bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of the Robotic-Assisted Digital Exoscope for Resection of Posterior Fossa Tumors in Adults: A Series of 45 Cases
Authors: Price Gabrielle , Schupper Alexander , Kalagara Roshini , Chennareddy Susmita , He Celestine , Zhang Jack Yin , Sudhir Sweta , Rentzeperis Frederika , Wanna George , Hadjipanayis Constantinos 
Published: July 2023

BACKGROUND AND OBJECTIVES:
Complete safe resection is the goal when pursuing surgical treatment for posterior fossa (PF) tumors. Efforts have led to the development of the exoscope that delineates tumors from non-neoplastic brain. This investigation aims to assess patient outcomes where PF tumor resection is performed with the exoscope by a retromastoid or suboccipital approach.

METHODS:
A retrospective analysis was conducted for patients with PF tumors who underwent exoscope resection from 2017 to 2022. Patient demographics, clinical, operative, and outcome findings were collected. Extent of resection studies were also performed. Associations between perioperative data, discharge disposition, progression-free survival (PFS), and overall survival (OS) were evaluated.

RESULTS:
A total of 45 patients (22 male patients) with a median age of 57 years were assessed. Eighteen (40%) and 27 patients (60%) were diagnosed with malignant and benign tumors, respectively. Tumor neurovascular involvement was found in 28 patients (62%). Twenty-four (53%) and 20 (44%) tumors formed in the cerebellum and cerebellopontine angle cistern, respectively. One tumor (2%) was found in the cervicomedullary junction. The mean extent of resection was 96.7% for benign and malignant tumors. The PFS and OS rate at 6 months (PFS6, OS6) was 89.7% and 95.5%, respectively. Neurological complications included sensory loss and motor deficit, with 11 patients reporting no postoperative symptoms. Of the neurological complications, 14 were temporary and 9 were permanent.

CONCLUSION:
The exoscope is an effective intraoperative visualization tool for delineating PF tumors. In our series, we achieved low postoperative tumor volumes and a high gross total resection rate.
SWI/SNF complexes are required for retinal pigmented epithelium differentiation and for the inhibition of cell proliferation and neural differentiation programs
Authors: Ovadia Shai , Cui Guizhong , Elkon Ran , Cohen-Gulkar Mazal , Zuk-Bar Nitay , Tuoc Tran , Jing Naihe , Ashery-Padan Ruth 
Published: July 2023

ABSTRACT
During embryonic development, tissue-specific transcription factors and chromatin remodelers function together to ensure gradual, coordinated differentiation of multiple lineages. Here, we define this regulatory interplay in the developing retinal pigmented epithelium (RPE), a neuroectodermal lineage essential for the development, function and maintenance of the adjacent retina. We present a high-resolution spatial transcriptomic atlas of the developing mouse RPE and the adjacent ocular mesenchyme obtained by geographical position sequencing (Geo-seq) of a single developmental stage of the eye that encompasses young and more mature ocular progenitors. These transcriptomic data, available online, reveal the key transcription factors and their gene regulatory networks during RPE and ocular mesenchyme differentiation. Moreover, conditional inactivation followed by Geo-seq revealed that this differentiation program is dependent on the activity of SWI/SNF complexes, shown here to control the expression and activity of RPE transcription factors and, at the same time, inhibit neural progenitor and cell proliferation genes. The findings reveal the roles of the SWI/SNF complexes in controlling the intersection between RPE and neural cell fates and the coupling of cell-cycle exit and differentiation.
Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration
Authors: Matsuda Kimiya , Hirayama Daiki , Hino Naoya , Kuno Sota , Sakaue-Sawano Asako , Miyawaki Atsushi , Matsuda Michiyuki , Terai Kenta 
Published: July 2023

