bluuebunny/crossref_metadata_2025_split huggingface dataset:
Control of the flagellation pattern in
Helicobacter pylori
by FlhF and FlhG
Authors: Gibson Katherine H. , Botting Jack M. , Al-Otaibi Natalie , Maitre Kriti , Bergeron Julien , Starai Vincent J. , Hoover Timothy R. 
Published: September 2023

ABSTRACT

FlhF and FlhG control the location and number of flagella, respectively, in many polar-flagellated bacteria. The roles of FlhF and FlhG are not well characterized in bacteria that have multiple polar flagella, such as
Helicobacter pylori
. Deleting
flhG
in
H. pylori
shifted the flagellation pattern where most cells had approximately four flagella to a wider and more even distribution in flagellar number. As reported in other bacteria, deleting
flhF
in
H. pylori
resulted in reduced motility, hypoflagellation, and the improper localization of flagella to nonpolar sites. Motile variants of
H. pylori
∆
flhF
mutants that had a higher proportion of flagella localizing correctly to the cell pole were isolated, but we were unable to identify the genetic determinants responsible for the increased localization of flagella to the cell pole. One motile variant though produced more flagella than the Δ
flhF
parental strain, which apparently resulted from a missense mutation in
fliF
(encodes the MS ring protein), which changed Asn-255 to aspartate. Recombinant FliF
N255D
, but not recombinant wild-type FliF, formed ordered ring-like assemblies
in vitro
that were ~50 nm wide and displayed the MS ring architecture. We infer from these findings that the FliF
N225D
variant forms the MS ring more effectively
in vivo
in the absence of FlhF than wild-type FliF.

IMPORTANCE

Helicobacter pylori
colonizes the human stomach where it can cause a variety of diseases, including peptic ulcer disease and gastric cancer.
H. pylori
uses flagella for motility, which is required for host colonization. FlhG and FlhF control the flagellation patterns in many bacteria. We found that in
H. pylori
, FlhG ensures that cells have approximately equal number of flagella and FlhF is needed for flagellum assembly and localization. FlhF is proposed to facilitate the assembly of FliF into the MS ring, which is one of the earliest structures formed in flagellum assembly. We identified a FliF variant that assembles the MS ring in the absence of FlhF, which supports the proposed role of FlhF in facilitating MS ring assembly.
Draft genome sequences of 14
Lacticaseibacillus
spp. strains, representatives of a collection of 200 strains
Authors: Colautti Andrea , Rossi Nicolò , Piazza Carla , Comi Giuseppe , Iacumin Lucilla 
Published: September 2023

ABSTRACT
Lactobacilli have a fundamental role in the food industry as starters and probiotics, therefore, requiring special attention concerning food safety. In this work, 14 strains selected accordingly to their genetic fingerprint and physiologic characteristics are presented as representatives of a collection of 200 strains.
Exponential Inequalities for the Tail Probabilities of the Number of Cycles in Generalized Random Graphs
Authors: Bystrov A. A. , Volodko N. V. 
Published: September 2023

Abstract
Let $$R_n
$$ be the centered and normalized number of cycles of
fixed length contained in a generalized random graph with $$n
$$ vertices. We obtain a Höffding-type
exponential inequality for the tail probability of $$R_n$$.
Tracing technological shifts: time-series analysis of correlations between patent classes
Authors: Maragakis M. , Rouni M. A. , Mouza E. , Kanetidis M. , Argyrakis P. 
Published: September 2023

AbstractPatents are used as a reliable indicator for the study of technological evolution in specific fields. Patent citation networks can further enlighten the relation between individual classes of patents that are used to categorize innovation. The tightening or loosening of bonds between a pair of them can point to a changing landscape in either of the two, or in both. It does, however, clearly signal one or more changes. Thus, it is important to point out pairs of classes that undergo processes of this kind, and try to provide plausible explanations for them. We use patent citation data from the European Patent Office to create the time series of all IPC classes. We then examine all pairs of patent classes for correlations, and discuss those which show the greatest increase, or decrease, over time. We identify classes which show both a significant decrease in their correlation with one class and simultaneously an increase with another. We further proceed to check the cross correlations of all pairs in order to identify pairs which show a time lag in following one another. By implementing specific criteria for the selection of the most promising pairs we distinguish some cases which exhibit strong correlation values with time lags of several months (3–10), and for which we can provide a plausible explanation.
Risk Stratification for Early-onset Colorectal Cancer Screening: Are We Ready for Implementation?
Authors: Siegel Erin M. , Ulrich Cornelia M. , Shibata David 
Published: September 2023

