bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Orai‐family channels in the activation and regulation of transcriptional activity
Authors: Nieto‐Felipe Joel , Macias‐Diaz Alvaro , Sanchez‐Collado Jose , Berna‐Erro Alejandro , Jardin Isaac , Salido Gines M. , Lopez Jose J. , Rosado Juan A. 
Published: March 2023

AbstractStore operated Ca2+ entry (SOCE) is a cornerstone for the maintenance of intracellular Ca2+ homeostasis and the regulation of a variety of cellular functions. SOCE is mediated by STIM and Orai proteins following the activation of inositol 1,4,5‐trisphosphate receptors. Then, a reduction of the endoplasmic reticulum intraluminal Ca2+ concentration is sensed by STIM proteins, which undergo a conformational change and activate plasma membrane Ca2+ channels comprised by Orai proteins. STIM1/Orai‐mediated Ca2+ signals are finely regulated and modulate the activity of different transcription factors, including certain isoforms of the nuclear factor of activated T‐cells, the cAMP‐response element binding protein, the nuclear factor κ‐light chain‐enhancer of activated B cells, c‐fos, and c‐myc. These transcription factors associate SOCE with a plethora of signaling events and cellular functions. Here we provide an overview of the current knowledge about the role of Orai channels in the regulation of transcription factors through Ca2+‐dependent signaling pathways.
Organization of cortical microtubules in differentiated cells
Authors: Zhou Ping , Yang Guiwen , Xie Wei 
Published: March 2023

AbstractThe microtubule cytoskeleton plays a critical role in a variety of cellular activities, and its structures and functions have been extensively studied. However, little is known about cell differentiation‐related microtubule remodeling, its regulatory mechanisms, and its physiological functions. Recent studies have shown that microtubule‐binding proteins as well as cell junctions, such as desmosomes and adherens junctions, are involved in the remodeling of microtubules in response to cell differentiation. In addition, the microtubule‐organizing activity and structural integrity of centrosomes undergo dramatic changes during cell differentiation to promote microtubule remodeling. Here we summarize recent advances revealing the dynamic changes in microtubule organization and functions during cell differentiation. We also highlight the molecular mechanisms underlying microtubule modeling in differentiated cells, focusing on the key roles played by microtubule‐binding proteins, cell junctions, and centrosomes.
Exploring the use of futures, values, systems, and strategic thinking among engineering education research collaborators
Authors: Dalal Medha , Carberry Adam , Archambault Leanna 
Published: March 2023

AbstractBackgroundMultiple reports call for new ways of thinking to address challenges facing engineering education in the coming decades. Adopting these approaches first requires identifying and understanding existing ways of thinking among those engaging in engineering education research.Purpose/HypothesisThis study is motivated by the recently published Framework for Applying Ways of Thinking in Engineering Education Research (FAWTEER). The purpose is to determine if and how the four ways of thinking proposed under FAWTEER—futures, values, systems, and strategic—are practiced by engineering education research collaborators.Design/MethodSeven engineer‐social scientist collaborator pairs across heterogeneous research projects were interviewed in dyads. Data were analyzed using an inductive coding approach that was informed by the framework and literature.ResultsEmergent findings provided evidence of collaborators' use of futures, values, systems, and strategic thinking, but the interpretations and relevance of a particular way of thinking varied depending on the project context. Proposal calls, organizational structures and policies, intentionality, and culture were identified as determinants that could foster or hinder the implementation of different ways of thinking.ConclusionsWays of thinking may be taken for granted or partly ignored in research practices despite their potential influence on innovation. This study highlights how futures, values, systems, and strategic thinking are enacted in engineering education research and suggests the need to further explore connections between ways of thinking and cultures that exist at the departmental, institutional, and disciplinary levels.
Acute toxicity of titanium dioxide microparticles in Artemia sp. nauplii instar I and II
Authors: de Paiva Pinheiro Sergimar Kennedy , Lima Ana Kamila Medeiros , Miguel Thaiz Batista Azevedo Rangel , Pireda Saulo , Fechine Pierre Basílio Almeida , Souza Filho Antonio Gomes , de Castro Miguel Emilio 
Published: March 2023

