bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beta traveling waves in monkey frontal and parietal areas encode recent reward history
Authors: Zabeh Erfan , Foley Nicholas C. , Jacobs Joshua , Gottlieb Jacqueline P. 
Published: September 2023

AbstractBrain function depends on neural communication, but the mechanisms of this communication are not well understood. Recent studies suggest that one form of neural communication is through traveling waves (TWs)—patterns of neural oscillations that propagate within and between brain areas. We show that TWs are robust in microarray recordings in frontal and parietal cortex and encode recent reward history. Two adult male monkeys made saccades to obtain probabilistic rewards and were sensitive to the (statistically irrelevant) reward on the previous trial. TWs in frontal and parietal areas were stronger in trials that followed a prior reward versus a lack of reward and, in the frontal lobe, correlated with the monkeys’ behavioral sensitivity to the prior reward. The findings suggest that neural communication mediated by TWs within the frontal and parietal lobes contribute to maintaining information about recent reward history and mediating the impact of this history on the monkeys’ expectations.
Systematic transcriptional analysis of human cell lines for gene expression landscape and tumor representation
Authors: Jin Han , Zhang Cheng , Zwahlen Martin , von Feilitzen Kalle , Karlsson Max , Shi Mengnan , Yuan Meng , Song Xiya , Li Xiangyu , Yang Hong , Turkez Hasan , Fagerberg Linn , Uhlén Mathias , Mardinoglu Adil 
Published: September 2023

AbstractCell lines are valuable resources as model for human biology and translational medicine. It is thus important to explore the concordance between the expression in various cell lines vis-à-vis human native and disease tissues. In this study, we investigate the expression of all human protein-coding genes in more than 1,000 human cell lines representing 27 cancer types by a genome-wide transcriptomics analysis. The cell line gene expression is compared with the corresponding profiles in various tissues, organs, single-cell types and cancers. Here, we present the expression for each cell line and give guidance for the most appropriate cell line for a given experimental study. In addition, we explore the cancer-related pathway and cytokine activity of the cell lines to aid human biology studies and drug development projects. All data are presented in an open access cell line section of the Human Protein Atlas to facilitate the exploration of all human protein-coding genes across these cell lines.
A recyclable polyester library from reversible alternating copolymerization of aldehyde and cyclic anhydride
Authors: Zhang Xun , Guo Wenqi , Zhang Chengjian , Zhang Xinghong 
Published: September 2023

AbstractOur society is pursuing chemically recyclable polymers to accelerate the green revolution in plastics. Here, we develop a recyclable polyester library from the alternating copolymerization of aldehyde and cyclic anhydride. Although these two monomer sets have little or no thermodynamic driving force for homopolymerization, their copolymerization demonstrates the unexpected alternating characteristics. In addition to readily available monomers, the method is performed under mild conditions, uses common Lewis/Brønsted acids as catalysts, achieves the facile tuning of polyester structure using two distinct monomer sets, and yields 60 polyesters. Interestingly, the copolymerization exhibits the chemical reversibility attributed to its relatively low enthalpy, which makes the resulting polyesters perform closed-loop recycling to monomers at high temperatures. This study provides a modular, efficient, and facile synthesis of recyclable polyesters using sustainable monomers.
Aryl hydrocarbon receptor utilises cellular zinc signals to maintain the gut epithelial barrier
Authors: Hu Xiuchuan , Xiao Wenfeng , Lei Yuxian , Green Adam , Lee Xinyi , Maradana Muralidhara Rao , Gao Yajing , Xie Xueru , Wang Rui , Chennell George , Basson M. Albert , Kille Pete , Maret Wolfgang , Bewick Gavin A. , Zhou Yufeng , Hogstrand Christer 
Published: September 2023

