bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy
Authors: Watari Kenji , Yamasaki Suguru , Tu Hung-Ya , Shikamura Masayuki , Kamei Tatsuya , Adachi Hideki , Tochitani Tomoaki , Kita Yasuyuki , Nakamura Aya , Ueyama Kazuki , Ono Keiichi , Morinaga Chikako , Matsuyama Take , Sho Junki , Nakamura Miyuki , Fujiwara Masayo , Hori Yoriko , Tanabe Anna , Hirai Rina , Terai Orie , Ohno Osamu , Ohara Hidetaka , Hayama Tetsuya , Ikeda Atsushi , Nukaya Daiki , Matsushita Keizo , Takahashi Masayo , Kishino Akiyoshi , Kimura Toru , Kawamata Shin , Mandai Michiko , Kuwahara Atsushi 
Published: February 2023

AbstractThree-dimensional retinal organoids (3D-retinas) are a promising graft source for transplantation therapy. We previously developed self-organizing culture for 3D-retina generation from human pluripotent stem cells (hPSCs). Here we present a quality control method and preclinical studies for tissue-sheet transplantation. Self-organizing hPSCs differentiated into both retinal and off-target tissues. Gene expression analyses identified the major off-target tissues as eye-related, cortex-like, and spinal cord-like tissues. For quality control, we developed a qPCR-based test in which each hPSC-derived neuroepithelium was dissected into two tissue-sheets: inner-central sheet for transplantation and outer-peripheral sheet for qPCR to ensure retinal tissue selection. During qPCR, tissue-sheets were stored for 3–4 days using a newly developed preservation method. In a rat tumorigenicity study, no transplant-related adverse events were observed. In retinal degeneration model rats, retinal transplants differentiated into mature photoreceptors and exhibited light responses in electrophysiology assays. These results demonstrate our rationale toward self-organizing retinal sheet transplantation therapy.
Time-varying SUVr reflects the dynamics of dopamine increases during methylphenidate challenges in humans
Authors: Tomasi Dardo , Manza Peter , Logan Jean , Shokri-Kojori Ehsan , Yonga Michele-Vera , Kroll Danielle , Feldman Dana , McPherson Katherine , Biesecker Catherine , Dennis Evan , Johnson Allison , Yuan Kai , Wang Wen-Tung , Butman John A. , Wang Gene-Jack , Volkow Nora D. 
Published: February 2023

AbstractDopamine facilitates cognition and is implicated in reward processing. Methylphenidate, a dopamine transporter blocker widely used to treat attention-deficit/hyperactivity disorder, can have rewarding and addictive effects if injected. Since methylphenidate’s brain uptake is much faster after intravenous than oral intake, we hypothesize that the speed of dopamine increases in the striatum in addition to its amplitude underly drug reward. To test this we use simulations and PET data of [11C]raclopride’s binding displacement with oral and intravenous methylphenidate challenges in 20 healthy controls. Simulations suggest that the time-varying difference in standardized uptake value ratios for [11C]raclopride between placebo and methylphenidate conditions is a proxy for the time-varying dopamine increases induced by methylphenidate. Here we show that the dopamine increase induced by intravenous methylphenidate (0.25 mg/kg) in the striatum is significantly faster than that by oral methylphenidate (60 mg), and its time-to-peak is strongly associated with the intensity of the self-report of “high”. We show for the first time that the “high” is associated with the fast dopamine increases induced by methylphenidate.
Particle entrainment and rotating convection in Enceladus’ ocean
Authors: Schoenfeld Ashley M. , Hawkins Emily K. , Soderlund Krista M. , Vance Steven D. , Leonard Erin , Yin An 
Published: February 2023

