bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Study on Anti-oxidant and Anti-cancer Perspectives of Rutin
Authors: Tiwari Prashant , Mishra Rakhi , Mazumder Rupa , Mazumder Avijit , Singh Ayushi 
Published: May 2023

Introduction:
The purpose of this study was to extract, isolate, and evaluate in vitro the antioxidant
and anticancer properties of rutin from Citrus reticulata and Citrus limon.

Background:
Bioflavonoids are a class of polyphenolic secondary metabolites that give plants their
distinctive organoleptic properties. Rutin, also known as rutoside, is a citrus flavonoid that is found in a
variety of plants, including citrus fruits, and has a wide range of pharmacological effects.

Objective:
This investigation's goal was to extract, isolate, and test the antioxidant and anti-cancer
properties of rutin in vitro.

Methods:
By incorporating the appropriate solvent system, the Soxhlet apparatus was used to extract C.
reticulata (R2) and C. limon (R3) from dried peel powder. Column chromatography was used in the
isolation process. Various solvent systems on the basis of their polarity were used to isolate rutin,
which further was evaluated for anti-oxidant and anti-cancer action by the use of in vitro tests.

Results:
Successful extraction and isolation of rutin from two sources were achieved. Additionally,
their antioxidant and anticancer activity was also evaluated by in vitro methods. Both isolated rutins
(R2 and R3) possessed optimal antioxidants in the range of 31.64 to 76.28 g/ml and anticancer activity
with IC50 values of 4 to 7 g/ml, when compared to standard doxorubicin with an IC50 value of 3g/ml. In
the comparison of rutin isolated from C. reticulata with rutin isolated from C. limon, rutin isolated from
C. reticulata was found to be more abundant and more potent in terms of yield and activity.

Conclusion:
The study's findings are clear; Citrus reticulata species have a higher flavonoid and phenolic
content, and the rutin extracted from them is an effective anti-oxidant and anti-cancer agent
against lung cancer. The study can be used as a model for future studies on rutin’s role in cancer.
Inferring the Functional Effect of Gene-body H3K79me2 Signals in Normal
Samples on Gene Expression Changes: A Potential Susceptibility
Marker in Chronic Myelogenous Leukemia
Authors: Zhang Lu-Qiang , Hao Yu-Duo , La Ta , Li Qian-Zhong 
Published: May 2023

Background:
Current identification of chronic myelogenous leukemia markers tends to mine
diagnostic or prognostic biomarkers, ignoring susceptibility markers in normal samples.

Objective:
We aim to identify possible susceptibility markers for preventing chronic myelogenous leukemia.

Methods:
Functional links of H3K79me2 patterns and gene expression changes were inferred by correlation
analyses. DNase-seq read distribution, transcription factor motifs, and their binding data were acquired
via ceasBW and HOMER. Normalized transcription factor binding signals were submitted to a
random forest algorithm to predict susceptibility gene expression changes. Three strategies were performed
to validate the influence of low H3K79me2 signals on gene expression changes.

Results:
The gene-body H3K79me2 signals in normal samples were negatively related to gene expression
changes during leukemogenesis (ρ=-0.92), regardless of gene lengths and expression levels. Characterization
revealed that genes with lower H3K79me2 signals in normal samples have more open environments.
Transcription factors GATA3, GATA4, TEAD1, TEAD3, TEAD4, and TRPS1 may induce
the upregulation of up-susceptibility genes (ρ=0.95), and ASCL2, IRF4, IRF3, E2A, OCT4, and ZEB2
may mediate the downregulation of down-susceptibility genes (ρ=0.97). Enrichment analysis implied
that the screened susceptibility genes were involved in leukemia-related pathways, and about 50% of
leukemia stem cell differentially expressed genes were included in these genes. Besides, all hub genes
extracted from susceptibility genes were well documented in different leukemia subtypes. Finally, the
effect of H3K79me2 signals on gene expression changes were validated in a mouse model and three cell
models.

Conclusion:
Low gene-body H3K79me2 signals in normal samples may serve as susceptibility markers
for chronic myelogenous leukemia.
Factors Predicting Older People’s Acceptance of a Personalized Health Care Service App and the Effect of Chronic Disease: Cross-Sectional Questionnaire Study
Authors: Koo Jun Hyuk , Park You Hyun , Kang Dae Ryong 
Published: May 2023

Background
Mobile health (mHealth) services enable real-time measurement of information on individuals’ biosignals and environmental risk factors; accordingly, research on health management using mHealth is being actively conducted.

