bluuebunny/crossref_metadata_2025_split huggingface dataset:
The oxoglutarate dehydrogenase complex is involved in myofibril growth and Z-disc assembly in Drosophila
Authors: González Morales Nicanor , Marescal Océane , Szikora Szilárd , Katzemich Anja , Correia-Mesquita Tuana , Bíró Péter , Erdelyi Miklos , Mihály József , Schöck Frieder 
Published: June 2023

ABSTRACT
Myofibrils are long intracellular cables specific to muscles, composed mainly of actin and myosin filaments. The actin and myosin filaments are organized into repeated units called sarcomeres, which form the myofibrils. Muscle contraction is achieved by the simultaneous shortening of sarcomeres, which requires all sarcomeres to be the same size. Muscles have a variety of ways to ensure sarcomere homogeneity. We have previously shown that the controlled oligomerization of Zasp proteins sets the diameter of the myofibril. Here, we looked for Zasp-binding proteins at the Z-disc to identify additional proteins coordinating myofibril growth and assembly. We found that the E1 subunit of the oxoglutarate dehydrogenase complex localizes to both the Z-disc and the mitochondria, and is recruited to the Z-disc by Zasp52. The three subunits of the oxoglutarate dehydrogenase complex are required for myofibril formation. Using super-resolution microscopy, we revealed the overall organization of the complex at the Z-disc. Metabolomics identified an amino acid imbalance affecting protein synthesis as a possible cause of myofibril defects, which is supported by OGDH-dependent localization of ribosomes at the Z-disc.
Quaking regulates circular RNA production in cardiomyocytes
Authors: Montañés-Agudo Pablo , van der Made Ingeborg , Aufiero Simona , Tijsen Anke J. , Pinto Yigal M. , Creemers Esther E. 
Published: June 2023

ABSTRACT
Circular RNAs (circRNAs) are a class of non-coding RNA molecules that are gaining increasing attention for their roles in various pathophysiological processes. The RNA-binding protein quaking (QKI) has been identified as a regulator of circRNA formation. In this study, we investigate the role of QKI in the formation of circRNAs in the heart by performing RNA-sequencing on Qki-knockout mice. Loss of QKI resulted in the differential expression of 17% of the circRNAs in adult mouse hearts. Interestingly, the majority of the QKI-regulated circRNAs (58%) were derived from genes undergoing QKI-dependent splicing, indicating a relationship between back-splicing and linear splicing. We compared these QKI-dependent circRNAs with those regulated by RBM20, another cardiac splicing factor essential for circRNA formation. We found that QKI and RBM20 regulate the formation of a distinct, but partially overlapping set of circRNAs in the heart. Strikingly, many shared circRNAs were derived from the Ttn gene, and they were regulated in an opposite manner. Our findings indicate that QKI not only regulates alternative splicing in the heart but also the formation of circRNAs.
Muscle function during cross-country skiing at different speed and incline conditions
Authors: Werkhausen Amelie , Lundervold Anders , Gløersen Øyvind 
Published: June 2023

