bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determining the earliest growth stage to detect the presence of endophytes in tall fescue and perennial ryegrass seedlings using molecular markers
Authors: Lee Kendall , Hill Nicholas , Dela Cerna Chloe , Missaoui Ali 
Published: June 2023

AbstractBackgroundTall fescue (Festuca arundinacea [Schreb.], Lolium arundinaceum [Schreb.] Darbysh) and perennial ryegrass (Lolium perenne) are important cool‐season forage and amenity grasses that have a mutualistic association with an endophytic fungus. Endophytes confer insect and drought resistance to plants but can produce mammalian toxins. Novel endophytes that do not produce mammalian toxins have been introduced to elite cultivars for commercial production. Seed companies need to maintain adequate levels of novel endophytes within the elite forage cultivars. Endophyte detection is performed using immunochemical and molecular techniques because of their speed and reliability. Early detection in seedlings is essential to evaluate the viability of the endophyte within seed lots.MethodsThis research aimed to identify the earliest growth stage in which immunochemical and molecular methods can detect viable endophyte in seedlings of tall fescue cultivars BarOptima (e34), Texoma MaxQII (584), and Jesup MaxQ (542), as well as the perennial ryegrass cultivar Remington (NEA2).ResultsImmunochemical testing detected endophytes in seedlings 14 days after germination (DAG), but the detection rate increased until 42 DAG in some cultivars tested. The molecular marker Tef1exon detected endophytes at a lower rate than the immunochemical method at 28–42 DAG. However, there was insufficient DNA to detect endophytes in 14 DAG seedlings using markers.ConclusionsWe conclude that the most accurate detection of viable endophytes in seedlings was 42 DAG, at which sufficient and consistent endophyte colonization occurred.
College credit plus modality and future matriculation of high school students in Ohio
Authors: Tipton Kristy L. 
Published: June 2023

AbstractCollege Credit Plus (CCP) is a dual enrollment program allowing high school students to obtain both high school credit and college credit by participating in college courses at a local higher education institution. CCP courses can be taken at a college or university, at the student's high school, or online. This research seeks to find if CCP course modality affects future matriculation to the same university following high school. The population of CCP student data was a historical sample collected from the spring semester of 2018 to avoid the influence of COVID‐19 on attendance. Data on the location of CCP courses that the student was enrolled in and whether the student chose to attend the university beyond high school were collected. Chi‐square and the post hoc test, Cramer's V, were used to analyze the data, resulting in a significant association between CCP course modality and future university matriculation. CCP student proximity and familiarity with the university campus may influence the choice to remain at the same institution after high school graduation. These findings can be used to direct further CCP research with a wider scope.Practical Takeaways
Students who complete College Credit Plus courses on the postsecondary campus, rather than at their high school or online, are more likely to matriculate to that same institution after high school graduation.
This may be a result of success on the university campus, interactions and relationships with faculty and staff, and peer relationships.
More research is needed on other factors influencing CCP student matriculation after high school, such as proximity to campus, GPA, student activities and involvement, number of credits completed, and successful courses.
Assessment of thyroid cartilage ossification pattern in cancer patients: A suggestion of active ossification by tumor progression
Authors: Sung You‐Na , Kim Yeseul , Cho Kyung‐Ja , Sim Jongmin 
Published: June 2023

AbstractBackgroundOssified cartilage is much more susceptible to cancer infiltration, but the reason remains unknown, and the relationship between the ossification pattern and cancer infiltration has not been studied.MethodsThe presence of thyroid cartilage ossification, cancer infiltration, ossification pattern (usual: direction from inferior to superior; unusual: other than the usual pattern), and distance between cancer and ossified cartilage were evaluated in laryngectomy specimens.ResultsThere were 28 and 27 cases of usual and unusual patterns, respectively. There was no association between ossification pattern and cancer infiltration. However, the distance between the ossified area and cancer cells was greater in the usual pattern than in the unusual pattern (p = 0.006). And the usual pattern was more frequently observed in cases with a distance >1 mm than in cases with cancer infiltration or a distance ≤1 mm (p = 0.004).ConclusionThese results suggest the possibility of an active ossification due to tumor progression.
Adherence to infection prevention and control measures and risk of exposure among health‐care workers: A cross‐sectional study from the early period of COVID‐19 pandemic in Addis Ababa, Ethiopia
Authors: Weldetinsae Abel , Alemu Zinabu A. , Tefaye Kirubel , Gizaw Melaku , Alemahyehu Ermias , Tayachew Adamu , Derso Sisay , Abate Moa , Getachew Mesaye , Abera Daniel , Mebrhatu Arone , Kefale Higu , Habebe Shambel , Assefa Tsigereda , Mekonnen Aderajew , Tollera Getachew , Tessema Masresha 
Published: June 2023

