bluuebunny/crossref_metadata_2025_split huggingface dataset:
EGFR, and VEGFR inhibitory activities of the crude extract from marine algae Dictyopteris acrostichoides supported by in silico analysis and metabolic profiling
Authors: Attia Eman Zekry , Abdel-Rahman Iman A. M. , Aly Omar M. , Saber Hani , Rushdi Mohammed Ismael , Abdelmohsen Usama Ramadan 
Published: May 2023

Abstract
Ethanol extracts of Caulerpa racemosa, Dictyopteris acrostichoides, Halimeda opuntia and Polycladia myrica, were tested for their cytotoxicity against HepG2 (human hepatoma), MCF-7 (human breast adenocarcinoma), and Caco-2 (human colon adenocarcinoma) cells. Dictyopteris acrostichoides displayed cytotoxicity against HepG2, MCF-7 and Caco-2 with IC50 values of 11.65, 9.28 and 16.86 µg/mL, respectively in comparison to doxorubicin as a positive control, (IC50 = 5.72, 5.17 and 5.81 µg/mL, respectively). LC-HR-ESI-MS metabolic profiling of the D. acrostichoides extract characterized seventeen metabolites (1–17). In silico analysis indicated 1-(3-oxo-undecyldisulfanyl)-undecan-3-one (16) was the most active EGFR inhibitor, while 1-(3-Oxo-undecyldisulfanyl)-undecan-3-one (16) and di(3-acetoxy-5-undecenyl) disulfide (17) were the most active VEGFR inhibitors. Furthermore, the ethanol extract of D. acrostichoides was tested against epidermal growth factor receptor (EGFR) kinase (IC50 = 0.11 µg/mL) compared to lapatinib as a positive control, (IC50 = 0.03µg/mL) and against vascular endothelial growth factor (VEGF) (IC50 = 0.276 µg/mL) compared to sorafenib as a positive control, (IC50 = 0.049 µg/mL).
Robinin inhibits pancreatic cancer cell proliferation, EMT and inflammation via regulating TLR2-PI3k-AKT signaling pathway
Authors: Zhang wenwen , Liu Wenting , Hu Xingchen 
Published: May 2023

Abstract
Purpose To investigate the anti-tumor effect of Robinin (Toll-like receptor 2 inhibitor) in pancreatic cancer cells via regulating tumor microenvironment.Methods The effects of Robinin on cell proliferation or migration in Mia-PACA2 and PANC-1 were determined, using CCK8 or wound healing assay, respectively. The typical markers of EMT (αSMA and snail) and the inflammation markers (IL-6 and TNF-α) were all detected by western blot. CU-T12-9 (TLR2 agonist) was used to rescue Robinin’s effect. PI3k-p85α and Phosphorylated-AKT (p-AKT) were evaluated, compared to the β-actin and AKT, using western blot.Results Robinin significantly inhibited cell proliferation and migration in Mia-PACA2 and PANC-1, compared to HPNE (**P < 0.01). Robinin also attenuated the expression of α-SMA and snail in Mia-PACA2, and PANC-1 (**P < 0.01). Besides, it was found that expression of IL-6 and TNF-α were diminished in presence of Robinin in Mia-PACA2, and PANC-1 (**P < 0.01). Western blot confirmed that Robinin could target on TLR2, and further downregulated PI3k-AKT signaling pathway to exert biological function.Conclusions Robinin exerts anti-tumor effect perhaps via downregulating inflammation and EMT in pancreatic cancer cell through inhibiting TLR2-PI3k-AKT signaling pathway. Robinin may be a novel agent in adjuvant therapy of pancreatic cancer.
Synthesis, Characterization, and In Vitro Evaluation of Alpha-amylase and Alpha- glucosidase Studies of 2,3,4-triimidazole Derivatives
Authors: Abbas Sawsan K , Al-Saadi Narjis Hadi , Hanoon H. D. 
Published: May 2023

