bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility of Recruiting and Retaining Adolescents into a Prospective Cohort Study of the Effects of Social Isolation During COVID-19
Authors: Kim Kain , Jergel Andrew , Bai Shasha , Bradley Kolbi , Karim Brianna , Shah Amit , Suglia Shakira , Ugboh Ngozi , Gooding Holly C. 
Published: May 2023

Abstract
Background Social connection throughout the life course is increasingly understood as critically important for mental and physical health. We sought to investigate the feasibility of using ecological momentary assessment (EMA) and wearable accelerometers to characterize the effects of social isolation and/or loneliness experienced by adolescents during the COVID-19 pandemic on their mental and physical health.Methods We recruited 19 participants ages 13–18 from an Adolescent Medicine practice in Atlanta, Georgia. Participants completed surveys at baseline regarding their degree of social isolation and loneliness, as well as their family functioning, school climate, social media use, and COVID-19 experiences. Participants reported on their social isolation, loneliness, social media use, and emotional state up to four times daily for the subsequent 2-week study period using EMA. We also requested participants wear an activity tracker and heart rate measurement device for 14 days to monitor their physical health. Participant feedback was collected via open-ended exit interviews. Feasibility of recruitment/retention, adherence, and exploratory outcome measures were investigated. The pre-specified progression criteria were a recruitment rate of > 30% and a retention rate of > 80%.Results Participants’ ages ranged from 14 to 18 years, and most (13 of 19) identified as female and as Black/African American (16 of 19). Progression criteria were met for recruitment of participants, but not retention. Only 6 participants returned the wearable devices. On average, participants contributed 12 days of EMA data and 8 days of activity tracker/heart rate data. In exploratory analyses, social isolation was correlated with lower school climate, higher COVID-19 experiences, higher depression scores, and lower sleep quality. Loneliness also showed correlation with all these factors except COVID-19 experiences.Conclusions Retention in a cohort study using EMA and wearable accelerometer use was not feasible in this population of adolescents recruited during the COVID-19 pandemic. Baseline survey data did reveal important correlates of social isolation and loneliness that should be explored in future studies. Future research should further partner with adolescents to design longitudinal studies that mitigate barriers related to the pandemic and other factors on subject recruitment and retention.
A Novel c-Met/TRK inhibitor 1D228 efficiently inhibits tumor growth by targeting angiogenesis and tumor cell proliferation.
Authors: An Baijiao , Nie Wenyan , Hu Jinhui , Fan Yangyang , Yao Han , Ren Yuanyuan , Zhao Yaxuan , Nie Haoran , Wang Mengxuan , Zhang Chuanchuan , Wei Mengna , Li Wei , Liu Jiadai , Yang Chunhua , Zhang Yin , li xingshu , Tian Geng 
Published: May 2023

Abstract
It has been reported that c-Met and TRK synergistically promote multiple tumour progression, and therefore blocking the cross-signalling pathway between them may inhibit the growth of multiple tumours. In this study, we developed a tyrosine kinase inhibitor 1D228, which exhibited great anti-tumor activity by targeting TRK and c-Met. In the in vitro models, 1D228 showed a significant better inhibition on cancer cell proliferation and migration than the similar drug tepotinib. In the in vivo tumor models, 1D228 showed robust anti-tumor effect on gastric (TGI, 1D228-8 mg/kg/d: 94.8%; tepotinib 8mg/kg/d: 67.61%) and liver (TGI, 1D228-4 mg/kg/d: 93.4%; tepotinib 4mg/kg/d: 63.9%) tumor growth. Importantly, compared with the combination of larotrectinib and tepotinib, 1D228 monotherapy showed stronger antitumor activity and lower toxicity. Mechanistic studies showed that 1D228 can largely inhibit the phosphorylation of TRK and c-Met, thereby blocking downstream signaling pathways of TRK and c-Met. Interestingly, both kinases can be co-expressed at high levels in patients with gastric cancer. Cell cycle analysis found that 1D228 induced G0/G1 arrest by inhibiting cyclin D1. In addition, vascular endothelial cells also showed a pronounced response to 1D228 due to its expression of TRKB and c-Met. 1D228 suppressed the migration and tube formation of endothelial cells, which are the key functions of tumor angiogenesis. By histology analysis from the in vivo tumor tissues, we confirmed that 1D228 induced cancer cell apoptosis and inhibited tumor angiogenesis, leading to tumor growth retardation. Taken together, compound 1D228 may be a promising candidate for the next generation of c-Met and TRK inhibitors for cancer treatment, and offers a novel potential treatment strategy for gastric and hepatocellular carcinoma.
Transfusion practice in Central Norway – a regional cohort study in patients suffering from major haemorrhage
Authors: Carlsen Marte Irene Skille , Brede Jostein Rødseth , Medby Christian , Uleberg Oddvar 
Published: May 2023

