bluuebunny/crossref_metadata_2025_split huggingface dataset:
Very High Cycle Fatigue and Ultrasonic Testing in Multiaxial Loading
Authors: Costa Pedro R. , Reis Luís , Freitas Manuel 
Published: August 2023

AbstractFatigue damage has special relevance on the lifespan of mechanical components and structures, as it takes responsibility for the majority of registered structural failures. Most fatigue testing today still uses uniaxial loads, nonetheless it is generally recognized that multiaxial stresses occur in many full-scale structures. Nowadays, the growing need for greater lifespans forced the study of material behavior under what is nowadays very high cycle fatigue (VHCF) regime, fatigue research beyond 10E07 up to 10E10 cycles. However, most classic fatigue machines are often very costly, both in terms of time and energy, for such an elevated number of cycles. The high costs of equipment and time to conduct such experiments have seen a massive improvement with the introduction of high frequency ultrasonic machines. Presently, it is already possible to characterize materials VHCF response under axial and multiaxial loading conditions in a fraction of the time. Ultrasonic testing machines were first introduced for simpler uniaxial loading and more recently adapted for multiaxial loading conditions. The present work reviews the current background of ultrasonic fatigue testing machines working at 20-kHz frequency, with emphasis on multiaxial fatigue and VHCF. A review of a series of fatigue tests are presented, respectively, axial, shear, axial/shear, and in-plane bi-axial fatigue tests with different combining dimension specimens allowing different shear/axial stress ratios. Special attention will be put into the performance of multiaxial classical cylindrical specimens under tension/torsion and flat cruciform specimens under in-plane bi-axial testing using low-cost piezoelectric transducers. By comparing all so far developed and successfully reached multiaxial specimens and respective methodologies, two base approaches were possible to be established. When designing new specimens or ultrasonic machines, or both, one of the two methods must be followed. To have a well-comprehended methodology and successful design approach will undoubtedly guide future multiaxial ultrasonic method ideas.
Disseminated cysticercosis in humans
Authors: Celi Simbaña Stalin Santiago , Andrade Mora Diego Sebastián 
Published: August 2023

AbstractHuman cysticercosis, caused by the parasitic infection of Taenia Solium, is an important challenge for public health in Ecuador and other countries where poor sanitary conditions are a key factor for its appearance. In this series of cases, we publish the imaging findings corresponding to three patients treated in 2022 at the Center of Medical Specialties “Comité del Pueblo” Quito - Ecuador, in which, through radiographic images, multiple radiodense lesions were found in different anatomical regions which follow the typical pattern of distribution parallel to the muscular tissues of the affected region, generating the radiological sign known as “rice grain calcifications”, which corresponds to the presence of Disseminated Cysticercosis (DCC)in the muscular tissue of the host.
WITHDRAWN: Hierarchical Attentive Feature Aggregation for Person Re-identification
Authors: Dong Husheng , Lu Ping 
Published: August 2023

Abstract
Recent efforts on person re-identification have shown promising results by learning discriminative features via multi-branch network. To further boost feature discrimination, attention mechanism has also been extensively employed. However, the branches in existing models generally work independently, which may compromise the ability of mining diverse features. To mitigate this issue, a novel framework called Hierarchical Attentive Feature Aggregation (Hi-AFA) is proposed. In Hi-AFA, a hierarchical aggregation mechanism is applied to learn attentive features. The current feature map is not only fed into the next stage, but also aggerated into another branch, leading to hierarchical feature flows along depth and parallel branches. We also present a simple feature suppression operation and a lightweight dual attention module with only a few parameters to guide feature learning. By this manner, the branches could cooperate to mine richer and more diverse feature representations. We integrate the hierarchical aggregation and multi-granularity feature learning into a unified architecture that builds upon OSNet, resulting a resource-economical and effective person re-identification model. Extensive experiments on three public datasets, including Market-1501, DukeMTMC-reID, and CUHK03, are conducted to validate the effectiveness of the proposed method, and results show that state-of-the-art performance is achieved.
Rhizobium zhangyense sp. nov., a novel species isolated from a root nodule of Hedysarum multijugum in China
Authors: Xu Lin , Gao Guoji , Wan Liang , Friman Ville Petri , Liu zhenshan 
Published: August 2023

