bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myocardial infarction and viral triggers: what do we know by now?
Authors: Caldeira Daniel , Nogueira-Garcia Beatriz 
Published: February 2023

AbstractMyocardial infarction (MI) is an acute clinical manifestation ischaemic heart disease, which is the leading cause of death worldwide. Infections also have an important burden worldwide, with lower respiratory infections being the worldwide leading cause of death due to communicable diseases. The relationship of MI with viral respiratory infections (including influenza and SARS-CoV-2) as a trigger has been well documented with significant associations. These infections can lead to Type 1 MI, where inflammation and vascular dysfunction, as well as the increased prothrombotic environment lead to atherothrombosis. Type 2 MI may also occur due to an imbalance of oxygen/blood supply and myocardial demand (hypoxaemia, fever, and tachycardia). The data from randomized controlled trials showing a potential benefit of influenza vaccination in coronary artery disease patients should not be ignored. This can be considered a further argument for the association of viral infections (influenza in particular) and MI.
Wolbachia and Spiroplasma endosymbionts in the Anurida maritima (Collembola) species group
Authors: Timmermans Martijn J T N , Prabha Hari , Kett Stephen 
Published: February 2023

Abstract
A large proportion of arthropods carry maternally-inherited endosymbiotic bacteria with which they have developed close relationships. Some of these endosymbionts are selfish genetic elements and manipulate their hosts’ reproduction to their own advantage, for example via cytoplasmic incompatibility (CI) or male killing. Here we report that the sexually reproducing collembolan Anurida maritima carries two endosymbionts (Wolbachia and Spiroplasma) and provide genome sequences for both bacteria. Phylogenomic analyses indicate that the Wolbachia belong to the A supergroup and that the Spiroplasma are sister to the Citri-Chrysopicola-Mirum lineage. Anurida maritima is considered a species group and consists of at least two distinct genetic lineages. We show that both lineages carry both endosymbionts. No homologues of the Spiroplasma male-killing gene SpAID were observed within our datasets. Homologues of the male-killing associated wmk and the CI inducing cifA and cifB prophage genes were detected in the Wolbachia genome. Phylogenetic analyses placed the cif genes in the poorly characterized Type V clade. The cifA and cifB gene sequences of the two Anurida lineages are identical. It therefore seems unlikely that the genetic divergence within the species group stems from cifA and cifB induced CI. Laboratory controlled genetic crosses and sex ratio studies will be needed to reveal any potential effect of the two endosymbionts on A. maritima’s reproduction.
“I Lay Awake at Night”: Latino Family Caregivers’ Experiences Covering Out-of-Pocket Costs When Caring for Someone Living With Dementia
Authors: Mage Susanna , Benton Donna , Gonzalez Alexander , Zaragoza Gabby , Wilber Kate , Tucker-Seeley Reginald , Meyer Kylie 
Published: February 2023

Abstract

Background and Objectives
The financial burden of caregiving has received less research attention than physical and emotional costs. This is especially true for underserved ethnic minorities. Financial strain affects mental and physical health and is unequally distributed across caregivers of different races and ethnicities. Although caregivers overall spend, on average, one quarter of their income on caregiving, Latino caregivers, the focus of this study, spend nearly half.

Research Design and Methods
To better understand this disparity, we conducted 11 qualitative interviews with 14 Latino caregivers of persons living with dementia located in either California or Texas. Interview transcripts were thematically coded, guided by a material–psychosocial–behavioral conceptual model of financial strain.

Results
We identified 3 themes: daily needs and costs, psychological distress caused by financial issues, and stressful barriers to accessing family and societal support. Furthermore, interviews revealed how Latino culture may influence spending patterns and management of costs. Findings suggest that preference by Latino families to care for a family member in the home may be met with a financial disadvantage due to the high out-of-pocket costs of care.

