bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ranbp1modulates morphogenesis of the craniofacial midline in mouse models of 22q11.2 deletion syndrome
Authors: Paronett Elizabeth M , Bryan Corey A , Maynard Megan E , Goroff Julia A , Meechan Daniel W , LaMantia Anthony-Samuel , Maynard Thomas M 
Published: February 2023

AbstractFacial dysmorphology is a hallmark of 22q11.2 deletion syndrome (22q11DS). Nearly all affected individuals have facial features characteristic of the syndrome: a vertically long face with broad nasal bridge, narrow palpebral fissures and mild micrognathia, sometimes accompanied by facial skeletal and oropharyngeal anomalies. Despite the frequency of craniofacial dysmorphology due to 22q11.2 deletion, there is still incomplete understanding of the contribution of individual 22q11 genes to craniofacial and oropharyngeal development. We asked whether homozygous or heterozygous loss of function of single 22q11 genes compromises craniofacial and/or oropharyngeal morphogenesis related to these 22q11DS phenotypes. We found that Ranbp1, a 22q11DS gene that mediates nucleocytoplasmic protein trafficking, is a dosage-dependent modulator of craniofacial development. Ranbp1−/− embryos have variably penetrant facial phenotypes, including altered facial morphology and cleft palate. This 22q11DS-related dysmorphology is particularly evident in the midline of the facial skeleton, as evidenced by a robustly quantifiable dysmorphology of the vomer, an unpaired facial midline bone. 22q11DS-related oropharyngeal phenotypes reflect Ranbp1 function in both the cranial neural crest and cranial ectoderm based upon tissue-selective Ranbp1 deletion. Analyses of genetic interaction show that Ranbp1 mutation disrupts BMP signaling-dependent midline gene expression and BMP-mediated craniofacial and cranial skeletal morphogenesis. Finally, midline defects that parallel those in Ranbp1 mutant mice are observed at similar frequencies in the LgDel 22q112DS mouse model. Apparently, Ranbp1 is a modulator of craniofacial development, and in the context of broader 22q11 deletion, Ranbp1 mutant phenotypes mirror key aspects of 22q11DS midline facial anomalies.
Spectral Changes of EEG Following a 6-Week Low-Dose Oral Ketamine Treatment in Adults With Major Depressive Disorder and Chronic Suicidality
Authors: Anijärv T E , Can A T , Gallay C C , Forsyth G A , Dutton M , Mitchell J S , Hermens D F , Lagopoulos J 
Published: February 2023

