bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preferences reveal separable valuation systems in prefrontal-limbic circuits
Authors: Stoll Frederic M. , Rudebeck Peter H. 
Published: May 2023

ABSTRACTIndividual preferences for the flavor of different foods and fluids exert a strong influence on behavior. Most current theories posit that preferences are integrated with other state variables in orbitofrontal cortex (OFC), which is thought to derive the relative subjective value of available options to drive choice behavior. Here we report that instead of a single integrated valuation system in OFC, another separate one is centered in ventrolateral prefrontal cortex (vlPFC) in macaque monkeys. Specifically, we found that OFC and vlPFC preferentially represent outcome flavor and outcome probability, respectively, and that preferences are separately integrated into these two aspects of subjective valuation. In addition, vlPFC, but not OFC, represented the outcome probability for the two options separately, with the difference between these representations reflecting the degree of preference. Thus, there are at least two separable valuation systems that work in concert to guide choices and that both are biased by preferences.
Ketosis regulates K+ion channels, strengthening brain-wide signaling disrupted by age
Authors: van Nieuwenhuizen Helena , Chesebro Anthony G. , Polizu Claire , Clarke Kieran , Strey Helmut H. , Weistuch Corey , Mujica-Parodi Lilianne R. 
Published: May 2023

AbstractAging is associated with impaired signaling between brain regions when measured using resting-state fMRI. This age-related destabilization and desynchronization of brain networks reverses itself when the brain switches from metabolizing glucose to ketones. Here, we probe the mechanistic basis for these effects. First, we established their neuronal basis using two datasets acquired from resting-state EEG (Lifespan:standard diet, 20-80 years, N = 201;Metabolic:individually weight-dosed and calorically-matched glucose and ketone ester challenge,μage= 26.9 ± 11.2 years, N = 36). Then, using the multi-scale Larter-Breakspear neural mass model, we identified the unique set of mechanistic parameters consistent with our clinical data. Together, our results implicate potassium (K+) gradient dysregulation as a mechanism for age-related neural desynchronization and its reversal with ketosis, the latter finding of which is consistent with direct measurement of ion channels.
Bayesian updating for self-assessment explains social dominance and winner-loser effects
Authors: Perkes Ammon , Laskowski Kate 
Published: May 2023

AbstractIn animal contests, winners of previous contests often keep winning and losers keep losing. This coupling of previous experiences to future success, referred to as the winner-loser effect, plays a key role in stabilizing the resulting dominance hierarchies. Despite their importance, the cognitive mechanisms through which these effects occur are unknown. Identifying the mechanisms behind winner-loser effects requires identifying plausible models and generating predictions that can be used to test these alternative hypotheses. Winner-loser effects are often accompanied by a change in the aggressiveness of experienced individuals, which suggests individuals may be adjusting their self-assessment of their abilities after each contest. This updating of a prior estimate can be effectively described by Bayesian updating, and here we implement an agent-based model with continuous Bayesian updating to explore whether this is a plausible explanation of winner-loser effects. We first show that Bayesian updating reproduces known empirical results of typical dominance interactions. We then provide a series of testable predictions that can be used in future empirical work to distinguish Bayesian updating from simpler mechanisms. Our work demonstrates the utility of Bayesian updating as a mechanism to explain and ultimately predict changes in behaviour after salient social experiences.
The Non-Phototrophic Hypocotyl3 (NPH3)-domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance
Authors: Upadhyay-Tiwari Neha , Huang Xin-Jie , Lee Yi-Chen , Singh Shashi Kant , Hsu Chuan-Chi , Huang Shih-Shan , Verslues Paul E. 
Published: May 2023

