bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Immunohistochemical Expression of CD44 and Osteopontin in Colorectal Carcinoma and colorectal adenoma
Authors: El-maksoud Rasha A. Abd , El-Okda Mohamed O. , Zidan Azza H. M. , Wagih Heba M. 
Published: February 2023

Abstract
Background
Colorectal cancer (CRC) is a foremost global health concern and remains one of the major causes of cancer-related morbidity and mortality. It is the third most common cancer in adults after lung cancer and breast cancer worldwide. The theory that cancer originates from a subpopulation of tumor cells, named cancer stem cells (CSC), they have important role of CSC in the initiation and maintenance of the tumor, as well as invasion, metastasis and therapeutic resistance. Among CSC markers, CD44 and OPN are two of the most investigated colorectal CSC markers and their proteins are introduced as the subpopulation with a greater tumorigenicity. This study aiming assessing the immunohistochemical expression of CD44 & OPN in colorectal adenomas & CRCs. And their relation between immunohistochemical expression of CD44 & OPN with tumor differentiation (grading), lympho-vascular invasion, perineural invasion, desmoplasia and TNM stage.
Methods
this is a retrospective descriptive study that included Sixty paraffin embedded blocks from the pathology laboratory, Suez Canal University Hospital. Paraffin blocks included (14 cases of colorectal carcinoma and 18 cases of colorectal adenoma). paraffin blocks reviewed for clinicopathological prognostic factors and stained by CD44 & OPN, monoclonal antibodies by immunohistochemical method.
Results
The CD44 protein was overexpressed in 80% of CRC, while was positive (44.4%) in adenoma this difference was statistically significant. Also, in this study the difference between the expression OPN in CRC and adenomas was statistically insignificant.
Conclusions
CD 44 is highly expressed in large number of CRC (80 of tumors). It is also significantly more expressed in CRC than in adenomas, suggesting a role of CD 44 in CRC tumorigenesis and progression of adenomas into carcinomas. Our study also associated CD 44 overexpression with both late TNM stage and lympho-vascular invasion.
Flaw identification in Plane Linear Piezoelastic Media Using Linear Sampling Method
Authors: Manshadi S.H. Dehghan 
Published: February 2023

Abstract
In this paper, the linear sampling method is introduced for cavity detection in a two dimensional (2D) piezoelastic media using measurement of data on the boundary. This method is categorized as a qualitative approach to image the geometrical features of unknown targets. Although this method has been used in the context of inverse problems such as acoustics, electromagnetism and elastostatic, there is no specific attempt to apply this method to identification of cavities in piezoelastic media. This study emphasizes the implementation of the linear sampling method coupled with the finite element method (FEM). A set of numerical simulations on two-dimensional problems is presented to highlight many effective features of the proposed qualitative identification method.
Engineering Design-controlled Interwoven Multimaterial Scaffolds Combining Hard and Soft Phases
Authors: Cendrero Adrián Martínez , Dehne Tilo , Sittinger Michael , Lantada Andrés Díaz 
Published: February 2023

Abstract
The graded nature of human tissues asks for functional scaffolds with gradients of properties capable of biomimetically repairing injured or deteriorated tissues. These functionally graded tissue engineering scaffolds are of special relevance for promoting the personalized treatment of articular pathologies and osteochondral defects and, despite the multiple research directions explored, no solution is yet perfect. In this study an innovative approach for engineering design-controlled and functionally graded scaffolds is presented. It stands out for combining hard-phase 3D printed lattices and soft-phase PDMS sponges or networks. The developed procedure is illustrated through two case studies: one dealing with the creation of PDMS and PDMS-PLA constructs, as chondral and osteochondral plugs; another focused on the prototyping of personalized PDMS-PLA/resin constructs, as scaffolds for the tissue engineering or repair of the meniscus. Promising potentials for the tissue engineering of complex-shaped and large-size osteochondral and meniscal defects are discussed, and a systematic description of future research directions is included.
Epithelioid Malignant Peripheral Nerve Sheath Tumor of the Bladder and Concomitant Urothelial Carcinoma: A Case Report and Literature Review
Authors: Ozden Sami Berk , Simsekoglu Muhammed Fatih , Sertbudak Ipek , Demirdag Cetin , Gurses Iclal 
Published: February 2023

