bluuebunny/crossref_metadata_2025_split huggingface dataset:
Misspelled Logotypes: The Hidden Threat to Brand Identity
Authors: Rocabado Francisco , Perea Manuel , Duñabeitia Jon Andoni 
Published: May 2023

Abstract
Brand names are valuable company assets often accompanied by a unique graphical composition (i.e., as logotypes). Recent research has demonstrated that this uniqueness makes brand names and logotypes susceptible to counterfeiting through misspelling by transposition in tasks that require participants to identify correct spellings. However, our understanding of how brand names are incidentally processed when presented as logotypes is incomplete. To address this gap in knowledge, we conducted a virtual reality experiment to explore the transposed-letter confusability effect on brand name recognition. Participants were immersed in a virtual reality setting and incidentally exposed to logotypes that had correctly spelled brand names or included letter transpositions. Offline analyses revealed that participants were more accurate at recognizing brand names that had been presented with correct spellings than those that had been misspelled. Furthermore, participants exhibited false memory for misspelled logotypes, recalling them as if they had been spelled correctly. Thus, the present virtual reality experiment provides compelling evidence of the vulnerability of popular logotypes to counterfeiting via the misspelling of brand names.
Assessment of the integrated disease surveillance and response system implementation in North Kivu, Democratic Republic of the Congo, following a major Ebola outbreak, 2021
Authors: Kallay Ruth , Mbuyi Gisèle , Eggers Carrie , Coulibaly Soumaila , Kangoye David Tiga , Kubuya Janvier , Soke Gnakub Norbert , Mossoko Mathias , Kazambu Ditu , Magazani Alain , Luce Richard , Fonjungo Peter , Aruna Aaron 
Published: May 2023

Abstract
Background
The Democratic Republic of the Congo (DRC) experienced its largest Ebola Virus Disease Outbreak in 2018-2020. As a result of the outbreak, significant funding and international support were provided to Eastern DRC to improve disease surveillance. The Integrated Disease Surveillance and Response (IDSR) strategy has been used in the DRC as a framework to strengthen public health surveillance, and full implementation could be critical as the DRC continues to face threats of various epidemic-prone diseases. In 2021, the DRC initiated an IDSR assessment in North Kivu province to assess the capabilities of the public health system to detect and respond to new public health threats.
Methods
A descriptive cross-sectional survey was conducted using mixed quantitative and qualitative methods. Performance in IDSR core functions was assessed at multiple levels of the tiered health system through observations, key informant interviews, and analysis of health data. Qualitative data were also collected through focus groups and open-ended questions to guide the interpretation of the findings. Data were collected at the North Kivu provincial public health office, five health zones, 66 healthcare facilities, and from community health workers in 15 health areas.
Results
Data completeness was 100% at the provincial and health zone levels, and timeliness was 100% and 97% at the provincial and health zone levels, respectively. Healthcare facility data had an average completeness of 86%, and timeliness varied between health zones from 41 to 100%. The use of electronic Integrated Disease Surveillance and Response is not widely implemented. Data analyses are conducted mainly at the provincial and health zone levels, and are regularly disseminated to all levels. Preparedness capabilities were low, with 44% of health zones in North Kivu having a preparedness and response plan and 41% having a functional rapid response team.
Conclusion
Comparing the results of this assessment to the last IDSR assessment conducted in DRC in 2016, slight improvements in disease reporting have been made, despite several limitations in materials, equipment, infrastructure, and workforce training. This assessment highlights the need to move from outbreak-focused support and funding to building systems that can improve the long-term functionality of the routine disease surveillance system.
High-mannose glycosylation of ITGAM regulates the development and differentiation of trophoblast in the placenta
Authors: Cui Xinyuan , Wang Hao , Pei Xiaosong , Li Yaqi , Qin Huamin , Bi Jianlei , Liu Shuai , Yan Qiu 
Published: May 2023

