bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does Rising Resources Income, Consumer Prices, Government Outlay, and Globalisation Hinder Africa's Sustainable Development?
Authors: Aladejare Samson Adeniyi , Musa Mohammed Auwal 
Published: May 2023

Abstract
This study examined the long-term effect of contemporary challenges such as growing resource income, rising consumer prices, inefficient public spending, and globalisation on Africa's quest for sustainable development. A robust measure of sustainable development that integrates resource productivity is adopted. The study period spanned from 1991 to 2021, and data sourced from 24 African countries. The empirical output for this study is derived using long-term methodologies, including feasible generalised least squares, Driscoll-Kraay, and panel dynamic ordinary least squares. The estimation of the long-term model was to capture the consequential effects of contemporary issues on sustainable development. Evidence from the model revealed that increases in resources income, consumer prices, public outlay, and globalisation are deterrents to sustainable development. The individual cross-sectional regression outputs also demonstrated similar effects. Given these contemporary challenges, the study proposed relevant policy measures to aid the attainment of sustainable development.
Uncharacterized protein with amino acid deletions from Bombyx mori illustrates divergence from Bombyx mandarina hemocytin and showed modulated gene expression after infection by Nosema bombycis
Authors: Kalyani Dhadesugur , Varghese Alvina , Prabhuling Shambhavi Hungund , Makwana Pooja , Ponnuvel Kangayam M. , Pradeep Appukuttan R 
Published: May 2023

AbstractGenome wide gene expression analysis, transcription analysis and mass spectrometry have revealed several uncharacterized genes and proteins from commercial silkwormBombyx mori. Infection ofB. morilarvae with the microsporidianNosema bombycisinduced appearance of exclusive proteins in hemocytes ofB. morishowing host response. Mass spectrometry of the exclusive proteins revealed abundance of an uncharacterized protein H9JAZ8 that displayed conserved domains of Von Willebrand Factor type D domain (VWF), C8 domain, trypsin inhibitor-like cysteine rich domain and conserved epidermal growth factor like. Protein model of H9JAZ8 confirmed presence of VWF domains, cysteine rich domains and disulphide bonds. Expression ofH9JAZ8gene showed upregulation on day 2 after the infection followed by significant down regulated expression till day 10 exhibiting infection- associated modulation of the gene. BLASTP of H9JAZ8 showed 99% amino acid sequence similarity withB. moriBGIBMGA006693 demonstrating H9JAZ8 as a paralog ofB. morihemocytin. BLASTP of H9JAZ8 revealed 18 most similar hemocytin homologues from different insect species with 99% similarity forB. mandarinahemocytin. A Maximum Parsimony phylogeny tree showed three clusters where H9JAZ8 branched from the hemocytin ofB. mandarinawith 100% bootstrap value. Global BLAST analysis of H9JAZ8 with hemocytin ofB. mandarina(XP_028038271.1) showed deletion of two regions, a 27 amino acid stretch, RRIFFSFILIYCTFNCEAGYGIPASSD at 2–28th position and a 21 amino acid stretch, DVVVISPNHHNNSSYYSYSQG at 317-338th position. Togteher,B. moriH9JAZ8 is diverged fromB. mandarinahemocytin as part of speciation and immune evolution during domestication from the wild silkworm.
Effects of precipitation concentration and human activities on city runoff changes
Authors: Lv Cuimei , Wang Xinrui , ling minhua , Xu Wenjing , Yan Denghua 
Published: May 2023

