bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synaptotagmin-like protein 1 is a potential diagnostic and prognostic biomarker in endometrial cancer based on bioinformatics and experiments
Authors: Meijuan Cai , Meng Xu , Fang Liu , Qian Wang 
Published: January 2023

AbstractEndometrial cancer (EC) is one of the most common gynecologic malignancies. Identification of potential EC biomarkers is essential to improve the prognosis and development of therapies against EC. Synaptotagmin-like protein 1 (SYTL1), as a small GTPase Rab27 effector, mainly plays a role in vesicle trafficking and cytotoxic granule exocytosis in lymphocytes. However the role of SYTL1 in EC remains uncertain. We performed a comprehensive assessment of the relationship between SYTL1 and patient diagnosis and prognosis by analysis of EC patients’ data from TCGA. We employed the LinkedOmics and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database to analyze the biological function of SYTL1 in EC. In addition, the correlation between SYTL1 expression and its DNA methylation was performed by using cBioportal, UALCAN, TCGA Wanderer and MethSurv databases. We further assessed the link between SYTL1 and tumor-infiltrating immune cells by using gene set variation analysis (GSVA).Results We found that SYTL1 was highly expressed in EC patients and cell lines. And increased expression of SYTL1 was associated with age, clinical stage, histological type, histological grade and good overall survival (OS).SYTL1 DNA methylation is negatively associated with SYTL1 expression and UCEC patients’ OS. SYTL1 expression is closely correlated with immune infiltration. Furthermore, we carried out in vitro experiments to verify the results of bioinformatic analysis.Conclusion Our results demonstrated that the elevation of SYTL1 expression is associated with good OS and SYTL1 might be a potential diagnostic and prognostic marker in EC.
Telehealth multicomponent exercise and health education in breast cancer patients undergoing primary treatment: rationale and methodological protocol for a randomized clinical trial (ABRACE: Telehealth)
Authors: Henkin João S. , Botton Cíntia E. , Simon Mariana S. , Rocha Guilherme G. , Silveira Caroline B. , Gehrke Ricardo S. , Freitas Gabriella B. , Trajano Gabriel S. , Pinto Ronei S. , Pinto Stephanie S. 
Published: January 2023

AbstractBackgroundCurrent guidelines emphasize cancer patients should increase their physical activity levels, encouraging physical exercise practice as a complementary therapy to mitigate adverse effects during treatment. Telehealth can be a feasible method to improve adherence and interventional support for breast cancer patients, of which most do not meet sufficient physical activity levels after diagnosis. The Adaptations to Breast Cancer and Exercise Using Telehealth (ABRACE: Telehealth) study aims to investigate the effects of a 12-week telehealth multicomponent training program plus a health education program (MTHE), compared to a health education program alone (HE), on physical and psychological outcomes in breast cancer patients undergoing treatment.MethodsThis study is a randomized controlled trial. Women undergoing primary treatment (during or after chemotherapy) for breast cancer (stages I–III) will be randomly assigned to MTHE (twice a week) or HE (once a week). MTHE components are mobility, aerobic, balance, resistance, and flexibility home-based exercises, supervised by video call. The primary study outcome is cancer-related fatigue. The secondary outcomes are quality of life, symptoms of depression and anxiety, physical activity level, cancer-related cognitive impairment, and functional capacity. Other outcomes are adherence to interventions and a follow-up questionnaire evaluating the individual perception in motivation, lifestyle changes, and main barriers to participation. All outcomes will be remotely assessed before and after intervention. Our analysis will follow the intention-to-treat approach and per-protocol criteria, with additional sub-group analysis.DiscussionTo our knowledge, this is the first randomized clinical trial in breast cancer patients using a face-to-face videoconference strategy to supervise physical exercise. Our hypothesis is of superiority for the effects of MTHE on primary and secondary outcomes compared to the effects of only the health education intervention.Trial registrationAdaptations to Breast Cancer and Exercise Using Telehealth (ABRACE: Telehealth), NCT04641377. Registered on 23 November 2021,https://clinicaltrials.gov/ct2/show/NCT04641377
CANOPTIPHYS study protocol: Optimising PHYSical function before CANcer surgery: effects of pre-operative optimisation on complications and physical function after gastrointestinal cancer surgery in older people at risk—a multicentre, randomised, parallel-group study
Authors: Andersson Mikael , Egenvall Monika , Danielsson Johanna , Thorell Anders , Sturesson Christian , Soop Mattias , Nygren-Bonnier Malin , Rydwik Elisabeth 
Published: January 2023

