bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxic screening of endophytic fungi associated with Catharanthus roseus
Authors: LAY CATERINA SISKADEWI , AGUSTINA IKRA , ASTUTI PUJI , HERTIANI TRIANA 
Published: June 2023

Abstract. Lay CS, Agustina I, Astuti P, Hertiani T. 2023. Cytotoxic screening of endophytic fungi associated with Catharanthus roseus. Biodiversitas 24: 2716-2722. Despite the advancements in cancer therapy, there is still a need for new anticancer agents. Due to the tremendous range of chemical compounds that endophytic fungi create, they have become a crucial component of the current drug development process. The aim of this study was to evaluate the cytotoxic potential of endophytic fungi associated with Catharanthus roseus (L.) G.Don collected from Yogyakarta, Indonesia. All fungal EtOAc extracts were tested against three human cancer cell lines, HeLa, T47D, MCF-7, and Vero cell lines. The potential fungal isolate was identified using molecular techniques. The results showed that a total of seven endophytic fungi were obtained from the different parts of C. roseus. Only one fungus (SCR 3) showed the best cytotoxic activitiy towards all three cell lines used, especially the MCF-7 cell line (IC50 130.90 ?g/mL, Selectivity Index=11.42). The findings of using LC-HRMS also revealed several exciting compounds such as genistein and D-(-)-Mannitol. The results showed that EtOAc extract of SCR 3 (Aspergillus sp.) induced apoptosis but did not affect the cell cycle. This study suggests that EtOAc extract of SCR 3 may be a potential source of secondary metabolites that can function as a new source of anticancer agents.
Evaluation of nutritional values of tree-forage legume leaves from Gunungkidul District, Indonesia
Authors: DARMA I NYOMAN GUNA , JAYANEGARA ANURAGA , SOFYAN AHMAD , BUDIARTILACONI ERIKA , RIDLA MUHAMMAD , HERDIAN HENDRA 
Published: June 2023

Abstract. Darma ING, Jayanegara A, Sofyan A, Budiartilaconi E, Ridla M, Herdian H. 2023. Evaluation of nutritional values of tree-forage legume leaves from Gunungkidul District, Indonesia. Biodiversitas 24: 2733-2744. This study aimed to assess the nutritional value of tree legume leaves found in Gunungkidul District. A completely randomized design with 19 treatments (legume species) and six replications (nylon bags) was employed. The study evaluated the chemical composition, in sacco dry and organic matter degradation characteristics, and their correlation with the chemical composition. The results indicated variations in the crude protein (CP) content (12.44% to 26.9%), crude fiber (CF) content (16.13% to 27.38%), total digestible nutrients (TDN) (71.10% to 88.17%), and total phenol content (2.47% to 21.87%) on a dry matter basis. Among the legume species, Indigofera tinctoria L. exhibited the highest soluble fraction (a) values for dry matter (42.89±1.15%) and organic matter (40.32±1.37%). Additionally, I. tinctoria displayed the highest potential degraded fraction values (a+b) for dry matter (88.33±0.90%) and organic matter (88.12±0.95%). The effective degradation of dry matter (EDDM) and organic matter (EDOM) were 64.57±0.48% and 63.78±0.68%, respectively. The correlation coefficients (r) between ash, CP, TDN, CF, total phenol, and EDDM and EDOM values were 0.54, 0.58, 0.53, -0.73, -0.53, and 0.52, 0.55, 0.52, -0.72, -0.56 respectively. In conclusion, tree-forage legume leaves from Gunungkidul District exhibited varying nutritional values, with I. tinctoria showing the highest values for effective dry and organic matter degradation compared to other tree legumes. The ash content, CP, and TDN demonstrated positive correlations with the EDDM and EDOM values, whereas the CF content and total phenol exhibited negative correlations with the EDDM and EDOM values of tree legume leaves.
Ethnobotany of food plants used by Minangkabau Community in Lima Puluh Kota District, West Sumatra, Indonesia
Authors: AGESTI ASIH RAHAYU AJENG , ARIYANTI NUNIK SRI , CHIKMAWATI TATIK , PURWANTO YOHANES 
Published: June 2023

