bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periaqueductal gray matter and medial prefrontal cortex reflect negative prediction errors during differential conditioning
Authors: Gorka Adam X , Philips Ryan T , Torrisi Salvatore , Manbeck Adrienne , Goodwin Madeline , Ernst Monique , Grillon Christian 
Published: May 2023

Abstract
Computational models of associative learning posit that negative prediction errors (PEs) arising from the omission of aversive outcomes weaken aversive Pavlovian associations during differential conditioning and extinction. It is possible that negative PEs may underlie exaggerated conditioned responses to the conditioned stimulus not paired with an aversitve outcome (CS-) during differential conditioning and to the conditioned stimulus originally paired with a aversive outcome (CS+) during extinction in patients with clinical anxiety disorders. Although previous research has demonstrated that manipulations of the periaqueductal gray matter (PAG) interfere with extinction learning in animals, the role of the PAG in processing negative PEs within the human brain is presently unclear. We set out to investigate how PAG responses and connectivity are impacted by negative PEs using ultra-high-field (7 T) functional magnetic resonance imaging and hierarchical Bayesian analysis. During differential conditioning, negative PEs were associated with larger responses within the lateral and dorsolateral PAG and increased connectivity between the dorsolateral PAG and medial areas of Brodmann area 9. Collectively, these results shed light on the association between activity within the PAG and medial prefrontal cortex and the omission of aversive outcomes during Pavlovian learning.
Phylogenomic Analyses Reveal an Allopolyploid Origin of Core Didymocarpinae (Gesneriaceae) Followed by Rapid Radiation
Authors: Yang Lihua , Harris A J , Wen Fang , Li Zheng , Feng Chao , Kong Hanghui , Kang Ming 
Published: May 2023

Abstract
Allopolyploid plants have long been regarded as possessing genetic advantages under certain circumstances due to the combined effects of their hybrid origins and duplicated genomes. However, the evolutionary consequences of allopolyploidy in lineage diversification remain to be fully understood. Here, we investigate the evolutionary consequences of allopolyploidy using 138 transcriptomic sequences of Gesneriaceae, including 124 newly sequenced, focusing particularly on the largest subtribe Didymocarpinae. We estimated the phylogeny of Gesneriaceae using concatenated and coalescent-based methods based on five different nuclear matrices and 27 plastid genes, focusing on relationships among major clades. To better understand the evolutionary affinities in this family, we applied a range of approaches to characterize the extent and cause of phylogenetic incongruence. We found that extensive conflicts between nuclear and chloroplast genomes and among nuclear genes were caused by both incomplete lineage sorting (ILS) and reticulation, and we found evidence of widespread ancient hybridization and introgression. Using the most highly supported phylogenomic framework, we revealed multiple bursts of gene duplication throughout the evolutionary history of Gesneriaceae. By incorporating molecular dating and analyses of diversification dynamics, our study shows that an ancient allopolyploidization event occurred around the Oligocene–Miocene boundary, which may have driven the rapid radiation of core Didymocarpinae.
Nutrient conditions mediate mycorrhizal effects on biomass production and cell wall chemistry in poplar
Authors: Becklin Katie M , Viele Bethanie M , Coleman Heather D 
Published: May 2023

