bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rational Design of Tunable Near‐Infrared Oxazine Probe with Large Stokes Shift for Leucine Aminopeptidase Detection and Imaging
Authors: Li Haiyan , Shen Yang , Dong Zhengkun , Li Wei , Yuan Lin 
Published: September 2023

AbstractNear‐Infrared (NIR) fluorescence imaging with the advantages of deep tissue penetration and minimum background, has been widely employed and developed in the study of biological applications. However, small Stokes shifts, difficulty in optical tuning, and pH sensitivity are still the major limitations faced by current NIR dyes. To solve these problems, we rationally designed a pH insensitive amino‐tunable NIR oxazine fluorophore DQF‐NH2, which exhibited large Stokes shift (125 nm) accompanied with NIR excitation/emission due to the introduction an asymmetrical alternating vibronic feature. By benefiting from the excellent photophysical properties of DQF‐NH2, we have successfully constructed the probe DQF‐NH2‐LAP with the ability to detect endogenous LAP. Bioimaging assays demonstrated that DQF‐NH2‐LAP can not only effectively detect LAP in living cells, but also was successfully applied to image tumor tissue in vivo. We anticipate that the functionalizable dye DQF‐NH2 may be a potential new NIR dye platform with an optically tunable group for the development of future desirable probes for bioimaging.
CEO poverty experience and corporate environmental violations
Authors: Du Lei , Ren Shenggang 
Published: September 2023

AbstractThis study explores the effect of chief executive officer (CEO) early‐life poverty experiences on firms' environmental violations. By integrating upper echelon theory and imprinting theory, we argue that CEOs' poverty imprints cause them to re‐evaluate their life priorities and channel managerial attention to their firms' economic goal domains, thereby encouraging CEOs to pursue firm economic performance rather than environmental responsibility. Consequently, they manifest a tendency to engage in environmental violations. An analysis of a large sample of publicly listed Chinese firms during the period 2008–2020 provides strong support for the positive effect of CEO poverty experience on corporate environmental violations. This poverty imprint effect is weaker for CEOs with higher education backgrounds but stronger for firms with greater horizontal performance gaps. Our results survived an endogeneity check and various robustness tests.
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates
Authors: Tsukamoto Keisuke , Hamamoto Ryo , Oguri Ryota , Miura Aki , Iwasaki Takuma , Sukegawa Takeshi 
Published: September 2023

AbstractFouling by protein aggregates reduces virus removal filter performance. In the present study, we investigated the effects of different‐sized protein aggregates on fouling and aggregate retention in order to better understand the fouling mechanisms. Human immunoglobulin G was denatured by heating to produce aggregates of various sizes and then fractionated by size exclusion chromatography into different‐sized aggregates with a narrow size distribution. The fractionated aggregates were filtered on Planova 20N, a virus removal filter known for its stable filtration capability. Analysis of flux behavior demonstrated different flux decrease patterns for different‐sized aggregates. Observation of aggregate retention by staining revealed that larger aggregates were captured closer to the inner surface of the membrane while smaller aggregates penetrated farther into the membrane. These findings demonstrate that Planova 20N has a gradient structure with decreasing pore size from the inner to the outer surface of the membrane. This structure minimizes fouling and enables stable filtration by protecting the smaller pores located closer to the outer surface from clogging by large aggregates. Applying the predominant clogging models to the present filtrations revealed that clogging behavior transitioned from complete blocking to cake filtration as filtration progressed. In this combination model, after a certain number of pores are blocked by complete blocking, newly arrived aggregates begin to accumulate on previously captured aggregates, generating cake between capture layers within the membrane. Application of the approaches described here will facilitate elucidation of membrane fouling and virus removal mechanisms.
One‐step production of fully biotinylated and glycosylated human Fc gamma receptors
Authors: Kang Minhyo , Wang Zening , Ge Xin 
Published: September 2023