AbstractBackgroundKetamine has considerable therapeutic potential in alleviating major depressive disorder and chronic suicidality. However, the clinical diagnosis of neuropsychiatric disorders requires more robust diagnostic criteria. Electroencephalography (EEG) has shown promise in classifying depressive and suicidal patients from healthy individuals. The present study aimed to identify changes in the spectral properties of EEG in patients with major depressive disorder and chronic suicidality after completing the 6-week Oral Ketamine Trial on Suicidality with follow-up occurring 4 weeks after final ketamine treatment and determine associations between EEG spectral output and clinical symptoms.MethodsParticipants (n = 25) had 4-minute eyes closed resting state EEG recorded at frontal, temporal, centro-parietal, and occipital regions. Spectral analysis was performed with Welch’s power spectrum density method, and the power of 4 distinct frequency bands was analyzed: theta, alpha, low-beta, and high-beta. Correlation analyses between changes in clinical symptoms and spectral power were conducted using Spearman’s ranked correlation.ResultsBetween pre- and posttreatment, only centro-parietal alpha power decreased. Between posttreatment and follow-up, centro-parietal alpha increased again in addition to increases in temporal alpha, centro-parietal and temporal theta, and occipital low-beta and decreases in occipital theta and temporal low-beta. Additionally, the decrease of occipital theta positively correlated with clinical subscales for depression and stress.ConclusionsEEG spectral analysis revealed significant changes in theta, alpha, and low-beta frequency bands. Alpha band showed initial changes after treatment; however, this trended back toward baseline levels after the treatment cessation. In contrast, theta and low-beta showed significant power changes only after the treatment had ended.
Human Papillomavirus Intermittence and Risk Factors Associated With First Detections and Redetections in the Ludwig-McGill Cohort Study of Adult Women
Authors: Malagón Talía , Trottier Helen , El-Zein Mariam , Villa Luisa L , Franco Eduardo L ,   Ludwig-McGill Cohort Study, Paulo Sao , Baggio Maria Luiza , Galan Lenice , Sobrinho João Simão , Mann Prado José Carlos , Termini Lara , Costa Maria Cecília , Miyamura Romulo , Trevisan Andrea , Thomann Patricia , Candeias João , Sichero Laura , Rahal Paula , Ruiz Antonio , Kaiano Jane , Santos Monica , Savio Patricia , Maciag Paulo , Rabachini Tatiana , Ferreira Silvaneide , Villa Luisa , El-Zein Mariam , Rousseau Marie-Claude , Mahmud Salaheddin , Schlecht Nicolas , Trottier Helen , Richardson Harriet , Ferenczy Alex , Rohan Thomas , Chevarie-Davis Myriam , Louvanto Karolina , Tota Joseph , Shaw Eileen , Ramanakumar Agnihotram , Duarte Eliane , Kulaga Sophie , Robitaille Juliette , Franco Eduardo 
Published: February 2023

Abstract

Background
We assessed the incidence and risk factors for first detection and redetection with the same human papillomavirus (HPV) genotype, and prevalence of cytological lesions during HPV redetections.

Methods
The Ludwig-McGill cohort study followed women aged 18–60 years from São Paulo, Brazil in 1993–1997 for up to 10 years. Women provided cervical samples for cytology testing and HPV DNA testing at each visit. A redetection was defined as a recurring genotype-specific HPV positive result after 1 or more intervening negative visits. Predictors of genotype-specific redetection were assessed using adjusted hazard ratios (aHR) with Cox regression modeling.

Results
In total, 2184 women contributed 2368 incident HPV genotype-specific first detections and 308 genotype-specific redetections over a median follow-up of 6.5 years. The cumulative incidence of redetection with the same genotype was 6.6% at 1 year and 14.8% at 5 years after the loss of positivity of the first detection. Neither age (aHR 0.90; 95% confidence interval [CI], .54–1.47 for ≥45 years vs &lt; 25 years) nor new sexual partner acquisition (aHR 0.98; 95% CI, .70–1.35) were statistically associated with genotype-specific redetection. High-grade squamous intraepithelial lesion prevalence was similar during first HPV detections (2.9%) and redetection (3.2%).

Conclusions
Our findings suggest many HPV redetections were likely reactivations of latent recurring infections.
Investigation of admission serum creatinine as a predictor of hospital length of stay in triple-vaccinated COVID-19 inpatients
Authors: Rouka Erasmia , Livanou Eleni , Sinis Sotirios , Dimeas Ilias , Pantazopoulos Ioannis , Papagiannis Dimitrios , Malli Foteini , Kotsiou Ourania , Gourgoulianis Konstantinos I 
Published: February 2023

Abstract

Background
This study assessed the association between admission kidney function and the duration of hospitalization in triple-vaccinated coronavirus disease 2019 (COVID-19) inpatients during the omicron surge in Larissa, central Greece.

Methods
Regression analysis was used to estimate the effect of kidney function biomarkers on hospital length of stay (LoS) within a dataset from a cohort of 51 subjects.

Results
Sex- and age-adjusted admission serum creatinine was associated with hospital LoS (p=0.034).