AbstractObservations from Cassini have identified nanometer-sized silica grains in Saturn’s E-ring although their origin is unclear. Tidal deformation within Enceladus’ silicate core has been predicted to generate hot hydrothermal fluids that rise from the core-ocean boundary and traverse the subsurface ocean. This raises the possibility that the particles observed by Cassini could have been produced by hydrothermal alteration and ejected via the south polar plumes. Here, we use an analytical model to quantify potential for particle entrainment in Enceladus’ ocean. We use scaling relations to characterize ocean convection and define a parameter space that enables particle entrainment. We find that both the core-ocean heat fluxes and the transport timescale necessary to drive oceanic convection and entrain particles of the observed sizes are consistent with observations and predictions from existing thermal models. We conclude that hydrothermal alteration at Enceladus’ seafloor could indeed be the source of silica particles in Saturn’s E-ring.
Ru(iv)–Ru(iv) complexes having the doubly oxido-bridged core with a bridging carbonato or hydrogencarbonato ligand
Authors: Misawa-Suzuki Tomoyo , Nagao Hirotaka 
Published: February 2023

Ru(iv)–Ru(iv) complexes having CO32− or HCO3− on the {Ru2(μ-O)2} diamond core were isolated and structurally characterized. The electrochemical and spectroscopic behaviors with those of Ru(iii)–Ru(iv) species were compared and their reactivities were reported.
Supporting Children's Communication of Choices During Inpatient Rehabilitation: Effects of a Mobile Training for Health Care Providers
Authors: Gormley Jessica , McNaughton David , Light Janice 
Published: February 2023

Purpose:
Children with complex medical and communication needs often experience extensive or frequent hospital stays and rely on augmentative and alternative communication (AAC) strategies to communicate in this environment. Health care providers seldom receive training to effectively communicate with these children, which may lead to limited participation opportunities for the child during inpatient interactions.

Method:
A pretest–posttest experimental group design was completed to evaluate the effects of a brief mobile training designed to teach providers a procedure to support children with complex communication needs to communicate choices. Each provider participated in two pretest and two posttest interactions with children with complex communication needs during naturally occurring inpatient activities. Providers in the treatment group completed the video training, whereas providers in the control group did not.

Results:
Following the training, (a) more providers offered choices to the children during hospital routines, (b) providers implemented the trained procedure with increased accuracy, and (c) the children with complex communication needs consistently communicated their choices when given the opportunity to do so. The providers rated the training as easy to use, effective, and suited to the needs of the inpatient setting.

Conclusions:
This is the first AAC training designed to promote child–provider interactions in inpatient settings that demonstrates results that are efficient, socially valid, and effective in a real-world context. Future work is needed to develop additional brief and focused AAC partner trainings to teach providers to support the participation of children with complex communication needs in health care interactions.

Supplemental Material:

https://doi.org/10.23641/asha.22029008
Usage of Heat and Moisture Exchange Devices, Virtual Visits, Masking, and Vaccinations Among People With a Laryngectomy During COVID-19
Authors: Searl Jeff , Genoa Kathryn , Fritz Alyssa , Kearney Ann , Doyle Philip C. 
Published: February 2023

Purpose:
This study described the COVID-19 risk mitigation actions of people with a total laryngectomy (TL) during the pandemic.

Method:
An online survey was completed by 215 people with a TL who lived in the United States. The survey was open from December 1, 2021, to January 15, 2022.

Results:
There was a significant increase in frequency of heat and moisture exchange (HME) device use during the pandemic compared with pre–COVID-19. Frequency of HME use was significantly greater for those who were vaccinated and those who had at least one clinical visit with their speech-language pathologist (SLP). The use of virtual visits increased from 9% pre–COVID-19 to 37% during the pandemic. Seventy percent of respondents were “satisfied” or “very satisfied” with virtual visits and 51% judged them “as good as in-person.” Eighty percent were vaccinated for COVID-19 and 75% received a booster. One third reported that they did not wear mask over the face or over the tracheostoma. Twenty percent had tested positive for COVID-19 with 70% of these people requiring hospitalization.

