bluuebunny/crossref_metadata_2025_split huggingface dataset:
Five-year follow-up of a phase I trial of donor-derived modified immune cell infusion in kidney transplantation
Authors: Schaier Matthias , Morath Christian , Wang Lei , Kleist Christian , Opelz Gerhard , Tran Thuong Hien , Scherer Sabine , Pham Lien , Ekpoom Naruemol , Süsal Caner , Ponath Gerald , Kälble Florian , Speer Claudius , Benning Louise , Nusshag Christian , Mahler Christoph F. , Pego da Silva Luiza , Sommerer Claudia , Hückelhoven-Krauss Angela , Czock David , Mehrabi Arianeb , Schwab Constantin , Waldherr Rüdiger , Schnitzler Paul , Merle Uta , Schwenger Vedat , Krautter Markus , Kemmner Stephan , Fischereder Michael , Stangl Manfred , Hauser Ingeborg A. , Kälsch Anna-Isabelle , Krämer Bernhard K. , Böhmig Georg A. , Müller-Tidow Carsten , Reiser Jochen , Zeier Martin , Schmitt Michael , Terness Peter , Schmitt Anita , Daniel Volker 
Published: July 2023

BackgroundThe administration of modified immune cells (MIC) before kidney transplantation led to specific immunosuppression against the allogeneic donor and a significant increase in regulatory B lymphocytes. We wondered how this approach affected the continued clinical course of these patients.MethodsTen patients from a phase I clinical trial who had received MIC infusions prior to kidney transplantation were retrospectively compared to 15 matched standard-risk recipients. Follow-up was until year five after surgery.ResultsThe 10 MIC patients had an excellent clinical course with stable kidney graft function, no donor-specific human leukocyte antigen antibodies (DSA) or acute rejections, and no opportunistic infections. In comparison, a retrospectively matched control group receiving standard immunosuppressive therapy had a higher frequency of DSA (log rank P = 0.046) and more opportunistic infections (log rank P = 0.033). Importantly, MIC patients, and in particular the four patients who had received the highest cell number 7 days before surgery and received low immunosuppression during follow-up, continued to show a lack of anti-donor T lymphocyte reactivity in vitro and high CD19+CD24hiCD38hi transitional and CD19+CD24hiCD27+ memory B lymphocytes until year five after surgery.ConclusionsMIC infusions together with reduced conventional immunosuppression were associated with good graft function during five years of follow-up, no de novo DSA development and no opportunistic infections. In the future, MIC infusions might contribute to graft protection while reducing the side effects of immunosuppressive therapy. However, this approach needs further validation in direct comparison with prospective controls.Trial registrationhttps://clinicaltrials.gov/, identifier NCT02560220 (for the TOL-1 Study). EudraCT Number: 2014-002086-30.
Interrogation of human microglial phagocytosis by CRISPR genome editing
Authors: Chang Jason Cheng-Yu , Wang Cheng-You , Lin Steven 
Published: July 2023

BackgroundMicroglia are an integral part of central nervous system, but our understanding of microglial biology is limited due to the challenges in obtaining and culturing primary human microglia. HMC3 is an important cell line for studying human microglia because it is readily accessible and straightforward to maintain in standard laboratories. Although HMC3 is widely used for microglial research, a robust genetic method has not been described. Here, we report a CRISPR genome editing platform, by the electroporation of Cas9 ribonucleoproteins (Cas9 RNP) and synthetic DNA repair templates, to enable rapid and precise genetic modifications of HMC3. For proof-of-concept demonstrations, we targeted the genes implicated in the regulation of amyloid beta (Aβ) and glioblastoma phagocytosis in microglia. We showed that CRISPR genome editing could enhance the phagocytic activities of HMC3.MethodsWe performed CRISPR gene knockout (KO) in HMC3 by the electroporation of pre-assembled Cas9 RNP. Co-introduction of DNA repair templates allowed site-specific knock-in (KI) of an epitope tag, a synthetic promoter and a fluorescent reporter gene. The editing efficiencies were determined genotypically by DNA sequencing and phenotypically by immunofluorescent staining and flow cytometry. The gene-edited HMC3 cells were examined in vitro by fluorescent Aβ and glioblastoma phagocytosis assays.ResultsOur platform enabled robust single (&gt;90%) and double (&gt;70%) KO without detectable off-target editing by high throughput DNA sequencing. We also inserted a synthetic SFFV promoter to efficiently upregulate the expression of endogenous CD14 and TREM2 genes associated with microglial phagocytosis. The CRISPR-edited HMC3 showed stable phenotypes and enhanced phagocytosis of fluorescence-labeled Aβ1-42 peptides. Confocal microscopy further confirmed the localization of Aβ1-42 aggregates in the acidified lysosomes. HMC3 mutants also changed the phagocytic characteristic toward apoptotic glioblastoma cells.ConclusionCRISPR genome editing by Cas9 RNP electroporation is a robust approach to genetically modify HMC3 for functional studies such as the interrogation of Aβ and tumor phagocytosis, and is readily adoptable to investigate other aspects of microglial biology.
Eastern High Arctic–Baffin Bay beluga whale (Delphinapterus leucas) estuary abundance and use from space
Authors: Watt Cortney A. , Carlyle Cody G. , Hornby Claire A. , Sherbo Bryanna A. H. 
Published: July 2023

