bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCSK9 involves in the high-fat diet-induced abnormal testicular function of male mice
Authors: Cui Zhi-hui , Ma Yong-dan , Wang Yi-cheng , Liu Huan , Song Jia-wei , Zhang Li-xue , Guo Wen-jing , Zhang Xue-qin , Tu Sha-sha , Yuan Dong-zhi , Zhang Jin-hu , Nie Li , Yue Li-min 
Published: February 2023

In brief
Impaired spermatogenesis resulting from disturbed cholesterol metabolism due to intake of high-fat diet (HFD) has been widely recognized, however, the role of preprotein invertase subtilin 9 (PCSK9), which is a negative regulator of cholesterol metabolism, has never been reported. This study aims to reveal the role of PCSK9 on spermatogenesis induced by HFD in mice.

Abstract
Long-term consumption of a high-fat diet (HFD) is an important factor that leads to impaired spermatogenesis exhibiting poor sperm quantity and quality. However, the mechanism of this is yet to be elucidated. Disrupted cholesterol homeostasis is one of many crucial pathological factors which could contribute to impaired spermatogenesis. As a negative regulator of cholesterol metabolism, preprotein invertase subtilin 9 (PCSK9) mediates low density lipoprotein receptor (LDLR) degradation to the lysosome, thereby reducing the expression of LDLR on the cell membrane and increasing serum low-density lipoprotein cholesterol level, resulting in lipid metabolism disorders. Here, we aim to study whether PCSK9 is a pathological factor for impaired spermatogenesis induced by HFD and the underlying mechanism. To meet the purpose of our study, we utilized wild-type C57BL/6 male mice and PCSK9 knockout mice with same background as experimental subjects and alirocumab, a PCSK9 inhibitor, was used for treatment. Results indicated that HFD induced higher PCSK9 expression in serum, liver, and testes, and serum PCSK9 is negatively correlated with spermatogenesis, while both PCSK9 inhibitor treatment and PCSK9 knockout methodologies ameliorated impaired lipid metabolism and spermatogenesis in mice fed a HFD. This could be due to the overexpression of PCSK9 induced by HFD leading to dyslipidemia, resulting in testicular lipotoxicity, thus activating the Bcl-2–Bax–Caspase3 apoptosis signaling pathway in testes, particularly in Leydig cells. Our study demonstrates that PCSK9 is an important pathological factor in the dysfunction of spermatogenesis in mice induced by HFD. This finding could provide innovative ideas for the diagnosis and treatment of male infertility.
A study on enhancement of red meat production and consumption estimation methods in Türkiye
Authors: Özbek F.Ş. 
Published: February 2023

AbstractIt is necessary to estimate the number of animals slaughtered outside slaughterhouses in countries like Türkiye, where slaughterings in non-slaughterhouses are relatively high. This paper presents the estimation of red meat production including all slaughterings both in slaughterhouses and in non-slaughterhouses by using a re-established model based on change in the inventory of animals including imported and exported animals, slaughtering rate for the slaughtered animals raised domestically in Türkiye, and total red meat consumption per capita per year in Türkiye. Total red meat production in Türkiye was estimated as 1 million 952 thousand 38 tonnes, bovine meat production was estimated as 1 million 471 thousand 550 tonnes, sheep and goats meat production was estimated as 480 thousand 488 tonnes in 2021. Total red meat human consumption per capita in Türkiye was estimated as 23 kg carcass weight per capita per year and 17 kg retail weight per capita per year. 17 kg of total red meat human consumption came from bovine meat, and 6 kg came from sheep and goats meat. According to the result of cluster analysis of European countries, Türkiye was clustered in the group of lowest total red meat consumption with Albania, Ukraine, Bosnia and Herzegovina, Republic of Moldova, North Macedonia, Czech Republic, and Serbia.
Preferences and knowledge of farmers and internet-orientated population about renewable energy sources in Kosovo
Authors: Pestisha Albiona , Bai Attila 
Published: February 2023

