bluuebunny/crossref_metadata_2025_split huggingface dataset:
SIRT1 and ZNF350 as novel biomarkers for osteoporosis: A bioinformatics analysis and experimental validation
Authors: Zhu Naiqiang , Si Jingyuan , Hou Jingyi , Yang Ning , Chen Bin , Wei Xu , Zhu Liguo 
Published: September 2023

Abstract
Background
Osteoporosis (OP) is characterized by bone mass decrease and bone tissue microarchitectural deterioration in bone tissue. This study identified potential biomarkers for early diagnosis of OP and elucidated the mechanism of OP.
Methods
Gene expression profiles were downloaded from Gene Expression Omnibus (GEO) for the GSE56814 dataset. A gene co-expression network was constructed using weighted gene co-expression network analysis (WGCNA) to identify key modules associated with healthy and OP samples. Functional enrichment analysis was conducted using the R clusterProfiler package for modules to construct the transcriptional regulatory factor networks. We used the "ggpubr" package in R to screen for differentially expressed genes between the two samples. Gene set variation analysis (GSVA) was employed to further validate hub gene expression levels between normal and OP samples using RT-PCR and immunofluorescence to evaluate the potential biological changes in various samples.
Results
There was a distinction between the normal and OP conditions based on the preserved significant module. A total of 100 genes with the highest MM scores were considered key genes. Functional enrichment analysis suggested that the top 10 biological processes, cellular component and molecular functions were enriched. The Toll-like receptor signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, osteoclast differentiation, JAK-STAT signaling pathway, and chemokine signaling pathway were identified by Kyoto Encyclopedia of Genes and Genomes pathway analysis. SIRT1 and ZNF350 were identified by Wilcoxon algorithm as hub differentially expressed transcriptional regulatory factors that promote OP progression by affecting oxidative phosphorylation, apoptosis, PI3K-Akt-mTOR signaling, and p53 pathway. According to RT-PCR and immunostaining results, SIRT1 and ZNF350 levels were significantly higher in OP samples than in normal samples.
Conclusion
SIRT1 and ZNF350 are important transcriptional regulatory factors for the pathogenesis of OP and may be novel biomarkers for OP treatment.
Priming seeds with potassium nitrate is associated with modulation of seed germination and seedling growth ecology of cucumis metuliferus
Authors: Mutetwa Moses , Mafukidze Brian Panashe , Makaure Brenda Tsungi , Mubaiwa Tariro Clara , Dendera Obvious , Tovigepi Elvis , Mtaita Tuarira , Ngezimana Wonder , Chaibva Paul 
Published: September 2023

The horned melon (Cucumis metuliferus) is mostly grown in tropical and subtropical countries, where it thrives on deep to shallow, well-drained soils. Seed dormancy, which causes poor and delayed germination, has remained a problem for farmers in several African countries, including Zimbabwe, who have begun producing horned melon. Midlands State University conducted a laboratory experiment to evaluate the effects of potassium nitrate on seed germination and early seedling growth of horned melon. The experiment was set up in a Completely Random Design (CRD) with seven different potassium nitrate (KNO3) concentrations (0 %, 0.5 %, 1.0 %, 1.5 %, 2.0 %, 2.5 %, and 3.0 %), each duplicated three times. The data was analyzed using a one-way Analysis of Variance (ANOVA) in Genstat 18th edition. The characteristics of germination and early seedling development were assessed. All germination and early seedling development characteristics were significantly affected by potassium nitrate. Germination %, mean germination rate, germination index, mean daily germination percent, synchronization of germination, peak value for germination, and germination value all had a significant influence. Potassium nitrate had a significant influence on mean germination time and time to 50 % germination, with the lowest values obtained on seeds primed with 3 % KNO3. There was a significant difference in seedling height, root length, seedling length, fresh biomass, and dry weight between seeds primed with 2.5 % KNO3 and seeds not primed with 2.5 % KNO3. Farmers are advised to use 2.5 % KNO3 concentration for seed priming for optimal germination and early plant development of horned melon
The influence of rice protein concentrate on the technological process of wheat bread production
Authors: Shevchenko Anastasiia , Litvynchuk Svitlana , Koval Olga 
Published: September 2023

