bluuebunny/crossref_metadata_2025_split huggingface dataset:
How did home care nurses support COVID‐19 patients in Japan? A qualitative study
Authors: Shirouchi Mana , Sumikawa Yuka , Yoshioka‐Maeda Kyoko , Yamamoto‐Mitani Noriko 
Published: January 2025

AbstractAimHome care nurses support patients with COVID‐19 with mild to moderate symptoms at home due to the lack of community‐based support. Little is known about how nurses initiated and maintained support for patients with COVID‐19. This study explored the experiences of home care nurses in supporting patients with COVID‐19 at home.MethodsUsing snowball sampling, 21 home care nurses participated in semi‐structured interviews conducted either in person or online between September 2021 and February 2023. A qualitative study using a grounded theory approach was conducted.ResultsHome care nurses faced a situation where “suffering patients are overflowing” due to inadequate support from public health centers and designated hospitals. Despite their anxiety, they acted as a “flexible safety net by quickly compensating for uncertain support systems” for patients, families, and local health workers. They “built tentative support systems immediately” and “updated tentative support systems, own knowledge, skills, and mindset.” Home care nurses also “rushed to patient/family” to provide timely support and “avoided preventable death.”ConclusionsHome care nurses act as a flexible safety net, preventing patient deaths by quickly compensating for uncertain support systems before and after initiating care. Their efforts complemented the inadequacies of traditional infectious disease control systems, typically managed by public health centers. Strengthening secondary and tertiary prevention systems is essential for home care nurses to save lives while delivering individualized care during the COVID‐19 pandemic.
Making Zoning Relevant for Business Students: An Interactive Exercise in Selecting a Business Location
Authors: Grow Lara 
Published: January 2025

AbstractThis article describes an in‐class exercise that can be used when teaching zoning law to a Real Estate Law or Legal Environment of Business undergraduate course. The exercise requires students to utilize Salt Lake City's zoning code to locate the best site for a restaurant chain given certain locational and operational requirements, and to research the likelihood of a variance request. The exercise is an effective means for instructors to connect an increasingly relevant area of the law to a practical business objective.
Regulation of neural stem cells by innervating neurons
Authors: Dittmann Nicole Leanne , Chen Lauren , Voronova Anastassia 
Published: January 2025

AbstractThe adult central nervous system (CNS) hosts several niches, in which the neural stem and precursor cells (NPCs) reside. The subventricular zone (SVZ) lines the lateral brain ventricles and the subgranular zone (SGZ) is located in the dentate gyrus of the hippocampus. SVZ and SGZ NPCs replace neurons and glia in the homeostatic as well as diseased or injured states. Recently, NPCs have been found to express neurotransmitter receptors, respond to electrical stimulation and interact with neurons, suggesting that neuron‐NPC communication is an emerging critical regulator of NPC biology. In this review, we discuss reports that demonstrate neuronal innervation and control of the neurogenic niches. We discuss the role of innervating neurons in regulating NPC fates, such as activation, proliferation, and differentiation. Our review focuses primarily on the innervation of the SVZ niche by the following neuronal types: glutamatergic, GABAergic projection and interneurons, cholinergic, dopaminergic, serotonergic, neuropeptidergic, nitrergic, and noradrenergic. We also discuss the origins of SVZ niche innervating neurons, such as striatum, cortex, basal ganglia, raphe nuclei, substantia nigra and ventral tegmental area, hypothalamus, and locus coeruleus. Our review highlights the various roles of innervating neurons in SVZ NPC fates in a spatiotemporal manner and emphasizes a need for future investigation into the impact of neuronal innervation on NPC gliogenesis.image
Successful pain control of lymphangioma‐suspected lesions in pregnancy with acetaminophen and opioids: A case report and literature review
Authors: Kawatani Haruna , Shibata Takashi , Kotsuji Fumikazu , Kato Hiroki , Nishikawa Shigeki , Nakago Satoshi 
Published: January 2025

