bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beeswax as a natural alternative to synthetic waxes for fabrication of PLA/diatomaceous earth composites
Authors: Dobrosielska Marta , Dobrucka Renata , Kozera Paulina , Brząkalski Dariusz , Gabriel Ewa , Głowacka Julia , Jałbrzykowski Marek , Kurzydłowski Krzysztof J. , Przekop Robert E. 
Published: January 2023

AbstractIn this study, injection moulding was applied to produce biocomposites consisting of polylactide (PLA) and amorphous diatomaceous earth used as a filler at different concentrations. Natural wax and synthetic wax were added to improve processing properties, comparing the resulting biocomposites. The use of natural beeswax makes the composite environmentally friendly. The prepared composites contained 2.5, 5, 10 and 15% w/w filler. The test samples have been injection moulded. Rheological, mechanical, surface and other properties were assessed for the fabricated composites. The testing has shown that the use of wax additives has a significant influence on the mechanical properties (tensile strength, flexural strength, impact strength) and the hydrophilicity/hydrophobicity of composite surfaces. The addition of natural wax, especially at lower concentration, has a positive effect on the rheological properties of composites (melt flow rate, MFR), flexural modulus and impact strength. Different composite parameters are modified by different wax types so both natural and synthetic waxes, can be used interchangeably, depending on the required final material characteristics.
Cellular senescence affects energy metabolism, immune infiltration and immunotherapeutic response in hepatocellular carcinoma
Authors: Gao Biao , Wang Yafei , Lu Shichun 
Published: January 2023

AbstractAging is an inevitable consequence of life, characterized by a progressive decline in tissue and organ function and an increased risk of death. There is growing evidence that aging is closely related to tumor development and immune regulation. However, in hepatocellular carcinoma, the relationship between cellular senescence and immune infiltration, energy metabolism, chemokines, and immunotherapeutic response is unclear and needs further study. We first analyzed 274 cellular senescence-associated genes by the NMF algorithm and identified two cellular senescence-associated clusters. Subsequently, we compared the differences between the two clusters, in terms of immune infiltration, energy metabolism, chemokines, and immunotherapeutic response to treatment. We further constructed risk models using cellular senescence-associated signature genes that could effectively identify the two subpopulations. Finally, we validated the validity and robustness of the risk model using an external dataset. We found significant differences in survival prognosis between two cellular senescence-associated clusters. In addition, we found significant differences in immune cell infiltration, expression of energy metabolism-related genes, expression of chemokine-related genes, expression of immune checkpoint-related genes, Tumor Immune Dysfunction and Exclusion between the two clusters. Also, a scoring system associated with cellular senescence was developed and validated as an independent prognostic indicator. It was validated as an independent prognostic factor and immunotherapeutic predictor for HCC. It was validated as an independent prognostic factor and immunotherapeutic predictor for HCC. The cellular senescence-related scoring system was validated as an independent prognostic factor and immunotherapy predictor for HCC, and patients with low CSS were characterized by prolonged survival time. Our study confirmed the relationship between cellular senescence and immune cell infiltration, energy metabolism, chemokines, expression of immune checkpoint-related genes, and response to immunotherapy. This enhances our understanding of cellular senescence and tumor immune microenvironment, energy metabolism, chemokines, and provides new insights to improve immunotherapy outcomes in HCC patients. It provides new insights to improve the outcome of immunotherapy in HCC patients.
Serum cortisol and insulin-like growth factor 1 levels in major depressive disorder and schizophrenia
Authors: Arinami Hiroshi , Watanabe Yuichiro , Suzuki Yutaro , Tajiri Misuzu , Tsuneyama Nobuto , Someya Toshiyuki 
Published: January 2023

