bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing feasible healthy diets for Ethiopian women of reproductive age: a linear goal programming approach
Authors: Bekele Tesfaye Hailu , van Rooijen Maike , Gerdessen Johanna C , Brouwer Inge D , Feskens Edith JM , Trijsburg Laura , Alemayehu Dawit , de Vries Jeanne HM 
Published: July 2023

AbstractObjective:To develop a healthy diet for Ethiopian women closely resembling their current diet and taking fasting periods into account while tracking the cost difference.Design:Linear goal programming models were built for three scenarios (non-fasting, continuous fasting and intermittent fasting). Each model minimised a function of deviations from nutrient reference values for eleven nutrients (protein, Ca, Fe, Zn, folate, and the vitamins A, B1, B2, B3, B6, and B12). The energy intake in optimised diets could only deviate 5 % from the current diet.Settings:Five regions are included in the urban and rural areas of Ethiopia.Participants:Two non-consecutive 24-h dietary recalls (24HDR) were collected from 494 Ethiopian women of reproductive age from November to December 2019.Results:Women’s mean energy intake was well above 2000 kcal across all socio-demographic subgroups. Compared to the current diet, the estimated intake of several food groups was considerably higher in the optimised modelled diets, that is, milk and dairy foods (396 v. 30 g/d), nuts and seeds (20 v. 1 g/d) and fruits (200 v. 7 g/d). Except for Ca and vitamin B12 intake in the continuous fasting diet, the proposed diets provide an adequate intake of the targeted micronutrients. The proposed diets had a maximum cost of 120 Ethiopian birrs ($3·5) per d, twice the current diet’s cost.Conclusion:The modelled diets may be feasible for women of reproductive age as they are close to their current diets and fulfil their energy and nutrient demands. However, the costs may be a barrier to implementation.
Assessment of health-care workers’ attitudes, knowledge, and skills in the care of critically ill Muslim children in New York
Authors: Ijaz Nadir , Hassan Amir , Ghannoum Sarah , Choudhury Tarif , Piracha Natasha 
Published: July 2023

AbstractObjectivesPediatric health-care workers often care for families of minority religious backgrounds, but little is known about their perspective in providing culturally and spiritually appropriate care for Muslim patients. We aimed to (1) characterize the attitudes, knowledge, and skills of health-care workers in the care of critically ill Muslim children and (2) evaluate preferences for different educational interventions to improve care of critically ill Muslim children.MethodsWe administered a single-center, cross-sectional, 33-question, electronic survey of interdisciplinary health-care workers in a large pediatric intensive care unit in New York City to characterize their attitudes, knowledge, and skills in caring for critically ill Muslim children.ResultsOf 413 health-care workers surveyed, there were 109 (26%) respondents. Participants responded correctly to 51.7 ± 22.2% (mean ± SD) and 69.2 ± 20.6% of background knowledge and clinical skills questions, respectively. Only 29.8% of participants perceived adequate institutional resources to provide culturally competent care to Muslim patients and their families. Participants identified end-of-life care (47.5%) and bioethical concerns (45%) as needed areas for additional institutional resources. When asked about support to aid in caring for Muslim patients, 43.4% of participants requested a team of Muslim health-care workers to provide guidance. Participants most often requested video-based training modules (32.5%) and written materials (30%) as potential educational interventions.Significance of resultsWe identify gaps in health-care worker knowledge and skills in the care of the critically ill Muslim child. We also describe possible areas for intervention to facilitate culturally and spiritually appropriate care delivery to Muslim children and families.
Anglican Confirmation: an Unfinished Reform
Authors: Buchanan Colin 
Published: July 2023

AbstractConfirmation, one of the seven sacraments in Lombard’s schedule, was retained by the English Reformers, but not as a sacrament and without any distinctive quasi-sacramental grace attributed to it. It became a ceremony to complete the catechizing process for children who, having been baptized as infants, were at the age of discretion now to come to holy communion. The reformers thought that a post-baptismal laying on of hands had been practised from apostolic times, and so commended the ceremony for their church practice. This requirement enabled later generations, such as Gregory Dix, to bid up its significance, teaching that confirmation completes baptism, and thus that water-baptism is of itself incomplete. The underlying premise has been that from the apostles onwards a requisite second initiatory ceremony invariably followed baptism. Both Bible and early church history contradict this thesis, not least the eight post-apostolic authors of the first two centuries who mention baptism. All eight testify to the use of water without any further ceremony. Thus, any insistence upon a two-stage sacramental initiation today lacks historical foundations; Anglicans ought to review residual texts and practices which reflect such a pattern.
Early neurodevelopment in the offspring of women enrolled in a randomized controlled trial assessing the effectiveness of a nutrition + exercise intervention on the cognitive development of 12-month-olds
Authors: Mortaji Neda , Krzeczkowski John , Atkinson Stephanie , Amani Bahar , Schmidt Louis A. , Van Lieshout Ryan J. 
Published: July 2023

