bluuebunny/crossref_metadata_2025_split huggingface dataset:
A circumpolar parasite: Evidence of a cryptic undescribed species of sucking louse, Linognathus sp., collected from Arctic foxes, Vulpes lagopus, in Nunavut (Canada) and Svalbard (Norway)
Authors: Buhler Kayla J. , Snyman Louwtjie P. , Fuglei Eva , Davidson Rebecca , Ptochos Sokratis , Galloway Terry , Jenkins Emily 
Published: May 2023

AbstractThe North has experienced unprecedented rates of warming over the past few decades, impacting the survival and development of insects and the pathogens that they carry. Since 2019, Arctic foxes from Canada (Nunavut) have been observed with fur loss inconsistent with natural shedding of fur. Adult lice were collected from Arctic foxes from Nunavut (n = 1) and Svalbard (n = 2; Norway) and were identified as sucking lice (suborder Anoplura). Using conventional PCR targeting the mitochondrial cytochrome c oxidase subunit 1 gene (cox1), lice from Canada and Svalbard were 100% similar (8 pooled samples from Nunavut and 3 pooled samples from Svalbard), indicating that there is potential gene flow between ectoparasites on Scandinavian and North American Arctic fox populations. The cox1 sequences of Arctic fox lice and dog sucking lice (Linognathus setosus) had significant differences (87% identity), suggesting that foxes may harbour a cryptic species that has not previously been recognised. Conventional PCR targeting the gltA gene for Bartonella bacteria amplified DNA from an unknown gammaproteobacteria from two pooled louse samples collected from Svalbard foxes. The amplified sequences were 100% identical to each other but were only 78% like Proteus mirabilis reported in GenBank (CP053614), suggesting that lice on Arctic foxes may carry unique microorganisms that have yet to be described.
A group concept mapping and ethnographic study of intensive care rehabilitation culture
Authors: Wade Clare L. , Robinson Lisa J. , Baker Katherine , Saxton John M. , Wright Stephen E. , Adams Nicola , Scott Jason 
Published: May 2023

AbstractBackgroundEarly physical activity and physical rehabilitation are advocated in the critical care unit for patients recovering from critical illness. Despite this, there are still many factors associated with implementation of early physical rehabilitation into routine critical care and practice. One such factor that has been consistently identified is unit culture, yet there is little understanding of how or why the culture of a critical care unit impacts on implementation of early rehabilitation.AimTo develop a detailed understanding of the cultural barriers and enablers to the promotion and implementation of physical activity and early mobilization in National Health Service (NHS) critical care units in the United Kingdom (UK).Study DesignA mixed‐methods, two‐phase study incorporating online group concept mapping (GCM) and ethnography. GCM will be conducted to provide a multistakeholder co‐authored conceptual framework of rehabilitation culture. Ethnographic observations and interviews will be conducted of culture and behaviours in relation to the implementation and promotion of early physical activity and rehabilitation in two NHS critical care units in the North East of England.ResultsThe results of the Group Concept Mapping and ethnographic observations and interviews will be triangulated to develop a contextual framework of rehabilitation culture in critical care.Relevance to Clinical PracticeThis study will provide a detailed understanding of barriers and facilitators in relation to providing a positive rehabilitation culture in the critical care unit.
Arabidopsis thalianaB‐GATA factors repress starch synthesis and gravitropic growth responses
Authors: Sala Jan , Mosesso Niccolò , Isono Erika , Schwechheimer Claus 
Published: May 2023

Summary

Plants perceive the direction of gravity during skotomorphogenic growth, and of gravity and light during photomorphogenic growth. Gravity perception occurs through the sedimentation of starch granules in shoot endodermal and root columella cells. In this study, we demonstrate that the Arabidopsis thaliana GATA factors GNC (GATA, NITRATE‐INDUCIBLE, CARBON METABOLISM‐INVOLVED) and GNL/CGA1 (GNC‐LIKE/CYTOKININ‐RESPONSIVE GATA1) repress starch granule growth and amyloplast differentiation in endodermal cells.

In our comprehensive study, we analysed gravitropic responses in the shoot, root and hypocotyl. We performed an RNA‐seq analysis, used advanced microscopy techniques to examine starch granule size, number and morphology and quantified transitory starch degradation patterns. Using transmission electron microscopy, we examined amyloplast development.

