bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peptide-based Self-assembly: Design, Bioactive Properties, and Its Applications
Authors: Diao He , Lu Yunhua , Ling Yun , Shen Yingjie , Yu Jingmou , Ma Kun 
Published: February 2023

Abstract:
The self-assembly of peptides is very popular in biomedical fields. Peptide-based assemblies have
been used as an ideal candidate for drug/gene delivery, tissue engineering, and antibacterial/anticancer agents.
The morphology and structure of peptide self-assembly can be changed by altering the molecular structure and
the self-assembly conditions. Engineering peptide assemblies present great potential in medical fields. In this review,
the structure and function of peptide self-assembly have been described. Also, the advances in peptide-
based self-assembly have been highlighted in biomedical applications, such as drug packaging and delivery,
tissue engineering, antibacterial agents, siRNA-targeted delivery and vaccines. Moreover, the challenges
and future perspectives of the self-assembly of polypeptides are discussed.
Tetramethylpyrazine Attenuates Oxygen-glucose Deprivation-induced Neuronal Damage through Inhibition of the HIF-1α/BNIP3 Pathway: from Network Pharmacological Finding to Experimental Validation
Authors: Xu Shixin , Zhang Nannan , Cao Lanlan , Liu Lu , Deng Hao , Hua Shengyu , Zhang Yunsha 
Published: February 2023

Aims:A network pharmacological analysis combined with experimental validation was used to investigate the neuroprotective mechanism of the natural product Tetramethylpyrazine (TMP).Background:Protecting neurons is critical for acute ischemic stroke treatment. Tetramethylpyrazine is a bioactive component extracted from Chuanxiong. The neuroprotective potential of TMP has been reported, but a systematic analysis of its mechanism has not been performed.Objective:Based on the hints of network pharmacology and bioinformatics analysis, the mechanism by which TMP alleviates oxygen-glucose deprivation-induced neuronal damage through inhibition of the HIF-1α/BNIP3 pathway was verified.Methods:In this study, we initially used network pharmacology and bioinformatics analyses to elucidate the mechanisms involved in TMP's predictive targets on a system level. The HIF-1α/BNIP3 pathway mediating the cellular response to hypoxia and apoptosis was considered worthy of focus in the bioinformatic analysis. An oxygen-glucose deprivation (OGD)-induced PC12 cell injury model was established for functional and mechanical validation. Cell viability, lactate dehydrogenase leakage, intracellular reactive oxygen species, percentage of apoptotic cells, and Caspase-3 activity were determined to assess the TMP's protective effects. Transfection with siRNA/HIF-1α or pcDNA/HIF-1α plasmids to silence or overexpress hypoxia-inducible factor 1α (HIF-1α). The role of HIF-1α in OGD-injured cells was observed first. After that, TMP's regulation of the HIF-1α/BNIP3 pathway was investigated. The pcDNA3.1/HIF-1α-positive plasmids were applied in rescue experiments.Results:The results showed that TMP dose-dependently attenuated OGD-induced cell injury. The expression levels of HIF-1α, BNIP3, and the Bax/Bcl-2 increased significantly with increasing OGD duration. Overexpression of HIF-1α decreased cell viability, increased BNIP3 expression, and Bax/Bcl-2 ratio; siRNA-HIF-1α showed the opposite effect. TMP treatment suppressed HIF-1α, BNIP3 expression, and the Bax/Bcl-2 ratio and was reversed by HIF-1α overexpression.Conclusion:Our study shows that TMP protects OGD-damaged PC12 cells by inhibiting the HIF-1α/BNIP3 pathway, which provides new insights into the mechanism of TMP and its neuroprotective potential.
Natural Cycloartane Triterpenoids from Corypha utan Lamk. with Anticancer
Activity towards P388 Cell Lines and their Predicted Interaction
with FLT3
Authors: Heliawati Leny , Kurnia Dikdik , Apriyanti Eti , Adinda Adrian Syah Putri Nabila , Lotebulo Ndruru Sun Theo Constan 
Published: February 2023

Background:
Cancer is the second leading cause of death in the world. Leukemia is a
type of cancer that accounts for 31.5% of all cancers in children under the age of 15 in industrialized
countries and 15.7% in developing countries. The inhibition of FMS-like tyrosine kinase 3 (FLT3) is
a suitable approach for acute myeloid leukemia (AML) therapy as it is overexpressed in AML.

