bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rare and Intriguing Case of Wilson's Disease Initially Suspected of Systemic Lupus Erythematosus
Authors: Khodashahi Mandana , Mohajeri Najmeh , Alipour Moeid Reza , Khademi Reza , Roshan Nama Mohamadian , Aminzadeh Behzad , Joghatayi Muhammed 
Published: January 2025

ABSTRACTWhen systematic lupus erythematosus‐like lab results (e.g., positive anti‐double‐stranded DNA antibody, low complement component 3) are inconsistent with physical findings, such as the absence of arthritis or nephritis, clinicians should consider diagnoses such as Wilson's disease, especially in the presence of abnormal liver function and elevated international normalized ratio (INR).
Advance Care Planning Initiatives in Critical Care Centers in Japan: A Case Report
Authors: Kamimura Yuni , Tsubaki Michihiro , Hattori Jun , Oi Marina , Maruhashi Takaaki , Ota Haruyo , Oki Miho , Takanashi Kota , Takahashi Maika , Sakurai Yoko , Asari Yasushi 
Published: January 2025

ABSTRACTThis report examines the promotion of advance care planning (ACP) for patients admitted to critical care centers and discharged to home. Emergency transport experience allows patients and their families to realistically discuss her ACP.
Low‐Temperature Oxidation of Methane to Methanol on a Zeolitic Octahedral Metal Oxide
Authors: Guo Niu , Zhu Qianqian , Gou Ting , Hua Yihang , Zhang Lifeng , Li Yanshuo , Zhang Zhenxin 
Published: January 2025

AbstractDirect oxidation of methane to methanol utilizing molecular oxygen under mild conditions is an important yet challenging process due to the difficulty in activating of methane under such conditions. In this research, we report on zeolitic octahedral metal oxides based on cobalt vanadotungstates, which act as the catalysts for the oxidation of methane using molecular oxygen as the oxidant without co‐reductants at a low temperature of 90 °C even as low as 60 °C. This catalytic process results in the high‐yield production of methanol as the primary product. The micropores within the catalyst are critical for achieving high activity in the reaction, in which molecular oxygen is activated. The active species in the reaction are found to be hydroxyl‐based. Both VO5 with reduced V4+ and Co2+ are crucial for generating the active species for the reaction.
Synthesis and Redox Chemistry of Co3E4 (E=P, As) Clusters
Authors: Piesch Martin , Dinauer Sabrina B. , Riesinger Christoph , Balázs Gábor , Scheer Manfred 
Published: January 2025

AbstractThe synthesis of the cluster complexes [(Cp’’’Co)3(μ3,η2:η2:η2‐E3)(μ3‐E)] (E=P (3), As (4)) starting from the anionic triple‐decker complexes [K(18cr6)(dme)2][(Cp’’’Co)2(μ,η4:η4‐E4)] (E=P (1), As (2)) by electrophilic quenching with the Co dimer [(Cp’’’CoCl)2] is reported. Both complexes show a distinct redox chemistry, which was first investigated by cyclic voltammetry. Subsequently, the monoanions [K(L)(sol)n][(Cp’’’Co)3(μ3,η2:η2:η2‐E3)(μ3‐E)] (E=P, L=18cr6, sol=dme, n=2 (5), E=As, L=2,2,2‐crypt, n=0 (6)), the monocations [(Cp’’’Co)3(μ3,η2:η2:η2‐E3)(μ3‐E)][FAl] (E=P (7), As (8)) and the dications [(Cp’’’Co)3(μ3,η3:η3:η3‐E4)][TEF]2 (E=P (9), As (10)) could be realized experimentally and isolated in moderate to good yields. All compounds were characterized by single crystal X‐ray structure analysis, NMR and EPR spectroscopy, mass spectrometry and elemental analysis.
Synthesis of a Gemcitabine Prodrug and its Encapsulation into Polymeric Nanoparticles for Improved Therapeutic Efficacy
Authors: Kaliya Kajal , Bhardwaj Neha , Ruchika  , Saneja Ankit 
Published: January 2025