Conclusions
Serum creatinine concentration on admission should be further evaluated as a possible clinical predictor of hospital LoS among triple-vaccinated COVID-19 inpatients both at the country and global level.
A carbapenem-focused antimicrobial stewardship programme implemented during the COVID-19 pandemic in a setting of high endemicity for multidrug-resistant Gram-negative bacteria
Authors: Spernovasilis Nikolaos , Kritsotakis Evangelos I , Mathioudaki Anna , Vouidaski Alexandra , Spanias Christos , Petrodaskalaki Maria , Ioannou Petros , Chamilos Georgios , Kofteridis Diamantis P 
Published: February 2023

Background

Background
Greece is among the countries characterized by high rates of antimicrobial resistance and high consumption of antibiotics, including carbapenems.

Objectives
To measure the impact of a carbapenem-focused antimicrobial stewardship programme (ASP) on the antibiotic consumption and patient outcomes in a Greek tertiary hospital during the COVID-19 pandemic.

Methods
A quasi-experimental, before–after study, comparing a 12 month pre-intervention period with a 12 month intervention period in which a carbapenem-focused ASP was implemented.

Results
A total of 1268 patients were enrolled. The proportion of admitted patients who received carbapenems decreased from 4.1% (842 of 20 629) to 2.3% (426 of 18 245) (−1.8%; P &lt; 0.001). A decrease of −4.9 DDD/100 patient-days (PD) (95% CI −7.3 to −2.6; P = 0.007) in carbapenem use and an increase in the use of piperacillin/tazobactam [+2.1 DDD/100 PD (95% CI 1.0–3.3; P = 0.010)] were observed. Thirty-day mortality following initiation of carbapenem treatment and all-cause in-hospital mortality remained unaltered after ASP implementation. In contrast, length of hospital stay increased (median 17.0 versus 19.0 days; P &lt; 0.001), while the risk of infection-related readmission within 30 days of hospital discharge decreased (24.6% versus 16.8%; P = 0.007). In the post-implementation period, acceptance of the ASP intervention was associated with lower daily hazard of in-hospital death [cause-specific HR (csHR) 0.49; 95% CI 0.30–0.80], lower odds of 30 day mortality (OR 0.36; 95% CI 0.18–0.70) and higher rate of treatment success (csHR 2.45; 95% CI 1.59–3.77).

Conclusions
Implementing and maintaining a carbapenem-focused ASP is feasible, effective and safe in settings with high rates of antimicrobial resistance, even during the COVID-19 pandemic.
The Stability of Synthetic Cathinones and the Study of Potential Intake Biomarkers in the Biological Material from a Case of 3-CMC Poisoning
Authors: Romańczuk Agnieszka , Rojek Sebastian , Synowiec Kamil , Maciów-Głąb Martyna , Kula Karol , Rzepecka-Woźniak Ewa 
Published: February 2023

Abstract
The objective of the present study was to identify the metabolites of synthetic cathinone (SC), 3-chloromethcathinone (3-CMC), and to select a potential intake biomarker for this compound. The basis of the experiment was the analysis of blood and urine samples from a case of fatal poisoning with this substance. We also evaluated the stability of 3-CMC and the selected potential biomarker, the dihydro-3-CMC metabolite, depending on the time elapsed since the autopsy as well as the storage conditions of the biological material. Studies have shown that 3-CMC shows low stability in the biological material. However, acidification of the biological material or storage at low temperatures has a great impact on maintaining its stability. It is crucial to monitor the presence of the dihydro-3-CMC metabolite in the biological material during analysis, which showed high stability under all of the given storage conditions. SCs might not be detected in the biological material and, if they are, the determined concentrations at the time of testing may not correspond to the actual concentrations at the time of the event or death. Consequently, the interpretation of the results obtained for 3-CMC and dihydro-3-CMC in terms of assessing their toxicity and possible cause of death is difficult. The area of research into the search for other intake biomarkers of unstable halogen derivatives of SCs remains open.
Geographic Variation in Survival and Growth of Atlantic White-Cedar (Chamaecyparis Thyoides [L.] B.S.P.): Implications for Artificial Regeneration of a Declining Species
Authors: Olson Matthew , Lubas Andrew , Mylecraine Kristin 
Published: February 2023

