bluuebunny/crossref_metadata_2025_split huggingface dataset:
The numerical prediction of the Earth’s polar motion based on an advanced multivariate algorithm
Authors: Shi Kunpeng , Ding Hao , Chen Tao , Zou Chuanyi 
Published: March 2023

Since there are complicated changes in the polar motion (PM) from sub-annual to decadal, precisely predicting it is challenging. Here, we provide an advanced multivariate algorithm by combining an iterative oblique singular spectrum analysis (IOSSA) with pseudo data (IOSSApd) and consider more periodic and quasi-periodic signals, especially long-period oscillations (Ding et al., Geophys. Res. Lett., 2019, 46, 13765–13774) and multi-frequency Chandler wobble (Pan, International Journal of Geosciences, 2012, 3, 930–951), than previous studies. The IOSSA in oblique coordinates, due to its weak separability conditions, has a better separation performance than general singular spectrum analysis (SSA), and the IOSSApd approach further solved the shift problem. Upon using the IOSSApd method, the PM data can be separated into deterministic and stochastic components, extrapolated by the multiple-harmonic (MH) and autoregressive integrated moving average (ARIMA) models, respectively. Based on the IERS EOPC04 PM series, we produced multiple sets of PM predictions with a 1-year leading time and reported the IERS Bulletin A predictions as a comparison. For 90-day leading time predictions, the mean absolute errors (MAEs) of the x- and y-components were 7.69 and 5.12 mas, respectively, while the corresponding MAEs obtained by IERS Bulletin A were 9.45 and 5.69 mas, respectively. For up to 360 days, our algorithm obtains the MAEs of PM slowly accumulating to 12.98 mas on average, far better than the 19.14 mas for Bulletin A’s predictions (also significantly superior to the corresponding results given by previous studies). The prediction performance in the middle- and long-term prediction is further compared against the general SSA predictor. By virtue of weak periodic error, our results show that combining the IOSSApd + MH + ARIMA models improved the prediction success rate up to 75.39% and 69.58% for the x- and y-component, respectively.
Prevalence of foodborne viruses and influenza A virus from poultry processing plants to retailed chickens
Authors: Yeo Daseul , Song Mengxiao , Hossain Md. Iqbal , Jung Soontag , Wang Zhaoqi , Seo Dong Joo , Rhee Min Suk , Choi Changsun 
Published: March 2023

IntroductionFoodborne viruses are a serious concern in public health. This study investigated the prevalence of eight foodborne viruses norovirus (NoV), adenovirus (AdV), sapovirus (SapoV), astrovirus, hepatitis A virus (HAV), hepatitis E virus (HEV), rotavirus, aichivirus, and influenza A virus (IAV).Material and methodA total of 316 chicken samples were collected from three poultry processing plants to commercial markets (local and online). RT-qPCR- and PCR-positive amplicons obtained from monitoring were confirmed by sequence analysis.ResultsFoodborne viruses and IAV were not found in poultry processing plants. Of the 100 chickens purchased from the local and online markets, 19 (19.0%) AdV and 2 (2.0%) SapoV were detected. NoV, astrovirus, HAV, HEV, rotavirus, aichivirus, and IAV were not detected in the retailed chickens. Phylogenetic analysis identified 18 human AdV-41, one porcine AdV, and two SapoV-GI.1. It was the first case of the discovery of the SapoV gene in chicken. The average contamination level of detected AdV was 2.4 log DNA copies/g, but there were cases where the highest level was 5.35 log DNA copies/g.DiscussionThis study highlights the importance of chicken's contribution to the transmission of AdV with the possibility of annual variability with emerging symptoms. The prevention of AdV contamination in the food chain from slaughterhouses to retail markets should be monitored and controlled in further study.
Surgical treatment of patellar dislocation: A network meta-analysis of randomized control trials and cohort studies
Authors: Fang Mingqing , Cai Zijun , Pan Linyuan , Ding Yilan , Zhang Yueyao , Cheng Siyuan , Wang Yifan , Gao Jialin , Li Yusheng , Xiao Wenfeng 
Published: March 2023

