bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Interosseous Tendon Inflammation in the Hand With Different Early Arthritides in a 10‐Year Magnetic Resonance Imaging Study
Authors: van Dijk Bastiaan T. , Reijnierse Monique , van der Helm‐van Mil Annette H. M. 
Published: June 2023

ObjectiveInflammation around the tendons of the hand interosseous muscles (interosseous tendon inflammation [ITI]) was recently identified on magnetic resonance imaging (MRI) in a set of patients with rheumatoid arthritis (RA) and arthralgia. We conducted a large MRI study to assess the prevalence of ITI at diagnosis of RA and of other arthritides, as well as its relationship with clinical signs.MethodsA total of 1,205 patients presenting with various types of early arthritis between 2010 and 2020 underwent contrast‐enhanced hand MRI as part of the prospective Leiden Early Arthritis Cohort. MRI was evaluated with blinding for clinical data, for ITI lateral of metacarpophalangeal (MCP) joints 2–5, and for synovitis/tenosynovitis/osteitis. We assessed ITI presence at baseline per diagnosis and its relationship with clinical characteristics (ie, presence of hand arthritis, increased acute phase reactants, and local joint swelling and tenderness). Logistic regression and generalized estimating equations were used with adjustment for age and established local inflammation features (synovitis/tenosynovitis/osteitis).ResultsA total of 36% of patients with early RA (n = 532) had ITI; this was similar in patients with anti–citrullinated protein antibody (ACPA)‐negative RA (37%) and those with ACPA‐positive RA (34%; P = 0.53). ITI occurred regularly in remitting seronegative symmetrical synovitis with pitting edema (60%) and connective tissue diseases (44%) and less frequently in undifferentiated arthritis (14%), psoriatic arthritis (14%), inflammatory osteoarthritis (8%), reactive arthritis (7%), crystal arthritis (7%), and peripheral spondylarthritis (4%). ITI occurred more often in diagnoses with frequent arthritis of the hands (P < 0.001) and increased acute‐phase reactants (P < 0.001). Within RA, ITI occurred together with local MCP joint synovitis (odds ratio [OR] 2.4, 95% confidence interval [95% CI] 1.7–3.4), tenosynovitis (OR 2.4, 95% CI 1.8–3.3), and osteitis (OR 2.2, 95% CI 1.6–3.1) on MRI. Moreover, ITI presence was associated with local MCP joint tenderness (OR 1.6, 95% CI 1.2–2.1) and swelling (OR 1.8, 95% CI 1.3–2.6), independent of age and MRI‐detected synovitis/tenosynovitis/osteitis.ConclusionITI occurs regularly in RA and other arthritides with preferential involvement of hand joints and increased acute‐phase reactants. At the MCP joint level, ITI associates independently with joint tenderness and swelling. Hence, ITI is a newly identified inflamed tissue mainly found in arthritides with particularly extensive and symptomatic inflammation.
Cyanobacteria mitigation using low power ultrasound for gas vesicle collapse
Authors: Bohrerova Zuzana , Yousuf Yousuf , Crafton‐Nelson Elizabeth , Cheng Chin‐Min , Weaver Christopher R. , Weavers Linda K. 
Published: June 2023

AbstractThe ability of a low‐power ultrasound system to alter the physiology of cyanobacteria cells in controlled field applications was evaluated. Serratia sp., a model bacteria, and cyanobacteria collected from a bloom containing Microcystis sp. and Aphanizomenon sp., were placed in sound transparent containers and exposed to ultrasound in field studies. No observed changes in Serratia sp. and cyanobacteria gas vesicles between unexposed controls and ultrasound exposed samples were found. Cell viability and phycocyanin concentration for both model bacteria and environmental samples were not significantly affected. At the highest acoustic pressure measured (3.5 kPa), 1 m from the transducer, the acoustic pressures were over 100× lower than gas vesicle critical collapse pressures. Moreover, the ultrasound frequencies emitted are significantly lower than gas vesicle resonance frequencies. Therefore, consistent with pressure and frequency measurements, we found no evidence that gas vesicle collapse is the mechanism of ultrasound as a cyanobacteria mitigation strategy.
Construction and application of a new CRISPR/Cas12a system in Stenotrophomonas AGS‐1 from aerobic granular sludge
Authors: Meng Wei , Qiao Kai , Liu Fan , Gao Xu , Hu Xuan , Liu Jia , Gao Yiyun , Zhu Jianrong 
Published: June 2023

