bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simplified and Highly-reliable automated production of [18F]FSPG for clinical studies
Authors: Lin Mai , Ta Robert T. , Manning H. Charles 
Published: June 2023

Abstract
Background
(S)-4-(3-18F-Fluoropropyl)-L-Glutamic Acid ([18F]FSPG) is a positron emission tomography (PET) tracer that specifically targets the cystine/glutamate antiporter (xc-), which is frequently overexpressed in cancer and several neurological disorders. Pilot studies examining the dosimetry and biodistribution of ([18F]FSPG in healthy volunteers and tumor detection in patients with non-small cell lung cancer, hepatocellular carcinoma, and brain tumors showed promising results. In particular, low background uptake in the brain, lung, liver, and bowel was observed that further leads to excellent imaging contrasts of [18F]FSPG PET. However, reliable production-scale cGMP-compliant automated procedures for [18F]FSPG production are still lacking to further increase the utility and clinical adoption of this radiotracer. Herein, we report the optimized automated approaches to produce [18F]FSPG through two commercially available radiosynthesizers capable of supporting centralized and large-scale production for clinical use.
Results
Starting with activity levels of 60–85 GBq, the fully-automated process to produce [18F]FSPG took less than 45 minutes with average radiochemical yields of 22.56 ± 0.97% and 30.82 ± 1.60% (non-decay corrected) using TRACERlab™ FXFN and FASTlab™, respectively. The radiochemical purities were > 95% and the formulated [18F]FSPG solution was determined to be sterile and colorless with the pH of 6.5–7.5. No radiolysis of the product was observed up to 8 hours after final batch formulation.
Conclusions
In summary, cGMP-compliant radiosyntheses and quality control of [18F]FSPG have been established on two commercially available synthesizers leveraging high activity concentration and radiochemical purity. While the clinical trials using [18F]FSPG PET are currently underway, the automated approaches reported herein will accelerate the clinical adoption of this radiotracer and warrant centralized and large-scale production of [18F]FSPG.
Lessons learned from the experiences and perspectives of frontline healthcare workers on the COVID-19 response
Authors: Orhierhor Marian , Pringle Wendy , Halperin Donna , Parsons Janet , Halperin Scott A. , Bettinger Julie A. 
Published: June 2023

Abstract
Background
During the COVID-19 pandemic, healthcare systems and healthcare workers (HCWs) faced significant demands and unique challenges. In this qualitative study, we explore the effects of the COVID-19 public health policies on British Columbia’s frontline HCWs, describe what worked in the management of the pandemic, and elucidate the lessons learned that could be applied to future pandemic preparedness, recovery and response.
Methods
This qualitative descriptive study is part of a larger, national multi-case study on pandemic policy communication and uptake. Semi-structured interviews were conducted from November 2020- June 2021 with fourteen HCWs working in long-term care (LTC), acute care and public health settings. Data were inductively coded, and analyzed following a resilience framework for public health emergency preparedness, which emphasizes the essential elements of a public health system, vital to all phases of health emergency management, readiness, response and recovery.
Results
HCWs experienced confusion, frustration, uncertainty, anxiety, fatigue and stress, during the pandemic and detailed challenges that affected policy implementation. This included communication and coordination inconsistencies between the province and regional health authorities; lack of involvement of frontline staff in pandemic planning; inadequate training and support; inadequate personal protective equipment resource capacity and mobilization; and staffing shortages. HCWs recommended increased collaboration between frontline staff and policy makers, investment in preparing and practicing pandemic plans, and the need for training in emergency management and infection prevention and control.
Conclusions
Pandemic planning, response and recovery should include inputs from actors/key stakeholders at the provincial, regional and local levels, to facilitate better coordination, communication and outcomes. Also, given the critical roles of frontline HCWs in policy implementation, they should be adequately supported and consideration must be given to how they interpret and act on policies. Bi-directional communication channels should be incorporated between policymakers and frontline HCWs to verify the appropriate adoption of policies, reflective learning, and to ensure policy limitations are being communicated and acted upon by policy makers.
Biomimetic anisotropic macroporous cryogel promotes the repair of osteoporotic bone defects through altering the ROS niche via down-regulating the ROMO1
Authors: Liu Hai , Pan Weilun , Liu Honglin , Xie Denghui , Liao Liqiong 
Published: June 2023

