bluuebunny/crossref_metadata_2025_split huggingface dataset:
The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes
Authors: Liu Dan , Xing Ruinan , Zhang Quanyu , Tian Xiaoxiang , Qi Yanping , Song Haixu , Liu Yanxia , Yu Haibo , Zhang Xiaolin , Jing Quanmin , Yan Chenghui , Han Yaling 
Published: September 2023

AbstractAutophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.
CircFam190a: a critical positive regulator of osteoclast differentiation via enhancement of the AKT1/HSP90β complex
Authors: Chen Kun , Chen Xi , Lang Chuandong , Yuan Xingshi , Huang Junming , Li Zhi , Xu Mingyou , Wu Kerong , Zhou Chenhe , Li Qidong , Zhu Chen , Liu Lianxin , Shang Xifu 
Published: September 2023

AbstractThe identification of key regulatory factors that control osteoclastogenesis is important. Accumulating evidence indicates that circular RNAs (circRNAs) are discrete functional entities. However, the complexities of circRNA expression as well as the extent of their regulatory functions during osteoclastogenesis have yet to be revealed. Here, based on circular RNA sequencing data, we identified a circular RNA, circFam190a, as a critical regulator of osteoclast differentiation and function. During osteoclastogenesis, circFam190a is significantly upregulated. In vitro, circFam190a enhanced osteoclast formation and function. In vivo, overexpression of circFam190a induced significant bone loss, while knockdown of circFam190a prevented pathological bone loss in an ovariectomized (OVX) mouse osteoporosis model. Mechanistically, our data suggest that circFam90a enhances the binding of AKT1 and HSP90β, promoting AKT1 stability. Altogether, our findings highlight the critical role of circFam190a as a positive regulator of osteoclastogenesis, and targeting circFam190a might be a promising therapeutic strategy for treating pathological bone loss.
Nacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila
Authors: Yamada Takayuki , Yoshinari Yuto , Tobo Masayuki , Habara Okiko , Nishimura Takashi 
Published: September 2023

AbstractProtein homeostasis (proteostasis) is crucial for the maintenance of cellular homeostasis. Impairment of proteostasis activates proteotoxic and unfolded protein response pathways to resolve cellular stress or induce apoptosis in damaged cells. However, the responses of individual tissues to proteotoxic stress and evoking cell death program have not been extensively explored in vivo. Here, we show that a reduction in Nascent polypeptide-associated complex protein alpha subunit (Nacα) specifically and progressively induces cell death inDrosophilafat body cells.Nacαmutants disrupt both ER integrity and the proteasomal degradation system, resulting in caspase activation through JNK and p53. Although forced activation of the JNK and p53 pathways was insufficient to induce cell death in the fat body, the reduction ofNacαsensitized fat body cells to intrinsic and environmental stresses. Reducing overall protein synthesis by mTor inhibition orMinutemutants alleviated the cell death phenotype inNacαmutant fat body cells. Our work revealed that Nacα is crucial for protecting the fat body from cell death by maintaining cellular proteostasis, thus demonstrating the coexistence of a unique vulnerability and cell death resistance in the fat body.
Development of the reactive force field and silicon dry/wet oxidation process modeling
Authors: Noaki Junichi , Numazawa Satoshi , Jeon Joohyun , Kochi Shuntaro 
Published: September 2023

AbstractWe developed the Si/O/H reactive force field parameter set and applied to silicon dry/wet oxidation process to understand the underlying physics of the thermal oxidation of the Si(100) surface. Through a systematic development of the Si/O parameter using the experimental data of the volumes of the SiO2 crystal as a reference, we reproduced the same result over the temperature range of 300–1300 K. With strategic tunings of parameters related to Si/O/H system, we carried out the simulation comparing the wet-and dry processes. A significant acceleration of the oxide film growth seen in the ‘in-situ-steam-generation (ISSG)’ is successfully represented. These properties of our model imply its applicability in wider scope. We compare our results with those obtained by using parameter set recently developed by other authors. Investigating the configuration of atoms near the interface of the SiO2 film, our model is found to be able to study the role of hydrogen atoms for the ISSG acceleration.
Alkynylated and triazole-linked aroyl-S,N-ketene acetals: one-pot synthesis of solid-state emissive dyes with aggregation-induced enhanced emission characteristics
Authors: Biesen Lukas , Hartmann Yannic , Müller Thomas J. J. 
Published: September 2023

