bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between lifestyle habits and glaucoma incidence: a retrospective cohort study
Authors: Fujita Asahi , Hashimoto Yohei , Matsui Hiroki , Yasunaga Hideo , Aihara Makoto 
Published: April 2023

Abstract
Background/Objectives
Although lifestyle habits may represent modifiable risk factors of glaucoma, the association between lifestyle factors and glaucoma is not well understood. The aim of this study was to investigate the association between lifestyle habits and the development of glaucoma.

Subjects/Methods
Participants who underwent health check-ups from 2005 to 2020 using a large-scale administrative claims database in Japan were included in the study. Cox regression analyses were performed where glaucoma development was regressed on the lifestyle (body mass index, current smoking, frequency and amount of alcohol consumption, eating habits, exercise habits and quality of sleep), age, sex, hypertension, diabetes mellitus and dyslipidaemia.

Results
Among the 3,110,743 eligible individuals, 39,975 developed glaucoma during the mean follow-up of 2058 days. Factors associated with increased risk of glaucoma were overweight/obese (vs. moderate weight: hazard ratio, 1.04 [95% confidence interval, 1.02–1.07]), alcohol consumption of 2.5–4.9 units/day, 5–7.4 units/day, and ≥7.5 units/day (vs. <2.5 units/day: 1.05 [1.02–1.08], 1.05 [1.01–1.08] and 1.06 [1.01–1.12], respectively), skipping breakfast (1.14 [1.10–1.17]), late dinner (1.05 [1.03-1.08]) and daily walking of 1 h (1.14 [1.11–1.16]). Factors associated with decreased risk of glaucoma were daily alcohol consumption (vs. rarely: 0.94 [0.91–0.97]) and regular exercise (0.92 [0.90–0.95]).

Conclusions
Moderate body mass index, having breakfast, avoiding late dinner, limiting alcohol intake to <2.5 units/day, and regular exercise were associated with a reduced risk of developing glaucoma in the Japanese population. These findings may be useful for promoting glaucoma prophylaxis.
Observation of phonon Poiseuille flow in isotopically purified graphite ribbons
Authors: Huang Xin , Guo Yangyu , Wu Yunhui , Masubuchi Satoru , Watanabe Kenji , Taniguchi Takashi , Zhang Zhongwei , Volz Sebastian , Machida Tomoki , Nomura Masahiro 
Published: April 2023

AbstractIn recent times, the unique collective transport physics of phonon hydrodynamics motivates theoreticians and experimentalists to explore it in micro- and nanoscale and at elevated temperatures. Graphitic materials have been predicted to facilitate hydrodynamic heat transport with their intrinsically strong normal scattering. However, owing to the experimental difficulties and vague theoretical understanding, the observation of phonon Poiseuille flow in graphitic systems remains challenging. In this study, based on a microscale experimental platform and the pertinent occurrence criterion in anisotropic solids, we demonstrate the existence of the phonon Poiseuille flow in a 5.5 μm-wide, suspended and isotopically purified graphite ribbon up to a temperature of 90 K. Our observation is well supported by our theoretical model based on a kinetic theory with fully first-principles inputs. Thus, this study paves the way for deeper insight into phonon hydrodynamics and cutting-edge heat manipulating applications.
Disrupting the α-synuclein-ESCRT interaction with a peptide inhibitor mitigates neurodegeneration in preclinical models of Parkinson’s disease
Authors: Nim Satra , O’Hara Darren M. , Corbi-Verge Carles , Perez-Riba Albert , Fujisawa Kazuko , Kapadia Minesh , Chau Hien , Albanese Federica , Pawar Grishma , De Snoo Mitchell L. , Ngana Sophie G. , Kim Jisun , El-Agnaf Omar M. A. , Rennella Enrico , Kay Lewis E. , Kalia Suneil K. , Kalia Lorraine V. , Kim Philip M. 
Published: April 2023

