bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification, molecular characterization and phylogenetic analysis of a novel nucleorhabdovirus infecting Paris polyphylla var. yunnanensis
Authors: Hu Jingyu , Miao Tianli , Que Kaijuan , Rahman Md. Siddiqur , Zhang Lei , Dong Xian , Ji Pengzhang , Dong Jiahong 
Published: June 2023

AbstractA novel betanucleorhabdovirus infecting Paris polyphylla var. yunnanensis, tentatively named Paris yunnanensis rhabdovirus 1 (PyRV1), was recently identified in Yunnan Province, China. The infected plants showed vein clearing and leaf crinkle at early stage of infection, followed by leaf yellowing and necrosis. Enveloped bacilliform particles were observed using electron microscopy. The virus was mechanically transmissible to Nicotiana bethamiana and N. glutinosa. The complete genome of PyRV1 consists of 13,509 nucleotides, the organization of which was typical of rhabdoviruses, containing six open reading frames encoding proteins N–P–P3–M–G–L on the anti-sense strand, separated by conserved intergenic regions and flanked by complementary 3′-leader and 5′-trailer sequences. The genome of PyRV1 shared highest nucleotide sequence identity (55.1%) with Sonchus yellow net virus (SYNV), and the N, P, P3, M, G, and L proteins showed 56.9%, 37.2%, 38.4%, 41.8%, 56.7%, and 49.4% amino acid sequence identities with respective proteins of SYNV, suggesting RyRV1 belongs to a new species of the genus Betanucleorhabdovirus.
Effect of laser shock peening on microstructure and mechanical properties of laser cladding 30CrMnSiNi2A high-strength steel
Authors: Wang Lingfeng , Yu Kun , Cheng Xing , Cao Tong , Zhou Liucheng 
Published: June 2023

AbstractThe effect of laser shock peening (LSP) on the microhardness and tensile properties of laser cladding (LC) 30CrMnSiNi2A high-strength steel was studied. After LSP, the microhardness of the cladding zone reached approximately 800 HV0.2, which was 25% higher than that of the substrate, while the cladding zone without LSP had an approximately 18% increase in its microhardness. Two strengthening processes were designed: groove LSP + LC + surface LSP versus LC + surface LSP. The former's tensile strength and yield strength were less than 10% weaker than those of forged materials, which is the best mechanical property recovery found in LC samples. The microstructural characteristics of the LC samples were analysed by scanning electron microscopy (SEM) and electron backscatter diffraction. Under the action of the laser-induced shock wave, the grain size of the LC sample surface was refined, the low-angle grain boundaries on the surface layer increased significantly, and the austenite grain length was reduced from 30–40 μm in the deep layer to 4–8 μm in the surface layer. In addition, LSP modulated the residual stress field, hence preventing the weakening effect of the LC process's thermal stress on the components' mechanical properties.
A validated prognostic nomogram for patients with H3 K27M-mutant diffuse midline glioma
Authors: Peng Youheng , Ren Yanming , Huang Bowen , Tang Jun , Jv Yan , Mao Qing , Liu Yanhui , Lei Yinjie , Zhang Yuekang 
Published: June 2023

AbstractH3 K27M-mutant diffuse midline glioma (H3 K27M-mt DMG) is a rare, highly invasive tumor with a poor prognosis. The prognostic factors of H3 K27M-mt DMG have not been fully identified, and there is no clinical prediction model for it. This study aimed to develop and validate a prognostic model for predicting the probability of survival in patients with H3 K27M-mt DMG. Patients diagnosed with H3 K27M-mt DMG in the West China Hospital from January 2016 to August 2021 were included. Cox proportional hazard regression was used for survival assessment, with adjustment for known prognostic factors. The final model was established using the patient data of our center as the training cohort and data from other centers for external independent verification. One hundred and five patients were ultimately included in the training cohort, and 43 cases from another institution were used as the validation cohort. The factors influencing survival probability in the prediction model included age, preoperative KPS score, radiotherapy and Ki-67 expression level. The adjusted consistency indices of the Cox regression model in internal bootstrap validation at 6, 12, and 18 months were 0.776, 0.766, and 0.764, respectively. The calibration chart showed high consistency between the predicted and observed results. The discrimination in external verification was 0.785, and the calibration curve showed good calibration ability. We identified the risk factors that affect the prognosis of H3 K27M-mt DMG patients and then established and validated a diagnostic model for predicting the survival probability of these patients.
Controlling grain structure in metallic additive manufacturing using a versatile, inexpensive process control system
Authors: Chechik Lova , Goodall Alexander D. , Christofidou Katerina A. , Todd Iain 
Published: June 2023

