bluuebunny/crossref_metadata_2025_split huggingface dataset:
The sacroiliac dysfunction and pain is associated with history of lower extremity sport related injuries
Authors: Abdollahi Sajjad , Sheikhhoseini Rahman , Rahimi Mohammad , Huddleston Wendy E. 
Published: March 2023

Abstract
Background
The purpose of this study was to examine the association of sacroiliac joint (SIJ) dysfunction and pain with overuse and acute lower limb and pelvic girdle injuries of Iranian basketball players.

Methods
In this cross-sectional study, basketball-related injury data were collected during 2019–2020 from 204 basketball players of the Iranian league using the online Information Retrospective Injury Questionnaire. A researcher then performed ten clinical tests to assess SIJ dysfunction and pain (five tests for dysfunction and five tests for pain). Data analysis was performed by logistic regression at the confidence interval of 95%.

Results
Within our sample (n = 204), injury rates were calculated across sub-groups of athletes that had only SIJ pain (n = 19), only SIJ dysfunction (n = 67), both SIJ pain and dysfunction (n = 15) or no SIJ complaints (n = 103). Across these groups, a total of 464 injuries were reported. SIJ pain group reported 80 injuries (17.2%), SIJ dysfunction group reported 210 injuries (45.2%), both SIJ pain and dysfunction group reported 58 injuries (12.5%, and the no SIJ pain or SIJ dysfunction group reported 116 injuries (25.0%). Participants with SIJ pain were more likely to report previous pelvic girdle injuries (overuse: odds ratio (OR): 0.017; 95% CI: 0.005–0.56; p < 0.001 and acute: OR: 0.197; 95%CI: 0.101–0.384; p < 0.001) and also lower limb injuries (overuse: OR: 0.179, 95%CI: 0.082–0.392, p < 0.001). Participants with SIJ dysfunction only were likely to report acute pelvic girdle injuries (OR: 0.165; 95%CI: 0.070–0.387; p < 0.001) and acute lower limb injuries (OR: 0.165; 95%CI: 0.030–0.184; p < 0.001).

Conclusion
The presence of SIJ dysfunction and pain is associated with a history of acute and overuse injuries in the pelvic girdle and lower limb. Thus, SIJ dysfunction and pain should be specifically evaluated and addressed when designing rehabilitation programs for sports-related injuries.
The versatile roles of testrapanins in cancer from intracellular signaling to cell–cell communication: cell membrane proteins without ligands
Authors: Zhou Zhihang , Yang Zihan , Zhou Li , Yang Mengsu , He Song 
Published: March 2023

AbstractThe tetraspanins (TSPANs) are a family of four-transmembrane proteins with 33 members in mammals. They are variably expressed on the cell surface, various intracellular organelles and vesicles in nearly all cell types. Different from the majority of cell membrane proteins, TSPANs do not have natural ligands. TSPANs typically organize laterally with other membrane proteins to form tetraspanin-enriched microdomains (TEMs) to influence cell adhesion, migration, invasion, survival and induce downstream signaling. Emerging evidence shows that TSPANs can regulate not only cancer cell growth, metastasis, stemness, drug resistance, but also biogenesis of extracellular vesicles (exosomes and migrasomes), and immunomicroenvironment. This review summarizes recent studies that have shown the versatile function of TSPANs in cancer development and progression, or the molecular mechanism of TSPANs. These findings support the potential of TSPANs as novel therapeutic targets against cancer.
A necessary and sufficient condition of blow-up for a nonlinear equation
Authors: Liu Miao , Tang Jiangang , Lai Shaoyong 
Published: March 2023

AbstractWe investigate a nonlinear equation with quadratic nonlinearities, including a nonlinear model in Silva and Freire (J. Differ. Equ. 320:371–398, 2022). Using the classical energy estimate methods, we give a necessary and sufficient condition of blow-up of solutions to nonlinear equations. We answer a problem pointed out by Silva and Freire (J. Differ. Equ. 320:371–398, 2022).
The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathy
Authors: Cheng Yan-jiao , Wang Miao , Wang Jia , Cui Zhao , Zhao Ming-hui 
Published: March 2023

Abstract
Background
Most primary membranous nephropathy (MN) is mediated by anti-phospholipase A2 receptor (PLA2R) antibodies. Recently, these antibodies have been revealed months to years before the disease's onset. Their production and pathogenicity need further investigation.

