bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of Neutrophil Activation by Phosphodiesterase 4 Blockade in Behçet's Disease
Authors: Le Joncour Alexandre , Régnier Paul , Maciejewski‐Duval Anna , Charles Erwan , Barete Stéphane , Fouret Pierre , Rosenzwajg Michelle , Klatzmann David , Cacoub Patrice , Saadoun David 
Published: March 2023

ObjectiveBehçet's disease (BD) is a systemic vasculitis with inflammatory lesions mediated by cytotoxic T cells and neutrophils. Apremilast, an orally available small‐molecule drug that selectively inhibits phosphodiesterase 4 (PDE4), has been recently approved for the treatment of BD. We aimed to investigate the effect of PDE4 inhibition on neutrophil activation in BD.MethodsWe studied surface markers and reactive oxygen species (ROS) production by flow cytometry, and neutrophil extracellular traps (NETs) production and molecular signature of neutrophils by transcriptome analysis before and after PDE4 inhibition.ResultsActivation surface markers (CD64, CD66b, CD11b, and CD11c), ROS production, and NETosis were up‐regulated in BD patient neutrophils compared to healthy donor neutrophils. Transcriptome analysis revealed 1,021 significantly dysregulated neutrophil genes between BD patients and healthy donors. Among dysregulated genes, we found a substantial enrichment for pathways linked to innate immunity, intracellular signaling, and chemotaxis in BD. Skin lesions of BD patients showed increased infiltration of neutrophils that colocalized with PDE4. Inhibition of PDE4 by apremilast strongly inhibited neutrophil surface activation markers as well as ROS production, NETosis, and genes and pathways related to innate immunity, intracellular signaling, and chemotaxis.ConclusionWe highlight key biologic effects of apremilast on neutrophils in BD.
Anti–Neutrophil Extracellular Trap Antibodies in Antiphospholipid Antibody–Positive Patients: Results From the Antiphospholipid Syndrome Alliance for Clinical Trials and InternatiOnal Networking Clinical Database and Repository
Authors: Zuo Yu , Navaz Sherwin , Tsodikov Alex , Kmetova Katarina , Kluge Lyndsay , Ambati Amala , Hoy Claire K. , Yalavarthi Srilakshmi , de Andrade Danieli , Tektonidou Maria G. , Sciascia Savino , Pengo Vittorio , Ruiz‐Irastorza Guillermo , Belmont H. Michael , Gerosa Maria , Fortin Paul R. , de Jesus Guilherme Ramires , Branch D. Ware , Andreoli Laura , Rodriguez‐Almaraz Esther , Petri Michelle , Cervera Ricard , Willis Rohan , Karp David R. , Li Quan‐Zhen , Cohen Hannah , Bertolaccini Maria Laura , Erkan Doruk , Knight Jason S. ,   the Antiphospholipid Syndrome Alliance for Clinical Trials and InternatiOnal Networking
Published: March 2023

ObjectiveThis study aimed to elucidate the presence, antigen specificities, and potential clinical associations of anti–neutrophil extracellular trap (anti‐NET) antibodies in a multinational cohort of antiphospholipid (aPL) antibody–positive patients who did not have lupus.MethodsAnti‐NET IgG/IgM levels were measured in serum samples from 389 aPL‐positive patients; 308 patients met the classification criteria for antiphospholipid syndrome. Multivariate logistic regression with best variable model selection was used to determine clinical associations. For a subset of the patients (n = 214), we profiled autoantibodies using an autoantigen microarray platform.ResultsWe found elevated levels of anti‐NET IgG and/or IgM in 45% of the aPL‐positive patients. High anti‐NET antibody levels are associated with more circulating myeloperoxidase (MPO)–DNA complexes, which are a biomarker of NETs. When considering clinical manifestations, positive anti‐NET IgG was associated with lesions affecting the white matter of the brain, even after adjusting for demographic variables and aPL profiles. Anti‐NET IgM tracked with complement consumption after controlling for aPL profiles; furthermore, patient serum samples containing high levels of anti‐NET IgM efficiently deposited complement C3d on NETs. As determined by autoantigen microarray, positive testing for anti‐NET IgG was significantly associated with several autoantibodies, including those recognizing citrullinated histones, heparan sulfate proteoglycan, laminin, MPO–DNA complexes, and nucleosomes. Anti‐NET IgM positivity was associated with autoantibodies targeting single‐stranded DNA, double‐stranded DNA, and proliferating cell nuclear antigen.ConclusionThese data reveal high levels of anti‐NET antibodies in 45% of aPL‐positive patients, where they potentially activate the complement cascade. While anti‐NET IgM may especially recognize DNA in NETs, anti‐NET IgG species appear to be more likely to target NET‐associated protein antigens.
From invitation to destination: A systematic literature review of the use of picturebooks in inquiry‐based education
Authors: Oberman Rowan 
Published: March 2023

