bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fibrin Stiffness Regulates Phenotypic Plasticity of Metastatic Breast Cancer Cells
Authors: Heilala Maria , Lehtonen Arttu , Arasalo Ossi , Peura Aino , Pokki Juho , Ikkala Olli , Nonappa  , Klefström Juha , Munne Pauliina M. 
Published: September 2023

AbstractThe extracellular matrix (ECM)‐regulated phenotypic plasticity is crucial for metastatic progression of triple negative breast cancer (TNBC). While ECM faithful cell‐based models are available for in situ and invasive tumors, such as cell aggregate cultures in reconstituted basement membrane and in collagenous gels, there are no ECM faithful models for metastatic circulating tumor cells (CTCs). Such models are essential to represent the stage of metastasis where clinical relevance and therapeutic opportunities are significant. Here, CTC‐like DU4475 TNBC cells are cultured in mechanically tunable 3D fibrin hydrogels. This is motivated, as in circulation fibrin aids CTC survival by forming a protective coating reducing shear stress and immune cell‐mediated cytotoxicity and promotes several stages of late metastatic processes at the interface between circulation and tissue. This work shows that fibrin hydrogels support DU4475 cell growth, resulting in spheroid formation. Furthermore, increasing fibrin stiffness from 57 to 175 Pa leads to highly motile, actin and tubulin containing cellular protrusions, which are associated with specific cell morphology and gene expression patterns that markedly differ from basement membrane or suspension cultures. Thus, mechanically tunable fibrin gels reveal specific matrix‐based regulation of TNBC cell phenotype and offer scaffolds for CTC‐like cells with better mechano‐biological properties than liquid.
HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow Human iPSC‐Derived Cardiomyocytes Exhibit Low Immunogenicity for Heart Regeneration
Authors: Fang Yi‐Hsien , Wang Saprina P. H. , Liao I‐Chuang , Tsai Kuen‐Jer , Huang Po‐Hsien , Yang Pei‐Jung , Yen Chia‐Jui , Liu Ping‐Yen , Shan Yan‐Shen , Liu Yen‐Wen 
Published: September 2023

AbstractAlthough human pluripotent stem cells (hPSCs)‐derived cardiomyocytes (hPSC‐CMs) can remuscularize infarcted hearts and restore post‐infarct cardiac function, post‐transplant rejection resulting from human leukocyte antigen (HLA) mismatching is an enormous obstacle. It is crucial to identify hypoimmunogenic hPSCs for allogeneic cell therapy. This study is conducted to demonstrate the immune privilege of HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow human induced pluripotent stem cell (hiPSC)‐derived cardiomyocytes (hiPSC‐CMs). Ischemia‐reperfusion surgery is done to create transmural myocardial infarction in rats. At post‐infarct 4 days, hPSC‐CMs (1.0×107 cells per kg), including human embryonic stem cell‐derived cardiomyocytes (hESC‐CMs), HLA‐Elow/HLA‐Glow/HLA‐IIhigh hiPSC‐CMs, and HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow hiPSC‐CMs, are injected into the infarcted myocardium. Under the treatment of very low dose cyclosporine A (CsA), only HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow hiPSC‐CMs survive in vivo and improved post‐infarct cardiac function with infarct size reduction. HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow hiPSC‐CMs activate the SHP‐1 signaling pathway of natural killer (NK) cells and cytotoxic T cells to evade attack by NK cells and cytotoxic T cells. Herein, it is demonstrated that using a clinically relevant CsA dose, HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow hiPSC‐CMs repair the infarcted myocardium and restore the post‐infarct heart function. HLA‐Ehigh/HLA‐Ghigh/HLA‐IIlow hiPSCs are less immunogenic and may serve as platforms for regeneration medicine.
Realizing Unassisted Photo‐Charging of Zinc–Air Batteries by Anisotropic Charge Separation in Photoelectrodes
Authors: Kong Lingqiao , Ruan Qiushi , Qiao Jingyuan , Chen Pengyu , Yan Bingzhen , He Wei , Zhang Wei , Jiang Chaoran , Lu Chengjie , Sun ZhengMing 
Published: September 2023

