bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biodegradation of Tetracycline and Ciprofloxacin in aerobic composting using different microbial sources
Authors: Mousavi Seyedeh Fatemeh , Zazouli Mohammad Ali , Gholami-Borujeni Fathollah 
Published: August 2023

Abstract
The objective of this study was to evaluate the degradation of tetracycline (TC) and ciprofloxacin (CIP) during in-vessel composting in three different ratios of organic waste (OW), rice husk (RH), and return activated sludge (RAS) obtained from antibiotic industry wastewater treatment plant as a microbial source. The composting process was carried out in three cylindrical reactors (R1, R2, and R3) with a volume of 35.5 L. The OW/RH ratio was adjusted to 2:1, 1.4:1, and 1:1, and the RAS was added as a microbial source and to adjust the moisture content (MC). After the compost maturation phase, various concentrations of antibiotics were added to the different reactors. The HPLC method was used to measure the concentration of residual antibiotics. The physical, chemical, and bacterial properties of the compost were also analyzed throughout the process using standard methods. The findings showed that the removal efficiencies of TC were 85%, 90%, and 92.5% in R1, R2, and R3, respectively, while those of CIP were 75%, 77.5%, and 82.5% after 92 days. It was observed that using microbial sources containing antibiotic-resistant bacteria (sludge from an antibiotic wastewater treatment plant), fungi, and actinomycetes in the composting process can be an effective method for the biodegradation of antibiotics in contaminated environments.
RHC genotyping in Chinese Han population
Authors: Shao Lin-Nan , Zheng Zi-Wei , Zhou Shi-Hang , Zhang Shu-Ting , Song Wen-Qian , Xia Yue-Xin , Liang Xiao-Hua 
Published: August 2023

Abstract
The Rh blood group is a complex, polymorphic system with 56 antigens. Accurately predicting the presence of the C antigen using genotyping methods has been challenging. This study aimed to evaluate different genotyping methods for C predicting and identifying associated genetic variations in the Chinese Han population. In total, 317 donors, including 223 D+ (including 20 with the Del phenotype) and 94 D− were randomly selected. Real-time PCR of the 48C and 109bp insertion, and restriction fragment length polymorphism of −292 substitutions were used for RHC genotyping. Moreover, the promoter region of the RHCE gene was sequenced to search for other nucleotide substitutions between RHC and RHc. The 109bp insertion method showed the highest accuracy in predicting the C phenotype. Sequencing showed that seven SNPs in the promoter and exon 1 of the RHCE gene were associated with the C phenotype. However, these SNPs also showed a few false positive results. The accuracy of the SNPs in predicting C, in descending order, was found to be rs586178>rs4649082, rs2375313, rs2281179, rs2072933, and rs2072932>rs2072931. Although the identified SNPs alone cannot independently and accurately correlate with the C phenotype, combining them with the 109bp insertion test can improve the accuracy of predicting C.
Continuous-discrete extended Kalman filter based parameter identification method for space robots in postcapture
Authors: Zhang Teng , Shi Peng , Yang Yang , Li Wenlong , Yue Xiaokui 
Published: August 2023

Abstract

Space robots have become increasingly important in on-orbit missions, especially for capturing noncooperative targets. However, a major challenge is that the inertial parameters of these targets are often unknown, but crucial for post-capture tasks. This paper proposes continuous-discrete extended Kalman filter (CD-EKF) based identification methods that rely solely on noisy measurements of the manipulator’s rotation angle, the base’s attitude angle, and its position. The methods exploit the conservation of momentum or the dynamics of space robots to formulate the identification equations and construct the filter. In addition, an analytical solution for computing the Jacobian matrix of the dynamic response of a space robot is derived, and sparse matrix multiplication is used to reduce the computation time. The effectiveness and efficiency of the proposed methods are evaluated through numerical simulations using 2D and 3D models, and Monte Carlo simulations are performed to analyze robustness, noise effects, and initial states. The simulation results confirm the effectiveness of the proposed methods and demonstrate their ability to handle noise and uncertainty.
Effectiveness of a structured pharmaceutical review of pharmacotherapeutic plans to reduce drug-related problems in care homes: a pragmatic quasi-experimental trial
Authors: Prats Cecília Campabadal , Aragonès Enric , Romeu Marta , Salom-Garrigues Clara , Bejarano Ferran , Canadell Laura 
Published: August 2023

