bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of self-reporting, clinicians’ assessment, and tacrolimus trough levels in evaluating adherence to immunosuppressive therapy in kidney transplant recipients. A cohort study
Authors: Dorji Choki , Tobgay Tashi , Na-Bangchang Kesara 
Published: August 2023

AbstractIntroductionImmunosuppressants are drugs with narrow therapeutic indices and pharmacokinetic variation. Nonadherence to the therapy will cause over or underexposure leading to graft rejection.MethodsA cohort study was conducted on kidney transplant recipients from the nephrology clinic and therapeutic drug monitoring unit. Patients were asked to self-report their medication adherence using a self-assessment tool. Assessment by clinicians and variation of tacrolimus levels were evaluated. Tacrolimus concentration and kidney function were measured prospectively to correlate with patients’ adherence. The variation of tacrolimus levels between 20-43 % was classified as medium and above 43 % as poor adherence.ResultsAmong 58 participants, 33 (56.7%) were females. The maximum number of years attained after transplantation was 17, with a median duration of 5 years. On the self-reporting scale, 47.0% of adherence was due to fear of graft rejection. Among nonadherences, 77.4% had their immunosuppressive therapy two hours before or after the prescribed time. Based on the clinician score, 39 (67.2%) and 42 (72.4%) cases were identified as nonadherence and having tacrolimus Ctroughlevel above 20 % respectively. The median (95% CI) serum creatine and blood urea nitrogen levels in the medium category were 1.23 mg/dL (1.2-1.4) (P = 0.009) and 28.3 mg/dL (26.4-36.4) (P = 0.021) respectively. The corresponding values for poor adherence were 2.5 mg/dL (1.6-3.5) (P = 0.03) and 43.0 mg/dL (35.5-78.0) (P = 0.01).ConclusionsThe fear of allograft rejection is linked to a better adherence rate. Nonadherent transplant recipients required close observation and frequent monitoring of immunosuppressant levels for graft survival.
Comparative Genomic and Transcriptomic Analysis of Avirulent and Virulent Strains ofFusarium oxysporumf. sp.carthami: Insights into Pathogenesis and Virulence Determinants in Safflower Infections
Authors: Aabha  , Laxmi Vijay , Singh Babita , Mehta Samriddhi , Gupta Ashish Kumar , Srivastava Alok , Sawant Samir , Katiyar-Agarwal Surekha , Paritosh Kumar , Agarwal Manu 
Published: August 2023

AbstractVascular wilt disease incited byFusarium oxysporumf. sp.carthami(Foc) in Safflower poses a significant threat to its production in India. A comprehensive understanding of the molecular underpinning of compatible and incompatible interaction is of extreme economic importance. In the present study, the genome of a virulent (IARI-5175) and a avirulent (F-00845) Foc strain were sequenced and assembled using data generated from Illumina in combination with Nanopore technologies and HiC. Foc genomes were assembled into 88 and 23 scaffolds with an estimated total size of 46 Mb and 42 Mb respectively for IARI-5175 and F-00845 strains. Reference based mapping of Foc genome withF. oxysporumf. sp.lycopersici(Fol) resulted in chromosomal level ordering of genome and simultaneous identification of accessory genome. Additionally, two lineage specific chromosomes were also identified for virulent Foc strain IARI-5175. Genomic comparisons were made on the basis of effectors, CAZymes, secondary metabolites and mycotoxins to understand the global view pathogenicity in Foc. Moreover, the transcriptome of Foc during compatible and incompatible interaction was sequenced and analyzed leading to the identification of differentially regulated genes. Taken together our study laid a solid foundation to explore novel effector genes that play a crucial role in the establishment of disease and can further be used as targets to devise new strategies to curb wilt disease in safflower.
Epithelial Ca2+waves triggered by enteric neurons heal the gut
Authors: Petsakou Afroditi , Liu Yifang , Liu Ying , Comjean Aram , Hu Yanhui , Perrimon Norbert 
Published: August 2023

