bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging flow cytometry-based multiplex FISH for three IGH translocations in multiple myeloma
Authors: Tsukamoto Taku , Kinoshita Masaki , Yamada Kazuhiro , Ito Hodaka , Yamaguchi Toshikazu , Chinen Yoshiaki , Mizutani Shinsuke , Fujino Takahiro , Kobayashi Tsutomu , Shimura Yuji , Inazawa Johji , Kuroda Junya 
Published: March 2023

AbstractThree types of chromosomal translocations, t(4;14)(p16;q32), t(14;16)(q32;q23), and t(11;14)(q13;q32), are associated with prognosis and the decision making of therapeutic strategy for multiple myeloma (MM). In this study, we developed a new diagnostic modality of the multiplex FISH in immunophenotyped cells in suspension (Immunophenotyped-Suspension-Multiplex (ISM)-FISH). For the ISM-FISH, we first subject cells in suspension to the immunostaining by anti-CD138 antibody and, then, to the hybridization with four different FISH probes for genes ofIGH,FGFR3,MAF, andCCND1tagged by different fluorescence in suspension. Then, cells are analyzed by the imaging flow cytometry MI-1000 combined with the FISH spot counting tool. By this system of the ISM-FISH, we can simultaneously examine the three chromosomal translocations, i.e, t(4;14), t(14;16), and t(11;14), in CD138-positive tumor cells in more than 2.5 × 104nucleated cells with the sensitivity at least up to 1%, possibly up to 0.1%. The experiments on bone marrow nucleated cells (BMNCs) from 70 patients with MM or monoclonal gammopathy of undetermined significance demonstrated the promising qualitative diagnostic ability in detecting t(11;14), t(4;14), and t(14;16) of our ISM-FISH, which was more sensitive compared with standard double-color (DC) FISH examining 200 interphase cells with its best sensitivity up to 1.0%. Moreover, the ISM-FISH showed a positive concordance of 96.6% and negative concordance of 98.8% with standard DC-FISH examining 1000 interphase cells. In conclusion, the ISM-FISH is a rapid and reliable diagnostic tool for the simultaneous examination of three critically importantIGHtranslocations, which may promote risk-adapted individualized therapy in MM.
Two-step structural changes in M3 muscarinic receptor activation rely on the coupled Gq protein cycle
Authors: Kim Yong-Seok , Yeon Jun-Hee , Ko Woori , Suh Byung-Chang 
Published: March 2023

AbstractG protein-coupled receptors (GPCRs) regulate diverse intracellular signaling pathways through the activation of heterotrimeric G proteins. However, the effects of the sequential activation–deactivation cycle of G protein on the conformational changes of GPCRs remains unknown. By developing a Förster resonance energy transfer (FRET) tool for human M3 muscarinic receptor (hM3R), we find that a single-receptor FRET probe can display the consecutive structural conversion of a receptor by G protein cycle. Our results reveal that the G protein activation evokes a two-step change in the hM3R structure, including the fast step mediated by Gq protein binding and the subsequent slower step mediated by the physical separation of the Gαq and Gβγ subunits. We also find that the separated Gαq-GTP forms a stable complex with the ligand-activated hM3R and phospholipase Cβ. In sum, the present study uncovers the real-time conformational dynamics of innate hM3R during the downstream Gq protein cycle.
A Shigella flexneri 2a synthetic glycan-based vaccine induces a long-lasting immune response in adults
Authors: Meron-Sudai Shiri , Asato Valeria , Adler Amos , Bialik Anya , Goren Sophy , Ariel-Cohen Ortal , Reizis Arava , Mulard Laurence A. , Phalipon Armelle , Cohen Dani 
Published: March 2023

