bluuebunny/crossref_metadata_2025_split huggingface dataset:
GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
Authors: Zou Xiuqun , Ma Li , Zhang Yihong , Zhang Qun , Xu Chu , Zhang Dan , Chu Yimin , Zhang Jie , Li Mengying , Zhang Hui , Wang Jiamin , Peng Chicheng , Wei Gang , Wu Yingjie , Hou Zhaoyuan , Jia Hao 
Published: June 2023

AbstractThe transcriptional repressor Snail induces EMT during embryonic development and tumor metastasis. Growing evidence indicates that Snail functions as a trans-activator to induce gene expression; however, the underlying mechanism remains elusive. Here, we report that Snail cooperates with GATA zinc finger protein p66β to transactivate genes in breast cancer cells. Biologically, depletion of p66β reduces cell migration and lung metastasis in BALB/c mice. Mechanistically, Snail interacts with p66β and cooperatively induces gene transcription. Notably, a group of genes induced by Snail harbor conserved G-rich cis-elements (5′-GGGAGG-3′, designated as G-box) in their proximal promoter regions. Snail directly binds to G-box via its zinc fingers and transactivates the G-box-containing promoters. p66β enhances Snail binding affinity to G-box, whereas depletion of p66β results in a decreased binding affinity of Snail to the endogenous promoters and concomitantly reduces the transcription of Snail-induced genes. Taken together, these data demonstrated that p66β is critical for Snail-mediated cell migration by acting as a co-activator of Snail to induce genes containing G-box elements in the promoters.
Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma
Authors: Liu Jian , Guo Yongjian , Zhang Ruitian , Xu Ye , Luo Chengju , Wang Rui , Xu Shu , Wei Libin 
Published: June 2023

AbstractHepatocellular carcinoma (HCC) is a malignant tumor, frequently causing both intrahepatic and extrahepatic metastases. The overall prognosis of patients with metastatic HCC is poor. Recently, single-cell (sc) polarity is proved to be an innate feature of some tumor cells in liquid phase, and directly involved in the cell adhesion to blood vessel and tumor metastasis. Here, we characterize the maintained sc polarity of HCC cells in a suspension culture, and investigate its roles and regulatory mechanisms during metastasis. We demonstrate that transient receptor potential vanilloid 4 (TRPV4) is a promoting regulator of sc polarity via activating Ca2+-dependent AMPK/MLC/ERM pathway. This attenuates the adhesion of metastatic HCC cells to vascular endothelial cells. The reduction of cancer metastases can result from TRPV4 inhibition, which not only impacts the migration and invasion of tumor cells, but also prevents the adhesion to vascular endothelial cells. Additionally, we discover a brand-new TRPV4 inhibitor called GL-V9 that modifies the degree of sc polarization and significantly decreases the metastatic capacity of HCC cells. Taken together, our data shows that TRPV4 and calcium signal are significant sc polarity regulators in metastatic HCC, and that the pharmacological intervention that results in HCC cells becoming depolarized suggests a promising treatment for cancer metastasis.
The evolution of dam induced river fragmentation in the United States
Authors: Spinti Rachel A. , Condon Laura E. , Zhang Jun 
Published: June 2023

AbstractIt is established that dams decrease river connectivity; however, previous global scale studies of river fragmentation focused on a small subset of the largest dams. In the United States, mid-sized dams, which are too small for global databases, account for 96% of major anthropogenic structures and 48% of reservoir storage. We conduct a national evaluation of the evolution of anthropogenic river bifurcation over time that includes more than 50,000 nationally inventoried dams. Mid-sized dams account for 73% of anthropogenically created stream fragments nationally. They also contribute disproportionately to short fragments (less than 10 km), which is particularly troubling for aquatic habitats. Here we show that dam construction has essentially reversed natural fragmentation patterns in the United States. Prior to human development, smaller river fragments and less connected networks occurred in arid basins while today we show that humid basins are the most fragmented due to human structures.
Electrical detection of spin pumping in van der Waals ferromagnetic Cr2Ge2Te6 with low magnetic damping
Authors: Xu Hongjun , Jia Ke , Huang Yuan , Meng Fanqi , Zhang Qinghua , Zhang Yu , Cheng Chen , Lan Guibin , Dong Jing , Wei Jinwu , Feng Jiafeng , He Congli , Yuan Zhe , Zhu Mingliang , He Wenqing , Wan Caihua , Wei Hongxiang , Wang Shouguo , Shao Qiming , Gu Lin , Coey Michael , Shi Youguo , Zhang Guangyu , Han Xiufeng , Yu Guoqiang 
Published: June 2023

