bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic heat stress induces renal fibrosis and mitochondrial dysfunction in laying hens
Authors: Nanto-Hara Fumika , Yamazaki Makoto , Murakami Hitoshi , Ohtsu Haruhiko 
Published: June 2023

AbstractBackgroundHeat stress in laying hens negatively affects egg production and shell quality by disrupting the homeostasis of plasma calcium and phosphorus levels. Although the kidney plays an important role in calcium and phosphorus homeostasis, evidence regarding the effect of heat stress on renal injury in laying hens is yet to be elucidated. Therefore, the aim of this study was to evaluate the effects of chronic heat stress on renal damage in hens during laying periods.MethodsA total of 16 white-leghorn laying hens (32 weeks old) were randomly assigned to two groups (n = 8). One group was exposed to chronic heat stress (33 °C for 4 weeks), whereas the other group was maintained at 24 °C.ResultsChronic heat exposure significantly increased plasma creatinine and decreased plasma albumin levels (P < 0.05). Heat exposure also increased renal fibrosis and the transcription levels of fibrosis-related genes (COLA1A1,αSMA, andTGF-β) in the kidney. These results suggest that renal failure and fibrosis were induced by chronic heat exposure in laying hens. In addition, chronic heat exposure decreased ATP levels and mitochondrial DNA copy number (mtDNA-CN) in renal tissue, suggesting that renal mitochondrial dysfunction occurs under conditions of heat stress. Damaged mitochondria leak mtDNAs into the cytosol and mtDNA leakage may activate the cyclic GMP-AMP synthase (cGAS) stimulator of interferon genes (STING) signaling pathway. Our results showed that chronic heat exposure activated the cGAS-STING pathway as indicated by increased expression ofMDA5,STING,IRF7,MAVS, andNF-κBlevels. Furthermore, the expression of pro-inflammatory cytokines (IL-12) and chemokines (CCL4andCCL20) was upregulated in heat-stressed hens.ConclusionsThese results suggest that chronic heat exposure induces renal fibrosis and mitochondrial damage in laying hens. Mitochondrial damage by heat stress may activate the mtDNA-cGAS-STING signaling and cause subsequent inflammation, which contributes to the progression of renal fibrosis and dysfunction.
Review of deep learning methods for remote sensing satellite images classification: experimental survey and comparative analysis
Authors: Adegun Adekanmi Adeyinka , Viriri Serestina , Tapamo Jules-Raymond 
Published: June 2023

AbstractClassification and analysis of high-resolution satellite images using conventional techniques have been limited. This is due to the complex characteristics of the imagery. These images are characterized by features such as spectral signatures, complex texture and shape, spatial relationships and temporal changes. In this research, we present the performance evaluation and analysis of deep learning approaches based on Convolutional Neural Networks and vision transformer towards achieving efficient classification of remote sensing satellite images. The CNN-based models explored include ResNet, DenseNet, EfficientNet, VGG and InceptionV3. The models were evaluated on three publicly available EuroSAT, UCMerced-LandUse and NWPU-RESISC45 datasets containing categories of images. The models achieve promising results in accuracy, recall, precision and F1-score. This performance demonstrates the feasibility of Deep Learning approaches in learning the complex and in-homogeneous features of the high-resolution remote sensing images.
Reversible data hiding based on histogram and prediction error for sharing secret data
Authors: Islamy Chaidir Chalaf , Ahmad Tohari , Ijtihadie Royyana Muslim 
Published: June 2023

AbstractWith the advancement of communication technology, a large number of data are constantly transmitted through the internet for various purposes, which are prone to be illegally accessed by third parties. Therefore, securing such data is crucial to protect the transmitted information from falling into the wrong hands. Among data protection schemes, Secret Image Sharing is one of the most popular methods. It protects critical messages or data by embedding them in an image and sharing it with some users. Furthermore, it combines the security concepts in that private data are embedded into a cover image and then secured using the secret-sharing method. Despite its advantages, this method may produce noise, making the resulting stego file much different from its cover. Moreover, the size of private data that can be embedded is limited. This research works on these problems by utilizing prediction-error expansion and histogram-based approaches to embed the data. To recover the cover image, the SS method based on the Chinese remainder theorem is used. The experimental results indicate that this proposed method performs better than similar methods in several cover images and scenarios.
Development and validation of ICPMS methods for simultaneous determination of elemental impurities in topical cream containing ximenynic acid
Authors: Shivatare Rakesh , Jangra Sarita , Gaikwad Asmita , Kewatkar Shailesh , Bhutale Neetin , Suryavanshi Dhanaji S. , Tare Harshal 
Published: June 2023

