bluuebunny/crossref_metadata_2025_split huggingface dataset:
The G2-Like gene family in Populus trichocarpa: identification, evolution and expression profiles
Authors: Wu Ruihua , Guo Lin , Guo Leyang , Ma Lehang , Xu Kehang , Du Liang 
Published: March 2023

Abstract
The G2-like (GLK) transcription factors are plant-specific transcription factors (TFs) that perform extensive and significant roles in regulating chloroplast development. Here, genome-wide identification, classification, conserved motifs, cis-elements, chromosomal locations, MCScanX, evolution and expression patterns of the PtGLK genes in the woody model plant Populus trichocarpa were analyzed in detail. In total, 55 putative PtGLKs (PtGLK1-PtGLK55) were identified and divided into 11 distinct subfamilies according to the gene structure, motif composition and phylogenetic analysis. Synteny analysis showed that 22 orthologous pairs and highly conserved microscopy between regions of GLK genes across P. thrchocarpa and Arabidopsis were identified. Furthermore, analysis of the duplication events and divergence times provided insight into the evolutionary patterns of GLK genes. The previously published transcriptome data indicated that PtGLK genes presented distinct expression patterns in various tissues and different stages. Additionally, several PtGLKs were significantly upregulated under the responses of cold stress, osmotic stress, and MeJA and GA treatments, implying that they might take part in abiotic stress and phytohormone responses. Overall, our results provide comprehensive information on the PtGLK gene family and elucidate the potential functional characterization of PtGLK genes in P. trichocarpa.
Spatial-temporal evolution and influencing factors of China's economic development performance under carbon emission constraints
Authors: Xie Zhixiang , Zhao Rongqin , Xiao Liangang , Ding Minglei 
Published: March 2023

Abstract
Background: Guided by the green transformation of economic development, incorporating carbon peaking and carbon neutrality into ecological progress, and accelerating the formation of an industrial and energy structure that saves resources and protects the environment are the intrinsic requirements for China's high-quality economic development. The paper uses the DEA model and Malmquist productivity index to measure the economic development performance of 30 provincial units in mainland China, and summarizes the spatial-temporal evolution characteristics. Then, the Tobit model is used to analyze the influencing factors.
Results: Our results show that: (1) The static performance of economic development generally showed an upward trend from 2008 to 2020, except for Beijing, Jilin, Heilongjiang, Guangdong, Hainan, Ningxia and Xinjiang, most of provinces had different degrees of input redundancy and output insufficiency. (2) The spatial distribution pattern of the static performance under carbon emissions constraint is dominated by the higher and high-level areas. As time goes by, the number of provincial units in different level areas tends to be stable, and the spatial distribution shows staggered layout characteristics. (3) The dynamic performance of economic development shows a downward trend from 2008 to 2016, and an upward trend from 2016 to 2020. The dynamic performance in most of provincial units has realized the transformation from the constraint of technological progress to the constraint of scale efficiency. (4) The urbanization level, economic development level, energy efficiency and the vegetation coverage are the main factors affecting economic development performance, while other factors such as industrialization level, environmental regulation, motorization level, openness and government intervention have no significant effect.
Conclusions: This study suggests that China should adopt industrial structure transformation and upgrading, strengthen environmental regulation, promote new energy vehicles and introduce high-tech industries to improve economic development performance in the future.
Open versus closed reduction internal fixation for lateral condyle humeral fractures in children： a systematic review and meta-analysis
Authors: Zhu Suyue , Zheng Yan , Jiang Yazhou , Yin Hanjun , Zhu Dongsheng 
Published: March 2023

Abstract
Objective: The objective of this meta-analysis was to illustrate the clinical outcomes and safety of two different management options for Song stage 2-4 lateral condyle humeral fractures in children.
Method: In January 2023, a systematic computer-based search was conducted. Data were retrieved for patients with two different management options for lateral condyle humeral fractures in children. The primary endpoints were clinical outcomes based on infection, avascular necrosis, and nonunion. After testing for publication bias and heterogeneity between studies, the data was aggregated for stochastic effect models when necessary.
Results: Eight clinical studies with 742 patients were eventually included in the meta-analysis. There was no significant difference between the closed reduction and percutaneous pinning, and open reduction and internal fixation in terms of the clinical outcomes based on infection, avascular necrosis, and nonunion (P > 0.05).
Conclusions: Closed reduction and percutaneous pinning, as well as open reduction and internal fixation of lateral condyle humeral fractures in children, resulted in similar structural stability and functional outcomes. More high-quality randomized controlled trials are needed to determine this conclusion.
The Effect of Gamma Linoleic Acid on the Expression of c-Fos and Inflammatory Factors on the Formalin-Induced Pain
Authors: rahimi kaveh , Nourishirazi Arman , Delaviz Hamid Reza , Ghotbeddin Zohreh 
Published: March 2023

