bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progestin Primed Ovarian Stimulation (PPOS) protocol yields lower euploidy rate in older patients undergoing IVF
Authors: Pai Angel Hsin-Yu , Sung Yen Ju , Li Chia-Jung , Lin Chieh- Yu , Chang Chia Lin 
Published: August 2023

Abstract
Background
To explore if exogenous progestin required for progestin primed ovarian stimulation (PPOS) protocol compromises the euploidy rate of patients who underwent preimplantation genetic testing cycles when compared to those who received the conventional gonadotropin-releasing hormone (GnRH) antagonist protocol.

Methods
This retrospective cohort study analyzed 128 preimplantation genetic testing for aneuploidy (PGT-A) cycles performed from January 2018 to December 2021 in a single university hospital-affiliated fertility center. Infertile women aged 27 to 45 years old requiring PGT-A underwent either PPOS protocol or GnRH-antagonist protocol with in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) for fertilization. Frozen embryo transfers were performed following each PGT-A cycle. Data regarding the two groups were analyzed using the Statistical Package for Social Sciences (SPSS) version 22.0 (SPSS Inc., Chicago, IL).

Results
Patients who underwent PPOS treatment had significantly reduced blastocyst formation rate and euploidy rate compared to those who received the GnRH antagonist protocol. Subgroup-analysis was performed by stratifying patients’ age into elder and young subgroups (elder: ≥ 38-year-old, young: < 38-year-old). In the elder sub-population, the blastocyst formation rate of the PPOS group was significantly lower than that of the GnRH-antagonist group (45.8 ± 6.1% vs. 59.9 ± 3.8%, p = 0.036). Moreover, the euploidy rate of the PPOS group was only about 20% of that of the GnRH-antagonist group (5.4% and 26.7%, p = 0.006). In contrast, no significant differences in blastocyst formation rate (63.5 ± 5.7% vs. 67.1 ± 3.2%, p = 0.45) or euploidy rate (30.1% vs. 38.5%, p = 0.221) were observed in the young sub-population. Secondary outcomes, which included implantation rate, biochemical pregnancy rate, clinical pregnancy rate, live birth rate, and miscarriage rate, were comparable between the two treatment groups, regardless of age.

Conclusion
When compared to the conventional GnRH-antagonist approach, PPOS protocol could potentially reduce the euploidy rate in aging IVF patients. However, due to the retrospective nature of this study, the results are to be interpreted with caution. Before the PPOS protocol is widely implemented, further studies exploring its efficacy in larger populations are needed to define the optimal patient selection suitable for this method.

Trial registration
Human Investigation and Ethical Committee of Chang Gung Medical Foundation (202200194B0).
Primary pulmonary hyalinizing clear cell carcinoma with vocal-cord squamous cell carcinoma: a case report with systematic review
Authors: Li Zhuo , Li Weihua , Xue Liyan 
Published: August 2023

Abstract
Background
Primary pulmonary hyalinizing clear cell carcinoma (HCCC) is a low-grade salivary gland-type carcinoma. Until now, 23 cases of pulmonary HCCC have been reported.

Case presentation
Here, we present a patient with primary pulmonary HCCC along with vocal-cord squamous cell carcinoma (SCC) revealed by biopsy examination. The patient underwent radiotherapy for vocal-cord SCC, followed by right upper lobectomy and lymph node dissection 10 months later. Histology revealed polygonal cells with eosinophilic or clear cytoplasm in the myxoid matrix together with hyaline degeneration. The tumor involved the whole layer of the segmental bronchus and regionally involved the alveolar tissue along with one intrapulmonary lymph node. Targeted RNA sequencing revealed Ewing Sarcoma Breakpoint Region 1 (EWSR1)- activating transcription factor 1 (ATF1) fusion. We analyzed the data on pulmonary malignant tumors between 2000 and 2019 in the Surveillance, Epidemiology, and End Results (SEER) database and reviewed all cases of pulmonary HCCC with EWSR1 fusion by searching PubMed. The results showed that head and neck (HN) adenoid cystic carcinoma (ACC) (47.89%) and HNSCC (22.54%) were the most common carcinomas occurring with pulmonary salivary gland-type malignant tumors. Screening of 24 cases of pulmonary HCCC with EWSR1 fusion revealed that five cases demonstrated lymph node metastases and only two had documented tumor recurrences. HCCC is rare and easily misdiagnosed as SCC, but the treatment regimen differs between pulmonary HCCC and SCC.

