bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photocatalytic removal of imidacloprid containing frequently applied insecticide in agriculture industry using Co3O4 modified MoO3 composites
Authors: Adam Mohamed Shaker S. , Sikander Sumbleen , Qamar M. Tariq , Iqbal Shahid , Khalil Ahmed , Taha Amel Musa , Abdel-Rahman Obadah S. , Elkaeed Eslam B. 
Published: March 2023

Water pollution caused by the frequent utilization of pesticides in the agriculture industry is one of the major environmental concerns that require proper attention. In this context, the photocatalytic removal of pesticides from contaminated water in the presence of metallic oxide photocatalysts is quite in approach. In the present study, Orthorhombic MoO3 has been modified with varying amount of cobalt oxide through wet impregnation for the removal of imidacloprid and imidacloprid-containing commercially available insecticide. The solid-state absorption response and band gap evaluation of synthesized composites revealed a significant extension of absorption cross-section and absorption edge in the visible region of the light spectrum than pristine MoO3. The indirect band gap energy varied from ∼2.88 eV (MoO3) to ∼2.15 eV (10% Co3O4-MoO3). The role of Co3O4 in minimizing the photo-excitons’ recombination in MoO3 was studied using photoluminescence spectroscopy. The orthorhombic shape of MoO3 was confirmed through X-ray diffraction analysis and scanning electron microscopy. Moreover, the presence of distinct absorption edges and diffraction peaks corresponding to Co3O4 and MoO3 in absorption spectra and XRD patterns, respectively verified the composite nature of 10% Co3O4-MoO3. The photocatalytic study under natural sunlight irradiation showed higher photocatalytic removal (∼98%) of imidacloprid with relatively higher rate by 10% Co3O4-MoO3 composite among all contestants. Furthermore, the photocatalytic removal (∼93%) of commercially applied insecticide, i.e., Greeda was also explored.
Sciatic nerve fractional anisotropy and neurofilament light chain protein are related to sensorimotor deficit of the upper and lower limbs in patients with type 2 diabetes
Authors: Kender Zoltan , Jende Johann M. E. , Kurz Felix T. , Tsilingiris Dimitrios , Schimpfle Lukas , Sulaj Alba , von Rauchhaupt Ekaterina , Bartl Hannelore , Mooshage Christoph , Göpfert Jens , Nawroth Peter , Herzig Stephan , Szendroedi Julia , Bendszus Martin , Kopf Stefan 
Published: March 2023

BackgroundDiabetic sensorimotor polyneuropathy (DSPN) is one of the most prevalent and poorly understood diabetic microvascular complications. Recent studies have found that fractional anisotropy (FA), a marker for microstructural nerve integrity, is a sensitive parameter for the structural and functional nerve damage in DSPN. The aim of this study was to investigate the significance of proximal sciatic nerve’s FA on different distal nerve fiber deficits of the upper and lower limbs and its correlation with the neuroaxonal biomarker, neurofilament light chain protein (NfL).Materials and methodsSixty-nine patients with type 2 diabetes (T2DM) and 30 healthy controls underwent detailed clinical and electrophysiological assessments, complete quantitative sensory testing (QST), and diffusion-weighted magnetic resonance neurography of the sciatic nerve. NfL was measured in the serum of healthy controls and patients with T2DM. Multivariate models were used to adjust for confounders of microvascular damage.ResultsPatients with DSPN showed a 17% lower sciatic microstructural integrity compared to healthy controls (p&lt;0.001). FA correlated with tibial and peroneal motor nerve conduction velocity (NCV) (r=0.6; p&lt;0.001 and r=0.6; p&lt;0.001) and sural sensory NCV (r=0.50; p&lt;0.001). Participants with reduced sciatic nerve´s FA showed a loss of function of mechanical and thermal sensation of upper (r=0.3; p&lt;0.01 and r=0.3; p&lt;0.01) and lower (r=0.5; p&lt;0.001 and r=0.3; p=&lt;0.01) limbs and reduced functional performance of upper limbs (Purdue Pegboard Test for dominant hand; r=0.4; p&lt;0.001). Increased levels of NfL and urinary albumin-creatinine ratio (ACR) were associated with loss of sciatic nerve´s FA (r=-0.5; p&lt;0.001 and r= -0.3, p= 0.001). Of note, there was no correlation between sciatic FA and neuropathic symptoms or pain.ConclusionThis is the first study showing that microstructural nerve integrity is associated with damage of different nerve fiber types and a neuroaxonal biomarker in DSPN. Furthermore, these findings show that proximal nerve damage is related to distal nerve function even before clinical symptoms occur. The microstructure of the proximal sciatic nerve and is also associated with functional nerve fiber deficits of the upper and lower limbs, suggesting that diabetic neuropathy involves structural changes of peripheral nerves of upper limbs too.
Potentially suitable habitat prediction of Pinus massoniana Lamb. in China under climate change using Maxent model
Authors: Chi Yi , Wang G. Geoff , Zhu Mengxun , Jin Peng , Hu Yue , Shu Pengzhou , Wang Zhongxu , Fan Aifei , Qian Penghong , Han Yini , Jin Songheng 
Published: March 2023