IntroductionThe Eastern High Arctic–Baffin Bay (EHA-BB) beluga whale (Delphinapterus leucas) population spends summer in estuaries around Somerset Island, Nunavut, Canada. A single abundance estimate from 1996 suggests an abundance &gt;21,000 beluga whales; however, more information on abundance and distribution is needed to ensure effective management of this population, especially in estuaries where previous surveys provided minimal coverage. To assess the feasibility of using Very High Resolution (VHR) satellite imagery to obtain estuary abundance estimates for this beluga population, we evaluated a citizen science crowd counting initiative that was designed to monitor remote beluga whale populations and their estuary use.MethodsIn July and August 2020 the WorldView 2 and 3, and GeoEye 1 satellites were tasked to collect VHR imagery (30–41 cm) of estuaries previously known to be used by Eastern High Arctic–Baffin Bay beluga whales. The objectives were to obtain an estuary abundance estimate for this population from satellite imagery, and to evaluate the effectiveness of having imagery annotated using a crowd-source platform. Almost 3,800 km2of ocean imagery was analyzed using Maxar’s Geospatial Human Imagery Verification Effort (GeoHIVE) Crowdsourcing platform. Expert readers then manually compared counts to those performed by crowd-counters to determine variance in observer counts.Results and DiscussionThe estuary abundance estimate from 11 core estuaries was 12,128 (CV 36.76%, 95% confidence interval 6,036–24,368) beluga whales. This represents an estuary abundance estimate only, as the greater Peel Sound and Prince Regent Inlet areas were not photographed. The estuaries with the largest abundance of beluga whales were Creswell Bay, Maxwell Bay, and Prince Whales Island, with over 2,000 crowd-counted whales in each estuary. Although VHR imagery has potential to assist with surveying and monitoring marine mammals, for larger estuaries it was not always possible to photograph the entire area in a single day, and cloud cover was an issue for sections of most images. This work will assist with planning large-scale aerial surveys for monitoring beluga whale populations, identifying high-use areas and important beluga habitat, and highlights the utility of using VHR imagery to enhance our understanding of estuary abundance and distribution of Arctic whales.
Insights into nutrition, flavor and edible quality changes of golden pomfret (Trachinotus ovatus) fillets prepared by different cooking methods
Authors: Xiong Tian , Mei Xin , Wu Yanyan , Wang Lan , Shi Jianbin , Sui Yong , Cai Sha , Cai Fang , Chen Xueling , Fan Chuanhui 
Published: July 2023

