bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired bioenergetic response mediates the antifungal effect of lipopeptides from Bacillus tequilensis EA-CB0015 and cinnamon extract against Fusarium oxysporum f. sp. cubense
Authors: Ramírez-Mejía Julieta , Villegas-Escobar Valeska , Gómez Luis A 
Published: June 2023

Abstract
Lipopeptides (LPs) from B. tequilensis EA-CB0015 have antifungal activity against Fusarium species. Specifically, against F. oxysporum f. sp. cubense (Foc), the cause of the Fusarium wilt of banana. In addition, cinnamon (CN) extract is also known for its inhibitory activity against the Fusarium genus. The main goal of this research was to determine if the effect of LPs, CN extract or their combination against Foc is related to an impairment of mitochondrial function. Our results show that biomass decreased by 74% (p < 0.0001) and 84% (p < 0.0001) when cultures were treated with 128 ppm LPs and 152.5 ppm CN extract, respectively. In parallel, we found a pronounced impairment of the bioenergetic response. That is, the routine oxygen consumption rate diminished by 55% (p = 0.0148) and 38% (p < 0.0001), respectively. Moreover, the ATP-linked respiratory rate decreased by 63% (p = 0.0461) and 44% (p = 0.0005), while the FCCP-simulated respiratory rate by 63% (p = 0.0255) and 45% (p = 0.0002). Therefore, our data suggest that the altered bioenergetic response observed in cultures of Foc treated with LPs or CN is mainly caused by an impairment of the activity of the ETC components. On the other hand, biomass production was reduced by 80% (p < 0.0001) when cultures were treated with a mixture comprising only 10% LPs and 40% CN extract. Furthermore, ATP-linked and FCCP-stimulated respiratory rates decreased by 62% (p = 0.0024) and 68% (p < 0.0001), respectively under the same conditions. A potentially synergistic antifungal effect of cyclic LPs with a CN extract is suggested.
Effects of low-dose intravenous heparin therapy in aneurysmal subarachnoid hemorrhage: a randomized controlled clinical trial protocol
Authors: Zhang Yifan , Hu Jiangang 
Published: June 2023

Abstract
Background
Heparin anticoagulation therapy is a widely used method to prevent cerebral vasospasm (CV) and venous thrombosis in patients with subarachnoid hemorrhage caused by ruptured cerebral aneurysms. Subcutaneous heparin injection is considered safe and effective, whereas continuous intravenous heparin infusion is still being debated due to the risk of bleeding. Although most retrospective studies have confirmed the safety and effectiveness of unfractionated heparin (UFH) after aneurysm embolization therapy and its ability to reduce CV, there is still no randomized clinical trial comparing UFH and subcutaneous low-molecular-weight heparin (LMWH) injection in this population. This study aims to Therefore, this study aims to compare the clinical outcomes associated with these two treatment approaches.
Methods
The study is an open-label, single-center, randomized controlled trial and aims to recruit 456 patients, with 228 patients in each group. The primary outcome was CV, the second outcomes measures are occurrence of bleeding events, ischemic events, heparin-induced thrombocytopenia, deep vein thrombosis, cerebral venous circulation time, brain edema score, and hydrocephalus incidence.
Ethics and dissemination:
This study protocol obtained ethical approval from the Ethics Committee of Baoan People’s Hospital, Shenzhen, Guangdong (approval number: BYL20220805). This work will be published in peer-reviewed international medical journals and presented at medical conferences.
ClinicalTrails ID:
NCT05696639. Registered on March 30, 2023.
Analysis of factors influencing IVF-ET treatment outcome in infertile women and construction of a predictive model
Authors: Zhang Pan , Pan Congcong , Chen Yao , Zhong Ni , Zhang Ao , Chen Shengjia , Pan Haiyan , Lin Bing 
Published: June 2023

