bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection
Authors: Shang Kaijie , Xiao Li , Zhang Xianping , Zang Lianyi , Zhao Dan , Wang Chenchen , Wang Xipan , Zhou Tao , Zhu Changxiang , Zhu Xiaoping 
Published: February 2023

AbstractTomato chlorosis virus (ToCV) is a member of the genus Crinivirus in the family Closteroviridae. It has a wide host range and wide distribution, causing serious harm to the vegetable industry. The autophagy pathway plays an important role in plant resistance to virus infection. Viruses and plant hosts coevolve in defence and antidefence processes around autophagy. In this study, the interaction between ToCV p22 and Nicotiana benthamiana B‐cell lymphoma2‐associated athanogenes5 Nicotiana benthamiana (NbBAG5) was examined. Through overexpression and down‐regulation of NbBAG5, results showed that NbBAG5 could negatively regulate ToCV infection. NbBAG5 was found to be localized in mitochondria and can change the original localization of ToCV p22, which is colocalized in mitochondria. NbBAG5 inhibited the expression of mitophagy‐related genes and the number of autophagosomes, thereby regulating viral infection by affecting mitophagy. In summary, this study demonstrated that ToCV p22 affects autophagy by interacting with NbBAG5, established the association between viral infection, BAG proteins family, and the autophagy pathway, and explained the molecular mechanism by which ToCV p22 interacts with NbBAG5 to inhibit autophagy to regulate viral infection.
Could Sufism Have Been a Means of Spreading Ibn Taymiyya's Thought in the Ottoman Empire?
Authors: Dumairieh Naser 
Published: February 2023

AbstractCurrent studies on Ibn Taymiyya's influence on the intellectual life of the Ottoman Empire focus on the mid‐sixteenth and the early seventeenth centuries. In this paper, I argue that Ibn Taymiyya's influence on some aspects of Ottoman intellectual life can be traced, indirectly, to the beginning of the fifteenth century. Through studying the attitudes of some scholars toward Ibn ʿArabī and his ideas, I argue that these attitudes were affected by the outflow of Ibn Taymiyya's ideas. How did these ideas leak into the Ottoman intellectual milieu? Unexpectedly, it seems that Sufism played an important role in spreading some of Ibn Taymiyya's ideas in an indirect way. This paper discusses three possible ways through which Ibn Taymiyya's thought may have circulated in the Ottoman Empire as early as the fifteenth century: a scholar, a book, and a Sufi order.
Majlis Taklim and The Path Of Women's Islamization In Indonesia
Authors: Latief Hilman , Madjid Abd. 
Published: February 2023

AbstractWomen's religious gatherings or Islamic study circles in Indonesian public spaces have become remarkably diverse. These informal learning groups, referred to as majlis taklim, have flourished across Indonesia, from large towns to small villages, and have become places for Muslim communities to increase their awareness of Islam and improve their spirituality. Although majlis taklim are non‐gendered organizations, they are formed in many places by women's groups with different social and economic backgrounds. This paper analyzes the adaptability of women‐run majlis taklim in terms of offering and religious discourse to transform society's spiritual and social lives. Apart from nurturing members' self‐spiritual feelings, the models of majlis taklim in formulating discourse and addressing women participants represent Islamization's trajectory in Indonesia, characterized by two types of religious orientations: domesticating discourse and empowerment efforts.
Re‐Visiting the Meaning of ‘ẓann’ in the Qurʾān
Authors: Galadari Abdulla 
Published: February 2023

