bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myeloid-specific ablation of Basp1 ameliorates diet-induced NASH in mice by attenuating pro-inflammatory signaling
Authors: Meng Ziyi , Zhou Linkang , Hong Sungki , Qiu Xiaoxue , Chen Zhimin , Liu Tongyu , Inoki Ken , Lin Jiandie D. 
Published: July 2023

Background and Aims:
NASH represents a severe stage of fatty liver disease characterized by hepatocyte injury, inflammation, and liver fibrosis. Myeloid-derived innate immune cells, such as macrophages and dendritic cells, play an important role in host defense and disease pathogenesis. Despite this, the nature of transcriptomic reprogramming of myeloid cells in NASH liver and its contribution to disease progression remain incompletely defined.

Approach and Results:
In this study, we performed bulk and single-cell RNA sequencing (sc-RNA seq) analysis to delineate the landscape of macrophage and dendritic cell transcriptomes in healthy and NASH livers. Our analysis uncovered cell type-specific patterns of transcriptomic reprogramming on diet-induced NASH. We identified brain-abundant membrane-attached signal protein 1 (Basp1) as a myeloid-enriched gene that is markedly induced in mouse and human NASH liver. Myeloid-specific inactivation of Basp1 attenuates the severity of diet-induced NASH pathologies, as shown by reduced hepatocyte injury and liver fibrosis in mice. Mechanistically, cultured macrophages lacking Basp1 exhibited a diminished response to pro-inflammatory stimuli, impaired NLRP3 inflammasome activation, and reduced cytokine secretion.

Conclusions:
Together, these findings uncover Basp1 as a critical regulator of myeloid inflammatory signaling that underlies NASH pathogenesis.
Serum identification of at-risk MASH: The metabolomics-advanced steatohepatitis fibrosis score (MASEF)
Authors: Noureddin Mazen , Truong Emily , Mayo Rebeca , Martínez-Arranz Ibon , Mincholé Itziar , Banales Jesus M. , Arrese Marco , Cusi Kenneth , Arias-Loste María Teresa , Bruha Radan , Romero-Gómez Manuel , Iruzubieta Paula , Aller Rocio , Ampuero Javier , Calleja José Luis , Ibañez-Samaniego Luis , Aspichueta Patricia , Martín-Duce Antonio , Kushner Tatyana , Ortiz Pablo , Harrison Stephen A. , Anstee Quentin M. , Crespo Javier , Mato José M. , Sanyal Arun J. 
Published: July 2023

Background:Early identification of those with NAFLD activity score ≥ 4 and significant fibrosis (≥F2) or at-risk metabolic dysfunction-associated steatohepatitis (MASH) is a priority as these patients are at increased risk for disease progression and may benefit from therapies. We developed and validated a highly specific metabolomics-driven score to identify at-risk MASH.Methods:We included derivation (n = 790) and validation (n = 565) cohorts from international tertiary centers. Patients underwent laboratory assessment and liver biopsy for metabolic dysfunction-associated steatotic liver disease. Based on 12 lipids, body mass index, aspartate aminotransferase, and alanine aminotransferase, the MASEF score was developed to identify at-risk MASH and compared to the FibroScan-AST (FAST) score. We further compared the performance of a FIB-4 + MASEF algorithm to that of FIB-4 + liver stiffness measurements (LSM) by vibration-controlled transient elastography (VCTE).Results:The diagnostic performance of the MASEF score showed an area under the receiver-operating characteristic curve, sensitivity, specificity, and positive and negative predictive values of 0.76 (95% CI 0.72–0.79), 0.69, 0.74, 0.53, and 0.85 in the derivation cohort, and 0.79 (95% CI 0.75–0.83), 0.78, 0.65, 0.48, and 0.88 in the validation cohort, while FibroScan-AST performance in the validation cohort was 0.74 (95% CI 0.68–0.79;p= 0.064), 0.58, 0.79, 0.67, and 0.73, respectively. FIB-4+MASEF showed similar overall performance compared with FIB-4 + LSM by VCTE (p= 0.69) to identify at-risk MASH.Conclusion:MASEF is a promising diagnostic tool for the assessment of at-risk MASH. It could be used alternatively to LSM by VCTE in the algorithm that is currently recommended by several guidance publications.
PTPN9 dephosphorylates FGFR2pY656/657 through interaction with ACAP1 and ameliorates pemigatinib effect in cholangiocarcinoma
Authors: Zhao Liming , Liu Jialiang , Li Kangshuai , Zhang Chao , Chen Tianli , Liu Zengli , Tang Yongchang , Hu Xiaoqiang , Shi Anda , Shu Lizhuang , Huang Shaohui , Lian Shuo , Zhang Minghui , Li Hui , Sun Jinpeng , Yu Xiao , Zhang Zhongyin , Zhang Zongli , Xu Yunfei 
Published: July 2023

