bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term outcomes of reduced-port laparoscopic surgery versus conventional laparoscopic surgery for total gastrectomy: a single-institute experience
Authors: Teng Wenhao , Liu Jingfu , Liu Wenju , Jiang Jianping , Chen Meimei , Zang Weidong 
Published: March 2023

Abstract
Background
The efficacy of reduced-port laparoscopic surgery (RLS) for total gastrectomy remains unclear. This study focused on evaluating the short-term outcomes of RLS compared with conventional laparoscopic surgery (CLS) for total gastrectomy.

Methods
One hundred and ten patients who underwent completed laparoscopic total gastrectomy for gastric cancer between September 2018 and June 2022 were retrospectively collected and classified into two groups (65 CLS and 45 RLS) according to different operation approach. Twenty-four RLS cases underwent single-incision plus two ports laparoscopic surgery (SILS + 2) and twenty-one underwent single-incision plus one port laparoscopic surgery (SILS + 1). Surgical outcomes, pain intensity, cosmetic and postoperative morbidity, and mortality were compared between groups.

Results
The overall incidence of postoperative complications was similar between the CLS group and the RLS group (16.9% vs. 8.9%, P = 0.270). It was also comparable in the Clavien-Dindo classification (P = 0.774). However, compared with the CLS group, the RLS group had a significantly shorter total length of incision (5.6 ± 1.0 cm vs. 7.1 ± 0.7 cm, P = 0.000); shorter time to first ambulation (24.9 ± 5.9 h vs. 27.6 ± 5.0 h, P = 0.009), flatus (3.0 ± 0.8 d vs. 3.5 ± 1.0 d, P = 0.022) and oral intake (4.0 ± 1.6 d vs. 6.1 ± 5.1 d, P = 0.011); lower white blood cell count on the third day after the operation (9.8 ± 4.0*109/L vs. 11.6 ± 4.7*109/L, P = 0.037); and lower visual analogue scale score on postoperative days 1 and 3(3.0 ± 0.7 vs. 3.3 ± 0.7, P = 0.044 and 0.6 ± 0.7 vs. 1.6 ± 0.6, P = 0.000 respectively). On the other hand, it didn’t find any difference in short-term outcomes between the SILS + 2 group and the SILS + 1 group (P > 0.05). But the proximal resection margin was longer in the SILS + 2 group than in the SILS + 1 group (2.6 ± 0.7 cm vs. 1.5 ± 0.9 cm, P = 0.046) in patients with adenocarcinoma of the esophagogastric junction (AEG).

Conclusions
RLS for total gastrectomy is a feasible and safe technique when performed by an experienced laparoscopic surgeon. Moreover, compared with SILS + 1, SILS + 2 might have some advantages in AEG patients.
Design and implementation characteristics of research training for rural health professionals: a qualitative descriptive study
Authors: Quilliam Claire , Wong Shee Anna , Corboy Denise , Glenister Kristen , King Olivia , Mc Namara Kevin , Alston Laura , Aras Drew , Beauchamp Alison , McKinstry Carol 
Published: March 2023

Abstract
Background
Research capacity and capability of rural health professionals is essential to the delivery of evidence-based care and for informing strategies to address rural health inequities. Effective implementation of research education and training is fundamental to building rural health professional research capacity and capability. A lack of overarching guidance to inform the delivery of research education and training in rural health services can contribute to gaps in capacity-building approaches. The aim of this study was to identify characteristics of the design and implementation of current research training for rural health professionals in Victoria, Australia, to inform a future model for rural health professional research capacity and capability building.

Methods
A qualitative descriptive study was undertaken. Key informants, with extensive knowledge of research education and training in rural health services in Victoria, were invited to participate in semi-structured telephone interviews via snowballing recruitment methods. Interview transcripts were analysed inductively, with themes and codes mapped to the domains of the Consolidated Framework for Implementation Research.

Results
Of the 40 key informants approached, 20 agreed to participate including 11 regional health service managers, five rural health academics and four university managers. Participants suggested that research training varied in quality and relevance to rural health professionals. Training costs and lack of tailoring to the rural context were key barriers, whereas experiential learning and flexible modes of delivery enabled training uptake. Health service and government policies, structures, and processes both enabled or stifled implementation opportunities, with rural health professional networks from different regions offering capacity for research training development, and government departmental structures hampering training coordination. Tension between research activities and clinical practice, and health professional knowledge and beliefs, shaped the delivery of training programs. Strategically planned and evaluated research training programs and education via co-design with rural health professionals and use of research champions were strongly recommended by participants.

