bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined Application of Salinomycin and ATRA Induces Apoptosis and Differentiation
of Acute Myeloid Leukemia Cells by Inhibiting WNT/β-Catenin Pathway
Authors: Xi Hui-Min , Lu Hao , Weng Xiang-Qin , Sheng Yan , Wu Jing , Li Lu , Cai Xun 
Published: January 2023

Background and objective:
All-trans retinoic acid (ATRA) is only effective in acute promyelocytic leukemia (APL), but not in other subtype of acute myeloid leukemia (AML). Salinomycin targets tumor cells rather than non-tumorigenic cells, and WNT/β-catenin pathway inhibition is one of the mechanisms of its anti-tumor activity. There is a crosstalk between RA and WNT/β-catenin pathway. Here, we investigate the effect of the combination of salinomycin and ATRA (S+RA) in non-APL AML cells.

Methods:
Apoptosis was evaluated by cell viability and Annexin-V assay. Cell differentiation was analyzed by CD11c expression and morphology. To explore the underlying mechanisms, Western blot analysis and mitochondrial transmembrane potentials (m) were used.

Results & Discussion:
S+RA induced differentiation and apoptosis in AML cell lines and AML primary cells. S+RA inhibited the β-catenin signal pathway as determined by the decreased protein levels of β-catenin, the low-density lipoprotein receptor-related proteins 6 (LRP6), and its downstream proteins such as survivin, c-Myc, caspase-3/7, cdc25A and cyclinD1 and reduced phosphorylation level of GSK3β S9. S+RA also increased the protein levels of CCAAT/enhancer-binding proteins (C/EBPs) and PU.1 and collapsed m. The above molecular and cellular changes induced by S+RA were inhibited by β-catenin specific activator and promoted by β-catenin specific inhibitor.

Conclusion:
S+RA induced differentiation by β-catenin-inhibition-mediated up-regulation of C/EBPs and PU.1 and suppression of c-Myc. S+RA triggered apoptosis through β-catenin-inhibition-regulated m collapse and caspase-3/7 activation. Taken together, our findings may provide novel therapeutic strategies for AML patients by targeting the WNT/β-catenin pathway.
Inflammatory Indices during and after a Randomized Controlled Trial on Exercise in Old Adults: Could Moderate-intensity Exercise be Safe Enough? (Active Elderly and Health – Clinicaltrials.Gov, NCT03858114)
Authors: Scano Alessandra , Cossu Giulia , Kalcev Goce , Pintus Elisa , Pau Massimiliano , Penna Maria Petronilla , Atzori Laura , Orrù Germano , Romano Ferdinando , Aviles Gonzalez Cesar Ivan , Carta Mauro Giovanni 
Published: January 2023

Background:
Aging is characterized by a progressive increase in chronic diseases. According to WHO’s guidance, there is a need to develop strategies to monitor, maintain and rehabilitate the health of older people. The present research aims to elucidate whether or not a moderate physical activity intervention in older adults can cause an increase in inflammatory biomarker indices such as C-reactive Protein (CRP) and erythrocyte sedimentation rate (ESR). It is a valuable tool for promoting health that can be considered tolerable in the over-65 population.

Methods:
120 males and females over 65 years of age participated in a randomized controlled trial (RCT NCT03858114) in two groups of 60 individuals of similar size and underwent moderate physical activity or cultural and recreational activities. The exclusion criteria were being younger than 65 years old, being unable to participate in physical activity for medical reasons, and a Body Mass Index of greater than 35. Blood samples from participants were collected for the pre-treatment period (t0), at the end of the study of 12 weeks (t1), and 20 weeks after the end of the study (t2) for measurement of inflammatory indices CRP and ESR.

Results:
The commonly routine tests for inflammatory reactions (ERS and CPR) showed no change at the end of an RCT on mild-to-moderate exercise.

