bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extraction of flavonoids from the rind of Passiflora edulis f. Flavicarpa by pressurized solvent extraction and aqueous two-phase systems
Authors: Pinto Paloma Lira , Siqueira Ana Cristina de Abreu , de Sant’Ana Hosiberto Batista , de Brito Edy Sousa , Silva Ivanildo 
Published: June 2023

Abstract

As the rind of passion fruit is rich in flavonoids, it can be used as an alternative to obtain these compounds. This study extracted flavonoids from passion fruit rinds by pressurized solvent extraction. The obtained extract was submitted to aqueous two-phase extraction formed by ethanol and salts for the recovery of flavonoids. Passion fruit rind (PFR) was submitted to pressurized solvent extraction (PSE) using ethanol/water solution at 60:40 (v/v) as a solvent for the extraction of bioactive compounds, showing a polyphenol content of 5.48 gGAE∙kg− 1 PFR. Then, aqueous two-phase systems (ATPS) showed that flavonoids and sugars partitioned into opposite phases. The recovery of flavonoids in the top phase was above 98% w/w, and the recovery of sugars in the bottom phase was above 97% w/w. LC‒MS was used to evaluate the flavonoid profile in the top phase of ATPS, and the major flavonoids detected were lucenin-2, vicenin, isoorientin, apigenin, schaftoside and vitexin. According to the flavonoid profile by LC‒MS and the values of recovery of flavonoids, ATPS proved to be an effective technique for recovery of flavonoids from PSE extraction.
Comparative Transcriptome Analysis Reveals Candidate Genes For Cold Stress Response and Early Flowering in Pineapple
Authors: Yow Ashley G. , Laosuntisuk Kanjana , Young Roberto , Doherty Colleen J. , Gillitt Nicholas , Perkins-Veazie Penelope , Xiang Qiu-Yun Jenny , Iorizzo Massimo 
Published: June 2023

Abstract

Pineapple originates from tropical regions in South America and is therefore significantly impacted by cold stress. Periodic cold events in the equatorial regions where pineapple is grown may induce early flowering, also known as precocious flowering, resulting in monetary losses due to small fruit size and the need to make multiple passes for harvesting a single field. Currently, pineapple is one of the most important tropical fruits in the world in terms of consumption, and production losses caused by weather can have major impacts on worldwide exportation potential and economics. To further our understanding of and identify mechanisms for low-temperature tolerance in pineapple, and to identify the relationship between low-temperature stress and flowering time, we report here a transcriptomic analysis of two pineapple genotypes in response to low-temperature stress. Using meristem tissue collected from precocious flowering-susceptible MD2 and precocious flowering-tolerant Dole-17, we performed pairwise comparisons and weighted gene co-expression network analysis (WGCNA) to identify cold stress, genotype, and floral organ development-specific modules. Dole-17 had a greater increase in expression of genes that confer cold tolerance. The results suggested that low temperature stress in Dole-17 plants induces transcriptional changes to adapt and maintain homeostasis. Comparative transcriptomic analysis revealed differences in cuticular wax biosynthesis, carbohydrate accumulation, and vernalization-related gene expression between genotypes. Cold stress induced changes in ethylene and abscisic acid-mediated pathways differentially between genotypes, suggesting that MD2 may be more susceptible to hormone-mediated early flowering. The differentially expressed genes and module hub genes identified in this study are potential candidates for engineering cold tolerance in pineapple to develop new varieties capable of maintaining normal reproduction cycles under cold stress. In addition, a total of 461 core genes involved in development of reproductive tissues in pineapple were also identified in this study. This research provides an important genomic resource for understanding molecular networks underlying cold stress response and how cold stress affects flowering time in pineapple.
Identification and validation of disulfidptosis-related subtypes and the prognostic model in hepatocellular carcinoma
Authors: Huang Qiming , Li Tiewen , Liu Sisi , Chen Difei , Du Yemei , Jie Lei , Ma Yang , Hong Ru-Tao 
Published: June 2023