AbstractInitiating and regulating humoral immunity, Fc gamma receptors (FcγRs) have been identified both as therapeutics and as drug targets, and thus production of biologically active FcγRs is highly demanded for biopharmaceutical development. Focusing on low‐affinity FcγRs IIA (131H/R allotypes), IIB, and IIIA (176F/V), this study used human 293‐F cells to achieve correct post‐translational modifications (PTMs) including biotinylation, N‐glycosylation, and disulfides. Approaches involving co‐expression of FcγR‐AviTag and Escherichia coli biotin ligase BirA, endoplasmic reticulum retention, stable and transient transfections, and optimization of transgene ratio were investigated. Protein electrophoresis under reducing and non‐reducing conditions, enzymatic deglycosylation, streptavidin pull‐down assays, and binding kinetic analysis collectively indicated that the produced FcγR ectodomains were fully biotinylated, N‐glycosylated, had formed disulfide bond, and exhibited expected binding affinities toward IgG1 trastuzumab and its Fc mutants. A clear trade‐off between production yield and PTM quality was also observed. Achieving multiple types of PTMs completely by one‐step cell culture should have applications for the production of a variety of complex proteins of biomedical importance.
Activation of PI3K/Akt pathway by G protein‐coupled receptor 37 promotes resistance to cisplatin‐induced apoptosis in non‐small cell lung cancer
Authors: Liu Han , Zhu Yingjie , Niu Huikun , Jie Jing , Hua Shucheng , Bai Xiaoxue , Wang Shuai , Song Lei 
Published: September 2023

AbstractObjectivesLung cancer is a major public health concern and represents the most common cause of cancer‐related death worldwide. Among eukaryotes, the G protein‐coupled receptor (GPCR) family stands as the largest group of membrane proteins. Alterations in GPCR gene expression and dysregulation of signal transduction have been recognized as the markers of malignancy. As a member of the GPCR family, G protein‐coupled receptor 37 (GPR37) exhibits unknown functions in tumors, particularly in non‐small‐cell lung cancer (NSCLC)MethodsWe explored the expression and prognosis of GPR37 in NSCLC through TCGA, GTEx, GEO, and GEPIA2. We detected the expression of GPR37 in NSCLC tissues and cell lines. The study explored the influence of GPR37 on tumor cell proliferation. Furthermore, we examined the effects of GPR37 on tumor cell apoptosis and invasion. Most importantly, we investigated whether GPR37 affects cisplatin‐induced drug resistance in NSCLC. Furthermore, by conducting animal experiments, we assessed the impact of GPR37 on NSCLC and delved into underlying mechanisms.Results(1) In NSCLC, the expression of GPR37 is markedly higher than that in corresponding normal tissues. We found that elevated GPR37 expression predicts an unfavorable prognosis. (2) It was demonstrated that GPR37 positively regulates NSCLC cell invasion, migration, and proliferation, suppresses cell apoptosis, heightens resistance to cisplatin, and promotes tumor formation and growth. Conversely, we observed that GPR37 knockdown suppresses NSCLC cell invasion, migration, and proliferation, promotes cell apoptosis, increases sensitivity to cisplatin, and affects tumor formation and growth. (3) GPR37 activates PI3K/Akt/mTOR signal transduction pathways to mediate epithelial‐mesenchymal transition (EMT), thereby promoting the progression of NSCLC.ConclusionsIt was suggested that GPR37 acts a crucial role in promoting the occurrence and development of NSCLC. Knockdown of GPR37 significantly inhibits the occurrence and development of NSCLC. Therefore, our findings demonstrated that GPR37 may represent a viable therapeutic target for NSCLC.
Life‐threatening pontine hemorrhage secondary to HELLP syndrome
Authors: Ali Sajjad , Fazal Sara Anjum , Monawwer Syed Abdullah , Anan Mahfuza 
Published: September 2023

Key Clinical MessageHELLP syndrome is a severe form of pre‐eclampsia that typically develops prior to delivery but can sometimes happen postpartum. The classic triad of HELLP syndrome comprises of hemolysis, elevated liver enzyme levels, and low platelet counts. This condition is known to have a higher incidence of fatal neurological complications, such as pontine hemorrhage, when compared to a typical pre‐eclampsia.
An autopsy case of disseminated carcinomatosis of the bone marrow from esophageal adenocarcinoma
Authors: Suzuki Daisuke , Meguro Shiori , Furusawa Shin , Hashimoto Nanako , Kawasaki Hideya , Kosugi Isao , Enomoto Yasunori , Fujihiro Mayu , Tsukui Hiroe , Iwashita Toshihide 
Published: September 2023

