bluuebunny/crossref_metadata_2025_split huggingface dataset:
Late anastomotic complication after laparoscopic surgery for clinical stage I low rectal cancers located within 5 cm of the anal verge: Sub‐analysis of the ultimate trial
Authors: Shimomura Manabu , Yoshimitsu Masanori , Tsukada Yuichiro , Ohdan Hideki , Watanabe Jun , Fukunaga Yosuke , Hirano Yasumitsu , Sakamoto Kazuhiro , Hamamoto Hiroki , Horie Hisanaga , Matsuhashi Nobuhisa , Kuriu Yoshiaki , Nagai Shuntaro , Hamada Madoka , Yoshioka Shinichi , Ohnuma Shinobu , Hayama Tamuro , Otsuka Koki , Inoue Yusuke , Ueda Kazuki , Toiyama Yuji , Maruyama Satoshi , Yamaguchi Shigeki , Tanaka Keitaro , Suzuki Motoko , Misumi Toshihiro , Naitoh Takeshi , Watanabe Masahiko , Ito Masaaki ,   Ultimate Trial Group
Published: January 2025

AbstractBackgroundAlthough extensive research has been conducted on early anastomotic leakage (AL) after sphincter‐sparing surgery, the status of late anastomotic complications (post‐30 days) has received limited attention. These late complications significantly affect a patient's quality of life and often lead to permanent stoma creation.MethodsThis study conducted a sub‐analysis of a phase II trial assessing the outcomes of laparoscopic surgery for cStage I lower rectal adenocarcinoma (the ultimate trial). This study included 278 patients who underwent intestinal anastomosis and investigated the frequency, timing, and risk factors of late anastomotic complications (stenosis, fistula, and intestinal prolapse).ResultsAnastomotic stenosis occurred in 27 patients (9.7%), and the median time of occurrence was 274 days (range, 70–1226 days). Only early AL (p = 0.004) was identified as an independent risk factor. A late anastomotic fistula was observed in five patients (1.8%), and 18 patients (6.4%) requiring permanent stomas. A short distance from the lower tumor margin to the anal verge (AV) (p = 0.004) and the presence of stenosis or fistula (p < 0.0001) were independent risk factors.Intestinal prolapse occurred in eight cases (3%), with a median occurrence of 221 days (range, 122–725 days). Intersphincteric resection (ISR) (p = 0.02) and splenic flexure takedown (p < 0.0001) were independent risk factors.ConclusionAnastomotic stenosis and late fistula formation frequently emerge as secondary consequences of early AL and represent significant complications linked to permanent stoma creation, often proving resistant to treatment. Intestinal prolapse is a characteristic anastomotic complication of ISR that can be caused by excessive intestinal mobilization.
Breaking the Bone Marrow Barrier: Peripheral Blood as a Gateway to Measurable Residual Disease Detection in Acute Myelogenous Leukemia
Authors: Butler John T. , Yashar William M. , Swords Ronan 
Published: January 2025

ABSTRACTAcute myeloid leukemia (AML) is a genetically heterogeneous disease with high rates of relapse after initial treatment. Identifying measurable residual disease (MRD) following initial therapy is essential to assess response, predict patient outcomes, and identify those in need of additional intervention. Currently, MRD analysis relies on invasive, serial bone marrow (BM) biopsies, which complicate sample availability and processing time and negatively impact patient experience. Additionally, finding a positive result can generate more questions than answers, causing anxiety for both the patient and the provider. Peripheral blood (PB) evaluation has shown promise in detecting MRD and is now recommended by the European Leukemia Net for AML for certain genetic abnormalities. PB‐based sampling allows for more frequent testing intervals and better temporal resolution of malignant expansion while sparing patients additional invasive procedures. In this review, we will discuss the current state of PB testing for MRD evaluation with a focus on next‐generation sequencing methodologies that are capable of MRD detection across AML subtypes.
Secondary Metabolites from Tripterygium wilfordii Hook. f.‐Associated Endophytes: Producing Microbes, Structures, and Bioactivities
Authors: Chen Cuili , Lu Shan , Zou Yanmin , Mao Di , Kakeya Hideaki 
Published: January 2025

