bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water stress tolerance of several newly derived breadfruit cultivars
Authors: Martirosyan H , Nikkhahkouchaksaraei H , Sadoyan R , Avetisyan A 
Published: March 2023

Abstract
To evaluation of several wild wheat and barley genotypes in response to water deficit stress, experiments were laid out in randomized complete block design with three replicates in optimal and stress condition environment for three years. Data analysis showed a significant genetic diversity within genotypes in terms of grain yield, and the existence of significant genotype × environment interaction, made it possible to analyze data for genotypes stability to environment in this study. The stability analysis showed that Garni (an emmer variety) was recognized as the most stable genotype to water deficit stress with a high grain yield. The stress tolerance tests also found that the Garni genotype is more tolerant to water deficit stress than other genotypes. Based on the results obtained, Garni genotype was recognized as a stable genotype and the most tolerant genotype to water deficit stress that has a high grain yield, and it can be used as a crossbreeding parent in wheat breeding.
Design of a small-scale wind tunnel for the study of horizontal-axis wind turbine blades
Authors: Pankratov E V , Kangash A I , Kosarev I A , Maryandyshev P A 
Published: March 2023

Abstract
The paper considers the details of design and construction of a small-sized wind tunnel with an open circuit and a closed test section for investigating the blades of horizontal-axis wind turbines. The effect of different types of jet straighteners on the turbulence of the flow in the test section has been studied. Turbulence flow distributions Tu at Reynolds numbers Re from 5·103 to 2·104 are obtained using Laser Doppler Anemometry methods. As a result, the wind tunnel design with average flow turbulence of 1.6 % is obtained. The flow visualization of NACA 64-618 airfoil at Re = 5·103 is obtained.
An evaluation on mechanical properties of vaccum bagging processed kenaf epoxy nano aluminium oxide and nanographene composites
Authors: Taj Amreen , R P Swamy , Naik Kishan , K N Bharath 
Published: March 2023

Abstract
This paper aims to study the mechanical properties of hybrid polymer composites and the addition of primary and secondary fillers the nano-aluminum oxide and nanographene to the Kenaf epoxy composites. The vacuum bagging method was used to fabricate the composites. The advantage of using vacuum bagging is to reduce blowholes. The weight ratio of Kenaf fiber 50%–60%, matrix 40%, fillers nano-aluminum oxide 0%–6%, and nanographene 0%–3% was considered. Mechanical properties like Tensile strength, Young’s modulus and elongation, flexural strength, flexural modulus, hardness, impact strength, and compression strength were tested.6% nano aluminum oxide and 3% nanographene show a 125% increase in tensile strength, a 45% increase in flexural strength, and almost four times higher flexural modulus recorded. 42% increase in compression strength, and an increase of 40% in impact strength. The highest Vickers hardness is 27. An increase in primary and secondary fillers decreases and a combination of increases the mechanical properties.
An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms
Authors: Laily Izza Nur , Takeuchi Michiki , Mizutani Taku , Ogawa Jun 
Published: March 2023

ABSTRACT
Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in Escherichia coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7, and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme.
Impact of procedure type on revisional surgery and secondary reconstruction after immediate breast reconstruction in a population-based cohort
Authors: Johnson Leigh , White Paul , Holcombe Chris , O’Donoghue Joe , Jeevan Ranjeet , Browne John , Fairbrother Patricia , MacKenzie Mairead , Gulliver-Clarke Carmel , Mohiuddin Syed , Hollingworth Will , Potter Shelley 
Published: March 2023

Abstract

Background
Women considering immediate breast reconstruction require high-quality information about the likely need for secondary reconstruction and the long-term risk of revisional surgery to make fully informed decisions about different reconstructive options. Such data are currently lacking. This study aimed to explore the impact of reconstruction type on the number of revisions and secondary reconstructions performed 3, 5, and 8 years after immediate breast reconstruction in a large population-based cohort.