AbstractOur results introduce the preferences and attitudes of farmers and the internet-orientated population regarding renewable energy sources (RES) in Kosovo. The research included 243 internet-orientated participants and 30 farmers. The data was gathered through questionnaires (in both groups) and deep interviews (just in farmers). The results showed that the awareness of RES was high for especially solar, wind and hydro energy, the relationship among them was very strong. It was justified that firewood was typically used by respondents with low income, but not considered as renewable energy source, similarly to Hungary and the USA. A special feature of Kosovo is that electricity has very high importance for the man in the street, even for heating. Better education, higher income and environmental-consciousness definitely support better knowledge of RES, similarly to international tendencies. Males and employed participants had more knowledge about RES than females, or unemployed ones. Moreover, the results showed that the participants prefer environment rather than convenience, which is also typical of less wealthy countries. Our results might be useful as a typical less developed country case study for international comparison and helps to eliminate the unknown willingness in RES for the development of future agricultural strategies in Kosovo.
Socio-Demographic Construct of Social Loneliness in Modern Russia
Authors: Ildarhanova Chulpan I. , Gnevasheva Vera A. 
Published: February 2023

The article examines the theory and methodology behind social loneliness in the family context, as well as empirically assessing the socio-demographic manifestation of this phenomenon in modern Russia. Several factors exacerbating and minimizing social loneliness are presented. A strong emphasis is placed on the importance of families in alleviating social loneliness and on family construct preservation. Using the cluster method, the authors analyze criteria-defined groups from the general sample of an empirical study that was held by the Family and Demography Center of Tatarstan Academy of Sciences (TAS) in 2022. As a result, a conclusion is drawn about factors that the respondents consider to be the most significant in minimizing social loneliness. In this connection, the authors find it important to define the family as a synergistic mechanism for reducing such social risks and assess the risks of transforming the traditional family construct. In addition, the study uses the female subsample to examine the role of gender in the severity of social loneliness. Determining the role of women in the creation of family relations and the transfer of social value-based attitudes, the authors assess the family construct stability and the likely features of its transformation to develop a preventive socio-demographic policy for reducing social deviation and maintaining stable social value-based relationships.
Cardiomyocyte mitochondrial respiration and microviscosity in rats of different ages in heart failure
Authors: Korepanov V. A. , Rebrova T. Yu. , Gorbunov A. S. , Afanasiev S. A. 
Published: February 2023

Highlights. Heart failure in older age groups is an urgent medical and social issue. Mitochondrial dysfunction is a key link in the pathogenesis of heart failure. In this study we have demonstrated a decrease in mitochondrial respiratory function in old rats. In this age group, the development of heart failure is accompanied by a further decrease in respiratory control. An increase in the mitochondrial microviscosity in older animals may affect the activity of respiratory chain enzymes in heart failure.Aim. To study the respiratory activity and mitochondrial membrane microviscosity of cardiomyocyte of rats of different ages with heart failure.Methods. The study involved 22 2- and 15-month-old male Wistar rats. The animals were divided into 4 groups: 2 groups of intact animals of both ages (n = 12) and 2 groups of rats with isadrine model of heart failure (HF) (n = 10). HF was modeled by two subcutaneous injections of isoproterenol hydrochloride (170 mg/kg) at interval of 24 hours. Mitochondrial respiratory activity was assessed using respiratory control coefficient. The microviscosity of mitochondrial membranes was evaluated by eximerization coefficients of pyrene-based fluorescent probe in areas of protein-lipid and lipid-lipid contact. Comparative statistical analysis of independent groups was performed using the nonparametric Mann-Whitney test.Results. A decrease in mitochondrial respiratory control in older rats was shown in comparison with young animals. In the HF model, inter-age difference increases, but at the same time, in younger rats, the development of HF is not accompanied by significant changes in mitochondrial respiratory control. An age dependent decrease in the microviscosity of mitochondrial membranes in the area of protein-lipid and lipid-lipid interaction was revealed. In younger rats, the development of HF is characterized by a significant increase in microviscosity in the area of protein-lipid and lipid-lipid contact. In older rats, the development of the pathology is characterized by a significant decrease in microviscosity in the area of protein-lipid interaction.Conclusion. Multidirectional age-related changes in cardiomyocyte mitochondria of rats with heart failure were revealed. It was shown that mitochondria in younger rats retain their functional activity in the HF model unlike older rats.
Radial artery – perspective or fallacy?
Authors: Frolov A. V. 
Published: February 2023