AbstractEndophytes have been recognized as important sources for discovering novel bioactive natural products with diverse structures and biological activities. This review focuses on the endophytes of Tripterygium wilfordii Hook. f. (T. wilfordii), a famous traditional medicinal plant, and summarizes the producing microbes, chemical diversities, and bioactivities of the metabolites discovered from the T. wilfordii ‐associated endophytes over the past thirty years (1995–2024). One hundred and one metabolites together with their bioactivities from twelve endophytes are systematically reviewed. A comprehensive comparison of the 101 metabolites with those isolated from T. wilfordii reveals that the T. wilfordii‐associated endophytes produce structurally distinct metabolites with diverse biological activities. To the best of our knowledge, this is the first holistic overview of the T. wilfordii‐associated endophytes and their secondary metabolites.
Anaesthetic management of a large multi‐nodular goitre with dynamic bilateral carotid artery compression
Authors: Milder D. A. , Zaklama R. , Wang K. , Harrison J. , Sandler G. 
Published: January 2025

SummaryDynamic carotid compression arising from a change in patient position is a rare complication of goitre, with the potential for cerebral ischaemia and infarction. In this report, a 37‐year‐old woman presented with a multi‐nodular goitre with clinical features concerning for transient cerebral ischaemia. The anaesthetic management and neurological monitoring used to mitigate the risk of cerebral ischaemia during thyroidectomy is described and the literature surrounding this rare complication is explored.
Irrigation Expansion and Rural Transformation in China: Evidence of Provincial Data in 1978–2018
Authors: Shi Pengfei , Huang Jikun 
Published: January 2025

ABSTRACTThe role of irrigation expansion in facilitating rural transformation has been widely documented, yet quantitative studies remain rather limited. Based on the statistical data from 27 provinces in China, this paper analyses the correlation between irrigation expansion and rural transformation over the period of 1978–2018. The econometric results indicate a positive relationship between irrigation expansion and high‐value agriculture. However, from a dynamic perspective, for each percentage point increase in the share of cultivated land with irrigation, the share of high‐value agriculture decreases by 0.09 percentage points in the early stage, but increases by 0.07 and 0.12 percentage points in the subsequent middle and later stages, respectively. Likewise, the relationship between irrigation expansion and non‐farm employment of rural labourers is not always negative. Specifically, an additional one percentage point increase in the share of cultivated land with irrigation is associated with a 0.09 percentage points decline in the share of rural labour non‐farm employment in the early stage. This reduction moderates to a 0.05 percentage points in the middle stage, but there is a 0.08 percentage points increase in the later stage. This paper further concludes with several implications for policymakers and academic research.
Antioxidant activity, phytochemical composition, and antitumor capacity of Amazonian fruits taperebá (Spondias mombin) and murici (Byrsonima crassifolia)
Authors: Lima Thuane Passos Barbosa , Coimbra Pedro Paulo Saldanha , da Silva Antonio Ananda , Pereira Henrique Marcelo Gualberto , da Silva Giovana Ramalho Patrizi , da Veiga‐Junior Valdir Florêncio , Silva Otniel Freitas , Felzenszwalb Israel , Araujo‐Lima Carlos Fernando , Teodoro Anderson Junger 
Published: January 2025

AbstractThe Amazon rainforest is renowned for its biodiversity and as a reservoir of edible and medicinal plants. The phytochemicals in murici and taperebá fruits serve as natural antioxidants, contributing to cultural preservation, ecosystem protection, and economic opportunities. However, limited scientific research on their composition and health benefits hinders their recognition as functional foods. This study aimed to evaluate the antioxidant activity, carotenoid content, phenolic compounds, and antitumor effects of murici and taperebá fruit pulps. Four antioxidant tests (2,2‐Diphenyl‐1‐picrylhydrazylradical scavenging activity, 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) method, 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) method, oxygen radical absorbance capacity) were conducted, and total phenolics were quantified (Folin‐Ciocalteu). Phenolics were identified using UHPLC‐HRMS, and carotenoids by high‐performance liquid chromatography (HPLC). The impact on breast cancer cell viability (MCF‐7, MDA‐MB‐231) was assessed via water‐soluble tetrazolium (WST) assay. Both fruits showed high antioxidant activity and phenolic content, with murici leading. HPLC revealed five carotenoids per fruit, with taperebá showing higher concentrations. UHPLC‐HRMS identified 23 phenolic compounds: 16 in murici aqueous extract, 18 in murici ethanolic extract, and 15 in each taperebá extract. WST assay demonstrated that both fruits exerted a significant impact on breast cancer cells, reducing their viability in a dose‐dependent manner. These findings underscore the potential of murici and taperebá as sources of phytochemical antioxidants and antiproliferative agents with promising health applications.
Triple exponentially weighted moving average controller optimization using deep reinforcement learning in semiconductor manufacturing process
Authors: Pan Tianhong , Jin Biao , Ma Zhu , Fang Liandi 
Published: January 2025

