bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Himalayan marmot distribution and plague risk in Qinghai province of China using the “3S” technology
Authors: Wu Hailian , Wu Haisheng , Wang Yongshun , Li Hongying , Tian Fuzhang , Zhou Kuizhang , Qi Zhizhen , Zhang Yiquan , Zhang Qingwen , Zhang Xuefei 
Published: February 2023

AbstractTo provide guidance for plague surveillance and a reliable basis for plague prevention and control, we analyzed the habitat characteristics of Himalayan marmots, developed Himalayan marmot information collection system V3.0 based on global navigation satellite system (GNSS), remote sensing, and geographic information system (“3S”) technology, and drew a predictive spatial distribution map of Himalayan marmots in Qinghai Province. Field survey data of 352 marmot plague sites in Qinghai Province were collected in 2014, and the data from 80 sample sites were included. The Himalayan marmot habitat characteristics were analyzed based on “3S” technology using five environment variables (elevation, slope, aspect, vegetation cover, and grass type) and the geographical coordinates. Himalayan marmot information collection system V3.0, which has been approved by the National Copyright Administration of the People’s Republic of China (No.00764743), was used to draw a predictive spatial distribution map of Himalayan marmots in Qinghai province. Moreover, from 2015 to 2017, positioning data of the plague-foci and plague-free areas in Qinghai Province were collected using GNSS receptor for field validations to verify the accuracy of the marmot predictive spatial distribution map. Elevation, slope, vegetation cover, and grassland type were identified as important environmental factors that determine the spatial distribution of Himalayan marmots. The suitable range of environmental features was 3400–4600 m elevation, 5°–20° slope, 0.60–1.00 vegetation cover, and alpine meadows. The Himalayan marmot predictive spatial distribution map in Qinghai Province based on “3S” technology and marmot information collection system V3.0 had a spatial resolution of 30 m. Field validation in areas of Qinghai Province revealed a prediction accuracy and mean absolute error of 0.8669 and 0.1331, respectively, which indicated excellent prediction accuracy. This study greatly improved the work efficiency of plague surveillance and effectively reduced the work intensity of researchers. Application of “3S” technology and marmot information collection system V3.0 has improved the data collection efficiency, provided new technical means for plague investigation and research, and provided a reference for development of plague surveillance programs. The research results will play a positive role in promoting the improvement and perfection of plague prevention and control strategies in Qinghai province and even in China.
Combined space stressors induce independent behavioral deficits predicted by early peripheral blood monocytes
Authors: Rienecker Kira D. A. , Grue Katherine , Paladini Maria Serena , Frias Elma S. , Frattini Valentina , Borlongan Mia C. , Chou Austin , Torres-Espin Abel , Krukowski Karen , Ferguson Adam R. , Rosi Susanna 
Published: January 2023

AbstractInterplanetary space travel poses many hazards to the human body. To protect astronaut health and performance on critical missions, there is first a need to understand the effects of deep space hazards, including ionizing radiation, confinement, and altered gravity. Previous studies of rodents exposed to a single such stressor document significant deficits, but our study is the first to investigate possible cumulative and synergistic impacts of simultaneous ionizing radiation, confinement, and altered gravity on behavior and cognition. Our cohort was divided between 6-month-old female and male mice in group, social isolation, or hindlimb unloading housing, exposed to 0 or 50 cGy of 5 ion simplified simulated galactic cosmic radiation (GCRsim). We report interactions and independent effects of GCRsim exposure and housing conditions on behavioral and cognitive performance. Exposure to GCRsim drove changes in immune cell populations in peripheral blood collected early after irradiation, while housing conditions drove changes in blood collected at a later point. Female mice were largely resilient to deficits observed in male mice. Finally, we used principal component analysis to represent total deficits as principal component scores, which were predicted by general linear models using GCR exposure, housing condition, and early blood biomarkers.
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
Authors: Singh Sameer , Banerjee Anoosha , Vanden Broeck Arnaud , Klinge Sebastian 
Published: January 2023

