bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function
Authors: Johnson Christopher J. , Kulkarni Akhil , Buxton William J. , Hui Tsz Y. , Kayastha Anusha , Khoja Alwin A. , Leandre Joviane , Mehta Vanshika V. , Ostrowski Logan , Pareizs Erica G. , Scotto Rebecca L. , Vargas Vanesa , Vellingiri Raveena M. , Verzino Giulia , Vohra Rhea , Wakade Saurabh C. , Winkeljohn Veronica M. , Winkeljohn Victoria M. , Rotterman Travis M. , Stolfi Alberto 
Published: May 2023

AbstractTunicates are marine, non-vertebrate chordates that comprise the sister group to the vertebrates. Most tunicates have a biphasic lifecycle that alternates between a swimming larva and a sessile adult. Recent advances have shed light on the neural basis for the tunicate larva’s ability to sense a proper substrate for settlement and initiate metamorphosis. Work in the highly tractable laboratory model tunicateCiona robustasuggests that sensory neurons embedded in the anterior papillae of transduce mechanosensory stimuli to trigger larval tail retraction and initiate the process of metamorphosis. Here, we take advantage of the low-cost and simplicity ofCionaby using tissue-specific CRISPR/Cas9-mediated mutagenesis to screen for genes potentially involved in mechanosensation and metamorphosis, in the context of an undergraduate “capstone” research course. This small screen revealed at least one gene,Vamp1/2/3, that appears crucial for the ability of the papillae to trigger metamorphosis. We also provide step-by-step protocols and tutorials associated with this course, in the hope that it might be replicated in similar CRISPR-based laboratory courses whereverCionaare available.
Revealing and reshaping attractor dynamics in large networks of cortical neurons
Authors: Beer Chen , Barak Omri 
Published: May 2023

AbstractAttractors play a key role in a wide range of processes including learning and memory. Due to recent innovations in recording methods, there is increasing evidence for the existence of attractor dynamics in the brain. Yet, our understanding of how these attractors emerge or disappear in a biological system is lacking.By following the spontaneous network bursts of cultured cortical networks, we are able to define a vocabulary of spatiotemporal patterns and show that they function as discrete attractors in the network dynamics. We show that electrically stimulating specific attractors eliminates them from the spontaneous vocabulary, while they are still robustly evoked by the electrical stimulation. This seemingly paradoxical finding can be explained by a Hebbian-like strengthening of specific pathways into the attractors, at the expense of weakening non-evoked pathways into the same attractors. We verify this hypothesis and provide a mechanistic explanation for the underlying changes supporting this effect.Author summaryThere are many hints that could evoke the same memory. There are many chains of evidence that could lead to the same decision. The mathematical object describing such dynamics is called an attractor, and is believed to be the neural basis for many cognitive phenomena. In this study, we aimed to deepen our understanding of the existence and plasticity of attractors in the dynamics of a biological neural network. We explored the spontaneous activity of cultured neural networks and identified a set of patterns that function as discrete attractors in the network dynamics. To understand how these attractors evolve, we stimulated the network to repeatedly visit some of them. Surprisingly, we observed that the stimulated patterns became less common in the spontaneous activity, while still being reliably evoked by the stimulation. This paradoxical finding was explained by the strengthening of specific pathways leading to these attractors, alongside the weakening of other pathways. These findings provide valuable insights into the mechanisms underlying attractor plasticity in biological neural networks.
Influence of laser scanning power on microstructure and tribological behavior of NI-composite claddings fabricated on TC4 titanium alloy
Authors: Miah Md. Helal , Singh Chand Dharmahinder , Malhi Gurmail Singh , Khan Shahrukh 
Published: May 2023

Purpose
The demand for titanium alloys has received massive attention in the aerospace and automotive industry owing to their magnificent electrochemically compatibility and corrosion resistance, high strength at elevated temperatures and high strength-to-weight ratio. Although titanium alloy has impressive mechanical properties, they are challenging to machine or metal form due to its poor heat conductivity, high chemical reactivity, low modulus of elasticity, high friction coefficient and difficult lubricant that limits its application field and increases wear. However, surface treatment coating with the strong metallurgical bond between the titanium alloy matrixes is novel technique to resolve these challenges. This research will illustrate the influence of laser scanning power on the microstructure and tribological behavior of Nickel (NI)-composite claddings fabricated on TC4 titanium alloy to realize the strong metallurgical bond between the titanium alloy and NI-composite coating.

