bluuebunny/crossref_metadata_2025_split huggingface dataset:
GASTROINTESTINAL ANGIONEUROTIC EDEMA AS A CONSEQUENCE OF ANGIOTENSIN – CONVERTING ENZYME INHIBITOR TREATMENT
Authors: Wojciechowska Ewa , Gryglas Piotr , Dul Pawel 
Published: June 2023

Objective:
To report a case of unusual severe adverse reaction to an angiotensin-converting enzyme inhibitor (ACEI).

Design and method:
A 33-year-old male with a history of hypertension for 10 years, asthma, and atopy was admitted to a local emergency department due to diffuse abdominal pain with vomiting and diarrhea for about 3 days. A few days earlier, the patient had blood pressure (BP) lowering treatment modified due to suboptimal BP control, with introduction of perindopril. The first ACEI dose was followed by lower abdominal pain, urge to have bowel movement, and difficulty passing stool. 24 hours later, the next dose was followed by severe, gradually increasing, diffuse abdominal pain, nausea, vomiting, and diar-rhea. With signs of peritonitis present on admission, a surgeon suspected an acute abdomen and or-dered abdominal computed tomography (CT) due to unclear etiology. CT showed diffuse infiltrates within the small intestine and its mesentery, with free abdominal fluid but no perforation. The patient was not operated and seen by an internist who suspected gastrointestinal angioneurotic edema related to ACEI. On admission to the medical unit, the patient was moderately unwell, conscious, with BP 185/110 mmHg, HR 128 bpm, SaO2 99% on room air, and no fever. Abdomen examination showed reduced bowel sounds, abdominal guarding, tenderness on palpation, especially in the mid-abdomen, and rebound tenderness. Laboratory tests showed elevated WBC (16,800/mm3) and CRP (19 mg/L).

Results:
Perindopril was withdrawn, the patient received antihistamines, analgesics, and intravenous fluids, with improvement of the patient's condition and reduction of pain and inflammation markers. Follow-up abdominal CT after 5 days showed resolution of small intestinal edematous lesions. The patient was discharged home in good condition, without abdominal pain, and with good BP control. The final diag-nosis was gastrointestinal angioneurotic edema associated with ACEI treatment.

Conclusions:
One condition may mimic another and thus unclear clinical presentations require cautious diagnostic and therapeutic decisions. In the presented case, angioneurotic edema following introduction of perin-dopril was unusually located in the gastrointestinal tract and its manifestations imitated an acute abdo-men.
CLINICAL INERTIA AND ADHERENCE IN THE CROATIAN RURAL AREA DAMAGED BY AN EARTHQUAKE. “THE SILENT KILLER HUNT”-CROATIAN SOCIETY OF HYPERTENSION PROJECT
Authors: Sovic Ariana , Abramovic Inga , Lonic Jakov , Mestrovic Dora , Petrinovic Martina , Strabic Adrijana , Jelakovic Bojan 
Published: June 2023

Objective:
Adherence to medical advice is a major factor in treating hypertension. Motivation to implement lifestyle changes is as important as taking medications. To gain a better understanding of patient compliance our aim was to find out patients’ adherence to physicians’ medical advice.

Design and method:
A survey was conducted on a sample of 214 subjects as a part of a project ’The Silent Killer Hunt’, which targets populations at risk for developing hypertension (opportunistic screening). The participants were given a questionnaire containing two types of questions, the first one being about the recommendation they were or were not given by their general practitioner, and the second one about their subsequent change of behavior. Jamovi was used for statistical analysis.

Results:
A total of 94.7% have received a recommendation to use medication for hypertension and 106 (49.5%) people have confirmed that they take their medication regularly. On the other hand, only 46.5% of the surveyed participants have been advised to decrease their body weight. Increasement in physical activity was suggested to 55.3% and a total of 30.2% and 28.6%, have not been successful in reducing their body weight and increasing their physical activity, respectively. More than half of the subjects (63.2%) were educated about lowering their salt intake.

