bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population Genomics of Two Closely Related Anhydrobiotic Midges Reveals Differences in Adaptation to Extreme Desiccation
Authors: Shaikhutdinov Nurislam M , Klink Galya V , Garushyants Sofya K , Kozlova Olga S , Cherkasov Alexander V , Kikawada Takahiro , Okuda Takashi , Pemba Dylo , Shagimardanova Elena I , Penin Aleksey A , Deviatiiarov Ruslan M , Gazizova Guzel R , Cornette Richard , Gusev Oleg A , Bazykin Georgii A 
Published: September 2023

Abstract
The sleeping chironomid Polypedilum vanderplanki is capable of anhydrobiosis, a striking example of adaptation to extreme desiccation. Tolerance to complete desiccation in this species is associated with emergence of multiple paralogs of protective genes. One of the gene families highly expressed under anhydrobiosis and involved in this process is protein-L-isoaspartate (D-aspartate) O-methyltransferases (PIMTs). Recently, another closely related midge was discovered, Polypedilum pembai, which is able not only to tolerate desiccation but also to survive multiple desiccation–rehydration cycles. To investigate the evolution of anhydrobiosis in these species, we sequenced and assembled the genome of P. pembai and compared it with P. vanderplanki and also performed a population genomics analysis of several populations of P. vanderplanki and one population of P. pembai. We observe positive selection and radical changes in the genetic architecture of the PIMT locus between the two species, including its amplification in the P. pembai lineage. In particular, PIMT-4, the most highly expressed of these PIMTs, is present in six copies in the P. pembai; these copies differ in expression profiles, suggesting possible sub- or neofunctionalization. The nucleotide diversity of the genomic region carrying these new genes is decreased in P. pembai, but not in the orthologous region carrying the ancestral gene in P. vanderplanki, providing evidence for a selective sweep associated with postduplication adaptation in the former. Overall, our results suggest an extensive relatively recent and likely ongoing adaptation of the mechanisms of anhydrobiosis.
Prevalence and predictors of traditional medicine use among persons with diabetes in Africa: a systematic review
Authors: Ekpor Emmanuel , Osei Eric , Akyirem Samuel 
Published: September 2023

Abstract
The utilization of traditional medicine (TM) is prevalent among the general population in Africa; however, its use among individuals with diabetes in the region remains underdocumented. This review aimed to synthesize the available literature to identify the prevalence and predictors of TM use among persons with diabetes in Africa. A systematic search was conducted across multiple databases, including MEDLINE, Embase, CINAHL, and AMED, covering studies published from 2000 to April 2023. Of 1560 records identified, 24 articles met the inclusion criteria. The prevalence of TM use varied significantly, ranging from 12.4% to 77.1%, with a median prevalence of 50%. TM was commonly used concurrently with conventional medicine (CM) (35.4–88.4%), with a majority (63.8–91.3%) not disclosing TM use to healthcare providers. Female gender, long diabetes duration, use of oral antiglycaemic medication and family history of diabetes emerged as the most common factors that predicted the use of TM. This review highlights the widespread use of TM among individuals with diabetes in Africa, often in conjunction with CM. The high prevalence of undisclosed TM use emphasizes the urgent need for healthcare providers to actively inquire about TM use during clinical consultations to address potential herb–drug interactions and adverse effects.
Kinematic signatures of a low-mass planet with a moderately inclined orbit in a protoplanetary disk
Authors: Kanagawa Kazuhiro D , Ono Tomohiro , Momose Munetake 
Published: September 2023

