bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: Nephrotic syndrome as the primary manifestation of Alport syndrome in a Chinese pediatric patient
Authors: Song Yue , Li Yifei , Lu Liqun , Yang Changqiang , Lu Jing 
Published: January 2025

BackgroundAlport syndrome (AS) is a genetically heterogeneous disorder resulting from variants in genes coding for the alpha-3/4/5 chains of Collagen IV, leading to defective basement membranes in the kidney, cochlea, and eye. The clinical manifestations of AS vary in patients. Cases of childhood AS caused by COL4A3 presenting primarily with nephrotic syndrome (NS) are rarely reported. Here, we report a pediatric case presenting initially with NS attributed to AS caused by COL4A3.Case presentationAn 11-year-old boy presented with hematuria and nephrotic range proteinuria. After excluding secondary causes, primary NS was considered. He was administered with prednisone (60 mg/day). The patient had not responded to treatment by the end of 4 weeks, so he was diagnosed with steroid-resistant NS. A renal biopsy showed granular and vacuolar degeneration of renal tubular epithelial cells, multifocal foam cell infiltration in the renal interstitium, and immunofluorescence indicated the absence of α3, α4, and α5 expression in the glomerular and tubular basement membrane, while Bowman's capsule expression was normal. Electron microscopy ultrastructural suggested variable basement membrane thickness, and partial tearing and web-like structures. Genetic testing revealed a heterozygous COL4A3 missense mutation c.3210 (exon 37)G&gt;A(NM:000091). These findings are consistent with the diagnosis of AS. Prednisone was gradually tapered and enalapril maleate was initiated.ConclusionWe have described a pediatric case of AS featuring NS as its primary manifestation. It is important to consider AS to be a diagnosis or differential diagnosis in patients who have NS with hematuria or steroid resistance.
Safety of combined drug use in patients with cardiovascular and cerebrovascular diseases: an analysis based on the spontaneous reporting database of adverse drug reactions in Hubei Province
Authors: Wang Jia , Zhao Yuhang , Chen Zherui , Huang Rui 
Published: January 2025

ObjectiveThere is a lack of studies investigating the safety of combination regimens specifically for cardiovascular and cerebrovascular diseases. This study aimed to evaluate the safety of combination drugs for cardiovascular and cerebrovascular diseases using real-world data.MethodsWe analyzed adverse drug reaction data received by the Hubei Adverse Drug Reaction Center from the first quarter of 2014 to the fourth quarter of 2022. The safety of combined drugs for cardiovascular and cerebrovascular diseases in different people was assessed using the association rule method and Ω shrinkage measurement.ResultsA total of 53,038 reports were included in this study, revealing 9 signals of adverse reactions caused by combination drugs. The strongest signal found in this study was jaundice caused by the combination of amlodipine and atorvastatin (Ω 0.025:3.08, lift: 1116.69, conviction: 1.75). Additionally, the combination of aspirin with other drugs was associated with hemorrhaging in various organs. Female patients showed a cold signal when taking vitamin C and vitamin B6 together compared to male patients (Ω 0.025:0.89, lift: 7.15, conviction: 1.12). Patients under 60 years old had a palpitations signal when combining eritrea bei sha Tanzania and felodipine (Ω 0.025:0.41, lift: 14.65, conviction: 3.8), and an erythema signal when combining nifedipine (Ω 0.025:0.23, lift: 8.17, conviction: 1.077).ConclusionAmong the 9 signals identified in this study, 4 were off-label adverse drug reactions that require further clinical research for exploration and confirmation, in order to provide more scientifically informed drug labeling. Five adverse events associated with aspirin-induced bleeding were identified. Notably, different adverse drug reactions were observed in different populations, suggesting the need for future studies to expedite the development of personalized medicine.
Oral administration of ethanol extract from Gynura procumbens stems corrects kidney injury and renal anemia in chronic kidney disease
Authors: Li Ting-Ting , Wen Li-Ying , Meng Sha-Sha , Li Yu-Sang , Tang He-Bin 
Published: January 2025

