bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety of del Nido cardioplegia during frozen elephant trunk procedure: A case report of first experience in complex aortic surgery
Authors: Comentale Giuseppe , Pilato Emanuele 
Published: February 2023

We report the case of a 74 years-old male who underwent frozen elephant trunk due to a huge aortic arch aneurysm related to a type 1A endoleak after thoracic endovascular aortic repair where cardioplegic arrest was achieved using the Del Nido cardioplegia.
Can radiological findings detect neuropathic pain in patients with osteoarthritis of the knee undergoing surgery?
Authors: Ogawa Hiroyasu , Nakamura Yutaka , Sengoku Masaya , Shimokawa Tetsuya , Sohmiya Kazuki , Ohnishi Kazuichiro , Matsumoto Kazu , Akiyama Haruhiko 
Published: February 2023

Objectives The mechanism underlying neuropathic pain (NP) in osteoarthritis (OA) of the knee is not completely understood. This study aimed to investigate whether possible NP in patients with knee OA undergoing knee surgery is associated with specific radiological findings. Methods This study included 197 patients who underwent knee surgery for symptomatic knee OA. Clinical evaluation was performed using the Central Sensitization Inventory (CSI), Western Ontario and McMaster Universities Arthritis Index (WOMAC), and PainDETECT questionnaire. Radiological evaluation was performed using the hip-knee-ankle (HKA) angle, posterior tibial slope (PTS), varus and valgus laxities, and magnetic resonance imaging OA Knee Score (MOAKS). Radiological findings were compared between patients with possible and unlikely NP. Logistic regression analysis was performed to identify the predictive factors for NP. Results There were 163 and 34 patients with unlikely NP and with possible NP, respectively. The percentage of patients with CSI score ≥ 40 was significantly higher in the possible NP group than in the unlikely NP group (17.6% vs. 6.1%). Patients with possible NP had worse WOMAC scores than patients with unlikely NP. There were no significant positive associations between the possible NP and radiological findings in knee OA. Regression analysis showed no predictive factors for possible NP. Conclusions Possible NP is not associated with specific radiological findings in knee OA. Patients with possible NP may mediate CS and experience more severe symptoms, including decreased knee function and lower quality of life, than patients with unlikely NP.
Does preappointment gabapentin affect neurological examination findings? A prospective, randomized and blinded study in healthy cats
Authors: de Azevedo André F , Veronezi Tayná M , Zardo Izadora L , Ferronatto João VB , Franck Kirian R , Spiering Adriana G , Nunes Luciana N , da Costa Fernanda VA 
Published: February 2023

Objectives The aim of this study was to evaluate the influence of a preappointment oral dose of gabapentin on the neurological examination of cats. Methods A prospective, randomized and blinded clinical trial was conducted in 35 client-owned healthy cats. Cats were scheduled for two appointments and randomly assigned to receive either a placebo or a 100 mg gabapentin capsule prior to the second veterinary visit. A neurological examination was performed during each visit, and the results were compared between groups. Normal/abnormal response rates for each test were based on the number of cats that allowed the test to be performed. Results Gabapentin was administered to 17 cats. Gait and postural reactions were significantly affected in the gabapentin group. Comparing the gabapentin with the placebo groups, proprioceptive ataxia was identified in 4/17 (23.5%) vs 0/18 cats ( P = 0.0288); paw placement deficits were seen in 10/11 (90.9%) vs 1/4 (25%) cats; table tactile placement deficits were identified in 13/17 (76.5%) vs 0/18 cats ( P <0.0001); hopping deficits were seen in 5/17 (29.4%) vs 0/16 cats ( P = 0.0185); and abnormalities on wheelbarrowing and extensor postural thrust were reported in 5/17 (29.4%) vs 0/18 cats ( P = 0.0129). These results had no correlation with age or dose/kg received. No significant difference was noted in the assessment of level and content of consciousness, posture, cranial nerves and spinal nerves. No significant differences were noted in test compliance or examination duration. Conclusions and relevance Gabapentin significantly altered gait analyses and postural reactions in this group of healthy cats. The administration of gabapentin could lead to false–positive results and, possibly, an incorrect identification of neurological lesions. In contrast, gabapentin did not impair the assessment of cranial nerves and spinal reflexes, which can be assessed in patients receiving the drug.
Flexural behavior of different types of steel-concrete composite decks with perfobond rib or head stud shear connectors
Authors: Shan Yulin , Chen Yuli , Zong Zhouhong , Liu Lu , Li Jiaqi , Li Zhuo 
Published: February 2023

