bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative proteomics of vesicles essential for the egress of Plasmodium falciparum gametocytes from red blood cells
Authors: Sassmannshausen Juliane , Bennink Sandra , Distler Ute , Küchenhoff Juliane , Minns Allen M. , Lindner Scott E. , Burda Paul‐Christian , Tenzer Stefan , Gilberger Tim W. , Pradel Gabriele 
Published: July 2023

AbstractTransmission of malaria parasites to the mosquito is mediated by sexual precursor cells, the gametocytes. Upon entering the mosquito midgut, the gametocytes egress from the enveloping erythrocyte while passing through gametogenesis. Egress follows an inside‐out mode during which the membrane of the parasitophorous vacuole (PV) ruptures prior to the erythrocyte membrane. Membrane rupture requires exocytosis of specialized egress vesicles of the parasites; that is, osmiophilic bodies (OBs) involved in rupturing the PV membrane, and vesicles that harbor the perforin‐like protein PPLP2 (here termed P‐EVs) required for erythrocyte lysis. While some OB proteins have been identified, like G377 and MDV1/Peg3, the majority of egress vesicle‐resident proteins is yet unknown. Here, we used high‐resolution imaging and BioID methods to study the two egress vesicle types in Plasmodium falciparum gametocytes. We show that OB exocytosis precedes discharge of the P‐EVs and that exocytosis of the P‐EVs, but not of the OBs, is calcium sensitive. Both vesicle types exhibit distinct proteomes with the majority of proteins located in the OBs. In addition to known egress‐related proteins, we identified novel components of OBs and P‐EVs, including vesicle‐trafficking proteins. Our data provide insight into the immense molecular machinery required for the inside‐out egress of P. falciparum gametocytes.
State making or state breaking?’ Crisis, COVID‐19 and the constitution in Belgium, Spain and the United Kingdom
Authors: Anderson Paul , Swan Coree Brown , Ferreira Carles , Sijstermans Judith 
Published: July 2023

AbstractAs the first cases of COVID‐19 emerged in Belgium, Spain and the United Kingdom, they did so against a backdrop of heightened constitutional contestation. Capturing the period January 2020 to December 2021 which included three waves of the pandemic in each state and the delivery of vaccines, this article examines how state and sub‐state nationalists articulated their constitutional preferences and territorial claims in the pandemic period. We particularly explore whether the crisis changed state and sub‐state nationalists' territorial demands and how it was used to advance or bolster their territorial aspirations. We find that whilst the pandemic entailed an amplification of extant frames in favour of state dissolution and state integrity, the frames remained, broadly, similar between the pre‐pandemic and pandemic period, suggesting that sub‐state and state nationalist actors stick to a similar playbook, even at moments of profound crisis.
Plasticity in growth is genetically variable and highly conserved across spatial scales in a Mediterranean pine
Authors: de la Mata Raul , Zas Rafael 
Published: July 2023

SummaryPhenotypic plasticity is a main mechanism for sessile organisms to cope with changing environments. Plasticity is genetically based and can evolve under natural selection so that populations within a species show distinct phenotypic responses to environment. An important question that remains elusive is whether the intraspecific variation in plasticity at different spatial scales is independent from each other.To test whether variation in plasticity to macro‐ and micro‐environmental variation is related among each other, we used growth data of 25Pinus pinasterpopulations established in seven field common gardens in NW Spain. Phenotypic plasticity to macro‐environmental variation was estimated across test sites while plasticity to micro‐environmental variation was estimated by using semivariography and kriging for modeling within‐site heterogeneity.We provide empirical evidence of among‐population variation in the magnitude of plastic responses to both micro‐ and macro‐environmental variation. Importantly, we found that such responses were positively correlated across spatial scales.Selection for plasticity at one scale of environmental variation may impact the expression of plasticity at other scales, having important consequences on the ability of populations to buffer climate change. These results improve our understanding of the ecological drivers underlying the expression of phenotypic plasticity.
Testing the effects of banking development, economic growth and foreign direct investment on renewable energy in South Africa
Authors: Samour Ahmed , Ali Mumtaz , Abdullah Hariem , Moyo Delani , Tursoy Turgut 
Published: July 2023

