bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arsenic disrupts extracellular vesicle-mediated signaling in regenerating myofibers
Authors: Clemens Zachary , Wang Kai , Ambrosio Fabrisia , Barchowsky Aaron 
Published: August 2023

Abstract
Chronic exposure to environmental arsenic is a public health crisis affecting hundreds of millions of individuals worldwide. Though arsenic is known to contribute to many pathologies and diseases, including cancers, cardiovascular and pulmonary diseases, and neurological impairment, the mechanisms for arsenic-promoted disease remain unresolved. This is especially true for arsenic impacts on skeletal muscle function and metabolism, despite the crucial role that skeletal muscle health plays in maintaining cardiovascular health, systemic homeostasis, and cognition. A barrier to researching this area is the challenge of interrogating muscle cell-specific effects in biologically relevant models. Ex vivo studies investigating mechanisms for muscle-specific responses to arsenic or other environmental contaminants primarily utilize traditional 2-dimensional culture models that cannot elucidate effects on muscle physiology or function. Therefore, we developed a contractile 3-dimensional muscle construct model—composed of primary mouse muscle progenitor cells differentiated in a hydrogel matrix—to study arsenic exposure impacts on skeletal muscle regeneration. Muscle constructs exposed to low-dose (50 nM) arsenic exhibited reduced strength and myofiber diameter following recovery from muscle injury. These effects were attributable to dysfunctional paracrine signaling mediated by extracellular vesicles (EVs) released from muscle cells. Specifically, we found that EVs collected from arsenic-exposed muscle constructs recapitulated the inhibitory effects of direct arsenic exposure on myofiber regeneration. In addition, muscle constructs treated with EVs isolated from muscles of arsenic-exposed mice displayed significantly decreased strength. Our findings highlight a novel model for muscle toxicity research and uncover a mechanism of arsenic-induced muscle dysfunction by the disruption of EV-mediated intercellular communication.
LAMA5 promotes cell proliferation and migration in ovarian cancer by activating Notch signaling pathway
Authors: Diao Bowen , Sun Chongfeng , Yu Panpan , Zhao Zouyu , Yang Ping 
Published: August 2023

AbstractLAMA5 (laminin α5) is a member of the laminin family. Despite the recent research implicating LAMA5 in cancer, the function of LAMA5 has remained uncertain in the progression of ovarian cancer (OC). Here, we investigated the functional influences of LAMA5 knockdown on OC in vitro and in vivo. In this study, we used immunohistochemistry (IHC) analysis to detect the relative expression of LAMA5 in OC and non‐cancer tissues, and we analyzed its connection with the overall survival (OS) of OC patients. To prove the role of LAMA5 in cell proliferation, migration, and invasion, LAMA5 expression in OC cell lines was inhibited by lentivirus. Compared with normal fallopian tube tissue, epithelial ovarian cancer (EOC) tissue showed critically higher LAMA5 expression levels; additionally, high LAMA5 levels were a poor predictor of OS. We found that cell progression was restrained in LAMA5‐knockdown OC cell lines in vivo and in vitro. Finally, LAMA5 might be a commanding inducer of the expression of epithelial‐mesenchymal transition (EMT) and Notch signaling pathway‐related markers. Together, our research indicates that LAMA5 is highly connected to OC progression as it may play a role in the EMT process through the Notch signaling pathway.
Learning Clinical Judgments With a Lens of Inclusivity
Authors: Monagle Janet , Roy Kaveri , Lasater Kathie 
Published: August 2023

Abstract
To prepare practice-ready graduates and promote NCLEX® success, many schools of nursing have adopted a clinical judgment model (CJM) to provide a framework for their curriculum and teaching strategies. Missing from most CJMs are clear principles of justice, equity, diversity, and inclusion (JEDI), imperative to prepare a nursing workforce to care holistically for diverse populations. This article describes the curriculum integration of an adapted model with added JEDI principles. By intentionally integrating the language and understanding of a CJM with JEDI principles, nurse educators can promote the clinical judgment development necessary to prepare graduates to care for diverse populations.
Readiness to Teach LGBTQ+ Health-Related Care
Authors: Dalbey Susan 
Published: August 2023

Abstract

AIM
The aim of this study was to explore nursing faculty’s readiness to teach lesbian, gay, bisexual, transgender, queer or another sexual or gender minority (LGBTQ+) health-related care.

