bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1
Authors: Wang Yalong , Zhu Ying , Li Wanru , Yan Shuxin , Li Chao , Ma Kunpeng , Hu Meiqin , Du Cuilian , Fu Lei , Sun Jianyuan , Zhang Claire Xi 
Published: July 2023

Synaptic vesicle (SV) endocytosis is a critical and well-regulated process for the maintenance of neurotransmission. We previously reported that synaptotagmin-11 (Syt11), an essential non-Ca2+-binding Syt associated with brain diseases, inhibits neuronal endocytosis (Wang et al., 2016). Here, we found that Syt11 deficiency caused accelerated SV endocytosis and vesicle recycling under sustained stimulation and led to the abnormal membrane partition of synaptic proteins in mouse hippocampal boutons of either sex. Furthermore, our study revealed that Syt11 has direct but Ca2+-independent binding with endophilin A1 (EndoA1), a membrane curvature sensor and endocytic protein recruiter, with high affinity. EndoA1-knockdown significantly reversed Syt11-KO phenotype, identifying EndoA1 as a main inhibitory target of Syt11 during SV endocytosis. The N-terminus of EndoA1 and the C2B domain of Syt11 were responsible for this interaction. A peptide (amino acids 314-336) derived from the Syt11 C2B efficiently blocked Syt11–EndoA1 binding bothin vitroandin vivo. Application of this peptide inhibited SV endocytosis in WT hippocampal neurons but not in EndoA1-knockdown neurons. Moreover, intracellular application of this peptide in mouse calyx of Held terminals of either sex effectively hampered both fast and slow SV endocytosis at physiological temperature. We thus propose that Syt11 ensures the precision of protein retrieval during SV endocytosis by inhibiting EndoA1 function at neuronal terminals.SIGNIFICANCE STATEMENTEndocytosis is a key stage of synaptic vesicle (SV) recycling. SV endocytosis retrieves vesicular membrane and protein components precisely to support sustained neurotransmission. However, the molecular mechanisms underlying the regulation of SV endocytosis remain elusive. Here, we reported that Syt11-KO accelerated SV endocytosis and impaired membrane partition of synaptic proteins. EndoA1 was identified as a main inhibitory target of Syt11 during SV endocytosis. Our study reveals a novel inhibitory mechanism of SV endocytosis in preventing hyperactivation of endocytosis, potentially safeguarding the recycling of synaptic proteins during sustained neurotransmission.
Pressure oscillation and optimization of Underway Conductivity- Temperature-Depth profiler
Authors: Liu Jie , Liu Zezheng , Chen Wanqian , Lv Jing , Sun Nan , Liu Chen , Du Libin 
Published: July 2023

Abstract

This paper analyzes the pressure oscillation phenomenon generated by the Underway Conductivity-Temperature-Depth profiler(UCTD)during the measurement process, and finds that the turbulent accumulation at the front end of the probe is the main cause of the pressure oscillation. Based on this, the probe structure is optimized. By establishing different probe structure models, designing orthogonal experiments, and using the finite element-based fluid analysis method, the flow resistance characteristics and internal flow field structure of the probe are analyzed, and the optimal solution is obtained. Then, the phenomenon of pressure oscillation is eliminated and the accuracy of measurement data is improved. The sea trial results show that the optimized probe structure can make the distribution of the flow field more uniform, eliminate the phenomenon of pressure oscillation, and effectively improve the validity and accuracy of data measurement.
Sea Cucumber Viscera Contains Novel Non-holostane-type Glycoside Toxins that Possess a Putative Chemical Defense Function
Authors: Liu Yanfang , Lu Zhen , Yan Zhi , Lin Ainuo , Han Shaoshuai , Li Yaxi , Yang Xiao , Li Xiaodong , Yin Xiuli , Zhang Ranran , Li Ke 
Published: July 2023

