bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic and proteomic studies of condylar ossification of the temporomandibular joint in porcine embryos
Authors: Xiang Lei , Li Yongfeng , Wang Xuewen , Liu HuaWei , Chang Ping , Mu Xiaodan , Tianteng Tengyue , Hu Min 
Published: June 2023

AbstractBackgroundThe ossification mechanism of the temporomandibular joint (TMJ) condyle remains unclear in human embryo. The size and structure of TMJ, shape of articular disc and the characteristics of omnivorous chewing in the pig are similar to those of humans. The pig is an ideal animal for studying the mechanism of ossification of the TMJ condyle during the embryonic period.MethodIn a previous study by our group, it was found that there was no condylar ossification on embryonic day(E) 45, but the ossification of condyle occurred between E75 and E90. In this study, a total of 12 miniature pig embryos on E45 and E85 were used. Six embryos were used for tissue sections (3 in each group). The remaining six embryos were used for transcriptomic and proteomic studies to find differential genes and proteins. The differentially expressed genes in transcriptome and proteomic analysis were verified by QPCR.ResultsIn total, 1592 differential genes comprising 1086 up‐regulated genes and 506 down‐regulated genes were screened for fold changes of ≥2 to ≤0.5 between E45 and E85. In the total of 4613 proteins detected by proteomic analysis, there were 419 differential proteins including 313 up‐regulated proteins and 106 down‐regulated proteins screened for fold changes of ≥2 to ≤0.5 between E45 and E85. A total of 36 differential genes differing in both transcriptome and proteome analysis were found. QPCR analysis showed that 14 of 15 selected genes were consistent with transcriptome analysis.ConclusionCondylar transcriptome and proteomic analysis during the development of TMJ in miniature pigs revealed the regulatory genes/proteins of condylar ossification.
CuCoO2 Nanoparticles as a Nanoprotease for Selective Proteolysis with High Efficiency at Room Temperature
Authors: Xu Jiahao , Ji Ningning , Guo Mingxiu , Wang Yaru , Xu Xiaolong 
Published: June 2023

AbstractMany nanoproteases contain tetravalent metal ions and catalyze peptide‐bond hydrolysis only at high temperature (60 °C). Here, we report a new and effective strategy to explore nanoproteases from nanoparticles containing low valent metal ions. We found that flower‐like CuCoO2 nanoparticles (CuCoO2 NPs) containing low valent Cu+ possessed excellent catalytic activity towards selective cleavage of peptide bonds with hydrophobic residues in bovine serum albumin (BSA) at room temperature. CuCoO2 NPs exhibited excellent stability and had great reusability. CuCoO2 NPs also hydrolyzed heat‐denatured and surfactant‐denatured BSA. Mechanism analysis revealed that the high Lewis acidity of Co3+ and the low valence of Cu+ were both essential for the high protease activity of CuCoO2 NPs. The flower‐like structure of CuCoO2 NPs and the strong nucleophilicity of Cu+‐bound hydroxyl endow them with excellent catalytic performance. The findings open a new way for the design and discovery of high‐efficiency nanoproteases.
Active Ensembles in Methane Dehydroaromatization over Molybdenum/ZSM‐5 Zeolite Identified by 2D 1H−95Mo Magic Angle Spinning Nuclear Magnetic Resonance Correlation Spectroscopy
Authors: Gao Wei , Wang Qiang , Qi Guodong , Liang Jiawei , Wang Chao , Xu Jun , Deng Feng 
Published: June 2023

AbstractMethane dehydroaromatization (MDA) over Mo‐modified zeolite is a potential catalytic route for converting natural gas into valuable aromatics. However, the active species in this reaction are highly complex, involving diverse Mo species, acidic sites of zeolite, and organic molecules. Herein, we apply 1D 95Mo NMR and 2D 1H‐95Mo heteronuclear correlation solid‐state NMR spectroscopy to directly observe the active ensembles in the confined channels of Mo/ZSM‐5 zeolite during the MDA reaction. We monitor the evolution of the spatial correlations of Mo species with the Brønsted acid sites and organic products (olefins and aromatics) in the zeolite channels. We identified two kinds of MoOxCy species, with the more carbidic one (MoOxCy‐II) exhibiting higher activity for methane activation and benzene formation. The strong spatial interactions between the active Mo species and the organic species in the Mo/ZSM‐5 pores are related to the MDA activity.
Ionic Liquid Templated Ordered Hexagonal Mesoporous Iron Phosphate Molecular Sieves: A Highly Effective Heterogeneous Catalysts with Remarkable Selectivity for Phenol Hydroxylation Reaction
Authors: Kumar Maddila Anil , Selvam Parasuraman 
Published: June 2023