With the advent of long-span bridges, steel-concrete composite decks are increasingly concerning structures to address the fatigue problem, wear of the surfacing, and corrosion of the steel decks. The different types of ribs result in the variety of the cross-sections of the composite decks. Thus, the flexural behavior of different types of steel-concrete composite decks with perfobond rib (PBL) or head stud shear connectors was investigated in this study. The different types of composite decks included steel-concrete composite decks (SCCDs), open-rib stiffened steel-concrete composite decks (OSCCDs), and U-rib stiffened steel-concrete composite decks (USCCDs). For this purpose, the static flexural tests of two USCCDs with PBL or head stud shear connectors were conducted. Parametric analyses were also carried out via finite element models to investigate the effects of the number of ribs, the thickness of the ribs, the thickness of the steel plate, the thickness of the concrete slab, and the spacing of the shear connectors on the flexural behavior of the SCCDs, OSCCDs, and USCCDs with PBL or head stud shear connectors. The results indicated that the types of ribs showed significant influence on the flexural behavior of the composite decks. For the same type of shear connector, the flexural behavior of the USCCDs was significantly higher than that of the SCCDs and OSCCDs. Compared with the SCCDs with PBL or head stud shear connectors, the initial stiffness and ultimate load of the USCCDs with PBL or head stud shear connectors were increased by 314.2% and 354.5%, 62.2%, and 89.3%, respectively. The flexural behavior of the composite decks with PBL connections was larger than that of the composite decks with head stud shear connectors for one type of composite deck. The thickness of the concrete slab showed the highest impact on the flexural behavior of the composite decks, followed by the thickness of the rib, the number of ribs, and the spacing of the shear connector. The thickness of the steel plate showed a slight influence on the flexural behavior of the composite decks. The ultimate load of three types of composite decks with PBL or head stud shear connectors was increased by more than 46.5% when the thickness of the concrete slab increased from 80 mm to 120 mm. The reduction stiffness method considering the slip effect was proposed to accurately predict the elastic deflections of three types of composite decks. This study can provide some reference for the design and engineering application of the different types of composite decks.
Intraoperative Acoustic Evaluation of Living Human Knee Cartilage-Comparison with Respect to Cartilage Degeneration and Aging
Authors: Nakagawa Yasuaki , Mori Koji , Mukai Shogo , Shinya Yuki , Nakamura Ryota , Takahashi Motoi 
Published: February 2023

Objective The objective of the study was to evaluate the mechanical properties of living human knee cartilage using our ultrasonic device, and to compare the measurements with respect to cartilage degeneration and aging. Design A total of 95 knees which had undergone arthroscopic knee surgery, from 88 patients, were included in the study, with informed consent. All procedures were reviewed and approved by the ethical committee of our hospital. In the study group, there were 41 men, 47 women, 39 right knees, and 56 left knees. The conditions primarily included knee osteoarthritis and anterior cruciate ligament rupture. The mean operative age was 44.1 years old (range = 10-83). We compared mechanical properties of the knee cartilage with respect to aging and gender, in comparison with normal cartilage. A P value of <0.05 represented statistical significance. Results In the context of the International Cartilage Repair Society (ICRS) classification of cartilage degeneration (grade 0-3), the signal intensity in grade 0 was significantly larger than that in grade 1, 2, or 3. The thickness in grade 0 was significantly higher than that in grade 1, 2, or 3. Normal cartilage in older women had the lowest signal intensity and the least cartilage thickness among all the groups. Conclusion The ultrasonic system we developed was able to detect early degenerative changes in living cartilage in knees. The lowest signal intensity and least cartilage thickness in normal cartilage among older women were correlated to a large prevalence of knee osteoarthritis in women. Level of Evidence: Level IV, case series
Prevalence and factors associated with anxiety and depression among community-dwelling older adults in Hunan, China: a cross-sectional study
Authors: Lu Lulu , Shen Hongxian , Tan Liwen , Huang Qiuping , Chen Qiongni , Liang Mining , He Li , Zhou Yang 
Published: February 2023

Abstract
Background
Older adults’ psychological health is a public health issue that cannot be ignored, especially when these psychological health problems and related factors change across different social backgrounds because of rapid changes in traditions and family structures and the epidemic responses after the outbreak of COVID-19 in China. The aim of our study is to determine the prevalence of anxiety and depression and their associated factors among community-dwelling older adults in China.

