bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selection and In-Vitro Potentials of Amylolytic Yeast from Several Ragi Tapai in West Sumatra Province
Authors: Yulia Inelvi , Putra Adewirli , Fitri Wiya Elsa , Putri Gusliani Eka 
Published: July 2023

Ragi Tapai is a starter used for the fermentation of cassava and glutinous rice to produce conventional fermented products, such as Tapai in Indonesia. Ragi Tapai contains several enzymes and microbes that help the Tapai fermentation process. The purpose of this study was to identify the most potential amylolytic yeast and in-vitro potential (amylolytic, cellulolytic, and fermentative). GPA, YEA, APB, CMCA, and GPACaCO3 were used as a medium for selection and characterization. The result showed that in the seven samples of Ragi Tapai West Sumatra (Padang, Padang Pariaman, Batusangkar, Padang Panjang, Payakumbuh, Solok, dan Pesisir Selatan), there was three amylolytic yeast including genera Schizosaccharomyces (1 dan 2) and 1 genus Trichosporon. In-vitro potential, showed the genera Schizosaccharomyces and Trichosporon have amylolytic, cellulolytic, and fermentative potential. Genera Schizosaccharomyces 1 has greater amylolytic potential than genera Schizosaccharomyces 2 and Trichosporon.
TINGKAT PROFITABILITAS, LEVERAGE, DAN KEBIJAKAN HUTANG TERHADAP NILAI PERUSAHAAN DENGAN KEBIJAKAN DEVIDEN SEBAGAI VARIABEL INTERVENING (SUBSEKTOR MAKANAN & MINUMAN )
Authors: Wulandari Christina Dewi , Damayanti Tri , Anggraini Aulia Rizki 
Published: July 2023

Sektor industri manufaktur merupakan tumpuan utama terhadap pertumbuhan ekonomi di Indonesia, menurunnya nilai perusahaan pada sector pertumbuhan ekonomi industri manufaktur akan berpengaruh besar terhadap perekonomian di indonesia, maka dari itu perusahaan dituntut untuk meningkatkan nilai perusahaan agar menarik minat investor Penelitian ini dilakukan untuk menganalisis pengaruh tingkat Profitabilitas, Leverage, dan kebijkan hutang terhadap nilai perusahaan dengan kebijakan deviden sebagai variabel Intervening. Objek pada penelitian ini adalah perusahaan manufaktur sektor industri barang konsumsi dengan mengunakan teknik pengambilan sampel nonprobability sampling (non-random sampling) dan metode purposive sampling. Teknik analisis yang digunakan adalah regresi linear berganda dan analisis jalur. Berdasarkan hasil penelitian, diperoleh hasil bahwa Probabilitas, leverage tidak berpengaruh terhadap kebijakan deviden. Sementara kebijakan hutang berpengaruh terhadap kebijakan deviden. Leverage, kebijakan hutang tidak berpengaruh terhadap nilai perusahaan. Sementara tingkat profitabilitas berpengaruh terhadap nilai perusahaan. kebijakan deviden tidak berpengaruh terhadap nilai perusahaan Kebijakan deviden tidak dapat memediasi leverage dan kebijakan hutang terhadap nilai perusahaan. Sementara kebijakan deviden dapat memediasi hubungan antara tingkat profitabilitas terhadap nilai perusahaan.
Pengaruh Gaya Hidup, WOM (Word Of Mouth), Dan Brand Ambassador Terhadap Keputusan Pembelian Natasha Skincare Di Semarang
Authors: Novandalina Arini , Budiyono Rokhmad , Ernawati Fidyah Yuli 
Published: July 2023

