bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerrahi Tedavi Planlanan Lomber Disk Hernisi Hastalarında Geleneksel ve Tamamlayıcı Tıp Tekniklerinin Kullanımı: Anket Çalışması
Authors: KILIÇ Güven , POLAT Ömer , EŞBAH Ali Ümit 
Published: January 2023

Amaç: Lomber disk hastalığı ortaya çıkardığı kronik ve tekrarlayıcı bel ağrısı nedeniyle hastaların yaşam kalitelerini ve psikolojik durumlarını etkilemektedir. Ayrıca ciddi iş gücü ile ekonomik kayba neden olabilen bir hastalıktır. Ağrı nedeniyle son yıllarda hastalar geleneksel ve tamamlayıcı tıp tekniklerine yönelebilmektedir. Bu çalışmanın amacı lomber disk hernisi nedeni ile cerrahi uygulanacak hastalarda, geleneksel ve tamamlayıcı tıp uygulamaları (GETAT) ve non-farmakolojik yöntemler kullanma durumunun ve kullanılan yöntemlerin incelenmesidir.
Gereç ve Yöntemler: Lomber disk hernisi nedeni ile cerrahi tedavi endikasyonu konulmuş 36 hasta çalışmaya dahil edildi. Hastalar nörolojik muayene, Vizüel Analog Skalası, Karnofsky performans ölçeği ile değerlendirildi. Hastaların demografik ve klinik özellikleri ile GETAT ve non-farmakolojik yöntemler kullanma durumuna yönelik sorular yüz yüze görüşme sırasında soruldu.
Bulgular: Cerrahi endikasyon konulup çalışmaya katılan hastaların 18’i (%50) erkek ve 18’i (%50) kadın, yaş ortalaması 47,69±8,05 yıl idi. Herhangi bir GETAT yöntemi hastaların %55,6’sında tanı konulduğunda uygulatılmıştı. En sık uygulatılan GETAT yöntemi hacamattı. Fayda görmediğini bildiren hasta sayısı 27 (%75), daha kötüleştiğini bildiren hasta sayısı ise iki (%5,6) idi. Hastaların 26’sı (%72,2) bu yöntemlerin kullanılmasını önermemekteydi. GETAT uygulayan kişilerden sadece 4’ünün (%11,1) doktor olduğu öğrenildi.
Sonuç: Türk toplumunda da giderek kullanım sıklığı artan GETAT yöntemlerinin halk arasında doğru şekilde anlatılarak, bu konuda doğru bilgilenmenin sağlanmasına, sağlıkçılar tarafından uygulamaların yapılması konusunda önlemler alınmasına ihtiyaç vardır.
Fokal Asimetrik Meme Dansitelerinin Değerlendirilmesinde Tomosentezin Tanıya Katkısı
Authors: GÜÇLÜ Derya , NALDEMİR İbrahim Feyyaz , UNLU Elif Nisa , ÖNBAŞ Ömer 
Published: January 2023

