bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implications of the European gas market for the long‐term construction of underground gas storage in China
Authors: Du Xueping , Liu Menglin , Qiu Shuo , Wu Jiangtao , Meng Xianyang 
Published: March 2023

AbstractUnderground gas storage (UGS) is an important part of the natural gas industry. Its peaking characteristics and storage capacity are critical to national energy supply and energy security. Compared with the mature gas market in Europe, the construction of UGSs in China faces many challenges. This study is expected to provide references for China UGS through a detailed analysis and calculations of the European natural gas market and underground storage. First, the current status and development of the European gas market were summarized. And the situation of primary energy structure, gas utilization, and UGSs construction was compared between the EU and China. Second, the working gas volume of UGSs in Europe was analyzed from the perspective of its relationship with total consumption and peak‐shaving volume. Finally, the simulation and prediction of the working curve of the energy storage facilities and the reasonable ratio of the energy storage to load were solved by the numerical fitting method. Meanwhile, the recommendations for China's natural gas market and UGSs were provided. It is appropriate to set the planning target of China UGS capacity to load at 5% of total consumption and the actual peaking gas volume will be 14.4 billion cubic meters by 2030.
Arroyo Vega flood relief tunnel design
Authors: Vicente Bruno Andrés 
Published: March 2023

AbstractThis article aims to present structural and geotechnical design challenges of the flood relief project ”Segundo Emisario del Arroyo Vega“, part of Buenos Aires city hydraulic master plan. Works comprised the execution of an 8.4 km‐long tunnel for relief of the existing sewer network. Tunnel alignment develops in a highly populated area, with important interferences such as two metro lines, three railway lines and a main potable water tunnel. All tunnel sections extend mostly within firm, silty soils. Overburden varies between 15 and 25 m. The downstream section was executed with a 6.1 m‐diameter Earth pressure Balance Tunnel Boring Machine (EPB TBM) launched from a 35 m‐diameter shaft with water table pressures up to 200 kPa. Break‐in was performed across a watertight, plastic diaphragm wall cell, which allowed safe shaft wall manual demolition, resulting in a very safe TBM launch. The tunnel upstream section, 2.85 m in diameter, was executed using pipe jacking technology, with the peculiarity of using bidirectional launch shafts along its alignment. To allow water entrance from existing system – through flow diversion chambers – into the new relief tunnel, openings were made in the TBM tunnel, with connection areas of 4 to 7 m2. Its design was particularly challenging, given that unbolted segmental lining was used.
Magnetic resonance evidence of increased iron content in subcortical brain regions in asymptomatic Alzheimer's disease
Authors: Lin Qixiang , Shahid Salman , Hone‐Blanchet Antoine , Huang Shuai , Wu Junjie , Bisht Aditya , Loring David , Goldstein Felicia , Levey Allan , Crosson Bruce , Lah James , Qiu Deqiang 
Published: March 2023

AbstractWhile iron over‐accumulation has been reported in late stage Alzheimer's disease (AD), whether this occurs early in the asymptomatic stage of AD remains unknown. We aimed to assess brain iron levels in asymptomatic AD using quantitative MR relaxometry of effective transverse relaxation rate (R2*) and longitudinal relaxation rate (R1), and recruited 118 participants comprised of three groups including healthy young participants, and cognitively normal older individuals without or with positive AD biomarkers based on cerebrospinal fluid (CSF) proteomics analysis. Compared with the healthy young group, increased R2* was found in widespread cortical and subcortical regions in the older groups. Further, significantly higher levels of R2* were found in the cognitively normal older subjects with positive CSF AD biomarker (i.e., asymptomatic AD) compared with those with negative AD biomarker in subcortical regions including the left and right caudate, left and right putamen, and left and right globus pallidus (p < .05 for all regions), suggesting increased iron content in these regions. Subcortical R2* of some regions was found to significantly correlate with CSF AD biomarkers and neuropsychological assessments of visuospatial functions. In conclusion, R2* could be a valuable biomarker for studying early pathophysiological changes in AD.
Synthesis and Reversible H2 Activation by Coordinatively Unsaturated Rhodium NHC Complexes
Authors: Lutz Marius D. R. , Zhong Hongyu , Trapp Nils , Morandi Bill 
Published: March 2023

