bluuebunny/crossref_metadata_2025_split huggingface dataset:
A latent profile analysis linking career learning experience profile to career decision‐making difficulties
Authors: Chang Biru , Zekai Li , Wang Longjie , Zhong Li , Shen Yamei 
Published: May 2023

AbstractAlthough both theoretical and empirical studies have revealed the impact of career learning experiences on career decision‐making, most studies have ignored the interwind relationships between different career learning experiences. Thus, the present study employed a person‐centered approach to explore the different latent profiles of the career learning experiences that arise among Chinese college students and to examine how these profiles are related to career decision‐making difficulties. A sample of 1737 college students (72.7% females) completed the Chinese version of the Career Exploration and Decision Learning Experiences (CEDLE‐C) and Career Decision‐making Difficulties Questionnaire (CDDQ). Four career learning experience profiles were identified: the multi‐source profile (5%, 79) characterized by high levels of personal mastery experience (PME), verbal persuasion (VP), vicarious learning (VL), and positive emotion (PE) and very low level of negative emotion (NE), the high‐mid‐source profile (42%, 723) characterized by these experiences slightly above mean levels, the mid‐source profile (45%, 789) characterized by mean levels of these experiences, and the unconfident profile (8%, 146) characterized by very low levels of PME, VP, VL, PE, and high level of NE. These four profiles differed in career decision‐making difficulties. Students in the unconfident profile experienced the most career decision‐making difficulties. The implications of these findings for further research and practices are discussed.
Synthesis of a Benzotrisazulene via Trioxobenzotrisazulene
Authors: Kirschbaum Tobias , Rominger Frank , Mastalerz Michael 
Published: May 2023

AbstractPolycyclic aromatic hydrocarbons (PAHs) containing odd‐membered rings (such as pentagons or heptagons) are fascinating synthetic targets. A special case is the introduction of five‐ and seven‐membered rings in form of an azulene unit. Azulene itself is an aromatic compounds known for its deep blue color, which is a result of its internal dipole moment. When azulene is embedded into PAHs it can change the optoelectronic properties of the PAH significantly. Herein, the synthesis and characterization of a PAH containing three azulene units is reported via reduction and elimination of its trioxo derivative.
Gray‐Box Modeling of the Molecular Weight Distribution in a Batch Polymerization Reactor
Authors: Bordas Balazs , Kurt Kutup , Bamberg Andreas , Engell Sebastian 
Published: May 2023

AbstractWe introduce a gray‐box approach for modeling the molecular weight distribution in step‐growth polymerization reactions using the aggregation population balance equation. The approach is based on extracting a data‐based kernel function from in‐process measurements of the molecular weight distribution. The method is applied to historical data from an industrial batch polymerization reactor. The resulting model is used for decision support in production by predicting the reaction endpoint corresponding to a target molecular weight. The accuracy of the predictions proved to be sufficient for the deployment of the method.
Accelerated Ring‐Opening Polymerization of α‐Amino Acid N‐Carboxyanhydridevia Inorganic Nano‐initiators†
Authors: Zhu Junliang , He Yunjie , Ge Chenglong , Si Wenting , Song Ziyuan , Yin Lichen 
Published: May 2023

Comprehensive SummaryWhile the accelerated polymerization of N‐carboxyanhydrides (NCAs) has been utilized to synthesize versatile polypeptide materials in an efficient manner with minimized side reactions, the preparation of polypeptide‐based inorganic/organic hybrid materials with the acceleration strategy remained largely unexplored. Herein, we report the accelerated ring‐opening polymerization (ROP) of NCAs mediated by amine‐modified inorganic nano‐initiators, such as mesoporous silica nanoparticles (MSN‐NH2), which is driven by the cooperative effect of the neighboring α‐helical polypeptide chains in a dichloromethane (DCM)/water biphasic system. Well‐defined nano‐hybrids were prepared within 15 min from non‐purified NCA monomers, through in situ purification and subsequent ultrafast polymerization process. NCAs can be rapidly initiated by amino groups of MSN uniformly dispersed at the interface of DCM and water, and subsequently formed the well‐defined polypeptides within 15 min. The prepared inorganic/organic nano‐hybrid with MSN as the core and polypeptide as the shell adopted spherical morphology and uniform size distribution due to the excellent controllability of ROP. Besides, this system is also suitable for a variety of NCAs and inorganic nano‐initiators. This research allows efficient and rapid preparation of inorganic/organic nano‐hybrids, and further promotes the extensive application of this material in the biomedical fields.
One‐ and Two‐Electron Reductions in MiniSOG and their Implication in Catalysis**
Authors: Azpitarte Oksana , Zudaire Ane , Uranga Jon , Lopez Xabier , Salassa Luca , Formoso Elena , Rezabal Elixabete 
Published: May 2023

