bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intensified Adjuvant Treatment for High‐Risk Resected Cutaneous Angiosarcoma of the Head and Neck
Authors: El Shatanofy Muhammad , Thakkar Punam , Patel Vishal , Joshi Arjun , Goodman Joseph , Siegel Robert , Haroun Faysal , Ojong‐Ntui Martin , Goyal Sharad , Bauman Julie , Rao Yuan James 
Published: July 2023

AbstractObjectivePrevious studies have highlighted the poor survival of patients with cutaneous angiosarcoma of the head and neck. Therapeutic options are limited, and effective treatment strategies are yet to be discovered. The objective of this study is to evaluate overall survival following intensified adjuvant treatment for high‐risk resected angiosarcoma of the head and neck.Study DesignRetrospective observational.SettingNational Cancer Database (NCDB).MethodsPatients diagnosed with nonmetastatic cutaneous angiosarcoma of the head and neck from 2004 to 2016 were identified by NCDB. We retrospectively compared demographics and overall survival between patients who received surgery and radiation therapy (SR) and patients who received surgery and chemoradiation (SRC). The χ2 test, Kaplan‐Meier method, and Cox regression models were used to analyze data.ResultsA total of 249 patients were identified, of which 79.5% were treated with surgery and radiation alone and 20.5% were treated with surgery and chemoradiation. The addition of chemotherapy, regardless of the sequence of administration, was not associated with significantly higher overall survival. Factors associated with worse survival in both groups included positive nodal status and positive margins. Patients with positive nodes had higher overall survival with radiation doses >50.4 Gy compared to ≤50.4 Gy (hazard ratio: 2.93, confidence interval: 1.60‐5.36, p < 0.001).ConclusionAdjuvant chemotherapy was not significantly associated with higher overall survival for resected nonmetastatic angiosarcoma of the head and neck. Higher radiation doses appear to be prognostic for high‐risk diseases.
Coagulant potentials of emicizumab in the plasmas from infant and toddler patients with hemophilia A
Authors: Takeyama Masahiro , Matsumoto Naoki , Abe Hiroto , Harada Suguru , Ogiwara Kenichi , Furukawa Shoko , Shimonishi Naruto , Nakajima Yuto , Yada Koji , Soeda Tetsuhiro , Nogami Keiji 
Published: July 2023

AbstractBackgroundEmicizumab significantly reduces bleedings in patients with hemophilia A (PwHA). A clinical study (HAVEN 7; NCT04431726) for PwHA aged less than or equal to 12 months is ongoing, but emicizumab‐driven coagulation potential in PwHA in early childhood remains to be clarified.AimTo investigate the in vitro or in vivo coagulation potential of emicizumab in plasmas obtained from infant and toddler PwHA.MethodsTwenty‐seven plasma samples from 14 infant/toddler PwHA (aged 0–42 months, median 19 months) who received emicizumab (n = 9), factor (F)VIII products (n = 8), or no treatment (n = 10) were obtained. FVIII activity in FVIII‐treated plasmas was cancelled by the addition of anti‐FVIII monoclonal antibody (mAb). Emicizumab‐treated plasmas (in vivo) and emicizumab‐spiked plasmas (in vitro) were analyzed. Emicizumab‐untreated plasma or emicizumab‐treated plasma supplemented with two anti‐emicizumab mAbs were used as references. Adjusted maximum coagulation velocity (Ad|min1|) by clot waveform analysis and peak thrombin (Peak‐Th) by thrombin generation assay was assessed.ResultsAd|min1| values in 24 samples were improved by the presence of emicizumab. Values did not improve in the three remaining samples (aged 1, 23, and 31 months). Although the presence of emicizumab showed an age‐dependent increase in Peak‐Th in 20 samples, this increase was not observed in seven samples (aged 0, 1, 1, 2, 8, 19, and 36 months). Emicizumab‐dependent increases in both Ad|min1| and Peak‐Th were shown in 18 samples, and increases in either parameter were shown in eight samples. One sample (from patient aged 1 month) showed no increase in both, however.ConclusionEmicizumab could improve coagulant potential in plasmas from infant/toddler patients with hemophilia A.
Endophytic Beauveria bassiana induces biosynthesis of flavonoids in oilseed rape following both seed inoculation and natural colonization
Authors: Muola Anne , Birge Traci , Helander Marjo , Mathew Suni , Harazinova Vili , Saikkonen Kari , Fuchs Benjamin 
Published: July 2023

