bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycemic outcomes of a family-focused intervention for adults with type 2 diabetes: Main, mediated, and subgroup effects from the FAMS 2.0 RCT
Authors: Nelson Lyndsay A. , Spieker Andrew J. , Greevy Robert A. , Roddy McKenzie K. , LeStourgeon Lauren M. , Bergner Erin M. , El-Rifai Merna , Aikens James E. , Wolever Ruth Q. , Elasy Tom A. , Mayberry Lindsay S. 
Published: September 2023

AbstractAimsFamily/friends Activation to Motivate Self-care (FAMS) is a self-care support intervention delivered via mobile phones. We evaluated FAMS effects on hemoglobin A1c (HbA1c) and intervention targets among adults with type 2 diabetes in a 15-month RCT.MethodsPersons with diabetes (PWDs) and their support persons (family/friend, optional) were randomized to FAMS or control. FAMS included monthly phone coaching and text messages for PWDs, and text messages for support persons over a 9-month intervention period.ResultsPWDs (N=329) were 52% male, 39% from minoritized racial or ethnic groups, with mean HbA1c 8.6±1.7%. FAMS improved HbA1c among PWDs with a non-cohabitating support person (−0.64%; 95% CI [-1.22%, −0.05%]), but overall effects were not significant. FAMS improved intervention targets including self-efficacy, dietary behavior, and family/friend involvement during the intervention period; these improvements mediated post-intervention HbA1c improvements (total indirect effect −0.27%; 95% CI [-0.49%, −0.09%]) and sustained HbA1c improvements at 12 months (total indirect effect −0.19%; 95% CI [−0.40%, −0.01%]).ConclusionsDespite improvements in most intervention targets, HbA1c improved only among PWDs engaging non-cohabitating support persons suggesting future family interventions should emphasize inclusion of these relationships. Future work should also seek to identify intervention targets that mediate improvements in HbA1c.
Well-being outcomes of a family-focused intervention for persons with type 2 diabetes and support persons: Main, mediated, and subgroup effects from the FAMS 2.0 RCT
Authors: Roddy McKenzie K. , Spieker Andrew J. , Nelson Lyndsay A , Greevy Robert A. , LeStourgeon Lauren M. , Bergner Erin M. , El-Rifai Merna , Elasy Tom A. , Aikens James E. , Wolever Ruth Q. , Mayberry Lindsay S. 
Published: September 2023

AbstractAimsType 2 diabetes self-management occurs within social contexts. We sought to test the effects of Family/friends Activation to Motivate Self-care (FAMS), a self-care support intervention delivered via mobile phones, on psychosocial outcomes for persons with diabetes (PWDs) and their support persons.MethodsPWDs had the option to enroll with a friend/family member as a support person in a 15-month RCT to evaluate FAMS versus enhanced usual care. FAMS included 9-months of monthly phone coaching and text message support for PWDs, and text message support for enrolled support persons.ResultsPWDs (N=329) were 52% male and 39% from minoritized racial or ethnic groups; 50% enrolled with elevated diabetes distress. Support persons (N=294) were 26% male and 33% minoritized racial or ethnic groups. FAMS improved PWDs’ diabetes distress (d=-0.19) and global well-being (d=0.21) during the intervention, with patterns of larger effects among minoritized groups. Post-intervention and sustained (15-month) improvements were driven by changes in PWDs’ self-efficacy, self-care behaviors, and autonomy support. Among support persons, FAMS improved helpful involvement without increasing burden or harmful involvement.ConclusionsFAMS improved PWDs’ psychosocial well-being, with post-intervention and sustained improvements driven by improved self-efficacy, self-care, and autonomy support. Support persons increased helpful involvement without adverse effects.
Incidence of and Risk Factors for New-Onset Urinary and Fecal Incontinence After Acute Stroke
Authors: Cruz Enrique , Wells Yvonne , Miller Charne , Lannin Natasha A. , Cloud Geoffrey C. 
Published: September 2023

