bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bayesian‐optimized deep learning for identifying essential genes of mitophagy and fostering therapies to combat drug resistance in human cancers
Authors: Jin Wenyi , Chen Junwen , Li Zhongyi , Yubiao Zhang , Peng Hao 
Published: January 2025

AbstractDysregulated mitophagy is essential for mitochondrial quality control within human cancers. However, identifying hub genes regulating mitophagy and developing mitophagy‐based treatments to combat drug resistance remains challenging. Herein, BayeDEM (Bayesian‐optimized Deep learning for identifying Essential genes of Mitophagy) was proposed for such a task. After Bayesian optimization, BayeDEM demonstrated its excellent performance in identifying critical genes regulating mitophagy of osteosarcoma (area under curve [AUC] of ROC: 98.96%; AUC of PR curve: 100%). CERS1 was identified as the most essential gene regulating mitophagy (mean (|SHAP value|): 4.14). Inhibition of CERS1 sensitized cisplatin‐resistant osteosarcoma cells to cisplatin, restricting their growth, proliferation, invasion, migration and colony formation and inducing apoptosis. Mechanistically, inhibition of CERS1 restricted mitophagy to destroy the mitochondrial quality control in cisplatin‐resistant osteosarcoma cells, including mitochondrial membrane potential loss and unfavourable mitochondrial dynamics, rendering them susceptible to cisplatin‐induced apoptosis. More importantly, mitophagy facilitated the immunosuppressive microenvironment formation by significantly modulating T‐cell differentiation, adhesion and antigen presentation, and mitophagy mainly affects malignant osteoblasts in the early‐mid developmental stage. Immunologically, mitophagy potentially modulated the MIF signalling transmission between malignant osteoblasts and B cells, DCs, CD8+ T cells, NK cells and monocytes through the MIF‐(CD74 + CXCR4) receptor–ligand interaction, thereby modulating the biological functions of these immune cells. Collectively, BayeDEM emerged as a promising tool for oncologists to identify pivotal genes governing mitophagy, thereby enabling mitophagy‐centric therapeutic strategies to counteract drug resistance.
Hypotonic Swelling Method for the Isolation of Pure Mitochondria From Primary Human Skeletal Myoblasts for Proteomic Studies
Authors: Aksu‐Menges Evrim , Kumtepe Eray Taha , Akpinar Gurler , Balci‐Hayta Burcu 
Published: January 2025

ABSTRACTMitochondria play a fundamental role in energy metabolism, particularly in high‐energy‐demand tissues such as skeletal muscle. Understanding the proteomic composition of mitochondria in these cells is crucial for elucidating the mechanisms underlying muscle physiology and pathology. However, effective isolation of mitochondria from primary human skeletal muscle cells has been challenging due to the complex cellular architecture and the propensity for contamination with other organelles. Here, we compared four different methods to isolate mitochondria from primary human skeletal myoblasts regarding total protein yield, mitochondrial enrichment capacity and purity of the isolated fraction. We presented a modified method that combines differential centrifugation with a hypotonic swelling step and a subsequent purification process to minimise cellular contamination. We validated our method by demonstrating its ability to obtain highly pure mitochondrial fractions, as confirmed by Western Blot with mitochondrial, cytosolic and nuclear markers. We demonstrated that proteomic analysis can be performed with isolated mitochondria. Our approach provides a valuable tool for investigating mitochondrial dynamics, biogenesis and function in the context of skeletal muscle biology in health and disease. This methodological advancement opens new avenues for mitochondrial research and its implications in myopathies, sarcopenia, cachexia and metabolic disorders.
Topographic Analysis of the Periorbital Region Including Orbicularis Oculi Muscle Based on Ultrasonography Interpretation
Authors: Lee Kyu‐Lim , Kim Hee‐Jin 
Published: January 2025