Abstract. Agesti ARA, Ariyanti NS, Chikmawati T, Purwanto Y. 2023. Ethnobotany of food plants used by Minangkabau Community in Lima Puluh Kota District, West Sumatra, Indonesia. Biodiversitas 24: 2756-2767. The Minangkabau tribe is well known for its culture and cuisine, but documents on their knowledge and wisdom in the utilization of food plants are still limited. This study aims to inventory plant species used for food by Minangkabau community, assess their importance and economic value, and analyze the important landscapes where the food plants are obtained. This study was conducted in six villages representing different level of modernization in Lima Puluh Kota District, West Sumatra which is one of the centers of origin of Minangkabau ancestral. Data were collected through open, semi-structured and structured interviews with key informants and respondents. The Pebble Distribution Method (PDM) is used for scoring activities. Data were analyzed to show the use values (UVs), local user's value index (LUVI), index of cultural significance (ICS), and economic value of the food plants. The interviewed informants mentioned 154 plant species from 51 families were used for food. Cocos nucifera L. has the most various uses as food (highest UVs) and is the most important cultivated plant for the Minangkabau culture (ICS) in all the six villages. The most important food plant species based on a community perspective (highest LUVI) is Oryza sativa L. The wild plants having the highest LUVI and ICS differed among the villages. Plants with high economic value include O. sativa, Durio zibethinus Murray and Citrus aurantium L. The landscapes considered important are paddy fields and fields because they provide staple food. Information from this study can support food diversification to achieve food security based on the local knowledge on the diversity of food plant species and potential landraces in Minangkabau community.
Environmental DNA (eDNA) metabarcoding and fish visual census reveals the first record of Doboatherina magnidentata in the Philippines
Authors: BAUTISTA JOHN ARLU , MANUBAG JANECE JEAN , SUMAYA NANETTE HOPE , MARTINEZ JOEY GENEVIEVE , TABUGO SHARON ROSE 
Published: June 2023

Abstract. Bautista JA, Manubag JJ, Sumaya NH, Martinez JG, Tabugo SR. 2023. Environmental DNA (eDNA) metabarcoding and fish visual census reveals the first record of Doboatherina magnidentatain the Philippines. Biodiversitas 24: 3063-3072. Biodiversity monitoring is the cornerstone for conserving marine fish species. However, classical methods, like the Fish Visual Census (FVC), are often limited due to sampling difficulties, the occurrence of rare and cryptic organisms, and reliance on a taxonomic expert for species identification. Recently, environmental DNA (eDNA) metabarcoding has been suggested as a non-invasive, powerful tool for biomonitoring. This study evaluates the eDNA approach as complementary tool for the FVC data in species detection and identification of important marine fishes from the marine sanctuary of Dalipuga, Iligan City, Philippines. The findings obtained through the eDNA approach provide insights into identifying significant fish species. Notably, the presence of the Hippocampus kuda Bleeker, 1852 (yellow seahorse), categorized as a vulnerable and threatened species, was detected. Additionally, the study identified Herklotsichthys quadrimaculatus Rüppell, 1837 (bluestripe herring), a native species to the Philippines that may pose potential risks to humans and the ecological balance. Furthermore, two demersal fish species, namely Large-scale whiting (Sillaginops macrolepis Bleeker, 1858) and Large-scale mullet (Planiliza macrolepis Smith, 1846), were also detected. The eDNA approach also delineated the morphologically cryptic fishes from Scaridae (parrotfishes) and Mugilidae (mullet fish) taxa to the species level. The highlight of this study was the detectionof the new Indo-pacific atherinomorine fish species Doboatherina magnidentata, which to the best of our knowledge, was the first record in the Philippine marine waters. Despite the efficiency of the eDNA metabarcoding in fish species detection and identification, the viability of eDNA in the marine environment and biases of the primer limit this method. Thus, the classical method must complement the molecular approach for better taxonomic resolution and community analysis. Future studies were also recommended to use a multigene eDNA approach to improve taxonomic sensitivity and reduce primer biases.
Surviving critical COVID-19: How functionality, physical, mental and cognitive outcomes evolve?
Authors: Teixeira-Vaz Ana , Rocha José Afonso , Oliveira Mafalda , Simões-Moreira Tiago , Reis David Almeida e , Silva Ana Isabel , Paiva José Artur 
Published: June 2023