Abstract
Background
In patients with major hemorrhage, balanced transfusions and limited crystalloid use is recommended in both civilian and military guidelines. This transfusion strategy is often used in the non-trauma patient despite lack of supporting data. Aim of the study was to describe the current transfusion practice in patients with major hemorrhage of both traumatic and non-traumatic etiology in Central Norway and discuss if transfusions are in accordance with appropriate massive transfusion protocols.
Methods
In this retrospective observational cohort study, data from four hospitals in Central Norway was collected from 01.01.2017 to 31.12.2018. All adults (≥18 years) receiving massive transfusion (MT) and alive on admission were included. MT was defined as a transfusion of ≥ 10 units of packed red blood cells (PRBC) within 24 hours or ≥ 5 units of PRBC during the first three hours after admission to hospital. Clinical data were collected from the hospital blood bank registry (ProSang) and electronic patient charts (CareSuite PICIS). Patients undergoing cardiothoracic surgery or extra corporeal membrane oxygenation treatment were excluded.
Results
A total of 174 patients were included in the study, of which 85.1% were non-trauma patients. Three quarters of all patients received plasma:PRBC in a ratio ≥ 1:2 (good practice), with no difference between the trauma and the non-trauma group (p=0.58). For the ratio of platelets:PRBC, 59.2% were transfused with ≥ 1:2 (good practice). There was no difference in blood transfusions between the trauma and non-trauma group (2273 mL versus 2309 mL, p=0.898). Mean fluid infusion of crystalloids in all patients were 6350 mL, with no difference between trauma and non-trauma group (p=0.137). Thirty-seven per cent of all patients received tranexamic acid, 53.4 % received calcium and fibrinogen was administered in 9.2%.
Conclusions
Most patients with major hemorrhage in Central Norway have a non-traumatic origin. Most patients are transfused with a high ratio of 1:2 plasma: PRBC and platelet:PRBC which is considered good practice. Crystalloids were administered liberally and there was a general underuse of adjunct medications such as tranexamic acid, calcium and fibrinogen.
Maximum extraction force of a cement-reinforced transsacral-2-sacral-wing screw
Authors: TONG Xinzhou , CHEN Lianglong , CHEN Yikai , ZHENG Yang , KANG Zhongshan , YANG Jinghai , LIU Jicheng , PENG Pan 
Published: May 2023

Abstract

Purpose To measure the maximum extraction force of cement-reinforced transsacral-2-sacral-wing screw fixation and to investigate the effectiveness of this internal fixation method.Methods Five adult cadaver specimens fixed with formaldehyde solution and moistened, as well as six fresh frozen cadavers, were utilized for this study. Three types of sacral screws were randomly placed on both sides of the same cadaveric specimen, including: (1) trans-sacral 2 sacral wing cement-reinforced screws (group S2); (2) S1-pedicle screws (group S1); and (3) sacral 2 sacroiliac screws (group S2AI). The immediate maximum extraction force values of the sacral screws in the three groups were recorded by applying axial extraction force to the screws. Statistical analysis was performed using ANOVA in SPSS 19.0, and statistical significance was set at P < 0.05. These findings provide insights into the biomechanical properties of sacral screws and could have implications for clinical applications.Results The maximum axial extraction force of the screws in group S2 was (521.80 ± 98.98) N; in group S1 was (843.16 ± 107.64) N; and in group S2AI was (536.04 ± 145.78) N. The results revealed that the maximum axial extraction force in group S1 was significantly stronger than that in groups S2 and S2AI (P < 0.001). In contrast, the maximum axial extraction force in groups S2 and S2AI was not significantly different (P > 0.05). Furthermore, in formaldehyde-fixed specimens, the maximum axial extraction force was significantly stronger in the S1 group than in the S2 and S2AI groups (P < 0.001), and in the S2 group than in the S2AI group (P < 0.05). Similar results were obtained in freshly frozen cadaveric specimens, where the maximum axial extraction force in group S1 was significantly stronger than that in groups S2 and S2AI (P < 0.001), and there was no significant difference between groups S2 and S2AI (P > 0.05).Conclusion In formaldehyde-fixed cadaveric specimens, the maximum axial extraction force of cement-reinforced transsacral-2-sacral-wing screw fixation was lower than that of S1-pedicle screw fixation, but higher than that of sacral 2 sacroiliac screw fixation. However, in freshly frozen cadaveric specimens, the difference in maximum extraction force between the S2 and S2AI groups was not statistically significant. Biomechanical experiments demonstrated that cement-reinforced transsacral-2-sacral-wing screws exhibit a significant effect on anti-pullout force performance, and may offer strong distal fixation. These screws may be an alternative to provide an effective force for spinal pelvic fixation.
Charge Stripes in the Graphene-based Materials
Authors: Radic Danko , Grozic Petra , Keran Barbara , Kadigrobov Anatoly 
Published: May 2023