BACKGROUND
Care of diverse populations is encouraged by nursing organizations. Assessing faculty’s readiness to teach this specific content is essential prior to educating student nurses.

METHOD
Walker and Avant’s eight-step approach was used to identify defining attributes, a model case, a borderline case, a contrary case, antecedents, consequences, empirical referents, and implications for nursing education.

RESULTS
Evaluation of the literature indicates nursing faculty’s readiness to teach LGBTQ+ health-related care would include defining attributes of teaching methodology, knowledge, attitude, experience, education, and comfort.

CONCLUSION
When faculty members demonstrate readiness to teach LGBTQ+ health-related care, students will learn and be able to provide such care as appropriate. Hopefully, this will result in better patient outcomes and individualized care for patients who identify as LGBTQ+.
First Metatarsal Hemiepiphysiodesis for the Treatment of Juvenile Hallux Valgus: A Systematic Review
Authors: Artioli Elena , Mazzotti Antonio , Langone Laura , Zielli Simone Ottavio , Arceri Alberto , Bonelli Simone , Faldini Cesare 
Published: August 2023

Introduction:Juvenile hallux valgus (JHV) is a pediatric deformity characterized by the varus deviation of the first metatarsal and valgus deviation of the proximal phalanx. Among the several surgical techniques available, hemiepiphysiodesis consists of the unilateral growth arrest of the first metatarsal physis. Despite this technique has been proposed over 70 years ago, only a few studies including clinical and radiological outcomes have been published, making the procedure unclear in terms of results. This systematic review aimed to evaluate the outcomes of hemiepiphysiodesis of the first metatarsal in the treatment of JHV.Methods:Google Scholar, Embase, PubMed, and Cochrane databases were searched for all the articles reporting on clinical and radiological results of hemiepiphysiodesis of the first metatarsal in the treatment of JHV. The selected articles were reviewed to extract demographic data, surgical techniques, complications, clinical outcomes, and radiological parameters.Results:Six articles were included in the qualitative analysis. A total of 85 patients with 144 halluces valgus were treated through hemiepiphysiodesis of the first metatarsal. The mean age at surgery was 10.7 years (range 5 to 15). The mean follow-up was 2.7 years (range 1 to 7.5). Hemiepiphysiodesis was performed through 2 different techniques. Eighteen (12.5%) complications occurred. The mean American Orthopaedic Foot and Ankle Society (AOFAS) score increased from 70.6 (range 49 to 93) preoperatively to 89.4 (range 72 to 100) postoperatively. The mean HVA improved from 28.3 (range 14 to 46) to 24.03 degrees (range 0 to 54), and the mean IMA improved from 13 (range 8 to 33) to 10.9 degrees (range 8 to 33).Conclusions:This review showed that hemiepiphysiodesis of the first metatarsal is a safe treatment for JHV. Improvement in both clinical and radiological results has been observed in all the studies, sometimes being statistically significant. Despite the improvement, mean postoperative radiological angles remained altered and consistent with mild-moderate hallux valgus. This suggests that hemiepiphysiodesis plays a bigger role in preventing the worsening of the deformity rather than correcting it. Randomized controlled trials with longer follow-up and a larger number of patients are needed to further investigate the efficacy and safety of this treatment.
Keloid Nodule Metabolic Activity for Continuous Expansion
Authors: Ueda Koichi , Lee Yuumi , Inomata Yosuke , Taniguchi Kohei , Uchiyama Kazuhisa , Ito Yuko 
Published: August 2023

Background:
We previously reported that keloid nodules had such specific structures that higher expression of autophagy proteins and glycolytic markers was observed in the central zone fibroblasts than in marginal zone fibroblasts. The purpose of this study is to investigate how keloid nodules play a role in metabolic activity for continuous expansion.

Methods:
A total of 57 nodules were randomly chosen from seven keloid samples and divided into four groups of disease duration (2, 4, 6, and 17 years). Immunohistochemical and immunofluorescent analyses were performed.

Results:
Immunohistochemical analysis with anti-CD-31 confirmed that the nodules had a structure with a greater number of vessels in the marginal zone than in the central zone. The density of fibroblasts in nodules (190.29 ± 64.45) was significantly higher than that of surrounding connective tissue (140.18 ± 63.94) (P < 0.05).
The area of each nodule increased for 2 to 4 years, then decreased, graphically represented by an approximately horizontal line, to 17 years. The ratio of total nodule area/dermis area increased as disease duration lengthened. The maximum ratio was the 17-year group at 48.01%. The nodule number/dermis area ratio rose approximately with advancing disease duration.

