bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving split reporters of protein-protein interactions through orthology-based protein engineering
Authors: Rakotoarison Louise-Marie , Tebo Alison G. , Böken Dorothea , Gautier Arnaud 
Published: September 2023

SummaryProtein-protein interactions (PPI) can be detected through selective complementation of split fluorescent reporters made of two complementary fragments that reassemble into a functional fluorescent reporter when in close proximity. We previously introduced splitFAST, a chemogenetic PPI reporter with rapid and reversible complementation. Here, we present the engineering of RspA-splitFAST, an improved reporter displaying higher brightness, lower self-complementation and higher dynamic range for optimal monitoring of PPI using an original protein engineering strategy that exploits proteins with orthology relationships. Our study allowed the identification of a system with improved properties and enabled a better understanding of the molecular features controlling the complementation properties. Because of the rapidity and reversibility of its complementation, its low self-complementation, high dynamic range, and improved brightness, RspA-splitFAST is well suited to study PPI with high spatial and temporal resolution, opening great prospects to decipher the role of PPI in various biological contexts.
Outer mitochondrial membrane E3 Ub ligase MARCH5 controls mitochondrial steps in peroxisome biogenesis
Authors: Verhoeven Nicolas , Oshima Yumiko , Cartier Etienne , Neutzner Albert , Boyman Liron , Karbowski Mariusz 
Published: September 2023

AbstractPeroxisomede novobiogenesis requires yet unidentified mitochondrial proteins. We report that the outer mitochondrial membrane (OMM)-associated E3 Ub ligase MARCH5 is vital for generating mitochondria-derived pre-peroxisomes. MARCH5 knockout results in accumulation of immature peroxisomes and lower expression of various peroxisomal proteins. Upon fatty acid-induced peroxisomal biogenesis, MARCH5 redistributes to newly formed peroxisomes; the peroxisomal biogenesis under these conditions is inhibited in MARCH5 knockout cells. MARCH5 activity-deficient mutants are stalled on peroxisomes and induce accumulation of peroxisomes containing high levels of the OMM protein Tom20 (mitochondria-derived pre-peroxisomes). Furthermore, depletion of peroxisome biogenesis factor Pex14 leads to the formation of MARCH5- and Tom20-positive peroxisomes, while no peroxisomes are detected in Pex14/MARCH5 dko cells. Reexpression of WT, but not MARCH5 mutants, restores Tom20-positive pre-peroxisomes in Pex14/MARCH5 dko cells. Thus, MARCH5 acts upstream of Pex14 in mitochondrial steps of peroxisome biogenesis. Our data validate the hybrid, mitochondria-dependent model of peroxisome biogenesis and reveal that MARCH5 is an essential mitochondrial protein in this process.SummaryThe authors found that mitochondrial E3 Ub ligase MARCH5 controls the formation of mitochondria-derived pre-peroxisomes. The data support the hybrid, mitochondria-dependent model of peroxisome biogenesis and reveal that MARCH5 is an essential mitochondrial protein in this process.
Validation of Digital Pathology Platform for Metabolic-Associated Steatohepatitis for Clinical Trials
Authors: Pulaski Hanna , Mehta Shraddha S. , Manigat Laryssa C. , Kaufman Stephanie , Hou Hypatia , Nalbantoglu ILKe , Zhang Xuchen , Curl Emily , Taliano Ross , Kim Tae Hun , Torbenson Michael , Glickman Jonathan N , Resnick Murray B , Patel Neel , Taylor Cristin E. , Bedossa Pierre , Montalto Michael C , Beck Andrew H , Wack Katy E 
Published: September 2023

