bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of social support and sociocultural adjustment for international students' mental health
Authors: Razgulin Jevgenij , Argustaitė-Zailskienė Gita , Šmigelskas Kastytis 
Published: January 2023

AbstractThe study aims to assess the role of social support, sociocultural adjustment, and other social and demographic factors in international students' mental health. In total, 193 international first-year students studying Health and Technology Sciences in Lithuania filled out a self-report questionnaire. The study revealed that overall 59% of international students had symptoms of depression and 36%—of anxiety. Students' well-being was sufficient in 56% of the cases. The regression analyses were conducted to test the role of sociocultural adjustment, social support, English reading skills, and the study field for mental health and well-being. The multivariate regression model revealed that sociocultural adjustment is a stronger predictor compared to social support for depressiveness (βs = 0.42), anxiety (βs = 0.30), psychosomatic symptoms (βs = − 0.24), and subjective health (βs = 0.16), though social support was a stronger independent predictor for well-being (βs = 0.37). Moreover, sociocultural adjustment and social support were stronger predictors than gender, while English reading skills and study field were non-significant indicators.
Modeling marine cargo traffic to identify countries in Africa with greatest risk of invasion by Anopheles stephensi
Authors: Ahn Jordan , Sinka Marianne , Irish Seth , Zohdy Sarah 
Published: January 2023

AbstractAnopheles stephensi, an invasive malaria vector native to South Asia and the Arabian Peninsula, was detected in Djibouti’s seaport, followed by Ethiopia, Sudan, Somalia, and Nigeria. If An. stephensi introduction is facilitated through seatrade, similar to other invasive mosquitoes, the identification of at-risk countries are needed to increase surveillance and response efforts. Bilateral maritime trade data is used to (1) identify coastal African countries which were highly connected to select An. stephensi endemic countries, (2) develop a prioritization list of countries based on the likelihood of An. stephensi introduction through maritime trade index (LASIMTI), and (3) use network analysis of intracontinental maritime trade to determine likely introduction pathways. Sudan and Djibouti were ranked as the top two countries with LASIMTI in 2011, which were the first two coastal African countries where An. stephensi was detected. With Djibouti and Sudan included as source populations, 2020 data identify Egypt, Kenya, Mauritius, Tanzania, and Morocco as the top countries with LASIMTI. Network analysis highlight South Africa, Mauritius, Ghana, and Togo. These tools can prioritize efforts for An. stephensi surveillance and control in Africa. Surveillance in seaports of identified countries may limit further expansion of An. stephensi by serving as an early warning system.
Exploring the mobility in the Madrid Community
Authors: Mouronte-López Mary Luz , Gómez Javier 
Published: January 2023

AbstractDisplacements within urban spaces have attracted particular interest among researchers. We examine the journeys that happen in the Madrid Community considering 24 travel typologies and 1390 administrative areas. From an origin–destination (OD) matrix, four classes of major flows are characterised through coarse-graining: hotspot–non-hotspots, non-hotspot–hotspots, hotspots–hotspots, non-hotspot–non-hotspot. In order to make comparisons between them with respect to spatial and temporal patterns, several statistical tests are performed. The spatial activity as well as transition probabilities between administrative zones are also analysed. The mobility network’s topology is examined (some parameters such as maximal connected components, average degree, betweenness, and assortativity as well as the k-cores are checked). A model describing the formation of links between zones (existence of at least one trip between them) is constructed based on certain measures of affinity between areas.
Vasoactive intestinal peptide blockade suppresses tumor growth by regulating macrophage polarization and function in CT26 tumor-bearing mice
Authors: Kittikulsuth Wararat , Nakano Daisuke , Kitada Kento , Uyama Toru , Ueda Natsuo , Asano Eisuke , Okano Keiichi , Matsuda Yoko , Nishiyama Akira 
Published: January 2023

