bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paeonol enhances macrophage phagocytic function by modulating lipid metabolism through the P53-TREM2 axis
Authors: Miao Jifei , Liu Xiaoming , Liao Yuanpin , Li Yiwen , Kuang Yingyan , Zheng Juanxia , Lan Jiao 
Published: May 2023

Abstract
Background The emerging concept of immunometabolism highlights the interplay between lipid metabolism and phagocytosis in macrophages. Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) has been identified as an important modulator of both lipid metabolism and phagocytic function in macrophages. This study aims to investigate the roles of P53 and TREM2 in regulating macrophage lipid metabolism and phagocytosis and to evaluate the potential therapeutic effects of Paeonol on these processes.Methods CRISPR-Cas9 was utilized to generate P53 and TREM2 knockout RAW264.7 cell lines. The dual-luciferase reporter gene assay was performed to assess the interaction between P53 and the TREM2 promoter. A series of functional assays were conducted to evaluate the impact of P53 and TREM2 on macrophage lipid metabolism and phagocytic function. The effects of Paeonol on these processes were also examined.Results Our findings revealed that P53 acts as a transcription factor that upregulates the expression of TREM2, promoting macrophage lipid metabolism, metabolic activity, and phagocytic capacity. Paeonol treatment significantly enhanced the phagocytic function of macrophages. Additionally, the interaction between P53 and the TREM2 promoter was confirmed through dual-luciferase reporter gene assays.Conclusions This study provides novel insights into the roles of P53 and TREM2 in regulating macrophage lipid metabolism and phagocytic function. Furthermore, our findings suggest that Paeonol could be a promising therapeutic agent for modulating macrophage function in various diseases. Further research is warranted to explore the potential applications of Paeonol and to elucidate the molecular mechanisms underlying the observed effects.
Dynamic Topic Modelling for Exploring the Scientific Literature on Coronavirus: An Unsupervised Labelling Technique
Authors: Guillén-Pacho Ibai , Badenes-Olmedo Carlos , Corcho Oscar 
Published: May 2023

Abstract
Purpose: The work presented in this article focusses on improving the interpretability of probabilistic topic models created from a large collection of scientific documents that evolve over time.
Methods: Several time-dependent approaches based on topic models were compared to analyse the annual evolution of latent concepts in the CORD-19 corpus: Dynamic Topic Model, Dynamic Embedded Topic Model, and BERTopic. Then COVID-19 period (December 2019 - present) has been analysed in greater depth, month by month, to explore the evolution of what is written about the disease.
Results: The evaluations suggest that the Dynamic Topic Model is the best choice to analyse the CORD-19 corpus. A novel topic labelling strategy is proposed for dynamic topic models to analyse the evolution of latent concepts. It incorporates content changes in both the annual evolution of the corpus and the monthly evolution of the COVID-19 disease. The generated labels are manually validated using two approaches: through the most relevant documents on the topic, and through the documents that share the most semantically similar label topics.
Conclusions: The labelling enables the interpretation of topics. The novel method for dynamic topic labelling fits the content of each topic and supports the semantics of the topics.
From a trickle to a deluge of Spent EV batteries: Are we prepared?
Authors: Kumar Amit , Huyn Pierre , Vennelakanti Ravigopal 
Published: May 2023

Abstract
The rapid growth in global electric vehicle sales over the past decade has led to unsustainable and unethical extraction of natural resources, broken supply chains for cathodes, and a likelihood of significant environmental hazards from a deluge of spent batteries expected in coming years. Even though it is now technologically feasible to give the spent batteries a second life or harvest critical raw materials, significant challenges remain in tracking spent batteries, optimizing reverse logistics costs, and maximizing value recovery. We propose an ecosystem value optimization approach powered by a digital solution framework to enable five key value drivers for battery circularity – safety, regulatory compliance, carbon footprint reduction, quality, and financials. For example, stakeholders can cut average transportation costs of spent batteries by 11–44% compared to current shipping practices; and improve value recovery by 52–60% by routing batteries with good health to second-life application providers.
Effectiveness of thyroid hormones transporters and deiodinases in skeletal muscle of aging mice
Authors: Zou Yuxin , Wei Lijun , Qi Xinyu , Fang Jia , Zhang Daowen , Shi Runqing , Liu Wei , Fu Yu , Sheng Yunlu , Lv Yifan , Xia Fan , Ding Guoxian , Duan Yu 
Published: May 2023

