bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photopolymerizable Hydrogel for Enhanced Intramyocardial Vascular Progenitor Cell Delivery and Post‐Myocardial Infarction Healing
Authors: Hong Xuechong , Luo Allen Chilun , Doulamis Ilias , Oh Nicholas , Im Gwang‐Bum , Lin Chun‐Yen , del Nido Pedro J. , Lin Ruei‐Zeng , Melero‑Martin Juan M. 
Published: August 2023

AbstractCell transplantation success for myocardial infarction (MI) treatment is often hindered by low engraftment due to washout effects during myocardial contraction. A clinically viable biomaterial that enhances cell retention can optimize intramyocardial cell delivery. In this study, a therapeutic cell delivery method is developed for MI treatment utilizing a photocrosslinkable gelatin methacryloyl (GelMA) hydrogel. Human vascular progenitor cells, capable of forming functional vasculatures upon transplantation, are combined with an in situ photopolymerization approach and injected into the infarcted zones of mouse hearts. This strategy substantially improves acute cell retention and promotes long‐term post‐MI cardiac healing, including stabilized cardiac functions, preserved viable myocardium, and reduced cardiac fibrosis. Additionally, engrafted vascular cells polarize recruited bone marrow‐derived neutrophils toward a non‐inflammatory phenotype via transforming growth factor beta (TGFβ) signaling, fostering a pro‐regenerative microenvironment. Neutrophil depletion negates the therapeutic benefits generated by cell delivery in ischemic hearts, highlighting the essential role of non‐inflammatory, pro‐regenerative neutrophils in cardiac remodeling. In conclusion, this GelMA hydrogel‐based intramyocardial vascular cell delivery approach holds promise for enhancing the treatment of acute myocardial infarction.
Virus‐Based Pyroelectricity
Authors: Kim Han , Okada Kento , Chae Inseok , Lim Butaek , Ji Seungwook , Kwon Yoonji , Lee Seung‐Wuk 
Published: August 2023

AbstractThe first observation of heat‐induced electrical potential generation on a virus and its detection through pyroelectricity are presented. Specifically, the authors investigate the pyroelectric properties of the M13 phage, which possesses inherent dipole structures derived from the noncentrosymmetric arrangement of the major coat protein (pVIII) with an α‐helical conformation. Unidirectional polarization of the phage is achieved through genetic engineering of the tail protein (pIII) and template‐assisted self‐assembly techniques. By modifying the pVIII proteins with varying numbers of glutamate residues, the structure‐dependent tunable pyroelectric properties of the phage are explored. The most polarized phage exhibits a pyroelectric coefficient of 0.13 µC m−2 °C−1. Computational modeling and circular dichroism (CD) spectroscopy analysis confirm that the unfolding of α‐helices within the pVIII proteins leads to changes in phage polarization upon heating. Moreover, the phage is genetically modified to enable its pyroelectric function in diverse chemical environments. This phage‐based approach not only provides valuable insights into bio‐pyroelectricity but also opens up new opportunities for the detection of various viral particles. Furthermore, it holds great potential for the development of novel biomaterials for future applications in biosensors and bioelectric materials.
Cucurbit[8]uril Induced Molecular Folding Cascade Assembly for NIR Targeted Cell Imaging
Authors: Zhou Xiaolu , Bai Xue , Zhang Xuejian , Wu Jing , Liu Yu 
Published: August 2023

