bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus
Authors: Jiang Yu-Xi , Zheng Gao-Fan , Xin Xiu-Juan , Wu Hui , Ma Jun-Ying , Ju Jian-Hua , Zhao Ming , Wang Ruida , An Fa-Liang L. 
Published: June 2023

Abstract
Background
With the invasion of green tides and the increase of urban green areas worldwide, multimillion tons of Enteromorpha need to be reutilized. Ilamycins, novel cyclic heptapeptides with significant anti-TB activities, are isolated from Streptomyces atratus SCSIO ZH16, a deep-sea-derived strain. It is crucial to develop an efficient production process that can utilize inexpensive nitrogen sources to achieve massive production of ilamycins.
Results
In this study, Enteromorpha prolifera powder is considered a promising biomass resource for the production of commercial chemical products production. By using E. prolifera powder as a nitrogen source, the production of ilamycins reached 709.97 mg/L through optimization of the nitrogen source using the engineered strain S. atratus SCSIO ZH16 ΔR. After mutant strain constructions and tests, S. atratus SCSIO ZH16 ΔR::bldD was found to be a more suitable strain for utilizing E. prolifera powder. Furthermore, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively, in a 5-L bioreactor.
Conclusions
This study suggests that E. prolifera is a promising and eco-friendly nitrogen source for the production of ilamycins. Engineered strain S. atratus SCSIO ZH16 ΔR::bldD was found to be a more suitable strain for utilizing E. prolifera powder.
NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage
Authors: Chen Ping , Liu Xue-Yan , Lin Mou-Hui , Li Yu-Xi , Kang De-Zhi , Ye Zu-Cheng , Lin Qing-Song 
Published: June 2023

Abstract
Background
Subarachnoid hemorrhage (SAH) causes significant long-term neurocognitive dysfunction, which is associated with hippocampal neuroinflammation. Growing evidence have shown that astrocytes played a significant role in mediating neuroinflammation. Recently, in vivo reprogramming of astrocytes to neurons by NeuroD1 or PTBP1 administration have generated a lot of interests and controversies. While the debates centered on the source of neurogenesis, no attention has been paid to the changes of the astrocytes-mediated neuroinflammation and its impact on endogenous neurogenesis after NeuroD1 administration.
Methods
80 adult male C57BL/6 mice were used in this study. SAH was established by pre-chiasmatic injection of 100µl blood. AAV-NeuroD1-GFP virus was injected to the hippocampus 3 days post-SAH. Neurocognitive function, brain water content, in vivo electrophysiology, Golgi staining, western blot and immunofluorescent staining were assessed at day 14 post-virus injection.
Results
NeuroD1 administration dramatically attenuated reactive astrocytes-mediated neuroinflammation by reversing neurotoxic A1 astrocytes transformation, decreasing the secretion of neuroinflammatory cytokines, and reducing the activation of harmful microglia. NeuroD1 treatment significantly reversed the brain-blood barrier impairment and promoted the release of neurotrophic factors PTN, all of which contributed to the improvement of cellular microenvironment and made it more suitable for neurogenesis. Interestingly, besides neurogenesis in the hippocampus from cells transfected with NeuroD1 at the early phase of SAH, NeuroD1 administration significantly boosted the endogenous neurogenesis at the late phase of SAH, which likely benefited from the improvement of the neuroinflammatory microenvironment. Functionally, NeuroD1 treatment significantly alleviated neurocognitive dysfunction impaired by SAH.
Conclusions
NeuroD1 significantly promoted neurofunctional recovery by attenuating reactive astrocytes-mediated neuroinflammation and boosting neurogenesis decimated by SAH. Specifically, NeuroD1 efficiently converted transfected cells, most likely astrocytes, to neurons at the early phase of SAH, suppressed astrocytes-mediated neuroinflammation and boosted endogenous neurogenesis at the late phase of SAH.
Radiation effect mediated by divalent cations in human lung cancer cells in vitro
Authors: Qu Hongdao , Zhang Yadong , He Minyuan , Wang Jinbao 
Published: June 2023

