bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beyond High-Income Countries: Low Numeracy Is Associated with Older Adult Age around the World
Authors: Bruine de Bruin Wändi , Ulqinaku Aulona , Llopis Jimena , Ravà Matteo Santangelo 
Published: May 2023

Background Numeracy, or the ability to understand and use numbers, has been associated with obtaining better health and financial outcomes. Studies in high-income countries suggest that low numeracy is associated with older age—perhaps especially among individuals with lower education. Here, we examined whether findings generalize to the rest of the world. Methods Gallup surveyed >150,000 participants for the 2019 Lloyd’s Register Foundation World Risk Poll, from 21 low-income, 34 lower-middle income, 42 upper-middle income, and 43 high-income countries. Low numeracy was operationalized as failing to correctly answer, “Is 10% bigger than 1 out of 10, smaller than 1 out of 10, or the same as 1 out of 10?” Results Regressions controlling for participants’ education, income, and other characteristics found that, worldwide, low numeracy was associated with older age, lower education, and their interaction. Findings held in each country-income category, although low numeracy was more common in low-income countries than in high-income countries. Limitations Age differences may reflect cohort effects and life span–developmental changes. Discussion Low numeracy is more common among people who are older and less educated. We discuss the need for education and interventions outside of the classroom. Highlights We analyzed a global survey conducted in 21 low-income, 34 lower-middle income, 42 upper-middle income, and 43 high-income countries. Low numeracy was associated with older adult age, even after accounting for age differences in education. Low numeracy was more common in older people with lower education.
Prediction and outcomes of cerebral vasospasm in ischemic stroke patients receiving anterior circulation endovascular stroke treatment
Authors: Jesser Jessica , Awounvo Sinclair , Vey Johannes A , Vollherbst Dominik F , Hilgenfeld Tim , Chen Min , Nguyen Thanh N , Schönenberger Silvia , Bendszus Martin , Möhlenbruch Markus A , Weyland Charlotte S 
Published: May 2023

Background and purpose: Vasospasm is a common complication of endovascular therapy (EVT). There is a lack of understanding of risk factors for periprocedural vasospasm. Here, we aimed to identify factors associated with vasospasm in patients with acute ischemic stroke who undergo EVT. Methods: We conducted a retrospective single-center analysis of patients receiving EVT for anterior circulation vessel occlusion between January 2015 and December 2021. Patients were excluded if they showed signs of intracranial atherosclerotic disease (ICAD) or if they underwent intra-arterial thrombolysis. Study groups were defined as patients developing vasospasm during EVT (V+) and patients who did not (V−). The study groups were compared in univariable analysis. Multivariable regression models were developed to predict the patient’s risk for developing vasospasm based on pre-identified potential prognostic factors. The secondary endpoint was clinical outcome defined as the modified Rankin Scale (mRS) difference between pre-stroke mRS and discharge mRS (delta mRS) and likelihood of successful reperfusion (TICI 2b/3). Results: In total, 132/1768 patients (7.5%) developed vasospasm during EVT. Vasospasm was more likely to occur in EVT with multiple thrombectomy attempts and after several stent retriever maneuvers. Factors associated with developing vasospasm were younger age (OR = 0.967, 95% CI = 0.96–0.98) and lower pre-stroke mRS (OR = 0.759, 95% CI = 0.63–0.91). The prediction model incorporating patient age, pre-stroke mRS, stent retriever thrombectomy attempts, and total attempts as prognostic factors was found to predict vasospasm with good accuracy (AUC = 0.714, 95% CI = 0.709–0.720). V+ patients showed higher median (IQR) delta mRS (2 (1–4) vs 2 (1–3); p = 0.014). There was no difference in successful reperfusion (TICI 2b-3) between those with or without vasospasm. Conclusion: Vasospasm was a common complication in EVT affecting younger and previously healthy patients. Presence of vasospasm did not reduce the likelihood of successful reperfusion. As independent predictors, patient age, pre-stroke mRS, thrombectomy maneuvers, and stent retriever attempts predict the occurrence of vasospasm during EVT with good accuracy.
Coverage Gaps and Inconsistencies: The Landscape of Insurance Coverage for Orthognathic Surgery in the United States
Authors: Akhavan Arya Andre , Ibelli Taylor , Benaroch David , Rodriguez Nina , Liu Helen , Ha Michael , Taub Peter James 
Published: May 2023

