bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compatibility of Calycosin-Tanshinone IIA improves Ang II-induced renal artery endothelial cell dysfunction through lncRNA-mRNA coexpression network
Authors: YanYun Jiang , Cong Han , WanLi Xu , YuQiu Li , Liu Yao 
Published: August 2023

Abstract
Background
Long noncoding RNAs (lncRNAs) have been found to have a significant impact on the development of endothelial dysfunction. Nevertheless, the precise mechanism underlying the compatibility of Calycosin-Tanshinone IIA in mitigating the dysfunction of rat renal artery endothelial cells (RRAECs) through the coexpression network of lncRNA-mRNA remains uncertain.
Methods
In the present investigation, an experimental model of endothelial cell injury was established by subjecting RRAECs to Ang II (5×10− 7mol/L) for a duration of 24 h. Subsequently, this model was treated with a combination of Calycosin (3mg/L) and Tanshinone Ⅱ (3mg/L). The changes in total ATP levels and autophagy function in RRAECs were evaluated using the ATP assay and dansylcadaverine (MDC) staining, respectively. Annexin V-FITC/PI staining and transwell assay were utilized to quantify the apoptosis rate and migration function of RRAECs. Moreover, the utilization of RNA-sequencing technology facilitated the identification of differentially expressed (DE) lncRNAs and mRNAs between various groups. Subsequently, a coexpression network between DE lncRNAs and mRNAs was constructed, followed by the implementation of GO and KEGG pathway enrichment analyses to elucidate the functional implications of the DE mRNAs interacting with lncRNAs within this network.
Results
The compatibility of Calycosin and Tanshinone IIA had the ability to activate autophagy, decrease apoptosis rate, enhance total ATP levels, and facilitate migration of RRAECs induced by Ang II. The sequencing data demonstrated that the compatibility of Calycosin and Tanshinone IIA reversed the disordered expression of 146 DE lncRNAs and 43 DE mRNAs in Ang Ⅱ-induced RRAECs. Furthermore, a lncRNA-mRNA coexpression network consisting of 28 DE lncRNAs and 7 DE mRNAs was established. GO enrichment analyses unveiled that the DE mRNAs that interacted with lncRNAs within this network were involved in the regulation of ATPase activity, arachidonic acid metabolic process, triglyceride metabolic process. Moreover, the KEGG pathways demonstrated a significant enrichment of the MAPK signaling pathway.
Conclusions
The potential of Calycosin and Tanshinone IIA compatibility to mitigate the dysfunction of RRAECs induced by Ang II, possibly through the involvement of the lncRNA-mRNA coexpression network, suggested a promising avenue for intervention in delaying the progression of hypertensive renal damage.
Academic Transition from High School to an Undergraduate Medical Program Using Active Methodologies Within an Integrated Curriculum One Qualitative Research
Authors: Silva Alexandre , Quaresma Juarez , Silva Enzo 
Published: August 2023

Abstract
Introduction: When transitioning from high school to an undergraduate medical program that uses active teaching methodologies, students often encounter clear obstacles in their first semester, such as breaking down disciplinary barriers, adapting to the dynamics of different curricular components, adjusting to an evaluation process that is different from what they are used to, as well as a lack of understanding of the program’s pedagogical proposal.
Objective: This study aims to understand the challenges faced by medical undergraduate students in their first semester, specifically in relation to the use of active teaching methodologies within an integrated curriculum.
Method: A qualitative study was conducted through interviews with 15 students from a private medical school who started in the first semester of 2018. The data was categorized and subjected to discourse analysis.
Results: The interviewees reported difficulties in understanding the educational proposal. Although they viewed the experience of adapting to the course's methodology positively, they found it challenging. They reported that there was insufficient explanation about the evaluation processes and proposed that clearer educational objectives be established for the students.
Conclusion: The need for a more comprehensive explanation of the pedagogical project and its teaching strategies, the importance of valuing practical training in health services, and the clarification of evaluation processes and educational objectives were highlighted by the interviewed students as key pillars for a successful transition from high school to medical education.
The Dynamic Nexus: Exploring the Interplay of BMI before, during, and after Pregnancy with Metabolic Syndrome (MetS) Risk in Chinese Lactating Women
Authors: Chen Liangxia , Ma Jie , Su Guanmin , Yin Longlong , Jiang Xiuyu , Wang Xiangxiang , Liu Lele , Zhang Xiaofei , Xu Xiaohui , Li Suyun , Zhang Gaohui , Zhao Ran , Yu Lianlong 
Published: August 2023

