bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using immunoinformatic and reverse vaccinology approach epitope-based Vaccine design for three Vibrio sp
Authors: Baradia Hrithik , Mukherjee Koel 
Published: April 2023

Abstract
Aquaculture is a rapidly growing industry both in India and globally. After Carps, Tilapia is the most cultured fish in the industry. Due to stress and overexploitation of the aquatic ecosystem, there has been rise and propagation of variety of diseases which is a major bottleneck for the industry. Bacterial diseases particularly Vibriosis caused by V. vulnificus, V. harveyi and V. alginolyticus is of great concern as it causes severe illness in humans along with the aquatic organisms. Using reverse vaccinology technique, arranging the epitopes present in OMPs of V. vulnificus, V. harveyi and V. alginolyticus with respect to MHC-I, MHC-II, T-cell and B-cell with the help of advanced bio-computational techniques, it is predicted that YTSTDNFAV, QSIQGNTMN and SQLCIAISL might be potential vaccine candidates elucidating both humoral and cell-mediated immune response. A multi-epitope vaccine is also designed to target the three microbes together by linking the peptide sequences using amino acid linker GPGPG. The cholera toxin B subunit was added in the adjuvant in the N-terminal side using EAAAK linker. Analysis of the multi-epitope vaccine candidate showed that it has all the properties of a potential robust vaccine including antigenic, immunogenic, soluble and thermostable.
Machining deformation control methods and analysis of a thin-wall gear spoke plate
Authors: Huan Haixiang , Zhang Ke , Xu JiuHua , Xu WenQiang , Wang MengXiong 
Published: April 2023

Abstract
This paper addresses the problems of easy deformation, difficult dimensional control, and difficult accuracy assurance during the production of thin-walled gears for helicopter spoke cutting, It uses finite element simulation technology, metal cutting principles, process optimization and other design and manufacturing technologies to reveal the causes of cutting deformation, effectively predict the deformation of parts during cutting, and develop reasonable deformation control strategies. This study explores the effects of cutting force, residual stress and clamping method on gear machining deformation. The deformation of the spoke plate is analysed by finite element simulation. Abaqus software,is adopted to establish a finite element model for 2-D turning simulation to predict the machining residual stresses and cutting forces during gear turning machining with different cutting parameters. The simulated cutting forces are compared with the residual stresses and experimentally measured values to verify the accuracy of the finite element model and provide a theoretical basis for model construction in the next simulation study. With Abaqus software, the effect of different cutting forces on the deformation of the spoke plate is simulated, and the effect of the superposition of cutting and clamping forces on the deformation of the spoke plate is simulated using life and death cell technology to simulate the machining of the workpiece. Then, a corresponding fixture is designed to realise the corresponding clamping position for machining experiments, and the deformation of the spoke plate is measured by CMM(Coordinate Measuring Machine) to verify the realism of the simulation. This research provides scientific theoretical guidance and process support for aerospace thin-walled gear spoke plate turning.
Characteristics of pPLA family and their roles in reproductive development of Gossypium hirsutum L
Authors: Wang Mingyang , Tian Dingyan , Li Tengyu , Pan Jingwen , Wang Chenlei , Wu Lanxin , Luo Kun , Mei Zhenyu , Liu Jinwei , Chen Wei , Yao Jinbo , Li Yan , Wang Fuxin , Zhu Shouhong , Zhang Yongshan 
Published: April 2023