Aim and Objective:
This study intends to explore the natural constituents from the bark of Corypha
utan Lamk., and assess their cytotoxicity on murine leukemia cell lines (P388) in addition to
predicting their interaction with FLT3 as a studied target by computational methods.

Methods:
Compounds 1 and 2 were isolated from Corypha utan Lamk using the stepwise radial
chromatography method. These compounds were assessed for their cytotoxicity against Artemia
salina using the BSLT and P388 cells and the MTT assay. The docking simulation was employed
to predict the possible interaction between triterpenoid and FLT3.

Results:
Isolation from the bark of C. utan Lamk. generated two triterpenoids, cycloartanol (1) and
cycloartanone (2). Based on the in vitro and in silico studies, both compounds were found to have anticancer
activity. The evaluation of cytotoxicity from this study reveals that cycloartanol (1) and cycloartanone
(2) could inhibit P388 cell growth (IC50 value at 102.6 and 110.0 μg/mL, respectively). The binding
energy of cycloartanone was -9.94 Kcal/mol with a Ki value of 0.051 μM, while the binding energy
and Ki value of cycloartanol (1) were found to be 8.76 Kcal/mol and 0.38 μM, respectively. These compounds
also demonstrate a stable interaction by forming hydrogen bonds with FLT3.

Conclusion:
Cycloartanol (1) and cycloartanone (2) exhibit potency as anticancer agents by inhibiting
P388 cells in vitro and the FLT3 gene in silico.
Effect of the Application of Exosome on Gastric Cancer
Authors: Peng Jing , Wu Yiwen , Deng Sijun , Cao Pu , Yang Wei , Gong Tingyi , Lei Xiaoyong , Yang Xiaoyan 
Published: February 2023

Abstract:
Gastric cancer is one of the most common and highest mortality rate cancers in the
world. Exosomes are vesicles secreted by cells carrying different types of molecules, such as protein
and RNA. Numerous studies have confirmed that exosomes are involved in various stages of
the occurrence and development of gastric cancer and play an important role. With the gradual development,
exosomes have been widely employed in the diagnosis and treatment of gastric cancer.
In this review, we have provided a basic overview of exosome, and discussed the role of exosome
in the occurrence, proliferation, invasion, metastasis, and drug resistance in gastric cancer. In addition,
we have emphasized the bright development prospect of exosome in the diagnosis and treatment
of gastric cancer. The data on the discovery, diagnosis, treatment, and prognosis of gastric
cancer are not particularly optimistic, but the discovery of exosome, applied in diagnosis and
treatment, provides a new and effective way to improve the survival rate of patients with gastric
cancer.
Current Perspectives on Paclitaxel: Focus on Its Production, Delivery and
Combination Therapy
Authors: Liu Yibin , Zhao Fenglan , Wang Qibao , Zhao Qingjie , Hou Guige , Meng Qingguo 
Published: February 2023

Abstract:
Paclitaxel is an anticancer drug first isolated from the bark of the Pacific yew tree. It has
been widely used for the treatment of ovarian, breast, uterine and other cancers because of its low toxicity,
high efficiency and broad-spectrum anticancer activity, and it is considered to be one of the most
successful natural anticancer drugs available. Paclitaxel is a microtubule-targeting drug whose main
molecular mechanism is to disrupt microtubule dynamics and induce mitotic arrest and cell death. Despite
the many clinical successes of paclitaxel, the extraction of natural paclitaxel from Taxus species
has proven to be environmentally unsustainable and economically unviable. As a result, researchers
are constantly working to find innovative ways to meet society's need for this drug. Currently, many
methods, including artificial cultivation, microbial fermentation, chemical synthesis, and tissue and
cell culture, have been explored and developed to obtain paclitaxel. In addition, the poor water solubility
of paclitaxel has led to significant limitations in its clinical application. Conventional paclitaxel
formulations use Cremophor EL and ethanol to dissolve paclitaxel, which can lead to serious side effects.
In recent decades, a series of new nanotechnology-based paclitaxel dosage forms have been developed,
including albumin-bound paclitaxel, polymeric micellar paclitaxel, polymer-paclitaxel couples,
and liposome-encapsulated paclitaxel. These nanoformulations can significantly reduce the toxicity
of paclitaxel and greatly improve its anti-tumor efficiency. This paper reviews the development
of the production, dosage form and combination therapy of paclitaxel in recent years and presents an
outlook, with the aim of providing a theoretical basis and reference for further research on the production
and application of paclitaxel in the future.
Role of Human Papillomavirus in Various Cancers: Epidemiology, Screening
and Prevention
Authors: Ahmed Waqas , Zaib Sumera , Ullah Sami , Fatima Areej , Zaib Zainab , Haseeb Azam Muhammad Abdul , Umar Usama , Tariq Muhammad Umair , Aslam Rabia , Khan Imtiaz 
Published: February 2023

