bluuebunny/crossref_metadata_2025_split huggingface dataset:
SECTM1-based CAR T cells enriched with CD7-low/negative subsets exhibit efficacy in CD7-positive malignancies
Authors: Wei Wenwen , Ma Haiyan , Yang Dong , Sun Bin , Tang Jie , Zhu Yongjie , Chen Xinchuan , Huang Xiaoou , Liu Jiazhuo , Hu Zhengfei , Liu Ting , Zou Liqun , Zhao Xudong 
Published: February 2023

Abstract
CD7 has been found to be a promising chimeric antigen receptor (CAR) T cell target in several clinical trials. However, its expression on normal T cells poses additional challenges in CD7-directed CAR therapy, such as complete fratricide, contamination with malignant cells, and immune suppression due to T-cell aplasia. By taking advantage of evolved affinity between ligand and receptor, we constructed a CD7-directed CAR with the extracellular domain of SECTM1, a natural ligand of CD7, as the recognition domain. SECTM1 CAR T cells killed the majority of T cells with high CD7 expression in vitro. However, SECTM1 CAR T cells with low or negative CD7 expression survived, expanded, and showed strong cytotoxicity to CD7+ malignant cell lines and primary leukemic blasts from patients with T-cell acute lymphoblastic leukemia and acute myelogenous leukemia in vitro. It also exhibited efficacy in inhibiting xenograft tumor growth in vivo. More exploration is needed for clinical efficacy potential to patients with CD7+ malignancies.
Multi-Objective Optimization of Fused Deposition Modeling for Mechanical Properties of Biopolymer Parts Using the Grey-Taguchi Method
Authors: Kumar Kapil , Singh Hari 
Published: February 2023

AbstractThe urgent need to develop customized functional products only possible by 3D printing had realized when faced with the unavailability of medical devices like surgical instruments during the coronavirus-19 disease and the on-demand necessity to perform surgery during space missions. Biopolymers have recently been the most appropriate option for fabricating surgical instruments via 3D printing in terms of cheaper and faster processing. Among all 3D printing techniques, fused deposition modelling (FDM) is a low-cost and more rapid printing technique. This article proposes the fabrication of surgical instruments, namely, forceps and hemostat using the fused deposition modeling (FDM) process. Excellent mechanical properties are the only indicator to judge the quality of the functional parts. The mechanical properties of FDM-processed parts depend on various process parameters. These parameters are layer height, infill pattern, top/bottom pattern, number of top/bottom layers, infill density, flow, number of shells, printing temperature, build plate temperature, printing speed, and fan speed. Tensile strength and modulus of elasticity are chosen as evaluation indexes to ascertain the mechanical properties of polylactic acid (PLA) parts printed by FDM. The experiments have performed through Taguchi's L27 orthogonal array (OA). Variance analysis (ANOVA) ascertains the significance of the process parameters and their percent contributions to the evaluation indexes. Finally, as a multi-objective optimization technique, grey relational analysis (GRA) obtains an optimal set of FDM process parameters to fabricate the best parts with comprehensive mechanical properties. Scanning electron microscopy (SEM) examines the types of defects and strong bonding between rasters. The proposed research ensures the successful fabrication of functional surgical tools with substantial ultimate tensile strength (42.6 MPa) and modulus of elasticity (3274 MPa).
Factors associated with diabetes-related distress among Asian patients with poorly controlled type-2 diabetes mellitus: a cross-sectional study in primary care
Authors: Guo Xiaoxuan , Wong Pang Nee Frida , Koh Yi Ling Eileen , Tan Ngiap Chuan 
Published: February 2023

