bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome dataset from Solanum lycopersicum L. cv. Micro-Tom; wild type and two mutants of INDOLE-ACETIC-ACID (SlIAA9) using long-reads sequencing oxford nanopore technologies
Authors: Lubis Wahyu Muhammad Yuha , Adrian M , Jadid Nurul , Widiastuti Ani , Ezura Hiroshi , Mubarok Syariful , Hapsari Dhika Prita , Poerwanto Roedhy , Matra Deden Derajat 
Published: March 2023

Abstract
Objective
Tomatoes are the most widely consumed fruit vegetable and are relatively easy to cultivate. However, an increase in temperature causes some plants to respond with a decrease in fruit production. So, it is necessary to develop plants resistant to extreme temperature changes. The tomato cv. Micro-Tom has genetic variations in the gene of INDOLE-ACETIC-ACID, namely SlIAA9-3 and SlIAA9-5. However, the genetic information regarding the full-length transcript of the gene from this type of tomato plant is unknown. Therefore, this study aimed to determine the full-length transcript of the genes of these three types of tomatoes using long-reads sequencing technology from Oxford Nanopore.

Data description
The total RNA from three types of Micro-Tom was isolated with the RNeasy PowerPlant Kit. Then, the RNA sequencing process used PCR-cDNA Barcoding kit - SQK-PCB109 and continued with the processing of raw reads based on the protocol from microbepore protocol (https://github.com/felixgrunberger/microbepore). The resulting raw reads were 578 374, 409 905, and 851 948 for wildtype, iaa9-3, and iaa9-5, respectively. After obtaining cleaned reads, each sample was mapped to the tomato reference genome (S. lycopersicum ITAG4.0) with the Minimap2 program. In particular, 965 genes were expressed only in the iaa9-3 mutant, and 2332 genes were expressed only in the iaa9-5 mutant. Whereas in the wild type, 1536 genes are specifically expressed. In cluster analysis using the heatmap analysis, separate groups were obtained between the wild type and the two mutants. This proves an overall difference in transcript levels between the wild type and the mutants.
Blood pressure and dementia risk by physical frailty in the elderly: a nationwide cohort study
Authors: Cho Mi Hee , Han Kyungdo , Lee Seungwoo , Jeong Su-Min , Yoo Jung Eun , Kim SangYun , Lee Jinkook , Chun Sohyun , Shin Dong Wook 
Published: March 2023

Abstract
Background
Midlife hypertension has been recognized as a modifiable risk factor for dementia, but association between blood pressure (BP) in late life and dementia has been inconclusive. In addition, few studies have investigated effects of BP control on dementia incidence in the frail elderly. Thus, this study aimed to investigate the association of BP and dementia incidence with concomitant consideration of physical frailty in the young elderly population.

Methods
Using the Korean National Health Information Database, we identified 804,024 subjects without history of dementia at age 66. Dementia diagnosis was defined with prescription records of anti-dementia drugs and dementia-related diagnostic codes. Physical frailty was measured using the Timed Up and Go test. Association of BP and dementia incidence with concomitant consideration of physical frailty was investigated using Cox hazards analyses.

Results
The risks of Alzheimer’s and vascular dementia increased from systolic BP ≥ 160 and 130–139 mmHg, respectively; a significant association of dementia incidence with low BP was not observed. In the analyses stratified by the physical frailty status, low BP was not associated with increased risks of dementia within the groups both with and without physical frailty.

Conclusions
High BP was associated with increased risks of dementia, especially for vascular dementia, while low BP was not associated with increased risks of any type of dementia in young elderly people, even in those with physical frailty. This study suggests the need for tight BP control in young elderly people, irrespective of frailty status, to prevent dementia and supports the current clinical guidelines of hypertension treatment.
Decision-making regarding immediate vs. interval postpartum levonorgestrel intrauterine device insertion timing
Authors: Davenport Abigail , Morello Laura , Arora Kavita Shah 
Published: March 2023

Abstract
Background
Understanding decision-making for contraception initiation timing postpartum may help guide patients in selecting a contraceptive method most aligned with their reproductive goals. The objective of this study was to explore the decision-making process in patients who chose immediate postplacental (IPP) levonorgestrel intrauterine device (LNG IUD) insertion versus interval insertion at the postpartum follow-up visit.

