bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does hysterectomy for benign indications decrease the risk of ovarian cancer? A national population-based study
Authors: Yuk Jin-Sung , Lee Jung Hun 
Published: August 2023

Abstract
This study aimed to assess the impact of hysterectomy for benign gynecologic conditions on ovarian cancer incidence and all-cause mortality among women diagnosed with ovarian cancer. Using Korean health insurance data (2007–2020), a retrospective cohort study focused on women aged 40 to 59 years undergoing hysterectomy (with or without adnexal surgery) between 2011 and 2014. The control group included same-aged women receiving health exams during the same period. After 1:1 propensity matching, the incidence of ovarian cancer and all-cause mortality were compared. Out of 133,288 women undergoing medical exams and 70,787 undergoing hysterectomy, 70,787 controls were matched. Median ages for control and hysterectomy groups were 46 and 47 years, respectively. Ovarian cancer incidence was 0.1% (92/70,695) in controls and 0.2% (111/70,676) in the hysterectomy group (P = 0.182). Stratified multivariate analysis found no significant difference in ovarian cancer risk between groups (adjusted hazard ratio 1.213, 95% CI 0.902–1.63). In ovarian cancer patient subgroups, all-cause mortality didn't significantly differ between hysterectomy and control groups (adjusted HR 0.932, 95% CI 0.363–2.393). In conclusion, hysterectomy with or without adnexal surgery didn't impact ovarian cancer incidence or overall survival.
Non-alcoholic steatohepatitis in pregnancy: case report
Authors: Cordivani Jonathan , Clotilde Lamy , Michel Boulvain , Catherine Riera 
Published: August 2023

Abstract
Background: Dyslipidemia and non-alcoholic fatty liver disease (NAFLD) are well known diseases and are part of a very broad spectrum evolving towards non-alcoholic steatohepatitis (NASH). This entity has been very rarely described by pregnant women.
Case presentation: A 26 years old woman with 28+5weeks of pregnancy presented with preterm labor. At admission, a significant hepatic cytolysis was detected (AST/ALT 265/485 U/L - GGT/alkaline phosphatase 60/164 U/L). She had normal blood pressure and negative 24-hour proteinuria. She didn’t have intrahepatic cholestasis of pregnancy (bile acid at 7) and also no organic hepatic etiology (negative serologies and imaging). This cytolysis worsened until a more detailed history revealed a potential etiology: a diet very rich in sugars and fatty acids corresponding to a NASH syndrome. Dietary rebalancing improved the cytolysis. Complications such as intrahepatic cholestasis of pregnancy and preterm birth favored by significant dyslipidemia could not be avoided.
Conclusion: it is important to consider the lipid profile of our patients. This will allow for a more personalized follow-up given the multiple obstetrical repercussions that can arise from this pathology. It should also be considered in the differential diagnosis of liver test alterations during pregnancy. A healthy diet seems to help control the disease
Residual efficacy of Clothianidin (SumiShield 50WG) used in experimental huts conditions and then in Indoor Residual Spraying campaign in Senegal
Authors: Ciss Oumar , Sy Ousmane , DIOUF El Hadji , Fall Moussa , Ndiaye Assane , Gueye Marème , Diop Moussa , Ndiouck Babacar , Diagne Moussa , Faye Ousmane , Niang El Hadji Amadou , Konate Lassana 
Published: August 2023

Abstract
Background
In Senegal, the main vector control strategies include implementing indoor residual spraying (IRS) and distribution of long-lasting insecticides insecticidal nets (LLINs). Drugs resistance and insecticides resistance use on public health were appearing affected to combatted malaria transmission. In the country vectors became resistant to pyrethroids, the insecticides class that nets are impregnate. As they affected strategies for vector control, new formulation belonging on neonicotinoid class, clothianidin was developed and used for malaria vector control through IRS of insecticide.
Methods
Residual efficacy of clothianidin treated walls was assessed monthly using cone bioassays following WHO method. Five houses in each of the two villages sprayed were assessed and one unsprayed house served as the control. In trial huts experimental, six huts in total, including three in banco and three others in cement treated were assessed monthly. Three cones were installed on three walls in each of the sprayed houses at 0.5 m, 1m and 1.5m above the floor, and three cones at the control house. About 10 females, 2–5 -days old from the laboratory susceptible strain of An. coluzzii were exposed in each cone for 30 mins and then transferred to holding cups and mortality rate was recorded to four days post exposure.
Results
The results show good efficacy of SumiShield 50WG on banco and cement substrates after twelve month in experimental huts and eight months on the field area. In Bandaffassi and Tomboronkoto, mortality rate of susceptible strain was always more than 80% after eight months.
Conclusion
The use of this new formulation of clothianidin belonging the neonicotinoid class does not yet show resistance on malaria vectors countries in Africa
In silico analysis of capsid and rep proteins in CRESS DNA viruses
Authors: Selvaraj Karthiga , Yadhav Yagavi , Ramasamy Selvarajan , Venkataraman Sangita 
Published: August 2023