Abstract

Under the dual influence of precipitation and human activity, urban runoff has changed significantly. To evaluate how these two factors may affect city runoff, we used hydro statistical methods to provide a scientific basis for the evolution of urban flood control and non-point source pollution. This study took Guangzhou city as an example and examined the characteristic changes of runoff from 1970 to 2020. The results showed that the concentration of precipitation in Guangzhou City varied between 0.25 and 0.63 during 1970–2020, with a mean value of 0.47, and abrupt changes in runoff occurred in 2013 and 2016. We also found that the impact of human activities on runoff was greater than that of the concentration of precipitation from to 2014–2016, contributing 82.3% while the contribution of precipitation concentration was 11.7%. The impact of precipitation concentration on runoff was more obvious during 2017–2020, contributing up to 98% of the change in runoff compared to only 2% for human activities, and runoff increased with increasing precipitation concentration. The combined effect of precipitation concentration and human activities has a certain impact on urban runoff, and each has a different impact on urban runoff in different periods. This research provides a scientific basis for alleviating urban flood.
Neurobehavioral deficits, histoarchitectural alterations, parvalbumin neuronal damage and glial activation in the brain of male Wistar rat exposed to Landfill leachate
Authors: Usende Ifukibot Levi , Bintu Mofio M. , Daniella Osinachi Chinonyerem , Oyenike Oyelowo-Abdulraheem Fatima , Agbonu Adikpe Oluwa , Adedamola Azeez Mariam , Ndidi Enefe , Saka Sanni Fatimah , Gela Beselia V. , Mbagwu Smart I. , Edem Edem Ekpenyong , Olukayode Olopade James , James Connor R 
Published: May 2023

Abstract
Concerns about inappropriate disposal of waste into unsanitary municipal solid waste landfills around the world has been on the increase; and this pose a public health challenge due to leachate production. The neurotoxic effect of Gwagwalada landfill leachate (GLL) was investigated in male adult Wistar rats. Rats were exposed to 10% concentration of GLL for 21days. Control group received tap water for same period of experiment. Our results showed that neurobehavior, absolute body and brain weights and brain histomorphology as well as parvalbumin interneurons were severely altered, with consequent astrogliosis and microgliosis after 21 days of administrating GLL. Specifically, there was severe loss and shrinkage of purkinje cells, with their nucleus, and severe diffused vacuolations of the white matter tract of GLL exposed rat brains. There was severe cell loss in the granular layer of the cerebellum resulting to reduced thickness of the layer. Also. there was severe loss of dendritic arborization of the purkinje cells in GLL exposed rat brains, and damage as well of reduced populations of parvalbumin-containing fast-spiking GABAminergic interneurons in various regions of the brain. In conclusion, data from the present study demonstrated the detrimental effects of Gwagwalada landfill leachate on the brain which may be implicated in neuropsychological conditions.
Modelling of Compression Ignition Engine by Soft Computing Techniques (ANFIS-NSGA-II and RSM) to Enhance the Performance Characteristics for Leachate Blends with Nano-additives
Authors: Khan Osama , Parvez Mohd , Kumari Pratibha , Yadav Ashok Kumar , Akram Wasim , Ahmad Shadab , Parvez Samia , Idrisi Mohammad Javed 
Published: May 2023

Abstract
The focus of this study is on the potential of using oils extracted from food waste that ended up in landfills. These waste oils were tested to see how they affected performance and emissions in diesel engines. The study's results are analysed and compared with models created using intelligent hybrid prediction approaches including adaptive neuro-fuzzy inference system (ANFIS), response surface methodology (RSM) - genetic algorithm (GA), and ANFIS - non sorting genetic algorithm (NSGA-II). The analysis takes into account engine load, blend percentage, nanoadditive concentration, and injection pressure, and the desired responses are the thermal efficiency and specific energy consumption of the brakes, as well as the concentrations of carbon monoxide, unburned hydrocarbon, and oxides of nitrogen. Root-mean-square error (RMSE) and the coefficient of determination (R2) were used to assess the predictive power of the model (R2). Comparitevely to AI and the RSM-GA model, the results provided by ANFIS-NSGA-II are superior. This is because it achieved a pareto optimum front of 24.45 kW, 2.76, 159.54 ppm, 4.68 ppm, and 0.020243% for BTE, BSEC, NOX, UBHC, and CO. Combining the precision of ANFIS's prediction with the efficiency of NSGA-optimization II's gives a reliable and thorough evaluation of the engine's settings.
Snowprint: a predictive tool for genetic biosensor discovery
Authors: Stofel Sarah , Ellington Andrew , d'Oelsnitz Simon 
Published: May 2023