Banyaknya perusahaan yang bergerak dalam perawatan dan kesehatan kulit (skin care) dengan berbagai merek berdampak pada berbagai alternatif pilihan bagi konsumen untuk memilih salah satu diantaranya, salah satunya Natasha Skincare. Natasha Skin Care mempunyai beberapa kelebihan seperti tempat perawatan yang nyaman, layanan konsultasi gratis dan ditangani oleh dokter berpengalaman sehingga dapat mempengaruhi keputusan pembelian konsumen. Keputusan pembelian adalah kegiatan yang dipengaruhi berbagai variabel dalam memutuskan tindakan untuk memilih produk. Banyak faktor dapat mempengaruhi keputusan pembelian Natasha Skin Care diantaranya gaya hidup, WOM (word of mouth) dan brand ambassador. Penelitian ini bertujuan mengetahui pengaruh gaya hidup, WOM (word of mouth) dan brand ambassador terhadap keputusan pembelian Natasha Skin Care di Semarang
Populasi penelitian ini adalah konsumen dan teman yang melakukan perawatan atau pembelian produk Natasha Skin Care, di Semarang yang jumlahnya tidak dapat diketahui. Sampel yang digunakan sebanyak 100 konsumen. Data penelitian ini diperoleh dengan membagikan kuesioner. Data dioleh dengan menggunakan analisis regresi berganda, uji hipotesis (uji t dan uji F) dan koefisien determinasi (R2).
Hasil hipotesis 1 yang menyatakan ada pengaruh antara gaya hidup terhadap keputusan pembelian diterima (t hitung 3,782 dengan sig. sebesar 0,000 < 0,05). Hasil hipotesis 2 yang menyatakan ada pengaruh antara WOM terhadap keputusan pembelian diterima (t hitung 3,271 dengan sig. sebesar 0,001 < 0,05). Hasil hipotesis 3yang menyatakan ada pengaruh antara brand ambassador terhadap keputusan pembelian diterima (t hitung 2,958 dengan sig. sebesar 0,004 < 0,05).
Kesimpulan dari penelitian ini adalah gaya hidup, WOM dan Brand Ambassador berpengaruh terhadap keputusan pembelian konsumen di Natasha Skin Care. Sedangkan saran dalam penelitian ini adalah agar konsumen berkeingan untuk terus melakukan perawatan berulang maka sebaiknya Natasha Skin Care menawarkan berbagai paket perawatan kulit dengan harga yang lebih terjangkau atau memberi diskon harga apabila melakukan perawatan secara paket, sehingga pelanggan akan mendapatkan harga lebih murah apabila melakukan perawatan secara berulang di Natasha Skin Care.
Integrative modeling of hemodynamic changes and perfusion impairment in coronary microvascular disease
Authors: Colombo Monika , Chaudhry Palak , Oberholzer Yvonne , deMello Andrew J. 
Published: July 2023

Introduction: Coronary microvascular disease is one of the responsible factors for cardiac perfusion impairment. Due to diagnostic and treatment challenges, this pathology (characterized by alterations to microvasculature local hemodynamics) represents a significant yet unsolved clinical problem.Methods: Due to the poor understanding of the onset and progression of this disease, we propose a new and noninvasive strategy to quantify in-vivo hemodynamic changes occurring in the microvasculature. Specifically, we here present a conceptual workflow that combines both in-vitro and in-silico modelling for the analysis of the hemodynamic alterations in the microvasculature.Results: First, we demonstrate a hybrid additive manufacturing process to fabricate circular cross-section, biocompatible fluidic networks in polytetrafluoroethylene. We then use these microfluidic devices and computational fluid dynamics to simulate different degrees of perfusion impairment.Discussion: Ultimately, we show that the developed workflow defines a robust platform for the multiscale analysis of multifactorial events occurring in coronary microvascular disease.
Pharmacokinetics, pharmacodynamics, and safety of prandial oral insulin (N11005) in healthy subjects
Authors: Pan Qi , Wang Xiaoxia , Li Wenjia , Chen Xiaofeng , Zhuang Yulei , Zhou Qinghong , Huang Yuhui , Zhou Yijie , Lan Li , Wang Zhijie , Wang Wenjia , Hong Juan , Hao Wei-Hua , Yang Yu-Tsai , Guo Lixin 
Published: July 2023

