bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibacterial Activity of Zinc Oxide (ZnO) Biosynthesized from Brown Seaweed Extracts against Pathogenic Bacteria
Authors: Sari Rodiah N. , Utomo Bagus S.B. , Suryaningrum Th. D. , Basmal Jamal , Sedayu Bakti B. , Bardant Teuku B. , Fudholi Ahmad , Zulkifli Sunarto , Wicaksono Arief 
Published: March 2023

Abstract
Nowadays, the main health problem and one of the significant problems in the world is increasing bacterial resistance to antimicrobial agents. Existing research results show that nanoparticle metals could be used to prevent the spread of resistant bacteria in the community. This study was conducted to determine the antibacterial activity of zinc oxide (ZnO) against gram-positive bacteria Staphylococcus aureus and gram-negative bacteria Klebsiella pneumonia and Vibrio parahaemolyticus. ZnO used to be the best ZnO produced from the biosynthesis of brown seaweed (Sargassum sp. and Padina sp.) extracts with Zn(NO3)2.4H2O as precursors at each optimum reaction pH of 10. The diffusion and dilution method used paper discs with three repetitions to conduct the antibacterial activity test. Observations were made by forming a clear zone and the ability to inhibit bacterial growth (MIC). The study's novelty is that ZnO biosynthesized from brown seaweed extract of Sargassum sp. and Padina sp. can inhibit bacterial activity. They can potentially be applied to additional material in fishery product packaging.
Development of diploid embryonic sheep heart cells and sensitivity study of three poxviruses: Lumpy skin disease, Camelpox viruses, and Ecthyma
Authors: Rhazi Halima , Tifrouin Ikram , Mikou Karima , Belayadi Oumaima , Safini Najete , Alhyane Meryem , Tadlaoui Khalid Omari , Lenk Matthias , Elharrak Mehdi 
Published: March 2023

Abstract
Lumpy skin disease virus (LSDV), Camelpox virus (CPV), and Ecthyma virus (ORFV), the three representative viruses of Poxvirus family, are usually grown on embryonated eggs or primary cells for isolation or production since continuous cell lines are less sensitive. The use of eggs or primary cells presents disadvantages such as availability, potential endogenous contaminants, and limited number of passages. In this study, we developed a diploid cell, originated from an embryonic sheep heart and demonstrated their high prolificity and capability of long-term storage. We also demonstrated the maintain of the diploid cell sensitivity to isolation and growth of three genera of Poxvirus family; Capripoxvirus (LSDV), Orthopoxvirus (CPV) and Parapoxvirus (ORFV). Developed cell grow with a doubling time of 24h and can reach 40 passages with a satisfactory yield comparable to Lamb primary testis at passage 5. After infection, the titer of each Poxvirus is maintained between 7, 0 and 7,6 log TCID50/ml up to 5 passages, around 6,8 for the three viruses at passages 6 to 25, 6,4 at passage 30 and 5,6 at passage 40. Sensitivity of diploid embryonic heart cells did not decrease after long-term conservation in liquid nitrogen. Results showed a higher sensitivity of those cells comparatively to lamb primary testis, intensively used for Capripoxvirus and parapoxvirus detection and growth or Vero cells for Orthopoxvirus. The study demonstrated the interest of embryonic heart diploid cells for poxvirus isolation and production to avoid constraints of eggs, embryo, or primary cells.
Forced Intercourse in America: A Pandemic Update
Authors: Axinn William G. , West Brady T. , Schroeder Heather M. 
Published: March 2023

Abstract
Background: Measures of forced intercourse from the U.S. National Center for Health Statistics (NCHS) indicate this a high prevalence among U.S. women that is likely to produce unintended pregnancies. However, NCHS did not measure forced intercourse during the pandemic, limiting knowledge of recent prevalence rates.
Methods: We use multiple nationally-representative, cross-sectional surveys representing the U.S. population from 2011 to 2022 to document these trends. This includes measures from the National Survey of Family Growth, the Panel Study of Income Dynamics Transition into Adulthood Supplement, and the American Family Health Study (AFHS) to provide population estimates of forced intercourse.
Results: Reports of forced intercourse remained high during the pandemic, with more than 25% of U.S. females over 40 reporting lifetime forced intercourse in the AFHS (number of females in AFHS: 1,042). There was a significant increase among females aged 24-28 (p<.05) and rates are highest for those who did not complete college. Among females 24-28, 32.5% (S.E. = 5.7%) with less than 4 years of college reported forced intercourse, a significantly (p<.05) higher rate than among those with higher education.
Conclusions: Rates of forced intercourse among U.S. women remained high during the pandemic, increasing significantly in early adulthood. This exposure to forced intercourse is likely to produce an increase in unintended pregnancies and other sexual, reproductive, and mental health problems.
DNA-functionalized Artificial Chimeric Mechanoreceptor for de novo Force-responsive Cellular Signalling
Authors: Yang Sihui , Wang Miao , Tian Dawei , Zhang Xiaoyu , Cui Kaiqing , Lü Shouqin , Wang Hong-Hui , Long Mian , Nie Zhou 
Published: March 2023