ABSTRACTObjectiveUltrasonographic examination is easy, fast, safe, and used in various fields; however, its application to the facial area has been limited. Complex anatomical structures are mixed within thin, soft tissues in the facial region; therefore, understanding their structural characteristics is crucial. This study aimed to use ultrasonography to obtain information on the layered structure and soft tissue thickness of the eye area around the orbicularis oculi muscle and provide guidance for clinical practice.MethodsHealthy volunteers (33 men and 19 women; mean age: 28.4 years) underwent ultrasonography with nine reference points. The soft tissue thickness, including the orbicularis oculi muscle, was measured on monochromatic images. Ultrasonographic scans were performed at facial landmarks using linear transducers (IO8‐17, E‐CUBE15, Alpinion Medical System, Seoul, Korea), with images scanned transversely. The thickness was measured using ImageJ (National Institutes of Health, Bethesda, MD, USA).ResultsThe mean thickness of the orbicularis oculi muscle was 1.56 ± 0.45 mm (range 1.03–2.31 mm). The highest thickness was measured at the points VII (2.31 ± 0.68 mm) and VI (2.15 ± 0.48 mm). The mean depth of the orbicularis oculi muscle was 1.63 ± 0.62 mm (range 0.88–2.80 mm), and the most superficial point was 0.88 ± 0.99, at point VII.ConclusionThis study provides critical anatomical data that can enhance the precision of ultrasound‐guided procedures in the periorbital area, allowing clinicians to accurately target the muscle layer and soft tissue structures. By utilizing these findings, practitioners can optimize treatment effectiveness, reduce complications, and improve outcomes in cosmetic procedures such as botulinum toxin injections, filler placements, and other non‐invasive facial treatments. The detailed anatomical insights gained from this study will help bridge the gap between anatomical understanding and clinical application, promoting safer and more efficient aesthetic interventions.
Birth and pregnancy numbers decreased during the COVID‐19 pandemic in Japan: A time series analysis with the ARIMA model
Authors: Yamamoto Keiko , Uchiyama Koji , Abe Yoshiko , Takaoka Nobuko , Haruyama Yasuo , Kobashi Gen 
Published: January 2025

AbstractAimThe long‐term effects of the COVID‐19 pandemic on birth and pregnancy trends in Japan remain unclear. Although major sporting events are usually followed by an increase in births 9 months later, Japan's fifth wave of COVID‐19 occurred during the Olympics held in Japan during the summer of 2021. In this study, we analyzed how the number of births and pregnancies changed during the COVID‐19 pandemic and large‐scale events in Japan.MethodsWe utilized monthly vital statistical data from birth certificates spanning the years 2010 to 2022. Our analysis followed the identification, estimation, and forecasting stages of autoregressive integrated moving average (ARIMA) modeling. We found the ARIMA (1, 12, 12) model to be adequate for forecasting the monthly number of births.ResultsComparing actual birth data from 2020 to 2022 with our forecast, we observed a significant decrease in births across all of Japan, urban residential areas, and 13 prefectures—primarily metropolitan regions—in January 2021 and May 2022. We also observed a decrease in pregnancy notifications in May 2020, May 2021, and October 2021. The decrease in births in May 2022 in Japan aligns with the decrease in pregnancy notifications 8 months earlier in October 2021.ConclusionsAlthough major sporting events are expected to lead to an increase in the number of births approximately 9 months later, the number of births decreased in May 2022 during the fifth wave of the COVID‐19 pandemic in Japan. These findings suggest that the number of pregnancies and births should be monitored in future pandemics with particular attention to fertility trends.
Are you judging me or my idea? How feedback impacts future idea success in web‐based idea management systems
Authors: Lehmann Selina L. , Beretta Michela , Dao Hung M. , Ebersberger Bernd 
Published: January 2025

AbstractCompanies increasingly rely on web‐based idea management systems (IMS) to source innovative ideas from their employees and external contributors. While existing research emphasizes the critical role of expert feedback in shaping ideators' subsequent contributions, there remains a limited understanding of how various types of feedback—and their interplay—affect future idea success. Grounded in feedback intervention theory, this study examines how success feedback (task‐level), idea‐related failure feedback (task‐level), and ideator‐related failure feedback (self‐level) affect future idea success through shifting ideators' attention and activating different processes. Furthermore, we investigate the moderating roles of feedback readability and timing. Study 1 analyzes 1143 ideas submitted over 5 years by shopfloor employees of an automotive company, while Study 2 examines the causal effects and the underlying processes through an online experiment. The findings reveal that success feedback and constructive idea‐related failure feedback significantly enhance future idea success by activating task motivation and task learning, respectively, while ideator‐related failure feedback shows diminishing returns by activating the meta‐task process. Additionally, lower feedback readability weakens the positive impact of idea‐related failure feedback; however, no significant moderating effect is found for feedback timing. Our research contributes to the IMS literature by demonstrating how different feedback types uniquely influence future idea success. Practically, our findings highlight that experts and managers should prioritize feedback on ideas rather than ideators when providing IMS guidance.
Investigation of Sleep Quality and Intolerance of Uncertainty in Liver Transplant Recipients: A Cross‐Sectional Study
Authors: Savsar Adile , Karayurt Özgül , Kiliç Murat , Kul Gülay Aksu 
Published: January 2025

