bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of 5-o-(n-boc-l-alanine)-renieramycin t in the suppression of lung cancer stem cells
Authors:   , Suksamai Darinthip 
Published: September 2023

Cancer stem cells (CSCs) drive aggressiveness and metastasis by utilizing stem cell-related signals. In this study, 5-O-(N-Boc-L-alanine)-renieramycin T (OBA-RT) was demonstrated to suppress CSC signals and induce apoptosis. OBA-RT exerted cytotoxic effects with a half-maximal inhibitory concentration of approximately 7 µM and mediated apoptosis as detected by annexin V/PI and nuclear staining assays. Mechanistically, OBA-RT exerted dual roles, activating p53-dependent apoptosis and concomitantly suppressing CSC signals. A p53-dependent pathway was indicated by the induction of p53 and the depletion of anti-apoptotic Mcl-1 and Bcl-2 proteins. Cleaved PARP was detected in OBA-RT-treated cells. Interestingly, OBA-RT exerted strong CSC-suppressing activity, reducing the ability to form tumor spheroids. In addition, OBA-RT could induce apoptosis in CSC-rich populations and tumor spheroid collapse. CSC markers, including CD133, Oct4, and Nanog, were notably decreased after OBA-RT treatment. Upstream CSCs regulating active Akt and c-Myc were significantly decreased; indicating that Akt may be a potential target of action. Computational molecular modeling revealed that OBA-RT could interact with an Akt molecule with high affinity. This study has revealed a novel CSC inhibitory effect of OBA-RT via Akt inhibition, which may improve cancer therapy.
Effect of hydroquinone 5-o-cinnamoyl ester of renieramycin m on the suppression of lung cancer stem cells
Authors:   , Hongwiangchan Nattamon 
Published: September 2023

Cancer stem cells (CSCs) are distinct cancer populations with tumorigenic and self-renewal abilities. CSCs are drivers of cancer initiation, progression, therapeutic failure, and disease recurrence. Thereby, novel compounds targeting CSCs offer a promising way to control cancer. In this study, the hydroquinone 5-O-cinnamoyl ester of renieramycin M (CIN-RM) was demonstrated to suppress lung cancer CSCs. CIN-RM was toxic to lung cancer cells with a half-maximal inhibitory concentration around 15 µM. CIN-RM suppressed CSCs by inhibiting colony and tumor spheroid formation. In addition, the CSC population was isolated and treated and the CSCs were dispatched in response to CIN-RM within 24 h. CIN-RM was shown to abolish cellular c-Myc, a central survival and stem cell regulatory protein, with the depletion of CSC markers and stem cell transcription factors ALDH1A1, Oct4, Nanog, and Sox2. For up-stream regulation, we found that CIN-RM significantly inhibited Akt and consequently decreased the pluripotent transcription factors. CIN-RM also inhibited mTOR, while slightly decreasing p-GSK3β (Ser9). Inhibiting Akt/mTOR induced ubiquitination of c-Myc and promoted degradation. The mechanism of how Akt regulates the stability of c-Myc was validated with the Akt inhibitor wortmannin. Taken together, we utilized molecular experiments and the CSC phenotype to reveal the novel suppressing the activity of this compound on CSCs to benefit CSC-targeted therapy for lung cancer treatment.
The effect of population structure on economic development: Thailand's provincial panel data
Authors:   , Pattarakijkusol Satayu 
Published: September 2023

This research investigates the effects of demographic change, the change of labor supply, and human capital on Thailand’s economic development. This will help shed light on the relationship between population (quantitatively and qualitatively) and economic development. The demographic dividends, the neoclassical growth Solow-Swan model, and human capital are adopted as the conceptual framework for this research. The secondary panel data at the provincial level from government officials will be used for statistical analysis. Fixed effect or random effect with lagged dependent variable is introduced into the estimated model. The finding suggests that the labor productivity, employment level, share of the active labor force have significant and strongly positive to Thailand’s economic development. While youth dependency ratio has a significant and strong negative effect on Thailand’s economic development. The rest of the variables have weakly effect on Thailand’s economic development. In addition, there are some findings that reversed the original proposal which is the old-age dependency ratio that has a significant and weakly positive effect on Thailand’s economic development and the share of the working-age population that has a significant and weakly negative effect on Thailand’s economic development. The finding suggests that Thailand should benefit from labor productivity and enjoy benefits from this. In the long run, the government to develop labor productivity, this may be achieved through training, lifelong learning, and education among other means. Concerning the negative of the youth dependency ratio Thailand’s economic development, the research findings suggested that the reduction of the youth dependency ratio would lead to better economic development. However, the decrease in the youth dependency ratio also implies a lower number of labor force who will enter the labor market in the future. This will also negatively impact Thailand’s economic development. Therefore the problem will become loops. the government must pay more attention to the fertility policy because a good fertility policy will increase the birth rate, which will impact the country’s future labor supply. This policy must strike a balance between the short- and the long-run. If there are too many births, this will increase dependency. On the contrary, without enough births, there would be a shortage of labor.
Fear appeals for increasing intentions to have colorectal cancer screening among high-risk adults: moderating role of tripartite self-construal
Authors:   , Sa-ngasri Suprapa 
Published: September 2023

