bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATF2-driven osteogenic activity of enoxaparin sodium-loaded polymethylmethacrylate bone cement in femoral defect regeneration
Authors: Ding Luobin , Hao Kangning , Sang Linchao , Shen Xiaoyu , Zhang Ce , Fu Dehao , Qi Xiangbei 
Published: August 2023

Abstract
Background
Polymethylmethacrylate (PMMA) bone cement loaded with enoxaparin sodium (PMMA@ES) has been increasingly highlighted to affect the bone repair of bone defects, but the molecular mechanisms remain unclear. We addressed this issue by identifying possible molecular mechanisms of PMMA@ES involved in femoral defect regeneration based on bioinformatics analysis and network pharmacology analysis.

Methods
The upregulated genes affecting the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) were selected through bioinformatics analysis, followed by intersection with the genes of ES-induced differentiation of BMSCs identified by network pharmacology analysis. PMMA@ES was constructed. Rat primary BMSCs were isolated and cultured in vitro in the proliferation medium (PM) and osteogenic medium (OM) to measure alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and the expression of RUNX2 and OCN using gain- or loss-of-function experiments. A rat femoral bone defect model was constructed to detect the new bone formation in rats.

Results
ATF2 may be a key gene in differentiating BMSCs into osteoblasts. In vitro cell assays showed that PMMA@ES promoted the osteogenic differentiation of BMSCs by increasing ALP activity, extracellular matrix mineralization, and RUNX2 and OCN expression in PM and OM. In addition, ATF2 activated the transcription of miR-335-5p to target ERK1/2 and downregulate the expression of ERK1/2. PMMA@ES induced femoral defect regeneration and the repair of femoral defects in rats by regulating the ATF2/miR-335-5p/ERK1/2 axis.

Conclusion
The evidence provided by our study highlighted the ATF2-mediated mechanism of PMMA@ES in the facilitation of the osteogenic differentiation of BMSCs and femoral defect regeneration.
Active versus passive distraction for reducing procedural pain and anxiety in children: a meta-analysis and systematic review
Authors: Shen Ting , Wang Xixi , Xue Qiaoyun , Chen Dan 
Published: August 2023

Abstract
Background
Procedural pain is very important in clinical children care. We aimed to evaluate the effects of active versus passive distraction for reducing procedural pain and anxiety in children.

Methods
Two researchers searched the Web of Science, PubMed, EMBASE, Cochrane, SinoMed, Wanfang, China National Knowledge Infrastructure, Weipu databases for the randomized controlled trials(RCTs) on the active versus passive distraction affecting procedural pain and anxiety in children until May 18, 2023. The literature screening and data extraction were carried out by two researchers independently. Review Manager 5.3 software was used for data analysis.

Results
13 RCTs were finally included. 553 children received active distraction intervention and 551 children received passive distraction intervention. There were no significant differences in the children self-reported procedural pain betweent active and passive distraction. The parent-reported procedural pain, medical staff-reported procedural pain, children-reported procedural anxiety, parent-reported procedural anxiety, medical staff-reported procedural anxiety in the active distraction were significant less than that of active distraction. Egger regression analysis showed that there was no publication bias in the results.

Conclusions
Existing evidence suggests that active distraction may be more effective in reducing operational pain and anxiety in children than passive distraction. More studies on the effects of active distraction versus passive distraction in children with larger sample size are needed in the future.
Wolbachia wMel strain-mediated effects on dengue virus vertical transmission from Aedes aegypti to their offspring
Authors: Duong Thi Hue Kien , da Silva Goncalves Daniela , Tran Thuy Vi , Thi Vo Long , Le Thi Dui , Vu Tuyet Nhu , Nguyen Thi Giang , Huynh Thi Xuan Trang , Nguyen Minh Nguyet , Nguyen Thanh Phong , Yacoub Sophie , Simmons Cameron P. 
Published: August 2023

Abstract
Background
Dengue virus serotypes (DENV-1 to -4) can be transmitted vertically in Aedes aegpti mosquitoes. Whether infection with the wMel strain of the endosymbiont Wolbachia can reduce the incidence of vertical transmission of DENV from infected females to their offspring is not well understood.

Methods
A laboratory colony of Vietnamese Ae. aegypti, both with and without wMel infection, were infected with DENV-1 by intrathoracic injection (IT) to estimate the rate of vertical transmission (VT) of the virus. VT in the DENV-infected mosquitoes was calculated via the infection rate estimation from mosquito pool data using maximum likelihood estimation (MLE).

