bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain‐wide mapping of efferent projections of glutamatergic (Onecut3+) neurons in the lateral mouse hypothalamus
Authors: Zupančič Maja , Tretiakov Evgenii , Máté Zoltán , Erdélyi Ferenc , Szabó Gábor , Clotman Frédéric , Hökfelt Tomas , Harkany Tibor , Keimpema Erik 
Published: April 2023

AbstractAimThis study mapped the spatiotemporal positions and connectivity of Onecut3+ neuronal populations in the developing and adult mouse brain.MethodsWe generated fluorescent reporter mice to chart Onecut3+ neurons for brain‐wide analysis. Moreover, we crossed Onecut3‐iCre and Mapt‐mGFP (Tau‐mGFP) mice to visualize axonal projections. A dual Cre/Flp‐dependent AAV construct in Onecut3‐iCre cross‐bred with Slc17a6‐FLPo mice was used in an intersectional strategy to map the connectivity of glutamatergic lateral hypothalamic neurons in the adult mouse.ResultsWe first found that Onecut3 marks a hitherto undescribed Slc17a6+/Vglut2+ neuronal cohort in the lateral hypothalamus, with the majority expressing thyrotropin‐releasing hormone. In the adult, Onecut3+/Vglut2+ neurons of the lateral hypothalamus had both intra‐ and extrahypothalamic efferents, particularly to the septal complex and habenula, where they targeted other cohorts of Onecut3+ neurons and additionally to the neocortex and hippocampus. This arrangement suggests that intrinsic reinforcement loops could exist for Onecut3+ neurons to coordinate their activity along the brain's midline axis.ConclusionWe present both a toolbox to manipulate novel subtypes of hypothalamic neurons and an anatomical arrangement by which extrahypothalamic targets can be simultaneously entrained.
Marriage equality & intersectionality
Authors: Robinson Russell K. , Frost David M. 
Published: April 2023

AbstractThe goal of this study is to understand the extent to which a diverse group of sexual and gender minorities understood the landmark Supreme Court ruling in favor of marriage equality as personally impacting them. Prominent lesbian, gay, bisexual, transgender, and queer (LGBTQ) figures have argued that marriage is an oppressive institution and that legalizing same‐sex marriage would not benefit the most marginalized members of the community, particularly Black people. Until now, there have been few resources for comparing these claims of scholars and activists with those of members of the communities they claim to represent. Guided by Critical Race Theory and intersectionality, this study centered LGBTQ people of color's lived experiences. A purposive sample of 99 LGBTQ people in Chicago, New York City, and San Francisco were asked whether and how the Supreme Court's decision in Obergefell v. Hodges (2015) personally impacted them. Most participants described an emotional impact. Relatively few criticized marriages as “heteronormative” or unfit for LGBTQ people. Black participants were less likely than participants of other races to criticize marriage as an institution. Moreover, Black and Latinx participants articulated a more expansive, equality‐focused understanding of the right to marry than the Court itself articulated. They described the marriage decision as carrying the potential to empower and elevate their identities in various contexts. For these people, the marriage equality movement was centrally about equality rather than marriage.
Adoption des Politiques de Protection de l'Environnement et performance des Entreprises Camerounaises
Authors: André Dumas Tsambou , Benjamin Fomba Kamga 
Published: April 2023

AbstractThe objective of this paper is to evaluate the effect of the environmental protection policies of by Cameroonian firms on their performance. It uses the endogenous switching regression technique and propensity scores applied to micro‐data from 639 firms in Cameroon. The results show that only 17% of firms adopt these measures, while on average 85% of firms produce solid, gaseous or liquid waste. The results also indicate that the adoption of these environmental protection policies increases operating costs while significantly improving the turnover and the performance of the productive capacity of the company. These increases are 39.11%, 58.6%, and 38.63% for operating costs, turnover and return on productive capacity of the company, respectively. However, firms can also suffer significant losses resulting from the non‐adoption of environmental policies. In fact, firms that do not adopt environmental protection policies have their performance reduced by an average of 1.625 percentage points.
Management of small (T1–T2) anal margin squamous cell carcinoma: clinical outcomes following local excision alone
Authors: Roji A. M. , Namiq K. F. , Radley S. , Ismail T. , Hejmadi R. , Taniere P. , Geh J. I. 
Published: April 2023