AbstractPicturebooks offer powerful supports for teaching diverse content across different curriculum areas. Research and curriculum resources document their use in inquiry‐based education practices, particularly in relation to science and philosophy. There is, however, little theoretical analysis of this use or cross‐curricular comparisons. Applying a systematic literature review process, this paper examines existing empirical research on the use of picturebooks in formal inquiry‐based education contexts. The analysis considers the context in which the picturebooks were used, including curriculum context and class level, the role played by the picturebooks in the inquiries and the value ascribed to the use of picturebooks. The review proposes a framework for understanding and contrasting the varying roles played by picturebooks in inquiry‐based education approaches. This framework involves seven uses: invitations, provocations, mentors, models, resources, touchstones and destinations. Varying benefits are attributed to the use of picturebooks, including their practicality, inclusivity, complexity, aid to cognitive learning, emotional engagement and association with child readers. These benefits emphasise both the accessibility and the complexity of picturebooks. The review proposes that inquiry approaches should attend to picturebook form and content and the interdependency of the two. The review's analysis supports extended use of picturebooks across the curriculum, including in history, geography, citizenship and science education, as well as in interdisciplinary approaches.
Cell factory engineering: Challenges and opportunities for synthetic biology applications
Authors: Bachhav Bhagyashree , de Rossi Jacopo , Llanos Carlos D. , Segatori Laura 
Published: March 2023

AbstractThe production of high‐quality recombinant proteins is critical to maintaining a continuous supply of biopharmaceuticals, such as therapeutic antibodies. Engineering mammalian cell factories presents a number of limitations typically associated with the proteotoxic stress induced upon aberrant accumulation of off‐pathway protein folding intermediates, which eventually culminate in the induction of apoptosis. In this review, we will discuss advances in cell engineering and their applications at different hierarchical levels of control of the expression of recombinant proteins, from transcription and translational to posttranslational modifications and subcellular trafficking. We also highlight challenges and unique opportunities to apply modern synthetic biology tools to the design of programmable cell factories for improved biomanufacturing of therapeutic proteins.
pH‐Responsive and Reversible A‐Motif‐Based DNA Hydrogel: Synthesis and Biosensing Application**
Authors: Morya Vinod , Shukla Ashish Kumar , Ghoroi Chinmay , Bhatia Dhiraj 
Published: March 2023

AbstractFunctional DNA hydrogels with various motifs and functional groups require perfect sequence design to avoid cross‐bonding interference with themselves or other structural sequences. This work reports an A‐motif functional DNA hydrogel that does not require any sequence design. A‐motif DNA is a noncanonical parallel DNA duplex structure containing homopolymeric deoxyadenosines (poly‐dA) strands that undergo conformation changes from single strands at neutral pH to a parallel duplex DNA helix at acidic pH. Despite this and other advantages over other DNA motifs like no cross‐bonding interference with other structural sequences, the A‐motif has not been explored much. We successfully synthesized a DNA hydrogel by using an A‐motif as a reversible handle to polymerize a DNA three‐way junction. The A‐motif hydrogel was initially characterized by electrophoretic mobility shift assay, and dynamic light scattering, which showed the formation of higher‐order structures. Further, we used imaging techniques like atomic force microscopy and scanning electron microscope to validating its hydrogel like highly branched morphology. pH‐induced conformation transformation from monomers to gel is quick and reversible, and was analysed for multiple acid‐base cycles. The sol‐to‐gel transitions and gelation properties were further examined in rheological studies. The use of the A‐motif hydrogel in the visual detection of pathogenic target nucleic acid sequence was demonstrated for the first time in a capillary assay. Moreover, pH‐induced hydrogel formation was observed in situ as a layer over the mammalian cells. The proposed A‐motif DNA scaffold has enormous potential in designing stimuli‐responsive nanostructures that can be used for many biological applications.
Transvenous retrograde thoracic duct embolization for effective treatment of recurrent plastic bronchitis after fontan palliation
Authors: Haddad Raymond N. , Dautry Raphael , Bonnet Damien , Malekzadeh‐Milani Sophie 
Published: March 2023

