bluuebunny/crossref_metadata_2025_split huggingface dataset:
CVD-associated SNPs with regulatory potential drive pathologic non-coding RNA expression
Authors: Zhu Chaonan , Baumgarten Nina , Wu Meiqian , Wang Yue , Das Arka Provo , Kaur Jaskiran , Ardakani Fatemeh Behjati , Duong Thanh Thuy , Pham Minh Duc , Duda Maria , Dimmeler Stefanie , Yuan Ting , Schulz Marcel H. , Krishnan Jaya 
Published: March 2023

Abstract
Background:
Cardiovascular diseases (CVDs) are the leading cause of death worldwide. Genome-wide association studies (GWAS) have identified many single nucleotide polymorphisms (SNPs) appearing in non-coding genomic regions in CVDs. The SNPs may alter gene expression by modifying transcription factor (TF) binding sites and lead to functional consequences in cardiovascular traits or diseases. To understand the underlying molecular mechanisms, it is crucial to identify which variations are involved and how they affect TF binding.
Methods:
The SNEEP (SNP exploration and analysis using epigenomics data) pipeline was used to identify regulatory SNPs, which alter the binding behavior of TFs and link GWAS SNPs to their potential target genes for six CVDs. The human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CMs), monoculture cardiac organoids (MCOs) and self-organized cardiac organoids (SCOs) were used in the study. Gene expression, cardiomyocyte size and cardiac contractility were assessed.
Results:
By using our integrative computational pipeline, we identified 1905 regulatory SNPs in CVD GWAS data. These were associated with hundreds of genes, half of them non-coding RNAs (ncRNAs), suggesting novel CVD genes. We experimentally tested 40 CVD-associated non-coding RNAs, among them RP11-98F14.11, RPL23AP92, IGBP1P1, and CTD-2383I20.1, which were upregulated in hiPSC-CMs, MCOs and SCOs under hypoxic conditions. Further experiments showed that IGBP1P1 depletion rescued expression of hypertrophic marker genes, reduced hypoxia-induced cardiomyocyte size and improved hypoxia-reduced cardiac contractility in hiPSC-CMs and MCOs.
Conclusions:
IGBP1P1 is a novel ncRNA with key regulatory functions in modulating cardiomyocyte size and cardiac function in our disease models. Our data suggest ncRNA IGBP1P1 as a potential therapeutic target to improve cardiac function in CVDs.
Comparison functions, operational laws and aggregation operators for ambiguous set: Application in solving multi-criteria group decision-making problem under ambiguous environment
Authors: Singh Pritpal 
Published: March 2023

Abstract
Ambiguous set has recently been proposed to represent inherent uncertainties, imprecision, and vague information. In this study, we generalize the concept of ambiguous set to a single-valued ambiguous number (SVAN). We propose many comparison functions and operational laws for SVANs. This study also introduces three new aggregation operators, such as the ambiguous weighted geometric operator (AWGO), the ambiguous ordered weighted geometric operator (AOWGO), and the ambiguous hybrid geometric operator (AHGO), which are used to aggregate input information in the form of SVANs. We discuss some significant properties of the operators along with numerical examples. Based on the AWGO and AHGO, we develop a method for solving the multi-criteria group decision-making (MCGDM) problem. Finally, a real-time example of an MCGDM problem associated with the selection of cars by customers is presented, and its solution is recommended using the proposed method.
miR-377 inhibits the tumorous behavior of prostate cancer cells by targeting MYC
Authors: Azimi Yasamin , Hajibabaei Sara , Azimi Ghazal , Rahimi-Jamnani Fatemeh , Azizi Masoumeh 
Published: March 2023

