bluuebunny/crossref_metadata_2025_split huggingface dataset:
The novel HS-mimetic, Tet-29, regulates immune cell trafficking across barriers of the CNS during inflammation
Authors: Peck Tessa , Davis Connor , Lenihan-Geels Georgia , Griffiths Maddie , Spijkers-Shaw Sam , Zubkova Olga V , Flamme Anne Camille La 
Published: August 2023

Abstract

Background
Disruption of the extracellular matrix at the blood–brain barrier (BBB) underpins neuroinflammation in multiple sclerosis (MS). The degradation of extracellular matrix components, such as heparan sulfate (HS) proteoglycans, can be prevented by treatment with HS-mimetics through their ability to inhibit the enzyme heparanase. The heparanase-inhibiting ability of HS-mimetics has been investigated in various cancers but their efficacy in neuroinflammatory models like MS has not been realised. This study investigates the use of a novel HS-mimetic, Tet-29, in an animal model of MS.
Methods
Neuroinflammation was induced in mice by experimental autoimmune encephalomyelitis, a murine model of multiple sclerosis. In addition, the BBB and choroid plexus were modelled in vitro using transmigration assays, and migration of immune cells in vivo and in vitro was quantified by flow cytometry.
Results
We found that Tet-29 significantly reduced lymphocyte accumulation in the central nervous system which, in turn, decreased disease severity in experimental autoimmune encephalomyelitis. The disease-modifying effect of Tet-29 was associated with a rescue of BBB integrity, as well as inhibition of activated lymphocyte migration across the BBB and choroid plexus in transwell models. In contrast, Tet-29 did not significantly impair in vivo or in vitro steady state-trafficking under homeostatic conditions.
Conclusions
Together these results suggest that Tet-29 modulates, rather than abolishes, trafficking across central nervous system barriers.
Depletion of chitinase-3-like protein 1 (CHI3L1) in glioma cells restraints tumor growth and affects neovasculature in intracranial murine gliomas
Authors: Kaminska Bozena , Cyranowski Salwador , Ghosh Mitrajit , Wojtas Bartosz , Zawadzka Malgorzata , Baluszek Szymon , Swatler Julian , Wojnicki Kamil , Gielniewski Bartlomiej 
Published: August 2023

Abstract
Chitinase-3-like protein 1 (CHI3L1) is a secreted, non-enzymatic glycoprotein that binds proteins and carbohydrates and interacts with cell-surface and extracellular-matrix proteins, proteoglycans, and polysaccharides. Multiple interacting partners of CHI3L1 make dissection of its functions challenging. While many studies reported an upregulation of CHI3L1 mRNA/protein in various tumors, its exact roles in tumorigenesis remain elusive. We performed a comprehensive analysis of CHI3L1 expression in multiple public datasets including TCGA and single-cell RNAseq datasets to determine the cellular source of CHI3L1 expression in gliomas. The highest CHI3L1 mRNA/protein levels were detected in glioblastoma (GBM), a high-grade diffusive brain tumor. CHI3L1 knockout in human U87-MG glioma cells grossly affected transcriptional profile and in vitro invasiveness of these cells and strongly reduced the growth of intracranial U87-MG tumors in athymic mice. Remarkably, CHI3L1 knockout in glioma cells resulted in normalization of tumor vasculature and diminished infiltration of glioma-associated myeloid cells. Mechanistically, CHI3L1 depleted cells had reduced MMP2 expression/activity, which was associated with reduced invasion; and downregulated SPP1 (osteopontin), a crucial factor driving myeloid cell accumulation in GBM. Altogether, we demonstrate that CHI3L1 is a key player in GBM progression, and its targeting represents a novel strategy to treat GBM patients.
Monitoring of sewer systems: optimization model based on search space reduction through a topological metric
Authors: Simone Antonietta , Cristo Cristiana Di , Fecarotta Oreste , Morani Maria Cristina 
Published: August 2023