Conclusions:
Instead of increasing the size of the nodules, their number and total area increased within the keloid lesions. We believe that the keloid nodules must play an important role in energy metabolic activity for continuous growth by increasing in number and total area.
RECALCITRANT FUSARIUM FUNGAL ENDOPHTHALMITIS: NOVEL SURGICAL TECHNIQUE COMBINING VITRECTOMY WITH INTRALESIONAL INJECTION OF VORICONAZOLE AND POVIDONE-IODINE
Authors: Malbin Brett , Guest John-Michael , Lin Xihui 
Published: August 2023

Purpose:
To show the feasibility while comparing functional and anatomical outcomes of vitrectomy combined with intralesional injection of voriconazole and povidone-iodine for recalcitrant Fusarium fungal endophthalmitis.

Methods:
In a noncomparative interventional case, vitrectomy combined with intralesional injection of voriconazole and povidone-iodine was performed in a single patient.

Results:
Following vitrectomy and intralesional injection, our patient's fungal endophthalmitis stabilized and vision improved to 20/25. He did not require further intravitreal injections or vitrectomy.

Conclusion:
Vitrectomy and intralesional injection for the treatment of refractory filamentary fungal endophthalmitis proved to be safe and effective for this case.
Occupational Evaluation of Federal Highway Police Officers Exposed to Cholinesterase Inhibiting Insecticides
Authors: Bonache Juliandra Spagnol , Costa Bruna Francisquete , Hoshino Marcio Tadashi , Ludwig Larissa , Barboza de Oliveira Marcos Aurélio , Emerick Guilherme Luz 
Published: August 2023

Objective
The aim of this study is to evaluate the effects of occupational exposure of federal highway police (PRF) officers to cholinesterase-inhibiting insecticides.

Methods
We evaluate erythrocyte and plasma cholinesterase activity, pulse rate, systolic and diastolic pressure, and clinical evaluation through the Mini-Mental State Examination and the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition.

Results
All PRF officers evaluated were male, between 22 and 49 years of age. Pulse rate of the subjects were statistically superior in the post-exposure moment when compared with pre-exposure moment. Inhibition of acetylcholinesterase and butyrylcholinesterase was significant in the post-exposure moment when compared with pre-exposure moment.

Conclusions
The results of the present work show that there are significant biochemical changes, which can be the beginning of serious deleterious effects to the health of PRF officers.
Association Between Weight Reduction and Employees’ Healthcare Cost
Authors: Peasah Samuel K. , Drnach Alek , Rosenberg Eric , Good Chester B. 
Published: August 2023

Objective
The aim of the study is to assess the impact of ≥15% body mass index (BMI) reduction on employees’ health expenditures.

Methods
We retrospectively analyzed health risk assessment surveys combined with insurance claims from January 2014 to December 2019. We compared costs of employees with baseline BMI > 30 who reported ≥15% BMI reduction in subsequent health risk assessment reports with employees who lost ≤5% BMI within the same period, matching the two cohorts on demographics and costs.

Results
The study cohort of 197 lost an average of 23% of their BMI from baseline. The average age was 44 years with majority females (approximately 80%). Group health insurance payments were similar at baseline; at year 1, the study cohort had a 33% payment reduction compared with 10% reduction in the control group.

Conclusions
A ≥15% BMI reduction was associated with a substantial medical cost savings.
Perioperative and oncologic outcomes of minimally-invasive surgery for renal cell carcinoma with venous tumor thrombus: a systematic review and meta-analysis of comparative trials
Authors: Li Kun-peng , Chen Si-yu , Wang Chen-yang , Li Xiao-ran , Yang Li 
Published: August 2023

Background:
The present study aimed to conduct a pooled analysis to compare the perioperative and oncologic outcomes of minimally-invasive radical nephrectomy with tumor thrombus (MI-RNTT) with open radical nephrectomy with tumor thrombus (O-RNTT).

Methods:
This study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Four electronic databases (PubMed, Embase, Web of Science, and the Cochrane Library database) were systematically searched to identify relevant studies published in English up to December 2022. The primary outcomes were perioperative results, complications, and oncologic outcomes. Review Manager 5.4 was used for this analysis.