Background: Despite established indications for orthognathic surgery, insurance coverage is not guaranteed and obtaining coverage is often onerous. There is a paucity of literature regarding insurance coverage within the United States for orthognathic surgery. Methods: The top 50 health insurers in the US, and the top 3 per state, were selected using the National Association of Insurance Commissioners (NAIC) 2020 Market Share Report. Coverage policies were obtained online, or by phone when unavailable. Indications and criteria were compared to the American Association of Oral and Maxillofacial Surgeons (AAOMF) “Criteria for Orthognathic Surgery” recommendations. Results: Of the 65 insurance providers reviewed, 33 offered coverage with clear criteria, 24 offered coverage without listed criteria, and 8 explicitly excluded all orthognathic surgery. The 33 insurers with clear criteria were further reviewed. For malocclusion, 20 insurers used at least 75% of AAOMF measurements, while 4 used stricter criteria. Eleven insurers covered surgery for any congenital disorder; of the remaining, only 13 explicitly covered cleft-associated abnormalities. For the 25 with specified speech anomaly criteria, 21 offered coverage, and 4 excluded coverage. Sleep apnea coverage criteria were inconsistent, and temporomandibular joint disorder (TJMD) coverage was evenly split. No insurer covered orthognathic surgery for esthetic or psychological reasons. Among 66 identified unique criteria, only 6 significantly differed by US Census Region, with coverage for sleep apnea more prevalent in the South District. Conclusions: Access to insurer policies remains difficult. Insurers have inconsistent covered indications, strictness of coverage criteria, and exclusions, which frequently do not match standards of care. The authors present a list of the most commonly used criteria as a resource for insurance submissions, and encourage surgeons to advocate at the insurer and legislative levels for consistent coverage indications and criteria across insurance providers.
Tennantite-(Ni), Cu6(Cu4Ni2)As4S13, from Luobusa ophiolite, Tibet, China: a new Ni member of the tetrahedrite group
Authors: Wang Yanjuan , Chen Rujun , Gu Xiangping , Hou Zengqian , Nestola Fabrizio , Yang Zhusen , Fan Guang , Dong Guochen , Ye Lijuan , Qu Kai 
Published: May 2023

AbstractThe new mineral tennantite-(Ni), Cu6(Cu4Ni2)As4S13, has been discovered from the Luobusa Chromitite, Tibet, southwestern China. Tennantite-(Ni) occurs as anhedral grains ranging from 2 to 20 μm in size. In reflected light microscopy, tennantite-(Ni) is isotropic and appears yellow-greenish grey. Reflectance data for Commission on Ore Mineralogy wavelengths in air for tennantite-(Ni) are: 31.0 (470 nm), 29.6 (546 nm), 29.6 (589 nm) and 29.3 (650 nm). Electron microprobe analysis for holotype material gave the empirical formula (on basis of total cations = 16 apfu): M(2)Cu6M(1)[Cu4.00(Ni0.97Cu0.53Fe0.50)Σ2.00]Σ6.00X(3)(As2.94Sb1.06)Σ4S12.77. Tennantite-(Ni) is cubic, with space group I$\bar{ 4}$3m (#217), a =10.2957(9) Å, V = 1091.4(3) Å3 and Z = 2. By using single-crystal X-ray diffraction, the crystal structure has been determined and refined to a final R1 = 0.0423 on the basis of 163 independent reflections [Fo > 4σ (Fo)]. The calculated seven strongest powder X-ray diffraction lines [d in Å (I) (hkl)] are: 2.972 (100) (222), 1.820 (83) (440), 2.574 (28) (400), 1.552 (18) (622), 3.640 (10) (220), 1.880 (10) (521) and 1.287 (7) (800). Tennantite-(Ni) is isostructural with other tetrahedrite-group minerals, and nickel is hosted at the tetrahedrally coordinated M(1) site, along with Cu and minor Fe. The mineral and its name have been approved by the Commission on New Minerals, Nomenclature and Classification of the International Mineralogical Association (IMA2021-018).
Interim PET-guided treatment for early-stage NLPHL: a subgroup analysis of the randomized GHSG HD16 and HD17 studies
Authors: Eichenauer Dennis A. , Bühnen Ina , Baues Christian , Kobe Carsten , Kaul Helen , Greil Richard , Moccia Alden , Zijlstra Joseé M. , Hertenstein Bernd , Topp Max S. , Just Marianne , von Tresckow Bastian , Eich Hans-Theodor , Fuchs Michael , Dietlein Markus , Hartmann Sylvia , Engert Andreas , Borchmann Peter 
Published: May 2023