Abstract
Background and aim: The health implications of BMI and MetS in lactating women are significant. This study aims to investigate the relationship between risk of Mets in lactation and BMI in four stages: pre-pregnancy, prenatal period, 42 days postpartum, and current lactation.
Methods and results: A total of 1870 Lactating Women within 2 years after delivery were included from "China Child and Lactating Mother Nutrition Health Surveillance (2016-2017)". Logistic regression model and Restricted cubic spline (RCS) were used to estimate the relationship between BMI and risk of MetS. ROC analysis was used to determine the threshold for the risk of MetS. Chain mediating effect analysis was used to verify the mediating effect. BMI of MetS group in all stages were higher than non-MetS group (P<0.0001). There were significant positive correlations between BMI in each stage and ORs of MetS during lactation (P<0.05). The best cut-off values for BMI in the four stages were 23.47, 30.49, 26.04 and 25.47kg/m2. The non-linear spline test at BMI in 42 days postpartum, current and MetS in lactation was statistically significant (P non-linear=0.0223, 0.0003). The mediation effect of all chains have to work through lactation BMI. The total indirect effect accounted for 80.95% of the total effect.
Conclusions: The risk of MetS in lactating women is due to a high BMI base before pregnancy and postpartum. High BMI in all stages of pregnancy and postpartum were risk factors for MetS in lactation. BMI during lactation plays a key role in the risk of MetS.
High Levels of Preoperative Albumin and Albumin-globulin Ratio associated with the Higher Survival Rate in Gastric Cancer Patients
Authors: Ye Lei , Qi Qinghua , Xiao Sha , Petersen Jindong Ding , Cao Wenting 
Published: August 2023

Abstract
Purpose: The preoperative albumin-globulin ratio (AGR) is an inflammation-associated factor related to the overall survival in various malignancies. The objective of this study was to investigate preoperative serum AGR, albumin, and globulin level for the survival rate of GC.
Methods: This study was a retrospective cohort study comprising 771 GC patients who underwent the first-time gastrectomy between 1st January 2014 to 31st December 2018 at the Second People’s Hospital of Wuhu City, Anhui Province, China. For the eligible patients, we followed them since the first day of the operation to death, immigrate, or 31st of May 2021 (end of the study), whichever came first. Serum AGR and proteins were grouped according to quartiles (Q1 as the reference group). Cox proportional hazards regression models were applied for the analysis.
Results: Of 771 GC patients, 322 (41.8%) died during a median 41.00 moths of follow-up. Multivariate Cox analysis adjusting for confounders showed that, preoperative AGR level were significantly associated with GC mortality risk, i.e., following by the serum levels increased, the survival rate increased (Q2: HR =0.727, Q3: HR = 0.676, Q4: HR = 0.656, p for trend = 0.007). Albumin level showed a similar protective pattern but not globulin. Stratification analysis showed among the patients who were advance age, higher weight, advanced pTNM stage, and M0 group, higher preoperative AGR level, higher survival rate (p < 0.05).
Conclusion: These results showed that higher level of preoperative AGR and albumin were protective against risk of death in GC patients with first gastrectomy
Energy Efficiency under Mobility Control for Device-to-Device in Downlink Multi-cell OFDMA Systems with Fractional Frequency Reuse and Cooperative Relaying
Authors: Najjar Abdelhalim 
Published: August 2023

Abstract
This paper addresses the mobility control (MC) for Device to Device (D2D) in downlink multi-cell OFDMA system with cooperative relaying and Fractional Frequency Reuse (FFR) scheme. D2D equipments are characterized by limited capacity of battery and suffer from Co-Channel Interference (CCI) at cell edge. FFR and cooperative relaying schemes are proposed to improve energy efficiency (EE) and avoid CCI. In the edge region, according to a difference set and using the sectorization technique, the FFR of 7/3 and 7/4 have been applied. The deployment of FFR with Amplify and Forward (AF) fixed relays improve significantly the system performance of Frequency Reuse Factor (FRF) of 1 and 3.
Curvature correction factors for the independent verification of dose monitor units of electron treatment plans calculated in Eclipse.
Authors: Slama Luke , Mahmood Talat , Mckernan Brendan 
Published: August 2023