Results
In 6047 F1 Vietnamese wild-type Ae. aegypti, the MLE of DENV-1 infection was 1.49 per 1000 mosquitoes (95% confidence interval [CI] 0.73–2.74). In 5500 wMel-infected Ae. aegypti, the MLE infection rate was 0 (95% CI 0–0.69). The VT rates between mosquito lines showed a statistically significant difference.

Conclusions
The results reinforce the view that VT is a rare event in wild-type mosquitoes and that infection with wMel is effective in reducing VT.

Graphical Abstract
Recent increase in low complexity polygenomic infections and sialic acid-independent invasion pathways in Plasmodium falciparum from Western Gambia
Authors: Nganyewo Nora Nghochuzie , Bojang Fatoumata , Oriero Eniyou Cheryll , Drammeh Ndey Fatou , Ajibola Olumide , Mbye Haddijatou , Jawara Aminata Seedy , Corea Simon , Awandare Gordon Akanzuwine , D’Alessandro Umberto , Amenga-Etego Lucas N. , Amambua-Ngwa Alfred 
Published: August 2023

AbstractBackgroundThe malaria parasitePlasmodium falciparumutilizes multiple alternative receptor-ligand interactions for the invasion of human erythrocytes. While someP. falciparumclones make use of sialic acid (SA) residues on the surface of the human glycophorin receptors to invade the erythrocyte, others use alternative receptors independent of sialic acid residues. We hypothesized that over the years, intensified malaria control interventions and declining prevalence in The Gambia have resulted in a selection of parasites with a dominant invasion pathways and ligand expression profiles.MethodsBlood samples were collected from 65 malaria-infected participants with uncomplicated malaria across 3 years (2015, 2016, and 2021). Genetic diversity was determined by genotyping the merozoite surface protein 2 (msp2) polymorphic gene ofP. falciparum.Erythrocyte invasion phenotypes were determined using neuraminidase, trypsin, and chymotrypsin enzymes, known to cleave different receptors from the surface of the erythrocyte. Schizont-stage transcript levels were obtained for a panel of 6P. falciparuminvasion ligand genes (eba175, eba181, Rh2b, Rh4, Rh5, andclag2) using 48 successfully cultured isolates.ResultsThough the allelic heterozygosity ofmsp2repeat region decreased as expected with reduced transmission, there was an increase in infections with more than a singlemsp2allelotype from 2015 to 2021. The invasion phenotypes of these isolates were mostly SA independent with a continuous increase from 2015 to 2021. Isolates from 2021 were highly inhibited by chymotrypsin treatment compared to isolates from 2015 and 2016. Higher invasion inhibition for 2021 isolates was further obtained following erythrocyte treatment with a combination of chymotrypsin and trypsin. The transcript levels of invasion ligand genes varied across years. However, levels ofclag2, a rhoptry-associated protein, were higher in 2015 and 2016 isolates than in 2021 isolates, whileRh5levels were higher in 2021 compared to other years.ConclusionsOverall, these findings suggest increasing mixed infections with an increase in the use of sialic-acid independent invasion pathways byP. falciparumclinical isolates in the Western part of Gambia.Graphical Abstract
Fathers’ preconception smoking and offspring DNA methylation
Authors: Kitaba Negusse Tadesse , Knudsen Gerd Toril Mørkve , Johannessen Ane , Rezwan Faisal I. , Malinovschi Andrei , Oudin Anna , Benediktsdottir Bryndis , Martino David , González Francisco Javier Callejas , Gómez Leopoldo Palacios , Holm Mathias , Jõgi Nils Oskar , Dharmage Shyamali C. , Skulstad Svein Magne , Watkins Sarah H. , Suderman Matthew , Gómez-Real Francisco , Schlünssen Vivi , Svanes Cecilie , Holloway John W. 
Published: August 2023

