bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Cardiac Index on Mortality in Patients with Septic Shock: A retrospective study from the MIMIC-IV Database
Authors: Ngan Chansokhon , Li Li , Wang Mingchun , Zeng Xueying , Yin Wanhong , Kang Yan 
Published: May 2023

Abstract
Background
Septic shock is a life-threatening condition that can lead to organ dysfunction and death. In the ICU, monitoring of cardiac index (CI) is commonly used to guide management and predict outcomes in septic shock patients. However, there is a lack of research on the association between CI and the risk of mortality in this patient population. Therefore, the aim of this study was to investigate the relationship between different levels of CI and mortality in septic shock patients.
Methods
Data analysis was obtained from the MIMIC-IV version 2.0 database. Sepsis and septic shock were primarily defined by sepsis-3, the third international consensus on sepsis and septic shock. CI was computed using cardiac output (CO) and body surface area (BSA). To evaluate the incidence of CI with respect to each endpoint (7-, 14-, 21-, and 28-day mortality), a restricted cubic spline curve function (RCS) was used. Analyses of KM curves, cox regression, and logistic regression were conducted separately to determine the relationship between various CI and 28-day mortality.
Results
This study included 1498 patients with septic shock. A U-shaped relationship between CI levels and risk of mortality in septic shock was found by RCS analysis (p < 0.001). CI levels in the range of 1.85–2.8 L/min/m2 were associated with a mortality hazard ratio (HR) < 1. In contrast, low CI and high CI levels had a significantly increased risk of mortality. According to the characteristics of CI, patients were divided into three groups low CI group (CI < 1.85 L/min/m2,n = 150), CI normal group (CI:1.85–2.80 L/min/m2, n = 904), and the high group(CI > 2.80 L/min/m2,n = 444). The KM curves, COX regression, and logistic regression analysis showed that the survival rate the of CI normal group was higher than the other groups. The risk factors of the low CI group, and high CI with ICU 28-day mortality were HR = 1.87 (95% CI: 1.01–3.49), and HR = 1.93 (95% CI: 1.26–2.97), respectively.
Conclusion
This study found that a cardiac index (CI) of 1.85–2.80 L/min/m2 was associated with the lowest risk of mortality in patients with septic shock. CIs that were lower or higher than this range were associated with an increased risk of mortality.
Development and validation of LncRNA prognostic index associated with immunogenic cell death in hepatocellular carcinoma
Authors: Shi Wen , Jia Weili , Nie Ye , Wang Yanfang , Mao Zhenzhen , Chen Chao , Zhao Zihao , Song Wenjie 
Published: May 2023

Abstract
This study aims to construct immunogenic cell death(ICD) related LncRNA signature to explore the prognosis and immune infiltration of hepatocellular carcinoma(HCC) patients. The Cancer Genome Atlas (TCGA) database was randomly divided into training and test sets, while co-expression analysis was used to find relevant LncRNA. Next, the least absolute choice operator (LASSO) and Cox regression analysis were performed, and a total of 6 relevant LncRNAs (GAL, HOXD13, CTSV, SLC6A3, NR0B1, DCAF8L1) were found to establish the signature. Kaplan-Meier analysis showed that expression of high-risk LncRNAs scores correlated with poor overall survival of HCCs. Then, a prognostic nomogram containing LncRNA features and clinicopathological features was constructed, which verified the good predictive performance of the model on the prognosis of HCC patients. Immune-related functions differed significantly between high-risk and low-risk groups. Tumor mutation burden (TMB) and immune checkpoint expression also differed significantly between the two groups. Finally, HCC patients with high-risk scores were more sensitive to several chemotherapy drugs. In conclusion, we have developed a new HCC classification system based on the characteristics of ICD, which has important clinical implications for assessing the prognosis and treatment of HCC patients.
A novel cancer-germline gene DAZL promotes progression and cisplatin resistance of non- small cell lung cancer by upregulating JAK2 and MCM8
Authors: Zhou Lin , Uyen Le Mai Nhu , Du Yan , Shen Xiandie , Li Jing , Xia Liang′e , Ning Yichong , Zhou Jianlin 
Published: May 2023

