bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinctive Characteristics and Dynamics of the Summer and Autumn Indian Ocean Dipole Events
Authors: Tao Yuqi , Qiu Chunhua , Zhong Wenxiu , Zhang Guangli , Wang Lin 
Published: May 2023

Abstract
The Indian Ocean Dipole (IOD) with worldwide socio-economic impacts has been presented to mature either in boreal summer or autumn, leading to the classification of summer IOD and autumn IOD. Investigating the climate dynamics to distinguish between these two types of IOD can improve our understanding and prediction of the surrounding weather and climate. This study demonstrates that the emergence of the summer IOD is mainly attributed to internal air-sea interactions in the western tropical Indian Ocean (WIO), while the autumn IOD is significantly related to ENSO development. For the summer IOD, broad-scaled warm sea surface temperature anomalies in the WIO are conducive to the enhancement of convective perturbations. Then local ocean-atmosphere feedback associated with changes in convection and surface heat flux into the upper ocean plays a key role in triggering the summer IOD. For the autumn IOD, strong easterly wind anomalies in the eastern Indian Ocean initiate oceanic Rossby waves and Bjerknes feedback, leading to the formation of both the western and eastern poles. It is recognized that these intensified easterly wind anomalies mostly benefit from ENSO variability. The distinctive features and air-sea interactions intrinsic to the summer IOD and the autumn IOD revealed in this study can further contribute to more credible predictive models of diverse IOD events.
Predicting Bone Protein Expression: A Stiffness-Dependent Gene Regulation Model
Authors: Berteau Jean , Yang Kevin Yueh-Hsun , Chekroun Abdennasser , Pujo-Menjouet laurent 
Published: May 2023

Abstract
This paper aims to capture the behavior of the bone gene regulation network (GRN) portion that controls mineralization according to stiffness. We hypothesized that the epistatic genetic interactions follow a system of nonlinear differential equations. To test our hypothesis, we (i) adapted our previously published model from being continuously time-dependent to continuously stiffness-dependent, (ii) performed in-vitro experiments culturing bone cells on different stiffness, and (iii) optimized our model based on the experimental results. Our results showed that (i) we exhibited a continuous variation of stiffness that allowed us to simulate bone GRN gene expression, and (ii) we qualitatively validated our approach against a novel set of experimental data. Thus, we depict novel mathematical solutions that predict the bone GRN expression regarding stiffness. Therefore, we propose a model of how mechanotransduction controls the epistatic genetic interactions that govern mineralization. Our novel algorithm can predict Bone protein production or matrix stiffness with only one input.
Abnormal hemoglobin anti-Lepore Hong Kong compound with β0-thalassemia ameliorate thalassemia severity when co-inherited with a-thalassemia
Authors: Bao xiuqin , Wang Jicheng , Qin Danqing , Yao Cuize , Liang Jie , Liang Kailing , Du Li 
Published: May 2023

Abstract
Background Abnormal hemoglobin anti-Lepore Hong Kong is rare βδ fusion variants resulted from non-homologous crossover during meiosis. Anti-Lepore Hong Kong always showed extraordinary increased HbA2. In this study, we used multiplex ligation-dependent probe amplification (MLPA) and single molecular real time (SMRT) sequencing, as well as Sanger sequencing, to detect variants in five unrelated families with abnormal increased HbA2 level.Results All the probands in these five families were detected with anti-Lepore Hong Kong heterozygotes. Among them, two families were compounded with β0-thalassemia and α-thalassemia (--SEA/ or αCSα/). The heterozygotes of anti-Lepore Hong Kong had an average 17.7% of HbA2 level and behaved normal. However, when compounded with β0-thalassemia and α-thalassemia, the probands had higher HbA2 level (30.2%-40.8%) and behaved with β-thalassemia trait. Moreover, the determination of α/β-mRNA ratio showed a slightly downregulation of β-globin, similar to that of β-thalassemia minor.Conclusions Our study first identifies the compound heterozygotes with anti-Lepore Hong Kong and β0-thalassemia and α-thalassemia, and provide better information in prenatal counseling.
A Multi-depth Spiral Milli Fluidic Device for Whole Mount Zebrafish Antibody Staining
Authors: Ye Songtao , Chin Wei-Chun , Ni Chih-Wen 
Published: May 2023

