bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant diversity and useful plant species in Nam Dong Conservation Area, Thanh Hoa province, northern Vietnam
Authors: Nguyen Cuong Huu , Averyanov L , Egorov A , Chu Quan Ngoc 
Published: March 2023

Abstract
The detailed floristic inventory of Nam Dong Conservation Area was undertaken during 2015-2021. For the survey, 11 plots were established and studied. Herbarium specimens for almost all observed plant species were collected in the studied area. They were used for identifications and documentation of plants cited in in this report. The study pays special attention to vascular plants notable for their high number of endemic and rare species in limestone karst ecosystems typical for Nam Dong Conservation Area. The results of our preliminary botanical survey, indicate that Nam Dong Conservation Area supports a rich and very diverse flora. The survey reports 1312 vascular plant species in 637 genera belonging to 180 families. May of recorded species are rare and narrowly endemic plants. Among them 2 taxa new for science, and 1 represents new record for the flora of Vietnam. Aside from the high plant diversity, the Nam Dong forests contain many economically important plants, widely used by local communities. Such species groups include numerous timber trees, medicinal, ornamental and edible plants.
Green way to identify the mineral composition of Borjomi water by conductometric method
Authors: Gonchar D S , Nikulina S N , Fateeva N Y , Cherikanova E A , Vasyukov A E , Smirnova E S 
Published: March 2023

Abstract
A conductometric method for quantitative determination of the volume fraction of added distilled water to mineral water is proposed based on the analysis of the dependence of the specific electrical conductivity of solutions prepared on the basis of Borjomi mineral water on the degree of its dilution with distilled water. The technique is fully consistent with the principles of “green” chemistry, since the “waste” is mineral water diluted with distilled water.
Preprint Highlight: Constriction forces imposed by basement membranes regulate developmental cell migration
Authors: Rios Daniel 
Published: March 2023

Extracellular matrix (ECM) stiffness affects many processes of ECM-associated cells, such as migration and differentiation. Whether ECM mechanical properties also affect distant cell populations remains largely unexplored. This preprint studies migration of border cells within Drosophila ovarian follicles as a model system. Border cells migrate toward the center of the follicle by moving between nurse cells, which are surrounded by follicle cells that are themselves encased by the basement membrane ECM. Decreasing stiffness of the follicle ECM reduced cortical tension of follicle cells, and of nurse cells indirectly, accelerating border cell migration. Increasing follicle cell cortical tension slowed border cell migration. These results may inform how ECM stiffness affects cell migration within other complex tubular tissues, such as blood vessels. This preprint has been assigned the following badges: New Hypothesis, New Materials, Cross-Validation Read the preprint on bioRxiv ( Molina López et al., 2022 ): https://doi.org/10.1101/2022.12.19.520983 .
Examining the Long-term Association Between Neighborhood Socioeconomic Status and Obesity and Obesity-related Unhealthy Behaviors Among Children: Results From the Fragile Families and Child Wellbeing Study
Authors: Kim Yeonwoo , Liao Yue , Colabianchi Natalie 
Published: March 2023

Abstract

Background
Literature has focused on neighborhood environments and their possible impacts on obesity and obesity-related behaviors. However, few longitudinal studies have examined the effect of neighborhood socioeconomic status (nSES) on childhood obesity.

Purpose
Investigate the longitudinal association between nSES and obesity and obesity-related unhealthy behaviors.

Methods
We obtained data from the Fragile Families and Child Wellbeing Study (N = 2,072). The main exposure was nSES (measured using an index of five variables representing wealth, income, education, and occupation from the Decennial Census 2000) at ages 3, 5, and 9. The outcome was children’s body mass index z-score (BMIz) at ages 5, 9, and 15. Three measures of obesity-related behaviors (i.e., child- or caregiver-reported soda/snack food intake, fast-food intake, and sedentary behaviors) at ages 5, 9, and 15 were included as mediators and outcomes. Cross-lagged path analyses were conducted.

