bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating TCGA and single-cell sequencing data for colorectal cancer: a 10-gene prognostic risk assessment model
Authors: Lu Di , Li Xiaofang , Yuan Yuan , Li Yaqi , Wang Jiannan , Zhang Qian , Yang Zhiyu , Gao Shanjun , Zhang Xiulei , Zhou Bingxi 
Published: September 2023

AbstractColorectal cancer represents a significant health threat, yet a standardized method for early clinical assessment and prognosis remains elusive. This study sought to address this gap by using the Seurat package to analyze a single-cell sequencing dataset (GSE178318) of colorectal cancer, thereby identifying distinctive marker genes characterizing various cell subpopulations. Through CIBERSORT analysis of colorectal cancer data within The Cancer Genome Atlas (TCGA) database, significant differences existed in both cell subpopulations and prognostic values. Employing WGCNA, we pinpointed modules exhibiting strong correlations with these subpopulations, subsequently utilizing the survival package coxph to isolate genes within these modules. Further stratification of TCGA dataset based on these selected genes brought to light notable variations between subtypes. The prognostic relevance of these differentially expressed genes was rigorously assessed through survival analysis, with LASSO regression employed for modeling prognostic factors. Our resulting model, anchored by a 10-gene signature originating from these differentially expressed genes and LASSO regression, proved adept at accurately predicting clinical prognoses, even when tested against external datasets. Specifically, natural killer cells from the C7 subpopulation were found to bear significant associations with colorectal cancer survival and prognosis, as observed within the TCGA database. These findings underscore the promise of an integrated 10-gene signature prognostic risk assessment model, harmonizing single-cell sequencing insights with TCGA data, for effectively estimating the risk associated with colorectal cancer.
Simultaneous Delignification and Furfural Production of Palm Oil Empty Fruit Bunch by Novel Ternary Deep Eutectic Solvent
Authors: Muryanto Muryanto , Sudiyani Yanni , Darmawan Muhammad Arif , Handayani Eka Mardika , Gozan Misri 
Published: September 2023

AbstractThe most considerable solid waste from crude palm oil plants is oil palm empty fruit bunch (OPEFB) which contains cellulose, lignin, and hemicellulose. Hemicellulose can be hydrolyzed to xylose and then converted to furfural via dehydration. Pretreatment is one of the steps in the bioconversion of lignocellulose material to reduce lignin. This study developed a one-pot process to conduct pretreatment and furfural production simultaneously. This process uses a green solvent called ternary deep eutectic solvent (DES). DES was synthesized by mixing choline chloride, oxalic acid, and ethylene glycol with a molar ratio of 1:1:2 (CHOAEG). Simultaneous delignification and furfural production were carried out in a stainless steel reactor. The temperature was varied at 100, 120, and 150 °C, with the various processing time at 30, 60, and 90 min, respectively. The highest furfural concentration reached 9.68 g/L, and the delignification was achieved up to 55.81% at 150 °C for 90 min. The OPEFB pretreated was hydrolyzed by cellulase and achieved 90.79% glucose yield. Overall, the simultaneous delignification and furfural production process by ternary DES CHOAEG demonstrated a novel and efficient process by reducing the number of complex processes stages of biorefinery lignocellulose.
A new bio-based zwitterionic surfactant with improved interfacial activity by optimizing hydrophilic head
Authors: Wang Wei , Liang Ming-Yue , Lang Jian-Qiao , Mtui Homely Isaya , Yang Shi-Zhong , Mu Bo-Zhong 
Published: September 2023