AbstractShigella is a leading cause of moderate to severe diarrhea worldwide and of diarrhea-associated deaths in children under 5 years of age in low-and middle-income countries. A vaccine against shigellosis is in high demand. SF2a-TT15, a synthetic carbohydrate-based conjugate vaccine candidate against Shigella flexneri 2a (SF2a) was found safe and strongly immunogenic in adult volunteers. Here, SF2a-TT15 at 10 µg oligosaccharide (OS) vaccine dose is shown to induce a sustained immune response in magnitude and functionality in the majority of volunteers followed up 2 and 3 years post-vaccination. High levels of either one of the humoral parameters as well as the number of specific-IgG memory B-cells determined 3 months after vaccination were good predictors of the durability of the immune response. This study is the first to examine the long-term durability of antibody functionality and memory B-cell response induced by a Shigella vaccine candidate.
Annotated computed tomography coronary angiogram images and associated data of normal and diseased arteries
Authors: Gharleghi R. , Adikari D. , Ellenberger K. , Webster M. , Ellis C. , Sowmya A. , Ooi S. , Beier S. 
Published: March 2023

AbstractComputed Tomography Coronary Angiography (CTCA) is a non-invasive method to evaluate coronary artery anatomy and disease. CTCA is ideal for geometry reconstruction to create virtual models of coronary arteries. To our knowledge there is no public dataset that includes centrelines and segmentation of the full coronary tree. We provide anonymized CTCA images, voxel-wise annotations and associated data in the form of centrelines, calcification scores and meshes of the coronary lumen in 20 normal and 20 diseased cases. Images were obtained along with patient information with informed, written consent as part of the Coronary Atlas. Cases were classified as normal (zero calcium score with no signs of stenosis) or diseased (confirmed coronary artery disease). Manual voxel-wise segmentations by three experts were combined using majority voting to generate the final annotations. Provided data can be used for a variety of research purposes, such as 3D printing patient-specific models, development and validation of segmentation algorithms, education and training of medical personnel and in-silico analyses such as testing of medical devices.
Assessment of aerosol persistence in ICUs via low-cost sensor network and zonal models
Authors: Glenn K. , He J. , Rochlin R. , Teng S. , Hecker J. G. , Novosselov I. 
Published: March 2023

AbstractThe COVID-19 pandemic raised public awareness about airborne particulate matter (PM) due to the spread of infectious diseases via the respiratory route. The persistence of potentially infectious aerosols in public spaces and the spread of nosocomial infections in medical settings deserve careful investigation; however, a systematic approach characterizing the fate of aerosols in clinical environments has not been reported. This paper presents a methodology for mapping aerosol propagation using a low-cost PM sensor network in ICU and adjacent environments and the subsequent development of the data-driven zonal model. Mimicking aerosol generation by a patient, we generated trace NaCl aerosols and monitored their propagation in the environment. In positive (closed door) and neutral-pressure (open door) ICUs, up to 6% or 19%, respectively, of all PM escaped through the door gaps; however, the outside sensors did not register an aerosol spike in negative-pressure ICUs. The K-means clustering analysis of temporospatial aerosol concentration data suggests that ICU can be represented by three distinct zones: (1) near the aerosol source, (2) room periphery, and (3) outside the room. The data suggests two-phase plume behavior: dispersion of the original aerosol spike throughout the room, followed by an evacuation phase where "well-mixed" aerosol concentration decayed uniformly. Decay rates were calculated for positive, neutral, and negative pressure operations, with negative-pressure rooms clearing out nearly twice as fast. These decay trends closely followed the air exchange rates. This research demonstrates the methodology for aerosol monitoring in medical settings. This study is limited by a relatively small data set and is specific to single-occupancy ICU rooms. Future work needs to evaluate medical settings with high risks of infectious disease transmission.
Preparation of aluminium-hydroxide-modified diatomite and its fluoride adsorption mechanism
Authors: Xiang Biao , Tang Jiaxi , Feng Xiaojie , Zhu Yongle , Li Yu , Tan Ting 
Published: March 2023