AbstractEarly-onset colorectal cancer (EOCRC) is increasing at alarming rates and identifying risk factors is a high priority. There is a need to develop risk stratification approaches for colorectal cancer screening among younger populations. Although there is a growing body of evidence identifying risk factors for EOCRC, including the report by Imperiale and colleagues in this issue, risk stratification for EOCRC screening has not been implemented into practice. This publication highlights how essential it is to bring research findings into practice and bridge the gaps between developing risk prediction modeling in epidemiology and implementation science. While encouraging, we are still a long way off from having a clinically applicable risk prediction tool.See related article by Imperiale et al., p. 513
Investigación narrativa con niños y niñas: una reflexión teórico-metodológica
Authors: Rodríguez-Castrillón Claudia maría , Amador-Baquiro Juan Carlos 
Published: September 2023

(analítico)Se presenta una reflexión teórico-metodológica derivada de una investigación sobre experiencias de participación y subjetividades en niños y niñas, ubicados en contextos institucionales y comunitarios de tres países de la región. En oposición a los enfoques que asumen a estas personas como sujetos receptores per se, la metodología de este estudio adoptó la investigación narrativa, dada su potencialidad para que los participantes logren narrar-se, conocer-se y transformarse. De esta manera, en la primera parte, se propone un acercamiento ontológico, epistémico y metodológico a la investigación narrativa con niños y niñas. Luego, basados en el trabajo de campo del estudio, se abordan técnicas de investigación que favorecen la interacción horizontal para la generación de conocimiento con estas personas, desde lo dialógico y lo estético. Al final, se presentan las conclusiones.
Palabras clave: Niño; niña; infancia; investigación narrativa; relato; técnica. Tesauro de Ciencias Sociales de la Unesco.
Postural Responses to Achilles Tendon Vibration Depend on Feet Positioning
Authors: Teixeira Alves E. , Rinaldin C. D. , Hembecker P. K. , Manffra E. F. , Nadeau S. , Nogueira Neto G. N. , Mesure S. 
Published: September 2023

Mechanical vibration of the Achilles tendon is widely used to analyze the role of proprioception in postural control. The response to this tendon vibration (TV) has been analyzed in the upright posture, but the feet positions have varied in past research. Moreover, investigators have addressed only temporal parameters of the center of pressure (CoP). We investigated the effect of TV on both temporal and spectral characteristics of the CoP motion. Eighteen healthy young adults, stood barefoot, with one foot on each side of a dual platform, wearing glasses with opaque lenses. We applied 20 seconds of Achilles TV (bilaterally with inertial vibrators at a frequency of 80 Hz and an amplitude of .2–.5 mm). We analyzed CoP signals pre-vibration (PRE,4-seconds), during vibration (VIB,20 seconds), and after vibration cessation (REC,20 seconds). We repeated this protocol in natural and standardized positions (15° feet angular opening). For determining CoP amplitude and velocity, we divided the 20 seconds into five phases of four seconds each and calculated spectral parameters for the whole 20-second signals. There was an adaptation process in the speed of the CoP mediolateral ( p < .01) and anteroposterior ( p < .01) and in the displacement of the CoP anteroposterior ( p < .01), with higher values in the VIB condition. Velocity and displacement decreased progressively in the REC condition. Median and peak frequencies were higher in the VIB condition when compared to the REC condition, but only in the mediolateral direction ( p = .01). The standardized foot position led to increased speed in CoP mediolateral, anteroposterior, and mediolateral displacement ( p < .01). CoP spectral characteristics were not affected by foot positioning. We concluded that adaptation of CoP motion in the presence of TV and after its cessation are observable both in time and frequency domains. Feet positioning influenced CoP motion in the presence of TV and after its cessation but it did not affect its spectral characteristics.
Risk and Protective Factors Associated with Suicidal Ideation and Suicide Attempts Among Black Adolescents Based on the 2017 Youth Risk Behavior Survey
Authors: Baiden Philip , LaBrenz Catherine A. , Kim Yi Jin , Muehlenkamp Jennifer J. , Thrasher Shawndaya 
Published: September 2023

