bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automation in flow cytometry: Guidelines and review of systems
Authors: Al‐Attar Ahmad , Kumar Kirthi R. , Untersee Dorian , O'Driscoll Marci , Ventura Miguel Francoise S. , Lin Leo 
Published: May 2023

AbstractAutomation in flow cytometry has recently advanced from the partial laboratory automation and robotics islets, to more fully integrated systems. This article reviews three manufacturers' newest sample preparation systems: the Beckman CellMek, the Sysmex PS‐10, and the BD FACSDuet. These three instruments are capable of performing many of the manual steps in flow cytometry sample processing (pipetting, staining, lysing, washing, fixing). General description, capabilities, advantages, and disadvantages of each system are compared. Overall, these systems have the potential to become mainstay items in today's busy clinical flow cytometry laboratories, and save a significant amount of hands‐on time for laboratory staff.
Adaptive plasticity and fitness costs of endangered, nonendangered, and invasive plants in response to variation in nitrogen and phosphorus availabilities
Authors: Minden Vanessa , Verhoeven Koen , Olde Venterink Harry 
Published: May 2023

AbstractGlobal change drivers such as eutrophication and plant invasions will create novel environments for many plant species. Through adaptive trait plasticity plants may maintain their performance under these novel conditions and may outcompete those showing low‐adaptive trait plasticity. In a greenhouse study, we determined if plasticity in traits is adaptive or maladaptive in endangered, nonendangered, and invasive plant species in response to variation of nitrogen (N) and phosphorus (P) availability (N:P ratios 1.7, 15, and 135) and whether plastic trait responses are adaptive and/or costly for fitness (i.e., biomass). Species choice comprised 17 species from three functional groups (legumes, nonlegume forbs, and grasses), either classified as endangered, nonendangered, or invasive. After 2 months, plants were harvested and nine traits related to carbon assimilation and nutrient uptake were measured (leaf area, SLA, LDMC, SPAD, RMR, root length, SRL, root surface area, and PME activity). We found more traits responding plastically to variation in P than in N. Plasticity only created costs when P was varied. Plasticity in traits was mostly adaptively neutral toward fitness, with plasticity in three traits being similarly adaptive across all species groups: SPAD (as a measure of chlorophyll content, adaptive to N and P limitation), leaf area, and root surface area (adaptive to P limitation). We found little differences in trait plasticity between endangered, nonendangered, and invasive species. Synthesis. Along a gradient from N limitation, balanced N:P supply, and P limitation, we found that the type of fluctuating nutrient (i.e., if N or P is varied) is decisive for the adaptive value of a trait. Variation in P availability (from balanced supply to P limitation) created both a stronger reduction in fitness as well as created plasticity costs in more traits than variation in N availability (from balanced supply to N limitation). However, the patterns observed in our study may change if nutrient availability is altered, either by nutrient inputs or by a shift in nutrient availabilities, for example, by decreasing N input as foreseen by European Legislation, but without simultaneously decreasing P input.
Establishing environmental DNA and RNA protocols for the simultaneous detection of fish viruses from seawater
Authors: Ip Yin Cheong Aden , Chen Jing , Tan Li Ying , Lau Clara , Chan Ying Hui , Shanmugavelu Balasubramaniam Ravendrakumar , Wong Wan Yen Jovinc , Ng Kaitlyn , Tan Zi Yan Brian , Fernandez Charlene Judith , Chang Siow Foong , Yap Him Hoo 
Published: May 2023

AbstractAquatic viruses are major threats to global aquacultural productivity. While conventional diagnostic methods for disease investigation are laborious, time‐consuming, and often involve the sacrifice of animals, environmental DNA and RNA (eDNA/eRNA) tools have the potential in being non‐invasive alternatives for the effective and early detection of various pathogens simultaneously. In this study, three seawater filtration methods—Sterivex syringe filtration, centrifugal ultrafiltration, and vacuum pump filtration with iron flocculation—were assessed for the recovery rates in co‐detecting fish virus eDNA/eRNA from natural seawater that was spiked with fish red seabream iridovirus (RSIV, DNA virus) and nervous necrosis virus (NNV, RNA virus). The centrifugal ultrafiltration method was the most effective for the capture of small‐sized viruses like NNV with a recovery rate of 63.23%, while the method of vacuum pump filtration with iron flocculation and chloroform disintegration of filter membranes had the highest RSIV recovery rate of 32.61%. We also optimized both automated and manual nucleic acid extraction methods and found comparable eDNA/eRNA extraction efficiencies. Our findings from the systematic comparison of seawater filtration and extraction methods suggest that each seawater filtration/nucleic acid extraction method can cater to different aquatic animal virus surveillance and disease investigation scenarios. These highlight the potential of virus eDNA/eRNA approaches for advancing the field of disease ecology and safeguarding aquatic animal health.
Exopolysaccharide from Lacticaseibacillus rhamnosus induces IgA production in airways and alleviates allergic airway inflammation in mouse model
Authors: Srutkova Dagmar , Kozakova Hana , Novotna Tereza , Gorska Sabina , Hermanova Petra Petr , Hudcovic Tomas , Svabova Tereza , Sinkora Marek , Schwarzer Martin 
Published: May 2023