Design/methodology/approach
In this research, TiC/TC4 alloy nanocomposites were fabricated based on different laser power and temperatures. TC4 has been selected as a base material instead of TiC for the strong metallurgical bond between the titanium alloy matrixes. Then Ni-composite coating was used as the surface treatment coating on TC4 by laser cladding (LC) technique. The Ni-based alloy coating material powder is good self-fluxing, has high-temperature resistance and is analytically pure with 200 mesh, which can easily overcome the various challenges of titanium alloy. The chemical properties of Ni composite coating include 31.2% Chromium, 8%Titenium and 3.6% Carbon. The prepared surface treatment coating characterization and microstructure behavior are analyzed using optical micrograph, X-ray diffraction, scanning electron microscopes, energy dispersive spectroscopy and electron probe micro analyzer methods.

Findings
It is evident that at the beginning of the experiment, if the laser power increased, the quality of the coating increased. An optimal quality of the coating is found when the laser scanning power about 12.55 kJ/cm2. Further increased laser power diminished the quality of the coating because the material plasticity had deteriorated. The TiC ceramic particle reinforced phase is dispersed into a two-phase solid solution of β-Ti and γ-Ni. The micro-hardness of the used coating is greater than the base alloy.

Originality/value
This research has practical value in the modern aerospace and automobile industry to increase the application of titanium alloy.
Cybergogy paradigms for technology-infused learning in higher education 4.0: a critical analysis from global perspective
Authors: Asad Muhammad Mujtaba , Malik Aisha 
Published: May 2023

PurposeUnprecedented progressions in the sector of Information and Communication Technologies (ICTs) have led to the trendsetting of Education 4.0 paradigms. A byproduct of breakthroughs in Education 4.0 is the “Cybergogy” paradigm that particularly stimulates learning and builds competencies in a virtual environment for the new era. Toward that, the objective of this review paper is to explore how cyber-physical learning is being adopted within cybergogy paradigms with its current state-of-the-art, traits, design models, applications and difficulties in Higher Education Institutions (HEIs) around the globe.Design/methodology/approachA narrative review of literature has been conducted in which major electronic bibliographic databases are targeted for study selection. Seven themes have emerged from the literature search that descript the resourcefulness, inventiveness and challenges of cyber-technology in transferring learning and interaction in and outside HEI boundary walls.FindingsGrounded on the findings, it is valuable to mention that cybergogy media facilitates an array of options for collaboration, information gathering, intellectual discussions, the convenience of access, etc. among higher education learners. Consisting of interconnected technology, distinctive intricate digital systems and physical elements working together, the trend has implementation efforts of a smart learning institution for providing engaged learning and advanced skills training. However, there are certain implementation risks and consequences that must be considered thoughtfully.Practical implicationsThis review can be used as an effective guide in designing cyber-technology-mediated teaching instruction and strategies for supporting collaborations and inventive learning in higher academia. Future researchers interested in exploring cyber-physical blends and media usage in education will also be facilitated with this review.Originality/valueThis literature review is unique because it details significant practices of collaborative learning for the twenty-first century through cybergogy in Higher Education 4.0 that was not examined previously.
The impact of nutrition labeling on consumer perceptions of wine
Authors: Popovich Deidre , Velikova Natalia 
Published: May 2023

Purpose
The purpose of this study is to examine how consumers perceive nutrition labeling on wine and how this information impacts healthiness perceptions of wine.