AbstractBackgroundDiabetes-related distress (DRD) is a negative emotional state related to the burden of living with diabetes mellitus. It has been associated with poor self-care and glycaemic control. This cross-sectional study aimed to examine the factors associated with DRD among urban Asian patients with poorly controlled type-2 diabetes mellitus (T2DM) in primary care in Singapore. The factors included demographics, diabetes history, medical co-morbidities, mood disorders and social history.MethodsPatients with T2DM and HbA1c of 8% or more were recruited from 2 public primary care centres in Singapore. They were administered a questionnaire survey to identify DRD based on the Problem Area In Diabetes (PAID) scale. Their anxiety and depression were screened using GAD-7 and PHQ-9, and quality of life (QOL) measured using the EQ-5D-5L. Their clinical data, including HbA1c, comorbidities and medications, were extracted from the electronic medical records.ResultsAmong the 356 subjects, the prevalence of DRD was 17.4%. DRD was significantly associated with younger age (AOR (95% CI) = 0.93 (0.89–0.97),p = 0.001), ex-smoker status (AOR (95% CI) = 22.30 (2.43–204.71),p = 0.006) and history of kidney disease (AOR (95% CI) = 3.41 (1.39–8.35),p = 0.007). Those who screened positive for depression (AOR (95% CI) = 4.98 (1.19–20.86),p = 0.028) were almost five times more likely to have DRD. Quality of life was lower among those with DRD (EQ5D index score AOR (95% CI) = 0.11 (0.01–0.97),p = 0.047), who also tended to feel that diabetes pharmacotherapy interfered with their normal life (AOR (95% CI) = 2.89 (1.38–6.08),p = 0.005).ConclusionAbout 1 in 6 patients with poorly controlled T2DM had DRD. Younger age, ex-smoker status, history of kidney disease, and those with depressive symptoms were most at risk.
Multinomial logistic regression analysis of the determinants of anaemia severity among children aged 6–59 months in Ghana: new evidence from the 2019 Malaria Indicator Survey
Authors: Klu Desmond , Atiglo Donatus Yaw , Christian Aaron Kobina 
Published: February 2023

Abstract
Background
Anaemia among children under age five is a major public health issue. Although anaemia prevalence is declining in Ghana, the severity among anaemic children is worsening. This study aims to investigate the determinants of anaemia severity among children aged 6 to 59 months in Ghana.

Method
The study utilized a weighted sample of 1,258 children with anaemia with data obtained from the 2019 Ghana Malaria Indicator Survey. The predictor variables included maternal, household child and health system characteristics. SPSS version. At the multivariate level, three different multinomial logistic models were run with selected predictor variables. All tests were conducted at the 95% confidence level.

Results
The overall anaemia prevalence among children under age five was 43.5%. Of these, 2.6% were severely anaemic, 48.5% were moderately anaemic, and 48.9% had mild anaemia. The multinomial analysis showed that maternal, household, child and health system factors significantly predicted anaemia levels among anaemic children. The results indicate that a lower likelihood of anaemia severity is likely to be found among children whose mothers belong to Pentecostal/Charismatic faith (AOR = 0.18-model I; AOR = 0.15-model III) and children who tested negative for malaria (AOR = 0.28-model II and III). Again, a higher probability of anaemia severity was found among anaemic children whose mothers were not aware of NHIS coverage of malaria (AOR = 2.41-model II, AOR = 2.60-model III). With regard to moderate anaemia level, children who belong to the poorest, poorer and middle household wealth index had a higher likelihood of being moderately anaemic compared to those in rich households. Similarly, anaemic children who were less than 12 months old (AOR = 2.21-model II, AOR = 2.29-model III) and those between the ages of 1–2 years (AOR = 1.84-model II, AOR = 1.83-model III) were more likely to have moderate anaemia levels.

Conclusion
The study findings show the importance of understanding the interrelation among different factors that influence anaemia severity among children under age five as critical in developing strategies and programmes aimed at addressing childhood anaemia.
Association of HTR3B gene polymorphisms with depression and its executive dysfunction: a case–control study
Authors: Wang Lina , Wang Miao , Zhao Chaoben , Jian Jia , Qiao Dongdong 
Published: February 2023

Abstract
Background
Previous studies have shown that depression was associated with HTR3B gene. The aim of this study was to investigate the relationship between polymorphisms of the HTR3B gene and depression and its executive dysfunction in Chinese Han population.

Methods
A total of 229 patients with depressive disorder and 202 healthy controls were enrolled. Six Single nucleotide polymorphism sites (SNPs) including rs10789970, rs4938056, rs12421126, rs1176744, rs2276305 and rs12795805 were genotyped by Snapshot. Clinical features were collected using a general demographic questionnaire. The 24-item Hamilton Depression Scale (HAMD) was used to assess the symptoms’ severity of the patients. The patients' executive function was assessed using a series of cognitive tests including Maze Test, Symbolic Coding Test, Spatial Span Inverse Order Test, Linking Test, and Emotional Management Test.

