bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global identification of mammalian host and nested gene pairs reveal tissue-specific transcriptional interplay
Authors: Montibus Bertille , Cain James , Martinez-Nunez Rocio T , Oakey Rebecca J. 
Published: May 2023

ABSTRACTNucleotide sequences along a gene provide instructions to transcriptional and co-transcriptional machinery allowing genome expansion into the transcriptome. Interestingly, nucleotide sequence can often be shared between two genes and in some occurrences, a gene is located completely within a different gene, these are known as host/nested genes pairs. In these instances, if both genes are transcribed, overlap can result in a transcriptional crosstalk where genes regulate each other. Despite this, a comprehensive annotation of where such genes are located, and their expression patterns is lacking. To address this, we provide an up-to-date catalogue of host/nested gene pairs in mouse and human, showing that over a tenth of all genes contain a nested gene. We discovered that transcriptional co-occurrence is often tissue-specific. This co-expression was especially prevalent within the transcriptionally permissive tissue, testis. We used this developmental system and scRNA-seq analysis to demonstrate that co-expression of pairs can occur in single cells and transcription in the same place at the same time can enhance transcript diversity of the host gene. In agreement, host genes are more transcript diverse than the rest of the transcriptome and we propose that nested gene expression drives this observed diversity. Given that host/nested gene configurations were common in both human and mouse genomes, the interplay between pairs is therefore likely selected for, highlighting the relevance of transcriptional crosstalk between genes which share nucleic acid sequence. The results and analysis are available on an Rshiny application.
Targeting APEX2 to the mRNA encoding fatty acid synthase β in yeast identifies proteins that bind and control its translational efficiency in the cell cycle
Authors: Blank Heidi M. , Griffith Wendell P. , Polymenis Michael 
Published: May 2023

ABSTRACTProfiling the repertoire of proteins associated with a given mRNA during the cell cycle is unstudied. Furthermore, it is much easier to ask and answer what mRNAs a specific protein might bind to than the other way around. Here, we implemented an RNA-centric proximity labeling technology at different points in the cell cycle in highly synchronous yeast cultures. To understand how the translation ofFAS1, encoding fatty acid synthase, peaks late in the cell cycle, we identified proteins that bind theFAS1transcript in a cell cycle-dependent manner. We used dCas13d-APEX2 fusions to targetFAS1and label nearby proteins, which were then identified by mass spectrometry. The glycolytic enzyme Tdh3p, a known RNA-binding protein, bound theFAS1mRNA, and it was necessary for the increased Fas1p expression late in the cell cycle. Lastly, cells lacking Tdh3p had altered size homeostasis, consistent with delayed G1/S transition and exit from mitosis. These results point to unexpected connections between major metabolic pathways. They also underscore the role of mRNA-protein interactions for gene expression during cell division.
Evidence for the Type IV Pili Retraction Motor PilT as a Component of the Surface Sensing System inPseudomonas aeruginosa
Authors: Geiger C.J. , O’Toole G.A. 
Published: May 2023

AbstractBiofilm formation begins when bacteria contacting a surface induce cellular changes to become better adapted for surface growth. One of the first changes to occur forPseudomonas aeruginosaafter surface contact is an increase in the nucleotide second messenger 3’,5’-cyclic adenosine monophosphate (cAMP). It has been demonstrated that this increase in intracellular cAMP is dependent on functional Type IV pili (T4P) relaying a signal to the Pil-Chp system, but the mechanism by which this signal is transduced remains poorly understood. Here, we investigate the role of the Type IV pili retraction motor PilT in sensing a surface and relaying that signal to cAMP production. We show that mutations affecting the structure of PilT and in particular ATPase activity of this motor protein, reduce surface-dependent cAMP production. We identify a novel interaction between PilT and PilJ, a member of the Pil-Chp system, and propose a new model wherebyP. aeruginosauses its retraction motor to sense a surface and to relay that signal via PilJ to increased production of cAMP. We discuss these findings in light of current TFP-dependent surface sensing models forP. aeruginosa.ImportanceT4P are cellular appendages that allowP. aeruginosato sense a surface leading to the production of cAMP. This second messenger not only activates virulence pathways but leads to further surface adaptation and irreversible attachment of cells. Here, we demonstrate the importance of the retraction motor PilT in surface sensing. We also present a new surface sensing model inP. aeruginosawhereby the T4P retraction motor PilT senses and transmits the surface signal, likely via its ATPase domain and interaction with PilJ, to mediate production of the second messenger cAMP.
DNA CombingversusDNA Spreading and the Separation of Sister Chromatids
Authors: Meroni Alice , Wells Sophie E. , Fonseca Carmen , Ray Chaudhuri Arnab , Caldecott Keith W. , Vindigni Alessandro 
Published: May 2023