Abstract
Electron Monte Carlo (MC) dose calculation algorithms are sensitive to the incidence angle and curvature of the body contour of the patient. Over 10% difference between Treatment Planning System (TPS) and Independent Monitor Unit (IMU) calculations have been reported in the literature. We also observed similar results while using ClearCalc IMU calculation for electrons treatments planned in the Eclipse TPS. These plans can have over 10% difference to ClearCalc calculations when there is significant curvature of the body contour on the incident beam. The aim of this project is to measure and apply curvature correction factors (CCFs) in the clinical setting. Spherical and cylindrical phantoms were 3D printed from water equivalent PLA of varying diameters. The custom phantom was placed on solid water and measurements were carried out using NACP chamber at dmax depth. The CCF was defined as the ratio of the chamber reading with the custom phantom to that with solid water at the same depth. CCFs were measured for three electron energies (6MeV, 9MeV and 12MeV) and compared to calculations made in the Eclipse TPS. CCFs are then applied to ClearCalc calculations for patient cases to compare against the Eclipse MU calculation. Varian’s Eclipse API scripting is used to assist in calculating the patient curvature for the IMU calculations. The mean difference between the measured and TPS calculated CCFs was -0.6% with a maximum difference of 7.8% for the smallest diameter phantom (2.8cm) with 6MeV energy. The measured CCF’s can be used for correcting surface curvature to improve independent MU calculations for electron plans.
Matching root water uptake patterns to fine root and soil water distributions
Authors: Zhu Wei , Zhao Dehai , Di Nan , Li Doudou , Zhou Ou , Sun Yiming , Jia Liming , Ding Changjun , Xi Benye 
Published: August 2023

Abstract
Aims
Exploring the relationships between water uptake, fine root, and soil water is essential for many fields, such as plant physiology, ecological hydrology, and water conservancy.
Methods
In four Populus tomentosa stands with different ages (young and mature) and stand structures (low and high stand density), we matched root water uptake patterns obtained from stable isotope techniques with fine root and soil water distributions, respectively. The effects of soil water content and meteorological factors on these matching degrees were further explored.
Results
It was found that the water uptake pattern was closer to soil water distribution than to fine root distribution in mature stands, while water uptake pattern matched both fine root and soil water distribution closely in young stands. Increased competition intensity within a stand would suppress the matching degree between water uptake patterns and fine root distribution, but would have little effect on the matching degree between water uptake patterns and soil water distribution. There is a relatively high positive or negative correlation between these two matching degrees in all four stands. Compared with the direct effect of soil water content, the influence of meteorological factors on matching degrees was relatively weak.
Conclusion
The results indicate that the expression of water uptake function of the young stand depends on a combination of root structure and available water resources, while the water uptake function of mature stands is driven more by available water resources rather than root structure.
Genome-wide association study for temperature-response and photo-thermal interaction of flowering time in soybean using a panel of cultivars with diverse maturity groups
Authors: Wu Tingting , Wen Huiwen , Zhang Xinyue , Jia Hongchang , Xu Cailong , Song Wenwen , Jiang Bingjun , Yuan Shan , Sun Shi , Wu Cunxiang , Han Tianfu 
Published: August 2023

Abstract
The flowering transition is a crucial component of soybean (Glycine max L. Merr.) development. The transition process is regulated by photoperiod, temperature, and their interaction. To examine the genetic architecture associated with temperature- and photo-thermal-mediated regulation of soybean flowering, we here performed a genome-wide association study (GWAS) using a panel of 201 soybean cultivars with maturity groups ranging from MG 000 to VIII. Each cultivar was grown in artificially-controlled photoperiod and different seasons in 2017 and 2018 to assess the thermal response (TR) and the interactive photo-thermal response (IPT) of soybean flowering time. The panel contained 96,299 SNPs with minor allele frequencies > 5%; 33, 19, and 49 of these SNPs were significantly associated with only TR, only IPT, and both TR and IPT, respectively. 21 SNPs were located in or near previously reported quantitative trait loci (QTL) for first-flowering; 16 SNPs were located within 200 kb of the main-effect flowering genes GmFT2a, GmFT2b, GmFT3a, GmFT3b, GmFT5a, GmFT5b, GmCOL2b, GmPIF4b, and GmPIF4c, or near homologs of the known Arabidopsis thaliana flowering genes BBX19, VRN1, TFL1, FUL,AGL19, SPA1, HY5, PFT1, and EDF1. Natural non-synonymous allelic variations were identified in the candidate genes Glyma.08G302500 (GmHY5), Glyma.08G303900 (GmPIF4c), and Glyma.16G046700 (GmVRN1). Cultivars with different haplotypes showed significant variations in TR, IPT, and flowering time in multiple environments. The favorable alleles, candidate genes, and diagnostic SNP markers identified here provide valuable information for future improvement of soybean photo-thermal adaptability, enabling expansion of soybean production regions and improving plant resilience to global climate change.
Impact of climatic factors on incidence of rice leaf folder (Cnaphalocrosis medinalis, Lepidoptera: Crambidae) in rainfed rice crop of North-East India
Authors: Das Purnima , Borah Binita , Gharphalia Bikash Jyoti , Saikia Hemanta , Saikia Priyanka , Mudoi Abhilisa 
Published: August 2023