Conclusions:
Clinical inertia and poor adherence were found in this rural area. Education of physicians and nurses is equally important as the education of patients and according to our results, it is even more important in this remote and poor part of Croatia.
Surgical Ergonomics and Preventing Work-Related Musculoskeletal Disorders
Authors: Yurteri-Kaplan Ladin A. , Park Amy J. 
Published: June 2023

(Abstracted from Obstet Gynecol 2023;141(3):455–462
A busy surgical career requires many intense years of training, practice, and education. The authors of this article postulate an urgent need for a significant paradigm shift to viewing surgeons as high-performance athletes, with a need for a new concept for their work environment to ensure mental acuity, and ergonomic changes to preserve physical dexterity and longevity.
A Phase III Study of Pafolacianine Injection (OTL38) for Intraoperative Imaging of Folate Receptor–Positive Ovarian Cancer (Study 006)
Authors: Tanyi Janos L. , Randall Leslie M. , Chambers Setsuko K. , Butler Kristina A. , Winer Ira S. , Langstraat Carrie L. , Han Ernest S. , Vahrmeijer Alexander L. , Chon Hye Sook , Morgan Mark A. , Powell Matthew A. , Tseng Jill H. , Lopez Alexis S. , Wenham Robert M. 
Published: June 2023

(Abstracted from J Clin Oncol 2022;41:276–284
Complete resection of macroscopic disease remains a key focus of cytoreductive surgery for the treatment of ovarian cancer. Barriers to achieving complete resection include small-sized lesions, obscuring tissue damage from prior chemotherapy, and incomplete exploration.
Referred sensations in the orofacial region are associated with a decreased descending pain inhibition and modulated by remote noxious stimuli and local anesthesia
Authors: Sago Teppei , Costa Yuri M. , Ferreira Dyna M. , Svensson Peter , Exposto Fernando G. 
Published: June 2023

Abstract
Referred sensation (RS) as a specific clinical phenomenon has been known for a long time, although the underlying mechanisms remain unclear. The aims of this study were to assess if (1) healthy individuals who experienced RS had a less active endogenous pain system when compared with those who did not; (2) activation of descending pain inhibition mechanisms can modulate RS parameters; and finally, (3) a transient decrease in peripheral afferent input because of a local anesthetic (LA) block in the masseter muscle can modulate RS parameters. To assess these, 50 healthy participants were assessed in 3 different sessions. In the first session, conditioned pain modulation (CPM) as well as mechanical sensitivity and RS at the masseter muscle were assessed. In the same session, participants who experienced RS had their mechanical sensitivity and RS assessed again while undergoing a CPM protocol. In the second and third sessions, participants had their mechanical sensitivity and RS assessed before and after receiving an injection of 2 mL of LA and isotonic saline into the masseter muscle. The main findings of this study were (1) participants who experienced RS during standardized palpation exhibited increased mechanical sensitivity (P < 0.05, Tukey post hoc test) and decreased CPM (P < 0.05, Tukey post hoc test) when compared with those who did not; RS incidence (P < 0.05, Cochran Q test), frequency (P < 0.05; Friedman test), intensity (P < 0.05, Tukey post hoc test), and area (P < 0.05, Tukey post hoc test) were all significantly reduced when assessed (2) during a painful conditioning stimulus and (3) after LA block. These novel findings highlight that RS in the orofacial region are strongly modified by both peripheral and central nervous system factors.
Application of metagenomic sequencing of drainage fluid in rapid and accurate diagnosis of postoperative intra-abdominal infection: a diagnostic study
Authors: Zhu Ruizhe , Hong Xiafei , Zhang Dong , Xiao Yi , Xu Qiang , Wu Bin , Guo Junchao , Han Xianlin , Yang Qiwen , Zhao Yupei , Wu Wenming 
Published: June 2023

Background:
Postoperative intra-abdominal infection (PIAI) is one of the most serious complications of abdominal surgery, increasing the risk of postoperative morbidity and mortality and prolonging hospital stay. Rapid diagnosis of PIAI is of great clinical value. Unfortunately, the current diagnostic methods of PIAI are not fast and accurate enough.

Methods:
We performed an exploratory study to establish a rapid and accurate diagnostic method of PIAI. We explored the turnaround time and accuracy of metagenomic next-generation sequencing (mNGS) in diagnosing PIAI. Patients who underwent elective abdominal surgery and routine abdominal drainage with suspected PIAI were enrolled in the study. The fresh midstream abdominal drainage fluid was collected for mNGS and culturing.

