bluuebunny/crossref_metadata_2025_split huggingface dataset:
End-of-life care for patients with cancer: Clinical, geographical, and sociocultural differences
Authors: Ostan Rita , Varani Silvia , Pannuti Francesco , Pannuti Raffaella , Biasco Guido , Bruera Eduardo 
Published: February 2023

AbstractObjectivesTimely, effective and personalized identification of the multidimensional needs in patients with advanced cancer are major goals of appropriate palliative care (PC) delivery. However, there is considerable variation in structures, processes, and patient demographics that might influence the intensity of end-of-life care. This study aims to characterize patterns in clinical and demographic characteristics at the inception point and their association with the intensity of care during the last month of life in advanced cancer patients assisted at home.MethodsCancer patients entered in home PC during 2020 in Italy were considered. The association between home PC services during the last month of life (primary outcome) and demographic data, performance status (Karnofsky Performance Score [KPS]), symptoms, and therapies at the entry was explored in this retrospective study.ResultsAmong 1,721 consecutive patients (919 in Centre-North and 802 in Centre-South Italy), patients from Centre-South were younger (p < 0.001), had worse KPS (p < 0.001), and shorter survival (p = 0.010). Patient age was inversely associated with the number of total/physician/nurses services during the last month of life (p < 0.001, p = 0.001, and p = 0.008, respectively). Patients with severe symptoms (asthenia, pain, and anxiety) at inception needed more PC services at the end of life (p = 0.026, p = 0.008, and p = 0.038, respectively). The distribution of workload differed according to the geographical area, with higher number of PC services provided by physicians (p < 0.001) in Centre-North and by nurses (p = 0.002) in Centre-South.Significance of resultsThese findings highlight major disparity in access and nature of PC in a country with universal access to health services. Studies aimed at comparing PC models among different countries should pay attention to the local heterogeneity within each health-care system.
Heatwaves Similar to the Unprecedented One in Summer 2021 Over Western North America Are Projected to Become More Frequent in a Warmer World
Authors: Dong Zizhen , Wang Lin , Xu Peiqiang , Cao Jie , Yang Ruowen 
Published: February 2023

AbstractWestern North America (WNA) experienced an unprecedented heatwave from late June to early July 2021, which lies far outside the historical range. Based on the model outputs from Coupled Model Intercomparison Project Phase 6, we find that the likelihood of a heatwave over WNA similar to the 2021 one increases with global warming. Such a heatwave is projected to occur more frequently with increased extreme temperature and shortened return period. It means that a rare event in the current climate will be a more common event in a warmer climate, especially under a high‐emission scenario like the Shared Socioeconomic Pathways 585 (SSP5‐8.5). Moreover, we show a large expansion of areas over WNA that will break the 2021 record in the future with an increasing level of emission scenario. Nevertheless, some heatwave records west of the Rocky Mountains are still difficult to break, highlighting the specific extremity of the 2021 WNA heatwave.
Concentric Traveling Ionospheric Disturbances (CTIDs) Triggered by the 2022 Tonga Volcanic Eruption
Authors: Liu Lei , Morton Y. Jade , Cheng Pin‐Hsuan , Amores Angel , Wright Corwin J. , Hoffmann Lars 
Published: February 2023

AbstractThis paper investigates concentric traveling ionospheric disturbances (CTIDs) associated with the Tonga volcanic eruption. Results show that: (a) two types of CTIDs (CTID #1 and CTID #2) were identified that traveled radially from Tonga at the speed of 610–880 m/s (acoustic‐mode) and 300–380 m/s (Lamb‐mode), respectively. CTID #1 reached 3,800 and 5,000 km away from the eruption location toward the directions of New Zealand and Australia, respectively. CTID #2 propagated persistently for ∼9 hr over New Zealand and Australia. (b) The CTID #2 wavefront changed after 08:35 UT over New Zealand, possibly due to a combination of factors including the anisotropic propagation of CTID #2, the regional geomagnetic declination, and westward‐moving Lamb waves. (c) Topside total electron content (TEC) enhancement with a magnitude over two TECu was observed from COSMIC‐2 measurements. The enhancement agrees with CTID #1 peak from nearby ground‐based TEC observations and could be related to the upward propagation of the F layer’s CTID #1 signatures.
Effect of Fluid Salinity on Reaction Rate and Molecular Hydrogen (H2) Formation During Peridotite Serpentinization at 300°C
Authors: Huang Ruifang , Shang Xiuqi , Zhao Yusheng , Sun Weidong , Liu Xi 
Published: February 2023