Conclusions:
HME use and virtual SLP visits increased during the pandemic and the vaccination rate was high among this group of respondents. Overall, there were still large percentages of people with a TL who were not using an HME, not vaccinated, and did not wear a mask. SLPs should consider reaching out directly to their TL caseload, particularly those not yet seen during the pandemic, to support uptake of COVID-19 mitigation activities specific to people with a TL as the pandemic persists.
Narrative Abilities at Age 3 Are Associated Positively With Gesture Accuracy but Negatively With Gesture Rate
Authors: Pronina Mariia , Grofulovic Jelena , Castillo Eva , Prieto Pilar , Igualada Alfonso 
Published: February 2023

Purpose:
Though the frequency of gesture use by infants has been related to the development of different language abilities in the initial stages of language acquisition, less is known about whether this frequency (or “gesture rate”) continues to correlate with language measures in later stages of language acquisition, or whether the relation to language skills also depends on the accuracy with which such gestures are produced (or reproduced). This study sets out to explore whether preschoolers' narrative abilities are related to these two variables, namely, gesture rate and gesture accuracy.

Method:
A total of 31 typically developing 3- to 4-year-old children participated in a multimodal imitation task, a context-based gesture elicitation task, and a narrative retelling task.

Results:
Results showed that there was a significant positive correlation between the children's narrative scores and their gesture accuracy scores, whereas higher rates of gesture use did not correlate with higher levels of narrative skill. Further multimodal regression analysis confirmed that gesture accuracy was a positive predictor of narrative performance, and moreover, showed that gesture rate was a negative predictor.

Conclusions:
The fact that both gesture accuracy and gesture rate are strongly and differently linked to oral language abilities supports the claim that language and gesture are highly complex systems, and that complementary measures of gesture performance can help us assess with greater granularity the relationship between gesture and language development. These findings highlight the need to use gesture during clinical assessments as an informative indicator of language development and suggest that future research should further investigate the value of multimodal programs in the treatment of language and communication disorders.
Concepts of Family Support in Stuttering: A Multicultural Perspective
Authors: Medina Angela Marie , Pareja Derek , Berlanga Estrella , Crawford Clarice , Tormo Alicia 
Published: February 2023

Purpose:
The aim of this study was to identify how people who stutter (PWS) from racially/ethnically diverse backgrounds describe and perceive the types of stuttering support they received from their family members.

Method:
A qualitative research paradigm was adopted for data collection and analysis procedures. A group of 36 participants from racially/ethnically diverse backgrounds were asked to respond to an online survey about their perceptions of family support. Data were investigated using thematic analysis procedures.

Results:
The data gave rise to 12 major themes. Types of familial support that were perceived as positive included families being patient, offering support in general, listening empathetically, seeking outside resources, and providing varying degrees of attention to their family member's stuttering. Familial support that participants described as negative included families passing judgment, teasing, and punishing their family member who stutters. Participants also negatively perceived family members telling them to slow down, finishing their sentences, and not openly discussing their stuttering. Instances of participants experiencing no positive or no negative forms of stuttering support from their families were also reported.

Conclusions:
Two primary types of stuttering support captured in the findings of this study were conversation-based and reaction-based support, both of which represented family members' actions that were perceived as positive and negative by participants. Clinical implications include the need for culturally appropriate client–parent education and for the consideration of individuals' preferences for stuttering support.
Venus cloud discontinuity in 2022
Authors: Peralta J. , Cidadão A. , Morrone L. , Foster C. , Bullock M. , Young E. F. , Garate-Lopez I. , Sánchez-Lavega A. , Horinouchi T. , Imamura T. , Kardasis E. , Yamazaki A. , Watanabe S. 
Published: February 2023

