bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating explicit reliability for optimal choices: effect of trustworthiness on decisions and metadecisions
Authors: Ota Keiji , Gajdos Thibault , Ciston Anthony , Haggard Patrick , Charles Lucie 
Published: January 2025

AbstractIn everyday life, decision-makers need to integrate information from various sources which differ in how reliable the correct information is provided. The present study addressed whether people can optimally use explicit information about the trustworthiness of the information source and whether people know how information sources affect their decisions. In each trial, we presented a sequence of six information sources which indicated a correct colour (red or blue) each with a different level of reliability. Each source was explicitly labelled the reliability percentage, i.e., the likelihood that a source provides correct information. Participants were asked to decide which colour was more likely to be correct. In the first series of three experiments, we found that participants failed to make use of explicit reliability cues optimally. In particular, participants were less able to use reliably wrong information sources (reliability below 50%) than reliably correct information sources (reliability above 50%), even though these two types of information sources were equally informative. In addition, participants failed to ignore the colour displayed by unreliable sources (50% reliability), although these sources gave just random information for a binary decision. In the second series of two experiments, we asked participants, after each choice, to report their subjective feelings of whether they followed or opposed a colour suggested by sources with a specific reliability percentage. We found that the ratings of the participants’ influence report tracked the amount of evidence which supported the choice they just made. Further, participants were able to introspect their own choice bias guided by unreliable information sources. These findings suggest that human choice behaviour deviates from Bayesian integration. However, people have a good metacognitive monitoring of how their decisions are driven by external stimuli.
Ceratopteris richardiiU6 promoters and plants expressing Cas9 endonuclease as tools for efficient genome editing in a fern
Authors: Schulz Robin , Theißen Günter 
Published: January 2025

AbstractFerns and their allies (monilophytes) represent the second most species-rich group of land plants and are of considerable ecological importance. As a sister group of seed plants (including flowering plants) they are also of great evolutionary interest. Compared to flowering plants, however, much less is known about the developmental and molecular biology of ferns. Among the most important reasons have been the huge genome sizes of ferns and technical obstacles such as the lack of an efficient transformation system. In recent years the situation has improved considerably, however. For the fern model systemCeratopteris richardiia whole genome sequence has been published, and an efficient transformation system has been developed. To further facilitate studies on fern biology we aim at simplifying genome engineering ofC. richardiiwith the CRISPR-Cas9 system. We reportC. richardiiplants that express Cas9 nuclease under control of the strong CaMV 35S promoter. For efficient expression of single guide RNA (sgRNA) by RNA polymerase III we identifiedC. richardiiU6 promoters. These technical improvements may foster many fields of fern physiology, development and evolution.
Acoustic features of and behavioral responses to emotionally intense mouse vocalizations
Authors: Ghasemahmad Zahra , Perumal Karthic Drishna , Sharma Bhavya , Panditi Rishita , Wenstrup Jeffrey J. 
Published: January 2025

ABSTRACTSocial vocalizations contain cues that reflect the motivational state of a vocalizing animal. Once perceived, these cues may in turn affect the internal state and behavioral responses of listening animals. Using the CBA/CAJ mouse model of acoustic communication, this study examined acoustic cues that signal intensity in male-female interactions, then compared behavioral responses to intense mating vocal sequences with those from another intense behavioral context, restraint. Experiment I in this study examined behaviors and vocalizations associated with male-female social interactions. Based on several behaviors, we distinguished more general, courtship-type interactions from mating interactions involving mounting or attempted mounting behaviors. We then compared vocalizations between courtship and mating. The increase in behavioral intensity from courtship to mating was associated with altered syllable composition, more harmonic structure, lower minimum frequency, longer duration, reduced inter-syllable interval, and increased sound intensity. We then used these features to construct highly salient playback stimuli associated with mating. In Experiment II, we compared behavioral responses to playback of these mating sequences with responses to playback of aversive vocal sequences produced by restrained mice, described in previous studies. Subjects were females in estrus and males. We observed a range of behavioral responses. Some (e.g., Attending and Stretch-Attend) showed similar responses across playback type and sex, while others were context dependent (e.g., Flinching, Locomotion). Still other behaviors showed either an effect of sex (e.g., Self-Grooming, Still-and-Alert) or an interaction between playback type and sex (Escape). These results demonstrate both state-dependent features of mouse vocalizations and their effectiveness in evoking a range of behavioral responses, independent of contextual cues provided by other sensory stimuli or behavioral interactions.
Post menopausal women with Musculoskeletal disorder (MSKDs) and its relationship with regular dietary habit
Authors: Islam Md Faruqul , Faruque Md. Omar , Alauddin Md. , Kabir Md Feroz , Moula Golam , Hossian Akter , Bin Sultan Zahid , Afridi Shahid , Islam Md Waliul 
Published: January 2025

