bluuebunny/crossref_metadata_2025_split huggingface dataset:
Round-robin testing for LMO2 and MYC as immunohistochemical markers to screen MYC rearrangements in aggressive large B-cell lymphoma
Authors: Papaleo Natalia , Climent Fina , Tapia Gustavo , Luizaga Luis , Azcarate Juan , Bosch-Schips Jan , Muñoz-Marmol Ana M. , Salido Marta , Lome-Maldonado Carmen , Vazquez Ivonne , Colomo Luis 
Published: June 2023

AbstractAggressive large B-cell lymphomas (aLBCL) include a heterogeneous group of lymphomas with diverse biological features. One of the approaches to the diagnosis of aLBCL is based on the identification of MYC rearrangements (MYC-R), in addition to BCL2 and BCL6 rearrangements by genetic techniques, mainly fluorescent in situ hybridization (FISH). Because of the low incidence of MYC-R, the identification of useful immunohistochemistry markers to select cases for MYC FISH testing may be useful in daily practice. In a previous work, we identified a strong association between the profile CD10 positive/LMO2 negative expression and the presence of MYC-R in aLBCL and obtained good intralaboratory reproducibility. In this study, we wanted to evaluate external reproducibility. To evaluate whether LMO2 can be a reproducible marker between observers 50 aLBCL cases were circulated among 7 hematopathologists of 5 hospitals. Fleiss’ kappa index for LMO2 and MYC were 0.87 and 0.70, respectively, indicating high agreement between observers. In addition, during 2021–2022, the enrolled centers included LMO2 in their diagnostic panels to evaluate prospectively the utility of the marker, and 213 cases were analyzed. Comparing LMO2 with MYC, the group of CD10 positive cases showed higher specificity (86% vs 79%), positive predictive value (66% vs 58%), likelihood positive value (5.47 vs 3.78), and accuracy (83% vs 79%), whereas the negative predictive values remained similar (90% vs 91%). These findings place LMO2 as a useful and reproducible marker to screen MYC-R in aLBCL.
The long weight: association between distressed communities index and long-term weight outcomes following bariatric surgery
Authors: Johns Alexandra J. , Luce M. Siobhan , Kaneski Mason J. , Lowery Ryan A. , Jachniewicz Barbara , Salas Angela , McCreary Randi , Russell Raquel M. , Lyo Victoria , Ali Mohammed R. , Ahmed Shushmita M. 
Published: June 2023

Abstract
Background
Socioeconomic status (SES) is multifactorial, and its effect on post-bariatric weight recurrence is unclear. Distressed Community Index (DCI) is a composite SES score measuring community economic well-being. This study aims to evaluate the effect of DCI on long-term post-bariatric weight outcomes.

Methods
Retrospective analysis of patients undergoing primary laparoscopic Roux-en-Y gastric bypass or sleeve gastrectomy between 2015 and 2020 was performed. All weights in the electronic medical record (EMR), including non-bariatric visits, were captured. Patients were stratified into low tier (LT) and high tier (HT) DCI groups.

Results
Of 583 patients, 431 (73.9%) were HT and 152 (26.1%) were LT. Average bariatric follow up was 1.78 ± 1.6 years and average postoperative weight in the EMR was 3.96 ± 2.26 years. Rates of bariatric follow up within the last year were similar (13.8% LT vs 16.2% HT, p = 0.47). LT had higher percent total body weight loss (%TWL; 26% LT vs 23% HT, p < 0.01) and percent excess weight loss (%EWL; 62% vs 57%, p = 0.04) at 1 year on univariate analysis. On multivariate linear regression adjusting for baseline characteristics and surgery type, there were no differences in %EWL between groups at 1 year (p = 0.22), ≥ 3 years (p = 0.53) or ≥ 5 years (p = 0.34) postop. While on univariate analysis LT only trended towards greater percentage of patients with > 15% increase from their 1-year weight (33.3% LT vs 21.0% HT, p = 0.06), on multivariate analysis this difference was significant (OR 2.0, LT 95%CI 1.41–2.84). There were no differences in the percentage of patients with > 15% decrease in %EWL from 1 to 3 + years postop between groups (OR 0.98, LT 95% CI 0.72–1.35).