Abstract
Background
This multicentre study explores the effects of pre-operative exercise on physical fitness, post-operative complications, recovery, and health-related quality of life in older individuals with low pre-operative physical capacity scheduled to undergo surgery for colorectal cancer. We hypothesise that this group of patients benefit from pre-operative exercise in terms of improved pre-operative physical function and lower rates of post-operative complications after surgery compared to usual care. Standardised cancer pathways in Sweden dictate a timeframe of 14–28 days from suspicion of cancer to surgery for colorectal cancer. Therefore, an exercise programme aimed to enhance physical function in the limited timeframe requires a high-intensity and high-frequency approach.

Methods
Participants will be included from four sites in Stockholm, Sweden. A total of 160 participants will be randomly assigned to intervention or control conditions. Simple randomisation (permuted block randomisation) is applied with a 1:1 allocation ratio. The intervention group will perform home-based exercises (inspiratory muscle training, aerobic exercises, and strength exercises) supervised by a physiotherapist (PT) for a minimum of 6 sessions in the pre-operative period, complemented with unsupervised exercise sessions in between PT visits. The control group will receive usual care with the addition of advice on health-enhancing physical activity. The physical activity behaviour in both groups will be monitored using an activity monitor. The primary outcomes are (1) change in physical performance (6-min walking distance) in the pre-operative period and (2) post-operative complications 30 days after surgery (based on Clavien-Dindo surgical score).

Discussion
If patients achieve functional benefits by exercise in the short period before surgery, this supports the implementation of exercise training as a clinical routine. If such benefits translate into lower complication rates and better post-operative recovery or health-related quality of life is not known but would further strengthen the case for pre-operative optimisation in colorectal cancer.

Trial registration
ClinicalTrials.gov NCT04878185. Registered on 7 May 2021. https://clinicaltrials.gov/ct2/home
Differential identification of Mannheimia haemolytica genotypes 1 and 2 using colorimetric loop-mediated isothermal amplification
Authors: Dassanayake Rohana P. , Clawson Michael L. , Tatum Fred M. , Briggs Robert E. , Kaplan Bryan S. , Casas Eduardo 
Published: January 2023

Abstract
Objective
Mannheimia haemolytica is the primary bacterial pathogen associated with bovine respiratory disease complex (BRDC). While M. haemolytica has been subdivided into 12 capsular serotypes (ST), ST1, ST2 and ST6 are commonly isolated from cattle. More recently, M. haemolytica strains isolated from North American cattle have been classified into genotypes 1 (ST2) and 2 (ST1 and ST6). Of the two genotypes, genotype 1 strains are frequently isolated from healthy animals whereas, genotype 2 strains are predominantly isolated from BRDC animals. However, isolation of both genotypes from pneumonic lung samples can complicate diagnosis. Therefore, the aim of this study was to develop a colorimetric loop-mediated isothermal amplification (LAMP) assay to differentiate M. haemolytica genotypes.

Results
The genotype specificity of the LAMP was tested using purified genomic DNA from 22 M. haemolytica strains (10 genotype 1, 12 genotype 2) and strains from four related Pasteurellaceae species; Bibersteinia trehalosi, Mannheimia glucosida, Pasteurella multocida, and Histophilus somni. Genotype 1 (adhesin pseudogene B1) specific-LAMP reactions amplified DNA only from genotype 1 strains while genotype 2 (adhesin G) reactions amplified DNA only from genotype 2 strains. The overall detection sensitivity and specificity of the newly developed colorimetric LAMP assay for each genotype were 100%. The limits of detection of two LAMP assays were 1–100 target gene copies per reaction. LAMP primers designed in this study may help the differential identification of M. haemolytica genotypes 1 and 2.
Linking structure and species richness to support forest biodiversity monitoring at large scales
Authors: Storch Felix , Boch Steffen , Gossner Martin M. , Feldhaar Heike , Ammer Christian , Schall Peter , Polle Andrea , Kroiher Franz , Müller Jörg , Bauhus Jürgen 
Published: January 2023

Abstract
Key message
Authors have analyzed the possible correlation between measurements/indicators of forest structure and species richness of many taxonomic or functional groups over three regions of Germany. Results show the potential to use structural attributes as a surrogate for species richness of most of the analyzed taxonomic and functional groups. This information can be transferred to large-scale forest inventories to support biodiversity monitoring.

