bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient portal interventions: a scoping review of functionality, automation used, and therapeutic elements of patient portal interventions
Authors: Gleason Kelly T , Powell Danielle S , Wec Aleksandra , Zou Xingyuan , Gamper Mary Jo , Peereboom Danielle , Wolff Jennifer L 
Published: August 2023

AbstractObjectivesWe sought to understand the objectives, targeted populations, therapeutic elements, and delivery characteristics of patient portal interventions.Materials and MethodsFollowing Arksey and O-Malley’s methodological framework, we conducted a scoping review of manuscripts published through June 2022 by hand and systematically searching PubMed, PSYCHInfo, Embase, and Web of Science. The search yielded 5403 manuscripts; 248 were selected for full-text review; 81 met the eligibility criteria for examining outcomes of a patient portal intervention.ResultsThe 81 articles described: trials involving comparison groups (n = 37; 45.7%), quality improvement initiatives (n = 15; 18.5%), pilot studies (n = 7; 8.6%), and single-arm studies (n = 22; 27.2%). Studies were conducted in primary care (n = 33, 40.7%), specialty outpatient (n = 24, 29.6%), or inpatient settings (n = 4, 4.9%)—or they were deployed system wide (n = 9, 11.1%). Interventions targeted specific health conditions (n = 35, 43.2%), promoted preventive services (n = 19, 23.5%), or addressed communication (n = 19, 23.4%); few specifically sought to improve the patient experience (n = 3, 3.7%). About half of the studies (n = 40, 49.4%) relied on human involvement, and about half involved personalized (vs exclusively standardized) elements (n = 42, 51.8%). Interventions commonly collected patient-reported information (n = 36, 44.4%), provided education (n = 35, 43.2%), or deployed preventive service reminders (n = 14, 17.3%).DiscussionThis scoping review finds that most patient portal interventions have delivered education or facilitated collection of patient-reported information. Few interventions have involved pragmatic designs or been deployed system wide.ConclusionThe patient portal is an important tool in real-world efforts to more effectively support patients, but interventions to date rely largely on evidence from consented participants rather than pragmatically implemented systems-level initiatives.
Parasitism by Gryon aetherium (Hymenoptera: Scelionidae) on Bagrada hilaris (Hemiptera: Pentatomidae) eggs in northcentral California
Authors: Hogg Brian N , Grettenberger Ian M , Borkent Christopher J 
Published: August 2023

Abstract
Bagrada bug, Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae), is an invasive pest of cruciferous crops. The parasitoid Gryon aetherium Talamas (Hymenoptera: Scelionidae) is a promising biological control agent for B. hilaris because it can forage in the soil where B. hilaris deposits most of its eggs. In this study, we assessed parasitism by G. aetherium on B. hilaris eggs in situ in northcentral California, including the Salinas Valley where most cruciferous crops in the United States are grown. Parasitism was documented by leaving soil-filled trays under infested plants for 7–14 days, then removing eggs and holding them for emergence of parasitoids. Gryon aetherium accounted for over 99% of emerged parasitoids, and occurred at 11 of the 12 sampled sites. Of the 17,729 and 31,759 B. hilaris eggs collected in 2021 and 2022, 1,518 (8.84%) and 2,654 (8.36%) were parasitized by G. aetherium, respectively. Parasitism rates were generally higher inland and ranged from 3.64% to 44.93% in 2021 and from 1.01% to 23.04% in 2022, and never exceeded 15% on any sample dates at several coastal sites in the Salinas Valley. Discovery efficiency (a measure of the ability of parasitoids to locate egg patches) reached 80% or higher at all but 1 site, but exploitation efficiency (a measure of the ability of parasitoids to exploit the egg patch after it has been discovered) was generally &lt;20%, suggesting that G. aetherium can locate egg patches efficiently but is less efficient at finding eggs within patches.
Black issue publics online: securing political knowledge through selective exposure
Authors: Kleinberg Mona S 
Published: August 2023

