bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
Authors: Nguyen Thi Huyen Thu , Nguyen Ngoc Anh Thơ , Nguyen Hai Dang , Nguyen Thi Thu Hien , Le Mai Huong , Pham Minh Quan , Do Huu Nghi , Hoang Kim Chi , Michalet Serge , Dijoux-Franca Marie-Geneviève , Pham Hoang Nam 
Published: February 2023

During the process of adapting to metal contamination, plants produce secondary metabolites that have the potential to modulate multidrug-resistant (MDR) phenotypes; this is achieved by inhibiting the activity of efflux pumps to reduce the minimum inhibitory concentrations (MICs) of antimicrobial substrates. Our study evaluated the effect of secondary metabolites of belowground parts of Pteris vittata L. and Fallopia japonica, two metal-tolerant plants from northern Vietnam, on six antibiotic-resistant Stenotrophomonas maltophilia strains possessing efflux pump resistance mechanisms that were isolated from soil and clinical samples. The chemical composition of aqueous and dichloromethane (DCM) fractions extracted from P. vittata and F. japonica was determined using UHPLC-DAD-ESI/QTOF analysis. The antibacterial and efflux pump inhibitory activities of the four fractions were evaluated for the six strains (K279a, 0366, BurA1, BurE1, PierC1, and 502) using a microdilution assay at fraction concentrations of 62.5, 125, and 250 μg/mL. The DCM fraction of F. japonica exhibited remarkable antibacterial activity against strain 0366, with a MIC of 31.25 μg/mL. Furthermore, this fraction also significantly decreased gentamicin MIC: four-fold and eight-fold reductions for BurA1 and BurE1 strains, respectively (when tested at 250 μg/mL), and two-fold and eight-fold reductions for K279a and BurE1 strains, respectively (when tested at 125 μg/mL). Pure emodin, the main component identified in the DCM fraction of F. japonica, and sennidine A&B only reduced by half the MIC of gentamicin (when tested at 30 μg/mL). Our results suggest that the DCM fraction components of F. japonica underground parts may be potential candidates for new bacterial efflux pump inhibitors (EPIs).
Meteorological Variables Forecasting System Using Machine Learning and Open-Source Software
Authors: Segovia Jenny Aracely , Toaquiza Jonathan Fernando , Llanos Jacqueline Rosario , Rivas David Raimundo 
Published: February 2023

The techniques for forecasting meteorological variables are highly studied since prior knowledge of them allows for the efficient management of renewable energies, and also for other applications of science such as agriculture, health, engineering, energy, etc. In this research, the design, implementation, and comparison of forecasting models for meteorological variables have been performed using different Machine Learning techniques as part of Python open-source software. The techniques implemented include multiple linear regression, polynomial regression, random forest, decision tree, XGBoost, and multilayer perceptron neural network (MLP). To identify the best technique, the mean square error (RMSE), mean absolute percentage error (MAPE), mean absolute error (MAE), and coefficient of determination (R2) are used as evaluation metrics. The most efficient techniques depend on the variable to be forecasting, however, it is noted that for most of them, random forest and XGBoost techniques present better performance. For temperature, the best performing technique was Random Forest with an R2 of 0.8631, MAE of 0.4728 °C, MAPE of 2.73%, and RMSE of 0.6621 °C; for relative humidity, was Random Forest with an R2 of 0.8583, MAE of 2.1380RH, MAPE of 2.50% and RMSE of 2.9003 RH; for solar radiation, was Random Forest with an R2 of 0.7333, MAE of 65.8105 W/m2, and RMSE of 105.9141 W/m2; and for wind speed, was Random Forest with an R2 of 0.3660, MAE of 0.1097 m/s, and RMSE of 0.2136 m/s.
Disease Occurrence in- and the Transferal of Zoonotic Agents by North American Feedlot Cattle
Authors: Koyun Osman Y. , Balta Igori , Corcionivoschi Nicolae , Callaway Todd R. 
Published: February 2023

