bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drug-resistant EGFR lung cancer mutations promote tumor growth by stabilizing interfaces in ligand-free signaling-competent EGFR oligomers
Authors: Martin-Fernandez Marisa , Gervasio Francesco , Rolfe Daniel , Iyer R. Sumanth , Needham Sarah , Roberts Selene , Zanetti-Domingues Laura , Davis Benjamin , Clarke David , Fruhwirth Gilbert , Man Rico , Galdadas Ioannis , Parker Peter 
Published: July 2023

Abstract
Epidermal growth factor receptor (EGFR) is frequently mutated in non-small cell lung cancer (NSCLC) where it has been successfully targeted with tyrosine kinase inhibitors, but these are eventually overcome by drug resistance. Overcoming this therapeutic challenge has been hindered by the poor understanding of how wild type (WT)-EGFR elicits ligand-independent signals essential for cell homeostasis, which are then hijacked by cancer mutations. Ligand-independent signaling cannot be explained by the autoinhibited ligand-free EGFR dimer and oligomer structures so far available. Here we show that EGFR achieves ligand-independent phosphorylation by assembling dimer conformers into obligate ligand-free hetero-conformational oligomers. We reveal the structure of these ligand-free oligomers and their mechanisms of assembly. We also show how these mechanisms are exploited by drug-resistant T766M and Ex20ins EGFR mutants, revealing oncogenic functions for hitherto orphan transmembrane and kinase interfaces, and for the ectodomain tethered conformation of EGFR. Our findings provide a framework for future drug discovery directed at tackling EGFR mutations in cancer by disabling oligomer-assembling interactions.
Osteoporosis self-assessment tool for Asians as a risk assessment tool of airflow limitation in Japanese post-menopausal women
Authors: Watanabe Kazuhiko , Omori Hisamitsu , Onoue Ayumi , Kubota Kenichi , Yoshida Minoru , Katoh Takahiko 
Published: July 2023

Abstract
Reduced respiratory function is associated with low bone mineral density and increased fracture risk. Therefore, we assessed the association between the osteoporosis self-assessment tool for Asians (OSTA) and airflow limitation (AL) in post-menopausal Japanese women. This cross-sectional study included 1,580 patients who underwent a comprehensive health examination using spirometry and dual-energy X-ray absorptiometry. The OSTA was calculated by subtracting the age in years from the body weight in kilograms, and the result was multiplied by 0.2. Furthermore, the OSTA risk level was defined as low (> -1), moderate (-4 to -1), or high (< -4); AL was defined as forced expiratory volume in 1 s/forced vital capacity (FEV1/FVC) < 0.7. Prevalence of AL was significantly higher in the high OSTA group (15.3%) than in the low OSTA group (3.1%). The OSTA was independently associated with FEV1/FVC. Moreover, in logistic regression models adjusted for smoking status, alcohol consumption, current use of medication for diabetes, hyperglycemia, rheumatoid arthritis, second-hand smoke, and removal of ovary, the risk of AL (adjusted odds ratio: 5.48; 95% confidence interval: 2.90–10.37) was significantly higher in participants with OSTA high-risk than in those with low-risk. Thus, OSTA high-risk indicates the presence of AL in Japanese post-menopausal women.
Transplantation of Healthy Mitochondria into Rat Renal Proximal Tubular Cells Reduces Colistin- Induced Mitochondrial Dysfunction and Cellular Damage: An In Vitro Study
Authors: Arjmand Abdollah , Mohammadabadi Maryam , Faizi Mehrdad , Fakhri Amir , Jamali Zhaleh , Salimi Ahmad , Pourahmad Jalal 
Published: July 2023

