bluuebunny/crossref_metadata_2025_split huggingface dataset:
Second haploidentical stem cell transplantation (HAPLO-SCT2) after relapse from a first HAPLO-SCT in acute leukaemia—a study on behalf of the Acute Leukaemia Working Party (ALWP) of the European Society for Blood and Marrow Transplantation (EBMT)
Authors: Filippini Velázquez Giuliano , Labopin Myriam , Tischer Johanna , Raiola Anna Maria , Angelucci Emanuele , Kulagin Alexander D. , Galieni Piero , Bermúdez Arancha , Bulabois Claude-Eric , Kröger Nicolaus , Díez-Martín José Luis , Kwon Mi , Nagler Arnon , Schmid Christoph , Ciceri Fabio , Mohty Mohamad 
Published: May 2023

AbstractFor patients with acute myeloid and lymphoblastic leukaemia (AML/ALL) lacking a matched sibling or unrelated donor, haploidentical stem cell transplantation (HAPLO-SCT) is increasingly used. However, available data on the treatment of relapse after HAPLO-SCT, including feasibility and efficacy of a second HAPLO-SCT (HAPLO-SCT2), is scarce. Hence, adults with AML/ALL, that had undergone HAPLO-SCT2 without ex-vivo manipulation after haematologic relapse from HAPLO-SCT1 were selected for a retrospective registry analysis. Eighty-two patients (AML, n = 63, ALL, n = 19, median follow-up: 33 months) were identified. Engraftment rate was 87%. At day +180, cumulative incidences of acute GvHD II-IV°/chronic GvHD were 23.9%/22.6%, respectively. Two-year overall survival/leukaemia-free survival (OS/LFS) were 34.3%/25.4%; 2-year non-relapse mortality (NRM) and relapse incidence (RI) were 17.6% and 57%. Leukaemia was the most frequent cause of death. Separated by disease, 2-year OS/LFS/NRM/RI were 28.7%/22.3%/16.2%/61.6% in AML, and 55.3%/38.4%/23.5%/38.2% in ALL patients. In a risk-factor analysis among patients with AML, stage at HAPLO-SCT1 and HAPLO-SCT2, and interval from HAPLO-SCT1 to relapse significantly influenced outcome. Our data demonstrate that HAPLO-SCT2 is a viable option in acute leukaemia relapse after HAPLO-SCT1. Engraftment, toxicity, risk factors and long-term outcome are comparable to data reported after allo-SCT2 in a matched donor setting.
Ti3C2Tx MXene as a growth template for amorphous RuOx in carbon nanofiber-based flexible electrodes for enhanced pseudocapacitive energy storage
Authors: Hwang Hyewon , Yang Sungeun , Yuk Seoyeon , Lee Kug-Seung , Byun Segi , Lee Dongju 
Published: May 2023

AbstractA noble surface engineering method was developed to create a binder-free flexible electrode comprising Ti3C2Tx MXene/carbon nanofibers (MCNFs) covered by amorphous RuOx with a combined electrospinning and hydrothermal process. Utilizing the hydrophilicity of the MXene on/in the MCNFs, RuOx was easily coated on the surfaces of the MCNFs through oxygen-mediated chemical bonding between the functional groups of the MXene and Ru ions. A structural analysis revealed that the MXene acted as a growth template for RuOx and that the formed RuOx had an amorphous and disordered state in the composite electrode, which impacted the electrochemical performance. The electrochemical tests showed that these composite electrodes improved the electrochemical performance, with a two-fold increase in the gravimetric capacitance (279.4 F/g at 2 mV/s) relative to that of pristine MCNFs, a wide potential window (from 0.7 to 1 V) providing a superior energy density of 8.5 Wh/kg at a power density of 85.8 W/kg, as well as long-term cycling stability (99% after 10,000 cycles). The synergetic effect of the RuOx and MXene in the composite electrodes was attributed to an enhanced pseudocapacitive reaction. Our novel electrodes and fabrication method confirm the great potential of CNF-based composites for the development of high-performance binder-free electrodes for supercapacitors.
Negative interfacial energies of dynamic polymer brush interfaces: a discussion of the free energy balance
Authors: Saito Masayuki , Ito Kohzo , Yokoyama Hideaki 
Published: May 2023