AbstractAimSquamous cell carcinomas of the anus are normally treated with synchronous chemoradiotherapy (CRT). Small, localized anal margin tumours may be adequately treated by local excision (LE) alone. This study aims to investigate the outcomes of patients with anal margin tumours treated with LE alone, reserving the use of CRT for salvage on local recurrence (LR).MethodsPatients with small, localized (stage I/IIA) anal margin tumours treated by LE from October 1999 to September 2018 were identified. The effect of tumour size and resection margin on LR risk was analysed. Outcomes of overall survival and disease‐free survival were measured.ResultsFifty‐five patients with anal margin tumours were identified. Overall 5‐year LR, overall survival and disease‐free survival rates were 8%, 86% and 82% respectively. Of the seven LRs, five were successfully salvaged with CRT with no further recurrence and two were not fit for CRT. Resection margins in non‐fragmented tumours and tumour size did not significantly influence LR risk.ConclusionsMost small, localized anal margin tumours can be adequately treated by LE alone with low LR rates. Most patients who developed LR were salvaged using CRT, with no cancer‐related deaths reported.
Laparoscopic ovarian transposition prior to pelvic radiation in young women with anorectal malignancies: a systematic review and meta‐analysis of prevalence
Authors: Tessier Léa , McKechnie Tyler , Lee Yung , Park Lily J. , Gangam Nirupa , Eskicioglu Cagla 
Published: April 2023

AbstractAimYoung women undergoing radiotherapy (RT) for pelvic malignancies are at risk of developing premature ovarian insufficiency. Ovarian transposition (OT) aims to preserve ovarian function in these patients. However, its role in anorectal malignancy has yet to be firmly established. The aim of this review was to determine the effectiveness of laparoscopic OT in preserving ovarian function in premenopausal women undergoing neoadjuvant pelvic RT for anorectal malignancies.MethodsMEDLINE, Embase and CENTRAL were systematically searched from inception through to May 2022. Articles were included if they evaluated ovarian function after OT in women with anorectal malignancies undergoing pelvic RT. The primary outcome was ovarian function preservation. The secondary outcome was 30‐day postoperative morbidity following OT.ResultsFrom 207 citations, 10 studies with 133 patients with rectal or anal cancer who underwent OT prior to RT were included. Meta‐analysis of pooled proportions of preserved ovarian function demonstrated an incidence of 66.9% (95% CI 55.0–79.0%, I2 = 43%). The 30‐day postoperative morbidity rate was 1.2% (n = 1). There was heterogeneity in interventions and outcome reporting.ConclusionsLaparoscopic OT in premenopausal patients undergoing pelvic radiation for anorectal malignancies might be an effective technique at reducing ovarian exposure to RT. The meta‐analyses must be interpreted within the context of clinical heterogeneity of the included studies. Further studies are required to fully investigate the outcomes of OT in patients undergoing pelvic radiation for anorectal malignancies.
How important are minimum wage increases in increasing the wages of minimum wage workers?
Authors: Clemens Jeffrey , Strain Michael R. 
Published: April 2023

AbstractPopular discussion presumes minimum wage increases primarily drive wage gains for minimum wage workers. We investigate this presumption using the Current Population Survey to assess the fraction of minimum wage workers receiving raises after 12 months. This fraction is moderately higher following state minimum wage increases, and positively correlated with several measures of labor market tightness. Finally, wage gains frequently follow industry and/or occupation switches, highlighting the importance of career progression for earnings growth among entry‐level workers. Career progression and increases in labor demand rather than minimum wage increases appear to drive most wage gains for minimum wage workers.
How Exergaming with Virtual Reality Enhances Specific Cognitive and Visuo‐Motor Abilities: An Explorative Study
Authors: Grosprêtre Sidney , Marcel‐Millet Philémon , Eon Pauline , Wollesen Bettina 
Published: April 2023