PurposeTo analyze the long-term consequences of critical COVID-19, regarding physical, mental, cognitive and functional impairments, and to describe its evolution through time.MethodsProspective cohort study, with consecutive inclusion of patients admitted due to SARS-CoV-2 to intensive care units(ICU) of a tertiary-care center, between May/2020 and September/2021. All included patients were included in Physical and Rehabilitation Medicine(PRM) inpatient programs during ICU stay. Eligible patients were evaluated on PRM appointments 6 and 12 months after ICU discharge. In each visit, physical examination and a predefined set of scales were applied, aiming to comprehensively evaluate the three domains (physical, mental and cognitive) of post-intensive care syndrome and the patients’ functionality. Statistical analysis encompassed descriptive and univariate analysis.ResultsA total of 42 patients were included: 66.7% males, mean age of 62 yo. In the physical domain, 6 months after ICU discharge, there was a significant reduction in quality of life (p-value = 0.034), muscle strength (p-value = 0.002), gait ability (p-value<0.001) and balance (p-values<0.001) and increased fatigue levels (p-value = 0.009), in comparison with reference values. Yet, a significative positive evolution was observed in all referred subdomains (p-values<0.05). Nevertheless, 12 months after discharge, muscle strength (p-value = 0.001), gait (p-value<0.001) and balance (p-value<0.001) were still significantly compromised. Regarding the mental domain, both at 6 and 12 months after discharge, the levels of anxiety and depression were significantly increased (p-values<0.001). Nonetheless, a positive evolution was also found (p-values<0.02). Cognitive performance was significantly impaired in comparison with reference values, both at 6 and 12 months (p-value<0.001). Yet, a global improvement was also depicted (p-value = 0.003). Six months after ICU discharge, 54.8% were autonomous in activities of daily living, a value that improved to 74.0% in the subsequent 6 months (p-value = 0.002).ConclusionCritical COVID-19 survivors present significant physical, mental and cognitive impairments 6 and 12 months after ICU discharge, despite their positive evolution through time.
Variability of response on prophylactic prasugrel for endovascular treatment of intracranial aneurysms: Clinical implications
Authors: Hong Noah , Kim Seung Bin , Yang Hee-Jin , Son Young-Je 
Published: June 2023

Background and purpose
Prophylactic prasugrel for endovascular treatment of intracranial aneurysms has been introduced and increased, but HTPR (high on-treatment platelet reactivity) or LTPR (low on-treatment platelet reactivity) of prasugrel is not uncommon in clinical circumstances. To investigate the predisposing factors of HTPR and LTPR on prasugrel premedication in the neurointerventional field and to determine its clinical implications.

Materials and methods
Between February 2016 and December 2020, 191 patients treated with coil embolization using prophylactic prasugrel in 234 intracranial aneurysms were the final candidates for this study. Patient and aneurysm characteristics, clinical status, and laboratory study values were carefully reviewed retrospectively. We performed risk factor analyses for HTPR and LTPR on prasugrel.

Results
Ultimately, 20 patients (10.5%) had HTPR, and 74 patients (38.7%) were categorized as having LTPR. In multivariable analyses, the factors related to HTPR were BMI (adjusted OR 1.21, 95% CI 1.04–1.41, p = 0.01), history of antithrombotics (adjusted OR 3.79, 95% CI 1.39–10.34, p = 0.01), and hematocrit (adjusted OR 0.91, 95% CI 0.84–0.99, p = 0.03). Low BMI was the only risk factor for LTPR (adjusted OR 0.84, 95% CI 0.76–0.94, p = 0.001).

Conclusion
In the neurointerventional field, high BMI and prior use of antithrombotic agents were related to HTPR, and low BMI was associated with LTPR on prophylactic prasugrel. High hematocrit levels decreased the risk of HTPR. When preparing endovascular treatment for intracranial aneurysms, attention to patients with these clinical features is required to address the possibility of ischemic or bleeding complications.
Self-reported emotional and behavioral problems among school-going adolescents in Nepal—A cross-sectional study
Authors: Adhikari Sirjana , Ma Jasmine , Shakya Suraj , Brøndbo Per Håkan , Handegård Bjørn Helge , Javo Anne Cecilie 
Published: June 2023

Background
Studies on self-reported emotional and behavioral problems (EBPs) among adolescents are still sparse in many low- and middle-income countries. In Nepal, no such studies have been performed on a larger scale, and little is known about self-reported EBPs in the adolescent population.

Methods
This cross-sectional, school-based study on EBPs included 1904 adolescents aged 11–18 years, enrolled in government and private schools located in 16 districts in Nepal. The Nepali version of the Youth Self Report form was used to assess self-reported EBPs, and the Teacher Report Form was used to assess academic performance. Analysis of variance (ANOVA) was used for gender comparisons on adolescents’ EBPs and on academic competence. Multiple regression analysis was done to explore correlates of self-reported EBPs.