Abstract
Purpose
Rhizobia play an important ecological and agricultural role in the N2 cycle between atmosphere and biosphere on the earth. The taxonomy of rhizobia is in a state of flux due to discovery of many novel species, which has led to numbers of revisions in the species description.
Results
A Gram-stain-negative mobile strain, designated as a22-2T, was isolated from a nodule of Hedysarum multijugum grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence, recA, glnA and atpD showed that the strain was closely related to genus Rhizobium with sequence similarities less than 98.4% (16S rRNA), 85.3% (recA), 90.9% (glnA) and 54.2% (atpD). The strain a22-2T could grow at temperatures between 20-40°C (optimum, 28°C) and at pH between 5.0-10.0 (optimum, pH 7.0). The cells contained ubiquinone Q-10 as the sole respiratory quinone and had 18:1 ω7c (43.8%) as the major cellular fatty acid. The DNA relatedness with the most closely related reference strains was below 48%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, the strain a22-2T represents a novel species of the genus Rhizobium, for which the name Rhizobium zhangyense sp. nov. is proposed. The type strain of Rhizobium zhangyense sp. nov. is a22-2T (= CGMCC 1.15517T = NBRC 112336T). The genome sequence of the isolate was determined. It’s G+C content was 61.74%, and the 85% average nucleotide identity of the type strain genome to its best neighbour with a completely sequenced genome (Rhizobium selenitireducens ATCC BAA-1503) indicated low genomic relatedness.
Association between COL6A4P1 gene polymorphisms and knee osteoarthritis in the North Chinese Han population
Authors: Luo Shengfei , Zhang Dejian , Yin Haoran , Zhao Lixi , Liu Xiaomeng 
Published: August 2023

Abstract

Objective
Previous studies have shown that knee arthritis is a disease influenced by environmental and genetic factors. In this paper, we mainly investigate the association between SNPs in the COL6A4P1 gene region and the susceptibility to KOA in north Chinese Han people.
Methods
We performed two-center cross-sectional observational study that included 103 Chinese Han patients with KOA and 128 healthy Chinese Han volunteers. We investigated four SNPs (rs11718863, rs7639618, rs7651842 and rs7639807) in the COL6A4P1 gene region and extracted the genes using QIAamp DNA Mini Kit. We amplified the target gene fragment and sequenced the genotype. The corresponding frequency were counted and the counting results were statistically analyzed.
Results
The patient group was significantly older than the control group, and the difference was statistically significant(P < 0.001). There were no statistically significance between-group differences in gender, height, weight, or BMI (P > 0.05, respectively). The chi-square test was used to analyze the four SNPs of COL6A4P1 gene, and rs11718863 and rs7639618 polymorphism was statistically different between the two groups(P = 0.012, P = 0.023, respectively). The rs11718863 and rs7639618 was consistent with Hardy-Weinberg equilibrium. Logistic regression analysis showed that KOA risk was significantly increased in the rs11718863 TT genotype (2.926, 95%CI 1.410, 6.069, P = 0.004) and the rs7639618 TT genotype (OR 3.50, 95% CI 1.687, 7.260, P = 0.001). After age adjustment, the same result was observed in the rs11718863 SNP(OR 2.649, 95% CI 1.251, 5.611, P = 0.011), and the risk of KOA disappeared in the rs7639618 SNP (OR 3.304, 95% CI 1.562, 6.990, P = 0.002). The rs7651842 and rs7639807SNP were monomorphic for the T and C allele respectively.
Conclusions
We found that the rs11718863 and rs7639618 SNP were associated with KOA. The TT genotype and T allele were the highest risk factors for the development of KOA in the north Chinese Han population.
Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study
Authors: Bisschoff Michelle , Smuts Izelle , Dercksen Marli , Schoonen Maryke , Vorster Barend Christiaan , Watt George van der , Spencer Careni , Naidu Kireshnee , Henning Franclo , Meldau Surita , McFarland Robert , Taylor Robert , Patel Krutik , Fassad Mahmoud R , Vandrovcova Jana , Consortium The ICGNMD , Wanders Ronald JA , Westhuizen Francois Hendrikus van der 
Published: August 2023

