bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping of centriolar proteins onto the post-embryonic lineage ofC. elegans
Authors: Kalbfuss Nils , Berger Antonin , Gönczy Pierre 
Published: May 2023

AbstractCentrioles, together with the surrounding peri-centriolar material (PCM), constitute the centrosome, a major microtubule-organizing center of animal cells. Despite being critical in many cells for signaling, motility and division, centrioles can be eliminated in some systems, including in the vast majority of differentiating cells during embryogenesis inCaenorhabditis elegans. Whether the cells retaining centrioles in the resulting L1 larvae do so because they lack an activity that eliminates centrioles in the other cells is not known. Moreover, the extent to which centrioles and PCM remain present in later stages of worm development, when all cells but those of the germ line are terminally differentiated, is not known. Here, by fusing cells that lack centrioles with cells that retain them, we established that L1 larvae do not possess a diffusible elimination activity sufficient to remove centrioles. Moreover, analyzing PCM core proteins in L1 larval cells that retain centrioles, we found that some such proteins, but not all, are present as well. Furthermore, we uncovered that foci of centriolar proteins remain present in specific terminally differentiated cells of adult hermaphrodites and males, in particular in the somatic gonad. Correlating the time at which cells were born with the fate of their centrioles revealed that it is not cell age, but instead cell fate, that determines whether and when centrioles are eliminated. Overall, our work maps the localization of centriolar and PCM core proteins in the post-embryonicC. eleganslineage, thereby providing an essential blueprint for uncovering mechanisms modulating their presence and function.
Phototriggered profiling of receptor-proximal proteins in vivo in minutes
Authors: Takato Mikiko , Sakamoto Seiji , Nonaka Hiroshi , Tamura Tomonori , Hamachi Itaru 
Published: May 2023

AbstractNeurotransmitter receptors are regulated by an extensive and dynamic network of protein-protein interactions. Understanding how these networks control synaptic transmission and give rise to higher-order brain functions necessitates their investigation in the live mammalian brain. However, tools available for this purpose lack the temporal resolution necessary to capture rapid changes in the interactome in live animals and require potentially disruptive genetic modifications to the animal under study. Here, we describe a method for light-activated proximity labelling in the live mouse brain that relies solely on small-molecule reagents and achieves a minute-order temporal resolution. Named PhoxID (photooxidation-driven proximity labelling for protein identification), this method involves selectively tethering a chemical photosensitiser to neurotransmitter receptors of interest and enabled us to identify characteristic as well as less studied interactors of the endogenous α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) and the ψ-aminobutyric acid type A receptor (GABAAR) with just minutes of in-brain green light irradiation. Furthermore, PhoxID’s temporal precision allowed us to capture molecular snapshots of the AMPAR-proximal proteome in the postnatal developing cerebellum, leading to the discovery of age-dependent shifts. Overall, this work establishes a highly flexible and generalisable platform to study receptor interactomes and proximal microenvironments in genetically intact specimens with an unprecedented temporal resolution.
RNA Targets and Physiological Role of Topoisomerase 3β
Authors: Teimuri Shohreh , Suter Beat 
Published: May 2023

AbstractTopoisomerase 3β (Top3β) works not only on DNA but also RNA. We isolated and identified the naturally cross-linked RNA targets of Drosophila Top3β from an early embryonic stage that contains almost exclusively maternal mRNAs. Favorite targets were longer RNAs, RNAs with many splice sites, and RNAs that become localized in large cells. Demonstrating the importance of its enzymatic activity, Top3β without the hydroxyl group that makes the covalent bond to the RNA, did not allow normal expression and subcellular localization of tested gene products of the identified targets.Top3βis not essential for development to the adult stage but to maintain the morphology of the neuromuscular junction in adult flies and to prevent premature loss of coordinated movement and aging. Alterations in humanTop3βhave been associated with several neurological diseases and cancers. The homologs of genes and (pre)mRNAs mis-expressed in these conditions show the same characteristics identified in the DrosophilaTop3βtargets, suggesting that Drosophila could model the function of humanTop3β. Indeed, anin vivotest of this model showed that the enzymatic activity of Top3β reduces the neurodegeneration caused by the human (G4C2)49RNA. Together, these studies show thatTop3βplays important roles in normal gene expression, particularly for large genes and long and complex transcripts that need to be transported and translationally controlled. Its absence stresses the cells, seemingly increasing the chances of contracting various neuronal and neurodegenerative diseases and cancers.
Changes in the Type 2 diabetes gut mycobiome associate with metformin treatment across populations
Authors: Syoc Emily Van , Nixon Michelle Pistner , Silverman Justin D. , Luo Yuhong , Gonzalez Frank J. , Elbere Ilze , Klovins Janis , Patterson Andrew D. , Rogers Connie J. , Ganda Erika 
Published: May 2023