Abstract and Aim:
Cholangiocarcinoma (CCA) is a highly aggressive and lethal cancer that originates from the biliary epithelium. Systemic treatment options for CCA are currently limited, and the first targeted drug of CCA, pemigatinib, emerged in 2020 for CCA treatment by inhibiting FGFR2 phosphorylation. However, the regulatory mechanism of FGFR2 phosphorylation is not fully elucidated.

Approach and Results:
Here we screened the FGFR2-interacting proteins and showed that protein tyrosine phosphatase (PTP) N9 interacts with FGFR2 and negatively regulates FGFR2pY656/657. Using phosphatase activity assays and modeling the FGFR2-PTPN9 complex structure, we identified FGFR2pY656/657 as a substrate of PTPN9, and found that sec. 14p domain of PTPN9 interacts with FGFR2 through ACAP1 mediation. Coexpression of PTPN9 and ACAP1 indicates a favorable prognosis for CCA. In addition, we identified key amino acids and motifs involved in the sec. 14p-APCP1-FGFR2 interaction, including the “YRETRRKE” motif of sec. 14p, Y471 of PTPN9, as well as the PH and Arf-GAP domain of ACAP1. Moreover, we discovered that the FGFR2I654V substitution can decrease PTPN9-FGFR2 interaction and thereby reduce the effectiveness of pemigatinib treatment. Using a series of in vitro and in vivo experiments including patient-derived xenografts (PDX), we showed that PTPN9 synergistically enhances pemigatinib effectiveness and suppresses CCA proliferation, migration, and invasion by inhibiting FGFR2pY656/657.

Conclusions:
Our study identifies PTPN9 as a negative regulator of FGFR2 phosphorylation and a synergistic factor for pemigatinib treatment. The molecular mechanism, oncogenic function, and clinical significance of the PTPN9-ACAP1-FGFR2 complex are revealed, providing more evidence for CCA precision treatment.
Modified Basket Suture for a Floppy or Irregular Iris in Endothelial Keratoplasty
Authors: Ishikawa Sho , Sasaki Takanori , Maruyama Takahumi , Shinoda Kei 
Published: July 2023

Abstract:
Performing endothelial keratoplasty in an eye with a damaged or irregular iris can result in uneven air filling, air escape behind the pupil, anterior iris curvature, a shallow anterior chamber, an uneven anterior chamber depth, difficulty inserting and opening the graft, and iris trauma. We herein present a modified basket suture technique to prevent these iris complications. Before insertion of the corneal donor graft, a double-armed 9-0 polypropylene suture on a curved needle is inserted from 4 equidistant points at the corneal limbus to create a box configuration directly anterior to the iris. These sutures prevent the iris from moving toward the cornea and the anterior chamber from becoming shallow when air replacement is performed. The suture also reduces intraoperative or postoperative iris-related problems because the air filling is more uniform and less prone to migration.
Poor Sleep Quality and Working From Home Influence the Prevalence of Leisure Time Physical Inactivity During the COVID-19 Pandemic
Authors: Moura Samara Silva , Menezes Júnior Luiz Antônio Alves , Carraro Júlia Cristina Cardoso , Machado-Coelho George Luiz Lins , Meireles Adriana Lúcia 
Published: July 2023

Objective
The aim of the study is to explore possible associations according to quality of sleep and work from home (WFH) with physical inactivity (PI) during the COVID-19 pandemic.

Methods
This is a cross-sectional study in two Brazilian cities (>18 years), by probability sampling. Leisure-time physical activity was self-reported and classified according to guidelines. Sleep was by applying the Pittsburgh Sleep Quality Index. Work from home was classified as: total WFH or no WFH.

