bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electromagnetic field exposure affects the calling song, phonotaxis, and level of biogenic amines in crickets
Authors: Wyszkowska Joanna , Kobak Jarosław , Aonuma Hitoshi 
Published: June 2023

Abstract

Electromagnetic field (EMF) is ubiquitous in the environment, constituting a well-known, but poorly understood stressor. Few studies have been conducted on insect responses to EMF, although they are an excellent experimental model and are of great ecological importance. In our work, we tested the effects of EMF (50 Hz, 7 mT) on the cricketGryllus bimaculatus: the male calling song pattern, female mate choice and levels of biogenic amines in the brain. Exposure of males to EMF modified the number and period of chips in their calling song, but not the sound frequency. Aged (3-weeks-old) females were attracted to both natural and EMF-modified male signals, whereas young (1-week-old, virgin) females responded only to the modified signal, suggesting its higher attractance. A stress response of males to EMF may be responsible for the change in the calling song, as suggested by changes in the amine levels in their brains (an increase in dopamine, tyrosine, and serotonin concentration and a decrease in octopamine level). These findings indicate thatG. bimaculatusresponds to EMF like to stressful conditions, which may change the condition and fitness of exposed individuals, disrupt mate selection and, in consequence, affect the species existence.
Discovery of novel JAK1 inhibitors through combining machine learning, structure-based pharmacophore modeling and bio-evaluation
Authors: Wang Zixiao , Sun Lili , Xu Yu , Liang Peida , Xu Kaiyan , Huang Jing 
Published: June 2023

Abstract
Background
Janus kinase 1 (JAK1) plays a critical role in most cytokine-mediated inflammatory, autoimmune responses and various cancers via the JAK/STAT signaling pathway. Inhibition of JAK1 is therefore an attractive therapeutic strategy for several diseases. Recently, high-performance machine learning techniques have been increasingly applied in virtual screening to develop new kinase inhibitors. Our study aimed to develop a novel layered virtual screening method based on machine learning (ML) and pharmacophore models to identify the potential JAK1 inhibitors.
Methods
Firstly, we constructed a high-quality dataset comprising 3834 JAK1 inhibitors and 12230 decoys, followed by established a series of classification models based on a combination of three molecular descriptors and six ML algorithms. To further screen potential compounds, we constructed several pharmacophore models based on Hiphop and receptor-ligand algorithms. We then used molecular docking to filter the recognized compounds. Finally, the binding stability and enzyme inhibition activity of the identified compounds were assessed by molecular dynamics (MD) simulations and in vitro enzyme activity tests.
Results
The best performance ML model DNN-ECFP4 and two pharmacophore models Hiphop3 and 6TPF 08 were utilized to screen the ZINC database. A total of 13 potentially active compounds were screened and the MD results demonstrated that all of the above molecules could bind with JAK1 stably in dynamic conditions. Among the shortlisted compounds, the four purchasable compounds demonstrated significant kinase inhibition activity, with Z-10 being the most active (IC50 = 194.9 nM).
Conclusion
The current study provides an efficient and accurate integrated model. The hit compounds were promising candidates for the further development of novel JAK1 inhibitors.
Adaptive designs were primarily used but inadequately reported in early phase drug trials
Authors: Wang Yuning , Yao Minghong , Liu Jiali , Liu Yanmei , Ma Yu , Luo Xiaochao , Mei Fan , Xiang Hunong , Zou Kang , Li Ling , Sun Xin 
Published: June 2023