AimsTo verify whether the oral insulin N11005 is administered as a prandial insulin by assessing the pharmacokinetics (PK), pharmacodynamics (PD), and safety profiles of N11005 with a short-acting biosynthetic human insulin (Novolin R) as reference.MethodsThis was a randomized, open-label, single-dose, crossover hyperinsulinemic-euglycemic clamp study in healthy Chinese male subjects. A total of 12 subjects were enrolled in the test (T) group (N11005, 300 IU, p.o.) and the reference (R) group (Novolin R, 0.1 IU/Kg, i.h.) with a washout period of 14 days. All subjects were administered on the same day of the clamp study. Glucose Infusion Rates (GIR), serum insulin, and C-peptide concentration were determined during every 8-hour clamp cycle. Trial registration: Clinicaltrials.gov identifier NCT04975022.ResultsAfter administration, the ratios of mean serum C-peptide concentration to baseline concentration in both T and R groups were lower than 50%, which confirmed the stability of the clamp platform. T group (N11005) showed a more rapid onset of action (tGIR10%max≈11 min) and a comparable duration of action to the R group, which was basically in line with the characteristics of prandial insulins. No adverse events (AEs) occurred throughout the study, which demonstrated that N11005 and Novolin R are safe and well-tolerated.ConclusionsThe PD profiles of the single-dose N11005 in the human body are similar to those of prandial insulins, with an excellent safety profile.Clinical trial registrationClinicaltrials.gov, identifier NCT04975022.
Age-specific association between thyroid autoimmunity and hypothyroidism in Chinese adults aged over 65 years: a cross-sectional study
Authors: Zhang Mengjie , Ni Wenjing , Zhang Lina , Fan Kuanlu , Sun Yu , Liu Chao , Xu Shuhang 
Published: July 2023

BackgroundThe correlation between thyroid autoimmune (TAI) disease and hypothyroidism in the elderly of different ages remains unclear. This study aimed to investigate the epidemiological characteristics of hypothyroidism, including subclinical hypothyroidism (Shypo) and overt hypothyroidism (Ohypo) in those aged ≥65 years from iodine-adequate areas and reveal the correlation between TAI and hypothyroidism in the elderly of different ages.MethodsIt was a cross-sectional study involving 2,443 subjects aged ≥65 years from two iodine-adequate areas in China by cluster sampling. They were assigned to the 65–69-, 70–79-, and ≥80-year-old age group. All subjects were surveyed by questionnaires and received physical examinations, laboratory testing, and thyroid ultrasound. Epidemiological characteristics of thyroid diseases in the elderly were compared among the three groups. Risk factors for hypothyroidism were predicted by binary logistic regression analysis.ResultsThe median urinary iodine level was 238.70 (197.00, 273.70) μg/L. Thyroid peroxidase antibody or thyroglobulin antibody positivity (11.87%) and Shypo (9.13%) were common in the elderly. The prevalence of hypothyroidism in the elderly increases with age. TAI was a risk factor for Shypo (OR, 1.94; 95% CI, 1.35, 2.80; p &lt; 0.01) and Ohypo (OR, 7.64; 95% CI, 3.40, 17.19; p &lt; 0.01) in elderly Chinese. There was an age-specific correlation between TAI and hypothyroidism in the elderly. However, a significant correlation was not identified between TAI and hypothyroidism in ≥80-year-old age group (p &gt; 0.05).ConclusionHypothyroidism, particularly Shypo, is common in the elderly from iodine-adequate areas in China. TAI serves as a risk factor for hypothyroidism in the elderly, with an age-specific correlation with hypothyroidism.
Study on the efficiency evolution trend of coordinated development of the human–earth system in minority areas of Yunnan, China
Authors: Tai Lingjuan , Lu Fen , Huang Lin , Tian Furong , Yang Yun 
Published: July 2023

IntroductionImproving the development efficiency of human–earth systems is a practical requirement for achieving high-quality regional development.MethodsThe article designs a data envelopment analysis (DEA) model under the constraints of coordinated development degree from the perspective of the interaction of natural, economic and social systems to evaluate the development efficiency of human–earth systems and uses GA-BP neural network model for the prediction of the development efficiency of the human–earth system of each geographical unit from 2018 to 2025 based on the panel data of the 8 ethnic autonomous prefectures in Yunnan Province form 1995 to 2017.ResultsThe study found that from 1995 to 2017, the coordination degree and the coordinated development degree of the human–earth system in Yunnan minority areas were on the rise, but mainly manifested in the coordination of low development degree, and the average comprehensive efficiency value of input and output showed a downward trend. The prediction results for 2018–2025 showed that the human–earth system in Yunnan minority areas will be at a low efficient coordinated development level, and the coordinated development degree will show a downward trend. The development degree of natural subsystems in many ethnic minority areas showed negative growth and was negatively correlated with the development degree of economic and social subsystems. In 2025, except Honghe and Banna, the other six regions will be inefficient regions. Among these six inefficient regions, only Wenshan has input redundancy in the natural subsystem, but the phenomenon of input redundancy in the economic subsystem and insufficient output in the natural subsystem is common, indicating that industrial pollution has been controlled to a certain extent in most regions, but the economic investment mode and resource recycling efficiency need to be optimized and improved.DiscussionOur results could enrich the content of human–earth system characteristics in typical regions, and also provide theoretical support for regional coordinated development in China.
Integrative multi-omics analyses unravel the immunological implication and prognostic significance of CXCL12 in breast cancer
Authors: Gao Zhi-Jie , Fang Zhou , Yuan Jing-Ping , Sun Sheng-Rong , Li Bei 
Published: July 2023

