bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface electroencephalographic neurofeedback improves sustained attention in ADHD: a meta-analysis of randomized controlled trials
Authors: Chiu Hsien‐Jane , Sun Cheuk-Kwan , Fan Hsin-Yi , Tzang Ruu‐Fen , Wang Ming-Yu , Cheng Ying-Chih , Cheng Yu-Shian , Yeh Pin-Yang , Chung Weilun 
Published: December 2022

Abstract
Background
The efficacy of surface electroencephalographic neurofeedback (EEG-NF) for improving attentional performance assessed by laboratory measures in patients with attention-deficit/hyperactivity disorder (ADHD) remains unclear.

Methods
Following the PRISMA guidelines, the PubMed, Embase, ClinicalKey, Cochrane CENTRAL, ScienceDirect, Web of Science, and ClinicalTrials.gov databases were systematically searched for randomized controlled trials on the efficacy of surface EEG-NF against ADHD focusing on attentional performance evaluated by laboratory measures from inception to January 2022.

Results
Fourteen eligible studies were analyzed. Of the 718 participants involved, 429 diagnosed with ADHD received EEG-NF treatment. Significant improvement in attentional performance in ADHD subjects receiving EEG-NF was noted compared to their comparators (p < 0.01). Besides, there was a significant EEG-NF-associated beneficial effect on sustained attention (Hedges’ g = 0.32, p < 0.01), whereas the impact on selective attention (p = 0.57) and working memory (p = 0.59) was limited. Moreover, protocol including beta wave enhancement was superior to that only focusing on reducing theta/beta ratio or modulation of slow cortical potential. Subgroup analyses showed that three sessions per week of EEG-NF produced the best effect, while the efficacy of surface EEG-NF was much poorer (Hedges’ g = 0.05) when only studies that blinded their participants from knowledge of treatment allocation were included. No significant difference was noted in the improvement of attentional performance 6–12 months after EEG-NF intervention (n = 3, p = 0.42).

Conclusions
Our results demonstrated the satisfactory effectiveness of surface EEG-NF for improving sustained attention, especially when beta wave enhancement was included, despite its failure to sustain a long-term effect. Further large-scale trials are warranted to support our findings.
Single-cell transcriptome reveals dominant subgenome expression and transcriptional response to heat stress in Chinese cabbage
Authors: Sun Xiaoxue , Feng Daling , Liu Mengyang , Qin Ruixin , Li Yan , Lu Yin , Zhang Xiaomeng , Wang Yanhua , Shen Shuxing , Ma Wei , Zhao Jianjun 
Published: December 2022

Abstract
Background
Chinese cabbage (Brassica rapa ssp. pekinensis) experienced a whole-genome triplication event and thus has three subgenomes: least fractioned, medium fractioned, and most fractioned subgenome. Environmental changes affect leaf development, which in turn influence the yield. To improve the yield and resistance to different climate scenarios, a comprehensive understanding of leaf development is required including insights into the full diversity of cell types and transcriptional networks underlying their specificity.

Results
Here, we generate the transcriptional landscape of Chinese cabbage leaf at single-cell resolution by performing single-cell RNA sequencing of 30,000 individual cells. We characterize seven major cell types with 19 transcriptionally distinct cell clusters based on the expression of the reported marker genes. We find that genes in the least fractioned subgenome are predominantly expressed compared with those in the medium and most fractioned subgenomes in different cell types. Moreover, we generate a single-cell transcriptional map of leaves in response to high temperature. We find that heat stress not only affects gene expression in a cell type-specific manner but also impacts subgenome dominance.

Conclusions
Our study highlights the transcriptional networks in different cell types and provides a better understanding of transcriptional regulation during leaf development and transcriptional response to heat stress in Chinese cabbage.
Persisting neuropsychiatric symptoms, Alzheimer’s disease, and cerebrospinal fluid cortisol and dehydroepiandrosterone sulfate
Authors: Ouanes Sami , Rabl Miriam , Clark Christopher , Kirschbaum Clemens , Popp Julius 
Published: December 2022

Abstract
Introduction
Neuropsychiatric symptoms are important treatment targets in the management of dementia and can be present at very early clinical stages of neurodegenerative diseases. Increased cortisol has been reported in Alzheimer’s disease (AD) and has been associated with faster cognitive decline. Elevated cortisol output has been observed in relation to perceived stress, depression, and anxiety. Dehydroepiandrosterone sulfate (DHEAS) has known anti-glucocorticoid effects and may counter the effects of cortisol.