Abstract
Whole mount zebrafish antibody staining (ABS) is a common staining technique for protein information localization on a zebrafish embryo or larva. Like most biological assays, the whole mount zebrafish ABS is still largely conducted manually through labor intensive and time-consuming steps which affect both consistency and throughput of the assay. In this work, we develop a milli fluidic device that can automatically trap and immobilize the fixed chorion-less zebrafish embryos for the whole mount ABS. With just a single loading step, the zebrafish embryos can be immobilized in the milli fluidic device through a chaotic hydrodynamic trapping process. Moreover, a consistent body orientation pattern (i.e., head point inward) for the trapped zebrafish embryos can be achieved without any additional orientation adjustment device. Furthermore, we employed a consumer-grade SLA 3D printer assisted method for device prototyping which is ideal for labs with limited budgets. Notably, the milli fluidic device has enabled the optimization and successful implementation of whole mount zebrafish Caspase-3 ABS. We demonstrated our device can accelerate the overall procedure by reducing at least 50% of washing time in the standard well-plate-based manual procedure. Also, the consistency is improved, and manual steps are reduced using the milli fluidic device. This work fills the gap in the milli fluidic application for whole mount zebrafish immunohistochemistry. We hope the device can be accepted by the zebrafish community and be used for other types of whole mount zebrafish ABS procedures or expanded to more complicated in situ hybridization (ISH) procedure.
Household-level forest-community involvement in the buffer area of Biha Resort, Bukit Barisan Selatan National Park, Indonesia
Authors: Mardiyanto Anggi , Hidayat Ar. Rohman Taufiq , Sianipar Corinthias Pamatang Morgana , Shibata Shozo 
Published: May 2023

Abstract
Biha Resort in Bukit Barisan Selatan National Park (BBSNP) is an essential habitat for protected species such as the Sumatran tiger, the Sumatran elephant and the Sumatran rhino, which exhibit declining population trends, and also provides a living for local communities. However, research on the characteristics of such communities (including factors such as involvement with multiple forest types for the output of various products) has to date been sparse. Against such a background, this study was conducted to clarify the characteristics of rural households around the buffer area of Biha Resort and their involvement relating to forest products in multiple forest types. Qualitative research was conducted via a semi-structured questionnaire survey involving 128 households, with results detailing activities in customary forests, limited production forests and BBSNP for the output of various forest products, with customary forests being the most utilized. The outcomes showed that forest products are directly consumed and used to generate income, with food being the most frequently used resource. In addition, traditional forest management practices such as repong damar (traditional ecological knowledge in local agriculture) are used to provide resources for the community. The results provide a foundation for efforts to balance biodiversity conservation and forest community welfare.
Effectiveness of the physical intervention using sensory integration theory in the recovery of children with autism
Authors: Wang Qiang , Xu Lei 
Published: May 2023

Abstract
In human body, sensory integration plays a significant role as it is a mutually reinforcing and complementary process of the body in general and human brains in particular. This process extensively utilizes the nervous system during individual development for the creation of numerous sensations, which are very helpful in making people act accordingly. It is important to note that body and brain will not be able to perform in unison preferably without sensory integration. This is one of the most challenging issues related to the people especially with autism face. Generally, number of children suffering with autism is increasing day by day at a predominant rate, however, the exact cause of these remains unknown until today. Furthermore, as per our knowledge, positive outcomes or opinions on its onset are not reported yet in literature, which is very alarming, and currently special care behavior training is suggested to be taken by the individuals. By thoroughly analyzing the literature combined with the special psychological characteristics of autistic children, we have observed that sports is one of the possible ways which could possibly help these children in the development process of their brains and bodies, but a necessary measure to intervene and improve their conditions are needed to be adopted as well. In order to solve the problem of sensory integration disorder in children with autism, this paper proposes an effective mechanism for determining the effectiveness of the physical intervention using sensory integration theory on the recovery of children with autism. To investigate this, we have started with sensory integration theory, which is one the most vital factor in the in recovery process of children suffering from the autism. Additionally, it takes a unique approach to designing physical activity for children with autism, and builds a model of physical play assessment followed by an intervention experiment. In order to verify various claims of the proposed scheme, we have carried out numerous experimental studies which conclude that the proposed approach is affective mechanism for solving the problem.
Glycerol biosynthetic pathway plays an essential role in proliferation and antioxidative defense in the human enteric protozoan parasite Entamoeba histolytica
Authors: Jeelani Ghulam , Balogun Emmanuel Oluwadare , Husain Afzal , Nozaki Tomoyoshi 
Published: May 2023

