bluuebunny/crossref_metadata_2025_split huggingface dataset:
An end-to-end pipeline based on open source deep learning tools for reliable analysis of complex 3D images of ovaries
Authors: Lesage Manon , Thomas Manon , Pécot Thierry , Ly Tu-Ky , Hinfray Nathalie , Beaudouin Remy , Neumann Michelle , Lovell-Badge Robin , Bugeon Jérôme , Thermes Violette 
Published: March 2023

ABSTRACTComputational analysis of bio-images by deep learning (DL) algorithms has made exceptional progress in recent years and has become much more accessible to non-specialists with the development of ready-to-use tools. The study of oogenesis mechanisms and female reproductive success has also recently benefited from the development of efficient protocols for three-dimensional (3D) imaging of ovaries. Such datasets have a great potential for generating new quantitative data but are, however, complex to analyze due to the lack of efficient workflows for 3D image analysis. Here, we have integrated two existing open-source DL tools, Noise2Void and Cellpose, into an analysis pipeline dedicated to 3D follicular content analysis, which is available on Fiji. Our pipeline was developed on larvae and adult medaka ovaries but was also successfully applied to different types of ovaries (trout, zebrafish and mouse). Image enhancement, Cellpose segmentation and post-processing of labels enabled automatic and accurate quantification of these 3D images, which exhibited irregular fluorescent staining, low autofluorescence signal or heterogeneous follicles sizes. In the future, this pipeline will be useful for extensive cellular phenotyping in fish or mammals for developmental or toxicology studies.
Fipronil affects cockroach behavior and olfactory memory
Authors: Rosa Maria Eduarda , Campos Letícia , Borges Bruna Trindade , Santos Sara , Barreto Yuri Correia , de Assis Denis Reis , Hyslop Stephen , de Souza Velci Queiroz , Vinadé Lúcia , Dal Belo Cháriston André 
Published: March 2023

ABSTRACT
Fipronil (Fpl), an insecticide belonging to the class of phenylpyrazoles, is associated with the widespread mortality of pollinator insects worldwide. Based on studies carried out on residual concentrations of Fpl commonly found in the environment, in this study, we evaluated the sublethal effects of Fpl on behavior and other neurophysiological parameters using the cockroach Nauphoeta cinerea as a biological model. Sublethal doses of Fpl (0.1–0.001 μg g−1) increased the time spent grooming and caused dose-dependent inhibition of exploratory activity, partial neuromuscular blockade in vivo and irreversible negative cardiac chronotropism. Fpl also disrupted learning and olfactory memory formation at all doses tested. These results provide the first evidence that short-term exposure to sublethal concentrations of Fpl can significantly disrupt insect behavior and physiology, including olfactory memory. These findings have implications for current pesticide risk assessment and could be potentially useful in establishing a correlation with pesticide effects in other insects, such as honey bees.
To escape or to pursue: opposite decision making concerning a single moving object is influenced by starvation and sex
Authors: Salido Carla A. , Gancedo Brian J. , Tomsic Daniel 
Published: March 2023

ABSTRACT
Decision-making processes in the context of prey–predator interactions are studied from the side of the prey or the predator. Thus, prey capture and escape behaviours are researched separately, using different stimuli in different species. The crab Neohelice preys upon individuals of its own species; hence, it behaves as prey and as predator. These two innate opposite behaviours can be elicited by the same object moving on the ground. Here, we studied how the decision to perform avoidance, predatory or freezing responses to a moving dummy depends on sex and starvation level. In the first experiment, we assessed the probability of each response type in unfed crabs for 22 days. Males showed a higher predatory response probability than females. When starvation increased, the predatory response increased, while avoidance and freezing declined, but this only occurred in males. In the second experiment, we compared regularly fed and unfed males for 17 days. While fed crabs did not change their behaviour throughout the experiment, unfed crabs significantly intensified their predatory responses, displayed different exploratory activities and pursued earlier than fed crabs. Our results show the unusual situation of an animal that, to deal with a single stimulus, has to choose between opposite innate behaviours. This is a value-based decision as it is affected by factors other than the stimulus itself.
Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects
Authors: Sama-ae Imran , Sangkanu Suthinee , Siyadatpanah Abolghasem , Norouzi Roghayeh , Chuprom Julalak , Mitsuwan Watcharapong , Surinkaew Sirirat , Boonhok Rachasak , Paul Alok K. , Mahboob Tooba , Abtahi Najme Sadat , Jimoh Tajudeen O. , Oliveira Sónia M.R. , Gupta Madhu , Sin Chea , de Lourdes Pereira Maria , Wilairatana Polrat , Wiart Christophe , Rahmatullah Mohammed , Dolma Karma G. , Nissapatorn Veeranoot 
Published: March 2023