Abstract
Objective
To construct a predictive model for cumulative live birth rates(CLBRs) in patients treated with IVF-ET,develop a nomogram for individual prediction of CLBRs.
Methods
Patients who underwent embryo transfer in our hospital from 2017–2021 were selected for retrospective analysis, and the data were randomly divided into modelling group and validation group according to 7:3. The CLBRs nomogram was constructed using the modelling group, and the data of the validation group for internal verification. The discrimination and calibration of the predictive model is assessed by calculating the C-index and plotting the calibration curve of the model.
Results
A total of 2037 cases were included in the sample data, of which 1425 cases were in the modelling group and 612 cases in the validation group.Multi-factor logistic regression analysis was carried out on the characteristic indicators screened by the univariate analysis.The
Realistic representation of mixed-phase clouds increases future climate warming
Authors: Hofer Stefan , Hahn Lily , Shaw Jonah , McGraw Zachary , Bruno Olimpia , Hellmuth Franziska , Pietschnig Marianne , Mostue Idunn , David Robert , Carlsen Tim , Storelvmo Trude 
Published: June 2023

Abstract

Clouds are the main source of uncertainties when projecting climate change. Mixed-phase clouds (MPCs) that contain ice and supercooled-liquid particles are especially hard to constrain, and climate models neither agree on their phase nor their spatial extent. This is problematic, as models that underestimate contemporary supercooled-liquid in MPCs will underestimate future warming. Furthermore, it has recently been shown that supercooled-liquid water in MPCs is not homogeneously-mixed, neither vertically nor horizontally. However, while there have been attempts at observationally constraining MPCs to constrain uncertainties in future warming, all studies only use the phase of the interior of MPCs. Using novel satellite observations, and contrary to current knowledge, we show that MPCs are more liquid at the cloud top globally. We use these observations to constrain, for the first time, the cloud top phase in addition to the interior of MPCs in a global climate model, leading to +1C more 21st century warming in NorESM2 SSP5-8.5 climate projections. We anticipate that the difference between cloud top and interior phase in MPCs is an important new target metric for future climate model development, because similar MPC-related biases in future warming are likely present in many climate models.
Association of intraoperative cerebral and somatic tissue oxygen saturation with postoperative acute kidney injury in adult patients undergoing multiple valve surgery
Authors: Zhang Hui , Zhang Taoyuan , Hou Lihong , Zhao Jing , Fan Qianqian , Wang Lini , Lu Zhihong , Dong Hailong , Lei Chong 
Published: June 2023

Abstract
BACKGROUND
The association between tissue oxygenation with postoperative acute kidney injury (AKI) in adult patients undergoing multiple valve surgery has not been specifically studied.
METHODS
In this prospective exploratory cohort study, 99 patients were enrolled. The left forehead, the left forearm, the left upper thigh, and the left renal region tissue oxygen saturation using near-infrared spectroscopy were monitored. The association between each threshold and AKI was assessed. The relative and absolute thresholds were < 70%, < 75%, < 80%, < 85%, < 90%, < 95%, and < 100% baseline, and baseline-standard deviation (SD), -1.5 SD, -2 SD, -2.5 SD, and − 3 SD. Multivariate logistic regression analysis was adopted to explore the association.
RESULTS
AKI occurred in 53 (54%) patients. The absolute value-based SrrO2 thresholds associated with AKI were baseline-3 SD (odds ratio [OR], 4.629; 95% confidence interval [CI], 1.238–17.314; P = 0.023) and baseline-2.5 SD (OR, 2.842; 95% CI, 1.025–7.881; P = 0.045) after adjusting for the potential confounders, those are renal region tissue oxygen saturation of 55% and 60%, but not statistically significant after correcting for multiple testing (corrected P = 0.114 and 0.179, respectively).
CONCLUSION
The SrrO2 desaturation, defined as < baseline – 2.5 SD or < baseline – 3 SD, may be associated with AKI. The thresholds need to be verified in future large-scale studies.
The study was registered at ClinicalTrials.gov, first trial registration: 26/10/2017, identifier: NCT03323203.
Vehicle Crash Simulation Models for Reinforcement Learning driven crash-detection algorithm calibration
Authors: Afraj Shahabaz , Vaculín Ondřej , Böhmländer Dennis , Hynčík Luděk 
Published: June 2023

