bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitroformation and extended culture of highly metabolically active and contractile tissues
Authors: Bagdasarian Isabella A. , Tonmoy Thamidul Islam , Park B. Hyle , Morgan Joshua T. 
Published: July 2023

Abstract3D cell culture models have gained popularity in recent years as an alternative to animal and 2D cell culture models for pharmaceutical testing and disease modeling. Polydimethylsiloxane (PDMS) is a cost-effective and accessible molding material for 3D cultures; however, routine PDMS molding may not be appropriate for extended culture of contractile and metabolically active tissues. Failures can include loss of culture adhesion to the PDMS mold and limited culture surfaces for nutrient and waste diffusion. In this study, we evaluated PDMS molding materials and surface treatments for highly contractile and metabolically active 3D cell cultures. PDMS functionalized with polydopamine allowed for extended culture duration (14.8 ± 3.97 days) when compared to polyethylamine/glutaraldehyde functionalization (6.94 ± 2.74 days); Additionally, porous PDMS extended culture duration (16.7 ± 3.51 days) compared to smooth PDMS (6.33 ± 2.05 days) after treatment with TGF-β2 to increase culture contraction. Porous PDMS additionally allowed for large (13 mm tall × 8 mm diameter) constructs to be fed by diffusion through the mold, resulting in increased cell density (0.0210 ± 0.0049 mean nuclear fraction) compared to controls (0.0045 ± 0.0016 mean nuclear fraction). As a practical demonstration of the flexibility of porous PDMS, we engineered a vascular bioartificial muscle model (VBAM) and demonstrated extended culture of VBAMs anchored with porous PDMS posts. Using this model, we assessed the effect of feeding frequency on VBAM cellularity. Feeding 3×/week significantly increased nuclear fraction at multiple tissue depths relative to 2×/day. VBAM maturation was similarly improved in 3×/week feeding as measured by nuclear alignment (23.49° ± 3.644) and nuclear aspect ratio (2.274 ± 0.0643) relative to 2x/day (35.93° ± 2.942) and (1.371 ± 0.1127), respectively. The described techniques are designed to be simple and easy to implement with minimal training or expense, improving access to dense and/or metabolically active 3D cell culture models.
A typology of user misbehaviours in the sharing economy context
Authors: Golf-Papez Maja , Culiberg Barbara 
Published: July 2023

Purpose
This paper aims to examine the types of user misbehaviours in the sharing economy (SE) context. SE offers a fruitful study setting due to the scope of potential misbehaviour and the expanded role of consumers.

Design/methodology/approach
The study drew on online archival data from the AirbnbHell.com website, where people share their stories about their Airbnb-related negative experiences. The authors reviewed 405 hosts’, guests’ and neighbours’ stories and coded the identified forms of misbehaviours into categories. The typology thus developed was validated in the context of the Uber Rides service.

Findings
User misbehaviours in the SE context can be distinguished based on the domain in which the user role is violated and the nature of violated norms. These two conceptual distinctions delineate a four-fold typology of user misbehaviours: illegal, unprofessional, unbefitting and uncivil behaviours.

Research limitations/implications
The trustworthiness of the stories could not be assessed.

Practical implications
The presented typology can be used as a mapping tool that facilitates detection of the full scope of misbehaviours and as a managerial tool that provides ideas for effective management of misbehaviours that correspond to each category.

Originality/value
The paper presents the first empirically derived comprehensive typology of user misbehaviours in SE settings. This typology enables classification of a broad set of misbehaviours, including previously overlooked unprofessional behaviours carried out by peer-service providers. The study also puts forward a revised definition of consumer misbehaviours that encompasses the impact of misbehaviours on parties not directly involved in the SE-mediated exchange.
Young peoples’ environmentally sustainable tourism attitude and responsible behavioral intention
Authors: Schönherr Sarah , Pikkemaat Birgit 
Published: July 2023

Purpose
Environmental tourism impacts are considered to be unbalanced. The implementation of environmental sustainability focuses on restoring a balance through environmentally responsible behavior. As Generation Z was found in recent studies to exhibit divergent levels of environmentally responsible behavior compared to other generations, but also to have intra-generational differences, this study aims to explore the underlying reasons and explanations behind their attitudes and behavioral attempts.

