bluuebunny/crossref_metadata_2025_split huggingface dataset:
How to Develop Patient Centred Consulting during Workplace Learning in Postgraduate Medical Education? Opening the Black Box Using the Framework of Four Narrative Profiles for Consultation Performance
Authors: Timmerman Angelique , Pawlikowska Teresa , van der Vleuten Cees , Muris Jean 
Published: January 2025

AbstractContextFour narrative profiles were previously developed as an evidence-informed framework for reflection and feedback on consultation performance in medical education. The profiles are grounded in four typologies mapped onto a conceptual framework, using the dimensions of doctor patient interaction (DPI) and medical expertise (ME) to classify overall performance.ObjectiveContent validation of the narrative profiles derived from routine clinical consultations to inform a developmental roadmap for patient-centred consulting.MethodsA qualitative study was performed in Family Medicine (FM) residency training, in which 11 first year and 7 third year FM trainees participated. The same FM assessor (n=11) observed a series of encounters of the trainee every three months during their training year, after which recurrent behaviours were described in a feedback report and overall consultation performance classified in one of the four typologies. Feedback reports (n=56) were categorised for each typology, coded on recurrent behaviours and then compared with the concordant narrative profile content.ResultsWe identified overlapping recurrent behaviours and communication themes between the narrative profiles and feedback reports. For typology 1 (DPI+, ME-), the communication approach shows natural alignment with the patient, more exploration of patient concerns is needed, and the treatment plan needs connection to the reasons for consulting. In typology 2, active listening provides room for patient experience, yet patient centredness risks losing focus, while exploring of reasons for consulting is present. Inadequate responses to patient cues and losing structure in the consultation for typology 3, result in not grounding reassurance in clinical findings. For typology 4, curiosity towards patient’s concerns, may support understanding of symptoms and expectations.ConclusionA developmental road map for patient centred consulting was outlined, covering balancing medical tasks with exploring patient cues, enacting leadership in agenda management and integrating medical expertise in applied communication, providing focus and language for individual learning trajectories.
Is the human face a biomarker of health? – a scoping review
Authors: Obrochta Weronika M. , Bartecka Paula , Klaś Katarzyna , Klimek Magdalena , Marcinkowska Urszula M. 
Published: January 2025

AbstractThere is a widespread opinion that facial features could provide an important cue to individual’s health and are a biomarker of a human developmental stability. Taking evolutionary lens, they are interpreted to be a signal physical and cognitive health. However, research to date does not clearly support this assumption. This is the first review that explores the association between various aspects of health and facial features, namely symmetry, averageness or sexual dimorphism in adults. We searched electronic databases including Web of Science, MEDLINE PubMed, Scopus and Embase. We followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines for reporting of our results. Of the 702 screened articles, 17 were eligible for inclusion. Studies presented a varied outcomes between facial features and cardiovascular health; immunocompetence; oxidative stress level; cortisol level; reproductive health, cognitive health and general physical health. This review presents mixed and inconclusive answer for the question whether facial features can serve as indicators of health. The results deepen our knowledge of the relationship between facial features and health outcomes and warrant caution when interpreting face as a biomarker of health. Protocol: Open Science Framework,https://osf.io/dv9pu/.
Warning system for Extreme weather events, Awareness Technology for Healthcare, Equitable delivery, and Resilience (WEATHER) Project: A mixed methods research study protocol
Authors: Lynch Mary , Harris Fiona , Ierna Michelle , Mahomed Ozayr , Henriquez-Mui Fiona , Gebreslasie Michael , Ndzi David , Viriri Serestina , Shakir Muhammad Zeeshan , Dickinson Natalie , Miller Caroline , Nadesan-Reddy Nisha , Nkwanyana Fikile , Spencer Llinos Haf , Naidoo Saloshni 
Published: January 2025