AbstractWe report the synthesis of coordinatively unsaturated cationic rhodium complexes bearing the sterically encumbered electron‐rich NHC ligand IPr*OMe. The COD (1,5‐cyclooctadiene) complex [Rh(IPr*OMe)(COD)]BF4 adopts a tilted, pseudo‐square planar coordination geometry, where bonding to the ipso‐carbon of the NHC aryl substituent was observed in the solid state. Hydrogenation of this complex afforded a metastable dihydride complex [Rh(IPr*OMe)(H)2]BF4 with an unusual internal coordination to an arene of the ligand. In the absence of a hydrogen atmosphere, spontaneous reductive elimination of H2 afforded a rhodium complex [Rh(IPr*OMe)]BF4 with a single chelating ligand that stabilizes the highly unsaturated metal by two‐fold π‐face donation as suggested by NMR spectroscopy and computational studies. This unusual complex might serve as a versatile precatalyst for a variety of transformations.
Evaluation of antibiotics resistance in Southern Iran in light of COVID‐19 pandemic: A retrospective observational study
Authors: Raoofi Rahim , Namavari Negin , Rahmanian Vahid , Dousthaghi Mohammad Hadi 
Published: March 2023

AbstractBackground and AimsAntimicrobial resistance (AMR) was taken as one of the high‐priority long‐lasting public health issues, although it might have been underrated in terms of COVID‐19 pandemic emergence. Regarding limited data on assessing the pandemic effect on AMR trend in Iran, this study aimed to describe the epidemiology of antibiotics resistance during the COVID pandemic in southern Iran.MethodsThis descriptive study was conducted on 2675 patients' samples collected and processed in a referral COVID‐19 center hospital in southern Iran from March 21, 2019, to February 18, 2020 (prepandemic), and February 19, 2020, to March 21, 2021 (pandemic). Susceptibility test results in sensitivity and resistance levels were compared in prepandemic and pandemic periods.ResultsCompared to prepandemic, the inpatient number has increased almost three times. On the other hand, there are around four times fewer outpatients now. More than 85% of the specimens were found in urine samples. In all, 92.22% of all bacteria samples were Gram‐negative isolates, with Escherichia coli accounting for 59.19% of them. The change rate of Gram‐negative bacteria resistance to antimicrobials is an average of 7.74% (p < 0.001). On the other hand, the average change rate of Gram‐positive bacteria resistant to antibiotics has decreased by 19.3% (p = 008). As a forerunner among other Gram‐negative bacteria, the average change rate for Pseudomonas aeruginosa and Klebsiella pneumonia resistance to monitored antibiotics was 89% and 66.3%, respectively (p < 0.001).ConclusionDuring the Covid‐19 pandemic, the increase in AMR among Gram‐negative bacteria, particularly P. aeruginosa and K. pneumonia, was observed compared to the prepandemic. This further limits treatment options, and endangers global public health.
Corporate governance, cash holding, and firm performance in an emerging market
Authors: Jiang Yushi , Mubarik Muhammad Shujaat , Zaman Syed Imran , Alam Syed Hasnain , Arif Muhammad 
Published: March 2023

AbstractExtant literature highlights the significance of the corporate governance (CG) mechanism in mitigating agency conflict. Literature also confirms the instrumentality of the board structure (measured as size, diversity, independence and CEO duality) in overcoming the agency problem. In this context, the present study examines the impact of board structure on a firm's cash holding (CH) levels. Likewise, the study tests the role of cash‐holding (CH) in the association between board structure and a firm's performance. Secondary data from 262 PSX‐listed manufacturing and servicing sector firms have been taken for analysis. Panel regression and Generalized Methods of Movement (GMM) were employed to test the modelled relationships. Results show that most of the CG variables, that is, CEO duality, board size, executive compensation, and diversity, do not affect a firm's CH levels in the service and manufacturing sectors. However, the results display a negative and significant impact of block holders (BH), measured as the ratio of the top five shareholders, on cash holding in manufacturing. Likewise, the sector‐wise analysis shows that cash holding significantly mediates between board size and ROA. In contrast, its role between other dimensions of CG and RoA was insignificant. The findings also revealed that firms with frailer CG structures tend to have less CH and can be prone to external environmental risk.
Insights into the binding mechanism between α‐TOH and CYP4F2: A homology modeling, molecular docking, and molecular dynamics simulation study
Authors: Liang Leilei , Zheng Qingchuan 
Published: March 2023

