bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating collaborative scenarios for short food supply chains: a case study on high‐level processing technology
Authors: Van Parys Esther , Tran Duc , Sampers Imca , Benezech Thierry , Loveniers Pieter‐Jan , Devlieghere Frank , De Steur Hans , Gellynck Xavier , Schouteten Joachim J. 
Published: June 2023

SummaryThis study examined three collaborative scenarios for the implementation of a high‐level aseptic filling machine in small‐scale food processing for improved food safety in short food supply chains. A two‐stage research design was implemented, based on an exploration and evaluation phase. Two workshops were organised to conceptualise the following scenarios: mobile packaging, cooperative and individual ownership. A novel approach to the strengths, weaknesses, opportunities and threats‐analytical hierarchy process methodology was used to compare stakeholder perceptions and preferences for different scenarios within one case study. Financial benefits played a key role in each collaborative scenario. The most suitable scenario was found to be the mobile packaging, offering flexibility and no initial investment cost while enabling collaborations. The cooperative scenario has the potential for centralised logistics and marketing while being conscious of its organisational structure. Individual ownership should also focus on centralised logistics and marketing while considering food safety and flexibility limitations.
Usage of online food delivery in food waste generation in China during the crisis of COVID‐19
Authors: Wei Liuchun , Prabhakar Guru , Duong Linh N. K. 
Published: June 2023

SummaryThis study connects food waste (FW) with online food delivery (OFD) service and aims to explore the impacts of the usage of OFD service on FW generation in China during the COVID‐19 lockdown period. Through responses from 123 consumers of OFD service in China, results show that the usage of OFD service did not accelerate FW generation in China during the COVID‐19 lockdown period. We found that consumers tended to order excessive food when using OFD services for some economic reasons before the pandemic, which was a significant driver behind FW generation in the OFD segment. However, most consumers admitted that they tended to stockpile food at the beginning of the lockdown period. Because of the impacts of COVID‐19 on consumer appetite and portion size, there was an overall decrease in FW generation during the COVID‐19 lockdown period in China. Therefore, it is suggested that restaurants provide adjusted portions of ordered food based on the scientific investigation or provide more pre‐packaged food for a long shelf‐life. Consequently, these measures will lead to less FW generation in the OFD service segment in China.
First identification and histopathological analysis of Lactococcus garvieae infection in whiteleg shrimp, Penaeus vannamei cultured in low salinity water
Authors: Ballantyne Rolissa , Lee Jai‐Wei , Liu Chun‐Hung 
Published: June 2023

AbstractThe isolation and characterization of bacterial species Lactococcus garvieae, previously unreported in whiteleg shrimp, Penaeus vannamei, has now been identified in the species. The pathogen was recovered from an affected shrimp farm in southern Taiwan. Bacterial characterization first identified the isolate as Gram‐positive cocci, and biochemical profiles demonstrated that the causative agent of mortality was 97% L. garvieae. The bacterial cell DNA resulted in amplification of 1522 bp with 99.6% confirmation by PCR analysis. The phylogenetic tree revealed 100% evolutionary similarity among previously isolated strains. Experimental infection further confirmed higher susceptibility of whiteleg shrimp to L. garvieae in waters of lower salinity, particularly 5 ppt, than in higher salinity. Histopathological analysis showed severely damaged hepatopancreas with necrotized, elongated, collapsed tubules, dislodged membranes and granuloma formation in infected shrimp. Transmission electron microscopy observation indicated a hyaluronic acid capsular layer surrounding bacterial cell which is a virulence factor of L. garvieae and likely responsible for immunosuppression and higher mortality of shrimp cultured in lower salinity. Collectively, these findings report the first isolation of L. garvieae from whiteleg shrimp and shed new light on the disease that threatens the highly valuable species and accentuates the need for finding a solution.
In vitro phenotypic evidence for the utilization of iron from different sources and siderophores production in the fish pathogen Tenacibaculum dicentrarchi
Authors: Avendaño‐Herrera Ruben , Saldarriaga‐Córdoba Mónica , Echeverría‐Bugueño Macarena , Irgang Rute 
Published: June 2023