AbstractThe currently observed high prevalence of allergic diseases has been associated with changes in microbial exposure in industrialized countries. Defined bacterial components represent a new strategy for modulating the allergic immune response. We show that intranasal administration of exopolysaccharide (EPS) isolated from Lacticaseibacillus (L.) rhamnosus LOCK900 induces TGF‐β1, IgA, and regulatory FoxP3+ T‐cells in the lungs of naïve mice. Using the ovalbumin mouse model, we demonstrate that intranasal administration of EPS downregulates the development of allergic airway inflammation and the Th2 cytokine response in sensitized individuals. At the same time, EPS treatment of sensitized mice, similar to EPS‐induced responses in naïve mice, significantly increased the level of total, OVA‐specific, and also bacteria‐specific IgA in bronchoalveolar lavage and the number of IgA‐producing B‐cells in the lung tissue of these mice. Thus, EPS derived from L. rhamnosus LOCK900 can be considered a safe candidate for preventing the development of allergic symptoms in the lungs of sensitized individuals upon exposure to an allergen.
Characterizing the Volatile Profile and Flavor of Four Kinds of Hotpot Seasonings in China Using HS‐GC‐MS, HS‐GC‐IMS, and Sensory Evaluation
Authors: Han Jiajia , Fu Chenggang , Wan Yilai , Guo Jiahao , Wei Changqing , Liu Wenyu 
Published: May 2023

AbstractHotpot is a distinctive dish of East Asian cuisine, especially in China. Herein, headspace‐gas chromatography‐mass spectrometry, headspace‐gas chromatography‐ion mobility spectrometry, and sensory evaluation are applied to compare the volatile organic compounds (VOCs) of four types of hotpot seasoning oils (HSOs) and establish their characteristic fingerprints. A total of 203 VOCs are detected by gas chromatography‐mass spectrometry (GC‐MS). Among them, linalool, anethole, and estragole contribute to the overall aroma of all four HSOs (relative odor activity value ≥ 0.1). Gas chromatography‐ion mobility spectrometry (GC‐IMS) results reveal that 77 VOCs are identified, comprising 19 terpenoids, 18 aldehydes, 14 alcohols, 13 esters, 6 ketones, 3 acids, 2 furans, 1 pyrazine, and 1 sulfide. Meanwhile, principal component analysis demonstrates that GC‐IMS is an important tool for the rapid classification of hotpot seasonings. Finally, the sensory evaluation team concludes that the horse fat hotpot seasoning has promising development prospects. This study validates the applicability of GC‐IMS and GC‐MS for distinguishing VOCs in hotpots, and the findings provide a theoretical basis for flavor differentiation in HSOs.Practical Applications: Horse oil is significantly lower in cholesterol than butter, and thus possesses potential benefits for human health. This study provides a basis to produce horse oil hotpots and a reference for identifying the VOCs in HSOs.
Kdo Glycosylations and Their Application in Oligosaccharide Synthesis**
Authors: Kumar Pradhan Tapan 
Published: May 2023

AbstractHigher carbon saccharide 3‐deoxy‐d‐manno‐oct‐2‐ulosonic acid (Kdo) is a structural unit of bacterial lipopolysaccharides (LPSs) and capsular polysaccharides (CPSs). Kdo is present in the inner core region of LPSs, and this region is structurally conserved. Being non‐mammalian in origin, Kdos are effectively recognized by the native and adaptive immune systems. Therefore, the synthesis of new Kdo derivatives and neoglycoconjugates is highly important for the development of vaccines. This review highlights recent accomplishments related to α‐glycosylations, β‐glycosylations and C‐glycosylations of Kdos and their application to the stereoselective synthesis of inner core oligosaccharides.
Recent progress on nature‐derived biomaterials for eco‐friendly triboelectric nanogenerators
Authors: Song Kyo Yong , Kim Sun‐Woo , Nguyen Dinh Cong , Park Ji Young , Luu Thien Trung , Choi Dukhyun , Baik Jeong Min , An Seongpil 
Published: May 2023

