bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progress in the Study of TAp73 and Sperm Apoptosis
Authors: Liu Ziao , Pan Min , Li Jingya , Li Li , Wang Tongsheng 
Published: January 2025

ABSTRACTThe study of the mechanism of oligoasthenospermia, which is a major cause of male infertility, has been the focus of research in the field of male reproduction. TAp73, a member of the p53 family of oncogenes, is endowed with tumor‐suppressing activity due to its structural and functional homology with p53. It has been found that TAp73, plays a key role in spermatogenesis and maintaining male reproduction. When TAp73 is low‐expressed or absent, the process of spermatogenesis is severely impaired, and mice deficient in TAp73 exhibit spermatogonial DNA damage, disturbed apical cytoplasmic specialization, and spermatocyte malformations resulting in reduced male fertility. Nevertheless, when TAp73 is overexpressed, it not only drives exogenous death receptors to regulate germ cell apoptosis, but also interacts with its various substrate proteins to promote the translocation of cytoplasmic Bax proteins to the mitochondria, resulting in the upregulation of the Bax/Bcl‐2 ratio on the mitochondrial membrane and triggering a series of mitochondrial apoptotic effects. In this article, we will analyze the mechanism of TAp73 and sperm apoptosis, and elaborate the mechanism of TAp73 upregulation, exogenous apoptosis pathway and mitochondrial apoptosis pathway to systematically explain that the process of apoptosis induced by high expression of TAp73 is not fixed and single, but is interconnected, so as to provide a basis for the treatment of oligoasthenospermia and the research and development of new drugs using TAp73 as a target.
Construction of a Vibrational Circular Dichroism Instrument Equipped With a Thermoelectrically Cooled Detector for Prolonged Measurements
Authors: Sato Hisako , Shimizu Masaru , Watanabe Keisuke , Koshoubu Jun 
Published: January 2025

ABSTRACTA vibrational circular dichroism (VCD) instrument having a thermoelectrically cooled detector (denoted as a TEC unit) was constructed in this study. An electronic device, instead of liquid nitrogen, was employed in the instrument to cool the detector. The feasibility of the system was examined by recording the VCD spectra of liquid pinenes and insect wings. VCD signals were obtained at a constant baseline that was stable for more than 40 h. The spectrometer equipped with a TEC unit exhibited potential for performing measurements for prolonged time periods.
Measuring the impact of a catchment area surveillance tool on cancer center adopters
Authors: Burus Todd , McAfee Caree R. , Wilhite Natalie P. , Hull Pamela C. 
Published: January 2025

AbstractBackgroundThe University of Kentucky Markey Cancer Center developed the data gathering and visualization platform Cancer InFocus (CIF) as a solution for cancer center catchment area surveillance. CIF was released in June 2022 and made available for use to other institutions through a no‐cost licensing agreement. The purpose of this study was to evaluate the impact CIF has had on cancer centers since its release.MethodsThe authors adapted an existing management evaluation framework to assess the impact of CIF across three spheres—idea dissemination, product engagement, and adopter satisfaction. This assessment included an online survey administered between the dates of September 18, 2023 and June 22, 2024 among 28 individuals at 13 CIF adopting institutions.ResultsAs of October 2024, the ideas and approaches of CIF had been disseminated with national audiences 13 times and featured in one peer‐reviewed publication. Thirty‐five institutions, including 26 National Cancer Institute–Designated Cancer Centers, had engaged in licensing CIF. In a user satisfaction survey among adopting institutions, a majority of individuals indicated they were gathering more data (91.7%) and requiring less effort to disseminate data (72.0%) using CIF than under their previous methods.ConclusionsCIF has demonstrated a broad and positive impact on cancer center catchment area surveillance in the 2 years since its release. CIF represents a high value, low‐cost option for cancer centers wanting to build a cancer surveillance dashboard. The framework used for evaluating CIF's impact can be adapted to assess the impact of other open‐source software built and distributed by cancer centers.
Radiation‐Tolerant SRAM for Satellite Image Compression Systems: A Hybrid Memory Array Approach
Authors: Sharma Priyanka , Neema Vaibhav , Singh Chouhan Shailesh , Kumar Soni Nitesh 
Published: January 2025