AbstractThe pathophysiology underlying major depressive disorder (MDD) and schizophrenia is related to endocrine system functions and includes changes in the blood levels of cortisol and insulin-like growth factor 1 (IGF-1). However, these hormones have not been investigated simultaneously in patients with MDD and schizophrenia. We investigated the differences in serum cortisol and IGF-1 levels among patients with MDD and schizophrenia and controls. We included 129 patients with MDD, 71 patients with schizophrenia, and 71 healthy volunteers. Blood tests were performed between 6:00 am and 11:00 am after fasting. Serum cortisol levels were significantly higher in patients with schizophrenia than in patients with MDD and controls. Serum cortisol levels were significantly higher in patients with MDD than in controls. Serum IGF-1 levels were higher in both patient groups than in controls, whereas there was no significant difference between patients with MDD and schizophrenia. Both cortisol and IGF-1 levels were positively correlated with the Hamilton Rating Scale for Depression score in patients with MDD, whereas cortisol level was positively correlated and IGF-1 level was negatively correlated with the Brief Psychiatric Rating Scale score in patients with schizophrenia. The differences in the level of these hormones suggest pathophysiological differences between these disorders.
Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling
Authors: Schoger Eric , Bleckwedel Federico , Germena Giulia , Rocha Cheila , Tucholla Petra , Sobitov Izzatullo , Möbius Wiebke , Sitte Maren , Lenz Christof , Samak Mostafa , Hinkel Rabea , Varga Zoltán V. , Giricz Zoltán , Salinas Gabriela , Gross Julia C. , Zelarayán Laura C. 
Published: January 2023

AbstractAberrant Wnt activation has been reported in failing cardiomyocytes. Here we present single cell transcriptome profiling of hearts with inducible cardiomyocyte-specific Wnt activation (β-catΔex3) as well as with compensatory and failing hypertrophic remodeling. We show that functional enrichment analysis points to an involvement of extracellular vesicles (EVs) related processes in hearts of β-catΔex3 mice. A proteomic analysis of in vivo cardiac derived EVs from β-catΔex3 hearts has identified differentially enriched proteins involving 20 S proteasome constitutes, protein quality control (PQC), chaperones and associated cardiac proteins including α-Crystallin B (CRYAB) and sarcomeric components. The hypertrophic model confirms that cardiomyocytes reacted with an acute early transcriptional upregulation of exosome biogenesis processes and chaperones transcripts including CRYAB, which is ameliorated in advanced remodeling. Finally, human induced pluripotent stem cells (iPSC)-derived cardiomyocytes subjected to pharmacological Wnt activation recapitulated the increased expression of exosomal markers, CRYAB accumulation and increased PQC signaling. These findings reveal that secretion of EVs with a proteostasis signature contributes to early patho-physiological adaptation of cardiomyocytes, which may serve as a read-out of disease progression and can be used for monitoring cellular remodeling in vivo with a possible diagnostic and prognostic role in the future.
Alarm communication predates eusociality in termites
Authors: Sillam-Dussès David , Jandák Vojtěch , Stiblik Petr , Delattre Olivier , Chouvenc Thomas , Balvín Ondřej , Cvačka Josef , Soulet Delphine , Synek Jiří , Brothánek Marek , Jiříček Ondřej , Engel Michael S. , Bourguignon Thomas , Šobotník Jan 
Published: January 2023

AbstractTermites (Blattodea: Isoptera) have evolved specialized defensive strategies for colony protection. Alarm communication enables workers to escape threats while soldiers are recruited to the source of disturbance. Here, we study the vibroacoustic and chemical alarm communication in the wood roach Cryptocercus and in 20 termite species including seven of the nine termite families, all life-types, and all feeding and nesting habits. Our multidisciplinary approach shows that vibratory alarm signals represent an ethological synapomorphy of termites and Cryptocercus. In contrast, chemical alarms have evolved independently in several cockroach groups and at least twice in termites. Vibroacoustic alarm signaling patterns are the most complex in Neoisoptera, in which they are often combined with chemical signals. The alarm characters correlate to phylogenetic position, food type and hardness, foraging area size, and nesting habits. Overall, species of Neoisoptera have developed the most sophisticated communication system amongst termites, potentially contributing to their ecological success.
Single-cell RNA binding protein regulatory network analyses reveal oncogenic HNRNPK-MYC signalling pathway in cancer
Authors: Zhou Weiwei , Jie Qiuling , Pan Tao , Shi Jingyi , Jiang Tiantongfei , Zhang Ya , Ding Na , Xu Juan , Ma Yanlin , Li Yongsheng 
Published: January 2023

