bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paleogeographic Reconstruction of Precambrian Terranes Reworked by Phanerozoic Orogens: An Example Based on Detrital Zircon REE From Lhasa Terrane in Southern Tibet
Authors: Hu Pei‐yuan , Zhai Qing‐guo , Cawood Peter A. , Weinberg Roberto F. , Zhao Guo‐chun , Tang Yue , Liu Yi‐ming 
Published: March 2023

AbstractPaleogeographic reconstruction of Precambrian terranes reworked by Phanerozoic orogens (e.g., the Tibetan Plateau) results in complex lithotectonic relations due to intracrustal reworking by tectonothermal events. Detrital zircon rare earth element (REE) databases at global (global major river sands) and regional (the Gangdese Mountains, southern Tibet) scales reveal trends in and Eu/Eu* that effectively record the crustal evolution of the source, including crustal thickness and redox state of the magma that generated the zircons. Regional comparisons of these chemical markers provide a new approach for paleogeographic reconstructions that we apply to study the origin of the Lhasa terrane, southern Tibet. Using Precambrian to early Paleozoic sedimentary and igneous rocks in the Lhasa terrane and compiling detrital zircon analyses from the northern margin of Gondwana, we show that the Lhasa terrane had an African affinity in the Rodinia–Gondwana supercontinent cycles (ca. 1.4–0.4 Ga).
Arbitrarily polarized bound states in the continuum with twisted photonic crystal slabs
Authors: Qin Haoye , Su Zengping , Liu Mengqi , Zeng Yixuan , Tang Man-Chung , Li Mengyao , Shi Yuzhi , Huang Wei , Qiu Cheng-Wei , Song Qinghua 
Published: March 2023

AbstractArbitrary polarized vortex beam induced by polarization singularity offers a new platform for both classical optics and quantum entanglement applications. Bound states in the continuum (BICs) have been demonstrated to be associated with topological charge and vortex polarization singularities in momentum space. For conventional symmetric photonic crystal slabs (PhCSs), BIC is enclosed by linearly polarized far fields with winding angle of 2π, which is unfavorable for high-capacity and multi-functionality integration-optics applications. Here, we show that by breaking σz-symmetry of the PhCS, asymmetry in upward and downward directions and arbitrarily polarized BIC can be realized with a bilayer-twisted PhCS. It exhibits elliptical polarization states with constant ellipticity angle at every point in momentum space within the vicinity of BIC. The topological nature of BIC reflects on the orientation angle of polarization state, with a topological charge of 1 for any value of ellipticity angle. Full coverage of Poincaré sphere (i.e., $$- {{\pi \over 4}} \le \chi \le {{\pi \over 4}}$$

−

π

4

≤
χ
≤

π

4

and $$- {{\pi \over 2}} \le \psi \le {{\pi \over 2}}$$

−

π

2

≤
ψ
≤

π

2

) and higher-order Poincaré sphere can be realized by tailoring the twist angles. Our findings may open up new avenues for applications in structured light, quantum optics, and twistronics for photons.
Deep clinical phenotyping of patients with obsessive-compulsive disorder: an approach towards detection of organic causes and first results
Authors: Runge Kimon , Reisert Marco , Feige Bernd , Nickel Kathrin , Urbach Horst , Venhoff Nils , Tzschach Andreas , Schiele Miriam A. , Hannibal Luciana , Prüss Harald , Domschke Katharina , Tebartz van Elst Ludger , Endres Dominique 
Published: March 2023

