bluuebunny/crossref_metadata_2025_split huggingface dataset:
New and Old Criteria for diagnosing Celiac Disease: an observational study.
Authors: Accomando Salvatore , Piazza Ilenia Rita , Cacciatore Francesca , Corsello Giovanni , Giuffrè Mario 
Published: January 2023

Abstract
Background
The aim of our study is to compare two groups of celiac patients: the first one in which diagnosis was based on the New 2012 ESPGHAN criteria, and the second one based on the 1991 Revised Criteria in order to find relevant difference for sex, M/F ratio, age at diagnosis, clinical features at the onset, presence and prevalence of concomitant autoimmune disorders.
Methods
Our study involves 61 patients having the CD onset from February 2013 to February 2020. The patients who received diagnosis according to the New 2012 ESPGHAN Criteria were enrolled in group 1. The patients who received diagnosis according to the Revised 1991 Espghan Criteria were enrolled in group 2. Prevalence of comorbidities was analysed through chi-square test.
Results
In group 1 the prevalence of comorbidities such as IDDM and thyroiditis was of 53%, while in group 2 was only of 24%. Analysing the difference of the IDDM prevalence between the two groups we found a relevant difference. In the same way also the prevalence of thyroiditis was significantly different. In group 1, male patients, in particular, seem to have a higher incidence of CD related autoimmune disorders.
Conclusions
An increased prevalence of IDDM, thyroiditis and JIA in the first group shows that the new diagnostic criteria could expose patients to a greater lengthy of disease activity responsible for the onset of such comorbidities. Further studies should be carried out on more numerous samples of patient in order to confirm this data.
(Almost) all the humpback whales of the North Pacific: A collaborative and comprehensive photo-ID dataset
Authors: Cheeseman Ted , Southerland Ken , Acebes Jo Marie , Audley Katherina , Barlow Jay , Bejder Lars , Birdsall Caitlin , Bradford Amanda , Byington Josie , Calambokidis John , Cartwright Rachel , Cedarleaf Jen , Chavez Andrea Jacqueline García , Currie Jens , De Weerdt Joëlle , Doe Nicole , Doniol-Valcroze Thomas , Dracott Karina , Filatova Olga , Finn Rachel , Flynn Kiirsten , Ford John , Frisch-Jordán Astrid , Gabriele Christine , Goodwin Beth , Hayslip Craig , Hildering Jackie , Hill Marie , Jacobsen Jeff , Jones Meagan , Kobayashi Nozomi , Lyman Edward , Malleson Mark , Mamaev Evgeny , Loustalot Pamela Martínez , Masterman Annie , Matkin Craig , McMillan Christie , Moore Jeff , Moran John , Neilson Janet , Newell Hayley , Okabe Haruna , Olio Marilia , Pack Adam , Palacios Daniel , Pearson Heidi , Quintana-Rizzo Ester , Barragán Raul Fernando Ramírez , Ransome Nicola , Sharpe Fred , Shaw Tasli , Stack Stephanie , Staniland Iain , Straley Jan , Szabo Andrew , Teerlink Suzie , Titova Olga , Urban Jorge , Aswegen Martin van , Vinicius Marcel , von Ziegesar Olga , Witteveen Briana , Wray Janie , Yano Kymberly , Zwiefelhofer Denny , Rosales-Nanduca Hiram , Jiménez-López M. Esther , Clapham Phil 
Published: January 2023

Abstract
We present an ocean-basin-scale dataset that includes tail fluke photographic identification (photo-ID) and encounter data for the majority of living individual humpback whales (Megaptera novaeangliae) in the North Pacific Ocean. The dataset was built through a broad collaboration combining 39 separate curated photo-ID catalogs supplemented with community science data. All available images were compared using a recently developed machine learning artificial intelligence image recognition algorithm able to rapidly and accurately detect matches between individuals. For the study period of 2001 to 2021, a total of 27,956 unique individuals were documented in 157,379 encounters, with each individual encountered, on average, in 5.6 sampling periods (i.e., breeding and feeding seasons), and with an annual average of 87.1% of whales encountered in more than one season. The combined dataset and image recognition tool represents a living and accessible resource for collaborative, basin-wide studies of a keystone marine mammal in a time of rapid ecological change.
Novel antibacterial and apatite forming restorative composite resin incorporated with hydrated calcium silicate
Authors: Yang Song-Yi , Han A Ruem , Choi Ji-Won , Kim Kwang-Mahn , Kwon Jae-Sung 
Published: January 2023

