bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of two unilateral laryngoplasty techniques and their effect on arytenoid cartilage abduction in cats
Authors: Bonanno Giuseppe , White Robert N 
Published: May 2023

Objectives The aim of this study was to evaluate two unilateral laryngoplasty (arytenoid lateralisation) techniques for use in the surgical management of laryngeal paralysis in cats. Methods Left cricoarytenoid abduction (lateralisation) was performed in 20 ex vivo cat larynges; 10 following complete cricoarytenoid disarticulation (group LAA-dis) and 10 following no cricoarytenoid disarticulation (group LAA-nodis). For both groups, left arytenoid abduction (LAA) was measured in the resting and postoperative larynges using image analysis software. Measurements were evaluated using the Mann–Whitney U-test. For both groups, dorsal images of the postoperative larynges were assessed visually for evidence of epiglottic coverage of the entrance to the larynx. Results The mean percentage increase in LAA was 311.5% and 199.4% ( P <0.006) for group LAA-dis (complete cricoarytenoid disarticulation) and group LAA-nodis (no cricoarytenoid disarticulation), respectively. In both groups, there was no evidence of a lack of epiglottic coverage of the entrance of the larynx for any of the postoperative larynges. Conclusions and relevance Placement of a single, tensioned suture between the muscular process of the left arytenoid cartilage and the caudolateral aspect of the ipsilateral cricoid cartilage (unilateral cricoarytenoid lateralisation) resulted in abduction of the left arytenoid cartilage and a corresponding increase in the area of the rima glottidis on the operated side. The clinical significance of the difference in outcome between left cricoarytenoid abduction following complete cricoarytenoid disarticulation and abduction following no cricoarytenoid disarticulation remains unclear, and both might be considered appropriate for the surgical management of laryngeal paralysis in the cat.
Effectiveness of Waterpik® for oral hygiene maintenance in orthodontic fixed appliance patients: A randomised controlled trial
Authors: Tyler Daniel , Kang Jing , Goh Hock Hoe 
Published: May 2023

Objective: To establish whether the use of a WaterPik® alongside a manual toothbrush (WaterPik® + MTB) is more effective for maintaining oral hygiene compared to the use of a manual toothbrush alone (MTB) in patients wearing fixed orthodontic appliances. Design: A single-centre, two-arm, parallel-group, single-blind, randomised controlled clinical trial with a 1:1 allocation ratio. Setting: Orthodontic department at York Hospital, York Teaching Hospitals NHS Foundation Trust, UK. Participants: A total of 40 fit and well participants, aged 10–20 years, being treated with upper and lower fixed orthodontic appliances. Methods: Participants were randomly allocated, using stratified block randomisation, to the control group (MTB) or intervention group ‘(Waterpik® + MTB)’. Plaque, gingival and interdental bleeding indices were recorded at baseline, 8 weeks, 32 weeks and 56 weeks. A generalised linear mixed model was used to assess differences between groups. Results: An interim analysis of results was performed with 40 patients recruited and 85% of data collected. The overall mean differences between the groups were as follows: plaque index = 0.199 ( P = 0.88, 95% confidence interval [CI] −0.24 to 0.27); gingival index = −0.008 ( P = 0.94, 95% CI −0.22 to 0.20); and interdental bleeding index = 5.60 ( P = 0.563, 95% CI −13.22 to 24.42). No statistical difference between the two groups was found for any variable. The trial was stopped at this point. Conclusions: In terms of oral hygiene, our study did not find evidence to support the claim of benefit of using a Waterpik® in addition to a manual toothbrush for patients wearing fixed orthodontic appliances.
Chemical Constituents and Neuroprotective Activity of Hypericum hookerianum
Authors: Hong Vu Duy , Vu Van Tuan , Selvaraj Baskar , Tuyen Nguyen Manh , Khoi Nguyen Minh , Thuan Nguyen Duy , Thuong Phuong Thien , Lee Jae Wook 
Published: May 2023