Key Clinical MessageDisseminated carcinomatosis of the bone marrow is rare. We present such a case, which is useful for raising awareness about the importance of early diagnosis and treatment of carcinomas complicated by disseminated carcinomatosis of the bone marrow.AbstractThis is the first autopsy report of disseminated carcinomatosis of the bone marrow (DCBM) in esophageal adenocarcinoma. Advanced poorly differentiated adenocarcinoma with signet ring cell carcinoma arising in Barrett's esophagus caused disseminated intravascular coagulation (DIC) with extensive bone marrow metastasis, resulting in death from cerebral hemorrhage. Although DCBM due to malignancy is rare with poor prognosis, it should be considered in malignancies associated with DIC, and prompt initiation of chemotherapy is the only way to improve the patient's prognosis.
Enhanced Catalytic Selectivity in Hydrogenation of Substituted Nitroarenes through Hydrogen Spillover over Sodalite Zeolite Encapsulated Platinum Clusters
Authors: Yang Ganjun , He Yanan , Zhang Chengxi , Sun Zongyu , Han Mengxi , Peng Pai , Yue Yaning , Liu Hongxia , Liu Lei , Chen Junwen , Bai Licheng , Chen Qiang 
Published: September 2023

AbstractHerein, we reported that the platinum (Pt) clusters encapsulated into sodalite (SOD) zeolite as catalyst exhibited 100 % selectivity at 100 % conversion in the selective hydrogenation of p‐chloronitrobenzene to p‐chloroaniline via hydrogen spillover. The direct interaction between p‐chloronitrobenzene and encapsulated Pt was prevented by the shape selectivity of SOD zeolite, reactants were thereby adsorbed onto zeolite outer surface to facilitate the hydrogenation proceeding by hydrogen spillover process. The further kinetic study and DFT calculation showed the excellent catalytic selectivity was ascribed to the much faster rate of hydrogenation of adsorbed nitro group than that of adsorbed C−Cl group.
Principal balances of compositional data for regression and classification using partial least squares
Authors: Nesrstová V. , Wilms I. , Palarea‐Albaladejo J. , Filzmoser P. , Martín‐Fernández J. A. , Friedecký D. , Hron K. 
Published: September 2023

AbstractHigh‐dimensional compositional data are commonplace in the modern omics sciences, among others. Analysis of compositional data requires the proper choice of a log‐ratio coordinate representation, since their relative nature is not compatible with the direct use of standard statistical methods. Principal balances, a particular class of orthonormal log‐ratio coordinates, are well suited to this context as they are constructed so that the first few coordinates capture most of the compositional variability of data set. Focusing on regression and classification problems in high dimensions, we propose a novel partial least squares (PLS) procedure to construct principal balances that maximize the explained variability of the response variable and notably ease interpretability when compared to the ordinary PLS formulation. The proposed PLS principal balance approach can be understood as a generalized version of common log‐contrast models since, instead of just one, multiple orthonormal log‐contrasts are estimated simultaneously. We demonstrate the performance of the proposed method using both simulated and empirical data sets.
Development of the vertebra and fin skeleton in the lamprey and its implications for the homology of vertebrate vertebrae
Authors: Kariyayama Hirofumi , Gogoleva Natalia , Harada Keishi , Yokoyama Hiromasa , Ono Hiroki , Suzuki Daichi G. , Yamazaki Yuji , Wada Hiroshi 
Published: September 2023

AbstractBackgroundAlthough vertebrae are the defining character of vertebrates, they are found only in rudimentary form in extant agnathans. In addition, the vertebrae of agnathans possess several unique features, such as elastin‐like molecules as the main matrix component and late (post‐metamorphosis) differentiation of lamprey vertebrae. In this study, by tracing the developmental process of vertebrae in lamprey, we examined the homology of vertebrae between lampreys and gnathostomes.ResultsWe found that the lamprey somite is first subdivided mediolaterally, with myotome cells differentiating medially and non‐myotome cells emerging laterally. Subsequently, collagen‐positive non‐myotome cells surround the myotome. This pattern of somitogenesis is rather similar to that in amphioxi and sheds doubt on the presence of a sclerotome, in terms of mesenchyme cells induced by a signal from the notochord, in lamprey. Further tracing of non‐myotome cell development revealed that fin cartilage develops in ammocoete larvae approximately 35 mm in body length. The development of the fin cartilage occurs much earlier than that of the vertebra whose development proceeds during metamorphosis.ConclusionWe propose that the homology of vertebrae between agnathans and gnathostomes should be discussed carefully, because the developmental process of the lamprey vertebra is different from that of gnathostomes.
Comparative outcomes between transvaginal endoscopic hysterectomy and total laparoscopic hysterectomy in patients with benign uterine disease: A single‐center, retrospective, cohort, interrupted time‐series study
Authors: Jiamset Ingporn , Uttraporn Pawara , Suphasynth Yuthasak 
Published: September 2023

