bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of hnRNP A1 Splicing Inhibition on the Brain Remyelination Proteome
Authors: Brandão‐Teles Caroline , Carregari Victor Corasolla , Reis‐de‐Oliveira Guilherme , Smith Bradley J. , Chaves Yane , Sousa Santos Aline Valéria , Pinheiro Erick Martins de Carvalho , Oliveira Caio C. , Vieira Andre Schwambach , Crunfli Fernanda , Martins‐de‐Souza Daniel 
Published: January 2025

ABSTRACTOligodendrocytes, the myelinating cells in the central nervous system, are implicated in several neurological disorders marked by dysfunctional RNA–binding proteins (RBPs). The present study aimed at investigating the role of hnRNP A1 in the proteome of the corpus callosum, prefrontal cortex, and hippocampus of a murine cuprizone–induced demyelination model. Right after the cuprizone insult, we administered an hnRNP A1 splicing activity inhibitor and analyzed its impact on brain remyelination by nanoESI‐LC‐MS/MS label‐free proteomic analysis to assess the biological processes affected in these brain regions. Significant alterations in essential myelination proteins highlighted the involvement of hnRNP A1 in maintaining myelin integrity. Pathways related to sphingolipid and endocannabinoid signaling were affected, as well as the synaptic vesicle cycle and GABAergic synapses. Although behavioral impairments were not observed, molecular changes suggest potential links to memory, synaptic function, and neurotransmission processes. These findings enhance our understanding of the multifaceted roles of hnRNP A1 in the central nervous system, providing valuable insights for future investigations and therapeutic interventions in neurodegenerative and demyelinating diseases.image
An International Survey on the Use of a Polyrevitalizing Solution With or Without Other Aesthetic Procedures in the Daily Practice of Aesthetic Physicians
Authors: Fanian Ferial , Casabona Gabriela , Bartoletti Emanuele , Cartier Hugues , Landau Marina , Galadari Hassan , Bageorgou Fotini , Ogilvie Alexandra , Theodorakopoulou Elina , Arteaga Ariana , Gokalp Hilal , Pisal Philippe Hamida , Lopez Ingrid Gerhke , Rucinski Gabriel , Chebbah Myriam , Le Clanche Solenn , Varlet Alice‐Anaïs , Sukmanskaya Natalia , Issa Hanane , Philippon Valerie , Stratigos Alexander 
Published: January 2025

ABSTRACTObjectiveNCTF135HA, a versatile polyrevitalizing solution, is a potent agent for enhancing skin quality, radiance, moisture, vitality, and diminishing fine wrinkles caused by aging factors. Data demonstrate a divergence in its application from skin quality enhancement to treatment of vitiligo lesions. To know more precisely about the protocol of use among providers, alone or in combination with other procedures, we performed an international survey.MethodA Steering Committee of dermatologists, aesthetic surgeons, and physicians developed a 32‐question questionnaire based on a literature review. Hosted online from January to March 2024, it targeted healthcare professionals experienced in polyrevitalization. Responses were analyzed anonymously and reported descriptively.ResultsPractitioners adopt a balanced approach: half of their patients receiving classic Polyrevitalization (NCTF135HA alone) and the other half undergoing combination therapy (NCTF135HA with another aesthetic procedure). Most administer NCTF135HA across multiple sessions, typically three (39.7%). In combination therapy, 55.5% of practitioners use NCTF135HA for medical purposes, targeting Melasma (53.1%) and Rosacea (45.0%) for instance. Aesthetic use is prevalent, with 94.2% employing NCTF135HA for skin wrinkles (79.9%), and refreshment, rejuvenation, and hydration (73.4%). Combining NCTF135HA with hyaluronic acid (71.2%) and botulinum toxin (49.1%) is common, alongside microneedling (50.2%), peelings (32.4%), and fractional radiofrequency (25.6%).Limitations and ConclusionOur survey showed a homogenous distribution of NCTF135HA utilization, indicating a consensus on its application across diverse demographics. This consistency highlights its widespread acknowledgment and efficacy in various aesthetic and clinical settings. Limitations include a large respondent group from Mexico and many respondents with less than 10 years of experience.
A Systematic Literature Review on Auction Mechanisms: Insights From the Last Decade and Future Directions
Authors: Felicetti Alberto Michele , Luzzi Martina , Ammirato Salvatore , Guerriero Francesca 
Published: January 2025