AbstractAs the current excessive accumulation of fluoride (F−) in the environment can be hazardous to human health, it is essential to remove fluoride from wastewater. In this study, diatomite (DA) was used as a raw material and modified using aluminum hydroxide (Al-DA) for use in the adsorption of F− from water bodies. SEM, EDS, XRD, FTIR, and Zeta potential characterization analyses were carried out; adsorption tests and kinetic fitting were performed, and the effects of pH, dosing quantity, and presence of interfering ions on the adsorption of F− by the materials were investigated. The results show that the Freundlich model effectively describes the adsorption process of F− on DA, which therefore involves adsorption-complexation interactions; however, the Langmuir model effectively describes the adsorption process of F− on Al-DA, corresponding to unimolecular layer adsorption mainly via ion-exchange interactions, that is, adsorption is dominated by chemisorption. Aluminum hydroxide was shown to be the main species involved in F− adsorption. The efficiency of F− removal by DA and Al-DA was over 91% and 97% for 2 h, and the adsorption kinetics were effectively fit by the quasi-secondary model, suggesting that chemical interactions between the absorbents and F− control the adsorption process. The adsorption of F− was highly dependent on the pH of the system, and the maximum adsorption performance was obtained at pH 6 and 4. The optimal dosage of DA and Al-DA was 4 g/L. Even in the presence of interfering ions, the removal of F− on Al-DA reached 89%, showing good selectivity. XRD and FTIR studies showed that the mechanism of F− adsorption on Al-DA involved ion exchange and the formation of F–Al bonds.
Neurological outcomes after surgery and postoperative rehabilitation for cervical radiculopathy due to disc disease: a 2-year-follow-up of a randomized clinical trial
Authors: Peolsson Anneli , Löfgren Håkan , Dedering Åsa , Kristedal Mattias , Öberg Birgitta , Zsigmond Peter , Wibault Johanna 
Published: March 2023

AbstractReports on neurological outcomes in patients with cervical radiculopathy (CR) undergoing surgery and postoperative rehabilitation are important to inform prognosis. This 2-year-follow-up of a randomized clinical trial aimed to compare secondary neurological outcomes between structured postoperative rehabilitation and a standard approach after surgery for CR. A secondary aim was to increase knowledge about recovery of neurological impairments in relation to patient-reported neck disability. Neurological outcomes included assessment of sensibility, motor function, arm reflexes and the Spurling test. A total of 153 and 135 participants (> 70% response rate) completed the clinical examination. Between-group differences, changes over time, and associations between persistent neurological impairments and the Neck Disability Index were investigated. No between-group differences were reported (p > 0.07), and neurological impairments in sensibility, motor function, and a positive Spurling test decreased over time in both groups (p < 0.04). Persistent impairments in sensibility and reflex arm were most frequent at follow-up, whereas, a persistent positive Spurling test, and impairments in motor function were associated with higher NDI score. Neurological outcomes improved over time in patients undergoing surgery for CR with no between-group differences., However, persistent neurological impairments were common, and associated with poorer outcome for patient-reported neck disability.Clinical registration: clinicaltrial.gov NCT01547611, 08/03/2012, Title: Outcome of physiotherapy after surgery for cervical disc disease: a prospective multi-centre trial.
Speech and Language Characteristics of Neurologically Healthy Adults When Describing the Modern Cookie Theft Picture: Mixing the New With the Old
Authors: Hux Karen , Frodsham Kayla 
Published: March 2023

Purpose:
The Modern Cookie Theft picture has recently become available. This study's purpose was to compare (a) speech and language production by neurologically healthy adults (NHAs) given a generic instruction to describe the picture versus instruction to describe it as if talking with someone who was blind and (b) production during the first 90 s versus full samples.

Method:
One hundred NHAs minus five outliers formed two participant groups. Each group heard either the original or modified task instruction. Transcriptions of resulting descriptions were analyzed regarding duration, word and T-unit productivity, content units (CUs), and main concepts (MCs) both in full and 90-s samples. Identified CUs and MCs were compared with existing lists from previous research.

