bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring vimentin's role in breast cancer via PICK1 alternative polyadenylation and the miR‐615‐3p‐PICK1 interaction
Authors: Jia Xinyan , Shao Lujing , Quan Hong , Zhong Zhixian , Dong Chunyan 
Published: January 2025

AbstractBreast cancer continues to be a major health issue for women worldwide, with vimentin (VIM) identified as a crucial factor in its progression due to its role in cell migration and the epithelial‐to‐mesenchymal transition (EMT). This study focuses on elucidating VIM's regulatory mechanisms on the miR‐615‐3p/PICK1 axis. Utilizing the 4T1 breast cancer cell model, we first used RNA‐seq and proteomics to investigate the changes in the APA of PICK1 following VIM knockout (KO). These high‐throughput analyses aimed to uncover the underlying transcriptional and proteomic alterations associated with VIM's influence on breast cancer cells. RNA‐seq and proteomic profiling revealed significant APA in PICK1 following VIM KO, suggesting a novel mechanism by which VIM regulates breast cancer progression. Validation experiments confirmed that VIM KO affects the miR‐615‐3p‐PICK1 axis, with miR‐615‐3p's regulation of PICK1 being contingent upon the APA of PICK1. These findings highlight the complex interplay between VIM, miR‐615‐3p, and PICK1 in the regulation of breast cancer cell behavior. This study reveals that vimentin affects the miR‐615‐3p‐PICK1 axis through APA, revealing the key role of VIM in cancer progression. Opened up new avenues for targeted cancer therapy, with a focus on regulating the interaction between APA and miR‐615‐3p‐PICK1.
Echinacoside promotes collagen synthesis and survival via activation of IGF‐1 signaling to alleviate UVB‐induced dermal fibroblast photoaging
Authors: Wen Su‐Ying , Ng Shang‐Chuan , Noriega Lloyd , Chen Tzu‐Jung , Chen Chih‐Jung , Lee Shin‐Da , Huang Chih‐Yang , Kuo Wei‐Wen 
Published: January 2025

AbstractUltraviolet (UV) irradiation is a major factor contributing to skin photoaging, including the formation of reactive oxygen species (ROS), collagen breakdown, and overall skin damage. Insulin‐like growth factor‐I (IGF‐1) is a polypeptide hormone that regulates dermal survival and collagen synthesis. Echinacoside (Ech), a natural phenylethanoid glycoside, is the most abundant active compound in Cistanches. However, its potential benefits for the skin and the underlying molecular mechanisms remain unclear. The objective of this research is to investigate the protective effect of Ech on human dermal fibroblast cells (HDFs) against UVB‐induced skin photodamage. In this study, we demonstrated that Ech promotes IGF‐1/IGF‐1R/ERK‐mediated collagen synthesis and IGF‐1/IGF‐1R/PI3K‐mediated survival pathways, as well as induces IGF‐1 secretion to counteract UVB‐induced aging in HDFs. Furthermore, UVB‐induced accumulation of SA‐β‐gal‐positive cells, ROS, and impaired collagen synthesis were attenuated following Ech treatment. However, the protective effects of Ech were significantly diminished when IGF‐1 and IGF‐1R expression was silenced using small interfering RNA, indicating that Ech exerts its antiaging effects primarily by activating the IGF‐1/IGF‐1R signaling pathway. Our findings provide evidence of the antiaging effects of Ech on UVB‐induced skin photodamage and suggest its potential development as a supplement in cosmetic dermal protective products.
FAM49B drives colorectal cancer progression by stabilizing c‐Myc through NEK9 phosphorylation
Authors: Lu Chen , Liu Tianyu , Yimin E. , Miao Lin , Yu Chunzhao , Zhang Jianping , Luo Xiagang 
Published: January 2025