AbstractBio-based zwitterionic surfactant attracts increasing attention to its application potential of surfactant flooding for enhanced oil recovery because of its environmental friendliness, renewability, and stable interfacial performance under high salinity and high temperature. However, bio-based zwitterionic surfactants which can be applied at the temperature higher than 100°C are still rare. In this paper, a new surfactant, phenyloleamide ethyl hydroxysulfonyl quaternary ammonium salt (PEHSQA), was obtained from biomaterial methyl oleate through alkylated, amidated, and quaternized reactions. As N, N-dimethyl propanediamine (a common amidated reagent in the synthesis of bio-based zwitterionic surfactants derived from fatty acids and esters) has one more carbon atom than N,N-dimethyl-1,2-ethanediamine, the latter was adopted as the amidated reagent optimizing the hydrophilic head of surfactant to improve the interfacial activity, especially the thermal resistance. The interfacial tension (IFT) between Daqing oil and stratum water could be maintained at 2.13×10−3 mN/m with the aid of 1 g/L PEHSQA. Furthermore, the IFT could be maintained at the level of 10−3 mN/m under the condition of 120 °C, with added NaCl of 20 g/L or Ca2+ of 2500 mg/L respectively. The optimization of reducing carbon number in the hydrophilic head of the bio-based zwitterionic surfactant can make a better balance between hydrophilicity and hydrophobicity offered by long fatty carbon chain, which can improve the interfacial activity especially the thermal resistance effectively.
Optimized solubility and bioavailability of genistein based on cocrystal engineering
Authors: Wang Zhipeng , Li Qi , An Qi , Gong Lixiang , Yang Shiying , Zhang Baoxi , Su Bin , Yang Dezhi , Zhang Li , Lu Yang , Du Guanhua 
Published: September 2023

AbstractWith various potential health-promoting bioactivities, genistein has great prospects in treatment of a series of complex diseases and metabolic syndromes such as cancer, diabetes, cardiovascular diseases, menopausal symptoms and so on. However, poor solubility and unsatisfactory bioavailability seriously limits its clinical application and market development. To optimize the solubility and bioavailability of genistein, the cocrystal of genistein and piperazine was prepared by grinding assisted with solvent based on the concept of cocrystal engineering. Using a series of analytical techniques including single-crystal X-ray diffraction, powder X-ray diffraction, Fourier transform infrared spectroscopy, differential scanning calorimetry and thermogravimetric analysis, the cocrystal was characterized and confirmed. Then, structure analysis on the basis of theoretical calculation and a series of evaluation on the stability, dissolution and bioavailability were carried out. The results indicated that the cocrystal of genistein and piperazine improved the solubility and bioavailability of genistein. Compared with the previous studies on the cocrystal of genistein, this is a systematic and comprehensive investigation from the aspects of preparation, characterization, structural analysis, stability, solubility and bioavailability evaluation. As a simple, efficient and green approach, cocrystal engineering can pave a new path to optimize the pharmaceutical properties of natural products for successful drug formulation and delivery.
Graphical Abstract
Tree-ring dating of the common trees in Wadi Allaqi Biosphere Reserve, South-East Egypt
Authors: Salem Ashraf , Shedded Mohamed , Al-Sodany Yassin , Shaltout Kamal 
Published: September 2023

AbstractTwenty-five increment cores and tree discs were sampled for four common trees in Wadi Allaqi, an extremely arid region in South-East Egypt (19 for Acacia tortilis subsp. raddiana (Savi) Brenan and 2 for each of Balanites aegyptiaca (L.) Delile, Acacia ehrenbergiana Hayne, and Tamarix nilotica (Ehrenb.) Bunge). The main aim of the current study is to achieve a longer temporal perspective on growth, longevity, and marginal parenchyma of the wood samples. The growth ring boundaries of the acacias are differentiated by thin parenchyma bands, which run around the entire stem discs. Samples of Acacia tortilis subsp. raddiana were located along this Wadi from its upstream to downstream parts; based on the mean distance between the bands of marginal parenchyma, longevity, based on the marginal parenchyma bands, indicated that Acacia tortilis subsp. raddiana grew slowly and some of its studied individuals reflected 2 age scenarios in the downstream part, while the measurement interval reflected an established date of around 1884 or 1886. Both scenarios grew fast over a long period of time, and so the chiefly recent growth was dated back to 1885. Approximate dates for the midstream part dated back to 1648, while the overall growth for the upstream part dated back to 1482. Samples of Balanites aegyptiaca may be established between 1608 and 1715, while those of Acacia ehrenbergiana may be established between 1945 and 1975. Tamarix nilotica swiftly established itself, and a new ecosystem replaced the severe arid habitat after the dropping of the water level in 1980s.Two scenarios of age are probably true for the downstream part, implying a date of establishment between 1884 and 1886. It was also discovered that the outdated scenario for B. aegyptiaca and A. ehrenbergiana is more in line with asymptotic value and current growth, indicating larger possibility for future expansion.
Body image impact on quality of life and adolescents’ binge eating: the indirect role of body image coping strategies
Authors: Bianchi Dora , Schinelli Anthony , Fatta Laura Maria , Lonigro Antonia , Lucidi Fabio , Laghi Fiorenzo 
Published: September 2023

