bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three weeks of mental training changes physiological outcomes during a time trial to exhaustion
Authors: VanHaitsma Timothy A. , Gonzalez Stephen P. , Kajitani Sten , Gabriano Emma , Hoiosen Gavin E. , Oldach Michael C. , Kingsley Karly L. 
Published: May 2023

AbstractMental training (MT) can increase endurance performance. The purpose of this study was to examine the minimum dose of mental training needed to increase performance and elucidate the physiological mechanisms underlying this improvement. In a randomized between groups pre-test–post-test design, 33 participants visited the lab on 6 separate days. A VO2peak with ventilatory threshold (VT) was performed on day 1. The subsequent visits consisted of time trials to exhaustion (TTE) performed at 10% above VT. Between visit 3 and 6, the MT group (n = 16) watched a video for 10–15 min each day for 3 weeks, while the control group (CON; n = 17) did no mental training. Heart rate (HR), rate of perceived exertion (RPE), VAS scores for pain and fatigue, electromyography, and metabolic and neuromuscular data were collected and recorded during the time trials. The GRIT-S and CD-RISC 10 surveys were completed before study days 3 and 6. TTE increased significantly for MT beginning after 2 weeks (10.0 ± 13.1%) with no further change after 3 weeks (10.4 ± 13.2). TTE also significantly decreased during the last TTE for CON (−10.3 ± 12.7). VO2, ventilation, and frequency of breathing were significantly reduced in the latter stages of the TTE for MT. EMG was also significantly decreased for MT as compared for CON throughout the trial. Three weeks of mental training improves performance by reducing EMG, decreasing activation of the muscle and reducing metabolic factors during the latter stages of exercise.
Transformative adaptation through nature-based solutions: a comparative case study analysis in China, Italy, and Germany
Authors: Scolobig Anna , Linnerooth-Bayer JoAnne , Pelling Mark , Martin Juliette G. C. , Deubelli Teresa M. , Liu Wei , Oen Amy 
Published: May 2023

AbstractThis paper explores how claims for transformative adaptation toward more equitable and sustainable societies can be assessed. We build on a theoretical framework describing transformative adaptation as it manifests across four core elements of the public-sector adaptation lifecycle: vision, planning, institutional frameworks, and interventions. For each element, we identify characteristics that can help track adaptation as transformative. Our purpose is to identify how governance systems can constrain or support transformative choices and thus enable targeted interventions. We demonstrate and test the usefulness of the framework with reference to three government-led adaptation projects of nature-based solutions (NBS): river restoration (Germany), forest conservation (China), and landslide risk reduction (Italy). Building on a desktop study and open-ended interviews, our analysis adds evidence to the view that transformation is not an abrupt system change, but a dynamic complex process that evolves over time. While each of the NBS cases fails to fulfill all the transformation characteristics, there are important transformative elements in their visions, planning, and interventions. There is a deficit, however, in the transformation of institutional frameworks. The cases show institutional commonalities in multi-scale and cross-sectoral (polycentric) collaboration as well as innovative processes for inclusive stakeholder engagement; yet, these arrangements are ad hoc, short-term, dependent on local champions, and lacking the permanency needed for upscaling. For the public sector, this result highlights the potential for establishing cross-competing priorities among agencies, cross-sectoral formal mechanisms, new dedicated institutions, and programmatic and regulatory mainstreaming.
Altered Distribution of SNARE Proteins in Primary Neurons Exposed to Different Alpha-Synuclein Proteoforms
Authors: Brolin Emma , Ingelsson Martin , Bergström Joakim , Erlandsson Anna 
Published: May 2023

