bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurological manifestations of an emerging zoonosis—Human monkeypox virus: A systematic review
Authors: Khan Sajjad Ahmed , Parajuli Surya Bahadur , Rauniyar Vivek K. 
Published: September 2023

Background:
The last few decades have witnessed an appalling rise in several emerging and re-emerging viral and zoonotic outbreaks. Amongst those emerging zoonosis, one of the diseases which is gaining popularity these days and has been declared as public health emergency of international concern by the world health organization, is human monkeypox virus (HMPX). Proper understanding of the clinical spectrum of the disease is of paramount importance for early diagnosis and treatment. In this review, we aimed to study and quantify the neurological manifestations of HMPX virus infection.

Methods:
Any study, released prior to April 13, 2023, that reported neurological manifestations in patients infected by HMPX virus were reviewed systematically on PubMed, Scopus, Google Scholar, and Cochrane Library using the PRISMA (Preferred Reporting Items for Systematic review and Meta-Analysis) statement.

Results:
Our systematic review included data from 22 eligible studies: 10 cohort studies, 3 cross sectional studies, one retrospective study, 5 case series, and 2 case reports. The most commonly reported neurological manifestations of HMPX were headache (48.84%), myalgia (27.50%), fatigue (17.73%), and photophobia (4.43%). Uncommonly, HMPX can also present with visual deficit (0.57%), seizure (0.34%), encephalitis (0.8%), dizziness (0.34%), encephalomyelitis (0.23%), coma (0.11%), and transverse myelitis (0.11%).

Discussions:
Monkeypox virus usually presents with self-limiting painful rash, lymphadenitis, and fever, complications like secondary skin infection, eye problems and pneumonia can be life threatening, carrying a case fatality rate of 1% to 10%. Neurological manifestations are not uncommon and can further add-on to morbidity and mortality.
LigaSure technique for splenectomy: A systematic review and meta-analysis
Authors: Huang Long , Yu Qingsheng , Peng Hui , Zhen Zhou 
Published: September 2023

Background:
This study aimed to clarify the optimal management of the LigaSure technique and conventional techniques during splenectomy.

Methods:
All databases, including CBM, CNKI, WFPD, Medline, EMBASE, PubMed, and Cochrane databases up to April 2023, were searched for relevant studies comparing the LigaSure technique with conventional techniques. Six studies, extracted by 2 independent reviewers, were evaluated for blood loss, operative time, conversion, mortality, hospital stay, and transfusion.

Results:
The blood loss was significantly higher in the convention group than in the LigaSure group (WMD = −48.98, 95% CI: −62.41 to −35.55, P < .00001). Meanwhile, the mean operative time was significantly shorter in LigaSure group than in convention group (WMD = −10.57; 95% CI: −12.35 to −8.78), P < .00001). No significant differences were found regarding the conversion rate, hospital stay, morbidity, and transfusion.

Conclusions:
The LigaSure technique has comparable effects to conventional techniques, but to some extent reduces blood loss and operative time.
Unveiling the “Kebab” technique: A case report on a two-stage reconstruction method for repeated complex cranioplasty
Authors: Chiang Liang-Jui , Lee Jing-Wei , Lee Po-Hsuan , Lee Jung-Shun 
Published: September 2023

Rationale:
Cranioplasty after decompressive craniectomy provides brain protection and improves cerebral hemodynamics. However, recurrent infection and sinking skin flap syndrome after cranioplasty remain cumbersome complications that require a well-planned reconstruction strategy.

Patient concerns:
A 74-year-old man presented with traumatic subdural hematoma and underwent decompressive craniectomy. Cranioplasty using an original bone flap, bone cement with wires, and a titanium mesh were complicated and resulted in recalcitrant infection and sinking skin flap syndrome.

Diagnoses:
Recurrent infection and sinking skin flap syndrome post-cranioplasty.

Interventions:
We designed a two-stage “kebab” reconstruction technique using a combination of free latissimus dorsi myocutaneous flap and delayed non-vascularized free rib graft. A well-vascularized musculocutaneous flap can obliterate dead space in skull defects and reduce bacterial inoculation in deep infections. Subsequently, delayed rib grafts act as the scaffold to expand the sunken scalp flap.

