bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcription factor GLK1 promotes anthocyanin biosynthesis via an MBW complex‐dependent pathway in Arabidopsis thaliana
Authors: Li Yan , Lei Wei , Zhou Zuxu , Li Yanlin , Zhang Dawei , Lin Honghui 
Published: March 2023

ABSTRACTAnthocyanins are important natural plant pigments and play diverse roles in plant growth and adaptation. Anthocyanins function as screens to protect photosynthetic tissues from photoinhibition. However, the regulatory mechanisms underlying the biosynthesis and spatial accumulation pattern of anthocyanins remain some unresolved issues. Here, we demonstrate that the GARP‐type transcription factor GOLDEN2‐LIKE 1 (GLK1) functions as a positive factor in anthocyanin accumulation. GLK1 enhances the transcriptional activation activities of MYB75, MYB90, and MYB113 via direct protein‐protein interactions to increase the expression of anthocyanin‐specific biosynthetic genes. Anthocyanins accumulate in an acropetal manner in Arabidopsis. We also found that the expression pattern of GLK1 overall mimicked the accumulation pattern of anthocyanin from the base of the main stem to the shoot apex. Based on these findings, we established a working model for the role of GLK1 in anthocyanin accumulation and propose that GLK1 mediates the spatial distribution pattern of anthocyanins by affecting the transcriptional activation activities of MYB75, MYB90, and MYB113.
CPR5 positively regulates pattern‐triggered immunity via a mediator protein
Authors: Ma Miaomiao , Li Meng , Zhou Rongfang , Yu Jian‐Bin , Wu Ying , Zhang Xiaojuan , Wang Jinlong , Zhou Jian‐Min , Liang Xiangxiu 
Published: March 2023

SUMMARYPlant cells possess a two‐layered immune system consisting of pattern‐triggered immunity (PTI) and effector‐triggered immunity (ETI), mediated by cell surface pattern‐recognition receptors and intracellular nucleotide‐binding leucine‐rich repeat receptors (NLRs), respectively. The CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5) nuclear pore complex protein negatively regulates ETI, including ETI‐associated hypersensitive response. Here, we show that CPR5 is essential for the activation of various PTI responses in Arabidopsis, such as resistance to the non‐adapted bacterium Pseudomonas syringae pv. tomato DC3000 hrcC‐. In a forward‐genetic screen for suppressors of cpr5, we identified the mediator protein MED4. Mutation of MED4 in cpr5 greatly restored the defective PTI of cpr5. Our findings reveal that CPR5 plays opposite roles in regulating PTI and ETI, and genetically regulates PTI via MED4.
Neuroendocrine control of brown adipocyte function by prolactin and growth hormone
Authors: de Winne Catalina , Pascual Florencia L. , Lopez‐Vicchi Felicitas , Etcheverry‐Boneo Luz , Mendez‐Garcia Luis F. , Ornstein Ana Maria , Lacau‐Mengido Isabel Maria , Sorianello Eleonora , Becu‐Villalobos Damasia 
Published: March 2023

AbstractGrowth hormone (GH) is fundamental for growth and glucose homeostasis, and prolactin for optimal pregnancy and lactation outcome, but additionally, both hormones have multiple functions that include a strong impact on energetic metabolism. In this respect, prolactin and GH receptors have been found in brown, and white adipocytes, as well as in hypothalamic centers regulating thermogenesis. This review describes the neuroendocrine control of the function and plasticity of brown and beige adipocytes, with a special focus on prolactin and GH actions. Most evidence points to a negative association between high prolactin levels and the thermogenic capacity of BAT, except in early development. During lactation and pregnancy, prolactin may be a contributing factor that limits unneeded thermogenesis, downregulating BAT UCP1. Furthermore, animal models of high serum prolactin have low BAT UCP1 levels and whitening of the tissue, while lack of Prlr induces beiging in WAT depots. These actions may involve hypothalamic nuclei, particularly the DMN, POA and ARN, brain centers that participate in thermogenesis. Studies on GH regulation of BAT function present some controversies. Most mouse models with GH excess or deficiency point to an inhibitory role of GH on BAT function. Even so, a stimulatory role of GH on WAT beiging has also been described, in accordance with whole‐genome microarrays that demonstrate divergent response signatures of BAT and WAT genes to the loss of GH signaling. Understanding the physiology of BAT and WAT beiging may contribute to the ongoing efforts to curtail obesity.
Transnationalism: The fifth element of the Pacific Alliance's deep integration process
Authors: Prado Lallande Juan Pablo , Rouvinski Vladimir 
Published: March 2023