Highlights. Radial artery is the second after internal thoracic artery by frequency of use among all of autogenous arterial conduits. Some modern studies indicate the superiority of this conduit to autogenous venous conduits; however, it remains an ongoing discussion.The article presence an analytical review regarding the use of the radial artery as one of conduits during coronary artery bypass grafting. Historical and contemporary perspectives of its use are given, examples of large-scale studies and results are provided, including the “graft-artery junction” concept.Abstract. Radial artery (RA) is the second after internal thoracic artery (ITA) by frequency of use among all of autogenous arterial conduits in coronary artery bypass grafting (CABG), however it is used in less than 13% of cases. The story of RA as a conduit starts in the 1970s, when the famous French surgeon, founder of modern mitral valve repair Alain Frédéric Carpentier proposed to use it. During the development of cardiovascular surgery, RA was actively introduced as a conduit for CABG, and nowadays there are both supporters and opponents of its application. On the one hand, long-term results of CABG with RA are superior to CABG with autogenous venous conduits, whereas the patency of RA is comparable with ITA. On the other hand, RA demonstrates worse angiographic indicators, which could be explained by a diffuse narrowing resulting in complete occlusion; such phenomenon is entitled “string-sign”. Moreover, as in case with other conduits, the site of proximal anastomosis, and harvesting method (open or endoscopic) play an important role in the long-term RA functioning and its resistance to atherosclerosis in grafted coronary arteries, thus making RA a good target for studing and predetermining new perspectives of its use.
Szabo versus provisional stenting of bifurcation coronary lesions
Authors: Kochergin N. A. , Sinkov M. A. , Vakkosov K. M. , Ganyukov V. I. 
Published: February 2023

Highlights. This article presents a comparative analysis of the results of PCI of bifurcation coronary lesions using the Szabo technique and provisional stenting in patients with chronic coronary artery disease.Background. Coronary bifurcation and ostial stenosis are difficult to treat and require precise stent positioning in order to prevent restenosis and thrombosis.Aim. To perform a comparative analysis of the outocmes of percutaneous coronary interventions (PCI) of coronary bifurcation lesions using the Szabo technique and provisional stenting in patients with chronic coronary artery disease.Methods. The retrospective single-center observational study included 791 patients. In accordance with the performed bifurcation PCI technique, the patients were divided into two groups: 42 patients in the Szabo group and 749 in the provisional stenting group. Propensity score matching was performed for endpoint analysis, as a result of which 42 patients were selected out of 749 patients in the provisional stenting group, comparable in baseline characteristics with 42 patients in the Szabo group. Study endpoints were cardiac death, myocardial infarction, and repeat revascularization. A composite endpoint (MACE) including all of the above was also evaluated.Results. The median follow-up in the provisional stenting and Szabo groups was 1.3 and 1.4 years, respectively. There were no significant differences between the groups in terms of mortality, myocardial infarction, repeated revascularization and composite endpoint (MACE 23.80% provisional stenting and 19.04% Szabo, p = 0.594).Conclusion. Thus, Szabo is technically simple and safe technique for stenting coronary bifurcation lesions in patients with chronic coronary artery disease, which is not inferior in terms of efficiency to provisional stenting.
Components of a healthy lifestyle among tertiary students in Kemerovo
Authors: Pomeshkina E. E. , Tsygankova D. P. , Bazdyrev E. D. 
Published: February 2023

