bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating nanocatalyst-embedding laser-induced carbon nanofibers for non-enzymatic electrochemical sensing of hydrogen peroxide
Authors: Bruckschlegel Christoph , Schlosser Marc , Wongkaew Nongnoot 
Published: March 2023

AbstractIn this present study, we explored the catalytic behaviors of the in situ generated metal nanoparticles, i.e., Pt/Ni, embedded in laser-induced carbon nanofibers (LCNFs) and their potential for H2O2 detection under physiological conditions. Furthermore, we demonstrate current limitations of laser-generated nanocatalyst embedded within LCNFs as electrochemical detectors and possible strategies to overcome the issues. Cyclic voltammetry revealed the distinctive electrocatalytic behaviors of carbon nanofibers embedding Pt and Ni in various ratios. With chronoamperometry at  +0.5 V, it was found that modulation of Pt and Ni content affected only current related to H2O2 but not other interfering electroactive substances, i.e., ascorbic acid (AA), uric acid (UA), dopamine (DA), and glucose. This implies that the interferences react to the carbon nanofibers regardless of the presence of metal nanocatalysts. Carbon nanofibers loaded only with Pt and without Ni performed best in H2O2 detection in phosphate-buffered solution with a limit of detection (LOD) of 1.4 µM, a limit of quantification (LOQ) of 5.7 µM, a linear range from 5 to 500 µM, and a sensitivity of 15 µA mM−1 cm−2. By increasing Pt loading, the interfering signals from UA and DA could be minimized. Furthermore, we found that modification of electrodes with nylon improves the recovery of H2O2 spiked in diluted and undiluted human serum. The study is paving the way for the efficient utilization of laser-generated nanocatalyst-embedding carbon nanomaterials for non-enzymatic sensors, which ultimately will lead to inexpensive point-of-need devices with favorable analytical performance.
Graphical abstract
Dirac Operators for Matrix Algebras Converging to Coadjoint Orbits
Authors: Rieffel Marc A. 
Published: March 2023

AbstractIn the high-energy physics literature one finds statements such as “matrix algebras converge to the sphere”. Earlier I provided a general precise setting for understanding such statements, in which the matrix algebras are viewed as quantum metric spaces, and convergence is with respect to a quantum Gromov–Hausdorff-type distance. But physicists want even more to treat structures on spheres (and other spaces), such as vector bundles, Yang–Mills functionals, Dirac operators, etc., and they want to approximate these by corresponding structures on matrix algebras. In the present paper we provide a somewhat unified construction of Dirac operators on coadjoint orbits and on the matrix algebras that converge to them. This enables us to prove our main theorem, whose content is that, for the quantum metric-space structures determined by the Dirac operators that we construct, the matrix algebras do indeed converge to the coadjoint orbits, for a quite strong version of quantum Gromov–Hausdorff distance.
The influence of abiotic and biotic conditions on lifecycle stages is critical for estuarine seagrass resilience
Authors: Webster Chanelle , Lavery Paul S. , O’Dea Caitlyn , Sánchez Alarcón Marta , Salgado Kent Chandra , McMahon Kathryn 
Published: March 2023

AbstractAbiotic and biotic factors influence seagrass resilience, but the strength and relative importance of the effects are rarely assessed over the complete lifecycle. This study examined the effects of abiotic (salinity, temperature, water depth) and biotic (grazing by black swans) factors on Ruppia spp. over the complete lifecycle. Structures were set up in two estuaries ( – 33.637020, 115.412608) that prevented and allowed natural swan grazing of the seagrasses in May 2019, before the start of the growing season. The density of life stage(s) was measured from June 2019 when germination commenced through to January 2020 when most of the seagrass senesced. Our results showed that swans impacted some but not all life stages. Seedling densities were significantly higher in the plots that allowed natural grazing compared to the exclusion plots (e.g. 697 versus 311 seedlings per m-2), revealing an apparent benefit of swans. Swans removed ≤ 10% of seagrass vegetation but a dormant seedbank was present and new propagules were also observed. We conclude that grazing by swans provides some benefit to seagrass resilience by enhancing seedling recruitment. We further investigated the drivers of the different lifecycle stages using general additive mixed models. Higher and more variable salinity led to increased seed germination whilst temperature explained variation in seedling density and adult plant abundance. Bet-hedging strategies of R. polycarpa were revealed by our lifecycle assessment including the presence of a dormant seedbank, germinated seeds and seedlings over the 8-month study period over variable conditions (salinity 2–42 ppt; temperatures 11–28 °C). These strategies may be key determinants of resilience to emerging salinity and temperature regimes from a changing climate.
Post-closure Cost Efficiency in Public Versus Private Landfills: The Case of Emilia-Romagna (Italy)
Authors: Tallaki Mouhcine , Bracci Enrico , Ievoli Riccardo 
Published: March 2023