ABSTRACTThis work is based on the design of an SRAM memory array for on board satellite image compression systems, including memory size, cost, power efficiency, and vulnerability to single event upsets (SEU). Initially, we designed a memory cell, RH_14T, specifically to mitigate the impact of SEUs. Subsequently, we incorporated RH_14T into the on‐board memory array in two variations: RH_14T with minimal area overhead (RH_14T_small) and RH_14T with substantial area overhead (RH_14T_large), which have critical charges of 30.10 and 38.77 fC, respectively. Given the higher sensitivity of higher order bits compared to lower order bits in image pixels, RH_14T_small was allocated for the least significant bit (LSB) positions. RH_14_large, on the other hand, was used for the most significant bit (MSB) positions within the memory array. This configuration enhanced the array's overall area, power, and space radiation tolerance. Furthermore, the RH_14T model was bench marked against other recently introduced radiation‐hardened SRAM cells and compared proposed RH_14T CC18T, RHC14T, RHMC12T, SARP12T, SRRD12T, DICE, and QUCCE12T across several critical design parameters. Notably, RH_14T's sensitive nodes can recover their original data even after radiation‐induced value flips. In addition to these benefits, RH_14T also demonstrates a high static voltage noise margin and reduced read and write delays compared to most of the cells it was compared with.
Design of a Broadband High‐Efficiency Power Amplifier Based on a Miniaturized Three‐Coupled Line Structure
Authors: Zhang Luyu , Zhang Zhiwei , Zhou Dengfa , Wang Chenlu , Gu Chao 
Published: January 2025

ABSTRACTThe extended continuous inverse Class‐GF mode with a large available impedance space is employed in the design of the multioctave power amplifier (PA) presented in this paper. A novel miniaturized three‐coupled line structure is combined with the extended continuous inverse Class‐GF mode to achieve ultra‐wideband high‐efficiency PAs. The novel miniaturized three‐coupled line structure is analyzed in detail to meet the design requirements. To verify the effectiveness of the proposed method, a PA working in 0.7–3.0 GHz with a relative bandwidth of 124.3% is designed and fabricated by using CGH40010F. Measurements demonstrate that the output power is from 39.8 to 42.3 dBm, the drain efficiency is between 63.2% and 73.1%, the power added efficiency is from 60.4% to 71.3%, and the gain is from 9.7 to 12.3 dB. The designed PA achieves wide bandwidth and high efficiency while effectively reducing size to facilitate integration.
Outage Performance of User‐Assisted Energy Harvesting Full Duplex MIMO‐NOMA Networks
Authors: Demirkol Büşra , Kucur Oğuz 
Published: January 2025

ABSTRACTThis work focuses on analyzing the outage probability (OP) in a downlink multi‐user multiple‐input multiple‐output non‐orthogonal multiple access (MIMO‐NOMA) system, specifically employing the maximum‐ratio transmission/receive antenna selection structure. In the investigated system, the user close to the base station (BS) acts as a full‐duplex (FD) (or half‐duplex [HD]) energy harvesting (EH) relay adopting decode‐and‐forward (DF) protocol for transmission to the far user. With and without a direct link, we derive the exact and asymptotic OP expressions in closed form for Nakagami‐ fading channels. The analytical results are validated through simulations, which show that the presence of a direct link in the system significantly enhances the performance of the far user. This improvement occurs because the direct link mitigates the performance degradation caused by the self‐interference of the near‐user. Additionally, the far user exhibits superior performance when the near user operates in the FD mode to the HD mode. Moreover, as the number of transmit antennas of the BS increases, the signal‐to‐noise ratio gains are more pronounced in FD mode than in HD mode. For both HD and FD modes, the performance is better when the number of antennas at the BS is higher than that of the users.
Current Strategies and Future Dimensions in the Development of KRAS Inhibitors for Targeted Anticancer Therapy
Authors: D'Alessio‐Sands Laura , Gaynier Joshua , Michel‐Milian Victoria , Agbowuro Ayodeji A. , Brackett Christopher M. 
Published: January 2025

