bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caracterización del perfil profesional y rol del fisioterapeuta del área deportiva en Cauca y Nariño.
Authors: Muñoz Luna Jonathan Adrián , Portilla  Matacea Yuleny Lucero , Álvarez Pantoja Sara Juliana 
Published: January 2023

Introducción: El rol del fisioterapeuta deportivo está definido respecto a las competencias que debería ejercer, pero no hay claridad en que si se está cumpliendo con dichas competencias debido a las escasas investigaciones. Objetivo: Describir el perfil profesional y el rol del fisioterapeuta deportivo en el Cauca y Nariño. Método: Tipo de estudio descriptivo – trasversal, la muestra del estudio fueron 21 profesionales de Fisioterapia vinculados al área de deporte, en los departamentos de Cauca y Nariño en el 2019. Resultados: el género masculino prevalece, en donde más laboran los fisioterapeutas es en institutos o secretarías de deporte, en nivel de formación académica 52,4% tenía posgrado en deporte y actividad física, en el tipo de contratación tiene mayor prevalencia la prestación de servicios. De las competencias de mayor actuación prevalece la prevención, el retorno al deporte, promoción de la salud, el entrenamiento. En las funciones del Ft, se reporta en mayor medida él siempre está presente en los entrenos, él nunca está presente en las competencias y a veces viaja con el equipo, el 85,7% reporto que si realizan prevención de lesiones, en cuanto a la indicación de Fisioterapia el 42,9% reporto que se realiza por médico y fisioterapeuta. Conclusiones: La mitad de los fisioterapeutas deportivos realizan estudios de postgrado. El rol del fisioterapeuta es principalmente para la rehabilitación de lesiones. La mayoría de fisioterapeutas no basan su abordaje en modelos teóricos o en evidencia científica; La indicación de FT deportiva en su mayoría es realizada en conjunto Fisioterapeuta- Medico.
Prevalencia de las patologías musculoesqueléticas en Gamers
Authors: Reyes Duarte María Tatiana , González Daza Paula Yineth 
Published: January 2023

En la actualidad las alteraciones musculoesqueléticas en jugadores de videojuegos son mucho más evidentes, teniendo en cuenta que hay aproximadamente 2,800 millones de gamers en el mundo y el 38% de ellos juegan videojuegos en un ordenador, consola u otro dispositivo electrónico. Lo que hace evidente la necesidad de ver, que repercusión en la salud puede tener esta práctica, por lo que se pretende realizar una búsqueda exhaustiva sobre artículos en diferentes bases de datos, enfocado en las alteraciones musculoesqueléticas en jugadores aficionados. De acuerdo con esto tras una revisión de diferentes bases de datos como pubmed, Scielo, Science Direct, en Inglés y español, de los últimos 5 años, Los resultados demostraron que la evidencia reporta un 80% de alteraciones y molestias en miembros superiores y el 10% presentó una patología en este caso Síndrome del Túnel Carpiano, debido a que hay poca información sobre las posibles y más alteraciones en los jugadores, lo que hace evidente la necesidad de crear métodos de análisis y evaluación a nivel fisioterapéutico conociendo y realizando una batería de evaluación muy detallada en los jugadores.
A score of bioavailable strontium isotope archaeology in China: Retrospective and prospective
Authors: Tang Zihua , Wang Xueye 
Published: January 2023

Bioavailable strontium isotope analysis was proposed for prehistoric human ecology almost 40 years ago and rapidly became one of the most important tools to trace past migratory behaviours. Since its first introduction to China in 2003, this method has greatly improved our understanding of migrations on individual- and community-scales over the past 20 years. This paper summarizes the current state of knowledge regarding identifying non-locals, utilizing animal resources, and strontium isoscapes for China based on empirical data. By incorporating additional baseline data, we updated and extended the current bioavailable strontium isoscape for China and Southeast and South Asia. These data will shed new light on intercultural communications and the spread of customs and technologies. In the future, strontium isotope analysis will involve an integrated provenancing system along with multiple approaches such as various isotopes and different modellings. Correspondingly, the existing isoscape needs to improve its spatial resolution and predictive accuracy to source the non-local archaeological biological remains. Furthermore, advances in analytical techniques allow us to reconstruct lifetime mobility of animals and humans at high temporal resolution.
A novel clinical−radiomic nomogram for the crescent status in IgA nephropathy
Authors: Qin Xiachuan , Xia Linlin , Hu Xiaomin , Xiao Weihan , Huaming Xian , Xisheng Xie , Zhang Chaoxue 
Published: January 2023

