bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential target multiplexed spinal cord stimulation using a paddle-type lead placed at the appropriate site for neuropathic pain after spinal cord injury in patients with past spinal surgical histories: study protocol for an exploratory clinical trial
Authors: Tanei Takafumi , Maesawa Satoshi , Nishimura Yusuke , Nagashima Yoshitaka , Ishizaki Tomotaka , Ando Masahiko , Kuwatsuka Yachiyo , Hashizume Atsushi , Kurasawa Shimon , Saito Ryuta 
Published: June 2023

Abstract
Background
Neuropathic pain after spinal cord injury (SCI), both traumatic and non-traumatic, is refractory to various treatments. Spinal cord stimulation (SCS) is one of the neuromodulation therapies for neuropathic pain, although SCS has insufficient efficacy for neuropathic pain after SCI. The reasons are presumed to be inappropriate locations of SCS leads and conventional tonic stimulation itself does not have a sufficient analgesic effect for the pain. In patients with past spinal surgical histories, the cylinder-type leads are likely to be placed on the caudal side of the SCI because of surgical adhesions. Differential target multiplexed (DTM) stimulation is one of the latest new stimulation patterns that is superior to conventional stimulation.

Methods
A single-center, open-label, randomized, two-way crossover trial is planned to investigate the efficacy of SCS using DTM stimulation placing a paddle lead at the appropriate site for neuropathic pain after SCI in patients with spinal surgical histories. The paddle-type lead delivers energy more efficiently than a cylinder-type lead. This study consists of two steps: SCS trial (first step) and SCS system implantation (second step). The primary outcome is rates of achieving pain improvement with more than 33% reduction 3 months after SCS system implantation. The secondary outcomes are to be evaluated as follows: (1) effectiveness of DTM and tonic stimulations during the SCS trial; (2) changes of assessment items from 1 to 24 months; (3) relationships between the result of the SCS trial and the effects 3 months after SCS system implantation; (4) preoperative factors associated with a long-term effect, defined as continuing for more than 12 months; and (5) whether gait function improves from 1 to 24 months.

Discussion
A paddle-type lead placed on the rostral side of SCI and using DTM stimulation may provide significant pain relief for patients with intractable neuropathic pain after SCI in patients with past spinal surgical histories.

Trial registration
Japan Registry of Clinical Trials (jRCT) jRCT 1042220093. Registered on 21 November 2022, and last modified on 6 January 2023. jRCT is approved as a member of the Primary Registry Network of WHO ICTRP.
Three R2R3-MYB transcription factors from banana (Musa acuminata) activate structural anthocyanin biosynthesis genes as part of an MBW complex
Authors: Busche Mareike , Pucker Boas , Weisshaar Bernd , Stracke Ralf 
Published: June 2023

Abstract
Objective
Bananas are one of the most popular fruits in the world, providing food security and employment opportunities in several developing countries. Increasing the anthocyanin content of banana fruit could improve the health-promoting properties. Anthocyanin biosynthesis is largely regulated at the transcriptional level. However, relatively little is known about the transcriptional activation of anthocyanin biosynthesis in banana.

Results
We analysed the regulatory activity of three Musa acuminata MYBs that were predicted by bioinformatic analysis to transcriptionally regulate anthocyanin biosynthesis in banana. MaMYBA1, MaMYBA2 and MaMYBPA2 did not complement the anthocyanin-deficient phenotype of the Arabidopsis thaliana pap1/pap2 mutant. However, co-transfection experiments in A. thaliana protoplasts showed that MaMYBA1, MaMYBA2 and MaMYBPA2 function as components of a transcription factor complex with a bHLH and WD40 protein, the so called MBW complex, resulting in the activation of the A. thaliana ANTHOCYANIDIN SYNTHASE and DIHYDROFLAVONOL 4-REDUCTASE promoters. The activation potential of MaMYBA1, MaMYBA2 and MaMYBPA2 was increased when combined with the monocot Zea mays bHLH ZmR instead of the dicot AtEGL3. This work paves the path towards decoding the MBW complex-mediated transcriptional activation of anthocyanin biosynthesis in banana. It will also facilitate research towards increased anthocyanin content in banana and other monocot crops.
Recurrent diabetic myonecrosis in an African American woman with long-standing uncontrolled type 2 diabetes: a case report
Authors: Kurup Varsha , Nagoshi Ying , Rivera-Zengotita Marie , Maska Edlira 
Published: June 2023