Results:
Significantly longer samples and greater verbosity occurred with the modified versus original instruction even when limiting time to a 90 s maximum. With the modified instruction, CUs included 119 and 138 terms for truncated and full samples, respectively; participants mentioned 98 and 104 CUs, respectively, given the original instruction. MCs expressed were 18 and 19 for truncated and full samples, respectively, given the modified instruction; with the original instruction, this dropped to 11 and 12 MCs for truncated and full samples, respectively. Within samples, CU and MC repetitions were greater given modified rather than original instruction.

Conclusions:
Normative productivity and content generation data are critical for guiding diagnostic efforts and treatment planning. Benefits and detriments associated with differing productivity and content redundancy secondary to varying instructions and analysis time frames are discussed.
Sampling rate selection for multi‐loop cascade control systems in an optimal manner
Authors: Şuşcă Mircea , Mihaly Vlad , Dobra Petru 
Published: March 2023

AbstractThis article proposes an optimization‐based approach to selecting the sampling periods for classes of linear time‐invariant (LTI) regulators in a cascaded multi‐rate control structure, to account for practical implementation on numeric systems. Starting from the Shannon‐Nyquist sampling theorem, which proposes the minimum required sampling frequency for completely recovering the original analog signal from its discrete samples, the scope of the sampling process, viewed from a control perspective, is to ensure asymptotic stability of the closed‐loop system, with the smallest possible degradation of the initially‐proposed performance indices as possible according to a set of well‐picked criteria. As such, a configurable optimization‐based approach is proposed, solved with the mixed‐integer artificial bee colony (MI‐ABC) algorithm, for selecting the sampling periods by specifying the emphasis between closed‐loop fidelity and ease of implementation, with an intuitive visualization method. The proposed solution is illustrated on two case studies, with emphasis on the construction of the proposed functionals and their practical implications.
Forward modelling of brightness variations in Sun-like stars
Authors: Nèmec N.-E. , Shapiro A. I. , Işık E. , Solanki S. K. , Reinhold T. 
Published: March 2023

Context. The amplitude and morphology of light curves of Sun-like stars change substantially with increasing rotation rate: brightness variations are amplified and become more regular. This has not been explained so far.
Aims. We develop a modelling approach for calculating brightness variations of stars with various rotation rates and use it to explain the observed trends in stellar photometric variability.
Methods. We combined numerical simulations of magnetic flux emergence and transport with a model for stellar brightness variability to calculate synthetic light curves of stars as observed by the Kepler telescope. We computed the distribution of the magnetic flux on the stellar surface for various rotation rates and degrees of active-region nesting (i.e. the tendency of active regions to emerge in the vicinity of recently emerged regions). Using the resulting maps of the magnetic flux, we computed the rotational variability of our simulated stellar light curves as a function of rotation rate and nesting of magnetic features and compared our calculations to Kepler observations.
Results. We show that both the rotation rate and the degree of nesting have a strong impact on the amplitude and morphology of stellar light curves. In order to explain the variability of most of the Kepler targets with known rotation rates, we need to increase the degree of nesting to values that are much higher than the values on the Sun.
Conclusions. The suggested increase in nesting with the rotation rate can provide clues about the flux emergence process for high levels of stellar activity.
E2F2 Promotes Wound Healing of Diabetic Foot Ulcer by Regulating
CDCA7L Transcription
Authors: Xiao Meimei , Wang Jiusong , Chen Yanming 
Published: March 2023