AbstractThe Qurʾānic term, ‘ẓann,’ is usually understood and translated as conjecture. However, I argue that the Qurʾān uses ‘ẓann’ to mean dogmatic zeal or, in other words, being zealous to a certain belief. For conjecture, the Qurʾān uses the root ‘ḥ‐s‐b,’ such as, ‘ayaḥsabu.’ Although the Qurʾān may criticize some people's conjectures, it does not criticize the act of formulating opinions with the root ‘ḥ‐s‐b.’ However, the Qurʾān does criticize the act of ‘ẓann.’ This further emphasizes the distinction between conjecture and ‘ẓann,’ according to the Qurʾān. The main emphasis is that when the Qurʾān requires people to shun most ‘ẓann,’ it is argued that it is asking to shun zealous beliefs and dogmas, and it is not asking to shun the formulation of conjectures. The method used is philological, in which the cognates are analyzed in their contexts and compared with their uses in the Qurʾān. Defining ‘ẓann’ as dogmatic zeal rather than conjecture has far‐reaching implications in understanding Qurʾānic epistemology and the epistemic process it expects its audience to have.
Kaempferol‐3‐rhamnoside overaccumulation in flavonoid 3′‐hydroxylase tt7 mutants compromises seed coat outer integument differentiation and seed longevity
Authors: Niñoles Regina , Arjona Paloma , Azad Sepideh M. , Hashim Aseel , Casañ Jose , Bueso Eduardo , Serrano Ramón , Espinosa Ana , Molina Isabel , Gadea Jose 
Published: February 2023

Summary

Seeds slowly accumulate damage during storage, which ultimately results in germination failure. The seed coat protects the embryo from the external environment, and its composition is critical for seed longevity. Flavonols accumulate in the outer integument. The link between flavonol composition and outer integument development has not been explored.

Genetic, molecular and ultrastructural assays on loss‐of‐function mutants of the flavonoid biosynthesis pathway were used to study the effect of altered flavonoid composition on seed coat development and seed longevity.

Controlled deterioration assays indicate that loss of function of the flavonoid 3′ hydroxylase gene TT7 dramatically affects seed longevity and seed coat development. Outer integument differentiation is compromised from 9 d after pollination in tt7 developing seeds, resulting in a defective suberin layer and incomplete degradation of seed coat starch. These distinctive phenotypes are not shared by other mutants showing abnormal flavonoid composition. Genetic analysis indicates that overaccumulation of kaempferol‐3‐rhamnoside is mainly responsible for the observed phenotypes. Expression profiling suggests that multiple cellular processes are altered in the tt7 mutant.

Overaccumulation of kaempferol‐3‐rhamnoside in the seed coat compromises normal seed coat development. This observation positions TRANSPARENT TESTA 7 and the UGT78D1 glycosyltransferase, catalysing flavonol 3‐O‐rhamnosylation, as essential players in the modulation of seed longevity.
Persulfidation protects from oxidative stress under nonphotorespiratory conditions in Arabidopsis
Authors: García‐Calderón Margarita , Vignane Thibaut , Filipovic Milos R. , Ruiz M. Teresa , Romero Luis C. , Márquez Antonio J. , Gotor Cecilia , Aroca Angeles 
Published: February 2023

Summary

Hydrogen sulfide is a signaling molecule in plants that regulates essential biological processes through protein persulfidation. However, little is known about sulfide‐mediated regulation in relation to photorespiration.

Here, we performed label‐free quantitative proteomic analysis and observed a high impact on protein persulfidation levels when plants grown under nonphotorespiratory conditions were transferred to air, with 98.7% of the identified proteins being more persulfidated under suppressed photorespiration. Interestingly, a higher level of reactive oxygen species (ROS) was detected under nonphotorespiratory conditions.

Analysis of the effect of sulfide on aspects associated with non‐ or photorespiratory growth conditions has demonstrated that it protects plants grown under suppressed photorespiration. Thus, sulfide amends the imbalance of carbon/nitrogen and restores ATP levels to concentrations like those of air‐grown plants; balances the high level of ROS in plants under nonphotorespiratory conditions to reach a cellular redox state similar to that in air‐grown plants; and regulates stomatal closure, to decrease the high guard cell ROS levels and induce stomatal aperture. In this way, sulfide signals the CO2‐dependent stomata movement, in the opposite direction of the established abscisic acid‐dependent movement.

