bluuebunny/crossref_metadata_2025_split huggingface dataset:
Testing for EGFR Variants in Pleural and Pericardial Effusion Cell-Free DNA in Patients With Non–Small Cell Lung Cancer
Authors: Lee Kirsty W. C. , Li Molly S. C. , Gai Wanxia , Lau Yat Ming , Chan Allen K. C. , Chan Oscar S. H. , Lee Chee Khoon , Yeung Rebecca M. W. , Fung Sherwood Y. H. , Cheung Wai F. , Chan Vivian W. , Leung Linda , Kam Kenny N. P. , Mok Tony S. K. 
Published: December 2022

ImportanceMolecular testing in non–small cell lung cancer (NSCLC) is commonly limited by inadequate tumor sample. Plasma cell-free DNA (cfDNA) genotyping as a complementary test is specific but only moderately sensitive. Genotyping of cfDNA in pleural and pericardial effusion (PE-cfDNA) can further optimize molecular diagnostic yield and reduce the need for repeated biopsies.ObjectiveTo prospectively validate droplet digital polymerase chain reaction (ddPCR) for detection of sensitizing EGFR variants and acquired Thr790Met variant (T790M) from PE-cfDNA in patients with NSCLC.Design, Setting, and ParticipantsThis prospective diagnostic validation study was conducted between September 6, 2016, and January 21, 2021 at 2 major Hong Kong cancer centers. Patients with advanced NSCLC with both wild-type and variant EGFR status and exudative PE who underwent thoracocentesis or pericardiocentesis were randomly enrolled. Patients were either EGFR-tyrosine kinase inhibitor (TKI) naive (cohort 1) or EGFR-TKI treated but osimertinib naive (cohort 2). Enrolled patients underwent pleural- or pericardial-fluid and blood sampling for ddPCR EGFR testing. EGFR status results with ddPCR testing of PE-cfDNA and blood were compared with EGFR status in matched tumor biopsy or PE cell block samples.Main Outcomes and MeasuresSpecificity, sensitivity, and concordance of PE-cfDNA for detection of sensitizing EGFR variants and acquired T790M variation.ResultsAmong 171 patients (54% female) enrolled, there were 104 in cohort 1 and 67 in cohort 2. In cohort 1, 37% (38/102) were EGFR-variant positive; PE-cfDNA showed 97% sensitivity (95% CI, 92%-100%), 97% specificity (95% CI, 93%-100%), and 97% concordance (ĸ = 0.94, P &amp;lt; .001) for the detection of sensitizing EGFR variants. It was more sensitive than plasma in detecting sensitizing EGFR variants (97% vs 74%, P &amp;lt; .001). In cohort 2, 38% (15 of 40) were positive for the EGFR T790M variant; PE-cfDNA showed 87% sensitivity (95% CI, 69%-100%), 60% specificity (95% CI, 41%-79%), and 70% concordance (ĸ = 0.42, P = .004) for acquired T790M. The EGFR T790M variant was detected in 51% of PE-cfDNA vs 25% of PE cell block samples.Conclusions and RelevanceIn this diagnostic study, EGFR variants could be accurately detected from PE-cfDNA in patients with NSCLC. More EGFR T790M was detected in PE-cfDNA than in guideline-recommended PE cell block preparations. These results suggest that PE-cfDNA can complement plasma and tumor genotyping for detecting EGFR variants in patients with advanced NSCLC.
An Integrated Solar Panel with a Triboelectric Nanogenerator Array for Synergistic Harvesting of Raindrop and Solar Energy
Authors: Ye Cuiying , Liu Di , Chen Pengfei , Cao Leo N. Y. , Li Xunjia , Jiang Tao , Wang Zhong Lin 
Published: December 2022

