bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stereoselective Synthesis of Functionalized 1,3‐Enynes through Recyclable Copper(I)‐Catalyzed Three‐Component Reaction of Terminal Alkynes, Diazoesters and Aldehydes
Authors: Ye Qian , Liao Yang , Luo Chengkai , Cai Mingzhong 
Published: December 2022

AbstractA highly efficient heterogeneous copper(I)‐catalyzed three‐component coupling of terminal alkynes, diazoesters and aldehydes has been achieved by using 10 mol% of copper(I) iodide complex [N,N‐CuI‐MCM‐41] anchored on 2‐aminoethylamino‐modified mesoporous material MCM‐41 as the catalyst under mild conditions, delivering a wide variety of 2‐alkoxycarbonyl‐substituted (E)‐1,3‐enynes in mostly good to high yields with excellent stereoselectivity. The heterogenized copper(I) complex can be facilely prepared from inexpensive reagents by using a simple procedure and exhibits a remarkably higher catalytic activity than CuI, and can be recycled more than ten times without a significant drop in its catalytic efficiency. This protocol represents the first example of heterogeneous copper‐catalyzed stereoselective construction of functionalized 1,3‐enynes from simple and commercially available starting materials.
Accumulation of long‐term diseases is associated with musculoskeletal pain dimensions among middle‐aged individuals with musculoskeletal pain
Authors: Heikkala Eveliina , Oura Petteri , Ho Emma , Ferreira Paulo , Paananen Markus , Karppinen Jaro 
Published: December 2022

AbstractBackgroundLong‐term diseases often co‐occur with musculoskeletal (MSK) pain. In middle‐aged individuals with MSK pain, it remains unclear whether an accumulation (two or more) of long‐term diseases is associated with MSK pain dimensions, including pain frequency, bothersomeness of pain, pain intensity and number of pain sites.MethodsThis cross‐sectional study included data from the Northern Finland Birth Cohort 1966 collected in 2012–2014 when the participants were 46 years of age. We included participants who reported having MSK pain during the previous year (collected retrospectively) and provided self‐reported information related to MSK pain dimensions, long‐term diseases and potential confounders (n = 4469). The association between long‐term diseases and pain dimensions was modelled by general linear and logistic regression models, with beta (β) coefficients, odds ratios (ORs) and their 95% confidence intervals (CIs) being presented. Unadjusted models were followed by models adjusted for sex, educational level and smoking.ResultsThe presence of accumulated long‐term diseases was associated with over two‐fold higher odds of daily pain (adjusted OR 2.6, 95% CI 2.0–3.4) and significantly higher levels of bothersomeness of pain and pain intensity (adjusted β 1.1, 95% CI 0.9–1.4; adjusted β 1.0, 95% CI 0.8–1.1, respectively), relative to the absence of long‐term diseases. Females with accumulated long‐term diseases had a stronger relationship to number of pain sites than males. Associations between one long‐term disease and pain dimensions were significant but smaller in magnitude.ConclusionThere is a need for a better understanding of the relationships between accumulated long‐term diseases and MSK pain.SignificanceThis study on middle‐aged individuals with musculoskeletal pain showed that the presence of long‐term diseases was clearly associated with pain frequency, bothersomeness of pain, pain intensity and number of pain sites. Compared with no long‐term diseases, the association between accumulated (two or more) long‐term diseases and pain dimensions was stronger than the association between one long‐term disease and pain dimensions.
Development of a low‐cost hydrogel microextrusion printer based on a Kossel delta 3D printer platform
Authors: Fanucci Sidne , Prinsloo Earl 
Published: December 2022