AbstractBackgroundExtreme weather events (EWEs) in KwaZulu-Natal province, South Africa are increasingly common causing disease outbreaks, loss of homes, damage to infrastructure, increased mortality and life as well as increasing the pressure on the health system. An Early Warning System (EWS) intervention is needed to alert communities and government increasing preparedness for floods, improving resilience of health services to predict, detect and respond to emergencies and disease outbreaks. The aim of this mixed methods study is to develop, implement and evaluate an EWS as an alert system for communities and government agencies to detect, identify and prevent disease outbreaks strengthening the health system response.MethodsThe study sites are eThekwini and Ugu, municipalities in KwaZulu-Natal province, South Africa and will comprise the selected community and health facilities in selected sites in these two health districts. This transdisciplinary project will build on prior engagement with communities/health providers (e.g., communities, professionals, policy makers) integrating a participatory, co-creation approach. To build the foundation for this study and understand the impact of climate change on disease outbreaks, a systematic review will be conducted. We will also design, develop and evaluate the performance of an EWS and will link data on the two (rural and urban) healthcare and environmental contexts and integrate EWS for change, clarify how these will be delivered for vulnerable communities, and test and adapt the EWS. Following, an assessment of the disease burden at primary health care clinics in Ugu and eThekwini will be undertaken.Subsequently, an examination of the health needs along with community experiences, during, an EWE, as well as assessment of the health systems resilience in responding to an EWE. Finally, an evaluation will be conducted on the design, development and evaluation of the performance of an EWS intervention and an evaluation and costing of the implementation of the EWS. Ethics for the project will be sought from the relevant ethical committees and informed consent taken by voluntary participants in the study.DiscussionBased on the study findings, recommendations will be made to the Department of Health with respect to an early warning system and health systems resilience.Trial registrationNot applicableFundingThis study is funded by funded by a Research and Innovation for Global Health Transformation National Institute for Health and Care Research (NIHR) award (NIHR204825).
Affiliative behaviours regulate allostasis development and shape biobehavioural trajectories in horse
Authors: Valenchon Mathilde , Reigner Fabrice , Lefort Gaëlle , Adriaensen Hans , Gesbert Amandine , Barrière Philippe , Gaude Yvan , Elleboudt Frederic , Lévy Isabelle , Ducluzeau Camille , Dupont Joëlle , Lainé Anne-Lyse , Uszynski Ivy , Dardente Hugues , Poupon Cyril , Lansade Léa , Calandreau Ludovic , Keller Matthieu , Barrière David André 
Published: January 2025

AbstractSocial interactions shape both physiological and behavioural development of offspring and poor care/early caregiver loss are known to promote negative outcomes in adulthood in both animals and humans. How affiliative behaviours impact future development of offspring remains unknown. Here, we usedEquus caballus(domestic horse) as a model to investigate this question. By coupling magnetic resonance imaging, longitudinal biobehavioural assessment and advanced multivariate statistical modelling we found that maternal presence during childhood promotes maturation of brain territories involved in both social behaviour (anterior cingulate, retrosplenial cortex) and physiological regulation (hypothalamus, amygdala). Additionally, we found that offsprings benefiting from prolonged maternal presence showed higher default mode network (DMN) connectivity, improved social competences, more efficient feeding behaviours, and metabolic profiles. The present study underscores the salient role of social interactions for the development of allostatic regulation in offspring.
GCN5a is a telomeric lysine acetyltransferase whose loss primesToxoplasma gondiifor latency
Authors: Dey Vishakha , Holmes Michael J. , Srivastava Sandeep , Wilson Emma H. , Sullivan William J. 
Published: January 2025

