bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Vitamin D Deficiency on the Expression of Genes and Proteins in Patients With Medium Rotator Cuff Tears
Authors: Kim Hyun Tae , Lee Su Hyun , Lee Jeong Kun , Chung Seok Won 
Published: July 2023

Background: Whether vitamin D deficiency is related to rotator cuff muscle and tendon physiology is controversial. Purpose: To assess the relationship between vitamin D deficiency and various gene expression patterns in patients with rotator cuff tears. Study Design: Controlled laboratory study. Methods: During arthroscopic surgery, samples from the supraspinatus muscle, deltoid muscle, and supraspinatus tendon were acquired from 12 patients with vitamin D deficiency (serum 25-hydroxyvitamin D concentration <20 ng/dL) and 12 patients with sufficient vitamin D levels (control group, serum 25-hydroxyvitamin D concentration ≥30 ng/dL) who were matched for age, sex, and tear size. Alterations in the expression of genes and proteins associated with myogenesis, muscle atrophy, adipogenesis, inflammation, and apoptosis, as well as in vitamin D receptor expression, were assessed using quantitative reverse transcription polymerase chain reaction, Western blotting, and immunohistochemistry and were compared between the 2 groups. Results: Vitamin D receptor gene expression in the deltoid muscle was significantly lower in the vitamin D deficiency group than in the control group ( P = .043). Additionally, in the deltoid muscle, myoDgene expression levels were lower and atrogin levels were higher in the vitamin D deficiency group than in the control group ( P = .034 and P = .011, respectively). However, in the supraspinatus muscle, no differences were observed between groups in the expression of myogenesis- or atrophy-related genes (all P > .05). The expression of inflammation-related genes (interleukin ( IL)-1β and IL-6) was significantly higher in the vitamin D deficiency group, in both the deltoid and supraspinatus muscles (all P < .05). The supraspinatus tendon tissue did not show any significant differences in any gene expression evaluated (all P > .05). A correlation between gene and protein expression was observed for atrogin and IL-1β in the deltoid muscle ( P = .019 and P = .037, respectively) and for IL-6 in the supraspinatus muscle ( P = .044). Conclusion: Vitamin D deficiency was not associated with the expression of myogenesis-related or muscle atrophy–related genes in the supraspinatus muscle of patients with rotator cuff tears, unlike in the deltoid muscle; rather, vitamin D deficiency was associated with increased proinflammatory cytokine expression. Clinical Relevance: In patients with rotator cuff tears, vitamin D deficiency was observed to be associated with increased levels of proinflammatory cytokines in the rotator cuff muscles, without significant changes in gene expression related to myogenesis or muscle atrophy.
Deltoid ligament injury and repair
Authors: Loozen Loek , Veljkovic Andrea , Younger Alastair 
Published: July 2023

The deltoid ligament is the primary stabilizer of the medial side of the ankle joint. It is a complex structure with an origin at the medial malleolus from where it spreads fan shaped distally with an insertion into the medial side of the talus, calcaneus and navicular bone. This chapter gives an overview of the anatomy, function, and pathology of the deltoid ligament. The deltoid ligament can become insufficient as a result of an ankle injury or prolonged strain. In the acute setting, deltoid insufficiency often coincides with multi ligament injury the ankle joint; syndesmosis injury, or ankle fractures. Management in the acute phase remains a subject of debate. Some orthopedic surgeons have a tendency towards repair, whereas most trauma surgeons often treat the deltoid nonoperatively. In the chronic setting the ligament complex is often elongated as a result of prolonged strain. It often coexists with a hindfoot valgus, as is the case in planovalgus feet. In such a case a realignment procedure should be combined with the deltoid repair.
Risk factors for sexually transmitted infections in sexual assault victims attending an Accident and Emergency Department in Hong Kong
Authors: Man Tak Wah , Wong Ying Kit , Lee Iris Yee Chi 
Published: July 2023

