bluuebunny/crossref_metadata_2025_split huggingface dataset:
868 Analysis of the Surgical Edge of Adult-Type Diffuse Astrocytomas and its Correlation With Prognosis
Authors: Salazar Ramirez Zita Elizabeth , Janis Sandoval Ramirez Christian J. , Feria Romero Iris Angelica , Nettel-Rueda Barbara 
Published: March 2023

INTRODUCTION:
Glioma cells extend beyond the macroscopic margins of the tumor, making it impossible to pursue a cure based only on surgical treatment in patients with the diagnosis of Adult-Type Diffuse Glioma (ATDG). The tumor volume left behind in the surgical bed is a significant predictor of recurrence and survival. In the present study, we analyzed the grade of malignancy in the surgical edge (SE) after tumor removal and correlated it with progression-free survival (PFS) in patients diagnosed with ATDG.

METHODS:
Surgical edge biopsy samples were obtained after tumor removal in 15 patients (3 A2; 2 A3; 2 A4; and 8 GBM, WHO CNS 5); the samples were analyzed by three neuropathologists and were classified in a GOM (2, 3, or 4). An MRI was performed within 48 hours after surgery in all patients to assess the extent of resection. PFS was obtained from clinical and MRI follow-up. Data analysis was conducted using a nonparametric Spearman rank coefficient correlation test.

RESULTS:
Correlation analysis between the highest grade of malignancy found in the SE and PFS was -0.8080 (95% CI = -0.9359 to -0.4920, p < 0.0001). See Image 1.

CONCLUSIONS:
A strong correlation between the GOM in the SE and PFS was found in ATDG Astrocytomas. See Image 2 and 3.
874 Direct Visualization of Planned Resection Volumes with Microbubble-Enhanced MR-Guided Focused Ultrasound Blood-Brain Barrier Opening and Sodium Fluorescein in Infiltrating Gliomas
Authors: Ahmed Abdul-Kareem , Anastasaidis Pavlos , Gandhi Dheeraj , Woodworth Graeme 
Published: March 2023

INTRODUCTION:
Surgical resection of infiltrating gliomas (IG,Grade II-IV) can be challenging, as many do not consistently enhance on gadolinium-enhanced MRI. Evidence of survival benefit of supratotal resection is mounting. No method exists to preoperatively demarcate a planned surgical resection volume. Such physical demarcation could significantly improve the resection of IGs.

METHODS:
Four patients were treated (3M:1F), with a mean age of 32.5 (29-36). All were suspected of having or were previously diagnosed with a supratentorial IG. Patients underwent awake transcranial MB-FUS BBBO of the lesion bed and perilesional areas planned for resection at the discretion of the operating neurosurgeon (GFW). Subsequently, patients were transported directly to the operating room. For each patient, sodium fluorescein was administered, and then tumors were resected with standard craniotomies under general anesthesia, using neuronavigation and fluorescent microscopy.

RESULTS:
Patients tolerated the procedure well without adverse events with a median follow-up of 15.7 months. The mean tumor volume was 18.2 cm3 (6.8-32.9). The demarcated planned resection volume was visualized with fluorescent microscopy. Fluorescing tissue was completely resected. Gross-total or supratotal resection was achieved in all patients. Pathologies included diffuse astrocytoma, oligodendroglioma, and anaplastic oligodendroglioma.

