bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal miR‐205‐5p derived from periodontal ligament stem cells attenuates the inflammation of chronic periodontitis via targeting XBP1
Authors: Kang Lixun , Miao Yibin , Jin Ying , Shen Siyu , Lin Xiaoping 
Published: December 2022

AbstractIntroductionChronic periodontitis (CP) is an inflammatory periodontal disease with high incidence and complex pathology. This research is aimed to investigate the function of exosomal miR‐205‐5p (Exo‐miR‐205‐5p) in CP and the underlying molecular mechanisms.MethodExo‐miR‐205‐5p was isolated from miR‐205‐5p mimics‐transfected periodontal ligament stem cells (PDLSCs), and subsequently cocultured with lipopolysaccharide (LPS)‐induced cells or injected into LPS‐treated rats. The mRNA expression of inflammatory factors and Th17/Treg‐related factors were measured by quantitative real‐time PCR. The contents of inflammatory factors and the percentages of Th17/Treg cells were measured by enzyme‐linked immunosorbent assay and flow cytometry, respectively. Besides, the target relation between miR‐205‐5p and X‐box binding protein 1 (XBP1) was explored.ResultsMiR‐205‐5p was downregulated in LPS‐induced PDLSCs and corresponding exosomes. Exo‐miR‐205‐5p inhibited inflammatory cell infiltration, decreased the production of TNF‐α, IL‐1β, and IL‐6, and decreased the percentage of Th17 cells in LPS‐treated rats. In addition, XBP1 was a target of miR‐205‐5p. Overexpression of XBP1 weakened the effects of Exo‐miR‐205‐5p on inhibiting inflammation and regulating Treg/Th17 balance in LPS‐induced cells.ConclusionsExo‐miR‐205‐5p derived from PDLSCs relieves the inflammation and balances the Th17/Treg cells in CP through targeting XBP1.
Epitope identification for p53R273C mutant
Authors: Zhang Jian , Liu Minglu , Chen Yin , Zhou Zishan , Wang Ping , Yu Yang , Jiao Shunchang 
Published: December 2022

AbstractBackgroundWith the rise of immunotherapy based on cancer neoantigen, identification of neoepitopes has become an urgent problem to be solved. The TP53 R273C mutation is one of the hotspot mutations of TP53, however, the immunogenicity of this mutation is not yet clear. The aim of this study is to identify potential epitopes for p53R273C mutant.MethodsIn this study, bioinformatic methods, peptide exchange assay, and peptide‐immunized human leukocyte antigen (HLA) transgenic mouse model were used to explore the immunogenicity of this mutation.ResultsPeptides with higher affinity to common HLA‐A alleles (A*11:01, A*02:01) were discovered by computational prediction. All the 8–11 mer peptides contain the mutation site were synthesized and soluble peptides were used in the peptide exchange assay. However, the exchange efficiencies of these predicted peptides to HLAs were lower. Fortunately, other peptides with higher exchange efficiency were discovered. Then, the immunogenicity of these peptides was validated with the HLA‐A2 transgenic mice model.ConclusionWe identified three potential neoepitopes of p53R273C for HLA‐A*02:01, one potential neoepitope for HLA‐A*11:01 and no neoepitope for HLA‐A*24:02.
Shifting incidence and survival of epithelial ovarian cancer (1995‐2014): A SurvMark‐2 study
Authors: Cabasag Citadel J. , Arnold Melina , Rutherford Mark , Ferlay Jacques , Bardot Aude , Morgan Eileen , Butler John , O'Connell Dianne L. , Nelson Gregg , Høgdall Claus , Schnack Tine , Gavin Anna , Elwood Mark , Hanna Louise , Gourley Charlie , De Prithwish , Saint‐Jacques Nathalie , Mørch Lina Steinrud , Woods Ryan R , Altman Alon D. , Sykes Peter , Cohen Paul A. , McNally Orla , Møller Bjørn , Walsh Paul , Morrison David S. , Bray Freddie , Soerjomataram Isabelle 
Published: December 2022

