bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of endogenous tolerance to bleaching stress by high salinity in cloned endosymbiotic dinoflagellates from corals
Authors: Chen Ching-Nen Nathan , Yong Tze Ching , Wang Jih-Terng 
Published: January 2025

Abstract

Background
Large-scale coral bleaching events have become increasingly frequent in recent years. This process occurs when corals are exposed to high temperatures and intense light stress, leading to an overproduction of reactive oxygen species (ROS) by their endosymbiotic dinoflagellates. The ROS buildup prompts corals to expel these symbiotic microalgae, resulting in the corals’ discoloration. Reducing ROS production and enhancing detoxification processes in these microalgae are crucial to prevent the collapse of coral reef ecosystems. However, research into the cell physiology and genetics of coral symbiotic dinoflagellates has been hindered by challenges associated with cloning these microalgae.

Results
A procedure for cloning coral symbiotic dinoflagellates was developed in this study. Several species of coral symbionts were successfully cloned, with two of them further characterized. Experiments with the two species isolated from Turbinaria sp. showed that damage from light intensity at 340 μmol photons/m2/s was more severe than from high temperature at 36 °C. Additionally, preincubation in high salinity conditions activated their endogenous tolerance to bleaching stress. Pretreatment at 50 ppt salinity reduced the percentage of cells stained for ROS by 59% and 64% in the two species under bleaching stress compared to those incubated at 30 ppt. Furthermore, their Fv’/Fm’ during the recovery period showed a significant improvement compared to the controls.

Conclusions
These findings suggest that intense light plays a more important role than high temperatures in coral bleaching by enhancing ROS generation in the symbiotic dinoflagellates. The findings also suggest the genomes of coral symbiotic dinoflagellates have undergone evolutionary processes to develop mechanisms, regulated by gene expression, to mitigate damages caused by high temperature and high light stress. Understanding this gene expression regulation could contribute to strengthening corals’ resilience against the impact of global climate change.
Application of physics-informed neural networks (PINNs) solution to coupled thermal and hydraulic processes in silty sands
Authors: Feng Yuan , Eun Jongwan , Kim Seunghee , Kim Yong-Rak 
Published: January 2025

Abstract
The accurate modeling of water and heat transport in soils is crucial for both geo-environmental and geothermal engineering. Traditional modeling methods are problematic because they require well-defined boundaries and initial conditions. Recently, physics-informed neural networks (PINNs), which incorporate partial differential equations (PDEs) to solve forward and inverse problems, have attracted increasing attention in machine learning research. In this study, we applied PINNs to tackle hydraulic and thermal transport coupling forward problems in silty sands. A fully connected deep neural network was utilized for training. This neural network model leverages automatic differentiation to apply the governing equations as constraints, based on the mathematical approximations established by the neural network itself. We conducted forward problems and compared the solutions derived from PINNs with those from Finite Element Method (FEM) simulations. The forward problem results demonstrate the PINNs model’s capability in predicting hydraulic transport, heat transport, and thermal–hydraulic coupling in silty sands under various boundary conditions. The PINNs exhibited great performance in simulating the thermal–hydraulic coupling problem. The accuracy of the PINNs solutions shows its potential for simulation in geotechnical engineering.
Refugee securitization and the challenges of formal integration: case of Somali refugees in Kenya and Ethiopia
Authors: Ikanda Fred N. , Muhumad Abdirahman A. , Kuhnt Jana 
Published: January 2025

Abstract
Securitization of refugees and migrants is a growing global phenomenon that is well illustrated by how refugee camps have emerged as governmental technologies of control in major host countries across the world. Policymakers and the media prominently feature the migration-security connection and depict refugees as threats to internal security, linking their presence to acts of terrorism and crime. This article examines how the securitization of Somali refugees in Kenya and Ethiopia, the two main host countries for this refugee group, has hindered their formal integration and exacerbated policies of encampment in these countries. Drawing on securitization theory, we highlight the discrimination of Somali refugees in Kenya and Ethiopia by demonstrating how this refugee group is seen as a specific security threat and treated differently from other refugee groups in these countries. A history of border conflicts that shaped the relationship between refugee hosting areas and the national governments long before the advent of camps, coupled with shared Somaliness between locals and refugees in hosting areas, and the back-and-forth refugee movements across international borders have created a confluence of factors that reinforce suspicion and distrust towards Somali refugees. The article adds a normative empirical dimension to securitization theory by providing a nuanced understanding of the concept as it applies to different refugee groups in similar contexts. It also contributes to refugee and migration literature by arguing that although shared Somaliness with locals has strengthened the informal integration of Somali refugees in the two study contexts, the same ties have also provided justification for securitizing this refugee group and denied it from benefitting from progressive laws on formal integration that are being implemented for other refugee groups.
CLINTERVENTIONAL protocol: a randomized controlled trial to evaluate clinical consultations and audiovisual tools for interventional radiology
Authors: García Jurado Pedro Blas , Espejo Herrero Juan José , Lombardo Galera María Sagrario , Pérez Montilla María Eugenia , Barranco Acosta Sara , García-Revillo José , Font Ugalde Pilar , Álvarez Benito Marina 
Published: January 2025

