bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes for active surveillance are similar for men with favourable risk ISUP-2 to those with ISUP-1 prostate cancer: A pair matched cohort study
Authors: Katelaris Athos , Amin Amer , Blazevski Alexandar , Scheltema Matthijs J , Cusick Thomas , Farraha Melad , Barreto Daniela , Haynes Anne Maree , Gondoputro William , Agrawal Shikha , Stricker Phillip , Thompson James 
Published: March 2023

Objective: To compare medium-term outcomes of active surveillance (AS) for men with favourable risk International Society for Urologic Pathology (ISUP)-2 prostate cancer (PCa) to a pair matched group of men with ISUP-1 PCa. Methods: This was a retrospective analysis of prospectively collected data from a single institution clinical outcomes registry, using propensity score matching. Men enrolled on AS with favourable risk ISUP-2 PCa with minimum 5-year follow-up were 1:2 propensity score matched to men with ISUP-1 disease. We assessed rates of progression to treatment, metastatic disease, adverse surgical pathology and overall survival. Results: Fifty-five ISUP-2 patients were matched to 105 ISUP-1 patients by propensity score. Median follow-up was 81 months (interquartile range (IQR), 61–109 months). Fifty-seven per cent in the ISUP-1 group progressed to treatment versus 58% in the ISUP-2 group (KM log rank p = 0.24). Estimated 1-, 2- and 5-year progression free survival rates were 93%, 60% and 33% for ISUP-1 patients and 94%, 63% and 16% for ISUP-2 patients, respectively. No patient from either group died of PCa. There was no statistical difference in rates of adverse pathology or metastatic disease between ISUP-2 and ISUP-1 patients on AS. Conclusion: AS for carefully selected men with favourable risk ISUP-2 disease appears safe, with similar oncologic outcomes to men with ISUP-1 disease. Level of evidence: Level 2b.
Comparative analysis of early post-operative outcomes between retzius-sparing and anterior approach robotic radical prostatectomy for a single surgeon
Authors: Tay Li June , Makin Robert , Saxionis Ioannis , Dokubo Ibi , Patel Keval , Sivathasan Sailantra , Smart Sonny , Warren Anne , Shah Nimish , Lamb Benjamin W 
Published: March 2023

Introduction: The aim of this study was to compare intraoperative and early post-operative outcomes between retzius-sparing robotic-assisted radical prostatectomy (RS-RARP) versus standard approach for RARP (S-RARP). Patients and methods: All RARPs by a single surgeon were included and divided into two groups: (1) standard approach including combined anterior–posterior approach (S-RARP); (2) retzius-sparing posterior approach (RS-RARP). Allocation was based on prostate size and location of index lesion on MRI. Initial post-operative follow-up was at 6 weeks. Results: Overall, 169 RARPs were performed between March 2018 and October 2021: S-RARP = 99 versus RS-RARP = 70. There was no significant difference in pre-operative body mass index (BMI), prostate-specific antigen (PSA), International Society of Urological Pathology (ISUP) grade group and clinical T stage. Intraoperative differences were found in blood loss (300 versus 200 mL, p = 0.008), console time (180 versus 135 minutes, p < 0.001) favouring RS-RARP, with no differences in nerve-spare or lymph node dissection. Post-operatively, no difference was found in ISUP grade, pathological T stage, positive surgical margins, number of lymph nodes sampled, readmissions or complications. Gland size in the RS group was smaller (38 versus 29 g, p = 0.001). Early (6 weeks) post-op follow-up showed a significant difference between groups for both pad-free continence (35% versus 53%, p = 0.011) and social continence (79% versus 89%, p = 0.024), but no difference for erectile function recovery (27% versus 50% of baseline) and post-op PSA levels < 0.1 ng/mL (85% versus 93%). Conclusion: Even early in the learning curve, continence recovery, operative time and blood loss were significantly better for RS-RARP than S-RARP. Margin status and PSA levels are comparable to published literature for both groups. Standardised training in RS-RARP might help to improve the uptake of this novel technique. Level of evidence: 2.
Network analysis of internet gaming disorder, problematic social media use, problematic smartphone use, psychological distress, and meaning in life among adolescents
Authors: Li Yumei , Mu Wenlong , Xie Xuying , Kwok Sylvia Y.C.L. 
Published: March 2023

