bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation of the heat shock protein Tid and its involvement in stress response regulation in Apis cerana
Authors: Li Guilin , Zhang Chenghao , Wang Hongfang , Xia Wenli , Zhang Xinyi , Liu Zhenguo , Wang Ying , Zhao Hang , Xu Baohua 
Published: December 2022

Objective: The impact of various environmental stresses on native Apis cerana cerana fitness has attracted intense attention in China. However, the defence responses of A. cerana cerana to different stressors are poorly understood. Here, we aimed to elucidate the regulatory mechanism mediated by the tumorous imaginal discs (Tid) protein of A. cerana cerana (AccTid) in response to stressors.Methods: We used some bioinformatics softwares to analyse the characterisation of Tid. Then, qRT–PCR, RNA interference and heat resistance detection assays were used to explore the function of Tid in stress response in A. cerana cerana.Results:AccTid is a homologous gene of human Tid1 and Drosophila Tid56, contains a conserved J domain and belongs to the heat shock protein DnaJA subfamily. The level of AccTid induced expression was increased under temperature increases from 40°C to 43°C and 46°C, and AccTid knockdown decreased the heat resistance of A. cerana cerana, indicating that the upregulation of AccTid plays an important role when A. cerana cerana is exposed to heat stress. Interestingly, contrary to the results of heat stress treatment, the transcriptional level of AccTid was inhibited by cold, H2O2 and some agrochemical stresses and showed no significant change under ultraviolet ray and sodium arsenite stress. These results suggested that the requirement of A. cerana cerana for Tid differs markedly under different stress conditions. In addition, knockdown of AccTid increased the mRNA levels of some Hsps and antioxidant genes. The upregulation of these Hsps and antioxidant genes may be a functional complement of AccTid knockdown.Conclusion:AccTid plays a crucial role in A. cerana cerana stress responses and may mediate oxidative damage caused by various stresses. Our findings will offer fundamental knowledge for further investigations of the defence mechanism of A. cerana cerana against environmental stresses.
Bibliometric insights from publications on subchondral bone research in osteoarthritis
Authors: Wen Pengfei , Liu Rui , Wang Jun , Wang Yakang , Song Wei , Zhang Yumin 
Published: December 2022

Background: The role of subchondral bone in the pathogenesis of osteoarthritis has received continuous attention worldwide. To date, no comprehensive bibliometric analysis of this topic has been carried out. The purpose of this study was to investigate the knowledge landscape, hot spots, and research trends in subchondral bone research through bibliometrics.Methods: Web of Science Core Collection database was used to collect articles and reviews on subchondral bone in osteoarthritis published between 2003 and 2022. CiteSpace, VOSviewer, Scimago Graphica, and a bibliometric online analysis platform (http://bibliometric.com/) were used to visualize the knowledge network of countries, institutions, authors, references, and keywords in this field. Both curve fitting and statistical plotting were performed using OriginPro, while correlation analysis was done using SPSS.Results: A total of 3,545 articles and reviews were included. The number of publications on subchondral bone showed an exponential growth trend. The US produced the most (980), followed by China (862) and the United Kingdom (364). Scientific output and gross domestic product were significantly correlated (r = .948, p &lt; .001). The University of California System and Professor Pelletier Jean-Pierre were the most prolific institutions and influential authors, respectively. The most active and influential journal for subchondral bone research was Osteoarthritis and Cartilage. The majority of papers were financed by NSFC (474, 13.4%), followed by HHS (445, 12.6%), and NIH (438, 12.4%). In recent years, hot keywords have focused on the research of pathomechanisms (e.g., inflammation, apoptosis, pathogenesis, cartilage degeneration/repair, angiogenesis, TGF beta) and therapeutics (e.g., regeneration, stromal cell, mesenchymal stem cell).Conclusion: Subchondral bone research in osteoarthritis is flourishing. Current topics and next research trends would be centered on the pathomechanisms of cellular and molecular interactions in the subchondral bone microenvironment in the development of osteoarthritis and the exploration of targeted treatment medicines for the altered subchondral bone microenvironment.
ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2
Authors: Rehman Bushra , Javed Javeria , Rauf Mamoona , Khan Sumera Afzal , Arif Muhammad , Hamayun Muhammad , Gul Humaira , Khilji Sheza Ayaz , Sajid Zahoor Ahmad , Kim Won-Chan , Lee In-Jung 
Published: December 2022