Conclusions
Hence, pulmonary tumors with clear cells must be diagnosed with caution. Next-generation sequencing (NGS) may be useful for diagnosis, especially in cases with a history of squamous cell carcinoma (SCC).
Multi-omics reveal the metabolic patterns in mouse cumulus cells during oocyte maturation
Authors: Chen Ming , Yang Weizheng , Guo Yueshuai , Hou Xiaojing , Zhu Shuai , Sun Hongzheng , Guo Xuejiang , Chen Minjian , Wang Qiang 
Published: August 2023

AbstractBi-directional communication between cumulus cells and the surrounded oocytes is important for the development and functions of both compartments. However, the metabolic framework in cumulus cells has not been systematically described. In the present study, cumulus cells from cumulus-oocyte complexes (COCs) at three key time points were isolated (arrested GV stage, post-hCG 0h; meiotic resumption GVBD stage, post-hCG 3h; and metaphase II stage, post-hCG 12h), and the temporal metabolomic and proteomic profiling were performed. Integrated multi-omics analysis reveals the global metabolic patterns in cumulus cells during mouse oocyte maturation. In particular, we found the active hyaluronic acid metabolism, steroid hormone synthesis, and prostaglandin E2 (PGE2) production in cumulus cells. Meanwhile, accompanying the oocyte maturation, a progressive increase in nucleotide and amino acid metabolism was detected in the surrounding cumulus cells. In sum, the data serve as a valuable resource for probing metabolism during terminal differentiation of ovarian granulosa cells, and provide the potential biomarkers for improving and predicting oocyte quality.
Neural stem cell-derived exosomes and regeneration: cell-free therapeutic strategies for traumatic brain injury
Authors: Zhong Lin , Wang Jingjing , Wang Peng , Liu Xiaoyin , Liu Peng , Cheng Xu , Cao Lujia , Wu Hongwei , Chen Jing , Zhou Liangxue 
Published: August 2023

AbstractRegenerative repair of the brain after traumatic brain injury (TBI) remains an extensive clinical challenge, inspiring intensified interest in therapeutic approaches to explore superior repair strategies. Exosome therapy is another research hotspot following stem cell alternative therapy. Prior research verified that exosomes produced by neural stem cells can participate in the physiological and pathological changes associated with TBI and have potential neuroregulatory and repair functions. In comparison with their parental stem cells, exosomes have superior stability and immune tolerance and lower tumorigenic risk. In addition, they can readily penetrate the blood‒brain barrier, which makes their treatment efficiency superior to that of transplanted stem cells. Exosomes secreted by neural stem cells present a promising strategy for the development of novel regenerative therapies. Their tissue regeneration and immunomodulatory potential have made them encouraging candidates for TBI repair. The present review addresses the challenges, applications and potential mechanisms of neural stem cell exosomes in regenerating damaged brains.
Mesenchymal stem cells shift the pro-inflammatory phenotype of neutrophils to ameliorate acute lung injury
Authors: Feng Bing , Feng Xudong , Yu Yingduo , Xu Haoying , Ye Qingqing , Hu Ruitian , Fang Xinru , Gao Feiqiong , Wu Jian , Pan Qiaoling , Yu Jiong , Lang Guanjing , Li Lanjuan , Cao Hongcui 
Published: August 2023

Abstract
Background
Mesenchymal stem cell (MSC) treatment plays a major role in the management of acute lung injury (ALI), and neutrophils are the initial line of defense against ALI. However, the effect of MSCs on neutrophils in ALI remains mostly unknown.

Methods
We investigated the characteristics of neutrophils in lung tissue of ALI mice induced by lipopolysaccharide after treatment with MSCs using single-cell RNA sequencing. Neutrophils separated from lung tissue in ALI were co-cultured with MSCs, and then samples were collected for reverse transcription-polymerase chain reaction and flow cytometry.

Results
During inflammation, six clusters of neutrophils were identified, annotated as activated, aged, and circulatory neutrophils. Activated neutrophils had higher chemotaxis, reactive oxygen species (ROS) production, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase scores than aged neutrophils. Circulatory neutrophils occurred mainly in healthy tissue and were characterized by higher expression of Cxcr2 and Sell. Activated neutrophils tended to exhibit higher expression of Cxcl10 and Cd47, and lower expression of Cd24a, while aged neutrophils expressed a lower level of Cd47 and higher level of Cd24a. MSC treatment shifted activated neutrophils toward an aged neutrophil phenotype by upregulating the expression of CD24, thereby inhibiting inflammation by reducing chemotaxis, ROS production, and NADPH oxidase.