Objective/Background The Hypericum hookerianum is widely distributed in Asian countries and prevalently used in traditional herbal medicines for its significant antioxidant, antipyretic, and anticancer properties. To date, very limited reports are available regarding the phytochemical profile, and there are no reports that have studied the neuroprotective potentials of this plant. Experimental Methods The aerial parts of H. hookerianum were collected from the Sapa mountainous district, Lao Cai province of Vietnam. The extracts were prepared using methanol, n-butanol, ethyl acetate, and chloroform and fractionated by normal-phase silica gel column chromatography to isolate compounds. The purity of the isolated compounds was analyzed by liquid chromatography combined with mass spectrometry. The structures were studied using nuclear magnetic resonance spectroscopy. All the isolates were evaluated for their neuroprotective activity against the neurotoxicity induced by glutamate in HT-22 hippocampal cells and 6-hydroxydopamine (6-OHDA) in SH-SY5Y neuroblastoma cells. The fluorescent dye calcein-AM image assay was used to measure the cell viability. Results Bioassay-guided fractionation of the aerial parts of H. hookerianum led to the isolation of 20 previously reported compounds (1-20), including 3 flavonoids (1-3), 2 xanthones (4 and 5), 1 chalcone (6), 4 lignans (7-0), 1 chromone-C-glycoside (11), 1 cinnamic acid derivative (12), 7 phloroglucinols (13-9), and 1 stilbene (20). The structures of the compounds were determined by spectroscopic methods and compared with the reported data. The extracts and isolated compounds showed neuroprotection in both cell models. The compounds 4-hydroxy-2,6,4′-trimethoxydihydrochalcone (6, EC50 = 1.48 µM) and sesamin (10, EC50 = 2.85 µM) exhibited significant neuroprotective effects in HT-22 and SH-SY5Y cells, respectively. Conclusion This is the first report on the neuroprotection of extracts and isolated compounds from H. hookerianum. The results provide information for further studies to develop products for the prevention and treatment of neurological disorders.
Comparison of Strength and Power Characteristics Before ACL Rupture and at the End of Rehabilitation Before Return to Sport in Professional Soccer Players
Authors: Maestroni Luca , Turner Anthony , Papadopoulos Konstantinos , Cohen Daniel , Sideris Vasileios , Graham-Smith Philip , Read Paul 
Published: May 2023

Background: Strength and power is often reduced on the involved versus contralateral limb and healthy controls after anterior cruciate ligament (ACL) reconstruction, but no study has compared with preinjury values at the time of return to sport (RTS). Hypothesis: Divergent recovery patterns in strength and power characteristics will be present at RTS relative to preinjury baseline data and healthy matched controls. Study Design: Cohort study. Level of Evidence: Level 3. Methods: Isokinetic strength tests, bilateral and single-leg countermovement jumps (CMJ; SLCMJ) were measured before ACL rupture in 20 professional soccer players. These then had surgical reconstruction (ACL group) and completed follow-up testing before RTS. Healthy controls (uninjured group) were tested at the same time as the ACL group preinjury. Values recorded at RTS of the ACL group were compared with preinjury. We also compared the uninjured and ACL groups at baseline and RTS. Results: Compared with preinjury, ACL normalized quadriceps peak torque of the involved limb (difference = -7%), SLCMJ height (difference = -12.08%), and Reactive Strength Index modified (RSImod) (difference = -5.04%) were reduced after ACL reconstruction. No significant reductions in CMJ height, RSImod, and relative peak power were indicated at RTS in the ACL group when compared with preinjury values, but deficits were present relative to controls. The uninvolved limb improved quadriceps (difference = 9.34%) and hamstring strength (difference = 7.36%) from preinjury to RTS. No significant differences from baseline were shown in SLCMJ height, power, and reactive strength of the uninvolved limb after ACL reconstruction. Conclusion: Strength and power in professional soccer players at RTS after ACL reconstruction were often reduced compared with preinjury values and matched healthy controls. Clinical Relevance: Deficits were more apparent in the SLCMJ, suggesting that dynamic and multijoint unilateral force production is an important component of rehabilitation. Use of the uninvolved limb and normative data to determine recovery may not always be appropriate.
Assessment of functionalities and attitude toward telemedicine for patients with cardiovascular disease
Authors: Knaepen Lieselotte , Falter Maarten , Scherrenberg Martijn , Dendale Paul , Desteghe Lien , Heidbuchel Hein 
Published: May 2023

