bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic value of high-risk HPV E6/E7 mRNA in patients with ASCUS Analysis of pathogenicity of HPV in patients with ASCUS
Authors: Jin xiu , Liu Feifei , Zhang Ya , Ma Yingying , Yang Linqing , Wang Yunfei , Liu Ying 
Published: May 2023

Abstract
Objective: To investigate the infection status of high-risk human papillomavirus (HR-HPV) E6/E7 mRNA in patients with a cytological diagnosis of "atypical squamous cells of undetermined significance" (ASCUS) and to analyze the pathogenic rate of different high-risk HPV subtypes combined with biopsy pathological results to provide a more accurate basis for managing ASCUS patients.
Methods A total of 1387 patients with ASCUS and HPV E6/E7 mRNA positivity who were referred for colposcopy were retrospectively analyzed. They were divided into HPV16+, 18/45+ and other HR-HPV+ groups premenopausal and postmenopausal groups. The pathological results of the biopsy were divided into the LSIL- group (including normal and low-grade squamous intraepithelial lesions) and the HSIL+ group (including high-grade squamous intraepithelial lesions and higher lesions). SPSS was used for the analysis.
Results The age group 31-40 years had the highest level of HPV16+, and HPV18/45+ was the highest in the 41-50 years group. The detection rates of HSIL+ in the HPV16+, HPV18/45+, HPV 16/18/45+ and Other HR-HPV+ groups were 48.4%, 18.8%, 43.9% and 15.0%, respectively. The infection rates of HPV16/18/45 in postmenopausal and premenopausal women were 42.4% and 34.3%, respectively. In the HPV18/45 group, the incidence of HSIL+ was 30.0% in postmenopausal women and 15.0% in premenopausal women (P < 0.01). In the HPV 16+ and Other HR-HPV+ groups, the incidence of HSIL+ in postmenopausal patients was not significantly different from that in premenopausal patients. The incidence of cervical cancer in postmenopausal patients is significantly higher than that in premenopausal patients.
Conclusions Colposcopy referral or further biopsy is recommended for all ASCUS patients with HPV16/18/45E6/E7 mRNA positivity and postmenopausal patients with HR-HPVE6/E7 mRNA positivity. For premenopausal ASCUS patients with other HR-HPV E6/E7 mRNA positivity, colposcopy should be performed if possible, depending on the specific situation, to achieve early detection and diagnosis.
Effects of the 1RS.1BL wheat-rye translocation on kernel and bran content of bread wheat
(Triticum aestivum L.)
Authors: Aydın Nevzat , Demir Bedrettin , Sayaslan Abdulvahit , KÖSE Özge Doğanay ERBAŞ , Güleç Tuğba , Şermet Cemal , Savaşlı Erdinç , Sönmez Mesut Ersin , Koyuncu Mehmet , MUT Zeki 
Published: May 2023

Abstract
Rye introgressions are important contributors to the genetic diversity of wheat. In this study, we investigated the effects of a wheat-rye translocation 1RS.1BL in bread wheat on kernel and bran properties and mineral content. The plant material consisted of 147 recombinant inbred lines (RILs) and five control cultivars, including the parent. The genotypes were grown in Turkey at two different locations for two consecutive years.The protein, starch, fat, ash, dry matter, acid detergent insoluble fiber (ADF), neutral detergent insoluble fiber (NDF), potassium (K), phosphorus (P) and magnesium (Mg) contents of kernels and bran of the RILs were determined. Statistically significant differences were found between the genotypes in terms of all the examined characteristics except the amount of dry matter. The RILs carrying translocation 1RS.1BL had a higher average ADF, NDF, protein content, ash content, K, Mg, and P contents of kernels, as well as a higher average ADF, NDF, ash, and K bran contents than the RILs without the translocation. The bran of the RILs without the translocation had higher values in terms of fat, starch, and phosphorus content. The results indicate that wheat- rye translocation 1RS.1BL has a significant effect on both whole wheat kernel and bran content, and can be used to enrich the content of wheat bran.
Launching of several small- scale successive jets from calcium bright knots and formation of chromospheric anemone jets on the Sun
Authors: Singh Kunwar Alkendra Pratap , Nishida Keisuke , Shibata Kazunari 
Published: May 2023