BackgroundPinus massoniana is an important timber species with high ecological and economic value in southern China and a pioneer species for the reforestation of barren mountains. The structure and function of the existing Masson pine ecosystem have been seriously affected by worsening habitats under current climate change. Its suitable habitat is likely to change greatly in the near future.MethodsTo estimate the potential geographic distribution of P. massoniana and its response to climate change, the Maxent model was selected to simulate the potentially suitable habitat and corresponding changes in the distribution pattern of P. massoniana under current and future climate scenarios (SSP1-2.6, SSP2-4.5, and SSP5-8.5) with two periods (2050s and 2090s).ResultsUnder current climate conditions, the total suitable habitat area of P. massoniana was 2.08 × 106 km2, including 0.76 × 106 km2 of highly suitable habitat, concentrated mainly in Jiangxi and Zhejiang, central and southeastern Hunan, northern Fujian, central and western Chongqing, southern Anhui, central and surrounding areas of Guangdong, central and eastern Guangxi, and northern Taiwan. The areas of moderately suitable habitat and poorly suitable habitat were 0.87 × 106 km2 and 0.45 × 106 km2, respectively. Temperature and precipitation appear to be the most important predictors: precipitation of the driest month (14.7–215.6 mm), minimum temperature of the coldest month (−3.5–13.8°C), annual temperature range (8.1–32.9°C), and mean temperature of the warmest quarter (23.6–34.7°C). P. massoniana are predicted to expand their potential distribution under future climate change: by the end of this century, their total suitable habitat area increased 0.26 × 106 km2 (10.61%) and 0.45 × 106 km2 (17.05%) under the most moderate (SSP1-2.6) and severe (SSP5-8.5) warming scenarios, respectively, by mainly extending northward.ConclusionUnder the different future climate scenarios, the total suitable habitat area of P. massoniana increased by mainly extending northward. Overall, our study clarifies the potential habitat distribution of P. massoniana and provides a critical empirical reference for future P. massoniana conservation and planting practices.
Construction of a prognostic model based on eight ubiquitination-related genes via machine learning and potential therapeutics analysis for cervical cancer
Authors: Hao Yiping , Guy Mutangala Muloye , Liu Qingqing , Li Ruowen , Mao Zhonghao , Jiang Nan , Wang Bingyu , Cui Baoxia , Zhang Wenjing 
Published: March 2023

