bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-specific modulation of mitochondrial respiration and metabolism by the pro-apoptotic Bcl-2 family members Bax and Bak
Authors: Sovilj Dana , Kelemen Cristina Daniela , Dvorakova Sarka , Zobalova Renata , Raabova Helena , Kriska Jan , Hermanova Zuzana , Knotek Tomas , Anderova Miroslava , Klener Pavel , Filimonenko Vlada , Neuzil Jiri , Andera Ladislav 
Published: July 2023

Abstract
Proteins from the Bcl-2 family play an essential role in regulation of apoptosis. However, they also possess cell death-unrelated activities that are less well understood. This prompted us to study apoptosis-unrelated activities of the Bax and Bak, pro-apoptotic members of the Bcl-2 family. We prepared Bax/Bak-deficient human cancer cells of different origin and found that while respiration in the glioblastoma U87 Bax/Bak-deficient cells was greatly enhanced, respiration of Bax/Bak-deficient B lymphoma HBL-2 cells was slightly suppressed. Bax/Bak-deficient U87 cells also proliferated faster in culture, formed tumours more rapidly in mice, and showed modulation of metabolism with considerably increased NAD+/NADH ratio. Follow-up analyses documented increased/decreased expression of mitochondria-encoded subunits of respiratory complexes and stabilization/destabilization of the mitochondrial transcription elongation factor TEFM in Bax/Bak-deficient U87 and HBL-2 cells, respectively. We propose that (post)translational regulation of TEFM levels in Bax/Bak-deficient cells modulates levels of subunits of mitochondrial respiratory complexes that, in turn, contribute to respiration and the accompanying changes in metabolism and proliferation in these cells.
Upcycling of Polylactic acid to 2-Bromopropionates and Lactates
Authors: Liang Jianhao , Tang Peifeng , Fu Jinxia , Lan Xinmeng , Wu Yu , Zhang Qin , Kang Shimin 
Published: July 2023

Abstract
The valorization and recycling of bioderived polylactic acid (PLA) have garnered significant attentions. In this study, a pioneering approach using hydrogen bromide (HBr) catalysis for the degradation of PLA to 2-bromopropionates and lactates is presented. Both HBr solution and HBr-HAc (acetic acid) solution were found to be efficient for the process. Notably, the HBr solution predominantly yields lactic acid (LA) with an exceptional yield of ≥ 99 mol% at temperatures ranging from 80 to 110 oC. Conversely, the HBr-HAc solution yields both 2-bromopropionic acid (2BA) and LA, with 2BA yield achieving a remarkable yield of 55.2 mol% at 100 oC. Subsequently, esterification of the PLA derived 2BA and LA was conducted, and a serious of 2-bromopropionates and lactates were synthetized. Furthermore, the impact of reaction factors was investigated via multivariate analysis methods.
Secured Vanet Using Block Chain-sdn Controller and MF-WWO-MCC
Authors: S Thenappan , A Arun C. 
Published: July 2023

Abstract

The development of Vehicle Ad hoc NETwork (VANET) has resulted from the Intelligent Transportation System (ITS). This makes vehicles communicate with each other and provides solutions to the hectic traffic problem. This paper proposes a blockchain-based edge computing framework using Matching Factor-Water Wave Optimization-Montgomery Curve Cryptography (MF-WWO-MCC) to achieve secured communication. Initially, all the vehicles on the road are initialized; also, their details are registered in the blockchain using Inverse-3times Rotate Right Secure Hashing Algorithm (I-3RR-SHA-512). The trustworthiness of the user is evaluated when the user(vehicle) enters into a new Road Side Unit (RSU). Next, for data requests, the authentication of the normal user is verified. Then, by employing the Intuitionistic Fuzzy Set-K-Means Algorithm (IFS-KMA), which assigns RSU as cluster centroids, the vehicles are clustered. Next, through the Software Defined Network (SDN) controller, the requests are transferred to the RSU. In the end, by employing MF-WWO-MCC, the encrypted data is forwarded to the requested user vehicle. The proposed techniques’ performance is validated by analogizing against the prevailing methodologies.
A retrospective cohort study on the impact of the COVID-19 global pandemic on assessments and hospital admissions within a specialist adolescent eating disorder service
Authors: Wadsworth Robynne , Hochard Kevin , Doyle Lesley , Watkin Anna , Jaydeokar Sujeet 
Published: July 2023