AbstractVirtual reality (VR) is the computer simulation of a three‐dimensional environment that a person can interact with using special electronic equipment, such as a headset with an integrated display. Often coupled with VR, exergames are video games that involve physical exercise. Little is known regarding the chronic effects of exergaming through VR chon cognitive functions. Eleven young participants were enrolled in this crossover exploratory study. They had to follow two trainings of 5 consecutive days, 15 min per day, interspaced by a 1‐month washout period. Trainings were performed in a random order: (1) a video training using shadow boxing fitness videos (SBV) and (2) a VR training using a three‐dimensional game where the aim is to cut moving cubes with a sword in each hand. Before and after each training period, a battery of cognitive tests was performed to assess executive functions, such as attention (change blindness), reaction time, response inhibition (go/no‐go, Stroop task), or flexibility (trail making test). Fine motor skills were also evaluated through a Fitt's task. No effect of the SBV training was observed on any of the cognitive functions tested. On the contrary, a significant increased performance in selective attention and observation tests was found after VR training, as well as in inhibitory processes (Stroop and go/no‐go). Other performances were unaffected by either VR or SBV training. The present study argues that VR exergaming is a promising tool to promote cognitive enhancement but targets specific functions according to the type of interface/game that is used.
A systematic review on the impact of commercially available hybrid closed loop systems on psychological outcomes in youths with type 1 diabetes and their parents
Authors: Franceschi Roberto , Mozzillo Enza , Di Candia Francesca , Maines Evelina , Leonardi Letizia , Girardi Martina , Fedi Ludovica , Rosanio Francesco Maria , Marcovecchio M. Loredana 
Published: April 2023

AbstractAimTo systematically assess the impact of commercially available hybrid closed loop (HCL) systems on psychological outcomes in youths with type 1 diabetes and their parents.MethodsWe performed a systematic review including studies published in the last 10 years. PICOS framework was used in the selection process, and evidence was assessed using the GRADE system.ResultsA total of 215 studies were identified after duplicate removal, and 31 studies were included in this systematic review: 20 on first‐generation HCL and 11 on second‐generation HCL systems. According to studies with moderate‐ to high‐level quality of evidence, HCL systems led to better, or in some studies, unchanged psychological outcomes such as distress and burden related to diabetes management, fear of hypoglycemia, quality of life, satisfaction; instead, quality of sleep was perceived as improved, although results were not confirmed in studies using actigraphy. From semi‐structured interviews, answers were more homogeneous, and participants reported a positive experience and attitude towards HCL technology, which was felt to be easy to use and apt to achieve glycemic targets.ConclusionsEvidence confirms the importance of evaluating the psychosocial needs of youths with diabetes and their families when starting HCL systems and during follow‐up, and to set realistic expectations of what can be achieved along with awareness of the limitations of the systems, and educate and motivate families to overcome barriers.
Prognostic role of CRP‐independent inflammatory patterns in patients undergoing primary percutaneous interventions
Authors: Dossi Filippo , Raccis Mario , Parodi Guido , De Caterina Alberto Ranieri , Paradossi Umberto , Esposito Augusto , D'Agostino Andreina , De Luca Giuseppe , Berti Sergio 
Published: April 2023

AbstractBackgroundDespite the key pathophysiological role of inflammation in the development of coronary artery disease (CAD), the evaluation of inflammatory status has not been clearly established in patients presenting with acute coronary syndrome (ACS). The aim of this study is to evaluate the prevalence of CRP‐independent inflammatory patterns in patients referred for primary percutaneous coronary intervention (pPCI) and to determine their one‐year relationship with adverse clinical outcomes.MethodsWe carried out a single‐centre, observational study consecutively enrolling all patients presenting at a large‐volume PCI hub with a diagnosis of ST‐segment elevation myocardial infarction (STEMI) and treated with pPCI. Systemic immune‐inflammatory index (SII) was calculated at admission and discharge. According to different SII trajectories patients were divided into four patterns: ‘persistent‐low’, ‘down‐sloping’, ‘up‐sloping’ and ‘persistent‐high’ patterns. The primary endpoint was a composite of all‐cause of death and myocardial infarction (MI) at a one‐year follow‐up.ResultsAmong the total 2353 subjects enrolled, 44% of them belonged to ‘persistent‐low’, 31% to ‘down‐sloping’, 4% to ‘up‐sloping’ and 21% to ‘persistent‐high’ pattern. The primary endpoint was observed in 8% of patients with a ‘persistent‐low’, 12% with a ‘down‐sloping’, 27% with an ‘up‐sloping’ and 25% with a persistent‐high pattern (p = 0.001). After multivariate analysis, ‘up‐sloping’ (OR: 3.2 [1.59–3.93]; p = 0.001) and ‘persistent‐high’ (OR: 4.1 [3.03–4.65]; p = 0.001) patterns emerged as independent predictors of one‐year adverse events.Conclusions‘Persistent‐high’ and ‘up‐sloping’ CRP‐independent inflammatory patterns in patients undergoing primary PCI are associated with an increased risk of adverse events at one‐year follow‐up. The prognostic value of these inflammatory patterns might be helpful to individualize potential therapeutic targets.
Association between sleep traits and primary liver cancer: A Mendelian randomization analysis
Authors: Yang Xin , Wang Jingyi , Wang Huaqing 
Published: April 2023