Abstract
Bioengineers increasingly rely on ligand-inducible transcription regulators for chemical-responsive control of gene expression, yet the number of regulators available is limited. Novel regulators can be mined from genomes, but an inadequate understanding of their DNA specificity complicates genetic design. Here we present Snowprint, a simple yet powerful bioinformatic tool for predicting regulator:DNA interactions. Benchmarking results demonstrate that Snowprint predictions are significantly similar for 67 out of 147 experimentally validated regulator:operator pairs from organisms across nine phyla and for regulators that span five distinct structural families. We then use Snowprint to design promoters for 33 previously uncharacterized regulators sourced from diverse phylogenies, of which 28 were shown to influence gene expression and 24 produced a >20-fold signal-to-noise ratio. A panel of the newly domesticated regulators were then screened for response to biomanufacturing-relevant compounds, yielding new sensors for a polyketide (olivetolic acid), terpene (geraniol), steroid (ursodiol), and alkaloid (tetrahydropapaverine) with 6-, 3.4-, 2.3-, and 10.7-fold induction ranges. Snowprint represents a unique, generalizable tool that greatly facilitates the discovery of ligand-inducible transcriptional regulators for bioengineering applications.
Age-integrated breast imaging reporting and data system assessment model to improve the accuracy of breast cancer diagnosis
Authors: Deng Jingwen , Shi Manman , Wang Min , Liao Ni , Jia Yan , Yao Feng , Sun Shengrong , Zhang Yimin 
Published: May 2023

Abstract
Purpose
Ultrasonography is one of the most effective methods for diagnosing breast diseases, especially among Asian women. The Breast Imaging Reporting and Data System (BI-RADS) is widely used independently of age in diagnosing breast cancer via ultrasonography. This study aimed to develop a model that integrates age with the BI-RADS lexicon to improve the diagnostic accuracy of ultrasonography in diagnosing breast cancer among the Chinese population.
Methods
This study comprised two cohorts: the training cohort, including 975 women from the Renmin Hospital of Wuhan University, and the validation cohort, including 500 women from the Maternal and Child Health Hospital of Hubei Province. Logistic regression was used to construct a model combining BI-RADS scores with age and to determine the age-based prevalence of breast cancer to predict a cut-off age. The area under the curve (AUC) was used to determine the model’s diagnostic efficacy.
Results
The age with BI-RADS scores model had the best performance compared to the age-only model and BI-RADS scores-only model with an AUC of 0.872 (95% CI: 0.850–0.894, p < 0.001). Moreover, among participants aged < 30 years, the prevalence of breast cancer was lower than the lower limit of the reference range (2%) for the BI-RADS subcategory 4A lesions but within the reference range for BI-RADS category 3 lesions according to the linear regression analysis.
Conclusions
The integrated assessment model based on age and BI-RADS may improve the accuracy of ultrasonography in diagnosing breast lesions. Young patients with BI-RADS subcategory 4A lesions may be excluded from biopsy.
County-Level Artificial Light at Night (ALAN) in the Contiguous U.S. (2012-2019): Spatial Variations, Temporal Trends and Environmental Justice Analyses
Authors: Xiao Qian , Zhou Meng , Lyu Yue , Lu Jiachen , Zhang Kehe , Figueiro Mariana , Wang Jun , Bauer Cici 
Published: May 2023

Abstract
Artificial light at night (ALAN) is a growing environmental exposure with economic, ecological and public health implications. Moreover, previous studies suggested a higher burden of light pollution and related adverse effects in disadvantaged communities. It is critical to characterize the geographic distribution and temporal trend of ALAN and identify associated demographic and socioeconomic factors at the population level to lay the foundation for environmental and public health monitoring and policy making. We used satellite data from the Black Marble suite to characterize ALAN in all counties in contiguous US and reported considerable variations in ALAN spatiotemporal patterns between 2012 and 2019. As expected, ALAN levels were generally higher in metropolitan and coastal areas; however, several rural counties in Texas experienced remarkable increase in ALAN since 2012, while population exposure to ALAN also increased substantially in many metropolitan areas. Importantly, we found that during this period, although the overall ALAN levels in the US declined modestly, the temporal trend of ALAN varied across areas with different racial/ethnic compositions: counties with a higher percentage of racial/ethnic minority groups, particularly Hispanic populations, exhibited significantly less decline. As a result, the disparities in ALAN across racial/ethnic groups exacerbated between 2012 and 2019. In conclusion, our study documented variations in ALAN spatiotemporal patterns across America and identified multiple population correlates of ALAN patterns that warrant future investigations.
Late Quaternary shrews (Mammalia: Soricomorpha) from Priamurye according to data from Koridornaya Cave
Authors: Omelko Valeriya E. , Tiunov Mikhail P. 
Published: May 2023