Abstract
Interventional radiology (IR) has evolved rapidly, but the clinical integration of interventional radiologists has not kept pace with technical advancements. This trial will address a gap in the literature by providing a robust investigation into specific measures for enhancing the clinical role of interventional radiologists, with potential implications for improving patient experiences and outcomes. The single-center randomized controlled trial will include 428 patients undergoing IR procedures. The control group will receive information about the procedure from the ordering physician, while the experimental group will have an additional consultation with an interventional radiologist and be shown procedure-specific explanatory videos. The primary outcomes are patients’ knowledge, satisfaction with the information and communication, and anxiety. Data collection will involve specific questionnaires and scales. This trial is designed to investigate the importance of proactive clinical roles in patient care within IR. The study explores the potential of consultations and audiovisual tools, highlighting their role in educating patients about procedures. The results may help foster a more widespread acceptance of clinical responsibilities in IR and underscore the pivotal role of audiovisual aids in patient education and satisfaction.

Trial registration
NCT05461482 at clinicaltrials.gov.

Relevance statement
This randomized controlled trial will assess the impact of clinical consultations and explanatory audiovisual tools on patient understanding, satisfaction, and anxiety in interventional radiology. The findings could help establish a more proactive clinical role for interventional radiologists and improve the overall quality of patient-centered care.

Key Points

We describe the protocol of an interventional radiology randomized clinical trial.

The control group will receive procedure information from the referring physician and the experimental group receives additional consultation with interventionalists and views a video.

Knowledge, satisfaction with information, and patient anxiety will be evaluated.

This study will provide insights about the benefits of consultations and videos in interventional radiology.

Graphical Abstract
Asian seabass (Lates calcarifer) larval rearing using cyclopoid copepod (Apocyclops royi) live feed: A new approach
Authors: Vijayaraj Radha , Jayaprakashvel Mani , Altaff Kareem 
Published: January 2025

Abstract

Background
The Apocyclops species are gaining importance as suitable live feed for augmenting growth and survival of marine finfish larvae. In the present study, as a new approach Apocyclops royi is mass cultured using monoalgal diet (Chloroidium saccharophilum) and its nutritive value is assed and used as supplementary diet in the Asian seabass Lates calcarifer larval rearing.

Results
Mass culture of A. royi with C. saccharophilum diet reached population density of 12,700 ± 454 ind./L on 21 days culture period. A. royi showed high ratio of DHA:EPA and EPA: ARA. Rearing experiments of the Asian seabass (Lates. calcarifer) 11 DPH larvae with copepod, Artimia nauplii and their combination diets recorded faster growth and maximum survival (90%) with A. royi + Artemia nauplii diet compared to A. royi and Artemia nauplii lone feeds. Seabass larval length and weight gain showed significant (p < 0.05) difference among the three dietary treatments. Significantly high content of essential fatty acids in the L. calcarifer larvae have been reported in A. royi + Artemia nauplii fed seabass larvae compared to individual A. royi and Artemia nauplii fed larvae.

Conclusions
Our finding highlights the importance of incorporating A. royi as a supplementary feed for seabass larvae, which will bring improvement in their growth and survival leading to sustainable seed production of this fish.
You’ve Got Mail! The Late 19th Century U.S. Postal Service Expansion, Firm Creation, and Firm Performance
Authors: Marinoni Astrid , Roche Maria P. 
Published: January 2025

This paper examines the impact of the expansion of the U.S. Postal Service in the late 19th century on firm creation and performance. Utilizing newly digitized archival data on historic business establishments, post office locations, and road networks in California, our study identifies a positive relationship between the expansion of the Postal Service and the emergence of new firms. To address endogeneity concerns, we leverage an unexpected change in the Postal Service route network. Our findings suggest that the Postal Service played a significant role in facilitating firm entry by acting as a carrier of specialized knowledge rather than as a financial service or mass communication infrastructure. We further reveal that, although increased competition from new entrants generally exerted downward pressure on incumbent firms, those relying on specialized knowledge and public technology inputs significantly benefited from local Postal Service access. Taken together, our study underscores the critical role played by the Postal Service in knowledge diffusion and local economic development by enabling the sourcing of specialized knowledge and technologies from other geographies. Overall, our results contribute to a broader understanding of how communication and knowledge dissemination infrastructure can drive entrepreneurship and firm growth, carrying important implications for contemporary discussions on infrastructure development, its potential to stimulate entrepreneurial activity, innovation, and foster local economic communities. This paper was accepted by Olav Sorenson, organizations. Funding: The authors gratefully acknowledge funding from the Harvard Business School Division of Research and Faculty Development. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2023.03369 .
Dynamic Fair Division with Partial Information
Authors: Benadè Gerdus , Halpern Daniel , Psomas Alexandros 
Published: January 2025

