bluuebunny/crossref_metadata_2025_split huggingface dataset:
The experience in dealing with abnormal signal intensities in Children with Neurofibromatosis type 1
Authors: Han Yong , Wang Hangzhou , Huang Yunlun 
Published: August 2023

Abstract
Background: We sought to define the radiologic features which could differentiate non-neoplastic from neoplastic T2 hyperintensities (T2Hs) identiﬁed on MRI in children with neurofibromatosis type 1(NF1) and identify lesions that most likely to require oncologic surveillance.
Methods: We conducted a single-center retrospective review of all available brain MRIs from 49 children with NF1 and 50 healthy pediatric controls. All T2Hs identified on MRI were characterized based on location, presence of T1 hypointensity, mass effect, contrast enhancement, edma, cystic lesion, or lesion related symptoms. Subsequently, all T2Hs were classified using newly established criteria as either unidentified bright objects (UBOs) or probable tumors. Lesion classification was pathologically confirmed in 6 NF1 cases.
Results: T2Hs were present in 35 (71.4%) individuals of the NF1 cohort. UBOs constituted the majority of T2Hs and were most frequently located in basal ganglia, cerebellar hemispheres, and brainstem. T2Hs of 7 NF1 patients were classified as probable tumors, and 6 children with probable tumors received surgical treatment. Five lesions of the six surgical children proved to be glioma.
Conclusion: T2Hs has been found to be a highly sensitive and specific marker for the diagnosis of NF1. With the application of standardized radiologic criteria, a high prevalence of probable brain tumors will be identiﬁed in this at-risk population of children, of which nearly 70% require treatment, emphasizing the need for appropriate oncologic surveillance for NF1 patients with probable brain tumors.
Preparation of Amino Cellulose aerogel and its formaldehyde adsorption Properties
Authors: Liu zhongzheng , Jiang Guiquan , Li Yaning , Chi Chuanxi , Zhang Yang , Song Jianxi 
Published: August 2023

Abstract
The aerogel prepared from cellulose has the advantages of low density, high porosity, large specific surface area, good gas diffusion and adsorption space, which is a good gas adsorption material. In this study, microcrystalline cellulose was used as raw material, LiBr ionic liquid was used as solvent system, cellulose aerogel was modified by polyethyleneimine, which retained three-dimensional network structure and had excellent formaldehyde adsorption capacity. The adsorption capacity of Polyethyleneimine-modified Cellulose Aerogel(PCC) to formaldehyde can reach 12.3 mg/g at 25℃. It was found that the adsorption capacity of PCC aerogel for formaldehyde changed with the increase of nitrogen content. The adsorption of formaldehyde was mainly through the chemical adsorption of Schiff base reaction between NH2 and formaldehyde molecules in Polyethylenimine(PEI).
Sustainable Innovation for Garri Roasting: Examining the Potential of Parabolic Dish Solar Collectors and Thermal Energy Storage
Authors: Sobarasua O. G. , Onuche I. F. , Kuye A. O. , Diemuodeke E. O. , Muritala I. K. , Saturday E. G. 
Published: August 2023

Abstract
Gari roasting is an energy-intensive process that is commonly used to produce gari, a popular food in Nigeria made from cassava. Traditional gari roasting methods use firewood as an energy source, which is not only inefficient but also harmful to the health of those who inhale the smoke (who are mainly women and children in rural communities) and contributes to deforestation. This work aims to investigate the use of a developed garri roasting system driven by a concentrated parabolic solar collector. Important garri roasting food chain data was obtained from a work-study at a rural community: temperatures, moisture content. The garri roasting temperatures range between 85oC - 100oC. The parabolic dish solar collector was designed and fabricated to achieve temperatures between 1180C-154oC at optimum tilt angles at Port Harcourt (4°54' 22.86''N, 6°55' 27.52''E) climatic conditions. The focal length ranges between 50mm-80mm, and the fluctuation of the focal point position was considered for the thermal storage system design. Gravel was used as a thermal storage and heat transfer enhanced through small internally placed mild steel rods. A theoretical estimate for total fry time per batch was 8.5 minutes. The garri roasting experiment was conducted for three days with favorable weather conditions, with an achieved maximum temperature of 101oC. This innovation is an important step in addressing the health risks associated with traditional gari roasting methods, and in moving towards zero-emission and zero-poverty innovative systems.
Long-term evolution and driving mechanisms of the Baiyangdian wetland based on land cover frequency characteristics
Authors: Guo Chao-fan , Zhao Dong-liang , Wu Dong-li 
Published: August 2023