AbstractThe discovery of magnetic order in atomically-thin van der Waals materials has strengthened the alliance between spintronics and two-dimensional materials. An important use of magnetic two-dimensional materials in spintronic devices, which has not yet been demonstrated, would be for coherent spin injection via the spin-pumping effect. Here, we report spin pumping from Cr2Ge2Te6 into Pt or W and detection of the spin current by inverse spin Hall effect. The magnetization dynamics of the hybrid Cr2Ge2Te6/Pt system are measured, and a magnetic damping constant of ~ 4–10 × 10−4 is obtained for thick Cr2Ge2Te6 flakes, a record low for ferromagnetic van der Waals materials. Moreover, a high interface spin transmission efficiency (a spin mixing conductance of 2.4 × 1019/m2) is directly extracted, which is instrumental in delivering spin-related quantities such as spin angular momentum and spin-orbit torque across an interface of the van der Waals system. The low magnetic damping that promotes efficient spin current generation together with high interfacial spin transmission efficiency suggests promising applications for integrating Cr2Ge2Te6 into low-temperature two-dimensional spintronic devices as the source of coherent spin or magnon current.
Plant traits poorly predict winner and loser shrub species in a warming tundra biome
Authors: García Criado Mariana , Myers-Smith Isla H. , Bjorkman Anne D. , Normand Signe , Blach-Overgaard Anne , Thomas Haydn J. D. , Eskelinen Anu , Happonen Konsta , Alatalo Juha M. , Anadon-Rosell Alba , Aubin Isabelle , te Beest Mariska , Betway-May Katlyn R. , Blok Daan , Buras Allan , Cerabolini Bruno E. L. , Christie Katherine , Cornelissen J. Hans C. , Forbes Bruce C. , Frei Esther R. , Grogan Paul , Hermanutz Luise , Hollister Robert D. , Hudson James , Iturrate-Garcia Maitane , Kaarlejärvi Elina , Kleyer Michael , Lamarque Laurent J. , Lembrechts Jonas J. , Lévesque Esther , Luoto Miska , Macek Petr , May Jeremy L. , Prevéy Janet S. , Schaepman-Strub Gabriela , Sheremetiev Serge N. , Siegwart Collier Laura , Soudzilovskaia Nadejda A. , Trant Andrew , Venn Susanna E. , Virkkala Anna-Maria 
Published: June 2023

AbstractClimate change is leading to species redistributions. In the tundra biome, shrubs are generally expanding, but not all tundra shrub species will benefit from warming. Winner and loser species, and the characteristics that may determine success or failure, have not yet been fully identified. Here, we investigate whether past abundance changes, current range sizes and projected range shifts derived from species distribution models are related to plant trait values and intraspecific trait variation. We combined 17,921 trait records with observed past and modelled future distributions from 62 tundra shrub species across three continents. We found that species with greater variation in seed mass and specific leaf area had larger projected range shifts, and projected winner species had greater seed mass values. However, trait values and variation were not consistently related to current and projected ranges, nor to past abundance change. Overall, our findings indicate that abundance change and range shifts will not lead to directional modifications in shrub trait composition, since winner and loser species share relatively similar trait spaces.
Acute stress induces long-term metabolic, functional, and structural remodeling of the heart
Authors: Yoganathan Thulaciga , Perez-Liva Mailyn , Balvay Daniel , Le Gall Morgane , Lallemand Alice , Certain Anais , Autret Gwennhael , Mokrani Yasmine , Guillonneau François , Bruce Johanna , Nguyen Vincent , Gencer Umit , Schmitt Alain , Lager Franck , Guilbert Thomas , Bruneval Patrick , Vilar Jose , Maissa Nawal , Mousseaux Elie , Viel Thomas , Renault Gilles , Kachenoura Nadjia , Tavitian Bertrand 
Published: June 2023