Bakery products from wheat flour are low in protein and it is inferior and also absorbed at a low level. To solve this problem sources of complete proteins may be introduced into the formulation of bakery products. But animal proteins can be allergens. An alternative can be concentrates, hydrolysates and isolates of proteins of vegeTable origin, particularly, obtained from rice. Microbiological, biochemical and conformational changes in dough and bread from wheat flour were influenced by adding rice protein concentrate. Gas-forming capacity of the dough with rice protein concentrate decreased by 8.3–20.8 % compared to the control sample where there was increase of the dosage. Gas formation occurred less intensively in the dough with rice protein concentrate, because fermentation was delayed due to a decrease in the availability of nutrients. The first peak on the gas formation graph in case of the dough with the addition of 4–8 % rice protein concentrate was seen after 65 minutes, in the control sample it was after 60 minutes. When adding 16 % of the additive, the first peak of gas formation was not clearly defined. The second peak of gas formation for control sample was observed after 150 min and for samples with rice protein concentrate a bit later and it was not clearly defined. Infrared spectra showed that relative reflection coefficient of samples with rice protein concentrate was lower both for dough after kneading and after fermentation. The addition of 4 and 8 % rice protein concentrate did not affect the amount of formed sugars, and the addition of 16 % reduced this indicator by 1.6 %. The amount of fermented sugars decreased by 3.0–7.8 %. So, changes during the technological process of bread-making can be followed due to the obtained results for obtaining bread with high protein content
Pollution status and pollution source identification in the groundwater of Yar-Dalla in Wudil, Kano, Nigeria
Authors: Onoyima Christian Chinweuba , Onoyima Nichodemus Emeka 
Published: September 2023

The recent increase in population growth and industrialization has resulted in higher pollution loads in the environment including the groundwater, which is a vital freshwater resource. Water Quality Index (WQI) was used to assess the water quality of the study area, while multivariate statistical techniques, including Principal Component Analysis (PCA) and Cluster Analysis (CA), were used to identify possible sources of the pollutants. The results of the descriptive statistics show that pH, Chloride, Alkalinity, Nitrate, and Cu are within the WHO standard for drinking water in all the water samples, while Cl-, Cd, Cr, and Pb exceeded the allowable standard in 20 %, 30 %, 10 %, and 40 % respectively of the water samples. CA group sample locations into three distinct clusters: C1 (A, B, E, G, F, and H), C2 (C, J, and I), and C3 (D). C1 has the highest anthropogenic influence followed by C2, while C3 has the least. WQI shows that C1 is in the extremely poor class (WQI>100), C2 is in the poor class (51<WQI<75), and C3 is in the good class (26<WQI<50). The PCA yielded 3 components which explained 72.98 % of the total variance in the data set. The first Component accounts for 38.85 %. Component 2 accounts for 19.76 % of the total variance while Component 3 accounts for 14.37 % of the total variance. The groundwater of the area is mainly impacted by anthropogenic factors such as agricultural activities, domestic waste, and vehicular/traffic input
Cost-effective method for the estimation of tree crown density in urban settings using a smartphone
Authors: Sippel Ivo , Moeller Lucie , Friesen Jan 
Published: September 2023

Abstract

Urban trees provide vital ecosystem services, and assessing their health is crucial for managing urban infrastructure. Traditional methods of assessing crown density, an indicator of tree vitality, involve horizontal perspectives of unobstructed canopies. This study presents a novel method for estimating crown density in urban street trees that are surrounded by obstructing objects like buildings. The approach is based on photographs of the tree crown from defined positions using a smartphone. The method was validated on eight small-leaved lime trees in Leipzig during the 2021 vegetation period, demonstrating that crown density can be estimated by analyzing smartphone-photographs from various perspectives. The method provides data to quantify crown development and can be used to compare the vitality status of individual trees. The different perspectives are consistent in their estimates of crown density throughout the annual plateau phase of crown development. During the initial greening phase, crown photographs taken from angularly oriented positions showed a higher slope value than those taken from other positions. The method can also estimate the effect of blue-green infrastructures on tree vitality compared to regular urban tree planting methods. The approach is a practical and cost-effective tool for assessing tree vitality in spatially confined urban areas.
Crianças com Transtorno do Espectro Autista e suas dificuldades pós pandemia
Authors: Bastos Joice , Moraes Sorria Marcela , Novaes Motta Adilson 
Published: September 2023

