bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heavy metal contamination in retailed food in Bangladesh: a dietary public health risk assessment
Authors: Begum Ruhena , Akter Rahima , Dang-Xuan Sinh , Islam Shariful , Siddiky Nure Alam , Uddin ASM Ashab , Mahmud Asheak , Sarker Md Samun , Grace Delia , Samad Mohammed Abdus , Lindahl Johanna F. 
Published: April 2023

IntroductionContamination with heavy and toxic metals along the food value chain is a public health concern in Bangladesh.MethodsIn this study, 608 fish and chicken samples from traditional and modern retail outlets in urban, peri-urban, and rural areas were collected and analyzed for chromium (Cr), cadmium (Cd), and lead (Pb) contamination, using atomic absorption spectrometry method. The daily intake, target hazard quotient and the target carcinogenic risk (for lead only) as a result of fish and chicken consumption was calculated based on mean results, and by Monte Carlo simulation in @Risk with 100,000 iterations (quantitative risk assessment).ResultsCr and Cd were detected in 80–86% of both chicken meat and fish samples, while Pb positivity found in chicken meat and fish was 54.9 and 23.3%, respectively. The mean concentration (±SD) of Cr, Cd, and Pb in chicken meat were 0.66 ± 0.93, 0.02 ± 0.03, and 0.09 ± 0.10 mg/kg, respectively; and in fish were 0.49 ± 0.62, 0.02 ± 0.03, and 0.06 ± 0.09 mg/kg, respectively. The estimated daily intakes of Cr, Cd, and Pb from chicken and fish were lower than the maximum tolerable daily intake in all studied areas. In addition, the target carcinogenic risk for Pb in chicken was lower than the negligible range, which indicated the risk of cancer due to exposure to Pb through chicken meat and fish consumption was very low.DiscussionThe present study concludes that consumption of chicken meat and fish in Bangladesh, currently at very low levels, is unlikely to constitute a major health risk for humans in respect to these metals. However, continuous market surveillance for heavy metals in food stuff is recommended, especially since consumers may increase their meat intake.
Effectiveness of neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios in predicting the incidence of nausea and vomiting after total knee arthroplasty in patients with hemophilia A
Authors: Feng Denghe , Wang Dong , Gu Changping , Lv Meng , Liu Zaibo , Wang Yuelan 
Published: April 2023

ObjectiveTo investigate the ability of preoperative neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) to predict postoperative nausea and vomiting (PONV) after total knee arthroplasty (TKA).MethodsThe clinical data of 108 male patients with hemophilia A who underwent TKA an our institution were collected and analyzed. Confounding factors were adjusted by propensity score matching. The best cutoffs of the NLR and PLR were determined by the area under the receiver operating characteristic curve (ROC). The predictive ability of these indexes was assessed by measuring the sensitivity, specificity, and positive and negative likelihood ratios.ResultsThere were significant differences in the use of antiemetics (p = 0.036) and the incidence of nausea (p &lt; 0.001) and vomiting (p = 0.006) between the two groups (NLR &lt;2 and ≥2). An increase in preoperative NLR was an independent risk factor for PONV in patients with hemophilia A (p &lt; 0.05). ROC analysis showed that NLR significantly predicted the occurrence of PONV (cutoff value: 2.20, ROC: 0.711, p &lt; 0.001). In turn, the PLR did not strongly predict PONV.ConclusionsThe NLR is an independent risk factor for PONV in patients with hemophilia A and can significantly predict this event. Thus, follow-up monitoring is essential for these patients.
Characteristics and treatment strategies of aggressive angiomyxoma in women: A retrospective review of 87 cases
Authors: Wang Yang , Bu Xiaoling , Liu Yanxia , Xing Yu , Tong Qing 
Published: April 2023

