bluuebunny/crossref_metadata_2025_split huggingface dataset:
B-Cell Responses in Chronic Chagas Disease: Waning of Trypanosoma cruzi–Specific Antibody-Secreting Cells Following Successful Etiological Treatment
Authors: Cesar G , Natale M A , Albareda M C , Alvarez M G , Lococo B , De Rissio A M , Fernandez M , Castro Eiro M D , Bertocchi G , White B E , Zabaleta F , Viotti R , Tarleton R L , Laucella S A 
Published: December 2022

Abstract

Background
A drawback in the treatment of chronic Chagas disease (American trypanosomiasis) is the long time required to achieve complete loss of serological reactivity, the standard for determining treatment efficacy.

Methods
Antibody-secreting cells and memory B cells specific for Trypanosoma cruzi and their degree of differentiation were evaluated in adult and pediatric study participants with chronic Chagas disease before and after etiological treatment.

Results
T. cruzi–specific antibody-secreting cells disappeared from the circulation in benznidazole or nifurtimox-treated participants with declining parasite-specific antibody levels after treatment, whereas B cells in most participants with unaltered antibody levels were low before treatment and did not change after treatment. The timing of the decay in parasite-specific antibody-secreting B cells was similar to that in parasite-specific antibodies, as measured by a Luminex-based assay, but preceded the decay in antibody levels detected by conventional serology. The phenotype of total B cells returned to a noninfection profile after successful treatment.

Conclusions
T. cruzi–specific antibodies in the circulation of chronically T. cruzi–infected study participants likely derive from both antigen-driven plasmablasts, which disappear after successful treatment, and long-lived plasma cells, which persist and account for the low frequency and long course to complete seronegative conversion in successfully treated participants.
Emerging Therapies for Full-Thickness Skin Regeneration
Authors: Palmieri Tina L 
Published: December 2022

Abstract
The classical treatment of extensive full-thickness skin loss due to trauma or burns has been the split-thickness skin graft. While split-thickness skin grafts close the wound, they leave patients with visible scars, dry skin, pruritis, pain, pigmentation alterations, and changes in sensation. The optimal replacement for full-thickness skin loss is replacement with intact full-thickness skin. New technologies combined with advances in the understanding of the mechanisms behind wound healing have led to the development of techniques and products that may eventually recapitulate the functions, appearance, and physical properties of normal skin. Autologous homologous skin constructs, minimal functional skin units, and composite bioengineered skin with dermal substitutes all represent potential avenues for full-thickness composite skin development and application in extensive wounds. This article summarizes the progress, state, and future of full-thickness skin regeneration in burn and massive wound patients.
Advances in Skin Tissue Engineering and Regenerative Medicine
Authors: Jorgensen Adam M , Mahajan Naresh , Atala Anthony , Murphy Sean V 
Published: December 2022

AbstractThere are an estimated 500,000 patients treated with full-thickness wounds in the United States every year. Fire-related burn injuries are among the most common and devastating types of wounds that require advanced clinical treatment. Autologous split-thickness skin grafting is the clinical gold standard for the treatment of large burn wounds. However, skin grafting has several limitations, particularly in large burn wounds, where there may be a limited area of non-wounded skin to use for grafting. Non-cellular dermal substitutes have been developed but have their own challenges; they are expensive to produce, may require immunosuppression depending on design and allogenic cell inclusion. There is a need for more advanced treatments for devastating burns and wounds. This manuscript provides a brief overview of some recent advances in wound care, including the use of advanced biomaterials, cell-based therapies for wound healing, biological skin substitutes, biological scaffolds, spray on skin and skin bioprinting. Finally, we provide insight into the future of wound care and technological areas that need to be addressed to support the development and incorporation of these technologies.
A Risk-Benefit Review of Currently Used Dermal Substitutes for Burn Wounds
Authors: Hill David M , Hickerson William L , Carter Jeffrey E 
Published: December 2022

