bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of intravenous tranexamic acid administration in medial opening-wedge distal tibial tuberosity osteotomy (MOWDTO) for varus knee osteoarthritis: a randomized control trial
Authors: Iseki Takuya , Iseki Tomoya , Kanto Ryo , Onishi Shintaro , Yoshiya Shinichi , Tachibana Toshiya , Nakayama Hiroshi 
Published: March 2023

Abstract
Background
This randomized controlled study was undertaken to investigate the efficacy of intravenous tranexamic acid (TXA) administration in reducing perioperative blood loss in patients undergoing medial opening-wedge distal tibial tuberosity osteotomy (MOWDTO). It was hypothesized that TXA would reduce perioperative blood loss in MOWDTO.

Methods
A total of 61 knees in 59 patients who underwent MOWDTO during the study period were randomly assigned to either of the groups with intravenous TXA administration (TXA group) or without TXA administration (control group). In the TXA group, patients received 1000 mg of TXA intravenously before skin incision and 6 h after the first dose. The primary outcomes was the volume of perioperative total blood loss which calculated using the blood volume and hemoglobin (Hb) drop. The Hb drop was calculated as the difference between preoperative Hb and postoperative Hb at days 1, 3, and 7.

Results
The perioperative total blood loss was significantly lower in the TXA group (543 ± 219 ml vs. 880 ± 268 ml, P < 0.001). The Hb drop was significantly lower at postoperative days 1, 3 and 7 in the TXA group than in the control group (day 1: 1.28 ± 0.68 g/dl vs. 1.91 ± 0.69 g/dl, P = 0.001; day 3: 1.54 ± 0.66 g/dl vs. 2.69 ± 1.00 g/dl, P < 0.001; day 7: 1.74 ± 0.66 g/dl vs. 2.83 ± 0.91 g/dl, P < 0.001).

Conclusion
Intravenous TXA administration in MOWDTO could reduce the perioperative blood loss.
Trial registration The study was approved by the institutional review board. (Registered on 26/02/2019 Registration Number 3136).
Level of Evidence Level I, randomized controlled trial.
HPV genotypes in high-grade cervical lesions and invasive cervical carcinoma detected in Gabonese women
Authors: Moussavou-Boundzanga Pamela , Koumakpayi Ismaël Hervé , Engohan Aloghe Corinne , Chansi Junie K. , Revignet Rose , Leroy Eric M. , Berthet Nicolas 
Published: March 2023

Abstract
Background
Cervical cancer is the third most common cancer among women worldwide, but particularly affects women living in sub-Saharan Africa. Screening and vaccination programs are two prevention approaches that can reduce cervical cancer incidence. However, effective vaccination campaigns require better knowledge of the prevalence of the main human papillomavirus (HPV) genotypes reported in high-grade neoplastic lesions and invasive carcinomas in women.

Methods
All samples collected in this study were processed using standard histopathological methods with haematoxylin and eosin staining of the sections. Areas with abnormal cells were then identified. The HPV genotype was determined on the DNA extracted from the same sections using nested PCR followed by amplicon sequencing and real-time PCR specific to five different HPV genotypes (16, 18, 33, 45 and 58).

Results
A total of 132 Gabonese patients with high-grade neoplastic lesions were included in this study; 81% were squamous cell carcinomas (SCC). At least one HPV was detected in 92.4% patients; HPV16 (75.4%) was the most frequent genotype, followed by HPV18, 58, 45, 33 and 35. Moreover, histological analysis showed that SCC samples had 50% and 58.2% stage III and IV tumor cells, respectively, according to the FIGO classification. Finally, 36.9% of these stage III and IV patients were less than 50 years old.

Conclusions
Our results confirm the high prevalence of HPV16 and 18 genotypes among high-grade lesions in Gabonese women. This study confirms the need for a national strategy for early screening of precancerous lesions associated with a broad national vaccination program among non-sexually active women to significantly reduce the long-term cancer burden.
Functional identification of two novel carbohydrate-binding modules of glucuronoxylanase CrXyl30 and their contribution to the lignocellulose saccharification
Authors: Liu Jiawen , Zhu Jingrong , Xu Qian , Shi Rui , Liu Cong , Sun Di , Liu Weijie 
Published: March 2023

Abstract
Background
Glycoside hydrolase (GH) family 30 xylanases are a distinct group of xylanases, most of which have a highly specific catalytic activity for glucuronoxylan. Since GH30 xylanases do not normally carry carbohydrate-binding modules (CBMs), our knowledge of the function of their CBMs is lacking.