AbstractThe aim of the study is to provide a comprehensive assessment of incidence and survival trends of epithelial ovarian cancer (EOC) by histological subtype across seven high income countries (Australia, Canada, Denmark, Ireland, New Zealand, Norway and the United Kingdom). Data on invasive EOC diagnosed in women aged 15 to 99 years during 1995 to 2014 were obtained from 20 cancer registries. Age standardized incidence rates and average annual percentage change were calculated by subtype for all ages and age groups (15‐64 and 65‐99 years). Net survival (NS) was estimated by subtype, age group and 5‐year period using Pohar‐Perme estimator. Our findings showed marked increase in serous carcinoma incidence was observed between 1995 and 2014 among women aged 65 to 99 years with average annual increase ranging between 2.2% and 5.8%. We documented a marked decrease in the incidence of adenocarcinoma “not otherwise specified” with estimates ranging between 4.4% and 7.4% in women aged 15 to 64 years and between 2.0% and 3.7% among the older age group. Improved survival, combining all EOC subtypes, was observed for all ages combined over the 20‐year study period in all countries with 5‐year NS absolute percent change ranging between 5.0 in Canada and 12.6 in Denmark. Several factors such as changes in guidelines and advancement in diagnostic tools may potentially influence the observed shift in histological subtypes and temporal trends. Progress in clinical management and treatment over the past decades potentially plays a role in the observed improvements in EOC survival.
Twelve Months of Denosumab and/or Alendronate Is Associated With Improved Bone Fatigue Life, Microarchitecture, and Density in Ovariectomized Cynomolgus Monkeys
Authors: Haider Ifaz T. , Loundagin Lindsay L. , Sawatsky Andrew , Kostenuik Paul J. , Boyd Steven K. , Edwards W. Brent 
Published: December 2022

ABSTRACTProlonged use of antiresorptives such as the bisphosphonate alendronate (ALN) and the RANKL inhibitor denosumab (DMAb) are associated with rare cases of atypical femoral fracture (AFF). The etiology of AFF is unclear, but it has been hypothesized that potent osteoclast inhibitors may reduce bone fatigue resistance. The purpose of this study was to quantify the relationship between antiresorptive treatment and fatigue life (cycles to failure) in bone from ovariectomized cynomolgus monkeys. We analyzed humeral bone from 30 animals across five treatment groups. Animals were treated for 12 months with subcutaneous (sc) vehicle (VEH), sc DMAb (25 mg/kg/month), or intravenous (iv) ALN (50 μg/kg/month). Another group received 6 months VEH followed by 6 months DMAb (VEH‐DMAb), and the final group received 6 months ALN followed by 6 months DMAb (ALN‐DMAb). A total of 240 cortical beam samples were cyclically tested in four‐point bending at 80, 100, 120, or 140 MPa peak stress. High‐resolution imaging and density measurements were performed to evaluate bone microstructure and composition. Samples from the ALN (p = 0.014), ALN‐DMAb (p = 0.008), and DMAb (p < 0.001) groups illustrated higher fatigue‐life measurements than VEH. For example, at 140 MPa the VEH group demonstrated a median ± interquartile range (IQR) fatigue life of 1987 ± 10593 cycles, while animals in the ALN, ALN‐DMAb, and DMAb groups survived 9850 ± 13648 (+395% versus VEH), 10493 ± 16796 (+428%), and 14495 ± 49299 (+629%) cycles, respectively. All antiresorptive treatment groups demonstrated lower porosity, smaller pore size, greater pore spacing, and lower number of canals versus VEH (p < 0.001). Antiresorptive treatment was also associated with greater apparent density, dry density, and ash density (p ≤ 0.03). We did not detect detrimental changes following antiresorptive treatments that would explain their association with AFF. In contrast, 12 months of treatment may have a protective effect against fatigue fractures. © 2022 American Society for Bone and Mineral Research (ASBMR).
Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)
Authors: Błaszczyk Florentyna , Sosinka Agnieszka , Wilczek Grażyna , Student Sebastian , Rost‐Roszkowska Magdalena 
Published: December 2022

