bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a risk prediction model for gestational diabetes mellitus in women of advanced maternal age during the first trimester
Authors: Tang Yao , Liu Zhenzhen , Li Li , Liu Haiyan , Li Xiaotian , Gu Weirong 
Published: January 2025

AbstractWith the global rise in advanced maternal age (AMA) pregnancies, the risk of gestational diabetes mellitus (GDM) increases. However, few GDM prediction models are tailored for AMA women. This study aims to develop a practical risk prediction model for GDM in AMA women. Data were obtained from a prospective observational cohort of AMA pregnant women from the Obstetrics and Gynecology Hospital in Shanghai, China. Singleton pregnancies with complete OGTT results at 24–28 weeks were selected and divided into training (70%) and validation (30%) sets. First‐trimester predictors, including demographic, metabolic parameters, and clinical history, were evaluated for statistical significance. A multivariate logistic regression model was developed, with performance evaluated using receiver operating characteristic (ROC) curves and calibration plots. Predictors were primarily incorporated as categorical variables in a nomogram to enhance model convenience. A model using continuous predictors was also tested for comparison. A total of 1904 AMA women were included, with GDM incidence rates of 18.3% (243/1333) in the training set and 19.3% (110/571) in the validation set. Significant predictors for GDM diagnosis at 24–28 weeks included maternal age, GDM history, first‐trimester fasting plasma glucose, mean arterial pressure, and triglyceride levels. The categorical model achieved an area under the ROC curve of 0.717 (95% CI: 0.682–0.753) in the training set and 0.702 (95% CI: 0.645–0.758) in the validation set. The Hosmer‐Lemeshow test indicated good calibration (p = .97 in the training set; p = .66 in the validation set). The model with category and continuous predictors exhibited similar performance. This study developed and validated a practical early risk prediction nomogram for GDM in AMA women, using commonly available clinical data. The model shows good predictive performance and is resource‐efficient, making it suitable for real‐world clinical implementation.
The function of TRIML2 on the temozolomide resistance in glioblastoma
Authors: Fu Qiang , Chen Peipei , Wang Zening , Liu Bo , Zhou Qingjiu , Anwar Ilhamjan , Wang Yongxin 
Published: January 2025

Background:
Acquired resistance to temozolomide is a major challenge for the effective treatment of glioblastoma (GBM). TRIML2, a member of the TRIM family, plays an important role in cancer genesis, progression, and treatment resistance. However, its mechanism of action in GBM resistance to temozolomide remains unclear.

Methods:
RNA bulk sequencing data from temozolomide-resistant U87 cells and wild-type U87 cells were downloaded from the NCBI public database (GEO: GSE193957) and analyzed. A temozolomide-resistant cell line (U87-TR) was induced with temozolomide, and the expression of TRIML2 in temozolomide-resistant and wild-type cell lines (U87-WT) was verified by cell activity assays, wound-healing assays, and western blotting. The alteration of resistance to temozolomide was assessed following the overexpression of TRIML2 in the resistant cell line by lentiviral transfection. The differences in TRIML2 expression between primary GBM and recurrent GBM after temozolomide chemotherapy were verified by immunofluorescence, immunohistochemistry, and western blotting.

Results:
The expression of TRIML2 was significantly lower in U87-TR cells than in U87-WT cells. After the TRIML2 overexpressed in U87-TR cells, their resistance to temozolomide was significantly decreased and became sensitive to temozolomide treatment. TRIML2 expression was significantly decreased in the temozolimide-resistant GBM tumors; in contrast, TRIML2 was relatively high expressed in the temozolimide-sensitive GBM tumors.

Conclusions:
TRIML2 inhibits temozolomide resistance in GBM and thus may serve as a novel therapeutic target for overcoming GBM resistance to temozolomide.
Prescription of pregabalin for prevention of acute post-mastectomy pain syndrome (APMPS): a systematic review and meta-analysis of randomized controlled trials
Authors: Ganduboina Rohit , Dutta Palak , Pawar Shubhadarshini G. , Mukherjee Indraneil 
Published: January 2025

Background:
Mastectomy is generally considered the most effective treatment option for breast cancer. However, it is linked to a variety of complications that contribute to an elevated morbidity. Acute Post Mastectomy Pain Syndrome (APMPS) is 2 months or more of neuropathic pain after mastectomy. Pregabalin, a powerful central nervous system inhibitor is commonly prescribed for the relief of neuropathic pain. This study evaluates its efficacy in curing neuropathic pain and enhancing mastectomy pain management.