ABSTRACTBackground and PurposeUnderstanding the risk factors for incontinence after acute stroke allows for early identification and treatment of patients who are likely to experience this complication. This study estimates incidence of urinary and fecal incontinence (UI and FI) after stroke in adults and examines the association of risk factors.MethodsScreening data of a prospective study were analyzed. Included were 652 consecutive patients admitted to an acute stroke unit over a 15-month period beginning May 2021. Those with pre-existing UI or FI were excluded from the analysis. Bivariate analyses examined unadjusted relationships between both UI and FI and age, sex, stroke severity, premorbid disability, stroke type, and stroke anatomical site. Regression analyses were performed to build iterative models.ResultsA total of 418 patients had an acute stroke without pre-existing incontinence. The median age of stroke survivors was 76 years (IQR66-84), 58% were men, and 76% had an ischemic stroke. Within the first 7 days after stroke, 128 (29%) had UI and 115 (26%) had FI. Increasing age, premorbid disability, and stroke severity increased the risk of both new-onset UI and FI after stroke. Those with intraventricular hemorrhage were 2.9 times more likely to develop UI. Women were 1.7 times more likely to have UI after stroke than men. Intraventricular hemorrhage increased the risk of new-onset FI after stroke by 2.9 times, while lobar hemorrhage increased the risk by 2.2 times.ConclusionsUrinary and fecal incontinence continue to be relatively common after acute stroke, although incidence rates are lower than 20th century estimates. Patients are more likely to require incontinence assessment, monitoring and care if they are older, had higher premorbid disability, and have experienced more severe stroke or intraventricular hemorrhage. Provision of continence care and rehabilitation remain essential components of stroke services.
Left Ventricular Assist Device Outcomes and Strategy 5 Years from Heart Transplant Allocation Score Change
Authors: Agronin Jacob , Brown Meredith , Calvelli Hannah , Rakita Val , Toyoda Yoshiya , Kashem Mohammed Abul 
Published: September 2023

AbstractBackgroundThe United Network for Organ Sharing (UNOS) adopted new criteria for the heart allocation score on 10/18/2018 to reflect changing trends of candidates’ mortality while awaiting transplant. We examined the impact of these policy changes on rates of left ventricular assist device (LVAD) implantation and outcomes posttransplant from a relatively newer UNOS database.MethodsThe UNOS registry was used to identify first-time adult heart recipients with LVAD at listing or transplant who underwent transplantation between 1/1/2016 and 3/10/2020. Survival data was collected through 3/30/2023. Those listed prior to 10/18/2018 but transplanted after were excluded. Patients were divided into before or after change groups. Demographics and clinical parameters were compared. Survival was analyzed with Kaplan-Meier curves and log-rank tests. A p<0.05 was considered significant.ResultsWe identified 4599 heart recipients with LVAD in the before (N=3767) and after (N=832) score change eras. The after group had a lower rate of LVAD implantation while listed compared to the before group (19.4% vs 34.5%, p<0.0001), younger average age (53.1 ± 12.2 vs 54.1 ± 11.9, p=0.0350) and more likely to be female (24.9% vs 19.6%, p=0.0007); in both groups, most recipients (62%) were white. There was significantly farther distance from the donor hospital to transplant center in the after group (259.5 ± 246.8 NM vs 143.2 ± 182.1 NM, p<0.0001) and decreased waitlist days (83.5 ± 103.5 vs 369.0 ± 458.5, p<0.0001). Recipients in the after group were more likely to receive a CDC increased-risk donor organ (37.5% vs 30.2%, p=0.0002). Survival at 5-years was significantly reduced in the after group (60.5% vs 78.9%, p<0.0001).ConclusionsThe allocation score change in 2018 yielded considerable changes in mechanical circulatory support device implantation strategy and survival. The rate of LVAD implantation decreased with profoundly worse 5-year survival, showing further divergence from prior short-term post-transplant data.
miRNA and mRNA Signatures in Human Acute Kidney Injury Tissue
Authors: Janosevic Danielle , De Luca Thomas , Ferreira Ricardo Melo , Gisch Debora L. , Hato Takashi , Luo Jinghui , Yang Yingbao , Hodgin Jeffrey B. , Dagher Pierre C. , Eadon Michael T. 
Published: September 2023

