bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of evernic acid on human breast cancer MCF‐7 and MDA‐MB‐453 cell lines via thioredoxin reductase 1: A molecular approach
Authors: Kalın Şeyda Nur , Altay Ahmet , Budak Harun 
Published: February 2023

AbstractThioredoxin reductase 1 (TrxR1) has emerged as an important target for anticancer drug development due to its overexpression in many human tumors including breast cancer. Due to the serious side effects of currently used commercial anticancer drugs, new natural compounds with very few side effects and high efficacy are of great importance in cancer treatment. Lichen secondary metabolites, known as natural compounds, have diverse biological properties, including antioxidant and anticancer activities. Herein, we aimed to determine the potential antiproliferative, antimigratory, and apoptotic effects of evernic acid, a lichen secondary metabolite, on breast cancer MCF‐7 and MDA‐MB‐453 cell lines and afterward to investigate whether its anticancer effect is exerted by TrxR1‐targeting. The cytotoxicity results indicated that evernic acid suppressed the proliferation of MCF‐7 and MDA‐MB‐453 cells in a dose‐dependent manner and the IC50 values were calculated as 33.79 and 121.40 μg/mL, respectively. Migration assay results revealed the notable antimigratory ability of evernic acid against both cell types. The expression of apoptotic markers Bcl2 associated X, apoptosis regulator, Bcl2 apoptosis regulator, and tumor protein p53 by quantitative real‐time polymerase chain reaction and western blot analysis showed that evernic acid did not induce apoptosis in both cell lines, consistent with flow cytometry results. Evernic acid showed its anticancer effect via inhibiting TrxR1 enzyme activity rather than mRNA and protein expression levels in both cell lines. In conclusion, these findings suggest that evernic acid has the potential to be evaluated as a therapeutic agent in breast cancer treatment.
Therapeutic effects of atorvastatin on doxorubicin‐induced hepatotoxicity in rats via antioxidative damage, anti‐inflammatory, and anti‐lipotoxicity
Authors: Wu Yan‐Zhao , Wang Ke‐Xin , Ma Xin‐di , Wang Chu‐Chu , Chen Nan‐Nan , Xiong Chen , Li Jun‐Xia , Su Su‐Wen 
Published: February 2023

AbstractDoxorubicin (DOX), is a high efficiency anthracycline antitumor drug. However, the clinical application of DOX is limited mainly by dose‐related adverse drug reactions. Currently, the therapeutic effects of Atorvastatin (ATO) on DOX‐induced hepatotoxicity were studied in vivo. The results indicated that DOX impaired hepatic function, as measured by an increased levels of liver weight index and serum concentrations of aspartate transaminase and alanine transaminase, as well as alteration of hepatic histology. In addition, DOX increased the serum levles of triglyceride (TG) and nonestesterified fatty acid. ATO prevented these changes. Mechanical analysis revealed that ATO restored the changes of malondialdehyde, reactive oxygen radical species, glutathione peroxidase and manganese superoxide dismutase. Additionally, ATO inhibited the increased expression levels of nuclear factor‐kappa B and interleukin 1β, hence suppressing inflammation. Meanwhile, ATO inhibited cell apoptosis by dramatically decreasing the Bax/Bcl‐2 ratio. In addition, ATO mitigated the lipidtoxicity by inhibiting the adipolysis of TG and accelerating hepatic lipid metabolism. Taken together, the results suggest ATO has therapeutic effect on DOX‐induced hepatotoxicity via inhibition of oxidative damage, inflammatory and apoptosis. In addition, ATO attenuates DOX‐induced hyperlipidemia via modulation of lipid metabolism.
Kylin 1.0: An ab‐initio density matrix renormalization group quantum chemistry program
Authors: Xie Zhaoxuan , Song Yinxuan , Peng Fangwen , Li Jianhao , Cheng Yifan , Zhang Lingzhi , Ma Yingjin , Tian Yingqi , Luo Zhen , Ma Haibo 
Published: February 2023

