bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosomal rDNA Distribution Patterns in Clonal Cobitis Triploid Hybrids (Teleostei, Cobitidae): Insights into Parental Genomic Contributions
Authors: Boroń Alicja , Grabowska Anna , Jablonska Olga , Kirtiklis Lech , Duda Sara , Juchno Dorota 
Published: January 2025

Background: Interspecific hybridization between relative species Cobitis taenia (with a diploid genome designated as TT), Cobitis elongatoides (EE) and Cobitis tanaitica (NN) and the successive polyploidization with transitions from sexuality to asexuality experienced by triploid Cobitis hybrids likely influence their chromosomal rearrangements, including rearrangements of ribosomal DNA (rDNA) distribution patterns. Previously, we documented distinct karyotypic differences: C. elongatoides exhibited bi-armed chromosomes while C. taenia showed uni-armed chromosomes with rDNA-positive hybridization signals, respectively. Methods: In this study, fluorescence in situ hybridization (FISH) with 5S rDNA and 28S rDNA probes was used to analyze and compare chromosomal distribution patterns of rDNAs in clonally reproduced triploid Cobitis hybrids of different genomic constitutions ETT, ETN, EEN and EET (referred to using acronyms denoting the haploid genomes of their parent species), and their parental species. Results: Cobitis triploid hybrids exhibited intermediate karyotypes with ribosome synthesis sites on chromosomes inherited from both parents, showing no evidence of nucleolar dominance. The rDNA pattern derived from the C. elongatoides genome was more stable in the hybrids’ karyotypes. Two and one submetacentric chromosomes with co-localized rDNAs were effective markers to ascertain C. elongatoides diploid (EE) and haploid (E) genomes within the genome of triploid hybrids, respectively. Fewer 5S rDNA loci were observed in diploid (TT) and haploid (T) chromosome sets from C. taenia in ETT and EET females. C. taenia and C. tanaitica exhibited similar rDNA distribution patterns. Conclusions: The karyotypes of triploid Cobitis hybrids reflect the genomic contributions of their parental species. Variability in rDNA distribution patterns suggests complex genomic interactions in Cobitis hybrids resulting from polyploidization and hybridization, potentially influencing their reproductive potential.
Multi-Dimensional Markov Chains of M/G/1 Type
Authors: Naumov Valeriy , Samouylov Konstantin 
Published: January 2025

We consider an irreducible discrete-time Markov process with states represented as (k, i) where k is an M-dimensional vector with non-negative integer entries, and i indicates the state (phase) of the external environment. The number n of phases may be either finite or infinite. One-step transitions of the process from a state (k, i) are limited to states (n, j) such that n ≥ k−1, where 1 represents the vector of all 1s. We assume that for a vector k ≥ 1, the one-step transition probability from a state (k, i) to a state (n, j) may depend on i, j, and n − k, but not on the specific values of k and n. This process can be classified as a Markov chain of M/G/1 type, where the minimum entry of the vector n defines the level of a state (n, j). It is shown that the first passage distribution matrix of such a process, also known as the matrix G, can be expressed through a family of nonnegative square matrices of order n, which is a solution to a system of nonlinear matrix equations.
Application of Principal Component Analysis as a Prediction Model for Feline Sporotrichosis
Authors: Pegoraro Franco Bresolin , Mangrich-Rocha Rita Maria Venâncio , Weber Saulo Henrique , de Farias Marconi Rodrigues , Schmidt Elizabeth Moreira dos Santos 
Published: January 2025

