bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wolfram syndrome 1b mutation suppresses Mauthner-cell axon regeneration via ER stress signal pathway
Authors: Wang Zongyi , Wang Xinliang , Shi Lingyu , Cai Yuan , Hu Bing 
Published: December 2022

AbstractWolfram Syndrome (WS) is a fatal human inherited disease with symptoms of diabetes, vision decreasing, and neurodegeneration caused by mutations in the endoplasmic reticulum (ER)-resident protein WFS1. WFS1 has been reported to play an important role in glucose metabolism. However, the role of WFS1 in axonal regeneration in the central nervous system has so far remained elusive. Herein, we established a model of the wfs1b globally deficient zebrafish line. wfs1b deficiency severely impeded the Mauthner-cell (M-cell) axon regeneration, which was partly dependent on the ER stress response. The administration of ER stress inhibitor 4-Phenylbutyric acid (4-PBA) promoted M-cell axon regeneration in wfs1b−/− zebrafish larvae, while the ER stress activator Tunicamycin (TM) inhibited M-cell axon regeneration in wfs1b+/+ zebrafish larvae. Moreover, complementation of wfs1b at the single-cell level stimulated M-cell axon regeneration in the wfs1b−/− zebrafish larvae. Altogether, our results revealed that wfs1b promotes M-cell axon regeneration through the ER stress signal pathway and provide new evidence for a therapeutic target for WS and axon degeneration.
Brain autopsies of critically ill COVID-19 patients demonstrate heterogeneous profile of acute vascular injury, inflammation and age-linked chronic brain diseases
Authors: Agrawal Sonal , Farfel Jose M. , Arfanakis Konstantinos , Al-Harthi Lena , Shull Tanner , Teppen Tara L. , Evia Arnold M. , Patel Mayur B. , Ely E. Wesley , Leurgans Sue. E. , Bennett David A. , Mehta Rupal , Schneider Julie A. 
Published: December 2022

Abstract
Background
This study examined neuropathological findings of patients who died following hospitalization in an intensive care unit with SARS-CoV-2.

Methods
Data originate from 20 decedents who underwent brain autopsy followed by ex-vivo imaging and dissection. Systematic neuropathologic examinations were performed to assess histopathologic changes including cerebrovascular disease and tissue injury, neurodegenerative diseases, and inflammatory response. Cerebrospinal fluid (CSF) and fixed tissues were evaluated for the presence of viral RNA and protein.

Results
The mean age-at-death was 66.2 years (range: 26–97 years) and 14 were male. The patient’s medical history included cardiovascular risk factors or diseases (n = 11, 55%) and dementia (n = 5, 25%). Brain examination revealed a range of acute and chronic pathologies. Acute vascular pathologic changes were common in 16 (80%) subjects and included infarctions (n = 11, 55%) followed by acute hypoxic/ischemic injury (n = 9, 45%) and hemorrhages (n = 7, 35%). These acute pathologic changes were identified in both younger and older groups and those with and without vascular risk factors or diseases. Moderate-to-severe microglial activation were noted in 16 (80%) brains, while moderate-to-severe T lymphocyte accumulation was present in 5 (25%) brains. Encephalitis-like changes included lymphocytic cuffing (n = 6, 30%) and neuronophagia or microglial nodule (most prominent in the brainstem, n = 6, 30%) were also observed. A single brain showed vasculitis-like changes and one other exhibited foci of necrosis with ball-ring hemorrhages reminiscent of acute hemorrhagic leukoencephalopathy changes. Chronic pathologies were identified in only older decedents: 7 brains exhibited neurodegenerative diseases and 8 brains showed vascular disease pathologies. CSF and brain samples did not show evidence of viral RNA or protein.

Conclusions
Acute tissue injuries and microglial activation were the most common abnormalities in COVID-19 brains. Focal evidence of encephalitis-like changes was noted despite the lack of detectable virus. The majority of older subjects showed age-related brain pathologies even in the absence of known neurologic disease. Findings of this study suggest that acute brain injury superimposed on common pre-existing brain disease may put older subjects at higher risk of post-COVID neurologic sequelae.
Beliefs and self-reported practice of footcare among persons with type II diabetes mellitus attending selected health centres in east Trinidad
Authors: Vincent-Edinboro Rachel Leah , Onuoha Philip 
Published: December 2022

Abstract
Background
It has been documented that nearly 600 million people worldwide are expected to have diabetes mellitus in 2035 and that approximately 140,000 persons aged 20–69 years living with diabetes mellitus in Trinidad and Tobago. It is also indicated that patients with type II diabetes mellitus face a higher risk of diabetic foot neuropathy and foot ulcers which increase the risk of below the knee amputation in persons living with diabetes.

