bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fleet management system, perceived service quality and the public health sector performance in Zimbabwe
Authors: Chikazhe Lovemore , Siziba Sibonile , Bhebhe Thomas , Sifile Obert , Nyagadza Brighton 
Published: January 2023

PurposeThe purpose of the study is to establish the relationship between public health sector performance (PHSP), fleet management system, perceived service quality (PSQ) and management style (MS).Design/methodology/approachA total of 260 managerial employees were randomly selected from 5 major public hospitals in Zimbabwe to participate in this cross-sectional survey.FindingsFleet management system was found to positively influence both PSQ and PHSP. The results indicated that PSQ has a positive effect on PHSP. MS was found to moderate the effect of fleet management system on both PSQ and PHSP.Originality/valueThe current study provides fresh insights and validates extant knowledge on PHSP, fleet management and PSQ within the public health sector departments. It extends further knowledge on the public health performance in the Sub-Saharan region, as it shows that dimensions on fleet management have a direct influence on PHSP.
Combining corporate environmental sustainability and customer experience management to build an integrated model for decision-making
Authors: Calza Francesco , Sorrentino Annarita , Tutore Ilaria 
Published: January 2023

PurposeThe aim of this work is to provide a theoretical model that can help companies to develop a unique approach to achieve both corporate environmental sustainability (CES) and successful customer experience management (CEM).Design/methodology/approachA two-phase study achieved the research aim. The first phase consisted of the analysis of contemporary theoretical contributions with a focus on CES and CEM. In the second phase, taking a qualitative approach, the key dimensions identified in the initial analysis were investigated to explore the dominant perceptions of practitioners and to hone the theoretical categories.FindingsFive innovative pathways emerged from the study to inform decision-making while maintaining the dual objectives of CES and successful CEM. These pathways are combined to offer a strategic tool for managers and for research advances. This original integrated model also offers six novel theoretical propositions that describe how to shape corporate decisions to achieve environmental sustainability in CEM.Research limitations/implicationsFirms can benefit from an approach that integrates CES and CEM to develop a new mindset for an innovative and valuable decision-making process and to design more captivating experiences for customers. Nevertheless, the efficacy and generalizability of the theoretical framework and propositions require empirical testing.Originality/valueThis paper makes an original contribution to the environmental sustainability and marketing literature by bringing together all elements in these fields of research in a conceptual model. Moreover, this paper proposes theoretical propositions that advance knowledge of the subject and offer ideas for future research and managers.
Co‐design and evaluation of a multidisciplinary teaching resource on mental health recovery involving people with lived experience
Authors: Arblaster Karen , Mackenzie Lynette , Buus Niels , Chen Timothy , Gill Katherine , Gomez Lisa , Hamilton Deborah , Hancock Nicola , McCloughen Andrea , Nicholson Margaret , Quinn Yvette , River Jo , Scanlan Justin Newton , Schneider Carl , Schweizer Richard , Wells Karen 
Published: January 2023

AbstractBackgroundStudents from a range of health disciplines need to learn from people with lived experience of mental distress and recovery to develop recovery capabilities for mental health practice.AimsThe aims of this study are to describe the co‐design of a teaching resource, to explore the experience of people with lived experience during the resource development, and to evaluate the outcome of the resource on student recovery capabilities.MethodUsing a sequential mixed method, a project group consisting of six people with lived experience and 10 academics from five health disciplines was convened to co‐develop teaching resources. People with lived experience met independently without researchers on several occasions to decide on the key topics and met with the research team monthly. The teaching resource was used in mental health subjects for two health professional programmes, and the Capabilities for Recovery‐Oriented Practice Questionnaire (CROP‐Q) was used before and after to measure any change in student recovery capabilities. Scores were compared using the Wilcoxon signed rank test. The people with lived experience were also interviewed about their experience of being involved in constructing the teaching resources. Interviews were audiotaped, transcribed, and analysed thematically.ResultsThe finished resource consisted of 28 short videos and suggested teaching plans. Occupational therapy and nursing student scores on the CROP‐Q prior to using the educational resource (n = 33) were 68 (median) and post scores (n = 28) were 74 (median), indicating a statistically significant improvement in recovery capability (P = 0.04). Lived experience interview themes were (i) the importance of lived experience in education; (ii) personal benefits of participating; (iii) co‐design experience; and (iv) creating the resource.ConclusionCo‐design of teaching resources with people with lived experience was pivotal to the success and quality of the final product, and people with lived experience described personal benefits of participating in resource development. More evidence to demonstrate the use of the CROP‐Q in teaching and practice is needed.
ALKBH5 inhibits the SIRT3/ACC1 axis to regulate fatty acid metabolism via an m6A‐IGF2BP1‐dependent manner in cervical squamous cell carcinoma
Authors: Zhen Lan , Pan Wuyuan 
Published: January 2023