Abstract
Political knowledge is fundamental to democratic politics. I develop a group-centered theory of political knowledge acquisition in the current media environment, which includes both high- and low-choice media, in this article. I argue that group identity prompts selective exposure to media content, which gives rise to specialized group-relevant political knowledge. This specialized knowledge is deeply relevant to the group and cannot be measured with indicators of general political knowledge. I show that selective exposure is the crucial mechanism facilitating specialized knowledge: Both selective exposure to Black-oriented content and use of high-choice media (the Internet) increase group-relevant knowledge among Black issue publics. This research speaks to scholarship examining the role of digital media in democratic politics and illustrates that the affordances of the Internet, and particularly selective exposure, are crucial to marginalized groups, who do not see their interests represented in mainstream media content, but who can access such information online.
Assessing the consistency of fact-checking in political debates
Authors: Lelo Thales 
Published: August 2023

Abstract
In the scholarly literature on journalism and political communication, there has been an expectation that fact-checkers would play an important role in ensuring democratic accountability, especially during pivotal political moments. This piece scrutinizes the level of agreement between five Brazilian fact-checking groups and the reasons for divergences in their verdicts during the presidential debates of the 2022 campaign. The emphasis is on claims checked by two or more organizations. Through a mixed-methods approach, it shows a widespread lack of consistency among fact-checkers, which is explained by their conflicting methods and interpretations of candidates’ words. This study adds to the existing scholarship by challenging the dominant framework on fact-checking, putting into question its democracy-building role in critical circumstances, as well as the epistemology it relies on to assess the veracity of political discourse. Complementary, it introduces a valuable methodology for studying the rationale underlying fact-checking ratings.
Widespread effects of DNA methylation and intra-motif dependencies revealed by novel transcription factor binding models
Authors: Grau Jan , Schmidt Florian , Schulz Marcel H 
Published: August 2023

Abstract
Several studies suggested that transcription factor (TF) binding to DNA may be impaired or enhanced by DNA methylation. We present MeDeMo, a toolbox for TF motif analysis that combines information about DNA methylation with models capturing intra-motif dependencies. In a large-scale study using ChIP-seq data for 335 TFs, we identify novel TFs that show a binding behaviour associated with DNA methylation. Overall, we find that the presence of CpG methylation decreases the likelihood of binding for the majority of methylation-associated TFs. For a considerable subset of TFs, we show that intra-motif dependencies are pivotal for accurately modelling the impact of DNA methylation on TF binding. We illustrate that the novel methylation-aware TF binding models allow to predict differential ChIP-seq peaks and improve the genome-wide analysis of TF binding. Our work indicates that simplistic models that neglect the effect of DNA methylation on DNA binding may lead to systematic underperformance for methylation-associated TFs.
An archaeal transcription factor EnfR with a novel ‘eighth note’ fold controls hydrogen production of a hyperthermophilic archaeon Thermococcus onnurineus NA1
Authors: Bae Da-Woon , Lee Seong Hyuk , Park Ji Hye , Son Se-Young , Lin Yuxi , Lee Jung Hyen , Jang Bo-Ram , Lee Kyu-Ho , Lee Young-Ho , Lee Hyun Sook , Kang Sung Gyun , Kim Byoung Sik , Cha Sun-Shin 
Published: August 2023

Abstract
Thermococcus onnurineus NA1, a hyperthermophilic carboxydotrophic archaeon, produces H2 through CO oxidation catalyzed by proteins encoded in a carbon monoxide dehydrogenase (CODH) gene cluster. TON_1525 with a DNA-binding helix-turn-helix (HTH) motif is a putative repressor regulating the transcriptional expression of the codh gene cluster. The T55I mutation in TON_1525 led to enhanced H2 production accompanied by the increased expression of genes in the codh cluster. Here, TON_1525 was demonstrated to be a dimer. Monomeric TON_1525 adopts a novel ‘eighth note’ symbol-like fold (referred to as ‘eighth note’ fold regulator, EnfR), and the dimerization mode of EnfR is unique in that it has no resemblance to structures in the Protein Data Bank. According to footprinting and gel shift assays, dimeric EnfR binds to a 36-bp pseudo-palindromic inverted repeat in the promoter region of the codh gene cluster, which is supported by an in silico EnfR/DNA complex model and mutational studies revealing the implication of N-terminal loops as well as HTH motifs in DNA recognition. The DNA-binding affinity of the T55I mutant was lowered by ∼15-fold, for which the conformational change of N-terminal loops is responsible. In addition, transcriptome analysis suggested that EnfR could regulate diverse metabolic processes besides H2 production.
Association between police officers’ stress and perceived health
Authors: Kukić Filip , Streetman Aspen , Heinrich Katie M , Popović-Mančević Marija , Koropanovski Nenad 
Published: August 2023

