bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polydopamine (PDA) coatings with endothelial vascular growth factor (VEGF) immobilization inhibiting neointimal formation post zinc (Zn) wire implantation in rat aortas
Authors: Fu Jiayin , Zhu Qiongjun , Chen Zhezhe , Zhao Jing , Wu Shaofei , Zhao Meng , Xu Shihui , Lai Dongwu , Fu Guosheng , Zhang Wenbin 
Published: June 2023

Abstract

BackgroundBioresorbable stents are designed to provide temporary mechanical support to the coronary arteries and then slowly degradein vivoto avoid chronic inflammation. Zinc (Zn) is a promising material for bioresorbable stents; However, it can cause inflammation and neointimal formation after being implanted into blood vessels.MethodsTo improve biocompatibility of Zn, we first coated it with polydopamine (PDA), followed by immobilization of endothelial vascular growth factor (VEGF) onto the PDA coatings. Adhesion, proliferation, and phenotype maintenance of endothelial cells (ECs) on the coated Zn were evaluated. Then, a wire aortic implantation model in rats mimicking endovascular stent implantation in humans was used to assess vascular responses to the coated Zn wires in vivo. Thrombosis in aortas post Zn wire implantation, degradation of Zn wires in vivo, neointimal formation surrounding Zn wires, and macrophage infiltration and extracellular matrix (ECM) remodeling in the neointimas were examined.ResultsIn vitrodata showed that PDA-coated Zn encouraged EC adhesion, spreading, proliferation, and phenotype maintenance on its surfaces. VEGF functionalization on PDA coatings further enhanced the biocompatibility of Zn to ECs. Implantation of PDA-coated Zn wires into rat aortas didn’t cause thrombosis and showed a faster blood flow than pure Zn or the Zn wires coated with VEGF alone. In addition, the PDA coating didn’t affect the degradation of Zn wiresin vivo. Besides, the PDA-coated Zn wires reduced neointimal formation, increased EC coverage, decreased macrophage infiltration, and declined aggrecan accumulation in ECM. VEGF immobilization onto PDA coatings didn’t cause thrombosis and affect Zn degradationin vivoas well, and further increased the endothelization percentage as compared to PDA coating alone, thus resulting in thinner neointimas.ConclusionThese results indicate that PDA coatings with VEGF immobilization would be a promising approach to functionalize Zn surfaces to increase biocompatibility, reduce inflammation, and inhibit neointimal formation after Zn implantationin vivo.
Evaluation of port competitiveness along China’s “Belt and Road” based on the entropy-TOPSIS method
Authors: Wang Jie , Mo Lilian , Ma Zhu 
Published: June 2023

Abstract
Based on the literature on the factors influencing port competitiveness, this paper evaluates the competitiveness of Chinese ports along the Belt and Road based on Porter's diamond model. The evaluation system includes four primary indicators, including port infrastructure, port operation scale, port hinterland economic level and port development potential, and thirteen secondary indicators. Then, based on the entropy-TOPSIS method, an evaluation model of port competitiveness is constructed. Finally, 15 seaports along China's "Belt and Road" are used to evaluate competitiveness, and a comprehensive ranking of port competitiveness is obtained. The advantages and shortcomings of each port are pointed out according to the evaluation results to provide a reference for the long-term development of the competitiveness of ports along the "Belt and Road" in China.
The developmental origins of health and disease and intergenerational inheritance: a scoping review of multigenerational cohort studies
Authors: Tan Jie , Zhang Zifan , Yan Lijing , Xu Xiaolin 
Published: June 2023