BackgroundGynura procumbens (Lour.) Merr. is a plant used in traditional Chinese medicine that reduces hepatotoxicity, relieves kidney discomfort, and has anti-inflammatory and antioxidant properties. MethodsWe aimed to explore the mechanisms underlying the therapeutic effects of an ethanol extract from G. procumbens stems (EEGS) and selected metabolites on kidney injury and renal anemia associated with chronic kidney disease (CKD). An adenine-induced rat CKD model was used to elucidate the effective mechanism of EEGS and selected metabolites to correct renal anemia.ResultsThe results showed that treatment with EEGS reversed abnormal changes in the blood indicators, including hemoglobin, red blood cells, serum erythropoietin (EPO), and creatinine levels. Moreover, EEGS inhibited xanthine oxidase (XOD) activity in vitro, significantly inhibited liver XOD activity, and reduced intrahepatic inflammatory infiltration. Analysis of the pathological changes revealed that EEGS treatments resulted in reduced renal tubular apoptosis, decreased a number of crystals, a narrowed tubular lumen, and attenuated tubular fibrosis. Immunohistochemical staining revealed that EEGS significantly ameliorated the adenine-induced abnormal changes in the expression of related proteins, including β-catenin, COX-2, HIF-2α, and EPO, in the rat kidney tissues. Among the selected EEGS metabolites, the combined effect of chlorogenic acid and trans-p-coumaric acid was superior to that of either compound alone.ConclusionThese results suggest that EEGS and selected metabolites can effectively correct renal anemia in CKD rats by inhibiting XOD activity, reducing inflammation, and alleviating renal injury.
Normative and limit values of speed, endurance and power tests results of young football players
Authors: Nowak Michał , Szymanek-Pilarczyk Marta , Stolarczyk Artur , Oleksy Łukasz , Muracki Jarosław , Wąsik Jacek 
Published: January 2025

IntroductionThis study aimed to assess the development of speed, endurance and power in young football players and to create percentile charts and tables for standardized assessment.MethodsCross-sectional data were collected from 495 male players aged 12–16 years at RKS Raków Częstochowa Academy in 2018–2022. Players participated in a systematic training in which running time 5 m, 10 m, 30 m, lower limb power (standing long jump), and Maximum Aerobic Speed (MAS) were measured using the 30–15 Intermittent Fitness Test. All tests were performed under constant environmental conditions by qualified personnel. Statistical analysis included ANOVA and percentile distribution for P3, P10, P25, P50, P75, P90, P97.ResultsResults indicated that the most significant improvements occurred between the ages of 13 and 14, with increased speed over all distances and a significant increase in power. Percentile tables were developed, highlighting improvements in speed 5 m: 0.087–0.126 s; 10 m 0.162–0.215 s; 30 m: 0.438–0.719 s and power in the long jump test: 31–48 cm. Improvements in MAS ranged from 0.3 to 0.6 m/s across the percentiles.DiscussionThe results highlight the need for individual training programs tailored to the biological maturity of players. The developed percentile charts and tables offer a valuable tool for coaches and sports scientists to monitor progress, optimize training loads, and minimize the risk of injury, providing a frame of reference for assessing the physical development of young soccer players. Future research should focus on extending these charts and tables to other age groups and genders to refine training methodologies further.
Morphology, phylogeography, phylogeny, and taxonomy of Cyclorhiza (Apiaceae)
Authors: Song Bo-Ni , Aou Xueyimu , Tian Rong-Ming , Cai Jing , Tan Wei-Yan , Liu Chang-Kun , He Xing-Jin , Zhou Song-Dong 
Published: January 2025

BackgroundThe genus Cyclorhiza is endemic to China and belongs to the Apiaceae family, which is widely distributed in the Himalaya–Hengduan Mountains (HHM) region. However, its morphology, phylogeny, phylogeography, taxonomy, and evolutionary history were not investigated due to insufficient sampling and lack of population sampling and plastome data. Additionally, we found that Seseli purpureovaginatum was not similar to Seseli members but resembled Cyclorhiza species in morphology, indicating that the taxonomic position of S. purpureovaginatum needs to be re-evaluated.MethodsFirst, we observed the morphology of the genus. Second, we newly sequenced four plastomes and conducted comparative analyses. Third, we used the newly sequenced internal transcribed spacer (ITS) and chloroplast DNA (cpDNA) (matK, trnQ-rps16, and trnD-trnT) from 27 populations totaling 244 individuals to explore the genetic diversity and structure. Finally, we performed the phylogenetic analyses based on three datasets (plastome data, ITS sequences, and haplotypes) and estimated the origin and divergence time of the genus.Results and discussionThe morphology of Cyclorhiza plants and S. purpureovaginatum was highly similar, and their plastomes in structure and features were conserved. The genus possessed high genetic diversity and significant lineage geographic structure, which may be associated with the long-term evolutionary history, complex terrain and habitat, and its sexual reproduction mode. The genus Cyclorhiza originated in the late Eocene (36.03 Ma), which was closely related to the early uplift of the Qinghai–Tibetan Plateau (QTP) and Hengduan Mountains (HDM). The diversification of the genus occurred in the late Oligocene (25.43 Ma), which was largely influenced by the colonization of the newly available climate and terrain. The phylogenetic results showed that Cyclorhiza species clustered into a separate clade and S. purpureovaginatum nested within Cyclorhiza. Cyclorhiza waltonii was sister to Cyclorhiza peucedanifolia, and Cyclorhiza puana clustered with S. purpureovaginatum. Thus, based on the morphology, plastome analyses, and phylogenetic evidence, S. purpureovaginatum should be transferred to Cyclorhiza. All these evidences further supported the monophyly of the genus after including S. purpureovaginatum. Finally, we clarified the generic limits of Cyclorhiza and provided a species classification key index for the genus. In conclusion, the study comprehensively investigated the morphology, phylogeography, phylogeny, taxonomy, and evolution of the genus Cyclorhiza for the first time.
How can graduate students’ research pressure be transformed into motivation and innovative behavior? The role of graduate students’ mentorship homegate (or team) support
Authors: Li Qiming , Du Xian , Chen Hua , Zhou Xianli 
Published: January 2025