Background Few studies have examined the connections between psychological distress, meaning in life, internet gaming disorder (IGD), problematic smartphone use (PSU), and problematic social media use (PSMU). Methods The central and bridging nodes of IGD, PSMU, and PSU symptoms were investigated using network analysis in China among 742 adolescents ( Mage = 15.39, SD = 1.68, range = 12–19; 53.23% female). The relationships between IGD, PSMU, and PSU and associated factors were investigated using a Directed Acyclic Graphs method. Results The results suggested that the central symptoms were withdrawal in IGD, mood modification in PSU, and tolerance in PSMU. Mood modification in IGD, mood modification in PSU, withdrawal in PSMU, and functional impairment in PSMU were the bridge symptoms. Males were more likely to experience symptoms of IGD than females, while females were more likely to need meaning than males. Stress is the root factor, while depression, meaning confusion, meaning anxiety, and meaning avoidance were closely associated with IGD, PSMU, and PSU. Conclusions The current research improved the understanding of IGD, PSMU, and PSU symptoms in teenagers and demonstrated the potential of dynamic systems perspectives on problematic use behaviors and stress/meaning-focused interventions.
Applicable of teledentistry and digital platforms during the COVID-19 pandemic among dentists in Fiji: A qualitative study
Authors: Kajal Kartika , Mohammadnezhad Masoud 
Published: March 2023

Objectives This research aims to explore the perception of dental officers (DOs) and dental managers (DMs) on the use of teledentistry and digital platforms during the COVID-19 pandemic in Fiji. Methods This qualitative study was conducted among 30 DOs and 17 DMs. The study was conducted in private dental clinics, government dental clinics and the School of Dentistry and Oral Health clinic (SDOH), in the Central Division, Fiji that were selected randomly. The participants were selected using the purposive sampling method using the inclusion and exclusion criteria. Data was collected through in-depth interviews via zoom using a self-developed semi-structure open-ended interview questionnaire. Manual thematic analysis of the data was conducted. Results Five themes emerged from data analysis: utilization of teledentistry and media during the pandemic, usefulness of teledentistry, confidence with teledentistry and digital platforms-associated risks, digital platforms for record keeping, and teledentistry promotion and training. Teledentistry was mostly utilized by private practitioners and the oral surgery department. Practitioners were not in favour of teledentistry as they thought it was putting them at risk. Conclusion Majority of DOs and DMs were not utilizing teledentistry except for a few private dental practitioners and the oral surgery department. Future research can be conducted in other divisions and include other healthcare professionals.
The Effect of Hypertension and Diabetes on Ophthalmic Artery Hemodynamics
Authors: Fatima Hamnah , Asad Naveed , Madina Syeda Rabi , Malik Sajid Shaheen , Hanif Asif 
Published: March 2023

Objective: Hypertensive and diabetic patients are at higher risk of developing ocular complications. Color and spectral Doppler, as a noninvasive technique, can be used to monitor and access the ophthalmic artery (OA) and its hemodynamic characteristics. The aim of this study was to investigate the hemodynamics of OA in diabetes and hypertension. Materials and Methods: This cross-sectional study was conducted at the University of Lahore sonography clinic. The patients were investigated using Toshiba Xario with 5 to 7.5 MHz linear transducer and the scanning was completed with closed eyelids. Those included in the study were hypertensive and diabetic patients. Exclusions were made based on a patient’s history of eye surgeries, trauma, inflammation, glaucoma, cataract, and pregnancy. Results: There were 200 patients recruited and of which, 41 were hypertensive and diabetic, whereas 159 were normal subjects. In this cohort, 41 patients (20%) were positive for hypertension and diabetes. There were 159 patients (79%) were negative for diabetes and hypertension. The resistive index (RI) of right OA was 0.783 ± 0.114 with 0.029. When considering the OA hemodynamics recorded, the pulsatility index (PI), the systolic/diastolic (S/D) ratio, RI, and end diastolic velocity (EDV) were significantly correlated among those with hypertension and diabetes. In the normal subjects, all hemodynamic measurements were seen as normal, when compared to those with hypertension and diabetic. Conclusion: This cohort study may suggest that there is a pathological increase in vascular resistance, PI, S/D ratio, and decreased blood flow velocities, in ophthalmic artery, for those hypertensive and diabetic patients.
HLH-like toxicities predict poor survival after the use of tisagenlecleucel in children and young adults with B-ALL
Authors: McNerney Kevin O. , Si Lim Stephanie J. , Ishikawa Kyle , Dreyzin Alexandra , Vatsayan Anant , Chen John J. , Baggott Christina , Prabhu Snehit , Pacenta Holly L. , Philips Christine , Rossoff Jenna , Stefanski Heather E. , Talano Julie-An , Moskop Amy , Verneris Michael , Myers Doug , Karras Nicole A. , Brown Patrick , Bonifant Challice L. , Qayed Muna , Hermiston Michelle , Satwani Prakash , Krupski Christa , Keating Amy K. , Baumeister Susanne H. C. , Fabrizio Vanessa A. , Chinnabhandar Vasant , Egeler Emily , Mavroukakis Sharon , Curran Kevin J. , Mackall Crystal L. , Laetsch Theodore W. , Schultz Liora M. 
Published: March 2023