AbstractThe accurate evaluation of electron correlations is highly necessary for the proper descriptions of the electronic structures in strongly correlated molecules, ranging from bond‐dissociating molecules, polyradicals, to large conjugated molecules and transition metal complexes. For this purpose, in this paper, a new ab‐initio quantum chemistry program Kylin 1.0 for electron correlation calculations at various quantum many‐body levels, including configuration interaction (CI), perturbation theory (PT), and density matrix renormalization group (DMRG), is presented. Furthermore, fundamental quantum chemistry methods such as Hartree‐Fock self‐consistent field (HF‐SCF) and the complete active space SCF (CASSCF) are also implemented. The Kylin 1.0 program possesses the following features: (1) a matrix product operator (MPO) formulation‐based efficient DMRG implementation for describing static electron correlation within a large active space composed of more than 100 orbitals, supporting both and symmetries; (2) an efficient second‐order DMRG‐self‐consistent field (SCF) implementation; (3) an externally contracted multi‐reference CI (MRCI) and Epstein‐Nesbet PT with DMRG reference wave functions for including the remaining dynamic electron correlation outside the large active spaces. In this paper, we introduce the capabilities and numerical benchmark examples of the Kylin 1.0 program.
Evaluation of the Impact of Ritlecitinib on Organic Cation Transporters Using Sumatriptan and Biomarkers as Probes
Authors: Wang Xiaoxing , Purohit Vivek , Dowty Martin E. , Rodrigues David , Luo Lina , Mathialagan Sumathy , Carey William , Plotka Anna , Kalluru Hindu , Melissa O'Gorman , Kaplan Julia , Huh Yeamin , Vourvahis Manoli , Wolk Robert M. 
Published: February 2023

ABSTRACTRitlecitinib, an inhibitor of Janus kinase 3 and hepatocellular carcinoma family kinases, is in development as potential treatment for several inflammatory diseases. In vitro studies presented ritlecitinib as an inhibitor of hepatic organic cation transporter (OCT) 1, renal transporters OCT2 and multidrug and toxin extrusion (MATE) proteins 1/2K using multiple substrates, and ritlecitinib's major inactive metabolite M2, as an inhibitor of OCT1. A clinical interaction study with an OCT1 drug probe (sumatriptan) and relevant probe biomarkers for OCT/MATE was conducted to assess the effect of ritlecitinib on these transporters in healthy adult participants. The selectivity of sumatriptan for OCT1 was confirmed through a series of in vitro uptake assays. A simple static model was used to help contextualize the observed changes in sumatriptan area under the plasma concentration‐time curve (AUC). Coadministration of a single 400‐mg dose of ritlecitinib increased sumatriptan AUC from time 0 to infinity (AUCinf) by ≈30% relative to a single 25‐mg sumatriptan administration alone. When administered 8 hours after a ritlecitinib dose, sumatriptan AUCinf increased by ≈50% relative to sumatriptan given alone. Consistent with OCT1 inhibition, the AUC from time 0 to 24 hours of isobutyryl‐L‐carnitine decreased by ≈15% after ritlecitinib. Based on the evaluation of the renal clearance of N1‐methylnicotinamide, ritlecitinib does not exert clinically meaningful inhibition on renal OCT2 or MATE1/2K. This study confirmed that ritlecitinib and M2 are inhibitors of OCT1 but not OCT2 or MATE1/2K in healthy adults.
Association of anorexia/appetite loss with malnutrition and mortality in older populations: A systematic literature review
Authors: Fielding Roger A. , Landi Francesco , Smoyer Karen E. , Tarasenko Lisa , Groarke John 
Published: February 2023