Abstract
Background
Epidemiologic research has increasingly acknowledged the importance of developmental origins of health and disease and suggests that prior exposures can be transferred across generations. Understanding the intergenerational inheritance has profound implications for developing public health interventions to prevent diseases. Multigenerational cohorts are crucial to verify the above-mentioned issues among human subjects. We carried out this scoping review aims to map existing literature to summarize multigenerational cohort studies' characteristics, issues, and implications and hence provide evidence to the developmental origins of health and disease hypothesis and intergenerational inheritance.
Methods
This study followed Arksey and O’Malley’s five-stage scoping review framework. We adopted a three-step search strategy to identify multigenerational cohorts comprehensively, searching PubMed, EMBASE, and Web of Science databases from the inception of each dataset to June 20th, 2022, to retrieve relevant articles. We aim to include all the existing multigenerational cohorts. Data of included cohorts were extracted using a standardized tool, to form a descriptive analysis and a thematic summary.
Results
After screening, 28 unique multigenerational cohort studies were identified. We classified all studies into four types: population-based cohort extended three generation cohort, birth cohort extended three generation cohort, three generation cohort, and integrated birth and three generation cohort. Most cohorts (n = 15, 53%) were categorized as birth cohort extended three-generation studies. The sample size of included cohorts varied from 41 to 167,729. The study duration ranged from two years to 31 years. Most cohorts had comprehensive data collection schemes. Almost all cohorts had common exposures, including socioeconomic factors, lifestyle, and grandparents’ and parents’ health and risk behaviors over the life course. These studies usually investigated intergenerational inheritance of diseases as the outcomes, most frequently, obesity, child health, and cardiovascular diseases.
Conclusions
Most multigenerational studies aim to disentangle genetic, lifestyle and environmental contributions to the developmental origins of health and disease across generations. We call for more research on large multigenerational well-characterized cohorts, up to four or even more generations, and more studies from low-and middle-income countries.
Immune and stromal transcriptional patterns that influence the outcome of classic Hodgkin Lymphoma
Authors: Garcia Juan F , Menendez Victoria , Solorzano Jose L , Garcia-Cosio Monica , Alonso-Alonso Ruth , Rodriguez Marta , Cereceda Laura , Fernandez Sara , Diaz Eva , Montalban Carlos , Estevez Monica , Piris Miguel Angel 
Published: June 2023

Abstract
Classic Hodgkin lymphoma (cHL) is characterized by a rich immune microenvironment as the main tumor component. It involves a broad range of cell populations, which are largely unexplored, even though they are known to be essential for growth and survival of Hodgkin and Reed–Sternberg cells. We profiled the gene expression of 25 FFPE cHL samples using NanoString technology and resolved their microenvironment compositions using cell-deconvolution tools, thereby generating patient-specific signatures. The results confirm individual immune fingerprints and recognize multiple clusters enriched in refractory patients, highlighting the relevance of: 1) the composition of immune cells and their functional status, including myeloid cell populations (M1-like, M2-like, plasmacytoid dendritic cells, myeloid-derived suppressor cells, etc.), CD4-positive T cells (exhausted, regulatory, Th17, etc.), cytotoxic CD8 T and natural killer cells; 2) the balance between inflammatory signatures (such as IL6, TNF, IFN-γ/TGF-β) and MHC-I/MHC-II molecules; and 3) several cells, pathways and genes related to the stroma and extracellular matrix remodeling. A validation model combining relevant immune and stromal signatures identifies patients with unfavorable outcomes, producing the same results in an independent cHL series. Our results reveal the heterogeneity of immune responses among patients, confirm previous findings, and identify new functional phenotypes of prognostic and predictive utility.
Silver carp experience metabolic and behavioral changes when exposed to water from the Chicago Area Waterway; implications for upstream movement
Authors: Schneider Amy , Esbaugh Andrew , Suski Cory 
Published: June 2023

Abstract

One of the hallmarks of invasive species is their propensity to spread. Removing an invasive species after establishment is virtually impossible, and so considerable effort is invested in preventing the range expansion of invaders. Silver carp were discovered in the Mississippi River in 1981 and have spread throughout the basin. Despite their propensity to expand, the ‘leading edge’ in the Illinois River has stalled south of Chicago, and has remained stable for a decade. Studies have suggested that pollutants in the Chicago Area Waterway System (CAWS) may be contributing to the lack of upstream movement, but this hypothesis has not been tested. This study used a laboratory setting to quantify the role of pollutants in deterring upstream movement of silver carp within the CAWS. For this, water was collected from the CAWS near the upstream edge of the distribution and transported to a fish culture facility. Silver carp and one native species were exposed to CAWS water, and activity, behavior, avoidance and metabolic rates were quantified. Results showed that silver carp experience an elevated metabolic cost in CAWS water, along with reductions in swimming behavior. Together, results suggest a role for components of CAWS water at deterring range expansion.
An in vitro model for Reactive Astrogliosis: Differentiated U-87 MG cells as biochemical analogues of reactive astrocytes
Authors: Arora Tania , Prashar Vikash , Singh Randeep , Sharma Arti , Changotra Harish , Parkash Jyoti 
Published: June 2023

