bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying potential biomarkers in acute myocardial infarction based on neutrophil extracellular traps associated genes
Authors: Cao Ye , Wang Qing-Feng , Li Bo , Zou Yuan-Lin , Zhang Jian-Cheng 
Published: May 2023

Abstract
Background Acute myocardial infarction (AMI) represents one of the major critical cardiovascular disorders due to its high mortality and morbidity. Neutrophil extracellular traps(NETs) are essential throughout the thrombotic process of AMI. However, genes associated with NETs in AMI have not been fully described.Methods NETs-associated gene candidates were identified by literature review. AMI-associated datasets(GSE66360) were retrieved from Gene Expression Omnibus (GEO) database. Differentially expressed NETs-associated genes were subjected to Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis. The marker genes were subsequently selected by the least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE) algorithms and calculated based on the receiver operating characteristic (ROC) curve. To further probe the potential features of these marker genes, single-gene gene set enrichment analysis (GSEA) was performed. To further discuss immune microenvironment modulations, immune infiltration analysis was performed by CIBERSORT algorithms. Accordingly, an mRNA-miRNA-lncRNA network was constructed. Finally, gene expression levels of these marker gene were verified according to an external dataset (GSE66145).Results Forty-five differentially expressed NETs-associated genes were screened out from the GSE66360 dataset, which was closely linked to myeloid leukocyte activation and inflammatory response. FCAR, LILRB2, PDE4B, S100A12, DNASE1, IL1B, IL6, MMP9, and TLR2 were identified as marker genes. The AUC of marker genes was higher than 0.6 and the AUC of the marker genes-based logistic regression model was 0.981. Functional enrichment analysis results suggested that these marker genes might exert consequential effects in AMI through regulating immune responses. CIBERSORT analysis further revealed that the immune microenvironment alterations may be associated with TLR2, S100A12, LILRB2, IL1B, and FCAR. In addition, the ceRNA network demonstrated a complex regulatory interaction.Conclusion Here we identified and validated 9 NETs-associated genes (FCAR, LILRB2, PDE4B, S100A12, DNASE1, IL1B, IL6, MMP9, and TLR2) as novel biomarkers in AMI pathogenesis. These genes may be involved in the onset and development of AMI through NETs formation. Collectively, our findings have provided potential targets for the diagnosis and treatment of AMI.
Modeling the Structural Relationships Between Trauma Experience with Substance Use Tendency, Depression Symptoms, and Suicidal Thoughts in Individuals with Earthquake Trauma Experience: The Mediatory Role of Peritraumatic Dissociation and Experiential Avoidance
Authors: Ahmadi Farshad , Goodarzi Mohammad Ali , Taghavi Mohammad Reza , Imani Mahdi 
Published: May 2023

Abstract
Background: A significant portion of studies are mainly focused on the formation of post-traumatic stress disorder (PTSD) after earthquake trauma. This is while earthquakes are associated with a wide range­ of ­psychological reactions­. In addition, other ­factors play­ a critical role in the formation of a diverse range of mental problems - personal characteristics, the severity of exposure to earthquake, and post-earthquake psychological distress (e.g., dissociation and avoidance). Therefore, This study investigates the relationship between earthquake intensity experience with substance use tendency, depression, and suicidal thoughts with the mediating role of peritraumatic dissociation and experiential avoidance.
Methods: The participants consist of 323 individuals who had experienced an earthquake disaster four years ago. A set of valid and reliable questionnaires­ was administered to the participants. The gathered data were analyzed‌ using structural equation modeling in ‌SPSS Ver. 24 and LISREL Ver. 24.
Results: The results showed that the intensity of the trauma experience is directly and significantly associated with depression symptoms, peritraumatic dissociation, and experiential avoidance. The experience of trauma had a significant­ indirect effect on the tendency to use substance through experimental­ avoidance. This is while the severity of the trauma experience did not directly correlate with substance use and suicidal thoughts. In addition, peritraumatic dissociation did not mediate the relationship between the severity of trauma experience with substance use, depression, and suicidal thoughts.
Conclusions­: It seems ­that both the experience (severity) of earthquake trauma and some reactions by individuals after a trauma experience can contribute to the prediction of some psychological consequences. These findings indicate the significance of experiential avoidance­ in predicting substance use tendencies. Nonetheless, to draw a clearer picture of­ peritraumatic dissociation, future research should ­focus on­ measuring ­this component more precisely and the inclusion of constructs­ that are naturally dissociative­.
Impact of malnutrition on cancer recurrence, colorectal cancer-specific death, and non-colorectal cancer- related death in patients with colorectal cancer who underwent curative surgery
Authors: Nakamura Yuya , Kawase Makoto , Kawabata Yusuke , Kanto Satoshi , Yamaura Tadayoshi , Kinjo Yousuke , Ogo Yasumasa , Kuroda Nobukazu 
Published: May 2023