Abstract
A planet embedded in a protoplanetary disk produces a gap by disk–planet interaction. It also generates velocity perturbation of gas, which can also be observed as deviations from the Keplerian rotation in the channel map of molecular line emission, called kinematic planetary features. These observed signatures provide clues to determine the mass of the planet. We investigated the features induced by a planet with an inclined orbit through three-dimensional hydrodynamic simulations. We found that a smaller planet, with an inclination of ∼10○–20○, can produce kinematic features as prominent as those induced by a massive coplanar planet. Despite the kinematic features being similar, the gap is shallower and narrower compared with the case in which the kinematic features are formed by a coplanar planet. We also found that the kinematic features induced by an inclined planet were fainter for rarer CO isotopologues because the velocity perturbation is weaker at the position closer to the midplane, which was different in the case with a coplanar massive planet. This dependence on the isotopologues is distinguished if the planet has an inclined orbit. We discussed two observed kinematic features in the disk of HD 163296. We concluded that the kink observed at 220 au can be induced by an inclined planet, while the kink at 67 au is consistent to that induced by a coplanar planet.
Nuclear factor OsNF-YC5 modulates rice seed germination by regulating synergistic hormone signaling
Authors: Jin Xinkai , Li Xingxing , Xie Zizhao , Sun Ying , Jin Liang , Hu Tingzhang , Huang Junli 
Published: September 2023

Abstract
Regulation of seed dormancy/germination is of great importance for seedling establishment and crop production. Nuclear factor-Y (NF-Y) transcription factors regulate plant growth and development, as well as stress responses; however, their roles in seed germination remain largely unknown. In this study, we reported that NF-Y gene OsNF-YC5 knockout increased, while its overexpression reduced, the seed germination in rice (Oryza sativa L.). ABA-induced seed germination inhibition assays showed that the osnf-yc5 mutant was less sensitive but OsNF-YC5-overexpressing lines were more sensitive to exogenous ABA than the wild type. Meanwhile, MeJA treatment substantially enhanced the ABA sensitivity of OsNF-YC5-overexpressing lines during seed germination. Mechanistic investigations revealed that the interaction of OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE 9 (SAPK9) with OsNF-YC5 enhanced the stability of OsNF-YC5 by protein phosphorylation, while the interaction between JASMONATE ZIM-domain protein 9 (OsJAZ9) and OsNF-YC5 repressed OsNF-YC5 transcriptional activity and promoted its degradation. Furthermore, OsNF-YC5 transcriptionally activated ABA catabolic gene OsABA8ox3, reducing ABA levels in germinating seeds. However, the transcriptional regulation of OsABA8ox3 by OsNF-YC5 was repressed by addition of OsJAZ9. Notably, OsNF-YC5 improved seed germination under salinity conditions. Further investigation showed that OsNF-YC5 activated the high-affinity K+ transporter gene (OsHAK21) expression, and addition of SAPK9 could increase the transcriptional regulation of OsHAK21 by OsNF-YC5, thus substantially reducing the ROS levels to enhance seed germination under salt stress. Our findings establish that OsNF-YC5 integrates ABA and JA signaling during rice seed germination, shedding light on the molecular networks of ABA-JA synergistic interaction.
Single-cell RNA-sequencing profiles reveal the developmental landscape of theManihot esculentaCrantz leaves
Authors: Zang Yuwei , Pei Yechun , Cong Xinli , Ran Fangfang , Liu Liangwang , Wang Changyi , Wang Dayong , Min Yi 
Published: September 2023

AbstractCassava (Manihot esculenta Crantz) is an important crop with a high photosynthetic rate and high yield. It is classified as a C3–C4 plant based on its photosynthetic and structural characteristics. To investigate the structural and photosynthetic characteristics of cassava leaves at the cellular level, we created a single-cell transcriptome atlas of cassava leaves. A total of 11,177 high-quality leaf cells were divided into 15 cell clusters. Based on leaf cell marker genes, we identified 3 major tissues of cassava leaves, which were mesophyll, epidermis, and vascular tissue, and analyzed their distinctive properties and metabolic activity. To supplement the genes for identifying the types of leaf cells, we screened 120 candidate marker genes. We constructed a leaf cell development trajectory map and discovered 6 genes related to cell differentiation fate. The structural and photosynthetic properties of cassava leaves analyzed at the single cellular level provide a theoretical foundation for further enhancing cassava yield and nutrition.
Plasticity of wood and leaf traits related to hydraulic efficiency and safety is linked to evaporative demand and not soil moisture in rubber (Hevea brasiliensis)
Authors: Waite Pierre-André , Leuschner Christoph , Delzon Sylvain , Triadiati Triadiati , Saad Asmadi , Schuldt Bernhard 
Published: September 2023