Results:
In total, eight retrospective trials with a total of 563 patients were included. Compared to O-RNTT, MI-RNTT had shorter hospitalization time [weighted mean difference (WMD) -3.58 days, 95% CI: −4.56 to −2.59; P<0.00001), lower volumes of blood loss (WMD -663.32 ml, 95% CI: −822.22 to −504.42; P<0.00001), fewer transfusion rates (OR 0.18, 95% CI: 0.09–0.35; P<0.00001), fewer overall complications (OR 0.33, 95% CI: 0.22–0.49; P<0.00001), and fewer major complications s (OR 0.49, 95% CI: 0.24–1.00; P=0.05). However, operative time, intraoperative complications, mortality rate (intraoperative, within 30 days, and total mortality), overall survival, recurrence-free survival, and cancer-specific survival did not significantly differ between the two groups.

Conclusions:
MI-RNTT possesses more benefits than O-RNTT in terms of length of hospital stay, blood loss, and complications and provides comparable mortality rates and oncologic outcomes. However, more comprehensive and rigorous research is warranted to further validate the outcomes, which should include a larger sample size and comprehensive data from high-volume medical centers.
Infantile Myofibromatosis With Cutaneous, Visceral, and CNS Involvement: A Multimodal Approach to Therapy
Authors: Gehle Stephanie , Quinsey Carolyn , McShane Diana , Thompson Patrick , Quezada Gerardo 
Published: August 2023

Background:
Infantile myofibromatosis (IM) is a rare benign tumor of infancy. Cases with solitary and multicentric disease usually spontaneously regress, but multicentric disease with visceral involvement carries a poor prognosis. Few cases of multicentric disease with central nervous system (CNS) involvement have been reported, and none report survival.

Observations:
We present a newborn with multicentric IM with cutaneous, visceral, and CNS involvement. She was treated with vinblastine, methotrexate, and the novel addition of intrathecal methotrexate with treatment response after 1 year of therapy.

Conclusions:
Multicentric IM with CNS involvement can be successfully treated with a multimodal approach of chemotherapy with the addition of intrathecal methotrexate and surgery.
Sarcopenia in Children With Wilms Tumor: A Marker of Undernutrition Which May Impact Adversely on Clinical Outcomes
Authors: de Oliveira Wilson E. , Murra Mariana S. , Tufi Leticia M.B. , Cavalcante Carlos E.B. , de Oliveira Marco A. , da Costa Ricardo F.A. , Rosa Bianca R. , da Silva Roberta Z.M. , Ribeiro Rodrigo C. , Ladas Elena J. , Barr Ronald D. 
Published: August 2023

The therapeutic approach to Wilms tumor (WT) is multidisciplinary and leads to significant patient impairment, increasing the risk of nutritional compromise and malnutrition. Children with cancer are vulnerable to sarcopenia which has been recognized as a negative impact of anticancer therapy. Recent studies have highlighted the reduction in the total psoas muscle area (TPMA) to be associated with a poor prognosis in many pediatric diseases, including cancer. This study aims to evaluate changes in the TPMA compartment during the treatment of children with WT. An observational, longitudinal, and retrospective study was undertaken in a single institution evaluating children (1 to 14 y, n=38) with WT between 2014 and 2020. TPMA was assessed by the analysis of previously collected, electronically stored computed tomography images of the abdomen obtained at 3 time points: diagnosis, preoperatively, and 1 year after surgery. For all patients, TPMA/age were calculated with a specific online calculator. Our data show a high incidence of sarcopenia (55.3%) at diagnosis which increased after 4 to 6 weeks of neoadjuvant chemotherapy (73.7%) and remained high (78.9%) 1 year after the surgical procedure. Using TPMA/age Z-score curves we have found significant and rapid muscle loss in children with WT, with little or no recovery in the study period.
Detection and Estimation of Age of Injuries in Fresh Bodies Using Immunohistochemistry
Authors: Ramamurthy Kokila , Sundaram Sandhya , Sivaramakrishnan Sneha , Paranthaman Sampath Kumar , Balasubramanian Archana , Pradhan Priyadarshee 
Published: August 2023

