bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two novel single-chain variable fragments exert bactericidal activity against Acinetobacter baumannii through destabilization of the outer membrane
Authors: Basardeh Eilnaz , Gavgani Somayeh Piri , Nikbin Vajihe sadat , Nazari Farzaneh , Oghalaie Akbar , Azizi Masoumeh , Sharifzadeh Zahra , Fateh Abolfazl , Rahimi-Jamnani Fatemeh 
Published: May 2023

Abstract
Background Acinetobacter baumannii is notorious for its high levels of resistance and the development of clinically-effective antimicrobial agents seems to be an urgent medical challenge. Bactericidal single-chain variable fragments (scFvs) are novel antibacterial agents capable of inhibiting the growth of pathogens (e.g. Staphylococcus aureus, Pseudomonas aeruginosa, A. baumannii) independently of the host immune system. We previously found that two fully human scFvs, EB211 and EB279, showed direct growth inhibitory activity against A. baumannii strains. In the current study, the antibacterial activity of EB211 and EB279 against A. baumannii, Klebsiella pneumonia, and P. aeruginosa strains was appraised in the presence of a high concentration of magnesium (Mg2+) (20 mM) to find the bactericidal mechanism of these two scFvs. Moreover, epitope mapping and immunoblotting were done to identify A. baumannii proteins targeted by EB211 and EB279.
Results EB211 and EB279 similar to colistin sulfate, lost their activity in the presence of Mg2+, demonstrating that EB211 and EB279 exerted growth inhibitory activity by displacing Mg2+ and interrupting the integrity of the outer membrane. The immunoblotting results also exhibited that EB211 and EB279 bound OprD and TonB family C-terminal domain, respectively.
Conclusions EB211 and EB279 by disruption of the outer membrane A. baumannii elicit direct growth inhibitory activity without the need for immune cells or complements which could be helpful for use in immunocompromised patients.
Development of a Loop-Mediated Isothermal Amplification Assay for Rapid and sensitive Detection of Hemileia vastatrix in Coffee
Authors: Wu Weihuai , Wang Guihua , Wang Han , Gbokie Thomas , He Chunping , Huang Xing , Liang Yanqiong , Li Rui , Yi Kexian 
Published: May 2023

Abstract
Coffee leaf rust (CLR) caused by Hemileia vastatrix is a worldwide devastating disease. Early monitoring is crucial for controlling CLR quickly and efficiently. However, it is difficult to accurately identify CLR at the early stage via naked eye; and the detection of H. vastatrix using PCR-based methods were time-consuming, laborious, and sometimes low- sensitivity. LAMP technology is known for its speed, specificity, and sensitivity to accurately identify many different pathogens. Therefore, in this study, by comparing ITS sequences of H. vastatrix with other H. vastatrix and Uredinales strains using National Center for Biotec- hnology Information (NCBI) by BLASTn, we designed specific primers targeting the unique region and its flanking regions of ITS sequences for H. vastatrix. Using SYBR Green I dye, we established a LAMP technique for rapid and sensitive detection of H. vastatrix. Furthermore, we optimized the LAMP protocol was to enhance both sensitivity and specificity for H. vastatrix detection. Under the optimized condition, the establised LAMP protocol could detect as low as 1pg/μl of H. vastatrix DNA in 60min at 63℃, which is ~100 times more sensitive than conventional PCR. This method can successfully detect H. vastatrix in the early CLR symptom on the coffee leaves in the field.
Immune checkpoint inhibitors combined with radiotherapy/chemoradiotherapy in treating locally advanced or recurrent/metastatic esophageal squamous cell carcinoma: A real-world study
Authors: Zhao Xiao-Han , Gao Hong-mei , Wen Jing-Yuan , Wang He-Song , Wu Luan-ying , Song Chun-Yang , Deng Wen-Zhao , Zhu Shu-Chai , Shen Wen-Bin 
Published: May 2023

