bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative genomics reveals the emergence of an outbreak-associatedCryptosporidium parvumpopulation in Europe and its spread to the USA
Authors: Bellinzona Greta , Nardi Tiago , Castelli Michele , Batisti Biffignandi Gherard , Betson Martha , Blanchard Yannick , Bujila Ioana , Chalmers Rachel , Davidson Rebecca , D’Avino Nicoletta , Enbom Tuulia , Gomes Jacinto , Karadjian Gregory , Klotz Christian , Östlund Emma , Plutzer Judith , Rimhanen-Finne Ruska , Robinson Guy , Sannella Anna Rosa , Sroka Jacek , Stensvold Christen Rune , Troell Karin , Vatta Paolo , Zalewska Barbora , Bandi Claudio , Sassera Davide , Cacciò Simone M. 
Published: September 2023

AbstractThe zoonotic parasiteCryptosporidium parvumis a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphicgp60gene enabled the classification ofC. parvumisolates into multiple groups (e.g. IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water-and food-borne outbreaks. In this study, we generated new whole genome sequence (WGS) data from 123 human-and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China and the USA (n=72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analysed a dataset from 141 isolates from the zoonotic groups IIa (n=119) and IId (n=22). Based on 28,047 high quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: isolates from China (IId) and Egypt (IIa and IId) formed population 1, a minority of European isolates (IIa and IId) formed population 2, while the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the USA (IIa) clustered in population 3. Based on analyses of the population structure, population genetics and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the UK, reach the USA where it also expanded. In addition, genetic exchanges between different populations led to the formation of mosaic genomes. The reason(s) for the successful spread of population 3 remained elusive, although genes under selective pressure uniquely in this population were identified.
Genetic variation exists withinZea maysto influence unsustainable nitrogen cycling microbiome function
Authors: Favela Alonso , Bohn Martin O. , Kent Angela D. 
Published: September 2023

AbstractOveruse of synthetic nitrogen fertilizers in agroecosystems causes environmental pollution and human harm at a global level. Nitrogenous fertilizers provide a short-lived benefit to crops in the agroecosystem, but stimulate microbially-mediated nitrification and denitrification, processes that result in N pollution, greenhouse gas (GHG) production, and reduced soil fertility. Recent advances in plant microbiome science suggest that plants can modulate the composition and activity of rhizosphere microbial communities. These rhizosphere communities act as an extended phenotype, primed by genetic variation in the plant host. Genetic variation in traits (e.g., plant secondary metabolites, root architecture, immune system, etc.) act as mechanistic selective agents on the composition of the microbiome. Here we attempted to determine whether genetic variation exists inZea maysfor the ability to influence the extended phenotype of rhizosphere soil microbiome composition and function. Specifically, we determined whether plants’ influence on soil nitrogen cycling activities was altered by plant genetics and thereby allowing it to be incorporated into breeding practices. To capture an extensive amount of genetic diversity within maize we sampled the rhizosphere microbiome of a germplasm chronosequence that included ex-PVP inbreds, hybrids, and teosinte (Z. maysssp. mexicanaandZ. maysssp.parviglumis). We observed that potential N cycling processes were influenced by plant genetics. Teosinte and some hybrid genotypes supported microbial communities with lower potential nitrification and potential denitrification activity in the rhizosphere, while inbreds stimulated/did not inhibit these undesirable N-cycling activities. These potential differences translated to functional differences in N2O production, with teosinte plots producing less GHG than maize plots. Furthermore, within theseZeacultivars we found that plant genetics explained a significant amount of variation in the microbiome, particularly among different nitrification and denitrification functional genes within the community. We found that potential nitrification, potential incomplete denitrification, and overall denitrification rates, but not abundance of N-cycling genes of rhizosphere soils were influenced by growth stage and plant genetics. Taken together, these results suggest that crop selection can lead to changes in root phenotypes that could suppress unsustainable N-cycling processes. Reintroducing stress-adapted and “wild” root characteristics into modern germplasm may be a way to manipulate soil microbiomes at both a composition and functional level to improve sustainability.
Microbial Gladiators: Unraveling the dynamics of carbon substrate competition among heterotrophic microbes
Authors: McNichol Samuel M. , Sanchez-Quete Fernando , Loeb Stephanie K. , Teske Andreas , Shah Walter Sunita R. , Mahmoudi Nagissa 
Published: September 2023