Conclusions
To optimise research training for rural health professionals and increase the quality and quantity of relevant rural health research, a systematically planned, implemented, and resourced region-wide research training model is required.
Generational effect on nurses’ work values, engagement, and satisfaction in an acute hospital
Authors: Tan Si Hui Evangeline , Chin Guey Fong 
Published: March 2023

Abstract
Background
The present nursing workforce comprises four generational of nurses working side–by–side. While such a generation blend adds invaluable diversity to the workforce, it also brings added complexity. The study aimed to describe and summarise work values and attitudes of four nursing generations, namely Baby boomers, Generation X, Y and Z.

Method
A cross-sectional questionnaire study was adopted. A total of 778 nurses from an acute hospital in Singapore completed the online questionnaire. The Work Value and Attitude scale measuring seven constructs (Work Centrality, Non-compliance, Technology Challenge, Work life balance, leadership, Power, and Recognition) was employed for data collection.

Results
The Cronbach’s alpha was 0.714 for the overall instrument. Statistically significant differences amongst the four generations of nurses emerged in the Work Value and Attitude scale in the construct of non-compliance (p = 0.007), technology challenge (p = 0.027), work-life balance (p < 0.001), and recognition (p < 0.001). No statistically significant differences were noted for the rest of the constructs.

Discussion and conclusion
The findings of this study highlight that differences in work values and attitudes exist among nurses of different generations. Generation X are less likely to challenge the conventional norm and supervisors. Generation Y and Z are the most tech-savvy generations and can adapt quickly to new technology. There is also a greater emphasis on work-life balance as the generation gets younger. Generation Y and Z nurses perceived that younger nurses do not get due respect and recognition from their colleagues. Acknowledging the generational differences in work values and attitudes can facilitate nursing management to tailor strategies to improve individual and organisation performance while creating a work environment that enhances intergeneration harmony and teamwork.
Prevention of invasive ventilation (PRiVENT)—a prospective, mixed-methods interventional, multicentre study with a parallel comparison group: study protocol
Authors: Michels Julia D. , Meis Jan , Sturm Noemi , Bornitz Florian , von Schumann Selina , Weis Aline , Neetz Benjamin , Bentner Martina , Forstner Johanna , Litke Nicola , Wensing Michel , Erdmann Stella , Grobe Thomas , Frerk Timm , Kempa Axel , Neurohr Claus , Schneider Armin , Müller Michael , Herth Felix J. F. , Szecsenyi Joachim , Trudzinski Franziska C. ,   the PRiVENT-Study Group, Trudzinski Franziska Christina , Iberl Gabriele , Müller Beatrice , Biehler Elena , Fleischhhauer Thomas , Fuchs Gerhard , Qreini Markus , Schubert-Haack Janina , Klingenberg Anja , Kempa Alex , Joves Biljana , Rheinhold Andreas , Ghiani Alessandro , Lutz Nina , Walcher Swenja , Tsitouras Konstantinos , Paderewska Joanna , Briese Selina , Andritschky Christoph , Gehrig Patrick , Sugg Joachim , Hirschmann Susanne , Britsch Simone , Straub Christa , Jabbour Claude , Hahn Michael , Krebs Jörg , Graf Peter-Tobias , Denzer Petra , Fiedler Mascha O. , Bomeken Miriane , Stier Sebastian , Terboven Tom , Merle Uta , Regula Jens , Müller Jens , Oltmanns Ute , Hennersdorf Marcus , Satir Neslihan , Borst Mathias , Mayer Brigitte , Reikow Wolfgang , Kredel Markus , Frey Konstantin , Wolff Holger , Seidlitz Florian , Bientzle Stefanie , Nohé Boris , Allgäuer Sebastian , Schöpp Alexej , Schlegel Christoph , Hübner Imke , Kuzniar Andrezj , Häberle Helene , Riessen Reimer , Schempf Benjamin , Rebenschütz Ingo , Straub Andreas , Kollum Marc , Winter Markus , Hartveg Paul , Junginger Andreas , Beck Helmut , Vogel Mathias 
Published: March 2023

Abstract
Background

Invasive mechanical ventilation (IMV) is a standard therapy for intensive care patients with respiratory failure. With increasing population age and multimorbidity, the number of patients who cannot be weaned from IMV increases, resulting in impaired quality of life and high costs. In addition, human resources are tied up in the care of these patients.