AbstractObjectivesTo compare the surgical outcomes of total laparoscopic hysterectomy (TLH) and transvaginal endoscopic hysterectomy (TVEH) for benign uterine diseases.MethodsThis retrospective, cohort, interrupted time‐series study included patients who underwent TLH between January 2013 and September 2016 and TVEH between October 2016 and June 2020. Median difference regression was used to compare the hospital length of stay (LOS) and operative time between the groups. Risk difference regression was used to analyze the proportion of patients who developed postoperative fever or vaginal stump infection.ResultsOf the total 171 patients enrolled, 101 and 70 underwent TVEH and TLH, respectively. The mean ages of patients in the TVEH and TLH groups were 46.49 and 46.17 years, respectively. No conversion was observed. Ureteric injury occurred in one patient in the TVEH group, whereas there was no organ injury in any of the patients in the TLH group. Compared with those in the TLH group, patients in the TVEH group had a significantly shorter median operative time, shorter hospital LOS, lower morphine use, and lower postoperative febrile morbidity rates. However, no significant difference was observed in the rate of vaginal stump infection between the groups.ConclusionGiven the shorter operative time, shorter hospital LOS, less febrile morbidity, and lower morphine use in patients with TVEH than in those with TLH, TVEH should be considered as an alternative hysterectomy procedure for benign uterine diseases.
Durable response to pembrolizumab in microsatellite instability‐high advanced adrenocortical carcinoma
Authors: Senda Masaya , Hashimoto Kohei , Shindo Tetsuya , Kobayashi Ko , Tanaka Toshiaki , Masumori Naoya 
Published: September 2023

IntroductionAdvanced adrenocortical carcinoma has a poor prognosis and is treated with chemotherapy that includes mitotane with etoposide, doxorubicin, and cisplatin as first‐line therapy. However, second‐line therapy has not been determined yet. Pembrolizumab has been approved for high microsatellite instability for which standard treatments have failed.Case presentationHere, we present a patient with advanced adrenocortical carcinoma treated with complete surgical resection. 21 months later, he had local and metastatic recurrences. After four cycles of first‐line therapy, we switched to pembrolizumab because microsatellite instability‐high was detected in his tumor. He has received mitotane and pembrolizumab for 15 months, and this has exerted a radiographical response without severe adverse events.ConclusionWe presented a patient with microsatellite instability‐high advanced adrenocortical carcinoma treated with pembrolizumab and mitotane.
Water requirements of banana and papaya under different water table depths and soil types in the Lower Indus Basin of Pakistan
Authors: Gul Nazar , Salam Hafiz Abdul , Ashraf Muhammad 
Published: September 2023

AbstractThe water requirements of banana and papaya, the two most important fruit crops of Pakistan, are not known because farmers continue to apply water even if the crop does not need it. In this study, the evapotranspiration (ET), groundwater contribution (GW) and crop coefficient (Kc) values of banana (1995–1999) and papaya (2013–2016) were determined under different water table depths (WTDs) and soil types using drainage‐type lysimeters. The WTDs were maintained at 1.50, 2.00 and 2.50 m from the ground surface by means of Mariotte bottles. The banana ET during the first year was between 1653 and 1992 mm with a GW contribution of 11–20%, whereas during the second to fifth years, it was between 2430 and 3023 mm with a GW contribution of 10–16%. The banana yield was in the range of 58,638–79,780 kg ha−1, and the water use efficiency (WUE) was between 2.65 and 3.31 kg m−3. The papaya ET was between 1478 and 1766 mm with a GW contribution of 7–18% at 1.50–2.50 m WTDs. The papaya yield was in the range of 61,184–83,004 kg ha−1 with a WUE of 4.13–4.70 kg m−3. Therefore, GW contribution is an important component of the water balance under shallow water table conditions and should be considered when devising irrigation scheduling for banana and papaya crops.
CBX8 promotes lung adenocarcinoma growth and metastasis through transcriptional repression of CDKN2C and SCEL
Authors: Chen Hao , Su Yijie , Yang Lihong , Xi Lishan , Li Xuanyuan , Lan Bei , Liu Min , Xuan Chenghao 
Published: September 2023

