bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mur Ligase F as a New Target for the Flavonoids Quercitrin, Myricetin, and (-)-Epicatechin
Authors: Hrast Martina , Zdovc Irena , Glavač Nina Kočevar , Gobec Stanislav , Frlan Rok 
Published: July 2023

Abstract
MurC, D, E, and F are ATP-dependent ligases involved in the stepwise assembly of the tetrapeptide stem of forming peptidoglycan. As highly conserved targets found exclusively in bacterial cells, they are of significant interest for antibacterial drug discovery. In this study, we employed a computer-aided molecular design approach to identify potential inhibitors of MurF. A biochemical inhibition assay was conducted, screening twenty-four flavonoids and related compounds against MurC-F, resulting in the identification of quercitrin, myricetin, and (-)-epicatechin as MurF inhibitors with IC50 values of 143 µM, 139 µM, and 92 µM, respectively. Notably, (-)-epicatechin demonstrated mixed type inhibition with ATP and uncompetitive inhibition with D-Ala-D-Ala dipeptide and UM3DAP substrates. Furthermore, in silico analysis using Sitemap and subsequent docking analysis using Glide revealed two plausible binding sites for (-)-epicatechin. The study also investigated the crucial structural features required for activity, with a particular focus on the substitution pattern and hydroxyl group positions, which were found to be important for the activity. The study highlights the significance of computational approaches in targeting essential enzymes involved in bacterial peptidoglycan synthesis.
Genetically predict diet-derived Antioxidants and risk of Neurodegenerative diseases among individuals of European descent: a Mendelian randomization study
Authors: Duan Qing-Qing , Su Wei-Ming , Gu Xiao-Jing , Long Jiang , Jiang Zheng , Yin Kang-Fu , Cai Wei-Chen , Cao Bei , Chi Li-Yi , Gao Xia , Chen Yong-Ping , Li Ju-Rong 
Published: July 2023

Abstract
Background
The prevalence of neurodegenerative disorders (NDDs) is increasing, and there is no cure for any of these diseases. Antioxidants have been associated with several diseases in observational studies. However, the relationship between antioxidants and neurodegenerative diseases remains unclear. The purpose of this study is to use publicly available genome-wide association studies (GWAS) summary statistics to examine the causal relationship between diet-derived antioxidants and the risk of NDDs.
Methods
Single-nucleotide polymorphisms associated with diet-derived antioxidants (vitamin A, vitamin B6, vitamin C, vitamin E, carotene, β-carotene, lycopene, zinc, and selenium) and their metabolites (α-tocopherol, γ-tocopherol, ascorbate, and retinol) were employed as instrumental variables. The NDDs we studied included Parkinson's disease (PD), Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD), which were obtained from GWASs conducted in the European population. Two-sample Mendelian randomization (MR) was performed together with a series of sensitivity analyses. The main statistical analyses were conducted using package “TwoSampleMR (V.0.5.6)” in R (V.4.2.0)
Results
Genetically predicted α-tocopherol led to a lower risk of ALS (OR 0.45; 95% CI, 0.31, 0.66; p = 3.97E-05) based on the random-effect IVW method, and no evidence of heterogeneity and horizontal pleiotropy was found. In addition, we found a potential protective effect of vitamin E and ascorbate on PD, carotene on ALS, and retinol on FTD. However, no circulating antioxidants studied affect the risk for AD.
Conclusion
This study indicated that some diet-derived antioxidants or their metabolites showed evidence of causality in NDDs and provided promising targets for therapeutics.
Postoperative change in the joint-line convergence angle contributes to inaccurate correction in high tibial osteotomy
Authors: Kim Young , Joo Yong Bum , Park Young Cheol , Song Ju-Ho 
Published: July 2023