Abstract
Chimeric antigen receptor–associated hemophagocytic lymphohistiocytosis (HLH)–like toxicities (LTs) involving hyperferritinemia, multiorgan dysfunction, coagulopathy, and/or hemophagocytosis are described as occurring in a subset of patients with cytokine release syndrome (CRS). Case series report poor outcomes for those with B-cell acute lymphoblastic leukemia (B-ALL) who develop HLH-LTs, although larger outcomes analyses of children and young adults (CAYAs) with B-ALL who develop these toxicities after the administration of commercially available tisagenlecleucel are not described. Using a multi-institutional database of 185 CAYAs with B-ALL, we conducted a retrospective cohort study including groups that developed HLH-LTs, high-grade (HG) CRS without HLH-LTs, or no to low-grade (NLG) CRS without HLH-LTs. Primary objectives included characterizing the incidence, outcomes, and preinfusion factors associated with HLH-LTs. Among 185 CAYAs infused with tisagenlecleucel, 26 (14.1%) met the criteria for HLH-LTs. One-year overall survival and relapse-free survival were 25.7% and 4.7%, respectively, in those with HLH-LTs compared with 80.1% and 57.6%, respectively, in those without. In multivariable analysis for death, meeting criteria for HLH-LTs carried a hazard ratio of 4.61 (95% confidence interval, 2.41-8.83), controlling for disease burden, age, and sex. Patients who developed HLH-LTs had higher pretisagenlecleucel disease burden, ferritin, and C-reactive protein levels and lower platelet and absolute neutrophil counts than patients with HG- or NLG-CRS without HLH-LTs. Overall, CAYAs with B-ALL who developed HLH-LTs after tisagenlecleucel experienced high rates of relapse and nonrelapse mortality, indicating the urgent need for further investigations into prevention and optimal management of patients who develop HLH-LTs after tisagenlecleucel.
Risk of infections associated with the use of bispecific antibodies in multiple myeloma: a pooled analysis
Authors: Mazahreh Farah , Mazahreh Liyan , Schinke Carolina , Thanendrarajan Sharmilan , Zangari Maurizio , Shaughnessy John D. , Zhan Fenghuang , van Rhee Frits , Al Hadidi Samer 
Published: March 2023