Methods
We recruited English-speaking, reproductive-aged adult postpartum participants who received either an IPP or interval postpartum LNG IUD from September to December 2017 at MetroHealth Medical Center. We conducted interviews over the phone utilizing a pilot-tested, semi-structured interview guide. Interview topics included past experiences with contraception, provider counseling, intrapartum factors, and current experiences after IUD insertion.

Results
We interviewed 20 participants (12 IPP and eight interval IUD recipients). Participants receiving an IPP IUD described convenience, desire for immediate contraception, pain control and availability of alternative contraception options as influential for their decision. Patients who received an interval IUD performed outside research, focused on the events surrounding delivery, and generally favored additional recovery time before obtaining an IUD. Patients who received interval IUDs were often not aware that IPP IUDs were available. Early, frequent, and comprehensive counseling was viewed favorably when compared to counseling upon arrival to the laboring unit. While overall there was congruence of participant expectations and experiences, unexpected expulsion affected desire for future IUD use in some participants.

Conclusion
Providers should be mindful that prior experience and knowledge as well as delivery room considerations affect insertion timing decision-making.

Trial registration
N/A
Isolated Hepatic Perfusion With Melphalan for Patients With Isolated Uveal Melanoma Liver Metastases: A Multicenter, Randomized, Open-Label, Phase III Trial (the SCANDIUM Trial)
Authors: Olofsson Bagge Roger , Nelson Axel , Shafazand Amir , All-Eriksson Charlotta , Cahlin Christian , Elander Nils , Helgadottir Hildur , Kiilgaard Jens Folke , Kinhult Sara , Ljuslinder Ingrid , Mattsson Jan , Rizell Magnus , Sternby Eilard Malin , Ullenhag Gustav J. , Nilsson Jonas A. , Ny Lars , Lindnér Per 
Published: March 2023

PURPOSE About half of patients with metastatic uveal melanoma present with isolated liver metastasis, in whom the median survival is 6-12 months. The few systemic treatment options available only moderately prolong survival. Isolated hepatic perfusion (IHP) with melphalan is a regional treatment option, but prospective efficacy and safety data are lacking. METHODS In this multicenter, randomized, open-label, phase III trial, patients with previously untreated isolated liver metastases from uveal melanoma were randomly assigned to receive a one-time treatment with IHP with melphalan or best alternative care (control group). The primary end point was overall survival at 24 months. Here, we report the secondary outcomes of response according to RECIST 1.1 criteria, progression-free survival (PFS), hepatic PFS (hPFS), and safety. RESULTS Ninety-three patients were randomly assigned, and 87 patients were assigned to either IHP (n = 43) or a control group receiving the investigator's choice of treatment (n = 44). In the control group, 49% received chemotherapy, 39% immune checkpoint inhibitors, and 9% locoregional treatment other than IHP. In an intention-to-treat analysis, the overall response rates (ORRs) were 40% versus 4.5% in the IHP and control groups, respectively ( P < .0001). The median PFS was 7.4 months versus 3.3 months ( P < .0001), with a hazard ratio of 0.21 (95% CI, 0.12 to 0.36), and the median hPFS was 9.1 months versus 3.3 months ( P < .0001), both favoring the IHP arm. There were 11 treatment-related serious adverse events in the IHP group compared with seven in the control group. There was one treatment-related death in the IHP group. CONCLUSION IHP treatment resulted in superior ORR, hPFS, and PFS compared with best alternative care in previously untreated patients with isolated liver metastases from primary uveal melanoma.
The Colocation Model in Community Cancer Care: A Description of Patient Clinical and Demographic Attributes and Referral Pathways
Authors: Stockman Leah S. , Gundersen Daniel A. , Gikandi Ajami , Akindele Ruth N. , Svoboda Ludmila , Pohl Sarah , Drews Mark R. , Lathan Christopher S. 
Published: March 2023