Abstract
The development of finite element vehicle models for crash simulations is a highly complex task. The main aim of these models is to simulate a variety of crash scenarios and assess all the safety systems for their respective performances. These vehicle models possess a substantial amount of data pertaining to the vehicle's geometry, structure, materials, etc., and are used to estimate a large set of system and component level characteristics using crash simulations. It is understood that even the most well-developed simulation models are prone to deviations in estimation when compared to real-world physical test results. This is generally due to our inability to model the chaos and uncertainties introduced in the real world. Such unavoidable deviations render the use of virtual simulations ineffective for the calibration process of the algorithms that activate the restraint systems in the event of a crash (crash-detection algorithm). In the scope of this research, authors hypothesize the possibility of accounting for such variations introduced in the real world by creating a feedback loop between real-world crash tests and crash simulations. To accomplish this, a Reinforcement Learning (RL) compatible virtual surrogate model is used, which is adapted from crash simulation models. Hence, a conceptual methodology is illustrated in this paper for developing an RL-compatible model that can be trained using the results of crash simulations and crash tests. As the calibration of the crash-detection algorithm is fundamentally dependent upon the crash pulses, the scope of the expected output is limited to advancing the ability to estimate crash pulses. Furthermore, the real-time implementation of the methodology is illustrated using an actual vehicle model.
Factors associated with unfavorable treatment outcomes among multidrug-resistant tuberculosis patients, Sierra Leone: A cross-sectional secondary data analysis
Authors: Koroma Josephine Amie , Elduma Adel Hussein , Sessay Umaru , Gebru Gebrekrstos Negash 
Published: June 2023

Abstract

Background
Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally. The tuberculosis rate in Sierra Leone is 298 per 100,000 people, and it is considered a country with a high burden of tuberculosis. In Sierra Leone, there is limited information on the outcomes of the treatment of MDR-TB, especially those exacerbated by COVID-19. We identified factors associated with unfavorable treatment outcomes among patients with MDR-TB in Sierra Leone.
Methods
We conducted a cross-sectional study to analyze hospital-based MDR-TB data for 2017 to 2021. Demographic, clinical, and treatment outcome data were extracted from the main MDR-TB referral hospital database. We defined unfavorable outcomes as patients who died were lost to follow-up, or defaulted. We calculate adjusted odds ratios (aOR) and 95% confidence intervals (CI) to identify predictors of the outcomes of MDR-TB treatment.
Results
Between 2017 and 2021, 628 patients with MDR-TB were reported in the Lakka hospital; 441 (71%) were male, with a median age of 34 years (range: 1–70). Clinically, 21% of the 628 MDR-TB patients were HIV positive and 413 were malnourished (66%). Seventy percent (440) of MDR-TB patients received tuberculosis treatment. The majority of patients, 457 (73%), were treated with the short treatment regimen, and 126 (20%) experienced unfavorable outcomes. Age group less than 20 years (aOR = 5.08; CI:1.87–13.82), tuberculosis retreatment (aOR = 3.23; CI:1.82–5.73), age group 21–45 years (aOR = 2.22; CI:140–3.54), HIV (aOR = 2.16; CI:1.33–3.53), malnourishment (aOR = 1.79; CI:1.12–2.86) are significantly associated with unfavorable treatment outcome for DR-TB.
Conclusions
This analysis found a high proportion of unfavorable treatment outcomes among MDR-TB patients in Sierra Leone. Malnourishment, TB retreatment, HIV co-infection, and people aged 45 years were associated with unfavorable outcomes of MDR-TB treatment. Increasing patients’ awareness, mainly among the youngest, heightens treatment adherence and HIV monitoring can reduce adverse treatment outcomes in Sierra Leone and other Sub-Saharan African countries.
The Effect of Intradialytic Exercise on Calcium, Phosphorus and Parathyroid Hormone: A Randomized Controlled Trial
Authors: Tabibi Mohammad Ali , Wilund Kenneth R , Salimian Nasrin , Nikbakht Saghar , Soleymany Mahsa , Roshanaeian Zahra , Nazemi Farzad , Ahmadi Saghar 
Published: June 2023

