bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pembuatan Video Tutorial Make Up Pada Wajah Yang Memiliki Bekas Luka
Authors: Neneng Siti Silfi Ambarwati  , Fara Dilla Sari  
Published: February 2023

Abstrak
Pembuatan video tutorial make up pada wajah yang memiliki bekas luka hemangioma adalah video proses atau tahapan-tahapan dalam merias yang dirancang menjadi satu pada wajah yang memiliki bekas luka. Penelitian ini bertujuan untuk membuat sebuah produk berupa video tutorial yang layak. Proses di dalam pembuatan video ini terdiri dari proses perencanaan pra produksi, produksi, pasca produksi, proses penelitian, proses pengolahan data dan juga hasil penelitian.
Penelitian ini dilakukan pada seorang model yang memiliki bekas luka hemangioma pada wajah. Hasil pembuatan video ini divalidasi oleh dua ahli yaitu Ahli Media dan Ahli Materi. Memenuhi kriteria validasi dengan hasil yang didapat oleh ahli materi adalah 93,8%, dari ahli media 82,4%. Yang dapat disimpulkan bahwa video tersebut sangat layak untuk digunakan sebagai media alternatif serta mempermudah mahasiswa program studi tata rias dan masyarakat umum untuk mendapatkan informasi dan pengetahuan terkait video tutorial make up pada wajah yang memiliki bekas luka.

Kata kunci: Video Tutorial, Make Up, Wajah, Bekas Luka
Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena
Authors: Kikkawa Kyohei , Tanaka Minami , Kesumawati Elly , Koeda Sota 
Published: February 2023

Abstract
Yellow leaf curl disease caused by begomoviruses has emerged as a major limitation to the production of eggplant (Solanum melongena) in several regions of the world. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) is a bipartite begomovirus isolated from diseased eggplant in the areas of the Indochinese peninsula, South China, and Maritime Southeast Asia. In this study, five begomovirus species isolated in Indonesia, including TYLCKaV, were inoculated to eggplant using infectious clones. Among the inoculated begomoviruses, TYLCKaV alone induced typical yellowing and curling symptoms in eggplant. Inoculation of TYLCKaV to a panel of crop species (eggplant, tomato, pepper, tobacco, cucumber, melon, and squash) commonly grown in Indonesia clarified that TYLCKaV infects and causes yellow leaf curl disease mainly in eggplant and tomato. Agroinfiltration of TYLCKaV to 736 accessions of eggplant enabled selection of 33 resistant candidates. Further evaluation by TYLCKaV graft-inoculation of the resistant candidates clarified that 10 accessions were classifiable as moderately resistant and 23 accessions as highly resistant. Among the highly resistant accessions, 22 accessions restricted viral DNA accumulation to a significantly lower amount than the susceptible control. These 22 highly resistant accessions represent valuable genetic resources for breeding begomovirus resistance in eggplant.
Diagnostic Value of Neutrophil Cd64 in Sepsis Patients in the Intensive Care Unit: A Cross-sectional Study
Authors: Pham Huy Minh , Nguyen Duy Ly Minh , Duong Minh Cuong , Tran Linh Thanh , Pham Thao Thi Ngoc 
Published: February 2023

Abstract
Background
Sepsis is a major cause of morbidity and mortality in intensive care units (ICUs). The neutrophil CD64 (nCD64) index has been suggested to be a biomarker that can help early diagnose sepsis in these settings. However, little is known about the role of this biomarker in Asian patients. This study examined the cut-off and predictive values of nCD64 for diagnosing sepsis in Vietnamese ICU patients.
Methods
A cross-sectional study was conducted at the General ICU of Cho Ray Hospital between January 2019 and April 2020. All 104 newly admitted patients with or without sepsis were included. Sensitivity, specificity, positive predictive value, negative predictive value, and receiver operating characteristic (ROC) curves were calculated to compare the diagnostic value of nCD64 with that of serum procalcitonin (PCT) and while blood cell (WBC) count for sepsis.
Results
The median nCD64 index in sepsis patients was statistically higher than that of non-sepsis patients (3,106 [1,970-5,200] vs 745 [458-906] molecules/cell, P<0.001). ROC analysis found that the area under the ROC curve (AUC) value of nCD64 was 0.92, which was higher than that of PCT (0.872) and WBC (0.637), nCD64 combined with WBC (0.906), and nCD64 combined with WBC and PCT (0.919) but lower than that of nCD64 combined with PCT (0.924). With an AUC value of 0.92, the nCD64 index of 1,311 molecules/cell detected sepsis with a sensitivity of 89.9%, specificity of 85.7%, positive predictive value of 92.5%, and negative predictive value of 81.1%.
Conclusions
nCD64 can be used as a useful marker for early detecting sepsis in adult ICU patients. A combination of nCD64 and PCT may improve the diagnostic accuracy. More large studies are needed to examine the variance of the cut-off values of nCD64 for diagnosing sepsis in the wider Vietnamese population and comparable countries.
Representation of sensorimotor states in mouse medial prefrontal cortex
Authors: Kvitsiani Duda , Lopez-Yepez Junior , Barta Anna , Moltesen Maria , Martin Juliane , Woo Tsz-Fung , Hulme Oliver 
Published: February 2023