Abstract:
It is estimated that viral infections contribute 15-20% of all human cancers. Several types
of human papillomaviruse (HPV) are related to the development of many cancer types and their
treatment. About 200 HPV viruses have been identified, and each type of virus is integrated with a
certain type of clinical lesions affecting the areas of skin and mucous membranes. Infections associated
with HPV are known to cause multiple cancer types such as uterine cancer, cervical, vaginal carcinoma,
and other varieties of carcinomas such as genital and oropharyngeal, penile, and short-lived
carcinomas. Most of the HPV types are considered as high-risk mutants that can provoke cervical
cancer in females in addition to significant contribution to other cancer types such as anogenital cancer
and tumor in head and neck regions. Among them, high risk human papilloma viruses (HR-HPV)
subtypes 16 and 18 play a major role in the etiology of cervical cancer worldwide. Although, cervical
cancer incidence and fatality rates vary greatly depending on geographical area, it is the leading cause
of mortality in women around the world. In addition, it is epidemiologically similar to a sexually
transmitted disease of low infectivity. In this review article, the association of HPV with different
types of cancers have been explained, but the main focus remains on cervical cancer.
Non-consumption of Meat and Health Status in Italy: 2013-2016 Snapshot
Authors: Barbadoro Pamela , Ponzio Elisa , D'Errico Matteo , Dolcini Jacopo , M. D’Errico Marcello 
Published: February 2023

Background:
A large number of individuals around the world consider themselves to be vegetarians for many individual reasons, and the vegetarian diet appears very heterogeneous. The prevalence of vegetarianism varies widely around the world.

Objective:
This paper examined the variables associated with the non-consumption of meat and valued trends in the plant-based diet among Italian adults in relation to the level of self-perceived health in this category of people in 4 years (2013-2016).

Method:
Data were drawn from the survey “Aspects of daily life” conducted by the ISTAT (Istituto Nazionale di Statistica). We considered the data of the 2013-2016 editions to evaluate the trend and attitude of the Italian population towards the non-consumption of meat during 4 years and assessed trends in the plant-based diet among Italian adults in relation to the level of self-perceived health.

Results:
The results showed an increasing trend from 2013 to 2016. In statistical analysis, vegetarian status was associated to under the age of 45, having a high level of education, being mainly separated or divorced, being resident in the North-West regions, and with a poor self-perceived state of health. Furthermore, vegetarians had shown healthier lifestyles and lower risk factors for non-communicable diseases than non-vegetarians.

Conclusion:
The results suggest that despite perceiving a worse health status, the long-term health of vegetarians is good and may be better than that of comparable non-vegetarians for some conditions and diseases, such as Obesity and IHD. As for all types of diet, well-designed health educational interventions conveying information on correct eating behavior may significantly contribute to increasing the degree of perceived health.

conclusion:
The results suggest that despite perceiving a worse health status, the long-term health of vegetarians is good, and may be better than that of comparable non-vegetarians for some conditions and diseases such as obesity and IHD.

other:
As for all types of diet, well-designed health educational interventions conveying information on correct eating behavior may significantly contribute to increasing the degree of perceived health.
CT and MRI Findings of Solitary Extramedullary Plasmacytoma: A
Series of Ten Case Reports and Review of Literature
Authors: Ding Hao , Mo Zhi-qing , Xu Hua , Zhang You-ming , Yuan Wen-zhao , Zeng Zi-san 
Published: February 2023

Objective:
The study aimed to analyze computed tomography and magnetic resonance imaging
features of patients with solitary extramedullary plasmacytoma.