Abstract
Epitheloid malignant peripheral nerve sheath tumor (eMPNST) of baldder, is a rare entity with devastating features. They are thought to originate from malignant transformation of pre-existing schwannomas of pelvic autonomic nerve plexuses and unlike the conventional MPNST’s they are not found to be in association with neurofibromatosis. In this case report we present the detailed clinical course of a 71-year- old patient with eMPNST of bladder alongside with a review of the literature.
“I was lying on a bed just like the wounded in the war”: A qualitative study explaining the experiences of brain injury patients hospitalized in ICU
Authors: Adineh Mohammad , Molavynejad Shahram , Elahi Nasrin , Jahani Simin , Savaie Mohsen 
Published: February 2023

Abstract
Background: The experiences of patients hospitalized in the ICU can be used as one of the important components to develop and provide a humane and holistic care tailored to the needs and preferences of these patients. Therefore, the present study was conducted to explain the experiences of brain injury patients hospitalized in ICU.
Method: This was a qualitative study using conventional content analysis. The participants included brain injury patients with a history of hospitalization in ICU, and were selected using a purposive sampling method. At least three months after discharge from ICU, face-to-face unstructured in-depth interviews were conducted with the participants to explore their experiences regarding hospitalization in ICU. Finally, the collected data were analyzed based on the content analysis model of Graneheim and Lundman.
Results: Data analysis yielded 238 compact semantic units (initial codes). Based on their similarities and differences, these codes were organized into 22 sub-categories, 6 categories, and 2 themes, including "The suffering body and soul" and "Illumination in the dark".
Conclusion: The results of this study showed that being hospitalized in the ICU is a very difficult and unpleasant experience for brain injury patients due to enduring an immense amount of physical and mental sufferings. Of course, the ICU care and treatment team can moderate this unpleasant experience to some extent by paying careful attention to the physical and mental needs of these patients. The results of the present study can serve as a clinical manual for providing humane and holistic care tailored to the needs and preferences of brain injury patients hospitalized in ICU.
New classification of rheumatoid arthritis based on immune cells and clinical characteristics
Authors: Wang Jiaqian , Zhou Liang , Xue Yuan 
Published: February 2023

Abstract
Background
Rheumatoid arthritis (RA) is a chronic systemic immune disease characterized by joint synovitis, but the specific etiology is unknown, and the characteristic serum diagnostic markers are also lacking.
Methods
First, we obtained the gene expression profile of synovium to evaluate the infiltration of immune cells in synovium, and screened the differentially expressed immune related genes for enrichment analysis. Subsequently, we classified RA into three subtypes by unsupervised clustering of serum gene expression profiles based on immune enrichment scores. Then, the enrichment and clinical characteristics of different subtypes were analyzed. Finally, according to the infiltration of different subtypes of immune cells, diagnostic markers were screened and verified by qRT-PCR.
Results
C1 subtype is related to the increase of neutrophils, CRP and ESR, and joint pain is more significant in patients. C2 subtype is related to the expression of CD8+T cells and Tregs, and patients have mild joint pain symptoms. The RF value of C3 subtype is higher, and the expression of various immune cells is increased. The function of this subtype is enriched in a variety of immune system diseases. T cells CD4, NK cells activated, macrophages M1 and neutrophils are immune cells significantly infiltrated in synovium and serum of RA patients. IFNGR1, TRAC, IFITM1 can be used as diagnostic markers of different subtypes.
Conclusion
In this study, RA patients were divided into different immune molecular subtypes based on gene expression profile, and immune diagnostic markers were screened, which provided a new idea for the diagnosis and treatment of RA.
Leaf area index and light interception relationship with seed yield of indeterminate soybean cultivars under reduced seeding rates
Authors: Ferreira André Sampaio , Zucareli Claudemir , Zeffa Douglas Mariani , Werner Flávia , Shimizu Gabriel Danilo , Junior Alvadi Antonio Balbinot 
Published: February 2023

