bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bibliometric analysis of global research trends in post-stroke depression: hotspots and frontiers
Authors: Ma Wei , Li Xiangling , Yang Yi-Bao , Zhai Yang , Xie Ting-Ting , Gong Zhi-Ying , Lan Baixin , Liu Jiao , Mo Xue-Ni 
Published: January 2023

Abstract
Background
Post-stroke depression(PSD) is one of the most common complications of stroke which affects recovery and quality of patients’ life adversely. PSD also is a significant source of burden for caregivers. Recently, the number of publications on PSD has been an increasing worldwide that means PSD has attracted more and more attention. Therefore, a more comprehensive understanding of the publications on PSD is now required. The purpose of this paper is to analyze the research status, discover the hotspots and frontiers about PSD.
Methods
The publications’ raw data was retrieved from the Web of Science Core Collection on September 19, 2022. Impact Factor (IF) and Journal Citation Reports (JCR) segments of the publications were obtained from the Web of Science website. Visualization analysis was performed on CiteSpace and VOSviewer.
Results
From 2012 to 2022, the number of citations and publications about PSD increased exponentially year by year. Finally, a total of 3268 publications related to PSD were identified. China (867) and the US (738) had the most publications; Capital Med Univ and Univ Melbourne were the most prolific institutions. Ungvari GS and Hackett ML were the most active authors and the most prolific and co-cited, respectively. Journal of stroke cerebrovascular diseases (131 articles) was the highest output. Among the 3268 publications, International journal of stroke had the most citations (43.9 times), Stroke has the most citations (2,990) and the highest H-index value. The citation burst for 8 co-cited references lasts until 2022. Finally, this paper divided the hot spots into four categories: cluster 1 (study on risk factors of PSD), cluster 2 (clinically relevant research on PSD), cluster 3 (mechanisms of PSD), and cluster 4 (the Treatment Study for PSD).
Conclusion
PSD research is one research main area worldwide. This work demonstrated the knowledge structure and the evolution of the PSD research field and predictdresearches trends through visual analysis. The study of clinical treatment and prevention of PSD will instead of the study of the mechanism in the future. This study provides reference for future research of PSD.
3-Methyladenine ameliorates surgery-induced anxiety-like behaviors in aged mice by inhibiting autophagy-induced excessive oxidative stress
Authors: Wu Song-Tao , Han Shan-Shan , Xu Xi-Ming , Sun Hai-Jun , Zhou Hua , Shang Kun , Liu Zi-Hao , Liang Shu-Juan 
Published: January 2023

Abstract
Background: Postoperative anxiety is a common surgical complication in older patients. Research has recently linked excessive autophagy to several neurological disorders, including anxiety. This study aimed to determine whether 3-Methyladenine (3-MA) administration reduced anxiety-like behaviors in a mouse model following exploratory abdominal laparotomy.
Methods: An abdominal exploratory laparotomy model of postoperative anxiety was established using male C57BL/6 mice aged 20 months. 3-MA (6, 30 and 150 mg/kg) was administered via intracerebroventricular immediately following surgery. The mice were assessed 14 days after surgery using the marble burying, elevated plus maze tests, and local field potential recording in the amygdala. The levels of expression of phosphorylated-Akt, Beclin-1, LC3B, nuclear factor erythroid 2-related factor 2 (Nrf2)-occupied regions in NeuN-positive cells, superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) were measured at 24 h after surgery.
Results: The injection of 3-MA reversed the increased number of marbles buried, decreased time spent in the open arm, and enhanced θ oscillation power after 14 days of abdominal exploratory laparotomy. In addition, administration of 3-MA reduced the ratio of phosphorylated- to total-Akt, decreased expression in Beclin-1 and LC3B, attenuated MDA levels, and increased the ratio of Nrf2-occupied areas in NeuN-positive cells, SOD activity, and GSH levels under abdominal exploratory laparotomy conditions.
Conclusions: 3-MA improved anxiety-like behaviors in aged mice undergoing abdominal exploratory laparotomy by inhibiting excessive autophagy-induced oxidative stress. These results suggest that 3-MA could be an effective treatment for postoperative anxiety.
Labor markets for health supply chain management in Rwanda: a qualitative study of stakeholder perspectives
Authors: Meier Erin , Brown Andrew N. , McHenry Bridget , Buki Ines K. Gege , Egharevba Michael , Kabatende Joseph 
Published: January 2023