IntroductionIn recent years, Asia has seen an increase in demand for golden pomfret (Trachinotus ovatus). Especially in instant (ready-to-eat) and prepared (ready-to-cock) food processing industry. Thermal processing is a vital part of food processing. However, no comprehensive analysis has been reported on its flavor, nutrition and edible quality changes during the key thermal processing.MethodsAccordingly, in this study, we evaluated the effects of different cooking methods (steaming, frying, microwaving and baking) on the color, texture, cooking loss, nutrition composition, volatile flavor substances and other indicators of golden pomfret filets.Results and DiscussionThe results showed that the steamed samples (SS) had the lowest cooking loss and fat content, the highest moisture content, complete appearance and the lowest levels of hardness and chewiness. Fried samples (FS) had a notable difference in fatty acid composition. The content of unsaturated fatty acids (UFAs) increased significantly, while the relative content of eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) decreased from 7.88 to 1.42%, lower than other groups. The essential amino acid index (EAAI) of microwaved samples (MS) was 94.89, which was higher than other groups. Baked samples (BS) had the highest relative content of umami amino acids (UAAs) and sweet amino acids (SAAs), which was 8.08 mg/100 mg and 5.19 mg/100 mg, respectively. Hexanal and nonanal were detected in control samples (CK), SS, FS, MS and BS. While pyrazine compounds were detected only in FS and BS. Steaming and microwaving treatment of golden pomfret resulted in better nutritional preservation, which was more conducive to human health. Frying and baking treatment of golden pomfret had better taste and flavor and higher sensory scores. The nutrition, flavor and edible quality of golden pomfret under different cooking methods were related and interactive. Cooking loss and fat content can be used as simple evaluation indicators to compare the overall quality of different cooking methods. This study provides a reference for the thermal processing technology and industrial production of golden pomfret.
Addressing child and adolescent obesity management in Ireland: identifying facilitators and barriers in clinical practice
Authors: Ferdous Farzana , Arthurs Niamh , Tully Louise , O’Brien Sarah , Smith Susan M. , Walsh Aisling , O’Gorman Clodagh S. , O’Malley Grace 
Published: July 2023

BackgroundIreland’s Model of Care for the Management of Overweight and Obesity outlines a plan for treating adolescent and child obesity (CO). However, engagement with key stakeholders is required to support its implementation and improve health services.AimThis study aims to map the perceived barriers and facilitators related to CO management across healthcare settings, professional disciplines, and regions in the Republic of Ireland (ROI).Materials and methodsAn online cross-sectional survey of registered healthcare professionals (HPs), designed to adhere to the Consolidated Framework for Implementation Research (CFIR), was co-developed by a project team consisting of researchers, healthcare professionals, and patient advocates. The survey was pilot tested with project stakeholders and distributed online to professional groups and via a social media campaign, between September 2021 and May 2022, using “SurveyMonkey.” Data were summarised using descriptive statistics and thematic analyses. Themes were mapped to the CFIR framework to identify the type of implementation gaps that exist for treating obesity within the current health and social care system.ResultsA total of 184 HPs completed the survey including nurses (18%), physicians (14%), health and social care professionals (60%), and other HPs (8%). The majority were female (91%), among which 54% reported conducting growth monitoring with a third (32.6%) giving a diagnosis of paediatric/adolescent obesity as part of their clinical practice. Nearly half (49%) of the HPs reported having the resources needed for clinical assessment. However, 31.5% of the HPs reported having enough “time,” and almost 10% of the HPs reported having no/limited access to suitable anthropometric measurement tools. Most HPs did not conduct obesity-related clinical assessments beyond growth assessment, and 61% reported having no paediatric obesity training. CFIR mapping identified several facilitators and barriers including time for clinical encounters, suitable materials and equipment, adequate training, perceived professional competency and self-efficacy, human equality and child-centredness, relative priorities, local attitudes, referral protocols, and long waiting times.ConclusionsThe findings provide actionable information to guide the implementation of the Model of Care for the Management of Overweight and Obesity in Ireland. Survey findings will now inform a qualitative study to explore implementation barriers and facilitators and prioritise actions to improve child and adolescent obesity management.
Safety and efficacy of oil palm phenolic supplementation in improving lipid profile among hyperlipidemic adults: a phase 2, randomized, double-blind, placebo-controlled clinical trial
Authors: Muhammad Ismail Tadj Nur Balqis , Ibrahim Nurul `Izzah , Tg Abu Bakar Sidik Tg Mohd Ikhwan , Zulfarina Mohamed S. , Haji Mohd Saad Qodriyah , Leow Soon-Sen , Fairus Syed , Naina Mohamed Isa 
Published: July 2023

