bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fatty acids abrogate the growth-suppressive effects induced by inhibition of cholesterol flux in pancreatic cancer cells
Authors: Li Yuchuan , Amrutkar Manoj , Finstadsveen Anette Vefferstad , Dalen Knut Tomas , Verbeke Caroline S , Gladhaug Ivar P. 
Published: July 2023

Abstract
Background
Despite therapeutic advances, the prognosis of pancreatic ductal adenocarcinoma (PDAC) remains extremely poor. Metabolic reprogramming is increasingly recognized as a key contributor to tumor progression and therapy resistance in PDAC. One of the main metabolic changes essential for tumor growth is altered cholesterol flux. Targeting cholesterol flux appears an attractive therapeutic approach, however, the complex regulation of cholesterol balance in PDAC cells remains poorly understood.
Methods
The lipid content in human pancreatic duct epithelial (HPDE) cells and human PDAC cell lines (BxPC-3, MIA PaCa-2, and PANC-1) was determined. Cells exposed to eight different inhibitors targeting different regulators of lipid flux, in the presence or absence of oleic acid (OA) stimulation were assessed for changes in viability, proliferation, migration, and invasion. Intracellular content and distribution of cholesterol was assessed. Lastly, proteome profiling of PANC-1 exposed to the SOAT1 inhibitor avasimibe, in presence or absence of OA, was performed.
Results
PDAC cells contain more free cholesterol but less cholesteryl esters and lipid droplets than HPDE cells. Exposure to different lipid flux inhibitors increased cell death and suppressed proliferation, with different efficiency in the tested PDAC cell lines. Avasimibe had the strongest ability to suppress proliferation across the three PDAC cell lines. All inhibitors showing cell suppressive effect disturbed intracellular cholesterol flux and increased cholesterol aggregation. OA improved overall cholesterol balance, reduced free cholesterol aggregation, and reversed cell death induced by the inhibitors. Treatment with avasimibe changed the cellular proteome substantially, mainly for proteins related to biosynthesis and metabolism of lipids and fatty acids, apoptosis, and cell adhesion. Most of these changes were restored by OA.
Conclusions
The study reveals that disturbing the cholesterol flux by inhibiting the actions of its key regulators can yield growth suppressive effects on PDAC cells. The presence of fatty acids restores intracellular cholesterol balance and abrogates the alternations induced by cholesterol flux inhibitors. Taken together, targeting cholesterol flux might be an attractive strategy to develop new therapeutics against PDAC. However, the impact of fatty acids in the tumor microenvironment must be taken into consideration.
The Impact of the Right Heart Cavities Echocardiographic Features on Atrial-High Rate Episodes
Authors: ARTAC Inanc , KARAKAYALI Muammer , OMAR Timor , ILIS Dogan , ARSLAN Ayca , Sahin Mehmet Hakan , KARABAG Yavuz , RENCUZOGULLARI Ibrahim 
Published: July 2023

Abstract
Objectives
Atrial high-rate episodes (AHRE) defined as atrial tachy-arrhythmias, detected through continuous monitoring with a cardiac implantable electronic device (CIED). AHRE's have been associated with increased risks of developing clinically manifested atrial fibrillation (AF), thromboembolism, cardiovascular events, and mortality. Several variables have been researched and identified to predict AHRE existence. The present study evaluated the association between right-heart structural and functional echocardiographic parameters and AHRE in patients with CIEDs.
Materials and Methods
This prospective design study included 194 patients with CIED's. The study population was divided into two groups according to presence of AHRE and analyzed the echocardiographic variables which may able to be a predictor of AHRE.
Results
Patients was divided into two groups: patients with AHRE (+) and without AHRE (-). The distribution of patients’ characteristics according to presence of AHRE was analyzed. The multivariate analysis revealed Age, LAVI, E/Em tricuspid (HR: 1,106, 1,015 − 1,205%95 CI, p = 0,022) and RAVI (HR: 1,035, 1,003 − 1,069 95% CI; p = 0,033) as independent predictors of AHREs. ROC curve analysis indicated that an E/Em tricuspid (AUC: 0,611, 95% CI: 0,538-0,680 p: 0.009) and RAVI (AUC = AUC: 0,707, 95% CI 0,637-0,770 p < 0.001) predicted AHREs with a cut-off value of 6.28 and a sensitivity of 53,2% and specificity of 66,7% and a cut-off value of 29,5 mL/m2 with a sensitivity of 72,7% and a specificity of 65,9%, respectively.
Conclusion
The main finding of this study was "RAVI" and "E/Emtricuspid ratio" is associated with AHRE. Additionally, "RAVI" and "E/Emtricuspid ratio" is an independent predictor of AHRE existence.
Exploring the Attributes of Influential Users in Social Networks using Association Rule Mining
Authors: Alghobiri Mohammed 
Published: July 2023