Context
We are currently facing a dramatic loss in biodiversity worldwide and this initiated many monitoring programs aiming at documenting further trends. However, monitoring species diversity directly is very resource demanding, in particular in highly diverse forest ecosystems.

Aims
We investigated whether variables applied in an index of stand structural diversity, which was developed based on forest attributes assessed in the German National Forest Inventory, can be calibrated against richness of forest-dwelling species within a wide range of taxonomic and functional groups.

Methods
We used information on forest structure and species richness that has been comprehensively assessed on 150 forest plots of the German biodiversity exploratories project, comprising a large range of management intensities in three regions. We tested, whether the forest structure index calculated for these forest plots well correlate with the number of species across 29 taxonomic and functional groups, assuming that the structural attributes applied in the index represent their habitat requirements.

Results
The strength of correlations between the structural variables applied in the index and number of species within taxonomic or functional groups was highly variable. For some groups such as Aves, Formicidae or vascular plants, structural variables had a high explanatory power for species richness across forest types. Species richness in other taxonomic and functional groups (e.g., soil and root-associated fungi) was not explained by individual structural attributes of the index. Results indicate that some taxonomic and functional groups depend on a high structural diversity, whereas others seem to be insensitive to it or even prefer structurally poor stands.

Conclusion
Therefore, combinations of forest stands with different degrees of structural diversity most likely optimize taxonomic diversity at the landscape level. Our results can support biodiversity monitoring through quantification of forest structure in large-scale forest inventories. Changes in structural variables over inventory periods can indicate changes in habitat quality for individual taxonomic groups and thus points towards national forest inventories being an effective tool to detect unintended effects of changes in forest management on biodiversity.
Determinants of anemia among women of childbearing age: analysis of the 2018 Mali demographic and health survey
Authors: Armah-Ansah Ebenezer Kwesi 
Published: January 2023

AbstractBackgroundAnemia occurs at all stages of life and it is of public health concern as it serves as an indicator of quality nutrition and health of a society. Almost one third of the global prevalence of anemia occur among reproductive aged women and almost 40% of these women reside in sub-Saharan Africa including Mali. This study, therefore, sought to assess the determinants of anemia among women of childbearing age in Mali.MethodsSecondary data analysis of the 2018 Mali Demographic and health Survey (MDHS). Anemia in women was the outcome variable for the study. Data of 5,048 women aged 15–49 was used for the study. Using binary and multivariate logistic regression models, factors associated with anemia among women were identified. The analysis was conducted using Stata version 14.2 software and adjusted Odds Ratio (aOR) with a 95% Confidence Interval (CI) andp-value < 0.05 were used to see the significant association.ResultsThe prevalence of anemia among women of childbearing age in Mali is 63.5%. Of these, 4.3% and 24.9% were severely and mildly anemic respectively, and the rest 34.3% were moderately anemic. Women who had secondary education (aOR = 0.70, 95% CI: 0.58–0.84), overweight (aOR = 0.63, 95% CI: 0.50–0.81), exposure to mass media (aOR = 0.90, 95% CI = 0.76–1.49) and women with two births (aOR = 0.86, 95% CI = 0.71–1.05) were less likely to be anemic. Likely, richest wealth quintile (aOR = 0.73, 95% CI = 0.51–1.05), living in urban setting (aOR = 0.85, 95% CI = 0.70–1.03) and women in Kidal Region (aOR = 0.42, 95% CI = 0.27–0.65) were less likely to be anemic. However, pregnant women, women covered by health insurance, women with unimproved drinking water, women in communities with low literacy and low socioeconomic status had higher odds of anemia.ConclusionThese findings point to the need for community and household level public health sensitization interventions to highlight the pro-anemic factors and mitigating strategies. More especially, women with no education, pregnant women, women covered by health insurance, women from communities with low literacy and socioeconomic status ought to be the focus of such interventions.
Responding to incorrect ideas: science graduate teaching assistants’ operationalization of error framing and undergraduate students’ perception
Authors: Wan Tong , Doty Constance M. , Geraets Ashley A. , Saitta Erin K. H. , Chini Jacquelyn J. 
Published: January 2023