Abstract
Recent space solar observations revealed that solar atmosphere such as corona and chromosphere is full of small- scale jets1, associated with small- scale explosions called nanoflares. There is increasing indirect evidence that these jets may be produced by the physical mechanism similar to that of large scale flares, i.e., release of magnetic energy stored in the solar atmosphere through magnetic reconnection2-4, and also that these jets and nanoflares may be related to the heating of corona and chromosphere as well as acceleration of solar wind5,6. Among small scale jets, the so called chromospheric anemone jets in the active region on the Sun remains puzzling, because they are one of the smallest scale jets so that their footpoints (bright knots) have not been well resolved7,8,9. We propose a new model for chromospheric jets using the three-dimensional magnetohydrodynamic (MHD) simulations, which show that the continuous, upward rising of small-scale twisted magnetic flux rope in a magnetized solar chromosphere play an important role in small-scale magnetic reconnections and launching of several small-scale jets during the evolution of the chromospheric anemone jets. Our new, self-consistent, three-dimensional computer modeling of small-scale, but ever-changing flux rope emergence in the magnetized solar atmosphere is found to be fairly consistent with observations.
Encoding Beacon Statements for Code Comment Generation
Authors: Jia Nan , Chen Jie , Li Mingliang 
Published: May 2023

Abstract
High-quality code comment is important to help developer review and comprehend source code. Inspired by the effectiveness of deep learning techniques in the NLP field, many studies focus on using the machine translation algorithm to automatically generate comment for the source code. Most of the current studies typically involve in collecting a large number of dataset regarding the function-comment pairs, and then encode the information (e.g., AST) from the whole function for training a generation model. Most of current studies indistinguishably encode every statement in a function, which may not be the best strategy for comment generation because each statement in a function plays different roles, and the non-core code statements may have no effect or even a negative effect on the comment generation. In this paper, we propose a flexible encoding strategy that highlights the most informative statements from the function, called beacon statements, for code comment generation. Specifically, the beacon statements are detected from the function according to their roles when human understanding program, and then a pre-training model is proposed to encode the knowledge of the beacon statements. At last, the knowledge of the beacon statements is transferring to a basic model for comment generation by sharing the training parameters. The encouraging experimental results demonstrate the feasibility and effectiveness of our comment generation model.
Application of 3D printing for bioreactor prototype development
Authors: Santos Robson Marino Garcia , Rodrigues Leonardo Ribeiro 
Published: May 2023

Abstract
Growing a patient's bone cells in custom-made porous support structures (scaffolds) has been considered as a future alternative to reduce the risks associated with bone transplants and simplify this procedure. To make it viable, 3D printing has been studied for bioreactor production, a fundamental equipment for the development of this technique, but expensive and difficult to manufacture. So, after a detailed literature review, this article compiles requirements for the design of new bioreactors and parameters for its validation, applying them in the development of a prototype to be manufactured through 3D printing. The project was developed and evaluated through simulations performed with Solidworks software, considering the shear stress generated in an 8 mm3 reference scaffold model with 400 µm cubic pores. The proposed designs are different from others identified in the literature review and presented average results within the range defined as adequate, from 0.11 to 30 mPa. No similar study, compiling information and presenting parameters with the same level of detail, was identified. Future studies should focus on design adjustments to improve flow perfusion homogeneity and on laboratory tests.
Two-dimensional inerter-enhanced nonlinear energy sink
Authors: Yang Tianzhi , Dang Wenhu , Chen Liqun 
Published: May 2023

Abstract
Multiple-direction vibration widely exists in the environment, which is harmful to instruments and people. Hence, the high demand for vibration isolators with exceptional efficiency is thus evident. Although the proposed nonlinear energy sink was proven to be highly utility, most of the developed such devices, even with some inerter enhanced NESs, are limited to one-dimensional vibration suppression. In this paper, we propose a two-dimensional inerter-enhanced NES (2D IE-NES) which is applicable for multiple-directional low-frequency vibration suppression. The Lagrangian method is employed to derive the dynamic equations of the 2D IE-NES. Then these differential equations are solved by means of the Runge-Kutta method. Numerical results show that the novel 2D IE-NES configuration can isolate vibration more efficiently than the traditional NES under both instantaneous shock and constant periodic excitation. The role of the inerter in the vibration decaying process is highlighted in energy flow. This paper provides a new platform for the low-frequency multiple-direction vibration isolator.
Fundamentals and implication of PZC determination for activated carbons in aqueous electrolytes
Authors: Fic Krzysztof , Sr Sylwia , Galek Przemyslaw , Menzel Jakub , Platek-Mielczarek Anetta 
Published: May 2023