AbstractThe triboelectric nanogenerator (TENG) is regarded as an effective strategy for harvesting energy from raindrops, and is a complementary solution with solar cells to achieve all‐weather energy harvesting and sustainable energy supply. However, due to the irregularity of natural rainfalls in the volume, frequency, density, and location, designing high‐efficiency raindrop TENG (R‐TENG) arrays faces great challenges. In this work, a highly transparent, large‐area, and high‐efficiency R‐TENG array with rational material choice, electrode structure, and array distribution is developed for efficiently harvesting irregular raindrop energy. The problem of electrical signal cancellation among adjacent raindrops can be fully avoided, as viewed from the high‐resolution space–time analyses of high‐speed camera and electrical signal characteristics. With the rationally designed electrode instead of multiple complex electrodes, all charges can be exported by the R‐TENG array in a simulated irregular raindrop scenario. Moreover, it is demonstrated that the R‐TENG possesses higher average power density (40.80 mW m−2) than that of the solar cell (37.03 mW m−2) in rainy condition. Additionally, a self‐powered wireless light‐intensity‐monitoring system is demonstrated for real‐time and all‐day weather monitoring. This work provides useful guidance for designing high‐efficiency TENG arrays integrated with solar panels for harvesting irregular raindrop energy and solar energy.
Liquid‐Phase Synthesis of Highly Deformable and Air‐Stable Sn‐Substituted Li3PS4 for All‐Solid‐State Batteries Fabricated and Operated under Low Pressures
Authors: Woo Jehoon , Song Yong Bae , Kwak Hiram , Jun Seunggoo , Jang Bo Yeong , Park Juhyoun , Kim Kyu Tae , Park Changhyun , Lee Chanhee , Park Kern‐Ho , Lee Hyun‐Wook , Jung Yoon Seok 
Published: December 2022

AbstractThe liquid‐phase synthesis (LS) of sulfide solid electrolytes (SEs) has promising potential for mass production of practical all‐solid‐state Li batteries (ASLBs). However, their accessible SE compositions are mostly metal‐free. Moreover, liquid‐phase‐synthesized‐SEs (LS‐SEs) suffer from high electronic conductivity due to carbon impurities, resulting in below‐par electrochemical performance of ASLBs. Here, the LS of highly deformable and air‐stable Li3+xP1‐xSnxS4 (0.19 mS cm−1) using 1,2‐ethylene diamine‐1,2‐ethanedithiol with tetrahydrofuran is reported. A low heat‐treatment temperature (260 °C) prevents the carbonization of organic residues. Importantly, a remarkable enhancement in the deformability of LS‐SEs compared to that of conventional solid‐state‐synthesized SEs (SS‐SEs) is identified for the first time. LiNi0.7Co0.15Mn0.15O2 electrodes employing LS‐SEs in ASLBs significantly outperform those using SS‐SEs, notably when assembled under a low fabricating pressure (148 vs 370 MPa, e.g., capacity loss: 2 vs 41 mA h g−1) or tested under a low operating pressure (12 or 3 MPa), which is attributed to reduced electrochemo‐mechanical effects. Finally, when employing SEs that are exposed to air (dew point of −20 °C), LiNi0.7Co0.15Mn0.15O2 electrodes employing SEs with Sn‐substituted composition or prepared by LS exhibit significantly better capacity retention than conventional SEs with Sn‐free composition or prepared by SS (e.g., 92.2% for LS‐Li3.2P0.8Sn0.2S4 vs 32.5% for SS‐Li3PS4).
Long noncoding RNA 01534 maintains cancer stemness by downregulating endoplasmic reticulum stress response in colorectal cancer
Authors: Ichihara Momoko , Takahashi Hidekazu , Nishida Naohiro , Ivan Cristina , Okuzaki Daisuke , Yokoyama Yuhki , Ohtsuka Masahisa , Miyoshi Norikatsu , Uemura Mamoru , Tanaka Shinji , Calin George Adrian , Mori Masaki , Doki Yuichiro , Eguchi Hidetoshi , Yamamoto Hirofumi 
Published: December 2022

AbstractBackgroundStudies have shown that cancer stemness and the endoplasmic reticulum (ER) stress response are inversely regulated in colorectal cancer (CRC), but the mechanism has not been fully clarified. Long noncoding RNAs (lncRNAs) play key roles in cancer progression and metastasis. In this study we investigated lncRNA 01534 (LINC01534) as a possible modulator between cancer stemness and ER stress response.MethodsIn vitro experiments using CRC cell lines were performed to explore a possible role of LINC01534. The expression of LINC01534 in clinical CRC samples was assessed by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) and in situ hybridization.ResultsSilencing LINC01534 led to suppression of cell proliferation, invasiveness, and cell cycle progression at the G2‐M phase, and promoted apoptosis. Moreover, we found that silencing LINC01534 suppressed cancer stemness, while it activated the ER stress response, especially through the PERK/eIF2α signaling pathway. In situ hybridization revealed LINC01534 was expressed in tumor cells and upregulated in CRC tissues compared with normal epithelium. A survival survey indicated that high LINC01534 expression was significantly associated with shorter overall survival in 187 CRC patients.ConclusionThis is the first report on LINC01534 in human cancer. Our findings suggest that LINC01534 may be an important modulator of the maintenance of cancer stemness and suppression of the ER stress response, and that it could be a novel prognostic factor in CRC.
A novel deleterious ETFA promoter variant causative of multiple acyl‐CoA dehydrogenase deficiency
Authors: Prasun Pankaj , Evans Anthony , Cork Emalyn , Houten Sander M. , Webb Bryn D. 
Published: December 2022