Abstract
Background
These days, the presence of simple impurities in pharmaceuticals is a major cause for worry. This is because some contaminants are dangerous on their own, and even small impurities can make a drug less stable and shorten its shelf life. The goal of this study was to see if creams with ximenynic acid could be tested with inductively coupled plasma-mass spectrometry to find out how much arsenic, mercury, lead, cadmium, vanadium, cobalt, and nickel were in them. The best way to do things would be one that was quick, accurate, sensitive, and very productive (ICP-MS). The method included both inductively coupled plasma mass spectrometry (ICP-MS) and microwave digestion.

Results
Seven of the seven linearity correlation coefficient (‘R’) value were more than 0.99. LOD values were calculated using 33% of the 0.25 J threshold. Six LOQ responses (0.25 J level) were taken after considerable discussion. Calculated and reported %RSD for six LOQ copies. All elemental impurities Vanadium (V), Cobalt (Co), Nickel (Ni), Arsenic (As), Cadmium (Cd), Mercury (Hg), and Lead (Pb) were recovered between 83.33% and 115.97%, within acceptability limits. RSD% for procedure precision and intermediate precision data never exceeded 5%. The available evidence shows that the ICP-MS technique is a good way to measure these components.

Conclusion
The statistical analysis showed that the developed ICP-MS method for measuring elements in Topical Cream with Ximenynic Acid is selective and accurate. Since this ICPMS method is good at estimating several elements simultaneously, it could be used to check for elemental contaminants in the formulation.
Cytological and Ultrasound Features of Thyroid Nodules Correlate With Histotypes and Variants of Thyroid Carcinoma
Authors: Sgrò Daniele , Brancatella Alessandro , Greco Giuseppe , Torregrossa Liborio , Piaggi Paolo , Viola Nicola , Rago Teresa , Basolo Fulvio , Giannini Riccardo , Materazzi Gabriele , Elisei Rossella , Santini Ferruccio , Latrofa Francesco 
Published: June 2023

Abstract

Context
Prognosis is excellent for papillary thyroid carcinoma (PTC), noninvasive follicular thyroid neoplasia with papillary-like nuclear features (NIFT-P), and follicular thyroid carcinoma (FTC) but is poor for poorly differentiated thyroid carcinoma (PDTC) and anaplastic thyroid carcinoma (ATC). Among PTCs, the prognosis is more favorable for follicular (FV-PTC) and classic (CV-PTC) than for tall cell (TCV-PTC), and solid (SV-PTC) variants.

Objective
To associate histotypes and variants of thyroid carcinoma with ultrasound and cytological features.

Methods
Histology of 1018 benign tumors and 514 PTC (249 CV, 167 FV, 49 TC, 34 SV, and 15 other variants), 52 NIFT-P, 50 FTC, 11 PDTC, and 3 ATC was correlated with fine-needle aspiration biopsy categories (Italian classification: TIR1, TIR2, TIR3A, TIR3B, TIR4, and TIR5) and ultrasound features at the Endocrinology Unit, University Hospital of Pisa. In total, 1117 patients with thyroid nodule(s) who underwent thyroidectomy were included.

Results
Of PTC, 36.3% had indeterminate cytology (TIR3A or TIR3B), 56.6% were suspicious for malignancy or malignant (TIR4 or TIR5); 84.0% FTC and 69.3% NIFT-P were TIR3A or TIR3B; 72.5% FV-PTC and 73.6% SV-PTC were TIR3A or TIR3B; 79.9% CV-PTC and 95.9% TCV-PTC were TIR4 or TIR5. The association of a hypoechoic pattern, irregular margins, and no microcalcifications was more frequent in TCV-PTC than in CV-PTC (P = .02, positive predictive value = 38.9%; negative predictive value = 85.5%).