Abstract
During excavations in Koridornaya Cave (Pompeevskiy Ridge, Jewish Autonomous Oblast, Russia) in 2017–2018, for the first time in Priamurye (Outer Manchuria), numerous bone remains of Late Quaternary mammals were recovered. The purpose of this work was to determine the species list of shrews of that time and to estimate their abundance. In the deposits of Koridornaya Cave, 890 cranial remains of shrews (Soricidae) were found belonging to 11 species from four genera: Sorex, Neomys, Beremendia, and Crocidura. The formation time of the deposits corresponds to the Karginsky interstadial (MIS 3), and the upper part of the deposits contains Holocene (MIS 1) material. Pleistocene communities of shrews of the Pompeevskiy Ridge included modern inhabitants of this territory (S. caecutiens, S. isodon, S. unguiculatus, S. daphaenodon, S. roboratus, S. minutissimus, N. fodiens, and C. lasiura) and S. tundrensis; C. ex gr. suaveolens appeared in the Holocene. Sorex gracillimus, which currently lives here, either was absent in Late Pleistocene communities or was extremely rare. The dominant species in the communities of that time was S. caecutiens, just as in the Southern Sikhote-Alin (Late Pleistocene to Holocene) and in most of modern communities of shrews in the south of the Russian Far East.
Trade-offs and Synergies of Ecosystem Service Bundles in the Luan River Basin, China
Authors: Wang Ye , Wu Zhiyong , Xue Zhongcai , Gao Qiang , Bu Fan , Zhao Jiujia , Yang Yue 
Published: May 2023

Abstract
The relationships between multiple ecosystem services are essential for effective ecosystem management and decision-making. In this study, the Luan River Basin, which is a critical component of the water conservation and ecological environment support area in the Beijing-Tianjin-Hebei region of China, was evaluated for its carbon storage, water yield, soil conservation, water retention, habitat quality, and food supply from 1990 to 2020. Ecosystem service bundles were identified using clustering methods that considered trade-offs and synergies, and the spatiotemporal variations of each bundle type were examined. The results indicated that carbon storage, water yield, soil conservation, water retention, and habitat quality increased after reaching their lowest values in 2000–2005, while food supply showed a significant decline after 2005. Most ecosystem services, except for food supply, were significantly positively correlated with each other, indicating the presence of synergies between them. Based on the ecosystem service value and the area proportion of synergies and trade-offs between ecosystem services, three ecosystem service bundles were identified as "Ecological conservation synergy type," "Food supply trade-off type," and "Ecological balance type." The interconversion between these types of bundles depended on natural background conditions, ecological protection policies, and the degree of agricultural economic development. These findings suggest that ecosystem management in the Luan River Basin should be tailored to specific measures based on the different ecosystem service bundles.
Sibling relationships among firstborn preschoolers with ADHD: a Chinese population- based retrospective cohort study
Authors: Xian Danxia , Zhang Jingyu , Wu Jianbo , Yin Xiaona , Zhang Yan , Qiu Shuangyan , Zhao Yafen , Yang Weikang , Lu Dali 
Published: May 2023

Abstract

Objective:
Attention-Deficit/Hyperactivity Disorder (ADHD) are significantly impaired in social relationships, such as poorer parent-child and peer relationships. However, fewer study to explore sibling relationships in ADHD. Thus, this study is to investigate the quality of sibling relationships among firstborn preschooler with ADHD relative to those without ADHD.
Methods:
This was a population-based cohort study involving 69634 children enrolled in 234 kindergartens in Longhua District of Shenzhen, China. Sibling relationships were assessed with Sibling Inventory of Behavior (SIB) and ADHD symptoms were assessed with the 18-item Swanson, Nolan, and Pelham Rating Scale (SNAP-IV). Logistic regression and ordinal logistic regression models were performed to examine the associations between ADHD symptoms and sibling relationships among preschoolers.
Results:
This study included 8419 first-born children with siblings. Of them, 831 children (9.87%) have ADHD symptoms. Children with ADHD showed significantly higher scores of negative sibling relationships in rivalry, aggression and avoidance, and significantly lower scores of positive sibling relationships in involvement, empathy and teaching compared to non-ADHD group. Rivalry, aggression and child gender (boys) were positively correlated with children with ADHD, while involvement (companionship), teaching and household income were negatively correlated with children with ADHD. In ordinal logistic regression models, rivalry, aggregation, involvement and teaching entered the model, while rivalry and aggregation were risk factors of ADHD, and involvement and teaching were protective factors of ADHD.
Conclusion
It is important to pay more attention to the sibling relationships of children with ADHD.
Validation of the Media Fill method for Cytokine-Induced Killer cells manufacturing process
Authors: FERRERO Ivana , CASTIGLIA Sara , ADAMINI Aloe , MANDESE Alessandra , SISMONDI Francesca , RUSTICHELLI Deborah , LEONE Marco , PINNETTA Giuseppe , GIORDANENGO Lucia , MARESCHI Katia , FAGIOLI Franca 
Published: May 2023

