bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Squid Species Using DNA Barcoding and Real-time PCR
Authors: Gao Hongwei , Li Rui , Sun Min , Yue Zhiqin , Zhang Hongwei 
Published: July 2023

Abstract
Squid is an important economic aquatic product that is widely consumed worldwide. Because of their special taste, some squid species are sold at a higher price. And with allergen proteins in squid, some foods need to show the raw material information of allergens from Ommastrephidae-derived materials. For this reason, several practical, sensitive, and specific techniques have been proposed aimed at detecting adulterations. Here, we performed DNA barcode and real-time PCR methods to identify species and Ommastrephidae-derived materials on squids and sea foods. In this study, DNA was extracted from different sea foods and processed foods. The DNA barcode assay specifically targets 16S rRNA, LSU, and COI genes to identify species origin. A real-time PCR method using the 12S rRNA gene sequence was tested to detect Ommastrephidae-derived materials in deeply processed foods. The results show that primers for 16S rRNA and COI genes can detect all samples from three subfamilies of species not only in Ommastrephidae but also in Cephalopoda, Bivalvia, and Gastropoda species with good robustness. The LODs of real-time PCR were 48.80, 3.05, and 12.02 pg for Illicinae, Ommastrephinae, and Todarodinae, respectively. For samples with high Ct values greater than 30, the DNA template of the real-time PCR should be below 781 pg to avoid pseudo-positives caused by Loliginidae-derived materials. The developed DNA-based method can be used for squid species and Ommastrephidae-derived materials in squid products as industry standards.
The Impact of Preoperative Biliary Drainage on Postoperative Healthcare-Associated Infections and Clinical Outcomes Following Pancreaticoduodenectomy: A Ten-Year Retrospective Analysis
Authors: Yu Zheng-Hao , Du Ming-Mei , Zhang Xuan , Suo Ji-Jiang , Xie Xiao-Lian , Xiao Wei , Zeng Tao , Lu Qing-Bin , Liu Yun-Xi , Yao Hong-Wu 
Published: July 2023

Abstract
Background
Pancreaticoduodenectomy (PD) is a complex procedure and easily accompanied by healthcare-associated infections (HAIs). This study aimed to assess the impact of PBD on postoperative infections and clinical outcomes in PD patients.
Methods
The retrospective cohort study were conducted in a tertiary hospital from January 2013 to December 2022. Clinical and epidemiological data were collected from HAIs surveillance system and analyzed.
Results
Among 2842 patients who underwent PD, 247 (8.7%) were diagnosed with HAIs, with surgical site infection being the most frequent type (n = 177, 71.7%). A total of 369 pathogenic strains were detected, with Klebsiella pneumoniae having the highest proportion, followed by Enterococcu and Escherichia coli. Although no significant association were observed generally between PBD and postoperative HAIs, subgroup analysis revealed that PBD was associated with postoperative HAIs in patients undergoing robotic PD (aRR = 2.174; 95% CI:1.011 − 4.674; P = 0.047). Prolonging the interval between PBD and PD could reduce postoperative HAIs in patients with cholangiocarcinoma (≥ 4 week: aRR = 0.292, 95% CI 0.100 − 0.853; P = 0.024) and robotic PD (≤ 2week: aRR = 3.058, 95% CI 1.178 − 7.940; P = 0.022). PBD was also found to increase transfer of patients to ICU (aRR = 1.351; 95% CI 1.119 − 1.632; P = 0.002), extended length of stay (P < 0.001) and postoperative length of stay (P = 0.004).
Conclusion
PBD does not exhibit a significant association with postoperative HAIs or other outcomes. However, the implementation of robotic PD, along with a suitable extension of the interval between PBD and PD, appear to confer advantages concerning patients' physiological recuperation. These observations suggest potential strategies that may contribute to enhanced patient outcomes.
Effect of rGO/Au PMMA nanocomposite thin film thickness on photodetector devices
Authors: Ahmed Abdullah Khalid , Sabbar Ehsan H. , Al-Hiti Ahmed Shakir 
Published: July 2023

