bluuebunny/crossref_metadata_2025_split huggingface dataset:
A DNA Unwinding Equilibrium Serves as a Checkpoint for CRISPR-Cas12a Target Discrimination
Authors: Singh Jaideep , Liu Kevin G. , Allen Aleique , Jiang Wei , Qin Peter Z. 
Published: May 2023

ABSTRACTCRISPR-associated proteins such as Cas9 and Cas12a are programable RNA-guided nucleases that have emerged as powerful tools for genome manipulation and molecular diagnostics. However, these enzymes are prone to cleaving off-target sequences that contain mismatches between the RNA guide and DNA protospacer. In comparison to Cas9, Cas12a has demonstrated distinct sensitivity to protospacer-adjacent-motif (PAM) distal mismatches, and the molecular basis of Cas12a’s enhanced target discrimination is of great interest. In this study, we investigated the mechanism of Cas12a target recognition using a combination of site-directed spin labeling, fluorescent spectroscopy, and enzyme kinetics. With a fully matched RNA guide, the data revealed an inherent equilibrium between a DNA unwound state and a DNA-paired duplex-like state. Experiments with off-target RNA guides and pre-nicked DNA substrates identified the PAM-distal DNA unwinding equilibrium as a mismatch sensing checkpoint prior to the first step of DNA cleavage. The data sheds light on the distinct targeting mechanism of Cas12a and may better inform CRISPR based biotechnology developments.
Expression of transcription factors KLF2 and KLF4 is induced by the mammalian Golgi stress response
Authors: Sasaki Kanae , Sakamoto Miyu , Miyake Iona , Tanaka Reishi , Tanaka Ryuya , Tanaka Azusa , Terami Misaki , Komori Ryota , Taniguchi Mai , Wakabayashi Sadao , Sakurai Hajime Tajima , Yoshida Hiderou 
Published: May 2023

AbstractThe Golgi stress response is a homeostatic mechanism that augments Golgi function when Golgi function becomes insufficient (Golgi stress). Glycosylation of the core proteins of proteoglycans is one of the important functions of the Golgi. If the production of core proteins is increased and the amount of glycosylation enzymes for proteoglycans becomes insufficient (PG-type Golgi stress), the proteoglycan pathway of the Golgi stress response is activated, resulting in the transcriptional induction of glycosylation enzymes, including NDST2, HS6ST1 and GLCE. The transcriptional induction of these glycosylation enzymes is regulated by the enhancer element, PGSE-A; however, transcription factors that induce transcription from PGSE-A have not yet been identified. We herein proposed KLF2 and KLF4 as candidate transcription factors for transcriptional induction from PGSE-A, and revealed that their expression was up-regulated in response to PG-type Golgi stress. These results suggest that KLF2 and KLF4 are important regulators of the proteoglycan pathways of the mammalian Golgi stress response.
Brooding and neuroticism are strongly interrelated manifestations of the phenome of depression
Authors: Vasupanrajit Asara , Jirakran Ketsupar , Tunvirachaisakul Chavit , Maes Michael 
Published: May 2023

AbstractNeuroticism is a subclinical manifestation of the phenome of depression, comprising depressive and anxiety symptoms, and suicidal behaviors. Rumination is positively associated with depression and neuroticism and may mediate the effects of neuroticism on depression. This study aims to determine whether rumination or its components, including brooding or reflection, mediate the effects of neuroticism on depression, or alternatively, whether both neuroticism and rumination are manifestations of the phenome of depression. This study recruited 74 depressed subjects and 44 healthy controls. The depression group was split into groups with high versus low brooding scores. We used partial least squares (PLS) to examine mediation effects. We found that brooding and reflection scores are significantly higher in depressed patients than in controls. Patients with higher brooding scores have increased severity of depression, anxiety, insomnia, neuroticism, and current suicidal ideation as compared with patients with lower brooding scores and controls. There is a strong positive association between rumination, and neuroticism, depression, anxiety, and lifetime and current suicidal behaviors. PLS analysis shows that brooding does not mediate the effects of neuroticism on the depression phenome, because no discriminant validity could be established between neuroticism and brooding, or between neuroticism and brooding and the depression phenome. We were able to extract one validated latent vector from brooding and neuroticism, insomnia, depression, anxiety, and current suicidal behaviors. Overall, this study supports the theory that rumination and neuroticism are manifestations of the phenome of depression, just like affective symptoms, suicidal behaviors, and insomnia.
Moving forward through consensus: a national Delphi approach to determine the top research priorities in prostate cancer in Uganda
Authors: Ssemata Andrew Sentoogo , Muhumuza Richard , Seeley Janet , Lombe Dorothy Chilambe , Mwamba Monde , Msadabwe Susan , Mwaka Amos Deogratius , Aggarwal Ajay 
Published: May 2023