AbstractTakotsubo cardiomyopathy is a stress-induced cardiovascular disease with symptoms comparable to those of an acute coronary syndrome but without coronary obstruction. Takotsubo was initially considered spontaneously reversible, but epidemiological studies revealed significant long-term morbidity and mortality, the reason for which is unknown. Here, we show in a female rodent model that a single pharmacological challenge creates a stress-induced cardiomyopathy similar to Takotsubo. The acute response involves changes in blood and tissue biomarkers and in cardiac in vivo imaging acquired with ultrasound, magnetic resonance and positron emission tomography. Longitudinal follow up using in vivo imaging, histochemistry, protein and proteomics analyses evidences a continued metabolic reprogramming of the heart towards metabolic malfunction, eventually leading to irreversible damage in cardiac function and structure. The results combat the supposed reversibility of Takotsubo, point to dysregulation of glucose metabolic pathways as a main cause of long-term cardiac disease and support early therapeutic management of Takotsubo.
Detection of a pair density wave state in UTe2
Authors: Gu Qiangqiang , Carroll Joseph P. , Wang Shuqiu , Ran Sheng , Broyles Christopher , Siddiquee Hasan , Butch Nicholas P. , Saha Shanta R. , Paglione Johnpierre , Davis J. C. Séamus , Liu Xiaolong 
Published: June 2023

AbstractSpin-triplet topological superconductors should exhibit many unprecedented electronic properties, including fractionalized electronic states relevant to quantum information processing. Although UTe2 may embody such bulk topological superconductivity1–11, its superconductive order parameter Δ(k) remains unknown12. Many diverse forms for Δ(k) are physically possible12 in such heavy fermion materials13. Moreover, intertwined14,15 density waves of spin (SDW), charge (CDW) and pair (PDW) may interpose, with the latter exhibiting spatially modulating14,15 superconductive order parameter Δ(r), electron-pair density16–19 and pairing energy gap17,20–23. Hence, the newly discovered CDW state24 in UTe2 motivates the prospect that a PDW state may exist in this material24,25. To search for it, we visualize the pairing energy gap with μeV-scale energy resolution using superconductive scanning tunnelling microscopy (STM) tips26–31. We detect three PDWs, each with peak-to-peak gap modulations of around 10 μeV and at incommensurate wavevectors Pi=1,2,3 that are indistinguishable from the wavevectors Qi=1,2,3 of the prevenient24 CDW. Concurrent visualization of the UTe2 superconductive PDWs and the non-superconductive CDWs shows that every Pi:Qi pair exhibits a relative spatial phase δϕ ≈ π. From these observations, and given UTe2 as a spin-triplet superconductor12, this PDW state should be a spin-triplet PDW24,25. Although such states do exist32 in superfluid 3He, for superconductors, they are unprecedented.
Wake-like skin patterning and neural activity during octopus sleep
Authors: Pophale Aditi , Shimizu Kazumichi , Mano Tomoyuki , Iglesias Teresa L. , Martin Kerry , Hiroi Makoto , Asada Keishu , Andaluz Paulette García , Van Dinh Thi Thu , Meshulam Leenoy , Reiter Sam 
Published: June 2023

AbstractWhile sleeping, many vertebrate groups alternate between at least two sleep stages: rapid eye movement and slow wave sleep1–4, in part characterized by wake-like and synchronous brain activity, respectively. Here we delineate neural and behavioural correlates of two stages of sleep in octopuses, marine invertebrates that evolutionarily diverged from vertebrates roughly 550 million years ago (ref. 5) and have independently evolved large brains and behavioural sophistication. ‘Quiet’ sleep in octopuses is rhythmically interrupted by approximately 60-s bouts of pronounced body movements and rapid changes in skin patterning and texture6. We show that these bouts are homeostatically regulated, rapidly reversible and come with increased arousal threshold, representing a distinct ‘active’ sleep stage. Computational analysis of active sleep skin patterning reveals diverse dynamics through a set of patterns conserved across octopuses and strongly resembling those seen while awake. High-density electrophysiological recordings from the central brain reveal that the local field potential (LFP) activity during active sleep resembles that of waking. LFP activity differs across brain regions, with the strongest activity during active sleep seen in the superior frontal and vertical lobes, anatomically connected regions associated with learning and memory function7–10. During quiet sleep, these regions are relatively silent but generate LFP oscillations resembling mammalian sleep spindles11,12 in frequency and duration. The range of similarities with vertebrates indicates that aspects of two-stage sleep in octopuses may represent convergent features of complex cognition.
Fanzor is a eukaryotic programmable RNA-guided endonuclease
Authors: Saito Makoto , Xu Peiyu , Faure Guilhem , Maguire Samantha , Kannan Soumya , Altae-Tran Han , Vo Sam , Desimone AnAn , Macrae Rhiannon K. , Zhang Feng 
Published: June 2023

AbstractRNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR–Cas systems provide adaptive immunity for bacteria and archaea against foreign genetic elements. Cas effectors such as Cas9 and Cas12 perform guide-RNA-dependent DNA cleavage1. Although a few eukaryotic RNA-guided systems have been studied, including RNA interference2 and ribosomal RNA modification3, it remains unclear whether eukaryotes have RNA-guided endonucleases. Recently, a new class of prokaryotic RNA-guided systems (termed OMEGA) was reported4,5. The OMEGA effector TnpB is the putative ancestor of Cas12 and has RNA-guided endonuclease activity4,6. TnpB may also be the ancestor of the eukaryotic transposon-encoded Fanzor (Fz) proteins4,7, raising the possibility that eukaryotes are also equipped with CRISPR–Cas or OMEGA-like programmable RNA-guided endonucleases. Here we report the biochemical characterization of Fz, showing that it is an RNA-guided DNA endonuclease. We also show that Fz can be reprogrammed for human genome engineering applications. Finally, we resolve the structure of Spizellomyces punctatus Fz at 2.7 Å using cryogenic electron microscopy, showing the conservation of core regions among Fz, TnpB and Cas12, despite diverse cognate RNA structures. Our results show that Fz is a eukaryotic OMEGA system, demonstrating that RNA-guided endonucleases are present in all three domains of life.
Dynamical diagnostic of extreme events in Venice lagoon and their mitigation with the MoSE
Authors: Alberti Tommaso , Anzidei Marco , Faranda Davide , Vecchio Antonio , Favaro Marco , Papa Alvise 
Published: June 2023

AbstractExtreme events are becoming more frequent due to anthropogenic climate change, posing serious concerns on societal and economic impacts and asking for mitigating strategies, as for Venice. Here we proposed a dynamical diagnostic of Extreme Sea Level (ESL) events in the Venice lagoon by using two indicators based on combining extreme value theory and dynamical systems: the instantaneous dimension and the inverse persistence. We show that the latter allows us to localize ESL events with respect to sea level fluctuations around the astronomical tide, while the former informs us on the role of active processes across the lagoon and specifically on the constructive interference of atmospheric contributions with the astronomical tide. We further examined the capability of the MoSE (Experimental Electromechanical Module), a safeguarding system recently put into operation, in mitigating extreme flooding events in relation with the values of the two dynamical indicators. We show that the MoSE acts on the inverse persistence in reducing/controlling the amplitude of sea level fluctuation and provide a valuable support for mitigating ESL events if operating, in a full operational mode, at least several hours before the occurrence an event.
The effect of COVID-19 on nuclear medicine and radiopharmacy activities: A global survey
Authors: Al-Saeedi Fatma , Rajendran Peramaiyan , Tipre Dnyanesh , Aladwani Hassan , Alenezi Salem , Alqabandi Maryam , Alkhamis Abdullah , Redha Abdulmohsen , Mohammad Ahmed , Ahmad Fahad , Abdulnabi Yaaqoup , Alfadhly Altaf , Alrasheedi Danah 
Published: June 2023

AbstractGlobally, COVID-19 affected radiopharmaceutical laboratories. This study sought to determine the economic, service, and research impacts of COVID-19 on radiopharmacy. This online survey was conducted with the participation of employees from nuclear medicine and radiopharmaceutical companies. The socioeconomic status of the individuals was collected. The study was participated by 145 medical professionals from 25 different countries. From this work, it is evident that 2-deoxy-2-[18F]fluoro-D-glucose (2-[18F]FDG), and 99mTc-labeled macro aggregated albumin 99mTc-MAA were necessary radiopharmaceuticals used by 57% (83/145and 34% (49/145;) respondents, respectively for determining how COVID infections affect a patient’s body. The normal scheduling procedure for the radiopharmacy laboratory was reduced by more than half (65%; 94/145). In COVID-19, 70% (102/145) of respondents followed the regulations established by the local departments. Throughout the pandemic, there was a 97% (141/145) decrease in all staffing recruitment efforts. The field of nuclear medicine research, as well as the radiopharmaceutical industry, were both adversely affected by COVID-19.
iMPI: portable human-sized magnetic particle imaging scanner for real-time endovascular interventions
Authors: Vogel P. , Rückert M. A. , Greiner C. , Günther J. , Reichl T. , Kampf T. , Bley T. A. , Behr V. C. , Herz S. 
Published: June 2023