Background: Propolis is a natural resinous mixture produced by bees. It provides beneficial effects on human health in the treatment/management of many diseases. The present study was performed to demonstrate the anti-Acanthamoeba activity of ethanolic extracts of Propolis samples from Iran. The interactions of the compounds and essential proteins of Acanthamoeba were also visualized through docking simulation. Methods: The minimal inhibitory concentrations (MICs) of Propolis extract against Acanthamoeba trophozoites and cysts was determined in vitro. In addition, two-fold dilutions of each of agents were tested for encystment, excystment and adhesion inhibitions. Three major compounds of Propolis extract such as chrysin, tectochrysin and pinocembrin have been selected in molecular docking approach to predict the compounds that might be responsible for encystment, excystment and adhesion inhibitions of A. castellanii. Furthermore, to confirm the docking results, molecular dynamics (MD) simulations were also carried out for the most promising two ligand-pocket complexes from docking studies. Results: The minimal inhibitory concentrations (MICs) 62.5 and 125 µg/mL of the most active Propolis extract were assessed in trophozoites stage of Acanthamoeba castellanii ATCC30010 and ATCC50739, respectively. At concentrations lower than their MICs values (1/16 MIC), Propolis extract revealed inhibition of encystation. However, at 1/2 MIC, it showed a potential inhibition of excystation and anti-adhesion. The molecular docking and dynamic simulation revealed the potential capability of Pinocembrin to form hydrogen bonds with A. castellanii Sir2 family protein (AcSir2), an encystation protein of high relevance for this process in Acanthamoeba. Conclusions: The results provided a candidate for the development of therapeutic drugs against Acanthamoeba infection. In vivo experiments and clinical trials are necessary to support this claim.
Central composite design driven optimization of sustainable stability indicating HPLC method for the determination of Tigecycline and greenness assessment
Authors: Hafez Hani Mohammed , Barghash Sona Soliman , Soliman Marwa M. , Soltan Moustafa K. , Abd Elrahman Mohamed , Katamesh Noha Salah 
Published: March 2023

Background: Tigecycline (TGC) is a recently developed antibiotic to battle resistant bacteria. The procedures outlined in the literature for analyzing TGC involve chemical solvents that could be hazardous. Therefore, this study aimed to create a sustainable and stable HPLC technique for quantifying Tigecycline in lyophilized powder. The powerful chemometric tool, experimental design (ED), will be applied to analyze the variables' interaction and impact on the selected analytical target profiles. Response surface methodology provides a tutorial on using the central composite design with three levels of variables and quadratic programming to optimize the design space of the developed method. Methods: The New HPLC method consisted of an aqueous buffer and ethanol as a green mobile phase run on a reversed-phase symmetry C18 column. A full resolution between the Tigecycline and its degradation product peaks was achieved in a short analytical runtime. Results: Further, the specificity, accuracy, precision, robustness and stability indicating power of the proposed approach were verified through stress degrading testing. Conclusions: Finally, the analytical eco-scale and the green Analytical Procedure Index (GAPI) were utilized to determine how environmentally friendly the recommended method was compared to other published approaches.
Unraveling the molecular mechanism of novel leukemia mutations on NTRK2 (A203T & R458G) and NTRK3 (E176D & L449F) genes using molecular dynamics simulations approach
Authors: M Al-Subaie Abeer , Kamaraj Balu , Ahmad Fazil , Alsamman Khaldoon 
Published: March 2023