AbstractBackgroundCultivation of oilseed rape Brassica napus is pesticide‐intensive, and alternative plant protection strategies are needed because both pesticide resistance and legislation narrow the range of effective chemical pesticides. The entomopathogenic fungus Beauveria bassiana is used as a biocontrol agent against various insect pests, but little is known about its endophytic potential and role in plant protection for oilseed rape. First, we studied whether B. bassiana can establish as an endophyte in oilseed rape, following seed inoculation. To evaluate the plant protection potential of endophytic B. bassiana on oilseed rape, we next examined its ability to induce plant metabolite biosynthesis. In another experiment, we tested the effect of seed inoculation on seedling survival in a semi‐field experiment.ResultsBeauveria bassiana endophytically colonized oilseed rape following seed inoculation, and, in addition, natural colonization was also recorded. Maximum colonization rate was 40%, and generally increased with inoculation time. Seed inoculation did not affect the germination probability or growth of oilseed rape, but B. bassiana inoculated seeds germinated more slowly compared to controls. Endophytic colonization of B. bassiana induced biosynthesis of several flavonoids in oilseed rape leaves under controlled conditions. In the experiment conducted in semi‐field conditions, inoculated seedlings had slightly higher mortality compared to control seedlings.ConclusionBeauveria bassiana showed endophytic potential on oilseed rape via both natural colonization and seed inoculation, and it induced the biosynthesis of flavonoids. However, its use as an endophyte for plant protection against pests or pathogens for oilseed rape remains unclear. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Longitudinal efficacy and safety modeling and simulation framework to aid dose selection of belantamab mafodotin for patients with multiple myeloma
Authors: Collins Jon , van Noort Martijn , Rathi Chetan , Post Teun M. , Struemper Herbert , Jewell Roxanne C. , Ferron‐Brady Geraldine 
Published: July 2023

AbstractBelantamab mafodotin, a monomethyl auristatin F (MMAF)–containing monoclonal antibody‐drug conjugate (ADC), demonstrated deep and durable responses in the DRiving Excellence in Approaches to Multiple Myeloma (DREAMM)‐1 and pivotal DREAMM‐2 studies in patients with relapsed/refractory multiple myeloma. As with other MMAF‐containing ADCs, ocular adverse events were observed. To predict the effects of belantamab mafodotin dosing regimens and dose‐modification strategies on efficacy and ocular safety end points, DREAMM‐1 and DREAMM‐2 data across a range of doses were used to develop an integrated simulation framework incorporating two separate longitudinal models and the published population pharmacokinetic model. A concentration‐driven tumor growth inhibition model described the time course of serum M‐protein concentration, a measure of treatment response, whereas a discrete time Markov model described the time course of ocular events graded with the GSK Keratopathy and Visual Acuity scale. Significant covariates included baseline β2‐microglobulin on growth rate, baseline M‐protein on kill rate, extramedullary disease on the effect compartment rate constant, and baseline soluble B cell maturation antigen on maximal effect. Efficacy and safety end points were simulated for various doses with dosing intervals of 1, 3, 6, and 9 weeks and various event‐driven dose‐modification strategies. Simulations predicted that lower doses and longer dosing intervals were associated with lower probability and lower overall time with Grade 3+ and Grade 2+ ocular events compared with the reference regimen (2.5 mg/kg every 3 weeks), with a less‐than‐proportional reduction in efficacy. The predicted improved benefit–risk profiles of certain dosing schedules and dose modifications from this integrated framework has informed trial designs for belantamab mafodotin, supporting dose‐optimization strategies.
Dion–Jacobson to Alternating‐Cations‐Interaction Reconstruction toward Narrow Bandgap 2D Aromatic Hybrid Perovskite
Authors: Dai Hongliang , You Shihai , Ye Huang , Zhu Tingting , Luo Junhua 
Published: July 2023