AbstractAerobic granular sludge (AGS) is a microbial aggregate with a biofilm structure. Thus, investigating AGS in the aspect of biofilm and microbial attachment at the genetic level would help to reveal the mechanism of granule biofilm formation. In this work, a two‐plasmid clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated proteins (Cas)12a genome editing system was constructed to identify attachment genes for the first time in Stenotrophomonas AGS‐1 from AGS. One plasmid contained a Cas12a cassette driven by an arabinose‐inducible promoter, and another contained the specific crRNA and homologous arms (HAs). Acidaminococcus sp. Cas12a (AsCas12a) was adopted and proven to have mild toxicity (compared to Cas9) and strong cleavage activity for AGS‐1. CRISPR/Cas12a‐mediated rmlA knockout decreased attachment ability by 38.26%. Overexpression of rmlA in AGS‐1 resulted in an increase of 30.33% in attachment ability. These results showed that the modulation of rmlA was an important factor for the biofilm formation of AGS‐1. Moreover, two other genes (xanB and rpfF) were knocked out by CRISPR/Cas12a and identified as attachment‐related genes in AGS‐1. Also, this system could achieve point mutations. These data indicated that the CRISPR/Cas12a system could be an effective molecular platform for attachment gene function identification, which would be useful for the development of AGS in wastewater treatment.
Increased yield of 2‐O‐α‐d‐glucopyranosyl‐l‐ascorbic acid synthesis by α‐glucosidase using rational design that regulating the ground state of enzyme and substrate complex
Authors: Zheng Shao‐Yan , Zhou Wei‐Jie , Lin Xiang‐Na , Li Fei‐Fei , Xie Chun‐Fang , Liu Da‐Ling , Yao Dong‐Sheng 
Published: June 2023

AbstractBackgroundα‐Glucosidase (AG) is a bifunctional enzyme, it has a capacity to synthesize 2‐O‐α‐d‐glucopyranosyl‐l‐ascorbic acid (AA‐2G) from l‐ascorbic acid (L‐AA) and low‐cost maltose under mild conditions, but it can also hydrolyze AA‐2G, which leads to low synthesis efficiency of AA‐2G.Main Methods and Major ResultsThis study introduces a rational molecular design strategy to regulate enzymatic reactions based on inhibiting the formation of ground state of enzyme‐substrate complex. Y215 was analyzed as the key amino acid site affecting the affinity of AG to AA‐2G and L‐AA. For the purpose of reducing the hydrolysis efficiency of AA‐2G, the mutant Y215W was obtained by analyzing the molecular docking binding energy and hydrogen bond formation between AG and the substrates. Compared with the wild‐type, isothermal titration calorimetry (ITC) results showed that the equilibrium dissociation constant (KD) of the mutant for AA‐2G was doubled; the Michaelis constant (Km) for AA‐2G was reduced by 1.15 times; and the yield of synthetic AA‐2G was increased by 39%.Conclusions and ImplicationsOur work also provides a new reference strategy for the molecular modification of multifunctional enzymes and other enzymes in cascade reactions system.
Circadian rhythm of daytime sleepiness in pediatric narcolepsy: A pilot study
Authors: Ouyang Hui , Zhou Zechen , Dai Xiaotong , Zhang Jun 
Published: June 2023