Discussion and Implications
A better understanding of the factors contributing to high costs for Latino caregivers and how these costs affect caregivers will inform approaches at both the individual and policy levels and develop culturally relevant interventions to help Latino families to lower caregiving costs. This is especially important as the number of Latinos living with dementia is expected to increase over the next 4 decades and effective interventions are lacking.
Harish-Chandra Modules over Hopf Galois Orders
Authors: Hartwig Jonas T 
Published: February 2023

AbstractThe theory of Galois orders was introduced by Futorny and Ovsienko [9]. We introduce the notion of $\mathcal {H}$-Galois $\Lambda $-orders. These are certain noncommutative orders $F$ in a smash product of the fraction field of a noetherian integral domain $\Lambda $ by a Hopf algebra ${\mathcal {H}}$ (or, more generally, by a coideal subalgebra of a Hopf algebra). They are generalizations of Webster’s [25] principal flag orders. Examples include Cherednik algebras, as well as examples from Hopf Galois theory. We also define spherical Galois orders, which are the corresponding generalizations of principal Galois orders introduced by the author [12]. The main results are (1) for every maximal ideal $\mathfrak {m}$ of $\Lambda $ of finite codimension, there exists a simple Harish-Chandra $F$-module in the fiber of $\mathfrak {m}$; (2) for every character of $\Lambda $, we construct a canonical simple Harish-Chandra module as a subquotient of the module of local distributions; (3) if a certain stabilizer coalgebra is finite-dimensional, then the corresponding fiber of simple Harish-Chandra modules is finite; and (4) centralizers of symmetrizing idempotents are spherical Galois orders and every spherical Galois order appears that way.
Timing and distance of natal dispersal in Asian black bears
Authors: Takayama Kaede , Ohnishi Naoki , Zedrosser Andreas , Anezaki Tomoko , Tochigi Kahoko , Inagaki Akino , Naganuma Tomoko , Yamazaki Koji , Koike Shinsuke 
Published: February 2023

AbstractDispersal has important implications for population ecology and genetics of a species through redistribution of individuals. In most mammals, males leave their natal area before they reach sexual maturity, whereas females are commonly philopatric. Here, we investigate the patterns of natal dispersal in the Asian black bear (Ursus thibetanus) based on data from 550 bears (378 males, 172 females) captured or removed in Gunma and Tochigi prefectures on central Honshu Island, Japan in 2003–2018. We used genetic data and parentage analysis to investigate sex-biased differences in the distance of natal dispersal. We further investigated the age of dispersal using spatial autocorrelation analysis, that is, the change in the correlation between genetic and geographic distances in each sex and age group. Our results revealed that male dispersal distances (mean ± SE = 17.4 ± 3.5 km) were significantly farther than female distances (4.8 ± 1.7 km), and the results were not affected by years of mast failures, a prominent forage source for this population. Based on an average adult female home range radius of 1.8 km, 96% of the males and 50% of the females dispersed. In the spatial autocorrelation analysis, the changes in the relationship between genetic and geographic distances were more pronounced in males compared to females. Males seem to mostly disperse at age 3 regardless of mast productivity, and they gradually disperse far from their home range, but young and inexperienced males may return to their natal home range in years with poor food conditions. The results suggest that factors driving the dispersal process seem to be population structure-based instead of forage availability-based. In females, a significant genetic relationship was observed among all individuals in the group with a minimum age of 6 years within a distance of 2 km, which resulted in the formation of matrilineal assemblages.
A randomized trial on the effectiveness and safety of 5 water-based personal lubricants
Authors: Palacios Santiago , Hood Sarah , Abakah-Phillips Temitayo , Savania Nina , Krychman Michael 
Published: February 2023