Abstract
A cross-sectional study of the wound age estimation from the forensic skin wound samples was performed immunohistochemically with a sample size of 40 (n = 40). The samples were segregated according to the appropriate stages of wound healing with the help of hematoxylin-eosin staining. Later, they were subjected to immunohistochemistry staining with anti–AQP3 antibody. Quantification of the expression of AQP3 in the injured and uninjured formalin-fixed skin tissues was done semiquantitatively and manually under 400× magnifications. The AQP3-positive cells were correlated with the duration of injury, and the results were statistically analyzed. More AQP3 expressions were found in the proliferative phase than the inflammatory and maturation phase of wound healing. Neither the diversity in age group nor the sex differentiation showed any specific correlation with the expression of keratinocytic aquaporin cells. Likewise, parameters such as the type of injury, mode of injury, and the postmortem interval also did not show any significant relationship with the expression of the aquaporin positivity. Thus, it is revealed that skin wounds between 5 and 10 days expressed aquaporin cell numbers of more than 300. Hence AQP3 estimation helps in determining the time since injury with a more accuracy.
Single-cell analyses of CSF and PBMCs from anti-NMDAR encephalitis patients reveals distinct characteristics of T cell subpopulations
Authors: Li Sisi , Hu Xiang , Yang Yi , Wang Jierui , Hong Zhen , Zhou Dong , Li Jinmei 
Published: August 2023

AbstractBackgroundAnti-N-methyl-D-aspartate receptor encephalitis (NMDAR-E) is a common and severe antibody-mediated autoimmune encephalitis. While the roles of B cells and NMDAR antibodies in NMDAR-E have been extensively studied, the involvement of T cell subpopulations in the disease progression remains unclear.MethodsThis study conducted single-cell RNA sequencing, single-cell TCR sequencing, and flow cytometry to analyze the T cell subpopulations and their transcriptomic characteristics in NMDAR-E patients and control individuals. Furthermore, it explored the interaction between CD8+T cells and B cells through in vitro cell co-culture and cell communication analysis.ResultsThe study found activated CD8+T cell subpopulations in the cerebrospinal fluid (CSF) and peripheral blood mononuclear cells (PBMCs) of NMDAR-E patients, with some subpopulations exhibiting significant TCR clonal expansion. Differential expression gene analysis revealed upregulation of genes related to cytotoxicity, tissue residency, Th1, IFN, or TCR signaling in certain activated CD8+T cell and CD4+memory T cell subpopulations. In vitro co-culture experiments demonstrated that CD8+T cells from the PBMCs of NMDAR-E patients could induce apoptosis of their own B cells. Cell interaction analysis revealed the existence of interactions between KIR+CD8+T cells and B cell subpopulations in NMDAR-E patients.ConclusionThis study explored the changes and transcriptomic characteristics of activated CD8+T cell subpopulations in the CSF and PBMCs of NMDAR-E patients. Additionally, it discovered the impact of CD8+T cells from NMDAR-E patients on their own B cells, providing new evidence for the interaction between CD8+T cells and B cells.
Classifying autism in a clinical population based on motion synchrony: a proof-of-concept study using real-life diagnostic interviews
Authors: Koehler Jana Christina , Dong Mark Sen , Song Da-Yea , Bong Guiyoung , Koutsouleris Nikolaos , Yoo Heejeong , Falter-Wagner Christine M. 
Published: August 2023

AbstractPredictive modeling strategies are increasingly studied as a means to overcome clinical bottlenecks in the diagnostic classification of autism spectrum disorder. However, while some findings are promising in the light of diagnostic marker research, many of these approaches lack the scalability for adequate and effective translation to everyday clinical practice. In this study, our aim was to explore the use of objective computer vision video analysis of real-world autism diagnostic interviews in a clinical sample of children and adolescents to predict diagnosis. Specifically, we trained a support vector machine learning model on interpersonal synchrony data recorded in Autism Diagnostic Observation Schedule (ADOS) interviews of patient-clinician dyads. Our model was able to classify dyads involving an autistic patient (n=56) with a balanced accuracy of 63.4% against dyads including a patient with other psychiatric diagnoses (n=38). Further analyses revealed no significant associations between our classification metrics with clinical ratings. We argue that, given the above-chance performance of our classifier in a highly heterogeneous sample both in age and diagnosis, with few adjustments this highly scalable approach presents a viable route for future diagnostic marker research in autism.