bluuebunny/crossref_metadata_2025_split huggingface dataset:
PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity
Authors: Nakamura Koki , Aoyama-Ishiwatari Saeko , Nagao Takahiro , Paaran Mohammadreza , Obara Christopher J. , Sakurai-Saito Yui , Johnston Jake , Du Yudan , Suga Shogo , Tsuboi Masafumi , Nakakido Makoto , Tsumoto Kouhei , Kishi Yusuke , Gotoh Yukiko , Kwak Chulhwan , Rhee Hyun-Woo , Seo Jeong Kon , Kosako Hidetaka , Potter Clint , Carragher Bridget , Lippincott-Schwartz Jennifer , Polleux Franck , Hirabayashi Yusuke 
Published: August 2023

AbstractMitochondria-ER membrane contact sites (MERCS) represent a fundamental ultrastructural feature underlying unique biochemistry and physiology in eukaryotic cells. The ER protein PDZD8 is required for the formation of MERCS in many cell types, however, its tethering partner on the outer mitochondrial membrane (OMM) is currently unknown. Here we identified the OMM protein FKBP8 as the tethering partner of PDZD8 using a combination of unbiased proximity proteomics, CRISPR-Cas9 endogenous protein tagging, Cryo-Electron Microscopy (Cryo-EM) tomography, and correlative light-EM (CLEM). Single molecule tracking revealed highly dynamic diffusion properties of PDZD8 along the ER membrane with significant pauses and capture at MERCS. Overexpression of FKBP8 was sufficient to narrow the ER-OMM distance, whereas independent versus combined deletions of these two proteins demonstrated their interdependence for MERCS formation. Furthermore, PDZD8 enhances mitochondrial complexity in a FKBP8-dependent manner. Our results identify a novel ER-mitochondria tethering complex that regulates mitochondrial morphology in mammalian cells.
Jasmonates regulate auxin-mediated root growth inhibition in response to rhizospheric pH in Arabidopsis
Authors: Singh Ajit Pal , Kanwar Rajni , Pandey Ajay K. 
Published: August 2023

AbstractRhizospheric pH severely impacts plant growth and fitness through a numerous process and has emerged as a major determinant of crop productivity. Despite numerous attempts, the key questions related to plants response against rhizospheric pH remains largely elusive. The present study provides a mechanistic framework for rhizospheric pH-mediated root growth inhibition (RGI). Utilizing various genetic resources combined with pharmacological agents and high-resolution confocal microscopy, the study provides direct evidences for the involvement of jasmonates and auxin in rhizospheric pH-mediated RGI. We show that auxin maxima at root tip is tightly regulated by the rhizospheric pH. In contrast, jasmonates (JAs) abundance inversely correlates with rhizospheric pH. Further, JA mediated regulation of auxin maxima byGRETCHEN HAGEN 3(GH3) family genes explains the pattern of RGI observed over the entire range of rhizospheric pH. Our findings revealed auxin as the key regulator of RGI during severe pH conditions, while JAs antagonistically regulate auxin response against rhizospheric pH.HighlightThe current study identifies the mechanistic framework of rhizospheric pH mediated root growth inhibition in model plant Arabidopsis through a prominent crosstalk between two phytohormones i.e. auxin and jasmonates.
Parallel, Continuous Monitoring and Quantification of Programmed Cell Death in Plant Tissue
Authors: Collins Alexander Silva Pinto , Kurt Hasan , Duggan Cian , Cotur Yasin , Coatsworth Philip , Naik Atharv , Kaisti Matti , Bozkurt Tolga , Güder Firat 
Published: August 2023

