bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of senescence‐associated secretory phenotype and exosome‐shuttled miRNAs by Haritaki fruit extract in senescent dermal fibroblasts
Authors: Bogdanowicz Patrick , Roullet Nicolas , Bensadoun Paul , Bessou‐Touya Sandrine , Lemaitre Jean‐Marc , Duplan Hélène 
Published: March 2023

AbstractObjectiveSkin ageing is linked to the accumulation of senescent cells and a “senescence‐associated secretory phenotype” (SASP). SASP factors include chemokines, cytokines, and small extracellular vesicles (EVs) containing miRNAs. We characterized SASP profile markers in normal human dermal fibroblasts (HDFs) and evaluated the effect of Haritaki fruit extract on these senescence markers.MethodsSenescence was induced in HDFs by ionizing radiation (X ray), followed by 14 days of culture. Parallel incubations included fibroblasts treated for 12 days with 10 or 100 μg/mL Haritaki (a standardized extract of Terminalia chebula fruit). Senescence was assessed on Day 14 according to cell morphology, β‐galactosidase activity, RT‐qPCR measurement of SASP genes, as well as semi‐quantitative (RT‐qPCR) expression of miRNAs contained in EVs isolated from the medium. The size and distribution of EVs were measured by Nanoparticle Tracking Analysis.ResultsHuman dermal fibroblasts exhibited a senescent phenotype 14 days after ionizing‐radiation, demonstrated by a flattened and irregular shape, increased β‐galactosidase activity and over‐expression of SASP genes. CSF3, CXCL1, IL1β, IL6 and IL8 genes were increased by 1492%, 1041%, 343%, 478%, 2960% and 293%, respectively. The cell cycle inhibitor, CDKN1A, was increased by 357%, while COL1A1, was decreased by 56% and MMP1 was increased by 293%. NTA analysis of the EVs size distribution indicated a mix of exosomes (45–100 nm) and microvesicles (100–405 nm). miRNA expression in EVs was increased in senescent fibroblasts. miR 29a‐3p, miR 30a‐3p, miR 34a‐5p, miR 24a‐3p and miR 186‐5p were increased in senescent HDF by 4.17‐, 2.43‐, 1.17‐, 2.01, 12.5‐fold, respectively. Incubation of senescent fibroblasts with Haritaki extract strongly decreased SASP mRNA levels and miRNA expression in EVs.ConclusionHaritaki strongly reduced SASP expression and EV‐shuttled miRNAs in senescent fibroblasts. These results indicate that Haritaki has strong senomorphic properties and may be a promising ingredient for the development of new anti‐ageing dermo‐cosmetic products by inhibiting deleterious effects of senescent cells.
Targeting hexokinase 1 alleviates NLRP3‐mediated inflammation in apical periodontitis: A laboratory investigation
Authors: Qian Yajie , Chen Deyan , Zhu Yanan , Wu Jing , Wang Yong , Yang Weidong 
Published: March 2023

AbstractAimThe aim of the study was to explore whether hexokinase 1 (HK1) is involved in the inhibition of inflammation mediated by nucleotide‐binding oligomerization domain‐like receptor protein 3 (NLRP3) signalling pathway in the development of apical periodontitis (AP).MethodologyHuman AP tissues and normal control tissues were collected in the clinic. First, the levels of glucose, pyruvate, lactate and hexokinase activity were examined in human AP tissues. ECAR and OCR were further measured to detect the level of glycolysis in vitro model of inflammation, which established with lipopolysaccharide (LPS)‐stimulated RAW264.7 cell line. Secondly, the expression of HK1, NLRP3, caspase‐1 and interleukin (IL)‐1β were measured by Western blot, immunohistochemistry or RT–qPCR. Finally, lentiviral short hairpin RNA (shRNA) silencing technique or the inhibitor 2‐deoxy‐d‐glucose (2‐DG) were used to further detect the relationship between HK1‐mediated glycolysis and NLRP3‐mediated inflammation in the development of AP in vitro.ResultsInitially, the level of glycolysis was significantly increased in human AP tissues. Subsequently, the expression of HK1, NLRP3, caspase‐1 and IL‐1β were upregulated significantly in human AP tissues. Furthermore, in the model of AP in vitro, a high level of glycolysis and the high expression of HK1, NLRP3, caspase‐1 and IL‐1β was observed. Finally, the expression of NLRP3, caspase‐1 and IL‐1β mediated by LPS stimulation was significantly reduced via HK1 knockdown or 2‐DG treatment in vitro.ConclusionsOur data support that HK1‐mediated glycolysis plays a crucial role in the development of AP via upregulating the NLRP3 signalling pathway. Moreover, targeting HK1 may contribute to prevent the progression of AP, which has a potential clinical translation.
Preparation of low lactose prebiotic milk using β‐galactosidase from lotus seeds (Nelumbo nucifera)
Authors: Zhong Ailing , Katrolia Priti , Zhu Chong , Kopparapu Narasimha Kumar 
Published: March 2023