AbstractGemcitabine (GEM), a chemotherapeutic agent, is widely used to treat various neoplastic conditions, such as pancreatic, lung, breast, and ovarian cancer. However, its therapeutic effectiveness is often hindered by its short half‐life and susceptibility to enzymatic degradation. To address these limitations, in this research, five new conjugates of GEM were synthesized by conjugating its N‐4 amino group with five different acids [4‐decenoic acid (4Dec), lipoic acid (Lipo), lauric acid (Laur), 5‐benzyl N‐(tert‐butoxycarbonyl)‐ L‐glutamate (Glu), and decanoic acid (Dec)]. The anticancer potential of these conjugates was evaluated using CCK‐8 assay. Among the synthesized conjugates, 4Dec‐GEM demonstrated comparable cytotoxic activity to native GEM. The mechanistic insight of 4Dec‐GEM was investigated using annexin‐V FITC/propidium iodide staining, reactive oxygen species generation, and mitochondrial membrane potential loss assays. To further enhance its therapeutic efficacy, 4Dec‐GEM was encapsulated into poly(lactic‐co‐glycolic acid) (PLGA) nanoparticles using single‐emulsion method using high‐pressure homogenization. The developed nanoparticles were characterized by various techniques (TEM, FT‐IR, DSC, p‐XRD) and demonstrated successful entrapment of 4Dec‐GEM inside PLGA nanoparticles. Finally, the cytotoxicity of developed nanoparticles demonstrated improved anticancer activity as compared to native GEM in cancerous cell lines. Our study demonstrated that the combination of prodrug and nanoparticle approach can be a promising approach to augment the therapeutic efficacy of GEM.
A national estimate of mental disorders and mortality outcomes in cancer survivors
Authors: Mai Tran Thi Xuan , Chung Min Sung , Cha Chihwan , Park Boyoung 
Published: January 2025

AbstractBackgroundThis study evaluated the prevalence of various mental disorders and their influence on mortality outcomes in individuals with cancer.MethodsThe authors' institutional database included patients with cancer diagnosed between 2011 and 2015 who had mental disorders and death information up to 2021. Mental disorders included nonaffective psychotic disorders, affective psychotic disorders, anxiety‐related and stress‐related disorders, alcohol or drug misuse, and mood disorders without psychotic symptoms. The causes of death were classified as all‐cause, cancer‐related, or suicide. Individual matching was performed to randomly match cancer survivors with and without mental disorders according to age at cancer diagnosis, year of cancer diagnosis, sex, and cancer site. The association between mental disorders and mortality risk was assessed using a Cox proportional hazards model and competing‐risk analyses.ResultsAmong 637,491 cancer survivors (mean age, 58.9 years), there were 238,654 deaths from any cause and 2255 deaths from suicide. Incidence rates varied across disorders, with the highest rates observed for anxiety‐related and stress‐related disorders and mood disorders without psychotic symptoms. Mental disorders were associated with an increased risk of all‐cause and cancer‐related mortality. Adjusted hazard ratios (HRs) for nonaffective psychotic disorders, affective psychotic disorders, anxiety‐related and stress‐related disorders, alcohol and drug misuse, and mood disorders without psychotic symptoms were as follows: HR, 2.49 (95% confidence interval [CI], 2.22–2.80); HR, 2.38 (95% CI, 2.21–2.57); HR, 1.02 (95% CI, 1.01–1.04); HR, 2.13 (95% CI, 1.87–2.43); and HR, 1.27 (95% CI, 1.24–1.30), respectively, for all‐cause mortality. Suicide risk was higher in patients who had mental disorders, especially within the first 6 months after diagnosis.ConclusionsThe current findings underscore the impact of mental illness on mortality among cancer survivors in Korea, specifically highlighting the elevated rates of anxiety, stress, and mood disorders.
PARP‐1 Inhibition Increases Oxidative Stress in Ets‐1‐Expressing MDA‐MB‐231 Breast Cancer Cells
Authors: Hervieu Magalie , Legrand Arnaud J. , Floquet Emilie , Idziorek Thierry , Spriet Corentin , Monté Didier , Villeret Vincent , Aumercier Marc , Choul‐li Souhaila 
Published: January 2025

ABSTRACTBackgroundThe Ets‐1 transcription factor plays a primordial role in regulating the expression of numerous genes implicated in cancer progression. In a previous study, we revealed that poly(ADP‐ribose) polymerase‐1 (PARP‐1) inhibition by PJ‐34 results in Ets‐1 level increase in cells, which is related with cell death of Ets‐1‐expressing cancer cells.AimsThe mechanism of the antitumor effect of PARP‐1 inhibition was investigated in the Ets‐1‐expressing MDA‐MB‐231 breast cancer cells.Methods and ResultsWe tested the effects of four PARP inhibitors (PARPi) (PJ‐34, Veliparib, Olaparib, and Rucaparib). We first demonstrated that PARPi reduced cells growth through G2/M cell cycle arrest. Next, we evaluated PARP‐1 inhibition effect on oxidative DNA damage in Ets‐1‐overexpressing and Ets‐1‐non‐expressing breast cancer cells and we showed that PARPi led only Ets‐1‐overexpressing cells to accumulate it, which triggers the DNA damage response as revealed by the increase in the level of a panel of DNA damage‐related proteins. Importantly, we demonstrated that PARPi increased reactive oxygen species (ROS), only in Ets‐1‐overexpressing cells and this is accompanied by upregulation of p47phox expression, a subunit of the NAPDH oxidase (NOX).ConclusionThese preliminary findings correlate PARPi‐induced oxidative DNA damage/oxidative stress to Ets‐1 expression in breast cancer cells.
The Role of Social Initiatives in the Financial Success of Family Businesses
Authors: Orellana Alicia Ramírez , Escobar Silvia Giralt , González‐Tejero Cristina Blanco 
Published: January 2025

