bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge, Attitude, and Associated Factors towards Prehospital Care among Emergency Health Care Providers Working in Selected Prehospital Care Centers in Addis Ababa, Ethiopia: A Cross-Sectional Study
Authors: Mamo Alemayehu Sileshi , Gete Birhanu Chekol , Shiferaw Mehari Alemayehu , Zeleke Yared Getachew 
Published: June 2023

AbstractBackground:Patients’ health outcomes can suffer as a result of poor knowledge and unfavorable attitude towards prehospital emergency care. The purpose of this study was to assess emergency health care providers’ (EHCPs’) knowledge, attitude, and associated factors towards prehospital care in selected prehospital Emergency Medical Service institutions in Addis Abeba, Ethiopia.Methods:An institutional-based cross-sectional study design was conducted among EHCPs working in the three selected prehospital emergency medical care centers in Addis Ababa. Data were collected using a standard self-administered questionnaire, cleaned, coded, and entered into EPI Data Version 6, and then exported to SPSS Version 26 for further analysis. The generated data were compiled using frequency tables, charts, and percentages. Logistic regression analysis was used to see the association between independent and dependent variables.Results:One hundred thirty-five (135) study participants were included in this study, with a response rate of 95.7%. The mean age of the respondents was 29.2 years (SD = 4.86). Almost three-quarters of the respondents (71.1%) were aged between 26 and 35 years. Of the total participants, 58.5% and 62.2% of EHCPs had good knowledge and a favorable attitude towards prehospital care, respectively. The study revealed that profession (AOR = 3.2; 95% CI, 1.03 - 7.65), educational status (AOR = 1.17; 95% CI, 1.08 - 4.93), and having training (AOR = 2.25; 95% CI, 1.33 - 4.52) were significantly associated with the knowledge of EHCPs. This finding also revealed that the respondent’s knowledge (AOR = 1.36; 95% CI, 1.05 - 2.32) and having training (AOR = 3.2; 95% CI, 1.24 - 7.83) were significantly associated with EHCPs’ attitudes towards prehospital care.Conclusions:The knowledge and attitude of EHCPs regarding prehospital care were found to be good and favorable as compared to previous studies. In-service training regarding emergency health conditions and the time needed to care for the patient is important for quality prehospital emergency medical care.
Four-dimensional mapping of dynamic longitudinal brain subcortical development and early learning functions in infants
Authors: Chen Liangjun , Wang Ya , Wu Zhengwang , Shan Yue , Li Tengfei , Hung Sheng-Che , Xing Lei , Zhu Hongtu , Wang Li , Lin Weili , Li Gang 
Published: June 2023

AbstractBrain subcortical structures are paramount in many cognitive functions and their aberrations during infancy are predisposed to various neurodevelopmental and neuropsychiatric disorders, making it highly essential to characterize the early subcortical normative growth patterns. This study investigates the volumetric development and surface area expansion of six subcortical structures and their associations with Mullen scales of early learning by leveraging 513 high-resolution longitudinal MRI scans within the first two postnatal years. Results show that (1) each subcortical structure (except for the amygdala with an approximately linear increase) undergoes rapid nonlinear volumetric growth after birth, which slows down at a structure-specific age with bilaterally similar developmental patterns; (2) Subcortical local area expansion reveals structure-specific and spatiotemporally heterogeneous patterns; (3) Positive associations between thalamus and both receptive and expressive languages and between caudate and putamen and fine motor are revealed. This study advances our understanding of the dynamic early subcortical developmental patterns.
Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling
Authors: Nunes-Santos Cristiane J. , Kuehn HyeSun , Boast Brigette , Hwang SuJin , Kuhns Douglas B. , Stoddard Jennifer , Niemela Julie E. , Fink Danielle L. , Pittaluga Stefania , Abu-Asab Mones , Davies John S. , Barr Valarie A. , Kawai Tomoki , Delmonte Ottavia M. , Bosticardo Marita , Garofalo Mary , Carneiro-Sampaio Magda , Somech Raz , Gharagozlou Mohammad , Parvaneh Nima , Samelson Lawrence E. , Fleisher Thomas A. , Puel Anne , Notarangelo Luigi D. , Boisson Bertrand , Casanova Jean-Laurent , Derfalvi Beata , Rosenzweig Sergio D. 
Published: June 2023