AbstractAnorexia/appetite loss in older subjects is frequently underrecognized in clinical practice, which may reflect deficient understanding of clinical sequelae. Therefore, we performed a systematic literature review to assess the morbidity and mortality burden of anorexia/appetite loss in older populations. Following PRISMA guidelines, searches were run (1 January 2011 to 31 July 2021) in PubMed, Embase® and Cochrane databases to identify English language studies of adults aged ≥ 65 years with anorexia/appetite loss. Two independent reviewers screened titles, abstracts and full text of identified records against pre‐defined inclusion/exclusion criteria. Population demographics were extracted alongside risk of malnutrition, mortality and other outcomes of interest. Of 146 studies that underwent full‐text review, 58 met eligibility criteria. Most studies were from Europe (n = 34; 58.6%) or Asia (n = 16; 27.6%), with few (n = 3; 5.2%) from the United States. Most were conducted in a community setting (n = 35; 60.3%), 12 (20.7%) were inpatient based (hospital/rehabilitation ward), 5 (8.6%) were in institutional care (nursing/care homes) and 7 (12.1%) were in other (mixed or outpatient) settings. One study reported results separately for community and institutional settings and is counted in both settings. Simplified Nutritional Appetite Questionnaire (SNAQ Simplified, n = 14) and subject‐reported appetite questions (n = 11) were the most common methods used to assess anorexia/appetite loss, but substantial variability in assessment tools was observed across studies. The most commonly reported outcomes were malnutrition and mortality. Malnutrition was assessed in 15 studies, with all reporting a significantly higher risk of malnutrition in older individuals with anorexia/appetite loss (vs. without) regardless of country or healthcare setting (community n = 9, inpatient n = 2, institutional n = 3, other n = 2). Of 18 longitudinal studies that assessed mortality risk, 17 (94%) reported a significant association between anorexia/appetite loss and mortality regardless of either healthcare setting (community n = 9, inpatient n = 6, institutional n = 2) or method used to assess anorexia/appetite loss. This association between anorexia/appetite loss and mortality was observed in cohorts with cancer (as expected) but was also observed in older populations with a range of comorbid conditions other than cancer. Overall, our findings demonstrate that, among individuals aged ≥ 65 years, anorexia/appetite loss is associated with increased risk of malnutrition, mortality and other negative outcomes across community, care home and hospital settings. Such associations warrant efforts to improve and standardize screening, detection, assessment and management of anorexia/appetite loss in older adults.
Biotreatment of discarded fruit juices and simultaneous lipid production using Leptolyngbya sp.
Authors: Patrinou Vasiliki , Antonopoulou Georgia , Ntaikou Ioanna , Tekerlekopoulou Athanasia G. 
Published: February 2023

AbstractBACKGROUNDFruit juices are an exceptional source of nutrients; however, due to their short shelf‐life and vulnerability to microbial degradation, large volumes of juice are regularly discarded worldwide. As a wastewater, discarded fruit juices (DFJ) possess high organic loads with significant amounts of nitrogen and phosphorus, making them a suitable substrate for microbial growth and to produce natural bioactive compounds.RESULTSDiscarded fruit juices (DFJ) were treated and simultaneously valorized (producing microbial lipids) using a microbial consortium dominated by the photosynthetic cyanobacterium Leptolyngbya sp. The effects of N:P and C:N ratios on growth and lipid production were examined, along with the ability of the consortium to biotreat DFJ in laboratory‐scale (LS) reactors. Using the optimum nutrient ratios (N:P 5:1, C:N 50.2:1), scaled‐up reactors (aquariums) were then tested under suspended and attached growth conditions. The biomass produced was further exploited for lipid production and the results revealed that Leptolyngbya sp. was able to produce up to 19% d.w. lipids and simultaneously efficiently treat DFJ. High dissolved‐chemical oxygen demand (d‐COD), total nitrogen, and PO43− removal rates (85.0% and 93.9%, 93.4% and 95.2%, 84.6% and 83.9%, respectively) were achieved in both the suspended and attached growth systems, along with high biomass productivities (316.3, 340.0 mg L−1 day−1, respectively).CONCLUSIONThe biological approach used in this study demonstrated that is possible to exploit DFJ and achieve satisfactory lipid contents (up to 19% d.w.) using a cyanobacterial‐based consortium. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Categorical versus dimensional structure of autism spectrum disorder: A multi‐method investigation
Authors: Frazier Thomas W. , Chetcuti Lacey , Al‐Shaban Fouad A. , Haslam Nick , Ghazal Iman , Klingemier Eric W. , Aldosari Mohammed , Whitehouse Andrew J. O. , Youngstrom Eric A. , Hardan Antonio Y. , Uljarević Mirko 
Published: February 2023