Abstract
Synthetic signalling receptors enable programmable cellular responses coupling with a customized input. However, engineering a designer force-sensing receptor to rewire mechanotransduction remains largely unexplored. Herein, we introduce nongenetically engineered artificial mechanoreceptors (AMRs) capable of reprogramming non-mechanoresponsive receptor tyrosine kinases (RTKs) to sense user-defined force cues, enabling a de novo designed mechanotransduction. AMR is a modular DNA-protein chimera comprising a mechanosensing-and-transmitting DNA nanodevice grafted on natural RTKs via aptameric anchors. AMR senses intercellular tensile force via an allosteric DNA mechano-switch with tuneable piconewton-sensitive force tolerance, actuating a force-triggered dynamic DNA assembly to manipulate RTK dimerization and activate intracellular signalling. By swapping the force-reception ligands, we demonstrate the AMR-mediated activation of c-Met, a representative RTK, in response to the cellular tensile forces mediated by cell-adhesion proteins (integrin, E-cadherin) or membrane protein endocytosis (CI-M6PR). Moreover, the versatility of AMR allows the reprogramming of FGFR1, another RTK, to customize mechanobiological function, e.g., adhesion-mediated neural stem cell maintenance.
Adapting Deep Learning-LSTM method in SDN controller For Secure IoT
Authors: Elsier Omer , MUBARAKALI AZATH , Tayfour Amira Elsir , Marsono Muhammad Nadzir , Hassan Entisar , Abdelrahman Ashraf M. 
Published: March 2023

Abstract
The Internet of Things (IoT) has grown into various enterprise. While the IoT ecosystem's extensive and open environment has many advantages, it can also be a target for a range of growing cyber risks and assaults. The benefits of device integration into a smart ecosystem are enhanced by the IoT's diversity, but the IoT's diverse nature makes establishing a single security solution difficult. However, software-defined networks' (SDNs) centralized intelligence and programmability, it's now possible to put together a single, effective security solution to combat cyber threats and attacks. This study proposes a DL-driven SDN-enabled IoT framework that practice a Deep Neural Network-Long Short-Term Memory (LSTM) classifier to quickly and efficiently detect sophisticated multisector malware botnets. The proposed mechanism was rigorously tested utilizing the most recent state-of-the-art dataset, CICIDS2017, as well as traditional performance evaluation metrics. Furthermore, the proposed technique is compared to current industry norms (i.e., DL algorithms). Extensive testing shows that the proposed method surpasses the competition in terms of detection accuracy while requiring just a minimal compromise in terms of computational cost.
Ultrasound-Guided Lateral Branch Radiofrequency Neurotomy for Sacroiliac Joint Pain After Lumbosacral Spinal Fusion Surgery
Authors: Le Viet-Thang , Nguyen Anh Minh , Do Phuoc Trong 
Published: March 2023

Abstract
Our study is to evaluate the clinical feasibility of the ultrasound-guided lateral branch radiofrequency neurotomy for sacroiliac joint (SIJ) pain after lumbosacral spinal fusion surgery (LSFS). This is a prospective study including a total of 46 patients who were diagnosed with SIJ pain after LSFS, not responding to conservative treatment and therefore underwent ultrasound-guided SIJ radiofrequency ablation (RFA) from Jan 2019 to Jan 2022. These patients were followed up for twelve months after procedure. Patients were assessed with the Numeric Rating Scale (NRS), the Oswestry Disability Index (ODI) pre-procedural and post-procedural for 1-month, 6-month and 12-month follow-ups. There was a significant improvement in post-procedural NRS and ODI scores (p < 0.001). Thirty-eight patients (82.6%) had a satisfactory response, good Global Perceived Effect (GPE) after twelve months. No significant complications were observed for 12 months' follow-up. The ultrasound-guided radiofrequency device designed as a safe, easily applied and encouraging method could avoid a revision surgery. It is a promising technique and has shown good results in providing intermediate pain relief. In addition to the limited series reported in the literature, future studies will add meaning to this topic by including it in routine practice.
Risk factors for long-term invasive mechanical ventilation:a retrospective longitudinal study using German health claims data
Authors: Trudzinski Franziska C , Michels Julia Dorothea , Neetz Benjamin , Meis Jan , Müller Michael , Kempa Axel , Neurohr Claus , Schneider Armin , Felix Herth , Szecsenyi Joachim , Biehler Elena , Fleischhauer Thomas , Wensing Michael , Britsch Simone , Schubert-Haack Janina , Grobe Thomas , Frerk Timm 
Published: March 2023