AbstractAccumulation of α-synuclein into toxic oligomers or fibrils is implicated in dopaminergic neurodegeneration in Parkinson’s disease. Here we performed a high-throughput, proteome-wide peptide screen to identify protein-protein interaction inhibitors that reduce α-synuclein oligomer levels and their associated cytotoxicity. We find that the most potent peptide inhibitor disrupts the direct interaction between the C-terminal region of α-synuclein and CHarged Multivesicular body Protein 2B (CHMP2B), a component of the Endosomal Sorting Complex Required for Transport-III (ESCRT-III). We show that α-synuclein impedes endolysosomal activity via this interaction, thereby inhibiting its own degradation. Conversely, the peptide inhibitor restores endolysosomal function and thereby decreases α-synuclein levels in multiple models, including female and male human cells harboring disease-causing α-synuclein mutations. Furthermore, the peptide inhibitor protects dopaminergic neurons from α-synuclein-mediated degeneration in hermaphroditic C. elegans and preclinical Parkinson’s disease models using female rats. Thus, the α-synuclein-CHMP2B interaction is a potential therapeutic target for neurodegenerative disorders.
A VEL3 histone deacetylase complex establishes a maternal epigenetic state controlling progeny seed dormancy
Authors: Chen Xiaochao , MacGregor Dana R. , Stefanato Francesca L. , Zhang Naichao , Barros-Galvão Thiago , Penfield Steven 
Published: April 2023

AbstractMother plants play an important role in the control of dormancy and dispersal characters of their progeny. In Arabidopsis seed dormancy is imposed by the embryo-surrounding tissues of the endosperm and seed coat. Here we show that VERNALIZATION5/VIN3-LIKE 3 (VEL3) maintains maternal control over progeny seed dormancy by establishing an epigenetic state in the central cell that primes the depth of primary seed dormancy later established during seed maturation. VEL3 colocalises with MSI1 in the nucleolus and associates with a histone deacetylase complex. Furthermore, VEL3 preferentially associates with pericentromeric chromatin and is required for deacetylation and H3K27me3 deposition established in the central cell. The epigenetic state established by maternal VEL3 is retained in mature seeds, and controls seed dormancy in part through repression of programmed cell death-associated gene ORE1. Our data demonstrates a mechanism by which maternal control of progeny seed physiology persists post-shedding, maintaining parental control of seed behaviour.
Identification of d-arabinan-degrading enzymes in mycobacteria
Authors: Al-Jourani Omar , Benedict Samuel T. , Ross Jennifer , Layton Abigail J. , van der Peet Phillip , Marando Victoria M. , Bailey Nicholas P. , Heunis Tiaan , Manion Joseph , Mensitieri Francesca , Franklin Aaron , Abellon-Ruiz Javier , Oram Sophia L. , Parsons Lauren , Cartmell Alan , Wright Gareth S. A. , Baslé Arnaud , Trost Matthias , Henrissat Bernard , Munoz-Munoz Jose , Hirt Robert P. , Kiessling Laura L. , Lovering Andrew L. , Williams Spencer J. , Lowe Elisabeth C. , Moynihan Patrick J. 
Published: April 2023

AbstractBacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, we identify enzymes that cleave the d-arabinan core of arabinogalactan, an unusual component of the cell wall of Mycobacterium tuberculosis and other mycobacteria. We screened 14 human gut-derived Bacteroidetes for arabinogalactan-degrading activities and identified four families of glycoside hydrolases with activity against the d-arabinan or d-galactan components of arabinogalactan. Using one of these isolates with exo-d-galactofuranosidase activity, we generated enriched d-arabinan and used it to identify a strain of Dysgonomonas gadei as a d-arabinan degrader. This enabled the discovery of endo- and exo-acting enzymes that cleave d-arabinan, including members of the DUF2961 family (GH172) and a family of glycoside hydrolases (DUF4185/GH183) that display endo-d-arabinofuranase activity and are conserved in mycobacteria and other microbes. Mycobacterial genomes encode two conserved endo-d-arabinanases with different preferences for the d-arabinan-containing cell wall components arabinogalactan and lipoarabinomannan, suggesting they are important for cell wall modification and/or degradation. The discovery of these enzymes will support future studies into the structure and function of the mycobacterial cell wall.
Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages
Authors: Chernyshev Mark , Sakharkar Mrunal , Connor Ruth I. , Dugan Haley L. , Sheward Daniel J. , Rappazzo C. G. , Stålmarck Aron , Forsell Mattias N. E. , Wright Peter F. , Corcoran Martin , Murrell Ben , Walker Laura M. , Karlsson Hedestam Gunilla B. 
Published: April 2023

AbstractVaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolate 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with the index variant of SARS-CoV-2 and later boosted with mRNA-1273. We characterize mAb genetic features by sequence assignments to the donors’ personal immunoglobulin genotypes and assess antibody neutralizing activities against index SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells are efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.
Temperature sensitive liposome based cancer nanomedicine enables tumour lymph node immune microenvironment remodelling
Authors: Fu Shunli , Chang Lili , Liu Shujun , Gao Tong , Sang Xiao , Zhang Zipeng , Mu Weiwei , Liu Xiaoqing , Liang Shuang , Yang Han , Yang Huizhen , Ma Qingping , Liu Yongjun , Zhang Na 
Published: April 2023

AbstractTargeting tumour immunosuppressive microenvironment is a crucial strategy in immunotherapy. However, the critical role of the tumour lymph node (LN) immune microenvironment (TLIME) in the tumour immune homoeostasis is often ignored. Here, we present a nanoinducer, NIL-IM-Lip, that remodels the suppressed TLIME via simultaneously mobilizing T and NK cells. The temperature-sensitive NIL-IM-Lip is firstly delivered to tumours, then directed to the LNs following pH-sensitive shedding of NGR motif and MMP2-responsive release of IL-15. IR780 and 1-MT induces immunogenic cell death and suppress regulatory T cells simultaneously during photo-thermal stimulation. We demonstrate that combining NIL-IM-Lip with anti-PD-1 significantly enhances the effectiveness of T and NK cells, leading to greatly suppressed tumour growth in both hot and cold tumour models, with complete response in some instances. Our work thus highlights the critical role of TLIME in immunotherapy and provides proof of principle to combine LN targeting with immune checkpoint blockade in cancer immunotherapy.
Utilizing cell-free DNA to predict risk of developing brain metastases in patients with metastatic breast cancer
Authors: Vidula Neelima , Niemierko Andrzej , Hesler Katherine , Ryan Lianne , Moy Beverly , Isakoff Steven , Ellisen Leif , Juric Dejan , Bardia Aditya 
Published: April 2023

AbstractWe compared cell-free DNA (cfDNA) results at MBC diagnosis in patients who developed brain metastases (BM) vs those without (non-BM) to understand genomic predictors of BM. Patients with cfDNA testing at MBC diagnosis (Guardant360®, 73 gene next generation sequencing) were identified. Clinical and genomic features of BM and non-BM were compared (Pearson’s/Wilcoxon rank sum tests). Eighteen of 86 patients (21%) with cfDNA at MBC diagnosis developed BM. Comparing BM vs non-BM, a higher prevalence of BRCA2 (22% vs 4.4%, p = 0.01), APC (11% vs 0%, p = 0.005), CDKN2A (11% vs 1.5%, p = 0.05), and SMAD4 (11% vs 1.5%, p = 0.05) was observed. Seven of 18 BM had ≥1 of the following 4 mutations in baseline cfDNA: APC, BRCA2, CDKN2A or SMAD4 vs 5/68 non-BM (p = 0.001). Absence of this genomic pattern had a high negative predictive value (85%) and specificity (93%) in excluding BM development. Baseline genomic profile varies in MBC that develops BM.
Degradation analysis of tribologically loaded carbon nanotubes and carbon onions
Authors: MacLucas T. , Grützmacher P. , Husmann S. , Schmauch J. , Keskin S. , Suarez S. , Presser V. , Gachot C. , Mücklich F. 
Published: April 2023

AbstractCoating laser-patterned stainless-steel surfaces with carbon nanotubes (CNT) or carbon onions (CO) forms a tribological system that provides effective solid lubrication. Lubricant retention represents the fundamental mechanism of this system, as storing the particles inside the pattern prevents lubricant depletion in the contact area. In previous works, we used direct laser interference patterning to create line patterns with three different structural depths on AISI 304 stainless-steel platelets. Electrophoretic deposition subsequently coated the patterned surfaces with either CNTs or COs. Ball-on-disc friction tests were conducted to study the effect of structural depth on the solid lubricity of as-described surfaces. The results demonstrated that the shallower the textures, the lower the coefficient of friction, regardless of the applied particle type. This follow-up study examines the carbon nanoparticles’ structural degradation after friction testing on substrates patterned with different structural depths (0.24, 0.36, and 0.77 µm). Raman characterization shows severe degradation of both particle types and is used to classify their degradation state within Ferrari’s three-stage amorphization model. It was further shown that improving CNT lubricity translates into increasing particle defectivity. This is confirmed by electron microscopy, which shows decreasing crystalline domains. Compared to CNTs, CO-derived tribofilms show even more substantial structural degradation.
A 3D printable alloy designed for extreme environments
Authors: Smith Timothy M. , Kantzos Christopher A. , Zarkevich Nikolai A. , Harder Bryan J. , Heczko Milan , Gradl Paul R. , Thompson Aaron C. , Mills Michael J. , Gabb Timothy P. , Lawson John W. 
Published: April 2023