IntroductionDrought has become more prevalent due to dramatic climate change worldwide. Consequently, the most compatible fungal communities collaborate to boost plant development and ecophysiological responses under environmental constraints. However, little is known about the specific interactions between non-host plants and endophytic fungal symbionts that produce growth-promoting and stress-alleviating hormones during water deficits.MethodsThe current research was rationalized and aimed at exploring the influence of the newly isolated, drought-resistant, ACC deaminase enzyme-producing endophytic fungiTrichoderma gamsii(TP),Fusarium proliferatum(TR), and its consortium (TP+TR) from a xerophytic plantCarthamus oxycanthaL. onMoringa oleiferaL. grown under water deficit induced by PEG-8000 (8% osmoticum solution).ResultsThe current findings revealed that the co-inoculation promoted a significant enhancement in growth traits such as dry weight (217%), fresh weight (123%), root length (65%), shoot length (53%), carotenoids (87%), and chlorophyll content (76%) in comparison to control plants under water deficit. Total soluble sugars (0.56%), proteins (132%), lipids (43%), flavonoids (52%), phenols (34%), proline (55%), GA3(86%), IAA (35%), AsA (170%), SA (87%), were also induced, while H2O2(-45%), ABA (-60%) and ACC level (-77%) was decreased by co-inoculation of TP and TR inM. oleiferaplants, compared with the non-inoculated plants under water deficit. The co-inoculum (TP+TR) also induced the antioxidant potential and enzyme activities POX (325%), CAT activity (166%), and AsA (21%), along with a lesser decrease (-2%) in water potential inM. oleiferaplants with co-inoculation under water deficit compared with non-inoculated control. The molecular analysis for gene expression unraveled the reduced expression of ethylene biosynthesis and signaling-related genes up to an optimal level, with an induction of antioxidant enzymatic genes by endophytic co-inoculation inM. oleiferaplants under water deficit, suggesting their role in drought stress tolerance as an essential regulatory function.ConclusionThe finding may alert scientists to consider the impacts of optimal reduction of ethylene and induction of antioxidant potential on drought stress tolerance inM. oleifera.Hence, the present study supports the use of compatible endophytic fungi to build a bipartite mutualistic symbiosis inM. oleiferanon-host plants to mitigate the negative impacts of water scarcity in arid regions throughout the world.
Psychometric validation of the Chinese version of the Adolescent Asthma Self-Efficacy Questionnaire
Authors: Wang Yuanyuan , Chen Hongyu , Cao Jinjin , Li Mei , Wang Jianan , Jing Ruochen 
Published: December 2022

BackgroundSelf-efficacy was considered as a promising target for the self-management of symptoms for adolescents with asthma. The measurement of self-efficacy in adolescents with asthma requires effective self-report tools, which have not been met with at present. So, the aim of this study was to cross-culturally validate the Adolescent Asthma Self-Efficacy Questionnaire (AASEQ).MethodsAs many as 408 adolescents with asthma were invited to take up the psychometric properties test between July 2021 and June 2022. We conducted the confirmatory factor analysis (CFA) to determine the structure of the AASEQ. The relationship between the AASEQ and General Self-Efficacy Scale was tested to evaluate the construct validity. The reliability was evaluated by retest reliability, internal consistency, and interfactor correlation.ResultsThe results of the present study showed that the confirmatory factor analysis indicated a significantly good fit for a four-factor model, which explained 62.697% of the total variance. The fit indices of the four-factor model were acceptable, and the standardized factor loading ranged from 0.631 to 0.880. The C-AASEQ showed an acceptable internal consistency (Cronbach's α = 0.810–0.927) and test-retest reliability (intraclass correlation coefficient = 0.64–0.89). Content validity index at the scale level was 0.96, and content validity index at the item level was 0.86 to 1.ConclusionThe Chinese version of Adolescent Asthma Self-Efficacy Questionnaire showed consistently acceptable positive psychometric properties and can be used as an instrument to assess the self-efficacy of adolescents with asthma in China, as corroborated in the present study.
Descriptive, injunctive, or the synergy of both? Experimenting normative information on behavioral changes under the COVID-19 pandemic
Authors: Okada Isamu , Yanagi Itaru , Kubo Yoshiaki , Kikuchi Hirokazu 
Published: December 2022