AbstractMultiple acyl‐CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder of fatty acid, amino acid, and choline metabolism. We describe a patient identified through newborn screening in which the diagnosis of MADD was confirmed based on metabolic profiling, but clinical molecular sequencing of ETFA, ETFB, and ETFDH was normal. In order to identify the genetic etiology of MADD, we performed whole genome sequencing and identified a novel homozygous promoter variant in ETFA (c.‐85G > A). Subsequent studies showed decreased ETFA protein expression in lymphoblasts. A promoter luciferase assay confirmed decreased activity of the mutant promoter. In both assays, the variant displayed considerable residual activity, therefore we speculate that our patient may have a late onset form of MADD (Type III). Our findings may be helpful in establishing a molecular diagnosis in other MADD patients with a characteristic biochemical profile but apparently normal molecular studies.
Assembly of Dihydropyridazines via [4+2] Cycloaddition of In Situ Generated Azoalkenes
Authors: Yuan Cheng , Wang Jiatong , Wang Gangqiang , Sun Shaofa , Wang Jian 
Published: December 2022

AbstractThe unprecedented example for the synthesis of multi‐substituted pyridazines via [4+2] cycloaddition of azoalkenes generated in situ is described. This protocol features broad substrate scope and allows rapid assembly of pyridazines. Notably, this process provides an alternative method for in situ generation of azoalkenes.
Highly Bright Near‐Infrared Chemiluminescent Probes for Cancer Imaging and Laparotomy
Authors: Wei Xin , Huang Jingsheng , Zhang Chi , Xu Cheng , Pu Kanyi , Zhang Yan 
Published: December 2022

AbstractNear‐infrared (NIR) chemiluminescence imaging holds potential for sensitive imaging of cancer due to its low background; however, few NIR chemiluminophores are available, which share the drawback of low chemiluminescence quantum yields (ΦCL). Herein, we report the synthesis of NIR chemiluminophores for cancer imaging and laparotomy. Molecular engineering of the electron‐withdrawing group at the para‐position of the phenol‐dioxetane leads to a highly bright NIR chemiluminophore (DPT), showing the ΦCL(4.6×10−2 Einstein mol−1) that is 3 to 5‐fold higher than existing NIR chemiluminophores. By caging the phenol group of DPT with a cathepsin B (CatB) responsive moiety, an activatable chemiluminescence probe (DPTCB) is developed for real‐time turn‐on detection of deeply buried tumor tissues in living mice. Due to its high brightness, DPTCBpermits accurate chemiluminescence‐guided laparotomy.
A Triply Negatively Charged Nanographene Bilayer with Spin Frustration
Authors: Wang Wenqing , Ma Xiao‐Hui , Liu Min , Tang Shuxuan , Ding Xuguang , Zhao Yue , Tan Yuan‐Zhi , Kertesz Miklos , Wang Xinping 
Published: December 2022

AbstractWe report on the largest open‐shell graphenic bilayer and also the first example of triply negatively charged radical π‐dimer. Upon three‐electron reduction, bilayer nanographene fragment molecule (C96H24Ar6)2(Ar=2,6‐dimethylphenyl) (12) was transformed to a triply negatively charged species123⋅−, which has been characterized by single‐crystal X‐ray diffraction, electron paramagnetic resonance (EPR) spectroscopy and magnetic properties on a superconducting quantum interference device (SQUID).123⋅−features a 96‐center‐3‐electron (96c/3e) pancake bond with a doublet ground state, which can be thermally excited to a quartet state. It consists of 34 π‐fused rings with 96 conjugatedsp2carbon atoms. Spin frustration is observed with the frustration parameterf>31.8 at low temperatures in123⋅−, which indicates graphene upon reduction doping may behave as a quantum spin liquid.
Emergence of Visible‐Light Water Oxidation Upon Hexaniobate‐Ligand Entrapment of Quantum‐Confined Copper‐Oxide Cores
Authors: Kumar Tiwari Chandan , Roy Shubasis , Tubul‐Sterin Tal , Baranov Mark , Leffler Nitai , Li Mu , Yin Panchao , Neyman Alevtina , Weinstock Ira A. 
Published: December 2022