Methods:
We conducted a comprehensive literature search comparing pregabalin with placebo using relevant syntaxes. Postoperative acute pain scores, 24-hour morphine use, dizziness, and sedation were evaluated. A P-value of less than 0.05 was considered statistically significant.

Results:
Ten studies with a total of 719 patients were included. The pregabalin group exhibited significantly lower acute pain scores compared to the placebo group (standard mean difference: −0.61; 95% confidence interval: −1.02 to −0.20; P < 0.01). No statistically significant differences were observed in 24-hour morphine consumption (standardized mean difference, −2.74; 95% CI, −6.27; −0.79, P > 0.05, I
2 = 97.6%), dizziness (RR, 1.49; 95% CI, 0.82; 2.71, P > 0.05, I
2 = 1.0%) or sedation (RR, 1.38; 95% CI, 0.11; 17.84, P > 0.05, I
2 = 0.0%) between the groups. The acute pain scores showed significant heterogeneity, with an I
2 value of 78.6%.

Discussion:
This meta-analysis indicates that pregabalin may be effective for managing APMPS. However, due to the heterogeneity and limitations of the included studies, the findings should be interpreted with caution. Future research should focus on larger sample sizes, incorporating potential moderating factors to more precisely determine pregabalin’s role in APMPS.
Quality of life and oncologic safety of axillary reverse mapping in patients with breast cancer: a systematic review and meta-analysis
Authors: Fan Yu-Chen , Li Lin , Meng Xiang-Chao 
Published: January 2025

Background:
The axillary reverse mapping (ARM) technique has recently been developed to prevent lymphedema by preserving upper extremity lymphatic drainage. In this article, we evaluated the quality of life and oncologic outcomes of ARM used in patients with breast cancer.

Methods:
PubMed, Embase, and other databases were searched for relevant articles published between 2013 and 2022. Randomized clinical trials were identified and included in the meta-analysis. The evaluated endpoints included lymphedema, the number of retrieved lymph nodes (LNs), the number of patients with positive LNs, and shoulder restriction. A meta-analysis was performed using RevMan 5.3 software. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to analyze dichotomous data.

Results:
Seven studies involving 1,820 patients were included for the meta-analysis, which revealed that ARM was associated with a reduced incidence of lymphedema (OR, 0.30; 95% CI, 0.16–0.58; P = 0.0003). There were no significant differences in the number of retrieved LNs, the number of patients with positive LNs, and shoulder restriction between the two groups.

Conclusion:
The ARM procedure achieved comparable success with a better quality of life and acceptable safe oncologic outcomes for patients with breast cancer. Future multicenter randomized controlled trials are needed to further evaluate the efficacy and safety of ARM.
Post-surgical natural history of MEN2A with RET C634R mutation and bilateral cervical node metastasis: a case report
Authors: Adhikari Bindira , Niraula Biraj , Lamichhane Samit , Sapkota Aashish 
Published: January 2025

Introduction and importance:
Multiple endocrine neoplasia type 2A (MEN2A) is a rare hereditary cancer syndrome marked by medullary thyroid carcinoma (MTC), pheochromocytoma, and primary hyperparathyroidism. The RET proto-oncogene mutation, particularly the C634R variant, significantly increases the risk of persistent or recurrent disease. This case underscores the importance of genetic analysis and comprehensive follow-up in managing MEN2A.

Case presentation:
We report a 36-year-old pregnant female with MEN2A, diagnosed with the RET C634R mutation. Ten years earlier, she underwent a total thyroidectomy for MTC, followed by an adrenalectomy for bilateral pheochromocytoma. Despite these interventions, her calcitonin levels never normalized, and recent imaging revealed somatostatin receptor-expressing lesions in the left paratracheal region. Genetic testing confirmed the RET C634R mutation, also found in her 7-year-old daughter, indicating familial transmission.

Clinical discussion:
The RET C634R mutation is associated with a high risk of MTC recurrence and poor prognosis, emphasizing the need for vigilant monitoring. This case highlights the challenges in managing MEN2A, particularly in the presence of persistent lymph node involvement. It also stresses the importance of genetic counseling for at-risk family members.