Abstract
Background Osteoporosis is a systemic bone disease prone to fractures due to decreased bone density and bone quality. The pathological environment of osteoporosis interferes with the normal process of fracture healing. The clinical regenerative repair materials specifically used for osteoporotic bone defects are not satisfactory, and the study of the mechanism of bone defect regeneration in the pathological environment of osteoporosis is helpful for the design of related materials.Methods In this study, the cryogels were prepared from decellularized extracellular matrix (dECM), methacrylate gelatin (GelMA), and carboxymethyl chitosan (CMCS) via unidirectional freezing, photocrosslinking, and genipin crosslinking. dECM extracted from normal or osteoporotic rats was applied for the preparation of the cryogels, named as GelMA-CMCS@Normal or GelMA-CMCS@OVX, respectively. The effects of different cryogels on BMSCs isolated from osteoporotic rats (OVX-BMSCs) were observed in vitro and in vivo.Results It was verified that the cryogels had excellent in vitro and in vivo biocompatibility. Furthermore, the GelMA-CMCS@Normal could effectively improve the proliferation of OVX-BMSCs, and promote the differentiation of OVX-BMSCs into osteoblasts in vitro and in vivo. RNA sequencing found that the OVX-BMSCs co-cultured with GelMA-CMCS@Normal cryogel exhibited down-regulated expression of reactive oxygen species modulator 1 (Romo1), which could activate the expression of nuclear factor erythroid 2-related factor 2 (Nfe2l2, NRF2). Further evidence showed that the reactive oxygen species (ROS) of the OVX-BMSCs were scavenged effectively after co-cultured with the GelMA-CMCS@Normal cryogel.Conclusions The results indicated that GelMA-CMCS@Normal cryogel was expected to be a clinical candidate for the repair of osteoporotic bone defects by regulating the ROS niche of OVX-BMSCs.Trial registration: Not applicable.
A prognostic model of clear cell renal cell carcinoma based on telomere-related lncRNAs
Authors: Chen Hao , Li Li , Mao Longkun , Zeng Jianfeng 
Published: June 2023

Abstract
Background
Telomeres have been demonstrated to be critical in the development of multiple tumors. However, the association of telomere-related lncRNAs with clear cell renal cell carcinoma (ccRCC) and their prognostic roles in ccRCC patients remain unknown.
METHODS
Expression matrix and clinicopathological data of ccRCC patients were extracted from The Cancer Genome Altas and UCSC Xena browser. The differentially expressed genes were identified and intersected with the telomere-related genes downloaded from the Telnet database. Telomere-related lncRNAs were screened by the univariate Cox regression analysis. Each patient's risk score was calculated to establish a nomogram based on eight telomere-related lncRNAs screened by the least absolute shrinkage and selection operator (LASSO) algorithm and multivariate Cox regression analysis. The correlation between telomere-related lncRNAs and immune cells was assessed by the CIBEERSORT algorithm. The immune and stromal infiltrations were quantified by the ESTIMATE algorithm. Gene set enrichment analysis (GSEA) was performed to explore the selected lncRNA functions.
Result
We screened eight telomere-related lncRNAs and established a risk score model for predicting survival in ccRCC patients. A nomogram was developed to predict the survival outcomes of postoperative patients by integrating several clinical factors, and a well-predictive effect was observed. The correlation between selected lncRNAs and immune function was explored by the CIBEERSORT and ESTIMATE algorithms. Besides, GSEA showed that telomere-related lncRNAs could affect ccRCC prognosis through multiple pathways.
Tensile strength of polyester fiber estimated by molecular-chain extension prior to structure formation
Authors: Ohkoshi Yutaka , Tomisawa Ren , Nagata Mutsuya , Otsuka Yumu , Ikaga Toshifumi , Kim KyoungHou , Okada Kazuyuki , Kanaya Toshiji , Katsuta Hiroo 
Published: June 2023

Abstract
The combination of laser irradiation heating and synchrotron X-ray sources has made it possible to observe the fiber-structure development that occurs at sub-millisecond timescales after necking during continuous drawing. Through wide-angle X-ray diffraction (WAXD) and small-angle X-ray scattering (SAXS) analysis of poly(ethylene terephthalate) fibers of three different molecular weights drawn under equivalent stresses, a good correlation was observed between the d-spacing of smectic (001') diffraction extrapolated to the necking point and the strength of the drawn fiber. This indicates that the molecular chains that bear the drawing stress also bear most of the applied stress during tensile testing of the resultant fiber. In addition, considering the drawing-stress dependence of the d-spacing and the molecular weight distribution of the fiber revealed that molecular chains with molecular weights over 23,000 g/mol bear the majority of tensile force applied to the fiber.
FOXO1：An Indicator for Prognosis of Lung Adenocarcinoma and Squamous Carcinoma from a Comprehensive Analysis of Bioinformatics
Authors: Zhang Hui , Zong Ming , Cheng Kai , Fu Yan , Yu Xueying , Man Qiuhong 
Published: June 2023