AbstractIn the revised diagnostic classification systems ICD-11 and DSM-5, secondary, organic forms of obsessive-compulsive disorder (OCD) are implemented as specific nosological entities. Therefore, the aim of this study was to clarify whether a comprehensive screening approach, such as the Freiburg-Diagnostic-Protocol for patients with OCD (FDP-OCD), is beneficial for detecting organic OCD forms. The FDP-OCD includes advanced laboratory tests, an expanded magnetic resonance imaging (MRI) protocol, and electroencephalography (EEG) investigations as well as automated MRI and EEG analyses. Cerebrospinal fluid (CSF), [18F]fluorodeoxyglucose positron emission tomography, and genetic analysis were added for patients with suspected organic OCD. The diagnostic findings of the first 61 consecutive OCD inpatients (32 female and 29 male; mean age: 32.7 ± 12.05 years) analyzed using our protocol were investigated. A probable organic cause was assumed in five patients (8%), which included three patients with autoimmune OCD (one patient with neurolupus and two with specific novel neuronal antibodies in CSF) and two patients with newly diagnosed genetic syndromes (both with matching MRI alterations). In another five patients (8%), possible organic OCD was detected (three autoimmune cases and two genetic cases). Immunological serum abnormalities were identified in the entire patient group, particularly with high rates of decreased “neurovitamin” levels (suboptimal vitamin D in 75% and folic acid in 21%) and increased streptococcal (in 46%) and antinuclear antibodies (ANAs; in 36%). In summary, the FDP-OCD screening led to the detection of probable or possible organic OCD forms in 16% of the patients with mostly autoimmune forms of OCD. The frequent presence of systemic autoantibodies such as ANAs further support the possible influence of autoimmune processes in subgroups of patients with OCD. Further research is needed to identify the prevalence of organic OCD forms and its treatment options.
Early-life stress biases responding to negative feedback and increases amygdala volume and vulnerability to later-life stress
Authors: Dutcher Ethan G. , Lopez-Cruz Laura , Pama E. A. Claudia , Lynall Mary-Ellen , Bevers Iris C. R. , Jones Jolyon A. , Khan Shahid , Sawiak Stephen J. , Milton Amy L. , Clatworthy Menna R. , Robbins Trevor W. , Bullmore Edward T. , Dalley Jeffrey W. 
Published: March 2023

AbstractEarly-life stress (ELS) or adversity, particularly in the form of childhood neglect and abuse, is associated with poor mental and physical health outcomes in adulthood. However, whether these relationships are mediated by the consequences of ELS itself or by other exposures that frequently co-occur with ELS is unclear. To address this question, we carried out a longitudinal study in rats to isolate the effects of ELS on regional brain volumes and behavioral phenotypes relevant to anxiety and depression. We used the repeated maternal separation (RMS) model of chronic ELS, and conducted behavioral measurements throughout adulthood, including of probabilistic reversal learning (PRL), responding on a progressive ratio task, sucrose preference, novelty preference, novelty reactivity, and putative anxiety-like behavior on the elevated plus maze. Our behavioral assessment was combined with magnetic resonance imaging (MRI) for quantitation of regional brain volumes at three time points: immediately following RMS, young adulthood without further stress, and late adulthood with further stress. We found that RMS caused long-lasting, sexually dimorphic biased responding to negative feedback on the PRL task. RMS also slowed response time on the PRL task, but without this directly impacting task performance. RMS animals were also uniquely sensitive to a second stressor, which disproportionately impaired their performance and slowed their responding on the PRL task. MRI at the time of the adult stress revealed a larger amygdala volume in RMS animals compared with controls. These behavioral and neurobiological effects persisted well into adulthood despite a lack of effects on conventional tests of ‘depression-like’ and ‘anxiety-like’ behavior, and a lack of any evidence of anhedonia. Our findings indicate that ELS has long-lasting cognitive and neurobehavioral effects that interact with stress in adulthood and may have relevance for understanding the etiology of anxiety and depression in humans.
Reprogramming of palmitic acid induced by dephosphorylation of ACOX1 promotes β-catenin palmitoylation to drive colorectal cancer progression
Authors: Zhang Qiang , Yang Xiaoya , Wu Jinjie , Ye Shubiao , Gong Junli , Cheng Wai Ming , Luo Zhanhao , Yu Jing , Liu Yugeng , Zeng Wanyi , Liu Chen , Xiong Zhizhong , Chen Yuan , He Zhen , Lan Ping 
Published: March 2023

