bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the functions of Stromal Interaction Molecule-1 in amelogenesis using AmelX-iCre mice
Authors: Said Raed , Mortazavi Helyasadat , Cooper David , Ovens Katie , McQuillan Ian , Papagerakis Silvana , Papagerakis Petros 
Published: March 2023

Introduction: The intracellular Ca2+ sensor stromal interaction molecule 1 (STIM1) is thought to play a critical role in enamel development, as its mutations cause Amelogenesis Imperfecta (AI). We recently established an ameloblast-specific (AmelX-iCre) Stim1 conditional deletion mouse model to investigate the role of STIM1 in controlling ameloblast function and differentiation in vivo (Stim1 cKO). Our pilot data (Said et al., J. Dent. Res., 2019, 98, 1002–1010) support our hypothesis for a broad role of Stim1 in amelogenesis. This paper aims to provide an in-depth characterization of the enamel phenotype observed in our Stim1 cKO model.Methods: We crossed AmelX-iCre mice with Stim1-floxed animals to develop ameloblast-specific Stim1 cKO mice. Scanning electron microscopy, energy dispersive spectroscopy, and micro- CT were used to study the enamel phenotype. RNAseq and RT-qPCR were utilized to evaluate changes in the gene expression of several key ameloblast genes. Immunohistochemistry was used to detect the amelogenin, matrix metalloprotease 20 and kallikrein 4 proteins in ameloblasts.Results: Stim1 cKO animals exhibited a hypomineralized AI phenotype, with reduced enamel volume, diminished mineral density, and lower calcium content. The mutant enamel phenotype was more severe in older Stim1 cKO mice compared to younger ones and changes in enamel volume and mineral content were more pronounced in incisors compared to molars. Exploratory RNAseq analysis of incisors’ ameloblasts suggested that ablation of Stim1 altered the expression levels of several genes encoding enamel matrix proteins which were confirmed by subsequent RT-qPCR. On the other hand, RT-qPCR analysis of molars’ ameloblasts showed non-significant differences in the expression levels of enamel matrix genes between control and Stim1-deficient cells. Moreover, gene expression analysis of incisors’ and molars’ ameloblasts showed that Stim1 ablation caused changes in the expression levels of several genes associated with calcium transport and mitochondrial kinetics.Conclusions: Collectively, these findings suggest that the loss of Stim1 in ameloblasts may impact enamel mineralization and ameloblast gene expression.
The clinical, neuropsychological, and brain functional characteristics of the ADHD restrictive inattentive presentation
Authors: Wu Zhao-Min , Wang Peng , Liu Juan , Liu Lu , Cao Xiao-Lan , Sun Li , Yang Li , Cao Qing-Jiu , Wang Yu-Feng , Yang Bin-Rang 
Published: March 2023

ObjectivesThere is an ongoing debate about the restrictive inattentive (RI) presentation of attention deficit hyperactivity disorder (ADHD). The current study aimed to systematically investigate the clinical, neuropsychological, and brain functional characteristics of children with ADHD restrictive inattentive presentation.MethodsA clinical sample of 789 children with or without ADHD participated in the current study and finished clinical interviews, questionnaires, and neuropsychological tests. Those individuals with a diagnosis of ADHD were further divided into three subgroups according to the presentation of inattentive and/or hyperactive/impulsive symptoms, the ADHD-RI, the ADHD-I (inattentive), and the ADHD-C (combined) groups. Between-group comparisons were carried out on each clinical and neuropsychological measure using ANCOVA, with age and sex as covariates. Bonferroni corrections were applied to correct for multiple comparisons. Two hundred twenty-seven of the subjects also went through resting-state functional magnetic resonance imaging scans. Five ADHD-related brain functional networks, including the default mode network (DMN), the dorsal attention network (DAN), the ventral attention network, the executive control network, and the salience network, were built using predefined regions of interest (ROIs). Voxel-based group-wise comparisons were performed.ResultsCompared with healthy controls, all ADHD groups presented more clinical problems and weaker cognitive function. Among the ADHD groups, the ADHD-C group had the most clinical problems, especially delinquent and aggressive behaviors. Regarding cognitive function, the ADHD-RI group displayed the most impaired sustained attention, and the ADHD-C group had the worst response inhibition function. In terms of brain functional connectivity (FC), reduced FC in the DMN was identified in the ADHD-C and the ADHD-I groups but not the ADHD-RI group, compared to the healthy controls. Subjects with ADHD-I also presented decreased FC in the DAN in contrast to the control group. The ADHD-RI displayed marginally significantly lower FC in the salience network compared to the ADHD-I and the control groups.ConclusionThe ADHD-RI group is distinguishable from the ADHD-I and the ADHD-C groups. It is characterized by fewer externalizing behaviors, worse sustained attention, and better response inhibition function. The absence of abnormally high hyperactive/impulsive symptoms in ADHD-RI might be related to less impaired brain function in DMN, but potentially more impairment in the salience network.
Effectiveness of a mHealth intervention on hypertension control in a low-resource rural setting: A randomized clinical trial
Authors: Yuting Zhang , Xiaodong Tan , Qun Wang 
Published: March 2023

