bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaction of Caveolin-1 gene polymorphism with processed meats on anthropometric measurements and body composition in obese and overweight women: A cross-sectional study
Authors: Mohamadi Azam , Mirzababaei Atieh , Abaj Faezeh , Roumi Zahra , Khorraminezhad Leila , Mirzaei Khadijeh 
Published: July 2023

Abstract
Background: It has been shown in different studies that Caveolin (CAV-1) gene variants can play a role in causing obesity and changes in anthropometric measurements and body composition. Further, the consumption of processed meats can also cause obesity and changes in the anthropometric parameters. Therefore, this study sought to investigate the relationship between the interaction of processed meats and the CAV-1 gene polymorphism on anthropometric parameters and body composition.
Methods: This cross-sectional study was conducted on 389 obese and overweight women with a body mass index (BMI) 25-30 kg/m2 and 18-48 years from 20 health centers in Tehran. Dietary assessment was done using a 147-question semi-quantitative food frequency questionnaire (FFQ). Furthermore, anthropometric parameters, body composition, and biochemical assessments were measured. The CAV-1 genotyping was performed by the polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) method.
Results: It was observed that (AA+AG) group was younger than GG group (P-value<0/05). The mean ± SD of systolic blood pressure (SBP), diastolic blood pressure (DBP) waist hip ratio (WHR), and waist circumference (WC) were 111.68 ±13.71 (mmHg) 77.53 ±10.44 (mmHg), 1.16 ±4.61 and 99.12 ± 9.41 (cm), respectively. Our findings also showed, after controlling for age, BMI, physical activity and energy intake, significant interactions were found between processed meat and the A allele carrier group on WC (p = 0.04), body mass index (BMI ) (p = 0.02), body fat mass (BFM) (p = 0.01), body roundness index (BRI) (p = 0.006) and fat mass index (FMI) (p = 0.01). Moreover, in interaction, WC, WHR, visceral fat level (VFL) and a body shape index (ABSI) had higher level compared to GG homozygotes but these differences were not significant (P>0.05).
Conclusion: The results of the present study showed that high consumption of processed meats may be associated with higher anthropometric parameters and body composition in AA and AG genotypes with rs-3807992. These results showed that diet, gene type and their interaction should be considered in the evaluation of anthropometric measurements and body composition.
Post-SARS-CoV-2 vaccine small vessel vasculitis in a 13-year-old girl: A case report
Authors: Hajiani Shabnam , Johari Leila , Moshfeghinia Reza , Alyasin Soheila Sadat , Nabavizadeh Seyed Hesamedin , Esmaeilzadeh Hossein 
Published: July 2023

Abstract
Background
Cutaneous small vessel vasculitis (CSVV) or leukocytoclastic vasculitis (LCV) is a subgroup of vasculitis localized to the skin. Regarding the COVID-19 vaccine, it is postulated that some patients develop an inflammatory reaction to vaccine components encoding the SARS-CoV‐2 spike glycoprotein, which targets the endothelium and results in CSVV with endothelial cell inflammation and dysfunction. Although post-COVID-19 vaccination small vessel vasculitis has been reported in the literature and some cases had necrotic reactions after receiving Pfizer and AstraZeneca.
Case presentation:
We report the first pediatric case of post-Sinopharm COVID-19 vaccine small vessel vasculitis. Five months after receiving the second dose of the mentioned vaccine, a 13-year-old girl developed gangrene-like symptoms, starting as painful, tingling sensations on the left forefinger. Spiral CT angiography of both upper extremities showed vasculitis and narrowing of the left radial and ulnar arteries. Pathology specimen from a punch biopsy of the skin was consistent with acute neutrophilic LCV involving small blood vessels, and the bone marrow aspiration biopsy noted a moderately hypocellular marrow. Our treatment included a single dose of cyclophosphamide, 2 doses of rituximab, 5 episodes of plasmapheresis (QOD), vasodilators, and sympathetic blockade. A good outcome was achieved.
Conclusions
Post-COVID-19 small vessel vasculitis in pediatric patients is rare but can be diagnosed and treated effectively using histopathological analysis and specific treatments.
The clinical significance of inflammatory biomarkers, IL-6 cytokine, and systemic immune inflammatory index in rabbit model of acute and chronic Methicillin-resistant Staphylococcus epidermidis-induced osteomyelitis
Authors: Ancuta Diana-Larisa , Lovati Arianna Barbara , Coman Cristin 
Published: July 2023