ObjectiveAggressive angiomyxoma (AAM) is a rare kind of soft tissue tumor. The purpose of this study is to summarize the clinical manifestations and treatment strategy of AAM in women.MethodWe searched the case reports on AAM in EMBASE, Web of Science and PubMed, China biomedical database, Wanfang database, VIP database, and China National Knowledge Internet from the start of database construction to November 2022 without any language restrictions in place. Then, the obtained case data were extracted, summarized, and analyzed.ResultA total of 74 articles were retrieved involving 87 cases. The age ranges of onset were 2–67 years. The median age at onset was 34 years. The size of the tumor varied greatly among individuals, and about 65.5% of them were asymptomatic. MRI, ultrasound, and needle biopsy were used for diagnosis. Surgery was the primary mode of treatment, but it was prone to relapse. Gonadotropin-releasing hormone agonist (GnRH-a) might be used to reduce the tumor size before the operation and prevent recurrence after the operation. For patients who are unwilling to receive surgical treatment, GnRH-a alone could be attempted.ConclusionDoctors should consider the possibility of AAM in women with genital tumors. For surgery, it must be ensured that the negative surgical margin is recommended and achieved for preventing recurrence, but we should not ignore the impact of the excessive pursuit for a negative margin on the patient’s reproductive function protection and postoperative recovery. Long-term follow-up is necessary regardless of whether patients receive medical treatment or surgical treatment.
Computed tomographic angular measurements using a bone-centered three-dimensional coordinate system are accurate in a femoral torsional deformity model and precise in clinical canine patients
Authors: Brühschwein Andreas , Schmitz Bronson , Zöllner Martin , Reese Sven , Meyer-Lindenberg Andrea 
Published: April 2023

IntroductionIn small animal orthopedics, angular measurements in the canine femur are often applied in clinical patients with bone deformities and especially in complex and severe cases. Computed tomography (CT) has been shown to be more precise and accurate than two-dimensional radiography, and several methods are described. Measurement techniques evaluated in normal bones must prove accuracy in deformed bones in clinical settings.ObjectivesThe goals of our study were to evaluate the accuracy of canine femoral torsion angle measurements in a femoral torsional deformity model and to test repeatability and reproducibility of canine femoral neck inclination, torsion, and varus angle measurements in CT datasets of dogs applying a CT-based technique using a three-dimensional (3D) bone-centered coordinate system.Materials and methodsFor precision testing, femoral torsion, femoral neck inclination, and femoral varus angles were measured in CT data of 68 canine hind limbs by two operators, and their results were compared. For accuracy testing, a femoral torsional deformity model was preset from 0° to +/−90° with a goniometer and scanned. Torsion angles were measured in the CT data and compared to the preset value.ResultsIn the femoral torsion model, the Bland–Altman plots demonstrated a mean difference of 2.11°, and the Passing–Bablok analysis demonstrated a correlation between goniometer and CT-based measurements. In the clinical CT scans, intra- and interobserver agreement resulted in coefficients of variation for repeated measurements (%) between 1.99 and 8.26 for the femoral torsion, between 0.59 and 4.47 for the femoral neck inclination, and between 1.06 and 5.15 for the femoral varus angles.DiscussionEvaluation of femoral malformations with torsional deformities is the target area of this technique. Further studies are required to assess its value in different types, degrees, and combinations of osseous deformities and to establish normal reference values and guidelines for corrective osteotomies.ConclusionBased on the results of this study, the accuracy of the torsion angle measurements and the precision of inclination, torsion, and the varus angle measurements were considered acceptable for clinical application.
Ganoderma lingzhi culture enhance growth performance via improvement of antioxidant activity and gut probiotic proliferation in Sanhuang broilers
Authors: Liu Xuzhou , Huang Liling , Shi Yan , Wang Xiaoguo , Luo Yanglan , Wei Shiyan , Qin Yanchun , Lu Yuwen , Zhang Wenlong , Ju Ying , Yan Yong , Liao Yuying 
Published: April 2023

IntroductionThe experiment was conducted to evaluate the effects of Ganoderma lingzhi culture (GLC) as a fermented feed on growth performance, serum biochemical profile, meat quality, and intestinal morphology and microbiota in Sanhuang broilers. In addition, the association between gut bacteria and metabolites was investigated via untargeted metabolomic analysis.MethodsA total of 192 Sanhuang broilers (112 days old) with an initial body weight of 1.62 ± 0.19 kg were randomly allocated to four treatments, six replicate pens per treatment with 8 broilers per pen. The four treatments contain a control diet (corn-soybean meal basal diet, CON), a positive control diet (basal diet + 75 mg/kg chlortetracycline, PCON), and the experimental diets supplemented with 1.5 and 3% of GLC, respectively. The trial includes phase 1 (day 1–28) and phase 2 (day 29–56).ResultsThe results showed that broilers in PCON and GLC-added treatments showed a lower FCR (P &lt; 0.05) in phase 2 and overall period and a higher ADG (P &lt; 0.05) in phase 2. On day 56, the concentrations of serum SOD (P &lt; 0.05), and HDL (P &lt; 0.05) and cecal SCFA contents (P &lt; 0.05) were increased in broilers fed GLC diets. Broilers fed GLC also showed a higher microbiota diversity and an elevated abundance of SCFA-related bacteria in the caecum. The association between intestinal bacteria and metabolites was investigated via correlation analysis. The differential metabolites in the caecum, such as L-beta-aspartyl-L-aspartic acid and nicotinamide riboside, were identified.ConclusionIn summary, dietary GCL supplementation could increase growth performance to some extent. Moreover, GLC might benefit broilers' health by improving serum HDL content, antioxidant status, SCFAs contents, bacterial diversity, and probiotic proliferation in the caecum.
Tumor PD-L1 expression and molecular profiling are not associated with immune checkpoint inhibitor-induced thyroid dysfunction in advanced NSCLC patients
Authors: Horesh Adi , Pollack Rena , Nechushtan Hovav , Dresner-Pollak Rivka , Neuman Tzahi 
Published: April 2023