Abstract
The optimal first-line treatment for nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) diagnosed in early stages is largely undefined. We, therefore, analyzed 100 NLPHL patients treated in the randomized HD16 (early-stage favorable; n = 85) and HD17 (early-stage unfavorable; n = 15) studies. These studies investigated the omission of consolidation radiotherapy (RT) in patients with a negative interim positron emission tomography (iPET) (ie, Deauville score &lt;3) after chemotherapy (HD16: 2× doxorubicin, bleomycin, vinblastine, and dacarbazine [ABVD]; HD17: 2× escalated bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone [BEACOPP] plus 2× ABVD). Patients with NLPHL treated in the HD16 and HD17 studies had 5-year progression-free survival (PFS) rates of 90.3% and 92.9%, respectively. Thus, the 5-year PFS did not differ significantly from that of patients with classical Hodgkin lymphoma treated within the same studies (HD16: P = .88; HD17: P = .50). Patients with early-stage favorable NLPHL who had a negative iPET after 2× ABVD and did not undergo consolidation RT tended to have a worse 5-year PFS than patients with a negative iPET who received consolidation RT (83% vs 100%; P = .05). There were 10 cases of NLPHL recurrence. However, no NLPHL patient died during follow-up. Hence, the 5-year overall survival rate was 100%. Taken together, contemporary Hodgkin lymphoma-directed treatment approaches result in excellent outcomes for patients with newly diagnosed early-stage NLPHL and, thus, represent valid treatment options. In early-stage favorable NLPHL, consolidation RT appears necessary after 2× ABVD to achieve the optimal disease control irrespective of the iPET result.
Platelets regulate ischemia-induced revascularization and angiogenesis by secretion of growth factor–modulating factors
Authors: Nording Henry , Baron Lasse , Sauter Manuela , Lübken Antje , Rawish Elias , Szepanowski Rebecca , von Esebeck Jacob , Sun Ying , Emami Hossein , Meusel Moritz , Saraei Roza , Schanze Nancy , Gorantla Sivahari Prasad , von Bubnoff Nikolas , Geisler Tobias , von Hundelshausen Philipp , Stellos Konstantinos , Marquardt Jens , Sadik Christian D. , Köhl Jörg , Duerschmied Daniel , Kleinschnitz Christoph , Langer Harald F. 
Published: May 2023