AbstractAdult specimens or larvae of invertebrates used as food for vertebrates are often maintained close to gluten so they might become vectors for cereal proteins. However, the tissues and internal organs can respond differently in animals with different feeding habits. The midgut epithelium might be a first and sufficient barrier preventing uptake and effects of gluten on the whole body, while the fat body is the main organ that accumulates different xenobiotics. Good models for such research are animals that do not feed on gluten‐rich products in their natural environment. The project's goal was to investigate alterations in the midgut epithelium and fat body of the herbivorous millipede Telodeinopus aoutii (Diplopoda) and analyze cell death processes activated by gluten. It enabled us to determine whether changes were intensified or reversed by adaptive mechanisms. Adult specimens were divided into control and experimental animals fed with mushrooms supplemented with gluten and analyzed using transmission electron microscopy, flow cytometry, and confocal microscopy. Two organs were isolated for the qualitative and quantitative analysis: the midgut and the fat body. Our study of the herbivorous T. aoutii which does not naturally feed on gluten containing diet showed that continuous and prolonged gluten feeding activates repair processes that inhibit the processes of cell death (apoptosis and necrosis) and induce an increase in cell viability.
Use of probiotic yeasts with biocontrol activity for fermentation of ewe's milk
Authors: Fernández‐Pacheco Pilar , Ramos Monge Inés María , Poveda Justa María , Díaz‐Maroto M. Consuelo , Arévalo‐Villena María 
Published: December 2022

AbstractBACKGROUNDThere are sufficient scienctific studies that support the benefit that fermented dairy products produce in those who consume them. Traditionally, cow's milk has been the most commonly used milk but there is a growing interest in the development of new dairy products, substituting cow's milk with milk from other sources, as well as in the use of microorganisms in fermentation to replace artificial preservatives or treatments that may affect the chemical and organoleptic characteristics of the product. For these reasons, the aim of the present work was to understand the behavior of five potential probiotic yeasts during the fermentation of ewe's milk and to consider their potential use as biocontrol agents.RESULTSSaccharomyces cerevisiae 3 and Hanseniaspora osmophila 1056 provided the most promising kinetic parameters in the different salt, temperature and pH conditions tested in their technological characterization. The profiles of organic acids and volatile compounds after the fermentation period was noteworthy for contributing to the final aroma of the dairy product. Sensory analysis revealed the sour taste of all samples, and S. cerevisiae 3, Lachancea thermotolerans 1039, and H. osmophila 1056 stood out for an accentuated cheese flavor. In addition, all strains showed biocontrol activity; they reduced the mycelium of the mycotoxigenic molds.CONCLUSIONSaccharomyces cerevisiae 3 and H. osmophila 1056 could be inoculated along with bacterial starters to provide a functional fermented beverage with improved flavor. These strains also have an added value as they act as biocontrol agents. © 2022 Society of Chemical Industry.
Exploiting Desired Phosphor‐In‐Glass for All‐Inorganic Solid‐State White Illumination
Authors: Bao Shuyang , Liang Yueyuan , Zhao Cong , Wang Luhan , Liang Xiaojuan , Xiang Weidong 
Published: December 2022

AbstractSolid‐state white lighting applications with high brightness, low power consumption and long operating life have gradually dominated the modern industrial illumination market. For applications in white light‐emitting diodes (WLEDs) and white‐lighting laser diodes (WLDs), Phosphor‐in‐Glass (PiG), owing to its facile process, high efficiency and excellent chromaticity tunability, stands out as an optimal choice among various types of color converters. Currently, research in yellow‐emitting Y3Al5O12:Ce3+ (YAG: Ce) phosphors combined with different glass system has reached a mature stage and the poor illumination is a major bottleneck. Moreover, to realize high‐quality white lighting, exploiting full‐color PiGs is of great importance for its commercialization. Herein, the multicolour PiGs their successfully obtained by deliberately matching different color‐emitting phosphors with the glass matrix. Glass composition selection rules for specific phosphors their proposed based on investigations of glass protection mechanism and the corrosion reaction between phosphors and glass matrix. In addition, an astonishing YAG: Ce PiG phenomenon (zero thermal quenching) is founded and discussed. As for practical applications, high‐quality 5‐W chip‐on‐board (COB) WLEDs their constructed by utilizing simple matches of colorful PiGs. Assembled WLDs modules also rendered a high‐efficiency and stable lighting source. These results dramatically inspire the development of high‐quality solid‐state white lighting applications.
Synergistic Effects of Surface Ligand Engineering and Double Matrices Protecting Enable Ultrastable and Highly Emissive Blue/Cyan Perovskite Nanocrystal Films for Multifunctional Applications
Authors: Hu Xiaobo , Xu Yanqiao , Wang Jiancheng , Ma Jiaxin , Wang Lianjun , Jiang Wan 
Published: December 2022

