bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological variability or inter‐observer bias? A methodological toolkit to improve data quality of multi‐researcher datasets for the analysis of morphological variation
Authors: Schüßler Dominik , Blanco Marina B. , Guthrie Nicola K. , Sgarlata Gabriele M. , Dammhahn Melanie , Ernest Refaly , Evasoa Mamy Rina , Hasiniaina Alida , Hending Daniel , Jan Fabien , le Pors Barbara , Miller Alex , Olivieri Gillian , Rakotonanahary Ando N. , Rakotondranary Solofomalala Jacques , Rakotondravony Romule , Ralantoharijaona Tantely , Ramananjato Veronarindra , Randrianambinina Blanchard , Raoelinjanakolona Nancia N. , Rasoazanabary Emilienne , Rasoloarison Rodin M. , Rasolofoson David W. , Rasoloharijaona Solofonirina , Rasolondraibe Emmanuel , Roberts Sam Hyde , Teixeira Helena , van Elst Tobias , Johnson Steig E. , Ganzhorn Jörg U. , Chikhi Lounès , Kappeler Peter M. , Louis Edward E. , Salmona Jordi , Radespiel Ute 
Published: August 2023

AbstractObjectivesThe investigation of morphological variation in animals is widely used in taxonomy, ecology, and evolution. Using large datasets for meta‐analyses has dramatically increased, raising concerns about dataset compatibilities and biases introduced by contributions of multiple researchers.Materials and MethodsWe compiled morphological data on 13 variables for 3073 individual mouse lemurs (Cheirogaleidae, Microcebus spp.) from 25 taxa and 153 different sampling locations, measured by 48 different researchers. We introduced and applied a filtering pipeline and quantified improvements in data quality (Shapiro‐Francia statistic, skewness, and excess kurtosis). The filtered dataset was then used to test for genus‐wide sexual size dimorphism and the applicability of Rensch's, Allen's, and Bergmann's rules.ResultsOur pipeline reduced inter‐observer bias (i.e., increased normality of data distributions). Inter‐observer reliability of measurements was notably variable, highlighting the need to reduce data collection biases. Although subtle, we found a consistent pattern of sexual size dimorphism across Microcebus, with females being the larger (but not heavier) sex. Sexual size dimorphism was isometric, providing no support for Rensch's rule. Variations in tail length but not in ear size were consistent with the predictions of Allen's rule. Body mass and length followed a pattern contrary to predictions of Bergmann's rule.DiscussionWe highlighted the usefulness of large multi‐researcher datasets for testing ecological hypotheses after correcting for inter‐observer biases. Using genus‐wide tests, we outlined generalizable patterns of morphological variability across all mouse lemurs. This new methodological toolkit aims to facilitate future large‐scale morphological comparisons for a wide range of taxa and applications.
Sudden Unexpected Death in Epilepsy and Respiratory Defects in a Mouse Model of DEPDC5‐Related Epilepsy
Authors: Kao Hsin‐Yi , Yao Yilong , Yang Tao , Ziobro Julie , Zylinski Mary , Mir Mohd Yaqub , Hu Shuntong , Cao Runnan , Borna Nurun Nahar , Banerjee Rajat , Parent Jack M. , Wang Shuo , Leventhal Daniel K. , Li Peng , Wang Yu 
Published: August 2023

ObjectivesDEPDC5 is a common causative gene in familial focal epilepsy with or without malformations of cortical development. Its pathogenic variants also confer a significantly higher risk for sudden unexpected death in epilepsy (SUDEP), providing opportunities to investigate the pathophysiology intersecting neurodevelopment, epilepsy, and cardiorespiratory function. There is an urgent need to gain a mechanistic understanding of DEPDC5‐related epilepsy and SUDEP, identify biomarkers for patients at high risk, and develop preventive interventions.MethodsDepdc5 was specifically deleted in excitatory or inhibitory neurons in the mouse brain to determine neuronal subtypes that drive epileptogenesis and SUDEP. Electroencephalogram (EEG), cardiac, and respiratory recordings were performed to determine cardiorespiratory phenotypes associated with SUDEP. Baseline respiratory function and the response to hypoxia challenge were also studied in these mice.ResultsDepdc5 deletion in excitatory neurons in cortical layer 5 and dentate gyrus caused frequent generalized tonic–clonic seizures and SUDEP in young adult mice, but Depdc5 deletion in cortical interneurons did not. EEG suppression immediately following ictal offset was observed in fatal and non‐fatal seizures, but low amplitude rhythmic theta frequency activity was lost only in fatal seizures. In addition, these mice developed baseline respiratory dysfunction prior to SUDEP, during which ictal apnea occurred long before terminal cardiac asystole.InterpretationDepdc5 deletion in excitatory neurons is sufficient to cause DEPDC5‐related epilepsy and SUDEP. Ictal apnea and respiratory dysregulation play critical roles in SUDEP. Our study also provides a novel mouse model to investigate the underlying mechanisms of DEPDC5‐related epilepsy and SUDEP. ANN NEUROL 2023;94:812–824
Synthesis and application of styrene butadiene latex powder for cementing
Authors: Zheng Yong , Liao Guoli , Peng Zhigang , Feng Qian 
Published: August 2023

