bluuebunny/crossref_metadata_2025_split huggingface dataset:
A national survey of oral maxillofacial surgeons’ and trainees’ awareness and practice regarding psychological problems associated with facial trauma
Authors: Kolawole Ogundipe Olufemi , Gbenga Akomolafe Ayodele , Francis Adejobi Adewale , Raphael Njokanma Azuka , Akinsulore Adesanmi 
Published: March 2023

Background: The psychological problems associated with facial trauma may sometimes pose additional health concerns for the injured patient. Understanding the gaps in the Oral and Maxillofacial surgeons’ (OMFS) awareness of patient in need of specialist mental health intervention is important in ensuring holistic care for the trauma patient.
Objectives: To determine the knowledge, practices and self-assessed level of competence of Nigerian Oral maxillofacial surgeons/trainees regarding psychological problems associated with facial trauma and to determine their perceived need for training in assessment of psychological consequences following facial trauma.
Methods: A cross-sectional study was conducted in which a web-based structured questionnaire was sent to Oral and maxillofacial surgeons and trainees.
Results: Majority of respondents (85.2%) had encountered psychological problems in patients who have sustained facial trauma. Half (50.6%) of OMFS / trainees had high level of knowledge regarding psychological problems associated with facial trauma; depression, anxiety, post-traumatic stress disorder, body image disorder and acute stress syndrome were the five most common trauma related psychological problems mentioned. More respondents reported low level of competence in conducting mental state examination when compared to other skill sets. In-unit counselling was reported as the method of intervention by majority (69.1 %), followed by referral to the mental health specialists (17.3%). More than one third (40.7%) of OMFS were extremely interested in undergoing additional training in the psychological assessment of patients who have sustained facial trauma.
Conclusion: Nigerian Oral and maxillofacial surgeons frequently encounter psychological problems in patients with traumatic facial injuries. Notwithstanding their perceived self-assessed low level of competence in psychological assessment of trauma patients, objective assessment revealed a relatively high level of knowledge of psychological problems that can affect the facial trauma patient with many indicating a high perceived need for additional training in the psychological assessment of facial trauma patient. There is a need for incorporating psychological assessment into the management to ensure holistic care of trauma patients.
Keywords: Maxillofacial trauma; psychological problems; Nigeria.
Ethnic spice use and perceived health benefits among women in a Suburb of the Greater Accra Region of Ghana
Authors: Asante Matilda , Asante Precious , Nkumsah-Riverson Portia , Amokao-Mensah Anna , Dogbe Yauniuck 
Published: March 2023

Background: Spices have a long history of both culinary use and health benefits. Ethnic spices are spices specific to a particular geographic location. In Ghana, there are varying perceptions and uses of ethnic spices which are mostly not documented.
Objective: To assess the use and perceived health benefits of ethnic spices among women in Accra, Ghana.
Methods: A cross-sectional study was conducted among 370 women in randomly selected churches in Accra, Ghana. A semi-structured questionnaire was used to obtain sociodemographic data and information on ethnic spice use. Chi-square test was used to determine the association between socio-demographic characteristics of participants and perceived health benefits of ethnic spice usage.
Results: The most commonly consumed spices out of the 19 spices explored in this study were onion (98.4%), garlic (97.6%), ginger (96.8%) and chili pepper (84.1%). Majority of the participants perceived ginger (74.6%) and garlic (73.8%) as having health benefits. Age (p=.001) and self-reported health status (p=0.010) were significantly associated with perceived health benefits of the spices.
Conclusion: Onion, garlic, ginger and chili pepper were the four most commonly consumed ethnic spices. Age and self-reported health status of participants influenced perceived health benefits of ethnic spice usage.
Keywords: Spices; health benefits; perception; flavour.
Experiences of LGBTI+ individuals in accessing right to health; a survey study from Turkey
Authors: Gunbegi Meltem , Sazak Uygul Elif , Turla Ahmet , Aydin Berna 
Published: March 2023