Abstract
Background
Patients diagnosed with lung adenocarcinoma and lung squamous carcinoma typically face a poor clinical prognosis. There is an urgent need to identify biomarkers that can enhance the potential clinical prognoses and guide lung cancer treatment. FOXO1, a tumour suppressor, is frequently inactivated in various human cancers and may be correlated with patients' prognosis.
Methods
In this study, we utilised comprehensive online databases, including Oncomine, Kaplan-Meierplotter, and PrognoScan, to investigate the expression of FOXO1 and its relationship with patients' prognosis. To generate informative plots, we employed sophisticated analytical tools such as R-language and the Xiantao academic platform.
Results
Our findings revealed downregulation of FOXO1 expression in both lung adenocarcinoma and lung squamous carcinoma, which exhibited a significant positive correlation with overall survival and recurrence-free survival. Notably, in lung adenocarcinoma, the anti-tumour activity of FOXO1 appeared to be predominantly associated with central memory T cells, mast cells, T helper cells, and helper T cells 2. Conversely, in lung squamous carcinoma, the anti-tumour effect seemed to be related to central memory T cells, mast cells, macrophages, and gamma delta T cells.
Conclusions
Based on our results, FOXO1 shows potential as a prognostic marker for predicting the survival outcomes of patients with lung adenocarcinoma and lung squamous carcinoma. Further validation and exploration of FOXO1's role in these cancer types are warranted to advance our understanding and potentially improve clinical management strategies.
Clinical features and prognostic factors of acute glufosinate ammonium
Authors: Huang Changbao , Xie Donghui , Shen Yulan , Xue Xiang 
Published: June 2023

Abstract
This retrospective study was conducted on 67 patients who suffered from acute oral glufosinate ammonium poisoning to summarize a comprehensive overview of the clinical characteristics. General clinical data and laboratory test results were collected from the time of admission to hospital discharge. The most common clinical symptom of GA poisoning were nausea and vomiting, The incidence of unfavorable outcomes and the mortality of GA poisoning were 41.8% and 13.4%. The unfavorable outcomes group had a higher initial serum ammonia level (75.7±17.3umol/L vs 47.5±12.8umol/L), more ingestion amount of GA (103.6±63.4ml vs 72.3±37.7ml) and higher-level CK-MB (43.5 U/L vs 16.0 U/L) compared to favorable outcomes groups. The area under the curve in the ROC analysis for the predictive variables, initial ammonia and CK-MB, were 0.873 and 0.680.The patients with GA poisoning may exhibit clinical symptoms of gastrointestinal and neurological toxicity, as well as respiratory failure which experience different latent period that we need to pay special attention to. Initial serum and CK-MB might be the most accurate prognostic factors for predicting the unfavorable outcomes.
Association between PM10 and respiratory diseases admission in Peninsular Malaysia during the haze
Authors: Zulkifli Siti Wafiah Hanin Mohd , Samsudin Humaida Banu , Majid Noriza 
Published: June 2023

Abstract

Numerous studies have been conducted in other countries on the health effects of exposure to particulate matter with a diameter of 10 microns or less PM10 , but little research has been conducted in Malaysia, particularly during the haze season. This study intends to investigate how exposure of PM10 influenced hospital admissions for respiratory diseases during the haze period in peninsular Malaysia and it was further stratified by age group, gender and respiratory diseases categories. The study includes data from all patients with respiratory diseases in government hospitals, as well as PM10 concentration and meteorological data from all monitoring stations in peninsular Malaysia starting from 1st January 2000 to 31st December 2019. A quasi-poison time series regression with distributed lag nonlinear model (DLNM) was employed in this study to examine the relationship between exposure of PM10 and hospital admissions for respiratory diseases during the haze period. According to the findings of this study, PM10 was positively associated with respiratory disease admissions within 30 lag days under various lag patterns, with lag 25 showing the strongest association (RR = 1.001742,CI : 1.001029,1.002456). Using median as a reference, it was discovered that females were more likely than males to be hospitalized for PM10 exposure. Working age group will be the most affected by the increase in PM10 exposure with a significant cumulative RR from lag 010 to lag 030. The study found that PM10 had a significant influence on respiratory hospitalisation in peninsular Malaysia, particularly for lung diseases caused by external agents(CD5). Therefore, it is important to implement effective intervention measures to control PM10 and reduce the burden of respiratory disease admissions
COVID-19 Distress, Parenting Stress, Maladaptive Parenting and Children’s Problematic Media Use in China: Evidence from 2022 Spring in Shanghai
Authors: Wang Jingyao , Wang Shumin , Xiao Bowen , Li Juan , Feng Yuemin , Li Yan 
Published: June 2023