AbstractThe mechanisms of plant resistance to low water potential (ψw) during drought are unclear but may involve signaling and trafficking at the plasma membrane as well as metabolic reprogramming, including proline accumulation. Forward genetic screening using aProline Dehydrogenase 1(ProDH1) promoter:reporterline identified a mutant with extreme low ψwhypersensitivity due to a single amino acid substitution (P335L) in the Non-Phototrophic Hypocotyl3 (NPH3) domain of NPH3/RPT2-Like5 (NRL5)/Naked Pins in Yucca8 (NPY8). Further experiments found that NRL5, and other NPH3-domain proteins, are GTPases. NRL5 interacted with RAB small GTPases and the SNARE proteins VAMP721/722 and had polar localization. NRL5P335Lhad greatly reduced GTPase activity, impaired RAB and VAMP721/722 interaction and disrupted polar localization. These data demonstrate that NRL5-mediated restraint of proline catabolism is required for drought resistance and also more broadly define unexpected functions of the NPH3 domain such that the role of NPH3-domain proteins in signaling, trafficking, and cellular polarity can be critically re-evaluated.One-Sentence SummaryA protein containing the plant-specific NPH3-domain has GTPase activity, trafficking interaction and drought resistance function.
Copine-6 is a Ca2+sensor for activity-induced AMPA receptor exocytosis
Authors: Anson Tan Jing Zhi , Jang Se Eun , Batallas-Borja Ana , Bhembre Nishita , Chandra Mintu , Zhang Lingrui , Guo Huimin , Ringuet Mitchell T. , Widagdo Jocelyn , Collins Brett M. , Anggono Victor 
Published: May 2023

SUMMARYThe recruitment of synaptic AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors underlies the strengthening of neuronal connectivity during learning and memory. This process is triggered by NMDA (N-methyl-D-aspartate) receptor-dependent postsynaptic Ca2+influx. Synaptotagmin (Syt)-1 and −7 have been proposed as Ca2+-sensors for AMPA receptor exocytosis, but are functionally redundant. Here we identify a cytosolic C2 domain-containing Ca2+-binding protein Copine-6 that forms a complex with AMPA receptors. Loss of Copine-6 expression impairs activity-induced exocytosis of AMPA receptors in primary hippocampal neurons, which is rescued by wild-type Copine-6, but not Ca2+-binding mutants. In contrast, Copine-6 loss-of-function has no effects on steady-state expression or tetrodotoxin-induced synaptic upscaling of surface AMPA receptors. Loss of Syt-1/-7 significantly reduces Copine-6 protein expression. Interestingly, overexpression of wild-type Copine-6, but not the Ca2+-binding mutant, restores activity-dependent exocytosis of AMPA receptors in Syt-1/-7 double-knockdown neurons. We conclude that Copine-6 is a postsynaptic Ca2+-sensor that mediates AMPA receptor exocytosis during synaptic potentiation.
Genome-to-genome analysis reveals associations between human and mycobacterial genetic variation in tuberculosis patients from Tanzania
Authors: Xu Zhi Ming , Zwyer Michaela , Brites Daniela , Hiza Hellen , Sasamalo Mohamed , Reinhard Miriam , Doetsch Anna , Borrell Sonia , Naret Olivier , Rüeger Sina , Lawless Dylan , Isihaka Faima , Temba Hosiana , Maroa Thomas , Naftari Rastard , Beisel Christian , Hella Jerry , Reither Klaus , Portevin Damien , Gagneux Sebastien , Fellay Jacques 
Published: May 2023

AbstractThe risk and prognosis of tuberculosis (TB) are affected by both human and bacterial genetic factors. To identify interacting human and bacterial genetic loci, we leveraged paired human andMycobacterium tuberculosis(M.tb) genomic data from 1000 Tanzanian TB patients. Through a genome-to-genome approach, we identified two pairs of human andM.tbgenetic variants that are significantly associated. One of the human genetic variants maps to the intron ofPRDM15, a gene involved in apoptosis regulation. The other human variant maps to an intergenic region close toTIMM21andFBXO15. In addition, we observed that a group of linkedM.tbepitope variants were significantly associated with HLA-DRB1 variation. This suggests that even though epitope variation is rare inM.tbin general, specific epitopes might still be under immune selective pressure. Overall, our study pinpoints sites of genomic conflicts between humans andM.tb, suggesting bacterial escape from host selection pressure.
Vitamin D deficiency increases vulnerability to canagliflozin-induced adverse effects on 1,25-dihydroxyvitamin D and PTH
Authors: Shahidzadeh Yazdi Zhinous , Streeten Elizabeth A. , Whitlatch Hilary B. , Montasser May E. , Beitelshees Amber L. , Taylor Simeon I. 
Published: May 2023

