bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCR-Free Self-Calibrated Ratiometric Electrochemical Genosensor Utilizing a Dual-Signal Amplification Approach for Genomic Detection of Mycobacterium tuberculosis
Authors: BUNYARATAPHAN SASINEE , Prammananan Therdsak 
Published: January 2025

Abstract
Tuberculosis (TB) remains a critical global health challenge. While many electrochemical (EC) biosensors have been developed for Mycobacterium detection, most relied on PCR amplification strategies. This study introduced a PCR-free, self-calibrated ratiometric electrochemical (REC) genosensor leveraging dual-signal amplification for the detection of Mycobacterium tuberculosis (MTB). Electroactive silver nanoparticles (AgNPs) were modified onto screen-printed gold electrodes, followed by chemisorption of a methylene blue (MB)-tagged peptide nucleic acid (PNA) probe targeting MTB genomic DNA. MTB presence triggered structural changes in the probe, reducing the MB redox current (IMB) as the detection signal, while the Ag current (IAg) diminished as an internal calibration marker. This ratiometric mechanism minimized system errors, enhancing detection reliability. The genosensor achieved a broad detection range (3.5 fM–35 nM) with a detection limit of 1.59 fM for non-amplified MTB DNA, showing high specificity, reproducibility, and accuracy comparable to standard diagnostic methods. It effectively detected MTB in simulated sputum samples, demonstrating its potential for reliable, rapid, and amplification-free TB diagnostics.
Multiomics Analysis of Exportin Family Reveals XPO1 as a Novel Target for Clear Cell Renal Cell Carcinoma
Authors: Hao Yanhong , Lv Hongchun , Yan Xu , Liang Yanyan , Jiang Aimin , Zhao Yuxia 
Published: January 2025

Background: Recently, exportin gene family members have been demonstrated to play essential roles in tumor progression. However, research on the clinical significance of exportin gene family members is limited in clear cell renal cell carcinoma (ccRCC).Methods: Pan‐cancer data, ccRCC multiomics data, and single‐cell sequence were included to analyze the differences in DNA methylation modification, single nucleotide variations (SNVs), copy number variations (CNVs), and expression levels of exportin gene family members. Non‐negative matrix factorization was used to identify molecular subtypes based on exportin gene family members, and the prognostic and biological differences of different molecular subtypes were compared across multiple dimensions.Results: Exportin gene family members were upregulated in pan‐cancer expression, and their aberrant expression was significantly influenced by DNA methylation, SNV, and CNV, particularly in ccRCC. Based on the expression matrix of exportin gene family members, two molecular subtypes, exportin famliy genes (XPO)–based subtype 1 (XPS1) and exportin famliy genes (XPO)–based subtype 2 (XPS2), were identified. The expression levels of exportin gene family members in the XPS2 subtype were significantly higher than those in XPS1, and the prognosis was poorer. The XPS2 subtype had lower immune component abundance and higher immune exhaustion scores. Its response rate to immunotherapy was significantly lower than that of the XPS1 subtype, but it was more sensitive to small molecules, including mercaptopurine and nutlin. Among them, exportin‐1 (XPO1) is a potential diagnostic and therapeutic target for ccRCC, which can promote renal cancer progression by activating the PI3K‐AKT‐mTOR (phosphatidylinositol 3‐kinase (PI3K)/AKT serine/threonine kinase (AKT)/mechanistic target of rapamycin (MTOR)) and interferon alpha pathways.Conclusion: This study analyzed the variations of exportin gene family members at the pan‐cancer level and identified two distinct ccRCC subtypes, which can guide personalized management of patients.
Oropharyngeal Cancer Incidence and Trends in Brazil
Authors: Arboleda Lady Paola Aristizabal , de Souza Dyego Leandro Bezerra , Mendonça e Silva Diego Rodrigues , Curado Maria Paula 
Published: January 2025

Abstract

Background:
Oropharyngeal cancer incidence is rising globally, predominantly in high-income countries, because of human papillomavirus infection. However, further data on oropharyngeal cancer incidence in Brazil is needed. The aim of this study was to estimate the incidence, trends, and predictions of oropharyngeal cancer in Brazilian population-based cancer registries (PBCR) by period, sex, and topography.