BackgroundDespite the increasing popularity of mHealth, little evidence indicates that they can improve health outcomes. Mobile health interventions (mHealth) have been shown as an attractive approach for health-care systems with limited resources. To determine whether mHealth would reduce blood pressure, promote weight loss, and improve hypertension compliance, self-efficacy and life quality in individuals with hypertension living in low-resource rural settings in Hubei, China.MethodsIn this parallel-group, randomized controlled trial, we recruited individuals from health-care centers, home visits, and community centers in low-resource rural settings in Hubei, China. Of 200 participants who were screened, 148 completed consent, met inclusion criteria, and were randomly assigned in a ratio of 1:1 to control or intervention. Intervention group participants were instructed to use the Monitoring Wearable Device and download a Smartphone Application, which includes reminder alerts, adherence reports, medical instruction and optional family support. Changes in the index of Cardiovascular health risk factors from baseline to end of follow-up. Secondary outcomes were change in hypertension compliance, self-efficacy and life quality at 12 weeks.ResultsParticipants (n = 134; 66 in the intervention group and 68 controls) had a mean age of 61.73 years, 61.94% were male. After 12 weeks, the mean (SD) systolic blood pressure decreased by 8.52 (19.73) mm Hg in the intervention group and by 1.25 (12.47) mm Hg in the control group (between-group difference, −7.265 mm Hg; 95% CI, −12.89 to −1.64 mm Hg; P = 0.012), While, there was no difference in the change in diastolic blood pressure between the two groups (between-group difference, −0.41 mm Hg; 95% CI, −3.56 to 2.74 mm Hg; P = 0.797). After 12 weeks of follow-up, the mean (SD) hypertension compliance increased by 7.35 (7.31) in the intervention group and by 3.01 (4.92) in the control group (between-group difference, 4.334; 95% CI, 2.21 to −6.46; P &lt; 0.01), the mean (SD) hypertension compliance increased by 12.89 (11.95) in the intervention group and by 5.43 (10.54) in the control group (between-group difference, 7.47; 95% CI, 3.62 to 11.31; P &lt; 0.01), the mean (SD) physical health increased by 12.21 (10.77) in the intervention group and by 1.54 (7.18) in the control group (between-group difference, 10.66; 95% CI, 7.54–13.78; P &lt; 0.01), the mean (SD) mental health increased by 13.17 (9.25) in the intervention group and by 2.55 (5.99) in the control group (between-group difference, 10.93; 95% CI, 7.74 to 14.12; P &lt; 0.01).ConclusionsAmong participants with uncontrolled hypertension, individuals randomized to use a monitoring wearable device with a smartphone application had a significant improvement in self-reported hypertension compliance, self-efficacy, life quality, weight loss and diastolic blood pressure, but no change in systolic blood pressure compared with controls.
The effect of carbon nanoparticles staining on lymph node tracking in colorectal cancer: A propensity score matching analysis
Authors: Liu Fei , Peng Dong , Liu Xiao-Yu , Liu Xu-Rui , Li Zi-Wei , Wei Zheng-Qiang , Wang Chun-Yi 
Published: March 2023