AbstractIn this study, the toxicity effects of titanium dioxide (MTiO2) microparticles on Artemia sp. nauplii instar I and II between 24 and 48 h was evaluated. The MTiO2 were characterized using different microscopy techniques. MTiO2 rutile was used in toxicity tests at concentration of 12.5, 25, 50, and 100 ppm. No toxicity was observed in Artemia sp. nauplii instar I at the time of 24 and 48 h. However, Artemia sp. nauplii instar II toxicity was observed within 48 h of exposure. MTiO2 at concentrations of 25, 50 and 100 ppm was lethal for Artemia sp. with a significant difference (p ≤ .05) in relation to the control artificial sea water with LC50 value at 50 ppm. Analysis of optical and scanning electron microscopy revealed tissue damage and morphological changes in Artemia sp. nauplii instar II. By using confocal laser scanning microscopy, cell damage was observed due to the toxicity of MTiO2 at a concentration of 20, 50, and 100 ppm. The high mortality rate is related to the filtration of MTiO2 by Artemia sp. nauplii instar II due to the complete development of the digestive tract.
Multidelay MR Arterial Spin Labeling Perfusion Map for the Prediction of Cerebral Hyperperfusion After Carotid Endarterectomy
Authors: Fan Xiaoyuan , Lai Zhichao , Lin Tianye , Li Kang , Hou Bo , You Hui , Wei Juan , Qu Jianxun , Liu Bao , Zuo Zhentao , Feng Feng 
Published: March 2023

BackgroundMultidelay arterial spin labeling (ASL) generates time‐resolved perfusion maps, which may provide sufficient and accurate hemodynamic information in carotid stenosis.PurposeTo use imaging markers derived from multidelay ASL magnetic resonance imaging (MRI) and to determine the optimal strategy for predicting cerebral hyperperfusion after carotid endarterectomy (CEA).Study TypeProspective observational cohort.SubjectsA total of 79 patients who underwent CEA for carotid stenosis.Field Strength/SequenceA 3.0 T/pseudo‐continuous ASL with three postlabeling delays of 1.0, 1.57, and 2.46 seconds using fast‐spin echo readout.AssessmentCerebral perfusion pressure, antegrade, and collateral flow were scored on a four‐grade ordinal scale based on preoperative multidelay ASL perfusion maps. Simultaneously, quantitative hemodynamic parameters including cerebral blood flow (CBF), arterial transit time (ATT), relative CBF (rCBF) and relative ATT (rATT; ipsilateral/contralateral values) were calculated. On the CBF ratio map obtained through dividing postoperative by preoperative CBF map, regions of interest were placed covering ipsilateral middle cerebral artery territory. Three neuroradiologists conducted this procedure. Cerebral hyperperfusion was defined as a CBF ratio >2.Statistical TestsWeighted κ values, independent sample t test, chi‐square test, Mann–Whitney U‐test, multivariable logistic regression analysis, receiver‐operating characteristic curve analysis, and Delong test. Significance level was P < 0.05.ResultsCerebral hyperperfusion was observed in 15 (19%) patients. Higher blood pressure (odd ratio [OR] = 1.08) and carotid near‐occlusion (NO; OR = 7.31) were clinical risk factors for postoperative hyperperfusion. Poor ASL perfusion score (OR = 37.33), decreased CBF (OR = 0.74), prolonged ATT (OR = 1.02), lower rCBF (OR = 0.91), and higher rATT (OR = 1.12) were independent imaging predictors of hyperperfusion. ASL perfusion score exhibited the highest specificity (95.3%), while CBF exhibited the highest sensitivity (93.3%) for the prediction of hyperperfusion. When combined with ASL perfusion score, CBF and ATT, the predictive ability was significantly higher than using blood pressure and NO alone (AUC: 0.98 vs. 0.78).Data ConclusionsMultidelay ASL can accurately predict cerebral hyperperfusion after CEA with high sensitivity and specificity.Evidence Level2Technical EfficacyStage 5
Feasibility of Quantitative MRI Using 3D‐QALAS for Discriminating Immunohistochemical Status in Invasive Ductal Carcinoma of the Breast
Authors: Amano Maki , Fujita Shohei , Takei Naoyuki , Sano Katsuhiro , Wada Akihiko , Sato Kanako , Kikuta Junko , Kuwatsuru Yoshiki , Tachibana Rina , Sekine Towa , Horimoto Yoshiya , Aoki Shigeki 
Published: March 2023