AbstractInterfacial energy is an important physical parameter for describing interfacial properties. However, quantifying the interfacial energy of a polymer–liquid interface is extremely difficult because numerous polymers reconstruct when exposed to liquids. A dynamic polymer brush is a reconstructed surface. When a hydrophobic elastomer containing amphiphilic block copolymers comes into contact with water, the copolymers spontaneously segregate at the elastomer–water interface to form a hydrophilic brush known as a dynamic polymer brush. We previously succeeded in analyzing the interfacial energy of a dynamic polymer brush interface by measuring the deformation of an ultrathin square elastomer film floating on water. However, the quantitativity remains debatable because the elastic modulus of a bulk elastomer sheet, rather than an ultrathin elastomer film, was used. In this study, we reanalyze the interfacial energy of a dynamic polymer brush system using the ultrathin-film modulus, which we recently measured via a film-on-water tensile test. Large negative interfacial energy was observed for the system of high-density stretched brushes. The free energy balance for the dynamic polymer brush system floating on water was calculated, and results validated the negative interfacial energy, which is a distinctive feature of dynamic polymer brushes.
Comparison of diabetic retinopathy severity grading on ETDRS 7-field versus ultrawide-field assessment
Authors: Attiku Yamini , Nittala Muneeswar Gupta , Velaga Swetha B. , Ramachandra Chaithanya , Bhat Sandeep , Solanki Kaushal , Jayadev Chaitra , Choudhry Netan , Orr Samantha Miyoko Ashlyn , Jiang Shangjun , He Ye , Sadda SriniVas R. 
Published: May 2023

Abstract
Objectives
To compare the diabetic retinopathy (DR) severity level determined when considering only the ETDRS 7-field region versus the entire ultrawidefield (UWF) image.

Methods
In this retrospective, cross-sectional study, UWF pseudocolor images were graded on the Eyenuk image viewing, grading, and annotation platform for the severity of DR considering only the regions within the ETDRS 7-fields as well as the entire UWF image using two different protocols: 1) the simple International Classification of Diabetic Retinopathy (ICDR) scale and 2) the more complex DRCR.net Protocol AA grading scale.

Results
A total of 250 eyes from 157 patients were included in this analysis. Six eyes (2.4%) demonstrated a discrepancy in severity level between the ETDRS 7-field region and the entire UWF image when using the ICDR classification system. The discrepancies were due to the presence of lesions [intraretinal haemorrhage (n = 2), neovascular disease (n = 4)] in the peripheral fields which were not identified in the ETDRS 7-fields. Fourteen eyes (5.6%) had a discrepancy in severity level between the ETDRS 7-field region and the entire UWF image when using the ETDRS DRSS Protocol AA grading scale. The discrepancies were due to the presence of a higher level of disease [intraretinal haemorrhage (n = 4), neovascularization (n = 4), preretinal haemorrhage (n = 2), scatter laser scars (n = 4)] in the peripheral fields.

Conclusion
Although considering regions outside of the ETDRS 7-fields altered the DR severity level assessment in <5% of cases in this cohort, significant and potentially vision-threatening lesions including neovascularization and preretinal haemorrhage were identified in these peripheral regions. This highlights the importance of evaluating the entire UWF region when assessing patients with diabetic retinopathy.
Loophole-free Bell inequality violation with superconducting circuits
Authors: Storz Simon , Schär Josua , Kulikov Anatoly , Magnard Paul , Kurpiers Philipp , Lütolf Janis , Walter Theo , Copetudo Adrian , Reuer Kevin , Akin Abdulkadir , Besse Jean-Claude , Gabureac Mihai , Norris Graham J. , Rosario Andrés , Martin Ferran , Martinez José , Amaya Waldimar , Mitchell Morgan W. , Abellan Carlos , Bancal Jean-Daniel , Sangouard Nicolas , Royer Baptiste , Blais Alexandre , Wallraff Andreas 
Published: May 2023