AbstractAdditive manufacturing (AM), commonly termed 3D printing, is a revolutionary manufacturing technology with great industrial relevance in the aerospace, medical and automotive sectors. Metallic AM allows creation of complex intricate parts and repair of large components; however, certification is currently a concern due to lack of process consistency. A versatile, inexpensive process control system was developed and integrated, reducing variability in melt pool fluctuation and improving microstructural homogeneity of components. Remnant microstructural variation can be explained by the change in heat flow mechanism with geometry. The grain area variability was reduced by up to 94% at a fraction of the cost of a typical thermal camera, with control software written in-house and made publically available. This decreases the barrier to implementation for process feedback control, which can be implemented in many manufacturing processes, from polymer AM to injection moulding to inert-gas heat treatment.
The characterization of collagen-based scaffolds modified with phenolic acids for tissue engineering application
Authors: Kaczmarek-Szczepańska Beata , Polkowska Izabela , Małek Marcin , Kluczyński Janusz , Paździor-Czapula Katarzyna , Wekwejt Marcin , Michno Anna , Ronowska Anna , Pałubicka Anna , Nowicka Beata , Otrocka-Domagała Iwona 
Published: June 2023

AbstractThe aim of the experiment was to study the morphology of collagen-based scaffolds modified by caffeic acid, ferulic acid, and gallic acid, their swelling, and degradation rate, as well as the biological properties of scaffolds, such as antioxidant activity, hemo- and cytocompatibility, histological observation, and antibacterial properties. Scaffolds based on collagen with phenolic acid showed higher swelling rate and enzymatic stability compared to scaffolds based on pure collagen, and the radical scavenging activity was in the range 85–91%. All scaffolds were non-hemolytic and compatible with surrounding tissues. Collagen modified by ferulic acid showed potentially negative effects on hFOB cells as a significantly increased LDH release was found, but all of the studied materials had antimicrobial activity against Staphylococcus aureus and Escherichia coli. It may be assumed that phenolic acids, such as caffeic, ferulic, and gallic acid, are modifiers and provide novel biological properties of collagen-based scaffolds. This paper provides the summarization and comparison of the biological properties of scaffolds based on collagen modified with three different phenolic acids.
Partition of Omega-like facility into two configurations of 24 and 36 laser beams to improve implosion performance
Authors: Temporal M. , Piriz A. R. , Canaud B. , Ramis R. , Craxton R. S. 
Published: June 2023

AbstractAn Omega-like beam configuration is considered where the 60-beam layout can be separated into two independent sub-configurations with 24 and 36 laser beams, each minimizing direct drive illumination non-uniformity. Two different laser focal spot profiles, one associated with each configuration, are proposed to apply the zooming technique in order to increase the laser-target coupling efficiency. This approach is used by 1D hydrodynamics simulations of the implosion of a direct-drive capsule characterized by a relatively large aspect ratio A = 7 and an optimized laser pulse shape delivering a maximum of 30 TW and 30 kJ, with different temporal pulse shapes in each of the two sets of beams. It is shown that zooming allows for an optimistic 1D thermonuclear energy gain greater than one while without zooming the thermonuclear gain remains largely below one. While this is incompatible with the as-built Omega laser, it provides a promising option for a future intermediate-energy direct drive laser system.
Additive fabrication and experimental validation of a lightweight thermoelectric generator
Authors: Fanciulli Carlo , Abedi Hossein , Nespoli Adelaide , Dondè Roberto , La Terra Caterina , Migliorini Francesca , Passaretti Francesca , De Iuliis Silvana 
Published: June 2023

