bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential oil composition and antimicrobial potential of aromatic plants grown in the mid-hill conditions of the Western Himalayas
Authors: Rathore Shalika , Mukhia Srijana , Kumar Rakshak , Kumar Rakesh 
Published: March 2023

AbstractEssential oils are highly concentrated natural extracts obtained from plants, rich in bioactive constituents with antimicrobial properties, but the distinctive climate of the Western Himalayan region influences the same. Aromatic and medicinal plants, viz., Origanum majorana, Origanum vulgare, Cymbopogon winterianus, Pelargonium graveolens, and Nepeta cataria were grown in the foothills of the Western Himalayan condition and evaluated for essential oil content, composition, and their effect on some of the most common pathogenic microorganisms. The essential oil content (%) was 0.77, 0.45, 1.37, 0.15 and 0.17% in O. majorana, O. vulgare, C. winterianus, P. graveolens, and N. cataria, respectively. The major essential oil constituents of the isolated oils were terpinen-4-ol, thymol, citronellal, citronellol, and nepetalactone, contributing 41.24%, 31.81%, 43.13%, 43.35% and 91.43% in O. majorana, O. vulgare, C. winterianus, P. graveolens, and N. cataria, respectively. Well-diffusion assay revealed that the essential oil of O. majorana and O. vulgare was active against both the tested Gram-positive, viz., Bacillus subtilis MTCC 121, Micrococcus luteus MTCC 2470, and Staphylococcus aureus MTCC 96; and Gram-negative, viz., Escherichia coli MTCC 43, Klebsiella pneumoniae MTCC 109, and Pseudomonas aeruginosa MTCC 2453 bacteria, while the essential oil of C. winterianus, P. graveolens, and N. cataria showed activity against only some Gram-positive bacteria. Minimum inhibitory concentration (v/v) values indicated the highest efficacy of O. majorana essential oil against B. subtilis (0.5%), M. luteus (1%), and S. aureus (1%), while O. vulgare was most efficient to E. coli (2%) and K. pneumoniae (2%). C. winterianus essential oil did not inhibit any bacterial strains. M. luteus was susceptible to the essential oil of P. graveolens (1%) and N. cataria (0.5%) at low concentrations. Present findings showed the association between the chemical constituents’ profile of isolated essential oils from the Himalayan region and their antimicrobial activity, indicating their perspective to be utilized as antibacterial means.
Contour device implantation versus coil embolization for treatment of narrow neck intracranial aneurysms
Authors: Mostafa Karim , Bueno Neves Fernando , Gärtner Friederike , Peters Sönke , Hensler Johannes , Larsen Naomi , Klintz Tristan , Mahnke Justus , Jansen Olav , Wodarg Fritz 
Published: March 2023

AbstractThe novel Contour device is an intrasaccular flow disruption device designed for treatment of intracranial wide-neck bifurcation aneurysms. Outside its original purpose, Contour implantation can be used to treat aneurysms with a higher dome-to-neck ratio which would be suitable for conventional unassisted coil embolization. We compared both techniques in a retrospective single-center analysis. A total of 42 aneurysms from 42 patients with a dome-to-neck ratio of 1.6 or higher were included in this study. Data on technical success, implantation times, radiation dosages, procedural complications, reinterventions and recurrences were gathered and compared. Technical success was achieved in all cases with both techniques. Aneurysm embolization was achieved significantly faster in the Contour group compared to coiling (Overall p = 0.0002; r = 0.580; acute setting: p = 0.005, r = 0.531; elective setting: p = 0.002, r = 0.607). Significantly less radiation dosage was applied in the Contour group (Overall p = 0.002; r = 0.478; acute group p = 0.006; r = 0.552; elective group p = 0.045; r = 0.397). The number of complications was higher in the coiling group compared to the Contour group (Coiling 7/21 (33,3%); Contour 3/21 (14.3%). There was a higher rate of reinterventions in the coiling group (7.6% vs 21.4%). Outside its original intention, the Contour device seems to be a safe and fast alternative to coil embolization for the treatment of narrow-neck-aneurysms.
A laminar forced convection via transport of water–copper–aluminum hybrid nanofluid through heated deep and shallow cavity with Corcione model
Authors: Memon Abid. A. , Memon M. Asif , Fenta Amsalu 
Published: March 2023