AbstractGrowing evidence suggests that interactions among heterotrophic microbes influence the efficiency and rate of organic matter turnover. These interactions are dynamic and shaped by the composition and availability of resources in their surrounding environment. Heterotrophic microbes inhabiting marine environments often encounter fluctuations in the quality and quantity of carbon inputs, ranging from simple sugars to large, complex compounds. Here, we experimentally tested how the chemical complexity of carbon substrates affects competition and growth dynamics between two heterotrophic marine isolates. We tracked cell density using species-specific PCR assays and measured rates of microbial CO2production along with associated isotopic signatures (13C and14C) to quantify the impact of these interactions on organic matter remineralization. The observed cell densities revealed substrate-driven interactions: one species exhibited a competitive advantage and quickly outgrew the other when incubated with a labile compound while both species seemed to coexist harmoniously in the presence of more complex organic matter. Rates of CO2respiration revealed that co-incubation of these isolates enhanced organic matter turnover, sometimes by nearly twofold, compared to their incubation as mono-cultures. Isotopic signatures of respired CO2indicated that co-incubation resulted in a greater remineralization of macromolecular organic matter. These results demonstrate that simple substrates promote competition while high substrate complexity reduces competitiveness and promotes the partitioning of degradative activities into distinct niches, facilitating coordinated utilization of the carbon pool. Taken together, this study yields new insight into how the quality of organic matter plays a pivotal role in determining microbial interactions within marine environments.
AsCDPR: a novel framework for ratings and personalized preference hotel recommendation using cross-domain and aspect-based features
Authors: Wang Hei-Chia , Justitia Army , Wang Ching-Wen 
Published: September 2023

PurposeThe explosion of data due to the sophistication of information and communication technology makes it simple for prospective tourists to learn about previous hotel guests' experiences. They prioritize the rating score when selecting a hotel. However, rating scores are less reliable for suggesting a personalized preference for each aspect, especially when they are in a limited number. This study aims to recommend ratings and personalized preference hotels using cross-domain and aspect-based features.Design/methodology/approachWe propose an aspect-based cross-domain personalized recommendation (AsCDPR), a novel framework for rating prediction and personalized customer preference recommendations. We incorporate a cross-domain personalized approach and aspect-based features of items from the review text. We extracted aspect-based feature vectors from two domains using bidirectional long short-term memory and then mapped them by a multilayer perceptron (MLP). The cross-domain recommendation module trains MLP to analyze sentiment and predict item ratings and the polarities of the aspect based on user preferences.FindingsExpanded by its synonyms, aspect-based features significantly improve the performance of sentiment analysis on accuracy and the F1-score matrix. With relatively low mean absolute error and root mean square error values, AsCDPR outperforms matrix factorization, collaborative matrix factorization, EMCDPR and Personalized transfer of user preferences for cross-domain recommendation. These values are 1.3657 and 1.6682, respectively.Research limitation/implicationsThis study assists users in recommending hotels based on their priority preferences. Users do not need to read other people's reviews to capture the key aspects of items. This model could enhance system reliability in the hospitality industry by providing personalized recommendations.Originality/valueThis study introduces a new approach that embeds aspect-based features of items in a cross-domain personalized recommendation. AsCDPR predicts ratings and provides recommendations based on priority aspects of each user's preferences.
Role of psychosocial safety climate in job satisfaction and emotional exhaustion: mediating role of effort–reward imbalance
Authors: Tripathi Padma , Priyadarshi Pushpendra , Kumar Pankaj , Kumar Sushil 
Published: September 2023

PurposeThe purpose of this paper is to study the role of psychosocial safety climate (PSC) on job satisfaction and emotional exhaustion among employees and to examine the mediating role of effort–reward imbalance (ERI) in this relationship.Design/methodology/approachThis study investigated a mediation model with ERI explaining the relationship between PSC and the outcome variables using a sample of 441 employees of information technology (IT) organizations in India. The data were analyzed using structural equation modeling (SEM) techniques with LISREL (linear structural relations) 8.72 software.FindingsThe results suggest that PSC significantly influences the employees' experiences of job satisfaction and emotional exhaustion. Also, ERI was demonstrated as a significant intervening construct with full mediation of the PSC–emotional exhaustion relationship and partial mediation of the PSC–job satisfaction relationship.Research limitations/implicationsThe study provides substantial results and arguments to encourage organizational-level commitment for psychosocial risk management through distributive fairness and reciprocity in the form of ERI to foster positive attitudes and prevent negative health and psychological outcomes. The cross-sectional nature of the study limits generalizability but contributes to the literature on work stress in a developing country's context.Originality/valueThe study demonstrates how employee outcomes like job satisfaction and emotional exhaustion often result from their perceptions of inequity and imbalance at the workplace. Further, the study builds a strong case for helping organizations contribute to the United Nations (UN) 2030 sustainability goals by empirically establishing the crucial role of top management's commitment and prioritization of employee psychosocial health and safety for designing primary stress-management initiatives for sustainable psychosocial risk prevention and management.
Structural and cultural competencies in maternity care for ethnic minority and migrant women: practitioner perspectives from Aotearoa New Zealand
Authors: Sharma Vartika , Singh Nikki , Chiang Annie , Paynter Janine , Simon-Kumar Rachel 
Published: September 2023

Purpose
With global migration, the number of ethnic minority and migrant women receiving maternity health care in dominantly Anglo-European societies has increased significantly but they consistently have among the worst pregnancy and maternal outcomes. This paper aims to analyse gaps in structural (migration-related inequalities) and cultural (responsiveness to ethno-cultural practices) competencies among maternal health practitioners in Aotearoa New Zealand (NZ).