Purpose
The aim of this research project was to explore the beliefs related to footcare and the self-reported footcare practice of persons with type II diabetes mellitus attending selected health centres in East Trinidad.

Method
A survey was used to explore the footcare beliefs and the self-reported footcare practice of persons with type II diabetes mellitus attending selected health centres in East Trinidad (n = 87).

Results
Participants had strong belief regarding susceptibility to foot injury, strong belief regarding the seriousness of complications of foot injury, reported good footcare practice and excellent practice of overcoming barriers to performing footcare. There is a correlation between the belief regarding susceptibility to a foot injury and age (p ≤ 0.05). Also, there is a significant correlation between belief regarding susceptibility to a foot injury, seriousness of complications and self-reported footcare practices (p ≤ 0.05).

Conclusion
This study explores and describes the beliefs and self-reported practices of footcare among individuals with type II diabetes mellitus at selected health centres in East Trinidad. It supports the Health Belief Model as an effective framework for the promotion of appropriate footcare among persons with type II diabetes mellitus.
Treatment of full-layer skin defects using decellularized pig skin dermal matrix
Authors: Kulianda О. , Kulianda О. , Denefil О. 
Published: December 2022

The choice of treatment methods for complex skin defects poses certain technical and medical difficulties related to the choice of material for filling the wound defect and its antigenic compatibility. A promising direction in the treatment of skin defects is the use of decellularized (celluless) pig skin, the composition and structure of which is as close as possible to the patient's dermis.
The aim of the study was to analyze the clinical case of a patient with a basal cell carcinoma in the right wing of the nose, who was treated with full-layer skin defects using a decellularized dermal matrix from pig skin.
Material and methods of investigation. Patient P., 94 years old, with a basal cell carcinoma in the area of the right wing of the nose, underwent treatment. As a material applied directly to the wound, a flap of acellular dermal matrix (ADM) of pig skin with preservation of the native structure was used.
Results. 6 weeks before the visit to the dermatologist, the patient developed a pea-sized papule on the skin in the area of ​​the wing of the nose, which increased in size and became painful on palpation. When examining the skin in the area of ​​the right wing of the nose, a tumor-like formation was observed, 1x1 cm in size, flesh-colored, with peeling on the surface and vascularization, firm consistency, painful. Regional lymph nodes are not enlarged. The formation is clearly separated from healthy skin. The oncologist made a diagnosis: basal cell carcinoma. The tumor was removed, resulting in a full-layer skin defect measuring 1x1.5 cm with connection to the nasal cavity. Acellular dermal matrix was used to close the defect on the 3rd day after surgery. The flap was placed so that it did not cover the skin around the wound. With the help of "Levomykol" ointment, a moist chamber was created over the dermal flap. The dressing was changed every 2-3 days. On the 14th day, the flap peeled off, a granulating wound with signs of marked marginal epithelization formed in its place. The bottom of the wound was located almost at the same level as the intact skin. On the 21st and 26th day, active marginal epithelization of the wound and wound healing under the scab were noted. The bandage was not used during this period. On the 32nd day, the wound closed.
Conclusions. Decellularized dermal matrix of pig skin when closing a full-layer postoperative skin defect creates optimal conditions for reparative wound regeneration. The effectiveness of its use is due to the presence of a collagen matrix in it, from which the nuclear elements of cells have been removed, which does not lead to immune aggression. The clinical case proves the expediency of using the decellularized dermal matrix of pig skin in the treatment of full-layer skin defects of various genesis.
Direktni psihosocialni dejavniki tveganja za zdravje zaposlenih v bolnišnici
Authors: Rus Tanja , Peršolja Melita 
Published: December 2022

