bluuebunny/crossref_metadata_2025_split huggingface dataset:
A high affinity switch for cAMP in the HCN pacemaker channels
Authors: Porro Alessandro , Saponaro Andrea , Castelli Roberta , Introini Bianca , Alkobot Anahita Hafez , Ranjbari Golnaz , Enke Uta , Kusch Jana , Benndorf Klaus , DiFrancesco Dario , Thiel Gerhard , Moroni Anna 
Published: June 2023

Abstract
Binding of cAMP to Hyperpolarization activated cyclic nucleotide gated (HCN) channels facilitates pore opening and underlies beta adrenergic stimulation of heart rate. It is unclear why the isolated cyclic nucleotide binding domain (CNBD) displays in vitro ten to hundred times less affinity for cAMP than the full-length channel in patch experiments (micromolar vs nanomolar). Here we resolve the incongruity by showing that HCN channels are endowed with an affinity switch for cAMP, so far overlooked. Alfa helices D and E (αDE), downstream of the CNBD, form a helix-turn-helix motif that binds to and stabilizes the CNBD in a high affinity state. These helices were simply not included in previous experiments conducted with the isolated CNBD fragment of HCN2 and their addition increases the KD by about 40-fold in this fragment, while their deletion in patch clamp experiments, reduces the EC50 of HCN4 current by the same extent. The results further highlight that αDE helices interact with the same element of the CNBD, the alfa C helix (αC) that is the target of another controller of HCN cAMP affinity, the beta subunit TRIP8b. Our results uncover a mechanism whereby changes in binding affinity, rather than changes in cAMP concentration, can modulate HCN channels, adding another layer to the complex regulation of their activity.
Effect of voltage and amperage alterations in CBCT on metal artifact generation caused by exomass
Authors: Moshfeghi Mahkameh , Ahsaie Mitra Ghazizadeh , Jamalzadeh Navid 
Published: June 2023

Abstract
Objectives: This study assessed the effect of voltage and amperage alterations in cone-beam computed tomography (CBCT) on metal artifact generation in the exomass.
Materials and Methods: In this in vitro experimental study, titanium dental implants and teeth with cobalt-chromium (Co-Cr) intracanal post, and amalgam build-up restorations were placed in the exomass in a dry human mandible unilaterally and bilaterally. Ballistic gelatin was used for soft tissue simulation. The mandible was scanned by CS8100 3D-Carestream CBCT scanner with 5 x 5 cm field of view (FOV), 60 and 90 kVp voltage, and 2 and 10 mA amperage. The mean gray value (GV) in the region of interest (ROI) at the center of a polypropylene tube containing dipotassium phosphate was calculated on 10 axial sections using OnDemand software. The mean GV and its standard deviation (SD) represented the amount of generated artifacts. Data were analyzed by R software.
Results: Increasing the amperage increased the mean GV and decreased its SD (P<0.05). Increasing the voltage decreased the mean GV and its SD (P<0.05). Metal objects in the exomass significantly decreased the mean GV such that the lowest mean GV and the highest SD was recorded in presence of Co-Cr post followed by titanium implant, and amalgam build-up (P<0.05). Unilateral presence of metal objects had a significantly smaller effect on the mean GV and its SD (P<0.05).
Conclusion: Increasing the amperage and voltage decreased metal artifact generation in the exomass. Metal artifacts in the exomass can affect the GV and cause image distortion.
Effects of different feeding regimens on co-rearing of Labeo gonius and L. rohita: Unveiling growth, survival, and condition in concrete tanks
Authors: Debnath Chandan , Mahanta Prasanta 
Published: June 2023

Abstract
The study investigated the effects of different feeding treatments on the growth, survival, and condition of co-reared Labeo gonius and L. rohita in a cemented tank system (size: 7.25 m X 1.85 m X 0.85 m each). Fish were housed at a density of 10 fish/ m2, maintaining a balanced 1:1 ratio between the two species. Pellet feed (PF) at daily rates of 2%, 4%, and 6% of fish biomass, and conventional feed (CF) consisting of rice bran and mustard oil cake at a daily rate of 2% were compared. The study spanned 60 days and monitored various parameters, including growth, survival, condition factor, hepatosomatic index (HSI), viscerosomatic index (VSI), and monthly water quality assessments. CF-fed fish had a specific growth rate of 0.96% per day, while PF-fed fish showed a range from 1% to 2.9% per day. Survival rates were 84.5% for CF-fed fish and 86.5-91.5% for PF-fed fish. The feed conversion ratio was 1.6 for CF and 1.4-1.45 for PF. PF-fed fish also had higher condition (b>3), HSI ranging from 0.93% to 1.87%, and VSI ranging from 4.1% to 7.6%. L. rohita exhibited better growth, while L. gonius showed higher survival in all tanks. In conclusion, PF is a more beneficial feeding option than CF for co-rearing L. gonius and L. rohita, particularly in hilly environments. It is recommended to administer PF at a rate equivalent to 6% of fish biomass for optimal results. These findings provide valuable guidance for simultaneously rearing L. rohita and L. gonius, facilitating the adoption of appropriate feeding strategies.
Eligibility for needle aspiration of breast abscess and factors associated with adverse outcomes: A prospective cohort in two tertiary hospitals
Authors: Khamis Maulid Abdalla , Olasinde Anthoney Ayotunde , Molen Selamo Fabrice , Kyomukama Lauben , Kiswezi Ahmed , Muhumuza Joshua , Okedi Xaviour Francis 
Published: June 2023