ABSTRACTToxoplasma gondiiis a protozoan parasite that causes persistent infection in warm-blooded vertebrates by undergoing differentiation from a replicative stage (tachyzoites) to a latent encysted stage (bradyzoites). Stage differentiation is critical for transmission and pathogenesis and relies on gene regulation driven by a network of transcription and epigenetic factors. We previously found in non-cystogenic type I RH strain parasites that the lysine acetyltransferase (KAT), GCN5a, is dispensable in tachyzoites but required to upregulate stress-response genes, suggesting a link with bradyzoite conversion. To address this possibility, we generated endogenously tagged GCN5a parasites and a genetic knockout in cystogenic type II Pru strain. We show that GCN5a protein, but not mRNA, increases during differentiation and complexes with unique protein partners, most of which contain AP2 domains. Pru strain tachyzoites lacking GCN5a augment bradyzoite-specific gene expression in the absence of stress. Loss ofGCN5aslowed tachyzoite replication and heightened sensitivity to bradyzoite conversion but resulted in smaller cyst sizes compared to wildtype. Using CUT&Tag, we delineated the chromosomal occupancy of GCN5a relative to the essential KAT, GCN5b. While GCN5b localizes to coding regions, GCN5a surprisingly localizes exclusively to telomeres. These findings suggest that the loss of GCN5a leads to telomere dysfunction, which slows replication and promotes the transition to latency.IMPORTANCEToxoplasma gondiiis a single-celled parasite that persists in warm-blooded hosts, including humans, because it converts into latent tissue cysts. Switching from its replicating form into dormant cysts is a tightly regulated process that involves epigenetic factors such as lysine acetyltransferases GCN5a and GCN5b. This study is the first to examine the role of GCN5a in a cyst-formingToxoplasmastrain. We found that GCN5a protein, but not mRNA, increases during cyst development. Additionally, parasites lacking GCN5a replicate more slowly and are quicker to form cysts when stressed. We show that GCN5a and GCN5b work in different multi-protein complexes and localize to different areas of the genome; while GCN5b targets promoters of gene coding regions, GCN5a is exclusively found at telomeric regions. Our findings suggest a novel role for GCN5a in telomere biology that, when depleted, produces a fitness defect that favors development of latent stages.
Microbiota assembly of specific pathogen-free neonatal mice
Authors: Kennedy Elizabeth A. , Weagley James S. , Kim Andrew H. , Antia Avan , DeVeaux Anna L. , Baldridge Megan T. 
Published: January 2025

AbstractBackgroundNeonatal mice are frequently used to model diseases that affect human infants. Microbial community composition has been shown to impact disease progression in these models. Despite this, the maturation of the early-life murine microbiome has not been well-characterized. We address this gap by characterizing the assembly of the bacterial microbiota of C57BL/6 and BALB/c litters from birth to adulthood across multiple independent litters.ResultsThe fecal microbiome of young pups is simple, dominated by only a few pioneering bacterial taxa. These taxa are present at low levels in the microbiota of multiple maternal body sites, precluding a clear identification of maternal source. The pup microbiota begins diversifying after fourteen days, coinciding with the beginning of coprophagy and the consumption of solid foods. Pup stool bacterial community composition and diversity are not significantly different from dams from day 21 onwards. Short-read shotgun sequencing-based metagenomic profiling of young pups enabled the assembly of metagenome-assembled genomes for strain-level analysis of these pioneerLigilactobacillus, Streptococcus,andProteusspecies.ConclusionsAssembly of the murine microbiome occurs over the first weeks of postnatal life and is largely complete by day 21. This detailed view of bacterial community development across multiple commonly employed mouse strains informs experimental design, allowing researchers to better target interventions before, during, or after the maturation of the bacterial microbiota. The source of pioneer bacterial strains appears heterogeneous, as the most abundant taxa identified in young pup stool were found at low levels across multiple maternal body sites, suggesting diverse routes for seeding of the murine microbiome.
An MRI-informed histo-molecular analysis implicates ependymal cells in the pathogenesis of periventricular pathology in multiple sclerosis
Authors: Groh Adam M.R. , Afanasiev Elia , Thevakumaran Risavarshni , Callahan-Martin Liam , Creeggan Finn , Yaqubi Moein , Zandee Stephanie , Prat Alexandre , Rudko David A. , Stratton Jo Anne 
Published: January 2025