AbstractThe 2D aromatic Dion–Jacobson (DJ) hybrid perovskites combining advantages of high stability, enhanced light absorption, and favorable charge transport, are regarded as a kind of very promising materials for high‐performance optoelectronic applications. However, due to the rigidity and large size of the aromatic ring, how to further reduce the interlayer distance to achieve better carrier transport and wider light response window still remain extremely challenging. Here, an interesting DJ‐to‐ACI (alternating‐cations‐interaction) reconstruction in 2D aromatic perovskite is first realized by inserting MA+ cations into (4‐AP)PbI4 (1, 4‐AP = 4‐amidinopyridinium), successfully constructing an unprecedented ACI perovskite of (4‐AP)(MA)2Pb2I8 (2, MA = methylamine). Remarkably, such a DJ‐to‐ACI reconstruction not only effectively reduces the interlayer spacing from 3.89 to 3.15 Å but also alleviates the structural distortion, which jointly causes a significant bandgap narrowing from 2.22 to 1.95 eV (smaller than all current 2D monolayered DJ perovskites), hence achieving a broad photodetection window over 660 nm. This work reports a novel narrow bandgap 2D ACI perovskite derived from the aromatic DJ motif, which sheds light on future regulations on the structure and properties of hybrid perovskites.
Attribute‐based encryption scheme for secure data sharing in cloud with fine‐grained revocation
Authors: Islam Md Azharul , Madria Sanjay 
Published: July 2023

AbstractAttribute‐based encryption (ABE) is a prominent cryptographic tool for secure data sharing in the cloud because it can be used to enforce very expressive and fine‐grained access control on outsourced data. The revocation in ABE remains a challenging problem as most of the revocation techniques available today, suffer from the collusion attack. The revocable ABE schemes which are collusion resistant require a semi‐trusted manager to update the secret keys of nonrevoked users in order to achieve revocation. This introduces computation and communication overhead, and also increases the overall security vulnerability. In this paper, we propose two collusion resistant revocable ABE schemes that do not require any semi‐trusted entity. Our first scheme supports revocation at the user‐level that is equivalent to revoking all the attributes from a user. Our second scheme supports revocation at the attribute‐level that enables more fine‐grained revocation by allowing selective attribute(s) revocation from a user. We call them user‐level revocable ABE (ULR‐ABE) and attribute‐level revocable ABE (ALR‐ABE), respectively. For both the schemes, the secret keys of the nonrevoked users are never affected and the decryption algorithm has the same performance as the baseline ABE scheme. We are able to achieve these at the cost of some increase (compared to the baseline scheme) in the size of the secret key and the ciphertext.
Luteal phase decrease in packed cell volume in healthy non‐pregnant and pregnant bitches
Authors: Moxon Rachel , Dutton Worsfold Rebekah , Davis Julie , Adams Wendy , England Gary C. W. 
Published: July 2023

AbstractObjectivesTo establish packed cell volume (PCV) ranges for non‐pregnant, pregnant and post‐partum bitches from day 10 of proestrus, investigating any relationship with parity and litter size.MethodsThis prospective cohort study used 37 healthy breeding bitches to examine PCV counts from routine blood samples collected every 4 weeks, from day 10 of proestrus, as part of routine PCV monitoring.ResultsFor pregnant (n = 19) and non‐pregnant (n = 18) bitches, PCV decreased until week 8 (corresponding to 8.5 ± 1.1 days before whelping for pregnant bitches) and recovered by 16–20 weeks after the initial sample; bitches that whelped average and large litters showed greater declines. PCV began to recover sooner for bitches that had previously whelped one or two litters compared to bitches that had previously whelped three or more litters. There was a significant three‐way interaction between time after the onset of proestrus, litter size and the number of previous litters which demonstrated that the large decrease in PCV for bitches that had previously whelped three or more litters only occurred in bitches that were expecting an average or large sized litter.Clinical SignificanceChronological variation in PCV for pregnant and non‐pregnant bitches was established during the reproductive cycle. There was no evidence to suggest that routine PCV measurement for normal, healthy bitches would be beneficial. However, knowledge from this study may be useful when deciding whether to prospectively monitor a bitch where there is a history of previous pregnancy‐related anaemia, when performing a caesarean section due to the anticipated blood loss during surgery, or when examining blood profiles for post‐litter bitches.
Comparison of spermatic cord ligation and the vas plexus ligation in canine orchiectomy: A prospective clinical study
Authors: Şenocak Mümin Gökhan 
Published: July 2023