ABSTRACTThe human gut teems with a diverse ecosystem of microbes, yet non-bacterial portions of that community are overlooked in studies of metabolic diseases firmly linked to gut bacteria. Type 2 diabetes mellitus (T2D) associates with compositional shifts in the gut bacterial microbiome and fungal mycobiome, but whether T2D and/or pharmaceutical treatments underpin the community change is unresolved. To differentiate these effects, we curated a gut mycobiome cohort to-date spanning 1,000 human samples across 5 countries and a murine experimental model. We use Bayesian multinomial logistic normal models to show that metformin and T2D both associate with shifts in the relative abundance of distinct gut fungi. T2D associates with shifts in the Saccharomycetes and Sordariomycetes fungal classes, while the generaFusariumandTetrapisiporamost consistently associate with metformin treatment. We confirmed the impact of metformin on individual gut fungi by administering metformin to healthy mice. Thus, metformin and T2D account for subtle, but significant and distinct variation in the gut mycobiome across human populations. This work highlights for the first time that oral pharmaceuticals can confound associations of gut fungi with T2D and warrants the need to consider pharmaceutical interventions in investigations of linkages between metabolic diseases and gut microbial inhabitants.
Prenatal experience with language shapes the brain
Authors: Mariani Benedetta , Nicoletti Giorgio , Barzon Giacomo , Ortíz Barajas María Clemencia , Shukla Mohinish , Guevara Ramón , Suweis Samir Simon , Gervain Judit 
Published: May 2023

AbstractHuman infants acquire language with striking ease compared to adults, but the neural basis of their remarkable brain plasticity for language remains little understood. Applying a scaling analysis of neural oscillations for the first time to address this question, we show that newborns’ electrophysiological activity exhibits increased long-range temporal correlations after stimulation with speech, particularly in the prenatally heard language, indicating the early emergence of brain specialization for the native language.
What are the key factors of enterprises' greenwashing behaviors under multi-agent interaction? A grey-DEMATEL analysis from Chinese construction materials enterprises
Authors: Li Xingwei , Li Jingru , He Jinrong , Huang Yicheng , Liu Xiang , Dai Jiachi , Shen Qiong 
Published: May 2023

PurposeThe surging market demand for green construction materials has brought opportunities for construction materials enterprises' greenwashing behavior (GWB). This study aims to establish the causal relationship among the influencing factors of GWB and reveal the key influencing factors from the perspective of Chinese construction materials enterprises under multi-agent interactions.Design/methodology/approachThis study is based on stakeholder theory, resource-based theory and the green development behavior and performance of industrial enterprises (GDBP-IE). First, with the literature analysis, an index framework of the influencing factors of enterprises' GWB was constructed from five dimensions (including 15 factors): environmental regulation, public scrutiny, market environment, corporate resources and corporate green development (GD) performance. Second, the interactive relationship among influencing factors was obtained by a questionnaire survey. Finally, the data are processed and analyzed with the grey-DEMATEL (Decision-making Trial and Evaluation Laboratory) method.FindingsAmong the factors, corporate information transparency has the greatest impact on the other factors, and consumer green preferences are most influenced by others. The most critical and important factor is the corporate social performance factor. In China, corporate social performance, corporate information transparency, corporate size and media supervision are the key factors influencing the GWB of construction materials enterprises.Originality/valueThis study provides a new perspective on the literature related to GWB by considering multi-agent interactions and extends the evidence from the construction materials industry for research on the drivers or influencing factors of enterprises' bad environmental behavior. Furthermore, it adds insights from China for further research on the governance strategies of GWB in other countries.
Perception of sponge city for achieving circularity goal and hedge against climate change: a study on Weibo
Authors: Zeng Liyun , Li Rita Yi Man , Zeng Huiling , Song Lingxi 
Published: May 2023

Purpose
Global climate change speeds up ice melting and increases flooding incidents. China launched a sponge city policy as a holistic nature-based solution combined with urban planning and development to address flooding due to climate change. Using Weibo analytics, this paper aims to study public perceptions of sponge city.

Design/methodology/approach
This study collected 53,586 sponge city contents from Sina Weibo via Python. Various artificial intelligence tools, such as CX Data Science of Simply Sentiment, KH Coder and Tableau, were applied in the study.

Findings
76.8% of public opinion on sponge city were positive, confirming its positive contribution to flooding management and city branding. 17 out of 31 pilot sponge cities recorded the largest number of sponge cities related posts. Other cities with more Weibo posts suffered from rainwater and flooding hazards, such as Xi'an and Zhengzhou.

Originality/value
To the best of the authors’ knowledge, this study is the first to explore the public perception of sponge city in Sina Weibo.
Linking employees’ challenge-hindrance appraisals toward AI to service performance: the influences of job crafting, job insecurity and AI knowledge
Authors: He Changqing , Teng Rongrong , Song Jun 
Published: May 2023

Purpose
This study aims to explore the associations linking employees’ challenge-hindrance appraisals toward artificial intelligence (AI) to service performance while considering the dual mediating roles of job crafting and job insecurity, as well as the moderating role of AI knowledge.

