bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photochemically Mediated Toluene Oxidation through a Copper Complex
Authors: Noß Christian , Göttlich Richard , Schindler Siegfried 
Published: May 2023

AbstractA method is described to photochemically oxidize toluene selectively to benzaldehyde, an essential compound in the chemical industry. Copper(I) complexes with different ligands were applied in combination with [Ru(bipy)3](PF6)2 and dioxygen as the oxidant. As a result, a “dioxygen adduct” copper complex, for example, a peroxido complex, is formed as the active species. The copper(II) complex obtained after oxidation can be photochemically reduced to the starting copper(I) species, and the process can be repeated continuously. The ligand tris(2‐methylpyridyl)amine (tmpa) led to the highest conversion rates.
Stable Spiro‐Fused Diarylaminyl Radicals: A New Type of a Neutral Mixed‐Valence System
Authors: Sentyurin Vyacheslav V. , Levitskiy Oleg A. , Bogdanov Alexey V. , Yankova Tatiana S. , Dorofeev Sergey G. , Lyssenko Konstantin A. , Gontcharenko Victoria E. , Magdesieva Tatiana V. 
Published: May 2023

AbstractA new type of neutral mixed‐valence system was synthesized using a facile one‐pot procedure. The spiro‐conjugated framework is additionally “fastened” with a biphenyl bridge, which does not directly participate in spin delocalization but makes the molecule stable and influences the reorganization energy and the energy barrier of the intramolecular electron transfer. The in‐depth experimental and quantum‐chemical study allowed determining the radicals as the Class II Robin‐Day‐mixed‐valence systems. The structure of the radicals was confirmed by the X‐ray data, which are relatively rare for Class II MV molecules. Advanced properties of the radicals, such as an ambipolar redox behavior and panchromatic absorption in the visible and NIR regions, along with their stability, make them of interest for materials science. All radicals demonstrate the SOMO‐HOMO inversion phenomenon, which was supported by the DFT and the experimental study.
Four out of five buprenorphine patients drop out: Study
Authors: Knopf Alison 
Published: May 2023

Only one in 5 patients started on buprenorphine treatment were retained for at least 180 days from January 2016 through October 2022, a recent study has found. During that same period, the monthly initiation rate at first increased, and then flattened (prior to the COVID‐19 epidemic). The study, Trends in Buprenorphine Initiation and Retention in the United States, 2016–2022, published in JAMA on April 25, is based on the IQVIA Longitudinal Prescription Database, which covers more than 90% of all prescriptions to pharmacies.
Future exposure of forest ecosystems to multi‐year drought in the United States
Authors: Costanza Jennifer K. , Koch Frank H. , Reeves Matthew C. 
Published: May 2023

AbstractAs the future climate becomes hotter or drier, forests may be exposed to more frequent or severe droughts. To inform efforts to ensure resilient forests, it is critical to know which forests may be most exposed to future drought and where. Longer duration droughts lasting 2–3 years or more are especially important to quantify because forests are likely to experience impacts. We summarized exposure to 36‐month drought for forests across the conterminous United States using the Standardized Precipitation‐Evapotranspiration Index (SPEI) overlaid on forest inventory plot locations. Exposure was quantified under 10 scenarios that combined five modeled climates and two Representative Concentration Pathways (RCPs, 4.5 and 8.5) through 2070. Future projections indicate a tripling of the monthly spatial extent of forests exposed to severe or extreme drought—38% of forests were exposed on average by mid‐century as opposed to 11% during 1991–2020 (2041–2070). Increases in drought exposure were greatest under hotter (HadGEM2‐ES), drier (IPSL‐CM5A‐MR), and middle (NorESM1‐M) climate models, under either RCP. Projections agreed that forests in portions of the western United States, especially the southwestern United States, could face high levels of exposure. Forest types including pinyon/juniper, woodland hardwoods, and ponderosa pine were projected to be exposed to drought more than 50% of the time on average across all scenarios by mid‐century, when no forest type was exposed more than 25% of the time under any scenario during the recent period. Projections agreed less for the eastern United States, but in some scenarios, particularly under RCP 8.5, large portions of the East could be exposed to drought nearly as often as parts of the West. Moreover, a substantial portion of oak/hickory forests occur in eastern regions, where projections agree on increased drought exposure. This study provides novel insights about the changing conditions forests face in both the eastern and western United States. Our results can be combined with information about the sensitivities and adaptive capacities of forest ecosystems to prioritize drought adaptation efforts.
Immunoassay based on peroxidase silver conjugate for the determination of human immunoglobulins against tick‐borne borreliosis (lyme disease) by voltammetry and spectrophotometry
Authors: Dorozhko Elena V. , Lunev Nikita A. , Korotkova Elena I. , Slepchenko Galina B. , Lipskikh Olga I. , Mikhnevich Ekaterina I. 
Published: May 2023