AbstractThe article explores how fluid flows and heat transfers in both deep and shallow cavities when using a nanofluid made of water, copper, and aluminum oxide. The study applies the Corcione model to hybrid nanofluids, which considers viscosity, conductivity, and the size of the nanoparticle, temperature, and Reynolds number. The cavity is connected to a rectangular channel, with the cavity's length being half the total length of the enclosure, and the aspect ratio (cavity height divided by height of the channel) is tested from 1 to 3. The study uses the Navier–Stokes equation and energy equation in two dimensions, along with finite element-based software, COMSOL 5.6, to simulate the combination of fluid flow and heat transmission. The results show a circular distribution of temperature in the cavity, and the average temperature drops as the volume fraction of copper upsurges. However, both the Reynolds number and volume fraction of copper improve the average Nusselt number, which shows how well the fluid transfers heat, along the cavity's middle line. The percentage change in the average Nusselt number decreases as the aspect ratio increases, indicating improved conduction.
Evidence of a cognitive bias in the quantification of COVID-19 with CT: an artificial intelligence randomised clinical trial
Authors: Bercean Bogdan A. , Birhala Andreea , Ardelean Paula G. , Barbulescu Ioana , Benta Marius M. , Rasadean Cristina D. , Costachescu Dan , Avramescu Cristian , Tenescu Andrei , Iarca Stefan , Buburuzan Alexandru S. , Marcu Marius , Birsasteanu Florin 
Published: March 2023

AbstractChest computed tomography (CT) has played a valuable, distinct role in the screening, diagnosis, and follow-up of COVID-19 patients. The quantification of COVID-19 pneumonia on CT has proven to be an important predictor of the treatment course and outcome of the patient although it remains heavily reliant on the radiologist's subjective perceptions. Here, we show that with the adoption of CT for COVID-19 management, a new type of psychophysical bias has emerged in radiology. A preliminary survey of 40 radiologists and a retrospective analysis of CT data from 109 patients from two hospitals revealed that radiologists overestimated the percentage of lung involvement by 10.23 ± 4.65% and 15.8 ± 6.6%, respectively. In the subsequent randomised controlled trial, artificial intelligence (AI) decision support reduced the absolute overestimation error (P < 0.001) from 9.5% ± 6.6 (No-AI analysis arm, n = 38) to 1.0% ± 5.2 (AI analysis arm, n = 38). These results indicate a human perception bias in radiology that has clinically meaningful effects on the quantitative analysis of COVID-19 on CT. The objectivity of AI was shown to be a valuable complement in mitigating the radiologist’s subjectivity, reducing the overestimation tenfold.Trial registration: https://Clinicaltrial.gov. Identifier: NCT05282056, Date of registration: 01/02/2022.
Different molecular pathways are disrupted in Pyoderma gangrenosum patients and are associated with the severity of the disease
Authors: Moura Ronald Rodrigues , Brandão Lucas , Moltrasio Chiara , Agrelli Almerinda , Tricarico Paola Maura , Maronese Carlo Alberto , Crovella Sergio , Marzano Angelo Valerio 
Published: March 2023

AbstractPyoderma gangrenosum (PG) is a rare inflammatory skin disease classified within the spectrum of neutrophilic dermatoses. The pathophysiology of PG is yet incompletely understood but a prominent role of genetics facilitating immune dysregulation has been proposed. This study investigated the potential contribution of disrupted molecular pathways in determining the susceptibility and clinical severity of PG. Variant Enrichment Analysis, a bioinformatic pipeline applicable for Whole Exome Sequencing data was performed in unrelated PG patients. Eleven patients were enrolled, including 5 with unilesional and 6 with multilesional PG. Fourteen pathways were exclusively enriched in the “multilesional” group, mainly related to immune system (i.e., type I interferon signaling pathway), cell metabolism and structural functions. In the “unilesional” group, nine pathways were found to be exclusively enriched, mostly related to cell signaling and cell metabolism. Genetically altered pathways involved in immune system biology and wound repair appear to be nodal pathogenic drivers in PG pathogenesis.
Recruit-aged adults may preferentially weight task goals over deleterious cost functions during short duration loaded and imposed gait tasks
Authors: Krajewski Kellen T. , Johnson Camille C. , Ahamed Nizam U. , Moir Gavin L. , Mi Qi , Flanagan Shawn D. , Anderst William J. , Connaboy Chris 
Published: March 2023

