bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with obstetric fistula among reproductive age women in Ethiopia: a community based case control study
Authors: Woldegebriel Ataklti Gebretsadik , Gebrehiwot Gebremedhin Gebreegziabiher , Desta Abraham Aregay , Ajemu Kiros Fenta , Berhe Asfawosen Aregay , Woldearegay Tewolde Wubayehu , Ghebremedhin Kiros Demoz , Bezabih Nega Mamo 
Published: May 2023

Abstract
Background
Obstetric fistula is a major public health concerns in Ethiopia. It is the most devastating cause of all maternal morbidities.

Method
Data from the 2016 Ethiopian Demographic Health Survey (EDHS) was analyzed. A community-based unmatched case control study was conducted. Seventy cases and 210 non cases were selected using random number table. Data were analyzed by using STATA statistical software version 14. Multivariable logistic regression model was applied to determine the factors associated with fistula.

Results
The majority of fistula cases were from rural residences. The multivariable statistical model showed that rural residence (Adjusted OR (AOR) = 5, 95% CI 4.26, 7.52), age at first marriage (AOR = 3.3, 95% CI 2.83, 4.60), poorest wealth index (AOR = 3.3, 95% CI 2.24, 5.01) and decision making for contraceptive use by husband alone (AOR = 1.3, 95% CI 1.124, 1.67) were factors significantly associated with obstetric fistula.

Conclusion
Age at first marriage, rural residence, poorest wealth index and decision making for contraceptive use by husband alone were significantly associated factors for obstetric fistula. Intervening on these factors will reduce the magnitude of obstetrics fistula. In this context there is in-need to improve on avoiding early marriage through awareness creation to the community and developing legal framework by the policymakers. Furthermore, information about the joint decision making to use contraceptives should be disseminated though mass-media and interpersonal channels.
Associations between circulating cell-free mitochondrial DNA, inflammatory markers, and cognitive and physical outcomes in community dwelling older adults
Authors: Nidadavolu Lolita S. , Feger Danielle , Chen Diefei , Wu Yuqiong , Grodstein Francine , Gross Alden L. , Bennett David A. , Walston Jeremy D. , Oh Esther S. , Abadir Peter M. 
Published: May 2023

AbstractBackgroundDementia and frailty are common age-related syndromes often linked to chronic inflammation. Identifying the biological factors and pathways that contribute to chronic inflammation is crucial for developing new therapeutic targets. Circulating cell-free mitochondrial DNA (ccf-mtDNA) has been proposed as an immune stimulator and potential predictor of mortality in acute illnesses. Dementia and frailty are both associated with mitochondrial dysfunction, impaired cellular energetics, and cell death. The size and abundance of ccf-mtDNA fragments may indicate the mechanism of cell death: long fragments typically result from necrosis, while short fragments arise from apoptosis. We hypothesize that increased levels of necrosis-associated long ccf-mtDNA fragments and inflammatory markers in serum are linked to declines in cognitive and physical function, as well as increased mortality risk.ResultsOur study of 672 community-dwelling older adults revealed that inflammatory markers (C-Reactive Protein, soluble tumor necrosis factor alpha, tumor necrosis factor alpha receptor 1 [sTNFR1], and interleukin-6 [IL-6]) positively correlated with ccf-mtDNA levels in serum. Although cross-sectional analysis revealed no significant associations between short and long ccf-mtDNA fragments, longitudinal analysis demonstrated a connection between higher long ccf-mtDNA fragments (necrosis-associated) and worsening composite gait scores over time. Additionally, increased mortality risk was observed only in individuals with elevated sTNFR1 levels.ConclusionIn a community dwelling cohort of older adults, there are cross-sectional and longitudinal associations between ccf-mtDNA and sTNFR1 with impaired physical and cognitive function and increased hazard of death. This work suggests a role for long ccf-mtDNA as a blood-based marker predictive of future physical decline.
Extracellular vesicle-microRNAs mediated response of bovine ovaries to seasonal environmental changes
Authors: Gad Ahmed , Joyce Kamryn , Menjivar Nico Graham , Heredia Daniella , Rojas Camila Santos , Tesfaye Dawit , Gonella-Diaza Angela 
Published: May 2023

Abstract
Background
Among the various seasonal environmental changes, elevated ambient temperature during the summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function and fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role in intrafollicular cellular communication by, in part, mediating the deleterious effects of heat stress (HS). Here we aimed to investigate the changes in FF-EV miRNA cargoes in beef cows in response to seasonal changes: summer (SUM) compared to the winter (WIN) season using high throughput sequencing of FF-EV-coupled miRNAs. In addition to their biological relevance, the potential mechanisms involved in the packaging and release of those miRNAs as a response to environmental HS were elucidated.