Design/methodology/approach
Using a semi-structured interview guide, in-depth interviews were conducted with 13 maternal health practitioners in NZ. Data were analysed using a thematic analysis framework.

Findings
The results highlight significant barriers around language and communication, cultural stereotyping by professionals, ethnic women’s own constraints around family and cultural expectations and their lack of knowledge about reproductive health. In addition, practitioners’ own ethnic differences are inseparable from their approach to structural and cultural competencies; there were instances of ‘over-’ or ‘under-’ reading of culture, practitioner constructions of ideal pregnancies and anti-racism concerns that shaped maternal care practices that were sensitive to, but also marginalised, ethnic migrant women who attended maternity services.

Originality/value
To the best of the authors’ knowledge, this is the only study in NZ that examines the impact of complex dynamics of migration and culture on knowledge, beliefs and values of practitioners, in context of their own personal biographies. Identifying strategies to improve the way diversity is practiced in hospital settings can be transformational in improving maternal outcomes for ethnic migrant women in NZ.
Robotic milling stability optimization based on robot functional redundancy
Authors: Gao Ke , Zhou Xiaoqin , Wang Rongqi , Fan Mingxu , Han Haochen 
Published: September 2023

Purpose
Compared with the high stiffness of traditional CNC machine tools, the structural stiffness of industrial robots is usually less than 1 N/µm. Chatter not only affects the quality of robotic milling but also reduces the accuracy of the milling process. The purpose of this paper is to reduce chatter in the robotic machining process.

Design/methodology/approach
First, the mode coupling chatter mechanism is analyzed. Then the milling force model and the principal stiffness model are established. Finally, the robot milling stability optimization method is proposed. The method considered functional redundancies, and a new robot milling stability index is proposed to improve the quality of milling operations.

Findings
The experimental results prove a significant reduction in force fluctuations and surface roughness after using the proposed robotic milling stability optimization method.

Originality/value
In this paper, a new robot milling stability index and a new robot milling stability optimization method are proposed. This method can significantly increase the milling stability and improve the milling quality, which can be widely used in the industry.
Leadership to promote patient safety culture in public hospitals managed by social health organizations
Authors: Pulzi Júnior Sérgio Antônio , Araujo Claudia Affonso Silva , Ferreira da Silva Mônica 
Published: September 2023

Purpose
This paper aims to identify the kind of internal climate leaders should offer health-care professionals to promote a patient safety culture in public hospitals managed by social health organizations in Brazil.

Design/methodology/approach
Two surveys were applied to health-care professionals working at three Brazilian public hospitals. The internal climate survey reached 1,013 respondents, and the patient safety culture survey reached 1,302 participants. Both factor and regression analyses were used to analyze the study model and determine how internal climate influences patient safety culture.

Findings
Results indicate that to promote a patient safety culture among health-care professionals, leaders should generate an internal climate based on trust to foster pride in working in the hospital. Possibly, the trust dimension is the most important one and must be developed to achieve job satisfaction and provide better services to patients.

Research limitations/implications
All the hospitals studied were managed by the same Organização Social de Saúde. Due to the limited responses concerning the respondents’ profiles, demographic variables were not analyzed.

Practical implications
This research reveals that the trust and pride dimensions can most strongly influence a positive patient safety culture, helping hospital leaders face this huge managerial challenge of consistently delivering high standards of patient safety.

Originality/value
This research studies the promotion of a patient safety culture in public hospitals managed by social health organizations, characterized by greater flexibility and autonomy in health-care management and by a greater need for accountability.
Intrinsic resistance to ROS1 inhibition in a patient with CD74‐ROS1 mediated by AXL overexpression
Authors: Peters Tara L. , Chen Nan , Tyler Logan C. , Le Anh T. , Dimou Anastasios , Doebele Robert C. 
Published: September 2023