Amaç: Bu çalışma ile mamografik incelemelerde fokal asimetrik dansite saptanan ve ek tetkik gerektiren olgularda tomosentezin tanıya katkısını araştırmak amaçlanmıştır.
Gereç ve Yöntemler: Hastanemiz Radyoloji Anabilim Dalı Meme Görüntüleme Ünitesine Şubat 2020-Haziran 2022 tarihleri arasında tarama ya da tanısal amaçlı dijital mamografi tetkiki yapılan ve fokal asimetrik meme dansitesi saptanan 56 olguya, ek olarak tomosentez inceleme yapıldı ve bulgular BI-RADS kullanılarak sınıflandırıldı. Hasta yaşı, meme paterni, fokal asimetik dansite bulunan meme ve kadranı kaydedildi. Sonrasında tamamlayıcı ve altın standart tetkik olarak bilateral meme ultrasonografi incelemesi yapılarak bulgular kaydedildi.
Bulgular: Dijital mamografi görüntüleme sonucunda fokal asimetrik dansite nedeniyle BI-RADS 0 olarak değerlendirilen 56 kadın olgunun ortalama yaşları 51,5 ± 8,1’dir. %12,5’i (n=7) A tipi, %42,9’u (n=24) B tipi, %41,1’i (n=23) C tipi, %3,6’sı (n=2) D tipi meme paternine sahiptir. Fokal asimetrik dansitelerin % 44,6’sı (n=25) sağ, %55,4’ü (n=31) sol memede saptanmıştır. Dijital tomosentez incelemelerinin değerlendirilmesinde, olguların % 41,1’i (n=23) BI-RADS 1, % 16,1’i (n=9) BI-RADS 2, % 21,4’ü (n=12) BI-RADS 3, 21,4’ü (n=12) BI-RADS 4 olarak sınıflanmıştır. Ultrasonografik incelemede hastaların %42,9’u (n=24) asimetrik fibroglandüler doku, %17,9’u (n=10) olası benign lezyon, % 19,6’sı (n=11) ise malign lezyon olarak değerlendirilmiştir. Histopatolojik inceleme önerilen 12 hastanın ikisi stromal fibrozis, diğer 10’u ise biri lobüler olmak üzere invaziv meme karsinomu olarak değerlendirilmiştir.
Sonuç: Bu çalışma mamografik incelemeler ile morfolojisi değerlendirilemeyen ve ek tetkik gerektiren fokal asimetrik dansitelerde tomosentezin tanısal önemini vurgulamaktadır. Tomosentez, son dekatta kullanımı giderek artan ve parankime süperpoze lezyonlarda yaşanabilecek tanı güçlüklerini çözebilecek görece yeni bir tekniktir.
Morphology of blood microbiota in healthy individuals assessed by light and electron microscopy
Authors: Tsafarova Borislava , Hodzhev Yordan , Yordanov Georgi , Tolchkov Vladimir , Kalfin Reni , Panaiotov Stefan 
Published: January 2023

IntroductionThe blood microbiome is still an enigma. The existence of blood microbiota in clinically healthy individuals was proven during the last 50 years. Indirect evidence from radiometric analysis suggested the existence of living microbial forms in erythrocytes. Recently targeted nucleic acid sequencing demonstrated rich microbial biodiversity in the blood of clinically healthy individuals. The morphology and proliferation cycle of blood microbiota in peripheral blood mononuclear cells (PBMC) isolated from freshly drawn and cultured whole blood are obscure.MethodsTo study the life cycle of blood microbiota we focused on light, and electron microscopy analysis. Peripheral blood mononuclear cells isolated from freshly drawn blood and stress-cultured lysed whole blood at 43°C in presence of vitamin K from healthy individuals were studied.ResultsHere, we demonstrated that free circulating microbiota in the PMBC fraction possess a well-defined cell wall and proliferate by budding or through a mechanism similar to the extrusion of progeny bodies. By contrast, stress-cultured lysed whole blood microbiota proliferated as cell-wall deficient microbiota by forming electron-dense or electron-transparent bodies. The electron-dense bodies proliferated by fission or produce in chains Gram-negatively stained progeny cells or enlarged and burst to release progeny cells of 180 – 200 nm size. On the other hand, electron-transparent bodies enlarged and emitted progeny cells through the membrane. A novel proliferation mechanism of blood microbiota called by us “a cell within a cell” was observed. It combines proliferation of progeny cells within a progeny cell which is growing within the “mother” cell.DiscussionThe rich biodiversity of eukaryotic and prokaryotic microbiota identified in blood by next-generation sequencing technologies and our microscopy results suggest different proliferation mechanisms in whole and cultured blood. Our documented evidence and conclusions provide a more comprehensive view of the existence of normal blood microbiota in healthy individuals.
Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases
Authors: Amend Lena , Gilbert Benoît Thomas P. , Pelczar Penelope , Böttcher Marius , Huber Samuel , Witte Torsten , Finckh Axel , Strowig Till 
Published: January 2023