PURPOSE: Cancer disparities are well documented among Black, Indigenous, and People of Color, yet little is known about the characteristics of programs that serve these populations. Integrating specialized cancer care services within community settings is important for addressing the needs of historically marginalized populations. Our National Cancer Institute–Designated Cancer Center initiated a clinical outreach program incorporating cancer diagnostic services and patient navigation within a Federally Qualified Health Center (FQHC) to expedite evaluation and resolution of potential cancer diagnoses with the goal of collaboration between oncology specialists and primary care providers in a historically marginalized community in Boston, MA. MATERIALS AND METHODS: Sociodemographic and clinical characteristics were analyzed from patients who were referred to the program for cancer-related care between January 2012 and July 2018. RESULTS: The majority of patients self-identified as Black (non-Hispanic) followed by Hispanic (Black and White). Twenty-two percent of patients had a cancer diagnosis. Treatment and surveillance plans were established for those with and without cancer at a median time to diagnostic resolution of 12 and 28 days, respectively. The majority of patients presented with comorbid health conditions. There was a high prevalence of self-reported financial distress among patients seeking care through this program. CONCLUSION: These findings highlight the wide spectrum of cancer care concerns in historically marginalized communities. This review of the program suggests that integrating cancer evaluation services within community-based primary health care settings offers promise for enhancing the coordination and delivery of cancer diagnostic services among historically marginalized populations and could be a method to address clinical access disparities.
Use of non-intrusive laser exfoliation to improve substance uptake into citrus leaves
Authors: Ponce Cabrera Luis , Etxeberria Ed , Gonzalez Pedro , Flores Reyes Teresa 
Published: March 2023

Background: Despite the presence of stomata in leaves, foliar application of agrochemicals can be extremely inefficient due to the low permeability of leaf cuticular surfaces to polar compounds. Methods: This study introduced a laser-based “wax exfoliation” method to facilitate the penetration of substances into the leaf and, together with enhancing their uptake into the phloem and subsequent transport across tissue. This investigation demonstrated the effectiveness and non-invasive properties of laser exfoliation to improve the penetration of foliar-applied substances into citrus leaves. Results: This work presents the use of laser energy to exfoliate the cuticle of a leaf, with the highest energy density of 0.76 J/ cm2 resulting in 85-90% exfoliation across the entire laser-spot area. The infrared wavelength of the erbium laser is specifically chosen to target the wax cuticle without causing damage to the underlying epidermal cells. This selective ablation allows for increased penetration of therapeutic compounds into the leaf and transportation throughout the plant's vasculature. This is demonstrated using a fluorescent glucose analog applied to the laser treated leaves, showing increased penetration and transport throughout the leaf. Conclusions: Our findings demonstrate that the use of laser technology for the foliar application of agrochemicals provides significant advantages, including improved foliage uptake of therapeutic compounds. The method of cuticle exfoliation presented in this study is highly effective and non-intrusive, limiting its effects to the cuticle only. Future work should focus on the development of prototypes for in-field applications, including testing at longer distances as the Er:YAG laser does not require a lens for this application.
Factors influencing uptake of postnatal care services at a maternal and child health clinic in Kajiado County Referral Hospital, Kenya
Authors: Kokedo Ruth Nailantei , Chepchirchir Angeline 
Published: March 2023

Background/Aims Despite birth aftercare recommendations that a postnatal visit should take place to examine both a woman and her child 6 weeks after birth, use of postnatal care services remains low at the Kajiado County referral hospital. This study's aim was to understand the factors influencing uptake of postnatal care among women of reproductive age. Methods This qualitative study used purposive sampling to select study respondents who were asked to attend focus group discussions to collect data. A total of 12 women were interviewed during their postnatal period. The qualitative data were analysed by theme categorisation. Results Respondents cited low income and forgetfulness, as well as the long distance to travel for postnatal services and long waiting times at the hospital as the main factors hindering attendance at and use of postnatal services. Conclusions Affordable and accessible postnatal care services need to be provided to follow up postnatal mothers, reducing the waiting time for services in postnatal clinics and ensuring women's partners support them to promote postnatal care service use.
Sexual and reproductive health rights and the use of reproductive health services among women in Ile-Ife, Nigeria
Authors: Ayamolowo Sunday Joseph , Aladegboye Mercy Kikelomo , Olowokere Adekemi Eunice , Bamidele Ifeoluwa Grace 
Published: March 2023

