bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibody and antibody fragments Site Specific Conjugation using New Q-tag Substrate of Bacterial Transglutaminase.
Authors: Alaoui Said El , Sivado Eva , Alaoui Meddy El , Jallas Anne-Catherine , Mebarki Lamia , Dyson Michael R. , Perez Franck , Valsesia-Wittmann Sandrine 
Published: September 2023

Abstract
During the last few years Antibody Drug Conjugates (ADCs) have become one of the most active and very promising therapeutic weapons. Lessons learned from the traditional chemical conjugations (via lysine or cysteine residues of the antibodies) and the clinical studies of the developed ADCs have recently paved the way to the improvement of the conjugation technologies. Use of site-specific conjugation is considered as the promising path for improving the design and development of homogeneous ADCs with controlled Drug-Antibody ratio (DAR). Moreover, some of these conjugations can be applied to antibody fragments such as Fab, scfv and VHH for which random and chemical conjugation showed significant limitations. In this study, we identified a novel small peptide substrate (Q-tag) with high affinity and specificity of bacterial transglutaminase which can be genetically fused to different formats of antibodies of interest for the development of enzymatic site-specific conjugation we named “CovIsolink” platform. We describe the synthesis of chemically defined drugs conjugation in which the site and stoichiometry of conjugation are controlled using a genetically encoded Q-tag peptide with specific amino acids which serves as a substrate of bacterial transglutaminase. This approach has enabled the generation of homogeneous conjugates with DAR 1,7 for full IgG and 0,8 drug ratio for Fab, scfv and VHH antibody fragments without the presence of significant amounts of unconjugated antibody and fragments. As a proof of concept, Q-tagged anti Her-2 (human IgG1 (Trastuzumab) and the corresponding fragments (Fab, scfv and VHH) were engineered and conjugated with different aminated-payloads. The corresponding Cov-ADCs were evaluated in series of in vitro and in vivo assays, demonstrating similar tumor cell killing potency as Trastuzumab emtansine (Kadcyla®) even with lower drug-to-antibody ratio (DAR).
The decreasing transport of the Deep Western Boundary Current in the subpolar Atlantic
Authors: Koman Gregory , Bower Amy , Holliday N. Penny , Furey Heather , Fu Yao , Biló Tiago 
Published: September 2023

Abstract

The Deep Western Boundary Current (DWBC) – the primary component of the lower limb of the Atlantic Meridional Overturning Circulation – flows along the eastern flank of Greenland from a combination of Denmark Strait Overflow Water and Iceland Scotland Overflow Water. The Overturning in the Subpolar North Atlantic Program (OSNAP) has continuously measured the DWBC since 2014 using current meters, temperature/salinity sensors, and acoustic doppler current profilers. This mooring array located near Cape Farewell also incorporates data from the Ocean Observatories Initiative’s Global Irminger Sea Array to create the longest continuous observations of the DWBC closest to where Iceland Scotland Overflow Water and Denmark Strait Overflow water first merge. This study reveals that the DWBC has decreased by 26% over the first six years of OSNAP observations primarily due to a thinning of the traditionally defined DWBC layer (σθ > 27.8 kg m-3) due to a known freshening signal moving through the subpolar region. Despite this decrease, the Atlantic Meridional Overturning Circulation as calculated by OSNAP has remained relatively steady over the same period. Ultimately, the reason for this difference is due to the methods used to define these two circulations. Finding such notably different trends for two seemingly dependent circulations raises the question of how to best define these transports.
Skin-inspired all-natural ionic biogel
Authors: Ying Yibin , Lan Lingyi , Ping Jianfeng , Li Huiyan , Wang Chengjun , Li Guang , Song Jizhou 
Published: September 2023