AbstractWe conducted hydrothermal experiments at 300°C and at pressure varying from 2.2 to 3.4 kbar to study the effect of fluid salinity on the coupling between molecular hydrogen (H2) formation and olivine serpentinization, where peridotite and olivine with 25–50 μm of starting grain sizes were reacted with pure H2O and saline solutions (0.5, 1.5, and 3.3 M NaCl). Serpentine, the main hydrous mineral in most experiments, was quantified according to calibration curves based on Fourier‐transformed infrared spectroscopy and X‐ray diffraction analyses. Compared to pure H2O, saline solutions promote the hydrothermal alteration of olivine and peridotite. For experiments with peridotite and pure H2O, 67% of reaction extent was achieved after 14 days, which increased to 89% in experiments with medium‐salinity solutions (1.5 M NaCl) over the same period. Medium‐ and high‐salinity solutions inhibit H2 formation during serpentinization, which is associated with the serpentinization of pyroxene especially clinopyroxene. The redox conditions were constrained according to the equilibrium H2,aq = H2,g, and very reducing conditions were achieved during the serpentinization of olivine and peridotite. This study is the first to show iowaite formation directly from peridotite serpentinization, indicating alkaline solutions. Thermodynamic calculations suggest that the hydrolysis of NaCl (NaCl + H2O = HCl + NaOH) may yield alkaline solutions, due to higher dissociation constants of NaOH compared to HCl. This study suggests that chlorine greatly influences the serpentinization of olivine and peridotite in natural geological settings. It also indicates that iowaite formation may not require oxidizing conditions as previously thought.
Linkage of Spring Vegetation Dipole Pattern in Mid‐High Latitude Asia to Preceding Autumn Sea Ice Over the Barents‐Laptev Seas
Authors: Xin Ning , Zhou Botao , Chen Haishan , Sun Shanlei 
Published: February 2023

AbstractBased on the normalized difference vegetation index (NDVI) and empirical orthogonal function analysis, a “northeast‐southwest” dipole pattern of spring vegetation in mid‐high latitude Asia was detected. This pattern was revealed to be associated with the autumn sea ice concentration (SIC) anomalies in the Barents‐Laptev Seas on the interannual variability. Increased autumn SIC over the Barents‐Laptev Seas tends to increase spring NDVI over the west of Lake Baikal and decrease spring NDVI in the Russian Far East. Further analyses indicated that the sea surface temperature (SST) anomalies persisting from winter to spring over the Northeast Atlantic and the Northwest Pacific play an important role in such a relationship. Increased autumn SIC over the Barents‐Laptev Seas is associated with a teleconnection pattern over the mid‐high latitudes in the following winter, accompanied by anomalous anticyclonic circulations over the Northeast Atlantic and the Northwest Pacific. Such changes in the large‐scale circulations induce downward net heat flux anomalies over the Northeast Atlantic and the Northwest Pacific, thereby contributing to the warming of SST which may persist to spring and in turn influence the atmospheric circulations. The Northeast Atlantic SST warming in spring may evoke a downstream wave train and cause positive geopotential height anomalies over the west of Lake Baikal, which increases local temperature and hence the vegetation. The Northwest Pacific SST warming in spring may lead to negative geopotential height anomalies over the Russian Far East through a meridional wave train, consequently resulting in a decrease in local temperature and hence vegetation.
Synthesis of Orbital Angular Momentum Antennas for Target Localization
Authors: Battaglia Giada M. , Isernia Tommaso , Palmeri Roberta , Morabito Andrea F. 
Published: February 2023