AbstractMacrophages are a major population of immune cells in solid cancers, especially colorectal cancers. Tumor-associated macrophages (TAMs) are commonly divided into M1-like (tumor suppression) and M2-like (tumor promotion) phenotypes. Vasoactive intestinal peptide (VIP) is an immunoregulatory neuropeptide with a potent anti-inflammatory function. Inhibition of VIP signaling has been shown to increase CD8+ T cell proliferation and function in viral infection and lymphoma. However, the role of VIP in macrophage polarization and function in solid tumors remains unknown. Here, we demonstrated that conditioned medium from CT26 (CT26-CM) cells enhanced M2-related marker and VIP receptor (VPAC) gene expression in RAW264.7 macrophages. VIP hybrid, a VIP antagonist, enhanced M1-related genes but reduced Mrc1 gene expression and increased phagocytic ability in CT26-CM-treated RAW264.7 cells. In immunodeficient SCID mice, VIP antagonist alone or in combination with anti-PD-1 antibody attenuated CT26 tumor growth compared with the control. Analysis of tumor-infiltrating leukocytes found that VIP antagonist increased M1/M2 ratios and macrophage phagocytosis of CT26-GFP cells. Furthermore, Vipr2 gene silencing or VPAC2 activation affected the polarization of CT26-CM-treated RAW264.7 cells. In conclusion, the inhibition of VIP signaling enhanced M1 macrophage polarization and macrophage phagocytic function, resulting in tumor regression in a CT26 colon cancer model.
Efficient adsorptive removal of paracetamol and thiazolyl blue from polluted water onto biosynthesized copper oxide nanoparticles
Authors: Akpomie Kovo G. , Conradie Jeanet 
Published: January 2023

AbstractCopper oxide nanoparticles (CuONPs) have received tremendous attention as efficient adsorbents owing to their low cost, desirable surface area, abundant active sites, potent textural characteristics and high adsorption capacities. However, CuONPs have not been employed to decontaminate water laden with increasing environmental contaminants such as thiazolyl blue and paracetamol. Herein, the adsorption of thiazolyl blue and paracetamol onto green synthesized CuONPs prepared from the aqueous leaf extract of Platanus occidentalis was studied. The BET, SEM, FTIR, XRD, EDX and pH point of zero charge showed the successful synthesis of CuONPs having desirable surface properties with a surface area of 58.76 m2/g and an average size of 82.13 nm. The maximum monolayer adsorption capacities of 72.46 mg/g and 64.52 mg/g were obtained for thiazolyl blue and paracetamol, respectively. The Freundlich, pseudo-second-order and intraparticle diffusion models were well fitted to the adsorption of both pollutants. The pH studies suggested the predominance of electrostatic and weaker intermolecular interactions in the adsorption of the thiazolyl blue and paracetamol, respectively. Spontaneous, physical, endothermic and random adsorption of the pollutants on CuONPs was obtained from the thermodynamic consideration. The biosynthesized CuONPs were found to be highly reusable and efficient for the adsorption of thiazolyl blue and paracetamol from water.
Impact of iron status on kidney outcomes in kidney transplant recipients
Authors: Kim Hyo Jeong , Han Ro , Kang Kyung Pyo , Ryu Jung-Hwa , Kim Myung-Gyu , Huh Kyu Ha , Park Jae Berm , Kim Chan-Duck , Han Seungyeup , Kim Hyung Woo , Kim Beom Seok , Yang Jaeseok 
Published: January 2023

AbstractIron plays an important role in hemodynamics and the immunity, independent of anemia. Since dynamic changes occur in iron storage after kidney transplantation (KT), we investigated the association between iron status and kidney outcomes in KT patients. We analyzed data from the KoreaN cohort study for Outcome in patients With KT (KNOW-KT). The iron status was classified into three groups based on ferritin or transferrin saturation (TSAT) levels one year after KT, with reference ranges of 20‒35% and 100‒300 ng/mL for TSAT and ferritin, respectively. The primary outcome was the composite outcome, which consisted of death, graft failure, and an estimated glomerular filtration rate decline ≥ 50%. In total, 895 patients were included in the final analysis. During a median follow-up of 5.8 years, the primary outcome occurred in 94 patients (19.8/1000 person-years). TSAT levels decreased one year after KT and thereafter gradually increased, whereas ferritin levels were maintained at decreased levels. The adjusted hazard ratios (95% confidence intervals) for the composite outcome were 1.67 (1.00–2.77) and 1.20 (0.60–2.40) in the TSAT > 35% and ferritin > 300 ng/mL groups, respectively. High iron status with high TSAT levels increases the risk of graft failure or kidney functional deterioration after KT.
Distinctive physical insights driven from machine learning modelling of nuclear power plant severe accident scenario propagation
Authors: Hossny K. , Villanueva W. , Wang H. D. 
Published: January 2023