AbstractBackgroundExperimental studies suggest that exposures may impact respiratory health across generations via epigenetic changes transmitted specifically through male germ cells. Studies in humans are, however, limited. We aim to identify epigenetic marks in offspring associated with father’s preconception smoking.MethodsWe conducted epigenome-wide association studies (EWAS) in the RHINESSA cohort (7–50 years) on father’s any preconception smoking (n = 875 offspring) and father’s pubertal onset smoking < 15 years (n = 304), using Infinium MethylationEPIC Beadchip arrays, adjusting for offspring age, own smoking and maternal smoking. EWAS of maternal and offspring personal smoking were performed for comparison. Father’s smoking-associated dmCpGs were checked in subpopulations of offspring who reported no personal smoking and no maternal smoking exposure.ResultsFather’s smoking commencing preconception was associated with methylation of blood DNA in offspring at two cytosine-phosphate-guanine sites (CpGs) (false discovery rate (FDR) < 0.05) inPRR5andCENPP. Father’s pubertal onset smoking was associated with 19 CpGs (FDR < 0.05) mapped to 14 genes (TLR9,DNTT,FAM53B,NCAPG2,PSTPIP2,MBIP,C2orf39,NTRK2,DNAJC14,CDO1,PRAP1,TPCN1,IRS1andCSF1R). These differentially methylated sites were hypermethylated and associated with promoter regions capable of gene silencing. Some of these sites were associated with offspring outcomes in this cohort including ever-asthma (NTRK2), ever-wheezing (DNAJC14, TPCN1), weight (FAM53B, NTRK2) and BMI (FAM53B, NTRK2) (p < 0.05). Pathway analysis showed enrichment for gene ontology pathways including regulation of gene expression, inflammation and innate immune responses. Father’s smoking-associated sites did not overlap with dmCpGs identified in EWAS of personal and maternal smoking (FDR < 0.05), and all sites remained significant (p < 0.05) in analyses of offspring with no personal smoking and no maternal smoking exposure.ConclusionFather’s preconception smoking, particularly in puberty, is associated with offspring DNA methylation, providing evidence that epigenetic mechanisms may underlie epidemiological observations that pubertal paternal smoking increases risk of offspring asthma, low lung function and obesity.
Engineered clinical-grade mesenchymal stromal cells combating SARS-CoV-2 omicron variants by secreting effective neutralizing antibodies
Authors: Wang Yanning , Gao Tianyun , Li WanTing , Tai Chenxu , Xie Yuanyuan , Chen Dong , Liu Shuo , Huang Feifei , Wang Wenqing , Chen Yuxin , Wang Bin 
Published: August 2023

Abstract
Background
The emergence of SARS-CoV-2 becomes life-threatening for the older and immunocompromised individuals, whereas limited treatment is available on these populations. Mesenchymal stromal cells (MSCs) have been reported to be useful in SARS-CoV-2 treatment and reduce SARS-CoV-2-related sequelae.

Results
In this study, we developed an autonomous cellular machine to secret neutralizing antibody in vivo constantly based on the clinical-grade MSCs, to combat SARS-CoV-2 infections. First, various modified recombinant plasmids were constructed and transfected into clinical-grade MSCs by electroporation, for assembly and expression of neutralizing anti-SARS-CoV-2 antibodies. Second, the stable antibody secreting MSCs clones were screened through pseudovirus neutralization assay. Finally, we investigated the pharmacokinetics and biodistribution of neutralizing antibody secreted by engineered MSCs in vivo. The stable clinical-grade MSCs clones, expressing XGv347-10 and LY-CoV1404-5 neutralizing antibodies, exhibited their feasibility and protective efficacy against SARS-CoV-2 infection. Transplanted engineered clinical-grade MSCs effectively delivered the SARS-CoV-2 antibodies to the lung, and the immune hyperresponsiveness caused by COVID-19 was coordinated by MSC clones through inhibiting the differentiation of CD4 + T cells into Th1 and Th17 subpopulations.

Conclusions
Our data suggested that engineered clinical-grade MSCs secreting effective neutralizing antibodies as cellular production machines had the potential to combat SARS-CoV-2 infection, which provided a new avenue for effectively treating the older and immunocompromised COVID-19 patients.
Diagnostic yield, safety and therapeutic consequences of myocardial biopsy in clinically suspected fulminant myocarditis unweanable from mechanical circulatory support
Authors: Marquet Yann , Hékimian Guillaume , Lebreton Guillaume , Kerneis Mathieu , Rouvier Philippe , Bay Pierre , Mathian Alexis , Bréchot Nicolas , Chommeloux Juliette , Petit Matthieu , Gautier Melchior , Lefevre Lucie , Saura Ouriel , Levy David , Quentric Paul , Moyon Quentin , Ortuno Sofia , Schmidt Matthieu , Leprince Pascal , Luyt Charles-Edouard , Combes Alain , Pineton de Chambrun Marc 
Published: August 2023

Abstract
Background
Fulminant myocarditis is a rare and severe disease whose definite and etiological diagnoses rely on pathological examination. Albeit, myocardial biopsy can be associated with significant morbidity and mortality, its therapeutic consequences are unclear. We conducted a study to determine the diagnostic yield, the safety and the therapeutic consequences of myocardial biopsy in patients with fulminant clinically suspected myocarditis unweanable from mechanical circulatory support (MCS).