IntroductionThe characterization of the influence of the microbiota on the development and drug responses during rheumatic diseases has intensified in recent years. The role of specific bacteria during disease development has become a central research question. Notably, several lines of evidence point to distinct microbes, e.g., Prevotella copri (P. copri) being targeted by antibodies in clinical phases of rheumatic diseases.MethodsIn the present study, we compiled a broad collection of human serum samples from individuals at risk of developing RA, chronic RA patients as well as patients with new-onset of rheumatic diseases. We evaluated the presence of inflammatory biomarkers in our serum collection as well as serum antibody responses against novel, genetically distinct isolates of P. copri and several oral pathobionts.ResultsOur analysis revealed the presence of increased levels of inflammatory markers already in pre-clinical and new onset rheumatoid arthritis. However, antibody reactivity against the microbes did not differ between patient groups. Yet, we observed high variability between the different P. copri strains. We found total serum IgG levels to slightly correlate with IgG antibody responses against P. copri, but no relation between the latter and presence or prevalence of P. copri in the intestine.DiscussionIn conclusion, our work underlined the importance of strain-level characterization and its consideration during further investigations of host-microbiota interactions and the development of microbiome-based therapeutic approaches for treating rheumatic diseases.
Sex and age differences in sST2 in cardiovascular disease
Authors: Beetler Danielle J. , Bruno Katelyn A. , Di Florio Damian N. , Douglass Erika J. , Shrestha Swikriti , Tschöpe Carsten , Cunningham Madeleine W. , Krejčí Jan , Bienertová-Vašků Julie , Pankuweit Sabine , McNamara Dennis M. , Jeon Eun-Seok , van Linthout Sophie , Blauwet Lori A. , Cooper Leslie T. , Fairweather DeLisa 
Published: January 2023

AimsThe goal of this study was to determine whether sex and age differences exist for soluble ST2 (sST2) for several cardiovascular diseases (CVDs).MethodsWe examined sST2 levels using an ELISA kit for myocarditis (n = 303), cardiomyopathy (n = 293), coronary artery disease (CAD) (n = 239), myocardial infarct (MI) (n = 159), and congestive heart failure (CHF) (n = 286) and compared them to controls that did not have CVDs (n = 234).ResultsMyocarditis occurred in this study in relatively young patients around age 40 while the other CVDs occurred more often in older individuals around age 60. We observed a sex difference in sST2 by age only in myocarditis patients (men aged 38, women 46, p = 0.0002), but not for other CVDs. Sera sST2 levels were significantly elevated compared to age-matched controls for all CVDs: myocarditis (p ≤ 0.0001), cardiomyopathy (p = 0.0009), CAD (p = 0.03), MI (p = 0.034), and CHF (p &lt; 0.0001) driven by elevated sST2 levels in females for all CVDs except myocarditis, which was elevated in both females (p = 0.002) and males (p ≤ 0.0001). Sex differences in sST2 levels were found for myocarditis and cardiomyopathy but no other CVDs and were higher in males (myocarditis p = 0.0035; cardiomyopathy p = 0.0047). sST2 levels were higher in women with myocarditis over 50 years of age compared to men (p = 0.0004) or women under 50 years of age (p = 0.015). In cardiomyopathy and MI patients, men over 50 had significantly higher levels of sST2 than women (p = 0.012 and p = 0.043, respectively) but sex and age differences were not detected in other CVDs. However, women with cardiomyopathy that experienced early menopause had higher sST2 levels than those who underwent menopause at a natural age range (p = 0.02).ConclusionWe found that sex and age differences in sera sST2 exist for myocarditis, cardiomyopathy, and MI, but were not observed in other CVDs including CAD and CHF. These initial findings in patients with self-reported CVDs indicate that more research is needed into sex and age differences in sST2 levels in individual CVDs.
Effect of nocturnal hypoxemia on glycemic control among diabetic Saudi patients presenting with obstructive sleep apnea
Authors: Mahmoud Mahmoud I. , Alotaibi Raed K. , Almusally Rayyan , Shafiek Hanaa , Elamin Yasir , Alhaj Ziad , AlBaker Waleed , Elfaki Alaeldin , Qutub Hatem , Albahrani Suha J. , Alabdrabalnabi Fatima M. , Al Saeed Zahra A. , Al Argan Reem , Al.Rubaish Fatima , Alqurashi Yousef D. , Jatoi Noor-Ahmed , Alharmaly Abdullah H. , Almubarak Zainab Z. , Al Said Abir H. , Albahrani Nada 
Published: January 2023