BackgroundCurrently, there are many surgical options for patellar dislocation. The purpose of this study is to perform a network meta-analysis of the randomized controlled trials (RCTs) and cohort studies to determine the better treatment.MethodWe searched the Pubmed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, clinicaltrials.gov and who.int/trialsearch. Clinical outcomes included Kujala score, Lysholm score, International Knee Documentation Committee (IKDC) score, redislocation or recurrent instability. We conducted pairwise meta-analysis and network meta-analysis respectively using the frequentist model to compare the clinical outcomes.ResultsThere were 10 RCTs and 2 cohort studies with a total of 774 patients included in our study. In network meta-analysis, double-bundle medial patellofemoral ligament reconstruction (DB-MPFLR) achieved good results on functional scores. According to the surface under the cumulative ranking (SUCRA), DB-MPFLR had the highest probabilities of their protective effects on outcomes of Kujala score (SUCRA 96.5 %), IKDC score (SUCRA 100.0%) and redislocation (SUCRA 67.8%). However, DB-MPFLR (SUCRA 84.6%) comes second to SB-MPFLR (SUCRA 90.4%) in Lyshlom score. It is (SUCRA 70%) also inferior to vastus medialis plasty (VM-plasty) (SUCRA 81.9%) in preventing Recurrent instability. The results of subgroup analysis were similar.ConclusionOur study demonstrated that MPFLR showed better functional scores than other surgical options.
Comprehensive comparison of three techniques for the treatment of adjacent segment degeneration after lumbar fusion
Authors: Li Tao , He Hang , Zhang Tonghui , Li Xugui , Xie Wei , Huang Biwang , Xu Feng , Xiong Chengjie 
Published: March 2023

PurposeAdjacent segment degeneration (ASD) following lumbar fusion is technically challenging for spine surgeons. Posterolateral open fusion surgery with pedicle screw fixation is an effective way to treat symptomatic ASD with favorable clinical outcomes; however, it is associated with an increased morbidity rate. Therefore, minimally invasive spine surgery is advocated. This study was designed to compare clinical outcomes among patients with symptomatic ASD who underwent percutaneous transforaminal endoscopic discectomy (PTED) with the transforaminal approach, posterior lumbar interbody fusion (PLIF) with cortical bone trajectory screw fixation (CBT-PLIF), and PLIF with traditional trajectory screw fixation (TT-PLIF).MethodsA retrospective study was conductedon 46 patients (26 men and 20 women; average age 60.8 ± 6.78 years) with symptomatic ASD. The patients were treated with three approaches. The operation time, incision length, time to return to work, complications, and the like were compared among three groups. Intervertebral disc (IVD) space height, angular motion, and vertebral slippage were obtained to assess spine biomechanical stability following surgery. The visual analog scale (VAS) score and Oswestry disability index were evaluated at preoperation and 1-week, 3-month, and the latest follow-ups. Clinical global outcomes were also estimated using modified MacNab criteria.ResultsThe operation time, incision length, intraoperative blood loss, and time to return to work for the PTED group were significantly decreased compared with those for the other two groups (P &lt; 0.05). The radiological indicators in the CBT-PLIF group and TT-PLIF group had better biomechanical stability compared with those in the PTED groups at the latest follow-up (P &lt; 0.05). The back pain VAS score in the CBT-PLIF group was significantly decreased compared with those in the other two groups at the latest follow-up (P &lt; 0.05). The good-to-excellent rate was 82.35% in the PTED group, 88.89% in the CBT-PLIF group, and 85.00% in the TT-PLIF group. No serious complications were encountered. Two patients experienced dysesthesia in the PTED group; screw malposition was found in one patient in the CBT-PLIF group. One case with a dural matter tear was observed in the TT-PLIF group.ConclusionAll three approaches can treat patients with symptomatic ASD efficiently and safely. Functional recovery was more accelerated in the PTED group compared with the other approaches in the short term; CBT-PLIF and TT-PLIF can provide superior biomechanical stability to the lumbosacral spine following decompression compared with PTED; however, compared with TT-PLIF, CBT-PLIF can significantly reduce back pain caused by iatrogenic muscle injury and improve functional recovery. Therefore, superior clinical outcomes were achieved in the CBT-PLIF group compared with the PTED and TT-PLIF groups in the long term.
Simultaneous arthroscopic cystectomy and unicompartmental knee arthroplasty for the management of partial knee osteoarthritis with a popliteal cyst: A case report
Authors: Liu Cai , Zhou Dejie , Liu Xinwei , Huang Jin , Fang Jianguo , Zhou Hongyu , Luo Jianjun , Luo Yiqian , Zhao Lianghu 
Published: March 2023

