bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of Xpert MTB/RIF and sputum microscopy compared to sputum culture for diagnosis of tuberculosis in seven hospitals in Indonesia
Authors: Karuniawati Anis , Burhan Erlina , Koendhori Eko Budi , Sari Desvita , Haryanto Budi , Nuryastuti Titik , Gayatri A. A. A. Yuli , Bahrun Uleng , Kusumawati R. Lia , Sugiyono Retna Indah , Susanto Nugroho Harry , Diana Aly , Kosasih Herman , Naysilla Adhella Menur , Lokida Dewi , Neal Aaron , Siddiqui Sophia , Lau Chuen-Yen , Karyana Muhammad 
Published: January 2023

IntroductionTuberculosis (TB) is a major public health concern in Indonesia, where the incidence was 301 cases per 100,000 inhabitants in 2020 and the prevalence of multi-drug resistant (MDR) TB is increasing. Diagnostic testing approaches vary across Indonesia due to resource limitations. Acid-fast bacilli (AFB) smear is widely used, though Xpert MTB/RIF has been the preferred assay for detecting TB and rifampicin resistance since 2012 due to higher sensitivity and ability to rapidly identify rifampicin resistance. However, &lt;1,000 Xpert instruments were available in Indonesia as of 2020 and the Xpert supply chain has suffered interruptions.MethodsWe compared the performance of Xpert MTB/RIF and AFB smear to facilitate optimization of TB case identification. We analyzed baseline data from a cohort study of adults with pulmonary TB conducted at seven hospitals across Indonesia. We evaluated sensitivity and specificity of AFB smear and Xpert MTB/RIF using Mycobacterium tuberculosis (Mtb) culture as the gold standard, factors associated with assay results, and consistency of Xpert MTB/RIF with drug susceptibility test (DST) in detecting rifampicin resistance.ResultsSensitivity of AFB smear was significantly lower than Xpert MTB/RIF (86.2 vs. 97.4%, p-value &lt;0.001), but specificity was significantly better (86.7 vs. 73.3%, p-value &lt;0.001). Performance varied by hospital. Positivity rate for AFB smear and Mtb culture was higher in subjects with pulmonary cavities and in morning sputum samples. Consistency of Xpert MTB/RIF with DST was lower in those with rifampicin- sensitive TB by DST.DiscussionAdditional evaluation using sputa from primary and secondary Indonesian health centers will increase the generalizability of the assessment of AFB smear and Xpert MTB/RIF performance, and better inform health policy.Clinical trial registration[https://clinicaltrials.gov/], identifier [NCT027 58236].
Further analysis of natural antibodies against ischemic stroke
Authors: Qi Jingjing , Jiang Quanhang , Wang Peng , Wang Zhenqi , Zhang Xuan 
Published: January 2023

BackgroundOur previous study revealed that circulating levels of IgG natural antibodies (NAbs) for vascular endothelial growth factor receptor 1 (VEGFR1) were significantly decreased in patients with arteriosclerosis compared with control subjects. To enhance the sensitivity of an enzyme-linked immunosorbent assay (ELISA) developed in-house for antibody testing, the present work was designed to investigate additive signals in the in-house ELISA developed with the combination of two or more linear peptide antigens derived from target proteins of interest, including VEGFR1, oxidized low-density lipoprotein receptor 1 (LOX-1), interleukins 6 (IL6) and 8 (IL8).MethodsA total of 218 patients with ischemic stroke and 198 healthy controls were enrolled and an in-house ELISA was developed with linear peptides derived from VEGFR1, LOX-1, IL6, and IL8 to detect their IgG levels in plasma.ResultsCompared with control subjects, plasma levels of IgG NAbs for the IL6-IL8 combination were significantly lower in female patients (Z = −3.149, P = 0.002), whereas male patients showed significantly lower levels of plasma anti-VEGFR IgG (Z = −3.895, P &lt; 0.001) and anti-LOX1a IgG (Z = −4.329, P &lt; 0.001). Because plasma levels of IgG NAbs for both the IL6-IL8-LOX1a-LOX1b combination and the VEGFR1a-VEGFR1b-LOX1a-LOX1b combination were significantly lower in the patient group than the control group, receiver operating characteristic (ROC) analysis was performed and the results showed that the VEGFR1a-VEGFR1b-LOX1a-LOX1b combination had an area under the ROC curve (AUC) of 0.70 for its IgG assay with a sensitivity of 27.1% against the specificity of 95.5% and that the IL6-IL8-LOX1a-LOX1b combination had an AUC of 0.67 for its IgG assay with a sensitivity of 21.1% against the specificity of 95.5%. Spearman correlation analysis showed that plasma IgG NAbs against the IL6-IL8 combination were positively correlated with carotid plaque size only in male patients (r = 0.270, p = 0.002).ConclusionsCirculating IgG NAbs for the target molecules studied may be potential biomarkers for a subgroup of ischemic stroke and also contribute to the gender differences in clinical presentation of the disease.
Downregulation of ITGβ3 in colon adenocarcinoma reveals poor prognosis by affecting genome stability, cell cycle, and the tumor immune microenvironment
Authors: Zhao Lei , Ma Xiaoting , Li Guangxin , Zhao Pengfei , Lin Haishan , Ma Yingjie , Li Huihui , Yu Jing 
Published: January 2023