AbstractRNA-binding proteins (RBPs) are key players of gene expression and perturbations of RBP-RNA regulatory network have been observed in various cancer types. Here, we propose a computational method, RBPreg, to identify the RBP regulators by integration of single cell RNA-Seq (N = 233,591) and RBP binding data. Pan-cancer analyses suggest that RBP regulators exhibit cancer and cell specificity and perturbations of RBP regulatory network are involved in cancer hallmark-related functions. We prioritize an oncogenic RBP-HNRNPK, which is highly expressed in tumors and associated with poor prognosis of patients. Functional assays performed in cancer cells reveal that HNRNPK promotes cancer cell proliferation, migration, and invasion in vitro and in vivo. Mechanistic investigations further demonstrate that HNRNPK promotes tumorigenesis and progression by directly binding to MYC and perturbed the MYC targets pathway in lung cancer. Our results provide a valuable resource for characterizing RBP regulatory networks in cancer, yielding potential biomarkers for precision medicine.
Critical impacts of interfacial water on C–H activation in photocatalytic methane conversion
Authors: Sato Hiromasa , Ishikawa Atsushi , Saito Hikaru , Higashi Taisuke , Takeyasu Kotaro , Sugimoto Toshiki 
Published: January 2023

AbstractOn-site and on-demand photocatalytic methane conversion under ambient conditions is one of the urgent global challenges for the sustainable use of ubiquitous methane resources. However, the lack of microscopic knowledge on its reaction mechanism prevents the development of engineering strategies for methane photocatalysis. Combining real-time mass spectrometry and operando infrared absorption spectroscopy with ab initio molecular dynamics simulations, here we report key molecular-level insights into photocatalytic green utilization of methane. Activation of the robust C–H bond of methane is hardly induced by the direct interaction with photogenerated holes trapped at the surface of photocatalyst; instead, the C–H activation is significantly promoted by the photoactivated interfacial water species. The interfacial water hydrates and properly stabilizes hydrocarbon radical intermediates, thereby suppressing their overstabilization. Owing to these water-assisted effects, the photocatalytic conversion rates of methane under wet conditions are dramatically improved by typically more than 30 times at ambient temperatures (~300 K) and pressures (~1 atm) in comparison to those under dry conditions. This study sheds new light on the role of interfacial water and provides a firm basis for design strategies for non-thermal heterogeneous catalysis of methane under ambient conditions.
Activity and composition of the hyperactive comet 46P/Wirtanen during its close approach in 2018
Authors: Moulane Y. , Jehin E. , Manfroid J. , Hutsemékers D. , Opitom C. , Shinnaka Y. , Bodewits D. , Benkhaldoun Z. , Jabiri A. , Hmiddouch S. , Vander Donckt M. , Pozuelos F. J. , Yang B. 
Published: January 2023