AbstractThe severe accident scenario propagation studies of nuclear power plants (NPPs) have been one of the most critical factors in deploying nuclear power for decades. During an NPP accident, the accident scenario can change during its propagation from the initiating event to a series of accident sub-scenarios. Hence, having time-wise updated information about the current type of accident sub-scenario can help plant operators mitigate the accident propagation and underlying consequences. In this work, we demonstrate the capability of machine learning (Decision Tree) to help researchers and design engineers in finding distinctive physical insights between four different types of accident scenarios based on the pressure vessel's maximum external surface temperature at a particular time. Although the four accidents we included in this study are considered some of the most extensively studied NPPs accident scenarios for decades, our findings shows that decision tree classification could define remarkable distinct differences between them with reliable statistical confidence.
Deep reptilian evolutionary roots of a major avian respiratory adaptation
Authors: Wang Yan-yin , Claessens Leon P. A. M. , Sullivan Corwin 
Published: January 2023

AbstractVertebral ribs of the anterior thorax in extant birds bear bony prongs called uncinate processes, which improve the mechanical advantage of mm. appendicocostales to move air through the immobile lung and pneumatic air sacs. Among non-avian archosaurs, broad, cartilaginous uncinate processes are present in extant crocodylians, and likely have a ventilatory function. Preserved ossified or calcified uncinate processes are known in several non-avian dinosaurs. However, whether other fossil archosaurs possessed cartilaginous uncinate processes has been unclear. Here, we establish osteological correlates for uncinate attachment to vertebral ribs in extant archosaurs, with which we inferred the presence of uncinate processes in at least 19 fossil archosaur taxa. An ancestral state reconstruction based on the infer distribution suggests that cartilaginous uncinate processes were plesiomorphically present in Dinosauria and arguably in Archosauria, indicating that uncinate processes, and presumably their ventilatory function, have a deep evolutionary history extending back well beyond the origin of birds.
Role of miR-944/MMP10/AXL- axis in lymph node metastasis in tongue cancer
Authors: Dharavath Bhasker , Butle Ashwin , Pal Ankita , Desai Sanket , Upadhyay Pawan , Rane Aishwarya , Khandelwal Risha , Manavalan Sujith , Thorat Rahul , Sonawane Kavita , Vaish Richa , Gera Poonam , Bal Munita , D’Cruz Anil K. , Nair Sudhir , Dutt Amit 
Published: January 2023

AbstractOccult lymph-node metastasis is a crucial predictor of tongue cancer mortality, with an unmet need to understand the underlying mechanism. Our immunohistochemical and real-time PCR analysis of 208 tongue tumors show overexpression of Matrix Metalloproteinase, MMP10, in 86% of node-positive tongue tumors (n = 79; p < 0.00001). Additionally, global profiling for non-coding RNAs associated with node-positive tumors reveals that of the 11 significantly de-regulated miRNAs, miR-944 negatively regulates MMP10 by targeting its 3’-UTR. We demonstrate that proliferation, migration, and invasion of tongue cancer cells are suppressed by MMP10 knockdown or miR-944 overexpression. Further, we show that depletion of MMP10 prevents nodal metastases using an orthotopic tongue cancer mice model. In contrast, overexpression of MMP10 leads to opposite effects upregulating epithelial-mesenchymal-transition, mediated by a tyrosine kinase gene, AXL, to promote nodal and distant metastasis in vivo. Strikingly, AXL expression is essential and sufficient to mediate the functional consequence of MMP10 overexpression. Consistent with our findings, TCGA-HNSC data suggests overexpression of MMP10 or AXL positively correlates with poor survival of the patients. In conclusion, our results establish that the miR-944/MMP10/AXL- axis underlies lymph node metastases with potential therapeutic intervention and prediction of nodal metastases in tongue cancer patients.
Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria
Authors: Li Chenhao , Wang Longfei , Cseke Leland J. , Vasconcelos Fernanda , Huguet-Tapia Jose Carlos , Gassmann Walter , Pauwels Laurens , White Frank F. , Dong Hansong , Yang Bing 
Published: January 2023

