bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discrepancy in responses to the surprise question between hemodialysis nurses and physicians, with focus on patient clinical characteristics: A comparative study
Authors: Wallin Jeanette M. , Jacobson Stefan H. , Axelsson Lena , Lindberg Jenny , Persson Carina I. , Stenberg Jenny , Wennman‐Larsen Agneta 
Published: June 2023

AbstractIntroductionThe surprise question (SQ) “Would I be surprised if this patient died within the next xx months” can be used by different professions to foresee the need of serious illness conversations in patients approaching end of life. However, little is known about the different perspectives of nurses and physicians in responses to the SQ and factors influencing their appraisals. The aim was to explore nurses' and physicians' responses to the SQ regarding patients on hemodialysis, and to investigate how these answers were associated with patient clinical characteristics.MethodsThis comparative cross‐sectional study included 361 patients for whom 112 nurses and 15 physicians responded to the SQ regarding 6 and 12 months. Patient characteristics, performance status, and comorbidities were obtained. Cohen's kappa was used to analyze the interrater agreement between nurses and physicians in their responses to the SQ and multivariable logistic regression was applied to reveal the independent association to patient clinical characteristics.FindingsProportions of nurses and physicians responding to the SQ with “no, not surprised” was similar regarding 6 and 12 months. However, there was a substantial difference concerning which specific patient the nurses and physicians responded “no, not surprised”, within 6 (κ = 0.366, p < 0.001, 95% CI = 0.288–0.474) and 12 months (κ = 0.379, p < 0.001, 95% CI = 0.281–0.477). There were also differences in the patient clinical characteristics associated with nurses' and physicians' responses to the SQ.DiscussionNurses and physicians have different perspectives in their appraisal when responding to the SQ for patients on hemodialysis. This may reinforce the need for communication and discussion between nurses and physicians to identify the need of serious illness conversations in patients approaching the end of life, in order to adapt hemodialysis care to patient preferences and needs.
ELECTORAL MALDISTRICTING
Authors: Gomberg Andrei , Pancs Romans , Sharma Tridib 
Published: June 2023

AbstractWe introduce a framework to theoretically and empirically examine electoral maldistricting—the intentional drawing of electoral districts to advance partisan objectives, compromising voter welfare. We identify the legislatures that maximize voter welfare and those that maximize partisan goals, and incorporate them into a maldistricting index. This index measures the intent to maldistrict by comparing distances from the actual legislature to the nearest partisan and welfare‐maximizing legislatures. Using 2008 presidential election data and 2010 census‐based district maps, we find a Republican‐leaning bias in district maps. Our index tracks court rulings in intuitive ways.
RNA sequencing suggests that non‐coding RNAs play a role in the development of acquired haemophilia
Authors: Tigu Adrian Bogdan , Hotea Ionut , Drula Rares , Zimta Alina‐Andreea , Dirzu Noemi , Santa Maria , Constantinescu Catalin , Dima Delia , Bergthorsson Jon Thor , Greiff Victor , Gulei Diana , Coriu Daniel , Serban Margit , Mahlangu Johnny , Tomuleasa Ciprian 
Published: June 2023

AbstractAcquired haemophilia (AH) is a rare disorder characterized by bleeding in patients with no personal or family history of coagulation/clotting‐related diseases. This disease occurs when the immune system, by mistake, generates autoantibodies that target FVIII, causing bleeding. Small RNAs from plasma collected from AH patients (n = 2), mild classical haemophilia (n = 3), severe classical haemophilia (n = 3) and healthy donors (n = 2), for sequencing by Illumina, NextSeq500. Based on bioinformatic analysis, AH patients were compared to all experimental groups and a significant number of altered transcripts were identified with one transcript being modified compared to all groups at fold change level. The Venn diagram shows that haemoglobin subunit alpha 1 was highlighted to be the common upregulated transcript in AH compared to classical haemophilia and healthy patients. Non‐coding RNAs might play a role in AH pathogenesis; however, due to the rarity of HA, the current study needs to be translated on a larger number of AH samples and classical haemophilia samples to generate more solid data that can confirm our findings.
Immunoinformatics aided design of a peptide‐based kit for detecting Escherichia coliO157:H7 from food sources
Authors: Oladipo Elijah Kolawole , Oluwasegun Jerry Ayobami , Oladunni Tolu David , Owoeye Victoria Oluwakemi , Obisanya Olukayode Ifeanyi , Olasinde Oluwatosin Tumininu , Onyeaka Helen 
Published: June 2023