AbstractWe describe the first cases of germline biallelic null mutations in ARPC5, part of the Arp2/3 actin nucleator complex, in two unrelated patients presenting with recurrent and severe infections, early-onset autoimmunity, inflammation, and dysmorphisms. This defect compromises multiple cell lineages and functions, and when protein expression is reestablished in-vitro, the Arp2/3 complex conformation and functions are rescued. As part of the pathophysiological evaluation, we also show that interleukin (IL)−6 signaling is distinctively impacted in this syndrome. Disruption of IL-6 classical but not trans-signaling highlights their differential roles in the disease and offers perspectives for therapeutic molecular targets.
Ultra-fast and accurate electron ionization mass spectrum matching for compound identification with million-scale in-silico library
Authors: Yang Qiong , Ji Hongchao , Xu Zhenbo , Li Yiming , Wang Pingshan , Sun Jinyu , Fan Xiaqiong , Zhang Hailiang , Lu Hongmei , Zhang Zhimin 
Published: June 2023

AbstractSpectrum matching is the most common method for compound identification in mass spectrometry (MS). However, some challenges limit its efficiency, including the coverage of spectral libraries, the accuracy, and the speed of matching. In this study, a million-scale in-silico EI-MS library is established. Furthermore, an ultra-fast and accurate spectrum matching (FastEI) method is proposed to substantially improve accuracy using Word2vec spectral embedding and boost the speed using the hierarchical navigable small-world graph (HNSW). It achieves 80.4% recall@10 accuracy (88.3% with 5 Da mass filter) with a speedup of two orders of magnitude compared with the weighted cosine similarity method (WCS). When FastEI is applied to identify the molecules beyond NIST 2017 library, it achieves 50% recall@1 accuracy. FastEI is packaged as a standalone and user-friendly software for common users with limited computational backgrounds. Overall, FastEI combined with a million-scale in-silico library facilitates compound identification as an accurate and ultra-fast tool.
Nivolumab plus chemoradiotherapy in locally-advanced cervical cancer: the NICOL phase 1 trial
Authors: Rodrigues Manuel , Vanoni Giulia , Loap Pierre , Dubot Coraline , Timperi Eleonora , Minsat Mathieu , Bazire Louis , Durdux Catherine , Fourchotte Virginie , Laas Enora , Pouget Nicolas , Castel-Ajgal Zahra , Marret Gregoire , Lesage Laetitia , Meseure Didier , Vincent-Salomon Anne , Lecompte Lolita , Servant Nicolas , Vacher Sophie , Bieche Ivan , Malhaire Caroline , Huchet Virginie , Champion Laurence , Kamal Maud , Amigorena Sebastian , Lantz Olivier , Chevrier Marion , Romano Emanuela 
Published: June 2023

AbstractConcurrent chemoradiotherapy (CRT) with blockade of the PD-1 pathway may enhance immune-mediated tumor control through increased phagocytosis, cell death, and antigen presentation. The NiCOL phase 1 trial (NCT03298893) is designed to determine the safety/tolerance profile and the recommended phase-II dose of nivolumab with and following concurrent CRT in 16 women with locally advanced cervical cancer. Secondary endpoints include objective response rate (ORR), progression free survival (PFS), disease free survival, and immune correlates of response. Three patients experience grade 3 dose-limiting toxicities. The pre-specified endpoints are met, and overall response rate is 93.8% [95%CI: 69.8–99.8%] with a 2-year PFS of 75% [95% CI: 56.5–99.5%]. Compared to patients with progressive disease (PD), progression-free (PF) subjects show a brisker stromal immune infiltrate, higher proximity of tumor-infiltrating CD3+ T cells to PD-L1+ tumor cells and of FOXP3+ T cells to proliferating CD11c+ myeloid cells. PF show higher baseline levels of PD-1 and ICOS-L on tumor-infiltrating EMRA CD4+ T cells and tumor-associated macrophages, respectively; PD instead, display enhanced PD-L1 expression on TAMs, higher peripheral frequencies of proliferating Tregs at baseline and higher PD-1 levels at week 6 post-treatment initiation on CD4 and CD8 T cell subsets. Concomitant nivolumab plus definitive CRT is safe and associated with encouraging PFS rates. Further validation in the subset of locally advanced cervical cancer displaying pre-existing, adaptive immune activation is warranted.
Bridging multiscale interfaces for developing ionically conductive high-voltage iron sulfate-containing sodium-based battery positive electrodes
Authors: Zhang Jiyu , Yan Yongliang , Wang Xin , Cui Yanyan , Zhang Zhengfeng , Wang Sen , Xie Zhengkun , Yan Pengfei , Chen Weihua 
Published: June 2023