BackgroundTwo‐dimensional synthetic MRI of the breast has limited spatial coverage. Three‐dimensional (3D) synthetic MRI could provide volumetric quantitative parameters that may reflect the immunohistochemical (IHC) status in invasive ductal carcinoma (IDC) of the breast.PurposeTo evaluate the feasibility of 3D synthetic MRI using an interleaved Look–Locker acquisition sequence with a T2 preparation pulse (QALAS) for discriminating the IHC status, including hormone receptor (HR), human epidermal growth factor receptor 2 (HER 2), and Ki‐67 expression in IDC.Study TypeProspective observational study.PopulationA total of 33 females with IDC of the breast (mean, 52.3 years).Field Strength/SequenceA 3‐T, 3D‐QALAS gradient‐echo and fat‐suppressed T1‐weighted 3D fast spoiled gradient‐echo sequences.AssessmentTwo radiologists semiautomatically delineated 3D regions of interest (ROIs) of the whole tumors on the dynamic MRI that was registered to the synthetic T1‐weighted images acquired from 3D‐QALAS. The mean T1 and T2 were measured for each IDC.Statistical TestsIntraclass correlation coefficient for assessing interobserver agreement. Mann–Whitney U test to determine the relationship between the mean T1 or T2 and the IHC status. Multivariate logistic regression analysis followed by receiver operating characteristics (ROC) analysis for discriminating IHC status. A P value <0.05 was considered statistically significant.ResultsThe interobserver agreement was good to excellent. There was a significant difference in the mean T1 between HR‐positive and HR‐negative lesions, while the mean T2 value differed between HR‐positive and HR‐negative lesions, between the triple‐negative and HR‐positive or HER2‐positive lesions, and between the Ki‐67 level > 14% and ≤ 14%. Multivariate analysis showed that the mean T2 was higher in HR‐negative IDC than in HR‐positive IDC. ROC analysis revealed that the mean T2 was predictive for discriminating HR status, triple‐negative status, and Ki‐67 level.Data Conclusion3D synthetic MRI using QALAS may be useful for discriminating IHC status in IDC of the breast.Evidence Level1.Technical EfficacyStage 2.
Longitudinal antigenic and seroepidemiological analyses of parechovirus A1 in Yamagata, Japan
Authors: Mizuta Katsumi , Itagaki Tsutomu , Katsushima Fumio , Katsushima Yuriko , Sasaki Mika , Komabayashi Kenichi , Ikeda Yoko , Aoki Yoko , Matsuzaki Yoko 
Published: March 2023

AbstractTo investigate the antigenic changes in parechovirus 1 (PeVA1), seroepidemiological analyses were performed against the Harris strain (Harris), isolated in 1956, and PeVA1/Yamagata.JPN/2021‐4785, isolated in 2021, using immune sera and 207 and 237 human serum specimens collected in 2021 and 1976, respectively. Although rabbit immune sera showed the highest neutralization antibody (NT‐Ab) titers against the immunized viruses at 1:12 800–1:102 400, they were cross‐reactive at 1:400–1:800. All 62 Yamagata isolates obtained between 2001 and 2021 (Yamagata strains), belonging to phylogenetic lineage 1B, reacted more strongly (mostly 4–64 times) to antiserum against PeVA1/Yamagata.JPN/2021‐4785 than to antiserum against Harris, belonging to phylogenetic lineage 1 A. Human serum specimens obtained in 2021 showed higher NT‐Ab titers against PeVA1/Yamagata.JPN/2021‐4785, whereas those obtained in 1976 had similar NT‐Ab titers against both strains. These findings suggested that Yamagata strains and Harris were antigenically cross‐reactive, although there were differences. There are still high NT‐Abs titers present against Harris in 2021 in particular, indicating that PeVA1 has been in circulation with high immunity in the population. In conclusion, this study suggested that PeVA1 has been endemically perpetuated with only minor antigenic changes as well as with high immunity over several decades in the community.
NIR dye‐encoded nanotags for biosensing: Role of functional groups on sensitivity and performance in SERRS‐based LFA
Authors: Choi Namhyun , Adanalic Mujo , Dankov Asen , Grzeschik Roland , Hechler Michelle , Schlücker Sebastian 
Published: March 2023

AbstractSurface‐enhanced resonance Raman scattering (SERRS) is based on nanostructures supporting localized surface plasmon resonances as well as electronic resonances of the molecular adsorbate. Bright NIR‐SERRS nanotags require dyes with high Raman scattering cross‐sections upon NIR laser excitation in combination with functional groups for chemisorption. Here, we report on the role of isothiocyanate (ITC) and sulfonate (SO) groups in five cyanine dyes on sensitivity and performance of nanotags in SERRS‐based LFA. Dye concentration‐dependent SERRS and zeta potential measurements on bare gold nanostars (AuNS) revealed strong differences in their sensitivity and stability as a function of the number of ITC and SO groups. A positive correlation with the number of ITC groups due to stronger chemisorption and a negative correlation with the number of SO groups due to stronger desorption because of higher water solubility was observed. The application in SERRS‐based biosensing requires additional functionalization steps, for example, co‐adsorption of PEG with the NIR dye. The comparison of the Raman intensities before and after bioconjugation showed that the presence of at least one ITC group is necessary for minimizing Raman signal losses, irrespective of the additional presence of SO groups. In a SERRS‐based lateral flow assay (LFA) for sensing the pregnancy marker beta‐hCG, the two dyes NIR 4f and NIR 5e (both ITC = 2, SO = 0) showed the best performance. In contrast, we do not recommend to use IR‐783 (ITC = 0, SO = 2) for SERRS‐based biosensing. Overall, this study highlights the importance of surface‐seeking moieties such as the common ITC group for optimal performance in SERRS‐based biosensing platforms.
A nonmetal substrate of surface‐enhanced Raman spectroscopy for trace fentanyl detection
Authors: Lian Zheng , Lu Chunqing , Sun Qian , Cheng Jiaolong , Miao Cuiying , Chen Zhenqian 
Published: March 2023