ABSTRACT
The human musculoskeletal system is well adapted to use energy-efficient muscle–tendon mechanics during walking and running, but muscle behaviour during on-snow locomotion is unknown. Here, we examined muscle and muscle–tendon unit behaviour during diagonal-style cross-country roller skiing at three speed and incline conditions to examine whether skiers can exploit energy-saving mechanisms of the muscle–tendon unit. We assessed lower leg muscle and muscle–tendon unit mechanics and muscle activity in 13 high-level skiers during treadmill roller skiing using synchronised ultrasound, motion capture, electromyography and ski-binding force measurements. Participants skied using diagonal style at 2.5 and 3.5 m s−1 up 5 deg, and at 2.5 m s−1 up 10 deg. We found an uncoupling of muscle and joint behaviour during most parts of the propulsive kick phase in all conditions (P&lt;0.01). Gastrocnemius muscle fascicles actively shortened ∼0.9 cm during the kick phase, while the muscle–tendon unit went through a stretch–shortening cycle. Peak muscle–tendon unit shortening velocity was 5 times faster than fascicle velocity (37.5 versus 7.4 cm s−1, P&lt;0.01). Steeper incline skiing was achieved by greater muscle activity (24%, P=0.04) and slower fascicle shortening velocity (3.4 versus 4.5 cm s−1, P&lt;0.01). Faster speed was achieved by greater peak muscle activity (23%, P&lt;0.01) and no change in fascicle shortening velocity. Our data show that, during diagonal-style cross-county skiing, muscle behaviour is uncoupled from the joint movement, which enables beneficial contractile conditions and energy utilisation with different slopes and speeds. Active preloading at the end of the glide phase may facilitate these mechanisms.
An Easy Learning Approach to a Complex Surgical Technique: A Step-by-Step Site-Relapse Lateral Extended Endopelvic Resection (LEER)
Authors: Lago Víctor , Guijarro-Campillo Alberto Rafael , Vidal Blanca Segarra , Padilla-Iserte Pablo , Matute Luis , Álvarez José Antonio Pérez , Del Pozo Santiago Domingo 
Published: June 2023

AbstractLateral pelvic sidewall involvement by gynecological tumors has been considered traditionally an absolute contraindication to curative resection.1 Moreover, the involvement of the pelvic sidewall at the time of relapse in cervical cancer after primary or adjuvant pelvic radiation occurs in 8.3% of patients.2,3 Laterally extended endopelvic resection (LEER), based on the ontogenetic compartment theory, provides a potential surgical option for patients for whom palliative therapy is the only alternative.4 This complex and ultraradical, surgical technique allows a high rate of complete resection in more than 70% of patients with gynecological cancers and lateral pelvic sidewall involvement. An adequate selection of patients and a deep knowledge of pelvic anatomy are crucial to obtain acceptable morbimortality rates and improved overall survival in this population.5 To deconstruct this complex procedure, we show a detailed step-by-step technique to facilitate the easy learning curve of this surgical technique. We review the Höckel original technique with different site-relapse adapted steps. We provide a pedagogical high-quality video (Video 1) and anatomical outline drawings (Fig. 1) to understand lateral pelvic wall anatomy and standardize this surgical technique. Our purpose is to bring this knowledge to gynecologists and pelvic surgeons in which pelvic lateral approach may be useful beyond gynecological oncologic surgery (Table 1).
Clinical Significance of Intramural Metastasis as an Independent Prognostic Factor in Esophageal Squamous Cell Carcinoma
Authors: Ushimaru Yuki , Makino Tomoki , Tanaka Koji , Yamashita Kotaro , Saito Takuro , Yamamoto Kazuyoshi , Takahashi Tsuyoshi , Kurokawa Yukinori , Nakajima Kiyokazu , Morii Eiichi , Eguchi Hidetoshi , Doki Yuichiro 
Published: June 2023

Abstract
Background
Although intramural metastasis (IM) in esophageal cancer is considered a poor prognostic factor, there are only limited reports detailing its clinicopathologic characteristics and prognostic impact.

Patients and Methods
We retrospectively included patients with esophageal squamous cell carcinoma (ESCC) with esophagectomy at our institution between 2010 and 2016. We compared patients with intramural metastases (IMs) (IM group) versus those without IMs (non-IM group) to clarify the clinical significance of intramural metastasis in ESCC.