AbstractThe unconventional bioorthogonal catalytic activation of anticancer metal complexes by flavin and flavoproteins photocatalysis has been reported recently. The reactivity is based on a two‐electron redox reaction of the photoactivated flavin. Furthermore, when it comes to flavoproteins, we recently reported that site mutagenesis can modulate and improve this catalytic activity in the mini Singlet Oxygen Generator protein (SOG). In this paper, we analyze the reductive half‐reaction in different miniSOG environments by means of density functional theory. We report that the redox properties of flavin and the resulting reactivity of miniSOG is modulated by specific mutations, which is in line with the experimental results in the literature. This modulation can be attributed to the fundamental physicochemical properties of the system, specifically (i) the competition of single and double reduction of the flavin and (ii) the probability of electron transfer from the protein to the flavin. These factors are ultimately linked to the stability of flavin‘s electron‐accepting orbitals in different coordination modes.
Enam expression is regulated by Msx2
Authors: Ruspita Intan , Das Pragnya , Miyoshi Keiko , Noma Takafumi , Snead Malcolm L. , Bei Marianna 
Published: May 2023

AbstractBackgroundThe precise formation of mineralized dental tissues such as enamel and/or dentin require tight transcriptional control of the secretion of matrix proteins. Here, we have investigated the transcriptional regulation of the second most prominent enamel matrix protein, enamelin, and its regulation through the major odontogenic transcription factor, MSX2.ResultsUsing in vitro and in vivo approaches, we identified that (a) Enam expression is reduced in the Msx2 mouse mutant pre‐secretory and secretory ameloblasts, (b) Enam is an early response gene whose expression is under the control of Msx2, (c) Msx2 binds to Enam promoter in vitro, suggesting that enam is a direct target for Msx2 and that (d) Msx2 alone represses Enam gene expression.ConclusionsCollectively, these results illustrate that Enam gene expression is controlled by Msx2 in a spatio‐temporal manner. They also suggest that Msx2 may interact with other transcription factors to control spatial and temporal expression of Enam and hence amelogenesis and enamel biomineralization.
Generation and applications of simulated datasets to integrate social network and demographic analyses
Authors: Silk Matthew J. , Gimenez Olivier 
Published: May 2023

AbstractSocial networks are tied to population dynamics; interactions are driven by population density and demographic structure, while social relationships can be key determinants of survival and reproductive success. However, difficulties integrating models used in demography and network analysis have limited research at this interface. We introduce the R package genNetDem for simulating integrated network–demographic datasets. It can be used to create longitudinal social network and/or capture–recapture datasets with known properties. It incorporates the ability to generate populations and their social networks, generate grouping events using these networks, simulate social network effects on individual survival, and flexibly sample these longitudinal datasets of social associations. By generating co‐capture data with known statistical relationships, it provides functionality for methodological research. We demonstrate its use with case studies testing how imputation and sampling design influence the success of adding network traits to conventional Cormack–Jolly–Seber (CJS) models. We show that incorporating social network effects into CJS models generates qualitatively accurate results, but with downward‐biased parameter estimates when network position influences survival. Biases are greater when fewer interactions are sampled or fewer individuals observed in each interaction. While our results indicate the potential of incorporating social effects within demographic models, they show that imputing missing network measures alone is insufficient to accurately estimate social effects on survival, pointing to the importance of incorporating network imputation approaches. genNetDem provides a flexible tool to aid these methodological advancements and help researchers testing other sampling considerations in social network studies.
Short‐term toll‐like receptor 9 inhibition leads to left ventricular wall thinning after myocardial infarction
Authors: Lenz Max , Kiss Attila , Haider Patrick , Salzmann Manuel , Brekalo Mira , Krychtiuk Konstantin A. , Hamza Ouafa , Huber Kurt , Hengstenberg Christian , Podesser Bruno K. , Wojta Johann , Hohensinner Philipp J. , Speidl Walter S. 
Published: May 2023