AbstractBackground and objectiveExcessive daytime sleepiness (EDS) has been far back reported as the most disabling symptom in the pediatric narcoleptic patients. However, there is a lack of studies to examine the circadian rhythms of EDS in pediatric narcoleptic population. Therefore, we aim to investigate the circadian rhythm of EDS in pediatric narcolepsy patients.MethodsWe identified 50 pediatric narcoleptic patients (36 males and 14 females, mean age 13.68 ± 2.75 years). Data were collected through interviews and the relevant questionnaires (children depression inventory [CDI] and the pediatric quality of life inventory [PedsQL]).ResultThe frequencies of sleep attacks during different intervals of the day differed significantly, with higher frequency in the morning (p < .001). The times of sleep attacks in the morning and in the afternoon were significantly associated with the degree of impairment on class and the severity of worry about sleepiness, with spearman correlation coefficient ranging from .289 to .496 (p < .05). The total scores of PedsQL and CDI differed significantly among morning sleepiness dominant, afternoon sleepiness dominant, and evening sleepiness dominant groups (p = .042, p = .040). The severity scores of the narcoleptic patients’ sleepiness had two peaks, one of which occurred at 16:00, and the other peaks occurred at about 11:00.ConclusionThese results suggest that changes based on the circadian rhythm of sleepiness of the pediatric narcoleptic patients should be made in the treatment strategy. In addition, regulating the secretion of melatonin could serve as a promising treatment to relieve sleepiness in the future.
The impact of stimulus configuration on visual short‐term memory decline in normal aging and mild cognitive impairment
Authors: Sapkota Raju P. , van der Linde Ian , Grunwald Iris Q. , Upadhyaya Tirthalal , Lamichhane Nirmal , Pardhan Shahina 
Published: June 2023

AbstractIntroductionWhen we memorize simultaneous items, we not only store information about specific items and/or their locations but also how items are related to each other. Such relational information can be parsed into spatial (spatial configuration) and identity (object configuration) components. Both these configurations are found to support performance during a visual short‐term memory (VSTM) task in young adults. How the VSTM performance of older adults is influenced by object/spatial configuration is less understood, which this study investigated.MethodsTwenty‐nine young adults, 29 normally aging older adults, and 20 older adults with mild cognitive impairment (MCI) completed two yes–no memory‐recognition experiments for four simultaneously presented items (2.5 s). Test display items were presented either at the same locations as the memory items (Experiment 1) or were globally shifted (Experiment 2). One of the test display items (target) was highlighted with a square box; participants indicated whether this item was shown in the preceding memory display. Both experiments comprised four conditions where nontarget items changed as follows: (i) nontarget items remained the same; (ii) nontarget items were replaced by new items; (iii) nontarget items switched locations; (iv) nontarget items were replaced by square boxes.ResultsPerformance (% correct) in both older groups was significantly reduced than young adults in both experiments and each condition. For the MCI adults, significantly reduced performance (vs. normal older adults) was found only for Experiment 1.ConclusionVSTM for simultaneous items declines significantly in normal aging; the decline is not influenced differently by spatial/object configuration change. The ability of VSTM to differentiate MCI from normal cognitive aging is apparent only where the spatial configuration of stimuli is retained at original locations. Findings are discussed in terms of the reduced ability to inhibit irrelevant items and location priming (by repetition) deficits.
Inhibition of FGFR3 upregulates MHC‐I and PD‐L1 via TLR3/NF‐kB pathway in muscle‐invasive bladder cancer
Authors: Wu WenBo , Chen Lei , Jia GaoZhen , Tang QiLin , Han BangMin , Xia ShuJie , Jiang Qi , Liu HaiTao 
Published: June 2023

AbstractBackgroundImproving the potency of immune response is paramount among issues concerning immunotherapy of muscle‐invasive bladder cancer (MIBC).MethodsOn the basis of immune subtypes, we investigated possible molecular mechanisms involved in tumor immune escape in MIBC. According to the 312 immune‐related genes, three MIBC immune subtypes were clustered.ResultsCluster 2 subtype is characterized by FGFR3 mutations and has a better clinical prognosis. However, the expression levels of MHC‐I and immune checkpoints genes were the lowest, indicating that this subtype is subject to immune escape and has a low response rate to immunotherapy. Bioinformatics analysis and immunofluorescence staining of clinical samples revealed that the FGFR3 is involved in the immune escape in MIBC. Besides, after FGFR3 knockout with siRNA in RT112 and UMUC14 cells, the TLR3/NF‐kB pathway was significantly activated and was accompanied by upregulation of MHC‐I and PD‐L1 gene expression. Furthermore, the use of TLR3 agonists poly(I:C) can further improve the effect.ConclusionTogether, our results suggest that FGFR3 might involve in immunosuppression by inhibition of NF‐kB pathway in BC. Considering that TLR3 agonists are currently approved for clinical treatment as immunoadjuvants, our study might provide more insights for improving the efficacy of immunotherapy in MIBC.
A rare case of dentinogenic ghost cell tumor with concomitant odontoma
Authors: Okui Tatsuo , Morioka Reon , Iwahashi Teruaki , Matsuda Yuhei , Ishizuka Shinji , Okuma Satoe , Tatsumi Hiroto , Kanno Takahiro 
Published: June 2023