AbstractThe accurate quantification of hypersensitive response (HR) programmed cell death is imperative for understanding plant defense mechanisms and developing disease-resistant crop varieties. In this study, we report an accelerated phenotyping platform for the continuous-time, rapid and quantitative assessment of HR: Parallel Automated Spectroscopy Tool for Electrolyte Leakage (PASTEL). Compared to traditional HR assays, PASTEL significantly improves temporal resolution and has high sensitivity, facilitating the detection of microscopic levels of cell death. We validated PASTEL by transiently expressing the effector protein AVRblb2 in transgenic lines of the model plantNicotiana benthamiana(expressing the corresponding resistance protein Rpi-blb2) to reliably induce HR. We were able to detect cell death at microscopic intensities, where leaf tissue appeared healthy to the naked eye one week after infiltration. PASTEL produces large amounts of frequency domain impedance data captured continuously (sub-seconds to minutes). Using this data, we developed a supervised machine learning models for classification of HR. We were able to classify input data (inclusive of our entire tested concentration range) as HR-positive or negative with 84.1% mean accuracy (F1score = 0.75) at 1 hour and with 87.8% mean accuracy (F1score = 0.81) at 22 hours. With PASTEL and the ML models produced in this work, it is possible to phenotype disease resistance in plants in hours instead of days to weeks.
Logistics 4.0 for supply chain performance: perspectives from a retailing case study
Authors: Hrouga Mustapha , Sbihi Abdelkader 
Published: August 2023

PurposeThis study considers the potential of logistics 4.0 for supply chain (SC) optimization in French retail. The authors investigate the implementation of Industry 4.0 technologies to optimize SC performance in the retail sector and SC's role in the digital transformation in supply chain management (SCM).Design/methodology/approach The authors first carry out a comprehensive bibliographic taxonomy to highlight the different existing digital tools. Based on this, the authors posed three research questions (RQs) and hypotheses to examine the contribution of logistics 4.0 in improving the performance of retail logistics. Then, the authors considered a case study of retail in France based on qualitative and quantitative analysis to answer all the RQs and examine the hypotheses.FindingsThe results showed that digital tools such as Cyber Security Systems (CSS), Big Data Analytics (BDA) and Blockchain (BC) technology are the most effective and appropriate tools to optimize the SC performance in retail.Practical implicationsThis research work showed that the implementation of these tools in retail can offer several benefits such as improved productivity, optimized delivery times, improved inventory management and secure real-time communication, which leads to improved profitability of the SC.Originality/valueThe study opens a door to develop practical roadmaps for companies that enable smart deliveries based on logistics 4.0.
Strategic entrepreneurial behaviors and firm performance: the moderating role of narcissism and Machiavellianism
Authors: Shirokova Galina , Galieva Nailya , Doktorova Diana , White Joshua V. , Marino Louis 
Published: August 2023

PurposeThis study examines the relationship between strategic entrepreneurial behaviors (SEBs) and the performance of small and medium-sized enterprises (SMEs) in an emerging market context. The authors expand upon prior work in this area by building and testing a model that assesses the moderating effect of CEOs’ narcissism and Machiavellianism on the relationship between SEBs and SME performance.Design/methodology/approachTo test the authors’ theoretical model, the authors use the results of a larger data collection project in Russia to create a national random sample of 372 Russian SMEs that were approached between August and November 2019.FindingsThe authors found support for the positive relationship between SEBs and SME performance. Additionally, the authors found that CEO narcissism and Machiavellianism strengthen the relationship between SEBs and firm performance.Originality/valueThis study is an important step toward enriching the understanding of the role of CEO personality traits in shaping the efficiency of entrepreneurial behavior at the firm level. Extending previous research, the authors show that SEBs have a positive effect on firm performance in an emerging market context. Additionally, the authors contribute insight about how personality characteristics of CEOs, specifically narcissism and Machiavellianism, influence the relationship between entrepreneurial behavior and firm performance. Finally, the authors’ research contributes to the development of strategic leadership theory: the results offer insight to scholars regarding the potentially beneficial attributes of otherwise “dark” leaders.
Dermatophyte antigen kit: A useful diagnostic tool for onychomycosis
Authors: Noguchi Hiromitsu , Matsumoto Tadahiko , Mimura Yoshihiro , Kubo Masahide , Higuchi Sara , Kashiwada‐Nakamura Kayo , Kimura Utako , Hiruma Masataro , Fukushima Satoshi 
Published: August 2023