IntroductionThis study explores how graduate students’ mentorship homegate (or team) support (GSMTS) and challenging-hindering pressures impact their intrinsic motivation for research, identification with research roles, and innovative behaviors.MethodsData from 548 graduate students were collected using convenience sampling and analyzed using Amos and SPSS statistical software package via questionnaires distributed to universities in SiChuan province of China.ResultThe findings reveal that (1) research stress can not directly and positively predict innovative behaviors among graduate students, while intrinsic research motivation and research role identification mediate the relationship between research stress and graduate students’ innovative behavior; (2) hindering research pressure negatively impacts the intrinsic motivation for research, whereas challenging research pressure has a positive effect; (3) GSMTS directly fosters innovative behaviors among graduate students, with intrinsic motivation and roles’ identification for research as sequential mediators; and (4) GSMTS positively moderates the relationship between challenging research pressure and both the intrinsic motivation for research and role identity.DiscussionThis suggests that higher education institutions should cultivate an optimal research and innovation environment for graduate students by increasing challenging research pressure and reducing hindering pressure. They should also emphasize the development of graduate students’ intrinsic motivation for research and identification with research roles. Concurrently, the role of GSMTS should be highlighted to facilitate both the direct and indirect development of graduate students’ innovative behaviors.
Video-feedback intervention for promoting social engagement in children with neurodevelopmental disabilities
Authors: Montirosso Rosario , Micheletti Serena , Pisoni Camilla , Mascheroni Eleonora , Scarano Elisa , Naboni Cecilia , Rosa Elisa , Castagna Annalisa , Bonino Margherita , Fazzi Elisa , Orcesi Simona 
Published: January 2025

IntroductionChildren with neurodevelopmental disabilities (NDs) display several developmental impairments across various domains that impact parent–child interactions, emphasizing the need for effective early interventions. This multi-centric study aimed to evaluate the impact of video-feedback intervention (VFI) on enhancing maternal behavior (i.e., sensitivity) and socio-emotional skills (i.e., engagement and emotionality) in children with NDs during normal or stressful interactions (i.e., the Face-to-Face Still-Face, [FFSF]) paradigm.MethodsA single-cohort design with pre-(T0) and post-(T1) intervention assessment was used to evaluate 37 mother–child dyads from three units in Northern Italy. The children, aged between 6 and 24 months, had a diagnosis of ND, including psychomotor delay (n = 26) and cerebral palsy (n = 5). The VFI was administered over 6 weeks, with each session focusing on improving parents’ developmental supporting behaviors and enhancing the quality of parent–child interactions. Using the Global Rating Scale coding system (GRS), child behaviors (engagement and emotionality) and maternal behavior (sensitivity) were assessed during the FFSF.ResultsAnalyses of variance (ANOVA) indicated significant improvement post-intervention in child engagement in the Reunion episode, with an increase in mother-directed gaze communicative gestures and positive vocalization. A paired sample t-test revealed that the mother’s sensitivity significantly improves between T0 and T1 during the Reunion phase. Moreover, a higher increase in sensitive maternal behavior during the Reunion phase was associated with greater child engagement at T1 during the Reunion episode. No significant changes post-intervention were observed in the emotionality of the child.ConclusionThe VFI effectively enhanced socio-emotional skills and maternal sensitivity during parent–child interactions, particularly in recovering from interactive disruptions.
Relationship between Internet use and offline leisure activities among Chinese older adult people: a moderated mediation model
Authors: Xiang Shuai , Deng Qinwen , Chen Boli 
Published: January 2025