AbstractA macrocyclic confinement‐activated green phosphorescence and two‐photon near‐infrared (NIR) fluorescence supramolecular cascade assembly is constructed by multivalent interaction based on the alkyl‐bridged triphenylamine‐vinyl pyridine salts (BTPA), cucurbit[8]uril (CB[8]), β‐cyclodextrin‐grafted hyaluronic acid (HACD), and 4‐bromophenyl pyridine salt and CB[8] complex (PY/CB[8]). Taking advantage of CB[8]‐induced macrocyclic confinement and molecular folding, the fluorescence of BTPA is effectively redshifted by 30 nm from 720 to 750 nm. Subsequently, HACD, a cancer cell targeting agent, is co‐assembled with a BTPA/CB[8] supramolecular folder to form a nanoparticle, further enhancing NIR fluorescence from 750 nm up to 810 nm through cascade confinement. Upon introduction of the 2:1 complex PY/CB[8], multicomponent nanoparticles are fabricated, not only showing efficient NIR fluorescence with an increase in quantum yield from 7.21% to 22.92% but also endowing green phosphorescence emission, which is successfully applied for two‐photon cancer cell‐targeted imaging to shield background interference from fluorescent and phosphorescent resources.
Photoinduced Synthesis of Polycyclic γ‐Sultines by a Radical‐Polar Crossover Cyclization
Authors: Zhang Yongxin , Zhou Pan , Yang Xiaoxiao , Li Helian , Deng Zhenxi , Ren Mingtian , Shu Chao 
Published: August 2023

AbstractDescribed herein is a visible light‐mediated radical addition/SO2 insertion/anionic cyclization of benzo‐fused homoallylic tosylates, allowing access to a diverse array of otherwise challenging‐to‐access fluoromethylated polycyclic γ‐sultines with two adjacent tetrasubstituted carbon stereocenters in 49–95% yields, which can be further readily functionalized towards bulky two adjacent tetrasubstituted carbon‐containing benzo‐fused γ‐sultone and mercaptoalkanol. Sodium fluoroalkanesulfinates were utilized as bifunctionalization reagents for both fluoromethyl groups and SO2. The cyclization proceeds through a stepwise photoinduced radical addition followed by an intramolecular 5‐exo‐tet anionic substitution cyclization after SO2 insertion.
DFT Study of High‐Curie‐Temperature Ferromagnetism in α‐borophene Nanoribbons for Spintronic Applications
Authors: Vatankhahan Adeleh , Movlarooy Tayebeh 
Published: August 2023

AbstractThe aim is to tune the stability, as well as electronic and magnetic characteristics of zigzag α‐borophene nanoribbons (ZαBNRs) based on density functional theory. For this reason, the bare ZαBNR and its passivated edges by a single nitrogen atom (N‐ZαBNR) and also Mn‐ and Cr‐doped N‐ZαBNR is investigated. The results indicate that the nanoribbon becomes more stable by passivating the edges of the structure. Furthermore, the calculated electronic structures show that the Cr atom can remove the band gap of N‐ZαBNRs and turn it into metal. Besides, it can create magnetic properties in this structure. The Cr‐ and Mn‐doped N‐ZαBNR turn it into magnetic metal and diluted magnetic semiconductors, respectively. The results reveal a high Curie temperature of more than 700K for Cr‐ and Mn‐doped N‐ZαBNRs, indicating maybe good room‐temperature ferromagnetic material for spintronic applications in the future.
Impact of recurrent acute rhinosinusitis on quality of life
Authors: Al‐Asadi Zayd , Cui Ruifeng , Lombardo Dominic , Dewey John , Ramadan Hassan H. , Makary Chadi A. 
Published: August 2023

Key Points
Quality of life (QoL) in patients with recurrent acute rhinosinusitis (RARS) is understudied.
QoL for RARS patients is similar to chronic rhinosinusitis patients, although objective disease severity is lower.
QoL of RARS patients is similarly affected during active and inactive infection.
Mapping sleep's oscillatory events as a biomarker of Alzheimer's disease
Authors: Pulver Rachelle L. , Kronberg Eugene , Medenblik Lindsey M. , Kheyfets Vitaly O. , Ramos Alberto R. , Holtzman David M. , Morris John C. , Toedebusch Cristina D. , Sillau Stefan H , Bettcher Brianne M. , Lucey Brendan P. , McConnell Brice V. 
Published: August 2023