BackgroundsThe effectiveness of citizens’ behavioral changes to prevent the spread of SARS-CoV-2, such as avoiding large social events, relies on science communication from policymakers and collective action among peer citizens. Extant studies recognize the potential effects of information stimuli on citizens’ behavioral changes, including what epidemiological experts request (injunctive information) and what surrounding people behave (descriptive information). Yet, they have insufficiently assessed the co-occurrence and possible interaction of multiple information stimuli.Methods1,819 Japanese citizens aged 18 or over were recruited for an experimental survey during March 1–3, 2021 and asked their views on a hypothetical wedding attendance in Japan while being exposed to randomly assigned normative information stimuli. Their willingness to attend a wedding asked before and after the intervention was measured. Infection risk perception was also asked as a mediating variable.ResultsFindings suggest the constant supremacy of descriptive information and no synergistic effects in the interaction of multiple information stimuli. We also report that the effects of injunctive and descriptive information vary according to participants’ risk perception, age, and trust in experts.ConclusionOur experimental test enables a systematic assessment of multiple normative information and confirms the primacy of descriptive information as the main driver of behavioral change. Communication by medical experts has limitations but is still effective in specific categories of the population.
Antecedents of continuance intention in online learning systems among vocational college students: The moderating effect of gender
Authors: Li Xia , Wang Xiuling , Wei Chenming 
Published: December 2022

BackgroundThe primary objective of this study is to ascertain whether the Expectation Confirmation Model can be expanded by external variables including computer anxiety, social interaction, and self-efficacy to better understand the intention to continue using online learning systems in the post-pandemic era among vocational college students. Moreover, this research argues that the intention to continue using online learning systems among students may be gender-sensitive.MethodsThe researchers surveyed 482 students from eight vocational colleges in Jiangxi Province using a structured questionnaire. Partial Least Squares Structural equation modeling is used to verify the research model.ResultsThe outcomes demonstrate that the proposed model adequately explains the continuous use intention for online learning systems at a 76.6% confidence level. All of the newly introduced variables in the ECM are shown to be significant and relevant to explicate continuous use intention. Our survey results show that gender differences in intention to continue using online learning systems exist objectively, but this difference is not a natural difference.ConclusionThis research fills a void in the current literature on online learning and probes into how learning may be made more long-lasting in intricate environments.
Links between mothers’ ACEs, their psychopathology and parenting, and their children’s behavior problems–A mediation model
Authors: Hanetz-Gamliel Keren , Dollberg Daphna G. 
Published: December 2022

IntroductionChildren of mothers with a history of adverse childhoods are at greater risk of behavior problems. However, the mechanisms through which a mother’s early adverse experiences (ACEs) are transmitted to her children need further study. Our goal was to examine a conceptual mediational model linking mothers’ ACEs, maternal psychopathology symptoms, and parenting behaviors with children’s internalizing and externalizing behaviors sequentially.MethodsA sample of 153 Israeli mothers of children ages 3-12 (52% girls) participated in the study, and most of the mothers (94.7%) were cohabiting with a spouse. Mothers completed online questionnaires about their early adverse experiences, psychopathology symptoms, parenting behavior, and their children’s internalizing and externalizing behavior.ResultsResults showed that mothers with higher ACE scores reported more maternal psychopathology symptoms and more internalizing behavior in their children. The mother’s psychopathology in and of itself mediated the link between her ACEs and her child’s internalizing and externalizing behavior. Moreover, an indirect sequential path emerged linking ACEs with the mother’s psychopathology symptoms, which, in return, were linked with hostile parenting. Hostile parenting, in turn, was linked with children’s internalizing and externalizing behavior.DiscussionThese findings highlight the complicated and intertwined ways in which adverse experiences early in the mother’s life might put her child’s wellbeing at risk. The findings suggest that ACEs are linked to maternal affect dysregulation, which interferes with parenting, increasing the risk of behavior problems in children. The findings underscore the need to assess mothers’ adverse history, psychological distress, and parenting behavior, and provide treatments that can reduce the intergenerational transmission of early adverse experiences.
Analysis of the utilization, processes, and outcomes of inpatient mental healthcare during the first three waves of the COVID-19 pandemic in the federal state of North Rhine-Westphalia, Germany
Authors: Zielasek Jürgen , Lehmann Isabell , Vrinssen Jürgen , Gouzoulis-Mayfrank Euphrosyne 
Published: December 2022

