bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacokinetics and Tissue Distribution Study of Daphnoretin in Ethanol
Extract from the Roots of Wikstroemia Indica in Rats by a Validated
UPLC-MS/MS Method
Authors: Wang Wenjing , Feng Guo , Li Lailai , Li Wei , Liu Wen , Wu Zengguang , Su Hongmei , Zhu Guanglin , Ren Chenchen , Song Xueli , Zhang Ju , He Zhengyan 
Published: February 2023

Background:
Daphnoretin, as a known bicoumarin compound that contained various
pharmacological activities, was isolated from Wikstroemia indica C.A. Mey (RWI).

Objective:
The study aims to investigate the pharmacokinetic characteristics of daphnoretin from RWI
ethanol extracts in rat plasma and to determine daphnetin in rat plasma and various tissues by a rapid,
reliable and sensitive ultra high performance liquid chromatography with tandem mass spectrometry
method.

Methods:
The UPLC-MS/MS method was established. Daphnoretin and IS (buspirone) were chromatographed
on an agilent Zorbax XDB-C18 column (2.1 mm × 50 mm, 3.5 μm), and Gradient elution
of acetonitrile-0.15% formic acid in aqueous solution. Quantification was performed using electrospray
ionization in positive ion multiple reaction monitoring mode of the transitions m/z
353.1→179.1 for daphnoretin and m/z 386.3→122.3 for IS.

Results:
Good linearity between 5-10000 ng/mL for cyperidin in plasma and tissue samples (r ≥ 0.99)
was resulted. The accuracies of plasma and tissue homogenates ranged from-3.31% to 9.00%, and the
precision was less than 5.78%. After that, the validated method was successfully applied to the pharmacokinetics
and tissue distribution study of daphnoretin after oral administration of ethanol extract
from the roots of RWI to rats.

Conclusion:
Daphnoretin was well absorbed in the systemic circulation after oral administration and
was widely distributed in tissues, with the highest concentration in lung tissue. This study is beneficial
to the development and utilization of RWI and provides a reasonable reference for its clinical administration.
CCRMDA: MiRNA-disease Association Prediction Based on Cascade
Combination Recommendation Method on a Heterogeneous Network
Authors: Ma Yuan-Lin , Yu Dong-Ling , Liu Ya-Fei , Yu Zu-Guo 
Published: February 2023

Background:
MicroRNAs (miRNAs) are a class of short and endogenous single-stranded
non-coding RNAs, with a length of 21-25nt. Many studies have proved that miRNAs are closely related
to human diseases. Many algorithms based on network structure have been proposed to predict potential
miRNA-disease associations.

Methods:
In this work, a cascade combination method based on network topology is developed to explore
disease-related miRNAs. We name our method as CCRMDA. First, the hybrid recommendation
algorithm is used for a rough recommendation, and then the structural perturbation method is used for a
precise recommendation. A special perturbation set is constructed to predict new miRNA-disease associations
in the miRNA-disease heterogeneous network.

Results:
To verify the effectiveness of CCRMDA, experimental analysis is performed on HMDD V2.0
and V3.2 datasets, respectively. For HMDD V2.0 dataset, CCRMDA is compared with several state-ofthe-
art algorithms based on network structure, and the results show that CCRMDA has the best performance.
The CCRMDA method also achieves excellent performance with an average AUC of 0.953 on
HMDD V3.2 dataset. In addition, case studies further prove the effectiveness of CCRMDA.

Conclusion:
CCRMDA is a reliable method for predicting miRNA-disease.
Drug Repositioning Based on a Multiplex Network by Integrating Disease,
Gene, and Drug Information
Authors: Zhou Gang , Xuan Chenxu , Wang Yan , Zhang Bai , Wu Hanwen , Gao Jie 
Published: February 2023

Background:
The research of new drugs is very expensive and the cycle is relatively long, so it has broad development prospects and good economic benefits to use validated drugs in the treatment of other diseases.