Results
In this work, the CBM functions of CrXyl30 were investigated. CrXyl30 was a GH30 glucuronoxylanase containing tandem CBM13 (CrCBM13) and CBM2 (CrCBM2) at its C terminus, which was identified in a lignocellulolytic bacterial consortium previously. Both CBMs could bind insoluble and soluble xylan, with CrCBM13 having binding specificity for the xylan with l-arabinosyl substitutions, whereas CrCBM2 targeted l-arabinosyl side chains themselves. Such binding abilities of these two CBMs were completely different from other CBMs in their respective families. Phylogenetic analysis also suggested that both CrCBM13 and CrCBM2 belong to novel branches. Inspection of the simulated structure of CrCBM13 identified a pocket that just accommodates the side chain of 3(2)-alpha-l-arabinofuranosyl-xylotriose, which forms hydrogen bonds with three of the five amino acid residues involved in ligand interaction. The truncation of either CrCBM13 or CrCBM2 did not alter the substrate specificity and optimal reaction conditions of CrXyl30, whereas truncation of CrCBM2 decreased the kcat/Km value by 83% (± 0%). Moreover, the absence of CrCBM2 and CrCBM13 resulted in a 5% (± 1%) and a 7% (± 0%) decrease, respectively, in the amount of reducing sugar released by the synergistic hydrolysis of delignified corncob whose hemicellulose is arabinoglucuronoxylan, respectively. In addition, fusion of CrCBM2 with a GH10 xylanase enhanced its catalytic activity against the branched xylan and improved the synergistic hydrolysis efficiency by more than fivefold when delignified corncob was used as substrate. Such a strong stimulation of hydrolysis resulted from the enhancement of hemicellulose hydrolysis on the one hand, and the cellulose hydrolysis is also improved according to the lignocellulose conversion rate measured by HPLC.

Conclusions
This study identifies the functions of two novel CBMs in CrXyl30 and shows the good potential of such CBMs specific for branched ligands in the development of efficient enzyme preparations.
Cross-domain coordination of resource allocation and route planning for the edge computing-enabled multi-connected vehicles
Authors: Xue Duan , Guo Yan , Li Ning , Song Xiaoxiang , Zhang Lixiong 
Published: March 2023

AbstractMulti-access edge computing (MEC) has unique interests in processing intensive and delay-critical computation tasks for vehicular application through computation offloading. However, due to the spatial inhomogeneity and dynamic mobility of connected vehicles (CVs), the edge servers (ESs) must dynamically adjust their resource allocation schemes to effectively provide computation offloading services for CVs. In this case, we propose a MEC framework supporting the collaboration of CVs, and incorporate digital twins (DTs) into wireless network to mitigate the unreliable long-distance communication between CVs and ESs. To solve the contradiction between the task change requirements of CVs and ES resources, we proactively balance the computation resources load of ESs by appropriately cooperative route planning of CVs, and achieve cross-domain load balancing between traffic flow and edge cloud resources domains. Furthermore, we jointly formulate route planning and resource allocation to balance the travel and service time delay by considering the mobility of CVs, distributed resources of ESs and the deadline sensitive vehicular tasks comprehensively. Besides, considering the coupled relationship between route planning and resource allocation, an alternating optimization algorithm is proposed to solve the formulated problem. we decompose it into two sub-problems. Firstly, a reinforcement learning method is used to optimize the route planning of CVs with fixed resource allocation. Then, an online learning and iterative algorithm is used to optimize the resource allocation strategy of edge cloud with fixed route selection. In order to demonstrate that our suggested scheme is more effective than other comparison schemes, a comprehensive series of experiments are conducted.
Genomic analysis of firework fear and noise reactivity in standard poodles
Authors: Handegård Karin Westereng , Storengen Linn Mari , Joergensen Dina , Lingaas Frode 
Published: March 2023

Abstract
Background
Fear of firework noises and other loud, sudden noises (noise reactivity) is a significant problem for many dogs and may have a negative effect on both welfare and, in severe cases, the life expectancy of dogs. A wide range of behavior traits, including fear-related behaviors, have high heritability estimates in dogs. The aim of this study was to estimate genomic heritability for fear of fireworks and loud noises in dogs.