Abstract
Background: Patients living in Geriatric Residences (RG) have increased aging, comorbidity, spending on health resources and mortality. High drug use is associated with an increased risk of falls, disability, and death. It is estimated that 20-50% of inappropriate medications are consumed in the elderly.
Methodology:
Hypothesis: A pharmaceutical intervention based on a clinical review of the pharmacotherapeutic plan of patients in RG will be useful in optimizing the prescription in terms of safety, reducing by 10% or more the inappropriate prescriptions.
Objectives:Evaluate the effectiveness of a structured pharmaceutical intervention based on the clinical review of the pharmacotherapeutic plan to improve its adequacy.
Decrease inadequate prescriptions in patients admitted to RG by 10%
Pre-post, quasi-experimental intervention study with control group with prospective follow-up of a cohort of patients in RG. The intervention consists of the clinical review of the pharmacotherapeutic plan carried out by the pharmacist and subsequently agreed in the Pharmacotherapeutic Advisory Committee.
Determinations: The study variables will be evaluated at baseline and 3 months post-intervention. The primary variable is number of inappropriate prescriptions.
Statistical analysis: Percentage change will be measured before and after the intervention. Descriptive statistics will be performed for quantitative variables such as qualitative and comparison of means and proportions.
Expected results: Achieve a 10% decrease in inappropriate prescriptions
Applicability and Relevance: This study will expand the collaboration between the Pharmacy and Primary Care physicians, promoting the continuum of care, strengthening the safety culture, and improving prescribing habits.
Ethics: The protocol was approved by the Research Ethics Committee of the Jordi Gol Primary Care Research Institute (IDIAP), Barcelona, 22/191-EOm.
Trial registration: ClinicalTrials.gov: NCT05944328, Registered
Microwave ablation of benign thyroid nodules: the importance of modified hydrodissection
Authors: kucukay fahrettin , acu berat , subasi berkay 
Published: August 2023

Abstract
Purpose: To evaluate the safety and efficacy of microwave ablation (MWA) in treating benign thyroid nodules and any advantage of modified hydrodissection (MHD) techniques while performing these procedures.
Materials and Methods: This retrospective study included 76 consecutive patients treated for benign thyroid nodules using MWA. Patients were divided into two groups. Group 1 included patients treated before November 2019 using the HD technique (n=38), and group 2 included patients treated using the MHD technique after November 2019 (n=38). HD-MHD was performed using 5% dextrose. A portable MWA device with a 2450 MHz frequency was used as the generator. MWA antennas with internal water-cooled units and temperature monitoring features with a 16G diameter, 100 cm body length, and 3 mm tip length were used for MWA procedures. Color Doppler ultrasound examinations, thyroid function tests, volume reduction ratios (VRR), visual analog scales, and cosmetic scores were performed on the patients in the first, third, sixth, and every sixth months after ablation procedures. Complications and procedure times were also recorded.
Results: No significant differences in VRR, visual analog scale, or cosmetic score were observed between the groups at 3, 6, and 12 months. Lower complication rates ( 26.31% vs. 7.89%, p=0.03) and procedure times (10.30 min vs. 7.30 min; p=0.04) were observed with the MHD technique. There were no residual or recurrent nodules or thyroid hormone deficiencies during the follow-up.
Conclusion: MWA of benign thyroid nodules is safe and effective. Using the MHD technique lowers complication rates and procedure time.
Pharmacological evaluation of Natural Sea Salt against doxorubicin induced cardiac toxicity via Gut Microbiome
Authors: Asar Turky Omar , Nadeem Muhammad Shahid , Abbasi Fahad A. Al , Sheikh Ryan Adnan , Naqvi Salma , Hosawi Salman Bakr I. , Almalki Naif Abdullah R. , Alhayyani Sultan , Kumar Vikas , Rizwanuddin Sheikh , Kaleem Mohammed , Anwar Firoz 
Published: August 2023