AbstractINTRODUCTIONMemory‐associated neural circuits produce oscillatory events including theta bursts (TBs), sleep spindles (SPs), and slow waves (SWs) in sleep electroencephalography (EEG). Changes in the “coupling” of these events may indicate early Alzheimer's disease (AD) pathogenesis.METHODSWe analyzed 205 aging adults using single‐channel sleep EEG, cerebrospinal fluid (CSF) AD biomarkers, and Clinical Dementia Rating® (CDR®) scale. We mapped SW‐TB and SW‐SP neural circuit coupling precision to amyloid positivity, cognitive impairment, and CSF AD biomarkers.RESULTSCognitive impairment correlated with lower TB spectral power in SW‐TB coupling. Cognitively unimpaired, amyloid positive individuals demonstrated lower precision in SW‐TB and SW‐SP coupling compared to amyloid negative individuals. Significant biomarker correlations were found in oscillatory event coupling with CSF Aβ42/Aβ40, phosphorylated‐ tau181, and total‐tau.DISCUSSIONSleep‐dependent memory processing integrity in neural circuits can be measured for both SW‐TB and SW‐SP coupling. This breakdown associates with amyloid positivity, increased AD pathology, and cognitive impairment.Highlights
At‐home sleep EEG is a potential biomarker of neural circuits linked to memory.
Circuit precision is associated with amyloid positivity in asymptomatic aging adults.
Levels of CSF amyloid and tau also correlate with circuit precision in sleep EEG.
Theta burst EEG power is decreased in very early mild cognitive impairment.
This technique may enable inexpensive wearable EEGs for monitoring brain health.
Capture of Singlet Oxygen Modulates Host‐Guest Behavior of Coordination Cages
Authors: Jahović Ilma , Yang Yuchong , Ronson Tanya K. , Nitschke Jonathan R. 
Published: August 2023

AbstractThe anthracene panels of two tetrahedral MII4L6 cages, where MII=CoII or FeII, were found to react with photogenerated singlet oxygen (1O2) in a hetero‐Diels–Alder reaction. ESI‐MS analysis showed the cobalt(II) cages to undergo complete transformation of all anthracene panels into endoperoxides, whereas the iron(II) congeners underwent incomplete conversion. The reaction was found to be partially reversible in the case of the 1‐FeII cage. The dioxygen‐cage cycloadducts were found to bind a set of guest molecules more weakly than the parent cages, with affinity dropping by more than two orders of magnitude in some cases. The light‐driven cycloaddition reaction between cage and 1O2 thus served as a stimulus for guest release and reuptake.
The S1′–S3′ Pocket of the SARS‐CoV‐2 Main Protease Is Critical for Substrate Selectivity and Can Be Targeted with Covalent Inhibitors
Authors: Liu Ming , Li Jihui , Liu Wenqi , Yang Ying , Zhang Manman , Ye Yuxin , Zhu Wenning , Zhou Cuiyan , Zhai Hongbin , Xu Zhengshuang , Zhang Guoliang , Huang Hao 
Published: August 2023

AbstractThe main protease (Mpro) of SARS‐CoV‐2 is a well‐characterized target for antiviral drug discovery. To date, most antiviral drug discovery efforts have focused on the S4–S1′ pocket of Mpro; however, it is still unclear whether the S1′–S3′ pocket per se can serve as a new site for drug discovery. In this study, the S1′–S3′ pocket of Mpro was found to differentially recognize viral peptidyl substrates. For instance, S3′ in Mpro strongly favors Phe or Trp, and S1′ favors Ala. The peptidyl inhibitor D‐4–77, which possesses an α‐bromoacetamide warhead, was discovered to be a promising inhibitor of Mpro, with an IC50 of 0.95 μM and an antiviral EC50 of 0.49 μM. The Mpro/inhibitor co‐crystal structure confirmed the binding mode of the inhibitor to the S1′–S3′ pocket and revealed a covalent mechanism. In addition, D‐4–77 functions as an immune protectant and suppresses SARS‐CoV‐2 Mpro‐induced antagonism of the host NF‐κB innate immune response. These findings indicate that the S1′–S3′ pocket of SARS‐CoV‐2 Mpro is druggable, and that inhibiting SARS‐CoV‐2 Mpro can simultaneously protect human innate immunity and inhibit virion assembly.
Giant Mid‐Infrared Second‐Harmonic Generation Response in a Densely‐Stacked Van Der Waals Transition‐Metal Oxychloride
Authors: Jin Congcong , Jiang Xingxing , Wu Chao , Duanmu Kaining , Lin Zheshuai , Huang Zhipeng , Humphrey Mark G. , Zhang Chi 
Published: August 2023