AbstractIron uptake during infection is an essential pathogenicity factor of several bacteria, including Tenacibaculum dicentrarchi, an emerging pathogen for salmonid and red conger eel (Genypterus chilensis) farms in Chile. Iron‐related protein families were recently found in eight T. dicentrarchi genomes, but biological studies have not yet confirmed functions. The investigation reported herein clearly demonstrated for the first time that T. dicentrarchi possesses different systems for iron acquisition—one involving the synthesis of siderophores and another allowing for the utilization of heme groups. Using 38 isolates of T. dicentrarchi and the type strain CECT 7612T, all strains grew in the presence of the chelating agent 2.2′‐dipyridyl (from 50 to 150 μM) and produced siderophores on chrome azurol S plates. Furthermore, 37 of the 38 T. dicentrarchi isolates used at least four of the five iron sources (i.e. ammonium iron citrate, ferrous sulfate, iron chloride hexahydrate, haemoglobin and/or hemin) when added to iron‐deficient media, although the cell yield was less when using hemin. Twelve isolates grew in the presence of hemin, and 10 of them used only 100 μM. Under iron‐supplemented or iron‐restricted conditions, whole cells of three isolates and the type strain showed at least one membrane protein induced in iron‐limiting conditions (c.a. 37.9 kDa), regardless of the isolation host. All phenotypic results were confirmed by in‐silico genomic T. dicentrarchi analysis. Future studies will aim to establish a relationship between iron uptake ability and virulence in T. dicentrarchi through in vivo assays.
Nodular gill disease in Northeastern Italy: An investigation on the prevalence of the disease and the risks of introduction in rainbow trout farms
Authors: Cocco Alessia , Toson Marica , Perolo Alberto , Casarotto Claudia , Franzago Eleonora , Brocca Ginevra , Verin Ranieri , Quaglio Francesco , Dalla Pozza Manuela , Bille Laura 
Published: June 2023

AbstractNodular Gill Disease (NGD) is an emerging pathogenic condition that causes gill damage and mainly affects farmed freshwater fish, rainbow trout (Oncorhynchus mykiss) in particular, and this inevitably generates noticeable economic losses for the industry. The present study aimed to assess the prevalence of NGD in the Autonomous Province of Trento, a highly productive area located in Northeastern Italy, traditionally suited to rainbow trout production, and to identify possible risk factors for the introduction of this disease in trout farms. The necessary data were obtained through a questionnaire and the collection of fish samples. According to the data analysis, 42% of the investigated farms tested positive for NGD. The two possible risk factors identified for its introduction in farms are the presence of other diseases in the same farm (OR = 17.5; 95% CI = 2.7; 111.5) and having farms located 5 km upstream (OR = 24.8; 95% CI = 2.9; 211.1). These results highlight (i) a possible impairment of the immune system caused by other diseases as a predisposing factor to the manifestation of the pathology and (ii) the role of water in spreading pathogens.
METTL14 is decreased and regulates m6A modification of α‐synuclein in Parkinson's disease
Authors: He Honghu , Zhang Qinxin , Liao Jiajia , Lei Jiang , Luo Man , Huang Jianyi , Chen Meiling , Shen Yuefei , Wang Jin , Xu Pingyi , Xiao Yousheng 
Published: June 2023

AbstractN6‐methyladenosine (m6A), an emerging modification of messenger RNA, has been implicated in many biological processes. However, its role in Parkinson's disease (PD) remains largely unknown. Here, we investigated the role of m6A modification and its underlying mechanism in PD. First, 86 individuals with PD and 86 healthy controls were recruited from a pilot multicenter cohort. Levels of m6A and its modulators in peripheral blood mononuclear cells of patients with PD and controls were measured using an m6A RNA methylation quantification kit and quantitative real‐time PCR. The underlying mechanism of m6A modification in PD was investigated in vitro through RNA immunoprecipitation assay, RNA stability assay, gene silencing or overexpression, western blot, and confocal immunoassay. The results show that mRNA levels of m6A, METTL3, METTL14, and YTHDF2 in patients with PD were significantly lower than in healthy controls, and METTL14 was the main factor involved in abnormal m6A modification. Area under the curve (AUC) analysis suggests METTL14 may provide excellent diagnostic capability for PD, especially when combined with plasma α‐synuclein (α‐syn). Spearman correlation analysis identified that METTL14 was moderately negatively correlated with plasma α‐syn and the motor function of PD. Mechanistic experiments demonstrated that Mettl14 targets and regulates the expression of the α‐syn gene using its methylation function. Overexpression of Mettl14 dramatically increased m6A modification of α‐syn mRNA and weakened its stability. Further results suggest that α‐syn mRNA was modified by Mettl14 binding of an m6A motif in the coding region of α‐syn mRNA, while the reading protein Ythdf2 was involved in recognizing m6A‐modified α‐syn mRNA. Taken together, our results reveal the potential of METTL14 as a novel diagnostic biomarker for PD and identify modification of pathogenic α‐syn protein by METTL14 via an m6A‐YTHDF2‐dependent mechanism.image
Combinatorial drug‐loaded quality by design adapted transliposome gel formulation for dermal delivery: In vitro and dermatokinetic study
Authors: Qadir Abdul , Ullah Shehla Nasar Mir Najib , Gupta Dipak Kumar , Khan Nausheen , Warsi Musarrat Husain , Kamal Mehnaz 
Published: June 2023

