bluuebunny/crossref_metadata_2025_split huggingface dataset:
lncRNA SND1‐IT1 delivered via intracerebral hemorrhage‐derived exosomes affect the growth of human microglia by regulating the miR‐124‐3p/MTF1 axis
Authors: Wang Jian , Teng Fei , Liu Shuhan , Pan Xiaoqiang , Yang Bo , Wu Wei 
Published: December 2022

AbstractIn this study, we investigated the effects of long noncoding RNA (lncRNA) SND1‐IT1 on human microglia (HMC3 cells) delivered by intracerebral hemorrhage (ICH)‐derived exosomes (ICH‐exos) as well as a competitive endogenous RNA (ceRNA) network. Exosomes obtained from ICH plasma were characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blot. RNA sequencing was performed to study the lncRNA transcriptome from ICH‐exos and the healthy control‐derived exosomes (HC‐exos) and differentially expressed lncRNAs (DE‐lncRNAs) were identified. HMC3 cells were treated with ICH‐exos or transfected with pcDNA3.1‐SND1‐IT1, and then cell viability and apoptosis were measured. The ceRNA network (lncRNA SND1‐IT1/miR‐124‐3p/messenger RNA MTF1) was chosen for further investigation. NTA, TEM, and western blot showed that exosomes were successfully separated and could be absorbed by HMC3 cells. The expression of lncRNA SND1‐IT1 in ICH‐exos was significantly higher than that of HC‐exos (p < 0.05). In addition, lncRNA SND1‐IT1 overexpression and ICH‐exos significantly inhibited cell viability and enhanced apoptosis. A total of 162 DE‐lncRNAs were identified by sequencing, and a ceRNA network was constructed. The dual‐luciferase reporter gene indicated that lncRNA SND1‐IT1, miR‐124‐3p, and MTF1 interacted with each other. Cell experiments showed that lncRNA SND1‐IT1 affected the growth of HMC3 cells through miR‐124‐3p/MTF1. In conclusion, ICH‐exos delivered lncRNA SND1‐IT1 to HMC3 cells, and exosomal lncRNA SND1‐IT1 can regulate cell viability and apoptosis to influence HMC3 cell growth via the SND1‐IT1/miR‐124‐3p/MTF1 axis.
Deep learning‐based assessment of body composition and liver tumour burden for survival modelling in advanced colorectal cancer
Authors: Keyl Julius , Hosch René , Berger Aaron , Ester Oliver , Greiner Tobias , Bogner Simon , Treckmann Jürgen , Ting Saskia , Schumacher Brigitte , Albers David , Markus Peter , Wiesweg Marcel , Forsting Michael , Nensa Felix , Schuler Martin , Kasper Stefan , Kleesiek Jens 
Published: December 2022

AbstractBackgroundPersonalized therapy planning remains a significant challenge in advanced colorectal cancer care, despite extensive research on prognostic and predictive markers. A strong correlation of sarcopenia or overall body composition and survival has been described. Here, we explore whether automated assessment of body composition and liver metastases from standard of care CT images can add to clinical parameters in personalized survival risk prognostication.MethodsWe retrospectively analysed clinical imaging data from 85 patients (50.6% female, mean age 58.9 SD 12.2 years) with colorectal cancer and synchronous liver metastases. Pretrained deep learning models were used to assess body composition and liver metastasis geometry from abdominal CT images before the initiation of systemic treatment. Abdominal muscle‐to‐bone ratio (MBR) was calculated by dividing abdominal muscle volume by abdominal bone volume. MBR was compared with body mass index (BMI), abdominal muscle volume, and abdominal muscle volume divided by height squared. Differences in overall survival based on body composition and liver metastasis parameters were compared using Kaplan–Meier survival curves. Results were correlated with clinical and biomarker data to develop a machine learning model for survival risk prognostication.ResultsThe MBR, unlike abdominal muscle volume or BMI, was significantly associated with overall survival (HR 0.39, 95% CI: 0.19–0.80, P = 0.009). The MBR (P = 0.022), liver metastasis surface area (P = 0.01) and primary tumour sidedness (P = 0.007) were independently associated with overall survival in multivariate analysis. Body composition parameters did not correlate with KRAS mutational status or primary tumour sidedness. A prediction model based on MBR, liver metastasis surface area and primary tumour sidedness achieved a concordance index of 0.69.ConclusionsAutomated segmentation enables to extract prognostic parameters from routine imaging data for personalized survival modelling in advanced colorectal cancer patients.
Retinal pigment epithelium extracellular vesicles are potent inducers of age‐related macular degeneration disease phenotype in the outer retina
Authors: Kurzawa‐Akanbi Marzena , Whitfield Phillip , Burté Florence , Bertelli Pietro Maria , Pathak Varun , Doherty Mary , Hilgen Birthe , Gliaudelytė Lina , Platt Mark , Queen Rachel , Coxhead Jonathan , Porter Andrew , Öberg Maria , Fabrikova Daniela , Davey Tracey , Beh Chia Shyan , Georgiou Maria , Collin Joseph , Boczonadi Veronika , Härtlova Anetta , Taggart Michael , Al‐Aama Jumana , Korolchuk Viktor I , Morris Christopher M , Guduric‐Fuchs Jasenka , Steel David H , Medina Reinhold J , Armstrong Lyle , Lako Majlinda 
Published: December 2022

