bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digitaler Zwilling Brücke im Betrieb – Mögliche Anwendungsfälle
Authors: Nieborowski Sonja , Windmann Sarah , Bednorz Jennifer , Hindersmann Iris , Zinke Tim 
Published: January 2023

AbstractDer Digitale Zwilling Brücke leistet einen wichtigen Beitrag auf dem Weg zum prädiktiven Lebenszyklusmanagement. In der Lebenszyklusphase „Betrieb“ kann der Digitale Zwilling Brücke als effizientes Werkzeug in Bezug auf Analysieren, Vorhersagen, Steuern und Überwachen dienen. Ziel ist eine optimierte Erhaltung, deren Bedeutung angesichts der aktuellen Herausforderungen für Ingenieurbauwerke weiter steigt. Relevante Stakeholder mit ihren Bedarfen und Anforderungen müssen frühzeitig in die Konzeption des Digitalen Zwillings Brücke eingebunden werden. Dieser Weg wird durch die Bundesanstalt für Straßenwesen (BASt) in vielfältigen Forschungsprojekten verfolgt und auch in einem laufenden Projekt zur Entwicklung einer Gesamtkonzeption des Digitalen Zwillings Brücke fortgeführt. Mögliche Anwendungsfälle wurden gesammelt und den Themenbereichen „Betriebsprozesse“, „Erhaltungsplanung und ‐durchführung“ sowie „Strategisches Lebenszyklusmanagement“ zugeordnet. Im Rahmen eines Workshops dienten die Anwendungsfälle der Diskussion und zur initialen Priorisierung mit Stakeholdern. Die Ergebnisse bilden eine wichtige Grundlage für die Ausarbeitung des modularen Gesamtkonzepts Digitaler Zwilling Brücke und den Weg in die Praxis.
Ermittlung thermischer Untergrundeigenschaften zur Optimierung der Kabeldimensionierung
Authors: Roller Fabian , Kleiber Stephanie , Drefke Christoph , Stegner Johannes , Meier Claas 
Published: January 2023

AbstractDie Dimensionierung von Höchstspannungs‐Gleichstrom‐Übertragungsleitungen (HGÜ) basiert auf detaillierten Kenntnissen des Baugrunds im Hinblick auf die Wärmeleitfähigkeit. Aufgrund dessen wurde für die Realisierung der Windstromleitung SuedLink mit einer Trassenlänge von ca. 700 km ein Messprogramm entwickelt, das eine sehr große Anzahl an reproduzierbaren Wärmeleitfähigkeitsuntersuchungen ermöglicht. Mithilfe eines hocheffizienten Versuchsstands können damit vom gesättigten bis zum ofentrockenen Zustand einer Bodenprobe die korrespondierenden Wärmeleitfähigkeitswerte ermittelt werden. Die Auswertung der Wärmeleitfähigkeits‐Wassergehalts‐Beziehung wurde maßgeblich weiterentwickelt, wobei die Steinanteile des Bodens und die Grundwasserverhältnisse Berücksichtigung finden. Die daraus gewonnenen Erkenntnisse fließen in die Planung der Kabeldimensionierung zur Optimierung von Kabelachsabständen ein und tragen somit zur Minimierung der Projektkosten und der Eingriffe in die Natur bei. Darüber hinaus dienen die zahlreichen Messergebnisse der Erweiterung bestehender Datenbanken für Wärmeleitfähigkeitswerte, um für zukünftige Erdkabelprojekte über sog. Pedotransferfunktionen die Anzahl der benötigten Messungen zu verringern.
Metabolic engineering of betacyanin in vegetables for anti‐inflammatory therapy
Authors: Saito Shiori , Nishihara Masahiro , Kohakura Masato , Kimura Kosuke , Yashiro Takuya , Takasawa Seidai , Arimura Gen‐ichiro 
Published: January 2023