Abstract
Background Phospholipases As (PLAs) are acyl hydrolase that can catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. PLAs can be divided into three families, PLA1, PLA2 (sPLA) and patatin-related PLA (pPLA). Previous studies have elucidated the versatile roles of pPLAs in growth and development of many plants.Methods Genome-wide analysis of the pPLA family and screening of genes for expression verification and gene silencing verification. After gene silencing, analysis of pollen expression pattern, pollen vitality test and POD, SOD, CAT, MDA and H2O2 detection.Result In this study, a total of 294 pPLAs were identified from 13 species, including 46 GhpPLAs divided into three subfamilies (I-III-γ). Expression pattern analysis revealed that most of GhpPLAs were preferentially expressed in the reproductive organs (petal, pistil, anther and ovule), especially two genes (GhpPLA23 and GhpPLA44) were deduced that may affect the reproductive development of G. hirsutum. VIGS experiment of GhpPLA23 and GhpPLA44 showed that the pollen activity decreased with their lower expression levels in the silenced plants. The contents of POD, CAT, SOD and other physiological indicators were significantly increased, while ROS and ROS scavengers were significantly changed after genes silencing, indicating that these two genes had important effects on cotton reproductive development. These findings suggest that pPLAs play a crucial role in reproductive development of G. hirsutum and show the potential to be utilized as candidate genes for haploid inducing.Conclusions In this study, pPLA genes play an important role in the reproductive organs of cotton, affecting the development of flower organs and sperm cells, so this family may play an important role in the reproductive development progress of cotton, and even in inducing cotton to produce haploid.
Annular unidirectional slip Couette flow with heat transfer and variable viscosity
Authors: Ali Ahmed E.K. , Abou-Dina M. S. , Ghaleb A. F. , Helal M. A. 
Published: April 2023

Abstract
This paper investigates the thermal, variable viscosity axial Couette flow between two con-centric circular cylinders, taking into account both the Navier and the dynamical slip boundary conditions. The nonlinear governing equations for momentum and energy balance are solved under start-up condition using the Laplace transform (LT) technique, in conjunction with the Homotopy Perturbation Method (HPM). The first two orders of approximation for tempera- ture and velocity are obtained, as well as the entropy generation in the space occupied by the flow and the volumetric flux. Numerical results and graphical representations of the solution are provided and discussed to depict the effects of the slip parameters on the velocity and the temperature profiles, on the entropy generation rate and on the volumetric flux.
Up against the wall - emergency double myocardial rupture repair
Authors: Ravi Rajeevlochan , Sinha Shubhra , Dunlop Craig , Unsworth-white Jonathan 
Published: April 2023

Abstract
Background
Left ventricular free wall rupture (LVFWR) and interventricular septal rupture (VSR) are potentially catastrophic mechanical complications after acute myocardial infarction (AMI). When they occur together, “double myocardial rupture” (DMR), survival is unlikely. DMR is seen in only 0.3% of all AMIs. With or without surgical intervention, the odds are against the patient.
Case presentation
A 57-year-old male self-referred to the emergency department of a remote hospital 5 days after first experiencing chest pain. Investigations in ED confirmed an inferior ST-segment elevation myocardial infarction (STEMI) complicated by DMR. Coronary angiography revealed a mid-course total occlusion of the right coronary artery (RCA).
He was rapidly transferred to our regional cardiac surgical unit, arriving straight into the operating theatre, in cardiogenic shock. He was briefly conscious, before arresting prior to intubation and being massaged onto bypass. Not only did he survive the all-night operation, requiring a mitral valve replacement in the process, but he survived multiple postoperative complications to be eventually transferred on postoperative day 66, neurologically intact, to a peripheral unit to complete his rehabilitation. He was subsequently discharged home 88 days after the operation and was able to ambulate with a walking frame into his first postoperative follow-up clinic appointment.
Conclusions
Our patient, against all odds, has survived DMR and multiple postoperative complications. We present the details of his case and the literature surrounding the condition. The patient’s mental fortitude and his supportive family played a big part, along with excellent team working in our unit.
Romeo and Juliet Meeting in Forest Like Regions
Authors: Misra Neeldhara , Mulpuri Manas , Tale Prafullkumar , Viramgami Gaurav 
Published: April 2023