Outcomes:
At the 3-year follow-up, the patient showed improvement in headache, without evidence of surgical site infection.

Lessons:
The novel “kebab” technique using a combination of a free myocutaneous flap and delayed rib graft can eliminate bacterial growth in infected calvarial defects, reverse sinking skin flap syndrome, and minimize potential donor-site morbidity, and is therefore suitable for patients who require multiple cranioplasties and are unable to withstand major reconstructions.
The QSEN Competency Legacy Threaded Through the Entry-Level AACN Essentials
Authors: Dolansky Mary A. , Dick Tracey , Byrd Elizabeth , Miltner Rebecca S. , Layton Shannon S. 
Published: September 2023

Background:
For the last 17 years, the Quality and Safety Education for Nurses (QSEN) prelicensure competencies and knowledge, skill, and attitude (KSA) statements were integrated into nursing curricula.

Problem:
With the publication of the competency-based American Association of Colleges of Nursing (AACN) Essentials, it is important to determine the overlap of the QSEN competencies.

Approach:
We developed a QSEN-AACN prelicensure crosswalk to help faculty map and integrate the 2021 AACN Essentials into their curriculum.

Outcomes:
The 6 QSEN competencies match to the 10 AACN Essentials domains except for evidence-based practice, which is listed as a concept. Fifty graduate-level QSEN KSAs were found to better align with prelicensure education and therefore important to integrate into the crosswalk. All but 1 of the original prelicensure QSEN KSA statements and all but 2 of the 50 transferred graduate-level QSEN KSAs were found in the AACN Essentials.

Conclusion:
Faculty implementing the QSEN framework can use the QSEN-AACN competency crosswalk and QSEN teaching strategies to guide their AACN Essentials journey.
Muscle Properties, Gross Motor Performance, and Quality of Life in Children With Sickle Cell Disease
Authors: Rock Kelly , Ho Simon , Gray Vicki L. , Addison Odessa , York Teresa , Keegan Wells Diane , DeLuca Holly , Marchese Victoria 
Published: September 2023

Purpose:
To explore muscle properties, gross motor performance, and quality of life (QoL) in children with sickle cell disease (SCD) compared with controls and to assess relationships among these outcomes.

Methods:
A cross-sectional study of 24 children assessed muscle properties including: knee extension strength by dynamometry; vastus lateralis (VL) and rectus femoris (RF) muscle thickness by ultrasonography; and VL and RF neuromuscular activation (rate of muscle activation [RoA]) by electromyography (EMG). Gross motor performance and QoL were assessed by standardized tests and questionnaires.

Results:
Children with SCD had impaired knee extension strength, VL EMG RoA, gross motor performance, and QoL compared with children without SCD. Relationships among muscle properties, gross motor performance, and QoL were identified.

Conclusions:
These findings indicate that comprehensive muscle properties, gross motor performance, and QoL assessments should be considered to support and develop individualized physical therapy plans for children with SCD.
Cultural Considerations in the Formal Process of Simulation Curriculum Adaptation: A Scoping Review
Authors: Charnetski Matthew D. , Asoodar Maryam , Yu Hao , van Mook Walther 
Published: September 2023

Summary Statement
Transborder curriculum partnerships in health professions education have been increasing in numbers in recent years. These partnerships present unique challenges when transferring curricula from one context to another. It has been noted that cultural differences between institutions, faculty, staff, and learners can have profound effects on collaboration. Given the significant need for context and fidelity (especially relative to cultural considerations) in healthcare simulation education, there are gaps that need to be addressed in the transfer of these curricula. A scoping literature review was conducted examining recently published articles with relevance to simulation curriculum design or adaptation in transborder contexts to determine to what extent cultural elements are considered in the design and adaptation of simulation-based education in transborder curriculum partnerships. This review resulted in 19 studies requiring full-text review. Studies represented every region of the world with both near and distant proximity of partnering parties. From the reviewed studies, 8 categories related to curriculum adaptation were identified. These categories, when compared with the themes present in Campinha-Bacote's model of cultural competency, showed complete overlap with the 5 themes of the model plus an additional theme complementary to the model. This scoping review demonstrates that cultural considerations clearly play a role in the adaptation of simulation curricula in transborder healthcare curricular partnerships, but further research is needed to further define the exact nature of that relationship.
Mitochondrial calcium modulates odor-mediated behavioral plasticity inC. elegans
Authors: Lee Hee Kyung , Joo Dong-Kyu , Park Kyu-Sang , Yoon Kyoung-hye 
Published: September 2023

