bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opposite response of N2O emissions in different seasons to warming and precipitation addition on a temperate steppe
Authors: Wan Zhiqiang , Gu Rui , Ganjurjav Hasbagan , Hu Guozheng , Gao Qingzhu , Chen Xuemeng , Gu Xiling , Chun Xi , Zhou Haijun 
Published: June 2023

AbstractClimate change is an important issue that affects both global warming and precipitation, and the main cause is increased N2O emissions. Temperature and moisture are key factors in grassland ecosystem's response to climate change, and they can affect N2O fluxes. To clarify the impacts of warming and precipitation changes on N2O fluxes, an experiment was conducted in a semiarid steppe in Inner Mongolia, China over a nine‐year period (2011–2019). Plant productivity and soil nutrient dynamics were examined concurrently from 2017 to 2019, and N2O fluxes were monitored in response to different treatment conditions: control (C), warming (W), precipitation addition (P), and warming and precipitation addition (WP). The results showed that N2O emissions in the growing season were higher than those in the nongrowing season. Warming and precipitation addition had no significant effect on N2O fluxes compared with ambient conditions. Compared with P treatment, warming increased N2O flux in nongrowing season and decreased it in growing season. N2O flux was positively correlated with soil temperature and moisture (p < 0.05). Warming had a significant positive effect on soil NH4+‐N, whereas additional precipitation had a large positive effect on soil total nitrogen and soil nitrate nitrogen. With the WP treatment, soil microbial biomass nitrogen (MBN) and soil microbial biomass carbon (MBC) increased by 53.8% and 41.9%, respectively. The decrease in N2O emissions during the growing season in the W treatment compared with the P treatment may be largely attributed to the greater dominance of Leymus chinensis. The results highlight that changes in species dominance play an important role in regulating N2O emissions, and that the N2O fluxes in the nongrowing season account for a large proportion of the changes in N2O fluxes. Therefore, The warming effects on N2O emissions during nongrowing seasons should be further investigated.
The prognostic impact of specialist cardiology input in patients admitted for heart failure and normal ejection fraction
Authors: Cannata Antonio , Badawy Layla , Anyu Anawinla Ta , Samways Jack , Sweeney Mark , Jordan‐Rios Antonio , Zakeri Rosita , Scott Paul A. , Piper Susan , Plymen Carla M. , McDonagh Theresa A. , Bromage Daniel I. 
Published: June 2023

AbstractAimsSpecialist cardiology care is associated with a prognostic benefit in patients with heart failure (HF) with reduced ejection fraction (HFrEF) admitted with decompensated HF. However, up to one third of patients admitted with HF and normal ejection fraction (HFnEF) do not receive specialist cardiology input. Whether this has prognostic implications is unknown.Methods and resultsData on patients hospitalized with HFnEF from two tertiary centres were analysed. The primary outcome measure was all‐cause mortality during follow‐up. The secondary outcome was in‐hospital mortality. A total of 1413 patients were included in the study. Of these, 23% (n = 322) did not receive in‐hospital specialist cardiology input. Patients seen by a cardiologist were less likely to have hypertension (73% vs. 79%, P = 0.03) and respiratory co‐morbidities (25% vs. 31%, P = 0.02) compared with those who did not receive specialist input. Similarly, clinical presentation was more severe for those who received specialist input (New York Heart Association III/IV 83% vs. 75% respectively, P = 0.003; moderate‐to‐severe peripheral oedema 65% vs. 54%, P < 0.001). Medical management was similar, except for a higher use of diuretics (90% vs. 86%, P = 0.04) and a longer length of stay for patients who received specialist input (9 vs. 4 days, P < 0.001). Long‐term outcomes were comparable between patients who received specialist input and those who did not. However, specialist input was independently associated with lower in‐hospital mortality (hazard ratio 0.19, confidence interval 0.09–0.43, P < 0.001).ConclusionsIn‐hospital cardiology specialist input has no long‐term prognostic advantage in patients with HFnEF but is independently associated with reduced in‐hospital mortality.
Circ_0000033 up‐regulates NUAK2 by sequestering miR‐378a‐3p to promote breast tumorigenesis
Authors: Dai Yijun , Shi Wenjian , Qiu Yanru , Xu Tianwen , Lin Jianguang , Su Yunxia 
Published: June 2023