Methods
Anti-PLA2R antibodies were purified from plasma of eight healthy individuals, 12 patients with PLA2R-related MN and negative circulating antibody (Ab-), and 18 patients with positive anti-PLA2R antibodies (Ab +), using affinity column coupled with recombinant human PLA2R. The antigen specificity, antibody amount, titer, IgG subclass, and affinity were assessed by Western blot, immunofluorescence, ELISA, and surface plasmon resonance.

Results
The natural anti-PLA2R antibodies recognized the conformational structure of PLA2R which locates on the cell membrane of podocytes. The amount of natural IgG was 0.12 ± 0.04 g/L, which accounted for 0.80% of total IgG and was lower than that of patients (2.36%, P < 0.001). The titer of natural antibodies was lower than that of patients in Ab- and Ab + groups (1:16 vs. 1:43 vs. 1:274, P < 0.001). IgG2(45.1%) was predominant in natural antibodies, while IgG4 was predominant in Ab + group (45.7 vs. 25.0%, P < 0.001). IgG1 was increasing from natural antibodies to Ab- and Ab + groups. The affinity of natural antibodies was lower than that of patients (KD: 641.0 vs. 269.0 vs. 99.6 nM, P = 0.002). The antibody titer, affinity, and IgG4 percentage were associated with the severity of proteinuria and the stages of membranous lesion.

Conclusions
The natural anti-PLA2R antibodies exist in healthy plasma. The antibody titer, IgG subclass, and affinity may participate in the pathogenesis of anti-PLA2R antibodies.
Geospatial assessment of urban green space using multi-criteria decision analysis in Debre Markos City, Ethiopia
Authors: Anteneh Mihret Bizuye , Damte Desta Solomon , Abate Simeneh Gedefaw , Gedefaw Abebaw Andarge 
Published: March 2023

AbstractUrban green space (UGS) serves as a lung for city dwellers to breathe and is critical for fostering the sustainable growth of urban ecology and improving residents’ quality of life. Debre Markos City (DMC) lacks natural recreational areas such as lakes and artificial green spaces. A notable UGS study utilizing multiple contributing factors integrated with remote sensing and GIS has a significant advantage in making residents more comfortable. This study, therefore, aimed to assess the conventional methods (existing and proposed UGS) against remote sensing and GIS-based, and to identify, quantify and allocate the proper suitable site for UGS development.. Hence, ten UGS influencing factors were selected. Each criterion was analyzed with the use of its proper algorithm. Multicriteria decision-making (MCDM) analysis integrated with Analytical Hierarchy Process (AHP) method and Remote Sensing and GIS were applied. The weighted sum overlay analysis method of spatial analysis was implemented based on the criteria weight of each factor. A 90.6% and 0.8855 accuracy assessment and kappa coefficient were recorded for land use/land cover classification, respectively. The value of the consistency ratio was 0.043, which is < 1 and acceptable. Results revealed that 13.12% (2201 ha), 25.47% (4271.7 ha), 30.89% (5180.8 ha), 22.49% (3772.7 ha), and 8.2% (1345.8 ha) of the total area coverage had very high, highly, moderately, poorly and unsuitable for UGS development activity at DMC, respectively. In this study, a resident can access UGS with less than 1 km radius distance, closer to the national standard of Ethiopia (i.e. 500 m). However, in the existing UGS, a person is forced to walk or travel more than 8.4 km to get the service. In conclusion, remote sensing and GIS can make life easy to access UGS in the study area with minimum distance, cost, and a short period. It is recommended that policymakers, planners, and executive bodies in Ethiopia consider the future urban demand of dwellers with proper studies earlier to establish other urban infrastructures.
PD-L1+ macrophages are associated with favorable features in primary mediastinal (thymic) large B-cell lymphoma
Authors: Steiner Raphael E. , Parra Edwin R. , Vega Francisco , Feng Lei , Westin Jason R. , Neelapu Sattva S. , Strati Paolo , Green Michael R. , Flowers Christopher R. , Solis Luisa M. , Wistuba Ignacio I. , Ahmed Sairah , Nair Ranjit , Hagemeister Fredrick B. , Noorani Mansoor , Marques-Piubelli Mario L. 
Published: March 2023