Background: NTRK1, NTRK2, and NTRK3 are members of the neurotrophic receptor tyrosine kinases (NTRK) family, which encode TrkA, TrkB, and TrkC receptors, respectively. Hematologic cancers are also linked to point mutations in the NTRK gene's kinase domain. Trk fusions are the most common genetic change associated with oncogenic activity in Trk-driven liquid tumors. Thus, point mutations in NTRK genes may also play a role in tumorigenesis. The structural and functional effect of mutations in Trk-B & Trk-C proteins remains unclear. Methods: In this research, Homology (threading-based approach) modeling and the all-atom molecular dynamics simulations approaches are applied to examine the structural and functional behavior of native and mutant Trk-B and Trk-C proteins at the molecular level. Results: The result of this study reveals how the mutations in Trk-B (A203T & R458G) and Trk-C (E176D & L449F) proteins lost their stability and native conformations. The Trk-B mutant A203T became more flexible than the native protein, whereas the R458G mutation became more rigid than the native conformation of the Trk-B protein. Also, the Trk-C mutations (E176D & L449F) become more rigid compared to the native structure. Conclusions: This structural transition may interrupt the function of Trk-B and Trk-C proteins. Observing the impact of NTRK-2/3 gene alterations at the atomic level could aid in discovering a viable treatment for Trk-related leukemias.
Water-film thickness imaging based on time-multiplexed near-infrared absorption with up to 500  Hz repetition rate
Authors: Lubnow M. , Dreier T. , Schulz C. , Endres T. 
Published: March 2023

We demonstrate high-repetition-rate imaging of the liquid-film thickness in the 50–1000 µm range resulting from impinging water droplets on a glass surface. The pixel-by-pixel ratio of line-of-sight absorption at two time-multiplexed near-infrared wavelengths at 1440 and 1353 nm was detected with a high-frame-rate InGaAs focal-plane array camera. Frame rates of 1 kHz and thus measurement rates of 500 Hz could be achieved, well suited to capture the fast dynamics of droplet impingement and film formation. The droplets were sprayed onto the glass surface using an atomizer. Suitable absorption wavelength bands for water droplet/film imaging were determined from Fourier-transform infrared (FTIR) spectra of pure water between 298 and 338 K. At 1440 nm, the water absorption is nearly temperature-independent, making the measurements robust against temperature fluctuations. Time-resolved imaging measurements capturing the dynamics of the water droplet impingement and evolution were successfully demonstrated.
Stöd, hinder och förväntningar på läsaren
Authors: Sahlée Anna 
Published: March 2023

Support, Barriers, and Expectations of the Reader: On Textual and Visual Choices in a Picturebook Based on the Animated Feature Film Cars
Whenever an animated feature film aimed at children is released from a bigger production company, tie-ins will follow, including picturebooks. These books can be found in everyday grocery stores and have the potential to reach many children. Although discussed, few systematic descriptions of the literature in question exist. In this article, I examine textual and visual choices in the picturebook Bilar (Cars, 2015) based on the Disney & Pixar film with the same name (Lesseter). I investigate what barriers and what support it offers the reader to grip the story, but also whom the book addresses as a reader. The analysis focuses on resources important for comprehension, namely temporality and cohesion. Close-up analysis of text and pictures reveals and tries to explain temporal and logical gaps. The analysis shows that Bilar is quite complex, with features that contribute to distancing the story from the reader. The temporal unfolding advances quickly, but quite evenly, with few pauses. Leaps are drastic and linguistically unmarked, and intentions and events are presented indirectly. The text has few explicit causal ties and deviates from what is expected. Overall, the pictures repeat information in the text, but they also contradict the text in a way that suggests it was unintentional. Although the text manages to mediate the plot and intention – to the adult reader – it is hard to grasp in total without the movie in mind. The addressed reader, then, is plural: an adult and a child who have watched the movie and can fill the gaps, and who do not care too much about logical details. However, the study and the conclusion is based on a traditional view of the book and of reading. An investigation of the book in use might change or widen the understanding of this type of product.
Boric acid transport activity of marine teleost aquaporins expressed in Xenopus oocytes
Authors: Kumagai Shiori , Watanabe Erika , Hayashi Naoko , Kimura Yuuri , Kamiya Takehiro , Nagashima Ayumi , Ushio Kazutaka , Imaizumi Genki , Kim Juhyun , Munakata Keijiro , Umezawa Takahiro , Hirose Shigehisa , Kasai Koji , Fujiwara Toru , Romero Michael F. , Kato Akira 
Published: March 2023