AbstractTo address the problems of brittleness and poor elastic deformation ability of oil‐well cement stone, styrene‐butadiene latex powder was prepared using emulsion polymerization with styrene, liquid polybutadiene, 2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid, and fumaric acid as raw materials. The dispersion properties of styrene‐butadiene latex powder in water were investigated, as well as the elastic enhancement effect of the powder in cement. The test results showed that styrene‐butadiene latex powder has good dispersion performance in water and is not easy to agglomerate, which can improve the fluidity of cement slurry. The elastic modulus of cement stone mixed with styrene butadiene latex powder is 43.1% and 27.8% lower than that of blank cement stone without styrene‐butadiene latex powder and the cement stone mixed with commercially available latex powder, respectively. The results of the mechanism analysis showed that the styrene‐butadiene latex powder can be filled in the cement stone, improved the compactness of the cement stone, buffered the external stress impact, and improve the elasticity of the cement stone.
Dihydromyricetin encapsulated gelatin‐dialdehyde‐β‐cyclodextrin hydrogels for antibacterial application
Authors: Zhang Aomei , Wang Tingting , Huang Feng , Yang Minghui , Jiang Xingxing , Chen Xiang 
Published: August 2023

AbstractDihydromyricetin (DMY), a natural flavonoid compound, has garnered extensive research interests owing to its high pharmaceutical value with antioxidant, anticancer and antimicrobial properties. However, its intrinsic limitation of low water‐solubility and poor stability severely obstructs its biomedical applications. Herein, a dynamic imine cross‐linking strategy to assemble DMY/DA‐β‐CD/gelatin hydrogel (DDG) is reported, with the aim of enhancing DMY bio‐efficacy and achieve a synergistic antibacterial effect. DMY can be wrapped by the amphiphilic dialdehyde‐β‐cyclodextrin (DA‐β‐CD) to form an inclusion compound (DMY/DA‐β‐CD), significantly increasing its water‐solubility without compromising its sustainable release. Furthermore, the Schiff base reaction between DA‐β‐CD and gelatin drive the formation of DDG hydrogel featuring viscoelastic, self‐healing and injectability properties, serving as a DMY delivery platform. DMY and DA‐β‐CD synergistically inhibited bacterial proliferation, enabling a broad‐spectrum antibacterial effect against Escherichia coli and Staphylococcus aureus with over 90% inhibition and good biocompatibility. Our work provides a paradigm for self‐assembling DMY inclusion compound into a hydrogel to improve the bio‐efficacy of DMY, so that the hydrogel can be used as a carrier for hydrophobic drug delivery with promising applications in the biomedical field.
Impact of gross anatomy laboratory on student written examination performance: A 3‐year study of a large‐enrollment undergraduate anatomy course
Authors: Anderson Hana , Weil Jennifer A. , Tucker Richard P. , Gross Douglas S. 
Published: August 2023

AbstractThe efficacy of the various pedagogies that are used in human anatomy laboratories has been extensively debated. Nevertheless, an important question remains relatively unexamined—how the learning experience in the anatomy laboratory impacts students' mastery and application of anatomical knowledge beyond the laboratory setting. In this study, the effect of a prosection‐based anatomy laboratory on overall comprehension and mastery of anatomical knowledge was evaluated in an upper division undergraduate anatomy curriculum that consists of a mandatory lecture course and an optional laboratory course. This flexible curricular structure permitted assessing the merit of laboratory learning on the written examination performance of the lecture course. In 2019 and 2022, the anatomy laboratory was taught in‐person using prosections, while in 2021 due to the Covid‐19 pandemic related regulations, it was taught remotely with live‐streaming of prosections using document cameras. In both in‐person and remote instructive formats, written examination scores of the lecture course were compared between two cohorts of students: Those enrolled in lecture only and those enrolled in both lecture and laboratory. Results showed that the cohort enrolled in both lecture and laboratory courses consistently outperformed the lecture‐only cohort by one full letter grade. Furthermore, when the degrees of improvement on written examination scores were compared between the two instructive formats, in‐person laboratory had a greater increase compared to remote laboratory. Altogether this study demonstrates that the prosection‐based anatomy laboratory enhances students' mastery of anatomical knowledge beyond the laboratory setting by promoting comprehension of spatial relationships of anatomical structures.
Adaptive neural practical fixed‐time command filtered control for multi‐input and multi‐output nonlinear systems with dead zones input and unknown control direction
Authors: Kang Shijia , Xiaoping Liu Peter , Wang Huanqing 
Published: August 2023