Methods
Monocenter, retrospective, observational cohort study in a 26-bed French tertiary ICU between January 2002 and February 2019. Inclusion of all fulminant clinically suspected myocarditis patients undergoing in-ICU myocardial biopsy while being on MCS. The primary endpoint was the proportion of patients classified as definite myocarditis using Bonaca criteria before and after including myocardial biopsy results.

Results
Forty-seven patients (median age 41 [30–47], female 53%) were included: 55% died before hospital discharge, 34% could be bridged-to-recovery and 15% bridged-to-transplant. Myocardial biopsy was endomyocardial or surgical in 36% and 64% cases respectively. Tamponade requiring emergency pericardiocentesis occurred in 29% patients after endomyocardial biopsy. After adding the biopsy results in the Bonaca classification algorithm the percentage of definite myocarditis raised from 13 to 55% (p < 0.0001). The rate of biopsy-related treatments modifications was 13%, leading to patients’ recovery in only 4% patients.

Conclusions
In clinically suspected myocarditis unweanable from MCS, myocardial biopsy increased the rate of definite myocarditis but was associated with a low rate of treatment modification and a significant proportion of adverse events. We believe the benefit/risk ratio of myocardial biopsy should be more carefully weighted in these frail and selected patients than suggested by actual guidelines. Further prospective studies are now needed to determine its value in patients under MCS.

Graphical Abstract
Multiple solutions for a class of anisotropic p⃗-Laplacian problems
Authors: Bonanno G. , D’Aguì G. , Sciammetta A. 
Published: August 2023

AbstractIn this paper we present some existence and multiplicity results for a class of anisotropic p⃗-Laplacian problems with Dirichlet boundary conditions. In particular, the existence of three solutions is pointed out. The approach is based on variational methods and our main tool is a three critical point theorem.
The association between Urban and Rural Resident Basic Medical Insurance and instrumental activity of daily living disability among middle-aged and older adults in China
Authors: Sun Jian , Yu Shui , Lu Wanjun , Liu Yujiang 
Published: August 2023

AbstractBackgroundPrevious studies have not investigated the association between medical insurance and instrumental activity of daily living (IADL) disability. To fulfill this research gap, this study aims to explore the association between Urban and Rural Resident Basic Medical Insurance (URRBMI) and IADL disability among middle-aged and older adults in China.MethodsThe data of this study were sourced from the 2018 wave of China Health and Retirement Longitudinal Study (CHARLS). Logit regression models were used to analyze the association between URRBMI and odds of suffering from IADL disability. Furthermore, we used IV-Probit regression model to address the potential endogeneity problem. Moreover, propensity score matching and generalized random forest model were employed to conduct robustness checks.ResultsThe logit regression results reveal that URRBMI participation was significantly related to reduced odds of suffering from IADL disability by 39.86% after adjusting for the control variables (p < 0.01). The results of IV-Probit estimation show that URRBMI was an exogenous variable. Further robustness checks reported similar estimation results. The results of heterogeneity analysis reveal that URRBMI produced a statistically stronger effect on IADL disability for the older adults (OR = 0.5815,p < 0.01) when compared with the middle-aged adults (OR = 0.5690,p < 0.05). The results of impact channel analysis indicate that physical exercise was a channel involving the effect of URRBMI on IADL disability.ConclusionThis study finds that the middle-aged and older adults who were covered by URRBMI had a reduced possibility of suffering from IADL disability when compared with those without URRBMI. Furthermore, it is found that URRBMI produced a statistically stronger effect on IADL disability for the older adults when compared with the middle-aged adults. Moreover, we obtain evidence indicating that physical exercise was a channel involving the effect of URRBMI on IADL disability.
Consumers' preferences for processed meat: a best–worst scaling approach in three European countries
Authors: Török Áron , Yeh Ching-Hua , Menozzi Davide , Balogh Péter , Czine Péter 
Published: August 2023

