bluuebunny/crossref_metadata_2025_split huggingface dataset:
Warming-induced changes of broccoli head to cauliflower-like curd in Brassica oleracea are regulated by DNA methylation as revealed by methylome and transcriptome co-profiling
Authors: Yao Zilei , Yuan Lu , Liu Ke , Wang Tingjin , Liu Bin , Zhao Yan , Gan Susheng , Chen Liping 
Published: December 2022

AbstractIncreasingly warming temperature impacts on all aspects of growth and development in plants. Flower development is a complex process that is very sensitive to ambient temperature, and warming temperatures often lead to abnormal flower development and remarkably reduce the quality and yield of inflorescent vegetables and many other crops, which can be exemplified by Brassica oleracea cv. Green Harmony F1, a broccoli cultivar, whose floral development is ceased at inflorescence meristem (at 28 °C) or floral primordium stage (at 22 °C), forming a cauliflower-like curd (28 °C) or intermediate curd (22 °C) instead of normal broccoli head at 16 °C. However, the underlying molecular regulatory mechanisms are not well understood. Here we report that warming temperature (28 °C or 22 °C) induced hypermethylation of the genome, especially the promoter regions of such sets of genes as ribosome biogenesis-related and others, leading to the suppression of the apex-highly-expressed distinctive genes, subsequently resulting in the abnormal floral development, as revealed by methylome and transcriptome co-profiling. The regulation of warming-induced abnormal floral development in broccoli was further verified by the fact that the DNA methylation inhibitor 5-azacytidine (5-azaC) released the expression of genes from the warming temperature-induced suppression, and restored the broccoli development to normalcy at warming temperature. The research provided new approaches to breeding broccoli and other crops for growing in wider or warmer temperature zones.
EDIFY (Eating Disorders: Delineating Illness and Recovery Trajectories to Inform Personalised Prevention and Early Intervention in Young People): project outline
Authors: Hemmings Amelia , Sharpe Helen , Allen Karina , Bartel Heike , Campbell Iain C. , Desrivières Sylvane , Dobson Richard J.B. , Folarin Amos A. , French Tara , Kelly Jonathan , Micali Nadia , Raman Sneha , Treasure Janet , Abbas Ruby , Heslop Beck , Street Tallulah , Schmidt Ulrike 
Published: December 2022

SummaryEDIFY (Eating Disorders: Delineating Illness and Recovery Trajectories to Inform Personalised Prevention and Early Intervention in Young People) is an ambitious research project aiming to revolutionise how eating disorders are perceived, prevented and treated. Six integrated workstreams will address key questions, including: What are young people's experiences of eating disorders and recovery? What are the unique and shared risk factors in different groups? What helps or hinders recovery? How do the brain and behaviour change from early- to later-stage illness? How can we intervene earlier, quicker and in a more personalised way? This 4-year project, involving over 1000 participants, integrates arts, design and humanities with advanced neurobiological, psychosocial and bioinformatics approaches. Young people with lived experience of eating disorders are at the heart of EDIFY, serving as advisors and co-producers throughout. Ultimately, this work will expand public and professional perceptions of eating disorders, uplift under-represented voices and stimulate much-needed advances in policy and practice.
Amplitude-dependent rheological responses of axisymmetric grains
Authors: Amereh M. , Nadler B. 
Published: December 2022

