bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in postoperative knee function based on concomitant treatment of lateral meniscal injury in the setting of primary ACL reconstruction
Authors: Kaarre Janina , Herman Zachary J. , Persson Fabian , Wållgren Jonas Olsson , Alentorn-Geli Eduard , Senorski Eric Hamrin , Musahl Volker , Samuelsson Kristian 
Published: September 2023

Abstract
Background
Concomitant lateral meniscal (LM) injuries are common in acute anterior cruciate ligament (ACL) ruptures. However, the effect of addressing these injuries with various treatment methods during primary ACL reconstruction (ACLR) on patient-reported outcomes (PROs) is unknown. Therefore, the purpose of this study was to compare postoperative Knee injury and Osteoarthritis Outcome Score (KOOS) at 2-, 5-, and 10-years after isolated primary ACLR to primary ACLR with various treatment methods to address concomitant LM injury.

Methods
This study was based on data from the Swedish National Knee Ligament Registry. Patients ≥ 15 years with data on postoperative KOOS who underwent primary ACLR between the years 2005 and 2018 were included in this study. The study population was divided into five groups: 1) Isolated ACLR, 2) ACLR + LM repair, 3) ACLR + LM resection, 4) ACLR + LM injury left in situ, and 5) ACLR + LM repair + LM resection. Patients with concomitant medial meniscal or other surgically treated ligament injuries were excluded.

Results
Of 31,819 included patients, 24% had LM injury. After post hoc comparisons, significantly lower scores were found for the KOOS Symptoms subscale in ACLR + LM repair group compared to isolated ACLR (76.0 vs 78.3, p = 0.0097) and ACLR + LM injury left in situ groups (76.0 vs 78.3, p = 0.041) at 2-year follow-up. However, at 10-year follow-up, no differences were found between ACLR + LM repair and isolated ACLR, but ACLR + LM resection resulted in significantly lower KOOS Symptoms scores compared to isolated ACLR (80.4 vs 82.3, p = 0.041).

Conclusion
The results of this study suggest that LM injury during ACLR is associated with lower KOOS scores, particularly in the Symptoms subscale, at short- and long-term follow-up. However, this finding falls below minimal clinical important difference and therefore may not be clinically relevant.

Level of Evidence
III.
Interaction of estradiol and vitamin D with low skeletal muscle mass among middle-aged and elderly women
Authors: Zhang Jiaxing , Cheng Yalong , Chen Chen , Wang Qingan , Yang Chan , Qiu Jiangwei , Li Juan , Liu Xiaowei , Zhang Yuhong , Liu Lan , Zhao Yi 
Published: September 2023

Abstract
Background
Since the connection between muscle atrophy and vitamin D and estradiol status ambiguous, this study was thus conducted to determine whether low skeletal muscle mass (SMM) in middle-aged and elderly women was affected by estradiol and vitamin D levels together.

Methods
Baseline data from a sub-cohort of the China Northwest Natural Population Cohort: Ningxia Project (CNC-NX) were analyzed. Serum 25-hydroxyvitamin D (25(OH) D) and estradiol were measured by chemiluminescence immunoassay analyzer. Bivariate logistic regression and multiplicative interaction analyses were used to assess the impact of estradiol level and vitamin D status on low SMM, as well as the combined impact of estradiol and low vitamin D status on low SMM.

Results
A total of 287 (9.49%) participants had low SMM, which had lower levels of estradiol and vitamin D concentration than normal SMM group. While, after adjusting the confounding variables, these correlations were maintained in estradiol Q1, Q2, Q3 and vitamin D Q1. Furthermore, the significant combined effect of the highest quartile of estradiol concentrations and non-vitamin D deficiency, and interactions between vitamin D Q1 and estradiol Q2, vitamin D Q1 and estradiol Q3, vitamin D Q2 and estradiol Q1, vitamin D Q3 and estradiol Q3 on low SMM were stably reflected (P for interaction < 0.05).