AbstractDespite growing understanding of the various roles mitochondria play in neurons, how they contribute to higher brain functions such as learning and memory remains underexplored. Here, using the nematodeCaenorhabditis elegans,we found that the mitochondrial calcium uniporter (MCU) pore forming unit MCU-1 is required for aversive learning to specific odors sensed by a single sensory neuron, AWCON. MCU-1 expression was required in the sensory neuron at the time of odor conditioning for proper behavioral response to 60 min of prolonged odor exposure. Through genetic and pharmacological manipulation, we show evidence that MCU is activated in response to prolonged odor conditioning, causing mtROS production, leading to NLP-1 secretion. Finally, we show that the timing of MCU activation and neuropeptide release correspond with the OFF-neuron properties of the AWC neuron, suggesting that mitochondrial calcium entry and neuropeptide secretion coincide with AWC activation upon odor removal. Overall, our results demonstrate that, by regulating mitochondrial calcium influx, mitochondria can modulate the synaptic response to incoming stimuli in the sensory neuron, resulting in learning and modified behavior.
Netrin signaling mediates survival of dormant epithelial ovarian cancer cells
Authors: Perampalam Pirunthan , MacDonald James I. , Zakirova Komila , Passos Daniel T. , Wasif Sumaiyah , Ramos-Valdes Yudith , Hervieu Maëva , Mehlen Patrick , Rottapel Rob , Gibert Benjamin , Correa Rohann , Shepherd Trevor G. , Dick Frederick A. 
Published: September 2023

AbstractDormancy in cancer is a clinical state in which residual disease remains undetectable for a prolonged duration. At a cellular level, rare cancer cells cease proliferation and survive chemotherapy and disseminate disease. We created a suspension culture model of high grade serous ovarian cancer (HGSOC) dormancy and devised a novel CRISPR screening approach to identify survival genes in this context. In combination with RNA-seq, we discovered the Netrin signaling pathway as critical to dormant HGSOC cell survival. We demonstrate that Netrin-1, -3, and its receptors are essential for low level ERK activation to promote survival, and that Netrin activation of ERK is unable to induce proliferation. Deletion of all UNC5 family receptors blocks Netrin signaling in HGSOC cells and compromises viability during the dormancy step of dissemination in xenograft assays. Furthermore, we demonstrate that Netrin-1 and -3 overexpression in HGSOC correlates with poor outcome. Specifically, our experiments reveal that Netrin overexpression elevates cell survival in dormant culture conditions and contributes to greater spread of disease in a xenograft model of abdominal dissemination. This study highlights Netrin signaling as a key mediator HGSOC cancer cell dormancy and metastasis.
Middle-schoolers’ reading and processing depth in response to digital and print media: An N400 study
Authors: Froud Karen , Levinson Lisa , Maddox Chaille , Smith Paul 
Published: September 2023

AbstractWe report the first use of ERP measures to identify text engagement differences when reading digitally or in print. Depth of semantic encoding is key for reading comprehension, and we predicted that deeper reading of expository texts would facilitate stronger associations with subsequently-presented related words, resulting in enhanced N400 responses to unrelated probe words and a graded attenuation of the N400 to related and moderately related words. In contrast, shallow reading would produce weaker associations between probe words and text passages, resulting in enhanced N400 responses to both moderately related and unrelated words, and an attenuated response to related words. Behavioral research has shown deeper semantic encoding of text from paper than from a screen. Hence, we predicted that the N400 would index deeper reading of text passages that were presented in print, and shallower reading of texts presented digitally.Middle-school students (n= 59) read passages in digital and print formats and high-density EEG was recorded while participants completed single-word semantic judgment tasks after each passage. Following digital text reading, the N400 response pattern anticipated for shallow reading was observed. Following print reading, the N400 response pattern expected for deeper reading was observed for related and unrelated words, although mean amplitude differences between related and moderately related probe words did not reach significance. These findings provide evidence of differences in brain responses to texts presented in print and digital media, including deeper semantic encoding for print than digital texts.
Myofibre-specific knockout of TGF-β type I receptors triggers muscle hypertrophy and promotes contraction and oxidative metabolism
Authors: Shi A. , Hillege M.M.G. , Noort W. , Offringa C. , Wu G. , Forouzanfar T. , Hoogaars W.M.H. , Wüst R.C.I. , Jaspers R.T. 
Published: September 2023