Abstract
Introduction
Breast abscess is seen when mastitis is not treated appropriately in time resulting in accumulation of pus. Even though studies show that aspiration of breast abscess is superior to incision and drainage, most of the centers in low income countries like Uganda still practice incision and drainage as the first line surgical approach, yet it is associated with poor outcome. This study aimed at determining the proportion of breast abscesses eligible for needle aspiration and factors associated with adverse outcomes in a low income country.
Methods
This was a prospective cohort conducted at two regional referral hospitals in western Uganda in which all patients that presented with breast abscess were assessed for legibility of needle aspiration. Patients with breast abscess that underwent ultrasound guided needle aspiration were followed up for 21 days to determine the outcomes. Analysis was done using binary logistic regression in SPSS version 22 to determine the factors.
Results
During the study period, 120 females with breast abscesses were enrolled with majority aged between 18–30 years 64(53.3%). Of the 120, only 82 (68.3%) were eligible for ultrasound guided needle aspiration. The overall success rate was 89.0%, though ten patients still had pain on the 14th post-operative day (12.2%). The factors that were independently associated with adverse outcomes were: Age category 31–45 years (AOR = 0.042, P = 0. 006), being HIV positive (AOR = 7.374, P = 0.045) and having Diabetes mellitus (AOR = 24.654, P = 0.001).
Conclusion
Majority of the participants were eligible for aspiration, which method had high success rates. Health workers should consider using aspiration method for managing breast abscess among those eligible.
Approximate Solution of System of Multi-Term Fractional Mixed Volterra-Fredholm Integro-Differential Equations by BPFs
Authors: Barazandeh Yousef , Ghazanfari Bahman 
Published: June 2023

Abstract
We use the block pulse functions (BPFs) and their operational matrix to approximate solution of a system of multi-term fractional mixed Volterra-Fredholm integro-differential equations. Firstly, we homogenize it and then convert it to the system of algebraic equations. By solving the system of algebraic equations, a numerical solution is obtained.After demonstrating the method's convergence, several examples are presented. Comparing the results confirms the proposed method's applicability, accuracy, and efficiency.
Why does Ectopic Liver Tissue on the Gallbladder rarely have malignant transformation：Case Series
Authors: Zhou Yujie Zhou , Chen Jun , Liu Zhikun , Wang Kai , Wang Chao , Huan Tong , Yang Fan , Xu Xiao 
Published: June 2023

Abstract
Background
Ectopic liver tissue (ELT) is a rare developmental abnormality in which normally functioning liver tissue develops at an extra-hepatic site. Due to the atypical clinical symptoms of ELT, its clinical diagnosis is rarely easy, however, the risk of carcinogenesis is strongly increased due to its special pathological characteristics. So it should always be considered in the differential diagnosis of tumors or symptomatic mass.
Case presentation
Case 1：A 57-year-old female patient presented herself to the outpatient department of our hospital complaining of paroxysmal upper abdominal pain for one month. She was admitted due to "Multiple intrahepatic bile duct stones and chronic cholecystitis". During the surgery, an abnormally enlarged gallbladder of size about 12*8*5cmwas found. A tiny mass of about 1.2*0.6*0.3cm on the free wall of the gallbladder with a similar texture to the liver was also found. Case 2：An 87-year-old male patient presented himself to the emergency department of our hospital complaining of right upper abdomen pain for three days. He was admitted to the hospital due to "cholecystolithiasis and chronic cholecystitis". During the surgery, an abnormally enlarged gallbladder of size about 12*8*5cm. A tiny mass of about 1.2*0.6*0.3cm on the free wall of the gallbladder with a similar texture to the liver was also found. After surgery, the two patients are now in good overall condition.
Discussion and conclusions
In this two continuous cases of Ectopic liver tissue, the pathological examination and liver cancer markers “AFP” and “Glypican-3” did not support tumor lesions. Accordingly, we proposed a new hypothesis for this phenomenon, that cryptogenic functional excretory system (helps to excrete chemical carcinogens) from ELT into the gallbladder (a part of the biliary system) does exist but it cannot be found in ELT located in other organs. This phenomenon may provide a rational response to the question why does Ectopic Liver Tissue on the Gallbladder rarely have malignant transformation.
Caesarean delivery and neonatal mortality: Evidence from selected slums in and around Dhaka city, Bangladesh- A prospective cohort study
Authors: Razzaque Abdur , Chowdhury Razib , Mustafa AHM Golam , Billah Md Arif , Naima Shakera , Shafique Sohana , Sarker Bidhan Krishna , Islam Mohammad Zahirul , Kim Minjoon , Jahangir Margub Aref , Matin Ziaul , Ferdous Jannatul , Vandenent Maya , Rahman Anisur 
Published: June 2023