Abstract
Background
Diabetic myonecrosis, also called diabetic muscle infarction, is an uncommon complication of uncontrolled diabetes mellitus and is frequently underdiagnosed. The objective of this case report is to highlight the challenges in the early diagnosis and treatment of this disease.

Case presentation
A 51-year-old African American woman with a long history of uncontrolled diabetes mellitus presented to her primary care physician with right thigh pain. A diagnosis of diabetes myonecrosis was made on the basis of magnetic resonance imaging, biopsy, and negative autoimmune panel. After failing conservative treatment, the patient was treated with prednisone with gradual improvement of her symptoms. However, she had a recurrence of myonecrosis almost one year after her original presentation, which was also treated with prednisone. The recurrence had a shorter course and the patient recovered well. Challenges to the treatment in this patient were her debilitating pain and her underlying chronic kidney disease.

Conclusions
A high index of suspicion for diabetic myonecrosis is necessary when a patient with diabetes presents with unilateral focal leg pain and swelling. Magnetic resonance imaging and biopsy can help confirm the diagnosis. Prednisone may be considered in patients who lack spontaneous regression with just rest. Educating healthcare professionals about this uncommon condition is of utmost importance in avoiding unnecessary testing and inappropriate treatment.
Avelumab in metastatic collecting duct carcinoma of the kidney: a case report
Authors: Pyrgidis Nikolaos , Sokolakis Ioannis , Haltmair Gena , Heller Vitus , Hatzichristodoulou Georgios 
Published: June 2023

Abstract
Background
Collecting duct carcinoma (CDC) of the kidney is associated with an aggressive course, limited response to treatment, and poor prognosis. Platinum-based chemotherapy is currently recommended as the first-line treatment in patients with metastatic CDC. Accumulating evidence supports the use of immunotherapy with checkpoint inhibitors as second-line therapy.

Case presentation
In this case report, we describe the first case of avelumab administration due to disease progression during chemotherapy with gemcitabine and cisplatin in a Caucasian, 71-year-old man presenting with multiple metastases due to CDC of the kidney. The patient initially responded well to four cycles of chemotherapy and his performance status improved. After two additional chemotherapy cycles, the patient presented with new bone and liver metastases (mixed response to chemotherapy with an overall 6-month progression-free survival). We offered him avelumab as a second-line treatment in this setting. The patient received a total of three cycles of avelumab. The disease remained stable (no new metastases during treatment with avelumab), and the patient developed no complications. To alleviate his symptoms, radiation therapy for the bone metastases was decided. Despite successful radiation of the bone lesions and further improvement of symptoms, the patient developed hospital-acquired pneumonia and died approximately ten months after the initial diagnosis of CDC.

Conclusions
Our findings suggest that the applied treatment modality with gemcitabine and cisplatin chemotherapy followed by avelumab was effective in terms of both progression-free survival and quality of life. Still, further studies assessing the use of avelumab in this setting are mandatory.
Clinical prognosis and related molecular features of hepatitis B-associated adolescent and young adult hepatocellular carcinoma
Authors: Lv Tao , Zhang Bo , Xu Xi , Jiang Chenhao , Zheng Daofeng , He Diao , Zhou Yongjie , Yang Jiayin 
Published: June 2023

Abstract
Background
Inattention has been given to the pathogenesis of adolescent and young adult (AYA) hepatocellular carcinoma (HCC). Due to the more advanced tumor progression and poorer prognosis of AYA-HCC, together with a better tolerance ability, noncirrhotic background, and a stronger willingness to treat AYA-HCC, clinical and molecular biology studies are urgent and necessary, especially for those with hepatitis B infection.

