bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-disaggregated outcomes of adverse events after COVID-19 vaccination: A Dutch cohort study and review of the literature
Authors: Duijster Janneke W. , Lieber Thomas , Pacelli Silvia , Van Balveren Leontine , Ruijs Loes S. , Raethke Monika , Kant Agnes , Van Hunsel Florence 
Published: January 2023

BackgroundAlbeit the need for sex-disaggregated results of adverse events after immunization (AEFIs) is gaining attention since the COVID-19 pandemic, studies with emphasis on sexual dimorphism in response to COVID-19 vaccination are relatively scarce. This prospective cohort study aimed to assess differences in the incidence and course of reported AEFIs after COVID-19 vaccination between males and females in the Netherlands and provides a summary of sex-disaggregated outcomes in published literature.MethodsPatient reported outcomes of AEFIs over a six month period following the first vaccination with BioNTech-Pfizer, AstraZeneca, Moderna or the Johnson&amp;Johnson vaccine were collected in a Cohort Event Monitoring study. Logistic regression was used to assess differences in incidence of ‘any AEFI’, local reactions and the top ten most reported AEFIs between the sexes. Effects of age, vaccine brand, comorbidities, prior COVID-19 infection and the use of antipyretic drugs were analyzed as well. Also, time-to-onset, time-to-recovery and perceived burden of AEFIs was compared between the sexes. Third, a literature review was done to retrieve sex-disaggregated outcomes of COVID-19 vaccination.ResultsThe cohort included 27,540 vaccinees (38.5% males). Females showed around two-fold higher odds of having any AEFI as compared to males with most pronounced differences after the first dose and for nausea and injection site inflammation. Age was inversely associated with AEFI incidence, whereas a prior COVID-19 infection, the use of antipyretic drugs and several comorbidities were positively associated. The perceived burden of AEFIs and time-to-recovery were slightly higher in females.DiscussionThe results of this large cohort study correspond to existing evidence and contribute to the knowledge gain necessary to disentangle the magnitude of the effect sex in response to vaccination. Whilst females have a significant higher probability of experiencing an AEFI than males, we observed that the course and burden is only to a minor extent different between the sexes.
Correlation of microbiomes in “plant-insect-soil” ecosystem
Authors: Li Guomeng , Liu Peng , Zhao Jihan , Su Liangyinan , Zhao Mengyu , Jiang Zhengjie , Zhao Yang , Yang Xiping 
Published: January 2023

IntroductionTraditional chemical control methods pose a damaging effect on farmland ecology, and their long-term use has led to the development of pest resistance.MethodsHere, we analyzed the correlations and differences in the microbiome present in the plant and soil of sugarcane cultivars exhibiting different insect resistance to investigate the role played by microbiome in crop insect resistance. We evaluated the microbiome of stems, topsoil, rhizosphere soil, and striped borers obtained from infested stems, as well as soil chemical parameters.Results and DiscussionResults showed that microbiome diversity was higher in stems of insect-resistant plants, and contrast, lower in the soil of resistant plants, with fungi being more pronounced than bacteria. The microbiome in plant stems was almost entirely derived from the soil. The microbiome of insect-susceptible plants and surrounding soil tended to change towards that of insect-resistant plants after insect damage. Insects’ microbiome was mainly derived from plant stems and partly from the soil. Available potassium showed an extremely significant correlation with soil microbiome. This study validated the role played by the microbiome ecology of plant–soil-insect system in insect resistance and provided a pre-theoretical basis for crop resistance control.
Dose evaluations of organs at risk and predictions of gastrointestinal toxicity after re-irradiation with stereotactic body radiation therapy for pancreatic cancer by deformable image registration
Authors: Cao Yangsen , Zhu Xiaofei , Yu Chunshan , Jiang Lingong , Sun Yongjian , Guo Xueling , Zhang Huojun 
Published: January 2023

