bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lifestyle Medicine Practitioners Implementing a Greater Proportion of Lifestyle Medicine Experience Less Burnout
Authors: Pollard Kathryn J. , Gittelsohn Joel , Patel Padmaja , Lianov Liana , Freeman Kelly , Staffier Kara L. , Pauly Kaitlyn R. , Karlsen Micaela C. 
Published: June 2023

Purpose To identify reasons for burnout, characterize the effect of lifestyle medicine (LM) practice on burnout, and assess the risk of burnout in relation to the proportion of LM practice. Design Analysis of mixed methods data from a large, cross-sectional survey on LM practice. Setting Web-based survey platform. Participants Members of an LM medical professional society at the time of survey administration. Methods Practitioner members of a medical professional society were recruited to a cross-sectional, online survey. Data were collected on LM practice and experiences with burnout. Free-text data were thematically grouped and counted, and the association of burnout with the proportion of lifestyle-based medical practice was analyzed using logistic regression. Results Of 482 respondents, 58% reported currently feeling burned out, 28% used to feel burned out but no longer do, and 90% reported LM had positively impacted their professional satisfaction. Among LM practitioners surveyed, practicing more LM was associated with a 43% decrease (0.569; 95% CI: 0.384, 0.845; P = 0.0051) in the odds of experiencing burnout. Top reasons for positive impact included professional satisfaction, sense of accomplishment, and meaningfulness (44%); improved patient outcomes and patient satisfaction (26%); enjoyment of teaching/coaching and engaging in relationships (22%); and helps me personally: quality of life and stress (22%). Conclusion Implementing LM as a greater proportion of medical practice was associated with lower likelihood of burnout among LM practitioners. Results suggest that increased feelings of accomplishment due to improved patient outcomes and reduced depersonalization contribute to reduced burnout.
Chemical foaming of polylactic acid/polypropylene and polylactic acid/polyamide 6: Evaluation of changes in their properties
Authors: Tuna Sibel , Akkoyun Kurtlu Meral 
Published: June 2023

Most of the polymers made from fossil fuels end up as waste material and this environment-damaging situation has revealed the need to take some precautions such as the use of eco-friendly, biodegradable materials obtained from renewable sources as an alternative. Biopolymers are being evaluated as alternatives to traditional polymers especially in the automotive industry due to their better-understood properties such as mechanical and physical behavior through the studies conducted. On the other hand, when the literature studies are evaluated, it can be seen that the production of foam materials, which are focused on lightness and carbon dioxide (CO2) emission limitations that are important and essential for the automotive sector, is an area that is also researched and studied for biopolymers. Several studies are carried out with both physical and chemical foaming agents on this subject in recent years. In this study, it is aimed to develop light, environmentally friendly, high-performance polylactic acid (PLA) based polymeric composite foams that can be used in the automotive industry by using twin screw extruder and compression molding methods. For this purpose, polyamide 6 (PA6) and polypropylene (PP) polymers were used with PLA and by adding 1 wt.%, 1.5 wt.% and 2 wt.% chemical blowing agents to the polymer mixtures, the physical, thermal, mechanical, morphological properties and changes in these properties were investigated. The most suitable chemical blowing agent ratio was found to be 1.5 wt.% for PLA/PP and PLA/PA6 mixtures.
Perceptions of Patient Safety Among Middle Managers at an Operating Suite
Authors: Ramírez-Torres Carmen Amaia , Pedraz-Marcos Azucena , Maciá-Soler Maria Loreto , Rivera-Sanz Félix 
Published: June 2023

