bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors
Authors: Gruber Rosalie , Ludewig María Verónica Montilva , Weßels Christina , Schlang Gerlinde , Jedhoff Svenja , Herbrandt Swetlana , Mattner Frauke 
Published: May 2023

Abstract

PurposeTo evaluate the frequency, duration and patterns of long-term coronavirus disease 2019 (COVID-19) symptoms and to analyse risk factors for long-lasting COVID-19 sequelae among a cohort of hospital employees (HEs).MethodsWe conducted a survey regarding persistent COVID-19 related symptoms with all HEs from three medical centres in Cologne, Germany, who were tested SARS-CoV-2 PCR positive from March 2020 until May 2021. Duration of symptoms and possible risk factors for protracted COVID-19 course were analysed.ResultsOf 221 included HEs, a number of 104 HEs (47.1%) reported at least one persisting symptom for more than 90 days after initial SARS-CoV-2 detection. Each one cycle higher initial Ct value significantly increased the chances of overcoming symptoms (odds ratio [OR] = 1.05; p = 0.019). The occurrence of breathlessness within the first ten days (OR = 7.89; p = 0.008), an initial Ct value under 30 (OR = 3.36; p = 0.022) and a definitely nosocomial SARS-CoV-2 transmission (OR = 3.05; p = 0.049) showed a statistically significant association with increased odds of illness duration over 90 days.ConclusionAbout half of the HEs suffered from long lasting symptoms over 90 days after almost entirely mild acute COVID-19. Predictive factors could possibly be used for early treatment to prevent development of long-term symptoms after COVID-19 in future.
CVID-associated intestinal disorders in the USIDNET registry: An analysis of disease manifestations, functional status, comorbidities, and treatment
Authors: Franzblau Lauren E. , Fuleihan Ramsay L. , Cunningham-Rundles Charlotte , Wysocki Christian A. 
Published: May 2023

Abstract
Common variable immunodeficiency (CVID) has been subdivided into five phenotypes, including one marked by non-infectious enteropathies that lead to significant morbidity and mortality. We examined a large national registry of patients with CVID to better characterize this population and understand how the presence of enteropathy influences nutritional status, patient function, and the risk of additional non-infectious disorders in CVID patients. We also sought to illustrate the range of treatment strategies for CVID-associated enteropathies. We extracted patient data from the United States Immunodeficiency Network (USIDNET) database, which included 1415 patients with CVID, and compared those with and without intestinal disorders. Demographic and genetic profiles, functional status, and treatments targeting intestinal disorders are reported. Intestinal disorders were present in 20% of patients with CVID, including chronic diarrhea, inflammatory bowel disease, malabsorption, and others. Compared to those without enteropathies, this patient subset exhibited significantly lower Karnofsky-Lansky functional scores, greater reliance on nutritional support, higher rates of vitamin deficiencies, and increased prevalence of hematologic disorders, liver disease, pulmonary disease, granulomatous disease, and lymphoma. Genetic data were reported for only 5% of the cohort. No mutations segregated significantly to patients with or without intestinal disease. Corticosteroids were most frequently used for treatment. Patients with CVID-associated intestinal disorders exhibit higher rates of autoimmune and inflammatory comorbidities, lymphoma, malnutrition, and debility. We review recent studies implicating specific pathways underlying this immune dysregulation. Further studies are needed to evaluate the role of targeted immunomodulatory therapies for CVID-associated intestinal disorders.
Construction of a F 1 DH population and high-density genetic map of ornamental kale
Authors: Guo Ning , Han Shuo , Zong Mei , Wang Guixiang , Duan Mengmeng , Liu Fan 
Published: May 2023