Results
Sequencing analysis revealed that an average of 6.6% of the EV-RNA mapped reads were annotated to bovine miRNAs. Interestingly, miR-148a, miR-99a-5p, miR-10b, and miR-143 were the top four miRNAs in both groups accounting for approximately 52 and 62% of the total miRNA sequence reads in the SUM and WIN groups, respectively. A group of 16 miRNAs was up-regulated and 8 miRNAs were down-regulated in the SUM compared to the WIN group. Five DE-miRNAs (miR-10a, miR-10b, miR-26a, let-7f, and miR-1246) were among the top 20 expressed miRNA lists. Sequence motif analysis revealed the appearance of two specific motifs in 13 out of the 16 upregulated miRNAs under HS conditions. Both motifs were found to be potentially bonded by specific RNA binding proteins including Y-box binding proteins (YBX1 and YBX2) and RBM42.

Conclusion
Our findings indicate that FF EV-coupled miRNA profile varies under seasonal changes. These miRNAs could be a good indicator of the cellular mechanism in mediating HS response and the potential interplay between miRNA motifs and RNA binding proteins can be one of the mechanisms governing the packaging and release of miRNAs via EVs to facilitate cellular survival.
Genomic hallmarks and therapeutic implications of G0 cell cycle arrest in cancer
Authors: Wiecek Anna J. , Cutty Stephen J. , Kornai Daniel , Parreno-Centeno Mario , Gourmet Lucie E. , Tagliazucchi Guidantonio Malagoli , Jacobson Daniel H. , Zhang Ping , Xiong Lingyun , Bond Gareth L. , Barr Alexis R. , Secrier Maria 
Published: May 2023

AbstractBackgroundTherapy resistance in cancer is often driven by a subpopulation of cells that are temporarily arrested in a non-proliferative G0 state, which is difficult to capture and whose mutational drivers remain largely unknown.ResultsWe develop methodology to robustly identify this state from transcriptomic signals and characterise its prevalence and genomic constraints in solid primary tumours. We show that G0 arrest preferentially emerges in the context of more stable, less mutated genomes which maintainTP53integrity and lack the hallmarks of DNA damage repair deficiency, while presenting increased APOBEC mutagenesis. We employ machine learning to uncover novel genomic dependencies of this process and validate the role of the centrosomal geneCEP89as a modulator of proliferation and G0 arrest capacity. Lastly, we demonstrate that G0 arrest underlies unfavourable responses to various therapies exploiting cell cycle, kinase signalling and epigenetic mechanisms in single-cell data.ConclusionsWe propose a G0 arrest transcriptional signature that is linked with therapeutic resistance and can be used to further study and clinically track this state.
Clinical utility of periodic reinterpretation of CNVs of uncertain significance: an 8-year retrospective study
Authors: Ravel Jean-Marie , Renaud Mathilde , Muller Jean , Becker Aurélie , Renard Émeline , Remen Thomas , Lefort Geneviève , Dexheimer Mylène , Jonveaux Philippe , Leheup Bruno , Bonnet Céline , Lambert Laëtitia 
Published: May 2023

Abstract

Background
Array-CGH is the first-tier genetic test both in pre- and postnatal developmental disorders worldwide. Variants of uncertain significance (VUS) represent around 10~15% of reported copy number variants (CNVs). Even though VUS reanalysis has become usual in practice, no long-term study regarding CNV reinterpretation has been reported.

Methods
This retrospective study examined 1641 CGH arrays performed over 8 years (2010–2017) to demonstrate the contribution of periodically re-analyzing CNVs of uncertain significance. CNVs were classified using AnnotSV on the one hand and manually curated on the other hand. The classification was based on the 2020 American College of Medical Genetics (ACMG) criteria.

Results
Of the 1641 array-CGH analyzed, 259 (15.7%) showed at least one CNV initially reported as of uncertain significance. After reinterpretation, 106 of the 259 patients (40.9%) changed categories, and 12 of 259 (4.6%) had a VUS reclassified to likely pathogenic or pathogenic. Six were predisposing factors for neurodevelopmental disorder/autism spectrum disorder (ASD). CNV type (gain or loss) does not seem to impact the reclassification rate, unlike the length of the CNV: 75% of CNVs downgraded to benign or likely benign are less than 500 kb in size.