Conclusions
While low tier patients had similar weight loss at 1 year, they were twice as likely to have weight recurrence at ≥ 3 years. Further studies are needed to identify factors contributing to greater weight recurrence among this population.
Phase II clinical trial to study the safety and efficacy of combined S-1 + oxaliplatin therapy as neoadjuvant chemotherapy for locally advanced gastric cancer in older patients
Authors: Ota Mitsuhiko , Saeki Hiroshi , Uehara Hideo , Matsuda Yoshiko , Tsutsumi Satoshi , Kusumoto Tetsuya , Yasui Hisateru , Ubukata Yasunari , Yamaguchi Shohei , Orita Hiroyuki , Izawa Naoki , Kakizoe Saburo , Shimokawa Mototsugu , Yoshizumi Tomoharu , Kakeji Yoshihiro , Mori Masaki , Oki Eiji 
Published: June 2023

Abstract
Background
Gastrectomy with D2 dissection and adjuvant chemotherapy is the standard treatment for locally advanced gastric cancer (LAGC) in Asia. However, administering chemotherapy with sufficient intensity after gastrectomy is challenging. Several trials demonstrated the efficacy of neoadjuvant chemotherapy (NAC). However, limited studies explored the feasibility of NAC-SOX for older patients with LAGC. This phase II study (KSCC1801) evaluated the safety and efficacy of NAC-SOX in patients with LAGC aged ≥ 70 years.

Methods
Patients received three cycles of SOX130 (oxaliplatin 130 mg/m2 on day 1, oral S-1 40–60 mg twice daily for two weeks every three weeks) as NAC, followed by gastrectomy with lymph node dissection. The primary endpoint was the dose intensity (DI). The secondary endpoints were safety, R0 resection rate, pathological response rate (pRR), overall survival, and relapse-free survival.

Results
The median age of 26 enrolled patients was 74.5 years. The median DI in NAC-SOX130 was 97.2% for S-1 and 98.3% for oxaliplatin. Three cycles of NAC were administered in 25 patients (96.2%), of whom 24 (92.3%) underwent gastrectomy with lymphadenectomy. The R0 resection rate was 92.3% and the pRR (≥ grade 1b) was 62.5%. The major adverse events (≥ grade 3) were neutropenia (20.0%), thrombocytopenia (11.5%), anorexia (11.5%), nausea (7.7%), and hyponatremia (7.7%). Postoperative complications of abdominal infection, elevated blood amylase, and bacteremia occurred in one patient each. Severe diarrhea and dehydration caused one treatment-related death.

Conclusions
NAC-SOX130 is a feasible therapy for older patients, although systemic management and careful monitoring of adverse events are necessary.
The policy is always greener: impact heterogeneity of Covid-19 vaccination lotteries in the US.
Authors: Grossi Giulio 
Published: June 2023

AbstractCovid-19 vaccination has posed crucial challenges to policymakers and health administrations worldwide. Besides the pressure posed by the pandemic, government administrations have to strive against vaccine hesitancy, which seems to be higher with respect to previous vaccination rollouts. To increase the vaccinated population, Ohio announced a monetary incentive as a lottery for those who were vaccinated. 18 other states followed this first example, with varying results. In this paper, we want to evaluate the effect of such policies within the potential outcome framework using the penalized synthetic control method. In the context of staggered treatment adoption, we estimate the effects at a disaggregated level using a panel dataset. We focused on policy outcomes at the county, state, and supra-state levels, highlighting differences between counties with different social characteristics and time frames for policy introduction. We also studied the treatment effect to see whether the impact of these monetary incentives was permanent or only temporary, accelerating the vaccination of citizens who would have been vaccinated in any case.
Phylogenetic analyses of antimicrobial resistant Corynebacterium striatum strains isolated from a nosocomial outbreak in a tertiary hospital in China
Authors: Li Yuchuan , Rong Jianrong , Gao Chunyan 
Published: June 2023