Background: Immune-checkpoint inhibitors (ICIs) have revolutionized the treatment of advanced non-small cell lung cancer (NSCLC), however are frequently associated with thyroid immune-related adverse events (IRAEs). We investigated the association between patient characteristics, tumor PD-L1 expression and molecular profile with the development of thyroid IRAEs in NSCLC patients.Methods: Single center, retrospective study including 107 NSCLC patients treated with PD-1/PD-L1 inhibitors from April 2016 to July 2020. All patients were euthyroid at baseline with at least two TSH measurements post-treatment initiation. The primary outcome was the difference in tumor PD-L1 expression in patients who developed any thyroid IRAEs versus those who remained euthyroid. Additional outcomes included development of overt thyroid dysfunction, the association of specific molecular alterations with thyroid IRAEs, and onset of thyroid IRAEs as a function of tumor PD-L1 expression.Results: Overall, 37 (34.6%) patients developed any thyroid dysfunction and 18 (16.8%) developed overt thyroid dysfunction. Tumor PD-L1 staining intensity was not associated with thyroid IRAEs. TP53 mutation was less likely to be associated with any thyroid dysfunction (p &lt; 0.05) and no association was found between EGFR, ROS, ALK or KRAS mutations. There was no association between PD-L1 expression and time to develop thyroid IRAEs.Conclusion: PD-L1 expression is not associated with the development of thyroid dysfunction in advanced NSCLC patients treated with ICIs, suggesting that thyroid IRAEs are unrelated to tumor PD-L1 expression.
Bacillus subtilis YtpP and Thioredoxin A Are New Players in the Coenzyme-A-Mediated Defense Mechanism against Cellular Stress
Authors: Tossounian Maria-Armineh , Baczynska Maria , Dalton William , Peak-Chew Sew Yeu , Undzenas Kipras , Korza George , Filonenko Valeriy , Skehel Mark , Setlow Peter , Gout Ivan 
Published: April 2023

Coenzyme A (CoA) is an important cellular metabolite that is critical for metabolic processes and the regulation of gene expression. Recent discovery of the antioxidant function of CoA has highlighted its protective role that leads to the formation of a mixed disulfide bond with protein cysteines, which is termed protein CoAlation. To date, more than 2000 CoAlated bacterial and mammalian proteins have been identified in cellular responses to oxidative stress, with the majority being involved in metabolic pathways (60%). Studies have shown that protein CoAlation is a widespread post-translational modification which modulates the activity and conformation of the modified proteins. The induction of protein CoAlation by oxidative stress was found to be rapidly reversed after the removal of oxidizing agents from the medium of cultured cells. In this study, we developed an enzyme-linked immunosorbent assay (ELISA)-based deCoAlation assay to detect deCoAlation activity from Bacillus subtilis and Bacillus megaterium lysates. We then used a combination of ELISA-based assay and purification strategies to show that deCoAlation is an enzyme-driven mechanism. Using mass-spectrometry and deCoAlation assays, we identified B. subtilis YtpP (thioredoxin-like protein) and thioredoxin A (TrxA) as enzymes that can remove CoA from different substrates. With mutagenesis studies, we identified YtpP and TrxA catalytic cysteine residues and proposed a possible deCoAlation mechanism for CoAlated methionine sulfoxide reducatse A (MsrA) and peroxiredoxin 5 (PRDX5) proteins, which results in the release of both CoA and the reduced form of MsrA or PRDX5. Overall, this paper reveals the deCoAlation activity of YtpP and TrxA and opens doors to future studies on the CoA-mediated redox regulation of CoAlated proteins under various cellular stress conditions.
Antibacterial and Cytotoxic Silica-Polycaprolactone-Chlorogenic Acid Hybrids by Sol–Gel Route
Authors: Catauro Michelina , D’Angelo Antonio , Viola Veronica , Cimmino Giovanna , Pacifico Severina 
Published: April 2023