Abstract
Background. Effective supply chains for health products require an adequate, skilled workforce for supply chain management (SCM). Rwanda faces challenges in human resources for SCM, including limited capacity for SCM at different levels. Understanding of the factors influencing the supply of and demand for SCM professionals in Rwanda is necessary to ensure the labor market contains an adequate workforce. This study identifies the perspectives of key stakeholders in the supply chain management sector about the factors influencing the supply of and demand for SCM professionals.
Methods. Data were collected in semi-structured, group and one-on-one interviews with 35 key stakeholders involved in the supply chain management labor market from March and April 2019. Interviewees were categorized according to their role in the labor market as system actors, functional actors involved in the supply of SCM workers, and functional actors involved in the demand for SCM workers. Interviewees were asked open-ended questions about factors influencing the demand for and the supply of SCM workers, and recommendations for improvement. Interviews were analyzed thematically. A final validation workshop was held, and the results were also reviewed by the Ministry of Health.
Results. Stakeholders agreed that skills mismatch between SCM workers’ skills and the competencies jobs require impacts the supply of workers. Lack of career structure for SCM, lack of professional definitions for health supply chain management, and SCM curricula that do not match the needs of the workplace contribute to this gap. The demand for SCM professionals is poorly defined in terms of the numbers of professionals needed and skills workers require. Financial limitations hinder demand for health SCM professionals.
Conclusions. This study adds to the understanding of factors influencing the SCM labor market in Rwanda by documenting perspectives from government ministries, professional organizations, universities, and employers from SCM organizations. Improving the SCM labor market in Rwanda and the availability of the skilled cadres required for the effective management of health supply chains in Rwanda requires a coordinated effort by the Ministry of Health, private SCM companies, professional associations, education sector, and policy makers.
Nonunion surgery of trochanteric fractures (AO 31-A2) with a sliding hip screw.
Authors: Röell Boudewijn , Leijnen Michiel , Ritchie Ewan , Timmermans Marieke 
Published: January 2023

Abstract
Introduction
Sliding hip screws (SHS) or cephallomedullary nails (CMN) are the fixation methods for proximal femur fractures (AO 31-A2). There is no consensus on the preferred treatment. 2-4% of these proximal femur fractures treated with a CMN develop a nonunion. Our objective was to review our results when replacing the CMN with a SHS in treating nonunion of trochanteric fractures.
Method
From 2013 until 2020, information was collected regarding all patients with a nonunion of a proximal femur fracture (AO 31-A2) that were initially treated with a CMN in a non-academic teaching hospital with a high volume of proximal femur fractures. All patients with a nonunion of the proximal femur fracture underwent an operation where the CMN was replaced with a SHS. Baseline characteristics were recorded, as well as union rate and complications regarding the secondary surgery.
Results
In total 15 patients were treated with removal of the CMN and placement of the SHS in the study period. 80% were female, average age was 72.3 years when primary surgery was performed. Most patients complained of pain during weight bearing due to the nonunion (N = 13). Mean time until revision surgery was 9.6 months. Consolidation of the nonunion was achieved in 93.3% of the cases. Pain relief was accomplished within 2 months. Complications were registered in 5 patients.
Conclusion
A SHS is an easy and effective secondary intervention when dealing with a nonunion of a trochanteric fracture (AO 31-A2) with high union rates without the necessity for bone grafting. Due to the higher complication rate in revision surgery, the patient should be well informed prior to surgery.
Synergistic, long-term effects of glutamate dehydrogenase 1 deficiency and mild stress on cognitive function and mPFC gene and miRNA expression
Authors: Gaisler-Salomon Inna , Asraf Kfir , Zaidan Hiba , Natoor Baylasan 
Published: January 2023