SummaryA novel β‐galactosidase (NelnuGal) was isolated from Lotus (Nelumbo nucifera) seeds for the first time in this study. The enzyme was purified to homogeneity using hydrophobic interaction chromatography and ion exchange chromatography. Purified NelnuGal appeared as a monomer with a molecular mass of 50 kDa on SDS‐PAGE gel and zymogram. It was optimally active at pH 4.0 and stable in a broad pH range of 4–10. NelnuGal displayed temperature optimum of 50 °C and thermostability from 20 to 50 °C. It was resistant to inhibition by most of the metal ions tested, especially Ca+2and Mg+2making it useful in hydrolysis of milk. Remarkably, NelnuGal was not inhibited by high concentrations of galactose, which is end‐product of lactose hydrolysis. The purified NelnuGal displayed lactose hydrolysis and transgalactosylation properties. Addition of lotus seed powder to milk and whey resulted in lactose hydrolysis, forming galactose, glucose and galactooligosaccharides. Therefore, the addition of lotus seed powder to milk and whey can be applied for reducing the lactose present in dairy products which will also have potential prebiotic properties.
Specificity of phonological representations in U.S. English‐speaking late talkers and typical talkers
Authors: Curtis Philip R. , Estabrook Ryne , Roberts Megan Y. , Weisleder Adriana 
Published: March 2023

AbstractLate talkers are a heterogeneous group of children who experience delayed language development in the absence of other known causes. Late talkers show delays in expressive phonological development, but less is known about their receptive phonological development. In the current study, U.S. monolingual English‐speaking typical talkers (TTs) (n = 23, mean age = 26.27 months, 57% male; 78.3% White) and late talkers (n = 22, mean age = 24.57 months, 59% male, 72.7% White) completed a Looking‐While‐Listening task to assess their sensitivity to mispronunciations. Results revealed that late talkers and TTs looked to the referent of a word for a shorter duration when it was mispronounced than when it was correctly pronounced, suggesting they were sensitive to mispronunciations. However, there were no significant differences between the two groups in their sensitivity to mispronunciations.
Coronally advanced flap versus tunnel technique for the treatment of peri‐implant soft tissue dehiscences with the connective tissue graft: A randomized, controlled clinical trial
Authors: Tavelli Lorenzo , Majzoub Jad , Kauffmann Frederic , Rodriguez Maria Vera , Mancini Leonardo , Chan Hsun‐Liang , Kripfgans Oliver D. , Giannobile William V. , Wang Hom‐Lay , Barootchi Shayan 
Published: March 2023

AbstractAimTo evaluate the efficacy of coronally advanced flap (CAF) versus tunnel technique (TUN) in covering isolated mid‐facial peri‐implant soft tissue dehiscences (PSTDs).Materials and MethodsTwenty‐eight participants presenting with isolated non‐molar implants exhibiting PSTDs were enrolled and randomized to receive either CAF or TUN, both with a connective tissue graft (CTG). The primary outcome of the study was the percentage of mean PSTD coverage at 12 months. Secondary endpoints included the frequency of complete PSTD coverage, changes in keratinized mucosa width (KMW) and horizontal mucosal thickness (MT), as assessed with transgingival probing, 3D optical scanning and ultrasonography, professional aesthetic evaluation and patient‐reported outcome measures (PROMs).ResultsAt 12 months, the mean PSTD coverage of the CAF and TUN groups was 90.23% and 59.76%, respectively (p = .03). CAF‐treated sites showed a substantially higher frequency of complete PSTD coverage (p = .07), together with significantly greater gain of KMW (p = .01), increase in MT (p = .02), volumetric gain (p < .01) and professional aesthetic outcomes (p = .01). Both interventions showed an improvement in patient‐reported aesthetics and a reduction of the anxiety related to the appearance of the implant compared to baseline, with the CAF group obtaining significantly higher scores (p = .03 for both PROMs).ConclusionsCAF + CTG resulted in superior PSTD coverage outcomes, greater gain in KMW and MT, and better PROMs than TUN + CTG for the treatment of isolated PSTDs (ClinicalTrials.gov NCT03498911).
Auxin signaling module OsSK41‐OsIAA10‐OsARF regulates grain yield traits in rice
Authors: Ma Fuying , Zhang Fan , Zhu Yu , Lan Dengyong , Yan Peiwen , Wang Ying , Hu Zejun , Zhang Xinwei , Hu Jian , Niu Fuan , Liu Mingyu , He Shicong , Cui Jinhao , Yuan Xinyu , Yan Ying , Wu Shujun , Cao Liming , Bian Hongwu , Yang Jinshui , Li Zhikang , Luo Xiaojin 
Published: March 2023