Abstract
While split-thickness autologous skin grafts remain the most common method of definitive burn wound closure, dermal substitutes have emerged as an attractive option. There are many advantages of utilizing a dermal substitute, notably reducing the need for donor tissue and subsequent iatrogenic creation of a secondary wound. However, there are disadvantages with each that most be weighed and factored into the decision. And most come at a high initial financial cost. There is little comparative literature of the various available and emerging products. This analysis was performed to objectively present risks and benefits of each option.
Use of Dermal Regenerative Templates for Burns
Authors: Mason Stephanie A , Pham Tam N 
Published: December 2022

Abstract
Autologous skin grafting has permitted survival and restoration of function in burn injuries of ever larger total body surface area (TBSA) sizes. However, the goal of replacing “like with like” skin structures is often impossible because full-thickness donor harvesting requires primary closure at the donor site for it to heal. Split-thickness skin grafting (STSG), on the other hand, only harvests part of the dermis at the donor site, allowing it to re-epithelialize on its own. The development of the first dermal regenerative template (DRT) in the late 1970s represented a major advance in tissue engineering that addresses the issue of insufficient dermal replacement when STSGs are applied to the full-thickness defect. This review aims to provide an overview of currently available DRTs in burn management from a clinician’s perspective. It focuses on the main strengths and pitfalls of each product and provides clinical pearls based on clinical experience and evidence.
Surgical Repair of the Acute Burn Wound: Who, When, What Techniques? What Is the Future?
Authors: Leon-Villapalos Jorge , Barret Juan P 
Published: December 2022

AbstractModern burns surgery is multidisciplinary, multimodal and includes a dermal preservation approach. The management of the surgical wound starts in the pre-hospital environment with stabilization and assessment of the burn injured patient according to protocols of trauma resuscitation with special emphasis in the assessment of the burn depth and surface area. A large burn requires fluid resuscitation and physiological support, including counterbalancing hyper metabolism, fighting infection and starting a long burns intensive care journey. A deep burn may impose the need for surgical debridement and cover through a staged approach of excision of devitalized tissue depending on its extension and patient circumstances. These methodologies warrant patients survivability and require professionals integrated in a multidisciplinary team sharing decisions and directing management. Burns Multimodality involves multiple techniques used according to patient’s needs, wound environment, operators experience and available resources. Traditional practices used together with new techniques may reduce morbidity and operative time but also challenge stablished practice. The concept of using the best teams with the best techniques combines with the need for selective and judicious surgery that preserves tissue architecture and spares as much as possible dermal component, therefore reducing the possibility of functional impairment and cosmetic embarrassment caused by pathological scars. Who is best placed to perform these tasks, the appropriate or best timing of surgery and the different practices used to achieve best results will be discussed, together with a reflection on what the future holds for these fundamental steps in the management of the burn injured patient turning into a functional burn survivor.
Mentoring in the USDA Forest Service: A Survey of Aquatic Professionals
Authors: Cerveny Lee , Baral Nabin , Penaluna Brooke , Roper Brett , Shively Daniel , Witt Shelly 
Published: December 2022

AbstractMentoring is suggested as an important strategy to promote workplace inclusivity and is shown to be positively associated with high employee morale, yet mentee needs and experiences may not be universal. To evaluate mentoring impacts from the perspective of USDA Forest Service employees, we conducted an online survey of 251 aquatic professionals, including managers and scientists. 70% of respondents had mentors, and mentorship status did not vary across demographic characteristics. Previous mentoring relationships were most frequently identified as “informal” rather than “formal”; female employees were more likely to desire formal mentoring. Mentored respondents found their work more challenging, fulfilling, and valuable than unmentored respondents. Mentees looked for mentors who could provide constructive feedback, speak candidly, use active listening skills, and who cared about their careers. Overall, respondents were satisfied with their mentors’ skills. Despite strong demand for mentoring, access to mentors among aquatic professionals appears low across all categories.
Desmoid fibromatosis infiltrating left adrenal gland and kidney
Authors: Tee Sui Wu , Richards Avisha , Tan Yi Han , Jeyabalan Dhinisya , Durairaj Gunaseelan 
Published: December 2022