AbstractBackgroundA key question for any psychopathological diagnosis is whether the condition is continuous or discontinuous with typical variation. The primary objective of this study was to use a multi‐method approach to examine the broad latent categorical versus dimensional structure of autism spectrum disorder (ASD).MethodData were aggregated across seven independent samples of participants with ASD, other neurodevelopmental disorders (NDD), and non‐ASD/NDD controls (aggregate Ns = 512–16,755; ages 1.5–22). Scores from four distinct phenotype measures formed composite “indicators” of the latent ASD construct. The primary indicator set included eye gaze metrics from seven distinct social stimulus paradigms. Logistic regressions were used to combine gaze metrics within/across paradigms, and derived predicted probabilities served as indicator values. Secondary indicator sets were constructed from clinical observation and parent‐report measures of ASD symptoms. Indicator sets were submitted to taxometric‐ and latent class analyses.ResultsAcross all indicator sets and analytic methods, there was strong support for categorical structure corresponding closely to ASD diagnosis. Consistent with notions of substantial phenotypic heterogeneity, the ASD category had a wide range of symptom severity. Despite the examination of a large sample with a wide range of IQs in both genders, males and children with lower IQ were over‐represented in the ASD category, similar to observations in diagnosed cases.ConclusionsOur findings provide strong support for categorical structure corresponding closely to ASD diagnosis. The present results bolster the use of well‐diagnosed and representative ASD groups within etiologic and clinical research, motivating the ongoing search for major drivers of the ASD phenotype. Despite the categorical structure of ASD, quantitative symptom measurements appear more useful for examining relationships with other factors.
Hyperbolic modeling and assessment of hypothetical health behaviors during a viral outbreak using crowdsourced samples
Authors: Rzeszutek Mark J. , DeFulio Anthony , Brown Hayley D. , Cardoso São Mateus Cristal 
Published: February 2023

AbstractThe COVID‐19 pandemic provided an opportunity to investigate factors related to public response to public health measures, which could help better prepare implementation of similar measures for inevitable future pandemics. To understand individual and environmental factors that influence likelihood in engaging in personal and public health measures, three crowdsourced convenience samples from Amazon Mechanical Turk (MTurk) completed likelihood‐discounting tasks of engaging in health behaviors given a variety of hypothetical viral outbreak scenarios. Experiment 1 assessed likelihood of mask wearing for a novel virus. Experiment 2 assessed vaccination likelihood based on efficacy and cost. Experiment 3 assessed likelihood of seeking health care based on number of symptoms and cost of treatment. Volume‐based measures and three‐dimensional modeling were used to analyze hypothetical decision making. Hypothetical public and personal health participation increased as viral fatality increased and generally followed a hyperbolic function. Public health participation was moderated by political orientation and trust in science, whereas treatment‐seeking was only moderated by income. Analytic methods used in this cross‐sectional study predicted population‐level outcomes that occurred later in the pandemic and can be extended to various health behaviors.
Verbal behavior development theory and relational frame theory: Reflecting on similarities and differences
Authors: Sivaraman Maithri , Barnes‐Holmes Dermot , Greer R. Douglas , Fienup Daniel M. , Roeyers Herbert 
Published: February 2023

AbstractRelational frame theory and verbal behavior development theory are two behavior‐analytic perspectives on human language and cognition. Despite sharing reliance on Skinner's analysis of verbal behavior, relational frame theory and verbal behavior development theory have largely been developed independently, with initial applications in clinical psychology and education/development, respectively. The overarching goal of the current paper is to provide an overview of both theories and explore points of contact that have been highlighted by conceptual developments in both fields. Verbal behavior development theory research has identified how behavioral developmental cusps make it possible for children to learn language incidentally. Recent developments in relational frame theory have outlined the dynamic variables involved across the levels and dimensions of arbitrarily applicable relational responding, and we argue for the concept of mutually entailed orienting as an act of human cooperation that drives arbitrarily applicable relational responding. Together these theories address early language development and children's incidental learning of names. We present broad similarities between the two approaches in the types of functional analyses they generate and discuss areas for future research.
Morphology and glandular composition of the myzorhynchus and the remnant apical organ in adult cestodes of the order Rhinebothriidea from batoids off Argentina
Authors: Franzese Sebastián , Mutti Leonardo D. , Battista Ariadna G. , Ivanov Verónica A. 
Published: February 2023