AbstractPhytopathogenic bacteria play important roles in plant productivity, and developments in gene editing have potential for enhancing the genetic tools for the identification of critical genes in the pathogenesis process. CRISPR-based genome editing variants have been developed for a wide range of applications in eukaryotes and prokaryotes. However, the unique mechanisms of different hosts restrict the wide adaptation for specific applications. Here, CRISPR-dCas9 (dead Cas9) and nCas9 (Cas9 nickase) deaminase vectors were developed for a broad range of phytopathogenic bacteria. A gene for a dCas9 or nCas9, cytosine deaminase CDA1, and glycosylase inhibitor fusion protein (cytosine base editor, or CBE) was applied to base editing under the control of different promoters. Results showed that the RecA promoter led to nearly 100% modification of the target region. When residing on the broad host range plasmid pHM1, CBERecAp is efficient in creating base edits in strains of Xanthomonas, Pseudomonas, Erwinia and Agrobacterium. CBE based on nCas9 extended the editing window and produced a significantly higher editing rate in Pseudomonas. Strains with nonsynonymous mutations in test genes displayed expected phenotypes. By multiplexing guide RNA genes, the vectors can modify up to four genes in a single round of editing. Whole-genome sequencing of base-edited isolates of Xanthomonas oryzae pv. oryzae revealed guide RNA-independent off-target mutations. Further modifications of the CBE, using a CDA1 variant (CBERecAp-A) reduced off-target effects, providing an improved editing tool for a broad group of phytopathogenic bacteria.
Directly imaging emergence of phase separation in peroxidized lipid membranes
Authors: Paez-Perez Miguel , Vyšniauskas Aurimas , López-Duarte Ismael , Lafarge Eulalie J. , López-Ríos De Castro Raquel , Marques Carlos M. , Schroder André P. , Muller Pierre , Lorenz Christian D. , Brooks Nicholas J. , Kuimova Marina K. 
Published: January 2023

AbstractLipid peroxidation is a process which is key in cell signaling and disease, it is exploited in cancer therapy in the form of photodynamic therapy. The appearance of hydrophilic moieties within the bilayer’s hydrocarbon core will dramatically alter the structure and mechanical behavior of membranes. Here, we combine viscosity sensitive fluorophores, advanced microscopy, and X-ray diffraction and molecular simulations to directly and quantitatively measure the bilayer’s structural and viscoelastic properties, and correlate these with atomistic molecular modelling. Our results indicate an increase in microviscosity and a decrease in the bending rigidity upon peroxidation of the membranes, contrary to the trend observed with non-oxidized lipids. Fluorescence lifetime imaging microscopy and MD simulations give evidence for the presence of membrane regions of different local order in the oxidized membranes. We hypothesize that oxidation promotes stronger lipid-lipid interactions, which lead to an increase in the lateral heterogeneity within the bilayer and the creation of lipid clusters of higher order.
Singlet fission dynamics modulated by molecular configuration in covalently linked pyrene dimers, Anti- and Syn-1,2-di(pyrenyl)benzene
Authors: Choi Jungkweon , Kim Siin , Ahn Mina , Kim Jungmin , Cho Dae Won , Kim Doyeong , Eom Seunghwan , Im Donghwan , Kim Yujeong , Kim Sun Hee , Wee Kyung-Ryang , Ihee Hyotcherl 
Published: January 2023

AbstractCovalently linked dimers (CLDs) and their structural isomers have attracted much attention as potential materials for improving power conversion efficiencies through singlet fission (SF). Here, we designed and synthesized two covalently ortho-linked pyrene (Py) dimers, anti- and syn-1,2-di(pyrenyl)benzene (Anti-DPyB and Syn-DPyB, respectively), and investigated the effect of molecular configuration on SF dynamics using steady-state and time-resolved spectroscopies. Both Anti-DPyB and Syn-DPyB, which have different Py-stacking configurations, form excimers, which then relax to the correlated triplet pair ((T1T1)) state, indicating the occurrence of SF. Unlike previous studies where the excimer formation inhibited an SF process, the (T1T1)’s of Anti-DPyB and Syn-DPyB are formed through the excimer state. The dissociation of (T1T1)’s to 2T1 in Anti-DPyB is more favorable than in Syn-DPyB. Our results showcase that the molecular configuration of a CLD plays an important role in SF dynamics.
Metal-Free Cascade Synthesis of 1,2,4-Thiadiazoles from β-Ketothioamides and Alkyl/Aryl Cyanides under TBN Enabling Conditions at Room Temperature
Authors: Singh Maya Shankar , Singh Saurabh , Shukla Gaurav , Ray Subhasish 
Published: January 2023

AbstractA metal-free radical cascade cyclization of β-ketothioamides and alkyl/aryl cyanides has been developed for the synthesis of 1,2,4-thiadiazoles bearing a stable nitroso group at an exocyclic double bond in a one-pot system. The reaction proceeds via the in situ generated ketoxime intermediate of β-ketothioamide, which further assists the formation of 1,2,4-thiadiazole through reaction with nitrile under TBN (tert-butyl nitrite) mediation. The basic utility of this reaction is the formation of three new bonds (two C–N and one S–N) in one stretch under mild conditions. Moreover, this protocol features excellent functional group tolerance, operational simplicity, and easy scalability. The radical process is supported by the BHT adduct of the α-ketoxime intermediate. Noteworthy is that the products possess Z stereochemistry with regard to the exocyclic C=C double bond at the 5-position.
Language and Literacy Strategies for Indigenous Children: A Scoping Review
Authors: Guiberson Mark , Vining Christine B. 
Published: January 2023