CONCLUSIONS:
MB-FUS BBBO and sodium fluorescein present a safe and novel method of demarcating a preoperatively planned surgical resection volume with resultant intraoperative visualization in IGs. This method is resilient to brain shift, and is an addition to the current repertoire for visualizing non-contrast-enhancing gliomas. The same method can be investigated for use in epilepsy surgery.
879 Monitoring Flash Visual Evoked Potentials During Endoscopic Skull Base Surgery Reduces the Risk of Visual Pathway Injury
Authors: Ochoa-Sanchez; Mazen Alotaibi Rafael , Kilty Shaun , Agbi Charles B. , Lamothe Andre , Schramm David , Alkherayf Fahad A. 
Published: March 2023

INTRODUCTION:
Minimally invasive endoscopic endonasal skull base surgery offer safer and more effective access to the skull base to remove benign and malignant tumors. However, endoscopic skull base surgery around the visual pathway may increase the risk of injury of the visual pathway. Monitoring of flash visual evoked potentials (FVEPs) during surgery may detect a possible injury to the visual pathway, allowing the surgeon to take corrective measures during surgery to reverse or minimize it.

METHODS:
We identified patients with chiasmatic or pre-chiasmatic lesions who underwent minimally invasive endoscopic skull base surgery. Patients were subjected to eye exam (color vision, visual acuity, and visual field evaluation) within 3 months before surgery, and within 6-12 weeks and 6-9 months after surgery to explore short- and long-term surgical outcomes on eyesight. During surgery, FVEPs were recorded from electrodes placed in the scalp overlying the visual cortex after flashing red light stimulation (Cadwell LED stimulating goggles), as reported previously. We examined the relationship between FVEPs amplitude change and visual outcome on these patients.

RESULTS:
102 patients have been enrolled in this retrospective study. The median age at admission was 57 years (range: 22-86 years) and 68.4% of the patients were female. Most of the patients had either pituitary adenoma (64.7%) or skull base meningioma (23.5%). Preliminary eye evaluations (pre- and post-surgery) showed that the patients did not develop short- or long-term eyesight impairments.

CONCLUSIONS:
Our study provides a new method for rapidly acquiring reproducible FVEP waves for which allows for timely reporting significant FVEP changes resulting in prompt signal action. Thus, monitoring FVEPs during endoscopic endonasal skull base surgery reduces the risk of visual pathway injury.
880 Electrophysiological Patterns of Glioma-Induced Neuronal Network Remodeling are a General Property of Brain Tumors Regardless of Subtype
Authors: Aabedi Alexander Arash , Lipkin Benjamin , Young Jacob , Hinkley Leighton , Findlay Anne , Daniel Andy , Krishna Saritha , Umbach Gray , Surapaneni Akhil , Kaur Jasleen , Berger Mitchel , Molinaro Annette , Brang David , Nagarajan Srikantan S. , Hervey-Jumper Shawn L. 
Published: March 2023

INTRODUCTION:
Recent evidence indicates that diffuse gliomas engage with neurons at the single-unit and circuit level through differing mechanisms. Certain malignant gliomas form glioma-neuron excitatory glutamatergic synapses and modulate neuron-neuron synapses through activity-dependent paracrine signaling, while others establish glioma-glioma connections via tumor microtubes. It is therefore possible that diffuse gliomas remodel neuronal circuits in a defined and predictable manner and demonstrate distinct electrophysiological profiles with prognostic and therapeutic significance.

METHODS:
Following spatial-temporal registration, an elastic net logistic regression classifier was used to distinguish between power spectra arising from glioma-remodeled cortex and within-subject control conditions (N = 140). Model significance was determined non-parametrically by re-training each model 1,000 times with randomly permuted class labels and testing the true phi coefficient against the null distribution.

RESULTS:
In the discovery dataset, glioma infiltration was accurately classified based on tumor intrinsic neuronal activity (p < 0.05) in 127 patients (90.7%). Thirty electrophysiological features were identified, which revealed increased power in the delta range (1-4 Hz) and decreased power in the beta range (12-20 Hz) as a unique signature of glioma remodeling (p < 0.05) which was preserved in the validation dataset as well as across WHO 2021 diffuse glioma subtypes. To identify gene expression programs and signaling mechanisms that may contribute to glioma-induced remodeling but are potentially not identified in the current clinical classification scheme, targeted, next generation sequencing was performed and used as covariates, which again demonstrated the significance of the delta-beta spectral features.