Abstract
Aims
The MYC gene is one of the regulatory and proto-oncogenic genes that is overexpressed in most prostate cancers. Studies have shown that abnormal expression of microRNAs is involved in the onset and development of many different types of human cancer, including prostate cancer.
Materials and methods
In this study, we first evaluated targeting the effect of miR-377 on MYC by luciferase assay. Real-time PCR was used to figure out whether miR-377 could decrease the target gene mRNAs in transfected PCa cell lines (PC3 and DU145). The effects of miR-377 on apoptosis cells, proliferation, cell cycle, and wound healing were analyzed.
Results
We showed that miR-377 targets MYC mRNA by luciferase reporter assay. A significant reduction in MYC mRNA level was detected, following miR-377 transfection in PC3 and DU145 cell lines. The higher levels of miR-377 in PCa cell lines induced apoptosis, reduced proliferation, and migration, and stopped the cell cycle.
Conclusion
All these data reveal that miR-377 functions as a tumor suppressor in PCa and may serve as a potential therapeutic target for the treatment of this cancer.
Laboratory induction of accelerated corrosion in rebars of reinforced concrete structures
Authors: COVATTI Leonardo , MEDEIROS Arthur , FILHO Luiz Carlos Pinto SILVA , BAPTISTA-PEREIRA Catarina , OLIVEIRA Matheus Pontes 
Published: March 2023

Abstract
The study of pathologies in the construction industry are rising and getting more focused in the field of reinforced concrete. In these types of structures, the most common pathologies are: excessive displacement, efflorescence, lack of concreting and cracks and/or crazing, which usually occur due the corrosion of rebars, or due to excess of water and poor cure, respectively. In this study, laboratory tests were performed to accelerate the corrosion process of steel samples. To achieve that, it was used the Tamori's methodology, the electrolytic corrosion. The tests were performed in ten circular reinforced concrete specimens and, due to equipment constrains and time limitations, the specimens were divided in two main groups. The cement used was the Brazilian Portland CP-V ARI and the rebar was CA-50 with a 6.3 mm nominal diameter. After applying a constant electric current in the steel rebars of the reinforced concrete specimens for a certain pre-defined period, it was possible to identify stains and fissures in the faces of the specimens, as well as to measure the corrosion that occurred. To measure the corrosion of the rebars of the specimens, the samples were fractured, the rebars were removed and they were later subject to a thermochemical bath. Finally, it was measured that the rebars had different percentages of corrosion loss when comparing with theoretical values from the literature.
Reduced steroid activation of elephant shark glucocorticoid and mineralocorticoid receptors after inserting four amino acids from the DNA-binding domain of lamprey corticoid receptor-1
Authors: Katsu Yoshinao , Zhang Jiawen , Baker Michael E. 
Published: March 2023

Abstract
Atlantic sea lamprey contains two corticoid receptors (CRs), CR1 and CR2, that are identical except for a four amino acid insert (Thr-Arg-Gln-Gly) in the CR1 DNA-binding domain (DBD). Steroids are stronger transcriptional activators of CR2 than of CR1 suggesting that the insert reduces the transcriptional response of lamprey CR1 to steroids. The DBD in elephant shark mineralocorticoid receptor (MR) and glucocorticoid receptor (GR), which are descended from a CR, lack these four amino acids, suggesting that a CR2 is their common ancestor. To determine if, similar to lamprey CR1, the presence of this insert in elephant shark MR and GR decreases transcriptional activation by corticosteroids, we inserted these four CR1-specific residues into the DBD of elephant shark MR and GR. Compared to steroid activation of wild-type elephant shark MR and GR, cortisol, corticosterone, aldosterone, 11-deoxycorticosterone and 11-deoxycortisol had lower transcriptional activation of these mutant MR and GR receptors, indicating that the absence of this four-residue segment in the DBD in wild-type elephant shark MR and GR increases transcriptional activation by corticosteroids.
Bioinformatics analysis of SH2D4A in Glioblastoma Multiforme to evaluate immune features and predict prognosis
Authors: Yang Tian , Sun Zhiyou , Li Chujun , Cheng Kexin , Ma Hongwei , Ren Yanhong , Zhang Zhengkui , Yu Rutong 
Published: March 2023