Sporotrichosis is a worldwide zoonotic disease that is spreading and causing epidemics in large urban centers. Cats are the most susceptible species to develop the disease, which could cause significant systemic lesions. The aim was to investigate and to identify predictive indicators of disease progression by correlations between the blood profile (hematological and biochemical analytes) and cutaneous lesion patterns of 70 cats diagnosed with Sporothrix brasiliensis. The higher occurrence in male cats in this study could be related to being non-neutered and having access to open spaces. Principal component analysis (PCA) with two principal components, followed by binary logistic regression, and binary logistic regression analysis, with independent variables and backward elimination modeling, were performed to evaluate hematological (n = 56) and biochemical (n = 34) analytes, including red blood cells, hemoglobin, hematocrit, leukocytes, segmented neutrophils, band neutrophils, eosinophils, lymphocytes, monocytes, total plasma protein, albumin, urea, creatinine, and alanine aminotransferase. Two logistic regression models (PCA and independent variables) were employed to search for a predicted model to correlate fixed (isolated) and disseminated cutaneous lesion patterns. Total plasma protein concentration may be assessed during screening diagnosis as it has been recognized as an independent predictor for the dissemination of cutaneous lesion patterns, with the capability of serving as a predictive biomarker to identify the progression of cutaneous lesions induced by S. brasiliensis infections in cats.
Association between video consultation use and general practitioner and practice characteristics: a cross-sectional survey in Denmark
Authors: Prætorius Thim , Klausen Fredslund Eskild , Torp Daniel Cæsar , Sandbaek Annelli 
Published: January 2025

BackgroundVideo consultations (VC) disrupt how general practice provides care and how patients receive it. A step towards understanding the use of VC is to study the association between user-status and general practitioner and practice characteristics.AimTo study the association between general practitioner and general practice characteristics and VC user-status (users, never users, and former users).Design & settingAn anonymous, web-based, cross-sectional survey was distributed to all 1674 Danish general practices (singlehanded, collaborative, and partnership forms) contracting with and working on a collective agreement with the public funder.MethodMultinomial logistic regression was used to correlate VC user-status and (1) general practice characteristics, and general practitioners' (2a) objective characteristics and (2b) subjective attitudes towards VC and organizational change.ResultsThe study sample included 416 general practitioners. Users of VC compared to never-users: partnership practices (RRR=0.22; 95% CI 0.06-0.85) and practices with six or more practice staff (RRR=0.05; 95% CI 0.01-0.28) were significantly more likely to be users. The same was found for general practitioners with a high degree of tech savviness (RRR=0.02; 95% CI 0.001-0.17) and openness to organisational change (RRR=0.26; 95% CI 0.08-0.85).ConclusionCharacteristics of general practice and general practitioners are associated with VC user-status (being a user, never user or former user). Future research should use a Difference-in-Difference study design and register data to make causality claims.
PENGARUH IMBANGAN TAPE KETAN HITAM DAN BUAH NAGA MERAH TERHADAP MUTU ORGANOLEPTIK PADA SNACK BAR DIABETES MELITUS
Authors: Hapsari Agustina Indri , Widartika Widartika 
Published: January 2025

Oxidative stress in DM patients can be inhibited by consuming foods that are high in antioxidants. Black glutinous tape and red dragon fruit were chosen as snack bar ingredients because they contain high fiber, low Glycemic Index (IG), and high antioxidants. The study aimed to analyze the organoleptic quality of the formulation of black glutinous rice tape snack bars and red dragon fruit. The design of this study was experimental with a Complete Random Design (RAL) consisting of independent variables and dependent variables. The independent variable was the balance of black sticky rice tape: red dragon fruit F1=75:25, F2=60:40, and F3=50:50. The dependent variable is the organoleptic quality of the Snack Bar DM product. The hedonic test involved 30 moderately trained panelistsThe analysis used was the One-Way Anova test and the Duncan follow-up test. The results showed that there was no difference in terms of color, aroma and texture in the three black glutinous rice snack bar and red dragon fruit snack bar formulas. However, there were organoleptic differences in taste in various balances of black glutinous rice tape snack bars and red dragon fruit p=0.032. Duncan's test results show that the best snack bar flavor is a snack bar with formula 3 with a balance of 50% black glutinous tape: 50% red dragon fruit.
Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell–Cell Fusion and Immune Activation
Authors: Wang Yao , Pang Xunlei , Li Ruirui , Chen Jiuzhou , Wen Chen , Zhu Huihuang , Long Tingyu , Li Jianjie , Zheng Lijun , Deng Youcai , Zheng Junnian , Xu Bo 
Published: January 2025