CONCLUSIONS:
These data support converging mechanisms of glioma-induced neuronal network remodeling across tumor subtypes, setting the stage for novel therapies such as neuromodulation.
886 Stereotactic Radiosurgery Reprograms Macrophage, Microglia, and T Cell Populations in the Glioblastoma Immune Microenvironment
Authors: Young Jacob , Cho Nam Woo , Lucas Calixto-Hope , Seo Kyounghee , Santos Raquel , Phillips Joanna , Ozawa Tomoko , Berger Mitchel , Raleigh David R. 
Published: March 2023

INTRODUCTION:
Daily ionizing radiation, the first-line treatment for glioblastoma, prevents durable immune infiltration of the tumor microenvironment. Hypofractionated radiotherapy is used to treat glioblastomas at recurrence, but the impact of hypofractionated radiotherapy on the glioblastoma immune microenvironment is incompletely understood.

METHODS:
Syngeneic GL261 (3x105 cells/mouse) or SB28 glioblastoma cells (3 x 104 cells/mouse) were implanted into the frontal lobe of immunocompetent C57BL/6J mice. Bioluminescence was used to assess glioblastoma growth. After tumor engraftment, glioblastomas were treated with conformal SRS (18Gy/1Fx) or sham. Glioblastomas were collected for histologic, single-cell, or molecular analyses 5 days after treatment or at the time of euthanasia after monitoring for survival. Glioblastoma immune microenvironment responses were assessed using (1) H&E, (2) single cell mass cytometry (CyTOF) or IHC to define or validate immune cell changes, respectively, or (3) multiplexed cytokine assays to elucidate molecular mechanisms reprograming the glioblastoma immune microenvironment in response to SRS.

RESULTS:
SRS attenuated glioblastoma growth and prolonged survival compared to sham treatment in both immunocompetent intracranial mouse models (GL261: 14 days versus 27 days, p < 0.001, SB28: 19 days versus 22 days, p = 0.001). CyTOF showed SRS decreased immunosuppressive macrophage infiltration and increased microglia or CD8+ T cell infiltration of the glioblastoma immune microenvironment in GL261 tumors , however, there were very few changes in the lymphoid compartment of SB28 tumors . Histologic analyses validated T cell and microglia infiltration after SRS . Glioblastoma cytokine analysis revealed inhibition of pro-tumor/anti-inflammatory cytokines (IL6, LIF) after SRS in SB28 tumors .

CONCLUSIONS:
Single-fraction SRS reprograms glioblastoma macrophage, microglia, and CD8+ T cell populations in preclinical models, suggesting SRS or inhibition of pro-tumor/anti-inflammatory mechanisms underlying the immunosuppressive glioblastoma microenvironment represent immunogenic therapies that may offer a benefit to patients with glioblastoma.
887 Mizuho Minimally Invasive Brain Tumor Surgery Award
Authors: Yoon Wan Soo 
Published: March 2023

INTRODUCTION:
Early descent of the diaphragm sellae (DS) during endoscopic endonasal transsphenoidal surgery (EETS) for pituitary macroadenoma surgery is occasionally a troublesome event by blocking the surgical field. This can block access for remnant tumor or cause cerebrospinal fluid leak due to uneventful rupture of the DS. Some methods are reported previously, but they have many limitations.

METHODS:
We designed a simple and rigid pituitary retractor with the least space occupation in the nasal cavity to be compatible in EETS. The pituitary retractor was held by external holder system to support the herniated DS stably. We retrospectively reviewed a clinical 22 cases of pituitary macroadenomas underwent EETS using the pituitary retractor.