Objectives
We aimed to examine whether CSF cortisol and DHEAS levels were associated with (1) neuropsychiatric symptoms at baseline, (2) changes in neuropsychiatric symptoms over 3 years, and (3) whether these associations were related to or independent of AD pathology.

Methods
One hundred and eighteen participants on a prospective study in a memory clinic setting, including patients with cognitive impairment (n = 78), i.e., mild cognitive impairment or mild dementia, and volunteers with normal cognition (n = 40), were included. Neuropsychiatric symptoms were assessed using the Neuropsychiatric Inventory Questionnaire (NPI-Q). CSF cortisol and DHEAS, as well as CSF AD biomarkers, were obtained at baseline. Neuropsychiatric symptoms were re-assessed at follow-up visits 18 and 36 months from baseline. We constructed linear regression models to examine the links between baseline neuropsychiatric symptoms, the presence of AD pathology as indicated by CSF biomarkers, and CSF cortisol and DHEAS. We used repeated-measures mixed ANCOVA models to examine the associations between the neuropsychiatric symptoms’ changes over time, baseline CSF cortisol and DHEAS, and AD pathology.

Results
Higher CSF cortisol was associated with higher NPI-Q severity scores at baseline after controlling for covariates including AD pathology status (B = 0.085 [0.027; 0.144], p = 0.027; r = 0.277). In particular, higher CSF cortisol was associated with higher baseline scores of depression/dysphoria, anxiety, and apathy/indifference. Elevated CSF cortisol was also associated with more marked increase in NPI-Q scores over time regardless of AD status (p = 0.036, η2 = 0.207), but this association was no longer significant after controlling for BMI and the use of psychotropic medications. CSF DHEAS was associated neither with NPI-Q scores at baseline nor with their change over time. Cortisol did not mediate the association between baseline NPI-Q and changes in clinical dementia rating sum of boxes over 36 months.

Conclusion
Higher CSF cortisol may reflect or contribute to more severe neuropsychiatric symptoms at baseline, as well as more pronounced worsening over 3 years, independently of the presence of AD pathology. Our findings also suggest that interventions targeting the HPA axis may be helpful to treat neuropsychiatric symptoms in patients with dementia.
PD-1 and LAG-3-positive T cells are associated with clinical outcomes of relapsed/refractory multiple myeloma patients
Authors: Chen Ming , Zhu Jinlian , Yang Xuedong , Yao Jianxin , Liu Yuqing , Liu Qiang 
Published: December 2022

Abstract
Objective
To investigate the frequency of PD-1 and LAG-3-positive T cells in relapsed/refractory multiple myeloma (RRMM) patients and its clinical significance.

Methods
This prospective observational study enrolled a total of 71 RRMM patients, as well as 70 MM patients (non-refractory) and 70 healthy individuals during January 2018 to March 2021. The frequency of circulating CD4+ and CD8+ T cells expressing PD-1 and LAG-3 was analyzed using flow cytometry. Serum cytokines of IL-6, IL-17, CRP, TNF-α and TGF-β were evaluated by enzyme linked immunosorbent assay (ELISA).

Results
Significant higher 1-year mortality rate was found in RRMM patients compared with the MM patients. In both CD4+ and CD8+ T cells, the frequencies of PD-1+, LAG-3+ and PD-1+/LAG-3+ T cells were markedly higher in the RRMM patients and the deceased patients, compared with the MM patients and the survival patients, respectively. All cytokines were remarkably higher in RRMM and MM patients than in the healthy control, while only serum levels of IL-6 and IL-17 were markedly higher in RRMM patients compared with the MM patients. Positive correlation was observed among the IL-6, IL-17 and the frequencies of circulating T cells in both CD4+ and CD8+ T cells in RRMM and MM patients. The frequency of CD8+PD-1+LAG-3+ T cells showed the best sensitivity 82.61% and specificity 76.06% for diagnosis of RRMM using ROC curve. Meanwhile, the frequency of CD4+PD-1+ cells showed the best sensitivity 84.00% and specificity 97.35% for prediction of patients’ mortality by ROC curve. The frequencies of CD4+PD-1+, CD8+PD-1+/LAG-3+, as well as IL-6, IL-17 and TNF-α were found as risk factors for incidence of RRMM in all MM patients.

