bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silver nitrate (AgNO3) and Pyrazinamide (PZA) as ethylene inhibitors ameliorate in vitro salt tolerance of tomato (Lycopersicon esculentum L) plant.
Authors: Zarei Somayeh , Ehsanpour Ali Akbar 
Published: February 2023

Abstract
The purpose of this research was to find out how in vitro culture of tomato explants respond to salt stress by silver nitrate (AgNO3) and pyrazinamide (PZA) as ethylene inhibitors. Tomato seedlings were grown on MS medium containing NaCl (0, 100, 150 mM) and supplemented with AgNO3 (0, 2, 4, mg. L-1) and pyrazinamide (PZA) (0, 2, 4,mg. L-1) to study growth parameters and antioxidant enzymes responses. Salt-stressed plants showed limited growth and a significant decrease in fresh and dry weight. Salinity accelerated oxidative damage by increasing hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels in tomato leaves. In the present of AgNO3 and PZA, in vitro grown tomato plants increased fresh and dry weight total chlorophyll, carotenoid in particular at 4 mg. L-1 AgNO3 and 4 mg. L-1 PZA. Moreover, silver nitrate and PZA reduced H2O2 and MDA contents and oxidative damage by enhancing antioxidant enzyme activity, including catalase, superoxide dismutase, and ascorbate peroxidase under salt stress. According to our findings, AgNO3 and PZA improved in vitro salinity tolerance of tomato plant by interfering ethylene action or ethylene generation and increasing biochemical responses.
Stochastic-based vulnerability curves for the out-of-plane seismic safety assessment of URM walls
Authors: Bernardo Vasco , Costa Alfredo Campos , Lourenço Paulo B. 
Published: February 2023

Abstract
Earthquakes are a major cause of damage and human losses to the built environment, including cultural heritages, monumental buildings and historical centers. In the last decades the seismic performance of buildings has received special attention due to the interest in the built heritage conservation and protection of human life, particularly with respect to masonry structures which have shown evidence of poor behavior once subjected to seismic loads. The present work contributes to the seismic safety assessment of the out-of-plane behavior of unreinforced masonry walls through a displacement-based approach, providing the capacity for different out-of-plane geometric indexes and its seismic response in different earthquake-prone regions. The analyses are conducted using a seismic probabilistic framework, considering the most common out-of-plane mechanisms, different material properties, various slenderness ratios, and a wide range of seismicity levels to cover the seismic hazard in Europe. The results presented can be useful for seismic safety assessment and to incorporate vulnerability models for seismic risk analysis.
KK-LC-1, a biomarker for prognosis of immunotherapy for primary liver cancer
Authors: Zhu Sihui , Jin Yuncheng , Zhou Mingzhen , Li Lin , Song Xueru , Su Xinyu , Liu Baorui , Shen Jie 
Published: February 2023

Abstract
Purpose
There is mounting evidence that patients with liver cancer can benefit from Immune checkpoint inhibitors. However, due to the high cost and low efficacy, we aimed to explore new biomarkers for predicting the efficacy of immunotherapy.
Methods
Specimens and medical records of liver cancer patients treated at Drum Tower Hospital of Nanjing University were collected, and the expression of Kita-Kyushu lung cancer antigen-1 (KK-LC-1) in tissues as well as the corresponding antibodies in serum were examined to find biomarkers related to the prognosis of immunotherapy and to explore its mechanism in the development of liver cancer.
Results
KK-LC-1 expression was found to be 34.4% in histopathological specimens from 131 patients and was significantly correlated with Foxp3 expression (P = 0.0356). The expression of Foxp3 in the tissues of 24 patients who received immunotherapy was significantly correlated with overall survival (OS) (P = 0.0247), and there was also a tendency for prolonged OS in patients with high expression of KK-LC-1. In addition, the expression of KK-LC-1 antibody in the serum of patients who received immunotherapy with a first efficacy evaluation of stable disease (SD) was significantly higher than those with PR (P = 0.0413).
Conclusions
Expression of KK-LC-1 in both tissues and serum has been shown to correlate with the prognosis of patients treated with immunotherapy, and KK-LC-1 is a potential therapeutic target for oncological immunotherapy.
Comprehensive Analysis of KNSTRN Immunoassays and KNSTRN-related ceRNA Network in Hepatocellular Carcinoma
Authors: Jin Liang , Zhang Xiaojing , Fan Ming , Li Weimin , Lai Quanyou , Peng Wei , Zhang Xuan 
Published: February 2023