AbstractColorectal cancer (CRC) ranks as the third most prevalent cancer globally and is the second leading cause of cancer mortality. FAM49B, a member of the FAM49 gene family, is a recently identified, evolutionarily conserved gene. Emerging studies indicate that FAM49B plays a role in various cancers, though its specific mechanism in CRC remains largely unexplored. In this study, we observed that FAM49B was abnormally expressed in CRC tissues and cell lines, with elevated expression correlating with poor patient prognosis. FAM49B knockdown markedly suppressed CRC cell proliferation by arresting the cell cycle and reducing cell migration and invasion. Single‐cell RNA‐seq (ScRNA‐seq) analysis revealed that high FAM49B expression in malignant epithelial cell clusters was strongly linked to c‐Myc oncogene activation. Further, FAM49B knockdown significantly reduced c‐Myc expression by enhancing its K48 ubiquitination. We identified NEK9 as a direct interacting partner of FAM49B, with FAM49B knockdown inhibiting NEK9‐Thr210 phosphorylation. Similarly, high NEK9 expression was linked to unfavorable prognosis in CRC. In FAM49B‐overexpressing CRC cells, NEK9 knockdown significantly suppressed c‐Myc expression, c‐Myc‐ser62 phosphorylation, and reduced cell proliferation, migration, and invasion. Thus, directly targeting the FAM49B/NEK9/c‐Myc pathway presents a promising therapeutic approach for c‐Myc positive CRC patients.
Effects of Mental Workload Manipulation on Electroencephalography Spectrum Oscillation and Microstates in Multitasking Environments
Authors: Li Wenbin , Cheng Shan , Dai Jing , Chang Yaoming 
Published: January 2025

ABSTRACTIntroductionMultitasking during flights leads to a high mental workload, which is detrimental for maintaining task performance. Electroencephalography (EEG) power spectral analysis based on frequency‐band oscillations and microstate analysis based on global brain network activation can be used to evaluate mental workload. This study explored the effects of a high mental workload during simulated flight multitasking on EEG frequency‐band power and microstate parameters.MethodsThirty‐six participants performed multitasking with low and high mental workloads after 4 consecutive days of training. Two levels of mental workload were set by varying the number of subtasks. EEG signals were acquired during the task. Power spectral and microstate analyses were performed on the EEG. The indices of four frequency bands (delta, theta, alpha, and beta) and four microstate classes (A–D) were calculated, changes in the frequency‐band power and microstate parameters under different mental workloads were compared, and the relationships between the two types of EEG indices were analyzed.ResultsThe theta‐, alpha‐, and beta‐band powers were higher under the high than under the low mental workload condition. Compared with the low mental workload condition, the high mental workload condition had a lower global explained variance and time parameters of microstate B but higher time parameters of microstate D. Less frequent transitions between microstates A and B and more frequent transitions between microstates C and D were observed during high mental workload conditions. The time parameters of microstate B were positively correlated with the delta‐, theta‐, and beta‐band powers, whereas the duration of microstate C was negatively correlated with the beta‐band power.ConclusionEEG frequency‐band power and microstate parameters can be used to detect a high mental workload. Power spectral analyses based on frequency‐band oscillations and microstate analyses based on global brain network activation were not completely isolated during multitasking.
Decreased Choroidal Vascular Index in Idiopathic Intracranial Hypertension
Authors: Wang Hang , Wang Rui , Cao Le , Kwapong William Robert , Liu Ruishan , Hu Fayun , Wu Bo 
Published: January 2025

ABSTRACTIntroductionWe aimed to explore the difference in choroidal vascular parameters using swept‐source optical coherence tomography (SS‐OCT) in patients with idiopathic intracranial hypertension (IIH) compared to controls. We also explored the ability of the choroidal parameters to reflect elevated intracranial pressure (ICP) in patients with IIH.MethodsThis observational study recruited patients diagnosed with IIH and healthy controls. A lumbar puncture was performed for ICP measurement. All the participants underwent OCT examinations. The choroid was automatically segmented and imaged by the OCT tool. The parafoveal choroidal vascular volume (CVV) and choroidal vascular index (CVI) were calculated in 3 mm annulus and 6 mm annulus.ResultsA total of 80 patients with IIH (34.67 ± 11.00 years; 37.50% males) and 92 controls (34.50 ± 12.08 years; 36.96% males) were included in the final analysis. Patients with IIH had higher BMI (< 0.001) and poor visual acuity (< 0.001) compared with controls. Patients with IIH demonstrated significantly lower parafovea CVI in both annuluses (p = 0.003 for 3 mm annulus, p = 0.001 for 6 mm annulus) compared to controls. Mean parafovea CVI in both annuluses was significantly correlated with ICP level (p = 0.014 for 3 mm annulus, p = 0.015 for 6 mm annulus). The combination of CVV and CVI in a 6 mm annulus demonstrated the highest diagnostic value with a mean AUC of 0.818.ConclusionCVI may serve as a potential marker for identifying IIH and reflecting ICP changes.
Lesion Topography and Clinical Features Associated With Respiratory Failure in Patients With Medullary Infarction
Authors: Zhao Na , Liu Ran , Zhang Yajing , Ling Ling , Zhang Chao , Zhang Ting , Yue Wei 
Published: January 2025