Abstract

BackgroundGermline-specific genes are usually activated in cancer cells and drive cancer progression; such genes are called cancer-germline or cancer-testis genes. Deleted in azoospermia-like (DAZL) is a germline-specific gene whose role in gametogenesis is well characterized, but whose expression and function in non-small cell lung cancer (NSCLC) is unknown.MethodsRNA-seq data from Genotype Tissue Expression and The Cancer Genome Atlas were used to analyze DAZL mRNA levels. An immunochemical assay was performed to detect DAZL protein levels in a lung tissue microarray. Cell viability, EdU staining, colony formation, wound healing, and invasion assays were performed for in vitro functional analysis. The in vivo effect of DAZL on tumor growth was investigated using a subcutaneous murine xenograft model. Quantitative proteomics was performed to explore the potential targets of DAZL.ResultsDAZL was exclusively expressed in testis among normal human tissues but ectopically expressed in NSCLC tissues. Testis and NSCLC cells expressed the shorter and longer transcript variants of the DAZL gene, respectively. Overexpression of the longer DAZL transcript promoted tumor growth in a murine xenograft model.Silencing DAZL suppressed viability, colony formation, migration, and invasion of NSCLC cells. Mechanistically, DAZL binds to the mRNAs of Janus kinase 2 (JAK2) and minichromosome maintenance 8 (MCM8) to upregulate their translation. The JAK2 inhibitor fedratinib attenuated the effects of DAZL overexpression on cell viability, colony formation, migration, and invasion, while silencing MCM8 counteracted the effects of DAZL overexpression on cisplatin-damaged DNA synthesis and the half-maximal inhibitory concentration of cisplatin in NSCLC cells.ConclusionDAZL was identified as a novel cancer-germline gene that enhances translation of JAK2 and MCM8 to promote NSCLC progression and cisplatin resistance, respectively. These results suggest that DAZL is a potential therapeutic target in NSCLC.
Ribosomal S6 kinase 4 (RSK4) tumor suppressor gene promoter methylation status in ovarian cancer
Authors: Arechavaleta-Velasco Fabian , Dominguez-Lopez Pablo , Acosta-Jimenez Ernesto , Diaz-Cueto Laura 
Published: May 2023

Abstract
Background
Previously, we reported lower RSK4 mRNA and protein levels in malignant ovarian tumors compared to normal and benign ovarian tissues. Also, we observed a significant inverse correlation between the advanced ovarian cancer stages and RSK4 mRNA levels. We did not investigate the mechanisms involved in RSK4-reduced expression in ovarian cancer. Thus, this study investigates whether RSK4 promoter methylation in ovarian cancer tissues is responsible for its low expression. Additionally, the reactivation of RSK4 expression and its effect was studied in ovarian cancer cell lines.
Methods and results
RSK4 promoter methylation percentage in malignant and benign ovarian tumors and normal ovary tissues was determined by combined bisulfite restriction analysis. The reactivation of RSK4 expression by decitabine treatment was studied in OVCAR3, SKOV3, TOV-112D, and TOV-21G cells by Western blotting. Cell proliferation was determined by XTT. A significantly high methylation percentage of the RSK4 promoter was observed among malignant and benign ovarian tumors but not in normal ovarian tissue. RSK4 promoter methylation was not associated with age, histological subtype, or stages of ovarian cancer. RSK4 promoter methylation correlates weakly but not significantly with RSK4 protein expression. No correlation was shown between RSK4 methylation and RSK4 mRNA expression. Decitabine induces RSK4 reactivation in all cell lines. However, cell proliferation was reduced only in TOV-112D cells.
Conclusion
These data indicate that although RSK4 promoter methylation is increased in malignant ovarian tumors, this mechanism is unlikely to regulate its expression in ovarian cancer. RSK4 reactivation reduced cell proliferation only in the endometroid histological subtype.
Clinical Application of Chromosomal Microarray Analysis in High-Risk Pregnancies
Authors: Tao Hehua , Su Jingna , Zhao Li , Zhao Xin , Guo Caiqin , Xiao Jianpin , Liu Jun , Yang Lan 
Published: May 2023

