bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Online Orton-Gillingham Lessons to Teach Reading Comprehension and Word Recognition to Preschool Children at Risk for Dyslexia
Authors: Ali Mohammed Hussien Bothina 
Published: April 2023

Purpose. The purpose of this study was to gain insight into the potential effects of online Orton-Gillingham-based instruction on improving some reading skills among children identified at risk for dyslexia.
Methods. This study employed a quantitative approach using a quasi-experimental, pre-test and post-test and follow up design to investigate the effects of Orton-Gillingham-based instruction on reading comprehension and word recognition among preschool children at risk for dyslexia. Sixty children were chosen. Children received 3 training sessions a week, lasting between 40 and 45 min: (10) minutes for auditory training, (15) minutes for visual training, (10) for motor training and (10) minutes for pairing training.
Results. The results indicated that using Orton-Gillingham method increased reading comprehension and word recognition among Arabic-speaking children at risk for dyslexia.
Conclusion. Online Orton-Gillingham-based instruction was effective in improving some reading skills among children identified at risk for dyslexia. It is recommended that this method to be incorporated in into the curriculum in order to best-teach children at risk for dyslexia and other LDs. Teachers, particularly who are concerned with teaching reading to those type of children should be equipped with adequate knowledge and information about Orton-Gillingham-based instruction.
Predictors of Nonadherence to Planned Coronary Angiography: A Retrospective Analysis
Authors: Petsuwan Sutida , Maneewat Khomapak , Sae-Sia Wipa , Chittithavorn Voravit 
Published: April 2023

Objective: This study aimed to determine the predictive power of patient-related factors, socio-economic factors, condition-related factors, treatment-related factors, and health care system-related factors for nonadherence to planned coronary angiography (CAG). Material and Methods: A retrospective analysis was conducted on electronic medical records (EMR) of 665 patients appointed for elective CAG at Naradhiwas Rajanagarindra Heart Center from January 2018 to December 2019. One hundred and thirty-three patients with nonadherence to planned CAG were assigned to the study group; the control group consisted of 532 patients with adherence to planned CAG. Results: The retrospective data analysis revealed that divorced or widowed status (OR=3.07; 95% CI 1.54, 6.12), cerebrovascular disease comorbidity (OR=4.37; 95% CI 1.74, 10.96), prescribed diuretics (OR=2.24; 95% CI 1.26, 3.97), CAG wait time three months or longer (OR=3.34; 95% CI 1.46, 7.64) and history of parental cardiovascular disease or death from heart disease (OR=0.12; 95% CI 0.01, 0.95) were co-predictors of nonadherence to planned CAG. Socioeconomic- related factors had no predictive power for planned CAG nonadherence. Conclusion: The findings of this study may contribute to the improvement of nursing service by screening groups at high risk of nonadherence and developing appropriate interventions aimed at increasing adherence to planned CAG as well the rate of positive health outcomes.
Connected domination in a signed graph and its complement
Authors: Jeyalakshmi P. , Karuppasamy K. 
Published: April 2023

A signed graph Σ = (G, σ) is a graph with a sign attached to each arc. A subset S of V (Σ) is called a dominating set of Σ if |N+ (v) ∩ S| > |N- (v) ∩ S| for all v ∈ V - S . A dominating set S ⊆ V is a connected dominating set of Σ if <S> is connected. The minimum cardinality of a connected dominating set of Σ denoted by γsc, is called the connected domination number of Σ . In this paper, we introduce the connected domination number in a signed graph Σ and study different bounds and characterization of the connected domination number in a signed graph Σ . Furthermore, we find the best possible upper and lower bounds for γ sc ( Σ ) + γ sc ( Σ α c ) where Σ is connected.
Possible Description of the J/Ψ Ξ (S = -2) Structure in Terms of a First-Order Pentaquark Mass Formula
Authors: Bevelacqua Joseph 
Published: April 2023

A recently proposed PΞΨss pentaquark structure is investigated using a first-order mass formula incorporating weakly interacting J/Ψ meson and Ξ baryon clusters. The results are compared to the theoretical predictions of Marsé-Valera , Magas , and Ramos.

