bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Glyco-sulfo barcodes” regulate chemokine receptor function
Authors: Verhallen Lisa , Lackman Jarkko J. , Wendt Rikke , Gustavsson Martin , Yang Zhang , Narimatsu Yoshiki , Sørensen Daniel M. , Lafferty Kato Mac , Gouwy Mieke , Marques Pedro E. , Hjortø Gertrud M. , Rosenkilde Mette M. , Proost Paul , Goth Christoffer K. 
Published: February 2023

AbstractChemokine ligands and receptors regulate the directional migration of leukocytes. Post-translational modifications of chemokine receptors including O-glycosylation and tyrosine sulfation have been reported to regulate ligand binding and resulting signaling. Through in silico analyses, we determined potential conserved O-glycosylation and sulfation sites on human and murine CC chemokine receptors. Glyco-engineered CHO cell lines were used to measure the impact of O-glycosylation on CC chemokine receptor CCR5, while mutation of tyrosine residues and treatment with sodium chlorate were performed to determine the effect of tyrosine sulfation. Changing the glycosylation or tyrosine sulfation on CCR5 reduced the receptor signaling by the more positively charged CCL5 and CCL8 more profoundly compared to the less charged CCL3. The loss of negatively charged sialic acids resulted only in a minor effect on CCL3-induced signal transduction. The enzymes GalNAc-T1 and GalNAc-T11 were shown to be involved in the process of chemokine receptor O-glycosylation. These results indicate that O-glycosylation and tyrosine sulfation are involved in the fine-tuning and recognition of chemokine interactions with CCR5 and the resulting signaling.
Effect of ATG12–ATG5-ATG16L1 autophagy E3-like complex on the ability of LC3/GABARAP proteins to induce vesicle tethering and fusion
Authors: Iriondo Marina N. , Etxaniz Asier , Varela Yaiza R. , Ballesteros Uxue , Lázaro Melisa , Valle Mikel , Fracchiolla Dorotea , Martens Sascha , Montes L. Ruth , Goñi Félix M. , Alonso Alicia 
Published: February 2023

AbstractIn macroautophagy, the autophagosome (AP) engulfs portions of cytoplasm to allow their lysosomal degradation. AP formation in humans requires the concerted action of the ATG12 and LC3/GABARAP conjugation systems. The ATG12–ATG5-ATG16L1 or E3-like complex (E3 for short) acts as a ubiquitin-like E3 enzyme, promoting LC3/GABARAP proteins anchoring to the AP membrane. Their role in the AP expansion process is still unclear, in part because there are no studies comparing six LC3/GABARAP family member roles under the same conditions, and also because the full human E3 was only recently available. In the present study, the lipidation of six members of the LC3/GABARAP family has been reconstituted in the presence and absence of E3, and the mechanisms by which E3 and LC3/GABARAP proteins participate in vesicle tethering and fusion have been investigated. In the absence of E3, GABARAP and GABARAPL1 showed the highest activities. Differences found within LC3/GABARAP proteins suggest the existence of a lipidation threshold, lower for the GABARAP subfamily, as a requisite for tethering and inter-vesicular lipid mixing. E3 increases and speeds up lipidation and LC3/GABARAP-promoted tethering. However, E3 hampers LC3/GABARAP capacity to induce inter-vesicular lipid mixing or subsequent fusion, presumably through the formation of a rigid scaffold on the vesicle surface. Our results suggest a model of AP expansion in which the growing regions would be areas where the LC3/GABARAP proteins involved should be susceptible to lipidation in the absence of E3, or else a regulatory mechanism would allow vesicle incorporation and phagophore growth when E3 is present.
Künstliche Intelligenz in der Urologie – Chancen und Möglichkeiten
Authors: Alexa Radu , Kranz Jennifer , Kuppe Christoph , Hayat Sikander , Hoffmann Marco , Saar Matthias 
Published: February 2023