Abstract
Large-scale biofuel production from lignocellulosic feedstock is limited by the financial and environmental costs associated with growing and processing lignocellulosic material and the resilience of these plants to environmental stress. Symbiotic associations with arbuscular (AM) and ectomycorrhizal (EM) fungi represent a potential strategy for expanding feedstock production while reducing nutrient inputs. Comparing AM and EM effects on wood production and chemical composition is a necessary step in developing biofuel feedstocks. Here, we assessed the productivity, biomass allocation and secondary cell wall (SCW) composition of greenhouse-grown Populus tremuloidesMichx. inoculated with either AM or EM fungi. Given the long-term goal of reducing nutrient inputs for biofuel production, we further tested the effects of nutrient availability and nitrogen:phosphorus stoichiometry on mycorrhizal responses. Associations with both AM and EM fungi increased plant biomass by 14–74% depending on the nutrient conditions but had minimal effects on SCW composition. Mycorrhizal plants, especially those inoculated with EM fungi, also allocated a greater portion of their biomass to roots, which could be beneficial in the field where plants are likely to experience both water and nutrient stress. Leaf nutrient content was weakly but positively correlated with wood production in mycorrhizal plants. Surprisingly, phosphorus played a larger role in EM plants compared with AM plants. Relative nitrogen and phosphorus availability were correlated with shifts in SCW composition. For AM associations, the benefit of increased wood biomass may be partially offset by increased lignin content, a trait that affects downstream processing of lignocellulosic tissue for biofuels. By comparing AM and EM effects on the productivity and chemical composition of lignocellulosic tissue, this work links broad functional diversity in mycorrhizal associations to key biofuel traits and highlights the importance of considering both biotic and abiotic factors when developing strategies for sustainable biofuel production.
Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma
Authors: Fang Teng , Sun Hao , Sun Xiyue , He Yi , Tang Peixia , Gong Lixin , Yu Zhen , Liu Lanting , Xie Shiyi , Wang Tingyu , Xu Zhenshu , Yi Shuhua , An Gang , Xu Yan , Zhu Guoqing , Qiu Lugui , Hao Mu 
Published: May 2023

Background:
MicroRNAs (MiRNAs) carried by exosomes play pivotal roles in the crosstalk between cell components in the tumor microenvironment. Our study aimed at identifying the expression profile of exosomal miRNAs (exo-miRNAs) in the serum of multiple myeloma (MM) patients and investigating the regulation networks and their potential functions by integrated bioinformatics analysis.

Material and Methods:
Exosomes in serum from 19 newly diagnosed MM patients and 9 healthy donors were isolated and the miRNA profile was investigated by small RNA sequencing. Differential expression of exo-miRNAs was calculated and target genes of miRNAs were predicted. CytoHubba was applied to identify the hub miRNAs and core target genes. The LASSO Cox regression model was used to develop the prognostic model, and the ESTIMATE immune score was calculated to investigate the correlation between the model and immune status in MM patients.

Results:
The top 6 hub differentially expressed serum exo-miRNAs were identified. 513 target genes of the 6 hub exo-miRNAs were confirmed to be differentially expressed in MM cells in the Zhan Myeloma microarray dataset. Functional enrichment analysis indicated that these target genes were mainly involved in mRNA splicing, cellular response to stress, and deubiquitination. Thirteen core exo-miRNA target genes were applied to create a novel prognostic signature to provide risk stratification for MM patients, which is associated with the immune microenvironment of MM patients.

Conclusions:
Our study comprehensively investigated the exo-miRNA profiles in MM patients. A novel prognostic signature was constructed to facilitate the risk stratification of MM patients with distinct outcomes.
Early Postoperative Therapeutic Scleral Lens Intervention for Penetrating Keratoplasty Complications in Atopic Keratoconjunctivitis
Authors: Asghari Bita , Brocks Daniel C. 
Published: May 2023

Abstract
A 52-year-old white man with keratoconus and severe atopic keratoconjunctivitis underwent penetrating keratoplasty (PK) for visual rehabilitation in the left eye. Post-PK complications included a persistent epithelial defect. Therapeutic scleral lens wear was initiated 2 weeks post-PK. Customizations were incorporated into the lens design to prevent suction and corneal hypoxia during lens wear. Post-PK herpes epithelial keratitis developed shortly thereafter, causing recurrence of a nonhealing epithelial defect. The patient was treated with oral antiviral therapy, discontinuation of dupilumab, and daily waking-hour scleral lens wear, which was used as an antibiotic drug delivery device. The cornea fully epithelialized, and best-corrected visual acuity improved to 20/40-2. The patient continued with daily waking-hour scleral lens wear and was without recurrence of persistent epithelial defect or herpes keratitis at 18 months. No scleral lens–related complications were observed despite the unconventional early intervention with a therapeutic scleral lens.
A Pilot Study Examining the Use of Ultrasound to Measure Intravascular Volume Status in Agricultural Workers in a Field-Based Research Setting
Authors: Suarez Jonathan J. , Elon Lisa , Rangel-Rodriguez Adriana , Xiuhtecutli Nezahualcoyotl , Houser Madelyn C. , Hertzberg Vicki , McCauley Linda , Sands Jeff M. , Chicas Roxana C. 
Published: May 2023

Objective
The aim of this pilot study was to explore if internal jugular vein (IJV) ultrasound studies on agricultural workers in a field-based research setting could assess volume status during a hydration intervention.