The first-order-mass formula predicts the J/Ψ Ξ pentaquark has a mass of 4567 MeV/c2. This prediction lies between the values predicted by Marsé-Valera, Magas , and Ramos (i.e., 4493 MeV/c2 (PB interaction) and 4633 MeV/c2 (VB interaction)).

The first-order mass formula also predicts Jπ values of 1/2¯ and 3/2¯ for the proposed J/Ψ Ξ pentaquark. This is consistent with the predictions of the PB (VB) interaction assumption of Jπ = 1/2¯ (1/2¯ and 3/2¯).
Task-Supported EFL Instruction: Integrating Students, Teacher, and Textbook Roles in a Japanese Mainstream High School Class
Authors: Santos Alvin Ibe 
Published: April 2023

Today in Japan, the impetus to effect positive change on students’ use of English as a communication tool is reflected in the thrust of the Ministry of Education to promote meaningful in-class practice as highlighted in the revised high school foreign language curriculum. However, teachers face a dilemma of implementing meaningful classroom practice of English entirely without any focus on grammar or form. Fortunately, an approach which may offer a balanced opportunity to create meaningful practice while highlighting the target expression in use is Task-supported language teaching (TSLT). To better understand TSLT’s usefulness in an EFL setting, this classroom-based research explores the potential roles that students, the teacher, and the textbook play in a sociocultural approach-oriented EFL class employing TLST and how it complements a Japanese mainstream high school class. Utilizing mixed methods of sociocultural discourse analysis and self-reporting of Grade 11 (n=117) mainstream EFL learners, results indicate that: the students, talking in pairs using their L1, (1) understood difficult vocabulary, (2) managed assigned tasks, and (3) engaged in off-task dialogue; the teacher performed the roles of (1) classroom manager, (2) communicator of content, and (3) assessor and provider of feedback; and the textbook played (1) informative, (2) instructional, and (3) experiential roles in the classroom. It was also found that TLST complements a Japanese high school mainstream EFL class by (1) optimizing the limited time offered in class for students to tackle the TL; (2) aiding students in their standardized institutional exams; and (3) appealing to Japanese cultural sensibilities.
Filling Information Gap on Covid-19 Pandemic Using Proverbs and Figurative Language: Lilaphalapha Facebook Page Lesotho
Authors: Mokala Ntsoaki Teresa , Matee Lihotetso Gloria , Khetoa Soyiso Godsave 
Published: April 2023

The current study reports on ways in which Lilaphalapha page on Facebook used language to provide awareness on Corona virus pandemic. Corona Virus affected the whole universe in different ways. Like the rest of the world, to curb the spread of the virus, Lesotho declared a state of emergency and the government put different health protocols in place. To prevent the widespread of COVID-19, a wealth of knowledge was provided in Indo-European languages in many countries. Speakers of African languages such as Sesotho, a national language of Lesotho, had to be innovative and employ region specific methodologies to spread awareness about COVID-19 pandemic. In this qualitative research, data was collected from eight purposely selected episodes posted on Lilaphalapha page on Facebook in April 2020. The videos were analysed through inductive thematic analysis approach. The paper analysed how the use of proverbs and figurative language has helped in preserving Sesotho as a language and filled the gap in preventing language dearth particularly in the advent of COVID-19 pandemic. The findings indicated figurative language, humour and proverbs were used in spreading COVID-19 awareness to Basotho.
Improved understanding of biorisk for research involving microbial modification using annotated sequences of concern
Authors: Godbold Gene D. , Hewitt F. Curtis , Kappell Anthony D. , Scholz Matthew B. , Agar Stacy L. , Treangen Todd J. , Ternus Krista L. , Sandbrink Jonas B. , Koblentz Gregory D. 
Published: April 2023

Regulation of research on microbes that cause disease in humans has historically been focused on taxonomic lists of ‘bad bugs’. However, given our increased knowledge of these pathogens through inexpensive genome sequencing, 5 decades of research in microbial pathogenesis, and the burgeoning capacity of synthetic biologists, the limitations of this approach are apparent. With heightened scientific and public attention focused on biosafety and biosecurity, and an ongoing review by US authorities of dual-use research oversight, this article proposes the incorporation of sequences of concern (SoCs) into the biorisk management regime governing genetic engineering of pathogens. SoCs enable pathogenesis in all microbes infecting hosts that are ‘of concern’ to human civilization. Here we review the functions of SoCs (FunSoCs) and discuss how they might bring clarity to potentially problematic research outcomes involving infectious agents. We believe that annotation of SoCs with FunSoCs has the potential to improve the likelihood that dual use research of concern is recognized by both scientists and regulators before it occurs.
Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer
Authors: Wang Feng , Yu Xiaolei , Yu Zhuo , Cui Yi , Xu Ling , Huo Shuhao , Ding Zhongyang , Zhao Liting , Du Lizhi , Qiu Yanguo 
Published: April 2023