AbstractThe world's climate‐related policies need to be revised to combat environmental issues. Therefore, adopting renewable energy technologies in conjunction with energy efficiency measures are needed to make safe pathways to reduce over 90% of energy related CO2. In this context, this study intends to assess the influence of foreign direct investment (FDI), economic growth, and banking and development on renewable energy consumption in South Africa from 1970 to 2020. The study uses a novel ‘bootstrap Autoregressive Distributed Lag (ARDL)’ testing to empirically analyse the short and long links among the tested variables. The ARDL estimations demonstrate a FDI and economic growth have positive effect on renewable energy consumption. Furthermore, the outcomes reveal that banking sector development affects positively renewable energy use in long run. Therefore, it is suggested that government and playmakers of South Africa must make more efforts to link the economic and baking development with renewable energy by investing capital and funds in renewable energy infrastructure. It will not only boost the economy of the country but also provide an opportunity to clean and green the environment with decreasing rate of greenhouse gases emission like carbon dioxide.
The evolutionary origin of the durophagous pelagic stingray ecomorph
Authors: Marramà Giuseppe , Villalobos‐Segura Eduardo , Zorzin Roberto , Kriwet Jürgen , Carnevale Giorgio 
Published: July 2023

AbstractStudies of the origin of evolutionary novelties (novel traits, feeding modes, behaviours, ecological niches, etc.) have considered a number of taxa experimenting with new body plans, allowing them to occupy new habitats and exploit new trophic resources. In the marine realm, colonization of pelagic environments by marine fishes occurred recurrently through time. Stingrays (Myliobatiformes) are a diverse clade of batoid fishes commonly known to possess venomous tail stings. Current hypotheses suggest that stingrays experimented with a transition from a benthic to a pelagic/benthopelagic habitat coupled with a transition from a non‐durophagous diet to extreme durophagy. However, there is no study detailing macroevolutionary patterns to understand how and when habitat shift and feeding specialization arose along their evolutionary history. A new exquisitely preserved fossil stingray from the Eocene Konservat‐Lagerstätte of Bolca (Italy) exhibits a unique mosaic of plesiomorphic features of the rajobenthic ecomorph, and derived traits of aquilopelagic taxa, that helps to clarify the evolutionary origin of durophagy and pelagic lifestyle in stingrays. A scenario of early evolution of the aquilopelagic ecomorph is proposed based on new data, and the possible adaptive meaning of the observed evolutionary changes is discussed. The body plan of †Dasyomyliobatis thomyorkei gen. et sp. nov. is intermediate between the rajobenthic and more derived aquilopelagic stingrays, supporting its stem phylogenetic position and the hypothesis that the aquilopelagic body plan arose in association with the evolution of durophagy and pelagic lifestyle from a benthic, soft‐prey feeder ancestor.
Differences in efficacy and safety between intrathecal infusion devices in cancer pain
Authors: Díaz‐Rodríguez Diego , Fontán‐Atalaya Isabel M. , Peralta‐Espinosa Estefanía , Fernández‐Torres Bartolomé 
Published: July 2023

AbstractBackgroundIntrathecal infusion therapy is widely accepted for cancer pain patients when conventional analgesic treatments are not sufficient. There are different types of devices for carrying out this therapy: partially externalized devices (PED), used when life expectancy is under 3 months, and totally implanted devices (TID), when it is larger. Our objective is to compare the efficacy, functionality, and complication rate in both types.MethodsWe included 132 patients with mild–severe cancer pain, treated with intrathecal infusion therapy by fixed flow devices, PED, or TID, during the study time. Demographic, physical oncologic, and pain control data of the patients were recorded prior to starting therapy and at months 1, 3, and 6. Functionality status and complications were also collected from the patient's medical records and clinical files.ResultsPain control improved after starting therapy, with an overall reduction of 4.75 points in VAS score at 1 month in the both groups, without significant differences between them, keeping it at 3 months and 6. 33.3% of the patients developed complications and were more frequent in the PED group, being catheter dislocation the most common. Patients with TID required more often hospital admission to solve the complication.ConclusionsIntrathecal infusion therapy has been shown to be a very effective and safe therapy for the treatment of moderate to severe oncologic pain. There are no significant differences between PED and TID in terms of degree of pain control, therapeutic success, efficacy on episodic or nocturnal pain, or the presence of serious complications.
Resistance that stacks up: engineering rust and mildew disease control in the cereal crops wheat and barley
Authors: Dracatos Peter M. , Lu Jing , Sánchez‐Martín Javier , Wulff Brande B.H. 
Published: July 2023