Results
A total of 1750 adults were analyzed, 69.1% were physically inactive, 51.9% had poor sleep quality, and 79.8% were not in WFH. PI was associated with poor sleep quality and non-WFH. Compared with those who reported WFH and good sleep quality, those who reported non-WFH and poor sleep quality had 4 times the prevalence of PI.

Conclusions
Poor sleep quality, and not WFH, is associated with leisure PI. These factors combined increase the probability of PI.
Effects of an Internet Delivered Behavioral Activation Program on Improving Work Engagement Among Japanese Workers
Authors: Dohi Yuri , Imamura Kotaro , Sasaki Natsu , Komase Yu , Sakuraya Asuka , Nakamura Yuri , Maejima Megumi , Aoyama Mitsuo , Kawakami Norito , Miyamoto Yuki 
Published: July 2023

Objective
The aim of the study is to examine the effect of a newly developed Internet-delivered behavioral activation (iBA) program on work engagement and well-being among Japanese workers with elevated psychological distress.

Methods
Participants were recruited via an Internet survey company (N = 3299). The eligibility criteria were as follows: (1) Japanese employees aged 20 to 59 years, (2) having psychological distress, and (3) not self-employed. This iBA program was a 3-week web-based training course using behavioral activation techniques. Work engagement, psychological distress, and eudemonic well-being at work were measured at baseline and postintervention period. A paired sample t test was conducted to assess the intervention effect.

Results
Of the 568 eligible participants, 120 were randomly selected. A total of 108 participants completed the baseline survey and received the iBA program. Eighty respondents completed the postintervention survey and were included in analyses. The iBA program did not show a significant intervention effect on work engagement (P = 0.22, Cohen d = 0.14), while psychological distress (P < 0.01, d = −0.40) and role-oriented future prospects (P = 0.02, Cohen d = 0.27) were significantly improved.

Conclusions
The effect of the iBA program on work engagement may be limited.
Perceived Workplace Health Support and Severe Psychological Distress Among Japanese Workers
Authors: Ikegami Kazunori , Ando Hajime , Kurogi Kazushirou , Ogami Akira 
Published: July 2023

Objective
This study aimed to assess the extent to which the intensity of perceived workplace health support (PWHS) affected the incidence of psychological distress.

Methods
At the baseline, data from 4540 eligible participants throughout Japan were obtained using an Internet survey. Among them, 2362 participants who could be followed up 1 year after the initial study were included in the analysis. The intensity of PWHS was determined using an original questionnaire. Psychological distress was assessed using the Kessler Psychological Distress Scale.

Results
Participants with high PWHS were less likely to experience severe psychological distress than those with low PWHS even after adjusting for confounding factors including work-related factors. The incidence of severe psychological distress decreased as the intensity of PWHS increased.

Conclusions
Enhanced PWHS reduced psychological distress and positively affected workers’ mental health.
Bacterial sexually transmitted infections are concentrated in subpopulations of MSM using HIV PrEP: an analysis of longitudinal trajectories
Authors: Jongen Vita W. , Loeff Maarten F. Schim Van Der , Van Den Elshout Mark , Wijstma Eline , Coyer Liza , Davidovich Udi , Vries Henry J.C. De , Prins Maria , Hoornenborg Elske , Boyd Anders 
Published: July 2023

Objective:
Studies have shown varying trends in incidence of sexually transmitted infections (STI) among individuals using HIV pre-exposure prophylaxis (PrEP). Characterization of individuals at increased risk for STIs may offer an opportunity for targeted STI screening.

Design:
Group-based trajectory modelling

Methods:
We screened participants from the AMPrEP demonstration project (2015–2020) for urogenital, anal, and pharyngeal chlamydia and gonorrhea, and syphilis every 3 months and when needed. We identified trajectories of STI incidence within individuals over time and determinants of belonging to a trajectory group. We calculated cumulative proportions of STIs within STI trajectory groups.