A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor. After medium optimization, laccase activity increased by 12.77-fold compared to that without GHK-Cu. The laccase production of 1113.8 U L−1 was obtained by scaling-up culture in 5-L stirring tank. The laccase production induced by CuSO4 was poorer than that of GHK-Cu at the same mole concentration. GHK-Cu could increase the permeability of cell membrane with less damage, and it facilitated the adsorption, accumulation, and utilization of copper by fungal cells, which was beneficial for laccase synthesis. GHK-Cu induced better expression of laccase related genes than that of CuSO4, resulting in higher laccase production. This study provided a useful method for induced production of laccase by applying GHK chelated metal ion as a non-toxic inducer, which reduced the safety risk of laccase broth and provided the potential application of crude laccase in food industry. In addition, GHK can be used as the carrier of different metal ions to enhance the production of other metalloenzymes.
Binary and ternary complexes of epinephrine with alginate and biologically and environmentally relevant metal cations
Authors: Irto Anna , Cigala Rosalia Maria , Alessandrello Chiara , De Stefano Concetta , Gattuso Giuseppe , Crea Francesco 
Published: April 2023

The speciation of epinephrine (Eph−) in the presence of alginate (Alg2-) and two biological and environmental relevant metal cations (Cu2+, UO22+) was investigated at T = 298.15K, I = 0.15–1.00 mol dm−3 in NaCl(aq). The formation of binary and ternary complexes was evaluated and, since epinephrine can behave as a zwitterion, the Eph−/Alg2- interaction was studied by means of DOSY NMR. The dependence of the equilibrium constants on ionic strength was studied using an extended Debye-Hückel type equation and the SIT approach. The effect of temperature was investigated by means of isoperibolic titration calorimetry: the entropic contribution was the driving force for the Cu2+/Eph− complexes formation. The sequestering ability of Eph− and Alg2- on Cu2+, evaluated by the pL0.5 calculation, increased with pH and ionic strength. The determination of pM parameter showed that Eph− had a higher Cu2+ affinity with respect to Alg2-. The formation of Eph−/Alg2- species was also investigated by UV-Vis spectrophotometry and 1H NMR measurements. The ternary Cu2+/Eph−/Alg2- and Cu2+/UO22+/Eph− interactions were also studied. The “extra-stability” calculated for the mixed ternary species confirmed that their formation was thermodynamically favorable.
A genetic and developmental biological approach for a family with complex congenital heart diseases—evidence of digenic inheritance
Authors: Yoshida Yu , Uchida Keiko , Kodo Kazuki , Ishizaki-Asami Reina , Maeda Jun , Katsumata Yoshinori , Yuasa Shinsuke , Fukuda Keiichi , Kosaki Kenjiro , Watanabe Yusuke , Nakagawa Osamu , Yamagishi Hiroyuki 
Published: April 2023