Objective
The study aims to identify the predictors of older people’s intention to use mHealth in South Korea and verify whether chronic disease moderates the effect of the identified predictors on behavioral intentions.

Methods
A cross-sectional questionnaire study was conducted among 500 participants aged 60 to 75 years. The research hypotheses were tested using structural equation modeling, and indirect effects were verified through bootstrapping. Bootstrapping was performed 10,000 times, and the significance of the indirect effects was confirmed through the bias-corrected percentile method.

Results
Of 477 participants, 278 (58.3%) had at least 1 chronic disease. Performance expectancy (β=.453; P=.003) and social influence (β=.693; P<.001) were significant predictors of behavioral intention. Bootstrapping results showed that facilitating conditions (β=.325; P=.006; 95% CI 0.115-0.759) were found to have a significant indirect effect on behavioral intention. Multigroup structural equation modeling testing the presence or absence of chronic disease revealed a significant difference in the path of device trust to performance expectancy (critical ratio=–2.165). Bootstrapping also confirmed that device trust (β=.122; P=.039; 95% CI 0.007-0.346) had a significant indirect effect on behavioral intention in people with chronic disease.

Conclusions
This study, which explored the predictors of the intention to use mHealth through a web-based survey of older people, suggests similar results to those of other studies that applied the unified theory of acceptance and use of technology model to the acceptance of mHealth. Performance expectancy, social influence, and facilitating conditions were revealed as predictors of accepting mHealth. In addition, trust in a wearable device for measuring biosignals was investigated as an additional predictor in people with chronic disease. This suggests that different strategies are needed, depending on the characteristics of users.
Impact of Multimedia Messaging Service Education and Exercise Social Support on Physical Activity Among Patients With Type 2 Diabetes: Quasi-Experimental Study
Authors: Alyahya Mohammad S , Al-Sheyab Nihaya A , Khader Yousef S , Alqudah Jumana A 
Published: May 2023

Background
Social support is one of the interpersonal stimuli that define an individual’s predisposition to engage in health-promoting behaviors and is considered a facilitator in improving health habits. Patients with type 2 diabetes mellitus (T2DM) can benefit from educating supportive families and friends on self-care management including exercise behavior. Multimedia messaging service (MMS) could also be an effective method for delivering targeted educational interventions that focus on physical activity (PA).

Objective
This study aimed to assess the effectiveness of MMS educational interventions and perceived social support for exercise on level of PA of patients with T2DM.

Methods
A quasi-experimental pretest-posttest design was conducted to recruit 98 patients with T2DM. The intervention group received MMS education aiming to improve exercise social support and PA level for 2 months, and their counterparts in the control group received the usual routine care. We sent 2 to 3 messages daily for 2 weeks from Saturday to Thursday (12 days total). These messages were a combination of videos and texts, and the evidence-based content of these messages was reviewed and approved by the advisory committee. We randomly assigned eligible patients in a 1:1 ratio into the intervention or the control groups. Participants completed a survey in 3 periods.

Results
There were no significant differences in friends’ support, family verbal, practical, or emotional support over time in the intervention group (P>.05). Yet, there was a small effect size (Cohen d) in friends’ social support (0.389), family practical support (0.271), and moderate activities (0.386). A medium effect size was found in family verbal (0.463) and emotional (0.468) support. Being married increased the likelihood of friends’ support by 2.3 times after intervention (P=.04), whereas rarely doing exercise decreased the likelihood of friends’ support by 28% (P=.03) and family practical support by 28% (P=.01). Being female and married increased the likelihood of doing moderate activities by 1.6 times (P=.002) and 1.5 times (P=.049) in the intervention group. Being a housewife decreased the likelihood of doing moderate activities by 20% (P=.001). Finally, being a female with a higher educational level decreased the likelihood of doing hard activities by 20% (P=.04) and 15% (P=.002), respectively.

Conclusions
A theoretically based MMS health education targeting PA levels and social support of family and friends to perform PA seems promising in promoting family and friends’ social support and improving PA levels among patients with T2DM. Actively involving family and friends in educational interventions that target PA can have an impact on health-promoting behaviors in patients with diabetes.
Participant Engagement in Microrandomized Trials of mHealth Interventions: Scoping Review
Authors: Leong Utek , Chakraborty Bibhas 
Published: May 2023

Background
Microrandomized trials (MRTs) have emerged as the gold standard for the development and evaluation of multicomponent, adaptive mobile health (mHealth) interventions. However, not much is known about the state of participant engagement measurement in MRTs of mHealth interventions.