Results
Higher nSES at a previous wave was associated with consuming less soda/snack foods (βs = −0.15 to −0.11 [varying by ages], p &lt; .05) and fast-food intake (βs = −0.21 to −0.14 [varying by ages], p &lt; .01), and less frequent sedentary behaviors (βs = −0.14 to −0.06 [varying by ages], p &lt; .01), but not with BMIz (βs = −0.08 to 0.05 [varying by ages], p &gt; .05). Unhealthy behaviors did not mediate the nSES–BMIz association at alpha .05.

Conclusion
Health policies need to target low-socioeconomic neighborhoods to shape healthy lifestyles in children. To develop effective interventions, future research needs to examine comprehensive potential mediators like obesity-related parenting skills, home environments, and built and social environments on the risk of childhood obesity and obesity-related behaviors.
II—Forms of Agreement in Plato’sCrito
Authors: Warren James 
Published: March 2023

AbstractCrito thinks Socrates should agree to leave the prison and escape from Athens. Socrates is also determined that he and Crito should have a ‘common plan of action’ (koinē boulē: 49d3), but he wants Crito to share his preferred plan of remaining and submitting to the court’s sentence. Much of the drama of the Crito is generated by the interplay of these two old friends, both determined that they should come to an agreement, but differing radically in what they think the two of them should agree to do. I show how agreements of various kinds—including agreements about how to agree—play important roles in the dialogue and how Socrates’ commitment to a certain method for determining what to do underpins his own integrity. What’s more, attention to that theme helps to explain one of the most pressing questions for any interpretation of the Crito: Why does Socrates choose, at the end of the dialogue, to present to Crito a speech in the voice of the personified laws of Athens?
Efficacy and safety of lebrikizumab in moderate-to-severe atopic dermatitis: 52-week results of two randomized double-blinded placebo-controlled phase III trials
Authors: Blauvelt Andrew , Thyssen Jacob P , Guttman-Yassky Emma , Bieber Thomas , Serra-Baldrich Esther , Simpson Eric , Rosmarin David , Elmaraghy Hany , Meskimen Eric , Natalie Chitra R , Liu Zhuqing , Xu Chenjia , Pierce Evangeline , Morgan-Cox MaryAnn , Garcia Gil Esther , Silverberg Jonathan I 
Published: March 2023

Abstract

Background
Lebrikizumab is a novel, high-affinity monoclonal antibody that selectively binds to interleukin (IL)-13.

Objectives
To evaluate the efficacy and safety of lebrikizumab monotherapy in adolescent and adult patients with moderate-to-severe atopic dermatitis (AD) over 52 weeks of treatment in ADvocate1 (NCT04146363) and ADvocate2 (NCT04178967).

Methods
Patients who responded to lebrikizumab 250 mg every 2 weeks (Q2W) at the end of the 16-week induction period were re-randomized 2 : 2 : 1 to receive lebrikizumab Q2W, lebrikizumab 250 mg every 4 weeks (Q4W) or placebo Q2W (lebrikizumab withdrawal) for 36 additional weeks. Response at week 16 was defined as achieving a 75% reduction in Eczema Area Severity Index (EASI 75) or an Investigator’s Global Assessment (IGA) of 0 or 1, with a ≥ 2-point improvement and no rescue medication use. Multiple imputation was used to handle missing data. Intermittent use of topical therapy was permitted during the maintenance period.

Results
After 52 weeks, an IGA of 0 or 1 with a ≥ 2 point improvement was maintained by 71.2% of patients treated with lebrikizumab Q2W, 76.9% of patients treated with lebrikizumab Q4W and 47.9% of patients in the lebrikizumab withdrawal arm. EASI 75 was maintained by 78.4% of patients treated with lebrikizumab Q2W, 81.7% of patients treated with lebrikizumab Q4W and 66.4% of patients in the lebrikizumab withdrawal arm at week 52. Across treatment arms, proportions of patients using any rescue therapy were 14.0% (ADvocate1) and 16.4% (ADvocate2). During the combined induction and maintenance periods of ADvocate1 and ADvocate2, 63.0% of lebrikizumab-treated patients reported any treatment emergent adverse event, with most events (93.1%) being mild or moderate in severity.