AbstractCorynebacterium striatum is an emerging, multidrug-resistant pathogen that frequently causes nosocomial infections worldwide. This study aimed to investigate phylogenetic relationship and presence of genes responsible for antimicrobial resistance among C. striatum strains associated with an outbreak at the Shanxi Bethune Hospital, China, in 2021. Fecal samples were collected from 65 patients with C. striatum infection at Shanxi Bethune Hospital between February 12, 2021 and April 12, 2021. C. striatum isolates were identified by 16S rRNA and rpoB gene sequencing. E-test strips were used to examine the antimicrobial susceptibility of the isolates. Whole-genome sequencing and bioinformatics analysis were employed to assess the genomic features and identify antimicrobial resistance genes of the isolates. Crystal violet staining was conducted to determine the ability of biofilm formation of each isolate. A total of 64 C. striatum isolates were identified and categorized into 4 clades based on single nucleotide polymorphisms. All isolates were resistant to penicillin, meropenem, ceftriaxone, and ciprofloxacin but susceptible to vancomycin and linezolid. Most isolates were also resistant to tetracycline, clindamycin, and erythromycin, with susceptibility rates of 10.77, 4.62, and 7.69%, respectively. Genomic analysis revealed 14 antimicrobial resistance genes in the isolates, including tetW, ermX, and sul1. Crystal violet staining showed that all isolates formed biofilms on the abiotic surface. Four clades of multidrug-resistant C. striatum spread in our hospitals possibly due to the acquisition of antimicrobial resistance genes.
Genomic databanks provide robust assessment of invasive mosquito movement pathways and cryptic establishment
Authors: Schmidt Thomas L. , Endersby-Harshman Nancy M. , Kurucz Nina , Pettit William , Krause Vicki L. , Ehlers Gerhard , Muzari Mutizwa Odwell , Currie Bart J. , Hoffmann Ary A. 
Published: June 2023

AbstractBiosecurity strategies that aim to restrict the spread of invasive pests can benefit from knowing where incursions have come from and whether cryptic establishment has taken place. This knowledge can be acquired with genomic databanks, by comparing genetic variation in incursion samples against reference samples. Here we use genomic databanks to characterise incursions of two mosquito species within Australia, and to observe how genomic tracing methods perform when databank samples have limited genetic differentiation and were collected tens of generations ago. We used a deep learning method to trace a 2021 invasion ofAedes aegyptiin Tennant Creek, Northern Territory, to Townsville, Queensland, and to trace two years ofAe. albopictusincursions to two specific islands in the Torres Strait. Tracing had high precision despite 30–70 generations separating incursion and reference samples, and cross-validation of reference samples assigned them to the correct origin in 87% of cases. Similar precision was not achieved with PCAs, which performed particularly poorly for tracing when the invasion had been subject to strong drift effects. Targeted assays also provided additional information on the origin of the Tennant CreekAe. aegypti, in this case by comparingWolbachiainfection data and mitochondrial DNA variation. Patterns of relatedness and inbreeding indicated that Tennant Creek was likely invaded by one family ofAe. aegypti, while Torres Strait incursions were independent and indicated no cryptic establishment. Our results highlight the value of genomic databanks that remain informative over years and for a range of biological conditions.
Saproxylic beetles respond to habitat variables at different spatial scales depending on variable type and species’ mobility: the need for multi-scale forest structure management
Authors: Winiger Nathalie , Hendel Anna-Lena , Ganz Selina , Zielewska-Büttner Katarzyna , Segelbacher Gernot , Braunisch Veronika 
Published: June 2023

AbstractThe response of species to the environment is scale-dependent and the spatial scale at which this relationships are measured may affect conservation recommendations. Saproxylic beetles depend on decaying- and deadwood which occur in lower quantities in managed compared to natural forests. Most studies have investigated the habitat selection of saproxylic beetles at the stand scale, however depending on the species mobility, the amounts and distribution of forest attributes across the landscape may be equally important, and thus crucial to frame quantitative conservation targets. To address this gap, we evaluated the influence of environmental variables, derived from remote sensing across multiple spatial scales (50, 100, 250, 500 and 1000 m radius), on saproxylic beetles habitat selection. Focusing on four mobile and four flightless species, we hypothesized that mobile species respond to habitat variables at broader scales compared to flightless species, and that variables describing forest structure explain species presence better at smaller scales than variables describing other landscape features. Forest structure variables explained around 40% of the habitat selection, followed by variables describing forest type, topography and climate. Contrary to our expectations, mobile species responded to variables at smaller scales than flightless species. Saproxylic beetle species therefore respond to the availability of habitat features at spatial scales that are inversely related to their dispersal capacities, suggesting that less mobile species require larger areas with suitable habitat characteristics while mobile species can also make use of small, distributed patches with locally concentrated habitat features.
KASP mapping of QTLs for yield components using a RIL population in Basmati rice (Oryza sativa L.)
Authors: Ashfaq Hamza , Rani Reena , Perveen Naila , Babar Allah Ditta , Maqsood Umer , Asif Muhammad , Steele Katherine A. , Arif Muhammad 
Published: June 2023