Abstract
Cardiovascular complications including cardiac toxicity pose a major health threat worldwide. These complications can be handled by reducing the associated risk factors including diet. Gut microbiome is prominently associated with cardiovascular diseases. The present study demonstrates how the gut microbiota, under the effect of table salt and natural sea salt, modulates doxorubicin-induced cardiac toxicity. Experimental animals were divided into six groups (n = 6) as: Group I. Normal control (NC); Group II: table salt (0.3%) (NTS); Group III: natural sea salt (0.3%) (NSS.); Group IV: Doxorubicin-induced cardiac toxicity control (2mg/kg) (DC); Group V:DC + Table salt (0.3%) (DTS.); and Group VI: DC + Natural sea salt (0.3%) (DSS). CKMB, CRP, lipid profile was assayed in serum, GUT microbiota in stool, and histopathological variations in heart tissues were studied. Significant alterations were observed in the analytical values of blood parameters in the doxorubicin versus the normal control group. Also, a significant variation was observed in DTS and DSS groups for CKMB (p < 0.001), CRP (p < 0.01; p < 0.05), TC, TG, LDL (p < 0.0001) and HDL (p < 0.05, p < 0.01) respectively, when compared with DC group. Presence of gut microbes were identified in the stool samples. The population of Proteobacteria and Spirochetes were significantly lowered the DC group as compared to all other groups. However, the treatment with the sea salt increased the richness of this phylum to 36-fold, while table salt increased only by 9-fold. The change in microbial population is a direct marker for cardiac toxicity, which was highly prevented by sea salt as compared to table salt. Histopathological alterations in cellular architecture of the heart reflect a marked effect of the salts on it. Our observations suggest that a diet with natural sea salt demonstrated a significant protection to cardiac toxicity that may have initiated via inflammation after doxorubicin injury compared to the table salt.
Enhanced Photocatalytic activity of bio mediated Ag@Cu2O NPs towards Fampridine: Kinetic modelling and mechanistic pathways
Authors: ATTRI PRATIBHA , Garg Sangeeta , Rata Jatinder Kumar , Giri Ardhendu Sekhar 
Published: August 2023

Abstract

To enhance the photocatalytic activity of Cu2O NPs, Tabernaemontana divaricate leaf extract was used to synthesise Ag@Cu2O NPs. UV-Vis, XPS, XRD, and HR-TEM were used to characterize Ag@Cu2O NPs. The mechanism of Ag+ binding onto Cu2O NPs showed that plant extract was fully saturated with flavonoids and can implant Ag on the Cu2O NPs. XPS spectra of Ag@Cu2O NPs showed a shift towards lower binding energy in Cu 2p peaks from 932 eV to 931 eV and 952 eV to 951 eV, which confirmed the formation of Ag@Cu2O NPs. HR-TEM investigation of Ag@Cu2O NPs indicated that the lattice distance was (d = 0.25 nm) which corresponds to the (111) plane of Ag, which demonstrated the fabrication of Ag@Cu2O NPs. The degradation efficiency of fampridine was enhanced to 95% with 97% COD removal and 75% TOC conversion after 210 min using Ag@Cu2O NPs as compared to Cu2O NPs. The MFOK model was well fitted for the TOC conversion of fampridine, with R2 = 0.99 in comparison to the FOK model. LC-MS spectra for the degradation of fampridine have shown ten intermediate fragments. Ag@Cu2O NPs that were synthesised effectively inhibited the bacterial pathogen.
Cost-utility Analysis of Adult Patients with Severe Aplastic Anemia: a Single-center Cohort Study
Authors: Chen Lin , Fang Liwei , Kuang Zhexiang , Xu Jing , Wang Chuan 
Published: August 2023

Abstract
Background: This study aimed to evaluate the efficacy of cyclosporine A + ALG and cyclosporine A + TPO-RA in treating SAA (Severe Aplastic Anemia) patients and provide scientific data support for maximizing the therapeutic benefit of SAA patients.
Methods: Cost-utility analysis was used to compare the two strategies' quality-adjusted years of life and total costs. Patient utility values were obtained by EQ-5D-3L and Japanese TTO conversion, and total treatment costs were obtained by electronic questionnaire and medical information data.
Results: Twenty-six adult patients were enrolled, including 17 patients in the cyclosporine A + ALG group and nine in the cyclosporine A + TPO-RA group. From admission to 6 months follow-up, the intuitive feelings of patients in the cyclosporine A + ALG group were higher than of those in the cyclosporine A + TPO-RA group, and the quality of life of the former group was significantly higher than that of the latter, with a difference of 0. 08 QALY (P < 0.01). The total cost of the former was twice that of the latter. The QALYs obtained by the two strategies were 687,000 yuan and 450,000 yuan, respectively. Compared with the cyclosporine A + TPO-RA regimen, the total cost of the cyclosporine A + ALG regimen was 1.51 million yuan per additional QALY.
Conclusions: Under the premise of ensuring clinical efficacy, CsA + TPO-RA can be used as a better treatment strategy to reduce the economic pressure of SAA patients, reduce the pressure of medical insurance, and obtain multi-benefits.
Ultrasound evaluation of cardiac and diaphragmatic function at different positions during a spontaneous breathing trial predicting extubation outcomes: a retrospective cohort study
Authors: Luo Ling , Li Yi-dan , Wang Li-fang , Sun Bing , Tong Zhao-hui 
Published: August 2023