AbstractPerovskite nanocrystals (NCs) have attracted considerable attention because of the extraordinary optoelectronic properties, making them promising candidates for lighting and display applications. However, the emissive efficiency and stability of blue/cyan‐emitting NCs lag far behind other color emissive perovskites, which greatly restrict their practical application. Herein, a feasible strategy is proposed for synthesizing highly luminescent and stable CsPbClxBr3−x NCs by the didodecyldimethylammonium bromide (DDAB) ligand‐assisted post‐treatment. The prepared DDAB‐CsPbCl0.8Br2.2 and DDAB‐CsPbCl2.0Br1.0 NCs exhibit bright cyan and blue emission, respectively, and their quantum yields are significantly enhanced from 12.2% and 7.5% to 83.1% and 75.3% owing to the obvious decrease of surface defects by the ligand‐exchange and halogen‐compensation mechanisms. The outstanding fluorescence properties are almost remained by grafting perovskite NCs onto the surface of SiO2 nanospheres. Notably, the ultrahigh water‐stability is realized after encapsulating the composite nanospheres into polystyrene‐polyisoprene‐polystyrene (SIS) film, which is benefit from the triple protection of DDA+ cations, SiO2 nanospheres and SIS copolymers. The fabricated SiO2@DDAB‐CsPbCl2.0Br1.0/SIS film is assembled into an white light emitting diode with high color rendering index of 83.4. Importantly, the blue/cyan‐emitting films display superb temperature‐sensitivity (30–160 °C) and favorable elasticity (1200% stretch rate), indicating their promising application prospect in flexible multifunctional photoelectric devices.
Emerging Trends of J‐Aggregate Formation within Polymeric Nanoassemblies
Authors: Hou Mingxuan , Liu Shiyong 
Published: December 2022

AbstractJ‐aggregation of dyes is a unique type of fluorescence aggregation phenomenon exhibiting bathochromic shift and higher absorption coefficient compared to the nonaggregated monomeric form. The incorporation of J‐aggregation dyes with polymeric nanoassemblies endows the former with enhanced microstructural stability and aqueous dispersity, as well as functional integration. Importantly, near‐infrared (NIR) polymeric J‐aggregates have been widely used for in vivo imaging and therapeutic functions. This perspective aims to discuss recent trends of J‐aggregate formation within polymeric nanoassemblies either covalently conjugating or physically encapsulating specific fluorescent dyes. Current challenges and future opportunities are also summarized for the subfield of polymeric J‐aggregates.
Automated detection of toxicophores and prediction of mutagenicity using PMCSFG algorithm
Authors: Schietgat Leander , Cuissart Bertrand , De Grave Kurt , Efthymiadis Kyriakos , Bureau Ronan , Crémilleux Bruno , Ramon Jan , Lepailleur Alban 
Published: December 2022

AbstractMaximum common substructures (MCS) have received a lot of attention in the chemoinformatics community. They are typically used as a similarity measure between molecules, showing high predictive performance when used in classification tasks, while being easily explainable substructures. In the present work, we applied the Pairwise Maximum Common Subgraph Feature Generation (PMCSFG) algorithm to automatically detect toxicophores (structural alerts) and to compute fingerprints based on MCS. We present a comparison between our MCS‐based fingerprints and 12 well‐known chemical fingerprints when used as features in machine learning models. We provide an experimental evaluation and discuss the usefulness of the different methods on mutagenicity data. The features generated by the MCS method have a state‐of‐the‐art performance when predicting mutagenicity, while they are more interpretable than the traditional chemical fingerprints.
Multiple sessions of therapeutic electrical stimulation using implantable thin‐film wireless nerve stimulators improve functional recovery after sciatic nerve isograft repair
Authors: Birenbaum Nathan K. , Yan Ying , Odabas Arman , Chandra Nikhil S. , Ray Wilson Z. , MacEwan Matthew R. 
Published: December 2022