Abstract
Background
This study examined the survival of new-born up to neonatal period for those who delivered caesarean vs. vaginal using a prospective cohort.
Methods
A total of 6,989 births registered during 2016–2018 and were followed for neonatal survival from the selected slums of Dhaka (North & South) and Gazipur city corporations, where icddr,b maintained the Health and Demographic Surveillance System (HDSS) since 2015. Neonatal mortality was compared by maternal and new-born characteristics and mode of delivery using Z-ratios. Logistic regression model performed for neonatal mortality by mode of delivery controlling covariates and reported adjusted odd ratios with 95% confidence interval.
Results
Out of 6,989 births registered, 27.7% were caesarean and the rest were vaginal delivery; of these births, 265 neonatal deaths occurred during the follow-up. The neonatal mortality rate was 2.7 times (46 vs. 17 per 1,000 births) for vaginal than caesarean delivered. Until 3rd day of life, the risk of death was very high for both vaginal and caesarean delivered; however, on the 1st day of life, the risk of death was 3.8 times (24.8 vs. 6.3 per 1,000 births) for vaginal than caesarean delivered. In the regression analysis, the risk of neonatal mortality was higher for vaginal than caesarean delivered (aOR = 3.01; 95% CI: 1.80, 5.04), higher for adolescent than elderly adult mother (aOR = 1.64; 95% CI: 1.06, 2.55), lower for girl than boy (aOR = 0.75; 95% CI: 0.58, 0.96), lower for mother who had 5 or more years of schooling than those who had no schooling (aOR = 0.77; 95% CI: 0.57, 1.03), higher for multiple than singleton birth (aOR = 5.50; 95% CI: 2.78, 10.23), higher for very/moderate (aOR = 4.96; 95% CI: 2.56, 6.91), and late preterm birth (aOR = 1.86; 95% CI: 1.04, 2.06) than term birth. The causes of death differ for those vaginal and caesarean delivered.
Conclusion
Our study found that caesarean delivered babies had significantly lower neonatal mortality than vaginal delivered. This could be due to the differences in quality of care as caesarean delivery is performed by highly trained staff and the mother and newborn had to stay long time at the facility.
Eosinophils Predicts Coronary Artery Dilatation and Long-term Prognosis in Children with Kawasaki Disease after Intravenous Immunoglobulin Therapy
Authors: Sun Jiaying , Sun Chaonan , Cheng Xiaoli , Qi Jing , Han Lina , Zhang Qinyao , Gua Chaojun , Jiang Jinping , Su Jianyao , Wan Jiye 
Published: June 2023

Abstract
Background: Coronary artery dilation is the main cause of poor prognosis in children with Kawasaki disease. Whether eosinophils can predict coronary artery dilation and long-term prognosis in children after intravenous immunoglobulin (IVIG) treatment for Kawasaki disease (KD).
Methods: From January 2018 to December 2020, a total of 664 children with Kawasaki disease were continuously enrolled.
Results: With a sensitivity of 77.78% and a specificity of 74.38%, the optimal cut-off value for predicting coronary artery dilatation is eosinophils ≥ 0.24. According to the cut-off value, the children were separated into two groups: eosinophils<0.24 group (n = 461) and eosinophils ≥ 0.24 group (n = 203). Eosinophils ≥ 0.24 as the ideal cut-off value for predicting cardiovascular death with an area under the curve (AUC) of 0.772 [95% confidence interval (CI): 0.720 ~ 0.823, P < 0.001], a sensitivity of 72.88%, and a specificity of 78.57%. The incidence of cardiovascular death, heart failure and multiple organ dysfunction was greater in the eosinophils ≥ 0.24 group, although there was no significant difference in the incidence of pericarditis procedures following IVIG between the two groups. Eosinophils ≥ 0.24 was also an independent predictor of cardiovascular death (hazard ratio = 4.95, 95% confidence interval (CI):2.98 ~ 8.23, P < 0.001). However, eosinophils have a lower sensitivity (23.08%) for predicting the recurrence of Kawasaki disease.
Conclusion: eosinophils ≥ 0.24 was independently associated with coronary artery dilatation and poor clinical outcomes for children in Kawasaki disease after IVIG treatment.
Prediction of contact characteristics of abrasive belt compliant grinding for aircraft blades
Authors: Duan Jihao , Wu Zhuofan , An Jiale , Wang Dou , Gao Feng , Huai Wenbo 
Published: June 2023