Abstract
Desmoid fibromatosis is a rare, benign, locally aggressive fibroblastic proliferation that may occur in almost any anatomical location. Due to its rarity and unpredictable clinical course, there has not been a standard guideline of treatment. We encountered a case of desmoid fibromatosis in our centre. A young lady previously fit and well was referred for a symptomatic, rapidly growing left sided abdominal mass. Otherwise, she denied any bowel related symptoms or constitutional manifestation. Imaging demonstrated a large well-defined lobulated solid-cystic mass extending from vertebral level T10 to L5, measuring 10.5 cm × 15 cm × 23 cm. The mass was in close proximity with the left adrenal gland, left kidney, pancreas and spleen. Ultrasound guided biopsy interpreted it as a fibroblastic or myelofibroblastic tumour, favouring desmoid fibromatosis. Surgery was then performed where the mass was removed along with the left adrenal gland and kidney. Post-operative care was complicated with pulmonary embolism, hospital-acquired pneumonia and pancreatitis.
The ASAS-SN catalogue of variable stars X: discovery of 116 000 new variable stars using G-band photometry
Authors: Christy C T , Jayasinghe T , Stanek K Z , Kochanek C S , Thompson T A , Shappee B J , Holoien T W-S , Prieto J L , Dong Subo , Giles W 
Published: December 2022

ABSTRACT
The All-Sky Automated Survey for Supernovae (ASAS-SN) is the first optical survey to monitor the entire sky, currently with a cadence of ≲ 24 h down to g ≲ 18.5 mag. ASAS-SN has routinely operated since 2013, collecting ∼ 2 000 to over 7 500 epochs of V- and g-band observations per field to date. This work illustrates the first analysis of ASAS-SN’s newer, deeper, and higher cadence g-band data. From an input source list of ∼55 million isolated sources with g &lt; 18 mag, we identified 1.5 × 106 variable star candidates using a random forest (RF) classifier trained on features derived from Gaia, 2MASS, and AllWISE. Using ASAS-SN g-band light curves, and an updated RF classifier augmented with data from Citizen ASAS-SN, we classified the candidate variables into eight broad variability types. We present a catalogue of ∼116 000 new variable stars with high-classification probabilities, including ∼111 000 periodic variables and ∼5 000 irregular variables. We also recovered ∼263 000 known variable stars.
Perceptions and Reality of Antimicrobial Prescribing During the Transition to Comfort Measures Only at an Academic Medical Center
Authors: Larnard Jeffrey , Blackshear Leslie , Lee Matthew Shou Lun , Buss Mary K , Stead Wendy 
Published: December 2022

Abstract

Background
Little is known about antimicrobial prescribing when patient care is transitioned to comfort measures only (CMO). We used a multidisciplinary survey and retrospective cohort study to gain insight into antimicrobial prescribing in this population at an academic medical center to inform future antimicrobial stewardship interventions.

Methods
A survey focusing on antimicrobial prescribing during the transition to CMO was electronically distributed to providers in medical subspecialities and responses were compared across specialties. A retrospective chart review was performed of patients admitted to an academic medical center in 2020 who were on antimicrobials in the 48 hours prior to CMO. We investigated the percentage of patients who remained on antimicrobials after the transition to CMO and rationale for continuing antimicrobials.

Results
We received 113 survey responses (35% response rate). Forty-one percent of respondents indicated that they “sometimes” or “often” continued antimicrobials during the transition to CMO. Patient/family preference and symptom palliation were the most common factors cited by respondents when deciding whether to continue antimicrobials in this population. Of the 546 patient charts reviewed, 140 (26%) patients were alive 48 hours after CMO order, and 19 (14%) of those patients remained on antimicrobials. Five of 19 (26%) patients had documentation that antimicrobials were continued due to patient/family preference and 5 of 19 (26%) patients had documentation that antimicrobials were continued for palliation of symptoms.