Abstract

Sea cucumbers frequently expel their guts in response to predators and an aversive environment, a behavior perceived as releasing repellents involved in chemical defense mechanisms. To investigate the chemical nature of the repellent, the viscera of stressed sea cucumbers (Apostichopus japonicus) in the Yellow Sea of China were collected and chemically analyzed. Two novel non-holostane triterpene glycosides were isolated, and the chemical structures were elucidated as 3ꞵ-O-[ꞵ-D-glucopyranosyl-(1→2)-O-ꞵ-D- xylopyranosyl]-(20S)-hydroxylanosta-7,25-diene-18(16)-lactone (1) and 3ꞵ-O-[ꞵ-D-quinovopyranosyl-(1→2)-O-ꞵ-D-xylopyranosyl]-(20S)-hydroxylanosta-7,25-diene-18(16)-lactone (2) by spectroscopic and mass-spectrometric analyses, exemplifying a triterpene glycoside constituent of an oligosaccharide containing two sugar-units and a non-holostane aglycone. Zebrafish embryos were exposed to various doses of 1 and 2 from 4 dpf hours post fertilization to 96 dpf. Compound 1 exposure showed 96 h-LC50 41.5 µM and an increased zebrafish mortality rates in roughly in a dose- and time-dependent manner. Compound 2, with different sugar substitution, exhibited no mortality and moderate teratogenic toxicity with a 96 h-EC50 of 173.5 µM. Zebrafish embryos exhibited teratogenic effects, such as reduced hatchability and total body length. The study found that triterpene saponin from A. japonicus viscera had acute toxicity in zebrafish embryos, indicating a potential chemical defense role in the marine ecosystem.
Waipouaite, Ca3(V4.54+V0.55+)O9[(Si2O5(OH)2][Si3O7.5(OH)1.5]·11H2O, a new polyoxovanadate mineral from the Aranga Quarry, New Zealand
Authors: Elliott Peter , Kampf Anthony R. 
Published: July 2023

Abstract
Waipouaite, Ca3(V4.54+V0.55+)O9[(Si2O5(OH)2][Si3O7.5(OH)1.5]·11H2O, is a new mineral from the Aranga Quarry, Northland Region, New Zealand. It occurs in basalt as overgrowths on thompsonite-Ca and chabazite-Ca and as inclusions within calcite and okenite. It forms dark olive green to almost black prismatic crystals to 0.3 mm in length. Crystals are transparent to translucent with a vitreous luster. The Mohs hardness is ~2, and the measured density is 2.24(2) g/cm3. The new mineral is biaxial (+), with α = 1.620(5), β = 1.622(5), γ = 1.628(5) (white light). The calculated 2V is 60.2°. Dispersion could not be observed. The optical orientation is Z = b. Pleochroism is X blue-green, Y olive green, Z olive; X &gt; Y &gt;&gt; Z. Electron microprobe analyses gave the empirical formula (based on 36 O apfu) (Ca2.90Na0.05K0.04Sr0.01)Σ3.00(V4.604+V0.445+)Σ5.04(Si4.97Al0.02)Σ4.99O21.45OH3.55·H2O11.00.
Waipouaite is monoclinic, P21/c, a = 12.843(3), b = 23.589(5), c = 11.560(2) Å, β = 115.54(3)°, V = 3160.0(13) Å3, and Z = 4. The eight strongest reflections in the X-ray powder diffraction pattern are [dobs in Å (I) (hkl)]: 11.78 (100) (020, 100), 9.54 (16) (011), 7.85 (19) (021), 6.29 (32) (031), 5.92 (31) (040), 5.22 (21) (122), 3.140 (18) (333), 2.850 (17) (180, 242). The crystal structure was refined using synchrotron single-crystal X-ray data to R1 = 6.85% for 6594 reflections with I&gt;2σI. Waipouaite is the first natural polyoxovanadosilicate and has a novel structure based on [(V4+,V5+)5O17] polyoxovana-date units, which are unique in natural and synthetic phases. Synthesis of polyoxovanadosilicates has proved to be a great challenge, and the discovery of waipouaite demonstrates that these compounds can form under natural conditions.
A method for water supply network DMA partitioning planning based on improved spectral clustering
Authors: Fang Qiansheng , Zhao Hongyu , Xie Chenlei , Chen Tao 
Published: July 2023