Methods:
Data on oropharyngeal cancer were collected from PBCRs (1988–2020). Age-standardized rates were calculated from 2000 onward using the 2010 Brazilian census and world standard population. Annual average percent change was analyzed using the joinpoint regression model. Predictions up to 2034 were made using the Nordpred program and the age–period–cohort model.

Results:
A total of 17,980 oropharyngeal cancer cases were recorded across 30 PBCRs (1988–2020). Most cases involved males (81.58%) ages 55 to 59 years (17.06%). The oropharynx not otherwise specified (40.58%), base of the tongue (24.98%), and tonsils (22.52%) were the sites most affected. The highest incidence rates were found in the southeastern and southern regions (3.1–9.4/100,000). Incidence trends increased for 10 PBCR regions in males and 6 regions in females. Predictions up until 2034 indicate decreasing trends for females and increasing trends for males in the north and south of Brazil.

Conclusions:
The incidence of oropharyngeal cancer in Brazil differs among regions, with higher rates observed in the south and southeast. The prevalence of the human papillomavirus-attributable fraction for oropharyngeal cancer is unknown.

Impact:
Analysis of oropharyngeal cancer incidence rates and regional trends aims to better understand the epidemiology of this malignancy in the Brazilian population.
Bone-Induced HER2 Promotes Secondary Metastasis in HR+/HER2− Breast Cancer
Authors: Alam Rahat , Reva Anna , Edwards David G. , Lege Bree M. , Munoz-Arcos Laura S. , Reduzzi Carolina , Singh Swarnima , Hao Xiaoxin , Wu Yi-Hsuan , Tian Zeru , Natalee Laura M. , Damle Gargi , Demircioglu Deniz , Wang Yixian , Wu Ling , Molteni Elisabetta , Hasson Dan , Lim Bora , Gugala Zbigniew , Chipuk Jerry E. , Lang Julie E. , Sparano Joseph A. , Cheng Chonghui , Cristofanilli Massimo , Xiao Han , Zhang Xiang H.-F. , Bado Igor L. 
Published: January 2025

Abstract
Bone metastases can disseminate to secondary sites and promote breast cancer progression, creating additional clinical challenges. The mechanisms contributing to secondary metastasis are barely understood. Here, we evaluate the prediction power of HER2-expressing (HER2E) circulating tumor cells (CTC) after analyzing over 13,000 CTCs from a cohort of 137 patients with metastatic breast cancer with initial HR+/HER2− status and use preclinical models of bone metastasis (BM) to validate the role of HER2E CTCs in multiorgan metastases. Although HER2 expression was higher in patients with BM, experimental analyses revealed that HER2E CTCs derived from bone lesions were more dependent on HER2 activity and more susceptible to anti-HER2 therapy. Targeting the bone-mediated HER2 induction reduces CTC detection and abrogates secondary metastasis from the bone. Overall, we elucidate that HER2E CTCs can serve as a noninvasive biomarker for BM formation with high therapeutic benefit for patients with HR+ metastatic breast cancer.

Significance:
Given the urgent need for alternative strategies to block metastasis progression, we demonstrate that blocking HER2-mediated secondary metastasis improves clinical outcome and establish HER2 as a biomarker for bone metastasis in patients with initial HR+/HER2− breast cancer, which represents ∼70% of all cases.
Impact of Time to Catheter-Based Therapy on Outcomes in Acute Pulmonary Embolism
Authors: Zhang Robert S. , Yuriditsky Eugene , Zhang Peter , Taslakian Bedros , Elbaum Lindsay , Greco Allison A. , Mukherjee Vikramjit , Postelnicu Radu , Amoroso Nancy E. , Maldonado Thomas S. , Horowitz James M. , Bangalore Sripal 
Published: January 2025

BACKGROUND:
The aim of this study was to examine the impact of early versus delayed catheter-based therapies (CBTs) on clinical outcomes in patients with acute intermediate-risk pulmonary embolism (PE).