Objective:
The purpose of drug repositioning is to identify other indications for existing drugs. In addition to using disease and drug information for drug repositioning, other biomolecular information can also be integrated for drug repositioning. Integrating multiple biomolecular data of different types can improve the predictive performance of drug repositioning models.

Methods:
This paper proposes a drug repositioning algorithm based on a multiplex network (DRMN algorithm) by integrating disease, gene, and drug information. DRMN algorithm utilizes known disease-gene and gene-drug associations to connect disease phenotype similarity network, gene expression similarity network, and drug response similarity network. Then they are constructed into a multiplex network, and the importance score of each node is calculated by PageRank (PR) algorithm. Finally, disease-drug association scores are sorted to achieve drug repositioning.

Results:
DRMN algorithm is applied to two sets of sample data. Disease-drug association scores are
calculated separately from disease PR values and drug PR values in both datasets. In top 50% of association
scores, lots of disease-drug association prediction results have been verified by existing results.
Compared with other algorithms, DRMN algorithm also shows better performance.

Conclusion:
DRMN algorithm can effectively integrate multi-omics data for drug repositioning and obtain better prediction results.
Application of Polymer Materials in Targeting Glioma
Authors: Guo Jia , Yao Zheng , Zhang Feiyu , Wu Junzi 
Published: February 2023

Abstract:
Glioma is a serious life-threatening disease, and traditional treatments have little effect. In recent decades,
polymer materials have been developed for the treatment of glioma as a new research area. The ability to target reactive
polymeric carriers is important for treating glioma. Polymer materials have good designability and expansibility.
They respond to different stimuli, leading to a change in the macroscopic properties of materials. Sensitive polymer
carriers respond to biological stimuli (pH, oxidative stress, enzyme, temperature, ions and nucleic acids) and the tumour
microenvironment. They can be used as intelligent polymer carriers to transport chemotherapy and imaging drugs
for glioma treatment. The ability of these polymer carriers to control the release of molecules at tumour-specific sites
has aroused great interest. This review summarizes current research on sensitive polymer-carriers for glioma treatment
over the past decade, focusing on their clinical application prospects. Finally, future applications of polymer carriers in
nanomedicine are reviewed.
Osimertinib Induces the Opposite Effect of Proliferation and Migration in the Drug
Resistance of EGFR-T790M Non-small Cell Lung Cancer Cells
Authors: Wang Rou-Hsin , Chang Chien-Jen , Chen Chien-Hung , Liu Kuang-Kai , Chao Jui-I 
Published: February 2023

Background:
Lung cancer has become one of the leading causes of cancer incidence and mortality worldwide.
Non-small cell lung carcinoma (NSCLC) is the most common type among all lung cancer cases. NSCLC patients
contained high levels of activating epidermal growth factor receptor (EGFR) mutations, such as exon 19 deletion,
L858R and T790M. Osimertinib, a third-generation of EGFR tyrosine kinase inhibitor (EGFR-TKI), has therapeutic
efficacy on the EGFR-T790M mutation of NSCLC patients; however, treatment of osimertinib still can induce drug
resistance in lung cancer patients. Therefore, investigation of the drug resistance mechanisms of osimertinib will provide
novel strategies for lung cancer therapy.

Methods:
The H1975OR osimertinib-resistant cell line was established by prolonged exposure with osimertinib derived
from the H1975 cells. The cell proliferation ability was evaluated by the cell viability and cell growth assays. The
cell migration ability was determined by the Boyden chamber assays. The differential gene expression profile was
analyzed by genome-wide RNA sequencing. The protein expression and location were analyzed by western blot and
confocal microscopy.