In 2008, the World Health Organization launched the Surgical Safety Checklist (SSC) to reduce the number of adverse events occurring at operating suites. The objective of this study was to explore middle managers’ experiences of patient safety culture, the meanings they attach to this culture, and their perceptions of one of its best-known strategies, the SSC. This study used a qualitative design involving structured interviews with the head of department, quality managers, and nursing supervisors at an operating suite. Eleven middle managers acknowledged that the SSC supports memory and instils accountability. Work overload was highlighted as the main barrier in SSC implementation, while feedback was identified as the main facilitator. We found significant differences in levels of understanding of patient safety culture among different types of middle managers and professions. Key aspects for promoting an effective, long-lasting patient safety culture were also identified.
Highly Effective Extraction of Gigantol from Dendrobium officinale Using the Ultrasonic-assisted Dry Grinding
Authors: Sun Yufeng , Zheng Xiuxia , Zhu Dong , Guan Dongyan , Fan Bei , Wang Fengzhong 
Published: June 2023

Background Dendrobium officinale is a valuable medicinal food plant in China, and its component gigantol has various biological activities. However, due to the low extraction rate of gigantol, its application in the functional food industry is limited. This research aimed to explore an effective gigantol extraction method. Results Ultrasonic-assisted dry grinding extraction of gigantol from D. officinale almost reached completion after 15 min. The extraction rate of gigantol reached 65%, which was higher than that of the conventional heating refluxing method of extraction. Most cells were broken, and particle size was approximately 410 nm when D. officinale was ground for 15 min. Conclusion The results indicated that ultrasonic-assisted dry grinding is a highly effective gigantol extraction method that is valuable for the efficient use and low-cost extraction of gigantol from the medicinal food plant D. officinale. Furthermore, this method will be beneficial for the future application of gigantol in the functional food industry.
Estimating the received solar irradiances by traditional vaulted roofs using optimized neural networks and transfer learning
Authors: Ayoub Mohammed 
Published: June 2023

Traditional vaulted roof-forms have long been utilized in hot-desert climate for better indoor environmental quality. Unprecedently, this research investigates the possible contribution of machine learning to estimate the received solar irradiances by those roofs, based on simulation-derived training and testing datasets, where two algorithms were used to reduce their higher-dimensionality. Then, four models of ordinary least-squares and artificial neural networks were developed. Their ability to accurately estimate solar irradiances was confirmed, with R2 of 95.599–98.794% and RMSE of 12.437–23.909 Wh/m2. Transfer Learning was also applied to pass the stored knowledge of the best-performing model into another one for estimating the performance of new roof-forms. The results demonstrated that transferred models could provide better estimations with R2 of 87.416–97.889% and RMSE of 79.300–13.971 Wh/m2, compared to un-transferred models. Machine learning shall redefine the practice of building performance, providing architects with flexibility to rapidly make informed decisions during the early design stages.
A study of the role of myeloid-derived growth factor on acute lung injury in vitro and In vivo
Authors: Gong Wenhui , Jiang Shi , Wang Jianbo , Gong Qian , Zhang Chengxin , Ge Shenglin 
Published: June 2023

Objectives: Bone marrow-derived mesenchymal stem cells (BMSCs) are considered to have potential clinical application value in the treatment of acute lung injury (ALI). Myeloid-derived growth factor (MYDGF) can promote the proliferation of stem cell. We hypothesized that MYDGF may play a role in reducing lung injury in vitro and in vivo through bone marrow mesenchymal stem cells. Methods: An in vitro model of lipopolysaccharide (LPS)(MLE-12) was established, which was divided into five groups: A: MLE-12; B: MLE-12+LPS; C: MLE-12+LPS + BMSCs; D: MLE-12+LPS + MYDGF; and E: MLE-12+LPS + BMSCs + MYDGF. A Cell Counting Kit-8 was used to detect the OD value. And an ALI model was constructed by inducing mice with a lipopolysaccharide. Forty male Balb/c mice were randomly divided into five groups: A control group; B: model group; C: LPS + BMSCs; D: LPS + MYDGF; E: LPS +BMSCs +MYDGF. Specimens were collected after 24 h. Hematoxylin-eosin (HE)-staining was performed on the tissue sections. The protein concentration in the alveolar lavage fluid was measure by bicinchoninic acid (BCA). The NF-κB, p-Akt, Bax, and Bcl-2 protein expression was detected through Western blotting, and Enzyme linked immunosorbent assay (ELISA) was used to measure the expression of serum interleukin-6, interleukin-10, and TNF-α. Results: Compared with the model group, BMSCs and MYDGF can alleviate the ALI induced by lipopolysaccharide in vitro and vivo ( p < .05). Conclusion: We found that the combined treatment effect of MYDGF and BMSCs was better than using MYDGF or BMSCs alone. We speculate that a pretreatment with MYDGF after ALI in mice may improve the survival and growth of transplanted MSCs, thereby improving the curative effect of cell transplantation.
Diagnosis, management and genetic analysis of a cat with primary copper hepatopathy
Authors: Recchia Benjamin K , Stokol Tracy , Goto-Koshino Yuko , Ohno Koichi , Miner Kayla DR 
Published: June 2023