Results:
We found that the median sample-to-answer turnaround time of mNGS was dramatically decreased than that of culture-based methods (less than 24 h vs. 59.5 to 111 h). The detection coverage of mNGS was much broader than culture-based methods. We found 26 species from 15 genera could only be detected by mNGS. The accuracy of mNGS was not inferior to culture-based methods in the 8 most common pathogens detected from abdominal drainage fluid (sensitivity ranged from 75% to 100%, specificity ranged from 83.3% to 100%, and kappa values were higher than 0.5). Moreover, the composition of the microbial spectrum established by mNGS varied between upper and lower gastrointestinal surgery, enhancing the understanding of PIAI pathogenesis.

Conclusion:
This study preliminarily revealed the clinical value of mNGS in the rapid diagnosis of PIAI and provided a rationale for further research.
Group vs Traditional Prenatal Care for Improving Racial Equity in Preterm Birth and Low Birthweight: The Centering and Racial Disparities Randomized Clinical Trial Study
Authors: Crockett Amy H. , Chen Liwei , Heberlein Emily C. , Britt Jessica L. , Covington-Kolb Sarah , Witrick Brian , Doherty Emily , Zhang Lu , Borders Ann , Keenan-Devlin Lauren , Smart Britney , Heo Moonseong 
Published: June 2023

(Abstracted from Am J Obstet Gynecol 2022;227:893.e1–893.e15
Preterm birth (PTB) is far too common in the United States and disproportionately affects Black individuals. To address these differences, a group care model was proposed that would provide health care with family and peer support and provider-led patient-centered health education.
The Use of Flap Techniques to Preserve Limb Length in Patients with Transmetatarsal Amputations
Authors: Deldar Romina , Sayyed Adaah A. , Cach Gina , Choi Christopher , Garada Firras , Atves Jayson N. , Steinberg John S. , Fan Kenneth L. , Attinger Christopher E. , Evans Karen K. 
Published: June 2023

Background:
Chronic lower extremity (LE) wounds affect up to 13% of the United States population. Transmetatarsal amputation (TMA) is frequently performed in comorbid patients with chronic forefoot wounds. TMA allows limb salvage and preserves functional gait, without need for prosthesis. Traditionally, when tension-free primary closure is not possible, a higher-level amputation is performed. This is the first series to evaluate the outcomes following local and free flap coverage of TMA stumps in patients with chronic foot wounds.

Methods:
A retrospective cohort of patients who underwent TMA with flap coverage from 2015-2021 were reviewed. Primary outcomes included flap success, early postoperative complications, and long-term outcomes (limb salvage and ambulatory status). Patient-reported outcome measures using the lower extremity functional scale (LEFS) were also collected.

Results:
Fifty patients underwent 51 flap (26 local, 25 free flap) reconstructions following TMA. Average age and BMI were 58.5 years and 29.8 kg/m2, respectively. Comorbidities included diabetes (n=43, 86%) and peripheral vascular disease (n=37, 74%). Flap success rate was 100%. At mean follow-up of 24.8 months (range, 0.7 to 95.7 months), the limb salvage rate was 86.3% (n=44). Forty-four patients (88%) were ambulatory. The LEFS survey was completed by 24 surviving patients (54.5%). Mean LEFS score was 46.6 ± 13.9, correlating with 58.2 ± 17.4% of maximal function.

Conclusion:
Local and free flap reconstruction following TMA are viable methods of soft tissue coverage for limb salvage. Applying plastic surgery flap techniques for TMA stump coverage allows for preservation of increased foot length and ambulation without a prosthesis.
Auditory and cognitive function in older adults living with and without HIV
Authors: Torre Peter , Sundermann Erin E. , Brandino Amanda , Heaton Anne , Devore Julia , Anderson Albert M. , Moore Raeanne C. 
Published: June 2023

Objectives:
To evaluate: 1) the peripheral hearing sensitivity and central auditory processing in persons living with HIV (PWH) and persons living without HIV (PWoH); and 2) the association between cognitive function and central auditory processing in PWH and PWoH.

Design:
Cross-sectional, observational study.

Methods:
Participants included 67 PWH (70.2% male; mean age = 66.6 years [SD = 4.7 years]) and 35 PWoH (51.4% male; mean age = 72.9 years [SD = 7.0 years]). Participants completed a hearing assessment and a central auditory processing assessment that included dichotic digits testing (DDT). Pure-tone air-conduction thresholds were obtained at octave frequencies from 0.25 through 8 kHz. A pure-tone average (PTA) was calculated from 0.5, 1, 2, and 4 kHz thresholds for each ear. Participants also completed a neuropsychological battery assessing cognition in seven domains.