AbstractBackgroundPrimary liver cancer (PLC) is the sixth most frequently occurring cancer, representing one of the top 5 leading causes of cancer‐related mortality worldwide. Recently, researchers have focused more on the impact of living habits on the incidence and development of tumours. This study reports a relationship between sleep traits and PLC.MethodsIn this study, we used published genome‐wide association studies to obtain exposure factors of 6 sleep traits. Mendelian randomization (MR) analysis was used to assess the causal relationship between sleep traits and PLC via inverse‐variance weighted (IVW), MR Egger and weighted median. Sensitivity analysis was used to reduce the bias.ResultsOur investigation revealed that there was a negative correlation between sleep duration and the group of liver and bile duct cancer by IVW (p‐value = .042), and this result was similarly observed in the liver cell carcinoma group by Weighted Median (p‐value = .026). In contrast, there was a positive correlation found between napping during the day and primary liver cancer in the cohorts of liver and bile duct cancer (p‐value = .030), liver cell carcinoma (p‐value = .043) and malignant neoplasm of other and unspecified parts of the biliary tract (p‐value = .016) by IVW. Furthermore, our study also revealed a positive correlation between insomnia and malignant neoplasm of the liver and intrahepatic bile ducts by IVW (p‐value = .022).ConclusionsOverall, our study indicates that insomnia and nap during the day may be risk factors of PLC and adequate night sleep might keep us away from PLC.
Effects of MBOAT7 polymorphism and steatosis on liver function assessed by methacetin breath test
Authors: Di Ciaula Agostino , Bonfrate Leonilde , Shanmugam Harshitha , Weber Susanne N. , Krawczyk Marcin , Portincasa Piero 
Published: April 2023

AbstractBackgroundMBOAT7 rs641738 variant is a risk factor for nonalcoholic fatty liver disease (NAFLD) and liver fibrosis, but the relationship between this polymorphism and early liver dysfunction remains uncertain.MethodsEighty outpatients underwent blood analyses, liver imaging by ultrasound and acoustic radiation force impulse shear wave elastography and were genotyped for MBOAT7 (wild‐type [WT], rs641738 heterozygous or homozygous) polymorphism using TaqMan assays.ResultsNAFLD was confirmed in 53 patients. Portal uptake and hepatocyte microsomal metabolization of (13C)‐methacetin were explored by measuring 13CO2 appearance in exhaled air. The distribution of the MBOAT7 genotypes was comparable in subjects with or without NAFLD. The majority of subjects with or without NAFLD had fibrosis ≤ F1 but decreased portal extraction of (13C)‐methacetin, i.e. 78.6% in homozygous, 45.0% in heterozygous and 46.2% in WT for the MBOAT7 variant. Both substrate extraction and microsomal metabolization were mostly defective in the homozygous carriers. The extraction efficiency from portal blood flow was minimal in subjects with both homozygous rs641738 polymorphism and NAFLD, as compared to those with WT/heterozygous polymorphism, with or without NAFLD.ConclusionsThe homozygous MBOAT7 rs641738 polymorphism per se is associated with a reduced extraction efficiency of (13C)‐methacetin from the portal flow pointing to subclinical liver dysfunction independently from liver fibrosis. Liver steatosis worsens (13C)‐methacetin extraction efficiency. We urge to better explore the mechanisms of interaction between external factors and multiple gene polymorphisms (including MBOAT7), paving the road to primary prevention and novel therapeutic strategies.
Aggregate and individual effects of information in a coordination (traffic) game
Authors: Ashraf Sruthi , Brown Alexander L. , Burris Mark W. , Vitaku Valon 
Published: April 2023