AbstractPrimary mediastinal (thymic) large B-cell lymphoma (PMBCL) is a rare, aggressive subtype of non-Hodgkin lymphoma and has a complex inflammatory microenvironment. Although most patients can be cured with standard-of-care immunochemotherapy, patients who have disease relapse have an unfavorable prognosis. Pre-treatment prognostic biomarkers in PMBCL are needed. In this retrospective study, we analyzed the clinical features and outcomes of PMBCL patients and their association with immune cell subpopulations identified by multiplex immunofluorescence at initial diagnosis. Two different antibody panels were used to assess macrophages in tissue biopsy specimens collected before the initiation of induction therapy. Twelve PMBCL patients, including five patients who had disease relapse, were included in the analysis. At a median follow-up time of 32.2 months, the median progression-free and overall survival durations were not reached. Our findings suggest that a high density of PD-L1+ macrophages is associated with favorable features, such as early disease stage and the absence of B-symptoms, and indicate that a high percentage of PD-L1+ macrophages and high densities of CD30+PD-L1+ cells and CD30+ cells might be associated with a lower risk of relapse within 12 months of therapy initiation. Further studies are needed to develop a biomarker signature predictive of treatment response with therapeutic consequences for patients with newly diagnosed PMBCL.
Categorizing the geometry of animal diel movement patterns with examples from high-resolution barn owl tracking
Authors: Luisa Vissat Ludovica , Cain Shlomo , Toledo Sivan , Spiegel Orr , Getz Wayne M. 
Published: March 2023

AbstractBackgroundMovement is central to understanding the ecology of animals. The most robustly definable segments of an individual’s lifetime track are its diel activity routines (DARs). This robustness is due to fixed start and end points set by a 24-h clock that depends on the individual’s quotidian schedule. An analysis of day-to-day variation in the DARs of individuals, their comparisons among individuals, and the questions that can be asked, particularly in the context of lunar and annual cycles, depends on the relocation frequency and spatial accuracy of movement data. Here we present methods for categorizing the geometry of DARs for high frequency (seconds to minutes) movement data.MethodsOur method involves an initial categorization of DARs using data pooled across all individuals. We approached this categorization using a Ward clustering algorithm that employs four scalar “whole-path metrics” of trajectory geometry: 1. (distance between start and end points), 2. from start point, 3. , and 4. . We illustrate the general approach using reverse-GPS data obtained from 44 barn owls,Tyto alba, in north-eastern Israel. We conducted a principle components analysis (PCA) to obtain a factor, , that essentially captures the scale of movement. We then used a generalized linear mixed model with as the dependent variable to assess the effects of age and sex on movement.ResultsWe clustered 6230 individual DARs into 7 categories representing different shapes and scale of the owls nightly routines. Five categories based on size and elongation were classified as closed (i.e. returning to the same roost), one as partially open (returning to a nearby roost) and one as fully open (leaving for another region). Our PCA revealed that the DAR scale factor, , accounted for 86.5% of the existing variation. It also showed that captures the openness of the DAR and accounted for another 8.4% of the variation. We also constructed spatio-temporal distributions of DAR types for individuals and groups of individuals aggregated by age, sex, and seasonal quadrimester, as well as identify some idiosyncratic behavior of individuals within family groups in relation to location. Finally, we showed in two ways that DARs were significantly larger in young than adults and in males than females.ConclusionOur study offers a new method for using high-frequency movement data to classify animal diel movement routines. Insights into the types and distributions of the geometric shape and size of DARs in populations may well prove to be more invaluable for predicting the space-use response of individuals and populations to climate and land-use changes than other currently used movement track methods of analysis.
Understanding examinees’ item responses through cognitive modeling of response accuracy and response times
Authors: Embretson Susan 
Published: March 2023

AbstractUnderstanding the cognitive processes, skills and strategies that examinees use in testing is important for construct validity and score interpretability. Although response processes evidence has long been included as an important aspect of validity (i.e., Standards for Educational and Psychological Tests, 1999), relevant studies are often lacking, especially in large scale educational and psychological testing. An important method for studying response processes involves explanatory mathematical modeling of item responses and item response times from variables that represent sources of cognitive complexity. For many item types, examinees may differ in strategies applied to responding to items. Mixture class item response theory models can identify latent classes of examinees with different processes, skills and strategies based on their pattern of item responses. This study will illustrate the use of response times in conjunction with explanatory item response theory models and mixture models, to provide information relevant to test validity and, hence, to score interpretations.
Spectroscopic single-molecule localization microscopy: applications and prospective
Authors: Brenner Benjamin , Sun Cheng , Raymo Françisco M. , Zhang Hao F. 
Published: March 2023

