bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular cloning, prokaryotic expression and its application potential evaluation of interferon (IFN)-ω of forest musk deer
Authors: Wu Xi , Yang Wei , Cheng Jian-guo , Luo Yan , Fu Wen-long , Zhou Lei , Wu Jie , Wang Yin , Zhong Zhi-jun , Yang Ze-xiao , Yao Xue-ping , Ren Mei-shen , Li Yi-meng , Liu Jie , Ding Hui , Chen Jia-nan 
Published: June 2023

AbstractForest musk deer (Moschus berezovskii) are currently a threatened species under conservation, and the development of captive populations is restricted by health problems. To evaluate the application potential of interferon (IFN)-ω in the prevention and control of forest musk deer disease, 5 forest musk deer IFN-ω (fmdIFNω) gene sequences were successfully obtained by homologous cloning method for the first time. FmdIFNω5 was selected and recombinant fmdIFNω protein (rIFNω) was successfully expressed by pGEX-6P-1 plasmid and E. coli expression system. The obtained protein was used to stimulate forest musk deer lung fibroblasts cells FMD-C1 to determine its regulatory effect on interferon-stimulated genes (ISGs). In addition, an indirect ELISA method based on anti-rIFNω serum was established to detect endogenous IFN-ω levels in 8 forest musk deer. The results showed that there were 18 amino acid differences among the 5 fmdIFNω subtypes, all of which had the basic structure to exert the activity of type I IFN and were close to Cervus elaphus IFN-ω in the phylogenetic tree. The protein expressed was 48 kDa, and the transcription levels of all ISGs were increased in FMD-C1 cells stimulated by rIFNω, and the amount of transcription accumulation was time-dependent. Meanwhile, Anti-rIFNω serum of mice could react with both rIFNω and forest musk deer serum, and the OD450nm value of forest musk deer serum with the most obvious symptoms was the highest, suggesting that the level of natural IFN-ω in different forest musk deer could be monitored by the rIFNω-based ELISA method. These results indicate that fmdIFNω has the potential as an antiviral drug and an early indication of innate immunity, which is of great significance for the prevention and control of forest musk deer diseases.
Wound healing responses of urinary extravasation after urethral injury
Authors: Hyuga Taiju , Fujimoto Kota , Hashimoto Daiki , Tanabe Kazuya , Kubo Taro , Nakamura Shigeru , Ueda Yuko , Fujita-Jimbo Eriko , Muramatsu Kazuhiro , Suzuki Kentaro , Osaka Hitoshi , Asamura Shinichi , Moriya Kimihiko , Nakai Hideo , Yamada Gen 
Published: June 2023

AbstractThe post-surgical fluid leakage from the tubular tissues is a critical symptom after gastrointestinal or urinary tract surgeries. Elucidating the mechanism for such abnormalities is vital in surgical and medical science. The exposure of the fluid such as peritonitis due to urinary or gastrointestinal perforation has been reported to induce severe inflammation to the surrounding tissue. However, there have been no reports for the tissue responses by fluid extravasation and assessment of post-surgical and injury complication processes is therefore vital. The current model mouse study aims to investigate the effect of the urinary extravasation of the urethral injuries. Analyses on the urinary extravasation affecting both urethral mesenchyme and epithelium and the resultant spongio-fibrosis/urethral stricture were performed. The urine was injected from the lumen of urethra exposing the surrounding mesenchyme after the injury. The wound healing responses with urinary extravasation were shown as severe edematous mesenchymal lesions with the narrow urethral lumen. The epithelial cell proliferation was significantly increased in the wide layers. The mesenchymal spongio-fibrosis was induced by urethral injury with subsequent extravasation. The current report thus offers a novel research tool for surgical sciences on the urinary tract.
Micro-architecture design exploration template for AutoML case study on SqueezeSEMAuto
Authors: Chantrapornchai Chantana , Kajkamhaeng Supasit , Romphet Phattharaphon 
Published: June 2023