Abstract
Infections are a major complication of open fractures and fracture fixation. In this study, an innovative bioactive medical device was used to experimentally treat MRSE-induced osteomyelitis in rabbit tibia. This paper investigates the clinical significance of inflammatory biomarkers (NLR, PLR, MLR and PMR), SII and IL-6 and assesses their role in the development of osteomyelitis. The main objective is to identify the utility of hematological reports derived from neu-trophils, leukocytes, monocytes and platelets in the evolution of implant-related osteomyelitis and the estimation of treatment efficiency. In particular, this study compares the response of these inflammatory markers to different treatments in the presence or absence of bioactive materials and/or topical antibiotics over time. The analysis of the threads showed that NLR, PLR and SII had high values in the acute phase of the disease, so that after chronicization, they decrease. The animals treated with vancomycin nano-functionalized peptide-enriched silk fibroin-coated implants showed lower levels of inflammatory biomarkers compared to the other groups (empty implants and peptide-enriched silk fibroin-coated implants). NLR, PLR and SII, complemented by IL-6 can be used as fairly accurate biomarkers for the diagnosis of osteomyelitis.
The Impact of Perioperative Enhanced Recovery Nursing Model on Postoperative Delirium and Rehabilitation Quality in Elderly Patients with Femoral Neck Fractures
Authors: Wang Cheng , Tan Bingyin , Qian Qing 
Published: July 2023

Abstract
Background
The aim of this study was to investigate the effects of introducing the Enhanced Recovery After Surgery (ERAS) nursing model on postoperative delirium occurrence and rehabilitation quality in elderly patients with femoral neck fractures.
Methods
A total of 160 elderly patients with femoral neck fractures, who met the inclusion criteria and were admitted between March 2021 to March 2023, were divided into two groups: the traditional care group and the ERAS nursing model group. In addition to traditional care measures, the ERAS nursing model group received interventions based on the principles of the ERAS nursing model. The occurrence of delirium and sleep quality were observed at 24, 48, and 72 hours postoperatively, as well as during the overall hospital stay. The duration of hospitalization, time to first mobilization, and post-discharge follow-up on quality of life were compared between the two groups.
Results
The ERAS nursing model group exhibited a significant difference in the occurrence of delirium at 48 and 72 hours postoperatively, as well as during the overall hospital stay (P < 0.05). However, there was no significant difference in the occurrence of delirium at 24 hours postoperatively (P > 0.05). The sleep quality of the two groups showed a statistically significant difference (P < 0.05). The ERAS nursing model group had shorter time to first mobilization, reduced hospitalization duration, and higher Harris and SF-36 scores during post-discharge follow-up, compared to the traditional care group (P < 0.05).
Conclusions
The implementation of the ERAS nursing model in elderly patients with femoral neck fractures improved postoperative sleep quality, reduced delirium occurrence, shortened average hospitalization duration, and enhanced patients' quality of life.
Anticancer Medicinal Plants Used by the Natives in the Elgon Sub-region, Eastern Uganda
Authors: Kudamba Ali , Kasolo Josephine N , Bbosa Godfery S , Lugaajju Allan , Wabinga Henry , Niyonzima Nixon , Ocan Moses , Damani Ali M , Kafeero Hussein M , Walusansa Abdul , Ssenku Jamilu E , Alemu Shaban O , Lubowa Muhammad , Muwonge Haruna 
Published: July 2023