Uvod: V delovnem okolju na zdravje zaposlenih vplivajo indirektni in direktni psihosocialni dejavniki tveganja, ki določajo storilnost in učinkovitost zaposlenega. Namen raziskave je bil ugotoviti moč povezanosti direktnih dejavnikov tveganja pri delu na psihofizično zdravje zaposlenih v bolnišnici.Metode: Izvedena je bila presečna raziskava na 112 izmed 994 naključno izbranih zaposlenih v splošni bolnišnici. Kot merski instrument je bil uporabljen vprašalnik Obvladovanje psihosocialnih tveganj in absentizma (Cronbach α = 0,915). S kvantitativno analizo podatkov je bila raziskana povezanost med spremenljivkami in z regresijsko analizo moč neodvisnih spremenljivk na psihofizično zdravje zaposlenih.Rezultati: Anketiranci so izmed težav z zdravjem najpogosteje navedli bolečine v mišicah ramen, vratu in/ ali zgornjih udih (n = 44, 39,3 %), počutijo se preobremenjeni in utrujeni (n = 52, 46,6 %). S psihofizičnimzdravjem zaposlenih se značilno povezujejo štirje od šestih direktnih psihosocialnih dejavnikov tveganja, in sicer so ti delovno okolje, delovna oprema in fizične obremenitve (rs = 0,385, p < 0,01); obremenitve kot posledica sociodemografskih okoliščin (rs = 0,401, p < 0,01); delovna obremenitev in hitrost poteka dela (rs = 0,319, p < 0,01) in; odnos zaposlenega z nadrejenimi (rs = 0,261, p < 0,01). Psihofizično zdravje pojasnjujejo (R2 = 0,18, p < 0,01) delovno okolje, delovna oprema in fizične obremenitve (R2 = 0,15, p < 0,05) ter obremenitve kot posledica sociodemografskih okoliščin (R2 = 0,08, p < 0,05).Diskusija in zaključek: Anektirani potrebujejo več neposrednega nadzora, boljše upoštevanje njihovega mnenja pri odločanju na nivoju organizacije in večjo prilagoditev časovnih rokov pri delu. Preobremenjenost in utrujenost izhajata predvsem iz nočnega dela, najpogostejša težava z zdravjem – bolečine v vratu, ramenih in rokah – pa je povezana z dvigovanjem bremen in prekratkimi odmori med delom.
Zadovoljstvo pacientov z dostopnostjo zdravstvenih storitev v obdobju epidemije covid-19
Authors: Plahuta Ana , Skrt Sanja , Jazbec Julija , Prosen Mirko , Ličen Sabina 
Published: December 2022

Uvod: Hitro širjenje nove vrste koronavirusa (SARS-CoV-2) in visoko število hospitalizacij sta ustavila javno življenje na globalni ravni. Zaradi omejevanja gibanja v času pandemije je prišlo do omejitev obiskovanja zdravstvenih ustanov in izvajanja neposrednega zdravstvenega varstva. Namen raziskave je bil ugotoviti zadovoljstvo pacientov z dostopnostjo do zdravstvenih storitev v času epidemije covida-19 v Sloveniji.Metode: Uporabljena je bila opisna neeksperimentalna metoda empiričnega raziskovanja. Merski instrument je predstavljal spletni vprašalnik, ki ga je med decembrom 2020 in februarjem 2021 izpolnilo 226 oseb. Uporabljen je bil priložnostni vzorec odraslih oseb. Podatki so bili analizirani z opisno statistiko ter neparametričnim Mann-Whitneyjevim U-testom in Kruskal-Wallisovim testom.Rezultati: Anketiranci najbolj cenijo prijazen in spoštljiv odnos zdravstvenih delavcev ter razpoložljivost za hitro pomoč. Negativno ocenjujejo telefonsko nedosegljivost, čakanje v čakalnicah ter kratek čas obravnave pri zdravniku. Rezultati nakazujejo manjšo stopnjo zadovoljstva z dostopnostjo zdravstvenih storitev v časuepidemije covida-19 (Me = 86,50). Rezultati kažejo pozitivno stališče moških o dostopnosti zdravstvenih storitev (U = 1297,5, p < 0,05), med ostalimi skupinami ni bilo statistično pomembnih razlik (p < 0,05). Diskusija in zaključek: Ugotovite kažejo, da se v času od pojava virusa SARS-CoV-2 v Sloveniji zaupanje do zdravstva in zdravstvenih storitev in njuna dostopnost nista izrazito spremenila. Ponekod po svetu se je v tem času še dodatno razvilo področje telemedicine, ki jo je treba tudi v Sloveniji bolj uveljaviti. V prihodnje je smiselno raziskati, kako je epidemija vplivala na psihofizično zdravje ljudi v naši državi.
Izkušnje kronično obolelih pacientov s telemedicinsko obravnavo v ambulantah družinske medicine
Authors: Prevodnik Katja , Hvalič-Touzery Simona , Dolničar Vesna , Zaletel Jelka , Laznik Jerneja , Petrovčič Andraž 
Published: December 2022