North America is a large producer of beef and contains approximately 12% of the world’s cattle inventory. Feedlots are an integral part of modern cattle production in North America, producing a high-quality, wholesome protein food for humans. Cattle, during their final stage, are fed readily digestible high-energy density rations in feedlots. Cattle in feedlots are susceptible to certain zoonotic diseases that impact cattle health, growth performance, and carcass characteristics, as well as human health. Diseases are often transferred amongst pen-mates, but they can also originate from the environment and be spread by vectors or fomites. Pathogen carriage in the gastrointestinal tract of cattle often leads to direct or indirect contamination of foods and the feedlot environment. This leads to the recirculation of these pathogens that have fecal–oral transmission within a feedlot cattle population for an extended time. Salmonella, Shiga toxin-producing Escherichia coli, and Campylobacter are commonly associated with animal-derived foods and can be transferred to humans through several routes such as contact with infected cattle and the consumption of contaminated meat. Brucellosis, anthrax, and leptospirosis, significant but neglected zoonotic diseases with debilitating impacts on human and animal health, are also discussed.
Novel Seed Size: A Novel Seed-Developing Gene in Glycine max
Authors: Zhang Mingxia , Dong Rui , Huang Penghui , Lu Mingyang , Feng Xianzhong , Fu Yongfu , Zhang Xiaomei 
Published: February 2023

Soybean-seed development is controlled in multiple ways, as in many known regulating genes. Here, we identify a novel gene, Novel Seed Size (NSS), involved in seed development, by analyzing a T-DNA mutant (S006). The S006 mutant is a random mutant of the GmFTL4pro:GUS transgenic line, with phenotypes with small and brown seed coats. An analysis of the metabolomics and transcriptome combined with RT-qPCR in the S006 seeds revealed that the brown coat may result from the increased expression of chalcone synthase 7/8 genes, while the down-regulated expression of NSS leads to small seed size. The seed phenotypes and a microscopic observation of the seed-coat integument cells in a CRISPR/Cas9-edited mutant nss1 confirmed that the NSS gene conferred small phenotypes of the S006 seeds. As mentioned in an annotation on the Phytozome website, NSS encodes a potential DNA helicase RuvA subunit, and no such genes were previously reported to be involved in seed development. Therefore, we identify a novel gene in a new pathway controlling seed development in soybeans.
A Control Method for Water Cannon of Unmanned Fireboats Considering Wind and Ship Motion Disturbances
Authors: Gao Diju , Xie Weixi , Bao Chunteng , Liu Bin , Zhuang Jiaxing 
Published: February 2023

In order to realize accurate and fast firefighting at sea, a control method for water cannons of unmanned fireboats considering wind and ship motion disturbances is presented. This method combines information fusion, computer vision, and prediction technology based on neural network. Firstly, a prediction model of the jet trajectory of the fire water cannon considering the disturbances of constant horizontal wind is established, and the effective range of the water cannon’s angles under the target working environment is obtained. Secondly, fusing the visual recognition information and predicted ship motion attitudes information, a double adaptive fuzzy controller is designed to compensate for the disturbances caused by the change in ship motion attitudes. Meanwhile, the online particle swarm optimization (PSO) is applied to fuzzy control to improve operational accuracy while enhancing the ability to adapt to environmental changes. The proposed control method was experimentally verified. As a result, the adaptive fuzzy controller based on the PSO can self-adjust the parameters to adapt to the changes in the working environment within 0.6 s, and the efficiency is improved by about 20%∼50% compared with the traditional fuzzy control. The double adaptive fuzzy control can reach a stable and effective working state within 10 simulation steps, and the RMSE of the drop point error is only 3 ×10−3 m for 40 simulation steps after stabilization, which can effectively resist disturbances and improve efficiency and control accuracy. The control method can provide a practical reference for engineering applications of water cannon control of unmanned fireboats.
Robotic Single-Site Hysterectomy in Gynecologic Benign Pathology: A Systematic Review of the Literature
Authors: Riemma Gaetano , Pasanisi Francesca , Reino Antonella , Solazzo Maria Cristina , Ronsini Carlo 
Published: February 2023