Results
The genotypic and allelic distributions of rs1176744 in HTR3B gene were significantly different (χ2 = 11.129, P = 0.004, χ2 = 9.288, P = 0.002, respectively) between patients and controls. The A allele was positively correlated with depression. The proportion of A carriers was significantly higher and that of C carriers was lower in patients than those in controls. Patients had significantly lower scores of Spatial Span Inverse Order Test in carriers of A allele at locus rs1176744 and higher scores in carriers of C alleles at locus rs1176744 and rs12795805.

Conclusions
The polymorphisms of HTR3B gene may be associated with depression in Chinese Han population. The A allele of rs1176744 may increase the risk of developing depression and executive dysfunction while C alleles of rs1176744 and rs12795805 may be the protective factors for executive dysfunction in patients with depression.
Tele-pharmacy perception, knowledge and associated factors among pharmacy students in northwest Ethiopia: an input for implementers
Authors: Tegegne Masresha Derese , Wubante Sisay Maru , Melaku Mequannent Sharew , Mengiste Nebyu Demeke , Fentahun Ashenafi , Zemene Wondwossen , Zeleke Tirualem , Walle Agmasie Damtew , Lakew Getnet Tadesse , Tareke Yonas Tsegaw , Abdi Mubarek Suleman , Alemayehu Hawariyat Mamuye , Girma Eskedar Menkir , Tilahun Gizaw Getye , Demsash Addisalem Workie , Dessie Hiwote Simane 
Published: February 2023

AbstractsBackgroundTele-pharmacy is a subset of telemedicine in which pharmacies use telecommunication technology to provide patient care. Tele-pharmacy can improve pharmaceutical care service delivery by reducing medication errors, improving access to health professionals and facilities in remote and rural areas, and minimizing adverse drug events. However, there is limited evidence regarding future pharmacists' knowledge and perceptions of the Tele-pharmacy system in Ethiopia. As a result, this study aimed to assess tele-Pharmacy perception, knowledge and associated factors among pharmacy students in Northwest Ethiopia.MethodsAn institutional-based cross-sectional study was conducted among 376 pharmacy students in Northwest Ethiopia between July 15 and August 27, 2022. A pre-tested self-administered questionnaire was used to collect data. The data were entered using Epi info version 7.0 and analyzed using SPSS version 25. Descriptive statistics, bivariable and multivariable logistic regression analysis were used to describe pharmacy students' knowledge and perceptions of Tele-pharmacy and identify associated factors. An adjusted odds ratio (OR) and a p-value with a 95% confidence interval (CI) were calculated to declare statistical significance.ResultsFrom a total of 352 participants, about 32.4% with [95% CI (27%-37%)] and 48.6% with [95% CI (43%—54%)] had good knowledge and a positive perception toward Tele-pharmacy, respectively. Being age group of 26–30 (AOR = 0.35, 95% CI: 0.17–0.68), being male (AOR = 2.38, 95% CI: 1.26–4.49), Having a CPGA of > 3.5 (AOR = 2.28, 95% CI: 1.24–4.19), Taking basic computer training (AOR = 2.00, 95% CI: 1.17–3.39), Management support (AOR = 1.84, 95% CI: 1.06–3.19) were found to be significantly associated with pharmacy students' knowledge of Tele-pharmacy. Similarly, having access to electronic devices (AOR = 3.80, 95% CI: 1.81–7.97), training related to pharmacy information systems (AOR = 6.66, 95% CI: 3.34–13.29), availability of guidelines (AOR = 2.99, 95% CI: 1.62–5.50) were found to be significantly associated with pharmacy students' perceptions of Tele-pharmacy.ConclusionThis study found that pharmacy students have limited knowledge and perceptions of the Tele-pharmacy system. A continuing Tele-pharmacy training package, incorporating pharmacy information system guidelines as part of their education, and providing managerial support could be recommended to improve pharmacy students' knowledge and perception of Tele-pharmacy.
Secondary involvement of gallbladder by acute lymphoblastic leukemia presenting clinically as cholecystitis in a young patient: a case report
Authors: Rahim Shabina , Ahmad Zubair , Chundriger Qurratulain , Ahmed Arsalan , Ali Natasha , Abdul-Ghafar Jamshid 
Published: February 2023

Abstract
Background
Primary lymphoma of the liver, gallbladder, and extrahepatic bile ducts or secondary involvement of these organs by leukemia is exceedingly rare. Patients with primary lymphoma or leukemic involvement of the biliary tract and liver often present with symptoms and signs of biliary tract obstruction or inflammation.

