bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the cellular and molecular differences between ovarian clear cell carcinoma and high-grade serous carcinoma using single-cell RNA sequencing and GEO gene expression signatures
Authors: Guo Dan , Zhang Sumei , Gao Yike , Shi Jinghua , Wang Xiaoxi , Zhang Zixin , Zhang Yaran , Wang Yuming , Zhao Kun , Li Mei , Wang Anqi , Wang Pan , Gou Yanqin , Zhang Miao , Liu Meiyu , Zhang Yuhan , Chen Rui , Sun Jian , Wang Shu , Wu Xunyao , Liang Zhiyong , Chen Jie , Lang Jinghe 
Published: July 2023

AbstractThe two most prevalent subtypes of epithelial ovarian carcinoma (EOC) are ovarian clear cell carcinoma (OCCC) and high-grade serous ovarian carcinoma (HGSC). Patients with OCCC have a poor prognosis than those with HGSC due to chemoresistance, implying the need for novel treatment target. In this study, we applied single-cell RNA sequencing (scRNA-seq) together with bulk RNA-seq data from the GEO (Gene Expression Omnibus) database (the GSE189553 dataset) to characterize and compare tumor heterogeneity and cell-level evolution between OCCC and HGSC samples. To begin, we found that the smaller proportion of an epithelial OCCC cell subset in the G2/M phase might explain OCCC chemoresistance. Second, we identified a possible pathogenic OCCC epithelial cell subcluster that overexpresses LEFTY1. Third, novel biomarkers separating OCCC from HGSC were discovered and subsequently validated on a wide scale using immunohistochemistry. Amine oxidase copper containing 1 (AOC1) was preferentially expressed in OCCC over HGSC, while S100 calcium-binding protein A2 (S100A2) was detected less frequently in OCCC than in HGSC. In addition, we discovered that metabolic pathways were enriched in the epithelial compartment of the OCCC samples. In vitro experiments verified that inhibition of oxidative phosphorylation or glycolysis pathways exerted direct antitumor effects on both OCCC and HGSC cells, while targeting glutamine metabolism or ferroptosis greatly attenuated chemosensitivity only in OCCC cells. Finally, to determine whether there were any variations in immune cell subsets between OCCC and HGSC, data from scRNA-seq and mass cytometry were pooled for analysis. In summary, our work provides the first holistic insights into the cellular and molecular distinctions between OCCC and HGSC and is a valuable source for discovering new targets to leverage in clinical treatments to improve the poor prognosis of patients with OCCC.
Experimental and Numerical Analysis of the Ribbed Reinforced Concrete Fracture Behavior Based on the Mesoscale FE Model
Authors: Wang Zhanliang , Zhang Wei , Huang Yiqun 
Published: July 2023

AbstractThis study presented a meso-model for the fracture analysis of the reinforced concrete (RC) structure. A modeling method of RC meso-structure was proposed, and the rebars were allowed to separate from the concrete. The model was built using the cohesive zone model (CZM). The zero-thickness cohesive elements were adopted to characterize the mechanical behavior of potential fracture surfaces and rebar–concrete interfaces. The constitutive model for concrete was developed by considering the damage relation and friction effect, and the corresponding constitutive for the rebar–concrete interface (especially ribbed rebar) was developed by considering the influence of normal separation on the tangential bond–slip relation. To validate the proposed meso-model, a series of ribbed RC beams with an initial notch was designed and tested by four-point bending loading to obtain different fracture patterns. Through comparison, the developed RC meso-model was validated to simulate the RC structure's fracture behavior appropriately. The influence of the rebar–concrete interface constitutive model on the simulation results was investigated. The investigation results indicate that neglecting normal separation would result in an overestimation of the structure's stiffness and bearing capacity (the peak load was overestimated by more than 10%). Finally, an analysis was conducted on the energy consumption during the failure process of the RC beams. It was found that the proportion of energy consumption during tensile failure of the beam decreased from approximately 86% to 89% in the early stage to approximately 43% to 52% in the later stage, indicating a transition in the beam's failure mode from tensile failure to shear failure.
Transcriptome and proteome analysis reveals the anti-cancer properties of Hypnea musciformis marine macroalga extract in liver and intestinal cancer cells
Authors: Begolli Rodiola , Chatziangelou Myrto , Samiotaki Martina , Goutas Andreas , Barda Sofia , Goutzourelas Nikolaos , Kevrekidis Dimitrios Phaedon , Malea Paraskevi , Trachana Varvara , Liu Ming , Lin Xiukun , Kollatos Nikolaos , Stagos Dimitrios , Giakountis Antonis 
Published: July 2023