SummaryA fundamental and unresolved question in regenerative biology is how tissues return to homeostasis after injury. Answering this question is essential for understanding the etiology of chronic disorders such as inflammatory bowel diseases and cancer. We used theDrosophilamidgut to investigate this question and discovered that during regeneration a subpopulation of cholinergic enteric neurons triggers Ca2+currents among enterocytes to promote return of the epithelium to homeostasis. Specifically, we found that down-regulation of the cholinergic enzyme Acetylcholinesterase in the epithelium enables acetylcholine from defined enteric neurons, referred as ARCENs, to activate nicotinic receptors in enterocytes found near ARCEN- innervations. This activation triggers high Ca2+influx that spreads in the epithelium through Inx2/Inx7 gap junctions promoting enterocyte maturation followed by reduction of proliferation and inflammation. Disrupting this process causes chronic injury consisting of ion imbalance, Yki activation and increase of inflammatory cytokines together with hyperplasia, reminiscent of inflammatory bowel diseases. Altogether, we found that during gut regeneration the conserved cholinergic pathway facilitates epithelial Ca2+waves that heal the intestinal epithelium. Our findings demonstrate nerve- and bioelectric-dependent intestinal regeneration which advance the current understanding of how a tissue returns to its homeostatic state after injury and could ultimately help existing therapeutics.
Simultaneous targeting of AMPK and mTOR is a novel therapeutic strategy against prostate cancer
Authors: Zhao Gangyin , Forn-Cuní Gabriel , Scheers Marvin , Lindenbergh Pier Pieterszoon , Yin Jie , van Loosen Quint , Passarini Leonardo , Chen Lanpeng , Snaar-Jagalska B. Ewa 
Published: August 2023

AbstractMetastatic colonization by circulating cancer cells is a highly inefficient process. To colonize distant organs, disseminating cancer cells must overcome many obstacles in foreign microenvironments, and only a small fraction of them survives this process. How these disseminating cancer cells cope with stress and initiate metastatic process is not fully understood. In this study, we report that the metastatic onset of prostate cancer cells is associated with the dynamic conversion of metabolism signaling pathways governed by the energy sensors AMPK and mTOR. While in circulation in blood flow, the disseminating cancer cells display decreased mTOR and increased AMPK activities that protect them from stress-induced death. However, after metastatic onset, the mTOR-AMPK activities are reversed, enabling mTOR-dependent tumor growth. Suppression of this dynamic conversion by co-targeting of AMPK and mTOR signaling significantly suppresses prostate cancer cell and tumor organoid growthin vitroand experimental metastasisin vivo, suggesting that this can be a therapeutic approach against metastasizing prostate cancer.
(De)composing sociality: disentangling individual-specific from dyad-specific propensities to interact
Authors: Neumann Christof , Fischer Julia 
Published: August 2023

AbstractIn socially living animals, relationships between group members are typically highly differentiated. Some dyads maintain strong and long-lasting relationships, while others are only connected by weak ties. There is growing evidence that the number and strength of social bonds are related to reproductive success and survival. Yet, few of these analyses have considered that frequent or prolonged affiliative interactions between two individuals are driven by two different processes: namely, the overall gregariousness of the individuals involved and their dyadic affinity, i.e., the preference the members of the dyad have to inter-act specifically with one another. Crucially, these two axes of sociality cannot be observed directly, although distinguishing them is essential for many research questions, for example, when estimating kin bias or when studying the link between sociality and fitness.We present a principled statistical framework to estimate the two underlying sociality axes using dyadic interaction data. We provide the R package bamoso, which builds on Stan code to implement models based on the proposed framework and allows visual and numerical evaluation of the estimated sociality axes.We demonstrate the application and some of the critical advantages of our proposed modeling framework with simulated and empirical data: (1) the possibility of checking model fit against observed data, (2) the assessment of uncertainty in the estimated sociality parameters, and (3) the possibility to extend it to more complex models that use interaction data to estimate the relationship between individual-level sociality and individual-level outcomes in a unified model.Our model will help to understand how and why individuals interact with each other and will help address questions about the relationship between variation in sociality and other features of interest, both within and across species.
Palaeoproteomic investigation of an ancient human skeleton with abnormal deposition of dental calculus
Authors: Uchida-Fukuhara Yoko , Shimamura Shigeru , Sawafuji Rikai , Nishiuchi Takumi , Yoneda Minoru , Ishida Hajime , Matsumura Hirofumi , Tsutaya Takumi 
Published: August 2023