Case presentation
We present a case of a 24-year-old male with biliary tract symptoms who underwent laparoscopic cholecystectomy. His precholecystectomy complete blood count performed on the same morning showed 72% lymphocytes while peripheral blood smears showed approximately 15% blasts. Surgeon went ahead with the procedure. Imaging done prior to surgery showed thickened gallbladder, while the liver, biliary tract, and pancreas did not show any thickening or mass lesion. However, the liver was enlarged. Grossly, the gallbladder wall did not show any stones or discrete mass involving the wall. Instead, there was subtle thickening of the gallbladder wall due to diffuse infiltration by the leukemic infiltrate. This lymphoid population reacted with PAX-5 and TdT immunohistochemical antibodies in a diffuse manner confirming precursor B-cell origin. This patient was found to have B-lymphoblastic leukemia involving his bone marrow on further clinical and diagnostic workup. Patient responded well to chemotherapy and is currently on maintenance treatment. He is well 1.5 years after his diagnosis.

Conclusion
This case highlights a unique and rare scenario where a previously undiagnosed and unsuspected hematologic malignancy initially presented with clinical features of a chronic inflammatory condition involving an abdominal organ owing to secondary involvement by the malignant infiltrate.
Barriers and facilitators to physical activity for young adult women: a systematic review and thematic synthesis of qualitative literature
Authors: Peng Bo , Ng Johan Y. Y. , Ha Amy S. 
Published: February 2023

Abstract
Background
Physical activity (PA) has many benefits in preventing diseases and maintaining physical and mental health. Women, in particular, can benefit from regular PA. However, women’s PA did not increase over the past decade globally, and the situations faced by women are often gender-specific. Healthy young adult women's PA does not receive as much attention as older women and adolescent girls, yet, they face the same situation of low level of PA. This review aims to explore and synthesise the self-identified barriers and facilitators to young adult women's participation in PA from qualitative research studies and offer suggestions for future studies and programs designed for this population.

Methods
A systematic search was conducted in Pubmed, Web of Science, Scopus, Medline, and SPORTDiscus for studies published between January 2000 to February 2022 to identify qualitative studies on the barriers and facilitators of young adult women’s PA between ages 18 to 40. The search yielded 694 studies initially, of which 23 were included. The research quality of included studies was appraised using the Critical Appraisal Skills Programme (CASP) tool. Data were extracted and thematically analysed based on the tenets of the social-ecological model (SEM).

Results
Identified barriers and facilitators were grouped into different levels of the SEM, with the most frequently cited factors being time, body image and societal beauty standards, family duty and social support, religious and cultural norms, organisation and community facilities and environment, safety issues and physical environment. Descriptive data were thematically analysed and synthesised in line with the five levels: body image, health and beauty; multiple roles, support, and PA; religious identity, cultural identity, and PA; safety issues and women’s fears.

Conclusions
This qualitative synthesis revealed in-depth information on barriers and facilitators influencing young adult women’s PA. It highlighted that the factors young adult women face are diverse at different levels yet holistic and intertwined. Future studies on young adult women’s PA should address the social-cultural influence and would benefit from applying multilevel strategies employing the SEM model. It is critical to create an open and inclusive environment and offer more opportunities for women.

Trial registration
PROSPERO CRD42021290519.
Effects of bihemispheric transcranial direct current stimulation on motor recovery in subacute stroke patients: a double-blind, randomized sham-controlled trial
Authors: Hsu Shih-Pin , Lu Chia-Feng , Lin Bing-Fong , Tang Chih-Wei , Kuo I-Ju , Tsai Yun-An , Guo Chao-Yu , Lee Po-Lei , Shyu Kuo-Kai , Niddam David M. , Lee I-Hui 
Published: February 2023