Abstract
Background
Disulfidptosis is a newly discovered form of cell death. The latest reports have revealed potential mechanisms for disulfide-mediated cell damage, including actin cytoskeleton proteins and cellular scaffold proteins, which are susceptible to disulfide stress. Accumulation of disulfides in cells results in the breakdown of the actin network and cell death. However, the prognostic role and regulatory mechanism of disulfidptosis-related genes in hepatocellular carcinoma remain unclear.
Methods
The differential expression, copy number variation, and prognostic relevance of 10 genes associated with disulfidptosis were analyzed. Based on the expression levels of the disulfidptosis-related genes, unsupervised clustering analysis was performed to classify the samples into three subtypes, and their gene expression, biological functions, and immune cell infiltration were analyzed. Subsequently, the common differentially expressed genes were screened and a gene prognostic model was established. Samples with high-risk scores showed higher immune cell infiltration and expression of immune checkpoint genes.
Results
Firstly, we found that four genes (LRPPRC, NCKAP1, RPN1, SLC7A11) showed significant prognostic ability for overall survival in HCC patients. Subsequently, using consensus clustering analysis, the samples were divided into three clusters(A, B and C cluster) based on the expression levels of the four prognostic disulfidoptosis genes, where the prognostic of B cluster was worse, and the cell cycle pathway activation was significantly enriched. Furthermore, the immune cell infiltration abundance was calculated, significant differences in TME were observed among the three subtypes. Additionally, among the common differentially expressed genes among the three subtypes, lasso regression was used to screen six survival-related genes, and a prognostic model was established. Finally, our results suggest that the risk score may serve as a useful tool for predicting sensitivity to immunotherapy and drug treatment in HCC patients.
Conclusion
These findings highlight the significant clinical implications of the sulfide death pathway and provide new insights into guiding personalized immune therapy strategies for patients with hepatocellular carcinoma.
Coagulase-negative Staphylococci from Bovine Milk: Virulent gene detection, and Antibiogram Profiles
Authors: Getahun Yared Abate , Abey Solomon Lulie , Beyene Achenef Melaku , Belete Mequanint Addisu , Tessema Tesfaye Sisay 
Published: June 2023

Abstract

Background Coagulase-negativeStaphylococcusspecies are an emerging cause of intramammary infection, posing a significant economic and public health threat. The cross-sectional study was conducted to isolate and assess virulence factors and antibiogram profiles of coagulase-negativeStaphylococcusspecies in 290 milk samples collected from February to August 2022. Species isolation and identification were performed by plate culturing and biochemical tests and single-plex PCR was used for the detection of virulent genes. The antimicrobial susceptibility profile of each coagulase-negativeStaphylococcusspecies isolate was determined by the Kirby-Bauer disc diffusion test. Results Coagulase-negativeStaphylococcusspecies were isolated in 28.6%, (83/290) of the samples. Of these, theS. epidermidis,S. sciuri,S. warneri,S. haemolyticus,S. simulans,S. chromogens,S. cohnii, andS. captisspecies were isolated at the rates of 11, 5.2, 3.4, 3.1, 3.1, 1, 1, and 0.7% respectively. Coagulase-negative Staphylococcusspecies carried 26.5, 22.1, 21.7, 9.6, 9.6 and 8.4% of theica D,Pvl,Mec A,hlb,Sec, andhlavirulent genes respectively. All the isolates showed a high percentage (100%) of resistance to Amoxicillin, Ampicillin, and Cefotetan and 37.5% of resistance to Oxacillin. The majority (54.2%) of coagulase-negative isolates also showed multidrug resistance. Conclusion The present study revealed that coagulase-negativeStaphylococcusspecies isolates were found carriage of one or more virulent and enterotoxin genes responsible for intramammary and food poisoning infections. The majority of the isolates (54.2%) were also found multidrug resistant. Thus, urgent disease control and prevention measures are warranted to reduce the deleterious impact of coagulase-negative species. This study is the first to document the detection of virulent and food poisoning genes in coagulase-negativeStaphylococcusspecies from milk in Ethiopia.
Gender Differences in Severity and Parental Estimation of Adolescent’s Pandemic-Related Stress in the United States
Authors: Simkus Andrew , Holtz Kristen , Twombly Eric , Wanty Nicole 
Published: June 2023