AbstractA highly organized hexagonal mesoporous iron phosphate framework structure with the designation HMI‐41 was successfully synthesized for the first‐time in a reproducible way using imidazolium‐based ionic liquid as structure directing agent. The unique templating properties of ionic liquid generated a highly ordered well‐crystallized mesoporous matrix having high surface area (445 m2 g−1), thicker pore wall (2.1 nm) and narrow pore size distribution (3.1 nm). The presence of active sites within a tetrahedral framework structure made the novel HMI‐41 catalyst highly effective for phenol hydroxylation in an acidic medium with hydrogen peroxide as the oxidant. The catalyst exhibited outstanding performance, achieving an impressive 80% hydroquinone selectivity and 21% phenol conversion with a hydroquinone‐to‐catechol ratio of seven, which is the highest value ever reported.
Effectiveness of self‐run vs. third‐party sales channels on the consumers' purchase intention: A multi‐method exploration of the moderating role of review volume
Authors: Song Lijuan , Mo Zan , He Yue , Liu Jianhua , Fu Huijian 
Published: June 2023

AbstractIn practice, many prominent electronic retailers (e‐tailers) offer consumers both self‐run (i.e., reselling mode) and third‐party (i.e., agency selling mode) sales channels. However, previous research has not explained how consumers choose between these two channels and the potential moderating factors of that choice. To narrow this gap in the literature, we conducted a cognitive neuroscience‐based experiment and a scenario‐based experiment to investigate how the consumers' purchase intention is impacted by e‐commerce sales channels (self‐run vs. third‐party) and review volume (low vs. high). As per the results of the experiments, the participants were more likely to buy from the self‐run (vs. third‐party) channel when the review volume was low; however, they did not differ in their purchase intention between the self‐run and third‐party channels when the review volume was high (Studies 1 and 2). The neuroscience data yielded similar findings: When the review volume was low, the self‐run channel elicited more pronounced electroencephalography activity regarding the late positive potential (LPP) than the third‐party channel; when the review volume was high, there was no difference in the LPP induced by the two sales channels (Study 1). In addition, the interaction effect between sales channel and review volume on purchase intention was mediated by perceived product quality (Study 2). Based on these findings, we discussed the theoretical and practical implications of the research.
Airlift Photobioreactor for Microalgae Growth from Emissions: Mathematical Modeling and Simulation
Authors: Rampi Murilo Gasparin , Balmant Wellington , Corrêa Diego de Oliveira , Santos Beatriz , Selesu Nelson Fernando Herculano , Severo Ihana Aguiar , Mariano André Bellin , Vargas José Viriato Coelho , Ordonez Juan C. 
Published: June 2023

AbstractA mathematical model is proposed to describe and simulate microalgae growth from emissions in an outdoor airlift photobioreactor. To this end, the influences of the concentration of total nitrogen, total phosphorus, CO2 present in the exhaust gases, temperature, and light intensity were considered in biomass production. The model was tested under different experimental conditions with biomass concentrations ranging from 2 g L−1 to 4.5 g L−1. Furthermore, the accuracy of the airlift photobioreactor simulations was greater than 90 %. Thus, the developed model was able to satisfactorily describe the experimental data, so that it can be applied in other microalgae systems for optimizing biomass production, both on laboratory and pilot scales.
Investigating Design Rules for Photoinduced Electron Transfer Quenching in Triangulenium Probes
Authors: Dahl Jensen Jesper , Jakobsen Rasmus K. , Yao Zehan , Laursen Bo W. 
Published: June 2023

AbstractFluorescent probes based on photoinduced electron transfer (PET) quenching of long lifetime triangulenium fluorophores have found multiple applications. For such probes a successful design relies on the right balance between the rate of PET quenching and fluorescence. In a series of ADOTA (A) and DAOTA (D) triangulenium fluorophores appended with aniline‐like quencher moieties, we have investigated the rate of quenching and its relation to thermodynamic driving force, distance, and conjugation within the quencher moiety. Three different quenchers, a short (1), a long (2), and a long twisted (3), 4‐aminophenyl, 4’‐aminobiphenyl, and 2,2’‐dimethyl‐4’‐aminobiphenyl, respectively were investigated. Steady‐state spectroscopy and electrochemistry confirms that the quencher moieties are electronically decoupled from the dyes and have similar oxidation potentials and thus driving force for PET quenching, irrespectively of their different length and conjugation. Time‐resolved fluorescence measurement was used to measure the fast PET quenching, with rate constant kPET ranging from >4×1011 to 2×109 s−1. Interestingly, PET quenching is equally efficient/fast from 1 and 2, even with increase in distance between the donor and the acceptor. However, when twisting the biphenyl in 3, a 20‐fold decrease in quenching is found. Even with this decrease in kPET, the quenching in 3 A/D is still highly efficient, with nearly 99 % quenching. The study show that long lifetime fluorophores can be efficiently switched even by relatively slow PET processes and that PET quencher moieties can be removed far from the fluorophore if conjugated linkers are applied.
Natural Light‐Regulated Switchable Self‐Adhesive Supramolecular Films for Smart Windows
Authors: Luo Sha , Zheng Bo , Dong Shengyi 
Published: June 2023