Abstract
Introduction: The sensation of pain as a pathological entity has always been discussed. The current study was conducted to determine the analgesic effect of gamma linoleic acid (GLA) in the formalin test.
Materials and methods: Formalin was injected into the right hind paw in rats. Pain behaviors were determined as a numerical score for 60 minutes after the injection of formalin. The spinal cord was removed to evaluate the protein expression of C-Fos. Also, the levels of TNF-α and IL-1β were measured in the skin where formalin was injected.
Results: Doses of 100 and 150 mg/kg of GLA could reduce pain perception in the acute phase of the formalin test. Also, GLA 100 and 150 mg/kg reduction of TNF-α and IL-1β induced by formalin compared with the control group. There was elevated c-Fos protein observed in the spinal cord after formalin injection in GLA 100 and 150 mg/kg compared with the control group.
Conclusion: Molecular analysis targeting c-Fos and inflammatory cytokines such as TNF-α and IL-1b also showed an effect of GLA, which matched the results of the behavioral data analysis. These results demonstrated that pretreatment, with GLA, produced antinociceptive effects in the formalin test and may be effective for pain relief.
A comparative proteomic analysis for non-invasive early prediction of hypoxic-ischemic injury in asphyxiated neonates
Authors: Gurtoo Sumrati , Karthikkeyan Gayathree , Behera Santosh Kumar , Kotimoole Chinmaya Narayana , Najar Mohd. Altaf , Modi Prashant Kumar , K.S. Sahana , Pinto Sneha M , A.B. Arun 
Published: March 2023

Abstract
Aim:
Hypoxic Ischemic Encephalopathy (HIE) is one of the principal causes of neonatal mortality and long-term morbidity worldwide. The neonatal signs of mild cerebral injury are subtle, making an early precise diagnosis difficult. Delayed detection, poor prognosis, and lack of specific biomarkers for the disease are increasing mortality rates. In this study, we intended to identify specific biomarkers using comparative proteomic analysis to predict the severity of perinatal asphyxia so that its outcome can also be prevented.
Experimental Design:
A case-control study was conducted on 38 neonates, and urine samples were collected within 24
and 72 hours of life. A tandem mass spectrometry-based quantitative proteomics approach followed by validation via sandwich ELISA was performed.
Results:
The LC-MS/MS-based proteomics analysis resulted in the identification of 1,201 proteins in urine with 229, 244 and 426 being differentially expressed in HIE-1, HIE-2 and HIE-3, respectively. Axon guidance, Diseases of programmed cell death, and Detoxification of reactive oxygen species pathways were significantly enriched in mild HIE vs severe HIE. Of the differentially expressed proteins in various stages of HIE, we chose to validate four proteins- APP, AGT, FABP1, and FN1 via sandwich ELISA. Individual and cumulative ROC curves were plotted. AGT and FABP1 together showed high sensitivity, specificity, and accuracy as potential biomarkers for early diagnosis of HIE
Conclusion:
Establishing putative urinary biomarkers will facilitate clinicians to more accurately screen neonates for brain injury and monitor the disease progression. Prompt treatment of neonates may reduce mortality and neurodevelopmental impairment.
Decreased Neutrophil-to-lymphocyte ratio in Patients with Vitiligo
Authors: Weissmann Sarah , Burrack Nitzan , Golan-Tripto Inbal , Horev Amir 
Published: March 2023