Abstract
In ischemic tissue, platelets can modulate angiogenesis. The specific factors influencing this function, however, are poorly understood. Here, we characterized the complement anaphylatoxin C5a-mediated activation of C5a receptor 1 (C5aR1) expressed on platelets as a potent regulator of ischemia-driven revascularization. We assessed the relevance of the anaphylatoxin receptor C5aR1 on platelets in patients with coronary artery disease as well as those with peripheral artery disease and used genetic mouse models to characterize its significance for ischemia and growth factor–driven revascularization. The presence of C5aR1-expressing platelets was increased in the hindlimb ischemia model. Ischemia-driven angiogenesis was significantly improved in C5aR1−/− mice but not in C5−/− mice, suggesting a specific role of C5aR1. Experiments using the supernatant of C5a-stimulated platelets suggested a paracrine mechanism of angiogenesis inhibition by platelets by means of antiangiogenic CXC chemokine ligand 4 (CXCL4, PF4). Lineage-specific C5aR1 deletion verified that the secretion of CXCL4 depends on C5aR1 ligation on platelets. Using C5aR1−/−CXCL4−/− mice, we observed no additional effect in the revascularization response, underscoring a strong dependence of CXCL4 secretion on the C5a-C5aR1-axis. We identified a novel mechanism for inhibition of neovascularization via platelet C5aR1, which was mediated by the release of antiangiogenic CXCL4.
Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics
Authors: Liu Yingke , Wei Yilin , Dou Yaqing , Li Chenlei , Song Chenglei , Zhang Zhe , Qi Kunlong , Li Xinjian , Qiao Ruimin , Wang Kejun , Li Xiuling , Yang Feng , Han Xuelei 
Published: May 2023

AbstractAs one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor, tenderness, and juiciness of pork. Therefore, it is of great significance to study the mechanism of porcine IMF deposition. Previous research indicated that miR-149-5p promoted the proliferation of porcine intramuscular (IM) preadipocytes and decreased their ability to differentiate, albeit the exact mechanism of action is unknown. In vitro, foreign pigs showed increased miR-149-5p expression and reduced fat deposition when compared to Queshan Black pigs. This study conducted metabolomics and transcriptomics analyses of porcine IM preadipocytes overexpressing miR-149-5p to verify their effects on lipid formation. According to metabolomics analysis, the overexpression of miR-149-5p has significantly altered the lipid, organic acid, and organic oxygen metabolites of porcine IM preadipocytes. Specially speaking, it has changed 115 metabolites, including 105 up-regulated and 10 down-regulated ones, as well as the composition of lipid, organic acid, and organic oxygen metabolism-related metabolites. RNA-seq analysis showed that overexpression of miR-149-5p significantly altered 857 genes, of which 442 were up-regulated, and 415 were down-regulated, with enrichment to MAPK, IL-17, PI3K-Akt, and ErbB signaling pathways. We found that overexpression of miR-149-5p inhibited adipogenic differentiation by changing cAMP signaling pathway in porcine IM preadipocytes. In addition, the overexpression of miR-149-5p may affect the transport of Cu2+ by targeting ATP7A and inhibiting adipogenic differentiation. These findings elucidate the regulatory function of miR-149-5p in porcine IM preadipocytes, which may be a key target for controlling pork quality.
Metagenome-mining indicates an association between bacteriocin presence and strain diversity in the infant gut
Authors: Ormaasen Ida , Rudi Knut , Diep Dzung B. , Snipen Lars 
Published: May 2023

AbstractBackgroundOur knowledge about the ecological role of bacterial antimicrobial peptides (bacteriocins) in the human gut is limited, particularly in relation to their role in the diversification of the gut microbiota during early life. The aim of this paper was therefore to address associations between bacteriocins and bacterial diversity in the human gut microbiota. To investigate this, we did an extensive screening of 2564 healthy human gut metagenomes for the presence of predicted bacteriocin-encoding genes, comparing bacteriocin gene presence to strain diversity and age.ResultsWe found that the abundance of bacteriocin genes was significantly higher in infant-like metagenomes (< 2 years) compared to adult-like metagenomes (2–107 years). By comparing infant-like metagenomes with and without a given bacteriocin, we found that bacteriocin presence was associated with increased strain diversities.ConclusionsOur findings indicate that bacteriocins may play a role in the strain diversification during the infant gut microbiota establishment.
Constructing ferroptosis-related competing endogenous RNA networks and exploring potential biomarkers correlated with immune infiltration cells in asthma using combinative bioinformatics strategy
Authors: Chen Shao-Tian , Yang Nan 
Published: May 2023

Abstract
Background
Asthma is a common chronic respiratory disease worldwide. Recent studies have revealed the critical effects of the ceRNA network and ferroptosis on patients with asthma. Thus, this study aimed to explore the potential ferroptosis-related ceRNA network, investigate the immune cell infiltration level in asthma through integrated analysis of public asthma microarray datasets, and find suitable diagnostic biomarkers for asthma.