AbstractIt is now widely recognized that the cerebrospinal fluid (CSF)-adjacent brain surfaces – namely the subpial cortical region and the ependyma-adjacent periventricular region – are uniquely susceptible to a distinct, diffuse form of pathology in multiple sclerosis. So-calledsurface-ingradients of pathology predict future disease relapses independent of classical white matter lesions and are thought to occur as a result of cytotoxic factors in the CSF. Given the underlying mechanisms drivingsurface-ingradients appear to be distinct, they represent a novel treatment target. However, exactly how cytotoxic factor entry into the brain is regulated at these CSF-facing borders is not understood, particularly at the ventricular interface. Indeed, although studies have indicated that ependymal cells may be damaged in MS, there has yet to be a comprehensive assessment of cell health in the disease. We employed ultra-high-field MRI-guided immunohistochemistry, electron microscopy, and multiomic single nucleus RNA/ATAC sequencing to deeply phenotype human ependymal cells in MS. Our data revealed that ependymal cell pathology is a direct correlate of periventricularsurface-ingradients of pathology in MS, and that the immune-responsive, reactive state assumed by ependymal cells is associated with widespread transporter and junctional protein gene dysregulation. We then further defined the gene regulatory networks underpinning the MS ependymal state, predicted ligands known to be enriched in MS CSF that could drive the emergence of this state, and tested one candidatein vivo. We found that IFNγ increased murine ependymal permeability and that conditional knockout of ependymal interferon gamma receptor 1 (Ifngr1) was sufficient to reverse this effect. Our data directly implicate ependymal cell dysregulation in the emergence of periventricular pathology in MS. More widely, we denote the modulatory capacity of CSF ligands on ependymal cell function and how this may influence the inflammatory status of the periventricular region.
The Arabidopsis dehydrin ERD14 chaperones the brassinosteroid receptor kinase BRL3 to the plasma membrane to confer drought resistance
Authors: Marquès-Bueno M.M. , Herrero-García I. , Pistillo S. , Gupta A. , Lozano-Elena F. , Blasco-Escámez D , Karampelias M. , Fàbregas N. , Strotmann V.I. , Stahl Y. , Caño-Delgado A.I 
Published: January 2025

AbstractBrassinosteroid plant hormones are involved in growth, biotic and abiotic-stress signaling, but functional analysis of its multiple receptors has largely focused on the ubiquitously expressed BRASSINOSTEROID INSENSITIVE 1 (BR1). The vascular-expressed brassinosteroid receptor kinase BRI1-LIKE 3 (BRL3) has a specific and essential role in Arabidopsis adaptation to drought and elevated temperature. However, the mechanistic understanding of BRL3 function and regulation is scant. Here, we show the dehydrin ERD14 colocalizes with BRI1 and BRL3 at the plasma membrane and physically interacts with these receptors through the ERD14Lysdomain for proper receptor recruitment.erd14mutants internalize BRL3–GFP and revert drought-resistantBRL3over-expression phenotypes. BRL3 activation and downstream signaling events, including dephosphorylation of BES1 transcription factor, depend on the chaperone function of ERD14. Our study shows how proper chaperoning by ERD14 regulates BRL3 subcellular localization and activity of the vascular receptor, prompting re-examination of models of brassinosteroid-receptor functional diversification in plant adaptation to abiotic stress from specific cells of the vascular systems.
The mediating effect of employer branding on the relationship between transformational leadership and organisational commitment in the South African telecoms sector
Authors: Makumbe William 
Published: January 2025

PurposeThis study sought to investigate the mediation effect of employer branding on the relationship between transformational leadership and organisational commitment.Design/methodology/approachBased on an extensive literature review, a structured questionnaire was designed, and data were collected from 400 managers in the South African telecoms sector. Statistical analyses were done using SMART PLS 4.FindingsThe study established that while transformational leadership positively impacted organisational commitment, employer branding partially mediated the relationship between the two variables.Originality/valueLittle is empirically known about the mediation mechanism of employer branding on the relationship between transformational leadership and organisational commitment in the South African telecoms sector. This study, therefore, brings new insights that are important for decision-making.
Feedback from the younger generation on health information shared by their parents on WeChat: types, antecedents and consequences
Authors: Li Xinyue , Liu Mandie , Song Xiaokang , Zhu Qinghua 
Published: January 2025