Design/methodology/approach
A series of four experiments focused on healthiness perceptions and purchase likelihood.

Findings
Consumers who read wine labels rate wine as significantly less healthy. Sugar content affects healthiness perceptions of wine more than calories. Changing the serving size on the label moderates these effects. Consumers high in dietary restraint process this nutrition information differently.

Research limitations/implications
Future research could examine actual purchase behavior using retail data.

Practical implications
This study has implications for consumers, manufacturers and public policy. While currently most consumers are not motivated to read a nutrition label on wine, specific nutrition information can impact consumer perceptions of wine. Consumer education is recommended.

Originality/value
Research on nutrition labeling of alcohol specifically has been very limited.
Emotional capital in schools: principals' caring leadership as a moderator of the mediated association between emotional geographies and school climate
Authors: Berkovich Izhak 
Published: May 2023

PurposeThe caring leadership of principals is viewed as a professional method for utilizing emotional capital. The present study investigated the moderating relationship of caring leadership with the associations between emotional geographies, emotional distance and school climate.Design/methodology/approachData from a cross-sectional survey of 305 Israeli teachers working in public schools were used to investigate a model of moderated mediation.FindingsCaring leadership of principals moderates the association of emotional distance with school climate and is related to mediated associations between emotional geographies and school climate.Originality/valueThis is the first study to combine Hargreaves' theory of emotional geographies of schools with principals' caring leadership, providing insight on the role of school leadership in bridging fundamental gaps between key actors and treating crippling emotional dynamics.
Queueing networks for supporting container storage and retrieval
Authors: Legato Pasquale , Mazza Rina Mary 
Published: May 2023

PurposeAn integrated queueing network focused on container storage/retrieval operations occurring on the yard of a transshipment hub is proposed. The purpose of the network is to support decisions related to the organization of the yard area, while also accounting for operations policies and times on the quay.Design/methodology/approachA discrete-event simulation model is used to reproduce container handling on both the quay and yard areas, along with the transfer operations between the two. The resulting times, properly estimated by the simulation output, are fed to a simpler queueing network amenable to solution via algorithms based on mean value analysis (MVA) for product-form networks.FindingsNumerical results justify the proposed approach for getting a fast, yet accurate analytical solution that allows carrying out performance evaluation with respect to both organizational policies and operations management on the yard area.Practical implicationsPractically, the expected performance measures on the yard subsystem can be obtained avoiding additional time-expensive simulation experiments on the entire detailed model.Originality/valueAs a major takeaway, deepening the MVA for generally distributed service times has proven to produce reliable estimations on expected values for both user- and system-oriented performance metrics.
Impact of major stock markets on China's stock market
Authors: Hull Robert M. , Habib Ashfaq , Khan Muhammad Asif 
Published: May 2023

PurposeThe main purpose is to explore the impact of major stock markets on China's market where major markets are represented by former G8 nations (current G7 and Russia).Design/methodology/approachThe article makes use of: stationarity tests (ADF and PP unit root); long-run correlation tests (Johansen integration involving trace and maximum eigenvalue); impact of G8 markets on China (VECM test); influence of G8 markets on volatility in China's market (variance decomposition analysis) and, effect from shocks in G8 markets on China (impulse response function).FindingsUsing a period of 2009–2019 that avoids detecting linkages caused by interdependencies created by two major international crises, the article offers four major findings. First, except for Germany and Russia, G8 markets have a significant causal influence on China with UK having the greatest. Second, G8 markets are not the major source of short-run fluctuation in China's market but over time exercise a noteworthy collective impact with UK having the greatest impact. Third, there are occasions for international portfolio diversification with China's market providing greater diversification than G8 nations. Fourth, all markets provide a short-run window of abnormal profit.Research limitations/implicationsThe indexes used to represent national markets are assumed to be adequate representations.Practical implicationsShort-term abnormal profits exist. Investing in China, compared to G8 countries, offers greater portfolio diversification possibilities.Social implicationsRemoval of trade and investment barriers cause greater market integration.Originality/valueBy using recent data, this study reveals that G8 stock markets influence China's market.
Screening for silent aspiration in hyperacute stroke: A feasibility study of clinical swallowing examination and cough reflex testing
Authors: Trimble Julie , Patterson Joanne M. , Wilson Janet A. , Dixit Anand K. , Drinnan Michael 
Published: May 2023