Abstract
Background
In college science laboratory and discussion sections, student-centered active learning strategies have been implemented to improve student learning outcomes and experiences. Research has shown that active learning activities can increase student anxiety if students fear that they could be negatively evaluated by their peers. Error framing (i.e., to frame errors as natural and beneficial to learning) is proposed in the literature as a pedagogical tool to reduce student anxiety. However, little research empirically explores how an instructor can operationalize error framing and how error framing is perceived by undergraduate students. To bridge the gap in the literature, we conducted a two-stage study that involved science graduate teaching assistants (GTAs) and undergraduate students. In stage one, we introduced cold calling (i.e., calling on non-volunteering students) and error framing to 12 chemistry and 11 physics GTAs. Cold calling can increase student participation but may increase student anxiety. Error framing has the potential to mitigate student anxiety when paired with cold calling. GTAs were then tasked to rehearse cold calling paired with error framing in a mixed-reality classroom simulator. We identified GTA statements that aligned with the definition of error framing. In stage two, we selected a few example GTA error framing statements and interviewed 13 undergraduate students about their perception of those statements.

Results
In the simulator, all the GTAs rehearsed cold calling multiple times while only a few GTAs made error framing statements. A thematic analysis of GTAs’ error framing statements identified ways of error indication (i.e., explicit and implicit) and framing (i.e., natural, beneficial, and positive acknowledgement). Undergraduate student interviews revealed specific framing and tone that are perceived as increasing or decreasing student comfort in participating in classroom discourse. Both undergraduate students and some GTAs expressed negative opinions toward responses that explicitly indicate student mistakes. Undergraduate students’ perspectives also suggest that error framing should be implemented differently depending on whether errors have already occurred.

Conclusion
Error framing is challenging for science GTAs to implement. GTAs’ operationalizations of error framing in the simulator and undergraduate students’ perceptions contribute to defining and operationalizing error framing for instructional practice. To increase undergraduate student comfort in science classroom discourse, GTAs can use implicit error indication. In response to students’ incorrect answers, GTAs can positively frame students’ specific ideas rather than discussing broadly how errors are natural or beneficial.
A critical evaluation of the content validity of patient-reported outcome measures assessing health-related quality of life in children with cancer: a systematic review
Authors: Rothmund Maria , Meryk Andreas , Rumpold Gerhard , Crazzolara Roman , Sodergren Samantha , Darlington Anne-Sophie , Riedl David ,   the EORTC Quality of Life Group
Published: January 2023

Abstract
Background
With increasing survival rates in pediatric oncology, the need to monitor health-related quality of life (HRQOL) is becoming even more important. However, available patient-reported outcome measures (PROMs) have been criticized. This review aims to systematically evaluate the content validity of PROMs for HRQOL in children with cancer.

Methods
In December 2021, a systematic literature search was conducted in PubMed. PROMs were included if they were used to assess HRQOL in children with cancer and had a lower age-limit between 8 and 12 years and an upper age-limit below 21 years. The COSMIN methodology for assessing the content validity of PROMs was applied to grade evidence for relevance, comprehensiveness, and comprehensibility based on quality ratings of development studies (i.e., studies related to concept elicitation and cognitive interviews for newly developed questionnaires) and content validity studies (i.e., qualitative studies in new samples to evaluate the content validity of existing questionnaires).

Results
Twelve PROMs were included. Due to insufficient patient involvement and/or poor reporting, the quality of most development studies was rated ‘doubtful’ or ‘inadequate’. Few content validity studies were available, and these were mostly ‘inadequate’. Following the COSMIN methodology, evidence for content validity was ‘low’ or ‘very low’ for almost all PROMs. Only the PROMIS Pediatric Profile had ‘moderate’ evidence. In general, the results indicated that the PROMs covered relevant issues, while results for comprehensiveness and comprehensibility were partly inconsistent or insufficient.