Abstract
Due to the characteristics of thin-walled curved surface, wall thickness variations and processing cantilever fixtures, the mechanical state of the different contact positions of aircraft engine blades varies significantly during the grinding process. The different contact interactions between contact wheel and blade result in changes of material removal efficiency and surface quality. To achieve contact state control during blade grinding process, a novel flexible abrasive belt grinding device was designed and developed considering the compliance of rubber contact wheel. The significant effect of compliance parameters on grinding contact state was verified through simulation. The grinding contact pressure distribution and normal contact force at different positions in the blade width and length directions were studied, and a prediction model for the maximum contact pressure and normal contact force was established based on BP neural networks. The results showed that with the increase in contact wheel compliance, the effective contact range increased, the pressure distribution gradually became uniform, and showed a double-elliptical distribution. The maximum contact pressure was significantly reduced, with a reduction of up to 46.00%. As the grinding contact position moved towards the weak rigidity area of the blade, the contact pressure distribution became more uniform. And the normal contact force was significantly reduced, with a maximum reduction of 68.49%. The mean average percentage error (MAPE) of the prediction model was small, verifying the effectiveness of the model. The research results of this manuscript laid a foundation for achieving consistent control of blade grinding material removal rate through contact wheel compliance adjustment.
USP22 promotes tumorigenesis and progression by a FKBP12/mTORC1/autophagy positive feedback loop in hepatocellular carcinoma
Authors: Ye Qianwei , Zhou Wei , Xu Shengjun , Que Qingyang , Zhan Qifan , Zhang Lincheng , Zheng Shusen , Ling Sunbin , Xu Xiao 
Published: June 2023

Abstract
Background: Ubiquitin-specific protease 22 (USP22) was a potential cancer stem cell (CSC) marker and could promote hepatocellular carcinoma (HCC) stemness upon tumor protein P53 (TP53) inactivation, which have been viewed as drivers of tumorigenesis. Here, we determined the role of USP22 in hepatotumorigenesis, illustrated the underlying mechanism as well as explored the therapeutic significance of USP22 in HCC.
Methods: A mouse model of orthotopic HCC was established to investigate the role of USP22 during tumorigenesis. RNA-sequencing was performed to find downstream genes. Cell proliferation, counting, migration and colony formation were used to detect USP22 function in HCC cells. Immunoprecipitation and ubiquitination assay were applied to elucidate the mechanism of USP22 and FK506-binding protein 12 (FKBP12). HCC patients’ samples after liver transplantation (LT) were used to demonstrate the clinical significance.
Results: Usp22 accelerated c-Myc/NRasGV12 induced HCC in mice and mammalian target of rapamycin complex 1 (mTORC1) pathway was activated in the downstream. Besides, USP22 overexpression resulted in increased tumorigenic properties, which could be reversed by rapamycin in vitro and in vivo. USP22 activated mTORC1 by direct binding to FKBP12 and deubiquitinating it. In addition, activated mTORC1 further stabilized USP22 via inhibiting autophagic degradation in turn. Clinically, LT recipients with high USP22 expression tended to benefit from the use of sirolimus.
Conclusions: USP22 promotes tumorigenesis and progression by a FKBP12/mTORC1/autophagy positive feedback loop in HCC. USP22 could be an effective biomarker for selecting eligible recipients with HCC to receive anti-mTOR-based therapies after LT.
A Case Study on Estimation of Sensor Data Generation in Smart Cities and the role of Opportunistic Networks in Sensor Data Collection
Authors: Gandhi Jay , Narmawala Zunnun 
Published: June 2023