Abstract
In medicinal chemistry, employing privileged scaffolds provides researchers with a jump start in their search for novel and improved therapeutic agents. The imidazole ring, which has a variety of derivatives that have shown a broad range of biological functions, is one of these scaffolds. A new imidazole(4a-4e) derivatives were synthesized under reflex conditions or ultrasonic irradiation, and identified, by using melting point, FTIR, 1HNMR, 13CMR and mass spectra. The ultrasonic-assisted reactions were compared with the reactions under traditional heating conditions. Then the prepared compounds were investigated in vitro as antidiabetic agents using α-amylase and α- glucosidase assays with the reference drug acarbose at different concentrations (50,100,150,200 and 250µg/mL). All evaluated compounds were found to be more active than acarbose (32% − 63%) against α-amylase with percentage inhibition in the range of (49% -82%).In vitro assays of α-glucosidase activity showed a percentage inhibition ranging from ( 26%-47% ) for 4a ( 21%- 34%) for 4b (24%-44%) for 4c ( 27%-52%) for 4d and ( 29%-48%) for 4e which were lower than that of acarbose (43%-61%) at the concentration (50–200µg/mL), whereas at concentration 250µg/mL the percentage inhibition of 4a,4c and 4d with 56%,58 and 61% respectively, was closer to acarbose 66%. As a result, the investigated compounds may have α-amylase and α-glucosidase inhibitory activity and may be utilized as anti-diabetes agents.
FakeNewsDetection using Soft Computing
Authors: S AKSHAYAMATHI , B PREETHI , M KEERTHANA , S AYYASAMY 
Published: May 2023

Abstract
Reports have been around for a while and were accepted as having real results. The negative impact of rumors has increased due to the quick development of online media, so it is critical to spot them as soon as possible. Several methods have been used before offers distinct reviews based on the information's content or social context. In any event, the majority of current approaches ignore or fail to explore effectively the instance of information generating in online media, including the grouping of collaborations of internetbased media users with information through time. In this paper, we provide a novel strategy. we provide a cutting-edge method for communicating identification that is based on in-depth learning. Our approach makes use of the dynamics of how news spreads by studying how customers are portrayed and how their responses vary around the world. We want to give our customers the ability to verify the rumors and fake news they come across on social media platforms like Twitter and Facebook. The clients can get verifiable facts and continually search for the sources of information occurrences. Official organizations can conform to a situation and participants in the flow by using the constant movement variables. Experiments conducted on the Twitter and Weibo datasets demonstrate the suggested technique's execution at the decreasing edge.
The Usefulness of Mannan Antigen and Anti-mannan Anti-body in the Diagnosis of Candidemia
Authors: Akın Hicran , Cilo Burcu Dalyan , Ener Beyza , Kazak Esra , Akalın Halis 
Published: May 2023

Abstract
Background
Candida species are among the important nosocomial infection agents. Since morbidity and mortality are high in invasive candidiasis, early diagnosis is crucial. Hence, rapid serologic tests are needed for diagnosis. One of these methods is the detection of mannan antigen (Mn) and anti-mannan antibody (A-Mn) in the blood.
Objectives
In this study, we aimed to evaluate the performance of Mn and A-Mn testing in the diagnosis of candidemia.
Methods
Seventy-two patients with candidemia, 30 patients with bacteremia, 26 individuals without infection as the control group were included in the study. Candida Mn and A-Mn levels were measured by the ELISA method. Sensitivity, specificity, and negative and positive predictive values were calculated to evaluate the performance of the tests.
Results
The sensitivity and specificity of the Mn in patients with candidemia were 31.9% and 78.7%, respectively. Mn positivity was significantly higher in neutropenic candidemia patients compared with non-neutropenic candidemia patients (p = 0.026). In the A-Mn test, sensitivity and specificity were 81.9% and 42.6%, respectively, and there was no significant difference between the candidemia and bacteremia groups. It was found that the specificity increased (90.2%) when both tests were used together.
Conclusions
It was determined that the use of Mn and A-Mn tests alone did not contribute significantly to the diagnosis of candidemia, and the combined use of Mn/ A-Mn tests may be useful. It was concluded that the Mn is a more valuable diagnostic tool in the neutropenic patient group, and regular weekly Mn screening can be recommended in these patients.
Sexual Behavior, Self-declaration of Sexual Orientation, and Violence Experience: National Health Survey, Brazil, 2019
Authors: Szwarcwald Celia Landmann , Pascom Ana Roberta Pati , Júnior Paulo Roberto Borges Souza , Damacena Giseli Nogueira , Castilho Euclides Ayres 
Published: May 2023