Abstract
Introduction: Medicinal plants have been used to treat several diseases, including cancer in Uganda. However, very few studies have been reported on the anticancer medicinal plants used in the Elgon sub-region. The main aim of this study was to describe the anticancer medicinal plants used to treat cancer, with an emphasis on prostate cancer, in the Elgon sub-region of Eastern Uganda.
Methods: Information was gathered using a semi-structured, researcher-administered questionnaire, interview guides, and guided field walks. Quantitative indices such as fidelity level (FL), preference ranking, and informant consensus factor (ICF) were evaluated. To summarize ethnobotanical data, a descriptive statistical analysis and graphs were created by SPSS version 21.0 and GraphPad Prism version 8.125, respectively, and narratives were explained using theories and major hypotheses in ethnobotany.
Results: A total of 50 plant species from 36 families were described; of them, 45% were herbs and 10% were parasites. Additionally, 60% of plants were gathered from forest reserves, and 86% of the materials used by herbalists were leaves. The most effective treatment for cancer overall was Tylosema fassoglensis due to its versatility. Hydnora abyssinica A. Br., Hydnora africana, Rhoicissus tridentata (L.f.) Wild & R.B. Drumm., Prunus africana, and Kigelia africana, respectively, are used to cure prostate cancer. Herbs make up 45% of all medical plant use, with the fidelity level (FL) for Hydnora abyssinica A. Br being 93.9%, Rhoicissus tridentata (L.f.) Wild & R.B. Drumm (89.7%), Hydnora africana (84.8%), and Prunus africana (82.4%). Herbalists predominantly used leaves (86%), and 60% of the plants used as medicines were gathered from peripheral forest areas, according to their preferred ranking. The most popular methods of preparation and administration (86%) were oral routes and concoctions. Hydnora abyssinica A. Br. was the herb that was most sought after for treating prostate cancer. ICF values larger than 0.5 were only attained by plant species used to treat cervical and prostate cancer, indicating that herbalists often lack awareness of herbs used in cancer treatment.
Conclusions and recommendation: All the first ten frequently used plants in the treatment of cancer received faithfulness levels more than 50%, suggesting that herbalists have faith in the ability of these plants to treat cancer. Therefore, it is crucial to conduct additional research into their phytochemistry, toxicity, efficacy, and effectively enforced conservation.
Evaluation of Salivary Anti-CCP in Relation to Some Oral Manifestations in Rheumatoid Arthritis Patients, Sulaimaniyah, Iraq
Authors: Fadhil Hevi Nihad Mohammed , Ahmed Khadija Mohammed 
Published: July 2023

Abstract
Background
Rheumatoid arthritis (RA) affects the oral cavity, mainly the exocrine salivary gland function causing decreased salivary flow rate.
Objectives
To determine the salivary flow rate, pH, and the main oral manifestations in RA patients. Also, to evaluate the level of antibodies to the cyclic citrullinated peptide (CCP) in the saliva.
Subjects and methods
A matched case-control study was conducted at Rheumatology Centre, Sulaimaniyah, Iraq, on 90 subjects (50 RA patients and 40 healthy controls) from January 2021 to January 2022. The oral cavity was thoroughly examined for signs of oral and temporomandibular joint (TMJ) involvement and to assess the Decayed, Missed, Filled Teeth (DMFT) index and oral health status. Saliva samples were taken from each subject to measure flow rate, pH, and immunological determination of anti-CCP antibodies using ELISA.
Results
Salivary flow rate was substantially reduced in RA patients. The main oral manifestations were oral ulcerations, redness of the tongue, dry/cracked lips, buccal mucosal dryness, taste disturbances, angular cheilitis, and candidiasis. In addition, TMJ involvement and DMFT were also suggestively more in the RA patients. There was no significant difference between both groups regarding oral hygiene index and the level of salivary anti-CCP antibodies.
Conclusions
Patients with RA exhibited decreased salivary flow rate and generally poorer oral health status.
Lung function impairment may increase secondary thrombocytopenia susceptibility: a Mendelian randomization
Authors: Liu Yuxin , Bin Chengli , Ran Qiang , Liu Yuan , Zhang Jieying , Liu YanKun 
Published: July 2023

Abstract
Purpose
This study explored the relationship between lung function and secondary thrombocytopenia by analyzing Mendelian randomization (MR).
Methods
As instrumental genetic variables, appropriate single nucleotide polymorphisms (SNPs) were extracted from genome-wide association study (GWAS) data on lung function. We choose secondary thrombocytopenia as the outcome. Inverse variance weighting, weighted median methods, and MR- Egger's method were used to explore the association between lung function and the risk of secondary thrombocytopenia. The analysis results were shown as odds ratio (OR) and 95% confidence interval to evaluate the causal association between lung function parameters and secondary thrombocytopenia.
Results
The inverse variance weighted (IVW) analysis showed that forced expiratory volume in the first second (FEV1) significantly led to the occurrence of secondary thrombocytopenia [OR: 0.2126; 95% confidence interval (CI): 0.0508–0.8886, P = 0.0339], reduced peak expiratory flow (PEF) increased the occurrence of secondary thrombocytopenia [OR: 0.1018; 95% CI: 0.0143–0.7234, P = 0.0224]. The decline of FEV1 and PEF contributed to the risk of secondary thrombocytopenia. Furthermore, FVC (Forced Vital Capacity), FEV1/FVC, and lung volume were not associated with secondary thrombocytopenia.
Conclusion
FEV1 and PEF could be important precipitating factors of secondary thrombocytopenia, which provided new ideas for treating and preventing secondary thrombocytopenia.
Protocol of a Parallel, Randomized Controlled Trial on the Effects of a Novel Personalized Nutrition Approach by Artificial Intelligence in Real World Scenario
Authors: Feng Jingyuan , Liu Hongwei , Mai Shupeng , Su Jin , Sun Jing , Zhou Jianjie , Zhang Yingyao , Wang Yinyi , Wu Fan , Zheng Guangyong , Zhu Zhenni 
Published: July 2023