PurposeRe-irradiation of locally recurrent pancreatic cancer may be an optimal choice as a local ablative therapy. However, dose constraints of organs at risk (OARs) predictive of severe toxicity remain unknown. Therefore, we aim to calculate and identify accumulated dose distributions of OARs correlating with severe adverse effects and determine possible dose constraints regarding re-irradiation.MethodsPatients receiving two courses of stereotactic body radiation therapy (SBRT) for the same irradiated regions (the primary tumors) due to local recurrence were included. All doses of the first and second plans were recalculated to an equivalent dose of 2 Gy per fraction (EQD2). Deformable image registration with the workflow “Dose Accumulation-Deformable” of the MIM® System (version: 6.6.8) was performed for dose summations. Dose–volume parameters predictive of grade 2 or more toxicities were identified, and the receiver operating characteristic (ROC) curve was used to determine optimal thresholds of dose constraints.ResultsForty patients were included in the analysis. Only the V10 of the stomach [hazard ratio (HR): 1.02 (95% CI:1.00–1.04), P = 0.035] and Dmean of the intestine [HR: 1.78 (95% CI: 1.00–3.18), P = 0.049] correlated with grade 2 or more gastrointestinal toxicity. Hence, the equation of probability of such toxicity was P=11+e−(−4.155+0.579Dmean of the intestine+0.021V10 of the stomach) Additionally, the area under the ROC curve and threshold of dose constraints of V10 of the stomach and Dmean of the intestine were 0.779 and 77.575 cc, 0.769 and 4.22 Gy3 (α/β = 3), respectively. The area under the ROC curve of the equation was 0.821.ConclusionThe V10 of the stomach and Dmean of the intestine may be vital parameters to predict grade 2 or more gastrointestinal toxicity, of which the threshold of dose constraints may be beneficial for the practice of re-irradiation of locally relapsed pancreatic cancer.
Engineered octave frequency comb in integrated chalcogenide dual-ring microresonators
Authors: Wang Zifu , Luo Liyang , Xia Di , Lu Siqi , Lin Guosheng , Gao Shecheng , Li Zhaohui , Zhang Bin 
Published: January 2023

Octave-spanning Kerr combs bridging the spectral windows of the near-infrared region (NIR) and the mid-infrared (MIR) region are expected in a number of applications, including high-capacity coherent optical communications, and gas molecular absorption footprints. Here, we propose novel concentric dual-ring microresonators (DRMs) for advanced dispersion engineering to tailor the comb spectral profile. The dispersion can be flexibly engineered not only by the cross-section of the DRMs, but also by the gap between concentric dual-ring microresonators, which provides a new path to geometrically control the spectral profile of the soliton Kerr combs. An octave-spanning Kerr soliton microcomb with multi-dispersive waves has been achieved numerically covering from the telecommunication band (1224 nm) to the mid-infrared band region (2913 nm) with a −40 dB bandwidth of 1265 nm. Our results are promising to fully understand the nonlinear dynamics in hybrid modes in DRMs, which helps control broadband comb formation.
Transcriptomics analysis of ethanol treatment of male Aedes aegypti reveals a small set of putative radioprotective genes
Authors: Pinch Matthew , Bendzus-Mendoza Harley , Hansen Immo A. 
Published: January 2023

Introduction: Sterile Insect Technique (SIT) is based on releasing sterilized male insects into wild insect populations to compete for mating with wild females. Wild females mated with sterile males will produce inviable eggs, leading to a decline in population of that insect species. Sterilization with ionizing radiation (x-rays) is a commonly used mechanism for sterilization of males. Since irradiation can cause damage to both, somatic and germ cells, and can severely reduce the competitiveness of sterilized males relative to wild males, means to minimize the detrimental effects of radiation are required to produce sterile, competitive males for release. In an earlier study, we identified ethanol as a functional radioprotector in mosquitoes.Methods: Here, we used Illumina RNA-seq to profile changes in gene expression of male Aedes aegypti mosquitoes fed on 5% ethanol for 48 hours prior to receiving a sterilizing x-ray dose, compared to males fed on water prior to sterilization.Results: RNA-seq revealed a robust activation of DNA repair genes in both ethanol-fed and water-fed males after irradiation, but surprisingly few differences in gene expression between ethanol-fed and water-fed males regardless of radiation treatment.Discussion: While differences in gene expression due to ethanol exposure were minimal, we identified a small group of genes that may prime ethanol-fed mosquitoes for improved survivability in response to sterilizing radiation.
Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses
Authors: Shang Heyang , Fang Linqi , Qin Lifang , Jiang Hongtao , Duan Zhenzhen , Zhang Hai , Yang Zongtao , Cheng Guangyuan , Bao Yixue , Xu Jingsheng , Yao Wei , Zhang Muqing 
Published: January 2023