Abstract
Reactive astrogliosis (RA) is a complex pathological condition where astrocytes undergo specific biochemical changes to attain hypertrophy and hyperplasia. RA is a mechanism being observed in various neurodegenerative disorders (ND) such as Alzheimer's disease (AD), Parkinson's Disease (PD) and traumatic brain Injury (TBI). Various models have been designed to understand the mechanism of activation as well as to determine therapeutics to reverse or attenuate the condition for major neurological disorders but the study is hampered due to the lack of a suitable in vitro model. In this study, we propose a new in vitro model by using U-87 MG (human glioblastoma cells) cells which are differentiated by using retinoic acid and transformed by using proinflammatory and anti-inflammatory cytokines to generate the A1/A2 phenotype. The model was further validated by using comparative expression analysis of marker proteins and profiling of a panel of miRNA. The purpose of this study was to understand the molecular switch mechanism for the interconversion of these cells that can be used in the development of new therapeutic interventions for multiple neurological conditions. Since multi targeted drugs are on the rise, it will be beneficial to design therapies that could direct both neuronal and astrocytic milieu towards neuroprotection.
Folic acid grafted mixed polymeric micelles as a targeted delivery strategy for tamoxifen citrate in treatment of breast cancer.
Authors: Nasr mohamed , Hashem Fahima , Teiama Mohammed , Tantawy Norhan , Abdelmoniem Raghda 
Published: June 2023

Abstract
The objective of this study was to develop folic acid (FA) grafted mixed polymeric micelles loaded with Tamoxifen citrate (TMXC) to enhance its antitumor activity in breast tissues. The conjugated FA-P123 micelles were prepared using carbonyl diimidazole cross-linker chemistry and confirmed using FTIR and 1HNMR. The micelles were examined for encapsulation efficiency, particle size, surface charge, in vitro release study, cytotoxic effect, and cellular uptake by a breast cancer cell line. The conjugated TMXC-loaded micelle exhibited a nanoparticle size of 35.01 ±1.20 nm, a surface charge of - 20.50 ± 0.95 mV, entrapped 87.83 ±5.10 % and released 67.58 ± 2.47 % of TMXC after 36 h. The conjugated micelles exhibited a significantly higher cellular uptake of TMXC by MCF-7 cell line and improved in vitro cytotoxicity by 2.48 folds compared to the TMXC-loaded unconjugated micelles. The results of in vivo studies indicated that TMXC-loaded FA-P123/P84 has a potential antitumor activity, as revealed by a significant reduction of tumor volume in tumor-bearing mice compared to TMXC-loaded unconjugated micelles. In conclusion, the obtained results suggested that conjugated FA-P123/P84 micelles could be an encouraging carrier for the treatment of breast cancer with TMXC.
Data-driven modeling for online fault pre-warning in thermal power plant using incremental Gaussian Mixture Regression
Authors: Jin Shengxiang , Si Fengqi , Dong Yunshan , Ren Shaojun 
Published: June 2023

Abstract
This study introduces a data-driven model for online fault pre-warning in thermal power plant using incremental Gaussian mixture regression. To tackle the issue of outdated parameters in existing fault pre-warning models, this study puts forth an incremental Gaussian mixture regression that leverages the merging of Gaussian components to reconstruct the model and enable online modeling. Due to its criticality, a forgetting factor is introduced during the merging process to efficiently manage the weight allocation between present and historical patterns, thereby guaranteeing the model’s accuracy. The results of the sine function case demonstrate that the IGMR model exhibits excellent pattern control performance and modeling efficiency. Furthermore, the IGMR model is employed to forecast parameter alterations in pulverizer blockages with mode switching, and experimental validation indicates that IGMR precisely anticipates parameter changes following mode switching. Compared to on-site solutions, the pre-warning of coal blockage has a clear advantage in advance warning.
Acanthopanax senticosus ameliorates steatohepatitis through HNF4 alpha pathway activation in mice
Authors: Kawano Yutaka , Tanaka Maki , Satoh Yasushi , Sugino Shigekazu , Suzuki Jun , Fujishima Masaki , Okumura Eri , Takekoshi Hideo , Uehara Osamu , Sugita Shintaro , Abiko Yoshihiro , Tomonari Tetsu , Tanaka Hironori , Takeda Hidekatsu , Takayama Tetsuji 
Published: June 2023

