bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis of liver sinusoidal endothelial cells in patients with liver cirrhosis
Authors: Ma Heming , Liu Xu , Wang Xiaomei , Wu Tong , Wang Guangyi , Lv Guoyue , Niu Junqi 
Published: June 2023

Abstract
Background & Aims: Liver sinusoidal endothelial cells (LSECs) constitute the wall of the hepatic sinusoids, which are the first to be affected by various injuries and play an important role in the occurrence and development of chronic liver diseases caused by different etiologies. Using a combination of bulk RNA sequencing (bulk RNA-seq) and single-cell RNA sequencing (scRNA-seq) techniques, we explored the characteristics of human primary LSECs during liver cirrhosis.
Methods: A single-cell suspension containing human hepatic cells was prepared by circulating collagenase perfusion, and primary LSECs were isolated by density gradient centrifugation and magnetic-activated cell sorting. Immunofluorescence staining was used to identify the isolated LSECs, and cell purity was determined by flow cytometry. scRNA-seq analysis was performed on liver tissues from three patients with a non-cirrhotic liver and four patients with a cirrhotic liver. LSECs were annotated using gene expression patterns, and the differentially expressed genes (DEGs) were compared between the two groups. LSECs from seven patients with a non-cirrhotic liver and four patients with a cirrhotic liver were included in the bulk RNA-seq, and DEGs during liver cirrhosis were analyzed. The results of bulk RNA-seq and scRNA-seq were compared to screen for hub genes with similar trends. Quantitative real-time PCR, western blotting, and tissue immunofluorescence were performed on genes with significant differences to verify dysregulation of transcription and protein expression levels.
Results: A total of 1987 DEGs were identified in bulk RNA-seq, and 461 DEGs were identified in scRNA-seq. A total of 103 common DEGs were identified by combining the two sets of sequencing data. Further screening was performed based on changes in expression levels, and 24 DEGs were retained for additional analysis. There were 19 up-regulated genes (FGF23, CH25, ANGPTL4, IL1RL1, CYP3A7, SERPINE1, F2RL3, MT1M, APOLD1, ADM, MFAP4, ERRFI1, CD9, LCN6, AKAP12, PLIN2, BNIP3, CAV2, and MT1A) and five down-regulated genes (GPR182, HSPA1B, RGS16, HSPA6, and MT-ND4L). Among them, FGF23 mRNA expression was the most up-regulated during cirrhosis. Correspondingly, FGF23 protein levels in LSECs from cirrhotic livers also increased.
Conclusions: Through transcriptome analysis, DEGs related to the pathophysiological processes of LSECs during liver cirrhosis were identified, providing promising targets and pathways for the diagnosis and treatments of liver cirrhosis.
Inverse Magnus Effect Induced by Dilute Water Fog
Authors: Nagahiro Shin-ichiro , Hayakawa Yoshinori 
Published: June 2023

Abstract
The inverse Magnus effect is a phenomenon in which a spinning sphere experiences a lift force opposite to the conventional Magnus effect. This effect is typically observed in the flow higher than a critical Reynolds number or spin ratio. We experimentally studied the inverse Magnus effect on a spinning sphere in a flow containing suspended micrometer size droplets. The lift force was measured by injecting droplets into a closed return wind tunnel. We investigated the threshold of the inverse Magnus effect for several types of dispersed droplets. Our results indicate that the flow with suspended water droplets significantly affects the onset of the inverse Magnus effect, causing a decrease in the critical Reynolds number and spin ratio.
Quantifying the effect of migration status on family planning utilisation in Malawi. Evidence from 2019/20 Malawi Multiple Indicator Cluster Survey
Authors: Moyo Reuben Christopher , Nkhoma Dumisani 
Published: June 2023

Abstract
Background: Family planning (FP) is known to bring multiple benefits to people both individually and collectively. Individually, FP has been associated with reduction in risk of unintended pregnancy which also correlates well with low child mortality rates. Child mortality rates in women with child spacing of less than two years are reported to be 45% higher than their counterparts. Several factors that predict FP utilisation have been investigated but there is limited literature on the effect of migration status on FP utilisation in Malawi. Our study aimed at quantifying the effect of migration status on modern contraceptive use.
Methods: Data for this study came from a nationally representative 2019/20 Malawi multiple cluster indicator survey (MICS). At total of 22,730 women aged 15 to 45 participated in the survey. We applied sampling weights to facilitate survey data analysis to correct unequal representation of participants at cluster, district, and regional level. We used multivariable binary logistic regression to assess the effect of migration status on any modern contraceptive use. The final model had participants age, age at first sex, age at marriage, region, marital status, levels of education, children ever born, residence and wealth index as confounders.
Results: The overall CPR among women aged 15 to 45 was 53.16%. The contraceptive prevalence rates of participants by migration status were 48.38% for migrants and 53.40% for non-migrants. The fully adjusted effect of migration on modern contraceptive use was 0.77 (95% CI 0.67 – 0.91, p=0.001).
Conclusions: Our study concludes that women migrants and adolescent girls have low CPR and are less likely to access modern contraceptives compared to non-migrant women. Deliberate efforts are required to increase CPR for migrants as well as for adolescent girls.
A Neumann problem with supercritical and exponential growth in dimension N ≥ 3
Authors: Khatib Alireza , Moameni Abbas , Mousavinasr Somayeh 
Published: June 2023

