bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of 2D culture on stemness-related transcriptome in shed microvesicles of mesenchymal stem cells
Authors: Fard Fatemeh Foroughi , Bakhshandeh Behnaz , Jahanafrooz Zohreh 
Published: April 2023

Abstract
Background Over the past years, the applications of mesenchymal stem cells (MSCs) grow rapidly in medicine as a promising option for regenerative medicine. So, increasing demands for MSCs and their derivations as cell-based drugs in the market are anticipated. One of the major challenges in MSC therapy is possible alterations in the stemness potential of MSCs during in vitro culture and proliferation. Microvesicles (MVs) reflect the transcriptional status of cells of origin. This study aimed to investigate the impact of 2D culture on stemness characteristics of MSCs by focusing on transcriptome in shed MVs.Methods and Results MVs were isolated from rat bone marrow MSCs using ultracentrifugation. Transmission electron microscopy and dynamic light scattering were applied to characterizing MVs. Then, some markers related to stemness and differentiation were evaluated in shed MVs during 10-day culture. Meanwhile the cell proliferation and apoptosis were assessed in cultured MSCs. Based on our results, not only MSCs-derived MVs preserved somewhat their stemness contents but also MSCs maintained proliferative during 10-day 2D culture.Conclusion Taken together, 2D culture had no significant effect on the biological state of MSCs which strengthened their applications in cell therapies. Further studies are needed to identify the protein component of MVs to give some benefits for biomimetic approaches.
Wheat powdery mildew resistance gene Pm13 from Aegilops longissima encodes a unique mixed lineage kinase domain-like protein
Authors: Liu Wenxuan , Li Huanhuan , Men Wenqiang , Ma Chao , Liu Qianwen , Dong Zhenjie , Tian Xiubin , Wang Chaoli , Liu Cheng , Gill Harsimardeep , Ma Pengtao , Zhang Zhibin , Liu Bao , Zhao Yue , Sehgal Sunish 
Published: April 2023

Abstract
Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat comprise rich sources of diversity for powdery mildew resistance. Here, we report map-based cloning of broad-spectrum powdery mildew resistance gene Pm13 from wild wheat species Aegilops longissima. Pm13 encodes a novel mixed lineage kinase domain-like (MLKL) protein which contains an N-terminal HeLo domain and a C-terminal authentic serine/threonine kinase (STK) domain, a unique domain architecture that has not been reported in plant disease-resistance genes. The resistance function of Pm13 was validated by loss-of-function mutants, gene silencing, transgenic assay, and allelic association analyses. Thus, Pm13 represents a special class of plant resistance genes revealing a novel aspect of host-pathogen interactions. Though introgressed into wheat ~30 years ago it was hardly exploited due to linkage drag. The new lines with small Ae. longissima Pm13 segment will lead to wide-scale deployment in wheat cultivars.
Resistance to clothianidin reduces the efficacy of SumiShield® 50WG, a neonicotinoid formulation for indoor residual spraying, against Anopheles gambiae
Authors: Fouet Caroline , Ashu Fred , Ambadiang Marilène , Tchapga Williams , Wondji Charles , Kamdem Colince 
Published: April 2023

Abstract
Chronic exposure of mosquito larvae to pesticide residues in agricultural areas is often associated with evolution of resistance to insecticides used for vector control. This presents a concern for the efficacy of clothianidin, an agricultural neonicotinoid qualified for Indoor Residual Spraying (IRS). Using standard bioassays, we tested if reduced susceptibility to clothianidin affects the efficacy of SumiShield® 50WG, one of the two newly approved formulations, which contains 50% clothianidin. We simultaneously monitored susceptibility to clothianidin and to SumiShield® 50WG, testing adults of Anopheles gambiae, An. coluzzii and Culex sp collected from urban, suburban and agricultural areas of Yaoundé. We found that the level of susceptibility to the active ingredient predicted the efficacy of SumiShield® 50WG. This formulation was very potent against populations that achieved 100% mortality within 72 h of exposure to a discriminating dose of clothianidin. By contrast, mortality leveled off at 75.4 ± 3.5% within 7 days of exposure to SumiShield® 50WG in An. gambiae adults collected from a farm where spraying of acetamiprid and imidacloprid is driving cross-resistance to clothianidin. These findings indicate that more potent formulations of clothianidin or different insecticides should be prioritized in areas where resistance is emerging.
Association and Mechanism of KCNJ11 Gene Polymorphism with the Effects of High-Intensity Interval Training on Cardiopulmonary Function
Authors: Sun Ping , Chen Nan , Zheng Huifang , Wang Fangyuan , Zhang Sheping , Xia Xiaohui 
Published: April 2023

