bluuebunny/crossref_metadata_2025_split huggingface dataset:
Daily fluctuations in spinal adenosine determine mechanisms of respiratory motor plasticity
Authors: Marciante Alexandria B. , Seven Yasin B. , Kelly Mia N. , Perim Raphael R. , Mitchell Gordon S. 
Published: December 2022

ABSTRACTPlasticity is a fundamental property of the neuromotor system controlling breathing. One key example of respiratory motor plasticity is phrenic long-term facilitation (pLTF), a persistent increase in phrenic nerve activity after exposure to intermittent low oxygen or acute intermittent hypoxia (AIH). pLTF can arise from distinct intracellular signaling cascades initiated by serotonin and adenosine; these cascades interact via powerful crosstalk inhibition. We demonstrate the serotonin/adenosine balance varies dramatically with time-of-day and details of the AIH protocol. Using a “standard” AIH protocol, the mechanism driving pLTF shifts from serotonin-dominant, adenosine-constrained during rest, to adenosine-dominant, serotonin-constrained in the active phase. This mechanistic ‘flip’ results from daily changes in basal spinal adenosine levels across time-of-day combined with hypoxia-evoked spinal adenosine release. Since AIH is emerging as a promising therapeutic modality to restore respiratory (and non-respiratory) movements in people with spinal injury or ALS, new knowledge that time-of-day and protocol details impact mechanisms driving pLTF has experimental, biological and translational implications.
Mechanical regulation of retinal vascular inflammation and degeneration in diabetic retinopathy
Authors: Chandrakumar Sathishkumar , Tierno Irene Santiago , Agarwal Mahesh , Lessieur Emma M. , Du Yunpeng , Tang Jie , Kiser Jianying , Yang Xiao , Kern Timothy S. , Ghosh Kaustabh 
Published: December 2022

ABSTRACTVascular inflammation is known to cause degeneration of retinal vessels in early diabetic retinopathy (DR). Past studies investigating these diabetes-induced vascular abnormalities have focused primarily on the role of molecular or biochemical cues. Here we show that retinal vascular inflammation and degeneration in DR are also mechanically regulated by retinal vascular stiffening that is caused by overexpression of collagen-crosslinking enzyme lysyl oxidase (LOX) in retinal vessels. Treatment of diabetic mice with LOX inhibitor BAPN prevented the increase in retinal vascular stiffness, vascular ICAM-1 overexpression, and leukostasis. Consistent with these anti-inflammatory effects, BAPN treatment of diabetic mice blocked the upregulation of proapoptotic caspase-3 in retinal vessels, which concomitantly reduced retinal vascular degeneration and the diabetes-induced loss of contrast sensitivity in these mice. Finally, we show that increasing substrate stiffness alone increases the adhesiveness and neutrophil elastase-induced death of cultured retinal endothelial cells. By uncovering a link between LOX-dependent vascular stiffening and the development of retinal vascular and functional defects in diabetes, these findings offer unique insights into DR pathogenesis that has important translational potential.
A multi-method study to identify and validate alcohol diagnostics for injury-related trauma in South Africa: a study protocol
Authors: Williams Petal Petersen , Prinsloo Megan R. , Peden Margaret M. , Neethling Ian , Mhlongo Shibe , Maqungo Sithombo , Parry Charles , Matzopoulos Richard 
Published: December 2022