AbstractWe report the case of a 5.5‐year‐old patient (16 kg/105 cm) who presented with plastic bronchitis (PB) refractory to conservative treatment 3 months after completion of Fontan palliation. Bi‐inguinal transnodal fluoroscopy‐guided lymphangiogram confirmed the chylous leak originating from the thoracic duct (TD) into the chest and did not opacify any central lymphatic vessel for direct transabdominal puncture. Retrograde transfemoral approach was adopted to catheterize the TD and selectively embolize its caudal portion using microcoils and liquid embolic adhesive. Recurrence of symptoms after 2 months indicated a redo catheterization to occlude the TD entirely using the same technique. The procedure was successful and the patient was discharged after 2 days with sustained clinical improvement at 24 months postoperative. In the context of refractory PB, end‐to‐end transvenous retrograde embolization of the TD appears to be an interesting alternative to more complex interventions such as transabdominal puncture, decompression, or surgical ligation of the TD.
Successful treatment with bortezomib for POEMS syndrome, overcoming complicated severe heart block
Authors: Nakamura Yuichi , Itoh Yoshihiro , Wakimoto Naoki , Kanno Ryu , Iida Shinichirou , Yamamoto Keiji 
Published: March 2023

AbstractCardiac bradyarrhythmia and conduction disorder may be rare, but recurrent adverse events caused by bortezomib. Here we report a case with POEMS syndrome presenting severe heart block after bortezomib plus dexamethasone therapy. After permanent pacemaker implantation, bortezomib was restarted and maintained, resulting in sustained complete response for POEMS syndrome.
Experimental Investigations of Heat Pipe Systems for Use in Electrical Engines
Authors: Margraf Hendrik , Lottis Marian , Deeb Mohammad , Luke Andrea 
Published: March 2023

AbstractMit dem Ziel die Effizienz elektrischer Arbeitskraftmaschinen durch ein innovatives Kühlsystem zu erhöhen, wird ein Wärmetransportsystem auf Basis von Wärmerohren entwickelt und analysiert. Die Leistungsfähigkeit des Systems bei Variation des Temperaturbereichs und des zugeführten Wärmestroms sowie der Einfluss der Rotation werden experimentell untersucht. Der Wärmewiderstand des Systems wird im Vergleich zu reiner Wärmeleitung um 98 % reduziert. Die Rotation des Systems hat keinen signifikanten Einfluss auf den Wärmewiderstand, der bis zum Erreichen der Leistungsgrenze konstant ist. Die Leistungsgrenze ist temperaturabhängig und stimmt annähernd mit der Kapillarkraftgrenze überein.
Untersuchung überlagerter Effekte von Druckverlust und Totvolumen durch Rekuperation am isobaren Expansionsmotor
Authors: Mügge Nils , Kronberg Alexander , Glushenkov Maxim , Kenig Eugeny Y. 
Published: March 2023

AbstractDie Wirtschaftlichkeit von Wärmemotoren wird maßgeblich durch die Rückführung von Wärme in Rekuperatoren beeinflusst. Beim isobaren Expansionsmotor vom Bush‐Typ, einem Wärmemotor, der bereits mit Wärmequellen ab 60 °C betrieben werden kann, steigt der thermische Wirkungsgrad durch den Einsatz von Rekuperatoren signifikant. Gleichzeitig wirken sich das Fluidvolumen des Wärmeübertragers sowie der beim Betrieb auftretende Druckverlust negativ auf den thermischen Wirkungsrad aus. In dieser Arbeit wird der Zusammenhang von Totvolumen, Druckverlust und Wärmerückgewinnung untersucht und Empfehlungen für den Betrieb von Rekuperatoren am isobaren Expansionsmotor gegeben.
Phototheranostic Nanoagents for Imaging‐Guided Treatment of Oral Cancer†
Authors: Li Jisen , Zhang Yufan , Qu Jiafei , Gao Jing , Ding Dan , Shen Jing 
Published: March 2023