Abstract
Association rule mining discovers interesting patterns and meaningful connections between items or actions performed by users on social media platforms. These connections can provide valuable insights into user behavior, preferences, and interactions within the social media ecosystem. This study utilizes the association rule mining to identify Key attributes of influential individuals who can effectively influence others to actively participate in activities such as writing posts, answering questions, and sharing posted content on popular social news aggregation and discussion websites such as reddit.com. The research relies on user profiles and activity logs as data sources for analysis. The study's findings include the observation that highly influential sharers often engage in regular content creation and sharing related to topics like entrepreneurship, personal development, and professional growth. Furthermore, it suggests that influential sharers are active during both business and prime times. In terms of specific dimensions of interest, it was found that women are more likely to be influenced by individuals who frequently write about personal growth. Similarly, the study highlights that teenagers have the most influence over their peers. Additionally, when considering the interplay of age and gender, it has been identified that adult males, especially, possess the ability to convince and influence other males. The insights gained from this study can prove valuable to marketers seeking to target specific individuals for effective social marketing campaigns.
Incidence, survival, and prognostic factors for patients with gastrointestinal mixed neuroendocrine non-neuroendocrine neoplasms: a SEER population-based study
Authors: Xu Boqi , Zhang Fan , Wu Runda , Peng Yao , Mao Zhongqi , Tong Shan 
Published: July 2023

Abstract
Background Mixed neuroendocrine non-neuroendocrine neoplasms (MiNENs) are a group of rare and significantly heterogeneous tumors with limited research currently available. This study aimed to analyze the incidence, survival, and prognostic factors of gastrointestinal MiNENs.Methods We selected data from the Surveillance, Epidemiology, and End Results (SEER) database between 2000 and 2019 and evaluated the incidence trend of gastrointestinal MiNENs during this period. We utilized univariate and multivariate Cox analysis to assess independent factors of prognosis and established a nomogram to predict 1-, 2-, and 3-year cancer-specific survival (CSS). Calibration and receiver operating characteristic (ROC) curves were drawn to validate the accuracy and reliability of the model. Decision curve analysis (DCA) was used to assess the clinical utility of the model.Results The overall incidence of gastrointestinal MiNENs has been increasing annually. Multivariate Cox regression analysis revealed that tumor grade, size, lymph node metastasis, distant metastasis, and surgery were independent risk factors for CSS in MiNENs patients. Based on these risk factors, the 1-, 2-, and 3-year CSS nomogram model for MiNENs patients was established. Calibration curves, ROC curves, and DCA of the training and validation sets demonstrated that this model had good accuracy and clinical utility.Conclusion Gastrointestinal MiNENs are rare tumors with an increasing incidence rate. The nomogram model is expected to be an effective tool for personalized prognosis prediction in MiNENs patients, which may benefit clinical decision-making.
Nutritional, anti-nutritional and morphological characterization of wild edible species of Solanum found in North East India
Authors: Ningthoujam Rina , Deo Chandra , Phurailatpam Arunkumar , BN Hazarika , Bhutia Nangsol Dolma , Heisnam Punabati , Mayanglambam Chandrakumar , SINGH YENGKHOM DISCO 
Published: July 2023