AbstractConvolutional Neural Network (CNN) models have been commonly used primarily in image recognition tasks in the deep learning area. Finding the right architecture needs a lot of hand-tune experiments which are time-consuming. In this paper, we exploit an AutoML framework that adds to the exploration of the micro-architecture block and the multi-input option. The proposed adaption has been applied to SqueezeNet with SE blocks combined with the residual block combinations. The experiments assume three search strategies: Random, Hyperband, and Bayesian algorithms. Such combinations can lead to solutions with superior accuracy while the model size can be monitored. We demonstrate the application of the approach against benchmarks: CIFAR-10 and Tsinghua Facial Expression datasets. The searches allow the designer to find the architectures with better accuracy than the traditional architectures without hand-tune efforts. For example, CIFAR-10, leads to the SqueezeNet architecture using only 4 fire modules with 59% accuracy. When exploring SE block insertion, the model with good insertion points can lead to an accuracy of 78% while the traditional SqueezeNet can achieve an accuracy of around 50%. For other tasks, such as facial expression recognition, the proposed approach can lead up to an accuracy of 71% with the proper insertion of SE blocks, the appropriate number of fire modules, and adequate input merging, while the traditional model can achieve the accuracy under 20%.
Integration of force and IMU sensors for developing low-cost portable gait measurement system in lower extremities
Authors: Manupibul Udomporn , Tanthuwapathom Ratikanlaya , Jarumethitanont Wimonrat , Kaimuk Panya , Limroongreungrat Weerawat , Charoensuk Warakorn 
Published: June 2023

AbstractGait analysis is the method to accumulate walking data. It is useful in diagnosing diseases, follow-up of symptoms, and rehabilitation post-treatment. Several techniques have been developed to assess human gait. In the laboratory, gait parameters are analyzed by using a camera capture and a force plate. However, there are several limitations, such as high operating costs, the need for a laboratory and a specialist to operate the system, and long preparation time. This paper presents the development of a low-cost portable gait measurement system by using the integration of flexible force sensors and IMU sensors in outdoor applications for early detection of abnormal gait in daily living. The developed device is designed to measure ground reaction force, acceleration, angular velocity, and joint angles of the lower extremities. The commercialized device, including the motion capture system (Motive-OptiTrack) and force platform (MatScan), is used as the reference system to validate the performance of the developed system. The results of the system show that it has high accuracy in measuring gait parameters such as ground reaction force and joint angles in lower limbs. The developed device has a strong correlation coefficient compared with the commercialized system. The percent error of the motion sensor is below 8%, and the force sensor is lower than 3%. The low-cost portable device with a user interface was successfully developed to measure gait parameters for non-laboratory applications to support healthcare applications.
Identification of early stage and metastatic prostate cancer using electrochemical detection of beta-2-microglobulin in urine samples from patients
Authors: Nasimi Hashmatullah , Madsen Jonna Skov , Zedan Ahmed H. , Malmendal Anders , Osther Palle Jörn Sloth , Alatraktchi Fatima AlZahra’a 
Published: June 2023

AbstractTo improve prostate cancer (PCa) diagnosis, it is imperative to identify novel biomarkers and establish effective screening techniques. Here, we introduce electrochemical biosensing of β-2-Microglobulin (β2M) in urine as a potential diagnostic tool for PCa. The immunosensor is composed of a screen-printed graphene electrode coated with anti β2M antibodies. The sensor is capable of detecting the protein directly in urine without any sample pretreatment within 45 min including sample incubation and a lower limit of detection of 204 µg/L. The sensor demonstrated a significant difference in the β2M-creatinine ratio in urine between control and both local- and metastatic PCa (mPCa) (P = 0.0302 and P = 0.0078 respectively), and between local- and mPCa (P = 0.0302). This first example of electrochemical sensing of β2M for the diagnosis of PCa may set the stage for an affordable, on-site screening technique for PCa.
Synthesis of ester-containing phenanthridinesviaphotoredox-catalyzed radical cascade cyclization ofN-arylacrylamides with alkyloxalyl chlorides
Authors: Chen Meiling , Chen Jian-Qiang , Chen Zhengkai , Wu Jie 
Published: June 2023