Abstract
Background The effects of exercise training exhibit individual variability and are significantly influenced by genetic factors. This study aims to investigate the association between KCNJ11 gene polymorphisms and changes in cardiopulmonary function after high-intensity interval training (HIIT) in healthy college students, to identify gene markers that are sensitive to HIIT intervention and to explore the biological mechanisms underlying the impact of polymorphic loci on phenotype.Methods A total of 259 healthy college students underwent 12 weeks of HIIT training, and changes in maximal oxygen uptake, anaerobic threshold, and efficiency index were measured before and after the training. Genotyping of KCNJ11 gene polymorphic loci was performed using microarray, and the plink software was used to analyze the association between changes in indicators before and after training and genotype. The Gene Cards database was searched, and the 3D SNP software was used to analyze the biological function of polymorphic loci.Results 1) The rs5219 locus in the KCNJ11 gene region was significantly associated with changes in maximal oxygen uptake before and after training (P = 0.015 < 0.05), and the T allele was the dominant genotype; rs5218 locus was significantly associated with changes in quantitative load heart rate before and after training (P = 0.04 < 0.05), and the G allele was the dominant genotype. 2) Expression quantitative trait locus (eQTL) analysis showed that in skeletal and cardiac muscle, the regulatory genes of the rs5219 locus were ABCC8, KCNC1, and the expression of T allele was lower than that of C allele; the regulatory genes of the rs5218 locus were ABCC8, KCNJ11, and the expression of G allele was lower than that of A allele. 3) The 3DSNP functional prediction showed that rs5219 was located in the same linkage disequilibrium block as a polymorphic locus with strong regulatory function.Conclusions The KCNJ11 gene SNPs rs5219-T and rs5218-G genotypes have a positive effect on the improvement of cardiopulmonary function after 12 weeks of HIIT training and can serve as molecular markers for predicting the effectiveness of HIIT training. Rs5219 and rs5218 are eQTL loci, and their biological mechanisms may be achieved through tissue-specific expression of polymorphic loci, long-distance regulation of target genes, and linkage disequilibrium mechanisms.
The Effects of ICT on Performance and Well-Being According to Employee Position Level in the Gabonese Civil Service: A Longitudinal Study
Authors: Medzo-M’engone Joseph , Chaumon Marc-Éric Bobillier , Préau Marie 
Published: April 2023

Abstract

Background We examined the impact of ICT demands on the performance and psychological well-being at work (PWBW) of civil servants. The moderating role of employee position levels was also assessed. Therefore, we hypothesised that the impact of ICT would predict the performance (H1) and PWBW (H2) of civil servants and that employee position levels would play a moderating role in the relationship between the impact of ICT on civil servants' performance and PWBW (H3).Methods A total of 367 civil servants from different public institutions (35.01% female; Mage = 37.44 years, SDage = 7.35) responded to the questionnaire at all stages. The Work Performance scale, the PWBW scale and the ICT Demands scale were applied to assess the impact of ICT on participants’ work performance and PWBW respectively. Data were analysed using repeated measures analysis of variance, hierarchical linear regression and structural equation modeling (SEM).Results The results showed that the impact of ICT was generally more positive among supervisors than among the officers in T2 (1 year later) predicting a higher level of performance and PWBW of supervisors than officers in T3 (2 years later). The results also showed that employee position levels moderated the relationship between ICT demands, performance, and PWBW.Conclusions The results of this study provide important insights into the existing literature and help practitioners in the world of work, particularly in developing countries, to better understand how the introduction of a technological tool into employee practices can engender individual attitudes, emotions and behaviours that are appropriate or inappropriate for the goals of the organisation and how the employee's perception of a technological artifact changes according to job level.
Could Bisphenol-A have a role in the etiology of neural tube defects?
Authors: Yenigül Nefise , Bektaş Nisa Aytaç , pakay Kaan , Dinçgez Burcu , Bilgin Elif Yuce , Budak Yasemin , ustunyurt emin 
Published: April 2023