AbstractBackgroundA range of personal lubricants with different formulations and subsequent properties are available for relief of discomfort associated with vaginal dryness; however, there are limited clinical data to support the efficacy and safety of many commercially available lubricants.AimTo determine the effectiveness and safety of 5 water-based personal lubricants for the relief of intimate discomfort associated with vaginal dryness in pre- and postmenopausal women: 4 that were formulated to meet the World Health Organization (WHO) guidelines for osmolality and pH and 1 preexisting lubricant of higher osmolality and pH.MethodsAn open-label, parallel-design study was performed in women aged 18 to 65 years with mild-to-moderate vaginal dryness and dyspareunia. Participants were randomized to 1 of 5 lubricants (A-E) from 3 brands (Durex, KY, Queen V). They were instructed to use their allocated lubricants during vaginal intercourse at least once a week over a 4-week period. The Female Sexual Function Index (FSFI) measured sexual functioning after 4 weeks of use as an indicator of lubricant performance.OutcomesThe primary outcome was change from baseline in total FSFI score after 4 weeks of product use.ResultsA total of 174 women completed the study. The primary end point—a prespecified increase in FSFI ≥4 points from baseline after 4 weeks of use—was met by all 5 lubricants tested. A statistically significant improvement was observed across all 6 domains of the FSFI from baseline to 4 weeks of use with all 5 lubricants (P &lt; .0001 for lubrication and pain reduction and P &lt; .05 for all other domains). No serious adverse events occurred in the study, and the tolerance of all 5 lubricants was good/very good.Clinical ImplicationsThe efficacy and safety of the tested lubricants are not compromised when formulated to meet the WHO criterion of osmolality ≤1200 mOsm/kg. The lubricants tested in this investigation can be used not only to relieve symptomatology of vaginal dryness and dyspareunia but also to enhance overall sexual satisfaction.Strengths and LimitationsThis study provides clinical evidence for the efficacy and safety of 5 lubricants, including those formulated to meet WHO guidelines, in relieving symptoms of vaginal dryness and improving the overall sexual experience. The open-label design may have introduced bias into the study.ConclusionAll 5 lubricants, including those formulated to be compliant with guidelines on pH and osmolality, can be considered effective and well tolerated for the relief of discomfort associated with vaginal dryness.
Characteristics of men who report symptoms of delayed ejaculation: providing support for empirically derived diagnostic criteria
Authors: Rowland David L , Attinger Drew R , Morrow Abigail L , Motofei Ion , Hevesi Krisztina 
Published: February 2023

AbstractBackgroundLittle is known regarding the demographic, sexual, and relationship characteristics of men with symptoms of delayed ejaculation (DE).AimTo identify differences between men with and without DE symptomology to validate face-valid diagnostic criteria and to identify various functional correlates of DE.MethodsA total of 2679 men meeting inclusion criteria were partitioned into groups with and without DE symptomology on the basis of their self-reported “difficulty reaching ejaculation/orgasm during partnered sex.” Men were then compared on a broad array of demographic and relationship variables, as well as sexual response variables assessed during partnered sex and masturbation.OutcomesOutcomes included the identified differences between men with and without DE symptomology.ResultsMen with DE—whether having comorbid erectile dysfunction or not—differed from men without DE on 5 face-valid variables related to previously proposed diagnostic criteria for DE, including ones related to ejaculation latency (P &lt; .001); self-efficacy related to reaching ejaculation, as assessed by the percentage of episodes reaching ejaculation during partnered sex (P &lt; .001); and negative consequences of the impairment, including “bother/distress” and (lack of) “orgasmic pleasure/sexual satisfaction” (P &lt; .001). All such differences were associated with medium to large effect sizes. In addition, men showed differences on a number of functional correlates of DE, including anxiety, relationship satisfaction, frequency of partnered sex and masturbation, and level of symptomology during partnered sex vs masturbation (P &lt; .001).Clinical ImplicationsFace-valid criteria for the diagnosis of DE were statistically verified, and functional correlates of DE relevant to guiding and focusing treatment were identified.Strengths and LimitationsIn this first comprehensive analysis of its kind, we have demonstrated widespread differences on sexual and relationship variables relevant to the diagnosis of DE and to its functional correlates between men with and without DE symptomology during partnered sex. Limitations include participant recruitment through social media, which likely biased the sample; the use of estimated rather than clocked ejaculation latencies; and the fact that differences between men with acquired and lifelong DE were not investigated.ConclusionThis well-powered multinational study provides strong empirical support for several face-valid measures for the diagnosis of DE, with a number of explanatory and control covariates that may help shed light on the lived experiences of men with DE and suggest focus areas for treatment. Whether or not the DE men had comorbid erectile dysfunction had little impact on the differences with men having normal ejaculatory functioning.
The transcription factor POPEYE negatively regulates the expression of bHLH Ib genes to maintain iron homeostasis
Authors: Pu Meng Na , Liang Gang 
Published: February 2023