Abstract
Background
Multiple acyl-CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder resulting from pathogenic variants in three distinct genes, with most of the variants occurring in ETFDH. Recent evidence of potential founder variants for MADD in the South African (SA) population, initiated this extensive investigation. As part of the ICGNMD study, we recruited a cohort of patients diagnosed with MADD from academic medical centres across SA over a three-year period. The aim was to extensively profile the clinical, biochemical, and genomic characteristics of MADD in this understudied population.
Methods
Clinical evaluations and whole-exome sequencing were conducted on each patient. Metabolic profiling was performed before and after treatment, where possible. Lastly, segregation analyses and, for the two main variants, haplotype and allele frequencies were determined in the four largest SA populations.
Results
Twelve unrelated families (ten of White SA and two of mixed ancestry) with clinically heterogeneous presentations in 14 affected individuals were observed, and five pathogenic ETFDH variants were identified. Based on disease severity and treatment response, three distinct groups emerged. The most severe and fatal presentations were associated with the c.[1067G > A] and c.[1067G > A];c.[976G > C] genotypes, causing types I and I/II MADD, respectively. These, along with three less severe genotypes, c.[1067G > A];c.[1448C > T], c.[740G > T];c.[1448C > T], and c.[287dup*];c.[1448C > T]), resulting in types II/III MADD, presented before the age of five years, depending on time and maintenance of intervention. By contrast, the c.[1448C > T] genotype, that causes type III MADD, presented later in life. Except for the type I, I/II and II cases, urinary metabolic markers for MADD improved/normalised following treatment with riboflavin and L-carnitine. Furthermore, genetic analyses of the most frequent variants (c.[1067G > A] and c.[1448C > T]) revealed a shared haplotype in the region of ETFDH, with South African population-specific allele frequencies of < 0.00067–0.00084%.
Conclusions
This study reveals the first extensive MADD genotype–phenotype profile in the diverse and understudied SA population, in which MADD is most prevalent in the White population. Altogether, this study provides the data required to support early screening followed by genetic counselling, and patient-specific treatment of MADD in South Africa.
Use of Machine Learning-Based Combined Nomogram Model to Predict Autism Spectrum Disorder (ASD) Screening Needs of Children: A Retrospective Cohort Study of 193,101 Children in the US
Authors: Lee Yu-Sheng , Sprong Matthew Evan , Yang Yingyuan , Shrestha Junu , Smeltzer Matthew P. 
Published: August 2023

Abstract
Background: Autism Spectrum Disorder (ASD) has continued to increase in incidence rates in the past two decades. The purpose of the current study was to develop nomograms to assist in the prediction and identification of children with possible ASD diagnoses so that early interventions can be deployed to improve overall long-term functioning.
Methods: Two model selection methods, least absolute shrinkage and selection operator (LASSO) and logistic regression, were used to develop the nomograms to predict ASD based on a large cohort from the National Survey of Children’s Health (NSCH), which included 193,101 children across all 50 states between 2016 and 2021, in the US. We used the area under the curve (AUC) to verify the discrimination performance. The sensitivity and specificity were also estimated by the optimal cutoff through Youden's J Index, Concordance Probability, and Minimize Euclidean distance.
Results: LASSO and logistic regression models selected common predictors for ASD, including age, sex, whether born premature, BMI, brain injury, cerebral palsy diagnosis, anxiety, depression, having behavior or conduct problems, developmental delay, having speech or language disorders, learning disability, deafness or hearing problem, having difficulty in concentrating or remembering, having difficulty in making or keeping friends, and ever lived with anyone who had alcohol or drug problems. Maternal age at delivery and Attention Deficit Hyperactivity Disorder (ADHD) were predictors for ASD selected by the LASSO model. On the contrary, the logistic method determined asthma for the ASD predictor, which was not selected by the LASSO regression. Both LASSO and logistic methods showed excellent discrimination with 0.95 AUC. The correct classification rate, optimal sensitivity, and optimal specificity were 89.2% to 89.8%, 86.3 to 86.4%, and 89.3 to 89.9%, respectively.
Conclusions: Based on two well-validated predictor models, we built the nomograms for the early prediction of ASD children. Our easy-to-use nomograms are not intended to replace the standard diagnostic methods; instead, it was used to help parents, clinicians, and researchers better estimate children’s potential risk of ASD by comparing the results of the two models.
Characterizing attitudes related to future child-bearing in young women diagnosed with early-stage breast cancer
Authors: Umashankar Saumya , Li Moming , Blevins Kaylee , Kim Mi-Ok , Majure Melanie , Park John , Huppert Laura A. , Melisko Michelle , Rugo Hope S. , Esserman Laura , Chien A. Jo 
Published: August 2023