Abstract

Glutamate abnormalities in the medial prefrontal cortex (mPFC) are associated with cognitive deficits. We previously showed that homozygous deletion of glutamate dehydrogenase 1 (Glud1), a metabolic enzyme critical for glutamate metabolism, in CNS leads to schizophrenia-like behavioral abnormalities and increased mPFC glutamate; mice heterozygous for CNSGlud1deletion (C-Glud1+/−mice) showed no cognitive or molecular abnormalities. Here, we examined the protracted behavioral and molecular effects of mild injection stress on C-Glud1+/−mice. We found spatial and reversal learning deficits, as well as large-scale mPFC transcriptional changes in pathways associated with glutamate and GABA signaling, in stress-exposed C-Glud1+/−mice, but not in their stress-naïve or WT littermates. Interestingly, these effects were observed several weeks following stress exposure, and the expression levels of specific glutamatergic and GABAergic genes differentiated between high and low reversal learning performance. An increase in MiR203-5p expression immediately following stress may provide a translational regulatory mechanism to account for the delayed effect of stress exposure on cognitive function. Our findings show that chronic glutamate abnormalities interact with acute stress to induce cognitive deficits, and resonate with gene x environment theories of schizophrenia. Stress-exposed C-Glud1+/- mice may model a schizophrenia high risk population, which is uniquely sensitive to stress-related ‘trigger’ events.
Plant Leaf Species Identification Using LBHPG Feature Extraction and Machine Learning Classifier Technique
Authors: Jadhav Sachin B. 
Published: January 2023

Abstract
The paper presents the identification and classification of Indian agricultural crop species using a novel combined Local Binary Histogram Pattern of Gradient (LBHPG) image feature extraction technique. Initially, a partition of the leaf image background is done through the newly developed Fast Adaptive Fuzzy C-mean Clustering (FAFCM) technique. After that, leaf objects within the image are identified using the LBHPG method. For the classification, KNN, PNN, and SVM shallow machine learning classifiers are used for crop species identification. The performance evaluation is done by using LBP and HOG individually along with the new proposed LBHPG technique for classification using KNN, PNN, and SVM Classifiers. The performance evaluation is based on six metrics parameters of the confusion matrix, viz., accuracy, sensitivity, specificity, precision, recall, and F-measure. The experimental results show that the proposed novel LBHP feature extraction technique with PNN Classifier gives the highest accuracy of 94.58%.
The Establishment of a Recombinant Polymerase Amplification Technique for the Detection of Mouse Poxvirus
Authors: Lian Yuexiao , Zhang Mengdi , Zhu Yujun , Wu Miaoli , Huang Bihong , Xiao Li , Shi Kehang , Li Peide , Cong Feng , Wang Huanan 
Published: January 2023

Abstract
Background: Ectromelia virus (ECTV) is the pathogen of mousepox, which has a limited host range and a high mortality rate. In the past century, ECTV has affected laboratory mouse colonies worldwide. Recombinant polymerase amplification (RPA), which is widely used in virus detection, is an isothermal amplification method.
Results: In this study, a probe-based RPA detection method was established for rapid and sensitive detection of ECTV.Primers were designed for the highly conserved region of crmD gene, the main core protein of recessive poxvirus, and standard plasmids were constructed.The sensitivity of the RPA assay is 100 copies of the genome per reaction. In addition, the method showed high specificity and did not cross-react with other common mouse viruses.Therefore, the practicability of the RPA method in the field was confirmed by the detection of 52 clinical samples. The real-time RPA assay was very similar to the ECTV real-time PCR assay, with 100% agreement.
Conclusions: In conclusion, this RPA assay, especially in low-resource settings, provides a novel alternative for sensitive, simple, and specific identification of ECTV.
Relationship between different levels of ROX index and the prognosis of patients receiving high-flow nasal oxygen therapy in ICU
Authors: Chong Meng , Ma Li 
Published: January 2023