Results
A genomic heritability estimate was performed based on genome-wide SNPs from standard poodles with records of fear of fireworks and noise reactivity. The study was based on questionnaires answered by owners, who also volunteered to return a cheek swab from their dog for DNA analyses. SNP-based heritability was estimated to be 0.28 for firework fear and 0.16 for noise reactivity. We also identified an interesting region on chromosome 17 that was weakly associated with both traits.

Conclusions
We have estimated low to medium genomic heritabilities for fear of fireworks and noise reactivity in standard poodles. We have also identified an interesting region on chromosome 17, which harbors genes that have been shown to be involved in different psychiatric traits with anxiety components in humans. The region was associated with both traits; however, the association was weak and need further verification from other studies.
DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation
Authors: Gómez Rodolfo , Barter Matt J. , Alonso-Pérez Ana , Skelton Andrew J. , Proctor Carole , Herrero-Beaumont Gabriel , Young David A. 
Published: March 2023

AbstractBackgroundKnowledge about regulating transcription factors (TFs) for osteoblastogenesis from mesenchymal stem cells (MSCs) is limited. Therefore, we investigated the relationship between genomic regions subject to DNA-methylation changes during osteoblastogenesis and the TFs known to directly interact with these regulatory regions.ResultsThe genome-wide DNA-methylation signature of MSCs differentiated to osteoblasts and adipocytes was determined using the Illumina HumanMethylation450 BeadChip array. During adipogenesis no CpGs passed our test for significant methylation changes. Oppositely, during osteoblastogenesis we identified 2462 differently significantly methylated CpGs (adj. p < 0.05). These resided outside of CpGs islands and were significantly enriched in enhancer regions. We confirmed the correlation between DNA-methylation and gene expression. Accordingly, we developed a bioinformatic tool to analyse differentially methylated regions and the TFs interacting with them. By overlaying our osteoblastogenesis differentially methylated regions with ENCODE TF ChIP-seq data we obtained a set of candidate TFs associated to DNA-methylation changes. Among them, ZEB1 TF was highly related with DNA-methylation. Using RNA interference, we confirmed that ZEB1, and ZEB2, played a key role in adipogenesis and osteoblastogenesis processes. For clinical relevance, ZEB1 mRNA expression in human bone samples was evaluated. This expression positively correlated with weight, body mass index, and PPARγ expression.ConclusionsIn this work we describe an osteoblastogenesis-associated DNA-methylation profile and, using these data, validate a novel computational tool to identify key TFs associated to age-related disease processes. By means of this tool we identified and confirmed ZEB TFs as mediators involved in the MSCs differentiation to osteoblasts and adipocytes, and obesity-related bone adiposity.
Possible mechanisms of action of Bacillus wiedmannii AzBw1, a biocontrol agent of the root-knot nematode, Meloidogyne arenaria
Authors: Fallahzadeh-Mamaghani Vahid , Shahbazi-Ezmareh Roya , Shirzad Akbar , Moslehi Shalaleh 
Published: March 2023

Abstract
Background
With increased environmental concerns and restrictions of chemical control, the importance of other eco-friendly strategies for management of the nematodes is being substantially grown nowadays. One of the most well-known strategies that have attracted the attentions is biological control of these deleterious agents. In our previous study (Moslehi et al. in Egypt J Biol Pest Control 31:1–11, 2021), Bacillus wiedmannii AzBw1 was introduced as a robust antagonistic agent against root-knot nematode Meloidogyne arenaria. Present study addressed the possible mechanisms of action of this strain.

Results
Based on quantitative bioassays it was shown that the strain AzBw1 is able to produce considerable amount of siderophore, protease, and chitinase. In an in vitro assay conducted by bi-plate Petri dishes, it was shown that hatching of the nematode eggs, subjected to bacterial volatile compounds (BVCs) was 34% lower than those of mock-treated control eggs. On the other hand, mortality of BVC-treated juveniles was 33.5% higher than those of mock-treated control juveniles. The secretory proteins from the medium culture of strain AzBw1 were precipitated and fractionated by anion exchange chromatography (AEC). Fractions from AEC were checked for hydrolytic activity and nematicidal effect. It was found that the fractions with the highest protease activity have a strong nematicidal effect. In contrast, significant nematicidal effect in the fraction with Chitinase activity was detected.