AbstractOne of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.
Changes in pupil dilation and P300 amplitude indicate the possible involvement of the locus coeruleus-norepinephrine (LC-NE) system in psychological flow
Authors: Lu Hairong , van der Linden Dimitri , Bakker Arnold B. 
Published: February 2023

AbstractPsychological flow is a state of full task immersion. The present study was conducted to test the hypothesis that psychological flow is positively related to activity of the phasic locus coeruleus-norepinephrine (LC-NE) system, which supports decisions on whether to engage in or disengage from the current activity. Subjective flow was assessed among 36 participants who engaged in a gamified version of the n-back task with various difficulty levels (0, 1, 2, and 3 back). During the tasks, continuous pupil diameter and EEG were recorded. We found that psychological flow and two presumed indicators of the phasic LC-NE activity (pupil dilation and EEG P300 amplitude) fit inverted U-shapes with increasing subjective task difficulty. Moreover, a positive linear relationship between psychological flow and pupil dilation (not with P300) was found. In conclusion, this study indicates the involvement of the LC-NE system in the peak experience of flow.
Adaptation of Glycyrrhiza glabra L. to water deficiency based on carbohydrate and fatty acid quantity and quality
Authors: Haghighi Tahereh Movahhed , Saharkhiz Mohammad Jamal , Kavoosi Gholamreza , Zarei Mehdi 
Published: January 2023

AbstractWater deficit affects agricultural systems negatively globally. This research objective was to mitigate drought’s detrimental effects on plants metabolite profiling by utilizing biofertilizers and mineral nutrition. The carbohydrate content and fatty acid profile of Licorice (Glycyrrhiza glabra) were assessed under Silicon (Si) nutrition, Claroiedoglomus etunicatum inoculation (F), and drought stress (100, 80, 60, 40, and 20% of field capacity (FC)). Results showed that Si application increased total sugar content under severe drought levels (20 and 40% FC) and made it reach 12.41 and 12.63 g/100 g DW, respectively. Sucrose, as the predominant sugar of licorice, was at its highest level (13.1 g/100 g DW) in response to integrated values of F and Si (60% FC). Gas chromatography–mass spectrometry showed that the majority of fatty acid components in plants were 9-Octadecenoic acid (8.72–71.27%), 9,12-Octadecadienoic acid (0.1–56.43%), Hexadecanoic acid (12.84–30.59%), Octadecanoic acid (6.9–15.3%), Docosanoic acid (0.57–2.77%), Eicosanoic acid (1.07–2.64%), and 7-Hexadecenoic acid (0.26–2.62%). Since a lower omega6/omega3 ratio represents a healthier product, the lowest ratio (0.25%) was observed in well-watered inoculated plants. Also, severe drought-treated plants under integrated Si and F applications showed a low omega6/omega3 ratio (1.88%). In conclusion, Si and F improved synergistically the carbohydrate content and fatty acid profile in plants, despite the drought stress.
Ultrastructural changes in esophageal tissue undergoing stretch tests with possible impact on tissue engineering and long gap esophageal repairs performed under tension
Authors: Biro Ede , Sommer Gerhard , Leitinger Gerd , Abraham Hajnalka , Kardos Daniel J. , Oberritter Zsolt , Saxena Amulya K. 
Published: January 2023