ABSTRACTTransforming growth factor-β (TGF-β) signaling is associated with progressive skeletal muscle wasting and fibrosis, while double knockout of TGF-β type I receptorsAcvr1bandTgfbr1results in hypertrophy. Gaining insights in how myofibre-specific knockout of these receptors affects muscle transcriptome, strength and mitochondrial activity could aid in the development of therapeutic interventions to improve muscle function. Here, we show that 3 months of myofibre-specific knockout of both receptors (dKO) in mice induced a 1.6-fold increase in gastrocnemius medialis mass and a 1.3-fold increase in maximal force. Soleus muscle mass and maximal force both increased 1.2-fold in dKO mice. Muscle hypertrophy in dKO mice was accompanied by a proportional increase in succinate dehydrogenase enzyme activity. Single receptor knockout caused minor phenotypical alterations. Transcriptome analyses revealed that gastrocnemius medialis had 1811 and soleus had 295 differentially expressed genes, mainly related to muscle contraction, hypertrophy, filament organization and oxidative metabolism.HgfandSlngenes were strongly upregulated in both muscles of dKO mice, whileSntb1was downregulated. This in combination of transcriptional changes are associated with muscle hypertrophy and increased mitochondrial biosynthesis. Our study highlights that myofibre-specific interference with both TGF-β type I receptors concurrently stimulates myofibre hypertrophy and mitochondrial activity.
Mitochondrial genes affect the immune response against bacterial infection (Duck Pasteurellosis) through a cascade of mechanisms mediated by nuclear immune genes-a cross talk with nuclear and mitochondrial gene
Authors: Chakraborty Argha , Pal Aruna , Debnath Manti , Sahu Jyoti , Chatterjee Paresh Nath , Pal Abantika , Mukherjee Joydeep , Samanta Rajarshi 
Published: September 2023

AbstractMitochondria possess 37 genes of its own, helpful for carrying out various functions including oxidative phosphorylation. But the mitochondria is neither independent in terms of its structure, nor its function, it depend upon nuclear gene for its functionality and replication. Our lab has earlier reported the role of mitochondrial genome in controlling health and reproduction in animal model. Reports also indicate that mitochondria have role in immune response against bacterial infection. In our current study, we depict the role of two polypeptide coding mitochondrial gene as cytochrome B and cytochrome C in providing host immunity against bacterial disease (Duck Pasteurellosis). We further observe that the mechanism was governed by cascade of mechanisms mediated through nuclear genes NRLP3, IL18 and Sting. In the first phase of the study, we have characterized Cytochrome B and Cytochrome C genes from Bengal duck and quantified through the assesment of the expression profiling with respect to healthy and Pasteurella infected ducks following a natural challenge withPasteurella multocida. In the next phase of our study, we have characterized the above nuclear genes in Bengal duck and attempted to correlate the expression with mitochondrial genes. We attempted to explore the mechanism of pathway how mitochondrial DNA triggers the nuclear immune response leading to destruction of bacterial pathogen. Molecular docking revealed how IL18 directly binds with the pathogen. This is the first report in animal model.
Sex-specific modulation of the medial prefrontal cortex by glutamatergic median raphe neurons
Authors: Collins Stuart A. , Stinson Hannah E. , Himes Amanda , Ninan Ipe 
Published: September 2023