Abstract
Background Ornamental kale (Brassica oleracea var. acephala) is an attractive ornamental plant with a range of leaf colors and shapes. Owing to the long breeding cycle and limited availability of genetic markers, the breeding of new varieties of ornamental kale by traditional and marker-assisted selection (MAS) breeding methods is difficult. Microspore culture is an effective approach to generate new materials and a doubled-haploid (DH) population, which is suitable for genetic map construction. Genetic maps are a prerequisite for quantitative trait loci analysis, MAS, fine gene mapping, and genome sequence assembly. Here, we report the construction of a F1-derived doubled haploid (F1DH) population and high-density genetic map for ornamental kale to provide novel resources to use in breeding new varieties.Results A F1DH ornamental kale population comprising 300 DH lines, derived from parents that differed markedly in leaf shape and color, was constructed using microspore culture. More than 1000 regenerated plants were obtained, showing that the F1 progeny possessed high regeneration capacity, and the proportion of spontaneous chromosome doubling was higher than 50%. The F1DH population contained rich phenotypic diversity, especially variation in leaf color and shape. A high-density genetic map was generated by whole-genome resequencing using 150 F1DH individuals selected from the F1DH population. The genetic map contained 1696 bin-markers with 982,642 single-nucleotide polymorphisms (SNPs) spanning a total distance of 775.81 cM on all nine chromosomes with an average distance between markers of 0.46 cM. The ornamental kale genetic map contained substantially more SNP markers compared with published genetic maps for other B. oleracea crops. The haplotype and heat maps as well as the collinearity of the genetic and physical maps illustrated that the genetic map for ornamental kale was accurate and of high quality.Conclusions The F1DH progenies provide an excellent resource for germplasm innovation and breeding new varieties of ornamental kale. The high-density genetic map provides useful information for gene mapping and elucidation of the molecular mechanism of important agronomic traits of ornamental kale, and will aid in pseudochromosome assembly in genomic research on B. oleracea.
Derlin-1 ameliorates nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1
Authors: You Hui , Wen Xin , Wang Xingchun , Zhu Cuiling , Chen Haibing , Bu Le , Zhang Jun , Qu Shen 
Published: May 2023

Abstract

BackgroundThe functions of liver fatty acid binding protein (FABP1) in the regulation of nonalcoholic fatty liver disease (NAFLD) have been demonstrated. However, it is not fully understood how FABP1 expression is dynamically regulated in metabolic disorders. Previous studies have reported that ubiquitin proteasome-mediated degradation of FABP1 is involved, but the mechanism remains unknown.MethodsDysregulated expression of hepatic FABP1 and Derlin-1 was observed in NAFLD patients. We performed mice hepatic tissue co-immunoprecipitation (IP)-based mass spectrum (MS) assays. Derlin-1 interacts with FABP1 and modifies its ubiquitin status, as confirmed by co-IP. The role of Derlin-1 in lipid deposition was tested using adenovirus-mediated overexpression in C57 mice, Derlin-1 overexpression (Derlin-1-OE), or Derlin-1 knockdown (Derlin-1-KO) HepG2 cells.ResultsAs a subunit of the endoplasmic reticulum-associated degradation complex (ERAD), Derlin-1 is negatively associated with NAFLD patients and interacts with and ubiquitinates FABP1. Derlin-1 suppresses FABP1 protein levels and inhibits lipid deposition through a FABP1-dependent pathway. Additionally, Trim25, an E3 ubiquitin ligase present in the endoplasmic reticulum (ER), is recruited to promote Derlin-1-related polyubiquitylation of FABP1, thereby creating a ubiquitin-associated network for FABP1. Overexpression of Derlin-1 ameliorates hepatic steatosis in both C57 mice and HepG2 cells, and contributes to attenuated weight gain, lower liver weight, and visceral fat mass.ConclusionsFABP1, a master enzyme that maintains fatty acid metabolism, undergoes degradation by Derlin-1 through ubiquitin modification. The activation of Derlin-1 in vivo may represent a potential therapeutic strategy for NAFLD.Trial registration:Clinical Trials. gov ID: NCT02118376.
Design and experimental study of a stepped magnetorheological damper with power generation
Authors: Yang Xiaolong , Chen Yingjie , Geng Xiumei , Liu Haiyan 
Published: May 2023