Abstract

When foraging for food, animals must track the sensory events of their environment and their own actions over time. Memory of these sensorimotor events is crucial for learning the values of different options and foraging policies1. To investigate the role of the medial prefrontal cortex (mPFC) in foraging behavior, we conducted experiments on mice using foraging tasks that required integration of past oro-sensory rewards and past choices. We found that the mPFC selectively represents sensorimotor events, which organize into a spatiotemporal map encoding location and temporal delay of past rewards and choices relative to the animal's current epoch in time. These representations of sensorimotor events, which we refer to as sensorimotor state representations, play a critical role in foraging behavior. Inactivation of the mPFC affected the integration of past rewards and choices into the mice's decisions, leading to a decrease in reward harvesting efficiency, particularly for longer temporal delays. Behavioral models that compute values and policy failed to capture the representations in mPFC. Our results suggest that the mPFC plays a critical role in representing sensorimotor states independently of value and policy computations. This highlights the importance of considering sensorimotor state representation in the mPFC in understanding foraging behavior.
Transcriptome Profiling Reveals the Genes Involved in Tuberous Root Expansion in Pueraria (Pueraria thomsonii Benth.)
Authors: Xufeng XIAO , Yuanfeng HU , ZHANG Ming , Shucheng SI , Haonan ZHOU , Weifeng ZHU , Fei GE , Caijun WU , Shuying FAN 
Published: February 2023

Abstract
Pueraria is a dry root commonly used in Traditional Chinese Medicine or as food and fodder, and tuberous root expansion is an important agronomic characteristic that influences its yield. However, current research has identified no specific genes regulating tuberous root expansion in Pueraria. Therefore, we aimed to explore the expansion mechanism of Pueraria at six developmental stages (P1–P6), by profiling the tuberous roots of an annual local variety “Gange No.1” harvested at 105, 135, 165, 195, 225, and 255 days after transplanting. Observations of the tuberous root phenotype and cell microstructural morphology revealed that the P3 stage was a critical boundary point in the expansion process, which was preceded by diameter thickening and yield gain rapidly of the tuberous roots, and followed by longitudinal elongation at both ends. A total of 17,441 differentially expressed genes (DEGs) were identified by comparing the P1 stage (unexpanded)against the P2–P6 stages (expanded) using transcriptome sequencing; 386 differential genes were shared across the six developmental stages. KEGG pathway enrichment analysis showed that the DEGs shared by P1 and P2–P6 stages were mainly involved in processes related to the “cell wall and cell cycle”, “plant hormone signal transduction”, and “sucrose and starch metabolism”. This finding is consistent with the physiological data collected on changes in sugar and hormone contents for Pueraria. The combination of KEGG and trend analyses revealed six essential candidate genes involved in tuberous root expansion; of them, CDC48, ARF, and EXP genes were significantly upregulated during tuberous root expansion while the INV, EXT, and XTH genes were significantly downregulated. These findings provide insights into the complex mechanisms of tuberous root expansion in Pueraria and candidate target genes, which can aid in increasing Pueraria yield.
Expression of genes related to iron homeostasis in breast cancer
Authors: Mutlu Tuba , Ozoran Emre , Trabulus Didem Can , Talu Canan Kelten , Erhan Duygu , Mete Meltem , Guven Mehmet 
Published: February 2023