Context. Hyperactive comets are a small group of comets whose activity is higher than expected. They seem to emit more water than would normally be expected given the size of their nucleus. Comet 46P/Wirtanen (hereafter, 46P) is among these objects of interest. Investigating its activity and composition evolution could provide clues about its origins and its formation region in the Solar nebulae.
Aims. Given the exceptional close approach of comet 46P to the Earth in 2018, we aim to study the evolution of its activity and composition as a function of heliocentric distances before and after perihelion.
Methods. We used both TRAPPIST telescopes to monitor the comet for almost a year with broad and narrow-band filters. We derived the production rates of five gaseous species (OH, NH, CN, C3, and C2) using a Haser model as well as the A(θ)fρ dust proxy parameter. The comet was also observed with the two optical high-resolution spectrographs UVES and ESPRESSO, mounted on the 8-m ESO VLT, to measure the isotopic ratios of C and N, along with the oxygen forbidden-line ratios and the NH2 ortho-to-para ratios.
Results. Over nearly a year, we followed the rise and decline of the production rates of different species as well as the dust activity of 46P on both pre- and post-perihelion. Relative abundances with respect to CN and OH along the orbit of the comet show constant and symmetric abundance ratios and a typical coma composition. We determined the rotation period of the nucleus using high-cadence observations and long series of CN images on several nights. We obtained a value of (9.18±0.05) hours at perihelion. Using the high-resolution spectra of 46P coma, we derived C and N isotopic ratios of 100±20 and 150±30 as well as a green-to-red forbidden oxygen [OI] line ratio of 0.23±0.02. We measured a NH2 ortho-to-para ratio of 3.31 ±0.03 and derived an ammonia ratio of 1.19±0.03, corresponding to a spin temperature of 27±1 K.
Conclusions. Narrow-band observations show that comet 46P is a hyperactive comet for which 40% of its nucleus surface is active. It has a typical composition, similar to other normal comets; however, an asymmetric behavior with respect to perihelion has been seen in its activity, which is typical of seasonal effects. Photometric measurements show no evidence for a change in the rotation period of the nucleus during this apparition. High-resolution spectra show that 46P has typical NH2 ortho-to-para, [OI] lines ratios, and C and N isotopic ratios.
Millimetre and sub-millimetre spectroscopy of doubly deuterated acetaldehyde (CHD2CHO) and first detection towards IRAS 16293-2422
Authors: Ferrer Asensio J. , Spezzano S. , Coudert L. H. , Lattanzi V. , Endres C. P. , Jørgensen J. K. , Caselli P. 
Published: January 2023

Context. The abundances of deuterated molecules with respect to their main isotopologue counterparts have been determined to be orders of magnitude higher than expected from the cosmic abundance of deuterium relative to hydrogen. The increasing number of singly and multi-deuterated species detections helps us to constrain the interplay between gas-phase and solid-state chemistry and to understand better deuterium fractionation in the early stages of star formation. Acetaldehyde is one of the most abundant complex organic molecules (COMs) in star-forming regions and its singly deuterated isotopologues have already been observed towards protostars.
Aims. A spectroscopic catalogue for astrophysical purposes is built for doubly deuterated acetaldehyde (CHD2CHO) from measurements in the laboratory. With this accurate catalogue, we aim to search for and detect this species in the interstellar medium and retrieve its column density and abundance.
Methods. Sub-millimetre wave transitions were measured for the non-rigid doubly deuterated acetaldehyde CHD2CHO displaying hindered internal rotation of its asymmetrical CHD2 methyl group. An analysis of a dataset consisting of previously measured microwave transitions and of the newly measured ones was carried out with an effective Hamiltonian which accounts for the tunnelling of the asymmetrical methyl group.
Results. A line position analysis was carried out, allowing us to reproduce 853 transition frequencies with a weighted root mean square standard deviation of 1.7, varying 40 spectroscopic constants. A spectroscopic catalogue for astrophysical purposes was built from the analysis results. Using this catalogue, we were able to detect, for the first time, CHD2CHO towards the low-mass proto-stellar system IRAS 16293-2422 utilising data from the ALMA Proto-stellar Interferometric Line Survey.
Conclusions. The first detection of the CHD2CHO species allowed for the derivation of its column density with a value of 1.3×1015 cm−2 and an uncertainty of 10–20%. The resulting D2/D ratio of ~20% is found to be coincident with D2/D ratios derived for other COMs towards IRAS 16293-2422, pointing to a common formation environment with enhanced deuterium fractionation.
Effect of protonation on the UV/VUV photostability of cyano-substituted anthracene and phenanthrene
Authors: Jacovella Ugo , Giuliani Alexandre , Hansen Christopher S. , Trevitt Adam J. , Nahon Laurent , Malloci Giuliano , Mulas Giacomo 
Published: January 2023