AbstractAge‐related macular degeneration (AMD) is a leading cause of blindness. Vision loss is caused by the retinal pigment epithelium (RPE) and photoreceptors atrophy and/or retinal and choroidal angiogenesis. Here we use AMD patient‐specific RPE cells with the Complement Factor H Y402H high‐risk polymorphism to perform a comprehensive analysis of extracellular vesicles (EVs), their cargo and role in disease pathology. We show that AMD RPE is characterised by enhanced polarised EV secretion. Multi‐omics analyses demonstrate that AMD RPE EVs carry RNA, proteins and lipids, which mediate key AMD features including oxidative stress, cytoskeletal dysfunction, angiogenesis and drusen accumulation. Moreover, AMD RPE EVs induce amyloid fibril formation, revealing their role in drusen formation. We demonstrate that exposure of control RPE to AMD RPE apical EVs leads to the acquisition of AMD features such as stress vacuoles, cytoskeletal destabilization and abnormalities in the morphology of the nucleus. Retinal organoid treatment with apical AMD RPE EVs leads to disrupted neuroepithelium and the appearance of cytoprotective alpha B crystallin immunopositive cells, with some co‐expressing retinal progenitor cell markers Pax6/Vsx2, suggesting injury‐induced regenerative pathways activation. These findings indicate that AMD RPE EVs are potent inducers of AMD phenotype in the neighbouring RPE and retinal cells.
Risk for incident comorbidities, nonhepatic cancer and mortality in acute hepatic porphyria: A matched cohort study in 1244 individuals
Authors: Lissing Mattias , Vassiliou Daphne , Floderus Ylva , Harper Pauline , Yan Jacinth , Hagström Hannes , Sardh Eliane , Wahlin Staffan 
Published: December 2022

AbstractThe acute hepatic porphyrias (AHP) are associated with long‐term complications such as primary liver cancer, hypertension, and chronic kidney disease. Data on other related comorbidities are scarce. In this register‐based, matched cohort study, we assessed the risks of nonhepatic cancers, cardiovascular diseases, renal diseases, psychiatric disorders, and mortality in relation to porphyria type, sex, and biochemical disease activity. All patients in the Swedish porphyria register with a verified AHP diagnosis during 1987–2015 were included. The biochemical activity of acute intermittent porphyria was assessed using recorded maximal urinary porphobilinogen (U‐PBG). Data on incident comorbidities and mortality were collected from national health registries. Cumulative incidences, rates, and hazards were compared to reference individuals from the general population, matched 1:10 by age, sex, and county. We identified 1244 patients with AHP with a median follow‐up of 19 years. Health registries identified 149 AHP‐subjects (12.0%) with nonhepatic cancer, similar to 1601 (13.0%) in the matched reference population (n = 12 362). Patients with AHP had a higher risk of kidney cancer (0.8% vs. 0.2%, p < 0.001), hypertension, and chronic kidney disease but no increase in risk for cardiovascular disease, except for cerebrovascular disease in patients with elevated U‐PBG, (aHR = 1.40 [95% CI:1.06–1.85]). Mortality risk during follow‐up was higher among patients with AHP (21% vs. 18%, p = 0.001), and associated with primary liver cancer, female sex, and biochemical activity. In conclusion, AHP is associated with an increased risk of kidney cancer, hypertension, chronic kidney disease, and mortality but not with cardiovascular disease or other nonhepatic cancers.
Transformational Leadership and Sick Leave: A Randomized Controlled Trial
Authors: Hauth Tobias , Peiró José M. , Mesa Juan M. 
Published: December 2022