AbstractThe exploitation of Orbital Angular Momentum (OAM) is proposed to design circular‐support continuous aperture distributions and concentric‐ring arrays for target‐localization applications. The proposed approach allows generating a single isolated null of the field in the target direction, which is of interest with respect to common techniques leading to a line (or perpendicular lines) of power‐pattern zeroes in the spectral plane. In the presented design procedure, the optimal continuous aperture source is first identified by resorting to the most suitable OAM vortex and effective tools such as the Singular Value Decomposition of the involved operator and a smart Convex Programming optimization. Then, by exploiting fast deterministic algorithms, this continuous source is discretized into circular‐ring arrays emulating it at best. Relevant applications including equispaced arrays, sum‐difference reconfigurable arrays, and isophoric sparse arrays are presented. The proposed approach is also compared with the state‐of‐the‐art techniques and assessed through full‐wave electromagnetic simulations.
MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1
Authors: Li Qiankun , Hu Wenzhi , Huang Qilin , Yang Jie , Li Bingmin , Ma Kui , Wei Qian , Wang Yaxi , Su Jianlong , Sun Mengli , Cui Shengnan , Yang Rungong , Li Haihong , Fu Xiaobing , Zhang Cuiping 
Published: February 2023

AbstractUnhealable diabetic wounds need to be addressed with the help of newer, more efficacious strategies. Exosomes combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound treatment. Here, the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch (SFP) were demonstrated to promote diabetic wound healing. Silk fibroin binding peptide (SFBP) was screened through phage display, and SFBP-Gluc-MS2 (SGM) and pac-miR146a-pac fusion protein were constructed. The designed exosomes (SGM-Exos, miR146a-Exos, and SGM-miR146a-Exos) were isolated from the engineered placental mesenchymal stem cells (PMSCs) transduced with SGM or/and pac-miR146a-pac protein. Gluc signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of SGM-Exo. Moreover, the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in miR146a-Exos. Superior to untreated, SGM-miR146a-Exo-only treated, and SFP-only treated groups, SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation, collagen deposition, and neovascularization. The transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP treatment. Here, we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering.
Methylation and expression of glucocorticoid receptor exon-1 variants and FKBP5 in teenage suicide-completers
Authors: Rizavi Hooriyah S. , Khan Omar S. , Zhang Hui , Bhaumik Runa , Grayson Dennis R. , Pandey Ghanshyam N. 
Published: February 2023

AbstractA dysregulated hypothalamic-pituitary-adrenal (HPA) axis has repeatedly been demonstrated to play a fundamental role in psychiatric disorders and suicide, yet the mechanisms underlying this dysregulation are not clear. Decreased expression of the glucocorticoid receptor (GR) gene, which is also susceptible to epigenetic modulation, is a strong indicator of impaired HPA axis control. In the context of teenage suicide-completers, we have systematically analyzed the 5’UTR of the GR gene to determine the expression levels of all GR exon-1 transcript variants and their epigenetic state. We also measured the expression and the epigenetic state of the FK506-binding protein 51 (FKBP5/FKBP51), an important modulator of GR activity. Furthermore, steady-state DNA methylation levels depend upon the interplay between enzymes that promote DNA methylation and demethylation activities, thus we analyzed DNA methyltransferases (DNMTs), ten-eleven translocation enzymes (TETs), and growth arrest- and DNA-damage-inducible proteins (GADD45). Focusing on both the prefrontal cortex (PFC) and hippocampus, our results show decreased expression in specific GR exon-1 variants and a strong correlation of DNA methylation changes with gene expression in the PFC. FKBP5 expression is also increased in both areas suggesting a decreased GR sensitivity to cortisol binding. We also identified aberrant expression of DNA methylating and demethylating enzymes in both brain regions. These findings enhance our understanding of the complex transcriptional regulation of GR, providing evidence of epigenetically mediated reprogramming of the GR gene, which could lead to possible epigenetic influences that result in lasting modifications underlying an individual’s overall HPA axis response and resilience to stress.
Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency
Authors: Moon Byoung-San , Huang David , Gao Fan , Cai Mingyang , Lyu Guochang , Zhang Lei , Chen Jun , Lu Wange 
Published: February 2023