Results
The overall prevalence of self-reported EBPs was 14.2%; 15.6% in boys and 12.9% in girls. The mean Total Problems score was 39.27 (standard deviation = 24.16); no gender differences were observed. Boys scored higher on Externalizing Problems and girls scored higher on Internalizing Problems. The effect sizes for gender comparisons were small with Hedges’ g ranging from -0.29 to 0.28. Physical illness and negative/traumatic life events were positively correlated with self-reported EBPs, whereas academic performance was negatively correlated. However, the effect sizes were small (η2 < 0.02).

Conclusion
This study helps to narrow the knowledge gap on the prevalence, magnitude, and types of self-reported EBPs in Nepali adolescents. It demonstrated an association between self-reported EBPs and academic performance and linked self-reported EBPs to other factors such as negative/traumatic life events and physical illness. The findings might assist health authorities in the planning of mental health services and may also provide valuable background information to clinicians dealing with adolescent mental health problems.
Risk factors for medical device-related pressure injury in ICU patients: A systematic review and meta-analysis
Authors: Gou Ling , Zhang Zhiqin , A. Yongde 
Published: June 2023

Background
Medical device-related pressure injury (MDRPI) in intensive care unit (ICU) patients is a serious issue. We aimed to evaluate the risk factors for MDRPI associated with ICU patients through systematic review and meta-analysis, and provide insights into the clinical prevention of MDRPI.

Methods
We searched PubMed, Embase, Web of Science, China National Knowledge Infrastructure (CNKI), WanFang Database, and China BioMedical Literature Database (CBM) (from inception to January 2023) for studies that identified risk factors of MDRPI in ICU patients. In order to avoid the omission of relevant literature, we performed a secondary search of the above database on February 15, 2023. Meta-analysis was performed using Revman 5.3.

Results
Fifteen studies involving 4850 participants were selected to analyze risk factors for MDRPI in ICU patients. While conducting a meta-analysis, we used sensitivity analysis to ensure the reliability of the results for cases with significant heterogeneity among studies. When the source of heterogeneity cannot be determined, we only described the risk factor. The risk factors for MDRPI in ICU patients were elder age (OR = 1.06, 95% CI: 1.03–1.10), diabetes mellitus (OR = 3.20, 95% CI: 1.96–5.21), edema (OR = 3.62, 95% CI: 2.31–5.67), lower Braden scale score (OR = 1.22, 95%CI: 1.11–1.33), higher SOFA score (OR = 4.21, 95%CI: 2.38–7.47), higher APACHE II score (OR = 1.38, 95%CI: 1.15–1.64), longer usage time of medical devices (OR = 1.11, 95%CI: 1.05–1.19), use of vasoconstrictors (OR = 6.07, 95%CI: 3.15–11.69), surgery (OR = 4.36, 95% CI: 2.07–9.15), prone position (OR = 24.71, 95% CI: 7.34–83.15), and prone position ventilation (OR = 17.51, 95% CI: 5.86–52.36). Furthermore, we found that ICU patients who used subglottic suction catheters had a higher risk of MDRPI, whereas ICU patients with higher hemoglobin and serum albumin levels had a lower risk of MDRPI.

Conclusion
This study reported the risk factors for MDRPI in ICU patients. A comprehensive analysis of these risk factors will help to prevent and optimize interventions, thereby minimizing the occurrence of MDRPI.
COVID-19 symptom load as a risk factor for chronic pain: A national cross-sectional study
Authors: Romeiser Jamie L. , Morley Christopher P. , Singh Sunitha M. 
Published: June 2023