AbstractBackground Nutrition service needs are huge in China. Previous studies indicated that personalized nutrition (PN) interventions were effective. The aim of the present study is to identify the effectiveness and feasibility of a novel PN approach supported by artificial intelligence (AI). Methods This study is a two-arm parallel, randomized, controlled trial in real world scenario. The participants will be enrolled among who consume lunch at a staff canteen. In Phase I, a total of 170 eligible participants will be assigned to either intervention or control group on 1:1 ratio. The intervention group will be instructed to use the smartphone applet to record their lunches and reach the real-time AI-based information of dish nutrition evaluation and PN evaluation after meal consumption for 3 months. The control group will receive no nutrition information but be asked to record their lunches though the applet. Dietary pattern, body weight or blood pressure optimizing is expected after the intervention. In phase II, the applet will be free to all the diners (about 800) at the study canteen for another one year. Who use the applet at least 2 days per week will be regarded as the intervention group while the others will be the control group. Body metabolism normalization is expected after this period. Generalized linear mixed models will be used to identify the dietary, anthropometric and metabolic changes. Discussion This novel approach will provide real-time AI-based dish nutrition evaluation and PN evaluation after meal consumption in order to assist users with nutrition information to make wise food choice. This study is designed under a real-life scenario which facilitates translating the trial intervention into real-world practice. Trial registration This trial has been registered with the Chinese Clinical Trial Registry (ChiCTR2100051771; date registered: 03/10/2021).
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering
Authors: Haas Heinrich , Graewert Melissa , Wilhelmy Christoph , Bacic Tijana , Schumacher Jens , Blanchet Clement , Meier Florian , Drexel Roland , Welz Roland , Kolb Bastian , Bartels Kim , Nawroth Thomas , Klein Thorsten , Svergun Dmitri , Langguth Peter 
Published: July 2023

Abstract
We present a generically applicable approach to determine an extensive set of size-dependent critical quality attributes inside nanoparticulate pharmaceutical products. By coupling asymmetrical-flow field-flow fractionation (AF4) measurements directly in-line with solution small angle X-ray scattering (SAXS), vital information such as (i) quantitative, absolute size distribution profiles, (ii) drug loading, (iii) size-dependent internal structures, and (iv) quantitative information on free drug is obtained. Here the validity of the method was demonstrated by characterizing complex mRNA-based lipid nanoparticle products. The approach is particularly applicable to particles in the size range of 100 nm and below, which is highly relevant for pharmaceutical products – both biologics and nanoparticles. The method can be applied as well in other fields, including structural biology and environmental sciences.
Schnurri-3 inhibition rescues skeletal fragility and vascular skeletal stem cell niche pathology in a mouse model of osteogenesis imperfecta
Authors: Xu Ren , Li Na , Shi Baohong , Li Zan , Han Jie , Sun Jun , Yallowitz Alisha , Bok Seoyeon , Xiao Shuang , Wu Zouxing , Chen Yu , Xu Yan , Qin Tian , Lin Zhiming , Zheng Haiping , Shen Rong , Greenblatt Matthew 
Published: July 2023

