bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of 0.0625%,0.075% and 0.1% ropivacaine on Epidural-Related Maternal Fever and inflammatory factors: a prospective and randomized control trial
Authors: Xiao Biru , Wang Zilu , Xiong Yicheng , Li Xinmiao , Yang Xiaodan , Li Juan , Zhang Hang , Wen Wen , Feng Sizhe , Chen Hongfei , Xiong Xiangqing 
Published: February 2023

Abstract

Backgroud: Women in labor who receive epidural analgesia are more likely to experience intrapartum fever, which is defined as Epidural-related maternal fever (ERMF). The mechanisms of ERMF remain incompletely understood. At present, there are no effective means to prevent epidural-associated fever. This study was to investigate the effects of different concentrations of ropivacaine on intrapartum fever and pyrogenic factors during epidural labor analgesia. Methods 123 full-term single first-term primiparas who were hospitalized for delivery and willing to receive patient-controlled epidural analgesia (PCEA) were selected as research objects. Analgesia was randomly provided with one of three groups: (Group A) 0.0625% ropivacaine with 1ug/ml fentanyl, (Group B) 0.075% ropivacaine with 1ug/ml fentanyl, (Group C) 0.1% ropivacaine with 1ug/ml fentanyl. The primary outcome was the tympanic temperature. The secondary outcomes included plasma inflammatory factor levels, VAS score, labor duration and neonatal Apgar score. Results At T2(T2 = four hours after analgesia), the average body temperature of Group C was higher than that of the other two groups (P < 0.05, Table 2), but there was no significant difference between Group A and Group B (P > 0.05, Table 2). At T3(T3 = eight hours after analgesia), the average temperature of Group C was higher than that of Group B and that of Group B was higher than that of Group A (P < 0.05, Table 2). At T2, plasma thermogenesis factors IL-6, TNF-α, PGE2 and RANTES in Group C were higher than those in Group A and Group B (P < 0.05), but there was no significant difference between Group A and group B (P > 0.05). At T3, the above inflammatory factors in group C were higher than those in group B (P < 0.05), and those in group B were higher than those in group A (P < 0.05). There were no significant differences in delivery time, VAS score and neonatal Apgar score among the three groups (P > 0.05). Conclusions This study suggests that lower concentration of ropivacaine results in lower tympanic temperature, which was associated with lower levels of inflammatory factors. Trial registration: This prospective and randomized control trial was registered at Chinese Clinical Trial Registry (https://www.chictr.org.cn/edit.aspx?pid=141149&htm=4, identification number: ChiCTR2100053761; principal investigator: Xiangqing Xiong; date of registration: 29/11/2021) before patient enrollment.
A nomogram for predicting prognosis in patients with acute respiratory distress syndrome (ARDS) and acute renal injury (AKI): A retrospective cohort study
Authors: Yu Xueshu , Zhang Xianwei , Xu Wen , Ruan Xiangyuan , Ye Yincai , Pan Jingye 
Published: February 2023

Abstract

Background Acute respiratory distress syndrome (ARDS) combined with acute kidney injury (AKI) remains a challenge for clinicians. Early identification of high-risk patients is essential to ensure proper management. However, the present literature does not provide such an instrument. The purpose of this study is to develop a fast and easy to manage instrument to predict the prognosis of patients with ARDS and AKI. Methods We extracted data from Medical Information Mart for Intensive Care-IV v2.0. Variable selection was based on LASSO regression. Then, we constructed a nomogram model and the performances of the model were evaluated with area under the curve (AUC), and decision curve analysis (DCA) respectively. Results We have developed a novel and practical nomogram that accurately predicted ARDS combined with AKI. The AUC of the novel model was better than that of SOFA and SPAS II (all P < .001). DCA showed that the nomogram model had a better net benefit than SOFA and SPAS II. Conclusions We had developed a novel scoring tool that accurately predicts the prognosis of ARDS combined with AKI and may help improve patient outcomes. This finding, however, needs to be confirmed by external validation as well as multi-centre prospective studies.
Impact of Altering the Method to Score During Small-sided Games on Internal and External Training Loads in Youth Soccer Players
Authors: Silva Marxno Ermesony Sabino da , Ribeiro Bruno Laerte Lopes , Ferreira Ayrton Bruno de Morais , Nakamura Fábio Yuzo , Oliveira Ricardo Santos , Mortatti Arnaldo Luis 
Published: February 2023