AbstractAcute kidney injury (AKI) is an important contributor to the development of chronic kidney disease (CKD). There is a need to understand molecular mediators that drive either recovery or progression to CKD. In particular, the role of miRNA and its regulatory role in AKI is poorly understood. We performed miRNA and mRNA sequencing on biobanked human kidney tissues obtained in the routine clinical care of patients with the diagnoses of AKI and minimal change disease (MCD), in addition to nephrectomized (Ref) tissue from individuals without known kidney disease. Transcriptomic analysis of mRNA revealed that Ref tissues exhibited a similar injury signature to AKI, not identified in MCD samples. The transcriptomic signature of human AKI was enriched with genes in pathways involved in cell adhesion and epithelial-to-mesenchymal transition (e.g.,CDH6, ITGB6, CDKN1A). miRNA DE analysis revealed upregulation of miRNA associated with immune cell recruitment and inflammation (e.g., miR-146a, miR-155, miR-142, miR-122). These miRNA (i.e., miR-122, miR-146) are also associated with downregulation of mRNA such asDDR2andIGFBP6, respectively. These findings suggest integrated interactions between miRNAs and target mRNAs in AKI-related processes such as inflammation, immune cell activation and epithelial-to-mesenchymal transition. These data contribute several novel findings when describing the epigenetic regulation of AKI by miRNA, and also underscores the importance of utilizing an appropriate reference control tissue to understand canonical pathway alterations in AKI.
Engineering synthetic phosphorylation signaling networks in human cells
Authors: Yang Xiaoyu , Rocks Jason W. , Jiang Kaiyi , Walters Andrew J. , Rai Kshitij , Liu Jing , Nguyen Jason , Olson Scott D. , Mehta Pankaj , Collins James J. , Daringer Nichole M. , Bashor Caleb J. 
Published: September 2023

ABSTRACTProtein phosphorylation signaling networks play a central role in how cells sense and respond to their environment. Here, we describe the engineering of artificial phosphorylation networks in which “push-pull” motifs—reversible enzymatic phosphorylation cycles consisting of opposing kinase and phosphatase activities—are assembled from modular protein domain parts and then wired together to create synthetic phosphorylation circuits in human cells. We demonstrate that the composability of our design scheme enables model-guided tuning of circuit function and the ability to make diverse network connections; synthetic phosphorylation circuits can be coupled to upstream cell surface receptors to enable fast-timescale sensing of extracellular ligands, while downstream connections can regulate gene expression. We leverage these capabilities to engineer cell-based cytokine controllers that dynamically sense and suppress activated T cells. Our work introduces a generalizable approach for designing and building phosphorylation signaling circuits that enable user-defined sense-and- respond function for diverse biosensing and therapeutic applications.
Modeling flexible protein structure with AlphaFold2 and cross-linking mass spectrometry
Authors: Manalastas-Cantos Karen , Adoni Kish R. , Pfeifer Matthias , Märtens Birgit , Grünewald Kay , Thalassinos Konstantinos , Topf Maya 
Published: September 2023

AbstractWe propose a pipeline that combines AlphaFold2 (AF2) and crosslinking mass spectrometry (XL-MS) to model the structure of proteins with multiple conformations. The pipeline consists of two main steps: ensemble generation using AF2, and conformer selection using XL-MS data. For conformer selection, we developed two scores – the monolink probability score (MP) and the crosslink probability score (XLP), both of which are based on residue depth. We benchmarked MP and XLP on a large dataset of decoy protein structures, and showed that our scores outperform previously developed scores. We then tested our methodology on three proteins having an open and closed conformation in the Protein Data Bank: Complement component 3 (C3), luciferase, and glutamine-binding periplasmic protein (QBP), first generating ensembles using AF2, which were then screened for the open and closed conformations using experimental XL-MS data. In five out of six cases, the most accurate model within the AF2 ensembles – or a conformation within 1 Å of this model – was identified using crosslinks, as assessed through the XLP score. In the remaining case, only the monolinks (assessed through the MP score) successfully identified the open conformation of QBP. This serves as a compelling proof-of-concept for the effectiveness of monolinks. In contrast, the AF2 assessment score (pTM) was only able to identify the most accurate conformation in two out of six cases. Our results highlight the complementarity of AF2 with experimental methods like XL-MS, with the MP and XLP scores providing reliable metrics to assess the quality of the predicted models.
Genetic analysis of Indian sporadic young onset patient with Amyotrophic Lateral Sclerosis
Authors: Roychowdhury Saileyee , Joshi Deepika , Singh Vinay Kumar , Faruq Mohammed , Das Parimal 
Published: September 2023