Abstract
Purpose
Long-term invasive mechanical ventilation (IMV) is a major burden for those affected and causes high costs for the health care system. Early risk assessment is a prerequisite for the best possible support of high-risk patients during the weaning process. We aimed to identify risk factors for long-term IMV within 96 hours after the onset of IMV.
Methods
The analysis was based on data from the AOK Baden-Württemberg; patients who received IMV ≥ 96h and were discharged between 2015 and 2017 were analysed. Health claims data were considered for the previous year and 30 days after hospitalisation. Long-term IMV was defined as evidence of invasive home mechanical ventilation (HMV), IMV≥ 500h, or readmission with (re)prolonged ventilation. The performance of the model was evaluated on a 2018 data-set.
Results
7.584 hospitalisations were analysed. Criteria for long-term IMV were met in 38.3% of cases, of which 13.9% had evidence of HMV, 73.1% were ventilated >500 hours and/or 40.3% were rehospitalised with IMV. Several risk factors could be identified, including pre-existing conditions, admission diagnosis, prescribed aids and procedures. By combining all these factors into a final model, the risk for long-term IMV could be estimated with a sensitivity and specificity of respectively 49 and 80% on the test data set (when classifiying patients based on their predicted probability with a cut-off of 41.15%).
Conclusion
Based on health claims data alone, the risk for long-term IMV could be estimated with an acceptable predictive quality as early as 96 hours after the start of IMV.
Hierarchical self-assembly in colloidal and molecular length scales in thermotropic zwitterionic liquid crystals
Authors: Gustavsson Lotta , Lv Zhong-Peng , Cherian Tomy , Seppälä Wille , Liljeström Ville , Peng Bo , Huotari Simo , Rannou Patrice , Ikkala Olli 
Published: March 2023

Abstract
Hierarchical self-assemblies suggest routes for emerging structural complexities of soft matter towards multifunctional behaviors, however, involving challenges to design the balanced competing attractive and repulsive interactions required for the different length scales. Herein, self-assembly of zwitterionic amphiphiles were explored in the solvent-free state, where especially bis-n-tetradecylphosphobetaine shows temperature-driven reversible transition from low-temperature molecular-scale self-assemblies to high-temperature hierarchical self-assembly, combining colloidal and molecular order. The colloidal order results from layered molecular packing to 2D-nanoplatelets, which form well-defined oblique self-assemblies in several nanometer periodicity, allowing viscous fluidity upon shearing. The colloidal-scale structure emerges due to packing frustration between the tightly packed zwitterionic moieties and alkyl chains, and can be relieved by plasticizing with ionic liquids for purely smectic-A liquid crystallinity. Thus, even molecules with seemingly simple chemical structure can lead to structural hierarchy and tunable complexity by balancing the competing long-range electrostatics and short-range nanosegregations.
Forgotten Joint Score is Worse When the Affected Leg Perceived Longer than Shorter After Total Hip Arthroplasty
Authors: Kawakami Takehiro , Imagama Takashi , Matsuki Yuta , Okazaki Tomoya , Kaneoka Takehiro , Sakai Takashi 
Published: March 2023