Conclusion
We identified the immunosuppressive effects of MSCs on the subtype distribution of neutrophils and provided new insight into the therapeutic mechanism of MSC treatment in ALI.
Investigating the initial steps of auricin biosynthesis using synthetic biology
Authors: Csolleiova Dominika , Javorova Rachel , Novakova Renata , Feckova Lubomira , Matulova Maria , Opaterny Filip , Rezuchova Bronislava , Sevcikova Beatrica , Kormanec Jan 
Published: August 2023

AbstractStreptomyces lavendulae subsp. lavendulae CCM 3239 (formerly Streptomyces aureofaciens CCM 3239) contains a type II polyketide synthase (PKS) biosynthetic gene cluster (BGC) aur1 whose genes were highly similar to angucycline BGCs. However, its product auricin is structurally different from all known angucyclines. It contains a spiroketal pyranonaphthoquinone aglycone similar to griseusins and is modified with d-forosamine. Here, we describe the characterization of the initial steps in auricin biosynthesis using a synthetic-biology-based approach. We have created a plasmid system based on the strong kasOp* promoter, RBS and phage PhiBT1-based integration vector, where each gene in the artificial operon can be easily replaced by another gene using unique restriction sites surrounding each gene in the operon. The system was validated with the initial landomycin biosynthetic genes lanABCFDLE, leading to the production of rabelomycin after its integration into Streptomyces coelicolor M1146. However, the aur1DEFCGHA homologous genes from the auricin aur1 BGC failed to produce rabelomycin in this system. The cause of this failure was inactive aur1DE genes encoding ketosynthases α and β (KSα, KSβ). Their replacement with homologous aur2AB genes from the adjacent aur2 BGC resulted in rabelomycin production that was even higher after the insertion of two genes from the aur1 BGC, aur1L encoding 4-phosphopantetheinyl transferase (PPTase) and aur1M encoding malonyl-CoA:ACP transacylase (MCAT), suggesting that Aur1L PPTase is essential for the activation of the acyl carrier protein Aur1F. These results suggest an interesting communication of two BGCs, aur1 and aur2, in the biosynthesis of the initial structure of auricin aglycone.
Characterization of entomotoxic and nematotoxic genes from indigenous Bacillus thuringiensis strains and their biocontrol potential
Authors: Sujayanand G. K. , Pandey Sonika , Jagadeeswaran R. , Chandra Anup , Kumar Vaibhav , Dubey Sachin , Dubey Jyotirmay 
Published: August 2023

AbstractBackgroundThe gram pod borer (Helicoverpa armigeraHubner), Bihar hairy caterpillar (Spilosoma obliquaWalker), tobacco caterpillar (Spodoptera lituraFabricius) and root knot nematode (Meloidogyne incognita(Kofoid and White) Chitwood) are the major insect pest and nematodes infesting mungbean, urdbean and other legumes.Bacillus thuringiensis(Bt) is a promising biocontrol agent that helps in managing the above pest. DeployingBtbiopesticides instead of chemical pesticides in legume cultivation enhances the soil health substantially. The present study envisages morphological, molecular cum biochemical characterization, entomotoxicity and nematotoxicity potential of four indigenousBtstrains (Ak2.IIPR, F8.IIPR, F5.IIPR and F6.IIPR) along with HD1 (standard check from BGSC).ResultsThe SEM micrograph analysis of above fourBtstrains along with F1.IIPR revealed the presence of different combinations of insecticidal crystal proteins, viz. cuboidal crystal (CC), bipyramidal crystal (BC), spherical crystal (SC) and irregularly pointed crystal (IC). This was further confirmed by PCR amplification ofCry1,Cry2Aa,Cry2Ac,VIP3Aandchi36gene-specific primers and their phylogenetic analysis. F8.IIPR and Ak2.IIPR recorded the highest toxicity index against second-instarS. obliqualarvae (292.56 and 174.55),H. armigera(150.00 and 113.65) andS. litura(210.71 and 114.19) based on probit analysis. F5.IIPR (91.67%) and F8.IIPR (89.00%) recorded the highest juvenile mortality againstM. incognita, followed by Ak2.IIPR (70.33%). The protein profiling of supernatant of terrific broth inoculated with F8.IIPR indicated the presence of VIP3A and Cry1I.ConclusionThe present study concluded that F8.IIPR and Ak2.IIPR were potentialBtstrains isolated from Indo-Gangetic plains which is having both entomocidal and nematicidal properties. Further, these strains were deposited at national repository, ICAR-NBAIM with the following accession no.: NAIMCC-SB-065 and NAIMCC-SB-066, respectively. These strains can be formulated as microbial biopesticide.
Isothermal calorimetry calscreener in the metabolism gauge of human malignant neoplastic cells: a burgeoning nexus in cancer biochemical metrology and diagnostics
Authors: Bayode Michael Tosin , Alabi Mercy Adewumi , Ibisanmi Temitope Abraham , Okiti Ayomide Faith , Adebisi Olabisi Omowumi , Aro Philemon Olayemi , Awode Ayoola Emmanuel , Lawal Oyindamola Sukurat , Babatunde Olamide Joshua , Adenikinju Gladys Oluwafisayo 
Published: August 2023