AbstractDetailed investigation of extremely severe pathological conditions in ancient human skeletons is important as it could shed light on the breadth of potential interactions between humans and disease etiologies in the past. Here, we applied palaeoproteomics to investigate an ancient human skeletal individual with severe oral pathology, focusing our research on bacterial pathogenic factors and host defense response. This female skeleton, from the Okhotsk period (i.e., 5th–13th century) of Northern Japan, poses relevant amounts of abnormal dental calculus deposition and exhibits oral dysfunction due to severe periodontal disease. A shotgun mass-spectrometry analysis identified 81 human proteins and 15 bacterial proteins from the calculus of the subject. We identified two pathogenic or bioinvasive proteins originating from two of the three “red complex” bacteria, the core species associated with severe periodontal disease in modern humans, as well as two additional bioinvasive proteins of periodontal-associated bacteria. Moreover, we discovered defense response system-associated human proteins, although their proportion was mostly similar to those reported in ancient and modern human individuals with lower calculus deposition. These results suggest that the bacterial etiology was similar and the host defense response was not necessarily more intense in ancient individuals with significant amounts of abnormal dental calculus deposition.
HDAC6/aggresome processing pathway importance for inflammasome formation is context dependent
Authors: Wang Longlong , Unterreiner Adeline , Kapetanovic Ronan , Aslani Selma , Xiong Yuan , Donovan Katherine A. , Farady Christopher J. , Fischer Eric S. , Bornancin Frédéric , Matthias Patrick 
Published: August 2023

AbstractThe inflammasome is a large multiprotein complex that assembles in the cell cytoplasm in response to stress or pathogenic infection. Its primary function is to defend the cell and promote the secretion of pro-inflammatory cytokines, including IL-1β and IL-18. It was shown that in immortalized bone marrow derived macrophages (iBMDMs) inflammasome assembly is dependent on the deacetylase HDAC6 and the aggresome processing pathway (APP), a cellular pathway involved in the disposal of misfolded proteins. Here we used primary BMDMs from mice in which HDAC6 is ablated or impaired and found that inflammasome activation was largely normal. We also used human peripheral blood mononuclear cells and monocytes cell lines expressing a synthetic protein blocking HDAC6-ubiquitin interaction and impairing the APP and found that inflammasome activation was moderately affected. Finally, we used a novel HDAC6 degrader and showed that inflammasome activation was partially impaired in human macrophage cell lines with depleted HDAC6. Our results therefore show that HDAC6 importance in inflammasome activation is context dependent.
An anciently diverged family of RNA binding proteins maintain correct splicing of a class of ultra-long exons through cryptic splice site repression
Authors: Siachisumo Chileleko , Luzzi Sara , Aldalaqan Saad , Hysenaj Gerald , Dalgliesh Caroline , Cheung Kathleen , Gazzara Matthew R , Yonchev Ivaylo D , James Katherine , Kheirollahi Chadegani Mahsa , Ehrmann Ingrid , Smith Graham R , Cockell Simon J , Munkley Jennifer , Wilson Stuart A , Barash Yoseph , Elliott David J 
Published: August 2023