Conclusion
At cytology, most FTC, NIFT-P, FV-PTC, and SV-PTC were indeterminate, most CV-PTC and TCV-PTC were suspicious for malignancy or malignant. Ultrasound can be helpful in ruling out TCV-PTC.
Age But Not Menopausal Status Is Linked to Lower Resting Energy Expenditure
Authors: Karppinen Jari E , Wiklund Petri , Ihalainen Johanna K , Juppi Hanna-Kaarina , Isola Ville , Hyvärinen Matti , Ahokas Essi K , Kujala Urho M , Laukkanen Jari , Hulmi Juha J , Ahtiainen Juha P , Cheng Sulin , Laakkonen Eija K 
Published: June 2023

Abstract

Context
It remains uncertain whether aging before late adulthood and menopause are associated with fat-free mass and fat mass–adjusted resting energy expenditure (REEadj).

Objectives
We investigated whether REEadj differs between middle-aged and younger women and between middle-aged women with different menopausal statuses. We repeated the age group comparison between middle-aged mothers and their daughters to partially control for genotype. We also explored whether serum estradiol and FSH concentrations explain REEadj in midlife.

Methods
We divided 120 women, including 16 mother-daughter pairs, into age groups; group I (n = 26) consisted of participants aged 17 to 21, group II (n = 35) of those aged 22 to 38, and group III (n = 59) of those aged 41 to 58 years. The women in group III were further categorized as pre- or perimenopausal (n = 19), postmenopausal (n = 30), or postmenopausal hormone therapy users (n = 10). REE was assessed using indirect calorimetry, body composition using dual-energy X-ray absorptiometry, and hormones using immunoassays.

Results
The REEadj of group I was 126 kcal/day [95% confidence interval (CI): 93-160] higher than that of group III, and the REEadj of group II was 88 kcal/day (95% CI: 49-127) higher. Furthermore, daughters had a 100 kcal/day (95% CI: 63-138 kcal/day) higher REEadj than their middle-aged mothers (all P &lt; .001). In group III, REEadj was not lower in postmenopausal women and did not vary by sex hormone concentrations.

Conclusions
We demonstrated that REEadj declines with age in women before late adulthood, also when controlling partially for genetic background, and that menopause may not contribute to this decline.
A genome-wide genetic screen identifies CYRI-B as a negative regulator of CEACAM3-mediated phagocytosis
Authors: Kuiper Johannes W. P. , Krause Julia , Potgeter Leon , Adrian Jonas , Hauck Christof R. 
Published: June 2023

ABSTRACT
Opsonin-independent phagocytosis mediated by human carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) has evolved to control a subset of human-restricted bacterial pathogens. CEACAM3 engagement triggers rapid GTP-loading of the small GTPase Rac as a master regulator of cytoskeletal rearrangements and lamellipodia-driven internalization. To identify components of the CEACAM3-initiated signaling cascade, we performed a genome-wide CRISPR/Cas9-based screen in human myeloid cells. Following infection with fluorescently labeled bacteria, cells exhibiting elevated phagocytosis (gain-of-function) as well as cells showing reduced phagocytosis (loss-of-function) were sorted and enrichment of individual single-guide RNAs (sgRNAs) was determined by next generation sequencing. Concentrating on genes whose targeting by three distinct sgRNAs consistently resulted in a gain-of-function phenotype, we identified the Rac-GTP-sequestering protein CYRI-B as a negative regulator of CEACAM3-mediated phagocytosis. Clonal HL-60 cell lines with CYRI-B knockout showed enhanced CEACAM3-downstream signaling, such as Rac GTP loading and phosphorylation of PAK kinases, leading to increased phagocytosis of bacteria. Complementation of the CYRI-B knockout cells reverted the knockout phenotype. Our results unravel components of CEACAM3-initiated opsonin-independent phagocytosis on a genome-wide level and highlight CYRI-B as a negative regulator of CEACAM3-initiated signaling in myeloid cells.
Progress for carbon dioxide geological storage in West Macedonia: A field and laboratory-based survey
Authors: Tyrologou Pavlos , Vamvaka Agnes , Koukouzas Nikolaos , Pedro Jorge , Fleury Marc , Carneiro Julio , Ribeiro Carlos , Ghikas Dina , Mpatsi Anna , Barradas João Pedro , Faria Paula , De Mesquita Lobo Veloso Fernanda 
Published: June 2023