Our results indicate that the altered gravitropic responses in hypocotyls, shoots and roots of gnc gnl mutants and GNL overexpressors are due to the differential accumulation of starch granules observed in the GATA genotypes. At the whole‐plant level, GNC and GNL play a more complex role in starch synthesis, degradation and starch granule initiation.

Our findings suggest that the light‐regulated GNC and GNL help balance phototropic and gravitropic growth responses after the transition from skotomorphogenesis to photomorphogenesis by repressing the growth of starch granules.
A child with CARD14‐associated papulosquamous eruption (CAPE) successfully treated with ustekinumab
Authors: Nogueira Miguel , Reis Joel , Abreu Maria , Coelho André , Reis Cláudia F. , Machado Susana 
Published: May 2023

AbstractCARD14‐associated papulosquamous eruption (CAPE) is a rare inflammatory skin eruption that can have features of psoriasis, pityriasis rubra pilaris, and erythroderma. This skin condition is known for its resistance to topical or conventional systemic therapies. Successful treatment of CAPE with anti‐IL‐12/IL‐23 and IL‐17 inhibitors has been reported. We present a case of a 2‐year‐old girl with CAPE who was successfully treated with ustekinumab.
Characterization and development of a plastid genome base editor, ptpTALECD
Authors: Nakazato Issei , Okuno Miki , Itoh Takehiko , Tsutsumi Nobuhiro , Arimura Shin‐ichi 
Published: May 2023

SUMMARYThe modification of photosynthesis‐related genes in plastid genomes may improve crop yields. Recently, we reported that a plastid‐targeting base editor named ptpTALECD, in which a cytidine deaminase DddA functions as the catalytic domain, can homoplasmically substitute a targeted C to T in plastid genomes of Arabidopsis thaliana. However, some target Cs were not substituted. In addition, although ptpTALECD could substitute Cs on the 3′ side of T and A, it was unclear whether it could also substitute Cs on the 3′ side of G and C. In this study, we identified the preferential positions of the substituted Cs in ptpTALECD‐targeting sequences in the Arabidopsis plastid genome. We also found that ptpTALECD could substitute Cs on the 3′ side of all four bases in plastid genomes of Arabidopsis. More recently, a base editor containing an improved version of DddA (DddA11) was reported to substitute Cs more efficiently, and to substitute Cs on the 3′ side of more varieties of bases in human mitochondrial genomes than a base editor containing DddA. Here, we also show that ptpTALECD_v2, in which a modified version of DddA11 functions as the catalytic domain, more frequently substituted Cs than ptpTALECD in the Arabidopsis plastid genome. We also found that ptpTALECD_v2 tended to substitute Cs at more positions than ptpTALECD. Our results reveal that ptpTALECD can cause a greater variety of codon changes and amino acid substitutions than previously thought, and that ptpTALECD and ptpTALECD_v2 are useful tools for the targeted base editing of plastid genomes.
Protein interactome of 3′,5′‐cAMP reveals its role in regulating the actin cytoskeleton
Authors: Figueroa Nicolás E. , Franz Peter , Luzarowski Marcin , Martinez‐Seidel Federico , Moreno Juan C. , Childs Dorothee , Ziemblicka Aleksandra , Sampathkumar Arun , Andersen Tonni Grube , Tsiavaliaris Georgios , Chodasiewicz Monika , Skirycz Aleksandra 
Published: May 2023