Abstract
Radiotherapy has become a major treatment option for small cell lung carcinoma (SCLC) and locally advanced non SCLC (NSCLC). Moreover, divalent cations show a potential role in cancer therapy, which have become attractive nowadays. To explore the potential combination role of irradiation with divalent cations treatment in human lung cancer cells in vitro, human NSCLC A549 and SCLC NCI-H446 cells were irradiated with 2 Gy X-rays under 1 mM calcium (Ca2+), magnesium (Mg2+) or zinc (Zn2+) treatments. It was found that the Ca2+ and Mg2+ could inhibit activating ERK pathway, blocking cell proliferation, enhancing DNA damage, suppressing DNA repair capacity, strengthening p53 activation and increasing apoptosis induced by irradiation in lung cancer cells. On opposite, Zn2+ showed a negative regulation in radiation effect. Moreover, Ca2+, Mg2+ and Zn2+ could inhibit p38 activation and cell invasion induced by irradiation. These results suggest that divalent cations of Ca2+ and Mg2+ could potentiate radiation effect in lung cancer cells, which warrants further investigation for this combination effect of irradiation and divalent cations in potential clinical applications.
Brain Plasticity in Neonatal Brachial Plexus Palsies: Quantification and Comparison with Adults Brachial Plexus Injuries
Authors: Socolovsky Mariano , Masi Gilda di , Bonilla Gonzalo , Lovaglio Ana , Battaglia Danilo , Rosler Roberto , Malessy Martijn 
Published: June 2023

Abstract
Purpose
to compare two populations of brachial plexus palsies, one neonatal (NBPP) and the other traumatic (NNBPP) who underwent different nerve transfers, using the Plasticity Grading Scale (PGS) for detecting differences in brain plasticity between both groups.
Methods
To be included, all patients had to have undergone a nerve transfer as the unique procedure to recover one lost function. The primary outcome was the PGS score. We also assessed patient compliance to rehabilitation using the Rehabilitation Quality Scale (RQS). Statistical analysis of all variables was performed. A p ≤ 0.050 set as criterion for statistical significance.
Results
a total of 153 NNBPP patients and 35 NBPP babies (with 38 nerve transfers) met the inclusion criteria. The mean age at surgery of the NBPP group was 9 months (SD 5.42, range 4 to 23 months. The mean age of NNBPP patients was 22 years (SD 12 years, range 3 to 69). They were operated around sixth months after the trauma. All transfers performed in NBPP patients had a maximum PGS score of 4. This was not the case for the NNBPP population, that reached a PGS score of 4 in approximately 20% of the cases. This difference was statistically significant (p < 0.001). The RQS was not significantly different between groups
Conclusion
We found that babies with NBPP have a significantly greater capacity for plastic rewiring than adults with NNBPP. The brain in the very young patient can process the changes induced by the peripheral nerve transfer better than in adults.
Preparation and Anti-Triple-Negative Breast Cancer Cell Effect of a Nanoparticle for the Codelivery of Paclitaxel and Gemcitabine
Authors: Yang Fan , Fan Zehui , Zhang Lixia , He Yanjuan , Hu Run , Xiang Jinkun , Fu Shiyang , Wang Guowei , Wang Jianlong , Tao Xiaojun , Zhang Pan 
Published: June 2023