Objective
In this scoping review, we aimed to quantify the proportion of existing or planned MRTs of mHealth interventions to date that have assessed (or have planned to assess) engagement. In addition, for the trials that have explicitly assessed (or have planned to assess) engagement, we aimed to investigate how engagement has been operationalized and to identify the factors that have been studied as determinants of engagement in MRTs of mHealth interventions.

Methods
We conducted a broad search for MRTs of mHealth interventions in 5 databases and manually searched preprint servers and trial registries. Study characteristics of each included evidence source were extracted. We coded and categorized these data to identify how engagement has been operationalized and which determinants, moderators, and covariates have been assessed in existing MRTs.

Results
Our database and manual search yielded 22 eligible evidence sources. Most of these studies (14/22, 64%) were designed to evaluate the effects of intervention components. The median sample size of the included MRTs was 110.5. At least 1 explicit measure of engagement was included in 91% (20/22) of the included MRTs. We found that objective measures such as system usage data (16/20, 80%) and sensor data (7/20, 35%) are the most common methods of measuring engagement. All studies included at least 1 measure of the physical facet of engagement, but the affective and cognitive facets of engagement have largely been neglected (only measured by 1 study each). Most studies measured engagement with the mHealth intervention (Little e) and not with the health behavior of interest (Big E). Only 6 (30%) of the 20 studies that measured engagement assessed the determinants of engagement in MRTs of mHealth interventions; notification-related variables were the most common determinants of engagement assessed (4/6, 67% studies). Of the 6 studies, 3 (50%) examined the moderators of participant engagement—2 studies investigated time-related moderators exclusively, and 1 study planned to investigate a comprehensive set of physiological and psychosocial moderators in addition to time-related moderators.

Conclusions
Although the measurement of participant engagement in MRTs of mHealth interventions is prevalent, there is a need for future trials to diversify the measurement of engagement. There is also a need for researchers to address the lack of attention to how engagement is determined and moderated. We hope that by mapping the state of engagement measurement in existing MRTs of mHealth interventions, this review will encourage researchers to pay more attention to these issues when planning for engagement measurement in future trials.
KARAGENIN SEBAGAI MODEL INFLAMASI PADA KULIT MENCIT: GAMBARAN MAKROSKOPIK DAN HISTOPATOLOGIS
Authors: Widyarini Sitarina , Sugiyono Sugiyono , Kristianingrum Yuli Purwandari , Sutrisno Bambang 
Published: May 2023

Penelitian ini bertujuan untuk membuat model inflamasi kulit pada punggung mencit betina galur Swiss dengan menggunakan karagenin dengan berbagai konsentrasi. Pada penelitian ini akan digunakan 45 ekor mencit betina galur Swiss umur 2 bulan yang dibagi dalam tiga kelompok secara acak. Kelompok I diberi karagenin 1%. Kelompok II diberi karagenin 2%, dan kelompok III diberi karagenin 4%. Induksi inflamasi dengan karagenin dilakukan melalui injeksi sub kutan karagenin dengan volume 0,1 mL pada kulit punggung mencit. Sebelum dilakukan injeksi karagenin tebal lipat kulit (skin-fold thickness) diukur dengan menggunakan jangka sorong digital. Selanjutnya setiap jam setelah injeksi sampai dengan jam ke- 6 pasca injeksi. Hewan coba selanjutnya dietanasi dengan cara dislokasi servikalis pada akhir jam ke-6. Organ kulit diambil dengan memotong kulit pada area dimana injeksi karagenin dilakukan dan selanjutnya diawetkan dengan formalin 10% untuk pemeriksaan histopatologis dengan pewarnaan hematoxylin eosin. Analisis data tebal lipat kulit dianalisis dengan Two Ways Anova dan diikuti dengan post hoc test Tukey test. Hasil penelitian memperlihatkan ada perbedaan bermakna pada tebal lipat kulit antar kelompok perlakuan (p<0,05). Injeksi subkutaneuos karagenin 1%, 2% dan 4% meningkatkan tebal lipat kulit sebesar 35%, 50% dan 60% pada jam pertama pasca injeksi (p<0,05). Inflamasi mild dan moderate pada bagian dermis ditemukan pasca injeksi karagenin 1% dan 2%. Infamasi severe pada bagian dermis dan panikulus ditemukan pasca injeksi karagenin 4%. Karagenin dapat dipakai sebagai model inflamasi akut pada kulit punggung mencit.
Analisis Pemberian Serbuk Jahe Merah, Kunyit, dan Temulawak Dengan Metode Insilico dan Invivo pada Ayam Broiler
Authors: . Herawati , Al Jabbar Renanda Nur , Oktanella Yudit 
Published: May 2023