AbstractThe formation of small 1 to 3 nm organic‐ligand free metal‐oxide nanocrystals (NCs) is essential to utilization of their attractive size‐dependent properties in electronic devices and catalysis. We now report that hexaniobate cluster‐anions, [Nb6O19]8−, can arrest the growth of metal‐oxide NCs and stabilize them as water‐soluble complexes. This is exemplified by formation of hexaniobate‐complexed 2.4‐nm monoclinic‐phase CuO NCs (1), whose ca. 350 Cu‐atom cores feature quantum‐confinement effects that impart an unprecedented ability to catalyze visible‐light water oxidation with no added photosensitizers or applied potentials, and at rates exceeding those of hematite NCs. The findings point to polyoxoniobate‐ligand entrapment as a potentially general method for harnessing the size‐dependent properties of very small semiconductor NCs as the cores of versatile, entirely‐inorganic complexes.
Modified Smith predictor‐based optimal fractional PD‐PD controller for double integrating processes with considerable time delay
Authors: Sengupta Sayani , Karan Somak , Dey Chanchal 
Published: December 2022

AbstractModified Smith predictor (MSP)‐based fractional propotional derivative (PD)‐PD controller (FO [PD‐PD]) is presented here for controlling double integrating plus time delay (DIPTD) processes. The proposed scheme comprises of two fractional PD controllers together and a first‐order filter to ascertain satisfactory servo as well as regulatory responses. Here, forward path fractional PD controller works towards attaining improved servo response and feedback path fractional PD controller ensures enhanced load regulation. Tuning parameters of both the controllers are obtained through optimization algorithm. The first‐order filter present in the feedback path is responsible for providing improved robustness and is designed with single tuning parameter—closed‐loop time constant (
). Efficacy of the proposed MSP‐based fractional order PD‐PD controller or MSP‐FO (PD‐PD) is substantiated through simulation study, and its superiority is established in comparison with others' recent works on DIPTD processes.
Prediction of Erosive Disease Development by Antimitochondrial Antibodies in Rheumatoid Arthritis
Authors: Moore Richard E. , Wang Ting , Duvvuri Bhargavi , Feser Marie L. , Deane Kevin D. , Solomon Joshua J. , Nelson J. Lee , Demoruelle M. Kristen , Lood Christian 
Published: December 2022

ObjectiveMitochondria are found in the extracellular space in rheumatoid arthritis (RA). However, whether mitochondria are a source of autoantigens in RA has not been carefully addressed. Thus, we undertook this study to investigate the presence and significance of antimitochondrial antibodies (AMAs) in patients with RA.MethodsAMAs were measured in serum samples from 3 cross‐sectional cohorts of RA patients (n = 95, n = 192, and n = 117) and healthy individuals (n = 38, n = 72, and n = 50) using a flow cytometry–based assay. Further, AMAs were detected using an anti–mitofusin‐1 (anti–MFN‐1) IgG enzyme‐linked immunosorbent assay and Western blot analysis. A longitudinal inception cohort, followed up for a median of 8 years, was used to study disease progression.ResultsAMA levels were elevated in RA patients from all 3 cohorts as compared to healthy individuals (P < 0.001, P < 0.05, and P < 0.01), with a range of 14–26% positivity. Levels of anti–MFN‐1 antibodies correlated with AMA levels (r = 0.31, P = 0.006) and were elevated in RA patients as compared to healthy individuals (P < 0.001). The presence of AMAs was associated with erosive disease (P < 0.05) and interstitial lung disease (P < 0.01). Further, AMA levels were found to predict erosive disease (odds ratio [OR] 4.59, P = 0.006) and joint space narrowing (OR 3.08, P = 0.02) independent of anti–citrullinated protein antibodies. Finally, anti–MFN‐1 antibodies identified seronegative patients developing erosive disease (OR 9.33; P = 0.02).ConclusionOur findings demonstrate the presence of novel autoantibodies targeting mitochondria in the setting of RA. AMAs were used to stratify patients based on disease phenotype and to predict development of erosive disease, including in patients with seronegative disease. Our results highlight the essential role of mitochondria in the pathogenesis of RA and suggest a possible benefit of therapies targeting mitochondrial‐mediated inflammation and clearance in these patients.
Insight of smart biosensors for COVID‐19: A review
Authors: Tomichan Rosemary , Sharma Avinash , Akash K. , Siddiqui Adeeb Ahmad , Dubey Amit , Upadhyay Tarun Kumar , Kumar Deepak , Pandey Sadanand , Nagraik Rupak 
Published: December 2022

