bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dysregulation of Grainyhead‐like 3 expression causes widespread developmental defects
Authors: Deng Zihao , Butt Tariq , Arhatari Benedicta D. , Darido Charbel , Auden Alana , Swaroop Dijina , Partridge Darren D. , Haigh Katharina , Nguyen Thao , Haigh Jody J. , Carpinelli Marina R. , Jane Stephen M. 
Published: January 2023

AbstractBackgroundThe gene encoding the transcription factor, Grainyhead‐like 3 (Grhl3), plays critical roles in mammalian development and homeostasis. Grhl3‐null embryos exhibit thoraco‐lumbo‐sacral spina bifida and soft‐tissue syndactyly. Additional studies reveal that these embryos also exhibit an epidermal proliferation/differentiation imbalance. This manifests as skin barrier defects resulting in peri‐natal lethality and defective wound repair. Despite these extensive analyses of Grhl3 loss‐of‐function models, the consequences of gain‐of‐function of this gene have been difficult to achieve.ResultsIn this study, we generated a novel mouse model that expresses Grhl3 from a transgene integrated in the Rosa26 locus on an endogenous Grhl3‐null background. Expression of the transgene rescues both the neurulation and skin barrier defects of the knockout mice, allowing survival into adulthood. Despite this, the mice are not normal, exhibiting a range of phenotypes attributable to dysregulated Grhl3 expression. In mice homozygous for the transgene, we observe a severe Shaker‐Waltzer phenotype associated with hearing impairment. Micro‐CT scanning of the inner ear revealed profound structural alterations underlying these phenotypes. In addition, these mice exhibit other developmental anomalies including hair loss, digit defects, and epidermal dysmorphogenesis.ConclusionTaken together, these findings indicate that diverse developmental processes display low tolerance to dysregulation of Grhl3.
Portable motorized telescope system for wind turbine blades damage detection
Authors: Carnero Alejandro , Martín Cristian , Díaz Manuel 
Published: January 2023

AbstractWind turbines are among the fastest‐growing sources of energy production and the maintenance operations include regular inspection of their blades, causing considerable downtime and cost. In addition, the manual inspection process involves a great risk. To address this challenge, in this article a preventive maintenance system for wind turbines based on deep computational learning techniques is presented. This open‐source project aims to detect and classify possible surface damages on wind turbine blades to facilitate and improve the inspection of such infrastructures. The system consists of a stand‐alone Android application that makes use of convolutional neural networks for image processing, a portable telescope to take precise photographs of the turbine blades, and a motorized mount that allows the movement of the telescope. The application tries to carry out a complete sweep of the surface of the wind turbine blades in an autonomous way based on the predictions of neural network models and finally presents the defects found to the user. Thanks to this, maintenance time would be reduced and the risk of manual intervention would be avoided. Accuracies of around 97% for label predictions and 90% for bounding box coordinate predictions have been achieved on the validation dataset. The proposed low‐cost inspection system for detecting surface damages on blades has been experimentally validated in a real wind farm.
Triclosan and Methyl Triclosan in Prey Fish in a Wastewater-Influenced Estuary
Authors: Lin Diana , Hamilton Coreen , Hobbs James , Miller Ezra , Sutton Rebecca 
Published: January 2023

Abstract
While the antimicrobial ingredient triclosan has been widely monitored in the environment, much less is known about the occurrence and toxicity of its major transformation product, methyl triclosan. An improved method was developed and validated to effectively extract and quantify both contaminants in fish tissue and used to characterize concentrations in small prey fish in areas of San Francisco Bay where exposure to triclosan via municipal wastewater discharges was expected to be highest. Concentrations of triclosan (0.44–57 ng/g wet wt, median 1.9 ng/g wet wt) and methyl triclosan (1.1–200 ng/g wet wt, median 36 ng/g wet wt) in fish tissue decreased linearly with concentrations of nitrate in site water, used as indicators of wastewater influence. The total concentrations of triclosan and methyl triclosan measured in prey fish were below available toxicity thresholds for triclosan, but there are few ecotoxicological studies to evaluate impacts of methyl triclosan. Methyl triclosan represented up to 96% of the total concentrations observed. These results emphasize the importance of monitoring contaminant transformation products, which can be present at higher levels than the parent compound. Environ Toxicol Chem 2023;42:620–627. © 2023 SETAC
An alternative source of probiotics: Water kefir
Authors: Cufaoglu Gizem , Erdinc Ayse Nur 
Published: January 2023