An alkoxycarbonyl-radical-triggered cascade cyclization reaction of N-(o-cyanobiaryl)acrylamides with alkyloxalyl chlorides is developed, leading to diverse ester-containing phenanthridines.
Does Assessor Masking Affect Kindergartners' Performance on Oral Language Measures? A COVID-19 Era Experiment With Children From Diverse Home Language Backgrounds
Authors: Surrain Sarah , Mesa Michael P. , Assel Mike A. , Zucker Tricia A. 
Published: June 2023

Purpose:
The ongoing COVID-19 pandemic has prompted changes to child assessment procedures in schools such as the use of face masks by assessors. Research with adults suggests that face masks diminish performance on speech processing and comprehension tasks, yet little is known about how assessor masking affects child performance. Therefore, we asked whether assessor masking impacts children's performance on a widely used, individually administered oral language assessment and if impacts vary by child home language background.

Method:

A total of 96 kindergartners (5–7 years old,
n
= 45 with a home language other than English) were administered items from the Clinical Evaluation of Language Fundamentals Preschool–Second Edition Recalling Sentences subtest under two conditions: with and without the assessor wearing a face mask. Regression analysis was used to determine if children scored significantly lower in the masked condition and if the effect of masking depended on home language background.

Results:
Contrary to expectations, we found no evidence that students scored systematically differently in the masked condition. Children with a home language other than English scored lower overall, but masking did not increase the gap in scores by language background.

Conclusions:
Our results suggest that children's performance on oral language measures is not adversely affected by assessor masking and imply that valid measurements of students' language skills may be obtained in masked conditions. While masking might decrease some of the social determinants of communication (e.g., recognition of emotions), masking in this experiment did not appear to detract from children's ability to hear and immediately recall verbal information.

Supplemental Material:

https://doi.org/10.23641/asha.23567463
Generation of synthetic multi‐resolution time series load data
Authors: Pinceti Andrea , Sankar Lalitha , Kosut Oliver 
Published: June 2023

AbstractThe availability of large datasets is crucial for the development of new power system applications and tools; unfortunately, very few are publicly and freely available. The authors designed an end‐to‐end generative framework for the creation of synthetic bus‐level time‐series load data for transmission networks. The model is trained on a real dataset of over 70 Terabytes of synchrophasor measurements spanning multiple years. Leveraging a combination of principal component analysis and conditional generative adversarial network models, the developed scheme allows for the generation of data at varying sampling rates (up to a maximum of 30 samples per second) and ranging in length from seconds to years. The generative models are tested extensively to verify that they correctly capture the diverse characteristics of real loads. Finally, an opensource tool called LoadGAN is developed which gives researchers access to the fully trained generative models via a graphical interface.
Spitzer thermal phase curve of WASP-121 b
Authors: Morello G. , Changeat Q. , Dyrek A. , Lagage P.-O. , Tan J. C. 
Published: June 2023

Aims. We analyse unpublished Spitzer observations of the thermal phase-curve of WASP-121 b, a benchmark ultra-hot Jupiter.
Methods. We adopted the wavelet pixel-independent component analysis technique to remove challenging instrumental systematic effects in these datasets and we fit them simultaneously with parametric light-curve models. We also performed phase-curve retrievals to better understand the horizontal and vertical thermal structure of the planetary atmosphere.
Results. We measured planetary brightness temperatures of ~2700 K (dayside) and ~700–1100 K (nightside), along with modest peak offsets of 5.9° ± 1.6 (3.6 µm) and 5.0°−3.1+3.4 (4.5 µm) after mid-eclipse. These results suggest inefficient heat redistribution in the atmosphere of WASP-121 b. The inferred atmospheric Bond albedo and circulation efficiency align well with observed trends for hot giant exoplanets. Interestingly, the measured peak offsets correspond to a westward hot spot, which has rarely been observed. We also report consistent transit depths at 3.6 and 4.5 µm, along with updated geometric and orbital parameters. Finally, we compared our Spitzer results with previous measurements, including recent JWST observations.
Conclusions. We extracted new information on the thermal properties and dynamics of an exoplanet atmosphere from an especially problematic dataset. This study probes the reliability of exoplanet phase-curve parameters obtained from Spitzer observations when state-of-the-art pipelines are adopted to remove the instrumental systematic effects. It demonstrates that Spitzer phase-curve observations provide a useful baseline for comparison with JWST observations, and shows the increase in parameters precision achieved with the newer telescope.
Les patients dits « non urgents » aux urgences
Authors: Labainville Inès , Lefève Céline 
Published: June 2023