AbstractTraditional vehicle suspension magnetorheological dampers have problems with low output damping force and require additional energy input to operate, to improve the performance of the vehicle suspension magnetorheological damper, in this paper we propose and investigate a stepped magnetorheological damper structure with power generation, and conducts structural design and magnetic circuit analysis. The effects of different currents, damping gaps, coil slot positions, and coil turns on the damping performance of the stepped magnetorheological damper with power generation are numerically studied. The magnetic circuit sensitivity analysis of the power generation structure and the magnetorheological damper structure is also performed. Experiments have verified the effects of different input excitations on damping and energy-feeding performance, and the results of numerical analysis have been verified. The results show that when the excitation coil is wound for 257 turns, the magnetic circuit requirements are met, and the maximum damping force of the proposed electromagnetic energy fed stepped magnetorheological damper can reach over 4500N, meeting the use requirements.
Keeping Up With The Cardashians: A report on the imbalance between car and Public transportation use during peak hours in Iligan city, Philippines
Authors: Montemor Bernard Louis , Samar Christine Rose , Tantao Nabel 
Published: May 2023

Abstract
During peak hours, traffic in the Philippines may be extremely severe and congested, particularly in big areas such as Metro Manila. This is due to a number of causes, including a large number of automobiles on the road, limited road capacity, and insufficient public transportation networks. It is the same situation in Iligan, despite the city being just a fraction of what Metro Manila is. In this data note, we report a massive imbalance between car and public transport use, especially in peak hours where large volumes of traffic pass through the main arterial road networks, and what this imbalance implies on the current state of the mass transportation system of Iligan city.
The Association between Domestic Violence and Adverse Pregnancy Outcomes in Pregnant Women Seeking Antenatal Care at Public Hospitals in Gedeo Zone: A Prospective Cohort Study
Authors: Ayele Getnet Melaku , Abebe Mesfin , Mulat Seid Shumye , Kebede Selamawit Semagn , Getahun Senait Belay 
Published: May 2023

Abstract
Background: Domestic violence is any harmful behavior by an intimate partner, such as physical, sexual, or psychological abuse. In Ethiopia, domestic violence is a serious and widespread problem. It affects nearly two-thirds (64.6%) of pregnant women and increases the risk of maternal and perinatal morbidity and mortality. Domestic violence during pregnancy is a growing public health problem that may contribute to maternal and perinatal mortality, especially in low and middle income countries. This study aims to assess the association between domestic violence during pregnancy and the risk of adverse pregnancy outcomes in Gedeo zone Public Hospitals, Southern Ethiopia.
Material and Methods: We conducted a prospective cohort study on 142 pregnant women in their third trimester who attended public health institutions in Gedeo Zone for antenatal care. We compared 47 women who were exposed to domestic violence with 95 who were not and followed them until 24 hours after delivery or drop-out. We used SPSS version-24 to analyze data and logistic regression to examine the association between domestic violence and pregnancy outcomes. We reported the results using an adjusted odds ratio with 95% confidence interval and P-value.
Result: Out of 142 women, who completed the follow-up, 47 were exposed to domestic violence and 95 were not. We found a strong link between domestic violence and preterm birth. Women exposed to domestic violence had a four-fold higher risk of preterm birth (AOR= 4.392, 95%CI: 1.117, 6.588) than those who were not. They also had a 2.5-fold higher risk of perinatal death (AOR= 2.562, 95%CI: 1.041, 6.308).
Conclusion: Domestic violence during pregnancy affects many pregnant women in southern Ethiopia and harms their babies. It leads to preterm birth and perinatal death, which can be prevented. The Ethiopian government and other stakeholders need to protect pregnant women from intimate partner violence urgently.
Molecular Docking and Dynamics Simulation revealed the Potential Inhibitory Activity of Piomozide against Kynurenine metabolism targeting the Kynurenine 3-monooxygenase
Authors: Kaladan Kavya Vinil , Sivagurunathan Narmadhaa , Parsanathan Rajesh 
Published: May 2023