AbstractWe develop a thermoelectric generator based on catalytic combustion and operating in the low power range (up to 10 W). Considering the target of small-scale thermoelectric generators, the additive technique was chosen as an enabling technology to customize the different parts of the presented device. The generator consists of a hexagonal shaped combustion chamber coupled to commercial thermoelectric modules, water-cooled at the cold side. Thanks to the components design, heat transfer across each part of the system is properly driven enhancing the thermal management of the system. Moreover, in order to improve the overall efficiency, exhausts outlet is designed to promote heat recovery. The generator is characterized achieving an electrical power output close to 9 W in continuous regime, with an overall efficiency of 3.55%. The compact size, the light weight, the simple design and the reliability in continuous operating conditions are all promising features of the device described. Furthermore, the materials chosen for the device can suggest a way to fabricate cheaper heat exchangers, actually one of the main costs of the device development.
Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish
Authors: Nanjappa Dechamma Pandyanda , De Saffel Hanna , Kalladka Krithika , Arjuna Srividya , Babu Nishith , Prasad Kishan , Sips Patrick , Chakraborty Anirban 
Published: June 2023

AbstractPoly (A)-specific ribonuclease (PARN) is the most important 3′–5′exonuclease involved in the process of deadenylation, the removal of poly (A) tails of mRNAs. Although PARN is primarily known for its role in mRNA stability, recent studies suggest several other functions of PARN including a role in telomere biology, non-coding RNA maturation, trimming of miRNAs, ribosome biogenesis and TP53 function. Moreover, PARN expression is de-regulated in many cancers, including solid tumours and hematopoietic malignancies. To better understand the in vivo role of PARN, we used a zebrafish model to study the physiological consequences of Parn loss-of-function. Exon 19 of the gene, which partially codes for the RNA binding domain of the protein, was targeted for CRISPR-Cas9-directed genome editing. Contrary to the expectations, no developmental defects were observed in the zebrafish with a parn nonsense mutation. Intriguingly, the parn null mutants were viable and fertile, but turned out to only develop into males. Histological analysis of the gonads in the mutants and their wild type siblings revealed a defective maturation of gonadal cells in the parn null mutants. The results of this study highlight yet another emerging function of Parn, i.e., its role in oogenesis.
MoBiDICT: New 3D static models of close synchronised binaries in hydrostatic equilibrium
Authors: Fellay L. , Dupret M.-A. 
Published: June 2023

Context. In close binary systems, tidal interactions and rotational effects can strongly influence stellar evolution as a result of mass transfer and common envelope phases. These aspects can only be treated following improvements of theoretical models to take into account the breaking of spherical symmetry occurring in close binaries. Current models of binary stars rely on either the so-called Roche model or the perturbative approach, both of which result in several assumptions concerning the gravitational, tidal, and centrifugal potentials.
Aims. Our aim is to develop a precise 3D model of stellar deformations and to study the robustness of the Roche and perturbative models in different deformation regimes.
Methods. We developed a new non-perturbative method to compute the precise structural deformation of a binary system in three dimensions that is valid even in the most distorted cases. We then compared our new method to the Roche and perturbative models for different orbital separations and binary components.
Results. We found that in the most distorted cases, both the Roche and perturbative models significantly underestimate the deformation of binaries. The effective gravity and the overall structural deformations are also noticeably different in the most distorted cases, leading to modifications of the usual gravity darkening generally obtained through the Roche model when interpreting the observations. Moreover, we found that the dipolar term of the gravitational potential, usually neglected by the perturbative theory, has the same order of magnitude as the leading tidal term in the most distorted cases.
Conclusions. We developed a new method that is capable of precisely computing the deformations of a binary system composed of any type of stars, even compact objects. For all the stars we studied, the differences in deformation with respect to the Roche or perturbative models are significant in the most distorted cases, which impacts both the interpretation of observations and the theoretical structural depiction of these distorted bodies. In the weaker deformation regimes, the Roche model is a viable option for studying the surface properties of binaries, while the perturbative model is strongly favoured when evaluating structural deformations.
Thrombembolie-Risikofaktoren und Prophylaxe
Authors: Paffenholz Pia 
Published: June 2023