ABSTRACTAuxin is an important phytohormone in plants, and auxin signaling pathways in rice play key roles in regulating its growth, development, and productivity. To investigate how rice grain yield traits are regulated by auxin signaling pathways and to facilitate their application in rice improvement, we validated the functional relationships among regulatory genes such as OsIAA10, OsSK41, and OsARF21 that are involved in one of the auxin (OsIAA10) signaling pathways. We assessed the phenotypic effects of these genes on several grain yield traits across two environments using knockout and/or overexpression transgenic lines. Based on the results, we constructed a model that showed how grain yield traits were regulated by OsIAA10 and OsTIR1, OsAFB2, and OsSK41 and OsmiR393 in the OsSK41‐OsIAA10‐OsARF module and by OsARF21 in the transcriptional regulation of downstream auxin response genes in the OsSK41‐OsIAA10‐OsARF module. The population genomic analyses revealed rich genetic diversity and the presence of major functional alleles at most of these loci in rice populations. The strong differentiation of many major alleles between Xian/indica and Geng/japonica subspecies and/or among modern varieties and landraces suggested that they contributed to improved productivity during evolution and breeding. We identified several important aspects associated with the genetic and molecular bases of rice grain and yield traits that were regulated by auxin signaling pathways. We also suggested rice auxin response factor (OsARF) activators as candidate target genes for improving specific target traits by overexpression and/or editing subspecies‐specific alleles and by searching and pyramiding the ‘best’ gene allelic combinations at multiple regulatory genes in auxin signaling pathways in rice breeding programs.
Naïve Buying Diversification and Narrow Framing by Individual Investors
Authors: GATHERGOOD JOHN , HIRSHLEIFER DAVID , LEAKE DAVID , SAKAGUCHI HIROAKI , STEWART NEIL 
Published: March 2023

ABSTRACTWe provide the first tests to distinguish whether individual investors equally balance their overall portfolios (naïve portfolio diversification, NPD) or, in contrast, equally balance the values of same‐day purchases of multiple assets (naïve buying diversification, NBD). We find NBD in purchases of multiple stocks, and in mixed purchases of individual stocks and funds. In contrast, there is little evidence of NPD. Evidence suggests that NBD arises due to stock picking behavior and neglect of diversification. These findings suggest that behavioral finance theory should incorporate transaction, as well as portfolio, framing.
Self‐concealment, secrecy, and guilt
Authors: Davis Christopher G. 
Published: March 2023

AbstractObjectiveIndividuals with a tendency to conceal unflattering information about themselves are more likely to be preoccupied by their secrets and tend to report more negative affect. According to theory, this negative affect is due to self‐concealers' conflicting motivation to be authentic in their relationship but fear the negative consequences should they reveal their secrets, which promotes ill‐fated attempts to suppress. The purpose of the current study was to test a central component of this model.MethodsTwo studies of adults who were in a romantic relationship and keeping a secret from their partner (combined N = 635; 67.2% women; Mage = 39.6, SD = 11.9) were surveyed on four biweekly occasions. Multilevel mediation analyses were conducted to test whether preoccupation and suppression mediated the link between self‐concealing and negative affect and guilt.ResultsThe data support the hypotheses. Self‐concealers were more preoccupied with and prone to suppress their secret than those low on the trait, which, in turn, predicted greater negative affect and guilt.ConclusionThe findings suggest that self‐concealers' insecurities and fear of the relational consequences of disclosure set the stage for the debilitating cycle of suppression and preoccupation that leaves them feeling anxious and guilty.
Quality of low‐carbohydrate diets among Australian post‐partum women: Cross‐sectional analysis of a national population‐based cohort study
Authors: Lewandowski Sophie , Neale Elizabeth , D'Arcy Ellie , Hodge Allison M. , Schoenaker Danielle A. J. M. 
Published: March 2023

