bluuebunny/crossref_metadata_2025_split huggingface dataset:
X chromosome inactivation skewing is common in advanced carotid atherosclerotic lesions in females and predicts secondary peripheral artery events
Authors: Buono Michele Filippo , Benavente Ernest Diez , Daniels Mark , Mol Barend M. , Mekke Joost M. , de Borst Gert J. , de Kleijn Dominique P. V. , Laan Sander W. van der , Pasterkamp Gerard , Onland-Moret Charlotte , Mokry Michal , Ruijter Hester M. den 
Published: March 2023

Abstract
Background and aim: Sex differences in atherosclerosis have been described with female plaques being mostly perceived as stable and fibrous. Sex-specific mechanisms such as mosaic loss of the Y chromosome in men have been linked to cardiovascular health. In women, X-linked mechanisms such as X chromosome inactivation (XCI) skewing is common in several tissues. Yet, information on the role of XCI in female atherosclerotic plaques is lacking. Here, we investigated the presence of XCI skewing in advanced atherosclerotic lesions and its association with cardiovascular risk factors, histological plaque data, and clinical data.
Methods: XCI skewing was quantified in 154 atherosclerotic plaque and 55 blood DNA samples of women included in the Athero-Express study. The skewing status was determined performing the HUMARA assay. Then, we studied the relationship of XCI skewing in female plaque and cardiovascular risk factors using regression models. In addition, we studied if plaque XCI predicted plaque composition, and adverse events during 3-years follow-up using Cox proportional hazard models.
Results: XCI skewing was detected in 76 of 154 (49.4%) plaques and in 27 of 55 (67%) blood samples. None of the clinical risk factors were associated with plaque skewing. Plaque skewing was more often detected in plaques with a plaque hemorrhage (OR [95% CI]: 1.44 [1.06 - 1.98], P = 0.02). Moreover, skewed plaques were not associated with a higher incidence of composite and major events but were specifically associated with peripheral artery events during a 3-year follow-up period in a multivariate model (HR [95%CI]: 1.46 [1.09 – 1.97]; P = 0.007).
Conclusions: XCI skewing is common in carotid plaques of females and is predictive for the occurrence of peripheral artery events within 3 years after carotid endarterectomy.
Timeline of lunar differentiation recorded in Ca and Mg stable isotopes
Authors: Fu Hairuo , Jacobsen Stein , Sedaghatpour Fatemeh 
Published: March 2023

Abstract
Stable isotopic variations recorded in lunar samples provide novel and crucial resolution to the differentiation history of the Moon. We report new high-precision Ca-isotope measurements for lunar rocks and minerals. Ca-isotope data and modeling of the lunar magma ocean together demonstrate indistinguishable Ca isotopic compositions between the bulk silicate Earth and Moon, consistent with isotope equilibration of the Earth and Moon preceding the lunar magma ocean differentiation. This is also consistent with the high-energy giant impact (Synestia) origin of the Earth-Moon system. Moreover, cross-comparison between Ca and Mg isotopic data for two anorthosite samples (60015 and 60025) consistently suggests their formation near the completion of lunar magma ocean crystallization (>99%) rather than reflecting early lunar crust as previously considered. Therefore, the various existing radiometric dating for 60025 sets the lunar magma ocean to have fully solidified by either 4.51 or 4.38 billion years ago, constraining the two respective lunar differentiation timescales to <30 (short-lived) or ~130–150 (long-lived) million years.
An inventory of human light exposure related behaviour
Authors: Siraji Mushfiqul Anwar , Lazar Rafael , Duijnhoven Juliëtte van , Schlangen Luc , Haque Shamsul , Kalavally Vineetha , Vetter Celine , Glickman Gena , Smolders Karin , Spitschan Manuel 
Published: March 2023