Abstract

The COVID-19 lockdowns have forced young children to spend more time in front of the media and significantly impacted their mothers' mental health. This study explored how mothers' individual distress influences children's problematic media use during the Shanghai citywide lockdown caused by COVID-19. Data were collected from 1889 Chinese mothers (Mage= 34.69 years,SD = 3.94 years) with preschoolers aged 3–6 years (Mage= 4.38 years,SD = 1.06 years; 49.0% boys) via an online survey. The statistical analyses relied on SPSS Statistics version 26.0 and macro-program PROCESS 3.3. to investigate the associations and mediation analysis among all the study variables. The results indicated that: (1) significant associations between individual maternal distress with children's problematic media use; (2) maternal parenting stress and maladaptive parenting serial mediated the relationship between mothers' individual distress and children's problematic media use. The findings imply that parents need to enhance their ability to manage risk and promote mental health during periods of significant stress and routine disruption to reduce children's problematic media use.
Utility of fluorescence-guided biopsy in suspected lung cancer patients with bronchial mucosal lesions
Authors: Wang Gaozhe , Jin Yan , Xiong Kunlong , Jin Xiaoyan , Cao Chao 
Published: June 2023

Abstract
Background: Bronchoscopy is currently the most common technique for lung cancer diagnosis. Patients suspected of malignancy often undergo bronchoscopic examination, and biopsy is routinely used in patients with visible bronchial lesions. However, it is difficult to differentially diagnose lung cancer in patients with bronchial mucosal lesions. Thus, this study was conducted to investigate the utility of fluorescence-guided biopsy in suspected lung cancer patients withbronchial mucosal lesions.
Methods: We conducted a retrospective studyin a single screening center to assess the sensitivity and specificity of fluorescence-guided biopsy compared with white light bronchoscopy (WLB) in patients with bronchial mucosal lesions.
Results: A total of 301 patients with bronchial mucosal lesions were enrolled in this study. The sensitivity for patients with fluorescence-guided biopsy was 60.3% (95% confidence interval [CI]: 53.1%-67.1%), which was higher than that of patients with WLB alone (45.2%, 95% CI: 38.2-52.4%) (P= 0.0026). Additionally, compared with the WLB group, the fluorescence -guided biopsy group was found to have a significantly higher specificity (100%, 95% CI: 95.5-100% versus 69.6%, 95% CI: 59.6-78.1%), positive predictive value (100%, 95% CI: 96.1-100% versus 74.3%, 95% CI: 65.5-81.7%) and negative predictive value (56.3%, 95% CI: 48.8-63.6% versus 39.4%, 95% CI: 32.3-47.0%).
Conclusion: Fluorescence-guided biopsy can serve as an important adjunct to WLB for the differential diagnosis of lung cancer in patients with bronchial mucosal lesions.
How Perfectionism Determines Success or Failure: A Dual Pathway Mechanism Study on the Influence of Perfectionistic Climate on Performance of Elite Female Basketball Players in CUBAL
Authors: Meng Meng , Su Rong-Hai , Kogiso Kohei , Zheng Rong-Rong , Chen Lin , Wei Li , Hsu Mao-Chou 
Published: June 2023

Abstract
This study, based on the cognitive appraisal theory of stress, constructs a dual-pathway theoretical model called the Perfectionism-Performance Theory to examine the influence of perfectionistic climate on athletes' performance. The study empirically tests the relationships among the constructs in the model using a sample of 125 core players from the top-level women's basketball teams in the 24th CUBA L24 tournament in 2022. The findings reveal a double-edged effect of perfectionistic climate on athletes' performance. In the positive pathway, perfectionistic climate positively influences athletes' performance through challenge stressors and positive coping strategies. In the negative pathway, perfectionistic climate negatively affects athletes' performance through hindrance stressors and negative coping strategies. This study aims to enhance athletes' adaptation to perfectionistic climate and further improve individual and team performance.
Mitochondrial-derived Peptides: Are They Actually Important?
Authors: Gruschus James M. , Morris Daniel L. , Tjandra Nico 
Published: June 2023