Abstract
Sewer monitoring is a very relevant and current topic, also supporting management and maintenance activities, with interventions aimed at reducing the impacts on receiving water bodies as much as possible. Moreover, wastewater monitoring is crucial also for epidemiological purpose with the diffusion of the wastewater-based epidemiology (WBE), as emerged during the Covid pandemic for individuating the virus presence on a community-level. The planning of monitoring systems, allowing for the identification of the number of sensors and their positioning in the network, have been delegated for a long time to the judgment of expert technicians, sometimes resorting to trial-and-error strategies. Only recently, the impellent need for controlling such systems, mainly with respect to the presence of illicit spills and the spread of epidemics, have fostered the proposal of further increasingly efficient monitoring strategies with contained computational effort. In this perspective, the present paper proposes a novel two-step strategy for the optimal design of monitoring system in sewer systems. The first step aims at reducing the system's search space using a relevance-based topological metric, which provides a ranking of the most suitable nodes to host sensors. The second step acts on the reduced search space through an optimization procedure aiming at searching for the best location of a fixed number of sensors, with specific threshold value, in order to maximize the reliability of the monitoring system in detecting target substances or contaminant. The results demonstrate that shrinking the search space considerably reduces the computational times providing very reliable solutions.
Tribological properties investigations of short glass fiber, graphite, PTFE and B 4 C reinforced epoxy composites under oil lubrication conditions
Authors: Xiong Sang , Wu Chao 
Published: August 2023

Abstract
This study investigates the tribological performance of epoxy (EP) composites reinforced with short glass fibers (SGF), graphite, PTFE, and B4C nanoparticles, using oil lubrication. The results demonstrate that the addition of SGF significantly reduces wear and friction in the EP matrix. In contrast, the incorporation of B4C nanoparticles and other solid lubricants does not have a significant effect on friction and wear. The remarkable tribological properties observed in the SGF-reinforced EP composites can be attributed to the superior bearing capabilities and wear durability of SGF. These fibers effectively withstand the load and exhibit excellent durability during sliding, resulting in reduced wear and friction. To further understand the underlying mechanism, a comprehensive evaluation of the nanostructure of the tribofilm was conducted. The formed tribofilm acts as a protective layer during sliding, preventing direct contact between the sliding pairs and reducing the occurrence of boundary and mixed lubrication conditions. This protective film significantly impacts the performance of the tribological system. By preventing direct rubbing, the tribofilm minimizes wear and friction, thereby enhancing the tribological properties of the SGF-reinforced EP composites.
High vegetable consumption and regular exercise are associated with better quality of life in patients with gout
Authors: Do Hyunsue , Choi Hyo Jin , Choi Byoongyong , Son Chang-Nam , Kim Sang-Hyon , Ha You-Jung , Kim Ji Hyoun , Kim Min Jung , Shin Kichul , Kim Hyun-Ok , Song Ran , Lee Sung Won , Ahn Joong Kyong , Lee Seung-Geun , Lee Chang Hoon , Son Kyeong Min , Moon Ki Won 
Published: August 2023

Abstract
Background: The Gout Impact Scale (GIS), a part of the Gout Assessment Questionnaire 2.0, is used to measure gout-specific health-related quality of life (HRQOL). Recently, GIS was translated into Korean and validated for Korean patients. Several studies on factors affecting HRQOL of patients with gout have been conducted, but few have focused on lifestyle factors. This study aimed to investigate the correlation between lifestyle habits and HRQOL in Korean patients with gout.
Methods: For this study, we used data from the aftermath of Urate Lowering TheRApy in gout (ULTRA) registry, a prospective cohort of Korean patients with gout treated in multiple centers nationwide since September 2021. Participants had to be 18 years or older and meet the 2015 gout classification criteria. They were asked to fill out the GIS questionnaire in terms of their lifestyle from November 2021 to March 2023.
Results: This study included 232 patients. The mean (± standard deviation) age was 55.1 ± 17.7 years, and 90.5% of patients were men. The ‘gout concern overall’ score in the GIS was significantly lower in patients who exercised less frequently and consumed soft drinks and meat, and the ‘well-being during attack’ score was significantly lower in patients who more frequently consumed vegetables and exercised. The frequency of vegetable consumption had a negative linear relationship with the ‘well-being during attack’ and ‘gout concern during attack’ scores. The frequency of exercise had a negative linear relationship with the ‘gout concern overall’ and ‘gout concern during attack’ scores.
Conclusion: Certain GIS subscale scores were related to specific lifestyle habits in Korean patients with gout. Patients are encouraged to more frequently consume vegetables and exercise to improve HRQOL.
Efficient Electrocatalytic Reduction of CO2 to CO via Mechanochemical Synthesized Copper-based Composite Metallic Oxide Catalyst
Authors: Liu Hai , Song Tao , Wang Yimin , Zhang Xiaoli , Li Cui , Liu Tianxia , Yuan Zhen 
Published: August 2023