Abstract
Background. White Portland cement is calcium silicate material. It exhibits antibacterial properties and is biocompatible. In addition, calcium silicate-based materials are known to release calcium ions and form apatite. The purpose of this study was to develop a novel bioactive restorative resin composite with antibacterial and apatite forming properties to prevent tooth caries at the interface of teeth and restorative materials, by incorporation of hydrated calcium silicate (hCS) derived from white Portland cement.
Methods. To prepare the experimental composite resins, a 30 wt.% light-curable resin matrix and 70 wt.% filler, which was mixed with hCS and silanized glass powder were prepared in following concentrations: 0, 17.5, 35.0 and 52.5 wt.% hCS filler. The depth of cure, flexural strength, water sorption, solubility, and antibacterial effect were tested. After immersion in artificial saliva solution for 15, 30, 60, and 90 days, ion concentration by ICP-MS and apatite formation using SEM-EDS, Raman spectroscopy and XRD from experimental specimens were analyzed.
Results. All experimental groups showed clinically acceptable depths of cure and flexural strength for the use as the restorative composite resin. Water sorption, solubility, released Ca and Si ions increased with the addition of hCS to the experimental composite resin. Experimental groups containing hCS showed greater antibacterial effects compared with the 0 wt.% hCS filler group (p<0.05). The 52.5 wt.% hCS filler group produced precipitates mainly composed of Ca and P detected as hydroxyapatite after immersion in artificial saliva solution for 30, 60, and 90 days.
Conclusions. This results show that composite resins containing hCS filler is effective in antibacterial effects. hCS has also apatite formation ability for reducing gap size of microleakage by accumulating hydroxyapatite precipitates at the restoration-tooth interface. Therefore, novel composite resin containing hCS is promising bioactive resin because of its clinically acceptable physiochemical properties, antibacterial properties, and self-sealing potential for prevention of microleakage for longer usage of restorations.
Genome-wide association study (GWAS) of host germline variation and the gut microbiome: The Multiethnic Cohort Study
Authors: Hullar Meredith , Curtis Keith , Li Yuqing , Lampe Johanna , Jenkins Isaac , Randolph Timothy , Lim Unhee , Wilkens Lynn , Marchand Loic Le , Kristal Bruce , Monroe Kristine , Zhao Kechen , Stram Daniel , Cheng Iona 
Published: January 2023

Abstract
Patterns of microbiome diversity vary across human populations largely driven by lifestyle and environmental factors. However, differences in genetically-encoded traits in the host may also be important in shaping the microbiome and related health outcomes. We report results from a GWAS of the gut microbiome in 5,202 individuals from the Multiethnic Cohort Study, including African American, Japanese American, Native Hawaiian, Latino, and White individuals. Genotyping was derived from previous studies (n = 3,337) using various Illumina Infinium arrays (660,000 to 2.5 million SNPs) and the MEGA EX array (n = 1,865). Single nucleotide polymorphism (SNP) imputation was conducted using a cosmopolitan reference panel from the 1000 Genomes Project. The stool microbiome was assessed by paired-end sequencing (Illumina MiSeq) of the16S rRNA gene (V1 − 3). SNP-genera association tests were conducted using ordinal logistic regression with quintiles of bacterial abundance regressed on SNPs, adjusted for age, ancestry estimates, season of sample collection, batch, and genotyping study, using a genome-wide statistical significance threshold of p < 5*10− 8. We identified associations between 53 SNPs in 11 human chromosomes and 16 bacterial/archaeal genera at p < 5*10− 8.The SNPs in coding regions were categorized into broad categories: human genes known to be exploited by bacterial pathogens; genes involved in nutrition, obesity, diabetes, and cancer; and immune function. Most significantly, Bifidobacterium abundance was associated with 2 known SNPs on chromosome 2 (rs182549 p = 3.8*10− 11; rs4988235 4.8*10− 11) in the MCM6 gene that were involved in lactose intolerance overall and in Latinos (rs182549 p = 4.12*10− 09 and rs4988235 p = 6.90*10− 09) and replicated in other studies. A significant association between Coriobacteriales and CDH18 (rs7701767,p = 1.5*10− 08) was also replicated in East Asian cohorts. Genetic variants in non-coding regions were primarily associated with host defenses against infection via solubilizing pathogen cell membranes, restricting growth of intracellular pathogens, and triggering inflammation though innate immune response. Fusicatenibacter was associated with a SNP (rs8067381,p = 1.63*10− 6) found in non-coding regions between SOCS7 and ARHGAP23 and replicated in several East Asian cohort. Expansion into human cohorts to include racial and ethnic diversity in host genetics and microbiome interactions to support an understanding of health outcomes across the human population.
Phospholipid Bilayer Inspired Sandwich Structural Nanofibrous Membrane for Selective Water Harvesting and Release
Authors: Lai Yuekun , Yu Zhihua , Li Shuhui , Zhang Jichao , Zhou Zhijuan , Qin Ziqi , Liu Xiaojie , Fu Shaohai 
Published: January 2023