AbstractBackgroundUrsolic acid is a powerful drug that possesses many therapeutic properties, such as hepatoprotection, immunomodulation, anti‐inflammatory, antidiabetic, antibacterial, antiviral, antiulcer, and anticancer activity. Centella asiatica (L.) Urban (Umbelliferae) contains a triterpene called asiatic acid, which has been used effectively in traditional Chinese and Indian medicine system for centuries. Anticancer, anti‐inflammatory, and neuroprotective properties are only some of the many pharmacological actions previously attributed to asiatic acid .AimThe present work developed an optimized combinatorial drug‐loaded nano‐formulation by Quality by design approach.Materials and MethodsThe optimize transliposome for accentuated dermal delivery of dual drug. The optimization of drug‐loaded transliposome was done using the “Box–Behnken design.” The optimized formulation was characterized for vesicles size, entrapment efficiency (%), and in vitro drug release. Additionally, transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), and dermatokinetic study were performed for further evaluation of drug‐loaded optimized transliposome formulation.ResultsThe optimized combinatorial drug‐loaded transliposome formulation showed a particle size of 86.36 ± 2.54 nm, polydispersity index (PDI) 0.230 ± 0.008, and an entrapment efficiency of 87.43 ± 2.66% which depicted good entrapment efficiency. In vitro drug release of ursolic acid and asiatic acid transliposomes was found to be 85.12 ± 2.54% and 80.23 ± 3.23%, respectively, as compared to optimized ursolic acid and asiatic acid transliposome gel drug release that was 67.18 ± 2.85% and 60.28 ± 4.12%, respectively. The skin permeation study of ursolic and asiatic acid conventional formulation was only 32.48 ± 2.42%, compared with optimized combinatorial drug‐loaded transliposome gel (79.83 ± 4.52%) at 12 h. After applying combinatorial drug‐loaded transliposome gel, rhodamine was able to more easily cross rat skin, as observed by confocal laser scanning microscopy, in comparison with when the rhodamine control solution was used.DiscussionThe UA_AA‐TL gel formulation absorbed more ursolic acid and asiatic acid than the UA_AA‐CF gel formulation, as per dermatokinetic study. Even after being incorporated into transliposome vesicles, the antioxidant effects of ursolic and asiatic acid were still detectable. In most cases, transliposomes vesicular systems generate depots in the skin's deeper layers and gradually release the medicine over time, allowing for fewer applications.ConclusionIn overall our studies, it may be concluded that developed dual drug‐loaded transliposomal formulation has great potential for effective topical drug delivery for skin cancer.
Fractional picosecond laser for atrophic acne scars: A meta‐analysis
Authors: Li Jing , Duan Fangfang , Kuang Jie 
Published: June 2023