AbstractBetalains, which consist of the subgroups betaxanthins and betacyanins, are hydrophilic pigments that have classically been used for food colorants. Owing to their strong antioxidant property, their usefulness for application for therapeutic use is also expected. In addition, as betalains are mainly naturally available from plants of the order Caryophyllales, including beet (Beta vulgaris), metabolic engineering for betalain production in crops such as vegetables, fruits and cereals may provide new food resources useful for healthcare. Here we conducted metabolic engineering of betacyanins in tomato fruits and potato tubers. The transgenic tomato fruits and potato tubers with coexpression of betacyanin biosynthesis genes, CYP76AD1 from B. vulgaris, DOD (DOPA 4,5‐dioxygenase) and 5GT (cyclo‐DOPA 5‐O‐glucosyltransferase) from Mirabilis jalapa, under control of suitable specific promoters, possessed dark red tissues with enriched accumulation of betacyanins (betanin and isobetanin). The anti‐inflammatory activity of transgenic tomato fruit extract was superior to that of wild‐type fruit extract on macrophage RAW264.7 cells stimulated with lipopolysaccharide (LPS), as a result of decreased LPS‐stimulated transcript levels of proinflammatory genes. These findings were in accord with the observation that administration of the transgenic tomato fruits ameliorated dextran sulfate sodium (DSS)‐induced colitis as well as body weight loss and disease activity index in mice, via suppression of DSS‐stimulated transcript levels of pro‐inflammatory genes, including Tnf (encoding TNF‐alpha), Il6, and Ptgs2 (encoding cyclooxygenae 2). Intriguingly, given the fact that the transgenic potato tuber extract failed to enrich the anti‐inflammatory activity of macrophage cells, it is likely that metabolic engineering of betacyanins will be a powerful way of increasing the anti‐inflammatory property of ordinary foods such as tomato.
Functional near‐infrared spectroscopy measures of neural activity in children with and without developmental language disorder during a working memory task
Authors: Hancock Allison S. , Warren Christopher M. , Barrett Tyson S. , Bolton David A. E. , Gillam Ronald B. 
Published: January 2023

AbstractIntroductionChildren with developmental language disorder (DLD) exhibit cognitive deficits that interfere with their ability to learn language. Little is known about the functional neuroanatomical differences between children developing typically (TD) and children with DLD.MethodsUsing functional near‐infrared spectroscopy, we recorded oxygenated hemoglobin (O2hb) concentration values associated with neural activity in children with and without DLD during an auditory N‐back task that included 0‐back, 1‐back, and 2‐back conditions. Analyses focused on the left dorsolateral prefrontal cortex (DLPFC) and left inferior parietal lobule (IPL). Multilevel models were constructed with accuracy, response time, and O2hb as outcome measures, with 0‐back outcomes as fixed effects to control for sustained attention.ResultsChildren with DLD were significantly less accurate than their TD peers at both the 1‐back and 2‐back tasks, and they demonstrated slower response times during 2‐back. In addition, children in the TD group demonstrated significantly greater sensitivity to increased task difficulty, showing increased O2hb to the IPL during 1‐back and to the DLPFC during the 2‐back, whereas the DLD group did not. A secondary analysis revealed that higher O2hb in the DLPFC predicted better task accuracy across groups.ConclusionWhen task difficulty increased, children with DLD failed to recruit the DLPFC for monitoring information and the IPL for processing information. Reduced memory capacity and reduced engagement likely contribute to the language learning difficulties of children with DLD.
Strategic outcome of competitive advantage from corporate sustainability practices: Institutional theory perspective from an emerging economy
Authors: Bhuiyan Faruk , Rana Tarek , Baird Kevin , Munir Rahat 
Published: January 2023

AbstractThis study examined the use and effectiveness of corporate sustainability practices (CSP) and the subsequent effect on a strategic outcome, competitive advantage. The new institutional sociology (NIS) theoretical framework was applied, informed by three different dimensions of institutional pressures (coercive, mimetic and normative). The study used a survey method and developed a seven‐dimensional model utilising the 52 principles provided by the OECD. It used a structural equation modelling in order to test the hypothesised associations between institutional pressures, CSP and competitive advantage to provide an institutional and contextualised perspectives from an emerging economy setting. The study found significant associations between the three types of institutional pressure with specific dimensions of CSP. The findings further revealed that specific CSP dimensions are diversely (positively and negatively) associated with competitive advantage. In line with the tenets of greenwashing, it highlighted the important role of institutional pressures from stakeholders (government, policy and customers) in implementing specific CSP. The findings inform managers, governments, foreign investors and other stakeholders in emerging economies about the influence of the institutional pressure in promoting the use of CSP and the effect of such practices on competitive advantage. From the context of an emerging economy, the study provides a unique empirical insight into the NIS perspective in promoting CSP and the subsequent impact on the strategic outcome, competitive advantage.
Modified one‐stage technique of laterally positioned flap with subepithelial connective tissue graft for the treatment of peri‐implant soft tissue dehiscence in the esthetic zone: A 5‐year follow‐up
Authors: de Almeida Juliano Milanezi , de Sá Daniela Pereira , Furquim Elisa Mara de Abreu , Matheus Henrique Rinaldi 
Published: January 2023