AbstractMarine teleosts ingest large amounts of seawater containing various ions, including 0.4 mM boric acid, which can accumulate at toxic levels in the body. However, the molecular mechanisms by which marine teleosts absorb and excrete boric acid are not well understood. Aquaporins (Aqps) are homologous to the nodulin‐like intrinsic protein (NIP) family of plant boric acid channels. To investigate the potential roles of Aqps on boric acid transport across the plasma membrane in marine teleosts, we analyzed the function of Aqps of Japanese pufferfish (Takifugu rubripes) expressed in Xenopus laevis oocytes. Takifugu genome database contains 16 genes encoding the aquaporin family members (aqp0a, aqp0b, aqp1aa, aqp1ab, aqp3a, aqp4a, aqp7, aqp8bb, aqp9a, aqp9b, aqp10aa, aqp10bb, aqp11a, aqp11b, aqp12, and aqp14). When T. rubripes Aqps (TrAqps) were expressed in X. laevis oocytes, a swelling assay showed that boric acid permeability was significantly increased in oocytes expressing TrAqp3a, 7, 8bb, 9a, and 9b. The influx of boric acid into these oocytes was also confirmed by elemental quantification. Electrophysiological analysis using a pH microelectrode showed that these TrAqps increase B(OH)3 permeability. These results indicate that TrAqp3a, 7, 8bb, 9a, and 9b act as boric acid transport systems, likely as channels, in marine teleosts.
The Unlikely Continuity. The Communist Legacy of Romanian Consumer and Residential Credit Laws
Authors: Stănescu Cătălin Gabriel 
Published: March 2023

Abstract
The study challenges the dominant narrative that Romanian consumer and residential credit laws are a product of the country’s return to capitalism and the market economy, proposing an alternative interpretation. It demonstrates that the origins of Romanian consumer and residential credit laws lay in the pre-World War II and communist legislation that survived the 1989 Revolution. This shows that the communist legacy in consumer and residential credit is real and more enduring than expected and that communism did not differ from capitalism in this respect, other than how consumer and residential credit was organised. Using legal history and doctrinal analysis in a functional approach coupled with statistical and empirical data, the study proves the existence of a normative continuity between pre-and post-1989 Romanian consumer and residential credit laws that ended with the EU accession efforts. Finally, this continuity between communist and capitalist consumer and residential credit laws indicates functional similarities between the monarchic (capitalist) and communist legal systems, further undermining the relevance of political and economic ideology and the idea that communism was inimical to consumers and consumer or residential credit.
A partial inverse problem for non-self-adjoint Sturm–Liouville operators with a constant delay
Authors: Wang Yu Ping , Keskin Baki , Shieh Chung-Tsun 
Published: March 2023

Abstract
In this paper we study a partial inverse spectral problem for non-self-adjoint Sturm–Liouville operators with a constant delay and show that subspectra of two boundary value problems with one common boundary condition are sufficient to determine the complex potential. We developed the Horváth’s method in [M. Horváth,
On the inverse spectral theory of Schrödinger and Dirac operators,
Trans. Amer. Math. Soc. 353 2001, 10, 4155–4171] for the self-adjoint Sturm–Liouville operator without delay into the non-self-adjoint Sturm–Liouville differential operator with a constant delay.
Efficient employment of optical sources integrated with both light detectors and free space communication modulated channel under ambient conditions effects
Authors: Gopalan Anitha , Subramaniam Jayapoorani , Sangeetha Dharmapuri Patel , Ahammad Shaik Hasane , Hossain Md. Amzad , Rashed Ahmed Nabih Zaki 
Published: March 2023

