bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics analysis reveals that CirA in Aeromonas hydrophila is involved in nutrient uptake
Authors: Song Qingli , Zhang Lishan , Wang Guibin , Song Huanhuan , Zhang Shuangziying , Yao Jindong , Lin Xiangmin 
Published: January 2023

AbstractThe colicin I receptor (CirA) is a well‐studied outer membrane protein that has been reported to play important roles in antibiotic resistance, virulence, and iron homeostasis, although its exact physiological roles require further investigation. In this study, differentially expressed proteins between the ΔahcirA and wild‐type (WT) strains of Aeromonas hydrophila were compared using quantitative proteomics. Bioinformatics analysis revealed that the expression of peptide, histidine, and arginine ATP‐binding cassette (ABC) transporter system‐related proteins was significantly higher in the ΔahcirA strain. Subsequent growth assays revealed that ΔahcirA grew slower than the WT strain in nutrient‐limited medium when supplemented with dipeptide, histidine, and arginine as the carbon source. Far‐western blot analysis further confirmed that AhCirA can directly bind to histidine/arginine and dipeptide small‐molecule substrates in addition to their periplasmic‐binding proteins, AhDppA and AhHisJ, respectively. These results indicate that AhCirA may play an important role in the uptake of amino acids and peptides as a channel‐forming porin while also directly interacting with ABC transporters to transport nutrient substances into the plasma membrane. Overall, this study demonstrates that AhCirA is a multifunctional protein in A. hydrophila and extends our understanding of known nutrient transport mechanisms among bacteria.
Those who Wear the Crown Wield the Knife: the Brutality of Recent Takeover Reshuffles
Authors: Allen Nicholas 
Published: January 2023

AbstractRecent ‘takeover’ Prime Ministers have tended to conduct relatively brutal Cabinet reshuffles immediately after taking office. Liz Truss dismissed and moved large numbers of ministers after becoming Prime Minister in September 2022, and Rishi Sunak did the same after succeeding Truss in October 2022. This article locates the tendency towards brutalism in the broader context of British Cabinet reshuffles. It compares the scale of takeover reshuffles with other types of reshuffles and demonstrates how incoming takeover Prime Ministers in the twenty‐first century have reconstructed their Cabinets more extensively than their predecessors. The article argues that the use of all‐member ballots in party leadership elections has been a key driver of brutalism, and it further identifies several reasons why future Prime Ministers might want to resist the tendency.
Ethanol stability in unpreserved refrigerated antemortem blood
Authors: Kosecki Patrick Allan , Canonico Erika , Abbott Lori 
Published: January 2023

AbstractEthanol stability in preserved antemortem blood has been widely studied since it is a common practice in cases involving suspected impaired driving to collect antemortem blood in evacuated blood tubes containing sodium fluoride. In some situations, antemortem blood is submitted to a forensic laboratory for ethanol analysis in evacuated blood tubes that contain only an anticoagulant. There has been limited research on ethanol stability in antemortem blood stored without a preservative. On two occasions, antemortem blood was collected from five ethanol‐free individuals into 6‐ml Vacutainer® tubes containing only 10.8 mg potassium EDTA. The blood tubes were spiked with ethanol to approximately either 0.08 or 0.15 g/dl. Dual‐FID headspace gas chromatography was used to analyze 58 blood tubes, 29 from each session, for ethanol 1 day after sample collection and again after 1 year of refrigerated storage (~4°C). Statistically significant decreases in ethanol were detected at the 0.05 level of significance. Mean decreases in ethanol after 1 year of storage for the 0.08 and 0.15 g/dl samples were 0.013 and 0.010 g/dl, respectively. The mean ethanol decrease across all tubes was 0.012 g/dl. The range of decreases for the 58 blood tubes was 0.003–0.018 g/dl. The mean ethanol decreases measured in this unpreserved antemortem blood are comparable in magnitude to those previously observed in antemortem blood containing sodium fluoride after 1 year of refrigerated storage. Ethanol did not increase in the antemortem blood samples despite the absence of sodium fluoride.
The effect of food intake during hemodialysis on blood pressure: A nonrandomized experimental trial
Authors: Avci Melek , Arikan Fatma 
Published: January 2023