AbstractBackground and AimHealthcare workers (HCWs) are considered a high‐risk group for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) exposure, ascribed to the amount of time they spend in health‐care facilities (HCFs). This study aimed to assess HCWs' compliance with Infection Prevention and Control (IPC) procedures and the risk of exposure during the early period of the pandemic in Addis Ababa, Ethiopia.MethodsA descriptive cross‐sectional survey was conducted from June to September 2020. With a response rate of 79.2%, a standardized questionnaire was administered among 247 HCWs, working in eight HCFs. Descriptive and multivariate regression analysis was carried out in STATA version 16.ResultsAbout 22.5% (55) of the HCWs had proper adherence to IPC procedures. Of the total participants, 28.2% (69) had proper use of Personal Protective Equipment (PPE), 40% (98) had proper hand hygiene practices, and 33.1% (81) had frequently cleaned their working environment. HCWs who received training on IPC protocols were four times more likely to follow IPC standards than those with no training (adjusted odds ratio [AOR] = 3.93; 95% confidence interval [CI]: 1.46, 10.58). Besides, HCWs working in treatment centers were four times more likely to follow IPC standards than those working in conventional hospitals (AOR = 3.61; 95% CI:1.63, 8.02). Nurses were four times more likely to have adherence to IPC measures than cleaners and runners (AOR = 4.37; 95% CI: 1.38–13.88).ConclusionThe nature and magnitude of the pandemic did not introduce the required degree of adherence to IPC procedures, per se does not match the level of diligence needed to halt SARS‐CoV‐2 transmission. Our finding suggested that providing periodic training of HCWs with particular emphasis on nonclinical staff is commendable. Furthermore, it is necessary to maintain resilent IPC in HCF through continous follow up and safety drills, to assess the readiness of HFCs' adherance to IPC measures under normal circumstances, which could improve prepardeness for an effective response during epidemics.
Assessing the effect of energy consumption and food production from agriculture on environmental degradation in Pakistan: Does institutional quality matter?
Authors: Ashraf Junaid , Javed Aiman , Bin Jawaid Zaid , Shovkat Shamuratov 
Published: June 2023

Abstract
The nexus between food production (FP), energy consumption (EC), institutional quality (IQ), and ecological degradation (ED) has important implications for environmental sustainability. Nevertheless, environmental degradation caused by FP is widely ignored, especially in Pakistan. To fill this void, we investigated the impact of energy use, FP, and IQ on Pakistan's environmental degradation between 1990 and 2021. We also explore the moderating influence of IQ on ED. To evaluate this influence, we use the autoregressive distributed lag (ARDL) method for cointegration and the fully modified ordinary least-squares method for robustness estimation. Our empirical results demonstrate that, whereas FP and EC contribute to pollution, IQ reduces the pace of environmental degradation. Furthermore, the presence of a strong IQ mitigates the harmful ecological effects of FP and EC. Moreover, the results revealed that unbiased domestic IQ is crucial to boosting energy and agricultural efficiency while reducing CO2 emissions. Our findings might be considered while public policies to reduce carbon emissions and increase FP in a healthy environment are being developed. Integr Environ Assess Manag 2024;20:518–532. © 2023 (SETAC)
Assessment of the clinical outcomes and complications of hysteroscopic and laparoscopic approaches in the treatment of symptomatic isthmocele: An observational study
Authors: Fatehnejad Mina , Hadizadeh Alireza , Tayebi Amirhossein , Ayati Aryan , Marjani Narjes , Gheshlaghi Parand , Asgari Zahra , Hosseini Reihaneh 
Published: June 2023