AbstractThe triple exponentially weighted moving average (TriEWMA) controller integrates three distinct EWMAs to mitigate process dynamics and disturbances, enhancing system stability in the semiconductor manufacturing process. However, the performance of TriEWMA is highly dependent on its weight parameters, which are challenging to optimize manually. This work develops a composite strategy utilizing deep reinforcement learning to autonomously optimize these weights. Specifically, the weight adjustment process is modeled as a Markov decision process. Furthermore, the deep deterministic policy gradient (DDPG) algorithm is employed for optimization. The DDPG agent acts as an auxiliary controller to generate the ideal weights of TriEWMA, while the TriEWMA acts as the primary controller to generate appropriate recipes after obtaining the optimal combination of weights. Finally, the proposed algorithm demonstrates superior performance in handling process dynamics and disturbance rejection, as evidence in simulations of a chemical mechanical polishing process.
Development of a Novel Synchronous Spectrofluorimetric Method Coupled With Chemometric Modelling for Ultrasensitive Analysis of Tenofovir and Dolutegravir With Implication for Pharmacokinetic Studies
Authors: Alnemari Reem M. , Alaqel Saleh I. , Abduljabbar Maram H. , Almalki Atiah H. , Serag Ahmed 
Published: January 2025

ABSTRACTThis study introduces a novel synchronous spectrofluorimetry coupled with chemometric tools for the determination of tenofovir and dolutegravir antiretroviral drugs. Utilizing partial least squares regression (PLS) fine‐tuned by genetic algorithm as variable selection tool, the developed models demonstrate greater sensitivity, cost‐effectiveness, and reduced environmental impact compared to traditional HPLC methods. The model's validation was further confirmed using external validation in addition to QC samples as per ICH M10 guidelines, which yielded high accuracy ranged between 94.78% and 102.48% for tenofovir and 94.56% and 103.31% for dolutegravir, respectively. Additionally, the %CV values for within‐run precision were below 4%, while those for between‐run precision stayed under 6%, for tenofovir and dolutegravir, respectively. Hence, the developed models were adeptly applied to pharmacokinetic studies in a rat model, revealing slight changes in the tenofovir and dolutegravir plasma concentration profiles and half‐lives when administered in combination, highlighting possible drug interactions. Greenness analysis of this spectrofluorimetric approach using the AGREE tool further substantiates its advantages over conventional chromatographic techniques. Hence, this simple, rapid, and environmentally friendly analytical methodology presents a promising alternative for therapeutic drug monitoring and pharmacokinetic studies of tenofovir and dolutegravir antiretroviral drugs.
Building a teaching method for “clinical molecular biology testing technology” guided by the cultivation of thinking skills
Authors: Deng Xin , Ma Nuoya , Hu Xinwen , Li Yong , He Junyu , Zou Guoying 
Published: January 2025

AbstractThe knowledge of “clinical molecular biology testing technology” is complex, conceptual expressions are abstract and difficult to understand, and the student's interest in learning is low. This study aimed to evaluate the effectiveness of a cyclic teaching method based on case analysis combined with an exploratory teaching method using mind mapping as an assignment. Students from the 2019 cohort of medical laboratory technology at Hunan University of Chinese Medicine served as the control group and received conventional lecture‐based teaching methods. Students from the 2021 cohort served as the test group and were undergoing teaching methods that focused on developing critical thinking skills. Course regular grades, exam scores, teaching evaluations, and questionnaires were used to evaluate the teaching effect. The test group had higher scores than the control group. It is also better in terms of memorization ability and comprehensive analytical ability. However, there were no differences between the two groups in terms of understanding and application ability. The test group showed higher satisfaction in improving knowledge mastery, cultivating learning interest, enhancing logical thinking ability, and utilizing post‐class assignments in the form of mind mapping for better knowledge consolidation and expansion than did the control group. The survey results from the test group indicate that a teaching method focused on developing thinking skills has significant effects on stimulating learning interest, improving cognitive ability, and helping to address clinical issues. Combining the cyclic teaching method based on case analysis with the exploratory teaching method using mind mapping as assignments can enhance learning motivation, facilitate knowledge acquisition, and is suitable for teaching in the discipline of “clinical molecular biology testing technology”.
How Climate Change Anxiety Enhances Sustainable Trust and Consumption Behavior: Evidence From Vietnam and Italy
Authors: Nguyen Khoi Minh , Nguyen Ngan Thanh , Ngo Tra Linh , Le Thuy Thu 
Published: January 2025