Abstract
Background: Research has consistently shown that female adolescents have experienced worse pandemic-related stress compared to males. A parent’s ability to accurately track their child’s stress levels likely increases the likelihood a problem is acknowledged and addressed as it arises. Therefore, we assessed how parents’ estimation of their adolescent children’s self-reported pandemic-related stress related to the child’s gender.
Methods: We performed cross-sectional secondary analysis using the nationally representative Population Assessment of Tobacco and Health study datasets from Wave 5 (2018-2019) and Wave 5.5 (July 2020-December 2020) among respondents aged 12-17. We conducted four logistic regression models to explore the relationship between child gender and parental underestimation of their child’s pandemic-related stress. We controlled for sociodemographic factors and personal characteristics associated with pandemic-related stress including, whether the adolescent had been diagnosed with COVID-19, the extent social distancing measures were practiced, school performance, previous year anxiety, depression, and overall mental health ratings, sleep trouble, TV screen time, and past year substance use.
Results: Even when controlling for these factors, female child gender was significantly and positively associated with parental underestimation of their child’s pandemic-related stress (Underestimated stress: OR = 1.25 95 % CI = [1.07-1.46]).
Conclusions: Informing parents that female adolescents were significantly more likely to have their levels of pandemic-related stress underestimated at home may encourage parents to take extra effort when checking in on their daughters’ mental health needs, which in turn may lead to more female adolescents receiving the familial and professional support they require.
Listening to music while running does not impair lateral balance or increase ground impact forces in non-fatigued novice treadmill runners
Authors: Cunningham Neil James , Zordan Rachel 
Published: June 2023

Abstract
Novice runners have neither acquired the skill of running nor embedded an efficient biomechanical technique. Removing auditory cues for feedback by listening to music was theorised to impair the ability to register or respond to heavy or uncoordinated step impacts, potentially exposing the novice runner to increased forces transmitted through to the lower limb. The primary objective was to determine whether removing auditory feedback cues affected biomechanical running gait stability in novice runners as measured by step width and step width variability. Secondary objectives included analysis of the effect of listening to music on ground reaction force (GRF) and running symmetry. In a sample of novice runners (n = 23), repeated measure analysis found no significant differences across all outcome variables comparing music and non-music conditions. Novice runners who listen to music were not exposed to higher GRF or obvious balance impairment while running on treadmills in a non-fatigued state.
Integration of small RNAs, transcriptome and degradome sequencing reveals the mechanism of high photosynthetic efficiency in Medicago ruthenica (L.)
Authors: Shi Fengling , Gao Cuiping , Zhang Yutong , Yan Wei , Qiao Yu , Gao Xia , Tang Fang , Yi Fengyan 
Published: June 2023

Abstract
Background
Medicago ruthenica, a superior leguminous forage, is strongly drought-resistant and has wide adaptability. It is closely related to Medicago sativa (a commonly cultivated forage). Characterized by its high tolerance to environmental stress, M. ruthenica is a valuable genetic resource. However, its low yield limits its large-scale promotion and utilization. Leaf morphology, an important agronomic trait, is closely related to forage yield and photosynthetic efficiency.
Results
In this study, leaf width and leaf area were found significantly positively correlated with forage yield in M. ruthenica. Specifically, the cultivar M. ruthenica ‘Mengnong No.1’ (MN No.1) with a larger leaf area had higher photosynthetic efficiency. Furthermore, the key miRNA-target gene networks associated with high photosynthetic efficiency of M. ruthenica were identified using small RNAs and transcriptome sequencing. Totally, 63 differentially expressed miRNAs (DEMs) were identified, including some known miRNAs (miR408, miR171, and miR398, etc.). These known miRNAs corresponded to 55 target genes; 6 miRNA-target gene pairs had negatively correlated expression patterns. Degradome sequencing analysis further confirmed the authenticity of miR408-targeted cleavage of LAC5; namely, the miR408-LAC5 module may mediate the mechanism of high photosynthetic efficiency formation in M. ruthenica.
Conclusions
This study for the first time proposed that leaf morphology as a key trait affecting the high-yielding plant type of M. ruthenica. The key gene modules screened in this study may provide new insights into the elucidation of photosynthetic efficiency mechanism and the bio-design breeding of legume forages in the future.
Predictors of Efficacy of Anamorelin in Non-small-cell Lung Cancer Patientswith Cachexia: A Retrospective Study
Authors: Ishioka Yoshiko , Tanaka Hisashi , Makiguchi Tomonori , Fujishima Syunsuke , Nunomura Yasuhito , Sakamoto Hiroaki , Shiratori Toshihiro , Taima Kageaki , Tasaka Sadatomo 
Published: June 2023