AbstractAimsIschaemia–reperfusion injury (IRI) following myocardial infarction remains a challenging topic in acute cardiac care and consecutively arising heart failure represents a severe long‐term consequence. The extent of neutrophil infiltration and neutrophil‐mediated cellular damage are thought to be aggravating factors enhancing primary tissue injury. Toll‐like receptor 9 was found to be involved in neutrophil activation as well as chemotaxis and may represent a target in modulating IRI, aspects we aimed to illuminate by pharmacological inhibition of the receptor.Methods and resultsForty‐nine male adult Sprague–Dawley rats were used. IRI was induced by occlusion of the left coronary artery and subsequent snare removal after 30 min. Oligonucleotide (ODN) 2088, a toll‐like receptor 9 (TLR9) antagonist, control‐ODN, or DNase, were administered at the time of reperfusion and over 24 h via a mini‐osmotic pump. The hearts were harvested 24 h or 4 weeks after left coronary artery occlusion and immunohistochemical staining was performed. Echocardiography was done after 1 and 4 weeks to determine ventricular function. Inhibition of TLR9 by ODN 2088 led to left ventricular wall thinning (P = 0.003) in association with drastically enhanced neutrophil infiltration (P = 0.005) and increased markers of tissue damage. Additionally, an up‐regulation of the chemotactic receptor CXCR2 (P = 0.046) was found after TLR9 inhibition. No such effects were observed in control‐ODN or DNase‐treated animals. We did not observe changes in monocyte content or subset distribution, hinting towards neutrophils as the primary mediators of the exerted tissue injury.ConclusionsOur data indicate a TLR9‐dependent, negative regulation of neutrophil infiltration. Blockage of TLR9 appears to prevent the down‐regulation of CXCR2, followed by an uncontrolled migration of neutrophils towards the area of infarction and the exertion of disproportional tissue injury resulting in potential aneurysm formation. In comparison with previous studies conducted in TLR−/− mice, we deliberately chose a transient pharmacological inhibition of TLR9 to highlight effects occurring in the first 24 h following IRI.
FBXW7 promotes autophagy and inhibits proliferation of oral squamous cell carcinoma
Authors: Qiu Bo , Sun Yang , Nie Wei , Yang Qi , Guo Xiangjun 
Published: May 2023

AbstractBackgroundF‐box and WD repeat domain containing 7 (FBXW7) is a critical tumor suppressor. The expression of FBXW7 is decreased in oral squamous cell carcinoma (OSCC) tissues and shows diagnosis value. We aimed to investigate the influence of FBXW7 overexpression on OSCC cell proliferation and autophagy.MethodsIn Balb/c nude mice, CAL27 xenograft tumor model was established. Western blot was employed to evaluate protein level. Messenger RNA level was analyzed by quantitative reverse transcription‐polymerase chain reaction. Colony formation assay and MTT assay were employed to evaluate cell proliferation.ResultsFBXW7 expression was decreased in OSCC cell lines. FBXW7 inhibited cell proliferation of SCC9 and CAL27. FBXW7 increased Autophagy related 7 (Atg7), Beclin1 (BECN1), B‐cell lymphoma 2 (BCL2) ‐associated X (BAX), BCL2 antagonist killer (BAK), and microtubule‐associated protein 1 light chain 3 (LC3) levels and decreased MCL1 and BCL2 levels in CAL27 cells. FBXW7 decreased tumor volume and weight in CAL27 xenograft tumor model. FBXW7 increased BECN1, Atg7, and LC3 levels in CAL27 xenograft tumor model.ConclusionIn conclusion, decreased expression of FBXW7 is confirmed in diverse OSCC cell lines. The enhanced FBXW7 expression inhibits cancer cell proliferation and promotes autophagy in both OSCC cells and xenograft tumor model.
Relative Effects of Radiation‐Induced Changes in Bone Mass, Structure, and Tissue Material on Vertebral Strength in a Rat Model
Authors: Emerzian Shannon R , Wu Tongge , Vaidya Rachana , Tang Simon Y , Abergel Rebecca J , Keaveny Tony M 
Published: May 2023