AbstractExperimental data on the effects of lifestyle interventions on fetal neurodevelopment in humans remain scarce. This study assessed the impact of a pregnancy nutrition+exercise intervention on offspring neurodevelopment at 12 months of age. The Be Healthy in Pregnancy (BHIP) randomized controlled trial (RCT) randomly assigned pregnant persons with stratification by site and body mass index (BMI) to bi-weekly nutrition counselling and high dairy protein diet, walking goal of 10,000 steps/day plus usual prenatal care (UPC; intervention group) or UPC alone (control group). This study examined a subset of these mothers (> 18 years, singleton pregnancy, BMI <40 kg/m2, and enrolled by ≤12 weeks gestation) and their infants (intervention = 42, control = 32), assessing cognition, language, motor, social-emotional, and adaptive functioning at 12 months using the Bayley Scales of Infant and Toddler Development third edition (BSID-III) as the outcome measure. We also examined if maternal factors (prepregnancy BMI, gestational weight gain (GWG)) moderated associations. Expressive language (MD = 9.62, 95% CI = (9.05–10.18), p = 0.03, ƞ2p = 0.07) and general adaptive composite (GAC) scores (MD = 103.97, 95% CI = (100.31–107.63), p = 0.04, ƞ2p = 0.06) were higher in infants of mothers in the intervention group. Effect sizes were medium. However, mean cognitive, receptive language, motor, and social-emotional scale scores did not differ between groups. A structured and monitored nutrition+exercise intervention during pregnancy led to improved expressive language and general adaptive behavior in 12-month-olds, but not cognitive, receptive language, motor, or socioemotional functioning. While these experimental data are promising, further research is needed to determine the clinical utility of nutrition+exercise interventions for optimizing infant neurodevelopment.
Substorm Impact on Dayside Ionospheric Currents
Authors: Elhawary R. , Laundal K. M. , Reistad J. P. , Madelaire M. , Ohma A. 
Published: July 2023

AbstractIonospheric dayside dynamics is strongly controlled by the interaction between the Interplanetary Magnetic Field (IMF) and the Earth's magnetic field near the dayside magnetopause, while nightside ionospheric dynamics depends mainly on magnetotail activity. However, we know little about the influence of magnetotail activity on the dayside ionospheric dynamics. We investigate this by performing superposed epoch analyses of ground magnetic field data for substorms occurring during northward IMF. In such substorms, dayside reconnection is minimized, allowing us to separate the effects of the magnetotail activity on the dayside current system. We find that as nightside activity elevates, the dayside ionospheric current elevates. Our analyses indicate that the lobe cells are less distinct before onset than during non‐substorm northward IMF conditions. They become more pronounced after onset, possibly due to magnetospheric reconfiguration or a remote effect of the nightside current. We discuss possible mechanisms that may explain our observations.
The East‐West Asymmetry of Particle Intensity in Energetic Storm Particle Events
Authors: Ding Zheyi , Li Gang , Santa Fe Dueñas Adolfo , Ebert Robert W. , Wijsen Nicolas , Poedts Stefaan 
Published: July 2023

AbstractWe examine the East‐West asymmetry of the peak intensity in energetic storm particle (ESP) events using the improved Particle Acceleration and Transport in the Heliosphere model. We find that injection efficiency peaks east of the nose of coronal mass ejection shock where the shock exhibits a quasi‐parallel geometry. We show that the peak intensity at the eastern flank is generally larger than that at the western flank and it positively correlates with the injection efficiency. We also examine this asymmetry for heavy ions, which depends sensitively on the ion energy. Comparison between the modeling results with the measurements of ESP events at 1 au shows a reasonable agreement. We suggest that the injection efficiency can be a primary factor leading to the East‐West asymmetry of the peak intensity in ESP events. Additionally, the charge‐to‐mass (Q/A) dependence of the maximum particle energy affects this asymmetry for heavy ions.
Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
Authors: Salz Lucia , Seitz Alexander , Schäfer Daniel , Franzen Julia , Holzer Tatjana , Garcia-Prieto Carlos A. , Bürger Iris , Hardt Olaf , Esteller Manel , Wagner Wolfgang 
Published: July 2023