Colorectal cancer (CRC) is considered a threat to life. Early detection is key to effective treatment. Fear appeal is one of the most famous techniques to persuade people to uptake CRC screening. This current study attempted to replicate the congruent effect of cultural orientation at an individual level and fear message types. A 3 (self-construal) x 2 (self-threatened versus family-threatened) factorial research design was employed to examine the effect of tripartite self-construal and the types of fear message on the perceived threat, feelings of fear, attitude and intention toward CRC screening among Thais. One hundred and thirty-three adults aged between 40 and 70 who lived in Bangkok were then randomly assigned to one of six experimental conditions via online participation. The between-group Multivariate Analysis Of Covariance (MANCOVA) indicated no difference among experimental conditions. However, the results were not conclusive due to failed manipulation checks on both self-construal and fear message type manipulations. The result from PROCESS analysis on measured self-construal revealed some significant relationships among variables, especially the relationship between perceived CRC threat and fear appeal outcomes. Despite the non-significant results, practitioners and future researchers would gain insights about implementing fear appeal messages for attitude and behavioral change to promote CRC screening.
Assessment of knowledge, attitude and usage on personal protective equipment among mechanical maintenance workers of a power generation unit in Thailand
Authors:   , Tankian Titiphorn 
Published: September 2023

A Power Generation Unit has duty of electric power supply to the people by producing and distributing electric power. The mechanical maintenance department is one part of their responsibility to achieve the target. However, 207 accident cases were reported during 2012 to 2019. The main causes were found to be unsafe act and unsafe condition including improper usage and lack of personal protective equipment (PPEs). Thus, knowledge, attitude regarding to PPEs, its usage, and factors related to PPEs usage of mechanical maintenance workers need to be assessed. This survey study collected data through self-response questionnaire from 379 mechanical maintenance workers of a power generation unit in Thailand. The average age of the workers is 42 years. Most of workers are man and 80.7% have a position as technician. Most of them have ever passed safety training before starting working which wearing PPEs was a part of safety training. Half of workers got injury from accident while working. The results reveal that 43.3%, 54.6%, and 2.1% of the workers possess level of knowledge at high, moderate, and low respectively. Only 20.8% showed high level of attitude on PPE usage. Moreover, 77% of them did not regularly use recommended PPEs while working, among these, 60% of them did not use regular PPEs and a few used particular PPEs. The association between attitude and practice was significantly low positive correlation. Besides, attitude also associated with knowledge. No association between knowledge and practice was found. Related factors can affect to usage PPEs (p-value<0.05); i.e., job position, department, education, times of safety training), injury’s history, and attitude. The results of the study suggested that policy and program is necessary enhance the awareness of the benefit of wearing PPEs and training should be introduced for workers in Thailand. The further study should be conducted to investigate the reason behind cause of non-use PPEs during working.
Effects of extensive reading on reading fluency and reading comprehension of primary grade students in Bhutanese ESL context
Authors:   , Pem Kinley 
Published: September 2023

This study explored the effects of extensive reading on reading comprehension and reading fluency of Bhutanese primary grade students. The participants were 16 fourth grade students in one of the primary schools in Bhutan. The study involved 12 weeks of extensive reading instruction. Participants were introduced to extensive reading, acquainted to selecting appropriate books, and a large number of graded readers with enjoyable content were supplied, and participants read as many books as they could. For the quantitative data, pre-and post-test results of reading comprehension and reading fluency were compared using the Wilcoxon signed-rank test. For qualitative data, semi-structured interviews and reading log responses were analyzed to determine the impacts of extensive reading instruction and to explore students' opinions toward it. The findings showed that the English reading fluency pre- and post-test mean scores differed significantly (p<.05). There was also a significant difference (p<.05) in the English reading comprehension pre- and post-test mean scores. Similarly, the qualitative findings from reading logs also depicted drastic improvement in reading fluency and reading comprehension scores in the final week. The semi-structured interview responses revealed that almost all the students preferred choosing books on their own. Students preferred books that are appropriate with pleasurable content. Some students liked reading books at home while some of them favored school. The responses also showed students improvement in reading fluency, reading comprehension, reading motivation, vocabulary, and writing. The findings suggested extensive reading instruction be implemented in other Bhutanese second language learning classrooms due to its massive gains in developing language proficiencies of young learners.
Enhanced heterogeneous network model with ensemble similarities for identifying protein targets of drugs
Authors:   , Tangmanussukum Piyanut 
Published: September 2023