BackgroundCXCL12 is a vital factor in physiological and pathological processes, by inducing migration of multiple cells. We aimed to comprehensively detect the role of CXCL12 in breast cancer, and explore novel CXCL12-related biomarkers through integrative multi-omics analyses to build a powerful prognostic model for breast cancer patients.MethodsImmunohistochemistry analysis of the tissue microarray was performed to evaluate the correlation between CXCL12 expression levels and breast cancer patient outcomes. Combined single-nucleus and spatial transcriptomics data was used to uncover the expression distribution of CXCL12 in breast cancer microenvironment. CXCL12-related genes were identified by WGCNA analysis. Univariate Cox and LASSO regression analyses were then conducted to screen prognostic genes from above CXCL12-related genes, followed by the construction of the CXCL12-related prognostic signature, identification of risk groups, and external validation of the prognostic signature. Analyses of biological function, mutation landscape, immune checkpoint genes and immune cells, were performed to further reveal the differences between high/low-risk groups. Paired single-cell RNA-seq and bulk RNA-seq were analyzed to further disclose the association between the risk score and the complex tumor immune microenvironment. To screen potential therapeutic agents for breast cancer patients, analyses of gene-drug correlation and sensitivity to immunotherapy were conducted.ResultsHigh expression of CXCL12 was linked with a prolonged survival in breast cancer. A total of 402 genes were identified by WGCNA analysis and 11 genes, covering VAT1L, TMEM92, SDC1, RORB, PCSK9, NRN1, NACAD, JPH3, GJA1, BMP8B and ADAMTS2, were screened as the candidate prognostic genes. Next, the prognostic signature was built and validated using these genes to predict the outcomes of breast cancers. The high-risk group patients exhibited significantly inferior prognoses. The combination of the risk score and tumor mutational burden (TMB) had remarkably improved performance in predicting patient outcomes. Besides, high-risk group patients showed higher infiltration of M2-like macrophages. Finally, several potential anticancer drugs were identified. The high-risk group patients were more sensitive to immunotherapy but resistant to docetaxel.ConclusionsCXCL12 has important immunological implication and prognostic significance in breast cancer. The CXCL12-related prognostic model could well predict the prognosis and treatment response of breast cancers.
Case report: Recurrent nocturnal awakenings in cluster headache: a different type of ghost attack
Authors: Giuliani Giada , Gorgoni Maurizio , Altieri Marta , Di Piero Vittorio 
Published: July 2023

IntroductionCluster headache (CH) is a trigeminal autonomic cephalalgia characterized by attacks of severe unilateral pain associated with ipsilateral autonomic symptoms. Cluster headache attacks exhibit nocturnal predilection, and sleep disorders could be the first manifestation of an incipient cluster period. Sleep alterations in cluster headache patients may reflect the pivotal role of the hypothalamus, which is crucially involved in the pathophysiology of this primary headache. We describe the case of a patient affected by episodic cluster headache who experienced a sleep disorder after starting therapy with verapamil.Case presentationA 47-year-old man was affected by episodic cluster headache, characterized by attacks of excruciating pain in the left orbital and temporal regions, associated with prominent ipsilateral vegetative symptoms. Headaches occurred during the night, with one or two nocturnal attacks appearing at 11.30–12 p.m. and 4–4.30 a.m. Preventive treatment with verapamil was started, with immediate pain relief. Later, he experienced consecutive nocturnal awakenings for a couple of weeks, always at the same time, without any pain or autonomic symptoms. He was not agitated and did not need to get out of bed; after the awakenings, he reported sleep disturbances with vivid dreams.Discussion and conclusionThis case represents the first description of recurrent cyclic nocturnal awakenings, without pain and autonomic symptoms, in a patient with episodic cluster headache during the active phase of a cluster bout. Nocturnal awakenings, started after the introduction of effective preventive therapy, might be an unusual form of “ghost attacks.” After the beginning of prophylactic therapy, patients often describe mild pain or localized pressure in the same localization of CH attack. Similarly, the appearance of sleep disturbances, without any pain or vegetative symptoms, should be regarded as a warning sign of a still active cluster bout. Since these manifestations may influence therapeutic management, they should be carefully investigated.
Deep intracochlear injection of triamcinolone-acetonide with an inner ear catheter in patients with residual hearing
Authors: Prenzler Nils K. , Salcher Rolf , Lenarz Thomas , Gaertner Lutz , Lesinski-Schiedat Anke , Warnecke Athanasia 
Published: July 2023