AbstractAtmospheric water harvesting (AWH) has been broadly exploited to meet the challenge of water shortage. Despite the significant achievements of AWH, the unendurable water harvesting, inferior water release performance, and short service life hinder practical applications. Herein, inspired by the unique selective permeability of the phospholipid bilayer, a sandwich structural polyacrylonitrile nanofibrous membrane (San-PAN) was fabricated to improve the water sorption/desorption ability. The special wettability of the sandwich structure (hydrophobic-hydrophilic-hydrophobic) could confine the captured water in the hydrophilic layer and prevent the leakage of the hygroscopic salt, achieving continuable and recyclable water sorption/desorption. Notably, attributed to the high thermal conductivity of Ag particles in the inner layer, the water condensation, and heat transfer ability of San-PAN were remarkably enhanced. As demonstrated, the as-prepared AWH devices presented a high-efficient adsorption kinetics (4.08 g g-1at 25°C and 90% relative humidity). Thus, this work strengthens the understanding of the water sorption/desorption process and opens an avenue to the practical applications of wearable outdoor water supply equipment.
A Multicentre, Patient- and Assessor-blinded, Non-inferiority, Randomised and Controlled Phase Ii Trial to Compare Standard and Torque Teno Virus-guided Immunosuppression in Kidney Transplant Recipients in the First Year After Transplantation: Ttv Guide It
Authors: Haupenthal Frederik , Rahn Jette , Maggi Fabrizio , Gelas Fanny , Bourgeois Philippe , Hugo Christian , Jilma Bernd , Böhmig Georg A , Herkner Harald , Wolzt Michael , Doberer Konstantin , Vossen Matthias , Focosi Daniele , Neuwirt Hannes , Banas Bernhard , Banas Miriam , Budde Klemens , Viklicky Ondrej , Malvezzi Paolo , Rostaing Lionel , Rotmans Joris I , Bakker Stephan J.L , Eller Kathrin , Cejka Daniel , Perez Alberto Molina , Rodriguez-Arias David , König Franz , Bond Gregor 
Published: January 2023

Abstract
Background

Immunosuppression after kidney transplantation is mainly guided via plasma tacrolimus trough level, which cannot sufficiently predict allograft rejection and infection. The plasma load of the non-pathogenic and highly prevalent Torque Teno Virus (TTV) is associated with the immunosuppression of its host. Non-interventional studies suggest the use of TTV load to predict allograft rejection and infection. The primary objective of the current trial is to demonstrate the safety, tolerability and preliminary efficacy of TTV-guided immunosuppression.

Methods

For this purpose, a randomised, controlled, interventional, two-arm, non-inferiority, patient- and assessor-blinded, investigator-driven phase II trial was designed. A total of 260 stable, low-immunological-risk adult recipients of a kidney graft with tacrolimus-based immunosuppression and TTV infection after month 3 post-transplantation will be recruited in 13 academic centres in six European countries. Subjects will be randomised in a 1:1 ratio (allocation concealment) to receive tacrolimus either guided by TTV load or according to the local centre standard for 9 months. The primary composite endpoint includes the occurrence of infections, biopsy-proven allograft rejection, graft loss or death. The main secondary endpoints include estimated glomerular filtration rate, graft rejection detected by protocol biopsy at month 12 post-transplantation (including molecular microscopy), development of de novo donor-specific antibodies, health-related quality of life and drug adherence. In parallel, a comprehensive biobank will be established including plasma, serum, urine and whole blood. The date of the first enrolment was August 2022 and the planned end is April 2025.