ObjectiveWe used machine-learning (ML) models based on ultrasound radiomics to construct a nomogram for noninvasive evaluation of the crescent status in immunoglobulin A (IgA) nephropathy.MethodsPatients with IgA nephropathy diagnosed by renal biopsy (n=567) were divided into training (n=398) and test cohorts (n=169). Ultrasound radiomic features were extracted from ultrasound images. After selecting the most significant features using univariate analysis and the least absolute shrinkage and selection operator algorithm, three ML algorithms were assessed for final radiomic model establishment. Next, clinical, ultrasound radiomic, and combined clinical−radiomic models were compared for their ability to detect IgA crescents. The diagnostic performance of the three models was evaluated using receiver operating characteristic curve analysis.ResultsThe average area under the curve (AUC) of the three ML radiomic models was 0.762. The logistic regression model performed best, with AUC values in the training and test cohorts of 0.838 and 0.81, respectively. Among the final models, the combined model based on clinical characteristics and the Rad score showed good discrimination, with AUC values in the training and test cohorts of 0.883 and 0.862, respectively. The decision curve analysis verified the clinical practicability of the combined nomogram.ConclusionML classifier based on ultrasound radiomics has a potential value for noninvasive diagnosis of IgA nephropathy with or without crescents. The nomogram constructed by combining ultrasound radiomic and clinical features can provide clinicians with more comprehensive and personalized image information, which is of great significance for selecting treatment strategies.
Shared sex hormone metabolism-related gene prognostic index between breast and endometrial cancers
Authors: Duan Junyi , Liu Chenan , Yi Jiahong , Wang Yun 
Published: January 2023

AimsAs sex hormone-dependent tumors, it remains to be clarified whether there is a common genetic signature and its value between breast and endometrial cancers. The aim of this study was to establish the shared sex hormone metabolism-related gene prognostic index (SHMRGPI) between breast and endometrial cancers and to analyze its potential role in the therapeutic and prognostic assessment of endometrial cancers.MethodsUsing transcriptome data from TCGA, tumor-associated gene modules were identified by weighted gene co-expression network analysis, and the intersection of module genes with female sex hormone synthesis and metabolism genes was defined as sex hormone metabolism-related gene. SHMRGPI was established by the least absolute shrinkage and selection operator and Cox regression. Its prognostic value of patients with endometrial cancer was validated, and a nomogram was constructed. We further investigated the relationship between SHMRGPI groups and clinicopathological features, immune infiltration, tumor mutation burden, and drug sensitivity.ResultsA total of 8 sex hormone metabolism-related gene were identified as key genes for the construction of prognostic models. Based on SHMRGPI, endometrial cancer patients were divided into high and low SHMRGPI groups. Patients in the low SHMRGPI group had longer overall survival (OS) compared with the high group (P&lt; 0.05). Furthermore, we revealed significant differences between SHMRGPI groups as regards tumor immune cell infiltration, somatic mutation, microsatellite instability and drug sensitivity. Patients with low SHMRGPI may be the beneficiaries of immunotherapy and targeted therapy.ConclusionsThe SHMRGPI established in this study has prognostic power and may be used to screen patients with endometrial cancer who may benefit from immunotherapy or targeted therapy.
Long lead-time radar rainfall nowcasting method incorporating atmospheric conditions using long short-term memory networks
Authors: Zhu Kexin , Yang Qiqi , Zhang Shuliang , Jiang Shuai , Wang Tianle , Liu Jinchen , Ye Yuxuan 
Published: January 2023