Abstract
Objective: In this study, we aimed to evaluate BPA levels in the maternal serum and amniotic fluid of patients diagnosed with NTD. In addition, we wanted to investigate the relationship between neurodevelopmental defects, such as neural tube defects (NTD), and BPA levels.
Study design: The study consisted of 92 patients between the ages of 18-45 who had an amniocentesis at 15-22 weeks of gestation. The patients were divided into two groups according to the indications of amniocentesis. Maternal serum samples taken during the amniocentesis procedure of all patients were delivered to the biochemistry laboratory. The BPA values between groups were compared.
Results: In cases with NTD, amniotic fluid BPA levels and maternal blood BPA levels were significantly higher than the control group.
Conclusion: Plastic, which is indispensable for modern life, may negatively affect fetal development in intrauterine life. This study says that high maternal blood BPA may be associated with NTD.
3D model of the bronchial epithelial barrier to study repeated exposure to xenobiotics: application to silver nanoparticles
Authors: Chivé Chloé , Cord Claire Mc , Sanchez-Guzman Daniel , Brookes Oliver , Joseph Prinitha , Kuen René Lai , Phan Guillaume , Baeza-Squiban Armelle , Devineau Stéphanie , Boland Sonja 
Published: April 2023

Abstract

The use of animals in chemical testing and hazard assessment can be reduced using New Approach Methods or NAMs. In vitro human models have been successfully developed to measure the acute toxicity of drugs and environmental pollutants. In this study, we introduce the use of 3D model of the human bronchial epithelium to assess the repeated exposure to xenobiotics. The human lung epithelial cell line Calu-3 was exposed to silver nanoparticles at the air-liquid interface every 2 to 3 days during 12 days to mimic repeated exposure to inhaled particles. The biological effects of a single versus repeated exposure to silver nanoparticles were compared to determine whether transient, decreasing or increasing cellular responses were observed when exposing non-proliferating cells multiple times to a xenobiotic. Our results showed a stronger induction of the cellular response to metal stress after repeated exposure, and a stable oxidative stress response after a single or repeated exposure. While the epithelial barrier integrity was not affected, sustained translocation of silver was observed in the basal medium after each exposure. On the apical side, the proteomic analysis of the mucus revealed changes in the secreted protein profiles associated with the epithelial immune response after multiple exposure to silver nanoparticles that were unseen after a single exposure. These results demonstrate that it is possible and necessary to take into account the adaptive response of human cells using advanced NAMs to assess the toxicity of chemicals or environmental pollutants when chronic exposure is likely to occur.
Empirical Frequency Bound Derivation Reveals Prominent Mid-Frontal Alpha Associated with Neurosensory Dysfunction in Fragile X Syndrome
Authors: Pedapati Ernest V , Sweeney John A. , Schmitt Lauren M. , Ethridge Lauren E. , Miyakoshi Makoto , Liu Rui , Smith Elizabeth , Shaffer Rebecca C. , Wu Steve W. , Gilbert Donald L. , Horn Paul S. , Erickson Craig 
Published: April 2023

