bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of a prior eccentric lowering phase on concentric neuromechanics during multiple joint resistance exercise in older adults
Authors: Mc Dermott Emmet J. , Balshaw Thomas G. , Brooke‐Wavell Katherine , Maden‐Wilkinson Thomas M. , Folland Jonathan P. 
Published: June 2023

AbstractAging involves a marked decline in physical function and especially muscle power. Thus, optimal resistance exercise (RE) to improve muscle power is required for exercise prescription. An eccentric lowering phase immediately before a concentric lift (ECC‐CON) may augment concentric power production, due to various proposed mechanisms (e.g., elastic recoil, pre‐activation, stretch reflex, contractile history), when compared with a concentric contraction alone (CON‐Only). This study compared the effect of a prior eccentric lowering phase on older adult concentric power performance (ECC‐CON vs. CON‐Only) during a common multiple joint isoinertial RE (i.e., leg press) with a range of loads. Twelve healthy older adult males completed two measurement sessions, consisting of ECC‐CON and CON‐Only contractions, performed in a counterbalanced order using 20–80% of one repetition maximum [% 1RM] loads on an instrumented isoinertial leg press dynamometer that measured power, force, and velocity. Muscle activation was assessed with surface electromyography (sEMG). For mean power ECC‐CON>CON‐Only, with a pronounced effect of load on the augmentation of power by ECC‐CON (+19 to +55%, 35–80% 1RM, all p < 0.032). Similarly, for mean velocity ECC‐CON>CON‐Only, especially as load increased (+15 to 54%, 20–80% 1RM, all p < 0.005), but mean force showed more modest benefits of ECC‐CON (+9 to 14%, 50–80% 1RM, all p < 0.05). In contrast, peak power and velocity were similar for ECC‐CON and CON‐Only with all loads. Knee and hip extensor sEMG were similar for both types of contractions. In conclusion, ECC‐CON contractions produced greater power, and velocity performance in older adults than CON‐Only and may provide a superior stimulus for chronic power development.
The functional and structural characterization of Xanthomonas campestris pv. campestris core effector XopP revealed a new kinase activity
Authors: Kotsaridis Konstantinos , Michalopoulou Vassiliki A. , Tsakiri Dimitra , Kotsifaki Dina , Kefala Aikaterini , Kountourakis Nikos , Celie Patrick H. N. , Kokkinidis Michael , Sarris Panagiotis F. 
Published: June 2023

SUMMARYExo70B1 is a protein subunit of the exocyst complex with a crucial role in a variety of cell mechanisms, including immune responses against pathogens. The calcium‐dependent kinase 5 (CPK5) of Arabidopsis thaliana (hereafter Arabidopsis), phosphorylates AtExo70B1 upon functional disruption. We previously reported that, the Xanthomonas campestris pv. campestris effector XopP compromises AtExo70B1, while bypassing the host's hypersensitive response, in a way that is still unclear. Herein we designed an experimental approach, which includes biophysical, biochemical, and molecular assays and is based on structural and functional predictions, utilizing AplhaFold and DALI online servers, respectively, in order to characterize the in vivo XccXopP function. The interaction between AtExo70B1 and XccXopP was found very stable in high temperatures, while AtExo70B1 appeared to be phosphorylated at XccXopP‐expressing transgenic Arabidopsis. XccXopP revealed similarities with known mammalian kinases and phosphorylated AtExo70B1 at Ser107, Ser111, Ser248, Thr309, and Thr364. Moreover, XccXopP protected AtExo70B1 from AtCPK5 phosphorylation. Together these findings show that XccXopP is an effector, which not only functions as a novel serine/threonine kinase upon its host target AtExo70B1 but also protects the latter from the innate AtCPK5 phosphorylation, in order to bypass the host's immune responses. Data are available via ProteomeXchange with the identifier PXD041405.
Cholangiocytes express an isoform of soluble adenylyl cyclase that is N‐linked glycosylated and secreted in extracellular vesicles
Authors: Go Simei , Li Hang Lam , Chang Jung‐Chin , Verhoeven Arthur J. , Elferink Ronald P. J. Oude 
Published: June 2023