Objective: The prevalence of sexually transmitted infections in Hong Kong is not high. The objective of this study was to determine the risk factors that associated with occurrence of sexually transmitted infections in local sexual assault victims, so as to facilitate the counseling and management of victims upon their initial attendance at an Accident and Emergency Department. Methods: This was a retrospective cohort of sexual assault victims presented to Accident and Emergency Department of Kwong Wah Hospital between 1 January 2016 and 31 December 2021. The outcome was the occurrence of sexually transmitted infections. Variables were analyzed using univariate and multivariable analyses. Results: A total of 190 victims were included in the study. Univariate analysis indicated that sexual assault involving multiple perpetrator, oral penetration, and anal penetration were associated with increased occurrence of sexually transmitted infections in the victims (all with p < 0.05). Among these, multiple perpetrator was the only statistically significant risk factor on multivariable analysis ( p < 0.05). Conclusion: Multiple-perpetrator sexual assault was identified as a significant risk factor of sexually transmitted infections. Emergency physicians should consider explaining to victims of multiple-perpetrator sexual assault that they have a higher risk of contracting sexually transmitted infections and emphasizing on the importance of complying with medical treatments, attending follow-up and observing symptoms of sexually transmitted infections. Proper clinical assessment, adequate follow-up, and reassurance for the victims with lower likelihood of contracting sexually transmitted infections are suggested for managing sexual assault victims.
Prediction and influencing factors of photovoltaic system power generation of a single house based on continuously measured data
Authors: Zhang Weirong , Liu Xinyang , Deng Gaofeng , Wang Zhichao 
Published: July 2023

With the development of photovoltaic (PV) power generation systems in single houses, research has recently focused on the prediction of PV power generation to match PV power generation with building energy consumption characteristics. However, prediction models for PV power generation under different weather conditions based on the actual monitoring data of the PV power generation system in a single house are still lacking. The present study is based on the actual monitoring of PV power generation data of a single house with a PV system that was installed in Beijing 5 years ago. We analysed the main weather factors affecting PV power generation and power conversion efficiency (PCE). The results showed a positive correlation between power generation and solar radiation, with the ambient temperature (Ta) and total cloud cover (TCC) being the main weather factors affecting power generation and PCE. Based on the analysis results, a support vector regression (SVR) model was established to predict PV power generation. In addition, the mean absolute error (MAE), mean absolute percentage error (MAPE) and coefficient of variation (R2) of the SVR model were 3.39, 34.7% and 0.86, respectively, which can accurately be used to predict PV power generation under different weather types.
Moisture insensitive analysis of polyester/viscose waste textiles using Near-Infrared spectroscopy and Orthogonalization of external parameters algorithm
Authors: Qiu Xun , Liu Yuanyuan , Zhang Xiaoqiang , Liu Dongzhi , Wang Ran , Wang Chong , Liu Jun , Liu Wei , Gong Yan 
Published: July 2023

Near-Infrared (NIR) spectroscopic analyses can be applied in waste textile recycling as a rapid and non-invasive method to provide both qualitative and quantitative results. However, it has been a challenge to enhance the accuracy rate of NIR-based waste textile sorting due to the major influences from water contexts in the samples. Orthogonalization of External Parameters (EPO) has been introduced to reduce the interference from water absorption in NIR spectral signals for better accuracy and reliability in modeling. Here we explore the feasibility of applying EPO strategy with varieties of algorithms, including partial least squares regression (PLS), artificial neural network (ANN), decision tree (DT), random forest (RF), gradient boosting decision tree (GBDT), extreme random tree (Extra-tree), decision tree model based on AdaBoost algorithm (AdaBoost-tree), support Vector machine (SVM), one-dimensional convolutional neural network (1D-CNN), and one-dimensional convolutional neural network with improved Inception structure (1D-Inception-CNN). 216 waste textiles samples from Xinjiang, China, were studied with different moisture levels. Among them, 80 samples were used to develop the EPO algorithm, 112 were used to establish the prediction models, and 24 were used as test datasets. Then, the samples were scanned using a near-infrared spectrometer at different moisture regain rates. Our results showed that the moisture content of waste textiles had strong absorption peaks near 1150 and 1450 nm, leading to a decrease in the near-infrared reflectance of waste textiles. To verify the effectiveness of the EPO algorithm, the decision coefficients (R2 score) and other indicators of the model without the EPO process and the model with EPO process are systematically compared. Our results show that the EPO algorithm preprocessing improves the accuracy of the NIR model (The average decision coefficient (R2 score) of the models was increased by 0.83), especially when the moisture interference level is significant. Therefore, the EPO integrated modeling method is a reliable approach for better accuracy in NIR-based waste textile sorting.
In Silico Investigation of Aporphine Alkaloids Isolated From Magnolia coriacea Against α-Glucosidase and Tyrosine Phosphatase 1B
Authors: Ninh Pham Thi , Bui Thanh Q. , Dung Nguyen Thi , Sung Tran Van , Phuong Thao Tran Thi , Thu Ha Chu Thi , Thanh Bui Van , Chien Tran Van , Quy Phan Tu , Triet Nguyen Thanh , Thai Nguyen Minh , Nhung Nguyen Thi Ai 
Published: July 2023

