bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Perioperative Complications After Anterior–Posterior Versus Posterior–Anterior–Posterior Cervical Fusion: A Retrospective Review of 153 Consecutive Cases
Authors: Bakare Adewale A. , Varela Jesus R. , Moss Jonah R , Platt Andrew , O'Toole John E. , Fontes Ricardo B. V. , Traynelis Vincent C. 
Published: March 2023

BACKGROUND:
Although published data support the utilization of circumferential fusion to treat select cervical spine pathologies, it is unclear whether the posterior–anterior–posterior (PAP) fusion has increased risks compared with the anterior–posterior fusion.

OBJECTIVE:
To evaluate the differences in perioperative complications between the 2 circumferential cervical fusion approaches.

METHODS:
One hundred fifty-three consecutive adult patients who underwent single-staged circumferential cervical fusion for degenerative pathologies from 2010 to 2021 were retrospectively reviewed. Patients were stratified into the anterior–posterior (n = 116) and PAP (n = 37) groups. The primary outcomes assessed were major complications, reoperation, and readmission.

RESULTS:
Although the PAP group was older (P = .024), predominantly female (P = .024), with higher baseline neck disability index (P = .026), cervical sagittal vertical axis (P = .001), and previous cervical operation rate (P < .00001), the major complication, reoperation, and readmission rates were not significantly different from the 360° group. Although the PAP group had higher urinary tract infection (P = .043) and transfusion (P = .007) rates, higher estimated blood loss (P = .034), and longer operative times (P < .00001), these differences were insignificant after the multivariable analysis. Overall, operative time was associated with older age (odds ratio [OR] 17.72, P = .042), atrial fibrillation (OR 158.30, P = .045), previous cervical operation (OR 5.05, P = .051), and lower baseline C1-7 lordosis (OR 0.93, P = .007). Higher estimated blood loss was associated with older age (OR 1.13, P = .005), male gender (OR 323.31, P = .047), and higher baseline cervical sagittal vertical axis (OR 9.65, P = .022).

CONCLUSION:
Despite some differences in preoperative and intraoperative variables, this study suggests both circumferential approaches have comparable reoperation, readmission, and complication profiles, all of which are high.
Stereotactic Radiosurgery for Vestibular Schwannoma in Neurofibromatosis Type 2: An International Multicenter Case Series of Response and Malignant Transformation Risk
Authors: Bin-Alamer Othman , Faramand Andrew , Alarifi Norah A. , Wei Zhishuo , Mallela Arka N. , Lu Victor M. , Nabeel Ahmed M. , Reda Wael A. , Tawadros Sameh R. , Abdelkarim Khaled , El-Shehaby Amr M.N. , Emad Reem M. , Peker Selcuk , Samanci Yavuz , Lee Cheng-chia , Yang Huai-che , Delabar Violaine , Mathieu David , Tripathi Manjul , Kearns Kathryn Nicole , Bunevicius Adomas , Sheehan Jason P. , Chytka Tomas , Liscak Roman , Moreno Nuria Martínez , Álvarez Roberto Martínez , Grills Inga S. , Parzen Jacob S. , Cifarelli Christopher P. , Rehman Azeem A. , Speckter Herwin , Niranjan Ajay , Lunsford L. Dade , Abou-Al-Shaar Hussam 
Published: March 2023

BACKGROUND:Vestibular schwannomas (VSs) related to neurofibromatosis type 2 (NF2) are challenging tumors. The increasing use of stereotactic radiosurgery (SRS) necessitates further investigations of its role and safety.OBJECTIVE:To evaluate tumor control, freedom from additional treatment (FFAT), serviceable hearing preservation, and radiation-related risks of patients with NF2 after SRS for VS.METHODS:We performed a retrospective study of 267 patients with NF2 (328 VSs) who underwent single-session SRS at 12 centers participating in the International Radiosurgery Research Foundation. The median patient age was 31 years (IQR, 21-45 years), and 52% were male.RESULTS:A total of 328 tumors underwent SRS during a median follow-up time of 59 months (IQR, 23-112 months). At 10 and 15 years, the tumor control rates were 77% (95% CI: 69%-84%) and 52% (95% CI: 40%-64%), respectively, and the FFAT rate were 85% (95% CI: 79%-90%) and 75% (95% CI: 65%-86%), respectively. At 5 and 10 years, the serviceable hearing preservation rates were 64% (95% CI: 55%-75%) and 35% (95% CI: 25%-54%), respectively. In the multivariate analysis, age (hazards ratio: 1.03 [95% CI: 1.01-1.05];P= .02) and bilateral VSs (hazards ratio: 4.56 [95% CI: 1.05-19.78];P= .04) were predictors for serviceable hearing loss. Neither radiation-induced tumors nor malignant transformation were encountered in this cohort.CONCLUSION:Although the absolute volumetric tumor progression rate was 48% at 15 years, the rate of FFAT related to VS was 75% at 15 years after SRS. None of the patients with NF2-related VS developed a new radiation-related neoplasm or malignant transformation after SRS.
Gamma Knife Stereotactic Radiosurgery for Trigeminal Neuralgia Secondary to Multiple Sclerosis: A Case-Control Study
Authors: Leduc William , Mathieu David , Adam Elizabeth , Ferreira Raphaëlle , Iorio-Morin Christian 
Published: March 2023