Abstract
Background
One of the causes of patient dissatisfaction after total hip arthroplasty (THA) is leg length discrepancy (LLD). Even when radiographic LLD (R-LLD) is within 5 mm, some people perceive the affected side to be longer, while others perceive it is shorter. The purpose of this study was to investigate the relationship between perceived LLD (P-LLD), R-LLD, and Forgotten Joint Score (FJS-12) after THA.
Methods
A retrospective study of 164 patients with unilateral hip disease was conducted. Based on P-LLD after THA, they were classified into three categories: perceived short (PS 21 patients), no LLD (PN 121 patients), and perceived long (PL 22 patients). On the other hand, based on R-LLD after THA, they were divided into <−5 mm (RS 36 patients), −5 mm ≤ x < 5 mm (RN 99 patients), and 5 mm ≥ (RL 29 patients), respectively. The proportion of P-LLD in the RN group was also evaluated. In each group, the relationship between P-LLD, R-LLD and FJS-12 was investigated.
Results
After THA, the PL group had significantly worse FJS-12 (PS: 68.3 ± 26.2, PN: 75.0 ± 20.9, PL: 47.3 ± 25.2, P < .0001). In the R-LLD evaluation, there was no difference in FJS-12 among the three groups(RS: 73.7 ± 21.1, RN: 70.0 ± 24.5, RL: 67.7 ± 25.4, P < .53) . The RN group perceived leg length to be longer (RN-PL) in 12.1% of cases, and the RN-PL groups had significantly worse FJS-12 (RN-PS: 65.4 ± 24.8, RN-PN: 73.8 ± 23.1, RN-PL: 41.8 ± 27.6, P < .0001).
Conclusion
One year after THA, patients with longer P-LLD had worse FJS-12, even if the R-LLD was less than 5 mm.
Alzheimer’s disease brain endothelial-like cells reveal differential drug transporter expression and modulation by potentially therapeutic focused ultrasound
Authors: Chaves Juliana C.S. , Wasielewska Joanna M. , Cuni-Lopez Carla , Rantanen Laura M. , Lee Serine , Koistinaho Jari , White Anthony R. , Oikari Lotta E. 
Published: March 2023

Abstract
Background
The blood-brain barrier (BBB) has a major role in maintaining homeostasis of the brain. The primary function of the BBB is regulating the entry of molecules from the blood to the brain while protecting the brain from potentially harmful endogenous and exogenous substances. Transporters expressed in brain endothelial cells (BECs) mediate the uptake or efflux of compounds to and from the brain and they can also challenge the delivery of drugs into the brain for the treatment of brain disorders including Alzheimer’s disease (AD). AD is the most common form of dementia, however, currently there is limited understanding of the role of BBB transporters in AD-related BBB dysfunction.
Methods
We investigated differences in BBB transporter expression and activity in brain endothelial-like cells (iBECs) differentiated from induced pluripotent stem cells (iPSCs) obtained from people carrying the familial AD Presenilin 1 (PSEN1) mutation (PSEN1 exon 9 deletion; AD-iBECs), healthy controls (Ctrl-iBECs), and isogenic PSEN1 exon 9-corrected lines (PSEN1COR-iBECs). We first characterized the expression levels of 12 BBB transporters in AD-, Ctrl-, and isogenic iBECs to identify any AD specific differences. We then exposed the cells to focused ultrasound, in the absence (FUSonly) or presence of microbubbles (FUS+MB), to examine whether the expression or activity of key transporters can be modulated by therapeutic ultrasound, a novel technique allowing for transient BBB opening.
Results
Our results identified differences in the expression of BBB transporters between AD-iBECs and control iBECs, suggesting disease-specific effects on transporter expression. Additionally, our results demonstrated FUSonly and FUS+MB have the potential to modulate efflux transporter activity. Interestingly, AD-iBECs demonstrated significantly reduced PGP-mediated Aβ accumulation following FUS+MB, an effect not seen in Ctrl-iBECs, suggesting disease-related differences.
Conclusions
Our findings demonstrate that PSEN1 mutant AD-iBECs possess phenotypical differences compared to PSEN1 corrected and unrelated control iBECs in BBB transporter expression and function. Additionally, we show that FUSonly and FUS+MB can modulate the BBB transporter expression and functional activity in iBECs, having potential implications on drug penetration and amyloid clearance. These findings highlight the differential responses of patient cells to FUS treatment, with patient-derived models likely providing an important tool for modelling of therapeutic effects of FUS.
Conversational Agent Dynamics with Minority Opinion and Cognitive Conflict in Small Group Decision-Making
Authors: Nishidai Yuki , Shimojo Shigen , Hayashi Yugo 
Published: March 2023