AbstractChimeric antigen receptor (CAR) T cells provide new perspectives for treatment of hematological malignancies. Manufacturing of these cellular products includes culture expansion procedures, which may affect cellular integrity and therapeutic outcome. In this study, we investigated culture-associated epigenetic changes in CAR T cells and found continuous gain of DNAm, particularly within genes that are relevant for T cell function. Hypermethylation in many genes, such as TCF7, RUNX1, and TOX, was reflected by transcriptional downregulation. 332 CG dinucleotides (CpGs) showed an almost linear gain in methylation with cell culture time, albeit neighboring CpGs were not coherently regulated on the same DNA strands. An epigenetic signature based on 14 of these culture-associated CpGs predicted cell culture time across various culture conditions. Notably, even in CAR T cell products of similar culture time higher DNAm levels at these CpGs were associated with significantly reduced long-term survival post transfusion. Our data demonstrate that cell culture expansion of CAR T cells evokes DNA hypermethylation at specific sites in the genome and the signature may also reflect loss of potential in CAR T cell products. Hence, reduced cultivation periods are beneficial to avoid dysfunctional methylation programs that seem to be associated with worse therapeutic outcome.
Mcr-dependent methanogenesis in Archaeoglobaceae enriched from a terrestrial hot spring
Authors: Buessecker Steffen , Chadwick Grayson L , Quan Melanie E , Hedlund Brian P , Dodsworth Jeremy A , Dekas Anne E 
Published: July 2023

Abstract
The preeminent source of biological methane on Earth is methyl coenzyme M reductase (Mcr)-dependent archaeal methanogenesis. A growing body of evidence suggests a diversity of archaea possess Mcr, although experimental validation of hypothesized methane metabolisms has been missing. Here, we provide evidence of a functional Mcr-based methanogenesis pathway in a novel member of the family Archaeoglobaceae, designated Methanoglobus nevadensis, which we enriched from a terrestrial hot spring on the polysaccharide xyloglucan. Our incubation assays demonstrate methane production that is highly sensitive to the Mcr inhibitor bromoethanesulfonate, stimulated by xyloglucan and xyloglucan-derived sugars, concomitant with the consumption of molecular hydrogen, and causing a deuterium fractionation in methane characteristic of hydrogenotrophic and methylotrophic methanogens. Combined with the recovery and analysis of a high-quality M. nevadensis metagenome-assembled genome encoding a divergent Mcr and diverse potential electron and carbon transfer pathways, our observations suggest methanogenesis in M. nevadensis occurs via Mcr and is fueled by the consumption of cross-fed byproducts of xyloglucan fermentation mediated by other community members. Phylogenetic analysis shows close affiliation of the M. nevadensis Mcr with those from Korarchaeota, Nezhaarchaeota, Verstraetearchaeota, and other Archaeoglobales that are divergent from well-characterized Mcr. We propose these archaea likely also use functional Mcr complexes to generate methane on the basis of our experimental validation in M. nevadensis. Thus, divergent Mcr-encoding archaea may be underestimated sources of biological methane in terrestrial and marine hydrothermal environments.
FOXM1 is critical for the fitness recovery of chromosomally unstable cells
Authors: Pan Fan , Chocarro Sara , Ramos Maria , Chen Yuanyuan , Alonso de la Vega Alicia , Somogyi Kalman , Sotillo Rocio 
Published: July 2023