AbstractMetabolic reprogramming is a hallmark of cancer. However, it is not well known how metabolism affects cancer progression. We identified that metabolic enzyme acyl-CoA oxidase 1 (ACOX1) suppresses colorectal cancer (CRC) progression by regulating palmitic acid (PA) reprogramming. ACOX1 is highly downregulated in CRC, which predicts poor clinical outcome in CRC patients. Functionally, ACOX1 depletion promotes CRC cell proliferation in vitro and colorectal tumorigenesis in mouse models, whereas ACOX1 overexpression inhibits patient-derived xenograft growth. Mechanistically, DUSP14 dephosphorylates ACOX1 at serine 26, promoting its polyubiquitination and proteasomal degradation, thereby leading to an increase of the ACOX1 substrate PA. Accumulated PA promotes β-catenin cysteine 466 palmitoylation, which inhibits CK1- and GSK3-directed phosphorylation of β-catenin and subsequent β-Trcp-mediated proteasomal degradation. In return, stabilized β-catenin directly represses ACOX1 transcription and indirectly activates DUSP14 transcription by upregulating c-Myc, a typical target of β-catenin. Finally, we confirmed that the DUSP14-ACOX1-PA-β-catenin axis is dysregulated in clinical CRC samples. Together, these results identify ACOX1 as a tumor suppressor, the downregulation of which increases PA-mediated β-catenin palmitoylation and stabilization and hyperactivates β-catenin signaling thus promoting CRC progression. Particularly, targeting β-catenin palmitoylation by 2-bromopalmitate (2-BP) can efficiently inhibit β-catenin-dependent tumor growth in vivo, and pharmacological inhibition of DUSP14-ACOX1-β-catenin axis by Nu-7441 reduced the viability of CRC cells. Our results reveal an unexpected role of PA reprogramming induced by dephosphorylation of ACOX1 in activating β-catenin signaling and promoting cancer progression, and propose the inhibition of the dephosphorylation of ACOX1 by DUSP14 or β-catenin palmitoylation as a viable option for CRC treatment.
Self-folding soft-robotic chains with reconfigurable shapes and functionalities
Authors: Gu Hongri , Möckli Marino , Ehmke Claas , Kim Minsoo , Wieland Matthias , Moser Simon , Bechinger Clemens , Boehler Quentin , Nelson Bradley J. 
Published: March 2023

AbstractMagnetic continuum soft robots can actively steer their tip under an external magnetic field, enabling them to effectively navigate in complex in vivo environments and perform minimally invasive interventions. However, the geometries and functionalities of these robotic tools are limited by the inner diameter of the supporting catheter as well as the natural orifices and access ports of the human body. Here, we present a class of magnetic soft-robotic chains (MaSoChains) that can self-fold into large assemblies with stable configurations using a combination of elastic and magnetic energies. By pushing and pulling the MaSoChain relative to its catheter sheath, repeated assembly and disassembly with programmable shapes and functions are achieved. MaSoChains are compatible with state-of-the-art magnetic navigation technologies and provide many desirable features and functions that are difficult to realize through existing surgical tools. This strategy can be further customized and implemented for a wide spectrum of tools for minimally invasive interventions.
Limitations of gene editing assessments in human preimplantation embryos
Authors: Liang Dan , Mikhalchenko Aleksei , Ma Hong , Marti Gutierrez Nuria , Chen Tailai , Lee Yeonmi , Park Sang-Wook , Tippner-Hedges Rebecca , Koski Amy , Darby Hayley , Li Ying , Van Dyken Crystal , Zhao Han , Wu Keliang , Zhang Jingye , Hou Zhenzhen , So Seongjun , Han Jongsuk , Park Jumi , Kim Chong-Jai , Zong Kai , Gong Jianhui , Yuan Yilin , Gu Ying , Shen Yue , Olson Susan B. , Yang Hui , Battaglia David , O’Leary Thomas , Krieg Sacha A. , Lee David M. , Wu Diana H. , Duell P. Barton , Kaul Sanjiv , Kim Jin-Soo , Heitner Stephen B. , Kang Eunju , Chen Zi-Jiang , Amato Paula , Mitalipov Shoukhrat 
Published: March 2023