Methods
The PRiVENT intervention is a prospective, mixed-methods interventional, multicentre study with a parallel comparison group selected from insurance claims data of the health insurer Allgemeine Ortskrankenkasse Baden-Württemberg (AOK-BW) conducted in Baden-Württemberg, Germany, over 24 months. Four weaning centres supervise 40 intensive care units (ICUs), that are responsible for patient recruitment. The primary outcome, successful weaning from IMV, will be evaluated using a mixed logistic regression model. Secondary outcomes will be evaluated using mixed regression models.

Discussion
The overall objective of the PRiVENT project is the evaluation of strategies to prevent long-term IMV. Additional objectives aim to improve weaning expertise in and cooperation with the adjacent Intensive Care Units.

Trial registration
This study is registered at ClinicalTrials.gov (NCT05260853).
Mesenchymal stromal cells modulate infection and inflammation in the uterus and mammary gland
Authors: Schouten Iftach , Bernys-Karolys Andrés , Schneider Peleg , Dror Tal , Ofer Lior , Shimoni Chen , Nissim-Eliraz Einat , Shpigel Nahum Y. , Schlesinger Sharon 
Published: March 2023

Abstract
The use of mesenchymal stromal cells (MSCs) is emerging as an efficacious and safe treatment for many infectious and non-infectious inflammatory diseases in human and veterinary medicine. Such use could be done to treat mastitis and metritis, which are the most common disease conditions affecting dairy cows leading to considerable economic losses and reduced animal welfare. Currently, both disease conditions are commonly treated using local and systemic administration of antibiotics. However, this strategy has many disadvantages including low cure rates and the public health hazards. Looking for alternative approaches, we investigated the properties of MSCs using in-vitro mammary and endometrial cell systems and in-vivo mastitis and metritis murine model systems. In-vitro, co-culture of mammary and uterus epithelial cells constructed with NF-kB reporter system, the master regulator of inflammation, demonstrated their anti-inflammatory effects in response to.LPS. In vivo, we challenge animals with field strains of mammary and utero pathogenic Escherichia coli and evaluated the effects of local and systemic application of MSC in the animal models. Disease outcome was evaluated using histological analysis, bacterial counts and gene expression of inflammatory markers. We show that MSC treatment reduced bacterial load in metritis and significantly modulated the inflammatory response of the uterus and mammary gland to bacterial infection. Most notably are the immune modulatory effects of remotely engrafted intravenous MSCs, which open new avenues to the development of MSC-based cell-free therapies.
AVPR2 is a potential prognostic biomarker and correlated with immune infiltration in head and neck squamous cell carcinoma
Authors: Mao Linwei , Pan Zhiyong , Chen Wenzhi , Hu Weiqun , Chen Xiufen , Dai Huiting 
Published: March 2023

Abstract
Purpose
To explore the potential of AVPR2 in the immunotherapy of head and neck squamous cell carcinoma (HNSCC), thus providing insights into a novel antitumour strategy.

Methods
In this study, we performed a comprehensive analysis of the AVPR2 gene in HNSCC using public datasets from The Cancer Genome Atlas and Gene Expression Omnibus. We explored the potential molecular mechanism of HNSCC in clinical prognosis and tumour immunity from the aspects of gene expression, prognosis, immune subtypes, and immune infiltration.

Results
AVPR2 expression was significantly downregulated in primary HNSCC tissue compared with normal tissue. HNSCC patients with high AVPR2 expression had a better prognosis. Moreover, the results of GSEA showed that immune subtype surface AVPR2 is involved in immune modulation. Furthermore, significant strong correlations between AVPR2 expression and infiltrating immune cells existed in HNSCC, and marker genes of infiltrating immune cells were also significantly related to AVPR2 expression in HNSCC. These results suggest that AVPR2 expression can influence the infiltration of tumour immune cells. Finally, we found that only high levels of B-cell infiltration, rather than those of other immune cells, can predict a longer overall survival in patients with HNSCC. Future studies are needed to explore the role of AVPR2 and tumour-infiltrating B cells in HNSCC.