RESULTS:
The pituitary retractor stably pushed up the herniated DS in all cases, and the surgeon proceeded the procedure with bimanual maneuver. The pituitary retractor was helpful to remove tumors around the medial cavernous sinus and behind the DS in 16 and seven cases, respectively. In four cases, the meticulous hemostasis was completed with the direct visualization by the DS elevation with this retractor. Gross total tumor resection was performed in 20/22 patients (91%). The impaired visual function and hypopituitarism were improved in 18/20 (90%) and 7/14 (50%) patients after surgery, respectively. There was no complication related with the pituitary retractor.

CONCLUSIONS:
During EETS for pituitary macroadenomas, the novel pituitary retractor reported in this study is a very useful technique when the herniated DS block the surgical field and bimanual maneuver. This pituitary retractor can help to result in the excellent surgical outcomes with minimal morbidity.
889 Significance of Cortico-Bulbar Motor Evoked Potential (CoMEP) Monitoring in Vestibular Schwannoma Surgery for Prediction of Postoperative Facial Nerve Palsy
Authors: Roessler Karl , Shawarba Julia , Strasser Elisabeth , Hirschmann Dorian , Hertha Johannes , Reitbauer Stefan , Novak Klaus 
Published: March 2023

INTRODUCTION:
Direct facial nerve stimulation during vestibular schwannoma surgery (VSS) allows preservation of the nerve in its continuity. However, prognostication of post- operative nerve function remains challinging.

METHODS:
In total, 51 patients (median age 52yrs; 19-84) with unilateral VS (median size 20mm;10-50) were included. Intraoperative neuromonitoring consisted of auditory evoked potentials (AEPs), direct facial nerve stimulation and CoMEPs. An anodal stimulation via subdermal corkscrew electrodes on position C3 or C4 and a cathode near Cz with a train of 6 pulses, each with a duration ono 500us, at a frequency of 5000 Hz and an interstimulus-interval of 2 ms was used.

RESULTS:
Complete resection was achieved in >90% of patients. Preoperative hearing was preserved in 78% (40/51) patients and postoperative serviceable hearing was preserved in 35% (18/49) patients and was closely related to preserved wave V of the AEPs. Additionally, MEPs were successfully monitored in every patient for the entire duration of the surgery. In 16% of the patients, more than 50% reduction of MEP baseline amplitude was recorded, correlating directly with moderate to severe postoperative facial palsy (House Brackman 3-5). No facial palsy without MEP amplitude reduction was observed. During 6 months FU, recovery of 1-2 HB Grades was observed in all cases. All patients with intraoperative MEP amplitude reduction had significant larger VS than patients without MEP amplitude reduction (27 vs 22 mm, p = 0.02)

CONCLUSIONS:
In addition to direct facial nerve stimulation, intraoperative CoMEP monitoring during VSS seems to be of high significance and value for intraoperative facial nerve preservation and prognosis estimation of postoperative short- and long-term facial palsy.
892 Validation of Docetaxel as a Radiosensitizer in High-Risk Meningiomas Based on DNA Methylation Profiling
Authors: Youngblood Mark W. , Tran Anh , Wang Wenxia , An Shejuan , Scholtens Denise , Zhang Lyndsee , O’Shea; Jenny Pokorny Kaitlyn , Magill Stephen , Sachdev Sean , Lukas Rimas Vincas , Ahmed Atique , Unruh Dusten , Walshon Jordain , McCortney Kathleen , Wang Yufen , Baran Aneta , Sahm Felix , Aldape Kenneth , Chandler James P. , James David , Heimberger Amy B. , Horbinski Craig 
Published: March 2023

INTRODUCTION:
Genome-wide methylation profiling has emerged as a transformative prognostic tool in meningiomas, and has identified discrete epigenetic clusters that share distinct clinical and biological features. The association of these signatures with underlying pathological mechanisms has not been well-explored.

METHODS:
From a collection of over two-hundred well-annotated tumors, we found 981 genes in which methylation signal was predictive of recurrence. Using pathway enrichment analysis, Docetaxel was identified as a candidate therapeutic agent; a medication that is frequently utilized in other common malignancies. This chemotherapy was validated in vitro and in vivo using patient derived meningiomas, and also tested in combination with radiotherapy.