Abstract

Recently, the scale and complexity of water distribution networks (WDNs) have been increasing with the acceleration of urbanization process. It has become a hot research focus to use district metering area (DMA) for more efficient management and control of WDNs. This article proposes a multistage DMA planning method based on improved weighted spectral clustering and genetic algorithm, aiming to address issues such as high investment cost and large differences in the water network demand distribution. First, the actual case pipe network is transformed into an undirected weighted graph based on graph theory, and a similarity matrix is formed by combining the physical properties and hydraulic characteristics of the water network. Then, based on the similarity matrix, the weighted spectral clustering algorithm is used to preliminarily divide the WDN, and the performance of the water supply pipe network formed with different division quantities and different weighting schemes is discussed. Finally, the genetic algorithm is used to optimize the arrangement of valves and flow meters on boundary pipes to generate the final configuration of DMA. The results show that the proposed method has a significant improvement in pipe network topology, hydraulic performance index, and economy compared with the traditional DMA method.
Executive Summary: Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus
Authors: Sacks David B. , Arnold Mark , Bakris George L. , Bruns David E. , Horvath Andrea R. , Lernmark Åke , Metzger Boyd E. , Nathan David M. , Kirkman M. Sue 
Published: July 2023

BACKGROUND
Numerous laboratory tests are used in the diagnosis and management of patients with diabetes mellitus. The quality of the scientific evidence supporting the use of these assays varies substantially. An expert committee compiled evidence-based recommendations for laboratory analysis in patients with diabetes. The overall quality of the evidence and the strength of the recommendations were evaluated. The draft consensus recommendations were evaluated by invited reviewers and presented for public comment. Suggestions were incorporated as deemed appropriate by the authors (see Acknowledgments in the full version of the guideline). The guidelines were reviewed by the Evidence Based Laboratory Medicine Committee and the Board of Directors of the American Association for Clinical Chemistry and by the Professional Practice Committee of the American Diabetes Association.

CONTENT
Diabetes can be diagnosed by demonstrating increased concentrations of glucose in venous plasma or increased hemoglobin A1c (HbA1c) in the blood. Glycemic control is monitored by the patients measuring their own blood glucose with meters and/or with continuous interstitial glucose monitoring devices and also by laboratory analysis of HbA1c. The potential roles of noninvasive glucose monitoring; genetic testing; and measurement of ketones, autoantibodies, urine albumin, insulin, proinsulin, and C-peptide are addressed.

SUMMARY
The guidelines provide specific recommendations based on published data or derived from expert consensus. Several analytes are found to have minimal clinical value at the present time, and measurement of them is not recommended.
Measuring Supply Chain Performance from ESG Perspective
Authors: Stan Sebastian Emanuel , Țîțu Mihail-Aurel , Mănescu Gabriel , Ilie Florin Vasile , Rusu Maria Lucia 
Published: July 2023

Abstract
This paper aims to show the relationship between environmental, social, and governance (ESG) factors and the most common supply chain (SC) performance indicators within an organization. The main interest is to identify the challenges that organizations face when trying to gain a competitive advantage over other organizations by measuring supply chain efficiency and effectiveness. Therefore, the concepts and terms used are clarified in the first section, in a broader framework, to note their relevance in the context of the paper, but also the relationship between them. In the second section, scientific papers on this topic are briefly presented, which served as a scientific foundation and prompted us to undertake our study to contribute to the body of scientific knowledge on this little-addressed topic. In the third section of the research, we established a qualitative design, composed of qualitative data from secondary sources, which were obtained data through a literature review and analysed using the comparative-deductive method. The result is to propose a simplified model for measuring SC performance from an ESG perspective. In the conclusions, possible uses of this model are proposed, as well as future perspectives that could improve performance measurement by adding new indicators.
Manifestations of Gender Inequality on the Labour Market: Wage Gap Specificities
Authors: Florişteanu Elena 
Published: July 2023

Abstract
Even though data from the last few years on gender disparities on the labour market show significant improvements, a large number of gender-based differentiation practices can still be observed. Most of the differences noticed are not only reflected in the quality of working women’s social and working lives, but also in quality of their life after retirement. The results achieved by applying the principles of good practice in equal opportunities and treatment between men and women on the labour market can translate into benefits not only for women but also for the society and the economy as a whole. The paper examines some of the typical occurrences of gender inequality in employment relations, with specific focus on the pay gap. In order to obtain the most relevant results, databases of several recognized institutions are used for documentation and analysis, as well as the results of previous research, reports, and studies published at national and international level. I believe that a multidimensional and unified approach to this kind of problem is still needed, as the potential results can stimulate further action in this field.
Trace elements content and antioxidant capacity in honey from protected phytogeographical regions in Uruguay
Authors: Moreni Real Arnaldo , Cabrera Cristina , Cracco Pablo , Cadenazzi Mónica , Pirotti Florencia , Santos Estela 
Published: July 2023