AbstractSince the governments of Chile, Colombia, Mexico, and Peru inaugurated a new Latin American integration mechanism, the Pacific Alliance, in 2011, the bloc has recorded both encouraging and disappointing results, depending on the evolution of the Alliance's main five aspects—political, economic, institutional, cooperative, and societal linkages. Although economic integration did not advance at the expected pace, owing to the abolition of a Mexican visa for Peru and Colombia, between 2011 and 2019, there has been a significant increase in social exchanges of nationals of the four countries, which included activities such as tourism, education, business trips, and others of similar nature (the Pacific Alliance's fifth element). Yet, despite these developments, scholars did not attempt to examine the societal linkage within the bloc, referred to in this article as the “fifth element” of the Pacific Alliance. To narrow the gap, this article examines the path of the Alliance toward deep integration by identifying the effect of strengthening social interactions, using a transnationalist framework and paying particular attention to the changing patterns of individual trips over more than 10 years of the Alliance's existence.
Climate lags and genetics determine phenology in quaking aspen (Populus tremuloides)
Authors: Blonder Benjamin W. , Brodrick Philip G. , Chadwick K. Dana , Carroll Erin , Cruz‐de Hoyos Roxanne M. , Expósito‐Alonso Moisés , Hateley Shannon , Moon Minkyu , Ray Courtenay A. , Tran Hoang , Walton James A. 
Published: March 2023

Summary
Spatiotemporal patterns of phenology may be affected by mosaics of environmental and genetic variation. Environmental drivers may have temporally lagged impacts, but patterns and mechanisms remain poorly known.
We combine multiple genomic, remotely sensed, and physically modeled datasets to determine the spatiotemporal patterns and drivers of canopy phenology in quaking aspen, a widespread clonal dioecious tree species with diploid and triploid cytotypes.
We show that over 391 km2 of southwestern Colorado: greenup date, greendown date, and growing season length vary by weeks and differ across sexes, cytotypes, and genotypes; phenology has high phenotypic plasticity and heritabilities of 31–61% (interquartile range); and snowmelt date, soil moisture, and air temperature predict phenology, at temporal lags of up to 3 yr.
Our study shows that lagged environmental effects are needed to explain phenological variation and that the effect of cytotype on phenology is obscured by its correlation with topography. Phenological patterns are consistent with responses to multiyear accumulation of carbon deficit or hydraulic damage.
Minority stress and intimate partner aggression among male same‐sex couples
Authors: Sarno Elissa L. , Smith Madison S. , Newcomb Michael E. 
Published: March 2023

AbstractIntimate partner aggression (IPA) is high among gay, bisexual, queer, and other sexual minority men (SMM), and is strongly linked to minority stress. These links might be further magnified or buffered by communication between partners (i.e., negative and positive communication, respectively). The present study investigated associations of minority stress and IPA, and the moderating role of positive/negative communication, among male couples (N = 932 individuals, 466 couples). Partners completed measures of communication skills, minority stress, and IPA, which were analyzed using moderated actor‐partner interdependence models. Results suggested that microaggressions increase one's own (i.e., an actor effect) and one's partner's (i.e., a partner effect) verbal IPA victimization, verbal IPA perpetration, and physical IPA victimization. Positive communication moderated the association between microaggressions and verbal IPA victimization, suggesting that high levels of positive communication may buffer the microaggression‐verbal IPA link. Thus, minority stress' detrimental impacts on relationship functioning among male same‐sex couples may be reduced by the presence of positive communication (e.g., effective conflict resolution). We discuss structural and clinical innovations to prevent IPA among male couples, with particular emphasis on the absence of positive communication as an aggravating factor.
Metabolic profiles in the xylem sap of Brassica juncea exposed to cadmium
Authors: Yang Zhen , Tan Piaopiao , Huang Zhihao , Sun Zhenzhen , Liu Zhixiang , Liu Lili , Zeng Chaozhen , Tong Jianhua , Yan Mingli 
Published: March 2023