AbstractEsophageal biomechanical studies are being performed to understand structural changes resulting from stretches during repair of esophageal atresias as well as to obtain biomechanical values for tissue-engineered esophagus. The present study offers insights into ultrastructural changes after stretching of the ovine esophagus using uniaxial stretch tests. In vitro uniaxial stretching was performed on esophagi (n = 16) obtained from the abattoir within 4–6 h of 1-month-old lambs. Esophagi were divided into 4 groups (4 esophagi/group): control, Group1 (G1), Group2 (G2), Group3 (G3) stretched to 20%, 30% and 40% of their original length respectively. Force and lengthening were measured with 5 cycles performed on every specimen. Transmission electron microscopic (TEM) studies were performed on the 4 groups. During observational TEM study of the control group there were no significant differences in muscle cell structure or extracellular matrix. In all stretched groups varying degrees of alterations were identified. The degree of damage correlated linearly with the increasing level of stretch. Distance between the cells showed significant difference between the groups (control (μ = 0.41 μm, SD = 0.26), G1 (μ = 1.36 μm, SD = 1.21), G2 (μ = 2.8 μm, SD = 1.83), and G3 (μ = 3.01 μm, SD = 2.06). The diameter of the cells (control μ = 19.87 μm, SD = 3.81; G1 μ = 20.38 μm, SD = 4.45; G2 μ = 21.7 μm, SD = 6.58; G3 μ = 24.48 μm, SD = 6.69) and the distance between myofibrils (control μ = 0.23 μm, SD = 0.08; G1 μ = 0.27 μm, SD = 0.08; G2 μ = 0.4 μm, SD = 0.15; G3 μ = 0.61 μm, SD = 0.2) were significantly different as well (p < 0.05 was considered to be significant). Esophageal stretching > 30% alters the regular intracellular and extracellular structure of the esophageal muscle and leads to disruption of intra- and extracellular bonds. These findings could provide valuable insights into alterations in the microscopic structure of the esophagus in esophageal atresias repaired under tension as well as the basis for mechanical characterization for tissue engineering of the esophagus.
In vivo and in vitro toxicity profile of tetrabutylammonium bromide and alcohol-based deep eutectic solvents
Authors: Inayat Shamaila , Ahmad Sajid Rashid , Awan Sana Javaid , Nawshad Muhammad , Ali Qurban 
Published: January 2023

AbstractDeep eutectic solvents (DESs) have emerged as new promising solvents in the field of “green chemistry,” which possess a broad range of potential applications. However, the ecotoxicological profile of these solvents is still poorly known. In this study, ammonium-based deep eutectic solutions with glycerol (2:2), ethylene glycol (1:2), and diethylene glycol (1:2) as hydrogen bond donors in 1:2 proportion were evaluated for their interaction with various biological systems, including gram-positive and negative bacteria, fungi, fish, and human fibroblast cell lines. The DES synthesis was confirmed by Fourier transform infrared spectroscopy analysis, which analyses the interactions between DES precursors for their synthesis. The antimicrobial activity of tetrabutylammonium bromide: ethylene glycol was the most potent, while tetrabutylammonium bromide: diethylene glycol had a higher LC50 against C. carpio fish. Tetrabutylammonium bromide: glycerol was supposed to be the most suitable DES in terms of cell viability percentage (118%) and 2,2-diphenyl-1-picrylhydrazyl scavenging activity (93%). Finally, tetrabutylammonium bromide in glycerol can be considered an eco-friendly solvent due to its lower toxicity in both in vivo and in vitro environments.
Fully automated immunoassay for cholesterol uptake capacity to assess high-density lipoprotein function and cardiovascular disease risk
Authors: Murakami Katsuhiro , Harada Amane , Toh Ryuji , Kubo Takuya , Miwa Keiko , Kim Jeeeun , Kiriyama Maria , Iino Takuya , Nishikawa Youichi , Uno Shin-Nosuke , Akatsuchi Kohei , Nagao Manabu , Ishida Tatsuro , Hirata Ken-ichi 
Published: February 2023

AbstractHigh-density lipoprotein (HDL) cholesterol efflux capacity (CEC), which is a conventional metric of HDL function, has been associated with coronary heart disease risk. However, the CEC assay requires cultured cells and takes several days to perform. We previously established a cell-free assay to evaluate cholesterol uptake capacity (CUC) as a novel measure of HDL functionality and demonstrated its utility in coronary risk stratification. To apply this concept clinically, we developed a rapid and sensitive assay system based on a chemiluminescent magnetic particle immunoassay. The system is fully automated, providing high reproducibility. Measurement of CUC in serum is completed within 20 min per sample without HDL isolation, a notably higher throughput than that of the conventional CEC assay. CUC decreased with myeloperoxidase-mediated oxidation of HDL or in the presence of N-ethylmaleimide, an inhibitor of lecithin: cholesterol acyltransferase (LCAT), whereas CUC was enhanced by the addition of recombinant LCAT. Furthermore, CUC correlated with CEC even after being normalized by ApoA1 concentration and was significantly associated with the requirement for revascularization due to the recurrence of coronary lesions. Therefore, our new assay system shows potential for the accurate measurement of CUC in serum and permits assessing cardiovascular health.
A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection
Authors: Arakawa Masashi , Yoshida Akiho , Okamura Shinya , Ebina Hirotaka , Morita Eiji 
Published: January 2023