Currently, computational identification of drug target proteins is widely used to help saving cost and time for drug discovery and development. One of the most efficient approaches is a prediction of drug-target interactions based on similarity scores between drugs and target proteins. Despite various data about drugs and targets extensively available, only chemical structures and protein sequences are mostly used to compute drug-drug and target-target similarity scores, respectively. In this thesis, the Forward similarity integration (FSI) Framework is proposed for systematically integrating multiple similarity measures to construct a heterogeneous network propagation model with a suitable similarity integration. Seven drug-similarity measures, nine target-similarity measures, and four similarity integration methods were formulated and used in the FSI framework. Thus, the suitable heterogeneous network model combines three drug-similarity measures integrated by using similarity network fusion (SNF) and one target-similarity measure based on protein sequences. The model selected by FSI reached an accuracy of 99.8% and significantly outperformed the models with random, full similarity integration, and the models without similarity integration. Also, the case studies of newly discovered drug-target interactions demonstrate the practicality of the proposed method for drug-target interaction prediction.
Efficacies of Thai probiotic strains against antibiotic resistance bacteria and modulate gut microbiome and resistome in pig model
Authors:   , Apiwatsiri Prasert 
Published: September 2023

Lactic acid bacteria (LAB) have been widely used as probiotics in the livestock industry because of their high potential for antibacterial activity, anticonjugation, antibiofilm capacity, and gut microbiome regulation. Lactobacillus plantarum 22F, 25F (L22F and L25F), and Pediococcus acidilactici 72N (P72N) showed several promising in vitro and in vivo properties, according to our recent research. The objective of this study was to evaluate the Thai LAB efficacy on anticonjugation and antibiofilm activities in E. coli harboring the mcr-1 gene. When compared to the neutralizing cell-free supernatant (CFS), we discovered that the CFS derived from our LAB strains at 1:16 dilution (pH 5.70-5.92) significantly reduced the transfer frequency of the mcr-1 gene between the donor and recipient E. coli by up to 100 times (pH 6.5). Furthermore, our non-neutralizing CFS has the potential to significantly reduce the production of E. coli biofilm by more than 82 % for planktonic biofilm and 60 % for sessile biofilm, respectively. It has the potential to inhibit biofilm development in the planktonic stage by up to 52 % when used to neutralize CFS. The effect of our LAB on the gut microbiota and resistome in weaned piglets with and without enterotoxigenic Escherichia coli (ETEC) infection was monitored and observed using whole-genome shotgun metagenomics. The findings revealed that a multi-strain LAB containing L22F, L25F, and P72N could help to expand beneficial bacteria families such as Lactobacillaceae, Lachnospiraceae, and Ruminococcaceae. In comparison to antibiotic pigs, it also lowered beta-lactam resistance, copper resistance, multi-biocide resistance, tetW, and tetQ. In piglets treated with our LAB strains, IncX4, a plasmid associated with colistin resistance, was reduced, and an integrase gene (intI) class 2 and 3 could not be detected. However, this group was also enriched in the insertion sequences (IS) 3 and 30, which are linked to nutrition use. Furthermore, in LAB supplemented piglets, the detoxification and oxidative stress response, which is connected to antioxidant activity, as well as amino acid and glucose metabolism, were all boosted. Our findings may highlight the LAB properties inhibiting antimicrobial resistance bacteria, positively affecting gut microbiome, and resistome modulation based on metagenomic research.
A serological and molecular survey of SARS-CoV-2 infection in domestic dogs and cats in Bangkok and vicinity Thailand
Authors:   , Udom Kitikhun 
Published: September 2023