AbstractObjectivesto examine the association between consistent dietary patterns and musculoskeletal diseases (MSKDs) in postmenopausal women.MethodsA descriptive cross-sectional study recruited 576 women aged 42 to 70 years who received treatment at the Centre for the Rehabilitation of the Paralyzed (CRP), specifically the Musculo-Skeletal Department, located in Savar, CRP-Dhaka Mirpur Centre, and Unique Pain and Paralysis Centre (UPPC), Dhaka Mirpur, Bangladesh during the period from December 2022 to December 2023. The musculoskeletal disorders (MSKDs) were determined by Nordic MSK Questionnaire (NMQ) and regular dietary habit was determined by the 24-hour Dietary consumption Questionnaire. Data were collected by face-to-face interviews on demographic factors, medical history, menopausal state, and symptoms. Correlation models were used to identify factors associated between MSKDs and dietary habit.ResultsAmong all participants, the age was categorized in several years and no one experiencing menstrual cycle in last 3 month. The mean overweight and obese BMI were 38.50% and 9.20%. All most all participants were feeling chronic pain on various part of body last 12 months and mean pain rating were 6.111.321. As well as dietary intake were calculation of each individual throughout a 24-hour period. The chronic MSKDs and protein was associated with upper back(p=0.046), carbohydrate was associated with LBP (p=0.005), hip pain (0.008) and knee pain (0.002) while fat was associated with shoulder pain (p=0.007*), wrist pain (p=0.002*), hip pain (p=0.044*), knee pain (p=0.002*), and ankle pain (p=0.010*) but acute pain was only associated with carbohydrate (p=0.012) which had significant.ConclusionPost menopausal women with acute MSKDs had suchlike no relation with regular dietary habit but a substantial association was seen with some chronic MSKDs.
Genetic contributions to premenstrual symptoms: revisiting the role of theESR1gene
Authors: Lahnsteiner Angelika , Hidalgo-Lopez Esmeralda , Noachtar Isabel , Hausinger Tobias , Gnaiger Anna , Griesbach Katrin , Scutelnic Diana , Risch Angela , Pletzer Belinda Angela 
Published: January 2025

AbstractPremenstrual dysphoric disorder (PMDD) and its subclinical form categorized as premenstrual syndrome (PMS), are severe mood disorders characterized by cyclical depressive symptoms, anxiety, irritability, and other functional impairments, impacting a broad range of women during the late luteal phase. The estrogen receptor 1 (ESR1)gene encodes the estrogen receptor alpha (ERα) which plays a critical role in mediating estrogen signaling and regulates various physiological and psychological processes. In this study, we investigated the relationship between six single nucleotide polymorphisms (SNPs) in intron 4 of theESR1gene and premenstrual symptom severity, emphasizing symptom- genotype associations and SNP interactions. Results demonstrated that specific SNPs were linked to distinct symptom profiles, such as anxiety, difficulty concentrating, and sleep disturbances. Interactions between SNPs revealed both risk-enhancing and protective effects. These findings suggest that premenstrual symptoms may stem from a genotype-linked reduced sensitivity to ovarian hormones, providing a foundation for future research.
Sex Differences in Endocannabinoid and Inflammatory Markers Associated with Posttraumatic Stress Disorder
Authors: Rajasekera Therese A. , Joseph Anna , Pan Hui , Dreyfuss Jonathan M. , Fida Doruntina , Wilson Julie , Behee Madeline , Fichorova Raina N , Cinar Resat , Spagnolo Primavera A. 
Published: January 2025