AbstractThe core of bioprinting related research aims to reduce the gap between ex vivo cell cultures and in vivo cellular tissue models to further its application within the biomedical field. While additive manufacturing is touted as disruptive technology, bioprinter equipment costs exceed limited resource budgets of many research laboratories restricting the scope for further development for biomedical research and potential medical application. In line with this, a relatively low‐cost bioprinter (SidneV1, SV1) was successfully designed and manufactured using a low‐cost, commercially available FDM Delta 3D printer as a prototype base with a successfully custom designed and manufactured micro‐extrusion printhead. Printing accuracies assessed were 65% (for width measurements) and 64% (for height measurements). This study aimed to demonstrate a way to achieve low‐cost cell‐free hydrogel printing and establish the basis for future system modifications and refinements such that this technology becomes more accessible to under‐funded research groups in resource limited laboratories globally. Optimization of printing parameters, hydrogel and formulations and sterilization techniques will allow for the patterning and engineering of three‐dimensional macroscale scaffolds using this low‐cost printing technology.
circPVT1andPVT1/AKT3show a role in cell proliferation, apoptosis, and tumor subtype‐definition in small cell lung cancer
Authors: Tolomeo Doron , Traversa Debora , Venuto Santina , Ebbesen Karoline K. , García Rodríguez Juan L. , Tamma Grazia , Ranieri Marianna , Simonetti Giorgia , Ghetti Martina , Paganelli Matteo , Visci Grazia , Liso Arcangelo , Kok Klaas , Muscarella Lucia Anna , Fabrizio Federico Pio , Frassanito Maria Antonia , Lamanuzzi Aurelia , Saltarella Ilaria , Solimando Antonio Giovanni , Fatica Alessandro , Ianniello Zaira , Marsano René Massimiliano , Palazzo Antonio , Azzariti Amalia , Longo Vito , Tommasi Stefania , Galetta Domenico , Catino Annamaria , Zito Alfredo , Mazza Tommaso , Napoli Alessandro , Martinelli Giovanni , Kjems Jørgen , Kristensen Lasse Sommer , Vacca Angelo , Storlazzi Clelia Tiziana 
Published: December 2022

AbstractSmall cell lung cancer (SCLC) is treated as a homogeneous disease, although the expression of NEUROD1, ASCL1, POU2F3, and YAP1 identifies distinct molecular subtypes. TheMYConcogene, amplified in SCLC, was recently shown to act as a lineage‐specific factor to associate subtypes with histological classes. Indeed,MYC‐driven SCLCs show a distinct metabolic profile and drug sensitivity. To disentangle their molecular features, we focused on the co‐amplifiedPVT1, frequently overexpressed and originating circular (circRNA) and chimeric RNAs. We analyzed hsa_circ_0001821 (circPVT1) andPVT1/AKT3(chimPVT1) as examples of such transcripts, respectively, to unveil their tumorigenic contribution to SCLC. In detail, circPVT1 activated a pro‐proliferative and anti‐apoptotic program when over‐expressed in lung cells, and knockdown of chimPVT1 induced a decrease in cell growth and an increase of apoptosis in SCLC in vitro. Moreover, the investigatedPVT1transcripts underlined a functional connection between MYC and YAP1/POU2F3, suggesting that they contribute to the transcriptional landscape associated withMYCamplification. In conclusion, we have uncovered a functional role of circular and chimericPVT1transcripts in SCLC; these entities may prove useful as novel biomarkers inMYC‐amplified tumors.
Intrathyroidal parathyroid adenomas: Scoping review on clinical presentation, preoperative localization, and surgical treatment
Authors: Gowrishankar Shravan V. , Bidaye Rohan , Das Tilak , Majcher Veronika , Fish Brian , Casey Ruth , Masterson Liam 
Published: December 2022

AbstractIntrathyroidal parathyroid adenomas (IPAs) are a rare cause of primary hyperparathyroidism. They are often difficult to localize preoperatively and intraoperatively, making diagnosis and treatment challenging. Current data on IPAs are sparse and fragmented in the literature. This makes it difficult to compare the effectiveness of different imaging and surgical techniques. To address this issue, this scoping review maps the literature on IPAs, focusing on four domains: clinical presentation, current localization methods, different surgical techniques, and histopathological features. A search of MEDLINE, Embase, and the Cochrane Library was conducted, with 19 studies meeting the inclusion criteria. The characteristics of IPAs on ultrasound, fine‐needle aspiration, CT, MRI, sestamibi‐based techniques, and selective venous sampling are summarized. Emerging imaging modalities, including autofluorescence, are introduced. Surgical methods and intraoperative factors that correlate with high success rates for removal are highlighted. This review also identifies gaps in knowledge to guide further research into this area.
Chemotherapy in advanced pancreatic adenosquamous carcinoma: A retrospective multicenter AGEO study
Authors: Auvray Kuentz Marie , Hautefeuille Vincent , de Mestier Louis , Coutzac Clélia , Lecomte Thierry , Nardon Victor , Artru Pascal , Turpin Anthony , Drouillard Antoine , Malka David , Tran‐Minh My‐Linh , Trouilloud Isabelle , Lièvre Astrid , Williet Nicolas , Pernot Simon , Touchefeu Yann , Taieb Julien , Hammel Pascal , Zaanan Aziz 
Published: December 2022