Abstract

Nanotechnology is a new expanding field of research including manipulating characteristics and nanoscale structures. Nanoparticles (NPs) have recently received a lot of attention in various applications such as biotechnological, fiber laser, optical sensors, etc. Here, we present a novel synthesis approach using reduced graphene oxide (rGO)/gold (Au) nano and embedding them into Polymethyl methacrylate (PMMA) using the laser-induced forward transfer (LIFT) technique to form three samples of different thicknesses. These samples were measured by atomic force microscopy (AFM) to be 200 nm, 230 nm, and 240 nm, respectively. The energy bandgap, Ultraviolet-visible (UV-Vis) spectrum, Scanning Electron Microscopy (SEM) image, and photodetector were used for the characterization of the samples. The highest specific quantum efficiency of 200 nm, 230, and 240 nm thin film samples was 141.5%, 171.86%, and 173%, at 890 nm; while directivity was realized at 1.15 × 1013 Jones, 1.7 × 1013 Jones, and 1.80 × 1013 Jones at 890 nm, respectively. The LIFT films improved responsivity (Rλ), directivity (D*), quantum efficiency (η%), and Current-Voltage (I-V) measurements.
The evaluation of wood and its products based on the classification of wood and added value
Authors: Chen Songyang , Yang Tiantian , He Xin , Cao Xiulong , Zhu PeiQi , Qiu Jian 
Published: July 2023

Abstract
This paper examines the calculation system that combines the basic value and additional value of wood raw materials in order to develop a calculation model for the value evaluation of wood and wood products. It then arrives at the value evaluation scheme that is determined by the classification of the wood. The classification of wood raw materials into protected wood, unprotected wood, and wood products is based on the extent of plant protection and timber value classification. The value adjustment coefficient determined by the classification level is investigated in accordance with the existing national law. We categorize the evaluation system into three groups based on the hierarchical processing approach: Standing wood, logs, and sawn timber are valued according to Scheme 1, wood goods containing protected information are valued according to Scheme 2, and wood products without protected information are valued according to Scheme 3. The wood value predicted by this model largely follows the rule of value of historical examples after the application of many forest damage occurrences. When applying the evaluation method of this scheme in batches as opposed to the way of determining the value of a single piece, the evaluation findings are more systematic, which significantly boosts the effectiveness of inspection and value evaluation of wood and its products.
Measurement and Modelling of Excess Molar Volume and Excess Enthalpy of n-tridecane or n-tetradecanewithdecalin By Application of PFP Theory
Authors: Touazi Ahmed Amin , Boussaadia Abd El Nour , Didaoui Saeda , Nasrallah Noureddine , Chelghoum Fetah , Benziane Mokhtar 
Published: July 2023

Abstract
The experimental measurement of density and enthalpy of mixture for two binary liquid mixtures of n-tridecane or n-tetradecane with decalin was reported in this paper. The measurements were conducted at a temperature range of 293.15 to 323.15K and at 303.15K using calorimeter C80. The mixtures were analyzed at various proportions, including the entire composition range and infinitely diluted. The excess molar volume (VE) and excess molar enthalpy (HE) of mixtures were calculated and fitted using the Redlich-Kister equation. The paper observed the expansion phenomenon for the VE at all temperatures, including over the entire composition range and infinitely diluted. Additionally, the HE exhibited endothermic behavior at the studied temperature range and composition range. The Prigogine-Flory-Patterson (PFP) theory was utilized to predict both thermodynamic properties, namely the VE and HE. The results obtained using the PFP theory were compared with those obtained using the Treszczanowicz and Benson association (TB) model for VE and with the NRTL, Wilson, and Flory models for HE. The PFP model, which employed a single fitted parameter to describe VE, demonstrated satisfactory performance in predicting VE. Conversely, the Treszczanowicz and Benson association (TB) model yielded relatively poor results in fitting VE. However, the NRTL, Wilson, PFP, and Flory models exhibited good performance in predicting HE.
Clinical Outcomes After Gastrostomy Tube Removal in Children: Risk Factors for Complications &amp; Growth Outcome
Authors: Nguyen Rebecca , Kennedy Alison , Varajavandi Vincent , Krishnan Usha 
Published: July 2023