AbstractBackgroundPosttraumatic stress disorder (PTSD) is one of the most sex-polarized psychiatric disorders, with women exhibiting twice the prevalence of men. The biological mechanisms underlying this sex disparity are not fully understood. Growing evidence suggests that alterations of the stress-buffering endocannabinoid (eCB) system and heightened inflammation are central to PTSD pathophysiology and may contribute to sex-biases in PTSD risk and severity. Here, we examined sex-differences in levels of circulating eCBs and peripheral pro-inflammatory markers in a cohort of men and women with PTSD and non-psychiatric controls.Methods88 individuals with PTSD and 85 sex- and age-matched controls (HC) were retrospectively selected from the Mass General Brigham Biobank. Serum was assayed to measure circulating eCBs [anandamide (AEA), 2-arachidonyl glycerol (2-AG), oleoylethanolamide (OEA), and arachidonic acid (AA] and inflammatory markers [interleukin-1β (IL-1β), IL-6, IL-8, IL-18, tumor necrosis factor-alpha (TNFα), and C-reactive Protein (CRP)]. Linear regression was used to predict differential abundance of each analyte by disease state (PTSD/HC) within the male and female subgroups. Two-sided t-tests with Benjamini-Hochberg correction were used to examine differences across subgroups. Analyses were repeated in those with comorbid major depressive disorder.ResultsMale PTSD patients showed a significant decrease in AEA, AA and OEA levels compared to male controls (p’s < .001) and to female subgroups (PTSD and HCs) (p< .001). In contrast, among females, PTSD patients showed elevated levels of IL-6 (p<.05) and IL-8 (p<.05). Both male and female PTSD patients exhibited an increase in TNFα concentrations (p<.05), compared to HCs. Similar results were obtained in the subgroup of individuals with comorbid MDD and after controlling for theFAAH385A genotype.ConclusionsOur findings show for the first time that decrease in eCB levels is absent in women with PTSD, who in turn exhibit a broader increase in inflammatory markers, thus suggesting that biological perturbations underlying PTSD may vary by sex.
Incidence and predictors of pulmonary exacerbations in primary ciliary dyskinesia: an international prospective cohort study
Authors: Schreck Leonie D , Goutaki Myrofora , Pedersen Eva SL , Copeland Fiona , Fernández Trini López ,   Living with PCD patient advisory group, Lucas Jane S , Kuehni Claudia E 
Published: January 2025

AbstractIntroductionPulmonary exacerbations contribute to disease progression in chronic lung diseases. In a large prospective cohort study, we studied the incidence and predictors of pulmonary exacerbations among persons with primary ciliary dyskinesia (PCD), which can inform follow-up care. We also assessed healthcare use, changes in management, and pathogens during exacerbations.MethodsParticipants in theLiving with PCDstudy reported increased respiratory symptoms in the past seven days, indicating a pulmonary exacerbation, from June 2020 through May 2022 via online questionnaires. We derived incidence rates and studied predictors of pulmonary exacerbation incidence by fitting multivariable negative binomial regression models.ResultsWe obtained data from 660 persons (408 adults, 57 adolescents, 195 children) who completed 17,853 follow-up questionnaires (median 17, range 1-84). The 1026 reported exacerbations indicate an incidence rate of 3.1 pulmonary exacerbations per person per year, with minor variation across age groups, but changes over time. Incidence was higher among adult females [incidence rate ratio (IRR) 2.0, 95% confidence interval (CI) 1.4-2.7] and those in whomPseudomonas aeruginosawas isolated (children IRR 1.9, 95% CI 1.1-3.6; adults IRR 1.4, 95% CI 1.0-1.9). Participants saw a health professional during only 185 of 1404 exacerbation weeks (13%).Pseudomonas aeruginosawas the pathogen most frequently observed during exacerbations in children (18 of 118 samples, 15%) and adults (132 of 303 samples, 44%).ConclusionPulmonary exacerbations are frequent in PCD and heighten the disease burden. Patients for whom targeted management is particularly important include adult females and those who carryPseudomonas aeruginosa.
A Mitochondrial Supplement Improves Function and Mitochondrial Activity in Autism: A double-blind placebo-controlled cross-over trial
Authors: Hill Zoë , McCarty Patrick J , Boles Richard G. , Frye Richard E. 
Published: January 2025