AbstractOptimal motor control that is stable and adaptable to perturbation is reflected in the temporal arrangement and regulation of gait variability. Load carriage and forced-marching are common military relevant perturbations to gait that have been implicated in the high incidence of musculoskeletal injuries in military populations. We investigated the interactive effects of load magnitude and locomotion pattern on motor variability, stride regulation and spatiotemporal complexity during gait in recruit-aged adults. We further investigated the influences of sex and task duration. Healthy adults executed trials of running and forced-marching with and without loads at 10% above their gait transition velocity. Spatiotemporal parameters were analyzed using a goal equivalent manifold approach. With load and forced-marching, individuals used a greater array of motor solutions to execute the task goal (maintain velocity). Stride-to-stride regulation became stricter as the task progressed. Participants exhibited optimal spatiotemporal complexity with significant but not meaningful differences between sexes. With the introduction of load carriage and forced-marching, individuals relied on a strategy that maximizes and regulates motor solutions that achieve the task goal of velocity specifically but compete with other task functions. The appended cost penalties may have deleterious effects during prolonged execution, potentially increasing the risk of musculoskeletal injuries.
Prenatal nicotine exposure alters gene expression profiles of neurons in the sub-regions of the VTA during early postnatal development
Authors: McGill Lindsey D. , Avci Naze G. , Kazemi Tina , Sunaga Yoshinori , Akay Yasemin M. , Akay Metin 
Published: March 2023

AbstractBrain growth occurs during the first 2 weeks of postnatal development in rats. This developmental period is equivalent to the third trimester of human gestation. Dendritic arborization, axonal growth, and gliogenesis are observed along with a strong maturation of neurotransmission during this critical development period. Furthermore, nicotine exposure during early development causes deficiencies in sensory and cognitive processing in adults. In this study, we further investigated the gene expression of neuron groups and the influence of perinatal nicotine exposure on gene expressions of neurons within the sub-regions of the ventral tegmental area (VTA) in 1 week, 2 week and 3-week-old rat pups. We exposed pregnant rats to nicotine perinatally on gestational day 7 through postnatal day 14. Pups are exposed to nicotine during pregnancy and through breastfeeding to investigate its effect in rat pups during early neuronal development. Real time PCR was used to find the relative expressions of gamma-aminobutyric acid (GABA), dopamine, and glutamate neuron markers within the three sub-regions of the VTA including the parabrachial pigmented nucleus (PBP), parainterfascicular (PIF), and paranigral nucleus (PN). Our results indicated that during early maturation, the dopamine marker tyrosine hydroxylase (TH) showed a consistently increased significance in PN sub-region compared to PIF and PBP. These results suggest that following perinatal nicotine exposure, VTA dopamine neurons, especially within the PN sub-region, are significantly excited starting from birth.
Increasing the melting temperature of VHH with the in silico free energy score
Authors: Tomimoto Yusuke , Yamazaki Rika , Shirai Hiroki 
Published: March 2023

AbstractVHH, the antigen-binding fragment of a heavy chain-only antibody, is a useful component of antibody-based therapeutics. Thermal stability, represented by the melting temperature (Tm), is one of the key factors affecting the developability of antibody-based therapeutics. In this study, we examined whether the in silico free energy score dStability can be used to design mutants with improved Tm compared to the anti-lysozyme VHH, D3-L11. After verifying that exhaustive mutagenesis was inefficient for improving Tm, we performed a two-round rational approach that combined dStability calculations with a small number of experiments. This method improved the Tm by more than 5 °C in several single mutants including A79I. It reduced the affinity for the antigen by less than 1.6-fold. We speculate that stabilization of A79I required exquisite compatibility among neighboring residues to fill in the internal cavity in the protein. Given that we identified only one mutation that could simultaneously improve Tm and almost maintain affinity, we concluded that achieving both is extremely difficult, even with single mutations that are not located in the paratope. Therefore, we recommend using a variety of approaches when trying to achieve such a feat. Our method will be a useful complementary approach to other existing methods.
The 8-bromobaicalein inhibited the replication of dengue, and Zika viruses and targeted the dengue polymerase
Authors: Boonyasuppayakorn Siwaporn , Saelee Thanaphon , Huynh Thao Nguyen Thanh , Hairani Rita , Hengphasatporn Kowit , Loeanurit Naphat , Cao Van , Vibulakhaophan Vipanee , Siripitakpong Panattida , Kaur Parveen , Chu Justin Jang Hann , Tunghirun Chairat , Choksupmanee Opas , Chimnaronk Sarin , Shigeta Yasuteru , Rungrotmongkol Thanyada , Chavasiri Warinthorn 
Published: March 2023