AbstractRange of DNA repair in response to double-strand breaks induced in human preimplantation embryos remains uncertain due to the complexity of analyzing single- or few-cell samples. Sequencing of such minute DNA input requires a whole genome amplification that can introduce artifacts, including coverage nonuniformity, amplification biases, and allelic dropouts at the target site. We show here that, on average, 26.6% of preexisting heterozygous loci in control single blastomere samples appear as homozygous after whole genome amplification indicative of allelic dropouts. To overcome these limitations, we validate on-target modifications seen in gene edited human embryos in embryonic stem cells. We show that, in addition to frequent indel mutations, biallelic double-strand breaks can also produce large deletions at the target site. Moreover, some embryonic stem cells show copy-neutral loss of heterozygosity at the cleavage site which is likely caused by interallelic gene conversion. However, the frequency of loss of heterozygosity in embryonic stem cells is lower than in blastomeres, suggesting that allelic dropouts is a common whole genome amplification outcome limiting genotyping accuracy in human preimplantation embryos.
Direct intranodal tonsil vaccination with modified vaccinia Ankara vaccine protects macaques from highly pathogenic SIVmac251
Authors: Mattathil Jeffy G. , Volz Asisa , Onabajo Olusegun O. , Maynard Sean , Bixler Sandra L. , Shen Xiaoying X. , Vargas-Inchaustegui Diego , Robert-Guroff Marjorie , Lebranche Celia , Tomaras Georgia , Montefiori David , Sutter Gerd , Mattapallil Joseph J. 
Published: March 2023

AbstractHuman immunodeficiency virus (HIV) is a mucosally transmitted virus that causes immunodeficiency and AIDS. Developing efficacious vaccines to prevent infection is essential to control the epidemic. Protecting the vaginal and rectal mucosa, the primary routes of HIV entry has been a challenge given the significant compartmentalization between the mucosal and peripheral immune systems. We hypothesized that direct intranodal vaccination of mucosa associated lymphoid tissue (MALT) such as the readily accessible palatine tonsils could overcome this compartmentalization. Here we show that rhesus macaques primed with plasmid DNA encoding SIVmac251-env and gag genes followed by an intranodal tonsil MALT boost with MVA encoding the same genes protects from a repeated low dose intrarectal challenge with highly pathogenic SIVmac251; 43% (3/7) of vaccinated macaques remained uninfected after 9 challenges as compared to the unvaccinated control (0/6) animals. One vaccinated animal remained free of infection even after 22 challenges. Vaccination was associated with a ~2 log decrease in acute viremia that inversely correlated with anamnestic immune responses. Our results suggest that a combination of systemic and intranodal tonsil MALT vaccination could induce robust adaptive and innate immune responses leading to protection from mucosal infection with highly pathogenic HIV and rapidly control viral breakthroughs.
Transient dynamics of the phase transition in VO2 revealed by mega-electron-volt ultrafast electron diffraction
Authors: Xu Chenhang , Jin Cheng , Chen Zijing , Lu Qi , Cheng Yun , Zhang Bo , Qi Fengfeng , Chen Jiajun , Yin Xunqing , Wang Guohua , Xiang Dao , Qian Dong 
Published: March 2023

AbstractVanadium dioxide (VO2) exhibits an insulator-to-metal transition accompanied by a structural transition near room temperature. This transition can be triggered by an ultrafast laser pulse. Exotic transient states, such as a metallic state without structural transition, were also proposed. These unique characteristics let VO2 have great potential in thermal switchable devices and photonic applications. Although great efforts have been made, the atomic pathway during the photoinduced phase transition is still not clear. Here, we synthesize freestanding quasi-single-crystal VO2 films and examine their photoinduced structural phase transition with mega-electron-volt ultrafast electron diffraction. Leveraging the high signal-to-noise ratio and high temporal resolution, we observe that the disappearance of vanadium dimers and zigzag chains does not coincide with the transformation of crystal symmetry. After photoexcitation, the initial structure is strongly modified within 200 femtoseconds, resulting in a transient monoclinic structure without vanadium dimers and zigzag chains. Then, it continues to evolve to the final tetragonal structure in approximately 5 picoseconds. In addition, only one laser fluence threshold instead of two thresholds suggested in polycrystalline samples is observed in our quasi-single-crystal samples. Our findings provide essential information for a comprehensive understanding of the photoinduced ultrafast phase transition in VO2.
A Global Database of Soil Plant Available Phosphorus
Authors: McDowell R. W. , Noble A. , Pletnyakov P. , Haygarth P. M. 
Published: March 2023