AbstractBackgroundIntradialytic hypotension (IDH) is one of the most important intradialytic complications and is thought to be associated with intradialytic food intake. Allowing intradialytic feeding is still unclear. This study aimed to determine the relationship between IDH and intradialytic food intake.MethodsA nonrandomized experimental study was conducted to determine the effect of intradialytic food intake on blood pressure (BP) in hemodialysis patients. Each patient was assessed twice using an ambulatory BP monitor with and without intradialytic food intake.ResultsThe study was completed with 54 patients. Intradialytic hypotension developed in 40 patients (74.1%) during the food intake session, while intradialytic hypotension developed in 22 participants (40%) in the no‐food session. Repeated BP readings showed that eating 2 h or more after the start of hemodialysis significantly reduced BP.ConclusionsIntradialytic food intake affects the development of IDH. Patients who ate during hemodialysis had a trend of higher of IDH than those who ate nothing during hemodialysis. Eating during dialysis is not recommended.
Effect of febuxostat on adverse events and mortality in gout in Taiwan: An interrupted time series analysis
Authors: Tsai Ping‐Han , Kuo Chang‐Fu , Liu Jia‐Rou , Li Pei‐Ru , See Lai‐Chu 
Published: January 2023

AbstractObjectivesTo evaluate the influence of febuxostat on adverse events and mortality in gout.MethodsWe retrospectively enrolled patients with newly diagnosed gout and prescribed urate‐lowering therapy between 2006 and 2017 from the Taiwan National Health Insurance Database. These patients were divided into 2 groups: with and without comorbidities (n = 294 847 and 194 539). An interrupted time series analysis with adjustments for demographics, comorbidities, and comedication by propensity score‐based stabilized weights was used to compare the trend of adverse events and mortality before vs after febuxostat was introduced in 2012.ResultsThe proportion of febuxostat use gradually increased from 0% in 2012 to 30% in those with comorbidities and 10% in those without comorbidities in 2017. Allopurinol use decreased from 30% in 2012 to 10% in 2017. The slope of the 1‐year incidence rate of Stevens–Johnson syndrome (SJS) or toxic epidermal necrolysis (TEN) (per 10 000 patients) significantly reduced after 2012 in those with and without comorbidities (−0.375 per quarter, P = .015 and −.253 per quarter, P = .049). The slope of the 3‐year incidence rate of acute myocardial infarction (AMI) (per 1000 patients), percutaneous coronary intervention (PCI) (per 1000 patients), and all‐cause mortality (per 100 patients) significantly increased after 2012 in those with comorbidities (+0.207 per quarter, P = .013; +.389 per quarter, P = .002; +.103 per quarter, P = .001).ConclusionsFebuxostat may reduce SJS and TEN in all gout patients but increase AMI, PCI, and all‐cause mortality in gout patients with comorbidities.
Awe culture and corporate social responsibility: Evidence from China
Authors: Yan Chao , Wang Jiaxin , Wang Zhi , Chan Kam C. 
Published: January 2023

AbstractBy proxying ‘awe culture’ (i.e., reverence for life and ethical behaviour) with regional induced abortion rates, we examine the impact of awe culture on corporate social responsibility (CSR) in a sample of Chinese firms. We find that firms located in areas with higher induced abortion rates spend less funds on CSR activities and obtain lower CSR scores. The findings remain intact after an array of robustness tests. Further analysis shows that the effect of awe culture on CSR is more pronounced in areas with weaker law enforcement and where the local government emphasises economic growth targets. However, the effect becomes insignificant when firms are well‐represented by top executives with overseas experience, foreign directors, and a high proportion of female board members. The significance of the effect also diminishes for non‐state‐owned firms, and firms with higher institutional ownership and higher cash holdings. Moreover, the lack of awe culture attenuates the positive impact of CSR on firm value. Overall, we document that awe culture, as an informal institution, shapes CSR behaviours.
Understanding the differential effect of local socio‐economic conditions on the relation between prescription opioid supply and drug overdose deaths in US counties
Authors: Fink David S. , Keyes Katherine M. , Branas Charles , Cerdá Magdalena , Gruenwald Paul , Hasin Deborah 
Published: January 2023