AbstractBackgroundThe vast majority of patients with ROS1 positive non‐small cell lung cancer (NSCLC) derive clinical benefit from currently approved ROS1 therapies, including crizotinib and entrectinib. However, a small proportion of patients treated with ROS1 inhibitors fail to derive any clinical benefit and demonstrate rapid disease progression. The biological mechanisms underpinning intrinsic resistance remain poorly understood for oncogene‐driven cancers.MethodsWe generated a patient‐derived cell line, CUTO33, from a ROS1 therapy naive patient with CD74‐ROS1+ NSCLC, who ultimately did not respond to a ROS1 inhibitor. We evaluated a panel of ROS1+ patient‐derived NSCLC cell lines and used cell‐based assays to determine the mechanism of intrinsic resistance to ROS1 therapy.ResultsThe CUTO33 cell line expressed the CD74‐ROS1 gene fusion at the RNA and protein level. The ROS1 fusion protein was phosphorylated at baseline consistent with the known intrinsic activity of this oncogene. ROS1 phosphorylation could be inhibited using a wide array of ROS1 inhibitors, however these inhibitors did not block cell proliferation, confirming intrinsic resistance in this model and consistent with the patient's lack of response to a ROS1 inhibitor. CUTO33 expressed high levels of AXL, which has been associated with drug resistance. Combination of an AXL inhibitor or AXL knockdown with a ROS1 inhibitor partially reversed resistance.ConclusionsIn summary, we demonstrate that AXL overexpression is a mechanism of intrinsic resistance to ROS1 inhibitors.
Does the decline in Swedish adolescent drinking persist into early adulthood?
Authors: Kraus Ludwig , Loy Johanna K. , Olderbak Sally , Trolldal Björn , Ramstedt Mats , Svensson Johan , Törrönen Jukka 
Published: September 2023

AbstractBackground and aimsSweden has experienced a substantial decrease in adolescent drinking over the past decades. Whether the reduction persists into early adulthood remains unclear. Using survey data, the present study aimed to determine whether reductions in indicators of alcohol use observed among adolescents remain in early adulthood and whether changes in alcohol intake are consistent among light/moderate and heavy drinkers.DesignData from the Swedish monthly Alcohol Monitoring Survey (2001–20) were used to construct five 5‐year birth cohorts (1978–82, 1983–87, 1988–92, 1993–97 and 1998–2002).SettingSweden.ParticipantsA total of n = 52 847 respondents (48% females) aged 16 and 30 years were included in this study.MeasurementsFor both males and females, temporal changes in the prevalence of any drinking, the prevalence of heavy episodic drinking (HED) and total alcohol intake in the past 30 days in centilitres were analysed.FindingsThe prevalence of any drinking in more recent cohorts remained low until young people came into their early (females) and mid‐ (males) 20s. Male cohorts differed in the prevalence of HED across age, with the later cohorts showing lower odds than earlier cohorts (odds ratios between 0.54 and 0.66). Among females, no systematic differences between cohorts across age could be observed. Later male birth cohorts in light/moderate drinkers had lower alcohol intake than earlier cohorts (correlation coefficients between −0.09 and −0.54). No statistically significant cohort effects were found for male heavy drinkers. Although differences in alcohol intake among females diminished as age increased, the cohorts did not differ systematically in their level of alcohol intake.ConclusionsIn Sweden, the reduced uptake of drinking in adolescents appears to fade as people move into adulthood. Observed reductions in alcohol intake among light and moderate drinkers appear to persist into adulthood. More recent male cohorts show a lower prevalence rate of heavy episodic drinking.
The prognostic significance of lymphovascular invasion in cutaneous squamous cell carcinoma
Authors: Huang Sarah Suruo , Toon Christopher W. , Harish Varun 
Published: September 2023

AbstractBackgroundThe majority of cutaneous squamous cell carcinomas (cSCC) have a favourable prognosis. However, a subset of cases follow an aggressive disease course with progression to metastasis and death. Several histopathological parameters are associated with poor outcomes, but lymphovascular invasion (LVI) has not been well studied.ObjectiveTo assess the prognostic significance of LVI in cSCC and determine associations between LVI and cSCC.MethodsA retrospective review of 486 consecutive cases of cSCC over a 5‐year period from a single centre was stratified by the presence or absence of LVI. Logistic regression and multivariate survival analysis were used to determine associations of LVI and prognostic significance of LVI, respectively.FindingsLVI was present in 41 cases (9.2%). LVI was significantly associated with increasing depth of invasion, microanatomical tumour location (subcutis vs. dermis), and tumour dimensions (P < 0.05). Univariate survival analysis revealed significantly lower 2‐year overall survival rates for patients with LVI (37.1%) compared with those without (66.6%) (95% CI = 60.6–73.3, P < 0.001). LVI was also found to be an independent marker of poor disease‐specific survival (HR = 0.232 (95% CI = 0.090–0.600), P = 0.003), poor overall survival (HR 0.338 (95% CI = 0.184–0.623), P < 0.001) and poor disease‐free survival (HR 0.461 (95% CI = 0.230–0.923), P = 0.029) through multivariate analysis.ConclusionsThis study confirms that LVI is an independent poor prognosticator in cSCC, with significantly worse survival indices at 2 years. Future systems of risk stratification for cSCC should incorporate LVI.