BACKGROUND:
The efficacy of stereotactic radiosurgery (SRS) for the relief of trigeminal neuralgia (TN) is well established. Much less is known, however, about the benefit of SRS for multiple sclerosis (MS)–related TN (MS-TN).

OBJECTIVE:
To compare outcomes in patients who underwent SRS for MS-TN vs classical/idiopathic TN and identify relative risk factors for failure.

METHODS:
We conducted a retrospective, case-control study of patients who underwent Gamma Knife radiosurgery at our center for MS-TN between October 2004 and November 2017. Cases were matched 1:1 to controls using a propensity score predicting MS probability using pretreatment variables. The final cohort consisted of 154 patients (77 cases and 77 controls). Baseline demographics, pain characteristics, and MRI features were collected before treatment. Pain evolution and complications were obtained at follow-up. Outcomes were analyzed using the Kaplan-Meir estimator and Cox regressions.

RESULTS:
There was no statistically significant difference between both groups with regards to initial pain relief (modified Barrow National Institute IIIa or less), which was achieved in 77% of patients with MS and 69% of controls. In responders, 78% of patients with MS and 52% of controls eventually had recurrence. Pain recurred earlier in patients with MS (29 months) than in controls (75 months). Complications were similarly distributed in each group and consisted, in the MS group, of 3% of new bothersome facial hypoesthesia and 1% of new dysesthesia.

CONCLUSION:
SRS is a safe and effective modality to achieve pain freedom in MS-TN. However, pain relief is significantly less durable than in matched controls without MS.
The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left–right asymmetry in the gut by promoting receptor endocytosis
Authors: Lai Yi-Ting , Sasamura Takeshi , Kuroda Junpei , Maeda Reo , Nakamura Mitsutoshi , Hatori Ryo , Ishibashi Tomoki , Taniguchi Kiichiro , Ooike Masashi , Taguchi Tomohiro , Nakazawa Naotaka , Hozumi Shunya , Okumura Takashi , Aigaki Toshiro , Inaki Mikiko , Matsuno Kenji 
Published: March 2023

ABSTRACT
Many organs of Drosophila show stereotypical left–right (LR) asymmetry; however, the underlying mechanisms remain elusive. Here, we have identified an evolutionarily conserved ubiquitin-binding protein, AWP1/Doctor No (Drn), as a factor required for LR asymmetry in the embryonic anterior gut. We found that drn is essential in the circular visceral muscle cells of the midgut for JAK/STAT signaling, which contributes to the first known cue for anterior gut lateralization via LR asymmetric nuclear rearrangement. Embryos homozygous for drn and lacking its maternal contribution showed phenotypes similar to those with depleted JAK/STAT signaling, suggesting that Drn is a general component of JAK/STAT signaling. Absence of Drn resulted in specific accumulation of Domeless (Dome), the receptor for ligands in the JAK/STAT signaling pathway, in intracellular compartments, including ubiquitylated cargos. Dome colocalized with Drn in wild-type Drosophila. These results suggest that Drn is required for the endocytic trafficking of Dome, which is a crucial step for activation of JAK/STAT signaling and the subsequent degradation of Dome. The roles of AWP1/Drn in activating JAK/STAT signaling and in LR asymmetric development may be conserved in various organisms.
FGF signaling regulates salivary gland branching morphogenesis by modulating cell adhesion
Authors: Ray Ayan T. , Soriano Philippe 
Published: March 2023