Abstract
Background Development of placenta and differentiation of trophoblast is a hallmark event for successful pregnancy. Trophoblast stem (TS) cells proliferate and differentiate into TS-like cytotrophoblasts (CTBs), further formulate the differentiated subtypes, syncytiotrophoblasts (STBs) and invasive extravillous tropholoblasts (EVTs). Defective differentiation of TS-like CTBs is associated with severe gestational diseases. Protein glycosylation is an essential form of posttranslational modification. However, glycosylation-related mechanism controlling TS-like CTBs differentiation remains unknown. This study aims to investigate the function of high-mannose type glycans and ALG3 on TS-like CTBs differentiation. Furthermore, the mechanism of high-mannose and ITGAM during TS-like CTBs differentiation were explored.Methods Employing lectin microarray, the glycosylation expression traits were compared in the villi of miscarriage patients and healthy women. The expression of high-mannose and ALG3 were investigated by immunoblotting and immunofluorescence assays. The glycosylation proteins were screened by pull down and LC-MS/MS detection. Signaling pathway were screened by the human phosphokinase antibody array. The differentiation of TS-like CTBs were measured by immunoblotting and immunofluorescence assays.Results Lectin microarray results revealed that increased level of high-mannose type glycans on the TS-like CTBs of miscarriage patients compared with normal pregnancy women. Meantime, ALG3 levels increased in TS-like CTBs of miscarriage patients. Upregulating high-mannose type glycans by ALG3 hampered TS-like CTBs differentiated into STBs and EVTs, and arrested TS-like CTBs in the property stage. Furthermore, high level of high-mannose type glycans on ITGAM inhibited the binding of ITGAM and Fn, inactivating the p-STAT1 signaling pathway, further inhibiting TS-like CTBs differentiation potential.Conclusions These findings reveal that high-mannose type glycans, especially on ITGAM, hampered binding of ITGAM and Fn, which leads to the impaired TS-like CTBs differentiation by p-STAT1 signaling pathway. The present study provides novel insight into the function and mechanism of α1,3-linked high-mannose type glycans in TS-like CTBs differentiation in human placenta, which can also be used as a glycol molecular target for the treatment of miscarriage.
Rapid generation of a tomato male sterility system and its feasible application in hybrid seed production
Authors: Zhou Ming , Deng Lei , Yuan Guoliang , Zhao Wei , Ma Mingyang , Sun Chuanlong , Du Minmin , Li Chuanyou , Li Changbao 
Published: May 2023

Abstract
Male sterility enables reduced cost and high seed purity during hybrid seed production. However, progress toward its commercial applicationhas been slow in tomato due to the disadvantages of most natural male-sterile mutants. Here, we developed a practical method for efficient tomato hybrid seed production using a male sterile system with visible marker, which was rapidly generated by CRISPR/Cas9-mediated gene editing. Two closely linked genes, TM6 and DFR, which were reported to be candidates of ms15 (male sterile-15) and aw (anthocyanin without) locus respectively, were knocked out simultaneously in two elite tomato inbred lines. Mutagenesis of both genes generated green hypocotyl male-sterile (GHMS) lines, exhibiting male-sterility across different genetic backgrounds and environmental conditions and showing green hypocotyl due to defective anthocyanin accumulation, which serves as a reliable visible marker for selecting male-sterile plants at the seedling stage. We further proposed a strategy for multiplying the GHMS system and verified its high efficiency in stable male sterility propagation. Moreover, elite hybrid seeds were produced using GHMS system for potential side effects evaluation, and no adverse influences were found on seed yield, seed quality as well as important agronomic traits. This study provides a practical approach for the rapid generation and feasible application of male sterility in tomato hybrid breeding.
RILEM TC 266-MRP - Round-Robin Rheological Tests on High Performance Mortar and Concrete with Adapted Rheology: Evaluating Structural Build-up at Rest of Mortar and Concrete
Authors: Amziane Sofiane , Khayat Kamal , Sonebi Mohammed , Perrot Arnaud 
Published: May 2023

