bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appropriate cold stimulation changes energy distribution to improve stress resistance in broilers
Authors: Gong Rixin , Xing Lu , Yin Jingwen , Ding Yuqing , Liu Xiaotao , Bao Jun , Li Jianhong 
Published: June 2023

Abstract
Appropriate cold stimulation can improve stress resistance in broilers and alleviate the adverse impacts of a cold environment. To investigate the effects of intermittent mild cold stimulation (IMCS) on energy distribution in the livers of broilers, 96 healthy 1-d-old Ross-308 male broilers were randomly divided into the control group (CC) and the cold stimulation group (H5). The CC group was raised at a normal thermal temperature, i.e., 35 °C until 3 d, after which the temperature was dropped gradually by 0.5 °C/d until 20 °C at 33 d. This temperature was maintained until 49 d. The H5 group was raised at the same temperature as the CC group until 14 d (35 to 29.5 °C) and at 3 °C below the temperature of the CC group starting at 0930 hours for 5 h every other day from 15 to 35 d (26 to 17°C). The temperature was returned to 20 °C at 36 d and maintained until 49 d. At 50 d, all broilers were subjected to acute cold stress (ACS) at 10 °C for 6 and 12 h. We found that IMCS had positive effects on production performance. Using transcriptome sequencing of the broiler livers, 327 differentially expressed genes (DEG) were identified, and highly enriched in fatty acid biosynthesis, fatty acid degradation, and the pyruvate metabolism pathway. When compared to the CC group, the mRNA levels of ACAA1, ACAT2, ACSL1, CPT1A, LDHB, and PCK1 in the H5 group were increased at 22 d (P &lt; 0.05). The LDHB mRNA level was upregulated in the H5 group at 29 d compared to the CC group (P &lt; 0.05). After 21 d of IMCS (at 36 d), the mRNA expression levels of ACAT2 and PCK1 were found to be significantly increased in the H5 group compared to the CC group (P &lt; 0.05). Seven days after the IMCS had ended (at 43 d), the mRNA levels of ACAA1, ACAT2, and LDHB in the H5 group were higher than in the CC group (P &lt; 0.05). The mRNA levels of heat shock protein (HSP) 70, HSP90, and HSP110 in the H5 group were higher than in the CC group after 6 h of ACS (P &lt; 0.05). The protein levels of HSP70 and HSP90 in the H5 group were downregulated after 12 h of ACS, compared to the CC group (P &lt; 0.05). These results indicated that IMCS at 3 °C lower than the normal temperature could improve energy metabolism and stress resistance in the livers of broilers, alleviate the damage of short-term ACS on broilers, help broilers adapt to the low temperature, and maintain stable of energy metabolism in the body.
Identification and functional characterization of developmental-stage-dependent piRNAs in Tibetan sheep testes
Authors: Li Taotao , Wang Huihui , Ma Keyan , Wu Yi , Qi Xingcai , Liu Zilong , Li Qiao , Zhang Yong , Ma Youji 
Published: June 2023

Abstract
The core function of the testes is to produce sperms, which is the prerequisite for maintaining male fertility. PIWI-interacting RNAs (piRNAs) are a class of non-coding small RNAs that are mainly enriched in the reproductive organ and play a key role in germ cell development and spermatogenesis. However, the expression and function of piRNAs in the testes of Tibetan sheep, a domestic animal endemic to the Tibetan Plateau, remain unknown. In this study, we evaluated the sequence structure, expression profile, and potential function of piRNAs in testicular tissues from Tibetan sheep at different developmental stages (3 months, 1 year, and 3 years of age, respectively) by small RNA sequencing. Of the identified piRNAs, the sequence lengths of 24–26 nt and 29 nt dominate. Most piRNA sequences begin with uracil and have a distinct ping-pong structure which mainly distributes in exons, repeat regions, introns, and other unannotated regions of the genome. The piRNAs in the repeat region are primarily derived from the retrotransposons: long terminal repeats, long interspersed nuclear elements, and short interspersed elements. These piRNAs constitute 2,568 piRNA clusters, which mainly distribute on chromosomes 1, 2, 3, 5, 11, 13, 14, and 24, and of these clusters, a total of 529 piRNA clusters were differentially expressed in at least two age groups. Most of the piRNAs were expressed in a low abundance in the testes of developing Tibetan sheep. A total of 41,552 and 2,529 differential piRNAs were identified in testes from 3 months vs. 1 year, and 1 year vs. 3 years, respectively, presenting significantly increased abundance for most piRNAs in 1 year and 3 years compared with 3 months. The functional evaluation of the target genes showed that the differential piRNAs are mainly involved in regulating gene expression, transcription, protein modification, and cell development during spermatogenesis and testicular development. In conclusion, this study focused on the sequence structure and expression characteristics of piRNAs in the testis of Tibetan sheep and provided new insights into the functional mechanism of piRNAs in testicular development and spermatogenesis of sheep.
Cell adhesion function was altered during the seasonal regression of the seminiferous epithelium in the mink species Neovison vison
Authors: Zhang Yufei , Zhu Yanzhu , Cao Xiaodong , Zhang Guanhua , Liu Shuying 
Published: June 2023