Discussion

The assessment of individual kidney transplant recipient immune function might enable clinicians to personalise immunosuppression, thereby reducing infection and rejection. Moreover, the trial might act as a proof of principle for TTV-guided immunosuppression and thus pave the way for broader clinical applications, including as guidance for immune modulators or disease-modifying agents.
Trial registration: EU CT-Number: 2022-500024-30-00
Magnetoelectric Coupling in multiferroics Probed by Optical Second Harmonic Generation
Authors: Xu Shuai , Wang Jiesu , Chen Pan , Jin Kuijuan , Ma Cheng , Wu Shiyao , Guo Er-Jia , Ge Chen , Wang Can , Xu Xiulai , Yao Hongbao , Wang Jinyi , Xie Donggang , Wang Xinyan , Chang Kai , Bai Xuedong , Yang Guozhen 
Published: January 2023

Abstract
Magnetoelectric coupling, as a fundamental physical nature and with the potential to add functionality to devices while also reducing energy consumption, has been challenging to be probed in freestanding membranes or two-dimensional materials due to their instability and fragility. In this paper, we report a magnetoelectric coupling probed by optical second harmonic generation with external magnetic field, and show the manipulation of the ferroelectric and antiferromagnetic orders by the magnetic and thermal fields in BiFeO3 films epitaxially grown on the substrates and in the freestanding ones. Here we define an optical magnetoelectric-coupling constant, denoting the ability of controlling light-induced nonlinear polarization by the magnetic field, and found the magnetoelectric-coupling was suppressed by strain releasing but remain robust against thermal fluctuation for freestanding BiFeO3.
Pursuing carbon neutrality in China should enhance the resilience of its nitrogen-phosphorus coupled cycling network and also benefit food security
Authors: Luo Zimeng , Yu Yadong , Kharrazi Ali , Fath Brian , Matsubae Kazuyo , Liang Sai , Chen Dingjiang , Zhu Bing , Ma Tieju , Hu Shanying 
Published: January 2023

Abstract
Nitrogen (N) and phosphorus (P) are essential nutrients for living systems and play a central role in human food systems. While N and P pathways have been investigated individually in the literature, the N-P coupled cycling network has not received sufficient attention. The coupled nature of N and P cycling networks and the importance of their resilience for the future of food and energy systems is of critical importance for sustainable development. In this avenue, we use the material flow and ecological network analysis methods to construct the N-P coupled cycling network in China and evaluate its resilience. The results show that the resilience of China’s N-P coupled cycling network decreased during the 1980–2017 period and, given China’s goal of further carbon neutrality, resilience is expected to continue decreasing throughout the study period (until 2060). Furthermore, under our clean energy scenario, the N-P coupled cycling network will have a very substantial decrease in its resilience, especially in the N layer (by 20%). China’s socio-economic system also suffers the risk of great N emissions to the environment, thus disturbing the N cycle, and amplifying the conﬂict between energy and food systems given the scarcity of P. Our findings reveal trade-offs and synergies under future anthropogenic nexus scenarios and can equip policymakers to make more informed deliberations on the management of N and P cycling networks in China, e.g., reducing fertilizer use and food waste.
Expression and Purification of Functional SARS-CoV-2 RBD in E. coli for Therapeutic and Diagnostic Purposes
Authors: Ghaderi Hajarossadat , Shoari Alireza , Salehi Shima , Eskafi Ayda Hassanzadeh , Habibi-Anbouhi Mahdi , Cohan Reza Ahangari , Moazzami Reza , Behdani Mahdi 
Published: January 2023

Abstract
SARS-CoV-2 causes a severe respiratory disease known as COVID-19 and is responsible for a global viral pandemic. The SARS-CoV-2 receptor binding domain (RBD) is located on the spike protein (S), which is dedicated for identifying and binding to the angiotensin converting enzyme 2 (ACE2) receptor. The RBD is an important target for development of virus neutralizing antibodies, vaccines, and inhibitors. In this study, recombinant SARS-CoV-2 RBD was expressed in E. coli BL21 (DE3) and purified as well as its binding activity was determined. Purification was conducted by Ni-NTA column. ELISA and flow cytometry assays were conducted to evaluate the binding ability of recombinant RBD to different anti-RBD antibodies and native ACE2 receptor on HEK293A cells, respectively. ELISA results showed that antibodies produced in the human sera could bind to the recombinant RBD protein as well as the commercial anti-RBD antibody. Also, flow cytometry analysis showed that the recombinant RBD was able to bind to human ACE2 on the surface of HEK293A cells. Our outcomes displayed that the recombinant RBD expressed in E. coli strain has biological activity and can be used as an antigen for development of diagnosis kits and vaccines as well as a tool for screening drugs against SASR-CoV-2.
Perimenopausal Effects of Estradiol on Anhedonia and Psychosis Study (PEEPs): Study Protocol for a Neural and Molecular Mechanistic Clinical Trial {1}
Authors: Walsh Melissa J.M. , Gibson Kathryn , Hynd Megan , Eisenlohr-Moul Tory A. , Walsh Erin C. , Schiff Lauren , Jarskog Fred , Lalush David , Dichter Gabriel S. , Schiller Crystal E. 
Published: January 2023