Abstract
Background Kidney damage caused by colistin (polymyxin E) can bring about a decrease in creatinine clearance, potential proteinuria, cylindruria and oliguria in treated patients. It is therefore imperative to develop a new therapeutic strategy for reducing kidney damage after treatment with colistin. Mitochondrial damage is one of contributing factors in colistin-induced nephrotoxicity. Given the therapeutic benefits of mitochondrial transplantation by exogenous healthy mitochondria, we hypothesized that this strategy would be capable of ameliorating renal proximal tubular cells damage following exposure with colistin.Methods For this purpose, we isolated rat renal proximal tubular cells (RPTCs) form kidney and exposed them with toxic concertation of colistin with/without rat healthy isolated mitochondria for 4 hours. Cellular parameters such as lactate dehydrogenase (LDH), reactive oxygen species (ROS) formation, mitochondrial membrane potential (MMP), caspase 3 activation, lysosomal damage, glutathione and ATP content were measured.Results The results showed that administration of isolated mitochondria could improve colistin-induced nephrotoxicity and reduce mitochondrial dysfunction. Exogenous mitochondria reduced the activity of LDH, production of ROS, ATP and GSH depletion, loss of MMP, lysosomal damages and cell death.Conclusion To the best of our knowledge, these results provide the first direct experimental evidence that mitochondrial transplantation is capable of ameliorating cellular damage following treatment with colistin. These findings support that mitochondrial transplantation can be a promising therapeutic strategy for colistin-associated mitochondrial dysfunction and kidney damage.
Influence of tool flank wear with considering tool edge radius on instantaneous uncut chip thickness and cutting force in micro-end milling
Authors: Gao Shuaishuai , Duan Xianyin , Zhu Kunpeng , Zhang Yu 
Published: July 2023

Abstract

Accurate theoretical analysis and modeling of instantaneous uncut chip thickness (IUCT) play a crucial role in the cutting force prediction of the micro-milling process. Tool wear and tool runout have a significant influence on the IUCT and become key factors to be considered. An IUCT model covering the influence of tool runout and tool flank wear with considering tool edge radius is proposed. Based on this the prediction model of the cutting force is constructed in the micro-end milling process. Firstly, an actual tool radius model considering tool flank wear and tool edge radius is analyzed. The IUCT model is established by constructing the trochoidal trajectories of the current cutting edge and all cutting edges in the previous cycle with considering tool flank wear and tool runout. Moreover, tool flank wear and tool runout are considered in the determination of cutter-workpiece engagement and the calibration of cutting force coefficients. Effectiveness of the established cutting force model is verified by micro milling experiments and statistic analysis. Results show that tool flank wear has a significant effect on the cutting forces of the three directions, and the influence of tool flank wear on the IUCT is small in a certain range. This work for predicting the IUCT and cutting force sheds new light on revealing various derived physical phenomena such as deformation, heat, and stress in the micro-cutting process.
Nutritional comparison and evaluation of two macroalgae, Sargassum ilicifulium and Padina australis, as partial substitution of fish meal in practical diets of Asian sea bass juvenile (Lates calcarifer)
Authors: Morshedi Vahid , Gamoori Reza , Yilmaz Sevdan , Hamedi Shirin , Ghasemi Ahmad , Shapawi Rossita 
Published: July 2023