Results:
In this study, we established the osimertinib-resistant H1975 (T790M/L858R) cancer cells, named the
H1975OR cell line. The cell growth ability was decreased in the H1975OR cells by comparison with the H1975 parental
cells. Conversely, the cell migration ability was elevated in the H1975OR cells. We found the differential gene
expression profile of cell proliferation and migration pathways between the H1975OR and H1975 parental cells. Interestingly,
the protein levels of phospho-EGFR, PD-L1, E-cadherin and β-catenin were decreased, but the survivin and
N-cadherin proteins were increased in the H1975OR drug-resistant cells.

Conclusion:
Osimertinib induces the opposite effect of proliferation and migration in the drug resistance of EGFRT790M
lung cancer cells. We suggest that differential gene and protein expressions in the cell proliferation and migration
pathways may mediate the drug resistance of osimertinib in lung cancer cells. Understanding the molecular drugresistant
mechanisms of proliferation and migration pathways of osimertinib may provide novel targets and strategies
for the clinical treatment of EGFR-TKIs in lung cancer patients.
Stroke and Vascular Cognitive Impairment: The Role of Intestinal
Microbiota Metabolite TMAO
Authors: Tu Ruxin , Xia Jian 
Published: February 2023

Abstract:
The gut microbiome interacts with the brain bidirectionally through the microbiome-gutbrain
axis, which plays a key role in regulating various nervous system pathophysiological processes.
Trimethylamine N-oxide (TMAO) is produced by choline metabolism through intestinal microorganisms,
which can cross the blood-brain barrier to act on the central nervous system. Previous studies
have shown that elevated plasma TMAO concentrations increase the risk of major adverse cardiovascular
events, but there are few studies on TMAO in cerebrovascular disease and vascular cognitive
impairment. This review summarized a decade of research on the impact of TMAO on stroke and related
cognitive impairment, with particular attention to the effects on vascular cognitive disorders. We
demonstrated that TMAO has a marked impact on the occurrence, development, and prognosis of
stroke by regulating cholesterol metabolism, foam cell formation, platelet hyperresponsiveness and
thrombosis, and promoting inflammation and oxidative stress. TMAO can also influence the cognitive
impairment caused by Alzheimer's disease and Parkinson's disease via inducing abnormal aggregation
of key proteins, affecting inflammation and thrombosis. However, although clinical studies have confirmed
the association between the microbiome-gut-brain axis and vascular cognitive impairment (cerebral
small vessel disease and post-stroke cognitive impairment), the molecular mechanism of TMAO
has not been clarified, and TMAO precursors seem to play the opposite role in the process of poststroke
cognitive impairment. In addition, several studies have also reported the possible neuroprotective
effects of TMAO. Existing therapies for these diseases targeted to regulate intestinal flora and its
metabolites have shown good efficacy. TMAO is probably a new target for early prediction and treatment
of stroke and vascular cognitive impairment.
Latest Patents on Buffers
Authors: Liu Hongbo , Zhao Yunqi , Yang Xiao Dong 
Published: February 2023

Background:
When any equipment is running, the physical collision occurs in the machinery,
which requires the hydraulic buffer devices to absorb and dissipate the impact energy in
order to avoid the large vibration and even the damage to the structure caused by the impact load
on the equipment. Therefore, the design of hydraulic buffers is very necessary.

Objective:
Through the analysis of the research status of hydraulic buffers, some valuable conclusions
are extracted from the representative and prospective results to predict the future research and
development direction of hydraulic buffers.

Methods:
The researches on hydraulic buffers are summarized and compared, and the characteristics
of energy absorption and energy consumption of hydraulic buffers are expounded. Combined
with the principle of hydraulic buffers, the related patents of hydraulic buffer are studied.

Results:
By tracing the characteristics of different types of hydraulic buffers, the main problems
existing in the development of hydraulic buffers are analyzed, such as short working life, low energy
absorption efficiency, etc. Solving such problems as a prerequisite, the patents’ development of
the structure and design method of hydraulic buffers is prospected.