AbstractZinc and plant-derived ligands of the aryl hydrocarbon receptor (AHR) are dietary components affecting intestinal epithelial barrier function. Here, we explore whether zinc and the AHR pathway are linked. We show that dietary supplementation with an AHR pre-ligand offers protection against inflammatory bowel disease in a mouse model while protection fails in mice lacking AHR in the intestinal epithelium. AHR agonist treatment is also ineffective in mice fed zinc depleted diet. In human ileum organoids and Caco-2 cells, AHR activation increases total cellular zinc and cytosolic free Zn2+ concentrations through transcription of genes for zinc importers. Tight junction proteins are upregulated through zinc inhibition of nuclear factor kappa-light-chain-enhancer and calpain activity. Our data show that AHR activation by plant-derived dietary ligands improves gut barrier function at least partly via zinc-dependent cellular pathways, suggesting that combined dietary supplementation with AHR ligands and zinc might be effective in preventing inflammatory gut disorders.
Demonstrating paths for unlocking the value of cloud genomics through cross cohort analysis
Authors: Deflaux Nicole , Selvaraj Margaret Sunitha , Condon Henry Robert , Mayo Kelsey , Haidermota Sara , Basford Melissa A. , Lunt Chris , Philippakis Anthony A. , Roden Dan M. , Denny Joshua C. , Musick Anjene , Collins Rory , Allen Naomi , Effingham Mark , Glazer David , Natarajan Pradeep , Bick Alexander G. 
Published: September 2023

AbstractRecently, large scale genomic projects such asAll of Usand the UK Biobank have introduced a new research paradigm where data are stored centrally in cloud-based Trusted Research Environments (TREs). To characterize the advantages and drawbacks of different TRE attributes in facilitating cross-cohort analysis, we conduct a Genome-Wide Association Study of standard lipid measures using two approaches: meta-analysis and pooled analysis. Comparison of full summary data from both approaches with an external study shows strong correlation of known loci with lipid levels (R2 ~ 83–97%). Importantly, 90 variants meet the significance threshold only in the meta-analysis and 64 variants are significant only in pooled analysis, with approximately 20% of variants in each of those groups being most prevalent in non-European, non-Asian ancestry individuals. These findings have important implications, as technical and policy choices lead to cross-cohort analyses generating similar, but not identical results, particularly for non-European ancestral populations.
The association between parental internalizing disorders and child school performance
Authors: Nordmo Magnus , Kleppestø Thomas , Sunde Hans Fredrik , Flatø Martin , Demange Perline , Torvik Fartein Ask 
Published: September 2023

AbstractParents play a crucial role in children’s lives. Despite high prevalences of anxiety and depression, we do not know how these disorders among parents associate with child school performance in Norway. We use regression models to estimate associations between parental mental disorders and child school performance, while adjusting for some social and genetic confounders. Parental anxiety and depression were assessed from administrative registers of government funded health service consultations for all individuals in Norway with children born between 1992 and 2002. School performance was assessed as standardized grade point average at the end of compulsory education when children are 16 years old. Associations were also considered in samples of adoptees and among differentially affected siblings. We find that 18.8% of children have a parent with an anxiety or depression diagnosis from primary care during the last three years of compulsory education (yearly prevalence: 11.5%). There is a negative association between these parental mental disorders and child school outcomes (z = 0.43). This association was weakened, but statistically significant among differentially exposed siblings (z = 0.04), while disappearing in adoptee children. Many children experience that their parents have anxiety or depression and receive a diagnosis from primary care. On average, these children have lower school performance. The association is attenuated when comparing differentially exposed siblings and disappears in adoptee children. These results have a poor fit with the hypothesis that parental internalizing is an influential causal factor in determining children’s educational success.
A next-generation GMMA-based vaccine candidate to fight shigellosis
Authors: Rossi Omar , Citiulo Francesco , Giannelli Carlo , Cappelletti Emilia , Gasperini Gianmarco , Mancini Francesca , Acquaviva Alessandra , Raso Maria Michelina , Sollai Luigi , Alfini Renzo , Aruta Maria Grazia , Vitali Claudia Giorgina , Pizza Mariagrazia , Necchi Francesca , Rappuoli Rino , Martin Laura B. , Berlanda Scorza Francesco , Colucci Anna Maria , Micoli Francesca 
Published: September 2023