PurposeThe aim of this study was to evaluate the effect of carbon nanoparticles staining (CNS) on colorectal cancer (CRC) surgery, lymph node tracing and postoperative complications using propensity score matching (PSM).MethodPatients who were diagnosed with CRC and underwent surgery were retrospectively collected from a single clinical center from Jan 2011 to Dec 2021. Baseline characteristics, surgical information and postoperative information were compared between the CNS group and the non-CNS group. PSM was used to eliminate bias.ResultsA total of 6,886 patients were enrolled for retrospective analysis. There were 2,078 (30.2%) patients in the CNS group and 4,808 (69.8%) patients in the non-CNS group. After using 1: 1 ratio PSM to eliminate bias, there were 2,045 patients left in each group. Meanwhile, all of their baseline characteristics were well matched and there was no statistical significance between the two groups (P &gt; 0.05). In terms of surgical information and short-term outcomes, the CNS group had less intraoperative blood loss (P &lt; 0.01), shorter operation time (P &lt; 0.01), shorter postoperative hospital stay (P &lt; 0.01), less metastatic lymph nodes (P = 0.013), more total retrieved lymph nodes (P &lt; 0.01), more lymphatic fistula (P = 0.011) and less postoperative overall complications (P &lt; 0.01) than the non-CNS group before PSM. After PSM, the CNS group had less intraoperative blood loss (P = 0.004), shorter postoperative hospital stay (P &lt; 0.01) and more total retrieved lymph nodes (P &lt; 0.01) than the non-CNS group. No statistical difference was found in other outcomes (P &gt; 0.05).ConclusionPreoperative CNS could help the surgeons detect more lymph nodes, thus better determining the patient's N stage. Furthermore, it could reduce intraoperative blood loss and reduce the hospital stay.
Economic Benefits of Using Essential Oils in Food Stimulation Administrated to Bee Colonies
Authors: Pătruică Silvia , Lazăr Roxana Nicoleta , Buzamăt Genoveva , Boldea Marius 
Published: March 2023

Bees are the most important pollinators of agricultural plants. The decline of bee colonies is caused by a multitude of factors of which diseases, pesticides, and climate change seem to be the most important. Losses can be huge when several factors act together. In recent years, there has been a growing interest in the use of alternative sources such as medicinal plants in the form of extracts or essential oils. The purpose of our research was to evaluate the economic efficiency of the use of essential oils of basil (Ocimum basilicum), cinnamon (Cinnamomum veruum), clove (Syzgium aromaticum), juniper (Juniperus communis L.), oregano (Oreganum vulgare), mint (Mentha piperita), rosemary (Rosmarius officinalis), and thyme (Thymus vulgaris L.) in the additional feeding of bee colonies in the spring. Ninety colonies of Apis mellifera bees were used, which were additionally fed with sugar syrup and one of the essential oils. Our results showed strong positive correlations between the total number of germs in the gut of worker bees and the number of brood cells when using the essential oil of oregano (Oreganum vulgare) (R2 = 0.786) and thyme (Thymus vulgaris L.) (R2 = 0.729), and between the total number of germs and the yield of honey obtained at the first harvest in the case of the essential oil of basil (Ocimum basilicum) (R2 = 1), mint (Mentha piperita) (R2 = 0.718), oregano (Oreganum vulgare) (R2 = 0.621), and Thyme (Thymus vulgaris L) (R2 = 0.859). The best profit from the sale of honey was obtained in the case of the use of essential oils of mint, oregano, thyme, and basil, in a range of EUR 139.16–144.73/bee colony.
Desenvolvimento e avaliação de filmes orodispersíveis de melatonina obtidos através da prática magistral
Authors: Lamolha Marco Aurélio , De Senna Leila Alves , De Oliveira Diana Alves 
Published: March 2023

Os filmes orodispersíveis- FOD’s são formas farmacêuticas sólidas alternativas com grande potencial terapêutico e mercadológico, constituídas por polímeros hidrossolúveis que, administrados sobre a língua, ou na cavidade oral, se hidratam rapidamente na saliva liberando o fármaco, sem a necessidade de administração de água, ou mastigação. Foram recentemente introduzidos no mercado magistral nacional, através da adaptação do método solvent casting, com ou sem o emprego de um laminador. Com o objetivo de avaliar um dos processos de obtenção; o qual utiliza uma base pronta para uso, seringa de 1mL para distribuição da preparação sobre formas pré-moldadas, as características dos filmes obtidos, além de oferecer subsídios para auxiliar os farmacêuticos magistrais no desafio de manipular esta nova forma de veiculação de ativos, foram manipulados e avaliados filmes contendo 10mg de melatonina como fármaco modelo. Após 3horas de secagem, à temperatura de 50ºC, foram obtidos filmes translúcidos homogêneos, incolores, que foram avaliados quanto a espessura, peso, uniformidade de doses, % de umidade residual e tempos de desintegração. Os filmes apresentaram uniformidade em relação à espessura, peso e dosagem, umidade residual em torno de 8% e tempos de desintegração que atendem aos requisitos estabelecidos pelos códigos farmacêuticos oficiais europeu e americano para comprimidos orodispersíveis, que foram extrapolados para os filmes. O processo avaliado demonstrou ser de fácil execução, bastante reprodutível e sem a necessidade de um grande tempo de aprendizado e experiência por parte do manipulador.
Metaphorical Perceptions of High School Students about the Literature Course
Authors: Savaşkan Vafa 
Published: March 2023