BackgroundObstructive sleep apnea (OSA) is a prevalent disease that is associated with an increased incidence of type II diabetes mellitus (DM) if left untreated. We aimed to determine the association between glycosylated hemoglobin (HbA1c) levels and both nocturnal hypoxemia and apnea-hypopnea index (AHI) among a Saudi patients with OSA.MethodsA cross-sectional study that enrolled 103 adult patients diagnosed with DM and confirmed to have OSA by full night attended polysomnography between 2018 and 2021. Those who presented with acute illness, chronic obstructive pulmonary disease (COPD)/restrictive lung diseases causing sleep-related hypoxemia, or no available HbA1c level within 6 months before polysomnography were excluded from the study. Univariate and multivariate linear regression analyses between HbA1c levels and parameters of interest were tested.ResultsSixty-seven (65%) of the studied population had uncontrolled DM (HbA1c ≥7%). In univariate regression analysis, there was a significant positive association between HbA1c, and sleep time spent with an oxygen saturation below 90% (T90), female gender, and body mass index (BMI) (p&lt;0.05) but not AHI, or associated comorbidities (p&gt;0.05). In the multivariate analysis, HbA1c was positively associated with increasing T90 (p&lt;0.05), and ODI (p&lt;0.05), but not with AHI (p&gt;0.05).ConclusionNocturnal hypoxemia could be an important factor affecting glycemic control in patients with OSA suffering from DM irrespective of the severity of both diseases.
Circulating estradiol and its biologically active metabolites in endometriosis and in relation to pain symptoms
Authors: Emond Jean-Philippe , Caron Patrick , Pušić Maja , Turcotte Véronique , Simonyan David , Vogler Andrej , Osredkar Joško , Rižner Tea Lanišnik , Guillemette Chantal 
Published: January 2023

ObjectivesEndometriosis (EM) is an estrogen-dominant inflammatory disease linked to infertility that affects women of reproductive age. EM lesions respond to hormonal signals that regulate uterine tissue growth and trigger inflammation and pain. The objective of this study was to evaluate whether estradiol (E2) and its biologically active metabolites are differentially associated with EM given their estrogenic and non-estrogenic actions including proliferative and inflammatory properties.DesignWe performed a retrospective study of 209 EM cases and 115 women without EM.MethodsPain-related outcomes were assessed using surveys with validated scales. Preoperative serum levels of estradiol (E2) and estrone (E1), their 2-, 4- and 16- hydroxylated (OH) and methylated (MeO) derivatives (n=16) were measured by mass spectrometry. We evaluated the associations between estrogen levels and EM anatomic sites, surgical stage, risk of EM, and symptoms reported by women. Spearman correlations established the relationships between circulating steroids.ResultsOf the sixteen estrogens profiled, eleven were detected above quantification limits in most individuals. Steroids were positively correlated, except 2-hydroxy 3MeO-E1 (2OH-3MeO-E1). Higher 2OH-3MeO-E1 was linked to an increased risk of EM (Odd ratio (OR)=1.91 (95%CI 1.09-3.34); P=0.025). Ovarian EM cases displayed enhanced 2-hydroxylation with higher 2MeO-E1 and 2OH-E1 levels (P&lt; 0.009). Abdominal, pelvic and back pain symptoms were also linked to higher 2OH-3MeO-E1 levels (OR=1.86; 95%CI 1.06-3.27; P=0.032).ConclusionsThe 2-hydroxylation pathway emerges as an unfavorable feature of EM, and is associated with ovarian EM and pain related outcomes.
Hormonal therapy is effective and safe for cryptorchidism caused by idiopathic hypogonadotropic hypogonadism in adult males
Authors: Sun Taotao , Xu Wenchao , Xu Hao , Chen Yinwei , Niu Yonghua , Wang Daoqi , Wang Tao , Yang Weimin , Liu Jihong 
Published: January 2023

