bluuebunny/crossref_metadata_2025_split huggingface dataset:
Legume‐wide comparative analysis of pod shatter locus PDH1 reveals phaseoloid specificity, high cowpea expression, and stress responsive genomic context
Authors: Marsh Jacob I , Nestor Benjamin J , Petereit Jakob , Tay Fernandez Cassandria G , Bayer Philipp E , Batley Jacqueline , Edwards David 
Published: March 2023

SUMMARYPod dehiscence is a major source of yield loss in legumes, which is exacerbated by aridity. Disruptive mutations in “Pod indehiscent 1” (PDH1), a pod sclerenchyma‐specific lignin biosynthesis gene, has been linked to significant reductions in dehiscence in several legume species. We compared syntenic PDH1 regions across 12 legumes and two outgroups to uncover key historical evolutionary trends at this important locus. Our results clarified the extent to which PDH1 orthologs are present in legumes, showing the typical genomic context surrounding PDH1 has only arisen relatively recently in certain phaseoloid species (Vigna, Phaseolus, Glycine). The notable absence of PDH1 in Cajanus cajan may be a major contributor to its indehiscent phenotype compared with other phaseoloids. In addition, we identified a novel PDH1 ortholog in Vigna angularis and detected remarkable increases in PDH1 transcript abundance during Vigna unguiculata pod development. Investigation of the shared genomic context of PDH1 revealed it lies in a hotspot of transcription factors and signaling gene families that respond to abscisic acid and drought stress, which we hypothesize may be an additional factor influencing expression of PDH1 under specific environmental conditions. Our findings provide key insights into the evolutionary history of PDH1 and lay the foundation for optimizing the pod dehiscence role of PDH1 in major and understudied legume species.
Transfusion of ABO‐group identical red blood cells following uncrossmatched transfusion does not lead to higher mortality in civilian trauma patients
Authors: Yazer Mark H. , Dunbar Nancy M. , Hess John R. , Tuott Erin E. , Bahmanyar Mohammad , Campbell Jessica , Fontaine Magali , Ko Ara , Mi Jian , Murphy Michael F. , Poisson Jessica , Raval Jay S. , Shih Andrew W. , Sperry Jason L. , Staves Julie , Wong Michelle , Yan Matthew T. S. , Ziman Alyssa , Seheult Jansen N. 
Published: March 2023

AbstractBackgroundQuestions persist about the safety of switching non‐group O recipients of group O uncrossmatched red blood cells (RBC) or low titer group O whole blood (LTOWB) to ABO‐identical RBCs during their resuscitation.MethodsThe database of an earlier nine‐center study of transfusing incompatible plasma to trauma patients was reanalyzed. The patients were divided into three groups based on 24‐h RBC transfusion: (1) group O patients who received group O RBC/LTOWB units (control group, n = 1203), (2) non‐group O recipients who received only group O units (n = 646), (3) non‐group O recipients who received at least one unit of group O and non‐group O units (n = 562). Fixed marginal effect of receipt of non‐O RBC units on 6‐ and 24‐h and 30‐day mortality was calculated.ResultsThe non‐O patients who received only group O RBCs received fewer RBC/LTOWB units and had slightly but significantly lower injury severity score compared to control group; non‐group O patients who received both group O and non‐O units received significantly more RBC/LTOWB units and had a slightly but significantly higher injury severity score compared to control group. In the multivariate analysis, the non‐O patients who received only group O RBCs had significantly higher mortality at 6‐h compared to the controls; the non‐group O recipients of O and non‐O RBCs did not demonstrate higher mortality. At 24‐h and 30‐days, there were no differences in survival between the groups.ConclusionProviding non‐group O RBCs to non‐group O trauma patients who also received group O RBC units is not associated with higher mortality.
A Systemic Functional Linguistic Analysis of Clauses Relationship in Luke Gospel Text, Janji Baru Using Kupang Malay
Authors: Ngongo Magdalena , Saputra Nanda , Lasino Lasino , Bahodirovna Akhmedova Mehrinigor 
Published: March 2023