AbstractContext.Canagliflozin has been reported to increase the risk of bone fracture – possibly mediated by decreasing 1,25-dihydroxyvitamin D [1,25(OH)2D] and increasing PTH.Objective.To investigate whether baseline vitamin D (VitD) deficiency renders individuals vulnerable to this adverse effect and whether VitD3 supplementation is protective.Design.This study had a paired design comparing individual participants before and after VitD3 supplementation.Setting.Community-based outpatient.Patients.11 VitD deficient (25-hydroxyvitamin D [25(OH)D] ≤ 20 ng/mL) individuals recruited from the Amish population in Lancaster PA.Interventions.Participants underwent two canagliflozin challenge protocols (300 mg daily for five days): the first before and the second after VitD3 supplementation. In the VitD3 supplementation protocol, participants received VitD3 supplementation (50,000 IU once or twice a week depending on BMI for 4-6 weeks) to achieve 25(OH)D ≥ 30 ng/mL.Main Outcome Measures.Two co-primary endpoints were identified: effects of VitD3 supplementation on canagliflozin-induced changes in 1,25(OH)2D and PTH. Secondary endpoints included effects of VitD3 supplementation on baseline levels of VitD metabolites and PTH.Results.VitD3 supplementation increased mean 25(OH)D from 16.5±1.6 to 44.3±5.5 ng/mL (p=0.0006) and 24,25-dihydroxyvitamin D [24,25(OH)2D] from 1.0±0.1 to 4.3±0.6 ng/mL (p=0.0002). Mean 1,25(OH)2D and PTH were unchanged. VitD3 supplementation decreased the magnitude of canagliflozin-induced changes in 1,25(OH)2D (from −31.3%±4.7% to −9.3%±8.3%; p=0.04) and PTH (from +36.2%±6.2% to +9.7%±3.7%; p=0.005).Conclusions.VitD deficiency rendered individuals more vulnerable to adverse effects of canagliflozin on biomarkers associated with bone health. VitD3 supplementation was protective against canagliflozin’s short-term adverse effects on 1,25(OH)2D and PTH.
Straining the root on and off triggers local calcium signaling
Authors: Audemar Vassanti , Guerringue Yannick , Frederick Joni , Melogno Isaty , Vinet Pauline , Babataheri Avin , Legué Valérie , Thomine Sébastien , Frachisse Jean-Marie 
Published: May 2023

AbstractThroughout their life, plant root are submitted to mechanical stresses due to pressure exerted by the soil. So far, few studies addressed root cell deformation and calcium signaling elicited by soil compression. In this study, we designed a microchip inspired by pneumatic microvalve concept in order to deliver a lateral pressure to the root of a plant expressing the RGECO1-mTurquoise calcium reporter. Lateral pressure applied on the root induced a moderate elastic deformation of root cortical cells and elicited a multicomponent calcium signal at the onset of the pressure pulse, followed by a second one at the release of the pressure. This indicates that straining rather than stressing of tissues is relevant to trigger the calcium signal. The calcium elevation was restricted to the tissue under pressure and did not propagate. Additionally, the calcium signals exhibited a remarkable attenuation upon repetitive stimulations.Highlights-A microvalve concept mimicking lateral soil pressure was developed.-Non-damaging lateral compression of the root induces an elastic deformation of cortical cells.-A multicomponent calcium signal is elicited at the onset of a pressure pulse and upon release of the pressure.-Straining rather than stressing of tissues is relevant to trigger the calcium signal.-The calcium signal is localized at the tissue under pressure and does not propagate.-Calcium signals exhibit a remarkable attenuation upon repetitive stimulations.
DDHD2 interacts with STXBP1 to mediate long-term memory via the generation of myristic acid
Authors: Akefe Isaac O. , Matthews Benjamin , Saber Saber H. , Venkatesh Bharat G. , Gormal Rachel S. , Blackmore Daniel G. , Sieriecki Emma , Gambin Yann , Bertran-Gonzalez Jesus , Michaels Alysee A. , Xue Mingshan , Cravatt Benjamin , Joensuu Merja , Wallis Tristan P. , Meunier Frédéric A. 
Published: May 2023