Abstract
Amebiasis is caused by the protozoan parasite Entamoeba histolytica. Treatment options other than metronidazole and its derivatives are few, and their low efficacy against asymptomatic cyst carriers, and experimental evidence of resistance in vitro justify the discovery/repurposing campaign for new drugs against amebiasis. Global metabolic responses to oxidative stress and cysteine deprivation by E. histolytica revealed glycerol metabolism may represent a rational target for drug development. In this study using 14C-labelled glucose, only 11% of the total glucose taken up by E. histolytica trophozoites is incorporated to lipids. To better understand the role of glycerol metabolism in this parasite, we characterized two key enzymes, glycerol kinase (GK) and glycerol 3-phosphate dehydrogenase (G3PDH). E. histolytica GK revealed novel characteristics and unprecedented kinetic properties in reverse reaction. Gene silencing revealed that GK is essential for optimum growth, whereas G3PDH is not. Gene silencing of G3PDH caused upregulated GK expression, while that of GK resulted in upregulation of antioxidant enzymes as shown by RNA-seq analysis. Together, these results provide the first direct evidence of the biological importance and coordinated regulation of the glycerol metabolic pathways for proliferation and antioxidative defense in E. histolytica, justifying the exploitation of these enzymes as future drug targets.
Impact of Estrogen Replacement Therapy on Hypogonadal Survivors of Childhood Cancer
Authors: Nakamura Miwa , Ohba Takashi , Sasaki Rumi , Saito Fumitaka , Yamaguchi Munekage , Motohara Takeshi , Mabe Hiroyo , Lu Xi , Katabuchi Hidetaka , Kondoh Eiji 
Published: May 2023

AbstractBackground:Hypogonadism is a significant late complication in childhood cancer survivors (CCS). The aim of this study was to elucidate the benefits and limitations of estrogen replacement therapy (ERT) for CCS with hypogonadism.Methods:Seventeen CCS were divided into two groups: gonadal hypogonadism (GH) group (n=8) and central hypogonadism (CH) group (n=9). Pearson correlation coefficients were used to investigate the impact of cancer management on final height, bone density, and uterine development.Results:Seven of GH group had hematologic malignancies, and all of them underwent total body irradiation before bone marrow transplantation. The GH group showed significant positive correlations between the onset age of disease treatment and final height (p < 0.05, R = 0.712) and uterine size following ERT (p < 0.05, R = 0.775). All CCS in the CH group had brain tumors, and seven of them received chemotherapy. There were trends towards positive and negative correlations between the onset age of disease treatment and final height (p = 0.09, R = 0.527) or uterine size (p = 0.07, R = -0.669), respectively. A negative correlation trend was observed between the age at ERT initiation and final height (p = 0.07, R = -0.769) or bone density (p = 0.18, R = -0.626) in six CH patients who received growth hormone therapy. Five CCS in both groups experienced osteoporosis, despite receiving ERT.Conclusion:Individualized management strategies beyond ERT are essential to reduce long-term complications in CCSs with hypogonadism, considering the type and timing of cancer treatment.
Ocular Injuries Associated with Motor Vehicle Accidents - Long Term Effects on Quality of Life
Authors: Brody Judith , Ishai Meydan Ben , Volach Irena Serov , Tamir Keren Mano , Gaton Dan D. , Avisar Inbal 
Published: May 2023