AbstractA dermatophyte antigen kit (DQT) was released in Japan as an in vitro diagnostic tool to identify tinea unguium in June 2022. From July 2022 to February 2023, we examined 75 potassium hydroxide (KOH)‐negative patients (male, n = 23; female, n = 52; mean ± SD age, 63.6 ± 13.9 years) and determined the accuracy in confirming the fungal element with ZoomBlue™ staining at 400× magnification. The DQT results were classified into three categories. DQT‐positive onychomycosis was detected in 27 patients with tinea unguium and two with non‐dermatophyte onychomycosis. Fungal cultures were positive in 14 (51.8%) patients (Trichophyton rubrum [n = 11], T. interdigitale [n = 1], Fusarium solani [n = 1], and Talaromyces muroii [n = 1]). DQT‐negative onychomycosis included ten patients with cured tinea unguium and 3 with Candida onychomycosis. Twenty‐three patients had DQT‐negative mimics for onychomycosis (onychauxis [n = 11], traumatic onycholysis [n = 8], yellow nail syndrome [n = 5], pincer nail deformity [n = 3], brittle nail syndrome [n = 2], contact dermatitis [n = 2], lichen planus [n = 1] and psoriasis [n = 1]). Because sparse, atrophic and/or fragmented mycelia are invisible in direct microscopy with potassium hydroxide (KOH) at 100× magnification, DQT was beneficial for diagnosing onychomycosis.
Low‐cost management interventions and their impact on multilevel trade‐offs in agricultural grasslands
Authors: Burian Alfred , Norton Briony A. , Alston Debbie , Willmot Alan , Reynolds Sarah , Meynell Godfrey , Lynch Paul , Bulling Mark 
Published: August 2023

Abstract

Cost‐effective strategies to increase biodiversity are a fundamental requirement to reconcile conservation and food production in agricultural landscapes. Key for the implementation of such strategies is an accurate quantification of both their benefits and potential associated trade‐offs.

We therefore assessed, in a commercially managed grassland, biodiversity responses to two low‐cost management interventions and their mediating effects on ecosystem services.

In a 6‐year experiment, we showed that a one‐time seed bank activation treatment had strong initial impacts on biodiversity, increasing plant richness in year 1 by 61%. Long‐term effects, which were also driven by the second management intervention, the propagation of the keystone species yellow rattle, were weaker but nonetheless substantial. These positive biodiversity responses improved ecosystem multifunctionality through additive positive effects of richness, evenness and phylogenetic distinctiveness on nectar production and structural habitat complexity.

In contrast, hay biomass production was negatively affected by both management interventions, resulting in a multilevel trade‐off between biomass production competing with biodiversity conservation and the provisioning of other ecosystem services.

Synthesis and applications. In this study, we demonstrate that the maximisation of either biodiversity or biomass production requires largely different land management practices. The evaluation of this trade‐off, however, is strongly dependent on its social, economic and ecological context and requires clearly defined land management priorities for both food production and biodiversity conservation.
Estimating state‐specific population‐based hospitalization rates from in‐state hospital discharge data
Authors: Roemer Marc , Schaefer Mary Beth , Pickens Gary T. , Barrett Marguerite L. 
Published: August 2023

AbstractObjectiveTo develop weights to estimate state population‐based hospitalization rates for all residents of a state using only data from in‐state hospitals which exclude residents treated in other states.Data Sources and Study SettingAgency for Healthcare Research and Quality, Healthcare Cost and Utilization Project (HCUP), State Inpatient Databases (SID), 2018–2019, 47 states+DC.Study DesignWe identified characteristics for patients hospitalized in each state differentiating movers (discharges for patients hospitalized outside state of residence) from stayers (discharges for patients hospitalized in state of residence) and created weights based on 2018 data informed by these characteristics. We calculated standard errors using a sampling framework and compared weight‐based estimates against complete observed values for 2019.Data Collection/Extraction MethodsSID are based on administrative billing records collected by hospitals, shared with statewide data organizations, and provided to HCUP.Principal FindingsOf 34,186,766 discharged patients in 2018, 4.2% were movers. A higher share of movers (vs. stayers) lived in state border and rural counties; a lower share had discharges billed to Medicaid or were hospitalized for maternal/neonatal services. The difference between 2019 observed and estimated total discharges for all included states and DC was 9402 (mean absolute percentage error = 0.2%). We overestimated discharges with an expected payer of Medicaid, from the lowest income communities, and for maternal/neonatal care. We underestimated discharges with an expected payer of private insurance, from the highest income communities, and with injury diagnoses and surgical services. Estimates for most subsets were not within a 95% confidence interval, likely due to factors impossible to account for (e.g., hospital closures/openings, shifting consumer preferences).ConclusionsThe weights offer a practical solution for researchers with access to only a single state's data to account for movers when calculating population‐based hospitalization rates.
The valuation of gross ecosystem product in the three provinces in northeast of China
Authors: Fan Yu , Ji Jiayang , Jia Changgeng , Lei Ming , Wu Wenjun , Zheng Yun , Wang Zichao , Zhang Guohui , Song Youtao 
Published: August 2023