Abstract
Natural material-based hydrogels are considered ideal candidates for building robust bio-interfaces due to their environmentally sustainable nature and biocompatibility. However, these hydrogels often suffer from limitations such as weak mechanical strength, low water resistance, and poor ionic conductivity. Here, inspired by the role of natural moisturizing factor (NMF) in skin, we propose a simple yet versatile strategy to fabricate all-natural ionic biogel that is highly resilient, ionically conductive, anti-dehydrated, and fully degradable, without requiring any chemical modification. A well-balanced combination of gelatin and NMF gives rise to a significant enhancement in the mechanical strength, ionic conductivity, and water retention capacity of the biogel than pure gelatin hydrogel. The biogel manifests temperature-controlled reversible fluid-gel transition properties due to the triple-helix junctions of gelatin, which enables in situ gelation on various substrates, resulting in conformal contact and dynamic compliance with curved surfaces. Due to its salutary properties, the biogel can serve as an effective and biocompatible interface for high-quality and long-term electrophysiological signal recording. These findings provide a general and scalable strategy for the design of natural material-based hydrogels with tailored functionalities to meet diverse application needs.
An 11-GRG Predicting Model for UCEC Prognosis
Authors: Su Hailan , Qiao Zhenguo 
Published: September 2023

Abstract
Background Endometrial cancer (EC) is indeed one of the most prevalent gynecological malignancies. Further research is required to gain insights into the molecular pathways involved in EC tumorigenesis and to develop more accurate prognostic prediction methods.Method Data from the Cancer Genome Atlas( TCGA) database was used and validated using two GEO datasets, specifically GSE6008 and GSE17025. Various bioinformatics analyses were performed, including the Least Absolute Shrinkage and Selection Operator regression (lasso) regression, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis Gene Set Variation Analysis (GSVA), gene set enrichment analysis (GSEA), protein-protein interaction (PPI) network analysis, COX regression, calibration curves, and decision curve analysis (DCA). These analyses aimed to explore the associations and functions of GRGs in EC development, as well as develop a predictive model for prognosis assessment.Results There were 11 GRGs identified as significantly associated with EC by the Lasso regression, GSEA, and PPI. The further analysis identifies 61 miRNA molecules, 24 RBPs, 139 TFs, and 21 potential drugs or molecular compounds that might have links to these 11 key genes. These 11 GRGs were made into a Cox regression prediction model, among which the PGK2 shows significantly higher utility than other variables. Calibration analysis and DCA indicate that the clinical predictive performance of this 11-GRGs multivariate Cox regression model is highest at 5 years, followed by 3 years and 1 year. There were 6 genes (GPI, HK1, NUP188, PDHA1, PDHA2, PGK2) that exhibited higher predictive accuracy in time-dependent ROC curves.Conclusion The highly enriched GRGs that have been identified might provide a new understanding of the development of EC and its treatment. Moreover, the 11-GRGs model that has been constructed holds significant clinical implications for evaluating prognosis and providing specific therapy guidance to individuals with EC. Among the variables in the 11-GRG model, PGK2 demonstrates notably higher usefulness, highlighting its potential clinical value in EC.
Fabricating a hepatocyte-targeted fullerene derivative as a new lipid droplet regulator
Authors: Li Hongliang , Zou Toujun , Wan Juan , Liao Rufang , Qu Weiyi , Yang Jinjie , Zhang Xiang , Bai Lan , Zhou Junjie , Tian Tian , Tang Qinchao , Zhang Yufeng , Zhao Chong , Yao Xinxin , Cai Zhiwei , Tian Song , Jiang Jingwei , Cheng Xu , Hu Yufeng , Yang Hailong , Zhang Ejuan , Zhang Xiao-Jing , Xu Haibo , She Zhi-Gang 
Published: September 2023

Abstract
Lipid droplets (LDs) are crucial organelles in organisms and hold a critical function in modulating intracellular lipid metabolism and bioenergetic regulation. The homeostasis of LDs directly participates in the onset and progression of non-alcoholic steatohepatitis (NASH) and other metabolic disorders. However, no pharmacological approaches have been developed to target LDs-related dysfunction for metabolic diseases. Here, we systemically screened biocompatible nanoparticles for anti-LDs formation capacities, and identified a carboxyl fullerene derivative, named four malonate groups-substituted C70 fullerene (QF70), as the most potent lead. Notably, QF70 could be directly internalized into hepatocytes and facilitate lysosomal degradation of perilipin-2 (PLIN2), the key molecule in LDs formation and stability. More importantly, oral administration of QF70 robustly blocked both diet- and Leptin deficiency-induced NASH development with significant improvement in obesity and insulin resistance. We further validated the clinical application potential of QF70 in NASH related metabolic disorders in a non-primate model. To our knowledge, this is the first-in-class demonstration for a nanoparticle based agent as a LDs homeostasis-targeted therapeutic to treat metabolic diseases.
May brood desertion be ruled by partner parenting capability in a polygamous songbird? An experimental study
Authors: Zheng Jia , Wang Hui , Jiang Jiayao , Versteegh Maaike A. , Zhou Zhuoya , Zhang Zhengwang , Chen De , Komdeur Jan 
Published: September 2023