Case summary A 2-year-old spayed female domestic longhair cat was presented for evaluation of chronic ocular discharge and occasional vomiting. While physical examination findings were consistent with an upper respiratory infection (URI), serum chemistry results revealed increased liver enzyme activities. Histopathologic examination of a liver biopsy identified substantial centrilobular accumulation of copper in hepatocytes – strongly suggestive of primary copper hepatopathy (PCH). Retrospective cytologic examination of a liver aspirate also identified copper aggregates in hepatocytes. After transitioning to a low-copper diet, 1 year of chelation therapy with D-penicillamine achieved normalization of liver enzyme activities and resolution of persistent ocular signs. Subsequently, a long-term regimen of zinc gluconate has been successfully managing the cat’s PCH for almost 3 years. Sanger sequencing of the cat’s ATP7B gene, which encodes a copper-transporting protein, revealed a novel, ‘likely pathogenic’, single nucleotide variation (c.3670t/a [p.Trp1224Arg]), for which the cat is heterozygous. Relevance and novel information Recommendations are described for the long-term clinical management of feline PCH – a previously attainable but unreported outcome – with considerations for mitigating the speculated oxidation-exacerbated ocular risks of concurrent URI. This report is the first to include identification of copper aggregates in a liver aspirate from a cat – evidence that liver aspirates from cats could be routinely examined for copper as is standard practice for those from dogs. The cat is also the first reported with PCH and a ‘likely pathogenic’ heterozygous ATP7B genotype, which suggests that normal ATP7B alleles could be recessive to or incompletely/co- dominant with deleterious ATP7B alleles in cats, as has been reported in other species.
The NLRP3 inflammasome: contributions to inflammation-related diseases
Authors: Chen Ying , Ye Xingyan , Escames Germaine , Lei Wangrui , Zhang Xin , Li Meng , Jing Tong , Yao Yu , Qiu Zhenye , Wang Zheng , Acuña-Castroviejo Darío , Yang Yang 
Published: June 2023

AbstractThe NOD-like receptor protein 3 (NLRP3) inflammasome is a protein complex that regulates innate immune responses by activating caspase-1 and the inflammatory cytokines interleukin (IL)-1β and IL-18. Multiple studies have demonstrated the importance of the NLRP3 inflammasome in the development of immune and inflammation-related diseases, including arthritis, Alzheimer’s disease, inflammatory bowel disease, and other autoimmune and autoinflammatory diseases. This review first explains the activation and regulatory mechanism of the NLRP3 inflammasome. Secondly, we focus on the role of the NLRP3 inflammasome in various inflammation-related diseases. Finally, we look forward to new methods for targeting the NLRP3 inflammasome to treat inflammation-related diseases, and provide new ideas for clinical treatment.
β-Ga2O3 MOSFETs electrical characteristic study of various etching depths grown on sapphire substrate by MOCVD
Authors: Lu Chan-Hung , Tarntair Fu-Gow , Kao Yu-Cheng , Tumilty Niall , Shieh Jia-Min , Hsu Shao-Hui , Hsiao Ching-Lien , Horng Ray-Hua 
Published: June 2023