Conclusion:
The results on inflammatory indices confirm the safety of this type of intervention and encourage its long-term use and testing.
Miłość do Boga w wybranych tekstach staropolskich dla licealistów. Refleksje na marginesie podręczników do kształcenia kulturowo-literackiego dla szkół średnich
Authors: Stachoń Maria 
Published: January 2023

Problematyka realizacji egzystencji ludzkiej w sferze sacrum zajmuje szczególne miejsce w dorobku literackim wieków dawnych. Celem artykułu jest budowanie namysłu wokół obrazu miłości do Boga utrwalonego w poezji dawnej, omawianej na lekcjach języka polskiego w szkole średniej. Ważna w tym aspekcie jest refleksja nad rolą i wartością obcowania z sacrum obecnym w kształceniu literackim i życiu codziennym współczesnych licealistów. W związku z tym na podstawie analizy propozycji rozwiązań metodycznych zawartych w obudowie dydaktycznej wybranych wierszy staropolskich określono kierunki projektowania refleksji o miłości poprzez analizę i interpretację wybranych wierszy oraz zaproponowanych działań lekcyjnych, mogących wspierać budowanie i pogłębianie miłości współczesnych nastolatków do sacrum, którym może być m.in. Bóg, drugi człowiek, ojczyzna.
GAMBARAN PENGGUNAAN PENGIKAT FOSFAT PADA PASIEN PENYAKIT GINJAL KRONIK DENGAN HEMODIALISIS
Authors: Sholihah Inayatush , Santoso Joko , Rahardjoputro Rolando 
Published: January 2023

<p>Pasien hemodialisis membutuhkan terapi dengan pengikat fosfat untuk menurunkan morbiditas dan mortalitas yang berkaitan dengan tingginya kadar fosfat dalam darah. Pengikat fosfat terdiri dari agen berbasis kalsium dan agen bebas kalsium. Penelitian sebelumnya melaporkan adanya efek samping hiperkalsemia pada penggunaan pengikat fosfat berbasis kalsium dalam jangka panjang dan efek samping pada sistem gastrointestinal pada penggunaan sevelamer dan lanthanum karbonat. Penelitian ini bertujuan untuk mengetahui pola penggunaan pengikat fosfat pada pasien penyakit ginjal kronik yang menjalani hemodialisis dan analisis kesesuaian dosis berdasarkan formularium. Pengambilan data dilakukan<em> </em>secara prospektif. Subyek penelitian adalah pasien penyakit ginjal kronik yang menjalani hemodialisis di sebuah rumah sakit akademik di kabupaten Sleman, D.I Yogyakarta. Hasil penelitian dianalisis secara deskriptif. Dari 79 pasien yang memenuhi kriteria inklusi, sebanyak 49 pasien (62,03%) menggunakan kalsium karbonat, 20 pasien (25,32%) menggunakan sevelamer, 8 pasien (10,13%) menggunakan kalsium asetat, dan 2 pasien (2,53%) menggunakan lanthanum karbonat. Terdapat 3 pasien yang menerima dosis kurang dari standar dosis sevelamer yang direkomendasikan. Sebanyak 1 pasien dengan kadar fosfat 8,30 mg/dl menerima dosis 3x800 mg, sedangkan dosis standar yang direkomendasikan adalah 1200-1600 mg 3x sehari. Sebanyak 2 pasien dengan kadar fosfat 9,50 dan 9,30 mg/dl menerima dosis 3x800 mg, sedangkan dosis standar yang direkomendasikan adalah 1600 mg 3x sehari.</p>
SYNTHESIS OF MOLECULAR IMPRINTED POLYMER SALBUTAMOL USING METHACRYLIC ACID MONOMER AND TRIMETHYL PROPANE TRIMETHACRYLATE (TRIM) AS A CROSS-LINKER THROUGH SUSPENSION POLYMERIZATION
Authors: SUSANTI IKE , SAFITRI NISA , PRATIWI RIMADANI , HASANAH ALIYA NUR 
Published: January 2023