Abstract
Background Population surveys involving the monitoring of high-risk sexual behavior have been recognized as important public health tools to control the HIV epidemic and other sexually transmitted infections (STIs).Methods Using data from the Knowledge, Attitudes, and Practices survey (PCAP-2013) and from the National Health Survey (PNS), indicators of sexual behavior were compared according to sociodemographic characteristics, including size (%) estimates of men who have sex with men (MSM) and women who have sex with women (WSW). To compare PCAP and PNS proportional distributions, the Pearson's chi-square test, adjusted by the Rao-Scott’s correction, was applied. Using data from the PNS-2019, experience of violence (verbal, physical, and sexual) was analyzed according to sexual orientation. Odds ratios (OR) were estimated by logistic regression models using each type of violence as the response variable and sexual orientation (heterosexual, homosexual/bisexual) as the independent variable.Results Size (%) estimates of MSM and WSW, obtained by direct questions from the PCAP-2013, showed lower homosexuality prevalence estimates than those resulting from the PNS-2019 self-declared sexual orientation. Significant differences were found between the MSM proportions according to the PCAP-2013 (3.7%; 95% CI 3.1–4.4%) and to the PNS-2019 (2.2%; 95% CI 1.9–2.5), and between the WSW proportions (4.6%; 95% CI 4.0-5.4%) and (2.1%; 95% CI 1.8–2.4), respectively. Regarding condom use at last sexual intercourse, no differences between the PCAP-2013 and the PNS-2019 estimates were found at the national level, but significant improvements were found for MSM, people aged 18–24 and 25–34 years, and individuals not living with a partner. Violence-related indicators showed that over 40% of homosexual/bisexual men and women have suffered some type of violence in the past 12 months. Regarding sexual violence, at least once in a lifetime, prevalence was 18.1% among MSM, with 10-fold greater odds (9.9; 95% CI 6.7–14.8) than that of heterosexual men. Among WSW, prevalence was 25.6%, with an estimated OR of 3.3 (95% CI 2.4–4.3).Conclusions The findings emphasize the importance of monitoring HIV/STI risk-related practices and violence experienced by sexual minorities at subnational levels in order to strengthen public policies focused on overcoming the persistent homophobic prejudice in the Brazilian society.
ImageBox3: No-Server Tile Serving to Traverse Whole Slide Images on the Web
Authors: Bhawsar Praphulla MS , Bremer Erich , Duggan Máire A , Chanock Stephen , Garcia-Closas Montserrat , Saltz Joel , Almeida Jonas S 
Published: May 2023

Abstract
Background
Whole slide imaging (WSI) has become the primary modality for digital pathology data. However, due to the size and high-resolution nature of these images, they are generally only accessed in smaller sections or “tiles” via specialized platforms, most of which require extensive setup and/or costly infrastructure. These platforms typically also need a copy of the images to be locally available to them, potentially causing issues with data governance and provenance.
Results
To address these concerns, we developed ImageBox3, an in-browser tiling mechanism to enable zero-footprint traversal of remote WSI data. All computation is performed client-side without compromising user governance, operating public and private images alike as long as the storage service supports HTTP range requests (standard in Cloud storage and most web servers).
Conclusion
ImageBox3 thus removes significant hurdles to WSI operation and effective collaboration, allowing for the sort of democratized analytical tools needed to establish participative, FAIR digital pathology data commons.
Availability:
code - https://github.com/episphere/imagebox3 ;
fig1 (live) - https://episphere.github.io/imagebox3/demo/scriptTag ;
fig2 (live) - https://episphere.github.io/imagebox3/demo/serviceWorker ;
fig 3 (live) - https://observablehq.com/@prafulb/imagebox3-in-observable .
Lyso-phosphatidylethanolamine triggers immunity against necrotrophs by promoting JA-signaling and ROS-homeostasis
Authors: Voelz Ronny , Kim Ki-Tae , Alazem Mazen , Harries William , Hwang Sungkee , Lee Yong Hwan 
Published: May 2023

Abstract
Modulation of the plant defense response by bioactive molecules is of increasing interest. However, despite plant cell lipids being one of the major cellular components, their role in plant immunity remains elusive. We found that the exogenous application of the cell-membrane localized phospholipid lyso-phosphatidylethanolamine (LPE) reprograms the plant transcript profile in favor of defense-associated genes thereby priming the plant immune system. Exogenous LPE application at different Arabidopsis accessions increases resistance against the necrotrophic pathogens, Botrytis cinerea and Cochliobolus heterostrophus. We found that the immunity-promoting effect (IPE) of LPE is repealed in the jasmonic acid (JA) receptor mutant coi1, but multiplied in the JA-hypersensitive mutant feronia (fer-4). The JA-signaling repressor JAZ1 is degraded following LPE administration, suggesting that JA-signaling is promoted by LPE. Following LPE-treatment, reactive oxygen species (ROS) accumulation is affected in coi1 and fer-4. The application of ROS-scavengers compromises the LPE-mediated immune response suggesting that ROS are crucial for the LPE-mediated IPE. Moreover, FER signaling inhibitors of the RALF family are strongly expressed after LPE application, and RALF23 is internalized in stress granules, suggesting the LPE-mediated repression of FER-signaling by promoting RALF function. The in-situ increase of LPE-abundance in the LPE-biosynthesis mutant lpeat1 and lpeat2 elevates plant resistance to B. cinerea, in contrast to the endogenous LPE-deficient mutant pla2-alpha. We show that LPE increases plant resistance against necrotrophs by promoting JA-signaling and ROS-homeostasis, thereby paving the way for the LPE-targeted genomic engineering of crops to raise their ability to resist biotic threats.
Moe-Phobia and Conservative Mentality
Authors: Matsui Tetsuya 
Published: May 2023

