bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of an active focused intervention on the quality of life among elderly individuals in the slum areas of Thane district in India: An experimental field-based randomized clinical trial
Authors: Parab Bhadrapriya , Parab Sanjay , Velhal Gajanan 
Published: July 2023

Care-giving to elderly population is challenging impacting their quality of life (QoL). We carried out the present study evaluating the influence of an intervention targeting this population in improving their QoL. A community-based randomized clinical trial was undertaken targeting the elderly population residing in an urban slum area after evaluating the baseline QoL using pre-validated questionnaire from World Health Organization (WHO QOL-BREF). Intervention involving mainly the interaction with elderly population in facilitating their interaction with the health care providers, and social clubs was carried out in the intervention group. Control group of participants was provided the usual standard of care without any active intervention.: Eight-hundred and fifty-two were included (study group: 426 and control group: 426) with a large-majority in the age range of 60-69 years. Most of the participants were educated, housewives, economically dependent on their family members, with a monthly income ranging between 5001 and 10000 rupees. The demographic characteristics were comparable between the intervention and control groups except for the concomitant diseases that were significantly more in the intervention group. Baseline mean WHO QOL-BREF scores were 40.04 and 45.2 in the intervention and control groups, respectively. Post-intervention, the QoL scores were significantly (p &#60; 0.05) greater in the intervention group. Those between 60 and 69 years, male gender, those independant on their family members, and healthy individuals had significantly greater QoL scores post-intervention.We observed that our intervention had significant beneficial effects in improving the QoL among the elderly individuals residing in an urban slum area. We have also identified certain sub-groups of individuals with a greater response. There is an urgent need for evaluating the utility of our intervention using a randomized study.
Evaluation of association between the quality of life of urban senior citizens living in the slum area and their socio-demographic factors: A community-oriented approach
Authors: Parab Bhadrapriya Sanjay , Parab Sanjay , Velhal Gajanan 
Published: July 2023

Factors influencing the quality of life (QoL) of senior citizens are many. We evaluated the factors affecting various domains of quality of life in this vulnerable population. A community-based survey was conducted in the Thane District of Maharashtra state, India amongst 427 individuals above 60 years of age. A pre-validated World Health Organization quality of life questionnaire (WHOQOL-BREF) followed by personal interviews was used.Overall a lower QoL was observed amongst the study participants. Advanced age, female gender, informal education, economically dependence on the family members, low socio -economic status and those with multiple health problems were observed to be associated with poor QoL. We have identified factors significantly associated with poor QoL amongst the senior citizens living in a slum area. Interventions targeting this vulnerable population (particularly with risk factors) should be explored for their utility in improving the QoL.
CHILD FEEDING PRACTICE, SANITATION HYGIENE, AND OTHER FACTORS OF DIARRHEA IN CHILDREN AGED 6-23 MONTHS IN INDONESIA: ANALYSIS OF IDHS 2017
Authors: Sani Salma Luthfiyah , Achadi Endang Laksminingsih 
Published: July 2023

Diarrhea remains one of the major contributors of under five children mortality worldwide. IDHS 2017 data show that 6-23 month-old children have the highest diarrhea prevalence. The objective of this research is to analyze the association of child feeding practices (early initiation of breastfeeding, continued breastfeeding, bottle feeding, MDD, MMF, MAD); sanitation hygiene (source of drinking water, treatment of drinking water, toilet facility, handwashing facility); healthcare utilization factors (immunization status, vitamin A in the last 6 months); and socio-demographic factors (maternal age, maternal education, maternal working status, economic status, residence) with diarrhea incidence in 6-23 month-old children in Indonesia. This cross-sectional study included a total of 4,030 children aged 6-23 months from IDHS 2017. Chi-square test and multiple logistic regression were applied to analyze factors associated with diarrhea. It was found that the prevalence of diarrhea was 19.8% in 2017. Bivariate analysis shows that diarrhea was significantly associated with continued breastfeeding, bottle feeding, toilet facility, source of drinking water, maternal age, economic status, and place of residence. Furthermore, multivariate analysis results in some variables have a statistically significant association with diarrhea, which are the use of bottle feeding, non-improved toilet facility, maternal age 15-29 years, and low maternal education. The dominant factor associated with diarrhea was toilet facility (OR=1.500, 95% CI 1.262-1.784). Healthy latrines utilization, cleaning baby bottle properly, and enhancing health education for mothers are pivotal to prevent diarrhea and maintain children’s health at 6-23 months.
Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress
Authors: Vorvul A. O. , Bobyntsev I. I. , Mishina E. S. , Medvedeva O. A. , Andreeva L. A. , Myasoedov N. F. 
Published: July 2023