Abstract
Background and aim Skeletal muscle (SM) has been shown as a target of thyroid hormones (THs). However, the status and effectiveness of thyroid hormones transporters (THTs) and deiodinases in thyroid hormones signaling system (TH signaling) during aging are uncertain. This study aimed to explore the regulated relationships of TH signaling elements in SM of aging mice.Methods Twenty-four C57BL/6J male mice were divided into 6-, 15- and 24-month (6, 15 and 24M) groups according to different ages. The types and expression of THTs and deiodinases were analyzed by mRNA sequencing and proteomic sequencing. qRT-PCR, Western blotting and fluorescence immunoassay were used to compare the key factors of TH signaling in three groups. The ability of transported THs in THTs was analyzed by [125I] T3 and [125I] T4 uptake rate. The regulatory relationship of TH signaling was analyzed in C2C12 cells.Results We identified eight types of THTs in SM of mice, among which the expression of MCT8, MCT10, LAT2, LAT4 and OATP2B1 increased with age. MCT8, MCT10, LAT2 and OATP2B1 had the ability to take up [125I] T3, while MCT8, MCT10 and OATP2B1 possessed the ability to take up [125I] T4 in C2C12 cells. Expression levels of deiodinase type 2 (DIO2) and type 3 (DIO3) also augmented with aging, and the upregulation of DIO3 was regulated by MCT8 and MCT10. Neither deiodinases nor age-related THTs was regulated by TRα.Conclusions The expression of main types of THTs (MCT8, MCT10, LAT2, LAT4 and OATP2B1) and deiodinases (DIO2, DIO3) significantly increased in SM of aged mice, perhaps due to the compensation of age-related decrease of TRα. The expression of DIO3 was regulated by MCT8 and MCT10.
A service provider's experience providing telehealth services during the COVID-19 pandemic in a community-based opioid substitution clinic in Bangladesh
Authors: SHAFIQ TANVEER KHAN IBNE , Rahman Md. Ferdous , Shaikh Kamruzzaman , Sarwar Golam , Hemel Muhammad Manwar Morshed , Khan Tasdik Hyet , Chowdhury Sujan , Khan Sharful Islam 
Published: May 2023

Abstract
Introduction: The COVID-19 pandemic incurred numerous impediments on day-to-day emergency medical services including Opioid Substitution Therapy (OST) for People Who Inject Drugs (PWID). To prevent treatment cessation and lost to follow-up, we tried to implement an alternate mitigating intervention like telemedicine.Methodology: This research was conducted on a cohort of OST clients during the COVID-19 pandemic (from 1st April 2020 to 31st March 2021, trial registration number 00928, dated 06.08.2018) in Narayanganj, a port city adjacent to the capital Dhaka and one of the most COVID-affected districts, with a high PWID and HIV burden. The participants were male, female and transgender women who were all ex-PWID and were under OST services. A telehealth intervention model was designed and implemented in the OST clinic at Narayangonj. Quantitative and qualitative data were collected during pre and post-intervention of telehealth services.Results A total of 297 OST clients of Narayangonj were provided with telehealth services from April 2020 to March 2021. The participants were predominantly male (98.7%), 37.7% were between 30–39 years of age. 39.4% of the telephone calls were related to COVID-19-related symptoms followed by 21.7% for opioid withdrawal, 12.5% for COVID-19 & vaccine-related information, 11.3% for chronic diseases like diabetes, hypertension and asthma, 9.3% for SSTI, and 5.8% for methadone-related effects. There was an improvement in treatment retention (29–87%), loss to follow-up (20–8%), and overdose-related death (1.3–0%) from pre to post intervention of telehealth services.Conclusion From our experience, we found that the telehealth intervention is beneficial for the OST clients and thus ensures treatment continuity and retention, both of which serve as crucial success indicators of the OST programme. Using simply the mobile phone, this intervention can reduce structural and logistical needs like clinic spaces and fewer human resources, ensuring cost-effectiveness and value for money.
Origin and stability of the charge density wave in ScV6Sn6
Authors: Musfeldt Janice , Gu Yanhong 
Published: May 2023

