bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of the 2019/2020 Australian landscape fires on infant feeding and contaminants in breast milk in women with asthma
Authors: Beyene Tesfalidet , Zosky Graeme R. , Gibson Peter G. , McDonald Vanessa M. , Holliday Elizabeth G. , Horvat Jay C. , Vertigan Anne E. , Van Buskirk Joe , Morgan Geoffrey G. , Jegasothy Edward , Hanigan Ivan , Murphy Vanessa E. , Jensen Megan E. 
Published: February 2023

Abstract
Background
The 2019/2020 Australian landscape fires (bushfires) resulted in prolonged extreme air pollution; little is known about the effects on breastfeeding women and their infants. This study aimed to examine the impact of prolonged landscape fires on infant feeding methods and assess the concentration of polycyclic aromatic hydrocarbons (PAHs) and elements in breast milk samples.

Methods
From May – December 2020, women with asthma, who were feeding their infants during the fires, were recruited from an existing cohort. Data on infant feeding and maternal concern during the fires were retrospectively collected. Breast milk samples were collected from a sample of women during the fire period and compared with samples collected outside of the fire period for levels of 16 PAHs (gas chromatography coupled with mass spectrometry), and 20 elements (inductively coupled plasma-mass spectrometry).

Results
One-hundred-and-two women who were feeding infants completed the survey, and 77 provided 92 breast milk samples. Two women reported concern about the impact of fire events on their infant feeding method, while four reported the events influenced their decision. PAHs were detected in 34% of samples collected during, versus no samples collected outside, the fire period (cross-sectional analysis); specifically, fluoranthene (median concentration 0.015 mg/kg) and pyrene (median concentration 0.008 mg/kg) were detected. Women whose samples contained fluoranthene and pyrene were exposed to higher levels of fire-related fine particulate matter and more fire days, versus women whose samples had no detectable fluoranthene and pyrene. Calcium, potassium, magnesium, sodium, sulphur, and copper were detected in all samples. No samples contained chromium, lead, nickel, barium, or aluminium. No statistically significant difference was observed in the concentration of elements between samples collected during the fire period versus outside the fire period.

Conclusions
Few women had concerns about the impact of fire events on infant feeding. Detection of fluoranthene and pyrene in breast milk samples was more likely during the 2019/2020 Australian fire period; however, levels detected were much lower than levels expected to be related to adverse health outcomes.
Consensus guidelines for diagnosis and management of anemia in epidermolysis bullosa
Authors: Liy-Wong Carmen , Tarango Cristina , Pope Elena , Coates Thomas , Bruckner Anna L. , Feinstein James A. , Schwieger-Briel Agnes , Hubbard Lynne D. , Jane Clapham , Torres-Pradilla Mauricio , Zmazek Matija , Lara-Corrales Irene 
Published: February 2023

Abstract
Background
Anemia is a common complication of severe forms of epidermolysis bullosa (EB). To date, there are no guidelines outlining best clinical practices to manage anemia in the EB population. The objective of this manuscript is to present the first consensus guidelines for the diagnosis and management of anemia in EB.

Results
Due to the lack of high-quality evidence, a consensus methodology was followed. An initial survey exploring patient preferences, concerns and symptoms related to anemia was sent to EB patients and their family members. A second survey was distributed to EB experts and focused on screening, diagnosis, monitoring and management of anemia in the different types of EB. Information from these surveys was collated and used by the panel to generate 26 consensus statements. Consensus statements were sent to healthcare providers that care for EB patients through EB-Clinet. Statements that received more than 70% approval (completely agree/agree) were adopted.

Conclusions
The end result was a series of 6 recommendations which include 20 statements that will help guide management of anemia in EB patients. In patients with moderate to severe forms of EB, the minimum desirable level of Hb is 100 g/L. Treatment should be individualized. Dietary measures should be offered as part of management of anemia in all EB patients, oral iron supplementation should be used for mild anemia; while iron infusion is reserved for moderate to severe anemia, if Hb levels of > 80–100 g/L (8–10 g/dL) and symptomatic; and transfusion should be administered if Hb is < 80 g/L (8 g/dL) in adults and < 60 g/L (6 g/dL) in children.
Synergistic improvement of cinnamylamine production by metabolic regulation
Authors: Yuan Shan , Xu Chao , Jin Miaomiao , Xian Mo , Liu Wei 
Published: February 2023

Abstract
Background
Aromatic primary amines (APAs) are key intermediates in the chemical industry with numerous applications. Efficient and mild biocatalytic synthesis is an excellent complement to traditional chemical synthesis. Our lab previously reported a whole-cell catalytic system for the synthesis of APAs catalyzed by carboxylic acid reductase from Neurospora crassa (ncCAR) and ω-transaminase from Ochrobactrum anthropi (OATA). However, the accumulation of toxic intermediates (aromatic aldehydes) during biocatalytic synthesis affected yields of APAs due to metabolic imbalance.