Results
A total of 23 (3.9%) out of all 597 patients were identified to have IM. The IMs were located on the cranial side in 13 (56.5%) and caudal side in 10 (43.5%) of the primary tumor, with two multiple cases. The IM group, compared with the non-IM group, was associated with higher percentage of cN-positive (91.3 versus 67.9%, P = 0.02), pN-positive (82.6 versus 55.9%, P = 0.04), and pM(lym)-positive (30.4 versus 12.5%, P = 0.02) cases. Five-year recurrence-free survival (RFS) was significantly worse in the IM group than the non-IM group (14.9 versus 55.0 %, P < 0.001). Multivariable analysis of recurrence-free survival identified pT (HR 1.74, 95% CI 1.36–2.23, P < 0.001), pN (HR 2.11, 95% CI 1.60–2.78, P < 0.001), histological classification (HR 1.68, 95% CI 1.21–2.35, P = 0.002), and pM(LYM) (HR 1.64, 95% CI 1.64–2.95, P < 0.001), along with presence of IM (HR 2.24, 95% CI 1.37–3.64, P < 0.001) to be independent prognostic factors. Lymphatic (65.2 versus 24.9%, P < 0.001) and hepatic (26.1 versus 6.8%, P = 0.005) recurrences were significantly more common in the IM group than in the non-IM group.

Conclusions
IM was shown to be associated with dismal survival after surgery. A treatment strategy emphasizing more intensive systemic control should be considered for patients with ESCC with IM.
Functional and Oncological Outcome of Patients with Distal Femoral Osteosarcoma Managed by Limb Salvage Using Modular Endoprosthesis
Authors: Sadek Wael Mohamed Safwat , Ebeid Walid Atef , Ghoneimy Ahmed El , Ebeid Emad , Senna Wessam Gamal Abou 
Published: June 2023

Abstract
Background
The aim of the study was to assess the functional and oncological outcomes of patients with distal femoral osteosarcoma managed by limb salvage using modular endoprosthesis as well as to assess related complications.

Patients and Methods
A total of 82 patients were included in our study. Functional outcome was assessed using MSTS score and knee range of motion. Oncological outcome was assessed regarding local recurrence, chest metastasis, and patient survivorship. Complications were classified according to Henderson et al.

Results
The mean MSTS score was 26.21 (87.4%) (range 8–30 points) with 70.7% of patients having more than 90° of flexion. The incidence of local recurrence was 3.7% (3 patients), while the incidence of chest metastasis was 14.6% (12 patients). Aseptic loosening (type 2 failure) was the commonest complication (19.5%), followed by infection (15.9%). The 5- and 10-year survivorships of the limb were 98.8%, while the 5- and 10-year survivorships of the prosthesis were 67.7% and 52.4%, respectively.

Conclusion
This study showed that patients with osteosarcoma distal femur who are treated by chemotherapy and limb salvage have an excellent long-term prognosis in terms of patient as well as limb survivorship. The use of modular endoprosthesis in these patients offer an acceptable function, with two-thirds of the patients retaining their prosthesis after 5 years and more than half retaining them after 10 years.
The performance of lipid profiles and ratios as a predictor of arterial stiffness measured by brachial-ankle pulse wave velocity in type 2 diabetic patients
Authors: Soelistijo Soebagijo Adi , Adiwinoto Robert Dwitama , Pranoto Agung , Ardiany Deasy 
Published: June 2023