AbstractProteases play critical roles in various biological processes, including apoptosis and viral infection. Several protease biosensors have been developed; however, obtaining a reliable signal from a very low level of endogenous protease activity remains a challenge. In this study, we developed a highly sensitive protease biosensor, named FlipNanoLuc, based on the Oplophorus gracilirostris NanoLuc luciferase. The flipped β-strand was restored by protease activation and cleavage, resulting in the reconstitution of luciferase and enzymatic activity. By making several modifications, such as introducing NanoBiT technology and CL1-PEST1 degradation tag, the FlipNanoLuc-based protease biosensor system achieved more than 500-fold luminescence increase in the corresponding protease-overexpressing cells. We demonstrated that the FlipNanoLuc-based caspase sensor can be utilized for the detection of staurosporine-induced apoptosis with sixfold increase in luminescence. Furthermore, we also demonstrated that the FlipNanoLuc-based coronavirus 3CL-protease sensor can be used to detect human coronavirus OC43 with tenfold increase in luminescence and severe acute respiratory syndrome-coronavirus-2 infections with 20-fold increase in luminescence by introducing the stem-loop 1 sequence to prevent the virus inducing global translational shutdown.
An open source Python library for environmental isotopic modelling
Authors: Hassanzadeh Ashkan , Valdivielso Sonia , Vázquez-Suñé Enric , Criollo Rotman , Corbella Mercè 
Published: February 2023

AbstractIsotopic composition modelling is a key aspect in many environmental studies. This work presents Isocompy, an open source Python library that estimates isotopic compositions through machine learning algorithms with user-defined variables. Isocompy includes dataset preprocessing, outlier detection, statistical analysis, feature selection, model validation and calibration and postprocessing. This tool has the flexibility to operate with discontinuous inputs in time and space. The automatic decision-making procedures are knitted in different stages of the algorithm, although it is possible to manually complete each step. The extensive output reports, figures and maps generated by Isocompy facilitate the comprehension of stable water isotope studies. The functionality of Isocompy is demonstrated with an application example involving the meteorological features and isotopic composition of precipitation in N Chile, which are compared with the results produced in previous studies. In essence, Isocompy offers an open source foundation for isotopic studies that ensures reproducible research in environmental fields.
Transforming Food Waste into a Resource
Authors: Segre Andrea , Gaiani Silvia 
Published: January 2023

An estimated 50% of the food produced worldwide is wasted. If the entire world had the same voracious appetite as Europeans, it would take three planets to produce the amount demanded.
In Britain, 18 million tonnes (14 billion pounds worth) of perfectly edible household food is thrown away every year. Apart from the waste, overeating is becoming a serious public health issue in many countries. According to a U.S. study, Western countries are consuming an average daily surplus of 1400 calories per person.
This easy to read manual on overproduction and overconsumption in contemporary society highlights some alarming global data. It discusses the origins and causes of food waste and its correlation with climate change, agriculture, population growth, and the need for a more ethical, ecological society. The complexity of the waste dynamic processes imposes a dire need to reconsider economic relationships and social values in the light of sustainability and eco-efficiency.
After a foreword by Vandana Shiva , the first two chapters describe the current imbalanced global food market. The book then goes on to present projects from around the world that are aimed at finding practical and efficient solutions to food waste. Particular emphasis is placed on Last Minute Market. This initiative links shops and producers with unsold food to people, and charities, in need. Originated at the University of Bologna Faculty of Agriculture, the project offers economic, environmental and social benefits. Alongside other European initiatives, it has contributed to a greener economy and a more sustainable world. Based on the data, and on the initiatives presented, the conclusion is devoted to the idea of a "new intelligent ecology".
Iron and mitochondria in the susceptibility, pathogenesis and progression of COPD
Authors: Faherty Lynne , Kenny Sarah , Cloonan Suzanne M. 
Published: February 2023