Objective: This study aims to determine the analytical performances and characteristics of MIP salbutamol made with methacrylic acid (MAA) monomer and trimethylpropane trimethacrylate (TRIM) cross-linker through suspension polymerization.
Methods: The MIP salbutamol was synthesized using suspension polymerization. The analytical performances of MIP, such as the adsorption ability, adsorption capacity and selectivity, were evaluated by Spectrophotometer UV-Vis. The physical characterization of MIP and NIP were evaluated using FTIR, TEM-EDS, Brunauer-Emmett-Teller (BET) method and Barret-Joyner-Halenda (BJH) method.
Results: Molecular Imprinted Polymer (MIP) showed better analytical performance than Non-Imprinted Polymer (NIP), the adsorption ability of MIP and NIP reached about 90.43% and 53.92%, respectively. The MIP was selective for salbutamol when compared to terbutaline and salmeterol xinafoate with an imprinting factor (IF) of 1.2841. The MIP has spherical shape particles with diameters in the range of 10-100 µm with a surface area of 185.546 m2/g, pore volume of 0.257 cm3/g, and pore size of 16.599 Å.
Conclusion: The Based on these results, MIP salbutamol, has the potential to be developed as a method for the preparation of salbutamol analysis from biological samples.
Β-LACTAMASES INHIBITOR-PRODUCING SOIL BACTERIA FOR AMPICILLIN-RESISTANT UROPATHOGENIC ESCHERICHIA COLI ISOLATE
Authors: KUSUMA SRI AGUNG FITRI , YURINA VALENTINA , RETNONINGRUM DEBBIE S. , HILMI INDAH LAILY , RAHAYU SUSI AFRIANTI , ANGGRAINI YUNI NOER 
Published: January 2023

Objective: The goals of this investigation were to identify the species of the producers and ascertain the dose-dependent effect of extracellular products of Indonesian bacteria that generate β-lactamases inhibitors.
Methods: An agar diffusion technique for the lactamase inhibitor activity assay was performed. Observation of bacteria using phenotypic analysis was performed by observing colony color and cell shape morphology, biochemical assays and a series of carbohydrate fermentation tests. Bacterial identification was performed by comparing the nucleotide sequence of the 16S rDNA gene of target bacteria with available nucleotide sequences in Gene Library (NCBI). Combining data from phenotypic and genotypic analyses allowed for the identification of the producers.
Results: According to our findings, none of the bacteria's extracellular products, which contain β-lactamase inhibitors in a range of concentrations, showed a discernible impact on the values of the inhibition zone. The producers are Aeromonas popoffii, Alcaligenes faecalis, Streptomyces brasiliensis, Staphylococcus equorum, Pseudomonas putida, Pseudomonas fluorescens, Salmonella typhi, Enterobacter hormaechei, Serratia marcescens and Enterobacter sp. The highest potency of β-lactamase inhibitor was provided by the extracellular product of VR3 isolate bacteria which was identified as Serratia marcescens.
Conclusion: In conclusion, this study clearly showed that our isolated bacteria have the potential to be further investigated in order to maximize the recovery of β-lactamase inhibitor compounds.
SCREENING OF ASHITABA (ANGELICA KEISKEI K.) COMPOUNDS AS POTENTIAL MYCOBACTERIUM TUBERCULOSIS KASA INHIBITORS
Authors: ASNAWI AIYI , FEBRINA ELLIN , ALIGITA WIDHYA , KURNIA DEWI , AMAN LA ODE , YULIANTINI ANNE 
Published: January 2023

Objective: Tuberculosis (TB) is a major global issue, mainly owing to the emergence of antibiotic-resistant strains of the disease's causative agent, Mycobacterium tuberculosis. The current standard of treatment for tuberculosis entails a prolonged course of antibiotics with toxic side effects and is accompanied by low patient compliance. Therefore, developing and discovering TB medications is critical to obtaining TB drugs that are more effective and sensitive to Mycobacterium tuberculosis. Ashitaba (Angelica keiskei K.) has reported that Ashitaba extract and chalcone have anti-TB properties, but the responsible compound has not been reported yet. This study aimed to identify the profile metabolites present in Ashitaba and their interaction with Mycobacterium tuberculosis KasA.
Methods: To suggest these, we used molecular docking and molecular dynamic to predict the interactions of 40 selected compounds from the Ashitaba against Mycobacterium tuberculosis KasA (PDB ID 2WGE).
Results: The results of molecular docking identified the top two compounds as xanthoangelol I (XAI) and (2E)-1-[4-hydroxy-2-(2-hydroxy-2-propanyl)-2,3-dihydro-1-benzofuran-7-yl]-3-(4-hydroxyphenyl)-2-propen-1-one (4HH), with bond free energies of-12.03 and-11.87 kcal/mol, respectively. Based on the results of molecular dynamics simulations, the XAI was stronger than 4HH in stabilizing complexes with 2WGE with total energy (ΔGbind, MMGBSA) of-54.8512 and-37.8836 kcal/mol, respectively.
Conclusion: It can be concluded that xanthoangelol I (XAI) have the most potent inhibitor of Mycobacterium tuberculosis KasA.
PREDICTION OF THE EFFECT OF SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE CYP2C9 ON WARFARIN METABOLISM BY IN SILICO STUDY
Authors: PUTRIANA NORISCA ALIZA , RUSDIANA TAOFIK , ROSTINAWATI TINA , AKBAR MOHAMMAD RIZKI , MEGANTARA SANDRA , HIDAYANTI SABRANAH 
Published: January 2023