AbstractPancreatic adenosquamous carcinoma (PASC) account for <5% of pancreatic malignancies. The efficacy of modern chemotherapy regimens in patients with advanced PASC is unknown. Patients with advanced PASC from 2008 to 2021 were consecutively included in this retrospective multicenter study. Overall survival (OS) and progression‐free survival (PFS) were evaluated by Kaplan‐Meier method. Ninety‐four PASC from 16 French centers were included (median age, 67.3 years; males, 56.4%; metastatic disease, 85.1%). The first‐line treatment was chemotherapy for 79 patients (84.0%) (37 FOLFIRINOX (FX), 7 Gemcitabine‐nab paclitaxel (GN) and 35 for all other regimen) or best supportive care (BSC) alone for 15 patients (16.0%). No significant difference was observed between FX and GN in terms of PFS (P = .67) or OS (P = .5). Modern regimens pooled together (FX and GN) as compared to all others chemotherapy regimens showed an improvement of overall response rate (39.5% and 9.7%, P = .002), PFS (median, 7.8 vs 4.7 months, P = .02) and OS (median, 12.7 vs 9.2 months, P = .35). This large study evaluating first‐line treatment regimens in advanced PASC suggests that modern regimens as FX or GN may be preferable to all other chemotherapy regimens. These results deserve confirmation in prospective studies.
Incidence and mortality trends in gastric cancer in the United States, 1992‐2019
Authors: Chen Mengding , Chen Ke , Hou Huanan , Li Wanjing , Wang Xiaoshan , Dao Qianze , Wang Zhengguang 
Published: December 2022

AbstractOur study aimed to estimate the epidemiological trends of gastric cancer in the United States from 1992 to 2019. This population‐based study used the US Surveillance, Epidemiology and End Results‐12 database as a fundamental cohort to analyze gastric cancer incidence, incidence‐based mortality (IBM), overall survival (OS) and cancer‐specific survival (CSS) probabilities from 1992 to 2019. The Global Burden of Disease study (1990‐2018) was used as a likely validation cohort. Age‐period‐cohort analyses were performed to explore the underlying causes of trend changes. We found that the incidence rate of gastric cancer decreased from 1992 to 2019. IBM also decreased significantly from 1997 to 2019. The 3‐year OS and CSS of gastric cancer increased from 22.3% to 28.7% and 25.7% to 33.5%, respectively. However, the proportion of distant gastric cancer cases had unexpectedly increased rapidly from 33.1% in 1992 to 44.7% in 2019. Age‐period‐cohort modeling found that the incidence and IBM rates remained stable in the groups aged below 50 years, while that in all age groups older than 50 years showed a significant downward trend. High incidence and mortality risks were observed in the younger birth cohorts (birth year after 1990). To conclude, we observed a decline in incidence and mortality rates of gastric cancer in the United States in the past decades. We determined that progression of primary and tertiary preventive measures is the main reason for the reduction in the disease burden of gastric cancer. However, secondary preventive measures for gastric cancer still need to be strengthened.
Advanced strategic constructions of diketopyrrolopyrrole derivatives‐based organic semiconducting phototheranostics
Authors: Wang Bo , Hu Xiaoming , Sun Fengwei , Yang Zhen , Huang Wei 
Published: December 2022

AbstractPhototheranostics as an advanced approach involved in exogenous light‐mediated diagnosis and treatment techniques has attracted broad attention in interdisciplinary biomedical applications. Over the years, deep‐tissue and high‐resolution imaging techniques, such as second near‐infrared (NIR‐II, 1000–1700 nm) fluorescence imaging and photoacoustic imaging, are deemed to be dramatically promising diagnostic approaches. In addition, photonic relevant treatments containing photothermal and photodynamic therapy show great superiorities owing to its high spatiotemporal selectivity and minimal invasiveness. Due to the unique chemical and photophysical properties of diketopyrrolopyrrole (DPP), its derivatives are studied for precision phototheranostics. In this review, the recent progress of DPP agents in the molecular design and biological application for advanced phototheranostics was highlighted, focusing on advanced phototheranostics, such as photoactivated theranostics, tumor microenvironment‐activated theranostics, and hypoxia‐tolerant phototheranostics. In general, this review provides meaningful and directive references for molecular design of phototheranostic platforms in advanced biomedical research.
Boscalid shows increased thyroxin‐glucuronidation in rat but not in human hepatocytes in vitro
Authors: Wiemann Christiane , Melching‐Kollmuss Stephanie , Hambruch Nina , Wiss Lucille , Stauber Franz , Richert Lysiane 
Published: December 2022