Abstract
Background: The role of Kinetochore-localized astrin/SPAG5-binding protein (KNSTRN) in Hepatocellular Carcinoma (HCC) remains not fully determined.
Methods: Based on Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases, we first investigated the potential oncogenic functions of KNSTRN Using R and various computational tools in HCC.
Results: Detailed results revealed that elevated expression of KNSTRN was considerably associated with bad overall survival (HR=1.48, 95%CI 1.05-2.09, P= 0.027) and progress free interval (HR=1.41, 95%CI 1.05-1.89, P= 0.021) in HCC. GO/KEGG functional enrichment analysis showed that KNSTRN is closely related to chromosome segregation, spindle, ATPase activity, tubulin binding, and cell cycle signaling pathway. TIMER databases analysis showed the correlationsbetween KNSTRN expression and tumor-infiltrating immune cells, biomarkers of immune cells and immune checkpoint expression. Moreover, KNSTRN level was significantly positively associated with immunosuppressive cell in tumor microenvironment (TME), including regulatory T cells (Tregs), myeloid derived suppressor cells (MDSCs), and cancer-associated fibrocytes (CAFs). Finally, the two possible ceRNA networks in LIHC were constructed by correlation analysis, and all these ceRNA networks contained KNSTRN.
Conclusion: KNSTRN can be used as a novel biomarker for the diagnosis and prognosis of HCC, and is related to tumor immune infiltration and ceRNA network.
Bufotalin-loaded biomimetic prussian blue nanoparticles for colorectal cancer chemo-photothermal therapy via induce ferroptosis
Authors: Wu Yi , Tian Jiahui , Luo Mengjie , Wu Zhengchun , Cao Ruiyun , Fu Chunyan , Yang Jialu , Peng Qian , Liu Rushi , Liu Bin 
Published: February 2023

Abstract
Background: As a ferroptosis-inducing platform, based on its release of iron and ferrous ions to induce production of reactive oxygen species (ROS), it has attracted extensive attention in cancer treatment. Since iron-based nanomaterials such as prussian blue (PB), can only produce limited ROS. Bufotalin (CS-5), a promising ferroptosis inducer, was introduced to improve the efficiency of ferroptosis. However, the clinical application of CS-5 was limited by cardiotoxicity and high renal clearance rate. Therefore, we constructed a biomimetic nanosystem (PB@CS-5@M) with satisfactory tumor enrichment ability and long blood circulation time for the treatment of colorectal cancer induced by ferroptosis.
Results: In this work, the PB@CS-5@M has efficient loading of CS-5, which significantly reduces the cardiotoxicity of CS-5. After photothermal therapy, the high temperature caused by laser stimulation triggers the release of CS-5, iron and ferrous ions to kill colorectal cancer cells. Furthermore, the ability of CS-5 to significantly down-regulate the expression of GPX4 and HIF-1a, induce ROS production and damage mitochondria to induce ferroptosis in colorectal cancer was amplified. In addition, the down-regulation of HSP70 expression under laser stimulation effectively improved the chemosensitivity of HCT116 cells to CS-5.
Conclusions: The chemotherapeutic effect of CS-5 combined with the photothermal effect of PB can effectively treat colon cancer by inducing ferroptosis.
Paradoxical behaviour of rainfall and temperature over ecologically sensitive areas along the Western Ghats
Authors: Mann Rohit , Saini Deepak , Sharma Swati , Dhorde Amit , Gupta Anju 
Published: February 2023