Objective: This study aimed to predict the effect of SNPs CYP2C9 s on the metabolic activity of S-warfarin in the body.
Methods: Molecular modeling was performed to obtain SNPs CYP2C9 and molecular docking was performed to predict the effect of SNPs CYP2C9 on the metabolic activity of S-warfarin.
Results: The results showed that wild-type CYP2C9 had the strongest binding affinity (∆G: -9.76 kcal/mol), indicating that wild-type CYP2C9 had the best metabolic activity compared to SNPs CYP2C9. There was a decrease in hydrogen bond formation in SNPs CYP2C9 and an increase in the distance between C7 S-warfarin and Fe-Heme in CYP2C9 SNPs when compared to wild-type CYP2C9
Conclusion: The decrease in binding affinity, decrease in hydrogen bond formation, and an increase in the distance between C7 S-warfarin and Fe-Heme on SNPs CYP2C9 indicated that SNPs CYP2C9 had decreased metabolic activity against S-warfarin which led to an increased risk of bleeding.
PATTERNS OF METHAMPHETAMINE USE AND ITS ASSOCIATIONS WITH PSYCHIATRIC SYMPTOMS IN PATIENTS UPON ADMISSION AT THE NATIONAL REHABILITATION CENTER LIDO BOGOR, INDONESIA
Authors: ROHMANIKA  , AROZAL WAWAIMULI , LOUISA MELVA , GAYATRI ANGGI , KEKALIH ARIA , MARZUKI HY JEFMAN EFENDI , LESTARI ERNIAWATI , UTAMI DIAH SETIA 
Published: January 2023

Objective: Methamphetamine (MA) is a substance that is heavily abused worldwide. This present study aimed to investigate the association between the patterns of methamphetamine use and psychiatric symptoms in patients upon admission at The National Rehabilitation Center, Lido Bogor.
Methods: This was a cross-sectional study of MA abusers during the period of January 2016–December 2018 at The National Rehabilitation Center, Lido, Bogor. This study was done by assessing medical records. The inclusion criteria were MA abusers at the age of 18 y and above. Assessment of psychiatric symptoms was evaluated using Addiction Severity Index (ASI). Microsoft Excel and SPSS version 22.0 were used to process the data.
Results: The inclusion criteria were fulfilled by 1842 MA abusers. We found that 770 (41.8%) of MA abusers were assessed with psychiatric symptoms according to ASI. Depression was the most prevalent psychiatric symptom (31.9%), followed by anxiety (24.5%), and psychosis (8.9%). According to the results of multivariate analysis, the demographic profile and the mode of MA administration that are associated with the psychiatric symptoms were: female [odds ratio (OR): 1.95; 95% confidence interval (CI): 1.25–2.96, p<0.001], 5-year duration of use [OR 1.43; 95% CI: 1.43 (1.19–1.73), p<0.001], smoking as the route of administration [OR 1.3; 95% CI: 1.08–1.56, p<0.001, p = 0.006], and concomitant use with other narcotics [OR 1.76; 95% CI: 1.46–2.13, p<0.001].
Conclusion: Among MA abusers admitted to The National Rehabilitation Center, the main factors associated with psychiatric symptoms were: female, long duration of use, smoking as the route of administration, and concomitant use with other narcotics.
PROFILE OF ANTIBIOTIC RESISTANCE AGAINST INFLUENZA IN ADULT PATIENTS: A CASE STUDY AT CITY HEALTH CENTER IN INDONESIA
Authors: RAMDHANI DANNI , KUSUMA SRI AGUNG FITRI , AZIZAH SHINTA NUR , KHUMAIROH IKA , BAP HILARIUS , SEDIANA DEDE 
Published: January 2023