AbstractShigellosis is a leading cause of diarrheal disease in low-middle-income countries (LMICs). Effective vaccines will help to reduce the disease burden, exacerbated by increasing antibiotic resistance, in the most susceptible population represented by young children. A challenge for a broadly protective vaccine against shigellosis is to cover the most epidemiologically relevant serotypes among >50 Shigella serotypes circulating worldwide. The GMMA platform has been proposed as an innovative delivery system for Shigella O-antigens, and we have developed a 4-component vaccine against S. sonnei, S. flexneri 1b, 2a and 3a identified among the most prevalent Shigella serotypes in LMICs. Driven by the immunogenicity results obtained in clinic with a first-generation mono-component vaccine, a new S. sonnei GMMA construct was generated and combined with three S. flexneri GMMA in a 4-component Alhydrogel formulation (altSonflex1-2-3). This formulation was highly immunogenic, with no evidence of negative antigenic interference in mice and rabbits. The vaccine induced bactericidal antibodies also against heterologous Shigella strains carrying O-antigens different from those included in the vaccine. The Monocyte Activation Test used to evaluate the potential reactogenicity of the vaccine formulation revealed no differences compared to the S. sonnei mono-component vaccine, shown to be safe in several clinical trials in adults. A GLP toxicology study in rabbits confirmed that the vaccine was well tolerated. The preclinical study results support the clinical evaluation of altSonflex1-2-3 in healthy populations, and a phase 1–2 clinical trial is currently ongoing.
Ultra-high capacity, multifunctional nanoscale sorbents for PFOA and PFOS treatment
Authors: Lee Junseok , Kim Changwoo , Liu Chen , Wong Michael S. , Cápiro Natalie L. , Pennell Kurt D. , Fortner John D. 
Published: September 2023

AbstractHere, we describe surface functionalized, superparamagnetic iron oxide nanocrystals (IONCs) for ultra-high PFAS sorption and precise, low energy (magnetic) separation, considering perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS). As a function of surface coating, sorption capacities described are considerably higher than previous studies using activated carbon, polymers, and unmodified metal/metal oxides, among others. In particular, positively charged polyethyleneimine (PEI) coated IONCs demonstrate extreme sorption capacities for both PFOA and PFOS due to electrostatic and hydrophobic interactions, along with high polymer grafting densities, while remaining stable in water, thus maintaining available surface area. Further, through a newly developed method using a quart crystal microbalance with dissipation (QCM-D), we present real-time, interfacial observations (e.g., sorption kinetics). Through this method, we explore underpinning mechanism(s) for differential PFAS (PFOA vs PFOS) sorption behavior(s), demonstrating that PFAS functional head group strongly influence molecular orientation on/at the sorbent interface. The effects of water chemistry, including pH, ionic composition of water, and natural organic matter on sorption behavior are also evaluated and along with material (treatment) demonstration via bench-scale column studies.
Selected physical and chemical cleanings remove biofilm in seawater membrane distillation without causing pore wetting
Authors: Amin Najat A. , Elcik Harun , Alpatova Alla , Gonzalez-Gil Graciela , Blankert Bastiaan , Farhat Nadia , Vrouwenvelder Johannes S. , Ghaffour Noreddine 
Published: September 2023

AbstractMembrane distillation (MD) is an emerging process with a proven ability to recover freshwater from streams with a wide range of salinities. However, MD is susceptible to biofouling. This study explores the efficiency of different cleaning strategies in biofilm removal during seawater MD. Hydraulic cleaning and chemical cleanings with 0.3%w w−1 ethylenediaminetetraacetic acid (EDTA), 0.3%w w−1 NaOCl, and 3%w w−1 citric acid were tested. The results showed that permeate flux recovery increased in the order of hydraulic cleaning <3%w w−1 citric acid <0.3%w w−1 NaOCl ≈0.3%w w−1 EDTA. Membrane cleanings substantially reduced the thickness of the residual biofilm layer and decreased its bacterial concentration and resistance to vapor pressure. The post-cleaning permeate conductivities were low suggesting that employed cleaning protocols did not cause pore wetting of hydrophobic polytetrafluoroethylene microporous (0.22 μm) membrane, and membrane rejection properties remained stable.
A large EEG database with users’ profile information for motor imagery brain-computer interface research
Authors: Dreyer Pauline , Roc Aline , Pillette Léa , Rimbert Sébastien , Lotte Fabien 
Published: September 2023