Abstract

The FMR1 gene is inactive in Fragile X syndrome (FXS), resulting in low levels of FMRP and consequent neurochemical, synaptic, and local circuit neurophysiological alterations in the fmr1 KO mouse. In FXS patients, electrophysiological studies have demonstrated a marked reduction in global alpha activity and regional increases in gamma oscillations associated with intellectual disability and sensory hypersensitivity. Since alpha activity is associated with a thalamocortical function with widely distributed modulatory effects on neocortical excitability, insight into alpha physiology may provide insight into systems-level disease mechanisms. Herein, we took a data-driven approach to clarify the temporal and spatial properties of alpha and theta activity in participants with FXS. High-resolution resting-state EEG data were collected from participants affected by FXS (n = 65) and matched controls (n = 70). We used a multivariate technique to empirically classify neural oscillatory bands based on their coherent spatiotemporal patterns. Participants with FXS demonstrated: 1) redistribution of lower-frequency boundaries indicating a “slower” dominant alpha rhythm, 2) an anteriorization of alpha frequency activity, and 3) a correlation of increased individualized alpha power measurements with auditory neurosensory dysfunction. These findings suggest an important role for alterations in thalamocortical physiology for the well-established neocortical hyper-excitability in FXS and, thus, a role for neural systems level disruption to cortical hyperexcitability that has been studied primarily at the local circuit level in animal models.
Tripartite Evolutionary Game in the Process of Network Attack and Defense
Authors: Chen Zigang , Fan Chaochao , Leng Tao , Chen Long , Liu Yuhong , Li Xiaoyong , Yuan Dongfeng 
Published: April 2023

Abstract

At present, the confrontation in cyberspace is becoming more and more fierce, and network attacks and defenses have become the main form of confrontation between parties in cyberspace. The maximum benefit can be obtained by using scientific decision-making. Traditional network attack and defense research mainly focuses on the relationship between network attackers and network defenders, but enough to explain the benefits of in the evolution of network attacks and defenses. In view of this, this paper constructs a tripartite evolutionary game model among network attackers, network defenders and government regulators, analyzes the evolutionary stability of each participant's strategy choice, discusses the influence of each element on the tripartite strategic choices, and further analyzes the stability of the equilibrium point of the tripartite evolutionary system. The research results show that (1) the timely supervision of the network by the government supervision department is conducive to enhancing the construction of network security and has strategic significance for the security of cyberspace; (2) the network defense department can obtain more effective defense measures when being attacked by the network; (3) the network defense party can analyze the strategic defense requirements that are more in line with its own benefits. Finally, we use Matlab2021a to conduct simulation analysis, verify the effectiveness of the evolutionary game model under different initial conditions, and put forward reasonable suggestions for network defenders and government regulators according to the analysis conclusions.
Dynamics of N6-methyladenosine modification during Alzheimer's disease development
Authors: Wang Yuqing , Li Xiaoyu , Yang Baozhi , Yuan Ti-Fei , Peng Bo , Rao Yanxia 
Published: April 2023

Abstract

N6-methyladenosine (m6A) modification is a common RNA modification in the central nervous system and has been linked to various neurological disorders, including Alzheimer's disease (AD). However, little is known about the dynamic of mRNA m6A modification and m6A enzymes during the development of AD.Therefore, this study examined the expression profiles of m6A and its enzymes in the development of AD. The results showed that changes in the expression levels of m6A regulatory factors occurred in the early stages of AD, indicating the potential involvement of m6A modification in disease onset. Moreover, the analysis of mRNA m6A expression profiles using m6A-seq revealed significant differences in m6A modification between AD and control brains.The differentially methylated genes were enriched in GO and KEGG terms related to processes such as inflammation response, immune system processes. And the differently expressed genes (DEGs) are negative associated with microglia homeostasis genes and but positive for “disease-associated microglia” (DAM) associated genes, suggesting that dysregulation of mRNA m6A modification may contribute to the development of AD by affecting the function and gene expression of microglia.
Assessment of attitudes and practices influencing adherence to seasonal malaria chemoprevention (SMC) in children &gt;5 years of age: The case of Rural Embangweni Mzimba district, Malawi.
Authors: Salangwa Chisomo , Lusale Dunny 
Published: April 2023