AbstractThe main objective of this study was to analyze the function of the myzorhynchus and remnant apical organ of adult cestodes in the order Rhinebothriidea. Several features of these structures were analyzed in 12 species belonging to six genera and two families. In particular, the glandular composition of the myzorhynchus of four species from Echeneibothriidae (i.e., Notomegarhynchus navonae and three species of Echeneibothrium) was studied using histochemical techniques and/or transmission electron microscopy. In addition, the presence of a remnant apical organ and its glandular composition were analized in six species of Rhinebothriidae and in two species of Semiorbiseptum, whose familial assignment is uncertain. We also evaluated the importance of these characters for diagnosis. The same type of gland cell was found in the myzorhynchus of Echeneibothrium species and in the remnant apical organ of Semiorbiseptum species. These gland cells were Coomassie brilliant blue‐positive, periodic acid Schiff‐positive and Alcian blue‐negative, consistent with a glycoprotein secretion possibly involved in adhesion to the host mucosa and proteolysis. The type of gland cells found in the myzorhynchus of N. navonae were Coomassie brilliant blue‐negative, periodic acid Schiff‐positive and Alcian blue‐positive, consistent with the production of adhesive and protective substances. The type of gland cells in the myzorhynchus and in the remnant apical organ could be a useful character for the generic diagnosis of Echeneibothrium and Semiorbiseptum, respectively. A remnant apical organ was only found in Semiorbiseptum, with its presence/absence being important as a diagnostic character at the generic level for Semiorbiseptum, Scalithrium, and Rhinebothroides. A secondary objective was to characterize the microthrix pattern of the myzorhynchus of N. navonae. An extended distribution of spinitriches was detected, which may allow a better adhesion of this large species to the host mucosa, as the main function of spinitriches is presumably that of adhesion.
Gilteritinib: Repurposing of AXL‐targeting kinase inhibitors against COVID‐19
Authors: Chen Yisa , Xue Yiying , Yang Jing 
Published: February 2023

AbstractThe coronavirus disease‐19 (COVID‐19) is an ongoing infection outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), a novel positive single‐stranded, enveloped RNA virus belonging to the genus Betacoronavirus. During the pandemic, the SARS‐CoV‐2 subvariants evolved rapidly with enhanced transmissibility, and became a global public health threat as its alarmingly rising rate of infection led to excessive mortality. According to the WHO data, COVID‐19 caused more than 6 million deaths and affected 215 countries. Although vaccines are beneficial for preventing hospitalization, reducing severe illness and deaths from COVID‐19, the constantly mutated Spike protein under high selection pressure leading to off‐target or immune evasion which warrants additional therapeutic strategies. Therefore, it is important to identify and test potential therapeutic targets against proteins that are highly conserved among multiple coronaviruses for clinical drug development to combat SARS‐COV‐2. While research for new therapies continues, the cost‐effective and rapid repurposing of existing therapeutics may provide a viable treatment alternative for COVID‐19.
Survival rates of band‐tailed pigeons estimated using passive integrated transponder tags
Authors: Collins Daniel P. , Zimmerman Guthrie S. , Carleton Scott A. , Kendall William L. , Coxen Christopher L. 
Published: February 2023

AbstractObtaining survival estimates on the Interior population of band‐tailed pigeons (Patagioenas fasciata) is challenging because they are trap shy, but the joint use of passive integrated transponder (PIT) tags and bands is a potential solution. We investigated the use of PIT tags to passively recapture band‐tailed pigeon at 3 locations in New Mexico, USA, to estimate survival. From 2013–2015, we captured, banded, and marked >600 individual band‐tailed pigeons with PIT tags. To estimate annual survival rates, we used a Barker multi‐state joint live and dead encounters and resighting model. Survival models excluding transience had survival estimates across site, sex, and year of 0.86 (95% CI = 0.84–0.88) for after hatch year birds and 0.63 (95% CI = 0.48–0.76) for hatch year birds. These results are consistent with other survival estimates reported for the Interior population of band‐tailed pigeons using band return data and potentially provide an effective alternative method of monitoring survival of this population.
Mussel Inspired In Situ Preparation of Antibacterial Silver Nanoparticles by DOPA‐Containing Silk Fibroin
Authors: Wu Yuting , Chen Chan , Tang Weikang , Liu Wenbin 
Published: February 2023

AbstractSilver nanoparticles (AgNPs) attract a great deal of attention for potent antibacterial capacity, but their use is challenged by limited stability. Inspired by the adhesive and redox properties of the mussel foot proteins containing L‐3,4‐dihydroxyphenylalanine (DOPA), a facile strategy for in situ synthesis of AgNPs using DOPA‐containing fibroin is developed. Tyrosine residues in fibroin are transformed into DOPA via biomimetic synthesis method with content of 0.55 mol%. In situ synthesis generates stable and small AgNPs through DOPA bound in fibroin as a reducing and stabilizing agent. Narrow size distribution with average diameter of 20 nm and excellent monodispersity are obtained. Cross‐linking with lysine increases the content of β‐sheet to form hydrogel and achieves gradual release of silver. The material exhibits excellent antibacterial properties against Gram‐positive Staphylococcus aureus and Gram‐negative Escherichia coli bacteria. It can be potentially applied in biological and medical fields to treat bacterial infections.