Abstract
Background: The dysfunctions in the metabolism of iron has an important role in many pathological conditions, ranging from disease with iron deposition to cancer. Studies on malignant diseases of the breast reported irregular expression in genes associated with iron metabolism. The variations are related with findings which have prognostic significance. This study evaluated the relationship of the expression levels of transferrin receptor 1 (TFRC), iron regulatory protein 1 (IRP1), hepcidin (HAMP), ferroportin 1 (FPN1), hemojuvelin (HFE2), matriptase 2 (TMPRSS6), and miR-122 genes in the normal and malignant tissues of breast cancer patients.
Methods & Results: The normal and malignant tissues from 75 women with breast malignancies were used in this study. The patients did not receive any treatment previously. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used in figuring the levels of gene expression associated with the iron metabolism. When the malignant and normal tissues gene expression levels were analyzed, expression of TFRC increased (1.586-fold); IRP1 (0.594 fold) and miR-122 (0.320 fold) expression decreased; HAMP, FPN1, HFE2 and TMPRSS6 expressions did not change. FPN1 and IRP1 had a positive association, and this association was statistically significant (r=0.266; p=0.022). IRP1 and miR-122 had a positive association, and this association had statistical significance (r=0.231; p=0.048)
Conclusions: Our study portrayed the important association among genes involved in iron hemostasis and breast malignancy. The results could be used to establish new diagnostic techniques in the management of breast malignancies. The alterations in the metabolism of malignant breast cells with normal breast cells could be utilized to achieve advantages in treatment.
Mechanisms of Premotor-Motor Cortex Interactions during Goal Directed Behavior
Authors: Diester Ilka , Alyahyay Mansour , Kalweit Gabriel , Kalweit Maria , Karvat Golan , Ammer Julian , Schneider Artur , Adzemovic Ahmed , Vlachos Andreas , Bödecker Joschka 
Published: February 2023

Abstract
Deciphering the neural code underlying goal-directed behavior is a long-term mission in neuroscience1,2. Neurons exhibiting preparation and movement-related activity are intermingled in the premotor and motor cortices3,4, thus concealing the neural code of planned movements. We employed a combination of electrophysiology, pathway-specific optogenetics, phototagging, and inverse reinforcement learning (RL) to elucidate the role of defined neuronal subpopulations in the rat rostral and caudal forelimb areas (RFA and CFA), which correspond to the premotor and motor cortical areas. The inverse RL enabled the functional dissection of spatially intermingled neuronal subpopulations, complementing our pathway-specific optogenetic manipulations and unveiling differential functions of the preparation and movement subpopulations projecting from RFA to CFA. Our results show that the projecting preparation subpopulation suppresses movements, whereas the projecting movement subpopulation promotes actions. We found the influence of RFA on CFA to be adaptable, with the projection either inhibiting or exciting neurons in the superficial and deep CFA layers, depending on context and task phase. These complex interactions between RFA and CFA likely involve the differential recruitment of inhibitory interneurons in the CFA, which is supported by our electron microscopy analysis of the connectivity between these regions. We provide here unprecedented mechanistic insights into how the premotor and primary motor cortices are functionally and structurally interlinked with the potential to advance neuroprosthetics.
Human Cancer Risk due to Chromium and its Bioaccumulation in Physids in Central Punjab, Pakistan
Authors: Aziz Safia - , Altaf Javaria , Khalil Abdullah - , Khalil Hassan , Jehangir Kiran - , Awan Faisal Saeed 
Published: February 2023

Abstract
The present study aims to assess the ecological risk of chromium in the water and soil of Central Punjab and its natural bioremediation through Physids. Genus Physa has been found cosmopolitan and resistant to pollutants. The snails of the genus Physa were sampled from October through March and three species were identified. The foot, shell, water, and soil samples were tested for the presence of the heavy metal chromium, using ICP-MS. The results show the concentration of chromium exceeds the National Environmental Quality Standards in water making it extremely high cancer risk for human consumption and various areas of Punjab (Pakistan) have been found extremely polluted due to chromium. No significant difference among the three species was found in terms of bioaccumulation of chromium in their snail shell and body. Physids play an active role in the bioremediation of soil and water yet may become cancer-causing tablets in the food chains of the region.
A biomolecular magnetic switch by diamagnetic cations
Authors: Yang Haijun , Mu Liuhua , Zhang Lei , Wang Zixin , Sheng Shiqi , Wang Jihong , Chen Hanxiao , Song Yongshun , Jiang Jie , Xiu Peng , Tong Wei , Zhang Yueyu , Song Sanzhao , Zhang Nian , Cao Jiefeng , Zhu Fangyuan , Zhou Limin , Zhang Lijuan , Shi Guosheng , Hu Jun , Wang Yong , Zhang Xin , Zhang Feng , Fang Haiping 
Published: February 2023