Abstract
Atlantic white-cedar (Chamaecyparis thyoides [L.] B.S.P.; AWC), a wetland tree species native to the eastern United States, is declining rangewide. This decline has stimulated interest in restoring AWC in parts of its range. However, restoration could benefit from more research on this species, especially that focus on where to source seedlings for artificial reforestation. This study presents results out to years 18–20 from a southern New Jersey common garden established in the early 2000s as part of the first rangewide provenance planting of AWC. The goal of this research was to expand our understanding of intraspecific variation in AWC linked to the geographic origin of genetic sources. Results indicated that genotype performance was linked to their geographic origin and that local sources were among the top performers when taking all metrics into consideration (e.g., survival, height, basal area). Top-performing provenances were sourced within plus or minus two decimal degrees latitude of the planting site. Although local sources remain suitable for artificial regeneration of this species in southern New Jersey, nearby southern sources could be deployed to achieve out-planting success and conservation of this imperiled species in a warming climate. We hope this work will inform AWC restoration efforts and bring visibility to this declining wetland species.
Imperatorin derivative OW1, a new vasoactive compound, attenuates cell proliferation and migration by inhibiting Nox1-mediated oxidative stress
Authors: Zhou Nan , Yong Suyun , Shi Xianpeng , Zhang Peng , Wang Jianhua 
Published: February 2023

Abstract

Objectives
Reactive oxygen species (ROS) are involved in the structural remodelling of vascular segments and vascular beds. We identified a new imperatorin derivative, OW1, which has significant effects on vasodilation and inhibits vascular remodelling in hypertensive rats. In this study, we investigated whether OW1 inhibits vascular cell proliferation and migration by attenuating Nox1-ROS signalling.

Methods
Vascular smooth muscle cells (VSMCs) were treated with OW1 (1, 3 and 10 µmol/L) for 24 h incubation, and it has been analysed for proliferation and peroxidation levels. Moreover, the mRNA and protein levels of nicotinamide adenine dinucleotide phosphate oxidase (Noxs) were measured by RT-PCR and western blot. Furthermore, Nox1-ROS-MAPK/MMP mediated cell proliferation was detected by western blot.

Key findings
Ang II-induced increases in the levels of peroxidation and Noxs in VSMCs were also inhibited by OW1. OW1 attenuates cell proliferation and migration through the MAPK pathway and MMPs. OW1 treatment had no significant effects on cell migration, ROS levels, or the expression of phosphorylated MAPKs in VSMCs when Nox1 was knocked down. OW1 reduced ROS levels and expression of phosphorylated MAPKs in NIH3T3 cells with a Nox1 overexpression plasmid.

Conclusion
OW1 may inhibit vascular remodelling by downregulating the Nox1-ROS-MAPK/MMP signalling pathway.
Cutibacterium acnesin breast implants: an underestimated bacterial infection and review of the literature
Authors: Hanna Sam , Manuel Shauna , Baker Jenalle , Diab Jason , Clement Zackariah 
Published: February 2023

AbstractThe role of bacteria and breast implant illness is an emerging area of interest for surgeons and clinicians. The most common cause of surgical readmission remains post-operative infectious complications. Cutibacterium acnes is an anaerobic, gram-positive organism that is part of the normal human microbiota. In certain circumstances, it may cause chronic infections and capsular contractures in breast implant-related complications. This case series outlines patients with bilateral capsular contractures and growth of C. acnes. The patients were managed surgically with the removal of bilateral breast implants with en bloc capsulectomy and oral antibiotics without complications. This report will outline the pathology of C. acnes, association with breast implant-associated anaplastic large cell lymphoma and review of the literature.
Sexual well-being in patients with early-stage breast cancer at 1- and 2-year follow-up
Authors: Huberts Anouk S , Clarijs Marloes E , Pastoor Hester , van Rosmalen Mandy , Koppert Linetta B 
Published: February 2023