AbstractIntroduction/AimsAlthough therapeutic electrical stimulation (TES) of injured peripheral nerve promotes axon regeneration and functional recovery, clinical applications of this therapy are limited to the intraoperative timeframe. Implantable, thin‐film wireless nerve stimulators offer a potential solution to this problem by enabling delivery of electrical stimuli to an injured nerve over a period of several days post‐surgery. The aim of this study was to determine the optimal time course of stimulation for maximizing functional recovery in a rat sciatic nerve isograft repair model.MethodsAdult male Lewis rats underwent thin‐film wireless nerve stimulator implantation following sciatic nerve transection and 40 mm nerve isograft repair. Immediately after surgery, animals began a daily regimen of TES for up to 12 consecutive days. Functional recovery was assessed by compound muscle action potential (CMAP), evoked muscle force, wet muscle mass, and axon counting.ResultsSerial CMAP measurements increased in amplitude over the course of the study, yet no significant difference between cohorts for serial or terminal CMAPs was observed. Axon counts and wet muscle mass measurements were greatest in the 6‐day stimulation group, which correlated with a significant increase in evoked muscle force for the 6‐day stimulation group at the terminal time point.DiscussionSix daily sessions of TES were found to be most effective for augmenting functional recovery compared to other time courses of stimulation. Future studies should incorporate additional subjects and track axonal sprouting or measure neurotrophin levels during the therapeutic window to further elucidate the mechanisms behind, and ideal amount of, TES.
Children's engineering‐related achievement beliefs and career aspirations: The role of gender
Authors: Woods Bobbi , Miller Cindy Faith , Wheeler Lorey A. , Reisslein Martin 
Published: December 2022

AbstractThis study examined the association between elementary students' (N = 1627; 51.3% girls, ages 4–12) engineering‐related ability beliefs (competence), task‐value beliefs (interest, importance), and career aspirations. Findings suggest that mean levels of children's engineering‐related beliefs did not vary by gender. High levels of competence, interest, and importance beliefs were related to higher engineering‐related career aspirations. Findings also revealed that the association of competence and interest with career aspirations was stronger for girls than boys; whereas, the association of importance with career aspirations was stronger for boys than girls. Last, results provide evidence of importance as a moderator of the link between competence and career aspirations, suggesting that there was a stronger positive association between competence and career aspirations under high levels of importance. Against the backdrop of the increasing urgency to teach engineering in elementary schools, this study highlighted the salience of examining children's engineering‐related motivational beliefs. The study has practical implications for teachers and school psychologists on promoting inclusive instruction in elementary schools. Specifically, the implications of the results for instructional strategies as well as assessments and interventions in elementary schools are discussed.
Clinical manifestations of EVALI in adolescents before and during the COVID‐19 pandemic
Authors: Abdallah Bayan , Lee Harin , Weerakoon Sitara M. , Messiah Sarah E. , Harrell Melissa B. , Rao Devika R. 
Published: December 2022

AbstractIntroductionE‐cigarette, or vaping, product use‐associated lung injury (EVALI) results from inhaling the aerosol of e‐cigarettes and has similar clinical features to coronavirus disease 2019 (COVID‐19). EVALI case counts since the declaration of the COVID‐19 pandemic is unknown.MethodsA retrospective electronic health record chart review of adolescents hospitalized at one institution with EVALI was conducted. Clinical characteristics and hospital course of patients hospitalized during the pandemic were compared to those prepandemic.ResultsThe clinical presentation of adolescents hospitalized prior‐to (n = 19) and during the COVID‐19 pandemic (n = 22) were similar with respect to constitutional, respiratory, and gastrointestinal symptoms. All patients hospitalized during the pandemic were tested for COVID‐19 at least once. Only one patient had a positive SARS‐CoV‐2 RT‐PCR test result. Thirty‐one out of 39 patients treated with corticosteroids had clinical improvement within 24 h (79%). Patients hospitalized during the pandemic had a shorter median length of stay (5 vs. 7 days, p < 0.01), and were less often discharged with home oxygen (1 vs. 6 patients, p = 0.04). Pulmonary function tests improved pre‐ to postcorticosteroid treatment and postcorticosteroid to follow‐up.ConclusionsEliciting a history of vaping in adolescents presenting with constitutional, respiratory, and gastrointestinal symptoms is important to identify EVALI cases, which have continued throughout the COVID‐19 pandemic. A shorter length of stay with less need for mechanical ventilation and home oxygen in adolescents hospitalized during the pandemic may reflect increased familiarity with EVALI characteristics. Corticosteroids led to clinical and pulmonary function improvement.