Uvod: Telemedicinska obravnava stopa v ospredje kot strategija za obvladovanje kroničnih nenalezljivih bolezni, ki v Sloveniji povzročijo visok delež smrti. Namen raziskave je bil preučiti, kakšne izkušnje imajo pacienti z diagnozo kronične bolezni tako z uporabo telemedicinske opreme kot s telemedicinsko obravnavo v ambulanti družinske medicine.Metode: Julija 2020 so bile v enem od slovenskih zdravstvenih domov izvedene štiri fokusne skupine z 19 pacienti s sladkorno boleznijo tipa 2 in/ali visokim krvnim tlakom. Prepisi skupinskih pogovorov so bili analizirani po načelih induktivne tematske analize.Rezultati: S pomočjo induktivne tematske analize je bilo oblikovanih sedem tem: prednosti in slabosti telemedicinske obravnave, enostavnost uporabe telemedicinske opreme, dvosmerna komunikacija z zdravstvenim osebjem, učinki telemedicinske obravnave na zdravstveno stanje pacientov, želja po uporabi in dostopnosti telemedicinske obravnave, vpliv in opora okolja ter skrb za lastno zdravje. Teme vsebujejo 10 podtem in 29 dejavnikov.Diskusija in zaključek: Na izvajanje telemedicinskih meritev najbolj vplivajo zaznana potreba pacientov in navodila zdravstvenega osebja. Kljub pozitivni izkušnji pacientov z uporabo telemedicinske opreme je potrebna strokovna presoja primernosti tovrstne obravnave na individualni ravni, ki ne vključuje le zdravstvenega stanja pacientov, marveč tudi oceno njihovega odnosa do zdravja in digitalnih veščin.
Konflik Sosial Tokoh Utama dalam Film Animasi Haikyuu!! Second Season (Kajian Sosiologi Sastra)
Authors: Nurfajriyati Niken Sekar Ayu , Seruni Ayu Putri 
Published: December 2022

Social conflict was defined as a natural social symptom that occurs in social life. Coser divided conflicts into several parts, there are realistic, non- realistic, in- group and out- group conflicts. He argues that social conflict not only viewed from the negative side. This research focuses on social conflicts that occur the Japanese student environment then will analyze more deeply the social conflicts experienced by the main character also will look for the factors that occurs the conflicts. Thie aim of this studies to explains and describing (1) realistic and non- realistic conflicts that occur (2) in-group and out-group conflicts that occur (3) functions of social conflicts. This study used content analysis of qualitative research methods with literary sociology approach. For data collecting technique this study used note- taking technique. The result of this research is (1) the occurrence of realistic conflicts due to a sense of disapproval of a demand (2) the occurrence of non- realistic conflicts due to the need to relieve tensions (3) the occurrence of in-group conflicts because those involved in the conflicts are between member in the same groups (4) the occurrence of out-group conflicts because those involved in the conflicts are between different groups.
Příroda, biologie a nové technologie v umění
Authors: Kubíčková Jindra 
Published: December 2022

Proud umění, v němž se setkává biologie s uměleckou tvorbou, se výrazněji objevuje především na konci dvacátého století, kdy biologie – zejména v podobě genetiky, biochemie či biotechnologií – začala otevírat nedozírné možnosti nejen pro vědce, ale i současné umělce. Biologické inspirace v umění jsou dnes označovány několika pojmy, mezi které patří bio art, transgenní umění či biologické umění a další. Přitom není vždy snadné rozeznat hranice nejen mezi jednotlivými označeními, ale i samotnými uměleckými díly. Často je ostatně obtížné určit v této tendenci hranice mezi vědou a uměním, což je také charakteristický rys práce umělců, kteří se inspirují biotechnologickými postupy. Termín bio art (někdy též Bio-Art nebo Bio Art) se zpravidla používá pro umělecká díla, jejichž hlavním tématem jsou živé organismy či pro díla vytvořená ve spolupráci s těmito organismy.
Multiplicity and minimality of periodic solutions to fourth-order super-quadratic difference systems
Authors: Ling Rumin , Lu Mingxi , Feng Yuncheng , Xiao Huafeng 
Published: December 2022