ABSTRACT
The ErbB-family receptors play pivotal roles in the proliferation, migration and survival of epithelial cells. Because our knowledge on the ErbB-family receptors has been largely obtained by the exogenous application of their ligands, it remains unknown to what extent each of the ErbB members contributes to these outputs. We here knocked out each ErbB gene, various combinations of ErbB genes or all ErbB genes in Madin–Darby canine kidney cells to delineate the contribution of each gene. ERK1 and ERK2 (ERK1/2, also known as MAPK3 and MAPK1, respectively) activation waves during collective cell migration were mediated primarily by ErbB1 and secondarily by the ErbB2 and ErbB3 heterodimer. Either ErbB1 or the ErbB2 and ErbB3 complex was sufficient for the G1/S progression. The saturation cell density was markedly reduced in cells deficient in all ErbB proteins, but not in cells retaining only ErbB2, which cannot bind to ligands. Thus, a ligand-independent ErbB2 activity is sufficient for preventing apoptosis at high cell density. In short, systematic knockout of ErbB-family genes has delineated the roles of each ErbB receptor.
Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
Authors: Edgerton Heather D. , Mukherjee Soumya , Johansson Marnie , Bachant Jeff , Gardner Melissa K. , Clarke Duncan J. 
Published: July 2023

ABSTRACT
Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes that are not bioriented have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correction that locally loosens kinetochore–microtubule attachments to allow detachment of microtubules and fresh attempts at achieving biorientation. However, it is not known whether low tension recruits Aurora B to centromeres or, alternatively, whether low tension directly activates Aurora B already localized at centromeres. In this work, we experimentally induced low tension in metaphase Saccharomyces cerevisiae yeast cells, then monitored Ipl1 localization. We find low tension recruits Ipl1 to centromeres. Furthermore, low tension-induced Ipl1 recruitment depended on Bub1, which is known to provide a binding site for Ipl1. In contrast, Top2, which can also recruit Ipl1 to centromeres, was not required. Our results demonstrate cells are sensitive to low tension at centromeres and respond by actively recruiting Ip1l for error correction.
Cancer Stem Cells Persist Despite Cellular Damage, Emergence of the Refractory Cell Population
Authors: Nagae Ayumi , Miyoshi Norikatsu , Fujino Shiki , Horie Masafumi , Yachida Shinichi , Sasaki Masaru , Sekido Yuki , Hata Tsuyoshi , Hamabe Atsushi , Ogino Takayuki , Takahashi Hidekazu , Uemura Mamoru , Yamamoto Hirofumi , Doki Yuichiro , Eguchi Hidetoshi 
Published: July 2023

Abstract
Purpose
Cancer stem cells (CSCs) are responsible for chemotherapy resistance and have unique properties that protect them from chemotherapy. Investigating CSCs may help to identify the population that is more resistant to treatments, leading to recurrence. We evaluated persisting CSCs, emerging after chemotherapy that cause tumor recurrence.

Methods
Using human colorectal cancer organoids prepared from surgical specimens, we looked at changes in CSCs, the emergence and changes in the original population, which single-cell analysis identified.

Results
With regards to changes in cancer stem cell markers, CD44 showed low levels after 5-fluorouracil administration. Once the CD44-ve population was sorted and cultured, the CD44+ve population gradually emerged, and the CD44-ve population decreased. Compared with the CD44-ve population of an organoid parent, the CD44-ve population proliferated after chemotherapeutic agent stimulation. The CD44-ve population was derived from the CD44+ve population before chemotherapeutic agents. In addition, when the CD44 variants were evaluated, the CD44v9 population remained. In single-cell analysis, we found that POU5F1 was highly expressed in the CD44low population. Velocity analysis showed that the CD44-ve population was induced after chemotherapy and expressed POU5F1. POU5F1-EGFP-Casp9 transfected organoids resulted in the appearance of a CD44-ve population after administration of a chemotherapeutic reagent. Both in vivo and in vitro, the dimerizer administration inhibited tumor growth significantly.