AbstractSolar rechargeable zinc–air battery is a promising approach for capturing and storing intermittent solar energy through photoelectrochemical reactions. However, unassisted photo‐charging of zinc–air batteries is challenging due to suboptimal carrier accumulation on photoelectrodes, resulting in sluggish reaction kinetics. Here, unassisted photo‐charging of zinc–air battery is achieved by investigating anisotropic photogenerated charge separation on a series of representative semiconductors (ZnIn2S4, TiO2, and In2O3), among which the exceptional anisotropic charge separation on a ZnIn2S4 photoelectrode is revealed based on anisotropic charge diffusion capabilities. The charge separation is facet‐dependent, which is observed using Kelvin probe force microscopy, verifying a cause‐and‐effect relationship between the photo‐charge accumulation on photoelectrodes and their photo‐charging performance in zinc–air batteries. This work achieves an unassisted photo‐charging current density of 1.9 mA cm−2 with a light‐to‐chemical energy conversion efficiency of 1.45%, highlighting the importance of anisotropic semiconductors for unassisted photo‐charging of zinc–air batteries via efficient photogenerated charge separation.
High‐Efficiency Area‐Emissive White Organic Light‐Emitting Transistor for Full‐Color Display
Authors: Miao Zhagen , Gao Can , Gao Haikuo , Qin Zhengsheng , Hu Wenping , Dong Huanli 
Published: September 2023

AbstractThe construction of high‐performance white organic light‐emitting transistor (OLET) with uniform area emission is crucial for smart display technologies but remains greatly challenging. Herein, high‐efficiency uniform area‐emissive OLETs based on a unique lateral‐integrated device configuration which incorporates efficient energy transfer of phosphorescent and fluorescent guests, enabling color‐tunable and white emission, are demonstrated. Through precisely regulating the energy transfer between host and guests, high external quantum efficiency of 13.9% for white‐emission OLETs is achieved due to the improved high exciton utilization and light outcoupling efficiency which is the highest value reported so far for OLETs and prevents exciton‐charge annihilation and electrode photon losses. Moreover, good loop stability is also achieved, along with effective gate tunability and ultralow driving voltage of below 5 V. Finally, a 4 × 6 white‐emission OLET array for full‐color display is demonstrated for the first time, suggesting its great potential applications for advanced display technologies.
Unprecedented Aqueous Solubility of TEMPO and its Application as High Capacity Catholyte for Aqueous Organic Redox Flow Batteries
Authors: Pedraza Eduardo , de la Cruz Carlos , Mavrandonakis Andreas , Ventosa Edgar , Rubio‐Presa Rubén , Sanz Roberto , Senthilkumar Sirugaloor Thangavel , Navalpotro Paula , Marcilla Rebeca 
Published: September 2023

AbstractDespite the excellent electrochemical properties of non‐functionalized 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO), its use in aqueous organic redox flow battery (AORFB) is hindered to date due to its insolubility in water. However, in this study, an unprecedented solubility of 5.6 m is demonstrated in an aqueous solution of lithium bis(trifluoromethanesulfonyl)imide (LiTFSI), which is 80 times higher than in water (0.07 m). A computational study reveals that the unique interaction between TEMPO and TFSI is essential to achieve this record solubility. TEMPO catholytes are tested in symmetric flow cells, demonstrating high capacity (23.85 Ah L−1), high material utilization (89%), and robust reversible performance with long‐term stability (low capacity fading of 0.082%/day). When paired with sulfonated viologen anolyte ((SPr2)V), an AORFB with low capacity fading over cycling (0.60%/day, 0.048%/cycle) is achieved, constituting the first example of a non‐functionalized TEMPO catholyte for AORFB. Notably, this solubilization strategy could be applied to other unexplored chemistries in aqueous electrolytes, leading to the development of new AORFBs.
Phylogenomic and morphological data reveal hidden patterns of diversity in the national tree of Brazil, Paubrasilia echinata
Authors: Rees Mathew , Neaves Linda E. , Lewis Gwilym P. , de Lima Haroldo C. , Gagnon Edeline 
Published: September 2023