AbstractSingle-molecule localization microscopy (SMLM) breaks the optical diffraction limit by numerically localizing sparse fluorescence emitters to achieve super-resolution imaging. Spectroscopic SMLM or sSMLM further allows simultaneous spectroscopy and super-resolution imaging of fluorescence molecules. Hence, sSMLM can extract spectral features with single-molecule sensitivity, higher precision, and higher multiplexity than traditional multicolor microscopy modalities. These new capabilities enabled advanced multiplexed and functional cellular imaging applications. While sSMLM suffers from reduced spatial precision compared to conventional SMLM due to splitting photons to form spatial and spectral images, several methods have been reported to mitigate these weaknesses through innovative optical design and image processing techniques. This review summarizes the recent progress in sSMLM, its applications, and our perspective on future work.
Graphical Abstract
Management of off‐track Hill‐Sachs lesions in anterior glenohumeral instability
Authors: Calvo Emilio , Delgado Cristina 
Published: March 2023

AbstractBone loss has been identified as a risk factor for recurrent shoulder dislocations or failure after soft tissue repair. Although the range for “critical” bone loss is yet to be determined, glenoid and humeral bone defects should not be regarded as independent problems, but the interaction between them during shoulder motion should be evaluated as suggested by the glenoid track concept. The glenoid track concept is now widely accepted and considered essential for making decisions about surgery. Soft‐tissue procedures usually work well in patients with on‐track Hill‐Sachs lesions but in off‐track lesions do not. In this situation additional procedures should be performed.Different surgical options have been described to address off‐track Hill‐Sachs lesions, most commonly remplissage, Latarjet or free bone block procedures. Coracoid graft and free bone grafts convert the off‐track Hill‐Sachs lesion into on‐track by lengthening the glenoid‐track, whereas remplissage fill‐in the humeral lesion so that it does not engage. In the setting of a Hill‐Sachs lesion with little or no glenoid bone loss, remplissage has demonstrated satisfactory outcomes with a low complications and recurrence rate. Favorable results have been reported with glenoid bone grafting when managing isolated Hill‐Sachs or bipolar lesions. Studies analyzing Latarjet and Eden‐Hybinette procedures show that both procedures are safe and effective in the management of anterior glenohumeral instability. Attention should be paid to those patients with large bone defects not amenable to be restored with an isolated Latarjet that may be better addressed with an Eden‐Hybinnete or adding a remplissage to the Latarjet procedure.
Comparative in vitro treatment of mesenchymal stromal cells with GDF‐5 and R57A induces chondrogenic differentiation while limiting chondrogenic hypertrophy
Authors: Weißenberger Manuel , Wagenbrenner Mike , Nickel Joachim , Ahlbrecht Rasmus , Blunk Torsten , Steinert Andre F. , Gilbert Fabian 
Published: March 2023

AbstractPurposeHypertrophic cartilage is an important characteristic of osteoarthritis and can often be found in patients suffering from osteoarthritis. Although the exact pathomechanism remains poorly understood, hypertrophic de‐differentiation of chondrocytes also poses a major challenge in the cell‐based repair of hyaline cartilage using mesenchymal stromal cells (MSCs). While different members of the transforming growth factor beta (TGF‐β) family have been shown to promote chondrogenesis in MSCs, the transition into a hypertrophic phenotype remains a problem. To further examine this topic we compared the effects of the transcription growth and differentiation factor 5 (GDF‐5) and the mutant R57A on in vitro chondrogenesis in MSCs.MethodsBone marrow‐derived MSCs (BMSCs) were placed in pellet culture and in‐cubated in chondrogenic differentiation medium containing R57A, GDF‐5 and TGF‐ß1 for 21 days. Chondrogenesis was examined histologically, immunohistochemically, through biochemical assays and by RT‐qPCR regarding the expression of chondrogenic marker genes.ResultsTreatment of BMSCs with R57A led to a dose dependent induction of chondrogenesis in BMSCs. Biochemical assays also showed an elevated glycosaminoglycan (GAG) content and expression of chondrogenic marker genes in corresponding pellets. While treatment with R57A led to superior chondrogenic differentiation compared to treatment with the GDF‐5 wild type and similar levels compared to incubation with TGF‐ß1, levels of chondrogenic hypertrophy were lower after induction with R57A and the GDF‐5 wild type.ConclusionsR57A is a stronger inducer of chondrogenesis in BMSCs than the GDF‐5 wild type while leading to lower levels of chondrogenic hypertrophy in comparison with TGF‐ß1.
Association of main meal quality index with the odds of metabolic syndrome in Iranian adults: a cross-sectional study
Authors: Mirrafiei Amin , Hasanzadeh Mohaddeseh , Sheikhhossein Fatemeh , Majdi¹ Maryam , Djafarian Kurosh , Shab-Bidar Sakineh 
Published: March 2023