Results:
366 participants with baseline and ≥1 screening visit during follow-up were included (median follow-up time=3.7 years [IQR=3.5–3.7]). We identified three trajectories of STI incidence: (1) participants with a mean of approximately 0.1 STIs per 3-months (“low overall”, 52% of the population), (2) participants with a mean 0.4 STI per 3-months (“medium overall”, 43%), and (3) participants with high and fluctuating (between 0.3–1 STIs per 3-months) STI incidence (“high and fluctuating”, 5%). Participants in the “low overall” trajectory were significantly older, and reported less chemsex and condomless anal sex with casual partners than participants in the other trajectories. Participants in the “high and fluctuating” and “medium overall” groups accounted for respectively 23% and 64% of all STIs observed during follow-up.

Conclusions:
STI incidence was concentrated in subpopulations of PrEP users who were younger, had more chemsex and condomless anal sex. Screening frequency for STIs could be reduced for subpopulations with low risk for incident STIs.
Perfusion Computed Tomography in Rectal Carcinoma: Influence of Optimization of the Patlak Range on Calculation of Equivalent Blood Volume and Flow Extraction
Authors: Talakić Emina , Kaufmann-Bühler Ann-Katrin , Igrec Jasminka , Adelsmayr Gabriel , Janisch Michael , Döller Carmen , Geyer Edith , Lackner Karoline , Fuchsjäger Michael , Schöllnast Helmut 
Published: July 2023

Purpose
The aim of the study is to assess the influence of manual adjustment of the Patlak range in computed tomography (CT) perfusion analysis of rectal carcinoma compared with default range of the perfusion software.

Methods
This study was approved by the institutional review board and informed consent was obtained. Twenty-one patients (12 male, 9 female; mean age ± SD, 59 ± 11 years) with rectal cancer were included and underwent perfusion CT before preoperative chemoradiotherapy. Equivalent blood volume (BV) and flow-extraction (FE) were calculated using the Patlak plot model. Two perfusion sets were calculated per patient, a perfusion set using the default setting as provided by the software (dBV, dFE) and an optimized perfusion set after manual adaption of the Patlak range (aBV, aFE), which was limited to the intravascular space clearance of contrast to the extravascular space. Perfusion values calculated with both methods were compared for significance in differences using the Wilcoxon test. A P value of 0.05 or less was defined as statistically significant.

Results
Adjustment of the Patlak range statistically significantly influenced BV and FE calculation. Median dBV was 23.2 mL/100 mL (interquartile range [IQR], 12.1 mL/100 mL), whereas median aBV was 20.3 mL/100 mL (IQR, 10.9 mL/100 mL). The difference in BV was statistically significant (P = 0.021). Median dFE was 8.3 mL/min/100 mL (IQR, 4.7 mL/min/100 mL), whereas median aFE was 15.4 mL/min/100 mL (IQR, 5.8 mL/min/100 mL). The difference in FE was statistically significant (P < 0.001).

Conclusions
Our findings indicate that in perfusion CT of rectal carcinoma, adjustment of the Patlak range may significantly influence BV and FE compared with default setting of the software. This may contribute to standardization in the use of this technique for functional imaging of rectal cancer.
Patient and Provider Gender and Kidney Transplant Referral in Canada: A Survey of Canadian Healthcare Providers
Authors: Thanamayooran Aran , Foster Bethany J. , Tennankore Karthik K. , Vinson Amanda J. 
Published: July 2023

Background.
Referral for kidney transplant (KT) is variable, with women often disadvantaged. This study aimed to better characterize Canadian transplant referral practices and identify potential differences by respondent and/or patient gender using surveys targeted at healthcare practitioners (HCPs) involved in KT.

Methods.
Surveys consisting of 25 complex patient cases representing 7 themes were distributed to KT HCPs across Canada (March 3, 2022–April 27, 2022) using national nephrology/transplant society email registries. Respondents were asked whether they would refer the patient for transplant. Two identical surveys were created, differing only by gender/gender pronouns used in each case. Multivariable logistic regression was used to assess the association of respondent demographics and patient themes (including case gender) with the odds of transplant referral (overall and stratifying by respondent gender).

Results.
Overall, the referral rate was 58.0% among 97 survey respondents (46.4% male). Case themes associated with a lower likelihood of referral included adherence concerns (adjusted odds ratio [aOR] 0.65; 95% confidence interval [CI], 0.45-0.94), medical complexity (aOR 0.57; 95% CI, 0.38-0.85), and perceived frailty (aOR 0.63; 95% CI, 0.47-0.84). Respondent gender was not associated with differences in KT referral (aOR 0.91; 95% CI, 0.65-1.26 for male versus female respondents) but modified the association of frailty (less referral for male than female respondents, P = 0.005) and medical complexity (less referral for female than male respondents, P = 0.009) with referral. There were no differences in referral rate by case gender (P = 0.82).