Abstractβ-Ga2O3thin films with both a 45 nm Si-doped conductive epilayer and unintentionally doped epilayer were grown on c-plane sapphire substrate by metalorganic chemical vapor deposition. β-Ga2O3based metal–oxide–semiconductor field-effect transistors (MOSFETs) were fabricated with gate recess depths of 20 nm and 40 nm (it indicated gate depth with 70 nm and 50 nm, respective), respectively, and without said recessing process. The conductivity of β-Ga2O3epilayers was improved through low in situ doping using a tetraethoxysilane precursor to increase MOSFET forward current density. After recessing, MOSFET operation was transferred from depletion to enhanced mode. In this study, the maximum breakdown voltage of the recessed 40 nm transistor was 770 V. The etching depth of a recessed-gate device demonstrates its influence on device electrical performance.
Transcriptional programs are activated and microRNAs are repressed within minutes after mating in the Drosophila melanogaster female reproductive tract
Authors: Delbare Sofie Y. N. , Jain Asha M. , Clark Andrew G. , Wolfner Mariana F. 
Published: June 2023

AbstractBackgroundThe female reproductive tract is exposed directly to the male’s ejaculate, making it a hotspot for mating-induced responses. InDrosophila melanogaster, changes in the reproductive tract are essential to optimize fertility. Many changes occur within minutes after mating, but such early timepoints are absent from published RNA-seq studies. We measured transcript abundances using RNA-seq and microRNA-seq of reproductive tracts of unmated and mated females collected at 10–15 min post-mating. We further investigated whether early transcriptome changes in the female reproductive tract are influenced by inhibiting BMPs in secondary cells, a condition that depletes exosomes from the male’s ejaculate.ResultsWe identified 327 differentially expressed genes. These were mostly upregulated post-mating and have roles in tissue morphogenesis, wound healing, and metabolism. Differentially abundant microRNAs were mostly downregulated post-mating. We identified 130 predicted targets of these microRNAs among the differentially expressed genes. We saw no detectable effect of BMP inhibition in secondary cells on transcript levels in the female reproductive tract.ConclusionsOur results indicate that mating induces early changes in the female reproductive tract primarily through upregulation of target genes, rather than repression. The upregulation of certain target genes might be mediated by the mating-induced downregulation of microRNAs. Male-derived exosomes and other BMP-dependent products were not uniquely essential for this process. Differentially expressed genes and microRNAs provide candidates that can be further examined for their participation in the earliest alterations of the reproductive tract microenvironment.
Genome-wide identification and analysis of the WRKY gene family and low-temperature stress response in Prunus sibirica
Authors: Liu Quangang , Wang Shipeng , Wen Jiaxing , Chen Jianhua , Sun Yongqiang , Dong Shengjun 
Published: June 2023

Abstract
Background
WRKY transcription factors are a prominent gene family in plants, playing a crucial role in various biological processes including development, metabolism, defense, differentiation, and stress response. Although the WRKY gene family has been extensively studied and analysed in numerous plant species, research on Prunus sibirica’s WRKY genes (PsWRKY) remains lacking.

Results
This study analysed the basic physicochemical properties, phylogeny, gene structure, cis-acting elements, and Gene ontology (GO) annotation of PsWRKY gene family members using bioinformatics methods based on the whole-genome data of P. sibirica. In total, 55 WRKYs were identified in P. sibirica and were heterogeneously distributed on eight chromosomes. Based on the phylogenetic analysis, these WRKYs were classified into three major groups: Group I, Group II (II-a, II-b, II-c, II-d, II-e), and Group III. Members of different subfamilies have different cis-acting elements, conserved motifs, and intron-exon structures, indicating functional heterogeneity of the WRKY family. Prediction of subcellular localisation indicated that PsWRKYs were mainly located in the nucleus. Twenty pairs of duplicated genes were identified, and segmental duplication events may play an important role in PsWRKY gene family expansion. Analysis of the Ka/Ks ratio showed that the PsWRKY family’s homologous genes were primarily purified by selection. Additionally, GO annotation analysis showed that the WRKY gene family was mainly involved in responses to stimuli, immune system processes, and reproductive processes. Furthermore, quantitative real-time PCR (qRT-PCR) analysis showed that 23 PsWRKYs were highly expressed in one or more tissues (pistils and roots) and PsWRKYs showed specific expression patterns under different low-temperature stress conditions.