AbstractBackgroundSexual well-being (SWB) is an important aspect of overall quality of life and should therefore be considered when measuring the effect of breast cancer on daily life.AimTo identify positive and negative predictive factors associated with change in SWB 1 year after diagnosis (T12; hereafter, ∆SWB) and whether SWB changes the year after.MethodsAll data were derived from an online patient-reported outcome measure that included patients aged &gt;18 years who were treated for breast cancer between October 2015 and March 2022 at the Erasmus University Medical Center. Multivariable linear regression was used to analyze the association between demographic- and disease-specific variables and change in SWB between time of diagnoses (T0) and one year after (T12) (∆SWB). For defining the clinical relevance of ∆SWB, patients were divided into 3 groups based on their SWB score at T12: decreased, stable, and improved. Wilcoxon signed rank test was used to test the difference in SWB between T12 and T24 (2 years after diagnosis) in all 3 groups.OutcomesOutcomes included the associations between demographic- and disease-specific variables and ∆SWB (T0 vs T12) and change in SWB the year after (T12 vs T24).ResultsAn overall 204 patients were included, with a mean age of 51.7 years (SD, 12.8) and a mean SWB score of 64.3 (SD, 20.9) at T0. Body mass index &gt;30 kg/m2 at T0 had a significant negative association (β = −8.369, P = .019) with ∆SWB. Reconstruction (β = 20.136, P &lt; .001) and mastectomy (β = 11.157, P &lt; .001) had a significant positive association with ∆SWB vs lumpectomy. Change in psychological well-being had a significant positive relation to ∆SWB (β = 0.349, P &lt; .001). Patients with decreased SWB at T12 did not improve the year after (P = .376).Clinical ImplicationsBy identifying the variables that are associated with decreased SWB during the trajectory of breast cancer treatment and by defining the clinical relevance of decreased SWB, patient groups can be targeted and offered extra support.Strengths and LimitationsThis study is one of the first to analyze the development of SWB, instead of sexual function, over time in patients with breast cancer, and it uses data over a longer period. However, only one-third of the patients responded to the SWB domains at both time points.ConclusionType of operation, body mass index &gt;30, and change in psychological well-being were associated with ∆SWB. Patients with decreased SWB 1 year after diagnosis tended not to improve or normalize the year after, indicating that intervention is needed to restore SWB in this specific group.
The woody plant-degrading pathogen Lasiodiplodia theobromae effector LtCre1 targets the grapevine sugar-signaling protein VvRHIP1 to suppress host immunity
Authors: Xing Qikai , Zhou Xiangui , Cao Yang , Peng Junbo , Zhang Wei , Wang Xuncheng , Wu Jiahong , Li Xinghong , Yan Jiye 
Published: February 2023

