bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current Laboratory Testing Practices for Assessment of ERBB2/HER2 in Endometrial Serous Carcinoma and Colorectal Carcinoma
Authors: Hagemann Ian S. , Bridge Julia A. , Tafe Laura J. , Hameed Meera R. , Moncur Joel T. , Bellizzi Andrew M. , Dolan Michelle , Vasalos Patricia , Kane Megan E. , Souers Rhona J. , Yemelyanova Anna 
Published: December 2022

Context.—
Therapy targeted at human epidermal growth factor receptor 2 (HER2; also known as ERBB2) was used initially for breast and gastroesophageal carcinoma and has more recently been adopted for endometrial serous carcinoma (ESC) and colorectal carcinoma (CRC). There is evidence that predictive biomarker testing algorithms for HER2 must be tumor type specific and that an algorithm validated for one tumor type cannot be applied to another.

Objective.—
To describe current laboratory practices for HER2 assessment in ESC and CRC.

Design.—
We surveyed laboratories participating in the 2021 College of American Pathologists (CAP) HER2 immunohistochemistry proficiency testing program.

Results.—
The survey was distributed to 1548 laboratories and returned by 1195, of which 83.5% (998) were in the United States. For ESC, 24.0% (287) of laboratories reported performing in-house testing for HER2 by immunohistochemical staining and/or in situ hybridization; of these, 44.3% (127) performed it reflexively on all cases of ESC. The most common criterion for evaluating HER2 was the American Society of Clinical Oncology/CAP 2018 guideline for breast carcinoma (69.0%; 194 of 281), whereas only 16.0% (45) of laboratories used guidelines specific to ESC. For CRC, 20.2% (239 of 1185) of laboratories performed in-house HER2 testing, and 82.0% of these (196) did the test only at the clinician’s request. A plurality (49.4%; 115 of 233) used gastroesophageal cancer guidelines when scoring CRC, 30.0% (70) used the CRC scoring system from the HERACLES trial, and 16.3% (38) used the American Society of Clinical Oncology/CAP 2018 guideline for breast carcinoma.

Conclusions.—
Laboratories vary in their approach to HER2 testing in ESC and CRC. Most laboratories did not report using tumor type–specific recommendations for HER2 interpretation. The lack of standardization could present a challenge to evidence-based practice when considering targeted therapy for these diseases.
What motivates Sao Paulo citizens to use bicycles as a mode of transportation? A study in the light of the Goal-Framing Theory
Authors: Fedrigo Foltram Felipe , Bigio Schnaider Paula Sarita , Pelegrina Juliano 
Published: December 2022

Purpose – What motivates Sao Paulo citizens to use bicycles as a mode of transportation? To answer this question, we conceptually rely on the Goal-Framing Theory (Lindenberg, 2000), which seeks to understand how individual choices are made, and exogeneous variables such as climate and temperature which might influence in this decision.
Design/methodology/approach – Empirically, we conduct a quantitative study, relying on primary data from 252 respondents and analyzed by means of logistic regression models, through which we obtained the likelihood of using bikes and the relative weights of each of the variables defined in our theoretical section.
Findings – What motivates Sao Paulo residents are not economic reasons, but rather normative (doing what is right) or hedonic (the pursuit of personal satisfaction) aspects. In other words, we demonstrate that cyclists make this choice to comply with a norm, seeking for benefits to the environment and directly or indirectly to society, as opposed to benefitting individually from this decision.
Practical implications – The research has implication for public policies.
Originality/value – The study is one of the first to empirically test the Goal Framing Theory (Lindenberg, 2000) and, as far as we could tell, the first to assess the motivations for the use of bicycles as a means of transportation in Brazil and in Latin America.
The utility of chest ultrasound-guided fine-needle biopsy in the diagnosis of plasmacytoma
Authors: Benbarka S S , Shubert P T , Irusen E M , Allwood B W , Koegelenberg C F N 
Published: December 2022

