bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perinatal folate levels do not influence tumor latency or multiplicity in a model of NF1 associated plexiform-like neurofibromas
Authors: Marley Andrew R. , Williams Kyle B. , Tibbitts Justin , Moertel Christopher L. , Johnson Kimberly J. , Linden Michael A. , Largaespada David A. , Marcotte Erin L. 
Published: January 2023

AbstractObjectiveIn epidemiological and experimental research, high folic acid intake has been demonstrated to accelerate tumor development among populations with genetic and/or molecular susceptibility to cancer. Neurofibromatosis type 1 (NF1) is a common autosomal dominant disorder predisposing affected individuals to tumorigenesis, including benign plexiform neurofibromas; however, understanding of factors associated with tumor risk in NF1 patients is limited. Therefore, we investigated whether pregestational folic acid intake modified plexiform-like peripheral nerve sheath tumor risk in a transgenic NF1 murine model.ResultsWe observed no significant differences in overall survival according to folate group. Relative to controls (180 days), median survival did not statistically differ in deficient (174 days,P=0.56) or supplemented (177 days,P=0.13) folate groups. Dietary folate intake was positively associated with RBC folate levels at weaning, (P=0.023, 0.0096, and 0.0006 for deficient vs. control, control vs. supplemented, and deficient vs. supplemented groups, respectively). Dorsal root ganglia (DRG), brachial plexi, and sciatic nerves were assessed according to folate group. Mice in the folate deficient group had significantly more enlarged DRG relative to controls (P=0.044), but no other groups statistically differed. No significant differences for brachial plexi or sciatic nerve enlargement were observed according to folate status.
The TNFΔAREmouse as a model of intestinal fibrosis
Authors: Steiner Calen A , Koch Samuel D , Evanoff Tamara , Welch Nichole , Kostelecky Rachael , Callahan Rosemary , Murphy Emily M , Hall Caroline H T , Lu Sizhao , Weiser-Evans Mary CM , Cartwright Ian M , Colgan Sean P 
Published: January 2023

ABSTRACTBackground & AimsCrohn’s disease (CD) is a highly morbid chronic inflammatory disease. The majority of CD patients also develop fibrostenosing complications. Despite this, there are no medical therapies for intestinal fibrosis. This is in part due to lack of high-fidelity biomimetic models to enhance understanding and drug development. There is a need to developin vivomodels of inflammatory bowel disease-related intestinal fibrosis. We sought to determine if the TNFΔAREmouse, a model of ileal inflammation, may also develop intestinal fibrosis.MethodsSeveral clinically relevant outcomes were studied including features of structural fibrosis, histological fibrosis, and gene expression. These include the use of a luminal casting technique we developed, traditional histological outcomes, use of second harmonic imaging, and quantitative PCR. These features were studied in aged TNFΔAREmice as well as in cohorts of numerous ages.ResultsAt ages of 24+ weeks, TNFΔAREmice develop structural, histological, and genetic changes of ileal fibrosis. Genetic expression profiles have changes as early as six weeks, followed by histological changes occurring as early as 14-15 weeks, and overt structural fibrosis delayed until after 24 weeks.DiscussionThe TNFΔAREmouse is a viable and highly tractable model of intestinal fibrosis. This model and the techniques employed can be leveraged for both mechanistic studies and therapeutic development for the treatment of intestinal fibrosis.
Active compensation for changes inTDH3expression mediated by direct regulators ofTDH3inSaccharomyces cerevisiae
Authors: Vande Zande Pétra , Wittkopp Patricia J. 
Published: January 2023