Abstract
Background
Patients with kidney failure experience derangements of circulating markers of mineral metabolism and dysregulation of skeletal and cardiovascular physiology which results in high mortality rate in these patients. This study aimed to evaluate the effect of intradialytic exercise on regulation of these abnormalities in patients receiving chronic hemodialysis (HD).
Methods
In this randomized controlled trial conducted in an HD center in Iran, adult patients receiving chronic HD were randomized to intradialytic exercise (60 minutes) in the second hour of thrice weekly dialysis for 6 months (intervention) or no intradialytic exercise (control). The primary outcomes were serum calcium, serum phosphorous and parathyroid hormone levels. Secondary outcomes were serum alkaline phosphatase and calcium-phosphorous product
Results
The study included 44 participants randomized to intervention (n = 22) or control (n = 22). During the 6-month intervention period, significant between-group changes were observed in all primary and secondary outcomes between the intervention and control groups. Statistical analyses reveal a significant increase in serum calcium (P < 0.05) as well as a significant decrease in serum phosphorous, parathyroid hormone, alkaline phosphatase and calcium-phosphorous product (P < 0.05).
Conclusion
Intradialytic exercise performed for at least 60 minutes during thrice weekly dialysis sessions improves bone mineral metabolism in adult patients receiving HD. Further studies should focus on observing and comparing the effect of different types of exercise on bone mineral disorders and all-cause mortality in HD patients.
Trial registration
ClinicalTrials.gov Identifier: NCT04916743, Registered on 08/06/2021. Registered trial name: The Effect of Intradialytic Exercise on Calcium, Phosphorous and Parathyroid Hormone: A Randomized Controlled Trial
Optimization of hydrolysis of whey protein concentrate using trypsin-like microbial protease and evaluation of dipeptidyl peptidase inhibitory activity of the obtained hydrolyzed product
Authors: Mustafa Muhannad Haj , Soleimanian-Zad Sabihe , Albukhaty Salim 
Published: June 2023

Abstract
Whey proteins are abundant in peptides that possess various biological activities. In order to enhance the biological properties of protein hydrolysates, it is essential to optimize the conditions of the hydrolysis process as much as possible. Firstly, we have determined the optimal conditions for hydrolysis of whey protein concentrate (WPC) by by filtered trypsin-like protease (FTLP) in vitro conditions. Then, the ability of obtained whey protein hydrolysates against inhibit dipeptidyl peptidase-4 (DiPP4) in vitro conditions was examined. The optimum point for WPC hydrolysis by FTLP with the predicted optimal level of Degree of hydrolysis (DH, 42.9 %) was at an E:S ratio of 5:100 (w/w), 8.6 h, and a temperature of 40 °C. The factual DH under ideal conditions was 42.04 %, indicating the efficiency of the selected model (P ≤ 0.05). The findings indicated that hydrolysates of WPC generated by FTLP, including both the unfractionated section and the fractions obtained via ultrafiltration using 10- and 5-kDa cut-off membranes, exhibited anti-diabetic characteristics. However, the fractions exhibited greater inhibitory effects against the DiPP4 enzyme, with IC50 values of 1.98, 1.19, and 0.9 mg/mL for the unfractionated section, 10-kDa fraction, and 5-kDa fraction, respectively. Moreover, the results indicated that probiotic L. plantarum subsp. plantarumPTCC 1896 or its components may provide opportunities for future management of type-Ⅱ diabetes by inhibiting DiPP4.
Pathological roles of bone marrow adipocyte-derived monocyte chemotactic protein-1 in aggravating glucose metabolism disorder in type 2 diabetes
Authors: Wan Shan , Xie Jinwei , Liang Yan , Yu Xijie 
Published: June 2023

Abstract
Objective
To explore the effect and related mechanisms of bone marrow adipocyte (BMA)-derived monocyte chemotactic protein-1 (Mcp-1) on systemic glucose homeostasis in type 2 diabetes (T2D).
Methods
Transcriptome sequence, qPCR, ELISA, and immunofluorescence staining were utilized to investigate the alterations in BMA and pancreatic beta-cells. Meanwhile, cell counting kit, flow cytometry, and ELISA were employed to identify cell viability, apoptosis, and glucose-stimulated insulin secretion (GSIS) levels. The protein expression levels were examined using WB.
Results
Compared with Control (Ctrl) group, the gene expression of whole bone marrow cells from db/db mice showed significant changes, among which Mcp-1 was dramatically up-regulated (Log2FC = 2.88), and its major enriched pathway included chemokine signaling pathway and AGE-RAGE signaling pathway in diabetic complications. The BMA was accumulated, the proliferation and GSIS of beta-cells were decreased in T2D mice. The mRNA expression of Mcp-1 in BMA from T2D mice was notably up-regulated. The levels of Mcp-1 in serum and bone marrow supernatant, and the Ccr2 receptor of beta-cells in T2D mice were signally increased. Additionally, the level of Mcp-1 was distinctively elevated in BMA-derived conditional media (CM), which markedly inhibited the proliferation, GSIS and the protein level of p-Akt of the beta-cells. After blocking Mcp-1 pathway in T2D mice, the protein level of p-Akt and the proliferation of beta-cells were restored, the disordered glucose homeostasis was overtly improved as well.
Conclusion
BMA are accumulated in T2D, which secrete excessive Mcp-1 to aggravate the abnormal accumulation of BMA in bone marrow cavity through paracrine pathway. The up-regulated Mcp-1 can further deteriorate glucose metabolism disorder via restraining the proliferation and insulin secretion of beta-cells by endocrine pathway. Repression of Mcp-1 signaling can partly restore the proliferation and insulin secretion of beta-cells and improve the glucose metabolism disorder.
Different strategies to deal with the same issue: the case study of four Brazilian native grasses exposed to an excess of Zn availability.
Authors: FERREIRA PAULO , Milanesi Gabriela Descovi , Santana Natielo Almeida , Tarouco Camila Peligrinotti , Machado Liliane Correa , Silva Isley Cristiellem Bicalho da , Coronas Mariana Vieira , Brunetto Gustavo , Cesco Stefano , Mimmo Tanja , Nicoloso Fernando Teixeira 
Published: June 2023