Abstract
Purpose
The role of body image in adolescent binge eating is widely confirmed, albeit the various facets of this relationship are still mostly unexplored. Within the multidimensional body image framework, this study hypothesized the indirect effects of three body image coping strategies (positive rational acceptance, appearance fixing, avoidance) in the expected relationship between the perceived impact of body image on individuals’ quality of life and binge eating symptoms.

Methods
Participants were 715 adolescents aged 15–21 years (49.1% girls) recruited in Italian schools. An anonymous self-report online survey was administered. A multiple mediation model was tested.

Results
A more positive perceived impact of body image on quality of life was a negative predictor of adolescents’ binge eating, controlling for individual levels of body satisfaction. Three indirect effects were found in this relationship: on one hand, the positive body image impact reduced binge eating via increasing positive rational acceptance (M1), and via reducing avoidance (M2); on the contrary, the positive body image impact also enhanced binge eating via increasing appearance fixing (M3).

Conclusions
The body image impact on quality of life can be alternatively protective—when adaptive coping is solicited, and maladaptive strategies are reduced—or a risk factor, which may increase binge eating by soliciting appearance fixing.

Level III
Evidence obtained from cohort or case–control analytic studies.
Response of commercial classes of wheat to contrasting irrigation regimes
Authors: Ledesma-Ramírez Lourdes , Solís-Moya Ernesto , Mariscal-Amaro Luis Antonio , Huerta-Espino Julio , Montero-Tavera Victor , Gámez-Vázquez Alfredo Josué , Buenrostro-Rodríguez Juan Francisco , González-Figueroa Sarahyt Santamaria 
Published: September 2023

AbstractA 3-year experiment was established in which four wheat classes were evaluated including soft, medium hard, hard and durum wheat with the objective to determine which class is more efficient in water use under reduced irrigation. The experiments were established during three growing seasons (2016–2018). The amount of water applied were: 26, 34 and 54 cm distributed in 2, 3 and 5 irrigations, respectively. Eighteen genotypes from each wheat class were evaluated in an alpha lattice design with three replicates. Phenological data, yield and yield components were analyzed. Yield in the two-irrigation regime ranged from 3974 to 5436, 4453 to 6909 under three and 6177 to 9107 kg ha−1 under five. Correlation analysis showed that with two irrigations there is a greater association of grain yield with thousand kernel weight (TKW), but under three and five irrigations, grain yield was associated to a greater degree with kernel number per unit area (KNO). When analyzing the grain yield, it was observed that under reduced irrigation (2 and 3 irrigations), bread wheats were superior to durum wheats regardless of the class. Under five irrigations, durum wheats showed the highest yield (8303 kg ha−1); however, they were only significantly superior (Tukey ≤ 0.05) to the hard wheats (7721 kg ha−1). In general, the tested wheats showed higher water efficiency (considered as water productivity) under reduced irrigation than under normal irrigation. The lowest losses in water productivity when going from two to five irrigations were observed in durum wheats (0.17 kg m−3) and the highest losses in the hard wheats (0.38 kg m−3).
Well-being grants in an academic medical center: A case example
Authors: Olson Lauren E. , Bredella Miriam A. , Levy Anne S. , Mehta Darshan H. 
Published: September 2023