Introduction: Ubiquitination is involved in many biological processes and its predictive value for prognosis in cervical cancer is still unclear.Methods: To further explore the predictive value of the ubiquitination-related genes we obtained URGs from the Ubiquitin and Ubiquitin-like Conjugation Database, analyzed datasets from The Cancer Genome Atlas and Gene Expression Omnibus databases, and then selected differentially expressed ubiquitination-related genes between normal and cancer tissues. Then, DURGs significantly associated with overall survival were selected through univariate Cox regression. Machine learning was further used to select the DURGs. Then, we constructed and validated a reliable prognostic gene signature by multivariate analysis. In addition, we predicted the substrate proteins of the signature genes and did a functional analysis to further understand the molecular biology mechanisms. The study provided new guidelines for evaluating cervical cancer prognosis and also suggested new directions for drug development.Results: By analyzing 1,390 URGs in GEO and TCGA databases, we obtained 175 DURGs. Our results showed 19 DURGs were related to prognosis. Finally, eight DURGs were identified via machine learning to construct the first ubiquitination prognostic gene signature. Patients were stratified into high-risk and low-risk groups and the prognosis was worse in the high-risk group. In addition, these gene protein levels were mostly consistent with their transcript level. According to the functional analysis of substrate proteins, the signature genes may be involved in cancer development through the transcription factor activity and the classical P53 pathway ubiquitination-related signaling pathways. Additionally, 71 small molecular compounds were identified as potential drugs.Conclusion: We systematically studied the influence of ubiquitination-related genes on prognosis in cervical cancer, established a prognostic model through a machine learning algorithm, and verified it. Also, our study provides a new treatment strategy for cervical cancer.
Accelerated institutionalization of an adolescent sexual and reproductive health (ASRH) intervention in Tanzania: Findings from a mixed-methods evaluation
Authors: Cutherell Meghan , Bwire Juliana , Mtei Edwin , Musau Abednego , Kahabuka Catherine , Luhanga Isabellah , Julius Augustino , Kihwele Gerald 
Published: March 2023

IntroductionFrom 2018 to 2020, Adolescents 360 (A360), aiming to increase demand for and voluntary uptake of modern contraception among adolescent girls 15–19 years, designed and scaled an intervention in Tanzania (Kuwa Mjanja) to 13 regions through project-funded expansion. In 2020, the project began to develop a strategy for its follow-on phase, focusing on program sustainability. In this process, funder priorities led to a decision to exit A360's programming in Tanzania over a 15-month exit period. A360 elected to pursue a process of expedited institutionalization of Kuwa Mjanja into government systems during this period.Materials and methodsThe institutionalization process was facilitated in 17 local government authorities in Tanzania. Quantitative and qualitative data were gathered and analyzed including time-trend analysis of routine performance data, statistical analysis of two rounds of client exit interviews, and thematic analysis of qualitative research.ResultsThe sociodemographic characteristics of adolescent girls reached under government-led implementation were comparable to those reached by A360-led implementation. Intervention productivity decreased under government-led implementation but remained consistent. Adopter method mix shifted slightly toward greater long-acting and reversible contraceptive uptake under a government-led model. Factors that enabled successful institutionalization of Kuwa Mjanja included the presence of youth-supportive policies, the establishment of school clubs which provided sexual and reproductive health education, commitment of government stakeholders, and appreciation of adolescent pregnancy as a problem. Some intervention components were important for program effectiveness but proved difficult to institutionalize, primarily because of resource constraints. Lack of adolescent sexual and reproductive health (ASRH)-focused targets and indicators disincentivized Kuwa Mjanja implementation.DiscussionThere is significant potential in operationalizing user-centered ASRH models within government structures, even in a narrow time frame. A360 saw similar performance under government-led implementation and fidelity to the unique experience that the program was designed to deliver for adolescent girls. However, beginning this process earlier presents greater opportunities, as some aspects of the institutionalization process that are critical to sustained impact, for example, shifting government policy and measurement and mobilizing government resources, require heavy coordination and long-term efforts. Programs pursuing institutionalization in a shorter time frame would benefit from setting realistic expectations. This may include prioritizing a smaller subset of program components that have the greatest impact.
Causal associations between modifiable risk factors and pancreatitis: A comprehensive Mendelian randomization study
Authors: Mao Xiaotong , Mao Shenghan , Sun Hongxin , Huang Fuquan , Wang Yuanchen , Zhang Deyu , Wang Qiwen , Li Zhaoshen , Zou Wenbin , Liao Zhuan 
Published: March 2023