Abstract
Eating disorder services worldwide were impacted by the COVID-19 pandemic experience, including its associated counter-measures. We examined the effect of the pandemic on sociodemographic and clinical characteristics of young people assessed and admitted by a specialist adolescent eating disorder service in the Northwest of England (UK). Routinely collected electronic data in the two years following the World Health Organisation declaration of a global pandemic in March 2020, was compared with data collected in the preceding two years.
This service experienced a 21% increase in assessments from the two years pre-COVID (n=78) to the two years following the pandemic onset (n=120), Inpatient admissions also increased by 10%. The pandemic had no effect on admission setting, with approximately 20% admitted to specialist eating disorder units, 40% to paediatric wards and 40% to adolescent mental health units, consistently across the four years. No significant difference in sociodemographic characteristics of young people within the service before or during the pandemic was found. However, self-rated eating disorder pathology at assessment was significantly more severe following the pandemic. Fewer admission had a recorded comorbid diagnosis of an Autistic Spectrum Condition following the pandemic compared to those admitted pre-pandemic.
These findings quantify the increase in demand for highly specialist adolescent eating disorder services since the start of the COVID-19 pandemic, with a higher severity of psychopathology managed outside of hospital. This has implications for service design, allocation of resources and future delivery of appropriate models of care to young people.
Multiple Giant Cystic-solid Hemangioblastomas in the Cerebellopontine Angle and Bilateral Cerebellar Hemispheres: Case Report and Literature Review
Authors: Song Lvmeng , Wu Xia , Xia Shixue , Cheng Yinchuan , Xia Xiaochao , Wang Zihao , Li Shun 
Published: July 2023

Abstract

Multiple giant cystic-solid hemangioblastomas (HMGs) located in the cerebellopontine angle (CPA) and bilateral cerebellar hemispheres are extremely rare. This article describes a 54-year-old female patient who was admitted to the hospital with "headache with hearing loss in both ears for 2 months". Physical examination showed that the patient was conscious and Romberg’s sign was positive. Magnetic resonance imaging (MRI) suggested multiple irregular cystic-solid abnormal signals in the right CPA and bilateral cerebellar hemispheres. A complete resection of the tumor was performed via midline suboccipital approach, and the patient's headache symptoms were relieved significantly after the operation, and the cranial nerve function was well protected. The diagnosis of hemangioblastoma was supported by the results of postoperative immunohistochemical staining.
Mesenteric and omental lymphatic malformations in children: seven-year surgical experience from two centers in China
Authors: Yan Jiayu , Fu Yao , Liu Shuting , Bai Yuzuo , Chen Yajun 
Published: July 2023

Abstract
Purpose To compare the clinical characteristics, surgical management and prognosis of mesenteric lymphatic malformations (ML) and omental lymphatic malformations (OL) in children.
Methods This retrospective study included 148 ML patients and 53 OL patients who underwent surgical treatment at two centers between January 2016 and December 2022. Details of the patients’ clinical characteristics, cyst characteristics, preoperative complications, surgical methods, and prognosis were retrieved and compared.
Results No significant differences between ML and OL patients were noted in sex ratio, prenatal diagnosis, and age of diagnosis. The presence of vomiting was more common in ML patients than in OL patients (46.6% vs. 22.6%, P = 0.002), but OL patients were more likely to be misdiagnosed (35.8% vs. 18.8%, P = 0.012). The size of cysts in OL patients was significantly larger than that in ML patients (14.0 [8.0, 17.3] vs. 10.0 [6.0, 13.0] cm, P＜0.001), and cysts with turbid fluid were more common in OL patients (38.0% vs. 20.6%, P＜0.001). Cyst excision was performed in 137 (92.6%) ML patients and 51 (96.2%) OL patients, and the incidence of postoperative complications was lower (12.6% vs. 4.2%, P = 0.165) among the latter. The main postoperative complications included adhesive ileus and recurrence of cysts. Additionally, laparoscopic surgery was more commonly undergone among OL patients (69.8% vs. 39.2%, P＜0.001).
Conclusions Compared with ML patients, OL patients are more prone to misdiagnosis and infection, but the prognosis of surgical treatment is better. Laparoscopic cyst excision may be the preferred treatment for OL patients.
A membrane filtration method for the enumeration of Escherichia coli in bathing water and other waters with high levels of background bacteria
Authors: Kemper Merel A. , Veenman Christiaan , Blaak Hetty , Schets Franciska M. 
Published: July 2023