Abstract
The circular rep-encoding single-stranded DNA viruses (CRESS DNA viruses) are among the smallest, with 2–6 kb ssDNA genomes that encode for a coat protein (C) and a replication protein (R). To comprehend the complexity and divergence of the C and R proteins, we have created predictive structural models of representative viruses infecting unique hosts from each family using the neural network-based method AlphaFold2. The Cs exhibit substantially more diversity than Rs, and while they retain the fundamental jelly-roll fold, the loops and amino-terminal ends have undergone significant conformational shifts. The Rs, on the other hand, have minimal diversification and involve alterations only to the central linker and C-terminal domains. A phylogenetic analysis of the C and R proteins based on their structures indicates evolutionary variances and components that might have aided adaption to diverse hosts and vectors. Our study also highlights the conservation of structural features involved in the interaction of R with the conserved intergenic region of the genome. The versatility of the central linker domain may be crucial for establishing interactions of R with multiple protein partners, including C.
Near Hemispherical Passive Beam Steering using 3D Modified Partial Maxwell Fisheye Lens
Authors: Fessaras Theodore , Nicholson Kelvin , Mirotznik Mark 
Published: August 2023

Abstract
This study presents the design and fabrication of an ultra-wide-angle beam-steering graded dielectric lens using innovative principles and additive manufacturing techniques. The lens demonstrates remarkable performance with a boresight realized gain of 23 dBi and notable beam steering capabilities spanning from -80° to 80°, accompanied by a modest gain roll-off of less than 10 dB over a wide frequency range. The lens design is based on an optimized modification of the Partial Maxwell Fisheye (PMFE) lens, resulting in a fully manufacturable 3D dielectric lens. Additive manufacturing, specifically Fused Filament Fabrication (FFF), is employed to overcome challenges associated with fabricating the lens's complex geometry. Material constraints are addressed by utilizing a high dielectric filament to meet the design requirements. The design methodology incorporates a novel polar space-filling curve (PSFC) to achieve the desired gradient profile. Rigorous simulations and experimental verification confirm the effectiveness of the design, representing the first 3D implementation of a PMFE-type lens. This research demonstrates the feasibility and potential applications of an ultra-wide-angle passive beam-steering dielectric lens.
Association between weight control behaviors and health-related quality of life in Korean adults
Authors: Park Sat Byul 
Published: August 2023

Abstract
Background: Increasing evidence suggests that obesity is associated with a reduction in health-related quality of life (HRQL). HRQL is a widely used measure for assessing the degree of impairment in psychosocial and physical functions associated with disease states. This study aimed to examine the association between weight control behaviors and HRQL in Korean adults without diabetes.
Methods: This study used data from the 2017 Korea National Health and Nutrition Examination Survey. A total of 3,575 adults without diabetes were selected and divided into four groups according to their weight control behavior: trying to lose weight, trying to maintain weight, trying to gain weight, and not trying to control weight. HRQL was evaluated using the EuroQol five-dimensional (EQ-5D) questionnaire. The EQ-5D consists of five multiple-choice questions and one subjective health level. Health status was determined with respect to the following five dimensions: mobility, self-care, usual activities, pain/discomfort, and anxiety/depression. The participants were asked to choose one of the following three responses: “No problem at all,” “There are some problems,” or “There are many problems.”
Results:A statistically significant difference in the EQ-5D index score was observed among the four groups before and after controlling for age, sex, and body mass index (BMI). ANOVA and Tukey’s post-hoc test showed that the “trying to maintain weight” group had the highest EQ-5D index score. The “trying to lose weight” group had the second highest EQ-5D index score, followed by the “not trying to control weight” group, whereas the “trying to gain weight” group had the lowest EQ-5D index score (p-value compared with the “trying to maintain weight” group = 0.053, 0.001, and 0.002, respectively).
Conclusions: Participants trying to maintain their weight had the best quality of life. Individuals who are interested in their health tend to have a high quality of life. Conversely, individuals who are not interested in their health, such as those who are not trying to control their weight, tend to have a low quality of life. Furthermore, underweight individuals are against health promotion.
Remnant wild kenngoor (Marsupialia: Dasyuridae: Phascogale calura) populations retain genetic connectivity and genetic diversity in a highly fragmented landscape
Authors: de Visser Rhiannon S.J. , Hall Michelle , Ottewell Kym , Pierson Jennifer C. , Sanders Angela , Friend J. Anthony , Berry Laurence , Hogg Carolyn , Catullo Renee A. 
Published: August 2023