ABSTRACTLiver transplant recipients experience many uncertainties after transplantation. Also, sleep problems are common among them. This study aimed to examine intolerance of uncertainty and sleep quality in liver transplant recipients. A descriptive and cross‐sectional study was conducted with 117 liver transplant recipients followed in the outpatient clinic of a liver transplant center. Data were collected using a sociodemographic and clinical features form, the Intolerance of Uncertainty Scale‐12 and the Pittsburgh Sleep Quality Index between May and September in 2023. The data were analyzed using descriptive statistics, correlation analysis and regression analysis. The recipients had a high mean score of 41.04 ± 13.11 (min–max: 12–60) on the Intolerance of Uncertainty Scale‐12. Age was predictive of uncertainty. Indeed, young recipients had higher intolerance of uncertainty. The recipients had a low score of 7.33 ± 3.57 (min–max: 1–21) on the Pittsburgh Sleep Quality Index. The prevalence of sleep problems was 65%. Experiencing complications after transplantation was predictive of and worsened sleep quality. The sleep quality of the recipients had no relation with intolerance of uncertainty and inhibitory anxiety, but it had a relation with prospective anxiety.
Nursing and Midwifery Students' COVID‐19 Vaccine Regrets and Future Vaccination Intentions: A Mixed Methods Study
Authors: Tayhan Ali , Bozhan Tayhan Esra , Şahin Büyük Damla 
Published: January 2025

ABSTRACTRegret over COVID‐19 vaccine decisions is a post‐pandemic phenomenon that needs further research. This mixed‐method research was conducted to examine nursing‐midwifery students' COVID‐19 vaccine decision regret and their intention to get vaccinated in the next pandemic. The research includes quantitative and qualitative research processes. In the first phase, a quantitative (cross‐sectional) study was conducted, collecting data from 602 participants who met the inclusion criteria. In the second phase, semi‐structured interviews were conducted with 16 participants. Data were collected using the Decision Regret Scale and semi‐structured interview form. Data were analyzed using Student's t‐test, one‐way anova test, and inductive content analysis, and reported using Good Reporting of a Mixed Methods Study. In the study, it was determined that 96.3% of the participants had received the COVID‐19 vaccine and 50.0% regretted getting vaccinated. As a result of the analysis of qualitative data, the following themes emerged that could reveal the reasons for vaccine regret; forced compliance, a strange pandemic, unknown effects, and vaccine hesitancy. Students' experiences in the COVID‐19 pandemic have the potential to increase negative attitudes towards their vaccines.
Effect of Thickened Fluids on Swallowing Function in Oropharyngeal Dysphagia: Impact of Shear Rheology and Disorder Subtype
Authors: Omari T. , Ross A. , Schar M. , Campbell J. , Thompson A. , Besanko L. , Lewis D. A. , Robinson I. , Farahani M. , Cock C. , Mossel B. 
Published: January 2025

