bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of HAND2 Expression by LncRNA HAND2-AS1 in Ovarian Endometriosis Involving DNA Methylation
Authors: Liu Lingli , Dong Huijing , Guan Yining , Fan Tingting , Sun Wenxia , Bagchi Indrani C , Miao Congxiu , Li Quanxi 
Published: April 2023

Abstract
HAND2 is a critical mediator of progesterone receptor signaling in endometrium. Silencing of HAND2 expression is associated with female infertility and endometrial cancers. We recently observed that lncRNA HAND2-AS1 and HAND2 are expressed coordinately in human endometrial stromal cells. To investigate involvement of HAND2-AS1 and HAND2 in pathogenesis of endometriosis, we employed immunohistochemistry, in situ hybridization, and quantitative real-time PCR to assess their expression in normal endometrium and the ectopic lesions obtained from patients with ovarian endometriosis. HAND2 promoter methylation was also monitored in these samples. Our results revealed that HAND2 and HAND2-AS1 expression levels were reduced but promoter methylation was enhanced significantly in ectopic endometrium when compared with the normal controls. Fluorescence in situ hybridization showed that HAND-AS1 is predominantly localized in the nuclei of endometrial stromal cells in contrast to the cytoplasmic distribution in epithelial cell compartment. To further investigate regulation of HAND2 expression by HAND2-AS1, HAND2-AS1 was silenced or overexpressed in human endometrial stromal cells. Our studies showed that expression levels of HAND2 and its direct target IL15 were attenuated markedly in HAND2-AS1 silenced cells but enhanced significantly in the overexpressed human endometrial stromal cells. Silencing of HAND2-AS1 also impaired endometrial stromal cell decidualization as indicated by downregulation of decidual biomarkers IGFBP1 and PRL. In addition, HAND2 promoter methylation was also enhanced upon HAND2-AS1 silencing. RNA immunoprecipitation studies further revealed that HAND2-AS1 is capable of binding to DNA methyltransferase DNMT1, indicating that HAND2-AS1 governs HAND2 expression epigenetically involving DNA methylation.
Diffuse Isocitrate Dehydrogenase–Mutant Gliomas With Histone H3 Alterations Are Distinguished by Unique Clinical Characteristics, Molecular Expression Profile, and Survival Prognosis
Authors: Cheng Cheng , Wang Di , Yu Mingchen , Zhai You , Pan Changqing , Liang Bo , Zhang Jiazheng , Wang Chen , Yin Yiyun , Li Lianwang , Wu Fan , Shi Zhongfang , Fan Xing , Liu Xing , Wang Zhiliang , Zhao Zheng , Li Guanzhang , Jiang Tao , Zhang Wei 
Published: April 2023

BACKGROUND AND OBJECTIVES:
Histopathological features and molecular biomarkers have been studied as potential prognostic factors. This study aimed to investigate the clinical features, molecular phenotypes, and survival prognosis of isocitrate dehydrogenase (IDH)-mutant (IDHmt) gliomas with histone H3 alterations (H3-alterations).

METHODS:
A total of 236 and 657 patients with whole-exome sequencing data were separately collected from the Chinese Glioma Genome Atlas and The Cancer Genome Atlas databases. Survival analysis of patients with glioma was performed using Kaplan–Meier survival curves stratified by histone H3 status. Univariate and multivariate analyses were used to identify the associations between histone H3 status and other clinicopathological factors with survival in patients with IDH-mutant gliomas.

RESULTS:
Diffuse gliomas with H3 alterations are more likely to be high grade in 2 cohorts (P = .025 and P = .021, respectively). IDHmt glioma patients with H3-alteration had significantly less life expectancy than histone H3 wild-type (P = .041 and P = .008, respectively). In the Chinese Glioma Genome Atlas cohort, Karnofsky performance scores ≤ 80 (HR 2.394, 95% CI 1.257-4.559, P = .008), extent of resection (HR 0.971, 95% CI 0.957-0.986, P < .001), high WHO grade (HR 6.938, 95% CI 2.787-17.269, P < .001), H3-alteration (HR 2.482, 95% CI 1.183-4.981, P = .016), and 1p/19q codeletion (HR 0.169, 95% CI 0.073-0.390, P < .001) were independently associated with IDHmt gliomas. In the The Cancer Genome Atlas cohort, age (HR 1.034, 95% CI 1.008-1.061, P = .010), high WHO grade (HR 2.365, 95% CI 1.263-4.427, P = .007), and H3-alteration (HR 2.501, 95% CI 1.312-4.766, P = .005) were independently associated with IDHmt gliomas.