Abstract
Iron (Fe) is an essential trace element for plants. When suffering from Fe deficiency, plants modulate the expression of Fe deficiency-responsive genes to promote Fe uptake. POPEYE (PYE) is a key bHLH (basic helix-loop-helix) transcription factor involved in Fe homeostasis. However, the molecular mechanism of PYE regulating the Fe deficiency response remains elusive in Arabidopsis. We found that the overexpression of PYE attenuates the expression of Fe deficiency-responsive genes. PYE directly represses the transcription of bHLH Ib genes (bHLH38, bHLH39, bHLH100, and bHLH101) by associating with their promoters. Although PYE contains an ethylene response factor-associated amphiphilic repression (EAR) motif, it does not interact with the transcriptional co-repressors TOPLESS/TOPLESS-RELATED (TPL/TPRs). Sub-cellular localization analysis indicated that PYE localizes in both the cytoplasm and nucleus. PYE contains a nuclear export signal (NES) which is required for the cytoplasmic localization of PYE. Mutation of the NES amplifies the repression function of PYE, resulting in down-regulation of Fe deficiency-responsive genes. Co-expression assays indicated that three bHLH IVc members (bHLH104, bHLH105/ILR3, and bHLH115) facilitate the nuclear accumulation of PYE. Conversely, PYE indirectly represses the transcription activation ability of bHLH IVc. Additionally, PYE directly negatively regulates its own transcription. This study provides new insights into the Fe deficiency response signalling pathway and enhances the understanding of PYE functions in Arabidopsis.
The NIN-LIKE PROTEIN 7 transcription factor modulates auxin pathways to regulate root cap development in Arabidopsis
Authors: Kumar Narender , Caldwell , Chloe , Iyer-Pascuzzi Anjali S 
Published: February 2023

Abstract
The root cap is a small tissue located at the tip of the root with critical functions for root growth. Present in nearly all vascular plants, the root cap protects the root meristem, influences soil penetration, and perceives and transmits environmental signals that are critical for root branching patterns. To perform these functions, the root cap must remain relatively stable in size and must integrate endogenous developmental pathways with environmental signals, yet the mechanism is not clear. We previously showed that low pH conditions altered root cap development, and these changes are mediated by the NIN LIKE PROTEIN 7 (NLP7) transcription factor, a master regulator of nitrate signaling. Here we show that in Arabidopsis NLP7 integrates nitrate signaling with auxin pathways to regulate root cap development. We found that low nitrate conditions promote aberrant release of root cap cells. Nitrate deficiency impacts auxin pathways in the last layer of the root cap, and this is mediated in part by NLP7. Mutations in NLP7 abolish the auxin minimum in the last layer of the root cap and alter root cap expression of the auxin carriers PIN-LIKES 3 (PILS3) and PIN-FORMED 7 (PIN7) as well as transcription factors that regulate PIN expression. Together, our data reveal NLP7 as a link between endogenous auxin pathways and nitrate signaling in the root cap.
An Exploratory Thematic Analysis of Factors That Influence Sleep in Music Therapy Undergraduate Students
Authors: Muralidharan Ananya , Silverman Michael J 
Published: February 2023

Abstract
Sleep has an essential role in the health, wellbeing, and ability to learn of undergraduate college and university students (UCUS). Due to high credit loads, the music curriculum, and music therapy competencies, music therapy students may experience difficulties obtaining adequate sleep. Therefore, the purpose of this study was to understand factors that enable or inhibit sleep in undergraduate music therapy students. We conducted semi-structured interviews with 14 junior and senior level music therapy UCUS at public institutions in the United States. We used an inductive approach to thematic analysis to analyze data and incorporated member checking and trustworthiness to clarify and verify the results. We identified three themes (supported by seven subthemes) as factors that impacted the sleep of undergraduate music therapy students: (a) academic schedule and expected time commitment; (b) intrinsic motivation to become better student music therapists; and (c) wellness-based education and personal factors. The results of this study provide insight into the various factors that might adversely affect sleep in undergraduate music therapy students and have consequences on their health, wellbeing, and learning. Implications for students and educators, limitations of the study, and suggestions for future research are provided.
Clinical outcome of biomarker-guided therapies in adult patients with tumors of the nervous system
Authors: Renovanz Mirjam , Kurz Sylvia C , Rieger Johannes , Walter Bianca , Becker Hannes , Hille Hanni , Bombach Paula , Rieger David , Grosse Lucia , Häusser Lara , Skardelly Marco , Merk Daniel J , Paulsen Frank , Hoffmann Elgin , Gani Cihan , Neumann Manuela , Beschorner Rudi , Rieß Olaf , Roggia Cristiana , Schroeder Christopher , Ossowski Stephan , Armeanu-Ebinger Sorin , Gschwind Axel , Biskup Saskia , Schulze Martin , Fend Falko , Singer Stephan , Zender Lars , Lengerke Claudia , Brucker Sara Yvonne , Engler Tobias , Forschner Andrea , Stenzl Arnulf , Kohlbacher Oliver , Nahnsen Sven , Gabernet Gisela , Fillinger Sven , Bender Benjamin , Ernemann Ulrike , Öner Öznur , Beha Janina , Malek Holly Sundberg , Möller Yvonne , Ruhm Kristina , Tatagiba Marcos , Schittenhelm Jens , Bitzer Michael , Malek Nisar , Zips Daniel , Tabatabai Ghazaleh 
Published: February 2023