Abstract

The presence and level of faecal indicator bacteria are important factors in estimating the microbiological quality of surface water and the risk of human infection upon exposure to this water. Until 2014, ISO 9308-1:2000 was available and used to enumerate faecal indicator Escherichia coli in bathing water. In 2014, this ISO was technically revised and replaced by ISO 9308-1:2014. This ISO introduced a less selective method for enumeration of E. coli that allows non-specific growth from waters containing high levels of bacteria, such as surface waters. This implies that currently there is no suitable reference membrane filtration method for the compliance monitoring of official bathing sites for E. coli according to the European Bathing Water Directive. Here, the performance characteristics of three chromogenic culture media, namely Tryptone Bile X-glucuronide (TBX) agar, Chromogenic Coliform Agar (CCA), and CHROMagar E. coli/Coliform (ECC) were investigated at 44 °C for water with varying levels of bacteria according to ISO 13843:2017. Based on performance characteristics, colony counts, and practical usage, TBX appeared the most suitable culture medium for the enumeration of E. coli in bathing water and other waters with high levels of background bacteria, such as surface water in agricultural areas and wastewater discharge points.
The Efficacy of Dietary Approaches to Stop Hypertension (DASH) Diet on Lipid Profile: A Systematic Review and Meta-analysis of Clinical Controlled Trials
Authors: Sahebkar Amirhossein , Heidari Zahra , Kiani Zahra , Atefi Masoumeh , Zareie Azadeh , Shojaei Mehrnaz , Askari Gholamreza , Kesharwani Prashant , Bagherniya Mohammad 
Published: July 2023

Background:
Dyslipidemia is considered a causal risk factor for coronary heart disease
and stroke. Plant-based diets such as dietary approaches to stop hypertension (DASH) have beneficial
effects on cardiovascular health. This meta-analysis was conducted to assess the effects of the
DASH diet on lipid profiles based on clinical controlled trials.

Methods:
An inclusive online search was performed in medical databases including Web of Science,
PubMed, Scopus, and Google Scholar up to October 2021 to identify trials assessing the effect
of the DASH diet on lipid profiles.

Results:
Seventeen studies comprising 2218 individuals were included in this meta-analysis. In
comparison to the control group, following the DASH diet resulted in a significant reduction in
serum triglycerides (WMD: -5.539 mg/dl; 95% CI: -8.806, -2.272) and low-density lipoprotein
cholesterol (WMD: -6.387 mg/dl; 95% CI: -12.272, -0.501). However, DASH diet could not reduce
serum total cholesterol (WMD: -5.793 mg/dl; 95% CI: -12.84, 1.254), high-density lipoprotein
cholesterol (WMD: 0.631 mg/dl; 95% CI: -0.749, 2.011), and total cholesterol/high-density
lipoprotein cholesterol ratio (WMD: -0.11 mg/dl; 95% CI: -0.27, 0.05).

Conclusion:
Findings of this meta-analysis showed that following the DASH diet had beneficial effects
on serum triglycerides and low-density lipoprotein cholesterol, however, it had no effect on
serum total cholesterol and high-density lipoprotein cholesterol levels. Based on these results, the
DASH diet can be considered a strategy for the prevention and complementary management of dyslipidemia.
Fluvastatin: A Choice for COVID-19-associated Mucormycosis Management
Authors: Tavakkoli Alireza , Johnston Thomas P. , Sahebkar Amirhossein 
Published: July 2023

:
SARS-CoV-2 invades the respiratory tract epithelium and can result in systemic
inflammation prior to an infection caused by either bacteria or fungus. COVID-19-
associated mucormycosis (CAM) is a serious condition that can occur during the time of
the disease due to increased administration of corticosteroids. Various studies have
suggested that statins may improve clinical outcomes in COVID-19 patients. According
to several preclinical reports, fluvastatin was shown to exert direct and indirect synergistic
antifungal activity. Thus, fluvastatin could be considered a potential antifungal agent
when no other option is available. Furthermore, in comparison with other statins, fluvastatin
exhibits the fewest drug/drug interactions with anti-Mucorales azoles (e.g., isavuconazole
and posaconazole), as well as with medicines that are used in solid organ transplant
recipients (e.g., cyclosporine) and HIV-positive individuals (e.g., ritonavir); two groups
of patients that have a higher risk of infection with Mucorales fungi following a SARSCoV-
2 infection.
Linoleic and Arachidonic Fatty Acids and their Potential Relationship
with Inflammation, Pregnancy, and Fetal Development
Authors: Ortiz Macarena , Álvarez Daniela , Muñoz Yasna , Crisosto Nicolás , Valenzuela Rodrigo , Maliqueo Manuel 
Published: July 2023