Abstract
Introduction
Vitiligo is a skin disorder characterized by defected melanocytes causing depigmented skin lesions. The role of neutrophil-to-lymphocyte (NLR) and platelet-to-lymphocyte (PLR) ratios as prognostic hematological markers has not yet been studied in a large population. We aimed to investigate these values in patients with mild and severe vitiligo compared to healthy controls.
Methods
This was a single-center retrospective cohort study that included patients of all ages diagnosed with vitiligo between 2005 and 2020. We compared patients with vitiligo to controls matched for age and gender.
Results
8,398 adults and 1,428 children were included in this study. Patients had a lower NLR compared to their controls (2.04 vs 2.23, p<0.001). There was no significant difference in PLR between patients and controls. We found no significant differences in NLR and PLR between mild and severe vitiligo patients. Patients with mild vitiligo had a mean NLR of 2.04 while their controls had a mean NLR of 2.22 (p-value<0.001). Patients with severe vitiligo had a mean NLR of 2.01 while their matched controls had a mean NLR of 2.27 (p-value<0.001).
Conclusion
Decreased NLR is associated with both severe and mild forms of vitiligo.
Suv39h1 silencing recovers memory decline due to scopolamine-induced amnesia
Authors: Kushwaha Akanksha , Thakur MK 
Published: March 2023

Abstract
Histone post-translational modifications play an important role in the regulation of long-term memory and modulation of expression of neuronal immediate early genes (IEGs). The histone methyltransferase Suv39h1 (a mammalian ortholog of the Drosophila melanogaster SU(VAR) 3–9) aids in the methylation of histone H3 at lysine 9. We previously reported that age-related memory decline is associated with an increase in Suv39h1 expression in the hippocampus of male mice. The scopolamine-induced amnesic mouse model is a well-known animal model of memory impairment. In the current study, we have made an attempt to find a link between the changes in H3K9 trimethylation pattern and memory decline during scopolamine-induced amnesia. It was followed by checking the effect of siRNA-mediated silencing of hippocampal Suv39h1 on memory and expression of neuronal IEGs. Scopolamine treatment significantly increased global levels of H3K9me3 and Suv39h1 in the amnesic hippocampus. Suv39h1 silencing in amnesic mice reduced H3K9me3 levels at the neuronal IEGs (Arc and BDNF) promoter, increased the expression of Arc and BDNF in the hippocampus, and improved recognition memory. Thus, these findings suggest that the silencing of Suv39h1 alone or in combination with other epigenetic drugs might be effective for treating memory decline during amnesia.
Feasibility and acceptability of group CBT+CRT for adults with major depressive disorders in an Iranian community: a single group study
Authors: Khodabandeloo Yusuf , Sohrabi Faramarz , Borjali Ahmad , Isamorad Abolghasem , Motamedi Abdollah 
Published: March 2023

Abstract
Background Despite significant advances in the psychological treatment of Major Depressive Disorder (MDD), many treatment challenges remain, such as high recurrence and functional impairment after treatment. It seems that ignoring underlying factors such as cold cognitive dysfunctions plays an important role in these therapeutic challenges. Although a lot of research has been done on hot cognition, cold cognition is about neglect. Therefore, the purpose of this study was to assess the feasibility and acceptability and to estimate the effect size of the group CBT+CRT for future controlled trials.
Methods Among the 31 volunteers who were diagnosed with MDD in the online clinical interview, 14 adults aged 22-40 years were treated in 12 weekly two-hour sessions. Only 12 patients remained in the study at week 12. Psychological and neuropsychological assessments were evaluated by the Beck Depression Inventory-Second Edition (BDI-II), the Snaith-Hamilton Pleasure Scale (SHAPS), and the THINC-it tool.
Results Data analysis showed that psychological symptoms of depression were significantly reduced at post-test and at follow-up compared to pre-test based on BDI-II and SHAPS scores. Regarding neuropsychological symptoms, effect sizes were largest for attention (d=−1.37) and processing speed (d=−1.18), but smaller effect sizes were observed for working memory (d=−0.26) and executive functioning (d=−0.54). The observed effect sizes remained relatively stable at the three-month follow-up.
Conclusion To the authors' knowledge, this is the first study to evaluate group CBT+CRT for adults with MDD designed based on the hot-cold cognition model of depression, stimulating further developmental research. In general, the results showed the sufficient feasibility and acceptance of this treatment manual.
Alkali-Tolerant Bacillus Producing PHA and Application of its PHA Surface Binding Protein as Surfactant
Authors: Fan Xueyu , Fu Shuangqing , Jiang Junpo , Liu Dexu , Li Xinyue , Han Mengnan , Li Wei , Zhang Honglei 
Published: March 2023

