bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myeloid-like tumor hybrid cells in bone marrow promote progression of prostate cancer bone metastasis
Authors: Ye Xinyu , Huang Xin , Fu Xing , Zhang Xiao , Lin Risheng , Zhang Wen , Zhang Jian , Lu Yi 
Published: May 2023

Abstract
Background
Bone metastasis is the leading cause of death in patients with prostate cancer (PCa) and currently has no effective treatment. Disseminated tumor cells in bone marrow often obtain new characteristics to cause therapy resistance and tumor recurrence. Thus, understanding the status of disseminated prostate cancer cells in bone marrow is crucial for developing a new treatment.

Methods
We analyzed the transcriptome of disseminated tumor cells from a single cell RNA-sequencing data of PCa bone metastases. We built a bone metastasis model through caudal artery injection of tumor cells, and sorted the tumor hybrid cells by flow cytometry. We performed multi-omics analysis, including transcriptomic, proteomic and phosphoproteomic analysis, to compare the difference between the tumor hybrid cells and parental cells. In vivo experiments were performed to analyze the tumor growth rate, metastatic and tumorigenic potential, drug and radiation sensitivity in hybrid cells. Single cell RNA-sequencing and CyTOF were performed to analyze the impact of hybrid cells on tumor microenvironment.

Results
Here, we identified a unique cluster of cancer cells in PCa bone metastases, which expressed myeloid cell markers and showed a significant change in pathways related to immune regulation and tumor progression. We found that cell fusion between disseminated tumor cells and bone marrow cells can be source of these myeloid-like tumor cells. Multi-omics showed the pathways related to cell adhesion and proliferation, such as focal adhesion, tight junction, DNA replication, and cell cycle, were most significantly changed in these hybrid cells. In vivo experiment showed hybrid cells had a significantly increased proliferative rate, and metastatic potential. Single cell RNA-sequencing and CyTOF showed tumor-associated neutrophils/monocytes/macrophages were highly enriched in hybrid cells-induced tumor microenvironment with a higher immunosuppressive capacity. Otherwise, the hybrid cells showed an enhanced EMT phenotype with higher tumorigenicity, and were resistant to docetaxel and ferroptosis, but sensitive to radiotherapy.

Conclusion
Taken together, our data demonstrate that spontaneous cell fusion in bone marrow can generate myeloid-like tumor hybrid cells that promote the progression of bone metastasis, and these unique population of disseminated tumor cells can provide a potential therapeutic target for PCa bone metastasis.
Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological damage and carcinogenesis through activating mTOR signaling
Authors: Chen Fanghui , Sheng Le , Zhou Tianci , Yan Li , Loveless Reid , Li Honglin , Teng Yong , Cai Yafei 
Published: May 2023

Abstract
Background
Ufm1-specific ligase 1 (Ufl1) and Ufm1-binding protein 1 (Ufbp1), as putative targets of ubiquitin-fold modifier 1 (Ufm1), have been implicated in several pathogenesis-related signaling pathways. However, little is known about their functional roles in liver disease.

Methods
Hepatocyte-specific Ufl1Δ/Δhep and Ufbp1Δ/Δhep mice were used to study their role in liver injury. Fatty liver disease and liver cancer were induced by high-fat diet (HFD) and diethylnitrosamine (DEN) administration, respectively. iTRAQ analysis was employed to screen for downstream targets affected by Ufbp1 deletion. Co-immunoprecipitation was used to determine the interactions between the Ufl1/Ufbp1 complex and the mTOR/GβL complex.

Results
Ufl1Δ/Δhep or Ufbp1Δ/Δhep mice exhibited hepatocyte apoptosis and mild steatosis at 2 months of age and hepatocellular ballooning, extensive fibrosis, and steatohepatitis at 6–8 months of age. More than 50% of Ufl1Δ/Δhep and Ufbp1Δ/Δhep mice developed spontaneous hepatocellular carcinoma (HCC) by 14 months of age. Moreover, Ufl1Δ/Δhep and Ufbp1Δ/Δhep mice were more susceptible to HFD-induced fatty liver and DEN-induced HCC. Mechanistically, the Ufl1/Ufbp1 complex directly interacts with the mTOR/GβL complex and attenuates mTORC1 activity. Ablation of Ufl1 or Ufbp1 in hepatocytes dissociates them from the mTOR/GβL complex and activates oncogenic mTOR signaling to drive HCC development.