BackgroundDuring the first phase of the Coronavirus-19 disorder (COVID-19) pandemic in the spring of 2020, utilization of inpatient mental healthcare was significantly reduced. We now report on a long-term observational study of inpatient mental healthcare in a large psychiatric hospital association in North Rhine-Westphalia, Germany, covering the second and third pandemic waves of autumn and winter 2020 followed up until June 2021.ObjectivesAnalysis of the changes of inpatient and day patient mental healthcare utilization in an association of psychiatric hospitals during the COVID-19 pandemic from January 2020 until June 2021.Materials and methodsWe used the statistics database of the association of the nine psychiatric hospitals of the Rhineland Regional Council (Landschaftsverband Rheinland, LVR). We compared the case numbers of the pandemic period with previous years and analyzed changes in the diagnostic spectrum, rates of coercion and therapeutic outcomes. We also analyzed age, gender, diagnoses and coercive measures of patients tested positive for COVID-19 during inpatient psychiatric healthcare.ResultsCase rates were reduced during and after the COVID-19 pandemic episodes of 2020 and the following months of spring and summer 2021. Changes varied between diagnostic groups, and there were even increases of case numbers for acute psychotic disorders. Coercive measures increased during the pandemic, but therapeutic outcomes were maintained at the pre-pandemic level. Women and patients of higher ages were overrepresented among psychiatric inpatients with COVID-19.ConclusionThe COVID-19 pandemic led to over during reductions of inpatient psychiatric hospital admissions and changes of the diagnostic spectrum accompanied by increased rates of coercive measures. These effects may reflect an overall increased severity of mental disorders during the COVID-19 pandemic, deferrals of inpatient admissions or a lack of outpatient mental healthcare services utilization. To differentiate and quantitate these potential factors, further studies in the general population and in the different mental healthcare sectors are needed. In order to reduce the number of COVID-19 cases in psychiatric hospitals, vaccination of people of higher ages and with dementias seem to be the most needed strategy.
Work-related stress of companies' directors during the first lockdown due to the COVID-19
Authors: Dutheil Frédéric , Jeanton Carole , Vilmant Audrey , Lambert Céline , Clinchamps Maelys , Ugbolue Ukadike Chris , Schmidt Jeannot , Vallet Guillaume T. , Bouillon-Minois Jean-Baptiste 
Published: December 2022

BackgroundThe COVID-19 pandemic and the first lockdown were particularly stressful with a major economic impact, but the impact on stress of company directors was not known. Therefore, this study aimed to assess that impact and the characteristics of companies the most at risk.MethodA online questionnaire was sent to 13,114 company. It assessed stress at work, number of employees, sector of activity, business activity rate and geographical location. It studied the mean stress levels, the percentage of stress &gt; 8/10 and carried out an analysis of the characteristics of the most at-risk companies.ResultsA total of 807 company directors responded. Their stress levels increased by 25.9% during lockdown and 28.7% of them had a stress &gt; 8/10. Sectors which had the biggest increase in stress levels during lockdown were retail trade, wholesale trade, and nursing homes. Sectors the most at risk of stress &gt;8/10 during lockdown tended to be nursing homes, pharmacies, and IT activities. Biggest companies had the highest increase in stress levels.ConclusionThe first lockdown of the COVID-19 pandemic had a major impact on the stress of company directors. Directors of large companies were the most exposed to stress as well as medical and IT activities.
Associations and pathways between residential greenness and metabolic syndromes in Fujian Province
Authors: Li Xiaoqing , Wang Qinjian , Feng Chuanteng , Yu Bin , Lin Xi , Fu Yao , Dong Shu , Qiu Ge , Jin Aik Darren How , Yin Yanrong , Xia Pincang , Huang Shaofen , Liu Nian , Lin Xiuquan , Zhang Yefa , Fang Xin , Zhong Wenling , Jia Peng , Yang Shujuan 
Published: December 2022