Design/methodology/approach
Four focus group discussions with young people belonging to Generation Z allow to probe deep into their environmental sustainability orientation.

Findings
The results of this study demonstrate that Generation Z’s environmental attitude is triggered by social pressure, social media, as well as by the COVID-19 pandemic. For their touristic behavioral intention, they concentrate predominantly on climate-friendly travel and on-site mobility, waste avoidance as well as emphasizing economic and social sustainability. By furthermore highlighting Generation Z’s responsibility ascription to the supply and the demand side, in addition to illustrating the demand for framework conditions to be created from governments and destinations, the knowledge scope on environmental sustainability is expanded.

Originality/value
In particular, this study enriches environmentally responsible behavior research by incorporating the perspective of Generation Z, while it deepens Generation Z’s behavioral understanding. Furthermore, the results of this study allow to derive implications for tourism policymakers.
Unlocking the restraint—Development of a behaviour change intervention to increase the provision of modified constraint‐induced movement therapy in stroke rehabilitation
Authors: Weerakkody Ashan , Emmanuel Robyn , White Jocelyn , Godecke Erin , Singer Barby 
Published: July 2023

AbstractBackgroundStrong evidence supports the provision of modified constraint‐induced movement therapy (mCIMT) to improve upper limb function after stroke. A service audit identified that very few patients received mCIMT in a large subacute, early‐supported discharge rehabilitation service. A behaviour change intervention was developed to increase the provision of mCIMT following an unsuccessful ‘education only’ attempt. This paper aims to systematically document the steps undertaken and to provide practical guidance to clinicians and rehabilitation services to implement this complex, yet effective, rehabilitation intervention.MethodsThis clinician behaviour change intervention was developed over five stages and led by a working group of neurological experts (n = 3). Data collection methods included informal discussions with clinicians and an online survey (n = 35). The staged process included reflection on why the first attempt did not improve the provision of mCIMT (stage 1), mapping barriers and enablers to the Theoretical Domains Framework (TDF) and behaviour change wheel (BCW) to guide the behaviour change techniques (stages 2 and 3), developing a suitable mCIMT protocol (stage 4), and delivering the behaviour change intervention (stage 5).ResultsReflection among the working group identified the need for upskilling in mCIMT delivery and the use of a behaviour change framework to guide the implementation program. Key determinants of behaviour change operated within the TDF domains of knowledge, skills, environmental context and resources, social role and identity, and social influences. Following the development of a context‐specific mCIMT protocol, the BCW guided the behaviour change intervention, which included education, training, persuasion, environmental restructuring, and modelling.ConclusionThis paper provides an example of using the TDF and BCW to support the implementation of mCIMT in a large early‐supported discharge service. It outlines the suite of behaviour change techniques used to influence clinician behaviour. The success of this behaviour change intervention will be explored in future research.
Forensic uncertainty, fragile remains, and DNA as a panacea: an ethnographic observation of the challenges in twenty‐first‐century Disaster Victim Identification
Authors: Easthope Lucy 
Published: July 2023

AbstractThis is an account of ethnographic research examining the specialist scientific processes known as ‘Disaster Victim Identification’ (DVI) in three settings: Québec, the United States, and the United Kingdom. In cases of multiple deaths, a series of actions accompanied by a plethora of tools are often invoked, housed at a disaster scene, forensic laboratories, a family assistance centre, and a mortuary. In this article, I examine a process dedicated to connecting the biological remains of the deceased with a confirmed validation of personhood. I describe a situation where responders/scientists will attempt multiple testing and re‐testing of human remains, often pushing boundaries of available science. I argue that the search for certainty in identification lies at the heart of the activation of DVI processes, particularly when it is connected to DNA testing. Observing intimate forensic settings and the bricolage of the forensic anthropologist's labour has allowed me to track the production of the science of identity. I then reflect on the wider implications of these observations for affected communities and the responding scientists. Finally, I argue that there is complexity and ambivalence surrounding the increased use of technologies when applied to identification of victims.
A case of anti‐neutrophil cytoplasmic antibody‐associated vasculitis masquerading as Sjögren syndrome
Authors: Pillay S. Sujitha , Nune Arvind , Manzo Ciro , Barman Bhupen , Raza Hasnain 
Published: July 2023