AbstractAutism spectrum disorder (ASD) is associated with mitochondrial dysfunction but studies demonstrating the efficacy of treatments are scarce. We sought to determine whether a mitochondrial-targeted dietary supplement designed for children with ASD improved mitochondrial function and ASD symptomatology using a double-blind placebo-controlled cross-over design. Sixteen children [Mean Age 9y 4m; 88% male] with non-syndromic ASD and mitochondrial enzyme abnormalities, as measured by MitoSwab (Religen, Plymouth Meeting, PA), received weight-adjusted SpectrumNeeds®(NeuroNeeds, Old Lyme, CT) and QNeeds®(NeuroNeeds) and placebos matched on taste, texture and appearance during two separate 12-week blocks. Which product received first was randomized. The treatment significantly normalized citrate synthase and complex IV activity as measured by the MitoSwab. Mitochondrial respiration of peripheral blood mononuclear cell respiration, as measured by the Seahorse XFe96 (Agilent, Santa Clara, CA) with the mitochondrial oxidative stress test, became more resilient to oxidative stress after the treatment, particularly in children with poor neurodevelopment. The mitochondrial supplement demonstrated significant improvement in standardized parent-rated scales in neurodevelopment, social withdrawal, and hyperactivity with large effect sizes (Cohen’s d’ = 0.77-1.25), while changes measured by the clinical and psychometric instruments were not significantly different. Adverse effects were minimal. This small study on children with ASD and mitochondrial abnormalities demonstrates that a simple, well-tolerated mitochondrial-targeted dietary supplement can improve mitochondrial physiology, ASD symptoms. Further larger controlled studies need to verify and extend these findings. These findings are significant as children with ASD have few other effective treatments.
Eye Tracking during Passage Reading Supports Precise Oculomotor Assessment in Ataxias
Authors: Oubre Brandon , Yang Faye , Luddy Anna C. , Manohar Rohin , Soja Nancy N. , Stephen Christopher D. , Schmahmann Jeremy D. , Kulkarni Divya , White Lawrence , Patel Siddharth , Gupta Anoopum S. 
Published: January 2025

AbstractAbnormal eye movements occur early in the course of disease in many ataxias. However, clinical assessments of oculomotor function lack precision, limiting sensitivity for measuring progression and the ability to detect subtle early signs. Quantitative assessment of eye movements during everyday behaviors such as reading has potential to overcome these limitations and produce functionally relevant measures.In this study, we analyze eye movements in individuals with ataxia during passage reading. Binocular gaze sampled at 1000 Hz was collected from 102 individuals with ataxia diagnoses (including 36 spinocerebellar ataxias, 12 Friedreich’s ataxia, and 5 multiple system atrophy among other conditions) and 70 healthy controls participating in the Neurobooth study. Longitudinal data were available for 26 participants with ataxia. Saccades were categorized as progressive (rightward) saccades, regressive saccades, or sweeps (large displacement saccades primarily generated when scanning to the beginning of the next line) based on their direction and displacement. Saccade and fixation kinematics were summarized using 28 statistical features. A linear model was trained to estimate clinician-performed ataxia rating scale scores.Model scores were reliable (ICC=0.96, p<0.001) and demonstrated convergent validity with Brief Ataxia Rating Scale total (r=0.82, p<0.001), oculomotor (r=0.52, p<0.001), and speech (r=0.73, p<0.001) scores, as well as patient surveys. The scores were also sensitive to disease progression (d=0.36, p=0.03), demonstrated strong separability between healthy controls and participants with ataxias (AUC=0.89, p<0.001), and showed evidence of the ability to detect subclinical oculomotor patterns (AUC=0.69, p=0.02). Several kinematic saccade and fixation features demonstrated strong differences across disease severity groups. Notable features included the mean angular displacement of fixations (η2=0.44, p<0.001), the number (η2=0.27, p<0.001) and frequency of saccades (η2=0.25, p<0.001), and the proportion of regressive saccades (η2=0.11, p<0.001).Quantitative assessment of eye movements during passage reading were highly informative of ataxia severity, were sensitive to disease progression, and enabled detection of subclinical signs. These properties support the inclusion of video-oculography-based measures of reading in natural history studies and clinical trials. Furthermore, this study demonstrates the feasibility of integration of oculomotor assessments in clinical workflows.Abstract Figure
Tau Burden is Best Captured by Magnitude and Extent: Tau-MaX as a Measure of Global Tau
Authors: Brown Christopher A. , Das Sandhitsu R. , Cousins Katheryn A.Q. , Tropea Thomas F. , Plotkin Alice-Chen , Detre John A. , Yushkevich Paul A. , McMillan Corey T. , Lee Edward B. , Shaw Leslie M. , Nasrallah Ilya M. ,   the Alzheimer’s Disease Neuroimaging Initiative, Wolk David A. 
Published: January 2025