Background: In Turkey, LGBTI+s is experiencing discrimination in all areas of their lives.
Objectives: We aimed to determine the problems experienced by LGBTI+s living in Turkey in accessing their right to health.
Methods: An online questionnaire containing 37 open-ended and multiple-choice questions about respondent’s demographic characteristics, experiences in accessing and receiving health services was prepared. Between October 2018 and December 2019, the survey was disseminated via social media platforms and sent to LGBTI+ friendly institutions. 81 people responded to the survey.
Results: 43.2% had at least one chronic disease. Participants stated that 44.5% of them go to a health institution <3 times in a year. 91.4% of the participants declared that they never or rarely said their sexual identity/orientation at the health institutions, and 39.2% of them encountered negative behaviour when they did. 98.8% of the participants said that they think physicians do not have enough information about LGBTI+s.
Conclusions: The knowledge and attitude of healthcare professionals are one of the essential determinants of LGBTI+s' use of their right to health. Alienating and homophobic behaviors against LGBTI+s is the biggest problem for LGBTI+s to receive quality health care in Turkey.
Keywords: LGBTI+; health service; right to health; discrimination; access to health services.
Gender disparities in the socio-economic burden of HIV/AIDS among patients receiving care in an HIV clinic in Lagos, Nigeria
Authors: O Charles-Eromosele Titilope , J Kanma-Okafor Oluchi , O Sekoni Adekemi , O Olopade Bolatito , B Olopade Oluwarotimi , E Ekanem Ekanem 
Published: March 2023

Background: In sub-Saharan Africa, women are bearing a heavier burden than men in terms of rate of infection and socio-economic impact of HIV/AIDS. This study was aimed at assessing gender disparities in the socio-economic burden of HIV/AIDS.
Methods: This descriptive cross-sectional study was conducted among 422 HIV-positive adult patients attending an HIV clinic in Lagos, Nigeria, selected by multi-stage sampling and interviewed using a pretested, semi-structured questionnaire. Bivariate analysis was used to assess how the socioeconomic constructs differed by gender.
Results: This study revealed that females suffered more of the socio-economic consequences of having HIV/AIDS than males; cruelty and isolation were significantly higher among the females (p<0.0001), more females (50.0%) were discriminated against at the workplace compared to males (32.1%) (p=0.005), physical abuse (p=0.002) and extortion (p=0.029) were experienced by more of the females than the males. Also, the cost of care outside of antiretroviral therapy was significantly higher among the females (p= 0.002).
Conclusion: Quantifying the social and economic disparities between HIV-infected men and women has shown that the burden is by far higher among women than men. Focused interventions are therefore needed to control the spread of the disease and improve the quality of life of HIV-infected women.
Keywords: HIV/AIDS; gender disparities; women; Lagos; Nigeria.
Compliance and approach to voluntary HIV testing in a high-risk region for HIV transmission in Europe
Authors: Milic Marija , Dotlic Jelena , Stevanovic Jasmina , Mitic Katarina , Nicholson Desmond , Karanovic Andrijana , Vujacic Amila , Gazibara Tatjana 
Published: March 2023

Background: The Kosovo province is being considered as a high-risk region for the spread of HIV.
Objective: To estimate the prevalence and factors associated with HIV testing in a sample of university students from the Serbian northern Kosovo province.
Material and Methods: A questionnaire examining socio-demographic characteristics, HIV–related knowledge, attitudes towards people living with HIV (PLHIV) and HIV testing was used in data collection. A total of 1,017 students from the University of Priština temporarily seated in Kosovska Mitrovica completed the questionnaire.
Results: Only 5.4% of students have previously been tested for HIV, even though the majority (70.9%) had a positive approach to HIV testing. Factors associated with having been tested for HIV were being male and younger, having interest in HIV testing and having previous contact with PLHIV. Being more knowledgeable about HIV and having stronger positive attitude towards PLHIV, being older, receiving information about HIV through friends and special educational programs, using condom at last sexual intercourse, having positive opinion on gays/lesbians and previous contact with PLHIV were associated with positive approach to HIV testing.
Conclusion: Having a positive approach to HIV testing does not suggest that students would take the HIV test. However, students who have low HIV-related knowledge, negative approach or lack of interest in HIV testing (believing that there is no need to take it) would likely never take the HIV test. Increasing HIV-related knowledge, acceptance of PLHIV and access to testing facilities should be public health priorities to raise HIV testing rates.
Keywords: HIV testing; attitude; knowledge; University students.
Multiple myeloma with unexplained isolated anaemia in a 24year old man- a case report
Authors: Wale Aworanti Oladapo , Peter Ogundeji Sunday , Asake Adeoye Olateni , Adebola Shokunbi Wuraola 
Published: March 2023