Abstract
Background
Faced with the high costs and low success rates of clinical trials, researchers are increasingly applying adaptive designs to speed up the development of new drugs. However, the application of adaptive design to drug randomized controlled trials (RCTs) and whether the reporting is adequate are unclear. Thus, this study aimed to summarize the epidemiological characteristics of the relevant trials and assess their reporting quality by the Adaptive designs CONSORT Extension (ACE) checklist.
Methods
We searched MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL) and ClinicalTrials.gov from inception to January 2020. We included drug RCTs that explicitly claimed to be adaptive trials or used any type of adaptative design. We extracted the epidemiological characteristics of included studies to summarize their adaptive design application. We assessed the reporting quality of the trials by Adaptive designs CONSORT Extension (ACE) checklist. Univariable and multivariable linear regression models were used to the association of four prespecified factors with the quality of reporting.
Results
Our survey included 108 adaptive trials. We found that adaptive design has been increasingly applied over the years, and was commonly used in phase II trials (n = 45, 41.7%). The primary reasons for using adaptive design were to speed the trial and facilitate decision-making (n = 24, 22.2%), maximize the benefit of participants (n = 21, 19.4%), and reduce the total sample size (n = 15, 13.9%). Group sequential design (n = 63, 58.3%) was the most frequently applied method, followed by adaptive randomization design (n = 26, 24.1%), and adaptive dose-finding design (n = 24, 22.2%). The proportion of adherence to the ACE checklist of 26 topics ranged from 7.4–99.1%, with eight topics being adequately reported (i.e., level of adherence ≥ 80%), and eight others being poorly reported (i.e., level of adherence ≤ 30%). In addition, among the seven items specific for adaptive trials, three were poorly reported: accessibility to statistical analysis plan (n = 8, 7.4%), measures for confidentiality (n = 14, 13.0%), and assessments of similarity between interim stages (n = 25, 23.1%). The mean score of the ACE checklist was 13.9 (standard deviation [SD], 3.5) out of 26. According to our multivariable regression analysis, the trials published more recently (estimated β = 0.14, P < 0.01) and the multicenter trials (estimated β = 2.22, P < 0.01) were associated with better reporting.
Conclusion
Adaptive design has shown an increasing use over the years, and was primarily applied to early phase drug trials. However, the reporting quality of adaptive trials is suboptimal, and substantial efforts are needed to improve the reporting.
Adipocyte PI3K links adipostasis with basal insulin secretion through an adipoincretin effect
Authors: Becattini Barbara , Molinaro Angela , Henricsson Marcus , Boren Jan , Solinas Giovanni 
Published: June 2023

Abstract
Inhibitors of insulin-PI3K signaling potently induce insulin secretion in-vivo, demonstrating that insulin secretion is governed by feedback control. Resolving the mechanism of this feedback is necessary to understand hyperinsulinemia and insulin resistance and to develop optimal PI3K-targeted therapies. Adipose tissue-specific knockout mice for the insulin receptor, or AKT1 and AKT2, are severely lipodystrophic. Thereby, the role of adipocyte insulin signaling in the feedback control of insulin secretion remains unknown. To investigate insulin-PI3K signaling in the adipocyte in vivo, we generated adipocyte-specific PI3Ka knockout mice (PI3KaAdQ). PI3KaAdQ mice and PI3KaF/F control mice showed similar adiposity, indicating compensation from another PI3K activity. PI3Kb-selective inhibitors dumped AKT phosphorylation, specifically in the adipocytes of PI3KaAdQ mice. The PI3Kb-selective inhibitor GSK2636771 markedly increased serum FFA and insulin secretion in PI3KaAdQ mice but not in PI3KaF/F mice, demonstrating that insulin secretion is governed by adipocyte PI3K, a phenomenon that we name the adipoincretin effect. The adipoincretin effect can be induced in mice fasted overnight with decreasing glycemia. The effects of adipocyte-specific PI3K inhibition on insulin secretion and serum FFA could be partly dissociated by cotreating PI3KaAdQ mice with GSK2636771 and the lipolysis inhibitor nicotinic acid. The adipoincretin effect was associated with reduced plasma branched-chain amino acids, reduced serum leptin, and increased 3-hydroxybutyrylcarnitine. These results demonstrate that baseline insulin secretion and lipolysis are coregulated by adipocyte PI3K signaling to control adipostasis during fasting through an adipoincretin effect.
Entomological longitudinal surveys in two contrasted eco-climatic settings in Cameroon reveal a high malaria transmission from Anopheles funestus associated with GSTe2 metabolic resistance
Authors: F. Brice Natchema S. , Tchouakui Magellan , Menze Benjamin D. , Mugenzi Leon M. J. , Fofie Derrick , Nguifo-Nguete Daniel , Nkengazong Lucia , Tombi Jeannette , Wondji Charles S. 
Published: June 2023