AbstractBackgroundOrchiectomy with a vas deferens to pampiniform plexus ligation (VPL) is a novel method, and it is unclear how its short‐term outcomes compare with the results of a conventional method, spermatic cord ligation (SCL).ObjectiveTo compare the short‐term outcomes of SCL and VPL on inflammation, surgery time, bleeding, pain and surgeon satisfaction during canine open orchiectomy.MethodsThirty male crossbred dogs undergoing open orchiectomy were enrolled the study. Dogs were randomly allocated to one of the SCL or VPL groups, with 15 patients in each. In the SCL group, the spermatic cord was ligated using absorbable sutures. The vas deferens, and pampiniform plexus self‐tying were performed in the VPL group. Surgery time, bleeding and surgeon satisfaction scores were recorded. Inflammation at the surgical site was assessed using infrared thermal camera over three days, and pain associated with inflammation was scored on the third day.ResultsOn Day 3, the average temperature in the SCL group was significantly higher than that of the VPL group, with a mean difference of 4.63°C (95% CI: 2.34–6.93, p < 0.001). Moreover, the surgery time in the VPL group was significantly longer compared to the SCL group, with a mean difference of 1.7 min (95% CI: 0.28–3.11, p = 0.021). The bleeding score was also significantly higher in the VPL group (p = 0.012). On the other hand, surgeon satisfaction and pain scores were not significantly different between groups.ConclusionBoth SCL and VPL methods are safe and effective for orchiectomy in dogs. VPL is comparable in efficacy and safety and has the additional benefit of less inflammation.
Comparative study of PRPH2 D2 loop mutants reveals divergent disease mechanism in rods and cones
Authors: Ikelle Larissa , Makia Mustafa , Lewis Tylor , Crane Ryan , Kakakhel Mashal , Conley Shannon M. , Birtley James R. , Arshavsky Vadim Y. , Al-Ubaidi Muayyad R. , Naash Muna I. 
Published: July 2023

AbstractMutations in the photoreceptor-specific tetraspanin gene peripherin-2 (PRPH2) lead to widely varying forms of retinal degeneration ranging from retinitis pigmentosa to macular dystrophy. Both inter- and intra-familial phenotypic heterogeneity has led to much interest in uncovering the complex pathogenic mechanisms of PRPH2-associated disease. Majority of disease-causing mutations in PRPH2 reside in the second intradiscal loop, wherein seven cysteines control protein folding and oligomerization. Here, we utilize knockin models to evaluate the role of three D2 loop cysteine mutants (Y141C, C213Y and C150S), alone or in combination. We elucidated how these mutations affect PRPH2 properties, including oligomerization and subcellular localization, and contribute to disease processes. Results from our structural, functional and molecular studies revealed that, in contrast to our understanding from prior investigations, rods are highly affected by PRPH2 mutations interfering with oligomerization and not merely by the haploinsufficiency associated with these mutations. On the other hand, cones are less affected by the toxicity of the mutant protein and significantly reduced protein levels, suggesting that knockdown therapeutic strategies may sustain cone functionality for a longer period. This observation provides useful data to guide and simplify the current development of effective therapeutic approaches for PRPH2-associated diseases that combine knockdown with high levels of gene supplementation needed to generate prolonged rod improvement.
Protrudin-mediated ER-endosome contact sites promote phagocytosis
Authors: Elfmark Liv Anker , Wenzel Eva Maria , Wang Ling , Pedersen Nina Marie , Stenmark Harald , Raiborg Camilla 
Published: July 2023

AbstractDuring phagocytosis, endosomes both contribute with membrane to forming phagosomes and promote phagosome maturation. However, how these vesicles are delivered to the phagocytic cup and the phagosome has been unknown. Here, we show that Protrudin-mediated endoplasmic reticulum (ER)-endosome contact sites facilitate anterograde translocation of FYCO1 and VAMP7-positive late endosomes and lysosomes (LELys) to forming phagocytic cups in a retinal pigment epithelial-derived cell line (RPE1). Protrudin-dependent phagocytic cup formation required SYT7, which promotes fusion of LELys with the plasma membrane. RPE1 cells perform phagocytosis of dead cells (efferocytosis) that expose phosphatidylserine (PS) on their surface. Exogenous addition of apoptotic bodies increased the formation of phagocytic cups, which further increased when Protrudin was overexpressed. Overexpression of Protrudin also led to elevated uptake of silica beads coated with PS. Conversely, Protrudin depletion or abrogation of ER-endosome contact sites inhibited phagocytic cup formation resulting in reduced uptake of PS-coated beads. Thus, the Protrudin pathway delivers endosomes to facilitate formation of the phagocytic cup important for PS-dependent phagocytosis.
A fast, easy and reliable method for hamstrings graft size prediction in anterior cruciate ligament reconstruction
Authors: Minoli C. , Travi M. , Monti C. , Ferrua P. , Puce Marco , Radaelli S. , Menon A. , Tassi A. L. , Randelli P. S. 
Published: July 2023

