bluuebunny/crossref_metadata_2025_split huggingface dataset:
The local community faced with the threat of drought and flooding
Authors: Baron Karolina 
Published: January 2023

ObjectivesThe aim of this study is to analyse the position of local communities towards the risk and the capacity of self-organisation for environmental security in water management in the two areas of drought and flooding. The opinions collected during the questionnaire survey and a review of past behaviour and responses to potential risks and emergencies allowed a diagnosis to be made of the current state of resilience of the communities surveyed to the threat of the water element.MethodsIn June 2022, surveys were carried out using a questionnaire. The data was analysed and graphically processed in the form of charts showing the distribution of answers to the questions asked.ResultsThe results of the research process carried out indicate the current pattern of contacts and cooperation, but also the awareness, knowledge and sense of security of local communities in the face of drought and flood risks. The most important data were collated and this was also related to the nature and specificity of the respondents.ConclusionsThe research undertaken resulted in a discussion on capacity building and the role of citizens in solving the difficulties and challenges of water management in the context of future threats. In addition, a subjective pledge of solutions and tools to strengthen the resilience of social groups to environmental pressures and at the same time to comply with the principle of sustainable and balanced development is included in the conclusions.
Application of Artificial Neural Network Algorithms to Heart Disease Prediction Models with Python Programming
Authors: Yuda Syahidin  , Aditya Pratama Ismail  , Fawwaz Nafis Siraj  
Published: January 2023

Heart disease is one of the deadliest diseases in and is the number one killer in the world so many studies are carried out to contribute to predicting a person's heart disease. This study aims to help create an early heart disease prediction model from the UCI Machine Learning Repository dataset. The method proposed in this study is a deep learning technique that applies an artificial neural network algorithm with a hidden layer technique in making a heart disease prediction model. This research stage found problems in improving the accuracy of the datasets used by dealing with problems in pre-processing data, such as missing data and determining the form of data correlation. The model was then tested through a heart disease dataset and yielded 90% accuracy. With the creation of this prediction model with python programming, it is hoped that in addition to helping to make disease predictions, it can also provide further innovations in data science in the health sector.
Penguatan Peran Pemerintah Desa dalam Mengembangkan Produk Unggulan Desa Melalui Badan Usaha Milik Desa Bintang Baru untuk Meningkatkan Kesejahteraan Masyarakat di Desa Parit Baru
Authors: Yani Riyani  , Rizky Adithya  , Linda Suherma  , Kartawati Mardiah  , Susan Andriana  , Baidhillah Riyadhi  , Oscar Rynandi  , Henri Parsetyo  , Haryati  , Widodo  
Published: January 2023

Desa memperoleh pelimpahan wewenang sejak tahun 2014 sejak adanya Undang Undang tentang desa. Adanya wewnang tersebut desa dapat melakukan pemungut pendapatan daerahnya untuk membiayai aktivitas kegiatan belanja daerahnya yang bertujuan untuk memajukan perekonomian masyarakat desa. Satu diantara desa yang memiliki tujuan tersebut yakni desa Parit Baru. Dalam mencapai tujuan tersebut, desa membentuk suatu badan usaha yang berdomisili di wilayahnya yang diberi nama Badan Usaha Milik Desa Bintang Baru. Pemerintah desa mengontorkan dana penyertaan modal pada tahap awal pendiriannya di tahun 2019 hingga 2 tahun terakhir selanjutnya. PPM dilakukan melalui survei pemetaan masalah yang selanjutnya dilakukan pemecahan masalah dengan cara pengumpulan data perkembangan jumlah pelanggan serta pengeluaran dan pendapatan. Tujuan dilakukannya PPM ini yakni membuat rencana program kerja anggaran badan usaha milik desa Bintang Baru.
Kata Dosa DalamTerjemahan Al-Qur’an Kemenag
Authors: Ana Sri , Nursalam Yufridal Fitri 
Published: January 2023