Abstract
Purpose The impact of malnutrition on colorectal cancer (CRC) recurrence and cause of death remains poorly understood. The present study aimed to examine how malnutrition, as reflected by the Geriatric Nutritional Risk Index (GNRI), is associated with CRC recurrence and cause of death.Methods Subjects were 601 consecutive stage I-III CRC patients who underwent curative surgery between 2011 and 2017. We divided patients into two groups using GNRI 98 as the cut-off and evaluated the relationship of GNRI with overall survival (OS) and recurrence-free survival (RFS). Competing risk analysis was performed to determine prognostic factors of non-CRC-related death. Hazard functions were then used to examine changes in the risk of recurrence and death in each GNRI group over time.Results The low GNRI group predicted significantly worse OS and RFS in multivariable analyses. Moreover, Fine-Gray model regression revealed that the low GNRI group was a significant predictor of non-CRC-related death. Hazard rate of recurrence and death in the low GNRI was approximately double that observed in the high GNRI group over 5 years; meanwhile, the hazard rate for recurrence in the low GNRI group was similar to that in the high GNRI group after 3 years. Meanwhile, the low GNRI group had a higher risk of non-CRC-related death over the course of 5 years.Conclusions While preoperative malnutrition has a strong prognostic impact, its effect over time differs when endpoints are considered separately. These findings provide valuable information regarding healthcare prioritization during CRC survivorship.
Study on the landslide mechanism of the loess slopes induced by rainfall
Authors: Liu Wei , BAI Ruiqiang , SUN Xinran , Liu Yuhang , SU Xing 
Published: May 2023

Abstract
To reveal the landslide mechanism of the loess slope induced by rainfall, we carried out a series of tests for loess under different permeability durations, and studied the influences of rainfall on the permeability coefficient, the soil water characteristic curve, the components, the microstructure, and the shear strength of the loess. Test results show that the total dissolved solids, the sand particles, and the clay particles will decrease, but the silt particles will increase with the permeability duration, it changes the soil original structure and influences the soil water characteristic curve of the loess. And more than 68% permeability coefficients will loss after permeability test. The infiltrated rainwater will increase the effective saturation of the soil, and the shear strength of the soil will decrease with the increasing of effective saturation. The shear strength further decreases with the infiltration time (or rainfall duration). The landslide will initial once the shear strength reduces to the geostatic stress of the soil slope. The shear strength loss of the soils induced by rainfall should be considered during slope stability analysis. This study can help to understand the initiation of loess landslide induced by rainfall.
Failure to rescue as a quality metric in congenital heart surgeries in a high- complexity service provider institution located in a middle-income country
Authors: Cruz Gustavo , Pedroza Santiago , Vélez Juan F. , Largo Jessica , Tejada Juan F. , Mejía Jorge Humberto 
Published: May 2023