AbstractSuperposition, entanglement and non-locality constitute fundamental features of quantum physics. The fact that quantum physics does not follow the principle of local causality1–3 can be experimentally demonstrated in Bell tests4 performed on pairs of spatially separated, entangled quantum systems. Although Bell tests, which are widely regarded as a litmus test of quantum physics, have been explored using a broad range of quantum systems over the past 50 years, only relatively recently have experiments free of so-called loopholes5 succeeded. Such experiments have been performed with spins in nitrogen–vacancy centres6, optical photons7–9 and neutral atoms10. Here we demonstrate a loophole-free violation of Bell’s inequality with superconducting circuits, which are a prime contender for realizing quantum computing technology11. To evaluate a Clauser–Horne–Shimony–Holt-type Bell inequality4, we deterministically entangle a pair of qubits12 and perform fast and high-fidelity measurements13 along randomly chosen bases on the qubits connected through a cryogenic link14 spanning a distance of 30 metres. Evaluating more than 1 million experimental trials, we find an average S value of 2.0747 ± 0.0033, violating Bell’s inequality with a P value smaller than 10−108. Our work demonstrates that non-locality is a viable new resource in quantum information technology realized with superconducting circuits with potential applications in quantum communication, quantum computing and fundamental physics15.
High-throughput printing of combinatorial materials from aerosols
Authors: Zeng Minxiang , Du Yipu , Jiang Qiang , Kempf Nicholas , Wei Chen , Bimrose Miles V. , Tanvir A. N. M. , Xu Hengrui , Chen Jiahao , Kirsch Dylan J. , Martin Joshua , Wyatt Brian C. , Hayashi Tatsunori , Saeidi-Javash Mortaza , Sakaue Hirotaka , Anasori Babak , Jin Lihua , McMurtrey Michael D. , Zhang Yanliang 
Published: May 2023

AbstractThe development of new materials and their compositional and microstructural optimization are essential in regard to next-generation technologies such as clean energy and environmental sustainability. However, materials discovery and optimization have been a frustratingly slow process. The Edisonian trial-and-error process is time consuming and resource inefficient, particularly when contrasted with vast materials design spaces1. Whereas traditional combinatorial deposition methods can generate material libraries2,3, these suffer from limited material options and inability to leverage major breakthroughs in nanomaterial synthesis. Here we report a high-throughput combinatorial printing method capable of fabricating materials with compositional gradients at microscale spatial resolution. In situ mixing and printing in the aerosol phase allows instantaneous tuning of the mixing ratio of a broad range of materials on the fly, which is an important feature unobtainable in conventional multimaterials printing using feedstocks in liquid–liquid or solid–solid phases4–6. We demonstrate a variety of high-throughput printing strategies and applications in combinatorial doping, functional grading and chemical reaction, enabling materials exploration of doped chalcogenides and compositionally graded materials with gradient properties. The ability to combine the top-down design freedom of additive manufacturing with bottom-up control over local material compositions promises the development of compositionally complex materials inaccessible via conventional manufacturing approaches.
X-ray-to-visible light-field detection through pixelated colour conversion
Authors: Yi Luying , Hou Bo , Zhao He , Liu Xiaogang 
Published: May 2023

AbstractLight-field detection measures both the intensity of light rays and their precise direction in free space. However, current light-field detection techniques either require complex microlens arrays or are limited to the ultraviolet–visible light wavelength ranges1–4. Here we present a robust, scalable method based on lithographically patterned perovskite nanocrystal arrays that can be used to determine radiation vectors from X-rays to visible light (0.002–550 nm). With these multicolour nanocrystal arrays, light rays from specific directions can be converted into pixelated colour outputs with an angular resolution of 0.0018°. We find that three-dimensional light-field detection and spatial positioning of light sources are possible by modifying nanocrystal arrays with specific orientations. We also demonstrate three-dimensional object imaging and visible light and X-ray phase-contrast imaging by combining pixelated nanocrystal arrays with a colour charge-coupled device. The ability to detect light direction beyond optical wavelengths through colour-contrast encoding could enable new applications, for example, in three-dimensional phase-contrast imaging, robotics, virtual reality, tomographic biological imaging and satellite autonomous navigation.
Profiling the human intestinal environment under physiological conditions
Authors: Shalon Dari , Culver Rebecca Neal , Grembi Jessica A. , Folz Jacob , Treit Peter V. , Shi Handuo , Rosenberger Florian A. , Dethlefsen Les , Meng Xiandong , Yaffe Eitan , Aranda-Díaz Andrés , Geyer Philipp E. , Mueller-Reif Johannes B. , Spencer Sean , Patterson Andrew D. , Triadafilopoulos George , Holmes Susan P. , Mann Matthias , Fiehn Oliver , Relman David A. , Huang Kerwyn Casey 
Published: May 2023