Conclusion
The frequency of PD-1 and LAG-3-positive T cells is associated with the clinical severity and inflammation in RRMM patients, which may also serve as potential biomarkers for its diagnosis.
Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites
Authors: Wang Shujin , Fu Wei , Zhao Xueya , Chang Xiaojiao , Liu Hujun , Zhou Lin , Li Jian , Cheng Rui , Wu Xin , Li Xi , Sun Changpo 
Published: December 2022

Abstract
Background
Exposure to zearalenone (ZEN, a widespread Fusarium mycotoxin) causes reproductive toxicity and immunotoxicity in farm animals, and it then poses potential threats to human health through the food chain. A systematic understanding of underlying mechanisms on mycotoxin-induced toxicity is necessary for overcoming potential threats to farm animals and humans. The gastrointestinal tract is a first-line defense against harmful mycotoxins; however, it remains unknown whether mycotoxin (e.g., ZEN)-induced toxicity on the reproductive-immune axis is linked to altered gut microbial metabolites. In this study, using pigs (during the three phases) as an important large animal model, we investigated whether ZEN-induced toxicity on immune defense in the reproductive-immune axis was involved in altered gut microbial-derived metabolites. Moreover, we observed whether the regulation of gut microbial-derived metabolites through engineering ZEN-degrading enzymes counteracted ZEN-induced toxicity on the gut-reproductive-immune axis.

Results
Here, we showed ZEN exposure impaired immune defense in the reproductive-immune axis of pigs during phase 1/2. This impairment was accompanied by altered gut microbial-derived metabolites [e.g., decreased butyrate production, and increased lipopolysaccharides (LPS) production]. Reduction of butyrate production impaired the intestinal barrier via a GPR109A-dependent manner, and together with increased LPS in plasma then aggravated the systemic inflammation, thus directly and/or indirectly disturbing immune defense in the reproductive-immune axis. To validate these findings, we further generated recombinant Bacillus subtilis 168-expressing ZEN-degrading enzyme ZLHY-6 (the Bs-Z6 strain) as a tool to test the feasibility of enzymatic removal of ZEN from mycotoxin-contaminated food. Notably, modified gut microbial metabolites (e.g., butyrate, LPS) through the recombinant Bs-Z6 strain counteracted ZEN-induced toxicity on the intestinal barrier, thus enhancing immune defense in the reproductive-immune axis of pigs during phase-3. Also, butyrate supplementation restored ZEN-induced abnormalities in the porcine small intestinal epithelial cell.

Conclusions
Altogether, these results highlight the role of gut microbial-derived metabolites in ZEN-induced toxicity on the gut-reproductive-immune axis. Importantly, targeting these gut microbial-derived metabolites opens a new window for novel preventative strategies or therapeutic interventions for mycotoxicosis associated to ZEN.
Temporal and spatial patterns and influencing factors of intangible cultural heritage: Ancient Qin-Shu roads, Western China
Authors: Liu Yuan , Chen Mo , Tian Yonggang 
Published: December 2022