Abstract
Owing to the recent increase in the cost of germplasm, transgenic royalties, and seed treatments, studies have been conducted to analyze the capacity of modern cultivars to maintain yield under reduced seeding rates (SR). This study elucidated the effect of reduced SR on the evolution of leaf area index (LAI) and light interception by the canopy of soybean cultivars with contrasting branching plasticity and identified the association of these variables with seed yield. Field experiments were conducted in randomized blocks using BRS 1010IPRO (high plasticity) and NS 5959IPRO (medium plasticity) cultivars, with five SRs: 100, 80, 60, 40, and 20% of the recommended SR. The SR reduction did not reduce the seed yield to the point where the LAI and light interception in the reproductive phase were similar to those obtained with the recommended seeding rate. Higher LAI and light interception confer greater potential for reducing the seeding rate in cultivars with higher branching plasticity. The minimum optimal SR (MOSR) for cumulative LAI, Normalized Difference Vegetation Index (NDVI), and intercepted photosynthetic active radiation (IPAR) in the reproductive phase was closer to the MOSR for seed yield than in the vegetative phase or the total crop cycle, indicating “luxury growth” in the vegetative phase at the recommended SRs. Cumulative LAI, NDVI, and IPAR in the reproductive phase had a greater correlation with seed yield than those in the vegetative phase or the total crop cycle. The cumulative NDVI had a higher correlation with seed yield than cumulative LAI and IPAR.
The double product breakpoint as predictor of stress and the correlation between IL-12 and biomarkers in athletes
Authors: Prampero Sérgio Luís , Gonçalves Luis Carlos Oliveira , Mourão Nádia Raquel Dutra Morais , Lopes Jaqueline Santos Silva , França Eduardo Luzia , França Adenilda Honório , Neto Aníbal Monteiro Magalhães 
Published: February 2023

Abstract
The study looked for the impact caused by a treadmill running test on different biomarkers, changes in the cardiovascular system, and correlation between IL-12 and performance. The heatmap showed a correlation of 1.0 (P = 0.00278) between IL-12 and VO2 in the respiratory compensation time and of 0.943 (P = 0 .0167) between pre-exercise IL-12 and the time to reach maximal effort, with the same correlation coefficient and P-value between pre-exercise IL-12 and the distance covered, and pre-exercise IL-12 and the test time. Post-exercise IL-12 presented a correlation coefficient of 0.9 (P = 0.0167) with the heart rate of the respiratory compensation point. A breakpoint for both the double product and heart rate was observed between minutes 2 and 3 of the test, both measurements being more acute than the anaerobic threshold and the respiratory compensation point, and even that breakpoint for systolic blood pressure. The average percentage change in heart rate and the double product obtained the same value (213%). In treadmill running tests with incremental load, the heart rate breakpoint and the double product are the most sensitive and acute markers of the stress level, and the production of IL-12 depends on the intensity and duration of the exercise, which may correlate with performance.
Probabilistic seismic hazard analysis of BPNN predicting PGA
Authors: Guo Xin , Li Hong-Nan , Zhang Hao , Wang Qi , Xu Ji-Ran 
Published: February 2023

Abstract
Probabilistic seismic hazard analysis (PSHA) is recognized as a reasonable method for quantifying seismic threats. Traditionally, the method ignores the effect of focal depth and in which the ground motion prediction equations (GMPEs) are applied to estimate the probability distribution associated with the possible motion levels induced by the site earthquakes, but it is limited by the unclear geological conditions, which makes it difficult to give a uniform equation, and the equation cannot express the nonlinear relationship in geological conditions. Hence, this paper proposes a method to consider the seismic focal depth for the PSHA with the example of California, and use a back propagation neural network (BPNN) to predict peak ground acceleration (PGA) instead of the GMPEs. Firstly, the measured PGA and unknown PGA seismic data applicable to this method are collected separately. Secondly, the unknown PGA data are supplemented by applying the BPNN based on the measured PGA data. Lastly, based on the full-probability equation PSHA considering the focal depth is completed and compared with the current California seismic zoning results. The results show that using the BPNN in the PSHA can ensure computational accuracy and universality, making it more suitable for regions with the unclear geological structures and providing the possibility of adding other parameters to be considered for the influence of PSHA.
Salbutamol and Epinephrine Inhalation as a Conservative Treatment of Transient Tachypnea of Newborn: a Pilot Randomized Controlled Trial
Authors: Noaman Ahmed , Soliman Nahed , Elsallab Shadia , Shabaan Abd Elaziz 
Published: February 2023

