bluuebunny/crossref_metadata_2025_split huggingface dataset:
Redundant roles of AKT1 and AKT2 in fertility, estrous cyclicity and endometrial gland development
Authors: Adam Pascal , Tardif Laurence , Fabi François , Likonza Lokengo Dadou , Renaud Léa-Isabelle , Parent Sophie , Cadrin Monique , Boerboom Derek , Asselin Eric 
Published: April 2023

In brief
The regulation of AKT in the endometrium during many cellular processes such as apoptosis and cell survival is crucial during the estrous cycle to ensure fertility. This research shows the specific function of AKT isoforms in the mouse endometrium for litter size, estrous cyclicity and endometrial gland development.

Abstract
Apoptosis and cell survival regulation are crucial processes during the estrous cycle to prepare a receptive uterus during implantation for successful recognition of pregnancy. PI3K/AKT signaling has a crucial role during gestation, and AKT isoforms (1, 2 or 3) are regulated differently in the endometrium during the estrous cycle and embryo implantation. However, the specific roles of these isoforms are still unclear. We have previously shown that AKT isoforms expression during the rat estrous cycle and gestation is differently regulated. The present study aimed to establish the specific role of AKT isoforms in the mouse uterus. The hypothesis is that dysregulation of AKT isoforms expression could cause fertility-related issues in an isoform-specific manner. With four different mouse models and in-house crossbreeding, all isoforms KO combinations (single, double and triple) were obtained in progesterone receptor-expressing tissues. The results demonstrated that in absence of one or more AKT isoforms, female fertility was decreased. Mainly, we have observed smaller litter size, specifically in Akt1-2 KO mice. Additionally, we have found Akt1-2-3 KO mice to be fully infertile. Estrous cyclicity was also disrupted in Akt1-2 KO mice with longer diestrus stage. Moreover, the number of endometrial glands was decreased throughout the estrous cycle suggesting an important role in gland development for AKT1 and AKT2. Our results suggest not only specific roles between each isoform but also a partially redundant function of AKT1 and AKT2 in litter size, estrous cyclicity and endometrial gland development. This highlights the importance of AKT in the physiological regulation of mouse fertility.
Intracranial physiological calcifications: A computed tomography study
Authors: Serindere Mehmet , Polat Gokhan 
Published: April 2023

AbstractBackground and aimIntracranial calcifications (ICs) occur when metals such as calcium or iron accumulate in blood vessels, glands or other structures related to the brain. They can be physiological or pathological. Currently, the most sensitive method for imaging IC is cranial computed tomography (CT). The aim of this retrospective study was to evaluate physiological ICs and the co-existence of them by CT.Patients and methods1,000 cranial CT scans of patients were retrospectively evaluated. Pineal calcification, choroid plexus calcification, habenular calcification, petroclinoid ligament calcification, basal ganglia calcification, falx cerebri and tentorium cerebelli calcification were evaluated and recorded.ResultsOf 1,000 patients, 65.7% had IC. The incidence of ICs in different sites were as follows: pineal calcification (37.7%), choroid plexus calcification (52.1%), habenular calcification (33.5%), petroclinoid calcification (21.8%), basal ganglia calcification (0.6%), falx cerebri calcification (6.2%) and tentorium cerebelli calcification (0.2%). The incidence and co-existence of calcifications were significantly higher in females than in males (P < 0.05). Pineal, choroid plexus, petroclinoid ligament calcifications were significantly higher in females than in males (P < 0.05). The incidence and co-existence of calcifications increased with age. Tentorium Cerebelli calcification did not differ significantly between age groups (P > 0.05).ConclusionsICs may be a common finding and encountered incidentally on CT scans which is gold standard to evaluate them. It is important to distinguish physiological calcifications from pathological ones, in the differentiation of pathological lesions with calcifications.
Komodifikasi Filantropi Keagamaan di Harian Umum Republika
Authors: Tabroni Roni , Muhtadi Asep Saeful , Mukarom Zaenal 
Published: April 2023