IntroductionAbnormal expression of integrin subunit beta 3 (ITGβ3), a gene-encoding protein, is related to the occurrence and development of cancers; however, the biological role of ITGβ3 in colon adenocarcinoma (COAD) remains unclear.MethodsWe used the Cancer Genome Atlas database to obtain the clinical data of patients with COAD, analyzed the mRNA gene clusters related to ITGβ3, and analyzed the interaction signal pathway and interaction protein network of the differentially expressed gene clusters. The results showed that ITGβ3 expression in COAD tumor tissues was significantly downregulated compared with that in paracancerous tissues. Low ITGβ3 expression in tumor tissues is associated with poor overall survival of patients with COAD. In multivariate analysis, stage IV and ITGβ3 low expression were independent prognostic factors. Gene Ontology analysis showed that differentially expressed genes (DEGs) were significantly enriched in leukocyte migration, cell adhesion, and extracellular matrix (ECM) organization. Kyoto Encyclopedia of Genes and Genomes analysis revealed that the DEGs were mainly enriched in ECM-receptor interactions, focal adhesion, and the PI3K-Akt signaling pathway. Protein-protein interaction network analysis revealed the hub and seed genes of the key modules related to ITGβ3. Finally, we analyzed the correlation between TGβ3 and immune-related genes and found that ITGβ3 expression was significantly correlated with tumor purity and infiltration level of dominant immune cells.DiscussionThese findings indicate that ITGβ3 downregulation in COAD may profoundly affect genome stability and multiple steps of the cell cycle, alter the tumor immune microenvironment, and be related to the prognosis of patients with COAD.
Long-term outcomes of nasopharyngeal carcinoma treated with helical tomotherapy using simultaneous integrated boost technique: A 10-year result
Authors: Meng Lingling , Teng Feng , Liu Qiteng , Du Lei , Cai Boning , Xie Chuanbin , Gong Hanshun , Zhang Xinxin , Ma Lin 
Published: January 2023

BackgroundTo evaluate the long-term survival and treatment-related toxicities of helical tomotherapy (HT) in nasopharyngeal carcinoma (NPC) patients.MethodsOne hundred and ninety newly diagnosed non-metastatic NPC patients treated with HT from September 2007 to August 2012 were analyzed retrospectively. The dose at D95 prescribed was 70-74Gy, 60-62.7Gy and 52-56Gy delivered in 33 fractions to the primary gross tumor volume (pGTVnx) and positive lymph nodes (pGTVnd), the high risk planning target volume (PTV1), and the low risk planning target volume (PTV2), respectively, using simultaneous integrated boost technique. The statistical analyses were performed and late toxicities were evaluated and scored according to the Common Terminology Criteria for Adverse Events (version 3.0).ResultsThe median follow-up time was 145 months. The 10-year local relapse-free survival (LRFS), nodal relapse-free survival (NRFS), distant metastasis-free survival (DMFS) and overall survival (OS) were 94%, 95%, 86%, and 77.8%; respectively. Fifty (26.3%) patients had treatment-related failures at the last follow-up visit. Distant metastasis, occurred in 25 patients, was the major failure pattern. Multivariate analysis showed that age and T stage were independent predictors of DMFS and OS, Concomitant chemotherapy improved overall survival, but anti-EGFR monoclonal antibody therapy failed. The most common late toxicities were mainly graded as 1 or 2.ConclusionsHelical tomotherapy with simultaneous integrated boost technique offered excellent long-term outcomes for NPC patients, with mild late treatment-related toxicities. Age and clinical stage were independent predictors of DMFS and OS. And, concurrent chemotherapy means better OS. Further prospective study is needed to confirm the superiority of this technology and to evaluate the roles of anti-EGFR monoclonal antibody treatment.
Inhibitory activity of Enhydra fluctuans Lour. on calcium oxalate crystallisation through in silico and in vitro studies
Authors: Chattaraj Bornika , Nandi Arijit , Das Anwesha , Sharma Amit , Dey Yadu Nandan , Kumar Dharmendra , R Mogana 
Published: January 2023