ABSTRACT
Loss of FGF signaling leads to defects in salivary gland branching, but the mechanisms underlying this phenotype remain largely unknown. We disrupted expression of Fgfr1 and Fgfr2 in salivary gland epithelial cells and found that both receptors function coordinately in regulating branching. Strikingly, branching morphogenesis in double knockouts is restored by Fgfr1 and Fgfr2 (Fgfr1/2) knock-in alleles incapable of engaging canonical RTK signaling, suggesting that additional FGF-dependent mechanisms play a role in salivary gland branching. Fgfr1/2 conditional null mutants showed defective cell-cell and cell-matrix adhesion, both of which have been shown to play instructive roles in salivary gland branching. Loss of FGF signaling led to disordered cell-basement membrane interactions in vivo as well as in organ culture. This was partially restored upon introducing Fgfr1/2 wild-type or signaling alleles that are incapable of eliciting canonical intracellular signaling. Together, our results identify non-canonical FGF signaling mechanisms that regulate branching morphogenesis through cell-adhesion processes.
Myosin-X recruits lamellipodin to filopodia tips
Authors: Popović Ana , Miihkinen Mitro , Ghimire Sujan , Saup Rafael , Grönloh Max L. B. , Ball Neil J. , Goult Benjamin T. , Ivaska Johanna , Jacquemet Guillaume 
Published: March 2023

ABSTRACT
Myosin-X (MYO10), a molecular motor localizing to filopodia, is thought to transport various cargo to filopodia tips, modulating filopodia function. However, only a few MYO10 cargoes have been described. Here, using GFP-Trap and BioID approaches combined with mass spectrometry, we identified lamellipodin (RAPH1) as a novel MYO10 cargo. We report that the FERM domain of MYO10 is required for RAPH1 localization and accumulation at filopodia tips. Previous studies have mapped the RAPH1 interaction domain for adhesome components to its talin-binding and Ras-association domains. Surprisingly, we find that the RAPH1 MYO10-binding site is not within these domains. Instead, it comprises a conserved helix located just after the RAPH1 pleckstrin homology domain with previously unknown functions. Functionally, RAPH1 supports MYO10 filopodia formation and stability but is not required to activate integrins at filopodia tips. Taken together, our data indicate a feed-forward mechanism whereby MYO10 filopodia are positively regulated by MYO10-mediated transport of RAPH1 to the filopodium tip.
Multimorbidity research in Sub-Saharan Africa: Proceedings of an interdisciplinary workshop
Authors: Banda Gift T. , Bosire Edna , Bunn Christopher , Chandler Clare I.R. , Chikumbu Edith , Chiwanda Jonathan , Dixon Justin , Ferrand Rashida A. , Kengne Andre-Pascal , Limbani Felix , Mendenhall Emily , Morton Ben , Moshabela Mosa , Peer Nasheeta , Salimu Sangwani , Silman Alan , Simiyu Ibrahim G. , Spencer Stephen A. , Tamuhla Tsaone , Tiffin Nicki , Yongolo Nateiya M. ,   on behalf of the workshop contributors (listed in the acknowledgements)
Published: March 2023

As life expectancies rise globally, the number of people living with multiple chronic health conditions – commonly referred to as ‘multimorbidity’ – is rising. Multimorbidity has been recognised as especially challenging to respond to in countries whose health systems are under-funded, fragmented, and designed primarily for acute care, including in sub-Saharan Africa. A growing body of research in sub-Saharan Africa has sought to better understand the particular challenges multimorbidity poses in the region and to develop context-sensitive responses. However, with multimorbidity still crystallising as a subject of enquiry, there remains considerable heterogeneity in conceptualising multimorbidity across disciplines and fields, hindering coordinated action. In June 2022, 60 researchers, practitioners, and stakeholders with regional expertise from nine sub-Saharan African countries gathered in Blantyre, Malawi to discuss ongoing multimorbidity research across the region. Drawing on insights from disciplines including epidemiology, public health, clinical medicine, anthropology, history, and sociology, participants critically considered the meaning, singular potential, and limitations of the concept of multimorbidity in sub-Saharan Africa. The workshop emphasised the need to move beyond a disease-centred concept of multimorbidity to one foregrounding patients’ values, needs, and social context; the importance of foregrounding structures and systems rather than behaviour and lifestyles; the value of a flexible (rather than standard) definition of multimorbidity; and the need to leverage local knowledge, expertise, resources, and infrastructure. The workshop further served as a platform for exploring opportunities for training, writing, and ongoing collaboration.
Cryptic presentation of cryptococcal osteomyelitis in an apparently immunocompetent individual
Authors: Subramony Hariharan , Raghavan Balagurunathan , Subbaiyan Eswer , Hegde Manjunatha Gajanana , Ramanathan Kartik 
Published: March 2023

