindingsThe decomposition method of Borin and Mancini (2023), using a source-based approach and an exporting country perspective, should be considered as the standard for decomposing the value added in gross exports. This study finds that alternative approaches and perspectives are methodologically inferior, and that tailored perspectives do not provide an increase in accuracy that compensates their drawbacks.Originality/valueThis paper’s contribution is fourfold: it rejects the alleged equivalence between approaches and perspectives, defending the superiority of a particular method, approach and perspective; it gives quantitative examples of the differences between them; it proves that the drawbacks of tailored perspectives do not compensate their alleged accuracy (as they do not result in big quantitative differences with the standard perspective); and it argues that no valid standard decomposition can forego the calculation of value added exported, which requires the expression of exports in terms of final demand.
Framework for cloud product lifecycle management system: a case study of an automotive industry
Authors: Tanpure Ganesh , Yadav Vinod , Jain Rakesh , Soni Gunjan 
Published: August 2023

PurposeProduct Lifecycle Management (PLM) systems have gained wide popularity for their role in manufacturing organizations for creating, managing and distributing product data. These systems are one of various enterprise systems which are required for smooth functioning and meeting the scaling up requirements organization. However, with introduction of cloud technology and other industry 4.0 initiatives, there has been focus on moving the on-premises IT application to the cloud. Such a move needs to be carried out by identifying and evaluating various challenges. This paper aims to discuss the aforementioned objective.Design/methodology/approachThe challenges identified through literature review have also been confirmed to be present via interview, system observation and documentation review through case study-based validation in an automotive component manufacturing industry.FindingsThe article identifies needs and challenges of cloud PLM systems and presents a generic framework for developing an approach for cloud PLM journey for an organization. The article also provides an approach for resolving the different challenges to realizing the designed system.Originality/valueThe simplified generic framework has been presented for use by industry professionals and researchers for designing cloud PLM systems that would fulfill expectations of different levels of stakeholders.
Mentoring at work: a talent development tool for Gen Y and Gen Z
Authors: Saxena Anubhuti 
Published: August 2023

Purpose
The aim of the article is to explore how mentoring can act as a strategy for developing talent of the emerging young workforce, which predominantly comprises Gen Y and Gen Z.

Design/methodology/approach
This briefing is authored by an independent writer who provides insightful commentary and contextualizes the articles.

Findings
To gain a competitive edge, firms must adapt to modern ways of Talent Development that foster a passion for work among new hires. Modern ways of mentoring such as Reverse Mentoring, Group Mentoring, Distance Mentoring or Anonymous Mentoring, Flash Mentoring or Speed Mentoring, Peer Mentoring can enable employers to be proactive in managing talent and foster better intergenerational relationships at the workplace.

Practical implications
As Gen Y and Gen Z are redefining workplace norms, it is crucial for HR partitioners to find out new ways to align the entire HR function with the needs of these tech-savvy generations and ensure they can perform to their potential. This study sheds light on various Mentoring methods, which managers can use to transform conventional approaches to Talent Development.

Originality/value
The briefing streamlines the reading process for busy executives and researchers by curating the most relevant information and presenting it in a concise and user-friendly format, allowing them to save time and quickly absorb key insights.
Talent trouble at a start-up: the case of TechShlok
Authors: Sharma Pooja , Bhattacharya Sanjay , Jain Avi 
Published: August 2023

Learning outcomes
This case study aims to understand the criticalities of hiring in a knowledge-based start-up; the various ways of retaining talent in a knowledge-based start-up; the competency requirements of human resource professionals in start-up organizations; the trade-off between business profitability and the R&D drive of the company; and the various organizational structures available for a knowledge-based start-up.

Case overview/synopsis
Ayush Jain, a first-generation entrepreneur, is facing a challenge with his start-up, TechShlok. TechShlok is an IT solution provider with its headquarters in Jaipur, India. The company is doing very well and is able to achieve many accolades. As the company grows in business and employee strength, it is facing talent acquisition and retention challenges. Ayush is in a fix to solve the talent issue and retain the key talent of the organization.

Complexity academic level
The case can be taught in a Human Resource Management course, including “talent management” and “human resource planning”. The case may be taught at upper undergraduate levels. It can also be taught in courses of entrepreneurship and knowledge-based start-ups.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 11: Strategy.
The impact of cyberattacks awareness on customers’ trust and commitment: an empirical evidence from the Pakistani banking sector
Authors: Bajwa Ishtiaq Ahmad , Ahmad Shabir , Mahmud Maqsood , Bajwa Farooq Ahmad 
Published: August 2023

