bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of thermally deteriorated oil re-used as adulterants in sunflower oil using Fourier transform infrared spectroscopy
Authors: Bunaciu Andrei A. , Hoang Vu Dang , ABOUL-ENEIN Hassan 
Published: January 2023

Abstract
Sunflower oil (SFO) is preferably chosen for frying because it gets soaked less and is very light. Nevertheless, there is a possibility that SFO is adulterated with thermally deteriorated oil re-used such as the leftover oil after the frying process of meat with breadcrumbs (TDO A) and potatoes (TDO B). It may be very harmful to our health and the environment, requiring a fast and reliable screening test for such adulterants. In this study, Fourier transform infrared spectroscopy based on the attenuated total reflectance (ATR-FITR) sampling technique was studied for the direct determination of TDOA and TDO B as adulterants in SFO-TDO binary mixtures. Our proposed quantification methods were based on the simple linear regression analysis of IR spectral intensity (peak height or peak area) at the wavenumber 1746 cm − 1 on adulterant concentration 0 ÷ 10% (v/v) (R2 > 0.990). They proved to be accurate (% recovery in the range of 98.0 ÷ 101.1%), precise (RSD < 2.7%) and technically simple (total analysis time of 5–10 min).
Adaptive Force of hamstring muscles is reduced in patients with knee osteoarthritis compared to asymptomatic controls
Authors: Schaefer Laura V , Dech Silas , Carnarius Friederike , Rönnert Florian , Bittmann Frank N , Becker Roland 
Published: January 2023

Abstract
Quadriceps strength deficits are known for patients with knee osteoarthritis (OA). Hamstring muscles has been less often investigated and findings are not as clear as for knee extensors. In regard of OA, the neuromuscular function in the sense of Adaptive Force (AF) has never been examined before. The maximal isometric AF (AFisomax) was previously considered to be especially vulnerable to disruptive stimuli. The present study investigated AF parameters and the maximal voluntary isometric contraction (MVIC) of hamstring muscles bilaterally of 20 knee OA patients (ART) compared to 19 asymptomatic controls (CON). For each side, MVIC was captured before and after AF measurements. Five AF trials were performed using a pneumatically driven device. Participants should maintain an isometric position despite an increasing load of the device. After reaching AFisomax, the muscle merged into eccentric muscle action whereby the force increased further to the maximal AF (AFmax). All torque parameters (normalized to body mass) were significantly lower in ART vs. CON for both sides with mostly very large effect sizes. MVIC deficits amounted 28% for the more and 30% for the less affected/asymptomatic side. AFmax showed deficits of 36% and 32% and AFisomax of 37% and 24%, respectively. The reduction of AFisomax was not as high as expected, especially compared to MVIC or AFmax. The ratio AFmax to MVIC was significantly lower for the more affected side of ART (84 ± 17%) vs. CON (95 ± 7%). This indicates that OA patients could fetch less of their maximal strength during eccentric action compared to controls. The not confirmed hypothesized stronger deficits in maximal holding capacity (AFisomax) could result from the seating measurement position, in which a knee strain relief was present. This might have reduced inhibitory nociceptive effects which were expected to lead to even higher deficits for the holding capacity. The results suggest that general hamstrings strength deficits are present in ART vs. CON, which might be due to nociceptive inhibitory effects or to a generally lower load of lower extremity due to higher inactivity of OA patients.
De novo transcriptome analysis and identification of defensive genes in garlic (Allium sativum L.) using high-throughput sequencing
Authors: Prajapati Malyaj R , Singh Jitender , Kumar Pankaj , Dixit Rekha 
Published: January 2023