Abstract
PURPOSE: Anamorelin, a ghrelin receptor agonist, has recently been approved in Japan for the treatment of cachexia in patients with lung and gastrointestinal cancer. However, only a few studies have investigated whether anamorelin is actually useful in clinical settings. We therefore investigated its efficacy in terms of patient characteristics.
METHODS: We retrospectively evaluated 40 patients with non-small-cell lung cancer with cachexia who were prescribed anamorelin at our department between July 2021 and November 2022. A logistic regression analysis was used to analyze the association between patients’ background characteristics and early discontinuation (within 4 weeks).
RESULTS: The early discontinuation group included 11 patients (27.5%). An Eastern Cooperative Oncology Group Performance Status (ECOG-PS) of ≥2 (odds ratio 7.85, 95% confidence interval 1.43-43.21, p=0.018) was associated with early discontinuation. Eighteenpatients (45.0%) were able to continue administration for 12 weeks, and the mean change in body weight was +2.31 kg, which was a significant change (p=0.027). The mean changesin lean body mass and soft lean mass were +1.97 kg (p=0.14) and +1.26 kg (p=0.15), respectively.
CONCLUSION: The study confirmed that anamorelin was unlikely to be useful in patients with a poor general condition (ECOG-PS ≥2).
The Added Value of Systematic Sampling in In-Bore Magnetic Resonance Imaging Guided Prostate Biopsy
Authors: Drori Tomer , Zilberman Dorit , Portnoy Orith , Dotan Zohar , Rosenzweig Barak , Lazarovich Alon 
Published: June 2023

Abstract
We sought to quantify the additive value of systematic biopsy (SB) using in-bore magnetic resonance (MR)-guided prostate biopsy (IBMRGpB) by retrospectively reviewing the records of 189 patients who undergone IBMRGpB. Endpoints included clinically significant and non-clinically significant cancer diagnosis. SB detected clinically significant disease in 67 (35.5%) patients. Five (2.65%) patients whose targeted biopsies found benign or non-clinically significant disease had clinically significant disease on SB. SB from the lobe contralateral to the lesion detected clinically significant disease in 15 (12%) patients. The size of the prostate was larger, and the percentage of lesions located in the peripheral zone of the prostate was higher in patients with SB-detected clinically significant disease. The location of the main lesion in the peripheral zone of the prostate was a predictor for clinically significant disease in multivariate analysis (OR = 8.26, p = 0.04), a finding supported by a subgroup analysis of biopsy-naïve patients (OR = 10.52, p = 0.034). The addition of SB during IBMRGpB increased the diagnosis of clinically significant as well as non-clinically significant prostate cancer. The location of the main lesion in the peripheral zone emerged as a positive predictive factor for clinically significant disease on SB. These findings may enhance patient-tailored management.
Flax for seed or fibre use? Flax capsules from ancient Egyptian sites (3rd millennium BC to 2nd century AD) compared with modern genebank flax accessions
Authors: Karg Sabine , Spinazzi-Lucchesi Chiara , Diederichsen Axel 
Published: June 2023

Abstract

In order to determine whether ancient Egyptians had already selected and cultivated very specialized flax types according to their purpose for textile or oil production we compared archaeobotanical flax finds that are kept at the Botanical Museum of the Free University in Berlin, Germany with diverse genebank accessions kept at the Plant Gene Resources of Canada (PGRC). The main result was that fibre flax was most probably already cultivated in Egypt during the the 3rd millennium BC, whereas in all later periods an intermediate flax and/or oil type was in use. The collaboration between archaeobotanists and genebank curators shows how knowledge about ancient usage of plants can be generated by interpreting observations made on ancient findings using characterization data obtained from diverse genebank material.
Modified pine needles as a formaldehyde scavenger for urea-formaldehyde resin in plywood production
Authors: Dukarska Dorota , Kawalerczyk Jakub , Kmieciak Jakub 
Published: June 2023