Abstract
In this article, we use the Nehari manifold method to study a class of fourth-order even difference systems. First, we show that there exist multiple periodic solutions to the non-autonomous system. Second, we obtain sufficient conditions to guarantee the existence of solutions with prescribed minimal periods to the autonomous system. Our results generalize the results in reference.
Validation Technique for Heavy Vehicle Simulator Rut Measurement with Florida Laser Rut Meter
Authors: Khan Shahbaz , Cotter Chris , Greene James 
Published: December 2022

Abstract
The performance of a pavement system is typically measured in structural and functional terms. Agencies often prioritize minimizing rutting as it indicates the mixture and structural inadequacy and can lead to safety concerns such as hydroplaning. Therefore, accurate rut depth measurement is critical to pavement management as well as to assess the performance of materials designed to resist rutting. To that measure, Florida Department of Transportation (FDOT) uses two heavy vehicle simulators (HVSs) as part of an accelerated pavement testing (APT) program to advance pavement research and implement new pavement materials, designs, and construction methods. Each HVS device is equipped with an on-board laser profiling system for rut depth measurement. To ensure accurate and reproducible rut depth measurements, a laser-based reference device was developed and constructed by FDOT to verify rut depth measurements made by both HVS devices during APT research studies. This article describes the rut depth reference device, the verification process, and data analysis methods used to validate the accuracy of the HVS devices.
Impact of Synbiotic Therapy on the Quality of Life in Patients with Mild-to-Moderately Active Ulcerative Colitis
Authors: Kamarli Altun Hulya , Akal Yildiz Emine , Akin Mete 
Published: December 2022

Background and Aims: Ulcerative colitis is a chronic inflammatory disease which is affecting the quality of life, workforce and nutrition of patients negatively in the active periods due to its symptoms. This study aims to explore how synbiotic treatment affects the quality of life in patients with mild-to-moderate ulcerative colitis.
Methods: Forty ulcerative colitis patients with mild-to-moderate activity were enrolled in the study. Patients were split into two even randomized groups as synbiotic (20 patients) and control (20 patients). The synbiotic group received synbiotic therapy and the control group received placebo for 8 weeks. Both groups were compared at the start and the end of therapy according to the quality of life scores. Quality of life was determined using a short form-36 (SF-36) questionnaire.
Results: An increase in mean SF-36 scores were found in both groups at the end of the study. Altough this increase was higher in patients received synbiotic therapy, the difference was not statistically significant (p > 0.05). SF-36 scores were higher in patients with mild activity or those in remission in both groups.
Conclusion: Synbiotic use provides an increase in the SF-36 score, however, this increase is not statistically significant.
Somatostatinoma of the Ampulla of Vater: A Systematic Review
Authors: Giannakodimos Ilias , Giannakodimos Alexios , Ziogou Afroditi , Frountzas Maximos , Kritikos Neoklis , Vlachos Konstantinos , Toutouzas Konstantinos G , Schizas Dimitrios 
Published: December 2022

Background and Aims: Somatostatinoma of the ampulla of Vater (SAV) is a rare neuroendocrine tumor that usually appears with atypical clinical manifestations and is associated with Von Recklinghausen’s disease. The aims of this study were to systematically review the literature regarding SAV and to highlight the clinicopathological characteristics and optimal therapeutic management of this rare entity.
Methods: A systematic search of the literature in PubMed/Medline and Scopus databases was performed by two independent investigators, including all case reports and case series concerning SAVs from 1980 until September 2021.
Results: In total, 37 articles were retrieved, including 43 patients, with a male to female ratio of 1.8:1 and a mean age of 46.8 ± 11.3 years (mean, SD). For 23 out of 43 patients (53.5%), Von Recklinghausen’s disease was proved. The main clinical manifestations were abdominal pain (41.9%), jaundice (27.9%), weight loss (20.9%) and bowel disorders (20.9%). Typical histological findings included psammoma bodies, nests or clusters of epithelial cells with eosinophilic cytoplasm, while somatostatin staining was positive in 35 patients (81.4%), chromogranin-A in 21 patients (48.8%) and synaptophysin in 18 patients (41.9%). Surgery was the initial therapeutic approach in 34 patients (79.1%), whereas Whipple’s procedure was the preferred surgical approach in 23 patients (53.4%). The longest survival among included patients was 13 years and only two postoperative deaths (4.7%) were reported.
Conclusions: Somatostatinomas of the ampulla of Vater are rare malignancies that require increased physicians’ suspicion and accurate surgical approach in order to achieve optimal therapeutic results.