Methods
For clinical aspects, the overall survival, the recurrence-free survival, and the Cox analyses were performed. Then, functional analysis, gene clustering, metabolic-related analysis, immune infiltration and competing endogenous RNA (ceRNA) construction were carried out using whole transcriptome sequencing technique.

Results
Based on the clinical information of our HCC cohort, the overall survival and recurrence-free survival rates were worse in the AYA group than in the elderly group as previously described. According to our whole transcriptome sequencing results, functional analysis revealed that metabolism-related pathways as well as protein translation and endoplasmic reticulum processing were enriched. Then the hub metabolism-related genes were screened by metabolite–protein interactions (MPIs) and protein–protein interactions (PPIs). Fatty acid metabolism is a crucial component of metabolic pathways, abnormalities of which may be the reason for the worse prognosis of HBV-AYA HCC. Finally, the relationship of disrupted expression of metabolism-related genes with immune infiltration was also analyzed, and the lncRNA‒miRNA‒mRNA-related ceRNA network for HBV-AYA HCC was constructed, which may provide new cues for HBV-AHA HCC prevention.

Conclusion
The worse prognosis and recurrence rate of HBV-AYA HCC may be related to abnormalities in metabolism-related pathways, especially disorders of fatty acid metabolism.
The therapeutic potential of purified cannabidiol
Authors: O’Sullivan Saoirse Elizabeth , Jensen Sanne Skov , Nikolajsen Gitte Nykjaer , Bruun Heidi Ziegler , Bhuller Rhenu , Hoeng Julia 
Published: June 2023

AbstractThe use of cannabidiol (CBD) for therapeutic purposes is receiving considerable attention, with speculation that CBD can be useful in a wide range of conditions. Only one product, a purified form of plant-derived CBD in solution (Epidiolex), is approved for the treatment of seizures in patients with Lennox-Gastaut syndrome, Dravet syndrome, or tuberous sclerosis complex. Appraisal of the therapeutic evidence base for CBD is complicated by the fact that CBD products sometimes have additional phytochemicals (like tetrahydrocannabinol (THC)) present, which can make the identification of the active pharmaceutical ingredient (API) in positive studies difficult. The aim of the present review is to critically review clinical studies using purified CBD products only, in order to establish the upcoming indications for which purified CBD might be beneficial. The areas in which there is the most clinical evidence to support the use of CBD are in the treatment of anxiety (positive data in 7 uncontrolled studies and 17 randomised controlled trials (RCTs)), psychosis and schizophrenia (positive data in 1 uncontrolled study and 8 RCTs), PTSD (positive data in 2 uncontrolled studies and 4 RCTs) and substance abuse (positive data in 2 uncontrolled studies and 3 RCTs). Seven uncontrolled studies support the use of CBD to improve sleep quality, but this has only been verified in one small RCT. Limited evidence supports the use of CBD for the treatment of Parkinson’s (3 positive uncontrolled studies and 2 positive RCTs), autism (3 positive RCTs), smoking cessation (2 positive RCTs), graft-versus-host disease and intestinal permeability (1 positive RCT each). Current RCT evidence does not support the use of purified oral CBD in pain (at least as an acute analgesic) or for the treatment of COVID symptoms, cancer, Huntington’s or type 2 diabetes. In conclusion, published clinical evidence does support the use of purified CBD in multiple indications beyond epilepsy. However, the evidence base is limited by the number of trials only investigating the acute effects of CBD, testing CBD in healthy volunteers, or in very small patient numbers. Large confirmatory phase 3 trials are required in all indications.
3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae
Authors: Yan Mingfeng , Yu Yonghong , Luo Lizhen , Huang Mei , Zhang Yuanyin , Su Jingtong , Zhang Wenbin , Ma Jincheng , Hu Zhe , Wang Haihong 
Published: June 2023