ObjectiveCongenital heart disease (CHD) is caused by cardiovascular developmental defects and has a global prevalence of ∼1%. The etiology of CHD is multifactorial and remains generally unknown, despite advances in analytical techniques based on next-generation sequencing (NGS). The aim of our study was to elucidate the multi-genetic origin and pathogenesis of an intriguing familial case with complex CHD.MethodsWe performed an original trio-based gene panel analysis using NGS of the family, including two siblings with CHD of single ventricular phenotype, and their unaffected parents. The pathogenicity of the detected rare variants was investigated in silico, and the functional effects of the variants were confirmed in vitro using luciferase assays. The combinatorial effect of gene alterations of the putative responsible genes was tested in vivo using genetically engineered mutant mice.ResultsNGS-based gene panel analyses revealed two heterozygous rare variants in NODAL and in TBX20 common to the siblings and to just one of parents. Both variants were suspected pathogenic in silico, and decreased transcriptional activities of downstream signaling pathways were observed in vitro. The analyses of Nodal and Tbx20 double mutant mice demonstrated that Nodal+/−Tbx20−/− embryos showed more severe defects than Nodal+/+Tbx20−/− embryos during early heart development. The expression of Pitx2, a known downstream target of Nodal, was downregulated in Tbx20−/− mutants.ConclusionsTwo rare variants on NODAL and TBX20 genes detected in this family were considered to be loss-of-function mutations. Our results suggest that NODAL and TBX20 may be complementary for the cardiac development, and a combinatorial loss-of-function of NODAL and TBX20 could be implicated in digenic inherence as the etiology of complex CHD associated with single ventricle defects in this family.
Macrophages-derived Factor XIII links coagulation to inflammation in COPD
Authors: Bazzan Erica , Casara Alvise , Radu Claudia Maria , Tinè Mariaenrica , Biondini Davide , Faccioli Eleonora , Pezzuto Federica , Bernardinello Nicol , Conti Maria , Balestro Elisabetta , Calabrese Fiorella , Simioni Paolo , Rea Federico , Turato Graziella , Spagnolo Paolo , Cosio Manuel G. , Saetta Marina 
Published: April 2023

BackgroundThe local, extravascular, activation of the coagulation system in response to injury is a key factor mediating the resulting inflammatory response. Coagulation Factor XIIIA (FXIIIA) found in alveolar macrophages (AM) and dendritic cells (DC), by influencing fibrin stability, might be an inflammatory modifier in COPD.AimsTo study the expression of FXIIIA in AM and Langerin+DC (DC-1) and their relation to the inflammatory response and disease progression in COPD.MethodsIn 47 surgical lungs, 36 from smokers (22 COPD and 14 no-COPD) and 11 from non-smokers we quantified by immunohistochemistry FXIIIA expression in AM and DC-1 along with numbers of CD8+Tcells and CXCR3 expression in lung parenchyma and airways. Lung function was measured prior to surgery.ResultsThe percentage of AM expressing FXIII (%FXIII+AM) was higher in COPD than no-COPD and non-smokers. DC-1 expressed FXIIIA and their numbers were higher in COPD than no-COPD and non-smokers. DC-1 positively correlated with %FXIII+AM (r=0.43; p&lt;0.018). CD8+Tcells, which were higher in COPD than in no-COPD, were correlated with DC-1 (p&lt;0.01) and %FXIII+AM. CXCR3+ cells were increased in COPD and correlated with %FXIII+AM (p&lt;0.05). Both %FXIII+AM (r=-0.6; p=0.001) and DC-1 (r=-0.7; p=0.001) correlated inversely with FEV1.ConclusionFXIIIA, an important link between the extravascular coagulation cascade and inflammatory response, is significantly expressed in alveolar macrophages and dendritic cells of smokers with COPD, suggesting that it could play an important role in the adaptive inflammatory reaction characteristic of the disease.
Epidemiological profile and risk factors associated with death in patients receiving invasive mechanical ventilation in an adult intensive care unit from Brazil: a retrospective study
Authors: Palamim Camila Vantini Capasso , Boschiero Matheus Negri , Marson Fernando Augusto Lima 
Published: April 2023