Conclusions
Patient/family preference and symptom palliation are important factors in prescribing antimicrobials when patient care is transitioned to CMO. More evidence is needed regarding palliative benefits of antibiotics to inform provider discussions of benefits and harms of antimicrobial use in this setting.
Do Unjust States Have the Standing to Blame? Three Reservations About Scepticism
Authors: Ewing Benjamin 
Published: December 2022

Abstract
If an offender was treated unjustly by her state in a way that curtailed her options or otherwise made it more likely she would commit her crime, does her state have the moral standing to blame her for that crime? Many theorists have expressed scepticism that the state does. I present three reservations about such scepticism. First, much of it appears to derive its intuitive appeal from problems with the content of state condemnation that can be solved by changing its content rather than its source. Second, adequate legal recognition of everyone’s fundamental moral rights arguably requires not merely the proscription of violations of those rights, but authoritative public condemnation of such violations, which only the state is capable of supplying. Finally, an unjust state would not do disadvantaged offenders any favours by holding its tongue—nor would such offenders help themselves by closing their ears to its criticism.
Bintrafusp Alfa, a Bifunctional Fusion Protein Targeting TGF-β and PD-L1, in Patients with Non-Small Cell Lung Cancer Resistant or Refractory to Immune Checkpoint Inhibitors
Authors: Barlesi Fabrice , Isambert Nicolas , Felip Enriqueta , Cho Byoung Chul , Lee Dae Ho , Peguero Julio , Jerusalem Guy , Penel Nicolas , Saada-Bouzid Esma , Garrido Pilar , Helwig Christoph , Locke George , Ojalvo Laureen S , Gulley James L 
Published: December 2022

AbstractBackgroundBintrafusp alfa is a first-in-class bifunctional fusion protein composed of the extracellular domain of transforming growth factor beta receptor II (a TGF-β “trap”) fused to a human immunoglobulin G1 monoclonal antibody blocking programmed cell death 1 ligand 1 (PD-L1). We report the efficacy and safety in patients with non-small cell lung cancer (NSCLC) that progressed following anti-PD-(L)1 therapy.Materials and MethodsIn this expansion cohort of NCT02517398—a global, open-label, phase I trial—adults with advanced NSCLC that progressed following chemotherapy and was primary refractory or had acquired resistance to anti-PD-(L)1 treatment received intravenous bintrafusp alfa 1200 mg every 2 weeks until confirmed progression, unacceptable toxicity, or trial withdrawal. The primary endpoint was best overall response (by Response Evaluation Criteria in Solid Tumors version 1.1 adjudicated by independent review committee); secondary endpoints included safety.ResultsEighty-three eligible patients (62 [74.7%] treated with ≥3 prior therapies) received bintrafusp alfa. Four patients (3 primary refractory, 1 acquired resistant) had confirmed partial responses (objective response rate, 4.8%; 95% CI, 1.3%-11.9%), and 9 had stable disease. Tumor cell PD-L1 expression was not associated with response. Nineteen patients (22.9%) experienced grade ≥3 treatment-related adverse events, most commonly asthenia (3 [3.6%]) and fatigue, eczema, and pruritus (2 each [2.4%]). One patient had grade 4 amylase increased. One patient died during treatment for pneumonia before initiation of bintrafusp alfa.ConclusionAlthough the primary endpoint was not met, bintrafusp alfa showed some clinical activity and a manageable safety profile in patients with heavily pretreated NSCLC, including prior anti-PD-(L)1 therapy. Tumor responses occurred irrespective of whether disease was primary refractory or had acquired resistance to prior anti-PD-(L)1 therapy.
Weak genetic structure, shared nonbreeding areas, and extensive movement in a declining waterbird
Authors: Shephard Nicholas G , Szczys Patricia , Moore David J , Reudink Matthew W , Costa Jeffrey N , Bracey Annie M , Lisovski Simeon , McKellar Ann E 
Published: December 2022