AbstractSoluble adenylyl cyclase (sAC)‐derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored. We consistently observed a 85 kD (sAC85) or 75 kD (sAC75) sAC protein band under glucose‐sufficient or glucose‐deprived states, respectively, in H69 cholangiocytes by immunoblotting. Deglycosylation by PNGase‐F demonstrated that both sAC75 and sAC85 are N‐linked glycosylated proteins with the same polypeptide backbone. Deglycosylation with Endo‐H further revealed that sAC75 and sAC85 carry distinct sugar chains. We observed release of N‐linked glycosylated sAC (sACEV) in extracellular vesicles under conditions that support intracellular sAC85 (glucose‐sufficient) as opposed to sAC75 (glucose‐deprived) conditions. Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles. The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h. Our observations support the maturation and trafficking in cholangiocytes of an N‐linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
Variations in the morphological phosphorus in a full‐scale A2O + MBR process with heavy chemical P removal addition dosing
Authors: Hu Yongfeng , Zhang Yongxiang , Wang Kun , Zhao Chonghui , Du Wei 
Published: June 2023

AbstractThe removal of biological phosphorus (P) removal along with chemical P removal additions is significantly important role in removing P at full‐scale wastewater treatment plants (WWTPs). Few research groups have recently reported the use of P forms in these WWTPs. The current study mainly presents the varied forms of morphological phosphorus and the fate of chemical P removal agents in a full‐scale WWTP that has adopted the A2O + MBR process. With time, the TP concentration in the liquid phase and the solid phase reaches the minimum (0.27 mg/L) and maximum values (1.75 mg/g), respectively. At this point, the TP concentration at the end of the aerobic and membrane pool becomes nearly constant. This indicates the complete removal of effluent TP from in the system. The heavy chemical P removal and addition dosing result in Al–P being the predominant P form in the solid phase. The fate of the chemical P removal and agents was determined by investigating the valences of the surface elements in freeze‐dried MLSS at the membrane pool.
Influence of Shaft Scratch on Static and Transient Behavior of Water-Lubricated Bearing
Authors: Liang Peng , Li Xingyang , Guo Feng , Zhang Xiaohan , Jiang Fulin 
Published: June 2023

Abstract
When sand enters the gap between the shaft and water-lubricated bearing, it will nick surfaces of shaft and bearing bush, and the scratch will appear. The variations of static and transient performance with the number and depth of scratches are studied in the paper. The results show that scratches have a significant effect on the critical load and critical speed of the transformation of bearing lubrication state. The existence of scratches reduces the critical load from elastohydrodynamic lubrication to mixed lubrication. The shaft with scratches vibrates more strongly than the shaft with no scratch at the moment of start-up. The contact area, contact time, and bush-burning probability are directly proportional to the number of scratches. Shaft center movement orbits under step load have the similar “L” shape, whether the shaft has scratch or not. But the scratched shaft has longer movement orbit and lower equilibrium point than the shaft with no scratch. This paper can provide a reference for structure design and service life evaluation of bearing-shaft system.
Performance Investigation of a Solar Air Heater Artificially Roughened With Joukowski Airfoil Ribs
Authors: Roy Bibhrat , Reddy Desireddy Shashidhar , Khan Mohd. Kaleem 
Published: June 2023

Abstract
This paper investigates a solar air heater’s thermohydraulic and thermogeometric performances with an artificially roughened absorber plate with Joukowski airfoil ribs. The rib height and shape have a bearing on the overall performance of the air heater. Joukowski airfoil ribs of different sizes are generated from a circular rib of a radius of 1 mm using conformal mapping. Simulations are performed for the turbulent flow of air through the roughened duct in the Reynolds number (Re) range of 4000 ≤ Re ≤ 15,000 using ansys fluent version 2020. The renormalization group kinetic energy-turbulence dissipation rate (RNGκ−ε) model with enhanced wall treatment (EWT) has been employed to model the turbulent flow. The grid refinement study is performed to optimize the mesh size and estimate the numerical solution error. The proposed rib design is tested for both headwind and tailwind flow arrangements. The tailwind performance is better for smaller-size Joukowski ribs. However, the medium- and large-size Joukowski ribs perform better in headwind configurations. At low Re, heat transfer is more dominant than friction leading to higher thermohydraulic performance, whereas, at high Re, the reverse trend is observed.
Investigation on Flame and Spray Characteristics of Butanol and Lemon Peel Oil Blends With Gasoline Using Optical Engine
Authors: Charlapally Shashank Sharma , Biswal Yogesh , Nayak Guguloth Mahesh , Chinnadurai Karthick , Balusamy Saravanan , Kasianantham Nanthagopal 
Published: June 2023

