bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor necrosis factor receptor-associated protein 1 promotes aerobic glycolysis and cisplatin resistance by regulating Wnt/β-catenin signal pathway in lung cancer
Authors: Li Ruijie , Li Mengguo , Lv Juan , Sun Qian 
Published: March 2023

Abstract
Objective: To investigate the effect of tumor necrosis factor receptor-associated protein 1 (TRAP1) on aerobic glycolysis in cisplatin-resistant lung cancer and its possible mechanism.
Methods: The expression of TRAP1 in cisplatin resistant lung cancer tissues and A549/CDDP cells were detected. The expression of TRAP1 in A549/CDDP cells were silenced by transfection siRNA. The changes of lactate content, glucose consumption and the expression of lactate dehydrogenase A (LDHA), hexokinase 2 (HK2) and pyruvate kinase M2 (PKM2) and sensitivity to cisplatin were detected. The change of Wnt/β-catenin signaling pathway was detected, and the Wnt/β-catenin activator BML-284 was used to conduct reverse expression.
Results TRAP1 expression was increased in cisplatin-resistant tissues and A549/CDDP cells compared with cisplatin-sensitive tissues and A549, respectively (P<0.05); After silencing TRAP1, the lactic content, glucose consumption, LDHA, HK2, PKM2 expression, IC50 concentration of cisplatin were significantly increased (P<0.05). Compared with A549, Wnt/β-catenin pathway was activated in A549/CDDP, and inhibited after TRAP1 silencing. BML-284 can reverse the effects of silencing TRAP1 on aerobic glycolysis and cisplatin sensitivity of A549/CDDP.
Conclusion TRAP1 is involved in the cisplatin resistance in lung cancer, and its mechanism is related to the regulation of aerobic glycolysis by regulating Wnt/β-catenin pathway.
Therapeutic angiogenesis with intramuscular SeV-hFGF2/dF for the treatment of peripheral arterial disease: a phase I clinical trial
Authors: Lian Wenzhuo , Li Fengshi , Rong Zhihua , Wang Peng , Di Xiao , Song Xitao , Ni Leng , Liu Changwei 
Published: March 2023

Abstract
This paper reports a phase I dose-escalation clinical trial for the treatment of peripheral arterial disease (PAD) with BF30, a recombinant Sendai virus vector carrying the human native fibroblast growth factor 2 (hFGF2) gene. Our goal was to evaluate the safety and tolerability of BF30 at varying doses while assessing its preliminary therapeutic effect. Twelve male patients with PAD unsuitable for revascularization procedures that met the enrollment criteria were recruited. Each patient received a BF30 injection on one side of the ischemic lower limb. No deaths occurred prior to the 6-month follow-up. No severe adverse events or Grade 3/4 adverse events related to the BF30 injections were noted. Furthermore, the viral genome level was below the quantization limit after 6 months, and hemagglutination activity was undetectable in seven of the 12 cases. Abnormal changes in hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and proinflammatory factor serum levels were not observed. None of the cases exhibited a positive anti-drug antibody signal. In addition, a positive therapeutic effect was demonstrated by the reduction in rest pain and Rutherford classification and improvement of the ankle-brachial index. While a higher dose triggered a stronger immune response, it was more effective in improving the Rutherford classification and quality of life.
Model of non-stationary heat transfer in a supercritical fluid
Authors: Melkikh A. V. , Rutin S. B. , Skripov P. V. 
Published: March 2023

Abstract
This paper continues the process of reconciling results obtained when investigating heat transfer in the supercritical liquid-vapour region inherent in stationary and fast processes. A relatively simple model of non-stationary heat transfer at the microscopic level in a non-idealised system is constructed. The model provides a possible explanation for the increase in the thermal resistance of a supercritical fluid (drop in heat conduction) at a not too great distance from the critical isobar on a scale of small characteristic times and sizes. The model is based on an explicit account of a significant decrease in thermal diffusivity when approaching the critical temperature of the substance. The simulation results are compared with experimental data on the rapid (lasting in units-tens of milliseconds) transfer of a compressed liquid to the supercritical temperature region along a supercritical isobar.
Prevalence, trend and associated factors of malaria in the Shai-Osudoku District Hospital
Authors: Tetteh Jessica Ashiakie , Djissem Patrick Elorm , Manyeh Alfred Kwesi 
Published: March 2023