Methods
A cross-sectional study was conducted from March to May 2021 with 1173 participants aged 65 years or above from three communities in Hunan Province, China who were selected using convenience sampling. A structured questionnaire including sociodemographic characteristics, clinical characteristics, the Social Support Rating Scale (SSRS), the 7-Item Generalized Anxiety Disorder scale (GAD-7), and the Patient Health Questionnaire-9 Item (PHQ-9) was used to collect relevant demographic and clinical data and to measure social support status, anxiety symptoms, and depressive symptoms, respectively. Bivariate analyses were conducted to explore the difference in anxiety and depression based on samples’ different characteristics. The multivariable logistic regression analysis was performed to test for significant predictors of anxiety and depression.

Results
The prevalence of anxiety and depression were 32.74% and 37.34%, respectively. Multivariable logistic regression analysis revealed that being female, being unemployed before retirement age, lacking physical activity, having physical pain, and having three or more comorbidities were significant predictors for anxiety. Subjective social support and support utilization were significant protective factors. Regarding depression, religion, lacking physical activity, having physical pain, having three or more comorbidities were found to be significant predictors. Support utilization was a significant protective factor.

Conclusions
The study group showed a high prevalence of anxiety and depression. Gender, employment status, physical activity, physical pain, comorbidities, and social support were associated with psychological health problems of older adults. These findings suggest that governments should focus on the psychological health problems of older adults by raising community awareness of issues related to older adults’ psychological health. They should also screen for anxiety and depression among high-risk groups and encourage individuals to seek supportive counseling.
Effects of a family-based lifestyle intervention on co-physical activity and other health-related outcomes of fathers and their children: the ‘Run Daddy Run’ intervention
Authors: Latomme Julie , Morgan Philip J. , Chastin Sebastien , Brondeel Ruben , Cardon Greet 
Published: February 2023

AbstractBackground:Fathers are important in establishing healthy behaviors in their children, but are rarely engaged in lifestyle programs. Focusing on physical activity (PA) of both fathers and their children by engaging them together in PA (i.e. “co-PA”) is therefore a promising novel strategy for interventions. The study aim was to investigate the effect of the ‘Run Daddy Run’ on co-PA and PA of fathers and their children, and secondary outcomes such as weight status and sedentary behaviour (SB).Methods:This study is a non-randomized controlled trial (nRCT), including 98 fathers and one of their 6 to 8 years old children (intervention = 35, control = 63). The intervention was implemented over a 14-week period, and consisted of six (inter)active father-child sessions and an online component. Due to COVID-19, only 2/6 sessions could be implemented as planned, the remaining sessions were delivered online. In November 2019-January 2020 pre-test measurements took place, and post-test measurements in June 2020. Additional follow-up test was conducted in November 2020. PA (i.e. LPA, MPA, VPA and volume) of fathers and children were objectively measured using accelerometry, co-PA and the secondary outcomes were questioned using an online questionnaire.Results:Significant intervention effects were found for co-PA (+ 24 min./day in the intervention compared to the control group, p = 0.002), and MPA of the father (+ 17 min./day, p = 0.035). For children, a significant increase in LPA (+ 35 min./day, p < 0.001) was found. However, an inverse intervention effect was found for their MPA and VPA (-15 min./day, p = 0.005 and − 4 min./day, p = 0.002, respectively). Also decreases in fathers’ and children’s SB were found (-39 min./day, p = 0.022 and − 40 min./day, p = 0.003, respectively), but no changes in weight status, the father-child relationship, and the PA-family health climate (all p > 0.05).Conclusion:The Run Daddy Run intervention was able to improve co-PA, MPA of fathers and LPA of children, and decreasing their SB. Inverse intervention effects were however found for MPA and VPA of children. These results are unique given their magnitude and clinical relevance. Targeting fathers together with their children might be a novel and potential intervention strategy to improve overall physical activity levels, however, further efforts should however be made to target children’s MPA and VPA. Last, replicating these findings in a randomized controlled trial (RCT) is recommended for future research.Trial registration number:This study is registered as a clinical trial (clinicaltrials.gov, ID number: NCT04590755, date: 19/10/2020).
Towards a clinically-based common coordinate framework for the human gut cell atlas: the gut models
Authors: Burger Albert , Baldock Richard A. , Adams David J. , Din Shahida , Papatheodorou Irene , Glinka Michael , Hill Bill , Houghton Derek , Sharghi Mehran , Wicks Michael , Arends Mark J. 
Published: February 2023