AbstractEco‐friendly triboelectric nanogenerator (eco‐TENG) is considered as a next‐generation renewable kinetic energy‐harvesting technology, especially for its potential use as a power supply unit for self‐powered electronics. For eco‐TENGs, nature‐derived biomaterials, which are non‐toxic to human and environment, highly biocompatible, and abundant in nature, are used for tribopositive or tribonegative materials, or both. Here, recent progress in nature‐derived biomaterials exploited for eco‐TENGs and their energy‐harvesting performances are reviewed, focusing on refined, hybridized, post‐treated, or not. Since biomaterials that exist in nature have evolved over hundreds of years to hundreds of thousands of years, their diversity, novelty in physicochemical structure, and high safety to nature hold enormous potential as carbon‐neutral energy materials. Furthermore, scalability and cost‐effectiveness of their fabrication methods make them promising as industrially viable eco‐materials.image
Decay of ignimbrite fairy chimneys of Arizona's Basin and Range Province, USA
Authors: Dorn Ronald I. 
Published: May 2023

AbstractFairy chimneys of ignimbrite are well studied in Cappadocia in central Anatolia, Turkey. The ignimbrite landforms of Arizona's Basin and Range, USA, in contrast, remain relatively unexplored in geomorphic scholarship, despite decades of geological research on the characteristics of the ignimbrites themselves. This research focuses on the rock‐decay processes that modify Arizona's Basin and Range fair chimneys. Dissolution of glass and groundmass, measured along joints using digital image processing of back‐scattered electron microscope imagery, reveal a 3× higher porosity than inside the rock interior. This method also measured a 3‐4× increase in dissolution near the base of the chimney compared with the rock interior, leading to notch development that promotes chimney mass wasting and subsequent ballistic impacts. Epilithic organisms studied with electron microscopy both enhance rock decay and promote case hardening. These observations are consistent with prior understanding of how fairy chimneys evolve over time: columnar joints exposed on cliff faces experience enough decay to allow erosion of joint material, this erosion separates the chimney from the rock face and notch development near the chimney base promotes mass wasting. The rock‐decay processes studied here likely operate on very different timescales. Mass wasting events leading to ballistic impacts occur at observable timescales. Varnish microlamination dating, using ultrathin sections of rock varnish formed on fairy chimneys, indicates that the case hardening of the top of fairy chimneys are much more stable than the sides; the tops last experienced surface detachment during the early Holocene, as opposed to the sides that experienced flaking throughout the late Holocene. The timescale of basal notch development is not known, with the exception that the very surface of notches erode at rates too fast to accumulate rock varnish. The timescales of epilithic organisms enhancing rock decay and dissolution of columnar joints are not known.
Nickel Oxide Decorated Halloysite Nanotubes as Sulfur Host Materials for Lithium–Sulfur Batteries
Authors: Elibol Meltem Karaismailoglu , Jiang Lihong , Xie Dongjiu , Cao Sijia , Pan Xuefeng , Härk Eneli , Lu Yan 
Published: May 2023

AbstractLithium–sulfur batteries with high energy density still confront many challenges, such as polysulfide dissolution, the large volume change of sulfur, and fast capacity fading in long‐term cycling. Herein, a naturally abundant clay material, halloysite, is introduced as a sulfur host material in the cathode of Li–S batteries. Nickel oxide nanoparticles are embedded into the halloysite nanotubes (NiO@Halloysite) by hydrothermal and calcination treatment to improve the affinity of halloysite nanotubes to polysulfides. The NiO@Halloysite composite loaded with sulfur (S/NiO@Halloysite) is employed as the cathode of Li–S batteries, which combines the physical confinements of tubular halloysite particles and good chemical adsorption ability of NiO. The S/NiO@Halloysite electrode exhibits a high discharge capacity of 1205.47 mAh g−1 at 0.1 C. In addition, it demonstrates enhanced cycling stability, retaining ≈60% of initial capacity after 450 cycles at 0.5 C. The synthesized NiO@Halloysite can provide a promising prospect and valuable insight into applying natural clay materials in Li–S batteries.
Discourses of work life in narrative identities of people with advanced dementia
Authors: Kłosińska Urszula , Ziółkowska Justyna 
Published: May 2023