Results
In this work, the biocatalytic synthesis of APAs (taking cinnamylamine as an example) was metabolically regulated by the overexpression or knockout of five native global transcription factors (TFs), the overexpression of eight native resistance genes, and optimization of promoters. Transcriptome analysis showed that knockout of the TF arcA increased the fluxes of NADPH and ATP in E. coli, while the rate of pyruvate metabolism was accelerated. In addition, the genes related to stress and detoxification were upregulated with the overexpression of resistance gene marA, which reduced the NADPH level in E. coli. Then, the expression level of soluble OATA increased by promoter optimization. Overall, arcA and marA could regulate the catalytic rate of NADPH- dependent ncCAR, while arcA and optimized promoter could regulate the catalytic rate of OATA. Lastly, the cinnamylamine yield of the best metabolically engineered strain S020 was increased to 90% (9 mM, 1.2 g/L), and the accumulation of cinnamaldehyde was below 0.9 mM. This work reported the highest production of cinnamylamine by biocatalytic synthesis.

Conclusion
This regulatory process provides a common strategy for regulating the biocatalytic synthesis of other APAs. Being entirely biocatalytic, our one-pot procedure provides considerable advantages in terms of environmental and safety impacts over reported chemical methods.
Case management and care expertise as a prevention approach for adults with intellectual disabilities (FaPP-MgB): study protocol for a randomized-controlled trial
Authors: Nadolny Stephan , Bruland Dirk , Grunwald Marie , Gröndahl Annika , Grammatico Jessica , Richter Miriam Tariba , Grebe Christian , Latteck Änne-Dörte 
Published: February 2023

Abstract
Background
Adults with intellectual disabilities have a higher prevalence of unhealthy eating habits, stress, low levels of mobility, and comparable drug consumption as the general population. Consequently, they suffer from several chronic diseases earlier and more often, but there are fewer prevention and health promotion services including this population. The goal of this study is to determine if an advanced practice nursing approach in the community with home visits is an effective way to improve the health status of adults with intellectual disabilities.

Methods
We will conduct a randomized-controlled trial with waiting list design in Hamburg, Germany. Inclusion criteria are diagnosis ICD F70-F79 and exclusion criteria are care level > 3 according to the German Social Code XI or being at the end-of-life. Participants will be block randomized. The intervention consists of advanced practice nurses performing case management, social space analysis, prevention planning, and counseling through four outreach home visits on nutrition, mobility, addiction, and stress. Comparison is usual care. The primary outcome is health status (WHODAS) after 12 months. Secondary outcomes are health-related quality of life (EQ-5D) and resilience (RS-11) after 6 and 12 months. The calculated sample size is 256 with an estimated dropout of 30%. Raters and analysts will be blinded. Analysis will be performed using ANCOVAs.

Discussion
By providing case management and utilizing their nursing expertise, advanced practice nurses will provide valuable input and guidance on prevention and health promotion for people with intellectual disabilities. They will close the gap between health and social care, which is prominent in Germany, through cooperation between the existing care sectors.
The findings will be disseminated in peer-reviewed journals and presented at national and international conferences.