Discussion
Following the COSMIN methodology, there is scarce evidence for the content validity of available PROMs for HRQOL in children with cancer. Most instruments were developed before the publication of milestone guidelines and therefore were not able to fulfill all requirements. Efforts are needed to catch up with methodological progress made during the last decade. Further research should adhere to recent guidelines to develop new instruments and to strengthen the evidence for existing PROMs.
Echocardiography in acute stroke patients: a nationwide analysis in departments with certified stroke units in Germany
Authors: Rizos Timolaos , Jenetzky Ekkehart , Nabavi Darius Günther , Haeusler Karl Georg , Wachter Rolf , Ossenbrink Martin , Ringleb Peter Arthur , Busse Otto 
Published: January 2023

Abstract
Background
Echocardiography is highly relevant in patients with ischemic stroke or TIA. Utilization of routine echocardiographic examinations [transthoracic (TTE) or transesophageal (TEE)] on stroke units remains however unknown. To representatively examine echocardiographic rates on stroke units in Germany and to evaluate structural factors that may influence the decision to conduct echocardiography.

Methods
A nationwide analysis was performed by using certification audit data of all primary and comprehensive stroke centers (pSC and cSC) in Germany.

Results
Structural and organizational requirements of 310 departments (cSCs: 42.6%) were extracted. Median TTE rate was 63.3% (IQR 39.3–80.8), median TEE rate 21.3% (IQR 16.4–29.5). A cardiological department on site was present in 74.2%, and they were associated with higher TEE rates. TTE rates decreased with increasing numbers of patients (p = 0.026). Likewise, TEE rates decreased with increasing numbers of patients (p = 0.006), mediated by departments with cSCs (p = 0.008 for cSCs vs p = 0.230 for pSCs). TTE rates were far more inhomogeneously distributed than TEE rates and higher in pSCs (p = 0.011). Overall, 12.9% of centers did not perform any echocardiographic examination in at least 50% of all stroke patients.

Conclusion
More detailed recommendations regarding echocardiography should be included in future guidelines. Moreover, evaluating the impact of echocardiographic examinations on long-term prognosis in stroke patients should be focus of further evaluations.
Acquisition of consonant clusters and remediation of cluster reduction in Egyptian children
Authors: Saleh Marwa Mahmoud , El-Hamshary Ahmed Ashraf S. , Farag Eman Talaat Fekry , Boshnaq Maha Hussein 
Published: January 2023

Abstract
Background
Consonant clusters are a feature of many world languages. The acquisition of consonant clusters is one of the latest occurring aspects of speech acquisition in normally developing children. Difficulty producing consonant clusters has been found to contribute to high levels of unintelligibility in children with phonological impairment.
This is a cross-sectional descriptive study that was applied on 150 typically developing (TD) Egyptian Arabic children, with an age range of 30 to 48 months, using the Egyptian Monosyllabic Consonant Cluster Test (EMCCT). The children were divided into 3 groups, each group with an age range of 6 months. The objective was to determine the age of suppression of cluster reduction and to evaluate two different phonological therapies (minimal contrast and auditory bombardment) in children who have not acquired consonant clusters (less than 90% correct production).

Results
Age of suppression of cluster reduction in Egyptian children was found to be between 3 years 6 months and 4 years. There was a gradual decrease in frequency of occurrence of cluster reduction from 74% (2 years 6 months–3 years) to 46% (3 years–3 years 6 months) to 10% (3 years 6 months–4 years). There was significant statistical association between consonant clusters of obstruents + sonorants (stops + sonorants and fricatives + sonorants) between the different age groups (p value < 0.001). Both techniques of therapy showed significant improvement of post-therapy score. There was no significant difference between total intervention duration in both techniques; however, there was significant association between cumulative intervention intensity and minimal contrast therapy (p value < 0.001).

Conclusion
The Egyptian Monosyllabic Consonant Cluster Test (EMCCT) is a valid and reliable assessment tool for identification of percentage of occurrence of cluster reduction process. Both minimal contrast and auditory bombardment approaches are applicable for cluster reduction remediation.