AbstractIn this work two strategies for the synthesis of peroxidase silver conjugates for the qualitative and quantitative determination of immunoglobulins (IgG) to ixodid tick‐borne borreliosis (ITBB) (Lyme disease) in human serum were proposed. The first approach for Ab‐HRP@AgNP conjugate synthesis involved silver nanoparticles (Ag NPs) capped with a commercial peroxidase conjugate (Ab‐HRP) by passive adsorption. The second strategy was based on the initial coupling of Ag NPs with human anti‐species antibodies (Ab) by passive adsorption followed by the introduction of horseradish peroxidase (HRP) enzyme into the reaction mixture as a blocking reagent for Ab@AgNP@HRP conjugate synthesis. The formation of peroxidase silver conjugates was proved by UV/Vis spectroscopy and Transmission Electron Microscopy (TEM). The catalytic activity of Ab‐HRP@AgNP and Ab@AgNP@HRP conjugates was evaluated by Michaelis‐Menten kinetics. A commercially available 96‐well microtiter plate with recombinant antigens to ITBB was used as a platform for immobilization of analyzed IgG. The HRP in Ab‐HRP@AgNP conjugate was found to retain a sufficient level of activity for interaction with the H2O2 substrate to form an intensely colored reaction product. Therefore Ab‐HRP@AgNP conjugate can be used in enzyme‐linked immunosorbent assay (ELISA) with spectrophotometric detection of 3,3’,5,5’‐Tetramethylbenzidine (TMB Ox) for quantitative determination of IgG to ITBB in human serum in the concentration range 12.5–800 ng ml−1 with LOD 2 ng ml−1. Ab@AgNP@HRP conjugate is recommended for the electrochemical determination of IgG to ITBB in human serum at LOD 3 ng ml−1 with registration of silver oxidation by linear sweep anodic stripping voltammetry (LSASV). Ag NPs in Ab‐HRP@AgNP and Ab@AgNP@HRP conjugates do not change electrochemical activity during storage and can be used as an electrochemical label in LSASV method in case of HRP inactivation. The immunoassay based on peroxidase silver conjugates expands the analytical potential for the determination of IgG to ITBB especially during the period of increasing incidence.
Development and Initial Testing of EcoToxChip, a Novel Toxicogenomics Tool for Environmental Management and Chemical Risk Assessment
Authors: Crump Doug , Hickey Gordon , Boulanger Emily , Masse Anita , Head Jessica A. , Hogan Natacha , Maguire Steve , Xia Jianguo , Hecker Markus , Basu Niladri 
Published: May 2023

Abstract
New approach methods (NAMs) are increasingly important to help accelerate the pace of ecological risk assessment and offer more ethical, affordable, and efficient alternatives to traditional toxicity tests. In the present study, we describe the development, technical characterization, and initial testing of a toxicogenomics tool, EcoToxChip (384-well quantitative polymerase chain reaction [qPCR] array), to support chemical management and environmental monitoring for three laboratory model species—fathead minnow (Pimephales promelas), African clawed frog (Xenopus laevis), and Japanese quail (Coturnix japonica). Chip design, including gene selection, was informed by a diverse end-user group and quality control metrics (e.g., primer assay, reverse transcription, and PCR efficiency) performed well based on a priori established criteria. Correlation with RNA sequencing (seq) data provided additional confidence in this novel toxicogenomics tool. Although the present study represents an initial testing of only 24 EcoToxChips for each of the model species, the results provide increased confidence in the robustness/reproducibility of EcoToxChips for evaluating perturbations in gene expression associated with chemical exposure and thus, this NAM, combined with early-life stage toxicity testing, could augment current efforts for chemical prioritization and environmental management. Environ Toxicol Chem 2023;42:1763–1771. © 2023 SETAC
Forecasting realized volatility of Bitcoin: The informative role of price duration
Authors: Slim Skander , Tabche Ibrahim , Koubaa Yosra , Osman Mohamed , Karathanasopoulos Andreas 
Published: May 2023