This paper reports on a functional linguistics study of clauses relationship in which it is included as textual meta function of meaning. This textual metafunction is on the level of lexicogrammar. Semantic sequence of figures is realized by a clause complexes series. This paper focused on relationship types between clauses, interdependency and logico-semantic relationship. Data were taken from A Bible, New Testament using Kupang Malay called Janji Baru, especially in Luke of Gospel text. The analysis revealed that parataxis used in text covered coordinate conjunctions such as, deng ’and’, ma, ‘but’, jadi/ko ‘so, for’, ais ju ‘then’. The hypotaxis used subordinate conjunctions, such as, kalo ‘if’’ tagal ‘because/since’, te ‘cause’. The use of parataxis and hypotaxis indicates that semantic sequence is coherence in which conjunctions used in text are usually repeated. It is the same as the use of logico-semantic, such as expansion and projection. The expansion consisted of elaboration, extension, and enhancement. The projection consisted of locution and idea that related to phenomena of a higher order of experience. The idea used verbal process, such as, kastau bilang ‘tell/say’, manyao bilang, ’answer’, tanya bilang ‘ask’, etc. This fact indicates that the idea stated was stressed by using serial verbs or usually a verb was ended by other verb’ This fact indicates to attract listeners’ attention. The locution used mental process, such as, rasa ‘feel’ ‘inga’ ‘remember’. Thus, it can be stated that the use of interdependency and logico-semantic relation was aimed to keep the semantic sequence and text coherence.
The Cross-Cultural Training Needs of Football Coaches
Authors: Borges Mário , Rosado António , Lobinger Babett , Freitas Francisco , de Oliveira Rita F. 
Published: March 2023

The ability to interact and communicate effectively in different cultural contexts has never been more relevant for football coaches because cultural diversity in football has increased in the past decades. Yet, there is a reduced number of institutions that are currently offering cultural training to coaches, and it is unknown whether coaches feel the need for such training. This study aimed to explore the cross-cultural training needs of 115 football coaches with national and/or international experience (M = 13.4 years; SD = 8.53). To maximise recruitment reach, an online survey with open-ended questions was used followed by a qualitative thematic analysis. There were four master themes: cross-cultural training needs in the global football market; cross-cultural awareness is important in football; football-specific cultural training; and training by migrant coaches, no matter how. Most coaches perceived a benefit from cultural training in preparation for international appointments, but some felt that coaches already had cultural awareness because they manage individuals and adjust easily to different cultures. Preferences were shown for cross-cultural training delivered by migrant coaches and with a football-specific focus. This is the first study to explore the perceptions of football coaches about cross-cultural awareness and training needs and provides insights to providers of coach education.
Social economic factors and the risk of multiple chemical sensitivity in a Danish population-based cross-sectional study: Danish Study of Functional Disorders (DanFunD)
Authors: Ahrendt Bjerregaard Anne , Schovsbo Signe Ulfbeck , Gormsen Lise K , Skovbjerg Sine , Eplov Lene Falgaard , Linneberg Allan , Cedeño-Laurent José G , Jørgensen Torben , Dantoft Thomas Meinertz 
Published: March 2023

ObjectivesMultiple chemical sensitivity (MCS) is a rare multisystem and poly-symptomatic disease characterised by a report of various somatic symptoms attributed to inhalation of volatile chemicals in usually harmless doses. The aim was to explore four selected social factors and the risk of MCS in the general Danish population.DesignA cross-sectional general population-based study.SettingThe Danish Study of Functional Disorders was conducted from 2011 to 2015 which included 9656 participants.ParticipantsA total of 8800 participants were included in analyses after observations with missing data on exposure and/or outcome were excluded. A total of 164 cases fulfilled the questionnaire criteria for MCS. Of the 164 MCS cases, 101 reported no comorbid functional somatic disorder (FSD) and were included in a subgroup analysis. A total of 63 MCS cases fulfilled the criteria for at least one additional FSD, this subgroup was not included in further analysis. The remaining study population without MCS or any FSD were regarded as controls.Outcome measuresWe used adjusted logistic regression to calculate OR and 95% CIs of MCS and MCS without FSD comorbidities for each social variable separately including education, employment, cohabitation and subjective social status.ResultsWe found an increased risk of MCS among the unemployed (OR: 2.95, 95% CI: 1.75 to 4.97), and a twofold increased risk of MCS among individuals with low subjective social status (OR: 2.00, 95% CI: 1.08 to 3.70). At the same time, 4 years or more of vocational training were protective of MCS. No significant associations were observed among MCS cases with no comorbid FSD.ConclusionLower socioeconomic status was found to be associated with a higher risk of having MCS but not with MCS without FSD comorbidities. Due to the cross-sectional design of the study, we cannot determine whether social status is a determinant or a consequence of MCS.
Sexual dysfunction and associated factors in Thai patients with psychiatric disorders
Authors: Wainipitapong Sorawit , Chiddaycha Mayteewat , Charoenmakpol Natthaphon 
Published: March 2023