Abstract
Tumor progression and evolution are frequently associated with chromosomal instability (CIN). Tumor cells often express high levels of the mitotic checkpoint protein MAD2, leading to mitotic arrest and cell death. However, some tumor cells are capable of exiting mitosis and consequently increasing CIN. How cells escape the mitotic arrest induced by MAD2 and proliferate with CIN is not well understood. Here, we explored loss-of-function screens and drug sensitivity tests associated with MAD2 levels in aneuploid cells and identified that aneuploid cells with high MAD2 levels are more sensitive to FOXM1 depletion. Inhibition of FOXM1 promotes MAD2-mediated mitotic arrest and exacerbates CIN. Conversely, elevating FOXM1 expression in MAD2-overexpressing human cell lines reverts prolonged mitosis and rescues mitotic errors, cell death and proliferative disadvantages. Mechanistically, we found that FOXM1 facilitates mitotic exit by inhibiting the spindle assembly checkpoint (SAC) and the expression of Cyclin B. Notably, we observed that FOXM1 is upregulated upon aneuploid induction in cells with dysfunctional SAC and error-prone mitosis, and these cells are sensitive to FOXM1 knockdown, indicating a novel vulnerability of aneuploid cells.
Unveiling phase diagram of the lightly doped high-Tc cuprate superconductors with disorder removed
Authors: Kurokawa Kifu , Isono Shunsuke , Kohama Yoshimitsu , Kunisada So , Sakai Shiro , Sekine Ryotaro , Okubo Makoto , Watson Matthew D. , Kim Timur K. , Cacho Cephise , Shin Shik , Tohyama Takami , Tokiwa Kazuyasu , Kondo Takeshi 
Published: July 2023

AbstractThe currently established electronic phase diagram of cuprates is based on a study of single- and double-layered compounds. These CuO2 planes, however, are directly contacted with dopant layers, thus inevitably disordered with an inhomogeneous electronic state. Here, we solve this issue by investigating a 6-layered Ba2Ca5Cu6O12(F,O)2 with inner CuO2 layers, which are clean with the extremely low disorder, by angle-resolved photoemission spectroscopy (ARPES) and quantum oscillation measurements. We find a tiny Fermi pocket with a doping level less than 1% to exhibit well-defined quasiparticle peaks which surprisingly lack the polaronic feature. This provides the first evidence that the slightest amount of carriers is enough to turn a Mott insulating state into a metallic state with long-lived quasiparticles. By tuning hole carriers, we also find an unexpected phase transition from the superconducting to metallic states at 4%. Our results are distinct from the nodal liquid state with polaronic features proposed as an anomaly of the heavily underdoped cuprates.
Breathing porous liquids based on responsive metal-organic framework particles
Authors: Koutsianos Athanasios , Pallach Roman , Frentzel-Beyme Louis , Das Chinmoy , Paulus Michael , Sternemann Christian , Henke Sebastian 
Published: July 2023

AbstractResponsive metal-organic frameworks (MOFs) that display sigmoidal gas sorption isotherms triggered by discrete gas pressure-induced structural transformations are highly promising materials for energy related applications. However, their lack of transportability via continuous flow hinders their application in systems and designs that rely on liquid agents. We herein present examples of responsive liquid systems which exhibit a breathing behaviour and show step-shaped gas sorption isotherms, akin to the distinct oxygen saturation curve of haemoglobin in blood. Dispersions of flexible MOF nanocrystals in a size-excluded silicone oil form stable porous liquids exhibiting gated uptake for CO2, propane and propylene, as characterized by sigmoidal gas sorption isotherms with distinct transition steps. In situ X-ray diffraction studies show that the sigmoidal gas sorption curve is caused by a narrow pore to large pore phase transformation of the flexible MOF nanocrystals, which respond to gas pressure despite being dispersed in silicone oil. Given the established flexible nature and tunability of a range of MOFs, these results herald the advent of breathing porous liquids whose sorption properties can be tuned rationally for a variety of technological applications.
Structural delineation and computational design of SARS-CoV-2-neutralizing antibodies against Omicron subvariants
Authors: Moriyama Saya , Anraku Yuki , Taminishi Shunta , Adachi Yu , Kuroda Daisuke , Kita Shunsuke , Higuchi Yusuke , Kirita Yuhei , Kotaki Ryutaro , Tonouchi Keisuke , Yumoto Kohei , Suzuki Tateki , Someya Taiyou , Fukuhara Hideo , Kuroda Yudai , Yamamoto Tsukasa , Onodera Taishi , Fukushi Shuetsu , Maeda Ken , Nakamura-Uchiyama Fukumi , Hashiguchi Takao , Hoshino Atsushi , Maenaka Katsumi , Takahashi Yoshimasa 
Published: July 2023