AbstractUsing an existing coordination (traffic) experiment, we investigate information's effect on traffic congestion when subjects already have a history of past play. In contrast to previous studies, our interventions neither alter aggregate nor individual payoffs. A second study isolates individual‐subject response to information using a fixed distribution of past subjects. We find information alters subject play: subjects switch roads more often and receive higher payoffs conditional on switching roads. Because switching reduces payoffs unconditionally, information does not generally improve payoffs overall. Only subjects that receive information upon starting the game appear to increase their payoffs due to the information treatment.
Serum neurofilament light chain cut‐off definition for clinical diagnosis and prognosis of amyotrophic lateral sclerosis
Authors: Brousse Mehdi , Delaby Constance , De La Cruz Elisa , Kuhle Jens , Benkert Pascal , Mondesert Etienne , Ginestet Nelly , Hirtz Christophe , Camu William , Lehmann Sylvain , Esselin Florence 
Published: April 2023

AbstractBackgroundThe neurofilament light chain (NfL) assay is gradually becoming an essential diagnostic tool for the diagnosis of many neurological diseases including amyotrophic lateral sclerosis (ALS). Different methods for the determination of this biomarker in serum have been developed in recent years.MethodsWe measured blood NfL in 429 patients referred to the tertiary ALS center of Montpellier, France using two different ultrasensitive methods (Ella™ and Simoa™) and we compared the clinical performances of these two approaches. We also converted NfL values into age and body mass index‐adjusted Z‐scores to assess cut‐off values of this biomarker in this clinical context.ResultsWe show comparable diagnostic and prognostic performance of Ella™ and Simoa™ technologies in ALS, with specificities and sensitivities exceeding 80% for both. We propose cut‐off values for serum NfL in this clinical context, thus enabling the routine clinical use of this biomarker.ConclusionThe use of NfL in routine clinical practice will help predict survival and improve diagnostic accuracy by distinguishing ALS from other neurological diseases and motor neuron disease mimics.
Actigraphic correlates of neuropsychiatric symptoms in adults with focal epilepsy
Authors: Abboud Mark A. , Kamen Jessica L. , Bass John S. , Lin Lu , Gavvala Jay R. , Rao Sindhu , Smagula Stephen F. , Krishnan Vaishnav 
Published: April 2023

AbstractObjectivesDisability in patients with epilepsy (PWEs) is multifactorial: beyond seizure frequency/severity, PWEs are prone to a range of neuropsychiatric, cognitive, and somatic comorbidities that significantly affect quality of life. Here, we explored how variations in seizure severity and the burden of self‐reported somatic/neuropsychiatric symptoms correlate with disruptions to 24 h activity patterns (rest‐activity rhythms [RARs]), determined through wrist accelerometry/actigraphy.MethodsMultiday wrist‐actigraphy recordings were obtained from 59 adult patients with focal epilepsy (44% male, ages 18–72), who contemporaneously responded to validated psychometric instruments to measure anxiety, depression, sleepiness, and somatic symptoms. We conducted a similar in silico psychometric‐actigraphic correlation in a publicly available data set of 1747 Hispanic subjects (35% male, ages 18–65) from the Study of Latinos (SOL) Sueño Ancillary Study. RARs were analyzed via a sigmoidally‐transformed cosine model (quantifying amplitude, steepness, acrophase, and robustness) and nonparametric measures to estimate RAR stability, fragmentation, and sleep.ResultsCompared with matched SOL subjects, RARs from PWE subjects featured a significantly lower amplitude, a wider rest phase, and significantly more total daily sleep. Within PWEs, similar RAR distortions were associated with seizure intractability and/or anticonvulsant polytherapy, whereas high anxiety, depression, and somatic symptom scores were associated with lower RAR robustness and acrophase delay. We applied the SOL data set to train logistic regression models to dichotomously classify subjective anxiety, depression, and sleepiness symptoms using demographic and RAR parameters. When tested on PWEs, these models predicted prevalent anxiety and depression symptom burden (accuracy ~70%) but failed to predict subjective sleepiness.SignificanceTogether these results demonstrate that RAR features may encode prevalent depression and anxiety symptoms in patients with focal epilepsy, potentially offering wearable‐derived endpoints to adjunct clinical care and drug/device trials. With larger PWE‐specific actigraphic‐psychometric data sets, we may identify RAR signatures that may more precisely correlate with varying seizure frequency, the burden of anticonvulsant therapy, and prevalent mood/anxiety symptoms.