ABSTRACTIntroductionThe burden of alcohol use among trauma patients and the relative injury risks is not routinely measured in South Africa (SA). Given the prominent burden of alcohol on hospital trauma departments, SA needs practical, cost-effective and accurate alcohol diagnostic tools for testing, surveillance and clinical management of trauma patients. This study aims to validate alcohol diagnostics for injury-related trauma and assess its utility for improving national health practice and policy.Methods and AnalysisThe Alcohol Diagnostic Validation for Injury-Related Trauma (AVIRT) study will use mixed methods across three work packages. Focus Group Discussions will be conducted with key stakeholders across four areas of expertise (clinical, academic, policy and operational) to determine the type of alcohol information that will be useful for different stakeholders in the injury prevention and healthcare sectors. We will then conduct a validation study of alcohol diagnostic tools (clinical assessment, breath analysis, finger-prick blood) against enzyme immunoassay blood concentration analysis in a tertiary hospital trauma setting. Finally, selected alcohol diagnostic tools will be tested in a district hospital setting alongside community-based participatory research on the utility of the selected tools.Ethics and disseminationThe study was approved by the Research Ethics Committee of the South African Medical Research Council and the Western Cape Health Department. Findings will be disseminated to inform strategies to introduce routine, cost-effective alcohol diagnostics optimally in a high trauma setting by ensuring accuracy, real world feasibility and institutional support.Strengths and limitations of this studyThe study will provide an understanding of the impact of alcohol and its association with injuries, highlight the burden alcohol-related injuries impose on health workers, inform policies to mitigate alcohol harm, and recommend methods to scale-up alcohol screening and detection in trauma patients nationally.The study setting will complement the limited evidence base on alcohol consumption in LMICs.Expert stakeholders included in focus group discussions will be restricted to known contacts within the field.Validity testing of the alcohol diagnostics will use enzyme immune assay instead of the gold standard gas chromatography method, which was too expensive.Results will be generalisable to patients treated at public-sector district and tertiary-level facilities, that treat a higher proportion of moderate to severe injuries.
A bispecific antibody NXT007 exerts a hemostatic activity in hemophilia A monkeys enough to keep a non-hemophiliac state
Authors: Teranishi-Ikawa Yuri , Soeda Tetsuhiro , Koga Hikaru , Yamaguchi Kazuki , Kato Kazuki , Esaki Keiko , Asanuma Kentaro , Funaki Miho , Ichiki Mina , Ikuta Yuri , Ito Shunsuke , Joyashiki Eri , Komatsu Shun-Ichiro , Muto Atsushi , Nishimura Kei , Okuda Momoko , Sanada Hisakazu , Sato Motohiko , Shibahara Norihito , Wakabayashi Tetsuya , Yamaguchi Koji , Matsusaki Akiko , Sampei Zenjiro , Shiraiwa Hirotake , Konishi Hiroko , Kawabe Yoshiki , Hattori Kunihiro , Kitazawa Takehisa , Igawa Tomoyuki 
Published: December 2022

AbstractEmicizumab, a factor (F)VIIIa-function mimetic bispecific antibody (BsAb) to FIXa and FX, has become an indispensable treatment for people with hemophilia A (PwHA). Although emicizumab is very potent, long-term outcomes from the clinical studies suggest that a small proportion of PwHA still experiences bleeds. Additionally, non-clinical studies indicate that the maximum cofactor activity of emicizumab is lower than international standard activity (100 IU/dL of FVIII). An increased cofactor activity BsAb would benefit such patients. Here, we report NXT007, a BsAb binding FIXa and FX developed through further engineering of emicizumab. Emicizumab has a common light chain, but through advances in antibody engineering, we were able to create a more potent BsAb with two new non-common light chains. After extensive optimization of the heavy and light chains, the resulting BsAb, NXT007, exerted in vitro thrombin generation (TG) activity in hemophilia A plasma equivalent to 100 IU/dL of FVIII when triggered by tissue factor. NXT007 demonstrated potent hemostatic activity in an acquired hemophilia A model in non-human primates at a much lower dosage than emicizumab, consistent with an around 30-fold dose shift in the in vitro TG activity between NXT007 and emicizumab. Moreover, together with Fc engineering that enhanced FcRn binding and reduced in vivo clearance, we demonstrate that NXT007 could be effective at a much lower dosage with a longer dosing interval compared to emicizumab. These non-clinical results suggest that NXT007 could maintain a non-hemophilic range of coagulation potential in PwHA and provides a rationale for its clinical testing.
Inverse identification of region-specific hyperelastic material parameters for human brain tissue
Authors: Hinrichsen Jan , Reiter Nina , Bräuer Lars , Paulsen Friedrich , Kaessmair Stefan , Budday Silvia 
Published: December 2022