Results:
This study included both males (n = 8) and females (n = 2), with a median age of 48 (range
21-72 years old). The organs or anatomical structures involved were nasopharynx (n = 3), orbit (n = 1),
left tentorium (n = 1), nasal cavity and meatus (n = 2), small intestine and mesentery (n = 1), as well as
posterior thoracic wall (n = 2). The median maximum diameter of the tumors was 3.2 cm (range 0.8-
15.2 cm). The tumor shapes were round (n = 7), stripped (n = 1), irregular (n = 1), and nodular (n = 1).
The margin of the tumors was well-defined (n = 7) and partially well-defined (n = 3). There were 3
cases with bone destruction and 1 with a tissue invasion adjacent to the tumor, calcification (n = 1),
and cystic degeneration (n = 2). Enlarged tumoral vessels (n = 4) could also be observed. The CT attenuation
and MR signal intensity of tumors were heterogeneous (n = 4) and homogenous (n = 6). After
the injection of the contrast agent, marked (n = 5), mild (n = 1), mild to moderate (n = 1), and delayed
enhancement (n = 1) could be observed.

Conclusion:
A well-defined homogeneous solitary mass occurring at the head and neck with a marked
enhancement, an adjacent tissue invasion, enlarged tumoral vessels, and bone destruction can indicate
the diagnosis of solitary extramedullary plasmacytoma.
Longitudinal Association between L1 Trabecular Attenuation from Chest
Computed Tomography (CT) and Bone Mineral Density from Dualenergy
X-ray Absorptiometry (DXA)
Authors: Lim Jiyun , Oh Eunsun , Park Suyeon , Kim Hyun-Joo , Yoon Young Cheol , Nam Boda , Lee Eun Ji , Hwang Jiyoung , Jeong Jewon , Chang Yun-Woo 
Published: February 2023

Background:
Many studies have shown that vertebral trabecular attenuation measured on
CT scan corresponds well to DXA results for bone mineral density. These studies were based on crosssectional
data. Hence, there were limitations in explaining the constantly changing vertebral trabecular
attenuation from CT and T-score from DXA over time.

Objective:
This study aimed to determine the longitudinal association between the vertebral trabecular
attenuation measured on computed tomography (CT) and the T-score measured by dual-energy X-ray
absorptiometry (DXA).

Methods:
We performed a database search for 333 patients who underwent surgery for breast cancer,
preoperative treatment, and at least one follow-up chest CT and DXA from January, 2013 through
May, 2021. One musculoskeletal radiologist measured the mean vertebral trabecular attenuation of
lumbar vertebra 1(L1) on axial unenhanced images at the pedicle level by manually placing the region
of interest (ROI). DXA of the lumbar spine was performed, and the lowest T-score of the lumbar spine
was used for the analysis. We evaluated the association between L1 trabecular attenuation from chest
CT and T-score from DXA over time using the generalized estimating equations (GEE) model to analyze
longitudinal corrected data.

Results:
A total of 150 women (mean age, 52.4 ± 11.0 years) were included. There was a statistically
significant association between L1 trabecular attenuation from chest CT and T-score from DXA in the
unadjusted model (p < 0.001) and adjusted model (p < 0.001). T-score value increased by 0.172 (95%
confidence interval (CI): 0.145-0.200, p < 0.001) per 10 unit (HU) of L1 trabecular attenuation at time
= 0 in unadjusted model and by 0.173 (95% CI: 0.143-0.203, p < 0.001) in all adjusted model.

Conclusion:
We demonstrated that L1 attenuation from chest CT images was longitudinally associated
with T-score from DXA, and the degree of association appeared to be decreased over time in breast
cancer patients regardless of their medical condition.
Review of Research on Improved PID Control in Electro-hydraulic Servo
System
Authors: LI Jianying , Li Weidong , Liang Heng , Kong Lingbing 
Published: February 2023

Background:
Proportional-integral-differential (PID) controller is widely used in the engineering
field because of its simple structure, high control accuracy, and easy operation. Different patented
PID control technologies have their own advantages, disadvantages, and application scenarios.

Objective:
By analyzing and discussing the improved PID control techniques in the electrohydraulic
servo system, some valuable conclusions have been drawn to predict the future research
and development of PID control techniques.

Methods:
The improved PID control techniques applied to electro-hydraulic servo systems are classified
into three categories: PID parameter tuning technology, PID parameter online adjustment
strategy and compound control strategy combined with PID algorithm.