Methods
We performed pre– and post–work shift IJV ultrasound images on 30 agricultural workers. The IJV collapsibility index values were <39% (euvolemic) or ≥39% (hypovolemic).

Results
Of the water group, 13% (2/15) had an IJV collapsibility index ≥39%, and this increased to 19% (3/16) by the end of the work shifts. The electrolyte group did not have any workers start the work shift with an IJV collapsibility index ≥39%; however, at the postshift assessment, 15% (2/13) were hypovolemic.

Conclusion
Internal jugular vein ultrasounds may have the potential to be a useful tool to determine volume status in field-based research settings. Further investigation is needed to confirm these findings.
Analysis of nurse practitioner practice in US emergency departments: Evidence supporting educational preparation, credentialing, scope of practice, and outcomes
Authors: Wilbeck Jennifer , Davis Wesley D. , Tyler Diane , Schumann Lorna , Kapu April 
Published: May 2023

ABSTRACT
The nurse practitioner (NP) role within emergency care has grown in recent years and now there are an estimated 25,000 NPs employed in diverse emergency care settings. Despite this significant growth and expansion of the NP within emergency care areas, challenges exist. In addition to the pervasive confusion regarding the NP role in emergency care, data and statistics detailing characteristics and outcomes of NP practice in emergency care are either lacking or misrepresented. This article explores barriers and provides current and accurate information describing the current educational preparation, credentialing, scope of practice, and outcomes among NPs in US emergency departments. The totality of available evidence reviewed supports safe, timely, efficient, and patient-centered care provided by NPs in emergency care.
Treatment Course Comparison Between Anxiety-Related Disorders in Adult Outpatients
Authors: Carlier Ingrid V.E. , van der Elst Lotte V. , de Jong Kim , van Vliet Irene M. 
Published: May 2023

AbstractAnxiety-related disorders constitute the leading prevalent mental disorders, with major burden on patients, their relatives, and society. Moreover, there is considerable treatment nonadherence/nonresponse. We used routine outcome monitoring (ROM) data from outpatients covering four anxiety-related disorders (DSM-IV-R,N= 470) to examine their 6-month treatment course and its predictors: generalized anxiety disorder, panic disorder with agoraphobia, obsessive-compulsive disorder, and posttraumatic stress disorder. Measures included Mini-International Neuropsychiatric Interview Plus, Brief Symptom Inventory (BSI), Montgomery-Åsberg Depression Rating Scale (MADRS), Brief Anxiety Scale (BAS), and Short Form Health Survey 36 (SF-36). On the clinician-rated instruments (MADRS/BAS), all anxiety-related disorder groups showed a significant albeit modest improvement after treatment. On the BSI self-rating, only generalized anxiety disorder and posttraumatic stress disorder showed a significant modest improvement. No anxiety-related disorder groups improved significantly regarding SF-36 physical functioning. For BSI symptom course, significant predictors were comorbid somatoform/total disorders, SF-36 physical functioning/general health, and MADRS score. Clinical implications and future research recommendations are discussed.
Relationship Between BMI and Outcomes in Microvascular Abdomen-Based Autologous Breast Reconstruction
Authors: Barnes Laura L. , Lem Melinda , Patterson Anne , Segal Rachel , Holland Michael C. , Lentz Rachel , Sbitany Hani , Piper Merisa 
Published: May 2023

Introduction:
Increasing body mass index (BMI) is a known risk factor for autologous microsurgical breast reconstruction. No prior studies have stratified outcomes across BMI ranges or defined the BMI at which complication rates dramatically increase.

Methods:
We performed a retrospective chart review of all patients who underwent abdomen-based autologous free flap breast reconstruction at our institution between 2004-2021. Clinical, surgical and outcomes data were collected. Patients were stratified into five BMI categories: ≤25, 25.01-30, 30.01-35, 35.01-40 and >40. Complication rates were analyzed across these groups, and a receiver-operating characteristic analysis was used to determine an optimal BMI cutoff point.