Comprehensive SummaryPhototherapy showed almost a lack of drug resistance and, depending on the therapeutic effects of non‐invasive light‐stimulating photosensors. The side effects of phototherapy were greatly reduced compared to their traditional equivalents. Phototheranostic nanoagents had new properties in drug delivery, biocompatibility, targeting and response, in which traditional phototheranostic drugs can not possess. Recently, a large number of relevant studies have demonstrated that photodynamic therapy (PDT) in combination with other agents and image‐guided multifunctional photothermal therapy (PTT) were well suited for the treatment of oral cancer. Through the design of the nanoagents, researchers have discovered various applications for phototherapy, such as targeted release of co‐packaged drugs, multifunctional imaging for diagnosis and treatment combination, accurate targeting caused by nanocarriers, and synergistic chemotherapy with phototherapy. In this paper, we first reviewed the research related to phototheranostic nanoagents for image‐guided treatment of oral cancer. We tried to introduce the design concept and the treatment effect by three parts of components of phototheranostic nanoagents, categories of phototheranostic nanoagents and application of phototheranostic nanoagents. It also provided a reference for nanomaterial development and clinical applications in research of oral cancer treatment.
Importance of the Nature of Acidic Sites of Different Aluminosilicates on Fixation of Carbon Dioxide with Styrene Oxide
Authors: Ramesh Aishwarya , Ray Saumi 
Published: March 2023

AbstractThe nature of acidic sites in commercially available aluminosilicates, zeolite Na−Y, zeolite NH4+‐ZSM‐5, and as‐synthesized Al‐MCM‐41 have been investigated by employing them as catalysts for capturing CO2 by styrene oxide. The catalysts, in tandem with tetrabutylammonium bromide (TBAB), produce styrene carbonate, and the yield of the product is observed to be governed by the acidity of the catalysts and hence, the Si/Al ratio. All these aluminosilicate frameworks have been characterized by IR, BET, TGA, and XRD. XPS, NH3‐TPD, and 29Si solid‐state NMR studies have been carried out to analyze the Si/Al ratio and the acidity of these catalysts. According to TPD studies, the number of weak acidic sites of these materials follow an order as NH4+‐ZSM‐5 < Al‐MCM‐41 < zeolite Na−Y, which is just in accordance with their Si/Al ratios and the yield of the cyclic carbonates obtained, i. e., 55.3 %, 68 %, and 75.4 % respectively. The TPD data and the yield of the product carried out with calcined zeolite Na−Y indicate that not only the weak acidic sites but also the role of strong acidic sites might appear crucial in the cycloaddition reaction.
Bernoulli polynomial‐based control technique for PV‐integrated grid system under distorted supply
Authors: Rai Kanchan Bala , Singh Alka , Kumar Narendra 
Published: March 2023

SummaryThis paper discusses the performance of the Bernoulli Polynomial‐based artificial neural network (BePANN) for the control of a single‐phase grid‐integrated solar photovoltaic (PV) system. A single‐phase, single‐stage topology of a grid‐integrated PV system is utilized to feed nonlinear loads at the point of common coupling. The fundamental load component is extracted from distorted load current using a single‐layer neural network. There are several control techniques mentioned in the literature for load compensation. However, many techniques show slower convergence response, higher oscillations, and a large computational burden. The designed BePANN controller attenuates harmonic components from nonlinear current and improves the power quality (PQ) under normal and distorted grid conditions. The single‐layer BePANN control has a simplified structure based on several polynomial terms; this reduces the computational burden and complexity of the controller. The objective of the designed controller is to fulfill the load's active power demand from the generated solar PV power and feed the excess power back to the grid when surplus. When solar PV is not integrated with the grid, the voltage source converter acts as a distribution static compensator, improving the system's utilization factor. The proposed control technique is simulated in MATLAB Simulink, and results are experimentally verified through laboratory prototype under normal and abnormal grid conditions. The application of higher order polynomials for PV‐integrated systems with multifunctional PQ capabilities has been implemented for the first time in this article. The main outcomes of the proposed control technique are solving current‐related PQ issues and reactive power compensation and providing improved power factor under both normal and distorted polluted grids.
Triaxial creep damage characteristics of sandstone under high crustal stress and its constitutive model for engineering application
Authors: Chen Dongxu , Wang Laigui , Versaillot Pierre Darry , Sun Chuang 
Published: March 2023

AbstractThe creep characteristics of rock under high crustal stress are of important influence on the long‐term stability of deep rock engineering. To study the creep characteristics and engineering application of sandstone under high crustal stress, this study constructed nonlinear creep damage (NCD) constitutive mode based on the triaxial graded loading‒unloading creep test of sandstone in the Yuezhishan Tunnel. A numerical NCD constitutive model and a breakable lining (BL) model were developed based on FLAC3D and then applied to the stability analysis of the Yuezhishan Tunnel. Based on the creep test results of sandstone, a power function of creep rate and stress level was constructed, by which the long‐term strength was solved. The results show that the long‐term strength of the red sandstone based on the related function of the steady‐state creep rate and stress level is close to the measured stress value in engineering. The NCD model considering damage factors reflects the instantaneous and viscoelastic plasticity deformation characteristics of the red sandstone. The numerical NCD constitutive model and the BL model can reflect surrounding rock deformation characteristics and lining failure characteristics in practical engineering. The research results provide theoretical references for long‐term stability analysis of rock engineering and the deformation control of surrounding rock under high crustal stress.
Enhanced Performance of Transfer‐Free Graphene Transparent Conductive Films on Insulating Substrates by Introducing Array Nanostructure
Authors: Lu Meng , Zhang Xiaoling , Yin Wanying , Wang Yao , Li Dezeng , Zhao Qingbiao 
Published: March 2023