AbstractLow‐carbohydrate diets (LCDs) are popular among people attempting weight loss and recommended for pregnant women with gestational diabetes (GDM), but they may increase health risks if nutritionally inadequate. We aimed to describe the dietary intake of post‐partum women according to their relative carbohydrate intake, overall, and among women attempting weight loss or diagnosed with GDM in their recent pregnancy. This cross‐sectional population‐based cohort study included 2093 post‐partum women aged 25–36 years who participated in the Australian Longitudinal Study on Women's Health. Dietary intake was assessed using a validated food frequency questionnaire. Relative carbohydrate intake was determined using a previously developed LCD score. Data were weighted to account for oversampling of women from rural/remote areas. More than half of women (n[weighted] = 1362, 66.3%) were trying to lose weight, and 4.6% (n[weighted]=88) had GDM in their recent pregnancy. Women with the lowest relative carbohydrate intake (LCD score quartile 4) consumed 36.8% of total energy intake from carbohydrates, and had a lower intake of refined grains, whole grains, fruit and fruit juice, and a higher intake of red and processed meat, compared with women with the highest relative carbohydrate intake (quartile 1). Different food groups, both healthy and unhealthy, were restricted depending on whether women were attempting weight loss and had recent GDM. These findings may reflect a lack of knowledge among post‐partum women on carbohydrates and dietary guidelines. Health professionals may have an important role in providing advice and support for post‐partum women who wish to restrict their carbohydrate intake, to ensure optimal diet quality.
Intralesional steroid injections for inflammatory nail dystrophies in the pediatric population
Authors: Iorizzo Matilde , Gioia Di Chiacchio Nilton , Di Chiacchio Nilton , Grover Chander , Lipner Shari R. , Richert Bertrand , Piraccini Bianca Maria , Starace Michela , Tosti Antonella 
Published: March 2023

AbstractIntralesional injections of triamcinolone acetonide are widely used to successfully treat several inflammatory nail conditions. This procedure is well described in adults, but less frequently reported in children and teenagers, being largely considered too invasive and fear‐provoking for pediatric patients. Our report shows how this procedure is feasible and successful in children, even without a digital block. The step‐by‐step technique and tips to reduce pain should encourage clinicians to offer it as an alternative option to children with inflammatory nail disorders.
In utero acetaminophen exposure and child neurodevelopmental outcomes: Systematic review and meta‐analysis
Authors: Ricci Christina , Albanese Carmela Melina , Pablo Lesley A. , Li Jiaying , Fatima Maryam , Barrett Kathryn , Levis Brooke , Brown Hilary K. 
Published: March 2023

AbstractBackgroundAcetaminophen is a frequently used analgesic for pain and fever. There have been reports of adverse neurodevelopmental outcomes associated with in utero acetaminophen exposure. However, it is unclear whether this association is related directly to acetaminophen use, or the reasons for use.ObjectivesTo summarise the literature on the association between in utero acetaminophen exposure and child neurodevelopmental outcomes, and assess the extent to which the association is due to confounding by indication.Data SourcesOVID for Medline, Embase, and PsycINFO, and EBSCO for CINAHL, from inception to August 18, 2022.Study Selection and Data ExtractionWe searched for peer‐reviewed, English‐language studies on in utero acetaminophen exposure and child neurodevelopmental outcomes. Data were extracted using a standardised form created a priori, and quality was assessed using the Systematic Assessment of Quality in Observational Research.SynthesisWe generated pooled risk ratios (RR) for outcomes examined by ≥3 studies using random‐effects models; outcomes that could not be meta‐analysed were narratively summarised following Synthesis Without Meta‐Analysis guidelines.ResultsTwenty‐two studies including 23 cohorts were eligible (n = 367,775 total participants; median: 51.7% with acetaminophen exposure). Studies were primarily prospective cohort studies from Europe and the US, with attention deficit/hyperactivity disorder (ADHD) being the most common outcome. Quality assessments resulted in 13.6% of studies being classified as high, 59.1% as medium, 22.7% as low, and 4.5% as very low quality. In utero acetaminophen exposure was associated with an elevated risk of ADHD (unadjusted pooled RR 1.32, 95% confidence interval [CI] 1.20, 1.44; I2 = 47%, n = 7 studies), with little difference after adjusting for confounders, including indications for acetaminophen use (adjusted pooled RR 1.34, 95% CI 1.15, 1.55; I2 = 50%, n = 4 studies).ConclusionsConfounding by indication did not explain the association between in utero acetaminophen exposure and child ADHD. Further, high‐quality research is needed on this and other neurodevelopmental outcomes.
Pressure Vessel Design for High Temperature and Pressure Testing on Supercritical Carbon Dioxide
Authors: Putelli Lorenza , Ghidini Lorenzo , Solazzi Luigi , Iora Paolo Giulio 
Published: March 2023