IntroductionPopliteal cysts are secondary to degenerative changes in the knee joint. After total knee arthroplasty (TKA), 56.7% of patients with popliteal cysts at 4.9 years follow-up remained symptomatic in the popliteal area. However, the result of simultaneous arthroscopic cystectomy and unicompartmental knee arthroplasty (UKA) was uncertain.Case presentationA 57-year-old man was admitted to our hospital with severe pain and swelling in his left knee and the popliteal area. He was diagnosed with severe medial unicompartmental knee osteoarthritis (KOA) with a symptomatic popliteal cyst. Subsequently, arthroscopic cystectomy and unicompartmental knee arthroplasty (UKA) were performed simultaneously. A month after the operation, he returned to his normal life. There was no progression in the lateral compartment of the left knee and no recurrence of the popliteal cyst at the 1-year follow-up.ConclusionFor KOA patients with a popliteal cyst seeking UKA, simultaneous arthroscopic cystectomy and UKA are feasible with great success if managed appropriately.
Risk factors for deep vein thrombosis in patients with pelvic or lower-extremity fractures in the emergency intensive care unit
Authors: Shi Dongcheng , Bao Bingbo , Zheng Xianyou , Wei Haifeng , Zhu Tianhao , Zhang Yi , Zhao Gang 
Published: March 2023

IntroductionThis study aimed to investigate the incidence of deep vein thrombosis (DVT) in patients with pelvic or lower-extremity fractures in the emergency intensive care unit (EICU), explore the independent risk factors for DVT, and investigate the predictive value of the Autar scale for DVT in these patients.MethodsThe clinical data of patients with single fractures of the pelvis, femur, or tibia in the EICU from August 2016 to August 2019 were retrospectively examined. The incidence of DVT was statistically analyzed. Logistic regression was used to analyze the independent risk factors for DVT in these patients. The receiver-operating characteristic (ROC) curve was used to evaluate the predictive value of the Autar scale for the risk of DVT.ResultsA total of 817 patients were enrolled in this study; of these, 142 (17.38%) had DVT. Significant differences were found in the incidence of DVT among the pelvic fractures, femoral fractures, and tibial fractures (P &lt; 0.001). The multivariate logistic regression analysis showed multiple injuries (OR = 2.210, 95% CI: 1.166–4.187, P = 0.015), fracture site (compared with tibia fracture group, femur fracture group OR = 4.839, 95% CI: 2.688–8.711, P &lt; 0.001; pelvic fracture group OR = 2.210, 95% CI: 1.225–3.988, P = 0.008), and Autar score (OR = 1.198, 95% CI: 1.016–1.353, P = 0.004) were independent risk factors for DVT in patients with pelvic or lower-extremity fractures in the EICU. The area under the ROC curve (AUROC) of the Autar score for predicting DVT was 0.606. When the Autar score was set as the cutoff value of 15.5, the sensitivity and specificity for predicting DVT in patients with pelvic or lower-extremity fractures were 45.1% and 70.7%, respectively.DiscussionFracture is a high-risk factor for DVT. Patients with a femoral fracture or multiple injuries have a higher risk of DVT. In the case of no contraindications, DVT prevention measures should be taken for patients with pelvic or lower-extremity fractures. Autar scale has a certain predictive value for the occurrence of DVT in patients with pelvic or lower-extremity fractures, but it is not ideal.
Dietary supplementation with a complex of cinnamaldehyde, carvacrol, and thymol negatively affects the intestinal function in LPS-challenged piglets
Authors: Zhang Yanyan , Li Qian , Wang Zhongxing , Dong Yi , Yi Dan , Wu Tao , Wang Lei , Zhao Di , Hou Yongqing 
Published: March 2023