Background: Early identification of arterial stiffness in Type 2 diabetes mellitus (T2DM) patients before the manifestation of atherosclerosis would be clinically beneficial. Our study aimed to explore the correlation of lipid profiles and ratios with arterial stiffness, and construct a predictive model for arterial stiffness in T2DM patients using those parameters. Methods: One hundred and eighty-four adult T2DM patients in the diabetes outpatient clinic at the Dr. Soetomo general academic hospital were enrolled in this cross-sectional study in 2015 and 2019. Sociodemographic, glycosylated hemoglobin (HbA1c), lipid profiles, and brachial-ankle pulse wave velocity (ba-PWV) data were collected from all subjects. The subjects were divided into a group with arterial stiffness (ba-PWV > 18 m/sec) and without arterial stiffness (ba-PWV ≤ 18 m/sec). A correlation test was used to evaluate the association, and receiver operator characteristics (ROC) curves analysis were used to determine the cut-off value, sensitivity, and specificity. The risk analysis model was calculated using bivariate logistic regression analysis. Results: The group with arterial stiffness had higher lipid profiles: total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and lipid ratios. A significant positive correlation was found between TC, TG, LDL-C, and all lipid ratios with ba-PWV. A negative correlation was found between HDL-C and ba-PWV. All lipid ratio parameters can be used as predictors of arterial stiffness, especially non-HDL-C with cut-off value: 150 mg/dL (sensitivity 96.8% and specificity 52.9%) and TG/HDL-C ratio with cut-off value: 4.51 (sensitivity 81.0% and specificity 74.2%). Elevated TG/HDL-C ratio and non-HDL-C displayed higher risk (OR: 12.293 and 16.312; p < 0.05) of having arterial stiffness compared to other lipid ratios. Conclusions: Lipid profiles and lipid ratios, especially TG/HDL-C ratio and non-HDL-C, are potential biochemical markers for arterial stiffness in T2DM patients.
Case Report: Spontaneous pneumothorax revealing multiple myeloma: a case report
Authors: Brahem Mouna , BenTekaya Ramy , Touil Imen , Braham Yosra , Jomaa Olfa , Bousoffara Leila , knani Jalel , Boudawara Nedia , Hachfi Haifa , Younes Mohamed 
Published: June 2023

Various causes like trauma, infection, pulmonary disease or neoplasm can lead to spontaneous pneumothorax. We report a rare case of a spontaneous pneumothorax as first manifestation of multiple myeloma. A 58-year-old patient presented suffering from dyspnea and right-sided chest pain, with no history of trauma. On examination, the patient had bilateral rib tenderness. The respiratory rate was 30 breaths/min and oxygen saturation was 88%. The chest physical exam revealed unequal breath sounds, an hyperresonance with percussion and decreased wall movement on the right side. The analysis of arterial blood gas revealed hypoxemia (arterial oxygen tension: 7.59 kPa) and hypercapnia (arterial carbon dioxide tension: 5.99 kPa). Laboratory data showed a raised C reactive protein level (133.8 mg/L), hyper-calcemia (serum calcium: 12.18 mg/dL) and a decreased plasma albumin level (31.9 g/L). Chest radiography and thoracic computed tomography revealed multiple ribs and sternum fractures leading to a partial pneumothorax on the right side. Subsequent workup for multiple myeloma showed elevated levels of immunoglobulin. Results of initial laboratory tests revealed an IgG gamma paraprotein, a urine protein electrophoresis of 1450 mg/24 hours and a β-2 microglobulin rate of 3.35. The diagnosis of multiple myeloma was confirmed with a bone marrow infiltration of 20% of atypical plasmatic cells. Cytogenetic investigations did not show any chromosomal abnormalities, especially the t (4,14) translocation. The patient was diagnosed with multiple myeloma stage IIIA according to Durie–Salmon classification. Appropriate treatment with oxygen therapy and systemic analgesic was started, associated with a cure of zoledronic acid in order to decrease the calcium level. The evolution was characterized by the complete resolution of the pneumothorax in 7 days and the normalization of the calcium level. The autologous stem cell transplant was the treatment of choice for this patient.
Study of anaemia in pregnancy and low birth weight in fluoride endemic area of Western Rajasthan: a cohort study
Authors: Bhardwaj Pankaj , Mantri Neha , Joshi Nitin Kumar , Suthar Praveen , Sharma Praveen , Purohit Purvi , Singh Pratibha , Shekhar Shashank , Khandelwal Shweta , Dandabathula Giribabu , Raghav Pankaja Ravi , Ramawat Nikky , Jain Sanjeev , Patil Manoj , Gaidhane Abhay , Quazi Syed Zahiruddin , Saxena Deepak 
Published: June 2023