Abstract
Background: The perimenopausal transition is accompanied by psychiatric symptoms in over 10% of women. Symptoms commonly include depressed mood and anhedonia and less commonly include psychosis. Psychiatric symptoms have been linked to the depletion and/or variability of circulating estradiol, and estradiol treatment reduces perimenopausal anhedonia and psychosis in some women. Estrogen fluctuations may disrupt function in the mesolimbic reward system in some women, leading to psychiatric symptoms like anhedonia or psychosis. The Perimenopausal Effects of Estradiol on Anhedonia and Psychosis Study (PEEPs) is a mechanistic clinical trial that aims to 1) identify relationships between perimenopausal-onset anhedonia and psychosis and neuromolecular markers of mesolimbic reward responses and 2) determine the extent to which estradiol treatment-induced changes in mesolimbic reward responses are associated with alleviation of perimenopausal onset anhedonia or psychosis.
Methods: This study will recruit 100 unmedicated women ages 44-55 in the late-stage perimenopausal transition, sampling across the range of mild-to-high anhedonia and absent-to-moderate psychosis symptoms. Patients will be randomized to receive either estradiol or placebo treatment for three weeks. Clinical outcome measures will include symptoms of anhedonia (measured with Snaith-Hamilton Pleasure Scale; SHAPS) and psychosis (measured with Brief Psychiatric Rating Scale; BPRS psychosis subscale) as well as neural markers of mesolimbic reward system functioning, including reward-related fMRI activation and PET-derived measure of striatal dopamine binding. Pre-treatment associations between 1) SHAPS/BPRS scores and 2) reward-related striatal dopamine binding/BOLD activation will be examined. Furthermore, longitudinal mixed models will be used to estimate 1) symptom and neuromolecular trajectories as a function of estradiol vs. placebo treatment and 2) how changes in reward-related striatal dopamine binding and BOLD activation predict variability in symptom trajectories in response to estradiol treatment.
Discussion: This clinical trial will be the first to characterize neural and molecular mechanisms by which estradiol treatment ameliorates anhedonia and psychosis symptoms during the perimenopausal transition, thus laying the groundwork for future biomarker research to predict susceptibility and prognosis and develop targeted treatments for perimenopausal psychiatric symptoms. Furthermore, in alignment with the National Institute for Mental Health Research Domain Criteria initiative, this trial will improve our understanding of a range of disorders characterized by anhedonia,psychosis, andreward system dysfunction.
Trial Registration: ClinicalTrials.gov Identifier: NCT05282277 {2a}
Antimicrobial peptide Mastoparan X has good activity against Escherichia coli in vitro and alleviates its pathogenicity in mice
Authors: Zhao Xueqin , Wang Lei , Zhu Chunling , Xue Xianghong , Xia Xiaojing , Wu Xilong , Bai Yueyu , Hu Jianhe 
Published: January 2023

Abstract
Background: Escherichia coli is a facultative anaerobic bacterium that exists in the gastrointestinal tract of humans and animals and can cause diarrhoea and enteritis. The antimicrobial peptide MPX is extracted from wasp venom and has a strong bactericidal effect on many bacteria. The aim of this study was to explore the mechanism by whichMPX kills E. coli in vitro, its effect on IPEC-J2 cells apoptosis and barrier function, and itstherapeutic effect on E. coli infection in mice.
Methods: In this study, the effects of MPX on E. coli were investigated in vitro, at cellular level and in vivo.
Results: The results showedthat the minimum inhibitory concentration (MIC) of MPX against E. coli was 31.25 µg/mL. Scanning electron microscopy results showed that the bacteria became smaller in size and the contents leaked after treatment with MPX. In addition, theNPN, PI and DiSC3(5) results showed that the fluorescence intensity was positively correlated with MPX. MPX significantly inhibited E. coli biofilm formation. Moreover, MPX effectively alleviated IPEC-J2 cell apoptosis, regulated ZO-1, Occludin, and Claudin-1 through theRac1 pathway. H&E staining results further found that MPX could alleviate the pathological damage in intestine. qRT–PCR results showed that MPX could increase the mRNA expression of TFF3 in the jejunum and colon.
Conclusions: This study is the first time to explore the mechanism by which MPX kills E. coli in vitro, laying the foundation for the development of new drugs for the treatment of bacterial infections.