Abstract
To investigate and quantify the impact of abiotic factors viz., temperature, relative humidity, rainy days, rainfall and bright sunshine hours on the seasonal incidence of rice leaf folder (Cnaphalocrosis medinalis Guenee, Lepidoptera: Crambidae) in rice ecosystem of North-East India.The infestation of rice leaf folder was monitored in the rice field by visual counting of folded leaves as well as the incidence of the pest with larvae and were correlated with the weather parameters during the study periods. Results revealed that leaf folder damage percentage culminated at 34th to 36th Standard Meteorological Week (SMW) during the sali seasons (kharif crop) of the stipulated five-year period i.e 2016–2020. Maximum temperature, minimum temperature, morning relative humidity and rainy days significantly effect on leaf folder infestation. Maximum temperature, minimum temperature, morning relative humidity, rainy days and rainfall together contributed about 58–87 per cent of total variation in leaf folder infestation. The principal component analysis was conducted and Minimum temperature exhibit prominence significant effect on the incidence of rice leaf folder among the climatic factors. The climatic factors and its changes during the crop cycle plays crucial on survival and infestation of rice leaf folder.
Predictive maintenance using deep learning and frequency harmonics analysis
Authors: Gomez Herman Maicol , Perafan-Villota Juan Carlos 
Published: August 2023

Abstract
Reciprocating machines are critical assets in factories. Thus, predictive maintenance (PdM) plays a crucial role in avoiding unbudgeted costs. Firstly, a preliminary diagnostic is carried out by qualified personnel who interpret data from sensors based on the knowledge learned from maintenance manuals and their expertise, which can turn into a spend and monotonous task. Currently, machine learning and signals analysis hold great potential in predictive maintenance, allowing assist experts to make appropriate decisions in advance. However, there are many reasons why so far it is not something easy to do or that is scalable. In this paper, we propose a low-cost architecture based on a convolutional neural network feed by vibration data, which achieves high performance in identifying the current asset health, getting robust features regardless of little information available.
Isolation, anticancer potency and camptothecin – producing ability of endophytic fungi isolated from Ixora chinensis
Authors: Doan Nhung Thi , Le Dung Thi , Ho Anh Ngoc , Ho Thuong Thi , Do Thao Thi , Hoang Ha , Nguyen Mau Hung , Bui Thanh Mai , Chu Hoang Ha 
Published: August 2023

Abstract
Purpose
Camptothecin (CPT) is an important alkaloid used for anticancer treatment. It is mainly produced by two endangered and overharvested Camptotheca acuminata and Nothapodytes nimmoniana plants. Endophytic fungi are promising alternative sources for CPT production. This study aimed to evaluate the distribution of fungal endophytes in Ixora chinensis, their cytotoxic properties and camptothecin – producing ability.
Methods
In the present study, fungi residing within explants of Ixora chinensis were isolated and their CPT-producing capability of their endophytes was verified via TLC, HPLC, LC-HRMS and NMR analyses and compared with standards. In addition, MTT and SRB assays were selected to test the anticancer effect.
Results
The endophytic fungi collection of 62 isolates were assigned to 11 genera, with four common genera (Diaporthe, Phyllosticta, Colletotrichum, and Phomopsis) and seven less common genera (Penicillium, Botryosphaeria, Fusarium, Pestalotiopsis, Aspergillus, and Didymella). Moreover, the anticancer activity of extracts was assessed against human lung carcinoma (A549). Among 8 potential extracts, only Penicillium sp. I3R2 was found to be a source of CPT, while the remaining seven extracts have not been detected to contain CPT. Thus, other prominent endophytic fungi might be potential candidates of phytochemicals with anticancer properties.
Conclusions
The present study identified novel endophytic fungi from I. chinensis as prospective sources for camptothecin and other potential bioactive compounds.
A Case Report of Warm Autoimmune Hemolytic Anemia
Authors: Raulji Jay , Thakore Dhruvin 
Published: August 2023