ABSTRACTIntroductionFluid thickeners used in the management of oropharyngeal dysphagia exhibit non‐Newtonian shear‐thinning rheology, impacting their viscosity during deglutition. This study investigated how the rheological properties of thickened fluids affect pharyngeal swallowing parameters in patients with oropharyngeal motor disorders diagnosed by pharyngeal high‐resolution manometry impedance (P‐HRM‐I).MethodsSeventy‐two patients (18–89 years) referred for P‐HRM‐I were diagnostically assessed with a 10 mL thin bolus. In 57 of the patients, 10 mL swallows of two moderately thick formulations—xanthan gum (XG) and sodium carboxymethylcellulose gum (CMC)—were also tested. The XG and CMC fluids had equivalent empirical thickness but different viscosity at pharyngeal phase shear rates: XG 87 mPa.s (83–91) versus CMC mean 157 mPa.s (148–164) at 300 s−1. Standard metrics of pharyngeal and upper esophageal sphincter (UES) function were derived from P‐HRM‐I recordings and analyzed to characterize patients into one of four disorder subtypes: (i) No Disorder, (ii) UES Disorder, (iii) Pharyngeal Disorder, and (iv) Combination UES/Pharyngeal Disorder. Impedance recordings also assessed pharyngeal bolus transit.ResultsPatients with a Combination UES/Pharyngeal Disorder were most likely to have abnormal bolus transit (82%, p < 0.001). Increasing bolus viscosity significantly influenced UES residual pressure, UES opening area, and post‐swallow residue. Patients with UES Disorder exhibited pronounced increases in UES residual pressure with CMC compared to XG. Pharyngeal contractility was unaffected by viscosity changes. Post‐swallow residue increased with CMC, particularly in patients with a Combination Disorder. Case‐by‐case analysis revealed individual variability in response to the different viscosities.ConclusionThe rheological properties of thickened fluids significantly affect swallowing function, with these effects dependent upon the disorder subtype.
Disrupted Paraventricular Hypothalamic Nucleus Functional Connectivity in Parkinson's Disease With Constipation
Authors: Hou Xiaorong , Zhou Hongfei , Zhou Qiugui , Zhang Jiajian , Tang Xuxiong , Gong Ziwei , Tang Ying , Duan Junhong , Peng Song , Li Lifeng , Jiang Hong , Tang Beisha , Liu Yin , Lei Lifang 
Published: January 2025

ABSTRACTBackgroundConstipation is one of the most common non‐motor symptoms in patients with Parkinson's disease (PD), which could manifest during the early stage of the disease. However, the etiology of constipation in PD remains largely unknown. Previous studies supported that gastrointestinal dysfunction may be associated with functional connectivity alterations in paraventricular hypothalamic nucleus (PVN). Therefore, this study aimed to investigate the potential contribution of the PVN to the pathogenesis of constipation in a cohort of early‐stage patients with PD and to compare brain network organization between PD patients with and without constipation.MethodsA total of 66 PD patients (PD with constipation and without constipation) and 30 healthy controls were prospectively enrolled. All participants acquired T1‐weighted and resting‐state fMRI scans. Then we employed voxel‐based morphometry analysis and functional connectivity analysis.ResultsWe observed a decreased functional connectivity in the PVN‐pontine tegmentum pathway in PD patients with constipation compared to the patients without constipation (p = 0.006, t = 5.37), while we did not find any changes in basal ganglia circuitry between these two groups. In addition, we found that the functional connectivity between PVN and pontine tegmentum was negatively associated with the UPDRS I, II, III and NMSS scores (p < 0.05). Meanwhile, these two types of patients also showed substantial differences in functional connections linking the inferior frontal gyrus and cerebellum with multiple brain regions. We discovered no statistical difference in gray matter volume among these two groups.ConclusionsOur study provides further insights into the dysfunctional mechanisms of constipation, suggesting that abnormal PVN functional connectivity may be related to the mechanism of constipation in PD. Meanwhile, the inferior frontal gyrus and cerebellum may be involved in the occurrence of constipation in PD patients.
Loops and the geometry of chance
Authors: Jäger Jens 
Published: January 2025

AbstractSuppose your evil sibling travels back in time, intending to lethally poison your grandfather during his infancy. Determined to save grandpa, you grab two antidotes and follow your sibling through the wormhole. Under normal circumstances, each antidote has a 50% chance of curing a poisoning. Upon finding young grandpa, poisoned, you administer the first antidote. Alas, it has no effect. The second antidote is your last hope. You administer it—and success: the paleness vanishes from grandpa's face, he is healed. As you administered the first antidote, what was the chance that it would be effective? This essay offers a systematic account of this case, and others like it. The central question is this: Given a certain time travel structure, what are the chances? In particular, I'll develop a theory about the connection between these chances and the chances in ordinary, time‐travel‐free contexts. Central to the account is a Markov condition involving the boundaries of spacetime regions.
Apoplastic pH is a chemical switch for extracellular H2O2 signaling in abscisic acid‐mediated inhibition of cotyledon greening
Authors: Zhou Miao , Ye Jia Yuan , Shi Yi Ju , Jiang Yi Jie , Zhuang Yao , Zhu Qing Yang , Liu Xing Xing , Ding Zhong Jie , Zheng Shao Jian , Jin Chong Wei 
Published: January 2025