AbstractNon-aqueous sodium-ion batteries (SiBs) are a viable electrochemical energy storage system for grid storage. However, the practical development of SiBs is hindered mainly by the sluggish kinetics and interfacial instability of positive-electrode active materials, such as polyanion-type iron-based sulfates, at high voltage. Here, to circumvent these issues, we proposed the multiscale interface engineering of Na2.26Fe1.87(SO4)3, where bulk heterostructure and exposed crystal plane were tuned to improve the Na-ion storage performance. Physicochemical characterizations and theoretical calculations suggested that the heterostructure of Na6Fe(SO4)4 phase facilitated ionic kinetics by densifying Na-ion migration channels and lowering energy barriers. The (11-2) plane of Na2.26Fe1.87(SO4)3 promoted the adsorption of the electrolyte solution ClO4− anions and fluoroethylene carbonate molecules, which formed an inorganic-rich Na-ion conductive interphase at the positive electrode. When tested in combination with a presodiated FeS/carbon-based negative electrode in laboratory- scale single-layer pouch cell configuration, the Na2.26Fe1.87(SO4)3-based positive electrode enables an initial discharge capacity of about 83.9 mAh g−1, an average cell discharge voltage of 2.35 V and a specific capacity retention of around 97% after 40 cycles at 24 mA g−1 and 25 °C.
Unmodified rabies mRNA vaccine elicits high cross-neutralizing antibody titers and diverse B cell memory responses
Authors: Hellgren Fredrika , Cagigi Alberto , Arcoverde Cerveira Rodrigo , Ols Sebastian , Kern Theresa , Lin Ang , Eriksson Bengt , Dodds Michael G. , Jasny Edith , Schwendt Kim , Freuling Conrad , Müller Thomas , Corcoran Martin , Karlsson Hedestam Gunilla B. , Petsch Benjamin , Loré Karin 
Published: June 2023

AbstractLicensed rabies virus vaccines based on whole inactivated virus are effective in humans. However, there is a lack of detailed investigations of the elicited immune response, and whether responses can be improved using novel vaccine platforms. Here we show that two doses of a lipid nanoparticle-formulated unmodified mRNA vaccine encoding the rabies virus glycoprotein (RABV-G) induces higher levels of RABV-G specific plasmablasts and T cells in blood, and plasma cells in the bone marrow compared to two doses of Rabipur in non-human primates. The mRNA vaccine also generates higher RABV-G binding and neutralizing antibody titers than Rabipur, while the degree of somatic hypermutation and clonal diversity of the response are similar for the two vaccines. The higher overall antibody titers induced by the mRNA vaccine translates into improved cross-neutralization of related lyssavirus strains, suggesting that this platform has potential for the development of a broadly protective vaccine against these viruses.
Non-covalent inhibitors of thioredoxin glutathione reductase with schistosomicidal activity in vivo
Authors: Petukhova Valentina Z. , Aboagye Sammy Y. , Ardini Matteo , Lullo Rachel P. , Fata Francesca , Byrne Margaret E. , Gabriele Federica , Martin Lucy M. , Harding Luke N. M. , Gone Vamshikrishna , Dangi Bikash , Lantvit Daniel D. , Nikolic Dejan , Ippoliti Rodolfo , Effantin Grégory , Ling Wai Li , Johnson Jeremy J. , Thatcher Gregory R. J. , Angelucci Francesco , Williams David L. , Petukhov Pavel A. 
Published: June 2023

AbstractOnly praziquantel is available for treating schistosomiasis, a disease affecting more than 200 million people. Praziquantel-resistant worms have been selected for in the lab and low cure rates from mass drug administration programs suggest that resistance is evolving in the field. Thioredoxin glutathione reductase (TGR) is essential for schistosome survival and a validated drug target. TGR inhibitors identified to date are irreversible and/or covalent inhibitors with unacceptable off-target effects. In this work, we identify noncovalent TGR inhibitors with efficacy against schistosome infections in mice, meeting the criteria for lead progression indicated by WHO. Comparisons with previous in vivo studies with praziquantel suggests that these inhibitors outperform the drug of choice for schistosomiasis against juvenile worms.
The effect of familiarity on behavioral oscillations in face perception
Authors: Liu Xiaoyi , Melcher David 
Published: June 2023