AbstractObjectivesTo investigate the outcomes of patients undergoing laparoscopic or hysteroscopic approaches for isthmoplasty.MethodsA total of 99 isthmocele patients with an average age of 38.45 ± 4.72 years were included in the 2 years of this retrospective cohort study. Forty‐five underwent laparoscopic and 54 underwent hysteroscopic isthmocele excision and myometrial repair.ResultsPain scores were significantly higher in the hysteroscopy group before the procedure, but there were no significant pain score differences after the surgery. In 1 year of follow up, dysmenorrhea and dyspareunia were higher among hysteroscopy patients. Furthermore, hysteroscopy significantly improved postmenstrual spotting after surgery better than laparoscopy, but in the follow up, there was no significant difference between the two groups in this regard (mean rank for hysteroscopy vs. laparoscopy: 32.30 vs. 37.48, U = 418, P = 0.29).ConclusionIn patients with a history of infertility, ectopic pregnancy, lower gravidity, lower parity, and a lower number of cesarean sections, laparoscopic isthmoplasty is preferred over the hysteroscopic approach. Both methods have similar effects on midcycle vaginal bleeding, duration of postmenstrual spotting, and pain. However, a higher rate of dyspareunia and dysmenorrhea could be associated with hysteroscopy.
Development of robust perovskite single crystal radiation detectors with high spectral resolution through synergetic trap deactivation and self‐healing
Authors: Wang Lixiang , Song Yilong , Li Liqi , Tao Liting , Yan Minxing , Bi Weihui , Yang Xueying , Sun Yuan , Dong Qingfeng , Yang Deren , Fang Yanjun 
Published: June 2023

AbstractOrganic–inorganic halide perovskite single crystals (SCs) are promising materials for detecting ionizing radiation owing to their outstanding photoelectric conversion capability and inexpensive solution processability. However, the accuracy and stability of the detectors have been limited due to the charge traps and defects in SCs, especially when operated under high‐precision photon‐counting mode for energy spectrum acquisition. Here, we proposed a trap freezing deactivation route, which obviously suppressed dark current and noise by up to 97% and 92%, respectively. Furthermore, the bulk ion migration effect was essential for the ability to instantly self‐heal defects induced by radiation damage at temperatures down to −30°C. Consequently, the detector exhibits a record high energy resolution of 7.5% at 59.5 keV for 241Am γ‐ray source, which is the best solution‐processed semiconductor radiation detectors at the same energy range. In addition, the detector maintains over 90% of its initial performance after 9 months of storage when tested in the air. Our results will represent a revision of the paradigm that high‐spectral‐resolution and robust radiation detectors can only be realized with high temperature grown inorganic semiconductor single crystals.image
Injectable and self‐healing dual crosslinked gelatin/kappa‐carrageenan methacryloyl hybrid hydrogels via host‐guest supramolecular interaction for wound healing
Authors: Yilmaz‐Aykut Dilara , Torkay Gulsah , Kasgoz Alper , Shin Su Ryon , Bal‐Ozturk Ayca , Deligoz Huseyin 
Published: June 2023

AbstractInjectable hydrogels based on natural polymers have shown great potential for various tissue engineering applications, such as wound healing. However, poor mechanical properties and weak self‐healing ability are still major challenges. In this work, we introduce a host‐guest (HG) supramolecular interaction between acrylate‐β‐cyclodextrin (Ac‐β‐CD) conjugated on methacrylated kappa‐carrageenan (MA‐κ‐CA) and aromatic residues on gelatin to provide self‐healing characteristics. We synthesize an MA‐κ‐CA to conjugate Ac‐β‐CD and fabricate dual crosslinked hybrid hydrogels with gelatin to mimic the native extracellular matrix (ECM). The dual crosslinking occurs on the MA‐κ‐CA backbone through the addition of KCl and photocrosslinking process, which enhances mechanical strength and stability. The hybrid hydrogels exhibit shear‐thinning, self‐healing, and injectable behavior, which apply easily under a minimally invasive manner and contribute to shear stress during the injection. In‐vitro studies indicate enhanced cell viability. Furthermore, scratch assays are performed to examine cell migration and cell–cell interaction. It is envisioned that the combination of self‐healing and injectable dual crosslinked hybrid hydrogels with HG interactions display a promising and functional biomaterial platform for wound healing applications.
Interactions Between HR‐pQCT Bone Density and D3Cr Muscle Mass (or HR‐pQCT Bone Structure and HR‐pQCT Muscle Density) in Predicting Fractures: The Osteoporotic Fractures in Men Study
Authors: Kirk Ben , Harrison Stephanie L , Zanker Jesse , Burghardt Andrew J , Orwoll Eric , Duque Gustavo , Cawthon Peggy M 
Published: June 2023