Results:
Three hundred and sixty-five patients (545 breasts) were included in this study. The rates of several breast complications significantly increased with increasing BMI at distinct levels, including any breast complication (BMI>30), unplanned re-operation (BMI>35), fat necrosis (BMI>40), wound breakdown requiring re-operation (BMI>35), any infection (BMI>30), infection requiring PO antibiotics (BMI>25), infection requiring IV antibiotics (BMI>35), and mastectomy flap necrosis (BMI>35). The rates of many abdominal complications significantly increased with increasing BMI at distinct levels as well, including delayed wound healing (BMI>30), wound breakdown requiring re-operation (BMI>40), any infection (BMI>25), and infection requiring PO antibiotics (BMI>25). Optimal BMI cutoffs of 32.7 and 30.0 were determined to minimize the occurrence of any breast complication and any abdomen complication, respectively.

Conclusion:
Pre-operative weight loss has great potential to alleviate surgical risk in overweight and obese patients pursuing autologous breast reconstruction. Our results quantify the risk reduction based on a patient’s pre-operative BMI.
68Ga-FAPI PET/CT Imaging of the Pancreas in a Patient With Fulminant Type 1 Diabetes
Authors: Park Seon-Young , Kim Jahae , Chung Jin Ook , Kim Kangbin , Chung Dong Jin 
Published: May 2023

Abstract
Fulminant type 1 diabetes is a recently recognized diabetes subtype characterized by extremely rapid destruction of the pancreatic beta cells, leading to an absolute deficiency in insulin secretion. Fulminant type 1 diabetes is clinically characterized by the drastic onset of hyperglycemia and ketoacidosis within a few days, as well as near-normal glycated hemoglobin (HbA1c) levels despite remarkable hyperglycemia at initial presentation. A 41-year-old woman diagnosed with fulminant type 1 diabetes underwent 68Ga-FAPI PET/CT, which showed intense FAPI uptake throughout the pancreas, especially in the pancreatic tail. Contrast-enhanced abdominal CT failed to reveal any pancreatic abnormalities. This case indicated that 68Ga-FAPI PET/CT might be useful for evaluating patients with fulminant type 1 diabetes.
Pulmonary Sclerosing Pneumocytoma on 18F-FDG PET/MRI
Authors: Hou Wenxiu , Tian Rong 
Published: May 2023

Abstract
Pulmonary sclerosing pneumocytoma is a rare benign neoplasm. Owing to the low incidence, its radiographic features on 18F-FDG PET/MRI are not well-known. Herein, we described findings of pulmonary sclerosing pneumocytoma on 18F-FDG PET/MRI in a 52-year-old woman. It showed moderate FDG uptake and hyperintensity signal on both T1WI and T2WI images.
Extraocular Muscle Enlargement in Acromegaly
Authors: McInnis Rachel L. , Rjoob Hani , Ohorodnyk Pavlo , Fraser John. Alexander , Van Uum Stan H. M. , Bursztyn Lulu L. C. D. 
Published: May 2023

Background:
To determine whether acromegaly is associated with increased extraocular muscle (EOM) size at time of presentation.

Methods:
Patients with a new diagnosis of acromegaly in a single tertiary care clinic with a CT scan that adequately delineated the EOMs were included. Control subjects were age- and sex-matched patients with a new diagnosis of nonfunctioning pituitary adenoma. Retrospective chart review was performed to extract baseline clinical and laboratory parameters including growth hormone, insulin-like growth factor 1, thyroid stimulating hormone, free T3, and free T4. A single neuroradiologist analyzed all CT scans and measured the maximum diameter and cross-sectional area of the superior rectus, inferior rectus, medial rectus, and lateral rectus in both eyes of all patients.

Results:
We evaluated 17 patients with acromegaly and 18 control subjects. Mean maximum diameter of the superior, inferior, medial, and lateral recti were 4.80 mm (SD = 0.81), 4.67 mm (SD = 0.54), 4.86 mm (SD = 0.77), and 4.53 mm (SD = 0.70) respectively, in the acromegaly group. In the control group, they were 3.62 mm (SD = 0.58),3.71 mm (SD = 0.46), 3.66 mm (SD = 0.32), and 3.21 mm (SD = 0.44), respectively. The maximum diameter and cross-sectional area of all 4 EOMs measured in the acromegaly group were significantly larger (P < 0.001) compared with the control group.