AbstractMotivated by the relationship between trading intensity and volatility and the attractiveness of duration‐based volatility estimators, this paper investigates the ability of price duration to forecast realized volatility of Bitcoin. Using high‐frequency transaction data, trading intensity is measured by price duration and incorporated in the class of heterogeneous autoregressive (HAR) models. Results provide compelling evidence that trading intensity improves the forecasting performance of a highly competitive set of HAR models, commonly used in the literature. HAR extensions that incorporate price duration systematically deliver the lowest forecast errors and generate economically significant gains in volatility targeting exercise over multiple horizons. However, results show no evidence in favor of a unique duration‐augmented model. The predictive ability of price duration is supported by a number of robustness checks, including alternative estimation windows, bull and bear market states, and alternative thresholds that define price events.
Cortical plasticity in central vision loss: Cortical thickness and neurite structure
Authors: Defenderfer Matthew K. , Demirayak Pinar , Fleming Leland L. , DeCarlo Dawn K. , Stewart Paul , Visscher Kristina M. 
Published: May 2023

AbstractLate‐stage macular degeneration (MD) often causes retinal lesions depriving an individual of central vision, forcing them to learn to use peripheral vision for daily tasks. To compensate, many patients develop a preferred retinal locus (PRL), an area of peripheral vision used more often than equivalent regions of spared vision. Thus, associated portions of cortex experience increased use, while portions of cortex associated with the lesion are deprived of sensory input. Prior research has not well examined the degree to which structural plasticity depends on the amount of use across the visual field. Cortical thickness, neurite density, and orientation dispersion were measured at portions of cortex associated with the PRL, the retinal lesion, and a control region in participants with MD as well as age‐matched, gender‐matched, and education‐matched controls. MD participants had significantly thinner cortex in both the cortical representation of the PRL (cPRL) and the control region, compared with controls, but no significant differences in thickness, neurite density, or orientation dispersion were found between the cPRL and the control region as functions of disease or onset. This decrease in thickness is driven by a subset of early‐onset participants whose patterns of thickness, neurite density, and neurite orientation dispersion are distinct from matched control participants. These results suggest that people who develop MD earlier in adulthood may undergo more structural plasticity than those who develop it late in life.
Leveraging relational analytics in human resource research and practice
Authors: Soltis Scott M. , Methot Jessica R. , Gittell Jody Hoffer , Harris T. Brad 
Published: May 2023

AbstractRelational analytics—the leveraging of data on workplace relationships—acts as a complement to traditional people analytics and moves scholars and practitioners closer to an understanding of the realities of the modern workplace. This article serves as a primer for relational analytics by highlighting the potential of the perspective for human resources, the levels of analysis that can be used to study relational analytics, and some of the common theoretical perspectives employed in this area. We introduce the 15 articles in the special issue by positioning them within broader trends we see in the field of relational analytics. Finally, we provide commentary on challenges and future directions in relational analytics with a focus on how the articles in this special issue may serve as examples of best practices and inspiration for future research and practice.
Study on anaerobic fermentation of waste activated sludge to produce volatile fatty acids by thermal–rhamnolipid treatment
Authors: Chuanchuan Dou , Yuling Liu , Penghe Zhao , Shuaishuai Li , Yang Jia 
Published: May 2023