ABSTRACTThis study explores the intricate associations between climate change anxiety, sustainable trust and three aspects of sustainable consumption behaviors (quality of life, care for environment and care for future generations) among consumers in Vietnam and Italy, with a focus on the moderating role of nature connectedness and hyperopia psychology. Employing an exploratory sequential mixed‐methods approach that integrates both qualitative and quantitative techniques, the study initially conducted 20 in‐depth interviews to gain insights into the interplay between climate change‐related factors, trust, and sustainable consumer behaviors, which helped to formulate the quantitative research framework. Subsequently, a quantitative approach was utilized, gathering 681 online surveys from Vietnamese and Italian consumers, with the data assessed through structural equation modeling using SmartPLS software. This study is the first to reveal crucial theoretical insights into how climate change anxiety and sustainable trust, affected by climate change risk perception and climate change knowledge, impact three aspects of sustainable consumption behaviors: quality of life, care for the environment, and care for future generations. These effects are positively moderated by hyperopia and nature connectedness, providing profound understanding of consumer behavior between the two regions within sustainable consumption amid the urgent global climate change crisis. Scholars benefit from an enriched understanding of consumer behaviors amidst climate change anxiety, while policymakers, businesses, and advocates gain actionable insights to drive effective climate mitigation and adaptation strategies, which contributes to advancing the United Nations' Sustainable Development Goals.
Identification of significant single‐nucleotide polymorphisms associated with breast cancer recurrence and metastasis using GWAS
Authors: Sun Shujuan , Yin Sha , Huang Jie , Zhou Dongdong , Tan Qiaorui , Man Xiaochu , Wang Wen , Zhang Jiale , Li Huihui 
Published: January 2025

AbstractBackgroundIdentification of risk genes and loci associated with the recurrence and metastasis of breast cancer (BC) is of utmost importance. Genome‐wide association studies (GWASs) represent valuable tools for identifying the disease risk associated with a given single‐nucleotide polymorphism (SNP); they offer significant insights into the disease progression mechanism by analyzing SNP information of the entire genome. Though GWAS has already identified several genetic susceptibility SNPs for BC, their significance in the recurrence and metastasis of this cancer remains unclear. Here, we used a GWAS approach to identify SNPs specifically associated with the risk of BC recurrence and metastasis.MethodsThis study adopted a two‐stage GWAS approach. In the first stage, 97 pairs of BC patients with or without recurrence and metastasis, treated at the Shandong Cancer Hospital and Institute from November 2013 to April 2014, were identified using propensity score matching. DNA extracted from the patient peripheral blood was then subjected to Illumina ASA chip analysis for genome‐wide SNP detection. In the second stage, the findings were verified in a validation set of 854 BC patients recruited at the same hospital from May 2014 to June 2015. SNP genotyping was performed using time‐of‐flight mass spectrometry. The SNP loci and their corresponding genes and pathways were analyzed using the DAVID (https://david.ncifcrf.gov/) online enrichment analysis tool.ResultsBased on the GWAS results, 191 SNP‐related genes significantly associated with BC recurrence and metastasis were identified as expression quantitivative trait loci (p < 0.001). Functional and pathway enrichment analyses subsequently revealed the potential involvement of glutamatergic synaptic transmission, calcium signaling, and insulin secretion pathways in BC recurrence and metastasis. Based on genotype correlation and database expression levels, rs10108514, rs12920540, rs4273077, and rs4730155 were found to be significantly associated with the risk of BC recurrence and metastasis.ConclusionOur study suggests that the SNPs rs10108514, rs12920540, rs4273077, and rs4730155 are correlated with the risk of BC recurrence and metastasis, potentially by being implicated in glutamatergic synaptic transmission, calcium signaling, and insulin secretion pathways.
Fronto‐Orbital Osteoblastoma With Unilateral Exophthalmos: A Rare Presentation and Review of Diagnostic Challenges
Authors: Khorraminejad‐Shirazi Mohammadhossein , Nabavizadeh Ali , Bojnoordi Anita , Firouzabadi Dena , Rakhsha Abbas , Hosseini Seyed Ali , Dehghanian Amirreza 
Published: January 2025