Abstract
Objective: This study was designed to investigate the prognostic factors for immune checkpoint inhibitors (ICIs) combined utilization with radiotherapy (RT)/chemoradiotherapy (CRT) and to evaluate their toxicity in locally advanced or recurrent/metastatic esophageal squamous cell carcinoma (LA/RM ESCC).
Methods: In this study, 198 LA/RM ESCC patients who received ICIs combined with RT/CRT in the Department of Radiotherapy of the Fourth Hospital of Hebei Medical University were retrospectively analyzed. Univariate and multivariate analyses were performed to determine the prognostic factors for overall survival (OS) and progression-free survival (PFS). Subgroup analysis was utilized to explore the prognostic factors, the treatment respond and treatment-related adverse events (trAEs) were analyzed.
Results: The median OS and PFS were 30.4 months (95% confidence interval [CI]: 15.1–45.7 months) and 15.3 months (95% CI: 12.8–17.8 months), respectively. The median OS and PFS for patients achieving objective respond (ORR group, including complete response and partial response) were 50.8 months (95% CI: 25.8–75.7 months) and 20.5 months (95% CI: 14.1–27.0), respectively, which were higher than those in non-ORR group (OSnon-ORR:17.5 months, 95% CI: 14.0–21.0; χ2 = 13.881, P < 0.001; PFSnon-ORR: 12.1 months, 95% CI: 10.1–14.1, χ2 = 10.676, P = 0.001). Results from subgroup analysis illustrated combined ICIs with radiotherapy could improve the treatment respond (χ2 = 47.725, P = 0.000). The OS rate (χ2 = 18.836, P < 0.001) and PFS rate (χ2 = 6.881, P = 0.009) were significantly higher in the whole-lesion radiotherapy group than in the partial-lesion radiotherapy group. Multivariate analysis showed that the number of immune cycles, the coverage of radiotherapy target, the modality of ICIs-combination therapy and treatment response were independent prognosis factors for OS (hazard ratio [HR] = 0.512, 2.043, 1.889, and 1.912, respectively; P = 0.001, 0.001, 0.013, and 0.002, respectively). Radiotherapy coverage and treatment response were independent factors for PFS (HR = 1.478 and 1.597, respectively; P = 0.032 and 0.012, respectively). In the entire study population, 83 patients (41.9%) had ≥ grade 2 trAEs.
Conclusions: ICIs combined with radiotherapy/chemoradiotherapy are safe and effective in LA/RM ESCC patients. The addition of radiotherapy could improve the treatment respond and whole-lesion radiotherapy improve prognosis compared with partial-lesion radiotherapy group. The number of immunotherapy cycles and treatment response are the main factors affecting prognosis.
Pay-it-forward to increase uptake among 15-18-year-old adolescent girls compared with user-paid vaccination: The pilot results of a two-arm randomized controlled trial in China
Authors: Qin Chuanyu , Li Yifan , Qiu Shengyue , He Yu , Yim Vivian Wan-Cheong , Tang Shenglan , Du Heng , Gong Wenfeng , Tucker Joseph D , Tang Weiming , Lin Leesa , Smith Jennifer , Wu Dan , Li Jing 
Published: May 2023

Abstract
Background
China has low human papillomavirus(HPV) vaccination rate due to lack of public funding and mistrust in domestic vaccines. This pilot study evaluated the feasibility and preliminary effectiveness of an innovative pay-it-forward strategy, which has a participant receive a subsidized vaccine and offers her an opportunity to donate to support other girls, in improving HPV vaccine uptake among 15-18-year-old adolescent girls.
Methods
A two-arm randomized controlled pilot trial was performed in one vaccination clinic in Western China. Adolescent girls (via caregivers) were invited to participate the study via online dissemination of the pilot study. Eligible ones were then randomly allocated using a sealed envelope method into standard-of-care or pay-it-forward arm in a 1:1 ratio. Pay-it-forward participants received hand-written postcard messages, a subsidized vaccine, and an opportunity to donate and (or) write postcards for future recipient girls. Standard-of-care participants self-paid for vaccines. The primary outcome was the first-dose HPV vaccine uptake, which was calculated using multivariable logistic regression, presented as crude/adjusted odds ratios (cORs/aORs) and 95% confidence intervals (CIs). Standard scales were used to assess the feasibility of the program.
Results
A total of 100 participants (50 in each arm) were recruited from Jan 4, 2022 to Feb 18, 2022. The HPV vaccine uptake rate was 98% (49/50) in the pay-it-forward arm and 82% (41/50) in the standard-of-care arm (cOR = 10.76, 95% CI: 1.31–88.47, P = 0.027; aOR = 12.12, 95% CI: 1.37-107.29, P = 0.025). The completion rate of full schedule of HPV vaccination in the two arms was 100% (49/49) and 95.1% (39/41), respectively. Of all 49 vaccinated girls in the pay-it-forward arm, 38 (77.6%) donated to support future participants, and the total donation accounted for 33.3% of prepaid subsidization. Among caregivers in the pay-it-forward arm, 97.6% (41/42) believed that this strategy was feasible.
Conclusions
The pilot showed feasibility and preliminary effectiveness of a pay-it-forward strategy to increase HPV vaccination rate. The high uptake rate in the standard-of-care arm is likely caused by the selection bias associated with the online dissemination approach and secured vaccine supply through the program. Further adaption of the intervention package and a population-based recruitment pathway are needed to better reflect local contexts and enhance the generalizability of the subsequent formal trial.
Trial registration
Chinese Clinical Trial Registry (ChiCTR), ChiCTR2200055542. Retrospectively registered on 11 January 2022, https://www.chictr.org.cn/showproj.html?proj=139738.
How to develop accessible Information Systems: a practical case in tourism and proposal of a roadmap to support the development of accessible solutions
Authors: Teixeira Pedro , Teixeira Leonor , Eusébio Celeste 
Published: May 2023