Abstract
Oscillatory shear flows of axisymmetric grains exhibit amplitude-dependent rheological responses, which is related to the evolution of the microstructure. In this work, it is shown that the highly ordered configuration of grains at steady-state shear flow undergoes microstructural rearrangement when subjected to shear oscillations. This rearrangement may lead to reduced ordering configurations which give rise to macroscale shear hardening, which can result in shear jamming if the applied shear traction is below the critical shear resistance. On the other hand, it was observed that applying oscillatory shear to the primary condensed shear flow enhances flowability due to microstructure rearrangement. In this study, we investigate the amplitude-dependent rheological responses of axisymmetric grains subjected oscillatory shear flows. First, we look into the evolution of grains alignment subjected to a range of oscillation amplitudes, where we show that the lower oscillation amplitudes have the potential to change the orientation from the ordered steady state to a completely disordered (isotropic) orientation. Next, we study the dependency of the shear flow resistance on the microstructure configuration, and show that the strain hardening and potential jamming have strong dependency on the oscillations amplitude. We also show that, in the case of jamming, the shear strain and the corresponding number of oscillation cycles depend not only on the grains aspect ratio but also on the oscillation amplitude.
Does the Risk of Embryo Abnormality Increase in PCOS Women? A Secondary Analysis of a Multicenter, Randomized Controlled Trial
Authors: Wang Jiahui , Zhou Wei , Song Zhiyi , Ni Tianxiang , Zhang Qian , Chen Zi-Jiang , Yan Junhao 
Published: December 2022

Abstract

Context
Some studies have reported the early miscarriage rate is higher in polycystic ovary syndrome (PCOS) women. However, there is a lack of evidence as to whether the risk of embryo abnormalities increases in PCOS women.

Objective
This work aimed to evaluate the association between PCOS and embryo ploidy.

Methods
A secondary analysis of a multicenter, randomized controlled trial was conducted from July 2017 to June 2018. The original intent was to identify whether preimplantation genetic test for aneuploidy (PGT-A) improves the live birth rate as compared with in vitro fertilization (IVF). From 14 reproductive centers, 190 patients diagnosed with PCOS and 1:1 age-matched non-PCOS patients were chosen from a PGT-A group. A total of 380 patients with 1118 embryos were included in our study. Intervention included women diagnosed with PCOS, and the main outcome measures were embryonic aneuploidy and embryonic mosaic.

Results
After adjusting for potential confounders, the rate of embryonic aneuploidy and embryonic mosaic in the PCOS group were comparable with the control group (embryonic aneuploid rate PCOS group: 14.0% vs control group: 18.3%, adjusted OR [95% CI]: 0.78 [0.54, 1.12]; P = .19; embryonic mosaic rate 10.9% vs 10.1%, adjusted OR [95% CI]: 0.91 [0.59, 1.40]; P = .66). We further stratified PCOS women into 4 groups according to phenotype. The rate of aneuploid and mosaic embryos was comparable between each PCOS phenotype and control group. There was still no significant difference of embryonic aneuploid and embryo mosaic rates among the 4 phenotypes.

Conclusion
The risk of aneuploid and mosaic embryos did not increase in PCOS women. Thus, we suggest that the miscarriage rate arising from abnormal embryonic chromosomes could be similar between PCOS and non-PCOS women.
Difference of Risk of Pancreatic Cancer in New-Onset Diabetes and Long-standing Diabetes: A Population-based Cohort Study
Authors: Lee Hee Seung , Chae Wonjeong , Sung Min Je , Keum Jiyoung , Jo Jung Hyun , Chung Moon Jae , Park Jeong Youp , Park Seung Woo , Song Si Young , Park Eun-Cheol , Nam Chung Mo , Jang Sung-In , Bang Seungmin 
Published: December 2022

Abstract

Context
Considering the absence of methods to find pancreatic cancer early, surveillance of high-risk groups is needed for early diagnosis.

Objective
The study aimed to investigate the effect in the incidence of pancreatic cancer and the differences between new-onset diabetes mellitus (NODM) and long-standing DM (LSDM) since NODM group is a representative high-risk group.

Methods
The Korean National Health Insurance Service–National Sample Cohort between 2002 and 2013 data were used. Regarding 88 396 people with DM (case group), we conducted a 1:1 propensity score matching to select a matched non-DM population (control group). To investigate the interaction between DM and the time variable distinguishing NODM and LSDM, we performed a multivariate time-dependent Cox regression analysis.