Conclusion:
The optimization and development of hydraulic buffers are conducive to improving
the energy absorption efficiency and frequency of hydraulic buffers. This paper, thus, summarizes
the advantages and disadvantages of different kinds of hydraulic buffers by tracing and summarizing
the previous patents, learning that the most widely used buffer is the porous hydraulic buffer,
but the cushioning performance of core rod buffer is more, which is the main development trend in
the future.
Optimization of Single Screw Extrusion Processing Variables and Soy and
Rice Flour Blend Formulations based on Physical Properties of Extrudates
Authors: Mitra Pranabendu , Pakki Srikanth Manohar , Acharya Binu , Khanvilkar Sagar 
Published: February 2023

Background:
Extruded cereals and snacks are mainly carbohydrates. Most adults require
more protein above RDA by restricting carbohydrates for many health benefits. A wide variety of
soy protein-rich extrudates can be produced by blending with rice flour. However, optimum extrusion
processing variables (i.e., screw speed, die temperature, and product formulations) are required
for maximum retention of nutrients with desired product characteristics.

Objective:
The objective of this study was to optimize the extrusion operating conditions and soy
flour and rice flour blend formulations for developing protein-rich cereal-like extrudates.

Methods:
Twenty formulations using CCRD with the combinations of soy flour content (43-77%),
die temperature (123-157°C), and screw speed (250-350 rpm) were extruded using a single screw
extruder. The physical properties (expansion ratio, density, porosity, hardness, crispness, color, and
water solubility index) of extrudates were determined. The regression models and 3-dimensional
response surface models were developed for each property of extrudates using RSM. Numerical and
graphical optimizations were conducted based on the desirability to determine an optimum condition.

Results:
The regression models were able to predict the physical properties of extrudates with an
accuracy of 75-90%, depending on their properties. The 3-dimensional response surface models indicated
that the soy flour content, die temperature, and screw speed affected the physical properties
of extrudates significantly (p < 0.05). Soy flour content (50-55%), die temperature (140-145°C), and
screw speed (290-320 rpm) were very effective for the desired quality of soy flour and rice flour
blend extrudates.

Conclusion:
The optimum condition is expected to be very useful for soy protein and rice flour
blend extruded cereal-like product development commercially within the experimental range.
Microwave-assisted Sustainable Production of Biodiesel: A Comprehensive Review
Authors: Ao Supongsenla , Chhandama Michael VL , Li Hu , Lalthazuala Rokhum Samuel 
Published: February 2023

Abstract:
Limited crude petroleum and growing awareness of fossil fuel depletion have enabled the development of alternative fuels and new energy sources. Biodiesel, also known as fatty acid methyl esters (FAME), has received a lot of attention due to its biodegradability, renewability, cost effective and nontoxicity. The purity of biodiesel production and uniform heating are the major hurdles for large scale biodiesel production. Recent microwave energy-based heating method has proved the potential for cleaner chemical production, short time duration, uniform heating, and purity over conventional heating method. The goal of this review is to discuss the biodiesel production using microwave-assisted heating. The different feedstocks used for biodiesel production, effects of mi-crowave irradiation, factors affecting the rate of microwave-assisted transesterification to produce biodiesel were comprehensively discussed. Microwave irradiation has been compared to other tech-nologies aiming to enhance the efficiency of overall process. The primary knowledge gaps in bio-diesel production can be identified based on this research, ensuring the biodiesel industry's long-term sustainability.
Physical Activity Surveillance in Children and Adolescents Using Smartphone Technology: Systematic Review
Authors: Nasruddin Nur Izzatun Nasriah , Murphy Joey , Armstrong Miranda Elaine Glynis 
Published: February 2023

Background
Self-reported physical activity (PA) questionnaires have traditionally been used for PA surveillance in children and adolescents, especially in free-living conditions. Objective measures are more accurate at measuring PA, but high cost often creates a barrier for their use in low- and middle-income settings. The advent of smartphone technology has greatly influenced mobile health and has offered new opportunities in health research, including PA surveillance.