AbstractCircular RNAs (circRNAs), including circ_0000033, were shown to be abnormally expressed in breast cancer (BC) and play an important regulatory function in the development of this cancer. This study aimed to investigate the action and mechanism of circ_0000033 in BC carcinogenesis. Specifically, levels of genes and proteins were analyzed using quantitative real‐time PCR (qRT‐PCR) and western blotting. Circ_0000033 was highly expressed in BC tissues and cells. Properties of cells with modified expression of circ_0000033 were characterized using an in vitro colony formation assay, EdU assay, flow cytometry, caspase‐3 activity analysis, transwell assay, and tube formation assay, respectively. Functionally, knockdown of circ_0000033 suppressed BC cell proliferation, migration, invasion, angiogenesis, and induced apoptosis and cell cycle arrest in vitro. An in vivo experiment was conducted using a murine xenograft model and showed circ_0000033 silencing also impeded the growth of BC in nude mice. The binding between miR‐378a‐3p and circ_0000033 or NUAK2 (NUAK Family Kinase 2) was validated using a dual‐luciferase reporter assay. Circ_0000033 sequestered miR‐378a‐3p and resulted in NUAK2 release, indicating a circ_0000033/miR‐378a‐3p/NUAK2 regulatory network operates in BC cells. Circ_0000033 down‐regulation in BC cells was accompanied by decreased NUAK2 and increased miR‐378a‐3p expression. Moreover, the anticancer effects mediated by circ_0000033 knockdown were abolished by miR‐378a‐3p inhibition or NUAK2 overexpression in BC cells. Overall, circ_0000033 up‐regulates NUAK2 through sequestration miR‐378a‐3p, which promoted breast tumorigenesis, suggesting circ_0000033 is a promising therapeutic target for BC treatment.
Solid oxide cell electrolytes deposited by atmospheric suspension plasma spraying at high velocity and high temperature
Authors: Kuhn Joel , Kesler Olivera 
Published: June 2023

AbstractMetal‐supported solid oxide cells with Yttria‐stabilized zirconia (YSZ) electrolytes fabricated by atmospheric plasma spraying are routinely found to have open‐circuit voltages (OCVs) below the Nernst potential due to gas crossover and combustion resulting from electrolyte defects. To improve splat bonding and reduce coating defects, YSZ electrolytes were fabricated here at >800°C substrate temperatures and torch‐substrate relative velocities of 4 and 12 m/s by atmospheric suspension plasma spraying. Electrolyte microstructures appeared dense, with porosities estimated to be approximately 2.2–3.5 vol%. Minimal segmentation cracking was observed on samples fabricated at 12 m/s. The full cells that were electrochemically tested had permeabilities in the range of 4–6 × 10−19 m2, and the maximum recorded OCV was ∼26 mV below the Nernst potential for 750°C. Potential performance gains from YSZ deposition at substrate temperatures >800°C may have been masked by poor substrate‐fuel electrode contact. Using electrochemical impedance spectroscopy, it was found that the ohmic and polarization resistances decreased and increased, respectively, over time. The calculated distribution of relaxation times of the tested cells, together with observations from the literature, were employed to identify possible cell degradation mechanisms observed during short‐term durability testing.
Benefits of nanotechnology for occupational ergonomic design: A positive step toward more protection of workers' health
Authors: Nasirzadeh Nafiseh , Omari Shekaftik Soqrat , Keshavarz Zahra 
Published: June 2023

AbstractNowadays, the combination of “Nanotechnology” and “Ergonomics” has been known as “Nano‐ergonomics.” Nano‐ergonomics can help to develop more comfortable conditions in workplaces. So, the aim of this study is to reveal benefits of nanotechnology for occupational health and safety design, especially ergonomic design. The search strategy was provided based on cochrane guidelines with main search terms of “Nanotechnology,” “Nanomaterial,” and “Nanoparticle” combined with “Ergonomics” and “Human Factors.” PubMed, Scopus, Web of Sciences and Google Scholar Databases were researched for relevant articles. Also, Google search engine was used to find nano‐ergonomic commercial products and to complete the research with identifying additional information. A total of 32 articles were first achieved. By providing Synthesis Without Meta‐analysis reporting guideline, finally, four studies were regarded as appropriate. The results showed that nanotechnology has developed in three major areas of ergonomics such as physical, environmental, and cognitive ergonomics, which is a positive step toward more protection of workers' health. Although, there are not any original article related to nanotechnology for ergonomic product design, they are offered as the commercial products by the largest companies such as Amazon. Also, workers at the nanotechnology‐related industries have the challenges of exposure to toxic nanomaterials. So, before the application of nanomaterials, we should have proper knowledge of nanomaterials‐caused toxic hazards and how to handle them.
Using continuous electrical conductivity measurements to derive major solute concentrations in karst systems
Authors: Richieri Beatrice , Bittner Daniel , Hartmann Andreas , Benettin Paolo , van Breukelen Boris M. , Labat David , Chiogna Gabriele 
Published: June 2023