AbstractFood and water‐borne enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is a zoonotic bacterium that causes gastroenteritis and other human diseases. It has also been linked to chronic foodborne diseases with high mortality rates worldwide, particularly in children. Hence, this study was carried out to designed a peptide base kit for quick detection of E. coli in food. A peptide‐based rapid detection kit was designed using an immunoinformatic technique and some antigenic target genes (stx1A, stx2B, escC, fliC, and eae). The antigenic gene sequences retrieved were screened for antigenicity, transmembrane topology, B‐cells and helper T‐cells. Selected epitopes were joined with appropriate linkers to form a chimeric protein which consists of five B‐cell epitopes, five interleukin‐4 (IL‐4) inducer epitopes and five interleukin‐10 (IL‐10) inducer epitopes. The improved and optimized chimeric protein sequence was cloned in‐silico in a suitable expression host, E. coli‐strain K12. The designed peptide refined and validated tertiary structure was molecularly docked with the tertiary structures of each antigenic target gene. The physicochemical properties of the chimeric protein showed that the construct has an amino acid length of 295 amino acids, a molecular weight of 29.876 kiloDalton (kDa), an aliphatic index of 75.05 and an instability index of 14.82 which confers stability. The construct was hydrophilic with a GRAVY value of −0.261 and had a considerable half‐life of 4.4 h (mammalian reticulocytes, in vitro), >20 h (yeast, in vivo) and >10 h (E. coli, in vivo). Conclusively, the final construct has successfully met the design requirements for the development of a lateral flow kit, which has the potential to provide fast and efficient detection of E. coli O157:H7. However, it is the additional validation through the vitro and in vivo techniques needed to confirm that this designed peptide based test kit.
Case report: Novel leg vein treatment CLaCS guided by augmented reality 11 years follow‐up
Authors: Miyake Kasuo , Grill Marcelo Halfen , Feitoza Heidi Shiho Nagatani 
Published: June 2023

AbstractTelangiectasias is most prevalent on the lower limbs and has been estimated to manifest in 40%–90% of the population. Treatments for telangiectasias include sclerotherapy, laser therapy, intense pulsed light treatment, microphlebectomy, and thermocoagulation. Cryo‐Laser & Cryo‐Sclerotherapy (CLaCS) effectively combines thermal and injection sclerotherapy. In this treatment, unwanted veins are targeted by a transdermal laser and immediately receive injection sclerotherapy. During the whole procedure, an air‐cooling device (Cryo) blows onto the surrounding skin and tissue to prevent skin burn. Here, we present a case report of a challenging telangiectasias treated with ClaCS.
Stealth Acquisitions and Product Market Competition
Authors: KEPLER JOHN D. , NAIKER VIC , STEWART CHRISTOPHER R. 
Published: June 2023

ABSTRACTWe examine whether and how firms structure their merger and acquisition deals to avoid antitrust scrutiny. There are approximately 40% more mergers and acquisitions (M&As) than expected just below deal value thresholds that trigger antitrust review. These “stealth acquisitions” tend to involve financial and governance contract terms that afford greater scope for negotiating and assigning lower deal values. We also show that the equity values, gross margins, and product prices of acquiring firms and their competitors increase following such acquisitions. Our results suggest that acquirers manipulate M&As to avoid antitrust scrutiny, thereby benefiting their own shareholders but potentially harming other corporate stakeholders.
SIK2: A critical glucolipid metabolic reprogramming regulator and potential target in ovarian cancer
Authors: Hu Dan , Du JunHong , Xing YiJuan , Cheng YueMei , He RuiFen , Liang XiaoLei , Li HongLi , Yang YongXiu 
Published: June 2023