Com o surgimento do novo coronavírus, batizado de Covid-19, medidas sanitárias precisaram ser adotadas ao redor do mundo. No Brasil, tais medidas acarretaram no fechamento de estabelecimentos comerciais, estudantis, repartições públicas e igrejas. Com isso, a sociedade precisou adaptar suas tarefas, atividades e, principalmente, suas rotinas a essa nova realidade em suas residências. Dentre os grupos sociais mais afetados e prejudicados com essas mudanças, podemos destacar os indivíduos com transtorno do espectro autista (TEA) pelo fato da mudança em sua rotina. O incômodo de ter o acesso limitado a espaços externos pode causar alguns comportamentos, como a irritabilidade com maior frequência, dentre outros. Para os pais, esse novo cenário, torna-se complexo devido à sobrecarga, diminuição na qualidade de vida e limitação de recursos para contornar a situação. Este trabalho se deu por demonstrar a adversidade enfrentada pelas famílias nesse período.
Focus. De theologische nalatenschap van ds. G. Boer
Authors: Pleizier T.T.J. 
Published: September 2023

N.a.v.:
G. Boer, Tijdbetrokken vreemdelingschap. Verzameld werk van Ds. G. Boer. Deel I. Verzameld en geannoteerd door J. van der Graaf, De Banier uitgeverij, Apeldoorn 2016, 849 p., € 59,95 (ISBN 9789462786431); G. Boer, Gegronde troost. Uitleg van de Heidelbergse Catechismus. Onder redactie van G.H. Kruijmer en G.M. Breugem, De Banier uitgeverij, Apeldoorn 2019, 746 p., € 59,95 (ISBN 9789087180591); G. Boer, Verkondiging met volmacht. Verzameld werk van Ds. G. Boer. Deel II. Verzameld door J. van der Graaf, De Banier uitgeverij, Apeldoorn 2020, 332 p., € 44,95 (ISBN 9789087183004).
Role of Individual Clinician Authority in the Implementation of Informatics Tools for Population-Based Medication Management: Qualitative Semistructured Interview Study
Authors: Ranusch Allison , Lin Ying-Jen , Dorsch Michael P , Allen Arthur L , Spoutz Patrick , Seagull F Jacob , Sussman Jeremy B , Barnes Geoffrey D 
Published: September 2023

Background
Direct oral anticoagulant (DOAC) medications are frequently associated with inappropriate prescribing and adverse events. To improve the safe use of DOACs, health systems are implementing population health tools within their electronic health record (EHR). While EHR informatics tools can help increase awareness of inappropriate prescribing of medications, a lack of empowerment (or insufficient empowerment) of nonphysicians to implement change is a key barrier.

Objective
This study examined how the individual authority of clinical pharmacists and anticoagulation nurses is impacted by and changes the implementation success of an EHR DOAC Dashboard for safe DOAC medication prescribing.

Methods
We conducted semistructured interviews with pharmacists and nurses following the implementation of the EHR DOAC Dashboard at 3 clinical sites. Interview transcripts were coded according to the key determinants of implementation success. The intersections between individual clinician authority and other determinants were examined to identify themes.

Results
A high level of individual clinician authority was associated with high levels of key facilitators for effective use of the DOAC Dashboard (communication, staffing and work schedule, job satisfaction, and EHR integration). Conversely, a lack of individual authority was often associated with key barriers to effective DOAC Dashboard use. Positive individual authority was sometimes present with a negative example of another determinant, but no evidence was found of individual authority co-occurring with a positive instance of another determinant.

Conclusions
Increased individual clinician authority is a necessary antecedent to the effective implementation of an EHR DOAC Population Management Dashboard and positively affects other aspects of implementation.

International Registered Report Identifier (IRRID)
RR2-10.1186/s13012-020-01044-5
EFL Teachers’ Burnout: Do Mobile Teaching Affordances Matter?
Authors: Hedayati-Kakhki Mahnaz , Tabatabaee-Yazdi Mona 
Published: September 2023