Results:
By comparing the principles and characteristics of the above techniques, the advantages,
disadvantages and application scenarios of each are analyzed, and the further development of PID
control technology is discussed.

Conclusion:
Based on the characteristics of electro-hydraulic servo systems, the further combination
of the three types of technology can make up for the shortcomings of PID algorithms to form a
control strategy with high control accuracy, high robustness, and fast response speed.
Fangchinoline, an Extract of the Stephania tetrandra S. Moore Root, Promoted
Oxidative Stress-induced DNA Damage and Apoptosis and Inhibited Akt
Signaling in Jurkat T Cells
Authors: Xu Yubo , Shao Yanxiong , Li Chaoran , Miao Guojun 
Published: February 2023

Background:
Fangchinoline (Fan) is extracted from traditional Chinese medicine (called Fangji), or the root of Stephania tetrandra Moore. Fangji is well-known
in Chinese medical literature for treating rheumatic diseases. Sjogren's syndrome (SS) is a rheumatic disease whose progression can be mediated
via CD4+ T cell infiltration.

Objective:
This study identifies the potential role of Fan in inducing apoptosis in Jurkat T cells.

Methods:
First, we explored the biological process (BP) associated with SS development by performing a gene ontology analysis of SS salivary gland-related
mRNA microarray data. The effect of Fan on Jurkat cells was investigated by analyzing the viability, proliferation, apoptosis, reactive oxygen
species (ROS) production, and DNA damage.

Results:
Biological process analysis showed that T cells played a role in salivary gland lesions in patients with SS, indicating the significance of T cell
inhibition in SS treatment. Viability assays revealed that the half-maximal inhibitory concentration of Fan was 2.49 μM in Jurkat T cells, while the
proliferation assay revealed that Fan had an inhibitory effect on the proliferation of Jurkat T cells. The results of the apoptotic, ROS, agarose gel
electrophoresis, and immunofluorescence assays showed that Fan induced oxidative stress-induced apoptosis and DNA damage in a dosedependent
manner.

Conclusion:
These results indicate that Fan could significantly induce oxidative stress-induced apoptosis and DNA damage and inhibit the proliferation of
Jurkat T cells. Moreover, Fan further enhanced the inhibitory effect on DNA damage and apoptosis by inhibiting the pro-survival Akt signal.
Challenges and Opportunities of the Use and Adoption of Telemedicine for Diabetes Care and Management During the COVID-19 Pandemic and Beyond
Authors: Altabtabaei Rabab , Alhuwail Dari 
Published: February 2023

Background
More than half of the world’s population deals with noncommunicable diseases causing premature death. Leveraging digital solutions like telemedicine, health care providers (HCPs) can provide medical care remotely. Yet, there is little known about the contextual challenges and opportunities of leveraging telemedicine solutions in varying socioeconomic and cultural contexts, including Kuwait.

Objective
The aims of this paper were as follows: (1) uncover the challenges and opportunities of adopting and using telemedicine for diabetes care and management from the viewpoints of HCPs and patients with diabetes; (2) explore nonfunctional requirements for telemedicine applications for diabetes care and management; and (3) offer recommendations to improve the adoption of telemedicine in Kuwait’s health care system for diabetes care and management.

Methods
Through semistructured interviews, this study employed a qualitative and exploratory design to uncover rich context-specific findings. Participants were recruited via social media platforms. The analysis followed a thematic analysis approach and used the framework method. Researchers used the “diffusion of innovation” model as a lens to guide the analysis and interpretation of the results.

Results
A total of 20 interviews were conducted—10 (50%) HCPs and 10 (50%) patients with diabetes. The participants were familiar with and interested in adopting telemedicine. Challenges included a lack of telemedicine infrastructure and how to increase patients’ technology awareness. Patients with diabetes mentioned that telemedicine would save time and effort. The participants suggested developing a secure, user-friendly telemedicine solution. They stated the importance of telemedicine during the pandemic, as many diabetes cases can be followed up online, which reduces virus spread and increases patients’ safety.

Conclusions
The findings from this study can give a better understanding of what HCPs and patients with diabetes need to accept the adoption of telemedicine in resource-rich countries like Kuwait. The COVID-19 pandemic impacted the ways HCPs deliver medical care to patients and encouraged both HCPs and patients to explore the digital platform for continuous care and management of diabetes.

Conflicts of Interest
None declared.