AbstractNuclear architecture underlies the transcriptional programs within the cell to establish cell identity. As previously demonstrated, long-range chromatin interactions of the Oct4 distal enhancer (DE) are correlated with active transcription in naïve state embryonic stem cells. Here, we identify and characterize extreme long-range interactions of the Oct4 DE through a novel CRISPR labeling technique we developed and chromosome conformation capture to identify lethal giant larvae 2 (Llgl2) and growth factor receptor-bound protein 7 (Grb7) as putative functional interacting target genes in different chromosomes. We show that the Oct4 DE directly regulates expression of Llgl2 and Grb7 in addition to Oct4. Expression of Llgl2 and Grb7 closely correlates with the pluripotent state, where knock down of either result in loss of pluripotency, and overexpression enhances somatic cell reprogramming. We demonstrated that biologically important interactions of the Oct4 DE can occur at extreme distances that are necessary for the maintenance of the pluripotent state.
Human IFT-A complex structures provide molecular insights into ciliary transport
Authors: Jiang Meiqin , Palicharla Vivek Reddy , Miller Darcie , Hwang Sun-Hee , Zhu Hanwen , Hixson Patricia , Mukhopadhyay Saikat , Sun Ji 
Published: February 2023

AbstractIntraflagellar transport (IFT) complexes, IFT-A and IFT-B, form bidirectional trains that move along the axonemal microtubules and are essential for assembling and maintaining cilia. Mutations in IFT subunits lead to numerous ciliopathies involving multiple tissues. However, how IFT complexes assemble and mediate cargo transport lacks mechanistic understanding due to missing high-resolution structural information of the holo-complexes. Here we report cryo-EM structures of human IFT-A complexes in the presence and absence of TULP3 at overall resolutions of 3.0–3.9 Å. IFT-A adopts a “lariat” shape with interconnected core and peripheral subunits linked by structurally vital zinc-binding domains. TULP3, the cargo adapter, interacts with IFT-A through its N-terminal region, and interface mutations disrupt cargo transport. We also determine the molecular impacts of disease mutations on complex formation and ciliary transport. Our work reveals IFT-A architecture, sheds light on ciliary transport and IFT train formation, and enables the rationalization of disease mutations in ciliopathies.
Single-cell RNA sequencing reveals the effects of chemotherapy on human pancreatic adenocarcinoma and its tumor microenvironment
Authors: Werba Gregor , Weissinger Daniel , Kawaler Emily A. , Zhao Ende , Kalfakakou Despoina , Dhara Surajit , Wang Lidong , Lim Heather B. , Oh Grace , Jing Xiaohong , Beri Nina , Khanna Lauren , Gonda Tamas , Oberstein Paul , Hajdu Cristina , Loomis Cynthia , Heguy Adriana , Sherman Mara H. , Lund Amanda W. , Welling Theodore H. , Dolgalev Igor , Tsirigos Aristotelis , Simeone Diane M. 
Published: February 2023

AbstractThe tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perform single-cell RNA sequencing on freshly collected human PDAC samples either before or after chemotherapy. Overall, we find a heterogeneous mixture of basal and classical cancer cell subtypes, along with distinct cancer-associated fibroblast and macrophage subpopulations. Strikingly, classical and basal-like cancer cells exhibit similar transcriptional responses to chemotherapy and do not demonstrate a shift towards a basal-like transcriptional program among treated samples. We observe decreased ligand-receptor interactions in treated samples, particularly between TIGIT on CD8 + T cells and its receptor on cancer cells, and identify TIGIT as the major inhibitory checkpoint molecule of CD8 + T cells. Our results suggest that chemotherapy profoundly impacts the PDAC TME and may promote resistance to immunotherapy.
A polymeric hydrogel electrocatalyst for direct water oxidation
Authors: Pei Zengxia , Tan Hao , Gu Jinxing , Lu Linguo , Zeng Xin , Zhang Tianqi , Wang Cheng , Ding Luyao , Cullen Patrick J. , Chen Zhongfang , Zhao Shenlong 
Published: February 2023