AbstractBackground and aimsBoth local socio‐economic conditions and prescription opioid supply are associated with drug overdose deaths, which exhibit substantial geographical heterogeneity across the United States. We measured whether the associations of prescription opioid supply with drug overdose deaths vary by local socio‐economic conditions.DesignEcological county‐level study, including 3109 US counties between 2006 and 2019 (n = 43 526 county‐years) using annual mortality data.SettingUnited States.CasesA total of 711 447 drug overdose deaths.MeasurementsWe modeled overdose counts using Bayesian hierarchical Poisson models, estimating associations between four types of drug overdose deaths (deaths involving any drugs, any opioid, prescription opioids only and heroin), prescription opioid supply and five socio‐economic indicators: unemployment, poverty rate, income inequality, Rey index (components include mean household income, % high school graduates, % blue‐collar workers and unemployment rate), and American human development index (HDI; an indicator of community wellbeing).FindingsDrug overdose deaths and all substance‐specific overdose deaths were higher in counties with higher income inequality [adjusted odds ratios (aORs) = 1.09–1.13], Rey index (aORs = 1.15–1.21) and prescription opioid supply (aORs = 1.14–1.21), and lower in counties with higher HDI scores (aORs = 0.75–0.92). Poverty rate, income inequality and HDI scores were found to modify the effect of prescription opioid supply on heroin overdose deaths. The plot of the interactions showed that when disadvantage is high, increasing prescription opioid supply does not increase heroin overdose deaths. The less disadvantage there is, indicated by lower poverty rates, higher HDI scores and lower income inequality, the greater the effect of increasing prescription opioid supply relative to population size on heroin overdose deaths in US counties.ConclusionsIn the United States, prescription opioid supply is associated with higher drug overdose deaths; associations are stronger in counties with less disadvantage and less income inequality, but only for heroin overdose deaths.
An intensive longitudinal examination of topiramate treatment for alcohol use disorder: a secondary analysis of data from a randomized controlled trial
Authors: Votaw Victoria R. , Witkiewitz Katie , Van Horn M. Lee , Crist Richard C. , Pond Timothy , Kranzler Henry R. 
Published: January 2023

AbstractBackground and AimsPrevious findings have been equivocal as to whether a single‐nucleotide polymorphism (rs2832407) inGRIK1, which encodes a glutamate receptor subunit, moderates the effects of topiramate treatment for drinking reduction. We leveraged intensive longitudinal data to provide greater precision and allow an examination of intermediate outcomes addressing this question. We used data from a randomized controlled trial (RCT) to test the hypotheses that topiramate treatment reduces daily heavy drinking, desire to drink and positive alcohol expectancies and that these effects are stronger in rs2832407*C‐allele homozygotes.DesignSecondary data analysis of a randomized controlled trial.SettingUniversity of Pennsylvania Treatment Research Center in the United States.Participants/CasesParticipants were 164 individuals (70.1% male, mean age = 51.42, 36.0% rs2832407*C‐allele homozygotes) who sought to reduce or stop drinking.Intervention and ComparatorParticipants were assigned to medication (topiramate or placebo), with stratification by genotype group (CC versus AA/AC) and treatment goal (reduce versus abstain).MeasurementsDuring the 12‐week treatment period, participants completed daily interactive voice response (IVR) surveys.FindingsOn any given day during treatment, participants who received topiramate had lower odds of IVR‐reported heavy drinking [odds ratio (OR) = 0.259,b (standard error, SE) = −1.351 (0.334),P < 0.001] and lower levels of desire to drink [b (SE) = −0.323 (0.122),P = 0.009] and positive alcohol expectancies [b (SE) = −0.347 (0.138),P = 0.013] than those who received placebo. Participants who received topiramate also reported greater reductions in positive alcohol expectancies during the first 2 weeks of treatment than those who received placebo [b (SE) = −0.028 (0.008),P = 0.001], but topiramate did not impact the daily rate of change in heavy drinking or desire to drink. Genotype did not moderate the effects of topiramate on any outcomes examined (P > 0.05).ConclusionsTopiramate is an effective medication for individuals seeking to reduce heavy drinking. The effects are not moderated by the single‐nucleotide polymorphism rs2832407.
The association between maternal and paternal substance use and child substance use, internalizing and externalizing problems: a systematic review and meta‐analysis
Authors: McGovern Ruth , Bogowicz Paul , Meader Nick , Kaner Eileen , Alderson Hayley , Craig Dawn , Geijer‐Simpson Emma , Jackson Katherine , Muir Cassey , Salonen Domna , Smart Deborah , Newham James J. 
Published: January 2023