Abstract
For sustainable conservation and management of wetlands, it is essential to quantify evolution progress and driving mechanisms of wetlands. This study examined Baiyangdian Wetland as a research area, and Landsat images and statistical yearbooks were used as data sources. Land cover type-dominant zones were innovatively defined based on spatial characteristics of land cover frequency. A transfer matrix and random forest were used to analyze the evolution process and driving mechanism of typical wetlands in the North China Plain. The conclusions were: (1) There was significant spatial differentiation of land cover frequency characteristics in the Baiyangdian wetland, with the cultivated land-dominated zone, the aquatic vegetation-dominated zone, and the water body-dominated zone being the three core zones of wetland ecosystem evolution. (2) There was a frequent and significant change in the land cover of the Baiyangdian wetland due to extreme climatic conditions. Between 1987 and 2017, the built-up land area and the water body area increased by 4.62 km2 and 16.17 km2, respectively, while the aquatic vegetation area decreased by 20.70 km2. (3) The driving mechanisms of ecosystem evolution in the Baiyangdian wetland were influenced significantly by space, time, and land use types. Specifically, on a spatial scale, social factors were the predominant factors contributing to changes in water body and cultivated land in the cultivated land-dominant zone and the aquatic vegetation-dominant zone. In contrast, natural factors were the dominant factors leading to changes in aquatic vegetation. There was a greater contribution from social factors to the driving mechanism of changes in the water body, aquatic vegetation, and cultivated land in the water body-dominant zone than from natural factors. On a temporal scale, it was primarily influenced by natural factors in the early stages and socio-economic factors in the late stages, with the year 2002 serving as a turning point. The scale of land use types can be seen primarily in the differences among the agricultural lands, aquatic vegetation, and water body. In particular, the Secondary Industry Out Value (SIOV) was highly sensitive to changes in the cultivated area. In addition, cumulative evaporation (EC) has been identified as the main factor responsible for the evolution of aquatic vegetation, which is closely related to the changes in water body.
Investigating Hydropericardium Syndrome with Different Histopathological Techniques in Broiler Chickens
Authors: Alavizadeh Hossein , Moosavi Zahra , Tabar Gholamreza Hashemi 
Published: August 2023

Abstract
Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, and dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium, and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages, and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia, and necrotizing enteritis were some of the findings reported in previous research. However, a low macrophage infiltration rate and brain lesions were novel discoveries in hematoxylin and eosin (H&E) examination. Therefore, Feulgen reaction and CD68 immunohistochemical staining were used for a comprehensive investigation, and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggests that HPS may cause brain damage, and the susceptibility of the Arian breed to the adenovirus needs further investigation.
Granulocyte colony-stimulating factor and decitabine-containing conditioning improves survival in myelodysplastic syndrome patients with iron overload undergoing allogeneic hematopoietic stem cell transplantation
Authors: Zhao Wenshu , Pan Danqi , Zeng Xiangzong , Xuan Li , Fan Zhiping , Huang Fen , Xu Na , Sun Jing , Liu Qifa , Dai Min 
Published: August 2023