Abstract
Purpose To describe the prevalence and outcome of motor vehicle accidents - associated ocular injuries.Methods A survey of patients who presented to the emergency room at a level 1 trauma center with motor vehicle accidents -associated ocular injuries. A patient questionnaire and review of clinical notes were conducted for all patients.Results Of 274 motor vehicle accident victims with ocular injuries who presented to the emergency room, 40 (15%) responded to the survey. Over half of them were driving a vehicle, and most reported wearing a seat belt or a helmet. Most ocular injuries were mild. The most common injuries were bone fractures, subconjunctival hemorrhage, eyelid involvement and corneal injury. Most respondents had no change in vision and perceived their ocular involvement as a minor part of their injury. Most respondents returned to work and to driving within a year.Conclusion Our study sheds light on the details and extent of ocular involvement and the visual ability to perform daily activities following motor vehicle accidents.
Problem of integrating Blockchain Technologies into Wireless Mesh Networks: Application to Community Wireless Networks
Authors: NDIÉ Thomas DJOTIO , DOBE Abel 
Published: May 2023

Abstract
Wireless mesh networks (WMNs), because of their low installation costs, compared to the network infrastructure, were seen as a relevant alternative to connect remote areas and poor regions where telephone companies have great apprehension to invest. However, WMNs have security vulnerabilities, including Denial of Service (DoS) and identity theft attacks. The most common methods for securing mesh networks are centralized systems, while WMNs are decentralized, open and flexible by design. The usual blockchain integration in WMNs are more oriented towards the construction of online payment platforms. This paper proposes BlockWMN, a model for integrating blockchain technology into WMNs with the ultimate goal of protecting them from the above-mentioned attacks. Fundamentally we address a consensus issue or a so-called "Byzantine Generals" or "Byzantine Fault Tolerance" (BFT) problem. Our approach uses the blockchain as a secure database, to save in real time the network graph and all the network nodes’ credentials. Each node has the ability to check in the blockchain, all signaling information received from other nodes. Unlike other approaches, our solution is decentralized, open and flexible. The limits of our model are inherent to the blockchains nature: high-energy consumption for the calculation of the proof of work and memory space for the storage of blockchain information.
Wood Decay Measurement of Shiitake Cultivation Logs Based on Spin Relaxation Times Obtained using Magnetic Resonance Imaging
Authors: Ogawa Kuniyasu , Yashima Takeshi 
Published: May 2023

Abstract
To harvest large shiitake mushrooms (Lentinula edodes), it is important to adjust the suitable cultivation environment for shiitake mycelium so that it can extend throughout an entire log, maximizing wood decomposition and nutrient absorption. We attempted to measure the degree of wood decay of cultivation logs noninvasively by Magnetic Resonance Imaging (MRI). The cultivation logs were sourced from konara oak (Quercus serrata), which was inoculated with shiitake mycelium for 0.6 to 4 years. After MRI measurements, the logs were cut into wood pieces between 1 to 2 cm in length, and the degree of wood decay of the individual pieces was evaluated using the dry wood density. NMR signals were measured using a long echo time, TE = 11 ms, to obtain T1 and T2 relaxation times of free water in the logs. The results showed that wood decay of the cultivation logs correlated well with T2 values and poorly with T1 values. In addition, based on the obtained T2 map, the spatial distribution of wood decay within cultivation logs 1.6 years after inoculation could be visualized.
Flowering and fruit-set depend of interactions between climate and soil texture in Amazonian understorey herbs
Authors: Rodrigues Flávio Rogério de Oliveira , Freitas Cintia Gomes , Dambros Cristian 
Published: May 2023