Abstract
The across-the-global diffusion of smartphones is initiating an enormous growth of data traffic. Soon, the existing cellular networks will suffer from traffic overload issues, and establishing additional infrastructure might be the costlier solution. An offloading of the traffic to different networks is considered a promising approach. Opportunistic offloading refers to diverting traffic from a cellular network to Opportunistic Networks (OppNets) by utilizing nearby devices having idle computing resources through opportunistic contacts. As a case study, we analyzed the number of sensors needed and the amount of data generated in Ahmedabad and Gandhinagar by considering the smart city scenario. The paper comprehensively reviews OppNets in data offloading for various scenarios and applications of smart cities. We further point out the important future challenges, problems, and applications.
Response to high dose ipilimumab plus temozolomide after progression on standard or low dose ipilimumab in advanced melanoma: a retrospective analysis
Authors: Williamson Julie , Fadlullah Muhammad Zaki Hidayatullah , Kovacsovics-Bankowski Magdalena , Gibson Berit , Swami Umang , Erickson-Wayman Alyssa , Jamison Debra , Sageser Dan , Jeter Joanne , Bowles Tawnya , Cannon Donald M. , Haaland Ben , Schroeder Joyce D , Nix David , Atkinson Aaron , Hyngstrom John , McPherson Jordan , Tan Aik-Choon , Hu-Lieskovan Siwen 
Published: June 2023

Abstract
Background: Despite advancements in checkpoint inhibitor-based immunotherapy, patients with advanced melanoma who have progressed on standard dose ipilimumab (Ipi) + nivolumab continue to have poor prognosis. Several studies support a dose-response activity of Ipi, and one promising combination is Ipi 10mg/kg (Ipi10) + temozolomide (TMZ).
Methods: We performed a retrospective cohort analysis of patients with advanced melanoma treated with Ipi10+TMZ in the immunotherapy refractory/resistant setting (n = 6), using similar patients treated with Ipi3+TMZ (n = 6) as comparison. Molecular profiling by whole exome sequencing (WES) and RNA-seq of tumors harvested through one responder’s treatment was performed.
Results: With a median follow up of 119 days, patients treated with Ipi10+TMZ had statistically significant longer median progression free survival of 144.5 days (range 27–219) vs 44 (26–75) in Ipi3+TMZ, p=0.04, and a trend for longer median overall survival of 154.5 days (27–537) vs 89.5 (26–548). All patients in the Ipi10 cohort had progressed on prior Ipi+Nivo. WES revealed only 12 shared somatic mutations including BRAF V600E. RNA-seq showed enrichment of inflammatory signatures, including interferon responses in metastatic lesions after standard dose Ipi + nivo and Ipi10 + TMZ compared to the primary tumor, and downregulated negative immune regulators including Wnt and TGFb signaling.
Conclusion: Ipi10+TMZ demonstrated efficacy including dramatic responses in patients with advanced melanoma refractory to prior Ipi + anti-PD1, even with CNS metastases. Molecular data suggest a potential threshold of Ipi dose for activation of sufficient anti-tumor immune response, and higher dose Ipi is required for some patients.
Ultrasound-Based Intestinal Cleansing Protocol for Critically Ill Adult Patients
Authors: NI xiumei , CAI Yuehong , XU Fengling , LI Taozi , XU Gongxia , WANG Jihai , YE Mao , HU Shaohua 
Published: June 2023

Abstract
Aims and Objectives:To investigate the application of ultrasound in intestinal cleansing for critically ill adult patients.
Patients: A total of 49 critically ill patients admitted to the intensive care unit (ICU) from December 2020 to April 2022 were selected and then randomly divided into a control group and an intervention group, with 25 cases in the control group and 24 cases in the intervention group.
Design and Methods:The control group was offered routine intestinal cleansing measures, while the intervention group was given targeted laxatives, catharsis, or motility-promoting drugs according to the results of a gastrointestinal ultrasound evaluation. Before intervention and 24 hours after intervention, we compared several features between the two groups, including the transverse diameters of the ascending colon and the descending colon, time of first defecation, defecation frequency, number of defecations, and the abdominal circumferences of the ascending colon and the descending colon.
Results: After intervention, the transverse diameters ofthe ascending colon and the descending colon in the intervention group were 3.21±0.39 cm and 3.09±0.26 cm, respectively, which were lower than those in the control group (P < 0.05). Additionally, in the intervention group, the first defecation time and abdominal circumference were lower than in the control group (P < 0.05), while the total number of defecations and the number of defecations within 24 hours were higher than in the control group (P < 0.05). There was a significant difference between the two groups regarding abdominal circumference.
Conclusions: The ultrasound-based ICU enteric cleansing program achieves satisfactory intestinal cleansing and relieves the pain of patients with multiple enemas through accurate evaluation and targeted cleansing, which makes it worthy of promotion and application.