Abstract
L-tryptophan metabolism depends on the enzyme kynurenine 3-monooxygenase (KMO). Quinolinic acid, kynurenic acid, and other metabolites having consequences for neurology and psychiatry are mostly derived from L-tryptophan. Furthermore, it was shown that hypertension causes an increase in KMO; hence, inhibiting KMO may aid in preventing both hypertension and cardiovascular diseases. In the current investigation, we used in silico techniques to screen for potential KMO inhibitors. SWISS-MODEL was utilized to simulate the homology with full-length rat KMO complexes with a pyrazoyl benzoic acid inhibitor (PDB:6LKD; sequence identity 80.17%) because the previously available human KMO structure (PDB:5X68) was broken. The flexibility of protein structures was predicted using CABS-flex2.0, and Qmean, Errat, and Procheck were used to improve and validate the 3D models. We used receptor-based screening and drug repurposing. The DrugRep virtual screening server was used for high-throughput docking with FDA-approved, FDA-experimental, and traditional Chinese medicine libraries. The lead-like compounds with good pharmacokinetics characteristics, which may have superior stability and affinity for KMO, were discovered to be the top-scoring drugs from the libraries. Pimozide, an approved pharmaceutical, establishes Van der Waals interactions at ARG85 and TYR99, as well as at TYR398 (4.77Å) and ASN363 (3.16Å) for Pi-Hydrophobic and Halogen Fluorine respectively, all of which are important L-kynurenine binding sites. The co-factor FAD binding nearest residue SER53 formed a hydrogen bond interaction (2.89Å). A molecular dynamic simulation of the FDA-approved drug pimozide exhibited greater interactions and affinities for KMO, which may be able to successfully target the KMO. Pimozide is also used as therapy to treat a variety of psychotic effects, neurodegenerative diseases, and hypertension.
New report of endophytic bacterium Achromobacter xylosoxidans from root tissue of Musa paradisiaca
Authors: Chowhan Papan , Chakraborty Arka Pratim 
Published: May 2023

Abstract
Banana plant (Musa paradisiaca) worldwide cultivated crop harbors many endophytic bacteria. Endophytic bacterium (MRH 11) isolated from root tissue of M. paradisiaca was identified as Achromobacter xylosoxidans (ON955872) which showed positive response in IAA production, phosphate solubilization, catalase production. A. xylosoxidans also showed in vitro antagonism against Curvularia lunata causing leaf spot disease of banana.
Overlapping and Distinct Functions of an Extended Repertoire of KRAS Mutations
Authors: Zimmermann Michael , Mathison Angela , Li Xuan , Leverence Elise , Jensen Davin , Esparza Guadalupe , De Assuncao Thiago Milech , Smith Brian , Volkman Brian , Lomberk Gwen , Urrutia Raul 
Published: May 2023

Abstract

The challenge of interpreting novel genetic variations in genomics is widespread. Previous studies have shown the role of KRAS mutations in heritable and somatic conditions, but more genetic variants have been observed than have been characterized. This study compares a broad genomic landscape of 25 KRAS mutations at three cellular checkpoint levels: ERK and AKT signaling, early cell-intrinsic transcriptional regulation, and secretory inflammatory responses. The results show heterogeneity in KRAS biology, with distinct mutations inducing significantly different levels of phosphorylated ERK, transcriptional profiles, and cytokine protein profiles. This heterogeneity may underlie variation in inter-individual disease patterns and contribute to differences in disease initiation or progression. Transcriptional profiles implicate different extents of feedback from upstream receptor kinases through diverse downstream nuclear targets and immunomodulatory programs. Integrated analysis reveals heterogeneity and potentially actionable outcomes for distinct groups of KRAS mutations in human cells.
First record of mussel larvae occurring in the mantle cavity of littoral-dwelling Mytilus spp. in Svalbard, Arctic
Authors: Olenin Sergej , Minchin Dan , Samuilovienė Aurelija , Kotwicki Lech , Węsławski Jan Marcin 
Published: May 2023