Zusammenfassung
Venöse Thromboembolien stellen eine wichtige Komplikation bei Tumorpatienten, da sie bei diesen Patienten gehäuft auftreten und eine relevante Morbidität bedingen. Das Risiko für thromboembolische Komplikationen bei Tumorpatienten liegt 3–9-mal höher als bei Nichttumorpatienten und stellt die zweithäufigste Todesursache bei Tumorpatienten dar. Das Thromboserisiko hängt neben der tumorinduzierten Koagulopathie von individuellen Faktoren, der Art und dem Stadium der Krebserkrankung, der Zeit seit der Krebsdiagnose sowie der Art einer systemischen Krebstherapie ab. Eine Thromboseprophylaxe bei Tumorpatienten ist wirksam, kann jedoch mit erhöhten Blutungen einhergehen. Auch wenn dezidierte Empfehlungen für einzelne Tumorentitäten aktuell nicht vorliegen, empfehlen internationale Leitlinien prophylaktische Maßnahmen bei Hochrisikopatienten. So kann ein Thromboserisiko von >8–10% als Indikation für eine Thromboseprophylaxe angesehen werden. Dieses Risiko wurde bei Patienten mit einem Khorana-Score von ≥2 erreicht und sollte individuell mithilfe der Nomogramme berechnet werden. Vor allem bei Patienten mit geringem Blutungsrisiko wird diese Empfehlung ausgesprochen. Während des Arztgesprächs sollten zudem Risikofaktoren und Symptome eines thromboembolischen Events thematisiert und Materialien zur Patientenaufklärung ausgehändigt werden.
Drug Reprofiling: A Prospective Approach to Battle Chronic Ailments
Authors: Aggarwal Natasha Naval , Sindhoor S.M. , Naveen N. Raghavendra , Gowthami Buduru , Biju Prajitha 
Published: June 2023

AbstractThe concept of drug “reprofiling” has garnered attention in the recent past post the outbreak of coronavirus disease 2019 when traditional drug discovery seemed to fail. Even though repurposing is called pharma-friendly in terms of monetary relief, clinical trials play an integral role in repurposed nontarget /combination moieties. Nevertheless, when a drug exhibits no returns to the market, an exhaustive study on mechanism of action (MOA) can help for reprofiling of drugs for new indications. However, several papers have claimed that scarcity of resources and data access, and staffing issues, tends to pull down reprofiling of drugs. In contrast to this notion, a total of 155 patented articles to date give a strong base for drug repurposing. In the present review, a scientific prospection of reprofiled antifungal and antiviral agents for the past decade was made using the PubMed database wherein a total of 410 and 768 publications have resulted respectively. The authors have attempted to focus their attention to repurposing antifungal drugs for chronic ailments and infectious diseases by understanding their MOA.For example, antifungal azoles, which work by blocking ergosterol synthesis, can be repurposed as they inhibit histone deacetylase as well significantly decrease the production of cytokines and modulate the inflammatory pathways used by cancer cells.Hence, we believe that the mentioned Food and Drug Administration-approved drug candidates can be utilized to treat nontarget diseases, notably rare/neglected diseases as well as chronic illnesses and the more recent viral infections that are spreading globally.
Mental Health Disorders in Circumcised Reproductive-age Women, Legal Dimensions and Prevention Strategies: A Narrative Review
Authors: Alidost Farzane , Abbasi Mahmoud , Ghamsari Sepideh Rezaei , Pakzad Mona 
Published: June 2023

Abstract
Objective: Female genital mutilation/cutting (FGM/C) can affect women's lives through various physical, psychological, social and even sexual mechanisms. According to the World Health Organization guidelines for managing the health effects of FGM/C, further research into its psychological effects and preventative measures is required. In this study, a comprehensive review of the mental health consequences of circumcised women of reproductive age has been conducted with a special focus on providing preventive solutions.
Methods: A comprehensive search of the Web of Science, PubMed(MEDLINE), Proquest, Scopus and Google scholar was carried outfrom 2000 to 2022. The second stage of search was conducted in grey literature. To facilitate a systematic approach to search the literature, the PECO framework, was adopted.
Results: The result of this narrative review study showed that, the most common mental health disorder in reproductive age circumcised women were depression, anxiety and post-traumatic stress disorder. Some studies found a significant relationship between parents' education level and circumcised girls, so that parents of the circumcised women had a low level of education. Two studies considered religious beliefs, tradition, cleanness, sexual desire control and virginity as the reasons for FGM/C.
Conclusion: All forms of FGM/C may be harmful to one's health. Women, who have undergone widespread forms of circumcision, are more likely to develop mental disorders. As the psychosocial effects of circumcision can affect the sexual experience of circumcised women, addressing this issue, emphasizing its legal aspects, and providing preventative solutions can improve physical, mental, social, and even sexual health in circumcised women.
Spin current generation from an epitaxial tungsten dioxide WO2
Authors: Ueda Kohei , Fujii Hayato , Kida Takanori , Hagiwara Masayuki , Matsuno Jobu 
Published: June 2023