Background: Fluoride is a noxious element known to destroy gastrointestinal mucosa, leading to erythrocytes' destruction and causing anaemia. The birth weight of newborn babies is a significant indicator of a child's vulnerability to the risk of childhood diseases and chances of existence. Methods: This prospective cohort study was planned to find linkages between fluorosis and the low-birth weight of newborn babies with anaemic mothers. Antenatal mothers until the 20th week of gestation were followed up till delivery in the Antenatal Clinic of a District Hospital in one of the known fluoride-endemic districts (Nagaur) and the other not-so-endemic district (Jodhpur) of Western Rajasthan. Results: Around 19% of the newborn in Jodhpur and around 22% in Nagaur had low birth weight. Mean fluoride values in water samples were measured to be 0.57 (range from 0.0 to 2.7 PPM) in Jodhpur and 0.7 (range from 0.0 to 3.4 PPM) in Nagaur. Conclusions: Thus, in fluoride endemic areas, other factors should be included besides iron and folic acid supplementation for improving anaemia in pregnant women. This calls for assessing the effectiveness of de-fluoridation activities along with the area's most common indigenous food practices.
Celiac Disease and Migraine Headaches: Current Knowledge and Future Directions
Authors: Wojciechowska Klara , Ślósarz Tymoteusz , Turek Monika , Piątkowska Karolina , Walęcik-Kot Weronika 
Published: June 2023

Background:
Migraine headaches and celiac disease are widespread problems affecting millions of patients worldwide. Celiac disease is an autoimmune disease in which the consumption of gluten leads to the development of an abnormal immune response in the intestines and the atrophy of intestinal villi. The most characteristic clinical symptoms of celiac disease are gastrointestinal symptoms. Nevertheless, manifestations of the nervous system, including migraines, are also sometimes noted. A gluten-free diet is the only effective therapeutic approach for patients with celiac disease. It reduces symptoms and halts the progression of the disease. Neurological symptoms, including migraines, are frequent extraintestinal manifestations. In this study we aim to elaborate on current knowledge concerning both coexisting of migraines and celiac disease as well as effect of gluten free diet on headaches in patients with celiac disease, gluten intolerance and healthy individuals
Results:
Headaches, especially migraines, are more common in patients suffering from celiac disease. Numerous studies indicate that a gluten-free diet may help in the treatment of headaches and is also an effective way to stop the progression of celiac disease.
Conclusion:
Migraine headaches are quite common in patients with celiac disease, and a gluten-free diet may help alleviate them. The findings suggest the need for increased awareness of the co-occurrence of these two conditions. A gluten-free diet is the only effective treatment for celiac disease and can stop progression of the disease. Further research is needed to determine the potential benefits of such a diet in patients with gluten intolerance or healthy individuals.
A Unified Analysis of a Class of Proximal Bundle Methods for Solving Hybrid Convex Composite Optimization Problems
Authors: Liang Jiaming , Monteiro Renato D. C. 
Published: June 2023

This paper presents a proximal bundle (PB) framework based on a generic bundle update scheme for solving the hybrid convex composite optimization (HCCO) problem and establishes a common iteration-complexity bound for any variant belonging to it. As a consequence, iteration-complexity bounds for three PB variants based on different bundle update schemes are obtained in the HCCO context for the first time and in a unified manner. Although two of the PB variants are universal (i.e., their implementations do not require parameters associated with the HCCO instance), the other newly (as far as the authors are aware) proposed one is not, but has the advantage that it generates simple—namely, one-cut—bundle models. The paper also presents a universal adaptive PB variant (which is not necessarily an instance of the framework) based on one-cut models and shows that its iteration-complexity is the same as the two aforementioned universal PB variants. Funding: Financial support from the Office of Naval Research [N00014-18-1-2077] and the Air Force Office of Scientific Research [Grant FA9550-22-1-0088] is gratefully acknowledged.
Genetic diversity and phylogenetic analysis of mayfly Caenis (Insecta: Ephemeroptera) using Cytochrome C Oxidase I (COI) and 12s rRNA genes from Thailand
Authors: PRAKRONGRAK NARUMON , BOONSOONG BOONSATIEN , MONTHATONG MONTHIRA 
Published: June 2023