AbstractWaste management systems have developed in recent years toward the adoption of sustainable management principles and practices, such as circular economy, zero waste, resource efficiency, waste avoidance, re-use, and recycling. Nevertheless, landfills continue to be used for waste disposal despite their risks related to contamination and effects on urban development. Most research on landfills focuses on their operational and technical aspects, while the performance and cost efficiency in managing landfills is less commonly studied, especially their post-closure management. However, improving efficiency is very relevant in the context of scarce public sector resources. This paper, therefore, analyzes the efficiency of post-closure management of landfills. Drawing on agency and stewardship theories, we focus on the difference in efficiency between public and private management of post-closure landfills. We use a linear mixed regression model to analyze data from 2015 to 2018 relating to 54 landfills (79% of which are privately managed) in the Emilia-Romagna region of Italy. The results show that public management is more efficient than private management. Results contribute to defining drivers of cost and confirming a disparity in the performance of private and public management. Our results cast doubt on the assumption, which is prevalent in new public management theory, that private operators are more efficient than public ones. We conclude by highlighting that to reach efficiency, it is better to increase the effectiveness of regulation in terms of value for money, without pre-determined preferences for the type of management.
Practical aspects of designing background-oriented schlieren (BOS) experiments for vortex measurements
Authors: Schwarz Clemens , Braukmann Johannes N. 
Published: March 2023

AbstractSetup-related aspects of background-oriented schlieren (BOS) experiments are discussed focusing on a sensitivity parameterS, which represents the relation between light deflection and resulting BOS signal, and the geometric blur. An analytic expression for the geometric blur by means of the circle of confusion (CoC) was derived which shows a proportional relation to the sensitivity factorS. The theoretical findings were validated in a reference experiment using generic distortions in glass plates. It was found that the filtering effect of the blur decreases the maximum background shift and its influence can be expressed with a blur loss factorB, which depends on the size of the CoC in relation to the investigated object. Multiplying the setup sensitivitySwith the blur lossBresults in the effective sensitivity$$S _{{\rm eff}}$$Seffthat determines the maximum achievable BOS signal of a schlieren object. For the investigated reference objects, the maximum effective sensitivity$$S _{{\rm eff}}$$Seffwas found to occur at CoC sizes in the object domain from 2.5 to 3.8 times the extent of the investigated objects. A step-by-step method is proposed for designing BOS experiments to obtain a maximum signal strength. The design parameters are further discussed specifically in regard to rotor tip vortex visualization, for which a variety of previously reported experiments are compared. A simple prediction method for the BOS signal of blade tip vortices is proposed and validated with experimental data from a rotor test stand. The application of the method to rotor systems of different size shows the requirement for increasingly higher sensitivity values for visualizing vortices of small-scale rotors.Graphical abstract
Vortex ring state of a shrouded rotor: an experimental survey
Authors: Pickles David J. , Zagaglia Daniele , Busse Angela , Green Richard B. 
Published: March 2023

Abstract
The vortex ring state (VRS) is a flow condition typical of rotors operating in axial descent flight which may lead to large unpredictable thrust oscillations and possibly the loss of control of the rotorcraft. Despite the dangers associated with this flight condition, there is a distinct lack of detailed experimental data related to shrouded rotors operating in axial descent, which is of relevance owing to the number of novel unmanned aerial vehicles that incorporate this technology. This manuscript presents an experimental investigation designed to assess if and how the presence of the shroud affects the development of the vortex ring state. To this end, laser Doppler anemometry and particle image velocimetry were used to investigate the flow for a range of descent velocities and results were compared with those obtained without the shroud. Time-averaged data were used to assess the general structures of the flow fields, whilst statistical analysis of the velocity fluctuations and modal analysis of the velocity field using proper orthogonal decomposition highlighted the unsteady features of the flow. The investigation showed that shrouded rotors enter the VRS similarly to their isolated counterparts, and the presence of the shroud may be responsible for a slight delay of its onset.