AbstractThe aim of this review was to compile the origin and distribution of water kefir, its production, microbial diversity, fermentation dynamics, health effects, and its place in national/international food legislation. Water kefir is a traditional, slightly carbonated fermented beverage with a lactic acid content of up to 2%. It is obtained by fermenting sugar by kefir grains, which are white or yellowish in color and translucent in appearance. The types of microorganisms in the water kefir differ depending on the sugar, carbon sources, and fermentation conditions. It has a complex microbiota mainly consisting of lactic acid bacteria, acetic acid bacteria, and yeasts. In addition, fermented water kefir contains various metabolites, including sugar, fruit‐derived components, lactic acid, acetic acid, ethanol, carbon dioxide, mannitol, vitamins, and amino acids such as arginine. As a non‐dairy fermented beverage, water kefir emerges as an alternative source of probiotics for individuals with lactose intolerance, people with dairy allergic reactions, and vegan consumers. However, although consumer demands have driven the food industry to increase the production of water kefir, the most common production still relies on the traditional method due to some difficulties in the industrial‐scale production process. On the other hand, more in vitro and in vivo studies should be carried out to reveal the health benefits of water kefir.
Association of genetic variants of CircCHST15 with oral squamous cell carcinoma in the Chinese Han population
Authors: Wang Tianxiao , Zhang Yehao , Wu Jia , Feng Hongjie , Wang Ruixia , Yuan Hua 
Published: January 2023

AbstractBackgroundOral squamous cell carcinoma (OSCC) is the most common cancer in the oral cavity. The relationship between the genetic susceptibility of circCHST15 and OSCC remains unclear.MethodsGenetic variants of circCHST15 were screened using a genotyping analysis from 1044 patients with OSCC and 3199 healthy participants. The circCHST15 expression was detected in 32 pairs of OSCC tissues. The circular RNA quantitative trait locus analysis and the reporter gene assay were performed for verification.ResultsThe circCHST15 expression was upregulated in OSCC (Wilcoxon p < 1e−3). The genotyping analysis screened out 61 loci in circCHST15 associated with the risk of OSCC. After adjustment and annotation, rs28707473 (A > C, odds ratio = 1.21, 95% CI: 1.076–1.361, p = 1.453e−3) was selected. This genetic variation could elevate the circCHST15 expression level possibly by altering the structure of circular RNAs and affecting transcription factor binding.ConclusionsThe results of this study suggested that genetic variants of circCHST15 may contribute to OSCC susceptibility.
Androgenic‐anabolic steroid abuse trend and management: A prospective, cross‐sectional, questionnaire‐based survey
Authors: Al Hashimi Manaf , Farahat Yasser , Kandil Hussein , Al Khalidi Ismail 
Published: January 2023

