bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative analysis of the risk of hydrogen sulfide release from gas hydrates
Authors: Wang Xianqing , Liu Siqing , Zhao Bin , Yao Yanfu , Wu Gang , Xie Rui , Fu Yutong , Ning Zijie 
Published: January 2023

The role that H2S plays in the global sulfur cycle has been studied extensively in recent years. This paper focuses on the influence of H2S released from gas hydrates on sulfur cycle and establishes a one-dimensional mathematical model to calculate the amount of H2S released from the dissociation of gas hydrates present in multiple layers in the Qiongdongnan Basin China. The results show that the sulfate and methane transition zone that covers an area of about 100 km2in the Qiongdongnan Basin contains 2.3 × 1012 g of pyrite, which requires 4.06 × 1011 mol of H2S for its formation. The H2S released from the dissociation of gas hydrates is 5.4 ×1011 mol, which is about 1.3 times that needed for the formation of pyrite. Therefore, the H2S released from the gas hydrates is an important source of H2S for the formation of pyrite in the sulfate-methane transition zone of Qiongdongnan Basin. According to the flux of H2S and the partial pressure of O2 (PO2) in the atmosphere, the critical value of the balance between the flux of H2S and PO2 turns out to be 0.13 mol kg−1∙bar−1. Furthermore, considering the effect of global sea-level changes, three risk modes are identified to categorize the amount of H2S released from the dissociation of gas hydrate into the atmosphere. We classify the periods from 5–12 Ma BP, 25–29 Ma BP, 47–52 Ma, and 57–61 Ma BP into the high-risk mode. Furthermore, the results show that a part of the H2S released from the gas hydrate dissociation is oxidized by the Fe (III) oxide metal, with much of the metal ions being released into the pore water. Another part of the H2S is re-oxidized by the O2 in the ocean, with much of SO42- released into the seawater. Therefore, the process also provides metal ions and SO42- to pore water or seawater when the H2S released from gas hydrate diffuses from the bottom. This paper provides new insights into the source of H2S in the ocean and shows that the H2S contained in gas hydrates plays an important role in the global sulfur cycle.
Crustal velocity structure in the South Yellow Sea revealed by the joint tomographic inversion of reflected and refracted seismic waves
Authors: Ma Fei , Hou Fanghui , Li Tongyu , Wu Zhiqiang , Zhang Jianzhong 
Published: January 2023

The crustal velocity structure in the South Yellow Sea (SYS) Basin is crucial for understanding the basin’s geological structure and evolution. OBS (ocean-bottom station) data from the OBS2013 line have been used to determine the crustal velocity structure in the SYS. The velocity model of the upper crust in the northern SYS was determined using first-arrival traveltime tomography. The model showed a higher resolution shallow crustal velocity structure but a lower resolution middle-lower crustal velocity structure. The crustal velocity structure, together with the Moho discontinuity in the SYS Basin, was also constructed using a human–computer interactive traveltime simulation, and the result was highly dependent on the prior knowledge of the operator. In this study, we reconstructed a crustal velocity model in the SYS Basin using a joint tomographic inversion of the traveltime and its gradient data of the reflected and refracted waves picked from the OBS data. The resolution of the inverted velocity structure from shallow-to-deep crust was improved. The results revealed that the massive high-velocity body below the Haiyang Sag of the Jiaolai Basin extends to the Qianliyan Uplift in the SYS; the low-velocity Cretaceous strata directly cover the pre-Sinitic metamorphic rock basement of the Sulu orogenic belt; and the thick Meso-Paleozoic marine strata are retained beneath the Meso–Cenozoic continental strata in the northern depression. The Moho depth in the SYS Basin ranges from 28 to 32 km.
Structural boundary and deep contact relationship between the Yangtze and Cathaysia Blocks from crustal thickness gradients
Authors: Han Rubing , Yang Dinghui , Li Qiusheng , Fu Wei , Zhu Gaohua , Zhang Hongshuang , Chen Hao , Cheng Yongzhi 
Published: January 2023