AbstractAlkynylated aroyl-S,N-ketene acetals are readily synthesized in mostly excellent yields by a Sonogashira reaction resulting in a substance library of more than 20 examples. Upon expansion of the reaction sequence by deprotection and concatenating of the copper-click reaction in a one-pot fashion, a library of 11 triazole-ligated aroyl-S,N-ketene acetals is readily accessible. All derivatives show pronounced solid-state emission and aggregation-induced emission properties depending on the nature of the alkynyl or the triazole substituents.
Genipin versus Ferric Chloride cross-linked unmodified Gum Arabic/Chitosan/nano-Hydroxyapatite nanocomposite hydrogels as potential scaffolds for bone regeneration
Authors: Makar Lara E. , Nady Norhan , Shawky Neivin , Kandil Sherif H. 
Published: September 2023

AbstractFerric chloride (FeCl3) and Genipin were utilized as cross-linkers to create two types of nanocomposite hydrogels through physical and covalent cross-linking methods, respectively. The hydrogels were composed of unmodified Gum Arabic (GA), Chitosan (Ch), and natural nano-Hydroxyapatite (nHA) using an acrylic acid solvent. Both the natural nHA and the FeCl3 vs. genipin cross-linked GA/Ch/nHA nano-composite hydrogels were prepared and characterized using various in vitro and in vivo analysis techniques. The use of FeCl3 and genipin cross-linkers resulted in the formation of novel hydrogels with compressive strengths of (15.43–22.20 MPa), which are comparable to those of natural cortical bone. In vivo evaluation was conducted by creating calvarial defects (6 mm) in Sprague–Dawley male rats. The results showed the formation of new, full-thickness bone at the implantation sites in all groups, as evidenced by digital planar tomography and histological staining with Hematoxylin and Eosin stain (H & E). Additionally, the use of genipin as a cross-linker positively affected the hydrogel's hydrophilicity and porosity. These findings justify further investigation into the potential of these nanocomposite hydrogels for bone regeneration applications.
Hybrid quantum systems with high-T$$_c$$ superconducting resonators
Authors: Velluire-Pellat Z. , Maréchal E. , Moulonguet N. , Saïz G. , Ménard G. C. , Kozlov S. , Couëdo F. , Amari P. , Medous C. , Paris J. , Hostein R. , Lesueur J. , Feuillet-Palma C. , Bergeal N. 
Published: September 2023

AbstractSuperconducting microwave resonators are crucial elements of microwave circuits, offering a wide range of potential applications in modern science and technology. While conventional low-T$$_c$$

c

superconductors are mainly employed, high-T$$_c$$

c

cuprates could offer enhanced temperature and magnetic field operating ranges. Here, we report the realization of $$\textrm{YBa}_2\textrm{Cu}_3\textrm{O}_{7-\delta }$$

YBa
2

Cu
3

O

7
-
δ

superconducting coplanar waveguide resonators, and demonstrate a continuous evolution from a lossy undercoupled regime, to a lossless overcoupled regime by adjusting the device geometry, in good agreement with circuit model theory. A high-quality factor resonator was then used to perform electron spin resonance measurements on a molecular spin ensemble across a temperature range spanning two decades. We observe spin-cavity hybridization indicating coherent coupling between the microwave field and the spins in a highly cooperative regime. The temperature dependence of the Rabi splitting and the spin relaxation time point toward an antiferromagnetic coupling of the spins below 2 K. Our findings indicate that high-Tc superconducting resonators hold great promise for the development of functional circuits. Additionally, they suggest novel approaches for achieving hybrid quantum systems based on high-T$$_c$$

c

superconductors and for conducting electron spin resonance measurements over a wide range of magnetic fields and temperatures.
Nrf2 inhibition increases sensitivity to chemotherapy of colorectal cancer by promoting ferroptosis and pyroptosis
Authors: Huang Yongzhou , Yang Wenchang , Yang Lei , Wang Tao , Li Chengguo , Yu Jiaxian , Zhang Peng , Yin Yuping , Li Ruidong , Tao Kaixiong 
Published: September 2023