We report on efficient spin current generation at room temperature in rutile-type WO2 grown on an Al2O3(0001) substrate. The optimal WO2 film has a (010)-oriented monoclinically distorted rutile structure with metallic conductivity due to 5d2 electrons, as characterized by x-ray diffraction, electronic transport, and x-ray photoelectron spectroscopy. By conducting harmonic Hall measurement in a Ni81Fe19/WO2 bilayer, we estimate two symmetries of the spin–orbit torque (SOT), i.e., dampinglike (DL) and fieldlike ones, to find that the former is larger than the latter. By comparison with the Ni81Fe19/W control sample, the observed DL SOT efficiency ξDL of WO2 (+0.174) is about two-thirds of that of W (−0.281) in magnitude, with a striking difference in their signs. The magnitude of the ξDL of WO2 exhibits a comparable value to those of widely reported Pt and Ta, and Ir oxide IrO2. The positive sign of the ξDL of WO2 can be explained by the preceding theoretical study based on the 4d oxides. These results highlight that the epitaxial WO2 offers a great opportunity of rutile oxides with spintronic functionalities, leading to future spin–orbit torque-controlled devices.
Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryoelectron microscopy
Authors: Watkins Maxwell B. , Wang Haoyue , Burnim Audrey , Ando Nozomi 
Published: June 2023

Cobalamin-dependent methionine synthase (MetH) catalyzes the synthesis of methionine from homocysteine and 5-methyltetrahydrofolate (CH
3
-H
4
folate) using the unique chemistry of its cofactor. In doing so, MetH links the cycling of
S
-adenosylmethionine with the folate cycle in one-carbon metabolism. Extensive biochemical and structural studies on
Escherichia coli
MetH have shown that this flexible, multidomain enzyme adopts two major conformations to prevent a futile cycle of methionine production and consumption. However, as MetH is highly dynamic as well as both a photosensitive and oxygen-sensitive metalloenzyme, it poses special challenges for structural studies, and existing structures have necessarily come from a “divide and conquer” approach. In this study, we investigate
E. coli
MetH and a thermophilic homolog from
Thermus filiformis
using small-angle X-ray scattering (SAXS), single-particle cryoelectron microscopy (cryo-EM), and extensive analysis of the AlphaFold2 database to present a structural description of the full-length MetH in its entirety. Using SAXS, we describe a common resting-state conformation shared by both active and inactive oxidation states of MetH and the roles of CH
3
-H
4
folate and flavodoxin in initiating turnover and reactivation. By combining SAXS with a 3.6-Å cryo-EM structure of the
T. filiformis
MetH, we show that the resting-state conformation consists of a stable arrangement of the catalytic domains that is linked to a highly mobile reactivation domain. Finally, by combining AlphaFold2-guided sequence analysis and our experimental findings, we propose a general model for functional switching in MetH.
Indexing turn-beginnings in Norwegian Sign Language conversation
Authors: Ferrara Lindsay 
Published: June 2023

Abstract
It is well known that signers and speakers routinely produce finger points during interaction. While the
referential functions of such finger points have received great attention from researchers, they are also used to manage
interactions between interlocutors. These functions are less understood and have received less research focus. The current study
helps to redress this gap in the literature by investigating how finger pointing is used to index and coordinate turn-beginnings
in a corpus of 11 semi-naturalistic (Norwegian) signed language conversations, involving between two to five signers (3.4 hours of
signing). The data was initially annotated in ELAN and then further qualitative analysis was conducted. This investigation
revealed that finger pointing effectively indexes previous and upcoming discourse, thereby binding sequences of conversational
moves and guiding their trajectory, helping signers to coordinate turn transitions and interaction as it unfolds.