AbstractWe present and share a large database containing electroencephalographic signals from 87 human participants, collected during a single day of brain-computer interface (BCI) experiments, organized into 3 datasets (A, B, and C) that were all recorded using the same protocol: right and left hand motor imagery (MI). Each session contains 240 trials (120 per class), which represents more than 20,800 trials, or approximately 70 hours of recording time. It includes the performance of the associated BCI users, detailed information about the demographics, personality profile as well as some cognitive traits and the experimental instructions and codes (executed in the open-source platform OpenViBE). Such database could prove useful for various studies, including but not limited to: (1) studying the relationships between BCI users’ profiles and their BCI performances, (2) studying how EEG signals properties varies for different users’ profiles and MI tasks, (3) using the large number of participants to design cross-user BCI machine learning algorithms or (4) incorporating users’ profile information into the design of EEG signal classification algorithms.
Accurate GW frontier orbital energies of 134 kilo molecules
Authors: Fediai Artem , Reiser Patrick , Peña Jorge Enrique Olivares , Friederich Pascal , Wenzel Wolfgang 
Published: September 2023

AbstractHOMO and LUMO energies are critical molecular properties that typically require high accuracy computations for practical applicability. Until now, a comprehensive dataset containing sufficiently accurate HOMO and LUMO energies has been unavailable. In this study, we introduce a new dataset of HOMO/LUMO energies for QM9 compounds, calculated using the GW method. The GW method offers adequate HOMO/LUMO prediction accuracy for diverse applications, exhibiting mean unsigned errors of 100 meV in the GW100 benchmark dataset. This database may serve as a benchmark of HOMO/LUMO prediction, delta-learning, and transfer learning, particularly for larger molecules where GW is the most accurate but still numerically feasible method. We anticipate that this dataset will enable the development of more accurate machine learning models for predicting molecular properties.
Improvement of morning headache in adults with obstructive sleep apnea after positive airway pressure therapy
Authors: Seo Min Young , Lee Min Kyu , Han Mun Soo , Yoo Jun , Lee Seung Hoon 
Published: September 2023

AbstractThe aim of this study was to evaluate the association between obstructive sleep apnea and morning headache and to assess the improvement of morning headache following positive airway pressure therapy. One hundred and sixteen participants were enrolled in this study; all of them received positive airway pressure therapy for at least 3 months. We checked the differences in various sleep apnea-related parameters according to the presence of morning headache and evaluated the improvement of morning headache following positive airway pressure therapy. Among the 116 study participants, 103 were men, with a mean age and body mass index of 50.34 ± 10.23 years and 28.00 ± 4.21 kg/m2, respectively. The severity of morning headache was higher in the severe obstructive sleep apnea group than in the mild to moderate group (2.16 ± 1.70 vs. 1.50 ± 1.57, P = 0.027). However, the various polysomnographic parameters did not significantly differ according to the presence of headache. The Epworth sleepiness scale score was significantly higher in the morning headache presence group than in the absence group (10.90 ± 5.45 vs. 8.13 ± 4.27, P = 0.003). Furthermore, a notable correlation was observed between the reduction in daytime sleepiness and the improvement in morning headache following PAP treatment (r = 0.503, P < 0.001). Morning headache significantly improved following positive airway pressure therapy (prevalence: 53.4–16.4%; severity: 1.92 ± 1.67 vs. 0.86 ± 0.80, all P < 0.001), especially in the patients with morning headache before positive airway pressure therapy. Morning headache is significantly associated with daytime sleepiness and positive airway pressure therapy improves morning headache.
Combining glucose and high-sensitivity cardiac troponin in the early diagnosis of acute myocardial infarction
Authors: Yufera-Sanchez Ana , Lopez-Ayala Pedro , Nestelberger Thomas , Wildi Karin , Boeddinghaus Jasper , Koechlin Luca , Rubini Gimenez Maria , Sakiz Hüseyin , Bima Paolo , Miro Oscar , Martín-Sánchez F. Javier , Christ Michael , Keller Dagmar I. , Gualandro Danielle M. , Kawecki Damian , Rentsch Katharina , Buser Andreas , Mueller Christian ,   The APACE Investigators
Published: September 2023