AbstractAimsThere is substantial evidence showing an association between parental substance use and child substance use and/or mental health problems. Most research focuses upon maternal substance use, with the influence of paternal substance use often being overlooked. We aimed to investigate the differential effects of maternal and paternal substance use upon children aged 0–18 years.MethodsWe used systematic review methods to identify observational studies examining the association between either maternal or paternal substance use and child substance use and/or mental health problems. The odds ratio (OR) effect measure was used, for ease of computation. We used a random‐effects model with the inverse variance method to meta‐analyse the findings from eligible studies.ResultsWe included 17 unique studies with a total of 47 374 child participants. Maternal and paternal substance use were both associated with increased odds of child any drug use [OR = 2.09; 95% confidence interval (CI) = 1.53, 2.86; n = 12 349 participants; three studies and OR = 2.86; 95% CI = 1.25, 6.54; n = 5692 participants; three studies, respectively], child alcohol problem use (OR = 2.16; 95% CI = 1.73, 2.71; n = 7339 participants; four studies and OR = 1.70; 95% CI = 1.36, 2.12; n = 14 219 participants; six studies), child externalizing problems (OR = 1.81; 95% CI = 1.01, 3.22; n = 1748 participants; three studies and OR = 1.60; 95% CI = 1.18, 2.17; n = 2508 participants; six studies) and child internalizing problems (OR = 1.60; 95% CI = 1.25, 2.06; n = 1748 participants; three studies and OR = 1.42; 95% CI = 1.12, 1.81; n = 2248 participants; five studies). Child any alcohol use was associated with maternal substance use only (OR = 2.26; 95% CI = 1.08, 4.70; n = 28 691 participants; five studies).ConclusionsBoth maternal and paternal substance use are associated with child substance use and mental health problems.
Newborns requiring resuscitation: Two thirds have heart rate ≥100 beats/minute in the first minute after birth
Authors: Kibsgaard Amalie , Ersdal Hege , Kvaløy Jan Terje , Eilevstjønn Joar , Rettedal Siren 
Published: January 2023

AbstractAimThe aim was to study the prevalence of bradycardia at birth in newborns requiring positive pressure ventilation (PPV), distribution of first measured heart rate (HR), changes in HR before start of PPV and HR response to PPV.MethodsA population‐based study including newborns ≥30 weeks' gestation receiving PPV at birth. HR was captured immediately after birth and continuously throughout resuscitation using the dry‐electrode ECG device NeoBeat. Time of birth was registered in the Liveborn app. Provision of PPV was captured by video.ResultsWe included 98 newborns receiving PPV at birth. Among newborns with HR measured within 60 s after birth, median (quartiles) first HR was 112 (84, 149) bpm recorded 19 (14, 37) s after birth, of which 33% had first HR <100 and 10% had first HR <60 bpm respectively. First HR was widely distributed. Median HR at start PPV 69 s after birth was 129 bpm. In newborns with an initial low HR, HR typically remained low for 20 s of PPV before increasing rapidly over the next 20–30 s.ConclusionsFirst measured HR was ≥100 bpm in two thirds of newborns receiving PPV. In bradycardic infants, HR did not increase until after 20 s of PPV.
The CaSR/TRPV4 coupling mediates pro‐inflammatory macrophage function
Authors: Chen Xiongying , Lu Wei , Lu Cheng , Zhang Luyun , Xu Feng , Dong Hui 
Published: January 2023

AbstractAimAlthough calcium‐sensing receptor (CaSR) and transient receptor potential vanilloid 4 (TRPV4) channels are functionally expressed on macrophages, it is unclear if they work coordinately to mediate macrophage function. The present study investigates whether CaSR couples to TRPV4 channels and mediates macrophage polarization via Ca2+ signaling.MethodsThe role of CaSR/TRPV4/Ca2+ signaling was assessed in lipopolysaccharide (LPS)‐treated peritoneal macrophages (PMs) from wild‐type (WT) and TRPV4 knockout (TRPV4 KO) mice. The expression and function of CaSR and TRPV4 in PMs were analyzed by immunofluorescence and digital Ca2+ imaging. The correlation factors of M1 polarization, CCR7, IL‐1β, and TNFα were detected using q‐PCR, western blot, and ELISA.ResultsWe found that PMs expressed CaSR and TRPV4, and CaSR activation‐induced marked Ca2+ signaling predominately through extracellular Ca2+ entry, which was inhibited by selective pharmacological blockers of CaSR and TRPV4 channels. The CaSR activation‐induced Ca2+ signaling was significantly attenuated in PMs from TRPV4 KO mice compared to those from WT mice. Moreover, the CaSR activation‐induced Ca2+ entry via TRPV4 channels was inhibited by blocking phospholipases A2 (PLA2)/cytochromeP450 (CYP450) and phospholipase C (PLC)/Protein kinase C (PKC) pathways. Finally, CaSR activation promoted the expression and release of M1‐associated cytokines IL‐1β and TNFɑ, which were attenuated in PMs from TRPV4 KO mice.ConclusionWe reveal a novel coupling of the CaSR and TRPV4 channels via PLA2/CYP450 and PLC/PKC pathways, promoting a Ca2+‐dependent M1 macrophage polarization. Modulation of this coupling and downstream pathways may become a potential strategy for the prevention/treatment of immune‐related disease.
Dietary linseed supplementation improves meat quality and flavor of sheep by altering muscle fiber characteristics and antioxidant capacity
Authors: Hou Yanru , Liu Chang , Su Lin , Zhao Lihua , Yang Zhihao , Bai Yanping , Dou Lu , Yao Duo , Jin Ye 
Published: January 2023

