bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Corn oligopeptide on Dexamethasone-induced muscle atrophy in Sprague-Dawley rats
Authors: Wang Yunfeng , Yan Jiuming , Shi Lei , Zhou Zhengming , Guo Jiankui , Wei Yaoyao , Hu Wen 
Published: December 2022

Abstract
Corn oligopeptide (COP) derived from corn gluten meal (CGM) was proved to possess plenty of biological properties. However, there is no scientific evidence to support that COP can ameliorate muscle atrophy. To find a new therapeutic nutritional substrate of muscle atrophy, we investigated the anti-myopathy effects of COP in DEX-inducted rat muscle atrophy model and tried to explore the underlying mechanisms. Co-administration of COP (2.0g/kg.bw) with DEX for 5 weeks reversed DEX-induced reduction in gastrocnemius (Gas) and plantaris (Pla) muscles weight, grip strength, exercise tolerance and myofiber cross sectional area (CSA) of soleus (Sol), up-regulated the protein levels of Phospho-Akt and mTOR that are closely relate to protein synthesis, and down-regulated the expression of FoxO3a, NF-κBp65, Atrogin-1, MuRF-1, LC3 and ULK1 that are the key components of Ubiquitin–proteasome system and Autophagy that activate proteolysis. In addition, according to muscles weight, grip strength, exercise tolerance changing over experiment time, we found the three indexes were all improved at 3rd week. Therefore, we conclude that the optimal duration for COP to improve DEX-induced muscle atrophy might be 3 weeks, and mechanistically, COP activates protein synthesis through Akt/mTOR pathway, while inhibits proteolysis by inactivating the expression of Ubiquitin proteasome system (UPS) and Autophagy.
Risk Constellation Of Hospital Acquired Pressure Injuries In Patients With A Spinal Cord Injury/ Disorder - Focus On Time Since Spinal Cord Injury/ Disorder And Patients’ Age
Authors: Scheel-Sailer Anke , Neuhauser Charlotte , Sailer Clara , Najmanova Klara , Baumberger Michael , Paez-Granados Diego , Schaefer Dirk , Reto Wettstein 
Published: December 2022

Abstract
Study Design: Prospective cohort study.
Objectives: The aim of this study was to evaluate how time since spinal cord injury/disorder (SCI/D) and patients’ age influence risk constellation for hospital acquired pressure injuries (HAPI) in patients with a SCI/D.
Setting: Acute care and rehabilitation clinic specialized in SCI/D.
Methods: We collected patients’ characteristics and 85 risk factors for HAPI development in adults with SCI/D with at least one HAPI during their inpatient stay between August 2018 and December 2019. We analyzed patients’ characteristics and HAPI risk factors using descriptive statistics according to time since SCI/D (<1 year, 1-15 years, >15 years) and patients’ age (18-35 years, 35-65 years, >65 years).
Result: We identified 182 HAPI in 96 patients. Comparing patients with SCI/D <1 year with the other groups, autonomic dysreflexia (p<0.001), abnormal body temperature (p=0.001), hypertensive episode (p=0.005), and pneumonia (p<0.001) occurred more frequently; mean hemoglobin (p<0.001), albumin (p=0.002) and vitamin D levels (p=0.013) were significantly lower, and patients with time since SCI/D <1 year scored fewer points (10-12) on the Braden Scale (p<0.001). Comparing groups per patients’ age, only the SCIPUS score was higher in patients >65 years compared to the other two groups (p=0.002).
Conclusion: Different risk factor constellation seem to be underlying HAPI development with more differences in patients time since SCI/D than patients’ age. Awareness of these differences in risk factor constellation depending on time since SCI/D in these patients might lead to different HAPI prevention strategies.
Impact of hydrogen gas inhalation during therapeutic hypothermia on cerebral hemodynamics and oxygenation in the asphyxiated piglet
Authors: Nakamura Shinji , Nakao Yasuhiro , Yinmon Htun , Mitsuie Tsutomu , Koyano Kosuke , Morimoto Aya , Konishi Yukihiko , Arioka Makoto , Kondo Sonoko , Kato Ikuko , Ohta Kenichi , Yasuda Saneyuki , Miki Takanori , Ueno Masaki , Kusaka Takashi 
Published: December 2022