AbstractPremisePaubrasilia echinata (common names, pau brasil, brazilwood) is the national tree of Brazil and an endangered species endemic to the Brazilian Atlantic Forest. Over its wide distribution of 2000 km, its leaflets morphology exhibits extensive plasticity. Three morphotypes are commonly identified based on leaf size, but it is unclear if they represent distinct taxa or a single polymorphic species. This study aims to clarify the taxonomic position of the three morphotypes to inform conservation decisions.MethodsA morphometric study of leaf characters of herbarium specimens was coupled with genetic analyses using genotype‐by‐sequencing data. We used maximum‐likelihood and coalescent methods to evaluate the phylogenetic and population structure of the species. We compared these with a morphological dendrogram built from hierarchical clustering.ResultsTwo of the three morphotypes formed separately evolving lineages, the third morphotype formed two geographically separate lineages, and northern trees with intermediate leaf morphology formed a separate fifth lineage. Leaflet size varied by over 35‐fold, and although morphological clustering generally matched the genetic patterns, there were some overlaps, highlighting the cryptic diversity within this group.ConclusionsOur genetic and morphological results provide some evidence that cultivated trees from different states in Brazil seem to have a limited genetic origin and do not reflect the broader genetic and geographical diversity of the species. As a result, more care is likely needed to preserve the overall genomic diversity of this endangered and iconic species.
Uncertainty and disturbance estimator‐based geometry tracking control for quadrotors without linear velocity measurements
Authors: Hu Yang , Miao Zhiqiang , Wang Yaonan , Lin Jie , Lin Qiong 
Published: September 2023

AbstractThis paper addresses the trajectory tracking control problem of a quadrotor under uncertain model parameters, external disturbances, and unmeasurable linear velocity. The main contribution of this paper is to present a geometric control strategy with a simple structure and easily adjustable parameters, which combines the differentiator and uncertainty and disturbance estimator techniques to achieve global trajectory tracking control. First, a cascade control framework is introduced to solve the strong coupling and underactuated problems of quadrotors. Furthermore, an antidisturbance outer‐loop differentiator‐based position controller is designed based on the uncertainty and disturbance estimators (UDE) to achieve accurate quadrotor tracking of the desired trajectory. The higher order control inputs of the translational dynamics are estimated using a differentiator to determine the desired angular velocity and its derivative. Then, a robust inner‐loop geometric controller is derived in Lie algebraic space using the rotation matrix. After that, the stability analysis of the closed‐loop system is executed using the Lyapunov stability theory. Finally, the numerical simulation verifies that the method enables the quadrotor to have strong robustness and excellent tracking capability in the presence of large initial angles, time‐varying disturbances, and unmeasured linear velocity.
What drives consumer expectations for institutional food? Identifying antecedents of normative and predictive expectations in baby boomers
Authors: Andreassen Hanne , Gjerald Olga 
Published: September 2023

AbstractConsumer expectations play a crucial role in shaping consumer experiences, and it is important to understand what factors contribute to these expectations. However, there is limited research on the factors that influence expectations, particularly internal factors. Additionally, few studies differentiate between types of expectations and their underlying causes. This study focuses on normative and predictive expectations related to institutional food and examines how individual psychological factors (such as entitlement, subjective knowledge, and sensitivity to disconfirmation) and sociocultural factors (like word of mouth and temporal focus) influence these expectations. The researchers used surveys to collect data from two different groups of baby boomers in Norway, with a total of 300 participants in each group. The findings reveal that normative and predictive expectations for institutional food differ in their content, and individual psychological and sociocultural factors affect different types of expectations. Individual psychological factors have a stronger influence on normative expectations, whereas sociocultural factors primarily impact predictive expectations. Further research should explore the relationship between the content of expectations and their drivers in other contexts. This study contributes to our understanding of the factors that shape consumer expectations, particularly in non‐hedonic consumption experiences.
[Fe]‐Hydrogenase, Cofactor Biosynthesis and Engineering
Authors: Arriaza‐Gallardo Francisco J. , Zheng Yu‐Cong , Gehl Manuel , Nomura Shunsuke , Fernandes‐Queiroz J. Pedro , Shima Seigo 
Published: September 2023

Abstract[Fe]‐hydrogenase catalyzes the heterolytic cleavage of H2 and reversible hydride transfer to methenyl‐tetrahydromethanopterin. The iron‐guanylylpyridinol (FeGP) cofactor is the prosthetic group of this enzyme, in which mononuclear Fe(II) is ligated with a pyridinol and two CO ligands. The pyridinol ligand fixes the iron by an acyl carbon and a pyridinol nitrogen. Biosynthetic proteins for this cofactor are encoded in the hmd co‐occurring (hcg) genes. The function of HcgB, HcgC, HcgD, HcgE, and HcgF was studied by using structure‐to‐function analysis, which is based on the crystal structure of the proteins and subsequent enzyme assays. Recently, we reported the catalytic properties of HcgA and HcgG, novel radical S‐adenosyl methionine enzymes, by using an in vitro biosynthesis assay. Here, we review the properties of [Fe]‐hydrogenase and the FeGP cofactor, and the biosynthesis of the FeGP cofactor. Finally, we discuss the expected engineering of [Fe]‐hydrogenase and the FeGP cofactor.
4‐Oxo‐β‐Lactams as Novel Inhibitors for Rhomboid Proteases
Authors: Yang Jian , Carvalho Luís A. R. , Ji Shanping , Chen Suyuan , Moreira Rui , Verhelst Steven H. L. 
Published: September 2023