Background:
Chimeric antigen receptor (CAR)-based immune cell therapies attack neighboring cancer cells after receptor recognition but are unable to directly affect distant tumor cells. This limitation may contribute to their inefficiency in treating solid tumors, given the restricted intratumoral infiltration and immunosuppressive tumor microenvironment. Therefore, cell–cell fusion as a cell-killing mechanism might develop a novel cytotherapy aimed at improving the efficacy against solid tumors.
Methods:
We constructed a fusogenic protein, fusion-associated small transmembrane (FAST) p14 of reptilian reovirus, into cancer cells and mesenchymal stem cells (MSCs), which cocultured with various colon cancer cells and melenoma cells to validate its ability to induce cell fusion and syncytia formation. RNA sequencing, quantitative reverse transcription polymerase chain reaction, and Western blot were performed to elucidate the mechanism of syncytia death. Cell viability assay was employed to assess the killing effects of MSCs carrying the p14 protein (MSCs-p14), which was also identified in the subcutaneous tumor models. Subsequently, the Tet-On system was introduced to enhance the controllability and safety of therapy.
Results:
Cancer cells incorporated with fusogenic protein p14 FAST from reovirus fused together to form syncytia and subsequently died through apoptosis and pyroptosis. MSCs-p14 cocultured with different cancer cells and effienctly induced cancer cell fusion and caused widespread cancer cell death in vitro. In mouse tumor models, mMSCs-p14 treatment markedly suppressed tumor growth and also enhanced the activity of natural killer cells and macrophages. Controllability and safety of MSCs-p14 therapy were further improved by introducing the tetracycline-controlled transcriptional system.
Conclusion:
MSC-based cytotherapy carrying viral fusogenic protein in this study kills cancer cells by inducing cell–cell fusion. It has demonstrated definite efficacy in treating solid tumors and is worth considering for clinical development.
ИГРЫ КОМИ
Authors: Ключева Мария Аркадьевна 
Published: January 2025

Рец. на: Детский фольклор коми: игры / автор-составитель А. Н. Рассыхаев. Сыктывкар: ФИЦ Коми НЦ УрО РАН, 2023. 524 с.
Review of: Children's folklore of the Komi: games. Сompiled by Aleksey Rassykhaev. Syktyvkar: FRC Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, 2023. 524 p.
СИЯНИЕ ХРУПКОГО МИРА
Authors: Рогачев Владимир Ильич , Карабанова Надежда Валериевна 
Published: January 2025

Рецензия на: Динисламова С. С. Моя Сосьва река: сборник рассказов на русском языке / С. С. Динисламова; предисл. И. А. Ширманов. - Тюмень, ООО «ФОРМАТ-72», 2023. - 134 с.
Review of: Dinislamova S. S. My Sosva River: a collection of short stories in Russian / S. S. Dinislamova; preface by I. A. Shirmanov. Tyumen, FORMAT-72, 2023. 134 p.
A saúde como tema na educação física escolar
Authors: Bisconsini Camila Rinaldi , Rinaldi Wilson , Barbosa-Rinaldi Ieda Parra 
Published: January 2025