Graphical abstract
Glioneuronal tumor with ATRX alteration, kinase fusion and anaplastic features (GTAKA): a molecularly distinct brain tumor type with recurrent NTRK gene fusions
Authors: Bogumil Henri , Sill Martin , Schrimpf Daniel , Ismer Britta , Blume Christina , Rahmanzade Ramin , Hinz Felix , Cherkezov Asan , Banan Rouzbeh , Friedel Dennis , Reuss David E. , Selt Florian , Ecker Jonas , Milde Till , Pajtler Kristian W. , Schittenhelm Jens , Hench Jürgen , Frank Stephan , Boldt Henning B. , Kristensen Bjarne Winther , Scheie David , Melchior Linea C. , Olesen Viola , Sehested Astrid , Boué Daniel R. , Abdullaev Zied , Satgunaseelan Laveniya , Kurth Ina , Seidlitz Annekatrin , White Christine L. , Ng Ho-Keung , Shi Zhi-Feng , Haberler Christine , Deckert Martina , Timmer Marco , Goldbrunner Roland , Tauziède-Espariat Arnault , Varlet Pascale , Brandner Sebastian , Alexandrescu Sanda , Snuderl Matija , Aldape Kenneth , Korshunov Andrey , Witt Olaf , Herold-Mende Christel , Unterberg Andreas , Wick Wolfgang , Pfister Stefan M. , von Deimling Andreas , Jones David T. W. , Sahm Felix , Sievers Philipp 
Published: March 2023

AbstractGlioneuronal tumors are a heterogenous group of CNS neoplasms that can be challenging to accurately diagnose. Molecular methods are highly useful in classifying these tumors—distinguishing precise classes from their histological mimics and identifying previously unrecognized types of tumors. Using an unsupervised visualization approach of DNA methylation data, we identified a novel group of tumors (n = 20) that formed a cluster separate from all established CNS tumor types. Molecular analyses revealed ATRX alterations (in 16/16 cases by DNA sequencing and/or immunohistochemistry) as well as potentially targetable gene fusions involving receptor tyrosine-kinases (RTK; mostly NTRK1-3) in all of these tumors (16/16; 100%). In addition, copy number profiling showed homozygous deletions of CDKN2A/B in 55% of cases. Histological and immunohistochemical investigations revealed glioneuronal tumors with isomorphic, round and often condensed nuclei, perinuclear clearing, high mitotic activity and microvascular proliferation. Tumors were mainly located supratentorially (84%) and occurred in patients with a median age of 19 years. Survival data were limited (n = 18) but point towards a more aggressive biology as compared to other glioneuronal tumors (median progression-free survival 12.5 months). Given their molecular characteristics in addition to anaplastic features, we suggest the term glioneuronal tumor with ATRX alteration, kinase fusion and anaplastic features (GTAKA) to describe these tumors. In summary, our findings highlight a novel type of glioneuronal tumor driven by different RTK fusions accompanied by recurrent alterations in ATRX and homozygous deletions of CDKN2A/B. Targeted approaches such as NTRK inhibition might represent a therapeutic option for patients suffering from these tumors.
A prospective study of cellular immune response to booster COVID-19 vaccination in multiple sclerosis patients treated with a broad spectrum of disease-modifying therapies
Authors: Torres Pascual , Sancho-Saldaña Agustín , Gil Sánchez Anna , Peralta Silvia , Solana Maria José , Bakkioui Sofian , González-Mingot Cristina , Quibus Laura , Ruiz-Fernández Emilio , San Pedro-Murillo Eduardo , Brieva Luis 
Published: March 2023

Abstract
Background
Most people with Multiple Sclerosis (pwMS) are subjected to immunomodulatory disease-modifying treatments (DMTs). As a result, immune responses to COVID-19 vaccinations could be compromised. There are few data on cellular immune responses to the use of COVID-19 vaccine boosters in pwMS under a broad spectrum of DMTs.