Abstract
Background: Garlic (Allium sativum L.) is the second most widely cultivated Allium which is mainly grown in temperate regions and used as a flavouring agent in wide variety of foods. Garlic contains various bioactive compounds whose metabolic pathways, plant pathogen interactions, defensive genes, identify interaction networks and functional genomics was not previously predicted in the garlic at genomic level. To address this issue, we constructed two garlic Illumina 2000 libraries from tissues of garlic clove and leaf.
Results: Approximately 43 million 125 bp paired-end reads were obtained in the two libraries. A total of 239,973 contigs were generated by de novo assembly of both samples and were compared with the sequences in NCBI non-redundant protein database. In total, 42% of contigs were matched to known proteins in public databases including Nr, GO and COG and then contigs were mapped to 138 via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). In addition, a number of regulatory genes including CCHC(Zn) family, followed by WD40, bromodomain, bZIP, AP2-EREBP, BED-type (Zn) proteins and defense response proteins related different conserved domains, such as RGA3, NBS-LRR, TIR-NBS-LRR, LRR, NBS-ARC, and CC-NBS-LRR. were discovered based on transcriptome dataset. We compared the ortholog gene family of the A. sativum transcriptome to A. thaliana, O. sativa and Z. mays and found that 12,077 orthologous gene families are specific to A. sativum L. Furthermore, we identified genes involved in plant defense mechanism, their protein-protein interaction network and plant pathogen interaction pathways.
Conclusions: Our study contains an extensive sequencing and functional gene-annotation analysis of A. sativum L. The findings provide insights into molecular basis of TFs, defensive gene and a reference for future studies on the genetics and breeding of A. sativum L.
Establishment and inheritance of minichromosomes from Arabidopsis haploid induction
Authors: Tan Ek Han , Ordoñez Benny , Thondehaalmath Tejas , Seymour Danelle , Maloof Julin , Maruthachalam Ravi , Comai Luca 
Published: January 2023

Abstract
Minichromosomes are small, sometimes circular, rearranged chromosomes consisting of one centromere and short chromosomal arms, formed by treatments that break DNA, including plant transformation. Minichromosomes have the potential to serve as vectors to quickly move valuable genes across a wide range of germplasm, including into adapted crop varieties. To realize this potential, minichromosomes must be reliably generated, easily manipulated, and stably inherited. Here we show a reliable method for minichromosome formation in haploids resulting from CENH3-mediated genome elimination, a process that generates genome instability and karyotypic novelty specifically on one parental genome. First, we identified 2 out of 260 haploids each containing a single-copy, minichromosome originating from centromeric regions of chromosome 1 and 3 respectively. The chromosome 1 minichromosome we characterized did not pair at meiosis, but displayed consistent transmission over nine selfing generations. Next, we demonstrated that CENH3-based haploid induction can produce minichromosomes in a targeted manner. Haploid inducers carrying a selectable, pericentromeric marker were used to isolate additional chromosome-specific minichromosomes which occurred in 3 out of 163 haploids. Our findings document the formation of heritable, rearranged chromosomes and we provide a method for convenient minichromosome production.
Magmatic degassing controlled the metal budget of the Axi epithermal gold deposit, China
Authors: Li Nuo , Zhang Bo , Ulrich Thomas , Williams-Jones A.E. , Chen Yanjing 
Published: January 2023

Abstract
From integrated textural and compositional studies of auriferous and barren pyrite/marcasite in the epithermal Axi gold deposit, China, we have identified a relationship between multiple gold mineralizing events, mafic magma recharge, and fluid-rock reactions. Three generations of pyrite (Py1–3) and four generations of marcasite (Mar1–4) record episodic gold mineralizing events, followed by silver-copper-lead-zinc-cadmium enrichment. The gold mineralizing events are recorded by high concentrations of subnanometer-sized gold in Py1, Py3, and Mar3 (max. = 147, 129, and 34 ppm, med. = 39, 34, and 12 ppm). Based on previous Re-Os age determinations of pyrite and U-Pb zircon ages of the andesitic wallrock, these gold events slightly postdate pulsed mafic magma recharge and represent the incursion of Au-As-S-rich magmatic volatiles into circulating meteoric water. Silver-Cu-Pb-Zn-Cd enrichment in Py2, Mar2, and Mar4 are consistent with quiescent degassing and gradual Ag-Cu-Pb-Zn-Cd enrichment in an evolved felsic magma. Barren Mar1 records the dominance of meteoric water and a limited magmatic fluid contribution. High-Co-Ni-V-Cr-Ti contents in porous cores of Py1 and Mar2 are attributed to wall rock alteration and dissolution-reprecipitation. The results provide convincing evidence that the metal budget (especially for Au, Ag, Cu, Pb, Zn, Sb) of the hydrothermal fluids and sulfides in epithermal systems are controlled by the influx of magmatic fluids and associated magma, whereas the enrichment of certain fluid-immobile elements, such as Co, Ni, V, Cr, and Ti, is caused in part by fluid-rock interaction.
Long-term hydrological simulation for the estimation of snowmelt contribution of Alaknanda River Basin, Uttarakhand using SWAT
Authors: Rautela Kuldeep Singh , Kumar Dilip , Gandhi Bandaru Goutham Rajeev , Kumar Ajay , Dubey Amit Kumar 
Published: January 2023