Abstract
The persistence of a plant at an area depend on species ability to survive and reproduce under local conditions. Estimating environmental influence on reproduction is difficult because climatic and soil conditions have synergistic effects on flower and fruit production, but are essential to modeling correctely effects of the climate changes on species distributions. Here, we develop a statistical method that allows investigating the environmental influence on reproduction using reproduction observational data only (no individual plants marking required). We surveyed 30 standardized permanent plots on central Amazonia for six families of monocots herbs. The plots were surveyed for twelve consecutive months. Using the newly developed method, we estimated the effect of climatic (temporal) and edaphic (spatial) covariates on flower and fruit production. We demonstrate that plant reproduction can be estimated from observational data only when enough temporal and spatial data are available. By using the proposed statistical method, we show that the conversion of flowers into fruits in Amazonian monocots is highest in sandy soils, and the difference in conversion rates between sandy and clayey soils is more pronounced in wetter months. By comparing the production of flowers and fruits with previously published data on species occurrence, our results suggesting that species distribution is limited primarily by species capacity to produce fruits (e.g. limited pollination) and not by the capacity to produce flowers. Due to the association of fruit production to climatic and edaphic variable, our results point to potential changes in species reproduction and distribution under future climatic regimes.
A Structure and Spectroscopy study about [16]Cycloparaphenylene Chiral Molecule
Authors: Yang Baozhu , Huang Shuang 
Published: May 2023

Abstract
An interesting chiral molecule with a double half-twisted π-electron system has been investigated with theoretical calculations. To investigate the geometry and electronic structure, the size of macrocyclic cavity, electrostatic potential (ESP) and density-of-states (DOS) were calculated. The multi-center bond order (MCBO) and AV1245 index were calculated to compare the aromaticity of phenylene groups. To investigate the spectroscopic properties, Raman, Raman Optical Activity (ROA), UV-Vis spectrum, fluorescence, CPL (circularly polarized luminescence) and ECD (electronic circular dichroism) spectra have been calculated and analyzed. The oscillator strengths, rotatory strengths and dissymmetry factor (glum) have been discussed.
Model Construction and Verification of Sprinkler Pattern Radius Calculation
Authors: Tuo Yunfei , Tan Hao , Liang Jiaping , Liu Xiaogang , Xiang Ping , Yang Qiliang , Li Jianqiang , He Xiahong 
Published: May 2023

Abstract
To determine Calculation method of sprinkler and micro-sprinklers, computational models of sprinkler pattern radius model is established which based on hydraulic parameters of nozzle diameter, working pressure, Installation Heights, spray elevation angle, discharge coefficient, wind direction and speed, water flow regime, and water droplet motion in air, etc., as well by Newton's second law of motion, aerodynamics, and fluid mechanics principles, and by experimental verification for common sprinkler pattern radius, nozzle diameter, working pressure, spray elevation angle, Installation Heights, and wind direction and speed. The results indicate that sprinkler pattern radius with nozzle diameter, working pressure, Installation Heights, spray elevation angle, discharge coefficient, wind direction and speed, water flow regime, and droplet motion in air was in highly significant correlation, the sprinkler pattern radius Progressive decreases around the sprinkler along the sprinkler pattern radius direction, as well increases by 5.2342%~10.6278% in the downwind direction and decreases by 4.6897%~9.8623% in the headwind direction compared with no wind. The wind speed increases, then the range decreases, and the wind direction shifts, then the range shifts, so the wind direction wind speed changes then the range changes. The coefficients of determination, the consistency Index, the root mean square error, absolute error, relative error between calculated Values and Measured Values of computational models are 0.9146, 0.9205, 8.7748%, 10.4980%, 8.2524% and 8.1421%, respectively. The smaller coefficients of determination and the consistency Index of calculated Values and Measured Values of computational model under windy conditions comparing with no wind, as well the larger root error, deviation, absolute error and relative error. The calculation model in this paper has a clear principle, comprehensive influence factors, complete physical meaning, high calculation accuracy, small error, and wide applicability. This calculation model can effectively reflect the nozzle range change law. This study provides theoretical basis for determining the sprinkler hydraulic performance and provides technology support for Planning and Design of sprinkler irrigation system.