AbstractAnti‐neutrophil cytoplasmic antibody (ANCA) ‐positive vasculitis is a small‐vessel vasculitis that affects multiple body systems. Salivary gland involvement in ANCA‐associated vasculitis is rare. When present, it mimics infection or malignancy, which might lead to misdiagnosis. In this report, we describe a 72‐year‐old man who presented with parotid and submandibular gland pain and swelling in addition to dry mouth and eyes. He had bilateral non‐tender parotid gland lumps and no lymphadenopathies. Laboratory tests were positive for ANCA, hematuria, and proteinuria but negative for Anti‐Ro and ‐La. He was treated with corticosteroids and cyclophosphamide for acute kidney injury. Unfortunately, the patient died a few months later. This case report sheds light on a rare manifestation of salivary gland involvement in ANCA‐associated vasculitis that mimics Sjögren syndrome and the challenges associated with its diagnosis and treatment.
Designing a New Civic Economy? On the Emergence and Contradictions of Participatory Experimental Urbanism
Authors: Thompson Matthew , Lorne Colin 
Published: July 2023

AbstractCan we remake local economies from scratch – not through political struggle but by design – to solve wicked problems and transform urban governance? Such questions are raised by an emergent trend within urban experimentation that emphasises participation and commoning in designing peer‐to‐peer provisioning systems through a platform logic. This article deconstructs the discourses animating what we term “participatory experimental urbanism” and reflects on what this might mean for local state restructuring in times of neoliberal austerity. By following its policies and prototypes as they move and mutate across the London Boroughs of Lambeth and Barking & Dagenham, we examine two exemplary initiatives, Open Works and Participatory City, tracing their beginnings in Lambeth's “cooperative council” model and their ongoing assembling into novel public‐common‐philanthropic partnerships. Foregrounding the contradictions within this latest turn towards urban governance‐beyond‐the‐state, we draw out the implications for the future of social innovation, design‐thinking, and the experimental city.
Anthelmintic nitazoxanide protects against experimental pulmonary fibrosis
Authors: Chen Xu‐Yang , Dong Yan‐Chao , Yu Yuan‐Yuan , Jiang Man , Bu Wen‐Jie , Li Ping , Sun Zhi‐Jie , Dong De‐Li 
Published: July 2023

AbstractBackground and PurposeNitazoxanide is a therapeutic anthelmintic drug. Our previous studies found that nitazoxanide and its metabolite tizoxanide activated adenosine 5′‐monophosphate‐activated protein kinase (AMPK) and inhibited signal transducer and activator of transcription 3 (STAT3) signals. As AMPK activation and/or STAT3 inhibition are targets for treating pulmonary fibrosis, we hypothesized that nitazoxanide would be effective in experimental pulmonary fibrosis.Experimental ApproachThe mitochondrial oxygen consumption rate of cells was measured by using the high‐resolution respirometry system Oxygraph‐2K. The mitochondrial membrane potential of cells was evaluated by tetramethyl rhodamine methyl ester (TMRM) staining. The target protein levels were measured by using western blotting. The mice pulmonary fibrosis model was established through intratracheal instillation of bleomycin. The examination of the lung tissues changes were carried out using haematoxylin and eosin (H&E), and Masson staining.Key ResultsNitazoxanide and tizoxanide activated AMPK and inhibited STAT3 signalling in human lung fibroblast cells (MRC‐5 cells). Nitazoxanide and tizoxanide inhibited transforming growth factor‐β1 (TGF‐β1)‐induced proliferation and migration of MRC‐5 cells, collagen‐I and α‐smooth muscle cell actin (α‐SMA) expression, and collagen‐I secretion from MRC‐5 cells. Nitazoxanide and tizoxanide inhibited epithelial–mesenchymal transition (EMT) and inhibited TGF‐β1‐induced Smad2/3 activation in mouse lung epithelial cells (MLE‐12 cells). Oral administration of nitazoxanide reduced the bleomycin‐induced mice pulmonary fibrosis and, in the established bleomycin‐induced mice, pulmonary fibrosis. Delayed nitazoxanide treatment attenuated the fibrosis progression.Conclusions and ImplicationsNitazoxanide improves the bleomycin‐induced pulmonary fibrosis in mice, suggesting a potential application of nitazoxanide for pulmonary fibrosis treatment in the clinic.
Sampling local ant diversities and the importance of trait analyses
Authors: Probst Rodolfo S. , Silva Rogério R. , Brandão Carlos R. F. 
Published: July 2023