AbstractIn this article, under the circumstance of dead zones input and unknown control direction, the adaptive practical fixed‐time control strategy is presented for a general class of multi‐input and multi‐output (MIMO) nonlinear systems. The inherent explosion of computational complexity difficulty is eliminated by adopting a command filter technique and the universal approximation properties of radial basis function neural networks (RBFNNs) are applied to model the unknown nonlinear functions. The difficulties of the dynamic surface method and unknown directions can be handled by invoking error compensation mechanism and Nussbaum‐type functions, respectively. The uniqueness of the presented control scheme is that the tracking system can achieve the fixed‐time stability without relying on the boundedness of dead‐zone parameters. The fixed‐time convergence of the output tracking error and the semiglobally fixed‐time stable of closed‐loop system are assured via the developed adaptive fixed‐time command filtered controller. Finally, a practical example is supplied to further validate the availability of the presented theoretic result.
Bericht des Arbeitskreises „Baugruben“: Tragfähigkeit von Steifen (EB 52) und Eurocode 7
Authors: Hettler Achim , Kinzler Steffen , Hackenbroch Wilfried 
Published: August 2023

AbstractDer Arbeitskreis „Baugruben“ der Deutschen Gesellschaft für Geotechnik (DGGT) veröffentlicht seit 1968 regelmäßig Empfehlungen für die Berechnung, Bemessung und Konstruktion von Baugrubenumschließungen. Der vorliegende Beitrag gibt einen Überblick über die aktuellen Diskussionen im Arbeitskreis. Im vergangenen Jahr erfolgte in der Hauptsache die Mitwirkung bei der Kommentierung der Entwürfe des neuen Eurocode 7. Ein weiterer Schwerpunkt war die Überarbeitung der EB 52 „Tragfähigkeit von Steifen“.
Reduced iron and cobalt levels in response to curcumin supplementation are not responsible for the prolonged larval development and do not affect the oxidative stress tolerance and polyamine status of D. melanogaster
Authors: Lüersen Kai , Jöckel Tobias , Chin Dawn , Demetrowitsch Tobias , Schwarz Karin , Rimbach Gerald 
Published: August 2023

AbstractRecent reports indicated that the phytochemical curcumin possesses iron‐chelating activity. Here, by employing the fruit fly Drosophila melanogaster, we conducted feeding studies supplementing curcumin or, as a control, the iron chelator bathophenanthroline (BPA). First, the absorption and further metabolization of dietary curcuminoids were proved by metabolomics analyses. Next, we found that 0.2% dietary curcumin, similar to BPA, lowered the iron but also the cobalt content, and to a lesser extent affected the manganese and zinc status. Supplementation during larval stages was required and sufficient for both compounds to elicit these alterations in adult animals. However, curcumin‐induced retarded larval development was not attributable to the changed trace metal status. In addition, a reduction in the iron content of up to 70% by curcumin or BPA supplementation did not reduce heme‐dependent catalase activity and tolerance toward H2O2 in D. melanogaster. Moreover, polyamines were not influenced by curcumin treatment and decreased iron levels. This was confirmed for selected organs from 0.2% curcumin‐treated mice, except for the spleen. Here, elevated spermidine level and concomitant upregulation of genes involved in polyamine production were associated with a putatively anemia‐derived increased spleen mass. Our data underline that the metal‐chelating property of curcumin needs to be considered in feeding studies.
Industry 4.0 deployment for circular economy performance—Understanding the role of green procurement and remanufacturing activities
Authors: Sahoo Saumyaranjan , Jakhar Suresh Kumar 
Published: August 2023