Abstract
Background
Even though malaria is easily preventable and treatable, it continues to have a devastating impact on people’s health and livelihoods around the world. Sub-Saharan Africa carries a disproportionately high share of the global malaria burden. This study seeks to assess the prevalence, trend and factors associated with malaria in the Shai-Osudoku District Hospital.
Methods
A cross-sectional study was conducted to determine the prevalence, trend, and factors associated with malaria in the Shai-Osudoku District Hospital. A 10-month secondary data was extracted from February to November 2020 in the Shai-Osudoku District Hospital, Ghana. The extracted data were entered into Epi Data version 6 and analyzed using STATA version 16. Descriptive analysis was performed to determine the prevalence, trend and socio-demographic characteristics of study participants. Simple logistic regression at a 95% confidence level was performed to investigate socio-demographic factors associated with malaria infection. Tables and charts with summary statistics were used to present the results.
Results
Secondary data from 3896 individuals were included in the study. The participants with The age of the participants range from 0.8 to 101 years with a mean age of 32.5. The estimated prevalence of malaria during the study period is 20.9%. Majority (79.1%) of the participants who presented signs and symptoms of malaria were negative after testing. The prevalence of malaria cases increased progressively from 6.7–55.4% across the ten months. The simple logistic regression at a 95% confidence level revealed that age group, sex, residential status, religion, occupation and marital status were statistically significantly associated with malaria. The shows that persons who tested positive for malaria were mostly treated with Artemether lumefantrine (46.1%), some malaria positive cases were given Artesunate injection (11.6%), Dihydroartenisinin Piperaquine (16.2%) and Oral artemether-lumefantrine (6.5%). Surprisingly 19.6% of the malaria-positive cases were not given any form of malaria medication.
Conclusion
Factors found to influence malaria infection in the Shai-Osudoku District Hospital include participant’s age, sex, residential status, religious affiliation occupation and marital status. The findings of this study showed that malaria remains a serious public health problem in the Shai Osudoku District Hospital, Ghana. The information obtained from this study can guide the implementation of malaria prevention, control and elimination strategies in Ghana.
Perihematomal edema after minimally invasive surgery: A matter of concern to Neurosurgeon
Authors: Lei Pan , Li Zhiyang , Song Ping , Wei Hangyu , Gao Lun , Zhou Long , Cheng Li , Hua Qiuwei , Wang Wenju , Cai Qiang 
Published: March 2023

Abstract
Background and purpose
To explore the evolution of brain edema after minimally invasive surgery (MIS) in deep spontaneous cerebral hemorrhage (DSICH) treatment and to analyze the differences in edema after different surgical methods.
Methods
The clinical data of 92 patients with DSICH treated in Renmin Hospital of Wuhan University from January 2020 to December 2021 were analyzed retrospectively. Among them, 47 cases were treated with minimally invasive puncture and drainage surgery (MIPDS group), and 45 were treated with neuroendoscopic surgery (NES group). The continuous Computed Tomography (CT) images of patients in the hospital and 3D-Slicer software were used to quantitatively calculate the edema area to explore the changes in perihematomal edema(PHE) volume in the two groups after the operation.
Results
The peak volume of postoperative edema (37.68 ± 10.74 mL) in the MIPDS group was more extensive than that of the NES group, and the net increase in edema volume was 17.47 ± 10.15 mL more than that of the NES group. The relative edema index (0.87 ± 0.27) was lower in the NES group than in the MIPDS group. (P < 0.05). The peak of postoperative edema in the MIPDS group was more than that in 6–8 days after the operation, and that in the NES group was more than that in 3–5 days after the operation.
Conclusion
There are differences in perihematomal edema of DSICH treated by different minimally invasive methods. Compared with MIPDs group, NEs group showed earlier peak of cerebral edema and lower degree of cerebral edema. The absolute regression volume of edema in the MIDPs group was greater than that in the NEs group, but there was no difference in the regression rate of edema between the two groups.
Aggressive Progression to EGFR Tyrosine Kinase inhibitors in Advanced NSCLC Patients: Concomitant Mutations, Prognostic Indicator and Subsequent Management
Authors: Wen Ruishan , Chen Ying , Long Jinyu , Huang Xiulian , Guo Yuxin , Lin Baoquan , Yu Zongyang 
Published: March 2023