AbstractSuper Basmati is an elite variety with extra-long grains and superior quality but its yield is severely affected by water stress. Recombinant inbred lines (RILs) developed from the cross of Super Basmati and IR55419-04 (a coarse grained variety with high yield potential) were used to identify and map yield-related quantitative trait loci (QTLs) under normal field conditions. Genotypes for 244 KASP markers were obtained from 188 F9 RILs and a linkage map constructed of 1369.4 cM, with average marker interval of 10.5 cM. Eleven agronomic traits were phenotyped in RILs and parents. Composite interval mapping and inclusive composite interval mapping model identified 21 common QTLs related to nine agronomic traits; of the QTLs identified, twelve mapped at novel positions. LOD scores ranged from 2.51 for number of filled grains (qFG1.1) to 16.8 for plant height (qPH1.1). Four major effect QTLs could explain 20% of variation related for plant height, hundred-grain weight and grain width. Alleles from IR55419-04 improved grain filling, grain weight and grain width, while alleles from Super Basmati contributed to longer grains, panicles and flag leaves. Putative candidate genes were identified for 18 yield-related QTLs. These results validate the use of KASP genotyping for QTL mapping of yield-related traits in a bi-parental segregating population. SNPs in the QTLs identified in this study can be used in marker assisted selection for yield components to improve Basmati rice.
Groups of convex bodies
Authors: Hepworth Richard 
Published: June 2023

AbstractIn this paper we introduce and study a topological abelian group of convex bodies, analogous to the scissors congruence group and McMullen’s polytope algebra, with the universal property that continuous valuations on convex bodies correspond to continuous homomorphisms on the group of convex bodies. To study this group, we first obtain a version of McMullen polynomiality for valuations that take values not in fields or vector spaces, but in abelian groups. Using this, we are able to equip the group of convex bodies with a grading that consists of real vector spaces in all positive degrees, mirroring one of the main structural properties of the polytope algebra. It is hoped that this work can serve as the starting point for a K-theoretic interpretation of valuations on convex bodies.
Focused Energy, A New Approach Towards Inertial Fusion Energy
Authors: Ditmire T. , Roth M. , Patel P. K. , Callahan D. , Cheriaux G. , Gibbon P. , Hammond D. , Hannasch A. , Jarrott L. C. , Schaumann G. , Theobald W. , Therrot C. , Turianska O. , Vaisseau X. , Wasser F. , Zähter S. , Zimmer M. , Goldstein W. 
Published: June 2023

AbstractFocused Energy is a new startup company with the goal of developing laser-driven inertial fusion energy for electrical power production. The company combines the results from decades of fundamental research in inertial confinement fusion at universities and national laboratories with the flexibility and the speed of a startup company. Focused Energy has chosen the direct-drive, proton fast ignition approach to reach ignition, burn and high gain as the most promising approach. Located in Austin/US and Darmstadt/Germany, supported by the science community and private investment Focused Energy is paving the way to inertial fusion energy combining the best skill set and state-of-the-art technology from both sides of the Atlantic Ocean. In this paper we discuss the details and reasoning for the approach and the technical directions we have chosen. We will outline our roadmap for getting to a fusion pilot plant in the mid to late 2030s.
Two for the GOES: Exploring Gambling Outcome Expectancies Scores Across Mixed and Offline-Only Gamblers in Relation to Problem Gambling Risk Status
Authors: Richardson Andrea C. , Flack Mal , Caudwell Kim M. 
Published: June 2023

AbstractAs online gambling becomes more prevalent, understanding the motives of online gamblers has become a key focus for research and practice. The aim of this study was to understand differences in gambling-related outcome expectancies between mixed (both online and offline) gamblers and offline-only gamblers, by incorporating gambling harm risk categories from the problem gambling severity index (PGSI). This study comprised a secondary data analysis of the 2015 Northern Territory Gambling Prevalence and Wellbeing Survey. A sample of 1207 individuals in the Northern Territory who had reported gambling at least once in the previous 12 months were used in the analyses. General linear and structural equation modelling were used to ascertain differences in gambling outcome expectancies, in relation to gambling modality (i.e., mixed, offline-only) and PGSI scores. Mixed gamblers tended to score higher on all outcome expectancies than their offline-only counterparts. Outcome expectancy scores were higher in individuals in higher-risk PGSI categories. The escape outcome expectancy was dependent on both modality and risk category. Invariance testing of a low and problem gambling risk subsample revealed differential relationships for both the escape and excitement outcome expectancies for mixed and offline-only gamblers. The results provide an important contribution to the existing literature regarding motivation and outcome expectancies in relation to gambling modality and problem gambling severity. The findings highlight the importance of considering both gambling outcome expectancies and modality when considering problem gambling.
Understanding Clinic and Community Member Experiences with Implementation of Evidence-Based Strategies for HPV Vaccination in Safety-Net Primary Care Settings
Authors: Tsui Jennifer , Shin Michelle , Sloan Kylie , Martinez Bibiana , Palinkas Lawrence A. , Baezconde-Garbanati Lourdes , Cantor Joel C. , Hudson Shawna V. , Crabtree Benjamin F. 
Published: June 2023