Abstract
Kenngoor (Phascogale calura) persist in < 1% of their original distribution, occupying highly fragmented remnant habitat in south-west Western Australia, with very little known of the genetic health of remaining wild populations. Recently, the species has been translocated to managed reserves to improve its conservation. Understanding genetic structure and patterns of genetic diversity is crucial to inform conservation translocations for species recovery. This study aims to 1) assess genetic structure and genetic diversity across remaining wild populations, 2) assess long-term genetic outcomes of a mixed-source wild-to-wild translocation, and 3) estimate global effective population size. We sequenced 209 samples from 13 fragmented wild populations were sequenced using reduced representation sequencing. An isolation by distance model best explained genetic structure across the wild populations, with evidence of fine scale divergence of two northern populations. Allelic richness and autosomal heterozygosity measures indicated that diversity is spread uniformly across populations, and no populations showed signs of inbreeding or strong genetic drift. The mixed-source translocation has retained the diversity of the wider species ten years post-translocation. Overall, our results suggest that connectivity between wild populations has largely been maintained and that no population is in poorer genetic health, despite the highly fragmented nature of remnant kenngoor habitat. Future translocations should aim to represent a mixture of genetically divergent populations to maintain the diversity present at the species level. Ongoing conservation management will be required to ensure the long-term viability of the species in this fragmented landscape.
miR-22 original mesenchymal stem cells exosome regulate Twist1/CADM1 Axis inhibiting proliferation, migration and invasion in osteosarcoma
Authors: Ruan Qing , Wang Cuijie , Wu Yuntao , Zhu Qingsan 
Published: August 2023

Abstract
Background
This study aims to the function of miR-22 original mesenchymal stem cells (MSC) on osteosarcoma (OS) proliferation, migration and invasion.
Method
Bio-informatics analysis including GEO2R analysis, Gene Ontology analysis, integration analysis were used to confirmed the target genes (miR-22, Twist1, CADM1) in OS. RT-qPCR and western blotting confirmed the different expression of miR-22, Twist1, CADM1 in OS tissues, MG63 and Saos cell lines. MTS assay, CCK8 assay, colony forming assay, EdU assay were performed to detect the proliferation effect of miR-22 on MG63. Transwell migration assay, transwell invasion assay, wound healing assay were used to verify the migration and invasion effect of miR-22 on MG63. Luciferase reporter assay confirm the binding sites between miR-22 and Twist1.
Results
RT-qPCR confirmed miR-22 and CADM1 downregulated and Twist1 upregulated in OS tissues, MG63 and Saos. Exosome original MSC labeled with PKH-26 could be uptake by MG63, which upregulated the expression of miR-22 in MG63. High expression of miR-22 in MG63 inhibited proliferation, migration and invasion, which could be rescued by Twist1. Dual luciferase reporter analysis confirmed Twist1 was a target of miR-22. Exosome modified with miR-22 mimic inhibit proliferation, migration and invasion more efficient than exosome original MSC.
Conclusions
miR-22 cargo in exo-MSC could uptake by MG63 and supply MG63 with miR-22, which inhibit MG63 proliferation, migration and invasion through targeting Twist1.
Research on Automotive Scrap Metal Classification Method Using Laser-Induced Breakdown Spectroscopy and Two-Step Clustering Algorithm
Authors: Lin Jingjun , Dai Panyang , Che Changjin , Lin Xiaomei , Li Yao , Yang Jiangfei , Huang Yutao , Ren Yongkang , Zhen Xin 
Published: August 2023