AbstractAs regulatory authorities emphasize sustainable practices, manufacturing organizations rely on Industry 4.0 technologies to shift their business model from a linear to a circular economy. While intuitively this transformation seems straightforward, in reality, it is rather nuanced, thereby necessitating scholarly investigation into process‐based antecedents of circular economy performance of organizations. This study used structural equation modeling to analyze empirical data from 278 Indian manufacturers. Largely, the results demonstrate that the adoption of Industry 4.0 technologies does have a significant effect on the performance of the circular economy within these organizations, owing to their strong influence on green procurement and remanufacturing activities. Additionally, the results show that these process activities (e.g., green procurement and remanufacturing) do mediate the interaction between the deployment of Industry 4.0 and circular economy performance.
Theory‐based behavior change intervention to increase uptake of risk‐reducing salpingo‐oophorectomy in women with a BRCA1 or BRCA2 pathogenic variant: The PREVENT randomized controlled trial
Authors: Metcalfe Kelly A. , Pal Tuya , Narod Steven A. , Armel Susan , Shickh Salma , Buckley Kathleen , Walters Scott T. , Brennenstuhl Sarah , Kinney Anita Y. 
Published: August 2023

AbstractObjectiveTo evaluate the effect of a theory‐based behavioral intervention delivered by genetic counselors on the uptake of risk‐reducing salpingo‐oophorectomy (RRSO) at 12 and 24 months by women with a BRCA1 or BRCA2 pathogenic variant (PV) compared to women who received usual care.MethodsIn this two‐arm, multi‐site randomized controlled trial participants were randomized to receive a theoretically‐guided behavioral telephone intervention or usual care. Outcome data were collected at 12 and 24 months. Participants in the usual care arm were offered the intervention after 12 months.ResultsData on 107 participants were included in the analysis. There was no significant difference in the proportion of women who had a RRSO by 1 year (28.6%‐ intervention; 22.9%‐ usual care (p = 0.54)). At 1 year, women who received the intervention had significantly lower mean decisional conflict (pinteraction <0.001) and a higher mean knowledge score at one‐year compared to usual care (pinteraction <0.001). At 2 years, 53.9% of participants in the intervention arm had RRSO compared to 32.6% in usual care (p = 0.05).ConclusionsA theory‐based behavioral intervention delivered by genetic counselors to women with a BRCA PV who chose not to have the recommended RRSO was effective at reducing decisional conflict and increasing knowledge in women with a BRCA1 or BRCA2 PV.
Use of a mixed‐methods approach to develop a guidebook with messaging to encourage colorectal cancer screening among Black individuals 45 and older
Authors: Anyane‐Yeboa Adjoa , Aubertine Michelle , Parker Aisha , Sylvester Kaitlin , Levell Caleb , Bell Emily , Emmons Karen M. , May Folasade P. 
Published: August 2023

AbstractIntroductionColorectal cancer (CRC) is the second leading cause of cancer‐related deaths in the United States and disproportionately impacts Black individuals. Here, we describe the mixed‐methods approach used to develop a tailored message guidebook to promote CRC screening among Black individuals in the setting of recently updated screening guidelines.MethodsThis mixed‐methods study included 10 in‐depth qualitative interviews and 490 surveys in a nationally representative sample of unscreened Black individuals age ≥ 45. Messages were developed based on American Cancer Society (ACS) and National Colorectal Cancer Roundtable (NCCRT) research findings, tested among Black individuals using MaxDiff analytic methods, and reviewed by a multi‐sector expert advisory committee of NCCRT members.ResultsThe most frequently reported screening barrier in all age groups was self‐reported procrastination (40.0% in age 45–49, 42.8% for age 50–54, 34.2% for age ≥ 55). Reasons for procrastination varied by age and included financial concerns, COVID‐19 concerns, and fear of the test and bowel preparation. Additional screening barriers included lack of symptoms, provider recommendation, and family history of CRC. Most individuals age 45–49 preferred to receive screening information from a healthcare provider (57.5%); however, only 20% reported that a provider had initiated a screening conversation.ConclusionsWe identified age‐specific barriers to CRC screening and tailored messaging to motivate participation among unscreened Black people age ≥ 45. Findings informed the development of the NCCRT and ACS guidebook for organizations and institutions aiming to increase CRC screening participation in Black individuals.
A systematic review of the effectiveness of patient‐initiated follow‐up after cancer
Authors: Dretzke Janine , Chaudri Talhah , Balaji Rishab , Mehanna Hisham , Nankivell Paul , Moore David J. ,   Members of the PETNECK2 Research Team ‐ in alphabetical order by first name
Published: August 2023