ABSTRACTSustainability and business success require ongoing analysis of variables that can impact the stability, efficiency, and evolution of family businesses. To this end, a study based on the environmental, social, and governmental (ESG) variables linked to financial performance was conducted on 93 publicly traded family businesses in the global retail and consumer markets sector in 2023, utilizing data from Thomson Reuters Eikon. Control variables, such as company size, number of employees, and indebtedness, were incorporated. The methodology employed involved a partial least squares structural equation modeling (PLS‐SEM) using SmartPLS. This analysis underscores the importance of social initiatives in enhancing financial outcomes for family businesses in the retail and consumer markets sector above the rest of the selected key variables. The originality of this research lies in its distinctive focus on the social dimension as the primary factor influencing the financial outcomes of family businesses. By highlighting the importance of social initiatives, the study provides an empirical basis that enables academics and business leaders to consider key variables in business development. This facilitates the design of strategies centered on these elements, contributing to the advancement of knowledge in the management of family businesses and their long‐term sustainability.
Energy‐Saving Electrochemical Hydrogen Production via Amorphous CoMoP/NF Bifunctional Catalyst Coupled with HMF Oxidation Reactions in Alkaline Electrolyte
Authors: Dong Ao , Guo Chengyang , Zhang Shuo , Song Yunjin , Deng Xiaoyan , Gao Hongtao 
Published: January 2025

AbstractTo improve water splitting efficiency and enhance energy utilization, it is crucial to develop catalysts with excellent activity, long‐term stability, and low cost. In this study, we synthesized a three‐dimensional nanostructured amorphous CoMoP/NF bifunctional catalyst for both the hydrogen evolution reaction (HER) and the 5‐hydroxymethylfurfural oxidation reaction (HMFOR), using a sacrificial template method. Benefiting from element doping regulation and morphology control, CoMoP/NF exhibited outstanding catalytic activity. Notably, in an electrolytic water system coupled with HER and HMFOR, a cell voltage of only 1.5 V was required at a current density of 20 mA cm−2, approximately 189 mV lower than that of a traditional water electrolysis system. This study presents a novel energy‐saving method for hydrogen production through the HER/HMFOR electrolysis system.
Thyroglobulin mRNA ectopic expression was an excellent marker to detect lymph node metastasis for patients with papillary thyroid cancer
Authors: Du Wen‐Hua , Chen Ying‐Chao , Jin Ning , Zhang Yuan‐yuan , Ji Bao‐Lan , Wu Jing , Wu Feng‐Yao , Zhao Shuang‐Xia , Han Bing , Song Huai‐Dong , Dong Mei 
Published: January 2025

AbstractObjectiveTo investigate whether the mRNA expression of thyroglobulin (TG) and TSH receptor (TSHR) in lymph node could be used to diagnose lymph node metastasis in patients with papillary thyroid cancer (PTC).Subjects and methodsAround 156 paraffin samples of lymph nodes from 89 patients with PTC after surgery were collected, and the expressions of TG and TSHR mRNA were detected by nested qPCR.ResultsCompared with the results of confirmed histopathology, 86 out of 87 tissues of lymph nodes metastasis ectopically expressed TG mRNA, while 66 out of 69 tissues of non‐metastasis lymph nodes did not express the TG mRNA. The specificity and sensitivity of TG mRNA measurement for detecting the lymph node metastasis were 95.65% and 98.85%, as effective as the first postoperative histopathology. However, the specificity and sensitivity of TSHR mRNA measurement were 92.75% and 85.06%, respectively. The accuracy of TG, TSHR mRNA measurement and the first postoperative histopathology were 97.44%, 88.46% and 95.51%, with the positive predictive rate (PPR) 96.63%, 93.67%, and 98.78%, respectively, negative predictive rate (NPR) 98.51%, 83.12% and 91.89%, respectively, as well as Youden's index 0.95, 0.78 and 0.92.ConclusionsTG mRNA ectopic expression in the lymph node might be an excellent marker to diagnose the lymph node metastasis for patients with PTCs.
Identification of CENPM as a key gene driving adrenocortical carcinoma metastasis via physical interaction with immune checkpoint ligand FGL1
Authors: Zou Cunru , Zhang Yu , Liu Chengyue , Li Yaxin , Lin Congjie , Chen Hao , Hou Jiangping , Gao Guojun , Liu Zheng , Yan Qiupeng , Su Wenxia 
Published: January 2025