SummaryDNA combing and DNA spreading are two central approaches for studying DNA replication fork dynamics genome-wide at single-molecule resolution by distributing labeled genomic DNA on coverslips or slides for immunodetection. Perturbations in DNA replication fork dynamics can differentially affect either leading or lagging strand synthesis, for example in instances where replication is blocked by a lesion or obstacle on only one of the two strands. Thus, we sought to investigate whether the DNA combing and/or spreading approaches are suitable for resolving adjacent sister chromatids during DNA replication, thereby enabling the detection of DNA replication dynamics within individual nascent strands. To this end, we developed a thymidine labeling scheme that discriminates between these two possibilities. Our data suggests that DNA combing resolves single chromatids, allowing the detection of strand-specific alterations, whereas DNA spreading does not. These findings have important implications when interpreting DNA replication dynamics from data obtained by these two commonly used techniques.
Adoption of electronic commerce as a resilience strategy for women's entrepreneurship in the Democratic Republic of Congo
Authors: Kaningini Euphrasie Wamunzila , Malinga Christine Mwati , Furaha Germaine Mirindi , Alulea Jonathan Pembwe , Castiaux Annick 
Published: May 2023

PurposeThe present article aims to determine the factors that explain the intention to adopt electronic commerce among women traders in a developing country like Democratic Republic of Congo (DRC) during a health crisis period.Design/methodology/approachThis study was conducted in the DRC, in Bukavu Town. A convenience sample of 282 respondents consisting of solely women entrepreneurs (importing traders) in Bukavu Town was selected and the structural equation model was used to test the research hypotheses resulted from Ajzen's theory of planned behaviour.FindingsThe finding results showed that only the factors attitude towards electronic commerce adoption and subjective norms which predict women traders' intention to adopt electronic commerce. The analysis shows that about 38.9% of the variation in the dependent variable is explained by the above variables.Originality/valueFew studies have presented technology and electronic commerce adoption as resilience of women entrepreneurs in a time of crisis, despite the abundance of the review literature on adoption. This study provides a new approach to assist women entrepreneurs as well as researchers in understanding the drivers of electronic commerce adoption factors in the DRC.
The effect of robot anthropomorphism on revisit intentions after service failure: a moderated serial mediation model
Authors: Cui Jinsheng , Zhong Jianan 
Published: May 2023

PurposeThe purpose of this study is to examine how robotic anthropomorphism and personalized design may affect consumers' reactions to brands after service failure.Design/methodology/approachThis study conducted two studies based on cognitive appraisal theory and artificial intelligence device acceptance theory. Study 1 explored the mechanisms by which the type of anthropomorphic design of the service robot (humanoid robot/nonhumanoid robot) influenced revisit intention after service failure through a one-factor between-subjects design based on a restaurant dining scenario. Study 2 was based on a hotel check-in scenario and explored the moderating effect of robot personalization design on the above mechanisms through a 2 (anthropomorphic design: humanoid robot/nonhumanoid robot) × 2 (personalized design: self-name/no name) between-subjects design.FindingsStudy 1 shows that consumers have higher performance expectations for nonhumanoid robots, leading to a higher tolerance for service failure, which in turn generates higher revisit intentions. Study 2 shows that consumers' performance expectations are significantly enhanced after custom naming of humanoid robots, so the serial mediation mechanism for the effect of robot anthropomorphic design on revisit intention does not hold.Originality/valueThis study extends the research of artificial intelligence device acceptance theory in the field of service failure and exploratively proposes an intervention mechanism for the negative effects of the anthropomorphic design of service robots.
The blame game: disaster, queerness and prejudice
Authors: Rushton Ashleigh , Scarlett Jazmin 
Published: May 2023

PurposeThe purpose of this article is to draw attention to how harmful and inaccurate discourses pertaining to disaster responsibility is produced, the negative implications such narratives pose and the role of the media in the ways in which discourses about queerness and disaster are reported.Design/methodology/approachThroughout this paper, the authors detail examples of media reporting on discourses relating to people with diverse sexual orientation, gender identity, gender expression and sex characteristics (SOGIESC) being blamed and held responsible for disasters across the world. The authors examine the value of such reporting as well as describing the harm blame narratives have on queer people and communities.FindingsThere is little value in reporting on accounts of people publicly declaring that people with diverse SOGIESC are to blame for disaster. More sensitivity is needed around publishing on blame discourses pertaining to already marginalised communities.Originality/valueThis article contributes to the developing scholarship on lesbian, gay, bisexual, transgender, queer, intersex, agender, asexual and aromantic individuals, plus other gender identities and sexual orientations (LGBTQIA+/SOGIESC) and disasters by detailing the harm of blame discourses as well as drawing attention to how the media have a role to play in averting from unintentionally providing a platform for hate speech and ultimately enhancing prejudice against people with diverse SOGIESC.
Cryptocurrency estate planning: the challenges, suggested solutions and Malaysia’s future directions
Authors: Katuk Norliza , Abd Wahab Norazlina , Kamis Nur Syaedah 
Published: May 2023