Background. Plasmacytoma is a plasma cell dyscrasia originating from a single clone of plasma cells of B-lymphocyte lineage and produces a monoclonal immunoglobulin. Transthoracic fine-needle aspiration (TTNA) under ultrasound (US) guidance is a well-validated technique for the diagnosis of many neoplasms and has been shown to be safe and cost effective, with diagnostic yields comparable to more invasive techniques. However, the role of TTNA in the diagnosis of thoracic plasmacytoma is not well established.
Objective. The aim of this study was to assess the utility of TTNA and cytology in confirming a diagnosis of plasmacytoma.Methods. All cases of plasmacytoma diagnosed from January 2006 to December 2017 by the Division of Pulmonology, Tygerberg Hospital, were retrospectively identified. All patients who underwent an US-guided TTNA and of whose clinical records could be retrieved were included in this cohort. The International Myeloma Working Group’s definition of a plasmacytoma was used as the gold standard. Results. A total of 12 cases of plasmacytoma were identified and 11 patients included (one patient was excluded owing to missing medical records). Six of the 11 patients (mean age 59.5 ± 8.5 years) were male. Radiologically, most had multiple lesions (n=7), most commonly bony (n=6) with vertebral body involvement (n=5) and pleural-based lesions (n=2). Rapid onsite evaluation (ROSE) was performed and documented in 6 of the 11 cases, and a provisional diagnosis of plasmacytoma was suggested in 5 of the 6 patients (83.3%). The final laboratory cytological diagnoses of all 11 cases were compatible with plasmacytoma which was further confirmed via a bone marrow biopsy (n=4) and by serum electrophoresis (n=7).Conclusion. US-guided fine-needle aspiration is feasible and is useful to confirm a diagnosis of plasmacytoma. Its minimally invasive nature may be the ideal investigation of choice in suspected cases.
Criteria for progressive fibrotic hypersensitivity pneumonitis in a Portuguese patient cohort
Authors: Seixas E , Ferreira M , Serra P , Aguiar R , Cunha I , Ferreira P G 
Published: December 2022

Background. Hypersensitivity pneumonitis is a syndrome caused by sensitisation to inhaled antigens that leads to an abnormal immune response in the airways and lung parenchyma. Some patients previously diagnosed with certain types of fibrotic interstitial lung diseases, including fibrotic HP, are susceptible to develop a progressive fibrosing phenotype, despite initial state- of-the-art management.
Objectives. To characterise a cohort of patients with a multidisciplinary diagnosis of chronic f-HP, who were followed up in an ILD outpatient clinic of a hospital in Portugal, and to assess the prevalence of PF-ILD criteria in these patients.Methods. Data were collected from all patients with a definite or provisional diagnosis of f-HP after a multidisciplinary team discussion. Patients were followed up between December 2014 and July 2019. Data included clinical characteristics, high-resolution chest tomography disease patterns, lung function tests, bronchoalveolar lavage and further immunological work-up, biopsy reports, all ILD multidisciplinary team records and diagnostic confidence levels. Patients were assessed according to PF-ILD criteria as defined in the INBUILD trial.
Results. We identified 83 patients with an MTD of HP, who had been followed up for at least 12 months. Of these, 63 (75.9%) were diagnosed with f-HP. Of the 63 f-HP patients, 33.3% (n=21) fulfilled the predefined criteria for PF-HP: 66.7% had a relative decline of ≥10% forced vital capacity; 5% a relative decline of 5 - 9% FVC, with worsening symptoms or increased fibrosis on HRCT; and 23.8% had worsening respiratory symptoms with radiological progression.
Conclusion. This single-centre cohort study demonstrated that a third of f-HP patients presented with PF-ILD, as determined by progression during initial standard-of-care treatment. A usual interstitial pneumonia (UIP)/UIP-like pattern was present in >70% of patients with f-HP, and two-thirds of these patients had an FVC decline of ≥10%.
Position statement for adult and paediatric spirometry in South Africa: 2022 update
Authors: Maree D , Swanepoel R A , Swart F , Gray D M , Masekela R , Allwood B W , Van-Zyl Smit R N , Koegelenberg C F N 
Published: December 2022