Abstract
Kagom′e metals are widely recognized as versatile platforms for exploring novel topological properties, unconventional electronic correlations, magnetic frustration, and superconductivity. In the RV6Sn6 family of materials (R = Sc, Y, Lu), ScV6Sn6
hosts an unusual charge density wave ground state as well as structural similarities with the AV3Sb5 system (A = K, Cs, Rb). In this work, we combine Raman scattering spectroscopy with first-principles lattice dynamics calculations to reveal the charge density wave state in ScV6Sn6. In the low temperature phase, we find a five-fold splitting of the V-containing totally symmetric mode near 240 cm-1 suggesting that the density wave acts to mix modes of P6/mmm and R-3m symmetry - an effect that we quantify by projecting phonons of the high symmetry state onto those of the lower symmetry structure. We also test the stability of the density wave state under compression and find that both physical and chemical pressure act to quench the effect. We discuss these findings in terms of symmetry and the structure-property trends that can be unraveled in this system.
in vivo mapping of hemodynamic responses mediated by tubuloglomerular feedback in healthy and hypertensive rat kidneys
Authors: Lee Blaire S , Postnov Dmitry D , Sørensen Charlotte M , Sosnovtseva Olga 
Published: May 2023

Abstract
The kidney has a sophisticated vascular structure that performs the unique function of filtering blood and managing blood pressure. Tubuloglomerular feedback is an intra-nephron negative feedback mechanism stabilizing single-nephron blood flow, glomerular filtration rate, and tubular flow rate, which is exhibited as self-sustained oscillations in single-nephron blood flow. We report the application of multi-scale laser speckle imaging to monitor global blood flow changes across the kidney surface (low zoom) and local changes in individual microvessels (high zoom) in normotensive and spontaneously hypertensive rats \textit{in vivo}. We reveal significant differences in the parameters of TGF-mediated hemodynamics and patterns of synchronization. Furthermore, systemic infusion of a glucagon-like-peptide-1 receptor agonist, a potential renoprotective agent, induces vasodilation in both groups but only alters the magnitude of the TGF in Sprague Dawleys, although the underlying mechanisms remain unclear.
Interface-Driven Spontaneous Differentiation-Repulsion Behavior in Isochemical Droplet Populations
Authors: Deshpande Siddharth , Appelman Niels , Chen Chang , Gruppen Ivar 
Published: May 2023

Abstract
An important exercise for understanding the emergent behavior in biology is to study it using minimal synthetic systems. Current biomimetic systems involving directed motion and collective migration require at least three components: two immiscible solvents to form an emulsion, and external agent(s), usually surfactants, to give rise to asymmetric interactions and induce transient non-equilibrium conditions. Here, we present the most minimal system thinkable, consisting of only two components, in the form of micron-sized oil (1-decanol) droplets dispersed in a finite water reservoir. We report spontaneous emergent dynamics within chemically identical droplet populations, in the form of physical differentiation of droplets in a stochastic manner leading to an immediate repulsive behavior amongst their neighbors. Using microfluidic production platform, fluorescence microscopy experiments, and modelling, we show that this cyclic phenomenon of differentiation-repulsion is a result of oil droplets entering the air-water interface leading to Marangoni flows and their coupling with evaporative flux of decanol. We illustrate the potential of this platform by demonstrating control over the event frequency, its use as Marangoni tweezers to trap droplet clusters, and proof-of-principle reorganization of multi-component assemblies. The presented collective behavior with exceptional chemical simplicity makes it amenable for developing self-assembled biomimetic and bioengineered structures.
Rationally Designed Potent and Selective CK1α Degraders Exert Antiproliferative Activity Against a Broad Range of Human Cancer Cell Lines
Authors: Rankovic Zoran , Nishiguchi Gisele , Abdelhamed Sherif , Young Sarah , Caine Elizabeth , Mascibroda Lauren , Kooijman Jeffrey , Das Sourav , Miller Darcie , McGowan Kevin , Mayasundari Anand , Shi Zhe , Barajas Juan , Hiltenbrand Ryan , Aggarwal Anup , Chang Yunchao , Mishra Vibhor , Kalathur Ravi , Peng Junmin , Pruett-Miller Shondra , Daniels Danette , Urh Marjeta , Shelat Anang , Mullighan Charles , Riching Kristin , Zaman Guido , Fischer Marcus , Klco Jeffery , Narina Shilpa , Loughran Allister , High Anthony 
Published: May 2023