Our findings suggest that the high persulfidation level under suppressed photorespiration reveals an essential role of sulfide signaling under these conditions.
Seed priming can enhance and retain stress tolerance in ensuing generations by inducing epigenetic changes and trans‐generational memory
Authors: Louis Noble , Dhankher Om Parkash , Puthur Jos T. 
Published: February 2023

AbstractThe significance of priming in enhancing abiotic stress tolerance is well‐established in several important crops. Priming positively impacts plant growth and improves stress tolerance at multiple developmental stages, and seed priming is one of the most used methods. Seed priming influences the pre‐germinative metabolism that ensures proper germination, early seedling establishment, enhanced stress tolerance and yield, even under unfavourable environmental conditions. Seed priming involves pre‐exposure of seeds to mild stress, and this pre‐treatment induces specific changes at the physiological and molecular levels. Interestingly, priming can improve the efficiency of the DNA repair mechanism, along with activation of specific signalling proteins and transcription factors for rapid and efficient stress tolerance. Notably, such acquired stress tolerance may be retained for longer duration, namely, later developmental stages or even subsequent generations. Epigenetic and chromatin‐based mechanisms such as DNA methylation, histone modifications, and nucleosome positioning are some of the key molecular changes involved in priming/stress memory. Further, the retention of induced epigenetic changes may influence the priming‐induced trans‐generational stress memory. This review discusses known and plausible seed priming‐induced molecular mechanisms that govern germination and stress memory within and across generations, highlighting their role in regulating the plant response to abiotic stresses. Understanding the molecular mechanism for activation of stress‐responsive genes and the epigenetic changes resulting from seed priming will help to improve the resiliency of the crops for enhanced productivity under extreme environments.
Water permeability of different aerial tissues of carnations is related to cuticular wax composition
Authors: Cheng Guiping , Ye Changchun , Zhang Jiajun , Li Hongmei , Jiang Yueming , Duan Xuewu 
Published: February 2023

AbstractCuticular wax protects aerial plant tissues against uncontrolled water loss. To compare the differences among tissues, cultivars, and postharvest stages, we characterized the surface morphology, water permeability, and chemical composition of cuticular wax on the leaf, calyx, and petals of two carnation cultivars (‘Master’ and ‘Lady green’) at two postharvest stages. Obvious differences in these characteristics were found among tissues but not among cultivars or postharvest stages. The leaf surface was relatively smooth, whereas convex cells were observed on the petals. The mean minimum conductance of leaves was significantly higher than that of the calyx, followed by that of petals. It ranged between 8.8 × 10−4 m s−1 for ‘Lady green’ leaves at Stage II and 3.6 × 10−5 m s−1 for ‘Master’ petals at Stage I. Petal wax contained high concentrations of n‐alkanes, whereas primary alcohols dominated in leaf wax. The weighted average chain length (ACL) was higher in petal wax than in leaf wax; it ranged from 19.6 in ‘Lady green’ leaves to 24.14 in ‘Lady green’ petals at Stage I. In conclusion, carnation petals are characterized by numerous convex cells on both the adaxial and abaxial surfaces, and their main cuticular wax components, alkanes, have a higher ACL than leaf cuticular wax, which contributes to their higher water barrier property. The results provide further evidence for the association between cuticular chemical composition and the physiological function of the cuticle in blocking water transpiration.
The moderating role of attachment in the association between physiological synchrony in married couples and supportive behavior in the transition to parenthood
Authors: Fogel‐Yaakobi Shlomit , Golland Yulia , Levit‐Binnun Nava , Borelli Jessica L. , Mikulincer Mario , Shai Dana 
Published: February 2023