AbstractBackgroundConventional fractional lasers (FLs) are well‐established treatments for acne scars with some inevitable adverse events. Fractional picosecond laser (FPL) is increasingly used for acne scars.AimsTo compare the efficacy and safety of FPL with non‐picosecond FLs for acne scars.MethodsPubMed, Embase, Ovid, Cochrane Library, and Web of Science databases were searched. We also searched ClinicalTrials, WHO ICTRP, and ISRCTN websites. A meta‐analysis was conducted to assess the clinical improvement and adverse events after FPL compared with other FLs.ResultsOverall, seven eligible studies were included. Three physician evaluation systems showed no difference between FPL and other FLs in clinical improvement of atrophic acne scars (MD = 0.64, 95% CI:−9.67 to 10.94; MD = −0.14, 95% CI:‐0.71 to 0.43; RR = 0.81, 95% CI:0.32 to 2.01). Patient‐assessed effectiveness was also not significantly different between FPL and other FLs (RR = 1.00, 95% CI:0.69 to 1.46). Although temporary pinpoint bleeding was more common after FPL (RR = 30.33, 95% CI:6.14 to 149.8), the incidence of post‐inflammatory hyperpigmentation (PIH) and pain level were lower for FPL (RR = 0.16, 95% CI:0.06 to 0.45; MD = −1.99, 95% CI:−3.36 to −0.62). Additionally, edema severity after treatment did not differ between the two groups (MD = −0.35, 95% CI:−0.72 to 0.02). As for the duration of erythema, no difference between FPL and nonablative FL groups (MD = −1.88, 95% CI:−6.28 to 2.51).ConclusionsFPL seems similar to other FLs regarding clinical improvement of atrophic acne scars. With lower PIH risk and pain scores, FPL is more suitable for acne scar patients prone to PIH or sensitive to pain.
The impact of safety culture, quality of care, missed care and nurse staffing on patient falls: A multisource association study
Authors: Alanazi Faisal Khalaf , Lapkin Samuel , Molloy Luke , Sim Jenny 
Published: June 2023

AbstractAimsTo examine the association between nursing unit safety culture, quality of care, missed care and nurse staffing levels, and inpatient falls using two data sources: incidence of falls and nurses' perceptions of fall frequency in their units. The study explores the association between the two sources of patient falls and identifies if nurses' perceptions of patient fall frequency reflect the actual patient falls recorded in the incident management system.BackgroundInpatient falls are associated with severe complications that result in extended hospitalisation and increased financial consequences for patients and healthcare services.DesignA multi‐source cross‐sectional study guided by the STROBE guidelines.MethodsA purposive sample of 33 nursing units (619 nurses) from five hospitals completed an online survey from August to November 2021. The survey measured safety culture, quality of care, missed care, nurse staffing levels and nurses' perceptions of patient fall frequency. In addition, secondary data on falls from participating units between 2018 and 2021 were also collected. Generalised linear models were fitted to examine the association between study variables.ResultsNursing units with strong safety climate and working conditions and lower missed care were associated with lower rates of falls using both data sources. Nurses' perceptions of the frequency of falls in their units were reflective of the actual incidence rate of falls, but the association was not statistically significant.ConclusionNursing units with a strong safety climate and better collaborations between nurses and other professionals, including physicians and pharmacists, were associated with lower incidents of patient falls.Relevance to Clinical PracticeThis study provided evidence for healthcare services and hospital managers to minimise patient falls.Patient or Public ContributionPatients who had experienced a fall, which was reported in the incident management system, from the included units in the five hospitals were part of this study.
The Ralstonia pseudosolanacearum effector RipE1 is recognized at the plasma membrane by NbPtr1, the Nicotiana benthamiana homologue of Pseudomonas tomato race 1
Authors: Kim Boyoung , Kim Injae , Yu Wenjia , Li Meng , Kim Haseong , Ahn Ye Jin , Sohn Kee Hoon , Macho Alberto P. , Segonzac Cécile 
Published: June 2023