A pesquisa objetivou analisar a viabilidade do desenvolvimento da temática saúde nas aulas de educação física escolar no ensino médio, a partir do proposto pelas Diretrizes Curriculares do Estado do Paraná. O estudo tem como método de investigação a pesquisa-ação. Conclui-se que é possível trabalhar a temática saúde de forma ampla na escola, permitindo que os alunos entendam que o corpo, a atividade física e a sociedade estão intimamente relacionados e que a saúde pode ser um resultado das ações do ser humano desde que se considere os diversos determinantes que se relacionam com ela. Por fim, com esse estudo esperamos contribuir com a presença do trato com o tema saúde na educação física escolar de maneira crítica.
Prevenção de afogamentos infantis: Estratégias essenciais e a aplicação da cadeia de sobrevivência
Authors: Alvarenga Jais Monteiro Cordeiro de , Gomes Amanda , Santos Amanda Beatriz da Silva Cerqueira , Menegace Diego de Menezes , Sposito Raffaella Azevedo , Freitas Laura de Souza , Victoretti Isabela Araújo , Silva Demetrius de Souza e , Santos Priscila Parente , Sousa Julia Lino , Marinho Maria Eduarda Clemente da Silva , Souza Jorge Augusto Soares de 
Published: January 2025

Os afogamentos representam uma das principais causas de morte evitável em crianças. A prevenção do afogamento infantil requer a implementação de estratégias eficazes, sendo que, no âmbito da saúde pública, deve ser uma prioridade a fim de reduzir os índices de mortalidade e de sequelas graves. Este artigo tem como objetivo discutir as estratégias na prevenção de afogamentos infantis, além da importância da aplicação adequada da cadeia de sobrevivência do afogamento. O estudo foi realizado através de uma busca nas bases de dados Medline/PubMed, Biblioteca Virtual em Saúde (BVS) e Periódicos CAPES. A questão de pesquisa e a estratégia utilizadas foram baseadas no modelo PICO. Foram incluídos estudos de ensaio clínico, meta-análise, ensaio controlado randomizado, artigos completos, livros e documentos, publicados nos idiomas inglês, espanhol e português, nos últimos 5 anos. Foram encontrados um total de 220 artigos inicialmente. Após as análises e as eliminações das duplicatas, foram analisadas 179 referências por título e resumo, das quais 147 foram incluídas para leitura na íntegra. Após essa etapa, 135 referências foram excluídas. Ao final, 12 estudos foram considerados elegíveis. As evidências disponíveis demonstram que a prevenção de afogamentos infantis exige uma abordagem multifacetada que integra conscientização, supervisão ativa e o fortalecimento de estratégias de segurança. Além disso, aplicar a cadeia de sobrevivência é essencial para aumentar as chances de salvar vidas, destacando a importância de uma resposta rápida e coordenada.
MONJARO (TIRZEPATIDA): BENEFÍCIOS E MALEFÍCIOS NO TRATAMENTO DO DIABETES TIPO 2 E OBESIDADE
Authors: Cunha Christian Pannain da , Martins Hosana Maria Rodrigues Lobo , Sousa Sávia Suélly Fernandes de , Carvalho Maria Júlia de , Semkiw Michel Roberto Publitz , Bandeira Luiz Fernando de Souza , Cordeiro Júnior José Zito Alves , Kniphoff Iasmim de Souza Oliveira , Oliveira Luana de Souza , Guimarães Kaique Wbiratan Rocha , Alencar Renato Kaio Morais de 
Published: January 2025