Abstract
The predicted increase of drought intensity in South-East Asia has raised concern about the sustainability of rubber (Hevea brasiliensis Müll. Arg.) cultivation. In order to quantify the degree of phenotypic plasticity in this important tree crop species, we analysed a set of wood and leaf traits related to the hydraulic safety and efficiency in PB260 clones from eight small-holder plantations in Jambi province, Indonesia, representing a gradient in local microclimatic and edaphic conditions. Across plots, branch embolism resistance (P50) ranged from −2.14 to −2.58 MPa. The P50 and P88 values declined, and the hydraulic safety margin increased, with an increase in the mean annual vapour pressure deficit (VPD). Among leaf traits, only the changes in specific leaf area were related to the differences in evaporative demand. These variations of hydraulic trait values were not related to soil moisture levels. We did not find a trade-off between hydraulic safety and efficiency, but vessel density (VD) emerged as a major trait associated with both safety and efficiency. The VD, and not vessel diameter, was closely related to P50 and P88 as well as to specific hydraulic conductivity, the lumen-to-sapwood area ratio and the vessel grouping index. In conclusion, our results demonstrate some degree of phenotypic plasticity in wood traits related to hydraulic safety in this tropical tree species, but this is only in response to the local changes in evaporative demand and not soil moisture. Given that VPD may increasingly limit plant growth in a warmer world, our results provide evidence of hydraulic trait changes in response to a rising evaporative demand.
Cerebral Vasospasm After Subarachnoid Hemorrhage: Respective Short-Term Effects of Induced Arterial Hypertension and its Combination With IV Milrinone: A Proof-of-Concept Study Using Transcranial Doppler Ultrasound
Authors: Lakhal Karim , Fresco Marion H. , Hivert Antoine , Rozec Bertrand , Cadiet Julien 
Published: September 2023

OBJECTIVES:
It is unclear whether IV milrinone relaxes spasmed cerebral arteries and therefore reduces cerebral blood mean velocity (Vmean). In patients treated for cerebral vasospasm, we aimed to assess and delineate the respective impacts of induced hypertension and its combination with IV milrinone on cerebral hemodynamics as assessed with transcranial Doppler.

DESIGN:
Observational proof-of-concept prospective study.

SETTING:
ICU in a French tertiary care center.

PATIENTS:
Patients with aneurysmal subarachnoid hemorrhage who received induced hypertension (mean arterial blood pressure [MBP] of 100–120 mm Hg) and IV milrinone (0.5 µg/kg/min) for moderate-to-severe cerebral vasospasm. We excluded patients who underwent invasive angioplasty or milrinone discontinuation within 12 hours after the diagnosis of vasospasm.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
Vmean was measured at vasospasm diagnosis (TDIAGNOSIS), after the induction of hypertension (THTN), and 1 (THTN+MILRINONE_H1) and 12 hours after the adjunction of IV milrinone (THTN+MILRINONE_H12). Thirteen patients were included. Median Vmean was significantly lower (p < 0.01) at THTN+MILRINONE_H1 (99 [interquartile range (IQR) 89; 134] cm.s−1) and THTN+MILRINONE_H12 (85 [IQR 73–127] cm/s) than at TDIAGNOSIS (136 [IQR 115–164] cm/s) and THTN (148 [IQR 115–183] cm/s), whereas TDIAGNOSIS and THTN did not significantly differ. In all patients but one, Vmean at THTN+MILRINONE_H1 was lower than its value at TDIAGNOSIS (p = 0.0005). Vmean-to-MBP and Vmean-to-cardiac output (CO) ratios (an assessment of Vmean regardless of the level of MBP [n = 13] or CO [n = 7], respectively) were, respectively, similar at TDIAGNOSIS and THTN but were significantly lower after the adjunction of milrinone (p < 0.01).

