bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attenuated adenomatous polyposis with MSH6 variation: two case reports
Authors: Kim Kyoung Min , Lee Min Ro , Ahn Ae Ri , Chung Myoung Ja , Ha Gi Won 
Published: June 2023

Abstract
Background: Adenomatous polyposis (AP) is a genetic disorder characterized by the occurrence of numerous adenomatous polyps in the colon and rectum and can be classified into classical AP (CAP) and attenuated AP (AAP). AAP is diagnosed when the number of observed adenomas is between 10 and 99. The detection of AAP is significantly increasing mainly due to the improvement of the imaging technique and application of the screening program for colorectal cancer detection. Currently, the germline variations of the APC and MUTYH genes are reported as the main cause of CAP. However, the underlying genetic basis of AAP is not well understood. In this study, we report two cases of AAP with MSH6 variations.
Case reports: The two patients had multiple colon polyps and were diagnosed with AAP. The two received genetic consultation; and, for follow-up purposes, both patients agreed to be tested for an underlying genetic condition through next generation sequencing (NGS). Germline MSH6 variations were detected in both patients.
Conclusion: Minor portion of AAP can cause by genetic mutation in MSH6, and further research is needed.
The impact of a health promotion program on toddlers´ socio-emotional development: a cluster randomized study
Authors: Duarte Ana , Martins Silvana , Augusto Cláudia , Silva Maria José , Lopes Luís , Santos Rute , Rosário Rafaela 
Published: June 2023

Abstract
Background
The first three years of life are a critical period for the development of socio-emotional skills, highlighting the importance of socio-emotional development in early childhood. This study aimed to evaluate the effectiveness of a health promotion intervention program on the socio-emotional development of children aged 12 to 42 months during the COVID-19 pandemic.
Methods
A total of 344 children from 15 childcare centers participated in this study, with six centers in the intervention group and nine in the control group. Childcare teachers in the intervention group received a six-month training program aimed at promoting healthy lifestyles, including topics such as diet, sleep, physical activity, and sedentary behavior. Sociodemographic and anthropometric measures were assessed at baseline, and socio-emotional development was assessed using the Bayley Scales of Infant and Toddler Development – Third Edition (Bayley-III) at baseline and post-intervention.
Results
Intervened children whose mothers had a lower education level demonstrated significantly higher socio-emotional development than the controlled children (B = 19.000, p = 0.028), while no significant difference was observed in children with mothers from higher education levels.
Conclusion
These findings suggest that intervention programs for childcare teachers can be effective in promoting healthy socio-emotional development in children from socioeconomically disadvantaged backgrounds. Future intervention programs should consider tailoring their approaches to target disadvantaged populations.
Trial registration
This cluster randomized controlled trial was registered in the Clinical Trials database/platform on 09/09/2019 (number NCT04082247).
Do simulation exercises affect pharmacy students’ self-efficacy in providing pharmaceutical care according to Bandura’s self-efficacy theory? – a pilot study
Authors: Plewka Beata , Waszyk-Nowaczyk Magdalena , Cerbin-Koczorowska Magdalena , Przymuszała Piotr , Osmałek Tomasz 
Published: June 2023

Abstract
Introduction. The transformation of the role of a pharmacist from a drug dispenser to an advisor and patient educator, partially accelerated by COVID-19 pandemic, requires a thorough change in the pharmacy curriculum. Preparation for the provision of modern pharmaceutical services requires the use of the most advanced teaching methods, such as pharmaceutical simulation. Knowledge alone does not guarantee the students’ readiness and motivation to take on new challenges in their professional work, but it seems crucial that graduates of medical faculties have the ability to use their knowledge in practice, also in new and non-standard situations.
Aim of the study. The aim of the study was to assess the impact of simulation exercises on self-efficacy according to the Bandura's theory and self-assessment of the students in the provision of education services for a diabetic patient. Study setting. The questionnaire-based study was conducted during pharmaceutical care peer role-play classes in a simulation environment with a debriefing session among 85 final year pharmacy students at Poznan University of Medical Sciences, Poland. It consisted of two surveys: General Self-Efficacy Scale (GSE) and a pre-post self-assessment and self-efficacy questionnaire.
Results. It was revealed that there is a positive correlation between GSE and self-efficacy (R = 0.52, p < 0.0001). There was also a statistically significant increase in the post-self-assessment of all the skills and competencies included in the survey in the field of pharmaceutical care of a patient with diabetes compared to the pre values (p < 0.001). Also, the students' self-efficacy regarding communication with the patient was higher than before the classes (4.85 vs. 6.26, p < 0.001).
Conclusions. The peer-role play active teaching method turned out to be a cost-effective method that allows to increase the self-assessment and self-efficacy of a pharmacy students in the diabetic patient pharmaceutical care. Further in-depth research is needed to fully confirm the effectiveness of simulation exercises in teaching pharmacy undergraduates.
Differently expressed proteins in serum on elderly patients suffered perioperative neurocognitive disorders underwent transurethral resection of prostate
Authors: Qin Yu-ming , Tang Yi-fan , Liu Ting , Xiao Qiu-Xia , Li Qiu-Yan , Liu Qing , Zhang Ying , Li Hong-Yan 
Published: June 2023