Introdução: A prevalência crescente do diabetes tipo 2 e da obesidade constitui um desafio significativo para a saúde pública global, exigindo o desenvolvimento de terapias eficazes e seguras. A tirzepatida, comercialmente conhecida como Monjaro, emergiu como uma opção terapêutica promissora devido à sua ação dual nos receptores de GLP-1 e GIP, hormônios incretínicos essenciais na regulação da glicose e controle do apetite. Objetivo: Esta revisão bibliográfica tem como objetivo analisar os benefícios e malefícios da tirzepatida no tratamento do diabetes tipo 2 e da obesidade, com base nas evidências científicas mais recentes. Métodos: Foi conduzida uma busca sistemática na literatura científica entre 2013 e 2023, utilizando as bases de dados PubMed, Scopus e Google Scholar. Foram incluídos estudos originais e revisões publicadas em inglês, português ou espanhol, focados nos aspectos diagnósticos, impactos na saúde mental e estratégias terapêuticas relacionadas ao uso da tirzepatida. Após a triagem, 25 estudos foram selecionados para a análise. Resultados e Discussão: A tirzepatida demonstrou eficácia significativa no controle glicêmico, reduzindo a hemoglobina A1c e a glicemia pós-prandial, além de promover uma perda de peso corporal substancial em comparação com outras terapias antidiabéticas. O perfil de segurança da tirzepatida é aceitável, embora associada a efeitos adversos gastrointestinais como náuseas e vômitos. Comparada a outras terapias, a tirzepatida oferece vantagens em termos de eficácia e abrangência terapêutica, justificando a importância desta revisão para consolidar as evidências disponíveis e orientar a prática clínica. Conclusão: A tirzepatida apresenta um potencial significativo como terapia dual no manejo do diabetes tipo 2 e da obesidade, com benefícios terapêuticos que superam os riscos para a maioria dos pacientes. Contudo, é essencial o monitoramento contínuo e a personalização da terapia para otimizar os resultados clínicos e garantir a segurança do tratamento. A continuidade das pesquisas é fundamental para aprofundar o entendimento sobre os efeitos a longo prazo da tirzepatida e maximizar seus benefícios no contexto clínico.
TRANSTORNO DE DÉFICIT DE ATENÇÃO E HIPERATIVIDADE (TDAH) EM ADULTOS: DIAGNÓSTICO, IMPACTOS NA SAÚDE MENTAL E ESTRATÉGIAS TERAPÊUTICAS
Authors: Cunha Christian Pannain da , Martins Hosana Maria Rodrigues Lobo , Sousa Sávia Suélly Fernandes de , Carvalho Maria Júlia de , Semkiw Michel Roberto Publitz , Bandeira Luiz Fernando de Souza , Cordeiro Júnior José Zito Alves , Kniphoff Iasmim de Souza Oliveira , Oliveira Luana de Souza , Guimarães Kaique Wbiratan Rocha , Alencar Renato Kaio Morais de 
Published: January 2025

Introdução: O Transtorno de Déficit de Atenção e Hiperatividade (TDAH) em adultos é uma condição neuropsiquiátrica que impacta significativamente a vida funcional e a saúde mental, mas ainda enfrenta desafios diagnósticos e terapêuticos. Objetivo: Esta revisão bibliográfica visa sintetizar as evidências recentes sobre o diagnóstico, os impactos na saúde mental e as estratégias terapêuticas para o TDAH em adultos, destacando a importância de intervenções personalizadas e integradas. Métodos: Foi realizada uma busca sistemática na literatura científica utilizando as bases de dados PubMed, Scopus e Google Scholar, cobrindo o período de 2009 a 2024. Foram incluídos estudos originais e revisões publicados em inglês, português ou espanhol, focados nos aspectos diagnósticos, impactos na saúde mental e estratégias terapêuticas do TDAH em adultos. Após a triagem inicial de 1.500 artigos, 28 estudos foram incluídos na revisão. Resultados e Discussão: A prevalência do TDAH em adultos varia entre 2,5% e 4,5%, com desafios diagnósticos decorrentes da sobreposição de sintomas com outras condições psiquiátricas. Comorbidades como depressão e ansiedade são comuns, complicando o tratamento e exigindo abordagens integradas. O impacto funcional e social inclui dificuldades no emprego e relacionamentos interpessoais, ressaltando a necessidade de intervenções que promovam habilidades sociais e profissionais. Estratégias terapêuticas eficazes combinam intervenções farmacológicas e psicossociais, com destaque para a terapia cognitivo-comportamental e o uso de tecnologias digitais e mindfulness. Conclusão: A revisão enfatiza a necessidade de diagnósticos precisos, gestão das comorbidades, mitigação dos impactos funcionais e sociais, e a evolução das estratégias terapêuticas para melhorar a qualidade de vida dos adultos com TDAH. A integração de abordagens personalizadas e inovadoras é crucial para um manejo clínico eficaz.
„Nie jestem przygotowany, ale jestem gotów”. Głosy z celi śmierci
Authors: Czaja Dariusz 
Published: January 2025