Context. First identified in 2016 by the Japan Aerospace eXploration Agency (JAXA) Akatsuki mission, the discontinuity or disruption is a recurrent wave observed to propagate over decades at the deeper clouds of Venus (47–56 km above the surface), while its absence at the top of the clouds (∼70 km) suggests that it dissipates at the upper clouds and contributes to the maintenance of the puzzling atmospheric superrotation of Venus through wave-mean flow interaction.
Aims. Taking advantage of the campaign of ground-based observations undertaken in coordination with the Akatsuki mission from December 2021 until July 2022, we undertook the longest uninterrupted monitoring of the cloud discontinuity to date to obtain a pioneering long-term characterisation of its main properties and to better constrain its recurrence and lifetime.
Methods. The dayside upper, middle, and nightside lower clouds were studied with images acquired by the Akatsuki Ultraviolet Imager (UVI), amateur observers, and SpeX at the NASA Infrared Telescope Facility (IRTF). Hundreds of images were inspected in search of the discontinuity events and to measure key properties such as its dimensions, orientation, and rotation period.
Results. We succeeded in tracking the discontinuity at the middle clouds during 109 days without interruption. The discontinuity exhibited properties nearly identical to measurements in 2016 and 2020, with an orientation of 91° ±8°, length of 4100 ± 800 km, width of 500 ± 100 km, and a rotation period of 5.11 ± 0.09 days. Ultraviolet images during 13–14 June 2022 suggest that the discontinuity may have manifested at the top of the clouds during ∼21 h as a result of an altitude change in the critical level for this wave, due to slower zonal winds.
Accounting for non-ideal mixing effects in the hydrogen-helium equation of state
Authors: Howard S. , Guillot T. 
Published: February 2023

Context. The equation of state for hydrogen and helium is fundamental for studying stars and giant planets. It has been shown that because of interactions at atomic and molecular levels, the behaviour of a mixture of hydrogen and helium cannot be accurately represented by considering these elements separately.
Aims. This paper aims at providing a simple method to account for interactions between hydrogen and helium in interior and evolution models of giant planets.
Methods. Using on the one hand ab initio simulations that involve a system of interacting hydrogen and helium particles and pure equations of state for hydrogen and helium on the other, we derived the contributions in density and entropy of the interactions between hydrogen and helium particles.
Results. We show that relative variations of up to 15% in density and entropy arise when non-ideal mixing is accounted for. These non-ideal mixing effects must be considered in interior models of giant planets based on accurate gravity field measurements, particularly in the context of variations in the helium-to-hydrogen ratio. They also affect the mass-radius relation of exoplanets. We provide a table that contains the volume and entropy of mixing as a function of pressure and temperature. This table is to be combined with pure hydrogen and pure helium equations of state to obtain an equation of state that self-consistently includes mixing effects for any hydrogen and helium mixing ratio and may be used to model the interior structure and evolution of giant planets to brown dwarfs.
Conclusions. Non-linear mixing must be included in accurate calculations of the equations of state of hydrogen and helium. Uncertainties on the equation of state still exist, however. Ab initio calculations of the behaviour of the hydrogen-helium mixture in the megabar regime for various compositions should be performed in order to gain accuracy.
A horn-coupled millimetre-wave on-chip spectrometer based on lumped-element kinetic inductance detectors
Authors: Chowdhury U. , Levy-Bertrand F. , Calvo M. , Goupy J. , Monfardini A. 
Published: February 2023

Context. Millimetre-wave astronomy is an important tool for both general astrophysics studies and cosmology. A large number of unidentified sources are being detected by the large field-of-view continuum instruments operating on large telescopes.
Aims. New smart focal planes are needed to bridge the gap between the large bandwidth continuum instruments operating on single-dish telescopes and high spectral and angular resolution interferometers (e.g. ALMA in Chile and NOEMA in France). The aim is to perform low to medium spectral resolution observations and select a lower number of potentially interesting sources (i.e. high-redshift galaxies) for further follow-up.
Methods. We have designed, fabricated, and tested an innovative on-chip spectrometer sensitive in the 85–110 GHz range. It contains 16 channels, each of which covers a frequency band of about 0.2 GHz. A conical horn antenna coupled to a slot in the ground plane collects the radiation and guides it to a millimetre-wave microstrip transmission line placed on the other side of the mono-crystalline substrate. The millimetre-wave line is coupled to a filter-bank spectrometer. Each filter is capacitively coupled to a lumped-element kinetic inductance detector (LEKID). The microstrip configuration provides the benefit of low loss, due to the mono-crystalline substrate, and protects the LEKIDs from illumination by stray un-filtered light.
Results. The prototype spectrometer exhibits a spectral resolution R = λ/Δλ ≈ 300. The optical noise equivalent power is in the low 10−16 W Hz−1/2 range for an incoming power of about 0.2 pW per channel. The device is polarisation-sensitive, with a cross-polarisation lower than 1% for the best channels.
Radiation dose-fractionation in adult Aedes aegypti mosquitoes
Authors: Yamada Hanano , Maïga Hamidou , Kraupa Carina , Somda Nanwintoum Séverin Bimbilé , Mamai Wadaka , Wallner Thomas , Bouyer Jeremy 
Published: February 2023