Cryptococcosis is an opportunistic fungal infection seen in immunocompromised individuals. It is caused by the yeast-like fungus Cryptococcus and predominantly affects the lungs and central nervous system. Immunocompetent individuals very rarely develop bone involvement and only a few cases of cryptococcal osteomyelitis in patients without other comorbid conditions have been described. Only one other case of pelvic involvement, which was accompanied by lymphopaenia, has been reported. We describe the case of a 42-year-old immunocompetent man with cryptococcal hip osteomyelitis.
Deep learning based image quality improvement of a light-field microscope integrated with an epi-fluorescence microscope
Authors: Nakatani Noriyuki , Shinke Ryo , Quan Xiangyu , Murata Takashi , Matoba Osamu 
Published: March 2023

Light-field three-dimensional (3D) fluorescence microscopes can acquire 3D fluorescence images in a single shot, and followed numerical reconstruction can realize cross-sectional imaging at an arbitrary depth. The typical configuration that uses a lens array and a single image sensor has the trade-off between depth information acquisition and spatial resolution of each cross-sectional image. The spatial resolution of the reconstructed image degrades when depth information increases. In this paper, we use U-net as a deep learning model to improve the quality of reconstructed images. We constructed an optical system that integrates a light-field microscope and an epifluorescence microscope, which acquire the light-field data and high-resolution two-dimensional images, respectively. The high-resolution images from the epifluorescence microscope are used as ground-truth images for the training dataset for deep learning. The experimental results using fluorescent beads with a size of 10 µm and cultured tobacco cells showed significant improvement in the reconstructed images. Furthermore, time-lapse measurements were demonstrated in tobacco cells to observe the cell division process.
Two-beam phase correlation spectroscopy: a label-free holographic method to quantify particle flow in biofluids
Authors: Yu Lan , Wang Yu , Wang Yang , Zhuo Kequn , Liu Min , Nienhaus G. Ulrich , Gao Peng 
Published: March 2023

We introduce two-beam phase correlation spectroscopy (2B-ΦCS) as a label-free technique to measure the dynamics of flowing particles; e.g., in vitro or in vivo blood flow. 2B-ΦCS combines phase imaging with correlation spectroscopy, using the intrinsic refractive index contrast of particles against the fluid background in correlation analysis. This method starts with the acquisition of a time series of phase images of flowing particles using partially coherent point-diffraction digital holographic microscopy. Then, phase fluctuations from two selected circular regions in the image series are correlated to determine the concentration and flow velocity of the particles by fitting pair correlation curves with a physical model. 2B-ΦCS is a facile procedure when using a microfluidic channel, as shown by the measurements on flowing yeast microparticles, polymethyl methacrylate microparticles, and diluted rat blood. In the latter experiment, the concentration and average diameter of rat blood cells were determined to be (4.7±1.9)×106  μL−1 and 4.6±0.4  μm, respectively. We further analyzed the flow of mainly red blood cells in the tail vessels of live zebrafish embryos. Arterial and venous flow velocities were measured as 290±110  μm s−1 and 120±50  μm s−1, respectively. We envision that our technique will find applications in imaging transparent organisms and other areas of the life sciences and biomedicine.
Ultrasound versus fluoroscopy as imaging guidance for percutaneous nephrolithotomy: A systematic review and meta-analysis
Authors: Arabzadeh Bahri Razman , Maleki Saba , Shafiee Arman , Shobeiri Parnian 
Published: March 2023

Objectives
To determine whether the outcomes of ultrasound-guided percutaneous nephrolithotomy (UG-PCNL), an alternative to traditional fluoroscopy-guided percutaneous nephrolithotomy (FG-PCNL), are comparable.

Methods
A systematic search of PubMed, Embase, and the Cochrane Library was carried out to discover investigations comparing UG-PCNL to FG-PCNL, and accordingly, a meta-analysis of those studies was performed. The primary outcomes included the stone-free rate (SFR), overall complications based on Clavien-Dindo classification, duration of surgery, duration of patients’ hospitalization, and hemoglobin (Hb) drop during the surgery. All statistical analyses and visualizations were implemented utilizing R software.

Results
Nineteen studies, including eight randomized clinical trials (RCTs) and eleven observational cohorts, comprising 3016 patients (1521 UG-PCNL patients) and comparing UG-PCNL with FG-PCNL met the inclusion criteria of the current study. Considering SFR, overall complications, duration of surgery, duration of hospitalization, and Hb drop, our meta-analysis revealed no statistically significant difference between UG-PCNL and FG-PCNL patients, with p-values of 0.29, 0.47, 0.98, 0.28, and 0.42, respectively. Significant differences were discovered between UG-PCNL and FG-PCNL patients in terms of the length of time they were exposed to radiation (p-value< 0.0001). Moreover, FG-PCNL had shorter access time than UG-PCNL (p-value = 0.04).