Abstract
The outcome of this study shows that S. species are considerably variate in nutritional, anti-nutritional and morphological characters. Maximum plant height (161.16±5.67 cm) and number of flowers per inflorescence (12.49±1.55) was obtained from S. torvum while S. nigrumattained minimum fruit length (6.08±0.43 mm) and girth (6.48±0.55 mm). Among all the berries, S. macrocarpon has wider girth than length. So, it perceived maximal fruit girth (73.65±0.65 mm) and lowest number of flowers per inflorescence (2.04±0.43). Being a short period plant, S. nigrum also recorded least number of days to first flowering (19.94±3.63) and no. of days taken for fruit development (31.27±2.99). Regarding nutritional composition, highest amount of total carbohydrates (47.44±1.75 mg/g) and starch (8.76±0.16 mg/g) was recorded in S. macrocarpon whereas it has least value of cellulose (0.75±0.007 mg/g). S. ferox achieved maximal amount of total sugar (7.33±0.78 mg/g) and reducing sugar (3.86±0.40 mg/g). Likewise, S. spirale recorded minimum amount of total sugar (2.37±0.12 mg/g) and reducing sugar (0.23±0.03 mg/g) while S. torvum obtained highest quantity of cellulose (2.04±0.08 mg/g). Total soluble protein was observed from S. villosum with an amount of 1.54±0.03 mg/g whereas S. gilo (white) obtained lowest 0.74±0.03 mg/g soluble protein. As for anti-nutritional highest quantity of phytic acid was recorded in S. spirale (5.34 ±0.18 mg/g) while least amount was obtained from S. ferox(0.72 ±0.01 mg/g). However, maximal amount of cyanogen i.e., 54.24 ±0.59 µg/g was attained from S. violaceum(spineless) and lowest quantity of cyanogen at 12.72 ±0.56 µg/g was recorded in S. gilo (green).
Data Protection Using Optical System and Steganography Tool
Authors: Patra Anirban , Saha Arijit , Roy Samiran 
Published: July 2023

Abstract
Steganography, which is the art of hiding secret information, is widely used by researchers for the last few decades. However, maximum people use commercially available stenography tools due to their easy availability. In most of these tools, it is quite easy to decrypt hidden information using a different process. Hence, it is a difficult task to securely communicate personal information using this type of steganography tool. In this communication, we propose a novel encryption process of hidden information into the stage images and texts in the optical system (grating). The entire encryption process is divided into two stages. First, the images and texts are hidden into other images using the commercially available steganographic tool, Steghide, and then modulated in the optical grating system. In the second part, the resultant object is further processed by the random phase matrix. We tested in different decryption software and observed that the hidden image and text cannot be successfully decrypted. Therefore. this process can be used effectively to secure hidden information in the steganographic process.
How do Children with Developmental Dyslexia Integrate Sentence-Picture Information?
Authors: Shi Duyuan , Yang Mengting , Yao Ru , Li Tingyu , Zhang Hongxia 
Published: July 2023

Abstract
Children with developmental dyslexia have significant defects in reading, and many researches show that the form of information presentation will have different effects on individual reading efficiency. This study has adopted a mixed design of 2 (subject group: children with developmental dyslexia, typical development children)×3 (sentence style: affirmative, negative, double-negative)×2 (picture information complexity: simple, complex) to investigate the sentence-picture information integration capability of children with developmental dyslexia, and explore whether children with developmental dyslexia have defects in the ability to integrate sentence-picture information. The results showed that: 1. Sentence style had a significant effect on the integration process, and the reading efficiency of both groups decreased with the increase of sentence structure complexity in all three sentence styles; 2. Picture information complexity had a significant effect on the integration process, and both groups were more efficient in integrating sentence-picture in the simple condition than in the complex condition; 3. Children with developmental dyslexia haddefects in the ability to integrate sentence-picture information. Conclusion: For children with developmental dyslexia, simple sentences, such as affirmative sentences with simple picture information without distractions, can be presented as far as possible to improve their reading efficiency.
Experimental Study of 3D Printing Technique Combined with Biphasic Calcium Phosphates in the Treatment of Canine Model of Osteonecrosis of the Femoral Head
Authors: chen zhian , Feng Fanzhe , Su Xixiong , Xu Yongqing , Zhang Ying , Tan Hongbo 
Published: July 2023