Abstract
Background: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor in adults. The predictive role of SH2D4A has been shown to be closely related to various cancers progression,but there is no comprehensive analysis of the clinical significance in glioblastoma. Hence, this study aimed to explore the relationship between the prognosis of GBM and SH2D4A expression.
Methods: The expression of SH2D4A in GBM was analyzed using TIMER2.0 and GEPIA, and validated by qRT-PCR experiments. The CGGA database analyzed the differential expression of SH2D4A in glioma and evaluated the impact of SH2D4A on the survival of glioma patients.LinkedOmics database and GeneMANIA database were studied for SH2D4A co-expression network. A lasso regression model and nomogram were constructed to assess the prognosis of GBM. TCGA database was used to do a GSEA to find functional differences. The relationship between SH2D4A expression and tumor-infiltrating immune cells was analyzed using xCELL, the CIBERSORT algorithm and the TIMER database.
Results: In GBM patients, we found that the expression of SH2D4A was upregulated, and the elevated expression of SH2D4A was strongly associated with the grade of the tumor. High SH2D4A expression was found to be a significant independent predictor of poor overall survival (OS) in GBM patients by survival curve analysis and multivariate cox regression analysis. GSEA revealed that SH2D4A was mainly enriched in extracellular matrix tissues, and the expression level of SH2D4A was inversely correlated with the level of infiltration of CD8+T cells, CD4+T cells, B cells, neutrophils and macrophages in GBM, but was positively correlated with the level of dendritic cell infiltration. Immunoassays suggest that altered SH2D4A expression may affect the immune infiltration of GBM tissues and thus affect the survival outcome of GBM.
Conclusion: In addition to being a possible prognostic marker and therapeutic target for GBM, SH2D4A may also accelerate the progression of GBM.
Combined electric and magnetic field therapy enhances bone repair and regeneration in two tibia osteotomy models in sheep.
Authors: Darwiche Salim E. , Kaczmarek Anna , Schwarzenberg Peter , Inglis Brendan J. , Lechmann Beat , Kronen Peter , Ferguson Stephen J. , Dailey Hannah , von Rechenberg Brigitte , Klein Karina 
Published: March 2023

Abstract

Background: Therapies using electromagnetic field technology show evidence of enhanced bone regeneration at the fracture site, potentially preventing delayed or non-unions.
Methods: Combined electric and magnetic field (CEMF) treatment was evaluated in two standardized sheep tibia osteotomy models: a 3 mm non-critical size gap model and a 17 mm critical size defect model augmented with autologous bone grafts, both stabilized with locking compression plates. CEMF treatment was delivered across the fracture gap twice daily for 90 mins, starting 4 days post-operatively (post-OP) until sacrifice (9 or 12 weeks post-OP, respectively). Control groups received no CEMF treatment. Bone healing was evaluated radiographically, morphometrically (micro-CT), biomechanically and histologically.
Results: In the 3 mm gap model, the CEMF group (n=6) exhibited higher callus mineral density compared to the Control group (n=6), two-fold higher biomechanical torsional rigidity and a histologically more advanced callus maturity (no statistically significant differences).
In the 17 mm graft model, differences between the control (n=6) and CEMF group (n=6) were more pronounced. The CEMF group showed a radiologically more advanced callus, a higher callus volume (p=0.003) and a 2.6x higher biomechanical torsional rigidity (p=0.024), combined with a histologically more advanced callus maturity and healing.
Conclusions: This study showed that CEMF therapy notably enhanced bone healing resulting in better new bone structure, callus morphology and superior biomechanical properties. This technology could transform a standard inert orthopedic implant into an active device stimulating bone tissue for accelerated healing and regeneration.
Development and Implementation of the Ontario Dairy Monitoring and Analysis Program
Authors: Kelton David F. , Martin S. Wayne , Hansen Donna S. 
Published: March 2023

The importance of monitoring dairy cattle health and productivity at the herd level has become evident during the last decade (1). To monitor all aspects of the herd's performance, dairy producers and veterinarians need a variety of herd level information based on daily cow and farm events. These data are stored either on the farm or in central databases maintained by agencies serving the dairy industry. Unfortunately, in many cases the information useful for monitoring herd performance has not been gleaned from the existing cow level data, or has not been presented in a fashion that facilitates the monitoring process. The Ontario Dairy Monitoring and Analysis Program (ODMAP) evolved out of a perceived need for a herd level monitoring system that utilized the available herd data and was tailored to the characteristics of the Ontario dairy industry. The objectives of the ODMAP project included the establishment of a herd level database that could be used 1) to produce periodic summary reports useful to producers and veterinarians for monitoring herd performance, and 2) to investigate the herd level associations among measures of herd management, milk production, reproductive performance, animal health, and profitability. This manuscript outlines the development and implementation of the ODMAP and summarizes some of the data collected during the first year of the project.
Spanish and French summaries in PDF.
Efficacy of Enrofloxacin in the Treatment of Bovine Clinical Mastitis
Authors: Pyörälä S. , Hirvonen J. , Pyörälä E. 
Published: March 2023