Abstract
The use of bispecific antibodies (BsAbs) in the treatment of relapsed/refractory multiple myeloma (MM) is showing early promising overall response rates in heavily pretreated patients. Infectious complications related to the use of BsAbs are not well described. We conducted a pooled analysis that included all single-agent BsAbs used in MM with no prior use of different BsAbs. A total of 1185 patients with MM were treated with a BsAb in the studied period (71.6% of the patients treated with an agent targeting B-cell maturation antigen (BCMA). Pooled median follow-up was short at 6.1 months (7.5 vs 5.2 months for BCMA vs non-BCMA BsAbs, respectively). Adverse events of interest included all grade neutropenia in 38.6%, all grade infections in 50% (n = 542/1083), all grade cytokine release syndrome in 59.6% (n = 706/1185), grade III/IV neutropenia in 34.8% (n = 372/1068), grade III/IV infections in 24.5% (n = 272/1110), grade III/IV pneumonia in 10% (n = 52.4/506), and grade III/IV coronavirus disease 2019 in 11.4% (n = 45.4/395) of the patients. Non-BCMA–targeted BsAbs were associated with lower grade III/IV neutropenia (25.3% vs 39.2%) and lower grade III/IV infections (11.9% vs 30%) when compared with BCMA-targeted BsAbs. Hypogammaglobulinemia was reported in 4 studies, with a prevalence of 75.3% (n = 256/340) of the patients, with IV immunoglobulin used in 48% (n = 123/256) of them. Death was reported in 110 patients, of which 28 (25.5%) were reported to be secondary to infections. Certain precautions should be used when using BsAbs to mitigate the risk and/or identify and treat infections promptly.
Correlative analysis on InGaN/GaN nanowires: structural and optical properties of self-assembled short-period superlattices
Authors: Alonso-Orts Manuel , Hötzel Rudolfo , Grieb Tim , Auf der Maur Matthias , Ries Maximilian , Nippert Felix , März Benjamin , Müller-Caspary Knut , Wagner Markus R. , Rosenauer Andreas , Eickhoff Martin 
Published: March 2023

AbstractThe influence of self-assembled short-period superlattices (SPSLs) on the structural and optical properties of InGaN/GaN nanowires (NWs) grown by PAMBE on Si (111) was investigated by STEM, EDXS, µ-PL analysis and k·p simulations. STEM analysis on single NWs indicates that in most of the studied nanostructures, SPSLs self-assemble during growth. The SPSLs display short-range ordering of In-rich and In-poor InxGa1-xN regions with a period of 2–3 nm that are covered by a GaN shell and that transition to a more homogenous InxGa1-xN core. Polarization- and temperature-resolved PL analysis performed on the same NWs shows that they exhibit a strong parallel polarized red-yellow emission and a predominantly perpendicular polarized blue emission, which are ascribed to different In-rich regions in the nanostructures. The correlation between STEM, µ-PL and k·p simulations provides better understanding of the rich optical emission of complex III-N nanostructures and how they are impacted by structural properties, yielding the significant impact of strain on self-assembly and spectral emission.
Graphical abstract
Diagnosis and treatment approaches for simultaneous onset of subarachnoid hemorrhage and thyroid storm: a case report
Authors: Ohya Aimi , Ohtake Makoto , Kawamura Yusuke , Akimoto Taisuke , Iwashita Masayuki , Yamamoto Tetsuya , Takeuchi Ichiro 
Published: March 2023

AbstractBackgroundSubarachnoid hemorrhage and thyroid storm are similar in their clinical symptomatology, and diagnosis of these conditions, when they occur simultaneously, is difficult. Here, we report a rare case of concurrent subarachnoid hemorrhage and thyroid storm we encountered at our hospital.Case presentationThe patient was a 52-year-old woman. While bathing at home, the patient experienced a sudden disturbance of consciousness and was brought to our hospital. The main physical findings upon admittance were Glasgow Coma Scale score of E1V2M4, elevated blood pressure (208/145 mmHg), and tachycardia with atrial fibrillation (180 bpm) along with body temperature of 36.1 °C. Brain computed tomography revealed subarachnoid hemorrhage associated with a ruptured aneurysm of the posterior communicating artery branching from the left internal carotid artery, and aneurysm clipping was performed. Blood tests upon admission revealed high levels of free T3 and free T4 and low levels of thyroid-stimulating hormone. Upon determining that the patient had hyperthyroidism, thiamazole was administered. However, due to continuous impaired consciousness, fever, and persistence of tachycardia, the patient was diagnosed with thyroid storm. Oral potassium iodide and hydrocortisone were added to the treatment. The treatment was successful as the patient’s symptoms improved, and she became lucid.In this case, we believe that in the presence of untreated hyperthyroidism, the onset of subarachnoid hemorrhage induced thyroid storm. Tachycardia of 130 bpm or higher, which is the diagnostic criterion for thyroid storm, rarely occurs with subarachnoid hemorrhage. Therefore, we believe it is an important factor for recognizing the presence of the thyroid storm. In this case, clipping surgery was prioritized which resulted in a favorable outcome. However, it is possible that invasive surgery may have exacerbated thyroid storm, suggesting that treatment should be tailored as per patient’s condition.ConclusionIf a pulse rate of 130 bpm or higher is observed alongside subarachnoid hemorrhage, we recommend considering the possibility of concomitant thyroid storm and testing for thyroid hormone. If concomitant thyroid storm is present, we believe that a treatment plan tailored to the patient’s condition is critical, and early diagnosis will lead to a favorable outcome for the patient.
DeltaMSI: artificial intelligence-based modeling of microsatellite instability scoring on next-generation sequencing data
Authors: Swaerts Koen , Dedeurwaerdere Franceska , De Smet Dieter , De Jaeger Peter , Martens Geert A. 
Published: March 2023