AbstractBackgroundRespirator failure (RF) is a severe malignant complication in both lateral medullary infarction (LMI) and medial medullary infarction (MMI) patients. However, the differences in clinical and radiological manifestations associated with RF between patients with LMI and MMI have not been fully elucidated.MethodsA total of 435 consecutive patients with MI within 7 days of onset in our institute were retrospectively enrolled from January 2017 to January 2024. Lesions were categorized both rostral‐caudally and horizontally, and clinical characteristics were collected to evaluate the correlation between them and RF that occurred within 10 days of stroke onset.ResultsAmong the 435 patients, 33 patients developed RF, with 19 exhibiting LMI and 12 exhibiting MMI. Multisegment involvement was more common among LMI patients experiencing RF compared to those without (52.6% vs. 23.9%, p = 0.012). However, this difference was not observed among MMI patients. Large (n = 12, 63.2%, p = 0.014) and typical (n = 6, 31.6%, p = 0.016) lesions were more common among LMI patients with RF. In MMI patients with RF, nine (75.0%) patients had long lesions extending from the ventral to the dorsal medulla, with six of these cases involving bilateral lesions, as V‐shape or heart‐shape on MRI. Univariate analysis of clinical symptoms revealed that bulbar symptoms, urinary retention, and pulmonary infection were significantly more common in both the LMI and MMI groups with RF.ConclusionsSpecific lesion patterns, such as large and typical infarctions in LMI patients or long lesions, particularly those with bilateral infarctions, as V‐shape or heart‐shape on MRI in MMI patients, appear to correlate with a higher incidence of RF, while clinical symptoms associated with RF are similar in LMI and MMI.
Pretreatment Fatigue in Breast Cancer Patients: Comparison With Healthy Controls and Associations With Biopsychosocial Variables
Authors: Blickle Patricia , Schmidt Martina E. , Steindorf Karen 
Published: January 2025

ABSTRACTObjectiveCancer‐related fatigue is one of the most common burdens of cancer patients. To date, most studies focused on fatigue during or after treatment. However, investigation of pretreatment fatigue is crucial to identify causal or risk factors other than cancer therapy and to enable timely fatigue management.MethodsTwo hundred and thirty‐two breast cancer patients (mean age = 55.6) and 41 healthy participants (mean age = 49.3) were recruited via the National Center for Tumor Diseases (NCT) Heidelberg. Patient‐reported outcomes were assessed with the EORTC QLQ‐FA12 for fatigue, the EORTC QLQ‐C30 for functioning, the STAI for anxiety, the CESD‐R for depression and the PSQI for sleep disturbance. Descriptive analyses and logistic regression models were performed using baseline data before start of cancer treatment. The thresholds of clinical importance (TCI) were applied to test for clinically relevant fatigue.ResultsCompared to the healthy participants, patients scored significantly higher in physical, emotional, and total fatigue, in depression, in global health status and in all functioning scales except cognitive function (all p < 0.01). 48.7% of all patients reported clinically relevant fatigue. Being younger, being obese, having low education, or low social support was associated with a higher likelihood of clinically relevant fatigue before treatment. Higher depression and anxiety scores, poorer sleep quality and global health status, and impaired functioning seemed to get along with an increased likelihood of scoring above the TCI of fatigue (all p < 0.001).ConclusionsOur study results suggest that fatigue screening, patient‐centered fatigue education and psychosocial support may be needed already from the time of cancer diagnosis.
Does the School Environment and School Engagement in Early High School Predict Trajectories of Anti‐Social Behaviour? A National Longitudinal Study of Australian Youth From 12 to 19 years
Authors: Halls Olivia , Edwards Ben 
Published: January 2025

ABSTRACTBackgroundSchools are widely considered important agents of social control for young people. Consequently, school engagement, disengagement and the school environment are key to understanding behavioural outcomes during adolescence. This study addresses an empirical gap in longitudinal research by examining new types of school engagement, as well as school‐level environmental factors.AimsTo understand the role that school engagement and the school environment play in shaping trajectories of antisocial behaviour.MethodsUsing longitudinal survey of Australian children (LSAC), this study employed trajectory analysis to identify trajectories of ASB over 4 waves. We tested the influence that early high school engagement and the school environment had on ASB trajectories through a series of multinomial logistic regressions.ResultsOf the 2983 Australian school students included in the study, three trajectory groups were identified: no ASB (n = 1599), low‐level ASB (n = 1158) and moderate‐level ASB (n = 88). The influence of truancy, suspension/expulsion and school avoidance were all particularly strong, and student–teacher relationships were found to both directly and indirectly influence levels of ASB.ConclusionsThis longitudinal study provides a comprehensive overview of trajectories of antisocial behaviour during adolescence and how they are influenced by experiences at school. It confirms the strong influence of behavioural disengagement and offers new insights into the role of affective school engagement and the school environment.
Comparative Outcomes of Supera Interwoven Nitinol Versus Eluvia Fluoropolymer‐Based Drug‐Eluting Stents for the Treatment of Severely Calcified Femoropopliteal Artery Lesions: Results of the ELDORADO Study
Authors: Yanagiuchi Takashi , Tokuda Takahiro , Yoshioka Naoki , Kojima Shunsuke , Tanaka Akiko , Takei Tatsuro , Ogata Kenji , Yamaguchi Kohei , Nakama Tatsuya , Yokoi Hirokazu ,   LEADers PAD investigators
Published: January 2025