Abstract:
A healthy maternal diet must consider an appropriate supply of long-chain polyunsaturated
fatty acids (LCPUFAs) precursors to ensure adequate growth and development
of the fetus. In this regard, n-6 PUFAs, predominantly linoleic (C18:2 n-6, LA) and
arachidonic acid (C20:4 n-6), have a central role in the development of the central nervous
system because they are part of the membrane structure and participate in the
metabolism and signal transduction of cells. Nevertheless, they can also be transformed
into inflammatory metabolites promoting the pathogenesis of cardiovascular diseases,
cancer, and autoimmune or inflammatory conditions. In modern westernized societies,
there is a high dietary consumption of foods rich in n-6 PUFAs which could have detrimental
consequences for the fetus and neonate due to excessive exposure to these fatty
acids (FAs).

Objective:
To summarize the evidence of maternal, placental, and fetal alterations that an
excessive intake of n-6 polyunsaturated FAs (PUFAs), LA, and AA, could produce during
pregnancy.

Methods:
A thorough review of the literature regarding the effects of n-6 PUFAs during
pregnancy and lactation including in vivo and in vitro models, was carried out using the
PubMed database from the National Library of Medicine-National Institutes of Health.

Results:
An elevated intake of n-6 PUFA, specifically LA, during pregnancy influences
children's motor, cognitive, and verbal development during infancy and early childhood.
Similarly, they could harm the placenta and the development of other fetal organs such
as the fat tissue, liver, and cardiovascular system.

Conclusion:
Maternal diet, specifically LA intake, could have significant repercussions
on fetal development and long-term consequences in the offspring, including the possibility
of future metabolic and mental diseases. It would be necessary to focus on the prevention
of these alterations through timely dietary interventions in the target population.
HILIC UPLC/ QTof MS Method Development for the Quantification of
AGEs Inhibitors - Trouble Shooting Protocol
Authors: Velichkova Stefaniya , Foubert Kenn , Theunis Mart , Pieters Luc 
Published: July 2023

Objective:
The paper reports an attempt to develop and validate a HILIC UPLC/ QTof
MS method for quantifying N-ε-carboxymethyl-L-lysine (CML) in vitro, testing N-ε-
carboxy[D2]methyl-L-lysine (d2-CML), and N-ε-carboxy[4,4,5,5-D4]methyl-L-lysine (d4-CML) as
internal standards.

Method:
During the method development, several challenging questions occurred that hindered
the successful completion of the method. The study emphasizes the impact of issues, generally
overlooked in the development of similar analytical protocols. For instance, the use of glassware
and plasticware was critical for the accurate quantification of CML. Moreover, the origin of atypical
variation in the response of the deuterated internal standards, though widely used in other experimental
procedures, was investigated.

Result:
A narrative description of the systematic approach used to address the various drawbacks
during the analytical method development and validation is presented.

Conclusion:
Reporting those findings can be considered beneficial while bringing an insightful notion
about critical factors and potential interferences. Therefore, some conclusion and ideas can be
drawn from these trouble-shooting questions, which might help other researchers to develop more
reliable bioanalytical methods, or to raise their awareness of stumbling blocks along the way.
Is there a Relation between Depression and Anxiety to Clinical Characteristics
among Patients with T2D in Greece?
Authors: Theofilou Paraskevi 
Published: July 2023

Background:
Diabetes is a chronic disease that may be related to various periods of an
individual’s life.

Aim:
The aim of the present study is the investigation of the existence of depression and anxiety
among patients suffering from type 2 diabetes as well as the factors that influence these variables
(depression and anxiety).

Method and Material:
The Hospital Anxiety and Depression Scale (HADS) for mental health assessment
was used in the context of the collection of the research data. The study involved 100 patients
(42 men and 58 women) with an average age of 63.72 ± 9.84 years of life.

Result:
The results showed that there was a positive correlation between HbA1c value and anxiety
as well as HADS questionnaire total score, and a positive correlation between blood glucose value
and anxiety as well as HADS questionnaire total score.

Conclusion:
Both depression and anxiety of these patients are influenced by different clinical factors.
Assessment of Knowledge and Perception of Sodium-Glucose Co-transporter 2 (SGLT-2) Inhibitors Prescription among Physicians in Saudi Arabia
Authors: Somaili Mohammed , Oraibia Omar , Darraj Majed , Hassan Amal , Moafa Esaam , Kulaybi Abdulrahman , Shubayli Sahar , Moafa Razan , Mghfori Ghadah , Jaafari Afaf , Somily Majed 
Published: July 2023

Background:
Sodium-glucose cotransporter 2 inhibitors are a new class of medications
that have been proven to improve both glycemic control and cardio-renal outcomes. The
knowledge, attitude, and perception toward their prescriptions in Jazan, Saudi Arabia, are still unknown.