AbstractSecond‐harmonic generation (SHG) is a fundamental optical property of nonlinear optical (NLO) crystals. Thus far, it has proved difficult to engineer large SHG responses, particularly in the mid‐infrared region, owing to the difficulty in simultaneously controlling the arrangement and density of functional NLO‐active units. Herein, a new assembly strategy employing functional modules only, and aimed at maximizing the density and optimizing the spatial arrangement of highly efficient functional modules, has been applied to the preparation of NLO crystals, affording the van der Waals crystal MoO2Cl2. This exhibits the strongest powder SHG response (2.1×KTiOPO4 (KTP) @ 2100 nm) for a transition‐metal oxyhalide, a wide optical transparency window, and a sufficient birefringence. MoO2Cl2 is the first SHG‐active transition‐metal oxyhalide effective in the infrared region. Theoretical studies and crystal structure analysis suggest that the densely packed, optimally‐aligned [MoO4Cl2] modules within the two‐dimensional van der Waals layers are responsible for the giant SHG response.
Synthesis of C60/[10]CPP‐Catenanes by Regioselective, Nanocapsule‐Templated Bingel Bis‐Addition
Authors: Steudel Fabian M. , Ubasart Ernest , Leanza Luigi , Pujals Míriam , Parella Teodor , Pavan Giovanni M. , Ribas Xavi , von Delius Max 
Published: August 2023

AbstractThe addition of two unsymmetric malonate esters to the Buckminster fullerene C60 can lead to 22 spectroscopically distinguishable isomeric products and therefore represents a formidable synthesis challenge. In this work, we achieve 87 % selectivity for the formation of a single (in,out‐trans‐3) isomer by combining three approaches: (i) we use a starting material, in which the two malonates are covalently connected (tether approach); (ii) we form the strong supramolecular complex of C60 with the shape‐persistent [10]CPP macrocycle (template approach) and (iii) we embed this complex further within a self‐assembled nanocapsule (shadow mask approach). Variation of the spacer chain shed light on the limitations of the approach and the ring dynamics in the unusual [2]catenanes were studied in silico with atomistic resolution. This work significantly widens the scope of mechanically interlocked architectures comprising cycloparaphenylenes (CPP).
Application of Metal‐Organic Frameworks in Imaging‐Guided Therapy
Authors: Zhu Man , Ke Ming , Zhao Wenjing , Wu Qingqing , Li Shaoguang , Li Hui , Xia Fan 
Published: August 2023

AbstractMetal‐organic frameworks (MOFs) are formed by the self‐assembly of metal centres/clusters and organic ligands. A diverse range of organic ligands can be employed, including nucleobases, amino acids, peptides, proteins, and saccharides et al. Due to the characteristics of large surface area, high porosity, easy surface functionalization and adjustable internal pore size, et al. MOFs have been used as a significant emerging class of biological imaging agents and have attracted great research interest in optical imaging (OI), photoacoustic imaging (PAI), magnetic resonance imaging (MRI), computer tomography (CT) and positron emission tomography (PET) in recent years. This review provides a comprehensive overview of the diverse range of organic ligands utilized in Metal‐organic frameworks, while also delving into the recent advancements, opportunities, and challenges encountered in the realm of bioimaging in recent years.
Effect of different processing practices on physicochemical parameters, phenolic compounds, and antioxidant properties of chia seed oils
Authors: Chen Chuang , Wang Yongjin , Cui Ru , Zhu Ling , Han Jing , Sun Jiping , Xu Qi , Karrar Emad , Zhang Hui , Jin Qingzhe , Wu Gangcheng , Wang Xingguo 
Published: August 2023