Trial registration
German Clinical Trials Register, DRKS00028771, registered 4 July 2022, Universal Trial Number: U1111-1277–0595.
Comparison of diabetes distress and depression screening results of emerging adults with type 1 diabetes onset at different ages: findings from the German early-onset T1D study and the German Diabetes Study (GDS)
Authors: Stahl-Pehe Anna , Bächle Christina , Bódis Kálmán , Zaharia Oana-Patricia , Lange Karin , Holl Reinhard W. , Roden Michael , Rosenbauer Joachim ,   for the GDS Group, Roden M. , Al-Hasani H. , Belgardt B , Bönhof GJ. , Burkart V , Buyken A. E. , Geerling G. , Herder C. , Icks A. , Jandeleit-Dahm K. , Kotzka J. , Kuß O. , Lammert E. , Rathmann W. , Schrauwen-Hinderling V. B. , Szendroedi J. , Trenkamp S. , Wagner R. 
Published: February 2023

AbstractBackgroundDiabetes distress is increasingly considered one of the most important psychosocial issues in the care of people with type 1 diabetes (T1D). We analyse whether diabetes distress and depression screening results of emerging adults are associated with the age at T1D onset.MethodsData were taken from two cohort studies conducted at the German Diabetes Center, Düsseldorf, Germany. The 18–30-year-old participants had an age at onset either before the age of 5 years (childhood-onset long-term T1D study group, N = 749) or during adulthood (adult-onset short-term T1D study group from the German Diabetes Study (GDS), N = 163). Diabetes distress and depression screening were analysed by means of the 20-item Problem Areas in Diabetes (PAID-20) scale and the nine-item depression module from the Patient Health Questionnaire (PHQ-9). The average causal effect of age at onset was estimated by a doubly robust causal inference method.ResultsThe PAID-20 total scores were increased in the adult-onset study group [potential outcome mean (POM) 32.1 (95% confidence interval 28.0; 36.1) points] compared to the childhood-onset study group [POM 21.0 (19.6; 22.4) points, difference 11.1 (6.9; 15.3) points, p<0.001] adjusted for age, sex and haemoglobin A1c (HbA1c) levels. Moreover, more participants in the adult-onset group [POM 34.5 (24.9; 44.2) %] than in the childhood-onset group [POM 16.3 (13.3; 19.2) %] screened positive for diabetes distress [adjusted difference 18.3 (8.3; 28.2) %, p<0.001]. The PHQ-9 total score [difference 0.3 (-1.1; 1.7) points, p=0.660] and the proportion of participants with a positive screening result for depression [difference 0.0 (-12.7; 12.8) %, p=0.994] did not differ between the groups in the adjusted analyses.ConclusionsEmerging adults with short-term type 1 diabetes screened positive for diabetes distress more often than adults with type 1 diabetes onset during early childhood when age, sex and HbA1c values were considered confounding factors. Accounting for age at onset or the duration of diabetes may help explain the heterogeneity in the data when psychological factors are examined.
Prevalence of overweight/obesity and its relationship with metabolic syndrome and fatty liver index in adult patients with type 1 diabetes. A Brazilian multicenter study
Authors: Gomes Marilia Brito , Santos Deborah Conte , Drummond Karla , Pinheiro André , Muniz Luiza Harcar , Leal Franz , Negrato Carlos Antonio ,   Brazilian Type 1 Diabetes Study Group (BrazDiab1SG), Melo Laura Nunes , Cobas Roberta , Tannus Lucianne Righeti Monteiro , Rodacki Melanie , Zajdenverg Lenita , Dantas Joana Rodrigues , Corrêa-Giannella Maria Lúcia Cardillo , Admoni Sharon Nina , Santos Daniele Pereirados , Guedes Mariade Fatima , Dib Sergio Atala , Filho Celso Ferreirde Camargo Sallum , Pavin Elisabeth João , Takano Caroline , Rea Rosângela Roginski , Romanzini Nicole Balster , Azevedo Mirela , Canani Luis Henrique , Pedrosa Hermelinda Cordeiro , Tolentino Monica , Aguiar Cejana Hamu , Fonseca Reine Marie Chaves , Fonseca Ludmila Chaves , Kasprowicz Raffaele , Forti Adriana Costa e , Mendes Angela Delmira Nunes , Junior Renan Montenegro , Fernandes Virgínia Oliveira , Felício João Soares , Santos Flavia Marques 
Published: February 2023

Abstract
Aims
To determine the prevalence of overweight/obesity and its relationship with metabolic syndrome (MS), fatty liver index (FLI), cardiovascular risk factors (CVRF), and diabetes-related chronic complications (DRCC) in adult patients with type 1 diabetes (T1D).

Methods
This study was conducted in 14 Brazilian public clinics in ten cities, with 1,390 patients: 802 females (57.7%), 779 (56.0%) Caucasians, aged 33.6 ± 10.8 years, age at diagnosis, 16.2 ± 9.2 years, diabetes duration, 17.4 ± 9.2 years, and HbA1c 8.8 ± 2.0%.