Abstract
We will consider the following problem:
-Δu + u = f(u),
x ∈ Ω, u > 0, x ∈ Ω,
∂u/∂ν = 0, x ∈ ∂Ω,
where Ω is a bounded annulus in ℝ^N (N ≥ 3), and the function f has either exponential growth given by the Trudinger-Moser inequality f(u) = u(e^{u^2}-1), or it is of the power form f(u) = u|u|^{p-2}, where p is supercritical. It is known that this problem always has a positive radial solution. Our main goal in this paper is to prove the existence of a positive non-radial solution for the case f(u) = u(e^{u^2}-1), and to establish the multiplicity of non-radial positive solutions for the case f(u) = u|u|^{p-2} when the annulus is thin. We shall first state our results for a general annulus when the right hand side of equation is of the form a(x) f(u) where the function a(x) belongs to a class of sufficiently smooth non-negative functions which enjoys certainsymmetry and monotonicity properties. This class includes the case where the function a is radial.
Adults with autism prefer person-first language in Dutch: a cross-country study
Authors: De Laet Hannah , Nijhof Annabel D. , Wiersema Jan R. 
Published: June 2023

Abstract

The correct language to refer to someone with a diagnosis of autism spectrum disorder has received a lot of attention in recent years. Studies in English-speaking countries found a main identity first-language (IFL) preference (e.g. autistic person) opposed to a person-first language preference (PFL) (e.g. person with autism) among adults with autism. However, a recent study conducted in a Dutch-speaking country (the Netherlands) reported a PFL preference (Buijsman et al., 2023). In the current study, we asked 414 Dutch-speaking adults with autism, living either in Belgium or the Netherlands, to fill in an online questionnaire about their language preference. We found that over half of the participants had a PFL preference (54%), followed by having no preference (27%). Only 14% of them had an IFL preference, and 5% proposed another term. Having more years of education was identified as a predictor for having an IFL preference when compared to a PFL preference, while being older predicted having no preference compared to a PFL preference. In conclusion, the majority of Dutch-speaking adults with autism showed a PFL preference, which is in contrast to findings from English-speaking countries, but in accord with a recent study conducted in the Netherlands (Buijsman et al., 2023). Implications of this finding for language use are discussed.
La Psicopedagogía, orientación y tutoría educativa como herramienta para la mejora de la educación y formación profesional
Authors: Rafael Hidalgo Bertha Luz , Orbegoso Rivera Viky , Lucero Rafael Víctor Antonio de Jesús 
Published: June 2023

El presente artículo nos permite valorar la importancia de la psicopedagogía, la orientación y tutoría, herramientas valiosas que constituyen una oportunidad para potenciar al máximo la educación, formación, aspectos profesionales y conseguir objetivos profesionales y de mejora competencial. La psicopedagogía contribuye a preservar, mejorar, mantener en las y los estudiantes contribuyendo con su proceso de enseñanza-aprendizaje, creando condiciones de mejora algunas de manera individual y otras grupal, también realizar de manera adecuada la evaluación, diagnóstico y la ayuda, de una forma personalizada, a aquellos estudiantes que presentan dificultades en el aprendizaje. La orientación también busca apoyar a los estudiantes en el ámbito lo académico, para que logren mejores desempeños en las actividades que desarrollan, a fin de poder prevenir y superar vivencias de fracaso y frustración que puedan alejarlos de la experiencia educativa. La tutoría ayuda a los estudiantes a desarrollar y utilizar estrategias de pensamiento, de autoaprendizaje, de administración del tiempo, de trabajo en equipo y de motivación para el estudio. Fomenta en los estudiantes y profesionales habilidades y actitudes que les permitan optimizar su propio proceso de aprendizaje se busca que los estudiantes de todos los niveles y profesionales identifiquen sus logros y dificultades en el campo académico y las relacionen con sus metas personales.
Modeling reference evapotranspiration using machine learning and remote sensing techniques for semi-arid subtropical climate of Indian Punjab
Authors: Duhan Darshana , Singh Mahesh Chand , Singh Dharmendra , Satpute Sanjay , Singh Sompal , Prasad Vishnu 
Published: June 2023