AbstractGlucose is a universally available inexpensive biomarker, which is increased as part of the physiological stress response to acute myocardial infarction (AMI) and may therefore help in its early diagnosis. To test this hypothesis, glucose, high-sensitivity cardiac troponin (hs-cTn) T, and hs-cTnI were measured in consecutive patients presenting with acute chest discomfort to the emergency department (ED) and enrolled in a large international diagnostic study (NCT00470587). Two independent cardiologists centrally adjudicated the final diagnosis using all clinical data, including serial hs-cTnT measurements, cardiac imaging and clinical follow-up. The primary diagnostic endpoint was index non-ST-segment elevation MI (NSTEMI). Prognostic endpoints were all-cause death, and cardiovascular (CV) death or future AMI, all within 730-days. Among 5639 eligible patients, NSTEMI was the adjudicated final diagnosis in 1051 (18.6%) patients. Diagnostic accuracy quantified using the area under the receiver-operating characteristics curve (AUC) for the combination of glucose with hs-cTnT and glucose with hs-cTnI was very high, but not higher versus that of hs-cTn alone (glucose/hs-cTnT 0.930 [95% CI 0.922–0.937] versus hs-cTnT 0.929 [95% CI 0.922–0.937]; glucose/hs-cTnI 0.944 [95% CI 0.937–0.951] versus hs-cTnI 0.944 [95% CI 0.937–0.951]). In early-presenters, a dual-marker strategy (glucose < 7 mmol/L and hs-cTnT < 5/hs-cTnI < 4 ng/L) provided very high and comparable sensitivity to slightly lower hs-cTn concentrations (cTnT/I < 4/3 ng/L) alone, and possibly even higher efficacy. Glucose was an independent predictor of 730-days endpoints. Our results showed that a dual marker strategy of glucose and hs-cTn did not increase the diagnostic accuracy when used continuously. However, a cutoff approach combining glucose and hs-cTn may provide diagnostic utility for patients presenting ≤ 3 h after onset of symptoms, also providing important prognostic information.
Measuring picometre-level displacements using speckle patterns produced by an integrating sphere
Authors: Facchin Morgan , Bruce Graham D. , Dholakia Kishan 
Published: September 2023

AbstractAs the fields of optical microscopy, semiconductor technology and fundamental science increasingly aim for precision at or below the nanoscale, there is a burgeoning demand for sub-nanometric displacement and position sensing. We show that the speckle patterns produced by multiple reflections of light inside an integrating sphere provide an exceptionally sensitive probe of displacement. We use an integrating sphere split into two independent hemispheres, one of which is free to move in any given direction. The relative motion of the two hemispheres produces a change in the speckle pattern from which we can analytically infer the amplitude of the displacement. The method allows a noise floor of 5 pm/$$\sqrt{\hbox {Hz}}$$

Hz

($$\lambda /160,000$$

λ
/
160
,
000

) above 30 Hz in a facile implementation, which we use to measure oscillations of 17 pm amplitude ($$\lambda /50,000$$

λ
/
50
,
000

) with a signal to noise ratio of 3.
Co-administration of an effector antibody enhances the half-life and therapeutic potential of RNA-encoded nanobodies
Authors: Thran Moritz , Pönisch Marion , Danz Hillary , Horscroft Nigel , Ichtchenko Konstantin , Tzipori Saul , Shoemaker Charles B. 
Published: September 2023

AbstractThe incidence of Clostridioides difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. Here we report on the development of mRNA-LNPs encoding camelid-derived VHH-based neutralizing agents (VNAs) targeting toxins A and/or B of C. difficile. In preclinical models, intravenous administration of the mRNA-LNPs provided serum VNA levels sufficient to confer protection of mice against severe disease progression following toxin challenge. Furthermore, we employed an mRNA-LNP encoded effector antibody, a molecular tool designed to specifically bind an epitopic tag linked to the VNAs, to prolong VNA serum half-life. Co-administration of VNA-encoding mRNA-LNPs and an effector antibody, either provided as recombinant protein or encoded by mRNA-LNP, increased serum VNA half-life in mice and in gnotobiotic piglets. Prolonged serum half-life was associated with higher concentrations of serum VNA and enhanced prophylactic protection of mice in challenge models.