Abstract
Purpose
This study characterizes attitudes and decision-making around the desire for future children in young women newly diagnosed with early-stage breast cancer and assesses how clinical factors and perceived risk may impact these attitudes.
Methods
This is a prospective study in women <45years with newly diagnosed stage 1-3 breast cancer. Patients completed a REDCap survey on fertility and family-building in the setting of hypothetical risk scenarios. Patient, tumor, and treatment characteristics were collected through surveys and medical record.
Results
Of 140 study patients (median age=41.4[range 23-45]), 71(50.7%) were interested in having children. Women interested in future childbearing were younger than those who were not interested (mean=35.2[SD=5.2] vs 40.9years[3.90], respectively, p<0.001), and more likely to be childless (81%vs31%, p<0.001). 54 women (77.1% of patients interested in future children) underwent/planned to undergo oocyte/embryo cryopreservation before chemotherapy. Interest in future childbearing decreased with increasing hypothetical recurrence risk, however 17% of patients wanted to have children despite a 75-100% hypothetical recurrence risk. 24.3% of patients wanted to conceive <2years from diagnosis, and 35% of patients with hormone receptor positive tumors were not willing to complete 5 years of hormone therapy.
Conclusion
Many young women diagnosed with early-stage breast cancer prioritize childbearing. Interest in having a biologic child was not associated with standard prognostic risk factors. Interest decreased with increasing hypothetical recurrence risk, though some patients remained committed to future childbearing despite near certain hypothetical risk. Individual risk assessment should be included in family-planning discussions throughout the continuum of care as it can influence decision-making.
A novel approach to designing viral precision vaccines applied to SARS-CoV-2
Authors: TRABELSI Khaled , KHALAF Noureddine BEN , RAMADAN Ahmed , CHLIF Sadok , BOUSSOFFARA Thouraya , Ahmed Melika Ben , Fathallah M-Dahmani , Ashoor Dana 
Published: August 2023

Abstract
Efficient precision vaccines against several highly pathogenic zoonotic viruses are currently lacking. Here, we describe a new approach to developing viral precision vaccines based on specific antigen selection through its application to the design of a SARS-CoV-2 cross-variant vaccine. This approach targets viral structural regions involved in infectivity to identify conserved immunogenic epitopes and select those eliciting specific immune responses in infected people. An important step of this approach consists of engineering vaccinating antigens that carry the selected epitopes and mimic their 3D native structure. In our study, the epitopes selected from SARS-CoV-2 revealed an antibody response significantly associated with mild and asymptomatic COVID-19, which strongly suggests protective immunity. Engineered antigens containing the SARS-CoV-2 selected epitopes generated a robust antibody response in mice. Limitations include the need for animal challenge with live virus. Taken together, our results show the potential of this approach for designing precision viral vaccines.
Identification of two novel QTL for Fusarium head blight resistance in German wheat cultivar Centrum
Authors: Ren Hui , Zhang Xin , Zhang Yibo , Zhang Lu , Zhang Xiangxue , Li Chunchun , Duan Jiangli , Zhang Ziyang , Cheng Muzi , Xiang Mingjie , Liu Shengjie , Zeng Qingdong , Wu Jianhui , Kang Zhensheng , Li Chunlian , Huang Shuo , Han Dejun 
Published: August 2023

Abstract

Fusarium head blight (FHB) is a devastating disease that occurs in warm and humid environments. The German winter wheat cv. Centrum has displayed moderate to high levels of FHB resistance in the field for many years. In this study, a F6:8 recombinant inbred line (RILs) population developed from cross Centrum × Xinong 979 was evaluates for FHB resistance following point inoculation in five environments. The population and parents were genotyped using the GenoBaits Wheat 16K Panel. Two stable QTL associated with FHB resistance in Centrum were mapped on chromosome arms 2DS and 5BS, respectively. The more effective QTL on 2DS, identified as a new locus, was identified in each environment, and was mapped to a 1.4 Mb interval that included 17 candidate genes by using resequencing data. The novel QTL on 5BS mapped to the centromere region of a reciprocal translocation 5BS-7BS chromosome. In addition, two environmentally sensitive QTL were mapped on chromosome arms 2BL from Centrum and 5AS from Xinong 979. Polymorphisms of flanking AQP markers AQP-6 and AQP-22 for the QTL on 2DS were validated in a panel of 217 cultivars and breeding lines. These two markers could be useful for marker-assisted selection of FHB resistance and also provide a starting point for fine mapping and marker-based cloning of the resistance gene.
Osmotic stress-induced CsRCI2E endosomal trafficking promotes the redistribution of aquaporin CsPIP2 at the plasma membrane of Camelina sativa L.
Authors: Shin Jung-Ho , Kim Hyun-Sung , Kim Sehee , Ahn Sung-Ju 
Published: August 2023