Abstract
The magnetism of proteins and their contributions to magnetoreception and other magnetic bio-effects have been debated for decades, mostly focusing on the insufficient iron atoms and lacking physical explanations. Here we show that divalent metal cations alone, including Mg, Ca, and Zn cations, which are not traditional magnetic materials, can abruptly switch aromatic biomolecules from diamagnetic to exceptionally strongly paramagnetic. Remarkably, their paramagnetism is so strong, together with sequence-dependence, enabling the separation of DNA with different sequences under a moderate magnetic field, thus resulting in a DNA magnetophoresis prototype without any magnetic particle addition. Theoretical computations show that water helps stabilize the hydrated MgCl on the single aromatic ring via hydrated cation–π interactions, and the complex of the hydrated MgCl on the single aromatic ring possesses a magnetic moment of 1.0 μB, which induces the exceptionally strong paramagnetism of the biomolecules with cations. X-ray experiments demonstrate the novel electronic states of the cations adsorbed on aromatic rings, indicating the existence of magnetic moments in the complex. The finding reveals a new origin for the exceptionally strong paramagnetism and biological magnetism, provides new insights into the interactions between biological systems and magnetic fields, and will greatly benefit magnetically controlled applications and beyond.
Stroke as Initial Manifestation of Non-Small Cell Lung Cancer with Trousseau Syndrome
Authors: Hong Weiping , Zhang Tongtong , Shan Changguo , Li Hainan , Lin Tao , Liu Da , Zhen Junjie , Li Juan , Lai Mingyao , Zhou Zhaoming , Zhou Cheng , Zhou Meijuan , Wang Minghua , Cai Linbo , Wen Lei 
Published: February 2023

Abstract
Objective
Stroke is a rare but fatal complication of advanced cancer with Trousseau syndrome, especially as initial symptoms. Here, we report the clinical characteristics, treatment, and prognosis of patients with non-small cell lung cancer (NSCLC) who initially presenting with acute multiple cerebral infarction.
Methods
The clinical characteristics, imaging, treatment, and oncological outcomes of 10 patients diagnosed with Trousseau syndrome and NSCLC between 2015 and 2021 at Guangdong Sanjiu Brain Hospital were retrospectively collected and analyzed. The clinical course of two typical cases were presented.
Results
All 10 patients with pathologically confirmed lung adenocarcinoma initially presented with neurological symptoms, including hemiplegic paralysis (7 patients, 70%), dizziness (5 patients, 50%), and unclear speech (3 patients, 30%). The median age was 63.5 years. Eight and two cases were stage III and IV, respectively, at the initial diagnosis. Five patients underwent driver gene testing, revealing three patients with EGFR-sensitive mutations, one patient with ALK fusion, and one patient with wild-type EGFR. All 10 patients received antiplatelet therapy, and six patients subsequently received anti-cancer treatment. The median overall survival of the patients was 8.5 months (95% confidence interval) and 1-year survival rate was 57.1%. Patients who received antitumor treatment, especially those harboring driver gene mutations and received tyrosine kinase inhibitors, had better neurological symptom recovery and superior oncological prognosis (median overall survival, not reached versus 7.4 months, p = 0.038).
Conclusion
Trousseau syndrome, presenting as multiple cerebral infarctions, is a rare complication of lung adenocarcinoma. Both antiplatelet and antitumor treatment are recommended to achieve better neurological recovery and oncological prognosis in these patients.
Low back pain (LBP) prevalence and its role in depressive symptoms among nurses in Damascus, Syria – a cross-sectional study
Authors: Aldroubi Louna , Safiah Mhd Homam , Ashabi Khaled Kalalib AL , Khalayli Naram , Kudsi Maysoun 
Published: February 2023