ABSTRACTOsteoblastoma is an uncommon benign bone tumor rarely involving the craniofacial skeleton. Manifestations in the fronto‐orbital region are exceptionally rare. A 19‐year‐old man presented with persistent headache, nausea, vomiting, right eye pain, and longstanding right exophthalmos. Imaging revealed a heterogeneous enhancing lesion located in the right anterior cranial fossa‐orbital apex junction causing pressure on the orbital roof. Differential diagnoses included fibrous dysplasia and meningioma. The tumor was resected via frontal craniotomy. Definitive diagnosis of osteoblastoma was achieved postoperatively through histopathological examination and IHC studies. Craniofacial osteoblastomas, especially in the fronto‐orbital region, are exceptionally rare and may present radiological features similar to other bone pathologies. Accurate diagnosis hinges on histopathological evaluation. A multidisciplinary approach is pivotal for successful diagnosis and treatment of such challenging cases.
Impact of Sotorasib, a KRAS G12C Inhibitor, on the Pharmacokinetics and Therapeutic Window of Digoxin, a P‐Glycoprotein Substrate
Authors: Cardona Panli , Houk Brett 
Published: January 2025

AbstractSotorasib is a small‐molecule Kirsten rat sarcoma viral oncogene homolog (KRAS) G12C inhibitor indicated for the treatment of KRAS G12C‐driven cancers. KRAS G12C is a common mutation in solid tumors, including non‐small cell lung cancer. In vitro studies suggested that sotorasib is a weak inhibitor of P‐glycoprotein transporter. Digoxin is a known substrate for P‐glycoprotein. The primary objective of this study was to assess the impact of sotorasib on digoxin pharmacokinetics in healthy subjects. This Phase 1, open‐label, fixed‐sequence study enrolled 14 healthy subjects. Each subject received 0.5 mg of digoxin on Day 1 and 960 mg of sotorasib followed by 0.5 mg of digoxin on Day 7. Blood samples for digoxin pharmacokinetics were collected before dosing and up to 144 hours after the digoxin dose. Digoxin median time to maximum observed plasma concentration and mean terminal half‐life were similar following coadministration of digoxin with sotorasib compared with those of digoxin alone. Geometric mean digoxin area under the concentration‐time curve from time 0 extrapolated to infinity following coadministration of digoxin with sotorasib (40.3 h•ng/mL) was similar to that of digoxin alone (33.2 h•ng/mL). Geometric mean digoxin maximum observed plasma concentration following coadministration of digoxin with sotorasib (3.64 ng/mL) was higher compared with that of digoxin alone (1.90 ng/mL). Coadministration of digoxin and sotorasib did not impact sotorasib exposure. Single doses of 0.5 mg of digoxin were safe and well tolerated when administered alone or coadministered with 960 mg of sotorasib. Coadministration of digoxin with a single dose of sotorasib increased digoxin area under the concentration‐time curve from time 0 extrapolated to infinity and maximum observed plasma concentration by factors of 1.21 and 1.91, respectively, compared with digoxin alone.
Analysis of Sheet Thickness and Gate Voltage Dependence of Electrical Characteristics for Nanosheet MOSFETs
Authors: Hosoda Kosei , Hiroki Akira 
Published: January 2025

ABSTRACTIn this paper, we have analyzed sheet thickness and gate voltage dependence of electrical characteristics for nanosheet MOSFETs using a device simulation that takes quantum confinement effects into account. Since the sheet thickness of nanosheet MOSFETs decreases to several nanometers, the simulated on‐current shows significant reduction compared to the on‐current required in the device design due to the quantum confinement effect. We analyze the relative difference between the on‐current required in the device design and the simulated on‐current and propose a design guideline using the relative difference. In order to suppress the relative difference of the on‐currents within 3%, the minimum sizes of the sheet thickness are 3.4, 4.5, and 5.5 nm at supply voltages of 0.70, 0.60, and 0.50 V, respectively.