SummaryStaying ahead of the arms race against rust and mildew diseases in cereal crops is essential to maintain and preserve food security. The methodological challenges associated with conventional resistance breeding are major bottlenecks for deploying resistance (R) genes in high‐yielding crop varieties. Advancements in our knowledge of plant genomes, structural mechanisms, innovations in bioinformatics, and improved plant transformation techniques have alleviated this bottleneck by permitting rapid gene isolation, functional studies, directed engineering of synthetic resistance and precise genome manipulation in elite crop cultivars. Most cloned cereal R genes encode canonical immune receptors which, on their own, are prone to being overcome through selection for resistance‐evading pathogenic strains. However, the increasingly large repertoire of cloned R genes permits multi‐gene stacking that, in principle, should provide longer‐lasting resistance. This review discusses how these genomics‐enabled developments are leading to new breeding and biotechnological opportunities to achieve durable rust and powdery mildew control in cereals.
Policy coherence for the protection of water resources against agricultural pollution in the EU and Norway
Authors: Platjouw Froukje Maria , Nesheim Ingrid , Enge Caroline 
Published: July 2023

AbstractThroughout the European Union (EU), agricultural practices contribute significantly to the pollution of water resources by nitrates, phosphorus and pesticides. This article sheds light on the degree of horizontal legal coherence between the main EU legal and policy instruments applicable to the protection of water resources from agricultural pollution. After identifying key coherence challenges at the EU level, the article thoroughly assesses the regulatory and governance approach in Norway. The key question is how certain EU‐level coherence challenges could be mitigated at a national level through mechanisms aimed at facilitating cross‐sectoral coordination and policy coherence. Three types of mechanisms have been selected for this purpose: (i) legal mechanisms, including cross‐referencing and joint institutional responsibility for implementation; (ii) the establishment of platforms for cross‐sectoral policy coordination or actor participation; and (iii) the establishment of monitoring and reporting processes that ensure access to information and data sharing.
Accounting for Non‐Marketed capital
Authors: Cairns Robert D. , Davis Graham A. 
Published: July 2023

AbstractIntangible capital is a key input to production that is distinct from tangible capital. Most forms of tangible and intangible capital have observable, pecuniary values. Their complement is non‐marketed capital. This paper studies non‐marketed capital from the points of view of an investor contemplating investing in a project and of a manager running the project. At the point of investment, an apparent positive net present value is realized through the marshalling of non‐marketed capital. When capital is viewed all‐inclusively, a full accounting reveals that (1) optimally timing investment serves to maximize the value of the non‐marketed capital eventually associated with an investment project, (2) the “full” net present value at investment is zero, (3) the internal rate of return is identically equal to the external interest rate, (4) Tobin's q is identically equal to one, and (5) marginal analysis is inapplicable to non‐marketed capital and thus to all capital.
Barriers to routine dental care for children with special health care needs
Authors: Star Jean Marie , Flores Alain , Leyva Erick , Foertsch Christopher 
Published: July 2023