Introduction:Oil palm phenolic (OPP) is an antioxidant aqueous palm oil by-product and contains a high amount of phenolics. OPP has been proven to have many therapeutical benefits, and one of them is as an antihyperlipidemic agent. The previous phase 1 clinical trial proved OPP was safe to be orally consumed by healthy volunteers and yielded a good lipid profile. Thus, this phase 2 clinical trial was conducted to determine the effectiveness of OPP in improving the lipid profile among hyperlipidemic subjects.Methods:A parallel, placebo-controlled, randomized, double-blinded clinical trial was conducted for 2 months on 50 hyperlipidemic subjects aged 20–50 years old. The subjects were randomly distributed to two treatment arms with 25 participants each: control/placebo (11 males and 14 females) and 250 mg of OPP (10 males and 15 females). The subjects were required to consume one capsule per day for 60 days. Fasting blood sampling for routine blood profile (hematology, liver function, renal function, and lipid) analysis and a medical examination were conducted at baseline, day 30, and day 60.t-test analysis was used to compare the difference between two test groups.Results:The baseline lipid profile between control group (TC, 5.78 ± 0.52 mmol/L; LDL, 3.88 ± 0.51 mmol/L; HDL, 1.30 ± 0.25; TG, 1.30 ± 0.82), and 250 mg OPP (TC, 5.76 ± 0.54 mmol/L; LDL, 3.82 ± 0.59 mmol/L; HDL, 1.37 ± 0.34; TG, 1.25 ± 0.54) is insignificant. No serious adverse events (SAEs) were reported. No abnormality in fasting blood parameters in all groups was found. Compared to the control group among male participants, the 250 mg OPP group showed an improved serum triglyceride level. There were no statistically significant changes in all blood parameters from day 1 to day 60 with the exception of triglyceride level.Conclusion:The absence of SAEs reported and no abnormal findings in biochemistry and hematology results suggested that the 250 mg OPP was safe to be taken by hyperlipidemic patients with a high probability of reducing triglyceride level in hyperlipidemic male patients The outcomes from this phase II trial suggest that by incorporating OPP supplements into the diet may be a promising strategy for individuals with hyperlipidemia to improve their lipid profiles and reduce cardiovascular risk. However, more research is needed to fully understand the mechanisms of action and establish the long-term efficacy and safety of OPP supplementation in larger scale.Limitation:Small samples size hence lack of diversity (25 subjects per groups) and early sharing of treatment-response results.Clinical Trial Registration:clinicaltrials.gov, identifier NCT04573218.
Lived experience perspectives guiding improvements to the Systematic Tailored Assessment for Responding to Suicidality protocol
Authors: Hawgood Jacinta , Betterridge Carmen , Kõlves Kairi , Edwards Bronwen , Spence Susan H. , Arensman Ella , De Leo Diego , Ownsworth Tamara 
Published: July 2023

Background and aimsSuicide risk assessment protocols have traditionally been developed by clinical or research experts in suicidology, with little formal involvement of those with a lived experience of suicide. This study broadly aimed to seek lived experience perspectives of the Systematic Tailored Assessment for Responding to Suicidality (STARS) protocol A further aim was to elicit lived experience suggestions for wording and language used in the existing items within sections of the STARS protocol (STARS-p).MethodParticipants were 33 adults (Female = 64%) with a lived experience of suicide, who attended a virtual research workshop at the National Lived Experience of Suicide Summit (2021). After being educated about STARS-p, participants provided their overall perceptions of STARS-p as well as suggestions for rewording and language use across the sections of STARS-p. Their responses were gathered using a virtual online platform for live electronic data collection. A three-phase process of qualitative content analysis was used, engaging both inductive and deductive approaches to explore study aims one and two, respectively. The Consolidated Criteria for Reporting Qualitative Research was followed to enhance quality of reporting.ResultsQualitative content analysis of participants’ views of the STARS-p reflected three main categories, namely, STARS philosophy; What STARS aspires to; and Continuity of care and meeting needs. Responses characterized participants’ perceptions of the core purpose of STARS-p and ways for refining or adapting it to suit diverse needs and settings. Based on deductive content analysis, suggested modifications to wording of items and additional items to extend sections were identified.ConclusionThe study yielded novel perspectives from those with a lived experience of suicide, which will inform improvements to the next edition of STARS-p. The STARS training (required for licensed use of the protocol) will be updated accordingly, in line with these results.
Sociodemographic and clinical features of men and women with eating disorders: a diagnosis-matched, retrospective comparison among inpatients
Authors: Traut Philipp , Halbeisen Georg , Braks Karsten , Huber Thomas J. , Paslakis Georgios 
Published: July 2023