Abstract

The aim of presented work was to investigate whether it is possible to use ground pine needles as a formaldehyde-scavenging filler for urea-formaldehyde resin in the production of plywood. The scope of the research included determinations of both optimal amount of introduced filler and the effect of its modification (silanization, hydrothermal and alkaline treatments). Properties of adhesives such as viscosity, gel time and pH were investigated and their morphology was assessed using scanning electron microscopy. Manufactured plywood were tested in terms of wet shear strength, tendency to delamination and formaldehyde emission. It was found that the addition of pine needles lowers the pH and reduces gel time of the adhesive. Moreover, it was shown that despite a significant reduction in formaldehyde emission, the addition of non-modified needles causes a decrease in bonding quality of plywood. Based on the results, 10 parts by weight of needles per 100 parts by weight of resin can be considered as optimal loading. The use of ground needles modified hydrothermally and with silane allows to minimize the negative effect on the strength of glue lines and leads to further reduction of formaldehyde emission. Therefore, it can be concluded that pine needles powder has a strong potential for the application as formaldehyde-scavenging filler for urea-formaldehyde adhesive in plywood production.
Predictive factors associated with favourable neurological outcomes after extracorporeal cardiopulmonary resuscitation for out-of-hospital cardiac arrest: a retrospective multicentre cohort study
Authors: Tominaga Naoki , Takiguchi Toru , Seki Tomohisa , Hamaguchi Takuro , Nakata Jun , Yamamoto Takeshi , Tagami Takashi , Inoue Akihiko , Hifumi Toru , Sakamoto Tetsuya , Kuroda Yasuhiro , Yokobori Shoji 
Published: June 2023

Abstract

Background: Knowledge of the relative contributions of various factors to patient outcomes is crucial for optimal patient selection for extracorporeal cardiopulmonary resuscitation (ECPR) after out-of-hospital cardiac arrest (OHCA). However, evidence pertaining to their effects on neurological outcomes is limited. In this study, we investigated the predictive factors associated with favourable neurological outcomes after ECPR for OHCA.
Methods: We performed a secondary analysis of the SAVE-J II data, a retrospective multicentre registry study involving 36 participating institutions in Japan. Between 2013 and 2018, 2157 patients with OHCA who underwent ECPR were enrolled in SAVE-J II. Adult patients (aged ≥ 18 years) with OHCA, who underwent ECPR before admission to the intensive care unit (ICU), were included in our secondary analysis. Our primary outcome was a favourable neurological outcome at hospital discharge, defined as a Cerebral Performance Category (CPC) score of 1 or 2. We used a multivariate logistic regression model to examine the association between predictive factors measured at the incident scene or upon hospital arrival, and favourable neurological outcomes.
Results: A total of 1823 patients met study inclusion criteria. Multivariable analysis revealed that shockable rhythm at the scene of the OHCA (odds ratio (OR), 2.72; 95% confidence interval (CI), 1.43−5.38) and upon hospital arrival (OR, 2.56; 95% CI, 1.57−4.26), as well as unsustained return of spontaneous circulation (ROSC) (OR, 1.94; 95% CI, 1.02−3.61), gasping (OR, 4.67; 95% CI, 2.80−7.82), and pupillary reflex on arrival (OR, 3.06; 95% CI, 1.82−5.14) were significantly associated with favourable neurological outcomes.
Conclusions: This retrospective multicentre cohort study suggests that shockable rhythm, unsustained ROSC, gasping, and pupillary reflex were associated with particularly favourable neurological outcomes in patients with OHCA undergoing ECPR.
Thermodynamic equilibrium of Al 2 SiO 5 polymorphs in the triple point as a function of rock and fluid composition
Authors: Artimenko Margaret V. 
Published: June 2023

Abstract
For decades the geologists have discussed whether the Al2SiO5 polymorphs (andalusite, kyanite and sillimanite) can co-exists in thermodynamic equilibrium in a real rock at P–T conditions of the triple point or not. A triple-point rock sample was not so far identified reliably in nature. The thermodynamic-potentials minimization technique allows us to determine the bulk rock and fluid composition, other minerals in the assemblage, and other factors that could influence the stability of aluminosilicates. All three polymorphs were predicted to occur simultaneously in a mineral assemblage at P–T conditions of the triple point. Simple mineral assemblages consisting of plagioclase ± quartz ± paragonite ± graphite, biotite ± muscovite ± quartz ± magnetite ± graphite were studied in order to determine the conditions of the stable coexistence of the Al2SiO5 polymorphs. The study demonstrates how the hydrogen-, oxygen- and carbon-content in fluid can define the mineral assemblage and the coexistence of the Al2SiO5 polymorphs in it. The effect of such elements as Na, Fe, Mg, Mn, Ti and P was studied using bulk compositions of natural rock samples. A series of restrictions on the bulk composition of the host rock was identified.