AbstractThe 3-ketoacyl-ACP synthase III (FabH), a key enzyme for bacteria growth, catalyses the last step of the initiation of bacterial fatty acid synthesis. Rice bacterial blight is caused by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), which is widely studied as a model bacterium. Bioinformatics analysis showed that the X. oryzae pv. oryzae PXO99A genome encodes three FabH homologous proteins with unknown functions. In this study, we found that only PXO_02706 (fabH1) encodes a functional FabH, the key enzyme in the production of branched-chain fatty acid, which is essential for the branched-chain diffusible signal factor family signals in Xoo. Interestingly, we found that FabH1 is not essential for fatty acid biosynthesis in Xoo. Pathogenicity analysis showed that loss of fabH1 caused a significant decrease in virulence of Xoo. Genetic and phenotypic analyses revealed that fabH1 plays a key role in multiple Xoo virulence-related activities, including exopolysaccharide (EPS) production, biofilm formation, motility, and resistance to environmental stresses.
Vacuum-assisted closure therapy for an empyema with bronchopleural fistula with artificial dermis covering the bronchial stump
Authors: Matsumoto Akira , Shoji Tsuyoshi , Katakura Hiromichi 
Published: June 2023

Abstract
Background
Vacuum-assisted closure (VAC) therapy has recently been reported to be useful for treating empyema complicated by bronchopleural fistula (BPF). Since the pleural cavity must be closed to apply this treatment, various fistula closure methods for the fistula have been used in previous reports. We report a case in which VAC therapy could be applied more safely by simply covering the fistula with an artificial dermis.

Case presentation
A 72-year-old man who underwent thoracoscopic lower lobectomy of the right lung for squamous cell carcinoma pT1cN0 developed BPF on the postoperative day 22. First, infection control was achieved by open-window thoracostomy and antibiotic therapy. Subsequently, VAC therapy was applied by covering the fistula with an artificial dermis, and closure of the bronchial stump was completely achieved. Subsequently, the thoracostomy was closed surgically.

Conclusion
Covering the fistula with an artificial dermis allowed for the introduction of VAC therapy when treating empyema complicated by BPF.
Propagation of two-wave solitons depending on phase-velocity parameters of two higher-dimensional dual-mode models in nonlinear physics
Authors: Singh Shailendra , Saha Ray Santanu 
Published: June 2023

Abstract
Nonlinear evolution equations exhibit a variety of physical behaviours, which are clearly illustrated by their exact solutions. In this view, this article concerns the study of dual-mode (2 + 1)-dimensional Kadomtsev-Petviashvili and Zakharov-Kuznetsov equations. These models describe the propagation of two-wave solitons traveling simultaneously in the same direction and with mutual interaction dependent on an embedded phase-velocity parameter. The considered nonlinear evolution equations have been solved analytically for the first time using the Paul-Painlevé approach method. As a result, new abundant analytic solutions have been derived successfully for both the considered equations. The 3D dynamics of each of the solution has been plotted by opting suitable constant values. These graphs show the dark-soliton, bright-soliton, complex dual-mode bright-soliton, complex periodic-soliton and complex dual-mode dark-soliton solutions.
Development and Validation of Quality Measures for Testosterone Prescribing
Authors: Borzecki Ann M , Conti Jennifer , Reisman Joel I , Vimalananda Varsha , Nagy Michael W , Paluri Radhika , Linsky Amy M , McCullough Megan , Bhasin Shalender , Matsumoto Alvin M , Jasuja Guneet K 
Published: June 2023

Abstract

Context
Accurate measures to assess appropriateness of testosterone prescribing are needed to improve prescribing practices.

Objective
This work aimed to develop and validate quality measures around the initiation and monitoring of testosterone prescribing.

Methods
This retrospective cohort study comprised a national cohort of male patients receiving care in the Veterans Health Administration who initiated testosterone during January or February 2020. Using laboratory data and diagnostic codes, we developed 9 initiation and 7 monitoring measures. These were based on the current Endocrine Society guidelines supplemented by expert opinion and prior work. We chose measures that could be operationalized using national VA electronic health record (EHR) data. We assessed criterion validity for these 16 measures by manual review of 142 charts. Main outcome measures included positive and negative predictive values (PPVs, NPVs), overall accuracy (OA), and Matthews Correlation Coefficients (MCCs).