Spirometry is required as part of the comprehensive evaluation of both adult and paediatric individuals with suspected or confirmed respiratory diseases and occupational assessments. It is used in the categorisation of impairment, grading of severity, assessment of potential progression and response to interventions. Guidelines for spirometry in South Africa are required to improve the quality, standardisation and usefulness in local respiratory practice. The broad principles of spirometry have remained largely unchanged from previous versions of the South African Spirometry Guidelines; however, minor adjustments have been incorporated from more comprehensive international guidelines, including adoption of the Global Lung Function Initiative 2012 (GLI 2012) spirometry reference equations for the South African population.
All equipment should have proof of validation regarding resolution and consistency of the system. Daily calibration must be performed, and equipment quality control processes adhered to. It is important to have standard operating procedures to ensure consistency and quality and, additionally, strict infection control as highlighted during the COVID-19 pandemic.Adequate spirometry relies on a competent, trained operator, accurate equipment, standardised operating procedures, quality control and patient co-operation. All manoeuvres must be performed strictly according to guidelines, and strict quality assurance methods should be in place, including acceptability criteria (for any given effort) and repeatability (between efforts).
Results must be categorised and graded according to current guidelines, taking into consideration the indication for the test.
Magnetic resonance imaging findings and the clinical characteristics of children with cerebral palsy at a public sector hospital in Gauteng Province, South Africa
Authors: Nel C , Bezuidenhout J K , Thomson H C , Meyer P W A 
Published: December 2022

Background. Cerebral palsy (CP) is a common cause of physical impairment in children. Brain magnetic resonance imaging (MRI) can define different neuropathological patterns of brain injury in CP. There are limited data available on MRI findings of children with CP in Africa.
Objective. To describe the clinical characteristics, risk factors and MRI findings of children with CP attending a developmental clinic at a tertiary hospital in South Africa; and to assess possible associations between the clinical characteristics and pathogenic neuro-imaging patterns.
Methods. This was a retrospective cross-sectional study. The cohort of 112 children was identified from the clinic’s REDcap database. Clinical information was obtained from existing medical records of the patients. Findings from brain MRI reports were classified according to the MRI classification system (MRICS) for CP. The MRI reports were rated independently by two study investigators. A descriptive analysis was conducted.
Results. A total of 112 patient files and MRI brain reports were reviewed. Spastic CP was the most common type of CP (n=75%). The most common perinatal risk factors included prematurity (31%) and low birthweight (28%). Nineteen (17%) children acquired CP after the neonatal period. CP sub-type showed a significant association with functional motor impairment classified as per the gross motor function classification system (GMFCS), p<0.001. Predominant grey matter injury (PGMI) was the most common pathogenic MRI pattern identified (30%). The radiological findings (per MRICS) had a significant association with both the CP sub-type (p<0.005) and functional impairment according to the GMFCS (p<0.001).
Conclusion. Standardised classification of neuro-imaging findings can assist in defining the pathogenesis and clinical manifestations of CP.
Knockdown of hsa_circ_0008922 inhibits the progression of glioma
Authors: Xue Chunhong , Liu Chang , Yun Xiang , Zou Xiaoqiong , Li Xin , Wang Ping , Li Feng , Ge Yingying , Zhang Qingmei , Xie Xiaoxun , Li Xisheng , Luo Bin 
Published: December 2022

Background
A glioma is a tumor originating from glial cells in the central nervous system. Although significant progress has been made in diagnosis and treatment, most high-grade glioma patients are prone to recurrence. Therefore, molecular targeted therapy may become a new direction for adjuvant therapy in glioma. In recent years, many studies have revealed that circular RNA (circRNA) may play an important role in the occurrence and development of many tumors including gliomas. Our previous study found that the expression of hsa_circ_0008922 was up-regulated in glioma tissues upon RNA sequencing. The biological mechanism of circ_0008922 is still unreported in gliomas. Therefore, in this study, we preliminarily outlined the expression of hsa_circ_0008922 in glioma and explored its biological functions.

