bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome sequencing reveals jasmonate playing a key role in ALA-induced osmotic stress tolerance in strawberry
Authors: Zhong Yan , Wei Xin , Zhang Jianting , Wang Liangju 
Published: January 2025

Abstract
Background
Strawberry (Fragaria × annanasa Duch.) is an important economic fruit worldwide, whose growth and development are often hindered by water deficiency. 5-Aminolevulinic acid (ALA), a natural plant growth regulator, has been suggested to mitigate the osmotic damages by promoting root water absorption, osmotic adjustment, photosynthetic capacity, and antioxidant improvement. However, the regulatory mechanism remains unclear.

Results
In the current study, the underlying mechanism by determination of various physiological indices, as well as transcriptome sequencing and the weighted gene correlation network analysis (WGCNA) of 10 mg L− 1 ALA treated strawberry leaves and roots stressed by 20% polyethylene glycol 6000 (PEG) treatment. The findings indicated that ALA enhanced osmotic stress tolerance reflected by enhancing relative water content (RWC), root development, gas exchange parameters and antioxidant enzyme activities, and decreasing the leaf H2O2 and malondialdehyde (MDA) content. Transcriptome analysis showed that the differentially expressed genes (DEGs) stimulated by exogenous ALA were mostly associated with the secondary biosynthesis and hormones signaling pathways, especially jasmonates (JAs). The JA derivative (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile) was found to be elevated in the strawberry leaves and roots treated with ALA under PEG stress. Additionally, exogenous methyl jasmonate (MeJA) alleviated osmotic stress damages similarly to ALA, while its synthesis inhibitor diethyldithiocarbamate (DIECA) led to adverse effects on strawberries, which can be relieved by further additional application of ALA.

Conclusions
Theses findings suggest that JAs can act as the necessary signaling molecules involved in ALA-improved osmotic stress tolerance networks. This provides a new insight for further study on how ALA can help plants cope with water stress.
Posttraumatic stress disorder in disaster-exposed youth: examining diagnostic concordance and model fit using ICD-11 and DSM-5 criteria
Authors: Danzi BreAnne A. , Knowles Ellen A. , Bock Rachel C. 
Published: January 2025

Abstract
Background
Competing definitions of posttraumatic stress disorder (PTSD) have been proposed by ICD-11 and DSM-5; it is unclear which diagnostic model works best for children and adolescents. Although other studies have predicted the impact of these models by approximating the criteria using older measures, this study advances the research by comparing measures designed to assess ICD-11 and DSM-5 criteria in hurricane-exposed youth. This study evaluates ICD-11 and DSM-5 (both the standard and preschool-age) diagnostic models by identifying diagnostic rates, evaluating diagnostic concordance, investigating the predictive value of constructs associated with PTSD (demographics, disaster threat and exposure, functional impairment), and examining model fit.

Method
The sample was exposed to Hurricane Ian (2022), a deadly Category 5 hurricane. Parents reported on disaster exposure and their child’s PTSD symptoms (n = 152; ages 7–17) using the International Trauma Questionnaire for Children and Adolescents Caregiver Version (ITQ-CG) for ICD-11 criteria and UCLA PTSD Reaction Index for DSM-5, Parent/Caregiver Report Version (RI-5) for DSM-5 criteria.

Results
ICD-11 PTSD symptom criteria rates were 24% and dropped to 20% when the impairment criterion was added. PTSD symptom criteria rates were 11% (10% with impairment) for DSM-5 and 13% (12% with impairment) for DSM-5 Preschool. ICD-11 rates were higher than DSM-5 and DSM-5 Preschool rates. There was no difference between DSM-5 and DSM-5 Preschool rates of PTSD. There was moderate to substantial concordance between ICD-11 and the DSM-5 models. All diagnostic models were associated with exposure and impairment, but only ICD-11 was associated with threat. ICD-11 was the only one to evidence acceptable model fit.

Conclusions
Using the ITQ-CG and RI-5 to assess PTSD in youth, results showed higher PTSD diagnostic rates for ICD-11 than DSM-5; this contradicts prior findings (based off approximated ICD-11 criteria) and seems largely due to differences in symptom thresholds used by the two measures. The ITQ-CG exhibited excellent model fit and was associated with several constructs important to PTSD.
Testing the effect of ecolabels on the environmental impact of food purchases in worksite cafeterias: a randomised controlled trial
Authors: Luick Madison , Stewart Cristina , Clark Michael , Bateman Paul , Biggs Elizabeth , Cook Brian , Little Melissa , Wren Gina M. , Jebb Susan A. , Pechey Rachel 
Published: January 2025

Abstract
Background
Reducing the environmental impact of foods consumed is important for meeting climate goals. We aimed to conduct a randomised controlled trial to test whether ecolabels reduce the environmental impact of food selected in worksite cafeterias, alone or in combination with increased availability of more sustainable meal options.