AbstractMetabolic profiles in xylem sap are considered a fundamental mechanism for Cadmium (Cd) detoxification in plants. However, the metabolic mechanism of Brassica juncea xylem sap in response to Cd is still unclear. Here, we investigated the effects on the metabolomics of B. juncea xylem sap treated with Cd at different times by utilizing a nontargeted liquid chromatography‐mass spectrometry (LC‐MS)‐based metabolomics method for further elucidating the response mechanism of Cd exposure. The findings indicated that 48 h and 7 days Cd exposure caused significant differences in metabolic profiles of the B. juncea xylem sap. Those differential metabolites are primarily involved in amino acids, organic acids, lipids, and carbohydrates, and most of them were downregulated, which played essential roles in response to Cd stress. Furthermore, B. juncea xylem sap resisted 48‐h Cd exposure via regulation of glycerophospholipid metabolism, carbon metabolism, aminoacyl‐tRNA biosynthesis, glyoxylate and dicarboxylate metabolism, linoleic acid metabolism, C5‐branched dibasic acid metabolism, alpha‐linolenic acid metabolism, cyanoamino acid metabolism, ABC transporters, biosynthesis of amino acids, and pyrimidine metabolism; whereas alpha‐linolenic acid metabolism, glycerophospholipid metabolism, photosynthesis, and oxidative phosphorylation were regulated for resisting 7‐day Cd exposure.
Two Cases of Malingered Crime‐Related Amnesia
Authors: Zago Stefano , Preti Alice N. , Difonzo Teresa , D'Errico Annalisa , Sartori Giuseppe , Zangrossi Andrea , Bolognini Nadia 
Published: March 2023

AbstractAmnesia is a frequent claim in major crimes, and it is estimated that the complete or partial absence of memory following a crime ranges from 25% to 50% of total cases. Although some cases may constitute a genuine form of amnesia, due to organic‐neurological defects or psychological causes, and possibly combined with a dissociative or repressive coping style after an extreme experience, malingering is still fairly common in offenders. Therefore, one of the main goals in medico‐legal proceedings is to find methods to determine the credibility of crime‐related amnesia. At present, a number of lie and memory detection techniques can assist the forensic assessment of the reliability of declarative proof, and have been devised and improved over the past century: for example, modern polygraphs, event‐related potentials, thermal imaging, functional magnetic resonance imaging, kinematic, and facial analysis. Other ad hoc psychological tests, such as the so‐called Symptom Validity Test (SVT) and Performance Validity Test (PVT), as well as the autobiographical Implicit Association Test (aIAT), can also be used. To date, however, there is little evidence or case reports that document their real usefulness in forensic practice. Here, we report two cases of crime‐related amnesia, whereby both defendants, who were found guilty of homicide, appeared to exhibit dissociative amnesia but where the application of SVTs, PVTs, and aIAT detected a malingered amnesia.
ATM-SPARK: A GFP phase separation–based activity reporter of ATM
Authors: Li Xiaoquan , Chung Chan-I , Yang JunJiao , Chaudhuri Sibapriya , Munster Pamela N. , Shu Xiaokun 
Published: March 2023

The kinase ataxia telangiectasia mutated (ATM) plays a key role in the DNA damage response (DDR). It is thus essential to visualize spatiotemporal dynamics of ATM activity during DDR. Here, we designed a robust ATM activity reporter based on phosphorylation-inducible green fluorescent protein phase separation, dubbed ATM–SPARK (separation of phases-based activity reporter of kinase). Upon ATM activation, it undergoes phase separation via multivalent interactions, forming intensely bright droplets. The reporter visualizes spatiotemporal dynamics of endogenous ATM activity in living cells, and its signal is proportional to the amount of DNA damage. ATM-SPARK also enables high-throughput screening of biological and small-molecule regulators. We identified the protein phosphatase 4 that blocks ATM activity. We also identified BGT226 as a potent ATM inhibitor with a median inhibitory concentration of ~3.8 nanomolars. Furthermore, BGT226 sensitizes cancer cells to the radiomimetic drug neocarzinostatin, suggesting that BGT226 might be combined with radiotherapeutic treatment. ATM-SPARK achieves large dynamic range, bright fluorescence, and simple signal pattern.
Dating submarine landslides using the transient response of gas hydrate stability
Authors: Portnov Alexey , You Kehua , Flemings Peter B. , Cook Ann E. , Heidari Mahdi , Sawyer Derek E. , Bünz Stefan 
Published: March 2023