AbstractBACKGROUNDThe extracellular polymer and cell wall of waste activated sludge serve as chemical barriers for anaerobic fermentation, limiting the recycling and utilization of organic carbon contained in the sludge. To improve the efficiency of anaerobic fermentation, this paper proposes thermal–rhamnolipid (T‐RL) pretreatment technology and systematically discusses the effect of T‐RL treatment on the anaerobic fermentation of waste activated sludge for acid production from the perspective of organic matter dissolution characteristics, total volatile fatty acid (VFA) content, composition and microbial changes.RESULTST‐RL treatment significantly promoted the dissolution of organic matter in sludge, with soluble chemical oxygen demand solubilizing to 6097.78–7375.56 mg L−1, soluble carbohydrates solubilizing to 207.38–274.74 mg L−1, and the dissolution of soluble proteins and DNA also at a high level. Both treatment factors significantly promoted the enrichment of acid‐producing microorganisms, especially acetic acid‐producing microorganisms. The best VFA production conditions of T‐RL treatment were 60 mg g−1 total solids and 70 °C, and the yield was 3294.96 mg L−1. Compared with thermal and RL treatments, the optimum yield of T‐RL treatment increased by 33.16% and 22.06%, respectively. The VFA components in the T‐RL group were mainly acetic acid and propionic acid. Under optimal conditions, the proportion of acetic acid and propionic acid reached 77%.CONCLUSIONT‐RL treatment can promote sludge disintegration and organic matter conversion through the coupling effect of thermal and RL treatments, providing sufficient and high‐quality substrate for acid‐producing microorganisms, effectively enriching VFA producers, and thus improving the efficiency of anaerobic fermentation for VFA production. This treatment technology will provide some beneficial references for the future utilization of sludge resources. © 2023 Society of Chemical Industry (SCI).
Assessment of circulating extracellular vesicles from calorie‐restricted mice and humans in ischaemic injury models
Authors: Jaimes Manuel S. V. , Liao Chia‐Te , Chen Max M. , Czosseck Andreas , Lee Tsung‐Lin , Chou Yu‐Hsiang , Chen Yung‐Ming , Lin Shuei‐Liong , Lai James J. , Lundy David J. 
Published: May 2023

AbstractCalorie restriction (CR) and fasting affect lifespan, disease susceptibility and response to acute injury across multiple animal models, including ischaemic injuries such as myocardial infarction or kidney hypoxia. The cargo and function of circulating extracellular vesicles (EV) respond to changes in host physiology, including exercise, injury, and other interventions. Thus, we hypothesised that EVs induced following CR may reflect some of the beneficial properties of CR itself. In a pilot study, EVs were isolated from mice following 21 days of 30 % CR, and from eight human donors after 72 h water‐only fasting. EV size, concentration and morphology were profiled by NTA, western blot and cryoEM, and their function was assessed using multiple assays related to ischaemic diseases. We found that EVs from post‐fasting samples better protected cardiac cells from hypoxia/reperfusion (H/R) injury compared to pre‐fasting EVs. However, there was no difference when used to treat H/R‐injured kidney epithelial cells. Post‐fasting derived EVs slowed the rate of fibroblast migration and slightly reduced macrophage inflammatory gene expression compared to pre‐fasting derived EVs. Lastly, we compared miRNA cargos of pre‐ and post‐fasting human serum EVs and found significant changes in a small number of miRNAs. We conclude that fasting appears to influence EV cargo and function, with varied effects worthy of further exploration.
Muscle‐specific, liver‐detargeted adeno‐associated virus gene therapy rescues Pompe phenotype in adult and neonate Gaa−/− mice
Authors: Sellier P. , Vidal P. , Bertin B. , Gicquel E. , Bertil‐Froidevaux E. , Georger C. , van Wittenberghe L. , Miranda A. , Daniele N. , Richard I. , Gross D. A. , Mingozzi F. , Collaud F. , Ronzitti G. 
Published: May 2023

AbstractPompe disease (PD) is a neuromuscular disorder caused by acid α‐glucosidase (GAA) deficiency. Reduced GAA activity leads to pathological glycogen accumulation in cardiac and skeletal muscles responsible for severe heart impairment, respiratory defects, and muscle weakness. Enzyme replacement therapy with recombinant human GAA (rhGAA) is the standard‐of‐care treatment for PD, however, its efficacy is limited due to poor uptake in muscle and the development of an immune response. Multiple clinical trials are ongoing in PD with adeno‐associated virus (AAV) vectors based on liver‐ and muscle‐targeting. Current gene therapy approaches are limited by liver proliferation, poor muscle targeting, and the potential immune response to the hGAA transgene. To generate a treatment tailored to infantile‐onset PD, we took advantage of a novel AAV capsid able to increase skeletal muscle targeting compared to AAV9 while reducing liver overload. When combined with a liver‐muscle tandem promoter (LiMP), and despite the extensive liver‐detargeting, this vector had a limited immune response to the hGAA transgene. This combination of capsid and promoter with improved muscle expression and specificity allowed for glycogen clearance in cardiac and skeletal muscles of Gaa−/− adult mice. In neonate Gaa−/−, complete rescue of glycogen content and muscle strength was observed 6 months after AAV vector injection. Our work highlights the importance of residual liver expression to control the immune response toward a potentially immunogenic transgene expressed in muscle. In conclusion, the demonstration of the efficacy of a muscle‐specific AAV capsid‐promoter combination for the full rescue of PD manifestation in both neonate and adult Gaa−/− provides a potential therapeutic avenue for the infantile‐onset form of this devastating disease.
Changes in, and correlation analysis of, volatile compounds, key enzymes, and fatty acids in lemon juice vesicles during freeze drying and hot‐air drying
Authors: Hu Jiaqi , Sun Xiyun , Yang Feifei , Vidyarthi Sriram K. , Xiao Hongwei , Liu Chunju , Duan Xiaojie , Wang Haiou 
Published: May 2023