Abstract
Purpose
The right to tourism and leisure has become a crucial aspect of society. In that sense, through more accessible tourism, it is possible to improve travel conditions for people with disabilities (PwD). Nonetheless, barriers still exist, with the lack of information about accessibility conditions, as well as communication accessibility itself, representing some of the main obstacles. Information systems (IS) can help overcome these hurdles. However, it is verified, that methodologies to support the development of accessible IS are currently very scarce. Thus, this study intends to develop an accessible IS for accessible tourism and propose a roadmap to support the creation of accessible IS solutions.
Methods
To obtain the intended accessible tourism solution, an action research methodology was followed, which involved adapting already established frameworks, that combine Agile development and user-centered design techniques. Adjustments were made to incorporate accessibility, taking into consideration the requirements of the accessible tourism market.
Results
In this study, a web application named access@tour by action was created. This mobile solution is capable of improving information management within the accessible tourism market. From this experimental study, a proposal for a methodological roadmap emerged. This roadmap helps better understand how to develop accessible IS, by demonstrating techniques for gathering accessibility requirements and validating them.
Conclusion
The access@tour by action is a much-needed solution, to enhance accessible tourism conditions. The roadmap is adaptable and suitable for IS projects involving accessibility, regardless of the area. Both results provide a better perspective on how to integrate accessibility during the development of IS, possibly supporting future researchers in creating accessible solutions.
Suppressed Ion Migration for High-performance X-ray Detectors Based on Atmosphere-Controlled EFG-Grown CsPbBr3 Single Crystals
Authors: Hua Yunqiu , Zhang Guodong , Sun Xue , Li Xiang , Zhai Zhongjun , Cui Fucai , Liu Jiaxin , Liu Hongjie , Tao Xutang 
Published: May 2023

Abstract

Halide perovskites have shown great potential for X-ray detection in medical imaging and product inspection applications. However, the ion migration in lead halide perovskites, which is usually affected by crystal defects, causes large noise and baseline drift, deteriorating the X-ray detection and imaging performance. In this work, we adopt the atmosphere-controlled edge-defined film-fed growth (EFG) method to grow high-quality shape-controlled CsPbBr3 single crystals (SCs) in an Ar and HBr mixed atmosphere for the first time. Compared with the vertical Bridgman (VB)-CsPbBr3 SCs, the EFG-CsPbBr3 SCs show a much lower trap density (4.24 × 108 cm− 3), a higher resistivity (1.61 × 1010 Ω cm), and a larger ion migration activation energy (202.07 meV), decreasing the leakage current and baseline drift. The X-ray detector based on the EFG-CsPbBr3 SCs hence exhibits outstanding balanced performance, with a negligible dark current drift of 1.68 × 10− 9µA cm− 1 s− 1 V− 1, an incredibly low detection limit of 10.81 nGyair s− 1, and a sensitivity of 46180 µC Gyair−1 cm− 2 under a high electric field of 5000 V cm− 1. Furthermore, the detector maintains a stable response for 30 days and exhibits X-ray images for 70 and 120 keV irradiation. Our work provides an effective strategy to improve lead halide perovskite SCs for high-contrast X-ray detection and imaging.
Anti-Rho GTPase-activating protein 26 autoantibody-associated autoimmune cerebellar ataxia with G4 medulloblastoma: A case report and literature review
Authors: Li Biyun , Xiao Huimei , Luo Xingang , Liu Fang , Wang Lingan , Pei Zheng , Chang Yanqun 
Published: May 2023