Context. The vacuum ultraviolet (VUV) photoprocessing of polycyclic aromatic hydrocarbons (PAHs) has been established as a key piece of the puzzle to understand the life cycle of carbon-based molecules in space. The recent detection of cyano (CN) aromatic species, with unexpectedly high abundance, motivated the current study of investigating their interaction with UV/VUV radiation.
Aims. The aims were to investigate the fate, after VUV photoexcitatation, of medium-size (three rings) CN-PAH radical cations and of their protonated analogs, and thus to assess the effect of protonation on the photostability of the CN-PAHs. Photoproducts (ionic fragments and dications) were mass-analyzed and measured as a function of photon energy. The results were also compared with those for the bare anthracene radical cation to assess the influence of the added CN group.
Methods. The positively charged CN-PAHs were stored in a quadrupole ion trap prior to interrogation by UV/VUV radiation, with photon energies between 4.5 and 13.6 eV, delivered by the DESIRS beamline from the synchrotron SOLEIL.
Results. The HCN/HNC loss channel is present for both radical cations and protonated species, but H2 loss is only apparent for the radical cations. Based on comparison with quantum chemical calculations, radiative and/or collisional processes should be relevant at energies lower than 8 eV, with a stronger propensity for radical cation than protonated CN-PAHs. The cata-condensed 9-CN-anthracene has a nearly two-fold larger photoionization yield at 13.6 eV than peri-condensed 9-CN-phenanthrene.
Conclusions. The photoionization yield of singly and doubly ionized CN-PAHs is greater for radical cations than for protonated analogs. The photoionization yields of CN-PAHs is diminished by protonation and, in the future, similar investigations should target larger protonated CN-PAHs to support a general model for the photo-processing of these relevant molecular systems. Similar processes to those for the bare PAH radical cations may involve the radical cations of CN-PAHs, making their addition important in models that describe the photoelectric heating of interstellar gas.
Strength and Wear Behavior of Three-Dimensional Printed and Prefabricated Denture Teeth: An In Vitro Comparative Analysis
Authors: Gad Mohammed M. , Alalawi Haidar , Akhtar Sultan , Al-Ghamdi Raghad , Alghamdi Rahaf , Al-Jefri Alaa , Al-Qarni Faisal D. 
Published: January 2023

Abstract
Objectives With advanced technology for complete denture fabrication, there is a lack of knowledge on the mechanical behavior of three-dimensional (3D) printed teeth despite the development of complete denture fabrication technologies. This study aimed to compare different types of 3D-printed teeth in terms of wear and fracture resistance in comparison to control prefabricated denture teeth.
Materials and Methods One prefabricated tooth was selected and fixed in a resin holder and half of the tooth remained in anatomic form, while the other half was flattened for the wear test. One from each type was scanned and then printed with different resins; Asiga (DentaTOOTH, Asiga, Alexandria 2015,NSW, Australia), FormLabs (Denture Base LP, FormLabs, Berlin, Germany), and NextDent (NextDent C&B MFH, NextDent B.V., Soesterberg, the Netherlands) according to manufacturer recommendations. A total of 60 specimens (20/resin, n = 10) were thermo cycled (5,000 cycles) and wear test samples were further subjected to cyclic loading (1,70,000 cycles) in a chewing simulator machine CS-4.2 (SD Mechatronik GmbH, Germany). The fracture strength of anatomic teeth was measured using a universal testing machine (Instron model 5965, Massachusetts, United States), while Geomagic Control X software was used to assess the amount of wear of flattened teeth. Statistical analyses were performed with one-way analysis of variance with Tukey's post hoc test at significance level of α = 0.05.
Results NextDent specimens showed the greatest volume loss, whereas FormLabs specimens showed the least volume loss. Comparing NextDent specimens to FromLabs specimens, FromLabs showed statistically significantly less volume loss (p < 0.001). No other group pairs differed significantly from one another in terms of volume loss (p > 0.06).
Conclusion 3D-printed denture teeth showed comparable strength and wear resistance with the prefabricated denture teeth and were suitable for long-term clinical usage except for NextDent that significantly showed the lowest fracture resistance.