Abstract
Although studies on group decision-making have greatly contributed to our understanding of group dynamics, it is necessary to further examine the factors that influence group decision-making with conversational agents, such as the agents’ opinions and people’s attitude toward the agents. This study examined the effect of discussion with a group of conversational agents on participants’ decisions. We manipulated the conversational agents’ group opinions and discussion type, and examined individual differences, such as participants’ attitudes toward the agents. In an online experiment, participants read a decision-seeking scenario and responded to the degree of risk they were willing to take. After viewing the opinions of six conversational agents, the participants made a decision again for the same scenario. The results revealed that participants’ decisions shifted toward agents’ opinions regardless of whether they were risky or cautious. Additionally, when the agents’ group opinions were more risk-biased and included a minority opinion, the associations between the degree of a shift to riskier decisions and positive attitude toward the agents were significant. These findings indicate that the agents’ group opinions guided participants toward both risky and cautious decisions and participants’ attitude toward the agents associated with their decision-making, although to a limited extent.
Comprehensive analysis of Major depression Related gene Signature for predicting prognosis in lung adenocarcinoma
Authors: Xue Wenhua , Chen Chengxin , Huang Tao , Cao Jiebin , Duan Xiaoran , Li Chunwei , Yang Yaqi , Li Jun , Deng Jinhai , Peng Mengle , Li Lifeng 
Published: March 2023

Abstract
Background
Lung adenocarcinoma (LUAD) is one of the most common types of cancer in the world, which has attracted much attention due to its high heterogeneity, invasiveness and metastasis. In recent years, depression has been proved to be related to the occurrence and development of various tumors. However, the regulatory pathway of depression related genes on tumor immune microenvironment and how the underlying mechanisms affect the prognosis of LUAD patients remain unclear.
Methods
In this study, we obtained common differentially expressed genes of LUAD patients and MDD patients through R software package "Limma". The relationship between Co-dysregulated genes and metabolism was investigated by KEGG enrichment analysis. According to survival analysis, we screened and constructed Diffgene | Neuropeptides related to prognosis. Finally, the prediction model is constructed using the LASSO algorithm and Cox regression, and its prediction ability is verified.
Results
This study constructed a risk assessment model for LUAD patients based on the 9 neuropeptide genes most related to the prognosis of lung adenocarcinoma, and showed significant predictive effect. The enrichment analysis results of MDD and LUAD Co-dysregulated genes obtained show that the up-regulated genes are enriched in Axon guidance, Ras signaling path, MAPK signaling path, and the down-regulated genes are significantly enriched in Jak-STAT signaling path, Fc epsilon RI signaling path. The results of single cell sequencing data analysis showed that in the high-risk population, it was significantly enriched with ethoxylates, dicarboxylic acid metabolism and pentose phosphate pathway. The low-risk group was more inclined to glycosaminoglycan synthesis, heparin sulfate metabolism and vitamin B6 metabolism.
Conclusions
In general, our study proves for the first time that MDD related gene play an important role in the prognosis of LUAD. The personalized evaluation based on Diffgene | neuropeptide scoring model can accurately predict the prognosis of LUAD patients.
Ethics and Professionalism among Village Health Nurses in Tamil Nadu, India
Authors: Gopichandran Vijayaprasad , Subramaniam Sudharshini , Palanisamy Balasubramanian , Chidambaram Priyadarshini 
Published: March 2023

Abstract
Background: Community Health Workers (CHWs) are the backbone of the public health system in India. They perform several activities at the community level, but little is known about the practice of ethics and professionalism in their work. The CHWs in Tamil Nadu are called Village Health Nurses (VHNs).
Objectives: To explore the experiential wisdom of ethics and professionalism among community health workers in Tamil Nadu.
Methods:We conducted a qualitative study using in-depth interviews and focus group discussions among 125 VHNs in six districts of Tamil Nadu. The data were collected by trained field investigators who were CHWs themselves. Thematic content analysis was done to identify codes, themes and build them into a conceptual model.
Results: The VHNs went beyond the call of their duty to do good to the community. They served as advocates for matters beyond health care in their community. However, overburdened VHNs found it challenging to sustain doing good to the community. Their conceptualization of autonomy ranged from individual autonomous decision making to full paternalistic decision making and this was not uniform across VHNs. The VHNs were sensitive to issues of privacy and confidentiality, but the discussion on these topics were limited. The VHNs reflected the societal norms of gender, class, and caste hierarchies in their work. They had to work amidst difficult power struggles and had their own innovative strategies to subvert power. They faced several ethical conflicts but did not have a standard mechanism for resolving them.
Conclusions: The lack of standard ethical practices among the VHNs indicate that they must be trained in ethics and professionalism and a code of ethics for VHNs is required to help them effectively deliberate on ethical issues during their work.