Abstract
We previously reported the neuroprotective potential of combined hydrogen (H2) gas ventilation therapy and therapeutic hypothermia (TH) by assessing the short-term neurological outcomes and histological findings of 5-day neonatal hypoxic-ischemic (HI) encephalopathy piglets. However, the effects of H2 gas on cerebral circulation and oxygen metabolism and on prognosis were unknown. Here, we used near-infrared time-resolved spectroscopy to compare between combined H2 gas ventilation and TH and TH alone. Piglets were divided into three groups: HI insult with normothermia (NT, n = 10), HI insult with hypothermia (TH, 33.5 ± 0.5°C, n = 8), and HI insult with hypothermia plus H2 ventilation (TH + H2, 2.1–2.7%, n = 8). H2 ventilation and TH were administered and the CBV and ScO2 were recorded for 24 h after the insult. CBV was significantly higher at 24 h after the insult in the TH + H2 group than in the other groups. ScO2 was significantly lower throughout the 24 h after the insult in the TH + H2 group than in the NT group. In conclusion, combined H2 gas ventilation and TH can increase CBV and decrease ScO2, which possibly reflects elevated cerebral blood flow to meet greater oxygen demand for more surviving neurons versus TH alone.
Application of pedicle axis view in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint
Authors: Zhao Zhongzhen , Zheng Guofu , Chu Xiaodong , Wang Shuofan 
Published: December 2022

Abstract
Aim
To investigate the clinical application of pedicle axis view in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint.
Methods
Percutaneous sacroiliac screw fixation was performed in 29 patients with type III fractures of the sacroiliac joint under imaging monitoring by pedicle axis view of the X-ray fluoroscopy (C-arm) after preoperative traction reduction and preoperative preparation. There were 19 males and 10 females, aged from 20 to 75 years old, with a mean age of 42.1 ± 3.4 years.
Results
The operation time was between 44 and 135 minutes, with a mean of 95.5 ± 9.4 minutes; the intraoperative fluoroscopy time was between 15 and 42 seconds, with a mean of 25 ± 4.7 seconds; the intraoperative blood loss was 5–10 ml, with a mean of 7.1 ± 1.3 ml; according to the Matta scoring system, there were 41 cases with excellent outcomes and 4 cases with good outcomes; based on the definition by Neo et al., pedicle screw positions were categorised into 4 grades: grade 0, 41 screws, grade I, 2 screws, grade II, 2 screws, grade III, 0 screws, and grade 0 and I screws were excellent screws, with an excellent rate of 95.6%; Majeed functional score showed excellent in 37 cases and good in 8 cases, with an excellent and good rate of 100%; 29 cases were followed up between 3 and18 months, with a mean of 7.1 ± 1.2 months. All patients achieved anatomical reduction, with accurate screw placement, no bone penetration, no neurovascular injury, and the fractures of patients healed.
Conclusion
Pedicle axis view using fluoroscopy is a convenient and quick fluoroscopy method in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint, with a high success rate, good safety and short fluoroscopy time.
Viral co-infection of oncogenic Human papillomavirus with Epstein–Barr Virus, Human herpesvirus 8 and Herpes Simplex Virus type 2 in malignant cervical cancer
Authors: Sosse Soukayna Alaoui , Tadlaoui Kaoutar Anouar , Benhassou Mustapha , Elkarroumi Mohamed , Elmzibri Mohamed , Ennaji Moulay Mustapha 
Published: December 2022

Abstract
Cervical cancer is considered the fourth most common malignancy and the fifth fatal cancer in women, oncogenic Human papillomavirus are considered a primary cause of development of cervical cancer. It has also been suggested that viral coexistence may also accelerate the progression of cervical lesions to cervical cancer.
This study aims to study the coinfection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 infections in women with cervical cancer with the presence of Human papillomavirus and their correlations with the clinicopathologic characteristics of patients. In this study, 73 samples that tested positive for Human papillomavirus in previous study were used for the detection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 in tumor tissue using Polymerase Chain Reaction techniques, and the clinical relevance was analyzed statistically. Of the 73 samples (48%) were infected with Epstein Bar, (24.65%) infected with Herpesvirus 8 However, none of the cases were infected with Hepes simplex type 2. The frequency of co-infections was 16.43% of cases. No significant association was found between co-infection and other clinicopathologic features. Therefore, these results represent arguments in favor of the role of Epstein Bar and Herpesvirus 8 among Human papillomavirus positive cases, as potential cofactors in cervical carcinogenesis, which could lead us to develop new therapeutics and preventive vaccines.
Identifying the best PCR enzyme for library amplification in NGS
Authors: Quail Michael , Corton Craig , Uphill James , Keane Jacqueline , Gu Yong 
Published: December 2022