Abstract
Background—EGFR tyrosine kinase (TKIs) are recommend as the first-line treatment for non-small cell lung cancer (NSCLC) patients with EGFR mutation. However, some patients experience aggressive progression (PFS≤6 months) on the first-line EGFR TKI therapy. Therefore, our study is to analyze the potential influencing factors including clinical features, biomarkers, concomitant mutations et al.
Methods—A total of 1,073 NSCLC patients with EGFR mutation in a multi-center study from January 2019 to December 2021. The datum pathological and molecular characteristics were collected. The area under the receiver operating characteristic (ROC) curve was used to evaluate the predictive effect of Ki-67 on the first-line TKI. The curve of progression-free survival (PFS) was conducted by Kaplan-Meier method and tested by bilateral log-rank. Cox regression model was used to predict and evaluate PFS of different variables. Chi-square or Fisher analysis was used for correlation between groups.
Results—55 patients who show aggressive progression on the first-line TKI therapy were analyzed in this study, while 71 with slow progression (PFS＞6 months). Concomitant mutationsincluding AXIN2, P2CG and RAD51C mutations occurred only in the aggressively progressive group (P=0.029). Correlation between Ki-67 index and the aggressive progression of the first-line TKI therapy was significant statistically different (P<0.05). In the second-line therapy, the PFS of chemotherapy in combination with other treatments was better than single TKIs in the first ten months.
Conclusion—NSCLC harbored EGFR and concomitant mutations (such as AXIN2, PLCG2 and RAD51C), and / or Ki-67 high expression may indicate the aggressive progression to the first-line EGFR-TKI.
Community-based prevalence of intestinal schistosomiasis and associated risk factors in two endemic districts of Taiz governorate, Yemen
Authors: Al-Kabab Abdullnasser Ahmed , Sheikh Einas I. EL , Almikhlafi Abdulla A. S. 
Published: March 2023

Abstract
Schistosomiasis is still a major public health problem despite launching preventive chemotherapy campaigns through yearly treatment of school-age children since 2008.
Objectives: to identify prevalence, severity, and risk factors of intestinal schistosomiasis in Al-Shmaytin& Al-Mwaset districts-Taiz governorate's-Yemen's.
Methods: A cross sectional study using structured questionnaires to collect data: age, gender, marital status, education, income, location, occupation, behavioral features, duration of contact with water, clinical background, schistosomiasis awareness, and the results of stool investigation. SPSS version 24 used for statistical analysis.
Results: S. mansoni prevalence was (14.3%), a higher prevalence in Al-Mwaset 22.0% compared to Al-Shmaytin 6.9%. Most infections were of high intensity (43.3%). The overall geometric mean egg count was 241.5 EPG. AOR for: district 2.7 (95% CI: 0.94– 7.59), (p=0.06); receiving Praziquantel® 0.7 (95% CI: 0.28–1.63), (p=0.38); having dam near home 1.2 (95% CI: 0.43–3.55), (p=0.69); age 0.3 (95% CI: 0.11–1.03), (P= 0.05); having pool/ponds near home 4.2 (95% CI: 0.99–17.90), (p=0.05); defecate near water sources 2.6 (95% CI: 1.09– 6.33), (p=0.03), blood with stool 4.7 (95% CI: 0.08– 0.59), (p=0.03).
Conclusions: The residents in study areas were at moderate risk of developing intestinal schistosomiasis. The infection was more prevalent in Al-Mwaset, ages 6-18, living near pool/ponds or dam, defecate near water sources (has the main role), having blood with stool and did not receive Praziquantel®. The heavy intensity of infection was noted among: most of infected community, male, age of more than 18 years. It was recommended that, Praziquantel® be regularly given to communities.
Association of Estrogen Receptor 1 (ESR1) PvuII and XbaI polymorphisms and peripheral ESR1 mRNA levels with Alzheimer's disease
Authors: Guven Gamze , Koseoglu-Buyukkaya Pinar , Kılıc Melisa , Uzun Damla , Cavus Betul , Guclu-Geyik Filiz , Lohmann Ebba , Samanci Bedia , Gurvit Hakan , Hanagasi Hasmet , Bilgic Basar 
Published: March 2023