Abstract

The point of zero charge (PZC) is crucial for investigating molecular level charging mechanisms in energy storage systems, as demonstrated in electrochemical capacitors. Three electrochemical techniques were studied: cyclic voltammetry (CV), staircase potentio electrochemical impedance spectroscopy (SPEIS) and step potential electrochemical spectroscopy (SPECS) for two activated carbons (ACs) with 0.1 mol L− 1 aqueous solutions of LiNO3, Li2SO4, and KI. The porous AC charging process in aqueous electrolytes is a complex phenomenon; the ion mixing zone covered a wide potential region. Inadequate PZC determination could lead to obscure data evaluation, which could further provide a misguided mechanism description at the molecular level. In the aqueous solutions studied, the adsorption of specific ions and active participation of all ionic species in the electrical double-layer formation were considered. The SPECS technique was determined to be the most beneficial for the PZC determination in electrochemical quartz crystal microbalance applications.
Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes
Authors: Masaki Kanako , Ahmed Abo Bakr F. , Ishida Takenori , Funabashi Hisakage , Hirota Ryuichi , Ikeda Takeshi , Kuroda Akio 
Published: May 2023

Abstract
Purpose: The use of small extracellular vesicles (sEVs) such as exosomes for therapeutic purposes has recently generated great interest. Developing an efficient, rapid, scalable, and reproducible sEV purification method is necessary. Therefore, this study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could potentially meet this demand.
Methods: We used the ExoPUA column connected to a fast-performance liquid chromatography system.
Results: One-step chromatographic purification of sEVs from a culture supernatant using the ExoPUA protocol resulted in an 82-fold increase in purity with a yield of 38% of sEVs on average. Transmission electron microscopy and western blotting analysis indicated that purified sEVs were intact.
Conclusion: The purification performance of the ExoPUA protocol yields superior results compared with that of the differential ultracentrifugation method, which is the most commonly used purification method for sEVs in the laboratory. Furthermore, sEVs were purified in the bind-elute mode and the ExoPUA column can be refreshed and sterilized with sodium hydroxide (NaOH), having more potential for multiple sEV purification in a scalable and industrial manner.
IOT Based Automatic Intravenous Bag Data Logging Monitoring and Alert System
Authors: SRI S , RAJESHKUMAR G , SARANYA E , SARANYA K , KAVITHA R 
Published: May 2023

Abstract
Saline, one of the most widely used intravenous (IV) medications, is essential in the treatment of critically ill patients. Monitoring the level in the saline bottle is crucial because blood flows when the bottle is empty and the needle is still near the vein into the bottle from the outside. In hospitals, it is the responsibility of nurses or caregivers to monitor level of saline bottle. The proper timing of withdrawing the needle from the patient's vein is usually ignored due to carelessness and any unusual circumstances that result in great casualties and should also lead to death. In addition, health services can be offered through remote monitoring. To avoid the accident caused by caregiver ignorance and offer remote, we designed a cost-effective intelligent saline level for supervision in health services. a monitoring system that combines sensor and Internet of Things (IOT) technology. This system was created using load cell and a cheap, very low power microcontroller. The load of the bottle is converted to the selected voltage by the load sensor. The ESP8266 microcontroller generates and publishes a specific message based on voltage received from the sensor. Publish and display messages on subscriber devices like health professionals, nurses or caregivers. The proposed monitoring system delivers message to subscribers in a reliable manner, which is crucial for the healthcare industry. With the help of a human, the valve of the saline bottle closes automatically
Highly conductive and stretchable hydrogel using a percolated network of whiskered gold nanosheets
Authors: Hyeon Taeghwan , Lim Chaehong , Lee Seunghwan , Park Chansul , Kim Jeong Hyun , Kim Dae-Hyeong 
Published: May 2023