Abstract
Background
The impact of metabolic resistance to insecticide on malaria transmission remains poorly characterised notably through application of entomological parameters. The lack of resistance markers has been one of the limiting factors preventing robust assessment of such impact. To this end, the present study sought to investigate how L119F-Gste2 metabolic gene influences entomological parameters underpinning mosquitos’ propensity to transmit Plasmodium species.
Methods
Longitudinal studies were carried out in Mibellon and Elende, two different eco-climatic settings in Cameroon and mosquitoes were collected using Human Landing Catch (HLC), Centre for Disease Control Light Trap (CDC-LT) and Pyrethrum Spray Catch (PSC) technics. Plasmodium sporozoite parasites were detected by TaqMan and Nested PCR, and blood meal origin with ELISA. The allele-specific PCR (AS-PCR) method was used to genotype the L119F-GSTe2 marker and association with malaria transmission was established by comparing key transmission parameters such as the Entomologic Inoculation Rate (EIR) between individuals with different L119F-GSTe2 genotypes.
Results
An. funestus s.l was the predominant malaria vector collected during the entomological survey in both sites (86.6% and 96.4% in Elende and Mibellon respectively) followed by An. gambiae s.l (7.5% and 2.4%). Sporozoite infection rates were very high in both collection sites (8.7% and 11% respectively in Elende and Mibellon). An. funestus s.s exhibited a very high entomological inoculation rate (EIR) (66 ib/h/month and 792 ib/h/year) and was responsible for 98.6% of all malaria transmission events occurring in both sites. Human Blood Index was also high in both locations (HBI = 94%). An. funestus s.s. mosquitoes with both 119F/F (RR) and L119F (RS) genotypes had a significantly higher transmission intensity than their susceptible L/L119 (SS) counterparts (IRR = 2.2, 95%CI (1.1–5.2), p = 0.03; IRR = 2.5, 95% CI (1.2–5.8), p = 0.01 respectively).
Conclusion
This study highlights the major role that An. funestus s.s plays in malaria transmission in Cameroon with an aggravation from GSTe2-based metabolic resistance.
Shear mechanical response and deformation failure of F-type socket joint in a rectangular pipe jacking tunnel under different geologic conditions
Authors: Xu Youjun , Zhao Zhengrong , Zhang Chao , Zhang Xu , Pang Yuekui 
Published: June 2023

Abstract
The shear mechanical properties of F-type socket joints in rectangular pipe jacking tunnels are currently unknown. To investigate the shear mechanical response and deformation failure of the F-type socket joint in rectangular pipe jacking tunnels under different foundation coefficients, a laboratory joint test and numerical simulation method were used, considering the structural features of the joint. The results showed that during shear deformation of pipe sections, the intermediate pipe is damaged centrosymmetrically. The deformation process of a joint subjected to shear consists of four stages: gap closure, elastic growth, shear strengthening, and yield failure. The ultimate shear capacity of the joint increases by 25–34% for every 3 mm increase in the steel ring thickness. The chamfer yield damage area comprises approximately 15% of the steel ring, while the concrete damage area comprises approximately 40%. The failure characteristics of the joint are primarily manifested as drum and warp of the steel ring or cracking of the weld, and the concrete at the joint is crushed and shows evidence of cracks.
Blended Smartphone Intervention for Patients in Opioid Maintenance Treatment in Iran: Protocol for a Randomized Controlled Trial
Authors: Boumparis Nikolaos , Noroozi Alireza , Naghizadeh Eisa , Meyer Andreas , Wenger Andreas , Rahimi-Movaghar Afarin , Schaub Michael P. 
Published: June 2023

Abstract
Background
The pattern of substance use in Iran is characterized by a high prevalence of opioid use and opioid use disorder (OUD). Although opioid maintenance therapy (OMT) has been introduced in Iran, approximately 50% of people with opioid use disorder remain unreached. Moreover, psychosocial treatment of OUD and common mental health symptoms during OMT is limited. Digital interventions have been shown to improve psychological distress, depression, anxiety, and post-traumatic stress disorder symptoms. In addition, providing psychoeducation and risk reduction counseling to prevent communicable diseases like HIV and infectious hepatitis is common via the Internet. However, despite these promising advances, no smartphone intervention in OMT has been investigated for the treatment of OUD and common comorbid mental health symptoms.
Objective
We examine the effectiveness of adding a blended smartphone intervention based on community reinforcement approach, motivational interviewing- and cognitive behavioral therapy compared to OMT as usual that aims to improve OMT outcomes and addresses common mental health symptoms in OMT patients in Iran.
Method
Adults with opioid dependence entering 8 treatment centers in Tehran, Iran will be randomly assigned to receive either OMT plus a smartphone intervention or OMT as usual. The primary outcomes will be the percentage of negative urine tests for illicit, non-prescribed use of opioids (opium, heroin, tramadol) and treatment retention. Secondary outcomes will include the longest period of abstinence from the illicit, non-prescribed use of opioids (opium, heroin, and tramadol) confirmed by urine samples, changes in communicable disease risk-taking behaviors, changes in stress and common mental health symptoms, and client satisfaction. Data analysis will follow the intention-to-treat principle and employ (generalized) linear mixed models.
Discussion
This study will provide substantial knowledge for designing effective blended interventions for OUD. Moreover, it will investigate if treatment retention and OMT-related outcomes and common mental health symptoms can be improved by adding a smartphone intervention to OMT.
Trial Registration
https://en.irct.ir/trial/53578
Engineering of Nickel, Cobalt Oxides and Nickel/Cobalt Binary Oxides by Electrodeposition and Application as Binder Free Electrodes in Supercapacitors
Authors: Abbas Qaisar , Khurshid Hafsa , Yoosuf Rahana , Lawrence Jonathan , Issa Bashar A , Abdelkareem Mohammad Ali , Olabi Abdul Ghani 
Published: June 2023