AbstractAimsDetermine if pathologic assessment of disease activity in steatohepatitis, performed using Whole Slide Images (WSIs) on the AISight Clinical Trials platform, yields results that are comparable to those obtained from the analysis performed using glass slides.Methods and ResultsThe accuracy of scoring for steatohepatitis (NAS ≥4 with ≥1 for each feature and absence of atypical features suggestive of other liver disease) performed on the WSI viewing platform was evaluated against scoring conducted on glass slides. Both methods were assessed for overall percent agreement (OPA) with a consensus ‘ground truth’ (GT) score, defined as the median score of a panel of 3 expert pathologists on glass slides. Each case was also read by 3 different pathologists, once on glass and once using WSIs with a minimum 2-week washout period between glass and WSI reads. It was demonstrated that the average OPA across 3 pathologists of WSI scoring with GT was non-inferior to the average OPA of glass scoring with GT (non-inferiority margin of -0.05, difference of -0.001, 95% CI of (−0.027,0.026), and p<0.0001). For each pathologist, there was a similar average OPA of WSI and glass reads with glass GT (pathologist A 0.843 and 0.849, pathologist B 0.633 and 0.605 and pathologist C 0.755 and 0.780), with intra-reader, inter-modality agreements per histologic feature being greater than published intra-reader agreements.ConclusionAccuracy of digital reads for steatohepatitis using WSIs is equivalent to glass reads in the context of a clinical trial for scoring using the Clinical Research Network scoring system.
Partnering for impact: unveiling the dynamics of collaboration between social enterprises and impact investors in emerging economies
Authors: Emmanuel Jeremiah Arigu , Wijewardena Chanaka , Rammal Hussain Gulzar , Khakhar Priyan Pravin 
Published: September 2023

Purpose
This study empirically aims to examine the collaboration between social enterprises (SEs) and impact investors (IIs), which are organisations with similar interests but with distinct logics, and in high demand in emerging economies with complex problems. Despite the significant economic contributions of these organisations, there have been limited studies examining how they collaborate in different contexts, including theoretical insights explaining how they gain partner fit from resource synergy.

Design/methodology/approach
Mainstream businesses use the compatibility and complementarity concepts to examine buyer–supplier strategic alliances. Using similar concepts in the context of hybrid organisations, the authors interviewed six pairs of SEs and IIs with dyadic relations in Nigeria, aiming to deeply understand how they align dissimilar logics in pursuing common goals in emerging economies.

Findings
The authors’ findings revealed how compatibility criteria from the institutional logics perspective and complementarity from social exchange theory guide collaboration between SEs and IIs in an emerging economy. Using these theories provides new insights that distinguish SEs and IIs collaboration from conventional theories on the internationalisation of businesses, which remained insufficient for understanding the cross-border operations of SEs.

Practical implications
The study holds practical implications for organisations, regardless of their size, international investors, governments, organisations and individuals desiring to pursue sustainable business agendas in emerging economies with huge impact opportunities and the process involved.

Originality/value
The outcomes of this study extend knowledge of the theoretical lens examining collaborative entrepreneurship from the perspective of hybrid organisations. It also challenged existing knowledge on collaboration between SEs and IIs, often characterised by potential tensions due to the dissimilarity of institutional logics of actors.
How does compulsory citizenship behavior disturb police employees' psychological health? The roles of work–family conflict and putting family first
Authors: Chaudhary Arooba , Cheema Amna Umer , Sheikh Labiba , Islam Talat 
Published: September 2023

PurposeThis study investigates how compulsory citizenship behavior (CCB) restricts police employees from fulfilling their family responsibilities [i.e. work–family conflict (WFC)] and affects their psychological health. The authors also examined putting family first (PFF) as a conditional variable on the association between CCB and WFC.Design/methodology/approachThis quantitative study collected data from 341 police employees on convenience basis. Further, the authors tackled the issue of common method bias (CMB) by collecting data in two waves.FindingsThe data were analyzed through structural equation modeling (SEM), and the result revealed that WFC mediates the association between CCB and police employees' psychological health. In addition, the authors noted that individuals high in PFF were less likely to experience WFC in the presence of CCB.Originality/valueThis study contributes to the scant literature on police employees' psychological health. Specifically, this study is the first to investigate the mediating role of WFC between CCB and psychological health with the boundary condition of PFF.
Tourism gentrification and neighbourhood transformation
Authors: Lo Jamie Siu Kam , McKercher Bob 
Published: September 2023

Purpose
This study aims to examine the process of tourism gentrification from China tourists, a major source market, in two neighbourhoods in Hong Kong: one that has been long exposed to tourism and one that is just being discovered by tourists. Through a series of in-depth interviews with local residents and community leaders, complemented by non-participant observation, the paper tracks the commercial, social and cultural changes that have occurred or are occurring in these two places.