AbstractThe spatiotemporal structure of the human microbiome1,2, proteome3 and metabolome4,5 reflects and determines regional intestinal physiology and may have implications for disease6. Yet, little is known about the distribution of microorganisms, their environment and their biochemical activity in the gut because of reliance on stool samples and limited access to only some regions of the gut using endoscopy in fasting or sedated individuals7. To address these deficiencies, we developed an ingestible device that collects samples from multiple regions of the human intestinal tract during normal digestion. Collection of 240 intestinal samples from 15 healthy individuals using the device and subsequent multi-omics analyses identified significant differences between bacteria, phages, host proteins and metabolites in the intestines versus stool. Certain microbial taxa were differentially enriched and prophage induction was more prevalent in the intestines than in stool. The host proteome and bile acid profiles varied along the intestines and were highly distinct from those of stool. Correlations between gradients in bile acid concentrations and microbial abundance predicted species that altered the bile acid pool through deconjugation. Furthermore, microbially conjugated bile acid concentrations exhibited amino acid-dependent trends that were not apparent in stool. Overall, non-invasive, longitudinal profiling of microorganisms, proteins and bile acids along the intestinal tract under physiological conditions can help elucidate the roles of the gut microbiome and metabolome in human physiology and disease.
Pan-cancer whole-genome comparison of primary and metastatic solid tumours
Authors: Martínez-Jiménez Francisco , Movasati Ali , Brunner Sascha Remy , Nguyen Luan , Priestley Peter , Cuppen Edwin , Van Hoeck Arne 
Published: May 2023

AbstractMetastatic cancer remains an almost inevitably lethal disease1–3. A better understanding of disease progression and response to therapies therefore remains of utmost importance. Here we characterize the genomic differences between early-stage untreated primary tumours and late-stage treated metastatic tumours using a harmonized pan-cancer analysis (or reanalysis) of two unpaired primary4 and metastatic5 cohorts of 7,108 whole-genome-sequenced tumours. Metastatic tumours in general have a lower intratumour heterogeneity and a conserved karyotype, displaying only a modest increase in mutations, although frequencies of structural variants are elevated overall. Furthermore, highly variable tumour-specific contributions of mutational footprints of endogenous (for example, SBS1 and APOBEC) and exogenous mutational processes (for example, platinum treatment) are present. The majority of cancer types had either moderate genomic differences (for example, lung adenocarcinoma) or highly consistent genomic portraits (for example, ovarian serous carcinoma) when comparing early-stage and late-stage disease. Breast, prostate, thyroid and kidney renal clear cell carcinomas and pancreatic neuroendocrine tumours are clear exceptions to the rule, displaying an extensive transformation of their genomic landscape in advanced stages. Exposure to treatment further scars the tumour genome and introduces an evolutionary bottleneck that selects for known therapy-resistant drivers in approximately half of treated patients. Our data showcase the potential of pan-cancer whole-genome analysis to identify distinctive features of late-stage tumours and provide a valuable resource to further investigate the biological basis of cancer and resistance to therapies.
Personalized RNA neoantigen vaccines stimulate T cells in pancreatic cancer
Authors: Rojas Luis A. , Sethna Zachary , Soares Kevin C. , Olcese Cristina , Pang Nan , Patterson Erin , Lihm Jayon , Ceglia Nicholas , Guasp Pablo , Chu Alexander , Yu Rebecca , Chandra Adrienne Kaya , Waters Theresa , Ruan Jennifer , Amisaki Masataka , Zebboudj Abderezak , Odgerel Zagaa , Payne George , Derhovanessian Evelyna , Müller Felicitas , Rhee Ina , Yadav Mahesh , Dobrin Anton , Sadelain Michel , Łuksza Marta , Cohen Noah , Tang Laura , Basturk Olca , Gönen Mithat , Katz Seth , Do Richard Kinh , Epstein Andrew S. , Momtaz Parisa , Park Wungki , Sugarman Ryan , Varghese Anna M. , Won Elizabeth , Desai Avni , Wei Alice C. , D’Angelica Michael I. , Kingham T. Peter , Mellman Ira , Merghoub Taha , Wolchok Jedd D. , Sahin Ugur , Türeci Özlem , Greenbaum Benjamin D. , Jarnagin William R. , Drebin Jeffrey , O’Reilly Eileen M. , Balachandran Vinod P. 
Published: May 2023