AbstractBackground and AimsAndrogenic‐anabolic steroid (AAS) abuse is a global health concern, studies revealing an increasing trend of abuse and deleterious effects on reproductive health. Unfortunately, there is no consensus about management pathways due to the lack of specific guidelines.MethodsA prospective study, multicentre, online survey, composed of 30 questions, was conducted to investigate the current trend of AAS abuse and the management followed by practitioners from different specialities dealing with this condition.ResultsA total of 151 respondents were included. The majority were general urologists (68.21%), andrologists (22.51%), and endocrinologists (9.28%). An increasing trend of AAS abuse was noticed by 90.73% of participants mostly in young age populations. Most of AAS abusers were presented with infertility (64.24%) and erectile dysfunction (59.60%), and their investigations showed abnormal semen analysis (77.48%), abnormal hormones (follicle‐stimulating hormone, luteinizing hormone, testosterone, and estradiol) (94.70%), and reduction in testicular size (50.33%). Most of respondents expected: the need of long duration for spontaneous recovery (6–12 months), relapse of AAS abuse in one‐third of patients, less knowledge about the adverse effects (39.74%), and risk of drug dependence (54.30%). Immediate treatment was the most offered plan of management (44.37%) followed by a waiting spontaneous recovery (32.45%), while the remaining would refer the patients to an either endocrinologist or andrologist. The treating physicians did not follow specific guidelines and most of participants (44.44%) reverted to their personal experience in the management.ConclusionsOur study revealed an increasing trend of AAS abuse, deleterious effects of AAS use on reproductive health, and lack of consensuses among the treating physicians regarding the management of related adverse effects. Our study could be considered a call to the scientific bodies to have more studies, establish guidelines for management, and to have better awareness of this serious public health concern.
Fetal glycated albumin levels in offspring of obese women
Authors: Omokhodion Ofure Victoria , Kuti Modupe A. , Odukogbe Akin‐Tunde A. , Omokhodion Folashade Olufunke 
Published: January 2023

AbstractObjectiveTo determine the association between maternal obesity and fetal glycated albumin (GA) levels among pregnant women.MethodsA comparative, cross‐sectional study of 59 consenting, normoglycemic, pregnant women, who met the criteria for maternal obesity, attending the antenatal clinic of the University College Hospital, Nigeria, from June 2019 to December 2019. They were recruited at 36 weeks of gestation, followed up until delivery, and compared with 58 nonobese, normoglycemic pregnant controls. At delivery, blood samples were taken from the mothers and from the umbilical cords of their newborns for serum GA assay. Maternal and newborn variables were recorded, and comparisons were made using χ2 tests, independent t tests, odds ratios, analysis of variance, and Pearson correlates. Statistical significance was set at P < 0.05.ResultsThe odds of elevated newborn GA were 3.21 times higher in obese women compared with nonobese women (P = 0.005) and 5‐min APGAR scores were higher in the newborns of nonobese women (P = 0.039). There was a significant correlation between maternal and neonatal GA for all participants (r = 0.346, P = 0.000).ConclusionThese findings suggest that maternal obesity is associated with elevated fetal GA and low APGAR scores at 5 min in normoglycemic women.
A holistic understanding of information and communication technology for development through context, resilience, and sustainability: Evidence from a local agriculture extension information service in Ethiopia
Authors: Atinaf Muluneh , Anteneh Salehu , Kifle Mesfin 
Published: January 2023

AbstractThere exists isolated work that focuses on context, resilience, and sustainability of information and communication technology for development (ICT4D). However, research on how the three concepts inform and influence one another to better meet the development goals and priorities is limited. More research is required to enhance our holistic understanding of ICT4D interventions in terms of the approaches applied to investigate context, resilience, and sustainability from socio‐technical perspectives. This research explores the link among the triple concepts to fill the above void, drawing from qualitative data collected from multiple but networked stakeholders at a local agriculture extension information service. Data is processed in a way to understand: the context of the local development practices and stakeholders, the gaps in the design of information systems applied to support local development practices, and how local communities remain resilient in their information exchange practices. Building on the existing knowledge of context, resilience, and sustainability in ICT4D the research shows how the context of the local development practices inform resilience and in turn how resilience enables the continued operation of the local development practices (context), and how context and resilience lead to sustainable ICT4D interventions. The research contributes to theory by showing how the social and technical resources distributed in the social system and the stakeholders' networks can be unlocked to conceptualize the links between the triple concepts. It informs practitioners on how they can develop a holistic understanding of the socio‐technical contexts of local developments and the socio‐technical factors to design resilient ICT4D that will lead to sustainable digital interventions.
Evaluation of perceived stress and resilience among postgraduate academic‐affiliated pharmaceutical industry fellows
Authors: Strong Delaney M. , Amin Sana , Caputo Joseph , Heck Sheehan Amy , Seyss Carolyn , Mistry Amee , Miller Monica L. 
Published: January 2023