Abstract
Light exposure is an essential driver of health and well-being, and individual behaviours during rest and activity modulate physiologically-relevant aspects of light exposure. Further understanding the behaviours that influence individual photic exposure patterns may provide insight into the volitional contributions to the physiological effects of light and guide bevavioral points of intervention. Here, we present a novel, self-reported and psychometrically validated inventory to capture light exposure-related behaviour, the Light Exposure Behaviour Assessment (LEBA).
An expert panel prepared the initial 48-item pool spanning different light exposure-related behaviours. Responses, consisting of rating the frequency of engaging in the per-item behaviour on a 5-point Likert type scale, were collected in an online survey yielding responses from a geographically unconstrained sample (690 completed responses, 74 countries, 28 time zones). The exploratory factor analysis (EFA) on an initial subsample (n=428) rendered a five-factor solution with 25 items (Wearing blue light filters, spending time outdoors, using a phone and smartwatch in bed, using light before bedtime, using light in the morning and during daytime). In a confirmatory factor analysis (CFA) performed on an independent subset of participants (n=262), we removed two additional items to attain the best fit for the five-factor solution (CFI=0.95, TLI=0.95, RMSEA=0.06). The internal consistency reliability coefficient for the total instrument yielded McDonald’s Omega=0.68. Measurement model invariance analysis between native and non-native English speakers showed our model attained the highest level of invariance (residual invariance; CFI=0.95, TLI=0.95, RMSEA=0.05). Lastly, a short form of the LEBA (n=18) was developed using Item Response Theory on the complete sample (n=690).
The psychometric properties of the LEBA indicate the usability to measure light exposure-related behaviours. The instrument may offer a scalable solution to characterize behaviours that influence individual photic exposure patterns in remote samples. The LEBA inventory is available under the open-access CC-BY-NC-ND license.
Instrument webpage: https://leba-instrument.org/ GitHub repository containing this manuscript: https://github.com/leba-instrument/leba-manuscript
Association Between Trajectories of Prescription Opioid Use and Risk of Opioid Use Disorder and Overdose Among US Nonmetastatic Breast Cancer Survivors
Authors: Chang Ching-Yuan , Jones Bobby L. , Hincapie-Castillo Juan M. , Park Haesuk , Heldermon Coy D. , Diaby Vakaramoko , Wilson Debbie L. , Lo-Ciganic Wei-Hsuan 
Published: March 2023

Abstract
Purpose
To examine the association between prescription opioid use trajectories and risk of opioid use disorder (OUD) or overdose among nonmetastatic breast cancer survivors by treatment type.
Methods
This retrospective cohort study included female nonmetastatic breast cancer survivors with at least 1 opioid prescription fill in 2010–2019 Surveillance, Epidemiology and End Results linked Medicare data. Opioid mean daily morphine milligram equivalents (MME) calculated within 1.5 years after initiating active breast cancer therapy. Group-based trajectory models identified distinct opioid use trajectory patterns. Risk of time to first OUD/overdose event within 2.5 years after breast cancer therapy initiation was calculated for distinct trajectory groups using Cox proportional hazards models. Analyses were stratified by treatment type.
Results
Four opioid use trajectories were identified for each treatment group. For 38,265 survivors with systemic endocrine therapy, 3 trajectories were associated with increased OUD/overdose risk compared with early discontinuation: minimal dose (< 5 MME; adjusted hazard ratio [aHR] = 4.46 [95% CI = 3.09–6.43]), very low dose (5–25 MME; 15.60 [10.74–22.67]), and moderate dose (51–90 MME; 58.55 [39.92–85.86]). For 9,558 survivors with adjuvant chemotherapy, 3 trajectories were associated with higher OUD/overdose risks compared with early discontinuation: minimal dose (aHR = 3.80 [95% CI = 1.98–7.32]), low dose (26–50 MME; 11.66 [6.01–22.61]), and high dose (91–150 MME; 16.49 [5.90-46.09]). For 3,550 survivors with neoadjuvant chemotherapy, low-dose opioid use was associated with higher OUD/overdose risk (aHR = 5.60 [95% CI = 1.91–16.45]) compared with minimal-dose use.
Conclusions
Among Medicare nonmetastatic breast cancer survivors receiving systemic endocrine therapy or adjuvant chemotherapy, compared with early discontinuation, moderate-dose and high-dose opioid use were associated with 17- to 59-fold higher OUD/overdose risk. For females receiving neoadjuvant chemotherapy, low-dose opioid use was associated with 6-fold higher OUD/overdose risk compared with minimal-dose use. Breast cancer survivors at high-risk of OUD/overdose may benefit from targeted interventions (e.g., pain clinic referral).
Prognostic role of preoperative serum total cholesterol and high-density lipoprotein in lung adenocarcinoma
Authors: Li Zhupeng , Xu Jianfeng , Yu Guangmao , Zhou Junqing , Ma Zhifeng , Wu Yuanling , Zhu Ting , Dong Lingjun , Ding JianYi , Xu Haixia , Xu Peng 
Published: March 2023