Although prior research has established an association between transformational leadership and sick leave, there is still no clear evidence for a causal relationship between the two constructs. The present study contributes to this quest by developing and evaluating the effectiveness of a transformational leadership intervention in reducing employee sick leave. One hundred and seventeen leaders from two Spanish organizations were randomly assigned to either the intervention (n = 54) or control condition (n = 63). An ANCOVA analysis of 6 months of pre and post‐test sick leave data was used to examine the impact of the intervention. Results confirmed the effectiveness of the intervention in reducing total subordinate sick days, sick days due to long‐term spells and sick days of younger employees. No significant effect was found for follower sick days due to short‐term spells and sick days in older employees. As levels of sick leave rise, an evidence‐based and actionable tool is provided for organizations seeking to improve employee health.
Omicron infection increases IgG binding to spike protein of predecessor variants
Authors: Mahalingam Gokulnath , Periyasami Yogapriya , Arjunan Porkizhi , Subaschandrabose Rajesh K. , Mathivanan Tamil V. , Mathew Roshlin S. , Devi Ramya K. T. , Premkumar Prasanna S. , Muliyil Jayaprakash , Srivastava Alok , Moorthy Mahesh , Marepally Srujan 
Published: December 2022

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) transmission in India in 2020–2022 was driven predominantly by Wild (Wuhan‐Hu‐1 and D614G), Delta, and Omicron variants. The aim of this study was to examine the effect of infections on the humoral immune response and cross‐reactivity to spike proteins of Wuhan‐Hu‐1, Delta, C.1.2., and Omicron. Residual archival sera (N = 81) received between January 2020 and March 2022 were included. Infection status was inferred by a positive SARS‐CoV‐2 RT‐PCR and/or serology (anti‐N and anti‐S antibodies) and sequencing of contemporaneous samples (N = 18) to infer lineage. We estimated the levels and cross‐reactivity of infection‐induced sera including Wild, Delta, Omicron as well as vaccine breakthrough infections (Delta and Omicron). We found an approximately two‐fold increase in spike‐specific IgG antibody binding in post‐Omicron infection compared with the pre‐Omicron period, whilst the change in pre‐ and post‐Delta infections were similar. Further investigation of Omicron‐specific humoral responses revealed primary Omicron infection as an inducer of cross‐reactive antibodies against predecessor variants, in spite of the weaker degree of humoral response compared to Wuhan‐Hu‐1 and Delta infection. Intriguingly, Omicron vaccine‐breakthrough infections when compared with primary infections, exhibited increased humoral responses against RBD (7.7‐fold) and Trimeric S (Trimeric form of spike protein) (34.6‐fold) in addition to increased binding of IgGs towards previously circulating variants (4.2 ‐ 6.5‐fold). Despite Delta breakthrough infections showing a higher level of humoral response against RBD (2.9‐fold) and Trimeric S (5.7‐fold) compared to primary Delta sera, a demonstrably reduced binding (36%–49%) was observed to Omicron spike protein. Omicron vaccine breakthrough infection results in increased intensity of humoral response and wider breadth of IgG binding to spike proteins of antigenically‐distinct, predecessor variants.
Originality vs. Appropriateness: The Moderating Role of Culture on the Effect of Instructional Focus on Individual and Team Creativities
Authors: Zheng Mei , Niu Weihua , Wang Wei , Cheng Li , Ma Tianjiao , Park Ji Hoon 
Published: December 2022

ABSTRACTCreativity is one of the essential skills for the 21st century. Although current advancement in the research converges on its dual features (i.e., originality and appropriateness) and the effect of instructional focus, little is known about how culture and work modality (i.e., individual or collaborative) play a role in the effect. This study examined the moderating role of culture on the impact of instructional focus on creative performance at both individual and team levels. We recruited 144 participants (72 from the United States and 72 from China) to form 48 working teams of trios, half of which were instructed to focus on originality while the other half to focus on appropriateness. Our results revealed a main effect of instructional focus on creativity only at the team level but not at the individual level. More importantly, we found that the individualistic culture yielded the best creative performance with individual work modality when instructed to focus on originality, whereas the collectivistic culture yielded the best creative performance with team work modality when instructed to focus on appropriateness. Theoretical and practical implications are discussed.
Effects of pea protein content and extrusion types on physicochemical properties and texture characteristic of meat analogs
Authors: Zhang Yu , Ryu Gi‐Hyung 
Published: December 2022