AbstractCervical cancer (CC) is the most common malignancy of the female reproductive system, among which cervical squamous cell carcinoma (CESC) is the most common type. The demethylase ALKBH5 has been previously revealed to be downregulated in CC tissue. N6 methyladenine (m6A) is the most common modification in eukaryotic RNAs and is involved in modulating tumour progression. Therefore, we attempted to clarify the ALKBH5 role and mechanism underlying CESC progression. In CESC, patient tissue and control tissue m6A levels were measured. Reverse transcription quantitative real‐time polymerase chain reaction, western blotting and immunochemistry were used to measure ALKBH5 levels. A correlation between CESC patient survival and ALKBH5 levels was evaluated. Wound healing, transwell and colony formation assays were used to detect CESC cellular behaviours. Corresponding kits and BODIPY staining were used to detect CESC lipid metabolism. Bioinformatics, immunoprecipitation, RNA pulldown and RNA immunoprecipitation assays as well as half‐life measurements were used to assess the association and mechanism of ALKBH5 with silent mating type information regulation 2 homologue 3 (SIRT3), acetyl‐CoA carboxylase 1 (ACC1) and insulin‐like growth factor 2 mRNA binding protein 1 (IGF2BP1). The m6A demethylase ALKBH5 was depleted in CESC tissue and cells, and a low level of ALKBH5 predicted an unfavourable prognosis in CESC patients. ALKBH5 overexpression suppressed CESC growth and lipid metabolism in vitro and CESC tumour growth in vivo, and ACC1 overexpression rescued these changes. ALKBH5 downregulated ACC1 levels in CESC cells by facilitating SIRT3 methylation to repress ACC1 deacetylation. ALKBH5 destabilized SIRT3 to downregulate SIRT3 levels in CESCs in an m6A‐IGF2BP1‐dependent manner. ALKBH5 demethylates and destabilizes SIRT3 in an m6A‐IGF2BP1‐dependent manner, repressing CESC growth, lipid metabolism and tumorigenesis by downregulating ACC1.
Age‐related deficits in neuronal physiology and cognitive function are recapitulated in young mice overexpressing the L‐type calcium channel, CaV1.3
Authors: Moore Shannon J. , Cazares Victor A. , Temme Stephanie J. , Murphy Geoffrey G. 
Published: January 2023

AbstractThe calcium dysregulation hypothesis of brain aging posits that an age‐related increase in neuronal calcium concentration is responsible for alterations in a variety of cellular processes that ultimately result in learning and memory deficits in aged individuals. We previously generated a novel transgenic mouse line, in which expression of the L‐type voltage‐gated calcium, CaV1.3, is increased by ~50% over wild‐type littermates. Here, we show that, in young mice, this increase is sufficient to drive changes in neuronal physiology and cognitive function similar to those observed in aged animals. Specifically, there is an increase in the magnitude of the postburst afterhyperpolarization, a deficit in spatial learning and memory (assessed by the Morris water maze), a deficit in recognition memory (assessed in novel object recognition), and an overgeneralization of fear to novel contexts (assessed by contextual fear conditioning). While overexpression of CaV1.3 recapitulated these key aspects of brain aging, it did not produce alterations in action potential firing rates, basal synaptic communication, or spine number/density. Taken together, these results suggest that increased expression of CaV1.3 in the aged brain is a crucial factor that acts in concert with age‐related changes in other processes to produce the full complement of structural, functional, and behavioral outcomes that are characteristic of aged animals.
Agroecological transition increases arthropod diversity and decreases herbivore abundance on field margins
Authors: Pérez Roig Camila , González Ezequiel , Videla Martin 
Published: January 2023