Abstract
The paper simulates the efficient employment of light sources integrated with both light detectors and free space communication channel under ambient condition effects. The modified duobinary non return to zero line coding system based optical wireless communication channel under light or dense fog level effects. Max. Q factor against OWC reach is simulated at light and dense fog level for the suggested with the previous model. The study emphasis that the OWC length has been reached to 65 km at light fog level and the OWC length has been reached to 5 km at dense Fog level with acceptable signal power. The total light power and total electrical power are illustrated at light and dense fog levels. Max. Q factor and min. BER after receiver based OWC are clarified at light and dense fog levels.
Integrated optics based on electro optic modulators for long distance links and high bit rate communication
Authors: Rashed Ahmed Nabih Zaki , Kumar Ravichandran Prem , Gopinathan Charulatha , Rajendran Radhika , Ahammad Shaik Hasane , Hossain Md. Amzad 
Published: March 2023

Abstract
This paper has clarified the gallium aluminum arsenide and plastic modulators and its element circuit modeling design considerations for high signal processing distance links communications. Modulator circuit model design considerations and equations analysis are taken into account. Modulator source resistance, modulator inductance, and modulator capacitance based electro-optic modulator (EOM) is studied and analyzed against temperature variations. In addition to the switching voltage for EOM is analyzed at both 1300 nm and 1550 nm wavelength versus temperature variations. The operation efficiency and maximum modulation resonance frequency are studied for previous and proposed EOM versus modulator length variations. The lower modulator resistance, modulator capacitance, and modulator inductance can be achieved with the proposed EOM. As well as the proposed EOM has outlined the optimum operational efficiency and maximum resonance modulation frequency compared to the previous EOM.
How is an Illusion of Reason Possible? The Division of Nothing in the

Critique of Pure Reason
Authors: Smith Daniel James 
Published: March 2023

Abstract
This paper develops a new interpretation of the “table of nothing” that appears at the end of the transcendental aesthetic in the Critique of Pure Reason. In contrast to previous interpretations, which have taken it to be part of Kant’s account of the failures of reason, this paper argues that it should be understood as proffering Kant’s positive account of the objects he will be concerned with in the transcendental dialectic, namely objects that, properly understood, are nothing. I examine the four nothings in turn, showing how Kant’s concern is to develop a positive account of each one that allows him to determine its object while recognizing that it in some sense is not. I introduce Allison’s distinction between error and illusion and argue that the table of nothing is Kant’s theoretical account of what illusions are as objects, and thereby explains how something like a transcendental dialectic is possible at all.
“We are only to Appear to be Fighting Corruption…We can’t even Bite”: online memetic anti-corruption discourse in the Ghanaian media
Authors: Ofori Michael , Sena Dogbatse Felicity 
Published: March 2023

Abstract

Purpose
The goal of this study is to explore how memes are used as tools to expose and shame corruption in Ghana. The Ghanaian media has used memes to criticize unhealthy governmental and social practices and advocated for alternative approaches to addressing these issues. Thus, we explore the qualities memes possess in contributing to the anti-corruption discourse in Ghana.

Methods
Using visual rhetoric and metaphorical analysis of five media memes purposively collected from the Facebook page of Tilapia Da Cartoonist of TV3, this study discusses the approaches memes employ to ridicule and humorize the fight against corruption in Ghana and suggests remediations for governmental corruption.

Results
We find that memes play crucial anti-corruption roles. As such, they criticize corruption by showing the impact of corruption on the average citizen. Memes reveal the suppression of anti-corruption agents and agencies and the lack of real commitment to the fight against corruption in Ghana. While memes expose and criticize corruption, they also provide utilitarian approaches to addressing corruption. Central to this study, the research finds that humor is a basic feature of memes. However, within the Ghanaian media context, the humor in a meme does not affect the seriousness of the message a meme conveys.

Practical implications
Memes can be used strategically as an advocacy tool and a good complement to daily news reports.

Social implications
Media memes could be used as active media tools to produce, reproduce, distribute, and enhance complex socio-political narratives in society. This may have social and political implications for society.

Originality
This is the first study to explore memes as an anti-corruption media text. It is also the first study to examine the impact of humor on the message a meme conveys. This is done through a qualitative interpretation of the memes. Limitations and recommendations are provided.