Abstract
A large population depends on water resources generated due to runoff from Himalayan River basins. They provide enough water for drinking, domestic, industrial, and irrigation. Also, these rivers have a high hydropower potential. A lack of in-depth studies has made it difficult to understand how these rivers respond hydrologically to climate change (CC) and, thus, impact the environment. In this paper, Alaknanda River Basin (ARB) has been modelled using the Soil and Water Assessment Tool (SWAT) to understand the hydrological response and assess its water balance components. The result shows that the basin's water yield and evapotranspiration (ET) vary from 58–63% and 34–39% of total precipitation. The average annual contribution of snowmelt to the total riverine flow will range from 20–24% throughout the simulation period. SFTMP, TLAPS, SMTMP, CN2, SMFMX, and GW_DELAY is found to be most sensitive at the significance level of less than 0.05, showing the contribution of the snowmelt is significant in streamflow, while delay in the groundwater will affect the contribution of surface runoff and groundwater in the streamflow. Based on the results, it is highly recommended that the spatial and temporal hydro-meteorological should be investigated in-depth to find out the actual water potential of the basin.
Tertiary treatment of coke-oven wastewater using suspended and immobilized whole live cells of constructed bacterial–microalgal consortium: modeling and optimization using ANN–GA hybrid methodology
Authors: Rai Abhilasha , Bishayee Bhaskar , Dey Annasha , Kumar Abhay , Lahiri Sandip Kumar , Chakrabarty Jitamanyu , Dutta Susmita 
Published: January 2023

Abstract
Coke-oven wastewater (CW), containing an array of toxic pollutants above permissible limits even after conventional primary and secondary treatment, needs a tertiary (polishing) step to meet the statutory limit. In the present study, a suitable bacterial–microalgal consortium (Culture C) was constructed using bacterial (Culture B: Bacillus sp. NITD 19) and microalgal (Culture A: a consortium of Chlorella sp. and Synechococcus sp.) cultures at different ratios (v/v) and the potential of these cultures for tertiary treatment of CW was assessed. Culture C4 (Culture B:Culture A = 1:4) with inoculum size: 10% (v/v) was selected for the treatment of wastewater since the maximum growth (3.08 ± 0.57 g/L) and maximum chlorophyll content (4.05 ± 0.66 mg/L) were achieved for such culture in PLE-enriched BG-11 medium. During treatment of real secondary treated coke-oven effluent using Culture C4 in a closed photobioreactor, the removal of phenol (80.32 ± 2.76%), ammonium ions (47.85 ± 1.83%), fluoride (65.0 ± 4.12%), and nitrate (39.45 ± 3.42%) was observed after 24 h. In a packed bed bioreactor containing immobilized C4 culture, the maximum removal was obtained at the lowest flow rate (20 mL/h) and highest column bed height (20 cm). Artificial intelligence-based techniques were used for modeling and optimization of the process.
Ketorolac Tromethamine Loaded Nano-Spray Dried Nanoparticles: Preparation, Characterization, Cell Viability, COL1A1 Gene Simulation and Determination of Anti-inflammatory Activity by In vivo HET-CAM Assay
Authors: Öztürk A.A. , Çevikelli T. , Tilki E.K. , Güven U.M. , Kıyan H.T. 
Published: January 2023