BackgroundHormonal therapy is a reasonable treatment for cryptorchidism caused by idiopathic hypogonadotropic hypogonadism (IHH). However, the clinical evidence on whether it is effective and safe for the treatment of cryptorchidism caused by IHH is lacking.AimTo evaluate the effect of hormonal therapy in testicular descent, puberty development, and spermatogenesis in adult males with cryptorchidism caused by IHH.MethodsThis retrospective study included 51 patients with cryptorchidism caused by IHH from the Andrology Clinic of University affiliated teaching hospital. Patients were divided into two groups: group A patients received hormonal therapy; group B patients received surgical treatment for cryptorchidism followed by hormonal therapy.ResultsThe rate of successful testicular descent following hormonal therapy (19/32 in group A) or surgical treatment (11/19 in group B) shows no statistically significant difference. There was also no statistically significant difference in penile length, Tanner stage of pubic hair, testicular volume, and success rate of spermatogenesis between the two groups. Testicular atrophy was seen in a single patient in group B.ConclusionsHormone therapy in adult males with cryptorchidism caused by IHH is effective and safe regarding testicular descent, puberty development, and spermatogenesis. This study provides new insight into the treatment of cryptorchidism caused by IHH and highlights that hormonal therapy could be an effective, safe, and economic treatment option for cryptorchidism in males caused by IHH.
Effectiveness and safety of hydrogen inhalation as an adjunct treatment in Chinese type 2 diabetes patients: A retrospective, observational, double-arm, real-life clinical study
Authors: Zhao Ziyi , Ji Hongxiang , Zhao Yunsheng , Liu Zeyu , Sun Ruitao , Li Yuquan , Ni Tongshang 
Published: January 2023

AimTo analyze the effectiveness and safety of hydrogen inhalation (HI) therapy as an adjunct treatment in Chinese type 2 diabetes mellitus (T2DM) patients in a real-life clinical setting.MethodsThis observational, non-interventional, retrospective, double-arm, 6-month clinical study included T2DM patients receiving conventional anti-diabetes medication with or without HI initiation from 2018 to 2021. Patients were assigned to the HI group or non-HI group (control group) after 1:1 propensity score matching (PSM). The mean change in glycated hemoglobin (HbA1c) after 6 months in different groups was evaluated primarily. The secondary outcome was composed of the mean change of fasting plasma glucose (FPG), weight, lipid profile, and homeostasis model assessment. Logistics regression was performed to evaluate the likelihood of reaching different HbA1c levels after 6-month treatment between the groups. Adverse event (AE) was also evaluated in patients of both groups.ResultsIn total, 1088 patients were selected into the analysis. Compared to the control group, subjects in HI group maintained greater improvement in the level of HbA1c (-0.94% vs -0.46%), FPG (-22.7 mg/dL vs -11.7 mg/dL), total cholesterol (-12.9 mg/dL vs -4.4 mg/dL), HOMA-IR (-0.76 vs -0.17) and HOMA-β (8.2% vs 1.98%) with all p&lt; 0.001 post the treatment. Logistics regression revealed that the likelihood of reaching HbA1c&lt; 7%, ≥ 7% to&lt; 8% and &gt; 1% reduction at the follow-up period was higher in the HI group, while patients in the control group were more likely to attain HbA1c ≥ 9%. Patients in HI group was observed a lower incidence of several AEs including hypoglycemia (2.0% vs 6.8%), vomiting (2.6% vs 7.4%), constipation (1.7% vs 4.4%) and giddiness (3.3% vs 6.3%) with significance in comparison to the control group.ConclusionHI as an adjunct therapy ameliorates glycemic control, lipid metabolism, insulin resistance and AE incidence of T2DM patients after 6-month treatment, presenting a noteworthy inspiration to existing clinical diabetic treatment.
Theabrownin ameliorates liver inflammation, oxidative stress, and fibrosis in MCD diet-fed C57BL/6J mice
Authors: Zhen Qingcai , Liang Qijian , Wang Hongchun , Zheng Yan , Lu Zhongting , Bian Chunyong , Zhao Xiulan , Guo Xin 
Published: January 2023