AbstractThe phospholipid and free fatty acid (FFA) composition of neuronal membranes plays a crucial role in learning and memory, but the mechanisms through which neuronal activity affects the brain’s lipid landscape remain largely unexplored. Saturated FFAs, particularly myristic acid (C14:0), strongly increase during neuronal stimulation and memory acquisition, suggesting the involvement of phospholipase A1 (PLA1) activity in synaptic plasticity. Here, we show that genetic ablation of the DDHD2 isoform of PLA1 in mice reduced memory performance in reward-based learning and spatial memory models prior to the development of neuromuscular deficits, and markedly reduced saturated FFAs across the brain. DDHD2 was shown to bind to the key synaptic protein STXBP1. Using STXBP1/2 knockout neurosecretory cells and a haploinsufficientSTXBP1+/-mouse model of STXBP1 encephalopathy that is also associated with intellectual disability and motor dysfunction, we show that STXBP1 controls the targeting of DDHD2 to the plasma membrane and the generation of saturated FFAs in the brain. Our findings suggest key roles for DDHD2 and STXBP1 in the lipid metabolism underlying synaptic plasticity, learning and memory.
Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor
Authors: DeBouver Nicholas D. , Bolejack Madison J. , Esan Taiwo E. , Krysan Damian J. , Hagen Timothy J. , Abendroth Jan 
Published: May 2023

The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.
Sustainability risk assessment in mega construction projects
Authors: Coskun Cansu , Dikmen Irem , Birgonul M. Talat 
Published: May 2023

PurposeMegaprojects are large-scale and long-term investments. Three pillars of sustainable construction objectives, namely, social, environmental and economic, should be integrated into megaproject risk management to ensure long-term success. A risk assessment method, Risk Assessment Method for Sustainable Construction Objectives in Megaprojects (RAMSCOM), was developed for this purpose.Design/methodology/approachRAMSCOM was developed based on the latest concepts and identifying relevant and critical sustainability objectives and risks through an extensive literature review. Analytical Hierarchy Process (AHP) and Cross-Impact Analysis (CIA) were used to determine and quantify the threats regarding the importance of the sustainability objectives. The applicability of RAMSCOM was demonstrated on a real megaproject.FindingsThe findings revealed that sustainability risk assessment requires integration of economic, environmental, social objectives and analysis of cross-impacts of risk factors. Visualization of interrelated threats, vulnerabilities and objectives has a potential to support risk mitigation decisions to achieve sustainability goals.Research limitations/implicationsThe method has been developed based on the findings from a detailed literature survey on sustainability objectives and risks. RAMSCOM was tested on a single project with the assistance of three experts' views. Findings from the case project cannot be directly generalised for various megaprojects considering the unique nature of megaprojects.Practical implicationsDecision-makers can use RAMSCOM to assess sustainability risks in megaprojects and develop risk management plans for the most vulnerable and important sustainable objectives in a visual and quantified approach to ensure megaproject's sustainability in the long-term.Originality/valueThe theoretical contribution is a novel risk assessment method that integrates all dimensions of sustainability and quantifies the vulnerability of sustainability objectives considering their priorities, interrelations and risks. Sustainability dimensions, objectives and risks used in RAMSCOM can be useful for other researchers aiming to develop similar methods.
Energy performance assessment of venetian blinds in south-oriented residential spaces in hot arid desert climates
Authors: El-Deeb Khaled 
Published: May 2023

Purpose
Window shading has always been an effective technique to control the access of solar radiation; however, inappropriate selection of the shading technique, location and optical properties may lead to an increase in energy consumed for cooling and artificial lighting. Venetian blinds (VBs) are a type of adjustable shading devices that can be installed to the interior, exterior or in between glass panes of a window and that can be easily implemented in both new and existing buildings. This study aims to investigate the impact of three VB parameters: slat angle, reflectivity and location on the overall energy consumption of a residential space with a south-facing facade under the hot arid desert climate of Saudi Arabia’s capital, Riyadh. For the purpose of globalizing the findings, the same investigations were applied for two other cities of similar climates: Cairo, Egypt, and Arizona, the USA.