Honey is a natural sweet substance produced by honey bees, from the nectars of plant flowers and honey dew. The components of honey vary depending on the geographical site and the existing flora. The objective of this work was to characterize Uruguayan honeys produced in different regions by composition of trace elements (Cu, Fe, Mn, Mo, Zn, I, Se), the antioxidant capacity, the total polyphenols, and the palynological profile. Sixty-six honey samples were obtained in two seasons, spring-summer and summer-autumn, from three regions of protected areas, and a fourth region associated with human activities. The results obtained show that the contents of Cu and Mn were significantly higher (P<0.05) in region C (1.785 ug/g and 4.668 ug/g). For Mo, the Q region presented 1.060 ug/g, being significantly different (P<0.05) from the other regions. The total polyphenol content from region C was significantly higher (P<0.05) (66.442 mg GAE/100g). Likewise, the antioxidant capacity presented significant differences (P<0.05) for region C in the spring-summerseason. Some species, genus or botanical families were only found in a particular region. This work allows us to affirm that honey can be differentiated by its micronutrient content associated with the total polyphenol content, and a higher antioxidant activity for certain regions, given by the floral species present in the region and the type of soil.
Cluster Analysis Approach to Identify Rock Type in Tertiary Reservoir of Khabaz Oil Field Case Study
Authors: Al Kattan Wafa , AL Jawad Sameer N. , Jomaah Haider Ashour 
Published: July 2023

Rock type identification is very important task in Reservoir characterization in order to constrict robust reservoir models. There are several approaches have been introduced to define the rock type in reservoirs and each approach should relate the geological and petrophysical properties, such that each rock type is proportional to a unique hydraulic flow unit. A hydraulic flow unit is a reservoir zone that is laterally and vertically has similar flow and bedding characteristics. According to effect of rock type in reservoir performance, many empirical and statistical approaches introduced. In this paper Cluster Analysis technique is used to identify the rock groups in tertiary reservoir for Khabaz oil field by analyses variation of petrophysical properties data that predicted by analysis of well log measurements. In tertiary reservoir four groups identified by cluster analysis technique, were each group was internally similar in petrophysical properties and different from others groups.
Comparison of Petrophysical Properties Measurement Methods in Sandston Rocks
Authors: Aljawad Mohammed Saleh , Ali Abdullah Abdulhasan , Abdulkhadim Marwah Dhahir 
Published: July 2023

This paper displays a survey about the laboratory routine core analysis study on ten sandstone core samples taken from Zubair Reservoir/West Quarna Oil Field. The Petrophysical properties of rock as porosity, permeability, grain's size, roundness and sorting, type of mineral and volumes of shales inside the samples were tested by many apparatus in the Petroleum Technology Department/ University of Technology such as OFITE BLP-530 Gas Porosimeter, PERG-200TM Gas Permeameter and liquid Permeameter, GeoSpec2 apparatus (NMR method), Scanning Electron Microscopy (SEM) and OFITE Spectral Gamma Ray Logger apparatus. By comparing all the results of porosity and permeability measured by these instruments, it is clear a significant variation in the values with the depth within same formation. The porosity by gas, liquid and NMR are varied (15.4 - 35.9) %, (4.6 - 22.3) % and (2.4- 13.5) % respectively, While the permeability by gas, liquid and NMR were altered (0 - 512) md, (0-139.6) md and (1.577 x10-6 – 492) md respectively.
Village Library Empowerment in an Effort to Increase Reading Interest in Children in Aman Damai Village, Sirapit District Langkat Regency
Authors: Siregar Onan M , Selwendri  
Published: July 2023