Abstract
Background
Marine seaweeds are considered as a rich source of health-promoting compounds by the food and pharmaceutical industry. Hypnea musciformis is a marine red macroalga (seaweed) that is widely distributed throughout the world, including the Mediterranean Sea. It is known to contain various bioactive compounds, including sulfated polysaccharides, flavonoids, and phlorotannins. Recent studies have investigated the potential anticancer effects of extracts from H. musciformis demonstrating their cytotoxic effects on various cancer cell lines. The anticancer effects of these extracts are thought to be due to the presence of bioactive compounds, particularly sulfated polysaccharides, which have been shown to have anticancer and immunomodulatory effects. However, further studies are needed to fully understand the molecular mechanisms that underlie their anticancer effects and to determine their potential as therapeutic agents for cancer treatment.

Methods
H. musciformis was collected from the Aegean Sea (Greece) and used for extract preparation. Transcriptome and proteome analysis was performed in liver and colon cancer human cell lines following treatment with H. musciformis seaweed extracts to characterize its anticancer effect in detail at the molecular level and to link transcriptome and proteome responses to the observed phenotypes in cancer cells.

Results
We have identified that treatment with the seaweed extract triggers a p53-mediated response at the transcriptional and protein level in liver cancer cells, in contrast to colon cancer cells in which the effects are more associated with metabolic changes. Furthermore, we show that in treated HepG2 liver cancer cells, p53 interacts with the chromatin of several target genes and facilitates their upregulation possibly through the recruitment of the p300 co-activator.

Conclusions
Overall, the available evidence suggests that extracts from H. musciformis have the potential to serve as a source of anticancer agents in liver cancer cells mainly through activation of a p53-mediated anti-tumor response that is linked to inhibition of cellular proliferation and induction of cell death.
Sodium Bicarbonate and Time-to-Exhaustion Cycling Performance: A Retrospective Analysis Exploring the Mediating Role of Expectation
Authors: Gurton William H. , Matta Guilherme G. , Gough Lewis Anthony , Ranchordas Mayur Krachna , King David G. , Hurst Philip 
Published: July 2023

Abstract
Background
Research has shown that ingesting 0.3 g·kg−1 body mass sodium bicarbonate (NaHCO3) can improve time-to-exhaustion (TTE) cycling performance, but the influence of psychophysiological mechanisms on ergogenic effects is not yet understood.

Objective
This study retrospectively examined whether changes in TTE cycling performance are mediated by positive expectations of receiving NaHCO3 and/or the decline in blood bicarbonate during exercise.

Methods
In a randomised, crossover, counterbalanced, double-blind, placebo-controlled design, 12 recreationally trained cyclists (maximal oxygen consumption, 54.4 ± 5.7 mL·kg·min−1) performed four TTE cycling tests 90 min after consuming: (1) 0.3 g·kg−1 body mass NaHCO3 in 5 mL·kg−1 body mass solution, (2) 0.03 g·kg−1 body mass sodium chloride in solution (placebo), (3) 0.3 g·kg−1 body mass NaHCO3 in capsules and (4) cornflour in capsules (placebo). Prior to exercise, participants rated on 1–5 Likert type scales how much they expected the treatment they believe had been given would improve performance. Capillary blood samples were measured for acid-base balance at baseline, pre-exercise and post-exercise.

Results
Administering NaHCO3 in solution and capsules improved TTE compared with their respective placebos (solution: 27.0 ± 21.9 s, p = 0.001; capsules: 23.0 ± 28.1 s, p = 0.016). Compared to capsules, NaHCO3 administered via solution resulted in a higher expectancy about the benefits on TTE cycling performance (Median: 3.5 vs. 2.5, Z = 2.135, p = 0.033). Decline in blood bicarbonate during exercise was higher for NaHCO3 given in solution compared to capsules (2.7 ± 2.1 mmol·L−1, p = 0.001). Mediation analyses showed that improvements in TTE cycling were indirectly related to expectancy and decline in blood bicarbonate when NaHCO3 was administered in solution but not capsules.

Conclusions
Participants’ higher expectations when NaHCO3 is administered in solution could result in them exerting themselves harder during TTE cycling, which subsequently leads to a greater decline in blood bicarbonate and larger improvements in performance.