IntroductionUnderstanding the epidemiological profile and risk factors associated with invasive mechanical ventilation (IMV) is essential to manage the patients better and to improve health services. Therefore, our objective was to describe the epidemiological profile of adult patients in intensive care that required IMV in-hospital treatment. Also, to evaluate the risks associated with death and the influence of positive end-expiratory pressure (PEEP) and arterial oxygen pressure (PaO2) at admission in the clinical outcome.MethodsWe conducted an epidemiological study analyzing medical records of inpatients who received IMV from January 2016 to December 2019 prior to the Coronavirus Disease (COVID)-19 pandemic in Brazil. We considered the following characteristics in the statistical analysis: demographic data, diagnostic hypothesis, hospitalization data, and PEEP and PaO2 during IMV. We associated the patients’ features with the risk of death using a multivariate binary logistic regression analysis. We adopted an alpha error of 0.05.ResultsWe analyzed 1,443 medical records; out of those, 570 (39.5%) recorded the patients’ deaths. The binary logistic regression was significant in predicting the patients’ risk of death [X2(9) = 288.335; p &lt; 0.001]. Among predictors, the most significant in relation to death risk were: age [elderly ≥65 years old; OR = 2.226 (95%CI = 1.728–2.867)]; male sex (OR = 0.754; 95%CI = 0.593–0.959); sepsis diagnosis (OR = 1.961; 95%CI = 1.481–2.595); need for elective surgery (OR = 0.469; 95%CI = 0.362–0.608); the presence of cerebrovascular accident (OR = 2.304; 95%CI = 1.502–3.534); time of hospital care (OR = 0.946; 95%CI = 0.935–0.956); hypoxemia at admission (OR = 1.635; 95%CI = 1.024–2.611), and PEEP &gt;8 cmH2O at admission (OR = 2.153; 95%CI = 1.426–3.250).ConclusionThe death rate of the studied intensive care unit was equivalent to that of other similar units. Regarding risk predictors, several demographic and clinical characteristics were associated with enhanced mortality in intensive care unit patients under mechanical ventilation, such as diabetes mellitus, systemic arterial hypertension, and older age. The PEEP &gt;8 cmH2O at admission was also associated with increased mortality since this value is a marker of initially severe hypoxia.
Is stress related to itch in German students? Results of an online survey
Authors: Kiupel Stephanie , Kupfer Jörg , Kottlors Sophia , Gieler Uwe , Yosipovitch Gil , Schut Christina 
Published: April 2023

IntroductionGerman students report to be more stressed than the general population. Highly stressed students from other countries (United States, Australia, Saudi-Arabia) were found to have more skin symptoms, including itch, than lowly stressed students. The current study aimed to assess whether itch is associated with stress in a larger sample of German students.Methods838 students (3.2% of all invited students) took part in the questionnaire based study and filled in the Perceived Stress Questionnaire as well as a modified version of the Self-Reported Skin Questionnaire. Students were categorized into highly (HSS) and lowly stressed students (LSS) by determination of the 25th and 75th percentile.ResultsItch occurred significantly more often in HSS compared to LSS (OR = 3.41 (2.17–5.35)). In addition, itch intensity was significantly related to perceived stress.DiscussionThese findings not only highlight the importance of offering stress management trainings also to students in Germany in order to minimize itch, but also encourage future research on stress and itch in certain student subgroups.
Transcriptomic profiling revealed important roles of amino acid metabolism in fruiting body formation at different ripening times in Hypsizygus marmoreus
Authors: Xiang Quanju , Arshad Muhammad , Li Yakun , Zhang Huijuan , Gu Yunfu , Yu Xiumei , Zhao Ke , Ma Menggen , Zhang Lingzi , He Maolan , Chen Qiang 
Published: April 2023

IntroductionHypsizygus marmoreus is an industrial mushroom that is widely cultivated in East Asia. Its long postripening stage before fruiting severely limits its industrialized production.MethodsFive different mycelial ripening times (30, 50, 70, 90, and 100 d) were chosen and primordia (30P, 50P, 70P, 90P, and 110P) were collected for comparative transcriptomic analyses. The corresponding substrates (30F, 50F, 70F, 90F, and 110F) were used for nutrient content and enzyme activity determination.ResultsIn pairwise comparisons between 110P and other primordia, a total of 1,194, 977, 773, and 697 differentially expressed genes (DEGs) were identified in 30P_110P, 50P_110P, 70P_110P, and 90P_110P, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes Genomes (KEGG) functional enrichment analyses revealed that the DEGs were mainly associated with amino acid metabolism, and lipid and carbohydrate metabolism pathways. Tyrosine, tryptophan, phenylalanine and histidine metabolism were enriched in all groups. Among the main carbon nutrients, the contents of cellulose and hemicellulose were high, and the lignin content decreased with the extension of the ripening time. Laccase had the highest activity, and acid protease activity decreased with the extension of the ripening time.DiscussionThe highly enrichment for amino acid metabolic pathways in primordia reveals that these pathways are essential for fruiting body formation in H. marmoreus, and these results will provide a basis for the optimization of its cultivation.