Methods
Worksite cafeterias (n = 96) were randomised to one of three study groups, with 54 included for final analysis. One group was intended to increase the availability of meat-free options, but no change was implemented. Therefore, this group was treated as part of the control, creating two groups: (1) control (no ecolabels) (n = 35), and (2) ecolabels (n = 19). Regression analysis assessed the primary outcome of total environmental impact of hot meals sold over a 6-week period. Secondary outcome analyses explored the individual environmental indicators that composed the total environmental impact score (i.e., greenhouse gas emissions, biodiversity loss, eutrophication, and water scarcity). The mean weekly environmental impact scores of hot meal options over the full 12-week trial period were assessed using hierarchical mixed effects models.

Results
There was no significant effect of the intervention on the environmental impact scores of meals sold (mean difference between control and intervention sites: -1.4%, 95%CI: -33.6%, + 30.8%). There was no evidence of an effect in mean weekly environmental impact score (-5.4%, 95%CI: -12.6%, + 2.5%), nor in any of the four individual environmental indicators (greenhouse gas emissions: -3.6%, 95%CI: -30.7%, 34.3%; biodiversity loss: 2.0%, 95%CI: -25.8%, 40.2%; eutrophication: -2.4%, 95%CI: -29.3%, 34.7%; water scarcity: -0.4%, 95%CI: -28.7%, 39.1%).

Conclusions
Ecolabels may not be an effective tool to shift consumer behaviour in worksite cafeterias towards meals with lower environmental impact.

Trial registration
The study was pre-registered prospectively on ISRCTN (https://www.isrctn.com/ISRCTN10268258; 06/01/2022).
Suppression of mycotoxins production and efficient chelation of heavy metals using natural melanin originated from Aspergillus flavus and Aspergillus carbonarius
Authors: El-Gazzar Nashwa , Abdo Esraa , Rabie Gamal , El-Sayed Manal Tawfeek 
Published: January 2025

Abstract
Background
This study employed melanin synthesized by Aspergillus flavus and Aspergillus carbonarius to inhibit the production of mycotoxins and bioremediation of heavy metals (HMs).

Methods
First, twenty fungal isolates were obtained from soil samples, and were evaluated to produce melanin. The melanin of the most potent producers has undergone several confirmatory experiments, including, Dihydroxyphenylalanine (DOPA)-inhibitor-kojic acid pathway detection, High-performance liquid chromatography (HPLC), Fourier-transform infrared (FTIR) and Nuclear magnetic resonance (NMR). Additionally, the melanin production culture conditions were optimized. The antioxidant activity of melanin was detected with 1,1-Diphenyl-2-picrylhydrazyl (DPPH). HPLC was used to measure the mycotoxins produced in culture media supplemented with melanin. Molecular docking study investigated molecular interactions between melanin and mycotoxins through in silico approaches. FTIR and Energy-dispersive X-ray spectroscopy (EDX) were utilized to determine the percentage of melanin-chelated HMs, and an atomic absorption spectrophotometer (AAS) was used to detect HMs removal efficiency.

Results
The melanin-enriched medium (0.3% and 0.4%) exhibited complete inhibition of aflatoxin B1 (AF-B1) by A. flavus and ochratoxin A (OTA) by A. carbonarius, respectively. Furthermore, melanin showed effective HM removal efficiency, increasing with melanin concentration. The removal efficiency of Cd+2 and Cr+6 by 1 mg/mL melanin was 49% and 63%, respectively. When the concentration of melanin was increased to 15 mg/mL, the removal efficiency of Cd+2 and Cr+2 increased to 60% and 77%, respectively.