The identification of material parameters accurately describing the region-dependent mechanical behavior of human brain tissue is crucial for computational models used to assist, e.g., the development of safety equipment like helmets or the planning and execution of brain surgery. While the division of the human brain into different anatomical regions is well established, knowledge about regions with distinct mechanical properties remains limited. Here, we establish an inverse parameter identification scheme using a hyperelastic Ogden model and experimental data from multi-modal testing of tissue from 19 anatomical human brain regions to identify mechanically distinct regions and provide the corresponding material parameters. We assign the 19 anatomical regions to nine governing regions based on similar parameters and microstructures. Statistical analyses confirm differences between the regions and indicate that at least the corpus callosum and the corona radiata should be assigned different material parameters in computational models of the human brain. We provide a total of four parameter sets based on the two initial Poisson’s ratios of 0.45 and 0.49 as well as the pre- and unconditioned experimental responses, respectively. Our results highlight the close interrelation between the Poisson’s ratio and the remaining model parameters.The identified parameters will contribute to more precise computational models enabling spatially resolved predictions of the stress and strain states in human brains under complex mechanical loading conditions.
LRRK2 Kinase Activity Regulates Parkinson’s Disease-Relevant Lipids at the Lysosome
Authors: Maloney Michael T. , Wang Xiang , Ghosh Rajarshi , Andrews Shan V. , Maciuca Romeo , Masoud Shababa T. , Caprioli Richard M. , Chen John , Chiu Chi-Lu , Davis Sonnet S. , Ho Audrey Cheuk-Nga , Nguyen Hoang N. , Propson Nicholas E. , Reyzer Michelle L. , Davis Oliver B. , Deen Matthew C. , Zhu Sha , Di Paolo Gilbert , Vocadlo David J. , Estrada Anthony A. , de Vicente Javier , Lewcock Joseph W. , Arguello Annie , Suh Jung H. , Huntwork-Rodriguez Sarah , Henry Anastasia G. 
Published: December 2022

AbstractLysosomal dysfunction is a hallmark of Parkinson’s disease (PD), and variants in several PD-associated genes, includingLRRK2, perturb lysosomal homeostasis. Based on this, LRRK2 kinase inhibition is being explored as a therapeutic approach for the treatment of PD. LRRK2 inhibitors reduce levels of BMP, an endolysosomal lipid involved in glycosphingolipid (GSL) catabolism, in urine from preclinical models and clinical subjects, however, the mechanisms by which LRRK2 regulates BMP and the functional significance of this change to disease are undefined. We establish that LRRK2 regulates secretion of BMP- and GSL-containing vesicles from kidney into urine and modulates BMP and GSL levels in the brain. BMP accumulates within lysosomes as a secondary response to LRRK2’s effects on the activity of glucocerebrosidase (GCase), a PD-linked enzyme involved in GSL catabolism. Alterations in BMP and GCase substrate turnover are observed in CSF from LRRK2-PD patients, highlighting the relevance of LRRK2-dependent lysosomal dysfunction in disease.
Silencing neuroinflammatory reactive astrocyte activating factors ameliorates disease outcomes in perinatal white matter injury
Authors: Renz Patricia , Surbek Daniel , Haesler Valérie , Tscherrig Vera , Huang Eric J , Chavali Manideep , Liddelow Shane , Rowitch David , Schoeberlein Andreina , Lutz Amanda Brosius 
Published: December 2022

AbstractThe role of reactive astrocytes in perinatal white matter injury (WMI) is unclear. In a mouse model of WMI, we provide evidence that impairing the formation of aC3-expressing neuroinflammatory reactive astrocyte sub-state rescues myelination and behavioral deficits. We further demonstrate the presence ofC3-expressing reactive astrocytes in human WMI. Our data point to these cells as putative drivers of myelination failure in WMI and a potentially promising therapeutic target.
Investigating forms of graduate capital and their relationship to perceived employability: an application of PLS predict and IPMA
Authors: Agnihotri Shikha , Shiva Atul , Khan Farha Naz 
Published: December 2022