We aimed to determine high school student perceptions about the literature course based on metaphors. We conducted the study with the phenomenology model, a qualitative research method. The study group included 137 students (57 female and 80 male students) attending ninth to twelfth grades at four public high schools in Sinop province in Turkey. Fourty-six students were 9th graders, 40 were 10th graders, 32 were 11th graders, and 19 were 12th graders. The study data were collected with the phrase “Literature course…. Is like…, because,…” completed by the participants. Descriptive analysis was conducted on the study data. Data analysis included elimination and selection, naming, category development, validity and reliability phases. The study findings demonstrated that 137 high school students came up with 126 metaphors. Fifty-four metaphors were produced by female students and 72 by male students. These metaphors included life (f12), poetry (f4), death (f2), art (f3), book (f7), dictionary (f4), sun (f3). Twenty-four metaphors were produced by the 9th graders, 30 by the 10th graders, 27 by the 11th graders, and 15 by the 12th graders. In conclusion, although certain metaphors were negative, the majority of the metaphors were positive.
Analysis of The Accuracy of Temperature Sensors at The Calibrator Incubator Laboratory are equipped with data storage base on Internet of Thing
Authors: Prastyadi Candra , Irianto Bambang Guruh , Ariswati Her Gumiwang , Titisari Dyah , Nyatte Steyve , Misra Shubhrojit 
Published: March 2023

A laboratory incubator is a tool used to incubate or incubate a breed. Incubators provide optimum temperature conditions for microorganisms to grow. The incubator has a temperature regulator so that the temperature can be adjusted according to the breed to be incarnated. Incubators utilize hot-dry like ovens. The purpose of this study is to conduct testing and analyze the accuracy of thermocouple sensors with incubator media in laboratory incubator calibrator tools. The contribution of the research is to know the level of accuracy of the sustainable sensor for sensing the temperature in the lab incubator. The main Design consists of 8 MAX 6675 Standards, 8 Thermocouple type K, Arduino Mega, and SD Card Standards. The temperature not in the incubator device is measured by a Type K thermocouple sensor. Thermocouple sensor numbers 8 channels that measure the temperature at each incubator camber point. The temperature will be stored on the SD card to analyze the data and the data can be processed into the form of a graphic. Benchmarking is done using a data logger temperature tool. This is done to make the Design results are under the standards of the Standard. After comparing with the Standard get the largest error value is 3.98%, at channel T6 temperature 35 °C with ordinary incubator media and the smallest error in ordinary incubator media point T6 temperature 37 ° C which is 0.06 % and in fan incubator temperature 35 C has the largest error which is 2.98 % and the smallest error 0.86%. The conclusion of this study is that the design can work well in measuring the temperature of the incubator, as well as the system for storing readings using the SD card Design and sending data using the internet network can work well.
INNOVATIVE SOLUTIONS IN MODERN HEAT SUPPLY: ASPECTS OF IMPLEMENTATION
Authors: Верстин Николай Александрович , Дигилина Ольга Борисовна 
Published: March 2023

В статье рассматриваются перспективы использования инновационных решений для развития теплоснабжения в странах постсоветсткого пространства, которые формировались в условиях действия сходных технических и организационных факторов. Представлены результаты обзора инноваций из открытых источников, а также актуализи-рованные цели использования инновационных решений в теплоснабжающих организациях.The article discusses the prospects of using innovative solutions for the development of heat supply in the post-Soviet countries, which were formed under the influence of similar technical and organizational factors. The results of the review of innovations from open sources are presented, as well as updated goals for the use of innovative solutions in heat supply organizations.
Developing Contract Soldiers’ Reading Culture: Pedagogical Aspects
Authors: Щелкунов Д.В. 
Published: March 2023