Results
The incidence of pancreatic cancer was higher in the DM group compared to the non-DM group (0.52% vs 0.16%; P &lt; .001). The DM group had shown different risk of pancreatic cancer development according to the duration since the DM diagnosis (NODM hazard ratio (HR): 3.81; 95% CI, 2.97-4.88; P &lt; .001; LSDM HR: 1.53; 95% CI, 1.11-2.11; P &lt; .001). When the NODM and the LSDM groups were compared, the risk of pancreatic cancer was higher in the NODM group than in the LSDM group (HR: 1.55; P = .020). In subgroup analysis, NODM group showed that men (HR = 4.42; 95% CI, 3.15-6.19; P &lt; .001) and patients who were in their 50 seconds (HR = 7.54; 95% CI, 3.24-17.56; P &lt; .001) were at a higher risk of developing pancreatic cancer than matched same sex or age control group (non-DM population), respectively.

Conclusion
The risk of pancreatic cancer was greater in people with DM than in a non-DM population. Among people with DM, NODM showed a higher risk of pancreatic cancer than LSDM.
Approach to the Patient: Management of Parathyroid Diseases Across Pregnancy
Authors: Appelman-Dijkstra Natasha M , Pilz Stefan 
Published: December 2022

Abstract
Taking care of patients with parathyroid disorders during pregnancy requires consideration of the physiological fundamental changes in bone and mineral metabolism occurring in these women. Diagnostic and therapeutic procedures regarding primary hyperparathyroidism (PHPT) and hypoparathyroidism significantly differ from the nonpregnant population. PHPT should preferably be cured by parathyroidectomy before pregnancy since in women with hypercalcemic PHPT, maternal and fetal pregnancy complications seem to increase according to the degree of hypercalcemia. Parathyroidectomy, if needed during pregnancy, is preferentially performed in the second trimester. Conservative treatment is recommended for milder cases and is mainly restricted to hydration, with only limited evidence regarding drug treatment. Women with hypoparathyroidism can be informed that there are no major concerns regarding disease-associated infertility and that the risk of pregnancy complications is low if the disease is properly managed. Regular active surveillance is recommended, as requirements for calcium and active vitamin D may change during the course of pregnancy in either direction, with an overall trend for rather reduced doses. Any woman suffering from parathyroid disorders during pregnancy requires further surveillance in the postpartum period and during lactation, as there is an increased risk of hypercalcemia after delivery. Newborns of mothers with parathyroid diseases should, depending on disease severity, be carefully monitored for calcium levels in the first days (to weeks) after delivery since intrauterine exposure to hyper- or hypocalcemia may impact their postnatal regulation of calcium metabolism.
Sex-specific Trajectories of Insulin Resistance Markers and Reduced Renal Function During 18 Years of Follow-up: TLGS
Authors: Amouzegar Atieh , Honarvar Mohammadjavad , Masoumi Safdar , Tohidi Maryam , Mehran Ladan , Azizi Fereidoun 
Published: December 2022

Abstract

Context
The evidence suggest that insulin resistance (IR) complicates chronic kidney disease (CKD); however, the longitudinal association of IR with development of CKD is unknown.

Objective
This work aimed to investigate the association between the dynamic course of insulin resistance and CKD.

Methods
In the longitudinal, population-based Tehran Lipid and Glucose Study, 3071 eligible participants aged 20 years or older were followed for 18 years at 3-year intervals. Homeostatic model assessment of insulin resistance (HOMA-IR) and clinical surrogate markers of IR, including triglyceride-glucose index (TyG), visceral adiposity index (VAI), and lipid accumulation product (LAP), were calculated. Using latent variable mixture modeling, sex-specific trajectories were plotted for each IR marker. Trajectory group association of the IR markers with CKD was determined using the multivariable Cox proportional-hazards regression model.