Conclusions:
Patients with acromegaly present with significantly enlarged EOMs compared with control subjects with nonfunctioning pituitary adenomas.
Trifunctional linkers enable improved visualization of actin by expansion microscopy
Authors: Wen Gang , Lycas Matthew Domenic , Jia Yuqing , Leen Volker , Sauer Markus , Hofkens Johan 
Published: May 2023

ABSTRACTExpansion Microscopy (ExM) revolutionized the field of super-resolution microscopy by allowing for subdiffraction resolution fluorescence imaging on standard fluorescence microscopes. However, it has been found that it is hard to visualize actin filaments efficiently using ExM. To improve actin imaging, multifunctional molecules have been designed, however, with moderate success. Here, we present optimized methods for phalloidin conjugate grafting that have high efficiency for both cellular and tissue samples. Our optimized strategy improves anchoring and signal retention by ∼10 times. We demonstrate the potential of trifunctional linkers (TRITON) for actin imaging in combination with immunolabeling using different ExM protocols. 10x ExM of actin labeled with TRITON enabled us to visualize the periodicity of actin rings in cultured hippocampal neurons and brain slices by Airyscan confocal microscopy. Thus, TRITON linkers provide an efficient grafting method, especially in cases where the concentration of target-bound monomers is insufficient for high-quality ExM.
Robotic process automation: bibliometric reflection and future opportunities
Authors: Fernandez Dahlia , Dastane Omkar , Omar Zaki Hafizah , Aman Aini 
Published: May 2023

PurposeRobotic Process Automation (RPA) is a digital transformation tool that demonstrated tremendous growth in research output as well as its application in the past decade. This study attempts to identify essential research gaps and proposes future research agendas in the field by analyzing publishing trends, major stakeholders (authors, countries, affiliations, journals), key clusters and evolving themes by mapping the most recent research (2016–2022) in the field.Design/methodology/approachPreferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) was used to retrieve a total of 244 publications from the Web of Science (WOS) database for this analysis. The study then uses the open-source R program bibliometrix to conduct bibliometric analysis. A variety of tools and methods including collaboration network, word dynamics, co-occurrence network, thematic map and strategy map were utilized.FindingsThe analysis reveals the most influential stakeholders (country: the USA, author: Arai K, affiliation: Christ Deemed University), main clusters of intellectual structure (process mining, digital transformation, blockchain, information systems) and the evolution of themes (model innovation, artificial intelligence, big-data, design science and user acceptance) in the subject.Originality/valueThis study uses bibliometric analysis to provide a comprehensive overview of RPA literature which unravels the conceptual structure of the stream and proposes a research agenda for the future. Based on the growth of themes and the strategy map, this study may assist entrepreneurs and practitioners in determining field priorities for strategizing process innovation.
The economic consequence of corporate philanthropic donations: evidence from Jordan
Authors: Ananzeh Husam 
Published: May 2023

PurposeThe purpose of this study is to investigate the impact of Corporate Philanthropic Donations (CPD) on the Corporate Economic Performance (CEP) of a group of Jordanian public shareholding companies.Design/methodology/approachThe sample includes 94 companies listed on the Amman Stock Exchange between 2010 and 2016. Manual content analysis is employed to collect the quantitative-related data needed for this study.FindingsOur findings show that CPD is relevant, with a significant impact on financial performance. More specifically, CPD has a positive impact on the performance indicators measured by Tobin’s Q (TQ), return on equity (ROE) and return on assets (ROA).Research limitations/implicationsThis study contributes to the research debate on CPD and CEP, especially in developing countries. It emphasizes the importance of such practices in increasing corporate profitability.Practical implicationsThe study’s findings highlight the importance of CPD for Jordanian corporate managers. A greater emphasis on donations is likely to attract investor attention, government attention, media attention and humanitarian activism, all of which will enhance corporate goodwill.Originality/valueThis study demonstrates the positive relationship between corporate social responsibility (CSR) and CEP in an emerging economy, with a focus on one aspect of CSR, namely donation, that is underrepresented in developing countries. The study employs multiple methods for analyzing profitability as proxied by TQ, ROE and ROA, given the presence of multiple proxies to measure profitability. A further interesting aspect is examining the topic of CPD in the Jordanian context, where listed companies exhibit a uniform understanding of CPD.