Conclusions
POU5F1 is involved in chemotherapy resistance in relation to stemness. For the treatment against refractory tumors, such as the recurrence after chemotherapy, the treatment should target the emerging specific population such as CD44 (or CD44v9) and proliferative cancer cells.
How many university students perceive themselves to be overweight/obese and how many have eating disorders during remote education due to COVID-19? A cross-sectional study
Authors: Moya-Salazar Jeel , Goicochea-Palomino Eliane A. , Moya-Salazar María Jesús S. , Rojas-Zumaran Víctor , Contreras-Pulache Hans 
Published: July 2023

Background: The COVID-19 pandemic caused a health crisis worldwide that ended up affecting the daily lives of university students. Considering prevention strategies for contagion and decreased physical activity, altered perceptions of body image were seen that may negatively disrupt their eating habits. Methods: Our cross-sectional survey-based study (N=180) had the objective to determine the association between body image perception and the risk of eating disorders in university students during the pandemic. We surveyed male and female university students aged 18-35 years using the Montero Anatomical Models questionnaire and the Eating Attitude Test-26 (EAT-26) as instruments. Results: Most of the university students were female (67.8%) with an average age of 22.8±3.9 years. 51.1% perceived themselves as overweight and 53.9% were at low risk of an eating disorder. This mainly affected women (47.5%), the 21-30 age group (43.2%), and those who perceived themselves as underweight (58.9%) or obese (50%). Likewise, we found differences in body image perception between genders (p=0.009), and no association was found between body image and eating disorders (p=0.661). Conclusion: Even though most university students perceived themselves as overweight, they had a low risk of an eating disorder. Considering the influence that body image perception has on their lifestyle, it is necessary to promote healthy eating habits and self-esteem strategies to prevent the appearance of eating disorders due to body dissatisfaction in the context of the “new normal”.
Methodology of Moral and Religious Neo-Institutionalism for the National Education System in Russia (Consistency, Balance and Morality)
Authors: Privalov Nikolay , Bashmakova Natal'ya 
Published: July 2023

The subject of the article is the problem of the crisis of the Russian education system and, above all, the ideology and general methodology of teaching in the field of humanities. The purpose of the study is to formulate new principles as part of the author's methodology of training courses for higher education in the conditions of Russia's withdrawal from the Bologna process and the formation of a national educational system.
The modern education system is the result of many trends in the development of society — and above all secularization, the separation of science from religion and the development of the positivist nature of scientific knowledge. The crisis of modern education is a manifestation of the crisis of positivist science and the global crisis. Russia was the first in the world among non-Muslim countries to publicly declare the rejection of liberal principles of social structure. The paper proposes a new methodology — moral and religious neo-institutionalism, containing three main principles — system, balance and morality. The latest practical result of its implementation is the launch of the publication of the INFRA-M publishing house of a series of books "Russian University Textbook on Economics and Management" intended for master's degree (advanced level). Among them the textbook "Economic theory. Russian Economics" took the first place in the international academic course "Academus" in the nomination "Economics and Management".
Psychological features of patriotic education police officers of the Russian Federation
Authors: Dobrynina Oksana 
Published: July 2023

Within the framework of this article, we examined the main features of the education of patriotism among law enforcement officers from a psychological point of view. In particular, we analyzed the patterns that affect the development of positive, creative patriotism among law enforcement officers, which we noticed during educational work. In addition, we briefly described the key elements of the patterns of patriotism education for both future and current law enforcement officers, developed a number of recommendations for a more productive organization of educational activities.
The problem statement: serving in law enforcement agencies implies the presence of specific moral and mental qualities among police officers, which are designed to ensure firm stamina and an objective position of the individual. This is vital, since law enforcement requires endurance, confidence and courage. All these qualities, of course, reasonably place risks and priorities in the process of fulfilling military-political tasks while on combat duty.
The purpose of the study: to study the psychological characteristics of patriotic education of police officers of the Russian Federation
The research methods: dialectics, hermeneutics, synergetics, philosophical conceptology.
The results and key conclusions: in the course of the study, they were able to outline the likely difficulties and erroneous actions that may arise in the process of relaying the ideas of patriotism. This made it possible to provide a methodological substantiation of the need for the relevance of educating patriotism for the personality of a law enforcement officer: both in everyday life and in the course of service. A high level of patriotism provides counteraction to swindlers, terrorists, and makes it possible to carry out military and political tasks of national importance with high quality.
Towards the sustainable elimination of gambiense human African trypanosomiasis in Côte d’Ivoire using an integrated approach
Authors: Kaba Dramane , Koffi Mathurin , Kouakou Lingué , N’Gouan Emmanuel Kouassi , Djohan Vincent , Courtin Fabrice , N’Djetchi Martial Kassi , Coulibaly Bamoro , Adingra Guy Pacôme , Berté Djakaridja , Ta Bi Tra Dieudonné , Koné Minayégninrin , Traoré Barkissa Mélika , Sutherland Samuel A. , Crump Ronald E. , Huang Ching-I , Madan Jason , Bessell Paul R. , Barreaux Antoine , Solano Philippe , Crowley Emily H. , Rock Kat S. , Jamonneau Vincent 
Published: July 2023