Background and objectives: Total hysterectomy is one of the most common gynecologic surgical procedures and it is mainly performed for benign pathologies. The introduction of robotic single-site surgery (RSS) as an acceptable alternative to laparoendoscopic surgery combines the advantages of robotics with the aesthetic result of a single incision. This study aims to review the existing literature on a single-site robotic hysterectomy in patients with benign pathologies and verify its safety and feasibility. Materials and Methods: Following the recommendations in the preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement, FP and AR systematically screened the PubMed, Embase, and Scopus databases. No temporal or geographical limitation was discriminatory. Studies containing data about feasibility and safety were included. Results: From 219, only eight studies met the inclusion criteria, and a total of 212 patients were included with a mean patient age of 45.42 years old (range 28–49.5 years old) and a mean BMI of 25.74 kg/m2 (range 22–28.5 kg/m2). The mean presurgical time, including port placement and docking time, was 15.56 (range 3–30) minutes. Mean console time was reported in six studies and is 83.21 min (range 25–180 min). The mean operative time is 136.6 min (range 60–294 min) and the mean blood loss is 43.68 mL (range 15–300 mL). Only two patients in the total analyzed had intraoperative complications and no conversion to LPT occurred. The median hospital stay was 1.71 days (range 0.96–3.5 days). The postoperative complication rate was estimated at 1.4% (vaginal bleeding). Conclusions: Our review supports the safety and feasibility of robotic single-site hysterectomy for benign gynecological diseases.
Quantum Gate Generation in Two-Level Open Quantum Systems by Coherent and Incoherent Photons Found with Gradient Search
Authors: Petruhanov Vadim N. , Pechen Alexander N. 
Published: February 2023

In this work, we consider an environment formed by incoherent photons as a resource for controlling open quantum systems via an incoherent control. We exploit a coherent control in the Hamiltonian and an incoherent control in the dissipator which induces the time-dependent decoherence rates γk(t) (via time-dependent spectral density of incoherent photons) for generation of single-qubit gates for a two-level open quantum system which evolves according to the Gorini–Kossakowski–Sudarshan–Lindblad (GKSL) master equation with time-dependent coefficients determined by these coherent and incoherent controls. The control problem is formulated as minimization of the objective functional, which is the sum of Hilbert-Schmidt norms between four fixed basis states evolved under the GKSL master equation with controls and the same four states evolved under the ideal gate transformation. The exact expression for the gradient of the objective functional with respect to piecewise constant controls is obtained. Subsequent optimization is performed using a gradient type algorithm with an adaptive step size that leads to oscillating behaviour of the gradient norm vs. iterations. Optimal trajectories in the Bloch ball for various initial states are computed. A relation of quantum gate generation with optimization on complex Stiefel manifolds is discussed. We develop methodology and apply it here for unitary gates as a testing example. The next step is to apply the method for generation of non-unitary processes and to multi-level quantum systems.
Implementasi Konsep Wellness Architecture pada Amanjiwo
Authors: Suhartono Indra , Yohanes Basuki Dwisusanto  , P. Herman Wilianto  
Published: February 2023