RESULTS:
Based on 15 patient-derived cultures from our institution and 2 public cell lines, we found that Docetaxel could inhibit meningioma growth at concentrations that were favorable relative to other cancers. This drug triggered apoptosis in meningiomas of all tumor grades and methylation classes, with IC50 values ranging from 0.8 nm to 4.4 nm in vitro. Combining Docetaxel with radiation therapy resulted in synergistic cytotoxic effects on meningioma cultures, associated with elevated markers of cell death and dsDNA breaks. We confirmed our results via orthotopic meningioma models of the cell lines CH157 and IOMM-Lee, which exhibited dose-dependent improvements in mortality and tumor volume in response to Docetaxel and radiation.

CONCLUSIONS:
Our results leverage DNA methylation patterns to repurpose FDA-approved medications in the treatment of meningiomas, and suggest a clinical trial of Docetaxel with radiotherapy may be efficacious in aggressive lesions.
893 Development of a Rabbit Human Glioblastoma Model for Testing of Endovascular Selective Intra-Arterial Infusion (ESIA) of Novel Stem Cell-Based Therapeutics
Authors: Srinivasan Visish M. , Kan Peter , Gumin Joy , Collins Dalis E. , Chen Stephen , Chen Melissa M. , Garcia Robert , Fueyo Juan , Lang Frederick F. 
Published: March 2023

INTRODUCTION:
Poor outcomes for glioblastoma (GBM) are partly due to the inability to deliver therapeutic agents to the tumor because of the blood brain barrier (BBB) as well as ineffective agents. We have evaluated bone marrow-derived mesenchymal stem cells (MSCs) as transport vehicles of biological therapeutics, particularly Delta-24-RGD (D24), an engineered tumor-selective oncolytic adenovirus that replicates and lyses only in human tumor cells. In mice, we have shown that MSCs loaded with D24 (MSCs-D24) home to GBMs after intra-arterial (IA) carotid infusion, resulting in widespread distribution in the tumor and oncolysis. Intravenous infusion is ineffective, rendering IA delivery the only effective systemic option. To understand the applicability of IA infusions of MSCs-D24 requires a large animal, human brain tumor model recapitulating the challenges of clinical use.

METHODS:
Rabbits underwent stereotactic xenoimplantation of human GBM cell lines. Tumor creation was confirmed on magnetic resonance imaging (MRI), histologic and immunohistochemistry analysis. Selective internal carotid artery MSCs-D24 infusion ipsilateral to the tumor was performed assess efficacy and safety.

RESULTS:
We report on successful xenoimplantation of human glioblastoma cell lines (U87, GSC17, and GSC8-11) in 28 immunosuppressed rabbits using Mycophenolate Mofetil, Dexamethasone, and Tacrolimus. The implanted rabbits were followed for 35 days prior to tumor assessment with MRI, angiography, and histological analysis. On MRI, the tumors were hyperintense on T2-weighted image and enhanced (evidence of BBB breakdown). On histological analysis, tumors showed phenotypic traits of human GBM and display varying levels of vascularity, an important feature for testing IA therapy. Selective internal carotid artery MSCs-D24 infusion was safe in the model, and MSCs-D24 homed to the implanted tumor at 24 hours.

CONCLUSIONS:
The intracranial immunosuppressed rabbit human GBM model allows testing of ESIA infusion of novel therapeutics (eg. MSCs-D24) in a clinically relevant fashion.
Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing
Authors: Karlsen Anders , Yeung Ching-Yan Chloé , Schjerling Peter , Denz Linda , Hoegsbjerg Christian , Jakobsen Jens R. , Krogsgaard Michael R. , Koch Manuel , Schiaffino Stefano , Kjaer Michael , Mackey Abigail L. 
Published: March 2023