PurposeThis paper aims to collect evidence on the issues and challenges in cryptocurrency estate planning and the possible approaches or methods to address them. Then, this paper also aims to understand Malaysia’s situation compared to global situations and determine how the country could address those challenges.Design/methodology/approachThis study adapted a standard systematic review protocol named preferred reporting items for systematic reviews and meta-analyses, guided by three research questions: what are the challenges of cryptocurrency estate planning, how do researchers and practitioners address the issues or challenges and how could Malaysians move towards sustainable cryptocurrency estate planning? Relevant documents reported on cryptocurrency estate planning were analysed using thematic analysis.FindingsThe results of this review suggested that the issues and challenges in cryptocurrency estate planning can be described from six aspects: awareness, financial aspects, law, process, security and taxation. On the other hand, the approaches or methods to address these challenges may be categorised into laws, processes and technology. Further, a framework for cryptocurrency estate planning in Malaysia is proposed as an initial step towards a sustainable economy and society in Malaysia.Research limitations/implicationsThe state and the use of cryptocurrency are still considered new in most countries globally. Hence, people are yet to be aware of the issues that may arise from it. Nevertheless, cryptocurrency estate planning will soon become an area of interest in which this study could enhance the literature and contribute knowledge to understanding the situation.Originality/valueThis study collected evidence on the issues and the approaches to address cryptocurrency estate planning. This study then focused on the Malaysian perspective by proposing a framework that was not covered by existing studies.
Communication apprehension and self-perceived communication competence: a study of undergraduate students in their final year
Authors: Muftah Muneera 
Published: May 2023

PurposeCommunication apprehension (CA) is the fear or anxiety about communicating. In addition, self-perceived communication competence (SPCC) refers to the learners' perception of their competence. CA hampers individuals' ability to communicate effectively in various communication contexts, which has an impact on their employability. Confident communicators with effective communication skills are important issues for graduation as well as desirable attributes for recruiting agencies. Given that employers value graduates who can communicate effectively in the workplace without any apprehension, this study was designed to determine the levels of CA and SPCC among final-year non-English major undergraduate students in Saudi Arabia and the extent to which SPCC could affect their CA when speaking in English with implications for their employability as the next generation of workers.Design/methodology/approachTwo survey questionnaires were administered to 70 non-English major undergraduate students in their final year employing probability random sampling to measure their CA level and to assess their SPCC in different English as foreign language (EFL) contexts. Pearson's correlation and linear regression analysis were used to investigate the relationship between CA and SPCC. Data analysis was performed using descriptive statistics in SPSS 21.FindingsThe findings indicated a medium level of SPCC and CA. Moreover, the correlation formula revealed that there was a moderately significant association between learners' SPCC and their CA (r (70) = −0.524, p < 0.01). Through regression analysis, it was found that their SPCC could predict 27% of the variance in their CA. The moderate effect of SPCC on CA implied that students could be prepared to cope and perform in the workplace.Originality/valueWithout communication, the establishment and evolution of the human community are unattainable. This aspect is more evident and crucial in the list of graduate objectives and needed outcomes for future career endeavors. The findings encourage instructors and course designers to provide many learning opportunities in pleasurable learning environments to raise students' perceptions of their competence, in turn enhancing their confidence to speak in English and ensuring a smooth transition from academia to the workplace. Based on the findings, several suggestions for future studies in the EFL context are presented.
Determining psycholinguistic features of deception in phishing messages
Authors: Xu Tianhao , Rajivan Prashanth 
Published: May 2023

PurposeDistinguishing phishing emails from legitimate emails continues to be a difficult task for most individuals. This study aims to investigate the psycholinguistic factors associated with deception in phishing email text and their effect on end-user ability to discriminate phishing emails from legitimate emails.Design/methodology/approachEmail messages and end-user decisions collected from a laboratory phishing study were validated and analyzed using natural language processing methods (Linguistic Inquiry Word Count) and penalized regression models (LASSO and Elastic Net) to determine the linguistic dimensions that attackers may use in phishing emails to deceive end-users and measure the impact of such choices on end-user susceptibility to phishing.FindingsWe found that most participants, who played the role of a phisher in the study, chose to deceive their end-user targets by pretending to be a familiar individual and presenting time pressure or deadlines. Results show that use of words conveying certainty (e.g. always, never) and work-related features in the phishing messages predicted higher end-user vulnerability. On the contrary, use of words that convey achievement (e.g. earn, win) or reward (cash, money) in the phishing messages predicted lower end-user vulnerability because such features are usually observed in scam-like messages.Practical implicationsInsights from this research show that analyzing emails for psycholinguistic features associated with computer-mediated deception could be used to fine-tune and improve spam and phishing detection technologies. This research also informs the kinds of phishing attacks that must be prioritized in antiphishing training programs.Originality/valueApplying natural language processing and statistical modeling methods to analyze results from a laboratory phishing experiment to understand deception from both attacker and end-user is novel. Furthermore, results from this work advance our understanding of the linguistic factors associated with deception in phishing email text and its impact on end-user susceptibility.
Financial well-being, family financial support and depression of older adults in China
Authors: Fan Lu , Lei Shan 
Published: May 2023