METHODS:
This retrospective cohort study analyzed data from 2 academic centers involving patients with intermediate-risk PE from January 2020 to January 2024. Patients were divided into early (<12 hours) and delayed CBT (≥12 hours) groups. The primary outcome was a composite of 30-day mortality, resuscitated cardiac arrest, hemodynamic instability, and 90-day readmission. Secondary outcomes included a composite of 30-day mortality, resuscitated cardiac arrest, and hemodynamic instability. Inverse probability of treatment weighting was used to balance covariates.

RESULTS:

A total of 133 patients were included (mean age, 58.3 years; 44% women; 29% catheter-directed thrombolysis; 68% mechanical thrombectomy; and 3% both). The median time to intervention was 6.1 hours in the early group and 20.8 hours in the delayed group (
P
<0.001). A total of 16 patients (12% of patients) experienced the primary composite outcome. Early CBT was associated with a significantly lower rate of the primary composite outcome (4% versus 18%; log-rank
P
<0.001; inverse probability of treatment weighting [hazard ratio, 0.13 (95% CI, 0.03–0.58);
P
=0.007]) and secondary composite outcome (0% versus 9%; log-rank
P
=0.02). The early CBT group also had lower intensive care unit (3.0 versus 3.4 days;
P
=0.01) and hospital length of stay (5.0 versus 6.1 days;
P
=0.046). When patients were stratified by timing of CBT (early/late) and the composite PE shock score (high ≥3; low <3), all 16 patients who experienced the primary composite outcome had a high composite PE shock score, with 14/16 (87.5%) having a high composite PE shock score and delayed intervention.

CONCLUSIONS:
Early CBT was associated with improved clinical outcomes in patients with acute intermediate-risk PE. The composite PE shock score may help identify patients who will benefit from early CBT. Further prospective studies are needed to validate these findings.
Optimal Predilatation Treatment Before Implantation of a Magmaris Bioresorbable Scaffold in Coronary Artery Stenosis: The OPTIMIS Trial
Authors: Hansen Kirstine Nørregaard , Trøan Jens , Maehara Akiko , Noori Manijeh , Hougaard Mikkel , Ellert-Gregersen Julia , Veien Karsten Tange , Junker Anders , Hansen Henrik Steen , Lassen Jens Flensted , Jensen Lisette Okkels 
Published: January 2025

BACKGROUND:
Bioresorbable scaffolds (BRS) were developed to overcome limitations related to late stent failures of drug-eluting stents, but lumen reductions over time after implantation of BRS have been reported. This study aimed to investigate if lesion preparation with a scoring balloon compared with a standard noncompliant balloon minimizes lumen reduction after implantation of a Magmaris BRS assessed with optical coherence tomography and intravascular ultrasound.

METHODS:
Eighty-two patients with stable angina were randomized in a ratio of 1:1 to lesion preparation with a scoring balloon versus a standard noncompliant balloon before implantation of a Magmaris BRS. The primary end point was minimal lumen area at 6-month follow-up.

RESULTS:

Following Magmaris BRS implantation, minimal lumen area (6.4±1.6 versus 6.3±1.5 mm
2
;
P
=0.65), mean scaffold area (7.8±1.5 versus 7.5±1.7 mm
2
;
P
=0.37), and mean lumen area (8.0±1.6 versus 7.7±2.1 mm
2
;
P
=0.41) did not differ significantly in patients with lesions prepared with scoring versus standard noncompliant balloon, respectively. Six-month angiographic follow-up with optical coherence tomography and intravascular ultrasound was available in 74 patients. The primary end point, 6-month minimal lumen area, was significantly larger in lesions prepared with a scoring balloon compared with a standard noncompliant balloon (4.7±1.4 versus 3.9±1.9 mm
2
;
P
=0.04), whereas mean lumen area (7.2±1.4 versus 6.8±2.2 mm
2
;
P
=0.35) did not differ significantly. Intravascular ultrasound findings showed no difference in mean vessel area at the lesion site from baseline to follow-up in the scoring balloon group (16.8±2.9 versus 17.0±3.6 mm
2
;
P
=0.62), whereas mean vessel area (17.1±4.4 versus 15.7±4.9 mm
2
;
P
<0.001) was smaller in lesions prepared with a standard noncompliant balloon due to negative remodeling.