Abstract
We present an analytical model of the charge density wave instability in graphene sheets within the intercalated graphite CaC6 compound. The instability yields the experimentally observed uniaxial charge stripes of periodically modulated electron density, coupled to the softest phonon mode of the superlattice consisting of the Ca atoms intercalated between graphene planes. The Fermi surface of the chemically
doped graphene undergoes the novel type of instability driven
by the mechanism that gains the condensation energy of the stripe state by the topological reconstruction of the Fermi surface. This mechanism appears to be entirely different from the one based on the Fermi surface nesting, which has been considered a paradigm in the present literature concerning the onset of charge density waves.
Studies on the Effect of Process Parameter on Corrosion behaviour of Electron Beam Welded Ti-based Alloy (Ti6Al4V)
Authors: Singh Jeetendra Kumar , Roy Gour Gopal , Majumdar Jyotsna Dutta 
Published: May 2023

Abstract

The present work concerns understanding the effect of process parameters on the corrosion behavior (in a 3.56 wt.% NaCl Solution) of electron beam welded Ti6Al4V developed with an acceleration voltage of 60 kV, beam current varying from 42–48 mA, and welding speed ranging from 800–1000 mm/min. In addition, beam oscillation (1–2 mm) was employed for the samples processed with an applied voltage of 900 mm/min. and beam current of 45 mA. Electron beam welding leads to the formation of defect-free welding with the presence of α, α' martensite, and a few β phases in the microstructure. A detailed electron backscattered diffraction study shows that the area fractions of low/high angle grain boundaries vary with process parameters. The average Kernal average misorientation angle of the electron beam welded samples varies from 0.49 ̊ − 0.57 ̊ as compared to 0.40 ̊ for as-received Ti6Al4V. Potentiodynamic polarization study in a 3.5 wt.% NaCl solution shows a superior corrosion resistance for the samples welded with beam oscillation as compared to the case without any oscillation. Electrochemical impedance spectroscopy measurement reveals the formation of a complex passivating film on the oxide surface. The X-ray photoelectron spectroscopy (XPS) analysis of the post-corroded samples shows the presence of titanium oxide (rutile and anatase) on the surface in the case of electron beam welded samples as compared to only anatase in as-received Ti6Al4V.
Predictive value of local control by 4'-[methyl-11C]-thiotymidine PET volume parameters in p16-negative oropharyngeal, hypopharyngeal, and supraglottic squamous cell carcinoma
Authors: Ouchi Yohei , Kishino Takehito , Miyashita Takenori , Mori Terushige , Mitamura Katsuya , Norikane Takashi , Yamamoto Yuka , Hoshikawa Hiroshi 
Published: May 2023

Abstract
Purpose: We investigated the potential of baseline 4′-[methyl-11C]-thiothymidine ([11C]4DST) PET for predicting loco-regional control of head and neck squamous cell carcinoma (HNSCC).
Methods: A retrospective analysis was performed using volumetric parameters, such as SUVmax, proliferative tumor volume (PTV), and total lesion proliferation (TLP), of pretreatment [11C]4DST PET for 91 patients with HNSCC with primary lesions in the oral cavity, hypopharynx, supraglottis, and oropharynx, which included p16-negative patients. PTV and TLP were calculated for primary lesions and metastatic lymph nodes combined. We examined the association among the parameters and relapse-free survival and whether case selection focused on biological characteristics improved the accuracy of prognosis prediction.
Results: The area under the curves (AUCs) using PTV and TLP were high for the oropharyngeal/hypopharyngeal/supraglottis groups (0.91 and 0.87, respectively), whereas that of SUVmax was 0.66 (p < 0.01). On the other hand, the oral group had lower AUCs for PTV and TLP (0.72 and 0.77, respectively). When all cases were examined, the AUCs using PTV and TLP were 0.84 and 0.83, respectively.
Conclusion: Baseline [11C]4DST PET/CT volume-based parameters can provide important prognostic information with p16-negative oropharyngeal, hypopharyngeal, and supraglottic cancer patients.
Loss of the cyclin kinase inhibitor p27kip1 opens a
therapeutic window by deregulating pathway choice
after DNA double strand breaks
Authors: Malek Nisar , Bozko Przemyslaw , Bui Khac Cuong , Rahbari Mohammad , Mattern Sven , Missios Pavlos , Sulyok Mihály , Garcia-Beccaria Maria , Fernandez Mirian , Roessler Stephanie , Goeppert Benjamin , Götze Julian , Scholta Tim , Reuter Sebastian , Nguyen Thi Mai Ly , Toulany Mahmoud , Shi Ying , Riebold Mathias , Rudalska Ramona , Dauch Daniel , Zender Lars , Schirmacher Peter , Zips Daniel , Singer Stephan , Heikenwaelder Mattias 
Published: May 2023