PurposeThe banking industry has always been vulnerable to cyberattacks. In recent years, Pakistan’s banking sector experienced the most intense cyberattack in its over 70-year history. Due to these attacks, a large number of debit card accounts of major banks were negotiated. This study aims to examine the impact of cyberattack awareness and customers’ commitment levels after these cyberattacks.Design/methodology/approachThe study integrated the commitment–trust theory framework for the relationship of trust and commitment to the usage of online banking services. The partial least square structural equation modeling is being used to explore the relationship between customer’s trust, which is an outcome of continuous usage, and customer perception of affirmative cybersecurity measures the bank.FindingsThe findings revealed that customer trust in online banking is positively associated with customer commitment, but customers’ cyberattack awareness negatively impacts customer trust and commitment to online banking.Practical implicationsThe study highlights the importance of proactive communication, transparency and robust incident response that helps organizations establish themselves as trustworthy entities while prioritizing customer information and transaction protection.Originality/valueThe authors report on how cyberattacks on the banking sector influence the trust and commitment of the customers in the sector. The variable of cyberattack awareness used in this study is novel in online banking literature.
Into the Shark tank: an exploratory analysis of pharmapreneurial motivation and intention in Jordan
Authors: Ayesh Osama Mohammad , Zeqiri Jusuf , Gleason Kimberley , Alserhan Baker Ahmad 
Published: August 2023

Purpose
This paper aims to investigate the relationship between entrepreneurial motivation and pharmapreneurial intention among pharmacists in Jordan.The authors also assessed the moderating effect of gender and the COVID-19 pandemic on the relationship between these two variables.

Design/methodology/approach
This study is a quantitative study targeting pharmacists and using a nonprobability sample. A structured questionnaire was distributed through official channels to the entire population of registered pharmacists in Jordan. SmartPLS 3 was used for carrying an advanced structural equation model analysis, and SPSS 26 for the descriptive statistics.

Findings
The three entrepreneurial motivation variables (behavioral control [BC], risk tolerance and desirability of self-employment) affected pharmapreneurial intention, with BC contributing more to pharmapreneurial intention among pharmacists in Jordan than the other variables examined. On the other hand, gender and COVID-19 did not moderate this relationship.

Research limitations/implications
The authors find that entrepreneurial motivation affects pharmapreneurial intention. Consequently, the authors provide insights to policymakers and educators regarding dovetailing the recent financial literacy programs implemented in Jordanian schools with medical education in Jordan to better motivate the formation of pharmapreneurial intention.

Practical implications
Policymakers and educators should be dovetailing the recent financial literacy programs implemented in Jordanian schools with medical education in Jordan to better motivate the formation of pharmapreneurial intention.

Originality/value
To the best of the authors’ knowledge, this is the first study to examine pharmapreneurship in Jordan.
Co-branding drivers between professional sport organizations and on-field sports apparel sponsors
Authors: Abdolmaleki Hossein , Mohammadi Sardar , Babaei Mehdi , Soheili Behzad , Dickson Geoff , Funk Dan 
Published: August 2023

PurposeThis study aims to investigate drivers of co-branding, and the relative strength of these drivers within the Persian Gulf Pro League (PGPL). The study examines sport sponsorship, and specifically the relationship between professional football teams and on-field apparel sponsors.Design/methodology/approachSixteen experts participated in semi-structured interviews and ranked the co-branding drivers. The expert opinions were organized into estimates and triangular fuzzy numbers were established before the Mamdani Fuzzy Inference System converted the fuzzy outputs into crisp output values using the Centroid method. Next, the rankings of the drivers by the same 16 participants were analyzed using the analytical hierarchy process (AHP).FindingsThe study identified four main factors with 19 indicators: brand management (i.e. enhancing brand value, utilizing the knowledge and experience of partner brands, brand position, brand identity, brand equity and brand image), partner relationships (i.e. satisfaction, mutual trust, commitment, common interest, product reliability and innovative strategies), marketing factors (i.e. marketing mix, market position, competitive advantage and entry into new domestic markets) and supporting factors (i.e. copyright, contracts and social media law). The AHP identified the most influential factors as marketing, partner relationships, brand management and support.Originality/valueBased on the study’s findings, the authors recommend that PGPL teams adopt a partnership mindset, seek alignment of values and recognize the plurality of stakeholders to a sponsorship and their relationships to each other. The study highlights the challenges of co-branding activities in a developing country where trademark laws are not well developed.
Artificial intelligence (AI) for user experience (UX) design: a systematic literature review and future research agenda
Authors: Stige Åsne , Zamani Efpraxia D. , Mikalef Patrick , Zhu Yuzhen 
Published: August 2023

PurposeThe aim of this article is to map the use of AI in the user experience (UX) design process. Disrupting the UX process by introducing novel digital tools such as artificial intelligence (AI) has the potential to improve efficiency and accuracy, while creating more innovative and creative solutions. Thus, understanding how AI can be leveraged for UX has important research and practical implications.Design/methodology/approachThis article builds on a systematic literature review approach and aims to understand how AI is used in UX design today, as well as uncover some prominent themes for future research. Through a process of selection and filtering, 46 research articles are analysed, with findings synthesized based on a user-centred design and development process.FindingsThe authors’ analysis shows how AI is leveraged in the UX design process at different key areas. Namely, these include understanding the context of use, uncovering user requirements, aiding solution design, and evaluating design, and for assisting development of solutions. The authors also highlight the ways in which AI is changing the UX design process through illustrative examples.Originality/valueWhile there is increased interest in the use of AI in organizations, there is still limited work on how AI can be introduced into processes that depend heavily on human creativity and input. Thus, the authors show the ways in which AI can enhance such activities and assume tasks that have been typically performed by humans.