Conclusions
Our results provide a scientific basis for the further exploration and functional validation of WRKYs in P. sibirica.
Identification and immunological characterization of cuproptosis-related molecular clusters in ulcerative colitis
Authors: Pu Yunfei , Meng Xianzhi , Zou Zhichen 
Published: June 2023

Abstract
Background
Ulcerative colitis is one of the two main forms of inflammatory bowel disease. Cuproptosis is reported to be a novel mode of cell death.

Methods
We examined clusters of cuproptosis related genes and immune cell infiltration molecules in 86 ulcerative colitis samples from the GSE179285 dataset. We identified the differentially expressed genes according to the clustering method, and the performance of the SVM model, the random forest model, the generalized linear model, and the limit gradient enhancement model were compared, and then the optimal machine model was selected. To assess the accuracy of the learning predictions, the nomogram and the calibration curve and decision curve analyses showed that the subtypes of ulcerative colitis have been accurately predicted.

Results
Significant cuproptosis-related genes and immune response cells were detected between the ulcerative colitis and control groups. Two cuproptosis-associated molecular clusters were identified. Immune infiltration analysis indicated that different clusters exhibited significant heterogeneity. The immune scores for Cluster2 were elevated. Both the residual error and root mean square error of the random forest machine model had clinical significance. There was a clear correlation between the differentially expressed genes in cluster 2 and the response of immune cells. The nomogram and the calibration curve and decision curve analyses showed that the subtypes of ulcerative colitis had sufficient accuracy.

Conclusion
We examined the complex relationship between cuproptosis and ulcerative colitis in a systematic manner. To estimate the likelihood that each subtype of cuproptosis will occur in ulcerative colitis patients and their disease outcome, we developed a promising prediction model.
Maternal mental health screening and management by health workers in southwestern Uganda: a qualitative analysis of knowledge, practices, and challenges
Authors: Nakidde Gladys , Kumakech Edward , Mugisha John. F. 
Published: June 2023

Abstract
Background
Maternal mental health (MMH) problems, such as perinatal depression, maternal anxiety, suicide ideation and puerperal psychosis among others, have a significant impact on maternal morbidity and mortality, as well as the health and development of children. One in every four pregnant women and one in every five postpartum women in low-income countries, suffer from maternal mental health (MMH) problems. Despite this, MMH screening, diagnosis, and reporting remain scanty in Uganda. Consequently, this study aimed to investigate the knowledge, practices, and impediments that maternity care workers face when screening and managing women with maternal mental health disorders in health facilities in south-western Uganda.

Methods
In-depth interviews were conducted with 22 health-care professionals who work in maternity care departments in primary and tertiary healthcare facilities in southwestern Uganda to investigate their medical knowledge, clinical practices, and challenges related to the screening and management of maternal mental health problems. Using qualitative content analysis, distinct categories and subcategories were found.

Results
Medical staff especially midwives lacked specialized training in screening and managing women with maternal mental health problems They screened and managed MMH problems solely based on history and physical examination, and they referred nearly every mother displaying signs of mental illness because they felt ill-prepared to handle them. On the other hand, medical staff with some level of specialized training in mental health particularly staff working in mental health units, were more likely to use a mental health screening tool in addition to history and physical examination; and to treat any women exhibiting signs and symptoms of maternal mental problems without referring them. Lack of in-service training on maternal mental health, poorly coordinated referral systems, reluctance of mentally ill to visit medical facilities, scarcity of mental health specialists, and shortage of relevant medications were identified as the major challenges. Age, experience level, or gender had no effect on screening or management practices.

Conclusions
The results suggest that specialized training in mental health, and particularly maternal mental health, is essential for the effective screening and management of maternal mental health conditions in South Western Uganda.