Methods
In this prospective study, we analysed cellular immune responses to SARS-CoV-2 mRNA booster vaccinations in 159 pwMS with DMT, including: ocrelizumab, rituximab, fingolimod, alemtuzumab, dimethyl fumarate, glatiramer acetate, teriflunomide, natalizumab and cladribine.

Results
DMTs, and particularly fingolimod, interact with cellular responses to COVID-19 vaccination. One booster dose does not increase cellular immunity any more than two doses, except in the cases of natalizumab and cladribine. SARS-CoV-2 infection combined with two doses of vaccine resulted in a greater cellular immune response, but this was not observed after supplementary booster jabs. Ocrelizumab-treated pwMS who had previously received fingolimod did not develop cellular immunity, even after receiving a booster. The time after MS diagnosis and disability status negatively correlated with cellular immunity in ocrelizumab-treated pwMS in a booster dose cohort.

Conclusions
After two doses of SARS-CoV-2 vaccination, a high response yield was achieved, except in patients who had received fingolimod. The effects of fingolimod on cellular immunity persisted for more than 2 years after a change to ocrelizumab (which, in contrast, conserved cellular immunity). Our results confirmed the need to find alternative protective measures for fingolimod-treated people and to consider the possible failure to provide protection against SARS-CoV-2 when switching from fingolimod to ocrelizumab.
Predictors of return to work for people on sick leave with depression, anxiety and stress: secondary analysis from a randomized controlled trial
Authors: Bjørkedal Siv-Therese , Fisker Jonas , Hellström Lone Christina , Hoff Andreas , Poulsen Rie Mandrup , Hjorthøj Carsten , Bojesen Anders Bo , Rosenberg Nicole Gremaud , Eplov Lene Falgaard 
Published: March 2023

Abstract
Purpose
Knowledge about predictors of return to work (RTW) in people on sick leave with common mental disorders (CMDs) may inform the development of effective vocational rehabilitation interventions for this target group. In this study, we investigated predictors of RTW at 6 and 12 months in people on sick leave with depression, anxiety disorders or stress-related disorders.

Methods
We have performed a secondary analysis, utilizing data from two RCTs that evaluated the efficacy of an integrated health care and vocational rehabilitation intervention. Data were obtained from mental health assessments, questionnaires and registers. Using Cox regression analysis, the relationship between baseline variables and RTW was analysed at 6 and 12 months after randomization within the group of CMD as a whole and within the subgroups of depression, anxiety and stress-related disorders.

Results
Symptom burden and employment status at baseline predicted RTW in the CMD group (n = 1245) and in the three diagnostic subgroups at both time points. RTW self-efficacy predicted RTW in the depression group but not in the anxiety or stress subgroups.

Conclusion
Many predictors of RTW were similar over time and, to some extent, across the CMD subgroups. Findings highlight the need not only to take health-related and psychological factors into account when developing vocational rehabilitation interventions but also to consider workplace strategies and options for support.
The complete dorsal structure is formed from only the blastocoel roof of Xenopus blastula: insight into the gastrulation movement evolutionarily conserved among chordates
Authors: Sato Yuki , Narasaki Izumi , Kunimoto Takuya , Moriyama Yuki , Hashimoto Chikara 
Published: March 2023