IntroductionEating disorders (EDs) are among the most severe mental disorders in women and men, often associated with high symptom burden and significant limitations in daily functioning, frequent comorbidities, chronic course of illness, and even high mortality rates. At the same time, differences between men and women with EDs remain poorly explored.MethodsIn this study, we compared 104 men to 104 diagnosis-matched women with EDs regarding sociodemographic and clinical features. Using latent class mixture modelling, we identified four distinct patient subgroups based on their sociodemographic features.ResultsMen with EDs had significantly higher odds than women to belong to a “single-childfree-working” class. Moreover, while there were few overall differences in ED-related symptoms and general psychopathology between men and women, single-childfree-working men with EDs presented with higher general psychopathology symptoms than men in the other classes.DiscussionWe discuss how considering sex and gender along with further sociodemographic differences in EDs may help to improve ED diagnosis and treatment.
Understanding older adults’ smartphone addiction in the digital age: empirical evidence from China
Authors: Xu Yujing , Zeng Kai , Dong Lucong , Zheng Xiaofen , Si Yuxiu 
Published: July 2023

BackgroundDespite the fact that an increasing number of older adults are addicted to smartphones, the existing addiction literature still focuses primarily on adolescents. To address this issue, this study draws from the perspectives of subjective cognitive decline and family relationship conflict to examine older adults’ smartphone addiction based on their key characteristics.MethodsThis study investigates the effects of subjective cognitive decline and family relationship conflict on older adults’ smartphone addiction through a survey of 371 subjects in China.ResultsThe results show that subjective cognitive decline and family relationship conflict affect older adults’ smartphone addiction through a sense of alienation. In addition, older adults’ perceived power moderates the relationship between alienation and smartphone addiction.DiscussionThis study offers new perspectives on the study of smartphone addiction from the perspective of older adults, and sheds light on how to improve the older adults’ quality of life in their later years.
Factors associated with food insecurity among the chronically ill population during the COVID-19 pandemic in the United States
Authors: Dean Caress A. , Liu Echu , Enard Kimberly R. , Qian Zhengmin , Elder Keith T. 
Published: July 2023

IntroductionLittle is known about food insecurity among Americans with chronic diseases, one of the vulnerable groups in health care. Factors influencing food insecurity among this population group are especially poorly understood.MethodsUsing data from the COVID Impact Survey, this cross-sectional study sought to examine food insecurity among adults with chronic diseases in the United States and to identify factors associated with their risks for food insecurity during the COVID-19 pandemic.ResultsNearly 28% of the national and 32% of the regional samples from the COVID Impact Survey were at risk for food insecurity. The logistic regressions show that chronically ill US adults with one of the following characteristics have higher odds of being at risk for food insecurity: younger than 60 years, having financial stress, unemployed, having received food from a food pantry, without health insurance, having a household income lower than $100,000, and without a college degree.DiscussionTargeted policies and programs are warranted to address underlying determinants of food insecurity that adults with chronic illnesses experience.
Sports-related injuries sustained by officers of the State Fire Service on duty – nationwide 7-year follow-up
Authors: Dudziński Łukasz , Panczyk Mariusz , Kubiak Tomasz , Milczarczyk Tomasz 
Published: July 2023

AimThe accident rate in the State Fire Service from 2015 to 2021 related to sports activities was analyzed in relation to the regions of the country per year.Materials and methodsThe study included analysis of data from the SFS Headquarters – Department for Occupational Health and Safety and Preventive Health. Data collected from across the country in the form of an annual analysis of the accident. The reports included such information as: the number of accidents, the cause and circumstances of accident (injury), with a breakdown listing individual and group accidents.ResultsDuring the observation period, about 30,000 officers were on duty in the SFS, of which about 20% were on daily (8-h) duty, and 80% were on shift (24-h) duty. Between 2015 and 2021, there were N = 11,332 (Mean: 1617.4; SD: 284.1) accidents in SFS. Total accident covers individual and mass accidents. The number of sports injuries was N = 4,254 (Mean: 532.2; SD: 137.9).ConclusionThere is a need for comprehensive approach to physical training in the firefighter population. Physical activity should be continuous and systematic strengthening of the whole body. Sports activities should begin with performing thorough warm-ups. It is necessary to maintain facilities, premises, equipment and technical devices in a condition that sets the ground for doing sports safely and in a hygienic manner. Most of the sports injuries sustained by firefighters are related to team sports.