Key Points

Ingesting 0.3 g·kg−1 body mass sodium bicarbonate in solution and capsules improved time-to-exhaustion cycling performance

Positive expectancy about the benefits of sodium bicarbonate and decline in blood bicarbonate were higher when sodium bicarbonate was administered in solution compared with capsules

Improvements in time-to-exhaustion cycling performance for sodium bicarbonate administered in solution were related to expectancy and the enhanced extracellular buffering response
The development of adult T cell leukemia/lymphoma in renal transplant recipients: report of two cases with literature review
Authors: Kawano Noriaki , Kyohei Yamada , Miyoshi Hiroaki , Yoshida Noriaki , Ohshima Kouichi , Arakawa Fumiko , Nakashima Kazutaka , Kameda Takuro , Kogure Yasunori , Ito Yuta , Yoshida Shuro , Kuriyama Takuro , Nakaike Takashi , Tochigi Taro , Takigawa Ken , Yamashita Kiyoshi , Toyofuku Atsushi , Manabe Tatsuya , Doi Atsushi , Terasaka Soushi , Marutsuka Kouske , Ochiai Hidenobu , Kikuchi Ikuo , Mori Yasuo , Kataoka Keisuke , Yoshizumi Tomoharu , Yamauchi Junji , Yamano Yoshihisa , Shimoda Kazuya 
Published: July 2023

Abstract
Backgrounds
Therefore, reports on the risk of HTLV-1-related diseases in organ transplantation have increased in recent years, and the management of HTLV in renal transplantation remains a challenge.

Patients and methods
We retrospectively analyzed four HTLV-1-positive recipients or donors among 89 renal transplantation cases from 2006 to 2021.

Results
Among the four HTLV-1-positive recipients, two patients developed adult T cell leukemia/lymphoma (ATL) derived from recipients at approximately 3 years (1016 days and 1195 days) after renal transplantation. Case 1 developed lymphoma-type ATL (an extranodal primary cutaneous variant), including skin and pulmonary lesions. The patient achieved CR with FK tapering and CHOP therapy following cord blood stem cell transplantation. However, the patient died 101 days after ATL development because of a severe fungal infection. Case 2 developed acute-type ATL with an unusual phenotype of CD4+8+30+. The patient was treated with FK tapering and palliative therapy because of poor PS. Notably, in case 1, histopathological findings showed high numbers of PD-1-positive TIL cells in ATL, suggesting exhausted T cells and a correlation with the early onset of ATL. Furthermore, in Case 2, histopathological findings revealed CD 30 expression in ATL cells, suggesting the importance of CD 30 in ATL development. Importantly, case 2 showed typical driver mutations, including CCR4 truncation mutations of the C-terminal, TBL1XR1 mutation, and TP53 mutation in the splice site. Notably, our present study and our previous study on renal transplantation strongly indicated that two out of two and one out of 59 “recipient” positive cases developed ATL, respectively. Furthermore, our previous nationwide study 4 out of 10 “donor” positive cases developed HAM. These findings showed that ATL may be derived from HTLV-I+ recipient cells and HAM may be derived from HTLV-1+ donor cells, although the precise mechanism remains unknown.

Conclusions
Thus, early onset and rapid progression of ATL with poor outcomes should be considered in HTLV-1 endemic areas. Furthermore, immunological or genetic mechanisms may be related to the development of ATL after renal transplantation. We believe that the mechanism of onset of ATL after transplantation may be important when considering the immune environment of ATL itself.
Post-traumatic meningoencephalocele as a complication after head trauma and surgery: literature review focusing on the relevance of patient’s history and radiological follow-up
Authors: Pesce Alessandro , Armocida Daniele , Petrella Gianpaolo , Frati Alessandro , Pompucci Angelo 
Published: July 2023

Abstract
Background
Meningoencephalocele (ME) is an herniation of brain parenchyma covered by meninges through a bone defect and could be malformative or secondary. Except for rarer cases of spontaneous form, ME is usually due to endonasal or otologic infections and rarely after head trauma. In predisposed patients, even mild head trauma can lead to the formation of a ME.

Methods
We performed a systematic review of literature with the aims to identify the clinical characteristics of all reported forms of post-traumatic ME and the best diagnostic and treatment strategy. We illustrated a case of a patient treated for a post-traumatic subdural hematoma who developed cerebrospinal fluid leakage 3 months after the trauma.