Abstract

Background Failure to rescue has been an effective quality metric in congenital heart surgery. Conversely, morbidity and mortality depend greatly on non-modifiable individual factors and have a weak correlation with better-quality performance. We aim to measure the complications, mortality, and risk factors in pediatric patients undergoing congenital heart surgery in a high-complexity institution located in a low-income country and compare it with other institutions that have conducted a similar study.Methods A retrospective observational study was conducted in a high-complexity service provider institution, in Cali, Colombia. All pediatric patients undergoing any congenital heart surgery between 2019 and 2022 were included. The main outcomes evaluated in the study were complication, mortality, and failure to rescue rate. Univariate and multivariate logistic regression analysis was performed with mortality as the outcome variable.Results We evaluated 308 congenital heart surgeries. Regarding the outcomes, 201 (65%) complications occurred, 23 (7.5%) patients died, and the FTR of the entire cohort was 11.4%. The presence of a postoperative complication (OR 14.88, CI 3.06-268.37, p = 0.009), age (OR 0.79, CI 0.57–0.96, p = 0.068), and urgent/emergent surgery (OR 8.14, CI 2.97–28.66, p < 0.001) were the most significant variables in predicting mortality.Conclusions Failure to rescue is an effective and comparable quality measure in healthcare institutions and is the major contributor to postoperative mortality in congenital heart surgeries. Despite our higher mortality and complication rate, we obtained a comparable failure to rescue rate to high-income countries' health institutions.
Selfing revealed positive values than backcrossing for yield and yield enhancing traits among tomato segregating populations generated from Solanum lycopersicum × S. pimpinellifolium crosses under tropical humid climate
Authors: Ene Chikezie Onuora , Abtew Wosene Gebreselassie , Oselebe Happiness Ogba , Chukwudi Uchechukwu Paschal , Okechukwu Emeka Chibuzor , Ozi Friday Ugadu , Ene Chibueze Kelechi , Menamo Temesgen Matiwos , Atugwu Agatha Ifeoma 
Published: May 2023

Abstract

The objectives of this study were to assess phenotypic variability among F3 and BC1F2 tomato populations, and apply genotype by yield*trait (GYT) biplots for population and line selection based on multiple traits. Four diverse cultivated parents (‘CLN2498D’ and ‘CLN2417H’ from Ethiopia; ‘UC Dan INDIA’ and ‘Tima’ from Nigeria), and wild parent ‘LA2093’ were used to generate 276 potential breeding lines. The lines were categorized into eight populations (‘Pop_1_W/H1’, ‘Pop_2_W/H2’, ‘Pop_3_W/D1’, ‘Pop_4_W/D2’, ‘Pop_5_W/T1’, ‘Pop_6_W/T2’, ‘Pop_7_W/U1’, and ‘Pop_8_W/U2’), and evaluated twice in the field using 19 × 15 alpha-lattice design with two replicates. Significant differences were observed among lines and populations for all yield enhancing traits. ‘Pop_1_W/H1’, ‘pop_4_W/D2’ and ‘pop_6_W/T2’ expressed the highest genetic divergence for plant height, number of leaves, total flower and fruit number, and fruit weight. GYT biplots revealed that all yield*trait interactions had a positive correlation with each other. F3 populations, ‘Pop_5_W/T1’ and ‘pop_1_W/H1’ exhibited the best performance for majority of the yield*trait combinations. Hierarchical clustering on principal components (HCPC) revealed overlapping lines (70.58% of Cluster D lines) and (54.05% of Cluster U lines) from the two F3 populations. In BC1F2 population, 32.35% of the 34 original lines of Cluster D and 48.48% of Cluster T lines overlapped between Clusters D and T, while 18.18% of Cluster T lines and 8.82% of Cluster H lines were transgressive between Clusters T and H. Transgressive segregants ‘0210U1’, ‘0211U1’, and ‘0171T1’ of selfed population using multivariate analysis were believed to represent potential sources of novel genetic variation for future tomato breeding.
Enhancing C. reinhardtii growth and metabolite biosynthesis using organic dyes as spectral convertors
Authors: Ramanna Luveshan , Nasr Mahmoud , Rawat Ismail , Bux Faizal 
Published: May 2023