Conclusions
The AVPR2 gene may be a prognostic biomarker of HNSCC. Moreover, AVPR2 may play a role in HNSCC immune modulation, and the regulation of tumour-infiltrating B cells by AVPR2 may be a key link.
Barriers to cancer treatment and care for people experiencing structural vulnerability: a secondary analysis of ethnographic data
Authors: Bourgeois Amber , Horrill Tara C. , Mollison Ashley , Lambert Leah K. , Stajduhar Kelli I. 
Published: March 2023

Abstract
Background
A key pillar of Canada’s healthcare system is universal access, yet significant barriers to cancer services remain for people impacted by structural vulnerability (e.g., poverty, homelessness, racism). For this reason, cancer is diagnosed at a later stage, resulting in worse patient outcomes, a reduced quality of life, and at a higher cost to the healthcare system. Those who face significant barriers to access are under-represented in cancer control services Consequently, these inequities result in people dying from cancers that are highly treatable and preventable, however; little is known about their treatment and care course. The aim of this study was to explore barriers to accessing cancer treatment among people experiencing structural vulnerability within a Canadian context.

Methods
We conducted a secondary analysis of ethnographic data informed by critical theoretical perspectives of equity and social justice. The original research draws from 30 months of repeated interviews (n = 147) and 300 h of observational fieldwork with people experiencing health and social inequities at the end-of-life, their support persons, and service providers.

Results
Our analysis identified four themes presenting as ‘modifiable’ barriers to inequitable access to cancer treatment: (1) housing as a key determinant for cancer treatment (2) impact of lower health literacy (3) addressing social care needs is a pre-requisite for treatment (4) intersecting and compounding barriers reinforce exclusion from cancer care. These inter-related themes point to how people impacted by health and social inequities are at times ‘dropped’ out of the cancer system and therefore unable to access cancer treatment.

Conclusion
Findings make visible the contextual and structural factors contributing to inequitable access to cancer treatment within a publically funded healthcare system. Identifying people who experience structural vulnerability, and approaches to delivering cancer services that are explicitly equity-oriented are urgently needed.
SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Authors: Korneeva Nadejda , Khalil Md Imtiaz , Ghosh Ishita , Fan Ruping , Arnold Thomas , De Benedetti Arrigo 
Published: March 2023

AbstractWhen viruses like SARS-CoV-2 infect cells, they reprogram the repertoire of cellular and viral transcripts that are being translated to optimize their strategy of replication, often targeting host translation initiation factors, particularly eIF4F complex consisting of eIF4E, eIF4G and eIF4A. A proteomic analysis of SARS-CoV-2/human proteins interaction revealed viral Nsp2 and initiation factor eIF4E2, but a role of Nsp2 in regulating translation is still controversial. HEK293T cells stably expressing Nsp2 were tested for protein synthesis rates of synthetic and endogenous mRNAs known to be translated via cap- or IRES-dependent mechanism under normal and hypoxic conditions. Both cap- and IRES-dependent translation were increased in Nsp2-expressing cells under normal and hypoxic conditions, especially mRNAs that require high levels of eIF4F. This could be exploited by the virus to maintain high translation rates of both viral and cellular proteins, particularly in hypoxic conditions as may arise in SARS-CoV-2 patients with poor lung functioning.
Cotton plant part 3D segmentation and architectural trait extraction using point voxel convolutional neural networks
Authors: Saeed Farah , Sun Shangpeng , Rodriguez-Sanchez Javier , Snider John , Liu Tianming , Li Changying 
Published: March 2023

Abstract
Background
Plant architecture can influence crop yield and quality. Manual extraction of architectural traits is, however, time-consuming, tedious, and error prone. The trait estimation from 3D data addresses occlusion issues with the availability of depth information while deep learning approaches enable learning features without manual design. The goal of this study was to develop a data processing workflow by leveraging 3D deep learning models and a novel 3D data annotation tool to segment cotton plant parts and derive important architectural traits.

Results
The Point Voxel Convolutional Neural Network (PVCNN) combining both point- and voxel-based representations of 3D data shows less time consumption and better segmentation performance than point-based networks. Results indicate that the best mIoU (89.12%) and accuracy (96.19%) with average inference time of 0.88 s were achieved through PVCNN, compared to Pointnet and Pointnet++. On the seven derived architectural traits from segmented parts, an R2 value of more than 0.8 and mean absolute percentage error of less than 10% were attained.