Abstract
In the recycling of scrap metal, the establishment of the classification database of recyclables has the advantages of fast classification speed and high analysis accuracy. However, the classification and recycling of unknown samples become highly significant due to the extensive variety of standard metal samples and the challenges in obtaining them. In this study, a method for multi-element classification of automotive scrap metals in general environmental conditions was achieved by utilizing Laser-Induced Breakdown Spectroscopy (LIBS) and Two-Step Clustering Algorithm (K-means, Hierarchical Clustering). The two unsupervised learning algorithms were employed to cluster the LIBS spectral data of 60 automotive scrap metal samples rapidly and hierarchically. Three rare metal elements and three elements for distinguishing metal categories were selected to meet the recycling requirements. After applying the MSC (Multiplicative Scatter Correction) to the spectral data for calibration, the initial clustering clusters were determined using the DB index, CH index, and silhouette coefficient. Then, the Kruskal-Wallis test was conducted on each cluster to check the significance. And the clusters that failed the test were split and reclustered until all clusters met the significance criterion (α=0.05). The accuracy of the proposed method for classifying the collected automotive scrap metals reached 97.6%. This indicates the great potential of this method in the field of automotive scrap metal classification.
Newborn Cystic Fibrosis Diagnosis Made Accurate and Efficient with Machine Learning to Reduce False Positives in IRT-Trypsinogen Immunoreactive Screening Program
Authors: Custódio Paulo Rogério Siqueira , Klausner Virginia , de Almeida Rainara Moreno Sanches 
Published: August 2023

Abstract
This paper presents a methodology for developing a predictive model using random forests to identify true positive cases of cystic fibrosis in neonatal screening, aiming to improve early detection and care for patients. The current heel prick test used in Brazilian neonatal screening has a high incidence of false positives, leading to unnecessary anxiety and medical interventions for patients and their families. Our methodology, developed using synthetic data and varied model parameters, showed promising results in improving the sensitivity of the model for identifying true positive cases. Our methodology prioritizes sensitivity in the model and uses general indices from the neonatal screening laboratory APAE in São Luís do Maranhão. In conclusion, our approach offers a promising avenue for developing a predictive model for cystic fibrosis in neonatal screening and highlights the potential benefits of improving early detection and care for patients.
Experiences of Stigma, Discrimination, and Violence and their Impact on Mental Health of Health care Workers during the COVID-19 Pandemics
Authors: Janouskova Mirka , Pekara Jaroslav , Kucera Matej , Cermakova Pavla , Seblova Jana , Wolfová Katrin , Kuklova Marie , Seblova Dominika 
Published: August 2023

Abstract
Background
Health care workers have been exposed to COVID-19 more than other professions, which may have caused stigmatization towards them, possibly impacting their mental health. This study aimed to explore experiences of health care workers with stigmatization, discrimination, and violence and their association with psychological distress and depression.
Method
This cross-sectional study conducted a survey among total of 4,938 health care workers in years 2020 (n = 1,731), 2021 (n = 1,809) and 2022 (n = 1,398). We used a combination of quantitative logistic regression and qualitative content analysis of open-ended questions.
Results
Experiencing stigmatization and discrimination was the highest at the first peak of the pandemic and then slightly decreased, whereas experiencing violence was highest in the latest data collection. Potential predictors of stigmatization, discrimination, and violence included exposure to COVID-19, demographic variables (gender, age), and profession. Participants with higher experience with stigmatization, discrimination, and violence were more likely to report psychological distress and depressive symptoms. Particularly, this association was highest for psychological distress in the early wave (odds ratio = 2.97, 95% confidence interval 2.27–3.88) and for depression in the middle wave (odds ratio = 2.78, 95% confidence interval 2.12–3.64).
Conclusion
Attention should be paid to destigmatization of contagious diseases and prevention of discrimination, violence, and mental health problems, both within workplaces and among the public.
Benefits and Limitations of a Dual Faculty Neurosurgeon Approach to Resection of Pediatric Craniopharyngioma
Authors: Karsten Madeline B. , Slingerland Anna L. , Riordan Coleman P. , Smith Edward R. , Fehnel Katie P. 
Published: August 2023