BackgroundThe effects of cinnamaldehyde, carvacrol and thymol complex (CCT) on the growth performance and intestinal function of piglets challenged with lipopolysaccharide (LPS) were determined. Colistin sulphate (CS) was as a positive control.MethodPiglets (n = 24, 32 days of age) were allocated to four treatments: Control group (fed basal diet), LPS group (fed basal diet), CS+LPS group (fed basal diet + 50 mg/kg CS), and CCT+LPS group (fed basal diet + 50 mg/kg CCT).ResultsResults showed that diarrhea rates of piglets were significantly reduced by CCT and CS supplementation respectively. Further research showed that CS supplementation tended to improve the intestinal absorption function in LPS-challenged piglets. Moreover, CS supplementation significantly reduced the contents of cortisol in blood and malondialdehyde in the duodenum and the activities of inducible nitric oxide synthase in the duodenum and ileum and total nitric oxide synthase in the ileum in LPS-challenged piglets. CS supplementation significantly increased the activities of sucrase in the ileum and myeloperoxidase in the jejunum in LPS-challenged piglets. CS supplementation significantly alleviated the reduced mRNA levels of immune-related genes (IL-4, IL-6, IL-8, IL-10) in mesenteric lymph nodes and jejunum and mucosal growth-related genes (IGF-1, mTOR, ALP) in LPS-challenged piglets. These results suggested that CS supplementation improved the intestinal function in LPS-challenged piglets by improving intestinal oxidative stress, immune stress, and absorption and repair function. However, although CCT supplementation improved oxidative stress by reducing (p &lt; 0.05) the content of malondialdehyde and the activity of nitric oxide synthase in the duodenum, CCT supplementation tended to aggravate the intestinal absorption dysfunction in LPS-challenged piglets. Furthermore, compared with the control and LPS groups, CCT supplementation remarkably elevated the content of prostaglandin in plasma and the mRNA levels of pro-inflammatory factor IL-6 in mesenteric lymph nodes and jejunum, and reduced the activity of maltase in the ileum in LPS-challenged piglets. These results suggested that CCT supplementation had a negative effect on intestinal function by altering intestinal immune stress response and reducing disaccharidase activity in LPS-challenged piglets.ConclusionsCompared to CS, CCT supplementation exhibited a negative effect on intestinal function, suggesting whether CCT can be as an effective feed additive still needs further study.
Establishment and characterization of a multi-drug resistant cell line for canine mammary tumors
Authors: Zhou Chaoyu , Lin Zixiang , Li Xinqiu , Zhang Di , Song Peijia 
Published: March 2023

Background and purposeCanine mammary tumors are the most common tumor disease of female dogs, and adjuvant chemotherapy often results in multi-drug resistance. Currently, the mechanisms underlying the development of tumor multi-drug resistance are unclear. The translation of research applications that can be used to effectively overcome tumor resistance is similarly hampered. Therefore, it is urgent to construct multi-drug resistance models of canine mammary tumors that can be used for research, to explore the mechanisms and means of overcoming resistance.Materials and methodsIn this study, the canine triple negative breast cancer cell line CMT-7364 was induced to develop multidrug resistance using doxorubicin by high-dose drug pulse method. The drug resistance and the expression of drug transport pumps of the cells was verified by CCK8 assay, immunoblotting, qPCR and immunofluorescence. Next, we used scratch assay and Transwell invasion assay to compare the migration and invasion abilities of the two cell lines and examined the expression of EMT-related proteins in both using immunoblotting. The differences of transcriptome between parental and drug-resistant cell lines were detected by RNA-seq sequencing. Finally, mouse xenograft models of drug-resistant and parental cell lines were constructed to evaluate the tumorigenic ability.ResultsAfter more than 50 generations of continuous passages stimulated by high-dose drug pulse method, the morphology of drug-resistant cell line CMT-7364/R tended to be mesenchymal-like and heterogeneous under light microscopy compared with the parental cell line CMT-7364/S, and developed resistance to doxorubicin and other commonly used chemotherapeutic drugs. In CMT-7364/R, BCRP was expressed at higher levels at both transcriptional and protein levels, while P-glycoprotein was not significantly different. Secondly, the migration and invasion ability of CMT-7364/R was significantly enhanced, with decreased expression of E-cadherin and increased expression of vimentin and mucin 1-N terminus. Finally, mouse xenograft models were constructed, while there was no significant difference in the volume of masses formed at 21 days.ConclusionIn summary, by using the canine mammary tumor cell line CMT-7364/S as the parental cell line, we successfully constructed a multidrug-resistant CMT-7364/R with high-dose drug pulse methods. Compared to its parental cell line, CMT-7364/R has decreased growth rate, overexpression of BCRP and increased migration and invasion ability due to EMT. The results of this study showed that CMT-7364/R might serve as a model for future studies on tumor drug resistance.
CRISPR/Cas9 Mediated Fluorescent Tagging of Caenorhabditis elegans SPE-38 Reveals a Complete Localization Pattern in Live Spermatozoa
Authors: Zuo Yamei , Mei Xue , Singson Andrew 
Published: March 2023