AbstractBackground PCR amplification is a necessary step in many next generation sequencing (NGS) library preparation methods[1] [2]. Whilst many PCR enzymes are developed to amplify single targets efficiently, accurately and with specificity, few are developed to meet the challenges imposed by NGS PCR, namely unbiased amplification of a wide range of different sizes and GC content. As a result PCR amplification during NGS library prep often results in bias toward GC neutral and smaller fragments. As NGS has matured, optimised NGS library prep kits and polymerase formulations have emerged and in this study we have tested a wide selection of available enzymes for both short read Illumina library preparation and long fragment amplification ahead of long-read sequencing. Results We tested over 20 different Hi-fidelity PCR enzymes/NGS amplification mixes on a range of Illumina library templates of varying GC content and composition, and find that both yield and genome coverage uniformity characteristics of the commercially available enzymes varied dramatically. Three enzymes Quantabio RepliQa Hifi Toughmix, Watchmaker Library Amplification Hot Start Master Mix (2X) “Equinox” and Takara Ex Premier were found to give a consistent performance, over all genomes, that mirrored closely that observed for PCR free datasets. We also test a range of enzymes for long read sequencing by amplifying size fractionated S. cerevisiae DNA of average size 21.6 and 13.4kb respectively. Conclusion The enzymes of choice for short read (Illumina) library fragment amplification are Quantabio RepliQa Hifi Toughmix, Watchmaker Library Amplification Hot Start Master Mix (2X) “Equinox” and Takara Ex Premier, with RepliQa also being the best performing enzyme from the enzymes tested for long fragment amplification prior to long read sequencing.
Comparative expression analysis of microRNAs targeting GRAS genes induced by osmotic stress in two contrasting wheat genotypes
Authors: Mishra Shefali , Chaudhary Reeti , Sharma Pradeep 
Published: December 2022

Abstract
Background
MicroRNAs (miRNAs) are important nonprotein-coding genes in plants which participate in in almost all biological processes during abiotic and biotic stresses. Understanding how plants respond to various environmental conditions requires the identification of stress-related miRNAs, and recent years there has been an increased interest in studying miRNA genes and miRNA gene expression. Drought is one of the common environmental stresses limiting plant growth and development. To understand the role of miRNAs in response to osmotic stress, stress-specific miRNAs and their GRAS gene targets were validated.
Results
In this study, we analysed comparative expression patterns of the ten stress-responsive miRNAs and targeting GRAS genes involved in osmotic stress adaptation in order to understand the regulation behaviour of abiotic stress and miRNAs in two contrasting wheat genotype C-306 (drought tolerant) and WL-711 (drought sensitive). Three miRNAs were found to be up-regulated in response to stress, whereas seven miRNAs were found to be down-regulated as a result of the study. In contrast to miRNA, it was also discovered that GRAS genes as their targets were up-regulated during osmotic stress. Furthermore, miR397 and its target GRAS53 were shown to be down-regulated at distinct time intervals while being unaffected by osmotic stress. Variation in the level of expression of studied miRNAs in the presence of target genes provides a likely explanation of miRNA-based abiotic stress regulation. A regulatory network of miRNA and their targets revealed that fourteen miRNA interact with 55 GRAS targets from various subfamilies that contribute in the plant growth and development.
Conclusions
These results provide evidence for temporal and variety-specific differential regulation of miRNAs and their targets in wheat in response to osmotic stress and could help in defining the potential roles of miRNAs in plant adaptation to osmotic stress in future.
Knowledge, attitude, and practice of oxygen administration among nurses working in University of Gondar Comprehensive Specialized Hospital, North Gondar, Northwest Ethiopia, 2022.
Authors: Abitew Kennean 
Published: December 2022