Abstract
INTRODUCTION: Diverse strategies to manipulate acute training load can be used in small-sided games (SSG). However, how different methods to score during SSG influence internal and external workloads in young athletes is still under debate.
OBJECTIVE: To compare external and internal training loads between SSGs played with different rules to score in young soccer players.
METHODS: In separated days, 13 young athletes (age: 14.7±0.4 years) performed three different SSGs models: ball possession (BP-SSG), score zone (SZ-SSG), and SSG with small-goals (SG-SSG). During the SSGs players worn accelerometers to obtain raw acceleration values which were later transformed in external training load (ETL) measured as PlayerLoad®. ~30-min following the SGGs internal training load (ITL) was obtained as the session-rating of perceived exertion (session-RPE).
RESULTS: The BP-SSG (369.05±66.6 a.u.) led to higher ETL compared to the SZ-SSG (326.22±50.6 a.u.), and to the SG-SSG (288.08±52.92 a.u.). Similarly, the BP-SSG (238.69±32.21 a.u.) also caused higher ITL compared to the SZ-SSG (208.57±32.17 a.u.), and to the SG-SSG (187, 38±30.4 a.u.).
CONCLUSION: In young soccer players, SSGs with the aim to maintain ball possession caused higher ITL and ETL compared to SSG with other methods to score. Coaches are encouraged to use SSG with the aim to maintain ball possession when designing high-intensity sessions, whereas for low-intensity sessions SSG with the small-goals zone can be used.
Cajanus cajan (L) Millsp. seed extract ameliorated scopolamine-induced amnesia through increase in antioxidant defense mechanisms and cholinergic neurotransmission
Authors: Olubodun-Obadun Taiwo Grace , Ishola ismail O , Akinwande Abisola Sekinat , Adeyemi Olufunmilayo Olaide 
Published: February 2023

Abstract
Decline in cholinergic function and oxidative/nitrosative stress play a central role in Alzheimer’s disease (AD). Our quantitative preliminary study revealed the presence of several neuroprotective flavonoids in Cajanus cajan seed extract. Hence, this study was designed to investigate the protective action of Cajanus cajan ethanol seed extract (CC) on learning and memory functions using scopolamine mouse model of amnesia. Adult mice were first pretreated with CC (50, 100, or 200mg/kg, p.o) or vehicle (10ml/kg, p.o) for 16 days. Scopolamine an anti-muscarinic agent (1mg/kg) was given an hour after CC pretreatment from days 3 to 16. The mice were subjected to behavioural tests (open field test (OFT), Y-maze test (YMT) or Morris water maze task (MWM). Animals were euthanized on day 16 and biochemical analysis was carried on the brain tissue to assay for biomarkers of oxidative stress and cholinergic system. Molecular docking analysis was also done to investigate the possible mechanism(s) through which CC exerts its anti-amnesic action. The results showed that the pre-administration of CC ameliorated working memory and learning deficits caused by scopolamine in YMT and MWM tests, respectively. CC improved cholinergic function via attenuation of scopolamine-induced increase in acetylcholinesterase activity in the hippocampus. Furthermore, CC attenuated scopolamine-induced oxidative stress and increased nitrite generation in the hippocampus evidenced in significant increase in glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities with a marked decrease in malondialdehyde (MDA) levels. Phytochemicals obtained from CC displayed significant interaction at the M1 muscarinic acetylcholine receptor, alpha-7 nicotinic acetylcholine receptor and amyloid beta peptide binding pockets which suggests the involvement of these target proteins in the activity of CC. Findings obtained from this study showed that CC improved working memory and learning through enhancement of cholinergic systems and reduction in amyloidogenesis.
A platform for deep learning on (meta)genomic sequences
Authors: Münch Philipp , Mreches René , To Xiao-Yin , Gündüz Hüseyin Anil , Moosbauer Julia , Klawitter Sandra , Deng Zhi-Luo , Robertson Gary , Rezaei Mina , Asgari Ehsaneddin , Franzosa Eric , Huttenhower Curtis , Bischl Bernd , McHardy Alice , Binder Martin 
Published: February 2023

Abstract
We describe deepG, a platform for the generation, interpretation, and application of deep learning models for sequence labeling, classification, and regression based on nucleotide sequences. Unlike other bioinformatic deep learning platforms that focus on training on the human genome, deepG can handle sets of (meta)genomes via a range of data augmentation strategies, allowing for the training of highly accurate predictive models on reads, contigs, genomes, or metagenomes.
Cortical thickness and Subcortical volume changes differ between Parkinson disease subtypes
Authors: Huang Mingming , Yu Hui , Guo Kunle , Yin Jianhong , Lei Pinggui , Gao Bo 
Published: February 2023