Background: It is widely acknowledged that carbon dioxide (CO2), a greenhouse gas, is largely responsible for climatic changes that can lead to warming or cooling in various places. This disturbs natural processes, creating instability and fragility of natural and social ecosystems. To combat climate change, without compromising technology advancements and maintaining production costs at acceptable levels, carbon capture and storage (CCS) technologies can be deployed to advance a non-disruptive energy transition. Capturing CO2 from industrial processes such as thermoelectric power stations, refineries, and cement factories and storing it in geological mediums is becoming a mature technology. Part of the Mesohellenic Basin, situated in Greek territory, is proposed as a potential area for CO2 storage in saline aquifers. This follows work previously done in the StrategyCCUS project, funded by the EU. The work is progressing under the Pilot Strategy, funded by the EU. Methods: The current investigation includes geomechanical and petrophysical methods to characterise sedimentary formations for their potential to hold CO2 underground. Results: Samples were found to have both low porosity and permeability while the corresponding uniaxial strength for the Tsotyli formation was 22 MPa, for Eptechori 35 MPa and Pentalofo 74 MPa. Conclusions: The samples investigated indicate the potential to act as rock caps due to low porosity and permeability, but fluid pressure within the rock should remain within specified limits; otherwise, the rock may easily fracture and result in CO2 leakage or/and deform to allow the flow of CO2. Further investigation is needed to identify reservoir rocks as well more sampling to allow for statistically significant results.
Strong-field photoionization/dissociation of vinyl bromide in near-infrared and ultraviolet femtosecond laser fields
Authors: Liu Yang , He Shen , Zhou Longxing , Chen Qi , Zhao Yiwen , Sun Tian , Shen Xingchen , Yu Tao , Lv Hang , Xu Haifeng 
Published: June 2023

Ionization of polyatomic molecules in strong femtosecond laser fields is a pivotal process that may induce a variety of highly nonlinear molecular physical phenomena. We performed an experimental study on photoionization and dissociation of vinyl bromide molecules in 800 nm or 400 nm femtosecond strong laser fields using a time-of-flight mass spectrometer. The dependence of the ion yields on laser intensity and polarization was investigated, and the results are compared in different wavelength laser fields. The kinetic energy releases from Coulomb explosion of vinyl bromide, and the angular distributions of different fragment ions were obtained. The mechanism of strong-field ionization/dissociation of vinyl bromide in different laser fields is discussed based on the experimental results.
High frequency of Voltage-gated sodium channel (VGSC) gene mutations in Aedes albopictus (Diptera: Culicidae) suggest rapid insecticide resistance evolution in Shanghai, China
Authors: Yuan Hao , Shan Wenqi , Zhang Yuhang , Yan Hanlu , Li Yikai , Zhou Qiuming , Dong Haowei , Tao Feng , Liu Hongxia , Leng Peien , Peng Heng , Ma Yajun 
Published: June 2023

Background
Dengue fever is an infectious disease that is imported into Shanghai, China and requires prevention and control measures. Controlling the vector Aedes albopictus through insecticide use is a key approach to dengue control. However, the rapid evolution of insecticide resistance in Ae. albopictus has raised concerns about the failure of dengue control efforts. Knockdown resistance (kdr) caused by point mutations in the voltage-gated sodium channel (VGSC) gene is a primary mechanism of pyrethroid resistance. In this study, we investigated the kdr mutations of Ae. albopictus in Shanghai and evaluated the trend in its evolution.

Methodology/principal findings
We collected 17 populations of Ae. albopictus from 15 districts in Shanghai in 2020, extracted genomic DNA from individual mosquitoes, and amplified Domain II, III, and IV in VGSC using PCR. Following sequencing, we obtained 658 VGSC sequences. We detected the nonsynonymous mutations V1016G, I1532T, and F1534S/C/I, among which V1016G and F1534C/I were reported in Shanghai for the first time and F1534I was a novel mutant allele in Ae. albopictus. The overall mutation frequency was 84.65%, with individual mutation frequencies ranging from 46.81% to 100%, excluding the Fengxian District population, which had a frequency of 0%. The V1016G and I1532T mutation types accounted for 7.14% and 3.42%, respectively. The mutant allele at codon 1534 accounted for 63.98% of all mutations, including TCC/S (62.77%), TGC/C (1.06%), and ATC/I (0.15%). We identified and classified five intron types in Domain III by length, including A (83 bp, 12.07%), B (68 bp, 87.30%), C (80 bp, 0.16%), D (72 bp, 0.16%), and E (70 bp, 0.31%). Individuals with intron B had a significant mutation tendency at codon 1534 relative to intron A (chi-square test, p < 0.0001). We found no correlation between mutation frequency and the amount of pyrethroid used (Pearson correlation, p = 0.4755).