Abstract

Background: The ratio (E/Ea) of mitral Doppler inflow velocity to annular tissue Doppler wave velocity by transthoracic echocardiography and diaphragmatic excursion (DE) by diaphragm ultrasound have been confirmed to predict extubation outcomes. However, few studies focused on the predicting value of E/Ea and DE at different positions, and the effects of △E/Ea and △DE [the changes of E/Ea and DE during a spontaneous breathing trial (SBT)].
Methods: This study was a reanalysis of the data of 60 difficult-to-wean patients in a previous study published in 2017. All eligible participants were divided into respiratory failure (RF) group and extubation success (ES) group within 48 hours, or re-intubation (RI) group and non-intubation (NI) group within 1 week. The risk factors for respiratory failure and re-intubation including E/Ea and △E/Ea, DE and△DE at different positions were analyzed by multivariate logistic regression, respectively. The receiver operating characteristic (ROC) curves of E/Ea (septal, lateral, average) and DE (right, left, average) were compared with each other. Combination of E/Ea and △E/Ea, or DE and △DE was analyzed to predict respiratory failure or re-intubation.
Results: Among 60 patients, 29 cases developed respiratory failure, and 14 cases were re-intubated. Multivariate logistic regression showed that E/Ea were all associated with respiratory failure, while only DE (right) and DE (average) after SBT were related to re-intubation. There were no statistic differences between the ROC curves of E/Ea and DE at different positions. No statistical differences were shown in △E/Ea between RF and ES groups, but △DE (average) was remarkably higher in NI group than that in RI group. There was a tendency that the combined factor could predicted respiratory failure better than E/Ea (average) after SBT or △E/Ea (average) , same as for the combined factor predicting re-intubation better than DE (average) after SBT or △DE (average).
Conclusions: Cardiac and diaphragmatic dysfunction were related topostextubation respiratory failure and re-intubation, respectively. There were no statistical differences in E/Ea at different positions, similarly with DE. The combined factors could show the highest diagnostic accuracy in predicting respiratory failure and re-intubation.
Pre-movement sensorimotor oscillations shape the sense of agency by gating cortical connectivity
Authors: Bertoni Tommaso , Noel Jean-Paul , Bockbrader Marcia , Foglia Carolina , Colachis Sam , Orset Bastien , Rezai Ali , Panzeri Stefano , Becchio Cristina , Blanke Olaf , Serino Andrea 
Published: August 2023

Abstract
Our sense of agency, the subjective experience of controlling our actions, is a crucial component of self-awareness and motor control. It is thought to originate from the comparison between intentions and actions across broad cortical network. However, the underlying neural mechanisms are still unknown. We hypothesized that oscillations in the theta-alpha range, thought to orchestrate long-range neural connectivity, may mediate sensorimotor comparisons. To test this, we manipulated the relation between intentions and actions in a tetraplegic user of a brain machine interface (BMI), decoding primary motor cortex (M1) activity to restore hand functionality. We found that the pre-movement phase of low-alpha oscillations in M1 predicted the participant’s agency judgements. Using EEG-BMI in healthy participants, we linked M1 and supplementary motor area (SMA) pre-movement alpha oscillations to agency ratings, and to changes in their functional connectivity with sensory areas. These findings argue for phase-driven gating as a key mechanism for sensorimotor integration and agency.
Molecular Characterization of Contagious Pustular Dermatitis Virus in goats of Sri Lanka
Authors: Piratheepan Susanthaa , Puvanendran Sumathy , Perera Kalyani 
Published: August 2023