AbstractBackgroundDistant metastasis occurs in the majority of adrenocortical carcinoma (ACC), leading to an extremely poor prognosis. However, the key genes driving ACC metastasis remain unclear.MethodsWeighted gene co‐expression network analysis (WGCNA) and functional enrichment analysis were conducted to identify ACC metastasis‐related genes. Data from RNA‐seq and microarray were analyzed to reveal correlations of the CENPM gene with cancer, metastasis, and survival in ACC. Immunohistochemistry was used to assess CENPM protein expression. The impact of CENPM on metastasis behaviour was verified in ACC (H295R and SW‐13) cells and xenograft NPG mice. DIA quantitative proteomics analysis, western blot, immunofluorescence, and co‐immunoprecipitation assay were performed to identify the downstream target of CENPM.ResultsAmong the 12 035 analyzed genes, 363 genes were related to ACC metastasis and CENPM was identified as the hub gene. CENPM was upregulated in ACC samples and associated with metastasis and poor prognosis. Knockdown of CENPM inhibited proliferation, invasion, and migration of ACC cells and suppressed liver metastasis in xenograft NPG mice. Collagen‐containing extracellular matrix signalling was primarily downregulated when CENPM was knocked down. FGL1, important components of ECM signalling and immune checkpoint ligand of LAG3, were downregulated following CENPM silence, overexpressed in human advanced ACC samples, and colocalized with CENPM. Physical interaction between CENPM and FGL1 was identified. Overexpression of FGL1 rescued migration and invasion of CENPM knockdown ACC cells.ConclusionsCENPM is a key gene in driving ACC metastasis. CENPM promotes ACC metastasis through physical interaction with the immune checkpoint ligand FGL1. CENPM can be used as a new prognostic biomarker and therapeutic target for metastatic ACC.Highlights
CENPM is the key gene that drives ACC metastasis, and a robust biomarker for ACC prognosis.
Silencing CENPM impedes ACC metastasis in vitro and in vivo by physical interaction with immune checkpoint ligand FGL1.
FGL1 is overexpressed in ACC and promotes ACC metastasis.
Cuckoo Hosts Fine‐Tune Their Egg Rejection After Experiencing a Parasitism Event
Authors: Li Bin , Wang Longwu , Liu Jianping , Liang Wei 
Published: January 2025

ABSTRACTRecognising and rejecting parasitic eggs is one of the most common anti‐parasitism strategies used by host birds. However, the egg rejection of some hosts exhibits behavioural plasticity. To investigate whether the egg rejection behaviour of host birds changes after encountering a parasitism event, we conducted egg rejection experiments on the locally most common host of the common cuckoo (Cuculus canorus), the grey bushchat (Saxicola ferreus) in Yunnan, China. When a single pure white egg of the white‐rumped munia (Lonchura striata) or a blue model egg was individually added to the nest of the grey bushchat, the rejection rate for the white‐rumped munia eggs was as high as 93.3%, whereas the rejection rate for the blue model egg was minimal (5.56%). However, when the grey bushchat rejected the munia egg and a blue model egg was subsequently added to its nest, the rejection rate for the blue model egg was significantly higher, reaching 54.5%. When recognised, the presence of a non‐mimetic foreign egg in the nest may then act as a cue indicating high parasitism risk for the host. Consequently, the bird may intensify its inspection of nest eggs, leading to increased rejection of the previously accepted blue model eggs. Our results are consistent with the optimal acceptance threshold hypothesis, suggesting that as the parasitism risk increases, the grey bushchat adjusts its acceptance threshold for foreign eggs to become more stringent.
Persistence of Reptile DNA in a Terrestrial Substrate: A Case Study Using the Eastern Indigo Snake
Authors: Samuels Leah R. N. , Chandler Houston C. , Hoffman Michelle , Kronenberger John A. , Elmore Michele , Aldredge Robert , Stegenga Benjamin S. , Bogan James E. , Davis Mark A. , Hertz Stephanie , Schwartz Michael K. , Wilcox Taylor 
Published: January 2025