Abstract
Objective: This study aimed investigate the relationship between different levels of ROX index and the prognosis of patients with nasal high-flow oxygen therapy in intensive care unit (ICU).
Methods: In this retrospective study, we collected patients receiving high-flow nasal oxygen therapy enrolled in the intensive care unit of our hospital between 2017 and 2022 . The ROX index was calculated according to the physiological indicators of the patients on the first day of admission to the ICU, and they were divided into three subgroups: Q1 (ROX index<3.85), Q2 (ROX index 3.85-4.88) and Q3 (ROX index>4.88). Baseline clinical characteristics and clinical outcomes were compared among the three groups. The Kaplan Meier curves were drawn to compare the 28-day and 60-day survival rates of patients in the three groups, and the prognosis of patients receiving nasal high-flow oxygen therapy in the intensive care unit was further explored by subgroup analysis. COX regression analysis was used to verify the association between the three groups and these indicators.
Results: A total of 517 patients with HFNC were enrolled, including 231 patients in Q1 group, 137 patients in Q2 group and 149 patients in Q3 group. Compared with the other two groups, the Q3 group had lower body temperature, heart rate, APACHE II score,serum anion gap, procalcitonin and neutrophil ratio at the beginning of admission. The number of patients with acute respiratory failure and MODS was less than the other two groups. The number of patients using noninvasive mechanical ventilation was also less than that in the other two groups. In addition, patients in Q3 group had a lower rate of death at both 28 and 60 days than those in the Q1 and Q2 groups, and survivors used noninvasive mechanical ventilation less frequently. Subgroup analysis also showed that the lower the ROX index, the higher the mortality of HFNC patients at the subgroup level.
Conclusion: The ROX index at the beginning of intensive care unit admission can predict the prognosis of patients with high-flow nasal cannulas. The higher the ROX index level, the better the prognosis of patients.
Genomics prominent role of the PAX family in Tumorigenesis
Authors: Choudhury Shouhartha 
Published: January 2023

Abstract

The tumour in the brain is a glial cell origin. Tumour factors govern cell fate during development. The murine PAX family control the transcription of embryonic cell lineages and contribute an immense role during development. PAX1-PAX9 genes assist in the origination of neural crest in the NS (nervous system). Also, paired-box-associated genes emerged with numerous derivatives during neurogenesis. So, the tumorigenesis in CNS suggested a novel molecular signature found among factors in neurogenesis. An early study demonstrated the paired-box domain involved genes degraded by molecular and immunogenic mechanisms in tumours such as Wilms tumour, Rhabdomyosarcoma, Brain tumour, and Lymphoma. The paired-box domain-mediated genes in tumour development are the subject of rigorous investigation of proliferation and prevention of apoptotic strategy. Therefore, I performed a genome-wide analysis to prevail enlightenment on PAX domains in the mammalian genome. In this study, I observed paired-box domains containing genes in two organisms (i.e. Homo sapiens and Mus musculus). In this document, my analysis data provided that the PAX domain-containing genes promote a unique group of proto-oncogenes. Also, the analysis data suggested PAX3 (target gene) and the number of PAX domain-initiated genes in the PAX family. My observation concluded the PAX domain-containing genes control the equilibrium between growth and polarity in multi-functional cells. The normal functions of the PAX3 gene might be a feature of tumour biology. In contrast, the fusion genes (PAX3 and FOXO1) potentially target for cancer-immunotherapy.
Design of a potential Sema4A based multiepitope vaccine to combat triple-negative breast cancer: An immunoinformatic approach
Authors: Paranthaman Priyanga , Veerappapillai Shanthi 
Published: January 2023

Abstract
Immunotherapy is revamping the therapeutic strategies for TNBC owing to its higher mutational burden and tumour-associated antigens. One of the most intriguing developments in cancer immunotherapy is the focus on peptide-based cancer vaccines. Thus, the current work aims to develop an efficient peptide-based vaccine against TNBC that targets Sema4A, which has recently been identified as a major regulator of TNBC progression. Initially, the antigenic peptides derived from Sema4A were determined and evaluated based on their capability to provoke immunological responses. The assessed epitopes were then linked with a suitable adjuvant (RpfB and RpfE) and appropriate linkers (AAY, GPGPG, KK, and EAAAK) to preclude junctional immunogenicity. Eventually, docking and dynamics simulations are performed against TLR-2, TLR-4, TLR-7 and TLR-9 to assess the interaction between the vaccine construct and TLR receptors, as the TLR signalling pathway is critical in the host immune response. The developed vaccine was then exposed to in silico cloning and immune simulation analysis. The findings suggest that the designed vaccine could potentially evoke significant humoral and cellular immune responses in the intended organism. Considering these outcomes, the final multi-epitope vaccine could be employed to serve as an effective choice for TNBC management and may open new avenues for further studies.
Early maturation and hyperexcitability is a shared phenotype of cortical neurons derived from different ASD-causing mutations
Authors: Stern Shani , Hussein Yara , Tripathi Utkarsh , Choudhary Ashwani , Nayak Ritu , Peles David , Rosh Idan , Djamus Jose , Spiegel Ronen , Garin-Shkolnik Tali 
Published: January 2023