Abstract
Objective: To evaluate the clinical significance of the preoperative plasma lipid profile, including triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), in patients with lung adenocarcinoma (LUAD) status post complete resection.
Methods: Clinical data, including preoperative plasma profile levels, were retrospectively collected and reviewed in 304 patients with LUAD who underwent radical lung resection between 2016–2017. The overall survival (OS) and disease-free survival (DFS) were assessed by the Kaplan–Meier method and the Cox proportional hazards regression model.
Results: TC ≤ 3.58 and HDL-C ≤ 1.01 were deemed as independent preoperative risk factors for OS, and HDL-C ≤ 1.01 was an independent preoperative risk factor for DFS. In the multivariate analyses involving OS and DFS, an increased TC level (HR, 0.504; 95% CI, 0.324–0.782, P = 0.002) was significantly associated with better OS. Additionally, a decreased HDL-C level was significantly associated with worse OS (HR, 0.665; 95% CI, 0.443–0.999, P = 0.049) and DFS (HR, 0.619; 95% CI, 0.420–0.912, P = 0.015).
Conclusions: In patients with LUAD, increased TC levels may predict better OS, while decreased levels of HDL-C may predict worse outcomes for both DFS and OS.
The underlying molecular mechanisms and biomarkers between periodontitis and COVID- 19
Authors: Qin Danlei , Yu Feiyan , Wu Dongchao , Han Chong , Yao Xuemin , Yang Lulu , Yang Xi , Wang Qianqian , He Dongning , Zhao Bin 
Published: March 2023

Abstract
Objective
Emerging evidence shows the clinical consequences of patient with COVID-19 and periodontitis are not promising, and periodontitis is a risk factor. Periodontitis and COVID-19 probably have a relationship. Hence, this study aimed to identify the common molecular mechanism that may help to devise potential therapeutic strategies in the future.
Material and methods
We analyzed two RNA-seq datasets for differential expressed genes, enrichment of biological processes, transcription factors (TFs) and deconvolution-based immune cell types in periodontitis, COVID-19 and healthy controls. Relationships between TFs and mRNA were established by Pearson correlation analysis, and the common TFs-mRNA regulatory network and nine co-upregulated TFs of the two diseases was obtained. The RT-PCR detected the TFs.
Results
A total of 1616 and 10201 DEGs from periodontitis and COVID-19 are found. Moreover, nine shared TFs and common biological processes associated with lymphocyte activation involved in immune response were identified across periodontitis and COVID-19. The cell type enrichment revealed elevated plasma cells among two diseases. The RT-PCR further confirmed the nine TFs up-regulation in periodontitis.
Conclusion
The pathogenesis of periodontitis and COVID-19 is closely related to the expression of TFs and lymphocyte activation, which can provide potential targets for treatment.
Assessing Land Degradation in Amansie West District: A Surface Mined Area in Ghana
Authors: Asiedu Rhoda Atiapa , Amaning Kwaku Adjei , Koomson Bennetta , Gyamfi Charles , Niang Awa 
Published: March 2023

AbstractThe Amansie West District is characterized by extensive land degrading activities. The district has become a nexus for vast illegal small-scale mining activities, thus affecting the sustainability of other land uses. It is thus imperative, that the land use land cover (LULC) trends over the years be monitored. This would aid assessment of changes over time and their future impacts on resources in the district. The study examined the LULC changes in the district with surface mining as a driver of change. Landsat images of 30m resolution for 1986, 2004, and 2015 were taken for Amansie West District in the Ashanti Region of Ghana. The images were classified using the Maximum Likelihood Classification. Verification was done using 200 ground truth points collected on-site and from Google Earth. The results showed changes in the Spatio-temporal distribution of LULC in the District over 30 years (1986–2015). The dominant LULC changes are from forest to farmland and farmland to mines for 30 years. In addition, there were losses from farmland to mines from 2004–2015. Farmland and forest were the dominant LULC types of the study area which made up 54.6% and 43.6% respectively for 1986, and likewise 54.4% and 22.3% for 2015. However, the forest cover was reduced drastically by 21.3% in 2015. The main drivers of land degradation were farmland from 1986–2004 and mines from 2004–2015. The study emphasizes the need for local-level studies by exposing the extent of the damage for effective future land use management.
miRNome profiling reveals differential miRNAs associated with embryogenic potential in the somatic embryogenesis of Araucaria angustifolia
Authors: de Oliveira Leandro Francisco , Piovezani Amanda Rusiska , Floh Eny Iochevet Segal 
Published: March 2023