BackgroundThe pathogenesis of pancreatitis involves diverse environmental risk factors, some of which have not yet been clearly elucidated. This study systematically investigated the causal effects of genetically predicted modifiable risk factors on pancreatitis using the Mendelian randomization (MR) approach.MethodsGenetic variants associated with 30 exposure factors were obtained from genome-wide association studies. Summary-level statistical data for acute pancreatitis (AP), chronic pancreatitis (CP), alcohol-induced AP (AAP) and alcohol-induced CP (ACP) were obtained from FinnGen consortia. Univariable and multivariable MR analyses were performed to identify causal risk factors for pancreatitis.ResultsGenetic predisposition to smoking (OR = 1.314, P = 0.021), cholelithiasis (OR = 1.365, P = 1.307E-19) and inflammatory bowel disease (IBD) (OR = 1.063, P = 0.008) as well as higher triglycerides (OR = 1.189, P = 0.016), body mass index (BMI) (OR = 1.335, P = 3.077E-04), whole body fat mass (OR = 1.291, P = 0.004) and waist circumference (OR = 1.466, P = 0.011) were associated with increased risk of AP. The effect of obesity traits on AP was attenuated after correcting for cholelithiasis. Genetically-driven smoking (OR = 1.595, P = 0.005), alcohol consumption (OR = 3.142, P = 0.020), cholelithiasis (OR = 1.180, P = 0.001), autoimmune diseases (OR = 1.123, P = 0.008), IBD (OR = 1.066, P = 0.042), type 2 diabetes (OR = 1.121, P = 0.029), and higher serum calcium (OR = 1.933, P = 0.018), triglycerides (OR = 1.222, P = 0.021) and waist-to-hip ratio (OR = 1.632, P = 0.023) increased the risk of CP. Cholelithiasis, triglycerides and the waist-to-hip ratio remained significant predictors in the multivariable MR. Genetically predicted alcohol drinking was associated with increased risk of AAP (OR = 15.045, P = 0.001) and ACP (OR = 6.042, P = 0.014). After adjustment of alcohol drinking, genetic liability to IBD had a similar significant causal effect on AAP (OR = 1.137, P = 0.049), while testosterone (OR = 0.270, P = 0.002) a triglyceride (OR = 1.610, P = 0.001) and hip circumference (OR = 0.648, P = 0.040) were significantly associated with ACP. Genetically predicted higher education and household income levels could lower the risk of pancreatitis.ConclusionsThis MR study provides evidence of complex causal associations between modifiable risk factors and pancreatitis. These findings provide new insights into potential therapeutic and prevention strategies.
Bioengineered bacterial outer membrane vesicles encapsulated Polybia–mastoparan I fusion peptide as a promising nanoplatform for bladder cancer immune-modulatory chemotherapy
Authors: Ren Chunyu , Li Yangyang , Cong Zhaoqing , Li Zhuoran , Xie Leiming , Wu Song 
Published: March 2023

BackgroundNanosized bacterial outer membrane vesicles (OMVs) secreted by Gram-negative bacteria have emerged as a novel antitumor nanomedicine reagent due to their immunostimulatory properties. The encapsulated bacterial composition in OMVs can be edited via manipulating bioengineering technology on paternal bacteria, allowing us to design an ingenious antitumor platform by loading the Polybia–mastoparan I (MPI) fusion peptide into OMVs.MethodsOMVs containing the MPI fusion peptide were obtained from bioengineered Escherichia coli transformed with recombinant plasmid. The antitumor efficacy of bioengineered OMVs in vitro was verified by performing cell viability and wound-healing and apoptosis assays using MB49 and UMUC3 cells, respectively. Subcutaneous MB49 tumor-bearing mice were involved to investigate the tumor inhibition ability of bioengineered OMVs. Moreover, the activated immune response in tumor and the biosafety were also evaluated in detail.ResultsThe resulting OMVs had the successful encapsulation of MPI fusion peptides and were subjected to physical characterization for morphology, size, and zeta potential. Cell viabilities of bladder cancer cells including MB49 and UMUC3 rather than a non-carcinomatous cell line (bEnd.3) were decreased when incubated with bioengineered OMVs. In addition, bioengineered OMVs restrained migration and induced apoptosis of bladder cancer cells. With intratumor injection of bioengineered OMVs, growths of subcutaneous MB49 tumors were significantly restricted. The inherent immunostimulation of OMVs was demonstrated to trigger maturation of dendritic cells (DCs), recruitment of macrophages, and infiltration of cytotoxic T lymphocytes (CTLs), resulting in the increased secretion of pro-inflammatory cytokines (IL-6, TNF-α, and IFN-γ). Meanwhile, several lines of evidence also indicated that bioengineered OMVs had satisfactory biosafety.ConclusionBioengineered OMVs fabricated in the present study were characterized by strong bladder cancer suppression and great biocompatibility, providing a new avenue for clinical bladder cancer therapy.
Low-dose administration of prednisone has a good effect on the treatment of prolonged hematologic toxicity post-CD19 CAR-T cell therapy
Authors: Wang Jiaxi , Zhang Meng , Lyu Hairong , Guo Ruiting , Xiao Xia , Bai Xue , Pu Yedi , Meng Juanxia , Li Qing , Yuan Ting , Lu Wenyi , Zhao Mingfeng 
Published: March 2023