Organic–inorganic hybrid materials were synthesized by a sol–gel route, using silicon alkoxide together with low molecular weight polycaprolactone and caffetannic acid. The synthesized hybrids were characterized by scanning Fourier-transform infrared (FTIR) spectroscopy, and their surface morphology was acquired by scanning electron microscopy (SEM) analysis. The hybrids were investigated for their antiradical capacity using the DPPH and ABTS tests, while the Kirby–Bauer test was used to evaluate their effects on the growth of Escherichia coli and Enterococcus faecalis. Furthermore, a biologically active hydroxyapatite layer has been observed to form on the surface of intelligently synthesized materials. The MTT direct test showed that the hybrid materials are biocompatible with NIH-3T3 fibroblast cells, while they were cytotoxic towards colon, prostate, and brain tumor cell lines. These results shed new light on the suitability of the synthesized hybrids in the medical field, thus affording knowledge on the features of the bioactive silica–polycaprolactone–chlorogenic acid hybrids.
Comparisons of Physicochemical Properties, Bacterial Diversities, Isoflavone Profiles and Antioxidant Activities on Household and Commercial doenjang
Authors: Lee Hee Yul , Cho Du Yong , Jung Jea Gack , Kim Min Ju , Jeong Jong Bin , Lee Ji Ho , Lee Ga Young , Jang Mu Yeun , Lee Jin Hwan , Haque Md Azizul , Cho Kye Man 
Published: April 2023

In this study, the physicochemical properties (pH, acidity, salinity, and soluble protein), bacterial diversities, isoflavone contents, and antioxidant activities of doenjang (fermented soy paste), household doenjang (HDJ), and commercial doenjang (CDJ), were assessed and compared. The values of pH 5.14–5.94 and acidity 1.36–3.03%, indicated a similar level in all doenjang. The salinity was high in CDJ at 12.8–14.6%, and the protein contents (25.69–37.54 mg/g) were generally high in HDJ. Forty-three species were identified from the HDJ and CDJ. The main species were verified to be Bacillus amyloliquefaciens (B. amyloliquefaciens), B. amyloliquefaciens subsp. plantarum, Bacillus licheniformis, Bacillus sp. and Bacillus subtilis. Comparing the ratios of isoflavone types, the HDJ has an aglycone ratio of >80%, and 3HDJ indicates a ratio of isoflavone to aglycone of 100%. In the CDJ, except 4CDJ, glycosides account for a high proportion of more than 50%. The results of antioxidant activities and DNA protection effects were variedly confirmed regardless of HDJs and CDJs. Through these results, it is judged that HDJs have a variety of bacterial species compared to CDJs, and these are biologically active and converted from glycoside to aglycone. Bacterial distribution and isoflavone contents could be used as basic data.
Urinary Metabolomic Profile of Youth at Risk of Chronic Kidney Disease in Nicaragua
Authors: Hall Samantha M. , Raines Nathan H. , Ramirez-Rubio Oriana , Amador Juan José , López-Pilarte Damaris , O'Callaghan-Gordo Cristina , Gil-Redondo Rubén , Embade Nieves , Millet Oscar , Peng Xiaojing , Vences Selene , Keogh Sinead A. , Delgado Iris S. , Friedman David J. , Brooks Daniel R. , Leibler Jessica H. 
Published: April 2023

Key Points

Urinary concentrations of glycine, a molecule associated with thermoregulation, were elevated among youth from a high-risk region for chronic kidney disease of non-traditional etiology (CKDnt).Urinary concentrations of pyruvate, citric acid, and inosine were lower among youth at higher risk of CKDnt, suggesting renal stress.Metabolomic analyses may shed light on early disease processes or profiles or risk in the context of CKDnt.

Background
CKD of a nontraditional etiology (CKDnt) is responsible for high mortality in Central America, although its causes remain unclear. Evidence of kidney dysfunction has been observed among youth, suggesting that early kidney damage contributing to CKDnt may initiate in childhood.