PurposeThe study aims to assess the relationship between cultural capital, human capital, psychological capital, social capital and perceived employability of management graduates.Design/methodology/approachThe data was analysed through variance based partial least square (PLS) structural equation modelling on 505 management students by an online questionnaire. The predictive relevance of perceived employability was investigated with PLS predict approach. Further, importance-performance map analysis (IPMA) was applied to assess important and performing dimensions of perceived employability.FindingsThe results indicate that social capital was found to be the strongest predictor of graduates' perceived employability. The proposed conceptual model was found to have a moderate to high predictive relevance. IPMA results suggested that investment in psychological capital leads to higher return in perceived employability of management graduates.Research limitations/implicationsData was collected using purposive sampling and confined to university students only.Practical implicationsFindings of the study provide empirical inferences in support of human capital, social capital and social cognitive theory. Practical implications offer important inputs to policy makers, higher educational institutes, career counsellors and universities.Originality/valueThis study provides novel inputs by a comprehensive model of graduate capital to determine and predict perceived employability of graduates in emerging economy like India.
Computational study of dynamics of confined droplets under electric field: effect of contact angle
Authors: Cao Qianqian , Li Lujuan , You Hao , Liu Hao 
Published: December 2022

Purpose
The contact behaviors of droplets on confined surfaces influence significantly their dynamics and morphological transition induced by the electric field. This paper aims to delve into the electric stress, electric field distribution, flow field and evolution of droplet neck to understand the underlying mechanisms.

Design/methodology/approach
Electrohydrodynamics of droplets in confined environment is numerically analyzed based on finite volume method (FVM) combining with volume-of-fluid (VOF) method for two-phase interface capturing. Numerical solutions are obtained through solving electrohydrodynamics model coupling fluid dynamics with electrostatics.

Findings
It was found that the droplet neck with high interfacial curvature undergoes different transition depending on the contact angle. At large domain height, the droplets on the surfaces with the contact angle of θ < 90° tend to break up into smaller droplets adhered on top and bottom surfaces. The detachment of droplets is identified when the contact angle is much greater than 90°. Notably, the droplets at θ = 90° exhibit asymmetrical shape evolution, but for other cases there is symmetrical shape of droplets during transition process. With decreasing the domain height, no obvious deformation through driving the contraction of the droplet neck is observed.

Originality/value
It remains unclear how the electric field parallel to the surfaces affects the shape transition and electrohydrodynamics of confined droplets when changing the contact angle. In this paper, the authors study the electrohydrodynamics of droplets in confined space when the electric field is exerted parallel to contact surfaces. In particular, the authors consider the effect of the surface wettability on the droplet deformation. The problem is solved through FVM combining with the VOF method to implement the capturing of two-phase interfaces. The results indicate that the electrohydrodynamic behaviors of droplets are sensitive to the contact properties of droplets on the surfaces, which has not been reported in previous works.
Toward the development of an Islamic banking sustainability performance index
Authors: Ammar Rym , Rebai Sonia , Saidane Dhafer 
Published: December 2022

Purpose
The purpose of this paper is to suggest a model that yields a sustainability performance index for Islamic banks (IBs). This index is expected to account for stakeholders’ viewpoints while considering sustainability and Maqasid Al-Shariah as bases.

Design/methodology/approach
First, based on the relevant literature review refined through consultations with academic, banking and Shariah experts, the main stakeholders and their corresponding lists of relevant attributes and sub-attributes are identified. Then, adopting a multi-attribute utility approach and based on a second step of interviews with experts, an aggregated index is suggested. Finally, the developed index is applied to five famous Islamic banking groups over the period 2005–2019.

Findings
Empirical evidence shows that the banks used in the implementation do not achieve high scores of the suggested index. This can be interpreted through a lack of Islamic normative aspects and low adherence to sustainability practices. Specifically, they are not functioning on a justice basis and are deficient in providing sufficient varieties of Islamic products. They are also more interested in economic sustainability and are not involved in environmental and social ones.

Originality/value
The developed index not only considers the compliance of the banking activities with Shariah, but it also addresses their sustainability from the main stakeholders’ perspectives. The suggested model provides a transparent performance evaluation tool for IBs omitting all causes of conflict of interests and certifies the fairness of the resulting assessments.
Event-triggered aperiodic intermittent sliding-mode control for master–slave bilateral teleoperation robotic systems
Authors: Gao Hang , Ma Chao 
Published: December 2022

Purpose
The purpose of this paper is to propose a novel event-triggered aperiodic intermittent sliding-mode control (ETAI-SMC) algorithm for master–slave bilateral teleoperation robotic systems to further save communication resources while maintaining synchronization precision.