Methods
The expression of hsa_circ_0008922 in forty glioma tissues and four glioma cell lines (A172, U251, SF763 and U87) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The correlation between hsa_circ_0008922 expression and clinicopathological features of glioma patients was evaluated by Fisher’s exact test. To understand the potential function of hsa_circ_0008922 in glioma, we constructed small interfering RNA (siRNA) to hsa_circ_0008922 to downregulate its expression in glioma cell lines A172 and U251. With these hsa_circ_0008922 downregulated cells, a series of assays were carried out as follows. Cell proliferation was detected by CCK8 assay, migration and invasion were determined by wound healing assay and transwell assay, respectively. Colony formation ability was evaluated by plate clonogenic assay. Moreover, flow cytometry combined with Western blot was performed to analyze apoptosis status and the expression of apoptotic related proteins (caspase 3 and caspase 9). Finally, the possible biological pathways and potential miRNA targets of hsa_circ_0008922 were predicted by bioinformatics.

Results
We found that the expression of hsa_circ_0008922 in glioma tissues was 3.4 times higher than that in normal tissues. The expression of has_circ_0008922 was correlated with WHO tumor grade. After down-regulating the expression of hsa_circ_0008922, malignant biological behavior of glioma cells was inhibited, such as cell proliferation, colony formation, migration, and invasion. At the same time, it also induced apoptosis of glioma cells. Predicted analysis by bioinformatics demonstrated that hsa_circ_0008922 may be involved in tumor-related pathways by acting as a molecular sponge for multiple miRNAs (hsa-let-7e-5p, hsa-miR-506-5p, hsa-let-7b-5p, hsa-let-7c-5p and hsa-let-7a-5p). Finally, we integrated our observation to build a circRNA-miRNA-mRNA predictive network.
Biochemical Screening of Fluted Pumpkin Leaf
Authors: Frances Ejimofor Chiamaka , Enoch Nwakoby Nnamdi , Johnson Oledibe Odira , Eziamaka Afam-Ezeaku Chikaodili , Ann Mbaukwu Onyinye 
Published: December 2022

The well-known vegetable Telfairia occidentalis, sometimes known as the fluted pumpkin leaf, is utilized throughout Nigeria. In Igbo, it is typically referred to as Ugu. The objective of this study was to ascertain the biochemical (phytochemical) makeup of Telfairia occidentalis, a popular vegetable in Nigeria. The study made use of fluted pumpkin leaf extract that was treated differently by soaking in ethanol and water. Alkaloids, flavonoids, glycosides, saponins, tannins, steroids, and phenol compounds were all present in the ethanolic extract at the conclusion of the research, but terpenoids were not, making seven out of the eight phytochemicals present. Alkaloids, flavonoids, steroids, glycosides, and phenol compounds were all present in the water extract, while saponin, tannin, and terpenoids were not. This made five out of the eight phytochemicals present. According to quantitative analysis, the flavonoid concentration in the fluted pumpkin leaf was the highest (11.83 mg/100 g), followed by steroid (11.67 mg/100 g), saponin (4.58 mg/100 g), alkaloid (3.63 mg/100 g), terpenoids (3.56 mg/100 g), and phenol (3.50 mg/100 g), while tannin had the lowest value (0.51 mg/100 g).
Kluver Bucy Syndrome: An Overview of the Clinical Interplay
Authors: Okikiade Adedeji , Osharode Aromedonghene , Aremu Natalie , Chibuike K. Uba , Ogunesan Damisola , Olubunmi Olojede 
Published: December 2022

Kluver Bucy Syndrome (KBS) was initially reported by Sanger Brown and Edward Albert Sharpey-Schafer. In 1939, a bilateral temporal lobectomy was conducted on a Rhesus monkey named Aurora. Three weeks after this procedure, Aurora began to exhibit behavioral changes. These behavioral changes exhibited by Aurora were observed and recorded by Paul Clancy Bucy, a neurosurgeon, and Heinrich Kluver, a neuropsychiatrist; these behavioral changes were given the name Kluver-Bucy syndrome. The first human Kluver-Bucy case was diagnosed in 1955 in a 22-year-old male patient.
Kluver-Bucy syndrome (KBS) is a complex neuropsychiatric disease that usually occurs after bilateral damage to the medial temporal lobes. The syndrome is mainly seen in adults, male and female equally. However, although the syndrome can also be seen in children, the symptoms in children are slightly different from how the symptoms manifest in adults. There are a plethora of etiologies and manifestations of KBS, but it most commonly manifests as a triad of hypersexuality, hyperorality, and hyperphagia.
The diagnosis of KBS is primarily centered around the identification of damages to the medial lobe of the brain using signs and symptoms, imaging studies, and most importantly, Magnetic Resonant Imaging (MRI). It is also essential to rule out and confirm possible differentials and etiologies during the management of KBS. KBS has no known cure, but the various manifestations can be ameliorated with medications like antidepressants and antipsychotics. The prognosis is generally poor for KBS.
Glow Sticks and Other Stories: Children’s Picture Book Revealing Separation Anxiety Disorder (SAD) and Ways to Cope With It
Authors: Hermawan Lidia Dini Tjoa 
Published: December 2022