Le phénomène actuel de saturation des services d’urgences est souvent attribué aux passages abusifs des patients relevant de la médecine de ville. Cet article, fondé sur une revue de la littérature médicale et sociologique, interroge cette assertion en examinant l’articulation des définitions médicales et sociales des patients qualifiés de « non urgents » et les manières dont elles se traduisent dans des critères de priorisation, de sélection et d’orientation. Il en ressort que les pratiques de tri, nécessaires pour prioriser les cas d’urgence vitale, ne se fondent pas seulement sur des critères cliniques mais intègrent également des considérations morales et sociales qui peuvent mener à des discriminations et entraver l’accès équitable aux soins, en particulier pour les patients les plus vulnérables.
A New Approach: Determination of the Safe Surgical Margin in Pterygium Surgery
Authors: Dag Yasar , Armutlu Ayse , Acet Yakup 
Published: June 2023

Abstract
Purpose In this retrospective study, we aimed to determine the safe surgical limit for excision of pterygium tissue. Therefore, we aimed to prevent excessive or incomplete normal
conjunctival tissue excision during surgery in the coming years.
Methods Autografted pterygium surgery was performed between January 2015 and April 2016, and the excised pterygium tissue was examined histopathologically. The files of 44 patients,
who had not previously undergone any ocular surgery, who did not have an inflammatory disease and who continued to be checked for at least 1 year, were retrospectively examined. The distance
(P-DSEM) from the excised pterygium tissue to the surgical excision margin was measured by a pathologist. Postoperative recurrence rates were evaluated according to this value. In this way,
the clean surgical margin was determined.
Results The mean age of the participants was 44.77 ± 12.70, and the mean follow-up time was 55.61 ± 16.38 months. Recurrence developed in 5 out of 44 patients (11.4%). The average
recurrence duration was 51 ± 13.87 days. Distance to the average surgical margin was 3.88 ± 0.91 mm. The surgical distances of 5 patients with recurrence were 2, 2.5, 2, 3, and 3 mm,
respectively. It was determined that recurrence was less as the distance (P-DSEM) from the tissue to the surgical excision margin increased (p = 0.001).
Conclusions We found that the recurrence rate in pterygium surgery was linked to the clean surgical margin. When planning pterygium surgery, we believe that determining the amount of
tissue to be excised before surgery will reduce recurrence rates.
Eintrübung hydrophiler Acrylintraokularlinsen: Überblick zu Labormethoden der histologischen Analyse und Replikation der Kalzifikation
Authors: Britz Leoni , Schickhardt Sonja Katrin , Auffarth Gerd U. , Khoramnia Ramin 
Published: June 2023

ZusammenfassungEintrübungen von Acryllinsen durch Materialveränderungen stellen schwerwiegende Komplikationen dar, welche die sehr guten postoperativen Visusergebnisse unkomplizierter Kataraktoperationen
gefährden. Für hydrophobe Acryllinsen besteht die Möglichkeit einer Bildung von Glistenings, bei hydrophilen Acryllinsen besteht das Risiko einer Kalzifikation durch Kalziumphosphate. Im
Laufe der Zeit wurden verschiedene Methoden entwickelt, um die Eintrübungen hydrophiler Acryllinsen zu untersuchen. Ziel der vorliegenden Arbeit ist eine Methodenübersicht über die
histologischen Standardfärbungen und Möglichkeiten der Replikation einer IOL-Kalzifikation. Mittels histologischer Färbungen lässt sich die Kalzifikation von Eintrübungen anderer Art
differenzieren und die Ausprägung der Kalzifikation beurteilen. Durch die Entwicklung von In-vivo- und In-vitro-Modellen zur Replikation konnten der Kalzifikation zugrunde liegende
Pathomechanismen identifiziert werden. In-vivo-Modelle eignen sich zur Beurteilung der Biokompatibilität verwendeter Linsenmaterialien. Mithilfe eines In-vitro-Modells in Bioreaktoren kann
die Kinetik der Kristallbildung untersucht werden. Die Verwendung einer Elektrophorese zur Replikation der Kalzifikation unter standardisierten Bedingungen erlaubt einen Vergleich
verschiedener Linsenmaterialien bezüglich des Kalzifikationsrisikos. Die Kombination verschiedener Analyse- und Replikationsmethoden kann in Zukunft dazu verwendet werden, die
Pathomechanismen der Kalziumphosphatbildung und den Zusammenhang zu Risikofaktoren näher zu untersuchen. Dies könnte dazu beitragen, die Kalzifikation hydrophiler Acryllinsen und damit
einhergehende Explantationen und Komplikationen zu vermeiden.
An Old Theme and New Technology: Marked Glucocorticoid-Induced Hyperglycemia in a Hospitalized Patient Uncovered Retrospectively by Her Flash Glucose Monitoring
Authors: Beshyah Salem A. 
Published: June 2023