AbstractThe purpose of this scoping review was to describe the existent research on language and literacy strategies for Indigenous children and to establish the strength of strategies described. A scoping review was conducted to locate existent studies that described language and literacy interventions used with Indigenous children. The following electronic databases were searched: PsycINFO, ERIC, CINAHL Complete, Academic Search Premier, Education Source, and ASHAWire. Articles were managed and analyzed using Covidence, a web-based program for review research. Results were charted and a preliminary evidence map was created. Forty sources were identified that described language and literacy strategies for Indigenous children. Strength of strategy coding revealed 5 sources had compelling strength, 5 had promising strength, and 30 had lacking strength. Overall, there remains limited research describing language and literacy strategies for Indigenous children. A preliminary evidence map was created to chart each strategy and sources that included the strategy, and to indicate the highest strategy strength observed across sources. A discussion of compelling strategies as well as strategies that may be culturally responsive is provided.
Cognitive Profile in Patients Admitted to Intensive Rehabilitation after Stroke Is Associated with the Recovery of Dysphagia: Preliminary Results from the RIPS (Intensive Post-Stroke Rehabilitation) Study
Authors: Basagni Benedetta , Marignani Sara , Pancani Silvia , Mannini Andrea , Hakiki Bahia , Grippo Antonello , Macchi Claudio , Cecchi Francesca 
Published: January 2023

AbstractDysphagia represents one of the most frequent symptoms in the post–acute stroke population. Swallowing impairment and cognitive deficits can often co-occur. This study aims to investigate the relationship between cognitive impairment and the recovery of dysphagia in patients attending specific rehabilitation. Patients admitted to intensive rehabilitation units were administered the Functional Oral Intake Scale (FOIS) and Montreal Cognitive Screening Test (MoCA); when screening positive for dysphagia, they entered a rehabilitation program. Their FOIS score at discharge was the primary outcome measure. In the multivariate analysis, younger age (B = − 0.077, p = 0.017), higher MoCA (B = 0.191, p = 0.002), and higher FOIS (B = 1.251, p = 0.032) at admission were associated with higher FOIS at discharge. When executive function (EF) replaced the MoCA total score in the model, younger age (B = − 0.134, p = 0.001), higher admission EF (B = 1.451, p < 0.001), and FOIS (B = 1.348, p = 0.035) were associated with higher FOIS at discharge. Our results confirm the hypothesis that a better cognitive profile upon admission is associated with a higher probability of dysphagia recovery at discharge. EF seems to have a crucial role in dysphagia recovery. These results highlight the importance of considering the cognitive profile when assessing and treating dysphagia after stroke and of using screening tests that include executive functions.
Using heparan sulfate octadecasaccharide (18-mer) as a multi-target agent to protect against sepsis
Authors: Liao Yi-En , Xu Yongmei , Arnold Katelyn , Zhang Fuming , Li Jine , Sellers Rani , Yin Chaoyin , Pagadala Vijayakanth , Inman Anna Marie , Linhardt Robert J. , Xu Ding , Pawlinski Rafal , Liu Jian 
Published: January 2023

Sepsis is a lethal syndrome manifested by an unregulated, overwhelming inflammation from the host in response to infection. Here, we exploit the use of a synthetic heparan sulfate octadecasaccharide (18-mer) to protect against sepsis. The 18-mer not only inhibits the pro-inflammatory activity of extracellular histone H3 and high mobility group box 1 (HMGB1), but also elicits the anti-inflammatory effect from apolipoprotein A-I (ApoA-I). We demonstrate that the 18-mer protects against sepsis-related injury and improves survival in cecal ligation and puncture mice and reduces inflammation in an endotoxemia mouse model. The 18-mer neutralizes the cytotoxic histone-3 (H3) through direct interaction with the protein. Furthermore, the 18-mer enlists the actions of ApoA-I to dissociate the complex of HMGB1 and lipopolysaccharide, a toxic complex contributing to cell death and tissue damage in sepsis. Our study provides strong evidence that the 18-mer mitigates inflammatory damage in sepsis by targeting numerous mediators, setting it apart from other potential therapies with a single target.