Abstract
Non-alcoholic fatty liver disease is a common liver disease worldwide, and is associated with dysregulation of lipid metabolism, leading to inflammation and fibrosis. Acanthopanax senticosus Harms (ASH) is widely used in traditional medicine as an adaptogen food. We examined the effect of ASH on steatohepatitis using a high-fat diet mouse model. Mice were fed a choline-deficient, L-amino acid-defined, high-fat diet with ASH extract (ASHE). After 6 weeks, liver RNA transcriptome sequencing (RNA-Seq) was performed, followed by Ingenuity Pathway Analysis (IPA). Our findings revealed that mice fed a high-fat diet with 5 % ASHE exhibited significantly reduced liver steatosis. These mice also demonstrated alleviated inflammation and reduced fibrosis in the liver. IPA of RNA-Seq indicated that hepatocyte nuclear factor 4 alpha (HNF4 alpha), a transcription factor, was the activated upstream regulator (P-value 0.00155, z score=2.413) in the liver of ASHE-fed mice. Adenosine triphosphate binding cassette transporter 8 and carboxylesterase 2, downstream targets of HNF4 alpha pathway, were upregulated. Finally, ASHE-treated HepG2 cells exposed to palmitate exhibited significantly decreased lipid droplet contents. Our study provides that ASHE can activate HNF4 alpha pathway and promote fat secretion from hepatocytes, thereby serving as a prophylactic treatment for steatohepatitis in mice.
Anticancer potential of furanocoumarins and flavonoids of Heracleum persicum fruit
Authors: Dehnoee Ali , Kalbasi Roozbeh Javad , Tavakoli Saeed , Zangeneh Mohammad Mahdi , Zangeneh Akram , Delnavazi Mohammad-Reza 
Published: June 2023

Abstract
Heracleum persicum Desf. ex Fischer (Umbelliferae) is a herbaceous perennial plant distributed in Iran and Turkey. The aromatic fruits of this plant are commonly used as food additive, carminative, antiseptic and tonic. The present study was designed to isolate non-volatile constituents of H. persicum fruits and evaluate their anti-tumor potentials against different cancer cells. Phytochemical analysis using chromatography on silica gel and Sephadex LH-20 columns resulted in the isolation of phellopterin (1), angelicin (2), pimpinellin (3), bergapten (4), isopimpinellin (5) and xanthotoxin (6) from dichloromethane fraction along with apterin (7), isorhamnetin 3-O-glucoside (8), isorhamnetin 3-O-rutinoside (narcissin) (9), and quercetin 3-O-rutinoside (rutin) from n-butanol fraction of H. persicum fruits extract. 1H-NMR and 13C-NMR spectral analyses were applied to characterize the chemical structures. In MTT assay all of the tested compounds demonstrated preferential cytotoxic activity against cancer cells (IC50; 40–370 µg/mL) in comparison with HUVEC normal cells (IC50; > 1000 µg/mL). Among the compounds phellopterin (1) showed the highest anti-tumor activity toward U-266, SK-MM-1 and RPMI-8226 multiple myeloma cells with IC50 values of 44.3 ± 1.4, 69.1 ± 1.2 and 85.7 ± 1.8 µg/mL, respectively.
Prevalence of bottle feeding practice and its associated factors among mothers of infant less than six months at Addis Ababa public health centers, Addis Ababa, Ethiopia, 2022
Authors: Birhan Gedifw , Mohammed Shikur , Tenia² Sisay , Belyneh Abebe , Alem Amanual , Alemante² Amlaku , Mengistu Belete 
Published: June 2023

Abstract

Introduction
Bottle feeding practice should be avoided due to its influence on optimal breastfeeding and its effect associated with morbidity and mortality resulting from diarrheal disease, infant and child illness, and malnutrition. This study is aimed to assess the prevalence of bottle-feeding practice and its associated factors among mothers of infants less than six months in Addis Ababa.
Methods
An institution-based cross-sectional study was conducted among 456 mothers who had infants less than six months in Addis Ababa public health facilities. Data were collected through interview, entered using Epi info version 7.1 and exported to statistical package for social science version 26 for analysis. Variables with p-value < 0.2 in bivariable binary logistic regression analysis were entered to multivariable logistic regression analysis. Finally, variables with p-value < 0.05 in multivariable logistic regression were taken as significant factor.
Results
The overall prevalence of bottle-feeding practice in this study was 39%. Being male infant (AOR = 0.589; 95%CI: 0.381, 0.911), infant hospitalization (AOR = 2.114; 95%CI 1.246, 3.588), cesarean section delivery (AOR = 2.817; 95%CI: 1.764, 4.496), late initiation of breastfeeding (AOR = 5.526; 95%CI: 3.121, 9.786) and not having information on breast feeding (AOR = 4.13; 95%CI 2.21, 7.73) were statistically significant factors associated with bottle feeding practice.
Conclusion
The magnitude of bottle-feeding practice is high in Addis Ababa. Being male infants, infants hospitalized, cesarean section delivery, late initiation of breastfeeding and not having information on breast feeding were significant factors for bottle feeding practice. Greater efforts to promote breastfeeding should be targeted towards women with sub-optimal breastfeed.
An elevated level of Interleukin-17A in a Senegalese malaria cohort is associated with rs8193038 IL-17A genetic variant
Authors: THIAM Fatou , DIOP Gora , COULONGES Cedric , DERBOIS Celine , THIAM Alassane , DIOUARA Abou Abdallah Malick , MBAYE Mame Ndew , DIOP Mamadou , NGUER Cheikh Momar , DIEYE Yakhya , MBENGUE Babacar , ZAGURY Jean-Francois , DELEUZE Jean-Francois , DIEYE Alioune 
Published: June 2023