Abstract
The commercial success of microalgal systems has to a large extent been restricted due to limited light availability and utilisation. Algae utilize the blue and red spectral regions, large amounts of the incoming light energy fall outside this region and remain unused. The spectral conversion of unusable to usable wavelengths will increase the amount of radiation available for photosynthesis. This study was conducted to determine the optimum organic dye among Lumogen Red (LR), Rhodamine 8G (R8G), and Lumogen Yellow (LY) as spectral converters, providing the best light utilisation efficiency in an algal system. The microalga Chlamydomonas reinhardtii was cultivated in double-jacketed cylindrical photo-bioreactors (PBRs) stimulated using 30% UV-a and 5% UV-b fluorescent tubes. Carbohydrate and lipid contents were produced early in the growth phase, whereas biomass and protein content increased after day 4. The LR dye maintained better photo-stability compared to R8G and LY, which displayed a slight wavelength shift and photo-degradation, respectively. The improved biomass production in the LR-assisted PBR was illustrated by increased Y(II), with decreasing Y(NPQ), total chlorophylls, and carotenoids. Pigment biosynthesis was initially increased and then reduced to compensate for the increased irradiation as a means of photoprotection. Gene expression studies showed that algae grown under LR had a 1.6- and 2.9-fold up-regulation of the RuBisCo gene. The applicability of the dye-based algal system was linked to enhanced growth, lipid, protein, carbohydrate, and pigment productions. Moreover, the environmental, economic, and social aspects of improving microalgal cultivation using dye-based wavelength conversion systems were interlinked to the sustainable development goals.
"There is no one who helps you with it." Experiences of people with long COVID regarding medical care, therapeutic measures, and barriers in the German healthcare system: results of a qualitative study with four focus groups
Authors: Schmachtenberg Tim , Königs Gloria , Dragaqina Anita , Roder Sascha , Müller Frank , Müllenmeister Christina , Schröder Dominik , Dopfer-Jablonka Alexandra , Vieth Katharina , El-Sayed Iman 
Published: May 2023

Abstract
Background
Many people experience long-term symptoms such as fatigue, cognitive problems, or shortness of breath after an acute infection with COVID-19. This emerging syndrome, known as long COVID, is new and complex in many aspects. This study aims to collect the experiences of people with long COVID with ambulatory healthcare structures.
Methods
Four focus groups were conducted with a total of 23 adults with long COVID in June and July 2022. These discussions were audio-recorded, subsequently transcribed, and analyzed using the qualitative content analysis of Mayring and Kuckartz.
Results
Fourteen out of 19 participants who had a primary care encounter regarding their long COVID symptoms did not perceive it as helpful. Many respondents reported that their general practitioners did not take their long COVID symptoms seriously and did not refer them to specialists or made therapeutic recommendations. However, some participants reported that they were prescribed non-pharmaceutical therapies (e.g., group meetings supported by psychotherapists, occupational therapy, etc.) that improved their condition. 14 of 23 respondentsperceived care barriers such as providers' lack of awareness of long COVID, poor access to specialists, a lack of specialized care (e.g., long COVID clinics), or high bureaucratic hurdles for specific healthcare services. To improve medical care, participants suggested campaigns to raise awareness of long COVID among healthcare providers and the general population, increase research and government investments regarding the development of treatment structures for long COVID, expanding existing therapeutic services, and establishing one-stop shops for integrated specialist healthcare for people with long COVID.
Conclusions
Several implications for healthcare professionals and policymakers can be derived from this study: (1) general practitioners should take the symptoms of long COVID seriously, assume a care coordinating role, make referrals, and establish contact with long COVID clinics; (2) care planners should focus on developing interprofessional evidence-based care and treatment approaches for long COVID; (3) existing care structures such as long COVID outpatient clinics should be expanded.
Trial registration: The study is registered in the German register for clinical trials (DRKS00026007).
The Influence of Academic Stress on College Students' Learning Motivation: The Mediating Role of Academic Environment Satisfaction and the Moderating Role of Learning Adaptability
Authors: Liu Chen , Tang Jiayi , Jiang Zhou , Zhan Xingya , Shen Junjie , Wang Mengying , Shen Chao , Liu Xin 
Published: May 2023