Abstract
Conductive and stretchable hydrogels (CSHs) are promising materials for soft bioelectronics. However, hitherto developed CSHs suffer from unsatisfactory electrical conductivity and stretchability. As the electrical properties of a CSH are determined by the type of conductive components and the quality of their percolation, a breakthrough in these factors is required for the high performance CSH. Here, we developed CSHs including a percolated network of whiskered gold nanosheets (wAu-CSHs). A high fraction of whiskered gold nanosheets (> 3 vol.%) is impregnated in the hydrogel matrix through a sequential formation process of the gold nanomaterial network and the hydrogel matrix. This wAu-CSH fabrication method is applicable to various hydrogels includingpolyacrylamide, polyacrylic acid, and polyvinyl alcohol, and even to an organogel such as polybutyl acrylate. Regardless of the types of hydrogels, the wAu-CSH exhibits a conductivity of ~ 500 S/cm and a maximum strain of ~ 300 % evenwithout any supporting substrate. We increased the density of the gold nanomaterial network through a pressing process to maximize the conductivity of wAu-CSHs,exhibitinga high conductivity of ~ 3300 S/cm and a maximum strain of ~ 100% with a supporting hydrogel layer.
Mother Optimization Algorithm: A New Human-Based Metaheuristic Approach for Solving Engineering Optimization
Authors: Matoušová Ivana , Trojovský Pavel , Dehghani Mohammad , Trojovská Eva , Kostra Juraj 
Published: May 2023

Abstract
This paper presents a new metaheuristic method called Mother Optimization Algorithm (MOA) that mimics the human interaction between a mother and her children. The fundamental inspiration of MOA is to simulate the mother's care of children in three phases education, advice, and upbringing. The mathematical model of MOA used in the search process and exploration is presented. The performance of MOA is assessed on a set of 52 benchmark functions, including unimodal and high-dimensional multimodal functions, fixed-dimensional multimodal functions, and the CEC 2017 test suite. The results indicate that MOA's proposed approach, which balances exploration and exploitation, effectively supports the search process and can generate appropriate solutions for optimization problems. The outcomes quality obtained from MOA has been compared with the performance of twelve often-used metaheuristic algorithms. Upon analysis and comparison of the simulation results, it was found that the proposed MOA outperforms competing algorithms with superior and significantly more competitive performance. Specifically, the proposed MOA delivers better results in most objective functions. Furthermore, the application of MOA on four engineering design problems demonstrates the efficacy of the proposed approach in solving real-world optimization problems.
Alfosbuvir plus Daclatasvir for Treatment of Chronic Hepatitis C Virus Infection in China
Authors: Hua Rui , Kong Fei , Li Guangming , Wen Xiaofeng , Zhang Yuexin , Yang Xingxiang , Meng Chenxin , Xie Wen , Jiang Yongfang , Wang Xiaozhong , Han Xueji , Huang Yan , Mao Qing , Wang Jiefei , Guan Yujuan , Chen Jiayu , Ma Yingjie , Xiong Qingfang , Ma Hong , Yan Xuebing , Rao Huiying , Zhao Yingren , Sun Tong , Zhu Liying , Mao Xiaorong , Lian Jianqi , Deng Guojiong , Xin Yongning , Wang Yifei , Ye Yinong , Xu Bin , Gao Hainv , Tan Youwen , Li Dongliang , Yang Dongliang , Su Minghua , Zhang Xiaomeng , Min Jie , Shi Xinsheng , Wei Lai , Niu Junqi 
Published: May 2023