IntroductionEmerging evidence suggests that a COVID-19 infection with a high initial severity may be associated with development of long-COVID conditions such as chronic pain. At the population level, it is unknown if severity of a COVID-19 infection might be a new risk factor for chronic pain above and beyond the traditional slate of pre-established risk factors. The purpose of this study is to examine whether COVID-19 severity of infection may be a new risk factor for chronic pain.MethodsUsing data from the 2021 National Health Interview Survey (n = 15,335), this study examined the adjusted odds of experiencing high frequency levels of pain in the past 3 months for those who reported no/mild symptoms from a COVID-19 infection, and those reporting moderate/severe symptoms from COVID-19, compared to those never infected. A 1:1:1 propensity score matched analysis was also performed to examine the odds of pain.ResultsPrevalence of pain was higher in the moderate/severe symptom group compared to the no infection group (25.48% vs 19.44%, p <0.001). Both the adjusted model (odds ratio [OR] = 1.28, 95% confidence interval [CI] = 1.09, 1.51) and matched model (OR = 1.45, CI = 1.14, 1.83) revealed higher odds of pain for those with moderate/high COVID-19 symptoms compared to no infection.ConclusionsA moderate/highly symptomatic COVID-19 infection may be a new risk factor for chronic pain. As the absolute number of severe COVID-19 infections continues to rise, overall prevalence of chronic pain may also increase. While knowledge continues to unfold on long-haul symptoms, prevention of severe infections remains essential.
Advanced gynecological cancer: Quality of life one year after diagnosis
Authors: Jónsdóttir Björg , Wikman Anna , Sundström Poromaa Inger , Stålberg Karin 
Published: June 2023

Objective
Gynaecological cancer treatment impacts women’s physical and psychological health. Our objective was to examine quality of life (QoL) in women with advanced gynaecological cancer at diagnosis and one year later, and to identify sociodemographic and clinical characteristics associated with QoL.

Methods
Women with endometrial, ovarian or cervical cancer treated in Uppsala, Sweden 2012–2019 were included. FIGO stage ≥II was considered advanced gynaecological cancer, whereas women in FIGO stage I were used as a control group. QoL was assessed with SF-36. We obtained information on sociodemographic and clinical characteristics from medical records and health questionnaires. Differences in QoL domains were tested with t-tests, a mixed model ANOVA and multiple linear regression analyses.

Results
The study population (n = 372) included 150 (40.3%) women with advanced gynaecological cancer. At diagnosis, women with advanced cancer reported lower physical (71.6 vs 81.8 (mean) p<0.05) and role functioning/physical scores (62.6 vs 77.2 (mean) p<0.05) than women in FIGO stage I. One year later, women with advanced cancer reported higher scores in the mental health domain (78.3 vs 73.2 (mean) p<0.05) than women in FIGO stage I. However, no difference was found in the QoL scores of women with advanced disease one year after diagnoses when stratified by diagnosis. Women with a history of psychiatric illness and higher BMI reported poorer physical and mental QoL at follow-up, while advanced stage, level of education and smoking were not associated with QoL.

Conclusion
Women with advanced gynaecological cancer have equally good QoL one year after diagnosis as women with limited disease. Women with previous psychiatric illness and high BMI, are at risk of impaired physical and mental health.
Adequacy of endoscopic recognition and surveillance of gastric intestinal metaplasia and atrophic gastritis: A multicentre retrospective study in low incidence countries
Authors: Honing Judith , Keith Tan W. , Dieninyte Egle , O’Donovan Maria , Brosens Lodewijk , Weusten Bas , di Pietro Massimiliano 
Published: June 2023

Background
Gastric atrophy (GA) and gastric intestinal metaplasia (GIM) are precursor conditions to gastric adenocarcinoma (GAC) and should be monitored endoscopically in selected individuals. However, little is known about adherence to recommendations in clinical practice in low-risk countries.

Objective
The aim of this study was to evaluate endoscopic recognition and adequacy of surveillance for GA and GIM in countries with low GAC prevalence.

Methods
We retrospectively analysed patients diagnosed with GIM or GA in three centers in The Netherlands and UK between 2012 and 2019. Cases with GIM and/or GA diagnosis at index endoscopy were retrieved through systematic search of pathology databases using ‘gastric’ and ‘intestinal metaplasia’ or ‘atrophy’ keywords. Endoscopy reports were analysed to ascertain accuracy of endoscopic diagnoses. Adequacy of surveillance was assessed following histological diagnosis at the index endoscopy based on ESGE guidelines published in 2012.

Results
We included 396 patients with a median follow-up of 57.2 months. Mean age was 66 years and the rates of antrum-predominant versus extensive GIM were comparable (37% vs 38%). Endoscopic recognition rates were 48.5% for GA and 16.3% for GIM. Surveillance was adequately carried out in 215 of 396 patients (54.3%).

Conclusion
In countries with a low incidence of GAC, the rate of endoscopic recognition of gastric pre-cancerous lesions and adherence to surveillance recommendation are low. Substantial improvement is required in endoscopic training and awareness of guidelines recommendation in order to optimise detection and management of pre-malignant gastric conditions.