Conclusions
These findings reveal the potential role of Ufl1 and Ufbp1 as gatekeepers to prevent liver fibrosis and subsequent steatohepatitis and HCC development by inhibiting the mTOR pathway.
Efficacy and safety of human umbilical cord-derived mesenchymal stem cells (hUC-MSC PLEB001) for the treatment of grade II–IV steroid-refractory acute graft-versus-host disease: a study protocol for a multicenter, randomized, double-blind, placebo-controlled, phase II trial
Authors: Yang Donglin , Hou Xiaoqiang , Qian Kun , Li Yuhang , Hu Liangding , Li Liang , Han Mingzhe , Yao Chen , Liu Daihong 
Published: May 2023

Abstract
Background
Systemic corticosteroid therapy failure is quite common in patients with newly diagnosed acute graft-versus-host disease (aGVHD). Growing evidence has suggested that mesenchymal stem cell (MSC) therapy could be a promising treatment option for aGVHD due to its distinctive immunomodulating functions. However, there is a lack of randomized well-controlled clinical trials.

Methods
This is a clinical trial protocol for a multicenter, randomized, double-blind, placebo-controlled phase II study. The aim of the trial is to evaluate the efficacy and safety of the administration of the human umbilical cord-derived MSC product hUC-MSC PLEB001 in patients with grade II–IV, steroid-refractory aGVHD. A total of 96 patients will be randomized 1:1 to receive MSC or placebo treatment twice per week for 4 weeks, in addition to second-line therapy according to institutional standards. Patients who achieve partial response (PR) at day 28 will be eligible to receive further infusions twice per week for an additional 4 weeks.

Discussion
This study will evaluate the efficacy and safety of MSC therapy in patients who have failed first-line steroid treatment for grade II–IV aGVHD.

Trial registration
Chinese Clinical Trial Registry (ChiCTR), ChiCTR2000035740. Registered on 16 August 2020.
Impacts of plasma microbial lipopolysaccharide translocation on B cell perturbations and anti-CD4 autoantibody production in people with HIV on suppressive antiretroviral therapy
Authors: Fu Xiaoyu , Cheng Da , Luo Zhenwu , Heath Sonya L. , Adekunle Ruth , McKinnon John E , Martin Lisa , Sheng Zizhang , Espinosa Enrique , Jiang Wei 
Published: May 2023

Abstract
Background
. Up to 20% of people with HIV (PWH) who undergo virologically suppressed antiretroviral therapy (ART) fail to experience complete immune restoration. We recently reported that plasma anti-CD4 IgG (antiCD4IgG) autoantibodies from immune non-responders specifically deplete CD4 + T cells via antibody-dependent cytotoxicity. However, the mechanism of antiCD4IgG production remains unclear.

Methods
. Blood samples were collected from 16 healthy individuals and 25 PWH on suppressive ART. IgG subclass, plasma lipopolysaccharide (LPS), and antiCD4IgG levels were measured by ELISA. Gene profiles in B cells were analyzed by microarray and quantitative PCR. Furthermore, a patient-derived antiCD4IgG–producing B cell line was generated and stimulated with LPS in vitro. B cell IgG class switch recombination (CSR) was evaluated in response to LPS in splenic B cells from C57/B6 mice in vitro.

Results
. Increased plasma anti-CD4 IgGs in PWH were predominantly IgG1 and associated with increased plasma LPS levels as well as B cell expression of TLR2, TLR4, and MyD88 mRNA in vivo. Furthermore, LPS stimulation induced antiCD4IgG production in the antiCD4IgG B cell line in vitro. Finally, LPS promoted CSR in vitro.

Conclusion
. Our findings suggest that persistent LPS translocation may promote anti-CD4 autoreactive B cell activation and antiCD4IgG production in PWH on ART, which may contribute to gradual CD4 + T cell depletion. This study suggests that reversing a compromised mucosal barrier could improve ART outcomes in PWH who fail to experience complete immune restoration.
Sharp inequalities related to the volume of the unit ball in $\mathbb{R}^{n}$
Authors: Han Xue-Feng , Chen Chao-Ping 
Published: May 2023