The decoction of the whole plant of Enhydra fluctuans is used ethno medicinally by various tribes for the treatment of kidney stones and urinary problems. However, no scientific studies were carried out to delineate its influence on urinary stone formation and crystallisation. Hence, the present study is proposed to investigate the effect of the aqueous extract of Enhydra fluctuans extract on in vitro crystallisation of calcium oxalate. The present study also evaluated. in silico studies of the metabolites with the target proteins present in the renal calcium oxalate stone matrix. The plant material was subjected to decoction to obtain an aqueous extract. The effect of the extract on calcium oxalate crystallization was evaluated by in vitro nucleation and aggregation assays. Further, the metabolites present in E. fluctuans were mined from the existing literature and their number was found to be 35. The selected 35 metabolites of E. fluctuans were subjected to molecular docking with the 5 proteins which are known to be responsible for calcium oxalate crystal growth. Results of in vitro studies indicated that the extract (50, 100, and 200 μg/mL) and standard drug cystone (1,000 μg/mL) exhibited an inhibitory role in the nucleation process where the percentage inhibitions were 52.69, 43.47, 21.98, and 31.67 μg/mL respectively. The results of molecular docking studies revealed that 2 out of 35 metabolites i.e. Baicalein-7-O-diglucoside and 4′,5,6,7-Tetrahydroxy-8-methoxy isoflavone-7-O-beta-D- galactopyranosyl-(1→3)-O-beta-D-xylopyranosyl-(1→4)- O-alpha-L-rhamnopyranoside showed modulatory effects on the four renal stone matrix-associated protein (Human CTP: Phosphoethanolamine Cytidylyltransferase (Protein Data Bank ID: 3ELB), UDP glucose: glycoprotein glucosyltransferase 2 (Gene: UGGT2) (AlphaFold) and RIMS-binding protein 3A (Gene: RIMBP3) (AlphaFold), and Ras GTPase activating-like protein (PDB: 3FAY) based on their docking scores which indicates that they may inhibit the crystallization process. Findings from this study show that Enhydra fluctuans may be effective in the prevention of the crystallization of calcium oxalate. However, further, in vivo studies as well as molecular studies are needed to be conducted to confirm and strengthen its anti-urolithiatic activity and to elucidate the possible mechanism of action involved therein.
Long survival in a pancreatic carcinoma patient with multi-organ toxicities after sintilimab treatment: A case report
Authors: Ni Chen-Xu , Zhao Yu , Qian Hong , Fu Hui , Yan Yu-Ying , Qiu Yu-Shuang , Zhou Can-Can , Huang Fang , Shen Fu-Ming , Li Dong-Jie , Xu Qing 
Published: January 2023

Pancreatic carcinoma is the leading cause of death among digestive malignancies in China. In particular, there is no breakthrough in prolonging the survival of pancreatic cancer patients with chemical and targeted therapies. Tumor immunotherapy brings opportunities and progress for the treatment of pancreatic cancer. Sintilimab is an innovative PD-1 inhibitor which was reported certain clinical benefits in multi-line treatments of advanced pancreatic cancer with gemcitabine. The combination therapy of PD-1 with gemcitabine plus high-intensity focused ultrasound (HIFU) in pancreatic cancer has not been reported. Here we report a case of a Chinese old patient diagnosed with metastatic pancreatic cancer. Two months after sintilimab treatment, the patient occurred severe immune colitis. The patient was diagnosed with immune ureteritis after 8 months of treatment. The immue-related adverse events (irAEs) refined after timely recognition and correct intervention by the clinician and clinical pharmacist. After first-line treatment of sintilimab plus gemcitabine combined with pancreatic HIFU, the patient achieved a remarkable benefit of 11-month progression-free survival (PFS) and 20-month overall survival (OS). The first-line treatment of sintilimab plus gemcitabine combined with HIFU demonstrates a potential therapeutic effect on metastatic pancreatic carcinoma with tolerable adverse reactions.
Effects of cold water immersion after exercise on fatigue recovery and exercise performance--meta analysis
Authors: Xiao Feiyan , Kabachkova Anastasiia V. , Jiao Lu , Zhao Huan , Kapilevich Leonid V. 
Published: January 2023