AbstractRapid detection and identification of opioids are of great significance and an urgent need in drug regulation and forensic science. Fentanyl is an extremely potent synthetic drug of abuse that unfortunately cannot be accurately detected. Therefore, techniques combining fast, highly sensitive and portable for fentanyl detection need to be developed. Surface‐enhanced Raman spectroscopy (SERS) has emerged as a promising detection technique owing to its high sensitivity and selectivity. Here, we reported a nonmetal SERS substrate used for trace fentanyl detection. The lowest detectable concentration was 10−7 M, and the MoO2 substrate exhibited excellent thermal and chemical stability, which could even ensure high‐temperature heating, laser irradiation, and corrosion of strong acid or alkali.
Impact of different on‐farm management practices on bread wheat quality: a case study in the Yaqui Valley
Authors: Tabbita Facundo , Ortiz‐Monasterio Iván , Piñera‐Chavez Francisco J. , Ibba María Itria , Guzmán Carlos 
Published: March 2023

AbstractBACKGROUNDContinuous development of new wheat varieties is necessary to satisfy the demands of farmers, industry, and consumers. The evaluation of candidate genotypes for commercial release under different on‐farm conditions is a strategy that has been strongly recommended to assess the performance and stability of new cultivars in heterogeneous environments and under different farming systems. The main objectives of this study were to evaluate the grain yield and quality performance of ten different genotypes across six contrasting farmers' field conditions with different irrigation and nitrogen fertilization levels, and to develop suggestions to aid breeding programs and farmers to use resources more efficiently. Genotype and genotype by environment (GGE) interaction biplot analyses were used to identify the genotypes with the strongest performance and greatest stability in the Yaqui Valley.RESULTSAnalyses showed that some traits were mainly explained by the genotype effect, others by the field management conditions, and the rest by combined effects. The most representative and diverse field conditions in the Yaqui Valley were also identified, a useful strategy when breeders have limited resources. The independent effects of irrigation and nitrogen levels and their interaction were analyzed for each trait. The results showed that full irrigation was not always necessary to maximize grain yield in the Yaqui Valley. Other suggestions for more efficient use of resources are proposed.CONCLUSIONSThe combination of on‐farm trials with GGE interaction analyses is an effective strategy to include in breeding programs to improve processes and resources. Identifying the most outstanding and stable genotypes under real on‐farm systems is key to the development of novel cultivars adapted to different management and environmental conditions. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Neoadjuvant chemotherapy for pancreatic cancer: Quality over quantity
Authors: Thalji Sam Z. , Aldakkak Mohammed , Christians Kathleen K. , Clarke Callisia N. , George Ben , Kamgar Mandana , Erickson Beth A. , Hall William A. , Chisholm Phillip , Kulkarni Naveen , Doucette Saryn , Evans Douglas B. , Tsai Susan 
Published: March 2023

AbstractBackground and ObjectivesThe ideal duration of neoadjuvant chemotherapy (NACT) in patients with localized pancreatic adenocarcinoma (PDAC) treated with curative intent is unclear. We sought to determine the prognostic significance of both duration of NACT and Carbohydrate Antigen 19‐9 (CA19‐9) normalization to NACT.MethodsWe examined patients with resectable and borderline resectable PDAC treated with NACT and chemoradiation. Patients were compared by NACT duration (2 vs. 4 months) and by CA19‐9 normalization after NACT.ResultsAmong 171 patients, 83 (49%) received 2 months of NACT, and 88 (51%) received 4 months. After NACT completion, 115 (67%) patients had persistently elevated CA19‐9, and 56 (33%) had normalized. Of the 125 patients who had successful surgery, 73 (58%) had normalized CA19‐9 postoperatively. Duration of NACT was not associated with overall survival (OS) while CA19‐9 normalization after NACT (regardless of duration) was associated with improved OS (hazard ratio [HR] 0.56, 95% confidence interval [CI] 0.35–0.89, p = 0.02). Adjuvant chemotherapy was associated with improved OS among patients without CA19‐9 normalization after NACT (HR 0.42, CI 0.20–0.86, p = 0.02) but not among those that normalized, independent of duration.ConclusionsCA19‐9 normalization after NACT is a clinically significant endpoint of treatment; patients without CA19‐9 normalization may benefit from additional therapy.
In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to Treat Pressure Ulcers
Authors: Shan Jingyang , Li Xiuping , Huang Zhengzheng , Kong Bin , Wang Huan , Ren Lijie 
Published: March 2023