Conclusion:
This case emphasizes the critical role of genetic testing, continuous monitoring, and a multidisciplinary approach in MEN2A management. The identification of the RET C634R mutation in both the patient and her daughter highlights the need for early intervention and personalized treatment strategies to improve outcomes.
The 100 most-cited papers on esthesioneuroblastoma: a bibliometric analysis
Authors: Badary Amr , Alrubaye Sura N. , Andrew Awuah Wireko , Almealawy Yasser F. , Al-Barbarawi Mohammad , Moustafa Wahab , Jawed Hira , Chaurasia Bipin , Atallah Oday 
Published: January 2025

Objective:
Esthesioneuroblastoma (ENB) or olfactory neuroblastoma, an infrequent neuroectodermal tumor primarily affecting the nasal cavity, manifests sporadically or in various clinical contexts. Bibliometric analyses have become a technique for evaluating the influence of papers in present-day clinical practice.

Methods:
This article conducted a meticulous analysis of the top 100 cited articles concerning ENB, using Scopus and employing keywords (“Esthesioneuroblastoma” and “Olfactory Neuroblastoma”). Articles focusing on ENB were systematically evaluated. Data extraction encompassed comprehensive analyses of articles, authors, citation per year, total citations, and journals, categorizing publications into distinct domains: clinical features, histopathology, article types, or radiological aspects.

Results:
The search yielded a substantial pool of 400 articles, from which the top 100 amassed a total of 10 900 citations, averaging 109 citations per article. These influential publications spanned a wide array of 47 journals, published between 1960 and 2019, involving contributions from diverse institutions across 14 countries. Notably, a significant proportion (45%) of contributions was made before the 2000, with the most prolific decade being 2000–2010, contributing 38 publications.

Conclusion:
This bibliometric study summarizes the most-cited articles on esthesioneuroblastoma, providing light on the field and its seminal works that have shaped both present-day clinical treatment and the trajectory of future research.
Successful management of secondary aorto-enteric fistula: a case report from Nepal
Authors: Chaulagain Uttam , Pokhrel Kailash Mani , Khanal Kapil , Gaurav Bishal , Kansakar Prasan Bir Singh , Shrestha Uttam Krishna 
Published: January 2025

Introduction:
Aorto-enteric fistulas (AEFs) are rare but serious causes of gastrointestinal bleeding, classified into primary and secondary types. This report describes the first documented case of a secondary AEF from Nepal, emphasizing the need for prompt diagnosis and surgical intervention.

Case presentation:
A 36-year-old male presented with black tarry stools and abdominal pain, 7 years after aorto-right common femoral artery and aorto-left common iliac artery bypass surgery for common iliac artery occlusive disease. He had a history of recurrent graft infections. Examination revealed hypotension, tachycardia, and melena. Upper gastrointestinal endoscopy showed the aortic graft in the duodenum, with CECT confirming an aortic graft inside the bowel lumen, leading to a diagnosis of secondary aorto-enteric fistulas (SAEF).

Discussion:
SAEFs often arise from graft infection and mechanical erosion by the pulsatile graft. Patients typically present with gastrointestinal bleeding, and early diagnosis is crucial, as untreated SAEFs have a mortality rate approaching 100%. The combined use of upper gastrointestinal endoscopy and CECT is essential for identifying and guiding the surgical intervention of SAEFs. This case underscores the importance of early diagnosis and early surgical intervention for successful management of SAEF.

Conclusion:
This case highlights the importance of suspecting secondary AEFs in patients with aortic graft presenting with gastrointestinal bleeding. Early diagnosis and timely surgical intervention are essential for improving patient outcomes.
Primary supratentorial extradural hydatid cyst of the brain
Authors: Fazairov Ravshan , Almureisi Hussein , Islomov Jamshid , Okon Inibehe Ime , Chaurasia Bipin 
Published: January 2025

Introduction and importance:
Hydatidosis is a parasitic infestation caused by the larva of the cestode Echinococcus granulosus. It is a zoonotic disease, with humans serving as accidental intermediate hosts, often affecting pediatric populations. The most common clinical presentations involve the liver and lungs, with cerebral hydatidosis being extremely rare. Imaging typically reveals single cystic lesions, usually unilocular and intraaxial. Extradural hydatid cysts, whether primary or secondary, are exceedingly rare. This report discusses a pediatric primary osteolytic extradural hydatid cyst of brain.