Abstract
Purpose:
The utility and safety of including two neurosurgeons for tumor resections is unknown. This study compares outcomes among pediatric patients with craniopharyngiomas operated on with a dual or single surgeon approach (DSA, SSA).
Methods:
A single-center review identified all craniopharyngioma transsphenoidal or craniotomy resections from 2000-2020. Surgical years of experience (YOE) and rates of 5-year reoperations, complications, recurrence, postoperative radiotherapy were analyzed.
Results:
26 transsphenoidal and 68 craniotomies were identified among 62 patients. Eleven transsphenoidal (42.3%) utilized DSA and 15 utilized (57.7%) SSA. Eight craniotomies (11.8%) were DSA and 60 (88.2%) were SSA.
The surgeon for SSA transsphenoidal procedures had a median of 10.7 YOE (IQR: 9.9-13.7) versus 6.6 (IQR: 2.7-16; p = 0.058) for the lead surgeon in DSAs. The co-surgeon in transsphenoidal DSAs had a median of 27 YOE (IQR: 11.8-35.7).
The surgeon for SSA craniotomies had a median of 19.3 YOE (IQR: 12.1-26.4) versus 4.5 years (IQR: 1.3-15.3; p = 0.017) for the lead surgeon in DSA cases. The co-surgeon in DSA craniotomies had a median of 23.2 YOE (IQR: 12.6-31.4).
Case complexity was similar across transsphenoidal groups. DSA transsphenoidal resections had fewer complications (18% DSA vs. 33% SSA), reoperations (45% vs. 53%), and radiation therapy (9.1% DSA vs. 33% SSA) than SSA.
Conclusion:
Lead surgeons in DSAs are frequently junior surgeons while SSAs typically employ senior surgeons. Outcomes did not significantly differ between DSA and SSA, but there was a trend towards improved outcomes for DSA transsphenoidal cases. Mentorship through DSAs does not seem to negatively affect patient care.
Formoterol improves the deficits of mitochondrial homeostasis including dynamic and transport in human SH-SY5Y neuroblastoma cells induced by mitochondrial UQCRC1 mutation in human neuronal SH-SY5Y cells via a β2-adrenoreceptor activation
Authors: Chang Jui-Chih , Chang Huei-Shin , Chao Yi-Chun , Huang Ching-Shan , Lin Yong-Shiou , Lin Chin-Hsien , Wu Zhong-Sheng , Chang Hui-Ju , Liu Chin-San , Chuang Chieh-Sen 
Published: August 2023

Abstract
Formoterol, a β2-adrenergic receptor (β2AR) agonist, has been showed to support mitochondrial biogenesis in various diseases. However, its efficacy is controversial in Parkinson’s disease (PD) and its regulatory mechanism of mitochondrial homeostasis remains unclear. This study used a cell model of human reductase core protein (UQCRC1) variants in familial parkinsonism, which expressed mitochondrial dysfunction and dynamic imbalance, to explore the therapeutic effects of formoterol and their underlying mechanism. The results indicated that formoterol treatment for 24 h improved cell proliferation and neural cell activity and afforded neuroprotection against oxidative-stress-induced cell death. Furthermore, mitochondrial function, including mitochondrial DNA copy number, respiratory rate, and complex III activation, was comprehensive recovered, as was the dynamic balance of fusion/fission events. Formoterol treatment properly induced mitochondrial fission and reduced the extensive hypertubulation observed. The underlying mechanism of action of the drug may proceed through the restoration of the ERK signal and the inhibition of Akt overaction in mutant cells, thus significantly upregulating the mitochondrial fission protein Drp-1, including its phosphorylation at Ser616 and dephosphorylation at Ser637, as well as Pink-1, in contrast with the fusion protein Mfn2. Moreover, formoterol contributed to the segregation of healthy mitochondria for distribution and mitochondrial transport, as our data revealed the facilitation of mitochondrial anterograde movement and mobility by the drug, to normalize mitochondrial distribution in mutant cells. This study provided preliminary evidence that formoterol offers neuroprotection and acts as a balance regulator of mitochondrial dynamic to improvement of mitochondrial homeostasis, which renders it a promising therapeutic candidate for PD.