Results
Overall, 825 patients (59.4%) had normal weight, and 565 had overweight/obesity; ( 429 (30.9%) presented overweight and 136 (9.8%) presented obesity). After adjustments, overweight/obesity was associated with age, family history of overweight/obesity, total daily insulin dose, hypertension, adherence to diet, type of health care insurance, use of metformin, levels of C-reactive protein, triglycerides, uric acid and HDL-cholesterol. These patients also presented a higher prevalence of MS, FLI ≥ 60, and CVRF than patients without overweight/obesity. Overweight/obesity was not associated with DRCC and with HbA1c levels.

Conclusions
Patients with T1D with overweight/obesity presented traditional risk factors for DRCC, cardiovascular diseases, MS, and non-alcoholic fatty liver disease; most of these risk factors are modifiable and can be avoided with interventions that prevent overweight/obesity.
An overdetermined problem of the biharmonic operator on Riemannian manifolds
Authors: Chen Fan , Huang Qin , Ruan Qihua 
Published: February 2023

AbstractLet $(M,g)$
(
M
,
g
)
be an n-dimensional complete Riemannian manifold with nonnegative Ricci curvature. In this paper, we consider an overdetermined problem of the biharmonic operator on a bounded smooth domain Ω in M. We deduce that the overdetermined problem has a solution only if Ω is isometric to a ball in $\mathbb{R}^{n}$

R
n

. Our method is based on using a P-function and the maximum principle argument. This result is a generalization of the overdetermined problem for the biharmonic equation in Euclidean space.
Immune-associated biomarkers identification for diagnosing carotid plaque progression with uremia through systematical bioinformatics and machine learning analysis
Authors: Liu Chunjiang , Tang Liming , Zhou Yue , Tang Xiaoqi , Zhang Gang , Zhu Qin , Zhou Yufei 
Published: February 2023

Abstract
Background
Uremia is one of the most challenging problems in medicine and an increasing public health issue worldwide. Patients with uremia suffer from accelerated atherosclerosis, and atherosclerosis progression may trigger plaque instability and clinical events. As a result, cardiovascular and cerebrovascular complications are more likely to occur. This study aimed to identify diagnostic biomarkers in uremic patients with unstable carotid plaques (USCPs).

Methods
Four microarray datasets (GSE37171, GSE41571, GSE163154, and GSE28829) were downloaded from the NCBI Gene Expression Omnibus database. The Limma package was used to identify differentially expressed genes (DEGs) in uremia and USCP. Weighted gene co-expression network analysis (WGCNA) was used to determine the respective significant module genes associated with uremia and USCP. Moreover, a protein–protein interaction (PPI) network and three machine learning algorithms were applied to detect potential diagnostic genes. Subsequently, a nomogram and a receiver operating characteristic curve (ROC) were plotted to diagnose USCP with uremia. Finally, immune cell infiltrations were further analyzed.

Results
Using the Limma package and WGCNA, the intersection of 2795 uremia-related DEGs and 1127 USCP-related DEGs yielded 99 uremia-related DEGs in USCP. 20 genes were selected as candidate hub genes via PPI network construction. Based on the intersection of genes from the three machine learning algorithms, three hub genes (FGR, LCP1, and C5AR1) were identified and used to establish a nomogram that displayed a high diagnostic performance (AUC: 0.989, 95% CI 0.971–1.000). Dysregulated immune cell infiltrations were observed in USCP, showing positive correlations with the three hub genes.

Conclusion
The current study systematically identified three candidate hub genes (FGR, LCP1, and C5AR1) and established a nomogram to assist in diagnosing USCP with uremia using various bioinformatic analyses and machine learning algorithms. Herein, the findings provide a foothold for future studies on potential diagnostic candidate genes for USCP in uremic patients. Additionally, immune cell infiltration analysis revealed that the dysregulated immune cell proportions were identified, and macrophages could have a critical role in USCP pathogenesis.
Analysis of clinical and genomic profiles of therapy-related myeloid neoplasm in Korea
Authors: Yun Jiwon , Song Hyojin , Kim Sung-Min , Kim Soonok , Kwon Seok Ryun , Lee Young Eun , Jeong Dajeong , Park Jae Hyeon , Kwon Sunghoon , Yun Hongseok , Lee Dong Soon 
Published: February 2023