AbstractAimTo explore the role of salt‐inducible kinase 2 (SIK2) on glucose and lipid metabolism in ovarian cancer (OC), so as to increase the understanding of potential inhibitors targeting SIK2 and lay a foundation for future precision medicine in OC patients.MethodsWe reviewed and summarized the regulation effect of SIK2 on glycolysis, gluconeogenesis, lipid synthesis, and fatty acids β‐oxidation (FAO) in OC, as well as the potential molecular mechanism and the prospects of potential inhibitors targeting SIK2 in future cancer treatments.ResultsMany pieces of evidence show that SIK2 is closed associated with glucose and lipid metabolism of OC. On the one hand, SIK2 enhances the Warburg effect by promoting glycolysis and inhibiting oxidative phosphorylation and gluconeogenesis, on the other hand, SIK2 regulates intracellular lipid metabolism through promoting lipid synthesis and FAO, all of which ultimately induces growth, proliferation, invasion, metastasis, and therapeutic resistance of OC. On this basis, SIK2 targeting may become a new solution for the treatment of a variety of cancer types including OC. The efficacy of some small molecule kinase inhibitors has also been demonstrated in tumor clinical trials.ConclusionSIK2 displays significant effects in OC progression and treatment through regulating cellular metabolism including glucose and lipid metabolism. Therefore, future research needs to further explore the molecular mechanisms of SIK2 in other types of energy metabolism in OC, based on this to develop more unique and effective inhibitors.
The Concept of Function in Social Positioning Theory
Authors: Pratten Stephen 
Published: June 2023

AbstractThe term function currently features prominently in outlines of social positioning theory but a sustained account of the view of function informing social positioning theory has yet to be supplied. In the absence of a fuller articulation of the theory's underlying view of function confusion and misinterpretation are likely to be encouraged especially among those committed to one or other of the numerous alternative accounts of function available. In this paper key features of the concept of function as it now appears in social positioning theory are identified and the similarities and differences between it and selected other views of function explored.
Deep diving by offshore bottlenose dolphins (Tursiops spp.)
Authors: Fahlman Andreas , Tyson Moore Reny B. , Stone Rae , Sweeney Jay , Trainor Robyn Faulkner , Barleycorn Aaron A. , McHugh Katherine , Allen Jason B. , Wells Randall S. 
Published: June 2023

AbstractWe used satellite‐linked tags to evaluate dive behavior in offshore bottlenose dolphins (Tursiops spp.) near the island of Bermuda. The data provide evidence that bottlenose dolphins commonly perform both long (>272 s) and deep (>199 m) dives, with the deepest and longest dives being to 1,000 m and 826 s (13.8 min), respectively. The data show a relationship between dive duration and dive depth for dives longer than about 272 s. There was a diurnal pattern to dive behavior, with most dives deeper than 50 m being performed at night; deep diving began at sunset and varied throughout the night. We used the cumulative frequency of dive duration to estimate a behavioral aerobic dive limit (bADL) of around 560–666 s (9.3–11.1 min) in adult dolphins in this population. Dives exceeding the bADL spent significantly longer time in the upper‐most 50 m following a dive as compared with dives less than the bADL. We conclude that the offshore ecotype off Bermuda, unlike the shallow‐diving near‐shore bottlenose dolphin, is a deep‐diving ecotype, and may provide a useful animal model to study extreme diving behavior and adaptations.
microRNA‐based predictor for diagnosis of frontotemporal dementia
Authors: Magen Iddo , Yacovzada Nancy‐Sarah , Warren Jason D. , Heller Carolin , Swift Imogen , Bobeva Yoana , Malaspina Andrea , Rohrer Jonathan D. , Fratta Pietro , Hornstein Eran 
Published: June 2023