ABSTRACTWe examined if an interaction exists between bone and muscle in predicting fractures in older men. Prospective data from the Osteoporotic Fractures in Men study was used to build Cox proportional hazards models. Predictors included HR‐pQCT total volumetric BMD (Tt.BMD), trabecular BMD (Tb.BMD), cortical BMD (Ct.BMD) and cortical area (Ct.Ar) at distal radius/tibia, HR‐pQCT muscle volume and density (diaphyseal tibia), D3‐creatine dilution (D3Cr) muscle mass, and grip strength and leg force, analyzed as continuous variables and as quartiles. Incident fractures were self‐reported every 4 months via questionnaires and centrally adjudicated by physician review of radiology reports. Potential confounders (demographics, comorbidities, lifestyle factors, etc.) were considered. A total of 1353 men (mean age 84.2 ± 4.0 years, 92.7% white) were followed for 6.03 ± 2.11 years. In the unadjusted (continuous) model, there were no interactions (p > 0.05) between any muscle variable (D3Cr muscle mass, muscle volume, muscle density, grip strength or leg force) and Tt.BMD at distal radius/tibia for fractures (all: n = 182–302; nonvertebral: n = 149–254; vertebral: n = 27–45). No consistent interactions were observed when interchanging Tt.BMD for Tb.BMD/Ct.BMD or for Ct.Ar (bone structure) at the distal radius/tibia in the unadjusted (continuous) models. Compared with men in quartiles (Q) 2–4 of D3Cr muscle mass and Q2–4 of distal tibia Tt.BMD, men in Q1 of both had increased risk for all fractures (hazard ratio (HR) = 2.00; 95% confidence interval [CI] 1.24–3.23, p = 0.005) and nonvertebral fractures (HR = 2.10; 95% CI 1.25–3.52, p < 0.001) in the multivariable‐adjusted model. Confidence intervals overlapped (p > 0.05) when visually inspecting other quartile groups in the multivariable‐adjusted model. In this prospective cohort study of older men, there was no consistent interactions between bone and muscle variables on fracture risk. Larger sample sizes and longer follow‐up may be needed to clarify if there is an interaction between bone and muscle on fracture risk in men. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Ferulic acid exerts a protective effect against cyclosporine‐induced liver injury in rats via activation of the Nrf2/HO‐1 signaling, suppression of oxidative stress, inflammatory response, and halting the apoptotic cell death
Authors: Nouri Ali , Ghatreh‐Samani Keihan , Amini‐Khoei Hossein , Najafi Mohammad , Heidarian Esfandiar 
Published: June 2023

AbstractDrug‐induced liver injury is one of the main challenges that leads to the withdrawal of several drugs in the clinical setting. Cyclosporine is one of the drugs that its long‐term administration exerts devastating effects on the hepatocytes. In the present study, we aimed to evaluate the effect of ferulic acid, a natural compound found in plants, on cyclosporine‐mediated hepatotoxicity. Forty‐eight male Wistar rats were treated with cyclosporine and/or ferulic acid to evaluate the function as well as the morphology of liver cells. We found that ferulic acid dose‐dependently recovered the functional as well as histopathological parameters of liver cells, as revealed by the improvement of hepatocellular vacuolation, portal fibroplasia, and necrosis. Moreover, this phenolic compound was able to restore the balance of the redox system in cyclosporine‐treated rats by activating the nuclear factor (NF) erythroid 2‐related factor 2 (Nrf2)/hemeoxygenase‐1 (HO‐1) signaling axis. Of note, the protective effects of ferulic acid against cyclosporine‐mediated liver toxicity were not restricted only to induction of the potential antioxidant property, as in the presence of this agent, the expression of pro‐inflammatory cytokines such as NF‐κB, tumor necrosis factor (TNF)‐α, and interleukin‐1β was also diminished. Ferulic acid also shifted the equilibrium between the expression levels of proapoptotic to antiapoptotic proteins and thereby prevented the development of cyclosporine‐induced liver injury. Overall, these findings highlighted that ferulic acid can reduce cyclosporine‐induced liver injury due to its antioxidant properties.
Generation of Vgll4‐DreER transgenic mouse for visualizing and manipulating VGLL4‐expressing cells in vivo
Authors: Zhong Yazhu , Tan Xixi , Wang Xiaodong , Jiang Jun , Song Kai , Chen Haiyuan , Zhang Hao , Wang Zuoyun , Zhang Lei , Guo Chunming , Liang Hongfeng , Yu Wei 
Published: June 2023