Abstract

BACKGROUND: To explore the different patterns of cortical thickness and subcortical volume changes between Parkinson disease (PD) subtypes by structural magnetic resonance imaging (MRI).METHODS:We enrolled 48 clinically confirmed PD patients, including 23 tremor dominant (TD) and 25 postural instability gait dominant (PIGD) subtypes, and 32 matched healthy control (HC) volunteers were also collected, all subjects underwent 3.0 Tesla high-resolution structural MRI scanning. Cortical thickness and subcortical volumetric analysis were estimated using an automated Computational Anatomy Toolbox (CAT12) toolbox.Results:Compared with the HC group, PIGD patients had significantly thinning cortical thickness in multiple brain regions, such as bilateral inferiorparietal, paracentral, precuneus, superiorfrontal, caudalmiddlefrontal, posteriocingulate, parahippacampal, lateraloccipital and right superiortemporal, supramarginal and parsorbitals. TD patients had slightly thinning focal cortical thickness in bilateral posteriocingulate, left inferioparietal and right precuneus. PIGD patients had thinner cortical thickness in left caudalmiddlefrontal, parahippacampal and right isthmuscingulate and lateraloccipital than TD patients. In addition, subcortical volume atrophy was identified in the bilateral hippocampus and bilateral amygdala of the patients with PIGD, only right hippocampus changes were found in the TD group. Moreover, the largest area under the ROC curve of hippocampus and atrophy ratio, cortical thickness in region of interest (ROI) and combination of hippocampus ratio, atrophy ratio and cortical thickness in ROIs which for distinguish PIGD from TD were 0.733, 0.912 and 0.999 respectively.Conclusions:Morphometric abnormalities were greater in the PIGD than that in the TD subtype, the disparate patterns of cortical and subcortical degeneration had a potential possibility to distinguish the PD subtypes by MRI perspective in clinical practice.
Unfolding the hydrogen gas sensing mechanism across 2D Pnictogen/Graphene heterostructure sensors
Authors: Kumar Neeraj , Jasani Jaykumar , Sonvane Yogesh , Korvink Jan , Sharma Ashutosh , Sharma Bharat 
Published: February 2023

Abstract
Hydrogen as a source of clean and future alternative to fossil fuels demands a class of powerful gas sensor technologies. To this end, we have developed unique bi-stacked heterogeneous layers based on 2D Pnictogens and graphene sheets via photolithography and pattern transfer methods. The nanoengineered heterostructure morphology consists of truncated/hexagonal antimonene flakes dispersed on graphene sheets. Amongst the Pnictogen class, the hybrid antimonene-graphenes (Sb/G) demonstrated excellent sensing behavior characterized by a large gas response to H2 (~4-fold improvement), high selectivity, fast response/recovery times (>3 fold reduction), and ultra-low limit of detection (LOD: 0.05 ppm) in comparison to pristine sensors. The microstructural and density functional theory (DFT) calculations demonstrated a Bader charge transfer mechanism from Sb/G heterojunction to the H2 gas, which modulated the Schottky barrier and improved gas response. The outstanding performance of the Sb/G sensor places it amongst the highly selective and fast hydrogen sensors based on the Pnictogen-graphene family.
Invasion trends of aquatic Ludwigia hexapetala and L. peploides subsp montevidensis (Onagraceae) in Italy based on herbarium records and global datasets
Authors: Gori Benedetta , Pezzi Giovanna , Brundu Giuseppe , Ceschin Simona , Palella Emanuele , Alessandrini Alessandro , Amadei Lucia , Andreatta Sebastiano , Ardenghi Nicola Maria Giuseppe , Armiraglio Stefano , Bagella Simonetta , Bolpagni Rossano , Bonini Ilaria , Bouvet Daniela , Brancaleoni Lisa , Buccheri Massimo , Buffa Gabriella , Chiarucci Alessandro , Cogoni Annalena , Domina Gianniantonio , Guarino Riccardo , Forte Luigi , Gubellini Leonardo , Guglielmone Laura , Hofmann Nicole , Iberite Mauro , Lastrucci Lorenzo , Lucchese Fernando , Marcucci Rossella , Mei Giacomo , Mossetti Umberto , Nascimbene Juri , Passalacqua Nicodemo Giuseppe , Peccenini Simonetta , Prosser Filippo , Repetto Giovanni , Rinaldi Gabriele , Romani Enrico , Rosati Leonardo , Santangelo Annalisa , Scoppola Anna , Spampinato Giovanni , Stinca Adriano , Tavano Maria , Caruso Fulvio Tomsich , Vangelisti Roberta , Venanzoni Roberto , Vidali Marisa , Vilhalm Thomas , Zonca Francesco , Buldrini Fabrizio , Lambertini Carla 
Published: February 2023