Design/methodology/approach
This study evaluates the process of place change through tourism gentrification in two neighbourhoods in Hong Kong that are at different stages of the process. An ontological approach is adopted using an interpretivist paradigm involving in-depth interviews and on-site observations, supplemented by secondary data. These data were complemented by non-participant observation.

Findings
Both the places have been transformed due to the influx of mainland Chinese tourists. Some impacts felt by both communities have much in common, such as congestion and shop dislocation, while others are more location specific. Even though the degree of tourism gentrification is different, locals from both locations tend to hold negative perceptions towards tourists, although of different strengths. All have also noted the traditional social cohesion is not as strong as before.

Originality/value
The paper argues that tourism gentrification exists on a continuum as neighbourhoods move seemingly inextricably from local-centric nodes to tourist-centric nodes. Apart from enlightening the theory of tourism gentrification, it provides insights to the local leaders on proper tourism development by balancing the social and economic benefits.
Use of emerging social media platforms in reshaping the UAE Islamic banks’ promotional strategies
Authors: Muhammad Abid Mahmood , Basha Mohamed Bilal , AlHafidh Gail 
Published: September 2023

Purpose
This paper aims to investigate customer attitude towards the use of emerging social media platforms (SMPs) for promotional activities by United Arab Emirates (UAE)-based Islamic banks, particularly, in the post-COVID-19 era. The key drivers of this research include analysing, anticipating and providing recommendations to reinvigorate the marketing and promotional strategies of the UAE Islamic banks to spark renewed customer interest.

Design/methodology/approach
This study is anticipatory and descriptive in nature. Primary data is used to understand customer perception towards the use of emerging social media marketing tools by the UAE-based Islamic banks. Reliability, factor analysis and multiple regression analysis are applied to understand customer attitude. While focusing on the current COVID-19 scenario, the need for innovative structure is envisaged to meet the post-COVID-19 needs.

Findings
The findings of this research highlight the significance of emerging SMPs such as WhatsApp, TikTok, Pinterest, Viber, Snapchat and their application as promotional tools to inspire the purchase intention of customers in this virtual age. The results of the study reveal these emerging SMPs are predicted to be used as the preferred promotional tools for Islamic banks.

Research limitations/implications
This paper is limited to the UAE Islamic banks and to a specified set of SMPs as promotional tools. Nevertheless, its findings have important implications that can be extended and validated through studying the post-COVID-19 customer attitude towards other innovative promotional tools used by commercial banks in general and Islamic banks in particular, in the GCC and Middle East and North Africa (MENA) regions.

Originality/value
There is currently limited available research on the innovative social media marketing techniques and promotional strategies. This study is a novel attempt to examine the adoption of the emerging SMPs as promotional tools by the UAE Islamic banks. This paper extends value to the existing studies on the impact of the pandemic on the promotional activities of the UAE Islamic banking industry. Nonetheless, while regionally specific, it is valuable in its potential application to the Islamic banking sector in the entire GCC and MENA region in the post-COVID-19 era.
Fostering innovation capability and sustainable innovation in halal industry: the role of halal entrepreneurs’ success
Authors: Salaheldeen Mohamed , Battour Mohamed 
Published: September 2023

Purpose
This study aims to conceptualize and investigate the relationships between halal entrepreneurial success, innovation capability and sustainable innovation in the halal industry.