Aim. To study the morphofunctional state of the colonic wall in rats when using the N–terminal analog of the adrenocorticotropic hormone (ACTH) ACTH6-9-Pro-Gly-Pro (ACTH6-9-PGP) peptide under chronic stress.Materials and methods. The study was performed on 55 male Wistar rats, which were divided into 5 groups (n = 11): group 1 – control group (administration of saline solution without stress); group 2 – chronic restraint stress (CRS) + administration of saline solution; group 3 – CRS + administration of ACTH6-9-PGP at a dose of 5 μg / kg; group 4 – administration of ACTH6-9-PGP at a dose of 50 μg / kg; group 5 – administration of ACTH6-9- PGP at a dose of 500 μg / kg. A histologic examination of the rat colon was performed. The histologic architecture of the colonic wall, the depth of crypts, and the number of goblet cells were assessed. Furthermore, the number of granulocytes, plasma cells, lymphocytes, macrophages, and mast cells was counted.Results. The study demonstrated that chronic (14 days) restraint stress resulted in the development of inflammations in the colonic wall of the animals. Intraperitoneal administration of ACTH6-9-PGP at doses of 50 and 500 μg / kg daily throughout the entire time of stress exposure prevented the development of stress-induced alterations observed in the control animals. At the same time, anti-inflammatory effects of the peptide in the colonic wall and a decrease in the level of corticosterone in the blood serum were noted.Conclusion. The results of this work and data from other studies on the effects of N-terminal analogs of ACTH indicate the need for studying the mechanisms of their effect on inflammation and searching for targets of ACTH6-9-PGP.
Modern concepts in cervical carcinogenesis
Authors: Naumova L. A. , Starodumova V. A. 
Published: July 2023

The article discusses modern ideas about cervical carcinogenesis as a multi-stage process of multifactorial genesis. Currently, ideas about the pathogenesis of cervical cancer (CC) are based not only on understanding the role of high-risk oncogenic human papillomavirus (HPV) in this process and accumulation of genetic changes caused by it, but also on formation of a complex HPV interactome, or a network of intermolecular interactions of HPV oncoproteins with host cell proteins. Carcinogenesis also involves a wide range of epigenetic events and, above all, impairment of the regulatory function of miRNAs. An important role in cervical carcinogenesis is attributed to the concept of cancer stem cells (CSCs) formulated in recent years, which is closely related to the explanation of disease recurrence and treatment resistance, as well as to new approaches to treatment. The cervicovaginal microbiome and cervical microenvironment, which are responsible for natural clearance of HPV, regression of epithelial lesions, and modeling of the immune response, are becoming promising objects for research.The aim of the review was to present up-to-date information on the most important mechanisms of cervical carcinogenesis, as well as on new approaches to the treatment of CC, based, in particular, on the use of knowledge about regulatory miRNAs, CSC markers, and the state of the cervicovaginal microbiota.
Study of molecular genetic markers of Gilbert’s syndrome
Authors: Ivanova A. A. , Gurazheva A. A. , Mel’nikova E. S. , Maksimov V. N. , Nemcova E. G. 
Published: July 2023