IntroductionNonalcoholic steatohepatitis (NASH), also known as metabolic steatohepatitis, is a clinical syndrome with pathological changes like alcoholic hepatitis but without a history of excessive alcohol consumption. NASH is closely related to metabolic disorders such as obesity, insulin resistance, type 2 diabetes mellitus, and hyperlipidemia. Its main characteristics are hepatocyte steatosis with hepatocyte injury and inflammation. In severe cases, it can develop into liver cirrhosis. At present, there is no special treatment for NASH. Theabrownin (TB) is the main pigment substance in fermented tea. Theabrownin has beneficial effects on lipid metabolism and intestinal flora. However, the effect of theabrownin on NASH has not been studied. MethodsThis study was aimed at exploring the effects of theabrownin from Fuzhuan brick tea on NASH. 8-week-old mice were randomly assigned to three groups and fed with chow diet (CD), methionine and choline sufficient (MCS) diet (MCS Ctrl), which is a Methionine/choline deficient (MCD) control diet, and MCD diet. After 5 weeks of feeding, the MCD group mice were randomly divided into two groups and were gavaged with double distilled water (MCD Ctrl) or theabrownin (MCD TB) (200mg/kg body weight, dissolved in double distilled water) every day for another 4 weeks respectively, while continuing MCD diet feeding.ResultsWe found that theabrownin treatment could not improve liver mass loss and steatosis. However, theabrownin ameliorated liver injury and decreased liver inflammatory response. Theabrownin also alleviated liver oxidative stress and fibrosis. Furthermore, our results showed that theabrownin increased hepatic level of fibroblast growth factor 21 (FGF21) and reduced the phosphorylation of mitogen-activated protein kinase p38 in MCD diet-fed mice.
Construction and validation of an aging-related gene signature predicting the prognosis of pancreatic cancer
Authors: Wang Dengchuan , Zhang Yonggang , Wang Xiaokang , Zhang Limei , Xu Shi 
Published: January 2023

Background: Pancreatic cancer is a malignancy with a high mortality rate and worse prognosis. Recently, public databases and bioinformatics tools make it easy to develop the prognostic risk model of pancreatic cancer, but the aging-related risk signature has not been reported. The present study aimed to identify an aging-related risk signature with potential prognostic value for pancreatic cancer patients.Method: Gene expression profiling and human clinical information of pancreatic cancer were derived from The Cancer Genome Atlas database (TCGA). Aging-related gene sets were downloaded from The Molecular Signatures Database and aging-related genes were obtained from the Human Ageing Genomic Resources database. Firstly, Gene set enrichment analysis was carried out to investigate the role of aging process in pancreatic cancer. Secondly, differentially expressed genes and aging-related prognostic genes were screened on the basis of the overall survival information. Then, univariate COX and LASSO analysis were performed to establish an aging-related risk signature of pancreatic cancer patients. To facilitate clinical application, a nomogram was established to predict the survival rates of PCa patients. The correlations of risk score with clinical features and immune status were evaluated. Finally, potential therapeutic drugs were screened based on the connectivity map (Cmap) database and verified by molecular docking. For further validation, the protein levels of aging-related genes in normal and tumor tissues were detected in the Human Protein Atlas (HPA) database.Result: The genes of pancreatic cancer were markedly enriched in several aging-associated signaling pathways. We identified 14 key aging-related genes related to prognosis from 9,020 differentially expressed genes and establish an aging-related risk signature. This risk model indicated a strong prognostic capability both in the training set of TCGA cohort and the validation set of PACA-CA cohort and GSE62452 cohort. A nomogram combining risk score and clinical variables was built, and calibration curve and Decision curve analysis (DCA) have proved that it has a good predictive value. Additionally, the risk score was tightly linked with tumor immune microenvironment, immune checkpoints and proinflammatory factors. Moreover, a candidate drug, BRD-A47144777, was screened and verified by molecular docking, indicating this drug has the potential to treat PCa. The protein expression levels of GSK3B, SERPINE1, TOP2A, FEN1 and HIC1 were consistent with our predicted results.Conclusion: In conclusion, we identified an aging-related signature and nomogram with high prediction performance of survival and immune cell infiltration for pancreatic cancer. This signature might potentially help in providing personalized immunotherapy for patients with pancreatic cancer.