Abstract
Objective: Perioperative neurocognitive impairment (PND) is a common neurological complication after major operation and emergency operation in the elderly. Its pathogenesis still remains unknown. This study aims to explore new targets and prediction methods for PND occurrence.
Methods: 229 elderly patients with prostatic hyperplasia who underwent transurethral resection of prostate(TURP) combined with spinal cord and epidural analgesiawere selected as subjects and divided into PND group and non-PND group according to Z-score method. According to the principle of consistency between preoperative and intraoperative conditions, serum samples of 3 patients were randomly selected from each group. iTRAQ proteomics technology was used to screen the differentially expressed proteins in serum between the two groups. Bioinformatics analysis of the differentially expressed proteins was conducted.
Results: Among 1101 serum proteins of PND group and non-PND group, 8 differentially expressed proteins of PND patients were screened, among which 6 proteins were up-regulated and 2 proteins were down-regulated. Further bioinformatics analysis of differentially expressed proteins showed that they were mainly involved in the biological processes of cells, the formation of cell components, and the biological processes of endocytosis and phagosomes, and had the RING domain of E3 ubiquitin ligase.
Conclusion: iTRAQ proteomics screened the difference of protein molecular expression in serum between PND patients and non-PND patients, and identified 8 differentially expressed proteins. Bioinformatics analysis suggests that differential proteins are mainly involved in the biological processes of microtubules, and the study of neuroplasticity and synaptic formation based on the microtubule formation process may contribute to further understanding and prevention of PND.
Trial registration: Registered(ChiCTR2000028836).Date(20190306).
Role of macrophages in outer membrane of Chronic Subdural Hematoma
Authors: Yang Cheng , Zeng Xiangjun , Yin Jiming , Guo Song , Liu Weiming , Liu Yu 
Published: June 2023

Abstract
Background
Chronic subdural hematoma (CSDH) is a common disease in the field of neurosurgery. Previous studies show that the outer membrane of CSDH is divided into four histological stages by HE staining and elasticavan Gieson staining. However, this method cannot show the proportion of specific inflammatory cell components at different stages. This study aims to explore the major inflammatory cells in the outer membrane of CSDH and discussion their function in CSDH.
Methods
A total of 18 patients were included in this study. A semi-quantitative analysis was performed on the results of Hematoxylin-eosin (HE), immunohistochemistry and Masson staining.
Results
Analysis results show, in stage II, that macrophages make up the highest number of inflammatory cells, followed by T cells, B cells and neutrophil. Eosinophil main express at stage II and stage III. Interestingly, Inflammatory cells in the outer membrane are arranged in this order at any stage. In stage III, the expression number of CD31 is more than in stage II. Masson staining shows that neomembrane fibrosis gradually increases from stage I to stage IV. The expression of Transforming growth factor β(TGF-β) in the outer membrane correlates with the expression of CD68 in neomembrane (R2 = 0.810, P = 0.015).
Conclusion
The external membrane of CSDH may be a process of formation of scarring membrane according to the results of inflammatory elements in the outer membrane. The formation and development of the outer membrane of CSDH changes dynamically, macrophages play an important role in inflammation, neocapillaries and fibrosis, we considered macrophages play a critical role in the development of CSDH.
Transposition of the medial synovial flap to repair the lateral joint capsule in total knee arthroplasty for knee osteoarthritis with permanent patellar dislocation
Authors: Tao Qifeng , Liu Hongwen , Wang Hongping , Chen Chunyu , Lan Yuping , Ming Jie 
Published: June 2023