Abstract
This paper focuses on moe-phobia, sexuality, and psychological resistance toward manga-like virtual agents. Moe-phobia is psychological disgust to sexual manga-like virtual agents. In a prior study, it was reported that the agents’ appearance and context had an interaction effect on moe-phobia in movies. This paper has two aims: to verify whether the interaction between appearance and context shown in previous studies is also observed in an experiment using still pictures, and to investigate the correlation between the degree to which participants exhibit moe-phobia and their political leanings. I conducted an experiment with three factors and two conditions. The factors were the agents’ appearance (swimsuit, white coat), context (pool, science museum), and the participants’ gender. The participants answered questions related to the agents’ sexuality and appropriateness perceived as well as a scale of conservative tendency. As a result, an interaction between appearance and context was observed. Also, the relationship between moe-phobia and conservative tendency was limited. These findings provide new insights into creating sexual advertisements and discussing moe-phobia.
Cortical Vein Involvement and Its Influence in a Cohort of Adolescents With Cerebral Venous Thrombosis
Authors: liu lu , Zhou chenxia , Jiang Huimin , Wei Huimin , Zhou Yifan , Wu Yan , Zhang Kaiyuan , Duan jiangang , Meng Ran , Zhou Chen , Ji Xunming 
Published: May 2023

Abstract
Background and Purpose Cortical vein thrombosis (CVT) is a rare form of cerebral venous thrombosis (CVST) in adolescent patients that has received little attention. We aimed to analyze the clinical and radiological features of adolescents with CVST and investigate the effects of CVT involvement.Methods Patients aged ≥ 10 to ≤ 18 years and diagnosed with CVST were identified at Xuanwu Hospital, Capital Medical University between January 2015 and August 2022 and divided into two groups according to the presence or absence of cortical vein involvement. Clinical features, radiological characteristics, and 12-month follow-up outcomes were compared between the two groups.Results Fifty-three adolescents, including 21 with CVT, were included (mean age: 15.2 ± 1.8 years; females, 54.7%). The CVT group was more likely to experience seizures (P = 0.028) and deterioration (28.6% vs. 6.2%, P = 0.047) during hospitalization than the non-CVT group. Poor short-term outcomes, based on the modified Rankin Scale (mRS) score at discharge, were more common in adolescents with CVT (P = 0.007). The proportions of patients showing edema (42.9% vs. 6.2%, P = 0.004) and mass effect (P = 0.015) were significantly higher in the CVT group. Recanalization was observed in 61.9% and 82.1% of the patients in the CVT and non-CVT groups, respectively, during the first imaging review (median, 22 days). After a 12-month follow-up, female adolescents had more frequent resident secondary headaches than male adolescents (52.9% vs. 12.5%; P = 0.014).Conclusions Cortical vein involvement in adolescents with CVST was associated with a higher risk of epilepsy at presentation, deterioration during hospitalization, edema, and mass effect on acute imaging, as well as worse short-term outcomes. Sex differences require consideration in etiological analyses and prolonged follow-ups.
Erasable and Field Programmable DNA Circuits Based on Configurable Logic Blocks
Authors: Xiao Xianjin , Liu Yizhou , Zhai Yuxuan , Hu Hao , Liao Yuheng , Liu Huan , Liu Xiao , He Jiachen , Wang Limei , Wang Hongxun , Li Longjie , Zhou Xiaoyu 
Published: May 2023