ABSTRACTBackgroundSupera interwoven nitinol stents (IWNS) and Eluvia fluoropolymer‐based drug‐eluting stents (DES) were designed to improve the patency of the femoropopliteal (FP) artery; however, which type of stent yields superior outcomes in calcified FP lesions remains unclear.AimsTo compare the safety and efficacy of Supera IWNS and Eluvia DES in severely calcified FP lesions.MethodsThis study retrospectively analyzed 257 consecutive patients who underwent endovascular therapy using either IWNS (n = 123) or DES (n = 134) for FP lesions with peripheral arterial calcium scoring system (PACSS) grade 3 or 4 severe calcification between April 2018 and December 2021 at eight cardiovascular centers in Japan.ResultsPropensity score (PS) matching extracted 138 matched patients with no remarkable intergroup difference in patient and lesion characteristics. The 1‐year primary patency rates in the matched population were not significantly different between the IWNS and DES groups (85.4% vs. 89.8%, p = 0.320). A significant interaction between the stents used and the number of below‐the‐knee (BTK) runoff vessels was observed (interaction p = 0.048). The hazard ratio for restenosis was 2.68 (95% confidence interval, 0.51–14.2) in the group with no BTK runoff, favoring DES.ConclusionIn PS‐matched patients with severely calcified FP lesions, 1‐year primary patency was not significantly different between treatments using Supera IWNS and Eluvia DES.
Methane to Methanol Transformation on Cu2+/H‐ZSM‐5 Zeolite. Characterization of Copper State and Mechanism of the Reaction
Authors: Gabrienko Anton A. , Kolganov Alexander A. , Yashnik Svetlana A. , Kriventsov Vladimir V. , Stepanov Alexander G. 
Published: January 2025

AbstractCu‐modified zeolites provide methane conversion to methanol with high selectivity under mild conditions. The activity of different Cu‐sites for methane transformation is still under discussion. Herein, ZSM‐5 zeolite has been loaded with Cu2+ cations (1.4 wt % Cu) as characterized by UV‐vis DRS, EPR, EXAFS, and 1H MAS NMR. It is inferred that Cu2+ cations, attached to the cation‐exchange Al−O−−Si sites of the zeolite framework, can exist in the form of either isolated or paired Cu2+ sites. The transformation of methane to methanol on Cu2+/H‐ZSM‐5 has been verified by the observation of the methoxy species formation with 13C MAS NMR and FTIR spectroscopy. The related mechanisms have been analyzed by DFT calculations. The calculations show that the paired Cu2+ sites enable heterolytic C−H bond dissociation via the “alkyl” pathway resulting in methylcopper species, which however are not detected experimentally due to further rapid transformation to surface methoxy species through methyl radical formation and recombination with Si−O−Al site. Based on the obtained data, it has been concluded that methane transformation to methanol on paired Cu2+ sites, having no extra‐framework oxygen ligand, is possible in Cu‐modified zeolites. The pathways of Cu2+ cations regeneration with O2 and H2O have been experimentally explored.
Solvent‐Dependent C(sp3)−CF3 Reductive Elimination from Neutral Four‐Coordinate Cu(III) Complexes
Authors: Weng Yuecheng , Pan Deng , Wu Jian , Leng Xuebing , Xue Xiao‐Song , Shen Qilong 
Published: January 2025