Conclusions
This study’s high rate of reinterpretation suggests that CNV interpretation has rapidly evolved since 2010, thanks to the continuous enrichment of available databases. The reinterpreted CNV explained the phenotype for ten patients, leading to optimal genetic counseling. These findings suggest that CNVs should be reinterpreted at least every 2 years.
How effective are anthocyanins on healthy modification of cardiometabolic risk factors: a systematic review and meta-analysis
Authors: Neyestani Tirang R. , Yari Zahra , Rasekhi Hamid , Nikooyeh Bahareh 
Published: May 2023

Abstract
Background
In this meta-analysis, findings from recent studies on the preventive properties of anthocyanins (ACN) against cardiovascular disease are summarized.

Methods
MEDLINE, PubMed, Embase, Cochrane Library, and Google Scholar were searched and 2512 studies were found in a preliminary search. After screening of titles and abstracts, 47 studies met the inclusion criteria (randomized clinical trial design and sufficient data of outcomes). Studies were excluded based on the following criteria: incomplete data; obscurely reported outcomes, or lack of control groups; and animal studies.

Results
The results showed that intervention with ACNs resulted in a significant decrease in body mass index ((MD),− 0.21; 95% CI, − 0.38, − 0.04; P < 0.001) and body fat mass (MD: − 0.3%, 95% CI − 0.42 to − 0.18%, p < 0.001). Pooled data comparing ACN with control showed statistically significant effect on fasting blood sugar and HbA1c. However, the reductions were significantly more in the subjects with type 2 diabetes and in those who used ACN as supplement/extract. The subgroup analysis test showed that there was a significant effect of ACN on triglyceride concentrations, total cholesterol, LDL-C and HDL-C concentrations in all subgroups of participants (with vs. without dyslipidemia at baseline) and intervention type (supplement/extract vs. food). However, we did not observe any significant effect on apo A and apo B concentrations.

Conclusions
ACN intake in the forms of natural foods and supplements can induce healthy changes in body fat mass, glycemic and lipidemic status and these effects are more prominent in the subjects with above-normal values. This meta-analysis was registered at http://www.crd.york.ac.uk/Prospero (Registration no. CRD42021286466).
Associations of metabolic syndrome, its severity with cognitive impairment among hemodialysis patients
Authors: Yang Yuqi , Li Qian , Long Yanjun , Yuan Jing , Zha Yan 
Published: May 2023

Abstract
Background
In the general population, metabolic syndrome (MetS) is associated with increased risk of cognitive impairment, including global and specific cognitive domains. These associations are not well studied in patients undergoing hemodialysis and were the focus of the current investigation.

Methods
In this multicenter cross-sectional study, 5492 adult hemodialysis patients (3351 men; mean age: 54.4 ± 15.2 years) treated in twenty-two dialysis centers of Guizhou, China were included. The Mini-Mental State Examination (MMSE) was utilized to assess mild cognitive impairment (MCI). MetS was diagnosed with abdominal obesity, hypertension, hyperglycemia, and dyslipidemia. Multivariate logistic and linear regression models were used to examine the associations of MetS, its components, and metabolic scores with the risk of MCI. Restricted cubic spline analyses were performed to explore the dose–response associations.

Results
Hemodialysis patients had a high prevalence of MetS (62.3%) and MCI (34.3%). MetS was positively associated with MCI risk with adjusted ORs of 1.22 [95% confidence interval (CI) 1.08–1.37, P = 0.001]. Compared to no MetS, adjusted ORs for MCI were 2.03 (95% CI 1.04–3.98) for 22.51 (95% CI 1.28–4.90) for 3, 2.35 (95% CI 1.20–4.62) for 4, and 2.94 (95% CI 1.48–5.84) for 5 components. Metabolic syndrome score, cardiometabolic index, and metabolic syndrome severity score were associated with increased risk of MCI. Further analysis showed that MetS was negatively associated with MMSE score, orientation, registration, recall and language (P < 0.05). Significant interaction effect of sex (P for interaction = 0.012) on the MetS-MCI was observed.