Conclusions
After a 16-week induction period with lebrikizumab Q2W, lebrikizumab Q2W and Q4W maintained similar improvement of the signs and symptoms of moderate-to-severe AD, with a safety profile consistent with previously published data.
Efficacy and safety of baricitinib in combination with topical corticosteroids in paediatric patients with moderate-to-severe atopic dermatitis with an inadequate response to topical corticosteroids: results from a phase III, randomized, double-blind, placebo-controlled study (BREEZE-AD PEDS)
Authors: Torrelo Antonio , Rewerska Barbara , Galimberti Maria , Paller Amy , Yang Chin-Yi , Prakash Apurva , Zhu Danting , Pontes Filho Marco Antonio G , Wu Wen-Shuo , Eichenfield Lawrence F 
Published: March 2023

Abstract

Background
Baricitinib, an oral selective Janus kinase (JAK)1/JAK2 inhibitor, is approved in many countries for moderate-to-severe atopic dermatitis (AD) in adults who are candidates for systemic therapy.

Objectives
To evaluate the efficacy and safety of three doses of baricitinib in combination with low-to-moderate potency topical corticosteroids in paediatric patients with moderate-to-severe AD.

Methods
Patients (aged 2 to &lt; 18 years) were randomized (1 : 1 : 1 : 1) to once-daily baricitinib low dose (1 mg equivalent), medium dose (2 mg equivalent), high dose (4 mg equivalent) or placebo for 16 weeks. The primary endpoint was the proportion of patients achieving a validated Investigator Global Assessment® (vIGA-AD) of 0/1 with a ≥ 2-point improvement at week 16. Key secondary endpoints included the proportions of patients achieving ≥ 75% and ≥ 90% improvement in the Eczema Area and Severity Index (EASI-75 and EASI-90, respectively), ≥ 75% improvement in the SCORing Atopic Dermatitis (SCORAD 75), mean change from baseline in EASI score and proportion of patients achieving a 4-point improvement in the Itch Numeric Rating scale (NRS) for patients aged ≥ 10 years. Primary and key secondary efficacy analyses were conducted on the intent-to-treat population and adjusted for multiplicity. Safety analyses included all randomized patients who received ≥ 1 dose of study treatment.

Results
A total of 483 patients were randomized (mean age 12 years). The baricitinib 4 mg equivalent achieved a statistically significant (P &lt; 0.05) improvement vs. placebo on all 16-week endpoints (vIGA 0/1 with ≥ 2-point improvement, EASI-75, EASI-90, SCORAD 75, mean change in EASI score and Itch NRS 4-point improvement for patients aged ≥ 10 years). Improvement (P &lt; 0.05, non-multiplicity adjusted) was also observed for baricitinib 4 mg equivalent vs. placebo in the ability to fall asleep and in reduction of topical corticosteroid use. Few patients discontinued due to adverse events (1.6% for placebo and 0.6% for those treated with baricitinib). There were no deaths, venous thromboembolic events, arterial thrombotic events, major adverse cardiovascular events, malignancies, gastrointestinal perforations or opportunistic infections seen.

Conclusions
The results indicate that baricitinib offers a potential therapeutic option with a favourable benefit–risk profile for paediatric patients with moderate-to-severe AD who are candidates for systemic therapies.
Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies
Authors: Henriet Esther , Martin Elodie M , Jubin Pauline , Langui Dominique , Mannioui Abdelkrim , Stankoff Bruno , Lubetzki Catherine , Khakhalin Arseny , Zalc Bernard 
Published: March 2023