Abstract
Police experience occupational stress in two categories: operational (i.e. long hours, shift work, crime scenes) and organizational (e.g. favouritism, bureaucratic red tape) stress. High occupational stress may harm officers’ health perceptions, decreasing individual policing capacity. This study surveyed the relationship between operational and organizational stress and perceived health among 1,220 Serbian police officers (female = 232 [19%]; mean age 39.1 ± 8.4 years). Participants answered a single general health question, a 20-question operational police stress questionnaire, and a 20-question organizational police stress questionnaire. Perceived health was negatively associated with operational and organizational stress. Moderate negative associations were found between age and health and obesity status and health. When age groups were analysed separately, older officers with good or great health reported lower operational and organizational stress levels. These results suggest that better-perceived health may be protective against stress for police officers.
Phenotypic and genotypic characterization of Chinese adult patients with NLRP3-associated autoinflammatory disease with hearing loss
Authors: Wu Bingxuan , Luo Yi , Wu Di , Wang Yi , Shen Min 
Published: August 2023

Abstract

Objectives
Nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3-associated autoinflammatory disease (NLRP3-AID) is a rare autosomal dominant systemic autoinflammatory disease. We aimed to summarize the phenotypic and genotypic features of Chinese adult NLRP3-AID patients with hearing loss.

Methods
A retrospective cohort study of twenty-one adult patients with NLRP3-AID was conducted in Peking Union Medical College Hospital between July 2015 and March 2023. All patients underwent whole exome sequencing and otorhinolaryngologic assessments. Clinical features and therapeutic data were collected and analysed.

Results
We found that 13/21 (61.90%) of patients had hearing loss with high-frequency impairment in the majority, and most patients presented with vestibular dysfunction as a new finding. The NLRP3-AID patients with early-onset, cold or stress triggered episodes, red eyes, fatigue, hypopsia and mutations located in the NACHT domain of the NLRP3 protein were more likely to suffer from hearing loss, especially sensorineural hearing loss, perhaps as a result of pathogenic variants of high penetrance. By a series of audiological evaluations, TNF-α inhibitors were confirmed to improve or reverse hearing loss.

Conclusions
We reported the first cohort of Chinese adult NLRP3-AID patients with hearing loss and characterized vestibular dysfunction, highlighted the necessity for attention to high-frequency hearing and provided potential alternative treatment.
Hypomethylation of CD3D promoter induces immune cell infiltration and supports malignant phenotypes in uveal melanoma
Authors: Zhang Chao , Wu Shuai 
Published: August 2023

AbstractAlterations in DNA methylation in malignant diseases have been heralded as promising targets for diagnostic, prognostic, and predictive values. This study was based on epigenetic alterations and immune cell infiltration analysis to investigate the mechanism of CD3D methylation in uveal melanoma (UM). Bioinformatics analysis was performed on transcriptome data, 450 K methylation data, and clinical information of UM patients from the TCGA database. Stromal and immune cell infiltration was evaluated by calculating the StromalScore and ImmuneScore of UM samples. UM samples were divided into high and low StromalScore and ImmuneScore groups, followed by differential and enrichment analyses. PPI network construction and correlation analysis was used to identify the core prognosis‐related genes. The bioinformatics analysis results were confirmed in UM cell experiments. StromalScore and ImmuneScore were significantly associated with the prognosis of UM patients. CD3D, IRF1, CCL3, and FN1 were identified as core genes driven by methylation that affected the prognosis of UM patients. CD3D expression showed the highest correlation with its methylation and was closely related to the four key immune cells in UM development. CD3D was hypomethylated and abundantly expressed in UM cells, while silencing of CD3D inhibited the proliferation, migration, and invasion of UM cells in vitro. In summary, this study identifies hypomethylation of CD3D promoter in UM, which was associated with immune cell infiltration of UM.
Corrective Maneuvers Used in Different Scoliosis Deformities With the Experience of 72 Operated Patients
Authors: Bayram Yusuf , Polat Ömer , Karataş Muhammed E. , Günaydin Fatih , Kesebir Enes , Çaçan Mehmet A. , Uçar Bekir Y. 
Published: August 2023

Purpose:
This study aimed to show the correction maneuvers used in scoliosis surgery and give hints and tips on achieving optimal correction with the help of visual content.