Abstract

Reduced expression of the cyclin kinase inhibitor p27kip1 is found in many human tumors and correlates with inferior prognosis. We tested the importance of p27 loss for the induction of genetic instability in Notch driven cholangiocarcinoma (CCA). Specifically, we asked how loss of p27 interferes with DNA repair pathway choice i.e. non-homologous-end-joining (NHEJ) or homologous recombination (HR). We detected all hallmarks of HR, in p27 deficient cells even though these cells had not undergone DNA replication. This defect in pathway choice depends on a previously unknown interaction of p27 with the RAD17 protein. In a cholangiocarcinoma mouse model loss of p27 greatly accelerated tumor formation but also resulted in a strikingly increased sensitivity against DNA damage response (DDR) targeting agents. We find that 30% of human CCC patients cluster in a group which corresponds to the DDR sensitive phenotype we have identified. In this work we show that the cyclin kinase inhibitor p27kip1 has an essential role in regulating the DNA damage response. This new activity of p27 is necessary to suppress homologous recombination-based DNA repair during the G1 phase. These findings point to a role of DDR targeting drugs in CCA with low levels of p27.
Bacterial communities in the potato tuberosphere share similarities with bulk soil and rhizosphere communities, yet possess distinct features
Authors: Voulgari Georgia , Schmalenberger Achim 
Published: May 2023

Abstract
Background and Aims
Bacterial communities in tuberospheres (soil attached to potato tubers) and levels of bacterial sulfur cycling in this compartment have been overlooked in healthy potato plants to date. Here, we aimed to describe the structure and composition of tuberosphere bacteriomes and its sulfur cycling capability using the rhizosphere and bulk soil bacterial community characteristics as a reference.
Methods
We collected rhizosphere and tuberosphere soil along with bulk soil from two field grown potato varieties with different properties. Bacterial communities were characterized by 16S rRNA gene amplicon sequencing. Bacterial sulfur cycling indicators were evaluated with cultivation dependent and independent methods and were correlated (Spearman) with the relative abundance of bacterial families.
Results
The structure of tuberosphere bacterial communities either overlapped with the bulk soil or had similarities with the rhizosphere. Relative abundance of specific bacterial families increased or decreased from bulk soil to tuberosphere and rhizosphere continuum. Tuberospheres had a tendency for higher arylsulfonate utilization compared to bulk soil. The families Sphindomonadaceae, Sphingobacteriaceae and Rhizobiaceae which presented a decline in their relative abundances from the rhizosphere to tuberosphere and bulk soil had positive correlations with sulfur cycling indicators.
Conclusion
Potato variety and soil characteristics played a role in structuring the tuberosphere bacterial communities. Tuberospheres represent a transitional environment between bulk soil and rhizosphere indicative from the intermediate relative abundances of specific bacterial families. A moderate stimulation of bacterial sulfur cycling activity in tuberospheres suggests that this microbial function may serve specific biological roles for potato tubers.
Molecular evolution of dengue virus: Bayesian approach considering 1,581 whole genome sequences from January 1944 to July 2022
Authors: Wolf Jonas , de Souza Ana Paula , de Schardosim Raine Fogliati , Pille Arthur , Maccari Juçara , Mutlaq Mohamed , Nasi Luiz Antonio 
Published: May 2023