AbstractObjectiveTo describe the health system, socioeconomic and clinical factors that influence access to and outcomes of care for prostate cancer (PC) in Uganda.DesignQualitative Delphi techniqueSettingGovernment and private-not-for-profit hospitalsMethodsWe applied a two-stage modified Delphi technique to identify the consensus view across cancer experts. In the first round, experts received a questionnaire containing 22 statements drawn from a systematic review of the literature focusing on identifying the reason for the delay in accessing cancer care. Round 2 comprised 18 statements considered the greatest priority from round 1.ResultsWe found that the top five research priority areas arise from challenges including: (i) lack of Diagnostic services - Ultrasound, laboratory tests, and biopsy facilities; (ii) High costs of services e.g., surgery, radiotherapy, hormone therapy are unaffordable to most patients, (iii) Lack of critical surgical supplies and essential/critical medicines, (iv) Lack of awareness of cancer as a disease and low recognition of symptoms, (v) Low Healthcare literacy. The lack of critical surgical supplies, high costs of diagnostic investigations and treatments were ranked highest in order of importance in round one. Round two of the survey also revealed lack of diagnostic services, unavailability of critical medicines, lack of radiotherapy options, high costs of treatments and lack of critical surgical supplies as the top priorities.ConclusionThese research priority areas ought to be addressed in future research to improve prompt prostate cancer diagnosis and care in Uganda. There is need to improve the supply of high-quality affordable anticancer medicines provided timely to prostate cancer patients so as to improve the survivorship from the cancer.Article SummaryStrengths and limitations of this studyThis is a novel Delphi study exploring research priorities for prostate cancer research in Uganda from various stakeholders in cancer care.The study was informed by a recent systematic review which provided insight into the statements explored in the Delphi technique.Participants were recruited from various clinical, government aided and private settings and geographical locations giving representativeness of the participants.A limitation to this study was the low response rates in round 1 and round 2 as most stakeholders were not familiar with the Delphi technique, or how it works.
Overlapping representations of food and social stimuli in VTA dopamine neurons
Authors: Willmore Lindsay , Minerva Adelaide R. , Engelhard Ben , Murugan Malavika , McMannon Brenna , Oak Nirja , Thiberge Stephan Y. , Peña Catherine J. , Witten Ilana B. 
Published: May 2023

SummaryDopamine neurons of the ventral tegmental area (VTADA) respond to food and social stimuli and contribute to both forms of motivation. However, it is unclear if the same or different VTADAneurons encode these different stimuli. To address this question, we performed 2-photon calcium imaging in mice presented with food and conspecifics, and found statistically significant overlap in the populations responsive to both stimuli. Both hunger and opposite-sex social experience further increased the proportion of neurons that respond to both stimuli, implying that modifying motivation for one stimulus affects responses to both stimuli. In addition, single-nucleus RNA sequencing revealed significant co-expression of feeding- and social-hormone related genes in individual VTADAneurons. Taken together, our functional and transcriptional data suggest overlapping VTADApopulations underlie food and social motivation.
Assessing and predicting the quality of peer reviews: a text mining approach
Authors: Meng Jie 
Published: May 2023

PurposeThis paper aims to quantify the quality of peer reviews, evaluate them from different perspectives and develop a model to predict the review quality. In addition, this paper investigates effective features to distinguish the reviews' quality. Design/methodology/approachFirst, a fine-grained data set including peer review data, citations and review conformity scores was constructed. Second, metrics were proposed to evaluate the quality of peer reviews from three aspects. Third, five categories of features were proposed in terms of reviews, submissions and responses using natural language processing (NLP) techniques. Finally, different machine learning models were applied to predict the review quality, and feature analysis was performed to understand effective features.FindingsThe analysis results revealed that reviewers become more conservative and the review quality becomes worse over time in terms of these indicators. Among the three models, random forest model achieves the best performance on all three tasks. Sentiment polarity, review length, response length and readability are important factors that distinguish peer reviews’ quality, which can help meta-reviewers value more worthy reviews when making final decisions.Originality/valueThis study provides a new perspective for assessing review quality. Another originality of the research lies in the proposal of a novelty task that predict review quality. To address this task, a novel model was proposed which incorporated various of feature sets, thereby deepening the understanding of peer reviews.
A hostile work climate and workplace bullying: reciprocal effects and gender differences
Authors: Rosander Michael , Salin Denise 
Published: May 2023

