bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRiPPing the sensors: The osmosensing pathway of Polycystin 2
Authors: Márquez-Nogueras K.M. , Knutila R.M. , Vuchkosvka V. , Kuo IY. 
Published: May 2023

AbstractMutations to polycystin-2 (PC2), a non-selective cation permeant transient receptor potential channel, results in polycystic kidney disease (PKD). Despite the disease relevance of PC2, the physiological agonist that activates PC2 has remained elusive. As one of the earliest symptoms in PKD is a urine concentrating deficiency, we hypothesized that shifts in osmolarity experienced by the collecting duct cells would activate PC2 and loss of PC2 would prevent osmosensing. We found that mice with inducible PC2 knocked out (KO) in renal tubules had dilute urine. Hyperosmotic stimuli induced a rise in endoplasmic reticulum (ER)-mediated cytosolic calcium which was absent in PC2 KO mice and PC2 KO cells. A pathologic point mutation that prevents ion flux through PC2 inhibited the calcium rise, pointing to the centrality of PC2 in the osmotic response. To understand how an extracellular stimulus activated ER-localized PC2, we examined microtubule-ER dynamics, and found that the osmotically induced calcium increase was preceded by microtubule destabilization. This was due to a novel interaction between PC2 and the microtubule binding protein MAP4 that tethers the microtubules to the ER. Finally, disruption of the MAP4-PC2 interaction prevented incorporation of the water channel aquaporin 2 following a hyperosmotic challenge, in part explaining the dilute urine. Our results demonstrate that MAP4-dependent microtubule stabilization of ER-resident PC2 is required for PC2 to participate in the osmosensing pathway. Moreover, osmolarity represents abona fidephysiological stimulus for ER-localized PC2 and loss of PC2 in renal epithelial cells impairs osmosensing ability and urine concentrating capacity.Graphical Abstract
Ultrastructural analysis of prostate cancer tissue provides insights into androgen-dependent adaptations to membrane contact site establishment
Authors: Butler Lisa M. , Evergren Emma 
Published: May 2023

AbstractMembrane trafficking and organelle contact sites are important for regulating cell metabolism and survival; processes often deregulated in cancer. Prostate cancer is the second leading cause of cancer-related death in men in the developed world. While early-stage disease is curable by surgery or radiotherapy there is an unmet need to identify prognostic biomarkers, markers to treatment response and new therapeutic targets in intermediate-late stage disease. This study explored the morphology of organelles and membrane contact sites in tumor tissue from normal, low and intermediate histological grade groups. The morphology of organelles in secretory prostate epithelial cells; including Golgi apparatus, ER, lysosomes; was similar in prostate tissue samples across a range of Gleason scores. Mitochondrial morphology was not dramatically altered, but the number of membrane contacts with the ER notably increased with disease progression. A three-fold increase of tight mitochondria-ER membrane contact sites was observed in the intermediate Gleason score group compared to normal tissue. To investigate whether these changes were concurrent with an increased androgen signaling in the tissue, we investigated whether an anti-androgen used in the clinic to treat advanced prostate cancer (enzalutamide) could reverse the phenotype. Patient-derived explant tissues with an intermediate Gleason score were culturedex vivoin the presence or absence of enzalutamide and the number of ER-mitochondria contacts were quantified for each matched pair of tissues. Enzalutamide treated tissue showed a significant reduction in the number and length of mitochondria-ER contact sites, suggesting a novel androgen-dependent regulation of these membrane contact sites. This study provides evidence for the first time that prostate epithelial cells undergo adaptations in membrane contact sites between mitochondria and the ER during prostate cancer progression. These adaptations are androgen-dependent and provide evidence for a novel hormone-regulated mechanism that support establishment and extension of MAMs. Future studies will determine whether these changes are required to maintain pro-proliferative signaling and metabolic changes that support prostate cancer cell viability.
Structural basis of TRPV1 modulation by endogenous bioactive lipids
Authors: Arnold William R. , Mancino Adamo , Moss Frank R. , Frost Adam , Julius David , Cheng Yifan 
Published: May 2023

AbstractTRP ion channels are modulated by phosphoinositide lipids, but the underlying structural mechanisms remain unclear. The capsaicin- and heat-activated receptor, TRPV1, has served as a model for deciphering lipid modulation, which is relevant to understanding how pro-algesic agents enhance channel activity in the setting of inflammatory pain. Identification of a pocket within the TRPV1 transmembrane core has provided initial clues as to how phosphoinositide lipids bind to and regulate the channel. Here we show that this regulatory pocket can accommodate diverse lipid species, including the inflammatory lipid lysophosphatidic acid (LPA), whose actions are determined by their specific modes of binding. Furthermore, we show that an ‘empty pocket’ channel lacking an endogenous phosphoinositide lipid assumes an agonist-like state, even at low temperature, substantiating the concept that phosphoinositide lipids serve as negative TRPV1 modulators whose ejection from the binding pocket is a critical step towards activation by thermal or chemical stimuli.
Content moderation on social media: constructing accountability in the digital space
Authors: Clune Conor , McDaid Emma 
Published: May 2023