AbstractBackgroundBihemispheric transcranial direct current stimulation (tDCS) of the primary motor cortex (M1) can simultaneously modulate bilateral corticospinal excitability and interhemispheric interaction. However, how tDCS affects subacute stroke recovery remains unclear. We investigated the effects of bihemispheric tDCS on motor recovery in subacute stroke patients.MethodsWe enrolled subacute inpatients who had first-ever ischemic stroke at subcortical regions and moderate-to-severe baseline Fugl-Meyer Assessment of Upper Extremity (FMA-UE) score 2–56. Participants between 14 and 28 days after stroke were double-blind, randomly assigned (1:1) to receive real (n = 13) or sham (n = 14) bihemispheric tDCS (with ipsilesional M1 anode and contralesional M1 cathode, 20 min, 2 mA) during task practice twice daily for 20 sessions in two weeks. Residual integrity of the ipsilesional corticospinal tract was stratified between groups. The primary efficacy outcome was the change in FMA-UE score from baseline (responder as an increase ≥ 10). The secondary measures included changes in the Action Research Arm Test (ARAT), FMA-Lower Extremity (FMA-LE) and explorative resting-state MRI functional connectivity (FC) of target regions after intervention and three months post-stroke.ResultsTwenty-seven participants completed the study without significant adverse effects. Nineteen patients (70%) had no recordable baseline motor-evoked potentials (MEP-negative) from the paretic forearm. Compared with the sham group, the real tDCS group showed enhanced improvement of FMA-UE after intervention (p < 0.01, effect sizeη2 = 0.211; responder rate: 77% vs. 36%,p = 0.031), which sustained three months post-stroke (p < 0.01), but not ARAT. Interestingly, in the MEP-negative subgroup analysis, the FMA-UE improvement remained but delayed. Additionally, the FMA-LE improvement after real tDCS was not significantly greater until three months post-stroke (p < 0.01). We found that the individual FMA-UE improvements after real tDCS were associated with bilateral intrahemispheric, rather than interhemispheric, FC strengths in the targeted cortices, while the improvements after sham tDCS were associated with predominantly ipsilesional FC changes after adjustment for age and sex (p < 0.01).ConclusionsBihemispheric tDCS during task-oriented training may facilitate motor recovery in subacute stroke patients, even with compromised corticospinal tract integrity. Further studies are warranted for tDCS efficacy and network-specific neuromodulation.Trial registration: This study is registered with ClinicalTrials.gov: (ID: NCT02731508).
ADSC-derived exosomes attenuate myocardial infarction injury by promoting miR-205-mediated cardiac angiogenesis
Authors: Wang Tingting , Li Tao , Niu Xiaolin , Hu Lang , Cheng Jin , Guo Dong , Ren He , Zhao Ran , Ji Zhaole , Liu Pengyun , Li Yan , Guo Yanjie 
Published: February 2023

Abstract
Background
Acute myocardial infarction is a major health problem and is the leading cause of death worldwide. Myocardial apoptosis induced by myocardial infarction injury is involved in the pathophysiology of heart failure. Therapeutic stem cell therapy has the potential to be an effective and favorable treatment for ischemic heart disease. Exosomes derived from stem cells have been shown to effectively repair MI injury-induced cardiomyocyte damage. However, the cardioprotective benefits of adipose tissue-derived mesenchymal stem cell (ADSC)-Exos remain unknown. This study aimed to investigate the protective effects of exosomes from ADSC on the hearts of MI-treated mice and to explore the underlying mechanisms.

Methods
Cellular and molecular mechanisms were investigated using cultured ADSCs. On C57BL/6J mice, we performed myocardial MI or sham operations and assessed cardiac function, fibrosis, and angiogenesis 4 weeks later. Mice were intramyocardially injected with ADSC-Exos or vehicle-treated ADSCs after 25 min following the MI operation.

Results
Echocardiographic experiments showed that ADSC-Exos could significantly improve left ventricular ejection fraction, whereas ADSC-Exos administration could significantly alleviate MI-induced cardiac fibrosis. Additionally, ADSC-Exos treatment has been shown to reduce cardiomyocyte apoptosis while increasing angiogenesis. Molecular experiments found that exosomes extracted from ADSCs can promote the proliferation and migration of microvascular endothelial cells, facilitate angiogenesis, and inhibit cardiomyocytes apoptosis through miRNA-205. We then transferred isolated exosomes from ADSCs into MI-induced mice and observed decreased cardiac fibrosis, increased angiogenesis, and improved cardiac function. We also observed increased apoptosis and decreased expression of hypoxia-inducible factor-1α and vascular endothelial growth factor in HMEC-1 transfected with a miRNA-205 inhibitor.

Conclusion
In summary, these findings show that ADSC-Exos can alleviate cardiac injury and promote cardiac function recovery in MI-treated mice via the miRNA-205 signaling pathway. ADSC-Exos containing miRNA205 have a promising therapeutic potential in MI-induced cardiac injury.