Abstract

The game of rendezvous with adversaries is a game on a graph played by two players: Facilitator and Divider. Facilitator has two agents and Divider has a team of k ≥ 1 agents. While the initial positions of Facilitator’s agents are fixed, Divider gets to select the initial positions of his agents. Then, they take turns to move their agents to adjacent vertices (or stay put) with Facilitator’s goal to bring both her agents at same vertex and Divider’s goal to prevent it. The computational question of interest is to determine if Facilitator has a winning strategy against Divider with k agents. Fomin, Golovach, and Thilikos [WG, 2021] introduced this game and proved that it is PSPACE-hard and co-W[2]-hard parameterized by the number of agents.
This hardness naturally motivates the structural parameterization of the problem. The authors proved that it admits an FPT algorithm when parameterized by the modular width and the number of allowed rounds. However, they left open the complexity of the problem from the perspective of other structural parameters. In particular, they explicitly asked whether the problem admits an FPT or XP-algorithm with respect to the treewidth of the input graph. We answer this question in the negative and show that Rendezvous is co-NP-hard even for graphs of constant treewidth. Further, we show that the problem is co-W[1]-hard when parameterized by the feedback vertex set number and the number of agents, and is unlikely to admit a polynomial kernel when parameterized by the vertex cover number and the number of agents. Complementing these hardness results, we show that the Rendezvous is FPT when parameterized by both the vertex cover number and the solution size. Finally, for graphs of treewidth at most two and girds, we show that the problem can be solved in polynomial time.
2012 ACM Subject Classification Mathematics of computing → Discrete mathematics; Theory of computation → Design and analysis of algorithms
Oral Health Knowledge, Attitude and Behavior among Dental &amp; Medical Students of Kabul University of Medical Sciences
Authors: Ehsan Hedayatullah , Ahmadzai Nazera , Orfani Zarlasht , Rezayee Bibi Marwa , Wally Moqadasa , Daftani Sardara 
Published: April 2023

Abstract
Objective The study's goal was to find out how much dentistry and medical students at Kabul University of Medical Sciences knew about oral health, as well as their attitudes and behavior toward dental health and oral hygiene routines.Materials and Methods From September 2022 to December 2022, cross-sectional research was conducted at Kabul University of Medical Sciences. A total of 496 dentistry and medical students (312 females + 184 males) aged 18–25 years old from 5 colleges of health and medical related disciplines were addressed. All participants were given a thorough questionnaire with 30 amended items modified from Peterson et al. and Stenberg et al.Results 44.5% of the participants brushed their teeth twice per day, 41.3% once per day, and 9.31% more than twice per day, while just 4.7% cleaned their teeth less than once per day. Surprisingly, the majority of participants (91%) were aware of the significance of routine dental visits which 51.3% of participants reported visiting the dentist frequently even while they experienced pain. 94% of students had the opinion that brushing teeth prevents dental decay. 93% of them agreed that using of fluoride strengthens teeth and 96% polled that sweets are bad for teeth.Conclusions Oral health knowledge, attitudes, and behavior is associated with the individual subject’s educational level. Females are more concerned about their oral health than males. And for having better oral hygiene, there is need for educational programs for public.
Metabolic Dysfunction-Associated Fatty Liver Disease and Incident Heart Failure Risk: The Kailuan Cohort Study
Authors: Wei Zhihao , Huang Zhe , Song Zongshuang , Zhao Wenliu , Zhao Dandan , Tan Yizhen , Chen Shuohua , Yang Peng , Li Yun , Wu Shouling 
Published: April 2023

Abstract
Background
Recently, metabolic dysfunction-associated fatty liver disease (MAFLD) has been proposed to replace non-alcoholic fatty liver disease (NAFLD) to emphasize the pathogenic association between fatty liver disease and metabolic dysfunction. Studies have found that MAFLD independently increases the risk of myocardial infarction and stroke. But the relationship between MAFLD and heart failure (HF) is not fully understood.
Objectives
This study aimed to explore the association between MAFLD and the risk of HF.
Methods
The study included 98,685 participants without HF selected from the Kailuan cohort in 2006. All participants were divided into non-MAFLD group and MAFLD group according to MAFLD diagnostic criteria. After follow-up until December 31, 2020, the Cox regression analysis model was used to calculate the effect of MAFLD on the risk of HF.
Results
During the median follow-up of 14.01 years,3260 cases of HF were defined, the HF incidence density of non-MAFLD group and MAFLD group was 2.19/1000pys and 3.29/1000pys, respectively. Compared with the non-MAFLD group, participants with MAFLD had an increased risk of HF (HR: 1.40, 95% CI: 1.30-1.50); in addition, an exacerbation of fatty liver disease was associated with an increased risk of HF in people with MAFLD. We also observed a higher risk of HF among the different metabolic dysfunction of MAFLD in people with both fatty liver disease and type 2 diabetes (HR, 1.96; 95% CI, 1.73-2.21).
Conclusions
Our findings suggest that the risk of HF was significantly increased in participants with MAFLD, and an exacerbation of fatty liver disease was associated with an increased risk of HF in people with MAFLD. In addition, we should pay more attention to people with MAFLD with type 2 diabetes.
On the Poincare-Bendixson formula for planar piecewise smooth vector fields
Authors: Li Shimin , Liu Changjian , Llibre Jaume 
Published: April 2023