ZusammenfassungDer Einsatz künstlicher Intelligenz (KI) in der Urologie soll zu einer deutlichen Optimierung und Individualisierung der Diagnostik und Therapie sowie einer Kostenreduktion für das Gesundheitssystem beitragen. Die Einsatzmöglichkeiten und Vorteile der KI im medizinischen Bereich werden oftmals unterschätzt oder nur unvollständig verstanden. Dadurch wird die konzeptuelle Lösung von relevanten medizinischen Problemen mit Hilfe von KI-Anwendungen erschwert. Mit den aktuellen Fortschritten der Computerwissenschaften wurden bereits multiple, teils hochkomplexe nicht medizinische Prozesse automatisiert untersucht und optimiert. Die konstante Entwicklung von KI-Modellen kann bei korrekter Anwendung zu einer effektiveren Bearbeitung und Analyse patientenbezogener Daten und einer dementsprechend optimierten Diagnostik und Therapie urologischer Patientinnen und Patienten führen. In dieser Übersichtsarbeit wird der aktuelle Status zur Anwendung der KI in der Medizin sowie deren Chancen und Möglichkeiten in der Urologie aus einer konzeptuellen Perspektive anhand praktischer Beispiele dargestellt.
Reference Intervals for Bone Impact Microindentation in Healthy Adults: A Multi-Centre International Study
Authors: Rufus-Membere Pamela , Holloway-Kew Kara L. , Diez-Perez Adolfo , Appelman-Dijkstra Natasha M. , Bouxsein Mary L. , Eriksen Erik F. , Farr Joshua N. , Khosla Sundeep , Kotowicz Mark A. , Nogues Xavier , Rubin Mishaela , Pasco Julie A. 
Published: February 2023

AbstractImpact microindentation (IMI) is a novel technique for assessing bone material strength index (BMSi) in vivo, by measuring the depth of a micron-sized, spherical tip into cortical bone that is then indexed to the depth of the tip into a reference material. The aim of this study was to define the reference intervals for men and women by evaluating healthy adults from the United States of America, Europe and Australia. Participants included community-based volunteers and participants drawn from clinical and population-based studies. BMSi was measured on the tibial diaphysis using an OsteoProbe in 479 healthy adults (197 male and 282 female, ages 25 to 98 years) across seven research centres, between 2011 and 2018. Associations between BMSi, age, sex and areal bone mineral density (BMD) were examined following an a posteriori method. Unitless BMSi values ranged from 48 to 101. The mean (± standard deviation) BMSi for men was 84.4 ± 6.9 and for women, 79.0 ± 9.1. Healthy reference intervals for BMSi were identified as 71.0 to 97.9 for men and 59.8 to 95.2 for women. This study provides healthy reference data that can be used to calculate T- and Z-scores for BMSi and assist in determining the utility of BMSi in fracture prediction. These data will be useful for positioning individuals within the population and for identifying those with BMSi at the extremes of the population.
Safety of Continuing Anticoagulation Prior to Cardiac Catheterization in Pediatric Patients: A Los Angeles Center Experience
Authors: Rao Mounica Y. , Sullivan Patrick M. , Takao Cheryl , Badran Sarah , Patel Neil D. 
Published: February 2023

AbstractThis is the first report specifically describing outcomes of pediatric patients who underwent cardiac catheterization while on uninterrupted anticoagulation. One hundred forty-four cardiac catheterizations were identified that met inclusion criteria at our institution from 11/2014 to 10/2019. The median age and weight of the patients were 6.3 [0.01–20.9] years and 19.3 [2.1–172.5] kg, respectively. Seventy-eight (54%) catheterizations involved inpatients. The most common cardiac diagnoses among the cohort were single ventricle (n = 41), conotruncal defects (n = 37), and structurally normal heart (n = 16). The most common indications for anticoagulation were arterial/venous thrombus (n = 45), Fontan physiology (n = 32), and mechanical valve thrombus prophylaxis (n = 27). The anticoagulation medications used were warfarin (n = 57), heparin (n = 52), enoxaparin (n = 25), fondaparinux (n = 5), rivaroxaban (n = 2), and both heparin and warfarin (n = 3). Interventions were performed in 96 cases (67%). The median length of the procedure was 122.5 [15–760] minutes, and the median time to achieve hemostasis was 18.0 [range: 5–76, IQR: 13–25] minutes. Adverse events were present in 11 cases (7.6%), and of those only 2 cases (1.4%) were bleeding-related complications. Our single-center data suggest that performing cardiac catheterization on pediatric patients while on uninterrupted anticoagulation is safe and does not substantially increase the risk of bleeding complications based on a cohort of patients that varied in age, size, diagnosis, medical complexity, and type of intervention performed. Patients on warfarin therapy for a mechanical valve are most likely to benefit from this practice, as the ability to continue warfarin therapy avoids the need for bridging and other interruption-related complications.
Energy content of krill and amphipods in the Barents Sea from summer to winter: variation across species and size
Authors: Nowicki Robynne C. , Borgå Katrine , Gabrielsen Geir Wing , Varpe Øystein 
Published: February 2023