Highlights. The results of an online survey of Kemerovo students regarding nutrition, physical activity, smoking, drinking alcohol, and maintaining a healthy lifestyle are presented.University students do not always follow the principles of healthy living due to poor nutrition, low physical activity and high consumption of tobacco and alcohol products.Aim. To evaluate dietary habits and modifiable cardiovascular risk factors in tertiary students in Kemerovo.Methods. The pilot study included 136 university students (aged 20-21 years) residing in Kemerovo who filled out online questionnaires. The questionnaires were comprised of questions about the preferred diet, lifestyle, level of physical activity, the presence of behavioral risk factors for non-communicable diseases (smoking, drinking alcohol). Additionally, the respondents` subjective opinion about their own healthy lifestyle (HLS) was evaluated.Results. The obtained data demonstrates a high prevalence of meat and sausage consumption (60%) and a low frequency of vegetables and fruits consumption (26%) among tertiary students. Simultaneously, most of the respondents have restricted the intake of savory snacks and consumption of sweet carbonated drinks. Almost a third of students skip breakfast and regularly visit fast food restaurants. Most of the respondents do not consider replacing red meat with fish or poultry, or using more low-fat dairy products and fruits instead of confectionery in their diet. A third of young people (33%) aged 20-21 have smoked or are currently smoking cigarettes, and more than half (63%) have consumed or regularly consume alcohol. Moreover, a very low percentage of students (7%) devote their time to regular physical activity. The analysis of the respondents` subjective opinion of their lifestyle has revealed that most students do not fully comprehend the concepts of “healthy lifestyle” and “healthy diet”, because their answers did not meet the criteria of healthy lifestyle. Only 57.7% of respondents who believe their lifestyle to be healthy are actually correct.Conclusion. Tertiary students in Kemerovo do not always follow the rules of healthy lifestyle. It has been illustrated by their poor nutrition, low physical activity and frequent alcohol and tobacco use. Students associate healthy diet exclusively with not consuming sugary drinks, savory snacks, fast food, and having proper breakfast, which could be caused by the lack of general knowledge about a proper balanced diet.
The role of mentoring in the formation and development of nurses' professional skills: Russian and world experience
Authors: Popsuyko A. N. , Danilchenko Y. V. , Andguladze O. P. , Artamonova G. V. 
Published: February 2023

Highlights. The level of professional competence of medical staff is important in rehabilitation of patients with cardiovascular diseases. It is important to build nurses' skills at all stages of professional development. The mentoring institute itself is integral to this process, as it ensures knowledge continuity and transfer of experience and best practices from more experienced staff to less experienced ones.Aim. To analyze existing mentoring practices.Methods. Using analytical method, content analysis and system analysis, we have analyzed domestic and foreign literature, as well as relevant regulatory and legal documentation for the period from 2017 to 2022.Results. The objectives of mentoring programs implemented in the practice of domestic and foreign healthcare organizations were analyzed. Existing approaches to its implementation in relation to the role of the mentoring institution in the formation and development of professional skills of nursing staff were evaluated.Conclusion. The analysis revealed a variety of approaches to the implementation of the mentoring programs for nursing staff. The study results highlight the significant role of mentoring in the formation of professional skills. It is proved that at each stage of mentoring process it is possible to gain knowledge and various skills necessary for medical staff to perform their duties independently.
Standardization & evaluation of anti-oxidant, anti-inflammatory and antimicrobial potential of Panchnimba Churna
Authors: Meena Ajay Kumar , Rajput Sanjana , Chaturvedi Sadhana , Parashar Deepesh , Sharma Lavkush 
Published: February 2023

: Ayurvedic medication pancha nimba choorna is a herbal powder. The panchanimba is a well-known medication stated in the old Ayurvedic texts used to cure kshudra kushtas (minor skin disorders), such as eczema, acne, and Maha kushtas (major skin ailments).It is used to treat a variety of skin conditions, including dermatitis, pruritis, psoriasis, sinus, non-healing wounds, headaches, uti, diabetes, toxins, and arthritis. It is employed to treat infections of the skin.The traditional use of the neem tree's leaves, blossoms, seeds, fruits, roots, and bark for the treatment of inflammation, infections, fever, skin conditions, and dental problems includes all of these parts. Neem leaf's therapeutic benefits have been specifically discussed. The immunomodulatory, anti-inflammatory, anti-hyperglycaemic, antiulcer, antimalarial, antifungal, antibacterial, antiviral, antioxidant, antimutagenic, and anticarcinogenic activities of neem leaf and its components have been proven. Protein, carbohydrates, flavonoids, phenol, saponin, glycosides, tannin, and alkaloids are all present according to phytochemical analysis, whereas terpenoids, sterols, and other substances are not present.To guarantee the quality of the product, standardization employing various parameters was used which was performed as per the Indian Pharmacopoeia, WHO guidelines and various literature reports. The antioxidant activity may be attributes to flavonoids and phenolics present in extracts of . In terms of gallic acid equivalent, Panchanimb Churna contained 105 mg/g of total phenols and the total flavonoid concentration was 43.8214 mg/g, which is the quercetin equivalent.Antioxidant property was evaluated using DPPH activity.According to a forementioned quality criterion, Panchanimb Churna was determined to be competent under every examined aspect. The current study strengthens belief in herbal remedies of natural origin to combat infectious diseases and other illnesses that may be included in medications in the future. Overall the product is very beneficicial in day to day life for humans as it possess various qualities and activities.
Pattern and outcome of obstetric patients admitted in the medical critical care unit of tertiary care hospital
Authors: Shetti Akshaya Narayan , Patel Arush , Mustilwar Rachita G 
Published: February 2023