Objective Magnolia coriacea is a rare species of Magnoliaceae and categorized as critically endangered, yet known for its valuable pharmaceutical properties. Phytochemical study of the plant was carried out, and the isolated compounds were subjected to different computational platforms to predict their chemical, inhibitory, physicochemical, and pharmacological properties. Methods M coriacea dried twig or leaf powder was partially extracted with n-hexane, ethyl acetate, and 90% methanol to produce the corresponding extracts, which were subjected to column chromatography. The isolated compounds were identified by nuclear magnetic resonance (NMR) spectroscopic and electrospray ionization mass spectrometry (ESI-MS) methods. Results Eight known aporphine alkaloids were isolated, (+)-glaucine N-oxide (1), N-methyl glaucine acetate (2), (+)-( S)-glaucine (3), magnoflorine (4), pontevedrine (5), O-methylatheroline (6), 7-hydroxydehydroglaucine (7), and mangochinine acetate (8). Quantum-based calculations found no abnormal structural constraints and suggested 2, 4, and 8 as the most promising inhibitors of protein structures in general with intensive nucleophilic reactive sites at their nitrogen atoms. Classical-based docking simulation agrees with this with respect to 3W37 (docking score [DS] < −12 kcal·mol−1) and PTP1B (DS ca −13 kcal·mol−1) structures. The biocompatibility and pharmacokinetics of the three candidates given by quantitative structure–activity relationship (QSAR) and absorption, distribution, metabolism, excretion, and toxicity (ADMET) regressions justify their potential for medicinal development. The results encourage further experimental studies of the promising M coriacea-extracted aporphine alkaloids for drug-developing purposes, especially mangochinine acetate (8). Conclusions Isolation and molecular docking simulation of 8 aporphine alkaloids were accomplished. QSAR and ADMET regressions suggested that three compounds (2, 4, and 8) were the most suitable for medicinal applications given both biocompatibility and pharmacokinetics based on specific prediction toward 3W37 (α-glucosidase) and PTP1B (tyrosine phosphatase 1B) structures.
Asymmetric and Lag Effects of Industry Risk Factors on the Malaysian Oil and Gas Stocks
Authors: Hoque Mohammad Enamul , Low Soo-Wah , Zaidi Mohd Azlan Shah , Tee Lain-Tze , Ghazali Noor Azlan 
Published: July 2023

This study examines asymmetric and the lag effects of oil price, gas price, and exchange rate on stock performance of the Malaysian oil and gas sub-industries. Using company-level data and multi factor asset pricing models, this study found that oil price, gas price, exchange rate, and common systematic risk factors such as market, size, and book-to-market have significant effects on the stock performance of Malaysian oil and gas sub-industries, but these exposures remain heterogeneous. Oil price and exchange rate show strong asymmetrical impacts on stock performance when decomposed into oil price increases (decreases) and exchange rate depreciation (appreciation). Increases in oil price and exchange rate depreciation have a greater impact on stock performance of oil and gas industry than decreases in oil price and exchange rate appreciation. Oil price, gas price, and exchange rate exhibit lag effects on the performance of oil and gas stocks. The findings have important asset pricing implications for profitable investment and portfolio strategies based on oil and gas risk factors for oil-exporting country. JEL Classifications: C13; C58; G10; G12; G14; Q4
Dealing with censoring in a network meta-analysis of time-to-event data
Authors: Kalyvas Chrysostomos , Papadimitropoulou Katerina , Malbecq William , Spineli Loukia M. 
Published: July 2023