Results
For HOMA-IR, 2 distinct trajectory patterns (stable and increasing), and for TyG, VAI, and LAP, 3 trajectories (low, moderate, and high) were identified. The participants with an increasing HOMA-IR trajectory had a significantly increased risk of CKD in men (hazard ratio [HR]: 1.72; 95% CI, 1.06-2.79) and women (HR: 1.37; 95% CI, 1.00-1.89) after adjusting for confounding variables. The high TyG and VAI trajectory classes were associated with a higher risk of CKD than the low TyG and VAI trajectory classes both in men (TyG: HR: 1.97; 95% CI, 1.12-3.46; VAI: HR:1.66; 95% CI, 1.06-2.62) and women (TyG: HR: 1.50; 95% CI, 1.06-2.12; VAI: HR:1.66; 95% CI, 1.20-2.31). In contrast, the high LAP (HR: 3.38; 95% CI, 2.08-5.48) trajectory was associated with incident CKD only in women.

Conclusion
An increasing trend of HOMA-IR is associated with a higher risk of CKD in men and women. Among clinical IR surrogate markers, abnormal trajectory patterns of LAP in women and TyG and VAI in both sexes are associated with a higher risk of CKD.
Novel Insulin-Like Growth Factor 1 Gene Mutation: Broadening of the Phenotype and Implications for Insulin Resistance
Authors: Giacomozzi Claudio , Martin Ayelen , Fernández María Celia , Gutiérrez Mariana , Iascone Maria , Domené Horacio M , Dominici Fernando P , Bergadá Ignacio , Cangiano Biagio , Persani Luca , Pennisi Patricia A 
Published: December 2022

Abstract

Context
Insulin-like growth factor (IGF)1 gene mutations are extremely rare causes of pre- and postnatal growth retardation. Phenotype can be heterogenous with varying degrees of neurosensory deafness, cognitive defects, glucose metabolism impairment and short stature.

Objective
This study describes a 12.6-year-old girl presenting with severe short stature and insulin resistance, but with normal hearing and neurological development at the lower limit of normal.

Methods
DNA was obtained from the proband and both parents for whole exome sequencing (WES). In silico analysis was performed to predict the impact of the IGF1 variant on IGF1 and insulin receptors (IGF1R and IR) signaling. Phosphorylation of the IGF1R at activating Tyr residues and cell proliferation analyses were used to assess the ability of each subject's IGF1 to bind and activate IGF1R.

Results
The proband had low immunoreactive IGF1 in serum and WES revealed a novel homozygous IGF1 missense variant (c.247A&gt;T), causing a change of serine 83 for cysteine (p.Ser83Cys; p.Ser35Cys in mature peptide). The proband's parents were heterozygous for this mutation. In silico analyses indicated the pathogenic potential of the variant with electrostatic variations with the potential of hampering the interaction with the IGF1R but strengthening the binding to IR. The mutant IGF1 protein had a significantly reduced activity on in vitro bioassays.

Conclusion
We describe a novel IGF1 mutation leading to severe loss of circulating IGF1 immunoreactivity and bioactivity. In silico modeling predicts that the mutant IGF1 could interfere with IR signaling, providing a possible explanation for the severe insulin resistance observed in the patient. The absence of significant hearing and neurodevelopmental involvement in the present case is unusual and broadens the clinical spectrum of IGF1 mutations.
Aggressive Subtypes of Papillary Thyroid Carcinoma Smaller Than 1 cm
Authors: Lee Jin Seok , Lee Jun Sung , Yun Hyeok Jun , Kim Seok Mo , Chang Hojin , Lee Yong Sang , Chang Hang-Seok , Park Cheong Soo 
Published: December 2022