CONCLUSION:
Identification and assessment of histone H3 status in clinical practice might help improve prognostic prediction and develop therapeutic strategies for these patient subgroups.
Combined Endoscopic Endonasal and Transpalatal Repair of Congenital Anterior Cranial Fossa Agenesis
Authors: Yang Nathan , Feldstein Neil A. , Gudis David A. 
Published: April 2023

BACKGROUND:
Congenital basal meningoceles and encephaloceles are rare pathologies that may present in isolation or with characteristic-associated clinical features. Rarely, children with congenital midline defects may present with massive encephaloceles secondary to anterior cranial fossa agenesis. Traditionally, transcranial approaches with frontal craniotomies were used to reduce the herniated contents and repair the skull base defect. However, high rates of morbidity and mortality associated with craniotomies have favored the development and adoption of less-invasive techniques.

OBJECTIVE:
To present a novel technique for combined endoscopic endonasal and transpalatal repair of a giant basal meningocele through an extensive sphenoethmoidal skull base defect.

METHODS:
A representative case of congenital anterior cranial fossa agenesis with a giant meningocele was selected. Clinical and radiological presentations were reviewed, and the intraoperative surgical technique was documented and recorded.

RESULTS:
A surgical video highlighting each surgical step was included to complement the description of the technique. The surgical outcome from the selected case is also presented.

CONCLUSION:
This report describes a combined endoscopic endonasal and transpalatal approach to repair an extensive anterior skull base defect with herniation of intracranial content. This technique capitalizes on the advantages of each approach to address this complex pathology.
Validation of ageism scale for dental students in India: (Ageism Scale for Dental Students- India) – a cross sectional study.
Authors: Shenoy Ramya , Jodalli Praveen S , Rao B Shushma , Shetty Aishwarya Shodhan , Thomas Manuel , Shigli Kamal , Marchini Leonardo 
Published: April 2023

Background: Ageism persists in many different societies as it is innate and subconscious in nature. Negative effects such as loneliness, mistreatment, and occupational discrimination are frequently present due to ageism. The dental students in our study were wary of the possible benefits of expensive dental care because ageism is rife in their field. There is no validated and reliable ageism scale to assess how dental students perceive ageism in India. The current study was carried out to validate the ageism scale for dental students in the Indian context. Methods: This was a cross sectional analytical study carried out among both males and females in Manipal College of Dental Sciences, Mangalore in which the instrument was 27-item Ageism scale for dental students. Content validity was done by six subject experts. The final version was administered to 213 students/Residents of dental school. The factorability of data was confirmed with KMO = 0.61 and Bartlett’s Test of Sphericity resulting in p < 0.001. Results: Final PCA model resulted in 15 items and six components that together accounted for 70.37% of overall variance. The six components had reliability ranging from marginal 0.51 (Component 6) to a high of 0.81 (Component 3). As per the gender differences by component females showed less ageism than men in “non-compliance” (-0.9(-1.66-0.14), p<0.05) and “practitioner perspective” (1.43 (0.84, 2.03), p<0.01). Statistical significance was seen in Barriers/concerns in dental treatment of elderly where residents showed reversed (1.4 (0.41, 2.38), p<0.01). Urban group showed more ageism for component ‘time restraint’ (-0.79 (-1.57, -0.02), p<0.05. Conclusion: Preliminary validation resulted in 15 item scale with six components with acceptable validity of the ageism scale and could be further tested in large samples. This scale will help recognize ageism in Indian context and provide necessary information to make changes in the curriculum as required.
Pico-tesla magnetic field detection with integrated flux concentrators using a multi-frequency modulation technique on the solid nuclear spin in diamonds
Authors: Zhao Junzhi , Li Yang , Liu Xinyu , Gao Yuxin , Zheng Doudou , Wang Qimeng , Liu Zhenhua , Li Zhonghao , Guo Hao , Yasuhiro Sugawara , Tang Jun , Ma Zongmin , Liu Jun 
Published: April 2023