Fluoroquinolones form a promising family of new bactericidal antimicrobials. Enrofloxacin is the first antimicrobial of this family available to veterinary medicine. Fluoroquinolones share a wide spectrum of acitivity but are most potent against gram-negative bacteria. They have a large volume of distribution and a low binding to plasma proteins, and are active at low concentrations. Compared with many other antimicrobials, they also have the advantage of being active against intracellular pathogens (8). Very few studies have been made of the pharmacokinetics of enrofloxacin in lactating cows. In one report, enrofloxacin was administered intravenously ( IV) or intramuscularly (IM) to three cows (3). After both routes of administration, the concentration of enrofloxacin in milk rapidly exceeded that in plasma. At a dose of 2.5 mg/kg b.w., inhibitory levels against coliform bacteria and most staphylococci(> 0.25 μg/ ml) were obtained in milk for approximately 24 hours. Using the same dose administered subcutaneously (SC), a concentration of 0.06 μg/ml or more was maintained in the serum and tissue of calves for 24 hours (6).
The bactericidal action of fluoroquinolones, their wide distribution in tissue and their activity against intracellular pathogens make them attractive for the treatment of staphylococcal mastitis. Enrofloxacin could solve the problem posed by the lack of a pharmacokinetically suitable antimicrobial for coliform mastitis. There are no published data on the efficacy of fluoroquinolones in the treatment of mastitis, except one preliminary report on norfloxacin in dry cow therapy (7).
Information on Regulation of Reproductive Cyclicity in Cattle
Authors: Wiltbank Milo C. 
Published: March 2023

Reproductive cycles are regulated by hormones. The purpose of this paper is to give an overview of recent information on hormonal regulation of reproductive cycles in order to allow increased understanding of programs that are currently being developed or will be developed for improving reproductive efficiency in cattle.
Figure 1 shows a typical hormonal profile for 4 key reproductive hormones during the estrous cycle of the cow. Progesterone comes from the corpus luteum and increases after the time of ovulation and then decreases at the time of luteolysis. Prostaglandin (PG) F2a comes from the nonpregnant uterus and is the hormone responsible for luteolysis. AI, shown below PGF2a is secreted in distinct pulses near the time ofluteolysis. Estradiol-17B increases early in the cycle and after the time of luteolysis. This increase in estradiol causes estrus behaviour and the LH surge. The LH surge is responsible for ovulation of the preovulatory follicle. Ovulation occurs about 28 h after the initiation of the LH surge.
The remainder of this manuscript will provide more depth on this simplified view of the estrous cycle by summarizing some recent information on: regulation of follicular development, regulation of corpus luteum function, and how this information can be applied to understanding mechanisms involved postpartum anestrus and development of follicular cysts.
Byt i świat w ontologii eleackiej
Authors: Piętka Dariusz 
Published: March 2023