The purpose of this cross-sectional study was to investigate risk (violence and victimization, symptoms of depression, substance use, and obesity) and protective factors (physical activity and academic performance) associated with suicidal ideation and suicide attempts among Black adolescents. Data were obtained from the 2017 Youth Risk Behavior Survey. The analytic sample consisted of 658 adolescents ages 14–18 years (51.8% female) who self-identified as Black. Multivariable binary logistic regression was conducted to examine risk and protective factors associated with suicidal ideation and suicide attempts. About 16% of the sample reported suicidal ideation, and 9.1% made a suicide attempt during the past 12 months. Factors associated with suicidal ideation and suicide attempts included weapon-carrying on school property and symptoms of depression. Physical activity ( AOR = 0.28, 95% CI = 0.11–0.68) and higher academic performance had protective effects on suicide attempts ( AOR = 0.35, 95% CI = 0.17–0.71). Clinicians and school counselors working with Black adolescents may want to inquire about suicide when these risk factors are experienced and strengthen the protective factors identified.
Validation of the ACL-RSI Scale in Pediatric and Adolescent Patients
Authors: Cirrincione Peter M. , Gross Preston W. , Matsuzaki Yukiko , Johnson Mitchell A. , Nagra Kiranpreet K. , Green Daniel W. , Fabricant Peter D. 
Published: September 2023

Background: The Anterior Cruciate Ligament–Return to Sport after Injury (ACL-RSI) scale is a 12-item questionnaire assessing psychological readiness to return to sport after anterior cruciate ligament reconstruction. It has been validated for use in adults in multiple languages and in an abbreviated 6-question short form. Additionally, literature has been published using this scale in pediatric and adolescent populations, however it has not yet been validated for use with them. Purpose: To validate the ACL-RSI scale for use with pediatric and adolescent patients. Study Design: Cohort study (Diagnosis); Level of evidence, 2. Methods: Scores of 6- and 12-item ACL-RSI scales for patients undergoing return-to-sport readiness testing 6 to 8 months after anterior cruciate ligament reconstruction were analyzed. Convergent validity testing was performed against the International Knee Documentation Committee (IKDC)/Pediatric IKDC score, Single Assessment Numeric Evaluation (SANE) score, and peak torque asymmetry of knee flexion and extension using Spearman correlations. Discriminant validity testing was performed against age (Spearman correlation), body mass index (Spearman correlation), and sex (Mann-Whitney U test). Reliability testing was performed by calculating Cronbach’s alpha. Floor and ceiling effects were assessed by calculating the number of minimum and maximum scores in the cohort. Results: A total of 51 patients were included in the final analysis. The mean age at surgery was 15.2 ± 2.2 years, and 51.0% were female. The 6- and 12-item ACL-RSI scales demonstrated a strong significant positive correlation with IKDC/Pediatric IKDC scores ( R = 0.723 and 0.717, respectively; P < .001) and moderate significant positive correlation with Single Assessment Numeric Evaluation scores ( R = 0.516 and 0.502, respectively; P < .001) Age at surgery, body mass index, and sex were not correlated with either ACL-RSI scale. Cronbach’s alpha values of the 12- and 6-item ACL-RSI scales in this population were 0.959 and 0.897, respectively. For both the 12- and the 6-item ACL-RSI scales, no floor or ceiling effects were found as the minimum score (0) was not observed in either version, and the maximum score (100) was only observed twice (3.9%) in both versions. Conclusion: The ACL-RSI scale is valid to use with pediatric and adolescent patients. The 6-item scale may be a better choice because it has fewer redundancies and minimizes the risk of questionnaire fatigue.
An Examination of Coping Strategies that Moderated the Effects of COVID-19-Related Stress on Anxiety Among Older Adults in Aotearoa/NZ
Authors: Kaur Navneet , Stephens Christine 
Published: September 2023