ABSTRACTThis study reports the results of a systematic literature review on auctions mechanism. Auctions are a very popular practice employed in many fields but does not exist a research that investigates the use of auctions under a cross‐disciplinary approach. This work is focused on analyzing which are the areas where auctions are mostly adopted and what are the techniques used to solve auction‐related problems. Referring to the publications from 2013 to 2024 and using the latent Dirichlet allocation (LDA) algorithm, three main topics related to auctions research are identified and widely explained. In addition, an auction classification scheme in which auction characteristics are classified along 10 different directions is developed and presented. The scheme is useful for understanding the auction building process, especially for those who approach the study of auctions. Promising future research directions on this subject are provided.
Alzheimer's disease and related dementias among Medicare beneficiaries aged ≥ 65 years in rural America, by Census region and select demographic characteristics: 2020
Authors: Matthews Kevin A. , Murray Christian T. , Nguyen Brenda L. , Spears Katie S. , Jackson Eva M. J. , Hall Diane M. , McGuire Lisa C. 
Published: January 2025

AbstractPurposeAlzheimer's disease and related dementias (ADRD) are a significant public health concern characterized by memory decline that, over time, leads to loss of independence. This study reports ADRD diagnosis rates among Medicare beneficiaries aged ≥ 65 years in rural America.MethodsWe conducted a descriptive analysis of Medicare Fee‐for‐Service (FFS) and Medicare Advantage enrollees using administrative Medicare data from 2020. Combining data from Medicare FFS and Medicare Advantage produces a more complete and representative sample of older adults than previous studies that used FFS data alone. Nonmetropolitan counties were used to define rural. Rural ADRD diagnosis rates, stratified by age, sex, race/ethnicity, and Census region, were adjusted using the 2000 Census population standard.FindingsThe study population consisted of 54 million Medicare data Fee‐for‐Service (FFS) and Medicare Advantage enrollees aged ≥ 65; 5.3 million beneficiaries were diagnosed with ADRD, and 16.2% (n = 861,337) of beneficiaries diagnosed with ADRD resided in rural America. The age‐adjusted ADRD diagnosis rate was slightly lower in rural America (9.6 per 100 beneficiaries) than in the United States (10.0 per 100 beneficiaries). The South Census region had the highest rural ADRD diagnosis rates.ConclusionThese findings underscore the need for targeted interventions and support mechanisms to address the growing burden of ADRD in rural communities.
Cotton RLP6 Interacts With NDR1/HIN6 to Enhance Verticillium Wilt Resistance via Altering ROS and SA
Authors: Zhang Dongmei , Wang Yan , Gu Qishen , Liu Lixia , Wang Zhicheng , Zhang Jin , Meng Chengsheng , Yang Jun , Zhang Zixu , Ma Zhiying , Wang Xingfen , Zhang Yan 
Published: January 2025

ABSTRACTCotton Verticillium wilt (VW) is often a destructive disease that results in significant fibre yield and quality losses in Gossypium hirsutum. Transferring the resistance trait of Gossypium barbadense to G. hirsutum is optional but challenging in traditional breeding due to limited molecular dissections of resistance genes. Here, we discovered a species‐diversified structural variation (SV) in the promoter of receptor‐like protein 6 (RLP6) that caused distinctly higher expression level of RLP6 in G. barbadense with the SV than G. hirsutum without the SV. Functional experiments showed that RLP6 is an important regulator in mediating VW resistance. Overexpressing RLP6 significantly enhanced resistance and root growth, whereas the opposite phenotype appeared in RLP6‐silenced cotton. A series of experiments indicated that RLP6 regulated reactive oxygen species (ROS) and salicylic acid (SA) signalling, which induced diversified defence‐related gene expression with pathogenesis‐related (PR) proteins and cell wall proteins enrichments for resistance improvement. These findings could be valuable for the transfer of the G. barbadense SV locus to improve G. hirsutum VW resistance in future crop disease resistance breeding.
Differential expression of CCD4(4B) drives natural variation in fruit carotenoid content in strawberry (Fragaria spp.)
Authors: Amaya Iraida , Roldán‐Guerra F. Javier , Ordóñez‐Díaz José L. , Torreblanca Rocío , Wagner Henning , Waurich Veronika , Olbricht Klaus , Moreno‐Rojas José M. , Sánchez‐Sevilla José F. , Castillejo Cristina 
Published: January 2025