Abstract
Objectives
Estrogen receptor 1 (ESR1) gene polymorphisms are associated with Alzheimer's disease (AD) and ESR1 mRNA transcription is affected by polymorphisms in the first intronic region of the gene. ESR1 PvuII rs2234693 (NM 000125.3:c.453-397T > C) and XbaI rs9340799 (NM 000125.3:c.453-351A > G) polymorphisms have been shown to be associated with AD. In this study, we sought to determine the association of PvuII and XbaI polymorphisms with AD. We also examined whether PvuII and XbaI polymorphisms affect disease susceptibility by influencing ESR1 mRNA expression.
Methods
Genotyping was performed in 424 AD patients and 302 controls. The polymerase chain reaction and restriction enzyme digestion was used to determine the prevalence of the ESR1 polymorphisms. ESR1 mRNA expression was analyzed in blood cells of 85 patients and 53 age-matched controls by quantitative real-time polymerase chain reaction.
Results
Our results showed no significant difference in genotype and allele frequencies of ESR1 PvuII and XbaI polymorphisms between patients and controls but frequencies of the PvuII C and XbaI G alleles were significantly higher in patients with the APOE ε4 allele. ESR1 mRNA levels were significantly lower in AD patients compared with controls (p = 0.001). XbaI A allele is significantly associated with lower ESR1 mRNA levels (p = 0.044) and this association remained significant after adjusting for age, gender and APOE ε4 carrier status (p = 0.035).
Conclusion
Our study showed that the distribution of PvuII and XbaI alleles were associated with the APOE ε4 allele. The XbaI polymorphism may be associated with a higher risk of AD by altering ESR1 mRNA levels.
Thoracic Endovascular Aortic Repair for Haemolysis 17 Years after Aortic Arch Replacement with Elephant Trunk for Acute Aortic Dissection: A Case Report
Authors: Mitsuishi Atsuyuki , Miura Yujiro , Hirose Nobuyuki , Noguchi Tatsuya , Kawaguchi Juri , Okamoto Unpei 
Published: March 2023

Abstract
Background

The elephant trunk (ET) technique is a very useful surgical procedure; however, haemolysis in the aorta associated with ET has been previously reported. It normally occurs within several years after the surgery, and it is a rare case of rapidly progressing haemolysis 10 or more years after aortic arch replacement with ET.

Case presentation

A 53-year-old man with a history of Stanford type A aortic dissection (DeBakey type Ⅰ), who was treated with total arch aortic replacement and aorto-femoral bypass using a prosthetic graft 17 years ago, developed severe progressive haemolytic anaemia. The ET used for the initial surgery was narrowed, and mechanical haemolysis was suspected. We assumed that progressive mechanical haemolysis occurred because of degeneration of the prosthetic graft. Thoracic endovascular aortic repair was performed, and haemolysis and anaemia were mitigated postoperatively.

Conclusions

Haemolysis occurred 17 years after the initial surgery with ET. When haemolysis is suspected in a patient with ET, it must be identified as a cause of haemolysis even if 10 years or more have passed since the ET was inserted. To prevent this complication, attention should be paid to an appropriate ET length and diameter to avoid folding of the ET, particularly when the true cavity diameter is small.
A field bioassay for assessing ivermectin bio-efficacy in wild malaria vectors
Authors: Ominde Kelly M. , Kamau Yvonne , Karisa Jonathan , Muturi Martha N. , Kiuru Caroline , Wanjiku Caroline , Babu Lawrence , Yaah Festus , Tuwei Mercy , Musani Haron , Ondieki Zedekiah , Muriu Simon , Mwangangi Joseph , Chaccour Carlos , Maia Marta F. 
Published: March 2023

Abstract
Background: Ivermectin (IVM) mass drug administration is currently under evaluation as a complementary malaria vector control tool. Mosquitoes that ingest blood from treated hosts suffer reduction in survival. Estimating the bio-efficacy of IVM on wild-caught mosquitoes requires they ingest the drug in a bloodmeal either through a membrane or directly feeding on a treated host. The latter, has ethical implications, and the former results in very low feeding rates. Therefore, there is a need to develop a safe and effective method for monitoring IVM bio-efficacy in wild mosquitoes.
Results: The mosquitocidal effect of IVM when administered in glucose solution is not comparable to the observed effects when same concentrations are administered in blood. Equal concentrations of IVM administered in blood resulted in more pronounced reductions in the probability of mosquito survival compared to when administered in glucose solution only. However, by adding small amounts of blood to a glucose solution, mosquito mortality rates can be increased resulting in effects closer to what is observed when blood feeding. A field bioassay was developed in the insectary to test bio-efficacy of IVM by using glucose solution containing a small amount of blood and administering it through a filter paper. Finally, the feasibility of the bioassay was tested using wild-caught mosquitoes in Kwale, Kenya.
Conclusion: Bio-efficacy of ivermectin is strongly dependent on how the drug is ingested by the mosquito and is likely influenced by the associated digestive processes. The assay developed is a good candidate for field-based bio-efficacy monitoring: wild mosquitoes readily feed on the solution, the assay can be standardized using pre-selected concentrations and by not involving a treated blood host (human or animal) it avoids variation due to individual pharmacokinetic profiles as well as ethical issues. Meal volumes did not explain the difference between the lethality of IVM compared across the different meal types, further research is needed to understand the underlying mechanism.
Machine learning of cerebello-cerebral functional networks for mild cognitive impairment detection
Authors: Yao Qun , Qu Liangcheng , Song Bo , Wang Xixi , Wang Tong , Ma Wenying , Tian Minjie , Shen Bo , Qi Xingyang , Zhu Donglin , Lin Xingjian , Li Zonghong , Shi Jingping , Yin Kuiying 
Published: March 2023