Abstract
Background and aims
A pan-genotypic and effective treatment regimen for patients with chronic hepatitis C virus (HCV) infection remains an unmet medical need in China. Alfosbuvir is a novel potent HCV NS5B polymerase inhibitor in development for the treatment of chronic HCV infection. We conducted a phase 3 study to evaluate the efficacy and safety of alfosbuvir in combination with daclatasvir in Chinese patients with HCV infection.
Methods
All patients received 600 mg alfosbuvir tablets plus 60 mg daclatasvir tablets once daily for 12 weeks. The primary endpoint was a sustained virologic response 12 weeks after the end of treatment (SVR12). A follow-up visit was done at week 4 and 12, and those who achieved SVR12 were followed up at post-treatment week 24.
Results
Of the 326 patients who received at least one dose of the study treatment, 321 (98.5% [95% CI 96.5–99.5%]) achieved SVR12, which was superior to the historical SVR12 rate of 88% (p < 0.0001). The SVR12 rates were similar regardless of most baseline characteristics. The most common adverse event (≥ 10%) was hypercholesterolemia. Serious adverse events (SAEs) were reported in 25 (7.7%) patients, none of which was judged to be related to the study treatment. The majority of adverse events (AEs) were mild to moderate in severity.
Conclusions
Alfosbuvir plus daclatasvir for 12 weeks was highly effective and safe in Chinese patients infected with HCV genotype 1, 2, 3, or 6, suggesting this regimen could be a promising option for HCV treatment in China irrespective of genotype.
Perturbation and stability of PAM50 subtyping in population-based primary invasive breast cancer
Authors: Staaf Johan , Veerla Srinivas , Hohmann Lennart , Nacer Deborah , Vallon-Christersson Johan 
Published: May 2023

Abstract
PAM50 gene expression subtypes represent a cornerstone in molecular classification of breast cancer and are included in risk prediction models to guide therapy. We aimed to illustrate the impact of included genes and biological processes on subtyping while considering a tumor’s underlying clinical subgroup defined by ER, PR and HER2 status. To do this we used a population-representative and clinically annotated primary breast tumor cohort of 6233 samples profiled by RNA sequencing and applied a perturbation strategy of excluding co-expressed genes (gene sets). We demonstrate how PAM50 nearest centroid classification depends on biological processes present across, but also within, ER/PR/HER2 subgroups and PAM50 subtypes themselves. Our analysis highlights several key aspects of PAM50 classification. Firstly, we observed a tight connection between a tumor’s nearest and second nearest PAM50 centroid. Additionally, we show that second-best subtype is associated with overall survival in ER-positive, HER2-negative, and node negative disease. We also note that ERBB2 has little impact on PAM50 classification in HER2-positive disease regardless of ER-status, and that the Basal subtype is highly stable in contrast to the Normal subtype. Improved consciousness of the commonly used PAM50 subtyping scheme will aid in our understanding and interpretation of breast tumors that have seemingly conflicting PAM50 classification when compared to clinical biomarkers. Finally, our study adds further support in challenging the common misconception that PAM50 subtypes are distinct classes by illustrating that PAM50 subtypes in tumors represent a continuum that may have clinical implications.
Metasurface-enabled Broadband All-Optical Edge Detection in Visible Frequencies
Authors: Aydin Koray , Tanriover Ibrahim , Dereshgi Sina Abedini 
Published: May 2023

Abstract
Image processing is of fundamental importance for numerous modern technologies including AR/VR glasses, autonomous driving, and biometric identification. In recent years, due to increasing demand for real-time, continuous data processing, metamaterial and metasurface based all-optical computation techniques emerged as a promising alternative to digital computation. Most of the pioneer research focused on all-optical edge detection as a fundamental step of image processing. Metasurfaces have been shown to enable real time edge detection with low to no power consumption. However, the previous demonstrations were subjected to the several limitations such as need for oblique-incidence, polarization dependence, need for additional polarizers, narrow operation bandwidth, being limited with processing in 1D, operation with coherent light only, and requiring digital post-processing. Here, we propose and demonstrate metasurfaces for 2D isotropic and polarization independent edge detection based on Fourier optics principles that overcome aforementioned limitations and challenges. Our proposed metasurface platform consists of co-centric metallic rings that enable carefully chosen spatial transmission profile matching the Fourier transformation of the second-order differentiation. We experimentally confirm polarization-independent, broadband edge detection with high transmission efficiency under both coherent and incoherent illumination along the visible frequency range. Additional simulations and measurements in the near-IR wavelength range confirmed edge detection by the same metasurface, indicating broadband operation capability. Our approach addresses several challenging tasks at once and therefore paves the path towards the application of metasurfaces for real-life image processing and all-optical computation tasks.