High-resolution radar rainfall data have great potential for rainfall predictions up to 6 h ahead (nowcasting); however, conventional extrapolation approaches based on in-built physical assumptions yield poor performance at longer lead times (3–6 h), which limits their operational utility. Moreover, atmospheric factors in radar estimate errors are often ignored. This study proposed a radar rainfall nowcasting method that attempts to achieve accurate nowcasting of 6 h using long short-term memory (LSTM) networks. Atmospheric conditions were considered to reduce radar estimate errors. To build radar nowcasting models based on LSTM networks (LSTM-RN), approximately 11 years of radar, gauge rainfall, and atmospheric data from the UK were obtained. Compared with the models built on optical flow (OF-RN) and random forest (RF-RN), LSTM-RN had the lowest root-mean-square errors (RMSE), highest correlation coefficients (COR), and mean bias errors closest to 0. Furthermore, LSTM-RN showed a growing advantage at longer lead times, with the RMSE decreasing by 17.99% and 7.17% compared with that of OF-RN and RF-RN, respectively. The results also revealed a strong relationship between LSTM-RN performance and weather conditions. This study provides an effective solution for nowcasting radar rainfall at long lead times, which enhances the forecast value and supports practical utility.
On-site and visual detection of sorghum mosaic virus and rice stripe mosaic virus based on reverse transcription-recombinase-aided amplification and CRISPR/Cas12a
Authors: Wang Junkai , Huang Xiuqin , Chen Siping , Chen Jiahao , Liang Zhengyi , Chen Biao , Yang Xin , Zhou Guohui , Zhang Tong 
Published: January 2023

Rapid, sensitive and visual detection of plant viruses is conducive to effective prevention and control of plant viral diseases. Therefore, combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR/Cas12a-based visual nucleic acid detection system targeting sorghum mosaic virus and rice stripe mosaic virus, which cause harm to crop production in field. When the RT-RAA products were recognized by crRNA and formed a complex with LbCas12a, the ssDNA labeled with a quenched green fluorescent molecule will be cleaved by LbCas12a, and then a significant green fluorescence signal will appear. The entire detection process can be completed within 30 min without using any sophisticated equipment and instruments. The detection system could detect samples at a dilution of 107, about 104-fold improvement over RT-PCR, so the system was successfully to detect rice stripe mosaic virus in a single leafhopper, which is the transmission vector of the virus. Finally, the CRISPR/Cas12a-based detection system was utilized to on-site detect the two viruses in the field, and the results were fully consistent with that we obtained by RT-PCR in laboratory, demonstrating that it has the application prospect of detecting important crop viruses in the field.
Comparisons of short-term and long-term results between laparoscopic between open pancreaticoduodenectomy for pancreatic tumors: A systematic review and meta-analysis
Authors: Qiu Hongquan , Zhang Liang , Wang Dongzhi , Miao Haiyan , Zhang Yu 
Published: January 2023

Objective: The efficacy of pancreaticoduodenectomy and open pancreaticoduodenectomy for pancreatic tumors is controversial. The study aims to compare the efficacy of laparoscopic pancreaticoduodenectomy (LPD) and open pancreaticoduodenectomy (OPD) in the treatment of pancreatic tumors through systematic evaluation and meta-analysis.Methods: PubMed, Embase, Cochrane Library and Web of science databases were searched for clinical studies on the treatment of pancreatic tumors with LPD and OPD. The end time for the searches was 20 July 2022. Rigorous inclusion and exclusion criteria were used to screen the articles, the Cochrane manual was used to evaluate the quality of the included articles, and the stata15.0 software was used for statistical analysis of the indicators.Results: In total, 16 articles were included, including two randomized controlled trials and 14 retrospective studies. Involving a total of 4416 patients, 1275 patients were included in the LPD group and 3141 patients in the OPD group. The results of the meta-analysis showed that: the operation time of LPD was longer than that of OPD [WMD = 56.14,95% CI (38.39,73.89), p = 0.001]; the amount of intraoperative blood loss of LPD was less than that of OPD [WMD = −120.82,95% CI (−169.33, −72.30), p = 0.001]. No significant difference was observed between LPD and OPD regarding hospitalization time [WMD = −0.5,95% CI (−1.35, 0.35), p = 0.250]. No significant difference was observed regarding postoperative complications [RR = 0.96,95% CI (0.86,1.07, p = 0.463]. And there was no significant difference regarding 1-year OS and 3-year OS: 1-year OS [RR = 1.02,95% CI (0.97,1.08), p = 0.417], 3-year OS [RR = 1.10 95% CI (0.75, 1.62), p = 0.614%].Conclusion: In comparison with OPD, LPD leads to less blood loss but longer operation time, therefore the bleeding rate per unit time of LPD is less than that of OPD. LPD has obvious advantages. With the increase of clinical application of LPD, the usage of LPD in patients with pancreatic cancer has very good prospect. Due to the limitations of this paper, in future studies, more attention should be paid to high-quality, multi-center, randomized controlled studies.
Transcriptome and metabolome profiling of the medicinal plant Veratrum mengtzeanum reveal key components of the alkaloid biosynthesis
Authors: Liu Jiajia , Han Lijun , Li Guodong , Zhang Aili , Liu Xiaoli , Zhao Mingzhi 
Published: January 2023