AbstractLymphangioma causing pain during pregnancy is not widely reported, but surgical treatment was performed in each of the reported cases. In our patient, lesions arose in the retroperitoneal cavity and caused intense pain from 17 weeks of gestation. However, surgical treatment was considered difficult because of the location of the lesion. The maximum dose of acetaminophen was administered, but pain relief remained unsatisfactory, and there were concerns about possible side effects of long‐term use of high‐dose acetaminophen on the fetus. Following attempts to control pain and reduce the acetaminophen dose, adding opioids was effective in controlling the pain and minimizing both dosages as much as possible. The patient was able to continue her pregnancy to term, avoiding highly invasive surgery during pregnancy. The combination of acetaminophen and opioids is reported here as an alternative treatment option to surgical procedures for intense pain caused by lesions such as lymphangioma in pregnancy.
Epidemiology and outcomes of alpha‐1 antitrypsin deficiency in Sweden 2002–2020: A population‐based cohort study of 2286 individuals
Authors: Wahlin Staffan , Widman Linnea , Hagström Hannes 
Published: January 2025

AbstractObjectiveTo estimate the incidence, prevalence, and outcomes of patients with diagnosed alpha‐1‐antitrypsin deficiency (AATD) in Sweden, 2002–2020.Study design and settingThe Swedish National Patient Registry was utilized to identify patients with a first diagnosis of AATD between 2002 and 2020. Each patient was matched with up to 10 comparators from the general population. AATD incidence and prevalence were estimated. Causes of death and rates of mortality, transplantation, lung disease, liver cirrhosis, and previous neonatal cholestasis were estimated.ResultsThe incidence rate of AATD was 1.83 (95% confidence interval [CI] 1.58–2.11) per 100,000 person‐years and the total prevalence was 21.04 (95%CI = 20.17–21.94) per 100,000 persons at the end of 2020. Mortality was 3.55 times higher (95%CI = 3.15–3.99) for patients with AATD. Rates of liver—(hazard ratio [HR] = 22.95, 95%CI = 12.61–41.75), lung—(HR = 12.09, 95%CI = 8.87–16.47), and cardiovascular (HR = 1.90, 95%CI = 1.45–2.90) related death were higher in patients with AATD. The cumulative incidence after 10 years of follow‐up was 1.69% (95%CI = 1.15–2.41) for liver transplantation and 4.14% (95%CI = 3.20–5.26) for lung transplantation. About 20% of patients were estimated to be alive without lung disease or liver cirrhosis 20 years after an AATD diagnosis. Neonatal cholestasis codes were found in 3.0% of AATD patients and 0.5% of comparators (odds ratio 6.28, 95%CI = 3.81–10.36).ConclusionsIn this population‐based cohort study on AATD in Sweden, an increasing incidence was observed, and significantly higher rates of death from liver, lung, and cardiovascular causes compared to the general population were found. Only a minority of diagnosed AATD patients were estimated to be free of liver cirrhosis and lung disease after 20 years.
Simultaneous Estimation of Stable Parameters for Multiple Autoregressive Processes From Datasets of Nonuniform Sizes
Authors: Lederer Johannes , Sachs Rainer von 
Published: January 2025

ABSTRACTWe develop a finite‐sample theory for estimating the coefficients and for the prediction of multiple stable autoregressive processes that (i) share an unknown lag order but (ii) can differ in their sample sizes. Our technique is based on penalisation similar to hierarchical, overlapping group‐Lasso but requires a new mathematical set‐up to accommodate (i) and (ii). The set‐up differs from existing work considerably, for example, in that we estimate the common lag order directly from the data rather than using extrinsic criteria. We prove that the estimated autoregressive processes enjoy stability, and we establish rates for both the estimation and prediction error that can outmatch the known rates in our setting. Our insights on lag selection and stability are also of interest in the case of individual autoregressive processes.
Resilience assessment of urban rail transit stations considering disturbance and time‐varying passenger flow
Authors: Liu Xiaowei , Chen Jinqu , Du Bo , Yan Xu , Peng Qiyuan , Shen Jun 
Published: January 2025

AbstractUnlike most urban rail transit (URT) resilience studies on URT lines or networks under major disturbances, this paper focuses on the resilience assessment of URT stations under high‐frequency daily disturbances with minor impacts. A resilience assessment metric with different resilience levels is proposed, which is calculated based on multiple criteria, including the number of delayed passengers, degree of congestion, economic loss from service suppliers’ perspective, extra in‐station travel time, extra walking distance, and extra waiting time from passengers’ perspective. A two‐stage passenger flow redistribution model is developed with stage one focusing on route adjustment under disturbance, while stage two determining the walking path within the disrupted station. A case study of Simaqiao Station in the Chengdu subway network in China is conducted. The numerical results indicate that this station demonstrates strong resilience in most scenarios, although it faces challenges under certain identified disturbances.
Sudden borders in the north: Regional resilience and nationalism in the Torne Valley during COVID‐19
Authors: Gaber Katrina 
Published: January 2025