Abstract

This paper presents the results of a round-robin testing program undertaken by RILEM TC-266 - Measuring Rheological Properties of Cement-Based Materials in May 2018 at the Université d’Artois in Bethune France. Five types of rheometers were compared, they consisted of four ICAR rheometers, Viskomat XL rheometer, eBT-V rheometer, Sliding Pipe Rheometer (SLIPER), RheoCAD rheometer, and 4SCC rheometer, as well as the plate test. This paper discusses the results of the evolution of the static yield stress at rest of three mortar and five concrete mixtures that were determined using two ICAR rheometers, Viscomat XL and eBT-V rheometers, and the plate test. For the measurements carried out with rheometers, three different structural build-up indices (i.e., structural build-up rate, critical time, and coupled effects of initial static yield stress and rate of structural build-up) were determined. The indices were established using two static yield stress values measured after 10 and 40 minutes of rest as well as three static yield stress values that combine the two static yield stress measurements and the initial dynamic yield stresses obtained from the flow curves. The paper discusses the test results and highlights inaccuracies that could be encountered in determining the static yield stress. Test results indicate that the ICAR rheometers and the selected thixotropic indices can provide similar results, and that the spread of results obtained from different rheometers can be considerably reduced when using three yield stress values to calculate the rate of the static yield stress at rest. In order to enhance the accuracy of measurements, it is recommended to increase the number of measurements of the yield stress to at least three points over one hour after mixing to determine more accurately the various thixotropy indices.
Prevalence and factors associated with metabolic syndrome in patients with first hospitalization for major depression disorder：a large sample cross-sectional study
Authors: Tang Zhongyu , Zhang Lin , Liu Xuebing , Ma Jun 
Published: May 2023

Abstract
Metabolic syndrome (MetS) is a common comorbidity of major depressive disorder (MDD) that has serious adverse effects on physical health. The purpose of this study is to investigate the prevalence and factors influencing MetS in patients hospitalized for their first episode of MDD. The study included 981 patients who were admitted for MDD. Data on demographic and clinical characteristics were gathered, along with measurements of metabolism-related parameters and assessments of psychological and psychopathological symptoms. The prevalence of MetS in the study population was 9.68%. Patients with both MDD and MetS were found to have more extensive and significant demographic and clinical characteristics, higher levels of metabolism-related parameters, and more severe psychological and psychopathological symptoms. Risk factors for the diagnosis of MetS included the onset age of MDD, higher HAMD scores, and higher TSH levels. The risk factors for higher MetS scores included older age, being married, higher PSS scores, and higher levels of TSH and TC, while higher levels of LDL-C were protective. Our results suggest that MetS is not highly prevalent in patients with MDD, but certain risk factors may increase its likelihood and severity.
Targeting EIF5A improves liver fibrosis by inhibiting mitochondrial function of hepatic stellate cells
Authors: Di Yuzhu , Jiang Yanan , Zhi Fengnan , Hu Ying , Xu Ruiling , Liu Jingyang , Zhao Lei , Ning Dandan , Jin Shizhu 
Published: May 2023

Abstract
Background: Liver fibrosis (LF) is the pathological basis of a majority of chronic liver diseases, characterized by excessive activation of hepatic stellate cells (HSCs), inflammatory disorders and progressive scarring of the hepatic interstitial. Recently, sing-cell RNA sequencing (scRNA-seq) technology highlighted a much greater diversity between quiescent HSCs (qHSCs) and activated HSCs (aHSCs) in the progression of LF. Our study aims to investigate the role of Eif5a in liver fibrosis.
Methods: Bioinformatic analysis showed that the expression of Eif5a was associated with aHSCs. Immunofluorescence staining and qRT-PCR assays were performed to detected the expression and localization of Eif5a in both in vitro and in vivo hepatic fibrosis models. CCK-8, EdU, wound healing and flow cytometry assays verified the effect of si-EIF5A on aHSCs. H&E staining, Masson staining and immunohistochemistry assays detected the function of AAV-sh-Eif5a on mouse liver fibrosis. Further, transmission electron microscopy, mitochondrial membrane potential, Mito-Tracker Green, OCR and ATP experiments confirmed the relationship between EIF5A and mitochondrial biogenesis.
Results: Functionally, the silencing of Eif5a inhibited proliferation, migration, differentiation, and extracellular matrix deposition in aHSCs from both human and mouse origin. In vivo experiments, we found that silencing Eif5a slightly alleviated CCL4-induced liver fibrosis. Mechanistically, mitigating EIF5A triggered the mitochondrial morphology and function disorders to alleviated aHSCs activation.
Conclusions: In summary, our findings reveal that inhibition of EIF5A can alleviate LF through inhibiting mitochondrial function.
Cholestasis is associated with a higher rate of complications in both medical and surgical necrotizing enterocolitis.
Authors: Nayak Sujir Pritha , Huff Katie , Zaniletti Isabella , Ahmad Irfan , DiGeronimo ROBERT , Hair Amy , Kim Jae , Markel Troy , Piazza Anthony , Reber Kristina , Roberts Jessica , Sharma Jotishna , Sullivan Kevin , Premkumar Muralidhar , Yanowitz Toby 
Published: May 2023