Veratrum mengtzeanum is the main ingredient for Chinese folk medicine known as “Pimacao” due to its unique alkaloids. A diverse class of plant-specific metabolites having key pharmacological activities. There are limited studies on alkaloid synthesis and its metabolic pathways in plants. To elucidate the alkaloid pathway and identify novel biosynthetic enzymes and compounds in V. mengtzeanum, transcriptome and metabolome profiling has been conducted in leaves and roots. The transcriptome of V. mengtzeanum leaves and roots yielded 190,161 unigenes, of which 33,942 genes expressed differentially (DEGs) in both tissues. Three enriched regulatory pathways (isoquinoline alkaloid biosynthesis, indole alkaloid biosynthesis and tropane, piperidine and pyridine alkaloid biosynthesis) and a considerable number of genes such as AED3-like, A4U43, 21 kDa protein-like, 3-O-glycotransferase 2-like, AtDIR19, MST4, CASP-like protein 1D1 were discovered in association with the biosynthesis of alkaloids in leaves and roots. Some transcription factor families, i.e., AP2/ERF, GRAS, NAC, bHLH, MYB-related, C3H, FARI, WRKY, HB-HD-ZIP, C2H2, and bZIP were also found to have a prominent role in regulating the synthesis of alkaloids and steroidal alkaloids in the leaves and roots of V. mengtzeanum. The metabolome analysis revealed 74 significantly accumulated metabolites, with 55 differentially accumulated in leaves compared to root tissues. Out of 74 metabolites, 18 alkaloids were highly accumulated in the roots. A novel alkaloid compound viz; 3-Vanilloylygadenine was discovered in root samples. Conjoint analysis of transcriptome and metabolome studies has also highlighted potential genes involved in regulation and transport of alkaloid compounds. Here, we have presented a comprehensive metabolic and transcriptome profiling of V. mengtzeanum tissues. In earlier reports, only the roots were reported as a rich source of alkaloid biosynthesis, but the current findings revealed both leaves and roots as significant manufacturing factories for alkaloid biosynthesis.
Alkaline sphingomyelinase (NPP7) impacts the homeostasis of intestinal T lymphocyte populations
Authors: Alyamani Manar , Kadivar Mohammad , Erjefält Jonas , Johansson-Lindbom Bengt , Duan Rui-Dong , Nilsson Åke , Marsal Jan 
Published: January 2023

Background and aimAlkaline sphingomyelinase (NPP7) is expressed by intestinal epithelial cells and is crucial for the digestion of dietary sphingomyelin. NPP7 also inactivates proinflammatory mediators including platelet-activating factor and lysophosphatidylcholine. The aim of this study was to examine a potential role for NPP7 in the homeostasis of the intestinal immune system.MethodsWe quantified the numbers of B-lymphocytes, plasma cells, T-lymphocytes including regulatory T-lymphocytes (Tregs), natural killer cells, dendritic cells, macrophages, and neutrophils, in the small and large intestines, the mesenteric lymph nodes and the spleens of heterozygous and homozygous NPP7 knockout (KO) and wildtype (WT) mice. Tissues were examined by immunohistochemistry and stainings quantified using computerized image analysis.ResultsThe numbers of both small and large intestinal CD3ε+, CD4+, and CD8α+ T-lymphocytes were significantly higher in NPP7 KO compared to WT mice (with a dose-response relationship in the large intestine), whereas Treg numbers were unchanged, and dendritic cell numbers reduced. In contrast, the numbers of CD3ε+ and CD4+ T-lymphocytes in mesenteric lymph nodes were significantly reduced in NPP7 KO mice, while no differences were observed in spleens. The numbers of B-lymphocytes, plasma cells, natural killer cells, macrophages, and neutrophils were similar between genotypes.ConclusionNPP7 contributes to the regulation of dendritic cell and T-lymphocyte numbers in mesenteric lymph nodes and both the small and large intestines, thus playing a role in the homeostasis of gut immunity. Although it is likely that the downstream effects of NPP7 activity involve the sphingomyelin metabolites ceramide and spingosine-1-phosphate, the exact mechanisms behind this regulatory function of NPP7 need to be addressed in future studies.
High levels of kappa free light chain synthesis predict cognitive decline in relapsing-remitting multiple sclerosis
Authors: Rosenstein Igal , Axelsson Markus , Novakova Lenka , Rasch Sofia , Blennow Kaj , Zetterberg Henrik , Lycke Jan 
Published: January 2023