ABSTRACTKRAS is a proto‐oncogene that is found to be mutated in 15% of all metastatic cancers with high prevalence in pancreatic, lung, and colorectal cancers. Additionally, patients harboring KRAS mutations respond poorly to standard cancer therapy. As a result, KRAS is seen as an attractive target for targeted anticancer therapy. Over the last decade, this protein has evolved from being termed “undruggable” to producing two clinically approved drugs along with several more in clinical development, and many under preclinical investigations. This review details the development of various KRAS‐targeted molecules with emphasis on the different drug design strategies employed by examining the following areas: (1) Direct inhibition of KRAS mutants using small molecule binders, (2) Inhibiting the activated state of KRAS mutants using a binary complex of small molecule binders and cyclophilin A, and (3) Targeted degradation of KRAS mutants using the PROTAC approach. We assess the pharmacological attributes and possible clinical benefits of the different molecules and look to the next frontiers in the application of KRAS inhibitors as anticancer agents.
Effect of Pre‐Processing Treatment and Concentration of Alaria esculenta, Saccharina latissima, and Laminaria digitata Varieties on Texture and Consumer Attribute Preference of Crackers
Authors: Ledbetter Moira , Ross Katrina , Templeman James , Chu Boon‐Seang , Wilkin Jonathan D. 
Published: January 2025

ABSTRACTThis study investigates the effects of three brown seaweed species (Alaria esculenta, Saccharina latissima, and Laminaria digitata), their pre‐processing treatments, and incorporation percentages on the physical and sensory properties of crackers. Significant (p ≤ 0.001) two‐way and three‐way interactions were observed for moisture content, with seaweed addition generally resulting in drier crackers. Shrinkage was primarily influenced by sample thermal treatment, while hardness was significantly affected by seaweed species, treatment, and their interactions. The freeze–thaw treatment produced harder crackers compared to other treatments. Sensory analysis using temporal dominance of sensations (TDS) revealed variations in dominant attributes across different seaweed species and treatments, with retorted and freeze–thaw treatments enhancing crunchiness and reducing fishy flavors. The study demonstrates the potential of seaweed as a functional ingredient in cracker formulations, affecting both textural properties and sensory experiences. It also highlights the importance of pre‐processing treatments in modulating these effects, providing valuable insights for the development of seaweed‐fortified food products with enhanced nutritional value and consumer acceptability.
Effects of Temporary Rearing on the Muscle Flavor Quality of Chinemys reevesii
Authors: Lu Xiaorong , Yu Yali , Dong Lixue , He Gang , Zhang Lang , Mao Tao , Liu Yun , Zhou Yuntao , He Li 
Published: January 2025

ABSTRACTChinemys reevesii, a special economic aquaculture species in China, is valued highly for its medicinal and nutritional benefits. However, the muscle of farmed C. reevesii exhibits a strong off‐flavor, resulting in poor flavor quality. To enhance the flavor quality of the C. reevesii meat, this study examined the volatile compounds in C. reevesii muscle by establishing identification methods for these volatile odor compounds and comparing the differences between the two aquaculture modes. It also explored the impact of fasting temporary rearing (60 days) on the flavor quality of the C. reevesii meat. The results indicated that a combination of direct solvent extraction‐solvent‐assisted flavor evaporation (DSE‐SAFE), headspace‐solid phase microextraction (HS‐SPME), gas chromatography–mass spectrometry/olfactometry (GC–MS/O), and aroma recombination effectively simulated the odor profile of C. reevesii meat. The study identified the key volatile odor compounds in outdoor pond‐cultivated C. reevesii meat (OP), including hexanal, heptanal, 1‐octen‐3‐ol, octanal, nonanal, (E)‐2‐nonenal, decanal, (E)‐2‐decenal, (E,E)‐2,4‐decadienal, and dodecanal. In greenhouse‐cultivated C. reevesii meat (GH), key volatile odor compounds were hexanal, hexanol, heptanal, 1‐octen‐3‐ol, octanal, nonanal, (E)‐2‐nonenal, decanal, (E)‐2‐decenal, and dodecanal. The study found that appropriate fasting temporary rearing improved the odor quality of C. reevesii meat; OP temporary rearing for 40–50 days showed a significant reduction in greasy and musty odors. In GH, 40 days temporary rearing retained a fresh aroma while reducing the seaweed and fishy odors, achieving a flavor similar to OP. This study provides data support for C. reevesii meat processing and suggests a reference for applying temporary rearing to enhance the flavor quality of aquatic products.
An Insight Into the Phytochemical Composition, Cardioprotective, and Antioxidant Characteristics of Small Knotweed (Polygonum plebeium R. Br.) Extract and Its Derived Fractions
Authors: Ibrar Muhammad , Khan Mir Azam , Khan Abdullah , Khan Muhammad Asghar , Jan Muhammad Saeed , Rauf Abdur , Khalil Anees Ahmed , Khalid Ahood , Shahid Samiah , Quradha Mohammed Mansour 
Published: January 2025