AbstractPurposeThe aim of this study is to describe and validate a simple and reliable method to pre‐operatively predict the size of the ACL graft in the double strand technique with autologous semitendinosus–gracilis tendons on the same MRI used for ACL rupture diagnosis.MethodsThe study included 92 patients, with a median age of 31 years (IQR 26–41 years), 73/92 (79%) of whom were males. All patients that underwent an ACL reconstruction with doubled ST + GT between 2017 and 2022 were counted in the study.ResultsOverall, the median predicted graft diameter from MR imaging was similar to the actual graft diameter with no significant differences (n.s.). Regarding the comparison between predicted and actual graft size, concordance was 78/92 (85%, 95% CI 76–91%), with κ = 0.797 which corresponds to a level of agreement defined as “Strong”. Tendon sizes calculated on pre‐operative MRI were evaluated both with intra‐observer and inter‐observer reliability demonstrating a statistically reproducible method. The predicted graft was then compared to the reported one with a statistically significant reliability found.ConclusionThis study can help the surgeons to perform a fast pre‐operative planning of an ACL reconstruction for graft selection. If the planned graft with ST and GT is smaller than 8 mm, the clinician can decide to switch to a different type of graft or plan a different graft preparing technique and, therefore, reduce the risk of post‐operative ligament re‐rupture. The method proposed is reliable and reproducible. The major strength of the planning technique proposed is that it relies on data that are already available for the clinician before surgery, without the need of further analysis.Level of evidenceIV.
Amino acid analysis for peptide quantitation using reversed-phase liquid chromatography combined with multiple reaction monitoring mass spectrometry
Authors: Qasrawi Deema O. , Petrotchenko Evgeniy V. , Borchers Christoph H. 
Published: July 2023

AbstractAmino acid analysis (AAA) can be used for absolute quantitation of standard peptides after acid hydrolysis using 6 M HCl. Obtained individual amino acids can then be quantified by liquid chromatography-mass spectrometry (LC–MS). Achieving baseline separation of non-derivatized amino acids is challenging when reversed-phase (RP) chromatography is used. Several derivatization methods are commonly utilized to address this issue; however, derivatization has several drawbacks, such as derivative instability and lack of reproducibility. Currently, separation of non-derivatized amino acids is typically done using HILIC, but HILIC has problems of poor reproducibility and long column equilibration times. We developed a method to quantify non-derivatized amino acids, including methionine and cysteine, from peptide hydrolysates by RP-LC-MS without special pre-treatment of the samples. Samples were spiked with certified isotopically labeled (13C- and/or 15N-) amino acids as internal standards. The amino acids released from acid hydrolysis were then analyzed by RP-UPLC-MRM-MS and quantified using the analyte/internal standard chromatographic peak area ratios. Peptide quantitation was based on the sum of the individual amino acid concentrations from the known peptide sequences. The resulting method did not require derivatization, used standard C18-based reversed-phase liquid chromatography, did not require external calibration, was robust, and was able to quantify all 17 amino acids for which we had internal standards, including the sulfur-containing amino acids, cysteine and methionine, in their respective oxidized forms. This simple and robust method enabled the absolute quantitation of standard peptides using only acid hydrolysis and a standard RP-UPLC-MRM-MS setup.
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Postnatal imaging of conjoined twins: a customized multimodality approach
Authors: Mohammad Shaimaa Abdelsattar , AbouZeid Amr Abdelhamid , Ahmed Khaled A. , Abd-Elhamed Abeer Maghawry , Rawash Eldieb Leila M. 
Published: July 2023

AbstractMedical imaging plays a crucial role in the management of conjoined twins. The role of imaging is to explore the anatomy, outline the shared organs and determine whether surgery is feasible. It also serves as a roadmap for successful separation. Additionally, imaging helps with counseling parents about prognosis. This review aims to illustrate recent advances in different imaging modalities and their role in the management of the various types of conjoined twins with an emphasis on relevant tips for optimal imaging.
Graphical abstract