Abstract. Prakrongrak N, Boonsoong B, Monthatong M. 2023. Genetic diversity and phylogenetic analysis of mayfly Caenis (Insecta: Ephemeroptera) using Cytochrome C Oxidase I (COI) and 12s rRNA genes from Thailand. Biodiversitas 24: 1989-1997. Mayflies in genus Caenisis one of the top fifth species richnessbelonged to Family Caenidae. The present study aimed to use the partial mitochondrial Cytochrome COxidase subunit I (COI) and 12s rRNA nucleotide sequences for molecular species identification, diversity and phylogenetic relationships of mayflies in genus Caenis Stephens, 1835 species from Thailand. From a total of 37 specimens, thirteen nymphs were morphologically identified into five species: Caenis nasuta Malzacher, C. Picea Kimmins, C. ulmeriana Malzacher, C. cornigera Kang and Yang and C. longiforcipata Malzacher. The other 24 samples were Caenis sp.1 to sp.5. Partial COI sequences of all samples were compared with BOLD and GenBank for species identification. However, the result showed only genus-level identification as Caenis with greater than 80% similarity. For species delimitation, interspecific genetic distance among these species ranged from 14.4 to 26.6% (COI) and from 7.36 to 22.4% (12s rRNA). Intraspecific divergence levels were 0.0% to 2.35%. ABGD analysis of both genes divided data into 10 groups corresponding to 10 morphospecies of Caenis. The phylogenetic relationships of Caenis, COI and 12s rRNA genes also classified each species to well supported clusters. In addition, we report C. cornigera from Thailand for the first time. The COI and 12s rRNA phylogenetic trees indicate a close relationship between C. cornigera and C. longiforcipata, which is supported by their similar morphology.
Ant preference for different types of bait at sugarcane plantations in East Java, Indonesia
Authors: RAHARDJO BAMBANG TRI , MUHAMMAD FAIZ NASHIRUDDIN , SETIAWAN YOGO , FEBRYADI AKSHA , IHSAN MUHAMMAD , WIBOWO DENNIS , FERNANDO ITO 
Published: June 2023

Abstract. Rahardjo BT, Muhammad FN, Setiawan Y, Febryadi A, Ihsan M, Wibowo D, Fernando I. 2023. Ant preference for different types of bait at sugarcane plantations in East Java, Indonesia. Biodiversitas 24:2099-2106. The occurrence of ants is important in agroecosystems since they may provide both ecosystem services or disservices depending on the species composition. Therefore, it is necessary to investigate the kind of ant species that are present in agroecosystems. This study aimed to assess the effectiveness of three types of bait to measure ant abundance and diversity in sugarcane plantations. This research was conducted from July to September 2022 in four locations in East Java, Indonesia, i.e., Karangploso, Kalipare, Gandusari, and Pasirian. At each location, one plot was determined and divided into nine subplots. Ant sampling was conducted using modified pitfall traps that may contain one type of bait or without bait as control treatment. There were three types of assayed bait, i.e., tuna, eggs, and sugar. Nine traps for each treatment were installed in each location. This study obtained 6,650 individual ants from 20 morphospecies, 16 genera, and 4 subfamilies. At each location, species richness and abundance were not significantly different among bait types. However, those variables differed between the bait groups and the control. Tuna and egg baits were preferred by ants, as indicated by the higher number of trapped individuals than in sugar bait and the control traps. The species composition of the different baits was different at all locations except Kalipare. Each ant species had a different preference for the type of bait. Formicine ants preferred sugar bait, while ponerine ants preferred tuna and egg baits. Hence, it can be concluded that the bait type selection needs to be considered for ant monitoring. Therefore, using various types of baits is recommended to monitor the assemblage of ants in an ecosystem.