AbstractBackgroundWith the change in consumers' lifestyles, the global demand for meat analogs is growing rapidly. However, soy has many drawbacks as a raw material to produce meat analogs, such as its allergy potential (glycinin and β‐conglycinin) and some unpredictable alterations in genetic DNA, proteins, and biochemical processing due to genetic modification. This paper aims to study the physicochemical and textural properties of meat analogs obtained under different pea protein contents and extrusion types to explore the potential of applying pea protein to meat analogs.ResultsHigh‐moisture meat analog (HMMA) is superior to low‐moisture meat analog (LMMA) based on the physical properties (integrity index and nitrogen solubility index) and texture properties (texture profile analysis and cutting strength). The higher the amount of pea protein added, the higher the degree of sample texturization. At low‐moisture extrusion cooking (LMEC), higher isolated pea protein content is accompanied by lower water holding capacity and higher texturization. Hydrophobic interactions play a slightly greater role than disulfide bonds in stabilizing the protein structure of the extrudates. The type of extrusion significantly affected the free sulfhydryl and disulfide bond content of the extrudate, while the source of raw material has an important relationship with protein solubility and free sulfhydryl content. Dodecyl sulfated gel electrophoresis (SDS‐PAGE) showed that most of the protein subunits remained after extrusion.ConclusionsExtrusion types and isolated pea protein contents showed significant effects on the properties of meat analogs, and isolated pea protein has great potential for application in the production of meat analogs.
LUMiC® endoprosthesis for pelvic reconstruction: A Canadian experience
Authors: Rizkallah Maroun , Ferguson Peter C. , Basile Georges , Kim Paul , Werier Joel , Wilson David , Turcotte Robert 
Published: December 2022

AbstractBackground and ObjectivesThe LUMiC® prosthesis was introduced to reduce the mechanical complications encountered with periacetabular reconstruction after pelvic tumor resection. Few have evaluated the outcomes associated with its use.MethodsA retrospective study from five Orthopedic Oncology Canadian centers was conducted. All patients with a LUMiC® endoprosthesis were included. Their charts were reviewed for surgical and functional outcomes.ResultsA total of 16 patients were followed for 28 months (3–60). A total of 12 patients (75%) had a LUMiC® after a resection of a primary sarcoma. Mean surgical time was 555 min. Four patients (25%) had a two‐stages procedure. MSTS score was 60.3 preoperatively and 54.3 postoperatively. Patients got a dual mobility bearing and the silver coated implant was used in 7 patients (43.7%). Five patients (31.3%) underwent capsular reconstruction using a fabric. Silver‐coating was not found to reduce infection risk (p = 0.61) and capsuloplasty did not prevent dislocation (p = 0.6). Five patients had peroperative complications (31.3%). Eight patients (50%) had an infection including all four with two‐stages surgery. Dislocation occurred in five patients (31.3%) whereas no cases of aseptic loosening were reported. A total of 10 patients (62.5%) needed a reoperation.ConclusionLUMiC® endoprosthesis provides low rates of aseptic loosening on medium‐term follow‐up. Infection and dislocation are common complications but we were unable to show benefits of capsuloplasty and silver‐coated implants.
Enriched PUFA environment of Leishmania infantum promastigotes promotes the accumulation of lipid mediators and favors parasite infectivity towards J774 murine macrophages
Authors: Leroux Marine , Bouazizi‐Ben Messaoud Hana , Luquain‐Costaz Céline , Jordheim Lars P. , Le Faouder Pauline , Gustin Marie‐Paule , Aoun Karim , Lawton Philippe , Azzouz‐Maache Samira , Delton Isabelle 
Published: December 2022

AbstractLeishmania parasites are the causative agents of visceral or cutaneous leishmaniasis in humans and of canine leishmaniosis. The macrophage is the predilected host cell of Leishmania in which the promastigote stage is transformed into amastigote. We previously showed changes in the fatty acid composition (FA) of lipids in two strains of Leishmania donovani upon differentiation of promastigote to amastigote, including increased proportions of arachidonic acid (AA) and to a less extent of docosahexaenoic acid (DHA). Here, we carried out supplementation with AA or DHA on two Leishmania infantum strains, a visceral (MON‐1) and a cutaneous (MON‐24), to evaluate the role of these FA in parasite/macrophage interactions. The proportions of AA or DHA in total lipids were significantly increased in promastigotes cultured in AA‐ or DHA‐supplemented media compared to controls. The content of FA‐derived oxygenated metabolites was enhanced in supplemented strains, generating especially epoxyeicosatrienoic acids (11,12‐ and 14,15‐EET) and hydroxyeicosatetraenoic acids (5‐ and 8‐ HETE) from AA, and hydroxydocosahexaenoic acids (14‐ and 17‐HDoHE) from DHA. For both MON‐1 and MON‐24, AA‐supplemented promastigotes showed higher infectivity towards J774 macrophages as evidenced by higher intracellular amastigote numbers. Higher infectivity was observed after DHA supplementation for MON‐24 but not MON‐1 strain. ROS production by macrophages increased upon parasite infection, but only minor change was observed between control and supplemented parasites. We propose that under high AA or DHA environment that is associated with AA or DHA enrichment of promastigote lipids, FA derivatives can accumulate in the parasite, thereby modulating parasite infectivity towards host macrophages.
Global solutions to the damped MHD system
Authors: Zhai Xiaoping , Chen Zhi‐Min 
Published: December 2022