Pengetahuan dan perhatian masyarakat terhadap kesehatan pada masa kini telah meningkat. Hal ini ditunjukan dengan peningkatan ketertarikan terhadap wellness dan produknya. wellness berarti kondisi kesehatan fisik, mental dan sosial yang baik dan bukan hanya sekedar terbebas dari penyakit. Pertumbuhan industri pariwisata wellness meningkat pesat jauh diatas industri pariwisata secara umum. Pertanyaan yang muncul adalah bagaimana hotel-hotel tersebut mengimplementasikan konsep wellness dalam bentuk fasilitas hotel secara fisik. Kajian mengenai arsitektur dan kaitannya dengan wellness dapat dikaji karena manusia hidup dalam ranah lingkungan binaan yang memiliki pengaruh terhadap kesehatan fisik, mental, dan sosial. Penelitian ini akan mengkaji tataan massa pada hotel yang memiliki kaitan dengan konsep wellness dan kaitan rancangan dengan wellness individu. Tiga hotel yang dimiliki oleh Aman grup yang ada di Indonesia akan menjadi kasus studi karena Aman Grup terkenal dengan wellness-nya. Hotel tersebut adalah Amanjiwo yang ada di Magelang. Hotel ini akan dibandingkan implementasi wellness mereka dari aspek tataan bangunan. Amanjiwo telah mendukung wellness dari segi visual, audial, termal, penciuman, dan kinestesia.
IDENTIFIKASI TUMBUHAN PAKU DI JALUR KAMPUNG EMPAT – KAMPUNG ENAM KOTA TARAKAN
Authors: Fitriani Fitriani , Aprianita Aprianita , Tandi Martina S. 
Published: February 2023

Tujuan penelitian ini adalah untuk melaksanakan pendataan jenis tumbuhan paku di daerah pemukiman warga di jalur Kampung Empat –Kampung Enam, Tarakan. Tumbuhan paku yang ditemukan sebanyak 11 spesies tumbuhan paku yang terdiri dari 5 famili. Famili terbanyak yang ditemukan adalah Polypodiaceae sebanyak 5 spesies. Kemudian famili terbanyak kedua adalah Lygodiaceae sebanyak 3 spesies. Famili selanjutnya adalah Aspleniaceae, Davalliaceae dan Pteridaceae, yang masing-masing ditemukan 1 spesies. Kata kunciTumbuhan Paku, Kampung Enam, Kampung Empat
PENENTUAN BILANGAN PENYABUNAN PADA LIMBAH BIODIESEL PERTAMINA DI TARAKAN
Authors: Fatmawati Fatmawati 
Published: February 2023

AbstrakPenelitian ini bertujuan untuk mengetahui nilai bilangan penyabunan pada sampel limbah biodiesel yang tak tercampurkan dan sampel limbah biodiesel yang dicampurkan dengan minyak kelapa dan minyak zaitun. Penentuan bilangan penyabunan ditentukan melalui metode destilasi refluks dan titrasi asidimetri yang kemudian dihitung berdasarkan rumus penentuan bilangan penyabunan. Hasil penelitian menunjukkan bahwa tidak berbeda jauh nilai bilangan penyabunan antara sampel limbah biodiesel yang tak tercampurkan dengan limbah biodiesel yang tercampurkan, nilai bilangan penyabunan sampel limbah biodiesel yang tak tercampurkan sebesar 230,2 mg KOH/g dan nilai bilangan penyabunan sampel limbah biodiesel yang tercampurkan sebesar 239 mg KOH/g. Kata kunciBiodiesel Pertamina, Bilangan Penyabunan, Limbah AbstractThe study aimed at identifying saponification number of unmixed sample of biodiesel waste and mixed samples of biodiesel waste. The sample in this study was obtained from PT. Pertamina in Tarakan. Saponification number were determined by refluks destilation method and acidimetry titration method. The data were analyzed using saponification number formula. The results of the study show that saponification number of unmixed sample of biodiesel waste and mixed samples of biodiesel waste not significant different. Saponification number of unmixed sample of biodiesel waste is 230,2 mg KOH/g and Saponification number of mixed samples of biodiesel waste is 239 mg KOH/g.Keywords Pertamina biodiesel, saponification number, waste
STUDI ETNOZOOLOGI SEJARAH PENGGUNAAN PATUNG KUDA (Equus caballus) SEBAGAI IKON KOTA KUNINGAN, JAWA BARAT
Authors: Prihandini Agnita , Umami Muhimatul 
Published: February 2023