CONCLUSIONS:
Lesion preparation with a scoring balloon before implantation of a Magmaris BRS resulted in a significantly larger minimal lumen area after 6 months due to less negative remodeling compared with lesion preparation with a standard noncompliant balloon.

REGISTRATION:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT04666584.
Language Models and Externalism: A Reply to Mandelkern and Linzen
Authors: Ostertag Gary 
Published: January 2025

Abstract
Do texts generated by language models refer? Mandelkern and Linzen (2024) argue that externalist principles point to an affirmative conclusion. What grounds reference, according to their externalist, is a term’s “natural history”. For example, ‘water’ refers to H2O among English speakers, and not to the phenomenally indistinguishable chemical XYZ, because H2O, and not XYZ, is implicated in the natural history of ‘water’. Appealing to the literature on contrastive explanation, I show that a term’s natural history does not generally ground its referential properties. Thus, Mandelkern and Linzen’s quick route to the referentiality of LM-generated texts fails.
Unveiling Factors Impacting Women’s Positions in Paradiplomacy across Spain’s Autonomous Communities
Authors: Rydzewska Agata 
Published: January 2025

Summary
This study addresses a research gap by integrating a gender perspective into the examination of paradiplomacy. It explores how women are positioned in paradiplomacy and investigates how factors from diplomacy studies impact elected and appointed positions that shape the external actions of sub-state actors. Data collected from paradiplomatic bodies in seventeen Spanish Autonomous Communities reveals a gender composition trend, with men occupying higher-ranking paradiplomatic roles and women more prevalent in less influential positions. Feminist institutionalism is employed to understand gender representation factors and reveal the influence of electoral systems and party ideology. The analysis suggests that men are favoured in challenging positions and prosperous countries, reinforcing gendered assumptions. The study underscores the importance of recognising that all the institutions are gendered and provides insights for further research on gendered institutions in sub-state diplomacy. Despite the limited sample size, the study sheds light on evolving gender patterns within paradiplomacy.
Return to Sport After Distal Biceps Tendon Repair: A Systematic Review
Authors: Boufadel Peter , Daher Mohammad , Lopez Ryan , Fares Mohamad Y. , Lawand Jad , Khan Adam Z. , Abboud Joseph A. 
Published: January 2025

Background: Ruptures of the distal biceps tendon (DBT) can affect the range of motion and strength of the elbow, raising concerns for patients seeking to restore normal function and engage in their regular activities, particularly returning to previous levels of sport participation. Purpose: To characterize and assess the rate and timing of return to sport (RTS) after DBT repair. Study Design: Systematic review; Level of evidence, 4. Methods: PubMed, Embase, and Google Scholar (pages 1-20) were searched from database inception to December 6, 2023 for clinical studies reporting RTS outcomes after DBT repair. The extracted data consisted of patient characteristics; information on the incision approach, fixation method, and rehabilitation protocol; and outcome data including RTS rates, patient-reported outcome measure scores, and complications. Results: A total of 42 studies, including 1093 patients (1100 elbows), met the inclusion criteria. The mean age of patients was 44.9 years, and 99.2% of patients were male. The mean follow-up time was 31.5 months. Ruptures were acute in 87.6% of cases, the dominant arm was injured in 64.9%, and the cause of the injury was sport related in 43.5%. The overall RTS rate was 91.5%, with 85.2% of patients returning to preinjury levels or higher, at a mean time of 6.3 months. Patients had excellent functional outcomes, irrespective of the incision approach or fixation method, although trends associated with a higher RTS rate were observed with bone tunnel fixation, ≤2 weeks of postoperative immobilization, early initiation of active range of motion postoperatively, and initiation of strengthening at ≤10 weeks. Single-incision repair had higher rates of nerve-related complications and reruptures compared with double-incision repair, and cortical button fixation had a higher rate of nerve-related complications among the fixation methods. Conclusion: There was a high rate of RTS after DBT repair at 6 months postoperatively. A positive trend for RTS was observed with respect to rehabilitation protocols favoring earlier active mobility.
Angiosome Territories of Vestibulocochlear Nerve (VIII)
Authors: Simhadri Deepthi , Kanne Haritha , Mallela Upendra 
Published: January 2025