AbstractBackgroundAmyotrophic lateral sclerosis (ALS) is an old onset devastating neurodegenerative disorder. Young-onset ALS cases especially sporadic ones who are between 25 and 45 years are rarely affected by the disease. Despite the identification of numerous candidate genes associated with ALS, the aetiology of the disease remains elusive due to extreme genetic and phenotypic variability. The advent of affordable whole exome sequencing has opened new avenues for unraveling the disease’s pathophysiology better.Methods and ResultsOur aim was to determine the genetic basis of an Indian-origin, young onset sporadic ALS patient with very rapid deterioration of the disease course without any cognitive decline who was screened for mutations in major ALS candidate genes by Whole exome sequencing. Variants detected were reconfirmed by Sanger Sequencing. The clinicopathological features were investigated and two heterozygous missense variants harboring each inANXA11,in one of the four conserved C terminal domains (R452W) and another in theSIGMAR1(R208W) were thus identified respectively. Both of these variants were predicted to be damaging by pathogenicity prediction tools and variousIn silicomethods.ConclusionsOur study revealed two potentially pathogenic variants in two ALS candidate genes. The genetic makeup of ALS patients from India has been the subject of a few prior studies, but none of them examinedANXA11andSIGMAR1genes so far. These results establish the framework for additional research into the pathogenic processes behind these variations that result in sporadic ALS disease and further our understanding of the genetic makeup of Indian ALS patients.
Cellular aging is accelerated in the malignant clone of myeloproliferative neoplasms
Authors: Vieri Margherita , Tharmapalan Vithurithra , Kalmer Milena , Baumeister Julian , Nikolić Miloš , Schnitker Matthis , Kirschner Martin , Flosdorf Niclas , de Toledo Marcelo A. S. , Zenke Martin , Koschmieder Steffen , Brümmendorf Tim H. , Beier Fabian , Wagner Wolfgang 
Published: September 2023

AbstractMyeloproliferative neoplasms (MPNs) are caused by somatic driver mutations, such asJAK2V617F, which might also affect cellular aging and senescence. Here, we analyzed the heterogeneity of aging in MPN patients and if this can be used to specifically target malignant cells. The mean epigenetic age was significantly accelerated in 129 MPN patients across all disease-entities, whereas premature telomere attrition was particularly observed in primary myelofibrosis. Overall, accelerated cellular aging correlated withJAK2V617Fallele frequency and was more pronounced in colony forming cells withJAK2V617Fas compared toJAK2wild- type colonies.JAK2V617Fmutation did not evoke clear acceleration of aging in syngeneic iPSC models upon short-term hematopoietic differentiation. On the other hand, a murineJak2V617Fmodel revealed epigenetic age-acceleration that therefore appears as sequel of disease progression. To investigate if the malignant clone might be targeted, we tested eight senolytic compounds, of which JQ1 and piperlongumine showed a reduction in allele burden and an increase in telomere length. Notably, treatment with the telomerase inhibitor BIBR-1532 reduced mutated colonies, particularly in patients with preexisting short telomeres. Our results indicate that cellular aging is accelerated in malignant MPN clones and this can provide a target for treatment with senolytic drugs or telomerase inhibitors.
Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions
Authors: Morimoto Kento , Suzuki Hayate , Kuno Akihiro , Daitoku Yoko , Tanimoto Yoko , Kato Kanako , Murata Kazuya , Sugiyama Fumihiro , Mizuno Seiya 
Published: September 2023