ABSTRACTPolygonum plebeium, a member of the Polygonaceae family, is commonly known as small knotweed and has been traditionally used to treat various ailments, including cough, gastrointestinal disorders, respiratory infections, liver disease, inflammation, dysentery, eczema and ringworms, and other skin conditions. Many studies have suggested that plants belonging to this genus possess strong cardio‐protective potentials. Rats were pre‐treated with crude methanolic extract and other fractions at a dose of 500 mg/kg followed by administration of Isoproterenol hydrochloride after 24 h for 2 days. The cardioprotective effect was determined by investigating the levels of Biomarkers responsible for myocardial infarction (MI). Among all fractions Pp.CF (chloroform fraction) exhibited a significant cardioprotective effect by decreasing the levels of ALT, AST, CPK, and LDH to 74.56 ± 1.45, 95.78 ± 2.75, 156.73 ± 1.84, and 215.55 ± 5.33 IU/L in serum. The same fraction was tested for cardio‐protective potential at a dose of 50, 100, and 250 mg/kg. Pp.CF at a dose of 250 mg/kg exhibited prominent effects and reduced levels of biomarkers responsible for MI. Further investigations confirmed that Pp.CF possesses antihyperlipidemic, membrane stabilizing, and thrombolytic potential which suggests P. plebeium an ideal candidate for natural product isolation which will be helpful in the management of cardiovascular problems.
Simultaneous Application of Electron Beam Irradiation and Freezing as an Effective Method for Shelf Life Extension of Minced Turkey Meat
Authors: Salari Mahdieh , Khatami Moein 
Published: January 2025

ABSTRACTIn this study, minced turkey meat samples were subjected to electron‐beam irradiation with dosages of 0, 1.5, 3, and 5 kGy, and then microbial (mesophilic and psychrotrophic bacteria), physiochemical (pH, water activity [aw], thiobarbituric acid reactive substances [TBARS], and peroxide value [PV]), and sensory (color, odor, texture, and overall acceptability) analyses were performed at 0 and 6 months of freezing storage (−18°C). Results showed that by 5 kGy irradiation and freezing treatments, the counts of psychrotrophic and mesophilic bacteria reduced remarkably (p < 0.05) and reached from 4.44 to 2.05 and from 4.9 to 2.00 log cfu/g, respectively. pH and aw of samples did not change notably after treatment and 6 months of freezing storage, the pH and aw of the minced turkey meat were 5.74 and 0.95 for 5 kGy irradiated samples, respectively. Despite the increase of TBARS and PV values, electron‐beam irradiation at 1.5 and 3 kGy had no notable impact on the sensory characteristics of minced turkey meat. The obtained findings revealed that the simultaneous use of irradiation and freezing is a promising method for the increase of shelf life and preservation of minced turkey meat quality.
Evolving Appetites: Current Evidence and Future Perspectives in Terms of Meat Substitutes in Europe
Authors: Mustapa Muhammad Adzran Che , Baba Yasmina , Baishakhy Smita Dash , Kallas Zein 
Published: January 2025