Abstract
Background
The Rho GTPase-activating protein 26 (ARHGAP26) antibody, or anti-Ca, was recently identified as a subacute cerebellar ataxia autoantibody. Medulloblastoma is the most malignant brain tumor in children, with G4 medulloblastoma being the most common subgroup. Ten cases involving ARHGAP26 autoantibody-associated autoimmune cerebellar ataxia (ACA) have been reported to date, with an age of onset of 24 to 84 years. Five patients had a history of tumors. However, there is no reported case of ARHGAP26 ACA coexisting with G4 medulloblastoma.
Case presentation:
Here we report a 28-month-old boy with cerebellar ataxia associated with the presence of anti-ARHGAP26 autoantibody and G4 medulloblastoma. The patient demonstrated gait instability, coughing when drinking, and irritability and was positive for ARHGAP26 antibodies in the serum. Magnetic resonance imaging showed a space-occupying lesion in the fourth ventricle and supratentorial obstructive hydrocephalus. A pathological section of the lesion revealed G4 medulloblastoma originating from the cerebellar vermis.
Conclusions
This case may further the understanding of anti-ARHGAP26 autoantibody-associated cerebellar ataxia, its age of onset, and associated tumors. This case also suggests a connection between ARHGAP26 antibodies and G4 medulloblastoma. Therefore, we recommend anti-ARHGAP26 antibody testing for patients diagnosed with ACA. Moreover, we propose that simultaneous cerebrospinal fluid (CSF) and serum testing may help elucidate the connection between the presence of a tumor and anti-ARHGAP26 antibodies in the CSF or serum samples. However, in some cases, testing only serum samples may be sufficient for the diagnosis. Although, double sample tests are necessary to explore pathogenic relevance.
The effect of long-term irrigation with treated wastewater on some chemical properties of soil layers in (Kermanshah city Located in the west of Iran)
Authors: Ghamarnia Houshang , Farmanifard Milad , Palash Meisam 
Published: May 2023

Abstract

In different countries located in arid and semi-arid regions of the world, one of the most important problems in the agricultural sector is finding different alternative and reliable water sources for irrigation and wastewater is the only source of water that is increasing despite the decrease in the volume of other sources. Therefore, this study was performed to investigate the effects of long-term irrigation with municipal treated wastewater (TWW) of Kermanshah city on cover lands compared to well water (WW) as a control treatment on soil chemical properties in three layers with three replications in a two-year split plot trial design. The results showed that irrigation with TWW compared to WW caused an increase of 35.94, 32.35, 38.24, 20, 76, 1.68, 40, 40.77, 4.27 percent in the Cl-, HCO3-, CaCO3, TN, TP, K, OC, EC, pH in the 0–90 cm soil layer respectively. According to these results, the increasing of soil organic carbon, in addition to soil fertility, can stabilize and stop the mobility of heavy metals by creating organic bonds with heavy metals. Also, the results showed that due to sufficient access to treated wastewater; it is possible to do leaching, which reduces the risk of salinization of those lands. However, in some areas, increase of sodium percentage led the soil compaction and permeability reduce of lands due to the high clay content.
The relationship between exercise identity and exercise behavior among Chinese college students: the moderating role of weight bias.
Authors: Ren yilin , Zhu fengshu 
Published: May 2023

Abstract
Objective: Based on the deviance regulation theory, this paper aimed to explore the relationship between exercise identity, and adaptive and maladaptive exercise behavior, as well as its underlying mechanism among Chinese college students.
Method: A total of 1300 college students (mean aged: 18.75±3.2, 50% male) were investigated with Exercise Identity Scale, Physical Activity Rating Scale, Exercise Dependence Scale, and Weight Bias Questionnaire. SPSS27.0 and Mplus8.3 were used to conduct the statistical analysis.
Results: There were significant differences in adaptive and maladaptive exercise behaviors among college students of different genders (t=14.66, p =0.00; t=12.55, p=0.00).
Results: There were significant differences in adaptive and maladaptive exercise behaviors among college students of different genders (F=12.11, p=0.00; F=3.80, p =0.02; F=22.53, p =0.00; F=11.04, p=0.00). Among college students, ' exercise identity, weight bias and adaptive exercise behavior (r=0.50, p＜0.01; r=0.33, p＜0.01) and its dimensions, and maladaptive exercise behavior (r=0.51, p＜0.01; r=0.36, p＜0.01)and its dimensions were significantly positively correlated. Weight bias contributed to a positive moderating role in college students' exercise identity and adaptive exercise behaviors, as well as in maladaptive exercise behaviors.
Conclusion: Exercise identity can positively predict adaptive and maladaptive exercise behaviors among Chinese college students, in which weight bias contributes to a positive moderating role.
Early colonization of the intestinal microbiome of neonatal piglets is influenced by the maternal microbiome
Authors: Lim Jin A , Cha Jihye , Jung Seul a , Kim Nayeon , Song Min-Ji , Lim Young-Jo , Kim Dahye 
Published: May 2023