AbstractBackgroundMetabolic syndrome (MetS) is a common global issue linked to the quality of one’s eating occasions. The current cross-sectional study evaluates the association between a novel index, the Main Meal Quality Index (MMQI), and MetS among Iranian adults.MethodsA total of 824 men and women were recruited, and a 24-hour dietary recall assessed the dietary intake of the participants. Lunch was selected as the main meal based on energy density. The MMQI score was calculated based on ten components of dietary intake, with a higher score indicating more adherence to the index, with the final scores ranging from 0 to 100 points. The associations were assessed using binary logistic regression.ResultsThe mean age was 42.2 years and the range of the calculated MMQI was 22 to 86 (mean in total participants: 56.62, mean in women: 56.82, mean in men: 55.64). The total prevalence of MetS in the sample was 34%. After adjustments for potential confounders, the participants at the top quartile of MMQI had a lower odds ratio for hypertriglyceridemia and low high-density lipoprotein (HDL) level, and a higher odds ratio for hypertension, hyperglycemia, abdominal obesity, and MetS. The sex-specific analysis also did not show any significant associations between adherence to MMQI and MetS and its components.ConclusionOverall, MMQI is not associated with MetS and its components in a sample of Iranian men and women. More research is needed to examine MMQI and its possible association with current health-related problems including MetS.
“We know that our voices are valued, and that people are actually going to listen”: co-producing an evaluation of a young people’s research advisory group
Authors: Brady Louca-Mai , Miller Jacqueline , McFarlane-Rose Eleri , Noor Jasmine , Noor Rhianne , Dahlmann-Noor Annegret 
Published: March 2023

Abstract

Background
Children and young people’s (CYP) involvement is an increasing priority in UK healthcare and in heath research, alongside recognition that involving CYP in research requires different considerations to involving adults. Underpinned by children’s rights and a co-production ethos this paper, co-authored with young evaluators, explores the learning from a co-produced evaluation of eyeYPAG, a young persons’ research advisory group (YPAG) for eye and vision research based at Moorfields Eye Hospital, London, UK.

Methods
A team of young evaluators, supported by the eyeYPAG facilitator, conducted focus groups and online surveys with YPAG members, their parents and carers, researchers, group facilitators and funders. Qualitative data was analysed using a collaborative reflexive thematic analysis approach. Quantitative data, limited by the small number of participants, was analysed in Excel and reported as descriptive data.

Results
CYP valued the social and creative aspects of the group as well as learning about research and developing skills and confidence. Learning was a two-way process, with both researchers and facilitators reflecting on how much they had learnt from working with the YPAG. All participants talked about the importance of impact, feeling that CYP are making a difference to research, as well as CYP’s right to be involved. Effective planning and facilitation were key to the success of the group, in relation to accessibility and the development and delivery of sessions both online and in-person. Resourcing and administration were key challenges to this, as was engaging researchers who were not already converted to the public involvement cause. As the nature of a YPAG is that it primarily focuses on advising researcher-led projects, co-production was identified as something that the group was ‘working towards’, including through this evaluation. Co-producing with CYP involves building up knowledge, confidence and acknowledging power dynamics.

Conclusions
Co-producing an evaluation enabled us to learn about the benefits and challenges of involving CYP in research, as well as how to involve them in the development of that evidence. An ethos of co-production and children’s rights helped to shift the balance of power and develop more engaging and inclusive ways of working.