Abstract
Objective The E2F2 transcription factor can accelerate cell proliferation
and wound healing. However, its mechanism of action in a diabetic foot ulcer
(DFU) remains unclear. Therefore, this study explores the influence of E2F2 on
wound healing in DFU by examining cell division cycle-associated 7-like (CDCA7L)
expression.
Methods CDCA7L and E2F2 expression in DFU tissues were analyzed with
databases. CDCA7L and E2F2 expression were altered in human umbilical vein
endothelial cells (HUVECs) and spontaneously transformed human keratinocyte cell
culture (HaCaT) cells. Cell viability, migration, colony formation, and
angiogenesis were evaluated. Binding of E2F2 to the CDCA7L promoter was
examined. Subsequently, a diabetes mellitus (DM) mouse model was established and
treated with full-thickness excision followed by CDCA7L overexpression. Wound
healing in these mice was observed and recorded, and vascular endothelial growth
factor receptor 2 (VEGFR2) and hematopoietic progenitor cell antigen CD34 (CD34)
expression were determined. E2F2 and CDCA7L expression levels in cells and mice
were evaluated. The expression of growth factors was tested.
Results CDCA7L expression was downregulated in DFU tissues and wound
tissues from DM mice. Mechanistically, E2F2 bound to the CDCA7L promoter to
upregulate CDCA7L expression. E2F2 overexpression enhanced viability, migration,
and growth factor expression in HaCaT cells and HUVECs, and augmented HUVEC
angiogenesis and HaCaT cell proliferation, which was nullified by silencing
CDCA7L. In DM mice, CDCA7L overexpression facilitated wound healing and elevated
the expression level of growth factors.
Conclusions E2F2 facilitated cell proliferation and migration and fostered
wound healing in DFU cells through binding to the CDCA7L promoter.
Die Bauchspeicheldrüse – der Blick fürs
Ganze
Authors: Rudat Rüdiger 
Published: March 2023

ZusammenfassungEine Pathologie der Bauchspeicheldrüse ist vielfach Wegbereiter für
weitere Pathologien desselben Organs. Grund dafür ist die enge
Verknüpfung der endokrinen und exokrinen Anteile sowie die vergleichbaren
Pathogenese-Faktoren der unterschiedlichen Erkrankungen. Phytotherapeutika und
Komplexhomöopathika sind aufgrund ihrer Multi-Target-Wirkungen und der
großen therapeutischen Breite ideale Mittel, um bei Diabetes, Exokriner
Pankreas-Insuffizienz und chronischer Pankreatitis helfend einzugreifen.
Ganzheitliche Diagnose- und Therapieansätze bei Hunden mit
rezidivierender Pankreas-Problematik
Authors: Ailer Marion Nicole 
Published: March 2023

ZusammenfassungRezidivierende Pankreatitiden sind oftmals durch eine falsche
Fütterung bedingt. Dabei scheint weniger die
Fütterungsart an sich, sondern vielmehr ein
unverträglicher Umstand (zu fett, zu schwer verdaulich) der
konkrete Auslöser zu sein. Abhilfe schafft eine strikte
Diät mit nur 50 Prozent Fleischanteil und 50 Prozent gekochtem
Gemüse, zudem sorgt die Zugabe von Pankreasenzymen zur
Vorverdauung des Futters für eine Entlastung der
Bauchspeicheldrüse. Weiterhin können verschiedene
alternative Therapiemethoden unterstützend eingesetzt
werden.
Oxidation Products from the Neolignan Licarin A by Biomimetic Reactions and Assessment of in vivo Acute Toxicity
Authors: Souza Juliana Neves de Paula e , da Silva Rodrigo Moreira , Fortes Simone Silveira , de Oliveira Anderson Rodrigo Moraes , Ferreira Leandro S. , Vessecchi Ricardo , Lopes Norberto Peporine , Silva Denise Brentan 
Published: March 2023