Conclusions.
KT referral practices vary among Canadian HCPs. In this study, there were no differences in likelihood of transplant referral by candidate gender.
Large-scale cortical networks are organized in structured cycles
Authors: van Es Mats W.J. , Higgins Cameron , Gohil Chetan , Quinn Andrew J. , Vidaurre Diego , Woolrich Mark W. 
Published: July 2023

AbstractThe brain needs to perform a diverse set of cognitive functions essential for survival, but it is unknown how it is organized to ensure that each of these functions is fulfilled within a reasonable period. One way in which this requirement can be met is if each of these cognitive functions occur as part of a repeated cycle. Here, we studied the temporal evolution of large-scale cortical networks, and show that while network dynamics are stochastic, the overall ordering of their activity forms a robust cyclical pattern. This cyclical structure groups states with similar function and spectral content at specific phases of the cycle and occurs at timescales of (300-1000 ms). These results are reproduced in five large magnetoencephalography (MEG) datasets. Moreover, we show that metrics that characterize the cycle strength and speed are heritable, and relate to age, cognition, and behavioural performance. These results suggest that cortical network activations are inherently cyclical, ensuring periodic activation of essential cognitive functions.
Decoding working memory information from persistent and activity-silent neurons in the primate prefrontal cortex
Authors: Thrower Lilianna , Dang Wenhao , Jaffe Rye G. , Sun Jasmine D. , Constantinidis Christos 
Published: July 2023

ABSTRACTPersistent activity of neurons in the prefrontal cortex has been thought to represent the information maintained in working memory, though alternative models have recently challenged this idea. Activity-silent theories posit that stimulus information may be maintained by the activity pattern of neurons that do not produce firing rate significantly elevated about their baseline during the delay period of working memory tasks. We thus tested the ability of neurons that do and do not generate persistent activity in the prefrontal cortex of monkeys to represent spatial and object information in working memory. Neurons that generated persistent activity represented more information about the stimuli in both spatial and object working memory tasks. The amount of information that could be decoded from neural activity depended on the choice of decoder and parameters used but neurons with persistent activity outperformed non-persistent neurons consistently. Although averaged across all neurons and stimuli, firing rate did not appear clearly elevated above baseline during the maintenance of neural activity particularly for object working memory, this grant average masked neurons that generated persistent activity selective for their preferred stimuli, which carried the majority of information about the stimulus identity. These results reveal that prefrontal neurons with generate persistent activity constitute the primary mechanism of working memory maintenance in the cortex.NEW AND NOTEWORTHYCompeting theories suggest that neurons that generate persistent activity or do not are primarily responsible for the maintenance of information, particularly regarding object working memory. While the two models have been debated on theoretical terms, direct comparison of empirical results have been lacking. Analysis of neural activity in a large database of prefrontal recordings revealed that neurons that generate persistent activity were primarily responsible for the maintenance of both spatial and object working memory.
Taking time to compose thoughts with prefrontal schemata
Authors: Ryom Kwang Il , Basu Anindita , Stendardi Debora , Ciaramelli Elisa , Treves Alessandro 
Published: July 2023

AbstractUnder what conditions can prefrontal cortex direct the composition of brain states, to generate coherent streams of thoughts? Using a simplified Potts model of cortical dynamics, crudely differentiated into two halves, we show that once activity levels are regulated, so as to disambiguate a single temporal sequence, whether the contents of the sequence are mainly determined by the frontal or by the posterior half, or by neither, depends on statistical parameters that describe its microcircuits. The frontal cortex tends to lead if it has more local attractors, longer-lasting and stronger ones, in order of increasing importance. Its guidance is particularly effective to the extent that posterior cortices do not tend to transition from state to state on their own. The result may be related to prefrontal cortex enforcing its temporally-oriented schemata driving coherent sequences of brain states, unlike the atemporal “context” contributed by the hippocampus. Modelling a mild prefrontal (vs. posterior) lesion offers an account of mind-wandering and event construction deficits observed in prefrontal patients.