Abstract
Somatic embryogenesis occurs through complex processes modulated by gene regulatory networks at an appropriate spatiotemporal scale important for cell division and differentiation. Post-transcription regulation mechanisms mediated by miRNAs control the expression of several genes involved in embryogenesis. Transcript and proteomics studies in embryogenic cultures from Araucaria angustifolia, an endangered native Brazilian conifer with ecological and economic importance, have indicated a role for post-transcriptional regulation in this process. One example is the differential abundance of ARGONAUTE between two contrasting embryogenic cell lines. Here, we profiled the miRNA expression pattern in two cell lines of A. angustifolia with distinct embryogenic potential using small RNA sequencing technology. We identified 165 mature miRNAs, of which 143 were novel and 22 were conserved plant miRNAs. Seven miRNA conserved families were identified: miR156, miR169, miR394, miR482, miR536, miR1030, and miR1314. Most miRNAs were differentially expressed during the transition from proliferation to the maturation stage of somatic embryogenesis, suggesting that miRNAs play more important roles in the early somatic embryo development. A total of 98 potential target genes were found for 89 miRNAs, involved in post-transcriptional processes, transporters, defense response, sugar regulation, stress, ABA controlling and signaling, cell-to-cell communication, maintaining suspensor cell identity, brassinosteroids signaling, and cell division. Negative correlations of expression patterns between miRNAs and their targets were detected for miR1030-PRL1, miR1314-ADR1-Like, and Aang34-LRR modules, when analyzed by RT-qPCR. Taken together, our findings provide new insights into the regulatory roles of miRNAs and their target genes in the somatic embryogenesis of Brazilian pine.
Optimized Energy Consumption by Optimal Routing Algorithm in Wireless Body Area Network
Authors: Duhan Nagendra , Malik Sanjay , Rathee Priyanka , Vyas Avani 
Published: March 2023

Abstract
Wireless Body Area Network (WBAN) is a relatively new form of wireless networking technology that has found many potential uses, particularly in the medical field, thanks to its ability to give users a sense of independence even when they are being closely monitored. Using tiny, low-power, battery-operated biosensors, WBAN is able to monitor and report on a wide range of physical characteristics of the person being studied. The difficulty in replacing or charging batteries during experiments is compounded by the fact that these sensors are implanted on or in the body being studied. Consequently, WBAN requires algorithms that can conserve energy. Since most of the energy consumed in such networks is due to communication and data transmission among the sensors, therefore routing algorithms aiming of energy conservation are required for WBAN. This paper proposes an optimal routing algorithm which optimizes the energy consumption of WBAN. The proposed routing algorithm focuses on two methodologies. First, the optimal distancing of forwarding node from both the sender node and the receiving node, and second, fragmentation of the whole network into sets with restriction based on a known threshold length till which single-hop data transmission without any data loss is possible. The proposed routing algorithm is simulated and analyzed compared to LEACH routing algorithm which uses the method of clustering in WBAN. The proposed routing algorithm outperforms in optimizing the energy consumption of WBAN and hence increasing the network lifetime.
Acoustic radiation of a simplified jet-flap-thrust gate configuration: numerical and experimental investigation
Authors: Sirotto José R. L. N. , Cordioli Julio A. , Nogueira Petrônio A. S. , Cavalieri André V. G. , Secchi Maicon , Wolf William R. 
Published: March 2023

Abstract
A comparative study of the acoustic far-field radiation of a subsonic jet near a folded plate with an opening, intended to represent a flapped wing with thrust gate, is presented in this work. Three openings with different widths were used to evaluate experimentally the influence of the gaps in the far-field noise radiation, for two folding angles. Boundary Element Method (BEM) simulations with a wavepacket model which represents the jet acoustic source are used to calculate the far-field noise. Numerical simulation results are compared with experimental measurements and show similar trends in terms of acoustic radiation. Through parametric simulations, it was also possible to estimate that opening widths greater than one jet diameter do not contribute significantly to reducing the far-field noise. The results show that even the smallest tested openings were able to reduce the far-field noise for the tested positions.
Sociospatial determinants of knowledge and perceptions of COVID-19 impacts among childbearing women in urban and rural areas of the Malagasy middle east
Authors: RAKOTOSAMIMANANA Sitraka , MANGAHASIMBOLA Reziky Tiandraza , RATOVOSON Rila , RANDREMANANA Rindra Vatosoa 
Published: March 2023