AbstractPancreatic ductal adenocarcinoma (PDAC) is lethal in 88% of patients1, yet harbours mutation-derived T cell neoantigens that are suitable for vaccines 2,3. Here in a phase I trial of adjuvant autogene cevumeran, an individualized neoantigen vaccine based on uridine mRNA–lipoplex nanoparticles, we synthesized mRNA neoantigen vaccines in real time from surgically resected PDAC tumours. After surgery, we sequentially administered atezolizumab (an anti-PD-L1 immunotherapy), autogene cevumeran (a maximum of 20 neoantigens per patient) and a modified version of a four-drug chemotherapy regimen (mFOLFIRINOX, comprising folinic acid, fluorouracil, irinotecan and oxaliplatin). The end points included vaccine-induced neoantigen-specific T cells by high-threshold assays, 18-month recurrence-free survival and oncologic feasibility. We treated 16 patients with atezolizumab and autogene cevumeran, then 15 patients with mFOLFIRINOX. Autogene cevumeran was administered within 3 days of benchmarked times, was tolerable and induced de novo high-magnitude neoantigen-specific T cells in 8 out of 16 patients, with half targeting more than one vaccine neoantigen. Using a new mathematical strategy to track T cell clones (CloneTrack) and functional assays, we found that vaccine-expanded T cells comprised up to 10% of all blood T cells, re-expanded with a vaccine booster and included long-lived polyfunctional neoantigen-specific effector CD8+ T cells. At 18-month median follow-up, patients with vaccine-expanded T cells (responders) had a longer median recurrence-free survival (not reached) compared with patients without vaccine-expanded T cells (non-responders; 13.4 months, P = 0.003). Differences in the immune fitness of the patients did not confound this correlation, as responders and non-responders mounted equivalent immunity to a concurrent unrelated mRNA vaccine against SARS-CoV-2. Thus, adjuvant atezolizumab, autogene cevumeran and mFOLFIRINOX induces substantial T cell activity that may correlate with delayed PDAC recurrence.
Genetic immune escape landscape in primary and metastatic cancer
Authors: Martínez-Jiménez Francisco , Priestley Peter , Shale Charles , Baber Jonathan , Rozemuller Erik , Cuppen Edwin 
Published: May 2023

AbstractStudies have characterized the immune escape landscape across primary tumors. However, whether late-stage metastatic tumors present differences in genetic immune escape (GIE) prevalence and dynamics remains unclear. We performed a pan-cancer characterization of GIE prevalence across six immune escape pathways in 6,319 uniformly processed tumor samples. To address the complexity of the HLA-I locus in the germline and in tumors, we developed LILAC, an open-source integrative framework. One in four tumors harbors GIE alterations, with high mechanistic and frequency variability across cancer types. GIE prevalence is generally consistent between primary and metastatic tumors. We reveal that GIE alterations are selected for in tumor evolution and focal loss of heterozygosity of HLA-I tends to eliminate the HLA allele, presenting the largest neoepitope repertoire. Finally, high mutational burden tumors showed a tendency toward focal loss of heterozygosity of HLA-I as the immune evasion mechanism, whereas, in hypermutated tumors, other immune evasion strategies prevail.
Database covering the prayer movements which were not available previously
Authors: Mihcin Senay , Sahin Ahmet Mert , Yilmaz Mehmet , Alpkaya Alican Tuncay , Tuna Merve , Akdeniz Sevinc , Korkmaz Nuray Can , Tosun Aliye , Sahin Serap 
Published: May 2023

AbstractLower body implants are designed according to the boundary conditions of gait data and tested against. However, due to diversity in cultural backgrounds, religious rituals might cause different ranges of motion and different loading patterns. Especially in the Eastern part of the world, diverse Activities of Daily Living (ADL) consist of salat, yoga rituals, and different style sitting postures. A database covering these diverse activities of the Eastern world is non-existent. This study focuses on data collection protocol and the creation of an online database of previously excluded ADL activities, targeting 200 healthy subjects via Qualisys and IMU motion capture systems, and force plates, from West and Middle East Asian populations with a special focus on the lower body joints. The current version of the database covers 50 volunteers for 13 different activities. The tasks are defined and listed in a table to create a database to search based on age, gender, BMI, type of activity, and motion capture system. The collected data is to be used for designing implants to allow these sorts of activities to be performed.