AbstractSwitchable self‐adhesive films are promising coating materials for constructing smart windows. Compared to commonly used hydrogels, self‐adhesive supramolecular films have a variety of advantages and serve as a universal carrier of stimuli‐responsiveness. In this study, supramolecular adhesive material is processed into self‐adhesive films, which display tough and long‐term stable adhesion to commercially available organic/inorganic glass windows. Spiropyrans are used as the photo‐responsive units to endow smart windows with switchable optical behavior. Reverse photochromism is successfully realized by the combination of supramolecular films and spiropyrans. Reversible and rapid transformations in the color and transmittance of smart windows are fully achieved by natural light irradiation in different weather conditions, including sun, rain, and clouds.
Catalysis by KDM6 Histone Demethylases – A Synergy between the Non‐Heme Iron(II) Center, Second Coordination Sphere, and Long‐Range Interactions
Authors: Rifayee Simahudeen Bathir Jaber Sathik , Chaturvedi Shobhit S. , Warner Cait , Wildey Jon , White Walter , Thompson Martin , Schofield Christopher J. , Christov Christo Z. 
Published: June 2023

AbstractKDM6A (UTX) and KDM6B (JMJD3) are human non‐heme Fe(II) and 2‐oxoglutarate (2OG) dependent JmjC oxygenases that catalyze the demethylation of trimethylated lysine 27 in the N‐terminal tail of histone H3, a post‐translational modification that regulates transcription. A Combined Quantum Mechanics/ Molecular Mechanics (QM/MM) and Molecular Dynamics (MD) study on the catalytic mechanism of KDM6A/B reveals that the transition state for the rate‐limiting hydrogen atom transfer (HAT) reaction in KDM6A catalysis is stabilized by polar (Asn217) and aromatic (Trp369)/non‐polar (Pro274) residues in contrast to KDM4, KDM6B and KDM7 demethylases where charged residues (Glu, Arg, Asp) are involved. KDM6A employs both σ‐ and π‐electron transfer pathways for HAT, whereas KDM6B employs the σ‐electron pathway. Differences in hydrogen bonding of the Fe‐chelating Glu252(KDM6B) contribute to the lower energy barriers in KDM6B vs. KDM6A. The study reveals a dependence of the activation barrier of the rebound hydroxylation on the Fe−O−C angle in the transition state of KDM6A. Anti‐correlation of the Zn‐binding domain with the active site residues is a key factor distinguishing KDM6A/B from KDM7/4s. The results reveal the importance of communication between the Fe center, second coordination sphere, and long‐range interactions in catalysis by KDMs and, by implication, other 2OG oxygenases.
The Role of Conjugation in the Halogen−Lithium Exchange Selectivity: Lithiation of 4,6,7,9‐Tetrabromo‐1,3‐dimethyl‐2,3‐dihydro‐1H‐perimidine
Authors: Yakubenko Artyom A. , Tupikina Elena Yu. , Antonov Alexander S. 
Published: June 2023

AbstractThe first case of successful suppression of the coordination of a lithium atom with a dialkylamino group by the effective conjugation of the latter with the aromatic core has been discovered. This effect controls regioselectivity of the bromine−lithium exchange in 4,6,7,9‐tetrabromo‐1,3‐dimethyl‐2,3‐dihydro‐1H‐perimidine, which leads to products with the most effective conjugation. As a result, the product of this quadruple exchange demonstrates no tendency of the coordination of the NMe groups to neighboring lithium atoms despite the absence of steric restrictions. Experimental results are explained by means of quantum chemical calculations: geometry optimization, natural bond analysis and scans using the modredundant scheme.
Influence of Molecular Characteristics and Size Effects in Polystyrene Pyrolysis
Authors: Zimmermann Jana , Mansouri Somayeh , Nobis Christian , Irmer Michael , Beuermann Sabine , Fischlschweiger Michael 
Published: June 2023