Background The Health Technology Assessment agencies typically require an economic evaluation considering a lifetime horizon for interventions affecting survival. However, survival data are often censored and are typically analyzed assuming the censoring mechanism independent of the event process. This assumption may lead to biased results when the censoring mechanism is informative. Methods We propose a flexible approach to jointly model the participants experiencing an event and censored participants by incorporating the pattern-mixture (PM) model in the fractional polynomial (FP) model within the network meta-analysis (NMA) framework. We introduce the informative censoring hazard ratio parameter that quantifies the departure from the censored at random assumption. The FP-PM model is exemplified in an NMA of the overall survival from non-small cell lung carcinoma studies using Bayesian methods. Results The results on hazard ratio and survival from the FP-PM model are similar to those from the FP model. However, the posterior standard deviation of the hazard ratio is slightly greater when censored data are modeled because the uncertainty induced by censoring is naturally accounted for in the FP-PM model. The between-study standard deviation is almost identical in both models due to the low censoring rate across the studies. At the end of the corresponding studies, the informative censoring hazard ratio demonstrated a possible departure from the censored at random assumption for gefitinib and best supportive care. Conclusions The proposed method offers a comprehensive sensitivity analysis framework to examine the robustness of the NMA results to clinically plausible censoring scenarios.
A large deletion conferring pale green leaves of maize
Authors: Yao Guoqi , Zhang Hua , Leng Bingying , Cao Bing , Shan Juan , Yan Zhenwei , Guan Haiying , Cheng Wen , Liu Xia , Mu Chunhua 
Published: July 2023

Abstract
Background
The structural basis of chloroplast and the regulation of chloroplast biogenesis remain largely unknown in maize. Gene mutations in these pathways have been linked to the abnormal leaf color phenotype observed in some mutants. Large scale structure variants (SVs) are crucial for genome evolution, but few validated SVs have been reported in maize and little is known about their functions though they are abundant in maize genomes.

Results
In this research, a spontaneous maize mutant, pale green leaf-shandong (pgl-sd), was studied. Genetic analysis showed that the phenotype of pale green leaf was controlled by a recessive Mendel factor mapped to a 156.8-kb interval on the chromosome 1 delineated by molecular markers gy546 and gy548. There were 7 annotated genes in this interval. Reverse transcription quantitative PCR analysis, SV prediction, and de novo assembly of pgl-sd genome revealed that a 137.8-kb deletion, which was verified by Sanger sequencing, might cause the pgl-sd phenotype. This deletion contained 5 annotated genes, three of which, including Zm00001eb031870, Zm00001eb031890 and Zm00001eb031900, were possibly related to the chloroplast development. Zm00001eb031870, encoding a Degradation of Periplasmic Proteins (Deg) homolog, and Zm00001eb031900, putatively encoding a plastid pyruvate dehydrogenase complex E1 component subunit beta (ptPDC-E1-β), might be the major causative genes for the pgl-sd mutant phenotype. Plastid Degs play roles in protecting the vital photosynthetic machinery and ptPDCs provide acetyl-CoA and NADH for fatty acid biosynthesis in plastids, which were different from functions of other isolated maize leaf color associated genes. The other two genes in the deletion were possibly associated with DNA repair and disease resistance, respectively. The pgl-sd mutation decreased contents of chlorophyll a, chlorophyll b, carotenoids by 37.2%, 22.1%, and 59.8%, respectively, and led to abnormal chloroplast. RNA-seq revealed that the transcription of several other genes involved in the structure and function of chloroplast was affected in the mutant.