AbstractMultiprincipal-element alloys are an enabling class of materials owing to their impressive mechanical and oxidation-resistant properties, especially in extreme environments1,2. Here we develop a new oxide-dispersion-strengthened NiCoCr-based alloy using a model-driven alloy design approach and laser-based additive manufacturing. This oxide-dispersion-strengthened alloy, called GRX-810, uses laser powder bed fusion to disperse nanoscale Y2O3 particles throughout the microstructure without the use of resource-intensive processing steps such as mechanical or in situ alloying3,4. We show the successful incorporation and dispersion of nanoscale oxides throughout the GRX-810 build volume via high-resolution characterization of its microstructure. The mechanical results of GRX-810 show a twofold improvement in strength, over 1,000-fold better creep performance and twofold improvement in oxidation resistance compared with the traditional polycrystalline wrought Ni-based alloys used extensively in additive manufacturing at 1,093 °C5,6. The success of this alloy highlights how model-driven alloy designs can provide superior compositions using far fewer resources compared with the ‘trial-and-error’ methods of the past. These results showcase how future alloy development that leverages dispersion strengthening combined with additive manufacturing processing can accelerate the discovery of revolutionary materials.
Interactions between the lipidome and genetic and environmental factors in autism
Authors: Yap Chloe X. , Henders Anjali K. , Alvares Gail A. , Giles Corey , Huynh Kevin , Nguyen Anh , Wallace Leanne , McLaren Tiana , Yang Yuanhao , Hernandez Leanna M. , Gandal Michael J. , Hansell Narelle K. , Cleary Dominique , Grove Rachel , Hafekost Claire , Harun Alexis , Holdsworth Helen , Jellett Rachel , Khan Feroza , Lawson Lauren P. , Leslie Jodie , Levis Frenk Mira , Masi Anne , Mathew Nisha E. , Muniandy Melanie , Nothard Michaela , Miller Jessica L. , Nunn Lorelle , Strike Lachlan T. , Cadby Gemma , Moses Eric K. ,   The Busselton Health Study Investigators, Hung Joseph , Hui Jennie , Beilby John , de Zubicaray Greig I. , Thompson Paul M. , McMahon Katie L. , Wright Margaret J. , Visscher Peter M. , Dawson Paul A. , Dissanayake Cheryl , Eapen Valsamma , Heussler Helen S. , Whitehouse Andrew J. O. , Meikle Peter J. , Wray Naomi R. , Gratten Jacob 
Published: April 2023

AbstractAutism omics research has historically been reductionist and diagnosis centric, with little attention paid to common co-occurring conditions (for example, sleep and feeding disorders) and the complex interplay between molecular profiles and neurodevelopment, genetics, environmental factors and health. Here we explored the plasma lipidome (783 lipid species) in 765 children (485 diagnosed with autism spectrum disorder (ASD)) within the Australian Autism Biobank. We identified lipids associated with ASD diagnosis (n = 8), sleep disturbances (n = 20) and cognitive function (n = 8) and found that long-chain polyunsaturated fatty acids may causally contribute to sleep disturbances mediated by the FADS gene cluster. We explored the interplay of environmental factors with neurodevelopment and the lipidome, finding that sleep disturbances and unhealthy diet have a convergent lipidome profile (with potential mediation by the microbiome) that is also independently associated with poorer adaptive function. In contrast, ASD lipidome differences were accounted for by dietary differences and sleep disturbances. We identified a large chr19p13.2 copy number variant genetic deletion spanning the LDLR gene and two high-confidence ASD genes (ELAVL3 and SMARCA4) in one child with an ASD diagnosis and widespread low-density lipoprotein-related lipidome derangements. Lipidomics captures the complexity of neurodevelopment, as well as the biological effects of conditions that commonly affect quality of life among autistic people.
Clinical implications of choroidal vascular brightness using ultra-widefield indocyanine green angiography in polypoidal choroidal vasculopathy
Authors: Jeong Areum , Yao Xue , Lee Kyungmin , Park Sang Hyun , Sagong Min 
Published: April 2023