AbstractAchieving sustainable development is an issue of global concern. Accounting for the gross ecosystem product (GEP) value can specifically quantify the value of ecosystems for people, which is conducive to the formulation of sustainable management decisions. We have improved the GEP framework accounting method for the first time and evaluated the ecosystem service value of 36 cities in three provinces in the northeast (TPN) of China from 2000 to 2019, which provides decision‐making references for the sustainable development of TPN. The main accounting results and policy recommendations are as follows: (1) The ecological value of Northeast China is in an upward stage—GEP has increased from 3.1 trillion yuan in 2000 to 4.5 trillion yuan in 2019, with climate regulation and water conservation functions being the main driving factors for GEP. (2) Based on the dual analysis of GEP and gross domestic product (GDP), there is still high room for improvement in most cities in the TPN region. While ensuring the protection of ecosystem services, cities can convert ecological value into economic value through carbon trading, water rights trading, ecological compensation, and other methods based on ecological characteristics, which is conducive to the sustainable development of GDP and GEP in the TPN region in the future. (3) The cold and hot spot analysis of GEP also provides decision‐making basis for the planning of future ecosystem service protection areas in the TPN region.
Physiological response mechanism of heavy metal‐resistant endophytic fungi isolated from the roots of Polygonatum kingianum
Authors: Cao Guan‐Hua , Li Xiao‐Gang , Zhang Chen‐Rui , Xiong Yi‐Ran , Li Xue , Li Tong , He Sen , Cui Zheng‐Guo , Yu Jie 
Published: August 2023

AbstractThis study aims to evaluate the tolerance of endophytic fungi isolated from the fibrous roots of Polygonatum kingianum to arsenic (As) and cadmium (Cd) and their physiological response mechanisms. Five isolated strains were obtained with EC50 values for As(V) ranging from 421 to 1281 mg/L, while the other three strains tolerated Cd(II) with an EC50 range of 407–1112 mg/L. Morphological and molecular identification indicated that these eight strains were Cladosporium spp. belonging to dark septate endophytes (DSEs). The contents of metal ions in mycelium sharply increased, reaching 38.87 mg/kg for strain MZ‐11 under As(V) stress and 0.33 mg/kg for fungus PR‐2 under Cd(II). The physiological response revealed that the biomass decreased with increasing concentrations of As(V) or Cd(II), and the activity of superoxide dismutase significantly improved under the corresponding EC50‐concentration As/Cd of the strains, as well as the contents of antioxidant substances, including metallothionein, glutathione, malondialdehyde, melanin, and proline. Taken together, the filamentous fungi of Cladosporium spp. accounted for a high proportion of fungi isolated from the fibrous roots of P. kingianum and had a strong capacity to tolerate As(V) or Cd(II) stress by improving antioxidase activities and the content of antioxidant substances, and immobilization of metal ions in hyphae.
Endodontic access cavities: Fears and difficulties of Moroccan dental students related to teachers, self‐confidence and technical protocol
Authors: Ghoul Sonia , Faridi Fatima‐Ezzahra , Haj Khalaf Louzan , Razine Rachid , Sikkou Khadija , Idrissi‐Kaitouni Lamia , Rahhali Mohamed , Sakout Majid 
Published: August 2023

AbstractFears and difficulties of Moroccan dental students regarding endodontic access cavity were evaluated among 3 items: (i) teachers, (ii) student confidence and (iii) access cavity protocol. A three Likert scale was used to rate 24 statements. Specific scores between 1 and 3 and mean scores between 0 and 1 were used to assess statements and items, respectively. Among 116 students, the mean score of difficulty related to teachers (0.48 ± 0.14) was significantly low (p < 0.001) compared to student confidence (0.69 ± 0.12) and cavity protocol (0.68 ± 0.08). The number of teachers was esteemed insufficient, additional training sessions were requested and maxillary teeth and molars were rated the most difficult with specific scores of 1.91 ± 0.80, 2.72 ± 0.61, 2.22 ± 0.67 and 2.47 ± 0.74, respectively. The highest specific score referred to the risk of making ledges (2.8 ± 0.56) or perforations (2.76 ± 0.59). Students expressed a lack of confidence, difficulty with upper molars and fears of making procedural errors.
The influence of air pollution on gestational age at delivery and birthweight in patients with or without respiratory allergy: A nested case–control study
Authors: Bartha Irene , De La Fuente Miguel , Martinez‐Sanchez Nuria , De La Calle María , Martin Boado Elena , Bartha José Luis 
Published: August 2023