Abstract
Background
The Human Cell Atlas resource will deliver single cell transcriptome data spatially organised in terms of gross anatomy, tissue location and with images of cellular histology. This will enable the application of bioinformatics analysis, machine learning and data mining revealing an atlas of cell types, sub-types, varying states and ultimately cellular changes related to disease conditions. To further develop the understanding of specific pathological and histopathological phenotypes with their spatial relationships and dependencies, a more sophisticated spatial descriptive framework is required to enable integration and analysis in spatial terms.

Methods
We describe a conceptual coordinate model for the Gut Cell Atlas (small and large intestines). Here, we focus on a Gut Linear Model (1-dimensional representation based on the centreline of the gut) that represents the location semantics as typically used by clinicians and pathologists when describing location in the gut. This knowledge representation is based on a set of standardised gut anatomy ontology terms describing regions in situ, such as ileum or transverse colon, and landmarks, such as ileo-caecal valve or hepatic flexure, together with relative or absolute distance measures. We show how locations in the 1D model can be mapped to and from points and regions in both a 2D model and 3D models, such as a patient's CT scan where the gut has been segmented.

Results
The outputs of this work include 1D, 2D and 3D models of the human gut, delivered through publicly accessible Json and image files. We also illustrate the mappings between models using a demonstrator tool that allows the user to explore the anatomical space of the gut. All data and software is fully open-source and available online.

Conclusions
Small and large intestines have a natural “gut coordinate” system best represented as a 1D centreline through the gut tube, reflecting functional differences. Such a 1D centreline model with landmarks, visualised using viewer software allows interoperable translation to both a 2D anatomogram model and multiple 3D models of the intestines. This permits users to accurately locate samples for data comparison.
Correlation of psoas major muscle morphology with function and clinical symptoms in patients with symptomatic multilevel lumbar spinal stenosis
Authors: Hou Xiaofei , Hu Hailiang , Kong Chao , Li Yongjin , Zhang Sitao , Wang Wei , Lu Shibao 
Published: February 2023

Abstract
Objective
This study was performed to quantify the morphological characteristics of the psoas major muscle in patients with symptomatic multilevel degenerative lumbar spinal stenosis (SMLSS) and assess the correlations of these morphological characteristics with function and clinical symptoms.

Methods
One hundred fourteen patients diagnosed with SMLSS (≥ 3 segments) were included. The patients’ presenting symptoms were assessed with the Oswestry Disability Index (ODI), and visual analogue scale (VAS) scores were recorded. The morphology of the psoas major was evaluated at the L3/4 intervertebral disc level in three ways: by measuring (i) the psoas muscle mass index (PMI); (ii) the mean muscle attenuation (Hounsfield units, HU); and (iii) the morphologic change of the psoas major (mean ratios of the short axis to the long axis of the bilateral psoas major).

Results
Men had a higher PMI than women (p = 0.001). Patients with severe disability had a significantly lower PMI (p = 0.002) and muscle attenuation (p = 0.001). The PMI and muscle attenuation were significantly higher in the patients with no or mild back pain (both p < 0.001). In the univariable and multivariable analyses, a greater HU value was associated with a higher functional status as assessed by the ODI (p = 0.002), and a higher PMI was associated with less severe back pain as measured by the VAS score (p < 0.001).