BackgroundGreenness exposure is beneficial to human health, but its potential mechanisms through which the risk for metabolic syndrome (MetS) could be reduced have been poorly studied. We aimed to estimate the greenness-MetS association in southeast China and investigate the independent and joint mediation effects of physical activity (PA), body mass index (BMI), and air pollutants on the association.MethodsA cross-sectional study was conducted among the 38,288 adults based on the Fujian Behavior and Disease Surveillance (FBDS), established in 2018. MetS was defined as the presence of three or more of the five components: abdominal obesity, elevated triglyceride, reduced high-density lipoprotein cholesterol (HDL-C), high blood pressure, and elevated fasting glucose. The residential greenness exposure was measured as the 3-year mean values of the normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI) within the 250, 500, and 1,000 meters (m) buffer zones around the residential address of each participant. Logistic regression models were used to estimate the greenness-MetS association. The causal mediation analysis was used to estimate the independent and joint mediation effects of PA, BMI, particulate matter with an aerodynamic diameter of 2.5 μm (PM2.5), particulate matter with an aerodynamic diameter ≤ 10 μm (PM10), nitrogen dioxide (NO2), and sulfur dioxide (SO2).ResultsEach interquartile range (IQR) increase in greenness was associated with a decrease of 13% (OR = 0.87 [95%CI: 0.83, 0.92] for NDVI500m and OR = 0.87 [95%CI: 0.82, 0.91] for EVI500m) in MetS risk after adjusting for covariates. This association was stronger in those aged &lt; 60 years (e.g., OR = 0.86 [95%CI: 0.81, 0.92] for NDVI500m), males (e.g., OR = 0.73 [95%CI: 0.67, 0.80] for NDVI500m), having an educational level of primary school or above (OR = 0.81 [95%CI: 0.74, 0.89] for NDVI500m), married/cohabitation (OR = 0.86 [95%CI: 0.81, 0.91] for NDVI500m), businessman (OR = 0.82 [95%CI: 0.68, 0.99] for NDVI500m), other laborers (OR = 0.77 [95%CI: 0.68, 0.88] for NDVI500m), and non-smokers (OR = 0.77 [95%CI: 0.70, 0.85] for NDVI500m). The joint effect of all six mediators mediated about 48.1% and 44.6% of the total effect of NDVI500m and EVI500m on the MetS risk, respectively. Among them, BMI showed the strongest independent mediation effect (25.0% for NDVI500m), followed by NO2 and PM10.ConclusionExposure to residential greenness was associated with a decreased risk for MetS. PA, BMI, and the four air pollutants jointly interpreted nearly half of the mediation effects on the greenness-MetS association.
Typology of Iranian farmers' vulnerability to the COVID-19 outbreak
Authors: Moradhaseli Somayeh , Ataei Pouria , Karimi Hamid , Hajialiani Sara 
Published: December 2022

ContextPresently, farmers are faced with a new crisis caused by the outbreak of COVID-19. On the one hand, they are vulnerable to such respiratory diseases due to the nature of their farming activity. On the other hand, they will definitely be influenced by the pandemic in different aspects no matter if they do not contract the infection. So, this research aimed to study the vulnerability of farmers to the COVID-19 pandemic.MethodsThe present study was conducted using the quantitative approach and a descriptive-survey methodology. The statistical population was composed of farmers in Kermanshah province, Iran (N= 126,900). The sample (n= 382) was taken by the multistage stratified sampling technique with proportional allocation. The research instrument was a self-designed questionnaire whose face and content validity was confirmed by a panel of relevant experts and its reliability was supported in a pilot test.ResultsThe main damages of the pandemic to the farmers were found to be the increased costs of production, permanent or seasonal unemployment, reduced access to crop sale markets, and reduced control over pests and diseases at farms. The results revealed that the means of environmental, agronomic-vocational, and economic vulnerability were greater than the scale mean. The results also illustrated significant differences in the means of economic, psychological-social, agronomic-vocational, and environmental dimensions of vulnerability. Among these dimensions, the variable of agronomic-vocational vulnerability had the highest mean, and the variable of psychological-social vulnerability had the lowest mean.ConclusionFarmers have been one of the groups most severely influenced and damaged by the pandemic in various aspects. In this regard, organizations and institutions in charge of different agriculture sections, especially the Office of Agricultural Extension and Education, must develop practical strategies to reduce the effect of the pandemic on the agricultural sector. Identifying the dimensions and parameters of farmers' vulnerability in the face of COVID-19 can provide new and appropriate solutions to relevant planners and policymakers.