AbstractGrowing evidence indicates that the pathological alpha-synuclein (α-syn) aggregation in Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) starts at the synapses. Physiologic α-syn is involved in regulating neurotransmitter release by binding to the SNARE complex protein VAMP-2 on synaptic vesicles. However, in which way the SNARE complex formation is affected by α-syn pathology remains unclear. In this study, primary cortical neurons were exposed to either α-syn monomers or preformed fibrils (PFFs) for different time points and the effect on SNARE protein distribution was analyzed with a novel proximity ligation assay (PLA). Short-term exposure to monomers or PFFs for 24 h increased the co-localization of VAMP-2 and syntaxin-1, but reduced the co-localization of SNAP-25 and syntaxin-1, indicating a direct effect of the added α-syn on SNARE protein distribution. Long-term exposure to α-syn PFFs for 7 d reduced VAMP-2 and SNAP-25 co-localization, although there was only a modest induction of ser129 phosphorylated (pS129) α-syn. Similarly, exposure to extracellular vesicles collected from astrocytes treated with α-syn PFFs for 7 d influenced VAMP-2 and SNAP-25 co-localization despite only low levels of pS129 α-syn being formed. Taken together, our results demonstrate that different α-syn proteoforms have the potential to alter the distribution of SNARE proteins at the synapse.
Graphical Abstract
Linking the effects of nursery light and fertilization and post-transplant first summer irrigation on the field survival and growth of Pinus pinaster seedlings
Authors: Al Pavel Muha Abdullah , Bravo Felipe , Ordóñez Cristóbal , Rodríguez-García Encarna 
Published: May 2023

AbstractSeedling size-related functional attributes of Mediterranean forest species are critical traits to be considered in nursery practices aimed at improving the survival and performance of seedlings exposed to summer drought in dryland reforestation projects. We looked at how nursery light regimes and nitrogen fertilization affected the survival and performance of Pinus pinaster Ait. under contrasting post-planting watering regimes. Seedlings grown in the nursery for 13 months were given low or high nitrogen doses and exposed to three light regimes (full sun, medium light and low light). After outplanting, half of the seedlings were watered fortnightly during the hottest months of the first summer and the other half were left unwatered. Seedling size-related variables (shoot height, basal diameter, stem volume and sturdiness quotient) were measured at the beginning and at the end of the study, and summer drought survival was checked three times. Our results showed that fertilization and the nursery light regime influenced initial seedling quality and transplant stress. Post-planting first summer watering also increased seedling survival probability. The medium-light and especially the full-sunlight regimes in the nursery decreased the probability of seedling mortality after outplanting. However, a low-light regime in the nursery negatively affected plant traits and increased the probability of post-planting seedling mortality. In comparison with low-N seedlings, high-N fertilization increased post-planting stress (evaluated in the change of sturdiness quotient after one field growth season), but did not affect survival.
Judd–Ofelt and luminescence properties of Dysprosium and Terbium doped bismuth-borate glass system
Authors: Ratep Asmaa , Kashif Ismail 
Published: May 2023

AbstractAbsorption spectra of Tb3+ ions from the ground state 7F6 revealed three absorption bands. In addition to the six absorption bands for Dy3+ ions observed in the visible and near-infrared ranges. The optical band gape of sample free from Dy2O3 (4 mol% Tb4O7) smaller than other samples containing Dy2O3. The oscillator strengths and Judd–Ofelt parameters (Ω2, Ω4, Ω6) for reported Dy3+ and Tb3+ absorption transitions are estimated, and they do not follow a same trend depend on the substitution between Dy and Tb. The photoluminescence excitation of the Dy3+ at λem = 575 nm reveals the presence of a Tb3+ peak at 375 nm. The emission spectra of the glasses show that only the λem = 575 nm increases due to the excitation of the Tb3+ at λem = 545 nm, indicating effective energy transfer (ET) from the Tb3+ to Dy3+ in sample containing Dy2O3 up to 2 mol% and then changed from the Dy3+ to Tb3+. An efficient yellow luminescence arises from the activator's emitting centers. ET predicts the sensitizer's luminescence degradation and the activator's luminescence growth. The color coordinates and the correlated color temperature (CCT) indicate that the glass samples are suitable for white, yellow-green, and blue-light emission depend on the energy of excitation.
Mechanism of Insoluble Aggregate Formation in a Reconstituted Solution of Spray-Dried Protein Powder
Authors: Tao Yeqing , Chen Yuan , Howard Wesley , Ibrahim Mariam , Patel Sajal M. , McMahon William P. , Kim Yoen Joo , Delmar Jared A. , Davis Darryl 
Published: May 2023

Abstract
Background
Spray-drying is considered a promising alternative drying method to lyophilization (freeze-drying) for therapeutic proteins. Particle counts in reconstituted solutions of dried solid dosage forms of biologic drug products are closely monitored to ensure product quality. We found that high levels of particles formed after reconstitution of protein powders that had been spray-dried under suboptimal conditions.