PurposeIn China, it is prevalent for parents to share health information on WeChat and receive feedback from their children. This study aims to investigate the feedback from younger generations regarding their parents’ health information sharing. It will examine the different types of feedback, explore the factors influencing it and analyze the outcomes that result from this feedback exchange.Design/methodology/approachThe empirical findings draw on the qualitative analysis using grounded theory. This study collects data from 34 participants (17 pairs of one young person and one parent) through in-depth interviews and WeChat chat records. Then, a theoretical model was developed through open, axial and selective coding.FindingsFeedback can be classified into five types: support, correction, perfunctoriness, ostracism and rejection as well as into “Affective-Behavioral-Cognitive” dimensions. Younger generations’ feedback types are influenced by a variety of factors, including information, emotion and individual and family-related factors. Each feedback type has distinct effects, such as altering older generations’ emotional and communication responses.Originality/valueThis pioneering study explores how younger generations in China perceive their parents’ health information sharing on social media. It highlights the importance of feedback in this context, providing actionable insights to enhance digital literacy among older adults, strengthen family bonds and enhance the spread of valuable and scientific health information online.
Moral intelligence among Muslim employees in hospitality settings: scale development and validation
Authors: Salem Mennaalla Hassan , Selem Kareem M. , Ahmed Huda Shaaban Hassan , Mohamed Hanan Ahmed 
Published: January 2025

PurposeAs upscale hotels have guests from diverse backgrounds and cultures, their Muslim employees should respect each. Consequently, this paper seeks to develop a multi-dimensional scale that measures Muslim employees’ moral intelligence within the hospitality domain.Design/methodology/approachThe following dimensions: fear of Allah, humility, generosity and tolerance were included in this new scale. To develop this scale, this paper incorporates a multistage approach.FindingsEmploying ADANCO-PLS v.2.4, the nomological validity of this new scale was examined. These dimensions will help Muslim employees in hospitality contexts enhance their moral intelligence, which can result in their skill development, increased job delight and retention since they will feel appreciated and encouraged toward their ethical growth.Practical implicationsUpscale hotels can benefit by creating workplace cultures of giving for guest experiences. When Muslim employees are ready to take it up a notch, they contribute exceptional guest experiences and deeper relationships. This leads to higher levels of customer loyalty rates with revisit intentions.Originality/valueGiven moral judgments are essential in hospitality businesses; this paper highlighted using moral intelligence attitudes among Muslim employees.
Design thinking in action: a quantitative study of design thinking practices in innovation projects
Authors: Dell’Era Claudio , Magistretti Stefano , Candi Marina , Bianchi Mattia , Calabretta Giulia , Stigliani Ileana , Verganti Roberto 
Published: January 2025

Purpose
Design thinking is widely recognized as an effective problem-solving approach in the professional and academic world, albeit with varying interpretations. It has been studied in multiple forms – as a tool, a practice, a skill and a mindset – leading to ongoing debates about its fundamental nature. This study aims to explore the use of design thinking in practice and determine how its application varies depending on the characteristics of the innovation projects, namely, the types of goals pursued and the level of uncertainty involved.

Design/methodology/approach
Using a survey methodology and a knowledge-intensive empirical setting, this study analyzes a data set of 221 innovation consulting projects based on design thinking conducted by European consulting firms and design agencies.

Findings
By analyzing the survey data, the authors identify six distinct sets of design thinking practices: discovering user needs, understanding the problem addressed, challenging existing assumptions, navigating the problem-solution pair, ideating through visualizations and learning through prototypes. The authors also identify configurations of these design thinking practices that are used to address different innovation project goals and levels of uncertainty.

Practical implications
The study draws attention to the need for design thinking practitioners to be aware of how different innovation project goals and levels of uncertainty can be pursued/addressed through the use of alternative configurations of design thinking practices.

Originality/value
To the best of the authors knowledge, this study is one of the first large-scale quantitative analyses of the nature of design thinking in action, providing a solid foundation for future research on design thinking.