Abstract
In the present study, Asian seabass (Lates calcarifer) with an initial mean body weight of 29.0 ± 1.0 g (in your materials and methods -31.0 g- which one is correct?) has been used as a model organism to establish the effects of dietary of Sargassum ilicifulium and Padina australis, as partial substitution of fish meal on growth performance, body composition, hemato-immunlogical response, digestive enzymes and immune and growth-related genes. Three diets were formulated in which fish meal (FM) was gradually replaced by S. ilicifolium (SIM) and P. australis meal (PAM) at 6% (S6 & P6, respectively) whereas in the control diet (C), FM was not replaced with macroalgae. Asian seabass juveniles were fed for six weeks with experimental diets. At the end of the experiment, growth and feed utilization parameters in P6 group were significantly higher than those of the control group (P < 0.05). The P. australis dietary supplementation showed differences significantly in RBC, Hb, Hct, WBC to write in full during 1st mention, lymphocytes, neutrophils, and cholesterol contents (P < 0.05). It was observed that the levels of total protein and triglyceride in fish fed S. ilicifulium diet significantly increased compared to the other groups (P < 0.05). The skin mucus total immunoglobulin (Ig) and mucus lysozyme in the group fed on P6 were significantly higher than the control group (P < 0.05). The serum total Ig in the group fed on S6 was significantly higher with respect to the control group (P < 0.05). No significant differences were recorded in the contents of ACH50 in full during 1st. mention and serum lysozyme among all treatment groups (P > 0.05). There was no significant difference in the contents of protease, lipase, and amylase among all treatments (P > 0.05). At the end of the experiment, the insulin-like growth factor I (IGF-I), lysozyme (LZ), and interleukin-1β (IL-1β) in P6 were significantly higher than other treatments (P < 0.05). The results obtained in the present study showed that P. australis as a partial replacement for fishmeal in the diet of Asian sea bass juveniles is beneficial for its immune system and growth performance stimulatory effects.
Rindler Trajectories in Cloud of strings in 3rd order Lovelock gravity
Authors: Shahzad M Umair , Sadaf Aneela 
Published: July 2023

Abstract
This paper studies the Rindler trajectories in the Cloud of strings in 3rd-order Lovelock gravity. According to the generalization of the Letaw-Frenet equations for curved spacetime (ST), the trajectory will continue to accelerate linearly and uniformly throughout its motion. The ST of the Cloud of strings in 3rd-order Lovelock gravity, a boundary is established on the bound of the accelerated magnitude |a| for radially inward traveling trajectories in the expression of the BH mass M which is represented by |a| ≤ 2/(√105 M). For a certain selection of asymptotic initial data h, the linearly uniformly accelerated trajectory always enters the BH for acceleration |a| greater than the bound value. To study the bound value by |a|, the radial linearly uniformly accelerated trajectory can only travel to infinity within a small radius or the distance of the closest approach. However, it is observed that when the bound |a| = 2/(√105M) is saturated, and this distance approaches its lowest value of rb = (b+2)√ 7 √ 5M 4 √ b+1 , r > rs. We also demonstrate that the value of the acceleration has a limited constraint, there is always an extension of the closest approach rb > (b+2) √ 7 √ 5M 6 √ b+1 for |a| ≤ B(M, h), for each set of finite asymptotic initial data h.
Enhancing Semantic Segmentation throughCycle-consistent Label Propagation in Video
Authors: Addanki Veerababu , Yerramreddy Dhanvanth Reddy , Durgapu Sathvik , Boddu Sasi Sai Nadh , Durgapu Vyshnav 
Published: July 2023

Abstract
To perform semantic image segmentation using deep learning models, a significant quantity of data and meticulous manual annotation is necessary , and the process consumes a lot of resources, including time and money. To resolve such issues, we introduce a unique label propagation method that utilizes cycle consistency across time to propagate labels over longer time horizons with higher accuracy. Additionally, we acknowledge that dense pixel annotation is a noisy process , whether performed manually or automatically. To address this, we present a principled approach that accounts for label uncertainty when training with labels from multiple noisy labeling processes. We introduce two new approaches; Warp-Refine Propagation and Uncertainty- Aware Training, for improving label propagation and handling noisy labels, respectively, and support the process with quantitative and qualitative evaluations and theoretical justification. Our contributions are validated on the Cityscapes and ApolloScape datasets, where we achieve encouraging results. In later endeavors, the aim should be to expand such approaches to include other noisy augmentation processes like image-based rendering methods, thanks to the noisy label learning approach.
Research on the Construction of Ecological Landscape Environment of Guanzhong Village under the Strategy of Rural Revitalization
Authors: Cao Jun , Pengjie HAN , ZHANG Wei , ZHAO Guangshe 
Published: July 2023