IntroductionPlant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses.MethodsThe class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative PCR.ResultsEighty-two PRX proteins were characterized with a conserved PRX domain as members of the class III PRX gene family in R570 STP. The ShPRX family genes were divided into six groups by the phylogenetic analysis of sugarcane, Saccharum spontaneum, sorghum, rice, and Arabidopsis thaliana. The analysis of promoter cis-acting elements revealed that most ShPRX family genes contained cis-acting regulatory elements involved in ABA, MeJA, light responsiveness, anaerobic induction, and drought inducibility. An evolutionary analysis indicated that ShPRXs was formed after Poaceae and Bromeliaceae diverged, and tandem duplication events played a critical role in the expansion of ShPRX genes of sugarcane. Purifying selection maintained the function of ShPRX proteins. SsPRX genes were differentially expressed in stems and leaves at different growth stages in S. spontaneum. However, ShPRX genes were differentially expressed in the SCMV-inoculated sugarcane plants. A qRT-PCR analysis showed that SCMV, Cd, and salt could specifically induce the expression of PRX genes of sugarcane.DiscussionThese results help elucidate the structure, evolution, and functions of the class III PRX gene family in sugarcane and provide ideas for the phytoremediation of Cd-contaminated soil and breeding new sugarcane varieties resistant to sugarcane mosaic disease, salt, and Cd stresses.
Metabolites and Plant Hormones Related to the Resistance Response to Feeding Stimulation and Leaf Clipping Control in Chinese Pine (Pinus tabuliformis Carr.)
Authors: Zhao Yanan , Zhou Guona , Sun Tianhua , Wang Lifeng , Xu Qiang , Liu Junxia , Gao Baojia 
Published: January 2023

This experiment was conducted to define changes in metabolic pathways in response to mandibulate insect feeding and to provide a reference for further investigation of the molecular mechanisms underlying the development of conifer resistance. Chinese pine (Pinus tabuliformis Carr.) in good growth status in natural condition was chosen for stimulation by 10 pine caterpillars (Dendrolimus tabulaefomis Tsai et Liu) as feeding stimulation (FS), leaf clipping control (LCC) as mechanical damage, and CK group (with no treatment) (recorded as 0 h). The metabolome and total flavonoid content were measured in the needles at 0, 2, and 8 h after treatment. Plant hormones were measured with needles at 0, 0.5, 1, 1.5, 2, 4, 6, and 8 h after different treatments. The results show that a total of 30.8% flavonoids are identified by metabolomics analysis. Compared with leaf clipping control, feeding stimulation of Chinese pine caterpillars significantly induced the upregulation of metabolites in the flavonoid pathway in Chinese pine, and the plant hormones JA and IAA showed expression trends consistent with those of the metabolome. According to the biological processes of the four plant hormones involved, JA and SA are mostly involved in resistance formation, and in this study, both of them also have fluctuating expressions influenced by feeding stimulation, while the expressions of the growth-related hormones IAA and ABA have no significant changes at other time points except for 1 h after treatment. Thus, the flavonoid pathway is one of the main pathways involved in resistance formation in conifers, and JA and IAA are involved in the formation of resistance.
Effect of Thermal Exposure on Mechanical Properties of Al-Si-Cu-Ni-Mg Aluminum Alloy
Authors: Chen Fanming , Liu Chengwen , Zuo Lijie , Wu Zhiyuan , He Yiqiang , Dong Kai , Li Guoqing , He Weiye 
Published: January 2023

The microstructure morphology and evolution of mechanical properties are investigated in this study. The results show that the phases displayed no clear change after thermal exposure at 250 °C for 200 h. The tensile strength of the as-cast alloy showed a downward trend in different degrees with the increase in the tensile temperature, while the influence of elongation was opposite to the tensile strength. In addition, the tensile strength tended to be stable after thermal exposure at 250 °C for 100 h. The main creep mechanism of the as-cast alloy at a low temperature and low stress (T ≤ 250 °C; σ ≤ 40 MPa) is grain-boundary creep. The Monkman–Grant empirical formula was used to fit the relationship between the creep life and the minimum creep rate, and the fitting results are: tr·ε˙min0.95=0.207.
Response Surface Methodology for Optimization of Multiplex-PCR Protocols for Detection of TYLCV, TSWV and Fol Molecular Markers: Analytical Performance Evaluation
Authors: Lafrance Richecarde , Valdez-Torres José Benigno , Villicaña Claudia , García-Estrada Raymundo Saúl , Esparza-Araiza Mayra Janeth , León-Félix Josefina 
Published: January 2023