Abstract

Initial reports signify some specific isolated locations in different latitudes, revealing a paradoxical increase in both heavy and very heavy rainfall events and also an increment in total, i.e., in both rainfall and temperature, over ecologically sensitive areas along the Western Ghats (WG). This paper presents a coherent study of the full-scale of daily rainfall and temperature over 27 well-spaced stations in the study area to determine its extent and investigate whether or not this contradictory behaviour is real. Also, an attempt has been made to assess the differential behaviour of rainfall, temperature, and heavy rainfall events in association with land use and land cover change (LULC). The analysis revealed that rainfall and temperature over the study area are increasing, whereas heavy rainfall events have increased during 1981–2020 with strong peaks after 2000 around 18o-19oN (Mumbai metropolitan region), 14o-16oN (mining and quarrying regions in Goa), and 9o-12oN (a narrow strip of land spanning across the coastal towns of Karnataka and Kerala) latitudes. The majority of the rainfall excess years coincided with El-Nino years, indicating that El-Nino does not affect rainfall negatively. However, rainfall over the WG is influenced by local relief and cascading topography. The spatial pattern of average annual rainfall shows a decreasing trend from south to north because the elevation and span of rainfall occurrence are higher in the southern part of WG. The findings of the current research will help in building a strategy to address trends and patterns of climatic variables in association with LULC.
Production and quality evaluation of corn crackers fortified with freeze dried of banana peel and pulp
Authors: Mahmoud Khaled , Shedeed Naglaa , Hussein Ahmed 
Published: February 2023

Abstract
Crackers made from corn flour fortified with FDB-peel or FDB-pulp or FDB-peel and FDB-pulp. Results showed the moisture and total carbohydrate, were highest in FDB-Pulp while the FDB-Peel presented the highest ash, protein and dietary fiber. Higher content vitamin C in FDB-peel which is rich in potassium, phosphorus and calcium. Ferulic acid and Sinapic dominated phenolic profile of the FDB pulp and peel. Crackers sample (F5) showed a significant increase in dietary fiber and low calories. The sensory evaluation of (F3) had high score of overall acceptability; while (F2) got the lowest score on color and appearance. Present of Choline and vitamin C were increased as a result of adding the FDB peel and pulp powder to crackers. The total phenolic and antioxidants were increased with addition of FDB peel and pulp powder in crackers sample. The control samples were the most clearly oxidized, which was evidenced by the lowest IP values obtained. The lipid IP values of (F2) samples were found to be significantly higher. The peroxide and acid value in crackers samples were low compere with control. The FDB-pulp and FDB-peel products contain great deal of nutritional properties, and hence have potential utility for use as functional ingredient in food product development.
“No matter what, we just work with the trauma…”: Mental Health Therapists’ Care of Diverse Sexual and Gender Identity Citizens in Cambodia
Authors: Bertram Julie E. , Kryah Rachel , Vandermause Roxanne , Ean Nil , Paul Robert , Carrico Adam , Sophal Chhit , Bruce Steven , Gregory Kelly , Stein Ellen , Mannarino Julie 
Published: February 2023