Abstract
In multiple sclerosis, while remarkable progress has been accomplished to control the inflammatory component of the disease, repair of demyelinated lesions is still an unmet need. Despite encouraging results generated in experimental models, several candidates favouring or promoting remyelination have not reached the expected outcomes in clinical trials. One possible reason for these failures is that, in most cases, during preclinical testing, efficacy was evaluated on histology only, while functional recovery had not been assessed.
We have generated a Xenopus laevis transgenic model Tg(mbp:GFP-NTR) of conditional demyelination in which spontaneous remyelination can be accelerated using candidate molecules. Xenopus laevis is a classic model for in vivo studies of myelination because tadpoles are translucent. We reasoned that demyelination should translate into loss of sensorimotor functions followed by behavioural recovery upon remyelination. To this end, we measured the swimming speed and distance travelled before and after demyelination and during the ongoing spontaneous remyelination and have developed a functional assay based on the visual avoidance of a virtual collision.
Here we show that alteration of these functional and clinical performances correlated well with the level of demyelination and that histological remyelination, assayed by counting in vivo the number of myelinating oligodendrocytes in the optic nerve, translated in clinical–functional recovery. This method was further validated in tadpoles treated with pro-remyelinating agents (clemastine, siponimod) showing that increased remyelination in the optic nerve was associated with functional improvement.
Our data illustrate the potential interest of correlating histopathological parameters and functional–clinical parameters to screen molecules promoting remyelination in a simple in vivo model of conditional demyelination.
A Broad Spectrum of Possibilities: Spectrum Scores as a Unifying Metric of Antibiotic Utilization
Authors: Ilges Dan , Tande Aaron J , Stevens Ryan W 
Published: March 2023

Abstract
Days of therapy (DOT) currently serve as the standard antimicrobial utilization metric. However, by assigning the same weight to each agent rather than accounting for differences in spectrum of activity, DOT ignore key differences between agents that are fundamental to infectious diseases and critical to antimicrobial stewardship. Spectrum scoring assigns numeric values to individual antibiotic agents to quantify their spectrum of activity, allowing for the normalization of antibiotic utilization data. When used in conjunction with traditional metrics, spectrum scores may offer further clarity to antibiotic utilization; however, issues related to development, application, and standardization of spectrum scores remain. Despite these challenges, the potential applications of spectrum scores are vast. Here, we summarize existing data and explore the future of spectrum scoring, including application to both data analysis and routine patient care, use in inpatient and outpatient settings, integration within the electronic medical record, and opportunities for future research.
Ochratoxin A degrading enzymes ofStenotrophomonassp. 043-1a
Authors: Gonaus Christoph , Wieland Laura , Thallinger Gerhard G , Prasad Shreenath 
Published: March 2023

AbstractOchratoxin A is a secondary metabolite that acts as a mycotoxin and is produced by Aspergillus, Penicillium, and other fungal species. It is a threat to animal and human health due to nephrotoxic, carcinogenic, and genotoxic properties and its widespread incidence in agricultural products. To reduce this threat, biological remediation processes are of growing interest. The aerobic gram-negative bacterium Stenotrophomonassp. 043-1a, isolated from soil, was previously shown to degrade ochratoxin A into the non-toxic ochratoxin α and l-phenylalanine (Schatzmayr et al. 2002). However, the enzyme or enzymes catalyzing this reaction in this strain remained elusive. Here, we report the targeted purification of Stenotrophomonassp. 043-1a lysate via ammonium sulfate precipitation, size-exclusion chromatography, and hydrophobic interaction chromatography to identify the ochratoxin A degrading enzymes by subsequent peptide fragment fingerprinting. The metallo-dependent hydrolase Chr1_3858681_3267 and a member of the peptidase S9 family, Chr1_3858681_771, were shown to degrade ochratoxin A. This was, to our knowledge, the first report of an ochratoxin A degrading enzyme from the peptidase S9 family.
Chemical Site Preparation Mixtures, Application Timing, and Herbaceous Weed Control Impacts on Loblolly Pine Development Through Six Years
Authors: Clabo David C , David Dickens E 
Published: March 2023