This study aimed to eveluate effect of combination powder of red ginger (Zingiber officinale var. Rubrum), turmeric (Curcuma domestica), and temulawak (Curcuma xanthorrhiza) on the calculation of normal Liberkhun crypts by insilico and invivo methods. Molecular docking conducted in the pocket site of Interferon Lambda Receptor (IFN-λR) to modulate anti-inflammatory activity in chicken caecum. The study used 20 day old chickens (DOC) which were randomly grouped into 5 groups: negative control without treatment (P0) and powder addition group {0,5% (P1); 1%(P2); 1.5%(P3); 2%(P4)}. All chickens were reared for 35 days then chickens were euthanized using halal slaughter method and caecum organs were collected for histopathological observations. all active substances red ginger (gingerol, shogaol); turmeric (curcumin,tetrahydrocurcumin); and temulawak (demethoxycurcumin, bisdemethoxycurcumin) can bind to IFN-λR. Curcumin (-6.0 kcal/mol) have highest affinity values. Invivo treatment showed significant effect (p<0.05) on normal calculation of Liberkhun crypts due to tissue damage. Tukey's test confirmed that negative group was significantly different from P2, P3, and P4 with an average (4.35 ± 1.09b; 5.6 ± 1.54c; and 5.7 ± 1.78c). This research suggests that the addition of combination powder by 1,5% can potentially used for anti-inflamatory agent that confirmed by insilico method.
Evaluation of the Clinical Efficacy of Prednisolone in the Treatment of Inflammatory Bowel Diseases with Different Dosage Methods
Authors: Alekseeva O. P. , Krishtopenko S. V. , Alekseeva A. A. 
Published: May 2023

Aim: to investigate the clinical efficacy of two methods of oral dosing of prednisolone (in mg and mg/kg) for the induction of remission for patients with ulcerative colitis (UC) and Crohn's disease (CD) using the technology of constructing and evaluating the effectiveness function (dose-effect relationship).Material and methods. In this study were included 86 patients aged from 18 to 65 years with moderate or severe active inflammatory bowel disease (61 — UC, 25 — CD). All patients were treated with prednisolone at an initial daily dose from 30 to 60 mg with a subsequent tapering of dose. The clinical response to treatment was evaluated at the time of complete withdrawal of prednisolone using the generally accepted criteria. Two efficiency functions were constructed, compared and analyzed: the first — at the initial dosage of prednisolone in mg and the second calculating the dose in mg/kg of patient weight. The patients' body weight ranged from 41 to 98 kg. The “dose-effect” relationship for prednisolone was constructed with statistical transformation of the baseline clinical data and a quantitative expression of the actual doses and alternative responses into a graph of the effectiveness function. The mean value at each point was estimated based on the regression kernel scoring method.Results. Two graphs of the “dose-effect” of prednisolone in mg and mg/kg of patient weight were constructed. The optimal clinically effective dose (OCED) when calculated in mg/kg of weight was 0.70 ± 0.01 (0.68 + 0.72) mg/kg with the corresponding effect 79.25 ± 6.26 (66.62 91.88) %. When two graphs in mg and mg / kg of weight were superimposed, it is shown that when an initial dose of 40 mg is prescribed without taking into account the patient's weight, the effect of therapy will be 25 % lower. Prescribing a dose of 60 mg per day without weight will be optimal for patients with a body weight of 85-90 kg. With a lower body weight, the clinical effect will not decrease, but the likelihood of recognized side effects of prednisolone should be expected in proportion to the decrease in body weight.Conclusion. The clinical efficacy of two methods of prednisolone dosing (mg and mg/kg) for patients with IBD during the first induction course was compared.Using a new technology for constructing and evaluating the effectiveness function (dose-effect relationship) allowed us to prove a reliable relationship between the body weight of patients with the clinical effect of prednisolone in patients with UC and CD. Based on the analysis of the dose-effect relationship, the optimal clinically effective dose of prednisolone for patients with UC and CD during the first induction course was established, equal to 0.70 mg/kg, which can be recommended for use in clinical practice for calculating individual doses.
Diagnostic Possibilities of Determining the Level of Faecal Calprotectin in Clinical Practice
Authors: Sheptulin A. A. , Kardasheva S. S. , Kurbatova A. A. 
Published: May 2023