Methods
Urine specimens of young Nicaraguan participants 12–23 years without CKDnt (n=136) were analyzed by proton nuclear magnetic resonance spectroscopy for 50 metabolites associated with kidney dysfunction. Urinary metabolite levels were compared by, regional CKDnt prevalence, sex, age, and family history of CKDnt using supervised statistical methods and pathway analysis in MetaboAnalyst. Magnitude of associations and changes over time were assessed through multivariable linear regression.

Results
In adjusted analyses, glycine concentrations were higher among youth from high-risk regions (β=0.82, [95% confidence interval, 0.16 to 1.85]; P = 0.01). Pyruvate concentrations were lower among youth with low eGFR (β= −0.36 [95% confidence interval, −0.57 to −0.04]; P = 0.03), and concentrations of other citric acid cycle metabolites differed by key risk factors. Over four years, participants with low eGFR experienced greater declines in 1-methylnicotinamide and 2-oxoglutarate and greater increases in citrate and guanidinoacetate concentrations.

Conclusion
Urinary concentration of glycine, a molecule associated with thermoregulation and kidney function preservation, was higher among youth in high-risk CKDnt regions, suggestive of greater heat exposure or renal stress. Lower pyruvate concentrations were associated with low eGFR, and citric acid cycle metabolites, such as pyruvate, likely relate to mitochondrial respiration rates in the kidneys. Participants with low eGFR experienced longitudinal declines in concentrations of 1-methylnicotinamide, an anti-inflammatory metabolite associated with anti-fibrosis in tubule cells. These findings merit further consideration in research on the origins of CKDnt.
Методика формирования навыков использования элементов здорового образа жизни у учащихся младшего школьного возраста
Authors: Kudryavtsev Mikhail Dmitrievich , Kuzmin Vladimir Andreevich , Tolstopyatov Igor Anatolievich 
Published: April 2023

Решение вопросов сохранения и укрепления здоровья школьников требует проведения новых исследований. Физкультурное образовательное пространство может быть успешно использовано как среда реабилитации и социальной адаптации учащихся [2, 4]. Совершенствование оздоровительного компонента при преподавании физической культуры в начальной школе выдвигает необходимость методологического и теоретического обоснования педагогической физкультурной поддержки учащихся в физкультурном оздоровительном пространстве [3, 5].
PENGEMBANGAN APLIKASI MARKETPLACE UNTUK USAHA MIKRO KECIL MENENGAH (UMKM) BERBASIS ANDROID
Authors: Frinando Rian , Artaye Ketut 
Published: April 2023

Usaha Mikro dan Kecil Menengah (UMKM) saat ini sangat berkembang pesat mengikuti perkembangan jaman, memiliki wawasan teknologi yang up-to-date, dan jeli membaca peluang serta berinovasi untuk mengembangkan usahanya. Pertumbuhan UMKM yang signifikan akan menghasilkan profit yang terus meningkat. Seiring dengan semakin ketatnya persaingan dalam dunia bisnis, oleh karenanya UMKM juga dituntut untuk menerapkan strategi jitu terutama dalam pemasaran dan penjualan produknya. Teknologi internet yang berkembang sangat meluas dan memasyarakat dirasakan sangat dibutuhkan di berbagai bidang usaha, hal tersebut menimbukan terciptanya suatu pasar elektronik yang kita kenal dengan sebutan marketplace.

Melalui metode prototyping perangkat lunak yang digunakan dapat merancang marketplace yang sangat menguntungkan para pelaku usaha, terutama usaha mikro, kecil dan menengah. Dengan marketplace setiap pelaku usaha terbantukan yaitu dapat menampilkan produk yang djual tanpa perlu repot membangun sistem. Marketplace mempermudah pelaku usaha mikro, kecil dan menengah tersebut dalam melakukan operasional. Pasar virtual ini maka para pelaku usaha hanya perlu memberikan informasi selengkap - lengkap nya tentang produk yang mereka jual di marketplace seperti informasi produk, harga, pengiriman dan lain – lainnya. Tujuan dari penelitian ini adalah merancang aplikasi marketplace UMKM berbasis android, agar dapat digunakan sebagai wadah untuk melakukan transaksi pemasaran dan pembelian produk yang dihasilkan oleh setiap pelaku UMKM. Marketplace UMKM ini difasilitasi aplikasi menggunakan android studio, dan database.