ABSTRACTThe observed increased risk of fracture after cancer radiation therapy is presumably due to a radiation‐induced reduction in whole‐bone strength. However, the mechanisms for impaired strength remain unclear, as the increased fracture risk is not fully explained by changes in bone mass. To provide insight, a small animal model was used to determine how much of this whole‐bone weakening effect for the spine is attributable to changes in bone mass, structure, and material properties of the bone tissue and their relative effects. Further, because women have a greater risk of fracture after radiation therapy than men, we investigated if sex had a significant influence on bone's response to irradiation. Fractionated in vivo irradiation (10 × 3 Gy) or sham irradiation (0 Gy) was administered daily to the lumbar spine in twenty‐seven 17‐week‐old Sprague–Dawley rats (n = 6–7/sex/group). Twelve weeks after final treatment, animals were euthanized, and lumbar vertebrae (L4and L5) were isolated. Using a combination of biomechanical testing, micro‐CT‐based finite element analysis, and statistical regression analysis, we separated out the effect of mass, structural, and tissue material changes on vertebral strength. Compared with the sham group (mean ± SD strength = 420 ± 88 N), the mean strength of the irradiated group was lower by 28% (117 N/420 N,p < 0.0001). Overall, the response of treatment did not differ with sex. By combining results from both general linear regression and finite element analyses, we calculated that mean changes in bone mass, structure, and material properties of the bone tissue accounted for 56% (66 N/117 N), 20% (23 N/117 N), and 24% (28 N/117 N), respectively, of the overall change in strength. As such, these results provide insight into why an elevated clinical fracture risk for patients undergoing radiation therapy is not well explained by changes in bone mass alone. © 2023 The Authors.Journal of Bone and Mineral Researchpublished by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Oncogenic role of an uncharacterized cold‐induced zinc finger protein 726 in breast cancer
Authors: Bandyopadhayaya Shreetama , Yadav Pooja , Sharma Ankit , Dey Sanjay Kumar , Nag Alo , Maheshwari Rekha , Ford Bridget M. , Mandal Chandi C. 
Published: May 2023

AbstractThe unobtrusive cold environmental temperature can be linked to the development of cancer. This study, for the first time, envisaged cold stress‐mediated induction of a zinc finger protein 726 (ZNF726) in breast cancer. However, the role of ZNF726 in tumorigenesis has not been defined. This study investigated the putative role of ZNF726 in breast cancer tumorigenic potency. Gene expression analysis using multifactorial cancer databases predicted overexpression of ZNF726 in various cancers, including breast cancer. Experimental observations found that malignant breast tissues and highly aggressive MDA‐MB‐231 cells showed an elevated ZNF726 expression as compared to benign and luminal A type (MCF‐7), respectively. Furthermore, ZNF726 silencing decreased breast cancer cell proliferation, epithelial–mesenchymal transition, and invasion accompanied by the inhibition of colony‐forming ability. Concordantly, ZNF726 overexpression significantly demonstrated opposite outcomes than ZNF726 knockdown. Taken together, our findings propose cold‐inducible ZNF726 as a functional oncogene demonstrating its prominent role in facilitating breast tumorigenesis. An inverse correlation between environmental temperature and total serum cholesterol was observed in the previous study. Furthermore, experimental outcomes illustrate that cold stress elevated cholesterol content hinting at the involvement of the cholesterol regulatory pathway in cold‐induced ZNF726 gene regulation. This observation was bolstered by a positive correlation between the expression of cholesterol‐regulatory genes and ZNF726. Exogenous cholesterol treatment elevated ZNF726 transcript levels while knockdown of ZNF726 decreased the cholesterol content via downregulating various cholesterol regulatory gene expressions (e.g., SREBF1/2, HMGCoR, LDLR). Moreover, an underlying mechanism supporting cold‐driven tumorigenesis is proposed through interdependent regulation of cholesterol regulatory pathway and cold‐inducible ZNF726 expression.
An eco‐friendly synthesis of 5‐aminotetrazoles using trichloroisocyanuric acid as desulfurization agent of thioureas
Authors: Moraes Adriana Marques , da Silva Tiago Lima , de Mattos Marcio C. S. 
Published: May 2023

AbstractA novel procedure for the preparation of various 5‐aminotetrazoles derivatives was achieved using trichloroisocyanuric acid as a desulfurizing agent of thioureas and trimethylsilyl azide in ethyl acetate at room temperature. This methodology provided substituted 5‐aminotetrazoles through a more sustainable reaction using an affordable, safe, stable, and easily handled N‐halo reagent, a safer azide source, and an environmentally friendly solvent.
Biogenic synthesized copper oxide nanoparticles by Bacillus subtilis: Investigating antibacterial activity on the mexAB‐oprM efflux pump genes and cytotoxic effect on MCF‐7 cells
Authors: Azizi Hossein , Akbari Neda , Kheirandish Farnaz , Sepahvand Asghar 
Published: May 2023