Background:Ketorolac tromethamine (KT) is a non-steroidal anti-inflammatory drug from the heteroaryl acetic acid derivatives family. The most widely used new nanotechnological approaches for topical drug delivery are polymeric nanoparticles (NPs).Objective:Successful results have been obtained with low doses in many treatments, such as cancer, antimicrobial, pain, made with nanoparticle formulations of drug active ingredients.Methods:NPs were prepared using Nano Spray-Dryer. The cytotoxicity of the optimum formulation in BJ (ATCC® CRL-2522™) human fibroblast cells was determined by the WST- 1 method and the gene activity was elucidated by mRNA isolation and real-time polymerase chain reaction (RT-PCR). The in vivo HET- CAM assay was performed for anti-inflammatory activity.Results:NPs presented PDI values lower than 0.5, and therefore particle size distribution was decided to be monodisperse. Positive zeta potential values of NPs highlighted the presence of the cationic ammonium group of Eudragit® RS 100. The release rates observed from KT-NP coded formulations after 24 hours were 78.4%±2.9, demonstrating extended release from all formulations, relative to pure KT. The lowest concentration of KT-NP increased fibroblast cell proliferation higher than the highest concentration of KT. The 5-fold increased effect of KT-NP formulation on collagen gene expression compared to KT is also related to the enhanced anti-inflammatory effect in line with the in vivo HET-CAM assay results.Conclusion:With the obtained cell viability, gene expression, and HET-CAM results, it has the hope of a successful nano-topical formulation, especially in both wound healing and anti-inflammatory treatment.
Cerium Oxide Nanoparticles Ameliorate Oxidative Stress, Inflammation,
and Pain Behavior in Neuropathic Rats
Authors: Forouzanfar Fatemeh , Pourbagher-Shahri Ali Mohammad , Darroudi Majid , Sadeghi Mahmood , Vafaee Farzaneh , Moghadam Omid Fakharzadeh , Mashhad Negar Moghaddas , Ghazavi Hamed , Khorrami Mohammad Bagher 
Published: January 2023

Background:
Neuropathic pain originating from a dysfunction in the nervous system is
often intractable and chronic. Recently, several studies using nanoparticles suggested a new way to
control neuropathic pain. This study intended to explore the potential neuroprotective effect of Cerium
Oxide Nanoparticles (CNPs) synthesized by pullulan in neuropathic pain in rats.

Methods:
On the right common sciatic nerve of male Wistar rats, the chronic constriction injury
(CCI) procedure was used to establish a neuropathic pain model. CNPs were injected into the caudal
vein of the rat. Behavioral methods were used to detect mechanical allodynia, cold allodynia,
and thermal hyperalgesia in rats. Besides, inflammation factors, including tumor necrosis factor
(TNF)-α, interleukin (IL)-1β, nitric oxide (NO), and markers of oxidative stress, including
Malondialdehyde (MDA) and total thiol, were measured in the spinal cord segment of rats.

Results:
In rats with CCI, mechanical allodynia, cold allodynia, and thermal hyperalgesia developed,
which improved when the rats were administered CNPs. Spinal cord specimens of CCI rats
had elevated inflammation and oxidative stress status (↑IL-1β, ↑TNF-α, ↑NO, ↑MDA) and decreased
antioxidative levels (↓total thiol). As a result of CNPs treatment, these changes were reversed
in the spinal cord specimens.

Conclusion:
CNPs alleviate neuropathic pain by exhibiting antioxidative and anti-inflammatory
activities.
Availability, Functionality, and Safety as well as Quality Control of
Hepatocytes as Seeding Cells in Liver Regenerative Medicine: State of the
Art and Challenges
Authors: Lian Run-Tong , Zhuang An-Qi , Yue Jing , Chen Yan , Ma Kui-Fen , Wu Yi-Hang 
Published: January 2023