PurposeIn this paper the authors argue that organizational climate and workplace bullying are connected, intertwined and affect each other. More precisely, the focus of the present study is how a hostile climate at work is related to workplace bullying. A hostile work climate is defined as an affective organizational climate permeated by distrust, suspicion and antagonism. The authors tested four hypotheses about the reciprocal effects and possible gender differences.Design/methodology/approachThe study is based on a longitudinal probability sample of the Swedish workforce (n = 1,095). Controlling for age, the authors used structural equation modelling and cross-lagged structural regression models to assess the reciprocal effects of a hostile work climate on workplace bullying. Gender was added as a moderator to test two of the hypotheses.FindingsThe results showed a strong reciprocal effect, meaning there were significant associations between a hostile work climate and subsequent bullying, β = 0.12, p = 0.007, and between baseline bullying and a subsequent hostile work climate, β = 0.15, p = 0.002. The forward association between a hostile work climate and bullying depended on gender, β = −0.23, p < 0.001.Originality/valueThe findings point to a possible vicious circle where a hostile work climate increases the risk of bullying, which in turn risks creating an even more hostile work climate. Furthermore, the findings point to gender differences in bullying, showing that the effect of a hostile work climate on workplace bullying was stronger for men.
Assessing the transition from mass production to lean manufacturing using a hybrid simulation model of a LEGO® automotive assembly line
Authors: Katsigiannis Michail , Pantelidakis Minas , Mykoniatis Konstantinos 
Published: May 2023

Purpose
With hybrid simulation techniques getting popular for systems improvement in multiple fields, this study aims to provide insight on the use of hybrid simulation to assess the effect of lean manufacturing (LM) techniques on manufacturing facilities and the transition of a mass production (MP) facility to incorporating LM techniques.

Design/methodology/approach
In this paper, the authors apply a hybrid simulation approach to improve an educational automotive assembly line and provide guidelines for implementing different LM techniques. Specifically, the authors describe the design, development, verification and validation of a hybrid discrete-event and agent-based simulation model of a LEGO® car assembly line to analyze, improve and assess the system’s performance. The simulation approach examines the base model (MP) and an alternative scenario (just-in-time [JIT] with Heijunka).

Findings
The hybrid simulation approach effectively models the facility. The alternative simulation scenario (implementing JIT and Heijunka LM techniques) improved all examined performance metrics. In more detail, the system’s lead time was reduced by 47.37%, the throughput increased by 5.99% and the work-in-progress for workstations decreased by up to 56.73%.

Originality/value
This novel hybrid simulation approach provides insight and can be potentially extrapolated to model other manufacturing facilities and evaluate transition scenarios from MP to LM.
Organizational maturity models: the leading research fields and opportunities for further studies
Authors: Kucińska-Landwójtowicz Aneta , Czabak-Górska Izabela Dagmara , Domingues Pedro , Sampaio Paulo , Ferradaz de Carvalho Carolina 
Published: May 2023

PurposeThe aim of the article is to determine research areas and to recognize the current direction in the development of maturity models, to indicate the key areas of organizational maturity models (OMMs) development and their classification as well as to pinpoint research gaps and areas of potential development of OMMs in the context of scientific research and the needs of management practitioners.Design/methodology/approachThe research was conducted using the literature review method, bibliometric analysis and visual mappings.FindingsThe empirical classification developed in this paper identified 12 categories based on management areas, constituting the criteria for classifying OMMs models, where OMMs are being developed: Information Technology, Project Management, Business Management and Strategy, Human Resource, Ergonomics, Health and Safety Management, Industry 4.0 concept, Knowledge Management, Process Management, Performance Management, Quality Management, Supply Chain Management, Risk Management and Innovation Management.Research limitations/implicationsThe main limitation is the analysis in the scope of topic OMMs including solely the Scopus and Thompson Reuters Web of Science database. Another shortcoming is conducting data analysis and classification based on the abstracts of the selected articles.Originality/valueThis work is a starting point to prospect trends for future revolving around the OMMs crossing different databases.
When does public debt hurt inequality in Africa?
Authors: Andoh Francis Kwaw , Attobrah Emmanuel , Opoku Alexander , Armah Mark Kojo , Dasmani Isaac 
Published: May 2023