AbstrakKota Kuningan yang terletak di kaki timur Gunung Ciremai ini memang identik dengan hewan Kuda, yang hingga kini dijadikan sebagai ikon dan lambang Pemerintah Kabupaten Kuningan. Maka dari itu, penelitian ini bertujuan untuk mengetahui hal apa saja yang melatarbelakangi penggunaan patung kuda sebagai Ikon Kota Kuningan serta untuk mengetahui makna dan filosofi adagium mengenai kuda yang konon melambangkan masyarakat Kuningan. Penulis mencoba melakukan suatu riset yang dikemas dalam suatu studi etnozoologi. Adapun metode yang digunakan dalam penelitian ini yaitu dengan pendekatan kualitatif melalui observasi, wawancara, serta diperkuat dengan studi literatur. Kemudian, hasil yang didapatkan dilanjutkan dengan analisis data secara deskriptif. Hasil penelitian menunjukkan bahwa peresmian patung kuda sebagai ikon kota kuningan dilatarbelakangi oleh aksi heroik Kuda bersejarah dengan nama si Windu dan Sang Empunya yakni Pengeran Adipati Ewangga yang sangat berperan dalam sejarah berdirinya kota ini. Pengkajian dari perspektif etnozoologi ini diharapkan dapat meningkatkan rasa kesadaran masyarakat Kuningan akan pentingnya menghargai jasa para pahlawan tanah airnya, lebih dari itu, agar meningkatkan solidaritas masyarakat untuk tetap melestarikan Kuda (Equus caballus) di Kabupaten Kuningan dengan manajemen pemeliharaan yang baik, sebab hingga kini populasinya kian menurun.Â Kata kunciEtnozoologi, Sejarah, Ikon Kota, Equus caballusÂ AbstractKuningan, which is located at the eastern foot of Mount Ciremai, is indeed synonymous with horses. So far, the horse has been used as an icon and symbol of the Kuningan Regency Government. Therefore, this study aims to find out behind the use of the horse statue as an icon of Kuningan. Besides, this study aims to find out the meaning and adage philosophy about the horse as a symbol of the Kuningan community. The author tried to do research that is packaged in an ethnozoological study. The method used in this study is a qualitative approach through observation, interviews and strengthened by literature studies. Then, the results obtained were continued with descriptive data analysis. The results showed that the horse statue as an icon was inspired by the heroic action of the historic horse "Windu" and its owner. The owner is Pangeran Adipati Ewangga, who played a crucial role in the founding of this city. This study from an ethnozoological perspective expected to increase the Kuningan Community's awareness about the importance of appreciating the services of the heroes of their homeland. More than that, to increase community solidarity to continue preserving the Horse (Equus caballus) in Kuningan Regency with good maintenance management, the population has been decreasing.Kata kunciEtnozoologi, Sejarah, Ikon Kota, Equus caballus
PENERAPAN MODEL PAKEM UNTUK MENINGKATKAN HASIL BELAJAR IPA DI SMP NEGERI 10 TARAKAN
Authors: Wahab Abdul , Zulfadli Zulfadli , Vlorensius Vlorensius 
Published: February 2023