Abstract
Electrocatalysis serves as a highly effective approach to both mitigate greenhouse gas emissions and produce high-value chemicals. Copper-based catalysts have garnered considerable attention due to their immense potential in this domain, improving the selectivity and activity through optimizing preparation strategies is of paramount importance. In this study, mechanochemical method was first used for preparing copper-based composite metallic oxide electrocatalysts. Spherical CuO, Sn-CuO, and Sn-In-CuO catalysts were prepared and their electrochemical carbon dioxide reduction performance was evaluated. Among them, the Sn-In-CuO catalyst demonstrated the best performance in reducing carbon dioxide to carbon monoxide products. Within the potential range of -0.6 V to -1.1 V vs. RHE, the Faradaic efficiency of carbon monoxide product was consistently above 93.56%, with a maximum Faradaic efficiency of 96.11% achieved at -0.9 V vs. RHE. Sn-In-CuO also exbibts good stability with high Faradaic efficiency of carbon monoxide above 87.97% for a duration of 6 hours under the potential of -0.6 V vs. RHE in a 0.1 M KHCO3 electrolyte. The excellent performance is speculated to be attributed to the generation of a large number of defects and the introduction of metal doping, which increases the number of active sites through the mechanochemical method.
High carriage of plasmid-mediated quinolone resistance (PMQR) genes by ESBL-producing and fluoroquinolone-resistant Escherichia coli recovered from animal waste dumps
Authors: Joel Elizabeth O. , Akinlabi Olabisi C. , Olaposi Adedolapo V. , Olowomofe Temitayo O. , Adekanmbi Abimbola O. 
Published: August 2023

Abstract
There have been a rapid rise in the consumption of quinolones in human and veterinary medicine recently. This has contributed in no small measure to the rising incidence of quinolone resistance in bacteria. This study investigated the antibiotic resistance and carriage of plasmid-mediated quinolone resistance (PMQR) determinants by ESBL-producing E. coli obtained from the animal waste dumps of an agricultural farm. Isolation of ESBL-producing E. coli from the animal waste samples was done on CHROMagar ESBL, while presumptive isolates were picked and identified using molecular method (detection of uidA gene). Susceptibility to a panel of ten antibiotics was done using disc diffusion method, and detection of PMQR genes (qnrA, qnrB, qnrS, aac(6')-lb-cr, qepA and oqxAB) was done using primer-specific PCR. A total of twenty-five ESBL-producing E. coli was obtained from the cattle (6), piggery (7) and poultry (12) waste dumps of the farm. There was 100% resistance by the isolates to cefpodoxime, cefotaxime, enrofloxacin, trimethoprim-sulfamethoxazole and penicillin, while no resistance was observed to amoxicillin-clavulanate and imipenem. The resistance by the isolates to ceftazidime and streptomycin was 24% and 48% respectively. The frequency of detection of PMQR genes in the isolates was: qnrA (96%), qnrB (88%), qnrS (88%), aac(6')-lb-cr (80%), qepA (80%) and oqxAB (96%). This findings showed a high level of antibiotic resistance and PMQR genes in the ESBL-producing E. coli in this study; suggesting that animal waste dumps in agricultural farms could be a budding ‘hotspot’ for antibiotic resistant bacteria and resistance genes.
Correlation between LAMA3 and liver metastasis in pancreatic ductal adenocarcinoma
Authors: Xing Yueyi , Jing Xue , Qing Gong , Jiang Yueping 
Published: August 2023

Abstract
Purpose
The grave mortality rate of Pancreatic ductal adenocarcinoma (PDAC) is primarily due to metastasis. The objective of this investigation was to elucidate the role of LAMA3 in liver metastasis of PDAC, gauge its prognostic implications, and offer insights for therapeutic intervention in PDAC management.
Methods
We extracted information related to LAMA3 expression levels and associated clinicopathological parameters from TCGA and four GEO datasets. Clinicopathological analysis was conducted using UALCAN, while the Kaplan-Meier plotter was enlisted for evaluating LAMA3's prognostic impact in PDAC. Furthermore, we retrospectively harvested clinicopathological data and tissue specimens from 117 surgically treated PDAC patients at the Affiliated Hospital of Qingdao University. Employing tissue immunohistochemistry, we assessed LAMA3 expression, investigating its correlation with clinicopathological traits, clinical outcomes, and hepatic metastasis.
Results
(1) An amplified expression of LAMA3 was discerned in PDAC tissue compared to normal tissue in TCGA and GEO databases (all P < 0.001). High expression of LAMA3 is associated with poor OS and RFS of patients with PDAC (all P < 0.05). (2) Clinically, LAMA3 expression was significant enhanced in PDAC tissues compared to adjacent tissues (P < 0.001). (3) Tumor tissues from PDAC patients exhibiting liver metastasis had higher LAMA3 expression than those devoid of liver metastasis (P = 0.005). High LAMA3 expression was correlated with large tumor size (P = 0.007), and TNM stage (P = 0.002). (4) LAMA3 expression were independently associated with liver metastasis. (5) Both LAMA3 expression (P = 0.004) and liver metastasis (P = 0.001) were independent predictive factors for OS.
Conclusion
The expression of LAMA3 was elevated in the PDAC and it was a predictor for prognosis in PDAC patients. LAMA3 is an independent risk factor for liver metastasis in PDAC as well.
Metabolic secondary systemic insults among newborns with encephalopathy and their outcome: a descriptive single-center cohort study
Authors: Garnaud Hélèna , Soreze Yohan , Blondiaux Eléonore , Kayem Gilles , Chollat Clément , Leger Pierre-Louis , Rambaud Jerome , Guilbert Julia , Guellec Isabelle 
Published: August 2023