AbstractArctic zooplankton develop large energy reserves, as an adaptation to strong seasonality, making them valuable prey items. We quantified the energy content (kJ g−1 dry weight) of abundant krill (arcto-boreal, Thysanoessa inermis and boreal, Meganyctiphanes norvegica) and amphipods (Arctic, Themisto libellula and sub-Arctic-boreal, Themisto abyssorum) in the Barents Sea in late summer (August) and early winter (December). Variation in energy content was attributed to species-specific traits and body size categories, the latter in part as a proxy for ontogeny. T. inermis had the highest energy content, (Aug: 26.8 ± 1.5 (SD) kJ g−1) and remained similar from summer to winter. Energy content increased in M. norvegica and decreased in both amphipod species, with the lowest energy content being in T. abyssorum (Dec: 17.8 ± 0.8 kJ g−1). The effect of body size varied between species, with energy content increasing with size in T. inermis and T. libellula, and no change with size in M. norvegica and T. abyssorum. The reproductive stages of T. libellula differed in energy content, being highest in gravid females. Energy content varied with species’ dependence on energy storage. Our findings highlight how phylogenetically and morphologically similar prey items cannot necessarily be considered equal from a predator´s perspective. Energetically, the northern T. inermis was higher quality compared to the more southern M. norvegica, and mostly so during summer. Ecological models and management strategies should consider such variation in prey quality, especially as Arctic borealization is expected to change species composition and the energetic landscape for predators.
Radiation dose optimization for photon-counting CT coronary artery calcium scoring for different patient sizes: a dynamic phantom study
Authors: Dobrolinska Magdalena M. , van der Werf Niels R. , van der Bie Judith , de Groen Joël , Dijkshoorn Marcel , Booij Ronald , Budde Ricardo P. J. , Greuter Marcel J. W. , van Straten Marcel 
Published: February 2023

Abstract
Purpose
To systematically assess the radiation dose reduction potential of coronary artery calcium (CAC) assessments with photon-counting computed tomography (PCCT) by changing the tube potential for different patient sizes with a dynamic phantom.

Methods
A hollow artery, containing three calcifications of different densities, was translated at velocities corresponding to 0, < 60, 60–75, and > 75 beats per minute within an anthropomorphic phantom. Extension rings were used to simulate average- and large -sized patients. PCCT scans were made with the reference clinical protocol (tube potential of 120 kilovolt (kV)), and with 70, 90, Sn100, Sn140, and 140 kV at identical image quality levels. All acquisitions were reconstructed at a virtual monoenergetic energy level of 70 keV. For each calcification, Agatston scores and contrast-to-noise ratios (CNR) were determined, and compared to the reference with Wilcoxon signed-rank tests, with p < 0.05 indicating significant differences.

Results
A decrease in radiation dose (22%) was achieved at Sn100 kV for the average-sized phantom. For the large phantom, Sn100 and Sn140 kV resulted in a decrease in radiation doses of 19% and 3%, respectively. Irrespective of CAC density, Sn100 and 140 kVp did not result in significantly different CNR. Only at Sn100 kV were there no significant differences in Agatston scores for all CAC densities, heart rates, and phantom sizes.

Conclusion
PCCT at tube voltage of 100 kV with added tin filtration and reconstructed at 70 keV enables a ≥ 19% dose reduction compared to 120 kV, independent of phantom size, CAC density, and heart rate.

Key Points
• Photon-counting CT allows for reduced radiation dose acquisitions (up to 19%) for coronary calcium assessment by reducing tube voltage while reconstructing at a normal monoE level of 70 keV.
• Tube voltage reduction is possible for medium and large patient sizes, without affecting the Agatston score outcome.
Localization and tracking of stationary users for augmented reality
Authors: Baker Lewis , Ventura Jonathan , Langlotz Tobias , Gul Shazia , Mills Steven , Zollmann Stefanie 
Published: February 2023