Abstract
Background Glucocorticoid-induced hyperglycemia is a problem-facing endocrinologists and internal medicine specialists in hospital wards.
Case History A 63-year-old woman with type 2 diabetes was admitted to the hospital with acute exacerbation of chronic obstructive airway disease. She was treated with a short course of intravenous hydrocortisone followed by oral prednisolone. On discharge, she attended her regular diabetes consultation. Throughout the period, she had her flash glucose monitoring (FGM) sensor in place, and she was monitoring her blood glucose regularly. As part of her diabetes clinic routine, the meter data uploaded and ambulatory glucose profiles were examined. These revealed three distinctly different blood glucose levels before, during, and after glucocorticoid therapy. Glucocorticoid therapy resulted in a marked rise in blood glucose that lasted for a further week before it returned to the pre-treatment levels. This old phenomenon has yet to be demonstrated using the new FGM technology.
Conclusions The story (1) asserts the significant impact of glucocorticoid therapy on glycemic control, (2) demonstrates the prolonged impact on glycemic control following discontinuation of glucocorticoids, (3) suggests a lack of adequate monitoring and timely recognition of hyperglycemia in the hospital, and poor management glucocorticoid-induced hyperglycemia either due to failure of conventional monitoring methods or a degree of complacency to appreciate its magnitude.
Hypopituitarism in Adults: Rational Approaches to Diagnosis and Treatment
Authors: AlMalki Mussa H. 
Published: June 2023

AbstractPituitary hormones are responsible for the regulation of growth, development, metabolism, reproduction, and homeostasis. Hypopituitarism is a condition that is defined as partial or complete insufficiency of anterior pituitary hormone secretion, and rarely, posterior pituitary hormone secretion. This condition can result from diseases of the pituitary gland or the hypothalamus. The annual incidence of hypopituitarism has been estimated to be 4.2 per 100,000 yearly, and the prevalence has been estimated at 45.5 per 100,000. The symptoms of hypopituitarism vary. The onset is insidious and depends on the number of hormone deficiencies and their degree of severity. Pituitary hormone deficiency can result in substantial clinical changes that increase the risk of morbidity and mortality. People commonly report persistent symptoms and a decline in their quality of life, both of which can be explained, at least in part, by the inherent shortcomings of hormone replacement strategies in their ability to imitate the normal hormone secretion processes. The diagnosis of hypopituitarism can be straightforward by measuring the lowered basal hormone levels. In cases where the basal hormone levels are uncertain or partial hormone deficiencies have been identified, it may be necessary to perform provocative testing of the hypothalamic–pituitary axis. The hypothalamus and pituitary region can be imaged using magnetic resonance imaging, which provides useful anatomical information. When necessary, genetic studies may be added to the diagnostic approach. The treatment consists of physiological replacement of the individual end-organ hormone deficiencies, and careful monitoring is required throughout the patient's entire life. Individualized hormone replacement therapy that considers potential interactions is recommended. This article provides an overview of the pathophysiology, clinical presentation, general diagnostic guidelines, and treatment options of hypopituitarism.