Abstract
Purpose: To evaluate the frequency of metabolic secondary systemic insults (mSSI) and its association with mortality in newborns with encephalopathy.
Methods
This single-center observational study of neonatal encephalopathy (NE) in patients in 2018–2020 defined mSSI as at least one abnormal finding for any of glucose, sodium, and/or carbon dioxide from routine blood samples during the first 3 days of life. Thresholds were hypoglycemia, <2 mmol/L; hyperglycemia, >10 mmol/L; hyponatremia, <130 mmol/L; hypernatremia, >150 mmol/L; hypocapnia, <30 mmHg; and hypercapnia, >70 mmHg. The cumulative number of mSSIs was the sum of these six types. The primary outcome was in-hospital mortality.
Results
Among the 109 newborns included, 22% presented no mSSI, 35% one type, 28% two, 11% three, and 4% four. The cumulative number of mSSIs was significantly (p<0.01) associated with the 5-minute Apgar score and the severity of hypoxic-ischemic encephalopathy; 8.3% of infants with no mSSI, 24.3% with one, 42.3% with two, and 53.8% with three or more mSSIs were graded Sarnat III (p<0.01). Mortality was significantly (p = 0.009) associated with the cumulative number of mSSI types: 0/24 patients with no mSSI, 2/38 (5.3%) patients with one type, 3/31 (9.7%) with two types, and 5/16 (31.3%) with three or more types.
Conclusion: Among the neonates hospitalized for NE, mSSI concerned 80%. The cumulative number of mSSI types was significantly associated with mortality. Further studies are needed to evaluate the prognostic impact of controlling these mSSIs.
Convergence Analysis of Picard Thakur Hybrid Iterative Scheme for α-Nonexpansive Mappings in Uniformly Convex Banach Spaces
Authors: Zhao Cangmei , Ahmad Khushdil , Nazar Nazia , Shabbir Khurram 
Published: August 2023

Abstract
In this paper, we investigate the convergence behavior of fixed points for generalized α-nonexpansive mappings through Picard-Thakur hybrid iterative scheme. We establish both weak and strong convergence results for generalized α-nonexpansive mappings in uniformly convex Banach space. Through a numerical example, we demonstrate that the Picard-Thakur hybrid iterative scheme converges faster than other well-known schemes. Moreover, we present a data dependence result and provide a numerical example for the data dependence. The results obtained are extend and generalized the corresponding results in the existing literature.
2010 Mathematics Subject Classification. 47H09, 47H10, 24H25.
Curcumin synergistically improves mitochondrial dynamics and myocardial cell bioenergy after sepsis through the SIRT1-Drp1/PGC-1α pathway
Authors: Liu Ruixue , Hao Shuai , Hou Dongyao , Tian Hongni , Hong Weilong , Huang He , Duan Chenyang 
Published: August 2023

