bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics of comorbid tic disorders in autism spectrum disorder: exploratory analysis
Authors: Kim Ye Rim , Song Da-Yea , Bong Guiyoung , Han Jae Hyun , Kim Joo-Hyun , Yoo Hee Jeong 
Published: June 2023

Abstract
Background
The frequency, clinical characteristics, and associated symptoms of comorbid tic disorders in individuals with autism spectrum disorder (ASD) remain unclear.

Methods
We included subsets of individuals from a larger genetic study who were diagnosed with ASD (n = 679; age: 4–18 years) and completed the Yale Global Tic Severity Scale (YGTSS) questionnaire. Based on the YGTSS score, the individuals were divided into two groups: ASD only (n = 554) and ASD with tics (n = 125). Individuals were assessed using the verbal and non-verbal intelligence quotient (IQ), Vineland Adaptive Behavior Scale (VABS-2), Social Responsiveness Scale-2 (SRS-2), Child Behavior Checklists (CBCL), and Yale-Brown Obsessive–Compulsive Scale (YBOCS), followed by between-group comparisons. All statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS) version 26.

Results
Tic symptoms were observed in 125 (18.4%) participants; among them, most participants presented both motor and vocal tics (n = 40, 40.0%). The ASD with tics group had a significantly higher average age and full-scale IQ score than the ASD only group. After adjusting for age, the ASD with tics group had significantly higher scores in the SRS-2, CBCL, and YBOCS subdomains than the ASD only group. Furthermore, all variables except the non-verbal IQ and VABS-2 scores were positively correlated with the YGTSS total score. Finally, the proportion of tic symptoms was significantly higher among individuals with a higher IQ score (≥ 70).

Conclusions
The IQ score was positively correlated with the proportion of tic symptoms among individuals with ASD. Moreover, the severity of the core and comorbid symptoms of ASD was associated with the occurrence and severity of tic disorders. Our findings suggest the need for appropriate clinical interventions for individuals with ASD.
Trial registration This study retrospectively registered participants
Nine golden codes: improving the accuracy of Helicopter Emergency Medical Services (HEMS) dispatch—a retrospective, multi-organisational study in the East of England
Authors: Edmunds Christopher T. , Lachowycz Kate , McLachlan Sarah , Downes Andrew , Smith Andrew , Major Rob , Barnard Edward B. G. 
Published: June 2023

Abstract
Background
Helicopter Emergency Medical Services (HEMS) are a limited and expensive resource, and should be intelligently tasked. HEMS dispatch was identified as a key research priority in 2011, with a call to identify a ‘general set of criteria with the highest discriminating potential’. However, there have been no published data analyses in the past decade that specifically address this priority, and this priority has been reaffirmed in 2023. The objective of this study was to define the dispatch criteria available at the time of the initial emergency call with the greatest HEMS utility using a large, regional, multi-organizational dataset in the UK.

Methods
This retrospective observational study utilized dispatch data from a regional emergency medical service (EMS) and three HEMS organisations in the East of England, 2016–2019. In a logistic regression model, Advanced Medical Priority Dispatch System (AMPDS) codes with ≥ 50 HEMS dispatches in the study period were compared with the remainder to identify codes with high-levels of HEMS patient contact and HEMS-level intervention/drug/diagnostic (HLIDD). The primary outcome was to identify AMPDS codes with a > 10% HEMS dispatch rate of all EMS taskings that would result in 10–20 high-utility HEMS dispatches per 24-h period in the East of England. Data were analysed in R, and are reported as number (percentage); significance was p < 0.05.

Results
There were n = 25,491 HEMS dispatches (6400 per year), of which n = 23,030 (90.3%) had an associated AMPDS code. n = 13,778 (59.8%) of HEMS dispatches resulted in patient contact, and n = 8437 (36.6%) had an HLIDD. 43 AMPDS codes had significantly greater rates of patient contact and/or HLIDD compared to the reference group. In an exploratory analysis, a cut-off of ≥ 70% patient contact rate and/or ≥ 70% HLIDD (with a > 10% HEMS dispatch of all EMS taskings) resulted in 17 taskings per 24-h period. This definition derived nine AMPDS codes with high HEMS utility.