AbstractGenetic networks are surprisingly robust to perturbations caused by new mutations. This robustness is conferred in part by compensation for loss of a gene’s activity by genes with overlapping functions, such as paralogs. Compensation occurs passively when the normal activity of one paralog can compensate for the loss of the other, or actively when a change in one paralog’s expression, localization, or activity is required to compensate for loss of the other. The mechanisms of active compensation remain poorly understood in most cases. Here we investigate active compensation for the loss or reduction in expression of theSaccharomyces cerevisiaegeneTDH3by its paralogsTDH1andTDH2. TDH1andTDH2are upregulated in a dose-dependent manner in response to reductions inTDH3by a mechanism requiring the shared transcriptional regulators Gcr1p and Rap1p. Other glycolytic genes regulated by Rap1p and Gcr1p show changes in expression similar toTDH2, suggesting that the active compensation byTDH3paralogs is part of a broader homeostatic response mediated by shared transcriptional regulators.
Draft Genome Sequence ofPedococcussp. Strain 5OH_020 Isolated from California Grassland Soil
Authors: Green Myka A. , Alvarez-Aponte Zoila I. , Trotter Valentine V. , Taga Michiko E. 
Published: January 2023

AbstractThe draft genome sequence of the soil bacteriumPedococcussp. 5OH_020, isolated on a natural vitamin B12analog, contains 4.4 Mbp with 4,108 protein-coding genes. Its genome encodes B12-dependent enzymes including methionine synthase and class II ribonucleotide reductase. Taxonomic analysis suggests it is a novel species within the genusPedococcus.
Cuticular profiling of insecticide resistantAedes aegypti
Authors: Jacobs Ella , Chrissian Christine , Rankin-Turner Stephanie , Wear Maggie , Camacho Emma , Scott Jeff G. , Broderick Nichole A. , McMeniman Conor J. , Stark Ruth E. , Casadevall Arturo 
Published: January 2023

AbstractInsecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contributing mechanisms. Contact-based insecticides are absorbed through the insect cuticle, which is comprised mainly of chitin polysaccharides, cuticular proteins, hydrocarbons, and phenolic biopolymers sclerotin and melanin. Cuticle interface alterations can slow or prevent insecticide penetration in a phenomenon referred to as cuticular resistance. Cuticular resistance characterization of the yellow fever mosquito,Aedes aegypti, is lacking. In the current study, we utilized solid-state Nuclear Magnetic Resonance (ssNMR) spectroscopy, gas chromatography/mass spectrometry (GC-MS), and transmission electron microscopy (TEM) to gain insights into the cuticle composition of congenic cytochrome P450 monooxygenase insecticide resistant and susceptibleAe. aegypti. No differences in cuticular hydrocarbon content or phenolic biopolymer deposition were found. In contrast, we observed cuticle thickness of insecticide resistantAe. aegyptiincreased over time and exhibited higher polysaccharide abundance. Moreover, we found these local cuticular changes correlated with global metabolic differences in the whole mosquito, suggesting the existence of novel cuticular resistance mechanisms in this major disease vector.
Biophysical modeling with variational autoencoders for bimodal, single-cell RNA sequencing data
Authors: Carilli Maria , Gorin Gennady , Choi Yongin , Chari Tara , Pachter Lior 
Published: January 2023

AbstractWe motivate and presentbiVI, which combines the variational autoencoder framework ofscVIwith biophysically motivated, bivariate models for nascent and mature RNA distributions. While previous approaches to integrate bimodal data via the variational autoencoder framework ignore the causal relationship between measurements,biVImodels the biophysical processes that give rise to observations. We demonstrate through simulated benchmarking thatbiVIcaptures cell type structure in a low-dimensional space and accurately recapitulates parameter values and copy number distributions. On biological data,biVIprovides a scalable route for identifying the biophysical mechanisms underlying gene expression. This analytical approach outlines a generalizable strateg for treating multimodal datasets generated by high-throughput, single-cell genomic assays.
Coupling During Collective Cell Migration is Controlled by a Vinculin Mechanochemical Switch
Authors: Shoyer T. Curtis , Gates Evan M. , Cabe Jolene I. , Conway Daniel E. , Hoffman Brenton D. 
Published: January 2023