Introduction Many patients with cardiovascular diseases are only seen by a physician once or twice a year unless urgent symptoms. Recent years have shown an increase in digital technologies to follow patients remotely, that is, telemedicine. Telemedicine can be supportive for follow-up of patients at continuous risk. This study investigated patients’ attitude toward telemedicine, the defined features they consider important and future willingness to pay. Methods Cardiology patients with various types of prior telemedicine follow-up or who never had a telemonitoring follow-up were included. A new self-developed survey was implemented electronically and took 5–10 min to complete. Results In total, 231 patients (191 telemedicine [T] and 40 controls [C]), were included. Most participants owned a smartphone (84.8%) and only 2.2% of the total participants did not own any digital device. The most important feature of telemedicine cited in both groups was personalization (i.e., personalized health tips based on medical history, 89.6%; personalized feedback on entered health parameters 86.1%). The most important motivating factor for the use of telemedicine is recommendation by a physician (84.8%), while the reduction of in-person visits is a minor reason (24.7%). Only half of the participants (67.1%) would be willing to pay for telemedicine tools in the future. Conclusion Patients with cardiovascular disease have a positive attitude to telemedicine, especially when it allows for more personalized care, and when it is advocated by the physician. Participants expect that telemedicine becomes part of reimbursed care. This calls for interactive tools with proven efficacy and safety, while guarding unequal access to care.
Determinants of Antenatal Care Visits in Bangladesh: A Quantile Regression Analysis
Authors: Akter Mst. Bithi , Mahmud Anis , Karim Md. Rezaul 
Published: May 2023

Background Maternal and infant mortality is a major problem in a developing country like Bangladesh and these deaths are mostly related to incomplete antenatal care (ANC) visits. Adequate ANC visits for women are crucial in controlling maternal and infant mortality. Aims To investigate factors associated with ANC visits among women of reproductive age (15-49) in Bangladesh using the Bangladesh Demographic Health Survey 2017–2018 (BDHS) data. Methods This study included 5012 respondents, of whom 2414 women (48.2%) were complete ANC visits and 2598 women (51.8%) were incomplete ANC visits. Quantile regression was used for analysis indicating that the effects of different covariates functioned differently across the utilization of antenatal care visits. The results revealed the women's educational level, birth order number, sex of household head, and wealth index were highly significant on the lower, middle, and higher quantiles of the number of incomplete ANC visits. Besides, in the higher quantiles (for example, 75% quantile), the place of residence was highly significant. For division variables, Rajshahi, Rangpur, and Khulna were highly significant in lower and middle quantiles, while Dhaka, Khulna, Mymensingh, and Rajshahi were insignificant in higher quantiles. Conclusions This study observed that education, wealth index, birth order of children, and place of residence are associated with utilization of ANC visits and significantly influence maternal mortality. These determinations can help healthcare programmers and policymakers to take appropriate policies and programs for complete antennal care visits among pregnant women in Bangladesh. In order to increase the number of ANC visits among women, it is necessary to establish a mutually coordinated and trusting relationship between the government, non-governmental organizations and NGOs.
A Community-Based Cross-Sectional Study on Latrine Utilization and Associated Factors Among Rural Community of East Meskan District, Gurage Zone, Southern Ethiopia
Authors: Zewede Elsabet Negussie , Bekele Nardos Tilahun , Negussie Yohannes Mekuria , Getahun Mihiret Shawel , Gurara Abenet Menene 
Published: May 2023