Abstract

Purpose
To retrospectively review the early- and medium-term clinical outcomes of lateral capsulorrhaphy by medial synovial flap transposition in total knee arthroplasty for the treatment of knee osteoarthritis with permanent dislocation of the patella.
Method
To surgically restore the patellar trajectory in 29 patients with knee osteoarthritis associated with permanent patellar dislocation undergoing total knee arthroplasty using medial synovial flap transposition to repair the lateral joint capsule. Preoperative and 2-year postoperative conditions and knee mobility were compared using the American Knee Society score function score, UCLA activity scale score, and visual analogue (VAS) pain scores. The patients were examined radiographically for full-length knee and axial patellar reduction while standing on both lower limbs.
Result
The patients' American Knee Society function scores improved from 40.2 ± 12.1 preoperatively to 80.5 ± 7.3 at 2 years postoperatively (P < 0.001), and their UCLA activity class scores improved from 3.9 ± 0.6 preoperatively to 6.8 ± 0.7 at 2 years postoperatively (P < 0.001). VAS pain scores decreased from 41.8 ± 6.0 points preoperatively to 10.1 ± 3.7 at two years postoperatively (P < 0.001). Knee mobility improved from 74.6 ± 8.9 degrees preoperatively to 110.5 ± 4.5 degrees at two years postoperatively (P < 0.001), and imaging showed no signs of subluxation or dislocation of the patella in any of the patients.
Conclusions
The correction of the patellar trajectory in patients with osteoarthritis with permanent patellar dislocation undergoing total knee arthroplasty using medial synovial flap transposition to repair the lateral joint capsule can lead to satisfactory early and medium-term clinical results.
Sex-dependent neuronal effects of α-synuclein reveal that GABAergic transmission is neuroprotective of sleep-controlling neurons
Authors: Santos Altair Brito Dos , Thaneshwaran Siganya , Ali Lara Kamal , Leguizamón César Ramón Romero , Wang Yang , Kristensen Morten Pilgaard , Langkilde Annette E. , Kohlmeier Kristi A. 
Published: June 2023

Abstract

Background Sleep disorders (SDs) are a symptom of the prodromal phase of neurodegenerative disorders that are mechanistically linked to the protein α-synuclein (α-syn) including Parkinson’s disease (PD). SDs during the prodromal phase could result from neurodegeneration induced in state-controlling neurons by accumulation of α-syn predominant early in the disease, and consistent with this, we reported the monomeric form of α-syn (monomeric α-syn; α-synM) caused cell death in the laterodorsal tegmental nucleus (LDT), which controls arousal as well as the sleep and wakefulness state. However, we only examined the male LDT, and since sex is considered a risk factor for the development of α-syn-related diseases including prodromal SDs, the possibility exists of sex-based differences in α-synMeffects. Accordingly, we examined the hypothesis that α-synMexerts differential effects on membrane excitability, intracellular calcium, and cell viability in the LDT of females compared to males. Methods Patch clamp electrophysiology, bulk load calcium imaging, and cell death histochemistry were used in LDT brain slices to monitor responses to α-synMand effects of GABA receptor acting agents. Results Consistent with our hypothesis, we found opposite effects of α-synMon female LDT neurons when compared to male. In the females, α-synMinduced a decrease in membrane excitability and reductions in intracellular calcium which were reliant on functional GABAergic receptors, as well as decreased the amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSCs) with a concurrent reduction in action potential firing rate. Cell viability studies showed higher α-syn-mediated neurodegeneration in males compared to females, that depended on inhibitory amino acid transmission. Further, GABA receptor agonists reduced cell death in males. Conclusions When taken together, we conclude that α-synMinduces a sex-dependent effect on LDT neurons involving a GABA receptor-mediated mechanism which is neuroprotective. Understanding the potential sex differences in neurodegenerative processes, especially those occurring early in the disease, could enable implementation of sex-based strategies to identify prodromal PD cases, and promote efforts to illuminate new directions for tailored treatment and management of PD.
Phase II Study of Peg-Asparaginase, Etoposide, Gemcitabine (PEG) Followed by Involved- Field Radiation Therapy in Early-Stage Extranodal Natural Killer/T-Cell Lymphoma
Authors: Wang Hua , feng demei , yan zhimin , Fu Bibo , Bai Shenrui , Zhu Lewei , Gale Robert Peter , Xia Zhongjun , Liang Yang 
Published: June 2023