Methods
First, three asthma-related datasets which were downloaded from the Gene Expression Omnibus (GEO) database were integrated into one pooled dataset after correcting for batch effects. Next, we screened differentially expressed lncRNAs (DElncRNAs) between patients and healthy subjects, constructed a ceRNA network using the StarBase database and screened ferroptosis–related genes from the predicted target mRNAs for Disease Ontology (DO), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. We also performed Gene Set Enrichment Analysis (GSEA) and Gene Set Variation Analysis (GSVA) on the batch effect-corrected mRNA expression profile. Then, Least Absolute Shrinkage and Selection Operator (LASSO) regression was used to screen potential diagnostic biomarkers, and the diagnostic efficacy was assessed using a receiver operating characteristic (ROC) curve. Finally, we determined the proportion of 22 immune cells in patients with asthma using CIBERSORT and investigated the correlation between key RNAs and immune cells.

Results
We obtained 19 DElncRNAs, of which only LUCAT1 and MIR222HG had corresponding target miRNAs. The differentially expressed ferroptosis-related genes were involved in multiple programmed cell death-related pathways. We also found that the mRNA expression profile was primarily enriched in innate immune system responses. We screened seven candidate diagnostic biomarkers for asthma using LASSO regression (namely, BCL10, CD300E, IER2, MMP13, OAF, TBC1D3, and TMEM151A), among which the area under the curve (AUC) value for CD300E and IER2 were 0.722 and 0.856, respectively. Finally, we revealed the infiltration ratio of different immune cells in asthma and found a correlation between LUCAT1, MIR222HG, CD300E, and IER2 with some immune cells.

Conclusion
This study explored a potential lncRNA-miRNA-mRNA regulatory network and its underlying diagnostic biomarkers (CD300E and IER2) in asthma and identified the immune cells most associated with them, providing possible diagnostic markers and immunotherapeutic targets for asthma.
A preliminary study investigating the neglected domain of mental health in Australian lifesavers and lifeguards
Authors: Fien Samantha , Lawes Jasmin C. , Ledger Jessica , Drummond Murray , Simon Pamela , Joseph Nancy , Daw Shane , Best Talitha , Stanton Robert , de Terte Ian 
Published: May 2023

Abstract
Background
Surf lifesavers and lifeguards have provided essential education, preventative, and rescue services to the Australian community for over 110 years. In this first responder role, surf lifesavers and lifeguards are inadvertently exposed to high risk and trauma related experiences, which may negatively impact mental well-being. To date however, there has been limited research into the mental health of surf lifesavers and lifeguards, and no studies at all on the mental health of adolescent surf lifesavers. The preliminary study aimed to measure the exposure of potentially traumatic events (PTEs), post-traumatic stress symptoms (PTSS), self-efficacy, social support, and attitudes towards mental health problems in Surf Life Saving (SLS) members.

Methods
An anonymous, online survey was developed (adolescent and adult versions) and created to measure the domain of mental health in surf lifesavers and lifeguards. Pearson’s correlations investigated relationships between PTEs, PTSS, self-efficacy, social support, attitudes towards mental health problems, age, years as a SLS member, and years patrolling. Spearman’s Rank was used for violations of normality.

Results
A total of 57 surf lifesavers/lifeguards aged 13–59 years were included in the final analysis. There was a significant positive relationship between exposure to direct trauma and PTSS, which in turn, were associated with greater negative attitudes towards mental health problems towards the mental health of others, and lower levels of self-efficacy. Male and female adults with PTSS reported lower social support, whereas for adolescent males, a positive relationship between direct trauma and PTSS was observed.

Conclusion
This research is the first to explore the mental health of Australian surf lifesavers and lifeguards. The results highlight the potential risks to mental health and well-being associated with this first responder role. More research to protect the vulnerability of this population is warranted.