Śmierć przychodzi znienacka, niepodobna się do niej przygotować. Młodość nic nie wie o śmierci, bliżej myślenia o niej jest wiek podeszły, starość – egzystencja, której głęboki sens streszcza się w celnej poetyckiej frazie Tadeusza Różewicza: „cela śmierci”. W swoim tekście Autor pyta o możliwość przygotowania do własnej śmierci, a przedmiotem rozprawy czyni arcydzieło Wiktora Hugo, Ostatni dzień skazańca (Le Derniere Jour d’un condamné, 1829), którego „akcja” dzieje się w realnej celi śmierci. W zakończeniu zestawia swoje uwagi o nieprzygotowaniu do śmierci z opowiadaniem Lwa Tołstoja, Śmierć Iwana Iljicza, w którym śledzi heideggerowską przestrzeń „się” (das Man), oplatającą z wielu stron bohatera opowiadania.
PENGARUH BEBAN KERJA TERHADAP STRES KERJA DENGAN JENIS KELAMIN SEBAGAI MODERATOR PADA KARYAWAN
Authors: Mareta Ninda Ardilla  , Idha Rahayuningsih  
Published: January 2025

Penelitian ini mengkaji jenis kelamin sebagai moderator pengaruh beban kerja terhadap stres kerja pada karyawan di swalayan x. Pada penelitian ini menggunakan metode kuantitatif dengan jumlah sampel sebanyak 53 karyawan dengan teknik non probability sampling. Teknik pengumpulan data dengan menyebarkan kuesioner sebagai instrumen penelitian dengan skala beban kerja dan skala stres kerja. Teknik analisis data menggunakan regresi linier berganda dengan uji hipotesis menggunakan analisis regresi moderasi. Hasil penelitian menunjukkan: 1) ada pengaruh beban kerja terhadap stres kerja pada karyawan di swalayan x. Nilai koefisien Rsquare 0,645 berarti terdapat hubungan yang cukup kuat antara pengaruh variabel beban kerja terhadap variabel stres kerja sebesar 64,5% dan sisanya sebesar 35,5% dipengaruhi oleh variabel lain.; 2) jenis kelamin tidak memoderatori pengaruh beban kerja terhadap stres kerja pada karyawan swalayan x. hal tersebut dapat disebabkan oleh hal yang tidak diperhatikan seperti: jumlah sampel yang tidak seimbang antara laki-laki dan perempuan, karakteristik pekerjaan, dan masa kerja.

Kata kunci : Beban Kerja, Stres Kerja, Jenis Kelamin dan Moderasi.
Moderasi Beragama antara Islam dan Tionghoa di Pulau Bangka
Authors: Irawan  , Nasrun  
Published: January 2025

Abstrak: Artikel ini bertujuan untuk menjelaskan moderasi beragama antara Islam dan Tionghoa di Sungailiat Bangka. Artikel ini dikategorikan sebagai penelitian lapangan (field research) dan dianalisis dengan metode deskriptif kualitatif. Pendekatan yang digunakan dalam artikel ini adalah pendekatan sosiologis historis. Dalam menganalisis hasil penelitian digunakan teori dari Milton M. Gordon: multi-stages of assimilation dan teori dari Peter L. Berger: the sacred canopy (langit suci). Artikel ini menyimpulkan bahwa moderasi Islam antara Islam dan Tionghoa di Bangka berjalan dengan baik. Hal ini disebabkan oleh sejarah, asimilasi (asimilasi struktural, asimilasi kultural, dan asimilasi reseptif), eksternalisasi, objektivasi, dan internalisasi.

Kata kunci: Islam, Tionghoa, moderasi beragama.