AbstractSoil phosphorus drives food production that is needed to feed a growing global population. However, knowledge of plant available phosphorus stocks at a global scale is poor but needed to better match phosphorus fertiliser supply to crop demand. We collated, checked, converted, and filtered a database of c. 575,000 soil samples to c. 33,000 soil samples of soil Olsen phosphorus concentrations. These data represent the most up-to-date repository of freely available data for plant available phosphorus at a global scale. We used these data to derive a model (R2 = 0.54) of topsoil Olsen phosphorus concentrations that when combined with data on bulk density predicted the distribution and global stock of soil Olsen phosphorus. We expect that these data can be used to not only show where plant available P should be boosted, but also where it can be drawn down to make more efficient use of fertiliser phosphorus and to minimise likely phosphorus loss and degradation of water quality.
WAVES – The Lucile Packard Children’s Hospital Pediatric Physiological Waveforms Dataset
Authors: Miller Daniel R. , Dhillon Gurpreet S. , Bambos Nicholas , Shin Andrew Y. , Scheinker David 
Published: March 2023

AbstractWAVES is a large, single-center dataset comprising 9 years of high-frequency physiological waveform data from patients in intensive and acute care units at a large academic, pediatric medical center. The data comprise approximately 10.6 million hours of 1 to 20 concurrent waveforms over approximately 50,364 distinct patient encounters. The data have been de-identified, cleaned, and organized to facilitate research. Initial analyses demonstrate the potential of the data for clinical applications such as non-invasive blood pressure monitoring and methodological applications such as waveform-agnostic data imputation. WAVES is the largest pediatric-focused and second largest physiological waveform dataset available for research.
CD24-associated ceRNA network reveals prognostic biomarkers in breast carcinoma
Authors: Yu Bin , Zhao Ziyue , Chen Zhuoyuan , Xiang Cheng , Wang Pingxiao , Xiao Bo , Xia Yu , Li Aoyu , Xiao Tao , Li Hui 
Published: March 2023

AbstractBreast cancer is one of the most common cancer types which is described as the leading cause of cancer death in women. After competitive endogenous RNA (ceRNA) hypothesis was proposed, this triple regulatory network has been observed in various cancers, and increasing evidences reveal that ceRNA network plays a significant role in the migration, invasion, proliferation of cancer cells. In the current study, our target is to construct a CD24-associated ceRNA network, and to further identify key prognostic biomarkers in breast cancer. Using the transcriptom profiles from TCGA database, we performed a comprehensive analysis between CD24high tumor samples and CD24low tumor samples, and identified 132 DElncRNAs, 602 DEmRNAs and 26 DEmiRNAs. Through comprehensive analysis, RP1-228H13.5/miR-135a-5p/BEND3 and SIM2 were identified as key CD24-associated biomarkers, which exhibited highly significance with overall survival, immune microenvironment as well as clinical features. To sum up the above, the current study constructed a CD24-associated ceRNA network, and RP1-228H13.5/miR-135a-5p/BEND3 and SIM2 axis worked as a potential therapeutic target and a predictor for BRCA diagnosis and prognosis.
Defining metabolic migraine with a distinct subgroup of patients with suboptimal inflammatory and metabolic markers
Authors: Gross Elena C. , Putananickal Niveditha , Orsini Anna-Lena , Schoenen Jean , Fischer Dirk , Soto-Mota Adrian 
Published: March 2023

AbstractEmerging evidence suggest migraine is a response to cerebral energy deficiency or oxidative stress in the brain. Beta-hydroxybutyrate (BHB) is likely able to circumvent some of the meta-bolic abnormalities reported in migraine. Exogenous BHB was given to test this assumption and, in this post-hoc analysis, multiple metabolic biomarkers were identified to predict clinical improvements. A randomized clinical trial, involving 41 patients with episodic migraine. Each treatment period was 12 weeks long, followed by eight weeks of washout phase / second run-in phase before entering the corresponding second treatment period. The primary endpoint was the number of migraine days in the last 4 weeks of treatment adjusted for baseline. BHB re-sponders were identified (those with at least a 3-day reduction in migraine days over placebo) and its predictors were evaluated using Akaike’s Information Criterion (AIC) stepwise boot-strapped analysis and logistic regression. Responder analysis showed that metabolic markers could identify a “metabolic migraine” subgroup, which responded to BHB with a 5.7 migraine days reduction compared to the placebo. This analysis provides further support for a “metabolic migraine” subtype. Additionally, these analyses identified low-cost and easily accessible biomarkers that could guide recruitment in future research on this subgroup of patients.This study is part of the trial registration: ClinicalTrials.gov: NCT03132233, registered on 27.04.2017, https://clinicaltrials.gov/ct2/show/NCT03132233