AbstractBackgroundThe clinical utility of molecular profiling and targeted therapies for neuro-oncology patients outside of clinical trials is not established. We aimed at investigating feasibility and clinical utility of molecular profiling and targeted therapy in adult patients with advanced tumors in the nervous system within a prospective observational study.Methodsmolecular tumor board (MTB)@ZPM (NCT03503149) is a prospective observational precision medicine study for patients with advanced tumors. After inclusion of patients, we performed comprehensive molecular profiling, formulated ranked biomarker-guided therapy recommendations based on consensus by the MTB, and collected prospective clinical outcome data.ResultsHere, we present initial data of 661 adult patients with tumors of the nervous system enrolled by December 31, 2021. Of these, 408 patients were presented at the MTB. Molecular-instructed therapy recommendations could be made in 380/408 (93.1%) cases and were prioritized by evidence levels. Therapies were initiated in 86/380 (22.6%) cases until data cutoff. We observed a progression-free survival ratio &gt;1.3 in 31.3% of patients.ConclusionsOur study supports the clinical utility of biomarker-guided therapies for neuro-oncology patients and indicates clinical benefit in a subset of patients. Our data might inform future clinical trials, translational studies, and even clinical care.
A Functional Polymorphism in IL-1B Is Associated With Immune Reconstitution Inflammatory Syndrome of Chronic Disseminated Candidiasis
Authors: Rammaert Blandine , Bochud Pierre-Yves , Brunel Anne-Sophie , Wojtowicz Agnieszka , Candon Sophie , Gallego Hernanz Maria Pilar , Lortholary Olivier 
Published: February 2023

AbstractWe investigated single nucleotide polymorphisms (SNPs) possibly involved in immune reconstitution inflammatory syndrome of chronic disseminated candidiasis (IRIS-CDC) through a candidate gene approach and a prospective matched-control study. We found that an SNP located in interleukin-1B at rs1143627 was significantly associated with the risk of developing IRIS-CDC.
Single-cytosine methylation at W-boxes repels binding of WRKY transcription factors through steric hindrance
Authors: Charvin Magali , Halter Thierry , Blanc-Mathieu Romain , Barraud Pierre , Aumont-Nicaise Magali , Parcy François , Navarro Lionel 
Published: February 2023

Abstract
DNA methylation is an epigenetic mark that fine-tunes gene expression, notably by negatively or positively regulating transcription factor (TF)-DNA binding. In plants, DNA methylation has primarily been shown to inhibit TF-DNA binding. However, little is known about the underlying mechanisms. Here, we show that DNA methylation decreases the binding of several Arabidopsis (Arabidopsis thaliana) WRKY TFs to their genomic regions and their binding sites in vitro. We also provide evidence that DNA methylation at a single cytosine located in a functional core W-box motif repels DNA binding of AtWRKY40 in vitro. Using structural modelling, we further demonstrate that this cytosine interacts through van der Waals contacts with the conserved tyrosine of WRKY-DNA binding domains. Importantly, our model predicts steric hindrance when a 5-methyl group is present on this specific cytosine, thereby likely preventing tight binding of WRKY-DNA binding domains. Finally, because the WRKY motif and the residues involved in DNA contacts are conserved across Arabidopsis and rice (Oryza sativa) WRKY TFs, we propose that this methylation-dependent WRKY-DNA binding inhibitory mechanism could be widespread across plant species.