AbstractIntramembrane serine proteases (rhomboid proteases) are involved in a variety of biological processes and are implicated in several diseases. Here, we report 4‐oxo‐β‐lactams as a novel scaffold for inhibition of rhomboids. We show that they covalently react with the active site and that the covalent bond is sufficiently stable for detection of the covalent rhomboid‐lactam complex. 4‐Oxo‐β‐lactams may therefore find future use as both inhibitors and activity‐based probes for rhomboid proteases.
CO2 Hydrogenation to CH3OH over Cu‐Based Catalysts: Primary and Side Reactions
Authors: Zhao Dan , Han Shanlei , Kondratenko Evgenii V. 
Published: September 2023

AbstractCarbon dioxide (CO2) hydrogenation to methanol (CH3OH) is one of the most promising approaches to provide this platform chemical and to close carbon cycles. In this minireview, we systematically analyze primary and secondary reactions which can take place in this reaction over Cu‐based catalysts. In addition to repeatedly discussed reverse water gas shift reaction (RWGS) and CH3OH production directly from CO2, we consider decomposition, dehydration, dehydrogenation, and steam reforming of the desired alcohol. These reactions are usually ignored in the studies dealing with CO2 hydrogenation to CH3OH but can worsen the catalyst efficiency. Apart from the corresponding thermodynamic analysis, proposed reaction mechanisms and active sites are described and discussed. The effects of co‐fed water, CH3OH and methyl formate on catalyst performance are critically scrutinized, too. We also provide several criteria for unambiguous comparison of different catalysts in terms of CH3OH selectivity and their activity.
Synthesis of Chrysanthemum‐Like BiOBr Microspheres by Structure Induction of Chitosan for Enhancing Photocatalytic Activity
Authors: Geng Yuanze , Zhang Zhancheng , Zhang Yaping , Cao Peiyu , He Yufeng , Wang Rongmin 
Published: September 2023

AbstractPhotocatalysis is a green technology with important application prospects in environmental fields such as wastewater treatment. Herein, we synthesized chrysanthemum‐like BiOBr microspheres (CLB0.45) by introduction of structure guide and soft‐template. Natural biomass chitosan (CS) in the precursor of BiOBr successfully suppressed the growth of both (001) and (102) lattice planes through the solvent effect, and promoted the exposure of (110) lattice planes that dominated the photocatalytic performance. Significantly, the polymer chains regulated the nanosheets of BiOBr to accurately self‐assemble. The obtained CLB0.45 had narrower band gap and more active sites due to surface defects, and the photocatalysts have higher photo‐electron hole pairs separation efficiency. Additionally, the degradation performance towards rhodamine B (RhB; 99.7 %, 30 min) of the CLB0.45 was increased, which was 4.6 times higher than that of pure BiOBr. The degradation rate of tetracycline (TC) was also excellent (85.3 %). The photodegradation mechanism of CLB0.45 was proposed and verified. In summary, the prepared CLB0.45, obtained through structure guiding and using a soft template, have a promising future in the photocatalytic purification of organic pollutants.
End‐to‐End Semi‐automated Mid‐scale Protein Screening Platform for Drug Discovery Research
Authors: Zilberleyb Inna , Kugel Christine , Patel Purvit , Tam Christine , Hsu Peter L. , Franke Yvonne , Pahuja Kanika Bajaj 
Published: September 2023