Abstract
Objective: To evaluate the relationship between cholestasis and outcomes in medical and surgical necrotizing enterocolitis (NEC).
Study Design: A retrospective analysis of prospectively collected data from 1472 infants with NEC [455 medical (mNEC) and 1017 surgical (sNEC)] from the Children’s Hospital Neonatal Database.
Results: The prevalence of cholestasis was lower in mNEC versus sNEC (38.2% vs 70.1%, p<0.001). In both groups, cholestasis was associated with lower birth gestational age [mNEC: OR 0.79 (95% CI 0.68-0.92); sNEC: OR 0.86 (95%CI 0.79-0.95)] and increased days of parenteral nutrition [mNEC: OR 1.08 (95%CI 1.04-1.13); sNEC: OR 1.01 (95%CI 1.01-1.02)]. For both groups, the highest direct bilirubin was associated with the composite outcome mortality or length of stay >75th percentile [mNEC: OR 1.21 (95%CI 1.06-1.38); sNEC: OR 1.06 (95%CI 1.03-1.09)].
Conclusion: Cholestasis with both medical NEC and surgical NEC is associated with adverse patient outcomes including increased mortality or extreme length of stay.
Experiences of schooling in children with long-gap esophageal atresia in Sweden
Authors: Dellenmark-Blom Michaela , Reilly Colin , Öst Elin , Ax Sofie Örnö , Svensson Jan F , Kassa Ann-Marie , Jönsson Linus , Abrahamsson Kate , Gatzinsky Vladimir , Tollne AnnaMaria , Omling Erik , Stenström Pernilla , Lilja Helene Engstrand 
Published: May 2023

Abstract
Background: Children with long-gap esophageal atresia (LGEA) risk living with aerodigestive morbidity and mental health difficulties. No previous study has investigated their experiences of schooling, despite the importance of schools in children’s development, learning and social relationships. We aimed to describe experiences of schooling in children with LGEA in Sweden.
Method: Twenty-six children with LGEA aged 3-17 were recruited nationwide in Sweden. One parent completed a survey on their child’s school-based supports (according to definitions from the Swedish National Agency for Education), school absence, school satisfaction, school functioning (PedsQL 4.0), mental health (Strength and Difficulties Questionnaire) and current symptomatology. School data were compared to that from 95 children with EA who had primary anastomosis (PA), a hypothesized milder affected group. Mental health level was determined using validated norms; abnormal≥90 percentile. Data were analyzed using descriptives, correlation and Mann-Whitney-U test. Significance level was p<0.05.
Results: Seventeen children with LGEA (65.4%) had formal school-based support concerning nutritional intake (60%), educational needs (50%) and medical/special health needs (35%), which was significantly more frequent compared to children with PA overall (36.8%, p=0.013) and regarding nutritional intake support in school (20%, p< 0.001). In children with LGEA, school-based support was related to low birth weight (p=0.036), young child age (p=0.014), height ≤-2SD for age/sex (p=0.024) and an increased number of aerodigestive symptoms (p<0.05). All children with LGEA who had abnormal scores of mental health, except for one child, had school-based support. Nine children with LGEA (36%) had school absence ≥1times/month the past year, more frequently because of colds/airway infections (p=0.045) and gastro-intestinal related problems compared to PA (p=0.003). School functioning scores were not significantly different from children with PA (p=0.34) but correlated negatively with school-based support (<0.001) and school absence (p=0.002). One parent out of 26 reported their child’s school satisfaction as “not good”.
Conclusions: Children with LGEA commonly receive school-based support, reflecting multifaceted daily needs and disease severity. School absence is frequent and related to poorer school functioning. Collaborative strategies between specialized health care providers and schools are needed to accommodate for their disease-specific needs in school. Future research focusing on academic achievement in children with EA is needed.
Low energy consumption form of the U-shaped plan office building in the Yangtze River Delta
Authors: Ying Xiaoyu , Huangfu Fanyu , Tao Chi 
Published: May 2023