AbstractSubmarine landslides are prevalent on the modern-day seafloor, yet an elusive problem is constraining the timing of past slope failure. We present a novel age-dating technique based on perturbations to underlying gas hydrate stability caused by slide-impacted seafloor changes. Using three-dimensional (3-D) seismic data, we mapped an irregular bottom simulating reflection (BSR) underneath a submarine landslide in the Orca Basin, Gulf of Mexico. The irregular BSR mimics the pre-slide seafloor geometry rather than the modern bathymetry. Therefore, we suggest that the gas hydrate stability zone (GHSZ) is still adjusting to the post-slide sediment temperature. We applied transient conductive heat-flow modeling to constrain the response of the GHSZ to the slope failure, which yielded a most likely age of ca. 8 ka, demonstrating that gas hydrate can respond to landslides even on multimillennial time scales. We further provide a generalized analytical solution that can be used to remotely date submarine slides in the absence of traditional dating techniques.
Effect of covid-19 vaccination on long covid: systematic review
Authors: Byambasuren Oyungerel , Stehlik Paulina , Clark Justin , Alcorn Kylie , Glasziou Paul 
Published: March 2023

ObjectiveTo determine the effect of covid-19 vaccination, given before and after acute infection with the SARS-CoV-2 virus, or after a diagnosis of long covid, on the rates and symptoms of long covid.DesignSystematic review.Data sourcesPubMed, Embase, and Cochrane covid-19 trials, and Europe PubMed Central (Europe PMC) for preprints, from 1 January 2020 to 3 August 2022.Eligibility criteria for selecting studiesTrials, cohort studies, and case-control studies reporting on patients with long covid and symptoms of long covid, with vaccination before and after infection with the SARS-CoV-2 virus, or after a diagnosis of long covid. Risk of bias was assessed with the ROBINS-I tool.Results1645 articles were screened but no randomised controlled trials were found. 16 observational studies from five countries (USA, UK, France, Italy, and the Netherlands) were identified that reported on 614 392 patients. The most common symptoms of long covid that were studied were fatigue, cough, loss of sense of smell, shortness of breath, loss of taste, headache, muscle ache, difficulty sleeping, difficulty concentrating, worry or anxiety, and memory loss or confusion. 12 studies reported data on vaccination before infection with the SARS-CoV-2 virus, and 10 showed a significant reduction in the incidence of long covid: the odds ratio of developing long covid with one dose of vaccine ranged from 0.22 to 1.03; with two doses, odds ratios were 0.25-1; with three doses, 0.16; and with any dose, 0.48-1.01. Five studies reported on vaccination after infection, with odds ratios of 0.38-0.91. The high heterogeneity between studies precluded any meaningful meta-analysis. The studies failed to adjust for potential confounders, such as other protective behaviours and missing data, thus increasing the risk of bias and decreasing the certainty of evidence to low.ConclusionsCurrent studies suggest that covid-19 vaccines might have protective and therapeutic effects on long covid. More robust comparative observational studies and trials are needed, however, to clearly determine the effectiveness of vaccines in preventing and treating long covid.Protocol registrationOpen Science Frameworkhttps://osf.io/e8jdy.
Effect of Molybdenum Disulphide Thin Films on Enhancing the Performance of Polycrystalline Silicon Solar Cells
Authors: Rathanasamy Rajasekar , Kaliyannan Gobinath Velu , Sivaraj Santhosh , Muthiah Essakkiappan , Khaan Abdul Azeem Ajmal , Ravichandran Dharmaprakash , Uddin Md. Elias 
Published: March 2023

This research work focuses on augmenting the power conversion efficiency of the polycrystalline silicon solar cell with the aid of antireflection coating (ARC) of synthesized molybdenum disulphide (MoS2). The sol-gel technique and electrospraying method were preferred for synthesizing and depositing MoS2 as transparent thin films on the surface of the solar cells. The optical, electrical, structural, and thermal properties of the coated solar cells were analyzed for understanding the influence of the MoS2 coating. Five different samples (A-II, A-III, A-IV, A-V, and A-VI) were coated with varying coating time. Among them, 120 min coated sample experienced a maximum power conversion efficiency (PCE) of 17.96% and 18.82% under direct sunlight and neodymium light with resistivity as low as

2.79
×

10

−
3

Ω
−
cm

. The investigation of optical properties of the coated solar cells revealed a maximum transmittance of 93.6% and minimum reflectance of 6.3%, achieved for A-IV sample in the visible UV spectrum. Sample A-IV showed prominent results in the temperature analysis with temperatures as low as 38.9°C in uncontrolled and 43.2°C in controlled source environments. The results from various analyses proved that MoS2 was an appropriate material for an antireflection coating to enhance the performance of polycrystalline solar cell.
High-Throughput Peptide Arrays Identify Potential Diagnostic Autoantibody Signatures in Early-Stage Lung Adenocarcinoma
Authors: Luo Rongrong , Zhong Pei , Li Xiying , Cai Juan , Tao Yimin , Xiong Bangzhu , Zheng Hancheng , Zhang Zhishang , Tang Le , Yao Jiarui , Li Yingrui , Shi Yuankai , Han Xiaohong 
Published: March 2023

Abstract

Background:
Early diagnosis is critical to lung adenocarcinoma patients’ survival but faces inadequacies in convenient early detection.