Methods
Visible and subvisible particles were evaluated. Soluble proteins in solution before spray-drying and in the reconstituted solution of spray-dried powder were analyzed for their monomer content levels and melting temperatures. Insoluble particles were collected and analyzed by Fourier transform infrared microscopy (FTIR), and further analyzed with hydrogen-deuterium exchange (HDX).

Results
Particles observed after reconstitution were shown not to be undissolved excipients. FTIR confirmed their identity as proteinaceous in nature. These particles were therefore considered to be insoluble protein aggregates, and HDX was applied to investigate the mechanism underlying aggregate formation. Heavy-chain complementarity-determining region 1 (CDR-1) in the aggregates showed significant protection by HDX, suggesting CDR-1 was critical for aggregate formation. In contrast, various regions became more conformationally dynamic globally, suggesting the aggregates have lost protein structural integrity and partially unfolded after spray-drying.

Discussion
The spray-drying process could have disrupted the higher-order structure of proteins and exposed the hydrophobic residues in CDR-1 of the heavy chain, contributing to the formation of aggregate through hydrophobic interactions upon reconstitution of spray-dried powder. These results can contribute to efforts to design spray-dry resilient protein constructs and improve the robustness of the spray-drying process.
Arbuscular mycorrhiza: advances and retreats in our understanding of the ecological functioning of the mother of all root symbioses
Authors: Kuyper Thomas W. , Jansa Jan 
Published: May 2023

Abstract

Background
Arbuscular mycorrhizal (AM) symbiosis has been referred to as the mother of all plant root symbioses as it predated the evolution of plant roots. The AM research is a multidisciplinary field at the intersection of soil science, mycology, and botany. However, in recent decades the nature and properties of soils, in which the AM symbiosis develops and functions, have received less attention than desired.

Scope
In this review we discuss a number of recent developments in AM research. We particularly cover the role of AM symbiosis in acquisition of phosphorus, nitrogen, heavy metals and metalloids, as well as water by plants from soil; mycorrhizal effects on plant nutritional stoichiometry and on the carbon cycle; the hyphosphere microbiome; so-called facultative mycorrhizal plants; explanations for lack of mycorrhizal benefit; common mycorrhizal networks; and arbuscular and ectomycorrhizal ecosystems.

Conclusion
We reflect on what has previously been described as mycorrhizal ‘dogmas’. We conclude that these are in fact generalisations on the AM symbiosis that are well supported by multiple studies, while admitting that there potentially is a geographical bias in mycorrhizal research that developed in temperate and boreal regions, and that research in other ecosystems might uncover a greater diversity of viable mycorrhizal and non-mycorrhizal strategies than currently acknowledged. We also note an increasing tendency to overinterpret data, which may lead to stagnation of some research fields due to lack of experiments designed to test the mechanistic basis of processes rather than cumulating descriptive studies and correlative evidences.
Back motion in unridden horses in walk, trot and canter on a circle
Authors: Egenvall Agneta , Engström Hanna , Byström Anna 
Published: May 2023

AbstractEquine back function is of concern to riders, as well as to veterinarians and physiotherapists; these groups may benefit from knowledge about spinal motion on the circle. This descriptive and comparative study aimed to quantify equine neck, back and pelvic motion in walk, trot and canter on a 9 m circle. Sixteen healthy horses in training, of varying breed and conformation, were measured using optical motion capture (150 Hz), with optical markers on the poll, withers, T15, tubera coxae and lumbosacral joint. Cervicothoracic and thoracolumbar flexion–extension and lateral bending, and pelvic roll, pitch and yaw, were statistically evaluated using mixed models. Motion patterns showed distinct differences between gaits, but were generally similar between horses. The thoracolumbar back was bent towards the inside of the circle (stride mean 5-6º for all gaits). The cervicothoracic spine was more flexed in walk (18°), and more extended in canter (-4—-8°), compared to trot (6–7°), whereas the thoracolumbar spine was slightly less extended in canter than in walk. Thoracolumbar flexion–extension range of motion (ROM) increased from walk (4°) to canter (9°), as did pelvic pitch ROM (walk 7° and canter 15–16°), while back lateral bending ROM and pelvic yaw ROM were lowest in trot. Taken together, the study findings suggest that neck and back motion patterns on the circle reflect an interaction between the constraints of circular movement, and the mechanics and characteristics of each gait.
Damages and legal costs under the Enforcement Directive in the light of rulings of the European Court of Justice
Authors: Szczepanowska-Kozłowska Krystyna 
Published: May 2023