Abstract
DNA is commonly employed as a substrate for the building of artificial logic networks due to its excellent biocompatibility and programmability. Till now, DNA logic circuits have been rapidly evolving to accomplish advanced operations. Nonetheless, the process of creating DNA logic circuits according to personal needs (logical truth table) requires extensive knowledge on digital circuits. Moreover, even after the researchers endeavor to build a DNA circuit, it lacks field programmability and thereby being disposable and inconvenient. Herein, inspired by the Configurable Logic Block (CLB) paradigm in silicon digital circuits, we present the CLB-based field-programmable DNA circuit that uses clip strands as its operation-controlling signals. It substantially simplifies the construction of desired circuits by establishing the relationship between circuits and operation-controlling strands. Additionally, the field programmability enables users to realize diverse functions with limited hardware. We firstly constructed CLB-based basic logic gates (OR and AND), and effectively demonstrate their eras ability and field programmability. Furthermore, by simply adding the appropriate operation-controlling strands, we achieved multiple rounds of switch among 5 different logic operations on a single two-layer circuit. In addition, we successfully built a circuit to implement two fundamental binary calculators: half-adder and half-subtractor, proving that our design could imitate silicon-based binary circuits. Finally, we built a comprehensive CLB-based circuit that enabled multiple rounds of switch among 7 different logic operations including half-adding and half-subtracting. Overall, the CLB-based field-programmable circuit greatly streamlines the process to build DNA circuits and immensely enhances their practicability. We believe our design could be widely used in DNA logic networks due to its efficiency and convenience.
Pointless parts of completely regular frames
Authors: Ball Richard N. 
Published: May 2023

Abstract

(Completely regular) locales generalize (Tychonoff) spaces; indeed, the passage from a locale to its spatial sublocale is a well understood coreflection. But a locale also possesses an equally important pointless sublocale, and with morphisms suitably restricted, the passage from a locale to its pointless sublocale is also a coreflection. Our main theorem is that every locale can be uniquely represented as a subdirect product of its pointless and spatial parts, again with suitably restricted projections. We then exploit this representation by showing that any locale is determined by (what may be described as) the placement of its points in its pointless part
2020 Mathematics Subject Classification. Primary 06D22; Secondary 54C45, 54G12, 54G10.
Is low regional Hounsfield units (HU) of screw trajectory being one of the risk factors of hardware failure after posterior cervical fusions (PCFs) with instrumentation?
Authors: Chen Zejun , Lv M. B Guohua , Li Yunchao , Wang Xiaoxiao , He M. B Haoyu , Yuan M. B Hui , Pan M. M Changyu , Kuang M. M Lei , Zhang Ou 
Published: May 2023

Abstract
BACKGROUND CONTEXT: For patients with multilevel degenerative cervical myelopathy, laminectomy and posterior cervical fusions (PCFs) with instrumentation are widely accepted technique for symptom relief. However, hardware failure is not rare which results in neck pain or even permanent neurological leision. The prevalence of hardware-related complications of laminectomy and posterior cervical fusions (PCFs) with instrumentation are lack of in-depth study. PURPOSE: To investigate risk factors of hardware failure after laminectomy and posterior cervical fusions (PCFs) with instrumentation in patients with degenerative cervical myelopathy. STYDU DESIGN: single-center, retrospective study. METHODS: Fifty-six patients who underwent laminectomy and posterior cervical fusions (PCFs) with instrumentation between January 2019 and January 2021 were included in a single institution. Patients were divided into the hardware failure group (n = 14) and no hardware failure group (n = 42). The sex, age, screw density, end vertebra (C7 or T1), cervical sagittal alignment parameters (C2-C7 lordosis, C2-C7 sagittal vertical axis, T1 slope, CL correction), and regional Hounsfield units (HU) of screw trajectory, morbidity of osteoporosis were collected and compared between the two groups. RESULTS: The mean overall follow-up was 20.6 months (range, 12–30 months). There were no significant statistical differences in general information (age, gender, follow-up period) of patients between the two groups. The differences of fusion rate, fixation levels and screw density between the two groups were not statistically significant (p > 0.05). The failure rate of the fixation endpoint at T1 was lower than that at C7 (9% vs. 36.3%) (p < 0.05). The regional HU of pedicle screw(PS) and lateral mass screw(LMS) in failure group were lower than the ones of no failure group (PS: 267 ± 45 vs. 368 ± 43; LMS: 308 ± 53 vs. 412 ± 41) (p < 0.05). The sagittal alignment parameters did not show significant differences between the two groups before surgery and at final follow-up (p > 0.05). The hardware failure rate in cases without osteoporosis was lower than that in cases with osteoporosis (14.3% vs. 57.1%) (p < 0.05). CONCLUSION: Hardware failure was not uncommon after laminectomy and PCFs with instrumentation in patients with degenerative cervical myelopathy. Osteoporosis, fixation ended at C7, and low regional HU of screw trajectory were the independent risk factors.