AbstractContextTumor size is important in determining the range of surgery in papillary thyroid carcinomas (PTCs), especially those smaller than 1 cm.ObjectiveWe aimed to analyze the features of small PTCs with aggressive subtypes based on histological characteristics.MethodsIn this retrospective study, we reviewed the medical records of 11 570 patients with PTCs smaller than or equal to 1 cm who underwent thyroidectomy between January 2009 and December 2016. Aggressive subtypes included diffuse sclerosing, solid, tall cell, columnar cell, and hobnail subtypes.ResultsAmong the 11 570 patients with PTCs smaller than or equal to 1 cm, 177 aggressive PTC subtypes were identified. Propensity score matching revealed 110 tumors (62.1%) with extrathyroidal extension of aggressive PTC subtypes and 451 (51.1%) nonaggressive PTC subtypes (95% CI, 0.41-0.80; P &lt; .001). Metastatic central and lateral neck lymph nodes constituted 3.06 ± 3.67 and 3.81 ± 5.39 of aggressive PTC subtypes and 1.22 ± 2.14 and 2.85 ± 3.79 of nonaggressive PTC subtypes, respectively (central neck nodes: 95% CI, 1.42-2.26; P &lt; .001; lateral neck nodes: 95% CI, 2.9-5.90; P &lt; .001). Seven patients with aggressive PTC subtypes (3.95%) and 12 with nonaggressive PTC subtypes (1.7%) exhibited recurrence.ConclusionAggressive subtypes of small PTC tumors smaller than or equal to 1 cm exhibited more extrathyroidal extension and neck node metastasis. This study suggests that surgeons should consider the aggressive subtypes as important factors when deciding the range of surgery in PTCs smaller than 1 cm.
Evolution of Nrf2 Gene Expression in HIT-T15 β-Cells During Chronic Oxidative Stress and Glucose Toxicity
Authors: Abebe Tsehay , Bogachus Lindsey , Vegaraju Adithya Krishna , Robertson R Paul 
Published: December 2022

Abstract

Context
Chronic exposure of pancreatic islets to elevated glucose levels causes progressive declines in beta cell Pdx-1 and insulin gene expression, and glucose-induced insulin secretion. This has been shown to be associated with excessive islet reactive oxygen species and consequent damage to beta cell function, a process termed glucose toxicity. In short-term rodent in vivo studies, Nrf2 (Kelch-like ECH-associated protein 1:nuclear factor erythroid-derived-2 related factor complex) has been shown to play a central role in defending beta cells from oxidative damage via activation of antioxidant gene expression.

Objective
The current studies were primarily designed to examine the behavior of Nrf2 gene expression during longer term exposure of beta cells to glucose toxicity.

Methods and Results
We provide evidence that gene expression of Nrf2 in HIT-T15 cells, an insulin-secreting beta-cell line, undergoes a biphasic response characterized by an initial decrease followed by increased expression during prolonged culturing of these cells in a physiologic (0.8 mM) but not a supraphysiologic (16.0 mM) glucose concentration. This was associated with a slight rise in HO-1 gene expression. Pdx-1 and insulin mRNA levels also decreased but then stabilized in late passages of cells that had been cultured in low glucose concentrations.

Conclusion
These complex events support the concept that Nrf2 gene expression plays an important regulatory role in defending beta cells during prolonged exposure to oxidative stress.
Predictors of unwillingness or inaccessibility to receive the COVID-19 vaccination among persons with disabilities in Bangladesh
Authors: Hossain Md Zahid , Hossain Md Akter , Al Ansary Mohammad Yaqub , Raigangar Veena , Rahman Md Habibur , Akter Ruksana , Ahmed Shameem , Jahan Sharmila , Jahid Iqbal Kabir , Hossain K. M. Amran 
Published: December 2022