CONCLUSIONS:
The induction of arterial hypertension was not associated with a significant decrease in Vmean, whereas the adjunction of IV milrinone was, regardless of the level of MBP or CO. This suggests that IV milrinone may succeed in relaxing spasmed arteries.
The role of the intestine in metabolic dysregulation in murine Wilson disease
Authors: Sarode Gaurav V. , Mazi Tagreed A. , Neier Kari , Shibata Noreene M. , Jospin Guillaume , Harder Nathaniel H.O. , Caceres Amanda , Heffern Marie C. , Sharma Ashok K. , More Shyam K. , Dave Maneesh , Schroeder Shannon M. , Wang Li , LaSalle Janine M. , Lutsenko Svetlana , Medici Valentina 
Published: September 2023

Background:
The clinical manifestations of Wilson disease (WD) are related to copper accumulation in the liver and the brain, but little is known about other tissue involvement regarding metabolic changes in WD. In vitro studies suggested that the loss of intestinal ATP7B affects metabolic dysregulation in WD. We tested this hypothesis by evaluating the gut microbiota and lipidome in 2 mouse models of WD and by characterizing a new mouse model with a targeted deletion of Atp7b in the intestine.

Methods:
Cecal content 16S sequencing and untargeted hepatic and plasma lipidome analyses in the Jackson Laboratory toxic-milk and the Atp7b null global knockout mouse models of WD were profiled and integrated. Intestine-specific Atp7b knockout mice (Atp7b
ΔIEC) were generated and characterized using targeted lipidome analysis following a high-fat diet challenge.

Results:
Gut microbiota diversity was reduced in animal models of WD. Comparative prediction analysis revealed amino acid, carbohydrate, and lipid metabolism functions to be dysregulated in the WD gut microbial metagenome. Liver and plasma lipidomic profiles showed dysregulated triglyceride and diglyceride, phospholipid, and sphingolipid metabolism in WD models. However, Atp7b
ΔIEC mice did not show gut microbiome differences compared to wild type. When challenged with a high-fat diet, Atp7b
ΔIEC mice exhibited profound alterations to fatty acid desaturation and sphingolipid metabolism pathways as well as altered APOB48 distribution in intestinal epithelial cells.

Conclusions:
Gut microbiome and lipidome underlie systemic metabolic manifestations in murine WD. Intestine-specific ATP7B deficiency affected both intestinal and systemic response to a high-fat challenge but not the microbiome profile, at least at early stages. WD is a systemic disease in which intestinal-specific ATP7B loss and diet influence the phenotype and the lipidome profile.
The empirical dietary inflammatory pattern score and the risk of nonalcoholic fatty liver disease and cirrhosis
Authors: Ibrahim Maryam K. , Wilechansky Robert M. , Challa Prasanna K. , Zhang Xuehong , Giovannucci Edward , Stampfer Meir , Chan Andrew T. , Simon Tracey G. 
Published: September 2023

Background:
Diet plays an important role in the pathogenesis of NAFLD. Inflammation is a potential mechanism linking diet to NAFLD development and its progression to cirrhosis.
1
We analyzed data from a large, prospective cohort of US women to examine the influence of dietary inflammatory potential on the long-term risk of developing NAFLD and cirrhosis.

Methods:
We prospectively followed 96,016 women in the Nurses’ Health Study II cohort (1995–2017) who were free of chronic liver disease, including NAFLD, at baseline. The inflammatory potential of the diet was ascertained using an established, food-based empirical dietary inflammatory pattern score. Cox proportional hazard models were used to estimate multivariable-adjusted hazard ratios and 95% CIs for incident NAFLD and cirrhosis.