Design/methodology/approach
A test room was modelled for energy simulation, with a 20% window-to-wall ratio. A VB was assigned with alternatives of being located to the indoor, outdoor or in between double glass panes. High, medium and low reflectivity values were applied at each location at slat angle alternatives of 15°, 30°, 45°, 60°, 75° and 90°.

Findings
Results showed VB performance across slat angles, where up to 20.1% energy savings were achieved by mid-pane high reflectivity VBs in Riyadh, while the value exceeded 30% in case of being externally located. A similar performance pattern occurred in the other two cities of hot arid desert climates: Cairo and Arizona.

Research limitations/implications
The study is limited to VBs at a fixed position, with no upward movement for partial or full openness conditions. The effect of blind control and operation on performance, such as the amount and duration of openness/closure of the blind and changes in slat angle across time, in addition to VB automation, shall be investigated in a future study.

Practical implications
The better understanding of VB energy performance achieved would enhance a more rational selection of VBs, which would benefit the construction industry as it would assist designers, real estate developer companies, as well as end-users in the decision-making process and help to realize energy-efficient solutions in residential buildings. VB production entities would also benefit by manufacturing and promoting for energy-efficient products.

Originality/value
In this study, a matrix of combinations of three VB parameters was developed, and the effect of these combinations on the overall energy consumption of both artificial lighting and heating, ventilation and air conditioning (HVAC) systems was evaluated and compared to identify the combinations of higher efficiency. The literature showed that these three parameters were hardly investigated in a combined form and hardly assessed by considering the overall energy consumed by both artificial lighting and HVAC.
Sustainable supplier selection in a construction company: a new MCDM method based on dominance-based rough set analysis
Authors: Singh Arpit , Kumar Vimal , Verma Pratima 
Published: May 2023

Purpose
This study aims to focus on sustainable supplier selection in a construction company considering a new multi-criteria decision-making (MCDM) method based on dominance-based rough set analysis. The inclusion of sustainability concept in industrial supply chains has started gaining momentum due to increased environmental protection awareness and social obligations. The selection of sustainable suppliers marks the first step toward accomplishing this objective. The problem of selecting the right suppliers fulfilling the sustainable requirements is a major MCDM problem since various conflicting factors are underplay in the selection process. The decision-makers are often confronted with inconsistent situations forcing them to make imprecise and vague decisions.

Design/methodology/approach
This paper presents a new method based on dominance-based rough sets for the selection of right suppliers based on sustainable performance criteria relying on the triple bottom line approach. The method applied has its distinct advantages by providing more transparency in dealing with the preference information provided by the decision-makers and is thus found to be more intuitive and appealing as a performance measurement tool.

Findings
The technique is easy to apply using “jrank” software package and devises results in the form of decision rules and ranking that further assist the decision-makers in making an informed decision that increases credibility in the decision-making process.

Originality/value
The novelty of this study of its kind is that uses the dominance-based rough set approach for a sustainable supplier selection process.
Efficient keyword search on graph data for finding diverse and relevant answers
Authors: Park Chang-Sup 
Published: May 2023

Purpose
This paper studies a keyword search over graph-structured data used in various fields such as semantic web, linked open data and social networks. This study aims to propose an efficient keyword search algorithm on graph data to find top-k answers that are most relevant to the query and have diverse content nodes for the input keywords.

Design/methodology/approach
Based on an aggregative measure of diversity of an answer set, this study proposes an approach to searching the top-k diverse answers to a query on graph data, which finds a set of most relevant answer trees whose average dissimilarity should be no lower than a given threshold. This study defines a diversity constraint that must be satisfied for a subset of answer trees to be included in the solution. Then, an enumeration algorithm and a heuristic search algorithm are proposed to find an optimal solution efficiently based on the diversity constraint and an A* heuristic. This study also provides strategies for improving the performance of the heuristic search method.

Findings
The results of experiments using a real data set demonstrate that the proposed search algorithm can find top-k diverse and relevant answers to a query on large-scale graph data efficiently and outperforms the previous methods.

Originality/value
This study proposes a new keyword search method for graph data that finds an optimal solution with diverse and relevant answers to the query. It can provide users with query results that satisfy their various information needs on large graph data.