Abstract
The ancient Qin-Shu roads corridor is one of the important cultural main corridors in China. Throughout China’s long historical and cultural evolution, today’s ancestors created a rich intangible cultural heritage along this route. Studying its intangible cultural heritage has important theoretical and practical significance for the protection and innovation of cultural heritage in this region. The purpose of this study is to analyze the spatial and temporal distribution characteristics of intangible cultural heritage along the ancient Qin-Shu roads and explore the main factors affecting its distribution. The nearest neighbor index, kernel density estimation, standard deviation ellipse, location entropy, buffer analysis and other methods were used. The results show that (1) The types of intangible cultural heritage of the ancient Qin-Shu roads are expressed in three echelons. Traditional handicrafts are the most numerous, folk custom and traditional music are the second most numerous, the other categories of ICH are third in quantity overall, among which traditional medicine and sports recreation competition are the scarcest. (2) The overall spatial distribution of intangible cultural heritage along the ancient Qin-Shu roads shows an agglomeration distribution. Its distribution pattern places the central cities (Xi'an, Chengdu and Chongqing) at the core, gradually spreading out and decreasing in density as it reaches peripheral districts and counties. There are significantly differ in the core areas of different types of intangible cultural heritage. (3) In the process of historical development, the intangible cultural heritage of the ancient Qin-Shu roads demonstrated an overall change pattern of “three rising and three falling”. That is, during Qin and Han Dynasties, Sui, Tang and Five Dynasties, and Ming and Qing Dynasties, culture flourished, while in Wei, Jin, Southern and Northern Dynasties, Song and Yuan Dynasties, modern times culture developed slowly. The overall trajectory of the center of gravity of intangible cultural heritage shifted from the northeast to the southwest. (4) Natural and human factors, such as topography, climate, transportation, traditional villages and population evolution, have an important impact on the spatial pattern of the intangible cultural heritage of the ancient Qin-Shu roads. The results of this study provide a useful reference for the theoretical research and practical management of intangible cultural heritage.
Three-dimensional evaluation of maxillary tooth movement in extraction patients with three different miniscrew anchorage systems: a randomized controlled trial
Authors: Zhang Liwen , Guo Runzhi , Xu Baohua , Wang Yue , Li Weiran 
Published: December 2022

Abstract
Objective
To compare the three-dimensional (3-D) movement of maxillary teeth in response to three common miniscrew anchorage systems in extraction patients with maxillary dentoalveolar protrusion.

Materials and methods
The study employed a randomized controlled single-blinded design with three arms. Thirty extraction patients who required maximum anchorage to retract maxillary anterior teeth were included and randomly allocated into three treatment groups: space closure with direct miniscrew anchorage and low crimpable hooks (DL group), indirect miniscrew anchorage and low crimpable hooks (IL group), and direct miniscrew anchorage and high crimpable hooks (DH group). Cone beam computed tomography (CBCT) images of all included patients were obtained immediately before (T0) and after (T1) space closure. The outcomes were 3-D positional changes of maxillary central incisor, lateral incisor, canine, second premolar, and first molar. The repeated measures analysis of variance with post hoc LSD test was used to evaluate differences among groups.

Results
A significant intrusion (− 1.34 mm; 95% CI, − 1.60 mm, 1.08 mm) and buccal (− 6.92°; 95% CI, − 8.67°, − 5.13°) and distal (4.90°; 95% CI, 3.75°, 6.04°) inclination of the maxillary first molars were observed in the DL group, compared to the other two groups. The mesial movement (− 0.40 mm; 95% CI, − 0.83 mm, − 0.03 mm) of the maxillary first molars was found in the IL group, while the DL (0.44 mm; 95% CI, 0.15 mm, 0.73 mm) and IL (0.62 mm; 95% CI, 0.28 mm, 0.96 mm) groups exhibited distal movement. In the DH group, the lingual inclination changes of maxillary central incisor (5.04°; 95% CI, 2.82°, 7.26°) were significantly lower, which is indicative of good lingual root torque control of the maxillary anterior teeth.

Conclusion
Three miniscrew anchorage systems produced significantly different 3-D maxillary tooth movement. The maxillary first molars were significantly buccally and distally inclined and intruded in patients using direct miniscrew anchorages with low crimpable hooks. Direct miniscrew anchorages with high crimpable hooks could help to achieve better lingual root torque control of the maxillary incisors.
Trial registration The trial was registered at www.chictr.org.cn (ChiCTR1900026960). Registered 27 October 2019.
Petrophysical characterization, BIB-SEM imaging, and permeability models of tight carbonates from the Upper Jurassic (Malm ß), SE Germany
Authors: Freitag Simon , Klaver Jop , Malai Iulian S. , Klitzsch Norbert , Urai Janos L. , Stollhofen Harald , Bauer Wolfgang , Schmatz Joyce 
Published: December 2022