AbstractLicarin A, a dihydrobenzofuranic neolignan presents in several medicinal plants and seeds of nutmeg, exhibits strong activity against protozoans responsible for Chagas disease and
leishmaniasis. From biomimetic reactions by metalloporphyrin and Jacobsen catalysts, seven products were determined: four isomeric products yielded by epoxidation from licarin A, besides a
new product yielded by a vicinal diol, a benzylic aldehyde, and an unsaturated aldehyde in the structure of the licarin A. The incubation with rat and human liver microsomes partially
reproduced the biomimetic reactions by the production of the same epoxidized product of m/z 343 [M + H]+. In vivo acute toxicity assays of licarin A suggested liver
toxicity based on biomarker enzymatic changes. However, microscopic analysis of tissues sections did not show any tissue damage as indicative of toxicity after 14 days of exposure. New
metabolic pathways of the licarin A were identified after in vitro biomimetic oxidation reaction and in vitro metabolism by rat or human liver microsomes.
Kosten potenziell ambulant erbringbarer endoskopischer Leistungen in Fällen mit 1-Tages-Verweildauer gegenüber einer längeren Verweildauer
Authors: Rathmayer Markus , Heinlein Wolfgang , Wagner Tobias , Lerch Markus M. , Lammert Frank , Dollhopf Markus , Haag Cornelie , Gölder Stefan Karl , Kandulski Arne , Schad Moritz , Schmidt Alexandra , Gundling Felix , Wilke Michael , Albert Jörg G. ,   für die DRG-Projektgruppe der DGVS*
Published: March 2023

Zusammenfassung
Einleitung Die „Ambulantisierung“, d.h. die Ausweitung ambulanter Leistungen auf Behandlungen, die bisher stationär erbracht wurden, ist ein erklärtes Ziel der Gesundheitspolitik. Unklar ist, inwieweit Kosten einer endoskopischen Prozedur und die Erkrankungsschwere von der Dauer der stationären Behandlung abhängig sind. Wir überprüften daher, ob endoskopische Leistungen bei Fällen mit einem Tag Verweildauer (VWD) vergleichbar teuer sind wie Fälle mit längerer VWD.
Methodik Aus dem DGVS-Leistungskatalog wurden ambulant erbringbare Leistungen ausgewählt. 1-Tagesfälle mit genau einer solchen gastroenterologisch-endoskopischen (GAEN-)Leistung wurden mit Fällen mit VWD>1 Tag hinsichtlich ihres Patient Clinical Complexity Level (PCCL) und ihrer mittleren Kosten verglichen. Als Datenquelle diente das DGVS-DRG-Projekt mit §21-KHEntgG-Kostendaten von 57 Krankenhäusern aus 2018 und 2019. Endoskopische Kosten wurden der Kostenstellengruppe 8 der InEK-Kostenmatrix entnommen und plausibilisiert.
Ergebnisse Insgesamt wurden 122.514 Fälle mit genau einer GAEN-Leistung identifiziert. In 30 von 47 Leistungsgruppen zeigten sich statistisch gleiche Kosten. In 10 Gruppen war der Kostenunterschied nicht relevant (<10%). Kostenunterschiede >10% bestanden bei der ÖGD mit Varizentherapie, Einlage einer selbstexpandierenden Prothese, Dilatation / Bougierung, dem Wechsel einer PTCD, der nicht aufwendigen ERCP, der Endosonografie sowie der Koloskopie mit endoskopischer Mukosaresektion, endoskopischer Vollwandresektion oder Fremdkörperentfernung. Der PCCL-Wert war in allen bis auf einen Fall unterschiedlich.
Schlussfolgerung GAEN-Leistungen, die stationär erbracht wurden und potenziell ambulant erbringbar sind, sind bei 1-Tagesfällen überwiegend gleich teuer wie bei Patienten mit einer VWD von mehr als einem Tag. Die Erkrankungsschwere ist geringer. Kalkulierte §21-KHEntgG-Kostendaten bilden damit eine belastbare Basis für die Kalkulation einer sachgerechten Vergütung von zukünftig im Rahmen des AOP ambulant zu erbringenden Leistungen im Krankenhaus.