Abstract

Background:Iron overload is considered as an unfavorable prognosis in myelodysplastic syndrome (MDS) even in those undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Although iron chelation therapy has improved the prognosis of these patients to some extent, the effect has not yet been satisfactory. This study aimed to investigate whether granulocyte colony-stimulating factor and decitabine (G-DAC)-containing conditioning improve the prognosis of iron-overloaded MDS patients undergoing allo-HSCT.Methods:One hundred and ninety-seven patients were enrolled in this retrospective study. Based on the level of serum ferritin (SF) and conditioning regimen, all patients enrolled were divided into 4 groups: SF﹤1000µg/L with G-DAC conditioning (cohort 1), SF﹤1000µg/L with non-G-DAC conditioning (cohort 2), SF ≥ 1000µg/L with G-DAC conditioning (cohort 3) and SF ≥ 1000µg/L with non-G-DAC conditioning (cohort 4). The clinical features and prognosis between cohorts were analyzed.Results:The 2-year overall survival (OS) was 77.0%, 72.1%, 73.8% and 38.7% (P= 0.001), and disease-free survival (DFS) was 75.7%, 62.8%, 71.7% and 35.5% (P= 0.001), and the cumulative incidence of non-relapse mortality (NRM) was 17.3%, 25.0%, 15.2% and 53.1% (P= 0.001), and the incidence of relapse was8%, 13.6%, 13.1% and 12.5% (P= 0.592), respectively, in the four groups. To be specific, cohort4 had worse OS and DFS and higher NRM than the other three groups (allP﹤0.05). Multivariate analysis revealed that SF ≥ 1000µg/L was a risk factor for OS, DFS and NRM (P= 0.022,P= 0.025,P= 0.036), while G-DAC-containin g conditioning was a protective factor (P= 0.009,P= 0.003,P= 0.004). Intriguingly, when cohort 1 to cohort4 were included in the multivariate analysis, only cohort4 was a risk factor for OS, DFS and NRM (allP﹤0.05), but cohort3, namely iron-overloaded patients who received G-DAC-containing conditioning had no difference in prognosis compared with patients with SF﹤1000µg/L.Conclusions:The poor prognosis of patients with iron overload could be overcome byG-DAC-containing conditioning partly.
Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis
Authors: zhouqiao Qiao , Liu Jian , Hu Yuedi , Qi Yajun 
Published: August 2023

Abstract
Objective
We intended to explore the anti-inflammatory and chondroprotective effects of Xinfeng capsule (XFC) in osteoarthritis (OA), perhaps through the regulation of hsa_circ_0032131 and miR-502-5p/TRAF2 axis.
Materials and methods
In total, 30 patients with OA and healthy subjects were recruited. To detect markers of cartilage metabolism and inflammation, peripheral blood mononuclear cells (PBMCs) were taken out. Subsequently, network pharmacology was employed to forecast OA-related targets and pathways for XFC therapy. To investigate the function of upregulated hsa_circ_0032131 in model cells.A series of functional experiments revealed the relationship between hsa_circ_0032131 and miR-502-5p/TRAF2 axis. To further determine whether XFC potentially treats OA through the interaction between circ_0032131 and miR-502-5p/TRAF2 axis. CKK-8 assay and flow cytometry were performed to detect cell proliferation and apoptotic processes in XFC-treated cells. Some conventional experimental methods were used to detect the expression levels of inflammatory factors, extracellular matrix and others. In addition, rescue experiments verified that XFC blocked the effects of hsa_circ_0032131 overexpression on extracellular matrix, inflammation and cell viability.
Results
Clinical observations indicated that the expression of hsa_circ_0032131 in PBMCs of OA patients was significantly elevated, and there was a correlation with clinical immuno-inflammatory factors and inflammatory indicators. Network pharmacology verified that the chief active ingredients of XFC exerted their roles mainly in the regulation of inflammation (IL1A, IL1B, IL4), extracellular matrix metabolism (MMP13, COL2A1), and tumour necrosis factor (TNF, TRAF2). In vitro experiments revealed that knockdown of circ_0032131 inhibited apoptosis, inflammatory and ECM degradation in PBMCs-stimulated chondrocytes. Circ_0032131 was verified to be a sponge of miR-502-5p by targeting, and TRAF2 was a direct target of miR-502-5p. By regulating circ_0032131 and miR-502-5p/TRAF2 axis, XFC prevented PBMCs-stimulated chondrocytes from responding to inflammation and ECM degradation.
Conclusion
The XFC suppressed inflammatory response and extracellular matrix metabolism in OA by regulating circ_0032131 and miR-502-5p/TRAF2 axis.
SAS: Split Antibiotic Selection for identifying chaperones that improve protein solubility
Authors: McNutt Emily , Ke Na , Thurman Alexandre , Eaglesham James B. , Berkmen Mehmet 
Published: August 2023