AbstractDespite supportive behaviors playing a central role in intimate relationships, the extent to which physiological and psychological factors are involved in the quality of the observed spousal support, remains largely unknown. From a physiological stance, cardiac synchrony has been identified as an important component involved in dyadic interpersonal interactions. This study aims to examine whether individual differences in attachment determine, at least to some extent, whether cardiac synchrony enhances or impedes the quality of the observed spousal support. Specifically, this study examines whether attachment style moderates the biobehavioral link between positive cardiac synchrony and observed spousal support. A total of 58 couples expecting their first child participated in a supportive interaction while their cardiovascular responses were recorded, and the quality of their caregiving behaviors was coded. Results indicated that couples' cardiovascular dynamics were synchronized during the supportive interaction. Furthermore, attachment moderated the association between positive cardiac synchrony and the behavioral manifestation of support, such that cardiac synchrony was negatively associated with the quality of support when offered by caregivers with higher scores of attachment anxiety. Our findings show that for those caregivers who have higher levels of anxious attachment—being synchronized with their care seeking spouses is associated with difficulties in providing effective support. We discuss these findings in the context of the emotion regulation difficulties characteristic of anxious attachment patterns. These findings suggest that future work could benefit from performing comprehensive studies that consider physiological, behavioral, and psychological constructs simultaneously.
Does frequency or duration of standing breaks drive changes in glycemic response? A randomized crossover trial
Authors: Toledo Meynard John L. , Ainsworth Barbara E. , Gaesser Glenn A. , Hooker Steven P. , Pereira Mark A. , Buman Matthew P. 
Published: February 2023

Intervention strategies to break up sitting have mostly focused on the modality (i.e., comparing different intensities and/or type of activities) and less on how frequency and duration of breaks affect health outcomes. This study compared the efficacy of different strategies to break up sitting time [i.e., high frequency, low duration standing breaks (HFLD) and low frequency, high duration standing breaks (LFHD)] in reducing postprandial glucose. Eleven sedentary and prediabetic adults (mean ± SD age = 46.8 ± 10.6 years; 73% female) participated in a cross‐over trial. There were six blocks that represented all potential combinations (ordering) of the study conditions and participants were randomly assigned to a block. Each participant underwent three 7.5‐h laboratory visits (1 week apart) where they engaged in either continuous sitting, HFLD, or LFHD condition while performing their usual office‐related tasks. Standardized breakfast and lunch meals were provided. Postprandial mean glucose, area under the curve (AUC), and incremental area under the curve (iAUC) were evaluated using mixed models. Compared with LFHD condition, the HFLD standing breaks condition significantly lowered mean glucose by −9.94 (−14.13, −5.74) mg/dL·h after lunch, and by −6.23 (−9.93, −2.52) mg/dL·h, for the total lab visit time. Overall, the results favor frequently interrupting sitting with standing breaks to improve glycemic control in individuals with prediabetes. Further studies are needed with larger sample sizes to confirm the results.
Sustainable development and sacrifice in the rural North
Authors: Stiernström Arvid 
Published: February 2023

AbstractIn this article, I examine how the grand narratives of sustainability, development and mining impact local governance in a Swedish municipality. I do this by studying three mining projects under implementation and relate them to notions of development and sacrifice to lend insights into what the new trends of mining in Europe outlined above mean for the rural North. I regard sustainable development as a political concept ascribed to activities (such as political programmes or investments). Based on ethnographic fieldwork in the Storuman municipality, I show how politicians, political parties and government administrators position themselves in relation to the three mining projects. I provide a detailed account of perceptions, responses and practices in a local government that engage with three mining companies seeking to open mines within the municipal borders. I show how a tug of war between ideas of sustainable development or survival through sacrifice depoliticises and stabilises a dominant political line in favour of mining within local politics. While the respondents differed in what they thought that sacrifice might lead to, ranging from environmental disaster and social suffering to economic prosperity, I reveal a process wherein sustainable development becomes translated into ‘that which must be sacrificed’.
How do I save cell therapy product's storage space?
Authors: Kim Grace H. , Patel Nita , Reich‐Slotky Ronit 
Published: February 2023

AbstractBackgroundAvailability of liquid nitrogen (LN2) freezer storage space is a major challenge for many transplant programs as they continue to grow and accumulate products. The recent trend of allogeneic grafts cryopreservation that started during the COVID‐19 pandemic, made the situation even worse requiring an increase in storage capacity. Multi‐compartment cryopreservation bags can help save storage space but can be tricky to use. Here, we describe the validation of muti‐compartment cryopreservation bags for the purpose of donor lymphocyte infusion (DLI) aliquots.MethodsWe validated the use of five compartment cryobags for cryopreservation of cell therapy products. Four products were cryopreserved using these bags and each compartment was tested post‐thaw for product volume distribution, total cell count recovery, and viability. Additionally, the integrity of both bag compartments and labels was assessed as well.ResultsAll tested specimens met post‐thaw viability and TNC recovery acceptability criteria. Fill volume was optimized at 24–25 mL for acceptable volume distribution between aliquots. With proper heat sealing between compartments, all aliquots retain their integrity and cryopreservation labels were adherent and legible.ConclusionsMuti‐compartment bags can be used successfully for cryopreservation of cell therapy products and increase storage capacity.
Effect of Bee Pollen Kelulut Bees on HBA1C in Type 2 Diabetes Mellitus Patients
Authors: Setyawan Annaas Budi , Satria Andri Praja , Arung Enos Tangke , Paramita Swandari 
Published: February 2023