Kata dosadalampenerjemahan Al-Qur’an Kemenag yang terletakdiberbagaisurat di Al-Qur’an yang ditafsirkan oleh penerjemahdariragam kata yang adadidalam Al-Qur’an,termasuk kata Khoti’ahdan Zhanbun. Sifat terjemahan Al-Qur’an yang tafsirimenuntutpenerjemahlebihmenguraskekuatanbacaandari Tafsir Al-Qur’an.
Tulisan inibertujuanuntukmencarirelasi, bagaimanamuffasirmemaknaiayat yang terdapatkata Khoti’ahdan Zhanbun, dan apakahrelevanjika diterjemahkandengan kata dosadalambahasa Indonesia.
Penelitianiniadalahpenelitiankepustakaan (library research), penulismerujuk (data) pada hasilterjemahan Al-Qur’an Kemenag. Kemudiandidukungdari kitab-kitab tafsir untukmengetahui tafsir terkaitayat dan kata yang akandianalisa, juga menggunakanbuku, jurnal dan literatur yang berkaitandenganjudul yang diambil oleh penulis. Data-data selanjutnyadianalisaperbedaansetiaplafazberdasarkanragampenafsiran.
Setelah menganalisa kata Khotoya dan zhanbundari para mufassiryangditerjemahkandosa oleh TerjemahanKemenagmenunjukkanbahwaTerjemahanKemenagmencobamemaknai dan menafsiridua kata tersebutsebagaimana yang dikehendaki oleh Al-Qur’an.Hal initerjadikarenaketerbatasan kata yang tepatdalambahasaIndonesialah yang menyebabkan kata dosadipilihuntukmenyampaikanpesantersebut.
PET/CT en estadiaje y evaluación del tratamiento en cáncer de pulmón de células no pequeñas
Authors: Guzmán-Barrón Rosanna Morales , Amorín Kajatt Edgar , Ledesma Vásquez Richard , Casavilca Zambrano Sandro 
Published: January 2023

El cáncer de pulmón constituye un problema de salud en el Perú, siendo el más agresivo en su comportamiento. Afecta por igual a hombres y mujeres. El PET/CT constituye una herramienta útil en el estadiaje y seguimiento del tratamiento de estos tumores. Se tiene en la actualidad evidencias suficientes para afirmar que esta tecnología permite adecuar el tratamiento y tener influencia en la sobrevida a largo plazo. En este trabajo se ha realizado una revisión descriptiva de los artículos relevantes publicados recientemente sobre el tema. Se concluye que el estudio PET/CT es útil en el estadiaje y seguimiento del tratamiento propuesto para los pacientes con cáncer de pulmón de células no pequeñas. Se tiene además la información que este tumor maligno sigue llegando a las consultas médicas en estadio.
Estudio sobre prevalencia de Streamings educativos en tiempos de pandemia en los docentes municipales del DMQ.
Authors: Farinango Tayo Christian , Vallejos Caizachana Freddy 
Published: January 2023

La transmisión de contenido multimedia por internet o Streamings ha existido desde que se popularizó en plataformas dedicadas a compartir videos, a nivel mundial universidades de prestigio lo utilizan como instrumento académico para impartir conocimientos a los estudiantes por sus múltiples ventajas, en Ecuador pese a que la herramienta es conocida no es muy utilizada en el medio docentes debido a su desconocimiento, en otros casos utilizan videos ajenos a su autoría y usan metodologías tradicionales en sus producciones audiovisuales; con la llegada de la pandemia COVID-19 se evidenció una prevalencia de su uso para fines didácticos. El desafío para la generación de docentes es crear su propio contenido audiovisual utilizando metodologías activas para su PEA.
El objetivo es determinar la forma adecuada para la realización de un Streamings educativo a fin de orientar el accionar de los profesionales de la educación, los resultados obtenidos muestran que no se está realizando Streamings educativo en ninguna plataforma de internet sin embargo consideran que se debe realizar Streamings de alta calidad ya que su utilización es positiva para su accionar educativo, se observa una considerable inversión en conexión a internet fijo y móvil y finalmente trabajan con Streamings de otros autores.
Genetics in the NICU: Nurses' Perceived Knowledge and Desired Education
Authors: Shields Kathleen , Czerwinski Jennifer , Dauwe Tracie , Hashmi Syed , Hillman Paul , Mowrey Kate , Gunther Kathryn 
Published: January 2023

Background
Many infants admitted to the neonatal intensive care unit (NICU) have genetic conditions. Previous research has shown that gaps exist in the genetics knowledge of nurses and that they lack comfort applying genetics information to clinical practice. Studies assessing the knowledge or comfort of NICU nurses with genetics have not previously been completed.

Method
A total of 122 NICU nurses completed a survey assessing perceived knowledge of genetics, comfort with clinical scenarios involving genetics, and desired genetics education.

Results

Perceived knowledge and overall comfort were correlated with highest degree received, how prepared a nurse felt by the genetics education received in their training, and having a close relationship with someone with a genetic condition. Almost all respondents (96%,
n
= 117) desired additional genetics education.