Background: Multiple myeloma (MM) is a disease of the elderly with a median age at presentation of 70 years. It is rare to diagnose MM in individuals less than 40 years and even extremely rare in those less than 30 years of age. MM is usually suspected in those aged 50 years and above having a combination of hypercalcemia, renal insufficiency, anaemia and bone lesions. Although anaemia is a common clinical feature of MM, it is very rare that anaemia would be the only clinical presentation, hence the need to report this index case.
Case Presentation: We present a rare case of MM in a 24-year- old male who presented with only symptomatic anaemia.Investigations for the cause of anaemia, including Bone marrow aspiration cytology revealed a diagnosis of MM ISS stage II.Here, we highlighted the need to seek early haematologist consultation in investigating patients’ whose cause of anaemia is notimmediately obvious from the clinical presentation and routine laboratory investigations.
Conclusion: MM can present at a younger age with unexplained anaemia without bone pains or renal insufficiency. High level of suspicion for MM is required in young patients with unexplained anaemia
Keywords: Multiple myeloma; isolated anaemia; young patient.
Fucosterol improves palmitic acid-induced oxidative stress, lipid droplet formation and insulin resistance in liver cells by mediating Keap1-Nrf2-lipocalin 13 axis
Authors: Xiong Zhekun , Gan Liming , Sun Chuntian , Zhang Hui , He Yanshan , Su Huixia , Zheng Yiyuan , Liao Fanglian 
Published: March 2023

Purpose: To determine the possible effects of fucosterol (FST) on non-alcoholic fatty liver disease (NAFLD), and the mechanisms involved.
Methods: The NAFLD model was constructed using palmitic acid (PA) induction, and the expression of NF-E2-related factor 2 (Nrf2), lipocalin 13 (LCN13) and Keap1 were analyzed by immunoblot. The oxidative stress of hepatocytes was determined via ELISA assay. In addition, the role of FST on lipid content and metabolism were evaluated by Oil Red O staining and immunoblot, while the levels of p-AKT, p-IRS1, and p-PI3K were evaluated by immunoblot assay.
Results: The data revealed that FST significantly increased the viability of PA-induced hepatocytes, and the expression levels of Nrf2 and LCN13 (p < 0.05). Fucosterol enhanced Keap1-Nrf2 mediated LCN13 expression, and alleviated PA-induced oxidative stress by contributing to Keap1-Nrf2-LCN13 axis. In addition, it significantly reduced (p < 0.05) lipid droplet formation, promoted lipid metabolism, and lowered insulin resistance by enhancing Keap1- Nrf2-LCN13 axis.
Conclusion: Fucosterol regulates Keap1-Nrf2-mediated LCN13 to aid the ameliorate palmitic acid-induced oxidative stress, lipid droplet formation and insulin resistance in liver cells.
Ellipticine induces apoptosis and mitochondrial dysfunction in human endometriosis cell lines by activating MAPK signaling pathway
Authors: Zhang Fenyun , Wang Qianqian 
Published: March 2023

Purpose: To assess the effect of ellipticine (EPT), an alkaloid isolated from the Oleaceae family, on endometriosis, and to identify its possible mechanisms of action.
Methods: Human endometriosis-like cell lines exposed to EPT were subjected to bromodeoxyuridine/5-bromo-2´-deoxyuridine and proliferating cell nuclear antigen staining. Flow cytometry and immunoblot analyses were used to assess the effect of EPT on cell apoptosis. Mitochondrial damage was determined by JC-1 staining and immunoblotting. Immunoblot assays were performed to determine the effects of EPT on the MAPK pathway.
Results: Ellipticine inhibited the viability of human endometriosis cell lines and stimulated cell apoptosis (p < 0.01). It further induced mitochondrial damage in human endometriosis cell lines (p < 0.01). Mechanistically, EPT acted on MAPK pathway, and induced apoptosis and mitochondrial dysfunction (p < 0.01) in human endometriosis cells.
Conclusion: Ellipticine is a potential treatment strategy for the management of endometriosis. However, further exploration of this potential should be explored via in vivo studies.
Carnosic acid attenuates inflammation, oxidative stress and mitochondrial dysfunction in neurons via activation of AMPK/SIRT1 pathway
Authors: Liao Ou , Xie Keqi , Zhang Xianjie , Jiang Wencai , Li Wen , Xie An 
Published: March 2023