Conclusion
The study exhibited a natural approach for melanin production, using melanin as a heavy metal-chelating agent and capability to inhibit the production of aflatoxin B1 and ochratoxin A. Further, the study provides significant evidence regarding the bioremediation pipeline, for melanin production through biotechnological processes by filamentous fungi.
Clinical outcomes of a digital musculoskeletal women’s pelvic health program: an observational, longitudinal study with comparison group
Authors: Hong Mindy , Kirk Rachel Foster , Toprani Bijal , Sweet Cynthia Castro , Pan Clare , Krauss Jeffrey , Grisales Tamara 
Published: January 2025

Abstract
Background
Chronic pelvic pain is a common yet undertreated condition that significantly impacts quality of life for women worldwide. Digital exercise therapy designed to target pelvic pain can improve symptomology while reducing time and cost-related barriers to in-person clinical care.

Methods
This longitudinal, observational study of a digital women’s pelvic health program examined pelvic pain, anxiety, and depression at 4 and 12 weeks in female adults experiencing chronic pelvic pain. Intervention participants received a digital pelvic health program including personalized exercise therapy sessions, health education articles, and health coaching. A comparison group of nonparticipants received a series of education articles related to pelvic health. Data were collected at baseline, 4 and 12 weeks. Unadjusted and adjusted linear mixed effects models were conducted to model changes in clinical outcomes over time.

Results
A total of 797 participants (intervention: 495, nonparticipants: 302) were included in the sample. Baseline mean (SD) age was 41.5 (11.7) years and mean pain was 45.7 (18.5) out of 100. Compared to baseline, the intervention group showed significantly more pain improvement at 4 and 12 weeks versus nonparticipants after adjusting for baseline factors. The intervention group’s pain scores decreased by 44.5% at 4 weeks and 53.6% at 12 weeks. The intervention group’s adjusted pain scores decreased from 42.0 (95% CI: [39.4, 44.7]) at baseline to 23.3 (95% CI: [20.5, 26.2]) at 4 weeks to 19.5 (95% CI: [16.7, 22.4]) at 12 weeks. In contrast, nonparticipants’ pain scores decreased by 21.6% at 4 weeks and 32.7% at 12 weeks. Nonparticipants’ adjusted pain scores decreased from 42.1 (95% CI: [38.4, 45.9]) at baseline to 33.0 (95% CI: [29.2, 36.8]) at 4 weeks to 28.3 (95% CI: [24.5, 32.2]) at 12 weeks. After adjustments, the probability of the intervention group screening for moderate or severe depression was significantly lower by 11.0% at 12 weeks versus nonparticipants. There were no significant differences in anxiety outcomes between groups at baseline, week 4, or week 12.

Conclusions
A digital women’s pelvic health program may help reduce short-term pelvic pain and depression symptoms.

Trial registration
The WIRB-Copernicus Group Institutional Review Board (registration number IRB20234932) approved this study on November 6, 2023.
Addressing evidence needs during health crises in the province of Quebec (Canada): a proposed action plan for rapid evidence synthesis
Authors: Hong Quan Nha , McSween-Cadieux Esther , Guillette Maxime , Manceau Luiza Maria , Li Jingjing , Granikov Vera , Pomey Marie-Pascale , Gagnon Marie-Pierre , Ziam Saliha , Dagenais Christian , Dagenais Pierre , Lesage Alain , Poder Thomas G. , Drapeau Martin , Ridde Valéry , Lane Julie 
Published: January 2025

Abstract
Background
The COVID-19 pandemic necessitated the rapid availability of evidence to respond in a timely manner to the needs of practice settings and decision-makers in health and social services. Now that the pandemic is over, it is time to put in place actions to improve the capacity of systems to meet knowledge needs in a situation of crisis. The main objective of this project was thus to develop an action plan for the rapid syntheses of evidence in times of health crisis in Quebec (Canada).

Methods
We conducted a three-phase collaborative research project. First, we carried out a survey with producers and users of rapid evidence syntheses (n = 40) and a group interview with three patient partners to prioritize courses of action. In parallel, we performed a systematic mapping of the literature to identify rapid evidence synthesis initiatives developed during the pandemic. The results of these two phases were used in a third phase, in which we organized a deliberative workshop with 26 producers and users of rapid evidence syntheses to identifying strategies to operationalize priorities. The data collected at each phase were compared to identify common courses of action and integrated to develop an action plan.

Results
A total of 14 specific actions structured into four main axes were identified over the three phases. In axis 1, actions on raising awareness of the importance of evidence-informed decision-making among stakeholders in the health and social services network are presented. Axis 2 includes actions to promote optimal collaboration of key stakeholders in the production of rapid evidence synthesis to support decision-making. Actions advocating the use of a variety of rapid evidence synthesis methodologies known to be effective in supporting decision-making are presented in axis 3. Finally, axis 4 is about actions on the use of effective knowledge translation strategies to promote the use of rapid evidence synthesis products to support decision-making.