The deep boundary and contact relationship between the Yangtze and Cathaysia Blocks (the major tectonic units of the Southern China Block), as well as the tectonic attributes of the Jiangnan Orogenic Belt, have remained unknown or controversial. Using data recorded by 128 portable broadband stations and 96 permanent stations, we obtained high-resolution images of crustal thickness and Poisson’s ratio in the study area. The influences of crustal anisotropy and inclined interface were eliminated by using the newly proposed receiver function H–κ–c stacking method. We then used a gradient analysis method to obtain crustal thickness gradients at the boundary of the Yangtze and Cathaysia Blocks for the first time. Our results reveal that the crustal thickness varies from &gt;38 km in the Qinling–Dabie Orogenic Belt to &lt;30 km east of the Tanlu Fault and Cathaysia Block. Areas with high Poisson’s ratios (&gt;0.27) are concentrated on the flanks of the deep fault zone and the continental margin of the study area; those with low Poisson’s ratios (&lt;0.23) are concentrated in the Jiangnan Orogenic Belt. Large crustal thickness gradients are found beneath the eastern part of the Jiujiang–Shitai buried fault (&gt;5 km/°). Combined with the velocity structure and discontinuity characteristics at different depths, these findings suggest that the Jiujiang–Shitai fault may constitute a deep tectonic boundary dividing the Yangtze and Cathaysia Blocks on the lithospheric scale. Moreover, our results support that the Cathaysia Block subducted northwest-ward toward the southeastern margin of the Yangtze Block in the Neoproterozoic, and that the Jiujiang–Shitai buried fault and Jiangshan–Shaoxing fault are the deep and shallow crustal contact boundaries of the two Blocks, respectively; that is, the Yangtze Block overlaps the Cathaysia Block.
Control mechanism of the migration of heavy metal ions from gangue backfill bodies in mined-out areas
Authors: Zhang Yun , Liu Yongzi , Liu Jia , Zhan Rui , He Wei , Tong Liang , Wan Peifeng , Bai Licheng 
Published: January 2023

In the process of solid backfill mining, the leaching of heavy metal ions from the gangue backfill body in the mined-out area can pose potential risk of polluting water resources in the mine. Accordingly, based on the environment of the gangue backfill body, the migration model of heavy metal ions from the gangue backfill body was established to reveal the pollution mechanism of water resources by the gangue backfill body in the mined-out area. The main factors that affect the migration of heavy metal ions were analyzed, and prevention and control techniques for the leaching and migration of heavy metal ions from gangue backfill bodies were proposed. Research showed that the heavy metal ions in gangue backfill bodies were subjected to the coupled action of seepage, concentration, and stress and then driven by water head pressure and gravitational potential energy to migrate downward along the pore channels in the floor, during which mine water served as the carrier. The migration distance of heavy metal ions increased with time. According to the migration rate, the migration process can be subdivided into three phases: the rapid migration phase (0–50 years), the slow migration phase (50–125 years), and the stable phase (125–200 years). It was concluded that the leaching concentration of heavy metal ions, the particle size of gangue, the permeability of floor strata, and the burial depth of coal seams were the main influencing factors of the migration of heavy metal ions. From the two perspectives of heavy metal ion leaching and migration, prevention and control techniques for the leaching and migration of heavy metal ions from gangue backfill bodies were proposed to protect water resources in mining area. The present study is of great significance to realizing utilization of solid waste in mines and protecting the ecological environment.
Real world effectiveness of subcutaneous semaglutide in type 2 diabetes: A retrospective, cohort study (Sema-MiDiab01)
Authors: Berra Cesare C. , Rossi Maria Chiara , Mirani Marco , Ceccarelli Ceccarelli Daniela , Romano Cristina , Sassi Lorenza , Peretti Elena , Favacchio Giuseppe , Pastore Ida , Folini Laura , Graziano Giusi , Lunati Maria Elena , Solerte Sebastiano Bruno , Fiorina Paolo 
Published: January 2023