In recent years, integrating mobile devices and network technologies has been a critical issue in the teaching and learning process, especially within the scope of English teaching and learning. The importance of technology integration in the learning and teaching process by teachers can only be deemed effective if users can realize the intended affordances in the teaching. Teachers’ affordances, accordingly, can be defined as the relationship between the properties of an educational intervention and the characteristics of the learner that enable particular kinds of learning by individuals. On the other hand, one of the critical core barriers that EFL teachers encounter in their job is the issue of burnout. Thus, because of the increased attention to the capabilities and affordances of mobile usage among teachers, this study intended to examine the relationship between EFL teachers’ mobile affordances and their burnout as its main focus. The study also aimed at determining whether gender differences influence the teachers` mobile affordances and their burnout. To this aim, 228 EFL teachers were invited to participate in this study. Data were gathered using Mobile Teaching Affordances and Maslach Burnout Inventory, and processed, analyzed, and reported using Pearson product-moment correlations and independent samples t-test. Findings proposed teachers` perceptions of mobile affordances and their burnout had significantly and negatively correlated. Furthermore, the results indicated that there was not a significant difference between males' and females` mobile affordances, however, there was a significant difference between males and females in burnout scores favoring males.
Effect of Service Quality Dimensions on Students’ Satisfaction in Selected Chartered Universities in Kenya: A Multinomial Regression Application
Authors: Kimaita Jane Naitore , Kabue Hellen , Ochola Phares , Kiriinya Stephen Ntuara 
Published: September 2023

The study sought to establish the effects of service quality dimensions on student satisfaction in selected chartered universities in Kenya. Service quality was measured under four dimensions: service tangibility, responsiveness, reliability, and accessibility on student satisfaction. A cross-sectional descriptive research design was employed Sampling was conducted in two stages: stage one was the sampling of the universities which were units of analysis, while stage two was sampling students from the sampled universities. Primary data was collected from 400 respondents. Descriptive statistics, namely; cross-tabulations and frequency distributions, were used to summarize the data, whereas inferential statistics were used to determine the relationship’s magnitude and direction. Statistical Package for Social Sciences (SPSS) was used for data analysis. The study used the Multinomial Logistic Regression (MLR) Model. The results revealed that service tangibility and responsiveness were statistically significant among the four hypotheses tested on the main effect MLR model.
Outreach and Assistance to Students regarding Fair and Humane Law Enforcement
Authors: Hapsari Ifahda Pratama , Iskandar Hardian , Jamba Padrisan 
Published: September 2023

Law enforcement in the midst of society essentially has clear legal rules, even though they cannot be calculated mathematically (certainly), but regarding their actions, the process of proving them and solving them, and the sanctions there are clear and firm rules. If these regulations can be implemented, of course the legal problems that occur in society can be resolved and run well. However, the reality of law enforcement that exists today is that there are too many anomalies that have arisen. As a result, law enforcement does not run as it should. Responding to the problem of legal inequality in law enforcement, one should not be trapped in a negative perception of the law related to the existing law enforcement process. In other words, the perception of the law must remain neutral.

Abstrak
Sosialisasi dan Pendampingan Terhadap Mahasiswa tentang Penegakan Hukum yang Adil dan Humanis. Penegakan hukum di tengah masyarakat hakikatnya telah memiliki aturan hukum yang jelas, meskipun tidak dapat diperhitungkan secara matematis (pasti), tetapi mengenai perbuatannya, prosesnya pembuktiannya dan penyelesaiannya, dan sanksinya telah terdapat aturan yang jelas dan tegas. Apabila peraturan-peraturan tersebut dapat diterapkan, tentunya problemahukum yang terjadi di masyarakat dapat diselesaikan dan berjalan dengan baik. Namun, realita penegakan hukum yang ada saat ini terlalu banyak anomali-anomali yang muncul. Akibatnya penegakan hukum tidak berjalan sebagaimana mestinya.Menyikapi persoalan ketimpangan hukum dalam penegakan hukum, maka tidaklah boleh terjebak pada persepsi negatif terhadap hukum terkait dengan proses penegakan hukum yang ada. Dengan kata lain, persepsi terhadap hukum harus tetap netral. Sebab hukum memiliki tujuan yang mulia, yakni mewujudkankeadilan, kepastian dan kemanfaatan bagi masyarakat, namun pelaksanaan penegakan hukum dipengaruhi oleh berbagai faktor sehingga hukum tidak mencapai tujuannya yang hakiki, yakni memberikan keadilan bagi masyarakat.
Marcas de subjetividade na linguagem: o “eu” e o “tu” como elementos ativadores da interação discursiva
Authors: Lima Francisco Renato , De Carvalho Maria Angélica Freire 
Published: September 2023