Conclusions
It was identified that a 137.8-kb deletion causes the pgl-sd phenotype. Three genes in this deletion were possibly related to the chloroplast development, which may play roles different from that of other isolated maize leaf color associated genes.
Plastome evolution and phylogenomic insights into the evolution of Lysimachia (Primulaceae: Myrsinoideae)
Authors: Liu Tong-Jian , Zhang Shu-Yan , Wei Lei , Lin Wei , Yan Hai-Fei , Hao Gang , Ge Xue-Jun 
Published: July 2023

AbstractBackgroundLysimachiaL., the second largest genus within the subfamily Myrsinoideae of Primulaceae, comprises approximately 250 species worldwide. China is the species diversity center ofLysimachia, containing approximately 150 species. Despite advances in the backbone phylogeny ofLysimachia, species-level relationships remain poorly understood due to limited genomic information. This study analyzed 50 complete plastomes for 46Lysimachiaspecies. We aimed to identify the plastome structure features and hypervariable loci ofLysimachia. Additionally, the phylogenetic relationships and phylogenetic conflict signals inLysimachiawere examined.ResultsThese fifty plastomes withinLysimachiahad the typical quadripartite structure, with lengths varying from 152,691 to 155,784 bp. Plastome size was positively correlated with IR and intron length. Thirteen highly variable regions inLysimachiaplastomes were identified. Additionally,ndhB, petBandycf2were found to be under positive selection. Plastid ML trees and species tree strongly supported thatL. maritimaas sister to subg.Palladia + subg.Lysimachia(Christinae clade), while the nrDNA ML tree clearly placedL. maritimaand subg.Palladiaas a sister group.ConclusionsThe structures of these plastomes ofLysimachiawere generally conserved, but potential plastid markers and signatures of positive selection were detected. These genomic data provided new insights into the interspecific relationships ofLysimachia, including the cytonuclear discordance of the position ofL. maritima, which may be the result of ghost introgression in the past. Our findings have established a basis for further exploration of the taxonomy, phylogeny and evolutionary history withinLysimachia.
Two types of GLR channels cooperate differently in light and dark growth of Arabidopsis seedlings
Authors: Krzeszowiec Weronika , Lewandowska Aleksandra , Lyczakowski Jan Jakub , Bebko Kateryna , Scholz Sandra S. , Gabryś Halina 
Published: July 2023

Abstract
Background
GLutamate Receptor-like (GLR) channels are multimeric, ionotropic, ligand-gated plant transmembrane receptors. They are homologous to mammalian glutamate receptors, iGLuRs, which are critical to neuronal function. GLRs have been reported several times to play a role in photomorphogenesis. However, to date, no study has looked at the mechanism of their involvement in this process. Here we focused on examining the impact of GLRs on the regulation of early seedling growth in blue light, red light, and in the dark.

Results
Wild type and six photoreceptor mutant seedlings were grown on media supplemented with known iGLuR/GLR channel antagonists: MK-801, which non-competitively blocks NMDA channels in mammalian cells, and CNQX, known for competitive blocking of AMPA channels in mammalian cells. The lengths of hypocotyls and roots were measured in seedlings of phyA, phyB, phot1, phot2, cry1, and cry2 mutants after 7 days of in vitro culture. Changes in growth parameters, both in light and in darkness upon application of chemical antagonists, show that both types of GLR channels, NMDA-like and AMPA-like, are involved in the regulation of seedling growth irrespective of light conditions. Analysis of seedling growth of photoreceptor mutants indicates that the channels are influenced by signaling from phot1, phot2, and cry1. To extend our analysis, we also evaluated the elicitation of a calcium wave, which is likely to be partially driven by GLRs, in Arabidopsis seedlings. The changes in cellobiose-induced calcium waves observed after applying GLR inhibitors suggest that both types of channels likely cooperate in shaping Arabidopsis seedling growth and development.

Conclusions
Our work provides the first experimental evidence that two types of GLR channels function in plants: NMDA-like and AMPA-like. We also demonstrate that the channels are involved in seedling growth and development, at least partially through modulation of calcium signaling, but they are unlikely to play a major role in photomorphogenesis.