Abstract
Supercritical carbon dioxide CO2 has been proposed as working fluid in various types of power plants, thanks to its potential higher efficiency and cost reduction with respect to well-known technology of steam cycles. However, the high operating temperature and pressure conditions pose significant concerns in terms of compatibility of supercritical CO2 with the high-temperature sections of the power block. Typically, to address this problem, experiments are proposed, where samples of different materials are kept in contact with the supercritical CO2 in a pressure vessel in order to test the material compatibility. This research deals with designing an innovative openable pressure vessel capable of withstanding the high temperatures (700 °C) and the pressures (100 bar) typical of those power plants. The results obtained by analytical calculation and the finite element method are consistent. The results obtained with both methods are generalizable and the methodology is applicable to any removable pressure vessel that must withstand 100 bar of pressure and a temperature of 700 °C, application field that is outside the ASME B&PV Code Section VIII division 1, standard usually used for the design of nonnuclear pressure vessels. It is important to emphasize that the use of a removable container allows to reduction of the costs of compatibility tests since otherwise, after each test, the pressure vessel would be disposed of. In addition, this allows a reduction of the environmental impact due to the fact that the new container will not be disposed of after each use.
Dynamic Response of 15 MW Floating Wind Turbine With Non-Redundant and Redundant Mooring Systems Under Extreme and Accidental Conditions
Authors: Niranjan Ramya , Ramisetti Srinivasa B. 
Published: March 2023

AbstractThis work focuses on examining the dynamic behavior of large floating offshore wind turbine (FOWT) exposed to extreme (wind and wave with 50-year return period) and accidental (mooring line breakage) loadings. The FOWT considered is 15 MW reference turbine supported on semi-submersible platform stationed using catenary moorings. As the mooring configuration greatly affects the response of FOWT, two different mooring configurations namely non-redundant (three-line) and redundant (six-line) systems were studied and compared. The coupled multi-body dynamic system was solved using Openfast. When simulating the mooring line failure, both the operating and extreme loading conditions were considered. Failure of one mooring was considered at a time. The response of the coupled system due to breakage of the mooring indicates high displacements in surge and sway directions in comparison to the intact system especially for the non-redundant mooring system. Furthermore, change in platform yaw angle and increased tension in the other intact mooring lines were observed. The findings from this study will be helpful in accidental limit state design and preventing failure of similar large FOWT systems. In addition, insights into using non-redundant and redundant mooring configurations for such large structures with respect to extreme and accidental loadings were also discussed.
Study of Performance of the Flexible (Al-Based) N-PVT-TEC Collectors in Different Configurations
Authors: Tiwari Gopal Nath , Singh Rohit Kumar , Sinha Akhoury Sudhir Kumar , Singh Amit Kumar 
Published: March 2023

AbstractThe opaque photovoltaic thermal (PVT) produces both thermal and electrical energy. In order to increase thermal energy, we have considered flexible (Al-based) photovoltaic (PV) module for the present study. Further, we have considered thermo-electric cooler (TEC) integrated with flexible PV module to enhance electrical power. As a result, an overall power can be increased in flexible PVT-TEC collector. A concept of series and parallel combination of flexible PVT-TEC collectors is proposed to optimize series (n) and parallel (m) combinations for a given number of N (=n × m) collectors for maximum overall exergy depending on thermal and electrical energies which have not been considered yet so far. Further, a new expression has also been developed for the heat removal factor and instantaneous thermal efficiency of the nth flexible PVT-TEC collector to investigate its effect on the nth flexible PVT-TEC collector performance. Numerical computations have been carried out for a given coldest climatic condition of Srinagar, India, and design parameters of Al-based PVT-TEC collectors using matlab R2021b. Based on numerical computations, the following conclusions have been drawn: (i) For case (a) (all flexible PVT-TEC collectors are connected in parallel), the daily overall exergy is 2.7 kW, which is 21.3% more than case (d). (All flexible PVT-TEC collectors are connected in series.) (ii) There is a drop of 20% in the mass flowrate factor due to the correction factor.