AbstractIntroductionAir pollution is a current major health issue. The burden of airborne pollutants and aeroallergen levels varies throughout the year, as well as their interaction and consequences. Prenatal exposure during pregnancy has been associated with adverse perinatal outcomes. The aim of this study was to evaluate the impact of air pollutants on perinatal outcomes in patients with or without respiratory allergy.Material and methodsNested case–control retrospective study on 3006 pregnant women. Correlations between concentrations of common pollutants in each trimester of pregnancy and on average during the whole pregnancy and both gestational age at delivery and birthweight were studied. Pearson's correlation coefficient and binary logistic regression were used.ResultsIn general, pollutants correlated more strongly with birthweight than with gestational age at delivery. Nine‐month NO2, SO2, CO, and benzene, and second‐trimester CO negatively correlated with birthweight, whereas only first‐trimester NO2 showed a very mild correlation with gestational age at delivery. Negative correlations between pollutants and birthweight were much stronger in the respiratory allergy group (n = 43; 1.4%) than in the non‐allergic group. After adjustments, the most significant predictive pollutant of birthweight was SO2 in both groups. The best predictive model was much stronger in the allergic group for third‐trimester SO2 (R2 = 0.12, p = 0.02) than in the non‐allergic group for total SO2 (R2 = 0.002, p = 0.02). For each unit that SO2 increased, birthweight reduced by 3.22% vs. 1.28% in each group, respectively.ConclusionsAir pollutant concentrations, especially SO2, negatively influenced birthweight. The impact of this association was much stronger and more relevant in the group of women with respiratory allergies.
Abnormal myosin post‐translational modifications and ATP turnover time associated with human congenital myopathy‐related RYR1 mutations
Authors: Sonne Alexander , Antonovic Anna Katarina , Melhedegaard Elise , Akter Fariha , Andersen Jesper L. , Jungbluth Heinz , Witting Nanna , Vissing John , Zanoteli Edmar , Fornili Arianna , Ochala Julien 
Published: August 2023

AbstractAimConditions related to mutations in the gene encoding the skeletal muscle ryanodine receptor 1 (RYR1) are genetic muscle disorders and include congenital myopathies with permanent weakness, as well as episodic phenotypes such as rhabdomyolysis/myalgia. Although RYR1 dysfunction is the primary mechanism in RYR1‐related disorders, other downstream pathogenic events are less well understood and may include a secondary remodeling of major contractile proteins. Hence, in the present study, we aimed to investigate whether congenital myopathy‐related RYR1 mutations alter the regulation of the most abundant contractile protein, myosin.MethodsWe used skeletal muscle tissues from five patients with RYR1‐related congenital myopathy and compared those with five controls and five patients with RYR1‐related rhabdomyolysis/myalgia. We then defined post‐translational modifications on myosin heavy chains (MyHCs) using LC/MS. In parallel, we determined myosin relaxed states using Mant‐ATP chase experiments and performed molecular dynamics (MD) simulations.ResultsLC/MS revealed two additional phosphorylations (Thr1309‐P and Ser1362‐P) and one acetylation (Lys1410‐Ac) on the β/slow MyHC of patients with congenital myopathy. This method also identified six acetylations that were lacking on MyHC type IIa of these patients (Lys35‐Ac, Lys663‐Ac, Lys763‐Ac, Lys1171‐Ac, Lys1360‐Ac, and Lys1733‐Ac). MD simulations suggest that modifying myosin Ser1362 impacts the protein structure and dynamics. Finally, Mant‐ATP chase experiments showed a faster ATP turnover time of myosin heads in the disordered–relaxed conformation.ConclusionsAltogether, our results suggest that RYR1 mutations have secondary negative consequences on myosin structure and function, likely contributing to the congenital myopathic phenotype.