A subjetividade é marcada na linguagem pela presença dos pronomes ‘eu’ e ‘tu’ em interação, construindo na instância discursiva o protagonismo do sujeito na linguagem. A partir desse pressuposto, este estudo objetiva refletir sobre como se constitui a singularidade do sujeito nas interações produzidas em situações de linguagem. Parte-se, principalmente, das contribuições teóricas da epistemologia benvenistiana (2005/2006), acerca da subjetividade na linguagem e da enunciação, a qual concebe o sujeito como partícipe do ato da linguagem. Argumentos teóricos de autores, como Bakhtin (2004/2009/2011), Ducrot (1995), Maingueneau (1998), Charaudeau (1999), são postos em diálogo com Benveniste; além de Paveau; Sarfati (2006) e Flores (2013) nas reflexões sobre as linguísticas enunciativas; conta-se, ainda, com Bronckart (2008, 2012) ao tratar do interacionismo sociodiscursivo. Em crítica à linguística da língua, as linguísticas enunciativas consideram as situações interacionais de falas entre os sujeitos autônomos no discurso, implicando a marca do sujeito no ato comunicativo, onde ele atua expondo intenções e direcionando o conteúdo de suas falas a partir da apropriação de formas específicas da língua, as quais o singularizam no ato comunicativo, assumindo a condição de sujeito, em consciência de si e do outro em construção no discurso.
A alfabetização em questão: Entrevista com Leonor Scliar-Cabra
Authors: Guaresi Ronei 
Published: September 2023

Nesta edição, a equipe da Língua Nostra teve a honra de entrevistar Leonor Scli-ar-Cabral, professora e pesquisadora emérita da Universidade Federal de Santa Catarina, reconhecida nacional e internacionalmente por trabalhos publicados no campo da Aqui-sição da Linguagem. Leonor presidiu várias associações, entre elas a Associação Brasi-leira de Linguística e a International Society of Applied Psycholinguistics. A entrevista se deu em função de sua conferência de abertura no IX Seminário de Pesquisa em Estudos Linguísticos – SPEL , ocorrido na Universidade Estadual do Sudoeste da Bahia – UESB campus Vitória da Conquista em setembro de 2017 e, ainda, em função de suas preocupações atuais com os indicadores de alfabetização do Brasil, o que resultou na publicação pela entrevistada do método Scliar de Alfabetização, método fundamentado nas neurociências e na sua larga experiência como linguista.
Bentuk dan Cara Kerja Modal Sosial dalam Menghadapi Era Ketidakpastian akibat Pandemi: Studi Kasus Desa Wisata Punten Kota Batu
Authors: Susanti Anik , Izana Nyimas Nadya , Saariman Hoiril 
Published: September 2023

Desa wisata adalah salah satu industri yang paling terdampak oleh pandemi COVID-19. Artikel ini menjelaskan tentang bentuk dan cara kerja modal sosial dalam menghadapi era ketidakpastian akibat pandemi covid-19. Digunakan logika berfikir kualitatif dengan metode deskriptif untuk menjelaskan fenomena tersebut. Informan ditentukan berdasarkan kriteria-kriteria tertentu yang terdiri dari pengelola wisata, masyarakat, pemerintah desa, tokoh adat, serta pihak terkait. Observasi, catatan lapang, wawancara, dokumentasi kegiatan dalam bentuk photo dan video, serta dokumen yang tersimpan di desa adalah metode atau alat pengumpulan data yang digunakan. Sementara analisis data digunakan model interaktif Miles, Huberman, dan Saldana. Hasil investigasi ini menunjukkan modal sosial terbentuk karena nilai-nilai yang sebelumnya telah berkembang dalam masyarakat, seperti kebiasaan tolong menolong, nilai ikhlas dalam membantu. dan peduli sesama. Nilai yang telah lama berkembang dalam masyarakat membentuk modal sosial mengikat. Sementara jaringan sosial berperan dalam menghubungkan internal masyarakat, swasta dan pemerintah. Sedangkan kepercayaan membangun kerja sama. Modal sosial masyarakat Punten yang terdiri kepercayaan, jaringan sosial dan norma-norma dapat menggerakkan penghidupan masyarakat di Desa Punten selama pandemi. Bahkan penguatan modal sosial mampu bertahan dalam kondisi sulit (resiliensi). Modal sosial berupa jaringan menjadi fasilitator untuk berkembangnya norma guna mengembangkan serta memberikan kebutuhan ekonomi masyarakat. Melalui mekanisme saling membantu, peduli sesama, dan gotong-royong yang ditunjukkan oleh masyarakat Desa Punten adalah contoh bagaimana modal sosial dapat berfungsi dalam menghadapi era ketidakpastian yang disebabkan oleh pandemi COVID-19.