Methods
Women undergoing unilateral mastectomy and immediate breast reconstruction for breast cancer or ductal carcinoma in situ in England between 1 April 2009 and 31 March 2015 were identified from National Health Service Hospital Episode Statistics. Numbers of revisions and secondary reconstructions in women undergoing primary definitive immediate breast reconstruction were compared by procedure type at 3, 5, and 8 years after index surgery.

Results
Some 16 897 women underwent immediate breast reconstruction with at least 3 years’ follow-up. Of these, 14 069 had a definitive reconstruction with an implant only (5193), latissimus dorsi flap with (3110) or without (2373) an implant, or abdominal free flap (3393). Women undergoing implant-only reconstruction were more likely to require revision, with 69.5 per cent (747 of 1075) undergoing at least one revision by 8 years compared with 49.3 per cent (1568 of 3180) in other reconstruction groups. They were also more likely to undergo secondary reconstruction, with the proportion of women having further reconstructive procedures increasing over time: 12.8 per cent (663 of 5193) at 3 years, 14.3 per cent (535 of 3752) at 5 years, and 17.6 per cent (189 of 1075) at 8 years.

Conclusion
Long-term rates of revisions and secondary reconstructions were considerably higher after primary implant-based reconstruction than autologous procedures. These results should be shared with patients to support informed decision-making.
Cognitive training, but not EEG-neurofeedback, improves working memory in healthy volunteers
Authors: Barbazzeni Beatrice , Speck Oliver , Düzel Emrah 
Published: March 2023

AbstractWorking memory performance can be influenced by motivational factors, which may be associated with specific brain activities, including suppression of alpha oscillations. We investigated whether providing individuals online feedback about their ongoing oscillations (EEG-neurofeedback) can improve working memory under high and low reward expectancies. We combined working memory training with neurofeedback to enhance alpha suppression in a monetary-rewarded delayed match-to-sample task for visual objects. Along with alpha, we considered the neighbouring theta and beta bands. In a double-blind experiment, individuals were trained over 5 days to suppress alpha power by receiving real-time neurofeedback or control neurofeedback (placebo) in reward and no-reward trials. We investigated (i) whether neurofeedback enhances alpha suppression, (ii) whether monetary reward enhances alpha suppression and working memory, and (iii) whether any performance benefits of neurofeedback-training would transfer to unrelated cognitive tasks. With the same experimental design, we conducted two studies with differing instructions given at the maintenance, yielding together 300 EEG recording sessions. In Study I, participants were engaged in a mental calculation task during maintenance. In Study II, they were instructed to visually rehearse the sample image. Results from Study I demonstrated a significant training and reward-anticipation effect on working memory accuracy and reaction times over 5 days. Neurofeedback and reward anticipation showed effects on theta suppression but not on alpha suppression. Moreover, a cognitive training effect was observed on beta suppression. Thus, neurofeedback-training of alpha was unrelated to working memory performance. Study II replicated the training and reward-anticipation effect on working memory but without any effects of neurofeedback-training on oscillations or working memory. Neither study showed transfer effects of either working memory or neurofeedback-training. A linear mixed-effect model analysis of neurofeedback-independent training-related improvement of working memory combining both studies showed that improved working memory performance was related to oscillatory changes over training days in the encoding and maintenance phases. Improvements in accuracy were related to increasing beta amplitude in reward trials over right parietal electrodes. Improvements in reaction times were related to increases in right parietal theta amplitude during encoding and increased right parietal and decreased left parietal beta amplitudes during maintenance. Thus, while our study provided no evidence that neurofeedback targeting alpha improved the efficacy of working memory training or evidence for transfer, it showed a relationship between training-related changes in parietal beta oscillations during encoding and improvements in accuracy. Right parietal beta oscillations could be an intervention target for improving working memory accuracy.
PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis
Authors: Chen Wen , He Qunjun , Liu Jingjing , Li Ni , Xiao Kai , Chen Honghui 
Published: March 2023

Abstract

Objectives
Although great progress has made in gastric cancer (GC) in the past years, the overall 5-year survival rate remains to be low for advanced GC patients. A recent study showed that PLAGL2 was increased in GC and enhanced the proliferation and metastasis of GC. Nevertheless, the underlying mechanism still needs to be investigated.