AbstractTau exhibits change in both spatial extent and density of pathology along the Alzheimer’s disease (AD) spectrum with each aspect contributing to the overall burden of pathological tau. Nevertheless, studies using Tau PET have measured either magnitude using standardized uptake value ratios (SUVRs) or extent using number of Tau+ regions. We hypothesized that combining these two dimensions into a single measure of Magnitude and eXtent, Tau-MaX, would provide improved quantification of global tau burden as well as allowing for a region-agnostic measure of global tau burden that does not require a pre-specified region of interest (ROI) or meta-ROI. To test this hypothesis, we analyzed18F-flortaucipir PET scans from local and national consortium data (n=1077 participants total) and used Gaussian-mixture models for data from 64 brain regions, to define both tau positivity and magnitude. We examined cross-sectional and longitudinal change in Tau-MaX across the Alzheimer’s disease (AD) spectrum and compared the association of Tau-MaX, magnitude, and extent with plasma p-tau217and global cognition. We also compared Tau-MaX using a global, region-agnostic approach to temporal lobe or Braak stage meta-ROIs. Whereas separate assessments of extent and magnitude across the disease spectrum found earlier increases in Tau spatial extent and later increases in magnitude, Tau-MaX was able to dynamically capture this shift demonstrating a stronger association with extent in the preclinical stage and a stronger association with magnitude in clinical stages. Global Tau-MaX differed between disease stages cross-sectionally and changed over time in all stages of disease. Further, Tau-MaX significantly improved associations with plasma p-tau217and global cognition compared to magnitude or extent alone. Finally, global measures of Tau-MaX performed similarly to meta-ROI measures of Tau-MaX. Together, these findings indicate that combining magnitude and extent provides a robust measure of global tau burden that changes throughout the disease course and is associated with blood-based biomarkers and cognition. This measure may be of particular use for disease staging, as well as serving as an outcome measure to monitor response to therapeutic intervention.
Expression of clock genes tracks daily and tidal time in brains of intertidal crustaceansEurydice pulchraandParhyale hawaiensis
Authors: Oliphant A. , Sia C.Y. , Kyriacou C.P. , Wilcockson D.C. , Hastings M.H. 
Published: January 2025

AbstractIntertidal organisms, such as the crustaceansEurydice pulchraandParhyale hawaiensis, express daily and tidal rhythms of physiology and behaviour to adapt to their temporally complex environments. Although the molecular-genetic basis of the circadian clocks driving daily rhythms in terrestrial animals is well understood, the nature and mechanism of the circatidal clocks driving tidal rhythms remain a mystery. Usingin situhybridisation, we identified discrete clusters of ∼60 putative “clock” cells co-expressing canonical circadian clock genes with comparable distributions across the protocerebrum ofE. pulchraandP. hawaiensisbrains. In tidally rhythmic, field-collectedE. pulchrasampled under a light:dark (LD) cycle, the expression ofperiod(per) andcryptochrome 2(cry2) exhibited daily rhythms in particular cell groups (e.g., medioposterior cells) whereastimeless(tim) showed 12-hour rhythms in others (e.g., medial cells). In tidally rhythmic laboratory-rearedP. hawaiensis, previously entrained to 12.4-hour cycles of agitation under LD and sampled under continuous darkness, several cell groups (e.g., medioposterior cells) exhibited circadian expression ofperandcry2. In contrast, dorsal-lateral cells in the protocerebrum exhibited robust ∼12-hour, i.e., circatidal, rhythms ofperandcry2, phased to the prior tidal agitation but not the prior LD. InP. hawaiensisexhibiting daily behaviour under LD without tidal agitation, robust daily rhythms ofperandcry2expression were evident in medioposterior and other cells whereas expression in dorsal-lateral cells was not rhythmic, underlining their intrinsic tidal periodicity. These results implicate canonical circadian mechanisms in circatidal time-keeping and reveal conserved brain networks as potential neural substrates for the generation of interactive daily and tidal rhythms appropriate to intertidal habitats.