Purpose: To determine the effects and mechanism of action of carnosic acid (CA), a natural diterpenoid compound, on the neuroprotective roles of anesthetics.
Methods: The effects of carnosic acid (CA) on cell viability and apoptosis were evaluated by MTT assay and flow cytometry, respectively. Its effects on mitochondrial damage was assessed by JC-1 staining, while oxidative stress and inflammatory response were determined by enzyme-linked immunosorbent assay (ELISA) and immunoblot assays, respectively. Immunoblot assays were performed to evaluate the effect of CA on AMPK/SIRT1 pathway.
Results: Carnosic acid (CA) increased the survival rate of isoflurane-induced neuronal cells, but inhibited isoflurane-induced mitochondrial damage in neurons (p < 0.01). CA also suppressed isoflurane-induced oxidative stress in neurons, and inhibited isoflurane-induced neuronal inflammatory response (p < 0.01). Furthermore, CA activated AMPK/SIRT1 pathway, and also attenuated inflammation, oxidative stress and mitochondrial dysfunction in neurons induced by isoflurane (p < 0.01).
Conclusion: Carnosic acid attenuates inflammation, oxidative stress and mitochondrial dysfunction in neurons via activation of AMPK/SIRT1 pathway. Thus, it has potentials for use in the treatment of neurotoxicity
Effect of combined application of apatinib and perioperative quality nursing based on Orem’s self-care theory in patients undergoing cholangiocarcinoma resection
Authors: Li Xinsheng , Zhong Weina , Zhang Limin 
Published: March 2023

Purpose: To investigate the effect of application of perioperative quality nursing based on Orem’s self-care theory, in combination with apatinib in patients who underwent cholangiocarcinoma resection.
Methods: Ninety patients who underwent cholangiocarcinoma resection in The Fourth Hospital of Hebei Medical University from Feb. 2019 to Feb. 2020 were assigned to control group (CG) and study group (SG) based on their order of admission, with 45 patients in each group. Conventional nursing in combination with conventional treatment was applied to CG, while perioperative quality nursing based on Orem’s self-care theory was used in combination with apatinib therapy in SG patients. Comparison was made between CG and SG regarding clinical efficacy, self-care ability and quality of life (QoL, based on QLQ-C30 score).
Results : Disease control rate (DCR) and Barthel index (BI) score were higher in SG than in CG (p < 0.05). After treatment, QoL score was higher in SG than in CG (p < 0.001).
Conclusion: Implementation of perioperative quality nursing along with apatinib therapy in patients undergoing cholangiocarcinoma resection improves their QoL and enhances their self-care ability. Thus, this treatment modality has high utility and treatment potential.
MiR-493-5p alleviates lipopolysaccharide-induced inflammation in ATDC5 chondrogenic cells by targeting TAB2
Authors: Chen Xuan , Li De , Chen Mangmang 
Published: March 2023

Purpose: To investigate the effect of miR-493-5p in lipopolysaccharide (LPS) -induced ATDC5 chondrogenic cells.
Methods: The MTT assay was used to determine the viability of LPS-induced ATDC5 cells. ENCORI (starbase.sysu.edu.cn/) was used to predict the target of miR-493-5p. Quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) was used to determine miR-493-5p expression in ATDC5 controlled cells and cells treated with various combinations of LPS, negative control miRNA (NC), miR-493-5p mimic, TAB2 overexpression vector, and miR-493-5p inhibitor. qRT-PCR, while western blot was used to assess TAB2 mRNA and protein expression levels in control, LPS, LPS + NC, LPS + miR-493-5p mimic, and LPS + miR-493-5p inhibitor groups. The qRT-PCR and ELISA were used to evaluate TNF-α, IL-18, and IL-1β expressions in control, NC, LPS, LPS + NC, miR-493-5p mimic, and LPS + miR-493-5p mimic. Furthermore, the two techniques were also used determine LPS + miR-493-5p inhibitor, LPS + TAB2 overexpression, and LPS + TAB2 overexpression + miR-493-5p mimic groups.
Results: MiR-493-5p expression was downregulated in LPS-induced ATDC5 cells (p < 0.001). Overexpression of miR-493-5p increased LPS-induced ATDC5 cell viability but decreased expression of inflammatory factors (p < 0.001). MiR-493-5p targeted and inhibited TAB2 expression in LPS-induced ATDC5 cells (p < 0.001). TAB2 overexpression reversed the suppression of TAB2 by miR-493-5p in LPS-induced ATDC5 cell injury (p < 0.001).
Conclusion: MiR-493-5p alleviates LPS-induced inflammation of ATDC5 chondrogenic cells by targeting TAB2. Thus, miR-493-5p and TAB2 have potentials for use as clinical therapeutic targets for OA.
FOXN3 attenuates doxorubicin resistance of bladder urothelial carcinoma via SIRT6/PI3K/AKT/mTOR pathway
Authors: Han Yinan , Wang Shengxing , Xia Rurui , Chen Jinhuo , Zhou Bangfen 
Published: March 2023