Abstract

Parents confront multiple aspects of offspring demands and need to coordinate different parental care tasks. Biparental care is considered to evolve under circumstances where one parent is not competent for all tasks and cannot efficiently raise offspring. However, this hypothesis is difficult to test, as uniparental and biparental care rarely coexist. Chinese penduline tits (Remiz consobrinus) provide such a system where both parental care types occur. Here, we experimentally investigated whether parents in biparental nests is less capable of caring than parents in uniparental nests. We monitored parenting efforts at (1) naturally uniparental and biparental nests and (2) biparental nests before and during the temporary removal of a parent. We found that total feeding frequency and brooding duration were similar for natural uniparental and biparental nests. Feeding frequency, but not brooding duration, contributed significantly to nestling mass. In line with this, parental removal revealed that the remaining parents at biparental nests fully compensated for the partner’s feeding absence but not for brooding duration. This reflects that the manipulated parents are confronted with a trade-off between feeding and brooding and were selected to invest in the more influential one. However, such a trade-off was not observed in parents of natural uniparental care nests. The different capabilities of a parent independently coordinating feeding and brooding tasks indicated that parents from biparental and uniparental nests experienced different environmental conditions.
Responses of Marine Trophic Levels to the Combined Effects of Ocean Acidification and Warming
Authors: Hollander Johan , Hu Nan , Bourdeau Paul 
Published: September 2023

Abstract
Marine organisms are simultaneously exposed to anthropogenic stressors associated with ocean acidification and warming with likely interactive effects. Although new research has started to uncover how marine primary producers, herbivores, and predators are responding to climate change, we still do not have a comprehensive understanding of general patterns across trophic levels in response to multiple stressors. Yet, marine species from different trophic levels with dissimilar characteristics and evolutionary history are likely to respond differently to climatic stressors.Our study represents the first meta-analysis of multiple stressor studies to target comparisons of mean effects and identification of interaction types among marine trophic levels. The meta-analysis revealed a number of key results: (1) Predators are the most tolerant level in response to individual and combined effects of ocean acidification and warming; (2) synergistic interactions (16%) are less common than additive (40%) and antagonistic (44%) interactions; (3) interaction types vary among trophic levels, with the proportion of synergistic interactions decreasing with increasing trophic level; (4) for interactive effects, calcifying and non-calcifying species show similar patterns across trophic levels; and (5) trophic levels respond to stressors differently along a latitudinal gradient. This study emphasizes the importance of considering stressor interactions and trophic levels in conservation actions. Contrary to many predictions, which has suggested synergistic effects predominate multiple stressors, this research demonstrates that the interaction effect between ocean acidification (OA) and ocean warming (OW) can sometimes mitigate or even reduce negative effects, with additive and antagonistic interactions dominating. Our study provides new knowledge for understanding how multiple stressors may interactively affect marine trophic levels and highlighting the need for further research and a deeper understanding of multiple stressors in conservation efforts.
Effects of yoga on impulsivity in patients with and without mental disorders: a systematic review and meta-analysis of randomized clinical trials
Authors: Machado Yuri de Castro , Oliveira Mariana , Lima Jogiely Larissa Ferreira , Barghav Hermant , Varambally Shivarama , de Miranda Débora Marques , Romano-Silva Marco Aurélio 
Published: September 2023