Abstract
Rare Cold Inducible 2s (RCI2s) are proteolipids that travel from the plasma membrane (PM) to the endomembrane. The expression of RCI2s is induced by abiotic stresses such as cold, heat, drought, and salinity and affects abiotic stress tolerance in plants. It has been reported that CsRCI2E interacts with the water transport protein CsPIP2;1 to reduce its abundance at the PM during NaCl stress. Therefore, CsRCI2E is considered a potential factor affecting the endocytosis of CsPIP2s for maintaining cell homeostasis; however, its exact function in membrane trafficking remains unclear. In this study, we observed the rapid internalization of CsRCI2E and CsPIP2s under both mannitol- and NaCl-induced osmotic stresses using a sucrose density gradient. The transcription of CsRCI2Eincreased markedly 3 h after treatment with mannitol or NaCl. The over-expression of CsRCI2E enhanced stress tolerance and reduced cell damage from reactive oxygen species accumulation in germinating Camelina. Interestingly, the subcellular distribution of CsRCI2E and CsPIP2s shifted rapidly from the PM to the endomembrane within 0.5 h after both osmotic shocks even though the CsRCI2E gene expression had not been changed by the stresses at that time. Additionally, CsRCI2Eoverexpression caused the internalization and subcellular redistribution of CsRCI2E and CsPIP2 under osmotic stress conditions as well as no stress conditions. These results suggest that the internalization of CsRCI2E is a sensing mechanism in the early stages of osmotic shock. Furthermore, an increased amount of CsRCI2E promotes the membrane trafficking of CsPIP2 from the PM to the endomembrane to maintain water homeostasis in Camelina.
Ocular Features of Pseudoxanthoma Elasticum Observed by Multispectral Imaging
Authors: Ma Feiyan , Wang Hong , Yuan Jing , Guo Congrong , Kozak Igor 
Published: August 2023

Abstract
Clinically there are controversies on the nature of peau d'orange change in pseudoxanthoma elasticum (PXE). It was originally described as pigmentary changes due to the dark spots under funduscopic examination, but later other features by infra-red image, autofluorescence and spectral-domain OCT indicated the lack of pigment clumping activity in the outer retina. We recently developed light-emitting-diode-based multispectral imaging system for investigation of ocular pigment changes and, in this study, we enrolled PXE chorioretinopathy as a pilot case to demonstrate its ability of revealing spectrum-sensitive imaging features of MSI.
Isolation and functional characterization of cold-induced gene (AmCIP) promoter from Ammopiptanthus mongolicus
Authors: Ge Bohao , Dong Kuo , Li Rongchen , Bi Xiaorui , Liu Qianru , Zhang Weiwei , Chen Yuzhen , Lu Cunfu 
Published: August 2023

Abstract

AmCIP is a dehydrin-like protein which involved in abiotic stress tolerance in xerophytes evergreen woody plant A. mongolicus. AmCIP could be induced in the cotyledon and radicle during cold acclimation. To further elucidate the regulation of the upstream region of the gene, we isolated and characterized the promoter of AmCIP. Herein, a 1048 bp 5’-flanking region of AmCIP genomic DNA was isolated and cloned by Genome walking from A. mongolicus and the segment sequence was identified as “PrAmCIP” promoter. Analysis of the promoter sequence revealed the presences of some basic cis-acting elements, which were related to various environmental stresses and plant hormones. GUS histochemical staining showed that PrAmCIP was induced by 4℃, 55℃, NaCl, drought and ABA, whereas it could hardly drive GUS gene expression under normal conditions. Furthermore, we constructed three deletion fragments and genetically transformed them into Arabidopsis thaliana. GUS histochemical staining showed that the MYCATERD1 element of the CP7 fragment(-122~-1)may be a key element in response to drought. In conclusion, we provide an inducible promoter, PrAmCIP, which can be applied to the development of transgenic plants under abiotic stresses.