IntroductionAim of the present study was to evaluate the real-world impact of once-weekly (OW) subcutaneous semaglutide on different end-points indicative of metabolic control, cardiovascular risk factors, and beta-cell function in type 2 diabetes (T2D).MethodsThis was a retrospective, observational study conducted in 5 diabetes clinics in Italy. Changes in HbA1c, fasting blood glucose (FBG), body weight, blood pressure, lipid profile, renal function, and beta-cell function (HOMA-B) during 12 months were evaluated.ResultsOverall, 594 patients (97% GLP-1RA naïve) were identified (mean age 63.9 ± 9.5 years, 58.7% men, diabetes duration 11.4 ± 8.0 years). After 6 months of treatment with OW semaglutide, HbA1c levels were reduced by 0.90%, FBG by 26 mg/dl, and body weight by 3.43 kg. Systolic blood pressure, total and LDL-cholesterol significantly improved. Benefits were sustained at 12 months. Renal safety was documented. HOMA-B increased from 40.2% to 57.8% after 6 months (p&lt;0.0001).DiscussionThe study highlighted benefits of semaglutide on metabolic control, multiple CV risk factors, and renal safety in the real-world. Semaglutide seems to be an advisable option for preservation of β-cell function and early evidence suggests it might have a role in modifying insulin resistance (HOMA-IR), the pathogenetic basis of prediabetes and T2D.
Benefits of Huang Lian mediated by gut microbiota on HFD/STZ-induced type 2 diabetes mellitus in mice
Authors: Li Dan , Feng Guangli , Li Yue , Pan Han , Luo Pei , Liu Bo , Ding Tao , Wang Xin , Xu Huibo , Zhao Yufeng , Zhang Chenhong 
Published: January 2023

BackgroundHuang Lian (HL), one of the traditional Chinese medicines (TCMs) that contains multiple active components including berberine (BBR), has been used to treat symptoms associated with diabetes for thousands of years. Compared to the monomer of BBR, HL exerts a better glucose-lowering activity and plays different roles in regulating gut microbiota. However, it remains unclear what role the gut microbiota plays in the anti-diabetic activity of HL.MethodsIn this study, a type 2 diabetes mellitus (T2DM) mouse model was induced with a six-week high-fat diet (HFD) and a one-time injection of streptozotocin (STZ, 75 mg/kg). One group of these mice was administrated HL (50 mg/kg) through oral gavage two weeks after HFD feeding commenced and continued for four weeks; the other mice were given distilled water as disease control. Comprehensive analyses of physiological indices related to glycolipid metabolism, gut microbiota, untargeted metabolome, and hepatic genes expression, function prediction by PICRUSt2 were performed to identify potential mechanism.ResultsWe found that HL, in addition to decreasing body fat accumulation, effectively improved insulin resistance by stimulating the hepatic insulin-mediated signaling pathway. In comparison with the control group, HL treatment constructed a distinct gut microbiota and bile acid (BA) profile. The HL-treated microbiota was dominated by bacteria belonging to Bacteroides and the Clostridium innocuum group, which were associated with BA metabolism. Based on the correlation analysis, the altered BAs were closely correlated with the improvement of T2DM-related markers.ConclusionThese results indicated that the anti-diabetic activity of HL was achieved, at least partly, by regulating the structure of the gut microbiota and the composition of BAs.
RNA modification writers pattern in relation to tumor microenvironment and prognosis in prostate cancer
Authors: Cheng Xu , Yi Xuanzi 
Published: January 2023

Background: RNA modifications are important in the study of epigenetic regulatory mechanisms in immune responses and tumorigenesis. When RNA writers are mutated or disrupted in expression, the genes associated with the pathways they modify are also disrupted and can activate or repress related pathways, affecting tumorigenesis and progression. However, the potential role of RNA writers in prostate cancer is unclear.Methods: Based on data from three datasets, we describe 26 RNA writers that mediate gene expression and genetic mutation in prostate cancer and assess their expression patterns in 948 prostate cancer samples. Using principal component analysis algorithms, the RM Score was developed to quantify the RNA modification patterns of specific tumors.Results: Two different categories were determined by unsupervised clustering methods, and survival analysis showed significant differences in OS prognosis between these two categories. Differentially expressed genes between the different categories were detected and the RNA writers-mediated scoring model RM_Score were constructed based on this. Also, the RM_Score was analyzed in relation to clinical characteristics, immune infiltration level, drug response, and efficacy of chemotherapy and immunotherapy. Those results confirm that multilayer alterations in epitope-modified RNA writers are associated with patient prognosis and with immune cell infiltration characteristics. Finally, we examined differentially expressed mRNA, lncRNA and miRNA between high and low RM_Score groups, based on which a ceRNA regulatory network was constructed.Conclusion: This work is a comprehensive analysis of modified writers in prostate cancer and identified them to have a role in chemotherapy and immunotherapy.
Modeling C3 glomerulopathies: C3 convertase regulation on an extracellular matrix surface
Authors: Pisarenka Sofiya , Meyer Nicole C. , Xiao Xue , Goodfellow Renee , Nester Carla M. , Zhang Yuzhou , Smith Richard J. H. 
Published: January 2023