Results:
Over 2,085,947 person-years of follow-up, we documented 4389 cases of incident NAFLD and 102 cases of incident cirrhosis. Increasing cumulative average empirical dietary inflammatory pattern (EDIP) score was significantly and positively associated with incident NAFLD (multivariable-adjusted HR 1.31 per each 1-U increase in EDIP score, p-trend < 0.0001) and cirrhosis (p-trend of 0.034). Our findings also were consistent when examining recent diets using simple updated EDIP scores. In analyses of specific EDIP components, we observed an increased risk of incident NAFLD and cirrhosis with higher consumption of certain proinflammatory components of the EDIP score.

Conclusions:
Dietary patterns with a higher proinflammatory potential may be associated with a higher risk of developing both NAFLD and cirrhosis. Reducing the inflammatory potential of diet may potentially provide an effective strategy for preventing the development of NAFLD and progression to cirrhosis.
Left ventricular hypertrophy and mortality in ethnic minority groups in the UK: e-ECHOES study
Authors: Shantsila Eduard , Shantsila Alena , Williams Nefyn , Lip Gregory Y.H. , Gill Paramjit S. 
Published: September 2023

Objectives:Hypertension is the key modifiable cardiovascular risk factor but is underdiagnosed, and its scale in South Asian and African-Caribbean communities is unknown. Left ventricular hypertrophy (LVH) is a measure of target organ damage in uncontrolled hypertension. The study assesses LVH prevalence in South Asian and African-Caribbean communities and its impact on mortality.Method:This study is based on the large prospective UK community Ethnic-Echocardiographic Heart of England Screening Study (E-ECHOES, age ≥45 years). Left ventricular mass index (LVMI) was calculated using echocardiography to establish LVH. The predictive value of LVH all-cause and cardiovascular mortality was assessed using Cox regression.Results:The study included 3200 South Asians (age 59 ± 10 years, 52% women, 45% had a history of hypertension, 5.8 ± 1.0-year follow-up). LVH was found in 1568 (49%), of whom 45% did not have hypertension diagnosis. On Cox regression, LVH was independently associated with all-cause mortality [hazard ratio 1.38, 95% confidence interval (95% CI) 1.01–1.88], cardiovascular mortality (hazard ratio 2.64, 95% CI 1.21–3.73). The projected overall hypertension prevalence was 82%, undiagnosed hypertension prevalence 37%. The study included 1858 African-Caribbeans (age 62 ± 12, 45% women, 45% had history of hypertension, 5.1 ± 0.9-year follow-up). LVH was found in 1186 (64%), of whom 32% did not have hypertension diagnosis. LVH was borderline associated with all-cause mortality (hazard ratio 1.57, 95% CI 1.01–2.44), but not cardiovascular mortality (hazard ratio 1.82, 95% CI 0.80–4.16). The projected overall hypertension prevalence was 78.5%, and undiagnosed hypertension prevalence was 20.8%.Conclusion:UK South Asians and African-Caribbeans have a high prevalence of hypertension, which is often underdiagnosed and poorly controlled.
PARADOXICAL EXACERBATION OF SCLEROUVEITIS AFTER RITUXIMAB INFUSION
Authors: Vergouwen Daphne P. C. , Hermans Maud A. W. , Sels Lise 
Published: September 2023

Purpose:
To report on an unexpected side effect of rituximab, a B-cell–targeting monoclonal agent, in a patient with severe sclerouveitis, leading to severe visual loss.

Methods:
Observational case report.