Key Clinical MessageA case of dentinogenic ghost cell tumor occurring simultaneously with a clinically diagnosed odontoma. The occurrence of epithelial and mesenchymal tumors at the same site is very rare, but should be kept in mind during pathological diagnosis.AbstractDentinogenic ghost cell tumor (DGCT) is a rare and benign odontogenic tumor composed of ghost cells, calcified tissue, and dentin. We present an extremely rare case of a 32‐year‐old female who was clinically diagnosed with an odontoma presenting with a painless swelling in her maxilla. Radiographic examination showed a well‐defined radiolucent lesion with tooth‐like calcified areas. The tumor was resected under general anesthesia. No recurrence was noted at the 12‐month follow‐up. Histopathological examination of the surgically resected tumor yielded a diagnosis of DGCT with odontoma.
Pathologic complete response to neoadjuvant imatinib of a gastric stromal tumor with concomitant mutations in KIT: A case report and literature review
Authors: Rekaya Mariem Ben , Ksontini Feryel , Kacem Linda Bel Haj , Sassi Farah , Harigua‐Souiai Emna , Boujneh Ryma , H'mayada Ahmed , Zaimi Yosra , Ayadi Mouna , Trabelsi Mediha , Mrad Ridha , Rammeh Soumaya 
Published: June 2023

Key clinical messageWe report the first case of pathologic complete response (pCR) to neoadjuvant imatinib in a gastric stromal tumor harboring KIT mutations in both exons 11 and 9. The significance of this co‐occurrence is unknown and might increase the responsiveness of gastrointestinal stromal tumors (GISTs) to imatinib.AbstractpCR of GIST to neoadjuvant imatinib is rare. We report a case of pCR to neoadjuvant imatinib in a gastric stromal tumor that harbored co‐occurrence of multiple KIT mutations in exons 11 and 9. This co‐occurrence in exons 9 and 11 is the first to be reported in the English literature.
Peri‐implant proximal femur fracture in a poliomyelitis survivor: A surgical and medical challenge
Authors: Sakellariou Evangelos , Galanis Athanasios , Vavourakis Michail , Papagrigorakis Eftychios , Vlachos Christos , Zachariou Dimitrios , Vasiliadis Elias , Pneumaticos Spiros 
Published: June 2023

Key Clinical MessageThe treatment of long bone fractures in post‐polio survivors is indubitably an exacting task. Out of this complicated case presented in this paper, it can be deduced that it is attainable to repair a peri‐implant subtrochanteric refracture or a complex non‐union of the proximal femur with plate and screws with grafting.AbstractPost‐polio survivors are prone to low‐energy bone fractures. The management of such cases is exigent, as no literature data indicate the best surgical approach. This paper presents an intricate peri‐implant proximal femoral fracture in a polio survivor treated in our institution and accentuates the various challenges we encountered.
Steroid‐induced inflammatory papillary hyperplasia?—A new entity in a non‐denture wearing patient
Authors: Gupta Abhishek , Lamichhane Ram Sudan , Redhu Anju 
Published: June 2023

Key Clinical MessageInflammatory papillary hyperplasia can be seen in a non‐denture‐wearing patient also therfore other etiologies need to be explored as well.AbstractInflammatory papillary hyperplasia (IPH) is a benign lesion of the palatal mucosa, usually found in denture wearers. This case history report describes an example of the dentate patients with no history of wearing maxillary prostheses and highlights the importance of professional awareness to diagnose IPH among non‐denture‐wearing patients.
Electrochemical Flow Reactor Design Allows Tunable Mass Transport Conditions for Operando Surface Enhanced Infrared Absorption Spectroscopy
Authors: Avilés Acosta Jaime E. , Lin John C. , Un Lee Dong , Jaramillo Thomas F. , Hahn Christopher 
Published: June 2023