Objective: In developing countries, cases of Haemophilus influenzae (HI) resistance to levofloxacin, cefixime, and tetracycline have become a serious problem in clinical treatment. This study was conducted to determine the antibiotic resistance profile of HI from adult patient isolates and to provide guidelines for more effective clinical treatment in Indonesia.
Methods: The patient isolate stock was rejuvenated, cultured on growth media and the Kirby-Bauer disc diffusion method was used to test for antibiotic susceptibility. Evaluation was guided by recommendations from the Clinical and Laboratory Standard Institute (CLSI).
Results: A total of 643 isolates obtained from the respiratory tract isolated and identified 73 HI strains. The resistance rates of the HI isolates to tetracycline, cefixime, and levofloxacin were 10.54 %, 4.31%, and 5.67%.
Conclusion: Cefixime showed more effective activity than levofloxacin and tetracycline to treat the HI strain.
ETHNOMEDICINAL STUDY OF UBAR KAMPUNG FOR DIABETES MELLITUS: INDIGENOUS KNOWLEDGE, BELIEF, AND PRACTICE OF MEDICINAL, AROMATIC, AND COSMETIC (MAC) PLANTS IN SUNDA REGION, WEST JAVA, INDONESIA
Authors: FEBRIYANTI R. M. , SLIKKERVEER L. J. , SPAINK H. P. , LESTARI K. , SAEFULLAH K. 
Published: January 2023

Objective: Over the past several decades, ethnobotany and ethnomedicine have been considered as a more appropriate interdisciplinary approach to drug discovery involving interdisciplinary collaboration. Several studies on ethnomedicine and ethnobotany worldwide have shown that diabetes mellitus has been recognised by the traditional healer as a pathological condition which can be treated with specific medicinal plants. The starting point in an ethno-directed search for anti-diabetic plants is the identification of plant species used by local healers for this purpose. This study aims to document medicinal plants used by local communities for the treatment of diabetes mellitus.
Methods: This ethnographical study highlights the emic point of the local people to documents knowledge, belief, and practice of ubar kampung in Sunda Region.
Results: The results of this study reveals 20 of most frequently used of medicinal, aromatic, and cosmetic plants by people in Sundanese community. The majority of plant species used for the treatment of diabetes mellitus belong to the families of Asteraceae (2 species), Lauraceae (2 species), and Liliaceae (2 species).
Conclusion: Most of the medicinal plants reported in the research area are already publicly acknowledged for their medicinal properties, indicating that their pharmacological activities have been studied in different areas. Medicinal plants such Syzygium polyanthum, Moringa oleifera, Swietenia mahagoni, Allium sativum, and Cinnammomum burmanni have been widely used in several regions by various ethnic groups. Leaves are the most frequently used plant part. In general, infusion and decoction are the most common plant preparation methods in the research area.
THE POTENTIAL OF LANGIR (ALBIZIA SAPONARIA LOUR.) STEM BARK AS ANTI-DANDRUFF: IN SILICO AND IN VITRO STUDIES
Authors: HIMANIARWATI  , ARBA MUHAMMAD , SUSILAWATI YASMIWAR , MUSTARICHIE RESMI 
Published: January 2023

Objective: Dandruff is a scalp problem that occurs in almost all people in the world. The main cause of dandruff is the growth of fungus on the scalp. This study aims to evaluate the antifungal activity of the Langir bark against Malassezia furfur, in vitro and in silico.
Methods: In vitro testing was carried out using the agar diffusion method with paper disks to calculate the inhibition zones of the Langir stem bark extract and fractions, while the in silico test was carried out using the molecular docking method using Lanosterol 14-alpha demethylase receptors with a homology model using 5 compounds from the genus Albizia as ligands.
Results: Antifungal activity of Langir bark extract showed significant activity in all concentrations (5-20%), and also for all fractions (p<0.05). However, the water fraction had better activity than others, with an inhibition zone of 17.33 mm at a concentration of 15% and 18.67 mm at a concentration of 20%, while the positive control (ketoconazole 1%) had an inhibition zone of 16.67 mm. Furthermore, the in silico test revealed that the 5 metabolites in Langir plant provide better binding energy than ketoconazole (-10.1 kcal/mol), namely, Tamarixetin 3-rutinoside (-10.7 kcal/mol), Quercetin 3-rhamnosyl-galactoside (-10.4 kcal/mol), Albiziasaponin A (-11.6 kcal/mol), Albiziasaponin C (-11.9 kcal/mol) and Albiziasaponin D (-11.9 kcal/mol).
Conclusion: The water fraction of Langir bark has activity in inhibiting the growth of M. furfur so that it can be developed as a therapeutic alternative for anti-dandruff.
CENTRAL COMPOSITE FACE-CENTERED DESIGN-BASED OPTIMISATION, DEVELOPMENT AND CHARACTERISATION OF FAVIPIRAVIR-LOADED PLGA NANOPARTICLES
Authors: R VENKATA KAVYA , B. JEEVANA JYOTHI 
Published: January 2023