Objectives: 1. To describe perceived stress and changes in anxiety and coping among older adults between 2018 and 2020. 2. To examine the moderating roles of social support, exercise, alcohol use, and smoking on the relationship between perceived stress and anxiety. Methods: Longitudinal data collected from 3275 participants ( M = 68.1 years) as part of the NZ Health, Work, and Retirement study’s 2018 and 2020 biennial surveys, were analyzed using hierarchical regression. Results: Older adults perceived COVID-19 as a low-level source of stress. Social support and alcohol use moderated the relationship between perceived mental stress and anxiety. At high-stress levels, only social support exerted a protective effect against the negative impact of stress on anxiety. Discussion: These findings support the general resilience of many older adults and highlight the effectiveness of coping strategies used by those groups who are more likely to be vulnerable to mental stress during a pandemic.
Topical anesthesia versus topical and subconjunctival anesthesia combined in diabetic retinopathy photocoagulation
Authors: Mafrici M. , Fragiotta S. , Tarsitano M.G. , Lorenzi U. , Toscani L. 
Published: September 2023

Purpose comparison between two anesthetic techniques on the ability to reduce pain during panretinal photocoagulation (PRP) treatment. Methods Observational retrospective single center study. Medical charts of patients who underwent PRP for proliferative diabetic retinopathy were revised. Patients were included if they had the first eye treated with oxybuprocaine hydrochloride drops, and in case of severe pain, the fellow eye received topical anesthesia in combination with 2% subconjunctival lidocaine. The groups were compared for pain perception using an analog visual scale (VAS), number of laser spots, number of interruptions, and laser session duration. Results Forty-two eyes of 21 patients (mean age: 58.3 ± 7.6 years) were analyzed. The mean number of laser spots was significantly higher under combined anesthesia (+84.2 ± 155.9 spots, p = 0.01), with a reduced time for laser execution (−2.5 ± 3.12, p = 0.0008). The use of combined anesthesia significantly decreased the number of interruptions (−40.8%, p < 0.0001) into a single session. On the pain grading scale, the pain perception was significantly lower in the combined anesthesia group ( p < 0.0001). In eyes receiving topical anesthesia the treatment was stopped for pain in 5 eyes (23.8%), while 5 eyes under combined anesthesia presented subconjunctival hemorrhage (23.8%). Conclusion Using combined anesthesia in patients subjected to PRP appeared to reduce pain perception limiting the treatment duration and the interruptions for pain without significant complications. Further studies on a larger scale would be desirable to replicate such findings and standardize the analgesic procedures in ophthalmology.
“A case of ocular ischemic syndrome in spontaneous acute internal carotid dissection: The importance of multidisciplinary management”
Authors: Cattaneo Jennifer , Micieli Camilla , Poli Jacopo Carlo , Migliorisi Carmelo , Trabucchi Giuseppe 
Published: September 2023

Aims To emphasize that ocular ischemic syndrome (OIS) is a rare but threatening condition. It is a consequence of occlusion or dissection of internal carotid artery (ICA). Methods A 66-year-old caucasian male presented in the emergency room with painful vision loss in his right eye and mild neurological symptoms. He was diagnosed ocular ischemic syndrome (OIS) in ICA and ophthalmic artery occlusion due to right ICA dissection. Conclusions It is very important to know and be able to recognise OIS as it may often be misdiagnosed or treated as a different entity. Patients with ICA occlusion and OIS must be treated as soon as possible by a stroke unit team to resolve ICA occlusion/dissection and by the ophthalmologist to prevent further ocular related complications.
Retrospective chart review of transplant recipients with cytomegalovirus infection who received maribavir in the Phase 3 SOLSTICE trial: Data at 52 weeks post-maribavir treatment initiation
Authors: Bassel Marielle , Romanus Dorothy , Bo Tien , Sundberg Aimee K , Okala Sandra , Hirji Ishan 
Published: September 2023