Abstract
Background The prognosis of extra-nodal NK-/T-cell lymphoma (ENKTL) is poor and the best therapy controversial. We studied safety and efficacy of a new combined modality therapy.Methods Phase-2 study of peg-asparaginase, etoposide and gemcitabine (PEG) and involved field radiation therapy (IFRT) in newly-diagnosed subjects with early-stage ENKTL. 2–6 course of PEG were given depending on response followed by IFRT. The primary endpoint were complete response(CR),partial response(PR),and objective response rate(ORR) after IFRT. Secondary endpoints included progression-free survival (PFS), survival and adverse events.Results 34 consecutive subjects who were Ann Arbor stage-I/-II were enrolled. 3 subjects progressed on PEG, the remaining 31 received IFRT. The ORR was 88.2%(30/34),included 28(82.4%)complete and 2 ༈5.8%༉ partial responses. At a median follow-up of 56.0 months (Interquartile Range [IQR], 36.0-66.9 months),the 5-year PFS and survival were 87.4% (95% Confidence Interval [CI],69.5%-94.8%) and 97.1% (95%CI,80.1%-99.6%). Most adverse events were haematological and easily managed.Conclusions PEG followed by IFRT is a safe and effective initial therapy for low-stage ENKTL, with similar toxicity and response rates, but higher progression-free survival(PFS) and overall survival(OS) rates compared to other regimens, providing an effective and tolerable new regimen for the newly diagnosed early-stage ENKTL.This conclusion needs validation in a randomized controlled trial.
Health Impact Assessment in two Planning Projects in England: Reflections on Normative Effectiveness
Authors: Fischer Thomas B , Chang Michael , Muthoora Tara 
Published: June 2023

Abstract

Background
This article reports on research commissioned by (what was at the time) Public Health England (PHE) on the normative effectiveness of Health Impact Assessment (HIA) applied in two planning situations; (a) a new town at Cranbrook, Devon (HIA prepared in 2007), and (b) the regeneration of the Marsh farm area in Luton (HIA prepared in 2009). The aim was to examine the contribution of HIA to actions that are intended to lead to good or improved health and wellbeing.
Methods
Normative HIA effectiveness criteria were used to guide the analysis. The two included HIA cases were previously identified as good practice examples with regards to procedure and report quality. Semi-structured interviews with public health, planning and other actors originally involved in the HIAs were conducted in 2021. This was followed up by web-searches for evidence on actual developments in 2023.
Results
Interviews indicated that normative effectiveness was initially high, but that the longer term effects of the financial crash of 2008, reduced this. Delays in initially anticipated timelines and HIA actors moving elsewhere meant that HIAs were not followed-up and connections between developments and the HIAs were no longer made. However, key HIA suggestions were eventually implemented, albeit with delay. There is evidence for improved IMD (index of multiple deprivation) rankings in the Marsh farm case.
Conclusion
A mismatch is observed with regards to HIA exercises appearing to be largely ‘forgotten’ after over a decade of their publication, but recommendations still being implemented, possibly as a result of ‘institutional memory’. Making monitoring and follow-up of HIA binding rather than advisory would allow for direct linkages to be made.
Research progress of methods for degradation of Bisphenol A
Authors: Han Ying , Dai Hao , Rong Xiaolong , wang Mingxin , Xue Yingang , Jiang Haixia 
Published: June 2023