AbstractIntroductionIt has been determined that pharmacy residents experience high levels of perceived stress; however, the extent of stress perceived by pharmaceutical industry fellows has not been quantified. This study examined the level of perceived stress academic‐affiliated postgraduate pharmaceutical industry fellows experience, along with identification of what types of stressors they experience, possible factors associated with higher levels of perceived stress, and the state of resilience skills in this population.MethodsA multi‐item survey instrument was created to collect information about fellow demographics, perceived stress using the Perceived Stress Scale (PSS‐10), major stressors, and resilience skills using the brief Connor‐Davidson Resilience Scale (CD‐RISC‐10). Postgraduate year one (PGY1) and postgraduate year two (PGY2) industry‐affiliated pharmaceutical fellows at programs with academic collaborations through Rutgers University Ernest Mario School of Pharmacy, Massachusetts College of Pharmacy and Health Sciences (MCPHS), and Purdue University College of Pharmacy were invited to complete the anonymous survey instrument from March to May 2022.ResultsA total of 83 complete responses were received, for a response rate of 21.4%. The mean PSS‐10 score for fellows was 14.8 (SD, 5.7). For resilience, the mean CD‐RISC‐10 score was 29.0 (SD, 5.2). No relationships were found between fellow demographics, perceived stress, and resilience skills. Fellows reported experiencing stress caused by work role/responsibilities (72.3%), personal/physical stressors (68.7%), work environment (37.3%), and home environment (28.9%) in the past month. The top reported coping strategies were exercising/sports (65.1%), spending time with family/friends (48.2%), and watching television (33.7%).ConclusionAcademic‐affiliated pharmaceutical industry fellows reported a moderate level of perceived stress and lower resilience levels than the general population. Results from this research will be used to develop a customized wellness initiative to support pharmaceutical industry fellows.
Microlesion healing dynamics in in vivo porcine skin after treatment with 1064 nm picosecond‐domain Nd:YAG laser
Authors: Baleisis Justinas , Rudys Romualdas 
Published: January 2023

AbstractThe fractionated picosecond laser produces microscopic lesions in the epidermis and dermis due to laser‐induced optical breakdown (LIOB). There have been multiple histological reports, but the present literature lacks detailed in vivo studies after treatment with high‐power laser systems. Our study aimed to characterize the healing patterns of microlesions induced with 150 ps duration 1064 nm MLA‐type picosecond laser. The induced picosecond laser‐tissue reactions with pulse energy of 50–250 mJ and different treatment modes were observed in in vivo porcine skin model over 10 days after the laser procedure. A macroscopic evaluation was combined with microscopic histological analysis to observe the healing dynamics of laser‐induced microlesions. Superficial, intraepidermal cavitation bubbles were induced using microbeam fluence of 4–20 J/cm2. Skin irritation scores positively correlated with pulse energy and dose. Our findings demonstrate that dose and pulse energy had a direct impact on epidermal thickness and lesions healing dynamics.
Density functional theory study on sensing properties of g‐C3N4 sheet to atmospheric gasses: Role of zigzag and armchair edges
Authors: Nachimuthu Santhanamoorthi , Kuo Yi‐Hui , Khanh Dang Hoai , Zhu Zhan‐Jun , Jiang Jyh‐Chiang 
Published: January 2023

AbstractThe ability of the polymer‐based graphitic carbon nitride (g‐C3N4) as a gas sensor toward NO, NO2, CO, CO2, SO2, SO3, and O2 gasses is assessed using density functional theory (DFT) calculations in terms of energetic and electronic transport characteristics. In particular, this study is aimed to explore the role of zigzag and armchair edges of the g‐C3N4 sheet on sensing performances. The electronic properties of adsorption systems, such as Bader charge analysis, band gaps, work function, and density of states (DOS), are used to understand the interaction between the adsorbed gas molecules and the g‐C3N4 sheet. Our calculated results indicate that SOx (SO3 and SO2) gasses have higher adsorption energies on the g‐C3N4 sheet than other gasses. Furthermore, the transport properties, such as current–voltage (I‐V) and resistance‐voltage (R‐V) curves along the zigzag and armchair directions are calculated using the non‐equilibrium Green's function (NEGF) method to understand the performance of the g‐C3N4 sheet as a prominent conductive/resistive sensor. The I‐V/R‐V results indicate that the zigzag g‐C3N4 sheet has excellent sensing ability toward SOx gasses at low applied voltages. However, the presence of H2O degrades the sensing performance of the armchair g‐C3N4 sheet. Theoretical recovery time has also been calculated to evaluate the reusability of g‐C3N4 sheet‐based gas sensors. Our results reveal that the zigzag g‐C3N4 sheet‐based sensing device has a remarkably high sensitivity (>300%) and selectivity toward SOx gasses and has the potential to work in a complex environment.
The maximum number of columns in E(s2) $\,E({s}^{2})$‐optimal supersaturated designs with 16 rows and smax=4 ${s}_{{\rm{\max }}}=4$ is 60
Authors: Morales Luis B. 
Published: January 2023