Abstract
In peri‐urban areas, many farmers are transitioning from conventional agriculture to agroecological practices to reduce pesticide input and preserving ecosystem services such as natural pest control. Field margins represent a stable habitat for arthropods, but community structure depends on many factors, including management type and vegetation features.
We studied the effects of agroecological transitions and vegetation features on arthropods of horticultural field margins, focusing on three feeding guilds (herbivores, predators and parasitoids). We sampled arthropods using the beat‐sheet method in five conventional fields and five under agroecological transition. We also measured vegetation height, richness, flower abundance and plant cover.
Our results showed that arthropod diversity was higher in agroecological fields whereas herbivore abundance was lower, with a consistent pattern across most taxonomic orders. Vegetation features displayed multiple effects among functional and taxonomic groups. Herbivores did not respond to most vegetation variables whereas predators correlated with several, with similar trends among orders.
We conclude that agroecological transitions and field margins with high vegetation richness and floral resources influence arthropod communities with potential benefits regarding pest regulation. These practices might be more effective if considered alongside other methods that enhance biodiversity and if they are consistent at a landscape scale.
Targeting Ara h 2 with human‐derived monoclonal antibodies prevents peanut‐induced anaphylaxis in mice
Authors: Paolucci Marta , Wuillemin Natascha , Homère Valentine , Bieli Dimitri , Köhli Alice , Ballmer‐Weber Barbara , Waeckerle‐Men Ying , Pengo Niccolò , Kündig Thomas M. , Sonati Tiziana , Johansen Pål 
Published: January 2023

AbstractBackgroundPeanut allergy is a type‐I hypersensitivity immune reaction mediated by the binding of peanut allergens to IgE‐FcεRI complexes on mast cells and basophils and by their subsequent cellular degranulation. Of all major peanut allergens, Ara h 2 is considered the most anaphylactic. With few options but allergen avoidance, effective treatment of allergic patients is needed. Passive immunotherapy (herein called PIT) based on prophylactic administration of peanut‐specific monoclonal antibodies (mAbs) may present a promising treatment option for this under‐served disease.MethodFully human recombinant anti‐peanut IgG mAbs were tested in mice sensitized to peanut allergen extract. Allergic mice received intravenous immunotherapy with anti‐peanut Ara h 2‐specific IgG1 or IgG4 mAbs cocktails, and were then challenged by a systemic injection of high‐dose peanut allergen extract. The protection from allergic anaphylaxis was measured by monitoring the core body temperature.ResultsPIT with peanut‐specific mAbs was associated with a significant and dose‐dependent reduction of anaphylactic reactions in peanut‐sensitized mice challenged with peanut allergen extract. Complete protection was observed at doses approximately 0.3–0.6 mg mAbs. Mixtures of mAbs were more effective than single mAbs, and effective treatment could be obtained with mAbs of both IgG1 and IgG4 subclasses. The therapeutic effect of anti‐Ara h 2 mAbs was based on allergen neutralization and independent of the Fcγ receptor and mast‐cell inhibition.ConclusionThis is the first report that shows that human‐derived anti‐peanut mAbs can prevent allergic anaphylaxis in mice. The study demonstrates that neutralizing allergenic epitopes on Ara h 2 by mAbs may represent a promising treatment option in peanut‐allergy.
The lure of an unavailable world: The shifting stakes of contemporary critique
Authors: Pugh Jonathan 
Published: January 2023