BackgroundSilent aspiration (SA) is common post‐stroke and associated with increased risk of pneumonia, length of stay and healthcare costs. Clinical swallow examinations (CSEs) are unreliable measures of SA. There is no consensus on the clinical components that best detect SA. Cough reflex testing (CRT) is an alternative/adjunct whose SA detection accuracy also lacks consensus.AimsTo investigate the feasibility of CSE versus CRT against gold standard flexible endoscopic evaluation of swallowing (FEES) for SA identification and to estimate its prevalence in a hyperacute stroke setting.Methods & ProceduresA single‐arm preliminary, prospective, feasibility study of patients less than 72 h post‐stroke, over a 31‐day period on a hyperacute stroke unit: the Royal Victoria Infirmary, Newcastle‐upon‐Tyne, UK. Ethical approval for the study was obtained. The study tested the feasibility and acceptability of introducing CRT and developing a standardized CSE. Consent/assent was obtained for all participants. Patients unfit for study were excluded.Outcomes & ResultsA total of 62% of patients less than 72 h post‐stroke (n = 61) were eligible. A total of 75% of those approached (n = 30) consented. A total of 23 patients completed all tests. The principal barrier was anxiety regarding FEES. Mean test time for CRT = 6 min; CSE = 8 min; FEES = 17 min. Patients rated CRT and FEES on average as moderately uncomfortable. A total of 30% (n = 7) of participants who received FEES presented with SA.Conclusions & ImplicationsCRT, CSE and FEES are feasible in 58% of hyperacute stroke patients in this setting. FEES anxiety is the main recruitment barrier and is not always well tolerated. Results support further work to establish optimum methods and differential sensitivity/specificity of CRT and CSE in hyperacute stroke for SA identification.WHAT THIS PAPER ADDSWhat is already known on this subject
SA significantly increases the risk of pneumonia in the early days post‐stroke. CSEs are unreliable for identification of SA risk in this population. CRT is gaining popularity as a potential tool to identify stroke patients at risk of SA, though there are questions regarding the efficacy of the clinical protocol currently being used in the UK.What this study adds to existing knowledge
This study demonstrates that it is practical and feasible to carry out a larger scale study in this setting to compare CSE and CRT including a consideration of an approach combining both methods for clinical identification of SA versus FEES. Preliminary findings suggest that CSE may have higher levels of sensitivity than CRT for SA identification.What are the potential or actual clinical implications of this work?
The results of this study suggest that further work is needed to establish the optimum methods and differential sensitivity/specificity of clinical tools for SA detection in hyperacute stroke.
Spatial analysis of future climate risk to stormwater infrastructure
Authors: Butcher Jonathan B. , Sarkar Saumya , Johnson Thomas E. , Shabani Afshin 
Published: May 2023

AbstractClimate change is expected to result in more intense precipitation events that will affect the performance and design requirements of stormwater infrastructure. Such changes will vary spatially, and climate models provide a range of estimates of the effects on events of different intensities and recurrence. Infrastructure performance should be evaluated against the expected range of events, not just rare extremes. We present a national‐scale, spatially detailed screening assessment of the potential effects of climatic change on precipitation, stormwater runoff, and associated design requirements. This is accomplished through adjustment relative to multiple future climate scenarios of precipitation intensity–duration–frequency analyses presented in NOAA Atlas 14, which are commonly used in infrastructure design. Future precipitation results are estimated for each Atlas 14 station (these currently omit the Pacific Northwest). Results are interpolated using a geographically conditioned regression kriging approach to provide information about potential climate change impacts in a format more directly useful to local stormwater managers. The intensity of 24‐h events with 2‐year or greater recurrence is likely to increase in most areas of the United States leading to increased runoff and potential need for increased storage volumes. Changes in more frequent events (e.g., the 90th percentile event) commonly used in design of green infrastructure are relatively less.
Autoimmune diseases post‐COVID 19 infection in children in intensive care unit: A case series
Authors: Das Subhadipa , Parul  , Samanta Moumita 
Published: May 2023