Abstract
Amphiphilic polymers (HA-ANI) were prepared by grafting hyaluronic acid (HA) and 6-(2-nitroimidazole)hexylamine (ANI) and then self-assemble in water to form nanoparticles (NPs) that could be loaded with paclitaxel (PTX) and gemcitabine (GEM) by dialysis. Infrared spectroscopy and 1H-NMR indicated the successful synthesis of HA-ANI. Three different ratios of NPs were prepared by adjusting the ratios of hydrophilic and hydrophobic materials, and the particle size decreased as the ratio of hydrophilic materials increased. When HA:ANI=2.0:1, the nanoparticles had the smallest size distribution, good stability and near spherical shape and had high drug loading and encapsulation rates. In vitro release experiments revealed that NADPH could accelerate the drug release from NPs. Cellular uptake rate reached 86.50% at 6 h. The toxic effect of dual drug-loaded nanoparticles (P/G NPs) on MDA-MB-231 cells at 48 h was stronger than that of the free drug. The AO/EB double-staining assay revealed that a large number of late apoptotic cells appeared in the P/G NPs group, and the degree of cell damage was significantly stronger than that of the free drug group. In the cell migration assay, the 24 h-cell migration rate of the P/G NPs group was 5.99%, which was much lower than that of the free group (13.87% and 17.00%). In conclusion, MDA-MB-231 cells could effectively take up P/G NPs, while the introduction of the nano-codelivery system could significantly enhance the toxicity of the drug to MDA-MB-231 cells as well as the migration inhibition effect.
Housing Tenure and the Risk of Mortality among Older Adults in Japan: Are Those Living in Public Rental Housing Healthier than Those Living in Private Rental Housing? A 9-Year Cohort Study from the JAGES
Authors: Koga Chie , Saito Tami , Hanazato Masamichi , Kondo Naoki , Saito Masashige , Ojima Toshiyuki , Kondo Katsunori 
Published: June 2023

Abstract

Housing tenure is an important aspect to determine health. However, even though renters tend to have more socioeconomic disadvantages than homeowners, the mortality risk between private and public renters compared with homeowners remains unclear. Japanese public rented housing, such as the Urban Renaissance Agency, has been developed for supplying an adequate living environment since 1950s. This study aimed to examine the mortality risk among older Japanese residents living in private and public rented houses compared with those living in owner-occupied houses using 9-year follow-up data. This study drew upon a 9-year follow-up of participants in the Japan Gerontological Evaluation Study, a population-based cohort study of Japanese independent adults aged ≥65 years. Mortality from 2010 to 2019 was analyzed for 44,007 respondents. Housing tenure was defined by a questionnaire. Cox regression models were used for calculating the hazard ratio for mortality. Bonferroni correction was used to account for multiple testing between rental houses. Overall, 10,638 deaths occurred during the follow-up period. Compared with housing owners, all rental housing groups had a significantly higher risk of mortality. Among renters, participants who lived in public rental housing had the lowest risk of mortality even after adjusting for sociodemographic characteristics, health status, social status, and environmental status. Multiple testing among renters with Bonferroni correction showed that public renters had 0.80 times (p = 0.0001) lower mortality risk than private renters. Although Japanese older adults living in public rental housing had a higher mortality risk than homeowners, this risk was lower than that among private renters. A positive neighborhood environment based on well-planned urban development may have contributed to this result. The results suggest that planned urban development lowers the risk of mortality in older renters in Japan.
Expression profile analysis of muscle growth regulation genes and effects of water flow stress on their expression levels in zebrafish
Authors: Yuan Xiudan , Tao Leiting , Hu Xiaoli , Lin Ruoyu , Yang Jingping , Feng Mengzhe , Peng Mei , Liu Wenbin , Xiao Yamei 
Published: June 2023

Abstract

Exercise can have beneficial effects on fish. However, there is few studies on the expression profile analysis of muscle growth regulation genes and impact of water flow stress on expression levels of the muscle genes in fish. In this study, myogenic regulatory genes (MyoD), myostatin-regulated genes (Mstnb), myoblast development-related genes (DesminandMYHC)were displayed high expression in muscle tissues of adult zebrafish.MyoD,MyoG, MRF4andMYHCare higher expression at the muscle effect stage embryos. And we explore that the 30 days old is critical muscle growth period in zebrafish. Furthermore, water flow stress can enhance the expression levels of myogenic regulatory factors (eg.MyoD, MyoG) and myoblast development-related genes (Pax7, Desmin) in the zebrafish trunk dorsal muscle, while the mRNA levels of myostatin regulatory genes, such asMstnaandMstnb, are significantly down-regulated. Our results suggest that appropriate water flow stressing for 1–2 weeks could promote zebrafish muscle growth, while zebrafish may be in motor fatigue after four weeks of water flow stress. This research provides a foundation for further exploration of fish exercise physiology and adaptation, and is significant for fish farming and conservation.
Heat Conductance of the Quantum Hall Bulk
Authors: Heiblum Moty , Melcer Ron , Gil Avigail , Umansky Vladimir , Oreg Yuval , Stern Ady , Berg Erez 
Published: June 2023