Abstract
The convergence of trauma symptomatology, mental health symptoms, family and social difficulties, and intersectionality of diverse sexual and gender minority (SGM) individual issues is complex, multi-faceted, and challenging for the individuals in Cambodia who suffer them and for the therapists in Cambodia who meet individuals in treatment. We documented and analyzed the perspectives of mental health therapists in the context of a randomized control trial (RCT) intervention within the Mekong Project in Cambodia. The research questions explored perceptions of therapists’ care of mental health clients, therapist well-being, and experiences of navigating within a research environment in which SGM citizens with mental health concerns receive treatment. The larger study enrolled 150 Cambodian adults, among which 69 identified as SGM. Three key patterns emerged across our interpretations. Clients seek help when symptoms
interfere with daily life, therapists care for clients and themselves, and integrated research and practice is integral yet sometimes paradoxical. Therapists did not identify differences in terms of how they work with SGM clients compared with non-SGM clients. Future studies are warranted to examine a reciprocal academic-research partnership in which we examine therapists’ work alongside rural community members, evaluate the process of embedding and fortifying peer supports within educational systems, and study the wisdom of traditional and Buddhist healers to address the discrimination and violence that citizens who identify as SGM disproportionately suffer. National Library of Medicine (U.S.). (2020). Trauma Informed Treatment Algorithms for Novel Outcomes (TITAN). Identifier NCT04304378.
Voice reacquisition after total laryngectomy via tracheoesophageal speech in patients with head and neck cancer: A qualitative study
Authors: Iwai Miyoko , Ando Shoko , Sato Kazuki 
Published: February 2023

Abstract
Purpose: Speech rehabilitation contributes to improved quality of life for patients who have undergone laryngectomy for head and neck cancer. Tracheoesophageal speech (TES) provides better speech quality when rehabilitation is initiated early. However, patients must work on rebuilding their self-identity and develop the habit of cleaning and replacing their voice prostheses regularly. We aimed to attain a deeper theoretical understanding of the process involved in the transition from selection to adaptation to TES in patients with head and neck cancer.
Methods: Using grounded theory methodology, we interviewed 13 TES speakers (3 women) across peer support groups in Japan who had undergone laryngectomy for head and neck cancer. Semi-structured interviews were used to investigate what TES speakers thought of their voices, their preferred means of alternative communication, and how they have adapted.
Results: Participants emphasized the importance of communicating with their own voices, the adaptation process for which was influenced by anxiety related to losing one’s natural voice, confronting the reality of living without a voice, and coping with one’s new voice. Through this process, participants eventually learned to recognize the changed voice as one's own. However, despite voice restoration through TES, feelings of hopelessness persisted for many patients.
Conclusion: The value assigned “communicating with one’s own voice” is integral to the adaptation process for patients with head and neck cancer who undergo laryngectomies and use TES. However, some aspects of TES can easily lead to feelings of hopelessness, suggesting the importance of enhancing multifaceted support for speech rehabilitation.
An Intelligent and Hybrid PDOA+MO based MPPT Controlling Mechanism for Grid-PV Systems
Authors: Marlin S , Jebaseelan Sundarsingh 
Published: February 2023

Abstract
Due to its effectiveness and affordability, solar Photovoltaic (PV) systems are now being employed more and more in many application systems. The biggest challenge is still getting the most energy out from PV panels in a variety of climatic situations. In order to achieve the maximum energy production, several optimization-based MPPT controlling techniques have been developed in traditional works. However, it has significant issues with low convergence, computational complexity, a long time needed to find the best solution, and inefficiency. As a result, the goal of this research is to implement a novel and hybrid optimization technique, named as, Prairie Dog Optimization Algorithm (PDOA) + Mongoose Optimization (MO) for extracting the highest possible energy from PV panels. The original contribution of this work is to incorporate the functions of two different and recently developed optimization techniques for MPPT controlling. For this purpose, the PDOA and MO algorithms are taken into account, and the hybridized PDOA+MO provides the benefits of fast convergence, increased tracking efficiency, and reduced tracking time. The high gain Luo DC-DC converter is also used to increase PV's output power and voltage while minimising switching and conduction losses. Consequently, the voltage source inverter is used to suppress the level of harmonics for providing the quality improved AC output to the grid system. The suggested PDOA+MO algorithm's effectiveness and power tracking performance are validated through a thorough simulation analysis using a variety of parameters.
Llamas use social information from conspecifics and humans to solve a spatial detour task
Authors: Pahl Annkatrin , von Borstel Uta König , Brucks Désirée 
Published: February 2023