Conclusions
Estradiol and vitamin D were interrelated with low SMM in middle-aged and elderly women. Combination of estradiol and vitamin D supplements should be encouraged for middle-aged and elderly women who are at risk of muscle atrophy or experiencing muscle atrophy.
High-throughput process development from gene cloning to protein production
Authors: Sun Manman , Gao Alex Xiong , Liu Xiuxia , Yang Yankun , Ledesma-Amaro Rodrigo , Bai Zhonghu 
Published: September 2023

AbstractIn the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.
Home-based transcranial alternating current stimulation (tACS) in Alzheimer’s disease: rationale and study design
Authors: Altomare Daniele , Benussi Alberto , Cantoni Valentina , Premi Enrico , Rivolta Jasmine , Cupidi Chiara , Martorana Alessandro , Santarnecchi Emiliano , Padovani Alessandro , Koch Giacomo , Borroni Barbara 
Published: September 2023

Abstract
Background
Gamma (γ) brain oscillations are dysregulated in Alzheimer’s disease (AD) and can be modulated using transcranial alternating stimulation (tACS). In the present paper, we describe the rationale and design of a study assessing safety, feasibility, clinical and biological efficacy, and predictors of outcome of a home-based intervention consisting of γ-tACS over the precuneus.

Methods
In a first phase, 60 AD patients will be randomized into two arms: ARM1, 8-week precuneus γ-tACS (frequency: 40 Hz, intensity: 2 mA, duration: 5 60-min sessions/week); and ARM2, 8-week sham tACS (same parameters as the real γ-tACS, with the current being discontinued 5 s after the beginning of the stimulation). In a second phase, all participants will receive 8-week γ-tACS (same parameters as the real γ-tACS in the first phase). The study outcomes will be collected at several timepoints throughout the study duration and include information on safety and feasibility, neuropsychological assessment, blood sampling, electroencephalography, transcranial magnetic stimulation neurotransmitter measures, and magnetic resonance imaging or amyloid positron emission tomography.

Results
We expect that this intervention is safe and feasible and results in the improvement of cognition, entrainment of gamma oscillations, increased functional connectivity, reduction of pathological burden, and increased cholinergic transmission.

Conclusions
If our expected results are achieved, home-based interventions using γ-tACS, either alone or in combination with other therapies, may become a reality for treating AD.

Trial registration
PNRR-POC-2022–12376021.
Sex differences in renal cell carcinoma: a single-cell analysis reveals exhausted CD8+ T-cells highly infiltrated in males
Authors: Ning Kang , Peng Yulu , Jiang Yue , Li Zhen , Luo Xin , Lin Lede , Deng Minhua , Wu Yi , Huang Tingxuan , Huang Yixin , Xie Ye , Yang Xiaofeng , Zhang Manhuai , Xiong Longbin , Zou Xiangpeng , Zhou Zhaohui , Zhou Fangjian , Dong Pei , Yu Chunping , Zhang Zhiling 
Published: September 2023

Abstract
Background
Although sex bias has been reported in the development and progression of renal cell carcinoma (RCC), the underlying mechanisms remain enigmatic. Here, we investigated the sex differences in the tumor microenvironment (TME) of RCC and explored a promising combination drug regimen to enhance the efficacy of immunotherapy.

Methods
Single-cell RNA sequencing (scRNA-seq) data from four published datasets were analyzed to investigate the sex differences in RCC patients, and tumor tissues were collected to validate the sex differences using multiplex immunofluorescence (MxIF) and flow cytometry (FCM). The function of the androgen–androgen receptor axis in sex differences was explored in vivo and in vitro experiments.

Results
Our analysis of scRNA-seq data from 220,156 cells, as well as MxIF and FCM assays, revealed that CD8+ T-cells infiltrated highly in the TME of male RCC, but were mostly in an exhausted and dysfunctional state. In vitro and in vivo experiments indicated that the dysfunction and exhaustion of CD8+ T-cells in male TME were induced by androgen. Clinically, higher serum androgen was significantly associated with a worse prognosis in male RCC patients receiving immunotherapy. Androgen receptor inhibitors could activate tumor-infiltrating CD8+ T-cells and enhance the efficacy of immunotherapy of RCC in vivo.