Results:
PWH had slightly lower (i.e., better) PTAs compared to PWoH, but this was not statistically significant. Conversely, PWH and PWoH had similar DDT results for both ears. Poorer verbal fluency, learning, and working memory performance was significantly related to lower DDT scores, and those defined as having verbal fluency, learning, and working memory impairment had significantly poorer DDT scores (8–18% lower) in both ears.

Conclusions:
Hearing and DDT results were similar in PWH and PWoH. The relationship between verbal fluency, learning, and working memory impairment and poorer DDT results did not differ by HIV serostatus. Clinicians, particularly audiologists, should be mindful of cognitive functioning abilities when evaluating central auditory processing.
PICNIC accurately predicts condensate-forming proteins regardless of their structural disorder across organisms
Authors: Hadarovich Anna , Singh Hari Raj , Ghosh Soumyadeep , Rostam Nadia , Hyman Anthony A. , Toth-Petroczy Agnes 
Published: June 2023

AbstractBiomolecular condensates are membraneless organelles that can concentrate hundreds of different proteins to operate essential biological functions. However, accurate identification of their components remains challenging and biased towards proteins with high structural disorder content with focus on self-phase separating (driver) proteins. Here, we present a machine learning algorithm, PICNIC (Proteins Involved in CoNdensates In Cells) to classify proteins involved in biomolecular condensates regardless of their role in condensate formation. PICNIC successfully predicts condensate members by identifying amino acid patterns in the protein sequence and structure in addition to the intrinsic disorder and outperforms previous methods. We performed extensive experimental validationin celluloand demonstrated that PICNIC accurately predicts 21 out of 24 condensate-forming proteins regardless of their structural disorder content. Even though increasing disorder content was associated with organismal complexity, we found no correlation between predicted condensate proteome content and disorder content across organisms. Overall, we applied a novel machine learning classifier to interrogate condensate components at single protein and whole-proteome levels across the tree of life (picnic.cd-code.org).
A Deep Learning Approach for Mental Health Quality Prediction Using Functional Network Connectivity and Assessment Data
Authors: Ajith Meenu , Aycock Dawn M. , Tone Erin B. , Liu Jingyu , Misiura Maria B. , Ellis Rebecca , King Tricia Zawacki , Dotson Vonetta M. , Calhoun Vince 
Published: June 2023

AbstractWhile one can characterize mental health using questionnaires, such tools do not provide direct insight into the underlying biology. By linking approaches that visualize brain activity to questionnaires in the context of individualized prediction, we can gain new insights into the biology and behavioral aspects of brain health. Resting-state fMRI (rs-fMRI) can be used to identify biomarkers of these conditions and study patterns of abnormal connectivity. In this work, we estimate mental health quality for individual participants using static functional network connectivity (sFNC) data from rs-fMRI. The deep learning model uses the sFNC data as input to predict four categories of mental health quality and visualize the neural patterns indicative of each group. We used guided gradient class activation maps (guided Grad-CAM) to identify the most discriminative sFNC patterns. The effectiveness of this model was validated using the UK Biobank dataset, in which we showed that our approach outperformed four alternative models by 4-18% accuracy. The proposed model’s performance evaluation yielded a classification accuracy of 76%, 78%, 88%, and 98% for the excellent, good, fair, and poor mental health categories, with poor mental health accuracy being the highest. The findings show distinct sFNC patterns across each group. The patterns associated with excellent mental health consist of the cerebellar-subcortical regions, whereas the most prominent areas in the poor mental health category are in the sensorimotor and visual domains. Thus the combination of rs-fMRI and deep learning opens a promising path for developing a comprehensive framework to evaluate and measure mental health. Moreover, this approach had the potential to guide the development of personalized interventions and enable the monitoring of treatment response. Overall this highlights the crucial role of advanced imaging modalities and deep learning algorithms in advancing our understanding and management of mental health.
Boldine alters serum lipidomic signatures after acute spinal cord transection in male mice
Authors: Graham Zachary A. , Siedlik Jacob A. , Toro Carlos A. , Harlow Lauren , Cardozo Christopher P. 
Published: June 2023