Abstractα‐Tocopherol (α‐TOH) is a potent antioxidant. The concentrations of α‐TOH in plasma are closely related to human health. α‐TOH can be regulated by the metabolism of cytochrome P450 4F2 (CYP4F2). However, the atomic‐level basis for this regulation process remains elusive. Here, we successfully constructed the structure of CYP4F2 by homology modeling and obtained the α‐TOH–CYP4F2 complex models using molecular docking. Three parallel 500 ns molecular dynamics simulations were performed on each complex model to investigate the details of the interaction between α‐TOH and CYP4F2. MM–GBSA method combined with principal component analysis shows that 8 key residues establish a hydrophobic cavity stabilizing α‐TOH in the pocket of CYP4F2 and S423 forms an important hydrogen bond with α‐TOH anchoring α‐TOH in the favorable position for ω‐hydroxylation. Based on our simulation results and the experimental facts, we designed mutation simulation experiments to clarify the important role of two key residues (S423 and V433) in the binding of α‐TOH with CYP4F2. The results show that the mutations directly or indirectly change the binding mode of α‐TOH and decrease its binding affinity with CYP4F2, which is unfavorable for ω‐hydroxylation. Our results could enrich the information on structure‐function relationships of CYP4F2 and provide valuable insights into the regulatory mechanism of CYP4F2 on the metabolism of α‐TOH.
Long noncoding RNAs induced control of ferroptosis: Implications in cancer progression and treatment
Authors: Shi Wei , Sethi Gautam 
Published: March 2023

AbstractA novel kind of nonapoptotic, iron‐dependent cell death brought on by lipid peroxidation is known as ferroptosis. Numerous pathological processes, including neurotoxicity, neurological disorders, ischemia‐reperfusion damage, and particularly cancer, have been demonstrated to be influenced by changes in the ferroptosis‐regulating network. Recent studies have established the critical roles that ferroptosis can play in cancer development and the evolution of resistance to standard chemoradiotherapy, thus suggesting that ferroptosis may be a feasible therapeutic strategy for cancer management. Gene expression may be regulated at the transcriptional and posttranscriptional levels by long noncoding RNAs (lncRNAs). They have been implicated in tumorigenesis. Some lncRNAs participate in the biological process of ferroptosis, which represents an exciting alternative to regulate ferroptosis as a means of cancer therapy. Even though there is evidence that lncRNAs have a mechanistic role in the ferroptosis of cancer cells, research on the mechanism and potential treatments for these lncRNAs is still lacking. We elucidate the potential mechanisms by which lncRNAs modulate ferroptosis in cancer and examine the promise and challenges of employing lncRNAs as novel therapeutic targets in cancer.
HIPK2 mediates M1 polarization of microglial cells via STAT3: A new mechanism of depression‐related neuroinflammation
Authors: Han Chenyang , Pei Hongyan , Sheng Yongjia , Wang Jin , Zhou Xiaohong , Li Wenyan , Zhang Caiqun , Guo Li , Yang Yi 
Published: March 2023

AbstractThis study aimed to investigate the role of protein kinase HIPK2 in depression and its associated mechanism. The chronic unpredictable mild stress (CUSM) model was constructed to simulate mice with depression to detect the mouse behaviors. Moreover, by using mouse microglial cells BV2 as the model. After conditional knockdown of HIPK2, the depressive behavior disorder of mice was improved, meanwhile, neuroinflammation was alleviated, and the M1 cell proportion was reduced. Similar results were obtained after applying the HIPK2 inhibitor tBID or ASO‐HIPK2 treatment. HIPK2 was overexpressed in BV2 cells, which promoted M1 polarization of cells, while tBID suppressed the effect of HIPK2 and reduced the M1 polarized level in BV2 cells. Pull‐down assay results indicated that HIPK2 bound to STAT3 and promoted STAT3 phosphorylation. We found that HIPK2 can bind to STAT3 to promote its phosphorylation, which accelerates M1 polarization of microglial cells, aggravates the depressive neuroinflammation, and leads to abnormal behaviors. HIPK2 is promising as the new therapeutic target of depression.
Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes
Authors: Hua Rui , Wei Jinzi , Torres Mauricio , He Yuxin , Li Yanan , Sun Xiaowei , Wang Li , Inoki Ken , Yoshida Sei 
Published: March 2023