Conclusion
This plant part segmentation method based on 3D deep learning enables effective and efficient architectural trait measurement from point clouds, which could be useful to advance plant breeding programs and characterization of in-season developmental traits. The plant part segmentation code is available at https://github.com/UGA-BSAIL/plant_3d_deep_learning.
A modified hybrid transport technique combined with a retrograde tibiotalocalcaneal arthrodesis nail for the management of distal tibial periarticular osteomyelitis and associated defects
Authors: Wang ChaoFeng , Ma Teng , Li Zhao , Wang Qian , Li Zhong , Zhang Kun , Huang Qiang 
Published: March 2023

Abstract
Background
This paper aimed to propose a modified technique of bone transport. An annular frame combined with a retrograde tibiotalocalcaneal arthrodesis nail was used in this novel technique for treating large distal tibial periarticular osteomyelitis and associated defects.

Methods
Our team conducted a retrospective research. Forty-three patients with large distal tibial periarticular bone loss were involved in this study. Sixteen patients were treated using the modified hybrid transport technique (MHT group) while 27 were subjected to traditional bone transport (BT group). The mean bone loss was 7.8 ± 2.4 cm in the MHT group and 7.6 ± 2.6 cm in the BT group. The external fixation index, time in transport frame, self-rating anxiety scale, bone healing results and postoperative complications were recorded.

Results
The mean time in frame for the MHT group was 3.6 ± 1.5 months, while that of the BT group was 10.3 ± 2.7 months (p < 0.05). The mean external fixation index of MHT group was 0.46 ± 0.08 months/cm versus 1.38 ± 0.24 months/cm of the BT group (p < 0.05). There was no statistical difference for the bone healing results between the MHT and BT groups (p = 0.856). The self-rating anxiety scale and total complication incidence of the MHT group were significantly lower than that of BT patients (p < 0.05).

Conclusion
Compared to the traditional BT technique, our modified hybrid transport technique showed better clinical outcomes for treating large distal tibial periarticular bone loss, including less time in transport frame, lower external fixation index and complication incidence. Therefore, this modified technique should be further promoted and developed.
Percutaneous mesh-container-plasty versus percutaneous kyphoplasty for the treatment of Kümmell's disease: a retrospective cohort study
Authors: Li Yimin , Qian Yunfan , Shen Guangjie , Tang Chengxuan , Zhong Xiqiang , He Shaoqi 
Published: March 2023

Abstract
Background
Both percutaneous kyphoplasty (PKP) and percutaneous mesh-container-plasty (PMCP) were important procedures for the treatment of Kümmell's disease. This study aimed to compare the clinical and radiological results of PKP and PMCP for the treatment of Kümmell's disease.

Methods
This study included patients with Kümmell's disease treated at our center between January 2016 and December 2019. A total of 256 patients were divided into two groups according to the surgical treatment they received. Clinical, radiological, epidemiological, and surgical data were compared between the two groups. Cement leakage, height restoration, deformity correction, and distribution were evaluated. The visual analog scale (VAS), Oswestry Disability Index (ODI), and short-form 36 health survey domains “role-physical” (SF-36 rp) and “bodily pain” (SF-36 bp) were calculated preoperatively, immediately after surgery, and 1-year postoperatively.

Results
The VAS and ODI scores improved in the PKP [preoperative: 6 (6–7), 68.75 ± 6.64; postoperative: 2 (2–3), 23.25 ± 3.50, respectively] (p < 0.05) and the PMCP [preoperative: 6 (5–7), 67.70 ± 6.50; postoperative: 2 (2–2), 22.24 ± 3.55, respectively] groups (p < 0.05). There were significant differences between the two groups. The mean cost in the PKP group was lower than that in the PMCP group (3697 ± 461 vs. 5255 ± 262 USD, p < 0.05). The cement distribution in the PMCP group was significantly higher than that in the PKP group (41.81 ± 8.82% vs. 33.65 ± 9.24%, p < 0.001). Cement leakage was lower in the PMCP group (23/134) than in the PKP group (35/122) (p < 0.05). The anterior vertebral body height ratio (AVBHr) and Cobb’s angle improved in the PKP (preoperative: 70.85 ± 16.62% and 17.29 ± 9.78°; postoperative: 80.28 ± 13.02% and 13.05 ± 8.40°, respectively) and PMCP (preoperative: 70.96 ± 18.01% and 17.01 ± 10.53°; postoperative: 84.81 ± 12.96% and 10.76 ± 9.23°, respectively) groups (p < 0.05). There were significant differences in vertebral body height recovery and segmental kyphosis improvement between the two groups.

Conclusions
PMCP had advantages over PKP in terms of pain relief and functional recovery for the treatment of Kümmell's disease. Moreover, PMCP is more effective than PKP in preventing cement leakage, increasing cement distribution, and improving vertebral height and segmental kyphosis, despite its higher cost.