Abstract
Minks are seasonal breeders whose seminiferous epithelium undergoes regression through massive germ cell death, leaving only Sertoli cells and spermatogonial cells in the tubules. However, the molecular mechanisms that control this biological process remain largely unknown. This study describes a transcriptomic analysis of mink testes at various reproductive stages (active, regressing, and inactive). A comparison of seminiferous epithelium at different stages of reproduction shows that cell adhesion is altered during regression. In addition, genes and proteins involved in forming the blood–testis barrier (BTB) were examined in sexually active and inactive minks. The seminiferous epithelium in the testes of sexually inactive minks expressed occludin, but this expression was not discernibly observed in the testes of sexually active minks. There was no discernible expression of CX43 in the seminiferous epithelium in the testes of sexually inactive minks, but CX43 was expressed in the testes of sexually active minks. During the regression process, we observed a remarkable increase in the expression levels of Claudin-11, which is associated with Sertoli-germ cell junctions. In conclusion, these findings suggest a loss of Sertoli-germ cell adhesion, which may regulate postmeiotic cell shedding during testicular regression in mink.
Remarks on uniform interpolation property
Authors: Alizadeh Majid 
Published: June 2023

Abstract
A logic $\mathcal{L}$ is said to satisfy the descending chain condition, DCC, if any descending chain of formulas in $\mathcal{L}$ with ordering induced by $\vdash _{\mathcal{L}};$ eventually stops. In this short note, we first establish a general theorem, which states that if a propositional logic $\mathcal{L}$ satisfies both DCC and has the Craig Interpolation Property, CIP, then it satisfies the Uniform Interpolation Property, UIP, as well. As a result, by using the Nishimura lattice, we give a new simply proof of uniform interpolation for $\textbf{IPL}_2$, the two-variable fragment of Intuitionistic Propositional Logic; and one-variable uniform interpolation for $\textbf{IPL}$. Also, we will see that the modal logics $\textbf{S}_4$ and $\textbf{K}_4$ do not satisfy atomic DCC.
Real-world treatment outcomes of patients with penile cancer in the Kyushu–Okinawa area of Japan in the pre-guideline era
Authors: Yamaguchi Takahiro , Goya Masato , Higashijima Katsuyoshi , Tobu Shohei , Sato Ryuta , Tatarano Shuichi , Mukai Shoichiro , Uemura Kei-ichiro , Tatsugami Katsunori , Tsubouchi Kazuna , Shida Yohei , Ishii Tatsu , Sakai Hideki , Matsuoka Hirofumi , Haga Nobuhiro , Eto Masatoshi , Igawa Tsukasa , Kamoto Toshiyuki , Enokida Hideki , Shin Toshitaka , Noguchi Mitsuru , Fujimoto Naohiro , Saito Seiichi , Kamba Tomomi 
Published: June 2023

Abstract

Objectives
To understand the real-world outcomes for patients with penile cancer in the Kyushu–Okinawa area before the introduction of practice guidelines in Japan.

Methods
We retrospectively collected medical information on patients with penile squamous cell carcinoma and penile intraepithelial neoplasia at 12 university hospitals and their affiliated hospitals in the Kyushu–Okinawa area from January 2009 to December 2020. Patients with unknown clinical stage were excluded. Patient background characteristics and survival, as well as pretreatment factors involved in survival, were investigated.