AbstractThe concentration of oxygen in ambient air is 21.00%. It is one of the World Health Organization's lists of essential medications (WHO). However, a high oxygen concentration may alter the lung and result in oxygen poisoning. Surfactant production is impacted by high oxygen concentrations, which also cause alveoli to collapse. To prevent these hazards, the critical care nurse must be able to monitor oxygen administration and gradually reduce supplementary oxygen. One of the issues associated with inadequate understanding, attitude, and practice about oxygen therapy are still delivering appropriate oxygen administration, despite the fact that it is controllable. To provide high-quality treatment, nurses' skills, attitudes, and practices are crucial since they can significantly contribute to preventing respiratory issues caused by a lack of oxygen while hospitalized. Therefore, the purpose of this study was to evaluate nurses' practices, knowledge, and attitudes toward the administration of oxygen in 2022 at the University of Gondar Comprehensive Specialized Hospital in Northwest Ethiopia.Methods: Institutional based cross-sectional study was conducted among nurses working at the University of Gondar comprehensive specialized hospital from May 1 to August 5/2022. A simple random sampling method was used to select study participants and data was collected by presenting self-administrated questionnaires. The data were entered into Epi data version 4.6 and exported into SPSS version 22. Finally, summary statistics were used to describe the study population.Result: A total of 244 respondents have participated with a response rate of 100%. Of the total respondents, 55.3% of participants had good knowledge, 56.1% of study participants had favorable attitudes and 62.7% of participants scored good practice.Conclusion and Recommendation: More than half of the nurses were found to have adequate knowledge, attitude, and practice about oxygen administration. However, it is considerable and not enough. To enhance the knowledge, attitude, and practice of the nurses on oxygen administration more than the current status, the implementation of a comprehensive, systematic, and continuous educational program was recommended.
Production of virus-like particles of porcine circovirus type 2 in baculovirus expression system and its application for antibody detection
Authors: Li Yanwei , Yu Pingping , Bao Yaxuan , Ren Yuwen , Zhao Shaowei , Zhang Xuexian 
Published: December 2022

Abstract
Backgroud
Porcine circovirus type 2 (PCV-2) infection is a growing and persistent threat to the swine industry, and thus the development of serological detection methods for PCV2 is of great necessity for clinical diagnosis, as well as epidemiological investigations. The study aimed to build an indirect enzyme-linked immunosorbent assay (ELISA) to examine antibodies against PCV2 based on virus-like particle (VLP).
Results
Through transmission electron microscopy (TEM), the VLPs were morphologically similar to authentic PCV-2 viruses. Purified VLPs can be detected in immunoblots with PCV-2 antisera, and a predominant protein of approximately 30 kDa was determined by Western blot. The VLPs were shown to have good immunogenicity in mice and stimulated a high level of PCV2-specific antibody titers. The indirect ELISA can detect PCV2 antibody responses in animals had a diagnostic sensitivity and specificity of 98.33% and 93.33% compared to immunofluorescence assay (IFA), respectively. The intra-assay and inter-assay coefficient variations (CVs) within a plate was <10%, and the CV of different ELISA plates was <15%, indicating good repeatability. There was no cross-reaction of this ELISA with antisera against other porcine viruses. A total of 170 serum samples collected from different pig farms in China were tested for anti-PCV2 antibodies, and 151 (88.9%) of the 170 samples were PCV2 antibody positive.
Conclusion
Our findings suggest that this ELISA assay was rapid, specific, and reproducible and can be used for large-scale serological investigations of PCV2 antibodies in pigs.
Polar Photospheric Magnetic Field Evolution and Global Flux Transport
Authors: Petrie Gordon 
Published: December 2022