Abstract
The topological index, or simply the index, of an equilibrium point of a differential system is an integer which saves important information about the local phase portrait of the equilibrium.
There are mainly two ways to calculate the index of an isolated equilibrium point of a smooth vector field. First Poincare and Bendixson proved that the index of an equilibrium point can be obtained from the number of hyperbolic and elliptic sectors that there are in a neighborhood of the equilibrium point, which is known as Poincare-Bendixson formula for the topological index of an equilibrium point. Second several works contributed to the algebraic method of Cauchy's index for computing the index of an equilibrium point.
In this paper we extend the Poincare-Bendixson formula to planar piecewise smooth vector fields. Applying this formula we define the index of generic codimension-one equilibria for piecewise smooth vector fields, including boundary equilibria, pseudo-equilibria and tangency points.
Severe Mental Illness and Non-specific Abdominal Pain in the Emergency Department: A Multi-institutional Database Study
Authors: Chien Wei-Che , Kou Hao-Wei , Wu Kai-Hsiang , Gao Shi-Ying , Ng Chip-Jin , Hung Yu-Yung , Lee Chao-Wei , Li Chih-Huang , Hung Shang-Kai 
Published: April 2023

Abstract
Introduction: Abdominal pain is a common cause of emergency department (ED) visits, and non-specific abdominal pain (NSAP) accounts for a large proportion of diagnoses. Patients with severe mental illness (SMI) are particularly vulnerable due to their atypical disease presentation and high comorbidity rates. Previous studies have reported higher ED revisit rates and delayed diagnoses in patients with SMI and NSAP. This study aimed to evaluate ED management, unscheduled ED revisit rates, and short-term adverse outcomes in patients with SMI and NSAP.Methods: Relying on the Chang Gung Research Database (CGRD), we selectively used data from January 1, 2007, to December 31, 2017. Diagnoses of NSAP and SMI were confirmed by combining the ICD codes with relevant medical records. The non-SMI group was matched at a ratio of 1:3 using a Greedy algorithm. The outcomes were ED management, 72-hour unscheduled ED revisits, and 7-day adverse events.Results: A total of 233,671 patients from seven hospitals over a span of 11 years were recruited; among them, 98,722 were excluded based on the inclusion criteria, leaving 134,949 patients for analysis. The SMI group had more comorbidities, a higher rate of 72-hour unscheduled ED revisits, and was more likely to receive analgesics, but less likely to undergo laboratory tests and CT scans. Patients without SMI were more likely to be admitted to the ward and undergo invasive abdominal procedures within seven days after index ED discharge. No significant differences were found in ICU admission, abdominal surgery, or in-hospital mortality between the two groups.Conclusion: Our study demonstrated that patients with SMI and NSAP had a higher rate of 72-hour unscheduled ED revisits, but this did not lead to higher short-term adverse outcomes. Although NSAP is considered a safe diagnosis for both the general population and patients with SMI, the higher rate of unscheduled ED revisits suggests the need for better healthcare interventions to eliminate health disparities in this vulnerable group.
Long-term changes in precipitation in the eastern portion of the Guanabara Bay hydrographic region, Rio de Janeiro, Brazil
Authors: Abreu Marcel Carvalho , de Oliveira-Júnior José Francisco , Souza Amaury , Lyra Gustavo Bastos , Fraga Micael de Souza , Silva Felipe Bernardes da , Almeida Laura Thebit , Silva Marcos Vinícius da , Cecílio Roberto Avelino 
Published: April 2023