AbstractHPV vaccination rates remain below target levels among adolescents in the United States, which is particularly concerning in safety-net populations with persistent disparities in HPV-associated cancer burden. Perspectives on evidence-based strategies (EBS) for HPV vaccination among key implementation participants, internal and external to clinics, can provide a better understanding of why these disparities persist. We conducted virtual interviews and focus groups, guided by the Practice Change Model, with clinic members (providers, clinic leaders, and clinic staff) and community members (advocates, parents, policy-level, and payers) in Los Angeles and New Jersey to understand common and divergent perspectives on and experiences with HPV vaccination in safety-net primary care settings. Fifty-eight interviews and seven focus groups were conducted (n = 65 total). Clinic members (clinic leaders n = 7, providers n = 12, and clinic staff n = 6) revealed conflicting HPV vaccine messaging, lack of shared motivation to reduce missed opportunities and improve workflows, and non-operability between clinic electronic health records and state immunization registries created barriers for implementing effective strategies. Community members (advocates n = 8, policy n = 11, payers n = 8, and parents n = 13) described lack of HPV vaccine prioritization among payers, a reliance on advocates to lead national agenda setting and facilitate local implementation, and opportunities to support and engage schools in HPV vaccine messaging and adolescents in HPV vaccine decision-making. Participants indicated the COVID-19 pandemic complicated prioritization of HPV vaccination but also created opportunities for change. These findings highlight design and selection criteria for identifying and implementing EBS (changing the intervention itself, or practice-level resources versus external motivators) that bring internal and external clinic partners together for targeted approaches that account for local needs in improving HPV vaccine uptake within safety-net settings.
Higher testosterone and testosterone/estradiol ratio in men are associated with decreased Pheno-/GrimAge and DNA-methylation based PAI1
Authors: Kusters Cynthia D. J. , Paul Kimberly C. , Lu Ake T. , Ferruci Luigi , Ritz Beate R. , Binder Alexandra M. , Horvath Steve 
Published: June 2023

Abstract
Sex hormones are hypothesized to drive sex-specific health disparities. Here, we study the association between sex steroid hormones and DNA methylation-based (DNAm) biomarkers of age and mortality risk including Pheno Age Acceleration (AA), Grim AA, and DNAm-based estimators of Plasminogen Activator Inhibitor 1 (PAI1), and leptin concentrations. We pooled data from three population-based cohorts, the Framingham Heart Study Offspring Cohort, the Baltimore Longitudinal Study of Aging, and the InCHIANTI Study, including 1,062 postmenopausal women without hormone therapy and 1,612 men of European descent. Sex-stratified analyses using a linear mixed regression were performed, with a Benjamini-Hochberg (BH) adjustment for multiple testing. Sex Hormone Binding Globulin (SHBG) was associated with a decrease in DNAm PAI1 among men (per 1 standard deviation (SD): -478 pg/mL; 95%CI: -614 to -343; P:1e-11; BH-P: 1e-10), and women (-434 pg/mL; 95%CI: -589 to -279; P:1e-7; BH-P:2e-6). The testosterone/estradiol (TE) ratio was associated with a decrease in Pheno AA (-0.41 years; 95%CI: -0.70 to -0.12; P:0.01; BH-P: 0.04), and DNAm PAI1 (-351 pg/mL; 95%CI: -486 to -217; P:4e-7; BH-P:3e-6) among men. In men, testosterone was associated with a decrease in DNAm PAI1 (-481 pg/mL; 95%CI: -613 to -349; P:2e-12; BH-P:6e-11). SHBG was associated with lower DNAm PAI1 among men and women. Higher testosterone and testosterone/estradiol ratio were associated with lower DNAm PAI and a younger epigenetic age in men. A decrease in DNAm PAI1 is associated with lower mortality and morbidity risk indicating a potential protective effect of testosterone on lifespan and conceivably cardiovascular health via DNAm PAI1.

Graphical Abstract