AbstractAnts are an incredibly diverse and ubiquitous group of invertebrates in most terrestrial ecosystems. Although extensively sampled, the majority of ant inventories do not evaluate the effect of different sampling techniques in capturing nontraditional metrics of diversity. We aimed to quantify TD (taxonomic) and FD (functional) diversities for a local ant assemblage by integrating metrics and evaluating complementarity of pitfall traps and Winkler extractors for the leaf litter versus epigeic ant faunas and to determine the effect of sampling techniques on functional composition (community‐weighted means of 11 morphological traits) and functional diversity (multi‐trait morphospace measured with three different metrics). We sampled the local community in an Atlantic Forest fragment using week‐long pitfall traps and 1m2 leaf litter samples submitted to Winkler extractors and quantified the contribution on the technique to uniquely capture the ant morphospace by applying a new index (PWindex). Although ant TD overlapped, FD was significantly affected by the sampling technique. By controlling for TD effects, the community collected by each technique was differentially structured. Higher TD did not translate into wider morphospace for Winklers. Pitfalls recovered more functionally overdispersed assemblages. Pitfalls and Winklers overlapped in the sampling of the overall community, but each sampling method contributed with a unique spectrum to the ant morphospace. Our results suggest the importance of incorporating FD metrics in local ant inventories and the importance of sampling techniques when measuring the magnitude of FD and community structure. Our PWindex further illuminates sampling effects for ant assemblages.Abstract in Portuguese & Spanish is available with online material.
Whole‐brain monosynaptic inputs to lateral periaqueductal gray glutamatergic neurons in mice
Authors: Ma Wei‐Xiang , Li Lei , Kong Ling‐Xi , Zhang Hui , Yuan Ping‐Chuan , Huang Zhi‐Li , Wang Yi‐Qun 
Published: July 2023

AbstractObjectiveThe lateral periaqueductal gray (LPAG), which mainly contains glutamatergic neurons, plays an important role in social responses, pain, and offensive and defensive behaviors. Currently, the whole‐brain monosynaptic inputs to LPAG glutamatergic neurons are unknown. This study aims to explore the structural framework of the underlying neural mechanisms of LPAG glutamatergic neurons.MethodsThis study used retrograde tracing systems based on the rabies virus, Cre‐LoxP technology, and immunofluorescence analysis.ResultsWe found that 59 nuclei projected monosynaptic inputs to the LPAG glutamatergic neurons. In addition, seven hypothalamic nuclei, namely the lateral hypothalamic area (LH), lateral preoptic area (LPO), substantia innominata (SI), medial preoptic area, ventral pallidum, posterior hypothalamic area, and lateral globus pallidus, projected most densely to the LPAG glutamatergic neurons. Notably, we discovered through further immunofluorescence analysis that the inputs to the LPAG glutamatergic neurons were colocalized with several markers related to important neurological functions associated with physiological behaviors.ConclusionThe LPAG glutamatergic neurons received dense projections from the hypothalamus, especially nuclei such as LH, LPO, and SI. The input neurons were colocalized with several markers of physiological behaviors, which show the pivotal role of glutamatergic neurons in the physiological behaviors regulation by LPAG.
The impact of quetiapine monotherapy or in combination with lithium on the thyroid function in patients with bipolar depression: A retrospective study
Authors: Kong Lingzhuo , Shen Yuting , Hu Shaohua , Lai Jianbo 
Published: July 2023