AbstractTight carbonate rocks are important hydrocarbon and potential geothermal reservoirs, for example, in CO2-Enhanced Geothermal Systems. We report a study of outcrop samples of tectonically undeformed tight carbonates from the upper Jurassic “Malm ß” formation in Southern Germany near the town of Simmelsdorf (38 km NE of Nuremberg) to understand bulk petrophysical properties in relation to microstructure and to compare models for permeability prediction in these samples. We applied Archimedes isopropanol immersion, Helium pycnometry, mercury injection, gamma density core logging, and gas permeability measurements, combined with microstructural investigations and liquid metal injection (LMI-BIB-SEM). In addition, ultrasonic velocity was measured to allow geomechanical comparison of stratigraphically equivalent rocks in the South German Molasse Basin (SGMB). Results show only small variations, showing that the formation is rather homogeneous with bulk porosities below 5% and argon permeabilities around 1.4E−17 m2. The presence of stylolites in some of the samples has neither a significant effect on porosity nor permeability. Pores are of submicron size with pore throats around 10 nm and connected as shown by Mercury injection and Liquid Metal injection. Samples have high dynamic Young’s Modulus of 73 ± 5 GPa as expected for lithified and diagenetically overmature limestones. Moreover, no trends in properties were observable toward the faults at meter scale, suggesting that faulting was post-diagenetic and that the matrix permeabilities were too low for intensive post-diagenetic fluid–rock interaction. Petrophysical properties are very close to those measured in the SGMB, illustrating the widespread homogeneity of these rocks and justifying the quarry as a reasonable reservoir analog. Permeability prediction models, such as the percolation theory-based Katz-Thompson Model, Poiseuille-based models, like the Winland, the Dastidar, the capillary tube, and the Kozeny-Carman Models, as well as several empirical models, namely, the Bohnsack, the Saki, and the GPPT Models, were applied. It is shown that the capillary tube Model and the Saki Model are best suited for permeability predictions from BIB-SEM and mercury injection capillary pressure results, respectively, providing a method to estimate permeability in the subsurface from drill cuttings. Matrix permeability is primarily controlled by the pore (throat) diameters rather than by the effective porosity.
An optimized biofumigant improves pepper yield without exerting detrimental effects on soil microbial diversity
Authors: Tagele Setu Bazie , Kim Ryeong-Hui , Jeong Minsoo , Jung Da-Ryung , Lee Dokyung , Shin Jae-Ho 
Published: December 2022

Abstract
Background
Biofumigation is a non-chemical sustainable approach that reshapes soil microbiota to overcome challenges in way of continuous cultivation. However, the type and quantity of substrate have a significant impact on microbiota shifts and the subsequent success of biofumigation. Moreover, studies on the effects of biofumigant concentration in combination with fumigation duration on soil microbiota dynamics are very rare.

Research methods
We performed microcosm experiments to investigate how a biofumigant (Korean canola cultivar, HanRa) at various concentrations (0.5%, 1%, 2–4% w/w: biofumigant/soil) and fumigation periods (2–4 weeks) affects the soil bacterial and fungal communities. Subsequently, pot experiments employing two Korean canola cultivars (HanRa and YongSan) at 1% (w/w) were carried out.

Results
Illumina MiSeq analysis revealed that 2–4% biofumigant, regardless of incubation period, had a significant negative impact on microbial diversity and network complexity. In contrast, 1% biofumigant transformed the bacterial, fungal, and inter-kingdom networks into a highly connected and complex network without affecting microbial diversity. Bacillus, Clostridium, and Pseudomonas were the most highly stimulated bacterial genera in the biofumigated soils, whereas the abundance of Acidobacteria members was greatly reduced. The 2–4% amendments had substantially and more differentially abundant Fusarium than the 1%. Soil nutrition (e.g., pH, nitrate, ammonium, and exchangeable potassium), fruit yield, and weed suppression were enhanced in subsequent pot experiments. Of the nine soil chemical properties, phosphate and exchangeable potassium were the main factors influencing the microbial community assembly.

Conclusions
Optimized biofumigation-mediated increase in nitrate, ammonium, and potassium availability in the soil without causing any negative effects on soil microbial diversity indicates its potential as a preplant to improve crop productivity. This study contributes significantly to our understanding of how an optimal biofumigant can help ameliorate obstacles in continuous cropping.

Graphical Abstract