Molecular markers linked to disease resistance genes which affect economically important crops are of great interest. In the case of tomato, a major focus on resistance breeding to multiple fungal and viral pathogens such as Tomato yellow leaf curl virus (TYLCV), Tomato spotted wilt virus (TSWV) and Fusarium oxysporum f. sp. lycopersici (Fol), have led to the introgression of several resistance genes; therefore, molecular markers have become important in molecular-assisted selection (MAS) of tomato varieties resistant to those pathogens. However, assays that allow simultaneous evaluation of resistant genotypes, such as multiplex PCR, need to be optimized and evaluated to demonstrate their analytical performance, as many factors can affect them. This work aimed to generate multiplex PCR protocols for the joint detection of the molecular markers associated with pathogen resistance genes in tomato plants that are sensitive, specific and repeatable. For the optimization a central composite design of a response surface methodology (RSM-CCD) was used. For analytical performance evaluation, specificity/selectivity and sensibility (limit of detection and dynamic range) were analyzed. Two protocols were optimized: the first one with a desirability of 1.00, contained two markers (At-2 and P7-43) linked to I- and I-3-resistant genes. The second one with a desirability of 0.99, contained markers (SSR-67, SW5 and P6-25) linked to I-, Sw-5-, and Ty-3-resistant genes. For protocol 1, all the commercial hybrids (7/7) were resistant to Fol, and for protocol 2, two hybrids were resistant to Fol, one to TSWV and one to TYLCV with good analytical performance. In both protocols, the varieties considered susceptible to the pathogens, no-amplicon or susceptible amplicons, were observed. The optimized multiplex PCR protocols showed dynamic ranges from 5.97 up to 161.3 ng DNA. The limit of detection was 17.92 ng and 53.76 ng DNA for protocols 1 and 2, respectively, giving 100% positive results in the test replicates. This method allowed to develop optimized multiplex PCR protocols with few assays which translates into less time and resources, without sacrificing method performance.
Proteome Analysis of Male Accessory Gland Secretions in the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)
Authors: Wu Li-Juan , Li Fan , Song Yue , Zhang Zhan-Feng , Fan Yong-Liang , Liu Tong-Xian 
Published: January 2023

In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs are transferred along with sperms inside female bodies and have a significant impact on the post-mating physiology changes of the females. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and vary from species to species. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. Mating has a profound impact on the females’ behavior and physiology in this species. It is still unclear what the ACPs are in this species. In this study, two different proteomic methods were used to identify ACPs in P. xylostella. The proteins of MAGs were compared immediately before and after mating by using a tandem mass tags (TMT) quantitative proteomic analysis. The proteomes of copulatory bursas (CB) in mated females shortly after mating were also analyzed by the shotgun LC-MS/MS technique. In total, we identified 123 putative secreted ACPs. Comparing P. xylostella with other four insect ACPs, trypsins were the only ACPs detected in all insect species. We also identified some new insect ACPs, including proteins with chitin binding Peritrophin-A domain, PMP-22/ EMP/ MP20/ Claudin tight junction domain-containing protein, netrin-1, type II inositol 1,4,5-trisphosphate 5-phosphatase, two spaetzles, allatostatin-CC, and cuticular protein. This is the first time that ACPs have been identified and analyzed in P. xylostella. Our results have provided an important list of putative secreted ACPs, and have set the stage for further exploration of the functions of these putative proteins in P. xylostella reproduction.
Functional Expression of Recombinant Candida auris Proteins in Saccharomyces cerevisiae Enables Azole Susceptibility Evaluation and Drug Discovery
Authors: Toepfer Stephanie , Lackner Michaela , Keniya Mikhail V. , Monk Brian C. 
Published: January 2023