SUMMARYIdentification of protein interactors is ideally suited for the functional characterization of small molecules. 3′,5′‐cAMP is an evolutionary ancient signaling metabolite largely uncharacterized in plants. To tap into the physiological roles of 3′,5′‐cAMP, we used a chemo‐proteomics approach, thermal proteome profiling (TPP), for the unbiased identification of 3′,5′‐cAMP protein targets. TPP measures shifts in the protein thermal stability upon ligand binding. Comprehensive proteomics analysis yielded a list of 51 proteins significantly altered in their thermal stability upon incubation with 3′,5′‐cAMP. The list contained metabolic enzymes, ribosomal subunits, translation initiation factors, and proteins associated with the regulation of plant growth such as CELL DIVISION CYCLE 48. To functionally validate obtained results, we focused on the role of 3′,5′‐cAMP in regulating the actin cytoskeleton suggested by the presence of actin among the 51 identified proteins. 3′,5′‐cAMP supplementation affected actin organization by inducing actin‐bundling. Consistent with these results, the increase in 3′,5′‐cAMP levels, obtained either by feeding or by chemical modulation of 3′,5′‐cAMP metabolism, was sufficient to partially rescue the short hypocotyl phenotype of the actin2 actin7 mutant, severely compromised in actin level. The observed rescue was specific to 3′,5′‐cAMP, as demonstrated using a positional isomer 2′,3′‐cAMP, and true for the nanomolar 3′,5′‐cAMP concentrations reported for plant cells. In vitro characterization of the 3′,5′‐cAMP–actin pairing argues against a direct interaction between actin and 3′,5′‐cAMP. Alternative mechanisms by which 3′,5′‐cAMP would affect actin dynamics, such as by interfering with calcium signaling, are discussed. In summary, our work provides a specific resource, 3′,5′‐cAMP interactome, as well as functional insight into 3′,5′‐cAMP‐mediated regulation in plants.
Isotopically non‐stationary 13C metabolic flux analysis of two closely related fast‐growing cyanobacteria, Synechococcus elongatusPCC 11801 and 11802
Authors: Jaiswal Damini , Nenwani Minal , Wangikar Pramod P. 
Published: May 2023

SUMMARYSynechococcus elongatus PCC 11801 and 11802 are closely related cyanobacterial strains that are fast‐growing and tolerant to high light and temperature. These strains hold significant promise as chassis for photosynthetic production of chemicals from carbon dioxide. A detailed quantitative understanding of the central carbon pathways would be a reference for future metabolic engineering studies with these strains. We conducted isotopic non‐stationary 13C metabolic flux analysis to quantitively assess the metabolic potential of these two strains. This study highlights key similarities and differences in the central carbon flux distribution between these and other model/non‐model strains. The two strains demonstrated a higher Calvin–Benson–Bassham (CBB) cycle flux coupled with negligible flux through the oxidative pentose phosphate pathway and the photorespiratory pathway and lower anaplerosis fluxes under photoautotrophic conditions. Interestingly, PCC 11802 shows the highest CBB cycle and pyruvate kinase flux values among those reported in cyanobacteria. The unique tricarboxylic acid (TCA) cycle diversion in PCC 11801 makes it ideal for the large‐scale production of TCA cycle‐derived chemicals. Additionally, dynamic labeling transients were measured for intermediates of amino acid, nucleotide, and nucleotide sugar metabolism. Overall, this study provides the first detailed metabolic flux maps of S. elongatus PCC 11801 and 11802, which may aid metabolic engineering efforts in these strains.
Evaluation of the Rio de Janeiro State Flood Warning System: A Case Study for the Hydrographic Region of the Médio Paraíba do Sul (RJ), Brazil
Authors: De Pontes Lídia Luisa Mota , França José Ricardo De Almeida , De Carvalho Lino Augusto Sander 
Published: May 2023

This work is a case study for the Hydrographic Region of the Médio Paraíba do Sul located in the State of Rio de Janeiro (Brazil), which has natural disasters, such as floods, as the most recurrent. Due to the social, economic and environmental impacts that these disasters cause, this research aim to analyze the history of alerts issued by the Flood Warning System (FWS) and to assess its efficiency. Through the retro-analysis of Alert Trigger Events (ATE) that occurred in the monitored rivers, investigations were also carried out in the hydrological and meteorological scope. The results identified that the Attention stage belonging to the operational protocol significantly reduces the efficiency of the FWS about sending alerts, causing high false alarm rate. Regarding the influence that variation in the type of data transmission has on the Operational Protocol in RH-III, it is considered that the time interval between data transmission and their availability on the State Environmental Institute (SEI) erver generates a false alarm, however the FWS is still efficient. Barra Mansa River is the only one that has flood events, which raises questions about the representativeness of the other stations spread across RH-III. In addition, 60% of alerts issued for RH-III were related to episodes of Cold Fronts and South Atlantic Convergence Zones. These information helps improve the description of the flood events in the region. Rainfall Anomaly Index (RAI) identified periods that the rainfall rate was below the climatological average, indicating a drier environment, but with alerts issued.
A Nonlinear Repeated Impact Model of Auxetic Honeycomb Structures Considering Geometric Nonlinearity and Tensile/Compressive Deformation
Authors: Liu Yunfei , Qin Zhaoye , Chu Fulei 
Published: May 2023