Background/Aims Reproductive health is an important part of a woman's general health and wellbeing. Being aware of one's sexual and reproductive health rights enables women to make informed decisions about and improve their sexual and reproductive health. This study assessed women's awareness of sexual and reproductive health rights and the use of sexual and reproductive health services in Ile-Ife, Nigeria. Methods A descriptive cross-sectional design was used for this study. Stratified random sampling was used to select 341 women of reproductive age in Ile-Ife. Data were collected with a structured questionnaire and analysed using descriptive and inferential statistics, with P<0.05 indicating significance. Results The majority (89.7%) of respondents had high awareness of sexual and reproductive health rights but reported poor use of sexual and reproductive health services (58.7%). The participants' age, marital status, occupation and distance to sexual and reproductive health facilities significantly influenced the uptake of sexual and reproductive health services. Conclusions Women in Ile-Ife were aware of their sexual and reproductive health rights but did not use sexual and reproductive health services. There is a need for health education to improve women's self-efficacy to use these services.
THE QUICKDASH AND PATIENT-RATED WRIST EVALUATION (PRWE) SCORES ARE NOT OPTIMAL PATIENT-REPORTED OUTCOME MEASURES FOLLOWING A FRACTURE OF THE DISTAL RADIUS DUE TO THE CEILING EFFECT: POTENTIAL IMPLICATIONS FOR FUTURE RESEARCH
Authors: Bell K.R. , Oliver W.M. , White T.O. , Molyneux S.G. , Clement N.D. , Duckworth A.D. 
Published: March 2023

The aims of this study in relation to distal radius fractures were to determine (1) the floor and ceiling effects for the QuickDASH and PRWE, (2) the floor and ceiling effects when defined to be within the minimal clinically important difference (MCID) of the minimal or maximal scores, (3) the degree to which patients with a floor or ceiling effect felt that their wrist was ‘normal’, and (4) patent factors associated with a floor or ceiling effect.A retrospective cohort study of patients sustaining a distal radius fracture during a single year was undertaken. Outcome measures included the QuickDASH, PRWE, EQ-5D-3L and normal wrist score.There were 526 patients with a mean age of 65yrs and 77% were female. Most patients were managed non-operatively (73%, n=385). The mean follow-up was 4.8yrs. A ceiling effect was observed for both the QuickDASH (22.3%) and PRWE (28.5%). When defined to be within the MCID of the best score, the effect increased to 62.8% for the QuickDASH and 60% for PRWE. Patients that achieved the best functional outcome according to the QuickDASH and PRWE felt their wrist was only 91% and 92% normal, respectively. Sex (p=0.000), age (p=0.000), dominant wrist injury (p=0.006 for QuickDASH and p=0.038 for PRWE), fracture type (p=0.015), and a better health-related quality of life (p=0.000) were independently associated with achieving a ceiling score.The QuickDASH and PRWE demonstrated ceiling effects following a distal radius fracture. Patients achieving ceiling scores did not consider their wrist to be ‘normal’ for them.
Risk factors of limited joint mobility in type 1 diabetic adolescents: a two-center experience in Iraq
Authors: Abdullah Wasnaa Hadi , Alabedi Rihab Faisal , Mussa Russul Feihan 
Published: March 2023

BACKGROUND Limited joint mobility (LJM) is the most common joint-related complications in patients with diabetes mellitus (DM) and indicates the presence of microvascular complications. This study aimed to assess the frequency of LJM among adolescents with type 1 DM (T1DM), its risk factors, and the other microangiopathies.
METHODS In this cross-sectional study, 75 patients (adolescents between 10 and 17 years old) with T1DM were assessed for hand joint mobility using a prayer sign test. It was carried out from January 15 to June 1, 2022 in Baghdad city, Iraq. The patients’ height, body mass index, blood pressure, glycated hemoglobin, and low-density lipoprotein (LDL) were recorded. Only 44 subjects were screened for diabetic nephropathy and 64 for diabetic retinopathy based on the eligibility criteria by the American Diabetes Association guidelines.
RESULTS Mean age was 13.60 (1.85) years, with a mean diabetes duration of 5.61 (2.87) years. LJM was found in 18 patients (24%). It was associated with a longer duration of diabetes (p<0.001), high LDL level (p = 0.012), diabetic nephropathy (p = 0.04), and diabetic retinopathy (p<0.001).
CONCLUSIONS The proportion of LJM was high among adolescents with T1DM, especially in those with a long duration of DM. It was associated with high LDL levels and diabetic microangiopathies (nephropathy and retinopathy).
La seguridad del visitante en Barbados: percepciones de los stakeholders
Authors: Griffin Clifford E. 
Published: March 2023