AbstractLet$\Omega _{n}=\pi ^{n/2}/\Gamma (\frac{n}{2}+1)$Ωn=πn/2/Γ(n2+1)($n \in \mathbb{N}$n∈N) denote the volume of the unit ball in$\mathbb{R}^{n}$Rn. In this paper, the logarithmically complete monotonicity of a function involving the ratio of two gamma functions is presented, which yields a sharp double inequality for the quantity$\Omega _{n}^{2}/(\Omega _{n-1}\Omega _{n+1})$Ωn2/(Ωn−1Ωn+1). Also, we establish new sharp inequalities for the quantity$\Omega _{n}^{2}/(\Omega _{n-1}\Omega _{n+1})$Ωn2/(Ωn−1Ωn+1).
Effect of using 5G and cloud computing environment for independent college english vocabulary learning
Authors: Wang Tiantian 
Published: May 2023

AbstractAn independent university refers to an institution of higher learning that has a bachelor's degree or higher education, and cooperates with social organizations other than state institutions or individuals to use non-state financial funds to establish and implement undergraduate education. While welcoming the arrival of the fifth-generation communication technology (5G) era, China's independent universities are also carrying out educational reforms to adapt to social development. 5G has created new opportunities for the development of students. Cloud computing refers to a system with extremely strong computing power formed through computer networks (mostly the Internet), which can store, collect relevant resources and configure them as needed to provide personalized services to users. Therefore, this paper studied and discussed the application of 5G and cloud computing in English vocabulary learning in independent universities. This paper explores the impact of 5G and cloud computing environments on independent college English vocabulary learning. With the assistance of 5G technology and a cloud computing environment, students' vocabulary mastery and application abilities have achieved certain improvements. Students' vocabulary learning can not only effectively break through the constraints of region and time, but also improve the interaction of learning and promote the communication and exchange between learners. This can not only deepen the understanding of vocabulary but also stimulate students' learning initiative and improve their learning ability and level. Given the current situation of English vocabulary acquisition, this paper used the fuzzy evaluation method to establish an evaluation model. The learning platform built by 5G and cloud computing technology has been utilized to study the effect of applying it to learners' vocabulary learning. In the questionnaire survey, 43.00% of the students are generally satisfied with the current English vocabulary teaching, 33.20% of the students think that their learning effect is average, 43.00% of the students think that the arrangement of teaching content is not reasonable, and 35.40% of the students think that the arrangement of class hours is generally reasonable. Before the experiment, the vocabulary of 68 and 65 students in the experimental group and the control group were between 1500–3500. Through the experiment, 67 students in the experimental group had vocabularies in the range of 3501–6500, while the vast majority of students in the control group still had vocabularies in the range of 1500–3500. In addition, through the experiment, the average total score of English test scores increased from 67 to 87 for the experimental group and from 69 to 72 for the control group. Based on the above data, it can be seen that the learning of English vocabulary through independent learning platforms built on 5G and cloud computing has a significant effect on the learning of English vocabulary of independent university students.
Prognostic value of the early lung ultrasound B-line score for postoperative pulmonary insufficiency in patients undergoing thoracic surgery: an observational study
Authors: He Yipeng , Xu Xiaoxiao , Wang Chenhao , Wu Zhouquan 
Published: May 2023

Abstract
Background
Postoperative pulmonary insufficiency (PPI) is an important contributor to morbidity and mortality after thoracic surgery. Lung ultrasound is a reliable tool for assessing respiratory function. We sought to determine the clinical value of the early lung ultrasound B-line score for predicting changes in pulmonary function after thoracic surgery.

Methods
Eighty-nine patients undergoing elective lung surgery were included in this study. The B-line score was determined 30 min after removal of the endotracheal tube, and the PaO2/FiO2 ratio was recorded 30 min after extubation and on the third postoperative day. Patients were divided into normal (PaO2/FiO2 ≥ 300) and PPI (PaO2/FiO2 < 300) groups according to their PaO2/FiO2 ratios. A multivariate logistic regression model was used to identify independent predictors of postoperative pulmonary insufficiency. Receiver operating characteristic (ROC) analysis was performed for significantly correlated variables.

Results
Eighty-nine patients undergoing elective lung surgery were included in this study. We evaluated 69 patients in the normal group and 20 in the PPI group. Patients conforming to NYHA class 3 at administration were significantly more represented in the PPI group (5.8 and 55%; p < 0.001). B-line scores were significantly higher in the PPI group than in the normal group (16; IQR 13–21 vs. 7; IQR 5–10; p < 0.001). The B-line score was an independent risk factor (OR = 1.349 95% CI 1.154–1.578; p < 0.001), and its best cutoff value for predicting PPI was 12 (sensitivity: 77.5%; specificity: 66.7%).