AbstractSARS-CoV-2 Omicron subvariants have evolved to evade receptor-binding site (RBS) antibodies that exist in diverse individuals as public antibody clones. We rationally selected RBS antibodies resilient to mutations in emerging Omicron subvariants. Y489 was identified as a site of virus vulnerability and a common footprint of broadly neutralizing antibodies against the subvariants. Multiple Y489-binding antibodies were encoded by public clonotypes and additionally recognized F486, potentially accounting for the emergence of Omicron subvariants harboring the F486V mutation. However, a subclass of antibodies broadly neutralized BA.4/BA.5 variants via hydrophobic binding sites of rare clonotypes along with high mutation-resilience under escape mutation screening. A computationally designed antibody based on one of the Y489-binding antibodies, NIV-10/FD03, was able to bind XBB with any 486 mutation and neutralized XBB.1.5. The structural basis for the mutation-resilience of this Y489-binding antibody group may provide important insights into the design of therapeutics resistant to viral escape.
Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration
Authors: Pang Xiaojie , Nawrocki Wojciech J. , Cardol Pierre , Zheng Mengyuan , Jiang Jingjing , Fang Yuan , Yang Wenqiang , Croce Roberta , Tian Lijin 
Published: July 2023

AbstractWhile photosynthesis transforms sunlight energy into sugar, aerobic and anaerobic respiration (fermentation) catabolizes sugars to fuel cellular activities. These processes take place within one cell across several compartments, however it remains largely unexplored how they interact with one another. Here we report that the weak acids produced during fermentation down-regulate both photosynthesis and aerobic respiration. This effect is mechanistically explained with an “ion trapping” model, in which the lipid bilayer selectively traps protons that effectively acidify subcellular compartments with smaller buffer capacities – such as the thylakoid lumen. Physiologically, we propose that under certain conditions, e.g., dim light at dawn, tuning down the photosynthetic light reaction could mitigate the pressure on its electron transport chains, while suppression of respiration could accelerate the net oxygen evolution, thus speeding up the recovery from hypoxia. Since we show that this effect is conserved across photosynthetic phyla, these results indicate that fermentation metabolites exert widespread feedback control over photosynthesis and aerobic respiration. This likely allows algae to better cope with changing environmental conditions.
Universal alignment in turbulent pair dispersion
Authors: Shnapp Ron , Brizzolara Stefano , Neamtu-Halic Marius M. , Gambino Alessandro , Holzner Markus 
Published: July 2023

AbstractCountless processes in nature and industry, from rain droplet nucleation to plankton interaction in the ocean, are intimately related to turbulent fluctuations of local concentrations of advected matter. These fluctuations can be described by considering the change of the separation between particle pairs, known as pair dispersion, which is believed to obey a cubic in time growth according to Richardson’s theory. Our work reveals a universal, scale-invariant alignment between the relative velocity and position vectors of dispersing particles at a mean angle that we show to be a universal constant of turbulence. We connect the value of this mean angle to Richardson’s traditional theory and find agreement with data from a numerical simulation and a laboratory experiment. While the Richardson’s cubic regime has been observed for small initial particle separations only, the constancy of the mean angle manifests throughout the entire inertial range of turbulence. Thus, our work reveals the universal nature of turbulent pair dispersion through a geometrical paradigm whose validity goes beyond the classical theory, and provides a framework for understanding and modeling transport and mixing processes.
Increased body mass index is linked to systemic inflammation through altered chromatin co-accessibility in human preadipocytes
Authors: Garske Kristina M. , Kar Asha , Comenho Caroline , Balliu Brunilda , Pan David Z. , Bhagat Yash V. , Rosenberg Gregory , Koka Amogha , Das Sankha Subhra , Miao Zong , Sinsheimer Janet S. , Kaprio Jaakko , Pietiläinen Kirsi H. , Pajukanta Päivi 
Published: July 2023

AbstractObesity-induced adipose tissue dysfunction can cause low-grade inflammation and downstream obesity comorbidities. Although preadipocytes may contribute to this pro-inflammatory environment, the underlying mechanisms are unclear. We used human primary preadipocytes from body mass index (BMI) -discordant monozygotic (MZ) twin pairs to generate epigenetic (ATAC-sequence) and transcriptomic (RNA-sequence) data for testing whether increased BMI alters the subnuclear compartmentalization of open chromatin in the twins’ preadipocytes, causing downstream inflammation. Here we show that the co-accessibility of open chromatin, i.e. compartmentalization of chromatin activity, is altered in the higher vs lower BMI MZ siblings for a large subset ( ~ 88.5 Mb) of the active subnuclear compartments. Using the UK Biobank we show that variants within these regions contribute to systemic inflammation through interactions with BMI on C-reactive protein. In summary, open chromatin co-accessibility in human preadipocytes is disrupted among the higher BMI siblings, suggesting a mechanism how obesity may lead to inflammation via gene-environment interactions.