Results
A total of 196 patients were included. Patients with clinical stage 0, I, IIA, IIB, IIIA, IIIB and IV comprised 9.7, 26.0, 22.4, 2.6, 10.7, 14.3 and 14.3%, respectively. The median follow-up was 26 months, and the mean 5-year overall survival and cancer-specific survival rates were 74.3 and 79.8%, respectively. On univariate analysis, tumor diameter ≥ 30 mm, penile shaft tumor, Eastern Cooperative Oncology Group performance status ≥ 1, cT ≥ 3, cN ≥ 2 and cM1 were associated with significantly poorer cancer-specific survival. On multivariate analysis, pretreatment factors of cN ≥ 2 (hazard ratio, 32.5; 95% confidence interval, 5.08–208; P = 0.0002), Eastern Cooperative Oncology Group performance status ≥ 1 (4.42; 1.79–10.9; P = 0.0012) and cT ≥ 3 (3.34; 1.11–10.1; P = 0.0319) were identified as independent prognostic factors.

Conclusions
The study revealed basic data for future penile cancer treatment and research, including survival rates according to clinical stages, and identified cN ≥ 2, Eastern Cooperative Oncology Group performance status ≥ 1 and cT ≥ 3 at initial diagnosis as independent prognostic factors. Evidence for penile cancer in Japan is particularly scarce, and future large-scale prospective studies are warranted.
Breast density quantitative measures and breast cancer risk among screened Black women
Authors: Mahmoud Mattia A , Ehsan Sarah , Pantalone Lauren , Mankowski Walter , Conant Emily F , Kontos Despina , Chen Jinbo , McCarthy Anne Marie 
Published: June 2023

Abstract
Mammographic density is a strong predictor of breast cancer but only slightly increased the discriminatory ability of existing risk prediction models in previous studies with limited racial diversity. We assessed discrimination and calibration of models consisting of the Breast Cancer Risk Assessment Tool (BCRAT), Breast Imaging-Reporting and Data System density and quantitative density measures. Patients were followed up from the date of first screening mammogram until invasive breast cancer diagnosis or 5-year follow-up. Areas under the curve for White women stayed consistently around 0.59 for all models, whereas the area under the curve increased slightly from 0.60 to 0.62 when adding dense area and area percent density to the BCRAT model for Black women. All women saw underprediction in all models, with Black women having less underprediction. Adding quantitative density to the BCRAT did not statistically significantly improve prediction for White or Black women. Future studies should evaluate whether volumetric breast density improves risk prediction.
Bacillus Calmette–Guérin osteomyelitis of the fifth metatarsal bone in a Japanese infant: a case report
Authors: Morimoto Shota , Iseki Tomoya , Tachibana Toshiya , Futani Hiroyuki 
Published: June 2023

AbstractBacillus Calmette–Guérin osteomyelitis is a rare complication following Bacillus Calmette–Guérin vaccination. Here, we describe a rare case of Bacillus Calmette–Guérin osteomyelitis of the fifth metatarsal in a 21-month-old Japanese infant. A 21-month-old Japanese female infant presented with a swollen mass on the dorsolateral aspect of the left foot. Based on physical examination, radiological and histopathologic findings and laboratory results, a diagnosis of Bacillus Calmette–Guérin osteomyelitis of the fifth metatarsal bone was made, and an oral anti-tuberculosis treatment was initiated. However, the mass recurred 10 months after the start of the anti-tuberculosis treatment, so additional surgical debridement was performed. Six months after surgery, clinical findings and plain radiograph images revealed complete improvement of the affected area, and anti-tuberculosis treatment was stopped. Bacillus Calmette–Guérin osteomyelitis of the fifth metatarsal in a 21-month-old Japanese infant was successfully treated with oral anti-tuberculosis therapy and surgical debridement.
Impact of female stress urinary incontinence and related treatments on the sexual function of male partners: a systematic review and meta-analysis
Authors: Tian Zhao , Wang Xiuqi , Fu Linru , Du Zhe , Sun Zhijing 
Published: June 2023

Abstract

Background
No conclusions have been reached on whether female stress urinary incontinence (SUI) and related treatments affect male partners’ sexual function.

Aim
To assess the effects of female SUI and related treatments on male partners’ sexual function.

Methods
A comprehensive search of the PubMed, Embase, Web of Science, Cochrane, and Scopus databases was performed up to September 6, 2022. Studies were included that investigated the effect of female SUI and related treatments on male partners’ sexual function.

Outcome
Male partners’ sexual function.