Results:
A 61-year-old female patient was treated with rituximab for a severe sclerouveitis occurring with granulomatosis with polyangiitis. Initially, sclerouveitis responded partly to a high dose of oral corticosteroids. However, her sclerouveitis recurred after 2 months during tapering below 20 mg daily. The days following the rituximab infusions, aggravation of the intraocular inflammation was seen, endangering visual acuity. No evidence for an infection or other cause was found. High doses of oral corticosteroids were started within one week, whereafter the ocular inflammation resolved. For persisting hypotony and chorioretinal detachment, a combined pars plana vitrectomy with phacoemulsification and silicone oil tamponade was performed. The retina remained attached under oil tamponade with partial improvement of the best-corrected visual acuity to finger counting.

Conclusion:
Ophthalmologist should be aware of the possibility of this paradoxical local reaction to rituximab infusion, particularly in bilateral and/or severe cases, which carry a risk of a poor outcome.
Severe Lupus Nephritis with Thrombotic Microangiopathy: A Case Report
Authors: Hu Xiaoxuan , Qiu Jiamei , Su Guobin , Hou Haijing 
Published: September 2023

ABSTRACTThrombotic microangiopathy (TMA), a vascular lesion in lupus nephritis (LN), is rare but can be life-threatening. Early recognition and prompt treatment have significant impacts on clinical outcomes. A 28-year-old female with clinical manifestations of thrombocytopenia, severe anemia, and renal failure was diagnosed with systemic lupus erythematosus. However, the platelet count declined continuously, and hemolysis persisted even after pulse infusion of glucocorticoid. A disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13 (ADAMTS13) activity test (50.3%) and kidney biopsies confirmed the diagnosis of LN combined with TMA. After timely plasmapheresis, the serum creatinine level returned to normal and stabilized over the follow-up of six years. Patients with LN presenting with a continuous decline in platelet count, even after intensive glucocorticoid and immunosuppressant therapy, should pay attention to the potential occurrence of TMA. Timely ADAMTS13 activity testing and kidney biopsies assist in early diagnosis, and plasmapheresis may improve patient outcomes.
Blood Flow Restriction Training in Patients With Rotator Cuff Tendinopathy: A Randomized, Assessor-Blinded, Controlled Trial
Authors: Kara Dilara , Ozcakar Levent , Demirci Serdar , Huri Gazi , Duzgun Irem 
Published: September 2023

Objectives:
To investigate the effects of low-load blood flow restriction (BFR) training on shoulder muscle thickness, rotator cuff (RC) strength, and shoulder symptoms in patients with RC tendinopathy.

Design:
A randomized, assessor-blinded, controlled trial.

Settings:
Physiotherapy clinic at a university.

Participants:
Twenty-eight patients were randomized into an 8-week (2 times/week) shoulder rehabilitation, that is, BFR or non-BFR group.

Interventions:
BFR training.

Main outcome measures:
(1) RC, deltoid, scapula retractor, and biceps muscle thicknesses and shoulder internal rotation (IR) and external rotation (ER) strengths. (2) Shoulder pain/function.

Results:
The BFR group had a greater increase in biceps muscle thickness (P = 0.002) and shoulder IR strength at 60 degrees/s (P = 0.040) than the non-BFR group. No differences between the 2 groups were observed in other measurements. Significant improvements in supraspinatus, infraspinatus, and scapula retractor muscle thicknesses and in shoulder ER and IR strengths were observed over time in both the groups (all P < 0 .05). Also, shoulder pain decreased and shoulder function increased over time in both the groups (all P < 0 .05).

Conclusions:
Low-load BFR training resulted in a greater increase in biceps thickness and shoulder IR strength compared with the non-BFR group in patients with RC tendinopathy. However, there was no superiority of either exercise training regarding the RC, scapula retractor, deltoid muscle thicknesses, or improvements in shoulder ER strength and shoulder pain/function.

Clinical Trial Registry Name and Registration Number:
The study was registered in ClinicalTrials.gov named Blood Flow Restriction Training in Patients with Shoulder Pain and the registration number is NCT04333784.