Abstract
Background: Nurses are more predisposed to low back pain (LBP), due to the nature of their work. Its prevalence varies from 62% to 84%. We aimed to reveal low back pain prevalence, and its characteristics, including its relation with depressive symptoms among a sample of Syrian nurses.
Methods: A cross-sectional study was conducted on 156 female nurses, working at Al Mouwasat hospital, Syria, between July 2021 to July 2022. Demographic data, occupational factors data, and psychological data including the presence of symptoms of depression were collected. The use scales were: the Numerical Rating one to measure pain and the Center for Epidemiologic Studies Depression Scale to measure depression.
Results: Out of 156 evaluated nurses, 90 (57.7%) of them have had lower back pain within the last year. 60% suffered from acute mechanical pain. The presence of Low back pain was correlated with physical activity, duration of work (in years), and type of work that include lifting. The prevalence of depressive symptoms was found in 46 (29.5%) nurses. Low back pain was found in 33 (71.7%) of the nurses who had depressive symptoms with a static significance deference as nurses with low back pain are more likely to have depressive symptoms and other factors are also correlated such as the duration of pain.
Conclusion: The nursing staff is one of the crucial cornerstones of the Syrian healthcare system, which has been struggling for years. Both low back pain and depressive symptoms were found to be highly prevalent. In nurses who had depressive symptoms, the majority of low back pain was greater, and this difference was statistically significant. This study's findings must be actively addressed, and precautions must be taken.
Systematic Reduction of Gray Matter Volume in Anorexia Nervosa, but Increase With Clinical Symptoms in the Prefrontal and Posterior Insular Cortices: A Multicenter Neuroimaging Study
Authors: Sekiguchi Atsushi , TOSE Keima , Takamura Tsunehiko , Isobe Masanori , Hirano Yoshiyuki , SATO Yasuhiro , Kodama Naoki , YOSHIHARA Kazufumi , MAIKUSA Norihide , Moriguchi Yoshiya , NODA Tomomi , Mishima Ryo , KAWABATA Michiko , NOMA Shun'ichi , TAKAKURA Shu , GONDO Motoharu , Kakeda Shingo , Takahashi Masatoshi , Ide Satoru , Adachi Hiroaki , HAMATANI Sayo , KAMASHITA Rio , MATSUMOTO Koji , NAKAZATO Michiko , NUMATA Noriko , HAMAMOTO Yumi , SHOJI Tomotaka , MURATSUBAKI Tomohiko , SUGIURA Motoaki , Murai Toshiya , Fukudo Shin 
Published: February 2023

Abstract
Although brain morphological abnormalities have been reported in anorexia nervosa (AN), the reliability and reproducibility of previous studies were limited due to insufficient sample sizes, which prevented exploratory analysis of the whole brain as opposed to regions of interest (ROIs). Objective was to identify brain morphological abnormalities in AN and the association with severity of AN by brain structural magnetic resonance imaging (MRI) in a multicenter study, and to conduct exploratory analysis of the whole brain. Here, ｗe conducted a cross-sectional multicenter study using T1-weighted imaging (T1WI) data collected between May 2014 and February 2019 in Japan. We analyzed MRI data from 103 female AN patients (58 anorexia nervosa restricting type [ANR] and 45 anorexia nervosa binge-purging type [ANBP]) and 102 age-matched female healthy controls (HCs). MRI data from five centers were preprocessed using the latest harmonization method to correct for intercenter differences. Gray matter volume (GMV) was calculated from T1WI data of all participants. Of the 205 participants, we obtained severity of eating disorder symptom scores from 179 participants, including 87 in the AN group (51 ANR, 36 AMBP) and 92 HCs using the Eating Disorder Examination Questionnaire (EDE-Q) 6.0. GMV reduction were observed in the AN brain, including the bilateral cerebellum, middle and posterior cingulate gyrus, supplementary motor cortex, precentral gyrus medial segment, and thalamus. In addition, the orbitofrontal cortex (OFC), ventromedial prefrontal cortex (vmPFC), rostral anterior cingulate cortex (ACC), and posterior insula volumes showed positive correlations with severity of symptoms. This multicenter study was conducted with a large sample size to identify brain morphological abnormalities in AN. The findings provide a better understanding of the pathogenesis of AN and have potential for the development of brain imaging biomarkers of AN.
Trial Registration: University Hospital Medical Information Network Individual Case Data Repository: UMIN000017456. https://center6.umin.ac.jp/cgi-open-bin/icdr/ctr_view.cgi?recptno=R000019303