ABSTRACTFunctional regions that regulate biological phenomena are interspersed throughout eukaryotic genomes. The most definitive approach for identifying such regions is to confirm the phenotype of cells or organisms in which specific regions have been mutated or removed from the genome. This approach is invaluable for the functional analysis of genes with a defined functional element, the protein-coding sequence. In contrast, no functional analysis platforms have been established for the study of cis-elements or microRNA cluster regions consisting of multiple microRNAs with functional overlap. Whole-genome mutagenesis approaches, such as viaN-ethyl-N-nitrosourea (ENU) and gene trapping, have greatly contributed to elucidating the function of coding genes. These methods almost never induce deletions of genomic regions or multiple mutations within a narrow region. In other words, cis-elements and microRNA clusters cannot be effectively targeted in such a manner. Herein, we established a novel region-specific random mutagenesis method namedCRISPR-&Transposase-basedRegionaLMutagenesis (CTRL-Mutagenesis). We demonstrated that CTRL-mutagenesis randomly induces diverse mutations within target regions in murine embryonic stem cells. Comparative analysis of mutants harbouring subtly different mutations within the same region would facilitate the further study of cis-element and microRNA clusters.GRAPHICAL ABSTRACT
Citizen engagement and dialogic accounting through social media: a study of Italian regions
Authors: Contri Marco , Fissi Silvia , Gori Elena 
Published: September 2023

PurposeThis exploratory study aims to investigate the use of Facebook as a dialogic accounting tool for promoting citizen engagement in Italian regions.Design/methodology/approachThis study adopts a mixed methodology. Indeed, it first collects some quantitative data to construct an engagement index for the Facebook pages of the Italian regions, and then it performs a content analysis of some posts while also examining the tenor of the related comments and the level of interaction between regions and citizens.FindingsThe Italian regions have mainly used their Facebook pages for public communication purposes rather than for public participation. Therefore, they have conceived social pages more as an instrument of self-legitimisation and thus monologic accounting and have rarely considered them as a tool for engaging citizens who, in turn, showed low interest in participating in online debates. Nature and environment, tourism promotion and sport were the most engaging content types. Findings also confirm that posting many messages does not automatically increase engagement.Originality/valueThis study is one of the first to investigate the potential of social media from a dialogic accounting perspective, especially in the public sector. Additionally, it focuses on regions which are understudied in the literature, although they are critical actors in implementing public policies. Last but not least, this study offers a framework that integrates the literature on the use of social media for citizen engagement and research on such platforms as dialogic accounting tools.
Ability first or opportunity first in the m-health era? A hybrid SEM-ANN approach
Authors: Li Ping 
Published: September 2023

PurposeThe purpose of this study is to propose and test a model to explain users’ intention to adopt m-health devices and divide the importance of antecedents for users to adopt m-health devices based on the stimulus-organism-response (S-O-R) framework.Design/methodology/approachThis research conducted an online survey with m-health app users and collected 562 valid responses. A hybrid SEM-ANN approach was employed to evaluate the research model and hypotheses.FindingsThe results show that motivation (M), opportunity (O), and ability (A) affect users’ flow experience and loyalty and further affect their adoption intention of m-health technology. Opportunity plays a more critical role in m-health adoption intention than ability.Originality/valueThis study comprehensively examined the factors that affect users’ deep engagement and m-health adoption from the perspective of MOA. It used the hybrid SEM-ANN method to divide the critical role of motivation, opportunity and ability, providing a new analysis approach for studying information technology (IT) behavior.
Exploring the influence of entrepreneurial identity on students’ choice between entrepreneurship courses and university-based incubators
Authors: Masoumi Mehdi (Mohammadmehdi) 
Published: September 2023

Purpose
The purpose of this study is to answer the following questions. What kind of entrepreneurial identities do students have that motivate them to choose either of the entrepreneurship course and university-based incubator? How do students involve in the entrepreneurship ecosystem at university based on their entrepreneurial identity?

Design/methodology/approach
For this study, the author began to gather information using previous knowledge and any aspect of a work, namely, from the literature review to represent interpretive syntheses of the meaning-making literature review addressing the research question.

Findings
This study suggests what happens to entrepreneur students from academia and the reason that they end up in one of the two aforementioned paradigms. This paper aims to underpin the issue of how various entrepreneurial identities of students cause substantial contributing factors in forming such entrepreneurial activities at university and throughout the entire innovation ecosystem.

Research limitations/implications
Almost all of the content of the entrepreneurship education (EE) courses and incubator training is oriented towards consensual entrepreneurship methods, in accordance with entrepreneurship education. Although the core contents of the EE courses and university-based incubators’ training are the same, the outcomes are quite different.

Originality/value
This study considers the students’ entrepreneurial identities with a focus on their point of view that led them to end up in one of the two common entrepreneurship resources at universities: the EE course and entrepreneurial activities related to university-based incubators.