Conclusion
This study showed that muscle attenuation of psoas major positively correlated with the functional status and PMI negatively correlated with low back pain severity in patients diagnosed with SMLSS. Future prospective studies are needed to evaluate whether improvement in such muscle parameters through physiotherapy programs can alleviate the clinical symptoms and improve the functional status of patients with SMLSS.
Co-exposure to Anaplasma spp., Coxiella burnetii and tick-borne encephalitis virus in sheep in southern Germany
Authors: Bauer Benjamin Ulrich , Runge Martin , Schneider Melanie , Könenkamp Laura , Steffen Imke , Rubel Wiebke , Ganter Martin , Schoneberg Clara 
Published: February 2023

AbstractThe intracellular bacteria Anaplasma spp. and Coxiella burnetii and the tick-borne encephalitis virus (TBEV) are tick-transmitted pathogens circulating in the southern German sheep population. Knowledge of interaction among Anaplasma spp., C. burnetii and TBEV in sheep is lacking, but together they might promote and reinforce disease progression. The current study aimed to identify co-exposure of sheep to Anaplasma spp., C. burnetii and TBEV. For this purpose, 1,406 serum samples from 36 sheep flocks located in both southern German federal states, Baden-Wuerttemberg and Bavaria, were analysed by ELISAs to determine the antibody levels of the three pathogens. Inconclusive and positive results from the TBEV ELISA were additionally confirmed by a serum neutralisation assay. The proportion of sheep with antibodies against Anaplasma spp. (47.2%), C. burnetii (3.7%) and TBEV (4.7%) differed significantly. Significantly more flocks with Anaplasma spp. seropositive sheep (91.7%) were detected than flocks with antibodies against TBEV (58.3%) and C. burnetii (41.7%), but there was no significant difference between the number of flocks which contained TBEV and C. burnetii seropositive sheep. Seropositivity against at least two pathogens was detected in 4.7% of sheep from 20 flocks. Most co-exposed sheep had antibodies against Anaplasma spp./TBEV (n = 36), followed by Anaplasma spp./C. burnetii (n = 27) and Anaplasma spp./C. burnetii/TBEV (n = 2). Only one sheep showed an immune response against C. burnetii and TBEV. Flocks with sheep being positive against more than one pathogen were widely distributed throughout southern Germany. The descriptive analysis revealed no association between the antibody response of the three pathogens at animal level. Taking the flocks as a cluster variable into account, the exposure to TBEV reduced the probability of identifying C. burnetii antibodies in sheep significantly (odds ratio 0.46; 95% confidence interval 0.24–0.85), but the reason for this is unknown. The presence of Anaplasma spp. antibodies did not influence the detection of antibodies against C. burnetii and TBEV. Studies under controlled conditions are necessary to evaluate any possible adverse impact of co-exposure to tick-borne pathogens on sheep health. This can help to clarify rare disease patterns. Research in this field may also support the One Health approach due to the zoonotic potential of Anaplasma spp., C. burnetii and TBEV.
Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility
Authors: Nash Tessa J. , Morris Katrina M. , Mabbott Neil A. , Vervelde Lonneke 
Published: February 2023

AbstractEnteroids are miniature self-organising three-dimensional (3D) tissue cultures which replicate much of the complexity of the intestinal epithelium. We recently developed an apical-out leukocyte-containing chicken enteroid model providing a novel physiologically relevant in vitro tool to explore host–pathogen interactions in the avian gut. However, the replicate consistency and culture stability have not yet been fully explored at the transcript level. In addition, causes for the inability to passage apical-out enteroids were not determined. Here we report the transcriptional profiling of chicken embryonic intestinal villi and chicken enteroid cultures using bulk RNA-seq. Comparison of the transcriptomes of biological and technical replicate enteroid cultures confirmed their high level of reproducibility. Detailed analysis of cell subpopulation and function markers revealed that the mature enteroids differentiate from late embryonic intestinal villi to recapitulate many digestive, immune and gut-barrier functions present in the avian intestine. These transcriptomic results demonstrate that the chicken enteroid cultures are highly reproducible, and within the first week of culture they morphologically mature to appear similar to the in vivo intestine, therefore representing a physiologically-relevant in vitro model of the chicken intestine.