AbstractWe show that the maximum number of columns in ‐optimal supersaturated designs (SSDs) with 16 rows and is 60 by showing that there exists no resolvable 2‐(16, 8, 35) design such that any two blocks from different parallel classes intersect in 3, 5, or 4 points. This is accomplished by an exhaustive computer search that uses the parallel class intersection pattern method to reduce the search space. We also classify all nonisomorphic ‐optimal 5‐circulant SSDs with 16 rows and .
Measurement invariance and psychometric properties of the CCAPS‐62 and ‐34 for international student clients
Authors: Bartholomew Theodore T. , Keum Brian T. , Lockard Allison J. , Pérez‐Rojas Andres E. , Robbins Krista A. , Zhao Fanghui 
Published: January 2023

AbstractObjectivesInternational students attending universities in the United States may encounter psychological distress related to their adjustment and experiences studying in a new context and seek services from university counseling centers. Many centers use the Counseling Center Assessment of Psychological Symptoms (CCAPS) to measure psychological distress in college counseling centers. However, this scale has not been tested for measurement invariance with international students. Our purpose was to explore the measurement invariance of the CCAPS‐62 and ‐34 for international students.MethodsWe tested measurement invariance for both versions of the scale using data from over 107,000 university students in psychotherapy at university counseling centers. We also examined construct validity and internal consistency.ResultsInvariance testing indicated the measurement models of the CCAPS‐62 and ‐34 are equivalent between both groups.ConclusionThese findings are discussed in light of utilizing this widely‐used scale in United States university/college counseling centers with international student clients.
Supplemental calcium increases hatch rate but not hatchling mass of chick embryos in shell‐less culture
Authors: Dunn Bruce E. 
Published: January 2023

AbstractA method is described for culturing 64–70 h‐old chicken embryos and egg contents outside of the eggshell through to hatching. Cultured egg contents were suspended in polymethylpentene kitchen wrap (F.O.R. wrap; Riken Fabro) supported in polyvinyl chloride tripods. Tripods were incubated in Plexiglas environmental chambers which were rocked automatically through an angle of ±20°. The concentration of CO2 was maintained at 2% throughout incubation, while that of O2 was increased from ambient to 50%, and relative humidity was decreased from 90%–92% to 83%–84% at incubation Day 9. Cultured embryos not supplemented with calcium did not hatch. The Hatch rate increased when supplemental calcium L‐lactate hydrate was increased between 250 and 350 mg. A maximal hatch rate of 54.8% was achieved when cultures were supplemented with 350 mg of calcium L‐lactate hydrate and 3.5 ml of sterile water. Adding 400 or 450 mg of calcium L‐lactate hydrate did not increase the hatch rate further. The mass of cultured hatchlings (including the retracted yolk) and yolk‐free carcass wet and dry mass and length of the right third toe were significantly less than the corresponding parameters observed in hatchlings in ovo. No statistically significant differences in hatchling mass, yolk‐free carcass wet or dry mass, or length of the right third toe were noted among cultured hatchlings supplemented with 250–450 mg of calcium L‐lactate hydrate. Failure to completely absorb albumen was the most common abnormality observed in cultures which failed to hatch. The present technique allows a unique approach to study the physiology of the developing chicken embryo.