Abstract
The current research investigates the spray behavior of lemon peel oil (LPO) and butanol in a controlled environment under various engine-like conditions. The liquid spray morphology of both fuel blends is captured using a standard Mie scattering technique, and the liquid spray penetration length is compared to a baseline fuel isooctane. In order to simulate and create engine-like conditions, these experiments are carried out in a constant volume chamber under various pressure and temperature conditions. Furthermore, the combustion quality of binary and ternary blends is studied using an optical gasoline direct injection engine at three different injection timings. According to the constant volume spray study, isooctane has the shortest penetration. Because of its higher boiling point, LPO has a longer liquid spray penetration length. Despite its lower boiling point, butanol penetrates better than isooctane. The temperature was also discovered to influence liquid spray tip penetration length more than pressure significantly. In-cylinder combustion imaging results also revealed that injection timing significantly impacts combustion. Although butanol improves combustion, LPO-dominant blends demonstrated more diffusion burning due to poor evaporation characteristics. The blends prepared for the study were similar to gasoline in combustion conditions. It was discovered that these blends ran optimally without requiring any modifications to existing engines, even though late injection is recommended to improve combustion quality and peak performance.
Swimming With the COSMED AquaTrainer and K5 Wearable Metabolic System in Breath-by-Breath Mode: Accuracy, Precision, and Repeatability
Authors: Zacca Rodrigo , Castro Flávio Antônio de Souza , Monteiro Ana Sofia Mottini , Pyne David B. , Vilas-Boas João Paulo , Fernandes Ricardo Jorge Pinto 
Published: June 2023

Purpose: To compare ventilatory and cardiorespiratory responses between the COSMED AquaTrainer coupled with the K4b2 and K5 wearable metabolic systems in breath-by-breath mode over a wide range of swimming speeds. Methods: Seventeen well-trained master swimmers performed 2 front-crawl 7 × 200-m incremental intermittent protocols (increments of 0.05 m·s−1 and 30-s rest intervals, with a visual pacer) with AquaTrainer coupled with either K4b2 or K5. Results: Post hoc tests showed that swimming speed was similar (mean diff.: −0.01 to 0.01 m·s−1; P = .73–.97), repeatable (intraclass correlation coefficient: .88–.99; P < .001), highly accurate, and precise (agreement; bias: −0.01 to 0.01 m·s−1; limits: −0.1 to 0.1 m·s−1) between all conditions. Ventilatory and cardiorespiratory responses were highly comparable between all conditions, despite a “small” effect size for fraction of expired carbon dioxide at the sixth 200-m step (0.5%; ; P = .04) and carbon dioxide production at the fifth, sixth, and seventh 200-m steps (0.3–0.5 L·min−1; –.17; P = .01–.05). We also observed high accuracy, which was greater for tidal volume (0.0–0.1 L), minute ventilation (−3.7 to 5.1 L·min−1), respiratory frequency (bias: −2.1 to 1.9 breaths·min−1), and oxygen uptake (0.0–0.2 L·min−1). Bland–Altman plots showed that the distribution inside the limits of agreement and their respective 95% CIs were consistent for all ventilatory and cardiorespiratory data. The repeatability (intraclass correlation coefficient) of tidal volume (.93–.97), minute ventilation (.82–.97), respiratory frequency (.68–.96), fraction of expired carbon dioxide (.85–.95), carbon dioxide production (.77–.95), fraction of expired oxygen (.78–.92), and oxygen uptake (.94–.98) data ranged from moderate to excellent (P < .001–.05). Conclusions: Swimming with the AquaTrainer coupled with K5 (breath-by-breath mode) yields accurate, precise, and repeatable ventilatory and cardiorespiratory responses when compared with K4b2 (previous gold standard). Swimming support staff, exercise and health professionals, and researchers can now relate differences between physiological capacities measured with the AquaTrainer while coupled with either of these 2 devices.
Exercise and Health Anxiety in Older Women: Exploring the Mediating Role of Anxiety Sensitivity
Authors: Nah Kyoungsil , Olthuis Janine V. 
Published: June 2023