The Caenorhabditis elegans spe-38 gene encodes a four-pass transmembrane molecule that is required in sperm for fertilization. In previous work, the localization of the SPE-38 protein was examined using polyclonal antibodies on spermatids and mature amoeboid spermatozoa. SPE-38 is localized to unfused membranous organelles (MOs) in nonmotile spermatids. Different fixation conditions revealed that SPE-38 either localized to fused MOs and the cell body plasma membrane or the pseudopod plasma membrane of mature sperm. To address this localization paradox in mature sperm, CRISPR/Cas9 genome editing was used to tag endogenous SPE-38 with fluorescent wrmScarlet-I. Homozygous male and hermaphrodite worms encoding SPE-38::wrmScarlet-I were fertile indicating the fluorescent tag does not interfere with SPE-38 function during sperm activation or fertilization. We found that SPE-38::wrmScarlet-I localized to MOs in spermatids consistent with previous antibody localization. In mature and motile spermatozoa we found SPE-38::wrmScarlet-I in fused MOs, the cell body plasma membrane, and the pseudopod plasma membrane. We conclude that the localization pattern observed with SPE-38::wrmScarlet-I represents the complete distribution of SPE-38 in mature spermatozoa and this localization pattern is consistent with a hypothesized role of SPE-38 directly in sperm-egg binding and/or fusion.
Reprogramming Effects of Postbiotic Butyrate and Propionate on Maternal High-Fructose Diet-Induced Offspring Hypertension
Authors: Tain You-Lin , Hou Chih-Yao , Chang-Chien Guo-Ping , Lin Sufan , Tzeng Hong-Tai , Lee Wei-Chia , Wu Kay L. H. , Yu Hong-Ren , Chan Julie Y. H. , Hsu Chien-Ning 
Published: March 2023

Maternal nutrition has a key role in the developmental programming of adult disease. Excessive maternal fructose intake contributes to offspring hypertension. Newly discovered evidence supports the idea that early-life gut microbiota are connected to hypertension later in life. Short-chain fatty acids (SCFAs), butyrate, and propionate are microbiota-derived metabolites, also known as postbiotics. The present study aimed to determine whether maternal butyrate or propionate supplementation can protect offspring from hypertension using a maternal high-fructose (HF) diet rat model. Female Sprague Dawley rats were allocated during pregnancy and lactation to (1) regular chow (ND); (2) 60% high-fructose diet (HF); (3) HF diet plus butyrate (HFB, 400 mg/kg/day); and (4) HF diet plus propionate (HFP, 200 mmol/L). Male offspring were sacrificed at 12 weeks of age. The maternal HF diet impaired the offspring’s BP, which was prevented by perinatal butyrate or propionate supplementation. Both butyrate and propionate treatments similarly increased plasma concentrations of propionic acid, isobutyric acid, and valeric acid in adult offspring. Butyrate supplementation had a more profound impact on trimethylamine N-oxide metabolism and nitric oxide parameters. Whilst propionate treatment mainly influenced gut microbiota composition, it directly altered the abundance of genera Anaerovorax, Lactobacillus, Macellibacteroides, and Rothia. Our results shed new light on targeting gut microbiota through the use of postbiotics to prevent maternal HF intake-primed hypertension, a finding worthy of clinical translation.
The Integration of WoT and Edge Computing: Issues and Challenges
Authors: Anees Tayyaba , Habib Qaiser , Al-Shamayleh Ahmad Sami , Khalil Wajeeha , Obaidat Muath A. , Akhunzada Adnan 
Published: March 2023

The Web of Things is an improvement on the Internet of Things (IoT) that incorporates smart objects into both the web architecture (application) and the internet (network). WoT applications are inescapable in residential homes and communities. The intent behind WoT applications is to increase sustainable development for reducing resource consumption. The Web of Things (WoT) aims to create a decentralized Internet of Things. Edge computing addresses IoT computing demands by reducing the escalation in resource congestion situations. In edge computing data is placed closed to the end users which diverts computation load from the centralized data centers. Furthermore, the dispersed structure balances network traffic and minimizes traffic peaks in IoT networks. Therefore, resulting in reducing transmission delays between edge servers and end users which improves response times for real-time WoT applications. Low battery supply to nodes with enough power resources can increase the lifespan of the individual nodes by moving processing and communication overhead from the nodes. This paper integrates WoT and edge computing and compares their functionalities. In addition, it demonstrates how edge computing enhances WoT performance and concentrates on transmission, storage, and computation aspects. Furthermore, for performance evaluation it categorizes edge computing based on different architectures. Moreover, the challenges of Web of Things and edge computing have been discussed in terms of bandwidth, latency, energy, and cost. Finally, advantages of the Web of Things and edge computing have been discussed.