BackgroundSexual dysfunction is common among patients with psychiatric disorders but might be under-reported due to Asian sociocultural factors. Recognition of sexual dysfunction and associated factors in this vulnerable population would help clinicians properly assess and manage related conditions.AimsWe aimed to examine the prevalence of sexual dysfunction and its associated factors among patients with psychiatric disorders in Thailand.MethodsThis was a cross-sectional study. We enrolled participants aged 18 and older who visited the psychiatry clinic at King Chulalongkorn Memorial Hospital in Bangkok, Thailand between August 2020 and December 2021. Demographic and clinical data were assessed, and all psychiatric disorders and sexual dysfunctions were diagnosed by clinical interview using the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition. Statistical analysis was done to explore the association between sexual dysfunction and related factors.ResultsSexual dysfunction was diagnosed in 101 (50.0%) of the total 202 participants. The mean (standard deviation, SD) age was 30.2 (9.0) years, and the majority of patients were men (54.5%), single (81.2%), employed (47.5%) and had a coexisting depressive disorder (48.0%). Multivariable logistic regression analysis showed a significant association between sexual dysfunction and quality of life, unemployment, and the dosage of antidepressants and benzodiazepines.ConclusionsThe prevalence of sexual dysfunction among this population was relatively high. However, the findings may represent only a portion of affected psychiatric patients for others with sexual dysfunction symptoms but without functional impairment did not meet the diagnostic criteria for sexual dysfunction. Improvement of quality of life and optimising antidepressant/benzodiazepine dosage should be further investigated for promoting sexual function in patients with mental disorders.
Subannular repair in secondary mitral regurgitation with restricted leaflet motion during systole
Authors: Pausch Jonas , Harmel Eva , Reichenspurner Hermann , Kempfert Jörg , Kuntze Thomas , Owais Tamer , Holubec Tomas , Walther Thomas , Krane Markus , Vitanova Keti , Borger Michael Andrew , Eden Matthias , Hachaturyan Violetta , Bramlage Peter , Falk Volkmar , Girdauskas Evaldas 
Published: March 2023

ObjectiveVentricular secondary mitral regurgitation (SMR) (Carpentier type IIIb) results from left ventricular (LV) remodelling, displacement of papillary muscles and tethering of mitral leaflets. The most appropriate treatment approach remains controversial. We aimed to assess the safety and efficacy of standardised relocation of both papillary muscles (subannular repair) at 1-year follow-up (FU).MethodsREFORM-MR (Reform-Mitral Regurgitation) is a prospective, multicentre registry that enrolled consecutive patients with ventricular SMR (Carpentier type IIIb) undergoing standardised subannular mitral valve (MV) repair in combination with annuloplasty at five sites in Germany. Here, we report survival, freedom from recurrence of MR >2+, freedom from major adverse cardiac and cerebrovascular events (MACCEs), including cardiovascular death, myocardial infarction, stroke, MV reintervention and echocardiographic parameters of residual leaflet tethering at 1-year FU.ResultsA total of 94 patients (69.1% male) with a mean age of 65.1±9.7 years met the inclusion criteria. Advanced LV dysfunction (mean left ventricular ejection fraction 36.4±10.5%) and severe LV dilatation (mean left ventricular end-diastolic diameter 61.0±9.3 mm) resulted in severe mitral leaflet tethering (mean tenting height 10.6±3.0 mm) and an elevated mean EURO Score II of 4.8±4.6 prior to surgery. Subannular repair was successfully performed in all patients, without operative mortality or complications. One-year survival was 95.5%. At 12 months, a durable reduction of mitral leaflet tethering resulted in a low rate (4.2%) of recurrent MR >2+. In addition to a significant improvement in New York Heart Association (NYHA) class (22.4% patients in NYHA III/IV vs 64.5% patients at baseline, p<0.001), freedom from MACCE was observed in 91.1% of patients.ConclusionsOur study demonstrates the safety and feasibility of standardised subannular repair to treat ventricular SMR (Carpentier type IIIb) in a multicentre setting. By addressing mitral leaflet tethering, papillary muscle relocation results in very satisfactory 1-year outcomes and has the potential to durably restore MV geometry; nevertheless, long-term FU is mandatory.Trial registration numberNCT03470155.
Score to assess the probability of relapse in granulomatosis with polyangiitis and microscopic polyangiitis
Authors: Samson Maxime , Devilliers Hervé , Thietart Sara , Charles Pierre , Pagnoux Christian , Cohen Pascal , Karras Alexandre , Mouthon Luc , Terrier Benjamin , Puéchal Xavier , Guillevin Loic 
Published: March 2023