AbstractObjectivesThere is no consensus as to whether people with advanced dementia can create a narrative identity. It is most often thought to be disturbed due to autobiographical memory disorders. In this paper, we examined how people with advanced dementia constructed their narrative identities in relation to their professional experience.MethodsThis qualitative study used data obtained from 8 semi‐structured interviews. The interviewees were people with advanced dementia aged between 66 and 89 years. We analyzed the dataset based on the textual‐oriented discourse analysis.ResultsThe study participants created narrative identities. Their narrative identities were constructed within residual professional discourses learned during their lifespan. These discourses blended their narrative identities into coherent stories about who they are now, offered languages to describe current experiences, and emphasized important values for their self‐image. The participants built narrative identities by referring to the past and imagining a better present with the omission of the future. The past was valued positively and was a source of positive nostalgia. Projections of a better present served to reveal their needs and assess ways to meet them.ConclusionsWe argue that people with advanced dementia can create complex and coherent narrative identities. They are constructed around discourses and not only using autobiographical memories. Encouraging them to create narrative identities in the dialogue can be a simple therapeutic method by which they can maintain a sense of self‐cohesion and belonging to the world.
The predictive usefulness of the novel “combined hemoglobin‐to‐platelet ratio and maximum mouth opening index” on prevalence of radiation induced trismus in patients with nasopharyngeal cancer
Authors: Somay Efsun , Yilmaz Busra , Topkan Erkan , Kucuk Ahmet , Pehlivan Berrin , Selek Ugur 
Published: May 2023

AbstractObjectivesTo explore how well a unique combination of hemoglobin‐to‐platelet ratio (HPR) and pretreatment maximum mouth opening (MMO) predicts the prevalence of radiation‐induced trismus (RIT).Patients and methodsHPR and MMO cutoff values (0.54 and 40.7 mm) divided patients into two groups. To develop the novel HPR‐MMO index, four combinations of these factors were tested for predictive power: Group 1: HPR > 0.54 and MMO > 40.7 mm; Group 2: HPR ≤ 0.54 but MMO > 40.7 mm; Group 3: HPR > 0.54 but MMO ≤ 40.7 mm; Group 4: HPR ≤ 0.54 and MMO ≤ 40.7 mm.ResultsData of 198 patients with LA‐NPC was analyzed retrospectively. RIT rates for Groups 1 to 4 were 10.2%, 15.2%, 25%, and 59.4%. Groups 2 and 3 were merged to generate HPR‐MMO index because of statistically equivalent RIT rates: Low‐risk: HPR > 0.54 and MMO > 40.7 mm; Intermediate‐risk: HPR ≤ 0.54 but MMO > 40.7 mm or; HPR > 0.54 but MMO ≤ 40.7 mm; High‐risk: HPR ≤ 0.54 and MMO ≤ 40.7 mm. It was revealed that the low‐, high‐, and intermediate‐risk group's RIT rates; 10.2%, 59.4%, and 19.2%, respectively.ConclusionThe novel HPR‐MMO index may to classify LA‐NPC patients into low, intermediate, and high‐risk RIT groups.
Binational confidential enquiry of maternal deaths due to postpartum hemorrhage in France and the Netherlands: Lessons learned through the perspective of a different context of care
Authors: de Vries P. L. M. , van den Akker T. , Bloemenkamp K. W. M. , Grossetti E. , Rigouzzo A. , Saucedo M. , Verspyck E. , Zwart J. , Deneux‐Tharaux C. 
Published: May 2023

AbstractObjectiveTo learn lessons for maternity care by scrutinizing postpartum hemorrhage management (PPH) in cases of PPH‐related maternal deaths in France and the Netherlands.MethodsIn this binational Confidential Enquiry into Maternal Deaths (CEMD), 14 PPH‐related maternal deaths were reviewed by six experts from the French and Dutch national maternal death review committees regarding cause and preventability of death, clinical care and healthcare organization. Improvable care factors and lessons learned were identified. CEMD practices and PPH guidelines in France and the Netherlands were compared in the process.ResultsFor France, new insights were primarily related to organization of healthcare, with lessons learned focusing on medical leadership and implementation of (surgical) checklists. For the Netherlands, insights were mainly related to clinical care, emphasizing hemostatic surgery earlier in the course of PPH and reducing the third stage of labor by prompter manual removal of the placenta. Experts recommended extending PPH guidelines with specific guidance for women refusing blood products and systematic evaluation of risk factors. The quality of CEMD was presumed to benefit from enhanced case finding, also through non‐obstetric sources, and electronic reporting of maternal deaths to reduce the administrative burden.ConclusionA binational CEMD revealed opportunities for improvement of care beyond lessons learned at the national level.
Serum CA125 as a biomarker for dysmenorrhea in adenomyosis
Authors: Tang Ying , Wen Ming‐bo , Xiang Ru‐mei , Yang Ming‐tao , Shu Bin , Xu Fan , Li Jun , Hu Hui‐quan , Shi Qiuling 
Published: May 2023