AbstractBackgroundThe state of art of tissue grafting allows significant improvements in the soft tissue phenotype. The importance of appropriate soft tissue phenotype around implants is supported by recent literature. The present case report aims to describe the application of a modified one‐stage technique of laterally positioned flap with subepithelial connective tissue graft (CTG) for the treatment of peri‐implant soft tissue dehiscence in the esthetic zone.Methods and ResultsA 38‐year‐old female presented 4 years following prosthetic restoration, with a localized soft tissue defect in height and thickness at the buccal aspect of the implant at #11, and the abutment exposed to the oral cavity. Incisions with internal and external bevels were performed in the medial and distal margins of the dehiscence, respectively, toward the alveolar mucosa. After intrasulcular incision, the area was de‐epithelialized and a split thickness flap from mesial #11 to distal #14 was elevated. A tunnel was prepared at #21. CTG was stabilized mesially, within the tunnel prepared, and distally, through simple interrupted sutures. Vertical compressive sutures were performed on the CTG. The flap was laterally positioned and secured by means of suspended sutures. Healing was uneventful, increased thickness and height of the peri‐implant mucosa were observed, with great esthetic outcome. The soft tissue margin was stable at the 5‐year follow‐up.ConclusionThe technique described in this case report showed promising results for covering exposed implant abutment in the esthetic zone, as well as for modification of the soft tissue phenotype around dental implants.Key pointsWhy is this case new information

A modified one‐stage technique that allows coverage of exposed metallic displays of titanium implants while modifying the soft tissue phenotype in the esthetic zone.What are the keys to successful management of this case?

Meticulous incisions and internal and external bevels. Adequate elevation to allow repositioning without tension.
Adequate graft size that extends through all the recipient bed; and tension‐free suture.
What are the primary limitations to success in this case?

Presence of an implant installed in a non‐satisfactory three‐dimensional position.
And poor hygiene and plaque accumulation postoperatively.
The expression of anti‐aging protein Klotho is increased during neural differentiation of bone marrow‐derived mesenchymal stem cells
Authors: Naeeni Bahareh , Taha Masoumeh Fakhr , Emami Aleagha Mohamad Sajad , Allameh Abdolamir 
Published: January 2023

AbstractKlotho, as an antiaging protein, is involved in the maintenance and differentiation of neuronal or glial cells and, therefore, has been noticed as a potential therapeutic target for neurodegenerative disorders. Expression of Klotho has been examined in different cells and organs, however, our information about the developmental pattern of this protein during differentiation of mesenchymal stem cells (MSCs) into neuron‐like cells is limited. In this study, we conducted neural differentiation of mouse bone marrow‐derived‐MSCs and monitored the expression of Klotho together with selected neuron‐specific genes at messenger RNA (mRNA) on days 7 and 14 of differentiation using quantitative real‐time PCR. In addition, Klotho status at protein level was evaluated by immunocytochemistry. The results showed a significant change in the morphology of MSCs towards neuron‐like cells. These changes were observed with progressive growth and formation of cell connections towards the formation of a chain of neuron‐like cells which occurred in the second week of differentiation. Morphological changes were associated with a significant increase in the expression of neuron‐specific genes like pax‐6, neuN and, neurofilaments (NfL). Likewise, there was an increased expression of Klotho mRNA, and accumulation of Klotho protein in neuronal cell bodies, during the cellular differentiation of MSCs. These findings provided new evidence that neuronal differentiation from the MSCs is associated with increased expression of Klotho. These data may provide insight into the importance of Klotho protein in stem cell differentiation and regeneration in response to cell death in the central nervous system.
Proteomics Update and Perspectives from the Proteomics in Cell Biology and Disease Mechanisms Conference
Authors: Kotliar Ilana B. 
Published: January 2023

AbstractProteomics, or the large‐scale study of proteomes, has benefitted from many recent advances in chemical biology, mass spectrometry, and machine learning. The Proteomics in Cell Biology and Disease Mechanisms conference showcased the synergy between these elements and the vast range of biological questions that proteomics can now help us to answer.
EFMC: Trends in Medicinal Chemistry and Chemical Biology
Authors: Auberson Yves P. , Arimondo Paola B. , Duca Maria , Essig Sebastian , Grether Uwe , Rufer Arne C. , Sbardella Gianluca , Schopfer Ulrich , Torrens Antoni , van der Stelt Mario , Vauzeilles Boris , Vázquez Olalla , Zhang Andrew X. 
Published: January 2023