In this paper, we implement integrated magnetic flux concentrators
(MFCs) combined with a multi-frequency modulation method to achieve
high-magnetic-detection sensitivity using a nuclear spin on the solid
nuclear spin in diamonds. First, we excited the nuclear spin in
diamonds using a continuous-wave technique, and a linewidth of
1.37 MHz and frequency resolution of 79 Hz were
successfully obtained, which is reduced by one order of the linewidth,
and increased by 56 times in frequency resolution compared to that
excited by an electron spin. The integrated high-permeability MFC was
designed to magnify the magnetic field near the diamond, with a
magnification of 9.63 times. Then, the multi-frequency modulation
technique was used to fully excite the hyperfine energy level of
Nitrogen Vacancy (NV) centers along the four axes on the diamond with
MFC, and magnetic detection sensitivity of 250pT/Hz1/2 was realized. These techniques should
allow designing an integrated NV magnetometer with high sensitivity in
a small volume.
Importance of crystal length on the stability of a picosecond supercontinuum generated in undoped YAG
Authors: Roškot Lukáš , Novák Ondřej , Csanaková Bianka , Mužík Jiří , Smrž Martin , Jelínek Michal , Mocek Tomáš 
Published: April 2023

In this paper, we present the stability characteristics of a supercontinuum (SC) generated in a 60 mm, 100 mm, and 130 mm long undoped yttrium aluminum garnet (YAG) crystal pumped by a Yb thin-disk regenerative amplifier. The 1.2 ps pulses with a central wavelength of 1030 nm and repetition rate of 89 kHz are loosely focused (NA = 0.0031) into the crystal in order to prevent laser-induced damage and to increase the energy stability of the generated SC. Out of the tested crystal lengths, the optimal is experimentally determined to be 130 mm. The energy contained in the blueshifted side, which has a 530 nm spectral cutoff, is 71 nJ and exhibits average power stability of 0.4% RMS, which is 2.5× better than the stability of the pump beam.
Evaluation of the sugar-sweetened beverage tax in Oakland, United States, 2015–2019: A quasi-experimental and cost-effectiveness study
Authors: White Justin S. , Basu Sanjay , Kaplan Scott , Madsen Kristine A. , Villas-Boas Sofia B. , Schillinger Dean 
Published: April 2023

Background
While a 2021 federal commission recommended that the United States government levy a sugar-sweetened beverage (SSB) tax to improve diabetes prevention and control efforts, evidence is limited regarding the longer-term impacts of SSB taxes on SSB purchases, health outcomes, costs, and cost-effectiveness. This study estimates the impact and cost-effectiveness of an SSB tax levied in Oakland, California.

Methods and findings
An SSB tax ($0.01/oz) was implemented on July 1, 2017, in Oakland. The main sample of sales data included 11,627 beverage products, 316 stores, and 172,985,767 product-store-month observations. The main analysis, a longitudinal quasi-experimental difference-in-differences approach, compared changes in beverage purchases at stores in Oakland versus Richmond, California (a nontaxed comparator in the same market area) before and 30 months after tax implementation (through December 31, 2019). Additional estimates used synthetic control methods with comparator stores in Los Angeles, California. Estimates were inputted into a closed-cohort microsimulation model to estimate quality-adjusted life years (QALYs) and societal costs (in Oakland) from 6 SSB-associated disease outcomes. In the main analysis, SSB purchases declined by 26.8% (95% CI −39.0 to −14.7, p < 0.001) in Oakland after tax implementation, compared with Richmond. There were no detectable changes in purchases of untaxed beverages or sweet snacks or purchases in border areas surrounding cities. In the synthetic control analysis, declines in SSB purchases were similar to the main analysis (−22.4%, 95% CI −41.7% to −3.0%, p = 0.04). The estimated changes in SSB purchases, when translated into declines in consumption, would be expected to accrue QALYs (94 per 10,000 residents) and significant societal cost savings (>$100,000 per 10,000 residents) over 10 years, with greater gains over a lifetime horizon. Study limitations include a lack of SSB consumption data and use of sales data primarily from chain stores.

Conclusions
An SSB tax levied in Oakland was associated with a substantial decline in volume of SSBs purchased, an association that was sustained more than 2 years after tax implementation. Our study suggests that SSB taxes are effective policy instruments for improving health and generating significant cost savings for society.
Identifying optimal first-line immune checkpoint inhibitors based regiments for advanced non-small cell lung cancer without oncogenic driver mutations: A systematic review and network meta-analysis
Authors: Liu Tingting , Wu Sijia , Fang Weiwei , Li Hongkai , Su Lili , Qi Guiye , Li Huaichen , Liu Yao 
Published: April 2023

Background
Immune checkpoint inhibitors (ICIs) have changed the treatment pattern of advanced and metastatic NSCLC. A series of ICI based therapies have emerged in the first-line treatment field, but the comparative efficacy was unclear.