Aim. To study new molecular genetic markers of Gilbert’s syndrome (GS).Materials and methods. It was a case – control study. The GS group included 125 people (mean age 38.5 ± 11.9 years, 58.9% were men) with unconjugated hyperbilirubinemia; known causes of unconjugated hyperbilirubinemia were excluded. The control group (n = 323, mean age 48.9 ± 11.9 years, 53.2% were men) was a random sample of individuals from the DNA bank of participants of the HAPIEE and MONICA projects. DNA was isolated by phenol – chloroform extraction from venous blood. Genotyping of groups by rs3064744, rs34993780, rs56059937, rs4148323, and rs4124874 single nucleotide polymorphisms (SNPs) in the UGT1A1 gene was performed by polymerase chain reaction followed by the polyacrylamide gel analysis according to the author’s protocols.Results. For rs34993780 and rs56059937, no carriers of a rare allele were found in the GS group and the control group. In the GS group, two carriers of a heterozygous mutation rs4148323 were found. Statistically significant differences between the groups were found in the frequencies of rs4124874: homozygous GG was statistically significantly more common in the GS group than in the control group (odds ratio (OR) = 11.8, 95% confidence interval (CI) 6.9–20.3, p < 0.001).Conclusion. The GG genotype of rs4124874 in the UGT1A1 gene is associated with an increased risk of GS. Carriers of the rare heterozygous mutation rs4148323 were found in the GS group.
Galectin-3 in the blood serum of patients with bone tumors
Authors: Kushlinskii N. E. , Kovaleva O. V. , Prishchep P. L. , Zybina N. N. , Jurisic V. , Alferov A. A. , Kuzmin Yu. B. , Goryacheva I. O. , Kuznetsov I. N. , Bulytcheva I. V. , Varfolomeeva S. R. , Sushentsov E. A. , Gershtein E. S. , Rogozhin D. V. , Yanushevich O. O. , Stilidi I. S. 
Published: July 2023

Background. Due to diversity of cancer, the functional role of galectin-3 is rather controversial; however, for many types of neoplasms, the marker acts as a tumor growth promoter.Aim. To perform a comparative analysis of galectin-3 levels in the blood serum of healthy individuals and patients with benign, borderline, and malignant bone tumors divided into two age groups (under and over 18 years of age) based on the main clinical and morphological characteristics of the disease and prognosis.Materials and methods. The study included 201 patients with benign, borderline (giant cell tumors, locally aggressive tumors), and malignant bone tumors and 31 healthy donors. The galectin-3 level was determined in the blood serum before treatment with Human Galectin-3 ELISA kit (R&D, USA).Results. The level of galectin-3 in the blood serum of patients with benign and malignant bone tumors was statistically significantly higher than that in the control group of patients both under and over 18 years. In patients with borderline bone tumors, a trend toward an increase in the galectin-3 concentration compared with the controls was revealed. The ROC analysis for galectin-3 in patients with bone sarcomas showed that the area under the curve (AUC) comprised 0.795 (р < 0.0001) in the group of patients over 18 years and 0.868 (р = 0.0008) in the individuals under 18 years. For malignant bone tumors in patients over 18 years, the sensitivity of this method was 71.3%, and specificity was 71.43% (optimal cut-off level was 8.09 ng / ml; р < 0.0001), while in patients under 18 years, the sensitivity of the method was 80%, and specificity was 90% (optimal cut-off level was 5.49 ng / ml; р < 0.001). No significant associations between the serum galectin-3 level and the clinical and morphological characteristics of bone neoplasms were found both in patients under and over 18 years of age. However, it could be noted that the highest concentration of the marker was found in chordomas and at earlier stages of the disease. In patients over 18 years with chondrosarcoma and osteosarcoma, no correlation between the marker and the disease prognosis was found.Conclusion. An increase in the galectin-3 level in the blood serum was observed in all age groups of patients with both benign and malignant bone tumors. However, the sensitivity and specificity of the method assessed by the ROC analysis do not allow to apply this marker for the diagnosis of bone tumors.
T cells targeting peripheral nerves are expanded and shared in patients with Guillain-Barré Syndrome.
Authors: Súkeníková* Lenka , Mallone* Anna , Schreiner Bettina , Ripellino Paolo , Nilsson Jakob , Sallusto Federica , Latorre Daniela 
Published: July 2023