Fair and Efficient Allocation Algorithms Do Not Require Knowing Exact Item Values Food rescue organizations are tasked with allocating often-unpredictable donations to recipients who need it. For a large class of recipient valuation functions, this can be done in a fair and efficient manner as long as each recipient reports their value for each arriving donation. In practice, however, such valuations are rarely elicited. In “Dynamic Fair Division with Partial Information,” Benadè, Halpern, and Psomas ask whether simultaneous fairness and efficiency remain possible when the allocator receives limited information about recipient valuations, even as little as a single binary signal. For recipients with i.i.d. or correlated values, the paper provides an algorithm which is envy-free and 1-epsilon welfare-maximizing with high probability. Asymptotically tight results are also established for independent, nonidentical agents. This shows that fair and efficient online allocation algorithms do not critically rely on recipients being able to precisely report their utility functions.
The impact of reduced skeletal muscle mass at stroke onset on 3-month functional outcomes in acute ischemic stroke patients
Authors: Kim Kyong Young , Jung Seunguk , Cho Eun Bin , Yang Tae-Won , Kim Seung Joo , Kim Hyunsung , Jung Sunhye 
Published: January 2025

Introduction
Sarcopenia, characterized by reduced skeletal muscle mass (RMM), is increasingly recognized as a significant factor influencing outcomes in various health conditions, including stroke. Although most studies focus on sarcopenia developing during stroke rehabilitation, the impact of sarcopenia present at the onset of acute ischemic stroke remains underexplored. This study aims to evaluate the effect of RMM at stroke onset on 3-month functional outcomes in acute ischemic stroke patients.

Materials and methods
We prospectively enrolled acute ischemic stroke patients admitted between May 2019 and December 2019. Muscle mass was accessed early during hospitalization using whole-body dual-energy X-ray absorptiometry (DXA), and patients were categorized into RMM and normal muscle mass (NMM) groups based on the Asian Working Group for Sarcopenia (AWGS) criteria. Functional outcomes at 3 months were assessed using the modified Rankin Scale (mRS), with unfavorable outcomes defined as mRS scores 2–5. Multivariable logistic regression and SHAP (Shapley Additive exPlanations) analyses were used to evaluate the independent impact of RMM on 3-months functional outcomes.

Results
A total of 99 patients were analyzed. The RMM group had a significantly higher prevalence of unfavorable outcomes at 3 months compared to the NMM group (p < 0.001). Patients with RMM were older and presented with more severe strokes. Multivariable analysis confirmed RMM as an independent predictor of unfavorable outcomes (adjusted OR: 8.07, 95% CI: 1.603–40.66, p = 0.011), even after adjusting for age and initial stroke severity. SHAP analysis ranked RMM as the second most influential predictor of unfavorable outcomes, following NIHSS on admission. These findings indicate that RMM not only worsens initial stroke severity but also independently hinders post-stroke recovery.

Conclusions
Reduced muscle mass at the onset of acute ischemic stroke is a significant, independent predictor of unfavorable outcomes at 3 months. In addition to its impact on recovery, RMM is linked to older age and more severe strokes, worsening prognosis. Maintaining muscle mass is also crucial for stroke prevention, as it supports cardiovascular health and resilience. Early identification and intervention for sarcopenia can improve recovery and reduce future stroke risk.
Knowledge of and perception towards eclampsia among women and men in Unguja Island, Zanzibar: A qualitative study
Authors: Yussuf Nassra Is-hak , Stekelenburg Jelle , Mahande Michael Johnson , Manongi Rachel Nathaniel 
Published: January 2025

Background
Eclampsia is among the primary causes of maternal and perinatal morbidity and mortality in Zanzibar. Many women and men are not aware of the signs, symptoms and causes of eclampsia and may have different explanatory models. Therefore, this study aimed to describe the community understanding of pre-eclampsia, as a key stage to improve maternal and perinatal health in Unguja Island, Zanzibar.

Methods
A qualitative study design of six focus group discussions (FDGs) was performed using a focus group interview guide; 51 male and female respondents at three selected wards of Unguja Island, Zanzibar participated. Thematic analysis was applied using Qualitative data analysis (QDA Miner Lite software version 2.0.9).