Abstract
Background. Media fills are used to demonstrate the adequacy of the aseptic conditions of a pharmaceutical production process, according to good manufacturing practice (GMP), using a growth medium place of the product solution. For advanced therapy medicinal product (ATMP), where the final product consists of viable cells, the media fill is still a challenge, but represents the starting point for process validation. Methods. The aim of this paper is to describe the media fill test procedure in the context of ATMP manufacturing, in particular of Cytokine-Induced Killer (CIK) cell expansion process under GMP conditions, including quality control tests and environmental monitoring. The media fill test has been designed to cover all the critical steps of the process, including worst cases and deviations. Results. From July 2019 to August 2022, we performed 16 media fill tests. During these years, the media fill protocol has been gradually improved and the worst cases were designed to be closer to reality and occurring cases. Although some deviations occurred, all the media fills performed were compliant. Conclusions. A good media fill design combined with a robust environmental monitoring program provides a high degree of assurance of the microbial safety of ATMPs.
Use of Three-Dimensional Printing Technology for Supporting the Hip Reconstruction Surgery in Paediatric Patients
Authors: Iuppariello Luigi , Calderone Danilo , Casaburi Antonio , Romano Gemma , Guida Pasquale , Clemente Fabrizio 
Published: May 2023

Abstract
The use of three-dimensional (3D) printed patient-specific anatomical models is nowadays a viable strategy for improving surgical outcome in medicine. In adult surgery, 3D printing technology is commonly studied, but its use in paediatric surgery is still under development. This work presents the implementation of 3D printing technology in Orthopaedic department of the paediatric hospital “Santobono-Pausilipon” in Naples by producing 3D printed anatomical models of paediatric patients. The 3D printed models produced were used for the training of the surgical team during the preoperative planning and for carrying out a surgical simulation. The anatomical models are designed in compliance with the current European Medical Devices regulation and following the already existing guidelines in literature. The impact of the 3D printed anatomical models used, a total of seven printed anatomical models based on four patients, is then evaluated throughout a questionnaire proposed to the surgical team, composed by eight paediatric orthopaedic surgeons. Surgeons answered to a total of ten questions, six scale-based questions and four free-text questions. Results obtained from the questionnaires highlighted how 3D printed anatomical models can lead to a better understating of the treated pathologies, carrying relevant improvements in both the surgical team training and the surgical outcome.
Interneuron diversity in the human dorsal striatum
Authors: Garma Leonardo , Harder Lisbeth , Barba-Reyes Juan , Diez-Salguero Monica , Serrano-Pozo Alberto , Hyman Bradley , Munoz-Manchado Ana 
Published: May 2023

Abstract

Deciphering the striatal interneuron diversity is key to understanding the basal ganglia circuit and to untangle the complex neurological and psychiatric diseases affecting this brain structure. We performed snRNA-seq of postmortem human caudate nucleus and putamen samples to elucidate the diversity and abundance of interneuron populations and their transcriptional structure in the human dorsal striatum. We propose a new taxonomy of striatal interneurons with eight main classes and fourteen subclasses and provide their specific markers and some quantitative FISH validation, particularly for a novel PTHLH-expressing population. For the most abundant populations, PTHLH and TAC3, we found matching known mouse interneuron populations based on key functional genes such as ion channels and synaptic receptors. Remarkably, human TAC3 and mouse Th populations share important similarities including the expression of the neuropeptide tachykinin 3. Finally, we were able to integrate other published datasets supporting the generalizability of this new harmonized taxonomy.