AbstractBackgroundTherapy-related myeloid neoplasm (T-MN) rarely occurs among cancer survivors, and was characterized by poor prognosis. T-MN has germline predisposition in a considerable proportion. Here, clinical characteristics and germline/somatic variant profiles in T-MN patients were investigated, and the findings were compared with those of previous studies.MethodsA review of medical records, cytogenetic study, targeted sequencing by next-generation sequencing, and survival analysis were performed on 53 patients with T-MN at a single institution in Korea.ResultsThe patients were relatively younger compared to T-MN patients in other studies. Our T-MN patients showed a high frequency of complex karyotypes, −5/del(5q), and −7/del(7q), which was similar to the Japanese study group but higher than the Australian study group. The most common primary disease was non-Hodgkin lymphoma, followed by breast cancer. The detailed distributions of primary diseases were different across study groups. Seven patients (13.2%) harbored deleterious presumed/potential germline variants in cancer predisposition genes (CPG) such asBRIP1,CEBPA,DDX41,FANCM,NBN,NF1, andRUNX1. In the somatic variant profile,TP53was the most frequently mutated gene, which was consistent with the previous studies about T-MN. However, the somatic variant frequency in our study group was lower than in other studies. Adverse factors for overall survival were male sex, older age, history of previous radiotherapy, previous longer cytotoxic therapy, and −5/del(5q).ConclusionThe findings of our study corroborate important information about T-MN patients. As well as a considerable predisposition to CPG, the clinical characteristics and somatic variant profile showed distinctive patterns. Germline variant testing should be recommended for T-MN patients. If the T-MN patients harbor pathogenic germline variants, the family members for stem cell donation should be screened for carrier status through germline variant testing to avoid donor-derived myeloid neoplasm. For the prediction of the prognosis in T-MN patients, sex, age, past treatment history, and cytogenetic findings can be considered.
It’s not all about control: challenging mainstream framing of eating disorders
Authors: Branley-Bell Dawn , Talbot Catherine V. , Downs James , Figueras Carolina , Green Jessica , McGilley Beth , Murphy-Morgan Claire 
Published: February 2023

Abstract
Background
The concept of control has long been suggested as a central factor in eating disorder (ED) aetiology. The concept is now so mainstream that it risks being used in a potentially reductionist, stigmatising or otherwise harmful manner. In this paper, we explore and discuss our positions on the use of control-related terminology for EDs.

Methods
The authors of this auto-ethnographic position paper include academic researchers, individuals with lived experience and clinicians (not mutually exclusive). In sharing our experiences and observations, we aim to raise awareness of the wider impacts that control framing can have on ED perceptions, treatment, recovery and individuals’ lived experience.

Results
We argue that although control can play a role in some ED experiences, an overemphasis upon this factor to the exclusion of other conceptualisations is not beneficial.

Conclusions
To mitigate against pathologisation of an individual, it is important to challenge a discourse that can lead to EDs being perceived as something ‘wrong’ with the individual, rather than a consequence of life events or other environmental influences. We identify priorities for the future for researchers, clinicians, policy makers and the wider public.
Seasonal mixed layer temperature and salt balances in the Banda Sea observed by an Argo float
Authors: Ismail Mochamad Furqon Azis , Karstensen Johannes , Ribbe Joachim , Arifin Taslim , Chandra Handy , Akhwady Rudhy , Yulihastin Erma , Basit Abdul , Budiman Asep Sandra 
Published: February 2023

AbstractFor the first time, the processes controlling the variations of mixed layer temperature (MLT) and salinity (MLS) in the Banda Sea are quantified using data from a single Argo float in combination with satellite and reanalysis outputs from August 2017 to August 2019. This augments previous studies that utilized ocean model data only. We document the presence of a barrier layer and quantify the roles of air-sea heat and mass exchanges, horizontal advection, and vertical entrainment in the seasonal variability of MLT and MLS. We find that heat gains and losses at the air-sea interface are the main contributor to the warming and cooling of the MLT. Seasonal changes in MLS are driven by advection of low salinity water rather than freshwater fluxes from precipitation and evaporation. This is particularly the case during the late northwest and monsoon transition period from February to April when low salinity is advected eastward from the Java Sea into the Banda Sea.