Abstract
The Quantum Hall Effect (QHE) is the prototypical realization of a topological state of matter. It emerges from a subtle interplay between topology, interactions, and disorder. The disorder enables the formation of localized states in the bulk that stabilize the quantum Hall states with respect to the magnetic field and carrier density. Still, the details of the localized states and their contribution to transport remain beyond the reach of most experimental techniques. Here, we describe an extensive study of the bulk's heat conductance. Using a novel 'multi-terminal' device, we separate the longitudinal thermal conductance (due to bulk's contribution) κxx T from the two-terminal value κ2T T, by eliminating the contribution of the edge modes. We find that when the field is tuned away from the conductance plateau center, the electronic states of the bulk conduct heat efficiently while the bulk remains electrically insulating. For fragile fractional states, such as the non-Abelian ν=5/2, we observe a finite κxx T throughout the plateau. We identify the localized states as the cause of the finite κxx T and propose a theoretical model which qualitatively explains our findings.
Design, Synthesis, and Biological Evaluation of New Biaryl Derivatives of Cycloalkyl diacetamide Bearing Chalcone Moiety as type II c-MET Kinase Inhibitors
Authors: Salarinejad Somayeh , Seyfi Soheila , Hayashi Seiko , Moghimi Setareh , Toolabi Mahsa , Taslimi Parham , Firoozpour Loghman , Usui Takeo , Foroumadi Alireza 
Published: June 2023

Abstract
Many human cancers have been associated with the deregulation of the mesenchymal-epithelial transition factor tyrosine kinase (MET) receptor, a promising drug target for anticancer drug discovery. Herein, we report the discovery of a novel structure of potent chalcone-based derivatives type II c-Met inhibitors which are comparable to Foretinib (IC50 = 14 nM) as a potent reference drug. Based on our design strategy, we also expected an anti-tubulin activity for the compounds. However, the weak inhibitory effects on microtubules were confirmed by cell cycle analyses implicated that the observed cytotoxicity against HeLa cells probably was not derived from tubulin inhibition. Compounds 14q and 14k with IC50 values of 25 nM and 46 nM, respectively, demonstrated favorable inhibition of MET kinase activity, and desirable bonding interactions in the ligand-MET enzyme complex stability in molecular docking studies.
Bone transport using a unilateral versus circular external fixator over an intramedullary nail in the treatment of tibial bone defect caused by infection: a retrospective comparative study
Authors: Maimaiti Xiayimaierdan , Liu Kai , Yusufu Aihemaitijiang , Xie Zengru 
Published: June 2023

Abstract
Background
The purpose of the study was to compare the clinical efficacy of bone transport with either circular or unilateral external fixators over an intramedullary nail (EFOIN) in the treatment of tibial bone defects caused by infection.
Methods
Between May 2010 and January 2019, clinical and radiographic data were collected and analyzed for patients with bone defects caused by infection. Thirteen patients underwent bone transport using a unilateral external fixator over an intramedullary nail (Group A), while 12 patients were treated with a circular external fixator over an intramedullary nail (Group B). The bone and functional outcomes of both groups were assessed and compared using the Association for the Study and Application of the Method of the Ilizarov (ASAMI) criteria, and postoperative complications were evaluated according to the Paley classification.
Results
A total of 25 patients were successfully treated with bone transport using EFOIN, with a mean follow-up time of 31.63 ± 5.88 months. There were no significant statistical differences in age, gender, previous surgery per patient, duration of infection, DS, EFT, EFI, BUT, and follow-up time between Group A and Group B (P > 0.05). However, statistically significant differences were observed in operation time and intraoperative blood loss between the two groups (P < 0.05). The excellent and good rate of bone and functional results were higher in Group A compared to Group B (76.9% vs 75% and 84.6% vs 58.3%). Statistically significant differences were observed in functional results and complication per patient between the two groups (P < 0.05).
Conclusions
Bone transport using a combined technique of EFOIN proved to be an effective method in treating tibial bone defects caused by infection. In comparison to circular external fixators, bone transport utilizing a unilateral external fixator over an intramedullary nail resulted in fewer complications and better functional outcomes.
Associating White Matter Microstructural Integrity and Improvements in Reactive Stepping in People with Parkinson’s Disease
Authors: Andrew Sean Monaghan , Ofori Edward , Brett W Fling , Daniel S. Peterson 
Published: June 2023