BackgroundEvolving evidence suggests that measurement of cerebrospinal fluid (CSF) kappa free light chain (KFLC) synthesis has high diagnostic sensitivity and specificity for multiple sclerosis (MS), but its prognostic ability is less investigated. The usefulness of KFLC in predicting cognitive impairment (CI) is still unknown.MethodsIn a monocentric longitudinal retrospecitve cohort study, KFLC-index ([CSF KFLC/serum KFLC]/[CSF albumin/serum albumin]) measured by latex-enhanced immunonephelometry was prospectively determined as part of the diagnostic workup in patients with early relapsing-remitting MS (RRMS, n=77). The ability of KFLC-index to predict information processing speed (IPS) worsening as assessed with the symbol digit modalities test (SDMT) was investigated in univariable and multivariable models.ResultsIn patients with KFLC-index&gt;100 (n=31), 11 subjects (35.5%) showed reduced SDMT scores by ≥8 points at follow-up (mean follow-up time 7.3 ± 2.6 years), compared with their baseline scores (p=0.01). Baseline KFLC-index&gt;100 was strongly associated with a higher hazard of SDMT score reduction at follow-up (adjusted hazard ratio 10.5, 95% confidence interval 2.2-50.8, p=0.003; median time to SDMT reduction 7 years).ConclusionIntrathecal KFLC synthesis has become an attractive diagnostic tool for MS. We show for the first time that in a real-world setting of early RRMS, KFLC-index predicted cognitive decline. Whether this predictive ability of KFLC-index also concerns other cognitive domains than IPS, warrants further investigations.
Significance of logistic regression scoring model based on natural killer cell-mediated cytotoxic pathway in the diagnosis of colon cancer
Authors: Ye Zhen , Zhang Huanhuan , Liang Jianwei , Yi Shuying , Zhan Xianquan 
Published: January 2023

BackgroundThe poor clinical accuracy to predict the survival of colon cancer patients is associated with a high incidence rate and a poor 3-year survival rate. This study aimed to identify the poor prognostic biomarkers of colon cancer from natural killer cell-mediated cytotoxic pathway (NKCP), and establish a logistical regression scoring model to predict its prognosis.MethodsBased on the expressions and methylations of NKCP-related genes (NRGs) and the clinical information, dimensionality reduction screening was performed to establish a logistic regression scoring model to predict survival and prognosis. Risk score, clinical stage, and ULBP2 were used to establish a logistic regression scoring model to classify the 3-year survival period and compare with each other. Comparison of survival, tumor mutation burden (TMB), estimation of immune invasion, and prediction of chemotherapeutic drug IC50 were performed between low- and high-risk score groups.ResultsThis study found that ULBP2 was significantly overexpressed in colon cancer tissues and colon cancer cell lines. The logistic regression scoring model was established to include six statistically significant features: S = 1.70 × stage – 9.32 × cg06543087 + 6.19 × cg25848557 + 1.29 × IFNA1 + 0.048 × age + 4.37 × cg21370856 − 8.93, which was used to calculate risk score of each sample. The risk scores, clinical stage, and ULBP2 were classified into three-year survival, the 3-year prediction accuracy based on 10-fold cross-validation was 80.17%, 67.24, and 59.48%, respectively. The survival time of low-risk score group was better than that of the high-risk score group. Moreover, compared to high-risk score group, low-risk score group had lower TMB [2.20/MB (log10) vs. 2.34/MB (log10)], higher infiltration score of M0 macrophages (0.17 vs. 0.14), and lower mean IC50 value of oxaliplatin (3.65 vs 3.78) (p &lt; 0.05).ConclusionsThe significantly upregulated ULBP2 was a poor prognostic biomarker of colon cancer. The risk score based on the six-feature logistic regression model can effectively predict the 3-year survival time. High-risk score group demonstrated a poorer prognosis, higher TMB, lower M0 macrophage infiltration score, and higher IC50 value of oxaliplatin. The six-feature logistic scoring model has certain clinical significance in colon cancer.