Abstract

Bisphenol A (BPA), an endocrine disruptor widely used in industrial production, is found in various environmental sources. Despite numerous reports on BPA degradation and removal, the details remain unclear. This paper aims to address this gap by providing a comprehensive review of BPA degradation methods, focusing on biological, physical, and chemical treatments, and the factors that affect the degradation of BPA. Firstly, the paper uses VOSviewer software to map out the literature on BPA degradation published in the past 20 years, which reveals the trends and research focus in this field. Next, the advantages and limitations of different BPA degradation methods are discussed. Overall, this review highlights the importance of BPA degradation to protect the environment and human health. The paper provides significant insights for researchers and policymakers to develop better approaches for BPA degradation and removal.
Etiologic diagnosis and antimicrobial resistance pattern of nosocomial infection in Pediatric Intensive Care Unit of a Tertiary Hospital, Tigray, Ethiopia
Authors: Mohamed Abdikarin Ahmed , Hadgu Amanuel , Seyoum Dawit , Gebrekidan Goitom , Ebrahim Mohamedawel Mohamedniguss , Mohammedamin Mohammed Mustefa , Gebre Niguse Tsegay , Haftu Hansa 
Published: June 2023

Abstract
Background
Antimicrobial resistance in the pediatric population is emerging and becoming a global threat. Data from the WHO found that infections caused by multidrug-resistant bacteria were found to have a higher death rate across all ages. This study assesses nosocomial infection's etiologic cause and antimicrobial resistance pattern in the Pediatric Intensive Care Unit (PICU).
Result
Forty-five (20.2%) patients admitted to pediatric ICU acquired nosocomial infection (NI). The median age of the patients was one year (IQR 0.5–5.5), with an M: F ratio of 1:1.4. Etiology diagnosis was confirmed in 21 (42.9%) patients. Gram-negative bacteria were the major contributor to NI. Klebsiella pneumonia was the most common isolate, accounting for 34.6%, followed by Acinetobacter (19.2%), Staphylococcus species (11.5%), and Escherichia coli (11.5%). Third-generation cephalosporins (Ceftriaxone, cefotaxime, and ceftazidime) had a 100% resistance against all organisms tested. About 96% of the pathogens had a multidrug-resistant pattern. Methicillin-resistant (MRSA) staphylococcus aureus was also identified in one patient.
Conclusion
The findings of the study revealed that nearly all bacterial isolates demonstrated a multidrug resistance pattern, which is associated with increased morbidity and mortality, despite the delay in administering appropriate therapy. Gram-negative bacteria were the predominant organisms identified.
Understanding the Inconsistent Nature of Blast Resistance in Combined Bacterial Leaf Blight and Blast Gene Pyramided Lines of Rice Variety Tellahamsa
Authors: E Ramaprasad , Ch Durga Rani V , N Neerja C , G Padmavathi , R Jagadeeshwar , C Anjali , Thakur Priya , N Yamini K , S Laha G , Prasad Srinivas M , Alhelaify Seham Sater , Aharthy Ohud Muslat , Sayed Samy M , Mushtaq Muntazir 
Published: June 2023

Abstract

Background
Rice blast and bacterial leaf blight (BLB) are the most limiting factors for rice production in the world which cause yield losses typically ranging from 20 to 30% and can be as high as 50% in some areas of Asia especially India under severe infection conditions.
Methods and Results
An improved line of Tellahamsa TH-625-491 having two BLB resistance genes (xa13 and Xa21) and two blast resistance genes (Pi54 and Pi1) with 95% Tellahamsa genome was used in the present study. TH-625-491 was validated for all four target genes and was used for backcrossing with Tellahamsa. Seventeen IBC1F1 plants heterozygous for all four target genes, 19 IBC1F2 plants homozygous for four, three and two gene combinations and 19 IBC1F2:3 plants also homozygous for four, three and two gene combinations were observed. Among seventeen IBC1F1 plants IBC1F1-62 plant recorded highest recurrent parent genome (97.5%) covering 75 polymorphic markers. A total of 920 IBC1F2 plants were screened and 19 homozygous plants possessing four, three and two target genes along with bacterial blight resistance. Background analysis was done in all 19 homozygous IBC1F2 plants possessing BLB resistance (possessing xa13, Xa21, Pi54 and Pi1 in different combinations) with five parental polymorphic SSR markers. IBC1F2-62-515 recovered 98.5% recurrent parent genome. The four, three and two gene pyramided lines of Tellahamsa exhibited inconsistent resistance to blast.
Conclusions
Thus, there is a dire need for studying the blast pathogen because it is having complicated developmental processes in association with surrounding environments as strategies for survival and dispersal.