The COVID-19 pandemic caused by SARS-CoV-2 has significant impact to human health worldwide. As of June 2022, over 500 million human cases with 6 million deaths have been reported. Despite SARS-CoV-2 infection in human, SARS-CoV-2 infections in domestic animals have also been reported due to spillover of the viruses from human-animals interface. The aim of this study was to conduct serological and molecular detection of SARS-CoV-2 in dogs and cats in Bangkok and vicinity in Thailand during the pandemic outbreaks. A large-scale SARS-CoV-2 survey in domestic animals which a total of 4,696 serum samples (2,960 of dogs, 1,736 of cats) and 363 swabs (n=174 of 87 dogs, n=189 of 92 cats) were collected from April 2020 to August 2021. Serum samples were tested for SARS-CoV-2 antibodies using multi-species ELISA and surrogate virus neutralization test (sVNT). Swab samples were tested for SARS-CoV-2 RNA by real-time RT-PCR with four panels of specific primers and probes. The ELISA results showed that 1.79% (53/2,960) of dogs and 0.35% (6/1,736) of cats were seropositive. Most animals with seropositivity showed no clinical signs. Positive and suspected serum samples were subjected to sVNT test and the results showed that neutralizing antibodies was detected in a cat (n=1) suggesting SARS-CoV-2 infection in animals during the pandemic in Thailand. However, all swab samples were negative for SARS-CoV-2 RNA. This thesis showed the benefit of disease surveillance in domestic animals during the pandemic. Thus, routine surveillance and monitoring of SARS-CoV-2 in animals are necessary for planning the prevention and control strategies for COVID-19 in human and animals.
Identification, characterization and pathogenicity of aeromonas schubertii isolated from diseased striped snakehead fish (Channa Striata)
Authors:   , Budiyansah Hendri 
Published: September 2023

Striped snakehead fish (Channa striata) is one of the popular cultivated fish in Thailand. However, it is inevitable to be susceptible to abundant pathogens, especially Aeromonas schubertii. In July 2020, an unknown outbreak was occurred on striped snakehead fish nursery farm in Nakhon Pathom province, Thailand. Suspected six isolates were A. schubertii yielded from the fish as the main causative agent. This research aimed to characterize and examine pathogenicity of A. schubertii isolated from diseased striped snakehead fish. Characterization was performed based on combination of biochemical assay, PCR and DNA sequencing (based on 16S rRNA and gyrB), and MALDI-TOF MS. The results showed the A. schubertii isolates can be distinguished from other bacterial pathogens by its biochemical characteristics, DNA fingerprints (16S rRNA and gyrB), and protein mass fingerprints. The experimental challenge of A. schubertii, using intra-peritoneal (IP) injection on C. striata, showed 80% mortality on high dose (1.2x108) and median lethal dose (LD50) of 3.9x105. A significant manifestation of skin ulceration, internal organ hemorrhage and white nodules on the fish internal organs (liver, kid-ney, and spleen) was progressively seen on 7-, 14-, and 21-days of post injection (dpi). The white nodules significantly appeared at the liver, spleen, and kidney, respectively. Likewise, granulomas, melano-macrophage centers (MMCs) and necrotic cells were also observed in histopathology analysis. Moreover, it was discovered that white nodules on kidney and spleen were strongly correlated with granulomas observed on kidney and spleen histopathology.
Degree of genetic admixture in hybrid populations between macaca fascicularis fascicularis and M. F. aurea in association with stone tool-use behavior
Authors:   , Phadphon Poompat 
Published: September 2023

Burmese long-tailed macaque (Macaca fascicularis aurea) is the only Old World monkey that habitually uses stones as tools to access encased food. The natural range of M. f. aurea closely contacts to the common long-tailed macaque (M. f. fascicularis) in southern Thailand at 8°10'-12°24'N, where the intraspecific hybridization between the two subspecies was reported. While M. f. aurea x M. f. fascicularis hybrids were expressed a stone-tool use behavior, the behavior has never been seen in the wild or captive M. f. fascicularis. It is interesting to understand if the genetic factor plays a role on the emergence of this behavior. This study surveyed the populations of M. f. aurea, M. f. fascicularis and hybrids at the intraspecific hybrid zone and vicinity, identified morphological subspecies, observed/tested the stone-tool use behaviors, collected blood and fecal samples for genetic analyses, and genetic subspecies identification using mtDNA, Y-chromsome genes (SRY and TSPY), and autosomal single nucleotide polymorphism (SNP) derived from Restriction Site Associated DNA Sequencing (RADseq) as genetic markers. The findings in this study illustrated the influence of the early-middle Pleistocene transition (EMPT) on the split of M. f. aurea lineage through the southward migration from Myanmar/Bangladesh to southwestern Thailand. This study confirmed the previous hypothesis of the male-mediated intraspecific hybridization from M. f. aurea to M. f. fascicularis populations. In addition, the analysis of RADseq-derived SNPs indicated an asymmetrically (or unidirectionally) genetic introgression of M. f. aurea to M. f. fascicularis populations that occurred far beyond the previously reported intraspecific hybrid zone. The analysis of an association between the identified morphological subspecies or identified genetic subspecies and the stone-assisted behaviors, that were categorized into 4 levels; stone-tool use behavior, food pounding behavior, stone-play behavior and none, was performed. The statistically significant association between the morphologically identified M. f. aurea and hybrids and the complex stone-associated behaviors was detected, while it was no association between the identified genetic subspecies (based on mtDNA, SRY and TSPY) and the stone-associated behaviors. However, the SNP markers that indicate the degree of genetic admixture in hybrid populations can associate the high degree of M. f. aurea ancestry in M. fascicularis with stone-play and stone-tool use behaviors and the low degree of M. f. aurea ancestry in M. fascicularis with non-stone use behavior. Thus, these results imply the possibility of genetic influences on the emergence, the prevalence and the restriction of stone-tool use behavior in M. f. aurea and hybrids.
ผลของชาร์จากไม้ไผ่และกะลาปาล์มที่ถูกกระตุ้นด้วยไอน้ำต่อการผลิตน้ำมันดิบชีวภาพจากชานอ้อยโดยไฮโดรเทอร์มัลลิควิแฟกชัน
Authors:   , นิลอ่อน ณัฐชนน 
Published: September 2023