This journal reveals Separation Anxiety Disorder (SAD) symptoms that may appear during childhood as well as ways children could cope with it in children picture books using realistic fiction as genre and first person point of view. All characters in five stories experience SAD’s symptoms such as irrational worries that something bad will happen to them or to their attachment figures, excessive distress, difficulty sleeping alone, school refusal and even physical complaints such as nausea, headaches and stomachaches caused by unfortunate life events such as loss of loved ones, parental separation or divorce and economic hardships. This journal shows possible ways children may be able to face challenges and overcome their symptoms proactively by using self-directed Cognitive Behavioral Therapy (CBT) such as identifying thought patterns, breaking through negative thoughts, facing fear and putting it all together.
Effect of a Virtual Home-Based Behavioral Intervention on Family Health and Resilience During the COVID-19 Pandemic
Authors: Popescu Filoteia , Sommer Evan C. , Mahoney Margaret R. , Adams Laura E. , Barkin Shari L. 
Published: December 2022

ImportanceVirtual home-based interventions may bolster protective factors, such as family health and resilience, during stressors such as the COVID-19 pandemic; however, their effectiveness is unknown.ObjectiveTo examine the effectiveness of a virtual health coaching intervention on family health and resilience during the pandemic.Design, Setting, and ParticipantsIn this parallel-group, single-site randomized clinical trial, 123 parents and their 2- to 8-year-old children were enrolled at a pediatric clinic or community partner site in Tennessee from March 10 to August 11, 2021. Follow-up surveys were completed between June 29 and November 11, 2021.InterventionsAll participants received 11 weekly cooking videos and associated home-delivered groceries. The intervention group also received 12 weekly, 30-minute virtual health coach sessions.Main Outcomes and MeasuresThe primary outcome was the validated 6-item (range, 6-30) Family Healthy Lifestyle Subscale (FHLS) scores. The secondary outcome was the validated 6-item (range, 0-6) Family Resilience and Connection Index (FRCI) scores. Outcomes were determined a priori and evaluated at baseline and 12-week follow-up. A priori independent t tests and multivariable tobit regression models assessed intervention effects, and post hoc, secondary interaction models assessed whether effects differed over baseline outcomes.ResultsAmong the 123 enrolled families, 110 (89%) were included in the primary analyses (parent mean [SD] age, 35.1 [8.2] years; 104 [95%] female; 55 [50%] non-Hispanic Black; child mean [SD] age, 5.2 [1.7] years; 62 [56%] male). Intervention-control group mean differences were nonsignificant for follow-up FHLS scores (0.7; 95% CI, −0.6 to 2.0; P = .17) and FRCI scores (0.1; 95% CI, −0.5 to 0.6; P = .74). Tobit regression model intervention effects were nonsignificant for FHLS scores (0.9; 95% CI, −0.3 to 2.2; P = .15) and FRCI scores (0.4; 95% CI, −0.2 to 1.1; P = .17). Post hoc, secondary models found no significant interaction for FHLS scores (1.3 increase per 5-point decrease; 95% CI, −0.2 to 2.7; P = .09), with significant intervention associations for baseline scores of 6 to 23. The interaction was significant for FRCI scores (0.4 increase per 1-point decrease; 95% CI, 0.01 to 0.8; P = .047), with significant intervention associations for baseline scores of 0 to 3.Conclusions and RelevanceIn this randomized clinical trial of families with young children, weekly virtual health coaching did not detectably improve family health and resilience. Post hoc, secondary results provided preliminary evidence of potential effectiveness among families with low baseline scores.Trial RegistrationClinicalTrials.gov Identifier: NCT05328193