AbstractThe bacterial wilt disease caused by soilborne bacteria of the Ralstonia solanacearum species complex (RSSC) threatens important crops worldwide. Only a few immune receptors conferring resistance to this devastating disease are known so far. Individual RSSC strains deliver around 70 different type III secretion system effectors into host cells to manipulate the plant physiology. RipE1 is an effector conserved across the RSSC and triggers immune responses in the model solanaceous plant Nicotiana benthamiana. Here, we used multiplexed virus‐induced gene silencing of the nucleotide‐binding and leucine‐rich repeat receptor family to identify the genetic basis of RipE1 recognition. Specific silencing of the N. benthamiana homologue of Solanum lycopersicoides Ptr1 (confers resistance to Pseudomonas syringae pv. tomato race 1) gene (NbPtr1) completely abolished RipE1‐induced hypersensitive response and immunity to Ralstonia pseudosolanacearum. The expression of the native NbPtr1 coding sequence was sufficient to restore RipE1 recognition in Nb‐ptr1 knockout plants. Interestingly, RipE1 association with the host cell plasma membrane was necessary for NbPtr1‐dependent recognition. Furthermore, NbPtr1‐dependent recognition of RipE1 natural variants is polymorphic, providing additional evidence for the indirect mode of activation of NbPtr1. Altogether, this work supports NbPtr1 relevance for resistance to bacterial wilt disease in Solanaceae.
Conventional and contemporary approaches for drought tolerance rice breeding: Progress and prospects
Authors: Anilkumar C , Sah Rameswar Prasad , Beena Radha , Azharudheen TP Muhammed , Kumar Awadesh , Behera Sasmita , Sunitha NC , Pradhan SK , Reshmi Raj KR , C Parameswaran , Marndi BC , Singh Anil Kumar 
Published: June 2023

AbstractDrought is becoming a major threat to rice farming across the globe owing to the depletion of water tables in rice‐growing belts. Drought affects rice plants at multiple stages, causing damage at morphological and physio‐biochemical levels, leading to severe losses that exceed losses from all other stresses. The amalgamation of conventional breeding methods with modern molecular biology tools and biometrical methods could help accelerate the genetic gain for drought tolerance in rice. Many drought‐tolerance traits with genetic determinants have been identified and exploited for tolerance rice variety breeding. The integration of genome‐wide association study and genomic selection tools with speed breeding shortened the breeding cycle and aided in rapid improvement of genetic gain. In this review, we emphasized the progress made through classical breeding as well as the limitations and usefulness of current genomic methods in improving drought tolerance. We briefly addressed methods for identifying genetic determinants for drought tolerance and deploying them through genomics‐assisted breeding programmes to develop high‐yielding drought‐tolerant rice cultivars.
Genetic control underlying the flowering‐drought tolerance trade‐off in the Antarctic plant Colobanthus quitensis
Authors: Galleguillos Carolina , Acuña‐Rodríguez Ian S. , Torres‐Díaz Cristian , Gundel Pedro E. , Molina‐Montenegro Marco A. 
Published: June 2023

AbstractPlants inhabiting environments with stressful conditions often exhibit a low number of flowers, which can be attributed to the energetic cost associated with reproduction. One of the most stressful environments for plants is the Antarctic continent, characterized by limited soil water availability and low temperatures. Induction of dehydrins like those from the COR gene family and auxin transcriptional response repressor genes (IAAs), which are involved in floral repression, has been described in response to water stress. Here, we investigated the relationship between the water deficit‐induced stress response and the number of flowers in Colobanthus quitensis plants collected from populations along a latitudinal gradient. The expression levels of COR47 and IAA12 genes in response to water deficit were found to be associated with the number of flowers. The relationship was observed both in the field and growth chambers. Watering the plants in the growth chambers alleviated the stress and stimualted flowering, thereby eliminating the trade‐off observed in the field. Our study provides a mechanistic understanding of the ecological constraints on plant reproduction along a water availability gradient. However, further experiments are needed to elucidate the primary role of water availability in regulating resource allocation to reproduction in plants inhibiting extreme environments.
Songwashing: Russian Popular Music, Distraction, and Putin’s Fourth Term
Authors: Biasioli Marco 
Published: June 2023

AbstractThis article investigates how the Russian state apparatus, in its diverse, loose, and undercoordinated points of affiliation, used contemporary popular music to its own advantage during Vladimir Putin’s fourth term and before the full‐scale war in Ukraine. I propose that the different state branches’ efforts at appropriating music in order to distract audiences can be grouped into a single, novel framework of “songwashing.” This entails the state utilization of the allure of music to cover up domestic political issues, such as problems with legitimation, civic discontent, and minority rights, while promoting a different, positive image abroad. Following two case studies—the 2019 summer festivals Shashlik Live and Meat & Beat in Moscow, and Tajik‐born singer Manizha’s participation in the 2021 Eurovision Song Contest—the article also assesses how songwashing mechanisms are exposed and resisted.