Abstract
Lasiodiplodia theobromae is a causal agent of Botryosphaeria dieback, which seriously threatens grapevine production worldwide. Plant pathogens secrete diverse effectors to suppress host immune responses and promote the progression of infection, but the mechanisms underlying the manipulation of host immunity by L. theobromae effectors are poorly understood. In this study, we characterized LtCre1, which encodes a L. theobromae effector that suppresses BAX-triggered cell death in Nicotiana benthamiana. RNAi-silencing and overexpression of LtCre1 in L. theobromae showed impaired and increased virulence, respectively, and ectopic expression in N. benthamiana increased susceptibility. These results suggest that LtCre1 is as an essential virulence factor for L. theobromae. Protein–protein interaction studies revealed that LtCre1 interacts with grapevine RGS1-HXK1-interacting protein 1 (VvRHIP1). Ectopic overexpression of VvRHIP1 in N. benthamiana reduced infection, suggesting that VvRHIP1 enhances plant immunity against L. theobromae. LtCre1 was found to disrupt the formation of the VvRHIP1–VvRGS1 complex and to participate in regulating the plant sugar-signaling pathway. Thus, our results suggest that L. theobromae LtCre1 targets the grapevine VvRHIP1 protein to manipulate the sugar-signaling pathway by disrupting the association of the VvRHIP1–VvRGS1 complex.
A survey for variable young stars with small telescopes: VI – Analysis of the outbursting Be stars NSW 284, gaia 19eyy, and VES 263
Authors: Froebrich Dirk , Hillenbrand Lynne A , Herbert Carys , De Kishalay , Eislöffel Jochen , Campbell-White Justyn , Kahar Ruhee , Hambsch Franz-Josef , Urtly Thomas , Popowicz Adam , Bernacki Krzysztof , Malcher Andrzej , Lasota Slawomir , Fiolka Jerzy , Jozwik-Wabik Piotr , Dubois Franky , Logie Ludwig , Rau Steve , Phillips Mark , Fleming George , Farfán Rafael Gonzalez , Alfaro Francisco C Soldán , Nelson Tim , Futcher Stephen R L , Rolfe Samantha M , Campbell David A , Vale Tony , Devine Pat , Moździerski Dawid , Mikołajczyk Przemysław J , Eggenstein Heinz-Bernd , Rodriguez Diego , Walton Ivan L , Vanaverbeke Siegfried , Merrikin Barry , Öğmen Yenal , Perez Alex Escartin , Aimar Mario Morales , Piehler Georg , Dover Lord , Patel Aashini L , Miller Niall , Finch Jack , Hankins Matt , Moore Anna M , Travouillon Tony , Szczepanski Marek 
Published: February 2023

ABSTRACT
This paper is one in a series reporting results from small telescope observations of variable young stars. Here, we study the repeating outbursts of three likely Be stars based on long-term optical, near-infrared, and mid-infrared photometry for all three objects, along with follow-up spectra for two of the three. The sources are characterized as rare, truly regularly outbursting Be stars. We interpret the photometric data within a framework for modelling light-curve morphology, and find that the models correctly predict the burst shapes, including their larger amplitudes and later peaks towards longer wavelengths. We are thus able to infer the start and end times of mass loading into the circumstellar discs of these stars. The disc sizes are typically 3 – 6 times the areas of the central star. The disc temperatures are ∼40 per cent, and the disc luminosities are ∼10 per cent of those of the central Be star, respectively. The available spectroscopy is consistent with inside-out evolution of the disc. Higher excitation lines have larger velocity widths in their double-horned shaped emission profiles. Our observations and analysis support the decretion disc model for outbursting Be stars.
Non-perturbative investigation of low-eccentricity exterior mean motion resonances
Authors: Malhotra Renu , Chen Zherui 
Published: February 2023

ABSTRACT
Mean motion resonances are important in the analysis and understanding of the dynamics of planetary systems. While perturbative approaches have been dominant in many previous studies, recent non-perturbative approaches have revealed novel properties in the low-eccentricity regime for interior mean motion resonances of Jupiter in the fundamental model of the circular planar restricted three-body model. Here, we extend the non-perturbative investigation to exterior mean motion resonances in the low-eccentricity regime (up to about 0.1) and for perturber mass in the range of ∼5 × 10−5 to 1 × 10−3 (in units of the central mass). Our results demonstrate that first-order exterior resonances have two branches at low eccentricity as well as low-eccentricity bridges connecting neighbouring first-order resonances. With increasing perturber mass, higher order resonances dissolve into chaos, whereas low-order resonances persist with larger widths in their radial extent but smaller azimuthal widths. For low-order resonances, we also detect secondary resonances arising from small-integer commensurabilities between resonant librations and the synodic frequency. These secondary resonances contribute significantly to generating the chaotic sea that typically occurs near mean motion resonances of higher mass perturbers.