ABSTRACTCollective cell migration (CCM) plays important roles in development, physiological, and pathological processes. A key feature of CCM is the dynamic mechanical coupling between cells, which enables both long-range coordination and local rearrangements. This coupling requires the ability of cell adhesions to adapt to forces. Recent efforts have identified key proteins and implicated cellular-scale mechanical properties, but how key proteins give rise to these larger-scale mechanical processes is unclear. Using force-sensitive biosensors, cell migration assays, and molecular clutch models, we sought a molecular understanding of adhesion strengthening that could bridge this gap. We found that the mechanical linker protein vinculin bears substantial loads at AJs, FAs, and in the cytoplasm during epithelial sheet migration, and we identified a switch-like residue on vinculin that regulates its conformation and loading at the AJs during CCM. In vinculin KO-rescue, this switch jointly controlled the speed and coupling length-scale of CCM, which suggested changes in adhesion-based friction. To test this, we developed molecularly detailed friction clutch models of the FA and AJ. They show that open, loaded vinculin increases friction in adhesive structures, with larger affects observed in AJs. Thus, this work elucidates how load-bearing linker proteins can be regulated to alter mechanical properties of cells and enable rapid tuning of mechanical coupling in CCM.
The exon junction complex component EIF4A3 is essential for mouse and human cortical progenitor mitosis and neurogenesis
Authors: Lupan Bianca M. , Solecki Rachel A. , Musso Camila Manso , Alsina Fernando C. , Silver Debra L. 
Published: January 2023

AbstractMutations in components of the exon junction complex (EJC) are associated with neurodevelopment and disease. In particular, reduced levels of the RNA helicaseEIF4A3cause Richieri-Costa-Pereira Syndrome (RCPS) and CNVs are linked to intellectual disability. Consistent with this,Eif4a3haploinsufficient mice are microcephalic. Altogether, this implicates EIF4A3 in cortical development; however, the underlying mechanisms are poorly understood. Here, we use mouse and human models to demonstrate that EIF4A3 promotes cortical development by controlling progenitor mitosis, cell fate, and survival.Eif4a3haploinsufficiency in mice causes extensive cell death and impairs neurogenesis. UsingEif4a3;p53compound mice, we show that apoptosis is most impactful for early neurogenesis, while additional p53-independent mechanisms contribute to later stages. Live imaging of mouse and human neural progenitors revealsEif4a3controls mitosis length, which influences progeny fate and viability. These phenotypes are conserved as cortical organoids derived from RCPS iPSCs exhibit aberrant neurogenesis. Finally, using rescue experiments we show that EIF4A3 controls neuron generation via the EJC. Altogether, our study demonstrates that EIF4A3 mediates neurogenesis by controlling mitosis duration and cell survival, implicating new mechanisms underlying EJC-mediated disorders.Summary statementThis study shows that EIF4A3 mediates neurogenesis by controlling mitosis duration in both mouse and human neural progenitors, implicating new mechanisms underlying neurodevelopmental disorders.
Commodifying participation through choreographed engagement: the Taylor Swift case
Authors: Ryan Bengtsson Linda , Edlom Jessica 
Published: January 2023

PurposeThis article examines the ways in which the popular music industry markets artists through integrated transmedia marketing campaigns. These campaigns unfold across multiple media and create multiple pathways for audience engagement, particularly fan engagement, across social media platforms. The purpose is to further theorise the relationship between artists, the music industry and audiences.Design/methodology/approachThe study used digital ethnography to scrutinise the activities within a contemporary music transmedia marketing campaign, focusing on the release of Taylor Swift's album Reputation as an illustrative case.FindingsThe study demonstrates how strategically curated activities encompass platforms' affordances and industry events by making use of fan engagement across social media platforms and streaming services. Fans shift through platforms, as well as across digital and physical spaces, through defined marketing activities at specific times. This article proposes the concept of choreographed engagement to specifically address the ways in which the temporal and spatial aspects of social media marketing are used at the intersection of platform logic, algorithm economy and fan engagement to reach wider audiences.Originality/valueBy proposing the concept of choreographed engagement, the authors bridge the gap between fan practices and marketing practices, providing insight into how commodification of fan engagement is utilised spatially and temporally within the contemporary platform economy. Choreographed engagement constitutes a significant aspect of strategic communication and marketing. The term expands the vocabulary used in the debate on the commodification of artistic work, and audience engagement in the platform era.
The empirical relationship between transformational leadership and job attitudes: mediating role of psychological capital – a study of healthcare in India
Authors: Shah Tanveer Ahmad , Parray Zahoor Ahmad , Islam Shahbaz ul 
Published: January 2023