ObjectiveTo develop a score assessing the probability of relapse in granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA).MethodsLong-term follow-up data from GPA and MPA patients included in five consecutive randomised controlled trials were pooled. Patient characteristics at diagnosis were entered into a competing-risks model, with relapse as the event of interest and death the competing event. Univariate and multivariate analyses were computed to identify variables associated with relapse and build a score, which was then validated in an independent cohort of GPA or MPA patients.ResultsData collected from 427 patients (203 GPA, 224 MPA) at diagnosis were included. Mean±SD follow-up was 80.6±51.3 months; 207 (48.5%) patients experienced ≥1 relapse. Relapse risk was associated with proteinase 3 (PR3) positivity (HR=1.81 (95% CI 1.28 to 2.57); p<0.001), age ≤75 years (HR=1.89 (95% CI 1.15 to 3.13); p=0.012) and estimated glomerular filtration rate (eGFR) ≥30 mL/min/1.73 m² (HR=1.67 (95% CI 1.18 to 2.33); p=0.004) at diagnosis. A score, the French Vasculitis Study Group Relapse Score (FRS), from 0 to 3 points was modelised: 1 point each for PR3-antineutrophil cytoplasmic antibody positivity, eGFR ≥30 mL/min/1.73 m² and age ≤75 years. In the validation cohort of 209 patients, the 5-year relapse risk was 8% for a FRS of 0, 30% for 1, 48% for 2 and 76% for 3.ConclusionThe FRS can be used at diagnosis to assess the relapse risk in patients with GPA or MPA. Its value for tailoring the duration of maintenance therapy should be evaluated in future prospective trials.
Robust current-mode control of bridgeless single-switch SEPIC PFC converter
Authors: Al-Baidhani Humam , Sahib Abdullah 
Published: March 2023

In this paper, the nonlinear model of the bridgeless single-switch ac-dc single-ended primary-inductor converter (SEPIC) in discontinuous conduction mode is derived. In addition, a robust control method is introduced to accommodate the variations in input voltage and load current. The current-mode controlled power converter is designed to operate in buck and boost modes. The proposed closed-loop SEPIC converter is simulated in MATLAB to validate the design approach. The current-mode control scheme is also compared with the conventional voltage-mode controller. It is confirmed that the proposed control scheme exhibits precise tracking performance and enhanced transient response under large disturbances.
Investigating the specific absorption rate and antimicrobial activity of Mn0.25Fe2.75O4/Ag ferrogel based on carboxymethyl cellulose/polyvinyl alcohol composite polymer
Authors: Sunaryono Sunaryono , Chusna Nadiya Miftachul , Hidayat Muchlis Fajar , Choerullah Andy , Hidayat Samsul , Mufti Nandang , Aripriharta Aripriharta , Afandi Arif Nur , Rajagukguk Juniastel , Malek Nik Ahmad Nizam Nik , Taufiq Ahmad 
Published: March 2023

Carboxymethyl cellulose (CMC)/polyvinyl alcohol (PVA)-Mn0.25Fe2,75O4/Ag ferrogels was fabricated by using the freezing-thawing method. Mn0.25Fe2.75O4/Ag magnetic fillers of ferrogels were synthesized by using coprecipitation and chemical reduction methods. Characterization of Mn0.25Fe2.75O4/Ag nanopowders was carried out using X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) instrument, and antimicrobial activity. At the same time, CMC/PVA-Mn0.25Fe2.75O4/Ag ferrogels were characterized by using magnetothermal instrument. The XRD pattern of the magnetite filler shows the peaks by the Fe3O4 and Ag phases, respectively. The TEM image, there are Mn0.25Fe2.75O4 and Ag with particle size distributions of 5–7 and 10–13 nm, respectively. The hysterical curve from VSM characterization shows a decrease in the value of magnetization saturation from 16.9 to 14.7 emu/g along with the addition of Ag material. Antimicrobial activity was tested using the agar diffusion method. Mn0.25Fe2.75O4/Ag samples showed very good results indicated by the widening of the zone of inhibition around the sample. Thus, CMC/PVA-Mn0.25Fe2.75O4/Ag ferrogels has the potential application to be applied in hyperthermia therapy as well as antimicrobial materials.
Mitochondrial pyruvate dehydrogenase kinases contribute to platelet function and thrombosis in mice by regulating aerobic glycolysis
Authors: Flora Gagan D. , Nayak Manasa K. , Ghatge Madankumar , Kumskova Mariia , Patel Rakesh B. , Chauhan Anil K. 
Published: March 2023