AbstractChemical site preparation is a critical management practice for southern pine plantation establishment. Imazapyr and ester triclopyr are frequently used for chemical site preparation in the Lower Coastal Plain to control waxy leaf shrub species, yet limited information is available on optimal imazapyr and triclopyr tank mixes and individual herbicide application timing(s) to achieve maximum loblolly pine (Pinus taeda L.) growth. The objectives of this study were to determine the survival, growth, and stem defect response of 6-year-old loblolly pine to four chemical site preparation treatments plus an untreated control, three application timings, and first year herbaceous weed control (hwc) on a poorly drained flatwoods site. Treatments included two imazapyr and triclopyr rates applied as tank mixes, triclopyr and imazapyr alone, and an untreated control with hwc applied to half of each treatment plot. Results showed no treatment had superior survival or growth, whereas September-applied imazapyr and triclopyr tank mixes tended to result in improved growth. Triclopyr alone along with first year, banded hwc resulted in similar growth to imazapyr and triclopyr tank mixes. First-year hwc significantly enhanced survival and growth. Stem defects were more common in the chemical site preparation treatments than the control.Study Implications: Age-6-year results from this study indicate that loblolly pine (Pinus taeda L.) growth following application of chemical site preparation tank mixes containing imazapyr and triclopyr applied during September or October tended to be superior to July applications. Triclopyr applied alone with first growing season herbaceous weed control (hwc) may offer satisfactory near- and long-term loblolly pine growth as imazapyr and triclopyr tank mixtures on sites with similar competing vegetation. These findings may allow for less herbicide use on sites previously managed for pine production and show the importance of first year hwc for Coastal Plain flatwoods sites.
Recombination hotspots in soybean [Glycine max (L.) Merr.]
Authors: McConaughy Samantha , Amundsen Keenan , Song Qijian , Pantalone Vince , Hyten David 
Published: March 2023

Abstract
Recombination allows for the exchange of genetic material between two parents, which plant breeders exploit to make improved cultivars. This recombination is not distributed evenly across the chromosome. Recombination mostly occurs in euchromatic regions of the genome and even then, recombination is focused into clusters of crossovers termed recombination hotspots. Understanding the distribution of these hotspots along with the sequence motifs associated with them may lead to methods that enable breeders to better exploit recombination in breeding. To map recombination hotspots and identify sequence motifs associated with hotspots in soybean [Glycine max (L.) Merr.], two biparental recombinant inbred lines populations were genotyped with the SoySNP50k Illumina Infinium assay. A total of 451 recombination hotspots were identified in the two populations. Despite being half-sib populations, only 18 hotspots were in common between the two populations. While pericentromeric regions did exhibit extreme suppression of recombination, 27% of the detected hotspots were located in the pericentromeric regions of the chromosomes. Two genomic motifs associated with hotspots are similar to human, dog, rice, wheat, drosophila, and arabidopsis. These motifs were a CCN repeat motif and a poly-A motif. Genomic regions spanning other hotspots were significantly enriched with the tourist family of mini-inverted-repeat transposable elements that resides in &lt;0.34% of the soybean genome. The characterization of recombination hotspots in these two large soybean biparental populations demonstrates that hotspots do occur throughout the soybean genome and are enriched for specific motifs, but their locations may not be conserved between different populations.
Contamination detection and microbiome exploration with GRIMER
Authors: Piro Vitor C , Renard Bernhard Y 
Published: March 2023

Abstract

Background
Contamination detection is a important step that should be carefully considered in early stages when designing and performing microbiome studies to avoid biased outcomes. Detecting and removing true contaminants is challenging, especially in low-biomass samples or in studies lacking proper controls. Interactive visualizations and analysis platforms are crucial to better guide this step, to help to identify and detect noisy patterns that could potentially be contamination. Additionally, external evidence, like aggregation of several contamination detection methods and the use of common contaminants reported in the literature, could help to discover and mitigate contamination.

Results
We propose GRIMER, a tool that performs automated analyses and generates a portable and interactive dashboard integrating annotation, taxonomy, and metadata. It unifies several sources of evidence to help detect contamination. GRIMER is independent of quantification methods and directly analyzes contingency tables to create an interactive and offline report. Reports can be created in seconds and are accessible for nonspecialists, providing an intuitive set of charts to explore data distribution among observations and samples and its connections with external sources. Further, we compiled and used an extensive list of possible external contaminant taxa and common contaminants with 210 genera and 627 species reported in 22 published articles.

Conclusion
GRIMER enables visual data exploration and analysis, supporting contamination detection in microbiome studies. The tool and data presented are open source and available at https://gitlab.com/dacs-hpi/grimer.