SummaryCarotenoids are a diverse group of pigments imparting red, orange, and yellow hues to many horticultural plants, also enhancing their nutritional properties and health benefits. In strawberry, the genetic and molecular mechanisms regulating the natural variation of fruit carotenoid composition remain largely unexplored. In this study, we use a population segregating in yellow/white flesh to detect a major quantitative trait locus (QTL), qYellow Flesh‐4B, located on chromosome 4B and accounting for 82% of total phenotypic variation. In the QTL interval, specific polymorphisms on the promoter of the carotenoid cleavage dioxygenase CCD4(4B) were associated with yellow flesh, down‐regulation of CCD4(4B) during ripening, and increased carotenoid content. The role of CCD4(4B) in carotenoid turnover was further confirmed through transient overexpression in strawberry fruits, which resulted in decreased concentrations of the xanthophylls violaxanthin, lutein, and zeaxanthin. Notably, a −35 C>T single‐nucleotide polymorphism (SNP) in the CCD4(4B) promoter was predictive of both CCD4(4B) expression and carotenoid content across a diverse collection of octoploid Fragaria species. These findings provide valuable genetic insights into the natural variation of carotenoid composition and accumulation in strawberry. A high‐resolution melting (HRM) DNA test developed in this study offers a rapid and reliable method for predicting high carotenoid content in strawberry fruits, representing a valuable tool for breeding projects aimed at enhancing the nutritional value of this crop.
Chronic Kidney Disease‐Mineral Bone Disorder (CKD‐MBD) in Pediatric Kidney Transplant Recipients
Authors: Tamer Zenab , Hanudel Mark R. , Salusky Isidro B. 
Published: January 2025

ABSTRACTKidney transplantation remains the gold standard treatment for end‐stage kidney disease (ESKD), effectively alleviating numerous comorbidities and offering a substantial survival advantage over long‐term dialysis. Despite advancements in immunosuppressive regimens and improvements in graft and patient survival rates, extended patient longevity brings an accumulating burden and complexity of bone disease in this population, which often goes underrecognized. The present study reviews the pathophysiology of CKD‐MBD in pediatric KTR, focusing on the progression of bone disease before and after transplantation. We aim to enhance understanding of available screening options, highlighting their advantages and limitations, to support more informed decision‐making in CKD‐MBD management.
Transcatheter Pulmonary Flow Restrictors as a Palliative Bridge to Heart Transplant
Authors: Masilamani Mats Steffi Jennifer , Johnson Jonathan N. , Ameduri Rebecca K. , Stephens Elizabeth H. , Cabalka Allison K. , Anderson Jason H. 
Published: January 2025

ABSTRACTBackgroundIn recent years, transcatheter implantation devices to restrict pulmonary arterial flow have emerged as a potential alternative to surgical pulmonary artery banding.Case PresentationA term male was diagnosed with critical aortic stenosis (AS) and severely reduced left ventricle (LV) systolic function. He underwent aortic balloon valvuloplasty on day 2 of life, resulting in some antegrade flow, but LV ejection fraction only improved to 15%. He remained ductal dependent for systemic perfusion. Pulmonary over‐circulation ensued with systemic steal evident in his second week of life. On day 14, he underwent a transcatheter stage 1 procedure including the placement of bilateral pulmonary flow restrictor (PFR) devices and ductal stenting. Post‐procedure, he improved clinically, weaned off respiratory support, and transitioned to oral feeds while awaiting cardiac transplant.Twelve days later, he received a donor heart, with an uneventful recovery. He is now 3 years post‐transplant and has excellent graft function, and his transplant course has been unremarkable.ConclusionTranscatheter‐based palliation for a neonate with critical AS, utilizing endoluminal PFRs, proved effective in stabilizing the infant and successfully bridging him to transplant. This highlights an opportunity for the utilization of PFRs in neonatal conditions where control of pulmonary blood flow is imperative as a bridge to transplantation.
Abdominal fluid score as a predictor of transfusion needs and outcome in cats following trauma
Authors: Langlois Theresa J. , Mastrocco Alicia , Prittie Jennifer E. , Weltman Joel G. 
Published: January 2025