AbstractOxaliplatin is widely used in chemotherapy for colorectal cancer (CRC), but its sensitivity has become a major obstacle to limiting efficacy. Many literatures reported that Nrf2 activation promoted tumor chemoresistance. In this study, we explored the role and mechanism of Nrf2 inhibition in oxaliplatin-based chemosensitivity of CRC. In vitro experiments, we applied 4-octyl itaconate (4-OI) to activate Nrf2, and used lentivirus to knock down Nrf2 in CRC cell lines. By measuring cell viability, colony formation, apoptosis, reactive oxygen species production, and western blot, we found that oxaliplatin and lobaplatin suppressed the growth of HCT-116 and LOVO cells in a dose-dependent manner, and promoted the expression of Nrf2. 4-OI, an Nrf2 activator, reduced the sensibility of CRC cells to oxaliplatin and lobaplatin, while the knockdown of Nrf2 promoted the sensibility of CRC cells to oxaliplatin and lobaplatin. Through the public databases, we found that the expression of GPX4 in normal tissues was lower compared with cancer tissues in CRC, and the high GPX4 expression predicted a poor prognosis. Meanwhile, we found that oxaliplatin reduced the expression of GPX4 in vitro. The knockdown of Nrf2 enhanced the effects of oxaliplatin to reduce the expression of GPX4 and GSH content, and increase the MDA content, which enhanced oxaliplatin-induced ferroptosis. Subsequently, we found that oxaliplatin promoted the expression of GSDME-N, and induced LDH, IL-1β, and TNF-a release, and the knockdown of Nrf2 aggravated the occurrence of GSMDE-mediated pyroptosis. Finally, we found that the knockdown of Nrf2 enhanced the inhibition of oxaliplatin on HCT116 xenograft tumor growth in vivo. Thus, our study showed that Nrf2 inhibition improved sensitivity to oxaliplatin of CRC cells by promoting ferroptosis and pyroptosis, which provided a new target for overcoming chemoresistance in CRC.
Fast, facile and thermal damage free nanowelding of Ag nanowire for flexible transparent conductive film by pressure-assisted microwave irradiation
Authors: Jeong Jong-Min , Sohn Minjeong , Bang Junghwan , Lee Tae-Ik , Kim Min-Su 
Published: September 2023

AbstractA fast and straightforward fabrication process for producing a robust, flexible, and transparent conductive film was demonstrated using nanowelding of Ag nanowires through pressure-assisted microwave irradiation. This innovative process effectively reduces the sheet resistance of the Ag nanowire transparent conductive film without causing any thermal distortion to the PET substrate. The microwave irradiation induces nanowelding between Ag nanowires, leading to a decrease in sheet resistance by forming nanowelding junctions. This selective heating of Ag nanowires further enhances the reduction in sheet resistance. Additionally, the application of pressure-assisted microwave irradiation allows the Ag nanowires to be embedded into the PET substrate, resulting in the formation of a robust film capable of withstanding cycling bending stress. The pressure-assisted microwave irradiation process proves to be a strong fabrication method for creating Ag nanowire transparent conductive films, especially when dealing with thermally weak substrate materials.
Iron-catalyzed oxosulfonylation of alkynes with small-ring compounds and Na2S2O5 for the synthesis of β-keto sulfones
Authors: Li Liu-Bin , Qiu Hui , Li Mu-Han , Dai Nan-Nan , Mei Lan , He Yu , Yu Lei , Li Ting , Wei Wen-Ting 
Published: September 2023

An efficient and rapid iron-catalyzed oxosulfonylation of alkynes with small-ring compounds and Na2S2O5 for the synthesis of β-keto sulfones has been developed.
Activated Clotting Time (ACT): The Reproducibility of Three Techniques
Authors: Sillick Paul V. , Amiot Daniel M. , Pancner George 
Published: September 2023