Abstract
The relationships between academic stress, learning motivation, academic environment satisfaction, and learning adaptability were examined in a group of undergraduate students in Jiangsu Province, China. The survey was completed by 1666 students, with 1607 students whose questionnaire was complete and valid. College students' learning motivation was positively correlated with their academic stress (r = 0.218, P < 0.01), academic environment satisfaction (r = 0.028, P < 0.01), and learning adaptability (r = 0.108, P < 0.01). Academic environment satisfaction mediated the relationship between academic stress and learning motivation. Learning adaptability moderated the first half of the path of "academic stress → academic environment satisfaction → learning motivation". In particular, academic stress was a stronger predictor of academic environment satisfaction in the low level of learning adaptability. Thus, in order to enhance learning motivation, we can achieve students' satisfaction by changing the academic environment. In addition, adjusting learning adaptability is also an important way to improve learning motivation through academic environment satisfaction.
Propofol reversibly attenuates short-range microstate ordering and 20 Hz microstate oscillations
Authors: Hermann Gesine , Tödt Inken , Tagliazucchi Enzo , Todtenhaupt Inga Karin , Laufs Helmut , von Wegner Frederic 
Published: May 2023

Abstract
Microstate sequences summarize the changing voltage patterns measured by electroencephalography (EEG), using a clustering approach to reduce the high dimensionality of the underlying data. A common approach is to restrict the pattern matching step to local maxima of the global field power (GFP) and to interpolate the microstate fit in between. In this study, we investigate how the anesthetic propofol affects microstate sequence periodicity and predictability, and how these metrics are changed by interpolation. We performed two frequency analyses on microstate sequences, one based on time-lagged mutual information, the other based on Fourier transform methodology, and quantified the effects of interpolation. Resting-state microstate sequences had a 20 Hz frequency peak related to dominant 10 Hz (alpha) rhythms, and the Fourier approach demonstrated that all five microstate classes followed this frequency. The 20 Hz periodicity was reversibly attenuated under moderate propofol sedation, as shown by mutual information and Fourier analysis. Characteristic microstate frequencies could only be observed in non-interpolated microstate sequences and were masked by smoothing effects of interpolation. Information-theoretic analysis revealed faster microstate dynamics and larger entropy rates under propofol, whereas Shannon entropy did not change significantly. In moderate sedation, active information storage decreased for non-interpolated sequences. Signatures of non-equilibrium dynamics were observed in non-interpolated sequences only and decreased in moderate sedation. All changes occurred while subjects were able to perform an auditory perception task. In summary, we show that low-dose propofol reversibly increases the randomness of microstate sequences and attenuates microstate oscillations without correlation to cognitive task performance. Microstate dynamics between GFP peaks reflect physiological processes that are not accessible in interpolated sequences.
Effect of microalgae diets on growth, proximate and fatty acid composition of black mussel (Mytilus galloprovincialis)
Authors: Pınarbaşı Zeynep Sena , Sayar Merve , Türk Sare Sena , Tınkır Merve , Eryalçın Kamil Mert 
Published: May 2023

Abstract
The aim of this study was to evalute the growth, proximate and fatty acid composition of Black mussel (Mytilus galloprovincialis), which are common bivalve species that important for marine aquaculture in last two decades. Nutritional value and growth dynamics are essential for larval and juvenile stages of cultured animals. In this study, four fresh microalgae (Pavlova lutheri, Nannochloropsis oculata, Diacronema vlkianum and Rhinomonas reticulata) have been studied. Mussels collected from the Marmara Sea were adapted to experimental conditions for 2 days and fed for 3 weeks. Shell height, lenght and witdh of mussels were measured. The height and length of mussels fed with Nanochloropsis oculata measured statistically higher than other experimental groups. Shell widths did not show any significant differences among groups. The proximate analysis revealed that Nanochloropsis oculata has the highest protein content and Pavlova lutheri has the highest lipid content. Fatty acid compositions were analyzed at the end of the experiment. Diacronema vlkianum diet revealed the highest docosahexaenoic acid (DHA) content. Black mussel fed Pavlova lutheri diet showed the highest eicosapentaenoic acid (EPA) and polyunsaturated fatty acid (PUFA) content. Arachidonic acid (ARA) ratios appeared to be similar among mussels fed with all groups.