PurposeThe paper examines the content moderation practices and related public disclosures of the World's most popular social media organizations (SMOs). It seeks to understand how content moderation operates as a process of accountability to shape and inform how users (inter)act on social media and how SMOs account for these practices.Design/methodology/approachContent analysis of the content moderation practices for selected SMOs was conducted using a range of publicly available data. Drawing on seminal accountability studies and the concepts of hierarchical and holistic accountability, the authors investigate the design and appearance of the systems of accountability that seek to guide how users create and share content on social media.FindingsThe paper unpacks the four-stage process of content moderation enacted by the World's largest SMOs. The findings suggest that while social media accountability may allow SMOs to control the content shared on their platforms, it may struggle to condition user behavior. This argument is built around the limitations the authors found in the way performance expectations are communicated to users, the nature of the dialogue that manifests between SMOs and users who are “held to account”, and the metrics drawn upon to determine the effectiveness of SMOs content moderation activities.Originality/valueThis is the first paper to examine the content moderation practices of the World's largest SMOs. Doing so extends understanding of the forms of accountability that function in the digital space. Crucial future research opportunities are highlighted to provoke and guide debate in this research area of escalating importance.
Impact of COVID-19 on open universities worldwide: case studies from Asia, Africa and Europe
Authors: Hou Songyan 
Published: May 2023

PurposeThe outbreak of COVID-19 in 2020 has had a profound impact on education institutions at all levels. Open universities, with their privileged delivery method, have been in an advantageous position. In the earlier stages of the pandemic, they made remarkable contributions to assuring learning continuity. However, with more and more conventional universities migrating online, great changes have taken place in the field of higher education, and it is imperative for open universities to adjust their strategies in order to maintain their leading role in a technology-enabled education context. This paper aims to examine what challenges have been faced by open universities during the pandemic and how they will transform in the future.Design/methodology/approachSix open universities in Asia, Africa and Europe were selected as cases in this research to make a comparative study based on the papers in the volume beyond distance education [1]. Similarities and differences among the cases were analyzed in order to identify the developing trend for open universities in the international context.FindingsThe results showed that (1) open universities in these regions demonstrated their resilience in the pandemic; examples were that new technologies have been leveraged to implement totally online delivery with short notice and huge amount of learning resources were offered to the society. (2) However, they encountered challenges of delivering fully online examination due to the lockdown and quarantine policies, and open universities in African and the sole private institution suffered financial pressure due to improving information and communication technology infrastructure and staff training. Another challenge was the fierce competition from conventional universities that open universities in Asia and Europe came across. (3) Four main areas were identified for future development in order to respond to the challenges: No.1 is that programs such as health care, psychology, epidemiology, virology, immunology, data analytics, biology and bio-informatics have stimulated interest for African open universities to develop in the future; No. 2 is that open universities were seeking to innovate their teaching formats; short courses, such as micro credentials, might be developed as agile and flexible offerings which are expected to be suitable to learners in the pandemic context; No 3 is that programs and courses for upskilling in the context of digitalization will be implemented; and No. 4 is that lifelong learning is given a higher priority in order for open universities to stand securely in the higher education sector.Originality/valueThe study may give open university leaders a quick insight into their future development.
Work environment challenge and the wellbeing of women in construction industry in New Zealand – The mediating role of work morale
Authors: Rotimi Funmilayo Ebun , Brauner Marcela , Burfoot Megan , Naismith Nicola , Silva C.W. Chathurani , Mohaghegh Mahsa 
Published: May 2023