AbstractDengue and Zika viruses are mosquito-borne flaviviruses burdening millions every year with hemorrhagic fever and neurological symptoms. Baicalein was previously reported as a potential anti-flaviviral candidate and halogenation of flavones and flavanones potentiated their antiviral efficacies. Here, we reported that a chemically modified 8-bromobaicalein effectively inhibited all dengue serotypes and Zika viruses at 0.66–0.88 micromolar in cell-based system. The compound bound to dengue serotype 2 conserved pocket and inhibited the dengue RdRp activity with 6.93 fold more than the original baicalein. Moreover, the compound was mildly toxic against infant and adult C57BL/6 mice despite administering continuously for 7 days. Therefore, the 8-bromobaicalein should be investigated further in pharmacokinetics and efficacy in an animal model.
Phosphate induces inflammation and exacerbates injury from cigarette smoke in the bronchial epithelium
Authors: Bollenbecker Seth , Heitman Kylie , Czaya Brian , Easter Molly , Hirsch Meghan June , Vang Shia , Harris Elex , Helton E. Scott , Barnes Jarrod W. , Faul Christian , Krick Stefanie 
Published: March 2023

AbstractAn elevation in serum phosphate—also called hyperphosphatemia—is associated with reduced kidney function in chronic kidney disease (CKD). Reports show CKD patients are more likely to develop lung disease and have poorer kidney function that positively correlates with pulmonary obstruction. However, the underlying mechanisms are not well understood. Here, we report that two murine models of CKD, which both exhibit increased serum levels of phosphate and fibroblast growth factor (FGF) 23, a regulator of phosphate homeostasis, develop concomitant airway inflammation. Our in vitro studies point towards a similar increase of phosphate-induced inflammatory markers in human bronchial epithelial cells. FGF23 stimulation alone does not induce a proinflammatory response in the non-COPD bronchial epithelium and phosphate does not cause endogenous FGF23 release. Upregulation of the phosphate-induced proinflammatory cytokines is accompanied by activation of the extracellular-signal regulated kinase (ERK) pathway. Moreover, the addition of cigarette smoke extract (CSE) during phosphate treatments exacerbates inflammation as well as ERK activation, whereas co-treatment with FGF23 attenuates both the phosphate as well as the combined phosphate- and CS-induced inflammatory response, independent of ERK activation. Together, these data demonstrate a novel pathway that potentially explains pathological kidney-lung crosstalk with phosphate as a key mediator.
Traffic monitoring system design considering multi-hazard disaster risks
Authors: Gazzea Michele , Miraki Amir , Alisan Onur , Kuglitsch Monique M. , Pelivan Ivanka , Ozguven Eren Erman , Arghandeh Reza 
Published: March 2023

AbstractRoadways are critical infrastructure in our society, providing services for people through and between cities. However, they are prone to closures and disruptions, especially after extreme weather events like hurricanes. At the same time, traffic flow data are a fundamental type of information for any transportation system. In this paper, we tackle the problem of traffic sensor placement on roadways to address two tasks at the same time. The first task is traffic data estimation in ordinary situations, which is vital for traffic monitoring and city planning. We design a graph-based method to estimate traffic flow on roads where sensors are not present. The second one is enhanced observability of roadways in case of extreme weather events. We propose a satellite-based multi-domain risk assessment to locate roads at high risk of closures. Vegetation and flood hazards are taken into account. We formalize the problem as a search method over the network to suggest the minimum number and location of traffic sensors to place while maximizing the traffic estimation capabilities and observability of the risky areas of a city.
Design of topology optimized compliant legs for bio-inspired quadruped robots
Authors: Sun Yilun , Zong Chujun , Pancheri Felix , Chen Tong , Lueth Tim C. 
Published: March 2023