AbstractThe lure of an unavailable world is becoming increasingly prominent in Geography and related disciplines. The concern is that much research today remains affirmational—still grasping and instrumentalising being and relation—and that, whilst no doubt modified in such developments as the relational and ontological turns, this nevertheless continues the legacies of the modern episteme in new ways. Indeed, there is a marked momentum, across the social sciences and humanities, from cultural geography to computer and Black studies, to read the reduction of the world to available ontic and ontological cuts and distinctions as a form of violence. In response, tropes of the non‐relational, non‐ontological, the negative, nothingness, the void, absence and the abyss, for examples—what could be called ‘unavailable geographies’—are of growing appeal and interest. This paper, foregrounding the importance of tracking how the material forces of history are read as enabling for the emergence of any new problem space, provides a distinctive pathway into this sense of a critical shift in Western critique. By way of an illustrative example, it focuses upon how the proliferation of logistics (broadly framed here as the logic of obtaining the world by way of cuts and distinctions, from metric culture, to identity politics, to the grasping of ontology and relation) is increasingly understood to open up the power of an undifferentiating reality; one which expands and deepens the unavailable world as a problem space for critique. Thus, whilst geographers, like many others, are currently critiquing dominant approaches for being too affirmational, the key argument of this paper is that we should also be taking one step back, asking why now, and through what broader forces of history, the lure of an unavailable world today?
Autonomy to a fault: The confluence of organ donation, euthanasia, and the dead donor rule
Authors: Rubin Jonah 
Published: January 2023

AbstractFive countries now permit organ donation after euthanasia, on the basis of respecting donor autonomy. Some now openly consider performing euthanasia itself via organ extraction to better preserve organ viability, albeit in violation of the dead donor rule. Proponents argue that respect for patient autonomy requires this option; the dead donor rule is inapplicable since it fulfills donors’ wishes. Other ethical arguments, not addressed herein, explore issues including dying at home, impact on clinicians, and societal faith in donation enterprise, but these concerns are not insurmountable. This paper instead solely critiques proponents’ oversimplified understanding of autonomy with an autonomy‐based argument against any linkage of organ donation and euthanasia, regardless of its timing. Respect for patient autonomy does not unilaterally require fulfilling patients' every wish. Moreover, the very possibility of organ donation with euthanasia limits donor autonomy qualitatively and quantitatively substantially more than by offering it. In fact, organ donation after euthanasia violates the purpose of the dead donor rule, even if not its technicalities.
Why and how do parents decide to adopt? A study on motivations and the decision‐making process in becoming an adoptive family
Authors: Soares Joana , Ralha Sara , Fonseca Sílvia Monteiro , Prego Joana , Barbosa‐Ducharne Maria A. 
Published: January 2023

AbstractThere are several motives underlying the process of deciding to become an adoptive family. However, research exploring this issue is scarce and essentially focused on infertility as the main motivation. The present mixed‐method study aims to fill in this gap by exploring, retrospectively, the motives to adopt of 126 Portuguese adoptive parents. The Parents' Interview on the Adoption Process was used to identify the motives to adopt and describe the adoption decision‐making process. Seven main motives (biological issues, filling in a void/loneliness, adoption as a life project, expanding the family, philanthropy, contact with the child protection system and parenthood) were identified. These motives can be centred on the self, the child or both simultaneously and are characterized by dynamism, permeability and interconnection. Findings allowed for establishing conceptual considerations on motivations to adopt and recommendations for adoption practice.
DNA methylation signature classification of rare disorders using publicly available methylation data
Authors: Hildonen Mathis , Ferilli Marco , Hjortshøj Tina Duelund , Dunø Morten , Risom Lotte , Bak Mads , Ek Jakob , Møller Rikke S. , Ciolfi Andrea , Tartaglia Marco , Tümer Zeynep 
Published: January 2023