Abstract

Background:
Early identification of degenerative processes in Alzheimer’s disease (AD) is essential. Cerebello-cerebral network changes can be used for early diagnosis of dementia and its stages, namely mild cognitive impairment (MCI) and AD.
Methods:
Features of cortical thickness (CT) and cerebello-cerebral functional connectivity (FC) extracted from MRI data were used to analyze structural and functional changes, and machine learning for the disease progression classification.
Results:
CT features have an accuracy of 92.05% for AD vs. HC, 88.64% for MCI vs. HC, and 83.13% for MCI vs. AD. Additionally, combined with convolutional CT and cerebello-cerebral FC features, the accuracy of the classifier reached 94.12% for MCI vs. HC, 90.91% for AD vs. HC, and 89.16% for MCI vs. AD, evaluated using support vector machines.
Conclusions:
The proposed pipeline offers a promising low-cost alternative for the diagnosis of preclinical AD and can be useful for other degenerative brain disorders.
Early urea-to-creatinine ratio to predict ICU-acquired weakness in critically ill patients with sepsis
Authors: Jiang Jie , Chen Hui , Meng Shan-shan , Pan Chun , Xie Jian-Feng , Guo Feng-Mei 
Published: March 2023

Abstract
Background
ICU-acquired weakness (ICUAW) is a serious and common complication of critical illness. Urea-to-creatinine ratio (UCR) reflects protein metabolism and is often used for the assessment of nutritional metabolic status. However, it has not been studied in ICUAW. This study aimed to investigate whether the UCR can predict the development of ICUAW in patients with sepsis.
Methods
Our retrospective observational study was performed in a university ICU from 2014 to 2021 on a group of adult (≥ 18 years) sepsis patients. We excluded patients who received renal replacement therapy in ICU or transferred from another ICU or the interval between twice upper abdominal CT scans༜5 days. The primary exposures were UCR on day 1, UCR on day 3 minus UCR on day 1(ΔUCR d3-d1). The primary outcome was the onset of ICUAW during hospitalization. We employed multivariate logistic regression models to explore the association between ΔUCR d3-d1 and ICUAW. We calculated the area under the receiver operating characteristic (AUROC) to see the predictive performance of UCR.
Results
A total of 482 patients were included in the final analysis. The median (IQR) age of the included patients was 62.00 (51.00, 74.00) years, with 350 males and 132 females. Among them, 271(56%) were diagnosed with ICUAW. Compared with the no ICUAW group, the UCR was lower on day 1 (0.09 [0.06–0.12] vs. 0.11 [0.08–0.15], P ≤ 0.001) in the ICUAW group, subsequently, it showed a significant increasing trend. In the ICUAW group, ΔUCR d3-d1 was significantly higher than in the no ICUAW group (2.04 [0.36–3.78] vs. -1.20 [-3.06–0.14], P < 0.001). Multivariate logistic regression analysis showed that ΔUCR d3-d1 was associated with an increased risk of ICUAW (Odds ratio, OR 1.40,95% CI:1.30–1.52). The predictive ability of ΔUCR d3-d1 for predictive ICUAW was 0.78[95% CI 0.74–0.83], adjusted of AUROC remains good (0.78[95% CI 0.75–0.83]). Subgroup analyses showed that in patients ever received neuromuscular blockers, the AUROC of the ΔUCR d3-d1 was significantly higher (0.96[95% CI 0.88–0.99] vs. 0.79[95% CI 0.74–0.83], P < 0.001).
Conclusion
ΔUCR d3-d1 is independently associated with ICUAW and can predict the development of ICUAW in patients with sepsis.