Conclusions
This project led to the development of a collective action plan aimed at preparing the Quebec ecosystem and other similar jurisdictions to meet knowledge needs more effectively in times of health emergency. The implementation of this plan and its evaluation will enable us to continue to fine-tune it.
The association between lipid-related obesity indicators and severe headache or migraine: a nationwide cross sectional study from NHANES 1999 to 2004
Authors: Sun Xu , Song Jimei , Yan Rixun , Diao Jianwei , Liu Yibo , Zhu Zhangzhi , Lu Weichi 
Published: January 2025

Abstract
Background
The connection between lipid-related obesity indices and severe headache or migraine in young and middle-aged people aged 20–60 remains ambiguous, and there are gaps in the discriminative ability of different indicators for severe headaches or migraines. Consequently, we set out to look into this association utilizing National Health and Nutrition Examination Survey (NHANES) data from 1999 to 2004.

Methods
After the values of waist-to-height ratio (WHtR), body-mass index (BMI), body roundness index (BRI), visceral adiposity index (VAI), lipid accumulation product (LAP), triglyceride glucose index (TyG), cardiac metabolism index (CMI), waist triglyceride Index (WTI), conicity index (CI) and weight-adjusted waist index (WWI) were estimated, with minimal sufficient adjustment for confounders determined by directed acyclic graph (DAG), weighted univariable and multivariable logistic regression analyses were carried out to ascertain the relationship between them and migraine. Stratified analysis and cross-effect analysis were implemented to examine the variability of intergroup correlations. Restricted cubic splines (RCS) and receiver operating characteristic (ROC) were then employed to examine nonliner relationships and its discriminatory ability for severe headache or migraine, respectively.

Results
3354 United States adults were involved in our study, of whom 839 (25.01%) had severe headache or migraine. After adjusting for relevant covariables, WHtR, BRI, BMI, LAP, WTI and VAI were all associated with migraine and WHtR (OR = 6.38, 95% CI: 2.25,18.09, P < 0.01) showed the best predictive ability. Additionally, WHtR, BMI, and BRI demonstrated linear dose-response relationships with the prevalence of migraine (all Poverall < 0.05, Pnon−linearity > 0.05).

Conclusions
Among those ten lipid-related obesity indicators evaluated in the study, WHtR, BMI and BRI demonstrated linear positive dose-response relationships with the prevalence of migraine in young and middle-aged individuals within the United States and WHtR showed the best predictive ability. Our study can provide important insight into epidemiological research and comprehensive management of obese patients with migraine.
Strengthening global health preparedness amid Mpox spread in Africa
Authors: Abdulrahim Abdulrakib , Gulumbe Bashar Haruna , Idris Ibrahim , Abubakar Tirmizhi Munkaila , Sobur Kazi Abdus , Lawal Abdulshakur Isah , Shehu Aminu 
Published: January 2025

AbstractThe resurgence of Mpox, formerly known as monkeypox, has emerged as a critical public health concern, driven by the more transmissible and virulent clade Ib strain. The outbreak, which has escalated across Central and West Africa, poses significant threats to global health security, primarily due to cross-border transmission and the inadequacies of current public health responses. This commentary examined the epidemiological trends, public health challenges, and gaps in containment efforts, offering insights and recommendations for a more effective global response. Drawing on data from the World Health Organization, this commentary reviews the epidemiological patterns of Mpox cases reported across 19 African nations from January to November 2024. In addition, it evaluates public health responses, including the challenges of vaccine distribution, diagnostic capacity, and healthcare system limitations, and discusses the broader implications for global preparedness. Despite emergency declarations and the mobilization of vaccine resources, efforts remain constrained by logistical barriers, limited vaccine availability, and underdeveloped diagnostic infrastructures. Furthermore, vaccine hesitancy, driven by misinformation and social stigma, continues to undermine containment measures. The current Mpox outbreak highlights substantial gaps in global health preparedness and inequities in access to essential health interventions. This commentary emphasizes the need for globally coordinated and equitable interventions to close these gaps, strengthen surveillance, improve vaccine distribution, and enhance cross-border public health measures. Without urgent action, the risk of further escalation and the global spread of Mpox remains a critical concern.
Effects of continuous positive airway pressure treatment on arterial stiffness and inflammatory factors in patients with coronary heart disease complicated with obstructive sleep apnea
Authors: Wang Liang , Wang Yuanqi , Jiao Tiantian , Xu Linghao , Ji Endong , Tapu Sakibur Rahman , Liu Yehong , Li Jiming 
Published: January 2025