Bee pollen has been used for generations to treat various diseases, including diabetes mellitus, because it inhibits alpha glucosidase enzyme. Two major problems suffered by patients with type 2 Diabetes Mellitus (DM) are linked to insulin resistance and impaired insulin secretion. They often occur in patients aged above 30 years and obese patients. High blood glucose and other complications, including increased HBA1C, are some of the risks faced by people with type 2 diabetes mellitus. This study aimed to reveal the efficacy of bee pollen in lowering the HBA1C levels of patients with type 2 DM. Subjects were gave bee pollen kelulut bees to the intervention group, twice a day in the morning during 4 weeks. This study was quasy experimental research. It involved 30 patients who were divided into the intervention group and the control group. Data were analysis determined the differences in the control and intervention groups using the Mann Whitney test. The result of pre- and post-group was p value=0.001(<0.05), meaning that there was a significant difference in median cholesterol levels, while the result difference of the two groups was p value=0.033 (<0.05), implying there was a significant difference in HBA1C levels of the two groups. Bee pollen has been shown to lower HBA1C and have a potential as a non-pharmacological treatment in patients with type 2 DM.
Severity of coeliac disease and clinical management study when using a non-metabolised medication: a phase I pharmacokinetic study
Authors: Chretien Marc L , Bailey David G , Asher Linda , Parfitt Jeremy , Driman David , Gregor Jamie , Dresser George K 
Published: February 2023

ObjectiveThe non-metabolised antihistamine fexofenadine has oral absorption resulting from transporter activity. Uptake by enterocyte organic anion transporting polypeptides and efflux by an ATP-binding cassette transporter (P-glycoprotein) are primary determinants. Coeliac disease-mediated lesions to the small intestinal mucosa may alter oral absorption of the drug probe, fexofenadine.DesignA phase I, open-label, single-dose, pharmacokinetic studySettingLondon, Ontario, CanadaParticipantsPatients with coeliac disease (n=41) with positive serology and healthy individuals (n=48).Main outcome measuresPatients with coeliac disease—duodenal histology and oral fexofenadine pharmacokinetics within a 3-week period. Healthy individuals—oral fexofenadine pharmacokinetics with water and grapefruit juice.ResultsPatients with coeliac disease were stratified by disease severity: Group A (n=15, normal), B+C (n=14, intraepithelial lymphocytosis with/without mild villous blunting) and D (n=12, moderate to severe villous blunting). Patients with coeliac disease in groups A, B+C and D and healthy individuals receiving water had similar fexofenadine AUC0–8(2038±304, 2259±367, 2128±410, 1954±138 ng.h/mL; p>0.05; mean±SEM) and Cmax (440±73, 513±96, 523±104, 453±32 ng/mL; p>0.05), respectively. These four groups all had higher fexofenadine AUC0–8(1063±59; p<0.01) and Cmax (253±18; p<0.05) compared with those for healthy individuals receiving grapefruit juice. Coeliac groups had a positive linear trend between disease severity and fexofenadine Tmax (2.0±0.3, 2.7±0.4, 3.1±0.5 hours; p<0.05).ConclusionsCoeliac disease severity based on duodenal histopathology did not affect oral fexofenadine bioavailability. Increased Tmax suggested absorption distal to the duodenum (jejunum + ileum), where histology seems more normal which may be the key determinant. Patients with coeliac disease may not require consideration for alternative clinical drug management for a number of non-metabolised and transport-mediated medications.