Design/methodology/approach
A survey was administered to a sample of 297 service industry employees. This study examined all the hypotheses with Mplus 8.0.

Findings
This study confirms that challenge appraisal toward AI has an indirect positive influence on service performance via job crafting (motivation process), whereas hindrance appraisal toward AI has an indirect negative influence on service performance via job insecurity (strain process). Meanwhile, AI knowledge, serving as a key personal resource, could strengthen the positive impacts of challenge appraisal toward AI on job crafting and of hindrance appraisal toward AI on job insecurity.

Practical implications
Organizational decision-makers should first survey employees’ appraisals toward AI and then adopt targeted managerial strategies. From the perspective of service industry employees, employees should adopt proactive coping strategies and enrich their knowledge of AI to meet the challenges brought by this technology.

Originality/value
The primary contribution of this study is that we enrich the literature on AI by exploring the dual mediators (i.e. job crafting and job insecurity) through which AI awareness affects service performance. Moreover, this study advances our understanding of when appraisals toward AI influence job outcomes by identifying the moderating role of AI knowledge.
Local government's failure to recover loans from groups of women, youth, and people with disabilities in Tanzania
Authors: Rugeiyamu Rogers 
Published: May 2023

PurposeThe purpose of this study is to assess reasons behind experienced challenges by local government authorities (LGAs) in operating Women, Youth and People with Disabilities Fund (WYDF) in Tanzania. Specifically, it assesses the reasons behind failures to recover loan by LGAs and groups of Women, Youth and People with Disability (WYPWD).Design/methodology/approachThe qualitative approach was recruited in this study involving Tunduru District Council as a case study. Data were collected through Interviews, Focus Group Discussion and Documentary Review. Interviews were administered to Community Development Officers (CDOs) while FGD to WYPWD groups. Reviewed documents include laws, regulations and publications on social development funds. Data were analyzed using content analysis approach and backed up by quotations during presentation.FindingsFailures to recover loans from beneficiaries is attributed to weaknesses of both groups and LGAs. LGAs suffer from lack of capability to manage the fund, poor governance practices and misuse of public funds, and groups lack awareness of the fund's goals.Research limitations/implicationsDue to experienced challenges, efforts by groups and LGAs to reclaim loan have been unsuccessful, which has prevented the fund from achieving its goals.Practical implicationsThe central government should concentrate on ongoing LGAs capacity building so that they can successfully handle the fund, it is advised for improvement. Again, LGAs should establish an information system linked with groups to track their projects implementation. Once more, groups should be informed about the purpose of creating the fund and the advantages of the loan to them and to local economic development (LED). Furthermore, groups need entrepreneurial abilities to be able to participate in businesses that they can manage. Moreover, organizations should receive ongoing education so that they may repay the loan voluntarily.Social implicationsCommunity awareness on the aims of the fund should be provided to impact LED.Originality/valueRecommendations given can be applied by other developing countries struggling to uplift citizens economically through social development funds.
Measuring human IT agility and firms' digitalization using POSET: evidence from Italy
Authors: Rubino Michele , Maggino Filomena , Antonicelli Margaret 
Published: May 2023

PurposeThe aim of this study is to provide a detailed picture of the digitalization propensity and human IT agility of Italian SMEs, verifying whether companies are pursuing coherent and reliable choices for these dimensions and whether digitalization choices affect human IT agility.Design/methodology/approachUsing a POSET approach, this study constructs two nonaggregative multidimensional indicators of human information technology (IT) agility and firms' digitalization. The analysis is based on the microdata provided by ISTAT relating to 4,682 Italian manufacturing companies.FindingsThe results show the existence of a strong relationship between digitalization propensity and human IT agility. However, the analysis shows that companies are characterized by a low level of digitalization propensity and human IT agility. At the same time, the findings highlighted that the managerial choices adopted by companies appear to be inconsistent with respect to the two multidimensional indicators.Practical implicationsThis study has important implications for managers and policymakers by suggesting acting specific policies to promote a better implementation of digitalization that considers the key role of human IT agility.Originality/valueThis study contributes to the existing literature on organizational agility and digitalization by providing a detailed picture of the Italian manufactured SMEs. At the same time, the POSET approach allows to aggregate a lot of information in one or more indicators without neglecting the value of each dimension faced with the extreme heterogeneity of companies' profiles.
Effect of polymer additives on the tribological performance of soybean oil
Authors: Guru Soumya Ranjan , Venugopal Chetla , Sarangi Mihir 
Published: May 2023

Purpose
This study aims to investigate the behavior of vegetable oil with two additives. Base oil’s tribological qualities can be improved with the help of several additions. In the present investigation, soybean oil is served as the foundational oil due to its eco-friendliness and status as a vegetable oil with two additives, named polytetrafluoroethylene (PTFE) and molybdenum disulfide (MoS2).