AbstractBackgroundDNA mismatch repair deficiency (dMMR) testing is crucial for detection of microsatellite unstable (MSI) tumors. MSI is detected by aberrant indel length distributions of microsatellite markers, either by visual inspection of PCR-fragment length profiles or by automated bioinformatic scoring on next-generation sequencing (NGS) data. The former is time-consuming and low-throughput while the latter typically relies on simplified binary scoring of a single parameter of the indel distribution. The purpose of this study was to use machine learning to process the full complexity of indel distributions and integrate it into a robust script for screening of dMMR on small gene panel-based NGS data of clinical tumor samples without paired normal tissue.MethodsScikit-learn was used to train 7 models on normalized read depth data of 36 microsatellite loci in a cohort of 133 MMR proficient (pMMR) and 46 dMMR tumor samples, taking loss of MLH1/MSH2/PMS2/MSH6 protein expression as reference method. After selection of the optimal model and microsatellite panel the two top-performing models per locus (logistic regression and support vector machine) were integrated into a novel script (DeltaMSI) for combined prediction of MSI status on 28 marker loci at sample level. Diagnostic performance of DeltaMSI was compared to that of mSINGS, a widely used script for MSI detection on unpaired tumor samples. The robustness of DeltaMSI was evaluated on 1072 unselected, consecutive solid tumor samples in a real-world setting sequenced using capture chemistry, and 116 solid tumor samples sequenced by amplicon chemistry. Likelihood ratios were used to select result intervals with clinical validity.ResultsDeltaMSI achieved higher robustness at equal diagnostic power (AUC = 0.950; 95% CI 0.910–0.975) as compared to mSINGS (AUC = 0.876; 95% CI 0.823–0.918). Its sensitivity of 90% at 100% specificity indicated its clinical potential for high-throughput MSI screening in all tumor types.Clinical Trial Number/IRBB1172020000040, Ethical Committee, AZ Delta General Hospital.
Amino acid substitutions in specific proteins correlate with farnesol unresponsiveness in Candida albicans
Authors: Mohammadi Sima , Leduc Annie , Charette Steve J. , Barbeau Jean , Vincent Antony T. 
Published: March 2023

Abstract
Background
The quorum-sensing molecule farnesol, in opportunistic yeast Candida albicans, modulates its dimorphic switch between yeast and hyphal forms, and biofilm formation. Although there is an increasing interest in farnesol as a potential antifungal drug, the molecular mechanism by which C. albicans responds to this molecule is still not fully understood.

Results
A comparative genomic analysis between C. albicans strains that are naturally unresponsive to 30 µM of farnesol on TYE plates at 37 °C versus responsive strains uncovered new molecular determinants involved in the response to farnesol. While no signature gene was identified, amino acid changes in specific proteins were shown to correlate with the unresponsiveness to farnesol, particularly with substitutions in proteins known to be involved in the farnesol response. Although amino acid changes occur primarily in disordered regions of proteins, some amino acid changes were also found in known domains. Finally, the genomic investigation of intermediate-response strains showed that the non-response to farnesol occurs gradually following the successive accumulation of amino acid changes at specific positions.

Conclusion
It is known that large genomic changes, such as recombinations and gene flow (losses and gains), can cause major phenotypic changes in pathogens. However, it is still not well known or documented how more subtle changes, such as amino acid substitutions, play a role in the adaptation of pathogens. The present study shows that amino acid changes can modulate C. albicans yeast’s response to farnesol. This study also improves our understanding of the network of proteins involved in the response to farnesol, and of the involvement of amino acid substitutions in cellular behavior.