Abstract
The significance of form in green building design is well recognized, as it has a substantial impact on both energy performance and construction cost. This study investigates the impact of the U-shaped plan on the energy demand, which can be flexibly applied to irregular site forms and clustered building blocks, and is widely used in existing office buildings. Specifically, we select the U-shaped plan as the object of study and collect related case data primarily from Hangzhou and Shanghai. Using Python, we generate plans based on two lengths, two angles, and depth, which serve as basic parameters and boundary conditions. The EnergyPlus platform is utilized for dynamic simulation of the energy consumption of each model, and correlation analysis using the Spearman coefficient method identifies two planar factors (M1 and M2) that have the most significant influence on energy consumption, including five basic parameters. Regression fitting and regression evaluation of the model further identify morphological critical information in the plan. Our research reveals that the critical interval of M1 is in (0.4, 1.02), and the critical point of M2 is 109.38 m. Furthermore, the contour plot of M1-M2-E1 is obtained, illustrating the mutual trend between morphological parameters (M1 & M2) and annual energy use intensity, which serves as a useful aid for designing low energy consumption solutions.
NAc-DBS corrects depression-like behaviors in CUMS mouse model via disinhibition of DA neurons in the VTA
Authors: Song Nan , Gao Yan , Lu Shanshan , Yang Shenglian , Yuan Chao , Sun Wenyu 
Published: May 2023

Abstract
Major depressive disorder (MDD) is characterized by diverse debilitating symptoms that include loss of motivation and anhedonia. If multiple medications, psychotherapy, and electroconvulsive therapy fail in some patients with MDD, their condition is then termed treatment–resistant depression (TRD). MDD can be associated with abnormalities in the reward–system–dopaminergic mesolimbic pathway, in which the nucleus accumbens (NAc) and ventral tegmental area (VTA) play major roles. Deep brain stimulation (DBS) applied to the NAc alleviates the depressive symptoms of MDD. However, the mechanism underlying the effects of this DBS has remained elusive. In this study, using the chronic unpredictable mild stress (CUMS) mouse model, we investigated the behavioral and neurobiological effects of NAc–DBS on the multidimensional depression–like phenotypes induced by CUMS by integrating behavioral, in vivo microdialysis coupled with high-performance liquid chromatography–electrochemical detector (HPLC–ECD), calcium imaging, pharmacological, and genetic manipulation methods in freely moving mice. We found that long–term and repeated, but not single, NAc–DBS induced robust antidepressant responses in CUMS mice. Moreover, even a single trial NAc–DBS led to the elevation of the γ–aminobutyric acid (GABA) neurotransmitter, accompanied by the increase in dopamine (DA) neuron activity in the VTA. Both the inhibition of the GABAA receptor activity and knockdown of the GABAA–α1 gene in VTA–GABA neurons blocked the antidepressant effect of NAc–DBS in CUMS mice. Our results showed that NAc–DBS could disinhibit VTA–DA neurons by regulating the level of GABA and the activity of VTA–GABA in the VTA and could finally correct the depression–like behaviors in the CUMS mouse model.
Energy Efficient Mobile Adhoc Networks for LoRa for self-organizing network in Internet of Things
Authors: Narayandas Vikram , Maruthavanan Archana , Dugyala Raman 
Published: May 2023

Abstract
The Internet of Things is now a subject of study that is expanding quickly. Massive volumes of data management and collecting are addressed by web-connected sensors and devices. This sensor combination could be able to recognize and locate objects with more precision than a sensor working alone could. In any event, these dispersed sensors have to be able to operate in environments with little to no internet connectivity, which may greatly restrict their viability. These sensors might also be mounted on somewhat peculiar stages, further complicating connection and information flow. Using the energy-efficient B.A.T.M.A.N. (Better Approach To Mobile Ad hoc Networking) steering convention modified for LoRa, known as EC-BATMAN, a low-power long-range RF convention, to route sensor data through various hubs to arrive at web passages and allow these devices to connect with the cloud that would otherwise be unable is one potential solution. To improve the ability to anticipate route quality, several modifications to the algorithm will be examined, including the incorporation of additional sensors such to GPS and message signal solidarity. This framework seems to be a desirable, forgiving approach for this application.