Results
Of the 2294 citations identified, 18 studies with 1350 participants were included. Two studies assessed the effect of female SUI without treatment on male partners’ sexual function, finding that partners had more erectile dysfunction, more sexual dissatisfaction, and less sexual frequency than partners of women without urinary incontinence. Seven studies directly assessed the effect of female SUI treatments on male partners’ sexual function by surveying the male partners. Among these, 4 assessed transobturator suburethral tape (TOT) surgery; 1 assessed TOT and tension-free vaginal tape obturator surgery; and the remaining 2 assessed pulsed magnetic stimulation and laser treatment. Among the 4 TOT studies, 3 used the International Index of Erectile Function (IIEF). TOT surgery significantly improved the total IIEF score (mean difference [MD] = 9.74, P &lt; .00001), along with erectile function (MD = 1.49, P &lt; .00001), orgasmic function (MD = 0.35, P = .001), sexual desire (MD = 2.08, P &lt; .00001), intercourse satisfaction (MD = 2.36, P &lt; .00001), and overall satisfaction (MD = 3.46, P &lt; .00001). However, the improvements in IIEF items may be of unclear clinical significance, as 4 points in the erectile function domain of the IIEF are typically defined as the minimal clinically important difference. In addition, 9 studies indirectly assessed the effect of female SUI surgery on male partners’ sexual function by surveying patients with the Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire. The results demonstrated no significant differences in erectile function (MD = 0.08, P = .40) or premature ejaculation (MD = 0.07, P = .54).

Clinical Implications
The effects of female SUI and related treatments on male partners’ sexual function were summarized for the first time, providing a reference for future clinical practice and scientific research.

Strengths and Limitations
A limited number of studies that used various scales met the standardized eligibility criteria.

Conclusion
Female SUI may affect male partners’ sexual function, and female patients’ anti-incontinence surgery does not appear to have a clinically significant improvement on the sexual function of their partners.
Methamphetamine-induced vaginal lubrication in rats
Authors: Mott Maggie N , Goeders Nicholas E 
Published: June 2023

Abstract

Background
Based on previous studies of vaginal lubrication as well as our own previously reported interview study of women who self-reported methamphetamine (meth)–induced vaginal lubrication, in the current study we sought to determine the potential dose-response relationship leading to meth-induced vaginal lubrication. We also developed an animal model to study the reported effects and examine potential mechanisms mediating this phenomenon.

Aim
We sought to characterize the effects of meth on vaginal lubrication in an animal model with the aim of providing a potential framework for new mechanisms that incorporate novel therapeutic agents for the treatment of vaginal dryness.

Methods
Vaginal lubrication was measured via insertion of a preweighed, cotton-tipped swab into the vaginal canal of anesthetized rats following treatment with various doses of intravenous (IV) meth, up to 0.96 mg/kg, and after additional pharmacological manipulations, including administration of a nitric oxide synthase inhibitor and an estrogen receptor antagonist. Plasma signaling molecules, including estradiol, progesterone, testosterone, nitric oxide, and vasoactive intestinal polypeptide, were measured immediately before and at 9 time points after IV meth administration. Blood was collected via a previously implanted chronic indwelling jugular catheter and analyzed by use of commercially available kits per the manufacturer’s instructions.

Outcomes
Outcomes for this study include the measurement of vaginal lubrication in anesthetized rats following various pharmacological manipulations and plasma levels of various signaling molecules.

Results
Meth dose-dependently increased vaginal lubrication in anesthetized female rats. Meth significantly increased plasma levels compared to baseline of estradiol (2 and 15 minutes after meth infusion) as well as progesterone, testosterone, and nitric oxide (10 minutes after meth infusion). Also, vasoactive intestinal polypeptide decreased significantly compared to baseline for 45 minutes following meth infusion. Our data further suggest that nitric oxide, but not estradiol, is critical in the production of vaginal secretions in response to meth.

Clinical Implications
This study has far-reaching implications for women who are suffering from vaginal dryness and for whom estrogen therapy is unsuccessful, as the investigation has demonstrated that meth presents a novel mechanism for producing vaginal lubrication that can be targeted pharmacologically.

Strengths and Limitations
This study is, to our knowledge, the first performed to measure the physiological sexual effects of meth in an animal model. Animals were anesthetized when they were administered meth. In an ideal situation, animals would be self-administering the drug to recapitulate better the contingent nature of drug taking; however, this method was not feasible for the study reported here.