AbstractThe Torne Valley used to be described as one of the most peaceful and most integrated border areas in the world. This changed radically during the COVID‐19 pandemic when the border between Sweden and Finland precipitously became materialised through the physical installation of a border fence in 2020. With the help of rich empirical data collected through interviews, this paper examines the social struggle between border region resilience and everyday nationalism in the Sweden–Finland borderland area, the Torne valley, in the context of the COVID‐19 pandemic. The aim of the paper is to shed light on the complex processes of identity creation and the relevance of regional and national identities in the north. The analysis examines the processes through which regional and national identities are constructed, the meaning of these identities in this context, and provides novel insights on how the pandemic affected social ties in the north.
Insertion of the β‐ketoacyl‐CoA synthase MdKCS2 promoter segment causes wax biosynthesis difference in apple peel
Authors: Zhang Lizhi , Yin Shaobo , Wang Mingqian , Liu Zhi , Lv Tianxing , Wang Yanan , Wang Aide , Tan Dongmei , Ji Yinglin 
Published: January 2025

Summary
Cuticular wax is essential for fruit to maintain moisture. Although the wax content of peel surface in apple (Malus spp.) varies, the detailed molecular mechanism remains unclear.
Here, we identified the β‐ketoacyl‐CoA synthase 2 (MdKCS2) differentially expressed between apple peel with low and high wax content by integrating bulked segregant analysis‐sequencing and RNA‐seq. We found that a 63‐bp insertion in the MdKCS2 promoter was the primary reason for apple peel with low wax content. The 63‐bp insertion reduced MdKCS2 promoter activity and enhanced the DNA binding with the suppressor MdDOF4.6, decreasing wax biosynthesis by reducing C24 very‐long‐chain fatty acid (VLCFA).
ECERIFERUM 2 (MdCER2) was co‐expressed with MdKCS2 and suppressed by MdMYB56, MdbHLH137 and MdDOF4.6, further decreasing C29 alkane content in apple peel with low C24 VLCFA content.
Overall, MdKCS2 and MdCER2 are coordinately involved in the wax production of apple peel surface.
Soil microbes influence the ecology and evolution of plant plasticity
Authors: Bolin Lana G. 
Published: January 2025

Summary
Stress often induces plant trait plasticity, and microbial communities also alter plant traits. Therefore, it is unclear how much plasticity results from direct plant responses to stress vs indirect responses due to stress‐induced changes in soil microbial communities.
To test how microbes and microbial community responses to stress affect the ecology and potentially the evolution of plant plasticity, I grew plants in four stress environments (salt, herbicide, herbivory, and no stress) with microbes that had responded to these same environments or with sterile inoculant.
Plants delayed flowering under stress only when inoculated with live microbial communities, and this plasticity was maladaptive. However, microbial communities responded to stress in ways that accelerated flowering across all environments. Microbes also affected the expression of genetic variation for plant flowering time and specific leaf area, as well as genetic variation for plasticity of both traits, and disrupted a positive genetic correlation for plasticity in response to herbicide and herbivory stress, suggesting that microbes may affect the pace of plant evolution.
Together, these results highlight an important role for soil microbes in plant plastic responses to stress and suggest that microbes may alter the evolution of plant plasticity.
Circadian Proteomics Reassesses the Temporal Regulation of Metabolic Rhythms by Chlamydomonas Clock
Authors: Jadhav Dinesh Balasaheb , Roy Sougata 
Published: January 2025