The low interest in children to read books is a problem that must be overcome by village apparatus in Aman Damai Village of Sirapit District. In addition, the management of the library is still very simple so that it is not able to attract the community, especially children to come, besides the lack of facilities and infrastructure. The purpose of providing solutions offered is to improve the administration and management system of village libraries in an effort to increase reading interest in children. This community service is addressed to the village apparatus as library manager and children. Methods used in achieving these goals include socialization to children and parents about the importance of reading interest in children and training in improving village apparatus skills in managing village libraries, providing books of various types such as textbooks to comic books for children. This community service is also done to train village apparatus in managing library through the provision of codes and numbers on books, drafting, to the lending system that can be done by children. The conclusion of this community service is that nowadays children visit the library more often because the available books can attract children’s interest to read. Moreover, the management and administration of library has become more organized than before.
A novel infrastructure for the continuous monitoring of soil CO2 emissions: a case study at the alto Tiberina near fault observatory in Italy
Authors: Caracausi Antonio , Camarda Marco , Chiaraluce Lauro , De Gregorio Sofia , Favara Rocco , Pisciotta Antonino 
Published: July 2023

Static and dynamic stress, along with earthquakes, can trigger the emission and migration of crustal fluids, as frequently observed on the surface and within the upper crust of tectonically active areas such as the northern Apennines of Italy. To investigate the origin of these fluids and their interconnection with the seismogenic process, we complemented The Alto Tiberina Near Fault Observatory (TABOO-NFO), a multidisciplinary monitoring infrastructure composed of a dense array of seismic, geodetic, strain, and radon sensors, with a proper geochemical network grounded on four soil CO2 flux monitoring stations and weather sensors, placed near the main vents of the superficial manifestations. The TABOO-NFO is a state-of-the-art monitoring infrastructure, which allows for studying various geophysical parameters connected to the deformation processes active along a crustal fault system dominated by the Alto Tiberina fault (ATF), which is a 60 km long normal fault dipping at a low angle (&lt;15°–20°). The region is favourable for conducting geochemical studies, as it is characterised by the presence of over-pressurised fluids trapped at certain depths and superficial manifestations associated with the emission of large quantities of fluids. After describing the theoretical framework and the technological aspects based on which we developed the geochemical monitoring network, we described the data recorded in the first months. Over the studied period, the results showed that soil CO2 flux was primarily influenced by environmental parameters, and that the selected sites received a regular supply of deep-origin CO2.
Gene expression signatures associated with chronic endometritis revealed by RNA sequencing
Authors: Oshina Kyoko , Kuroda Keiji , Nakabayashi Kazuhiko , Tomikawa Junko , Kitade Mari , Sugiyama Rikikazu , Hata Kenichiro , Itakura Atsuo 
Published: July 2023

IntroductionChronic endometritis (CE) is a persistent inflammatory condition of the endometrium characterized by the infiltration of plasma cells in the endometrial stroma. CD138 immunohistochemistry is considered to improve the CE diagnosis rate.MethodsUsing the number of CD138-positive cells equal or greater than five as a diagnostic criterion for CE, we identified 24 CE and 33 non-CE cases among women with infertility. We conducted RNA-sequencing analysis for these 57 cases in total as an attempt to elucidate the molecular pathogenesis of CE and to search for new biomarkers for CE.Results and DiscussionBy comparing CE and non-CE groups, we identified 20 genes upregulated in the endometria of CE patients, including 12 immunoglobulin-related genes and eight non-immunoglobulin genes as differentially expressed genes. The eight genes were MUC5AC, LTF, CAPN9, MESP1, ACSM1, TVP23A, ALOX15, and MZB1. By analyzing samples in the proliferative and secretory phases of the menstrual cycle separately, we also identified four additional non-immunoglobulin genes upregulated in CE endometria: CCDC13 by comparing the samples in the proliferative phase, and OVGP1, MTUS2, and CLIC6 by comparing the samples in the secretory phase. Although the genes upregulated in CE may serve as novel diagnostic markers of CE, many of them were upregulated only in a limited number of CE cases showing an extremely high number of CD138-positive cells near or over one hundred. Exceptionally, TVP23A was upregulated in the majority of CE cases regardless of the number of CD138-positive cells. The upregulation of TVP23A in the endometria of CE cases may reflect the pathophysiology of a cell-type or cell-types intrinsic to the endometrium rather than the accumulation of plasma cells. Our data, consisting of clinical and transcriptomic information for CE and non-CE cases, helped us identify gene expression signatures associated with CE.