IntroductionHematologic toxicity (HT) is a joint adverse event after CAR-T cells infusion. Some patients experience prolonged hematologic toxicity (PHT), which is challenging to treat.MethodsWe collected clinical data from patients with relapsed refractory B-ALL treated with CD19 CAR-T cells. Patients with PHT who did not respond to erythropoietin, platelet receptor agonists, transfusion, or G-CSF and eventually received low-dose prednisone therapy were included in the analysis. We retrospectively analyzed the efficacy and safety of low-dose prednisone on PHT.ResultsAmong 109 patients treated with CD19 CAR-T cells, 78.9% (86/109) of patients were evaluated as PHT. Of these, 15 patients had persistent hematological toxicity after infusion (12 were grade 3/4 cytopenia, 12 were trilineage cytopenia and 3 were bilineage cytopenia), 2 developed cytopenia without apparent cause after D28. The initial prednisone dose was 0.5 mg/kg/day, and the median response time was 21 days (7-40 days). The recovery rate of blood count was 100%, and the complete recovery rate ranged from 60% to 66.67%. Especially exciting was that HT recurred in 6 patients after stopping prednisone. They were relieved again after the administration of prednisone. The median follow-up time was 14.97 months (4.1-31.2 months). Twelve-month duration of PFS and OS rates were 58.8% (±11.9%) and 64.7% (±11.6%). We did not observe any other side effects of prednisone apart from drug-controllable hyperglycemia and hypertension.DiscussionWe suggest that low-dose prednisone is a beneficial and tolerable therapy for PHT after CAR-T cells. The trials have been registered at www.chictr.org.cn as ChiCTR-ONN-16009862 (November 14, 2016) and ChiCTR1800015164 (March 11, 2018).
Warm needle acupuncture for osteoarthritis: An overview of systematic reviews and meta-analysis
Authors: Jun Ji Hee , Choi Tae-Young , Park Sunju , Lee Myeong Soo 
Published: March 2023

BackgroundOsteoarthritis (OA) is a chronic disease that is a major cause of pain and functional disability. Warm needle acupuncture (WA) therapy has been widely used to treat OA. This overview summarizes the evidence from systematic reviews (SRs) and assesses the methodological quality of previous SRs that evaluated the use of WA therapy for OA.MethodsWe searched electronic databases to identify SRs that evaluated the efficacy of WA therapy for OA. Two reviewers independently extracted data and assessed the methodological quality of the reviews according to the A Measurement Tool to Assess Systematic Reviews (AMSTAR 2) tool. The reporting quality was assessed using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis 2020 (PRISMA 2020) guidelines. The quality of evidence was assessed according to the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach.ResultsFifteen SRs were included in this study. WA therapy was more effective than control conditions for the treatment of OA. The results of the AMSTAR 2 tool showed that the methodological quality of all included studies was critically low. The items with the lowest scores were item 2 (reporting the protocol), item 7 (listing excluded studies and justifying the exclusions), and item 16 (including conflicts of interest). Regarding the PRISMA guidelines, 2 SRs exhibited greater than 85% compliance. The overall quality of evidence in the included SRs ranged from “very low” to “moderate.”ConclusionThis overview shows that WA therapy was more effective than the control treatment for OA. However, the methodological quality of the reviews was low, indicating the need for improvements in the collection of evidence. Future studies are needed to collect high-quality evidence regarding the use of WA for OA.Systematic review registrationhttps://www.researchregistry.com/, Research Registry (reviewregistry1317).