AbstractAlpha‐linolenic acid and antioxidant substances (such as tocopherols and polyphenols) are abundant in chia seed oil, which may prevent certain chronic diseases. This study compared the physicochemical parameters, chemical composition and antioxidant properties of black and white chia seed oil extracted by solvent extraction, pressing and supercritical CO2 extraction. Chia seed oil contained higher levels of polyunsaturated fatty acids, including linoleic acid (18.68%–20.15%) and α‐linolenic acid (60.47%–63.68%). Black and white chia seeds and processing methods had significant effects on minor components (tocopherols, total phenols, phytosterols and squalene), oxidative stability and free radical scavenging ability. Compared with other methods, the contents of tocopherols (512.72–603.04 mg/kg), total phenols (35.21–57.75 mgGAE/kg) and phytosterols (5536.69–5956.65 mg/kg) were higher in the pressing method. In addition, 17 individual polyphenols were identified and quantified, among which genistein and gallic acid had higher contents, which were significantly affected by varieties and processing methods. In general, the pressing process may be the best processing method to improve minor components and antioxidant capacity. This study can provide a valuable reference for producing functional foods supplemented with α‐linolenic acid.
Photobase generators with AIE property used for visual observations of thiol–epoxy near‐UV/visible photopolymerization
Authors: Xie Gang , Huang Yugang , Hu Huawen , Yang Jianwen 
Published: August 2023

AbstractPhotopolymerization of thiol–epoxy resins initiated by photobase generators (PBGs) is a very good way to prepare specific polymeric products. However, the lack of near‐UV or visible light emitting diode (LED) light‐sensitive PBGs that can release strong bases to effectively catalyze this reaction and synchronously monitor the polymerization progress still is a problem. In this work, a series of such PBGs are successfully synthesized by modifying common aggregation‐induced emission (AIE) molecules, including triphenylamine and tetraphenylethylene and then neutralizing them with strong bases like 1,8‐diazabicyclo[5.4.0]undec‐7‐ene, 1,5‐diazabicyclo[4.3.0]non‐5‐ene, and 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene (TBD). The obtained PBGs show very good light absorption ability from 365 to 405 nm. They can generate free strong bases under near‐UV/visible LED light radiations as a result of photodecarboxylation reaction to catalyze the rapid thiol–epoxy photopolymerization. The TBD‐based PBGs have the best final monomer conversion above 60% due to their strongest alkalinity. Increasing viscosity during the polymerization process can trigger an AIE phenomenon of the PBGs. A linear correlation equation of the AIE intensity with the monomer conversion can be found, which is helpful for quantitatively monitoring the photopolymerization progress. More importantly, qualitative observations of the brightness changes of the resulting AIE lights are very useful to visualize the photopolymerization progress, which may find some applied potential in industry.
New insights into immune genes and other expanded gene families of the house fly, Musca domestica, from an improved whole genome sequence
Authors: Meisel Richard P. , Freeman Jamie C. , Asgari Danial , Llaca Victor , Fengler Kevin A. , Mann David , Rastogi Achal , Loso Mike , Geng Chaoxian , Scott Jeffrey G. 
Published: August 2023