AbstractDisease‐specific DNA methylation patterns (DNAm signatures) have been established for an increasing number of genetic disorders and represent a valuable tool for classification of genetic variants of uncertain significance (VUS). Sample size and batch effects are critical issues for establishing DNAm signatures, but their impact on the sensitivity and specificity of an already established DNAm signature has not previously been tested. Here, we assessed whether publicly available DNAm data can be employed to generate a binary machine learning classifier for VUS classification, and used variants in KMT2D, the gene associated with Kabuki syndrome, together with an existing DNAm signature as proof‐of‐concept. Using publicly available methylation data for training, a classifier for KMT2D variants was generated, and individuals with molecularly confirmed Kabuki syndrome and unaffected individuals could be correctly classified. The present study documents the clinical utility of a robust DNAm signature even for few affected individuals, and most importantly, underlines the importance of data sharing for improved diagnosis of rare genetic disorders.
Modeling mammal response to fire based on species’ traits
Authors: Pocknee Christopher A. , Legge Sarah M. , McDonald Jane , Fisher Diana O. 
Published: January 2023

AbstractFire has shaped ecological communities worldwide for millennia, but impacts of fire on individual species are often poorly understood. We performed a meta‐analysis to predict which traits, habitat, or study variables and fire characteristics affect how mammal species respond to fire. We modeled effect sizes of measures of population abundance or occupancy as a function of various combinations of these traits and variables with phylogenetic least squares regression. Nine of 115 modeled species (7.83%) returned statistically significant effect sizes, suggesting most mammals are resilient to fire. The top‐ranked model predicted a negative impact of fire on species with lower reproductive rates, regardless of fire type (estimate = –0.68), a positive impact of burrowing in prescribed fires (estimate = 1.46) but not wildfires, and a positive impact of average fire return interval for wildfires (estimate = 0.93) but not prescribed fires. If a species’ International Union for Conservation of Nature Red List assessment includes fire as a known or possible threat, the species was predicted to respond negatively to wildfire relative to prescribed fire (estimate = –2.84). These findings provide evidence of experts’ abilities to predict whether fire is a threat to a mammal species and the ability of managers to meet the needs of fire‐threatened species through prescribed fire. Where empirical data are lacking, our methods provide a basis for predicting mammal responses to fire and thus can guide conservation actions or interventions in species or communities.
Reporting guidelines enhance the quality and impact of research in Dental Traumatology
Authors: Nagendrababu Venkateshbabu , Duncan Henry F. , Abbott Paul V. , Dummer Paul M. H. 
Published: January 2023

AbstractReporting guidelines assist basic scientists, translational healthcare researchers and clinicians to publish manuscripts of the highest standard by improving the accuracy, transparency and completeness of the publications they submit to journals. This paper provides an overview of reporting guidelines relevant for the specialty of dental traumatology and discusses their application, significance and potential impact. The Preferred Reporting Items for study Designs in Endodontology (PRIDE) suite of reporting guidelines includes a range of study designs that can be used within the broad field of Endodontics but they are also applicable to dental traumatology and other dental disciplines (Preferred Reporting Items for Case reports in Endodontics [PRICE] 2020, Preferred Reporting Items for RAndomized Trials in Endodontics [PRIRATE] 2020; Preferred Reporting Items for Animal Studies in Endodontology [PRIASE] 2021; Preferred Reporting Items for Laboratory studies in Endodontology [PRILE] 2021 and Preferred Reporting items for OBservational studies in Endodontics [PROBE] 2023). The PRIDE guidelines were developed by an extensive network of globally renowned academics, researchers and expert clinicians working within dentistry using an accepted and validated consensus methodology. The aim of the PRIDE guidelines is to improve the overall quality of manuscripts describing case reports, randomized trials, animal research, laboratory studies and observational studies. Although attention to reporting guidelines adds a degree of complexity when writing reports, such guidelines provide a template for authors to develop standardized manuscripts of the highest quality, which will allow colleagues, readers and the wider public to have confidence that their findings are valid and robust. They also provide evidence to editors that manuscripts submitted to journals comply with the highest global standards of reporting within their respective discipline. Endorsement of the PRIDE guidelines by editors will lead to improvements in the reporting quality of manuscripts submitted to their journals.