Abstract
Objective
Accurate correction is a prerequisite for the favorable outcomes of open-wedge high tibial osteotomy (OWHTO). However, previous studies have reported disappointing results regarding correction accuracy despite the use of intra-operative navigation, which implies that a certain factor other than bony components is involved in the inaccurate correction (mainly overcorrection). The joint-line convergence angle (JLCA) can represent soft tissue effects in OWHTO. This study tried to determine whether the postoperative change in the JLCA (∆JLCA) led to inaccurate correction.
Methods
Medical records of 78 OWHTO patients from 2005 to 2021 were retrospectively reviewed. The hip-knee-ankle angle (HKA) was measured with a positive value indicating varus alignment. Inaccurate correction was defined as |postoperative HKA| >3°. The JLCA was measured before and 6 months after surgery on long-standing hip-to-ankle radiographs, and ∆JLCA was defined as the difference between the preoperative and 6-month postoperative JLCAs. ∆JLCA was compared between the accurate correction group and the inaccurate correction group, and a receiver operating characteristic (ROC) curve was used to obtain the cutoff ∆JLCA at which the sensitivity and the specificity for inaccurate correction were maximized. Clinical outcomes were also compared between the groups using the knee injury and osteoarthritis outcome score (KOOS).
Results
Of the 78 patients, inaccurate correction was noted in 10 patients. The overall preoperative and postoperative HKAs were 7.0 ± 3.1° and − 0.4 ± 1.5°, respectively. The accurate correction group and the inaccurate correction group had a difference in ∆JLCA (p = 0.010). However, no significant difference was found in the preoperative HKA (p = 0.529). An ROC curve showed that the cutoff ∆JLCA was 1.9°. In the patients having ∆JLCA ≥ 1.9°, the mean JLCA was 4.9 ± 1.6° preoperatively and 1.7 ± 1.2° postoperatively. In the other patients having ∆JLCA < 1.9°, the mean preoperative and postoperative JLCA were 2.5 ± 1.8° and 2.3 ± 1.8°, respectively. The difference in the preoperative JLCA was significant (p < 0.001). The postoperative KOOS subscales did not differ according to correction accuracy.
Conclusion
Inaccurate correction in OWHTO, specifically valgus overcorrection, is attributable to ∆JLCA which represents the postoperative change of soft tissue effects. Overcorrection should be checked in cases of large preoperative JLCAs.
Absorption changes in Photosystem II in the Soret band region upon the formation of the chlorophyll cation radical [P D1 P D2 ] +
Authors: Boussac Alain , Sugiura Miwa , Nakamura Makoto , Nagao Ryo , Noguchi Takumi , Viola Stefania , Rutherford A. William , Sellés Julien 
Published: July 2023

Abstract
Flash-induced absorption changes in the Soret region arising from the [PD1PD2]+ state, the chlorophyll cation radical formed upon excitation of Photosystem II (PSII), were obtained using Mn-depleted PSII cores at pH 8.6. Under these conditions, TyrD is i) reduced before the first flash, and ii) oxidized before subsequent flashes. In wild-type PSII, when TyrD● is present, an additional signal in the [PD1PD2]+-minus-[PD1PD2] difference spectrum was observed when compared to the first flash when TyrD is not oxidized. The additional feature was “W-shaped” with troughs at 434 nm and 446 nm. This feature was absent when TyrD was reduced, but was present i) when TyrD was physically absent (and replaced by phenylalanine) or ii) when its H-bonding histidine (D2-His190) was physically absent (replaced by a Leucine). Thus, the simple difference spectrum without the double trough feature at 434 nm and 446 nm, seemed to require the native structural environment around the reduced TyrD and its H bonding partners to be present. We found no evidence of involvement of PD1, ChlD1, PheD1, PheD2, TyrZ, and the Cytb559 heme in the W-shaped difference spectrum. However, and surprisingly, the use of a mutant of the PD2 axial His ligand, the D2-His197Ala, shows that the PD2 environment seems involved in the “W-shaped” signal.
m6A reader YTHDF1 inhibits the growth of neuroblastoma in vitro and in vivo
Authors: Deng Jing , Long Jun , Yang Yang , Yang Fengyu 
Published: July 2023

Abstract
Purpose Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, always leading to an unfavorable prognosis. m6A is the most prevalent modification in eukaryotic RNAs. Util now, the role of N6-methyladenosine (m6A) in NB has not been elucidated. In this study, we aim to explore the role of m6A reader YTHDF1 in regulating NB proliferation and growth in vitro and in vivo.Methods We evaluate the role of YTHDF1 in mediating NB cell growth using in vitro and in vivo methods. We perform immunohistochemistry assay to detect the expression of YTHDF1 in NB clinical samples. Cell proliferation assay was used to test the growth of NB cell line. The tumor xenograft mouse model was performed to evaluate the role of YTHDF1 in vivo. RNA-seq was applied to uncover the genes that changed upon YTHDF1 knockdown.Results Our results showed that the expression of YTHDF1 is significantly increased in clinical neuroblastoma samples compared with that of ganglioneuroma (GN) and ganglioneuroblastoma (GNB). Cellular assays demonstrated that knockdown of YTHDF1 inhibits NB cell growth and proliferation. The tumor xenograft mouse model suggests that knockdown of YTHDF1 suppresses the growth of neuroblastoma in vivo. RNA-seq showed that the expression of a series genes was changed upon YTHDF1 knockdown, including ACER3, WNT2B and ANKRD62.Conclusion In conclusion, our study provides a new therapeutic target for the treatment of neuroblastoma via inhibiting YTHDF1.
Reduction of Musculoskeletal Pain in First Postgraduate Year (PGY1) Medicine Residents: A Randomized Controlled Trial on Improving Work Life with Laptop Ergonomics
Authors: Oh Gary Chee Seng , Le Dat , Abugattas Alonso Daniel , Gautam Vivek , Espiritu Neil 
Published: July 2023