Conclusion
UG-PCNL provides the advantage of requiring less radiation exposure while being just as efficient as FG-PCNL; thus, this study suggests prioritizing the use of UG-PCNL.
The principles of recovery-oriented mental health services: A review of the guidelines from five different countries for developing a protocol to be implemented in Yogyakarta, Indonesia
Authors: Subandi M. A. , Nihayah Maryama , Marchira Carla R. , Tyas Trihayuning , Marastuti Ariana , Pratiwi Ratri , Mediola Fiddina , Herdiyanto Yohanes K. , Sari Osi Kusuma , Good Mary-Jo D. , Good Byron J. 
Published: March 2023

Background
Recovery-oriented mental health service has become the focus of global change in mental health services. Most of North industrialized countries have adopted and implemented this paradigm in the last two decades. Only recently that some developing countries are trying to follow this step. In Indonesia’s case, there has been little attention to developing a recovery orientation by mental health authorities. The aim of this article is to synthesize and analyze the recovery-oriented guidelines from five industrialized countries that we can use as a primary model for developing a protocol to be implemented in community health centre in Kulonprogo District, Yogyakarta, Indonesia.

Method
We used a narrative literature review by searching for guidelines from many different sources. We found 57 guidelines, but only 13 from five countries met the criteria, including five guidelines from Australia, one from Ireland, three from Canada, two from the UK, and two from the US. To analyze the data, we used an inductive thematic analysis to explore the themes of each principle as described by the guideline.

Result
The results of the thematic analysis revealed seven recovery principles, including (1) cultivating positive hope, (2) establishing partnerships and collaboration, (3) ensuring organizational commitment and evaluation, (4) recognizing the consumer’s rights, (5) focusing on person-centeredness and empowerment, (6) recognizing an individual’s uniqueness and social context, and (7) facilitating social support,. These seven principles are not independent, rather they are interrelated and overlap each other.

Conclusion
The principle of person-centeredness and empowerment is central to the recovery-oriented mental health system, while the principle of hope is also essential to embracing all the other principles. We will adjust and implement the result of the review in our project focusing on developing recovery-oriented mental health service in the community health center in Yogyakarta, Indonesia. We hope that this framework will be adopted by the central government in Indonesia and other developing countries.
The clinical characteristics, etiologic pathogens and the risk factors associated with dehydration status among under-five children hospitalized with acute diarrhea in Savannakhet Province, Lao PDR
Authors: Vorlasane Latdavanh , Luu Mai Ngoc , Tiwari Ranjit , Imoto Atsuko , Sato Miho , Huy Nguyen Tien , Kamiya Yasuhiko , Moji Kazuhiko 
Published: March 2023

BackgroundAcute diarrhea is a common health problem in children, especially those under five years of age (U5). The mortality rate due to acute diarrhea among U5 children accounted for 11% in Lao PDR in 2016. No study has been done to investigate the etiologic pathogens of acute diarrhea and the risk factors associated with dehydration status among U5 children hospitalized with acute diarrhea in this region.ObjectivesThe study aimed to evaluate the clinical characteristics, etiological agents and associated factors of dehydration status of acute diarrhea among hospitalized U5 children in Savannakhet Province, Lao PDR.MethodsThis retrospective study reviewed paper-based medical records with available stool examination results of 33 U5 children hospitalized with acute diarrhea in Savannakhet Provincial Hospital, Lao PDR between Jan. 2018 and Dec. 2019. Descriptive statistics were used to describe clinical characteristics and etiologic agents of acute diarrhea of the children. Nonparametric test, Pearson’s Chi-square test and Fisher exact test were used to determine the risk factors associated with level of dehydration of the participants.ResultsVomiting was the most common symptom (66.6%), followed by fever (60.6%). Dehydration was found in 48.4% of subjects. Rotavirus was the most common identified pathogen with a prevalence of 55.5%. Bacterial enteric infection was identified in 15.1% of patients. There is a significantly higher prevalence of dehydration among children with acute diarrhea caused by rotavirus compared to those with negative rotavirus testing (70.0% vs. 12.5%, p = 0.02).ConclusionsRotavirus was the most prevalent pathogen of acute diarrhea among U5 children. Pediatric patients with acute diarrhea caused by rotavirus had a higher prevalence of dehydration compared to those with negative rotavirus testing.