Abstract
Objective:This study attempts to use a digital design of 3D printing technology to create a surgical navigation template. At the same time, biphasic calcium phosphates (BCP) were used in the treatment of animal models of osteonecrosis of the femoral head (ONFH) basis on accurate positioning of necrotic lesions in the navigation templates and observing its therapeutic effect.
Methods:15 healthy adult beagle dogs of male and female, weight (20 + 2) kg, after establishing the model of ONFH using liquid nitrogen freezing method, were randomly divided into 3 groups (n=5). Each model underwent necrosis lesions and BPC implantations in one side of femoral head, and only underwent necrosis lesions in one other side of femoral head. Each group underwent CT examination, gross observation,histological examination and immunohistochemical staining at 6 weeks, 12 weeks and 18 weeks postoperatively.
Results:At weeks 6, 12, and 18, CT and gross examination showed that the necrotic area in the experimental group was basically intact and had been completely raised by BCP material. In the control group, there were signs of bone repair in the femoral head, but there were still large bone defects and cavities,At week 18, extensive collapse of the cartilage surface was observed.Through histological examination, in the experimental group at 12 and 18 weeks, a large number of new and reconstructed bone trabeculae containing a large amount of collagen fibers were observed (P<0.05), while in the control group, there was extensive necrosis of the bone trabeculae without cellular structural areas.Immunohistochemical examination observation: A large number of CD31 positive cells were observed in the experimental group at 6 weeks, gradually decreasing at 12 and 18 weeks (P<0.05), while a small number of CD31 positive cells were observed in the control group at 18 weeks.
Conclusion:3D printing navigation template can accurately locate the ONFH lesions. Implantation of BCP material can effectively play a supporting role, prevent the collapse of the loading surface, and induce bone formation and angiogenesis to some extent.
Preoperative fluorescent clip marking vs. India ink tattooing for tumor identification during colorectal surgery
Authors: Kitagawa Takahiro , Ryu Shunjin , Goto Keisuke , Okamoto Atsuko , Marukuchi Rui , Hara Keigo , Ito Ryusuke , Nakabayashi Yukio 
Published: July 2023

Abstract
Purpose
Identifying tumor location is important in colorectal tumor resection. Preoperative endoscopic India ink marking is a widespread practice, but local injection of ink is an unstable procedure. Although it is often invisible, the ink may be sprayed into the peritoneal cavity and contaminate the surgical field. At our hospital, we introduced fluorescent clip marking (FCM) using the Zeoclip FS®, an endoscopic clip developed using near-infrared fluorescent resin. We tested the usefulness of FCM by retrospectively comparing cases in which FCM was used with cases in which conventional ink marking was used.
Methods
We enrolled 305 patients with colorectal tumors who underwent colorectal surgery after preoperative marking from January 2017 to April 2022. We classified the patients into the FCM group (86 patients) and the India ink tattoo group (219 patients). Endoscopic marking was completed in the FCM group by the day before surgery, and fluorescence was evaluated during surgery with a fluorescent laparoscopic system. Patient backgrounds, marking visibility, adverse effects, and early postoperative results were retrospectively compared between groups.
Results
Marking was visually confirmed in 80 patients in the FCM group (93.02%) and in 166 patients in the India ink tattoo group (75.80%) (p = 0.0006). In the group with India ink tattoos, contamination of the surgical field was observed in seven cases (3.20%). No adverse events were observed in the FCM group.
Conclusion
In colorectal surgery, FCM provides better visibility than the conventional India ink tattooing method and is a simple and safe marking method.
Clinical trial registration
Examination of fluorescence navigation for laparoscopic colorectal cancer surgery.
Research Ethics Committee of the Kawaguchi Municipal Medical Center (Saitama, Japan) approval number: 2020-3. https://kawaguchi-mmc.org/wp-content/uploads/clinicalresearch-r02.pdf.
Determination of Ir(III) by Its Catalytic Effect on the Oxidation of Valine by HCF(III) in Aqueous Alkaline Medium- A Kinetic Spectrophotometric Method
Authors: Rajput Shakunj , Lasyal Rajni 
Published: July 2023