Abstract
Septic cardiomyopathy (SCM) is associated with an imbalance in mitochondrial quality and high mortality rates, and an effective treatment has not been developed to date. Curcumin provides antioxidant, anti-inflammatory, cardiovascular, and mitochondrial protection. However, it has not been confirmed to improve cardiac dysfunction in sepsis and reduce abnormal inflammatory responses by improving mitochondrial function. Herein, we explore novel mechanisms by which curcumin improves SCM using an in vivo male C57BL/6 mice sepsis model and in vitro HL-1 cells stimulated with lipopolysaccharide. Curcumin’s effects on sepsis-induced cardiac dysfunction, inflammatory responses, and mitochondrial quality of cardiac cells were observed using qPCR, western blotting, echocardiography, and transmission electron microscopy. Curcumin-activated sirtuin 1 (SIRT1) increased the expression of mitochondrial biogenesis-related genes PGC-1α, TFAM, and Nrf2, reduced dynamin-related protein 1 (Drp1) translocation from the cytoplasm to mitochondria, and restored the mitochondrial morphology and function in cardiac cells, thus protecting heart function after septic shock and alleviating the effects of SCM. SIRT1 knockdown reversed the protective effects of curcumin on mitochondria. Curcumin promotes mitochondrial biogenesis and inhibits mitochondrial fragmentation by activating SIRT1, thereby improving the mitochondrial quality and reducing oxidative stress in cardiomyocytes and sepsis-induced cardiac dysfunction. These findings provide new evidence supporting the use of curcumin to treat SCM.
Associations Between Physical Activity, Mental Health Concerns, Eating Disorder Symptoms, and Emotional Intelligence in Adolescent Athletes in the Post COVID-19 Pandemic Era
Authors: Firouzjah Morteza Homayounnia , Hower Heather , Shahrbanian Shahnaz 
Published: August 2023

Abstract
Background: COVID-19 significantly disrupted the routines of school sports for adolescent athletes. In the post-pandemic era, athletes’ levels of physical activity (PA) may remain low, with ongoing mental health (MH) concerns, and exacerbated eating disorder (ED) symptoms.
However, factors such as emotional intelligence (EI; the ability to understand and regulate emotions in oneself and others), may serve as a coping mechanism for altered PA, MH, and ED.
Methods: Participants of the study included 315 Iranian adolescent athletes residing in Mazandaran province (one of the most affected areas of Iran during COVID-19), who played in 1 of 10 sports post quarantine restrictions (January 2022-January 2023). PA was assessed by the Physical Activity Stages of Change Questionnaire (PASCQ), MH concerns were assessed by the General Health Questionnaire-28 (GHQ-28), ED symptoms were assessed by the Eating Attitudes Test-26 (EAT-26), and EI was assessed by the Schutte Self-Report Emotional Intelligence Test (SSEIT).
Results: There was a significant positive relationship between PASCQ and GHQ-28 (p = .038; as PA increased, MH increased), and a negative relationship between EAT-26 and GHQ-28 (p = -.041; as ED increased, MH decreased). Nevertheless, there was a positive relationship between SSEIT and GHQ-28 (p= .028; as EI increased, MH increased), and a negative relationship between SSEIT and EAT-26 (p = -.038; as EI increased, ED decreased).
Conclusions: The study has limitations which future research should address. However, the findings highlight the need for sports professionals working with adolescent athletes in the post COVID-19 era to monitor the status of PA, MH, and ED, and promote EI coping skills, to provide early intervention, and mitigate long-term consequences.
Older adult drug overdose: An application of latent class analysis to identify prevention opportunities
Authors: Mason Maryann , Pandya Kaveet , Lundberg Alexander 
Published: August 2023

Abstract
Background
Older adult overdose death rates have increased significantly in recent years. However, research for prevention of drug overdose death specific to older adults is limited. Our objective is to identify profiles based on missed intervention points (touchpoints) to inform prevention of future older adult unintentional overdose deaths.
Methods
We used latent class analysis methods to identify profiles of decedents aged 55 + years in the Illinois Statewide Unintentional Drug Overdose Reporting System. This system collects data on 88% of all unintentional overdose deaths in Illinois and includes data from death certificates, coroner/medical examiner, toxicology and autopsy reports. Data include decedent demographics, circumstances leading up to and surrounding the fatal overdose and details regarding the overdose. Variables in the latent class analysis model included sex, race, alcohol test result, social isolation, recent emergency department (ED) visit, chronic pain, and pain treatment.
Results
We identified three distinct decent profiles. Class 1 (13% of decedents) included female decedents who were in pain treatment, had physical health problems, and with greater likelihood of a recent ED visit before their death. Class 2 (35% of decedents) decedents were most likely to be socially connected (live with others, employed, had social or family relationships) but less likely to have recent healthcare visits. Class 3 (52% of decedents) decedents had higher social isolation (lived alone, unemployed, unpartnered), were mostly male, had fewer known physical health conditions and more alcohol positivity at time of death.
Conclusions
These profiles link to potential touchpoint opportunities for substance use disorder screening harm reduction and treatment. Class 1 members were most likely to be reachable in healthcare settings. However, most decedents were members of Classes 2 and 3 with less engagement in the healthcare system, suggesting a need for screening and intervention in different contexts. For class 2, intervention touchpoints might include screening in social settings such as senior centers given the higher degree of social connectivity. For class 3, the most isolated group, touchpoints may occur in the context of harm reduction outreach and social service delivery.