Abstract
After a millennium, the blue mussels Mytilus have returned to the high Arctic region of Svalbard in the beginning of 21st century. Later it was found that not only M. edulis arrived in Svalbard, but a whole complex of sympatric species including M. edulis, M. galloprovincialis, M. trossulus and their hybrids, which may have a wide range of origins from both sides of the North Atlantic. The mussels, which first were found in the sublittoral at the mouth of Isfjorden on the west coast of Spitsbergen island, now are spreading in other areas, and recently were also found in the intertidal zone. Here we report on the finding of viable larvae at the late stages of development (up to pediveliger) that were recovered from the mantle cavity of mussels collected from the lower intertidal shore in late July 2019 and 2021 in Trygghamna, Isfjorden, Spitsbergen. Both the identity of the adult mussels and the larvae was confirmed using by sequencing of barcode region. Larvae ranged in size from 178 to 376 µm, averaging 295 ± 35 µm. They were recovered from mussel specimens ranging from 21 to 57 mm in length, and their ages were estimated, based on the number of rings, from 3 to 12 years. It remains unclear whether the retention of larvae within the mantle cavity results from the intake of near shore concentrations and whether it leads to larviphagy.
Morphotectonic development of surface karst in Western Taurus (Türkiye)
Authors: Şener Mehmet Furkan , Şimşek Mesut , Utlu Mustafa , Öztürk Muhammed Zeynel , Sözbilir Hasan 
Published: May 2023

Abstract

The Taurus Mountains include the most important karstic terrain of Turkey and are divided into three parts the Western, Central, and Eastern Taurus. These mountains include many surface and subsurface karst landforms and the morphometric features of these landforms provide important data on the geomorphological and morphotectonic development of karst areas in the Taurus Mountains. Karstic depressions (poljes and dolines) constitute the most important surface landforms in the Western Taurus Mountains. In this study, a total of 7093 micro depressions (doline) and 74 macro depressions (polje) were detected and morphometric properties were calculated located in the Western Taurus Mountains. The poljes developed within the Beydağları Autochthon and Lycian nappes, while the dolines developed especially in the high plateaus within the Beydağları Autochthon. The morphotectonic development of both landforms is as below shortly. As a result of the northward movement of the African plate from the Early Cretaceous to the Late Miocene, the limestones accumulated in the shallow marine environment were compressed in the north direction and made a counterclockwise movement. Depending on this tectonic activity, the Lycian Nappes from the northwest and the Antalya Nappes from the east thrust over the Baydağları autochthon, and thus nappes, reverse faults and fold systems developed in the study area. The Western Taurus began to continentalization from the Oligocene and began to erode from the Miocene, and also with the erosion, the valley systems began to develop in the Western Taurus. The NE-SW and NW-SE directional normal faults have developed under the influence of extensional tectonics since the Miocene, and this extensional tectonism has caused widespread development of the poljes in the study area. In addition, dolines and paleovalley began to develop in limestone areas at higher elevations with the lowering of the karst base level. All morphometric and morphotectonic processes reveal that the extension of both doline and polje areas in the study area are parallel to the elongation of tectonic structures in the study area.
Analyzing protein concentration from intact wheat caryopsis using hyperspectral reflectance
Authors: Zhang Xiaomei , Hou Xiaoxiang , Su Yiming , Yan XiaoBin , Qiao Xingxing , Yang Wude , Feng Meichen , Kong Huihua , Zhang Zhou , Shafiq Fahad , Han Wenjie , Li Guangxin , Chen Ping , Wang Chao 
Published: May 2023

Abstract
Winter wheat grain samples from 185 sites across southern Shanxi region were processed and analyzed by using a non-destructive approach. For this purpose, spectral data and protein content data of grain and grain powder were obtained. After combining six types of pre-processed spectra and four types of multivariate statistical models, a relationship hyperspectral datasets and grain protein content is presented. It was found that the hyperspectral reflectance of winter wheat grain and powder was positively correlated with the protein contents, which provide the possibility for hyperspectral quantitative assessment. The spectral characteristic bands of protein content in winter wheat extracted based on the SPA algorithm were proved to be around 350–430 nm; 851–1154 nm; 1300–1476 nm; and 1990–2050 nm. In powder samples, SG-BPNN had the best monitoring effect, with the accuracy of Rv2 = 0.814, RMSEv = 0.024, and RPDv = 2.318. While in case of grain samples, the SG-SVM model exhibited the best monitoring effect, with the accuracy of Rv2 = 0.789, RMSEv = 0.026, and RPDv = 2.177. Based on the experimental findings, we propose that a combination of spectral pretreatment and multivariate statistical modeling is helpful for the non-destructive and rapid estimation of protein content in winter wheat.