Abstract
Cobalt oxide, nickel oxide and cobalt/nickel binary oxides were synthesised by electrodeposition. To fine tune composition of CoNi alloys, growth parameters including voltage, electrolyte pH/concentration and deposition time were varied. These produced nanomaterials were used as binder free electrodes in supercapacitor cells and tested using three electrode setup in 2M KOH aqueous electrolyte. Cyclic voltammetry and galvanostatic charge/discharge were used at different scan rates (5-100mV/s) and current densities (1-10A/g) respectively to evaluate the capacitive performance. Electrochemical impedance spectroscopy was used to analyse the resistive behaviour of these electrodes in frequency range of 100kHz to 0.01Hz at applied voltage of 10mV. Binary oxide electrode displayed superior electrochemical performance with the specific capacitance of 176F/g at current density of 1A/g. This hybrid electrode also displayed capacity retention of over 83% after 5,000 charge/discharge cycles. Cell displayed low solution resistance of 0.35Ω along with high energy density of 29Wh/kg at power density of 733W/kg for CoNi composite. The proposed facile approach to synthesise binder free blended metal electrodes can result in enhanced redox activity of pseudocapacitive materials. Consequently, fine tuning of these materials by controlling the cobalt and nickel ratios can assist in broadening their applications in electrochemical energy storage in supercapacitors.
Fatty acid analysis reveals variations in the diet of shallow-water spionids (Annelida: Polychaeta: Spionidae)
Authors: Zhukova Natalia 
Published: June 2023

Abstract
Abstract
The fatty acid (FA) composition of spionids co-occurring in shallow-water and occupying various habitats was analyzed to characterize the spectrum and diversity of their food sources and elucidate their feeding habits and trophic relationships. These included free-living and tube-dwelling species, and also opportunistic and specialized shell-borers and their hosts. A plot of principal component analysis (PCA) scores based on the FA compositions of nine spionid species recognized five distinct groups with different feeding strategies and revealed differences not only between the species, but also within the same species from various habitats. The predominance of 20:5n-3, C16 polyunsaturated FAs, and 16:1n-7 in suspension feeders indicated that they feed on diatoms. Benthic dinoflagellates and diatoms, as well as bacteria, constituted a major part in the diet of deposit feeders. On the basis of high 22:6n-3 and 18:1n-9 contents, Dipolydora commensalis was attributed to omnivorous with a predominance of animal dietary input. The selective detritivore Polydora glycymerica relies on a microbial food web. FAs of Laonice aff. cirrata indicated the importance of foraminifera in the diet of this subsurface feeder. Polychaetes were found to have a capability of changing their feeding habits depending on habitat. Specialized shell-borers adapt their feeding habits to the host’s diet. The results obtained provide evidence that these species actually represent several non-overlapping groups characterized by different feeding strategies, which leads to weakening of their competitive relationships. Partitioning of food sources is regarded as one of the main mechanisms explaining the successful coexistence of spionid species.
Gilded Vaterite Optothermal Transport in a Bubble
Authors: Gilad Hod , Barhum Hani , Ushkov Andrey , Machnev Andrey , Ofer Daniel , Bobrovs Vjačeslavs , Ginzburg Pavel 
Published: June 2023