Design/methodology/approach
Both integrative and generative approaches are combined to move forward from the literature to a theoretical contribution. The paper presents a model that relates halal entrepreneurial success to innovation capability and sustainable innovation. A purposive sample of 340 Malaysian halal entrepreneurs is used to test the conceptualized model. The partial least square technique was then used to assess the structural model.

Findings
The results disclosed that halal entrepreneurial success is positively related to both innovation capability and sustainable innovation. Also, the innovation capability mediates the relationship between halal entrepreneurial success and sustainable innovation.

Originality/value
This research conceptualized and examined a novel model that assesses the relationships between halal entrepreneurial success, innovation capability and sustainable innovation in the halal industry.
Proportional appropriation systems and financial statement quality in municipally owned entities: empirical evidence from Italy
Authors: Capalbo Francesco , Galati Luca , Lupi Claudio , Smarra Margherita 
Published: September 2023

PurposeThis paper aims to examine how proportional appropriation systems affect the quality of financial reporting in entities controlled by local governments.Design/methodology/approachThe authors examine this issue using the setting of Italian municipally owned entities (MOEs) following the implementation of a new accounting regulation that limits the spending power of the participating municipality when the owned entity reports losses. The authors apply Benford's law on net income figures using the Chi-square and Z-tests on the adjusted version of the Mean Absolute Deviation (MAD) criterion to spot any sign of low data quality. The sample, which consists of 2,120 MOEs, covers the years 2010–2019 and is evenly divided into the periods pre- and post-policy introduction.FindingsWidespread data anomalies were detected following the introduction of the new regulation for MOEs controlled by local governments. Evidence is stronger for entities owned entirely by municipalities. The results suggest that the extent of data manipulation grows as the municipality's ownership stake increases, consistent with the hypothesis that a decrease in spending power through the appropriation of financial resources affects earnings management practices in municipally controlled entities.Practical implicationsThis paper sheds light on government-based accounting policies by documenting evidence of somewhat inefficient responses by those responsible for the preparation of financial statements on behalf of municipally owned entities, and, accordingly, insights are provided to help review these policies so as to forestall even indirectly detrimental repercussions on public services.Originality/valueThis paper extends prior research in public-sector earnings management by being the first to test whether MOEs manipulate their earnings as a consequence of participating municipalities' reduced spending capability. Understanding factors influencing earnings management practices driven by governments, other than political incentives, is still an open issue.
Understanding the role of customer incivility and supervisor monitoring in the relationship between customer orientation and frontline employees' emotional exhaustion
Authors: Xie Fang , Zhang Xufan , Ye Jing , Zhou Lulu , Zhang Wenjian , Tian Feng 
Published: September 2023

PurposeBased on the resource conservation theory, this research paper aims to evaluate the positive impact of customer orientation on frontline employees' emotional exhaustion and the moderating effects of customer incivility and supervisor monitoring.Design/methodology/approachTwo-wave data from 484 frontline employees in power supply business halls were analyzed. This study used AMOS 23.0, SPSS22.0 and PROCESS macro for data statistics and analysis.FindingsOur empirical research demonstrates that customer orientation has a significant positive impact on frontline employees' emotional exhaustion. At the same time, supervisor monitoring moderates the relationship between customer orientation and emotional exhaustion. The higher the interactional or observational monitoring, the stronger customer orientation's effect on frontline employees' emotional exhaustion. Moreover, a three-way interaction model exists between customer orientation, customer incivility and supervisor monitoring.Practical implicationsThis study yields practical implications for helping the frontline employees of service-oriented organizations alleviate multiple interpersonal workplace pressures.Originality/valueBased on resource conservation theory, this paper used a novel approach to focus on customer orientation, customer incivility and supervisor monitoring as interpersonal stressors.
Predicting customer deposits with machine learning algorithms: evidence from Tunisia
Authors: Gafrej Oussama 
Published: September 2023