Abstract
Purpose
To determine the incidence of and risk factors for complications after gastrostomy tube removal in children and to investigate whether complications are associated with growth outcome.
Methodology:
Medical records of 108 patients, who underwent gastrostomy removal and gastrostomy site closure, between January 2014 to July 2022 at a single institution, were reviewed. Clinical factors were analysed for association with complications and growth outcomes.
Results
Complications occurred in 39% (42/108) of patients. Persistent gastrocutaneous fistula (GCF) (20/108, 18%) and perioperative complications (16/82, 20%) were most frequent. Attempted non-operative closure (NOC), PPI use, and steroid use were more frequent in patients with complications (64.3% vs 28.8%, p = 0.001; 57.3% vs 32.3%, p = 0.029; 29.3% vs 12.5%, p = 0.033 respectively). Patients with persistent GCF had longer mean duration of gastrostomy placement (40.45 ± 35.36 vs 15.96 ± 16.94 months, p=0.008) and greater occurrence of pre-existing non-reflux gastrointestinal disease (40% vs 12%, p=0.038). Mean growth z-scores were maintained at 6 months, 18 months and 5 years follow-up in patients with and without complications.
Conclusion
Attempted NOC, PPIs, and steroids were risk factors for complications after gastrostomy removal. Longer duration of gastrostomy placement and non-reflux gastrointestinal disease were risk factors for persistent GCF. Complications were not associated with poorer growth outcome.
Biosensors as early warning detection systems for waterborne Cryptosporidium
Authors: Siwak Andrea M. , Baker Priscilla G. , Dube Admire 
Published: July 2023

Abstract

Waterborne disease is a global health threat contributing to a high burden of diarrhoeal disease, and growing evidence indicates a prospective increase in incidence coinciding with the profound effects of climate change. A major causative agent of gastrointestinal disease is Cryptosporidium, a protozoan waterborne parasite identified in over 70 countries. Cryptosporidium is a cause of high disease morbidity in children and the immunocompromised with limited treatment options for patients at risk of severe illness. The hardy nature of the organism leads to its persistence in various water sources, with certain water treatment procedures proving inefficient for its complete removal. While diagnostic methods for Cryptosporidium are well-defined in the clinical sphere, detection of Cryptosporidium in water sources remains suboptimal due to low dispersion of organisms in large sample volumes, lengthy processing times and high costs of equipment and reagents. A need for improvement exists to identify the organism as an emerging threat in domestic water systems, and the technological advantages that biosensors offer over current analytical methods may provide a preventative approach to outbreaks of Cryptosporidium. Biosensors are innovative, versatile and adaptable analytical tools that could provide highly sensitive, rapid, on-site analysis needed for Cryptosporidium detection in low-resource settings.
Spatiomolecular Characterization of Dopamine D2 Receptors Cells in the
Mouse External Globus Pallidus
Authors: Espallergues Julie , Boubaker-Vitre Jihane , Mignon Audrey , Avrillon Maelle , Bon-Jego Morgane Le , Baufreton Jerome , Valjent Emmanuel 
Published: July 2023

Abstract:
The external globus pallidus (GPe) is part of the basal ganglia circuit and plays a key role in
controlling the actions. Although, many evidence indicate that dopamine through its activation of dopamine
D2 receptors (D2Rs) modulates the GPe neuronal activity, the precise spatiomolecular characterization
of cell populations expressing D2Rs in the mouse GPe is still lacking. By combining single
molecule in situ hybridization, cell type-specific imaging analyses, and electrophysiology slice recordings,
we found that GPe D2R cells are neurons preferentially localized in the caudal portion of GPe.
These neurons comprising pallido-striatal, pallido-nigral, and pallido-cortical neurons segregate into
two distinct populations displaying molecular and electrophysiological features of GPe GABAergic
PV/NKX2.1 and cholinergic neurons respectively. By clarifying the spatial molecular identity of GPe
D2R neurons in the mouse, this work provides the basis for future studies aiming at disentangling the
action of dopamine within the GPe.
Amalgamated Pharmacoinformatics Study to Investigate the Mechanism of Xiao Jianzhong Tang against Chronic Atrophic Gastritis
Authors: Lian Xu , Fan Kaidi , Qin Xuemei , Liu Yuetao 
Published: July 2023