Kajian ini mengangkat pemberitaan filantropi agama di HU. Republika pada pandemi Covid-19 tahun 2020-2021. Selama dua tahun Republika mengangkat kedermawanan masyarakat Indonesia untuk saling bantu di saat krisis. Berita tersebut dikemas dalam berbagai bentuk dan diletakkan di halaman-halaman yang cukup strategis. Tujuan kajian ini untuk mengungkap pemberitaan filantropi pada masa pandemi Covid-19 serta bagaimana Republika melakukan komodifikasi atas berita tersebut. Kajian ini menggunakan paradigma interpretif dan pendekatakn kualitatif. Sedangkan metode yang digunakan yaitu berupa metode kritis, guna membongkar motif utama di balik pembuatan berita dalam sebuah media. Teori yang digunakan yaitu komodifikasi dan juga turunannya ekonomi politik media. Kajian ini menghasilkan temuan berupa proses komodifikasi yang dilakukan Republika setidaknya pada tiga hal yaitu komodifikasi isi (konten filantropi agama) terhadap pemberitaan filantropi agama selama dua tahun, komodifikasi tenaga kerja (jurnalis) yang sudah dilatih sejak mulai bergabung hingga proses kerjanya, dan komodifikasi audiens (pembaca) yaitu mereka yang terdiri dari komunitas Islam kota sebagai sasarannya. Apa yang dilakukan Republika terhadap konten keagamaan membuktikan terjadinya pergeseran idiologi yang diyakininya selama ini.
HIV self-testing: what GetaKit can tell us about Canada’s $8 million one-time investment
Authors: O’Byrne Patrick , Musten Alexandra 
Published: April 2023

AbstractAt the 16th International AIDS Conference in Montreal, Canada’s Federal Health Minister announced that the Government of Canada will invest $17 million to increase access to HIV testing, $8 million of which would be used to purchase and distribute HIV self-tests. While HIV testing, and subsequent diagnoses, is a critical first step to achieving the updated UNAIDS goals of 95-95-95, testing on its own does not guarantee linkage to treatment or prevention services. In other words, it does not alone guarantee progress toward the 95-95-95 goals. GetaKit, Canada’s first HIV self-test mail-out project, has demonstrated that a preliminary risk-assessment consistent with US CDC and PHAC screening guidelines ensures targeted uptake among communities most affected by HIV, thus minimizing the risk of false positive results and poor positive predictive values. Furthermore, HIV self-testing must link not only individuals with positive results to treatment, but also persons with negative results to pre-exposure prophylaxis (PrEP) along with re-testing as required. However, both access to treatment and PrEP remain inconsistently available across Canada. Therefore, while this one-time investment of funding to increase HIV testing is encouraging, without clear instructions as to who should be prioritized for testing and definitive next steps to ensure that individuals are successfully linked to care, Canada risks wasting resources, further exacerbating pre-existing inequities.
About Some Aspects of Finite State Machine Models Application to Group Control
Authors: Karpov V. E. , Vorobiev V. V. , Rovbo M. A. 
Published: April 2023

The article discusses using discrete-event models to control the coordinated behavior of groups of agents and proposes a mechanism for organizing behavior control based on a hierarchy of finite state machines (FSM). The basic level of agent movement control uses a continuous model of joint movement — the method of potentials, in which the direction of movement is determined by the total sum of the forces of "repulsion", "attraction" and "course alignment". The paper describes a set of independent rules for the group movement of agents — cohesion, distribution, leader following, movement along a chain of landmarks, pursuit, etc. Moreover, all of them are interpreted in terms of the results of the operation of FSM models. Mathematical modeling of controlling a group of agents was carried out using the independent rules of group movement, which confirmed their efficacy. Computational experiments were also carried out using the Gazebo 3D simulator. In addition, the paper describes an experimental group of three underwater vehicles that were used to test the independent rules of group movement: cohesion, distribution, leader following. Communication between the devices was carried out using a system of infrared transceivers via a pseudo-analog channel. The experiments were carried out in a laboratory pool and were successful.The paper shows that the technology for creating group control systems based on the hierarchy of FSM (meta-FSM) allows solving control problems not only at the level of abstract models and simulation modeling, but also at the level of creating real robotic complexes.
INTERNAL FACTOR ATTRIBUTE IN ISLAMIC BANKS: WHICH ONES CONTRIBUTE TO PROFIT DISTRIBUTION? EVIDENCE FROM MUAMALAT BANK
Authors: Iswanto Bambang , Sabna Sauci Fisa’i , Yuliani Irma , Munir Misbahul , Fathah Muhammad Nur Faaiz 
Published: April 2023