Abstract
Studies on behavioral oscillations demonstrate that visual sensitivity fluctuates over time and visual processing varies periodically, mirroring neural oscillations at the same frequencies. Do these behavioral oscillations reflect fixed and relatively automatic sensory sampling, or top-down processes such as attention or predictive coding? To disentangle these theories, the current study used a dual-target rapid serial visual presentation paradigm, where participants indicated the gender of a face target embedded in streams of distractors presented at 30 Hz. On critical trials, two identical targets were presented with varied stimulus onset asynchrony from 200 to 833 ms. The target was either familiar or unfamiliar faces, divided into different blocks. We found a 4.6 Hz phase-coherent fluctuation in gender discrimination performance across both trial types, consistent with previous reports. In addition, however, we found an effect at the alpha frequency, with behavioral oscillations in the familiar blocks characterized by a faster high-alpha peak than for the unfamiliar face blocks. These results are consistent with the combination of both a relatively stable modulation in the theta band and faster modulation of the alpha oscillations. Therefore, the overall pattern of perceptual sampling in visual perception may depend, at least in part, on task demands.

Protocol registration
The stage 1 protocol for this Registered Report was accepted in principle on 16/08/2022. The protocol, as accepted by the journal, can be found at:https://doi.org/10.17605/OSF.IO/A98UF.
The interplay between cognition, functional and dual-task gait in persons with a vestibular disorder versus healthy controls
Authors: Pavlou Marousa , Costafreda Sergi G. , Galsworthy William , Korres George , Bamiou Doris-Eva 
Published: June 2023

AbstractClose links exist between vestibular function and cognition. Dual-task (DT) tests may have ecological validity to assess the impact of daily life cognitive-motor demands in people with vestibular dysfunction (PwVD), functional gait and falls risk. The present paper aimed at building predictive models for functional gait under DT conditions, while clarifying the impact of vestibular dysfunction, individual characteristics, varying task types and motor-cognitive demands. Case-controlled observational study with 39 PwVD and 62 healthy participants. The Functional Gait Assessment (FGA), with and without an additional motor, numeracy, or literacy task, was completed. Multiple linear regression was used to fit models to predict FGA under single and DT performance. Dual task cost (DTC, %) was calculated to assess DT interference on FGA performance using the equation: 100*(single task score–dual task score)/single-task score. Following Bonferroni corrections for multiple comparisons (corrected alpha level of 0.003), PwVD had poorer performance than controls for all FGA conditions (p < 0.001), motor (− 3.94%; p = 0.002) and numeracy (− 22.77%; p = 0.001) DTCs and spatial working memory (p = 0.002). The literacy DTC was marginally significant (− 19.39% p = 0.005). FGA single and DT motor, numeracy, and literacy models explained 76%, 76%, 66% and 67% of the variance respectively for PwVD. Sustained attention, visual memory and sex contributed to all models; short-term visual recognition memory, balance confidence, and migraine contributed to some models. Cognitive performance is impaired in PwVD. Motor, numeracy and literacy tasks impair functional gait performance. Cognitive assessment and FGA with a numeracy or literacy cognitive component should be included within assessment protocols and considered in the provision of targeted interventions for PwVD.
Characterization of a rhabdomyosarcoma reveals a critical role for SMG7 in cancer cell viability and tumor growth
Authors: Steiner Alexander J. , Zheng Yang , Tang Yi 
Published: June 2023

AbstractSoft-tissue sarcomas (STSs) are a rare and diverse group of mesenchymal cancers plagued with aggression, poor response to systemic therapy, and high rates of recurrence. Although STSs generally have low mutational burdens, the most commonly mutated genes are tumor suppressors, which frequently acquire mutations inducing nonsense-mediated mRNA decay (NMD). This suggests that STS cells may exploit NMD to suppress these anti-cancer genes. To examine the role that the NMD factor SMG7 plays in STS, we developed an inducible knockout mouse model in the Trp53−/− background. Here, we isolated a subcutaneous STS and identified it as a rhabdomyosarcoma (RMS). We report that knockout of SMG7 significantly inhibited NMD in our RMS cells, which led to the induction of NMD targets GADD45b and the tumor suppressor GAS5. The loss of NMD and upregulation of these anti-cancer genes were concomitant with the loss of RMS cell viability and inhibited tumor growth. Importantly, SMG7 was dispensable for homeostasis in our mouse embryonic fibroblasts and adult mice. Overall, our data show that the loss of SMG7 induces a strong anti-cancer effect both in vitro and in vivo. We present here the first evidence that disrupting SMG7 function may be tolerable and provide a therapeutic benefit for STS treatment.
Chicken skin based Milli Watt range biocompatible triboelectric nanogenerator for biomechanical energy harvesting
Authors: Khan Muhammad Umair , Mohammad Eman , Abbas Yawar , Rezeq Moh’d , Mohammad Baker 
Published: June 2023