AbstractHydrochemical data of karst springs provide valuable insights into the internal hydrodynamical functioning of karst systems and support model structure identification. However, the collection of high‐frequency time series of major solute species is limited by analysis costs. In this study, we develop a method to retrieve the individual solute concentration time series and their uncertainty at high temporal resolution for karst springs by using continuous observations of electrical conductivity () and low‐frequency ionic measurements. Due to the large ion content and non‐negligible concentrations of aqueous complexes in karst systems, the concentration of each solute species occurring as free ion and as part of aqueous complexes are computed separately. The concentration of species occurring as free ions are computed considering their contributions to the total , whereas the concentration of the species as part of complexes are obtained from speciation calculations. The pivotal role of the complexation processes for the reconstruction of solute concentration time series starting from the signal is investigated in two karstic catchments with different geologies and temporal resolution of the available hydrochemical datasets, that is the Kerschbaum dolostone system in Austria and the Baget limestone system in France. The results show that complexation processes are significant and should be considered for the estimation of the total solute concentration in case of SO4, Ca, Mg and HCO3. The signal of a karst spring can be used to interpolate and quantify the dynamics of those solutes characterized by large contribution (approximately >6%) to the total and low relative variability, that is HCO3, Ca and Mg. Moreover, the presented method can be used to estimate concentrations of solutes when applied to karst systems with stationary and hydrogeochemical homogeneous contributing area. On the contrary, the method is affected by large uncertainty in case of dynamic systems characterized by varying contributions of water from different geological areas. This study aims to contribute to the problem of hydrogeochemical data availability and to support future works on karst systems conceptualization.
Advances in probing single biomolecules: From DNA bases to glycans
Authors: Hou Baofei , Zhang Teng , Yang Huixia , Han Xu , Liu Liwei , Li Linfei , Grazioli Cesare , Wu Xu , Jiang Nan , Wang Yeliang 
Published: June 2023

AbstractImaging biomolecules in real space is crucial for gaining a comprehensive understanding of the properties and functions of biological systems at the most fundamental level. Among the various imaging techniques available for biomolecules and their assembled nanostructures, scanning probe microscopy (SPM) provides a powerful and nondestructive imaging option. SPM is unique in visualizing intrinsically disordered biomolecules at the nanometer scale (e.g., glycans). This review highlights recent achievements in studying biomolecules using SPM technique, focusing on DNA bases, amino acids, proteins, and glycans. The atomic‐level analysis of biomolecules made possible by SPM allows for a more accurate definition of the local structure–property relationship. High‐resolution SPM imaging of single biomolecules offers a new way to study basic processes of life at the molecular level.
Risk of epithelial ovarian tumors among women with polycystic ovary syndrome: A nationwide population‐based cohort study
Authors: Frandsen Clarissa L. B. , Svendsen Pernille F. , Nøhr Bugge , Viuff Jakob H. , Maltesen Thomas , Kjær Susanne K. , Jensen Allan 
Published: June 2023

AbstractAn association between polycystic ovary syndrome (PCOS) and epithelial ovarian tumors is biologically plausible as conditions inherent to PCOS such as excessive androgenic hormones, reproductive factors and obesity are also risk factors for these hormone‐sensitive tumors. However, previous studies have showed conflicting results and have various methodological limitations. This population‐based cohort study investigates the association between PCOS and epithelial ovarian tumors and includes all women born in Denmark between January 1, 1940 and December 31, 1993 (n = 1 719 304). PCOS diagnoses, ovarian cancer and borderline ovarian tumor diagnoses, covariates, migration and vital status were obtained from the Danish national registers. Adjusted cox proportional hazard regression models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CI) for epithelial ovarian cancer and for borderline ovarian tumors overall as well as for histological subtypes separately. During median 26 years of follow‐up we identified 6490 women with ovarian cancer and 2990 women with borderline ovarian tumors. Overall, we observed no marked associations between a diagnosis of PCOS and overall epithelial ovarian cancer or overall epithelial borderline ovarian tumors, irrespective of time since diagnosis. However, we found an increased risk of ovarian cancer among postmenopausal women with PCOS (HR 2.28 95% CI 1.02‐5.09) and an increased risk of serous borderline ovarian tumors (HR 2.34 95% CI 1.21‐4.53) in women with PCOS compared with women without PCOS. Importantly, low statistical precision is a crucial limitation of our study and in previous studies and larger studies with longer follow‐up are therefore warranted.
Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota
Authors: Tian Yufei , Wu Jiacai , Zheng Yili , Li Mengyu , Xu Xia , Chen Hongyuan , Rui Wen 
Published: June 2023

AbstractBACKGROUNDAstragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi. Honey‐processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product. Polysaccharides are their main active components.RESULTSAPS2a and HAPS2a were initially isolated from Astragulus and honey‐processed Astragulus. Both of them are highly branched acidic heteropolysaccharides containing ɑ‐configuration and β‐configuration glycosidic bonds. The molecular weight and the molecular dimension of HAPS2a decreased and the GalA contained in APS2a was converted to Gal in HAPS2a. The α‐configuration galactose residue 1,3,4‐α‐Galp in the backbone of APS2a was converted to the corresponding β‐configuration galactose residue 1,3,4‐β‐Galp in the backbone of HAPS2a and the uronic acid residue T‐α‐GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T‐α‐Galp in the side chain of HAPS2a. Bioactivity results showed that HAPS2a had better probiotic effects on Bacteroides ovatus, Bacteroides thetaiotaomicron, Bifidobacterium longum and Lactobacillus rhamnosus strains than APS2a. After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition. The contents of total short‐chain fatty acids (SCFAs) and other organic acids in HAPS2a group were higher than APS2a group.CONCLUSIONSTwo novel high‐molecular‐weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing. Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement. © 2023 Society of Chemical Industry.
Natural regeneration offers an effective way for large‐scale restoration of degraded lands in Tigray, Ethiopia
Authors: Araya Haileselassie Ghebremariam , Wasonga Oliver Vivian , Mureithi Stephen , Birhane Emiru 
Published: June 2023

AbstractLand rehabilitation through exclosures has been recognized as a promising practice in the restoration of degraded drylands. This study evaluated woody species density, diversity, cover, and biomass production from three regeneration approaches (EMTs): (1) Naturally Regenerated (ENR) exclosures, (2) exclosures with Soil and Water Conservation Structures (ECNR), and (3) those enriched with exotic and local plant species in addition to SWC structures (ECP) among which empirical evidence of comparisons are lacking for appropriate decision making of which restoration strategy to promote. Primary data were collected from a total of 72 study plots located across transects laid within exclosures and in adjacent grazing areas with similar ecological condition. Interview with 331 households and focus groups was used to further understand the effects. Regression analysis revealed that the mean difference in herbaceous and canopy cover, herbaceous and woody biomass, and woody species density and diversity of the three EMTs was statistically significantly higher than in the adjacent open grazing areas. Moreover, herbaceous biomass in ECP differed significantly from that of ECNR. The results indicated that ENR and ECP equally enhanced the selected vegetation parameters. This is predominantly attributed to the comparable responsiveness of many degraded areas to natural regeneration as they do to active regeneration approaches. Implementation of natural regeneration is suitable for immediate recruitment of strategy in terms of resources, time, and meeting objectives before decisions are made for assisted models of exclosure that require massive campaigns to construct soil and water conservation structures and huge seedling production in the dryland areas.
Peptides as “better biomarkers”? Value, challenges, and potential solutions to facilitate implementation
Authors: Latosinska Agnieszka , Frantzi Maria , Siwy Justyna 
Published: June 2023

AbstractPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry‐based platforms, specifically those studies that focus on the application of peptides as biomarkers to improve clinical management. We emphasize on the critical evaluation of their clinical value, how close they are to implementation, and the associated challenges and potential solutions to facilitate clinical implementation. During the last 5 years, numerous studies have been published, demonstrating the increased interest in mass spectrometry for the assessment of endogenous peptides as potential biomarkers. Importantly, the presence of few successful examples of implementation in patients' management and/or in the context of clinical trials indicates that the peptide biomarker field is evolving. Nevertheless, most studies still report evidence based on small sample size, while validation phases are frequently missing. Therefore, a gap between discovery and implementation still exists.
An improved method for intracellular DNA (iDNA) recovery from terrestrial environments
Authors: Medina Caro Diego , Horstmann Lucas , Ganzert Lars , Oses Romulo , Friedl Thomas , Wagner Dirk 
Published: June 2023

AbstractThe simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can help to separate the living in situ community (represented by iDNA) from background DNA that originated both from past communities and from allochthonous sources. As iDNA and eDNA extraction protocols require separating cells from the sample matrix, their DNA yields are generally lower than direct methods that lyse the cells within the sample matrix. We, therefore, tested different buffers with and without adding a detergent mix (DM) in the extraction protocol to improve the recovery of iDNA from surface and subsurface samples that covered a variety of terrestrial environments. The combination of a highly concentrated sodium phosphate buffer plus DM significantly improved iDNA recovery for almost all tested samples. Additionally, the combination of sodium phosphate and EDTA improved iDNA recovery in most of the samples and even allowed the successful extraction of iDNA from extremely low‐biomass iron‐bearing rock samples taken from the deep biosphere. Based on our results, we recommend using a protocol with sodium phosphate in combination with either a DM (NaP 300 mM + DM) or EDTA (NaP + EDTA 300 mM). Furthermore, for studies that rely on the eDNA pool, we recommend using buffers solely based on sodium phosphate because the addition of EDTA or a DM resulted in a decrease in eDNA for most of the tested samples. These improvements can help reduce community bias in environmental studies and contribute to better characterizations of both modern and past ecosystems.