AbstractThe fungicide boscalid induces thyroid histopathological and hormone effects in the rat, secondary to liver enzyme induction. To assess the human relevance of liver enzyme induction presumably leading to thyroid hormone disruption, a species comparative in vitro study on T4‐glucuronidation was conducted. Currently, no guidelines how to evaluate Phase II induction are in place. Therefore, we investigated the optimal conditions to evaluate Phase I and Phase II induction potential of boscalid in primary rat (PRH) and human (PHH) hepatocytes. Endpoints included mRNA gene expression and enzyme activities (cytochrome P450 isozymes [CYPs] and uridine diphosphate‐glucuronosyltransferases [UGTs]), measured after 3 (D3) and 7 (D7) days of exposure to reference compounds and to 5, 10, and 20 μM boscalid, focusing on T4‐glucuronidation. Basal CYP activities and T4 glucuronidation were similar or higher on D7 than D3. The highest induction responses of CYPs were on D3, whereas UGT induction and T4‐glucuronidation increases were highest on D7. Boscalid induced CYP1A, CYP2B, and CYP3A mRNA and/or increased related activities in PRH and PHH. Species differences in the induction pattern of UGT genes by reference inducers (ß‐naphthoflavone [BNF], 5‐pregnen‐3ß‐ol‐20‐one‐16α‐carbonitirile [PCN], rifampicin [RIF], and phenobarbital [PB]) and boscalid were seen: UGT1A1, UGT1A3, and UGT1A9 were predominantly induced in PHH, while UGT2B1 was predominantly induced in PRH. Basal activity levels for T4‐glucuronidation were very low in humans and an order of magnitude higher in rat, for this reason increases in activities were assessed as delta activity to the control. Significant increases in T4‐glucuronidation occurred with boscalid in rat but not in human hepatocytes.
The G protein‐coupled receptor ligand apelin‐13 ameliorates skeletal muscle atrophy induced by chronic kidney disease
Authors: Enoki Yuki , Nagai Tomoya , Hamamura Yuna , Osa Sumika , Nakamura Hideaki , Taguchi Kazuaki , Watanabe Hiroshi , Maruyama Toru , Matsumoto Kazuaki 
Published: December 2022

AbstractBackgroundTargeting of the apelin–apelin receptor (Apj) system may serve as a useful therapeutic intervention for the management of chronic kidney disease (CKD)‐induced skeletal muscle atrophy. We investigated the roles and efficacy of the apelin–Apj system in CKD‐induced skeletal muscle atrophy.MethodsThe 5/6‐nephrectomized mice were used as CKD models. AST‐120, a charcoal adsorbent of uraemic toxins (8 w/w% in diet), or apelin (1 μmol/kg) was administered to CKD mice to investigate the mechanism and therapeutic potential of apelin on CKD‐induced skeletal muscle atrophy. The effect of indoxyl sulfate, a uraemic toxin, or apelin on skeletal muscle atrophy was evaluated using mouse myoblast cells (C2C12 cells) in vitro.ResultsSkeletal muscle atrophy developed over time following nephrectomy at 12 weeks, as confirmed by a significant increase of atrogin‐1 and myostatin mRNA expression in the gastrocnemius (GA) muscle and a decrease of lower limb skeletal muscle weight (P < 0.05, 0.01 and 0.05, respectively). Apelin expression in GA muscle was significantly decreased (P < 0.05) and elabela, another Apj endogenous ligand, tended to show a non‐significant decrease at 12 weeks after nephrectomy. Administration of AST‐120 inhibited the decline of muscle weight and increase of atrogin‐1 and myostatin expression. Apelin and elabela expression was slightly improved by AST‐120 administration but Apj expression was not, suggesting the involvement of uraemic toxins in endogenous Apj ligand expression. The administration of apelin at 1.0 μmol/kg for 4 weeks to CKD mice suppressed the increase of atrogin‐1 and myostatin, increased apelin and Apj mRNA expression at 30 min after apelin administration and significantly ameliorated weight loss and a decrease of the cross‐sectional area of hindlimb skeletal muscle.ConclusionsThis study demonstrated for the first time the association of the Apj endogenous ligand–uraemic toxin axis with skeletal muscle atrophy in CKD and the utility of therapeutic targeting of the apelin–Apj system.
Developing transdisciplinarity in first‐year engineering
Authors: Tembrevilla Gerald , Nesbit Susan , Ellis Naoko , Ostafichuk Peter 
Published: December 2022