AbstractGraphene is one of the most prevailing materials to replace traditional transparent conductive films (TCFs). In order to improve the performance of graphene TCFs, it is proposed to directly deposit graphene on insulating substrates via assisted catalysis of Cu foil to avoid intricate transfer process, and further effectively enhance the optical performance by introducing the close‐packed antireflection array nanostructure. The graphene TCFs grown on bare quartz substrates have sheet resistance of 0.766 kΩ sq−1 and transmittance of 86.83 %. Graphene composite TCFs with transmittance of 95.79 % were obtained by introducing the close‐packed solid/hollow SiO2 nanospheres antireflection array nanostructure. Compared with graphene TCFs, transmittance of graphene composite TCFs increased significantly, while the sheet resistance remained at the same level. This method combining direct synthesis technique with novel antireflection array nanostructure provides a significant design idea to grow transfer‐free graphene with controllable optoelectronic properties and promotes the application and development of the future electronic devices.
Pyridine Adducts of Tricyano‐ and Dicyanoboranes
Authors: Riefer Jarno , Zapf Ludwig , Wirthensohn Raphael , Hennig Philipp T. , Ribbeck Tatjana , Sprenger Jan A. P. , Ignat'ev Nikolai V. , Finze Maik 
Published: March 2023

AbstractCyanoborane adducts of the Lewis acids B(CN)3, BF(CN)2, and BH(CN)2 with pyridine and 4‐cyanopyridine have been obtained in high yields. The syntheses were accomplished by oxidation of the readily available potassium salts of the cyano(hydrido)borate anions [BH(CN)3]− (MHB), [BFH(CN)2]− (FHB), and [BH2(CN)2]− (DHB) with bromine in the presence of the respective pyridine derivative C5H5N or 4‐CN‐C5H4N as starting material. All six cyanoborane adducts have been characterized by NMR and vibrational spectroscopy, elemental analysis, and single‐crystal X‐ray diffraction. The reduction of the cyanoborane adducts has been investigated by cyclic voltammetry and the Lewis acidity of the different cyanoboranes has been assessed using the Gutmann‐Beckett method. Selected experimental data and trends are compared to theoretical ones, for example fluoride ion affinities (FIAs).
Carbon black supported PtCu nanoparticles derived from mix‐phased Cu precursor for high‐performanced oxygen reduction reaction in acid medium
Authors: Xie Peng , Wang Yingqian , Yao Pengfei , Zhang Dezheng , Zhang Huimin , Cao Jing , Liu Cong , Mei Xuanhao , Song Ping , Gong Xue , Han Ce , Xu Weilin 
Published: March 2023

AbstractAlloying high‐cost Pt with transition metals has been considered as an effective route to synthesize the electrocatalysts with low Pt loading and excellent activity towards oxygen reduction reaction (ORR) under acid solution. The galvanic replacement method, as featured with efficiency and simplicity, is widely reported to produce Pt‐based bimetallic alloys and thereby declare the significance of reductive transition metal precursor on the enhancement of ORR performance. Herein, mix‐phased Cu−Cu2O precursor was applied to prepare carbon black supported highly dispersed PtCu alloy nanoparticles (PtCu/C). The proper Cu−Cu2O ratios can exactly facilitate the generation of small sized PtCu alloy nanoparticles with regulated bimetallic content. Meanwhile, the Cu2O phase is revealed to benefit the electron transfer from Pt to Cu and thus improve the intrinsic activity of Pt active sites. And the metallic Cu can favor the promotion of electrochemical active surface area. Consequently, the as‐prepared PtCu/C behaves impressive ORR activity with half‐wave potential of 0.88 V (vs. RHE) and mass activity of 0.49 A cm−2 mgPt−1 at 0.8 V, which is 9.8 times of commercial Pt/C catalysts. Our work will offer helpful advices for the development and regulation of novel Pt‐based alloy materials towards diverse electrocatalysis.