Materials and Methods:
Seventy-two scoliosis patients with 96 major curves operated between 2011 and 2018 evaluated retrospectively. Seven different correction maneuvers (Ucar convex rod rotation technique, cantilever technique, convex double-rod rotation technique, convex double-rod translation technique, double-rod rotation with coronal bending, convex rod rotation with coronal bending, direct vertebral rotation) were used in these surgeries alone or together. Each method is explained with figures and videos. Type of scoliosis, follow-up time, age, preoperative and postoperative degree of curvature, amount of correction, surgical time, amount of transfusion, hemoglobin level, and hospital stay were evaluated.

Results:
The mean follow-up was 42.5 (24–108) months, and the mean age was 15.8 (12–29) years. The mean preoperative scoliosis angle of the patients was 75.9 (50–139) degrees, and the final follow-up was 15.6 (5–40) degrees. The scoliotic deformity correction rate was 79.3%. The mean preoperative thoracic kyphosis of the patients was 58.7 (12–110) degrees, and the final follow-up was 41.2 (25–62) degrees. The mean surgical time was 293 (160–440) minutes. The mean hospitalization length was 5.8 (2–21) days. The blood delivered to patients during surgery and postoperative was 715 (300–1800) mL. There was a statistically significant difference between the preoperative and final follow-up scoliosis angle (P=0.000).

Conclusions:
Providing an ideal correction without damaging the spinal cord depends on correctly applying the correction maneuvers. The use of correction maneuvers alone or in combination in the appropriate order will shorten the duration of the case, decrease the amount of bleeding, shorten the hospital stay, increase the amount of correction, and reduce complications.
Biomechanical Study of Horizontal Screw-screw Crosslink in C1–2 Pedicle Screw-rod Fixation
Authors: Ouyang Beiping , Zou Xiaobao , Luo Chunshan , Lu Tingsheng , Ma Xiangyang 
Published: August 2023

Study Design:
This is a biomechanical study in vitro.

Objective:
To investigate the biomechanical differences between horizontal rod-rod crosslink (hR-R CL) and the horizontal screw-screw crosslink (hS-S CL) implementation in C1–2 pedicle screw-rod (C1–2 PSR) fixation.

Summary of Background:
To improve internal fixation stability, transverse connector (TC) is used in C1-2 PSR to increase torsional stiffness. The connection mode of horizontal connection includes hR-R CL and hS-S CL. hS-S CL adopted in C1–2 PSR was rarely reported and its biomechanics are still unclear.

Materials and Methods:
Six fresh cadaveric cervical spine specimens were each tested as an Intact model, then modified and tested as an Instability model (unstable odontoid fractures), and then as 3 internal fixation models, including C1–2 PSR, C1–2 pedicle screw-rod+horizontal rod-rod crosslink (C1–2 PSR+ hR-R CL), C1–2 pedicle screw-rod+horizontal screw-screw crosslink (C1–2 PSR+ hS-S CL). The ROM of the C1–2 segments was measured by applying 1.5 nm load in 6 loading conditions, including flexion-extension (FE), left and right lateral bending (LB), and left and right axial rotation (AR).

Results:
The C1–2 PSR+hR-R CL and C1–2 PSR+hS-S CL models, respectively, showed 60% and 75% lower ROM than the C1–2 PSR model in LB and AR conditions (P<0.05). ROM was comparable between the C1–2 PSR+hR-R CL and the C1–2 PSR+hS-S CL models in all loading conditions (P>0.05).

Conclusion:
Both types of crosslinks showed superior C1–2 stability under LB and AR conditions than PSR without crosslinks. The C1–2 segment stability was comparable between the 2 types of crosslinks themselves.
Pilot Study on Percutaneous Delivery of Recombinant Human Bone Morphongenetic Protein-2 Augments Fusion in a Nicotine-impaired Rabbit Fusion Model
Authors: Virk Sohrab , Vaishnav Avani , Kumagai Hiroshi , Yao Yu-Cheng , Dowdell James , Sandhu Harvinder , Schwab Frank , Qureshi Sheeraz 
Published: August 2023

Study Design:
A nicotine-impaired spinal fusion rabbit model.