Penelitian ini bertujuan untuk meningkatkan hasil belajar IPA dengan menerapkan model PAKEM Untuk Meningkatkan Hasil Belajar Di SMP Negeri 10 Tarakan. Jenis penelitian ini adalah penelitian Tindakan Kelas (PTK) yang terdiri atas dua siklus. Pada tiap siklus terdiri atas dua kali pertemuan, dengan tahapan pada setiap siklus yaitu perencanaan, tindakan, observasi dan refleksi. Instrument yang digunakan dalam penelitian ini yaitu, lembar observasi aktivitas guru, aktivitas siswa, Tes Hasil Belajar, dan Lembar Kerja siswa. Hasil penelitian menunjukan model pembelajaran PAKEM dapat meningkatkan hasil belajar IPA siswa kelas VII Di SMP Negeri 10 Tarakan. Pada siklus I 50% dan siklus II mengalami peningkatan yaitu 79%. Nilai siswa secara klasikal yang diperoleh tersebut telah mencapai indikator ketuntasan yaitu 75%. Hasil pengamatan aktivitas guru mengajar siklus I dengan skor 71% dengan kategori baik, siklus II dengan skor 74% masuk kedalam kategori baik dan hasil aktivitas siswa siklus I memperoleh persentase 64% untuk pertemuan 1 dengan kategori cukup dan pada pertemuan kedua memperoleh persentase 76% dengan kategori baik, siklus II pada pertemuan I memperoleh persentase 80% dan pertemuan kedua memperoleh persentase 84% masuk dalam berkategori sangat baik.Kata Kunci : Model Pembelajaran PAKEM, Hasil Belajar IPA This study aims to improve science learning outcomes by applying the PAKEM model to improve learning outcomes at SMP Negeri 10 Tarakan. This type of research is Classroom Action Research (CAR) which consists of two cycles. Each cycle consists of two meetings, with stages: planning, action, observation, and reflection. The instruments used in this study are teacher activity observation sheets, student activities, learning outcomes tests, and student worksheets. The results showed that the PAKEM learning model could improve science learning outcomes for seventh-grade students at SMP Negeri 10 Tarakan. In the first cycle, 50% and the second cycle increased by 79%. The classical student scores obtained have reached the completeness indicator, namely 75%. The results of observations of teacher teaching activities in cycle I with a score of 71% with a good category, cycle II with a score of 74% fall into the good category and the results of student activity in cycle I get a percentage of 64% for meeting 1 with a good category and at the second meeting a percentage of 76% with good category, cycle II at the first meeting obtained a percentage of 80% and the second meeting obtained a percentage of 84% included in the very good category.Keywords: PAKEM Learning Model, Science learning outcomes
ISOLASI DAN IDENTIFIKASI BAKTERI PENGURAI SULFIDA DARI LUMPUR MANGROVE HUTAN LINDUNG ANGKE KAPUK
Authors: Lestari Eni , Darnaedi Dedy , Ragamustari Safendrri Komara 
Published: February 2023

AbstrakMangrove memiliki mikroorganisme salah satunya bakteri. Lumpur mangrove memiliki kandungan hidrogen sulfida. Bakteri aerobik memetabolisme hidrogen sulfida ini menjadi senyawa sulfat. Isolasi menggunakan medium Thiosulfat Mineral Medium. Isolat yang diamati secara makroskopis. Isolat dipilih dari hasil pengamatan mikroskopik, uji katalase dan uji motilitas. Isolat diuji juga kinerja penurunan sulfida. Isolat dengan kinerja penurunan sulfida terbaik dilanjutkan untuk uji sekuensing 16S rRNA. Hasil sekuensing menunjukkan isolat dari lumpur mangrove yang memiliki kinerja penurunan sulfida terbaik dengan nilai 30,58% adalah bakteri spesies Bacillus aryabhattaiKata kunci : mangrove, sulfida,bakteri, aerobik, BacillusAbstractMangroves have a diversity of microorganisms, oneÂ of which is bacteria. Mangrove mud contains hydrogen sulfide. Aerobic bacteria metabolize this hydrogen sulfide to sulfate compounds. Isolation used thiosulfate mineral medium. The growing isolates observed macroscopically. Selected isolates from microscopic observation, catalase test and motility test. The isolates also tested for their sulfide reduction performance. The isolates with the best sulfide reduction performance continued for 16S rRNA sequencing assay. The best sulfide reduction performance is 30,58% and the bacteria species based result sequencing is Bacillus aryabhattaiÂ Keywords: mangrove, sulfide, bacteria, aerobic, BacillusÂ