ABSTRACT
The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre that is faced with the challenge of maintaining robust cell–matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24,124 nuclei in semitendinosus muscle–tendon samples from three healthy males by using single-nucleus RNA sequencing (snRNA-seq), alongside spatial transcriptomics, to gain insight into the genes characterizing this specialization in humans. We identified a cluster of MTJ myonuclei represented by 47 enriched transcripts, of which the presence of ABI3BP, ABLIM1, ADAMTSL1, BICD1, CPM, FHOD3, FRAS1 and FREM2 was confirmed at the MTJ at the protein level in immunofluorescence assays. Four distinct subclusters of MTJ myonuclei were apparent, comprising two COL22A1-expressing subclusters and two subclusters lacking COL22A1 expression but with differing fibre type profiles characterized by expression of either MYH7 or MYH1 and/or MYH2. Our findings reveal distinct myonuclei profiles of the human MTJ, which represents a weak link in the musculoskeletal system that is selectively affected in pathological conditions ranging from muscle strains to muscular dystrophies.
Diet in the prevention and treatment of depression - systematic review
Authors: Szulc Izabela , Swacha Weronika , Kozłowska Julia , Pacek Katarzyna , Piekarska Małgorzata , Kasperski Radosław , Jedlina Klaudia , Rusin Klaudia , Budziłło Julia , Lewandowska Monika 
Published: March 2023

Depression is one of the leading causes of disability worldwide. It affects, according to WHO, nearly 300 million people worldwide, a number close to 3,8 % of the world’s population. Depression is not exclusively a mental disorder limited to psychiatry, nowadays it is present in all medical fields. It has a significant association with many comorbidities, as an example cardiovascular diseases and metabolic disorders. Additionally, depression often is manifested with psychosomatic symptoms. Both aspects lead clinicians to major diagnostic and therapeutic challenges. Each one of dietary element: omega-3 fatty acids, zinc, selenium, iron, vitamin B12, vitamin D, folate, creatine was found to correspond with mental health in patients and the level of mentioned diet components is negatively correlated with depression risk of individuals. Each of the dietary aspect considered in the article has a significant correlation with depression disorder and might support the new multidisciplinary attitude toward it in mental health.
Store-Wide Shelf-Space Allocation with Ripple Effects Driving Traffic
Authors: Flamand Tülay , Ghoniem Ahmed , Maddah Bacel 
Published: March 2023

How Product Locations Drive Traffic Throughout a Retail Store In “Store-Wide Shelf-Space Allocation with Ripple Effects Driving Traffic,” Flamand, Ghoniem, and Maddah develop a framework for deciding where to place products in a store, in addition to apportioning the shelf space among products, in a way that maximizes impulse profit, a phenomenon that may account for 50% of transactions. By analyzing a large data set of customer receipts from a grocery store in Beirut, the authors develop a regression model that estimates traffic at a shelf based on its location and the “attraction” from products allocated nearby. The traffic model is embedded within a mixed-integer nonlinear program, which they solve via specialized linear approximations. For the store in Beirut, a 65% improvement in impulse profit is anticipated, and the location of products is found to be significantly more important in driving store-wide traffic than the relative shelf-space allocation.
A cautionary tale of two canines: a case report
Authors: Hoare Sophie , Larmour Colin 
Published: March 2023

This orthodontic case report highlights the unpredictable behaviour of ectopic maxillary permanent canines. An 11-year-old male patient had bilateral ectopic maxillary canine teeth that were in high positions and initially left in situ and monitored. During orthodontic treatment, there was rapid spontaneous eruption of both teeth with resulting resorption to an adjacent lateral incisor. This case report details the clinical findings and progress of treatment and management. A review of the literature reinforces the importance of maintaining regular radiographic review to assess for migration of ectopic teeth, and to ensure appropriate treatment planning and assessment of potential complications. CPD/Clinical Relevance: This report highlights the need for regular radiographic review of impacted canines in younger patients.