AbstractObjectiveTo explore the association between CA125 and dysmenorrhea in adenomyosis, and the factors affecting CA125 in adenomyosis.MethodsPatients were grouped a the presence of dysmenorrhea. The receiver operating characteristic (ROC) curve was applied to assess the utility of CA125 for dysmenorrhea. Binary logistic regression was employed to identify the factors associating dysmenorrhea and CA125 level.ResultsPatients in the dysmenorrhea group had higher CA125 levels than those in the non‐dysmenorrhea group. For those with dysmenorrhea, CA125 levels of diffuse subtype group were higher than those of the focal subtype group. The CA125 level of 35 U/mL was validated as the optimal cut‐point for dysmenorrhea in ROC curves. Compared with patients whose CA125 was 35 U/mL or less, those with CA125 levels greater than 35 U/mL were more likely to have dysmenorrhea. Thereafter, the multiple regression analysis showed that adenomyotic lesion volume was positively correlated with CA125 level in the total cohort and subtype groups, while age was negatively correlated with CA125 level in the total cohort and diffuse subtype.ConclusionsThe pathogenesis of adenomyosis is not clear. CA125 was associated with dysmenorrhea in adenomyosis, and, furthermore, CA125 level is positively correlated with the severity of the disease.
Research progress of CRISPR/Cas systems in nucleic acid detection of infectious diseases
Authors: Dong Jinying , Du Yuguang , Zhou Lei 
Published: May 2023

AbstractInfectious diseases are a serious threat to human health, and accurate, rapid and convenient early detection of pathogens is the first step of active treatment. Technologies that detect pathogens have advanced significantly because of the development of fundamental disciplines and the integration of multidisciplinary fields. Among these technologies, nucleic acid detection technology is preferred because of its rapid measurement, accuracy and high sensitivity. The CRISPR/Cas system, consisting of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR‐associated (Cas), is an adaptive immune system that specifically recognizes, binds and cleaves exogenous invasive nucleic acids. The CRISPR/Cas system is widely found in bacteria and archaea. Researchers have developed nucleic acid detection technologies with single‐molecule sensitivity, single‐base precision specificity, portability and low cost based on the specific cleavage and trans‐cleavage activities of the CRISPR/Cas system. The next generation of in‐vitro diagnostics is shifting to nucleic acid technology because this technology shows promise in a wide range of applications in resource‐constrained environments. In this review, the development and mechanism of the CRISPR/Cas system are presented together with representative CRISPR/Cas applications in nucleic acid detection. Additionally, the review summarizes future perspectives and trends of the CRISPR/Cas system in nucleic acid detection.
The role of the proteasome in mitochondrial protein quality control
Authors: Rödl Saskia , Herrmann Johannes M. 
Published: May 2023

AbstractThe abundance of each cellular protein is dynamically adjusted to the prevailing metabolic and stress conditions by modulation of their synthesis and degradation rates. The proteasome represents the major machinery for the degradation of proteins in eukaryotic cells. How the ubiquitin‐proteasome system (UPS) controls protein levels and removes superfluous and damaged proteins from the cytosol and the nucleus is well characterized. However, recent studies showed that the proteasome also plays a crucial role in mitochondrial protein quality control. This mitochondria‐associated degradation (MAD) thereby acts on two layers: first, the proteasome removes mature, functionally compromised or mis‐localized proteins from the mitochondrial surface; and second, the proteasome cleanses the mitochondrial import pore of import intermediates of nascent proteins that are stalled during translocation. In this review, we provide an overview about the components and their specific functions that facilitate proteasomal degradation of mitochondrial proteins in the yeast Saccharomyces cerevisiae. Thereby we explain how the proteasome, in conjunction with a set of intramitochondrial proteases, maintains mitochondrial protein homeostasis and dynamically adapts the levels of mitochondrial proteins to specific conditions.