AbstractRobotic legs are an important component of the quadruped robot for achieving different motion gaits. Although the conventional rigid-link-based legs can generally perform robust motions, they still have the issues with poor sealing when operating in complex and liquid terrains. To cope with this problem, fully compliant legs with monolithic structure have been introduced in recent years to improve the system compactness and structural compliance of quadruped robots. In this article, we present a topology-optimization-based method to achieve efficient design of compliant robotic legs. In order to balance the structural stiffness and bending flexibility of the realized leg, a multi-objective optimization algorithm is utilized. A series of design cases are presented to illustrate the design principle and analytical procedure of the proposed method. In addition, experimental evaluation is also performed, and the results have demonstrated that, a quadruped robot with the optimized legs can successfully achieve stable and continuous straight-line walking motions.
Effect of ambient wind on the performance of alpine downhill skier
Authors: Li Bo , Li Peng , Zhang Yuanzhao , Jia Kun , Hong Ping 
Published: March 2023

AbstractAlpine skiing, especially alpine downhill, is one of the most extreme winter sports in terms of high-speed and narrow winning margin, and its tracks are always located in mountainous areas with high altitudes and complex ambient wind fields, resulting in a significant impact of ambient wind on the performance and the final ranking of alpine downhill skiers. In the present study, a method based upon the combination of field measurements, wind tunnel tests and kinematic simulations was used to evaluate the effect of ambient wind on the performance of an alpine downhill skier. Considering the effect of ambient wind, a kinematic model of the alpine skier-ski system was established, and the equations of motion for straight gliding and turning were deduced. Then, the Chinese National Alpine Ski Center (CNASC) downhill track was taken as a case study to investigate the effect of ambient wind on the gliding time using the proposed combined evaluation method. Field measurements and wind tunnel tests were performed to identify five critical ambient wind directions of 270°, 292.5°, 315°, 337.5° and 360°. Moreover, the wind speeds and the wind directions for 16 different measurement points of the downhill track were also obtained. The results of the modelling analysis showed that the finish time increased by 19.75% for the ambient wind direction of 270°, whereas the finish time decreased by 1.29% for the ambient wind direction of 360°.
A common variant rs2054564 in ADAMTS17 is associated with susceptibility to lumbar spondylosis
Authors: Taniguchi Yuki , Akune Toru , Nishida Nao , Omori Go , HA Kim , Ueno Kazuko , Saito Taku , Oichi Takeshi , Koike Asako , Mabuchi Akihiko , Oka Hiroyuki , Muraki Shigeyuki , Oshima Yasushi , Kawaguchi Hiroshi , Nakamura Kozo , Tokunaga Katsushi , Tanaka Sakae , Yoshimura Noriko 
Published: March 2023

AbstractThe molecular pathophysiology underlying lumbar spondylosis development remains unclear. To identify genetic factors associated with lumbar spondylosis, we conducted a genome-wide association study using 83 severe lumbar spondylosis cases and 182 healthy controls and identified 65 candidate disease-associated single nucleotide polymorphisms (SNPs). Replication analysis in 510 case and 911 control subjects from five independent Japanese cohorts identified rs2054564, located in intron 7 of ADAMTS17, as a disease-associated SNP with a genome-wide significance threshold (P = 1.17 × 10–11, odds ratio = 1.92). This association was significant even after adjustment of age, sex, and body mass index (P = 7.52 × 10–11). A replication study in a Korean cohort, including 123 case and 319 control subjects, also verified the significant association of this SNP with severe lumbar spondylosis. Immunohistochemistry revealed that fibrillin-1 (FBN1) and ADAMTS17 were co-expressed in the annulus fibrosus of intervertebral discs (IVDs). ADAMTS17 overexpression in MG63 cells promoted extracellular microfibrils biogenesis, suggesting the potential role of ADAMTS17 in IVD function through interaction with fibrillin fibers. Finally, we provided evidence of FBN1 involvement in IVD function by showing that lumbar IVDs in patients with Marfan syndrome, caused by heterozygous FBN1 gene mutation, were significantly more degenerated. We identified a common SNP variant, located in ADAMTS17, associated with susceptibility to lumbar spondylosis and demonstrated the potential role of the ADAMTS17-fibrillin network in IVDs in lumbar spondylosis development.