Abstract
Chronic obstructive pulmonary disease (COPD) is a debilitating lung disease characterised by airflow limitation, chronic bronchitis, emphysema and airway remodelling. Cigarette smoke is considered the primary risk factor for the development of COPD; however, genetic factors, host responses and infection also play an important role. Accumulating evidence highlights a role for iron dyshomeostasis and cellular iron accumulation in the lung as a key contributing factor in the development and pathogenesis of COPD. Recent studies have also shown that mitochondria, the central players in cellular iron utilisation, are dysfunctional in respiratory cells in individuals with COPD, with alterations in mitochondrial bioenergetics and dynamics driving disease progression. Understanding the molecular mechanisms underlying the dysfunction of mitochondria and cellular iron metabolism in the lung may unveil potential novel investigational avenues and therapeutic targets to aid in the treatment of COPD.
Visual Scene Displays for Children and Adults: Using Case Studies to Bridge Research and Clinical Practice
Authors: Hajjar David J. , Mulkerin Kathleen 
Published: February 2023

Purpose:
The purpose of this clinical focus article is to share research and clinical perspectives related to visual scene display (VSD) technology for people who use augmentative and alternative communication (AAC). Furthermore, the authors will help bridge the gap between research and clinical practice as speech-language pathologists (SLPs) consider supplementing AAC systems with VSD technology. The authors will review research and clinical practice about the development and use of VSD technology for both children and adults as a tool to support language, literacy, and social interactions. SLPs will be informed about key principles from VSD research to assist them to use this technology to supplement multimodal communication methods. Case studies will provide examples for SLPs to consider how they can create and use VSD technology in their clinical practice.

Conclusions:
Previous AAC research supports the development and use of VSDs for emerging communicators and people with aphasia. Based on the authors' clinical observations and review of the relevant research, challenges remain with VSD implementation as SLPs determine how to develop and effectively integrate this newer technology with current AAC interface design. Recommendations and ideas will be provided to SLPs who are considering VSDs as a visual support to promote language development and increase social interactions.
A novel observation points‐based positive‐unlabeled learning algorithm
Authors: He Yulin , Li Xu , Zhang Manjing , Fournier‐Viger Philippe , Huang Joshua Zhexue , Salloum Salman 
Published: February 2023

AbstractIn this study, an observation points‐based positive‐unlabeled learning algorithm (hence called OP‐PUL) is proposed to deal with positive‐unlabeled learning (PUL) tasks by judiciously assigning highly credible labels to unlabeled samples. The proposed OP‐PUL algorithm has three components. First, an observation point classifier ensemble (OPCE) algorithm is constructed to divide unlabeled samples into two categories, which are temporary positive and permanent negative samples. Second, a temporary OPC (TOPC) is trained based on the combination of original positive samples and permanent negative samples and then the permanent positive samples that are correctly classified with TOPC are retained from the temporary positive samples. Third, a permanent OPC (POPC) is finally trained based on the combination of original positive samples, permanent positive samples and permanent negative samples. An exhaustive experimental evaluation is conducted to validate the feasibility, rationality and effectiveness of the OP‐PUL algorithm, using 30 benchmark PU data sets. Results show that (1) the OP‐PUL algorithm is stable and robust as unlabeled samples and positive samples are increased in unlabeled data sets and (2) the permanent positive samples have a consistent probability distribution with the original positive samples. Moreover, a statistical analysis reveals that POPC in the OP‐PUL algorithm can yield better PUL performances on the 30 data sets in comparison with four well‐known PUL algorithms. This demonstrates that OP‐PUL is a viable algorithm to deal with PUL tasks.