Abstract
Polyhydroxyalkanoates (PHA) produced by Bacillus bacteria have potential advantages in medical applications, due to its cell membrane do not contain outer membrane lipopolysaccharide (LPS). However, the PHA accumulation level is far lower than that of Gram-negative bacteria. In this study, an alkali-tolerant Bacillus cereus HBL-AI was screened using Nile Red and Sudan Black B in a Low-Salt LB solid medium at pH 8.0. This strain displayed high PHA accumulation capacity in fed‑batch fermentation with glucose as the carbon source in a 5 L unsterilized fermentor. The highest PHA accumulation reached 12.8 g/L and the extracted product was finally determined as (Poly-β-3-hydroxybutyrate, PHB). It has the potential to be used for large-scale open and continuous fermentation. Furthermore, PHA membrane binding protein (PhaP), PHA regulatory protein (PhaQ and PhaR) derived from HBL-AI were heterologously expressed inE. coli and purified to investigate their application as biosurfactants. The results showed that the emulsification ability and stability of three amphiphilic proteins were higher than those of widely used chemical surfactants. The protein concentration of PhaQ used to form a stable emulsion layer in vegetable oil was the lowest (50 μg/mL), which greatly reduced the amount of protein used in emulsification. This clearly demonstrated that HBL-AI has great potential in the production of PHA without endotoxin, and its PHA binding protein can be well applied as an environmentally friendly biosurfactants.
WITHDRAWN: An Electric-Field High Energy Harvester from Medium voltage power line with parallel line
Authors: Srila Aekanuwat , Hoyingchareon Pakpoom , Prasertsit Anuwat , Chetpattananondh Kanadit 
Published: March 2023

Abstract
In order to effectively monitor transmission lines and transmission towers, a number of different types of sensors are needed. A lot of times, these sensors along with the transmission lines and transmission towers are in inaccessible or hard-to-access areas and replacing their batteries is difficult. Yet they need consistent power supply. By harvesting energy directly from these medium and high voltage power lines, these devices can become self-sustaining while the overall system is more friendly to the environment. This paper presents a novel approach to high energy harvesting based on capacitive coupling between the power line and the harvesting line. This technique has several advantages, namely high output voltage, easy adjustment of coupling coefficient, and low cost. The validation and implantation of this harvesting system are proposed with the support of experimental results. This energy-harvesting ability of W and mA levels is achieved for the power line monitoring devices, with higher power output depending on the length and the size of the harvesting line.
Apple Leaf Disease Recognition with Segmentation Research Trend: A Systematic Analysis Using VoSViewer
Authors: Bonkra Anupam , Bhatt Pramod kumar , Kaur Amandeep , Shah Mohd Asif 
Published: March 2023

Abstract
The study’s goals were to examine the effects of unforeseen weather circumstances on agricultural productivity and to test artificial intelligence-based strategies for feature extraction and segmentation for plant leaf disease identification. A bibliometric analysis was used in the study to look into current trends, publications, citation structures, patterns of ownership and collaboration, production patterns, and other aspects connected to the early detection of apple diseases. While there have been many studies on recognising apple illnesses, there have been few attempts to develop a thorough multidisciplinary science map because disease detection is not exclusive to any one field of study. The study’s objectives were to combine knowledge frameworks and identify the current state of the research field. A scientometric examination of 200 papers from the Scopus database between 2017 and 2022 was done by the researchers in order to accomplish this goal. VOSviewer was utilised for the research, and its automated workflow was used to pick significant journals, authors, nations, articles, and themes. To find patterns and trends, social network studies, citation and co-citation checks, and citation counts were all carried out. The study revealed changes in the cognitive and social structure of the area and concluded that the interdisciplinary field of sickness detection employing segmentation has expanded over time. The study also revealed areas that need additional investigation and clarified the intellectual underpinnings of the topic.
Comprehensive bioinformatics analysis to identify a novel Ferroptosis-related gene and its ceRNA regulatory network and candidate Therapeutic Agents in UVB-irradiated human skin
Authors: Huang Qilong , Li Guyu , Han Jiayu , Wang Zhaohua , Li Shuo 
Published: March 2023