Abstract

Osteogenesis imperfecta (OI) is a disorder of low bone mass and increased fracture risk due to a range of genetic variants that prominently include mutations in genes encoding type Ⅰ collagen. While it is well known that OI reflects defects in the activity of bone-forming osteoblasts, it is currently unclear whether OI also reflects defects in the many other cell types comprising bone, including defects in skeletal vascular endothelium or the skeletal stem cell populations that give rise to osteoblasts and whether correcting these broader defects could have therapeutic utility. Here, we find that numbers of skeletal stem cells (SSCs) and skeletal arterial endothelial cells (AECs) are augmented in Col1a2oim/oim mice, a well-studied animal model of moderate to severe OI, suggesting that disruption of a vascular SSC niche is a feature of OI pathogenesis. Moreover, crossing Col1a2oim/oim mice to mice lacking a negative regulator of skeletal angiogenesis and bone formation, Schnurri 3 (SHN3), not only corrected the SSC and AEC phenotypes but moreover robustly corrected the bone mass and spontaneous fracture phenotypes. As this finding suggested a strong therapeutic utility of SHN3 inhibition for the treatment of OI, a bone-targeting AAV was used to mediate Shn3 knockdown, rescuing the Col1a2oim/oim phenotype and providing therapeutic proof-of-concept for targeting SHN3 for the treatment of OI. Overall, this work both provides proof-of-concept for inhibition of the SHN3 pathway and more broadly addressing defects in the stem/osteoprogentior niche as is a strategy to treat OI.
Assessment of S. Aureus Growth on Biocompatible 3D Printed Materials: A Step Toward Tailored 3D Printed Laryngectomy Tubes
Authors: Senderovich Nicole , Shah Sharan , Ow Thomas J. , Rand Stephanie , Nosanchuk Joshua , Wake Nicole 
Published: July 2023

AbstractThe customizability of 3D printing allows for the manufacturing of personalized medical devices such as laryngectomy tubes, but it is vital to establish the biocompatibility of printing materials to ensure that they are safe and durable. The goal of this study was to assess the presence ofS. aureusbiofilms on a variety of 3D printed materials (two surgical guide resins, a photopolymer, an elastomer, and a thermoplastic elastomer filament) as compared to standard, commercially available laryngectomy tubes. C-shaped discs (15 mm in height, 20 mm in diameter, and 3 mm in thickness) were printed with five different biocompatible 3D printing materials andS. aureusgrowth was compared to Shiley™ laryngectomy tubes made from polyvinyl chloride. Discs of each material were inoculated withS. aureuscultures and incubated overnight. All materials were then removed from solution, washed in phosphate-buffered saline to remove planktonic bacteria, and sonicated to detach biofilms. Some solution from each disc was plated and colony-forming units were manually counted the following day. The resulting data was analyzed using a Kruskal-Wallis and Wilcoxon Rank Sum test to determine pairwise significance between the laryngectomy tube material and the 3D printed materials. The Shiley™ tube grew a median of 320 colonies (IQR 140–520), one surgical guide resin grew a median of 640 colonies (IQR 356–920), the photopolymer grew a median of 340 colonies (IQR 95.5–739), the other surgical guide resin grew a median of 431 colonies (IQR 266.5–735), the thermoplastic elastomer filament grew a median of 188 colonies (IQR 113.5–335), and the elastomer grew a median of 478 colonies (IQR 271–630). Using the Wilcoxon Rank Sum test, manual quantification showed a significant difference between biofilm formation only between the Shiley™ tube and a surgical guide resin (p = 0.018). This preliminary study demonstrates that bacterial colonization was comparable among most 3D printed materials as compared to the conventionally manufactured device. Continuation of this work with increased replicates will be necessary to determine which 3D printing materials optimally resist biofilm formation.
Proton Pump Inhibitors and Chronic Kidney Disease Risk: A Comparative Study with Histamine-2 Receptor Antagonists
Authors: Kweon Takhyeon , Kim Yerim , Lee Kyungju , Seo Won-Woo , Seo Seung In , Shin Woon Geon , Shin Dong Ho 
Published: July 2023

Abstract
This observational study explored the association between proton pump inhibitor (PPI) and histamine-2 receptor antagonist (H2RA) use and the risk of chronic kidney disease (CKD). Using the National Health Insurance Service-National Sample Cohort (NHIS-NSC) and six-hospital electronic health record (EHR) databases, CKD incidence was analyzed among PPI and H2RA users. Propensity score matching was used to balance baseline characteristics, with 1,858 subjects each in the PPI and H2RA groups from the NHIS-NSC, and 5,772 in EHR databases. CKD incidence was similar for both groups (7.39/1000 person-years vs. 7.03/1000 person-years; HR, 1.00; 95% CI, 0.56-1.79). A meta-analysis of the EHR databases showed no significant increased CKD risk associated with PPI use (HR = 1.01, 95% CI: 0.85-1.20). These results suggest PPI use may not increase CKD risk compared to H2RA use, but the potential role of PPI-induced CKD needs further research. Clinicians should consider this when prescribing long-term PPI therapy.