AbstractIn augmented reality applications it is essential to know the position and orientation of the user to correctly register virtual 3D content in the user’s field of view. For this purpose, visual tracking through simultaneous localization and mapping (SLAM) is often used. However, when applied to the commonly occurring situation where the users are mostly stationary, many methods presented in previous research have two key limitations. First, SLAM techniques alone do not address the problem of global localization with respect to prior models of the environment. Global localization is essential in many applications where multiple users are expected to track within a shared space, such as spectators at a sporting event. Secondly, these methods often assume significant translational movement to accurately reconstruct and track from a local model of the environment, causing challenges for many stationary applications. In this paper, we extend recent research on Spherical Localization and Tracking to support relocalization after tracking failure, as well as global localization in large shared environments, and optimize the method for operation on mobile hardware. We also evaluate various state-of-the-art localization approaches, the robustness of our visual tracking method, and demonstrate the effectiveness of our system in real-life scenarios.
Feasibility and preliminary effectiveness of a psychosocial support program for adolescent and young adult cancer patients in clinical practice: a retrospective observational study
Authors: Hirayama Takatoshi , Fujimori Maiko , Ito Yoshinori , Ishida Yuji , Tsumura Akemi , Ozawa Miwa , Maeda Naoko , Yamamoto Kazuhito , Takita Sakie , Mori Makiko , Tanaka Kyoko , Horibe Keizo , Akechi Tatsuo 
Published: February 2023

Abstract
Purpose
Adolescent and young adult cancer patients (AYAs) often experience profound psychological distress, with various unmet supportive care needs that can be alleviated with appropriate screening and attention by healthcare workers. The Distress Thermometer and Problem List-Japanese version (DTPL-J) is our previously developed screening tool to facilitate individual support of AYAs. This study evaluated the feasibility and preliminary effectiveness of a psychosocial support program based on the DTPL-J for AYAs in clinical practice.

Methods
This multicenter, retrospective, observational study included 19 of 126 wards and 9 of 75 outpatient clinics at 8 institutions in Japan. Over 200 patients were expected to participate during the eligibility period. Patients participated in a support program at least once, and approximately once a month based on the DTPL-J results. The program was evaluated using the RE-AIM (Reach, Effectiveness, Adoption, Implementation, and Maintenance) implementation framework.

Results
The screening rate of the 361 participants was 90.3%, suggesting high feasibility. Distress Thermometer scores, the number of supportive care needs, and the rates of AYAs with high distress were significantly reduced 1 month after screening (p < 0.05), suggesting the preliminary effectiveness of the program. The program was continued at the 8 institutions as part of routine care after the study.

Conclusion
Analysis using the RE-AIM suggested the sufficient feasibility and preliminary effectiveness of a psychosocial support program based on the DTPL-J for AYAs.

Trial registration
University Hospital Medical Information Network (UMIN CTR) UMIN000042857. Registered 25 December 2020—Retrospectively registered.
Constant rank theorems for curvature problems via a viscosity approach
Authors: Bryan Paul , Ivaki Mohammad N. , Scheuer Julian 
Published: February 2023

AbstractAn important set of theorems in geometric analysis consists of constant rank theorems for a wide variety of curvature problems. In this paper, for geometric curvature problems in compact and non-compact settings, we provide new proofs which are both elementary and short. Moreover, we employ our method to obtain constant rank theorems for homogeneous and non-homogeneous curvature equations in new geometric settings. One of the essential ingredients for our method is a generalization of a differential inequality in a viscosity sense satisfied by the smallest eigenvalue of a linear map Brendle et al. (Acta Math 219:1–16, 2017) to the one for the subtrace. The viscosity approach provides a concise way to work around the well known technical hurdle that eigenvalues are only Lipschitz in general. This paves the way for a simple induction argument.
Compensatory mechanisms from different exercise intensities in type 2 diabetes: a secondary analysis of a 1-year randomized controlled trial
Authors: Correia Inês R. , Hetherington-Rauth Megan , Magalhães João P. , Júdice Pedro B. , Rosa Gil B. , Henriques-Neto Duarte , Manas Asier , Ara Ignacio , Silva Analiza M. , Sardinha Luís B. 
Published: February 2023

Abstract
Aims
This investigation aimed to determine the effect of different intensities of training on non-exercise physical activity (NEPA) and estimated thermogenesis (NEAT) from a 1-year exercise randomized controlled trial (RCT) in individuals with type 2 diabetes mellitus (T2DM) on non-training days. Additionally, changes in NEPA and estimated NEAT in those who failed (low-responders) or succeeded (high-responders) in attaining exercise-derived clinically meaningful reductions in body weight (BW) and fat mass (FM) (i.e., 6% for FM and 3% for BW) was assessed.