AbstractCircular dorsal ruffles (CDRs) are rounded membrane ruffles induced by growth factors to function as precursors of the large‐scale endocytosis called macropinocytosis. In addition to their role in cellular uptake, recent research using cell line systems has shown that CDRs/macropinocytosis regulate the canonical AKT–mTORC1 growth factor signaling pathway. However, as CDRs have not been observed in tissues, their physiological relevance has remained unclear. Here, utilizing ultrahigh‐resolution scanning electron microscopy, we first report that CDRs are expressed in glomerular podocytes ex vivo and in vivo, and we visually captured the transformation process to macropinocytosis. Moreover, through biochemical and imaging analyses, we show that AKT phosphorylation localized to CDRs upstream of mTORC1 activation in podocyte cell lines and isolated glomeruli. These results demonstrate the physiological role of CDRs as signal platforms for the AKT–mTORC1 pathway in glomerular podocytes at the tissue level. As mTORC1 plays critical roles in podocyte metabolism, and aberrant activation of mTORC1 triggers podocytopathies, our results strongly suggest that targeting CDR formation could represent a potential therapeutic approach for these diseases.
Effective utilization of wheat gluten by lipopeptide biosurfactant in the formulation of cookies
Authors: Ravindran Amrudha , Selvin Joseph , Seghal Kiran George 
Published: March 2023

AbstractBACKGROUNDGluten‐free food products are in demand due to the gluten sensitivity of individuals around the globe. Lipopeptide biosurfactants are widely used in food formulations for wetting, foaming, emulsion stabilization, anti‐adhesive and anti‐microbial properties. Lipopeptide biosurfactants can be used for gluten utilization and the formulation of food products.RESULTSThe strain Bacillus licheniformis MS48 was isolated from the marine sponge associated bacteria and found to be the ideal biosurfactant producer with an emulsification activity of 70%. Biosurfactant production was optimized using lactose, yeast extract, and sodium chloride (NaCl). The 1,1‐diphenyl‐ 2‐picryl hydrazyl (DPPH) radical scavenging activity was found to increase with an increase in the concentration of biosurfactant. The conformation changes in the gluten due to the treatment of lipopeptide biosurfactant was identified by thiol quantification and scanning electron microscopy. With the addition of lipopeptide MS48 to cookie dough, cookies with softer and smooth textures were developed. The conformation changes in the gluten in the lipopeptide incorporated cookie dough was visualized by scanning electron microscopy. The lipopeptide biosurfactant incorporation in cookie dough causes appropriate changes in the structural network of gluten. The lipopeptide‐incorporated cookies had improved the spread factor and texture.CONCLUSIONLipopeptide biosurfactant with effective radical scavenging activity aided in the utilization of gluten. Incorporating lipopeptide in cookie dough assisted in the production of cookies with improved textural and sensory properties. This study is a novel approach to the application of lipopeptide biosurfactant in gluten utilization for the development of cookies. © 2023 Society of Chemical Industry.
Solubilization mechanism of self‐assembled walnut protein nanoparticles and curcumin encapsulation
Authors: Lv Jiao , Zhou Xin , Wang Wenjie , Cheng Yifan , Wang Fengjun 
Published: March 2023

AbstractBACKGROUNDNative walnut protein is an alkali‐soluble protein that seriously limits the application of walnut protein. The pH‐shifting method could improve the solubility of walnut proteins and enable the encapsulation of active ingredients. The present study aimed to prepare water‐soluble nanoparticles of curcumin using walnut protein and evaluate the process of walnut protein self‐assembly, interaction between walnut protein and curcumin, encapsulation properties, and stability of nanoparticles.RESULTSThe solubility of native walnut protein was poor, but the solubility of walnut protein nanoparticles (WPNP) formed by walnut protein after pH‐shifting significantly improved to 91.5 ± 1.2%. This is because, during the process of pH changing from 7 to 12 and back to 7, walnut protein first unfolded under alkaline conditions and then refolded under pH drive, finally forming an internal hydrophobic and external hydrophilic shell‐core structures. The quenching type of walnut protein and curcumin was static quenching, and the quenching constant was 2.0 × 1014 mol−1 L−1 s−1, indicating that the interaction between walnut protein and curcumin was non‐covalent. Adding curcumin resulted in the formation of nanoparticles with small particle size compared with the no‐load. The loading capacity of curcumin‐loaded walnut protein nanoparticles (WPNP‐C) was 222 mg g−1 walnut protein isolate. Under the same mass, the curcumin equivalent concentration in aqueous solution of WPNP‐C was 17 000 times higher than that of the native curcumin.CONCLUSIONThe solubility of the self‐assembled WPNP significantly increased after pH‐shifting treatment. The walnut protein carrier could improve the stability of the encapsulated curcumin. Therefore, walnut proteins could be used as water‐soluble carriers for hydrophobic drugs. © 2023 Society of Chemical Industry.
Chemoinformatic modelling of the antioxidant activity of phenolic compounds
Authors: Idrovo‐Encalada Alondra M , Rojas Ana M , Fissore Eliana N , Tripaldi Piercosimo , Pis Diez Reinaldo , Rojas Cristian 
Published: March 2023