Abstract
Background
Heterologous expression of active, native-folded protein in Escherichia coli is critical in both academic research and biotechnology settings. When expressing non-native recombinant proteins in E. coli, obtaining soluble and active protein can be challenging. Numerous techniques can be used to enhance a proteins solubility, and largely focus on either altering the expression strain, plasmid vector features, growth conditions, or the protein coding sequence itself. However, there is no one-size-fits-all approach for addressing issues with protein solubility, and it can be both time and labor intensive to find a solution. An alternative approach is to use the co-expression of chaperones to assist with increasing protein solubility. By designing a genetic system where protein solubility is linked to viability, the appropriate protein folding factor can be selected for any given protein of interest. To this end, we developed a Split Antibiotic Selection (SAS) whereby an insoluble protein is inserted in-frame within the coding sequence of the hygromycin B resistance protein, aminoglycoside 7″-phosphotransferase-Ia (APH(7″)), to generate a tripartite fusion. By creating this tripartite fusion with APH(7″), the solubility of the inserted protein can be assessed by measuring the level of hygromycin B resistance of the cells.
Results
We demonstrate the functionality of this system using a known protein and co-chaperone pair, the human mitochondrial Hsp70 ATPase domain (ATPase70) and its co-chaperone human escort protein (Hep). Insertion of the insoluble ATPase70 within APH(7ʹʹ) renders the tripartite fusion insoluble and results in sensitivity to hygromycin B. Antibiotic resistance can be rescued by expression of the co-chaperone Hep which assists in the folding of the APH(7ʹʹ)-ATPase70-APH(7ʹʹ) tripartite fusion and find that cellular hygromycin B resistance correlates with the total soluble fusion protein. Finally, using a diverse chaperone library, we find that SAS can be used in a pooled genetic selection to identify chaperones capable of improving client protein solubility.
Conclusions
The tripartite APH(7ʹʹ) fusion links the in vivo solubility of the inserted protein of interest to hygromycin B resistance. This construct can be used in conjunction with a chaperone library to select for chaperones that increase the solubility of the inserted protein. This selection system can be applied to a variety of client proteins and eliminates the need to individually test chaperone-protein pairs to identify those that increase solubility.
MiR-1a-3p inhibits apoptosis in fluoride-exposed LS8 cells by targeting Map3k1
Authors: Chen Ting , Gu Yu , Bai Guo-Hui , Liu Xia , Chen Bin , Fan Qin , Liu Jian-Guo , Tian Yuan 
Published: August 2023

Abstract
Dental fluorosis is a chemical disease that is common.At present, it is not clear how fluorosis happens at the molecula level.We have used miRNA-seq to look at the differences between miRNAs in the cell line of ameloblasts LS8 cells that had been treated with 3.2 mmol/L NaF. and did GO and KEGG pathway enrichment analysis .We found that the levels of miR-1a-3p were significantly lower in mouse LS8 cells treated with 3.2 mmol/L NaF, and the genes that miR-1a-3p targets were significantly enriched in the MAPK pathway. The LS8 cells were divided into four groups: the control, NaF, NaF + miR-1a-3p mimics and NaF + miR-1a-3p mimics NC groups. The morphology of the cells were observed by an inverted microscope.We detected the proliferation activity of LS8 cells by Cell Counting Kit-8 (CCK-8). Using the real-time quantitative polymerase chain reaction (RT-qPCR), we detected the transcription levels of miR-1a-3p and Map3k1. The expressions of Bax, Bcl-2, Map3k1, p38MAPK, ERK1/2, p-p38MAPK and p-ERK1/2 were measured by Western Blot. After doing a bioinformatics analysis, we used a luciferase reporter assay (LRA) to validate the target of miR-1a-3p.Our results indicated that miR-1a-3p could inhibit the apoptosis while increasing proliferation in fluoride-exposed LS8 cells.In general, miR-1a-3p may directly inhibit Map3k1, reduce MAPK signal pathway activation, and promote phosphorylation.Thus, Our findings revealed that miR-1a-3p's interaction with its target gene Map3k1 and MAPK signal pathway might decrease LS8 cells' apoptosis treated with 3.2 mmol/L NaF.
LncRNA USP2-AS1 facilitates colorectal cancer development via upregulating PHLDA2 through recruiting IGF2BP2 and absorbing miR-134-5p
Authors: Zhu Jing , Liu Zichun , Chang Lisha , Gu Qiou , Lin Shuhui , Luo Qian , Qian Mengseng , Ding Jie , Wei Qiong , Pu Juan , Wang Keming 
Published: August 2023