Competition between financial institutions will have a positive and negative impact on the development of a bank. In this case, the existence of Bank Muamalat Indonesia as the first pioneer of banks operating with Sharia principles for 30 years needs to be assessed for its financial performance. This study aims to determine and analyze the effect of the ratio of CAR, ROA, FDR, NPF, and BOPO on Profit Distribution Management at Bank Muamalat Indonesia for the 2014-2021 period. The data used in this study includes data on Bank Muamalat Indonesia's quarterly financial statements from 2014 to 2021. The statistical test method used to analyze data in this study is Multiple Regression Analysis. The results of this study show that the ratio of ROA and FDR has a significant effect on Profit Distribution Management, while the ratio of CAR, NPF, and BOPO does not have a significant effect on Profit Distribution Management. Simultaneously, the ratio of CAR, ROA, FDR, NPF, and BOPO affects Profit Distribution Management. This research has several implications for bank policy regarding the importance of maintaining financial performance ratios and opinions through increasing bank financing disbursements and optimizing Profit Distribution Management.
Caractérisation des moutons Peulhs blanc et bicolore du Niger par des variables quantitatives
Authors: Yayé Abdou Hassane , Dayo Guiguigbaza-Kossigan , Abdou Moussa Mahaman Maaouia , Issa Moumouni 
Published: April 2023

Les caractéristiques morphologiques des moutons Peulhs du Niger, à savoir le blanc ou Bali-Bali, et le bicolore ou Oudah, sont très peu connues et peu différenciées. L’objectif de cette étude a été de caractériser quantitativement ces animaux par des mesures morphobiométriques. Ces mesures ont porté sur 27 caractères quantitatifs de 681 individus dont 374 moutons Oudah (312 brebis et 62 béliers) et 307 moutons Bali-Bali (241 brebis et 66 béliers). Les résultats ont montré que les moutons Peulhs étaient de grand format. Les mensurations moyennes des individus, tous adultes, étaient respectivement chez les Bali-Bali et les Oudah de 79,4 ± 4,7 et 80,3 ± 4,6 cm pour la hauteur au garrot, 77,3 ± 5,0 et 77,9 ± 5,4 cm pour la longueur scapulo-ischiale, et 86,7 ± 5,9 et 88,3 ± 6,0 cm pour le tour de la poitrine. Le dimorphisme sexuel était marqué avec des mâles présentant des valeurs moyennes de certains caractères morphologiques significativement supérieures à celles des femelles. Cette étude est une étape dans la contribution à l’élaboration de stratégies durables de gestion, de conservation et d’amélioration des moutons Peulhs du Niger.
Extended Neoadjuvant Chemotherapy in Delayed Primary Resection of Ewing Sarcoma During the COVID-19 Era
Authors: Arunwatthanangkul Patcharapol , Rangchaikul Nutnicha , Anuwongworavet Supitcha , Chobpenthai Thanapon 
Published: April 2023

Case:
A 13-year-old adolescent boy visited our hospital with a growing mass on his left leg. Investigations and examinations were performed to obtain a final diagnosis of Ewing sarcoma in the head of the left fibula with lung metastasis. Neoadjuvant chemotherapy was extended to 11 courses with radiation before wide tumor resection could be performed. The final 3 adjuvant chemotherapy courses were administered to complete the original protocol while surgical resection complications were also treated. The pathological report revealed free margin resection with nonviable tumor cells.

Conclusion:
An extended neoadjuvant chemotherapy regimen with additional radiation therapy for Ewing sarcoma provided extra local control and allowed limb salvage.
Surgical Management of Hip Dislocation in a Patient with SCARF Syndrome
Authors: Chandankere Vidyasagar , Reddy Maryada Venkateshwar , A.V. Reddy Gurava 
Published: April 2023

Case:
We report a six-year-old child with SCARF syndrome (skeletal anomaly, cutis laxa, ambiguous genitalia, mental retardation and distinct facial features) who presented with unilateral teratologic hip dislocation. She underwent an open reduction of her hip with femoral and pelvis osteotomies. At six years follow-up, she was asymptomatic with a mild lurch, a leg length discrepancy of 1.5cms and a good range of motion at the hip. A mild shortening of the femoral neck was noted but the joint was congruous and concentrically reduced at 6 years.

Conclusion:
The management principles must follow an aggressive approach which includes open reduction of the hip, femoral and pelvic osteotomies with a good capsular repair. We may expect good hip development after surgical intervention even in a child with increased elasticity due to this genetic condition.
Rapid screening methods for universal binding peptide aptamers against SARS-CoV-2 variant spikes, including omicron variants, and their application to diagnostic and therapeutic agents.
Authors: Hayashi Nakanobu , Abe Chikako , Kikuchi Jiro , Hayashi Momoko , Hayashi Sakura , Ueda Masahiro , Suzuki Koyu , Sugitani Masahiko , Taniguchi Hiroaki , Wake Toru , Furukawa Yusuke 
Published: April 2023