AbstractWe previously showed that the germ cell specific nuclear protein RBMXL2 represses cryptic splicing patterns during meiosis and is required for male fertility. RBMXL2 evolved from the X-linked RBMX gene, which is silenced during meiosis due to sex chromosome inactivation. It has been unknown whether RBMXL2 provides a direct replacement for RBMX in meiosis, or whether RBMXL2 evolved to deal with the transcriptionally permissive environment of meiosis. Here we find that RBMX primarily operates as a splicing repressor in somatic cells, and specifically regulates a distinct class of exons that exceed the median human exon size. RBMX protein-RNA interactions are enriched within ultra-long exons, particularly within genes involved in genome stability, and repress the selection of cryptic splice sites that would compromise gene function. These similarities in overall function suggested that RBMXL2 might replace the function of RBMX during meiosis. To test this prediction we carried out inducible expression of RBMXL2 and the more distantly related RBMY protein in somatic cells, finding each could rescue aberrant patterns of RNA processing caused by RBMX depletion. The C-terminal disordered domain of RBMXL2 is sufficient to rescue proper splicing control after RBMX depletion. Our data indicate that RBMX and RBMXL2 have parallel roles in somatic tissues and the germline that must have been conserved for at least 200 million years of mammalian evolution. We propose RBMX family proteins are particularly important for the splicing inclusion of some ultra-long exons with increased intrinsic susceptibility to cryptic splice site selection.
Tazobactam selects for multidrug resistance
Authors: Holden Emma R. , Yasir Muhammad , Turner A. Keith , Charles Ian G. , Webber Mark A. 
Published: August 2023

AbstractPiperacillin-Tazobactam is a β-lactam/β-lactamase inhibitor combination which is amongst the most prescribed antimicrobials in hospital medicine. Piperacillin is inactivated by commonly carried resistance enzymes, but tazobactam inhibits these allowing successful treatment. The effect of piperacillin on Gram-negative bacteria has been widely studied, but less attention has been paid to the effects of tazobactam. We used a massive transposon mutagenesis approach (TraDIS-Xpress) to determine the genes inEscherichia colithat affect survival when exposed to piperacillin and tazobactam, separately and together. We found significant differences in the selective pressure of the two drugs: a striking finding was that multiple efflux pump families and regulators were essential for survival in the presence of tazobactam, but only one efflux system was beneficial for piperacillin. Additionally, we identified the shikimate kinase AroK as a potential target for tazobactam. This method also found that genes involved in DNA replication and repair reducedE. colisusceptibility to a combination of piperacillin and tazobactam, not seen from either drug treatment alone. Treatment ofE. coliandKlebsiella pneumoniaewith piperacillin and/or tazobactam selected for mutants with reduced susceptibility, and SNP analyses supported the TraDIS-Xpressfindings that tazobactam selects for changes in membrane permeability and maintenance associated with multidrug-resistance. Increased efflux activity is an important foundation of multidrug resistance in human pathogens, therefore the finding that tazobactam can select for this is concerning. These findings could have consequences for antibiotic prescription and should inform the development of future β-lactamase inhibitors to reduce the global increase in multidrug-resistant infections.
The theory of sustainability values and travel behavior
Authors: Sirakaya-Turk Ercan , Oshriyeh Omid , Iskender Ali , Ramkissoon Haywantee , Mercado Haylee Uecker 
Published: August 2023

Purpose
This paper reports the results of research that examines the interrelationships between efficacy of sustainability values (SV) and pro-sustainable behaviors of potential tourists. A partially mediated model is postulated and tested to help explain additional error variance in predicting consumers’ destination choice decisions in tourism, hence voiding a critical research gap. Coined as the “environmentally intellectualist behavior,” a new mediator variable is tested to explain additional error variance in human-value models.

Design/methodology/approach
The study is based on data collected from two representative samples of potential tourists from the USA and Canada. Data analyses include exploratory and confirmatory factor analyses that were used to examine the underlying domain structures of SV, followed by a predictive model using structural equation modeling.

Findings
The study findings suggest that values are salient factors that underlie pro-sustainable tourism and travel behavior. Moreover, the results confirm the existence of a higher-order sustainability construct. The study contributes original insights to the field by demonstrating that there are direct and indirect positive relationships between SV, environmental behaviors and decisions of consumers who take a pro-sustainable stance when traveling.