В статье решается научная задача — оценка значения читательской культуры в становлении профессионализма воинов-контрактников и определения педагогических подходов к ее успешному формированию. Феномен читательской культуры рассматривается как универсальное средство духовно-нравственного развития личности военнослужащего в контексте воспитательной деятельности. На основе анализа содержания понятия читательской культуры в сравнении с понятием культуры чтения обосновывается целесообразность применения именно понятия читательской культуры в контексте формирования профессионализма военнослужащего. В контексте содержания профессионализма военнослужащих по контракту читательская культура трактуется как интегральная характеристика личности военнослужащего-контрактника. Ее смысловым ядром выступает система ценностей и ценностных ориентаций, определяющая методы и формы освоения письменных текстов посредством чтения и обеспечивающая совершенствование воинского профессионализма. Предлагаемая содержательная интерпретация понятия читательской культуры выступает основой для педагогического моделирования процесса ее формирования. Ценностно-смысловое содержание читательской культуры рассматривается в статье на примере приобщения к военно-историческим традициям посредством чтения. Исходя из выработанной автором на основе теоретического анализа научной литературы дефиниции понятия «читательская культура военнослужащих по контракту» определяются основные педагогические подходы к развитию читательской культуры воинов-контрактников.
The article focuses on the importance of improving contract soldiers’ reading culture, which is treated as a cultural environment and a universal strategy of military personnel’s moral development. The article maintains that reading culture should be viewed as a component of military personnel’s professional development. When viewed through the prism of military personnel’s professionalism, reading culture can be defined as an integral characteristic of a contract soldier. Being the pivotal element of reading culture, values and moral guidelines ensure the development of military personnel’s professionalism. It is important that reading culture should be properly defined, for its definition predetermines the way it is developed. The article underlines that reading culture development presupposes soaking up military traditions of the previous centuries through reading. The author analyzes various scholarly sources to define contract soldiers’ reading culture and to outline basic approaches to its development.
Disentangling Modified Gravity and Massive Neutrinos with Intrinsic Shape Alignments of Massive Halos
Authors: Lee Jounghun , Ryu Suho , Baldi Marco 
Published: March 2023

Abstract
We present two new diagnostics based on the intrinsic shape alignments of group/cluster size dark matter halos to disentangle the effect of f(R) gravity from that of massive neutrinos. Using snapshot data from a series of the DUSTGRAIN-pathfinder N-body simulations for a Planck ΛCDM cosmology and three f(R) gravity models with massive neutrinos (ν), we first determine the probability density functions of the alignment angles between the shape orientations of massive halos and the minor principal axes of the local tidal fields. The numerically obtained results turn out to agree very well with the analytic formula derived under the assumption that the anisotropic merging along the cosmic web induces the halo shape alignments. The four cosmologies, which several standard diagnostics failed to discriminate, are found to yield significantly different best-fit values of the single parameter that characterizes their analytic formulae. We also numerically determine the spatial cross-correlations between the shape orientations of neighbor group/cluster halos, and find them to be in good agreements with a fitting formula characterized by two parameters, whose best-fit values are found to differ substantially among the four models. We also discuss the limitations and caveats of these new diagnostics that must be overcome for their application to real observational data.
Relativistic Hybrid Stars with Sequential First-order Phase Transitions in Light of Multimessenger Constraints
Authors: Li Jia Jie , Sedrakian Armen , Alford Mark 
Published: March 2023

Abstract
In this work, we consider the properties of compact stars in which quark matter has low- and high-density phases that are separated by a first-order phase transition. Thus, unlike the commonly considered case of a single phase transition from hadronic to quark matter, our models of hybrid stars contain sequential phase transitions from hadronic matter to low- and then to high-density quark matter phases. We extend our previous study of the parameter space of hybrid stars with a single phase transition to those with sequential phase transitions, taking into account the constraints on the mass and radius of neutron stars from the NICER experiment, the experimental inferences of the neutron skin thickness of the lead nucleus by the PREX-II experiment, and constraints on the tidal deformability from the gravitational-wave event GW170817. We determine the range of the masses for which both twin and triplet configurations, i.e., identical-mass stars with two and three different values of radii, arise.