PurposeThis study aims to determine the effects of sexism and unfavourable job conditions on women's mental and physical wellbeing in construction operations. In addition, this study will establish the mediating role of work morale in the association between workplace challenges and wellbeing.Design/methodology/approachData for this study were obtained from women working in office environments, onsite professionals and those in trades. A total of 65 participants responded to the survey. The partial least square structural equation modelling method was applied to validate the measurement model and test the hypotheses.FindingsThe findings demonstrate that benevolent and hostile sexism directly influences work morale and indirectly affects women's mental and physical wellbeing in the construction industry. Hostile sexism is the most significant challenge for female construction workers in New Zealand.Research limitations/implicationsThe finding is theory-building and challenges the assumptions that the nature of the construction industry has a bearing on the mental and physical health of women in construction. In addition, the finding is useful for creating positive work environments that are both inclusive and empathetic to the diverse needs of the modern-day construction workforce. Thus, reducing the challenges experienced by construction women's workforce.Originality/valueThe current study focuses on women and those who identified themselves as such. The conclusions show the relevance of work morale to their mental wellbeing. This exploratory quantitative study contributes to research by advancing understanding of the challenges women face in the industry and the consequences of those challenges on their physical and mental wellbeing.
Luxury home market and pre-sales challenges: Aldeola-de-Siolim, Goa
Authors: Kar Pravat Surya 
Published: May 2023

Learning outcomes
The learning outcomes of this study are as follows: identify key elements of luxury branding in the context of a new residential real estate brand; select target segment/s and outline the sales pitch for a luxury residential real estate brand; plot the pre-sales stage of the customer journey path (CJP) for a luxury residential real estate brand; and plan a pre-sales customer engagement strategy for a luxury residential real estate brand.

Case overview/synopsis
This case enumerates Aldeola de Siolim, Goa’s (ASG) pre-sales promotional challenges. ASG was an upcoming luxury residential property in Goa, India. Venky Infar – the developer of ASG – a family-owned civil construction firm – wanted to diversify into Goa’s vibrant luxury housing market. In India’s housing market, the success of a project often depends on the “pre-sales,” i.e. attracting target customers and maximizing the sales before the construction. V. Rama Rao, the project manager’s task, was challenging because ASG and Venky were new entrants in a mature and competitive market. However, Rao was determined to capture a slice of this lucrative market. The case discusses the following four points to help the students understand the marketing challenges and decision context. First, ASG’s key attractions, second, overview of the Indian real estate market, third, characteristics of Goa’s luxury home market and finally, Customer Journey Path for residential real estate purchase. The case elaborates on the nuances of strategic dilemmas and and presents competitors' practices and emerging consumer trends.

Complexity academic level
The case will help students analyze and formulate a pre-sales promotional plan for a luxury real estate product. It is suitable for marketing elective courses, e.g. branding, sales management and luxury management.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 8: Marketing
Community college discourses of sustainability in an academic and professional corpus
Authors: Hou Minghui , Ayers David Franklin 
Published: May 2023

Purpose
The purpose of this study is to identify discourses of sustainability of community colleges and how they related to sustainability imaginaries.

Design/methodology/approach
This study used a combination of research strategies associated with corpus linguistics and critical discourse analysis. Data included 57 issues of Community College Journal, a professional magazine published by the American Association of Community Colleges, and 2,972 abstracts of dissertations about community colleges. Publication dates ranged from 2010 to 2020.

Findings
Community college discourse of sustainability coheres around six themes: careers and fields of study; curriculum and credentialing; campus ecological sustainability; administrative roles and processes; external organizations, partnerships and processes; and fiscal sustainability. There is little evidence of a sustainable living imaginary found.

Research limitations/implications
The analysis is limited to a specific set of professional and academic texts about community colleges. Future researchers should explore discourses of sustainability in other contexts.

Originality/value
There has been no research associated with critical discourse analysis and corpus linguistics to explore community college discourses of sustainability, specifically in the field of community college leadership. The findings of this study situate the community college within contests over sustainability competencies in the practice of community college leadership development.
Generating students’ loyalty towards the sustainable university during the later COVID-19 pandemic
Authors: Dabija Dan-Cristian , Campian Veronica , Stanca Liana , Tiron-Tudor Adriana 
Published: May 2023

Purpose
The COVID-19 pandemic has brought tremendous changes in society. Universities were among the few organisations with some previous knowledge of online education, being able to rapidly adapt by transferring already known best practices to the new context. As teaching moved to online, students encountered less sustainable implementation by their universities. This allowed the development of previously planned sustainable strategies so that when face-to-face teaching resumed, universities could be even more sustainable. This paper aims to explore loyalty to the sustainable university during the later COVID-19 pandemic based on the university’s efforts to manage a green campus.

Design/methodology/approach
To investigate loyalty towards the sustainable university during the later COVID-19 pandemic, a conceptual model is proposed. This research is grounded in an empirical investigation using a quantitative online survey implemented with online interviews, the relations between all latent constructs being analysed with SmartPLS.

Findings
The results show that university sustainability reflects student loyalty, outlining the image developed under the influence of green campus management. The results show that universities must intensify their efforts to support the sustainable agenda and create a sustainable academic brand, inducing student loyalty. The findings may attract the attention of other universities wishing to gain knowledge about the factors that students consider important in generating their loyalty.