AbstractSARS‐CoV2 primarily affects the respiratory system but a hyperinflammatory response leading to multisystem inflammatory syndrome – children (MIS‐C), immune dysfunction and various autoimmune manifestations has also been noted. Autoimmunity depends on various factors, including genetic predisposition, environmental factors, immune dysregulation and infections acting as triggers like Epstein‐Barr virus, cytomegalovirus, human immunodeficiency virus, hepatitis B. Molecular mimicry, bystander T‐cell activation and persistence of viral infection are the main mechanisms behind these manifestations. We present here 3 cases of newly diagnosed connective tissue disease with high titers of COVID19 immunoglobulin G antibody in children. A 9‐year‐old girl with fever, oliguria and malar rash (prior history of sore throat) and a 10‐year‐old girl with fever for 2 weeks and choreoathetoid movements were diagnosed as systemic lupus erythematosus (SLE) nephritis (stage 4) and neuropsychiatric SLE, respectively as per European League Against Rheumatism / American College of Rheumatology 2019 criteria. An 8‐year‐old girl with fever, joint pain and respiratory distress (a recent contact with a positive COVID19 patient) presented with altered sensorium, Raynaud's phenomenon noted, and eventually diagnosed as mixed connective tissue disease as per Kusukawa criteria. The immune‐mediated manifestations post‐COVID infection are a de‐novo phenomenon which necessitates further workup as not many studies exist in the pediatric population.
Spatial resolution of cellular senescence dynamics in human colorectal liver metastasis
Authors: Garbarino Ombretta , Lambroia Luca , Basso Gianluca , Marrella Veronica , Franceschini Barbara , Soldani Cristiana , Pasqualini Fabio , Giuliano Desiree , Costa Guido , Peano Clelia , Barbarossa Davide , Annarita Destro , Salvati Andreina , Terracciano Luigi , Torzilli Guido , Donadon Matteo , Faggioli Francesca 
Published: May 2023

AbstractHepatic metastasis is a clinical challenge for colorectal cancer (CRC). Senescent cancer cells accumulate in CRC favoring tumor dissemination. Whether this mechanism progresses also in metastasis is unexplored. Here, we integrated spatial transcriptomics, 3D‐microscopy, and multicellular transcriptomics to study the role of cellular senescence in human colorectal liver metastasis (CRLM). We discovered two distinct senescent metastatic cancer cell (SMCC) subtypes, transcriptionally located at the opposite pole of epithelial (e) to mesenchymal (m) transition. SMCCs differ in chemotherapy susceptibility, biological program, and prognostic roles. Mechanistically, epithelial (e)SMCC initiation relies on nucleolar stress, whereby c‐myc dependent oncogene hyperactivation induces ribosomal RPL11 accumulation and DNA damage response. In a 2D pre‐clinical model, we demonstrated that RPL11 co‐localized with HDM2, a p53‐specific ubiquitin ligase, leading to senescence activation in (e)SMCCs. On the contrary, mesenchymal (m)SMCCs undergo TGFβ paracrine activation of NOX4‐p15 effectors. SMCCs display opposing effects also in the immune regulation of neighboring cells, establishing an immunosuppressive environment or leading to an active immune workflow. Both SMCC signatures are predictive biomarkers whose unbalanced ratio determined the clinical outcome in CRLM and CRC patients. Altogether, we provide a comprehensive new understanding of the role of SMCCs in CRLM and highlight their potential as new therapeutic targets to limit CRLM progression.