Abstract
In the construction of the ecological landscape of Guanzhong villages, the traditional construction methods lack standards that meet the current ecological needs, making the construction incomplete and the ecological benefits cannot meet the development needs of the village. To this end, a study on the construction of the ecological landscape environment of Guanzhong villages under the rural revitalization strategy is proposed. According to the national ecological construction standards, establish the village ecological landscape environment construction standards, consider the future development of the village, determine the ecological landscape environment construction principles, and on this basis, use the ecological construction in the city as a reference to construct the ecological landscape environment. The experimental results show that the ecological landscape environment construction method of Guanzhong Village under the designed rural revitalization strategy is more comprehensive, and the four-dimensional score is higher.
Acute Oral Toxicity, Antioxidant Activity and Molecular Docking Study
of 2-(4-Bromo-phenoxy)-N-[6-chloro-4-(4-chlorophenyl)-3-cyano-4H-chromen-
2-yl]-acetamide
Authors: Chauhan Divya , Agrawal Anurag , Sahu Jagdish K. , Kumar Sushil 
Published: July 2023

Background:
Several studies have been conducted on 4-H chromene compounds because
of their intriguing pharmacological and biological properties. Various new natural compounds having
a chromene foundation have been reported over the past 20 years.

Objective:
In the present study, we reported the acute oral toxicity, antioxidant activity, and molecular
docking study of the most active 4H-chromene derivative2-(4-Bromo-phenoxy)-N-[6-chloro-4-(4-
chlorophenyl)-3-cyano-4H-chromen-2-yl]-acetamide (A9).

Method:
The acute oral toxicity was carried out as per OECD 423 guidelines. For investigating the antioxidant
activity, various biochemical parameters in colon tissue like SOD, CAT, MDA, PC and GSH
and also enzyme levels, such as ALT, AST, ALP, and LDH, were measured in this experiment.

Results:
Acute oral toxicity study indicated that the A9 ligand was found to be safer in animals. Additionally,
the A9 ligand had significant antioxidant properties at various doses and was not found to be
harmful to the liver. Due to its stronger binding energy and the appropriate interactions that induce inhibition,
the A9 ligand's antioxidant function was also validated by additional molecular docking research.

Conclusion:
This compound can be exploited as a lead molecule in further research.
Phytochemical analysis of Different extract of Hyptis suaveolens(L.)Poit
Authors: Gaikwad Saurav , Deore Sonali , Adsare Akanksha 
Published: July 2023

Aromatic plants are the basin of secondary metabolites that are accountable for the plants medicinal efficacy and major origin of aromatic compound and essential oils. Now days due to richness of bioactive elements in plants they play a crucial role in production of enormously increasing herbal product. In the present study Hyptis suaveolens (L.)Poit from Mahodari ghat region were analysed for secondary metabolites by using plant parts (roots, leaves, stem and seed) and by using range of solvent from polar to non-polar. Many of different phytochemical Glycosides, Terpenoids, tannins, Flavonoids, alkaloids, coumarins, phlobatannins and phytosterols were found in methanolic extract of leaf and stem, seeds. While extract of hexane, Petroleum ether and chloroform exhibits presence of some of them. Obtained results show importance of biological active molecules for pharmaceutical and cosmetic industries.
Keywords Phytochemical, Secondary metabolites, bioactive compound
The Spatial Ecological Environment Design of the Waterfront Public Areas of Rural Rivers
Authors: Ge Zhengxi , Xin Zilong 
Published: July 2023