BackgroundThe process of aging in Chinese society is accompanied by the concurrent development of the Internet. In recent years, the influence of Internet use on the activities of older adults has attracted growing interest and is now a significant focus of both public health and gerontological research. Nevertheless, there is a need for further empirical research in this area.PurposeThis study aimed to examine the mechanisms and interaction processes, as well as the relationship between Internet use and offline leisure activities among Chinese older adults. Theories of active aging and environmental gerontology guided the construction and assessment of the mediating role of self-perception of aging between Internet use and offline leisure activities. Additionally, the moderating role of community-based leisure places (CBLP) was examined.MethodsA moderated mediation model was constructed using the SPSS 27 PROCESS Macro. This study employs the most recent China Longitudinal Aging Social Survey (CLASS) 2020 cross-sectional data, and the valid sample comprises 8,180 Chinese older adult individuals aged 60 years or older.Results(1) Internet use and offline leisure activities among older adults exhibited a significant positive correlation. (2) Self-perception of aging played a significant positive mediating role between Internet use and offline leisure activities. (3) CBLP significantly and positively moderated the relationship between Internet use and offline leisure activities.ConclusionThe Internet can be an effective tool for addressing the challenges of aging. Active Internet use may lead to more positive age perception and more active offline leisure activities, and efforts should be made to increase the digitization of the aging society. Positive self-perception of aging is an important psychological factor influencing the offline and online activities of older adults. It is imperative that the concept of active aging be vigorously promoted. Concurrently, community infrastructure serves as the foundation for the mutual promotion of online and offline activities. Attention should be given to the development of infrastructure for an age-friendly environment. These findings deepen the understanding of the consequences of interactions between online and offline activities, subjective mindsets, and objective environments for older adults.
High prevalence and co-occurrence of modifiable risk factors for non-communicable diseases among university students: a cross-sectional study
Authors: Cicekli Ipek , Gokce Eskin Serap 
Published: January 2025

BackgroundNon-communicable diseases (NCDs) are a major global concern. This study aimed to examine the prevalence and co-occurrence of lifestyle risk factors among university students.MethodsThis analytical, cross-sectional study was conducted between January and April 2022. A total of 485 students were included in the study. Lifestyle risk variables for NCDs included physical inactivity, low fruit and vegetable consumption, alcohol use, tobacco smoking, being overweight/obese, and sugar sweetened beverages consumption.ResultsOur results showed that the most frequent risk factor was insufficient physical activity (89.2%), followed by low vegetable (70.5%) and fruit consumption (58.9%). Overall, more than half of the students (51.2%) had four or more risk factors highlighting the urgent need for preventive interventions. The co-occurrence of four or more lifestyle risk factors was significantly greater in students at private universities (aOR: 2.01 95% CI: 1.2; 3.35), those living in student homes (aOR: 3.57 95% CI: 1.96; 6.5), and those with fast food preferences when eating outside (a0R: 2.53 95% CI: 1.62; 3.96).ConclusionTargeted university-based interventions, such as promoting physical activity, providing affordable nutritious meals, and educating students on healthy lifestyles, are essential to reduce non-communicable disease (NCD) risk among students. Early action fosters lifelong healthy habits, supports healthy aging, and reduces healthcare costs. Future research should focus on refining these strategies to maximize their impact on university populations.
Impact of peer interaction on maternal health service utilization in rural northwest China
Authors: Xiang Liuchun , Li Dan , Wu Junhao , Chen Jun , Yang Jie , Nie Haisong 
Published: January 2025

BackgroundEffective use of health services by pregnant and postpartum woman (PPWs) is crucial to maternal and child health. Most maternal deaths are attributed to inadequate maternal health services, especially in rural areas. As a vulnerable group, rural PPWs can effectively prevent and reduce maternal and infant health risk factors through whole-process health management and ensure the health and safety of mothers and infants. Therefore, improving the utilization rate of rural maternal health services is a key issue that needs to be addressed urgently. This study aimed to explore the influence of peer interaction on the utilization of maternal health services in rural areas and the mediating effect of maternal health service knowledge.MethodsBased on cross-sectional data of 821 PPWs in rural northwest China. This study used propensity score matching (PSM) to analyze the effects of peer interaction (PI) on maternal health service utilization, including maternal system management rate (Y1), prenatal screening rate (Y2), and postpartum visit rate (Y3). In addition, the mediating role of maternal health service knowledge between peer interaction and health service utilization was empirically tested.ResultsThe findings highlight the important role of peer interaction in improving the utilization rate of maternal health services in rural northwest China. The study found that peer interaction significantly improved the maternal system management rate, prenatal screening rate, and postpartum visit rate. At the same time, peer interaction enhances knowledge of maternal health services, which plays a key role in improving maternal health behaviors.ConclusionThrough experience sharing and knowledge exchange among peers, understanding of health services can be enhanced, and positive health behaviors can be promoted. Policymakers and healthcare providers should integrate peer support programs into existing maternal health initiatives and fully use social media and community resources to create interactive platforms for maternal and infant knowledge that combine online and offline. By actively promoting peer interaction and experience sharing, these initiatives can maximize the positive role of peer support, increase the utilization rate of health services, and effectively ensure their safety.