Conclusion
Metabolic syndrome was associated with MCI in hemodialysis patients in a positive dose–response effect.
The northeastern Algeria hydrothermal system: gravimetric data and structural implication
Authors: Bayou Yasser , Abtout Abdeslam , Renaut Rosemary A. , Bouyahiaoui Boualem , Maouche Said , Vatankhah Saeed , Berguig Mohamed Cherif 
Published: May 2023

AbstractThe Tell Atlas of Algeria has a huge potential for hydrothermal energy from over 240 thermal springs with temperatures up to $$98^\circ$$

98
∘

C in the Guelma area. The most exciting region is situated in the northeastern part which is known to have the hottest hydrothermal systems. In this work, we use a high-resolution gravity study to identify the location and origin of the hot water, and how it reaches the surface. Gravimetric data analysis shows the shapes of the anomalies arising due to structures at different subsurface depths. The calculation of the energy spectrum for the data also showcases the depths of the bodies causing anomalies. 3D-Euler deconvolution is applied to estimate the depths of preexisting tectonic structures (faults). These preprocessing steps assist with assessing signal attenuation that impacts the Bouguer anomaly map. The residual anomaly is used in a three-dimensional inversion to provide a subsurface density distribution model that illustrates the locations of the origin of the dominant subsurface thermal systems. Overall, the combination of these standard processing steps applied to the measurements of gravity data at the surface provides new insights about the sources of the hydrothermal systems in the Hammam Debagh and Hammam Ouled Ali regions. Faults that are key to the water infiltrating from depth to the surface are also identified. These represent the pathway of the hot water in the study area.
Magma ascent and degassing processes of the 2011 and 2017–18 eruptions of Shinmoedake in Kirishima volcano group, Japan, based on petrological characteristics and volatile content of magmas
Authors: Saito Genji , Oikawa Teruki , Ishizuka Osamu 
Published: May 2023

AbstractThe eruption activity of Shinmoedake in the Kirishima volcanic group of Japan resumed in 2017–18, following a quiet period during 2011–17. Subplinian eruptions preceded lava effusion in 2011; however, no subplinian eruption occurred during 2017–18. Petrological studies and melt inclusion analyses were conducted to investigate the ascent and degassing of the magma to understand the cause of the different eruption styles. Chemical analysis of the melt inclusions from the 2011 eruption indicates that mafic magma with high volatile content (6.2 wt% H2O, 0.25–1.4 wt% CO2) ascended into the shallow felsic magma (1.9–3.7 wt% H2O, 0.025–0.048 wt% CO2) at depths of 5–6 km. Calculations indicate that the mafic magmas were of lower density (1717–1835 kg m−3) than the felsic magma (2264–2496 kg m−3) at 125 MPa and that the two magmas were mixed. The 2011 mixed magma with high volatile content (4.0 wt% H2O, 0.14–0.70 wt% CO2) had a bubble volume of approximately 50 vol% at 50 MPa, which is likely to have caused the subplinian eruption. The whole-rock and chemical compositions of the plagioclase, clinopyroxene, and orthopyroxene phenocryst cores from 2018 and 2011 were similar, suggesting that the 2018 magma was a remnant of the 2011 magma. Chemical analyses of the groundmass from 2018 and the MELTS calculation indicate that the magma approached chemical equilibrium during 2011–18. Melt inclusion analyses and volcanic gas observation noted a lower bulk volatile content in the 2018 magma (2.1–3.0 wt% H2O, 0.087–0.10 wt% CO2) than that in the 2011 magma. Comparison of the degassed-magma volumes estimated from the S and Cl contents of the melt inclusions, SO2 flux and volcanic gas composition, and erupted-magma volume indicates that excess degassing has been occurring in the magma due to convection since February 2011, which may have decreased the volatile content of the magma. The relatively low volatile content meant that the 2018 magma could not erupt explosively and lava was instead erupted via effusion.
Graphical Abstract
Solar events and solar wind conditions associated with intense geomagnetic storms
Authors: Watari Shinichi , Nakamizo Aoi , Ebihara Yusuke 
Published: May 2023