AbstractOne of the main characteristics of Pseudomonas aeruginosa is remarkable intrinsic antibiotic resistance which is associated with production of β‐lactamases and the expression of inducible efflux pumps. Nanoparticles (NPs) are a novel option for coping with this resistant bacteria. Hence, the aim of present study was production of CuO NPs via Bacillus subtilis and applied them to deal with resistant bacteria. For this purpose, first NPs were synthesized and were analyzed with different standard techniques containing scanning electron microscope, Fourier‐transform infrared spectroscopy, and X‐ray powder diffraction. Microdilution Broth Method and real‐time polymerase chain reaction were used to antibacterial properties of the CuO NPs and expression of mexAB‐oprM in clinical samples of P. aeruginosa, respectively. The cytotoxic effect of CuO NPs was also evaluated on MCF7 as a breast cancer cell line. Finally, the data were analyzed by one‐way analysis of variance and Tukey's tests. The size of CuO NPs was in the range of 17–26 nm and showed antibacterial effect at <1000 μg/mL concentrations. Our evidence noted that the antibacterial effects of the CuO NPs occurred through the downregulation of mexAB‐oprM and upregulation of mexR. The interesting point was that CuO NPs had an inhibitory effect on MCF7 cell lines with the optimal inhibition concentration at IC50 = 25.73 µg/mL. Therefore, CuO NPs can be considered as a promising medical candidate in the pharmaceutical industry.
Corporate social responsibility: Insights from COVID‐19 and stakeholder theory
Authors: Bansal Sanchita , Garg Isha , Singh Shifali 
Published: May 2023

AbstractThe transition from primary sustainable goals to crisis management exemplifies a new era of corporate social responsibility, sustainable business models, corporate sustainability, and stakeholder theory. This study examines the varied dynamics of corporate social responsibilities (CSR) during COVID‐19, as well as its potential and limitations, in order to gain a better understanding of CSR. The results expand upon the instrumental version of CSR and the application of stakeholder theory during COVID‐19. It reflects on the necessity for a wider integration of societal issues in CSR's driving philosophy as well as the underlying need to study diverse sectors of governance across the globe due to the increased potential for exploitation of the weak, particularly during times of crisis. This study examines the theoretical foundations of the themes and the lines of divergence between CSR's past and present by reviewing the social, intellectual, and conceptual structure of the literature. It emphasizes the importance of post‐COVID‐19 policies that prioritize job creation by implementing stronger labor market standards. Governments should eliminate barriers and implement pro‐SME laws and programs. A sustainable fiscal policy takes future generations into account. Sustainable corporate finance incorporates long‐term financial goals and social values into stakeholder theory.
The same parastomal hernia repairs rate in the different approaches to colostomy
Authors: Xiao Jianlin , Shen Yu , Yang Xuyang , Zeng Hanjiang , Wei Mingtian , Meng Wenjian , Wang Ziqiang 
Published: May 2023

AbstractPurposeThis study aimed to compare the parastomal hernia repairs rate in the different approaches to colostomy and investigate the risk factors for parastomal hernia formation in patients with permanent colostomies.MethodsConsecutive rectal cancer patients who underwent abdominoperineal resection from June 2014 to July 2020 in West China Hospital were divided into two groups according to their surgical approach for permanent colostomies. The impact of different approaches to colostomy on parastomal hernia repairs was determined by comparing a group of patients receiving an extraperitoneal route to colostomy with a group receiving transperitoneal. Potential variables were evaluated first with univariate and then multivariate analyses to identify the risk factors for the formation of parastomal hernia.ResultsTwo hundred two subjects in the transperitoneal group and 103 in the extraperitoneal group attended the follow‐up visit with a median follow‐up period of 33 (25th–75th percentiles, 17–46) months. Clinically and radiologically detectable parastomal hernias were present in 76 of 202 (37.6%) and 14 of 103 (13.6%) subjects in the transperitoneal and extraperitoneal groups during the follow‐up period (p＜0.01). Besides, 10 of 76 (13.1%) subjects in the transperitoneal group and 2 of 14 (14.3%) subjects in the extraperitoneal group underwent a parastomal hernia operation during the follow‐up (p = 0.82). In addition, the transperitoneal approach of colostomy (p = 0.002), older age (p＜0.001), and higher body mass index (p = 0.013) were identified as independent risk factors for the occurrence of parastomal hernia.ConclusionsExtraperitoneal colostomy decreased the detectable parastomal hernias but did not reduce the surgical repair rate of parastomal hernias.