Summary
The apoplastic pH (pHApo) in plants is susceptible to environmental stimuli. However, the biological implications of pHApo variation have remained largely unknown.
The universal stress phytohormone abscisic acid (ABA) as well as the major environmental stimuli drought and salinity were selected as representative cases to investigate how changes in pHApo relate to plant behaviors in Arabidopsis. Variations in pHApo negatively regulated the cotyledon greening inhibition to the universal stress hormone ABA or environmental stimuli through the action of extracellular hydrogen peroxide (eH2O2).
Further studies revealed that an increase in pHApo diminishes the chemical reactivity of eH2O2, effectively functioning as an ‘off’ switch for its action in oxidizing thiols of plasma membrane proteins. Consequently, this suppresses the eH2O2‐mediated cotyledon greening inhibition to environmental stimuli and ABA, alongside inhibiting the eH2O2‐mediated intracellular Ca2+ signaling. Conversely, a decrease in pHApo serves as an ‘on’ switch for the action of eH2O2.
In summary, the pHApo is a crucial messenger and chemical switch for eH2O2 in signal transduction, notwithstanding the apparent simplicity of the underlying mechanism. Our findings provide a novel fundamental biological insight into the significance of pH.
Love first
Authors: White P. Quinn 
Published: January 2025

AbstractHow should we respond to the humanity of others? Should we care for others' well‐being? Respect them as autonomous agents? Largely neglected is an answer we can find in the religious traditions of Judaism, Christianity and Buddhism: we should love all. This paper argues that an ideal of love for all can be understood apart from its more typical religious contexts and moreover provides a unified and illuminating account of the the nature and grounds of morality. I defend a novel account of love for all that avoids serious worries about the incoherence or impossibility of loving everyone. Doing so requires countenancing a neglected form of love. Love admits as its object not just individual entities like people and groups; we can also bear a love for the Fs in general—for all the Joneses, all the philosophers, or even all the human beings. I go on to argue that while it is possible for ordinary agents like us to love all, we shouldn't. Instead, we should approximate love for all. The minimal approximation of love for all is, surprisingly, respect; I derive the basic, structural features of deontological ethics (including anti‐paternalism and anti‐aggregation) from the ideal of loving all.
Relationship between apathy/post‐stroke depression and gait training in patients with stroke
Authors: Maeda Takashi , Shishido Kenichiro , Ouchida Tomoki , Moriuchi Yasuyuki , Hayashi Atsuko 
Published: January 2025

AbstractBackgroundWe examined the effect of gait training on apathy/post‐stroke depression (PSD) in patients with walking disorders after stroke, and the effect of apathy/PSD on gait reacquisition in subacute stroke.MethodsFifty‐five participants with gait disorders after stroke underwent gait training for 6 weeks. Outcome measurements included Apathy Scale (AS), Center for Epidemiologic Studies Depression Scale (CES‐D), Mini‐Mental State Examination, Lower Extremity Function of Stroke Impairment Assessment Set, and Functional Independence Measure‐TRANSFER and ‐WALK scores. These scores of the participants were divided into groups with or without apathy based on AS scores for statistical analysis.ResultsBoth groups showed significant differences in all outcomes after gait training. In addition, even if the participants had apathy at baseline, there were no significant differences in outcomes other than AS/CES‐D between the two groups after gait training. Logistic regression analysis showed that only Mini‐Mental State Examination and Lower Extremity Function of Stroke Impairment Assessment Set scores affected the reacquisition of independent walking (odds ratio: 1.332, P = 0.015; odds ratio: 1.364, P = 0.005, respectively). AS and CES‐D scores had no effects on gait reacquisition.ConclusionGait training may be beneficial for both physical impairment and psychiatric symptoms in patients with stroke and may prevent poor functional recovery caused by apathy and PSD. As opposed to previous studies that have shown a negative impact of apathy and PSD on functional recovery, our results suggest that apathy/PSD may not negatively affect functional recovery when gait training is conducted in subacute stroke.