PurposeThe goal of this research is to investigate the association between transformational leadership style, psychological capital and job attitude (job satisfaction and organization commitment). Transformational leadership's effects on these job attitudes were also analysed in terms of the mediating effects of psychological capital.Design/methodology/approachThe authors received 296 authentic questionnaires from the para-medical staff working in different public and private healthcare institutes in Jammu and Kashmir, India, and these were then analysed by using SEM with SPSS 25.0 and AMOS 19.FindingsResults implied that transformational leadership increased the levels of employees' job attitudes. The results further displayed that transformational leadership and psychological capital in the workplace are positively associated. The results also showed that psychological capital was positively linked with job attitudes. Moreover, psychological capital serves as a mediating construct between transformational leadership and job attitudes.Practical implicationsThe outcomes of this research will help in comprehending the significance of transformational leadership and psychological capital. Further, these research findings affirm the effectiveness of transformational leadership and psychological capital in forecasting positive job attitudes in the Indian work context. The administrators and policymakers in the healthcare sector can implement these concepts to reduce negative job outcomes.Originality/valueThis study expanded on Deciet al.’s (2017) existing self-determination theory model by incorporating leadership style and psychological capital as workplace context and autonomous intrinsic factors, respectively, into the self-determination theory model to study work behaviours of job satisfaction and commitment. This study contributes to existing self-determination theory knowledge by proposing and testing psychological capital as a mechanism for determining the effect of transformational leadership on job attitudes (job satisfaction and organizational commitment).
Revisiting the finance-growth nexus in Hong Kong: fresh insights from nonparametric analysis
Authors: Almassri Hamza , Ozdeser Huseyin , Saliminezhad Andisheh 
Published: January 2023

PurposeSince financial sector plays a critical economic role in Hong Kong, the current research aims to comprehensively analyze the association between financial development and economic growth in the country to draw correct conclusions about the impact that financial sector's development has on the growth of the economy. This requires both using of more comprehensive data that includes all or nearly all elements of the country's financial sector and utilizing advanced econometrics techniques to provide more reliable evidence based on the findings. In the study, both issues have been addressed more academically to aid the relevant authorities better.Design/methodology/approachThis study empirically examines the financial development-economic growth nexus in Hong Kong employing data covering 1980–2019. The quantile-on-quantile (QQ) approach of Sim and Zhou (2015) is utilized to investigate certain subtle aspects of the association linking financial development and economic growth. In addition, the authors benefit from applying the nonlinear Granger causality test of Diks and Panchenko (2006) to assess the variables' nexus in a nonlinear manner.FindingsIn contrast to the evidence of a unidirectional linkage documented in many related studies, the empirical findings suggest that a bi-directional relationship exists between financial development and economic growth for Hong Kong. This is a helpful input for the relevant policymakers and implies that they can set appropriate policies and regulations to balance financial development and economic growth in this country.Originality/valueThe originality of this study can be divided into two parts. Methodologically, unlike past studies that utilized mostly linear and parametric methods, the paper contributes to the literature by applying the more robust nonparametric and nonlinear methodologies. Theoretically, most researchers have used various financial development indicators, which led to very different conclusions. Therefore, this study attempts to resolve this deficiency in the literature by using a more comprehensive index for financial development.
Becoming a global library at the local: internationalization activities and practices of Philippine academic libraries
Authors: Ramos-Eclevia Marian 
Published: January 2023