AbstractAimsThis study aimed to explore the non‐linear relationships between cell‐free microRNAs (miRNAs) and their contribution to prediction of Frontotemporal dementia (FTD), an early onset dementia that is clinically heterogeneous, and too often suffers from delayed diagnosis.MethodsWe initially studied a training cohort of 219 subjects (135 FTD and 84 non‐neurodegenerative controls) and then validated the results in a cohort of 74 subjects (33 FTD and 41 controls).ResultsOn the basis of cell‐free plasma miRNA profiling by next generation sequencing and machine learning approaches, we develop a non‐linear prediction model that accurately distinguishes FTD from non‐neurodegenerative controls in ~90% of cases.ConclusionsThe fascinating potential of diagnostic miRNA biomarkers might enable early‐stage detection and a cost‐effective screening approach for clinical trials that can facilitate drug development.
A centronuclear myopathy‐causing mutation in dynamin‐2 disrupts neuronal morphology and excitatory synaptic transmission in a murine model of the disease
Authors: Arriagada‐Diaz Jorge , Flores‐Muñoz Carolina , Gómez‐Soto Bárbara , Labraña‐Allende Marjorie , Mattar‐Araos Michelle , Prado‐Vega Lorena , Hinostroza Fernando , Gajardo Ivana , Guerra‐Fernández María José , Bevilacqua Jorge A. , Cárdenas Ana M. , Bitoun Marc , Ardiles Alvaro O. , Gonzalez‐Jamett Arlek M. 
Published: June 2023

AbstractAimsDynamin‐2 is a large GTPase, a member of the dynamin superfamily that regulates membrane remodelling and cytoskeleton dynamics. Mutations in the dynamin‐2 gene (DNM2) cause autosomal dominant centronuclear myopathy (CNM), a congenital neuromuscular disorder characterised by progressive weakness and atrophy of the skeletal muscles. Cognitive defects have been reported in some DNM2‐linked CNM patients suggesting that these mutations can also affect the central nervous system (CNS). Here we studied how a dynamin‐2 CNM‐causing mutation influences the CNS function.MethodsHeterozygous mice harbouring the p.R465W mutation in the dynamin–2 gene (HTZ), the most common causing autosomal dominant CNM, were used as disease model. We evaluated dendritic arborisation and spine density in hippocampal cultured neurons, analysed excitatory synaptic transmission by electrophysiological field recordings in hippocampal slices, and evaluated cognitive function by performing behavioural tests.ResultsHTZ hippocampal neurons exhibited reduced dendritic arborisation and lower spine density than WT neurons, which was reversed by transfecting an interference RNA against the dynamin‐2 mutant allele. Additionally, HTZ mice showed defective hippocampal excitatory synaptic transmission and reduced recognition memory compared to the WT condition.ConclusionOur findings suggest that the dynamin‐2 p.R465W mutation perturbs the synaptic and cognitive function in a CNM mouse model and support the idea that this GTPase plays a key role in regulating neuronal morphology and excitatory synaptic transmission in the hippocampus.
Treatment with the copper compound CuATSM has no significant effect on motor neuronal pathology in patients with ALS
Authors: Yang Yue , Rowe Dominic , McCann Heather , Shepherd Claire E. , Kril Jillian J. , Kiernan Matthew C. , Halliday Glenda M. , Tan Rachel H. 
Published: June 2023

AbstractAimsAlthough the orally available brain‐penetrant copper compound CuATSM has demonstrated promising effects in SOD1‐linked mouse models, the impact of CuATSM on disease pathology in patients with amyotrophic lateral sclerosis (ALS) remains unknown.MethodsThe present study set out to address this deficit by performing the first pilot comparative analysis of ALS pathology in patients that had been administered CuATSM and riluzole [N = 6 cases composed of ALS‐TDP (n = 5) and ALS‐SOD1 (n = 1)] versus riluzole only [N = 6 cases composed of ALS‐TDP (n = 4) and ALS‐SOD1 (n = 2)].ResultsOur results revealed no significant difference in neuron density or TDP‐43 burden in the motor cortex and spinal cord of patients that had received CuATSM compared with patients that had not. In patients that had received CuATSM, p62‐immunoreactive astrocytes were observed in the motor cortex and reduced Iba1 density was found in the spinal cord. However, no significant difference in measures of astrocytic activity and SOD1 immunoreactivity was found with CuATSM treatment.DiscussionThese findings, in this first postmortem investigation of patients with ALS in CuATSM trials, demonstrate that in contrast to that seen in preclinical models of disease, CuATSM does not significantly alleviate neuronal pathology or astrogliosis in patients with ALS.
A phloem‐localized Arabidopsis metacaspase (AtMC3) improves drought tolerance
Authors: Pitsili Eugenia , Rodriguez‐Trevino Ricardo , Ruiz‐Solani Nerea , Demir Fatih , Kastanaki Elizabeth , Dambire Charlene , de Pedro‐Jové Roger , Vercammen Dominique , Salguero‐Linares Jose , Hall Hardy , Mantz Melissa , Schuler Martin , Tuominen Hannele , Van Breusegem Frank , Valls Marc , Munné‐Bosch Sergi , Holdsworth Michael J. , Huesgen Pitter F. , Rodriguez‐Villalon Antia , Coll Nuria S. 
Published: June 2023