IntroductionIn a previous study, an inner ear catheter was used to deliver low- and high-dose steroids into the cochlea prior to cochlear implant electrode insertion. With this approach, more apical regions of the cochlea could be reached and a reduction of electrode impedances in the short term was achieved in cochlear implant recipients. Whether intracochlear application of drugs via the catheter is a safe method also for patients with residual hearing has not been investigated hitherto. The aim of the present study was therefore to investigate the effect of intracochlear triamcinolone application in cochlear implant recipients with residual hearing.Patients and methodsPatients with residual hearing were administered triamcinolone-acetonide (4 mg/ml; n = 10) via an inner ear catheter just prior to insertion of a MED-EL FLEX28 electrode. Impedances were measured at defined time points (intra-operatively, post-operatively and at first fitting) and retrospectively compared with a control group (no steroid application) and low- and high-dose group. Hearing thresholds were measured preoperatively, 3 days after surgery and at first fitting by pure tone audiometry. Pre- to postoperative hearing loss was determined at first fitting and compared to results from a previous study.ResultsThe median hearing loss after implantation (125–1,500 Hz) was 20.6 dB. Four patients (40%) showed a median hearing loss of less than 15 dB, three patients (30%) between 15 and 30 dB and three patients (30%) more than 30 dB. The median hearing loss was similar to the results obtained from our previous study showing a median hearing loss of 24 dB when using FLEX28 electrode arrays.ConclusionNo difference in residual hearing loss was found when comparing application of triamcinolone-acetonide using an inner ear catheter prior to the insertion of a FLEX28 electrode array to the use of the FLEX28 electrode array without the catheter. Thus, we conclude that application of drugs to the cochlea with an inner ear catheter could be a feasible approach in patients with residual hearing.
sPinal cOrd neUromodulatioN to treat Cerebral palsy in pEdiatrics: POUNCE Multisite Randomized Clinical Trial
Authors: Girshin Kristin , Sachdeva Rahul , Cohn Richard , Gad Parag , Krassioukov Andrei V. , Edgerton V. Reggie 
Published: July 2023

IntroductionCerebral palsy (CP) affects up to 4 children in 1,000 live births, making it the most common motor disorder in children. It impairs the child’s ability to move voluntarily and maintain balance and posture, and results in a wide range of other functional disorders during early development impairments in various sensory modalities, e.g., vision, hearing ability and proprioception. Current standard of care therapy focuses on symptom management and does not mitigate the progression of many of these underlying neurological impairments. The goal of this trial is to conduct a prospective multicenter, double-blinded, sham-controlled, crossover, randomized control trial to demonstrate the safety and efficacy of noninvasive spinal cord neuromodulation (SCiP™, SpineX Inc.) in conjunction with activity-based neurorehabilitation therapy (ABNT) to improve voluntary sensorimotor function in children with cerebral palsy.Methods and analysisSixty participants (aged 2–13 years) diagnosed with CP classified as Gross Motor Function Classification Scale Levels I-V will be recruited and divided equally into two groups (G1 and G2). Both groups will receive identical ABNT 2 days/wk. G1 will initially receive sham stimulation, whereas G2 will receive therapeutic SCiP™ therapy for 8 weeks. After 8 weeks, G1 will cross over and receive therapeutic SCiP™ therapy for 8 weeks, whereas G2 will continue to receive SCiP™ therapy for another 8 weeks, for a total of 16 weeks. Primary and secondary outcome measures will include Gross Motor Function Measure-88 and Modified Ashworth Scale, respectively. Frequency and severity of adverse events will be established by safety analyses.Ethics and disseminationThe trial is registered on clinicaltrials.gov (NCT05720208). The results from this trial will be reported on clinicaltrials.gov, published in peer-reviewed journals and presented at scientific and clinical conferences.