Abstract

Identifying areas susceptible to invasion by an alien species is a strategy of prevention. We used national herbaria and global databases to assess the invasion trends of the two aquatic invasive species Ludwigia hexapetala and Ludwigia peploidessubsp. montevidensis in Italy. We defined the invasion status with invasions curves and predicted potentially suitable areas with Species Distribution Models based on WorldClim variables and the human footprint index. Low seasonal variation in temperature and precipitation, temperature ≥ 20 °C in the warmest, driest and wettest periods of the year and precipitation in the coldest period are the bioclimatic factors that most account for the potential distribution of the two species. The human footprint has lower relative importance than bioclimatic variables. All Italian peninsula appears as a suitable bioclimatic environment for the invasion of the two Ludwigia species, except the Alps and the highest peaks in the Apennine. Based on the current distribution of the species in Italy and the mostly densely invaded areas globally, the agricultural land surrounding the current invaded areas and along the Italian coasts is the most vulnerable to the invasion. Considering the trend of the invasion curves, which have been sharply rising for the latest decades, there are reasons to expect that the alien Ludwigia species will continue their expansion, if no timely and effective actions are taken. Informative campaigns, accurate monitoring and prompt management are fundamental preventive tools in areas predicted as vulnerable to invasion by this study.
Three-dimensional in vivo monitoring of mycobacterial infections and therapeutic efficacy based on tissue-clearing technology CUBIC
Authors: Hakamata Mariko , Nishiyama Akihito , Inouchi Erina , Yokoyama Akira , Kaboso Shaban A. , Gebretsadik Gebremichal , Yamasaki Tomoya , Moro Hiroshi , Ozeki Yuriko , Tateishi Yoshitaka , Ohashi Riuko , Primm Todd P. , Kikuchi Toshiaki , Tainaka Kazuki , Matsumoto Sohkichi 
Published: February 2023

Abstract
Mycobacteria are a continuous threat to human health. They include various species, such as Mycobacterium tuberculosis (M. tuberculosis), which is an intracellular parasite of mammals, and the most virulent and non-tuberculous mycobacteria (NTM), namely, M. avium, which are environmental bacteria causing intractable NTM diseases. An infection model of transparent zebrafish and fish-infectious M. marinum was established to better understand the in vivo behavior of mycobacteria under the pressure of host immune responses. However, the fish model does not fully replicate mammalian immunity. Here, we demonstrate that a clear, unobstructed brain/body imaging cocktail and computational analysis (CUBIC)-based infection (CUBIC-infection) analysis enables comprehensive mycobacterial profiling of the whole lung. We assessed the in vivo kinetics of mycobacterial infection along with fluorescent protein-expressing recombinant mycobacteria. We detected mycobacterium at a single bacterial level and counted bacterial numbers, which was comparable to the colony-forming units of organ homogenates. CUBIC-infection analysis distinguished in vivo spatiotemporal behavior of M. tuberculosis, M. tuberculosisvariant Bacillus Calmette-Guerin, and M. avium in mice. Furthermore, it monitored spatiotemporal information on the therapeutic efficacies of anti-tuberculosis drugs and an anti-lymphangiogenesis agent. Our data suggest that CUBIC-infection analysis is a powerful tool for understanding mycobacterial infections in mammals and developing therapeutic agents.
Optimization of Water and Fertilizer Management of Panax notoginseng Based on Changes in Soil Microbial Biomass Carbon and Nitrogen and Enzyme Activities
Authors: yunfei tuo , xiangning liu , yang zheng , qian Wang , fei Wang , zhaoyi wang , xiaolan shi , mingjing ding , fangyuan shen , ping xiang , qiliang yang 
Published: February 2023