Summary
Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in facilitating inter‐organ communication is critical and poorly understood.
Combining genetic, proteomic and physiological approaches, we investigated the role of AtMC3, a phloem‐specific member of the metacaspase family, in osmotic stress responses in Arabidopsis thaliana. Analyses of the proteome in plants with altered AtMC3 levels revealed differential abundance of proteins related to osmotic stress pointing into a role of the protein in water‐stress‐related responses.
Overexpression of AtMC3 conferred drought tolerance by enhancing the differentiation of specific vascular tissues and maintaining higher levels of vascular‐mediated transportation, while plants lacking the protein showed an impaired response to drought and inability to respond effectively to the hormone abscisic acid.
Overall, our data highlight the importance of AtMC3 and vascular plasticity in fine‐tuning early drought responses at the whole plant level without affecting growth or yield.
Synthesis and characterization of novel BODIPYs and their antioxidant, antimicrobial, photodynamic antimicrobial, antibiofilm and DNA interaction activities
Authors: Can Karanlık Ceren , Karanlık Gürkan , Özdemir Sadin , Tollu Gülşah , Erdoğmuş Ali 
Published: June 2023

AbstractIn the current study, we synthesized and characterized new BODIPY derivatives (1–4) having pyridine or thienyl‐pyridine substituents at meso‐ position and 4‐dibenzothienyl or benzo[b]thien‐2‐yl moieties at 2‐,6‐ positions. We investigated fluorescence properties and the ability to form singlet oxygen. In addition, various biological activities of BODIPYs such as DPPH scavenging, DNA binding/cleavage ability, cell viability inhibition, antimicrobial activity, antimicrobial photodynamic therapy (aPDT) and biofilm inhibition properties were performed. BODIPY derivatives BDPY‐3 (3) and BDPY‐4 (4) have high fluorescence quantum yields as 0.50 and 0.61 and 1O2 quantum yields were calculated as 0.83 for BDPY‐1 (1), 0.12 for BDPY‐2 (2), 0.11 for BDPY‐3 and 0.23 for BDPY‐4. BODIPY derivatives BDPY‐2, BDPY‐3 and BDPY‐4 displayed 92.54 ± 5.41%, 94.20 ± 5.50%, and 95.03 ± 5.54% antioxidant ability, respectively. BODIPY compounds showed excellent DNA chemical nuclease activity. BDPY‐2, BDPY‐3 and BDPY‐4 also exhibited 100% APDT activity against E. coli at all tested concentrations. In addition to these, they demonstrated a highly effective biofilm inhibition activity against Staphyloccous aureus and Pseudomans aeruginosa. BDPY‐4 showed the most effective antioxidant and DNA cleavage activity, while BDPY‐3 exhibited the most effective antimicrobial and antibiofilm activity.
Infusing theology in changemaking curricula: Engaging justice natives with Christian social thought
Authors: Bowman Jonathan M. 
Published: June 2023

AbstractAs “justice natives,” today's undergraduate students are motivated to engage directly with the world around them in ways unseen throughout most previous generations. Interestingly, secular humanistic perspectives on changemaking are in keeping with a wide variety of biblical teachings and principles of Christian social thought. At the same time, educators and practitioners can better engage these students to lessen a disconnect that often seems to emerge between social justice causes and the modern church. This paper applies a praxis‐based approach, Kingian Theology, the Beatitudes from the gospels, elements of liberation theology, Wesley's thoughts about community engagement, and principles of both Catholic and broader Christian social thought to the modern idea of changemaking, positioning Christianity and the gospels as a liberating force for equality rather than as a colonizing or even racist‐associated oppressive ideology.