AbstractGastrulation is a critical event whose molecular mechanisms are thought to be conserved among vertebrates. However, the morphological movement during gastrulation appears to be divergent across species, making it difficult to discuss the evolution of the process. Previously, we proposed a novel amphibian gastrulation model, the “subduction and zippering (S&Z) model”. In this model, the organizer and the prospective neuroectoderm are originally localized in the blastula’s blastocoel roof, and these embryonic regions move downward to make physical contact of their inner surfaces with each other at the dorsal marginal zone. The developmental stage when contact between the head organizer and the anterior-most neuroectoderm is established is called “anterior contact establishment (ACE).” After ACE, the A-P body axis elongates posteriorly. According to this model, the body axis is derived from limited regions of the dorsal marginal zone at ACE. To investigate this possibility, we conducted stepwise tissue deletions using Xenopus laevis embryos and revealed that the dorsal one-third of the marginal zone had the ability to form the complete dorsal structure by itself. Furthermore, a blastocoel roof explant of the blastula, which should contain the organizer and the prospective neuroectoderm in the S&Z model, autonomously underwent gastrulation and formed the complete dorsal structure. Collectively, these results are consistent with the S&Z gastrulation model and identify the embryonic region sufficient for construction of the complete dorsal structure. Finally, by comparing amphibian gastrulation to gastrulation of protochordates and amniotes, we discuss the gastrulation movement evolutionarily conserved among chordates.
Molecular characterization of haemosporidian and haemogregarine diversity in southwestern Iberian amphibians and reptiles
Authors: Parejo-Pulido Daniel , Mora-Rubio Carlos , Marzal Alfonso , Magallanes Sergio 
Published: March 2023

AbstractThe knowledge of the diversity and geographic distribution of parasite species is the first step towards understanding processes of global epidemiology and species conservation. Despite recent increases in research on haemosporidian and haemogregarine parasites of reptiles and amphibians, we still know little about their diversity and parasite-host interactions, especially in the Iberian Peninsula, where a few studies have been conducted. In this study, the haemosporidian and haemogregarine diversity and phylogenetic relationships of the parasites in southwestern Iberian amphibians and reptiles were assessed using PCR approaches on blood samples of 145 individuals from five amphibian and 13 reptile species. The amphibians did not present any of both groups of parasites studied. Regarding reptiles, five Hepatozoon, one Haemogregarina, and one Haemocystidum haplotypes were found infecting four different species, revealing new host records for these parasites. Among them, we found one new Haemocystidium haplotype and three new and a previously reported Hepatozoon haplotype from a north African snake. The latter finding suggests that some Hepatozoon parasites may not be host-specific and have large geographic ranges even crossing geographical barriers. These results increased the knowledge about the geographic distribution and the number of known host species of some reptile apicomplexan parasites, highlighting the great unexplored diversity of them in this region.
K[(CuII,MnII,MnIII)2(TeO3)3]∙2H2O, the first zemannite-type structure based on a Jahn-Teller-distorted framework
Authors: Eder Felix , Miletich Ronald , Weil Matthias 
Published: March 2023

AbstractSynthetic single crystals of K[(CuII,MnII,MnIII)2(TeO3)3]·2H2O were obtained from an overconcentrated alkaline aqueous solution in the system K2O-MnO-CuO-TeO2 under hydrothermal conditions at T ≤ 220 °C. Subeuhedral single crystals have been investigated by means of single-crystal X-ray diffraction. The crystal structure of this new zemannite-type representative adopts a monoclinic twofold superstructure. The doubling of the unit-cell volume is accompanied by a hexagonal-to-monoclinic symmetry reduction, resulting in threefold twinning with individual crystal domains following the space group symmetry P21. Refinements of site-occupation factors and the evaluation of bond valences suggest a distribution of di- and trivalent cations at the octahedrally coordinated M sites with a ratio (CuII + MnII):MnIII approximating 1:1. Based on arguments about the cation sizes and the individual bond valence sums, a distribution of CuII1–xMnIIIx and MnIIx MnIII1–x at each two of the four M sites can be assumed with x between ~ 0.14 and ~ 0.50. The K+ cations and H2O molecules inside the channels are located off the central channel axis. In contrast to most other known zemannite-type phases, the extra-framework atoms show full occupancies and are not disordered. The distribution of the channel contents supports the anisotropic deformation of the surrounding framework, which follows the local symmetry reduction as required for the Jahn-Teller distortion of the octahedrally coordinated M sites within the framework. The arrangement of the deformed channels can be understood as the origin of the existing superstructure.