Abstract
Guillain-Barré syndrome (GBS) is a rare and potentially life-threatening disorder of the peripheral nervous system usually triggered by a preceding infection and causing a rapidly progressive muscle weakness. While the disease is generally considered to have an autoimmune basis, the cellular and molecular players underlying its immunopathology are mostly elusive. By combining in vitro T cell screenings, generation of T cell clones and high throughput TCRVβ sequencing, we reveal the existence of autoreactive memory CD4+ and rare CD8+ T cells targeting myelin antigens of the peripheral nerves in GBS patients. The characterization of more than 1000 autoreactive single T cell clones uncovered a polyclonal TCR repertoire and short CDR3β lengths, a preferential HLA-DR restriction and immunodominant epitopes recognition across patients. Autoreactive clonotypes were expanded in the blood of the same patient at different disease stages and, importantly, were shared in the blood and cerebrospinal fluid of different GBS patients, but not in control individuals. Finally, myelin-reactive CD4+ T cells were identified in the nerve biopsy of one GBS patient, indicating their direct contribution to disease physiopathology. Collectively, our data provide the first description of autoreactive T cells in GBS patients and open new perspectives in the field of peripheral inflammatory neuropathies, with potential significant translational impact for biomedical applications.
Investigation of DC and RF Characteristics of Recessed Gate III-Nitride nano-HEMT on β-Ga2O3 Substrate with Back-Barrier for High-Power and High-Frequency Applications
Authors: Rao G. Purnachandra , Lenka Trupti Ranjan , Boukortt Nour El. I. , Nguyen Hieu Pham Trung 
Published: July 2023

Abstract
In this research study, a recessed gate III-Nitride based nano high electron mobility transistor (HEMT) grown on a lattice matched β-Ga2O3 substrate is designed. This research investigation aims to enhance DC and RF performance of AlGaN/GaN HEMT, and minimize the short-channel effects by incorporating an AlGaN back layer and field plate technique, which can enhances electron confinement in two-dimensional electron gas (2DEG). A precise comparison analysis is done on the proposed HEMT’s input characteristics, output characteristics, leakage current characteristics, breakdown voltage properties, and RF behaviour in presence and absence of AlGaN back layer in regards to field plate configuration. The inclusion of back barrier aids in raising the level of conduction band, which reduces leakage loss beneath the buffer, and aids in keeping the 2DEG to be confined to narrow channel. Furthermore, the field plate design offers an essential electric field drift between gate and drain, resulting to enhanced breakdown voltage characteristics. The proposed recessed gate HEMT with intended Al0.1Ga0.9N layer between GaN buffer and β-Ga2O3 substrate along with field-pated arrangement exhibited a superior performance attributes.
Does the Low Carbon Transition Impact Urban Resilience？ Evidence from China's Pilot Cities for Carbon Emissions Trading
Authors: zhang sisi , Ma Xiaoyu , cui qi , liu jiamin 
Published: July 2023

Abstract
The low-carbon transition is a systemic economic and social change that will inevitably have an impact on many areas of the urban system. Among them, has China's ongoing low-carbon transition had an impact on urban resilience (UR) systems while achieving urban energy saving and carbon emission reduction goals? This paper uses the implementation of the carbon emissions trading pilot policy (CETPP) as a "quasi-natural experiment", and evaluates the impact of the policy on UR using a difference-in-differences model based on the data of prefecture-level cities from 2011 to 2020. The study shows that pilot carbon trading policies have a favorable impact on UR, and the market mechanism of carbon emissions has a heterogeneous cause influence on UR. The impact of pilot carbon trading policies on UR varies according to the respective moderating effects of institutional factors, green technology innovation, industrial structure rationalization, and output effects.
Towards sustainable transport: quantifying and mitigating pollutant emissions from heavy-duty diesel trucks in Northeast China
Authors: Li Na , Gao Chengkang , Ba Qiao , You Huan , Zhang Xinhong 
Published: July 2023

Abstract

Heavy-duty diesel trucks (HDDTs) have caused serious environmental pollution in China. Accurate estimation of their pollutant emission characteristics is essential to reduce emissions and associated environmental and public health impacts. We developed localized emission factors and a high-resolution emission inventory of HDDTs in Northeast China, based on on-board test, Guidebook and international vehicle emission (IVE) model. The results show that the total emissions of CO, NO, NO2 and PM from HDDTs in Northeast China in 2020 were 172.2 kt, 531.5 kt, 11.2 kt and 921.4 t, respectively. In terms of spatial distribution, emissions decreased from the city center to the city fringe. Temporally, the NOx emission variation curves of different types of roads presented a "single-peak" emission characteristic, which was different from the peak of traffic flow. We further developed three emission reduction scenarios to analyze the pollution reduction under different policies. Scenario analysis shows that elimination of HDDTs that follow the old China III emission standard and installing tailpipe treatment devices are the most effective pollutant reduction measure. The reduction percentages for CO, NO, NO2 and PM ranged from 62.9–83.89%. The results of our study could inform policymakers to devise feasible strategies to reduce vehicle pollution in Northeast China.
LRP1B affects lung adenocarcinoma immunotherapy efficacy via ferroptosis
Authors: ke zihao , yu tao , zhang qi , chen ying , lu kaihua 
Published: July 2023