Przedmiotem artykułu są teorie pierwszych ontologów greckich: Ksenofanesa, Parmenidesa i Melissosa na temat bytu, jego jedności i tożsamości, z uwzględnieniem niektórych poglądów Zenona. Naczelnym problemem artykułu jest pytanie o naturę relacji bytu względem świata u filozofów eleackich. Celem jest opis sposobu rozumienia tego powiązania, zaproponowanego przez każdego z nich. W rezultacie analiz porównawczych, opierając się na badaniu zachowanych fragmentów tekstów starożytnych, okazało się, że Ksenofanes jest autorem koncepcji Jedno-Boga tożsamego ze światem, zaś Parmenides sformułował oryginalną teorię bytu transcendującego świat cielesny, ale stanowiącego jego rację tożsamości jako całości. Przedstawiona w artykule interpretacja poglądów Parmenidesa stanowi ujęcie odmienne od powszechnie przyjmowanego. Melissos natomiast wykorzystując Parmenidejski sposób rozumienia bytu jako czegoś jednego umieszcza go w świecie. Zbliża to poglądy Melissosa do poglądów Ksenofanesa. Każda z tych teorii nastręcza jednak wielu problemów interpretacyjnych, wynikających przede wszystkim z niewielkiej ilości tekstów źródłowych.
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Zgłoszono: 06/11/2022. Zrecenzowano: 13/12/2022. Zaakceptowano do publikacji: 20/12/2022
In the Quest for Natural Living: the Taoist and Jungian Roots of Arnold Mindell’s Therapeutic Path
Authors: Kowalski Arian 
Published: March 2023

In this article, I would like to take a closer look at the philosophical meaning of the term “process,” which is a fundamental category in Arnold Mindell’s psychology. The Taoist origins of this concept go back to the Tao – the principle of the universe. Tao is the process of passing into each other the opposite aspects of the monastically understood Qi energy. Mindell was also inspired by the analytical psychology of Carl Gustav Jung, which emphasizes the importance of archetypal, unconscious mental processes and their impact on the ego through random thoughts, fantasies or dreams. Mindell distinguishes between a primary process (mental content that we are aware of) and a secondary process (mental content that we are not aware of) to explain what connects a person with her natural living – an unadulterated existence through rigid boundaries between the two processes acquired during one’s upbringing. The purpose of this article is to explore the Jungian and Taoist roots of Mindell’s psychology and to characterize individual lives as confronting their entire processual being, thus becoming natural living.
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Received: 14/04/2022. Reviewed: 19/10/2022. Accepted: 24/10/2022.
Treatment of anterior open bite and an ankylosed incisor by applying multiloop edgewise archwire, mini-implants, and dentoalveolar distraction
Authors: Kim Tae-Woo , Hwang Soon Jung , Wu Tingxi 
Published: March 2023

ABSTRACT
An 18.7-year-old female patient with an anterior open bite and an ankylosed left maxillary central incisor was referred from a private orthodontic clinic. Canine relationships were Class II and molar relationships were Class I. The open bite was closed with the multiloop edgewise archwire and up-and-down elastics. The maxillary left central incisor was extruded by dentoalveolar distraction assisted with mini-implants. Active treatment took 2 years and 1 month, and the treatment result remained stable 14 months after debonding.
Desain dan analisis chassis tipe tubular space frame
Authors: Kahfi M. Wildanul , Mufarida Nely Ana , Kosjoko Kosjoko 
Published: March 2023

Automotive is one of the mainstay products in the world today, especially in 4-wheeled vehicles and 2-wheeled vehicles. One of them is electric cars whose main driving force uses electric power. Some of the advantages possessed by electric vehicles with oil-fueled vehicles include free air police from exhaust fumes, no odor and a smoother motor sound. The development of electric cars in Indonesia, which is now growing rapidly, especially among university students, is being held with competitions to make electric cars that are worthy of being marketed. For that we need an energy-efficient car chassis design that is in accordance with the regulations of the Indonesian Electric Car Competition (KMLI). Research on the design chassis in previous studies has been done with different dimensions, materials, and types of chassis used. The purpose of this research is to design and analyze chassis type Tubular Space Frame using Solidworks 2019 Software in the hope of producing a chassis that is strong, lightweight and has flexibility properties while still paying attention to the Factor of Safety (FOS) when used. The chassis designed using Solidwork 2019 software has dimensions, including: Length = 1883.40 mm, Height = 1033.40 mm, Width = 721.34 mm. The results of the analysis and determination of the material between Aluminum 6061-T6 and Aluminum 6063-T6 used with a comparison of the values ​​of stress, displacement, and safety of factors that occur in the frame, it can be concluded that the chassis is a tubular type with the use of pipes 34.4 x 26.64 3.38 mm thick and 21.36 x 15.50 mm thick 2.77 mm is the most secure chassis and the weight is quite light.