Various types of diseases are seen in pregnant patients, it may be prior to the pregnancy or during the pregnancy period. Certain diseases may lead to critical care admission and may be either elective or emergency admission. This study was conducted to know the pattern and outcome of obstetric patients getting admitted to the medical critical care unit of our tertiary care hospital. This is a retrospective study, and we collected the data from the records for two years duration, starting from 1st January 2020 to 31st December 2021. A total of 97 patients were admitted and a maximum number of patients were aged between 18-25 years. 56(57.7%) of patients were primigravida, and 58(59.8%) of the patients were registered cases. 82(84.5%) of cases were registered under the medico-legal case as per Indian law. 68(70.1%) patients had lower segment cesarean sections. Among 97 patients, 22(22.7%) of patients had comorbid illnesses. A total of 22(22.7%) patients got admitted for poisoning-related issues, which was the single most common cause among 97 admissions. 25(25.8%) of patients were on mechanical ventilation and the average number of critical care unit stay was 4 days. 65(67%) patients survived, 15(15.5%) patients died, and the remaining patient was either took discharged against the advice or referred.Obstetric patient admission in the rural critical care unit is not uncommon, and poisoning is the most common cause of admission in the medical critical care unit.
Critical Discourse Analysis on the Instagram Account Mubadalah.id from the Perspective of Islamic Feminism
Authors: Rohmah Sitti , Suyanto Budi 
Published: February 2023

This study aims to analyze the macro structure, super structure, and micro structure contained in the media text Mubilah.id. The research method used is descriptive qualitative method with Teun's perspective. A. Van Dijk. The data collection used in this study is the listening method with reading techniques and note taking techniques. The results of the data analysis in this study are (1) the macro structure depicts how the act of polygamy in Islam is not recommended; (2) the superstructure in the study shows that the data is preceded by the title, opening text, news content, and closing section; and (3) The micro structure in this study consists of semantics in which there is a background in the form of before marriage, details indicating media control or emphasis on decisions in an agreement made before marriage, the intent/implicature is that marital relations may not create domination of rights in one of them. between the two, and the presupposition that arises is that women have the full right to decide or refuse polygamy. Furthermore, the syntax which consists of coherence, cause and effect to get the dream marriage, namely by establishing communication by submitting conditions or prenuptial agreements and stylistics in the form of the right choice of words is marriage. All data contains views of Islamic feminism which are built not to favor only one gender. However, giving a broad view to glorify fellow human beings.
Keywords : Acts of Violence, Agreement, Gender, Marriage, and Mubadalah.id
Entrevista de Julia Monteiro Viana com a Professora Maria Lucia Lee (Inverno, 2022)
Authors: Viana Julia Monteito 
Published: February 2023

Maria Lucia Lee é formada em Física na Faculdade de Ciências da USP (1972) trabalhou como analista de sistemas até o ano de 1980 quando decidiu se dedicar às artes corporais terapêuticas da Medicina Tradicional Chinesa (MTC). Desde então transmite, pesquisa e estuda estas artes. A presente entrevista trata de questões desenvolvidas a partir da convocatória da Revista Conceição para criação de um dossiê sobre ancestralidade e cosmopercepções. Através de uma conversa fluida, a entrevistada conta sobre seus processos de vida, a história de sua família e sua trajetória de pesquisa e prática dentro do campo da Medicina Tradicional Chinesa.