Conclusion

Gaps exist in the genetics knowledge of neonatal nurses in our cohort, and their overall comfort working with clinical scenarios involving genetics was low. There is significant interest in additional genetics education.

[
J Contin Educ Nurs
. 2023;54(1):16–24.]
Fibrotic Lung Disease Alters Neutrophil Trafficking and Promotes Neutrophil Elastase and Extracellular Trap Release
Authors: Warheit-Niemi Helen I , Huizinga Gabrielle P , Edwards Summer J , Wang Yizhou , Murray Susan K , O’Dwyer David N , Moore Bethany B 
Published: January 2023

Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible disease characterized by collagen deposition within the interstitium of the lung. This impairs gas exchange and results in eventual respiratory failure. Clinical studies show a correlation between elevated neutrophil numbers and IPF disease progression; however, the mechanistic roles neutrophils play in this disease are not well described. In the present study, we describe alterations to the trafficking and function of neutrophils after the development of fibrosis. We observed increased numbers of total and aged neutrophils in peripheral tissues of fibrotic mice. This appeared to be driven by an upregulation of neutrophil chemokine Cxcl2 by lung cells. In addition, neutrophil recruitment back to the bone marrow for clearance appeared to be impaired, because we saw decreased aged neutrophils in the bone marrow of fibrotic mice. Neutrophils in fibrosis were activated, because ex vivo assays showed increased elastase and extracellular trap release by neutrophils from fibrotic mice. This likely mediated disease exacerbation, because mice exhibiting a progressive disease phenotype with greater weight loss and mortality had more activated neutrophils and increased levels of extracellular DNA present in their lungs than did mice with a nonprogressive disease phenotype. These findings further our understanding of the dynamics of neutrophil populations and their trafficking in progressive fibrotic lung disease and may help inform treatments targeting neutrophil function for patients with IPF experiencing disease exacerbation in the future.
The Lack of Natural IgM Increases Susceptibility and Impairs Anti-Vi Polysaccharide IgG Responses in a Mouse Model of Typhoid
Authors: Alugupalli Akhil S , Cravens Matthew P , Walker Justin A , Gulandijany Dania , Dickinson Gregory S , Debes Gudrun F , Schifferli Dieter M , Bäumler Andreas J , Alugupalli Kishore R 
Published: January 2023

Abstract
Circulating IgM present in the body prior to any apparent Ag exposure is referred to as natural IgM. Natural IgM provides protective immunity against a variety of pathogens. Salmonella enterica serovar Typhi (S. Typhi) is the causative agent of typhoid fever in humans. Because mice are not permissive to S. Typhi infection, we employed a murine model of typhoid using S. enterica serovar Typhimurium expressing the Vi polysaccharide (ViPS) of S. Typhi (S. Typhimurium strain RC60) to evaluate the role of natural IgM in pathogenesis. We found that natural mouse IgM binds to S. Typhi and S. Typhimurium. The severity of S. Typhimurium infection in mice is dependent on presence of the natural resistance-associated macrophage protein 1 (Nramp1) allele; therefore, we infected mice deficient in secreted form of IgM (sIgM) on either a Nramp1-resistant (129S) or -susceptible (C57BL/6J) background. We found that the lack of natural IgM results in a significantly increased susceptibility and an exaggerated liver pathology regardless of the route of infection or the Nramp1 allele. Reconstitution of sIgM−/− mice with normal mouse serum or purified polyclonal IgM restored the resistance to that of sIgM+/+ mice. Furthermore, immunization of sIgM−/− mice with heat-killed S. Typhi induced a significantly reduced anti-ViPS IgG and complement-dependent bactericidal activity against S. Typhi in vitro, compared with that of sIgM+/+ mice. These findings indicate that natural IgM is an important factor in reducing the typhoid severity and inducing an optimal anti-ViPS IgG response to vaccination.
Microarray analysis reveals novel insights into immunity to H1N1/09 influenza vaccine in aviremic HIV infected children and adolescents in IMPAACT P1088 (VIR2P.1024)
Authors: de Armas Lesley , George Varghese , Steel Courtney , Filali Ali , Wilkinson Peter , Parmigiani Anita , Andreansky Samita , Gonzalez Ivan , Weinberg Adriana , Cunningham Coleen , Trautmann Lydie , Sekaly Rafick-Pierre , Cameron Mark , Pahwa Savita 
Published: January 2023