Abstract
Malaria infection is a multifactorial disease partly modulated by host immuno-genetic factors. Recent evidence has demonstrated the importance of Interleukin-17 family proinflammatory cytokines and their genetic variants in host immunity. However, limited knowledge exists about their role in parasitic infections such as malaria. We aimed to investigate IL-17A serum levels in patients with severe and uncomplicated malaria and the gene polymorphism’s influence on the IL-17A serum levels. 125 severe (SM) and uncomplicated (UM) malaria patients and 48 free malaria controls were enrolled in this research. IL-17A serum levels were measured with ELISA. PCR and DNA sequencing were used to assess host genetic polymorphisms in IL-17A. We performed a multivariate regression to estimate the impact of human IL-17A variants on IL-17A serum level and malaria outcome. Elevated serum IL-17A levels accompanied by increased parasitemia were found in SM patients compared to UM and controls (P < 0.0001). Also, the IL-17A levels were lower in SM patients who were deceased than in those who survived. In addition, the minor allele frequencies (MAF) of two IL-17A polymorphisms (rs3819024 and rs3748067) were more prevalent in SM patients than UM patients indicating an essential role in SM. Interestingly, the heterozygous rs8193038 AG genotype was significantly associated with higher levels of IL-17A than the homozygous wild type (AA). According to our results, it can be concluded that the IL-17A gene rs8193038 polymorphism significantly affects IL-17A gene expression. Our results fill a gap in the implication of IL-17A gene polymorphisms on the cytokine level in a malaria cohort. IL-17A gene polymorphisms also may influence cytokine production in response to Plasmodium infections and may contribute to the hyperinflammatory responses during severe malaria outcomes.
A new step-wise surgical technique of knapsack-like uterine compression sutures for intractable postpartum hemorrhage in cesarean section
Authors: Han Lei , Zhang Baolin , Yin Hongmei , Xu Huishu , Pang Yiwei , Zhang Xianghui , Zhai Qingliang , Liu Xiaofeng , Wang Yanlin , Zhang Caiying , Liu Yanni , Xuemei Chen 
Published: June 2023

Abstract
Objectives
We aimed to explore the effectiveness and safety of a new type of uterine compression suture, the step-wise surgical technique of knapsack-like sutures for treating intractable postpartum hemorrhage (PPH) caused by uterine atony and placenta factors in cesarean section.
Methods
The step-wise surgical technique of knapsack-like sutures was established on the basis of the artful combination of vertical strap-like sutures and an annular suture-ligation technique. This novel surgical technique was applied to 34 patients diagnosed with PPH during cesarean section due to severe uterine atony and placental factors in our department. The hemostatic effects, clinical outcomes and follow-up visit results were all reviewed and analyzed.
Results
This new uterine compression suture successfully stopped bleeding in 33 patients, and the effective rate was 97.06%. Only 1 patient failed and was changed to use bilateral uterine arterial embolization and internal iliac artery embolization. The follow-up visits indicated that 33 patients restored menstruation except for 1 who was diagnosed with amenorrhea. The gynecological ultrasound tests of all the patients suggested good uterine involutions, and they had no obvious complaints such as hypogastralgia.
Conclusions
This step-wise surgical technique of knapsack-like uterine compression sutures can compress the uterus completely. It is a technique that can conserve the uterus and fertility function without special equipment in caesarean section for PPH, with the characteristics of being safe, simple and stable (3S) with rapid surgery, reliable hemostasis and resident doctor to operation (3R).