AbstractAutism Spectrum Disorder (ASD) is characterized mainly by social and sensory-motor abnormal and repetitive behavior patterns. Over 1000 genetic variants were reported to be highly penetrant and causative of ASD. Many of these mutations cause comorbidities such as epilepsy and intellectual disabilities (ID). In this study, we measured cortical neurons derived from induced pluripotent stem cells (iPSCs) of patients with four mutations in the genes GRIN2B, SHANK3, UBTF, as well as chromosomal duplication in the 7q11.23 region and compared them to neurons derived from a first degree relative without the mutation. Using a whole-cell patch-clamp, we observed that the mutant cortical neurons demonstrated hyperexcitability and early maturation compared to control lines. These changes were characterized by increased sodium currents, increased amplitude and rate of excitatory postsynaptic currents (EPSCs), and more evoked action potentials in response to current stimulation in early-stage cell development (3–5 weeks post differentiation). These changes that appeared in all the different mutant lines, together with previously reported data, indicate that an early maturation and hyperexcitability may be a convergent phenotype of ASD cortical neurons.
Cellulosic surfaces endowed with chemical reactivity by physical adsorption of functionalized polysaccharides
Authors: Bouchut Arthur , Cathala Bernard , Moreau Céline , Lecourt Michael , Petit-Conil Michel , Pettignano Asja , Bernard Julien , Charlot Aurélia , Fleury Etienne 
Published: January 2023

Abstract
A strategy to functionalize cellulosic surfaces through physical adsorption of xyloglucan (XG) and carboxymethyl cellulose (CMC) derivatives bearing allyl or alkyne groups is reported. A set of functional polymer derivatives with degrees of substitution -DS- ranging from 0.4 to 0.44 are first prepared through epoxide ring opening of allyl glycidyl ether (AGE) or propargyl glycidyl ether (PGE) under mild basic aqueous medium. Contrary to alkyne-based materials, radical copolymerization allyl-XG or -CMC derivatives with acrylamide/acrylic acid leads to the formation of hydrogels confirming thereby their reactivity. Investigations into the deposition of these derivatized polysaccharides on Whatman paper and wood pine fibers further (spraying aqueous solutions, drying and desorption step in water) show that physisorption of the chains is not altered by the DS or by the nature of the anchored groups. QCM-D measurements highlight a high affinity of Allyl-XG for cellulose surface. Confocal Raman mapping of cellulosic substrates modified with alkyne derivatives indicate that the surface covering is quantitative and that the diffusion of the chains within the substrate can reach 40 µm. This aqueous functionalization/spraying procedure is a promising method to confer new ajustable properties to a range of cellulosic substrates in an eco-sustainable manner.
Laminin fragments conjugated with perlecan’s growth factor–binding domain differentiate human induced pluripotent stem cells into skin dermal stem cells
Authors: Sugiyama-Nakagiri Yoriko , Yamashita Shiho , Taniguchi Yukimasa , Shimono Chisei , Sekiguchi Kiyotoshi 
Published: January 2023

Abstract
Deriving stem cells to regenerate full-thickness human skin is important for treating skin disorders without invasive surgical procedures. Our previous protocol to differentiate human induced pluripotent stem cells (iPSCs) into skin-derived precursor cells (SKPs) as a source of dermal stem cells employs mouse fibroblasts as feeder cells and is therefore unsuitable for clinical use. Herein, we report a feeder-free method for differentiating iPSCs into SKPs by customizing culture substrates. We immunohistochemically screened for laminins expressed in dermal papillae (DP) and explored the conditions for inducing the differentiation of iPSCs into SKPs on recombinant laminin E8 (LM-E8) fragments with or without conjugation to domain I of perlecan (PDI), which binds growth factors through heparan sulphate chains. Several LM-E8 fragments, including those of LM-111, 121, 332, 421, 511, and 521, supported iPSC differentiation into SKPs without PDI conjugation. However, the SKP yield was significantly enhanced on PDI-conjugated LM-E8 fragments. SKPs induced on PDI-conjugated LM-E8 fragments retained the gene expression patterns characteristic of SKPs, as well as the ability to differentiate into adipocytes, osteocytes, and Schwann cells, as was the case with SKPs induced on feeder cells. Thus, PDI-conjugated LM-E8 fragments are promising agents for inducing iPSC differentiation into SKPs in clinical settings.