Purpose: To investigate the effect of forkhead box N3 (FOXN3) protein on doxorubicin (DOX) resistance of urothelial carcinoma (BLCA).
Methods: Bioinformatics prediction and immunoblotting were used to evaluate FOXN3 expression in BLCA tissues and cells. The FOXN3 overexpression was achieved by cell transfection. The effects of FOXN3 on DOX resistance and cell apoptosis were determined by immunoblotting, DOX resistance assay, and flow cytometry, while immunoblotting was applied to evaluate SIRT6/PI3K/AKT/mTOR signaling activity. Finally, SIRT6 overexpression and exogenous addition of a PI3K/AKT activator were used to investigate the molecular mechanism by which FOXN3 regulates DOX resistance phenotype.
Results: The FOXN3 was downregulated in DOX-resistant BLCA tissues and cells while its overexpression attenuated doxorubicin resistance (p < 0.01). Furthermore, apoptotic cell ratio increased from 7.54 to 26.83 % in J82/DOX cells and from 6.31 to 17.89 % in T24/DOX cells (p < 0.01) after FOXN3 overexpression. Moreover, FOXN3 upregulation inhibited sirtuin 6 (SIRT6) expression and inactivated PI3K/AKT/mTOR signaling pathway. Both SIRT6 overexpression and PI3K/AKT activation abrogated the FOXN3-mediated inhibition of DOX resistance in BLCA cells.
Conclusion: The FOXN3 attenuates the DOX resistance of BLCA through SIRT6/PI3K/AKT/mTOR pathway, thus providing a promising therapeutic strategy for the management of BLCA.
LER E ESCREVER COM ESTILO: ATUAÇÃO DO PROFESSOR NO ENSINO FUNDAMENTAL II
Authors: Araújo de Paula Teófilo Eny , Barroso de Melo Leonardo 
Published: March 2023

O problema que motivou este estudo surgiu quando se percebeu que os professores da escola foco da pesquisa não possuem, como principal preocupação, o foco da leitura e escrita em sala de aula, o que deixa o ensino com deficiências, talvez por acomodações ou por possuírem pouco domínio. Baseado nisso, esse trabalho tem como objetivo geral: Analisar os professores das séries iniciais bem como os recursos que os apoiam. E como objetivos específicos: Descrever pontos relevantes que envolvem a formação do professor das series iniciais e desenvolver a leitura e a escrita numa concepção reflexiva para os alunos das series iniciais, utilizando uma discussão teórica sobre o tema. Levando isso em consideração, concluímos que a leitura e a escrita estão em toda parte, as crianças começam a entrar em contato com o mundo letrado muito cedo, haja vista a maioria dos objetos que estão a sua volta se tornam fontes inesgotáveis de estímulos direcionados à alfabetização. Finalizando esta consideração, podemos dizer que se espera que os apontamentos aqui registrados possibilitem evidenciar embriões para a qualificação dos professores que trabalham com as séries iniciais e que formam leitores e escritores, permitindo apontar reflexões para possíveis redirecionamentos. Compreendemos que a leitura deste texto, assim como de outros, irá complementar, proporcionando elementos para redimensionar os pensamentos. Criando novas ideias, como repensar, se for necessário, as atividades educativas dos professores.
ERGONOMIA EM HOMEOFFICE: CONSEQUÊNCIAS OCULARES E VISUAIS
Authors: Trentin Sonoda Rodrigo , Lobianco de Moura Karen , Dos Santos Érica Matilde 
Published: March 2023

A ergonomia por princípio básico, identifica a interação entre os processos e todos os aspectos das atividades humanas. Durante os últimos 50 anos o uso de novas tecnologias promoveu alterações notáveis nas relações de trabalho, estudo, socialização e lazer. Com a COVID-19 o enclausuramento provocado pelas políticas públicas de lockdown, levaram as empresas e instituições de ensino a remeterem os seus colaboradores e estudantes para um sistema caseiro e adaptado ao labor. Busca-se demonstrar que a falta de conhecimento dos pilares da ergonomia, das bases da saúde ocular e visual, promoveram diversos distúrbios oculares que são mensurados na atualidade em ambiente clinico e a terapêutica adaptada para o restabelecimento da função neurovisual.