Abstract

A study was carried out to develop and evaluate the performance of different machine learning (ML) models for predicting reference evapotranspiration (ET0). The models included multiple linear regression (MLR), least square-support vector machine (LS-SVM), artificial neural networks (ANNs) and adaptive neuro-fuzzy inference system (ANFIS). The daily meteorological data for 50 years (1970–2019) were used to estimate ET0 using FAO-ET calculator. The FAO-ET calculator was compared with ML models to investigate the best-fit ML model for predicting ET. Thereafter, ET predicted by the best-fit ML model was compared with satellite (Moderate Resolution Imaging Spectroradiometer – MODIS) ET, which was finally mapped to a larger landscape (over entire Punjab and Haryana). Modeling of ET0 was best performed through LS-SVM followed by ANN2, ANN1, ANFIS10, ANFIS2, MLR and ANFIS9 models. Among developed models, coefficient of determination (R2) value varied from 0.800 to 0.998, being highest (0.998) under LS-SVM model. MODIS overestimated ET when compared with LS-SVM having R2 and root mean square error (RMSE) values of 0.73 and 3.95 mm, respectively. After applying the bias correction factor, R2 and RMSE were 0.74 and 1.19 mm, respectively. The ML and satellite-based ET estimation would be useful for timely water budgeting to manage the water scarcity problems from local to regional levels.
The Variability of Lumbar Sequential Motion Patterns: Observational Study
Authors: Caelers Inge , Boselie Toon , van Hemert Wouter , Rijkers Kim , De Bie Rob , van Santbrink Henk 
Published: June 2023

Background
Physiological motion of the lumbar spine is a topic of interest for musculoskeletal health care professionals since abnormal motion is believed to be related to lumbar complaints. Many researchers have described ranges of motion for the lumbar spine, but only few have mentioned specific motion patterns of each individual segment during flexion and extension, mostly comprising the sequence of segmental initiation in sagittal rotation. However, an adequate definition of physiological motion is still lacking. For the lower cervical spine, a consistent pattern of segmental contributions in a flexion-extension movement in young healthy individuals was described, resulting in a definition of physiological motion of the cervical spine.

Objective
This study aimed to define the lumbar spines’ physiological motion pattern by determining the sequence of segmental contribution in sagittal rotation of each vertebra during maximum flexion and extension in healthy male participants.

Methods
Cinematographic recordings were performed twice in 11 healthy male participants, aged 18-25 years, without a history of spine problems, with a 2-week interval (time point T1 and T2). Image recognition software was used to identify specific patterns in the sequence of segmental contributions per individual by plotting segmental rotation of each individual segment against the cumulative rotation of segments L1 to S1. Intraindividual variability was determined by testing T1 against T2. Intraclass correlation coefficients were tested by reevaluation of 30 intervertebral sequences by a second researcher.

Results
No consistent pattern was found when studying the graphs of the cinematographic recordings during flexion. A much more consistent pattern was found during extension, especially in the last phase. It consisted of a peak in rotation in L3L4, followed by a peak in L2L3, and finally, in L1L2. This pattern was present in 71% (15/21) of all recordings; 64% (7/11) of the participants had a consistent pattern at both time points. Sequence of segmental contribution was less consistent in the lumbar spine than the cervical spine, possibly caused by differences in facet orientation, intervertebral discs, overprojection of the pelvis, and muscle recruitment.

Conclusions
In 64% (7/11) of the recordings, a consistent motion pattern was found in the upper lumbar spine during the last phase of extension in asymptomatic young male participants. Physiological motion of the lumbar spine is a broad concept, influenced by multiple factors, which cannot be captured in a firm definition yet.

Trial Registration
ClinicalTrials.gov NCT03737227; https://clinicaltrials.gov/ct2/show/NCT03737227

International Registered Report Identifier (IRRID)
RR2-10.2196/14741
Short-Term Findings From Testing EPIO, a Digital Self-Management Program for People Living With Chronic Pain: Randomized Controlled Trial
Authors: Bostrøm Katrine , Børøsund Elin , Eide Hilde , Varsi Cecilie , Kristjansdottir Ólöf Birna , Schreurs Karlein M G , Waxenberg Lori B , Weiss Karen E , Morrison Eleshia J , Stavenes Støle Hanne , Cvancarova Småstuen Milada , Stubhaug Audun , Solberg Nes Lise 
Published: June 2023

Background
Chronic pain conditions involve numerous physical and psychological challenges, and while psychosocial self-management interventions can be of benefit for people living with chronic pain, such in-person treatment is not always accessible. Digital self-management approaches could improve this disparity, potentially bolstering outreach and providing easy, relatively low-cost access to pain self-management interventions.