Abstract
The development of mRNA vaccines and oral drugs against SARS-CoV-2 has been useful in protecting against Covid-19 infection. Since then, however, many variants of delta and omicron strains with enhanced infectivity and immune escape capacity have emerged. A 7-amino acid random peptide ribosome display library screening system was used to perform a rapid in vitro screening of peptide aptamers that universally bind to the SARS-CoV-2 wild-type, delta, and Omicron variant BA.1, BA.2, and BA.5 spike RBD (Receptor Binding Domain). Screening resulted in four peptide aptamers that showed positive binding reactions in ELISA. Interestingly, Amino Acid Sequence Determination of the four clones predicted that three of the four clones contain 2 ~ 3 Cys residues in their sequences, forming a complex higher-order structure with disulfide (S-S) bonds. The 7-amino acid random peptide ribosome display library screening system allows for rapid in vitro screening of peptide aptamers that bind to other unknown emerging infectious disease pathogens that may be pandemic in the future. The peptide aptamers are as small as 30 amino acids and can be easily synthesized and purified as peptides or proteins, or simply used as mRNA drugs.
Preoperative Supine Time for Adrenal Venous Sampling: A Prospective Randomized Controlled Trial
Authors: Liu Zhenjie , He Minzhi , Zhang Yuhao , Song Xiaoxiao , Zhang Tianyue , Yu Hailan , Chai Peifei , Chen Bing , Xu Xiaohong 
Published: April 2023

Abstract
Background: Primary aldosteronism (PA) is the most common form of secondary hypertension, accounting for 5% of hypertensive patients and 17-23% in patients with resistant hypertension. Compared to the primary hypertension, PA is more prone to cause severe organ damage and even early death. Adrenal venous sampling (AVS) is an effective confirmatory test for subtyping unilateral and bilateral adrenal hyperplasia, helping physicians to make an accurate decision between surgery or medication to treat PA. Supine in bed before AVS is recommended for a desirable result of AVS according to guidelines. However, investigating study about the most optimal preoperative supine time before AVS is lacking.
Methods/design: This is a single-center prospective randomized controlled study. 120 patients diagnosed as PA and with willing for further AVS examination will be included. Participants will be randomly allocated to 15-min supine time group or 2-hours supine time group. The primary outcome is the degree of biochemical remission (serum potassium and orthostatic ARR). The secondary outcomes are degree of the clinical remission (blood pressure, type and dose of antihypertensive drugs), the technical success rate and the adverse event of AVS (selective index≥2 is considered as successful surgery without corticotropin stimulate).
Discussion: PA is an intractable public health problem, and many techniques including AVS have been developed to correctly identify this disease. This study will help to understand whether the length of preoperative supine time would affect the diagnostic efficacy of AVS, and thus help to formulate a more reasonable AVS procedure.
Trial registration: ClinicalTrials.gov, ID: NCT05658705. Registered on 10 September 2022.
Associations of Antihypertensive Medication Consumption and Drug-Drug Interaction with Statin and Metformin with Reduced Alzheimer's Disease and Related Dementias Risk among Hypertensive Patients with Mild Cognitive Impairment using High Volume Claims Data
Authors: Tao Cui , Lundin Sori , Hu Xinyue , Feng Jingna , Lundin Karl , Chen Yong 
Published: April 2023