AbstractObjectiveTo assess the value of the abdominal fluid score (AFS) in cats following trauma in determining surgical needs, transfusion needs, and mortality.DesignMulticenter retrospective observational study utilizing data from the Veterinary Committee on Trauma (VetCOT) registry.SettingVetCOT Veterinary Trauma Centers.AnimalsA total of 2309 cats entered into the VetCOT registry between January 1, 2013, and December 31, 2020, were included.InterventionsNone.Measurements and Main ResultsOf the included cats, 148 had abdominal effusion on presentation (AFS 1–4, positive AFS [pAFS]). pAFS cats demonstrated a lower total plasma protein (6.2 g/dL, range: 2.8–9.2 g/dL vs 6.8 g/dL, range: 2.6–11 g/dL, P < 0.001) and a more negative base excess (BE) (−9, range: −23.7 to 10 vs −6.1, range: −31.7 to 1, P = 0.001) than cats without abdominal effusion (negative AFS [nAFS]). pAFS cats also had a higher baseline Animal Trauma Triage score (ATTS) than nAFS cats (5, range 0–12 vs 3, range 0–17, respectively, P < 0.001). pAFS cats were more likely to require blood transfusions (16% [23/148] vs 4% [94/2161], P < 0.001) than nAFS cats, but need for surgical intervention did not differ between groups. A pAFS was associated with increased need for hospitalization (62.8% [93/148] vs 47.7% [1030/2160], P < 0.001), but length of hospital stay did not differ. Cats with pAFS experienced higher mortality compared to nAFS cats (42.6% [63/148] vs 22.7% [491/2161], P < 0.001).ConclusionThe presence of abdominal effusion in cats following trauma was associated with increased morbidity in this study, as demonstrated by greater need for transfusion, increased likelihood of hospitalization, and greater mortality. Cats in the pAFS group had more negative BE and higher ATTS, but need for surgery, length of hospitalization, PCV, and blood glucose, plasma lactate, and ionized calcium concentrations were not different between groups.
Toward a Consistent Framework for Describing the Free Vibration Modes of the Brain
Authors: Jennings Turner , Amini Rouzbeh , Müftü Sinan 
Published: January 2025

Abstract
Frequency-domain analysis of brain tissue motion has received increased focus in recent years as an approach to describing the response of the brain to impact or vibration sources in the built environment. While researchers in many experimental and numerical studies have sought to identify natural resonant frequencies of the brain, sparse description of the associated vibration modes limits comparison of results between studies. We performed a modal analysis to extract the natural frequencies and associated mode shapes of a finite element (FE) model of the head. The vibration modes were characterized using two-dimensional (2D) plate deformation notation in the basic medical planes. Many of the vibration modes characterized are similar to those found in previous numerical and experimental studies. We propose this characterization method as an approach to increase compatibility of results between studies of brain vibration behavior.
Reduced-Order Modeling and Optimization of a Flapping-Wing Flight System
Authors: Reade J. , Cote B. , Jankauski M. 
Published: January 2025

Abstract
Flapping-wing micro-air vehicles (FWMAVs) are an emerging technology inspired by flying insects that show promise in applications favoring maneuverability and vehicle compactness. However, current designs are limited by inefficient energetics, and current dynamical models of the flight system employ limiting assumptions when considering power demands. Here, we derive a system-level model of the insect flight system including the thorax, wing, and wing hinge that can inform insect-inspired FWMAV design. We applied the model to study the flight system of a hawkmoth, and used a genetic algorithm optimization to tune uncertain model parameters to minimize the power required to hover. Results show that performance is improved by utilizing multimodal excitation to produce favorable flapping kinematics. This is achieved by locating the flapping frequency of the moth between the nonlinear resonant frequencies, resulting in magnified flapping response and aerodynamically advantageous phase. The optimal flapping frequency can be predicted from the system’s underlying linear natural frequencies and is roughly 54% of the system’s mean natural frequency. Furthermore, effective solutions are configured so that the timing of the applied load and thorax responses are matched such that little effort is spent reversing the wing stroke. The optimized model parameters and corresponding kinematics show moderate agreement with those reported for the hawkmoth. To maintain hovering flight, the successful moths in the population expend approximately 58.5 W/kg. The system-level model and the governing principles identified here can inform the design of energy efficient FWMAVs moving forward.
Master Curve Evaluation Using the Fracture Toughness Data at Low Temperature of T − T0 &lt; −50 °C
Authors: Yamamoto Masato , Kirk Mark , Shinko Tomoki 
Published: January 2025