Abstract
Objective: To evaluate the efficacy and the safety of inhaled salbutamol and epinephrine in the treatment of TTN.
Design: Double-blinded randomized control pilot study was carried out on 135 near and full-term infants with TTN in the NICU of Mansoura University children's hospital. They were randomly assigned to receive either a single dose of epinephrine, salbutamol, or placebo inhalation. The primary outcome was the level of TTN Downe’s score and the required respiratory support. PaO2 and PaCO2, duration of oxygen therapy, hospital stay, and the adverse effects were considered secondary outcomes. registered at www.clinicaltrials.gov (NCT05006235).
Results: Downe’s score, CPAP requirement, and PaCO2 were significantly less in the salbutamol and epinephrine groups, in addition to a shorter duration of oxygen support and hospital stay with tolerable adverse effects.
Conclusion: Epinephrine or salbutamol inhalation in treatment of TTN is safe, well tolerated, and effective in reduction of the severity of respiratory distress.
Effect of neutral protease overproduction in Bacillus subtilis 168 via site-directed mutation against Meloidogyne incognita infecting eggplant under greenhouse conditions
Authors: Soliman Gaziea M. , El-Sayed Ghada M. , Nour Shaimaa A. , Elkelany Usama S. , Ameen Hoda H. , Hussein walaa 
Published: February 2023

Abstract

Root-knot nematodes (RKN),Meloidogyne incognita, caused significant problems for many important crops. Measuring control with low environmental impact has been required since EU legislation revised pesticide laws for crops. Bacteria-based control methods reduce pollutants and stabilise ecological changes, which makes them promising for controlling plant pathogens. In this study, the derivative ofBacillus subtilis168, termed Bs118, was generated by replacing the native promoter of the extracellular neutral metalloprotease-encoding gene (nprE) with a constitutive promoter of therepUgene responsible for replication of theStaphylococcus aureusplasmid pUB110. As a result, protease production increased to twice that of the wild type. Results revealed that the overproduction of neutral metalloprotease conferred Bs118 high nematocidal activity by inducing 98% mortality in theM. incognitaJ2 in vitro study. Bs118 stated its priority in affecting root-knot nematode reproduction under greenhouse conditions. The soil drench treatment was more promising than root dipping in controllingM. incognitacompared with the untreated control treatment. The same trend happened in the eggplant growth parameters, where Bs118 improved plant health more than Bs168. In conclusion, site-directed mutation via homologous recombination to replace the native promoter with another constitutive one is a promising approach to constructing modified strains with higher protease production that can be employed as an efficient biocontrol agent against root-knot nematodes in addition to the positive impacts on plant growth.
Assembly and comparative analysis of the complete mitochondrial genome of Trigonella foenum-graecum L.
Authors: He Yanfeng , Liu Wenya , Wang Jiuli 
Published: February 2023