Objective:
The study aimed to measure the level of knowledge and attitude toward sodium-glucose
cotransporter 2 inhibitors prescription among physicians in the Jazan region, Saudi Arabia.

Methods:
Data analysis was performed using Statistical Package for the Social Sciences, SPSS 23rd
version. Frequency and percentages were used to display categorical variables. Minimum, maximum,
mean, and standard deviation were used to test numerical variables. Independent t-test and
ANOVA test were both utilized to test the factors associated with knowledge and attitude toward
the use of SGLT-2 inhibitors.

Results:
A total of 65 participants were included in the study. 26.2% had a low knowledge level,
30.8% had a moderate knowledge level, and 43.1% had a high knowledge level of sodium-glucose
cotransporter 2 inhibitors. 9.2% had a low attitude level, 43.1% had a moderate attitude level, and
47.7% had a high attitude level toward sodium-glucose cotransporter 2 inhibitors. Age, professional
status, years of experience, and specialty were significantly associated with attitude but not with
the knowledge of sodium-glucose cotransporter 2 inhibitors prescription.

Conclusion:
While the study cohort scored high in the knowledge and attitude domains of the survey,
a large proportion failed to answer very essential questions in type 2 diabetes management.
An educational awareness program needs to be carried out to strengthen the physicians’ knowledge
of SGLT2 inhibitors prescription.
Clinical features of ischemic heart disease in patients after COVID-19 infection and coronary artery bypass graft surgery
Authors: Loginova A. O. , Tarlovskaya E. I. , Ancigina L. N. , Pimenova P. V. 
Published: July 2023

Aim. To evaluate whether the history of coronavirus disease 2019 (COVID-19) in patients undergone cardiac surgery (coronary artery bypass grafting - CABG) causes alterations in their clinical and laboratory status.Materials and methods. Clinical data of 42 patients undergone CABG in 2019 were analyzed. Informed written consent was obtained from all participants in accordance with the Declaration of Helsinki. Determination of COVID-19 positive or negative status was performed due to results of nasal and throat swabs using reverse transcriptase-polymerase chain reaction (RT-PCR) or positive serum COVID-19 antibodies. Statistical analyses were performed using Jamovi software.Results. In COVID-19 positive patients compared to COVID-19 negative patients: the level of serum cholesterol was significantly higher: 5,22 [4,03; 6,22] vs. 4.06 [3,56; 4,88] mmol/L, р = 0,005; OR 0,492 [0,282; 0,858] 95% CI, p ratio 0,012; the level of low-density lipoproteins was significantly higher: 3,27 [2,16; 3,96] vs. 2,28 [1,60; 3,08] mmol/L, p 0,014; OR 0,518 [0,294; 0,914] 95% CI, p ratio 0,023; the incidence of chronic kidney disease stage C3a (CKD) was higher: 7 (16,7%) vs. 1 (2,4%), p 0,008; OR 0,0779 [0,00855; 0,710] 95% CI, p ratio 0,024; tendency to a higher serum creatinine: 89,7 [83,0; 105,0] vs. 81,0 [75,0; 90,5] μmol/L, p 0,060; OR 0,976 [0,945; 1,01] 95% CI, p ratio 0,153; tendency to a lower glomerular filtration rate using CKD-EPI: 66,1 ± 17,3 vs. 75,7 ± 16,1 ml/min/1,73m2, p 0,034; OR 1,0368 [0,9962; 1,08] 95% CI, p ratio 0,076; tendency to a higher serum D-dimer level: 154 [104; 364] vs. 137 [97; 173] ng/ml, p 0,07; OR 0,997 [0,994; 1,0] 95% CI, p ratio 0,141; tendency to a higher mean heart rate 75 [71; 79,5] vs. 72 [63; 74,5] bpm, p 0,026; PR 0,951 [0,886; 1,02] 95% CI, p ratio 0,169. Heart rate variability parameters haven’t shown statistical significance between groups.Conclusion. Patients with a history of COVID-19 who underwent CABG had alterations in their clinical and laboratory status. These alterations should be thoroughly investigated to make a forehanded change in their therapy.