Abstract
Background
At present, the function of isothermal microcalorimetry (IMC) calscreener in detecting alterations in the metabolic pathways of cancer cells remains unexplored. We disclosed the shortcomings of current screening methods and the need for precise and dependable instruments in the detection and informed treatment of cancer via the IMC in relation to the experimental procedures required to obtain accurate results.

Main body of abstract
We examined the intricate technical aspects of isothermal calorimetry. The application of the IMC calscreener in cancer research is then discussed in depth, including how it can be used to evaluate the efficacy of treatments, identify metabolic inhibitors, and assess metabolic rates. We also investigated the diagnostic potential of isothermal calorimetry, particularly for early cancer detection and tracing therapy efficacy.

Conclusions
General findings shed light on the present issues and potential approaches for isothermal calorimetry application in cancer research and diagnosis. We underline the potential for isothermal calorimetry to fundamentally alter how to understand and treat cancer, as well as the need for additional studies to maximize its application in clinical settings. This in turn offers a thorough and fascinating account of the emerging relationship between isothermal calorimetry and cancer biochemistry, as well as its potential to revolutionize cancer detection and therapy.
Challenges in differential diagnosis between autism spectrum disorder and psychotic disorders
Authors: Krysta K. 
Published: August 2023

AbstractData from literature show that people with Autism Spectrum Disorders (ASD) are more likely to develop a psychosis than the general population. They also have a higher chance of developing schizophrenia than neurotypical controls. In order to diagnose comorbid psychosis in ASD in an adult, first a full psychiatric examination is necessary to decide on the directions of possible further diagnostics and, if necessary, to recommend appropriate additional tests, e.g. psychological tests using professional scales and questionnaires for adults. In the case of diagnostic doubts, there are indications to refer the patient to more specialized centers. Although the two conditions are different, they share some common features, such as social withdrawal and communication disorders. This can lead to problems in the diagnosis of psychosis in people with autism. The basic symptoms of the mentioned diseases usually differ between the two disorders. For this reason, it’s important to try to get regular screenings and get care from the right professionals. A case report is presented of a patient, who received a diagnosis of ASD in the childhood, and later as an adult developed psychotic symptoms, which led to a change of diagnosis to schizophrenia.Disclosure of InterestNone Declared
Effectiviness of a crisis comunity-based program in primary mental health care
Authors: Lakis S. , Mansilla S. , Patrizzi B. , Teixidó C. , Vegué J. , Plaza A. 
Published: August 2023

IntroductionA crisis is defined as a disruption in equilibrium at the failure of own personal resources which results in important distress and functional impairment. Particularly after Covid-19 pandemic we have been attending an increased number of this kind of consultations in the mental health services.ObjectivesTo expose a new mental health program called PAIC (Ambulatory Intensive Community Program) started in March 2019 and addressed to people who are suffering a crises period, and to evaluate its effectiveness. The aim of this program is to reach prior stability, trying to avoid chronicity, clinical status perpetuation and sociofunctional impairment, as well as assessing suicidal risk and perform early prevention interventions.MethodsPAIC is proposed as an early, intensive, focused and psychotherapeutic intervention led by psychiatrist. Consist in an 8-week program of 30 to 60 minute weekly individual sessions. A total of a 205 patients were attended in PAIC during 2021 and 84,6% of the patients completed the program. Mixed-methods, clinical interviews and measurements using validated self-administrated questionnaires were used: CORE-OM pre/post treatment (“Clinical Outcomes in Routine Evaluation-Outcome Measure”), List of Threatening Experiences, LTE and Clinical Global Impression (CGI-I). Changes in CORE-OM Scale were analysed using t-student test and a descriptive analysis was used for CGI-results.ResultsCORE-OM (“Clinical Outcomes in Routine Evaluation-Outcome Measure”) showed improvement in all of four dimensions: subjective well-being (TW), problems/symptoms (PT), general function (TF) and risk (TR).Perception of clinical improving measured by CGI was 81,6%. There were no cases of clinical worsening.ConclusionsWe conclude that intensive and early programs are effective reducing the intensity of symptoms and the level of disability in people who are experiencing a psychological crisis. Also, it supports primary health care as well as helps to avoid saturation of specialized system.Disclosure of InterestNone Declared
Thyroid disorders in psychiatric patients: a descriptive study in a psychiatric hospital
Authors: López U. , Morado San Segundo L. , González Navarro C. , Alonso Salas I. , López Fariña A. , Bilbao Idarraga A. , Samsó Martínez B. , López Brokate R. F. , Garnica de Cos E. M. , Ruiz de Azua Aspizua T. , Ortega Pozas U. 
Published: August 2023