AbstractMinimally invasive endovascular interventions have become an important tool for the treatment of cardiovascular diseases such as ischemic heart disease, peripheral artery disease, and stroke. X-ray fluoroscopy and digital subtraction angiography are used to precisely guide these procedures, but they are associated with radiation exposure for patients and clinical staff. Magnetic Particle Imaging (MPI) is an emerging imaging technology using time-varying magnetic fields combined with magnetic nanoparticle tracers for fast and highly sensitive imaging. In recent years, basic experiments have shown that MPI has great potential for cardiovascular applications. However, commercially available MPI scanners were too large and expensive and had a small field of view (FOV) designed for rodents, which limited further translational research. The first human-sized MPI scanner designed specifically for brain imaging showed promising results but had limitations in gradient strength, acquisition time and portability. Here, we present a portable interventional MPI (iMPI) system dedicated for real-time endovascular interventions free of ionizing radiation. It uses a novel field generator approach with a very large FOV and an application-oriented open design enabling hybrid approaches with conventional X-ray-based angiography. The feasibility of a real-time iMPI-guided percutaneous transluminal angioplasty (PTA) is shown in a realistic dynamic human-sized leg model.
Partially substituting chemical NPK fertilizers and their impact on Eureka lemon trees (Citrus limon L. Burm) productivity and fruit quality
Authors: Almadiy Abdulrhman A. , Shaban Ayman E. , Ibrahim Ammar M. , Balhareth Salem M. , El-Gioushy Sherif F. , Khater El-Sayed G. 
Published: June 2023

AbstractThe main aim of this study is to improve productivity, physical and chemical properties of the fruits and fruit quality of Eureka Lemon trees while lowering production costs by investigating the use of different NPK alternative sources (slow release, and bio) to reduce the use of chemical NPK fertilizers. Ten treatments of NPK fertilizers were applied. The results indicate that the highest values of yield (111.0 and 114.0 kg/tree) were found with 100% chemical NPK (control) for both first and second seasons, respectively. The lemon fruit weight ranged from 131.3 to 152.4 and 131.4 to 153.5 g for first and second seasons, respectively, for all treatments under study. The highest values of fruit length and fruit diameter were found with 100% chemical NPK (control) for both two seasons. The highest values of juice quality parameters (TSS, juice acidity, TSS/acid ratio and vitamin C concentration) responded favorably to higher chemical NPK treatment rates. The highest values of TSS, juice acidity, TSS/Acid ratio, and vitamin C concentration were 9.45%, 6.25%, 1.524 and 4.27 mg/100 g, respectively, were found with 100% chemical NPK (control) for both two seasons. Meanwhile, the lowest value of total sugar was found with 100% chemical NPK (control) for both two seasons.
Maternal racism experience and cultural identity in relation to offspring telomere length
Authors: Thayer Zaneta , Becares Laia , Marks Emma , Ly Kien , Walker Caroline 
Published: June 2023

AbstractRacism is a determinant of individual and offspring health. Accelerated telomere shortening, an indicator of cellular aging, is a potential mechanism through which parental experience of racism could affect offspring. Here we longitudinally evaluated the relationship between maternal lifetime experience of an ethnically-motivated verbal or physical attack, as reported in pregnancy, with offspring telomere length in 4.5-year-old children. We also explored the potential association between positive feelings about one’s culture and offspring telomere length. Data come from a nationally representative, multi-ethnic birth cohort in Aotearoa New Zealand (NZ) (Māori N = 417, Pacific N = 364, Asian N = 381). In models adjusting for covariates, including socioeconomic status and health status, Māori mothers who experienced an ethnically-motivated physical attack had children with significantly shorter telomere length than children of Māori mothers who did not report an attack (B = − 0.20, p = 0.01). Conversely, Māori mothers who had positive feelings about their culture had offspring with significantly longer telomeres (B = 0.25, p = 0.02). Our results suggest that ethnicity-based health inequities are shaped by racism, with impacts for clinical care and policy. Future research should also evaluate the potential protective effects of positive cultural identity.
An up-conversion Ba3In(PO4)3:Er3+/Yb3+ phosphor that enables multi-mode temperature measurements and wide-gamut ‘temperature mapping’
Authors: Lei Zonghao , Liu Ronghui , Sun Lijie , Wang Xiaoxiong , Hu Chen , Zou Yanfei , Yang Xiaowei , Su Shikun , Teng Bing , Xu Hui , Zhong Degao 
Published: June 2023

Four modes of fluorescence temperature measurement were achieved by using a sample of Ba3In(PO4)3:0.02Er3+/0.05Yb3+. As the temperature increased, the emission color of the sample changed from red to green, and highest Sr value is 0.977% K−1.