Abstract
Background
Colorectal cancer (CRC) is one of the most common malignant tumors and a challenging public health issue worldwide, seriously threatening human health. It is essential to explore further the molecular mechanisms involved in the occurrence and development of CRC and identify new biomarkers and therapeutic targets for CRC. Researchers have revealed that long non-coding RNAs (lncRNAs) are involved in multiple cancers development, including CRC. USP2-AS1 is a newly discovered lncRNA whose function in CRC has yet to be fully elucidated, prompting us to study further the roles and potential mechanisms of USP2-AS1 in CRC.
Methods
The expression of USP2-AS1 in CRC tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The biological function of USP2-AS1 in CRC was studied through Cell Counting Kit-8 (CCK-8) assays, colony formation assays, 5-Ethynyl‐2ʹ‐deoxyuridine (EdU) assays, migration assays, apoptosis assays, and animal experiments. The interaction between USP2-AS1, PHLDA2, IGF2BP2, and miR-134-5p was revealed through bioinformatics analysis, RNA sequencing, RNA stability assays, RNA Immunoprecipitation (RIP) assays, and dual-luciferase reporter assays.
Results
We discovered that USP2-AS1 was overexpressed in CRC tissues and cell lines, and USP2-AS1 overexpression was relevant to poor prognosis in CRC patients. Functional experiments clarified that USP2-AS1 facilitated CRC cell growth and metastasis and reduced apoptosis. Additionally, animal experiments demonstrated that USP2-AS1 could promote tumor growth in vivo. Mechanistically, on the one hand, we verified that USP2-AS1 could bind to IGF2BP2 and thus stabilize PHLDA2 mRNA. On the other hand, USP2-AS1 could absorb miR-134-5p to regulate PHLDA2 expression.
Conclusions
USP2-AS1 could upregulate PHLDA2 expression by recruiting IGF2BP2 and competitively binding miR-134-5p, thus facilitating CRC malignant progression. Our
The complete mitochondrial genome of Octopus vulgaris
Authors: Fee Gareth N. , Roura Alvaro , Emami-Khoyi Arsalan , Teske Peter R. 
Published: August 2023

Abstract
Background
The Octopus vulgaris species complex consists of numerous morphologically similar but genetically distinct species. The current publicly available mitogenome of this species has been generated from a specimen collected from Tsukiji Fish Market, Tokyo, Japan. Octopus from the northwestern Pacific Ocean are now considered to be a separate species, Octopus sinensis. For this reason, we hypothesised that the current record of O. vulgaris was sequenced from a specimen of O. sinensis. Here, we sequenced the first complete mitogenome of a specimen of Octopus vulgaris sensu stricto that was collected from the species’ confirmed distribution areas in northeastern Atlantic.
Methods and results
The complete mitogenome was assembled de novo and annotated using 250 bp paired-end sequences. A single circular contig 15655 bp in length with a mean read coverage of 1089 reads was reconstructed. The annotation pipeline identified 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNA) and two ribosomal RNAs (rRNA). A Bayesian phylogenetic reconstruction recovered the assembled mitogenome as the sister taxon of a monophyletic group comprising O. sinensis and the previously published mitogenome of “O. vulgaris” from Japan. This confirms that the latter was a Japanese specimen of O. sinensis.
Conclusion
The mitogenome sequenced here is the first to be published for Octopus vulgaris sensu stricto. It represents an important first step in genetics-informed research on the evolution, conservation, and management of this commercially important species.
Importance of Swamp Forest Fragments for the Habitat and Conservation of Vertebrates
Authors: Arriagada Aldo , Fuentes Norka 
Published: August 2023

Abstract
The Temu-Pitra swamp forests in Chile support great biodiversity, however, they are highly threatened by anthropic disturbances that increase the dragging of sediments. Between 2020 and 2021 we recorded vertebrates in a swamp forest fragment in southern Chile, and identified 41 species of birds and eight mammals. Nine species of birds and two species of mammals are strictly aquatic and have greater dependence for their survival on the remnant of swamp forest studied. This swamp forest fragment provides critical habitat for strictly aquatic birds and mammals, but also for terrestrial species (birds and carnivores) with extensive home ranges. The swamp forest is inserted in a sub-basin forest matrix that threatens its conservation, through the dragging and deposition of sediment in the bodies of water. Although the swamp forest fragment studied presented a less diverse vertebrate community compared to other local wetlands, we suggest that it plays an important role in the conservation of these vertebrates in southern Chile, where the development of forestry activity continues to produce fragmentation and loss of habitat, in addition to the deterioration of water resources.