Conclusion
The results suggested that protease activity played a key role in strain AzBw1 antagonism against root-knot nematode, M. arenaria. Finally, nonvolatile organic compounds were also extracted from the medium culture after removing secretory proteins and enzymes. Obtained results showed that these metabolites had also a strong anti-nematode effect.
Induction of liver transplant immune tolerance in an outbred rat strain model using tacrolimus
Authors: Park Min-Jung , Na Hyun Sik , Joo Young-Shin , Cho Keun-Hyung , Kim Se-Young , Choi Jeong Won , Baek Jin-Ah , Choi Jong Young , You Young Kyoung , Cho Mi-La 
Published: March 2023

Abstract
Background
Orthotopic liver transplantation is the only option for patients with end-stage liver disease and hepatocellular carcinoma. Post-transplant immunosuppressive therapy is important to prevent graft failure. We investigated the effectiveness of tacrolimus (FK506) and their mechanisms for liver transplant immune tolerance in an outbred rat LT model.

Results
To investigate the therapeutic effect of the FK506 on outbred rat LT model, FK506 and postoperative therapy were administered subcutaneously once or twice daily to transplanted rats. Histopathological and immunohistochemical analyses were conducted for all groups. The regulation of inflammatory cytokine signaling in the spleen was analyzed by flow cytometry. FK506 attenuated allograft rejection and increased survival in rat orthotopic liver transplantation models. The FK506-treated group had reduced serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. Furthermore, FK506 decreased the expression of inflammatory cytokines and the activation of pathogenic Th1 and Th17 cells in the liver.

Conclusions
Taken together, we revealed that FK506 ameliorated strong allograft rejection in outbred liver transplantation model by anti-inflammatory effect and inhibitory peroperty of pathogenic T cells.
The Severity of Congenital Hypothyroidism With Gland-In-Situ Predicts Molecular Yield by Targeted Next-Generation Sequencing
Authors: Levaillant Lucie , Bouhours-Nouet Natacha , Illouz Frédéric , Amsellem Jager Jessica , Bachelot Anne , Barat Pascal , Baron Sabine , Bensignor Candace , Brac De La Perriere Aude , Braik Djellas Yasmine , Caillot Morgane , Caldagues Emmanuelle , Campas Marie-Neige , Caquard Marylène , Cartault Audrey , Cheignon Julie , Decrequy Anne , Delemer Brigitte , Dieckmann Katherine , Donzeau Aurélie , Doye Emilie , Fradin Mélanie , Gaudillière Mélanie , Gatelais Frédérique , Gorce Magali , Hazart Isabelle , Houcinat Nada , Houdon Laure , Ister-Salome Marielle , Jozwiak Lucie , Jeannoel Patrick , Labarthe Francois , Lacombe Didier , Lambert Anne-Sophie , Lefevre Christine , Leheup Bruno , Leroy Clara , Maisonneuve Benedicte , Marchand Isis , Marquant Emeline , Muszlak Matthias , Pantalone Letitia , Pochelu Sandra , Quelin Chloé , Radet Catherine , Renoult-Pierre Peggy , Reynaud Rachel , Rouleau Stéphanie , Teinturier Cécile , Thevenon Julien , Turlotte Caroline , Valle Aline , Vierge Melody , Villanueva Carine , Ziegler Alban , Dieu Xavier , Bouzamondo Nathalie , Rodien Patrice , Prunier-Mirebeau Delphine , Coutant Régis 
Published: March 2023

Abstract

Introduction
Congenital hypothyroidism with gland-in-situ (CH-GIS) is usually attributed to mutations in the genes involved in thyroid hormone production. The diagnostic yield of targeted next-generation sequencing (NGS) varied widely between studies. We hypothesized that the molecular yield of targeted NGS would depend on the severity of CH.

Methods
Targeted NGS was performed in 103 CH-GIS patients from the French national screening program referred to the Reference Center for Rare Thyroid Diseases of Angers University Hospital. The custom targeted NGS panel contained 48 genes. Cases were classified as solved or probably solved depending on the known inheritance of the gene, the classification of the variants according to the American College of Medical Genetics and Genomics, the familial segregation, and published functional studies. Thyroid-stimulating hormone at CH screening and at diagnosis (TSHsc and TSHdg) and free T4 at diagnosis (FT4dg) were recorded.

Results
NGS identified 95 variants in 10 genes in 73 of the 103 patients, resulting in 25 solved cases and 18 probably solved cases. They were mainly due to mutations in the TG (n = 20) and TPO (n = 15) genes. The molecular yield was, respectively, 73% and 25% if TSHsc was ≥ and &lt; 80 mUI/L, 60% and 30% if TSHdg was ≥ and &lt; 100 mUI/L, and 69% and 29% if FT4dg was ≤ and &gt; 5 pmol/L.