AbstractPurposeThe aim of this mixed method study was to identify barriers for children with special health care needs (SHCN) to receiving routine preventive dental care following restorative dental care with general anesthesia (GA).MethodsElectronic health records were reviewed for inclusion criteria and demographic data. Caregivers of children with SHCN were contacted to participate in qualitative interviews. Interview topics explored child, family, and community level influences to accessing routine dental care. Qualitative analysis identified key themes of barriers and enablers to care.ResultsA total of 1708 children received dental care with GA during the 2‐year study period, of which 498 (29.16%) had a diagnosis of a SHCN. The most common type of SHCN was neurodevelopmental disorders (28.51%). The mean age at time of GA was 8.6 years. Fifty caregivers completed interviews. Identified barriers to obtaining routine dental care included child stress/anxiety, finding an accepting provider, dismissive providers, and proximity of provider/transportation to dental care. Enablers to obtaining care included effective behavior management, continuity of provider/care, positive provider attitude, and referral to an accepting provider.ConclusionAdequately trained and local providers with an accepting attitude are essential to enabling children with SHCN to obtain equitable access to routine dental care.
Disordered eating in a 10‐year perspective from adolescence to young adulthood: Stability, change, and body dissatisfaction as a predictor
Authors: Foster Lo , Lundh Lars‐Gunnar , Daukantaité Daiva 
Published: July 2023

IntroductionAlthough there are many studies of disordered eating (DE) in adolescence, studies examining the longitudinal stability and change of DE and its longitudinal associations with other factors are still rather scarce. Such studies are important to inform parents and clinicians how stable DE is and to what extent it will go away with increased maturity. Longitudinal studies may also be of help in establishing predictors of long‐term problems. The purpose of the present study was to investigate the stability of DE over a 10‐year period from early adolescence to young adulthood, and to explore body dissatisfaction as a predictor in a 10‐year perspective.MethodsData were taken from a three‐wave longitudinal project (SoL) with survey data collected among the students in Grades 7and 8 in a Swedish municipality in 2007 (T1), 1 year later (T2), and in a 10‐year follow‐up in 2017 (T3). DE was measured by the eight‐item version of the Risk Behaviour Related to Eating Disorders (RiBED‐8), and a quasi‐clinical criterion of six or more critical answers on the RiBED‐8 was used to classify DE cases.ResultsOf the girls, 5.8% passed the cutoff for DE at T1, whereas 9.1% did so at T2 and 7.9% at T3. The boys showed markedly lower figures: 0.5% both at T1 and T2, and 1.5% at T3. There was significant individual stability of DE over the 10‐year period, with almost 30% of the girls who passed the cutoff for DE at T1 also doing so 10 years later. Body dissatisfaction in early adolescence predicted the incidence of new cases of DE 10 years later, even when controlling for degree of DE in early adolescence. The results also indicate that DE in early adolescence may be a passing phase among adolescents who express relatively high body satisfaction.ConclusionsThe present results show a complex picture of different individual trajectories of DE, with degree of body satisfaction playing a significant role for the outcome in a 10‐year perspective. Body dissatisfaction appears to be a risk factor for the development of new cases of DE, whereas body satisfaction may protect against the continuation of DE into adulthood.
Perceived COVID‐induced racism and contextual predictors of fear and psychological distress among Black men
Authors: Li K. Colin , Cipollina Rebecca , Sanchez Diana T. 
Published: July 2023

AbstractThe present study examined if Black men's perceptions of COVID‐induced racism (i.e., the extent to which racism increased during the beginning months of the COVID‐19 pandemic) were associated with their reported fear of COVID‐19 and psychological distress. Self‐report COVID‐related variables and psychological distress data from 231 Black men in the U.S. were analyzed alongside archival percentages of confirmed cases and deaths by participants' county at the time of participation. Hierarchical regressions identified perceptions of COVID‐induced racism as a significant predictor of Black men's psychological distress over and above contextual (e.g., county‐level COVID‐19 case %) and individual‐level predictors (i.e., perceived race‐related infection risk and self‐reported COVID‐related stressful life events). This work contributes to the growing literature on health inequities during the COVID‐19 pandemic with a snapshot of Black men's experiences during the COVID‐19 and racism pandemics of 2020 in the U.S.
Species of the cyprinid genusGarrain Mount Kenya, East Africa: Species delineation, taxonomy and historical biogeography
Authors: Cao Liang , Nyingi Wanja Dorothy , Bart Jr Henry L. , Zhang E 
Published: July 2023