Method
We searched multiple databases and abstracts of major conference proceedings up to Apri1, 2022 for phase III randomised trials of advanced driver-gene wild type NSCLC patients receiving first-line therapy. Outcomes analyzed included progression free survival (PFS), overall survival (OS), and et al.

Results
Thirty-two double-blind RCTs were included, involving 18,656 patients assigned to 22 ICI-based first-line regimens. A series of ICI regiments (including ICI plus chemotherapy), ICI monotherapy, doublet ICIs, doublet ICIs plus chemotherapy) emerged, and showed significant PFS and OS benefit than chemotherapy and chemotherapy + bevacizumab (BEV) for advanced wild-type NSCLC. In comprehensive terms of PFS, chemoimmunotherapy (CIT) were significantly more effective than ICI monotherapy and doublet ICIs. In terms of OS for patients with non-squamous NSCLC, pembrolizumab containing CIT was associated with a median rank of the best regimens, and followed by Atezolizumab+BEV based CIT; while for OS in patients with squamous NSCLC, Cemiplimab and sintilimab based CIT were the most effective regimens. For more than 2 years follow-up, the atezolizumab, pembrolizumab, nivolumab and durvalumab containing ICI therapy all provide a durable long-term OS benefit over chemotherapy and BEV + chemotherapy.

Conclusions
The findings of the present NMA represent the most comprehensive evidence, which might suggest or provide basis for first-line ICI therapy decision for advanced NSCLC patients without oncogenic driver mutations.
Access to perinatal healthcare in minority Anglophones: Hospital type and birth outcomes
Authors: Auger Nathalie , Bilodeau-Bertrand Marianne , Lafleur Nahantara 
Published: April 2023

Objectives
We assessed the relationship between hospital characteristics and risk of adverse birth outcomes among minority Anglophones in Montreal, Canada.

Methods
The study included 124,670 births among Anglophones in metropolitan Montreal between 1998 and 2019. We estimated risk ratios (RR) and 95% confidence intervals (CI) for the association between hospital characteristics, including residential proximity to hospitals and language in which medical services are provided, and risks of preterm birth and stillbirth. Models were adjusted for maternal socioeconomic status and other characteristics.

Results
In this study, 8% of Anglophones had a preterm birth and 0.4% a stillbirth. Anglophone women who delivered at a farther French hospital had a greater risk of stillbirth (RR 1.67, 95% CI 1.28–2.18) than preterm birth (RR 1.21, 95% CI 1.14–1.30), compared with delivery at hospitals closer to home. In contrast, delivery at a farther English hospital was associated with similar risks of stillbirth (RR 1.36, 95% CI 1.08–1.71) and preterm birth (RR 1.36, 95% CI 1.29–1.44). The greater risk of stillbirth with delivery at a farther French hospital, versus greater risk of preterm birth at a farther English hospital, remained present in analyses stratified by maternal age, education, material deprivation, and region of origin.

Conclusion
Minority Anglophones in Montreal who travel to a farther French hospital for delivery have a greater risk of stillbirth than Anglophones who travel to a farther English hospital. This novel observation suggests the need to determine if access to perinatal healthcare in a woman’s language may help reduce the risk of stillbirth.
Ethics of Chemistry
Authors: Mahaffy Peter 
Published: April 2023

Abstract
Imagine yourself a chemist confronted with the use of chemical weapons during World War IO. Or a citizen living in Bhopal, India, perhaps having negotiated the siting of the plant in your city as a politician or an employee bringing home a good wage working at Union Carbide’s methyl isocyanate plant. Or perhaps you are a student in 2023 needing to make informed choices about climate engineering or the rapid full-scale extraction of rare-earth materials to mitigate the climate crisis. What is the right thing to do in each of these scenarios? How can the study of the moral values, norms, judgments, and virtues relevant to chemistry equip students to deeply reflect about the practice of chemistry and guide them to make informed and responsible choices about their behaviour as professionals?
Why is Presentism Intuitive?
Authors: Graziani Ernesto 
Published: April 2023

AbstractPresentism is, roughly, the ontological view that only the present exists. Among the philosophers engaged in the metaphysics of time there is wide agreement that presentism is intuitive (or commonsensical) and that its intuitiveness counts as evidence in its favour. My contribution has two purposes: first, defending the view that presentism is intuitive from some recent criticisms; second, putting forth a genealogical (or debunking) argument aimed at depriving presentism’s intuitiveness of the evidential value commonly granted to it.
Crystal structure of 2-methoxy-4-(methoxy-carbonyl)phenyl 2-chloro-4-fluorobenzoate, C16H12ClFO5
Authors: Xiong Xin-Ting , Xiao Pan-Lei , Ou-Yang Xiao-Hui , Nie Xu-Liang , Song Xiu-Ying 
Published: April 2023