Conclusions
Our study delineated the difference in TME between male and female patients with RCC, and demonstrated that the androgen–androgen receptor axis plays an important role in immunosuppression in male RCC. Our findings suggest that androgen receptor inhibitors in combination with immunotherapy may be a promising treatment option for male RCC patients.
Quality evaluation of health information about breast cancer treatment found on WeChat public accounts
Authors: Yang Wenjuan , Li Bingyan , Liu Min , Tong Dongtong , Zou Yang , Li Xing , Xie Lunfang 
Published: September 2023

Abstract
Background
With growing cases of breast cancer, WeChat public account, an important information publishing platform of WeChat, has become a breast cancer treatment health information provider to a huge audience. It is essential for health information to possess high-level accuracy and reliability. This work evaluates the quality of health information on breast cancer treatment in WeChat public accounts (WPAs), to benefit the patients while making treatment decisions and provide WPA authors with suggestions on publishing high-quality treatment health information.

Methods
With “breast cancer” as keywords, searches were implemented on weixin.sogou.com and the WeChat app. The WPAs oriented to patients with breast cancer were selected, and the four latest articles of each WPA were included in a set to be evaluated with DISCERN.

Results
A total of 37 WPAs and 136 articles published by them were included. The accounts operated by individual users were 54%. The median of overall quality of 136 articles was 44 (interquartile range = 10.75) and ranked as “fair”, of which only 28 (21%) were of “good” or higher quality. Among these articles, 74 (54%) were related to medical treatments, and 13 of them mentioned clinical trials; 36 (27%) dealt with surgery. 101 (74.26%) omitted additional sources of information; 102 (75%) did not explicitly suggest shared decision-making. A significant difference was not found in the dimensions “reliability of the articles” and “specific details of information on treatment choices” between the distinct categories of account subjects and various treatment options (P > 0.05).

Conclusions
The quality of the articles on breast cancer treatment health information in WPAs was moderate. WPA producers should focus on improving the reliability of information and providing more details on treatment options, to assist patients in making optimal decisions during treatment.
What evidence exists on the ecological and physical effects of built structures in shallow, tropical coral reefs? A systematic map protocol
Authors: Paxton Avery B. , Swannack Todd M. , Piercy Candice D. , Altman Safra , Poussard Leanne , Puckett Brandon J. , Storlazzi Curt D. , Viehman T. Shay 
Published: September 2023

Abstract
Background
Shallow, tropical coral reefs face compounding threats from habitat degradation due to coastal development and pollution, impacts from storms and sea-level rise, and pulse disturbances like blast fishing, mining, dredging, and ship groundings that reduce coral reefs’ height and variability. One approach toward restoring coral reef structure from these threats is deploying built structures. Built structures range from engineered modules and repurposed materials to underwater sculptures and intentionally placed natural rocks. Restoration practitioners and coastal managers increasingly consider incorporating built structures, including nature-based solutions, into coral reef-related applications. Yet, synthesized evidence on the ecological and physical performance of built structure interventions across a variety of contexts (e.g., restoration, coastal protection, mitigation, tourism) is not readily available to guide decisions. To help inform management decisions, here we aim to document the global evidence base on the ecological and physical performance of built structures in shallow (≤ 30 m) tropical (35° N to 35° S latitude) coral ecosystems. The collated evidence base on use cases and associated ecological and physical outcomes of built structure interventions can help inform future consideration of built structures in reef restoration design, siting, and implementation.

Method
To discover evidence on the performance of built structures in coral reef-related applications, such as restoration, mitigation, and coastal protection, primary literature will be searched across indexing platforms, bibliographic databases, open discovery citation indexes, a web-based search engine, a novel literature discovery tool, and organizational websites. The geographic scope of the search is global, and there is no limitation to temporal scope. Primary literature will be screened first at the level of title and abstract and then at the full text level against defined eligibility criteria for the population, intervention, study type, and outcomes of interest. Metadata will be extracted from studies that pass both screening levels. The resulting data will be analyzed to determine the distribution and abundance of evidence. Results will be made publicly available and reported in a systematic map that includes a narrative description, identifies evidence clusters and gaps, and outlines future research directions on the use of built structures in coral reef-related applications.
Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts
Authors: Cuong Do Manh , Kim Dae Kyeong , Ediriweera Meran Keshawa , Park Jong-Eun , Moon Jeong Yong , Cho Somi Kim 
Published: September 2023