AbstractDysregulation of polycomb group (PcG) proteins that mediate epigenetic gene silencing contributes to tumorigenesis. As core components of the polycomb repressive complex 1 (PRC1), chromobox (CBX) proteins recognize H3K27me3 to recruit PRC1 to maintain a repressive transcriptional state. However, the individual biological functions of these CBX proteins in tumorigenesis warrant in‐depth investigation. In this study, we analyzed the mRNA expression of CBX family genes across multiple cancers using The Cancer Genome Atlas data and found different expression patterns of the five CBX genes in different types of cancer. This analyses together with the result of immunohistochemistry indicated that CBX8 expression was significantly higher in lung adenocarcinoma (LUAD) tissues compared to adjacent nontumor tissues. Overexpression approaches demonstrated that CBX8 facilitated LUAD cell proliferation and migration in vitro. Consistently, CBX8 knockdown reduced LUAD cell proliferation and migration in both cell culture and mouse models. RNA sequencing combined with real‐time RT‐PCR assays revealed CDKN2C and SCEL as target genes of CBX8. Furthermore, chromatin immunoprecipitation assays indicated that CBX8 directly bound to the promoters of CDKN2C and SCEL to establish H2AK119ub. CBX8 depletion reduced the enrichment of H2AK119ub on CDKN2C and SCEL promoters. Moreover, depletion of CDKN2C and SCEL restored the repressed growth and invasion ability of LUAD cells caused by CBX8 knockdown. These findings demonstrate that CBX8 promotes LUAD growth and metastasis through the transcriptional repression of CDKN2C and SCEL. Our study uncovers the oncogenic role of CBX8 in LUAD progression and provides a new target for the diagnosis and therapy of LUAD.
Validity of computed tomography defined body composition as a prognostic factor for functional outcome after kidney transplantation
Authors: Swaab Tim D. A. , Quint Evelien E. , Westenberg Lisa B. , Zorgdrager Marcel , Segev Dorry L. , McAdams‐DeMarco Mara A. , Bakker Stephan J. L. , Viddeleer Alain R. , Pol Robert A. 
Published: September 2023

AbstractBackgroundThe prevalence of sarcopenia is markedly higher in kidney transplant candidates than in the general population. It is a syndrome characterized by progressive and generalized loss of skeletal muscle mass and strength, which increases the risk of adverse postoperative outcomes.MethodsWe studied the impact of computed tomography defined preoperative sarcopenia, defined as a skeletal muscle index below age and gender specific cut‐off values, on postoperative physical functional outcomes (grip strength, 4‐m walking test, timed up and go, and sit to stand) at 6 months follow up.ResultsA total of 107 patients transplanted between 2015 and 2019 were included in this single‐centre study. Mean age was 60.3 (±13.1), and 68.2% of patients were male. Ten patients (9.4%) were identified as sarcopenic. Sarcopenic patients were younger (55.6 (±15.1) vs. 60.8 (±12.9) years), more likely to be female (60.0% vs. 28.9%), and had an increased dialysis vintage (19 [2.5–32.8] vs. 9 [0.0–21.0] months) in comparison with their non‐sarcopenic counterparts. In univariate analysis, they had a significantly lower body mass index and skeletal muscle area (P ≤ 0.001). In multivariate regression analysis, skeletal muscle index was significantly associated with grip strength (β = 0.690, R2 = 0.232) and timed up and go performance (β = −0.070, R2 = 0.154).ConclusionsWe identified a significant association between sarcopenia existing pre‐transplantation and poorer 6 months post‐transplantation physical functioning with respect to hand grip strength and timed up and go tests in kidney transplant recipients. These results could be used to preoperatively identify patients with an increased risk of poor postoperative physical functional outcome, allowing for preoperative interventions to mitigate these risks.