Conclusion
We have identified nine ‘golden’ AMPDS codes, available at the time of initial emergency call, that are associated with high-levels of whole-system and HEMS utility in the East of England. We propose that UK EMS should consider immediate HEMS dispatch to these codes.
16S rRNA gene sequencing of stool samples collected from patients with latent tuberculosis infection before, during, and two months after treatment with 3HP or 4R
Authors: Séraphin Marie Nancy , Bellot Julia , Peloquin Charles A. , Mai Volker 
Published: June 2023

Abstract
Objective
We present 16s rRNA gene sequencing (V1-V2 region) and sample data from a pilot observational cohort study to describe the gut microbiota dynamics of subjects with latent tuberculosis infection (LTBI) treated with daily 600 mg rifampicin for four months (4R) or a weekly dose of 900 mg combination of rifapentine and isoniazid for three months (3HP). Our objectives were to (1) document changes to the gut microbiota immediately following exposure to the rifamycins and (2) document recovery to baseline two months after treatment completion.

Data description
We enrolled six subjects with subjects with LTBI and prospectively followed them for 5–6 months. Each subject provided stool samples before, during, and two months after treatment. Six healthy controls were sampled in parallel with the patients with LTBIs. We report amplicon sequence variants (ASVs) and taxonomic assignments for 60 stool samples. Additionally, we provide access to the raw amplicon sequences, and subject responses to questionnaires about their diet, medication, and lifestyle changes over the study follow-up period. Furthermore, we provide the concentration of the parent and partially active rifamycin metabolite concentrations measured validated LC-MS-MS assays of phosphate buffer washes of the stool samples collected from the LTBI participants. This comprehensive dataset is a valuable resource for future systematic reviews and meta-analyses of the impact of LTBI therapy on the gut microbiota.
SETD8, a frequently mutated gene in cervical cancer, enhances cisplatin sensitivity by impairing DNA repair
Authors: Wang Xin , Cao Chen , Tan Xiangyu , Liao Xueyao , Du Xiaofang , Wang Xueqian , Liu Ting , Gong Danni , Hu Zheng , Tian Xun 
Published: June 2023

Abstract
Background
Cisplatin is commonly used to treat cervical cancer while drug resistance limits its effectiveness. There is an urgent need to identify strategies that increase cisplatin sensitivity and improve the outcomes of chemotherapy.

Results
We performed whole exome sequencing (WES) of 156 cervical cancer tissues to assess genomic features related to platinum-based chemoresistance. By using WES, we identified a frequently mutated locus SETD8 (7%), which was associated with drug sensitivity. Cell functional assays, in vivo xenografts tumor growth experiments, and survival analysis were used to investigate the functional significance and mechanism of chemosensitization after SETD8 downregulation. Knockdown of SETD8 increased the responsiveness of cervical cancer cells to cisplatin treatment. The mechanism is exerted by reduced binding of 53BP1 to DNA breaks and inhibition of the non-homologous end joining (NHEJ) repair pathway. In addition, SETD8 expression was positively correlated with resistance to cisplatin and negatively associated with the prognosis of cervical cancer patients. Further, UNC0379 as a small molecule inhibitor of SETD8 was found to enhance cisplatin sensitivity both in vitro and in vivo.

Conclusions
SETD8 was a promising therapeutic target to ameliorate cisplatin resistance and improve the efficacy of chemotherapy.
Ahnak is required to balance calcium ion homeostasis and smooth muscle development in the urinary system
Authors: Lee Jong-Min , Lim Tae-Yang , Oh Sang-Bin , Lee Seung-Jun , Bae Yun Soo , Jung Han-Sung 
Published: June 2023

Abstract
Background
Various renal abnormalities, including hydronephrosis, polycystic kidney disease, and hydroureter, have been reported, and these abnormalities are present in DiGeorge syndrome, renal dysplasia, and acute kidney failure. Previous studies have shown that various genes are associated with renal abnormalities. However, the major target genes of nonobstructive hydronephrosis have not yet been elucidated.