Abstract
Background
Continuous Positive Airway Pressure (CPAP) treatment brings more benefits than risks to most coronary heart disease (CHD) patients with obstructive sleep apnea (OSA). However, the pathophysiological mechanism by which CPAP treatment improves the prognosis of patients with CHD and OSA remains unclear. The purpose of this study was to clarify whether CPAP can improve arterial stiffness and inflammatory factor levels in CHD patients with OSA, and to further improve prognosis.

Method
59 patients with coronary heart disease complicated by moderate to severe sleep apnea were divided into a CPAP treatment group (CPAP + coronary heart disease standard treatment) and a control group (only coronary heart disease standard treatment). Peripheral blood test reports were collected and pulse wave velocity (PWV) measurements were performed for each patient at the beginning, 3 months, and 6 months of treatment.

Results
After 6 months of treatment, the CPAP group showed more significant improvement in the levels of inflammatory factors such as white blood cell (WBC), neutrophil (N), C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT), and PWV than the control group.

Conclusion
After active treatment with CPAP, arterial stiffness and inflammatory cytokine levels in patients with coronary heart disease and OSA improved. This association should be given more attention in clinical practice, and sleep apnea should be actively treated.
PRMT5 inhibition has a potent anti-tumor activity against adenoid cystic carcinoma of salivary glands
Authors: Mishra Vasudha , Singh Alka , Korzinkin Michael , Cheng Xiangying , Wing Claudia , Sarkisova Viktoria , Koppayi Ashwin L. , Pogorelskaya Alexandra , Glushchenko Oksana , Sundaresan Manu , Thodima Venkat , Carter Jack , Ito Koichi , Scherle Peggy , Trzcinska Anna , Ozerov Ivan , Vokes Everett E. , Cole Grayson , Pun Frank W. , Shen Le , Miao Yuxuan , Pearson Alexander T. , Lingen Mark W. , Ruggeri Bruce , Rosenberg Ari J. , Zhavoronkov Alex , Agrawal Nishant , Izumchenko Evgeny 
Published: January 2025

Abstract
Background
Adenoid cystic carcinoma (ACC) is a rare glandular malignancy, commonly originating in salivary glands of the head and neck. Given its protracted growth, ACC is usually diagnosed in advanced stage. Treatment of ACC is limited to surgery and/or adjuvant radiotherapy, which often fails to prevent disease recurrence, and no FDA-approved targeted therapies are currently available. As such, identification of new therapeutic targets specific to ACC is crucial for improved patients’ outcomes.

Methods
After thoroughly evaluating the gene expression and signaling patterns characterizing ACC, we applied PandaOmics (an AI-driven software platform for novel therapeutic target discovery) on the unique transcriptomic dataset of 87 primary ACCs. Identifying protein arginine methyl transferase 5 (PRMT5) as a putative candidate with the top-scored druggability, we next determined the applicability of PRMT5 inhibitors (PRT543 and PRT811) using ACC cell lines, organoids, and patient derived xenograft (PDX) models. Molecular changes associated with response to PRMT5 inhibition and anti-proliferative effect of the combination therapy with lenvatinib was then analyzed.

Results
Using a comprehensive AI-powered engine for target identification, PRMT5 was predicted among potential therapeutic target candidates for ACC. Here we show that monotherapy with selective PRMT5 inhibitors induced a potent anti-tumor activity across several cellular and animal models of ACC, which was paralleled by downregulation of genes associated with ACC tumorigenesis, including MYB and MYC (the recognized drivers of ACC progression). Furthermore, as a subset of genes targeted by lenvatinib is upregulated in ACC, we demonstrate that addition of lenvatinib enhanced the growth inhibitory effect of PRMT5 blockade in vitro, suggesting a potential clinical benefit for patients expressing lenvatinib favorable molecular profile.

Conclusion
Taken together, our study underscores the role of PRMT5 in ACC oncogenesis and provides a strong rationale for the clinical development of PRMT5 inhibitors as a targeted monotherapy or combination therapy for treatment of patients with this rare disease, based on the analysis of their underlying molecular profile.