¿Es suficiente la información sobre la naturaleza, la ubicación y la incidencia de delitos contra los turistasvisitantes para desarrollar una política significativa de seguridad de los visitantes? ¿Las opiniones de los principales grupos interesados (los stakeholders) en el turismo son de utilidad para mejorar la política de seguridad de los visitantes y la sensación de seguridad? Para responder a estas preguntas, este estudio analiza 24 años de datos de delitos registrados cometidos contra los visitantes de Barbados y los datos de investigación de grupos de actores clave del turismo, y concluye: 1) que la información sobre la naturaleza, localización y la incidencia de los delitos contra los visitantes es necesario pero no suficiente para influir la política de seguridad de los visitantes, y 2) que las opiniones y aportaciones de las principales partes interesadas son esenciales para que los destinos serán más eficaces en la mejora de seguridad de los visitantes.
Diversity, Flexibility And Identity Mechanisms For Recycling Mature Tourist Destinations
Authors: Horrach Estarellas Biel 
Published: March 2023

La globalización de los recursos dinámicos está haciendo que las destinaciones turísticas, espacios cada vez más homogéneos, sean incapaces de responder a las nuevas motivaciones del turista y a las características específicas del territorio y el paisaje local. El espacio turístico ha sufrido cambios, resultado en ciudades sin estructura urbana que requieren nuevos patrones de planeamiento. A pesar de las predicciones, que determinaban un proceso en declive o un post-estancamiento, hay un gran potencial de reconversión de destinos turísticos obsoletos basados en sus características tipológicas y su interacción con el territorio.Los nuevos retos a los que se enfrenta la actividad turística: la diversidad de recursos turísticos, la flexibilidad en el viaje y la diferenciación del destino con respecto al resto mediante el impulso de su identidad propia, son metodológicamente tratadas a través de un estudio de las dinámicas turísticas regionales experimentadas en uno de los casos de desarrollo turístico costero más paradigmático, la isla de Mallorca.
Foresight as an Innovative Tool for Designing Tourist Destinations
Authors: Fernández Güell José Miguel 
Published: March 2023

La previsión es un campo de estudio relativamente nuevo que nació inicialmente para hacer previsiones de futuro en la ciencia y la tecnología, pero en el presente se usa cada vez más en asuntos territoriales. A pesar de que el uso de herramientas de previsión en el ámbito turístico ha sido limitado, hay una creciente necesidad de gestionar la incertidumbre que rodea el desarrollo turístico. Basándose en estas premisas, este artículo pretende probar la capacidad de las herramientas de previsión para anticipar los impactos de los complejos retos globales en el campo del turismo. Esta suposición es testada a través de un ejercicio de visión de futuro que explora la evolución de los segmentos de la demanda turística y sus implicaciones en el planeamiento de destinaciones turísticas. Dos grandes segmentos de demanda fueron visualizados para el horizonte 2020: “Demanda de nicho e innovación” y “Demanda masiva predecible”. Para ambos segmentos, se muestra el valor de la cadena de consumo turístico y se recomiendan guías de diseño espacial para destinos de sol y playa.
La sostenibilidad económica de la promoción y el fomento del turismo: el impuesto sobre las estancias en establecimientos turísticos (IEET) promovido por el Gobierno de Cataluña
Authors: Colom Oliva Mercè , Font Garolera Jaume , Imbert–Bouchard Ribera Daniel 
Published: March 2023

En un contexto de crisis económica general y déficit presupuestario, en 2012 el Gobierno de la Generalitat de Catalunya -uno de los primeros destinos turísticos de Europa y de la región mediterránea- creó el Impuesto sobre las Estancias en Establecimientos Turísticos (IEET) conocido popularmente como "tasa turística". Este impuesto tenía como finalidad paliar dicho déficit y disponer de un Fondo para el Fomento del Turismo (FFT) que permitiera mantener las inversiones en fomento y promoción turística.El presente artículo realiza un balance de la recaudación de los dos primeros ejercicios completos (años 2013 y 2014) de vigencia del impuesto y se valoran las acciones realizadas con los fondos obtenidos, especialmente en el uso de la TIC, tanto en las campañas de promoción como en acciones de fomento del turismo. Entre las principales conclusiones del estudio se constata que la entrada en vigor del impuesto ha permitido que los destinos beneficiarios incentivaran la creación y promoción de nuevos productos turísticos y el uso de nuevas herramientas de planificación y gestión turística, más eficientes y mejor adaptadas a las necesidades de la demanda turística actual.