Methods
Gene and protein expressions were assessed using RT-qPCR and western blot. The migration, proliferation and invasion of GC cells were examined using scratch assay, CCK-8 assay and Transwell assay, respectively. ChIP-PCR, dual-luciferase assay, RIP-qPCR and CoiP were utilized to confirm the interaction among PLAGL2, UCA1, miR-145-5p and YTHDF1 as well as METTL3, YTHDF1 and eEF-2. A mouse xenograft model was used utilized to further confirm the regulatory network.

Results
PLAGL2 bound to the upstream promoter of UCA1, which regulated YTHDF1 by sponging miR-145-5p. METTL3 can mediate the m6A modification level of Snail. YTHDF1 recognized m6A-modified Snail by interacting with eEF-2 and thus promoted Snail expression, which eventually induced epithelial-mesenchymal transition (EMT) in GC cells and metastasis of GC.

Conclusions
Overall, our study demonstrates that PLAGL2 enhances Snail expression and GC progression via the UCA1/miR-145-5p/YTHDF1 axis, suggesting that PLAGL2 may become a therapeutic target for GC treatment.
Paediatric dermatology teleconsultations: a survey of healthcare professionals in the UK
Authors: McCusker Sarah , King Stokes Natalie , Hunjan Manrup , Daly Aoife , George Susannah , Solman Lea , Burden-Teh Esther 
Published: March 2023

Abstract
The COVID-19 pandemic catapulted dermatology services into a digital era, with the rapid introduction of teleconsultations. The UK National Health Service operational planning guidance recommends ≥ 25% of consultations are delivered remotely. There is a lack of data regarding the acceptability and effectiveness of paediatric dermatology teleconsultations. We surveyed UK healthcare professionals (HCPs) to explore their experiences of teleconsultations in paediatric dermatology, with a focus on follow-up consultations for paediatric eczema (PE), to inform a future clinical trial. There were 119 responses. Pre-pandemic, 37% provided some form of teleconsultation service, rising to 92% post-pandemic. In total, 41% (n = 49) now carry out &gt; 25% of consultations remotely. We found 55% felt teleconsultations were less effective than face-to-face ones for PE follow-up. Eighty HCPs offered teleconsultations for PE. Among the HPCs who offered teleconsultations for PE, the most effective format for follow-up consultations was felt to be telephone with photographs (52/80, 65%). Our results demonstrate varying opinion on the effectiveness and optimal format of paediatric teleconsultations, supporting the need for further research.
Evaluation of Acebilustat, a Selective Inhibitor of Leukotriene B4 Biosynthesis, for Treatment of Outpatients With Mild-Moderate Coronavirus Disease 2019: A Randomized, Double-Blind, Placebo-Controlled Phase 2 Trial
Authors: Levitt Joseph E , Hedlin Haley , Duong Sophie , Lu Di , Lee Justin , Bunning Bryan , Elkarra Nadia , Pinsky Benjamin A , Heffernan Eileen , Springman Eric , Moss Richard B , Bonilla Hector F , Parsonnet Julie , Zamanian Roham T , Langguth Jamison J , Bollyky Jenna , Khosla Chaitan , Nicolls Mark R , Desai Manisha , Rogers Angela J 
Published: March 2023

Abstract

Background
The vast majority of coronavirus disease 2019 (COVID-19) disease occurs in outpatients where treatment is limited to antivirals for high-risk subgroups. Acebilustat, a leukotriene B4 inhibitor, has potential to reduce inflammation and symptom duration.