AbstractIntense magnetic storms pose a systemic threat to the electric power grid. In this study we examined the solar/interplanetary causes of such storms, their peak theoretical and observed intensities, and their occurrence frequency. Using coronal mass ejection (CME) and solar wind data, we selected the 18 intense magnetic storms from 1996 to 2021 with disturbance storm time (Dst) index of less than – 200 nT and analyzed solar events and solar wind conditions associated with them. Approximately 83% of the CMEs associated with the storms were full halo type and more than 83% of the flares associated with the storms were located within 30 degrees in longitude of solar central meridian. The integrated dawn-to-dusk electric field in the solar wind (Ey) showed a good correlation with |min. Dst| of the storms and the peak Ey (Eyp) and the peak southward interplanetary magnetic field showed next good correlations with |min. Dst|. We obtained the Eyp of 236 mV/m for |min. Dst| of 2500 nT of the expected upper limit of Earth’s magnetosphere using the empirical equations from the correlations between |min. Dst| and solar wind parameters and showed that this value of Ey is possible according to the past observations. The Eyp of 54 mV/m for the 13 March 1989 storm and that of 165/79 mV/m for the Carrington storm (|min. Dst|= 1760/850 nT) were also obtained. The analysis using the complimentary cumulative distribution function suggested the probabilities of Ey of 100, 200, 250, and 340 mV/m over the next 100 years to be 0.563, 0.110, 0.060 and 0.026, respectively.
Graphical Abstract
Factors influencing the bone mineral density in Duroc boars
Authors: Hu Lingling , Lu Jinxin , Guo Liangliang , Tan Jiajian , Sun Haiqing , Zhou Yuanfei , Wu Yinghui , Wei Hongkui , Jiang Siwen , Peng Jian 
Published: May 2023

Abstract
Background
Leg weakness affects animal welfare and is one of the primary reasons for culling of boars. Low bone mineral density (BMD) is one of the primary factors contributing to leg weakness. Low BMD also appeared to be associated with severe bone pain and has the highest risk of skeletal fragility. Surprisingly, few studies have been performed on the factors influencing BMD in pigs. Therefore, the primary aim of this study was to identify the influencing factors on boar BMD. Herein, the BMD data were determined through the use of ultrasonography from 893 Duroc boars. Logistic regression model was utilized in the analysis of BMD, in which the explanatory variables in the model were lines, ages, body weights, backfat thicknesses and serum mineral element concentrations (Ca, P, Mg, Cu, Fe, Zn, Mn, Se, Pb and Cd).

Results
Results showed that factors significantly influencing BMD included serum Ca, P concentrations, ages and backfat thicknesses (P < 0.05), in which serum Ca concentrations were positively correlated with BMD (P < 0.01), whereas increasing concentrations of serum P decreased BMD (P < 0.01). The serum Ca/P ratio showed significant quadratic effects on BMD (r = 0.28, P < 0.01), and the Ca/P ratio to achieve the best BMD was determined to be 3.7. Furthermore, BMD also changed with age quadratically (r = 0.40, P < 0.01), and reached a peak value around 47 months. Interestingly, a quadratic (r = 0.26, P < 0.01) increase in the BMD was observed as backfat thickness increased, and the inflection point was calculated at around 17 mm.

Conclusion
In conclusion, BMD characteristics of boars could be detected by ultrasonic method, and serum Ca, serum P, age, and backfat thickness contributed to the greatest effect on BMD.
Can infant carrier hip belts of 2-inch, 4-inch, and 6-inch dimensions influence trunk muscle activities during front infant carrying tasks?
Authors: Ojukwu Chidiebele Petronilla , Omeanu Clinton Ikechukwu , Nwosu Ifeoma Blessing , Ede Stephen Sunday , Aiyegbusi Ibifubara Ayoola , Anyaene Chiamaka Chinyere , Ikele Ikenna Theophilus 
Published: May 2023

Abstract

Background
Utilization of infant carriers (ICs) for childcare activities is common and involves varieties of hip belts to offer maximum maternal and infant support. There is a need to establish ergonomic-based guidelines for IC components as a means of improving their supportive roles.

Objective
This study evaluated the effects of infant carrier (IC) hip-belt dimensions on the erector spinae (ES) and multifidus (MF) muscles of healthy adult women during front infant carrying tasks.

Methods
It utilized three hip belts with different width dimensions (2 inch, 4 inch, and 6 inch) attached to the IC during three front infant carrying tasks, respectively. During each 5-min trial, the activities of the right and left components of the ES and MF muscles were simultaneously monitored via surface electromyography (EMG). Asymmetry ratios of the normalized EMG values of the right and left components of each muscle were calculated.

Results
Utilization of different hip-belt dimensions did not elicit significant (p < 0.05) differences in the electrical activities of the back muscles as well as in their asymmetry ratios. However, marginal differences in the normalized EMG values showed that the 4-inch belt elicited the highest muscular activities in three of the four studied muscles.

Conclusion
During simulated front infant carrying tasks, hip-belt dimensions of the ICs did not influence back muscle activities in healthy adult women. Long-term effects of IC hip-belt dimensions on back muscle activities should be evaluated in future studies.