Objective
This randomized controlled trial aimed to evaluate the short-term efficacy of EPIO (ie, inspired by the Greek goddess for the soothing of pain, Epione), a digital self-management intervention, for people living with chronic pain.

Methods
Patients (N=266) were randomly assigned to either the EPIO intervention (n=132) or a care-as-usual control group (n=134). Outcome measures included pain interference (Brief Pain Inventory; primary outcome measure), anxiety and depression (Hospital Anxiety and Depression Scale), self-regulatory fatigue (Self-Regulatory Fatigue 18 scale), health-related quality of life (SF-36 Short Form Health Survey), pain catastrophizing (Pain Catastrophizing Scale), and pain acceptance (Chronic Pain Acceptance Questionnaire). Linear regression models used change scores as the dependent variables.

Results
The participants were primarily female (210/259, 81.1%), with a median age of 49 (range 22-78) years and a variety of pain conditions. Analyses (n=229) after 3 months revealed no statistically significant changes for the primary outcome of pain interference (P=.84), but significant reductions in the secondary outcomes of depression (mean difference −0.90; P=.03) and self-regulatory fatigue (mean difference −2.76; P=.008) in favor of the intervention group. No other statistically significant changes were observed at 3 months (all P>.05). Participants described EPIO as useful (ie, totally agree or agree; 95/109, 87.2%) and easy to use (101/109, 92.7%), with easily understandable exercises (106/109, 97.2%).

Conclusions
Evidence-informed, user-centered digital pain self-management interventions such as EPIO may have the potential to effectively support self-management and improve psychological functioning in the form of reduced symptoms of depression and improved capacity to regulate thoughts, feelings, and behavior for people living with chronic pain.

Trial Registration
ClinicalTrials.gov NCT03705104; https://clinicaltrials.gov/ct2/show/NCT03705104
103-OR: LX9211, an Orally-Administered, Non-opioid, AAK1 Inhibitor for Painful Diabetic Peripheral Neuropathy—Results from a Randomized, Double-Blind, Placebo-Controlled, Parallel-Group, Multicenter Study
Authors: BUSUI RODICA , PATEL ANAND , SANG CHRISTINE N. , GRANOWITZ CRAIG B. , BANKS PHILLIP L. , PIERCE PHILLIP , SUN FRANKLIN W. , GOPINATHAN SUMA 
Published: June 2023

Diabetic peripheral neuropathy (DPN) is a common complication in individuals with diabetes with high morbidity and mortality. Painful DPN impacts quality of life and is very challenging to treat. The efficacy and safety of LX9211, a potent, non-opioid inhibitor of a novel target, AP2-associated protein kinase 1 (AAK1) was evaluated in a Phase 2 randomized, double-blind, placebo-controlled, parallel-group, multicenter clinical study (RELIEF-DPN 1). The baseline characteristics [mean (SD)] of 319 adults were age 62.2 (9.94) years, A1C 7.7 (1.28) %, BMI 32.07 (4.45) kg/m2, and average daily pain score (ADPS) after placebo run-in period 6.55 (1.089). Patients were randomized to either placebo, LX9211 100 mg/10 mg (100 mg Day 1, 10 mg thereafter-Low Dose arm), or LX9211 200 mg/20 mg (200 mg Day 1, 20 mg thereafter-High Dose arm) for 6 weeks followed by 5-week safety follow-up period. At Week 6, LX9211 was associated with significant improvement in Neuropathic Pain Symptom Inventory (NPSI) total score (Low Dose: -5.18, p=0.064; High Dose: -7.22, p=0.008), burning pain (Low Dose: -1.40; p&lt;0.001; High Dose: -0.89, p=0.017), pain interference on sleep (Low Dose: -0.96, p=0.005; High Dose: -1.04, p=0.002) and Patient Global Impression of Change score (Low Dose: -0.35, p=0.031; High Dose: -0.15, p=0.351) compared to placebo while achieving the primary endpoint (change from placebo in ADPS) in the low dose arm. LX9211 was safe (no treatment-related SAEs or deaths) and did not affect key cardiometabolic parameters (body weight, blood pressure, glucose control, cholesterol levels). RELIEF-DPN 1 demonstrated significant improvements in validated neuropathic pain instruments and in other patient reported outcomes suggesting that LX9211 is a promising, opioid independent new avenue to treat people with painful DPN. Confirmation of these positive results are planned in future Phase 3 trials.