Conclusion
NGS in patients with CH-GIS in France found a molecular explanation in 42% of the cases, increasing to 70% when TSHsc was ≥ 80 mUI/L or FT4dg was ≤ 5 pmol/L.
The Role of Religious Thought in Interdisciplinary “Law and Literature” Studies
Authors: Schramm Jan-Melissa 
Published: March 2023

AbstractThis essay traces the intense interrelatedness of the three discourses that we now consider distinct but that ask similar questions about the existential value of life and the relationship between spiritual and temporal matters. Law, literature, and theology address crucial and complementary issues of epistemology—that is, questions of evidence-based knowledge and how we ground our ethical deliberations on these evidential foundations.
Fathers, Mothers, Saints, Martyrs: Religion as a Lineage of Belief
Authors: Coleman Dawn 
Published: March 2023

AbstractCritiquing the literary-critical habit of approaching religion primarily in terms of individual belief, this essay proposes that the sociologist Danièle Hervieu-Léger’s definition of religion as a “lineage of belief” can reorient literary scholars to religion’s investment in its own survival and reproduction. Hervieu-Léger’s model emphasizes that religious institutions ensure their continuity by negotiating intracommunity conflict and intergenerational transformations. Building on this model, the essay argues that literary texts participate in religion’s collective memory and self-definition, then illustrates this point by demonstrating how Harriet Beecher Stowe’s Oldtown Folks (1869) creates a bildungsroman-like narrative to shape the story of Protestantism’s Anglican-Puritan branch from the seventeenth to nineteenth centuries. Although this representation is political in that it reinforces Protestantism as integral to American identity, Oldtown Folks prioritizes the vibrancy and longevity of Anglo-American Puritanism and Episcopalianism as relatively autonomous, family- and community-based institutions that maintain complex relationships to state violence and imperialism. For instance, while Oldtown Folks endorses Protestantism’s collaboration with North American settler colonialism, it also challenges the efficacy and desirability of the Congregational Church’s South Seas missionary work.
The Role of Prolonged Bed Rest in Postoperative Cerebrospinal Fluid Leakage After Surgery of Intradural Pathology—A Retrospective Cohort Study
Authors: Krahwinkel Sophia , Schipmann Stephanie , Spille Dorothee , Maragno Emanuele , Al Barim Bilal , Warneke Nils , Stummer Walter , Gallus Marco , Schwake Michael 
Published: March 2023

BACKGROUND:
Postoperative cerebrospinal fluid leakage (CSFL) is a feared complication after surgery on intradural pathologies and may cause postoperative complications and subsequently higher treatment costs.

OBJECTIVE:
To assess whether prolonged bed rest may lower the risk of CSFL.

METHODS:
We performed a retrospective cohort study including patients with intradural pathologies who underwent surgery at our department between 2013 and 2021. Cohorts included patients who completed 3 days of postoperative bed rest and patients who were mobilized earlier. The primary end point was the occurrence of clinically proven CSFL.

RESULTS:
Four hundred and thirty-three patients were included (female [51.7%], male [48.3%]) with a mean age of 48 years (SD ±20). Bed rest was ordered in 315 cases (72.7%). In 7 cases (N = 7/433, 1.6%), we identified a postoperative CSFL. Four of them (N = 4/118) did not preserve bed rest, showing no significant difference to the bed rest cohort (N = 3/315; P = .091). In univariate analysis, laminectomy (N = 4/61; odds ratio [OR] 8.632, 95% CI 1.883-39.573), expansion duraplasty (N = 6/70; OR 33.938, 95% CI 4.019-286.615), and recurrent surgery (N = 5/66; OR 14.959, 95% CI 2.838-78.838) were significant risk factors for developing CSFL. In multivariate analysis, expansion duraplasty was confirmed as independent risk factor (OR 33.937, 95% CI 4.018-286.615, P = .001). In addition, patients with CSFL had significant higher risk for meningitis (N = 3/7; 42.8%, P = .001).

CONCLUSION:
Prolonged bed rest did not protect patients from developing CSFL after surgery on intradural pathologies. Avoiding laminectomy, large voids, and minimal invasive approaches may play a role in preventing CSFL. Furthermore, special caution is indicated if expansion duraplasty was done.