(J. Extra-Corpor. Technol. 19[3] p. 265-267 Fall 1987, 6 ref.) The ACT has routinely been used to monitor anticoagulation during surgical procedures, including open heart surgery. Since there is no laboratory substrate that can serve as a control for the test, it is important to know the reproducibility of the ACT. This study examined the reproducibility of three ACT techniques using the single syringe method.
Before heparinization, a 7cc sample of blood was collected from each of 50 open heart surgery patients. From this sample two Hemochron ACTs (H-ACT), two Sonoclot Coagulation ACTs (SonACT), and two manual ACTs (M-ACT) were simultaneously performed. The mean ± S.D. (seconds) was found to be 105.6 ± 31.8 for the SonACT, 109.7 ± 27.2 for the H-ACT, and 126.8 ± 15.4 for theM-ACT. Five minutes after heparinization the ACTs showed means of 485.5 ± 93.3 for the Son ACT, 455.6 ± 1 01.9 for the HACT, and 471.2 ± 84.9 for the M-ACT.
The absolute value of the difference between the paired observations, a function of the reproducibility, was seen to have a mean of 8.8 ± 13.4 for the SonACT, 6.1 ± 7.0 for the H-ACT, and 8.3 ± 7.6 for theM-ACT. Post heparinization, the absolute difference between the paired observations gave means of 90.7 ± 99.7 for the SonACT, 56.7 ± 59.8 for the HACT, and 40.4 ± 45.4 for the M-ACT.
Before heparinization, all three techniques gave acceptable results. With heparinization the M-ACT showed the most reproducibility, followed by the H-ACT, while the SonACT provided the least consistent results.
Evaluation of Three Types of Membrane Oxygenators and Their Suitability for Use with Pulsatile Flow
Authors: Gassmann Craig J. , Galbraith George D. , Smith Ricky G. 
Published: September 2023

(J. Extra-Corpor. Technol. 19[3] p. 297-304 Fall 1987, 26 ref.) Three types of single-pumphead arterial line microporous polypropylene membrane oxygenators were evaluated: 1) flat plate; 2) hollow fiber with blood path inside the fibers; and 3) hollow fiber with blood path outside the fibers. These oxygenator types were evaluated to determine what hemodynamic effects they would have on the pulse waveform in the extracorporeal arterial line and the radial artery monitoring line of the patient during pulsatile cardiopulmonary bypass. Gaseous microemboli (GME) generated by these oxygenators during pulsatile cardiopulmonary bypass were also measured.
Hemodynamically, the flat plate type exhibited the greatest tendency to dampen the pulsatile waveform because it is the most compliant membrane of the three types evaluated. The hollow fiber with blood path inside the fibers type had the highest line pressures proximal to the membrane and low pressure distal to the membrane because it is the most resistant to flow (i.e., high resistance across the membrane). The hollow fiber with blood path outside the fibers type had the least effect on the transmission of the pulsatile waveform because it has very low resistance across the membrane and very little compliance.
With regard to gaseous microemboli, the flat plate type and the hollow fiber with blood path outside the fibers type had negligible counts, during both continuous flow and pulsatile flow. The hollow fiber with blood path inside the fibers type had somewhat elevated gaseous microemboli counts, especially during initiation of pulsatile cardiopulmonary bypass; however, these diminished with time.
We conclude that the hollow fiber with blood path outside the fibers type is the type oxygenator of choice when employing pulsatile cardiopulmonary bypass with an arterial line membrane oxygenator.
Pulmonary Artery to Descending Thoracic Aorta Bypass: An Alternative Approach to Vascular Access for Open Heart Surgery
Authors: Gil William J. , Lansing Allan M. , Gornet Patricia H. 
Published: September 2023