Results
Overall participants demonstrated a lack of understanding regarding eclampsia. The majority of participants perceived that there are certain things related to eclampsia: Mjusi or Mdudu (devil/satanic), superstitious issues, tension/stress from partners, food consumption, eating slaughtered meat like chicken, cow and goat during pregnancy, nice smelling lotion, soap and perfumes have been stated to be the causes of eclampsia. Spiritual and traditional remedies were mentioned as a treatment for eclampsia, including herbs, makombe (written verses of the Qur’an) and steaming (smoked dry leaves). Local names of eclampsia used by the communities, in urban and rural areas are mjusi (lizard) or mdudu (bug), which means the devil enters a woman’s body, especially during pregnancy or within 42 days post-delivery.

Conclusion
Most participants had a lack of understanding of eclampsia and perceived that there are traditional and spiritual issues related to its causes, attributed risks and clinical presentation, which makes them rely more on traditional and spiritual treatments. Therefore, health educational programs in the community setting and at the antenatal clinics, aiming at improving knowledge and dismissing myths and misperceptions regarding eclampsia, are recommended in rural and urban areas of Unguja Zanzibar.
Associations between the Global Diet Quality Score and risk of type 2 diabetes: Tehran lipid and glucose study
Authors: Daei Shahrzad , Hosseini-Esfahani Firoozeh , Ildarabadi Azam , Mirmiran Parvin , Azizi Fereidoun 
Published: January 2025

Background
Previous studies reported that focusing on healthy lifestyle, especially high diet quality is necessary for preventing type 2 diabetes (T2D). This study investigated the association between the innovative index, the Global Diet Quality Score (GDQS), and the risk of Type 2 Diabetes incidence.

Methods
In this secondary analysis, we included elective adult participants (n = 5948) from the third and fourth survey of the Tehran Lipid and Glucose Study. Participants checked out until the sixth phase with an average follow-up of 6.65 years. Expert nutritionists collected dietary data using a valid and reliable semi-quantitative food frequency questionnaire. The GDQS were calculated, including healthy and unhealthy food group scores. Biochemical and anthropometric characteristics were assessed during the first and follow-up surveys. Multivariable Cox proportional hazards regression models were used to estimate the progression of T2D in association with the GDQS.

Results
This study was implemented on 2,688 men and 3,260 women, respectively with the mean (SD) age of 41.5(14.1) and 39.3(13.02) years. A total of 524 subjects were found to have had T2D incidence. The healthy component of GDQS was conversely associated with T2D incidence [HR: 1, 0.91 (0.84–0.98), 0.91 (0.84–0.98), 0.84 (0.77–0.92) P trend = <0.001] in an adjusted model. The unhealthy component of GDQS was conversely associated with T2D incidence in an adjusted model [HR: 1, 0.86 (0.80–0.92), 0.93 (0.86–1.01), 0.89 (0.81–0.98) P trend = 0.009].

Conclusion
The results of this study suggested that higher adherence to the healthy component of GDQS and lower intake of the unhealthy component decreased the risk of T2D incidence.
Design and fabrication of novel microfluidic-based droplets for drug screening on a chronic myeloid leukemia cell line
Authors: Jaafari Niloofar , Kojabad Amir Asri , Shabestari Rima Manafi , Safa Majid 
Published: January 2025

Background
The challenges associated with traditional drug screening, such as high costs and long screening times, have led to an increase in the use of single-cell isolation technologies. Small sample volumes are required for high-throughput, cell-based assays to reduce assay costs and enable rapid sample processing. Using microfluidic chips, single-cell analysis can be conducted more effectively, requiring fewer reagents and maintaining biocompatibility. Due to the chip’s ability to manipulate small volumes of fluid, high-throughput screening assays can be developed that are both miniaturized and automated. In the present study, we employ microfluidic chips for drug screening in chronic myeloid leukemia. This study aimed to establish a robust methodology integrating diverse assays, providing a holistic understanding of drug response.

Material and methods
Herein, we have used a chronic myeloid leukemia derived cell line (K562) for drug screening with an innovative microfluidic-based drug screening approach to investigate the efficacy of imatinib in K562 cells. Cell viability was assessed using MTT assay. Apoptosis was measured using Annexin/PI staining by flow cytometry.

Results
Significant increased apoptosis was seen in K562 cells treated with imatinib in the microfluidic device compared to cells treated with imatinib in 24- and 96-well plates. Moreover, in the microfluidic chip, drug screening time was reduced from 48 hours to 24 hours.

Conclusion
Compared to traditional approaches, microfluidic-based drug screening efficiently evaluates the efficacy of imatinib in K562 cells. This approach is promising for drug discovery and treatment optimization, as it increases sensitivity and streamlines the screening process.