Results
We examined neuroblast differentiation-associated protein Ahnak localization and analyzed morphogenesis in developing kidney and ureter. To investigated function of Ahnak, RNA-sequencing and calcium imaging were performed in wild type and Ahnak knockout (KO) mice. Ahnak localization was confirmed in the developing mouse kidneys and ureter. An imbalance of calcium homeostasis and hydronephrosis, which involves an expanded renal pelvis and hydroureter, was observed in Ahnak KO mice. Gene Ontology enrichment analysis on RNA-seq results indicated that ‘Channel Activity’, ‘Passive Transmembrane Transporter Activity’ and ‘Cellular Calcium Ion Homeostasis’ were downregulated in Ahnak KO kidney. ‘Muscle Tissue Development’, ‘Muscle Contraction’, and ‘Cellular Calcium Ion Homeostasis’ were downregulated in Ahnak KO ureter. Moreover, peristaltic movement of smooth muscle in the ureter was reduced in Ahnak KO mice.

Conclusions
Abnormal calcium homeostasis causes renal disease and is regulated by calcium channels. In this study, we focused on Ahnak, which regulates calcium homeostasis in several organs. Our results indicate that Ahnak plays a pivotal role in kidney and ureter development, and in maintaining the function of the urinary system.
Biological quality and phytochemical profiling of olive fruits using gas chromatography–mass spectrometry (GCMS) analysis
Authors: Ahmad Rizwan , Alqathama Aljawharah , Alam Mohammad Mahtab , Riaz Muhammad , Abdalla Ashraf N. , Aldholmi Mohammed , Al− Said Hamdi M. , Aljishi Fatema S. , Althomali Ebtihal H. , Alabdullah Murtada M. , Altaweel Nezar H. , Almubarak Ali F. , Asghar Sami. S. 
Published: June 2023

AbstractThe quality of olive fruit (OF) is widely affected due to geographical variation, affecting OF's chemical composition and biological properties. It is a novel and first-time study to evaluate the quality variation of 42 olive samples from different geographical origins based on phytochemical profile and their biological activities. The study reports the presence of unique chemical markers responsible for the difference in quality and biological activity of the olive samples. Biological activity (cytotoxic and antimicrobial) with GCMS phytochemical profile was evaluated. GCMS analysis confirmed the presence of 111 volatile compounds from various chemical classes with range (%) and average (%): esters (21.61–60.49) and 44.62, alcohols (20.73–49.2) and 38.06, hydrocarbons (3–38.88) and 15.39, ketones (0.16–3.87) and 0.75, acids (0.07–2.62) and 0.27, and aldehydes (0.12–1.47) and 0.45. The predominant ester was 13-methyl-pentadecanoic acid methyl ester, a differentiation marker between these samples. Cytotoxicity assay showed a significant inhibitory effect against MCF7 (8–64%) and HCT116 (0.11–44%) cell lines, whereas the extracts with the highest cytotoxicity observed were O17 (52.00 ± 2.00) and O25 (64.00 ± 4.88). The antimicrobial activity exhibited a range of zones of inhibition (mm) againstP. aeruginosa(0.00–17.00),E. coli(0.00–15.00),S. aureus(0.00–13), and resistantS. aureus,i.e., MRSA (0.00–12.00). The extracts with the highest antimicrobial activity, i.e., O8 and O39 had identical MIC and MBC of 12.5 and 25 µg/ml againstP. aeruginosa. In contrast, an MIC (50 and 25) and MBC (100 and 50) againstE. coliwere determined for O39 and O8. The statistical PCA and K-mean cluster analysis (P < 0.05) confirmed the presence of a high number of esters, alcohols, and hydrocarbons in GCMS data. Moreover, O8, O23, O25, and O39 were suggested as comparatively better varieties than those OF samples (P = 0.001). The presence of distinct volatile markers in these 42 OF samples may be further studied as a potential source of antimicrobials, food preservatives and therapeutic purposes.Graphical abstract
Literature and Totality
Authors: Ansari Alya 
Published: June 2023

Abstract
This essay foregrounds the hermeneutic purchase of totality in contemporary literary criticism. Responding to the recent proliferation of the “gig work” novel, the essay takes up two interrelated lines of inquiry: How might we rethink the conceptual affordances of “totality” for the ongoing project of the critique of political economy? What would a rethinking of totality’s position in the conceptual architecture of literary criticism offer in the way of new heuristics for the analysis of the novel? Through recourse to G. W. F. Hegel’s Science of Logic and Michael Theunissen, Hans-Friedrich Fulda, and Rolf-Peter Horstmann’s Critical Presentation of Metaphysics: A Discussion of Hegel’s “Logic” (Kritische Darstellung der Metaphysik: Eine Diskussion über Hegels “Logik”), this essay proposes a method of literary analysis that approaches the formal aspects of the novel as defined through the historical-material conditions for the writing of the text. The essay then puts a close reading of Hilary Leichter’s Temporary in conversation with Sarah Brouillette’s account of the decline of the English-language literary novel to suggest how the formal properties of the contemporary gig work novel respond to the general crisis of novel production in the twenty-first century.
A novel porcine model of CLN3 Batten disease recapitulates clinical phenotypes
Authors: Swier Vicki J. , White Katherine A. , Johnson Tyler B. , Wang Xiaojun , Han Jimin , Pearce David A. , Singh Ruchira , Drack Arlene V. , Pfeifer Wanda , Rogers Christopher S. , Brudvig Jon J. , Weimer Jill M. 
Published: June 2023