IntroductionC3 glomerulopathies (C3G) are ultra-rare complement-mediated diseases that lead to end-stage renal disease (ESRD) within 10 years of diagnosis in ~50% of patients. Overactivation of the alternative pathway (AP) of complement in the fluid phase and on the surface of the glomerular endothelial glycomatrix is the underlying cause of C3G. Although there are animal models for C3G that focus on genetic drivers of disease, in vivo studies of the impact of acquired drivers are not yet possible.MethodsHere we present an in vitro model of AP activation and regulation on a glycomatrix surface. We use an extracellular matrix substitute (MaxGel) as a base upon which we reconstitute AP C3 convertase. We validated this method using properdin and Factor H (FH) and then assessed the effects of genetic and acquired drivers of C3G on C3 convertase.ResultsWe show that C3 convertase readily forms on MaxGel and that this formation was positively regulated by properdin and negatively regulated by FH. Additionally, Factor B (FB) and FH mutants impaired complement regulation when compared to wild type counterparts. We also show the effects of C3 nephritic factors (C3Nefs) on convertase stability over time and provide evidence for a novel mechanism of C3Nef-mediated C3G pathogenesis.DiscussionWe conclude that this ECM-based model of C3G offers a replicable method by which to evaluate the variable activity of the complement system in C3G, thereby offering an improved understanding of the different factors driving this disease process.
Five genes as diagnostic biomarkers of dermatomyositis and their correlation with immune cell infiltration
Authors: Zhao Xiaohu , Si Shangkun 
Published: January 2023

BackgroundDermatomyositis (DM) is a rare autoimmune disease characterized by severe muscle dysfunction, and the immune response of the muscles plays an important role in the development of DM. Currently, the diagnosis of DM relies on symptoms, physical examination, and biopsy techniques. Therefore, we used machine learning algorithm to screen key genes, and constructed and verified a diagnostic model composed of 5 key genes. In terms of immunity, The relationship between 5 genes and immune cell infiltration in muscle samples was analyzed. These diagnostic and immune-cell-related genes may contribute to the diagnosis and treatment of DM.MethodsGSE5370 and GSE128470 datasets were utilised from the Gene Expression Omnibus database as DM test sets. And we also used R software to merge two datasets and to analyze the results of differentially expressed genes (DEGs) and functional correlation analysis. Then, we could detect diagnostic genes adopting least absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine recursive feature elimination (SVM-RFE) analyses. The validity of putative biomarkers was assessed using the GSE1551 dataset, and we confirmed the area under the receiver operating characteristic curve (AUC) values. Finally, CIBERSORT was used to evaluate immune cell infiltration in DM muscles and the correlations between disease-related biomarkers and immune cells.ResultsIn this study, a total of 414 DEGs were screened. ISG15, TNFRSF1A, GUSBP11, SERPINB1 and PTMA were identified as potential DM diagnostic biomarkers(AUC &gt; 0.85),and the expressions of 5 genes in DM group were higher than that in healthy group (p &lt; 0.05). Immune cell infiltration analyses indicated that identified DM diagnostic biomarkers may be associated with M1 macrophages, activated NK cells, Tfh cells, resting NK cells and Treg cells.ConclusionThe study identified that ISG15, TNFRSF1A, GUSBP11, SERPINB1 and PTMA as potential diagnostic biomarkers of DM and these genes were closely correlated with immune cell infiltration.This will contribute to future studies in diagnosis and treatment of DM.
Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach
Authors: Zhou Li , Li Mengna , Zhong Zhaoshan , Chen Hao , Wang Minxiao , Lian Chao , Wang Hao , Zhang Huan , Cao Lei , Li Chaolun 
Published: January 2023