Conclusions/significance
In recent years, kdr mutations in the Ae. albopictus population in Shanghai have rapidly evolved, as evidenced by an increase in mutation types and significantly increased mutation frequency. The F1534I/ATC mutant allele was found to be a novel mutation, F1534C/TGC was reported for the first time in Shanghai, and intron B in Domain III was significantly associated with mutation frequency at codon 1534. Continuous monitoring of resistance changes and strict regulation of insecticide use are required.
Extraction and generalisation of category-level information during visual statistical learning in autistic people
Authors: Parsons Owen , Baron-Cohen Simon 
Published: June 2023

Background
We examined whether information extracted during a visual statistical learning task could be generalised from specific exemplars to semantically similar ones. We then looked at whether performance in autistic people differed to non-autistic people during a visual statistical learning task and specifically examined whether differences in performance between groups occurred when sequential information was presented at a semantic level. We did this by assessing recall performance using a two-alternative forced choice paradigm after presenting participants with a sequence of naturalistic scene images.

Methods
125 adult participants (61 participants with an autism diagnosis and 64 non-autistic controls) were presented with a fast serial presentation sequence of images and given a cover task to avoid attention being explicitly drawn to patterns in the underlying sequences. This was followed by a two-alternative forced choice task to assess participants’ implicit recall. Participants were presented with 1 of 3 unique versions of the task, in which the presentation and assessment of statistical regularities was done at either a low feature-based level or a high semantic-based level.

Results
Participants were able to generalise statistical information from specific exemplars to semantically similar ones. There was an overall significant reduction in visual statistical learning in the autistic group but we were unable to determine whether group differences occurred specifically in conditions where the learning of semantic information was required.

Conclusions
These results provide evidence that participants are able to extract statistical information that is presented at the level of specific exemplars and generalise it to semantically similar contexts. We also showed a modest but statistically significant reduction in recall performance in the autistic participants relative to the non-autistic participants.
Pregnancy outcomes in women with gestational hypertension and preeclampsia at Paropakar Maternity and Women’s Hospital, Nepal: A retrospective study
Authors: Das Seema , Maharjan Renusha , Bajracharya Rashmita , Shrestha Rabina , Karki Sulata , Das Rupesh , Odland Jon Øyvind , Odland Maria Lisa 
Published: June 2023

Introduction
Gestational hypertension and preeclampsia are the most common types of hypertensive disorder in pregnancy and these conditions are associated with adverse maternal and fetal outcomes. This study aims to determine the differences in pregnancy outcomes in women with gestational hypertension and preeclampsia.

Methods
A retrospective study was done at The Paropakar Maternity and Women’s Hospital, a tertiary level hospital, in the Kathmandu, Nepal. Pregnant women who had given birth at the hospital between September 17 and December 18 of 2017 were included. Data were obtained from the non-digitalized hospital records. The adjusted odds ratio (AOR) and 95% confidence interval were computed using logistic regression analysis. Multivariable analysis of pregnancy outcomes (cesarean sections, low birth weight, and preterm birth) was adjusted for maternal age, parity, twin birth, gestational age, calcium supplementation, and maternal co-morbidity.

Results
Preeclampsia was strongly associated with cesarean section compared to normal pregnancies (OR = 8.11, p<0.001). Whereas the odds of cesarean section among women with gestational hypertension was almost 2 times (OR = 1.89, p<0.001). Preterm birth was not significantly associated with gestational hypertension but was associated with preeclampsia (OR = 3.39, p<0.001). Gestational hypertension and preeclampsia were not associated with low birth weight.

Conclusion
In Nepal, women who develop preeclampsia seem at higher risk of having adverse pregnancy outcomes than women with gestational hypertension. These findings should be considered by national health authorities and other health organizations when setting new priorities to improve pregnancy outcomes.