Abstract
This study aims to improve the impact protection performance of composite structures by combining a honeycomb core with negative Poisson’s ratio and graphene platelets reinforced (GPR) face sheets. The paper investigates the nonlinear repeated low-velocity impact responses of auxetic honeycomb composite plates, taking into account loading-unloading-reloading processes. Effective material properties of the auxetic honeycomb core and GPR face sheets are obtained by using the proposed modified Gibson function and Halpin–Tsai model. Then, taking into account geometric nonlinearity, the nonlinear equations of motion for the system were derived by Hamilton's principle. Afterward, the time-varying contact force between the composite plate and a spherical impactor is defined by the modified nonlinear Hertz contact theory. The Galerkin method and variable-step Runge–Kutta algorithm are selected to obtain nonlinear impact responses. The proposed methods are verified by finite element simulation and experiment. Finally, the study evaluates the effects of key parameters on the nonlinear repeated low-velocity impact responses.
Feature Extraction Based on Self-Supervised Learning for Remaining Useful Life Prediction
Authors: Yu Zhenjun , Lei Ningbo , Mo Yu , Xu Xin , Li Xiu , Huang Biqing 
Published: May 2023

AbstractThe prediction of the remaining useful life (RUL) is of great significance to ensure the safe operation of industrial equipment and to reduce the cost of regular preventive maintenance. However, the complex operating conditions and various fault modes make it difficult to extract features containing more degradation information with existing prediction methods. We propose a self-supervised learning method based on variational automatic encoder (VAE) to extract features of data’s operating conditions and fault modes. Then the clustering algorithm is applied to the extracted features to divide data from different failure modes into different categories and reduce the impact of complex working conditions and fault modes on the estimation accuracy. In order to verify the effectiveness of the proposed method, we conduct experiments with different network structures on the C-MAPSS dataset, and the results verified that our method can effectively improve the feature extraction capability of the model. In addition, the experimental results further demonstrate the superiority and necessity of using hidden features for clustering rather than raw data.
Evaluation of Contrived Wear Methodology in End Milling of Inconel 718
Authors: Potthoff Nils , Agarwal Ankit , Wöste Florian , Wiederkehr Petra , Mears Laine 
Published: May 2023

Abstract
Tool wear plays a decisive role in achieving the required surface quality and dimensional accuracy during the machining of Inconel 718-based products. The highly stochastic phenomenon of tool wear, particularly in later stages, results in difficulty in predicting the failure point of the tool. The present research work aims to study this late-stage wear of the tool by generating consistent wear conditions and thereby decoupling the late-stage wear from the wear history. To do so, a multi-axis grinding operation is employed to create artificial tool wear that replicates the topology of natural wear occurring in the process. In order to evaluate the imitating ability of the proposed methodology, microscopic images in different wear states of naturally and contrived worn tools were analyzed. The methodology was validated by comparing the resulting process forces measured during end milling with the natural and contrived worn tool for different path strategies. Finally, a qualitative finite element (FE) analysis was conducted, and specific force coefficients for worn tool segments were determined through simulation.
Code Verification for the SENSEI CFD Code
Authors: Xue Weicheng , Wang Hongyu , Roy Christopher J. 
Published: May 2023