ABSTRACTCircadian clocks execute temporal regulation of metabolism by modulating the timely expression of genes. Clock regulation of mRNA synthesis was envisioned as the primary driver of these daily rhythms. mRNA oscillations often do not concur with the downstream protein oscillations, revealing the importance to study protein oscillations. Chlamydomonas reinhardtii is a well‐studied miniature plant model. We quantitatively probed the Chlamydomonas proteome for two subsequent circadian cycles using high throughput SWATH‐DIA mass spectrometry. We quantified > 1000 proteins, half of which demonstrate circadian rhythms. Among these rhythmic proteins, > 90% peak around subjective midday or midnight. We uncovered key enzymes involved in Box C/D pathway, amino acid biosynthesis, fatty acid (FA) biosynthesis and peroxisomal β‐oxidation of FAs are driven by the clock, which were undocumented from earlier transcriptomic studies. Proteins associated with key biological processes such as photosynthesis, redox, carbon fixation, glycolysis and TCA cycle show extreme temporal regulation. We conclude that circadian proteomics is required to complement transcriptomic studies to understand the complex clock regulation of organismal biology. We believe our study will not only refine and enrich the evaluation of temporal metabolic processes in C. reinhardtii but also provide a novel understanding of clock regulation across species.
The Vacuolar Inositol Transporter BvINT1;1 Contributes to Raffinose Biosynthesis and Reactive Oxygen Species Scavenging During Cold Stress in Sugar Beet
Authors: Berg Johannes , Rodrigues Cristina Martins , Scheid Claire , Pirrotte Yana , Picco Cristiana , Scholz‐Starke Joachim , Zierer Wolfgang , Czarnecki Olaf , Hackenberg Dieter , Ludewig Frank , Koch Wolfgang , Neuhaus H. Ekkehard , Müdsam Christina , Pommerrenig Benjamin , Keller Isabel 
Published: January 2025

ABSTRACTDespite a high sucrose accumulation in its taproot vacuoles, sugar beet (Beta vulgaris subsp. vulgaris) is sensitive to freezing. Earlier, a taproot‐specific accumulation of raffinose was shown to have beneficial effects on the freezing tolerance of the plant. However, synthesis of raffinose and other oligosaccharides of the raffinose family depends on the availability of myo‐inositol. Since inositol and inositol‐metabolising enzymes reside in different organelles, functional inositol metabolism and raffinose synthesis depend on inositol transporters. We identified five homologues of putative inositol transporters in the sugar beet genome, two of which, BvINT1;1 and BvINT1;2, are localised at the tonoplast. Among these, only the transcript of BvINT1;1 is highly upregulated in sugar beet taproots under cold. BvINT1;1 exhibits a high transport specificity for inositol and sugar beet mutants lacking functional BvINT1;1 contain increased inositol levels, likely accumulating in the vacuole, and decreased raffinose contents under cold treatment. Due to the quenching capacity of raffinose for Reactive Oxygen Species (ROS), which accumulate under cold stress, bvint1;1 sugar beet plants show increased expression of both, ROS marker genes and detoxifying enzymes. Based on these findings, we conclude that the vacuolar inositol transporter BvINT1;1 is contributing to ROS‐homoeostasis in the cold metabolism of sugar beet.
Deep metabolomics revealed trajectories of jasmonate signaling‐mediated primary metabolism in Arabidopsis upon Spodoptera litura herbivory
Authors: Kundu Anish , Bera Paramita , Mishra Shruti , Vadassery Jyothilakshmi 
Published: January 2025

AbstractPlants defend against chewing herbivores by up‐regulating jasmonic acid (JA) signaling, which activates downstream signaling cascades and produces numerous secondary metabolites that act as defense molecules against the herbivores. Although secondary metabolism always remains a focus of research, primary metabolism is also reported to be realigned upon herbivory. However, JA signaling‐mediated modulation of primary metabolites and their metabolic pathways in plants are mostly unexplored. Here, we applied gas chromatography–mass spectrometry‐based untargeted metabolomics aided with computational statistical frameworks on wild type Arabidopsis, mutants of active JA receptor (i.e., CORONATINE‐INSENSITIVE 1, COI1‐1) and downstream transcription factor (i.e., MYC2) to navigate the JA signaling‐mediated primary metabolism alterations during herbivory. Pathway and metabolite's chemical class enrichment analysis revealed JA signaling is crucial for constitutive as well as herbivore‐induced primary metabolism and topology of their interaction networks. JA signaling majorly modulated alterations of sugars, amino acids and related metabolites. Herbivory‐mediated sugar depletion and induction of methionine for aliphatic glucosinolates are also dependent on JA signaling. Taken together, our results demonstrate trails of JA signaling‐mediated primary metabolic alterations associated with herbivory.
The Nexus Between Flush Toilet Use and Off‐Farm Performance of Rural Households
Authors: Li Junpeng , Liu Xiao , Meng Xianghai , Zhang Wenguang 
Published: January 2025