Balancing process efficiency and adult sterile male biological quality is one of the challenges in the success of the sterile insect technique (SIT) against insect pest populations. For the SIT against mosquitoes, many stress factors need to be taken into consideration when producing sterile males that require high biological quality to remain competitive once released in the field. Pressures of mass rearing, sex sorting, irradiation treatments, packing, transport and release including handling procedures for each step, add to the overall stress budget of the sterile male post-release. Optimizing the irradiation step to achieve maximum sterility while keeping off-target somatic damage to a minimum can significantly improve male mating competitiveness. It is therefore worth examining various protocols that have been found to be effective in other insect species, such as dose fractionation. A fully sterilizing dose of 70 Gy was administered to Aedes aegypti males as one acute dose or fractionated into either two equal doses of 35 Gy, or one low dose of 10 Gy followed by a second dose of 60 Gy. The two doses were separated by either 1- or 2-day intervals. Longevity, flight ability, and mating competitiveness tests were performed to identify beneficial effects of the various treatments. Positive effects of fractionating dose were seen in terms of male longevity and mating competitiveness. Although applying split doses generally improved male quality parameters, the benefits may not outweigh the added labor in SIT programmes for the management of mosquito vectors.
Contemporary Management and Clinical Course of Acute Pulmonary Embolism: The COPE Study
Authors: Becattini Cecilia , Agnelli Giancarlo , Maggioni Aldo P. , Dentali Francesco , Fabbri Andrea , Enea Iolanda , Pomero Fulvio , Ruggieri Maria Pia , di Lenarda Andrea , Cimini Ludovica Anna , Pepe Giuseppe , Cozzio Susanna , Lucci Donata , Gulizia Michele M. ,   on behalf of COPE Investigators
Published: February 2023

Background New diagnosis, risk stratification, and treatment strategies became recently available for patients with acute pulmonary embolism (PE) leading to changes in clinical practice and potentially influencing short-term patients' outcomes.
Research question The COntemporary management of PE (COPE) study is aimed at assessing the contemporary clinical management and outcomes in patients with acute symptomatic PE.
Study Design and Methods Prospective, noninterventional, multicenter study. The co-primary study outcomes, in-hospital and 30-day death, were reported overall and by risk categories according to the European Society of Cardiology (ESC) and American Heart Association guidelines.
Results Among 5,213 study patients, PE was confirmed by computed tomography in 96.3%. In-hospital, 289 patients underwent reperfusion (5.5%), 92.1% received parenteral anticoagulants; at discharge, 75.6% received direct oral anticoagulants and 6.7% vitamin K antagonists. In-hospital and 30-day mortalities were 3.4 and 4.8%, respectively. In-hospital death occurred in 20.3% high-risk patients (n = 177), in 4.0% intermediate-risk patients (n = 3,281), and in 0.5% low-risk patients (n = 1,702) according to ESC guidelines. Further stratification in intermediate-high and intermediate-low risk patients did not reach statistical significance, but intermediate-risk patients with sPESI > 0 alone had lower mortality compared to those with one or both among right ventricular dilation at echocardiography or increased troponin. Death or clinical deterioration occurred in 1.5, 5.0, and 9.4% of patients at low, intermediate-low, and intermediate-high risk for death according to ESC guidelines.
Conclusion For the majority of patients with PE, contemporary initial management includes risk stratification and treatment with direct oral anticoagulants. In-hospital mortality remains high in intermediate and high-risk patients calling for and informing research focused on its reduction.
Trial Registration number: NCT03631810.