AbstractPolypoidal choroidal vasculopathy (PCV) is characterized by choroidal vascular abnormalities including polypoidal lesion and branching vascular networks. Not only choroidal structural changes, but also choroidal hyperpermeability and congestion are also thought to be involved in pathogenesis of PCV. We investigated choroidal vascular brightness intensity (CVB) using ultra-widefield indocyanine green angiography (UWF-ICGA) images and analyzed its association with clinical features in patients with PCV. In this study, 33 eyes with PCV and 27 eyes of age-matched controls were included. CVB was measured by extracting the enhanced pixels of choroidal vessels after the reference brightness across the images was adjusted to be uniform. Associations between choroidal vascular features and the clinical features of PCV were also determined. The mean CVB was higher in PCV than control eyes, regardless of the segmented region (all p < 0.001). CVB was also higher at the posterior pole than at the periphery, and the inferior quadrants were brighter than the superior quadrants in both the PCV and control group (all p < 0.05). In affected eyes, CVB was higher than in unaffected fellow eyes at the posterior pole, whereas there was no difference at the periphery. Posterior pole CVB correlated significantly with subfoveal choroidal thickness (r = 0.502, p = 0.005), polyp number (r = 0.366 p = 0.030), and the greatest linear dimension (r = 0.680, p = 0.040). Greatest linear dimension was positively correlated with CVB at posterior pole (p = 0.040), whereas SFCT or CVD in all regions didn't show the significant correlation. The UWF ICGA results showed an increase in CVB at the inferior quadrants and posterior pole, suggesting venous outflow congestion in PCV eyes. CVB might provide more substantial information on the phenotype than other choroidal vascular features.
The impact of selected abiotic factors on Artemia hatching process through real-time observation of oxygen changes in a microfluidic platform
Authors: Dey Preyojon , Bradley Terence M. , Boymelgreen Alicia 
Published: April 2023

AbstractCurrent studies on abiotic impacts on Artemia, a crustacean which is widely used in aquaculture, and ecotoxicology, often focus on endpoint analysis (e.g., hatching rates, survival). Here, we demonstrate that a mechanistic understanding can be obtained through measurement of oxygen consumption in real-time over an extended time period in a microfluidic platform. The platform enables high level control of the microenvironment and direct observation of morphological changes. As a demonstration, temperature and salinity are chosen to represent critical abiotic parameters that are also threatened by climate change. The hatching process of Artemia consists of four different stages: hydration, differentiation, emergence, and hatching. Different temperatures (20, 35, and 30 °C) and salinities (0, 25, 50, and 75 ppt) are shown to significantly alter the duration of hatching stages, metabolic rates, and hatchability. Specifically, the metabolic resumption of dormant Artemia cysts was significantly enhanced at higher temperatures and moderate salinity, however, the time needed for this resumption was only dependent on higher temperatures. Hatchability was inversely related to the duration of the differentiation stage of hatching, which persisted longer at lower temperatures and salinities. The current approach of investigation of metabolism and corresponding physical changes can be employed to study hatching processes of other aquatic species, even those with low metabolic rate.
Modified minimum principal stress estimation formula based on Hoek–Brown criterion and equivalent Mohr–Coulomb strength parameters
Authors: Song Yanhui , Feng Man , Chen Peng 
Published: April 2023

AbstractThe most critical parameter for determining equivalent values for the Mohr–Coulomb friction angle and cohesion from the nonlinear Hoek–Brown criterion is the upper limit of confining stress. For rock slopes, this value is the maximum value of the minimum principal stress ($$\sigma_{3,\max }^{\prime }$$

σ

3
,
max

′

) on the potential failure surface. The existing problems in the existing research are analyzed and summarized. Using the finite element method (FEM), the location of potential failure surfaces for a wide range of slope geometries and rock mass properties are calculated using the strength reduction method, and a corresponding finite element elastic stress analysis was carried in order to determine $$\sigma_{3,\max }^{\prime }$$

σ

3
,
max

′

of the failure surface. Through a systematic analysis of 425 different slopes, it is found that slope angle (β) and geological strength index (GSI) have the most significant influence on $$\sigma_{3,\max }^{\prime }$$

σ

3
,
max

′

while the influence of intact rock strength and the material constant $$m_{i}$$

m
i

are relatively small. According to the variation of $$\sigma_{3,\max }^{\prime }$$

σ

3
,
max

′

with different factors, two new formulas for estimating $$\sigma_{3,\max }^{\prime }$$

σ

3
,
max

′

are proposed. Finally, the proposed two equations were applied to 31 real case studies to illustrate the applicability and validity.