Objective: The objective of this study is to fabricate favipiravir-loaded PLGA nanoparticulate systems that can increase the solubility along with the sustained release of favipiravir.
Methods: The favipiravir-loaded Poly (D, L-lactic-co-glycolide) (PLGA) nanoparticulate systems were prepared by the nanoprecipitation method. A 3-factor, 2-level central composite face-centered design was employed to study the effect of formulation variables having a concentration of PLGA, polyvinyl alcohol (PVA) and stirring rate as critical formulation attributes and particle size, drug entrapment efficiency, and percentage cumulative drug release as critical quality attributes on prepared favipiravir nanoparticles. Drug interaction studies were performed by FTIR and DSC. Surface morphology was analysed by scanning electron microscopy (FEI Quanta 250 FEG, USA). Particle size, zeta potential, and polydispersity index were analysed by the nanoparticle analyser SZ-100 (HORIBA Scientific nanopartica, Japan). In vitro drug release studies were performed using a UV-Visible spectrophotometer at λmax 234 nm. In vitro drug release data obtained was fitted into various mathematical kinetic models.
Results: The numerical optimization process predicted the level of PLGA concentration as 69.96 mg, PVA concentration as 4.99%, and stirring rate as 799 rpm for the optimised formulation. The low percentage of relative error for the optimised formulation confirms the validation of the model. The optimised formulation had a 77.65% entrapment efficiency with a particle size of 109.7 nm and the percent cumulative drug release showed 86.46% drug release over 720 min. The drug release was found to follow first-order release kinetics with anomalous non-Fickian diffusion kinetics.
Conclusion: Hence, such an attempt at fabrication of favipiravir-loaded PLGA nanoparticulate systems may be useful for sustained release of drug over 720 min.
AHORRO DE LOS HOGARES MEXICANOS ANTES Y DURANTE LA PANDEMIA DE LA COVID-19
Authors: Villar Hernández Bartolo de Jesús , Jiménez Alba María Guadalupe , Sánchez Carrera Edgar Javier 
Published: January 2023

En este trabajo de investigación analizamos la tasa de ahorro de los hogares mexicanos antes y durante la pandemia por la COVID-19. El objetivo central es actualizar el conocimiento sobre el tema, para así cuantiﬁcar el efecto de las variables socioeconómicas y sociodemográﬁcas determinantes del ahorro. Aplicamos el método econométrico de regresión cuantil para los datos de la Encuesta Nacional de Ingresos y Gastos de los Hogares de 2018 y 2020. Los resultados obtenidos comprueban la heterogeneidad de la inﬂuencia de las variables seleccionadas según el cuantil de la tasa de ahorro modelada, así como el cambio dinámico del efecto asociado a los determinantes y de la tasa de ahorro como consecuencia de la crisis económica derivada por la COVID-19.

ON THE MEXICAN HOUSEHOLDS’ SAVINGS BEFORE AND DURING THE COVID-19 PANDEMIC
ABSTRACT
In this research paper we analyze the Mexican household saving rates before and during the COVID-19 pandemic. Our key research goal is to update the knowledge on this topic and quantify the effects of socioeconomic and sociodemographic covariates as household saving determinants. We apply a quantile regression model taking data from the 2018 and 2020 Encuesta Nacional de Ingresos y Gastos de los Hogares. Our results provide evidence about the heterogeneity of the effects of socioeconomic and sociodemographic household saving determinants, also about the dynamic change of effects associated with the determinants and the household saving rate, as a consequence of the economic crisis derived from COVID-19.