Methods
Individuals with T2DM (n = 80) were enrolled in a RCT with three groups: resistance training combined with moderate-intensity continuous training (MICT) or high-intensity interval training (HIIT) and a control group. Of the 80 participants, 56 (completed data) were considered for this secondary analysis. NEPA and estimated NEAT were obtained by accelerometry and body composition through dual-energy X-ray absorptiometry.

Results
After adjustments, no time*group interactions were found for estimated NEAT in the MICT (β = − 5.33, p = 0.366) and HIIT (β = − 5.70, p = 0.283), as well as for NEPA in the MICT (β = − 452.83, p = 0.833) and HIIT (β = − 2770.76, p = 0.201), when compared to controls. No compensatory changes in NEPA and estimated NEAT were observed when considering both low-responders and high-responders to FM and BW when compared to controls.

Conclusions
Both MICT and HIIT did not result in any compensatory changes in estimated NEAT and NEPA with the intervention on non-training days. Moreover, no changes in estimated NEAT and NEPA were found when categorizing our participants as low-responders and high-responders to FM and BW when compared to controls.
Trial registration clinicaltrials.gov ID.
NCT03144505.
Sensor integrating plain bearings: design of an energy-autonomous, temperature-based condition monitoring system
Authors: Baszenski Thao , Kauth Kevin , Kratz Karl-Heinz , Gutiérrez Guzmán Francisco , Jacobs Georg , Gemmeke Tobias 
Published: February 2023

AbstractPlain bearings are increasingly being used under mixed friction, resulting in a higher risk of wear-induced failure. Therefore, the relevance of condition monitoring is increasing, which can enable the early detection of wear inducing conditions. The presented work focuses on the development of an energy-autonomous, temperature-based condition monitoring system for plain bearings. The components of the condition monitoring system are to be integrated directly into the machine element and operating data to be transmitted wirelessly.The fundamental monitoring approach pursued, is the in-operando calculation of the lubrication gap height via the standardized Gümbel-curve based on the temperature map in the plain bearing. If the gap height undercuts a critical threshold, the plain bearing will operate in mixed friction. Thus, representing a potentially wear-causing operating point. The energy required for operating the system is harvested thermoelectrically within the plain bearing via thermo-generators, which use the temperature gradients in the plain bearing during operation.In this work, the simulative evaluation of the harvestable energy will be presented. The usable temperature difference will be determined for varying positioning of the energy harvester. The results obtained allow the subsequent manufacture of a first Sensor integrating Machine Element (SiME)-prototype. In this context a feasible setup regarding the types and number of sensors as well as a microcontroller for processing the measured data will be defined with respect to the harvestable energy. Furthermore, the principle for temperature-based gap height determination will be introduced, applied for different operating points and compared to simulative results.
The Western origins of mindfulness therapy in ancient Rome
Authors: Cavanna Andrea E. , Purpura Giulia , Riva Anna , Nacinovich Renata , Seri Stefano 
Published: February 2023

AbstractStoic philosophy has multiple parallels with cognitive behavioural therapy interventions. In their ancient texts, the Roman Stoics present a set of theoretical principles and behavioural strategies that are directly relevant to the clinical care of patients with a wide range of neuropsychiatric conditions. Mindfulness is a key component of the ‘third wave’ of modern psychotherapy that closely resembles the ancient Stoic practice of attention or ‘concentration on the present moment’. Stoic mindfulness draws attention to one of the main principles driving both Stoicism and modern psychotherapy: the assumption that cognitive activity (reasoning) mediates emotions and behaviours. This principle can be traced back to Epictetus’ Enchiridion, where he recognises that ‘men are disturbed not by things, but by the views which they take of things’. It has been shown that cognitive behavioural therapies and mindfulness-based interventions directed at patients with neuropsychiatric disorders were originally developed as Stoic-inspired treatment interventions. Both Albert Ellis and Aaron Beck (the founders of rational emotive behaviour therapy and cognitive behavioural therapy, respectively) explicitly acknowledged the role of Stoicism as the philosophical precursor of their treatment approaches. The effective implementation of evidence-based guidelines would benefit from an increased awareness of the influence of the Stoic tradition of philosophical therapy on the treatment approaches currently in use in neuropsychiatry.