AbstractBackgroundFor engineers who aim to address sustainability challenges, participating in transdisciplinary teams is key. Yet developing transdisciplinary knowledge, including systems thinking, metacognition, and empathic thinking, is not well supported in traditional engineering programs.PurposeThe extent to which selected learning activities in the introduction to engineering courses support student development of systems thinking, metacognition, and empathic thinking is investigated.Design/MethodFocus group discussions with instructional teams and student interviews are examined to elucidate how course activities improved student transdisciplinary knowledge. Threshold concepts frame the qualitative analysis of the collected data. Implications for teaching and learning are discussed.FindingsResults suggest the investigated learning activities support student development of transdisciplinary knowledge as indicated by changes in systems thinking, metacognition, and empathic thinking. Where prior quantitative exploratory studies revealed little change in transdisciplinary knowledge indicators pre‐ and post‐course, deeper qualitative analysis uncovers students manifested improvements in transdisciplinary knowledge indicators as narrated by the students themselves and as observed by instructors and teaching assistants.ConclusionsIntegrating transdisciplinary knowledge development into engineering programs, starting with appropriate learning activities in first‐year engineering courses, may provide new pathways for transforming curricula aimed at educating the 21st‐century engineer.
Uptake, acceptability and interpretability of 3‐in‐1 rapid blood self‐testing for HIV, hepatitis B and hepatitis C
Authors: Salvadori Nicolas , Achalapong Jullapong , Boontan Chonlatorn , Piriya Choosakun , Arunothong Surachet , Nangola Sawitree , Kloypan Chiraphat , Prompunt Eakkapote , Khamduang Woottichai , Moolnoi Phornphimon , Pornprasert Sakorn , Ongwandee Sumet , Mary Jean Yves , Jourdain Gonzague , Ngo‐Giang‐Huong Nicole 
Published: December 2022

AbstractIntroductionEarly diagnosis is key to achieving the goal of eliminating transmission of HIV and hepatitis B and C. We assessed the uptake, acceptability and interpretability of self‐testing using a 3‐in‐1 rapid diagnostic test (RDT) in facility‐based services.MethodsStand‐alone testing services were provided free of charge to consenting individuals aged ≥15 years in five facilities in northern Thailand. Clients were invited to choose between self‐testing by fingerprick or venepuncture by a healthcare worker (HCW). In each facility, several clients could simultaneously self‐test in separate private areas using TriQuik™ (Genlantis, San Diego, CA, USA), a single immunochromatographic cassette detecting HIV‐1/2 antibody, hepatitis B surface antigen (HBsAg) and hepatitis C antibody (HCAb). An interactive program on a tablet computer was developed to collect socio‐demographic, behavioural and satisfaction data and provide information to guide the self‐test process, including video instructions, results interpretation and a picture of the cassette for immediate remote review by the HCW. When the HCW interpreted an HIV self‐test as positive, the HCW collected blood by venepuncture for immediate confirmation.ResultsBetween October 2020 and April 2022, 4119 clients presented for testing for the first time as part of the project. Of them, 3462 (84.0%) opted for self‐testing. Among self‐testers, 1801 (52.0%) were born female, the median age was 27 years (interquartile range, 22–36), 661 (19.1%) belonged to at least one key population and 2124 (61.4%) had never been tested for HIV; 3329 (99.8% of those who answered) reported being “very satisfied” or “satisfied” with the testing process. The proportions of test results interpreted as positive by self‐testers among those interpreted as positive by HCWs were 95% for HIV‐1/2 antibody, 95% for HBsAg and 78% for HCAb.ConclusionsThese proportions were higher than those observed in a previous study evaluating another 3‐in‐1 RDT for HIV, HBsAg and HCAb, possibly due to the use of video instructions instead of paper‐based instructions, lower prevalence and co‐infection rates, or lower percentages of clients with low education level. Multiplex self‐testing simplified and streamlined the service delivery process and was well accepted. HCW assistance proved to be essential in a limited number of cases.