Abstract
At present, there is a lack of understanding of the dynamic characteristics of the carbon, nitrogen, and enzyme activities of the soil microbial biomass of Panax notoginseng under irrigation and fertilization. We set up a field experiment with 3 irrigation and 4 fertilization levels for a total of 12 treatments plus a control in the micro-sprinkler Panax notoginseng farmland in Luxi County, Yunnan Province, from 2018 to 2020. The results showed that the soil microbial biomass carbon, nitrogen, and enzyme activities increased from the rooting period to the flowering period, and then decreased in the fruiting period.The soil microbial biomass carbon, nitrogen, and enzyme activities of Panax notoginseng reached the maximum values in the flowering period, while the minimum values were reached in the rooting period. There were significant positive correlations between the soil microbial biomass carbon and nitrogen and enzyme activities during different growth periods under different water and fertilizer optimization management. This study revealed the tendency of the carbon, nitrogen, and enzyme activities of the soil microbial biomass of Panax notoginseng with the growth periods. It also analyzed the optimal management of different water and fertilizer regimes and the Ci values of soil microbial biomass carbon, nitrogen, and enzyme activities in different growth periods to determine the best water and fertilizer treatment methods in each growth period through the entropy value method combined with the TOPSIS method. The study found that the Ci values changed with the change in the fertilization level under the same irrigation level. The Ci values decreased and then increased with increasing irrigation water under the same fertilization level. These results showed that, in terms of the effect of different water and fertilizer treatments on the regulation of the soil microbial biomass carbon, nitrogen, and enzyme activities in each growth period, the irrigation volume of 10 mm and fertilizer application of 8.00 kg.667− 1.m− 2 during the rooting period of Panax notoginseng were most effective, while an irrigation volume of 15 mm and fertilizer application of 8.00 kg.667− 1.m− 2 were more suitable for the seedling, flowering, and fruiting periods.
Molecular evolutionary model based phylogenetic and mutation analysis of SARS-CoV2 spike protein sequences from Asian countries: a phylogenomic approach
Authors: Said Kamaleldin B , Alsolami Ahmed , Alshammari Fawaz , Alshammari Khalid Farhan , Alazmi Meshari , Bhardwaj Tulika , Najm Mohammad Zeeshan , Singh Rajeev , Kausar Mohd Adnan 
Published: February 2023

Abstract
The lethal pathogenic severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection has caused the COVID-19 pandemic, posing serious risks to people. The clove-like spike (S) protein that distinguishes coronaviruses from other viruses is important for viral pathogenicity, evolution, and transmission. The investigation of the unique structural mutations of the SARS-CoV-2 spike protein among 34 Asian countries, as well as the resulting phylogenetic relationship, provided critical information in understanding the pathogenesis. This can be utilized for the discovery of possible treatments and vaccine development. The current study analysed and depicted phylogenetic and evolutionary models useful for understanding SARS-CoV-2 human-human transmission dynamics in Asian regions with shared land borders. Further, integrated bioinformatics analysis was performed to predict the pathogenic potential and stability of 53 mutational positions among 34 coronavirus strains. Mutations at positions D614G, N969K and S884F have deleterious effects on protein function. These findings are crucial because the Asian mutations could potentially provide a vaccine candidate with co-protection against all SARS-CoV-2 strains. This region is vulnerable because of the high population density and the volume of domestic and international travel for business and tourism. These discoveries would also aid in the development of plans for governments and the general populace to implement all required biocontainment protocols common to all countries.
Sulfite excitation by photolysis to produce reducing species for 4-chloro-2- methylphenoxyacetic acid photo-reduction
Authors: Azarpira Hossein , Rasolevandi Tayebeh , Mahvi Amir Hossein 
Published: February 2023

Abstract
The photo-reduction of MCPA by UV/Sulfite reduction process is the subject of this study. The optimal pH was (MCPA: Sulfite molar ratio of 3:1 and pH 11) after 20 min reaction time. The Impact of In UV/Sulfite process, the pseudo-first-order reaction constant decreases as concentration rises from 50 to 200. mg L− 1, Kobs and robs decreased and reached from 0.299 to 0.029 min− 1 and 14.95 to 5.80 mg L− 1 respectively. Also, the analysis of energy consumption using the kinetic model and IUPAC shows that with the increase in the concentration of MCPA from 50 to 200 mg L− 1, the amount of EEO from 0.821 to 6.54 kwh.m− 3 in the kinetic model and from 0.91 increases to 6.29 kwh.m−3 in the IUPAC model. in the decomposition of MCPA such as 2-prop-1-en-1-yloxyacetic acid (C5H8O3) and 1-ethoxyprop-1-ene (C5H10O) and hexa-1,3,5 -triene (C6H8), converted. These simple compounds have a high potential to become water and carbon dioxide. The improvement of biodegradability was investigated at 30 min reaction time and the amount of photo-reduction of MCPA, COD and TOC were 100%, 35% and 24%, respectively. In order to evaluate the toxicity of UV/Sulfite process effluent, the acute toxicity test by Kirby-Bauer disc was used, results showed that the zone of inhibition surrounding the disk reduced from 31 mm (without treatment) to 5 mm after treatment. As a Conclusion Sulfite/UV process had a good performance in converting toxic materials into degradable organic materials and the effluent toxicity to the environment was very low.