PurposeThis paper aims to evaluate the performance of the multiple linear regression (MLR) using a fixed-effects model (FE) and artificial neural network (ANN) models to predict the level of customer deposits on a sample of Tunisian commercial banks.Design/methodology/approachTraining and testing datasets are developed to evaluate the level of customer deposits of 15 Tunisian commercial banks over the 2002–2021 period. This study uses two predictive modeling techniques: the MLR using a FE model and ANN. In addition, it uses the mean absolute error (MAE), R-squared and mean square error (MSE) as performance metrics.FindingsThe results prove that both methods have a high ability in predicting customer deposits of 15 Tunisian banks. However, the ANN method has a slightly higher performance compared to the MLR method by considering the MAE, R-squared and MSE.Practical implicationsThe findings of this paper will be very significant for banks to use additional management support to forecast the level of their customers' deposits. It will be also beneficial for investors to have knowledge about the capacity of banks to attract deposits.Originality/valueThis paper contributes to the existing literature on the application of machine learning in the banking industry. To the author's knowledge, this is the first study that predicts the level of customer deposits using banking specific and macroeconomic variables.
Unveiling consumer behavior in marketing: a meta-analytic structural equation modeling (Meta-SEM) of the model of goal-directed behavior (MGB)
Authors: Vishwakarma Pankaj , Mohapatra Malaya Ranjan 
Published: September 2023

PurposeUnderstanding consumer behavior across various contexts within marketing has long been the focus of studies. Although many models are used in explaining consumers' behavior, one of these is the Model of Goal-Directed Behavior (MGB), which is becoming prominent in explaining consumers' behavior in marketing. Given its popularity, prior research on MGB has shown inconsistent outcomes regarding the casual association of MGB variables. To overcome this, the authors have adopted a meta-analytic review of the marketing studies grounded on MGB theory in examining the consumers' behavior.Design/methodology/approachThe study reviewed and analyzed 611 correlations from 27 studies with 31 samples (combined sample size of 9588) using a meta-analytic structural equation modeling (MASEM) technique.FindingsThe outcomes of MASEM confirm the significance of all the proposed relationships in the MGB model. However, the attitude has shown a strong influence on desire formation among all the proposed MGB relationships. Further, past buying experience and positive anticipated emotions strongly affect desire in developed nations compared to developing nations.Research limitations/implicationsThe current work has considered the possibility of various recommended moderators (e.g. culture, crisis situation, sample size, method of data collection, etc.); however, the study lacks to consider the dimension of gender dominance in it. Hence, future researchers should keep it in mind while conducting similar studies. Future scholars can also perform a comparative study on MGB across the domains and subdomains to know more insights.Originality/valueThe current work offers a better understanding of MGB application in marketing. As this work is one of the first meta-analyses on MGB application in marketing that also considers the effect of various moderators, it thus adds knowledge to the literature on MGB in marketing. It will also help the future researchers to understand MGB as a framework and its application in marketing.
Exploring Arab researchers' research data sharing and requesting practices: a survey study
Authors: Subaveerapandiyan A. , Amees Mohammad , Annamma Lovely M. , Yadav Upasana , Mushanga Kapata 
Published: September 2023

PurposeThis survey-based study aims to explore the research data dissemination and requesting practices of Arab researchers. It investigates the reasons, types, methods, barriers and motivations associated with data sharing and requesting in the Arab research community.Design/methodology/approachA cross-sectional survey was conducted with 205 Arab researchers representing various disciplines and career stages. Descriptive statistics were used for data analysis.FindingsThe study found that 91.2% of Arab researchers share data, while 56.6% access data from others. Reasons for sharing include promoting transparency and collaboration while requesting data is driven by the need to validate findings and explore new research questions. Processed/analysed data and survey/questionnaire data are the most commonly shared and requested types.Originality/valueThis study contributes to the literature by examining data sharing and requesting practices in the Arab research community. It provides original insights into the motivations, barriers and data types shared and requested by Arab researchers. This can inform future research and initiatives to promote regional data sharing.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/OIR-06-2023-0283