AbstractUnderstanding population mixing, movements, and connectivity of populations is an important first step towards effective conservation, particularly for long distance migrants that are suffering the greatest population declines, as this allows researchers to recognize how populations may face different risks throughout the annual cycle. We combined population genetic and individual tracking data to quantify the genetic structure and full-cycle movements of the declining North American Black Tern (Chlidonias niger surinamensis). A total of 147 genetic samples were collected from 9 breeding colonies across the range (Maine, Ontario, Michigan, Iowa, Wisconsin, Nebraska, Saskatchewan, and Oregon), and 19 light-level geolocators were recovered from 3 colonies (Ontario, Michigan, and Saskatchewan). Our results demonstrated weak genetic structure, and tracking data demonstrated the use of shared nonbreeding areas between central (Saskatchewan) and eastern (Ontario and Michigan) breeding populations. Our tracking data also provide novel evidence of long-distance breeding dispersal (~1,400 km between breeding locations across years) based on an individual tracked across multiple years, as well as short distance dispersal (~2.5–57 km) based on new recovery locations of 6 tracked individuals. Our results are consistent with the hypothesis that the shared use of nonbreeding areas influences physical condition, timing of departure, and subsequent reproductive timing in such a way as to facilitate dispersal across the breeding range and contribute to weak genetic structure among breeding populations. This study is the first to explore population genetics and migration of North American Black Terns. Extensive movement of individuals may pose a challenge from a conservation perspective as important areas and habitats throughout the annual cycle may be difficult to predict, and future studies should build on our work via extensive mark-resight effort using color bands, tracking individuals from more breeding sites, and examining carry-over effects to further investigate when in the annual cycle populations are most limited.
Organic molecule single-photon sources
Authors: Gaither-Ganim Moses B , Newlon Scott A , Anderson Michael G , Lee Bumsu 
Published: December 2022

Abstract
Since the onset of the 1990s, the development of single-molecule spectroscopy has led to the discovery of various quantum optical signatures in organic single molecules. The single-photon nature of an organic chromophore is of particular importance because of its potential as a nonclassical photon source at room temperature for quantum information science and optical quantum computing. This condensed review focuses on the introduction of fundamental knowledge and basic experimental methods for single-photon sources and organic molecular photophysics and summarizes the research developments in this field, providing an understanding of quantum emission from organic single molecules.
Layer-by-layer assembly of procyanidin and collagen promotes mesenchymal stem cell proliferation and osteogenic differentiation in vitro and in vivo
Authors: Bai Zhibiao , Hu Kai , Shou Zeyu , Yu Jiahuan , Meng Hongming , Zhou Han , Chen Liangyan , Yu Tiantian , Lu Ruofei , Li Na , Chen Chun 
Published: December 2022

Abstract
Collagen, commonly used in tissue engineering, is widespread in various tissues. During bone tissue regeneration, collagen can stimulate the cellular response and determine the fate of cells. In this work, we integrated collagen type II with procyanidin (PC) onto an implant coating by applying a layer-by-layer technique to demonstrate that collagen and PC can participate in the construction of new biomaterials and serve as multifunctional components. The effects of PC/collagen multilayers on the viability of cocultured bone marrow mesenchymal stem cells (BMSCs) were analyzed by cell counting kit-8 analysis and phalloidin staining. The reactive oxygen species level of BMSCs was revealed through immunofluorescent staining and flow cytometry. Osteogenesis-related genes were detected, and in vivo experiment was performed to reveal the effect of newly designed material on the osteogenic differentiation of BMSCs. Our data demonstrated that in BMSCs PC/collagen multilayers accelerated the proliferation and osteogenic differentiation through Wnt/β-catenin signaling pathway and enhanced bone generation around the implant in the bone defect model of rabbit femurs. In summary, combination of collagen and PC provided a new sight for the research and development of implant materials or coatings in the future.