ABSTRACTThis study examines the impact of flush toilet use on farmers' off‐farm performance. We analyse data from 2012, 2014 and 2016 China Labour‐force Dynamics Survey (CLDS) by employing the two‐stage residual inclusion (TSRI) model to address the endogeneity issues associated with flush toilet use. The results show that flush toilet use not only significantly increases the probability of farmers' participation in off‐farm work by 3.5% but also improves their off‐farm income by 2100 yuan/capita/year. Disaggregated analyses reveal that farmers characterised as female, well‐educated and aware of water pollution benefit the most from using flush toilets in terms of off‐farm work participation and off‐farm income. Our mediation analysis confirms that farmers' use of flush toilet enhances their health conditions, which links them to improved off‐farm performance. Our findings highlight a practical pathway to enhance farmers' economic welfare by demonstrating the significance of flush toilets in improving the performance of rural households' off‐farm.
The effects of compassionate care on oral health outcomes: A scoping review
Authors: Mendes Mariana Sarmet Smiderle , Ferreira Camila Lopes , Jardini Maria Aparecida Neves , Childs Christopher Andrew , Marchini Leonardo 
Published: January 2025

AbstractPurposeTo access and synthesize the existing literature about the effects of compassionate care on oral health outcomes.MethodsA scoping review was performed following Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines to identify articles from six electronic databases: MEDLINE via the PubMed interface, Embase, Age‐Line, Scopus, CINAHL, and Cochrane.ResultsAmong 30 studies reporting on compassion in dental treatment, only two reported on the effects of compassionate care on oral health outcomes. One study is cross‐sectional and evaluated oral health outcomes in children, while the other is a cohort study evaluating oral health outcomes in older adults. Each study used different tools to assess oral health outcomes. However, both studies used the Jefferson Scale of Physician Empathy (JSPE) to assess empathy among dental students, dentists, or dental hygienists.ConclusionThe retrieved studies used different methodologies and had discrepant results. More clinical studies investigating the effects of compassionate care on the outcomes of dental treatment are necessary.
A paired study comparing mononuclear cell collection using a new in‐line system with an off‐line extracorporeal photopheresis apheresis system
Authors: Monsalvo Silvia , Quintana Claudia , Mosquera Claudia , Bocanegra Ana , Aguilar Myriam , León Ana García‐de , de Miguel Carlos , Forés Rafael , Laporta Rosalía , Bautista Guiomar , Fernández Roberto , Almonacid Carlos , Duarte Rafael F. , Bueno José L. 
Published: January 2025

AbstractBackgroundExtracorporeal photopheresis (ECP) product characteristics are not well established. The aim of this study was to compare mononuclear cells (MNCs) collection using the new Amicus blue (AB) In‐line ECP system to our standard Off‐line ECP system using the Optia apheresis device and the MacoGenic G2 inactivation system (OM).Study Design and MethodsWe assessed the ECP products and procedure parameters, patient characteristics, and adverse events for both AB and OM systems in paired patients. Comparisons were made with t‐test for paired samples.ResultsThirteen patients underwent 15 double, paired procedures using both ECP protocols and processing the same blood volume of 4000 mL. Total MNC collected in the product were 51.6 × 108 (95% CI 30.0–73.1) and 42.2 × 108 (95% CI 22.4–62.0) for the AB and OM, respectively (not significant). Both products were also similar regarding volume, MNC concentration, purity, and hematocrit. However, total platelet count (×1011) was significantly lower in the AB products: 0.25 (95% CI 0.15–0.36) versus 1.2 (95% CI 0.9–1.5). The new AB system reduced significantly also the time invested and anticoagulant used per procedure compared with OM, albeit with similar collection efficiency and percentage of MNC captured per procedure. Hypocalcemia was the commonest adverse event with both systems, but it was not severe.ConclusionsThe new AB system collected MNC products comparable to our current experience with OM, although in a significantly shorter time, with a reduced use of anticoagulant and lower contamination with platelets, which are all valuable advantages of the new system.