ABSTRACTMouse models of CLN3 Batten disease, a rare lysosomal storage disorder with no cure, have improved our understanding of CLN3 biology and therapeutics through their ease of use and a consistent display of cellular pathology. However, the translatability of murine models is limited by disparities in anatomy, body size, life span and inconsistent subtle behavior deficits that can be difficult to detect in CLN3 mutant mouse models, thereby limiting their use in preclinical studies. Here, we present a longitudinal characterization of a novel miniswine model of CLN3 disease that recapitulates the most common human pathogenic variant, an exon 7-8 deletion (CLN3Δex7/8). Progressive pathology and neuron loss is observed in various regions of the CLN3Δex7/8 miniswine brain and retina. Additionally, mutant miniswine present with retinal degeneration and motor abnormalities, similar to deficits seen in humans diagnosed with the disease. Taken together, the CLN3Δex7/8 miniswine model shows consistent and progressive Batten disease pathology, and behavioral impairment mirroring clinical presentation, demonstrating its value in studying the role of CLN3 and safety/efficacy of novel disease-modifying therapeutics.
Different tether proteins of the same membrane contact site affect the localization and mobility of each other
Authors: Amado Lucia , Cogan Alexandra P. , González Montoro Ayelén 
Published: June 2023

ABSTRACT
Membrane contact sites enable the exchange of metabolites between subcellular compartments and regulate organelle dynamics and positioning. These structures often contain multiple proteins that tether the membranes, establishing the apposition and functionalizing the structure. In this work, we used drug-inducible tethers in vivo in Saccharomyces cerevisiae to address how different tethers influence each other. We found that the establishment of a region of membrane proximity can recruit tethers, influencing their distribution between different locations or protein complexes. In addition, restricting the localization of one tether to a subdomain of an organelle caused other tethers to be restricted there. Finally, we show that the mobility of contact site tethers can also be influenced by other tethers of the same interface. Overall, our results show that the presence of other tethers at contact sites is an important determinant of the behavior of tethering proteins. This suggests that contact sites with multiple tethers are controlled by the interplay between specific molecular interactions and the cross-influence of tethers of the same interface.
A Dynamic Ankle Orthosis Reduces Tibial Compressive Force and Increases Ankle Motion Compared With a Walking Boot
Authors: JOHNSON PERRI A. , PAQUETTE MAX R. , DIANGELO DENIS J. 
Published: June 2023

ABSTRACT

Purpose
Tibial bone stress injuries are a common overuse injury among runners and military cadets. Current treatment involves wearing an orthopedic walking boot for 3 to 12 wk, which limits ankle motion and leads to lower limb muscle atrophy. A dynamic ankle orthosis (DAO) was designed to provide a distractive force that offloads in-shoe vertical force and retains sagittal ankle motion during walking. It remains unclear how tibial compressive force is altered by the DAO. This study compared tibial compressive force and ankle motion during walking between the DAO and an orthopedic walking boot.

Methods
Twenty young adults walked on an instrumented treadmill at 1.0 m·s−1 in two brace conditions: DAO and walking boot. Three-dimensional kinematic, ground reaction forces, and in-shoe vertical force data were collected to calculate peak tibial compressive force. Paired t-tests and Cohen’s d effect sizes were used to assess mean differences between conditions.

Results
Peak tibial compressive force (P = 0.023; d = 0.5) and Achilles tendon force (P = 0.017; d = 0.5) were moderately lower in the DAO compared with the walking boot. Sagittal ankle excursion was 54.9% greater in the DAO compared with the walking boot (P = 0.05; d = 3.1).