Abstract
Background
Work-related musculoskeletal disorders (WRMSDs) are a widely recognized problem among healthcare professionals. First postgraduate year (PGY1) students are particularly at risk of developing WRMSDs due to the demanding nature of their work. This study aimed to evaluate the effectiveness of an ergonomic intervention in reducing musculoskeletal pain among PGY1 and improving their work life.
Methods
This randomized control trial included participants from incoming first-year residents for the academic year 2022–2023. Exclusion criteria included daily medications for headaches, having a history of neck/spine pain injections, history of chronic pain, or already using laptop stands. Data collection utilized a Google Survey form incorporating pain scales at baseline, 3, and 6 months.
Results
Out of 57 potential candidates, 41 residents were eligible. Four subjects were lost due to dropout, transfer, or leaving the residency program, leaving 37 residents by the end. Significant differences (p < 0.05) among control and intervention are shown in shoulder pain, lower back pain, taller subjects with wrist/hand pain, and males with upper back pain. Trends (p < 0.10) existed with heavier subjects, males with upper back and wrist pain and taller subjects with headaches. The other differences in average pain scoring among different treatments, genders, weights, and heights was not statistically significant.
Conclusion
The study findings suggest that the intervention was effective in reducing shoulder, wrist, and lower back pain among residents. However, further research is needed to explore these outcomes.
Bone health in children with Angelman Syndrome at the ENCORE Expertise Center
Authors: Heus Karen G.C.B. Bindels-de , Hagenaar Doesjka A. , Mous Sabine E. , Dekker Ilonka , Kaay Daniëlle C.M. , Kerkhof Gerthe F. , Elgersma Ype , Moll Henriette A. , Wit Marie-Claire Y. 
Published: July 2023

Abstract
Purpose
Angelman Syndrome (AS) is a rare genetic disorder due to lack of UBE3A function on chromosome 15q11.2q13 caused by a deletion, uniparental paternal disomy (UPD), imprinting center disorder (ICD) or pathological variant of the UBE3A gene. AS is characterized by developmental delay, epilepsy, and lack of speech. Although fractures are reported frequently in clinical practice, there are few studies on bone health in AS. The aim of this study is to investigate bone health in children with AS.
Methods
Prospective cohort study of 91 children with AS visiting the ENCORE Expertise Center for AS between April 2010 and December 2021. Bone health was assessed with the Bone Health Index (BHI) in standard deviation score (SDS) measured by digital radiogrammetry of the left hand using BoneXpert software. Risk factors analyzed were age, sex, genetic subtype, epilepsy, anti-seizure medication (ASM) use, mobility, BMI, and onset of puberty.
Results
Children with AS had a mean BHI of -1.77 SDS (SD 1.4). A significantly lower BHI was found in children with a deletion (-2.24 SDS) versus non-deletion (-1.02 SDS). Other factors associated with reduced BHI-SDS were inability to walk and late onset of puberty. Children with a history of one or more fractures (22%) had a significantly lower BHI than children without fractures (-2.60 vs -1.56 SDS). Longitudinal analysis showed a significant decrease in BHI-SDS with age in all genetic subtypes.
Conclusions
Children with AS have a reduced bone health. Risk factors are deletion genotype, no independent walking, and late onset of puberty. Bone health decreased significantly with age.
Towards Controlled Semi-Autonomous Deconstruction
Authors: Lee Hyung Joo , Brell-Cokcan Sigrid 
Published: July 2023