Conclusions
Lung ultrasound B-line scores 30 min after extubation are effective in predicting early PPI in patients undergoing thoracic surgery.
Trial registration This study was registered with the Chinese Clinical Trials Registry (ChiCTR2000040374).
Functional outcomes and quality of life after surgically treated tibial plateau fractures
Authors: Bagherifard Abolfazl , Mirkamali Seyed Farzam , Rashidi Heeva , Naderi Nima , Hassanzadeh Mohammad , Mohammadpour Mehdi 
Published: May 2023

Abstract
Background
Tibial plateau fractures (TPF) are uncommon and challenging for orthopedic surgeons with controversial reported outcomes. In this study, we aimed to evaluate the functional outcomes and quality of life (QOL) of patients with surgically treated TPF.

Methods
A total of 80 consecutive patients and 82 controls participated in this case control study. The patients were all surgically treated in our tertiary center from April 2012 to April 2020. The functional outcome was evaluated using the Western Ontario and McMaster Universities Arthritis Index (WOMAC) scale. Moreover, we used the Short Form 36 health survey (SF-36) health survey to evaluate the QOL.

Results
No significant difference was observed in the overall mean SF-36 score in the two groups. We found a significant positive correlation between the scores of the SF-36 and WOMAC questionnaires (r = 0.642, p < 0.001) and between the ROM and the WOMAC questionnaire score (r = 0.478, p < 0.001). Further, ROM and SF-36 showed a weak positive correlation (r = 0.248, p = 0.026). Age had a weak negative correlation with the pain subscale of SF-36 (r = − 0.255, p = 0.22), even though it was not correlated with the total score or other subscales (p > 0.05).

Conclusion
QoL after TPF is not significantly different from that of a matched control group. Also, neither age nor BMI correlates with the QoL and functional outcome.
Autoimmune alopecia areata due to thymoma without myasthenia gravis: a case report
Authors: Saito Yukino , Yazawa Tomohiro , Nagashima Toshiteru , Ohtaki Yoichi , Kawatani Natsuko , Narusawa Eiji , Yoshikawa Ryohei , Matsumura Nozomi , Maehara Tatsuro , Shirabe Ken 
Published: May 2023

Abstract
Background
Thymoma is associated with autoimmune diseases. Myasthenia gravis is frequently associated with thymoma, but cases of thymoma complicated by alopecia areata are very rare. In this report, we present a case of thymoma associated with alopecia areata, but without Myasthenia gravis.

Case presentation
A 60-year-old woman had a complaint of rapid progression of alopecia areata. A hair follicular biopsy was performed, which showed the infiltration of CD8-positive lymphocytes. She was prescribed topical steroids for 2 months prior to surgery, but her hair loss was not improved. Screening computed tomography showed a mass in the anterior mediastinum, which was suspected to be a thymoma. Myasthenia gravis was ruled out because she had no relevant symptoms or physical findings, and no anti-acetylcholine receptor antibodies were detected in serum. We performed a transsternal extended thymectomy based on a diagnosis of thymoma Masaoka stage I, without myasthenia gravis. Pathological examination showed Type AB thymoma, Masaoka stage II. The chest drainage tube was removed on postoperative day 1, and the patient was discharged on postoperative day 6. The patient has continued topical steroids and showed improvement 2 months postoperatively.

Conclusions
Although alopecia areata is a rare complication in thymoma cases without myasthenia gravis, thoracic surgeons need to keep this condition in mind because alopecia reduces the patient's quality of life.
Destruction mechanism of organic matter enrichment controlled by tectonism in the lower Silurian organic-rich marine mudstone in the western South China Block
Authors: Sun Tong , Zhu Yiqing , Ran Bo , Liang Ke , Luo Chao , Han Yuyue 
Published: May 2023