Results
We found high PPVs (&gt;78%), NPVs (&gt;98%), OA (≥94%), and MCCs (&gt;0.85) for the 10 measures based on laboratory data (5 initiation and 5 monitoring). For the 6 measures relying on diagnostic codes, we similarly found high NPVs (100%) and OAs (≥98%). However, PPVs for measures of acute conditions occurring before testosterone initiation (ie, acute myocardial infarction or stroke) or new conditions occurring after initiation (ie, prostate or breast cancer) PPVs were much lower (0% to 50%) due to few or no cases.

Conclusion
We developed several valid EHR-based quality measures for assessing testosterone-prescribing practices. Deployment of these measures in health care systems can facilitate identification of quality gaps in testosterone-prescribing and improve care of men with hypogonadism.
The TRP Tutoring System in Education: System-Forming Factors
Authors: Ovchinnikov Yu. , Shpet V. 
Published: June 2023

The problem of research and justification of its relevance. Introduction. The TRP in the USSR has not only become a historical chronicle in the upbringing and inculcation of physical qualities, but the ideological basis of the spiritual and moral development of society. There is a stage of continuity of the TRP of the USSR and the TRP of Russia in an updated conceptual framework. The mechanism of implementation of TRP in Russia is being improved in accordance with the digitalization of society, the creation of online services, taking into account the development of the region, mass physical culture and sports. The introduction of a new administrative unit of a tutor-teacher of the TRP of Russia in 2014 marked new approaches to the implementation of the complex and the creation of a tutoring system. The TRP tutoring system differs from tutoring in education as a whole, therefore, it requires the allocation of system-forming factors that will help identify problems and will contribute to the development of strategic and tactical tasks for the implementation of the TRP complex, health promotion, spiritual and moral forces in social development.
The social function of a student as a tutor in TRP is presented. Students of the Kuban State University of Physical Culture, Sports and Tourism not only personally take the TRP, but also promote it through various competitions, festivals, participation in the evaluation of TRP standards in secondary schools, regional testing centers. Regional peculiarities of the tutoring system development are shown on the example of Sochi. The authors note that in 2014, with the return of the TRP, a new administrative unit tutor appears on a legal basis, which made it possible to start building a tutoring system in the TRP complex, taking into account system-forming factors. The problem of the prospects for the development of the TRP is raised, taking into account its socio-orienting function in society.
Premenstrual syndrome - theories and facts - something everyone should know
Authors: Świątek Gabriela , Środoń Maria , Sosnowska Weronika , Tkaczyk Rafał , Taracha Kalina , Tomczyk Jakub , Tomkiewicz Maciej , Brzozowska Aleksandra , Śwital Magdalena , Tokarski Michał 
Published: June 2023

Premenstrual syndrome (PMS- Premenstrual Syndrome) is a group of cyclic somatic or psychological symptoms occurring during the luteal phase of the cycle and disappearing with the onset of menstruation. The purpose of this work is to draw attention to this important but often underestimated problem, trace its pathomechanism and identify treatment methods.
The interaction of many factors and predispositions is responsible for the occurrence of PMS/PMDD (PMDD- premenstrual dysphoric disorder). One of the most important is ovulatory cycles and cyclically repeated changes in ovarian hormone secretion- fluctuations in progesterone, estradiol and prolactin levels during the various phases of the cycle. The association of emotional disturbances, severe stress and disturbances in the neurotransmitter system of the central nervous system, as well as many other factors, mentioned in the article below, is described. Various methods are used to treat PMS/PMSS, ranging from less to more invasive, including lifestyle changes, cognitive-behavioral therapy, and pharmacotherapy and surgical treatment. Currently, SSRIs-serotonin reuptake inhibitors-are considered the main therapy, as well as hormonal treatment-the two-pill.
A review of the literature shows that PMS/PMDD is a real problem that reduces quality of life and has a negative impact on family and social relationships. Increased public awareness and interest by physicians of many specialties in this problem will enable faster diagnosis and implementation of effective therapy, thereby minimizing the negative social impact.