AbstractIn situ attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR‐SEIRAS) is often used to investigate the near‐surface electrocatalytic reaction environment. However, there is a gap in directly correlating the near‐surface reaction environment with electrocatalytic reaction rates. To that end, we designed an electrochemical flow reactor for operando electrochemical ATR‐SEIRAS and demonstrate its capability with the CO2 reduction reaction (CO2RR). Roughened gold catalyst thin films are prepared on ATR silicon crystals as a model system to probe local species under CO2RR conditions in 0.1 M KHCO3. We measured changes in the interfacial CO2 concentration as a function of applied potential and electrolyte flow rate in operando, allowing us to correlate the changes in reaction rates with the observed CO2 concentration. Including the choice of the catalyst and electrolyte, coupling hydrodynamic control with ATR‐SEIRAS in this platform enables investigations of how the local microenvironment affects the activity and selectivity of electrochemical reactions.
Phenomenological understanding of Black counselor education students at Predominately White Institutions, Historically Black Colleges and Universities, and Hispanic Serving Institutions
Authors: Brooks Michael , Whittaker Tyra Turner , Wang Yudan , Adams Justin , Matthews Tazeur 
Published: June 2023

AbstractThe experiences of Black counseling students at Predominantly White Institutions are well documented. Using a transcendental phenomenological environmental research design, we explored how 12 Black counselor education graduates experienced their respective institutions across three types of academic institutions—Predominantly White Institutions, Historically Black Colleges and Universities, and Hispanic Serving Institutions. Three salient themes emerged—(1) exposure, (2) real‐life exposure, and (3) cultural oasis. The researchers also discussed implications for counselor education programs and future research.
Computational fluid dynamics‐based virtual angiograms for the detection of flow stagnation in intracranial aneurysms
Authors: Hadad Sara , Karnam Yogesh , Mut Fernando , Lohner Rainald , Robertson Anne M. , Kaneko Naoki , Cebral Juan R. 
Published: June 2023

AbstractThe goal of this study was to test if CFD‐based virtual angiograms could be used to automatically discriminate between intracranial aneurysms (IAs) with and without flow stagnation. Time density curves (TDC) were extracted from patient digital subtraction angiography (DSA) image sequences by computing the average gray level intensity inside the aneurysm region and used to define injection profiles for each subject. Subject‐specific 3D models were reconstructed from 3D rotational angiography (3DRA) and computational fluid dynamics (CFD) simulations were performed to simulate the blood flow inside IAs. Transport equations were solved numerically to simulate the dynamics of contrast injection into the parent arteries and IAs and then the contrast retention time (RET) was calculated. The importance of gravitational pooling of contrast agent within the aneurysm was evaluated by modeling contrast agent and blood as a mixture of two fluids with different densities and viscosities. Virtual angiograms can reproduce DSA sequences if the correct injection profile is used. RET can identify aneurysms with significant flow stagnation even when the injection profile is not known. Using a small sample of 14 IAs of which seven were previously classified as having flow stagnation, it was found that a threshold RET value of 0.46 s can successfully identify flow stagnation. CFD‐based prediction of stagnation was in more than 90% agreement with independent visual DSA assessment of stagnation in a second sample of 34 IAs. While gravitational pooling prolonged contrast retention time it did not affect the predictive capabilities of RET. CFD‐based virtual angiograms can detect flow stagnation in IAs and can be used to automatically identify aneurysms with flow stagnation even without including gravitational effects on contrast agents.
Complete coverage planning with clustering method for autonomous mobile robots
Authors: Aydemir Hamza , Tekerek Mehmet , Gök Mehmet 
Published: June 2023

SummaryComplete coverage planning (CCP) is a task to cover the entire area on the map, according to the job description of the autonomous mobile robot. The most widely used method for CCP in the literature is the grid‐based coverage method. In this method, the problem is processing the partially filled cell as completely filled, which reduces the coverage performance. The ability to use the clustering method, which will be created by considering the characteristics of the environment, was determined as a research question to solve this problem. In this direction, it is aimed to use K‐means++ algorithm, which is a widely used clustering algorithm and segmentation technique. In this context, an offline K‐means++ complete coverage planning (Km++CCP) method, in which the navigable area on the map of the indoor where a mobile robot will navigate is clustered using the K‐means++ algorithm and the centroids can be used as waypoints, is proposed. To test the proposed method, 2 simulations and 36 real‐world experiments were conducted. The indoor coverage ratio of Km++CCP was calculated higher than the grid‐based method in all experiments.