Methods:
We applied a comprehensive microarray of 130,000 peptides to detect “autoantibody signature” that is autoantibodies binding to mimotopes for early detection of stage 0–I LUAD. Plasma samples were collected from 147 early-stage lung adenocarcinoma (Early-LUAD), 108 benign lung disease (BLD), and 122 normal healthy controls (NHC). Clinical characteristics, low-dose CT (LDCT), and laboratory tests were incorporated into correlation analysis.

Results:
We identified 143 and 133 autoantibody signatures, distinguishing Early-LUAD from NHC/BLD in the discovery cohort. Autoantibody signatures significantly correlated with age, stage, tumor size, basophil count, and IgM level (P &lt; 0.05). The random forest models based on differential autoantibody signatures displayed AUC of 0.92 and 0.87 to discern Early-LUAD from NHC/BLD in the validation cohort, respectively. Compared with LDCT, combining autoantibody signature and LDCT improved the positive predictive value from 50% to 78.33% (P = 0.049). In addition, autoantibody signatures displayed higher sensitivity of 72.4% to 81.0% compared with the combinational tumor markers (cyfra21.1, NSE, SCC, ProGRP) with a sensitivity of 22.4% (P = 0.000). Proteins matched by differential peptides were enriched in cancer-related PI3K/Akt, MAPK, and Wnt pathways. Overlaps between matched epitopes and autoantibody signatures illustrated the underlying engagement of autoantibodies in immune recognition.

Conclusions:
Collectively, autoantibody signatures identified by a high-throughput peptide microarray have the potential to detect Early-LUAD, which could assist LDCT to better diagnose Early-LUAD.

Impact:
Novel sensitive autoantibody signatures can adjuvant LDCT to better diagnose LUAD at very early stage.
Abstract CC1-1-1: To clip or not to clip (Part 1)
Authors: Caudle Abigail 
Published: March 2023

Abstract
Clinically node positive breast cancer patients often recieve neoadjuvant chemotherapy which can eradicate nodal disease. While sentinel node dissection alone has been shown to predict treatment response with a false negative rate of 12-14%, it is possible to increase the accuracy by performing targeted axillary dissection (TAD). To perform TAD, a clip is placed in a node if biopsy confirms disease. The procedure then involves selectively localizing and removing the clipped node in addition to the sentinel nodes. Multiple studies have show that this approach has a false negative rate of &lt;5%. These studies also show that the clipped node is not retrieved as a sentinel node in &gt; 20% of cases. This means that evaluating the sentinel nodes alone would miss evaluating the node with confirmed disease in a significant proportion of patients. This session will focus on the question of whether there is benefit to placing clips in nodes and ensuring removal after neoadjuvant chemotherapy.
Citation Format: Abigail Caudle. To clip or not to clip (Part 1) [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr CC1-1-1.
Abstract ED1-4: Patient Advocate Perspective
Authors: Spears Patty 
Published: March 2023

Abstract
Patient reported outcome (PRO) measures can be collected in clinical trials or clinical care through questionnaires. PROs allow a direct measure of how a patient is feeling and functioning, directly from the patient without interpretation by another person or clinician. PROs give patients a voice. Although there are challenges to collecting PROs, these challenges can be overcome. When deciding which measures to collect there are some things to keep in mind. For example, if the questions are relevant and meaningful to what the patient is experiencing they will be more likely to fill them out, providing useful information. The questionnaires can also be given in multiple formats (electronic, paper, telephone) to accommodate the preferences of different patients. However, collecting PROs is not enough, the information collected needs to be used to inform future patients or inform patient care. Additional challenges and benefits of collecting PROs in breast cancer research and clinical care from the patient perspective will be discussed.
Citation Format: Patty Spears. Patient Advocate Perspective [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr ED1-4.