AbstractTraumatic spinal cord injury (SCI) results in wide-ranging cellular and systemic dysfunction in the acute and chronic time frames after the injury. Chronic SCI has well-described secondary medical consequences while acute SCI has unique metabolic challenges as a result of physical trauma, in-patient recovery and other post-operative outcomes. Here, we used high resolution mass spectrometry approaches to describe the circulating lipidomic and metabolomic signatures using blood serum from mice 7 d after a complete SCI. Additionally, we probed whether the aporphine alkaloid, boldine, was able to prevent SCI-induced changes observed using these ‘omics platforms. We found that SCI resulted in large-scale changes to the circulating lipidome but minimal changes in the metabolome, with boldine able to reverse or attenuate SCI-induced changes in the abundance of 50 lipids. Multiomic integration using xMWAS demonstrated unique network structures and community memberships across the groups.
Bench-ML: A Benchmarking Web Interface for Machine Learning Methods and Models in Genomics
Authors: Rene Lopez , Mario Makita , Laura Ortega , Avantika Lal , Hernan De La Garza 
Published: June 2023

AbstractMachine learning is a complex but essential technology in genomics data analysis and its popularity has increased the rate of new methodological approaches published but this raises the question of how models should be benchmarked and validated.Bench-ML is a generalizable and easy to use web interface for benchmarking and validation that can preprocess data, train, test, evaluate and compare machine learning algorithms for genomics. It makes benchmarking machine learning methods more accessible by enabling genomics scientists to perform end-to-end analyses, visualize results and evaluate performance or metrics to compare methods and models by providing a point of reference using only a web browser.To improve something it needs to be measured; To benchmark and evaluate models Bench-ML provides several strategies, methodologies, and tools to generate measurements and visualizations to track experiments to help identify areas of opportunity using metrics such as loss and accuracy, model visualization, learning and saturation curves, principal component analysis, feature scoring, confusion matrix, regression for training and test data, mean absolute error, etc.Bench-ML explains the different options to test and validate machine and deep learning models to identify problematic areas and potentially improve performance. Bench-ML provides several strategies to improve performance like showing when a model is not performing or when different hyperparameters values could be needed, it also helps fine tune hyperparameter values and to identify accuracy across multiple classes and from these classes which class could affect performance.The selection, development, and comparison of machine learning methods and models in genomics datasets can be a daunting task based on the goals of a particular study or the target problem. Machine learning is very good at pattern recognition but modeling the world is much more than that so how to know if a machine learning method or model is performing at a good sensitivity and specificity in large genomics datasets is still a big problem and this is where Bench-ML can help.
PTP1B phosphatase puts a brake on iPSC-derived neutrophil motility and antimicrobial function
Authors: Giese Morgan A. , Bennin David A. , Schoen Taylor J. , Peterson Ashley N. , Brand Josh , Jung Ho Sun , Dinh Huy Q. , Slukvin Igor I. , Huttenlocher Anna 
Published: June 2023

AbstractNeutrophils are rapidly recruited to sites of infection and are critical for pathogen clearance. Neutropenic patients are at high risk for fungal and bacterial infections and can benefit from granulocyte transfusion therapy. Human induced pluripotent stem cells (iPSCs) could provide a robust source of neutrophil-like cells for infusion as they can be generated in large quantities and do not require a donor. However, dampened intracellular signaling limits their cellular activation and response. Here, we show that we can engineer iPSC-derived neutrophils (iNeutrophils) for enhanced motility and anti-microbial functions. Deletion of the PTP1B phosphatase increased iNeutrophil PI3K and ERK signaling and was associated with increased F-actin polymerization, cell migration and phagocytosis. PTP1B deletion also increased production of inflammatory cytokines, including the neutrophil chemoattractant IL-8. Furthermore, PTP1B-KO iNeutrophils displayed a highly activated morphology and were more responsive to the fungal pathogenAspergillus fumigatus. KO iNeutrophils efficiently migrated to and swarmed hyphae resulting in inhibition of fungal growth. Taken together, deletion of the PTP1B phosphatase removes the “brakes” on iPSC-derived intracellular signaling and neutrophil function.Key pointsDeletion of PTP1B increases iPSC-derived neutrophil intracellular signaling to improve motility and phagocytosis.Deletion of PTP1B enhances iPSC-derived neutrophil swarming response and ability to inhibit fungal growth.