Design/methodology/approach
By using the Lyapunov theory, a new event-triggered aperiodic intermittent sliding-mode controller is designed to synchronize master–slave robots in a discontinuous method. Unlike traditional periodic time-triggered continuous control strategy, a new ETAI condition is discussed for less communication pressure. Then, the exponential reaching law is adopted to accelerate sliding-mode variables convergence, which has a significant effect on synchronization performance. In addition, the authors use quantizers to make their algorithm have obvious progress in saving communication resources.

Findings
The proposed control algorithm performance is validated by an experiment developed on a practical bilateral teleoperation system with two PHANToM Omni robotic devices. As a result, the synchronization error is limited within a small range and the control frequency is evidently reduced. Compared with a conventional control algorithm, the experimental results illustrate that the proposed control algorithm is more sensitive to system states changes and it can further save communication resources while guaranteeing the system synchronization accuracy, which is more practical for real bilateral teleoperation robotic systems.

Originality/value
A novel ETAI-SMC for bilateral teleoperation robotic systems is proposed to find a balance between reducing the control frequency and synchronization control precision. Combining the traditional sliding-mode control algorithm with the periodic intermittent control strategy and the event-triggered control strategy has produced obvious effect on our control performance. The proposed ETAI-SMC algorithm helps the controller be more sensitive to system states changes, which makes it possible to achieve precise control with lower control frequency. Moreover, we design an environment contact force feedback algorithm for operators to improve the perception of the slave robot working environment. In addition, quantizers and the exponential convergence law are adopted to help the proposed algorithm perform better in saving communication resources and improving synchronization precision.
Can Alexa serve customers better? AI-driven voice assistant service interactions
Authors: Malodia Suresh , Ferraris Alberto , Sakashita Mototaka , Dhir Amandeep , Gavurova Beata 
Published: December 2022

Purpose
This study aims to examine customers’ willingness to engage in service interactions enabled by artificial intelligence (AI) controlled voice assistants (VA). Drawing on the tenets of dual-factor theory, this study measures the impact of both enablers and inhibitors – mediated by trust in Alexa – on customers’ intentions to transact through VAs.

Design/methodology/approach
Data from a survey of 290 users of VAs from Japan was collected through “Macromill”. The authors used a covariance-based path analysis technique for data analysis after establishing the validity and reliability of the measures.

Findings
The results of this study demonstrate that convenience and status-seeking act as enablers and positively influence trust in VAs, whereas risk barrier acts as an inhibitor and negatively influence trust in VAs. In turn, trust in VAs positively influences the intention to use VAs for transactional service interactions. This association is positively moderated by technology comfort.

Originality/value
This study applies dual-factor theory to the context of VAs – a context that scholars have, to date, examined solely from a technology adoption perspective. For the first time, the authors adopt a dual-factor approach to identify a new set of antecedents for customers’ intentions to use VAs for transactional service interactions.
Audit partner characteristics, career advancement and audit quality in the USA
Authors: Cai Chen , Ciccone Stephen , Li Huimin , Xu Le Emily 
Published: December 2022

Purpose
This study aims to explore the relation among US audit partners’ characteristics, their career advancement and audit quality.

Design/methodology/approach
This study uses data from Public Company Accounting Oversight Board Form AP, Auditor Reporting of Certain Audit Participants, and publicly available online data sources. The hand-collected data on audit partners’ personal characteristics include gender, work experience and educational background. The measures for audit quality include restatements and audit fees.

Findings
The authors find that audit partner characteristics matter for the time it takes an individual to reach partnership after completing a bachelor’s degree. There are significant differences in work experience and educational background between partners in the largest (Big N) audit firms and smaller (non-Big N) audit firms. Audit partner traits are related to audit quality, and the effects differ between Big N and non-Big N partners.

Originality/value
The literature has examined audit partners’ career paths using international data. However, little empirical academic research has examined the career advancement of US audit partners. This study provides initial insights on the career advancement of US partners on a large scale and complements the recent research that examines audit partner characteristics and audit quality in the US market.