ABSTRACTConsumers are increasingly aware of the environmental and health impacts of their food choices, leading to changes in consumption behavior. This study examines the consumption patterns and behaviors of European consumers regarding meat substitutes and identifies factors influencing their acceptance as alternative protein sources. The study involved 5000 participants from four European countries—France, Germany, Italy, and Spain with data extracted from the Mintel consumer database in 2024. Results indicate that over 50% of consumers in these countries have reduced their intake of red meat, with a notable portion of German consumers adhering to a vegan diet. Across the sample, females significantly differ from males, as they consume less meat, tend to limit their meat intake, and show a greater interest in reducing meat consumption. Baby Boomers predominantly consume more meat and show less interest in reducing their meat intake compared to younger generations. Economic and sustainability aspects are key perceptions driving the perceived benefits of reducing meat consumption. Additionally, preferences for locally sourced products, meat‐like taste and texture, and natural ingredients are primary considerations when purchasing meat substitutes. Fish substitutes were rated as popular meat substitutes in France, Italy, and Spain, while breaded or battered meat/poultry substitutes were the most popular choice in Germany. Insights from this study are valuable for market researchers and the food industry, offering guidance on supplying appealing and sustainable protein alternatives that encourage a shift toward healthier and more sustainable consumption behavior.
Selection and Validation of Appropriate Reference Genes for qRT‐PCR Analysis of Iris germanica L. Under Various Abiotic Stresses
Authors: Yuan Yuan , Liu Chungui , Bao Jianzhong , Li Fengtong 
Published: January 2025

ABSTRACTChoosing the appropriate reference genes for quantitative real‐time PCR (qRT‐PCR) is very important for accurately evaluating expression of target genes. Iris germanica L. is a widely used horticultural plant with high ornamental value, which also shows a strong ability to tolerate abiotic stresses. No comprehensive research has been carried out on optimal reference genes in Iris germanica L. under abiotic stress. In this study, nine reference genes were selected as candidates based on the transcriptome sequencing data of Iris germanica L. The assessment of expression stability under various abiotic stress was conducted using four distinct methods (GeNorm, NormFinder, BestKeeper, and RefFinder). It was found that the optimal reference genes were ACT and F3H for drought and different temperature stresses. EF1α and ACT exhibited superior performance under salt stress. The expression of the IgP5CS gene was evaluated to provide additional validation for the accuracy of the selected optimal reference genes, indicating that inappropriate may lead to significant deviations in the results. This research identified reliable reference genes in I. germanica L. across various abiotic stress conditions, thereby facilitating the investigation into the molecular mechanisms responsible for stress tolerance in I. germanica L.
Fat vs. Sugar: The Case for a Saturated Fat Tax in Italy
Authors: di Cosmo Valeria , Tiezzi Silvia 
Published: January 2025

ABSTRACTWhen judging the distributional impact of unhealthy food taxes, what matters is not just how much low income people would pay but how much the such taxes would benefit or harm them overall. In this paper, we assess the consumer welfare impact of a fat tax net of its expected benefits computed as savings from weight loss. Using Italian data, we estimate a censored Exact Affine Stone Index (EASI) incomplete demand system for food groups, simulating changes in purchases, calorie intake, consumer welfare, and the monetary value of short‐run health benefits. While the Italian government has proposed a sugar tax, we show that there is no significant excess consumption of added sugars among Italian adults. Instead, excessive fat consumption is more prevalent, making a fat tax a more compelling and effective solution to address diet‐related health risks. Our results suggest costs from fat taxation are larger than benefits at all income levels. As a fraction of income, the net impact would be slightly regressively distributed.
Impacts of Housing Costs on Health and Satisfaction With Life Circumstances: Evidence From Australia
Authors: Abayasekara Ashani , Kim Jun Sung , Wang Liang Choon 
Published: January 2025

ABSTRACTThis paper examines the causal impacts of rising housing costs on individual health and satisfaction with life circumstances, using a fixed‐effects instrumental variable approach and individual‐level panel data from Australia. Relying on the historical patterns of immigrant settlement, we construct an instrumental variable that exploits exogenous variation in housing costs driven by foreign investments that flow differentially into localities. We find that rising housing costs—as measured by composite housing costs faced by homeowners and renters living in an area—have a significant positive impact on individuals' self‐assessed physical health and a significant negative impact on satisfaction ratings, but no significant impact on self‐assessed emotional health. Heterogeneity analysis suggests that the positive effects on physical health are mainly concentrated among homeowners, the well‐educated, and older individuals.