Abstract
HIV infected persons often exhibit poor responses to influenza vaccine despite effective HAART-mediated viral suppression. The goal of the present study was to identify gene expression profiles that characterize vaccine driven antibody responses in aviremic pediatric and adolescent HIV infected patients (4-25 yrs) given two doses of Novartis/H1N1 09 vaccine 21 days apart during the pandemic H1N1 influenza outbreak in 2009. Blood samples were collected in Paxgene tubes on days 0, 21, and 31 post-vaccination for microarray analysis. Supervised clustering analysis for contrasts between vaccine responders (R) and vaccine non-responders (NR) was performed and top pathways were ranked by Ingenuity Pathway analysis. Cutoffs for fold change (&lt;-1.3, &gt;1.3) and p value (0.05) were applied to assess the top upregulated and downregulated genes in pathways. Patients were segregated by age into 2 groups for analysis: 1) &lt;12 yrs, n=16 and 2) ≥13 yrs, n=28. In group 1, R showed upregulation of genes related to cell growth and metabolism at both d0 and d21. At d21, B cell differentiation, cell survival and stress response pathways were also upregulated. In group 2, R showed upregulation of innate signaling pathways at d0, and at d21 showed reduced TNF and upregulation of genes involved in T helper differentiation and B cell signaling pathways. Our results reveal signatures of successful vaccine response in HIV infected subjects and may identify favorable targets for improved vaccine design.
Identifying highly conserved and immunogenic SARS-CoV-2 CD8+ T Cell Epitopes for Vaccine Design
Authors: Carmona Jacqueline , Wilson Eric A , Yuvaraj Padhmavathy , Ulrich Peaches R , Singharoy Abhishek , Anderson Karen S 
Published: January 2023

Abstract
Vaccines to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induce antibody responses against the spike (S) protein. However, the continued emergence of variants such as Omicron (B.1.1.529), with 30 amino acid changes in the S protein, underscores the need to find conserved targets that induce adaptive immunity. Natural infection induces CD8+ T cell activation against the viral proteins S, membrane (M), and nucleocapsid (N) as well as NSPs (non-structural proteins). Our study seeks to find conserved and immunogenic MHC class I epitopes throughout the SARS-CoV-2 peptidome. We used Ensemble MHC, a novel consensus predictive algorithm to select highly immunogenic CD8+ T cell epitopes presented by 52 of the most common HLA-I alleles, covering &gt; 98% of the world’s population. We selected 658 high confidence predicted peptides from the SARS-CoV-2 immunopeptidome and prioritized testing 108 structural epitopes derived from the S, M, N, and E proteins. We collected PBMCs from unexposed, healthy, and convalescent individuals (n=31) covering ~ 85% of HLA alleles in the population, and measured T cell immunity by IFNγ secretion ELISpot and Activation Induced Marker (AIM) flow cytometry. In convalescent individuals, all predicted epitopes tested so far (n=36) were immunogenic (IFNγ response &gt;2 fold than background) but 3/19 tested donors were unreactive. Samples from pre-pandemic (2017–19) unexposed healthy donors are reactive to 42% of tested epitopes, which indicate cross-reactivity to common cold coronaviruses (CCC) and possibly pre-existing protection against SARS-CoV-2. In summary, we have identified 45 highly conserved and immunogenic SARS-CoV-2 CD8+ T cell epitopes as potential targets for vaccine design.
Supported by a grant from the Arizona Department of Health Services (AZDHS)
HDAC6 knockout alleviates pristane-induced lupus
Authors: Xu Dao , Reilly Christopher M 
Published: January 2023