Abstract
Background
With regard to the coronavirus disease (COVID-19) pandemic in Madagascar, little is known about the knowledge and perceptions of and impacts on childbearing women. People's knowledge of COVID-19 can have an impact on their attitudes towards seeking care. The aim of the current study is to determine the knowledge of COVID-19 and associated determinants of childbearing women in Moramanga.
Methods
A cross-sectional study based on questionnaire administration was used in urban and rural areas of Moramanga, a district located 112 km from the capital, among childbearing women. Data collection was conducted from August to October 2021. A scoring method was applied to evaluate their knowledge level and perceptions about COVID-19 and its impacts on their lives. A binary stepwise logistic regression was performed to determine the sociodemographic and sociospatial determinants of their knowledge level towards COVID-19.
Results
A total of 885 childbearing women from urban and rural Moramanga areas were interviewed. Approximately 49.8% (441/885) lived in urban areas, and 50.2% (444/885) lived in rural areas. Approximately 35.3% (322/885) of the participants had a good level of knowledge of COVID-19. Multivariate analysis showed that the probability of having good level of knowledge of COVID-19 had a significant statistical association (p value < 0.05) with living in an urban area [AOR: 2.86; 95% CI (1.87–4.40)], telephone ownership [AOR: 1.71; 95% CI (0.16–1.1)], radio ownership [AOR 2.22; 95% CI (1.44–3.43)], watching TV [AOR = 1.95; 95% CI (1.34–2.83)] and reading journal paper [AOR = 3.74 95% CI (1.69–8.3)].
Conclusions
Levels of knowledge about COVID-19 among women of reproductive age in the middle east area of Madagascar are related to sociospatial determinants. Almost a third of the sample had a good level of knowledge of COVID-19. Access to information and telecommunication technologies increases the chances of being better informed about the disease. To avoid the negative repercussions of COVID-19 and infectious disease epidemics on maternal and child health, it is necessary to improve the awareness of childbearing women and other categories of the population about these diseases. This implies taking into account the geographic and demographic characteristics of the populations.
Health Workers' Knowledge on Maternal and Perinatal Deaths Surveillance and Response System and Its Associated Factors in Morogoro Region, Tanzania
Authors: Kashililika Christina , Millanzi Walter C. , Moshi Fabiola Vincent 
Published: March 2023

Abstract
The maternal and Perinatal Deaths Surveillance and Response (MPDSR) system is a technical system that was issued by the World Health Organization in 2013 to help developing countries improve maternal health. The major purpose of the system is to reduce the ongoing high numbers of maternal deaths and perinatal deaths from avertable causes. Tanzania adopted the MPDSR system in 2015. This study aimed to determine the level of knowledge on the MPDSR system and its associated factors among health workers in the Morogoro region. Method: It was an analytical cross-sectional study conducted from April 27, 2020, to May 29, 2020. A multi-stage sampling technique was used to recruit 360 health workers. A semi-structured questionnaire was used to collect data. Statistical package for social science (SPSS v.20) software was used for data entry and analysis. Bivariate and multivariate logistic regression was used to assess factors associated with knowledge about MPDSR. Results: A total of 105 (29.2%) health workers in the Morogoro Region had adequate knowledge of the MPDSR system.After controlling for confounders, variables that showed significant association with knowledge of health workers on the MPDSR system were the level of health facility a health worker was working [Hospital (AOR = 2.668 at 95% CI = 1.497–4.753, p = 0.001)], level of education of a health worker [Diploma (AOR = 0.146 at 95% CI = 0.038–0.561, p = 0.005)] and status of training on MPDSR [trained (AOR = 7.253 at 95% CI = 3.862–13.621, p = < 0.001)]. Conclusion: The proportion of health workers who had adequate knowledge about MPDSR system in Morogoro Region is unacceptably low. Factors that associated with adequate knowledge were those working in hospital with higher level of professional training and who had ever had training on MPDSR. A cost effective strategy to improve the level of knowledge about MPDSR in the region is highly recommended.