Background:Traditional Chinese medicine (TCM) Xiaojianzhong Tang (XJZ) has a favorable efficacy in the treatment of chronic atrophic gastritis (CAG). However, its pharmacological mechanism has not been fully explained.Objective:The purpose of this study was to find the potential mechanism of XJZ in the treatment of CAG using pharmacocoinformatics approaches.Methods:Network pharmacology was used to screen out the key compounds and key targets, MODELLER and GNNRefine were used to repair and refine proteins, Autodock vina was employed to perform molecular docking, Δ Lin_F9XGB was used to score the docking results, and Gromacs was used to perform molecular dynamics simulations (MD).Results:Kaempferol, licochalcone A, and naringenin, were obtained as key compounds, while AKT1, MAPK1, MAPK14, RELA, STAT1, and STAT3 were acquired as key targets. Among docking results, 12 complexes scored greater than five. They were run for 50ns MD. The free binding energy of AKT1-licochalcone A and MAPK1-licochalcone A was less than -15 kcal/mol and AKT1-naringenin and STAT3-licochalcone A was less than -9 kcal/mol. These complexes were crucial in XJZ treating CAG.Conclusion:Our findings suggest that licochalcone A could act on AKT1, MAPK1, and STAT3, and naringenin could act on AKT1 to play the potential therapeutic effect on CAG. The work also provides a powerful approach to interpreting the complex mechanism of TCM through the amalgamation of network pharmacology, deep learning-based protein refinement, molecular docking, machine learning-based binding affinity estimation, MD simulations, and MM-PBSA-based estimation of binding free energy.
Systems Pharmacology Strategy for the Investigation of Action Mechanisms
of Qin Herb Libanotis Buchtormensis (Fisch.) DC. in Bone Diseases
Authors: Feng Rundong , Wang Lifang , Chai Hu , Jiao Jie , Zhang Peng , Zheng Xu , Liu Haijing , Zhang Wenjuan , Wu Suli 
Published: July 2023

Introduction:
Qin medicines are medicinal plants growing in habitat around the peak of Qinling Mountain. Their unique curative effects on bone metabolic diseases and pain diseases have been favoured by the local people in clinical trials for thousands of years. Libanotis buchtormensis (Fisch.) DC. (LBD), is one of the popular Qin herbs, which has been widely used for the treatment of various diseases, such as osteoporosis, rheumatic, and cardiovascular diseases. However, due to the multiple compounds in LBD, the underlying molecular mechanisms of LBD remain unclear.

Objective:
This study aimed to systemically investigate the underlying mechanisms of LBD against bone diseases.

Methods:
In this study, a systems pharmacology platform included the potential active compound screening, target fishing, and network pharmacological analysis was employed to decipher the ac-tion mechanisms of LBD.

Results:
As a result, 12 potential active compounds and 108 targets were obtained. Furthermore, compound-target network and target-pathway network analysis showed that multi-components in-teracted with multi-targets and multi-pathways, i.e., MARK signalling pathway, mTORC1 signal-ling pathway, etc., involved in the regulation of the immune system and circulatory system. These results suggested the mechanisms of the therapeutic effects of LBD on various diseases through most compounds targeted by multiple targets.

Conclusion:
In conclusion, we successfully predicted the LBD bioactive compounds and potential targets, implying that LBD could be applied as a novel therapeutic herb in osteoporosis, rheumatic, and cardiovascular diseases. This work provides insight into the therapeutic mechanisms of LBD for treating various diseases.
An Artificial Therapist (Manage Your Life Online) to Support the Mental Health of Youth: Co-Design and Case Series
Authors: Wrightson-Hester Aimee-Rose , Anderson Georgia , Dunstan Joel , McEvoy Peter M , Sutton Christopher J , Myers Bronwyn , Egan Sarah , Tai Sara , Johnston-Hollitt Melanie , Chen Wai , Gedeon Tom , Mansell Warren 
Published: July 2023

Background
The prevalence of child and adolescent mental health issues is increasing faster than the number of services available, leading to a shortfall. Mental health chatbots are a highly scalable method to address this gap. Manage Your Life Online (MYLO) is an artificially intelligent chatbot that emulates the method of levels therapy. Method of levels is a therapy that uses curious questioning to support the sustained awareness and exploration of current problems.

Objective
This study aimed to assess the feasibility and acceptability of a co-designed interface for MYLO in young people aged 16 to 24 years with mental health problems.

Methods
An iterative co-design phase occurred over 4 months, in which feedback was elicited from a group of young people (n=7) with lived experiences of mental health issues. This resulted in the development of a progressive web application version of MYLO that could be used on mobile phones. We conducted a case series to assess the feasibility and acceptability of MYLO in 13 young people over 2 weeks. During this time, the participants tested MYLO and completed surveys including clinical outcomes and acceptability measures. We then conducted focus groups and interviews and used thematic analysis to obtain feedback on MYLO and identify recommendations for further improvements.