AbstractA solvent dependent C(sp3)−CF3 bond‐forming reductive elimination from neutral four‐coordinate Cu(III) complexes [(L)CuIII(CF3)2(CH2CO2tBu)] (L=pyridine or its derivatives) is described. Reactions in less polar solvent ClCH2CH2Cl proceed via a concerted bond breaking/bond forming process along with the reorientation of the ligand, while reaction in polar solvent DMF occurs via a rate limiting ligand‐dissociation, followed by C(sp3)−CF3 reductive elimination from the resulting three‐coordinate intermediate. These mechanistic proposals are supported by kinetic studies that included ligand and temperature effects, as well as DFT calculations.
One‐dimensional Branched PdAg Nanoalloy Rich in Defects for Boosting Glycerol and Ethanol Oxidation
Authors: Tan De‐Xin , Wang Yan‐li , Ning Xiao‐Mei , Luo Xiao‐Li , Chen Chun‐Ru , Xiao Qiao , Chen Jian‐Hong 
Published: January 2025

AbstractThe development of Pd‐based bimetallic nanoalloys (NAs) with abundant active sites for achieving highly efficient electrocatalysis in alcohol oxidation deserves continuous attention. Herein, we utilized a facile visible‐light‐assisted liquid‐phase method involving adjusting reaction time to generate active sites, successfully synthesizing one‐dimensional (1D) PdAg NAs rich in defects. The optimized 1D PdAg NA exhibits remarkable electrochemical activity, stability, and antipoisonous properties in glycerol oxidation reaction (GOR) and ethanol oxidation reaction (EOR), far exceeding pure Pd and commercial Pd/C catalysts. Notably, compared to glycerol oxidation (1496 mA mg−1Pd and 4.13 mA cm−2), the optimized PdAg NA demonstrated higher mass activity (2990 mA mg−1Pd) and specific activity (7.86 mA cm−2) in ethanol oxidation. In summary, this work may provide a strategy for designing low‐cost electrocatalysts enriched with defects, thereby promoting the development of high‐performance alcohol oxidation reactions.
Doubly Linked Azulene Dimer: A Novel Non‐benzenoid Isomer of Perylene
Authors: Hatakenaka Ryoji , Urabe Kazuma , Ueno So , Yamauchi Mitsuaki , Mizuhata Yoshiyuki , Yamada Hiroko , Mikata Yuji , Kamijo Shin , Tani Fumito , Murafuji Toshihiro 
Published: January 2025

AbstractWe report herein the synthesis of an unprecedented isomer of perylene, dicyclohepta[cd,fg]‐as‐indacene bearing two phenyl groups (1‐Ph) by the nickel‐mediated intramolecular homocoupling of a 4,4’‐biazulene derivative (2). The X‐ray crystallographic analysis and theoretical calculations revealed that 1‐Ph adopts a unique helically twisted geometry although the local aromaticity of azulene moieties was preserved. The double covalent linkage of the two azulene skeletons imparts significant orbital interaction, which affords near‐infrared (NIR) absorption (up to 1720 nm) and remarkable redox behaviors despite its closed‐shell electronic structure. The optical band gap of 1‐Ph is calculated to be 0.72 eV from its absorption onset, which is one of the narrowest values among the hitherto reported air‐stable non‐benzenoid PAHs. Furthermore, the thin‐film of 1‐Ph serves as a p‐type semiconductor. Our study offers fundamental insights into not only the aromaticity with the nonalternant topologies of 1‐Ph but also its potential application in novel organic electronics.
New Hydrazone Derivatives Based on Pyrazolopyridothiazine Core as Cytotoxic Agents to Colon Cancers: Design, Synthesis, Biological Evaluation, and Molecular Modeling
Authors: Glomb Teresa , Środa‐Pomianek Kamila , Palko‐Łabuz Anna , Wesołowska Olga , Wikiera Agnieszka , Wojtkowiak Kamil , Jezierska Aneta , Kochel Andrzej , Lesyk Roman , Świątek Piotr 
Published: January 2025

AbstractIn this research, a series of novel hydrazone derivatives based on pyrazolopyridothiazinylacetohydrazide were designed, synthesized, and evaluated for their in vitro cytotoxic potency on several human colon cancer cells (HTC116, HT‐29, and LoVo). After MTT and SRB assays four of the most active derivatives: hydrazide GH and hydrazones GH7, GH8, and GH11, were chosen for further investigation. Hydrazone GH11 had the highest cytotoxic activity (IC50 values of c.a. 0.5 μM). Additionally, the impact of novel derivatives on the oxidative stress level, apoptosis induction, and modulation of inflammation in colon cancer cells was examined. In all studies, the activity of the derivatives increased in order GH < GH7 < GH8 < GH11. At the same time, most of the research was conducted on compounds combined with apple pectin (PC). The most interesting observation was that all the studied derivatives applied together with PC showed significantly higher activity than observed in the case of using PC, hydrazide, or hydrazones separately. Finally, computational chemistry methods (molecular modeling and Density Functional Theory – DFT) were used to complement the experimental studies.