Abstract:
Hepatic disease is one of the most common causes of death worldwide and has become a global
health problem. Liver transplantation is the only effective treatment strategy for patients with hepatic
function failure, but the insufficient number of donated healthy livers is the main obstacle limiting this
process. To alleviate the demand for donor's livers, alternative approaches are being actively explored
using liver tissue engineering principles. Liver tissue engineering consists of three elements, including
seeding cells, extracellular matrix, and bioreactors. Among them, seeding cell is the most key factor. In
this regard, hepatocyte-based tissue engineering can overcome the above shortages for tissue repair and
regeneration in hepatic disorders. Primary human hepatocytes in liver regenerative medicine are the most
preferred seeding cells, although limited access to a sufficient number of functional hepatocytes are a
major issue due to the difficulties in long-term function maintenance of hepatocyte as well as the lack of
availability of healthy donors. Hepatocyte-like cells (HLCs), derived from various stem cells, including
non-liver-derived stem cells and liver-derived stem cells, as well as trans-differentiation of other cell
types, may provide adequate cell sources and could replace primary human hepatocytes as seeding cells.
However, it is still a great difficulty that HLCs generated by stem cell differentiation meet the quality
required for clinical therapy. Furthermore, none of the standardized protocols to generate high-quality
HLCs is available. Whether primary hepatocytes or HLCs are from various sources, preventing the functional
deterioration of hepatocytes or generating fully functional hepatocytes is also a big challenge, respectively.
In addition, the adoptions of three-dimensional co-culture systems and some small-molecule
compounds contribute to maintaining the hepatic functionality of primary hepatocytes and enhancing the
liver-specific functions of HLCs. In short, hepatocyte-based liver regenerative medicine is an attractive
alternative strategy for liver diseases, notwithstanding some challenges still exist from bench to bedside.
This review summarizes the current status, issues, and challenges in availability, functionality, and safety,
as well as quality control of seeding hepatocytes with regard to liver tissue engineering in regenerative
medicine for the treatment of liver disorders.
Imidazole and Biphenyl Derivatives as Anti-cancer Agents for Glioma Therapeutics:
Computational Drug Repurposing Strategy
Authors: Murali Poornimaa , Karuppasamy Ramanathan 
Published: January 2023

Background:
Targeting mutated isocitrate dehydrogenase 1 (mIDH1) is one of the key therapeutic strategies
for the treatment of glioma. Few inhibitors, such as ivosidenib and vorasidenib, have been identified as selective inhibitors
of mIDH1. However, dose-dependent toxicity and limited brain penetration of the blood-brain barrier remain
the major limitations of the treatment procedures using these inhibitors.

Objective:
In the present study, computational drug repurposing strategies were employed to identify potent mIDH1-
specific inhibitors from the 11,808 small molecules listed in the DrugBank repository.

Methods:
Tanimoto coefficient (Tc) calculations were initially used to retrieve compounds with structurally similar
scaffolds to ivosidenib. The resultant compounds were then subjected to molecular docking to discriminate the binders
from the non-binders. The binding affinities and pharmacokinetic properties of the screened compounds were examined
using prime Molecular Mechanics-Generalized Born Surface Area (MM-GBSA) and QikProp algorithm, respectively.
The conformational stability of these molecules was validated using 100 ns molecular dynamics simulation.

Results:
Together, these processes led to the identification of three-hit molecules, namely DB12001, DB08026, and
DB03346, as potential inhibitors of the mIDH1 protein. Of note, the binding free energy calculations and MD simulation
studies emphasized the greater binding affinity and structural stability of the hit compounds towards the mIDH1
protein.

Conclusion:
The collective evidence from our study indicates the activity of DB12001 against recurrent glioblastoma,
which, in turn, highlights the accuracy of our adapted strategy. Hence, we hypothesize that the identified lead molecules
could be translated for the development of mIDH1 inhibitors in the near future.
Impact of Yoga on Immune Response with Special Reference to COVID- 19: A Review of Randomized Controlled Trials
Authors: Mishra Neha , Mishra Saurabh 
Published: January 2023

Abstract:Like other viruses, severe acute respiratory syndrome coronavirus-2 (SARS-CoV- 2) continuously evolves due to mutations during genome replication. These mutations lead to various new variants and may alter the pathogenicity, immune escape capacity, and re-infection risk. In addition, it is also evident that a compromised immune system increases the susceptibility and severity of the disease. Therefore, strengthening the immune system is considered to combat Covid. Yoga is an ancient practice used for strengthening the body and mind. There are abundant studies that focus on yoga's benefits for stress management; however, lesser studies observed the effects of yoga on the immune system. Therefore, the present study aims to compile randomized controlled studies on the role of yoga in immunity and COVID-19. Our finding reported moderate evidence for the benefits of yoga in the levels of inflammatory markers, such as C-reactive protein (CRP), and cytokines, such as interleukin (IL) and tumor necrosis factor-alpha (TNF-α). However, lesser shreds of evidence were observed on the effect of yoga on innate and adaptive immunity. Our finding reported a paucity of studies examining the impact of yoga on the immunity of Covid patients. Thus, its efficacy as a potential adjuvant to attenuate Covid-19 infection has yet to be proven. Further extensive studies are recommended to draw the inference on the impact of yoga on COVID-19.