Abstract
Academic medical centers (AMCs) rely on engaged and motivated faculty for their success. Significant burnout among clinical and research faculty has resulted in career disengagement and turnover. As such, AMCs must be vested in cultivating faculty engagement and well-being through novel initiatives that support faculty. The Well-Being Education Grants program was established by the Office for Well-Being within the Center for Faculty Development at Massachusetts General Hospital to provide the impetus many faculty needed to dedicate time to their well-being, demonstrating that investments in multi-component interventions around faculty well-being require resources and funding.
Comparison theorem and stability under perturbation of transition rate matrices for regime-switching processes
Authors: Shao Jinghai 
Published: September 2023

AbstractA comparison theorem for state-dependent regime-switching diffusion processes is established, which enables us to pathwise-control the evolution of the state-dependent switching component simply by Markov chains. Moreover, a sharp estimate on the stability of Markovian regime-switching processes under the perturbation of transition rate matrices is provided. Our approach is based on elaborate constructions of switching processes in the spirit of Skorokhod’s representation theorem varying according to the problem being dealt with. In particular, this method can cope with switching processes in an infinite state space and not necessarily of birth–death type. As an application, some known results on the ergodicity and stability of state-dependent regime-switching processes can be improved.
Therapists’ beliefs about excessive reassurance seeking and helping manage it: does experience play a role?
Authors: Halldorsson Brynjar , Salkovskis Paul M. 
Published: September 2023

Abstract
Excessive reassurance seeking (ERS) is believed to play an important role in maintaining mental health problems, in particular anxiety disorders such as obsessive-compulsive disorder and health anxiety. Despite this, therapists commonly give into patients’ requests for reassurance in clinical settings and are generally unsure how to handle the issue both in therapy itself and concerning advice to the patient’s loved ones. In order to increase our understanding of therapists’ perception of ERS and how interventions for ERS are managed, we examined therapists’ perception and understanding of ERS, including its function, which emotional problems therapists associate it with, and what treatment interventions they consider important for managing ERS. Qualified therapists (n=197) were benchmarked against international expert consensus (n=20) drawn from leading clinical researchers. There was evidence that clinical experience right up to the expert level may result in less reassurance giving within treatment settings. Still, there were enough inconsistencies between the experts and other clinicians to suggest that ERS remains poorly understood and is not consistently dealt with clinically. Results are discussed in terms of how current treatment interventions may be limited for treating ERS, highlighting the need to consider new approaches for dealing with this complicated interpersonal behaviour.

Key learning aims

(1)
To describe the role of excessive reassurance seeking in checking behaviour, including its negative personal and interpersonal consequences.

(2)
To learn that therapists commonly report finding it difficult to manage reassurance seeking.

(3)
To learn that therapists’ beliefs about excessive reassurance seeking may play a key role in helping us understand how to tackle this complicated behaviour.

(4)
To consider what therapeutic interventions may be appropriate and helpful for treating excessive reassurance seeking.
Cognitive therapy for PTSD following birth trauma and baby loss: clinical considerations
Authors: Kerr Alice , Warnock-Parkes Emma , Murray Hannah , Wild Jennifer , Grey Nick , Green Catherine , Clark David M. , Ehlers Anke 
Published: September 2023

Abstract
Post-traumatic stress disorder (PTSD) after traumatic birth can have a debilitating effect on parents already adapting to significant life changes during the post-partum period. Cognitive therapy for PTSD (CT-PTSD) is a highly effective psychological therapy for PTSD which is recommended in the NICE guidelines (National Institute for Health and Care Excellence, 2018) as a first-line intervention for PTSD. In this paper, we provide guidance on how to deliver CT-PTSD for birth-related trauma and baby loss and how to address common cognitive themes.

Key learning aims

(1)
To recognise and understand the development of PTSD following childbirth and baby loss.

(2)
To understand how Ehlers and Clark’s (2000) cognitive model of PTSD can be applied to post-partum PTSD.