AbstractUnderstanding the structure‐property‐process relationships in pyrolysis‐based recycling of polymers offers new possibilities, like, energy‐efficient process designs and tailored products. With the consideration of sample size as well as thermal rate effects, the influence of the molecular weight distribution of polystyrene on the pyrolysis kinetics is investigated. It is shown that molecular weight distribution significantly impacts pyrolysis kinetics. Thermal rate and sample weight affect the material conversion rate and are decisive for determining the thermal processing window.
An AIE Star Polymer with Enhanced Co‐Delivery of Drug and Gene for Synergistic Pest Control†
Authors: Zhou Bingcheng , Jiang Qinhong , Li Jie , Yan Shuo , Shen Jie , Liu Lianying , Yin Meizhen 
Published: June 2023

Comprehensive SummaryPesticides, as the most common means of pest managements, have caused a series of problems such as pest resistance and environmental pollution. Aggregation‐induced emission (AIE) polymers exhibit great potential in biological applications because their fluorescent intensities significantly enhance in the aggregated state. In this paper, an AIE star polymer including a tetraphenyl ethylene (TPE) core and poly(2‐(dimethylamino)ethyl methacrylate) arms is designed and further developed as a multi‐functional nanocarrier for agricultural pest control. The nanocarrier shows high water solubility, good size stability and AIE imaging ability. Meanwhile, the twisted AIE core and positively charged polymer arms make the nanocarrier efficiently co‐load dinotefuran (DIN) and dsRNA via hydrogen bonds and electrostatic interactions, respectively. The AIE star polymer displays low toxicity and high fluorescence traceability. As a result, the nanocarrier co‐loading with DIN and dsRNA exhibits a highly synergistic insecticidal effect with higher pest mortality compared to the separate delivery of DIN and dsRNA. This study develops the AIE star polymer to improve the efficiency of co‐delivery of drug and dsRNA and proposes a new strategy toward efficient and synergistic pest control.
Interleukin‐33 increases type 2 innate lymphoid cell count and their activation in eosinophilic asthma
Authors: Sun Fengfei , Zou Wei , Shi Honglei , Chen Zehu , Ma Donghai , Lin Minmin , Wang Kongqiu , Huang Yiying , Zheng Xiaobin , Tan Cuiyan , Chen Meizhu , Tu Changli , Wang Zhenguo , Wu Jian , Wu Weiming , Liu Jing 
Published: June 2023

AbstractBackgroundInterleukin‐33 (IL‐33) exacerbates asthma probably through type 2 innate lymphoid cells (ILC2s). Nevertheless, the association between eosinophilic asthma (EA) and ILC2s remains obscure, and the mechanisms by which IL‐33 affects ILC2s are yet to be clarified.MethodsILC2s were evaluated in peripheral blood mononuclear cells, induced sputum, and bronchoalveolar lavage fluid obtained from patients with EA. Confocal microscopy was performed to locate ILC2s in lung tissue and the mRNA expression of ILC2‐related genes was also evaluated in the EA model. The proliferation of ILC2s isolated from humans and mice was assessed following IL‐33 or anti‐IL‐33 stimulation.ResultsThe counts, activation, and mRNA expression of relevant genes in ILC2s were higher in PBMCs and airways of patients with EA. In addition, ILC2 cell counts correlated with Asthma control test, blood eosinophil count, Fractional exhaled nitric oxide level, and predicted eosinophilic airway inflammation. IL‐33 induced stronger proliferation of ILC2s and increased their density around blood vessels in the lungs of mice with EA. Moreover, IL‐33 treatment increased the counts and activation of ILC2s and lung inflammatory scores, whereas anti‐IL‐33 antibody significantly reversed these effects in EA mice. Finally, IL‐33 enhanced PI3K and AKT protein expression in ILC2s, whereas inhibition of the PI3K/AKT pathway decreased IL‐5 and IL‐13 production by ILC2s in EA.ConclusionsILC2s, especially activated ILC2s, might be critical markers of EA. IL‐33 can induce and activate ILC2s in the lungs via the PI3K/AKT pathway in EA. Thus, using anti‐IL‐33 antibody could be a part of an effective treatment strategy for EA.
A parallel game model‐based intrusion response system for cross‐layer security in industrial internet of things
Authors: Yu Siyang , Wu Fan , Chen Baoding , Cao Ronghui , Yang Zhibang , Li Keqin 
Published: June 2023