Abstract
While the PROTAC approach to targeted protein degradation greatly benefits from rational design, the discovery of molecular glue degraders currently relies on screening strategies. Here, we describe screening of a library containing 3,630 cereblon binders against a panel of 9 patient-derived cancer cell lines. This led to the discovery of SJ7590, a potent degrader of CK1α, IKZF1 and IKZF3 proteins. Through a structure-guided optimization strategy we developed SJ3149, a uniquely potent and selective CK1α degrader. The crystal structure of the CK1α+CRBN+DDB1+SJ3149 quaternary complex provided a rationale for the improved degradation properties via direct contacts between SJ3149 and CK1α. In a panel of 115 human cancer cell lines SJ3149 displayed broad antiproliferative activity. This activity profile, which showed statistically significant correlation with MDM2 inhibitor Nutlin-3a, suggests potential for the development of treatments for hematological cancers, as well as solid tumors.
Renoprotective effect of Chrysanthemum coronarium L. extract on adenine-induced chronic kidney disease in mice
Authors: Kim Yiseul , Lee Ae Sin , Hur Haeng Jeon , Lee Sang Hee , Na Hyun Jin , Sung Mi Jeong 
Published: May 2023

Abstract
Chronic kidney disease (CKD) is a progressive loss of kidney function associated with inflammation and fibrosis. Chrysanthemum coronarium L. (CC), a leafy vegetable, possesses various beneficial properties, including anti-oxidative, anti-inflammatory, and antiproliferative effects. This study aimed to determine the renoprotective effects of CC on adenine-induced CKD in mice. CKD was induced by feeding mice an adenine diet (0.25% w/w) for 4 weeks. Changes in renal function, histopathology, inflammation, and renal interstitial fibrosis were analyzed. The adenine-fed mice were characterized by increased blood urea nitrogen, serum creatinine, and histological changes, including inflammation and fibrosis; however, these changes were significantly restored on treatment with CC. Additionally, CC inhibited the expression of inflammatory markers, monocyte chemoattractant protein-1, interleukins-6 and − 1β, intercellular adhesion molecule-1, and cyclooxygenase 2. Moreover, CC suppressed the expression of the fibrotic markers, type IV collagen, and fibronectin. Furthermore, CC attenuated the expression of profibrotic genes (tumor growth factor-β and α- smooth muscle actin) in adenine-induced renal injury mice. These results suggest that CC has the potential to attenuate adenine-induced renal injury and might offer a new option as a renoprotective agent or functional food supplement for moderate CKD.
African rice (Oryza glaberrima) genomic introgressions impacting upon panicle architecture in Asian rice (O. sativa) lead to the identification of key QTLs
Authors: Adam Hélène , Gutierrez Andrés , Couderc Marie , Sabot François , Ntakirutimana Fabrice , Serret Julien , Orjuela Julie , Tregear James , Jouannic Stefan , Lorieux Mathias 
Published: May 2023

Abstract
Background: Developing high yielding varieties is a major challenge for breeders tackling the challenges of climate change in agriculture. The panicle (inflorescence) architecture of rice is one of the key components of yield potential and displays high inter- and intra-specific variability. The genus Oryza features two different crop species: Asian rice (Oryza sativa L.) and the African rice (O. glaberrima Steud). One of the main morphological differences between the two independently domesticated species is the structure (or complexity) of the panicle, with O. sativa displaying a highly branched panicle, which in turn produces a larger number of grains than that of O. glaberrima. The genetic interactions that govern the diversity of panicle complexity within and between the two species are still poorly understood.
Results: To identify genetic factors linked to panicle architecture diversity in the two species, we used a set of 60 Chromosome Segment Substitution Lines (CSSLs) issued from third generation backcross (BC3DH) and carrying genomic segments from O. glaberrima cv. MG12 in the genetic background of O. sativa Tropical Japonica cv. Caiapó. Phenotypic data were collected for rachis and primary branch length, primary, secondary and tertiary branch number and spikelet number. A total of 15 QTLs were localized on chromosomes 1, 2, 3, 7, 11 and 12 and QTLs associated with enhanced secondary and tertiary branch numbers were detected in two CSSLs. Furthermore, BC4F3:5 lines carrying different combinations of substituted segments were produced to decipher the effects of the identified QTL regions on variations in panicle architecture. A detailed analysis of phenotypes versus genotypes was carried out between the two parental genomes within these regions in order to understand how O. glaberrima introgression events may lead to alterations in panicle traits.
Conclusion: Our analysis led to the detection of genomic variations between O. sativa cv. Caiapó and O. glaberrima cv. MG12 in regions associated with enhanced panicle traits in specific CSSLs. These regions contain a number of key genes that regulate panicle development in O. sativa and their interspecific genomic variations may explain the phenotypic effects observed.
Trends in recurrence of primary spontaneous pneumothorax in young population after treatment for first episode based on a nationwide population data
Authors: Yi Eunjue , Park Jun Eun , Chung Jae Ho , Ahn Chi Bum , Chung Eugene , Noh O kyu , Lee Sungho 
Published: May 2023