AbstractHow to avoid the microenvironment limitations in the therapeutic process of pressure ulcers is still challenging. The development of a functional gel can kill bacteria and scavenge reactive oxygen species (ROS), which is urgently required in the therapeutic process of pressure ulcers. Herein, an in situ sprayed gel is developed with silver nanoparticles (AgNPs) and polydopamine (PDA) NPs (APG) to obviate microenvironment restrictions in treating pressure ulcers. The gel is constructed by spraying sodium alginate solution and CaCl2 solution. AgNPs serve as an antibacterial agent in the formed gel, which can effectively cause bacterial inactivation and show more than 5 log (>99.999%) bacterial killing efficiency against methicillin‐resistant S. aureus (MRSA), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) in vitro. Meanwhile, PDA NPs serve as the antioxidative agent in the formed gel, which can facilitate the elimination of ROS to address the high ROS problem in wound microenvironment. Based on these features, it is demonstrated through cell and animal experiments that the AgNPs and PDA NPs incorporated gel can realize the effective treatment of MRSA‐infected and hydrogen peroxide (H2O2)‐sensitized pressure ulcers. It is believed that the designed system by a simple spray‐coating approach can provide a new therapeutic strategy in biomedical areas.
Mitigation of top‐ and bottom‐of‐the‐line CO2 corrosion in the presence of acetic acid (II): Inhibition using azole derivatives
Authors: Ajayi Fredric O. , Lyon Stuart 
Published: March 2023

AbstractCarbon steel flowlines transporting hydrocarbon fluids are susceptible to internal corrosion in the aqueous phase at the base (6 o'clock position) and in aqueous condensed droplets at the top (12 o'clock position). Respectively, these issues are known as “bottom‐of‐the‐line” corrosion and “top‐of‐the‐line” corrosion and inhibitors that are used to control internal pipeline corrosion need to be effective at both locations. Here, we explore whether 2‐mercaptobenzimidazole (2‐MBI), 2‐phenyl‐2‐imidazoline (2‐PI), 2‐amino‐5‐ethyl‐1,3,4‐thiodiazole (2‐AETD), are able to control CO2 corrosion simultaneously at both the top‐ and the bottom‐of‐the‐line in the presence and absence of acetic acid, a common minor constituent of produced hydrocarbon fluids. The performance of the species varied between highly effective (2‐MBI), moderately effective (2‐AETD) to ineffective (2‐PI). Inhibition was effective at both bottom‐ and top‐of‐the‐line, and with acetic acid present. Given that the vapor pressure of these species is negligible, it is suggested that they are carried from the bulk phase to the top‐of‐the‐line dissolved in aerosol droplets rather than in the vapor phase.
Microstructure, mechanical and corrosion behaviours in friction stir welding of dissimilar magnesium alloys
Authors: Singh Umesh K. , Dubey Avanish K. 
Published: March 2023

AbstractUsing magnesium alloys reduce the weight of a vehicle considerably, but the available fusion welding methods impose restrictions on the fabrication of magnesium alloy components. Friction stir welding is an efficient method to weld magnesium alloys, but due to extreme plasticization and stirring of materials, the microstructures are transformed. Similar to other alloys, friction stir welded magnesium alloys are also susceptible to corrosion. In the present work, dissimilar AZ31 and AZ91 Mg alloys were friction stir welded at varying welding conditions. The variable presence of small cracks with a high amount of oxide, and microstructural and textural differences result in varying joint strength, that is, joint efficiency, which has a maximum value of approximately 90% of a stronger base material. The maximum corrosion potential obtained in welded zones is approximately equal to a base material that conveys similar electrochemical behaviour. The minimum corrosion rate of 0.22 mm/year is observed in the sample welded at 700 rpm tool rotational speed, 30 mm/min welding speed and 18 mm shoulder diameter.