ABSTRACTEnvironmental DNA (eDNA) analysis of terrestrial substrates, such as soil and sand, is a rapid and potentially cost‐effective way to monitor rare wildlife species. A promising use‐case in the southeastern United States is provided by the eastern indigo snake (Drymarchon couperi), for which accurate monitoring has been challenging due to large home ranges and low‐density populations. However, knowledge gaps regarding eDNA deposition and persistence in this system currently limit our ability to apply eDNA sampling effectively at the landscape scale. To overcome some of these gaps, we used an optimized soil and sand eDNA extraction protocol and species‐specific qPCR assay to conduct a full factorial experiment of eastern indigo snake DNA detection in sand as a function of the duration of snake presence and time since snake removal. We then used these data and a generalized linear mixed model to predict detection probability. Of the 224 total experimental samples, 68 (30.4%) tested positive for eastern indigo snake eDNA. Our model predicted that, with long periods in the enclosure and sampling soon after snake removal, eastern indigo snake eDNA is detectable 68.7% of the time. Eastern indigo snake DNA was detectable in as little as 100 s of snake presence in the enclosure (Pr = 21.1%) and for as long as 10 days after snake presence (Pr = 27.7%). These results suggest that DNA sampling in terrestrial systems may be an effective tool for increasing the temporal window of rare snake detection and a useful complement to existing sampling methods for eastern indigo snakes.
Survival of Human Bone Marrow Plasma Cells In Vitro Depends on the Support of the Stromal Cells, PI3K, and Canonical NF‐kappaB Signaling
Authors: Uyar‐Aydin Zehra , Kadler Shirin , Lauster Roland , Bartfeld Sina , Rosowski Mark 
Published: January 2025

ABSTRACTContrary to short‐lived plasma cells, which survive only 3–5 days, long‐lived plasma cells (LLPCs) contribute to the humoral memory of the body and thus also to many antibody‐related diseases. The ability of plasma cells to persist over months, years, and even a lifetime has been demonstrated in vivo. Yet, the in vitro culture of human primary bone marrow‐derived plasma cells has been limited to a few days. Here, we establish culture conditions for human primary bone marrow‐derived plasma cells for 21 days. Plasma cells and stromal cells are isolated from human bone marrow and cultured in 2D or a 3D ceramic scaffold. The plasma cells’ survival and antibody secretion depend on direct contact with stromal cells. The culture promotes CD19‐negative PCs. Inhibition of the PI3K or NF‐kappaB pathways using chemical inhibitors reduced the survival of the plasma cells. These results underline the supportive role of the stromal cells for the survival of the LLPC and confirm mechanisms that were identified in mouse LLPCs also for human LLPCs. The culture described here will promote further studies to deepen our understanding of the human LLPC.
An Explainable ADASYN‐Based Focal Loss Approach for Credit Assessment
Authors: Ali Shahee Shaukat , Patel Rujavi 
Published: January 2025

ABSTRACTThe integration of deep learning techniques with financial technology (fintech) has revolutionized the credit risk analysis, a critical component of financial risk management. A pervasive challenge in credit risk assessment lies in the skewed distribution of data, hindering accurate predictions, particularly for minority class instances. In available literature, various solutions have been proposed to address class imbalance, albeit with limitations. Focal loss is one of the well‐known loss functions proposed for handling class imbalance by running the hyperparameter . However, imbalance still remains in terms of number of hard‐to‐learn observations between the classes. In this paper, we have proposed integration of ADASYN with focal loss to mitigate class imbalance and enhance credit scoring accuracy. ADASYN systematically generates synthetic data based on hard‐to‐learn examples to counter skewed distributions, while focal loss prioritizes the training of challenging examples, fostering a more balanced model performance. This approach has been rigorously tested using real‐world imbalanced datasets and credit assessment data, and the outcomes have been compared against a range of sample technique and loss function combinations. The results clearly show that our suggested strategy is better than other approaches. Although improving the accuracy of credit risk analysis is critical, model interpretability is just as important for enabling financial analysts to make wise choices. In order to solve this, we have measured the global and local contributions of each feature using SHAP (Shapley additive explanation). According to global interpretability, the top 4 parameters influencing credit risk assessment are checking account status, loan purpose, borrower age, credit history, and interest rate/installment rate. Moreover, local interpretability analysis reveals quantitative and direction differences in feature contributions. These revelations not only broaden our knowledge of credit assessment services but also highlight how important a role they could play in attracting new clients and generating income. This paper also highlights how the suggested approach may be scaled to other imbalanced real‐world datasets, demonstrating how it can improve model performance in terms of AUC, G‐mean, and F‐measure.