AbstractVestigial like family member 4 (VGLL4), a member of the Hippo pathway, is a transcriptional cofactor involved in many biological processes, such as tumor progression, postnatal heart growth, and muscle regeneration. However, the VGLL4 expression pattern in vivo remains unclear. To detect and trace Vgll4‐expressing cells and their progeny, we generated and characterized a new tamoxifen‐inducible Dre knock‐in mouse line, Vgll4‐DreER. This mouse line expressed DreER (Dre recombinase fused to the estrogen receptor) under the control of the endogenous Vgll4 promoter. After crossing the Vgll4‐DreER mouse line with the Dre‐responsive reporter H11‐rRFP, Dre‐mediated recombination in the tissue was monitored on the basis of red fluorescent protein (RFP) signals, which indicated the distribution of VGLL4‐positive cells in vivo. Our data revealed that VGLL4 is widely expressed in various cell types at embryonic and neonatal stages. After comparison with our previously reported Vgll4‐GFP mouse, we found that the RFP signal profile was wider than the green fluorescent protein (GFP) pattern, indicating that Vgll4‐DreER is more sensitive for labeling VGLL4‐expressing cells. We next used a dual‐recombination system to simultaneously label VGLL4‐ and keratin 5 (KRT5)‐positive cell populations, and no crosstalk was observed in the Krt5‐CreER;Vgll4‐DreER;R26‐rGlR mice. Taken together, the Vgll4‐DreER mouse line is a valuable new tool for examining the precise VGLL4 expression profile and conditional manipulating of VGLL4‐expressing cells and their progeny.
Consumers prefer natural medicines more when treating psychological than physical conditions
Authors: Li Tianyi , Gal David 
Published: June 2023

AbstractConsumers generally prefer natural to synthetic drugs, a phenomenon known as the “natural preference.” Through six experiments and one archival study, the current research shows that while consumers have a general preference for natural drugs over synthetic drugs, this preference is stronger when the goal is to treat psychological rather than physical conditions. Process evidence indicates an important mechanism that explains the amplified natural preference for treating psychological conditions: Consumers are more concerned about their true selves being altered when treating psychological conditions, and they perceive natural drugs to be less likely than synthetic drugs to affect their true selves. The current research provides novel insights into the natural preference. It also offers policy and managerial implications for marketing natural remedies and pharmacological treatments for mental health conditions.
Experimental analysis of canine behavior and cognition: Introduction to the special issue
Authors: Hall Nathaniel J. , Lazarowski Lucia , Edwards Timothy L. 
Published: June 2023

AbstractOver the last 3 decades, the use and popularity of canid models for basic and applied behavioral research has grown dramatically, and for good reasons. Dogs are uniquely among the human world, living and working in our homes and places of employment while an even greater population lives on the outskirts of human life, scavenging and navigating the city life. This provides a rich continuum of environmental contexts for the canine experience, leading to some unique and even human‐like behaviors in animals. The articles in this special issue provide additional insight into factors that influence canine welfare, methods for evaluating dogs' preferences and the reinforcing effectiveness of stimuli, trick learning and retention, concept learning, and scent detection performance under sparse reinforcement conditions. Here we provide an overview of these articles and their contribution to our understanding of canine behavior.
Evidence of clinical efficacy of a first generation CD19 CAR T cell in B cell malignancies
Authors: Shohdy Kyrillus S. , Pillai Manon , Guest Ryan , Rothwell Dominic , Kirillova Natalia , Chow Shien , Gilham David , Thistlethwaite Fiona , Hawkins Robert 
Published: June 2023

AbstractThe persistence and reactivity of CAR T cells were enhanced by adding co‐stimulatory domains, which is the basis of currently approved CAR‐T cell therapies. However, this comes at the expense of increasing toxicities from the strong cytokine release effect. This is the first report from anti‐CD19 CAR‐T cell therapy with a single activation domain to show a favourable safety profile and clinical efficacy with two patients who achieved durable responses up to 28 months in a cohort with heavily pretreated B cell malignancies.