Background Cytomegalovirus (CMV) infection is a frequent complication in haematopoietic cell/solid organ transplant (HCT/SOT) recipients. Previous studies report all-cause mortality rates of 31% and 50% in HCT/SOT recipients post-treatment initiation with conventional anti-CMV therapies for refractory or resistant CMV. Methods This was a multi-country, retrospective medical chart review study of HCT/SOT recipients with refractory CMV infection with or without resistance (R/R) who were randomized to the maribavir arm in the open-label Phase 3 SOLSTICE trial. Patients came from 21 SOLSTICE sites across 6 countries; each site randomized ≥3 patients to the maribavir arm. Patients were followed for 52 weeks (SOLSTICE trial period: 20 weeks; follow-up chart review period: 32 weeks). The primary outcomes were mortality and graft status. Results Of 234 patients who were randomized and received maribavir in SOLSTICE, chart abstraction was completed for all 109 patients enrolled across 21 trial sites (SOT, 68/142; HCT, 41/92). At 52 weeks, overall mortality was 15.6% (17/109) and survival probability was 0.84. Among SOT recipients, survival probability was 0.96, and 3 (4.4%) deaths occurred during the chart review period. For the HCT recipients, survival probability was 0.65 with 14 (34.1%) deaths; 8 occurred during SOLSTICE and 6 during the chart review period. No new graft loss or re-transplantation occurred during the chart review period. Conclusions Overall mortality at 52 weeks post-maribavir treatment initiation in this sub-cohort of patients from the SOLSTICE trial was lower than that previously reported for similar populations treated with conventional therapies for R/R cytomegalovirus infection.
Effect of Delayed Endodontic Sealing with Dentin Biomodification on Fracture Resistance of Endodontically Treated Teeth
Authors: Ahmad Javed , Sajjan Girija S. , Panithini Durga Bhavani , Varma Madhu , Kalyan Satish R  , Prasad K Durga 
Published: September 2023

Aim: Endodontically treated teeth display altered tooth structure pertaining to various chemical irrigants and intracanal medicaments. To prevent the compromised adhesive bond, dentin biomodification is indicated. This study was designed to evaluate the influence of dentin biomodification on the fracture resistance of root canal-treated teeth following Delayed Endodontic Sealing (DES). Materials and Methods: In 50 maxillary central incisors, Class III cavities along with access cavities were prepared. The samples were randomly divided into five groups. Group 1: Intact teeth ( N = 10), Group 2: Immediate Endodontic Sealing (IES) ( N = 10); Group 3: DES after irrigation with 3% NaOCl ( N = 10); Group 4: DES with 2% chlorhexidine (CHX) as a biomodifier ( N = 10) and Group 5: DES with 5% grape seed extract (GSE) as a biomodifier ( N = 10). In Group 2 samples, composite restorations were done prior to irrigation with 3% sodium hypochlorite (NaOCl). Group 3 samples received root canal irrigation with 3% NaOCl for 1 min, etch and rinse procedure and composite restorations. In Groups 4 and 5, dentin biomodification was done prior to composite restorations. Teeth were subjected to fracture resistance tests. Statistical analysis was carried out employing analysis of variance (ANOVA) and Tukey’s post hoc tests ( p < .05). Results: IES group demonstrated the highest fracture resistance, with a significant difference statistically ( p < .05) when compared with the DES groups. DES modified with GSE resulted in significantly superior fracture resistance compared to DES only, but no statistical difference was present between the CHX and grape seed-treated DES groups. Conclusion: DES with the use of dentin biomodifiers such as 5% GSE and 2% CHX increased the fracture resistance of root canal-treated teeth compared to DES only.
Hierarchical CNNPID Based Active Steering Control Method for Intelligent Vehicle Facing Emergency Lane-Changing
Authors: Wang Wensa , Liang Jun , Pan Chaofeng , Chen Long 
Published: September 2023

AbstractTo resolve the response delay and overshoot problems of intelligent vehicles facing emergency lane-changing due to proportional-integral-differential (PID) parameter variation, an active steering control method based on Convolutional Neural Network and PID (CNNPID) algorithm is constructed. First, a steering control model based on normal distribution probability function, steady constant radius steering, and instantaneous lane-change-based active for straight and curved roads is established. Second, based on the active steering control model, a three-dimensional constraint-based fifth-order polynomial equation lane-change path is designed to address the stability problem with supersaturation and sideslip due to emergency lane changing. In addition, a hierarchical CNNPID Controller is constructed which includes two layers to avoid collisions facing emergency lane changing, namely, the lane change path tracking PID control layer and the CNN control performance optimization layer. The scaled conjugate gradient backpropagation-based forward propagation control law is designed to optimize the PID control performance based on input parameters, and the elastic backpropagation-based module is adopted for weight correction. Finally, comparison studies and simulation/real vehicle test results are presented to demonstrate the effectiveness, significance, and advantages of the proposed controller.