Case presentation:
A 10-year-old Yemeni male from a rural area presented with progressive headache, left hemiparesis, and right parietal soft swelling. Imaging, surgical, and histopathological findings are reviewed, highlighting the rarity of this condition in the pediatric population.

Clinical discussion:
Extradural hydatid cysts, whether primary or secondary are very rare and even very rare in children.

Conclusion:
In most cases, cerebral hydatid cysts are primary. Intracranial extradural localization of a hydatid cyst is very rare. The clinical features of cerebral hydatid cysts are closely related to their location. Because of the very slow increase in size and volume of the cyst, the intensification of symptoms may also be very slow. Timely diagnosis and proper treatments leads to cure of the disease.
Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling
Authors: Lenoir Solène , Thouvenot Elliot , Gropplero Giacomo , Dec Léonie , Loew Damarys , Théry Clotilde , Perez Jose E , Wilhelm Claire 
Published: January 2025

ABSTRACTThis study presents a novel method and device for the hydrodynamic production of extracellular vesicles (EVs) derived from biomimetic multicellular 3D spheroids, enabling high-throughput particle release that is 10 to 20 times higher than in non-stimulated conditions. The device facilitates the formation of spheroids from human mesenchymal stem cells (hMSCs), offering an all-in-one approach for both spheroid generation and EV release. Production times are reduced to just a few hours, with yield further increased by alternating periods of high hydrodynamic flow and spheroid recovery in a sequential production approach. Using this system, we explored the impact of hydrodynamic and starvation conditions on the protein cargo of EVs, identifying distinct protein markers through proteomics. Specifically, hydrodynamic stimulation enriched EVs in plasma membrane-derived and mitochondrial proteins, revealing divergent biogenesis pathways. Importantly, the produced EVs exhibited therapeutic properties, with demonstrated effects in wound healing, angiogenesis, and anti-inflammatory responses, some showing enhanced efficacy under hydrodynamic stimulation.
Study protocol: A national cross-sectional study on psychology and behaviour investigation of Chinese residents in 2024, PBICR
Authors: Fan Siyuan , Li Yimiao , Dong Fangjie , Chen Wenwen , Han Qin , Duan Tingyu , Zhang Jinguang , Sun Qiang , Bao Yanping , Li Yujia , Zang Shuang , Zhou Xiaoming , Jiang Feng , Ming Wai kit , Sun Xinying , Wu Yibo 
Published: January 2025

AbstractIntroductionThis study protocol specifies the primary research line and theoretical framework of the 2024 Survey of the Psychology and Behavior of the Chinese Population. It aims to establish a consistent database of Chinese residents’ psychological and behavioral surveys through multi center and large sample cross sectional surveys to provide robust data support for developing research in related fields. It will track the public’s physical and psychological health more comprehensively and systematically.MethodsThe study was conducted from June 23, 2024 to September 29, 2024, using stratified and quota sampling methods. A total of 150 cities across 800 communities/villages were surveyed, selected from China (Despite extensive coordination, we have been unable to contact our counterparts in the Taiwan region of China to obtain relevant statistical data). The questionnaires were distributed to the public one on one and face to face by trained surveyors. The questionnaires included basic information about the individual, personal health status, basic information about the family, the social environment in which the individual lives, psychological condition scales, behavioral level scales, other scales, and attitudes towards topical social issues. Supervisors conducted quality control during the distribution process and returned questionnaires, logically checked and cleaned for data analysis.DiscussionData collection has been finished, and scientific outputs based on this data will support the development of health promotion strategies in China and globally. In the aftermath of the pandemic, it will guide policymakers and healthcare organizations to improve their existing policies and services to maximize the physical and mental health of the Chinese population.
The Power of Three: Dynactin associates with three dyneins under load for greater force production
Authors: Rao Lu , Liu Xinglei , Arnold Mirjam , McKenney Richard J. , Stengel Kristy , Sidoli Simone , Berger Florian , Gennerich Arne 
Published: January 2025