AbstractThe current understanding of the neuromodulatory role of the median raphe nucleus (MRN) is primarily based on its putative serotonergic output. However, a significant proportion of raphe neurons are glutamatergic. The present study investigated how glutamatergic MRN input modulates the medial prefrontal cortex (mPFC), a critical component of the fear circuitry. Our studies show that VGLUT3-expressing MRN neurons modulate VGLUT3- and somatostatin-expressing neurons in the mPFC. Consistent with this modulation of mPFC GABAergic neurons, activation of MRN (VGLUT3) neurons suppresses mPFC pyramidal neuron activity and attenuates fear memory in female but not male mice. In agreement with these female-specific effects, we observed sex differences in glutamatergic transmission onto MRN (VGLUT3) neurons and mPFC (VGLUT3) neuron-mediated dual release of glutamate and GABA. Thus, our results demonstrate a cell type-specific modulation of the mPFC by MRN (VGLUT3) neurons and reveal a sex-specific role of this neuromodulation in mPFC synaptic plasticity and fear memory.
Computational exploration of the global microbiome for antibiotic discovery
Authors: Santos-Júnior Célio Dias , Torres Marcelo Der Torossian , Duan Yiqian , Rodríguez del Río Álvaro , Schmidt Thomas S.B. , Chong Hui , Fullam Anthony , Michael Kuhn , Zhu Chengkai , Houseman Amy , Somborski Jelena , Vines Anna , Zhao Xing-Ming , Bork Peer , Huerta-Cepas Jaime , de la Fuente-Nunez Cesar , Coelho Luis Pedro 
Published: September 2023

SummaryNovel antibiotics are urgently needed to combat the antibiotic-resistance crisis. We present a machine learning-based approach to predict prokaryotic antimicrobial peptides (AMPs) by leveraging a vast dataset of 63,410 metagenomes and 87,920 microbial genomes. This led to the creation of AMPSphere, a comprehensive catalog comprising 863,498 non-redundant peptides, the majority of which were previously unknown. We observed that AMP production varies by habitat, with animal-associated samples displaying the highest proportion of AMPs compared to other habitats. Furthermore, within different human-associated microbiota, strain-level differences were evident. To validate our predictions, we synthesized and experimentally tested 50 AMPs, demonstrating their efficacy against clinically relevant drug-resistant pathogens both in vitro and in vivo. These AMPs exhibited antibacterial activity by targeting the bacterial membrane. Additionally, AMPSphere provides valuable insights into the evolutionary origins of peptides. In conclusion, our approach identified AMP sequences within prokaryotic microbiomes, opening up new avenues for the discovery of antibiotics.
Concurrent stimulation of diflufenican biodegradation and changes in the active microbiome in gravel revealed by Total RNA
Authors: Ellegaard-Jensen Lea , Carvalho Pedro N. , Anwar Muhammad Zohaib , Schostag Morten Dencker , Bester Kai , Jacobsen Carsten Suhr 
Published: September 2023

AbstractThe use of slowly degrading pesticides poses a particular problem when these are applied to urban areas such as gravel paths. The urban gravel provides an environment very different from agricultural soils; i.e., it is both lower in carbon and microbial activity. We, therefore, endeavoured to stimulate the degradation of the pesticide diflufenican added to an urban gravel microcosm amended with dry alfalfa to increase microbial activity.In the present study, the formation of the primary diflufenican metabolite 2-[3-(Trifluoromethyl)phenoxy]nicotinic acid (commonly abbreviated as AE-B) was stimulated by the alfalfa amendment. The concurrent changes of the active microbial communities within the gravel were explored using shotgun metatranscriptomic sequencing of ribosomal RNA and messenger RNA. Our results showed, that while the active microbial communities in the gravel were dominated by bacteria with a relative abundance of 87.0 – 98.5 %, the eukaryotic groups, fungi and micro-eukaryotes, both had a 4-5 fold increase in relative abundance over time in the alfalfa amended treatment. Specifically, the relative abundance of microorganisms involved in degradation of complex carbon sources, Bacteroidetes, Verrucomicrobia, Sordariomycetes, Mortierellales, and Tremellales, were shown to increase in the alfalfa amended treatment. Further, the functional gene profile showed an increase in genes involved in increased activity and production of new biomass in the alfalfa treatment compared to the control, as well as pointing to genes potentially involved in biodegradation of complex carbon sources and the biotransformation of diflufenican.