Abstract
Mitochondrial-derived peptides are encoded by mitochondrial DNA but have biological activity outside mitochondria. Eight of these are encoded by sequences within the mitochondrial 12S and 16S ribosomal genes: humanin, MOTS-c, and the six SHLP peptides, SHLP1-SHLP6. These peptides have various effects in cell culture and animal models, affecting neuroprotection, insulin sensitivity, and apoptosis, and some are secreted, potentially having extracellular signaling roles. However, except for humanin, their importance in normal cell function is unknown. To gauge their importance, their coding sequences in vertebrates have been analyzed for synonymous codon bias. Because they lie in RNA genes, such bias should only occur if their amino acids have been conserved to maintain biological function. Humanin and SHLP6 show strong synonymous codon bias and sequence conservation. In contrast, SHLP1, SHLP2, SHLP3, and SHLP5 show no significant bias and are poorly conserved. MOTS-c, SHLP4 also lack significant bias, but contain highly conserved N-terminal regions, and their biological importance cannot be ruled out. An additional potential mitochondrial-derived peptide sequence was discovered preceding SHLP2, named SHLP2b, which also contains a highly conserved N-terminal region with synonymous codon bias.
Chlorfenapyr metabolism by mosquito P450s associated with pyrethroid resistance: identification of potential activation markers.
Authors: Yunta Cristina , Ooi Jocelyn , Oladepo Folasade , Grafanaki Sofia , Pergantis Spiros , Tsakireli Dmitra , Ismail Hanafy , Paine Mark 
Published: June 2023

Abstract
Chlorfenapyr is a pro-insecticide increasingly used in combination with pyrethroids such as a-cypermethrin or deltamethrin in insecticide treated bednets (ITNs) to control malaria transmitted by pyrethroid-resistant mosquito populations. Chlorfenapyr requires P450 activation to produce tralopyril and other bioactive metabolites. Pyrethroid resistance is often associated with elevated levels of chemoprotective P450s with broad substrate specificity, which could influence chlorfenapyr activity. Here, we have investigated chlorfenapyr metabolism by a panel of eight P450s commonly associated with pyrethroid resistance in An. gambiae and Ae. aegypti, the major vectors of malaria and arboviruses. Chlorfenapyr was activated to tralopyril by An. gambiae CYP6P3, CYP9J5, CYP9K1 and Ae. aegypti, CYP9J32. Kcat/ KM value of 0.66 µM-1 min-1, 6.7 fold higher than CYP6P3 and CYP9J32 (both 0.1 µM-1 min-1) and 22-fold higher than CYP9J5 (0.03 µM-1 min-1). Further investigation of the effect of a-cypermethrin equivalent to the ratios used with chlorfenapyr in bed nets (~ 1:2 molar ratio) resulted in a reduction in chlorfenapyr metabolism by CYP6P3 and CYP6K1 and of 76.8% and 56.8% respectively. This research provides valuable insights into the metabolism of chlorfenapyr by mosquito P450s and highlights the need for continued investigation into effective vector control strategies.
Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2
Authors: Liu Jiao , Zhu Jie , Zhang Qian , Lv Ruitong , Liu Hao 
Published: June 2023

Abstract
Objectives
To develop a one-step, marker-free CRISPR/Cas9 system for highly efficient genome editing in industrial Aspergillus niger, with a short genetic operation cycle.
Results
Firstly, evaluation of different promoters for sgRNA expression revealed tRNAGly15 as the most efficient, achieving a remarkable 100% gene editing efficiency. Furthermore, a counter-selectable marker, Ang-ace2, was identified for A. niger. Subsequently, a CRISPR/Cas9 plasmid was developed, utilizing a truncated AMA1 element and the Ang-ace2 conditional expression cassette driven by a Tet-on promoter. In the presence of doxycycline, the plasmid demonstrated a 33% loss efficiency in the progeny of A. niger spores after a single generation, resulting in a shortened genetic operation cycle of 16 days for CRISPR/Cas9.
Conclusions
The one-step marker-free CRISPR/Cas9 system was successfully developed in industrial A. niger, allowing for efficient gene editing while simultaneously reducing the editing time.