Background Taylor and Palmer introduced an angiosome (vascular) concept in reconstructive plastic surgery in 1987. The angiosome is considered a segment of a nerve (cranial or peripheral nerve) supplied by a primary source of blood vessels. Purpose To observe the arteries supplying the vestibulocochlear nerves (VIII) from the brainstem till their termination. To analyse the neurovascular relationship of the vestibulocochlear nerve. Methods In total, 45 formalin-fixed cadaveric heads with the base of the skull were studied by dissection and histological methods. Results At the level of the pontomedullary junction, it is supplied by the anterior inferior cerebellar artery; within the internal acoustic canal, the cochlear nerve is provided by the cochlear artery, the vestibular nerve is supplied by a vestibular artery, which is the fine branch of the labyrinthine artery. Conclusion Their vascular supply influences the functioning of nerves. The labyrinthine artery is a thin, slender end artery, making it more vulnerable to circulatory disturbances.
Forty years of research trends in long-acting injectable antipsychotics: A bibliometric analysis
Authors: Mert Alper , Soyleyici Mert Tugce 
Published: January 2025

Background Using long-acting injectable (LAI) antipsychotics is crucial for treating psychiatric illnesses, particularly those within the schizophrenia spectrum. Through bibliometric analysis, our study aimed to provide an understanding of the changes in research trends related to LAIs over the past 40 years. Methods We collected the publications from 1983 to 2023 related to research studies on LAIs included in the Web of Science database. Two thousand four hundred and twelve publications were selected based on specific criteria and analyzed using the VOSviewer software and the Biblioshiny app. We obtained and presented data on institutional analysis, country analysis, author and co-authorship analysis, journal analysis, funding agencies, and keyword citation numbers. Results From the period 1983-1992 to 2014-2023, the number of total publications showed a significant growth of 4.91. The majority (approximately 90%) of publications were produced in high-income countries. The private sector may play a significant role in research. The most crucial keywords were schizophrenia and risperidone. Conclusions The trend in LAI research is currently dynamic and ongoing. There seems to be an increasing connection between studies and LAIs that contain second-generation antipsychotics. The number of studies relating to the private sector is noteworthy.
Correlation of King-Devick Test and Helmet Impact Exposures Over a Youth Football Season
Authors: Maynard Jennifer R. , Nadwodny Jeffrey P. , Haak Irvin S. , DeMatas Kristina F. , Rosario-Concepcion Raul A. , Seemann LaRae , Pujalte George G.A. 
Published: January 2025

Background: The cumulative effect of repetitive subconcussive head impacts on neurocognitive function during youth contact sports remains largely unknown. There is a paucity of literature evaluating cumulative helmet forces over a season and their correlation with preseason and postseason cognitive performance tasks such as the King-Devick test (KDT). Hypothesis: Higher helmet forces recorded throughout a 10-week, 10-game youth football season would correlate with slower performance on postseason KDT. Study Design: Prospective cohort study. Level of Evidence: Level 3. Methods: A cohort of 58 youth football players (ages 9-13 years) underwent pre- and postseason KDT. Players wore SpeedFlex helmets (Riddell) fitted with InSite Impact Response System helmet accelerometers (Riddell) which recorded impacts of ≥15 g. Head impacts were tallied over a season and assigned a score of 1, 2, or 3 based on magnitude of g forces. Suspected concussions were correlated with KDT times and recorded instances of head impact. Pre- and postseason KDT scores were compared. Results: During the season, 2013 head impacts were recorded. Median (range) total cumulative force score was 24 (5-476); 6 players sustained head impacts concerning for concussion, and 4 were clinically diagnosed with concussions. Overall, postseason KDT times improved compared with preseason, with a median (range) change of −4.8 seconds (−7.6, −1.1). Analysis showed no correlation between changes in KDT time and total cumulative force score over the season. Conclusion: KDT times in youth football players did not change significantly based on head impact exposure over a single youth football season; most players’ KDT times improved from preseason to postseason. Clinical Relevance: Although our study did not show significant cognitive impact as measured by KDT over a single youth football season, the long-term effects of concussion on the immature brain and how it can impact cognitive development remains largely unknown and should be an area of ongoing study.