AbstractThis work reports a high-performance, low-cost, biocompatible triboelectric nanogenerator (TENG) using chicken skin (CS). The device is suitable to power wearable devices, which is critical to adapt electronics in monitoring, predicting, and treating people. It also supports sustainability by providing a cost-effective way to reduce the poultry industry's waste. It has been shown here that CS-derived biowaste is an effective means of generating tribopositive material for TENGs. The CS contains amino acid functional groups based on (Glycine, Proline, and Hydroxyproline), which are essential to demonstrate the electron-donating ability of collagen. The skin was cut into 3 × 3 cm2and used as the raw material for fabricating the TENG device with a stacking sequence of Al/Kapton/spacing/CS/Al. The chicken skin-based TENG (CS-TENG) is characterized at different frequencies (4–14 HZ) using a damping system. The CS-TENG produces an open-circuit voltage of 123 V, short-circuit current of 20 µA and 0.2 mW/cm2of a power density at 20 MΩ. The biocompatible CS-TENG presents ultra-robust and stable endurance performance with more than 52,000 cycles. The CS-TENG is impressively capable of scavenging energy to light up to 55 commercial light-emitting diodes (LEDs), a calculator, and to measure the physiological motions of the human body. CS-TENG is a step toward sustainable, battery-less devices or augmented energy sources, especially when using traditional power sources, such as in wearable devices, remote locations, or mobile applications is not practical or cost-effective.
Development of a novel air–liquid interface airway tissue equivalent model for in vitro respiratory modeling studies
Authors: Leach Timothy , Gandhi Uma , Reeves Kimberly D. , Stumpf Kristina , Okuda Kenichi , Marini Frank C. , Walker Stephen J. , Boucher Richard , Chan Jeannie , Cox Laura A. , Atala Anthony , Murphy Sean V. 
Published: June 2023

AbstractThe human airways are complex structures with important interactions between cells, extracellular matrix (ECM) proteins and the biomechanical microenvironment. A robust, well-differentiated in vitro culture system that accurately models these interactions would provide a useful tool for studying normal and pathological airway biology. Here, we report the development and characterization of a physiologically relevant air–liquid interface (ALI) 3D airway ‘organ tissue equivalent’ (OTE) model with three novel features: native pulmonary fibroblasts, solubilized lung ECM, and hydrogel substrate with tunable stiffness and porosity. We demonstrate the versatility of the OTE model by evaluating the impact of these features on human bronchial epithelial (HBE) cell phenotype. Variations of this model were analyzed during 28 days of ALI culture by evaluating epithelial confluence, trans-epithelial electrical resistance, and epithelial phenotype via multispectral immuno-histochemistry and next-generation sequencing. Cultures that included both solubilized lung ECM and native pulmonary fibroblasts within the hydrogel substrate formed well-differentiated ALI cultures that maintained a barrier function and expressed mature epithelial markers relating to goblet, club, and ciliated cells. Modulation of hydrogel stiffness did not negatively impact HBE differentiation and could be a valuable variable to alter epithelial phenotype. This study highlights the feasibility and versatility of a 3D airway OTE model to model the multiple components of the human airway 3D microenvironment.
Cuticular profiling of insecticide resistant Aedes aegypti
Authors: Jacobs Ella , Chrissian Christine , Rankin-Turner Stephanie , Wear Maggie , Camacho Emma , Broderick Nichole A. , McMeniman Conor J. , Stark Ruth E. , Casadevall Arturo 
Published: June 2023

AbstractInsecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contributing mechanisms. Contact-based insecticides are absorbed through the insect cuticle, which is comprised mainly of chitin polysaccharides, cuticular proteins, hydrocarbons, and phenolic biopolymers sclerotin and melanin. Cuticle interface alterations can slow or prevent insecticide penetration in a phenomenon referred to as cuticular resistance. Cuticular resistance characterization of the yellow fever mosquito, Aedes aegypti, is lacking. In the current study, we utilized solid-state nuclear magnetic resonance spectroscopy, gas chromatography/mass spectrometry, and transmission electron microscopy to gain insights into the cuticle composition of congenic cytochrome P450 monooxygenase insecticide resistant and susceptible Ae. aegypti. No differences in cuticular hydrocarbon content or phenolic biopolymer deposition were found. In contrast, we observed cuticle thickness of insecticide resistant Ae. aegypti increased over time and exhibited higher polysaccharide abundance. Moreover, we found these local cuticular changes correlated with global metabolic differences in the whole mosquito, suggesting the existence of novel cuticular resistance mechanisms in this major disease vector.