Abstract
Objective To evaluate the value of chromosomal microarray analysis (CMA) in the prenatal diagnosis of high-risk pregnancies.Methods A total of 3459 pregnant women admitted to the Wuxi Maternal and Child Health Hospital between June 2020 and August 2022 were selected for this study. Both G-banding karyotype analysis and CMA were offered to all patients who underwent invasive prenatal diagnosis. Short tandem repeat (STR) analysis was performed to eliminate maternal contamination for interpretation.Results Chromosomal aberrations, including aneuploidies or triploid and mosaic aberrant karyotypes and copy number variants (CNVs) ≥ 5Mb, were detected by both G-banding karyotyping and CMA in 174 (5.0%) cases. A total of 326 (9.4%) cases with normal karyotypes were reported as CNVs in CMA, including 71 (2.1%) cases with pathogenic and likely pathogenic CNVs and 217(6.3%) cases with variants of uncertain significance (VOUS). On the other hand, 11 inversions, 23 balanced translocations, and 22 other chromosomal rearrangements were detected by karyotyping with normal CMA results.Conclusion The top 3 detection rates of different indications by CMA or karyotyping were 43.5% for NIPT high risk, 17.5% for abnormal ultrasound, and 17.7% for other indications. CMA is an effective diagnostic tool for high-risk pregnancies in perinatal medicine.
Convolutional neural network-based program to predict lymph node metastasis of non-small cell lung cancer using 18F-FDG PET
Authors: Kidera Eitaro , KOYASU SHO , Hirata Kenji , Hamaji Masatsugu , Nakamoto Ryusuke , Nakamoto Yuji 
Published: May 2023

Abstract
Purpose To develop a convolutional neural network (CNN)-based program to analyse maximum intensity projection (MIP) images of 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) positron emission tomography (PET) scans, aimed at predicting lymph node metastasis of non-small cell lung cancer (NSCLC), and to evaluate its effectiveness in providing diagnostic assistance to radiologists.Methods We obtained PET images of NSCLC from public datasets, including those of 435 patients with available N-stage information, which were divided into a training set (n = 304) and a test set (n = 131). We generated 36 maximum intensity projection (MIP) images for each patient. A residual network (ResNet-50)-based CNN was trained using the MIP images of the training set to predict lymph node metastasis. Lymph node metastasis in the test set was predicted by the trained CNN as well as by two radiologists (R1 and R2) twice: first without and second with CNN assistance. Diagnostic performance metrics, including accuracy, were calculated, and reading times were recorded.Results In the test set, 67 (51%) patients exhibited lymph node metastases and the CNN yielded 0.748 predictive accuracy. The accuracy of the radiologists increased from 0.718 to 0.740 for R1, and from 0.679 to 0.733 for R2 with CNN assistance. The mean prediction time was significantly reduced with CNN assistance, decreasing from 11.1 to 6.9 s (p < 0.001) for R1 and from 6.3 to 3.2 s (p < 0.001) for R2.Conclusion The CNN-based program could potentially assist radiologists in predicting lymph node metastasis, increase diagnostic performance, and reduce the time for diagnosis.
Analysis of the Effects of Temperature Variation on Fish Catch Along the Colombian Pacific Coast: A Stochastic Model
Authors: Martínez-Salinas Erika Johanna , Arunachalam Viswanathan , Ríos-Gutierrez Andrés , Selvaraj John Josephraj 
Published: May 2023

Abstract
Climatic factors affect biotic and abiotic elements that impact the abundance and distribution of fish species, particularly in the areas where they inhabit. In this article, we propose a stochastic model incorporating stochastic differential equations to analyze the behavior, over time, between the observed changes in sea surface temperature and its relationship with the abundance of one of the Mahi Mahi fish on a specific area of the Colombian Pacific coast. We estimate the proposed model parameters, sea surface temperature values, species stock, and species catch under study between the years 2000-2012 using the maximum likelihood and the Euler-Maruyama method. Using real data, we illustrate the proposed model’s numerical simulation and sensitivity analysis.
Tumor-Related Epilepsy and Post-Surgical Outcomes: Tertiary Hospital Experience in Vietnam
Authors: Le Viet-Thang , Nguyen Anh Minh , Pham Tuan Anh , Nguyen Phuc Long 
Published: May 2023