Abstract
The aim of this study is to identifying post treatment recurrence rates in pneumothorax patients under 35 and without any comorbidities according to the treatment types, gender, and age categories based on nationwide population data. Clinical information of pneumothorax patients was extracted from the Korean National Health Insurance Service (NHIS) database between January 2002 and December 2020. Enrolled patients were categorized into two groups; (1) Group I, those who underwent conservative management including pain relief, oxygen therapy, and closed thoracostomy, and (2) Group II, surgical intervention. Recurrence rates were compared according to age, gender, and type of treatment. Surgical intervention was performed in 25.6% patients as first treatment. The overall recurrence rate was 20.3%. Male patients showed a higher 5-year recurrence rate than female (20.8% vs. 10.9%, p < 0.001). Those with conservative management showed lower 5-year recurrence rates than those with surgical treatment (7.9% vs. 23.7%, p < 0.001). The 5-year recurrence rates of patients aged 14≤, and < 20 was higher than other age groups. (29.2% vs. 4.5 and 11.9%, p < 0.001). Surgical intervention, male gender and aged under 20 showed association with higher recurrence rates.
Altered sexual reward processing in patients with lifelong premature ejaculation
Authors: Li Yansong , Wang Zixiang , Chen Xi , Sescousse Guillaume , Santtila Pekka , Dai Yutian , Zhang Bing 
Published: May 2023

Abstract
Given that sexual behavior is usually pleasurable and highly rewarding, it is surprising that there is as yet no known research to empirically assess how premature ejaculation (PE) patients respond to the rewarding aspect of sexual behavior. This study was designed to address this issue by evaluating how these men respond to the anticipation and hedonic experience of sexual rewards in comparison to non-sexual rewards. Thirty lifelong PE patients and thirty healthy controls (HCs) performed the incentive delay task manipulating both erotic and monetary rewards. Compared to HCs, lifelong PE patients exhibited significantly faster RTs to erotic cues than to monetary cues during reward anticipation. Meanwhile, hedonic experience ratings after obtaining the actual reward showed that erotic rewards were rated as more pleasant than monetary rewards only by lifelong PE patients. These findings indicate the existence of dysfunctional reward processing in lifelong PE patients, which is characterized by increased incentive motivation elicited by sexual cues and enhanced hedonic impact of sexual rewards. This study may offer an insightful clue regarding how PE is related to the abnormal regulation of the rewarding aspect of sexual behavior.
Abr, a Rho-regulating protein, modulates osteoclastogenesis by enhancing lamellipodia formation by interacting with poly(ADP-ribose) glycohydrolase
Authors: Farhana Fatima , Sakai Eiko , Koyanagi Yu , Yamaguchi Yu , Alam Mohammad Ibtehaz , Okamoto Kuniaki , Tsukuba Takayuki 
Published: May 2023

Abstract
Background: Osteoclasts are multinucleated bone-resorbing cells formed by the fusion of monocyte/macrophage lineage. During osteoclast differentiation, Rho GTPases are involved in various processes, including cell migration, adhesion, and polarity. However, the role of Rho-regulatory molecules in the regulation of osteoclast differentiation remains unclear. In this study, among these genes, we focused on active breakpoint cluster region-related (Abr) protein that is a multifunctional regulator of Rho GTPases.
Methods and Results: We examined using knockdown and overexpression experiments in RANKL-stimulated RAW-D macrophages whether Abr regulates osteoclast differentiation and cell morphology. We observed an increase in Abr expression during osteoclast differentiation and identified expression of a variant of the Abrgene in osteoclasts. Knockdown of Abrsuppressed osteoclast differentiation and resorption. Abr knockdown markedly inhibited the expression of osteoclast markers, such as Nfatc1, c-fos, Src, and Ctsk in osteoclasts. Conversely, overexpression of Abr enhanced the formation of multinucleated osteoclasts, bone resorption activity, and osteoclast marker gene expression. Moreover, Abr overexpression accelerated lamellipodia formation and induced the formation of well-developed actin in osteoclasts. Importantly, the Abr protein interacted with poly(ADP-ribose) glycohydrolase (PARG) and Rho GTPases, including RhoA, Rac1/2/3, and Cdc42 in osteoclasts.
Conclusions: Taken together, these results indicate that Abr modulates osteoclastogenesis by enhancing lamellipodia formation via its interaction with PARG.