AbstractThe review discusses the diagnostic application of biosensors as point‐of‐care devices in the COVID‐19 pandemic. Biosensors are important analytical tools that can be used for the robust and effective detection of infectious diseases in real‐time. In this current scenario, the utilization of smart, efficient biosensors for COVID‐19 detection is increasing and we have included a few smart biosensors such as smart and intelligent based biosensors, plasmonic biosensors, field effect transistor (FET) biosensors, smart optical biosensors, surface enhanced Raman scattering (SERS) biosensor, screen printed electrode (SPE)‐based biosensor, molecular imprinted polymer (MIP)‐based biosensor, MXene‐based biosensor and metal–organic frame smart sensor. Their significance as well as the benefits and drawbacks of each kind of smart sensor are mentioned in depth. Furthermore, we have compiled a list of various biosensors which have been developed across the globe for COVID‐19 and have shown promise as commercial detection devices. Significant challenges in the development of effective diagnostic methods are discussed and recommendations have been made for better diagnostic outcomes to manage the ongoing pandemic effectively.
Identifying cathode and anode polarizations during practical high‐rate charging/discharging in different Li‐ion pouch batteries
Authors: Wang Li , Xie Leqiong , Song Youzhi , Liu Xiaomei , Zhang Hao , He Xiangming 
Published: December 2022

AbstractCell design is effective to improve the performances of lithium‐ion batteries (LIBs). For identifying the bottleneck of a full battery used for high‐rate charging/discharging, we developed a simple method, by a reference electrode in practical pouch cells, to quick obtain the polarizations of the cathode and the anode. For a Li(Ni0.6Co0.2Mn0.2)O2/graphite full cell, 63.9% and 97.0% of the polarizations originate from the anode at 50% state of charge (SOC) during 2.0 C charging and discharging rates, respectively. While for LiFePO4/graphite system, 62.5% and 55.8% of the polarizations originate from the anode at the same charging and discharging conditions. These indicate that the anode is the limitation during fast charging/discharging, which is consistent with the common understanding but in contrary to the results obtained by coin cells reported previously. While the rate limitation from anode in LiFePO4/graphite system during fast charging/discharging is significantly changed to both cathode and anode compared with Li(Ni0.6Co0.2Mn0.2)O2/graphite. Besides, graphite anodes in LiFePO4/graphite cells more readily dive to the Li‐metal plating potential at high charging rate. This leads to safety concerns of LiFePO4/graphite cells during fast charging. This is a facile strategy for fast distinguishing polarizations from cathodes and anodes of high‐rate LIBs.
Electrolyte engineering strategies for regulation of the Zn metal anode in aqueous Zn‐ion batteries
Authors: Li Zixuan , Robertson Alex W. 
Published: December 2022

AbstractRechargeable aqueous zinc‐ion batteries (AZBs), with their high theoretical capacity, low cost, safety, and environmental friendliness, have risen as a promising candidate for next‐generation energy storage. Despite the fruitful progress in cathode material research, the electrochemical performance of the AZB remains hindered by the physical and chemical instability of the Zn anode. The Zn anode suffers from dendrite growth and chemical reactions with the electrolyte, leading to efficiency decay and capacity loss. Recently, significant effort has been dedicated to regulating the Zn anode. Electrolyte manipulation, including tailoring the salt, additives, or concentration, is a useful strategy as the electrolyte strongly influences the anode's failure processes. It is thus worthwhile to gain an in‐depth understanding of these electrolyte‐dependent regulation mechanisms. With this in mind, this review first outlines the two main issues behind Zn anode failure, dendrite growth, and side reactions. Subsequently, an understanding of the electrolyte tailoring strategy, namely, the influence of the salt, additive, and concentration on the Zn anode, is provided. We conclude by summarizing the future prospects of the Zn metal anode and potential electrolyte‐based solutions.