AbstractThe possibility of compensation for damage caused by an infringement of intellectual property rights and of claiming the recovery of legal costs and other expenses incurred in connection with enforcing such rights is crucial for the effectiveness of their protection. Uniform rules in this respect have been introduced by Enforcement Directive. (Directive 2004/48/EC of the European Parliament and of the Council of 29.4.2004 on the enforcement of intellectual property rights [2004] OJ L157/45 (Directive 2004/48).) The purpose of this article is to analyse to what extent the case-law of the European Court of Justice to date has made it possible to give an unambiguous answer regarding the model of implementation of Directive 2004/48 which meets the requirements of proportionality and, at the same time, of the effectiveness of the protective measures envisaged for intellectual property rights.
Evaluation of antioxidant and antibacterial potential of honey produced from stimulative diet fed bee colonies
Authors: Kumari Indu , Hajam Younis Ahmad , Thiyagarajan Kalaiyarasan , Giri Arup , Kumar Rajesh 
Published: May 2023

AbstractBee's honey is one of the greatest natural gifts to humankind, and Indian Ayurveda emphasises the various applications of honey, viz. obesity, burns, cuts, wounds, dermatitis etc. However, all the applications of honey are based on their chemical composition, but they vary depending upon the botanical, geographical origins, and bee feed. With this background, the current study aimed to evaluate the quality of honey produced by the stimulative diets ingested by honey bees (Apis mellifera). At the onset, stimulative diets were served to honey bees for 1 year, and an adequate volume of honey samples was collected before and after the ingestion of stimulative diets. After that, the honey samples were used to study the antioxidant activity and antibacterial potential as per standard protocol. The results revealed that the antioxidant activity of post-feeding honey samples exhibited a higher rate, i.e., 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Pre-feeding: 88.06 ± 0.214 and post-feeding: 91.79 ± 0.609 μmol/litre), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Pre-feeding: 20.55 ± 0.336 and post-feeding: 29.01 ± 1.114%), and ferric reducing ability of plasma (FRAP) (Pre-feeding: 0.296 ± 0.039 and post-feeding: 0.396 ± 0.001 mg TE/100 gm). Similarly, a high rate of antibacterial efficiency was shown in post-feeding honey samples against E. coli at a concentration of 800 µg/mL. It was shown with considerable antibacterial activity against Salmonella enterica as well. Overall, we have demonstrated honey's antioxidant and antibacterial effects and may have therapeutic potential as honey.
The response of aquatic ecosystems to the interactive effects of stratospheric ozone depletion, UV radiation, and climate change
Authors: Neale P. J. , Williamson C. E. , Banaszak A. T. , Häder D.-P. , Hylander S. , Ossola R. , Rose K. C. , Wängberg S.-Å. , Zepp R. 
Published: May 2023

AbstractVariations in stratospheric ozone and changes in the aquatic environment by climate change and human activity are modifying the exposure of aquatic ecosystems to UV radiation. These shifts in exposure have consequences for the distributions of species, biogeochemical cycles, and services provided by aquatic ecosystems. This Quadrennial Assessment presents the latest knowledge on the multi-faceted interactions between the effects of UV irradiation and climate change, and other anthropogenic activities, and how these conditions are changing aquatic ecosystems. Climate change results in variations in the depth of mixing, the thickness of ice cover, the duration of ice-free conditions and inputs of dissolved organic matter, all of which can either increase or decrease exposure to UV radiation. Anthropogenic activities release oil, UV filters in sunscreens, and microplastics into the aquatic environment that are then modified by UV radiation, frequently amplifying adverse effects on aquatic organisms and their environments. The impacts of these changes in combination with factors such as warming and ocean acidification are considered for aquatic micro-organisms, macroalgae, plants, and animals (floating, swimming, and attached). Minimising the disruptive consequences of these effects on critical services provided by the world’s rivers, lakes and oceans (freshwater supply, recreation, transport, and food security) will not only require continued adherence to the Montreal Protocol but also a wider inclusion of solar UV radiation and its effects in studies and/or models of aquatic ecosystems under conditions of the future global climate.
Graphical abstract
Vehicle detection systems for intelligent driving using deep convolutional neural networks
Authors: Abiyev Rahib , Arslan Murat 
Published: May 2023