Introduction Persons with disabilities (PWDs) are among the most vulnerable communities to suffer the serious consequences of COVID-19, and accepting COVID-19 vaccination is one of the recommended health advisories for them. Unwillingness to receive vaccines is a concerning issue, especially in the countries of Southeast Asia. The study aims to find out the COVID-19 vaccination rate of persons with disabilities (PWDs) in Bangladesh, the rate of unwillingness or inaccessibility of vaccination for PWDs, and predict the possible reasons for unwillingness. Methods A descriptive cross-sectional survey of PWDs aged 12 years or more was conducted in 12 rehabilitation centers in Bangladesh between February 2022 and May 2022. A self-developed structured questionnaire on socio-demographic, disability, and health indicators, acceptance and accessibility of COVID-19 vaccines, and knowledge and attitude towards vaccination was used for the survey. The study has been conducted according to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines for a cross-sectional survey. Results With a 69% response rate, 241 PWDs participated in the study. PWDs with a spinal cord injury were the major respondents (62.7%). A total of 186 (77.2%) PWDs had taken one or more doses of COVID-19 vaccines of three doses supplied, and 55 (22.8%) were unwilling to have a vaccination. All vaccine recipients reported good accessibility to vaccination booths. The predictive factors of unwillingness were spinal cord injury type of disability (OR .36, P<.01), people coming to the rehabilitation center from rural areas (OR .44, P<.01), poor knowledge of COVID-19 vaccination (OR .78, P<.01), and dependency on mobility (OR.24, P<.001). Conclusion More than one-fifth of the persons with disabilities aged between 12 and 80 years were unwilling to receive the COVID-19 vaccination despite the accessibility of information and availability of the COVID-19 vaccine, mainly due to poor knowledge and mobility issues.
Reduction of mineralizing activity of periodontal bone tissue in rats with consumption of ordinary sunflower oil
Authors: Levitsky A. , Pupin T. , Selivanskaya I. , Lapinskaya A. , Markov A. 
Published: December 2022

More than 20 % of people over the age of 50 suffer from osteoporosis. Lipids play an important role in the pathogenesis of osteoporosis.
Background. To investigate the effect of ordinary (high-linoleic) sunflower oil (OSO) on the state of periodontal bone tissue.
Methods. In 5 series of experiments, determine the mineralization activity of the alveolar appendix of the mandible rats that were obtained with feed 5 or 15 percent of OSO for from 22 to 75 days. Mineralizing activity was determined by the ratio of activity of alkaline and acid phosphatase. In liver lipids (fractions of phospholipids and free fatty acids) were determined by the content of long-chain polyunsaturated fatty acids (LCPUFA) by the gas chromatographic method.
Results. A decrease in mineralizing activity in OSO consumption, especially when using a feed with 15 % of OSO against a dysbiosis or metabolic syndrome. A significant (almost 10 times) is shown to reduce the content of ω-3 PUFA in fraction of phospholipids of the liver of rats receiving a diet with a content of OSO.
Conclusion. Sunflower oil reduces the mineralizing activity of periodontal bone tissue by reducing the endogenous biosynthesis of ω-3 PUFA.
CONTRIBUTO DA TEORIA RICOEURIANA DA TRÍPLICE MIMESE PARA UMA CONCEPÇÃO SEMÂNTICA DA IMAGINACÃO
Authors: CUNHA WANDERLEY 
Published: December 2022

Temps et Récit é uma obra imprescindível para a compreensão do projeto filosófico de Paul Ricoeur, dado que ela funciona indubitavelmente como uma espécie de dobradiça no conjunto da produção intelectual desenvolvida pelo filósofo francês, pois, por um lado, através da noção de ‘inovação semântica’, se articula à perspectiva poética desenvolvida em La Méthaphore vive e, por outro, por meio da noção de ‘identidade narrativa’ se articula à perspectiva prática desenvolvida em Soi-meme comme un autre. Assim sendo, este artigo se propõe a efetuar uma introdução à leitura de Temps et Récit a partir de sua articulação com a concepção semântica da imaginação elaborada por Ricoeur em seus estudos sobre os enunciados metafóricos. Para tanto, empreende-se uma análise do modelo de articulação entre a experiência aporética do tempo e a inteligibilidade narrativa que Ricoeur denomina de ‘tríplice mimese’, com o intuito de se evidenciar as especificidades do funcionamento da imaginação semântica no domínio da narrativa.