Abstract
Hemolytic anemia is a hematologic disorder characterized by the premature destruction of red blood cells (RBCs). This can lead to a shortage of RBCs, which can cause a variety of symptoms, including fatigue, shortness of breath, and jaundice.
The destruction of RBCs can occur in two main ways: intravascular hemolysis and extravascular hemolysis. In intravascular hemolysis, the RBCs are destroyed within the bloodstream. This can be caused by factors such as immune-mediated hemolysis, mechanical trauma, or infections. In extravascular hemolysis, the RBCs are destroyed by the spleen and other organs of the reticuloendothelial system. This can be caused by factors such as hereditary defects in RBCs, infections, and medications.
The diagnosis of hemolytic anemia is made based on a clinical evaluation and a variety of laboratory tests. The clinical evaluation should focus on identifying the symptoms and signs of hemolytic anemia, as well as any underlying conditions that may be causing the hemolysis. The laboratory tests that are typically performed include a complete blood count (CBC), a direct antiglobulin test (DAT), a haptoglobin test, and a bilirubin test.
The treatment of hemolytic anemia depends on the underlying cause. In some cases, no treatment is necessary. In other cases, treatment may include medications, blood transfusions, or surgery.
The prognosis for hemolytic anemia varies depending on the underlying cause. Some types of hemolytic anemia are chronic and lifelong, while others can be cured.
Donepezil and Embelin loaded Nanostructured lipid carriers for direct brain delivery as an intervention for Alzheimer’s disease: Formulation design, Optimization and Evaluation
Authors: Ali Mohd Humair , Alam Ozair , Ali Asad , Ali Mohd Uzair , Parvez Suhel , Aldosari Eman , Baboota Sanjula , Ali Javed 
Published: August 2023

Abstract
Donepezil hydrochloride (DPL) and Embelin (EMB) loaded Nanostructured Lipid Carriers (NLCs) have been developed and optimized to achieve optimal drug loading, safer nasal delivery, effective neuronal/cell uptake, enhanced brain accessibility, controlled release, and desired therapeutic effect. Molecular docking studies demonstrated that both drugs bind effectively to AchE with interaction energies of -48.5319 and − 65.7525, respectively, indicating a synergistic approach. The hydrophobic interactions with target proteins facilitate the transportation of drugs through brain hydrophobic channels to provide a desired pharmacological response. N2a cell line investigation advised a 1:1 ratio of DPL and EMB to have the greatest possible synergistic effect based on the MTT assay. NLCs were fabricated by hot emulsification probe sonication method and optimized using QbD-based Central Composite Rotatable Design (CCRD). Optimized NLCs with a diameter of 180.2 nm were suitable for axonal uptake. A low PDI score of 0.37 and ZP of -12 mV indicated a uniform monodisperse system with persistent and stable dispersion properties. The NLCs demonstrated sustained drug release, DPL released at 90.72 ± 1.00 percent and EMB at 81.30 ± 0.52 percent in 24 hours. The Korsemeyer-Peppas model proved to be the most accurate fit due to its strong correlation. Ex vivo permeation and CLSM studies revealed superior goat nasal mucosa penetration of NLCs over suspension with a higher fluorescence level, up to 35 µm. NLCs treated nasal mucosa exhibited no erosion or interstitial gaps in the histopathological study. Moreover, NLCs were nontoxic and non-irritating, with a HET CAM score of 0.68 ± 0.05, indicating safe nasal delivery. The cellular uptake study showed a preponderance of the NLCs in the Cell's cytoplasm, indicating ready uptake by N2a cells. Hence, intranasal therapy with the DPL and EMB-loaded NLCs could be a practical and promising implementation. Further in vivo, and clinical studies will be required to establish the formulation's efficacy in treating Alzheimer’s disease (AD).