Abstract
Background: In the Sub-Saharan region, where malaria transmission is mainly seasonal, seasonal malaria chemoprevention (SMC) is the intermittent administration of a 3-day course of anti-malarial drugs during the months of maximum malaria risk. SMC is a useful intervention for lowering the frequency of simple and severe malaria episodes in children. However, morbidity cannot be decreased without treatment adherence. This study examined SMC medication adherence to understand caregiver attitudes and behaviours throughout the 2021 SMC campaign in rural Embangweni.
Methods: This study was carried out based on information gathered through random cluster sampling during independent monitoring. Adherent levels and caregiver attitudes and behaviours were assessed using information from caregiver self-reports and taxonomically analysable data.
Results:90.7% of caregivers (N = 300) knew that the medications help prevent malaria, and at the start of the treatment period, 90.7% of children (N = 300) received their first medication delivery. However, only 67% of caregivers (N = 200) stated that HSA was present when the treatment was initiated. 90% of kids (N = 300) and 81% (N = 219) of kids at the start of the treatment phase took their medication on the second and third days, respectively. The three primary excuses caretakers cited for stopping the three-day course of medication were "forgetting," "not having time," and "the mother's absence."
Conclusion: This quick, low-cost study showed that SMC coverage and caregiver adherence to complete the entire 3-day drug course were both high. The poll also revealed that some caregivers' understanding, attitudes, and practices surrounding medication adherence during the SMC campaign might be improved. Even greater adherence might result from enhancing training for distributors, developing and supplying them with tools for interpersonal communication, and tightening oversight.
Representation of prime numbers on the complex plane
Authors: Csóka Levente 
Published: April 2023

Abstract

A novel representation of a quasi-periodic prime numbers on the complex plan has been investigated. We focus on some quantities characterizing the sum of centres of complex way represented prime numbers. The results indicate that prime numbers can be generated by rooting of real numbers and progression can be decomposed into periodic sequences. The main approach is to root-decompose it into prime numbers. Basically, it is applied to extract hidden periodicities in quasi periodic prime series. The periodicities were arranged in parabolic spirals to show their harmonic pattern. Numerical evidence was provided to confirm that primes can be generated by rooting.
Parasitological Quality Evaluation of Pipe Waters in Abidjan, the Capital City of Cote D’ivoire
Authors: Clément Koffi Attoungbré , Karim Tuo , Sery Gonédélé Bi , Fiacre-Tanguy N’docho Assi , Landry N'Guessan Tiacoh , Karidja Yakoura Ouattara , Nina Amon Lydie , Béranger Ako Ako Aristide , Abo Henriette Vanga Bosson , Julien Coulibaly Kalpy , Offianan Toure André 
Published: April 2023

Background:
Treatment and wastewater disposal lack in Abidjan leads to wastewater discharge in the natural environment without treatment. These waters were loaded with pathogenic microorganisms that are the causes of many diseases.

Objectives:
This study aims to assess the parasitological quality of water from pipes of Abididjan City, studying protozoa and helminths.

Methodology:
400 samples were collected in three municipalities and 19 sites. Parasites were identified using sedimentation.

Results:
Biological analyzes revealed 269 (67.2%) positive samples, with the presence of 19 taxa belonging to helminths (nematodes, trematodes) and protozoa group (amoeba, ciliates, flagellates). Protozoa are most common with a clear dominance of amoebas class followed by ciliates and flagellates. In 269 positives samples, Entamoeba coli (160 (59.48%)), Endolimax nana (112 (41.64%)), Giardia spp (71 (26.4%)), and Paramecium caudatum (66 (24.54%)) are the most common species. The spatial distribution showed that Yopougon commune was the most parasitized, followed by Abobo-Adjame.
The most parasitized sites were CHU, SEL, and AG. At the seasonal level, the dry season was where the parasite load was highest regardless of municipality and sites.

Conclusion:
The presence of these parasite groups and the diversity of parasitic taxonomic indicates faecal contamination of piped water in Abidjan city. These parasites present a potential danger for these piped waters' direct and indirect uses. Therefore, it is necessary to treat this water before any use and before it flows into surface water to avoid these parasites.