Aim: to analyze the publications devoted to the modern possibilities of determining the level of faecal calprotectin (FCP) in the diagnosis of various diseases.Key points. In patients with already established diagnosis of inflammatory bowel diseases (IBD), dynamic monitoring of the level of FCP allows to assess the course and prognosis of the disease, as well as the effectiveness of treatment. The determining of FCP helps in the primary diagnosis of IBD (ulcerative colitis, Crohn's disease, microscopic colitis), contributing to their differentiation from functional bowel disorders, as well as in assessing the course of diverticular intestinal disease and celiac disease. The possibility of using FCP as a marker of colorectal cancer (CRC) and adenomatous polyps of the colon is also discussed.Conclusion. Determining the level of FCP plays an important role in the diagnosis and assessment of the course of a number of gastroenterological diseases (primarily IBD). The significance of FCP as a marker of CRC requires further research.
Modern Approaches to <i>H. pylori</i> Eradication Therapy in Adults (Literature Review and Resolution of Experts Council)
Authors: Ivashkin V. Т. , Ulyanin A. I. , Mayev I. V. , Kozlov R. S. , Livzan M. A. , Abdulkhakov S. R. , Alekseyeva O. P. , Alekseyenko S. A. , Bordin D. S. , Dekhnich N. N. , Korochyanskaya N. V. , Lapina T. L. , Poluektova E. A. , Simanenkov V. I. , Trukhmanov A. S. , Khlynov I. B. , Tsukanov V. V. , Sheptulin A. A. 
Published: May 2023

Aim: to analyze current approaches to H. pylori eradication therapy in adults and present the materials of Experts Council held on December 9, 2022 in Moscow.General statements. H. pylori infection is the main etiological factor of gastritis, peptic ulcer, and gastric cancer. Eradication of H. pylori is recognized as a necessary measure to reduce the incidence of these diseases. The approaches to selecting an eradication regimen should be optimized to take into account epidemiological trends and achieve better treatment outcomes. The updated Maastricht VI Consensus Report presents the means to overcome the difficulties in selecting an approach to the treatment of H. pylori infection. However, eradication therapy remains challenging due to adverse events (primarily antibiotic-associated diarrhea), poor treatment tolerance and patient compliance. Eradication therapy can be optimized by supplementing treatment regimens with strain-specific probiotics that reduce adverse events, improve patient compliance and eradication rates, such as Saccharomyces boulardii CNCM I-745 strain with established efficacy.Conclusion. The inclusion of certain probiotics in eradication regimens improves treatment tolerance, reduces the risk of adverse events, improves patient compliance and eradication rates.
Protective Renalase Deficiency in β-Cells Shapes Immune Metabolism and Function in Autoimmune Diabetes
Authors: Bode Kevin , MacDonald Tara , Stewart Taylor , Mendez Bryhan , Cai Erica P. , Morrow Noelle , Lee Yu-Chi , Yi Peng , Kissler Stephan 
Published: May 2023

Type 1 diabetes (T1D) is caused by the immune-mediated loss of pancreatic β-cells that produce insulin. The latest advances in stem cell (SC) β-cell differentiation methods have made a cell replacement therapy for T1D feasible. However, recurring autoimmunity would rapidly destroy transplanted SC β-cells. A promising strategy to overcome immune rejection is to genetically engineer SC β-cells. We previously identified Renalase (Rnls) as a novel target for β-cell protection. Here we show that Rnls deletion endows β-cells with the capacity to modulate the metabolism and function of immune cells within the local graft microenvironment. We used flow cytometry and single-cell RNA sequencing to characterize β-cell graft-infiltrating immune cells in a mouse model for T1D. Loss of Rnls within transplanted β-cells affected both the composition and the transcriptional profile of infiltrating immune cells in favor of an anti-inflammatory profile with decreased antigen-presenting capacity. We propose that changes in β-cell metabolism mediate local immune regulation and that this feature could be exploited for therapeutic goals.

Article Highlights
Protective Renalase (Rnls) deficiency impacts β-cell metabolism. Rnls-deficient β-cell grafts do not exclude immune infiltration. Rnls deficiency in transplanted β-cells broadly modifies local immune function. Immune cell in Rnls mutant β-cell grafts adopt a noninflammatory phenotype.