AbstractLinseed as a common oil crop that has been implicated in regulating the meat quality of animals. The aim of this investigation was to establish the effects of dietary linseed supplementation on carcass traits, meat quality, muscle fiber characteristics, volatile compounds and antioxidant capacity of sheep. Twenty‐four Sunit sheep were distributed into 2 groups with different diet: control (CO) fed a standard diet and linseed (LS, 8% of extruded linseed). The results showed that linseed not only increased M. longissimus thoracis (LT) increased the loin‐eye area but also decreased pH24h, L* and shear force. Meanwhile, linseed increased mRNA expression of MyHCI and MyHCIIx and decreased in cross‐sectional area (CSA) and muscle fiber diameter. In addition, linseed altered the composition of meat volatile flavor compounds, such as Z‐10‐Pentadecen‐1‐ol, pentanal, 2‐Octenal, (E)‐, decanal, butane, and 2‐heptanone. Moreover, linseed increased total antioxidative capacity (T‐AOC) activity, the mRNA expression of glutathione peroxidase (GSH‐Px) and catalase (CAT), decreased malondialdehyde (MDA) content. Overall, the results suggest that linseed is an effective feed additive in improving meat quality and flavor. The underlying mechanisms for its effectiveness may be partly due to a change in muscle fiber characteristics and antioxidant capacity.
Knowledge base for social capital's role in scaling social impact: A bibliometric analysis
Authors: Mohiuddin Md. Fazla , Yasin Ida Md. , Latiff Ahmed R. A. 
Published: January 2023

AbstractSocial capital and scaling social impact are two of the most important concepts within social entrepreneurship and social enterprise research. However, what role social capital plays in scaling social impact is less understood and academic literatures on the connection of these two crucial concepts are fragmented and scattered. To fill this research gap, we have conducted a bibliometric review to inform academics and researchers the salient agents in the field and categorize the conceptual structure of the knowledge base. Using science mapping techniques, we have analyzed 1549 relevant documents from SCOPUS that are published between 1986 and 2021. The primary objective of this review is to uncover the size, growth trajectory, and geographic distribution of literatures related to social capital's role in scaling social impact. Additionally, we have also analyzed key publishing journals, authors, and documents to map the intellectual structure and research fronts of the domain. The review shows that there is a reasonable accumulation of scientific knowledge concerning social capital's role in scaling social impact in the last three decades. Another key finding is that, some of the keywords identified within the research fronts are closely aligned with a number of UN‐SDG goals. This is the first study to systematically map social capital's role in scaling social impact literature with the help of a science mapping tool called bibliometric analysis. This work provides a clear overview of the evolution of the research field and presents potentially important research gaps that require further attention.
Model Uncertainty Quantification in Cox Regression
Authors: García-Donato Gonzalo , Cabras Stefano , Castellanos María Eugenia 
Published: January 2023

Abstract
We consider covariate selection and the ensuing model uncertainty aspects in the context of Cox regression. The perspective we take is probabilistic, and we handle it within a Bayesian framework. One of the critical elements in variable/model selection is choosing a suitable prior for model parameters. Here, we derive the so-called conventional prior approach and propose a comprehensive implementation that results in an automatic procedure. Our simulation studies and real applications show improvements over existing literature. For the sake of reproducibility but also for its intrinsic interest for practitioners, a web application requiring minimum statistical knowledge implements the proposed approach.