(J. Extra-Corpor. Technol. 19[3] p. 372-375 Fall 1987, 8 ref.) A variety of conditions can increase the mortality and morbidity of a midsternotomy approach for cardiothoracic surgery. Among these complicating conditions are reoperations, sternal infections and past mediastinal radiation therapy. In the face of the inherent risks of midsternotomy in these situations an alternate, left thoracotomy approach, has successfully been employed.
The left thoracotomy approach for cardiopulmonary bypass necessitates a unique cannulation technique. The pulmonary artery is cannulated for venous return to the cardiopulmonary bypass circuit. Systemic blood flow to the patient is via the descending thoracic aorta. The femoral artery may also be used for arterial access.
The physiological principles that allow the pulmonary artery to be used as the venous cannulation site, the cardiothoracic procedures this may avail itself to, and the safety techniques used when this approach is employed will be discussed. Several cases in which this technique was used will also be reviewed.
Surveying the Research Interests, Needs and Productivity of Perfusion Educators
Authors: Bennett Denise E. , Beckley Philip D. 
Published: September 2023

(J. Extra-Corpor. Technol. 19[3] p. 384-391 Fall 1987, 5 ref.) The continuing advances in perfusion have brought an increased need for research to validate professional roles and practices. Perfusion educators are faced with increased expectations that they will conduct this research even though many feel they do not have the background or support to do so.
In an effort to address these concerns, a national study was done to determine the research interests, needs, skills, and productivity of educators in the field of perfusion. The goal of the study was 1) to determine the level of involvement in research activities of perfusion faculty; 2) to identify research interests of these educators; 3) to characterize the current research environment; 4) to determine the research background of perfusion educators; and 5) to identify continuing education needs for research productivity.
Twenty-seven perfusion education programs were contacted for lists of their current faculty members. A survey questionnaire was mailed to the 63 names received. Completed surveys were received from 26 individuals (41%). The overall results of the survey indicate low research productivity, a lack of research skills and support for research, and a need for workshops dealing with the development of research skills.
CT im Kindes- und Jugendalter
Authors: Tsiflikas Ilias 
Published: September 2023

ZusammenfassungCT-Untersuchungen von Kindern und Jugendlichen sind trotz aller Weiterentwicklungen der CT-Technik immer noch eine Herausforderung. Themen dieses Beitrags sind die Vorbereitung dieser
Untersuchungen, dedizierte pädiatrische CT-Protokolle mit dem Hauptfokus auf der Verringerung der Strahlendosis und typische CT-Indikationen.
Predictors of success of conventional ERCP for bile duct stones and need for single-operator cholangioscopy
Authors: El Menabawey Tareq , Mulay Akhilesh , Graham David , Phillpotts Simon , Sethi Amrita , Webster George J 
Published: September 2023

Abstract
Background and study aims The characteristics of difficult stones requiring cholangioscopy-assisted lithotripsy are poorly defined. We sought to determine clinician perception of these characteristics and decision-making in biliary endoscopy.
Methods One hundred twenty-four delegates attending an online
course were invited to assess 20 clinical stone cases. Each image was graded on a 4-point
Likert for: grading of stone difficulty, confidence of clearance with conventional endoscopic
retrograde cholangiopancreatography (ERCP) methods, likelihood of needing
cholangioscopy-assisted lithotripsy, and confidence of clearance with one session of
lithotripsy. An independent reviewer rated each case on largest stone size, stone number,
presence of stricture distal to stone, size of stone relative to distal duct size, and acute
common bile duct (CBD) angulation < 135°. Multilevel (mixed) statistical methods with a
two-level model were utilized with multilevel ordinal logistic regression.
Results Stone size and location, stricture and stone
diameter:duct ratio impacted perceived procedural difficulty P <
0.01). Stone:duct ratio (< 50% odds ratio [OR] 0.22, P <
0.001), stricture (OR 7.26, P < 0.001) and stone location
impacted confidence of clearance with conventional ERCP. Intrahepatic and cystic duct stones
were least likely to engender confidence (P < 0.01). The same
factors plus CBD angulation < 135° predicted cholangioscopy requirement (P < 0.01). Stone number did not influence procedure difficulty or
cholangioscopy requirement. Strictures (OR 0.29, P < 0.001) and
location, especially intrahepatic (OR 0.42, P < 0.001) impaired
confidence in clearance with one cholangioscopy session.
Conclusions Ductal anatomy, the presence of a stricture distal to a stone, cystic and intrahepatic stones and stones larger than the distal duct are considered by endoscopists to be significant predictors of requiring cholangioscopy-assisted lithotripsy.