Abstract
Dengue is a viral disease transmitted by mosquitoes that has spread rapidly across all continents in recent years. There are four distinct but closely related serotypes of the virus that causes dengue (DENV-1, DENV-2, DENV-3, and DENV-4). The present study evaluated dengue virus (DENV) serotypes' temporal spreading and molecular evolution worldwide. Bayesian coalescent analyses with was performed to study viral evolution. The results demonstrated that the tMRCA of DENV-1 was 1884-11-15 in Southeast Asia, DENV-2 was 1723-01-29 in Europe, DENV-3 was 1921-04-12 in Southeast Asia, and DENV-4 was 1876-03-28 in Southeast Asia. The origin of the DENV was in Spain in 1682, later it was disseminated in Asia and Oceania in 1847. After this period, the virus presented dissemination in North America in 1890. In South America, it was first disseminated to Ecuador in 1897 and then to Brazil in 1910. The dengue disease has had a significant impact on global health worldwide and the present study provides an overview of the molecular evolution of DENV serotypes.
A Garter Snake Optimization Algorithm for Constrained Optimization
Authors: Naghdiani Maryam , Jahanshahi Mohsen , Matin Reza Kazemi 
Published: May 2023

Abstract
The utilization of meta-heuristics has been widespread in resolving optimization problems, with constant development of new and effective algorithms. Thisresearch presents the Garter Snake Optimization Algorithm (GSO), which ismotivated by the mating behavior of garter snakes and leverages various techniques such as screening, grafting, and annual growth to conduct a productive andthorough search of the exploration space. The algorithm incorporates both socialand personal behaviors to accomplish a balance between exploration and exploitation of the best solutions. To assess the performance of the proposed algorithm,it is tested on four restricted benchmark problems and a frequently utilized realengineering problem, and the optimization results are compared with other algorithms. In many respects, the GSO outperforms other algorithms, demonstratingits superiority and potential in addressing constrained optimization problems.
S-doped C@MoSe2(1-x)S2x@MWCNT heterostructure as high-rate and long-cycle stability anodes for Na+ batteries
Authors: Yang Yincai , Wang Feng , Chen Youwen , Zhang Shiguo , Zhang Ming , Yu Zhen-Qiang , Au Chak-Tong , Yin Shuang-Feng , Qiu Renhua 
Published: May 2023

Abstract
Herein, we successfully synthesized C@MoSe2(1-x)S2x@MWCNT nanocomposite with heterojunctions as long-term stability anodes for sodium ion batteries (SIB). According to DFT calculations, the barrier of Na+ migration between MoSe2(1-x)S2x interlayers and that between GR@MoSe2(1-x)S2x@SWWCNT interlayers is 0.48 eV and 0.45 eV, respectively, significantly lower than that between MoSe2 interlayers (0.91 eV). Therefore, the introduction of S can enhance Na+ transport. The Se element can provide a larger interlayer spacing of MoSe2(1−x)S2x than MoS2, and MWCNT with high conductivity and thermal stability can be used as effective carriers for charge transfer. As an electrode material, PEG-200-2-C@MoSe2(1-x)S2x@MWCNT showed good performance for SIB: 300 mA h g-1 (500 cycles), 256 mA h g-1 (1000 cycles), 165 mA h g-1 (3000 cycles) and 126 mA h g-1 (6000 cycles) under the current density of 10 A g-1.
Comprehensive Analysis of Cuproptosis-related Genes in Prognosis and Tumor Microenvironment Infiltration in Colorectal Cancer
Authors: Chen Weiyi , Hu Ke , Liu Yu , Li Xiaocheng , Peng Jing , Chen Lijun , Duan Shaoyi , Yang Qizhang 
Published: May 2023

Abstract
Colorectal cancer (CRC) is a common malignancy, with high incidence and mortality rates. Cuproptosis, a novel form of copper-induced programmed cell death, contributes to tumor progression. Whether cuproptosis-related genes (CRGs) play a role in CRC remains unclear. Here, we systematically assessed CRG mutations in CRC. Seven of the 10 CRGs exhibited significant differential expression between CRC and normal tissues. CDKN2A and DLAT were identified as independent risk factors for predicting overall survival (OS) in CRC using univariate and multivariate Cox analyses, and a risk score model was constructed based on CDKN2A and DLAT. Patients with CRC were categorized into high- and low-risk groups based on their median risk scores. Patients with CRC in the low-risk group had longer OS than those in the high-risk group. Receiver operating characteristic curve analysis indicated that the risk score model had good predictive accuracy for OS. Spearman’s analysis showed that CDKN2A and DLAT expressions were significantly associated with immune cell infiltration and immune checkpoints in CRC. Our research revealed CRGs’ prognostic value, particularly CDKN2A and DLAT, in CRC and demonstrated the relationship between CDKN2A/DLAT and immune infiltration in CRC, thereby contributing to the outcome evaluation of patients with CRC and identifying novel targets for CRC immunotherapy.