Background
Human African trypanosomiasis is a parasitic disease caused by trypanosomes among which Trypanosoma brucei gambiense is responsible for a chronic form (gHAT) in West and Central Africa. Its elimination as a public health problem (EPHP) was targeted for 2020. Côte d’Ivoire was one of the first countries to be validated by WHO in 2020 and this was particularly challenging as the country still reported around a hundred cases a year in the early 2000s. This article describes the strategies implemented including a mathematical model to evaluate the reporting results and infer progress towards sustainable elimination.

Methods
The control methods used combined both exhaustive and targeted medical screening strategies including the follow-up of seropositive subjects– considered as potential asymptomatic carriers to diagnose and treat cases– as well as vector control to reduce the risk of transmission in the most at-risk areas. A mechanistic model was used to estimate the number of underlying infections and the probability of elimination of transmission (EoT) was met between 2000–2021 in two endemic and two hypo-endemic health districts.

Results
Between 2015 and 2019, nine gHAT cases were detected in the two endemic health districts of Bouaflé and Sinfra in which the number of cases/10,000 inhabitants was far below 1, a necessary condition for validating EPHP. Modelling estimated a slow but steady decline in transmission across the health districts, bolstered in the two endemic health districts by the introduction of vector control. The decrease in underlying transmission in all health districts corresponds to a high probability that EoT has already occurred in Côte d’Ivoire.

Conclusion
This success was achieved through a multi-stakeholder and multidisciplinary one health approach where research has played a major role in adapting tools and strategies to this large epidemiological transition to a very low prevalence. This integrated approach will need to continue to reach the verification of EoT in Côte d’Ivoire targeted by 2025.
Risk prediction models for mortality and readmission in patients with acute heart failure: A protocol for systematic review, critical appraisal, and meta-analysis
Authors: Zhang Xuecheng , Zhou Kehua , You Liangzhen , Zhang Jingjing , Chen Ying , Dai Hengheng , Wan Siqi , Guan Zhiyue , Hu Mingzhi , Kang Jing , Liu Yan , Shang Hongcai 
Published: July 2023

Introduction
A considerable number of risk models, which predict outcomes in mortality and readmission rates, have been developed for patients with acute heart failure (AHF) to help stratify patients by risk level, improve decision making, and save medical resources. However, some models exist in a clinically useful manner such as risk scores or online calculators, while others are not, providing only limited information that prevents clinicians and patients from using them. The reported performance of some models varied greatly when predicting at multiple time points and being validated in different cohorts, which causes model users uncertainty about the predictive accuracy of these models. The foregoing leads to users facing difficulties in the selection of prediction models, and even sometimes being reluctant to utilize models. Therefore, a systematic review to assess the performance at multiple time points, applicability, and clinical impact of extant prediction models for mortality and readmission in AHF patients is essential. It may facilitate the selection of models for clinical implementation.