Abstract
Reactive steps are rapid responses after balance challenges. People with Parkinson's Disease demonstrate impaired reactive stepping, increasing fall risk. Although PwPD can improve steps through practice, the neural mechanisms contributing to improved reactive stepping in people with PD are poorly understood. This study investigated white-matter correlates of responsiveness to reactive step training in people with PD. Participants completed an eighteen-week multiple-baseline study consisting of two baseline assessments (B1 and B2) before training, a two-week, six-session training protocol, and two post-training assessments (immediate; P1) and two months after training (P2). Each assessment consisted of 3 backward reactive step trials. Outcomes included the anterior-posterior margin of stability, step length, and step latency. Tract-Based Spatial Statistics were performed to correlate white-matter microstructural integrity (fractional anisotropy and radial diffusivity) with retained improvements in reactive stepping at the two-month follow-up (P2–B2). Complete datasets were available from 22 participants. Greater retention of step length was associated with increased fractional anisotropy (better white-matter integrity) within the left anterior corona radiata (r = 0.54, p < 0.01), left posterior thalamic radiation (r = 0.54, p < 0.01), and right (r = 0.43, p = 0.04) and left (r = 0.0.40, p = 0.06) superior longitudinal fasciculi. Greater retention of step latency improvements was associated with lower radial diffusivity (greater white-matter integrity) within the left posterior (r = 0.60, p < 0.01) and anterior corona radiata (r = 0.61, p < 0.01). These findings highlight the importance of white-matter microstructural integrity in motor learning and retention processes in PD and may inform the development of targeted interventions to improve balance in people with PD.
Multifocal Metastatic Spread of Prostate Adenocarcinoma: A Case Report Involving Lung and Brain Infiltration
Authors: Bansro Varinder , Chhabra Gurdeep , Lee Heather , Parkash Jasmita , Siddiqi Sarwat 
Published: June 2023

Abstract
Background:
Prostate cancer is a prevalent malignancy in males, particularly in older individuals and non-Hispanic Black men. Adenocarcinomas represent most prostate cancers, while other histological types are less common. Metastases from prostate cancer primarily involve bones and lymph nodes, with isolated lung and brain metastases being extremely uncommon. Understanding prostate cancer's clinical characteristics and behavior with atypical metastatic patterns is crucial for accurate diagnosis and optimal treatment strategies.
Case Presentation:
We present a case of a 60-year-old patient with high-grade Gleason prostate cancer, initially presenting with negative PSA levels. Despite the absence of bone involvement, the patient developed metastases to both the lung and brain, representing an unusual spread pattern. Detailed clinical features, imaging findings, diagnostic workup, and treatment interventions are described to provide a comprehensive understanding of this exceptional case.
Conclusion:
This case report highlights the rarity and clinical significance of prostate cancer metastases to the lung and brain without bone involvement. Isolated lung and brain metastases from prostate cancer are infrequent occurrences, and their diagnosis and management pose considerable challenges. Clinicians should be aware of these potential metastatic patterns, especially when encountering unusual clinical presentations or imaging findings in prostate cancer patients. Further research and case reports are warranted to enhance our knowledge of these rare metastatic events and optimize treatment approaches for patients with prostate cancer and atypical metastases.