This destruction mechanism of organic matter enrichment plays an important role in improving the mudstone sedimentary structure system. For the lower Silurian marine mudstone in the western South China Block, the destruction mechanism of organic matter enrichment is unclear, with previous studies ignoring the control from paleogeography and spatial differences. Lamination is a typical sedimentary structure in mudstone and different types of lamination in mudstones can reflect a range of depositional environments. Lenticular lamination, composed of arranged lenses of variable composition, is a common type of lamination in marine mudstone and mainly occurs in organic-lean mudstone. The origin of lenticular lamination remains controversial, and a multidisciplinary approach is needed that integrates sedimentology, petrography, and paleogeography. The main objectives of this approach are to (1) interpret the formation mechanism of lenticular laminations in the Longmaxi marine mudstone, and (2) discuss the significance of lenticular lamination to organic matter destruction. The results not only allow for a complete understanding of the destruction mechanisms of the Silurian marine mudstones but also provide new insights into the sedimentary processes of marine mudstone.
Factors prompting and deterring suicides on the roads
Authors: Norman Hilary , Marzano Lisa , Winter Rachel , Crivatu Ioana , Mackenzie Jay-Marie , Marsh Ian 
Published: May 2023

Background
In addition to the devastating impact on the individual and their families, suicides on the roads can cause distress and harm to other people who might be involved in a collision or witness an attempt. Despite an increased focus on the characteristics and circumstances of road-related suicides, little is known about why people choose to end their lives in this way.

Aims
The aim of the current study was to investigate the factors prompting and deterring the decision to attempt suicide on the roads.

Method
We conducted a secondary analysis of survey data, as well as seven in-depth qualitative interviews. Participants had lived experience of suicidal ideation or behaviour at a bridge or road location. We also carried out an online ethnography to explore interactions in different online communities relating to this method of suicide.

Results
Participants perceived a road-related suicide to be quick, lethal, easy and accessible and to have the potential to appear accidental. The proportion of participants who described their thoughts and attempts as impulsive appeared to be higher than had been observed with other method choices. The potential impact on other people was a strongly dissuasive factor.

Conclusions
Measures designed to prevent access to potentially lethal sites may be particularly important, given that many participants described their thoughts and behaviour as impulsive. In addition, fostering a culture of care and consideration for other road users may help to dissuade people from taking action on the roads.
A fractionally time-delayed SD-Van der Pol oscillator with a non-perturbative approach
Authors: El-Dib Yusry O. , Elgazery Nasser S. , Alyousef Haifa A. 
Published: May 2023

Abstract
The primary target of the present article is to use a fast and efficient technique to obtain an analytical solution for a Duffing-Van der Pol-SD (D-VDP-SD) oscillator with a time delay. Depending on the smoothness parameter, this kind of vibration behaves in both discontinuous and smooth dynamical systems. This novel technique is presented for transmission from a nonlinear delay fractional oscillator to a linear classical oscillator with ordinary derivatives. The transformation into an equivalent oscillator is described in detail. Based on the comparison, the convergence of the numerical and analytical solutions appears satisfactory, which is an indicator of the accuracy of the solutions produced by the suggested technique.
The Strain of Spanning Structural Holes: How Brokering Leads to Burnout and Abusive Behavior
Authors: Lee Jung Won , Quintane Eric , Lee Sun Young , Ruiz Camila Umaña , Kilduff Martin 
Published: May 2023

Connecting otherwise disconnected individuals and groups—spanning structural holes—can earn social network brokers faster promotions, higher remuneration, and enhanced creativity. Organizations also benefit through improved communication and coordination from these connections between knowledge silos. Neglected in prior research, however, has been theory and evidence concerning the psychological costs to individuals of engaging in brokering activities. We build new theory concerning the extent to which keeping people separated (i.e., tertius separans brokering) relative to bringing people together (i.e., tertius iungens brokering) results in burnout and in abusive behavior toward coworkers. Engagement in tertius separans brokering, relative to tertius iungens brokering, we suggest, burdens people with onerous demands while limiting access to resources necessary to recover. Across three studies, we find that tertius separans leads to abusive behavior of others, mediated by an increased experience of burnout on the part of the broker. First, we conducted a five-month field study of burnout and abusive behavior, with brokering assessed via email exchanges among 1,536 university employees in South America. Second, we examined time-separated data on self-reported brokering behaviors, burnout, and coworker abuse among 242 employees of U.S. organizations. Third, we experimentally investigated the effects of the two types of brokering behaviors on burnout and abusive behavior for 273 employed adults. The results across three studies showed that tertius separans brokering puts the broker at an increased risk of burnout and subsequent abusive behavior toward others in the workplace. Funding: E. Quintane received funding from Ernst & Young GmbH Wirtschaftsprüfungsgesellschaft.