Research shows that health anxiety significantly affects older adults’ health and quality of life. Although exercise may be associated with lower health anxiety, research on older adults is limited, and the mechanism remains unknown. This study examined the association between exercise and health anxiety in older women and the mediating role of anxiety sensitivity in this association. Participants were 166 women aged 65+ years, without health problems that prevented them from exercising. Participants were recruited via Facebook advertisements. They completed an online self-report questionnaire including measures of health anxiety, physical activity, and anxiety sensitivity. The SPSS PROCESS macro was used to examine the association between exercise and health anxiety, and the role of anxiety sensitivity as a mediator. Results indicated that greater participation in exercise was associated with lower health anxiety, and anxiety sensitivity mediated the association. Findings suggest that exercise-based interventions might prevent health anxiety in older adults.
Intravascular ultrasound characteristics of different types of stenosis in idiopathic intracranial hypertension with venous sinus stenosis
Authors: Raynald  , Chen Na , Yang Hongchao , Tong Xu , Sui Binbin , Qu Hui , Dong Kehui , Wang Yilong , Miao Zhongrong , Mo Dapeng 
Published: June 2023

BackgroundIn this study, we analyzed the characteristics of different stenosis types in idiopathic intracranial hypertension (IIH) patients with venous sinus stenosis (VSS) using intravascular ultrasound (IVUS).MethodsWe retrospectively reviewed data from patients who underwent IVUS evaluation during venography or stenting procedures between January 2014 and February 2022.ResultsAmong the 80 patients with intrinsic lesions, 47 cases were identified, including 41 single lesions and 6 multiple lesions. Single lesions consisted of 36 cases of AG, 3 cases of brain herniation, and 2 cases of septation. Multiple intrinsic lesions were found in 6 patients, with AG observed in the transverse sinus and sigmoid sinus. IVUS features varied depending on the anatomical variations of intrinsic stenosis. Additionally, among the 33 cases of extrinsic stenosis, two types were observed: unilateral compression (22 cases) and bilateral compression (11 cases), primarily affecting the transverse sinus.ConclusionIVUS effectively differentiated intrinsic and extrinsic types of stenosis and identified intraluminal and mural components of intrinsic stenosis.
Dural arteriovenous fistula onyx embolization through a non-visible previously embolized pedicle
Authors: Colasurdo Marco , Chen Huanwen , Khunte Mihir , Malhotra Ajay , Gandhi Dheeraj 
Published: June 2023

Cerebral dural arteriovenous fistulas (dAVFs) are commonly treated with endovascular embolization, either through arterial or venous routes in single or multi-staged procedures. Treatment outcomes depend on multiple factors, including the patient’s clinical status, the anatomy of the malformation, and the operator’s experience, but technical success is also highly dependent on choice of neurovascular devices and embolic agents. When transvenous routes are not feasible and the most obvious transarterial routes do not appear safe, deep knowledge of the dynamic fluid properties of liquid embolics can be a valuable asset. We present a case(video 1) in which a complex skull-base dAVF was completely occluded through a non-visualized previously partially embolized arterial feeder. It is a unique case in which the embolization takes advantage of the peculiar fluid dynamic properties of non-adhesive embolic agents (Onyx-Medtronic, Minnesota, USA) 18 and 34.1–3Video 1 Technical video presenting a dAVF completely cured through a non-visible previously embolized arterial pedicle.
Anticitrullinated peptide antibody epitope expansion and the HLA DRB1 ‘shared epitope’ are less common in seropositive checkpoint inhibitor-induced inflammatory arthritis than in longstanding rheumatoid arthritis
Authors: Ghosh Nilasha , Reid Pankti , Aude Carlos Andres , Kirschman Jessica , Goodman Susan , Bykerk Vivian P , Lakhanpal Amit , Rajesh Diviya , Chan Karmela K , Robinson William H , Bass Anne R 
Published: June 2023

BackgroundImmune checkpoint inhibitors (ICI) can potentially cause ICI-inflammatory arthritis (ICI-IA), which often resembles rheumatoid arthritis (RA). In this study, we examined the degree of anticitrullinated peptide antibodies (ACPA) epitope expansion in CCP+ICI-IA and patients with RA.MethodsWe used clinical data and serum from ICI-IA and patients with RA with early disease as well as longstanding disease. A custom, bead-based antigen array was used to identify IgG ACPA reactivities to 18 putative RA-associated citrullinated proteins. Hierarchical clustering software was used to create a heatmap to identify ACPA levels. Additionally, HLA DRB1 typing was performed on ICI-IA patients as well as controls of patients treated with ICI that did not develop ICI-IA (ICI controls).ResultsCompared to patients with CCP+RA, patients with CCP+ICI-IA were older (p<0.001), less likely to have positive rheumatoid factor (p<0.001) and had a shorter duration of symptoms (p<0.001). There were less ACPA levels and a lower number of distinct ACPA epitopes in the serum of patients with ICI-IA compared with longstanding patients with RA (p<0.001). Among those tested for HLA DRB1, there were no differences in the frequency of the shared epitope between those with ICI-IA and ICI controls.ConclusionPatients with ICI-IA had lower ACPA titres and targeted fewer ACPA epitopes than longstanding patients with RA, and there were no significant differences in the presence of the shared epitope between those that developed ICI-IA and ICI controls. It remains to be determined if ICI-IA represents an accelerated model of RA pathogenesis with ICI triggering a transition from preclinical to clinical disease.
Biomechanical Evaluation of Posterior Shoulder Instability With a Clinically Relevant Posterior Glenoid Bone Loss Model
Authors: Waltz Robert A. , Brown Justin , Brady Alex W. , Bartolomei Christopher , Dornan Grant J. , Miles Jon W. , Arner Justin W. , Millett Peter J. , Provencher Matthew T. 
Published: June 2023