PurposeThis study aims to examine the relationship between objective and subjective aspects of financial well-being, the role of family financial support and depression symptoms of Chinese older adults.Design/methodology/approachThis study used two waves (2015 and 2018) of the Harmonized China Health and Retirement Longitudinal Study. Two financial ratios: the expenditure-to-income ratio and the financial assets ratio, were used to measure the objective aspect of financial well-being. Perceived money management difficulty was employed to measure the subjective aspect of financial well-being. Depression symptoms were measured using the Center for Epidemiologic Studies Depression Scale (CES-D) score. Three analytical models, including an ordinary least squares (OLS) model, an OLS model controlling for lagged depression and a random effects model using panel data, were used to examine the relationships between the objective and subject aspects of financial well-being and depression.FindingsThe results from the three models showed consistent relationships: the expenditure-to-income ratio was a positive contributor, while the financial assets ratio was a negative contributor to depression of older adults in China. The robustness check using binary-coded financial ratio thresholds showed that reaching the suggested thresholds was negatively associated with depression. Perceived money management difficulty contributed positively to depression. The robustness check using the fixed effects model showed no significance of the two ratios, while perceived money management difficulty was positively associated with depression. The insignificance might be due to data limitation (limited waves or rare changes across waves).Originality/valueThe findings indicate that both objective and subjective financial well-being matters in relation to depression symptoms and, therefore, to the overall mental health of the Chinese elderly. Developments in public policies are needed to promote accessible financial services, assistance programs, mental health services and facilities for the older population in China.
COVID-19 pandemic disruptions and environmental turbulence in architectural, engineering and construction project delivery space
Authors: Adesi Michael , Ewuga Duga , Owusu-Manu De-Graft , Boateng Frank , Kissi Ernest 
Published: May 2023

PurposeFirms in the architectural, engineering, construction and operations (AECO) sector continue undertaking projects in a disruptive environment due to the coronavirus disease 2019 (COVID-19) pandemic. This study aims to explore environmental turbulence in the AECO project delivery space and suggest mechanisms for enhancing resilience against future pandemics.Design/methodology/approachThe study adopts the quantitative approach by administering 110 survey questionnaires to participants comprising project managers, site engineers, quantity surveyors, contractors and subcontractors.FindingsThe study identifies 24 COVID-19 disruptions linked to environmental turbulence categorised as scheduling, performance and productivity, project budget, supply chain, resource allocation and technological and regulatory. The study suggested resilient mechanisms for surviving in future pandemics.Originality/valueThis study enhances the understanding of environmental turbulence from the perspective of COVID-19 disruptions in AECO project delivery, while the implementation of the resilient mechanisms improves capability of AECO firms against future pandemics.
An integrated path framework of tourism and hospitality social entrepreneurship: a systematic literature review
Authors: Zhang Yan , Xu Hong , Yang Hongyan 
Published: May 2023

Purpose
As an important emerging topic in recent years, reviews on tourism and hospitality social entrepreneurship (THSE) by theme remain limited. This paper aims to map the current publication trends, reveal the formation path and identify the future research directions of THSE, providing comprehensive and in-depth insight.

Design/methodology/approach
This study presents a systematic review of 89 articles on THSE published in the Web of Science, ScienceDirect and EBSCOhost. It follows grounded theory to classify literature and an expert feedback process to ensure validity.

Findings
Research on THSE is mainly found in developing countries and regions. Qualitative methods have been the most popular for researchers. This paper classifies THSE research into four main themes: antecedents, processes, effects and challenges.

Practical implications
This review suggests that social enterprise managers should consider the inclusive business model and adopt empowerment, network construction, market approaches and bricolage to mobilize resources. Policymakers should disseminate relevant laws and regulations to provide a flexible and equal environment. Education and capacity building for people should be strengthened to train future social entrepreneurs.

Originality/value
This review provides an integrated process model by clustering articles to update the holistic picture of THSE. This work identifies research gaps that future research should address.