Abstract
Introduction
The successive applications of fungicides containing zinc (Zn) in vineyards increase the levels of Zn contents in soils, contributing to the reduction of productivity in vineyards in tropical and subtropical regions. Thus, it is necessary to establish sustainable strategies to decrease the toxicity potential of Zn. The selection of tolerant native plants from the Pampa biome present in vineyards can reduce Zn toxicity in vines.
Objective
The study aimed to evaluate, considering physiological variables, the grass species native to the Pampa biome that are more tolerant to Zn excess.
Methods
The grass species of the Pampa biome used in the experiment were Andropogon lateralis, Axonopus affinis, Paspalum plicatulum and Paspalum notatum. Three Zn doses were added to the solution, corresponding to 2µM (original concentration of the nutrient solution), 150 and 300 µM of Zn in the form of ZnCl2 for the cultivation of the four grasses.
Results
The increase in Zn availability increased the photosynthetic rate in the four species. Andropogon lateralis increased shoot and root dry matter production with increasing Zn concentration in solution. Andropogon lateralis increased phosphorus (P) retention in the root system, contributing to the increase of Zn in the roots.
Conclusions
Axonopus affinis, Paspalum notatum, Paspalum plicatulum and Andropogon lateralis presented different values of growth rate, nutritional and photosynthetic parameters, and enzymatic activity, when cultivated in solution with Zn doses.
Do vermicompost applications improve pharmaceutically important alkaloids, growth performance, phenolic content, and defense enzyme activities in summer snowflake ( Leucojum aestivum L.)?
Authors: Cimen Ayca , Baba Yavuz , Yıldırım Arzu Birinci , Turker Arzu Ucar 
Published: June 2023

Abstract

Leucojum aestivum L. includes galanthamine and lycorine, which are two pharmaceutically valuable alkaloids. Vermicompost (VC), an organic waste product created by earthworms enhances soil quality and can improve the medicinal quality of the plant that is crucial to the pharmaceutical industry. Purpose The aim of this study was to determine the effects of four different VC concentrations (5%, 10%, 25%, and 50%) on L. aestivum growth parameters, alkaloid levels (galanthamine and lycorine), total phenol-flavonoid content, free radical scavenging potential, and defense enzyme activities (SOD and CAT) compared to control (no VC). Methods A 2,2-diphenyl-1-picrylhydrazil (DPPH) radical assay was performed for antioxidant activity. Folin-Ciocaltaeu and aluminum chloride colorimetric methods were applied for the determination of total phenol and flavonoid content, respectively. Alkaloid amounts (galanthamine and lycorine) were analyzed by the HPLC-DAD system. Results The width, length, and fresh weight of the leaves were improved by 10% VC treatment. The highest total phenolic content was found in bulbs and leaves treated with 50% VC. HPLC-DAD analysis of alkaloids showed that 10% and 50% VC treatments contained the most galanthamine in the bulb and leaf extracts, respectively. The application of 25% VC was the most efficient in terms of lycorine content in both extracts. CAT activity was elevated at 10%, 25%, and 50% VC. Conclusions Based on the growth performance and galanthamine content of the bulbs and leaves, it can be concluded that a 10% VC application was the most effective in the cultivation of L. aestivum.