SummaryWith the rise of industrialization, the importance of the industrial Internet of Things (IIoT) has increased significantly, and with it comes a variety of security threats. Therefore, the security of these networks is critical. Industrial Response Systems (IRSs), as the last line of security, plays an important role in the security system of the Industrial Internet of Things. In this paper, a new IRS model based on the non‐cooperative game is proposed. First, by combining the Partially Observable Markov Decision Process (POMDP) model with the stochastic game model based on the expanded attack tree, our model could effectively perceive the changes at each node. Second, our model incorporates the alarms of intrusion detection system (IDS) and the physical quantities of sensors in Industrial Cyber‐Physical System (ICPS) into the quantization system so that the model can respond to intruders more accurately and comprehensively. Finally, we develop this model based on multiprocessors to speed up the solution process, and adopt an approximation algorithm to reduce the number of iterations of the POMDP
Impact of macroscopic on‐site evaluation on the diagnostic outcomes of endoscopic ultrasound‐guided fine‐needle aspiration
Authors: Giri Suprabhat , Uppin Megha S. , Kumar Lohith , Uppin Shantveer , Pamu Pramod Kumar , Angadi Sumaswi , Bhrugumalla Sukanya 
Published: June 2023

AbstractBackgroundEndoscopic ultrasound (EUS)‐guided tissue acquisition is the preferred modality for diagnosing pancreatic lesions and mediastinal and abdominal lymph nodes. Rapid on‐site cytologic evaluation improves the diagnostic outcome of EUS‐guided fine‐needle aspiration (FNA) but is unavailable at many centers. Alternatively, macroscopic on‐site evaluation (MOSE) may improve the diagnostic outcome of EUS‐FNA, but data are limited. Hence, the present study was conducted to assess the efficacy of MOSE in improving adequacy and accuracy.MethodsWe retrospectively analyzed data of consecutive patients with pancreatic or lymph nodal lesions undergoing EUS‐guided FNA at a tertiary care center from December 2020 to December 2022. The study's primary outcomes were adequacy and diagnostic accuracy of the EUS‐guided tissue acquisition, with secondary analysis of predictors of adequacy and accuracy.ResultsData from 124 patients (44.4% male, median age: 54 years) who underwent EUS‐FNA were included in the present analysis. The presence of macroscopic visible core (MVC) on MOSE was reported in 93/124 (75%) cases. An adequate sample for histopathological or cytological examination was obtained in 110/124 (88.7%) cases, while the diagnostic accuracy was 85.5%. On multivariate analysis, the absence of MVC on MOSE was found to be the independent negative predictor of both adequacy (OR 0.092, 95% CI: 0.024–0.349) and accuracy (OR 0.175, 95% CI: 0.057–0.536).ConclusionThe presence of MVC on MOSE can be an indicator of specimen adequacy and can improve the diagnostic yield of EUS‐FNA.
An automated workflow to assess completeness and curate GenBank for environmental DNA metabarcoding: The marine fish assemblage as case study
Authors: Claver Cristina , Canals Oriol , de Amézaga Leire G. , Mendibil Iñaki , Rodriguez‐Ezpeleta Naiara 
Published: June 2023

AbstractTo successfully implement environmental DNA‐based (eDNA) diversity monitoring, the completeness and accuracy of reference databases used for taxonomic assignment of eDNA sequences are among the challenges to be tackled. Here, we have developed a workflow that evaluates the current status of GenBank for marine fishes. For a given combination of species and barcodes, a gap analysis is performed and potentially erroneous sequences are identified. Our gap analysis based on the four most used genes (cytochrome c oxidase subunit 1, 12S rRNA, 16S rRNA, and cytochrome b) for fish eDNA metabarcoding found that COI, the universal choice for metazoans, is the gene covering the highest number of Northeast Atlantic marine fishes (70%), while 12S rRNA, the preferred region for fish‐targeting studies, only covers about 50% of the species. The presence of too close and too distant barcode sequences as expected by their taxonomic classification confirms the existence of erroneous sequences in GenBank that our workflow can detect and eliminate. Comparing taxonomic assignments of real marine eDNA samples with raw and clean reference databases for the most used 12S rRNA barcodes (teleo and MiFish), we confirmed that both barcodes perform differently and demonstrated that the application of the database cleaning workflow can result in drastic changes in community composition. Besides providing a tool for reference database curation, this study confirms the need to increase 12S rRNA reference sequences for European marine fishes and evidences the dangers of taxonomic assignments by directly querying GenBank. We have developed a workflow that evaluates the current status of GenBank for marine fishes. For a given combination of species and barcodes, a gap analysis is performed and potentially erroneous sequences are identified.