Objective
This review aimed to systematically explore the use of smartphone technology for PA surveillance in children and adolescents, specifically focusing on the use of smartphone apps.

Methods
A literature search was conducted using 5 databases (PubMed, Scopus, CINAHL, MEDLINE, and Web of Science) and Google Scholar to identify articles relevant to the topic that were published from 2008 to 2023. Articles were included if they included children and adolescents within the age range of 5 to 18 years; used smartphone technology as PA surveillance; had PA behavioral outcomes such as energy expenditure, step count, and PA levels; were written in English; and were published between 2008 and 2023.

Results
We identified and analyzed 8 studies (5 cross-sectional studies and 3 cohort studies). All participants were aged 12-18 years, and all studies were conducted in high-income countries only. Participants were recruited from schools, primary care facilities, and voluntarily. Five studies used mobile apps specifically and purposely developed for the study, whereas 3 studies used mobile apps downloadable from the Apple App Store and Android Play Store. PA surveillance using these apps was conducted from 24 hours to 4 weeks.

Conclusions
Evidence of PA surveillance using smartphone technology in children and adolescents was insufficient, which demonstrated the knowledge gap. Additional research is needed to further study the feasibility and validity of smartphone apps for PA surveillance among children and adolescents, especially in low- and middle-income countries.
The Indigenous Australian Human Papillomavirus (HPV) Cohort Study 2, Continuation for 5 to 10 Years: Protocol for a Longitudinal Study
Authors: Hedges Joanne , Sethi Sneha , Garvey Gail , Whop Lisa J , Canfell Karen , Dodd Zell , Larkins Priscilla , Antonsson Annika , Smith Megan A , Mittinty Murthy , Leane Catherine , Reid Nicolas , Ooi Eng H , Ju Xiangqun , Logan Richard , Jamieson Lisa 
Published: February 2023

Background
Human papillomavirus (HPV) infection, a common sexually transmitted disease, is associated with cancers of the cervix, vulva, vagina, penis, anus, and head and neck. Oropharyngeal squamous cell carcinoma (OPSCC; throat cancer) is a type of cancer involving the head and neck area that is rapidly increasing across the globe. There are higher rates of OPSCC among Indigenous populations relative to non–Indigenous Australian populations, although the HPV-attributable fraction remains unknown. For the first time at a global level, we plan to extend an Indigenous Australian adult cohort to monitor, screen, and ultimately prevent HPV-associated OPSCC and to undertake extensive cost-effectiveness modelling around HPV vaccination.

Objective
This study aims to (1) extend follow-up to a minimum of 7 years post recruitment to describe the prevalence, incidence, clearance, and persistence of oral HPV infection; and (2) conduct clinical examinations of the head and neck, oral cavity, and oropharynx and collect saliva samples for early-stage OPSCC testing.

Methods
We will continue to implement a longitudinal design for the next study phase, where we will ascertain the prevalence, incidence, clearance, and persistence of oral HPV infection at 48, 60, and 72 months; undertake clinical examinations/saliva assessments to detect early-stage OPSCC; and refer for treatment. The primary outcome measures are changes in oral HPV infection status, biomarker measures of early HPV-related cancer, and clinical evidence of early-stage OPSCC.

Results
Participant 48-month follow-up will commence in January 2023. The first results are expected to be submitted for publication 1 year after 48-month follow-up begins.

Conclusions
Our findings have potential to change the way in which OPSCC among Australian Indigenous adults is managed, with desired impacts including cost-savings on expensive cancer treatments; improved nutritional, social, and emotional outcomes; and improved quality of life for both Indigenous adults and the Indigenous community more broadly. Continuing a large, representative Indigenous adult cohort to track oral HPV infection and monitor early OPSCC is essential to yield critical information to include in the management armamentarium of health and well-being recommendations for Australia’s First Nations.

International Registered Report Identifier (IRRID)
PRR1-10.2196/44593