Background Using sanitary facilities is proven to enhance health and halt the spread of fecal-to-oral disease. Despite efforts to improve the availability of latrine facilities in developing countries like Ethiopia, finding a village that is entirely free of open defecation remains difficult. To determine the need for intervention programs and promote regular latrine usage, local data is essential. Objectives This study aimed to assess latrine utilization and associated factors among households in East Meskan District, Southern Ethiopia. Methods A community-based cross-sectional study was conducted among 630 households from April 15 to May 30, 2022. A simple random sampling technique was used to select the study households. Data were collected using an interviewer-administered structured questionnaire and an observational checklist. The collected data were then entered into Epi-Info version 7.1 and analyzed using SPSS version 21. In binary logistic regression analysis, independent variables with a P-value < .25 were considered candidates for multiple logistic regression analysis. The association was expressed in odds ratio with a 95% confidence interval (CI), and significance was declared at P-value < .05 in the final model. Results The magnitude of latrine utilization was 73.3% (95% CI: 69.7, 76.8) in the study district. Husband being family head (adjusted odds ratio [AOR] = 12.9; 95% CI: 5.78 ,28.90), being female (AOR = 16.4; 95% CI: 6.52, 41.27), family size less than 5 (AOR = 24.2; 95% CI: 11.49, 51.09), absence of school children (AOR = 0.3; 95% CI: 0.13, 0.51), and greater than 2 years since latrine was constructed (AOR = 14; 95% CI: 7.18, 27.41) had a significant association with latrine utilization. Conclusion In this study, utilization of latrines was low compared to the national target plan. Family head, sex, family size, presence of school children, and length of years in which the latrine was constructed were factors associated with latrine utilization. Thus, regular supervision of early latrine construction and utilization in communities is essential.
Unilateral Severe Condylar Resorption Following Internal Fixation in Bilateral Mandibular Condylar Fracture
Authors: Sasaki Ryo , Sangu Miyamoto Noriko , Okamoto Toshihiro 
Published: May 2023

Study Design Case Report Objective: A severe condylar resorption following internal fixation of the mandibular condylar fracture is rarely reported. Methods A 35-year-old female caused bilateral condylar base fracture due to fall. She had no connective tissue autoimmune disease, no temporomandibular joint (TMJ) problems, and no history of the orthodontic treatment. Each fractures of condyles were fixed by 2 none-locking titanium miniplates on ideal lines of the osteosynthesis. Results: 6 months after surgery, although patient showed no malocclusion and no TMJ pain, panoramic X-ray showed left side condylar head resorption. Twelve months after surgery, bilateral plate removal was performed. Twenty-six months after internal fixation, panoramic X-ray showed bone formation at the left side of the condylar head. Magnetic resonance imaging of the TMJ showed left TMJ disc displacement without reduction and severe deformity of the left condylar head. Conclusions The condylar resorption might be caused by increasing functional loading due to new position of the condyle following osteosynthesis. Possible contributors for condylar resorption following osteosynthesis were: (1) internal fixation in bilateral condylar fracture, (2) disc displacement without reduction, and (3) female patient (hormonally-mediated theory, eg estrogen). However, the mechanize of condylar resorption was unclear and the further studies were necessary.
LSC17 score complements genetics and measurable residual disease in acute myeloid leukemia: an ALFA study
Authors: Vasseur Loïc , Fenwarth Laurène , Lambert Jérôme , de Botton Stéphane , Figeac Martin , Villenet Céline , Heiblig Maël , Dumas Pierre-Yves , Récher Christian , Berthon Céline , Lemasle Emilie , Lebon Delphine , Lambert Juliette , Terré Christine , Celli-Lebras Karine , Dombret Hervé , Preudhomme Claude , Cheok Meyling , Itzykson Raphael , Duployez Nicolas 
Published: May 2023