Abstract
Purpose: Seizures have a significant impact on the quality of life of those who suffer. This study aimed to evaluate the variables that influence the incidence of seizures during the perioperative period and effective measures to enhance epilepsy outcomes among individuals undergoing surgical resection of brain tumors.
Methods: The authors carried out a prospective observational analysis of all patients who experienced seizures before their brain tumor surgery at UMC, HCMC between 2020 and 2022.
Results: 54 cases presented with seizures were enrolled for the study, generalized seizure was the most prevalent seizure type (61.1%), followed by focal seizure (29.6%). Low-grade gliomas and frontotemporal lobe tumors increase the postoperative risk of seizure. Other predictive factors are a prolonged history of seizure, especially resistant epilepsy and major peritumoral edema. In contrast, gross total resection reduces postoperative seizure incidence. There was correlation between Ki67 proliferation index and seizure incidence in both low-grade and high-grade gliomas. ECoG made insubstantial difference in enhancing the epilepsy surgery outcome. Overall, 88.9% of patients were seizure-free at 6 months of follow-up (Engel Class I), 7.4% were almost seizure-free (Class II), and 3.7% had significant improvement (Class III), figures for 12-month follow-up were 87.0%, 9.3%, and 3.7% respectively.
Conclusion: The majority of patients presented with seizures are those who were diagnosed with glioma. Postoperatively, most of the patients were seizure-free or had meaningful improvement. A shorter history of seizure and gross-total resection appear to be associated with a favorable prognosis for seizure control.
An Energy Consumption Modeling Method for DED-based Hybrid Additive Manufacturing
Authors: Hasan Md Rabiul , Liu Zhichao , Rahman Asif 
Published: May 2023

Abstract
The awareness of energy consumption is gaining much more attention in manufacturing due to its economic and sustainability benefits. An energy consumption model is needed for quantifying the consumption and predicting the impact of various process parameters in manufacturing. This paper aims to develop an energy consumption model based on Specific Energy Consumption (SEC) for Direct Energy Deposition (DED) based Hybrid Additive Manufacturing (HAM). Three significant factors, including scanning speed, laser power, feed rate, and their interactions, are analyzed to investigate their effect on energy consumption. The results suggest that all the factors have a strong influence, but their interaction effects have a weak influence on the energy consumption for HAM. Among the three process parameters, laser power has the most significant effect on energy consumption. Based on the regression analysis result, this study recommends high scanning speed while the laser power and feed rate should be low to reduce energy consumption. Also, the idle time has significant energy consumption during the whole HAM process.
Preparation and Enantiomeric Separation of L-Pro-L-Phe-L-Val-L-Leu Peptide Stationary Phases
Authors: Guo Xinyuan , Shang Panpan , Wei Benben , Du Wenrong , Lan Yong , Bai Ligai , Qiao Xiaoqiang , Ma Zhengyue 
Published: May 2023

Abstract
This study presents the development of three new chiral stationary phases (CSP). Three new peptides CSPs with a main chain of L-proline (L-Pro), L-phenylalanine (L-Phe), L-valine (L-Val) and L-leucine (L-Leu) and different terminals of L-citrulline (L-Cit), L-lysine (L-Lys) and L-tryptophan (L-Typ) immobilized on 3-aminopropyltrimethoxysilane modified silica gel (APS) were prepared, furthermore, successful analyses and characterizations were conducted using Fourier transform infrared spectra, elemental analysis, and thermogravimetric analysis. After this, the enantioselective performance of the three L-Pro-L-Phe-L-Val-L-Leu peptide stationary phases columns were evaluated. The evaluation used 9 racemic compounds under normal-phase high performance liquid chromatography mode. Optimized enantiomeric separation conditions were established. Under these conditions, the resolutions of flurbiprofen, naproxen, benzoin, 1,1'-bi-2-naphthol and ketoprofen on the CSP-1 column were 1.61, 2.0, 0.62, 0.52, and 1.20, respectively. In addition, the reproducibility of the CSP-1 column was also investigated. The results of the investigation illustrated that the stationary phases have good reproducibility (RSD = 0.12%, n = 5).
Design of Si0.5Ge0.5 Sourced Nano-cantilever Pressure Sensor Based on Charge Plasma and Gate Stacked Nanowire Tunnel Field Effect Transistor
Authors: Singh Navaneet Kumar 
Published: May 2023