PurposeThis study aims to examine the perceived role of libraries and contributions to the internationalization of higher education and identify the available library services for international students and faculty.Design/methodology/approachThis descriptive research used a survey method to study the different activities and practices of the internationalization of academic libraries.FindingsThe results of this study reveal that academic libraries support the teaching, learning and research needs of international students and faculty members, provide library instruction for international students to acquire information literacy skills and promote the rationale and goals of the institution's internationalization activities. Many respondents are engaged in staff development with internationalization dimensions and international library benchmarking and research paper presentation at international conferences with institutional support.Practical implicationsThe results of this study could benefit the profession and library managers by deepening the managers' understanding of the role of libraries in the internationalization of Philippine higher education.Originality/valueThis study will be of interest and value to library and school administrators interested in developing or expanding internationalization activities that positively respond to the redefinition of the role of libraries and librarians in globalization.
Changes in fasting blood glucose status and incidence of cardiovascular disease: The China‐PAR project
Authors: Tong Ye , Liu Fangchao , Huang Keyong , Li Jianxin , Yang Xueli , Chen Jichun , Liu Xiaoqing , Cao Jie , Chen Shufeng , Yu Ling , Zhao Yingxin , Wu Xianping , Zhao Liancheng , Li Ying , Hu Dongsheng , Huang Jianfeng , Lu Xiangfeng , Shen Chong , Gu Dongfeng 
Published: January 2023

AbstractBackgroundThe effect of long‐standing prediabetes or its transition on incident cardiovascular disease (CVD) is unclear. This study aimed to evaluate the association of changes in fasting blood glucose (FBG) status with the risk of developing CVD.MethodsThis research included 12 145 Chinese adults aged 35–74 years and free from diabetes mellitus (DM) at baseline. Study participants were cross‐classified into six categories according to glucose at the first (1998–2001) and the second visit after 8 years: normal fasting glucose (NFG; 50–99 mg/dl), impaired FBG (IFG; 100–125 mg/dl), and DM. Cox proportional hazard regression model was used to estimate the hazard ratio (HR) and 95% confidence interval (CI) for CVD associated with transition of glucose status.ResultsDuring a median follow‐up of 5.5 years, 373 incident CVD cases occurred. Compared with participants remaining persistent NFG, a higher risk of developing CVD was identified among those remaining persistent IFG, progressing to DM from NFG or from IFG, with the multivariate‐adjusted HR (95% CI) of 1.792 (1.141, 2.816), 1.723 (1.122, 2.645) and 1.946 (1.120, 3.381), respectively. Furthermore, when stratified by glucose status at baseline, persistent IFG and progression from IFG to DM still increased CVD risk in comparison with reversion from IFG to NFG, with the multivariate‐adjusted HR (95% CI) of 1.594 (1.003, 2.532) and 1.913 (1.080, 3.389).ConclusionsParticipants with long‐standing IFG and progressing to DM had a higher risk of developing CVD. Further well‐designed studies are warranted to assess the association of other phenotypes or prediabetes duration with CVD.
Cast away on Mindoro island: lack of space limits population growth of the endangered tamaraw
Authors: Bonenfant C. , Rutschmann A. , Burton J. , Boyles R. , García F. , Tilker A. , Schütz E. 
Published: January 2023

AbstractEndangered species, despite often living at low population densities, may undergo unexpected density‐dependent feedbacks in the case of successful recovery or marked reduction in range. Because density‐dependence dynamics can increase risk of extinction, these effects can hamper conservation efforts. In this study, we analyze the dynamics of the largest population of the tamaraw Bubalus mindorensis, a critically endangered ungulate species endemic to Mindoro island, Philippines. The population is located within a <3000 ha area in Mounts Iglit‐Baco Natural Park, with limited expansion possibilities. We took advantage of a 22 year time series of tamaraw counts to estimate annual population growth rate and possible density‐dependence, accounting for sampling errors in the counts. The tamaraw population has been increasing at an average rate of +5% per year, as would be expected given its protected status by law. Population growth showed strong spatial structuring, with a population growth close to +10% in the core area of protection, and a reduction of abundance of −5% at the periphery of its range, inside the protected area. This range constriction is concerning because our best population dynamics model suggests significant negative density‐dependence (Bayes factor = 0.9). The contraction of tamaraw range is likely caused by anthropogenic pressures forcing the species to live at relatively high densities in the core zone where protection is most effective, creating source‐sink dynamics. Our study highlights the fact that, despite the continuous population growth over the last two decades, the long‐term viability of the Mounts Iglit‐Baco Natural Park tamaraw population remains uncertain.