Results
The search returned a total of 59 papers for the analysis, including radiological, clinical studies, technical note and the case reported from our experience. The total number of patients collected for this review was 61, with a mean age of 31.1 years. The diagnosis of ME could be heterogeneous in terms of timing and clinical onset after a head injury. Symptoms onset and subsequent radiological diagnosis of ME vary between 24 h to 43 years. The majority of traumas were reported in temporal site (52.45%). There were reported high variability of treatment strategies dependent on the location and extent of the defect: in the majority of cases (58%), duroplasty by the heterologous dural patch was the procedure of choice. There is a relative low rate of complications (6.5%) due to a delayed diagnosis of ME.

Conclusions
When ME is associated with violation of meninges, the clinical presentation may be that of cerebrospinal fluid otorrhoea or otorhinorrhoea, consequently, delay in diagnosis can lead to neurological complications. The clinical effectiveness of ME treatment depends much more on the correct and timely diagnosis than on the type of procedure selected.
The COVID-19 pandemic and cannabis use in Canada―a scoping review
Authors: Newport Kelda , Bishop Lisa , Donnan Jennifer , Pal Shefali , Najafizada Maisam 
Published: July 2023

Abstract
Background
Since the start of the COVID-19 pandemic in Canada, the cannabis industry has adapted to public health emergency orders which had direct and indirect consequences on cannabis consumption. The objective of this scoping review was to describe the patterns of consumption and cannabis-related health and safety considerations during the COVID-19 pandemic in Canada.

Methods
For this scoping review, we searched four electronic databases supplemented with grey literature. Peer-reviewed or pre-print studies using any study design and grey literature reporting real-world data were included if published in English between March 2020 and September 2021 and focused on cannabis and COVID-19 in Canada. A content analysis was performed.

Results
Twenty-one studies met the inclusion/exclusion criteria. Study designs included cross-sectional surveys (n = 17), ecological study (n = 1), conceptual paper (n = 1), longitudinal study (n = 1), and prospective cohort study (n = 1). Most were conducted solely in Canada (n = 18), and the remaining included global data. Our content analysis suggested that cannabis consumption during the pandemic varied by reasons for use, consumers’ age, gender, and method of consumption. Health and safety impacts due to the COVID-19 pandemics included increased mental illness, increased emergency visits, and psychosocial impacts.

Discussion
This scoping review suggested that the impact of the pandemic on cannabis consumption in Canada is more complex than simplistic assumptions of an increase or decrease in consumption and continues to be difficult to measure. This study has explored some of those complexities in relation to reasons for use, age, gender, method of consumption, and health impacts. This scoping review is limited by focusing on the breadth compared to depth.

Conclusions
Legalizing nonmedical use of cannabis in Canada in 2018 has had its challenges of implementation, one of which has been the changing context of the society. The findings of this study can help inform cannabis policy updates in Canada as the country is reaching its fifth year of legalizing nonmedical use of cannabis.
A Pediatric Upper Airway Library to Evaluate Interpatient Variability of In Silico Aerosol Deposition
Authors: Kolewe Emily L. , Padhye Saurav , Woodward Ian R. , Feng Yu , Briddell Jenna W. , Fromen Catherine A. 
Published: July 2023

AbstractThe airway of pediatric patients’ changes through development, presenting a challenge in developing pediatric-specific aerosol therapeutics. Our work aims to quantify geometric variations and aerosol deposition patterns during upper airway development in subjects between 3.5 months–6.9 years old using a library of 24 pediatric models and 4 adult models. Computational fluid–particle dynamics was performed with varying particle size (0.1–10 μm) and flow rate (10–120 Lpm), which was rigorously analyzed to compare anatomical metrics (epiglottis angle (θE), glottis to cricoid ring ratio (GC-ratio), and pediatric to adult trachea ratio (H-ratio)), inhaler metrics (particle diameter, $${d}_{p}$$

d
p

, and flow rate, Q), and clinical metrics (age, sex, height, and weight) against aerosol deposition. Multivariate non-linear regression indicated that all metrics were all significantly influential on resultant deposition, with varying influence of individual parameters. Additionally, principal component analysis was employed, indicating that $${d}_{p}$$

d
p

, Q, GC-ratio, θE, and sex accounted for 90% of variability between subject-specific deposition. Notably, age was not statistically significant among pediatric subjects but was influential in comparing adult subjects. Inhaler design metrics were hugely influential, thus supporting the critical need for pediatric-specific inhalable approaches. This work not only improves accuracy in prescribing inhalable therapeutics and informing pediatric aerosol optimization, but also provides a framework for future aerosol studies to continue to strive toward optimized and personalized pediatric medicine.
Graphical Abstract
The Double Democratic Bind: Challenges to Enacting Mandates and Combating Misinformation
Authors: Boräng Frida , Carlitz Ruth 
Published: July 2023