AbstractMelosira nummuloides is a marine diatom with potential use as food, fuel, and a dietary supplement. However, the efficacy of its extraction and drying techniques have not been explored. Here, M. nummuloides powders were prepared by two drying methods—hot-air drying (HAD) and freeze-drying (FD)—and extracted with hot water, ethanol, methanol, and chloroform:methanol (CM) at a ratio of 2:1 v/v. The antioxidant and anti-inflammatory activity of each extract was investigated. The CM extract had the greatest 2,2-diphenyl-1-picrylhydrazyl and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity among the solvent extracts, and a slight difference in antioxidant activity was observed across the various drying methods. Compared to other extracts, both the FD-CM and HAD-CM extracts showed stronger anti-inflammatory effects by inhibiting nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells. Furthermore, the FD-CM extract contained a wide range of lipophilic compounds. Notably, myristic acid (29.08 ± 0.45 mg/g dry weight powder extract (DW)), oleic acid (25.20 ± 0.92 mg/g DW), palmitoleic acid (10.77 ± 0.41 mg/g DW), eicosapentaenoic acid (12.53 ± 1.00 mg/g DW), neophytadiene (8.42 ± 0.51 mg/g DW), and α-linolenic acid (1.27 ± 0.005 mg/g DW) were among the prominent compounds identified. It is plausible to suggest that the abundance of these lipophilic compounds contributes to the remarkable antioxidative and anti-inflammatory potential exhibited by the FD-CM extract. Our results provide insights into the preferential drying methods and extraction solvents for producing M. nummuloides-based products with enhanced antioxidant and anti-inflammatory activity.
Effect of race on Gaze Cueing in adults with high and low autistic traits
Authors: Ricciardelli Paola , Pintori Noemi 
Published: September 2023

Abstract
Background
Observing the direction of gaze of another person leads to shifting of attention in the same direction (gaze-cueing effect – GCE), a social-cognitive ability known as joint or social attention. Racial attitudes can influence the magnitude of GCE since it has been shown that White people showing a strong race ingroup preference follow the gaze only of White, and not Black, faces. Individuals with high autistic traits have difficulties in social-cognitive abilities that can disrupt the learning of socially shared racial attitudes. Our aim was to investigate in White Italian adults whether individuals with higher autistic traits (measured by the Autism Spectrum Quotient) show reduced implicit racial bias (measured by the Implicit Association Test) and if this bias would lead to differences in the gaze cueing effect (GCE) triggered by gaze direction of faces of different races (measured by the Gaze Cueing Task).

Methods
In an online study, participants (N = 165; 132 females; Mean age = 22.9; SD = 4.76) filled in the Autism Spectrum Quotient (AQ) questionnaire, then performed a Gaze Cueing Task, followed and by an Implicit Association Test.

Results
Linear regression and linear mixed model analyses showed in the IAT task the presence of the same implicit ingroup bias for all participants, which was not predicted by the AQ score, while in the Gaze Cueing Task the GCE differed depending on the AQ score of the participants. Specifically, participants with low-medium, medium, and medium–high autistic traits (AQ = -1SD; AQ = mean; AQ =  + 1SD respectively) presented the GCE for both ingroup and outgroup cueing faces, whereas participants with high autistic traits (AQ =  + 2SD) only for ingroup faces.

Conclusions
In White Italian adults the presence of an implicit ingroup bias seems to influence the GCE, but it is not always true that the individuals showing an implicit ingroup bias do not orient their attention in the direction of gaze of the outgroup individuals. Instead, the GCE seems to be modulated by the level of autistic traits. That is, individuals with higher autistic traits seem to prioritize joint attention with only their ingroup members.
Photochemical and radiation transport model for extensive use (PROTEUS)
Authors: Nakamura Yuki , Terada Naoki , Koyama Shungo , Yoshida Tatsuya , Karyu Hiroki , Terada Kaori , Kuroda Takeshi , Kamada Arihiro , Murata Isao , Sakai Shotaro , Suzuki Yuhei , Kobayashi Mirai , Leblanc François 
Published: September 2023