AbstractMetal-free electrocatalysts represent a main branch of active materials for oxygen evolution reaction (OER), but they excessively rely on functionalized conjugated carbon materials, which substantially restricts the screening of potential efficient carbonaceous electrocatalysts. Herein, we demonstrate that a mesostructured polyacrylate hydrogel can afford an unexpected and exceptional OER activity – on par with that of benchmark IrO2 catalyst in alkaline electrolyte, together with a high durability and good adaptability in various pH environments. Combined theoretical and electrokinetic studies reveal that the positively charged carbon atoms within the carboxylate units are intrinsically active toward OER, and spectroscopic operando characterizations also identify the fingerprint superoxide intermediate generated on the polymeric hydrogel backbone. This work expands the scope of metal-free materials for OER by providing a new class of polymeric hydrogel electrocatalysts with huge extension potentials.
Immunotherapy in breast cancer: an overview of current strategies and perspectives
Authors: Debien Véronique , De Caluwé Alex , Wang Xiaoxiao , Piccart-Gebhart Martine , Tuohy Vincent K. , Romano Emanuela , Buisseret Laurence 
Published: February 2023

AbstractRecent progress in immunobiology has led the way to successful host immunity enhancement against breast cancer. In triple-negative breast cancer, the combination of cancer immunotherapy based on PD-1/PD-L1 immune checkpoint inhibitors with chemotherapy was effective both in advanced and early setting phase 3 clinical trials. These encouraging results lead to the first approvals of immune checkpoint inhibitors in triple-negative breast cancer and thus offer new therapeutic possibilities in aggressive tumors and hard-to-treat populations. Furthermore, several ongoing trials are investigating combining immunotherapies involving immune checkpoint inhibitors with conventional therapies and as well as with other immunotherapeutic strategies such as cancer vaccines, CAR-T cells, bispecific antibodies, and oncolytic viruses in all breast cancer subtypes. This review provides an overview of immunotherapies currently under clinical development and updated key results from clinical trials. Finally, we discuss the challenges to the successful implementation of immune treatment in managing breast cancer and their implications for the design of future clinical trials.
pyGWBSE: a high throughput workflow package for GW-BSE calculations
Authors: Biswas Tathagata , Singh Arunima K. 
Published: February 2023

AbstractWe develop an open-source python workflow package, pyGWBSE to perform automated first-principles calculations within the GW-BSE (Bethe-Salpeter) framework. GW-BSE is a many body perturbation theory based approach to explore the quasiparticle (QP) and excitonic properties of materials. GW approximation accurately predicts bandgaps of materials by overcoming the bandgap underestimation issue of the more widely used density functional theory (DFT). BSE formalism produces absorption spectra directly comparable with experimental observations. pyGWBSE package achieves complete automation of the entire multi-step GW-BSE computation, including the convergence tests of several parameters that are crucial for the accuracy of these calculations. pyGWBSE is integrated with Wannier90, to generate QP bandstructures, interpolated using the maximally-localized wannier functions. pyGWBSE also enables the automated creation of databases of metadata and data, including QP and excitonic properties, which can be extremely useful for future material discovery studies in the field of ultra-wide bandgap semiconductors, electronics, photovoltaics, and photocatalysis.
Reinforcement learned adversarial agent (ReLAA) for active fault detection and prediction in space habitats
Authors: Overlin Matthew , Iannucci Steven , Wilkins Bradly , McBain Alexander , Provancher Jason 
Published: February 2023

AbstractWith growing interest for human space tourism in the twenty-first century, much attention has been directed to the robust engineering of Environmental Control and Life Support Systems in space habitats. The stable, reliable operation of such a habitat is partly achieved with an ability to recognize and predict faults. For these two purposes, a reinforcement learning adversarial agent (ReLAA) is utilized in this work. A ReLAA is trained with experimental data to actively recognize and predict faults. These capabilities are achieved by proposing actions that activate known faults in a system. Instead of issuing these harmful actions to the actual hardware, a digital twin of the mock space habitat is simulated to discover vulnerabilities that would lead to faulted operation. The methods developed in this work will allow for the discovery of damaging latent behavior, and the reduction of false positive and negative fault identification.