Abstract
Laser beams, capable to control the mechanical motion of micron-scale objects, can serve as a tool, enabling investigations of numerous interaction scenarios under full control. Beyond the pure electromagnetic interactions, giving rise to conventional gradient forces and radiation pressure, environment-induced thermal effects can play a role and, in certain cases, govern the dynamics. Here we demonstrate a thermocapillary Marangoni effect, which is responsible for creating long-range few hundreds of nano-Newton forces, acting on a bubble around a ‘gilded vaterite’ nanoparticle. Decorating calcium carbonate spherulite (the vaterite) with gold nanoseeds allows tuning its optical absorption and, as a result, controlling its temperature in a solution. We demonstrate that keeping a balance between electromagnetic and thermal interactions allows creating of a stable micron-scale bubble around the particle and maintaining its size over time. The bubbles are shown to remain stable over minutes even after the light source is switched off. The bubbles were shown to swim toward laser focus over 400 of micrometer distances across the sample. Optothermal effects, allowing for efficient transport, stable bubble creation, and particle-fluid interaction control, can grant nano-engineered drug delivery capsules with additional functions toward a theragnostic paradigm shift.
Injectable Biocompatible Nanocomposites of Prussian Blue Nanoparticles and Bacterial Cellulose as a Safe and Effective Photothermal Cancer Therapy
Authors: Hong Hwichan , Kim MinKyu , Lee Wooseung , Jeon Miyeon , Lee Chaedong , Kim Hoonsub , Im Hyung-Jun , Piao Yuanzhe 
Published: June 2023

Abstract
Photothermal therapy (PTT) is a novel cancer treatment using a photoabsorber to cause hyperthermia to kill tumors by laser irradiation. Prussian blue nanoparticles (PB NPs) are considered as next-generation photothermal agents due to the facile synthesis and excellent absorption of near-infrared light. Although PB NPs demonstrate remarkable PTT capabilities, their clinical application is limited due to their systemic toxicity. Bacterial cellulose (BC) has been applied to various bio-applications based on its unique properties and biocompatibility. Herein, we design composites with PB NPs and BC as an injectable, highly biocompatible PTT agent (IBC-PB composites). Injectable bacterial cellulose (IBC) is produced through the trituration of BC, with PB NPs synthesized on the IBC surface to prepare IBC-PB composites. IBC-PB composites show in vitro and in vivo photothermal therapeutic effects similar to those of PB NPs but with significantly greater biocompatibility. Specifically, in vitro therapeutic index of IBC-PB composites is 26.5-fold higher than that of PB NPs. Furthermore, unlike PB NPs, IBC-PB composites exhibit no overt toxicity in mice as assessed by blood biochemical analysis and histological images. Hence, it is worth pursuing further research and development of IBC-PB composites as they hold promise as safe and efficacious PTT agents for clinical application.
The association between idiopathic pulmonary fibrosis and diabetes mellitus: a clinical retrospective study
Authors: Ji Tong , Chen Ranxun , Zhong Guanning , Lyu Wenting , Xu Qingqing , Jiang Hanyi , Gao Yujuan , Cao Min , Cai Hourong , Dai Jinghong 
Published: June 2023

Abstract
Background
Diabetes mellitus (DM) has been found to be related to lung fibrosis. However, the relationship between DM and idiopathic pulmonary fibrosis (IPF) remains uncertain. In this study, we aimed to determine the prevalence of DM in IPF and whether DM is associated with survival in IPF.
Methods
415 IPF patients were classified as two groups based on whether they were combined with or without DM. The medical records were reviewed and the baseline characteristics and survival times were compared. The adjusted Cox proportional hazards model was used to investigate the risk factors predicting survival in IPF patients and patients combined with IPF and DM, respectively. Then we selected predictors to establish predictive model for mortality.
Results
The prevalence of DM in IPF patients was 25.54%. DM was associated with reduced survival time(P = 0.002). DM (hazard ratio [HR], 1.421; 95% CI, 1.010–1.980; P = 0.039), acute exacerbation (AE)(HR, 2.419; 95% CI, 1.704–3.434; P < 0.001) and antifibrotic drugs (HR, 0.297; 95% CI, 0.199–0.422; P < 0.001) were independent significant factors of mortality in IPF patients. We proposed a prediction model based on DM, AE, antifibrotic drugs to stratify the risk of 1-year mortality of IPF patients. A small internal validation cohort showed the odds of with DAA scores of 0, 1 and 2 were 6.25%, 12.50%, 85.71%, respectively (no patient scored 3). Further, in patients combined with IPF and DM, metformin treatment was associated with prolonged survival time (P = 0.040), and the sequence of diagnoses of IPF and DM (HR, 0.671; 95% CI, 0.363–1.240; P = 0.203) did not affect the mortality of patients combined with IPF and DM.
Conlusions
Our retrospective study showed that DM was prevalent and were an independent risk factor for predicting mortality in IPF patients. And we established a predictive model for the risk of 1-year mortality of IPF patients which need further validations.