AbstractCytoplasmic dynein is an essential microtubule motor protein that powers organelle transport and mitotic spindle assembly. Its activity depends on dynein-dynactin-cargo adaptor complexes, such as dynein-dynactin-BicD2 (DDB), which typically function with two dynein motors. We show that mechanical tension recruits a third dynein motor via an auxiliary BicD adaptor binding the light intermediate chain of the third dynein, stabilizing multi-dynein assemblies and enhancing force generation. Lis1 prevents dynein from transitioning into a force-limiting phi-like conformation, allowing single-dynein DDB to sustain forces up to ∼4.5 pN, whereas force generation often ends at ∼2.5 pN without Lis1. Complexes with two or three dyneins generate ∼7 pN and ∼9 pN, respectively, consistent with a staggered motor arrangement that enhances collective output. Under load, DDB primarily takes ∼8 nm steps, challenging existing dynein coordination models. These findings reveal adaptive mechanisms that enable robust intracellular transport under varying mechanical demands.
Unlocking the Secrets of NSP3: AlphaFold2-assisted Domain Determination in SARS-CoV-2 Protein
Authors: Edich Maximilian , Briggs David , Gao Yunyun , Thorn Andrea 
Published: January 2025

AbstractNon-structural protein 3 (nsp3) is crucial for the SARS-CoV-2 infection cycle. It is the largest protein of the virus, consisting of roughly 2000 residues, and a major drug target. However, due to its size, disordered regions, and transmembrane domains, the atomic structure of the whole protein has not yet been established. Only 10 out of its 16 domains were individually determined in experiments.Here, we demonstrate how structural bioinformatics, AI-based fold prediction, and traditional experiments complement each other and can shed light on the makeup of this important protein, both in SARS-CoV-2 and related viruses. Our method can be generalized for other multi-domain proteins, so we describe it in detail.Our prediction-based approach reveals a previously undescribed folded domain, which we could confirm experimentally. Our research also suggests a potential function of the nidovirus-wide conserved domain Y1: This domain may be involved in the assembly of nsp3, nsp4, and nsp6 into the hexameric pore, which was discovered by electron tomography and exports RNA into the cytosol. The Y1-hexamer, however, could not be expressed and purified on its own. We also provide a revised domain segmentation and nomenclature of nsp3 domains based on a compilation of previous research and our own findings.
Optical recordings of unitary synaptic connections reveal high and random local connectivity between CA3 pyramidal cells
Authors: Layous Robert , Tamás Bálint , Mike Arpad , Sipos Eszter , Arszovszki Antónia , Brunner János , Szatai Ádám , Yaseen Farah , Andrási Tibor , Szabadics János 
Published: January 2025

AbstractThe hippocampal CA3 region is thought to play crucial roles in episodic memory functions because of the extensive recurrent connections between CA3 pyramidal cells (CA3PCs). However, different methods provided contradicting observations about the synaptic connectivity between CA3PCs. Therefore, we estimated the connectivity rate between individual CA3PCs using a new approach that is not affected by the confounds of conventional methods. Specifically, we used voltage imaging with the Voltron sensor in acute slices from rats of both sexes to test CA3PC connections by detecting spontaneous spiking and subthreshold responses in anatomically identified neurons. We detected 164 monosynaptic excitatory connections in 3078 tested CA3PC-CA3PC pairs. This 5.3% connectivity rate was much higher than that we observed with the theoretically more sensitive patch clamp method in similar experimental conditions, but it remained below the anatomically observed number of CA3PC-CA3PC contacts. We verified that the imaged excitatory connections were mediated by AMPA receptors. Our results also showed that the recurrent connections did not enrich into preferred connectivity motifs and followed a distribution that was consistent with random connectivity in general. Moreover, voltage imaging revealed CA3PCs with distinct firing properties and somatic locations corresponding to previously established heterogeneity and showed that specific connectivity rules create preferred information routes among these subpopulations. Finally, we showed that there is at least one condition, influencing patch clamp recordings but not voltage imaging, that affects the observable functional connections between CA3PCs. Altogether, our results obtained with a new voltage imaging approach argue for high local connectivity rates between CA3PCs.Significance StatementVoltage imaging offers new opportunities to measure synaptic connectivity between individual identified neurons. We employed the Voltron sensor to measure the monosynaptic connectivity between local CA3 pyramidal cells (CA3PCs) in acute slices from rats. Recurrent CA3PC connections are thought to play crucial roles in storing memory. However, the estimated connectivity rates between CA3PCs differ depending on the methods used. We showed that in spite of having a lower signal-to-noise ratio, voltage imaging results showed a much higher connectivity between CA3PCs than patch clamp recordings. Our results also suggest that the recurrent excitatory connections between CA3PCs are random, with preferred connectivity between and within subpopulations of CA3PCs.