IntroductionMarine metal contamination caused by deep-sea mining activities has elicited great concern from both social and scientific communities. Among the various metals deep-sea organisms might encounter, cadmium (Cd) is a widely detected metal that in very small amounts is nonetheless capable of severe toxicity. Yet due to both remoteness and technical challenges, insights into the effects of metal exposure resulting from mining activities upon deep-sea organisms are limited.MethodsHere, we investigated Cd’s toxicological effects on deep-sea mussels of Gigantidas platifrons exposed to 100 or 1000 g/L of Cd for 7 days; an integrated approach was used that incorporated proteomics and metabolomics along with traditional approaches (metal concentrations, metal subcellular distribution, and anti-oxidative and immune-related biochemical indexes).Results and DiscussionResults showed that Cd exposure caused significant Cd’s accumulation in mussel gills and redistribution of Cd among subcellular compartments, with cellular debris being the primary binding site. Although anti-oxidative enzymes activities (superoxide dismutase and catalase) were not significantly altered in mussel gills of both exposed groups, the markedly increased level of glutathione S-transferase detected via proteomic technique clearly evinced that deep-sea mussels suffered from oxidative stress under Cd exposure. Besides, altered activities of acid phosphatase and alkaline phosphatase assayed by traditional methods along with the predominant presence of largely altered immune-related proteins detected by proteomic data strongly revealed an immune response of deep-sea mussels elicited by Cd. In addition, results of proteomics combined with those of non-targeted metabolomics demonstrated that Cd could exert toxicity by disrupting cytoskeleton structure, ion homeostasis, and primary metabolisms of energy, lipid, and nucleotide in deep-sea mussels. As demonstrated in this study, proteomics and metabolomics can be used in tandem to provide valuable insights into the molecular mechanisms of deep-sea organisms’ response to Cd exposure and for helping to discover potential biomarkers for application during deep-sea mining assessments.
Retinal detachments in a patient with minimal change nephrotic syndrome: Case report and review of the literature
Authors: Ye Chunjian , Qiu Miaohua , Zhong Yu , Chen Jiejian 
Published: January 2023

BackgroundTo report an unusual case of minimal change nephrotic syndrome with sudden bilateral retinal detachment.Case presentationA 54-year-old woman with minimal change nephrotic syndrome presented with sudden-onset visual blurring in both eyes. Optical coherence tomography scans revealed macular schisis and extramacular intraretinal separation. A kidney biopsy confirmed the diagnosis of minimal change disease. Glucocorticoid therapy was quickly started. During remission, her vision was restored, with complete resolution of the subretinal fluid observed on optical coherence tomography.ConclusionsIn minimal change nephrotic syndrome, fluid accumulation in the retina layer may occur, and gravity-induced vitreous traction on the inferior retina may cause retinal detachment. Patients should be advised to avoid large swings of the head and neck, handstands, and other activities that may increase the risk of retinal detachment. The possibility of retinal detachment should be considered when blurred vision occurs.
Advanced age is associated with increased adverse outcomes in patients undergoing middle cerebral artery stenting
Authors: Wang Gong , Liu Juan , He Shengrong , Zhang Xi , Yang Libai , Gao Fei , Guo Yu , Xu Rui 
Published: January 2023

PurposeThis study tried to evaluate whether advanced age has an increased incidence of major complications in patients undergoing MCA stenting.MethodsA total of 348 patients who underwent MCA stenting were reviewed from a prospectively maintained database. Ninety-day ischemic stroke, intracerebral hemorrhage, and death outcomes were compared among the young (≤40 years old), middle (41–60 years old) and old (≥61 years old) groups. Univariate analysis and multivariable logistic regression analysis were used to investigate different variables associated with 90-day major adverse events. Kaplan–Meier analysis was performed to determine long-term outcomes during follow-up.ResultsThe incidence of 90-day ischemic stroke was 9.26% in the old group, 2.86% in the middle group, and 0% in the young group (P = 0.024). The incidence of all 90-day major adverse events was 3.33% in patients ≤40 years old, 19.90% in patients 41–60 years old, and 24.07% in patients ≥61 years old, with statistical significance (P = 0.04). Advanced age was associated with increased 90-day ischemic stroke (OR = 1.074, 95% CI: 1.019–1.132, P = 0.007; adjusted OR: 1.071, 95% CI: 1.008–1.138, P = 0.026) and 90-day death (OR = 1.072, 95% CI: 1.012–1.135, P = 0.018; adjusted OR: 1.095, 95% CI: 1.015–1.182, P = 0.018). Meanwhile, advanced age was also associated with decreased long-term survival and ischemic stroke-free survival during follow-up.ConclusionOur data indicated that MCA stenting in elderly patients is associated with a high risk of adverse events and should be cautiously considered.