Cold water immersion (CWI) is very popular as a method reducing post-exercise muscle stiffness, eliminating fatigue, decreasing exercise-induced muscle damage (EIMD), and recovering sports performance. However, there are conflicting opinions as to whether CWI functions positively or negatively. The mechanisms of CWI are still not clear. In this systematic review, we used meta-analysis aims to examine the effect of CWI on fatigue recovery after high-intensity exercise and exercise performance. A total of 20 studies were retrieved and included from PubMed, PEDro and Elsevier databases in this review. Publication years of articles ranged from 2002 to 2022. In selected studies including randomized controlled trials (RCTs) and Crossover design (COD). Analyses of subjective indicators such as delayed-onset muscle soreness (DOMS) and ratings of perceived exertion (RPE), and objective indicators such as countermovement jump (CMJ) and blood plasma markers including creatine kinase(CK), lactate/lactate dehydrogenase(LDH), C-reactive protein(CRP), and IL-6 were performed. Pooled data showed as follows: CWI resulted in a significant decline in subjective characteristics (delayed-onset muscle soreness and perceived exertion at 0 h); CWI reduced countermovement jump(CMJ) significantly at 0 h, creatine kinase(CK) was lowered at 24 h, and lactate at 24 and 48 h. There was no evidence that CWI affects C-reactive protein(CRP) and IL-6 during a 48-h recovery period. Subgroup analysis revealed that different CWI sites and water temperatures have no effect on post-exercise fatigue recovery. Recommended athletes immersed in cold water immediately after exercise, which can effectively reduce muscle soreness and accelerate fatigue recovery.
AMMI and GGE biplot analyses of Bambara groundnut [Vigna subterranea (L.) Verdc.] for agronomic performances under three environmental conditions
Authors: Esan Vincent Ishola , Oke Grace Oluwasikemi , Ogunbode Timothy Oyebamiji , Obisesan Idowu Arinola 
Published: January 2023

IntroductionThe two most common styles to analyze genotype-by-environment interaction (GEI) and estimate genotypes are additive main effects and multiplicative interaction (AMMI) and genotype + genotype × environment (GGE) biplot. Therefore, the aim of this study was to find the winning genotype(s) under three locations, as well as to investigate the nature and extent of GEI effects on Bambara groundnut production.MethodsThe experiment was carried out in the fields of three environments with 15 Bambara groundnut accessions using the randomized complete block design (RCBD) with three replications each in Ibadan, Osun, and Odeda. Yield per plant, fresh seed weight, total number of pods per plant, hundred seed weight, length of seeds, and width of seeds were estimatedResultsAccording to the combined analysis of variance over environments, genotypes and GEI both had a significant (p &lt; 0.001) impact on Bambara groundnut (BGN) yield. This result revealed that BGN accessions performed differently in the three locations. A two-dimensional GGE biplot was generated using the first two principal component analyses for the pattern of the interaction components with the genotype and GEI. The first two principal component analyses (PCAs) for yield per plant accounted for 59.9% in PCA1 and 40.1% in PCA2. The genotypes that performed best in each environment based on the “which-won-where” polygon were G8, G3, G2, G11, G6, and G4. They were also the vertex genotypes for each environment. Based on the ranking of genotypes, the ideal genotypes were G2 and G6 for YPP, G1 and G5 for FPW, G15 and G13 for TNPP, G3 and GG7 for HSW, G7 and G12 for LOS, and G10 and G7 for WOS. G8 was recorded as the top most-yielding genotype. G8, G4, G7, and G13 were high yielding and the most stable across the environments; G11, G14, and G9 were unstable, but they yielded above-average performance; G14, G12, G15, and G1 were unstable and yielded poorly, as their performances were below average. Bowen was the most discriminating and representative environment and is classified as the superior environment.DiscussionBased on the performance of accessions in each region, we recommend TVSU 455 (G8) and TVSU 458 (G3) in Bowen, TVSU 455 (G8) and TVSU 939 (G6) and TVSU 454 (G1) in Ibadan, and TVSU 158 (G2) and TVSU 2096 (G10) in Odeda. The variety that performed best in the three environments was TVSU 455 (G8). They could also be used as parental lines in breeding programs.
Measuring optimal psychological states: Proposal of two brief versions to measure flow and clutch in athletes
Authors: Sánchez Vara Alejandro , Chamorro José L. , Moreno Castellanos Ruben , Alcaraz Saul 
Published: January 2023