(3)
To be able to apply cognitive therapy for PTSD to patients with perinatal PTSD, including traumatic baby loss through miscarriage or birth.

(4)
To discover common personal meanings associated with birth trauma and baby loss and the steps to update them.
Clinician experiences on training and awareness of sexual orientation in NHS Talking Therapies Services for Anxiety and Depression
Authors: Ho Jason Kai Yu , O’Rouke Christopher , Laville Allán , Chellingsworth Marie , Callaghan Patrick 
Published: September 2023

Abstract
Previous research that explored sexual minority service users’ experiences of accessing NHS Talking Therapies for Anxiety and Depression Services highlighted the need for specific sexual orientation training. Inconsistent or lack of training may contribute to disparities in treatment outcomes between sexual minority service users and heterosexual service users. The aim of the study was to explore clinicians’ competencies working with sexual minority service users, their experiences of sexual orientation training, their view of current gaps in training provision, and ways to improve training. Self-reported sexual orientation competency scales and open-ended questions were used to address the aims of the study. Participants (n=83) included Psychological Wellbeing Practitioners (PWPs) and high-intensity CBT therapists (HITs). Responses on competency scales were analysed using Kruskal–Wallis tests and thematic analysis was used to analyse qualitative responses. Participants who identified as 25–29 years old had higher scores on the knowledge scale than 45+-year-olds. Bisexual participants also had higher scores on the knowledge subscale than heterosexual participants. Three over-arching themes were identified: (a) training received on sexual minority issues by Talking Therapies clinicians, (b) clinicians’ experiences of accessing and receiving sexual minority training, and (c) perceived gaps in current sexual minority training and ways to improve training. Findings were linked to previous literature and recommendations to stakeholders are made throughout the Discussion section with the view of improving sexual orientation training.

Key learning aims

(1)
To understand current training provision of sexual orientation training across NHS Talking Therapies courses and services in England.

(2)
To consider clinicians’ experiences of challenges and barriers that may prevent them from accessing or implementing sexual orientation training in clinical practice.

(3)
To understand clinicians’ views of the current gaps in training and ways to improve training provision.

(4)
To make recommendations to NHS Talking Therapies for Anxiety and Depression courses and services in ways to improve training on sexual orientation to better meet the learning needs of clinicians and service users.
Factors associated with low birthweight among late preterm singletons in Japan using pregnancy birth registry data
Authors: Kasuga Yoshifumi , Tanaka Kunio , Akita Keisuke , Tamai Junko , Hamuro Asuka , Fukuma Yuka , Hasegawa Keita , Ikenoue Satoru , Tanaka Mamoru 
Published: September 2023

AbstractLate preterm (LP, born between 34 0/7 and 36 6/7 weeks of gestation) infants may experience several adverse outcomes, similar to those experienced by low birthweight (LBW, birthweight <2500 g) infants. However, while LP infants are often born with LBW, the association between LP and LBW remains unknown. This study aimed to investigate LBW rate and independent risk factors for LBW in LP singleton neonates. We retrospectively analyzed data of LP singleton neonates, born between 2013 and 2017, from the Japan Society of Obstetrics and Gynecology Successive Pregnancy Birth Registry System. The exclusion criteria included stillbirths and infants with missing data. Logistic regression analyses were performed to investigate maternal and perinatal factors associated with LBW in LP singletons. LBW was observed in 62.5% (n = 35,113) of 56,160 LP singleton births. In the multiple logistic regression analysis, LBW in LP neonates was independently associated with modifiable maternal factors, including pre-pregnancy underweight, inadequate gestational weight gain, and smoking during pregnancy, as well as non-modifiable factors, including younger maternal age, nulliparity, hypertensive disorder of pregnancy, preeclampsia, cesarean section delivery, and female offspring. According to the Japanese pregnancy birth registry data, more than half of LP neonates were LBW. We previously discussed the issue of LBW regarding infants with different backgrounds, as there are many different causes of LBW. Several risk factors should be subdivided and considered for the risk of LP and LBW.