AbstractBACKGROUNDAntioxidants are chemicals used to protect foods from deterioration by neutralizing free radicals and inhibiting the oxidative process. One approach to investigate the antioxidant activity is to develop quantitative structure–activity relationships (QSARs).RESULTSA curated database of 165 structurally heterogeneous phenolic compounds with the Trolox equivalent antioxidant capacity (TEAC) was developed. Molecular geometries were optimized by means of the GFN2‐xTB semiempirical method and diverse molecular descriptors were obtained afterwards. For model development, V‐WSP unsupervised variable reduction was used before performing the genetic algorithms–variable subset selection (GAs‐VSS) to construct the best five‐descriptor multiple linear regression model. The coefficient of determination and the root mean square error were used to measure the performance in calibration (R2 = 0.789 and RMSEC = 0.381), and test set prediction (Q2 = 0.748 and RMSEP = 0.416), along several cross‐validation criteria. To thoroughly understand the TEAC prediction, a fully explained mechanism of action of the descriptors is provided. In addition, the applicability domain of the model defined a theoretical chemical space for reliable predictions of new phenolic compounds.CONCLUSIONThis in silico model conforms to the five principles stated by the Organisation for Economic Co‐operation and Development. The model might be useful for virtual screening of the antioxidant chemical space and for identifying the most potent molecules related to an experimental measurement of TEAC activity. In addition, the model could assist chemists working on computer‐aided drug design for the synthesis of new targets with improved activity and potential uses in food science. © 2023 Society of Chemical Industry.
Impacts of shrubs on soil quality in the native Monte rangelands of Southwestern Buenos Aires, Argentina
Authors: Ambrosino Mariela Lis , Torres Yanina Alejandra , Lucero Cinthia Tamara , Lorda Graciela Susana , Ithurrart Leticia Soledad , Martínez Juan Manuel , Armando Lorena Vanesa , Garayalde Antonio , Busso Carlos Alberto 
Published: March 2023

AbstractShrub cover in semiarid rangelands may induce changes in soil resources and ecosystem functioning. However, it is unknown the real influence that shrub vegetation has on soil quality in rangelands used for livestock purposes. We evaluated the shrub cover effect on 12 chemical and biochemical parameters of soil quality. In a semiarid Monte rangeland of Argentina, 6 paddocks were selected and 10 m transects were placed in a patch with (Sh) and without shrubs (WSh). Then, sites with grasses (Sh‐G and WSh‐G), bare ground‐litter (Sh‐BL and WSh‐BL), and under shrub cover (Sh‐S) were selected. In spring 2017 and 2018, a composite soil sample (0–10 cm in depth) was taken at each site (n = 6). Sh‐G and Sh‐S sites presented high values of soil organic matter, soil organic nitrogen, particulate organic matter (POM), and cellulase activity (CA); WSh‐BL and Sh‐BL sites were associated with the lowest contents of these variables. For the rest of the soil quality parameters, the soil sampling sites showed similar values. These results show that woody presence should not be directly linked to soil quality loss. Although we did not detect a shrub effect in all parameters studied, in the context of appropriate grazing management, the presence of plant species of different functional groups has a positive effect on organic matter and N content of soil close to them. Moreover, in these sites, high POM values represent an important reservoir of potentially available nutrients, and promote CA necessary for fresh litter decomposition improving the soil quality of semiarid rangelands.