Methods
In a single-center trial spanning Delta and Omicron variants, outpatients were randomized to 100 mg/d of oral acebilustat or placebo for 28 days. Patients reported daily symptoms via electronic query through day 28 with phone follow-up on day 120 and collected nasal swab samples on days 1–10. The primary outcome was sustained symptom resolution to day 28. Secondary 28-day outcomes included time to first symptom resolution, area under the curve (AUC) for longitudinal daily symptom scores, duration of viral shedding through day 10, and symptoms on day 120.

Results
Sixty participants were randomized to each study arm. At enrollment, the median duration was 4 days (interquartile range, 3–5 days), and the median number of symptoms was 9 (7–11). Most patients (90%) were vaccinated, with 73% having neutralizing antibodies. A minority of participants (44%; 35% in the acebilustat arm and 53% in placebo) had sustained symptom resolution at day 28 (hazard ratio, 0.6 [95% confidence interval, .34–1.04]; P = .07 favoring placebo). There was no difference in the mean AUC for symptom scores over 28 days (difference in mean AUC, 9.4 [95% confidence interval, −42.1 to 60.9]; P = .72). Acebilustat did not affect viral shedding or symptoms at day 120.

Conclusions
Sustained symptoms through day 28 were common in this low-risk population. Despite this, leukotriene B4 antagonism with acebilustat did not shorten symptom duration in outpatients with COVID-19.
Clinical Trials Registration. NCT04662060.
Role of Cerebral Imaging on Diagnosis and Management in Patients With Suspected Infective Endocarditis
Authors: Papadimitriou-Olivgeris Matthaios , Guery Benoit , Ianculescu Nicoleta , Dunet Vincent , Messaoudi Yosra , Pistocchi Silvia , Tozzi Piergiorgio , Kirsch Matthias , Monney Pierre 
Published: March 2023

Abstract

Background
Cerebral embolic events (CEEs) are common complications of infective endocarditis (IE), and their presence can modify diagnosis and therapeutic plans. The aim of the present study was to assess the role of cerebral imaging (Cer-Im) on diagnosis and management of patients with suspected IE.

Methods
This study was conducted at the Lausanne University Hospital, Lausanne, Switzerland, from January 2014 to June 2022. CEEs and IE were defined according to modified Duke criteria of the European Society of Cardiology (ESC) guidelines.

Results
Among 573 patients with IE suspicion and Cer-Im, 239 (42%) patients had neurological symptoms. At least 1 CEE was found in 254 (44%) episodes. Based on Cer-Im findings, episodes were reclassified from rejected to possible or from possible to definite IE in 3 (1%) and 25 (4%) patients, respectively (0% and 2% in asymptomatic patients, respectively). Among the 330 patients with possible or definite IE, at least 1 CEE was found in 187 (57%) episodes. A new surgical indication (in association with left-side vegetation &gt;10 mm) was established in 74/330 (22%) IE patients and 30/155 (19%) asymptomatic IE patients, respectively.

Conclusions
Cer-Im in asymptomatic patients with IE suspicion showed limited potential for improving the diagnosis of IE. In contrast, performing Cer-Im in asymptomatic patients with IE may be useful for decision making, because Cer-Im findings led to the establishment of new operative indication for valvular surgery in one fifth of patients according to ESC guidelines.
Resource and seasonality drive interspecific variability in simulations from a dynamic energy budget model
Authors: Mariño Joany , Dufour Suzanne C , Hurford Amy , Récapet Charlotte 
Published: March 2023