Abstract
C16H12ClFO5, monclinic, P21/c (no. 14), a = 14.7536(6) Å, b = 7.8281(3) Å, c = 14.0501(7) Å, β =

111.174

(
5
)

°

$111.174(5){}^{\circ}$

, V = 1513.13(12) Å3, Z = 4, R

gt
(F) = 0.0367, wR

ref
(F
2) = 0.0865, T = 296(2) K.
Re-examination of nonlinear vibration and nonlinear bending of porous sandwich cylindrical panels reinforced by graphene platelets
Authors: Shen Hui-Shen , Li Chong 
Published: April 2023

Abstract
This article re-examines the nonlinear vibration and nonlinear bending responses of porous sandwich cylindrical panels reinforced by graphene platelets resting on elastic foundations in thermal environments. The graphene platelet-reinforced composite (GPLRC) core is assumed to be of multilayers, and each layer may have different porosity coefficient values to achieve a piece-wise functionally graded pattern. By introducing an inhomogeneous model instead of the equivalent isotropic model (EIM), the Young’s moduli along with the shear modulus of the porous GPLRC core are predicted through a generic Halpin–Tsai model in which the porosity is included. The thermomechanical properties of metal face sheets and the porous GPLRC core are assumed to be temperature-dependent. Governing equations of motion for sandwich cylindrical panels with porous GPLRC core are formulated based on Reddy’s third-order shear deformation theory coupled with von Kármán nonlinear strain–displacement relationships. In the modeling, the panel–foundation interaction and the thermal effects are also considered. The analytical solutions for the nonlinear vibration and nonlinear bending problems are obtained by applying a two-step perturbation approach. Numerical studies are performed to compare the results obtained from the present model and the EIM. The results confirm that the EIM is not suitable for linear free vibration analysis of sandwich cylindrical panels with the porous GPLRC core, but the EIM may be valid for the cases of nonlinear vibration and nonlinear bending analyses of the same panel resting on Pasternak elastic foundations.
Plant based fabrication of CuO/NiO nanocomposite: a green approach for low-level quantification of vanillin in food samples
Authors: Shah Shuaibul-Zaman , Taqvi Iqleem H. , Ameen Sidra , Mallah Arfana , Buledi Jamil A. , Khand Nadir H. , Solangi Amber R. 
Published: April 2023

Abstract
Metal oxide nanoparticles have a wide range of potential applications due to their unique properties, including their high surface area-to-volume ratio, enhanced reactivity, and unique electronic and magnetic properties. They are widely used in industries such as electronics, energy, catalysis, and medicine, where their small size and unique properties can be utilized for a variety of purposes. Fabrication of bi-metal oxide nanocomposite through green synthesis routes is of great importance. The plant leaves are being used as a prominent source for reducing/capping or functionalization of metal oxide nanoparticles. Thus, engineering of CuO/NiO nanocomposite has been carried out via exploiting Java plum leaves’ extract as reducing and capping source. After successful green synthesis, the CuO/NiO nanocomposite was directed for characterization through advanced analytical tool viz XRD, FTIR and TEM. The FTIR spectra confirm the stretching frequencies of Cu–O and Ni–O bonds at 587.1 and 647.3 cm−1 which witnesses the surface functionalities of prepared nanocomposite. XRD analysis confirms the diffraction patterns of CuO/NiO nanocomposite with exceptional crystalline structure and phase purity. TEM images evaluate the nanometric size of fabricated CuO/NiO nanocomposite with nanoflower like internal texture having size below 100 nm. The as-prepared CuO/NiO nanocomposite was used as green electrocatalyst for the determination of vanillin (VN) in food samples. The redox current response and charge transfer kinetics of CuO/NiO/GCE was examined via cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimal conditions such as potential window (0.2–1.3 V), PBS electrolyte pH 7, scan rate 60 mV/s, the engineered modified electrode exhibited excellent response for VN. The LOD of prepared electrocatalyst for VN was examined as 0.0087 µM. The potential of applicability of CuO/NiO/GCE was tested in ice-cream and chocolate samples. The obtained recovery values of real samples were found in acceptable range which highlights analytical potential of prepared modified electrode for VN.