AbstractIn the paper, a vision-based vehicle identification system is proposed for autonomous intelligent car driving. The accurate detection of obstacles (vehicles) during intelligent car driving allows avoiding crashes, preventing accidents, saving people’s lives and reducing harm. The vehicle detection system, which uses low-quality images captured by a monocular video camera mounted at the front of the car, is based on convolutional neural networks (CNN). The CNN can extract global features of the images using convolutional layers and achieves more accurate, and faithful contours of vehicles. The CNN structure proposed in the paper provides high-accuracy detection of vehicle images. The experiments that have been performed using GTI dataset demonstrate that the CNN-based vehicle detection system achieves very accurate results and is more robust to different variations of images.
The Teachings of Mistle Thrush and Kingfisher
Authors: Reason Peter , Gillespie Sarah 
Published: May 2023

AbstractWhat would it be like to learn to live in and experience a world of sentient beings rather than inert objects? How can we learn to awarely participate in a world of communication and interaction, in which trees, crows and rivers may grace us with a response to our attention and our call? How do we learn not just to know this intellectually but ‘proved upon our pulses’, as John Keats put it. As artist and writer, we reflect on the contribution our practices can have to the ecological crisis of our times, drawing on living cosmos panpsychism and examples from our practice.
Comparison of quantizations of symmetric spaces: cyclotomic Knizhnik–Zamolodchikov equations and Letzter–Kolb coideals
Authors: De Commer Kenny , Neshveyev Sergey , Tuset Lars , Yamashita Makoto 
Published: May 2023

Abstract
We establish an equivalence between two approaches to quantization of irreducible symmetric spaces of compact type within the framework of quasi-coactions, one based on the Enriquez–Etingof cyclotomic Knizhnik–Zamolodchikov (KZ) equations and the other on the Letzter–Kolb coideals. This equivalence can be upgraded to that of ribbon braided quasi-coactions, and then the associated reflection operators (K-matrices) become a tangible invariant of the quantization. As an application we obtain a Kohno–Drinfeld type theorem on type

$\mathrm {B}$

braid group representations defined by the monodromy of KZ-equations and by the Balagović–Kolb universal K-matrices. The cases of Hermitian and non-Hermitian symmetric spaces are significantly different. In particular, in the latter case a quasi-coaction is essentially unique, while in the former we show that there is a one-parameter family of mutually nonequivalent quasi-coactions.
The 1939 Option Agreement and the ‘Consistent Ambivalence’ of Fascist Policies towards Minorities in the Italian New Provinces
Authors: Dalle Mulle Emmanuel , Ambrosino Alessandro 
Published: May 2023

AbstractThe 1939 Option Agreement between Italy and Germany concerning South Tyrol was the first population transfer agreement in western European history. Its analysis offers a unique opportunity to shift the focus of the historiography on interwar minority questions from eastern to western Europe, thus challenging the lingering view of eastern Europe as a land of endemic ethnic heterogeneity and conflict. Furthermore, the 1939 Option illuminates a form of ‘consistent ambivalence’ that problematizes dominant analytical frameworks concerning the management of ethnic differences. Italian fascists consistently affirmed the inevitable assimilation, and therefore inclusion, of minorities within the Italian nation, but they also deeply distrusted them. As this ambivalent attitude reached a climax in the 1939 Option, in order to understand fascist behaviour during the implementation of the agreement we need to consider the longer history of fascist attempts to homogenize the new provinces. Three features structured these attempts: a belief that the assimilation of these minorities would be inevitable; the absence of means to carry out radical solutions; and a deep-seated distrust of the minorities. Fascist policy during the Option was simultaneously more ambivalent than the current historiography suggests and more consistent with the regime's interwar homogenization policies.