Abstract
Background
Yoga can be used as a complementary intervention to conventional treatments, whether pharmacological or non-pharmacological. Sustained practice of yoga can generate a series of benefits for individuals' quality of life and improve their physical fitness.
Objective
To investigate the potential effects of yoga as an adjunct intervention in conditions involving impulse control issues, such as attention deficit hyperactivity disorder (ADHD), borderline personality disorder, bipolar affective disorder, and substance use disorders.
Methods
We performed a systematic review and meta-analysis of placebo-controlled, randomized trials of yoga in patients with impulsivity. PubMed, Web of Science, and Science Direct databases were searched for trials published up to January, 2023. Data were extracted from published reports and quality assessment was performed per Cochrane recommendations. The effect size was determined using unbiased Hedges' g with 95% confidence intervals (CIs) to assess different measurements of impulsivity.
Results
Out of 277 database results, 6 RCT were included in systematic review and 4, in meta-analysis. To assess the level of attention and impulsiveness, the following scales were analyzed: Barratt Impulsiveness, UPPS-P Impulsive Behavior scale, Conners’ Continuous Performance Test IIª and Conners’ Parent Rating Scale–Revised: Long. There was no significant effect in effect sizes from four studies on the effects of yoga on impulsivity didn’t show a significant effect (g = -0.06; 95% CI, -0.37–0.24; p = 0.69).
Conclusions
Yoga didn’t have a significant improvement in impulsivity when compared to placebo. There are many tools to assess impulsivity, but they mean different concepts and domains consisting in a weakness on comparison of yoga effects.
PROSPERO registration: CRD42023389088
Metabolic remodeling by the PD-L1 inhibitor BMS-202 significantly inhibits cell malignancy in human glioblastoma
Authors: Ji Tianhai , Yang Xueou , Wang Wenjun 
Published: September 2023

Abstract
Recent crystallographic studies have shown that BMS-202, a small molecule compound with a methoxy-1-pyridine chemical structure, interacts well with PD-L1 dimerization. However, its roles and mechanisms of BMS-202 in glioma have not yet been reported. The aims of this work were to study BMS-202 antitumor activity and its underlying mechanisms in glioma. In the study, the multi-omics and bioinformatics tools, a series of experiments, including CCK-8, flow cytometry, co-immunoprecipitation, siRNA transfection, PCR, western blot, and cell migration and invasion, were performed to investigate the underlying mechanisms of BMS-202 in glioma. BMS-202 was shown to inhibit glioma cell proliferation and invasion. Mechanistically, BMS-202 downregulated PD-L1 expression on the GBM cell surface and regulated PD-L1/AKT/BCAT1 signaling pathways in an mTOR-independent manner. BMS-202 is expected to delay metabolism and extend life span. Thus, our data identify that BMS-202 potentially acts as an effective candidate for patients diagnosed with GBM.
Effect of Cu and Fe PM components on mitochondrial dynamics in human colon CaCo-2 cells
Authors: Cervellati Franco , Benedusi Mascia , Casoni Alice , Trinchera Giulia , Vallese Andrea , Ferrara Francesca , Pietrogrande Maria Chiara , Valacchi Giuseppe 
Published: September 2023

Abstract
Atmospheric particulate matter (PM) is one of the most dangerous air pollutants of anthropogenic origin; it consists of a heterogeneous mixture of inorganic and organic components, including transition metals and polycyclic aromatic hydrocarbons. Although previous studies have been focused on effect of exposure to highly concentrated PM on respiratory and cardiovascular systems, emerging evidence supports a significant impact of air pollution on gastrointestinal (GI) tract connecting the exposure to outdoor stressors with conditions such as appendicitis, colorectal cancer, and inflammatory bowel disease. In general, it has been postulated that the main mechanism involved in PM toxicity consists in an OxInflammatory response and this has been also suggested for the GI tract. In the present study we want to analyze the effect of specific redox-active PM components, such as copper (Cu) and iron (Fe), in human intestinal cells by focusing on ultrastructural integrity, redox homeostasis and modulation of some markers related to mitochondria dynamics. According to our study in CaCo2 cells, the exposure to the Cu- and Fe-PM components and their combination induced ultrastructural alterations in the endoplasmic reticulum with an additive effect when the cells were exposed to both PM (Cu and Fe). In addition, the expression and the protein levels of genes involved in mitochondria dynamic were clearly affected by the exposure. In particular, Mitofusin 1 and OPA1 increased after the single exposure and the combination of both particles showed an additive effect. Opposite trend was noticed for PINK and PARKIN. In general, our data suggest that acute exposure to specific Cu and Fe metals, can induce morphological and molecular damage in CaCo-2 cells confirming the correlation noticed between living in polluted area and incidence of gastrointestinal tract conditions.
Dynamic Partial Order Reduction Based on Interference Solution
Authors: Yang Xu , Ye Chen 
Published: September 2023

