Pan-cancer analysis of natural killer (NK) cell infiltration in human malignancies: Molecular features and clinical implications.
Authors: Zorko Nick , Hwang Justin , Lozada John R , Seifert Hilary , Elliott Andrew , Radovich Milan , Sledge George W. , Felices Martin , Heath Elisabeth I. , Hoon Dave S. B. , El-Deiry Wafik S. , Miller Jeffrey , Antonarakis Emmanuel S. 
Published: June 2023

2563 Background: NK cells are part of the innate immune system that are not antigen-specific, but can be redirected to targets of interest using multiple strategies. Advantages of NK cells over T cells include the use of allogeneic off-the-shelf products and lower risk of cytokine release syndrome. Numerous NK-specific immunotherapies are under development for the treatment of cancer, although none are yet FDA-approved. Here, we conducted a pan-cancer analysis of NK cell abundance in >90,000 tumor samples across 45 cancer types using the Caris Precision Oncology Alliance (POA) database. Features of prostate cancer (PCa, n=3365) and renal cell carcinoma (RCC, n=1106) were explored in depth. Methods: DNA (targeted/whole-exome) and RNA (whole-transcriptome) sequencing was performed for patient tumors (N=90,916) submitted to Caris Life Sciences (Phoenix, AZ). NK cell fractions were inferred from RNA-seq data using quanTIseq (Finotello, 2019). Real-world overall survival (OS) was determined from insurance claims, and Kaplan-Meier estimates were calculated. Statistical significance was determined using X2 and Mann-Whitney U tests with corrections for multiple hypothesis testing where appropriate. Results: Median NK cell fractions ranged from 7-9% (medulloblastoma and gliomas) to 2% (thyroid and thymic cancers), with intermediate levels observed for PCa (4.6%) and RCC (3.1%). High (> median) NK cell fractions were associated with improved OS (hazard ratios, 0.28–0.84, p<0.05) for 28 of 45 cancer types, while 16 of 45 including low-grade glioma, GIST, and thyroid had HR <1.0 (0.264-0.997) with 95% CI crossing 1.0. Improved OS was notable in PCa (HR 0.46, 95% CI 0.38–0.56, p<0.0001) and RCC (HR 0.52; 95% CI 0.39–0.70, p<0.0001). Unexpectedly, tumors with higher NK infiltrates were less frequently PD-L1+ (SP142) by IHC in PCa (2.8% vs 5.4%, p=0.08) and RCC (12.4% vs 30.8%, p<0.0001) ( i.e. an inverse relationship), despite a lack of correlation of NK infiltration with dMMR/MSI status or tumor mutation burden (TMB) in both cancers. Conversely, increased NK infiltration was associated with a 1.4- to 2.1–fold increased mRNA expression of immunomodulatory receptors LAG3 and TIGIT in both PCa (p<0.0001) and RCC (p<0.0001), but a 1.4–fold decrease in B7-H3 in RCC (p<0.0001). Conclusions: High NK cell infiltration is associated with improved OS in numerous cancer types, including many with inadequate therapeutic options. Our findings suggest broad deployment of NK engagers and CAR-NK products in a range of malignancies. The positive correlation between NK cells and LAG3/TIGIT suggest that combination approaches may be warranted. The inverse relationship between NK infiltrates and PD-L1, and lack of association with standard immune therapy markers (MSI status, TMB), indicates the potential use of NK cell approaches in situations where other immunotherapies are ineffective.