Research limitations/implications
As this research was carried out in the later COVID-19 pandemic context from 2021, the students already had experience of online teaching, so their assessment of the sustainability strategies implemented could be quite different from their perceptions in the first months of the pandemic. This research provides a comprehensive insight into the overall strategy that a sustainable university might apply in a crisis context.

Social implications
Research has shown that green campus management has a positive impact on the sustainability of a university’s image, on how students perceive the university at which they study and on the university’s efforts to ensure efficient campus management. These factors contribute to the development of a strong and sustainable image of the university within the community.

Originality/value
The originality of this paper lies in the research questions designed to conceptualise and operationalise the generation of students' loyalty towards their university by encouraging and implementing sustainable strategies on campus. This paper highlights a structural model that combines strategic practices to determine students' loyalty towards a sustainable university during the COVID-19 pandemic.
The theory of second language development for international students
Authors: Liu Wei 
Published: May 2023

Purpose
Drawing on diverse findings in Second Language Acquisition (SLA) and Language Teaching research, but centering on the unique needs and contexts of international students in post-secondary education, this paper aims to develop a working theory of international students’ continued language development.

Design/methodology/approach
As a critical review, the paper focuses on the most relevant concepts that have important bearings on the research topic, such as attitude, motivation and willingness to communicate; the age and biological factor, namely, the critical period for SLA; the learning environment and methodological factors, such as the input hypothesis; and finally, the larger sociocultural factor, i.e. Bronfenbrenner’s Ecological Systems Theory of student development.

Findings
This paper has developed a comprehensive theory of second language development for international students by synthesizing all relevant research findings in SLA and language teaching research.

Research limitations/implications
Linguistic factors, i.e. how students’ different first languages impact their pace and difficulty in learning a second language, though important, are not included in this paper.

Practical implications
The paper can better inform international students, faculty members, support staff and even members of the larger community about the attributions, the processes and the possible outcomes of second language development for international students.

Originality/value
Second language development is an immensely important part of international students’ international education journey. But currently, there is no comprehensive and coherent understanding of this issue among stakeholders of international student success.
Sealing the gaps: enhancing quality control in business school dissertations
Authors: Kumar Atul , Gawande Amol , Agarwal Akash , Kale Shailendrakumar , Brar Vinaydeep , Raibagkar Shirish 
Published: May 2023

Purpose
The purpose of this study is to identify and address significant quality gaps present in business school dissertations in India. Dissertations, an integral and a special component of the overall business school learning process, acquaint students with the practical business world.

Design/methodology/approach
The authors benchmarked the dissertation processes of business schools in India against those of reputed business schools in Germany, France, Sweden and Australia. Using a survey questionnaire, the authors checked whether business schools in India followed international processes and, if not, what could be done to adopt such processes. A sample of 367 business schools was studied to obtain reasonable evidence.

Findings
There were major quality issues in the dissertation process across business schools in India. Serious groundwork preparation issues were identified, such as an absence of research proposals. Most business schools also reported that there were no rubrics for dissertation evaluation, resulting in high subjectivity in the evaluation process. Supervisor interactions and control over the progress of the dissertation were also found to be very weak. As a result, the authors conclude that dissertations from business schools in India have major gaps in quality.

Originality/value
This is a novel study that examines the quality assurance of business school dissertations. It highlights major quality concerns surrounding the business school dissertation process and suggests measures to address quality issues. The study’s implications apply to business schools in all developing countries and not just India.
Drones in hospitality and tourism: a literature review and research agenda
Authors: Sevilla-Sevilla Claudia , Mendieta-Aragón Adrián , Ruiz-Gómez Luis Manuel 
Published: May 2023

Purpose
Drones have become an important element within hospitality and tourism. The purpose of this study is to identify the corpus of knowledge and create a research agenda that establishes appropriate guidelines for future study of drone application in hospitality and tourism.

Design/methodology/approach
This work has been undertaken using a mixed-methods approach that combines quantitative and qualitative research and includes a review of the literature related to the study of drone use in hospitality and tourism.

Findings
The mixed-methods review identified gaps in the research, potential areas of study to enhance the scientific literature and potential uses of drones in tourism and hospitality for researchers, consumers and industry professionals.

Originality/value
This study makes an original contribution by establishing an integrated framework, which led to a synthesis of the research corpus and provided a holistic conceptualisation of the relationship between tourism and drones. In addition, the research agenda proposed will help boost and consolidate this emerging field of research.