Abstract
The intestinal microbiome plays a crucial role in animal health and growth by interacting with the host, inhibiting pathogenic microbial colonization and regulating immunity. This study investigated dynamic changes in the fecal microbial composition of piglets from birth to weaning and the relationship between piglet fecal microbiome and sows. Feces, skin, amniotic fluid, and vaginal samples were collected from eight sows and sixty-three piglets, and the 16S rRNA gene was sequenced. The results revealed that the Firmicutes, Bacteroidetes, and Proteobacteria dominated the piglets’ microbiome in their early stages, and Firmicutes and Bacteroidetes were crucial to maintaining a balance in the intestinal microbiome during nursing. Abundances of Christensenellaceae_R-7_group, Succinivibrio, and Prevotella increased in weaned piglets fed solid feed. Analysis of the microbiome from sows to piglets indicated a shift in the microbiome colonizing piglet intestines and became a significant constituent of the piglet intestinal microbiome. This study supports the theory that the neonatal intestinal microbiome is vertically transmitted from the mother. Further research is needed to integrate sows, piglets, and their environments to gain a better understanding of the early establishment of the piglet intestinal microbiome.
Dysfunction of External Switching and Backward Inhibition in Major Depressive Disorder：General or Emotional specific?
Authors: Huang Sai , Zhou Jing , Zhao Yating , Lu Xiaobing , Zhang Bao 
Published: May 2023

Abstract

It remains controversial whether individuals with major depressive disorder (MDD) have dysfunctions in switching and/or inhibition when shifting their attention from one task set to another and whether such dysfunctions, if present, are general or specific to emotional content. In the present study, we investigated these questions with a modified task-switching task to measure the switch cost (the ability to activate a new set) and backward inhibition (the ability to inhibit a task set no longer relevant) in both clinical MDD patients and non-depressive control (NDC) individuals. The dysfunctions were identified to be in the emotional domain. Compared with NDCs, MDDs showed general and emotion-specific dysfunctions in external switching, especially difficulty switching attention from an emotional task to a non-emotional task. Further, this dysfunction was independent of rumination. In contrast, although emotional dysfunction in backward inhibition was also observed in MDD, it was closely related to elevated rumination scores. The results suggest that different symptoms of MDD were associated with different cognitive dysfunctions.
Title: Income inequality, unemployment, and government transfer: what does their dynamics tell us
Authors: Ahmed Haydory A. , Shadmani Hedieh 
Published: May 2023

Abstract
In this research, we explore the dynamics among measures of income inequality in the United States, male and female unemployment rates, and growth in government transfer using time series data. This research adopts a macro-econometric approach using a structural VAR model. Our structural impulse responses find growth in government transfer increases unemployment rates for both males and females. Female income inequality declines with increased government transfer. When the female income ratio rises, we observe the government transfer outlays fall over the forecast horizon. Variance decomposition finds that growth in government transfers is impacted by the male unemployment rate relatively more than the female unemployment rate. This research, therefore, suggests gender-specific government transfers to reduce income inequality. This, in effect, may reduce government transfer outlays over time.
JEL code: C32, D63, E24, I38, J16
Transient, accelerating slow slips drive the Preparatory Process of the 2023 Mw 7.8 Türkiye Earthquake
Authors: Picozzi Matteo , Iaccarino Antonio Giovanni 
Published: May 2023

Abstract

On 6 February 2023, Southern Türkiye was struck by a magnitude 7.8 earthquake. Especially during major events, seismologists are confronted with the inability to predict earthquakes. Indeed, how, when, and where large earthquakes are generated remain fundamental unsolved scientific questions. We analyze the spatio-temporal evolution of thousands of small magnitude earthquakes for the two years that preceded the mainshock aiming to highlight a preparatory process existence. We find patterns in seismic spatio-temporal distribution suggesting the existence of transient slow slip episodes accelerating in the fifteen months preceding the mainshock. Slow slip seems responsible for a progressive decoupling process in the main event nucleation area, which is highlighted by the fraction of non-clustered seismicity increase. The latter preparation phase started ~seven months before the 9 February 2023, Southern Türkiye Mw 7.8 earthquake. Our observations stimulate significant questions about East Anatolian Fault mechanics. Intercepting when a fault system starts deviating from its steady behavior, might be the key for identifying the preparatory phase of large earthquakes.