Abstract
The Master Curve (MC) fracture toughness evaluation methodology standardized by ASTM E1921 and JEAC4216 specifies an acceptable test temperature, T, to be selected within the range of ±50 °C from the evaluated reference temperature, T0. If small size specimens, such as 4 mm thickness Mini-C(T), are used to measure fracture toughness, KJc, temperatures lower in this range should be selected to avoid an excessive number of KJc values being censored due to plastic strain evolution that leads to specimen failure above the KJclimit prescribed by both standards. The combined constraints imposed by the T0 ± 50 °C range and the KJclimit may cause difficulty in selecting an appropriate test temperature, especially for smaller specimens. The present study proposes a criterion based on Tave, the average of test temperature of uncensored valid KJc values in a dataset, to judge whether a dataset including data obtained T − T0 &lt; −50 °C can be used for estimation of T0. Multiple analyses with synthetic KJc datasets, which explore different minimum toughness and temperature dependence characteristics than the classical Master Curve assumption as sensitivity studies, demonstrated that if Tave − T0 ≧ −50 °C, the resultant T0 value predicts as Master Curve that reasonably represents the fracture toughness of the data even if the dataset includes some data tested at temperatures below T − T0 = −50 °C. Master Curve analyses performed on recursively sampled datasets from an experimental fracture toughness dataset of over 200 values demonstrated that the concept of Tave works reasonably well for T0 values determined from a realistic dataset size (e.g., 12–16). Allowing use of some data tested below T − T0 = −50 °C created a higher percentage of valid T0 evaluations than can be achieved using the existing requirements of either ASTM E1921 or JEAC4216.
Effects of vitamin B
12
supply on cellular processes of the facultative vitamin B
12
consumer
Vibrio campbellii
Authors: Groon Luna-Agrippina , Bruns Stefan , Dlugosch Leon , Wilkes Heinz , Wienhausen Gerrit 
Published: January 2025

ABSTRACT

Vitamin B
12
(cobalamin, herein B
12
) is a key cofactor for most organisms being involved in essential metabolic processes. In microbial communities, B
12
is often scarce, largely because only few prokaryotes can synthesize B
12
de novo
and are thus considered B
12
-prototrophs. B
12
-auxotrophy is mostly manifested by the absence of the B
12
-independent methionine synthase, MetE. Here, we focus on bacteria that we classified as facultative B
12
consumers as they encode both B
12
-independent and -dependent (MetH) methionine synthases yet largely cannot synthesize B
12
de novo
. The genus
Vibrio
belongs to this group, and our work shows that upon B
12
supply growth of
Vibrio campbellii
is accelerated and autoinducer-2 (AI-2) concentrations are enhanced. We speculate that methionine synthesis efficiency, dependent on B
12
availability, is linked to AI-2 synthesis. The precursor for AI-2 synthesis is S-adenosyl-L-homocysteine (SAH), which in turn requires methionine as a precursor. In almost all
Vibrio
species studied,
btuF
(B
12
-binding protein), which is required for B
12
uptake, and
cobD
(Adenosylcobinamide-phosphate synthase), which enables remodeling of B
12
-like compounds, are encoded on the same operon as
pfs
(or
mtnN
, Adenosylhomocysteine nucleosidase), the first enzyme in the two-step AI-2 synthesis reaction. Transcriptomic analyses show that virulence factors, such as toxin synthesis, fimbriae formation, and activation of the type-6 secretion system, which have been shown to be regulated by quorum sensing via AI-2, are significantly upregulated in
V. campbellii
when B
12
is available. Our results demonstrate that
V. campbellii
is a facultative B
12
consumer and indicate that B
12
availability affects AI-2 levels and thus potentially virulence factor regulation.

IMPORTANCE

Metabolites play a key role in microbial metabolism and communication. While vitamin B
12
is an essential cofactor for important enzymatic reactions, autoinducer-2 mediates interspecies signaling and can regulate the expression of genes that are crucial for virulence and survival. In our study, we hypothesize and present findings how these two important metabolites are linked in
Vibrio
species.
Vibrio campbellii
grows without B
12
but at an accelerated rate when B
12
is present, and we detect higher AI-2 values in cultures with B
12
amendment. Transcriptome analyses show how vitamin B
12
availability significantly upregulates gene expression of virulence factors such as toxin synthesis, fimbrial formation, and activation of the type-6 secretion system in
V. campbellii
.