AbstractBackground Currently, the clinically used immune biomarkers for non-small cell lung cancer (NSCLC) are programmed death ligand 1 (PD-L1) and tumor mutation burden (TMB). However, they cannot accurately predict the effectiveness of immunotherapy. Identifying appropriate biomarkers that can differentiate between beneficiary groups is imperative. Methods We identified LRP1B as a potential biomarker for immunotherapy by analyzing clinical data, combined with bioinformatics analysis. The effects of LRP1B on ferroptosis were assessed using qRT-PCR, Western blotting, CCK-8 assay, and flow cytometry. The potential mechanism underlying the regulation of ferroptosis by LRP1B was elucidated using qRT-PCR, Western blotting, ChIP, and dual-luciferase reporter gene assays. Results Through the collection and analysis of clinical data, we had established that LRP1B mutation is a highly promising biomarker for immunotherapy. Bioinformatics analysis revealed significant differences in the expression levels of PD-L1 and TMB between patients with LRP1B mutation and wild-type patients in lung adenocarcinoma (LUAD), while in lung squamous cell carcinoma (LUSC), only the level of TMB showed a significant difference. Furthermore, we observed that patients with LRP1B mutation in LUAD had significantly higher levels of tumor-infiltrating lymphocytes (TILs), including CD4+T cells and M1 macrophages, than wild-type patients, whereas in LUSC, no such differences were detected. In addition, we found that patients with LRP1B mutation in LUAD had significantly prolonged progression-free survival (PFS) compared to wild-type patients, but no PFS benefit was observed in patients with LRP1B mutation in LUSC. These findings provided strong evidence that LRP1B mutation was a potential biomarker for immunotherapy in LUAD. Moreover, our in vivo experiments indicated that knockdown of LRP1B enhanced the efficacy of mPD-1, and mechanistic studies revealed that LRP1B regulated the sensitivity of cells to ferroptosis by modulating the expression of SLC7A11 through altering the phosphorylation level of STAT3. Importantly, LRP1B alterations also affected the sensitivity of cells to ferroptosis induced by IFN-γ. Further analysis revealed that LRP1B knockdown promoted immunotherapy in vivo. Conclusions Our results confirmed that LRP1B affected the efficacy of immunotherapy by modulating the sensitivity of NSCLC cells to ferroptosis.
Synergistic interaction of Artemisia cina n-hexanic extract and Tagetes lucida ethyl acetate extract on Haemonchus contortus
Authors: Santiago-Figueroa Itzel , González-Cortázar Manasés , Estrada-Flores Julieta Gertrudis , Cuéllar-Ordaz Jorge Alfredo , López-Arellano Maria Eugenia , González-Reyes Francisco Javier , Olmedo-Juárez Agustín , Piedrahita Rosa Isabel Higuera 
Published: July 2023

Abstract
The present study aimed to analyze the possible synergistic activity among the active extracts from Artemisia cina and Tagetes lucida combinations on the Haemonchus contortus nematode parasite of sheep. The work was carried out in vitro on eggs and infective larvae 3 of H. contortus. Also, the characterization of the extracts was made with HPLC. The results were analyzed with SAS 9.1 apply the anova and Tukey test, and the lethal concentration (LC) LC50 and LC90 were determined with regression analysis employed Proc Probit of SAS 9.1. Additionally, the FT was calculated with LC50 and LC90 to determine the synergistic effect. The results demonstrated high efficacy of the two plants studied on both nematode stages egg and larvae L3, as well as of their combinations in where the best inhibition hatch egg was obtained with 50/50% combination of each plant and the best larvae mortality were obtained with 25% A. cina and 75% T. lucida at 10 mg/ml, additionally this combination showed synergistic effect. In conclusion the two plants are an option as anthelmintic non pharmacological due to high bioactive effect and due to their synergistical response.