AbstractBACKGROUNDLemon juice vesicles are distinguished by their unique and abundant volatile flavor compounds, which can undergo complex changes during drying. In this study, integrated freeze drying (IFD), conventional freeze drying (CFD), and hot‐air drying (AD) were used to dry lemon juice vesicles to investigate the changes in, and correlations among volatile compounds, fatty acids, and key enzyme activity during the drying process.RESULTSTwenty‐two volatile compounds were detected during the drying processes. Compared with fresh samples, seven compounds were lost in the dried samples after IFD, seven after CFS, and six after AD, and the loss rates of the total content of volatile compounds in the dried samples were 82.73% in CFD, more than 71.22% in IFD, and more than 28.78% in AD. In total, 1.015 mg/g of seven fatty acids were detected in the fresh samples; the content loss rates of total fatty acids after drying were 67.68% in AD, more than 53.00% in CFD, and more than 36.95% in IFD, respectively. During the three drying processes, IFD retained relatively higher enzyme activity in the samples.CONCLUSIONMany positive and negative correlations (P < 0.05) were observed among the key enzyme effects, fatty acids, and volatile compounds, showing close associations. The current work provides information that is important for the selection of suitable drying techniques for lemon juice vesicles and suggests how to control their flavor during the drying process. © 2023 Society of Chemical Industry.
Postharvest UV‐B exposure drives changes in primary metabolism, phenolic concentration, and volatilome profile in berries of different grape (Vitis vinifera L.) varieties
Authors: Narra Federica , Castagna Antonella , Palai Giacomo , Havlík Jaroslav , Bergo Anna Mascellani , D'Onofrio Claudio , Ranieri Annamaria , Santin Marco 
Published: May 2023

AbstractBACKGROUNDThe ultraviolet‐B (UV‐B) radiation can alter grape metabolism during berry development, but little is known on the effect of postharvest UV‐B exposure. In this study, we evaluated the effect of postharvest UV‐B exposure on berry primary and secondary metabolites in four grapevine varieties (Aleatico, Moscato bianco, Sangiovese, and Vermentino) in order to evaluate the possibility to increase the grape quality and its nutraceutical properties.RESULTSThe treatment did not significantly affect the berry primary metabolism in terms of organic acids, carbohydrates, and amino acids profile, regardless of the variety. UV‐B exposure reduced the total anthocyanin content, particularly the tri‐substituted and di‐substituted forms in Aleatico and Sangiovese, respectively. An overall negative effect of UV‐B irradiation on the flavonols profile of Aleatico, Moscato bianco, and Vermentino berries was found, whereas it enhanced the quercetin, myricetin and kaempferol concentration in Sangiovese. The free fraction of berry volatile organic compounds increased in UV‐B‐treated Aleatico and Moscato bianco berries, especially C13‐norisoprenoids and volatile phenols, as well as key monoterpenes, such as the linalool derivatives. However, higher concentrations of glycosylated monoterpenes and C13‐norisoprenoids were measured in Sangiovese and Vermentino berries treated with UV‐B.CONCLUSIONThis study provides new insights on the effect of postharvest UV‐B radiation on berry secondary metabolism, highlighting a different modulation between varieties and suggesting the potential use of this technique to increase some nutraceutical and quality characteristics of grape berry. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.