Originality/value
By modeling values as antecedents to attitudes and testing interrelationships between SV and the mediator variables coined as the environmentally intellectual behavior, the authors developed and tested a predictive model to explain destination- and product choice decisions. The model tested herein advances the value theory in two fundamental ways: first, this study demonstrates that SV can be modeled as higher-order factors. Second, values are antecedents to attitude and other variables, therefore must be included in consumer behavior models. Finally, the culture or origin of tourists matters when examining the impact of values on tourists’ choice decisions. Political actions and environmental attitudes can be modeled as mediators to explain additional error variance.
The effect of teacher-student collaboration on academic innovation in universities: an exploration from the perspective of collaborative process
Authors: Pan Wenhui , Liu Zhenxing 
Published: August 2023

Purpose
This paper aims to explore the effect of teacher–student collaboration on academic innovation in universities in different stages of collaboration.

Design/methodology/approach
Based on collaboration life cycle, this paper divided teacher–student collaboration into initial, growth and mature stages to explore how teacher–student collaboration affects academic innovation.

Findings
Collecting data from National Science Foundation of China, the empirical analysis found that collaboration increases the publication of local (Chinese) papers at all stages. However, teacher–student collaboration did not significantly improve the publication of international (English) papers in the initial stage. In the growth stage, teacher–student collaboration has a U-shaped effect on publishing English papers, while its relationship is positive in the mature stage.

Practical implications
The results offer suggestions for teachers and students to choose suitable partners and also provide some implications for improving academic innovation.

Originality/value
This paper constructed a model in which the effect of teacher–student collaboration on academic innovation in universities was established.
Esports fan identity toward sponsor–sponsee relationship: an understanding of the role-based identity
Authors: Calapez André , Ribeiro Tiago , Almeida Victor , Pedragosa Vera 
Published: August 2023

PurposeDespite to useful relevance to better understand how group-level identity develops, few studies have explored the identity theory in the esports field and, in particular, considering the impact of a fan's role identity. The current study aims to explore esports fan role-identity vis-à-vis the relationship with the sponsor and the sponsee so as to understand the effects on their behavioral intentions.Design/methodology/approachUsing a sample of 356 esports fans who attended the 2021 FPF eFootball Open Challenge, a Confirmatory Factor Analysis (CFA) analyzed the psychometric properties of the constructs and a subsequent Structural Equation Modeling (SEM) examined the effects of fan identity on two types of behavioral intentions and sponsor–sponsee relationship.FindingsResults indicate that fans who highly identify with esports have the highest attachment to the event and tend toward having a positive word-of-mouth intention. Esports fans who have a higher brand identification reported a positive attitude toward the event's sponsor brand and tend to purchase its products. Moreover, the study findings also provide evidence of the bidirectional interaction between the way in which fans attach with the esports event and its sponsor brand, leading to greater reciprocity in their identity formation.Originality/valueThis study helps to understand how the fan identity process can enhance its fate and develop mutually, building role overlapping identity in the esports sponsor–sponsee relationship. Complementarily, it supports of how the marketeers and managers must analyze the importance of being a fan to the individual in order to understand how its self-identity can shape the future behavior.
Effect of droplet size on the film formation of O/W emulsion
Authors: Liu Yuan , Dong Chang , Wang Xianzhang , Sang Xiao , Ma Liran , Xu Xuefeng , Tian Yu 
Published: August 2023

Purpose
The purpose of this study is to reveal the underlying mechanism in film formation of oil-in-water (O/W) emulsion.

Design/methodology/approach
This study focuses on the film forming characteristics of O/W emulsion between the surface of a steel ball and a glass disc coated with chromium. The lubricant film thicknesses of O/W emulsion with various mechanical stirring strength were discussed, which were observed by technique of relative optical interference intensity.

Findings
The authors directly observed the oil pool in the contact area, finding the size of oil pool was closely related to the film-forming ability of emulsion. Enrichment phenomenon occurs in oil pool, which was caused by phase inversion. Further investigations revealed that the emulsion is stable with strong stirring strength, resulting in a smaller oil pool size and worse film forming ability.

Originality/value
With the wide usage of O/W emulsion in both biological and industrial systems, the ability of emulsion film formation is considered as an important factor to evaluate the lubrication effectiveness.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-12-2022-0354/