AbstractBackgroundThe traditional cancer follow‐up (FU) model for cancer survivors is by scheduled clinic appointments; however, this is not tailored to patient needs and is becoming unsustainable. Patient‐initiated follow‐up (PIFU) may be a more effective and flexible alternative. This systematic review aims to analyse all existing evidence from randomised controlled trials (RCTs) on the effectiveness of PIFU compared with other FU models that include routinely scheduled appointments in adults who have been treated with curative intent for any type of cancer.MethodsStandard systematic review methodology aimed at limiting bias was used for study identification, selection and data extraction. MEDLINE, Embase, CINAHL, the Cochrane Database of Systematic Reviews and Epistemonikos were searched for systematic reviews to March 2022, and Cochrane CENTRAL was searched for RCTs from 2018 (April 2023). Ongoing trial registers were searched (WHO ICTRP, ClinicalTrials.gov, April 2023). Eligible studies were randomised controlled trials comparing PIFU with an alternative FU model in adult cancer survivors. Risk of bias assessment was via the Cochrane risk of bias tool‐2. Meta‐analysis was precluded by clinical heterogeneity and results were reported narratively.ResultsTen RCTs were included (six breast, two colorectal, one endometrial cancer and one melanoma, total n = 1754); all studies had risk of bias concerns, particularly relating to how missing data were handled, and populations were unlikely to be representative. Limited findings in breast cancer suggested that type of FU does not affect recurrence detection or patient‐related outcomes, while PIFU may reduce the number of clinic visits. Adding patient‐led surveillance to routine FU may increase melanoma detection. Evidence for other types of cancer is too limited to draw firm conclusions.ConclusionsPIFU may be a viable FU model in breast cancer, but further research is needed for other types of cancer and on long‐term outcomes. A protocol was registered with PROSPERO (CRD42020181424).
Structurally Diverse Metabolites from the Marine‐Derived Streptomyces sp. DS‐27 Based on Two Different Culture Conditions
Authors: Yan Feihang , Fang Jiebin , Ding Wanjing , Tang Xinyi , Chen Xiaoming , Ma Zhongjun , Wang Jinhui 
Published: August 2023

AbstractNine new compounds, including streptothiomycin A−E (1–5), two cyclopentenones (6, 7), one α‐pyrone (8), wailupemycin Q (20), along with sixteen known compounds were identified from a rhizosphere strain Streptomyces sp. DS‐27 derived from the marine cordgrass Spartina alterniflora under two different culture conditions. All of the structures were elucidated by extensive analysis of 1D/2D NMR and HR‐ESI‐MS data. The absolute configurations were determined by NOESY analysis, ECD, specific rotation and GIAO NMR calculations, and DP4+ probability analysis. Bioactivity investigation showed that compounds 5 and 7 exhibited significant inhibitory effects on LPS‐induced NO production in a dose‐dependent manner, which indicates their anti‐inflammatory potential.
G1P3/IFI6, an interferon stimulated protein, promotes the association of RAB5+ endosomes with mitochondria in breast cancer cells
Authors: Davenport Anne M. , Morris Madeleine , Sabti Fatima , Sabti Sarah , Shakya Diksha , Hynds DiAnna L. , Cheriyath Venugopalan 
Published: August 2023

AbstractG1P3/IFI6 is an interferon stimulated gene with antiapoptotic, prometastatic, and antiviral functions. Despite its pleiotropic functions, subcellular localization of G1P3 remains unclear. Using biochemical‐ and confocal microscopic approaches, this study identified the localization of G1P3 in organelles of the endomembrane system and in the mitochondria of breast cancer cells. In cell fractionation studies, both interferon‐induced endogenous‐ and stably expressed G1P3 cofractionated with affinity‐isolated mitochondria. Results of the protease protection assay have suggested that ~24% of mitochondrial G1P3 resides within the mitochondria. Conforming to this, confocal microscopy studies of cells stably expressing epitope‐tagged G1P3 (MCF‐7/G1P3‐FLAG), identified its localization in mitochondria (~38%) as well as in ER, trans‐Golgi network (TGN), lysosomes, and in RAB5 positive (RAB5+) endosomes. These results suggested the trafficking of G1P3 from TGN into endolysosomes. Both G1P3 and RAB5 were known to confer apoptosis resistance through mitochondrial stabilization. Therefore, the effects of G1P3 on the localization of RAB5 in mitochondria were tested. Compared to vector control, the co‐occurrence of RAB5 with the mitochondria was increased by 1.5‐fold in MCF‐7/G1P3‐FLAG expressing cells (p ≤ .005). Taken together, our results demonstrate a role for G1P3 to promote the association of RAB5+ endosomes with mitochondria and provide insight into yet another mechanism of G1P3‐induced cancer cell survival.