Abstract
The automation of deconstruction processes presents unique difficulties due to the harsh working conditions involved. In this research, we aim to address these limitations by advancing the teleoperated machine, BROKK 170, towards a developed semi-autonomous concept. We propose a framework for robot-assisted deconstruction, exploring the communication and sensing systems of the prototype deconstruction robot, along with its capabilities. Additionally, field tests are conducted to evaluate the performance of the proposed approach in real-world scenarios. The potentials and limitations drawn from these initial results are discussed.
The Unsteady Full Multi-wake Vortex Lattice Method: a full rolled-up detached vorticity approach
Authors: Pimentel-Garcia Jesus Carlos 
Published: July 2023

Abstract
After numerically demonstrating that vorticity is generated on the entire surface for flow past a shell-body through the steady Full Multi-wake Vortex Lattice Method (FMVLM), two unsteady versions are developed. The first one is a straightforward approach, based on the well-known single trailing edge wake Unsteady Vortex Lattice Method (UVLM), but applied to multiple detached wakes, useful during the verification phase; the second one is an improved version of the original one, which enhances results for multi-wake dynamics and aerodynamic coefficients calculations, used during the validation phase. The obtained results allow ensuring that the Unsteady Full Multi-wake Vortex Lattice Method (UFVLM) is well-implemented from a strictly numerical viewpoint and constitutes the cornerstone to future unsteady developments, not only for ideal flow simulations, but also to solve more complex fluid dynamics from a purely Lagrangian description (e.g., through panel-vortex methods).
Young practitioners membership evaluation: A qualitative exploration
Authors: Thomas James 
Published: July 2023

The overarching objectives for this research were to explore the perceptions of newly minted bovine veterinarians regarding the American Association of Bovine Practitioners (the Associa­tion), determine its salient features and benefits, and ascertain if the Association has a non-renewal issue with this population.
This was the first investigation of this sort conducted by the As­sociation with this audience regarding these issues – the area in question was basically unexplored. The information previously available was mostly anecdotal, therefore In-Depth Interviews (IDIs), a qualitative research methodology was employed. A total of 19 IDIs were conducted with bovine veterinarians who had practiced for less than 5 years and had been a member of the American Association of Bovine Practitioners. Thirteen in­dividuals were current members, while 6 respondents were no longer members.
Although there were a number of suggestions for improvement, all respondents pointed out the many positive attributes of AABP and would recommend membership.
Troubleshooting reproductive failures in small ruminants
Authors: Estill Charles T. 
Published: July 2023

As a service to our commercial clients, and with the popularity of “urban agriculture”, large and mixed animal practitioners are frequently called upon to evaluate and treat reproductive disorders of small ruminants. Often, these animals are owned by clients with no or very little previous exposure to animal ag­riculture. In many cases, it is the practitioner’s goal to simply educate these neophyte owners on normal reproductive physi­ology and basic animal husbandry.
Both sheep and goats are inherently very fertile when infec­tious diseases are controlled, proper nutrition provided, and environmental extremes mitigated. Both species typically re­produce very efficiently with little human intervention. Howev­er, as practitioners, we are called upon to investigate reproduc­tive maladies that may be commonplace or, especially as “pet” sheep and goats are kept well past traditional reproductive ages, disorders with which we may be unfamiliar.
This presentation will use a problem list as the basis for dis­cussion of how to troubleshoot a wide range of reproductive disorders of small ruminants. Because of the large depth and breadth of disorders one may encounter, a full discussion of each malady and its treatment is beyond the scope of this pre­sentation. Rather, a series of common client complaints will be listed followed by a discussion of a systematic approach to establishing a diagnosis or etiology. The specifics of treatment will not be discussed.
Modification for Nitration of Halo-substituted meso-Tetraarylporphyrins: A Convenient
Scale-up in Small Amount of Solvent
Authors: Rosa Mariusz , Malinowski Maciej , Ostrowski Stanisław 
Published: July 2023

Abstract:
The synthesis of porphyrins with halogens and nitro groups in meso-aryl rings is
described. Halo-substituted meso-tetraarylporphyrin derivatives in the reaction with sodium
nitrite in trifluoroacetic acid at 40°C (or room temperature) afford 5-(4-nitroaryl)-10,15,20-
triarylporphyrins. The above mono-nitro products bearing halogens on meso-aryl rings (F,
Cl, Br) are difficult to obtain selectively. The method elaborated herein allows to synthesize
them with reasonable yield (of up to 57%), and the reaction can be easily scaled-up. By this
route, the preparation of valuable substrates for further transformation to highly substituted
‘synthetic porphyrins’ was presented.
The Success of Serious Games and Gamified Systems in HIV Prevention and Care: Scoping Review
Authors: Jitmun Waritsara , Palee Patison , Choosri Noppon , Surapunt Tisinee 
Published: July 2023

Background
AIDS, which is caused by HIV, has long been one of the most significant global public health issues. Since the beginning of the HIV epidemic, various types of nonelectronic communication tools have been commonly used in HIV/AIDS prevention and care, but studies that apply the potential of electronic games are still limited.