Abstract
This work performs systematic studies for code verification for turbulence modeling in our research CFD code SENSEI. Turbulence modeling verification cases including cross term sinusoidal manufactured solutions and a few exact solutions are used to justify the proper Spalart–Allmaras and Menter’s SST turbulence modeling implementation of the SENSEI CFD code. The observed order of accuracy matches fairly well with the formal order for both the 2D/3D steady-state and 2D unsteady flows when using the cross term sinusoidal manufactured solutions. This work concludes that it is important to keep the spatial discretization error in a similar order of magnitude as the temporal error in order to avoid erroneous analysis when performing combined spatial and temporal order analysis. Since explicit time marching scheme typically requires smaller time-step size compared to implicit time marching schemes due to stability constraints, multiple implicit schemes such as the singly diagonally implicit Runge–Kutta multistage scheme and three point backward scheme are used in our work to mitigate the stability constraints.
Supplemental Material: Controls on metal fertility of dioritic intrusions in the Laiwu district, North China craton: Insights from whole-rock geochemistry and mineral compositions
Authors: Li Si-Yuan , Li Jian-Wei , et al.  
Published: May 2023

<p>Table S1: Major and trace elements geochemistry of whole-rock samples from the Fe-mineralized and barren dioritic intrusions in the Laiwu district. Table S2: Sr-Nd isotopic compositions of whole-rock samples from the Fe-mineralized and barren plutons in the Laiwu district. Table S3: Major and trace element compositions of amphibole from the Fe-mineralized and barren plutons in the Laiwu district. Table S4: U-Pb age data and trace elements of zircon from the Fe-mineralized and barren plutons in the Laiwu district. Table S5: Major and trace elements of apatite from the Fe-mineralized and barren plutons in the Laiwu district. Figure S1: Hand specimens of diorites were collected from the Kuangshan (A), Jiaoyu (B), Jinniushan (C), and Tietonggou plutons (D). Figure S2: CL images of representative zircons from the Kuangshan (A), Jiaoyu (B), Jinniushan (C), and Tietonggou diorite (D). Scale bar is 100 μm. Figure S3: U-Pb Concordia diagrams of zircons from the Kuangshan (A), Jiaoyu (B), Jinniushan (C), and Tietonggou plutons (D). The insets in each diagram are the weighted mean 206Pb/238U age. Figure S4: Selected oxide element Harker’s plots for the Fe-mineralized and barren plutons 20 in the Laiwu district. </p>
Multimodal prehabilitation service for patients with colorectal cancer: the challenges of implementation
Authors: Boyle Henry , Fullbrook Aidan , Wills Alasdair , Veal Isla , Peat Nicola , Al-Noor Zainab , Bradshaw Rebecca , Raga Arlene , Hegarty Aoife , Hainsworth Alison , Ilyas Minahi , Banugo Pele , Bidd Heena 
Published: May 2023

Prehabilitation has been shown to improve outcomes for patients undergoing major surgery; benefits include reductions in length of hospital stay and postoperative complications. Multimodal prehabilitation programmes lead to improved patient engagement and experience. This report describes implementation of a personalised multimodal prehabilitation programme for patients awaiting colorectal cancer surgery. We aim to highlight the successes, challenges and future direction of our programme.Patients listed for colorectal cancer surgery were referred for initial prehabilitation assessment. The prehabilitation group were assessed by specialist physiotherapists, dieticians and psychologists. An individualised programme was developed for each patient, aiming to optimise preoperative functional capacity and enhance physical and psychological resilience. Clinical primary outcome measures were recorded and compared with contemporaneous controls. For those undergoing prehabilitation, a set of secondary functional, nutritional and psychological outcomes were recorded at initial assessment and on completion of the programme.61 patients were enrolled in the programme from December 2021 to October 2022. 12 patients were excluded as they received less than 14 days prehabilitation or had incomplete data. The remaining 49 patients received a median duration of 24 days prehabilitation (range 15–91 days). The results show statistically significant improvements in the following functional outcome measures after prehabilitation: Rockwood scores, maximal inspiratory pressures, International Physical Activity Questionnaire Score and Functional Assessment of Chronic Illness - Fatigue Score. There was a lower postoperative complication rate in the prehabilitation group when compared with a control group (50% vs 67%).This quality improvement project has 3 Plan–Do–Study–Act (PDSA) cycles. PDSA 1 demonstrates prehabilitation can be successfully imbedded within a colorectal surgical unit and that patients are grateful for the service. PDSA 2 provides the project’s first complete data set and demonstrates functional improvements in patients undergoing prehabilitation. The third PDSA cycle is ongoing and aims to refine the prehabilitation interventions and improve clinical outcomes for patients undergoing colorectal cancer surgery.