Abstract
UVB light can be toxic to the skin, penetrating the epidermal layer and potentially causing skin disease. Ferroptosis is an iron-dependent form of regulated cell death that differs from apoptosis. To better understand the molecular biomarkers and potential mechanisms of UVB-induced skin damage with Ferroptosis-related genes, we downloaded two microarray datasets (GSE45493 and GSE56754) from Gene Expression Omnibus (GEO) and performed bioinformatic analyses. By analyzing differential gene expression with the Limma package, we identified 35 DEGs (28 up-regulated and 7 down-regulated) and used Cytoscape 3.8.2 to screen for hub genes based on the degree of connectivity in the PPI network. IL6 was obtained by intersecting genes related to ferroptosis. We then proposed the ceRNA network of IL6 by searching multiple online miRNA and lncRNA databases. Finally, we screened 7 significant potential activating compounds using the CTD platform, including benzo(a)pyrene, bardoxolone methyl, tetrachlorodibenzodioxin, testosterone undecanoate, demecolcine, tetradecanoylphorbol acetate, and cyclosporine. Molecular docking experiments were conducted to verify the reliability of these potential drugs. This study provides insights into the molecular mechanism of UVB-irradiated human skin and identifies potential candidate drugs for treating it.
Effects of Socio-economic Status and Daily Variation on Municipal Solid Waste Composition: A Preliminary Analysis for Sustainable Planning and Development in Aligarh City
Authors: Singh Alok , Srivast Amitabh Kumar 
Published: March 2023

Abstract
In context of developing countries such as India, with great differences in living standards of people, different communities, MSW management is one of the most promising problems in front of municipal organizations. Unlike every city in India, Aligarh city is also facing the same problem of MSWM. This problem not only affects the aesthetic view but also it is hazardous to health of people nearby. Currently, solid waste collected is either dumped in landfill unscientifically or partially treated by A to Z waste management (limited) by composting. In the present study, an effort was made to know about the per capita waste generation and variations in the quantity of different components of the MSW in five different regions of the city of different living standards. Also, weekly variation was also analyzed in tie study. One Way Anova analysis using SPSS is performed to analyze the variations in the mean composition of different components. The per capita solid waste generation in Aligarh city was found to be 0.42 kg/person/day. From the analysis, we came to know that compostable component (35.4%) is the highest, then inert (24.6%), plastic (12.2%), paper (10%), textile (9.2%), sand (8.6%). After analysis, the results can be helpful in sorting out the problem of MSW management in the city by selecting appropriate units as per composition of MSW.
E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation
Authors: Levin Roman , Löhr Frank , Karakoc Betül , Lichtenecker Roman , Dötsch Volker , Bernhard Frank 
Published: March 2023

Abstract
Cell-free (CF) synthesis with highly productive E. coli lysates is a convenient method to produce labeled proteins for NMR studies. Despite reduced metabolic activity in CF lysates, a certain scrambling of supplied isotope labels is still notable. Most problematic are conversions of 15N labels of the amino acids L-Asp, LAsn, L-Gln, L-Glu and L-Ala, resulting into ambiguous NMR signals as well as into label dilution. Specific inhibitor cocktails could suppress most undesired conversion reactions, while limited availability and potential side effects on CF system productivity need to be considered. As alternative route to address NMR label conversion in CF systems, we describe the generation of optimized E. coli lysates with reduced amino acid scrambling activity. Our strategy is based on the proteome blueprint of standardized CF S30 lysates of the E. coli strain A19. Identified lysate enzymes with suspected amino acid scrambling activity were eliminated by constructing corresponding single and cumulative chromosomal mutations in A19. CF lysates prepared from the mutants were analyzed for their CF protein synthesis efficiency and for residual scrambling activity. The A19 derivative “Stablelabel” containing the cumulative mutations asnA, ansA/B, glnA, aspC and ilvE was finally identified as giving the most useful CF S30 lysates. We demonstrate the optimized NMR spectral complexity of selectively labeled proteins CF synthesized in “Stablelabel” lysates. By taking advantage of the ilvE deletion in "Stablelabel", we further exemplify a new strategy for methyl group specific labeling of membrane proteins with the proton pump proteorhodopsin.