Abstract
Learning by observing others (i.e. social learning) is an important mechanism to reduce the costs of individual learning. Social learning can occur between conspecifics but also heterospecifics. Domestication processes might have changed the animals’ sensitivity to human social cues and recent research indicates that domesticated species are particularly good in learning socially from humans. Llamas (Lama glama) are an interesting model species for that purpose. Llamas were bred as pack animals, which requires close contact and cooperative behaviour towards humans. We investigated whether llamas learn socially from trained conspecifics and humans in a spatial detour task. Subjects were required to detour metal hurdles arranged in a V-shape to reach a food reward. Llamas were more successful in solving the task after both a human and a conspecific demonstrated the task compared to a control condition with no demonstrator. Individual differences in behaviour (i.e. food motivation and distraction) further affected the success rate. Animals did not necessarily use the same route as the demonstrators. This indicates that they did not copy the behaviour of the demonstrator but rather adopted the general detour behaviour. These results support the hypothesis that domestication affected the sensitivity to human social cues but also highlights the need to expand the range of domesticated species that are tested in this kind of paradigms.
Mental stress detection using multimodal characterization of PPG signal for personal healthcare applications
Authors: Paul Avishek , Chakraborty Abhishek , Sadhukhan Deboleena , Pal Saurabh , Mitra Madhuchhanda 
Published: February 2023

Abstract
With growing complexities in our society, mental stress has become inevitable in every human life. Long-term mental stress condition could instigate several chronic diseases and thus require its early evaluation. Existing mental stress estimation techniques mostly uses complicated, multi-channel and expert dependent electroencephalogram (EEG) based approaches. Moreover, the respiratory signal presents promising stress-related information, but its acquisition is also complicated and needs multimodal assistance. Hence, in this research a unique approach based on multimodal characterization of the easy-to-acquire Photoplethysmogram (PPG) signal is proposed to assess the stressed condition. Exclusively, the developed algorithm not only uses a primary PPG feature, but also derives the respiratory rate from the same PPG signal via simplified methodologies. The technique is evaluated on the PPG recordings collected from the publicly available DEAP dataset. Efficiency of these easy-to-compute features is then assessed via a simple threshold-based classification technique to categorize the stressed and the relaxed conditions with an average accuracy of 98.43%. Compared to the existing methods, the proposed algorithm not only shows improved performance but the associated simple methodology with minimum acquisition load also justifies its applicability in real-time standalone, personal healthcare applications.
Molecular structures, chemical descriptors and pancreatic lipase (1LPB) inhibition by natural products: A DFT investigation and molecular docking prediction
Authors: Allal Hamza , Nemdili Hacene , Zerizer Mohamed Amine , Zouchoune Bachir 
Published: February 2023

Abstract
Density functional theory (DFT) calculations and molecular docking have been carried out on natural products containing eugenol, ginger, ascorbic acid, oleurpoein, piperine, hesperidin, quercetin, luteonil and curcumin in order to predict their biological activities and to analyse their pancreatic lipase inhibition. The biological activity predictions are based on the global and local chemical descriptors, namely, HOMO-LUMO gaps, chemical hardness, chemical potential, electrophilicity, dipole moment and Fukui functions. Our findings show that the studied compounds can be divided into two groups based on the chemical descriptors, one composed of those of low chemical descriptors, namely, eugenol, ginger, ascorbic acid and oleuropein and the second corresponds composed of piperine, hesperidin, quercetin, luteonil and curcumin in agreement with large HOMO-LUMO gaps and low electrophilicity for the former and conversely for the latter suggesting numerous and interesting biological activities. The frontier orbitals offer a deeper insight concerning the electron-donor and electron-acceptor capabilities, whereas, the local descriptors resulting from Fukui functions put emphasis on the active sites of different candidate ligands. The molecular docking was performed in order to compare and identify the inhibition activity of the natural candidate ligands against pancreatic lipase which were compared to that of synthesized ones. The molecular docking results revealed that the Luteonil compound has the best binding affinity of -8.56 kcal/mol due to their unique molecular structure and the position of -OH aromatic substituents.