AbstractProcessed meat products are a staple part of the typical European diet. Product packaging can include a considerable amount of information and, with other intrinsic and extrinsic attributes, substantially influence consumers' preferences and purchasing decisions. This study investigates 14 product attributes of processed meat products using a cross-country analysis. Based on an online survey conducted in Hungary (n = 410), Italy (n = 268), and Serbia (n = 402), an object-case best–worst scaling approach was applied. Results reveal both international and country-specific characteristics of preferences. Best-Worst scores reveal that taste and best-before date are among the most significant considerations in all three countries, while brand is among the attributes considered least important. Comparisons indicate significant differences according to country and socioeconomic characteristics. The study provides managerial implications.
Predicting moose behaviors from tri-axial accelerometer data using a supervised classification algorithm
Authors: Kirchner Theresa M. , Devineau Olivier , Chimienti Marianna , Thompson Daniel P. , Crouse John , Evans Alina L. , Zimmermann Barbara , Eriksen Ane 
Published: August 2023

Abstract
Background
Monitoring the behavior of wild animals in situ can improve our understanding of how their behavior is related to their habitat and affected by disturbances and changes in their environment. Moose (Alces alces) are keystone species in their boreal habitats, where they are facing environmental changes and disturbances from human activities. How these potential stressors can impact individuals and populations is unclear, in part due to our limited knowledge of the physiology and behavior of moose and how individuals can compensate for stress and disturbances they experience. We collected data from collar-mounted fine-scale tri-axial accelerometers deployed on captive moose in combination with detailed behavioral observations to train a random forest supervised classification algorithm to classify moose accelerometer data into discrete behaviors. To investigate the generalizability of our model to collared new individuals, we quantified the variation in classification performance among individuals.

Results
Our machine learning model successfully classified 3-s accelerometer data intervals from 12 Alaskan moose (A. a. gigas) and two European moose (A. a. alces) into seven behaviors comprising 97.6% of the 395 h of behavioral observations conducted in summer, fall and spring. Classification performance varied among behaviors and individuals and was generally dependent on sample size. Classification performance was highest for the most common behaviors lying with the head elevated, ruminating and foraging (precision and recall across all individuals between 0.74 and 0.90) comprising 79% of our data, and lower and more variable among individuals for the four less common behaviors lying with head down or tucked, standing, walking and running (precision and recall across all individuals between 0.28 and 0.79) comprising 21% of our data.

Conclusions
We demonstrate the use of animal-borne accelerometer data to distinguish among seven main behaviors of captive moose and discuss generalizability of the results to individuals in the wild. Our results can support future efforts to investigate the detailed behavior of collared wild moose, for example in the context of disturbance responses, time budgets and behavior-specific habitat selection.
Testing the effects of passive integrated transponder (PIT) tags on survival, growth, and tag retention of common nase (Chondrostoma nasus L.) and European barbel (Barbus barbus L.)
Authors: Nagel Christoffer , Droll Jan , Kroemer Katharina , Pander Joachim , Geist Juergen 
Published: August 2023

AbstractFreshwater fish populations are in steep decline, prompting conservation measures and a need for their evaluation. Fish are increasingly monitored with passive integrated transponders (PIT), although the suitability of this tagging technique has not yet been validated for most European target species of conservation. Consequently, this study tested the effect of commonly used 12 mm full-duplex (FDX) PIT tags implanted into the abdominal cavity of common nase (Chondrostoma nasus L.) and European barbel (Barbus barbus L.). A controlled laboratory setup was used to compare survival, growth (both length and weight) and tag retention for two different size classes of sub-adults over 61 days. Survival in the treatment groups was high (96.7–100%) and not statistically different from the control groups (97.5–100%). Highest mortality occurred in small tagged barbel (n = 4; 96.7% survival), while no mortality occurred in large tagged nase. Mean growth rates for tagged fish (2.28 mm, 3.26 g) were similar to those of control fish (2.77 mm, 3.59 g). Overall tag retention rate was 99.0% and tag loss only occurred in nase. The results of this study demonstrate the suitability of PIT injection in the body cavity of nase and barbel > 100 mm TL, which is of high methodological importance given the increasing role these species play in PIT tag-based assessments of freshwater fish conservation in European rivers.