Abstract
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by excessive inflammation and production of pathogenic antibodies. Histone deacetylase 6 (HDAC6) is a class IIb histone deacetylase. It has been reported that selective HDAC6 inhibition decreases inflammation in lupus mice models. C57BL/6 mice develop SLE-like symptoms following pristane injection. In this study, sex and age-matched wild type and HDAC6−/− mice on the C57BL/6 background were administered 0.5 ml pristane or PBS intraperitoneally (i.p.) at 8–12 weeks-of-age and were euthanized 10 days later. At sacrifice, body weight and spleen weight were measured, sera were collected, splenocytes and peritoneal cells were harvested for flow cytometry. We found pristane administration increased the spleen weight with no difference between WT mice and HDAC6−/− mice. Flow cytometry results showed that there was no difference in T cell or B cell populations in the spleen. Pristane administration promoted the population of CD11b+ Ly6Chigh inflammatory monocytes and CD11b+ Ly6G+ neutrophils. Peritoneal recruitment of these inflammatory monocytes and neutrophils in HDAC6−/− mice was significantly decreased compared to the WT mice. Pristane administration also induced the interferon (IFN) signature genes, like Mx1, Oas1a, Irf7, Irf9, Cxcl10, Isg15. qRT-PCR revealed that these IFN signature genes were decreased in HDAC6−/− mice compared to the WT mice. In summary, our results show that HDAC6 knockout inhibits the recruitment of inflammatory monocytes and neutrophils in the peritoneum in early inflammation response to pristane. HDAC6 deletion also inhibited the expression of IFN signature genes with pristine stimulation.
3D-IBEX: Achieving multiplex 3-dimensional imaging for deep phenotyping of cells in tissues
Authors: Arroyo-Mejías Armando Javier , Ichise Hiroshi , Chu Colin , Hor Jyh Liang , Yaniv Ziv , Kabat Juraj , Croteau Joshua , Lowekamp Bradley , Radtke Andrea J , Germain Ronald N 
Published: January 2023

Abstract
2D multiplexed antibody-based imaging provides a framework to study cell-cell interactions in thin tissue sections but lacks the ability to interrogate spatial relationships in larger 3D anatomical structures. Several methods for 3D imaging of optically cleared tissue exist, but current approaches do not provide the marker depth afforded by high content 2D imaging. To overcome this constraint, we combined a fast hydrophilic tissue clearing technique, clearing-enhanced 3D (Ce3D), with the recently developed Iterative Bleaching Extends Multiplexity (IBEX) technique, and created 3D-IBEX. 3D-IBEX offers a high-resolution multiplex 3D imaging method. It has been used to analyze murine lung, lymph nodes, retina, and cornea as well as human retina and jejunum, yielding seamless views of large-scale tissue structures while obtaining single-cell resolution of multiple markers, permitting the identification of discrete cell subsets and structures. To date, up to 15 markers have been visualized in a single sample using 3 iterative staining and imaging cycles. This represents a major advance in the emerging field of multiplexed high-resolution 3D imaging but requires further refinements to address issues such as slow antibody penetration, the need for new computational algorithms to process large datasets, and implementation of methods for optimal registration of iterative images. When fully realized, 3D-IBEX will open new avenues for characterization of rare cell subsets missed in thin tissue sections, visualization of structures like nerves and vessels whose trajectories in a tissue are disrupted by sectioning, and delineation of mesoscale tissue domains that contribute to homeostasis and are often the substrates for pathology.
Intramural NIAID Research Opportunities (INRO) program.
Host genetics influence response to targeted immunotherapy and effector cell capacity to perform key immune functions
Authors: Glassbrook James E , Hackett Justin , Muñiz Maria , Dyson Gregory , Gibson Heather 
Published: January 2023

Abstract
Targeted immunotherapies have revolutionized cancer treatment, markedly improving patient cancer outcomes. Despite this rise to prominence as first line treatments for various malignancies, only a subset of patients respond to targeted immunotherapy. Trastuzumab targets tumor associated antigen (TAA) epidermal growth factor 2 (HER2). Differential response to trastuzumab suggests host genetics may influence response to treatment. To model this interaction, we utilize a genetically heterogeneous Diversity Outbred (DO) mouse model in an F1 cross (DOxBALB/c)F1. These genetically diverse mice (n=126) were orthotopically implanted with TUBO (BALB/c-syngeneic tumor) and treated with αNeu (7.16.4) mAb therapy. Like the human population, we observe a divergent response to therapy. Via genetic linkage analysis, we identify loci that correlate to a robust response. We confirm these loci in vivo using recombinant inbred collaborative cross (CC) strains containing/lacking driver loci (CCxBALB/c)F1. These loci alone are not sufficient to fully account for durable response to therapy; however, here we identify strains that uniformly eliminate tumor and conversely, are consistently refractory to treatment. Utilizing tissues from these strains, we evaluate canonical mechanisms of mAb therapy and show that tumor doubling time correlates with bone marrow derived macrophage capacity to phagocytose opsonized tumor in vitro via confocal microscopy. To better understand which cell populations of tumor infiltrate are expressing transcripts from these loci, we perform transcriptomic analysis on putative responder/non-responder strains, allowing us to see key differences in tumor infiltrating immune cell populations.
Supported by NIH R37 CA220482, DMC Foundation, P30 CA022453