Conclusions
The findings from this study indicated that the DAO moderately reduced tibial compressive force and Achilles tendon force and allowed more sagittal ankle excursion during treadmill walking compared with an orthopedic walking boot.
The role of human property administration in quality management in the public sector
Authors: Gogalis Theodoros 
Published: June 2023

Human Resource Management is defined as "The design, implementation and maintenance of strategies to manage people for optimum organization performance where they work". It constitutes a set of strategies to be implemented in the public sector so that its operations could give high quality services and satisfaction to the citizens.
This paper deals with the role and importance of human resource management in creating an efficient and functionally sound Greek public sector. An attempt is made to analyze the importance of Human Resource Management in the operation and development of public bodies through its connection to the Common Evaluation Framework which is essentially a "new" tool for Total Quality Management and which has been the subject of research by many scholars.
It is apparent the lack of adoption of quality management principles and the lack of a culture of change which leads to reduced efficiency of public services and its human resources. This combined by the lack of implementation of modern Total Quality Management practices with due emphasis on skills, employee training, performance appraisal, communication and labor relations.
The present research has shown that it is imperative to upgrade the human resource management strategies implemented in the public sector of our country through the broader and more effective implementation of the principles of human resource management in order to both enhance employee performance and effective functioning of the public sector.
Keywords: human resources management, total quality management, management practices, public sector.
A project on “wine and distillation trade” course. The consumers mindset regarding retsina
Authors: Iliadou Georgia , Giannopoulos Dionisis , Nikolakakis Konstantinos 
Published: June 2023

On early ‘70s the consumers stopped preferring retsina, due to many reasons, some of them have already been mentioned, like the bad quality of the wine, that the owners of small Greek restaurants used and with the use of resin they were trying to cover the wine’s flaws, of the change of the lifestyle, because anything that was related to the tradition or the countryside was characterized as outdated. Finally, the living standards change and that made the consumers seek of more elegant and aromatic and wines. The last decade despite all the above facts, some wine producers have started to produce higher quality of resinated wine to ensure that this product will remain on the market. The cause of this research is to figure out what is today’s consumers mindset regarding resonated wine and their opinion on the new trend of higher quality retsina.
The method which used, was to collect data through a questionnaire. The questions are about demographics, questions about wine consumption and questions about retsina. The questionnaire was shared through Social Media. The number of answers reached 768 questionnaires throughout Greece. The results were processed with SPSS Statistics 22.

Keywords: marketing, resinated, wine, Retsina, SPSS analysis
JEL Classification Code: M31, L36
Branding commodity, tourist & cultural products: some thoughts on applying semiotic analysis for the marketing strategy in each product category
Authors: Papadaki Eirini 
Published: June 2023

This paper proposes the adoption of semiotic analysis to the branding process in an attempt to understand the function of the specific sign system and successfully manage its designing and evaluation processes, from audience research to audience targeting. Specific case studies of commodity, tourist and cultural product branding will be presented, in an attempt to underline, in a parallel goal, the differences and similarities of the symbolic process involved in the three areas: commodities, tourism and culture.

EL Classifications: M, Z
Key words: branding, commodity products, cultural products, tourist products, semiotics
A Direct Utility Model for Access Costs and Economies of Scope
Authors: Kim Dong Soo , Lee Sanghak , Hur Taegyu , Kim Jaehwan , Allenby Greg M. 
Published: June 2023

Consumers encounter costs and inconvenience in the purchase and consumption of goods. Time, effort, training, and expertise, also referred to as access costs, are examples of factors that constrain the ability of consumers to derive utility from offerings beyond the price of a good. Economies of scope exist when household production of these factors share commonalities among choice alternatives, making it more likely that some products would be purchased and consumed in conjunction with others. For example, consumers planning out-of-town travel might consider visiting multiple tourist attractions during their trip, and course offerings might appeal more to students who are already in the mindset of taking courses. We develop a model with economies of scope that captures a leveraging effect where the purchase of one good makes the purchase of other goods more likely. Our model offers an alternative explanation of complementary purchases that are driven by cost reductions rather than utility enhancements. The model is applied to four conjoint datasets where we find that economies of scope and access costs play an important role in consumer purchases and have high economic value relative to the role played by price. This paper was accepted by Eric Anderson, marketing. Supplemental Material: The data files and online appendices are available at https://doi.org/10.1287/mnsc.2023.4841 .