AbstractAnimals show a vast array of phenotypic traits in time and space. Such variation patterns have traditionally been described as ecogeographical rules; for example, the tendency of size and clutch size to increase with latitude (Bergmann's and Lack's rules, respectively). Despite considerable research into these variation patterns and their consequences for biodiversity and conservation, the processes behind trait variation remain controversial. Here, we show how food variability, largely set by climate and weather, can drive interspecific trait variation by determining individual energy input and allocation trade-offs. Using a dynamic energy budget (DEB) model, we simulated different food environments, as well as interspecific variability in the parameters for energy assimilation, mobilization and allocation to soma. We found that interspecific variability is greater when the resource is non-limiting in both constant and seasonal environments. Our findings further show that individuals can reach larger biomass and greater reproductive output in a seasonal environment than in a constant environment of equal average resource due to the peaks of food surplus. Our results agree with the classical patterns of interspecific trait variation and provide a mechanistic understanding that supports recent hypotheses which explain them: the resource and the eNPP (net primary production during the growing season) rules. Due to the current alterations to ecosystems and communities, disentangling the mechanisms of trait variation is increasingly important to understand and predict biodiversity dynamics under climate change, as well as to improve conservation strategies.
Identification of volatile compounds from fruits aroma and citrus essential oils and their effect on oviposition of Ceratitis capitata (Diptera: Tephritidae)
Authors: Antonatos Spyridon , Anastasaki Eirini , Balayiannis George , Michaelakis Antonios , Magiatis Prokopios , Milonas Panagiotis , Papadopoulos Nikolaos T , Papachristos Dimitrios P 
Published: March 2023

Abstract
Ceratitis capitata (Wiedemann), also known as the Mediterranean fruit fly, is one of the most serious pests for several fresh fruit commodities causing extensive fruit losses worldwide. The response of C. capitata adults to fruit and nonfruit volatiles has been extensively studied. However, the linkage between fruit volatiles and the female ovipositional choice has not been fully elucidated. The present study focused on identifying the volatile organic compounds emitted by detached intact fresh fruits (oranges, lemons, bergamots, and apples) and citrus essential oils and evaluate their effect on Mediterranean fruit fly oviposition. There were more than 130 and 45 volatiles compounds detected in fruits odors and citrus essential oils, respectively. The volatile profile of fruits was dominated either from terpenes and terpenoids or from esters of butanoic, hexanoic, and octanoic acids while limonene was by far the most abundant compound in all citrus essential oils. Oviposition of C. capitata was strongly affected by volatiles emanated from both intact fruit and the citrus essential oils. Regarding the volatiles of the intact fruits, the odor of sweet orange elicited strong oviposition responses to females, while bergamot had the least stimulatory effect on oviposition. Bergamot oil also elicited the least oviposition stimulation compared to sweet orange and lemon essential oils. Our discussion elaborates on the role of fruit volatiles on host finding behavior and fruit susceptibility to C. capitata infestation and includes possible practical implication of the above findings.
A step to the left? Gender ideologies and political party identification in Germany
Authors: Diabaté Sabine , Grunow Daniela , Braack Mirko 
Published: March 2023

AbstractAfter decades in which gender relations, as anchored in work-family policies and egalitarian gender ideologies, moved towards greater equality, the status quo is currently being challenged. Gender-ambivalent ideologies have spread in both the political and private realms. It is unclear how the rise in gender-ambivalence documented in current research relates to party identification. On the one hand, gender ambivalence may represent a variation of post-materialist liberal values corresponding with support for centre-left parties in Germany. On the other, ambivalence may reflect a modified form of traditionalism and thus, a step to the left among voters leaning towards right-wing and conservative parties. This paper uses the Leitbild Survey 2012 to provide empirical evidence to fill this research gap. In line with cross-national research, latent class analyses reveal four main gender ideologies among young Germans: unidimensional ‘egalitarian’ and ‘moderate traditional’, as well as ambivalent, multidimensional ‘secondary earner’, and ‘intensive parenting’. Multinomial regression models show that egalitarian class members identify with centre-left parties while members of the ambivalent, multidimensional secondary earner, and intensive parenting classes as well as those of the moderate traditional class identify more strongly with centre-right parties. In light of the broader literature on gender ideology change, which documents a steep decline in traditionalism, our cross-sectional findings may be interpreted as capturing a step to the left among voters leaning towards right-wing and conservative parties in Germany, who now accept new mothers combining care-giving and part-time-work.