AbstractGround‐breaking research in disease biology and continuous efforts in method development have uncovered a range of potential new drug targets. Increasingly, the drug discovery process is informed by technologies involving chemical probes as tools. Applications for chemical probes comprise target identification and assessment, as well as the qualification of small molecules as chemical starting points and drug candidates. Progress in probe chemistry has opened the way to novel assay formats and pharmaceutical compound classes. The European Federation of Medicinal Chemistry and Chemical Biology (EFMC) has launched the Chemical Biology Initiative to advance science in the field of medicinal chemistry and chemical biology, while representing all members of this extended scientific community. This review provides an overview of the many important developments in the field of chemical biology that have happened at the lively interface of academic and industrial research.
Analyzing RNA‐Protein Interactions by Cross‐Link Rates and CLIP‐seq Libraries
Authors: Porter Douglas F. , Garg Raghav M. , Meyers Robin M. , Miao Weili , Ducoli Luca , Zarnegar Brian J. , Khavari Paul A. 
Published: January 2023

AbstractUV cross‐linking‐based methods are the most common tool to explore in vivo RNA‐protein interactions. UV cross‐linking enables the freezing of direct interactions in the cell, which can then be mapped by high‐throughput sequencing through a family of methods termed CLIP‐seq. CLIP‐seq measures the distribution of cross‐link events by purifying a protein of interest and sequencing the covalently bound RNA fragments. However, there are disagreements and ambiguities as to which proteins are RNA‐binding proteins and what interactions are significant as all proteins contact all RNAs at some frequency. Here we describe a protocol for both determining RNA‐protein interactions through a combination of RNA library preparation and the measurement of absolute cross‐link rates, which helps determine what proteins are RNA‐binding proteins and what interactions are significant. This protocol, comprising an updated form of the easyCLIP protocol, describes guidelines for RNA library preparation, oligo and protein standard construction, and the measurement of cross‐link rates. These methods are easily visualizable through their fluorescent labels and can be adapted to study RNA‐binding properties of both functional, high affinity RNA‐binding proteins, and the accidental RNA interactions of non‐RNA‐binding proteins. © 2023 Wiley Periodicals LLC.Basic Protocol 1: RNA library constructionBasic Protocol 2: Determining UV cross‐link ratesSupport Protocol 1: Cross‐linking and lysing cellsSupport Protocol 2: Adapter preparationSupport Protocol 3: Preparation of cross‐linked RBP standard
Dupilumab provides early and durable improvement of symptoms in patients with chronic rhinosinusitis with nasal polyps
Authors: Gevaert Philippe , Lee Stella E , Settipane Russell A , Wagenmann Martin , Msihid Jérôme , Siddiqui Shahid , Nash Scott , Jacob‐Nara Juby A , Khan Asif H , Kamat Siddhesh , Chuang Chien‐Chia 
Published: January 2023

AbstractObjectivesTo evaluate within‐patient symptom improvement in the dupilumab SINUS‐24/‐52 studies in patients with severe chronic rhinosinusitis with nasal polyps (CRSwNP) (NCT02912468/NCT02898454).MethodsPatients received dupilumab 300 mg or placebo every 2 weeks for 24 (SINUS‐24) or 52 weeks (SINUS‐52) on background intranasal corticosteroids. Patients daily reported symptoms of nasal congestion (NC), loss of smell (LoS) and rhinorrhoea on a scale of 0–3 (0 – no symptoms, 1 – mild, 2 – moderate, 3 – severe symptoms). The proportions of patients with moderate‐to‐severe symptoms (score ≥ 2) at baseline who improved to no‐to‐mild symptoms (score ≤ 1) were determined at Weeks 2, 24 (pooled studies) and 52 (SINUS‐52). Subgroups with prior sinonasal surgery and coexisting asthma were analysed.ResultsAt baseline in the pooled intention‐to‐treat population (n = 724), the proportions of patients with scores ≥ 2 for NC, LoS and rhinorrhoea were 87, 94 and 64%, respectively. Significantly, more patients achieved scores ≤ 1 (no/mild symptoms) with dupilumab vs placebo for each symptom at each time point {Week 2 NC 12% vs 2% [odds ratio 8.9 (95% CI 3.0–26.3)], LoS 5% vs 1% [4.6 (1.3–16.8)], rhinorrhoea 9% vs 2% [4.8 (1.5–15.4)], all P < 0.05; Week 24 NC 54% vs 14% [8.7 (5.6–13.5)], LoS 43% vs 6% [14.4 (7.9–26.0)], rhinorrhoea 53% vs 16% [6.6 (4.1–10.9)], all P < 0.0001}. Results were similar in subgroups with prior surgery and coexisting asthma.ConclusionSignificantly, more patients achieved improvement from moderate‐to‐severe symptoms to no‐to‐mild symptoms with dupilumab than placebo, regardless of prior surgery or coexisting asthma. Improvement was observed as early as Week 2 and continued through to Week 52.
Spatiotemporal predictions of the alternative prey hypothesis: Predator habitat use during decreasing prey abundance
Authors: Brunet Mitchell J. , Monteith Kevin L. , Huggler Katey S. , Thompson Daniel J. , Burke Patrick W. , Zornes Mark , Lionberger Patrick , Valdez Miguel , Holbrook Joseph D. 
Published: January 2023