Results
Most participants were positive about their experience of using MYLO and would recommend MYLO to others. The participants enjoyed the simplicity of the interface, found it easy to use, and rated it as acceptable using the System Usability Scale. Inspection of the use data found evidence that MYLO can learn and adapt its questioning in response to user input. We found a large effect size for the decrease in participants’ problem-related distress and a medium effect size for the increase in their self-reported tendency to resolve goal conflicts (the proposed mechanism of change) in the testing phase. Some patients also experienced a reliable change in their clinical outcome measures over the 2 weeks.

Conclusions
We established the feasibility and acceptability of MYLO. The initial outcomes suggest that MYLO has the potential to support the mental health of young people and help them resolve their own problems. We aim to establish whether the use of MYLO leads to a meaningful reduction in participants’ symptoms of depression and anxiety and whether these are maintained over time by conducting a randomized controlled evaluation trial.
Adherence to treatment in young people with perinatal HIV infection in the first years after transition from pediatric to the adult medical service
Authors: Safonova P. V. , Koltsova O. V. 
Published: July 2023

Objective. To assess ART adherence and treatment continuity in young adults living with HIV since birth or childhood in the first years after transition from paediatric to adult service of the AIDS Center, as well as to analyse factors associated with treatment adherence interruption after transition to adult care.Materials and methods. In our study, 86 cases of young people living with HIV since birth or childhood were obtained by chart audit and analyzed, among whom 53.5% were girls and 64.0% lived at home at the time of their 18th birthday, who have been transferred from paediatric to adult care of the AIDS Center for more than one year.Results and discussion. The majority (92%) of young adults living with HIV since birth or childhood retained in the health care system in the first year after the transition, but only 53–62% were highly adherent to treatment and monitoring for HIV infection in the first years after the transition. Episodes of non-adherence to treatment during childhood and adolescence, «more complex» ART scheme and the lack of adult’s social support at the time of 18th birthday increase the likelihood of interruption in treatment adherence in the first year after transition to adult care, while the combination of these factors has negative consequences for adherence and continuity of treatment for a longer period of time.Conclusion. Successful transition from paediatric to adult care for patients living with HIV since birth or childhood requires special additional attention of the health care system, contributes to the health protection of each patient, aims to stop HIV in the second generation.
The global burden of HIV/AIDS in Russia in terms of public health. Part 1
Authors: Zagdyn Z. M. , Kobesov N. V. , Verbitskaya E. V. , Denuyshenkov V. L. 
Published: July 2023

Objective. Evaluation of the global HIV burden in Russia, identification of progress made and outstanding problems for 1987–2021.Materials and methods. The analysis of national and foreign publications, data obtained from Rosstat, from forms of federal statistical observation and the FNM of the AIDS Center of Rospotrebnadzor was carried out. The study used information-analytical, epidemiological and statistical methods. Mathematical calculations were performed using the SPSS-26 program.Results and discussion. In Russia, the target indicators of the state program of HIV/AIDS response strategy is being provided in different directions: the greatest success has been achieved in conducting chemoprevention of mother to child HIV transmission, somewhat less in the coverage of the population with HIV testing, which amounted to 26.1% versus the target indicator of 30.0% in 2021, the number of newly detected cases of HIV infection is decreasing (from 73.7 to 71.0 thousand people in 2020–2021, versus the target indicators of 76.1 and 72.3 thousand people, respectively), there is decreasing the HIV incidence (40.4 per 100 thousand. in 2021 versus 68.5 in 2015). Antiretroviral therapy (ART) coverage of patients registered in AIDS Centers went up from 12.4% to 78.9% in 2007–2021. Meanwhile, the determining factor in the HIV/AIDS epidemic progress in Russia is a heterosexual route (F=75.1, p<0.0001) leading to the penetration of infection into all population segments. In addition, despite the increase in the coverage of ART for people living with HIV (PLWH), the HIV mortality rate is remaining high (11.6 per 100 thousand population in 2021, versus 7.9 in 2007).Conclusion. In recent years, there has been a positive trend in the coverage of three-stage HIV chemoprophylaxis in pregnant women, women in labor and newborns. The number of HIV population based screening tests is increasing annually. There is an increase in the ART coverage of patients and a decrease in the number of newly diagnosed cases of the disease. However, along with the observed increase in the HIV spread through heterosexual contacts, it is necessary to strengthen information and preventive measures, especially among the reproductive age group. The high HIV mortality rate is continuing to remain, despite the increase in ART coverage of PLWH. The identified problems need to be solved comprehensively based on the results of large-scale sociological research conducted in various regions of Russia.