Abstract
Background
Trigonella foenum-graecum L. (T. foenum-graecum) is a Leguminosae plant, and the stems, leaves, and seeds of this plant are rich in chemical components that are of high research value. The chloroplast (cp) genome of T. foenum-graecum has been reported, but the mitochondrial (mt) genome remains unexplored.
Results
In this paper, we use second- and third-generation sequencing methods, which have the dual advantage of combining high accuracy and longer read length. The T. foenum-graecum mitochondrial genome was assembled and other analyses such as annotation of the assembled sequences were performed. The results showed that the mitochondrial genome of T. foenum-graecum was 345,604 bp in length and 45.28% in GC content. There are 59 genes, including: 33 protein-coding genes (PCGs), 21 tRNA genes, 4 rRNA genes and 1 pseudo gene. Among them, 11 genes contained introns. Significant AT preferences for codons in the mitochondrial genome of T. foenum-graecum A total of 202 dispersed repetitive sequences, 96 simple repetitive sequences (SSRs) and 19 tandem repetitive sequences were detected. Nucleotide polymorphism analysis counted the variation in each gene, with atp6 being the most notable. Both synteny and phylogenetic analyses showed that T. foenum-graecum was similar to Trifolium pratense, Trifolium meduseum, Trifolium grandiflorum, Trifolium aureum, Medicago truncatula, which are five species of Leguminosae with high similarity. Among them, the highest similarity with Medicago truncatula was 100%. The interspecies non-synonymous substitutions (Ka)/synonymous substitutions (Ks) results showed that 23 Protein-coding genes had Ka/Ks < 1, indicating that these genes would continue to evolve under purifying selection pressure. In addition, 23 homologous sequences were detected in the mitochondrial genome of T. foenum-graecum, and tRNAs were more conserved than PCGs during gene migration.
Conclusions
This paper explores the mitochondrial genome sequence information of T. foenum-graecum and advances the phylogenetic diversity of Leguminosae plants.
Factors affecting lymphedema after neoadjuvant chemotherapy and axillary dissection in female breast cancer patients: A retrospective cohort study based on the Chinese population
Authors: Fu Jianqin , Chen Ruiliang , Yu Yushuai , He Lijuan , Bao Liqun , Lin Zhaodi , Jiang Weijing , Zhang Jie , Song Chuangui , Lin Yanjuan 
Published: February 2023

Abstract
Purpose
Breast cancer-related lymphedema (BCRL) is a common complication in breast cancer survivors. Most BCRL studies have focused on patients receiving adjuvant chemotherapy, and relatively little attention has been paid to BCRL in patients with neoadjuvant chemotherapy (NAC). This study aimed to investigate the risk factors associated with BCRL in Chinese women undergoing NAC and axillary lymph node dissection (ALND).
Methods
In our institution, this cohort study collected data from 336 women with clinically node-positive breast cancer who received NAC and ALND surgery from 2015 to 2020. The BCRL was assessed by objective measurement of limb circumference. Multivariate logistic regression was used to determine risk factors for BCRL, including clinical, demographic and lifestyle-related characteristics.
Results
The cumulative incidence of BCRL within 2.5 years was 43.75%. The factors independently associated with BCRL were radiotherapy (versus no radiotherapy; hazard ratio [HR]=1.611; P=0.020), NAC for 105 days or shorter (versus NAC for 105-143 days; HR=0.471; P=0.020), >15 lymph nodes removed (versus ≤15 lymph nodes removed; HR=1.593; P=0.036 ), drainage for 20-29 days (versus drainage for 10-19 days; HR=1.568; P=0.028), and sleeping biased toward affected arm (versus sleeping biased toward healthy arm; HR=2.033; P=0.019).
Conclusion
This study identified several risk factors for BCRL in breast cancer patients after NAC and ALND. Patients with one or more of these factors should be monitored carefully for early detection and intervention. Further research is needed to investigate the effects of drainage time and sleep position on the development of BCRL.
Active site tuning based on pseudo-binary alloys for low-temperature acetylene semihydrogenation
Authors: Ma Jiamin , Xing Feilong , Shimizu Ken-ichi , Furukawa Shinya 
Published: February 2023

Abstract
The development of an efficient catalytic system for low-temperature acetylene semihydrogenation using nonnoble metals is important for the cost-effective production of polymer-grade pure ethylene. However, it remains challenging owing to the intrinsic low activity. Herein, we report a flexibly tunable catalyst design concept based on a pseudo-binary alloy, which enabled a remarkable enhancement in the catalytic activity, selectivity, and durability of a Ni-based material. A series of (Ni1 − xCux)3Ga/TiO2 catalysts exhibiting L12-type pseudo-binary alloy structures with various Cu contents (x = 0.2, 0.25, 0.33, 0.5, 0.6, and 0.75) were prepared for active site tuning. The optimal catalyst, (Ni0.8Cu0.2)3Ga/TiO2, exhibited outstandingly high catalytic activity among reported 3d transition metal-based systems and excellent ethylene selectivity (96%) and long-term stability (100 h) with near full conversion even at 150°C. A mechanistic study revealed that Ni2Cu hollow sites on the (111) surface weakened the strong adsorption of acetylene and vinyl adsorbate, which significantly accelerated the hydrogenation process and inhibited undesired ethane formation.