AbstractThe alternative prey hypothesis supposes that predators supported by a primary prey species will shift to consume alternative prey during a decrease in primary prey abundance. The hypothesis implies that during declines of one prey species, a predator modifies their behavior to exploit a secondary, or alternative, species. Despite occurring in many systems, the behavioral mechanisms (e.g., habitat selection) allowing predators to shift toward alternative prey during declines in the abundance of their primary prey are poorly understood. We evaluated habitat selection and use by a generalist predator with respect to two prey species during a dramatic decrease in the abundance of primary prey. Further, we evaluated how spatial variation in access to primary prey affected habitat selection, and assessed similarity and overlap between habitats used by each prey species. Coyotes (Canis latrans) exhibited decreasing selection for cottontail rabbits (Sylvilagus spp.; primary prey) during population decreases but did not shift habitat selection toward neonate mule deer (Odocoileus hemionus; alternative prey). Use of rabbit habitat remained high even during historically low rabbit abundance, while mule deer habitat was used in proportion to its availability. Coyotes seemingly do not make large shifts in habitat selection toward alternative prey following spatial and temporal decreases in the abundance of primary prey, but instead, take advantage of habitat overlap to facilitate prey‐switching behavior. Our work extends previous research conducted under the alternative prey hypothesis by explicitly evaluating the influence of habitat overlap between prey species and variation in access to prey habitat as factors affecting prey‐switching behaviors in predators.
An Effective Non‐Enzymatic Glucose Biosensor Based on Nanostructured CuxO/Cu Electrodes Synthesized in Situ by Copper Anodization
Authors: Wang Chen , Gao Nan , Gao Zhaoqing , Li Ruofan , Wang Yunpeng , Gong Weitao 
Published: January 2023

AbstractPotentiostatic anodization was developed to synthesize copper oxide/copper (CuxO/Cu, x=1,2) electrode with nano structure for sensitive non‐enzymatic glucose detection. At a catalytic potential of 0.55 V, the CuO/Cu electrode presented a high sensitivity of 2954.38 μA mM−1 cm−2 to glucose and a linear range of 0.1 mM to 1.3 mM. The response time is less than 3 s with addition of 0.1 mM glucose. The CuO/Cu electrode above was anodized in 1M KOH solution at −100 mV and the morphology was compact nanoparticles and sparsely dispersed nanosheets, which enlarged the surface area and provided abundant electrocatalytic active sites. Compared the sensing property of electrodes with different morphologies, it indicated that nanostructure was significant to the efficient glucose catalytic oxidation process and it could be regulated by changing the potential and electrolyte concentration during anodization.
The role of data science in environmental digital twins: In praise of the arrows
Authors: Blair Gordon S. , Henrys Peter A. 
Published: January 2023

AbstractDigital twins are increasingly important in many domains, including for understanding and managing the natural environment. Digital twins of the natural environment are fueled by the unprecedented amounts of environmental data now available from a variety of sources from remote sensing to potentially dense deployment of earth‐based sensors. Because of this, data science techniques inevitably have a crucial role to play in making sense of this complex, highly heterogeneous data. This short article reflects on the role of data science in digital twins of the natural environment, with particular attention on how resultant data models can work alongside the rich legacy of process models that exist in this domain. We seek to unpick the complex two‐way relationship between data and process understanding. By focusing on the interactions, we end up with a template for digital twins that incorporates a rich, highly dynamic learning process with the potential to handle the complexities and emergent behaviors of this important area.