AbstractThe drug discovery landscape is ever‐evolving and constantly demands revolutionary technology advancements in protein expression and production laboratories. We have built a higher‐throughput mid‐scale semi‐automated protein expression and screening platform to accelerate drug discovery research. The workflow described here enables comprehensive expression and purification screening assessment of challenging or difficult‐to‐express recombinant proteins in a fast and efficient manner by delivering small but sufficient amounts of high‐quality proteins. The platform has been implemented for a wide range of applications that include identification of optimal constructs and chaperones for poorly expressing proteins, assessment of co‐expression partners for expressing stable multiprotein complexes, and suitable buffer/additive screening for insoluble or aggregation‐prone proteins. The approach allows parallel expression, purification, and characterization of 24 different samples using co‐infection or a polycistronic approach in insect cells and enables parallel testing of multiple parameters to improve protein yields. The strategy has been successfully adopted for screening intracellular and secreted proteins in Escherichia coli, mammalian transient expression, and baculovirus expression vector systems. Proteins purified from this platform are used for several structural and functional screens, such as negative staining, biochemical activity assays, mass spectrometry, surface plasmon resonance, and DNA‐encoded chemical library screens. In this article, for simplicity, we have focused on detailed expression and purification screening of intracellular protein complexes from insect cells. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Baculovirus generation via homologous recombinationSupport Protocol 1: Anti‐glycoprotein 64 antibody assayBasic Protocol 2: Generation of insect cell biomass expressing target protein(s)Basic Protocol 3: Mid‐scale affinity purificationSupport Protocol 2: Automated method for affinity purification on Hamilton STARBasic Protocol 4: Size exclusion chromatographySupport Protocol 3: Chromeleon 7 operation on Vanquish Duo
Historically restricted or historically empowered? Differences in access to historical content knowledge between low‐ and high‐SES pupils
Authors: Rosenlund David , Persson Magnus 
Published: September 2023

AbstractIn the study presented in this article, the aim is to further the understanding regarding the differences between pupils (aged 15–16) from schools with low or high socio‐economic status (SES), regarding the amount and diversity of content knowledge in history that they have acquired by the end of compulsory schooling. Following a definition of historical content knowledge, we situate the concept in relation to other aspects of the history school subject. This is done to visualize historical content knowledge's central role in more complex aspects of the subject. The empirical material used in the study is pupils' responses on both selected and constructed response items on the Swedish national test in history. In the study, a combination of qualitative and quantitative approaches is used. The results show not only that pupils in low‐SES schools provide fewer examples of historical content knowledge. We can also establish that the historical content knowledge of pupils from high‐SES schools represents several perspectives while there are few perspectives present in the responses from pupils in low‐SES schools. The results are used to discuss how the differences between pupils in low‐ and high‐SES schools may affect their possibilities for educational success and active participation in society.
Cyberbullying and eating disorder symptoms in US early adolescents
Authors: Cheng Chloe M. , Chu Jonathan , Ganson Kyle T. , Trompeter Nora , Testa Alexander , Jackson Dylan B. , He Jinbo , Glidden David V. , Baker Fiona C. , Nagata Jason M. 
Published: September 2023

AbstractObjectiveThe objective of this study was to determine the association between cyberbullying and eating disorder symptoms in a national sample of 10–14‐year‐old early adolescents.MethodWe analyzed cross‐sectional data from the Adolescent Brain Cognitive Development (ABCD) Study (Year 2, 2018–2020, N = 10,258/11,875, 49% female, 46% non‐White). Data were collected using multi‐stage probability sampling. Modified Poisson regression analyses examined the association between cyberbullying and self‐reported eating disorder symptoms based on the Kiddie Schedule for Affective Disorders and Schizophrenia (KSADS‐5).ResultsCyberbullying victimization was associated with worry about weight gain (prevalence ratio [PR] 2.41, 95% confidence interval [CI] 1.48–3.91), self‐worth tied to weight (PR 2.08, 95% CI 1.33–3.26), inappropriate compensatory behavior to prevent weight gain (PR 1.95, 95% CI 1.57–2.42), binge eating (PR 1.95, 95% CI 1.59–2.39), and distress with binge eating (PR 2.64, 95% CI 1.94–3.59), in models adjusting for potential confounders. Cyberbullying perpetration was associated with worry about weight gain (PR 3.52, 95% CI 1.19–10.37), self‐worth tied to weight (PR 5.59, 95% CI 2.56–12.20), binge eating (PR 2.36, 95% CI 1.44–3.87), and distress with binge eating (PR 2.84, 95% CI 1.47–5.49).DiscussionCyberbullying victimization and perpetration in early adolescence are associated with eating disorder symptoms. Clinicians may consider assessing for cyberbullying and eating disorder symptoms in early adolescence and provide anticipatory guidance.Public Significance StatementEating disorders often onset in adolescence and have among the highest mortality rates of any psychiatric disorder. In addition, cyberbullying has increased in prevalence among adolescents and significantly impacts mental health. In a national study of early adolescents, we found that cyberbullying victimization and perpetration are associated with eating disorder symptoms. Screening for and providing anticipatory guidance on cyberbullying and eating disorder symptoms in early adolescents may be warranted.