Design/methodology/approach
As additives, PTFE and MoS2 are used; PTFE is renowned for its anti-friction (AF) properties, while MoS2 is a solid lubricant with anti-wear (AW) properties. This investigation examines the synergistic impact of AF and AW additions in vegetable oil. The lubricity of the base oil is measured by using a four-ball tester, and the wear properties of the oil at different additive amounts are determined by using a universal tribometer.

Findings
PTFE (at 5 Wt.%) and MoS2 (at 1 Wt.%) were found to improve the tribological performance of the base oil. The weld load is significantly increased when 5 Wt.% of PTFE + MoS2 is added to the base oil.

Originality/value
A better tribological characteristic can be achieved by combining additives that amount to less than 1% of the base oil. In experiments with highly concentrated MoS2, the adequate pressure improved dramatically, but the lubricant’s tribological characteristics did not.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-11-2022-0321/
Ceasing to communicate public family firm identity: the decoupling of internally experienced and externally communicated identities
Authors: Boers Börje , Ljungkvist Torbjörn , Brunninge Olof 
Published: May 2023

PurposeThe purpose of this study is to explore how the family firm identity is affected when it is no longer publicly communicated.Design/methodology/approachA case study approach was used to follow a third-generation family business, a large Swedish home electronics firm that acquired a competitor and, initially, continued using its family firm identity after the acquisition. This study longitudinally tracks the company and its owning family using archival data combined with interviews.FindingsThe case company decided to stop communicating their identity as a family business. Such a move initially appears counterintuitive, since it potentially threatens the family firm identity and leads the firm to forgo other advantages, e.g. in branding. However, the decision was based on arguments that were rational from a business perspective, leading to a decoupling of family and firm identity.Originality/valueThis study contributes to the literature by showing a decoupling of internally experienced and externally communicated identities. It further contributes to the understanding of the family firm identity concept.
IBM's Ginni Rometty: portrait of a leader learning to use “Good Power”
Authors: Leavy Brian 
Published: May 2023

Purpose
This masterclass examines former IBM CEO Ginni Rometty's personal memoir of her life and career as a study of authentic leadership in action, and her concept of “good power” through which she distills the major insights on leadership she gleaned along the way.

Design/methodology/approach
This masterclass examines the insights from a former IBM CEO's “memoir with purpose” as an example of the classic perspective on authentic leadership developed in the “True North” series of studies by Harvard's Bill George and his research associates over two decades.

Findings
Ginni Rometty's “good power” concept and principles, as developed in her “memoir with purpose,” represent a practical guide for aspiring leaders seeking to become a positive force for change, and they make a valuable addition to the literature on authentic leadership.

Research limitations/implications
Her “good power” concept and experience-based principles, as developed in her “memoir with purpose” make a valuable addition to the literature on authentic leadership.

Practical implications
Her “good power” concept and principles, as developed in her “memoir with purpose,” represent a practical guide for aspiring leaders seeking to become a positive force for change.

Social implications
Her memoir, with its conception of “good power,” strongly encourages corporate leaders to adopt a “stakeholder perspective” and become more active and outspoken, forces for positive change in the wider societies within which they operate.

Originality/value
Reflective memoirs as analytical as this one, from someone who has led at the highest level in the business world, are still rare in the genre.
Six Sigma application in an Irish meat processing plant to improve process yields
Authors: Gilligan Rebecca , Moran Rachel , McDermott Olivia 
Published: May 2023

PurposeThis study aims to utilise Six Sigma in an Irish-based red meat processor to reduce process variability and improve yields.Design/methodology/approachThis is a case study within an Irish meat processor where the structured Define, Measure, Analyse, Improve and Control (DMAIC) methodology was utilised along with statistical analysis to highlight areas of the meat boning process to improve.FindingsThe project led to using Six Sigma to identify and measure areas of process variation. This resulted in eliminating over-trimming of meat cuts, improving process capabilities, increasing revenue and reducing meat wastage. In addition, key performance indicators and control charts, meat-cutting templates and smart cutting lasers were implemented.Research limitations/implicationsThe study is one of Irish meat processors' first Six Sigma applications. The wider food and meat processing industries can leverage the learnings to understand, measure and minimise variation to enhance revenue.Practical implicationsOrganisations can use this study to understand the benefits of adopting Six Sigma, particularly in the food industry and how measuring process variation can affect quality.Originality/valueThis is the first practical case study on Six sigma deployment in an Irish meat processor, and the study can be used to benchmark how Six Sigma tools can aid in understanding variation, thus benefiting key performance metrics.