AbstractThe global existence of solutions to a damped MHD system in Besov spaces is obtained. This research is motivated by the open question [Wu et al., J. Nonlinear Sci. 25 (2015), 157–192, Remark 5.1] on the possibility of extension to the existence of global small solutions from a potential Banach space to a Besov space. The present result positively confirms the open question. What is more, the initial data are not required to be uniformly small.
Synthesis and Characterization of Alginate/Bentonite Hydrogels
Authors: Morant Mónica Alejandra , Horst María Fernanda , Giaroli María Carolina , Rodríguez Mario Humberto , Ninago Mario Daniel 
Published: December 2022

AbstractThe use of bentonite for organic dyes removal in the water system is an economical and highly efficient alternative against the usually applied purification methods. However, during the post‐treatment stage bentonite swells and forms highly stable colloidal suspensions, being difficult to separate from the aqueous system. In this work, calcium alginate hydrogels beads with 5 and 10% w/w of bentonite are synthetized and their capacity to adsorb methylene blue, as model dye, from aqueous media is evaluated. Structure and mechanical properties of hydrogels beads are determined, showing that hydrogels beads with bentonite present higher viscosity and hardness. Regarding methylene blue adsorption capacity, the effect the contact time (0–32 h) and the initial cadmium ion concentration (0.2–3.0 mg L−1) are studied employing hydrogel beads. The equilibrium adsorption time is reached at 300 min for all studied hydrogels and experimental data are fitted well with a pseudo‐second order kinetic model. As conclusion, alginate/bentonite hydrogel beads are accepted as a promising candidate to remove methylene blue dye from aqueous solutions.
Interaction Between Type I and II Coagents and Polybutadiene Isomers on Crosslinking Systems Using Organic Peroxide
Authors: Neumann Chaiane , Romanzini Daiane , Andrade Mara Z. , Francisquetti Edson L. 
Published: December 2022

AbstractThis study aims to evaluate the influence of types I and II coagents on different polybutadiene isomers vulcanized with organic peroxides. In this study, dicumyl peroxide (DCP) is used in association with coagents ethylene glycol dimethacrylate (EGDMA) and triallyl isocianurate (TAIC) in the crosslinking of high cis‐1,4‐polybutadiene and high vinyl‐1,2‐polybutadiene isomers. The results reveal that using a type I coagent, EGDMA increases the crosslink density as the amount of coagent is increased. The curing index of the samples is positively influenced by coagent type I, mainly when vinyl‐1,2‐polybutadiene is used.
Photochemical Nitric Oxide Release from S‐Nitrosothiol‐Functionalized Chitosan
Authors: de Almeida Herllan V. , Catori Daniele M. , da Silva Laura C. E. , de Oliveira Marcelo G. 
Published: December 2022

AbstractS‐nitrosothiols (RSNOs) are nitric oxide (NO) donor molecules with potential biomedical applications since they mimic NO biological functions, such as vasodilation and inflammatory process regulation. In this work, chitosan (CS), a natural biopolymer widely used as a biomaterial, is functionalized with thioglycolic acid (TGA) or mercaptosuccinic acid (MSA) in reactions mediated by a carbodiimide, yielding CS with thiol contents of 88 µmol g–1 (CS‐TGA) and 126 µmol g–1 (CS‐MSA). The subsequent S‐nitrosation of CS‐TGA and CS‐MSA generates the corresponding S‐nitroso derivatives, CS‐TGA(SNO) and CS‐MSA(SNO). The UV–Vis spectral changes associated with the S‐NO bond cleavage of CS‐TGA(SNO) and CS‐MSA(SNO), monitored in solution over 18 days of storage in a common refrigerator, reveal that these S‐nitroso CS derivatives display enhanced thermal stability in the dark, with half‐life values of 36.1 for CS‐TGA(SNO) and 16.1 days for CS‐MSA(SNO). Conversely, CS‐TGA(SNO) and CS‐MSA(SNO) can release NO photochemically on‐demand under visible light irradiation in a first‐order process triggered by photon absorption that leads to a half‐life of 33 min for both S‐nitroso CS. These results show that CS‐TGA(SNO) and CS‐MSA(SNO) may emerge as new CS‐based biomaterials for providing light‐triggered local NO release in biomedical applications.