Abstract
Contagious Pustular Dermatitis (CPD), also known as Orf or Sore Mouth, is a zoonotic disease, in small ruminants. Contagious Pustular Dermatitis Virus (CPDV) is an epitheliotrophic DNA virus belongs to the Genus Parapoxvirus DNA virus Poxviridae family. CPD is characterized by papules and pustules typically found on and around muzzle, mouth and nose and eventually develop into thick scabs which become a site for secondary bacterial infections. CPD had been reported from all parts of Sri Lanka. In the North Central, Northern and Eastern provinces where 60% of national goat population is found, its incidence is higher. Laboratory confirmation and phylogenetic analysis of CPDV obtained through PCR and DNA sequencing respectively. Ninety two scab samples from affected animals were collected in 50% Glycerol phosphate saline from the following districts: Vavuniya, Trincomalee, Anuradhapura, Jaffna, Mannar, Kilinochchi and Kandy. Viral DNA was extracted and PCR was performed with modifications by using following primers ORF2F5’-CGAACTTCCACCTCAACCACTCC-3’ and ORF2R5’-CCTTGACGACGATGTCGCCCTTCT-3’. Partial B2L gene was amplified. B2L gene, which codes for an envelope protein, is highly conserved for CPDV. Hence, ten CPD positive samples (PCR products) representing different regions such as Trincomalee, Vavuniya, Anuradhapura, Kandy Kilinochchi and Jaffna were sequenced for partial B2L gene (507bp). Laboratory analysis showed that eighty-six samples were positive for CPD viral DNA. In phylogenetic analysis based on the partial B2L gene, the Sri Lankan strain was closer to the ORFV Assam/09 isolates (JN846834) from India. A12 strain (Anuradhapura) and ORFV Assam/09 isolates showed 99% similarity at the nucleotide levels. Two isolates from India (Accession No JN 846834, KU128538) were closely related to isolates from Anuradhapura, Mannar, Jaffna and Kilinochchi districts. Two isolates from China (Accession No KU 199831 and KC 568397) were closely related to isolates from Vavuniya and Trincomalee. Sri Lankan isolates are closely related with each other. Therefore one of the strains could be used as a vaccine candidate
Real or fake? Decoding realness levels of stylized face images with EEG
Authors: Chen Yonghao , Stephani Tilman , Bagdasarian Milena Teresa , Hilsman Anna , Eisert Peter , Villringer Arno , Bosse Sebastian , Gaebler Michael , Nikulin Vadim V. 
Published: August 2023

Abstract

Artificially created human faces play an increasingly important role in our digital world. However, the so-called uncanny valley effect may cause people to perceive highly, yet not perfectly human-like faces as eerie, bringing challenges to the interaction with virtual agents. At the same time, the neurocognitive underpinnings of the uncanny valley effect remain elusive. Here, we utilized an electroencephalography (EEG) dataset of steady-state visual evoked potentials (SSVEP) in which participants were presented with human face images of different stylization levels ranging from simplistic cartoons to actual photographs. Assessing neuronal responses both in frequency and time domain, we found a non-linear relationship between SSVEP amplitudes and stylization level, that is, the most stylized cartoon images and the real photographs evoked stronger responses than images with medium stylization. Moreover, realness of even highly similar stylization levels could be decoded from the EEG data with task-related component analysis (TRCA). Importantly, we also account for confounding factors, such as the size of the stimulus face’s eyes, which previously have not been adequately addressed. Together, this study provides a basis for future research and neuronal benchmarking of real-time detection of face realness regarding three aspects: SSVEP-based neural markers, efficient classification methods, and low-level stimulus confounders.
Adrenal crisis mainly manifested as recurrent syncope secondary to Tislelizumab:a case report and literature review
Authors: Wei HaiShan , Zuo Anju , Chen Jiying , Zheng Chunyan , Li Tingting , Yu Haiyan , Guo Yuan 
Published: August 2023

Abstract
As immune checkpoint inhibitors (ICIs), Tislelizumab is an anti-programmed cell death protein 1 (PD-1) drug. With the extensive application of ICIs, there is an ever-increasing proportion of immune-related adverse events (irAEs) in clinic, some of which may even be life-threatening. Herein we present a patient of Tislelizumab-induced adrenal crisis. The main clinical manifestation is recurrent syncope accompanied by high-grade fever. Timely identification and hormone replacement therapy helped the patient get through the crisis well. Finally,the patient discontinued Tislelizumab and switch to ADC therapy.We reported this case to improve our understanding of this situation, identify this kind of disease and prevent adrenal crisis in time. Eventually, limiting toxicities to reduce the interruption of immunotherapy. Since irAEs are multisystem damage with more non-specific symptoms, except for oncologists, general practitioners who endorse the need for taking a holistic approach to the patient should play a vital role in the management of cancer treatment.