Abstract
Background: While hypertension is a modifiable risk factor of Alzheimer's disease and related dementias
(ADRD), limited studies have been conducted on the effectiveness of antihypertensive medications (AHMs) in altering the progression from mild cognitive impairment (MCI) to ADRD; similarly, few studies have assessed drug-drug interactions of AHMs with drugs targeted to modify other risk factors of ADRD such as type Ⅱ diabetes and hypercholesterolemia.
Method: 128,683 unique hypertensive patients with MCI on US-based Optum claims data were identified. Diuretics, beta blockers (BBs), calcium channel blockers (CCBs), angiotensin-converting enzyme inhibitors (ACE inhibitors), and angiotensin II receptor antagonists (ARBs) were identified as five major AHM classes. Baseline characteristics were compared. Cox proportional hazards (PH) models were used to study the association between specific AHM exposure and the progression from MCI to ADRD while controlling for demographic variables, comorbidities, and the use of Statins and Metformin. To examine the association of AHM-Statin or AHM-Metformin interaction with ADRD progression, we also investigated models controlling for the aforementioned confounders, as well as drug-drug interactions.
Result: The study included 100,678 patients who were taking at least one class of AHM and 28,005 who were not taking any AHMs during the study period. AHM users had a higher incidence of comorbidities (all P≤0.039) and consumption of Metformin and Statins (both P<0.001) compared to non-users. Users of each major AHM class showed significantly lower risk of developing ADRD compared to non-users of that specific drug class (adjusted hazard ratio (aHR): 0.96-0.98; all P≤0.048). Within patients on monotherapy (using only one AHM drug), no specific AHM class had significantly lower risk of ADRD diagnosis compared to other AHM drug classes (aHR: 0.97-1.11; all P≥0.053). Use of Diuretics or CCBs in combination with Metformin consumption (aHR: 0.89, 0.91, respectively) showed lower risk of MCI to ADRD progression than use without Metformin consumption (aHR: 0.97, 0.98, respectively), whereas use of any of the five major AHMs with Statin consumption (aHR: 0.91-0.94) all showed lower risk than without Statin consumption (aHR: 0.98-1.04).
Conclusion: All five major AHM classes showed a protective effect against ADRD progression among hypertensive patients with MCI. Also, certain combinations of AHMs with Metformin or Statins showed a stronger protective effect compared to AHMs alone, and some drug-drug interactions of AHMMetformin or AHM-Statin also showed protective effects against progression from MCI to ADRD.
Heterogenous biosynthesis of medicarpin using engineered Saccharomyces cerevisiae
Authors: Lu Chujie , Du Rui , Fu Hao , Zhang Jizhao , Zhao Ming , Wei Yongjun , Lin Wei 
Published: April 2023

Abstract
Background
Medicarpin is one important bioactive compound with multiple medicinal activities, including anti-tumor, anti-osteoporosis, anti-bacterial effects. Medicarpin is assigned to pterocarpans derived from medicinal plants, such as Sophora japonica, Glycyrrhiza uralensisFisch. and Glycyrrhiza glabra L.However, these medicinal plants only contain low amounts of medicarpin. Moreover, the planting area for medicarpin-producing plants is limited, thus, current medicarpin supply cannot satisfy the great demands of medicinal markets.
Results
In this study, eight key genes involved in medicarpin biosynthesis were identified by comparative transcriptome analysis and bioinformatic analyses. In vitro and in vivoenzymatic activities assays confirmed the catalytic functions of candidate enzymes were responsible for the biosynthesis of medicarpin and medicarpin intermediates. Further engineering of these genes in Saccharomyces cerevisiae achieved the heterogenous biosynthesis of medicarpin using liquiritigenin as the substrate, and the final medicarpin titer was 0.82 ± 0.18 mg/L. By increasing the gene copy number of VRand PTS, the final titer of the medicarpin increased to 2.05 ± 0.72 mg/L.
Conclusion
This study provides a solid foundation for the economical and sustainable production of medicarpin by synthetic biology strategy.
Integration of phenylalanine precursor feeding and elicitor treatment in concert with the cellulase-assisted extraction approach to improve stilbene compound production
Authors: Tothong Chonnikan , Pankaew Chanyanut , Chayjarung Phadtraphorn , Kongbangkerd Anupan , Limmongkon Apinun 
Published: April 2023

Abstract
Trans-resveratrol (RES), trans-arachidin-1 (Ara-1), and trans-arachidin-3 (Ara-3) are major stilbene compounds found in elicited peanut hairy root culture and exert diverse potential biological and pharmaceutical activities. The aim of this study was to investigate the various approaches for enhancing the productivity of prenylated stilbene. The fast-growing peanut hairy root K2-K599-C line was elicited with chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD) (CHT+MeJA+CD) as combined elicitors together with phenylalanine (Phe) as a precursor to feeding at 24, 48, and 72 h. The accumulation of stilbene compounds was investigated in this study as well as stilbene biosynthesis-related genes and plant defense gene expression. Upon an exogenous supply of Phe, elevated amounts of Ara-1 and Ara-3 were detected through the entire 72 h elicitation period while the non-Phe precursor feeding conditions exhibited an increasing amount of Ara-1 and Ara-3 up to 48 h, decreasing at 72 h. In addition, Phe precursor may serve as an early regulator of gene expression for the stilbene biosynthesis pathway, transcription factors, and pathogenesis-related proteins while a delayed response in transcriptional regulation was observed in the elicitation without Phe precursor feeding. Cellulase-assisted extraction was performed prior to solvent extraction of the culture medium. The results indicated a significant 1.73-fold and 1.57-fold increase in Ara-1 and Ara-3, respectively, compared to the non-cellulase-assisted process. To the best of our knowledge, this is the first study to propose an integrated approach to improving stilbene compound production in peanut hairy root culture.