AbstractWe introduce a new flexible one-dimensional photochemical model named Photochemical and RadiatiOn Transport model for Extensive USe (PROTEUS), which consists of a Python graphical user interface (GUI) program and Fortran 90 modules. PROTEUS is designed for adaptability to many planetary atmospheres, for flexibility to deal with thousands of or more chemical reactions with high efficiency, and for intuitive operation with GUI. Chemical reactions can be easily implemented into the Python GUI program in a simple string format, and users can intuitively select a planet and chemical reactions on GUI. Chemical reactions selected on GUI are automatically analyzed by string parsing functions in the Python GUI program, then applied to the Fortran 90 modules to simulate with the selected chemical reactions on a selected planet. We performed a benchmark test of PROTEUS to confirm its validity, by applying it to the Martian atmosphere and the Jovian ionosphere. PROTEUS can significantly save the time for those who need to develop a new photochemical model; users just need to write chemical reactions in the Python GUI program and just select them on GUI to run a new photochemical model.
Graphical Abstract
Optimal energy scheduling method for the North Cameroonian interconnected grid in response to load shedding
Authors: Ngoussandou Bello-Pierre , Nisso Nicodem , Kidmo Dieudonné Kaoga , Sreelatha E. , Jember Yosef Berhan , Das Sima , Kitmo  
Published: September 2023

AbstractRenewable energy sources like sun and wind are intermittent, hence a hybrid system incorporating them is necessary. However, certain systems are more cost-effective and efficient than others, and they are not only more expensive but also far more harmful to the environment. To make up for the energy shortage in unconnected remote regions and urban areas with linked networks, renewable energies offer an alternative. This effort is focused on eliminating both load shedding and the pollution caused by conventional power plants that burn fossil fuels. In order to improve the interconnected Northern Cameroon grid, researchers looked into the possibility of using the permanently accessible sun and wind at the Waibé-Lokoro-Kalfou location in Cameroon. For the hot and humid climate of Waibé-Lokoro, Cameroon, based on four distributed generations, four combinations were established in the scenario. The goal was to maximize the net present value while minimising the energy expense. Electricity costs were found to drop from USD 0.097/kWh to USD 0.085/kWh under the PV-Wind-Grid-Battery scenario, saving a total of USD 0.54 million in net present cost. The particle swarm optimization method (PSO), genetic algorithmic algorithm (GA), cuckoo search approaches (CSA), as well as whale optimization algorithm (WOA) were utilized to calculate power losses as well as system size allocation. PSO was the only algorithm to converge quickly. The level of distortion caused by harmonics is measured experimentally to verify that power grid connectivity regulations are being followed. The standards for the IEEE 33-bus as well as IEEE 69-bus tests provide more precise voltage profiles for use in loss evaluation.
Graphical Abstract
Contemporary challenges and AI solutions in port operations: applying Gale–Shapley algorithm to find best matches
Authors: Farzadmehr Mehran , Carlan Valentin , Vanelslander Thierry 
Published: September 2023

AbstractArtificial intelligence (AI) developments enable human capability to deliver the same outcome at a lower cost. This research performs a high-level matching between AI solutions and challenges within the port area by developing a novel academic approach. This way, the matching is carried out more structured than when one (manager, developer, challenge owner, etc.) fulfils it based on their opinion without following any structured approach. Therefore, the study defines first a comprehensive typology of port stakeholders' challenges, which can be solved via AI solutions. This typology presents challenges, including their main issues, widespread impact, and potential solutions. A state-of-the-art review of AI solutions that can address these challenges is carried out in parallel. Secondly, this review clearly distinguishes between AI solutions based on their technology and functionality. Thirdly, this research selects an appropriate AI solution for addressing each identified challenge in the port operation by upgrading the Gale–Shapley algorithm. Finally, it shows that the most critical presented AI solutions in this study use various machine learning (ML) techniques. Besides, concerning the AI solution's reusability feature and the result of high-level matching, this research shows that the implementation phase effort can be drastically reduced by using the recently developed matching algorithm.