Objective
We aimed to identify, compare, and describe serious games and gamified systems currently used in HIV/AIDS prevention and care that were studied over a specific period of time.

Methods
A scoping review was conducted into serious games and gamified systems used in HIV prevention and care in various well-known digital libraries from January 2010 to July 2021.

Results
After identifying research papers and completing the article selection process, 49 of the 496 publications met the inclusion criteria and were examined. A total of 32 articles described 22 different serious games, while 17 articles described 13 gamified systems for HIV prevention and care.

Conclusions
Most of the studies described in the publications were conducted in the United States, while only a few studies were performed in sub-Saharan African countries, which have the highest global HIV/AIDS infection rates. Regarding the development platform, the vast majority of HIV/AIDS gaming systems were typically deployed on mobile devices. This study demonstrates the effectiveness of using serious games and gamified systems. Both can improve the efficacy of HIV/AIDS prevention strategies, particularly those that encourage behavior change.
<strong>Type 2 Diabetes Polygenic Score Predicts the Risk of Glucocorticoid-Induced Hyperglycemia in Patients Without Diabetes</strong>
Authors: J. Deutsch Aaron , H. Schroeder Philip , Mandla Ravi , Kang Sarah , Erenler Feyza , M. Mercader Josep , S. Udler Miriam , C. Florez Jose , N. Brenner Laura 
Published: July 2023

<p> </p>
<p>Objective: To assess whether increased genetic risk for type 2 diabetes (T2D) is associated with the development of hyperglycemia after glucocorticoid treatment. </p>
<p>Research Design and Methods: We performed a retrospective analysis of individuals with no diagnosis of diabetes who received a glucocorticoid dose equivalent to 10 mg of prednisone or more. We analyzed the association between hyperglycemia and a T2D global extended polygenic score, which was constructed through a meta-analysis of two published genome-wide association studies. </p>
<p>Results: Out of 546 individuals who received glucocorticoids, 210 developed hyperglycemia and 336 did not. The polygenic score was significantly associated with glucocorticoid-induced hyperglycemia (<em>p</em> = 0.038, OR = 1.4 per standard deviation of the polygenic score). </p>
<p> Conclusions: Individuals with increased genetic risk for T2D have a higher risk of glucocorticoid-induced hyperglycemia. This finding offers a mechanism for risk stratification as part of a precision approach to medical treatment.</p>
Keragaman Bryophyta di Kawasan Wisata Gunung Pandan, Kabupaten Aceh Tamiang, Provinsi Aceh
Authors: Simarmata Putriani , Muhaini Ayuni , Ananda Julia , Lubis Parlindungan , Malau Marturia , Navia Zidni Ilman 
Published: July 2023

Gunung Pandan Tourism is an area with waterfalls and rivers that has the potential to become a Bryophyte habitat. The aim of this study is to look into the diversity of Bryophyte in the Gunung Pandan tourist area. The exploratory method was used to collect data. The mosses discovered during the study were collected, herbarium specimens were made, and the mosses were identified. The results showed up of about 5 Bryophyte species, divided into 4 genera and 4 families. Bryophyte of all varieties can be found on rock, tree trunks, weathered wood, and soil substrates AbstrakWisata Gunung Pandan merupakan suatu kawasan yang berupa air terjun dan sungai sehingga berpotensi menjadi suatu habitat dari lumut. Penelitian ini bertujuan untuk mengkaji keanekaragaman lumut di kawasan wisata gunung pandan. Pengumpulan data dilakukan dengan metode eksplorasi. Lumut yang dijumpai selama penelitian dikoleksi, dibuat spesimen herbarium, dan diidentifikasi. Hasil penelitian diperoleh sebanyak 5 jenis lumut terdiri atas 4 marga dan 4 famili. Semua jenis Bryophyta ditemukan pada tipe substrat batu, batang pohon, kayu lapuk, dan tanah