BackgroundThe study of sports experiences with high levels of performance has led to the observation of two psychological states categorized as optimal, denominated flow and clutch. The objective of this study is to design and validate two brief scales version to measure flow and clutch.MethodsFollowing the MIMO (Maximum Information, Minimum Discomfort) protocol, three studies are carried out: In Study 1, we developed the short versions of the questionnaires based on theory-driven and data-driven criteria; in Study 2, we used quantitative criteria to validate the short versions; and in Study 3, we checked the final version of each scale to observe their statistical validity and their relation to other variables.ResultsThe new short flow scale is made up of seven items, while the clutch scale is made up of five items. Throughout the three studies, evidence is provided of the internal consistency, invariance of the measurement models and content validity, validity related to the responding process and validity in relation to other variables.ConclusionThis study provides two short versions to evaluate flow and clutch, which will help to continue advancing in the study of optimal psychological states in sport.
Risk factors for violent behaviors in patients with schizophrenia: 2-year follow-up study in primary mental health care in China
Authors: Huang Zhuo-Hui , Wang Fei , Chen Zi-Lang , Xiao Yao-Nan , Wang Qian-Wen , Wang Shi-Bin , He Xiao-Yan , Migliorini Christine , Harvey Carol , Hou Cai-Lan 
Published: January 2023

ObjectiveThe consequences and impact of violent behavior in schizophrenia are often serious, and identification of risk factors is of great importance to achieve early identification and effective management.MethodsThis follow-up study sampled adult patients with schizophrenia in primary mental health care in a rural area of southern China, in which 491 participants completed a comprehensive questionnaire at baseline and the 2-year follow-up. Sociodemographic, clinical and psychological assessment data were collected from all participants. Paired sample T-Tests and the McNemar Test were performed to examine changes over the follow-up period. Generalized Estimating Equations (GEE) were used to analyze the risk factors for violent behavior.ResultsThe results showed that about two in five community-dwelling patients with schizophrenia reported violent behavior in the past year. At follow-up, participants were significantly less employed, had more times of hospitalization, more psychotropic medication, and severer depressive symptoms, but had better health-related quality of life than at baseline. Use of clozapine and better insight into medication decreased the possibility of violent behavior, while more severe positive symptoms, insomnia, as well as use of second-generation antipsychotics other than clozapine, antidepressants and mood stabilizers increased the possibility of violent behavior.ConclusionsRisk evaluation, prevention and management of violence in patients with schizophrenia are demanded in primary mental health care.
“Crisis” or “opportunity”? COVID-19 pandemic's impact on environmentally sound invention efficiency in China
Authors: Wei Xuan , Liu Ranran , Lin Zhouzhou 
Published: January 2023

IntroductionThe environmentally sound invention (ESI) is a “bridge” between environmental sound technologies (ESTs) and green productions. This study investigates the COVID-19 pandemic's impact on ESI efficiency using a multi-methods model in three stages.MethodsThe ESI efficiency is measured using the Slack-Based Measure (SBM) method in the first stage. By excluding the environmental effect of the pandemic on each province using the stochastic frontier analysis (SFA) model's results in the second stage, this study compares the ESI efficiency change with or without the influence of the pandemic in the third stage.ResultsThe results show that the pandemic can be a “crisis” in the short term, but an “opportunity” in the long term. First, the SBM efficiency results in the first stage show a decrease in the number of the average efficient provinces in which the pandemic is more severe during 2020-2021. Second, results of the spatial Tobit and SFA models provide evidence that the COVID-19 pandemic negatively impacts the ESI efficiency during 2020, this impact is decreasing in 2021, and this impact has a spatial diffusion effect.DiscussionBased on these results, this study discussed the theoretical and political implications. This paper enriches the knowledge of ESTs research and development by proposing a three-stage approach with multi-methods to investigate the influence of the pandemic's impact on ESI efficiency.