AbstractThe house fly, Musca domestica, is a pest of livestock, transmits pathogens of human diseases, and is a model organism in multiple biological research areas. The first house fly genome assembly was published in 2014 and has been of tremendous use to the community of house fly biologists, but that genome is discontiguous and incomplete by contemporary standards. To improve the house fly reference genome, we sequenced, assembled, and annotated the house fly genome using improved techniques and technologies that were not available at the time of the original genome sequencing project. The new genome assembly is substantially more contiguous and complete than the previous genome. The new genome assembly has a scaffold N50 of 12.46 Mb, which is a 50‐fold improvement over the previous assembly. In addition, the new genome assembly is within 1% of the estimated genome size based on flow cytometry, whereas the previous assembly was missing nearly one‐third of the predicted genome sequence. The improved genome assembly has much more contiguous scaffolds containing large gene families. To provide an example of the benefit of the new genome, we used it to investigate tandemly arrayed immune gene families. The new contiguous assembly of these loci provides a clearer picture of the regulation of the expression of immune genes, and it leads to new insights into the selection pressures that shape their evolution.
An in silico analysis on the photoproteins Mnemiopsin 1 and Mnemiopsin 2 to explain the experimental results
Authors: Asadi Sofilar Ashraf , Shirdel Akram , Jafarian Vahab , Khalifeh Khosrow 
Published: August 2023

AbstractMnemiopsin 1 (Mn1) and Mnemiopsin 2 (Mn2) are photoproteins found in Mnemiopsis leidyi. We have tried to answer the question of whether the structural features of photoproteins can explain the observed activity data. According to the activity measurements data, they have the same characteristic wavelength. However, the initial intensity of Mn2 is significantly higher than that of Mn1, and decay time of Mn1 (0.92 s−1) is lower than that of Mn2 (1.46 s−1). The phylogenetic analysis demonstrates that, compared with Obelin and Aequorin from Obelia longissima and Aequorea victoria, respectively, a gene modification event may have caused the expansion of the N‐terminal side of all photoproteins from M. leidyi. An in silico study has shown that the stability of the photoprotein–substrate complex of Mn2 is higher than that of Mn1, indicating a higher affinity of the substrate for Mn2 compared with Mn1. It was revealed that the active EF‐hand loops 1 and III in Mn2 is locally more rigid compared with those in Mn1. We concluded that different stability of the photoprotein complexes leads to different initial intensity. While different patterns of the local dynamics of loops I and III may influence the decay rate.
Comprehensive Phytochemical Content by LC/MS/MS and Anticholinergic, Antiglaucoma, Antiepilepsy, and Antioxidant Activity of Apilarnil (Drone Larvae)
Authors: Inci Hakan , Izol Ebubekir , Yilmaz Mustafa Abdullah , Ilkaya Mehmet , Bingöl Zeynebe , Gülçin Ilhami 
Published: August 2023

AbstractApilarnil is 3–7 days old drone larvae. It is an organic bee product known to be rich in protein. In this study, the biological activities of Apilarnil were determined by its antioxidant and enzyme inhibition effects. Antioxidant activities were determined by Fe3+, Cu2+, Fe3+‐TPTZ ((2,4,6‐tris(2‐pyridyl)‐s‐triazine), reducing ability and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH⋅) scavenging assays. Also, its enzyme inhibition effects were tested against carbonic anhydrase I and II isoenzymes (hCA I, hCA II), acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Antioxidant activity of Apilarnil was generally lower than the standard molecules in the applied methods. In DPPH⋅ radical scavenging assay, Apilarnil exhibited higher radical scavenging than some standards. Enzyme inhibition results towards hCA I (IC50: 14.2 μg/mL), hCA II: (IC50: 11.5 μg/mL), AChE (IC50: 22.1 μg/mL), BChE (IC50: 16.1 μg/mL) were calculated. In addition, the quantity of 53 different phytochemical compounds of Apilarnil was determined by a validated method by LC/MS/MS. Compounds with the highest concentrations (mg analyte/g dry extract) were determined as quinic acid (1091.045), fumaric acid (48.714), aconitic acid (47.218), kaempferol (39.946), and quercetin (27.508). As a result, it was determined that Apilarnil had effective antioxidant profile when compared to standard antioxidants.