Abstract
In verifying the process of concurrent programs, dynamic partial order reduction (DPOR) exhaustively explores all the interactions of the equivalent execution traces in the concurrent system through dynamic judgment. Existing DPOR algorithms strive for optimal exploration (i.e., exploring only one interleaving per equivalence class), leading to exponential redundancy in the interleaving, state-space explosion, and memory overflow. In this study, the Interference Solution (IntSo) algorithm is proposed to achieve linear memory consumption by exploring only one interleaving per equivalence class through the exchange of causally-happened-before (CHB) conflicting relationships. In the sequential consistency memory model, the IntSo algorithm realizes parallelism while curtailing time and memory consumption in data-independent scenarios, as well as maximum path lengths. Empirical validation using GenMC demonstrates that the IntSo algorithm compares favorably with state-of-the-art techniques in terms of time and memory, fulfills linear time complexity, and reduces memory consumption by more than 30%.
Association of Social Vulnerability Index and Masking Adherence Among Children Enrolled in COVID-19 Community Research Partnership Study
Authors: Dantuluri Keerti L. , Buahin Asare , Uschner Diane , DeWitt Michael , Rossman Whitney , Dunn Connell O. , Hetherington Timothy C. , Priem Jennifer , Castri Paola , Lagarde William H. , Gibbs Michael , Ahmed Amina 
Published: September 2023

Abstract
Background: Individuals with high social vulnerability index (SVI) have poorer outcomes with COVID-19. Masking reduces transmission of COVID-19 among children, but how SVI plays a role in masking behavior is unknown. We aimed to measure the association of SVI with masking adherence among children during the COVID-19 pandemic.
Methods: We conducted a multi-site, prospective syndromic surveillance study among children aged 2 – 17 years in the Southeastern United States by daily electronic surveys which solicited symptoms of COVID-19-like illness, infection with or exposure to SARS-CoV-2, masking habits, and any receipt of COVID-19 vaccines. Parents/guardians submitted surveys for their children; adolescents 13 years and older could opt to submit their own surveys. Multivariable and univariate linear models were used to measure the associations of different predictors such as SVI with masking adherence.
Results: 1,461 children from 6 states and 55 counties predominately from North and South Carolina were included in the analysis. Most children in the cohort were 5 – 11years old, non-Hispanic White, from urban counties, and with low-moderate SVI. Overall masking adherence decreased over time, and older children had higher masking adherence throughout the study period compared with younger children. Children who resided in urban counties had greater masking adherence throughout the study period than those who resided in suburban or rural counties. Masking adherence was higher among children with both low and medium SVI than those with high SVI.
Conclusions: Despite being at risk for more severe outcomes with COVID-19, children with high SVI had lower levels of masking adherence compared to those with low SVI. Our findings highlight opportunities for improved and targeted messaging in these vulnerable communities.
Endoscopic spinal cord untethering using a 1 cm skin incision technique in pediatrics: A technical case report
Authors: Ishisaka Eitaro , Tahara Shigeyuki , Tsukiyama Atsushi , Nozaki Toshiki , Hattori Yujiro , Morita Akio , Murai Yasuo 
Published: September 2023

Abstract
Background
Spinal cord untethering by sectioning the filum terminale is commonly performed in tethered cord syndrome patients with minor abnormalities such as filar lipoma, thickened filum terminale, and low conus medullaris.
Our endoscopic surgical technique, using the interlaminar approach, allows for sectioning the filum terminale through a very small skin incision. To our knowledge, this procedure has not been previously reported. This is the first case report involving a 1 cm skin incision.
Case presentation
A 9-month-old male patient was referred to our neurosurgical department due to a coccygeal dimple. MRI revealed a thickened fatty filum. After considering the treatment options for this patient, the parents agreed to spinal cord untethering. A midline 1 cm skin incision was made at the L4/5 vertebral level. Untethering by sectioning the filum terminale was performed by full endoscopic surgery using the interlaminar approach. The procedure was uneventful and there were no postoperative complications.
Conclusions
In terms of visibility and minimizing invasiveness, our surgical technique of using the interlaminar approach with endoscopy allows for untethering by sectioning the filum terminale through a very small skin incision.