AbstractThe cyprinid genusGarrais so far represented in Mount Kenya streams by a single species, but which species it should be referred to as remains yet to be determined. Molecular phylogenetic analysis ofGarraspecies from the mountain is still lacking. Here, an integrative analysis, based on morphological and molecular data, unravelled the hidden species diversity ofGarrafrom Mount Kenya. In mitochondrial genes‐based trees, samples formerly identified asGarra dembeensisfrom the mountain nested into three distinct lineages that were distantly allied to the lineage constituted by topotypical samples of the species; the three lineages are morphologically distinguishable, and thus represent three distinct species:Garra hindiiand two undescribed species, herein named asGarra alticaudasp. nov. andGarra minibarbatasp. nov., respectively, from the Mara River and the Ragati River of the mid‐upper Tana River basin. The phylogeographic pattern of the three species is incongruent with the present‐day basin pattern of Mount Kenya. The allopatry of the paired speciesG. alticaudaandG. hindiipoints to river piracy ever incurring between the upper Ewaso Ngiro River and the middle Tana River basin in Mount Kenya. Besides, the molecular phylogenetic analysis of sampled AfricanGarraspecies in this study, based on a broad set of sequences, provides evidence in support for the existing hypothesis of Asia‐to‐Africa biodispersal via the Arabian Peninsula to the Horn of Africa.
ADP-ribosylation-resistant rifabutin analogs show improved bactericidal activity against drug-tolerant
M. abscessus
in caseum surrogate
Authors: Xie Min , Ganapathy Uday S. , Lan Tian , Osiecki Paulina , Sarathy Jansy P. , Dartois Véronique , Aldrich Courtney C. , Dick Thomas 
Published: July 2023

ABSTRACT

Necrotic lesions and cavities filled with caseum are a hallmark of mycobacterial pulmonary disease. Bronchocavitary
Mycobacterium abscessus
disease is associated with poor treatment outcomes. In caseum surrogate,
M. abscessus
entered an extended stationary phase showing tolerance to killing by most current antibiotics, suggesting that caseum persisters contribute to the poor performance of available treatments. Novel ADP-ribosylation-resistant rifabutin analogs exhibited bactericidal activity against these
M. abscessus
persisters at concentrations achievable by rifamycins in caseum.
Feline herpesvirus 1 (FHV-1) enters the cell by receptor-mediated endocytosis
Authors: Synowiec Aleksandra , Dąbrowska Agnieszka , Pachota Magdalena , Baouche Meriem , Owczarek Katarzyna , Niżański Wojciech , Pyrc Krzysztof 
Published: July 2023

ABSTRACT

Feline herpesvirus type 1 (FHV-1) is an enveloped dsDNA virus belonging to the
Herpesviridae
family and is considered one of the two primary viral etiological factors of feline upper respiratory tract disease. In this study, we investigated the entry of FHV-1 into host cells using two models: the AK-D cell line and primary feline skin fibroblasts (FSFs). We employed confocal microscopy, siRNA silencing, and selective inhibitors of various entry pathways. Our observations revealed that the virus enters cells via pH and dynamin-dependent endocytosis, as the infection was significantly inhibited by NH
4
Cl, bafilomycin A1, dynasore, and mitmab. Additionally, genistein, nystatin, and filipin treatments, siRNA knock-down of caveolin-1, as well as FHV-1 and caveolin-1 colocalization suggest the involvement of caveolin-mediated endocytosis during the entry process. siRNA knock-down of clathrin heavy chain and analysis of virus particle colocalization with clathrin indicated that clathrin-mediated endocytosis also takes part in the primary cells. This is the first study to systematically examine FHV-1 entry into host cells, and for the first time, we describe FHV-1 replication in AK-D and FSFs.

IMPORTANCE
Feline herpesvirus 1 (FHV-1) is one of the most prevalent viruses in cats, causing feline viral rhinotracheitis, which is responsible for over half of viral upper respiratory diseases in cats and can lead to ocular lesions resulting in loss of sight. Although the available vaccine reduces the severity of the disease, it does not prevent infection or limit virus shedding. Despite the clinical relevance, the entry mechanisms of FHV-1 have not been thoroughly studied. Considering the limitations of commonly used models based on immortalized cells, we sought to verify our findings using primary feline skin fibroblasts, the natural target for infection in cats.