Abstract
A simple and sensitive spectrophotometric method has been developed for the determination of trace amounts of Ir(III). The method is based on the Ir(III) catalyzed oxidation of valine by HCF(III) in aqueous alkaline medium. Under the optimum experimental conditions, Beer’s law is obeyed in the range 1.99–19.98 µg ml− 1 of Ir(III) for valine with a maximum average error of 99.5% and maximum relative standard deviation of 1.702%. The value of the correlation coefficient lies between 0.999 to 0.980. The effect of few interfering ions has been worked out for developing the calibration curves in terms of absorbance or reaction rate or pseudo-first-order rate constant vs. [Ir (III)] plot, absorbance was noted at λmax of 420 nm. The molar absorptivity and Sandell’s sensitivity are found to be 0.9 x 103 to 1.2 x 103 lmol− 1cm− 1 and 0.216 to 0.213 µgcm− 2 respectively. The results show that the present method is more simple and more sensitive than the reported methods and especially useful in the absence of expected interfering ions. The standard deviations estimated from six independent determinations in a sample of Ir(III) are 0.088 to 0.11.
In Vitro Antiviral Activity of NanB Bacterial Sialidase Against Avian Influenza H9N2 Virus in MDCK cells
Authors: Nugroho Christian Marco Hadi , Silaen Otto Sahat Martua , Kurnia Ryan Septa , Krisnamurti Desak Gede Budi , Carnell George William , Putra Muhammad Ade , Indrawati Agustin , Poetri Okti Nadia , Wibawan I Wayan Teguh , Widyaningtyas Silvia Tri , Soebandrio Amin 
Published: July 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Study of fire behavior and evacuation in tunnel by simulation
Authors: Purwar Parth , Shukla Naman , Thanekar Arpit Thanekar , A.B Bharath , Bansod Shail , Bhattacharya Shantanu , Singh D.K. 
Published: July 2023

Abstract
Over the past few decades, fire protection in tunnels is becoming very important as tunnels are gaining more popularity. It is constructed and used for transportation by people and freight all over the world. In order to achieve a fire safety purpose, evacuation systems should be well designed and built to evacuate people as early and safely as possible. The objective of this paper is to investigate the fire behavior and evacuation in a tunnel based on numerical simulation models in a longitudinally ventilated tunnel. In this study, a single fire caused by vehicles carrying different fire loads is been considered and simulated along with multiple varying conditions, where installation of Heating Ventilation and Air Conditioning (HVAC’s) and its capability of reversing the spread of smoke i.e., to prevent back layering is been checked. FDS simulation modelling tool was also majorly used to investigate the dangerous situations in which a fully loaded gasoline tank lorry was considered for burning simulation inside a long road tunnel. These simulation results were used to determine conditions which are faced by humans while evacuating any road tunnel that’s under fire. In this paper, multiple model-based experiments were carried out using tunnels of different sizes and ventilation conditions.
DNA barcode-based survey documents underestimated diversity and intricate phylogeographic patterns of aquatic Heteroptera in an endangered Balkan biodiversity hotspot: ancient Lake Skadar basin
Authors: Rewicz Tomasz , Tończyk Grzegorz , Trębicki Łukasz , Gadawski Piotr , Mamos Tomasz , Pešić Vladimir , Zawal Andrzej , Grabowski Michał 
Published: July 2023

Abstract
Lake Skadar with its surrounding springs, wetlands and larger affluents is among the most diverse freshwater ecosystems in the Mediterranean region and a key biodiversity/endemism hotspot in Europe. It is also highly endangered due to climate change and rapid tourism development in the area. Being abundant, diverse and mostly predatory, true aquatic bugs play an important role in the functioning of freshwater ecosystems and are used as indicators of aquatic habitat quality. Nevertheless, this taxonomic group has been scarcely studied in the area. Our survey provides the first comprehensive DNA barcode library for 24 out of 25 species of aquatic Heteroptera collected in the Skadar Lake basin and adjacent regions. By this, we extend the list of species known from the area by 60%. In the case of three species, Notonecta maculata, Hydrometra stagnorum and Nepa cinerea, we detected multiple highly divergent, and also new BINs indicating possible taxonomic inconsistencies, the potential for (pseudo)cryptic diversity and intricate phylogeographic patterns. We show that presumably well-known hotspots, such as Lake Skadar region, are heavily understudied regarding even the prominent insect taxa and, thus, particularly vulnerable to undocumented biodiversity loss. Finally, we underline the value of simple DNA-barcoding-based surveys for providing reference barcode libraries for effective biomonitoring and signalling taxonomic and biogeographic issues.