Abstract
The Sun's polar magnetic fields are of paramount importance in structuring the heliosphere and in seeding the activity cycle, but they are difficult to observe from the ecliptic plane. However, they are formed by observable active-region magnetic flux decay and transport processes. We give an observational description of photospheric flux transport and polar field evolution using the $>40$~yr series (1974-2017) of full-disk line-of-sight photospheric magnetograms from the NSO Kitt Peak Vacuum Telescope (KPVT) 512-channel and Spectromagnetograph (SPMG) and the Synoptic Optical Long-term Investigation of the Sun (SOLIS) Vector Spectro-Magnetograph (VSM). An analytical technique introduced by \citet{Durrantetal04} is adapted to analyze time series of full-disk magnetograms to estimate global photospheric flux transport self-consistently. The major hemispheric flux changes, due to cancellation or transport of leading-polarity flux across the equator, occur around activity maximum each cycle when peak levels of flux emerge $\ge 10^{\circ}$ from the equator. This highlights the efficiency of flux cancellation and transport within each hemisphere, allowing peak levels of leading-polarity flux to reach the equator at this time, letting peak trailing-polarity flux surge poleward. Polar flux evolution follows the hemispheric flux closely each cycle, but with temporal variations dampened by lower-latitude flux cancellation. Cycle 24 was distinguished by large hemispheric flux fluctuations forming a three-part structure: (1) dominant northern activity and north polar flux decrease toward zero; (2) dominant southern activity peak, swift south polar field decrease, polarity reversal, and growth while the north pole was stalled; and (3) southern activity decline, north again dominant, north polar flux growth with reversed polarity. Recent global photospheric field evolution and polar field changes are described using Global Oscillations Network Group (GONG, 2006-present) and Solar Dynamics Observatory Helioseismic and Magnetic Imager (SDO/HMI) line-of-sight magnetograms.
Role of cerebro-uterine ratio in late fetal growth restriction and in hypertensive disorders of pregnancy: A prospective observational study
Authors: Tyagi Shakun , Agarwal Ishita , Naik Sunita , Mala YM 
Published: December 2022

Abstract

Objective: To evaluate the role Cerebro-uterine ratio (CUR) for prediction of adverse perinatal outcomes in pregnancies with late Fetal Growth Restriction (FGR) and Hypertensive Disorders of Pregnancy (HDP)
Study design: Fifty women with FGR, fifty with HDP and 100 normal pregnancies between 34-38 weeks’ gestation were evaluated at a tertiary care centre by Ultrasound Doppler, CPR and CUR were determined and correlated with perinatal outcome.
Results: UtA S/D and PI were significantly higher in cases as compared to controls in both groups (p<0.05). Only CUR was significantly lower in HDP cases compared to controls (p<0.001). CUR showed a higher influence on the occurrence of adverse perinatal outcomes in both groups compared to CPR.
Conclusion: Uterine Artery Doppler is an important predictor of uteroplacental insufficiency. CUR can be a better predictor of adverse perinatal outcomes as compared to CPR in pregnancies complicated by HDP with or without FGR.
An iterative method to solve the nonlinear matrix equation $X^p =A+\sum\limits_{i = 1}^m {M_i^*(B+X^{-1})^{-1}{M_i}} $
Authors: Yu Siting , Peng Jingjing , Li Rui , Tang Zengao , Peng Zhenyun 
Published: December 2022

Abstract
Note: Please see pdf for full title and abstract with equations.
Nonlinear matrix equations $X^p =A+\sum\limits_{i = 1}^m {M_i^*(B+X^{-1})^{-1}{M_i}} $ has extensive applications in control theory, ladder networks, dynamic programming and stochastic filtering. In this paper a fixed point acceleration iteration algorithm is proposed and, based on the basic characteristics of the Thompson distance, the convergence and error estimator of the proposed algorithm are proved. Numerical comparison experiments show that the proposed algorithm is feasible and effective.
Mathematics Subject Classification (2010): 15A24 , 65F30.
Zimmermanne Laband syndrome and infantile systemic hyalinosis: an enigma with two separate terms with overlapping features: case report and literature review
Authors: Owlia Fatemeh , Navabazam Alireza , Akhavan-Karbasi Mohammad-Hasan , Barjoei Mohammad Moein Derakhshan 
Published: December 2022

Abstract

Background Zimmermann-Laband Syndrome (ZLS) and infantile systemic hyalinosis (ISH) are extremely rare genetic disorders characterized by various spectrum manifestations. In spite of other case reports, this case has been reported by oral medicine specialists and oral and maxillofacial surgeons with overlapping features of both syndromes. Case presentation In this study, we reported a 2-year-old female patient with gingival growth and brought about embedded erupted teeth in both jaws. The new aspect of this case report attributed to multiple papulo-nodular skin lesions barely reported by a kind of literature. Surgical excision was performed under general anesthesia. At six months of follow-up after surgery, mastication, and breathing problems were significantly improved. Aesthetic in terms of gingival appearance was ameliorated. Conclusions Although both syndromes remain an enigma for specialists to date because of their ambiguous presentations, the timely diagnosis could be crucial for prognosis and prevention of further serious surcharge. Dentists could have an important role in the diagnosis of rare disorders.