Abstract
Context: Wealthy countries vary considerably in terms of how well they have been able to inoculate their populations against COVID-19. In particular, democracies have been constrained in their abilities to implement vaccine mandates, given enshrined protections of civil liberties and individual freedom in such regimes. While scholars have begun addressing the democratic constraint on vaccine mandates, less attention has been paid to the additional challenges democracies face in constraining the spread of vaccine misinformation—particularly misinformation that spreads online.
Methods: This study combines large-N cross-country analysis with a case study of Germany to illustrate the “double bind” that democracies face when it comes to containing both the spread of disease and the spread of misinformation through social media.
Findings: The cross-national analysis confirms that democracies have been less likely to enact vaccine mandates, and they have also been relatively more hesitant to restrict what people can see and share online. The case study of Germany highlights the normative and the procedural constraints underlying such decisions.
Conclusions: These findings show that resources are often not the binding constraint on effective disease control, raising questions regarding the ability of high-income democracies to respond effectively to future public health emergencies.
Clinical and Imaging Outcomes After Trigeminal Schwannoma Radiosurgery: Results From a Multicenter, International Cohort Study
Authors: Niranjan Ajay , Faramand Andrew , Raju Sudesh S. , Lee Cheng-chia , Yang Huai-che , Nabeel Ahmed M. , Tawadros Sameh R. , El-Shehaby Amr M. N. , Abdelkarim Khaled , Emad Reem M. , Reda Wael A. , Álvarez Roberto Martínez , Moreno Nuria E. Martínez , Liscak Roman , May Jaromir , Mathieu David , Langlois Anne-Marie , Snyder M. Harrison , Shepard Matthew J. , Sheehan Jason , Muhsen Baha'eddin A. , Borghei-Razavi Hamid , Barnett Gene , Kondziolka Douglas , Golfinos John G. , Attuati Luca , Picozzi Piero , McInerney James , Daggubati Lekhaj Chand , Warnick Ronald E. , Feliciano Caleb E. , Carro Eric , McCarthy David , Starke Robert M. , Landy Howard J. , Cifarelli Christopher P. , Vargo John A. , Flickinger John , Lunsford L. Dade 
Published: July 2023

BACKGROUND AND OBJECTIVES:
An international, multicenter, retrospective study was conducted to evaluate the long-term clinical outcomes and tumor control rates after stereotactic radiosurgery (SRS) for trigeminal schwannoma.

METHODS:
Patient data (N = 309) were collected from 14 international radiosurgery centers. The median patient age was 50 years (range 11-87 years). Sixty patients (19%) had prior resections. Abnormal facial sensation was the commonest complaint (49%). The anatomic locations were root (N = 40), ganglion (N = 141), or dumbbell type (N = 128). The median tumor volume was 4 cc (range, 0.2-30.1 cc), and median margin dose was 13 Gy (range, 10-20 Gy). Factors associated with tumor control, symptom improvement, and adverse radiation events were assessed.

RESULTS:
The median and mean time to last follow-up was 49 and 65 months (range 6–242 months). Greater than 5-year follow-up was available for 139 patients (45%), and 50 patients (16%) had longer than 10-year follow-up. The overall tumor control rate was 94.5%. Tumors regressed in 146 patients (47.2%), remained unchanged in 128 patients (41.4%), and stabilized after initial expansion in 20 patients (6.5%). Progression-free survival rates at 3 years, 5 years, and 10 years were 91%, 86%, and 80 %. Smaller tumor volume (less than 8 cc) was associated with significantly better progression-free survival (P = .02). Seventeen patients with sustained growth underwent further intervention at a median of 27 months (3-144 months). Symptom improvement was noted in 140 patients (45%) at a median of 7 months. In multivariate analysis primary, SRS (P = .003) and smaller tumor volume (P = .01) were associated with better symptom improvement. Adverse radiation events were documented in 29 patients (9%).

CONCLUSION:
SRS was associated with long-term freedom (10 year) from additional management in 80% of patients. SRS proved to be a valuable salvage option after resection. When used as a primary management for smaller volume tumors, both clinical improvement and prevention of new deficits were optimized.