IntroductionThyroid disorders can present with psychiatric symptons similar to depression, and, at the same time, certain treatments, like litio, can cause changes in thyroid function. Given, therefore, the importance for the treatment and care of patients, the study of thyroid function is one of the parametres that should be requested in patients with psychiatric pathology.ObjectivesTo study the frequency of thyroid disorders in patients who where admitted to a psychiatric short stay unit.MethodsRetrospective descriptive observational study is carried out in the acute stay unit of a psychiatric hospital. As a sample, all patients admitted to the unit over a period of three months. During admission, their sociodemographic data, the treatment they receive and their diagnosis are recorded. Secondly, blood test are performed whith differents parameters, including TSH values.ResultsIn the total sample of 172 patients, 8 of them have TSH abnormalities. 7 of them, all women, present hypothyroidism values.A single male patient presented values of hyperthryroidism.ConclusionsAccording to the present study, 4,6% of the patients present alterations at the TSH at admission, although except in one case, the values were not markedly altered.The thyroid study at admission allows detecting cases of altered TSH that are amenable to treatment and monitoring.Disclosure of InterestNone Declared
Posttraumatic orientation to bodily signals: The engraving of trauma in bodily perceptions
Authors: Tsur N. , Solomon Z. 
Published: August 2023

IntroductionTheoretical perspectives emphasize that trauma and complex/posttraumatic stress disorder (C/PTSD) may interrupt with the perception of normal day-to-day bodily sensations, such as hunger, temperature and pain. Yet, a coherent conceptual synthesis of such processes is still lacking.ObjectivesThis presentation portrayes two studies that provide empirical grounding for the conceptualization of ‘Posttraumatic Orientation to Bodily Signals’ (posttraumatic-OBS); an umbrella term reflecting the tendency to interpret bodily signals as catastrophic and frightful following trauma.MethodsTwo studies assessing exposure to trauma, C/PTSD, and OBD (Pain catastrophizing scale, PCS; body vigilance scale, BVS; Anxiety sensitivity index-physical), were conducted to test the hypothesized association between exposure to trauma and posttraumatic-OBD, as explained by C/PTSD.ResultsStudy 1 included 59 ex-prisoners of war and 44 controls along three time-points, revealing that exposure to trauma was associated with a more catastrophic OBS (t = 2.73, p = .008; Cohen’s d = .57), which was mediated by longitudinal hyperarousal PTSD symptoms (indirect effect = .04 [.009, .11]). Additionally, a long-term chronic trajectory of PTSD was implicated in a more catastrophic OBS (F (2102)=6.91, p = .046).Study 2 included 194 dyads of mothers and their young adult daughter. Dyadic path analyses demonstrated that OBD was associated with exposure to trauma, through the mediation of CPTSD among mothers (indirect effects between 0.13–0.28; p > 0.021) and daughters (indirect effects between 0.21–0.11; p > 0.032). Mothers’ OBD was associated with daughters’ OBD (effects between 0.19-0.27; p < 0.016). Daughters’ OBD was serially associated with mothers’ trauma exposure through mothers’ CPTSD and mothers’ OBD, (indirect effect = 0.064; p = 0.023). The findings demonstrate that trauma is often implicated in posttraumatic-OBD, which is mediated by C/PTSD, and that these processes may be intergenerationally transmitted.ConclusionsThe findings lay the foundation for the conceptualization of posttraumatic-OBD. The implications of the unified encapsulation of posttraumatic-OBD as an umbrella term reflecting subjective perception of bodily sensations for future research and practice will be presented.Disclosure of InterestNone Declared