ปัจจุบันแหล่งพลังงานหลักคือก๊าซธรรมชาติและน้ำมันดิบจากกระบวนการขุดเจาะน้ำมันของโลก การใช้วัสดุข้างต้นทำให้เกิดก๊าซคาร์บอนไดออกไซด์ (CO2) เป็นจำนวนมาก และเป็นปัจจัยหลักที่ทำให้เกิดภาวะโลกร้อน การลดการปล่อย CO2 ทำได้โดยการใช้ชีวมวลเป็นแหล่งพลังงานแทนการขุดเจาะน้ำมัน กระบวนการไฮโดรเทอร์มัลลิควิแฟกชันเป็นกระบวนการที่น่าสนใจที่สามารถแปลงชีวมวลเป็นผลิตภัณฑ์เหลวหรือผลิตภัณฑ์ชีวภาพ การใช้ตัวเร่งปฏิกิริยาร่วมในกระบวนการช่วยปรับปรุงคุณภาพและผลผลิตของผลิตภัณฑ์ของเหลวจากกระบวนการไฮโดรเทอร์มัลลิควิแฟกชัน ชาร์ถือเป็นตัวเร่งปฏิกิริยาในกระบวนการที่มีราคาถูกและสามารถผลิตได้ง่ายจากชีวมวล นอกจากนี้การกระตุ้นชาร์ด้วยไอน้ำยังช่วยเพิ่มสมบัติเป็นตัวเร่งปฏิกิริยาได้ดีขึ้น ในงานวิจัยนี้ ได้ทำการศึกษาผลของชาร์ที่ถูกกระตุ้นด้วยไอน้ำต่อผลผลิตและคุณภาพของน้ำมันดิบชีวภาพ โดยการทำไฮโดรเทอร์มัลลิควิแฟกชันดำเนินการในเครื่องปฏิกรณ์ความดันสูงแบบแบตช์ที่อุณหภูมิ 300-350 องศาเซลเซียส ที่ความดันเริ่มต้น 2 เมกะพาสคัล โดยใช้เวลาในการทำปฏิกิริยา 60 นาที ชาร์ถูกกระตุ้นโดยไอน้ำที่อุณหภูมิ 800-900 องศาเซลเซียส โดยใช้เวลาในการกระตุ้น 120 นาที จากผลการทดลองพบว่า ร้อยละผลได้ของน้ำมันดิบเพิ่มขึ้นเมื่อใช้ชาร์กะลาปาล์มและชาร์ไม้ไผ่ร่วมด้วย เนื่องจากชาร์มีผลในการเร่งปฏิกิริยาการแตกตัวของชีวมวล ในส่วนของอุณหภูมิในการทำปฏิกิริยาที่ 300 และ 350 องศาเซลเซียส พบว่าเมื่อใช้อุณหภูมิในการทำปฏิกิริยาที่สูงขึ้น ร้อยละผลได้ของน้ำมันดิบจะลดลงในขณะที่ผลิตภัณฑ์ที่เป็นแก๊สเพิ่มขึ้น นอกจากนี้ เมื่อใช้ชาร์ที่ถูกกระตุ้นด้วยไอน้ำร่วมในกระบวนการกระบวนการไฮโดรเทอร์มัลลิควิแฟกชัน จะส่งผลช่วยปรับปรุงคุณภาพของน้ำมันชีวภาพโดยพิจารณาจากค่าความร้อนสูง