Method and analysis
Four databases will be searched from their inception onwards. Multivariable prognostic models for mortality and/or readmission in AHF patients will be eligible for review. Characteristics and the clinical impact of included models will be summarized qualitatively and quantitatively, and models with clinical utility will be compared with those without. Predictive performance measures of included models with an analogous clinical outcome appraised repeatedly, will be compared and synthesized by a meta-analysis. Meta-analysis of validation studies for a common prediction model at the same time point will also be performed. We will also provide an overview of critical appraisal of the risk of bias, applicability, and reporting transparency of included studies using the PROBAST tool and TRIPOD statement.

Systematic review registration
PROSPERO registration number CRD42021256416.
Clinical features and outcomes of hospitalised patients with COVID-19 and Parkinsonian disorders: A multicentre UK-based study
Authors: Sorrell Lexy , Leta Valentina , Barnett Anton , Stevens Kara , King Angela , Inches Jemma , Kobylecki Christopher , Walker Richard , Chaudhuri K. Ray , Martin Hannah , Rideout Jane , Sneyd J. Robert , Campbell Sarah , Carroll Camille ,   on behalf of the COVID-19 PD UK study team
Published: July 2023

BackgroundParkinson’s disease has been identified as a risk factor for severe Coronavirus disease 2019 (COVID-19) outcomes. However, whether the significant high risk of death from COVID-19 in people with Parkinson’s disease is specific to the disease itself or driven by other concomitant and known risk factors such as comorbidities, age, and frailty remains unclear.ObjectiveTo investigate clinical profiles and outcomes of people with Parkinson’s disease and atypical parkinsonian syndromes who tested positive for COVID-19 in the hospital setting in a multicentre UK-based study.MethodsA retrospective cohort study of Parkinson’s disease patients with a positive SARS-CoV-2 test admitted to hospital between February 2020 and July 2021. An online survey was used to collect data from clinical care records, recording patient, Parkinson’s disease and COVID-19 characteristics. Associations with time-to-mortality and severe outcomes were analysed using either the Cox proportional hazards model or logistic regression models, as appropriate.ResultsData from 552 admissions were collected: 365 (66%) male; median (inter-quartile range) age 80 (74–85) years. The 34-day all-cause mortality rate was 38.4%; male sex, increased age and frailty, Parkinson’s dementia syndrome, requirement for respiratory support and no vaccination were associated with increased mortality risk. Community-acquired COVID-19 and co-morbid chronic neurological disorder were associated with increased odds of requiring respiratory support. Hospital-acquired COVID-19 and delirium were associated with requiring an increase in care level post-discharge.ConclusionsThis first, multicentre, UK-based study on people with Parkinson’s disease or atypical parkinsonian syndromes, hospitalised with COVID-19, adds and expands previous findings on clinical profiles and outcomes in this population.
A longitudinal analysis of selective motor control during gait in individuals with cerebral palsy and the relation to gait deviations
Authors: Sorek Gilad , Goudriaan Marije , Schurr Itai , Schless Simon-Henri 
Published: July 2023

Objective
To investigate longitudinal changes in selective motor control during gait (SMCg) in individuals with cerebral palsy (CP), and to assess if they are related to changes in gait deviations.

Method
Twenty-three children/adolescents with spastic CP (mean ± SD age = 9.0±2.5 years) and two 3D gait assessments (separated by 590±202 days) with no interim surgical intervention, were included. SMCg was assessed using muscle synergy analysis to determine the dynamic motor control index (walk-DMC). Gait deviation was assessed using the Gait profile score (GPS) and Gait variable scores (GVS).

Results
There were no mean changes in walk-DMC score, GPS or GVS between assessments. However, changes in walk-DMC scores in the more involved leg related to changes in hip flexion-extension and hip internal-external GVS (rp = -0.56; p = 0.017 and rp = 0.65; p = 0.004, respectively).

Conclusions
On average, there were no significant longitudinal changes in SMCg. However, there was considerable variability between individuals, which may relate to changes in hip joint kinematics. This suggests that a combination of neural capacity and biomechanical factors influence lower limb muscle co-activation in individuals with CP, with a potential important role for the hip muscles. These findings highlight the importance of taking an individualized approach when evaluating SMCg in individuals with CP.