Disclosure
R.Busui: Board Member; American Diabetes Association, Consultant; Averitas Pharma, Inc., Lexicon Pharmaceuticals, Inc., Nevro Corp., Novo Nordisk, Roche Diagnostics, Procter & Gamble, Research Support; Novo Nordisk, Medtronic, National Institutes of Health. A.Patel: Other Relationship; Lexicon Pharmaceuticals, Inc. C.N.Sang: Consultant; Arena Pharmaceuticals, Inc., Covance, SOLVD, Other Relationship; Kolon, Research Support; AbbVie Inc., Merck & Co., Inc., Tonix, Lexicon Pharmaceuticals, Inc., Vivozon, Helixmith. C.B.Granowitz: None. P.L.Banks: Employee; Lexicon Pharmaceuticals, Inc. P.Pierce: Employee; Lexicon Pharmaceuticals, Inc. F.W.Sun: Employee; Lexicon Pharmaceuticals, Inc. S.Gopinathan: Employee; Lexicon Pharmaceuticals, Inc.

Funding
Lexicon Pharmaceuticals, Inc.
1044-P: Advancing Quality Improvement Culture among 27 Pediatric and Adult Diabetes Centers
Authors: MUNGMODE ANN , ODUGBESAN ORI , ELISHA VICTOR , STEENKAMP DEVIN W. , KAMBOJ MANMOHAN K. , DESALVO DANIEL , SONABEND RONA Y. , NEYMAN ANNA , MATHIAS PRIYANKA M. , CHAO LILY , ILKOWITZ JENIECE , EBEKOZIEN OSAGIE , COLLABORATIVE T1DX-QI 
Published: June 2023

Background: The T1D Exchange Quality Improvement Collaborative (T1DX-QI) is a learning health system of over fifty endocrinology centers working to improve diabetes outcomes through Quality Improvement (QI). Centers join T1DX-QI with varying levels of QI culture and are assigned a QI Coach to support practice transformation. This study summarizes the results of 27 endocrinology centers involved in the T1DX-QI for over 12 months.
Method: Centers completed a 20-question self-assessment survey to measure QI culture over time in four evidence-based areas: QI Team Structure (team roles); QI Foundation (data collection and training); QI Capacity (use of QI tools); and QI Success (scaling up small tests). Data was collected in 2021 and 2022 for the same centers. The overall difference in QI Capacity was calculated using a Chi-Square test.
Results: Overall, QI Culture increased from 67% to 75% from 2021 to 2022 (p-value = 0.005). Figure 1 demonstrates that improvements were seen in three of the four evidence-based areas for both pediatric and adult centers in this time.
Conclusion: Even with as little as one year of involvement with the T1DX-QI, centers experience an improvement in their QI Capacity.

Disclosure
A.Mungmode: None. L.Chao: None. J.Ilkowitz: None. O.Ebekozien: Advisory Panel; Medtronic, Research Support; Eli Lilly and Company, Dexcom, Inc. T1dx-qi collaborative: n/a. O.Odugbesan: None. V.Elisha: None. D.W.Steenkamp: Research Support; Abbott Diabetes, Novo Nordisk. M.K.Kamboj: None. D.Desalvo: Consultant; Dexcom, Inc., Research Support; Insulet Corporation. R.Y.Sonabend: None. A.Neyman: None. P.M.Mathias: None.

Funding
The Leona M. and Harry B. Helmsley Charitable Trust
1055-P: Clinical Decision Support Systems (CDSSs) in Diabetes Management—A Scoping Review
Authors: HUANG SHAN , LIANG YUZHEN , LI JIARUI , JUN LI XUE 
Published: June 2023

This scoping review aimed to summarize the application scenarios, effectiveness and safety of CDSS in diabetes management. PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang, VIP, and conference databases were searched from 1985 to 2022. There were 11,145 studies identified, and 72 articles and 8 abstracts were included, involving 137,710 subjects. Most CDSSs (77%) were knowledge-based, while the non-knowledge-based systems have been increasing in recent years. CDSS was mainly utilized in treatment recommendations (Table 1). Most CDSSs significantly improved outcomes (Table 2) without increase of hypoglycemia risk. The results demonstrated the value of CDSS as a reliable assistant in diabetes management especially in primary care with limited resources, and advanced software is needed to improve CDSS functionalities.

Disclosure
S.Huang: None. Y.Liang: None. J.Li: None. X.Li: None.

Funding
Beijing Medical Award Foundation (2019-1585)