Conclusion
Methamphetamine increases vaginal lubrication in female rats through a nitric oxide–dependent mechanism.
Effect of folic acid on the sexual function of postmenopausal women: a triple-blind randomized controlled trial
Authors: Asadi Mahnaz , Riazi Hedyeh , Abbasinazari Mohammad , Majd Hamid Alavi , Montazeri Ali 
Published: June 2023

Abstract

Background
There are reports of sexual dysfunction in postmenopausal women, and several treatment recommendations are available.

Aim
To investigate the effect of folic acid on postmenopausal women’s sexual function.

Methods
This triple-blind randomized controlled trial was conducted in Tehran, Iran, in 2020. A sample of 100 postmenopausal women was recruited from comprehensive health centers affiliated with the Shahid Beheshti University of Medical Sciences. Eligible women were randomly assigned to receive folic acid (5 mg) or placebo on an empty stomach every day for 8 weeks. Women were assessed at 3 time points: baseline and 4 and 8 weeks after the intervention.

Outcome
Sexual function was the main outcome, as measured by the Female Sexual Function Index.

Results
The mean ± SD age of participants in the folic acid and placebo groups was 53.2 ± 3.84 and 54.4 ± 4.05 years, respectively (P = .609). The results obtained from mixed effects analysis of variance revealed a statistically significant difference between baseline and posttreatment scores and the interaction between time and group for desire, orgasm, satisfaction, arousal, pain, and total sexual function score, with the folic acid group improving more than control group. Lubrication was the only domain that showed no significant difference for the interaction between time and group.

Clinical Implications
Folic acid may beneficially affect sexual function in postmenopausal women.

Strengths and Limitations
Strengths include the novelty of the subject, the triple-blind design, the block randomization, the administration of a standard scale for sexual function (Female Sexual Function Index), and the affordability and availability of folic acid. This study was conducted with a small sample size and short follow-up time; therefore, interpretation of the results requires great caution.

Conclusion
The findings suggest that folic acid possibly improves sexual function in postmenopausal women. Larger studies are needed to confirm the findings.

Trial Registration
IRCT20150128020854N8; August 2, 2020. Iranian Registry of Clinical Trials; https://en.irct.ir/user/trial/48920/view.
Travel-related respiratory symptoms and infections in travellers (2000–22): a systematic review and meta-analysis
Authors: Lovey Thibault , Hasler Robin , Gautret Philippe , Schlagenhauf Patricia 
Published: June 2023

Abstract

Background
Respiratory tract infections (RTIs) are common in travellers due to the year-round or seasonal presence of respiratory pathogen and exposure to crowded environments during the itinerary. No study has systematically examined the burden of RTI infections among travellers. The aim of this systematic review and meta-analysis is to evaluate the prevalence of RTIs and symptoms suggestive of RTIs among travellers according to risk groups and/or geographic region, and to describe the spectrum of RTIs.

Methods
The systematic review and meta-analysis was registered in PROSPERO (CRD42022311261). We searched Medline, Embase, Scopus, Cochrane Central, Web of Science, Science Direct and preprint servers MedRxiv, BioRxiv, SSRN and IEEE Xplore on 1 February 2022. Studies reporting RTIs or symptoms suggestive of RTIs in international travellers after 1 January 2000 were eligible. Data appraisal and extraction were performed by two authors, and proportional meta-analyses were used to obtain estimates of the prevalence of respiratory symptoms and RTIs in travellers and predefined risk groups.

Findings
A total of 429 articles on travellers’ illness were included. Included studies reported 86 841 symptoms suggestive of RTIs and 807 632 confirmed RTIs. Seventy-eight percent of reported respiratory symptoms and 60% of RTIs with available location data were acquired at mass gatherings events. Cough was the most common symptom suggestive of respiratory infections, and the upper respiratory tract was the most common site for RTIs in travellers. The prevalence of RTIs and respiratory symptoms suggestive of RTIs were 10% [8%; 14%] and 37% [27%; 48%], respectively, among travellers. Reporting of RTIs in travellers denoted by publication output was found to correlate with global waves of new respiratory infections.

Interpretation
This study demonstrates a high burden of RTIs among travellers and indicates that travellers’ RTIs reflect respiratory infection outbreaks. These findings have important implications for understanding and managing RTIs among travellers.