AbstractObjectiveThis study aims to investigate whether quetiapine monotherapy or in combination with lithium significantly disturbs thyroid function in depressed patients with bipolar disorder (BD), and whether difference exists in the post‐treatment thyroid function between the two therapies.MethodsBased on the electric medical records, outpatients and inpatients with a current depressive episode of BD from January 2016 to December 2022 were screened. All patients were treated with quetiapine monotherapy or in combination with lithium. In addition to the demographic data and depression scale, thyroid profiles including total thyroxine (TT4), total triiodothyronine (TT3), free thyroxine (FT4), free triiodothyronine (FT3), thyroid‐stimulating hormone (TSH), thyroid peroxidase antibody (TPOAb), and antithyroglobulin antibody (TGAb) were recorded, analyzed, and compared before and after the treatment.ResultsTotally, 73 eligible patients were enrolled, including 53 in the monotherapy group (MG) and 20 in the combined therapy group (CG). No significant differences in thyroid profiles were detected between the two groups at the baseline (p > 0.05). After one‐month treatment, in the MG, serum levels of TT4, TT3, FT4, and FT3 reduced significantly (p < 0.05), while TSH, TPOAb, and TGAb increased significantly (p < 0.05). In the CG, serum levels of TT4, TT3, and FT4 reduced and TSH increased following one‐month treatment (p < 0.05), with no significant change in FT3, TPOAb, or TGAb (p > 0.05). After one‐month treatment, no difference of TT4, TT3, FT4, FT3, and TSH was found between the two groups (p > 0.05).ConclusionBoth quetiapine monotherapy and a combined therapy with lithium significantly disturbed thyroid function in patients with bipolar depression, while quetiapine monotherapy seems to be associated with immune dysregulation in the thyroid.
Factors explaining individual differences in the oral perception of capsaicin, l‐menthol, and aluminum ammonium sulfate
Authors: Roukka Sulo , Puputti Sari , Aisala Heikki , Hoppu Ulla , Seppä Laila , Sandell Mari 
Published: July 2023

AbstractThis research focused on the oral perception of naturally occurring chemical food compounds that are used in the pharma and food industries due to their pharmacological properties. They stimulate chemically sensitive receptors of the somatosensory system and are also chemesthetic compounds. Capsaicin is a naturally occurring alkaloid activating pungency perception. l‐Menthol is a cyclic monoterpene working also as a medical cooling agent. Aluminum ammonium sulfate is used as a dehydrating agent and additive known to activate astringency in oral cavity. The objective of the study was to identify factors explaining individual differences in the perception of oral chemesthesis measured as sensitivity to chemesthetic compounds and their recognition. The subjects (N = 205) evaluated quality‐specific prototypic compounds at five different concentration levels. Differences between gender were discovered in capsaicin sensitivity with men being less sensitive than women. Age was associated with the perception of capsaicin, l‐menthol, aluminum ammonium sulfate, and the combined oral chemesthetic sensitivity. Quality‐specific recognition ratings were also contributing to the sensitivity to chemesthetic compounds. A combined oral chemesthetic recognition score was created based on quality‐specific recognition ratings. Increasing age generally indicated weaker recognition skills. Better recognizers had a higher combined oral chemesthetic sensitivity score than poorer recognizers. These results provide new information about chemesthesis. The results suggest that age and gender are important factors in explaining individual differences in sensitivity to capsaicin, l‐menthol, and aluminum ammonium sulfate. In addition, recognition skills are associated with the sensitivity based on the quality‐specific recognition scores.