Abstract
The Guanabara Bay hydrographic region (GBHR) is a strategic area of Rio de Janeiro, with few studies on Climatology and on the annual maximum daily rainfall (Pmax). Detecting changes in Pmax is a prerequisite for a better understanding of the climate and developing adaptation and mitigation measures at a regional and local scale. Therefore, the aim of this study is to identify trends in Pmax through non-parametric tests in the GBHR region of Rio de Janeiro based on historical time series. Five rain gauges were selected with daily rainfall series due to long time series (ranging from 1939 to 2020) and with a maximum of 10% annual failures. The non-parametric tests (MK, modified MK and Pettitt) were applied to the rainfall temporal series. The series of Pmax were classified as random, independent, homogeneous and stationary. The Pmax were observed mostly between November and March. It is noteworthy that this identified period corresponds to the greater performance of South Atlantic Convergence Zone (SACZ), convective rainfall and Frontal Systems (FS) in Rio de Janeiro. The results of MK test were coincident with Pettitt test. The modified MK test detected a trend in rain gauges where the Pettit test was no significative. The change points by Pettit test, in significative trend were 1995 and 1985, for Nova Friburgo and Cachoeiras de Macacu. The results obtained from the study are important for territorial planning, airport management, and for the socioeconomic interest of GBHR – Rio de Janeiro, mainly for COMPERJ (petrochemical complex) and landslide and flood areas.
Contrasted avian communities between two protected areas in the metropolitan area of Port-au-Prince, Haiti
Authors: Exantus Jean-Marry , Cambrone Christopher , Bezault Etienne , Cézilly Frank 
Published: April 2023

AbstractAlthough urban and suburban protected areas may contribute to conserving biodiversity, their importance remains poorly documented. We compared the composition of bird assemblages between two protected areas, the Urban National Park of Martissant (UNPM) and the suburban Wynne Farm Ecological Reserve (WFER) in Haiti, one the poorest country in the world, plagued with severe deforestation and environmental degradation. Species were identified along transects without distance estimation. A total of 718 birds belonging to 38 different species were censused (494 at UNPM and 224 at WFER, from 24 and 26 species, respectively), including two endemic and vulnerable species,Corvus leucognaphalusandAmazona ventralis, observed only at the urban site. Species richness, diversity, evenness and species-abundance distributions did not differ significantly between sites. However, the composition of the two avian assemblages differed significantly, with only 31.6% species in common and a significant difference in their relative abundance. We found no evidence for a difference in the degree of forest dependency between the two assemblages. However, mainly and strict insectivorous species accounted for a larger proportion of observations at WFER (34.8%) compared to UNPM (19.6%). Factors influencing the contrast in avian assemblage composition between two sites include differences in elevation and associated vegetation, difference in water availability and variation in the ability of species to adapt to the urban environment. Our results demonstrate the importance and complementarity of the two protected areas for the conservation of avian diversity in Haiti.
A Persistent Median Artery Piercing the Median Nerve: A Review and Clinical Implications
Authors: Pavlovsky Ethan , Kistner Gilberto , Loomis Mario 
Published: April 2023

Abstract
Purpose: This case report documents a rare variation of a persistent median artery. Normally a transient vessel present during embryologic development of the limbs, the median artery typically regresses by the eighth week of life as the radial and ulnar arteries predominate the distal vascular supply of the hand. In a subset of the adult population, this artery remains as a persistent median artery which most often ends in the carpal tunnel or hand without significant vascular dependence.
Methods: We report a unique variant of the persistent median artery that perforates the median nerve and contributes a significant portion of the blood supply to the hand.
Results: An 84-year-old female cadaver was found bilaterally to have an artery that penetrated the median nerve in the forearm, continued through the carpal tunnel, then joined the superficial arch, giving rise to the common digital artery to the second webspace. It also gave rise to the radialis indicis, the princeps pollicis, and an anastomotic branch to the radial artery.
Conclusion: This anatomic variant of a persistent median artery piercing the median nerve, traversing the carpal tunnel, and providing aberrant primary blood supply to a large area of the hand are details clinically relevant to those who treat hand disorders and injuries.