Abstract
In this paper, Si0.5Ge0.5 source with charge plasma and gate stacked Nanowire Tunnel Field Effect Transistor (CP-GS-NWTFET) based pressure sensor is proposed. The pressure sensor is one of the essential components in sensing and actuating applications. Nanocavity is created beneath the gate electrode for possible bending due to the pressure applied. The presented sensor is based on the capacitive gate coupling principle, owing to which the tunneling current is changed. The applied pressure on the nanocantilever (hanging gate electrode towards the cavity) bends the nanocantilever which changes the electrical characteristics of the device. Various bending of the nanocantilever beam used are 0.5, 1, 1.5, and 2nm. Several device parameters including electric field, potential, carrier concentration, energy band diagram, Ion/Ioff ratio, subthreshold slope, etc. are evaluated as performance parameters of the presented device. The sensitivity is defined as the change in the current ratio per nm of bending with respect to a structure having no bending. The study reveals that the presented pressor sensor is more sensitive for lower bendings. The sensitivity for 0.5nm and 2nm of bending is 2.74×1008 /nm and 4.73×1007 /nm respectively. Simulation unearths a remarkable connection between hypothetical and practical accepts of formation. The total length of the proposed device, CP-GS-NWTFET is 92 nm.
Biomechanical Properties of Lumbar Vertebral Ring Apophysis Cage under Endplate Injury: A Finite Element Analysis
Authors: Wang Jian , Geng Ziming , Wu Jincheng , Liu Jianchao , Zhang Zepei , Miao Jun , Li Ruihua 
Published: May 2023

Abstract
Objective
This study aimed to compare the biomechanical properties of lumbar interbody fusion involving two types of cages. The study evaluated the effectiveness of the cage spanning the ring apophysis, regardless of the endplate's integrity.
Methods
A finite element model of the normal spine was established and validated in this study. The validated model was then utilized to simulate Lateral Lumbar Interbody Fusion (LLIF) with posterior pedicle screw fixation without posterior osteotomy. Two models of interbody fusion cage were placed at the L4/5 level, and the destruction of the bony endplate caused by curetting the cartilaginous endplate during surgery was simulated. Four models were established, including Model 1 with an intact endplate and long cage spanning the ring apophysis, Model 2 with endplate decortication and long cage spanning the ring apophysis, Model 3 with an intact endplate and short cage, and Model 4 with endplate decortication and short cage. Analyzed were the ROM of the fixed and adjacent segments, screw rod system stress, interface stress between cage and L5 endplate, trabecular bone stress on the upper surface of L5, and intervertebral disc pressure (IDP) of adjacent segments.
Results
There were no significant differences in ROM and IDP between adjacent segments in each postoperative model. However, the ROM, the pressure of the cage-endplate contact surface, and the stress of the cancellous bone above L5 during the fixation stage tended to increase in the short cage model. This trend was more apparent under the condition of an incomplete endplate. Regardless of the integrity of the endplate, the long cage provided greater support strength compared to the short cage. The corresponding endplate, cancellous stress, and screw rod system stress exhibited a significantly lower trend with the long cage than with the short cage.
Conclusions
Caution should be exercised during endplate preparation and cage placement to maintain the endplate's integrity. The cage spanning the vertebral ring apophysis during lateral lumbar interbody fusion offers favorable biomechanical properties and prevents complications such as cage subsidence, internal fixation system failure, and screw and rod fracture.”
Effect of Regional Disparities on National Economic Growth. A Spatial Panel Data Evidence From Eu Countries in 2000–2020
Authors: Piętak Łukasz 
Published: May 2023

Abstract

The study aims to examine whether regional disparities within a country affect national economic growth. To achieve this, the study employs spatial panel data models that consider the role of geography in economic development. The analysis covers 17 EU countries with five or more regions at the NUTS 2 level, spanning from 2000 to 2020. Regional disparities are calculated using polarization measures. The findings indicate that an increase in regional disparities, in the form of a shrinking range of regions with GDP per capita close to the national average, has a negative impact on economic growth. However, the division of a country's regions into two groups (bipolarization), poor and rich, does not affect national economic performance. Furthermore, the results of robustness tests reveal that regional disparities have a detrimental effect on growth in the EU's new member countries, which joined in 2004 and later, whereas the relationship is insignificant in the case of old member states' economies.
JEL CLASSIFICATION JEL: D30, R11, R58.