Background: Existing biomechanical studies of posterior glenoid bone loss and labral pathology are limited by their use of anterior instability models, which differ in both orientation and morphology and have been performed in only a single, neutral arm position. Purpose: To evaluate the biomechanical effectiveness of a posterior labral repair in the setting of a clinically relevant posterior bone loss model in various at-risk arm positions. Study Design: Controlled laboratory study. Methods: Ten fresh-frozen cadaveric shoulders were tested in 7 consecutive states using a 6 degrees of freedom robotic arm: (1) native, (2) posterior labral tear (6-9 o’clock), (3) posterior labral repair, (4) mean posterior glenoid bone loss (7%) with labral tear, (5) mean posterior glenoid bone loss with labral repair, (6) large posterior glenoid bone loss (28%) with labral tear, and (7) large posterior glenoid bone loss with labral repair. Bone loss was created using 3-dimensional printed computed tomography model templates. Biomechanical testing consisted of 75 N of posterior-inferior force and 75 N of compression at 60° and 90° of flexion and scaption. Posterior-inferior translation, lateral translation, and peak dislocation force were measured for each condition. Results: Labral repair significantly increased dislocation force independent of bone loss state between 10.1 and 14.8 N depending on arm position. Dislocation force significantly decreased between no bone loss and small bone loss (11.9-13.5 N), small bone loss and large bone loss (9.4-14.3 N), and no bone loss and large bone loss (21.2-26.5 N). Labral repair significantly decreased posterior-inferior translation compared with labral tear states by a range of 1.0 to 2.3 mm. In the native state, the shoulder was most unstable in 60° of scaption, with 29.9 ± 6.1-mm posterior-inferior translation. Conclusion: Posterior labral repair improved stability of the glenohumeral joint, and even in smaller to medium amounts of posterior glenoid bone loss the glenohumeral stability was maintained with labral repair in this cadaveric model. However, a labral repair with large bone loss could not improve stability to the native state. Clinical Relevance: This study shows that larger amounts of posterior glenoid bone loss (>25%) may require bony augmentation for adequate stability.
Exploring the Impact of the COVID-19 Pandemic on Employees’ Workplace Health Promotion Preferences
Authors: Hammerback Kristen , Strait Michelle , Kohn Marlana J. , Garcia Cinthya , Harris Jeffrey R. , Hannon Peggy A. 
Published: June 2023

Purpose The COVID-19 pandemic has led to profound changes in the workplace as well as increases in stress, missed preventive care, and other health concerns. There is limited research since the onset of the pandemic on employees’ primary health concerns and their willingness to engage with workplace health promotion (WHP) programs to address these needs. We conducted this survey about employees’ current health priorities as a first step to exploring whether WHP programs need to evolve to be responsive to employees’ needs at this stage of the pandemic. Design National cross-sectional survey. Setting United States, April 29-May 5, 2022. Subjects 2053 Americans employed part or full time. Measures 17-item online survey assessing demographics, health priorities, and impact of the pandemic on health. Analysis Descriptive statistics, SPSS Version 19. Results Employees’ most common health concerns included work/life balance and stress (each cited by 55%). Nearly half (46%) said their health or well-being was affected by the pandemic; within this group, the most common concerns were stress (66%), anxiety (61%), sleep (49%), and depression (48%). Almost all (94%) indicated they would be open to receiving support from their employers. Conclusion This research is a first step in learning about employees’ current health priorities and how they may have changed. WHP researchers and practitioners can determine how their programs align with current priorities. Our future research will explore employees’ preferences, heath behaviors, and their current workplace environments in more depth.