Abstract
Whether the LSC17 gene expression can improve risk stratification in the context of next generation sequencing–based risk stratification and measurable residual disease (MRD) in patients with intensively treated AML has not been explored. We analyzed LSC17 in 504 adult patients prospectively treated in the ALFA-0702 trial. RUNX1 or TP53 mutations were associated with higher LSC1 scores while CEBPA and NPM1 mutations were associated with lower scores. Patients with high LSC17 scores had a lower rate of complete response (CR) in a multivariable analysis (odds ratio, 0.41; P = .0007), accounting for European LeukemiaNet 2022 (ELN22), age, and white blood cell count (WBC). LSC17-high status was associated with shorter overall survival (OS) (3-year OS: 70.0% vs 52.7% in patients with LSC17-low status; P &lt; .0001). In a multivariable analysis considering ELN22, age, and WBC, patients with LSC17-high status had shorter disease-free survival (DFS) (hazard ratio [HR], 1.36; P = .048) than those with LSC17-low status. In 123 patients with NPM1-mutated AML in CR, LSC17-high status predicted poorer DFS (HR, 2.34; P = .01), independent of age, WBC, ELN22 risk, and NPM1-MRD. LSC-low status and negative NPM1-MRD identified a subset comprising 48% of patients with mutated NPM1 with a 3-year OS from CR of 93.1% compared with 60.7% in those with LSC17-high status and/or positive NPM1-MRD (P = .0001). Overall, LSC17 assessment refines genetic risk stratification in adult patients with AML treated intensively. Combined with MRD, LSC17 identifies a subset of patients with NPM1-mutated AML with excellent clinical outcome.
Empagliflozin improves renal ischemia–reperfusion injury by reducing inflammation and enhancing mitochondrial fusion through AMPK–OPA1 pathway promotion
Authors: Yang Wenbo , Li Xiaoli , He Liujie , Zhu Shuyang , Lai Shicong , Zhang Xiaopeng , Huang Zixiong , Yu Biyue , Cui Chunping , Wang Qiang 
Published: May 2023

Abstract
Background
Renal ischemia–reperfusion injury (IRI) is one reason for renal transplantation failure. Recent studies have shown that mitochondrial dynamics is closely related to IRI, and that inhibition or reversal of mitochondrial division protects organs against IRI. Optic atrophy protein 1 (OPA1), an important factor in mitochondrial fusion, has been shown to be upregulated by sodium-glucose cotransporter 2 inhibitor (SGLT2i). Also, the antiinflammatory effects of SGLT2i have been demonstrated in renal cells. Thus, we hypothesized that empagliflozin could prevent IRI through inhibiting mitochondrial division and reducing inflammation.

Methods
Using hematoxylin–eosin staining, enzyme linked immunosorbent assay (ELISA), flow cytometry, immunofluorescent staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining, real-time PCR, RNA-sequencing, and western blot, we analyzed renal tubular tissue from in vivo and in vitro experiments.

Results
Through animal experiments and sequencing analysis, we first confirmed the protection against IRI and the regulation of mitochondrial dynamics-related factors and inflammatory factors by empagliflozin pretreatment. Then, through hypoxia/reoxygenation (H/R) cellular experiments, we confirmed that empagliflozin could inhibit mitochondrial shortening and division and upregulate OPA1 in human renal tubular epithelial cell line (HK-2) cells. Subsequently, we knocked down OPA1, and mitochondrial division and shortening were observed, which could be alleviated by empagliflozin treatment. Combined with the previous results, we concluded that OPA1 downregulation leads to mitochondrial division and shortening, and empagliflozin can alleviate the condition by upregulating OPA1. We further explored the pathway through which empagliflozin functions. Related studies have shown the activation of AMPK pathway by empagliflozin and the close correlation between the AMPK pathway and OPA1. In our study, we blocked the AMPK pathway, and OPA1 upregulation by empagliflozin was not observed, thus demonstrating the dependence of empagliflozin on the AMPK pathway.

Conclusion
The results indicated that empagliflozin could prevent or alleviate renal IRI through antiinflammatory effects and the AMPK–OPA1 pathway. Ischemia–reperfusion injury is an inevitable challenge in organ transplantation. It is necessary to develop a new therapeutic strategy for IRI prevention in addition to refining the transplantation process. In this study, we confirmed the preventive and protective effects of empagliflozin in renal ischemia–reperfusion injury. Based on these findings, empagliflozin is promising to be a preventive agent for renal ischemia–reperfusion injury and can be applied for preemptive administration in kidney transplantation.