Abstract
To revitalise established urban areas in the context of stock planning, the river surges in established urban areas play an irreplaceable role. In recent years, the water systems of rivers and surges in established urban areas of many international metropolises have undergone transformation and regeneration. The formerly hardened river banks have begun to be transformed into post-industrial places of integrated residence, leisure, and work, reshaping the ecological landscape and the functions of leisure activities of the rivers and urban spaces. By selecting typical representative cases for analysis and research, this paper determines the research angle and conducts a comparative study of waterfront open space in small and medium-sized cities and large cities from the perspectives of spatial layout, functional structure, ecological restoration, and artistic style, summarises the characteristics and current problems of waterfront open space in small and medium-sized cities at the present stage, and takes the problem as a guide, combining urban design, place theory and other theoretical studies as a guide. Planning and design strategy for waterfront open space in small and medium-sized cities is proposed. This paper selects representative waterfront open spaces and open waterfront spaces for field research. It uses the waterfront open space planning strategy proposed in the paper as a guide to provide a basis for waterfront open space planning and design and, at the same time, selects typical lots for optimal design to verify the implementation ability of the strategy. This paper digs into the rich historical, and geographical information contained in the sample villages in each area summarises the site selection characteristics of traditional villages in Hebei at the mesoscopic level between different areas and analyses the characteristics of the overall layout of villages dominated by natural factors such as mountains, hills and plains, and human factors such as ancient defile post roads, military defences, industries and temples. The spatial skeleton structure of the single-axis type, multi-axis type, organic network type, regular network type, and the fortress-wall enclosed village is extracted. The characteristics of its subtypes are summarised with sample cases. The elemental composition of village centres and boundaries is summarised.
Examining the Adoption of Lean Manufacturing in Lima Companies: An Exploratory Study of University Theses
Authors: Presentación Ricardo Villena , Perfecto Heriberto Reyes , Ruiz Roger Orlando Lujan , Andia Valencia Walter 
Published: July 2023

Purpose: The objective of this research was to know the results of the adoption of lean manufacturing in companies in Lima, which were reported in theses for university degrees.

Theoretical framework: The adoption of lean manufacturing is challenging and complex and requires specialized process management due to the various barriers and drivers to adoption.

Design/methodology/approach: The study was conducted using an exploratory and descriptive methodology that consisted of reviewing university theses related to lean manufacturing. Subsequently, companies were identified and contacted by telephone, applying questionnaires with direct questions about adoption results.

Findings: The results were obtained from the review and comparison of university repositories, SUNAT (Superintendencia de administración tributaria) and telephone calls. Descriptive analyses were performed to evaluate the results, which provided insight into the application of the methodology.

Research, Practical & Social implications: It is suggested for future research to explore the adoptions of lean manufacturing proposed in articles of indexed journals to learn about their experiences and adapt them to the national reality.

Originality/value: The results indicate that most of the university theses reviewed only propose the adoption of lean manufacturing and have not been implemented due to the complexity and sustainability of the methodology.
Improving drinking water consumer confidence reports: Applying user-centered design
Authors: Fox Mary , Agarwalla Vidisha , Iuliano Kayla  R. , Munroe J.  Harry , Nachman Keeve  E. , Burke Thomas  A. 
Published: July 2023

The US Environmental Protection Agency is revising its policy on drinking water quality reports for consumers. These reports are intended to enhance the public’s “right to know” and to spur action to protect and promote safe water. However, these reports are known to be highly technical and difficult to access compromising their communication value. This study engaged a focus group to gather evidence on how these reports can be improved. We applied user-centered design principles to understand public drinking water consumer information needs and preferences and to develop new communication tools and methods. Through a set of in-depth interviews, we learned that most participants were unaware of the report until introduced to it during the study. The focus group participants voiced preferences for: better ways to convey technical information; more health information; a clearer understanding of costs and billing; and neighborhood or household level water quality information. Following the interviews, we convened two rounds of small group meetings to create new report designs and to review and refine the designs. The focus group developed a one-page summary statement, water contaminant trend charts, an interactive map, and other recommendations on ways to improve dissemination of the report. The project results, focus group recommendations and designs were submitted to the US Environmental Protection Agency for consideration as the policy for these reports is finalized. We believe these findings provide valuable insights into water quality communication challenges that are widely applicable and will be informative for water utilities as they prepare future reports.