Candida auris infections are difficult to treat due to acquired drug resistance against one or multiple antifungal drug classes. The most prominent resistance mechanisms in C. auris are overexpression and point mutations in Erg11, and the overexpression of efflux pump genes CDR1 and MDR1. We report the establishment of a novel platform for molecular analysis and drug screening based on acquired azole-resistance mechanisms found in C. auris. Constitutive functional overexpression of wild-type C. auris Erg11, Erg11 with amino acid substitutions Y132F or K143R and the recombinant efflux pumps Cdr1 and Mdr1 has been achieved in Saccharomyces cerevisiae. Phenotypes were evaluated for standard azoles and the tetrazole VT-1161. Overexpression of CauErg11 Y132F, CauErg11 K143R, and CauMdr1 conferred resistance exclusively to the short-tailed azoles Fluconazole and Voriconazole. Strains overexpressing the Cdr1 protein were pan-azole resistant. While CauErg11 Y132F increased VT-1161 resistance, K143R had no impact. Type II binding spectra showed tight azole binding to the affinity-purified recombinant CauErg11 protein. The Nile Red assay confirmed the efflux functions of CauMdr1 and CauCdr1, which were specifically inhibited by MCC1189 and Beauvericin, respectively. CauCdr1 exhibited ATPase activity that was inhibited by Oligomycin. The S. cerevisiae overexpression platform enables evaluation of the interaction of existing and novel azole drugs with their primary target CauErg11 and their susceptibility to drug efflux.
Risk Factors and the Character of Clinical Course of the Echinococcus multilocularis Infection in Patients in Poland
Authors: Stefaniak Magdalena , Derda Monika , Zmora Pawel , Nowak Szymon Pawel 
Published: January 2023

Alveolar echinococcosis (AE) is a chronic zoonotic disease caused by the larval form of Echinococcus multilocularis. In humans, it may become a serious chronic infection of the liver which resembles a slow malignant process leading to death when untreated. The aim of the study was an assessment of the risk factors of the E. multilocularis infections and the description of AE clinical course in the group of 36 patients with confirmed AE, hospitalized at the Department and Clinic of Tropical and Parasitic Diseases, Poznan University of Medical Sciences between 2013 and 2022. Among the study participants, most patients cultivated land, bred livestock, worked in the forest, or were employed in animal shelters. The E. multilocularis infection was diagnosed based on imaging and immunoassay techniques within 6 months in the majority of patients hospitalized in the Department. All patients hospitalized in the Department initiated anti-parasitic therapy at the moment of the diagnosis. Pharmacological treatment combined with surgery was applied in most of the study participants, who were presented with more advanced stages of infection. We conclude the following: 1. For humans in the risk group, regular abdominal imaging examinations and the detection of specific antibodies against E. multilocularis are recommended. 2. Regular screening tests in the hyperendemic areas of AE would increase the early detection of the disease and to improve the clinical prognosis in this extremely life-threatening parasitic disease.
O ambiente escolar como facilitador para o desenvolvimento moral de crianças e adolescentes
Authors: Fernandes Elen Daiane Quartaroli , Ferreira Patrícia Elisabete , Martins Raul Aragão 
Published: January 2023

Marcada pela diversidade de estilos culturas e valores, a escola se caracteriza como um espaço de conflitos e relações interpessoais. Saber como lidar com eles, de forma a utilizá-los como ferramenta de reflexão para uma consciência autônoma, se torna um desafio, mas pode favorecer a promoção da convivência dos educandos neste espaço. Essa pesquisa tem por objetivo refletir sobre a importância do ambiente escolar no desenvolvimento moral de crianças e adolescentes, a partir de uma discussão teórica sobre o tema e da análise de duas experiências, bem-sucedidas, sobre como a escola pode influenciar positivamente na construção da autonomia de seus alunos, sendo uma com crianças e a outra com adolescentes, com o intuito de promover novas ações que atuem diretamente nesse processo. Discutir sobre essa temática em programas de formação docente, se torna fundamental para que os profissionais da educação possam compreender como se estabelecem as relações interpessoais dentro da escola e como o manejo dos conflitos pode influenciar na construção da autonomia dos estudantes, facilitando assim, a convivência entre eles e o desenvolvimento da criticidade. Com base na teoria piagetiana, constatou-se que o desenvolvimento moral é resultado de um processo contínuo que ocorre por meio das relações estabelecidas entre os indivíduos e que a escola, por sua vez, é um espaço privilegiado e coletivo em que as relações são exercitadas em direção a uma construção de valores socialmente desejáveis, com respeito às diversidades e igualdade, possibilitando uma convivência mais humana e democrática entre os alunos.