Objective:
To examine whether controlled delivery of morselized absorbable collagen sponge recombinant human bone morphogenetic protein-2 (rhBMP2) in a delayed manner postsurgery would allow for improved bone healing.

Summary of Background Data:
The current delivery method of rhBMP-2 during surgery causes a burst of rhBMP-2, which is not sustained. Given that bone morphogenetic protein 2 (BMP-2) expression peaks later in the fusion process, there may be the benefit of delivery of rhBMP-2 later in the healing process.

Methods:
Sixteen male 1-year-old rabbits underwent a posterolateral spinal fusion with iliac crest bone graft at L5–L6 while being given nicotine to prevent spinal fusion as previously published. Eight were controls, whereas 8 had morselized rhBMP-2 (4.2 mg) injected at the fusion site at 4 weeks postoperatively. Histologic, radiologic, and palpation examinations were performed at 12 weeks to determine fusion status and the volume of bone formed. Hematoxylin and eosin stains were used for histology. A Student t test was used to compare the computed tomography scan measured volume of bone created between the control cohort (CC) and rhBMP-2 delayed delivery cohort (BMP-DDC).

Results:
Of the total, 7/8 rabbits in the BMP-DDC and 5/8 rabbits in the CC formed definitive fusion with a positive palpation examination, bridging bone between transverse processes on computed tomography scan, and an x-ray showing fusion. Histologic analysis revealed newly remodeled bone within the BMP-DDC. There was an increased average volume of bone formed within the BMP-DDC versus the CC (22.6 ± 13.1 vs 11.1 ± 3.6 cm3, P = 0.04).

Conclusion:
Our study shows that injectable morselized absorbable collagen sponge/rhBMP-2 can create twice as much bone within a nicotine-impaired rabbit spine fusion model when delivered 4 weeks out from the time of surgery.
Telomere length is associated with increased risk of cutaneous melanoma: a Mendelian randomization study
Authors: Liu Mingjuan , Lan Yining , Zhang Hanlin , Zhang Xinyi , Wu Mengyin , Yang Leyan , Zhou Jia , Tong Meiyi , Leng Ling , Zheng Heyi , Li Jun , Mi Xia 
Published: August 2023

The objective was to investigate the causal association between genetically predicted telomere length (TL) and cutaneous melanoma (CM) risk using Mendelian randomization (MR). This study utilized a two-sample MR and two large genome-wide association studies (GWAS) were used for instrumental variable (IV) selection to select single nucleotide polymorphisms at the genome-wide significance threshold (P < 5E-08) for TL. The IVs were then pruned for linkage disequilibrium and weak instrument bias. Summary statistics from a GWAS meta-analysis of CM were used as the outcome variable. The inverse variance weighted (IVW) method was used as the primary approach for overall causal estimation in MR, with sensitivity analyses performed to assess robustness. All statistical analyses were conducted in R studio.

Results
The MR analysis using two TL GWAS datasets revealed strong and consistent evidence that long TL is causally associated with an increased risk of CM. The analysis of the Codd et al. dataset found that long TL significantly predicted an elevated risk of CM (IVW OR = 2.411, 95% CI 2.092–2.780, P = 8.05E-34). Similarly, the analysis of the Li et al. dataset yielded consistent positive results across all MR methods, providing further robustness to the causal relationship (IVW OR = 2.324, 95% CI 1.516–3.565, P = 1.11E-04). The study provides evidence for a causal association between TL and CM susceptibility, indicating that longer TL increases the risk of developing CM and providing insight into the unique telomere biology in melanoma pathogenesis. Telomere maintenance pathways may be a potential target for preventing and treating CM.
Advanced practice nursing in France: Social capital assessment
Authors: Brandman J. D. Wailua , Sato Randall M. 
Published: August 2023

ABSTRACT
France is in the initial stage of development of the advanced practice nursing role. Social capital is essential to move this development forward, as evidenced by similar global movements resulting in the establishment of the advanced practice role of nurses. A pilot project examined the general response of French citizens (social capital) to the idea of advanced nursing practice in their country. The overwhelming majority of participants were in support of advanced nursing practice, with 90% of the participants answering “Yes” to the question “Would you trust and feel safe having a Nurse Practitioner (APRN) provide your primary care instead of a physician?” Social capital is needed in many areas of health care and may be enhanced by strong social capital within the citizenry.