Abstract
Resting platelets rely on oxidative phosphorylation (OXPHOS) and aerobic glycolysis (conversion of glucose to lactate in the presence of oxygen) for their energy requirements. In contrast, platelet activation exhibits an increased rate of aerobic glycolysis relative to OXPHOS. Mitochondrial enzymes pyruvate dehydrogenase kinases (PDKs) phosphorylate the pyruvate dehydrogenase (PDH) complex to inhibit its activity, thereby diverting the pyruvate flux from OXPHOS to aerobic glycolysis upon platelet activation. Of 4 PDK isoforms, PDK2 and PDK4 (PDK2/4) are predominantly associated with metabolic diseases. Herein, we report that the combined deletion of PDK2/4 inhibits agonist-induced platelet functions, including aggregation, integrin αIIbβ3 activation, degranulation, spreading, and clot retraction. In addition, collagen-mediated PLCγ2 phosphorylation and calcium mobilization were significantly reduced in PDK2/4−/− platelets, suggesting impaired GPVI signaling. The PDK2/4−/− mice were less susceptible to FeCl3-induced carotid and laser-induced mesenteric artery thrombosis without any effect on hemostasis. In adoptive transfer experiments, thrombocytopenic hIL-4Rα/GPIbα-transgenic mice transfused with PDK2/4−/− platelets exhibited less susceptibility to FeCl3 injury–induced carotid thrombosis compared with hIL-4Rα/GPIbα-Tg mice transfused with WT platelets, suggesting a platelet-specific role of PDK2/4 in thrombosis. Mechanistically, the inhibitory effects of PDK2/4 deletion on platelet function were associated with reduced PDH phosphorylation and glycoPER in activated platelets, suggesting that PDK2/4 regulates aerobic glycolysis. Finally, using PDK2 or PDK4 single KO mice, we identified that PDK4 plays a more prominent role in regulating platelet secretion and thrombosis compared with PDK2. This study identifies the fundamental role of PDK2/4 in regulating platelet functions and identifies the PDK/PDH axis as a potentially novel antithrombotic target.
Imaging and histological evaluation of the long head of the biceps tendon in the presence of different types of rotator cuff tears
Authors: Kajiwara Daisuke , Ochiai Nobuyasu , Hashimoto Eiko , Hirosawa Naoya , Akimoto Koji , Nojima Daisuke , Shimada Yohei , Ise Shohei , Ohtori Seiji 
Published: March 2023

Abstract
Background
A comparison of changes in the long head of the biceps tendon for different types of rotator cuff tears has not been previously performed. Furthermore, the correlation between the thickening and degeneration of the long head of the biceps tendon and the cause of these changes have not been fully clarified. We evaluated the relationship between degenerative changes in the long head of the biceps tendon and rotator cuff tears in a rat model using imaging and histology.

Methods
Ninety-six 12-week-old Sprague–Dawley rats were divided into anterior (subscapularis tear), anterosuperior (subscapularis, supraspinatus, and infraspinatus tears), superior (supraspinatus and infraspinatus tears), and control groups. The long head of the biceps tendon was harvested at 4 or 12 weeks postoperatively. The cross-sectional areas of the intra- and extra-capsular components of the tendon were measured using micro-computed tomography, and the affected/normal ratio of the cross-sectional area was calculated. Masson’s trichrome staining and Alcian blue staining were performed for histologic analysis, with degenerative changes described using the modified Bonar scale. The correlation between the affected/normal ratio and Bonar scores was evaluated.

Results
The affected/normal ratio was higher for the anterior and anterosuperior groups than for the control group at 4 and 12 weeks. The ratio increased for the intra-articular portion in the superior group and for both the intra- and extra-articular portions in the anterior and anterosuperior groups. Degeneration considerably progressed in the anterior and anterosuperior groups compared with the control group from weeks 4 to 12 and was greater in the intra- than in the extra-articular portion. The ratio correlated with extracellular matrix score.

Conclusions
Subscapularis tears were associated with progressive thickening and degeneration of the long head of the biceps tendon at 4 and 12 weeks postoperatively, which was more significant in the intra- than in the extra-articular portion. Histologic evaluation indicated that the extracellular matrix likely caused these degenerative changes.