PurposeThe question of what level of public debt can increase inequality has become crucial in Africa. In this study, the authors examine the effect of public debt on inequality in Africa and estimate the debt-inequality threshold. The authors then examine the moderating role of tax burdens and corruption in the relationship between public debt and inequality.Design/methodology/approachUsing data from the period 2005 to 2019 in 38 African countries, the generalized method of moment and the dynamic panel threshold regression techniques were employed to achieve the purpose of the study.FindingsThe results reveal that a 1% increase in public debt leads to a rise in inequality by about 0.17%. However, the effects doubles when the public debt ratio hits 57.47%. Tax burden worses the effect of public debt by about 2.9 percentage points, while control of corruption reduces debt effect on inequality by 61 percentage points.Research limitations/implicationsOwing to data availability, the study period was restricted to 2005 to 2019. Moreover, the study could not consider the disagreggation of inequality into different income groups due to pausty of data. While this could narrow the scope of the study, the results provide an important insight for policy makers.Originality/valueThis contributes to the scant literature on the effect of public debt on income inequality in African countries. This study is a novelty because its provides the level of public debt which worsens inequality in Africa, as well as the moderating effects of tax burden and corruption control.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-08-2022-0581
Adaptive friction compensation control of robotic pneumatic end-effector based on LuGre model
Authors: Dai Shijie , Li Shida , Ji Wenbin , Wang Ruiqin , Liu Shuyuan 
Published: May 2023

Purpose
Considering the response lag and viscous slip oscillation of the system caused by cylinder piston friction during automatic polishing of aero-engine blades by a robotic pneumatic end-effector, the purpose of this study is to propose a constant force control method with adaptive friction compensation.

Design/methodology/approach
First, the mathematical model of the pneumatic end-effector is established based on the continuous LuGre model, and the static parameters of the LuGre model are identified to verify the necessity of friction compensation. Second, aiming at the problems of difficult identification of dynamic parameters and unmeasurable internal states in the LuGre model, the parameter adaptive law and friction state observer are designed to estimate these parameters online. Finally, an adaptive friction compensation backstepping controller is designed to improve the response speed and polishing force control accuracy of the system.

Findings
Simulation and experimental results show that, compared with proportion integration differentiation, extended state observer-based active disturbance rejection controller and integral sliding mode controller, the proposed method can quickly and effectively suppress the polishing force fluctuation caused by nonlinear friction and significantly improve the blade quality.

Originality/value
The pneumatic force control method combining backstepping control with the friction adaptive compensation based on LuGre friction model is studied, which effectively suppresses the fluctuation of normal polishing force.
Media and ethical issues in terrorism reporting and the security implication in Nigeria
Authors: Bodunde Oluwafemi David , Layefa Goodluck Tamaramieye , Fasae Joseph Kehinde 
Published: May 2023

Purpose
This paper aims to investigate the relationship between media reporting and violent extremism to explain the ethical and security issues emanating from it in Nigeria.

Design/methodology/approach
This paper draws on security and ethical literature, while a multimethod approach consisting of in-depth and focus group interviews was used. Content analysis was also relied upon from the interviews granted by media experts on terrorism, security and ethics.

Findings
Findings revealed that journalists are facing problems in areas of freedom of expression, framing, content selection and personal security. Again, this paper opines that elements of public awareness and issues of relevance also push the media to excessive reporting in which ethics and security must stand to play a restraining role.

Research limitations/implications
The first limitation of this paper is the inability to interview some terrorists and know their reaction to mass media reporting on their activities because nobody is ready to own up that he is a terrorist and to point to an individual as a terrorist is a dangerous phenomenon. Again, not all reporters are ready for interviews because of the fact that they are not prepared for the academic exercise but rather for assignments that can yield fat money such as selling secret information to those who can buy them with huge amounts of money like the politicians.

Practical implications
There is a threat to life on both sides of the government and terrorists. Favoring one side in their report is an offense from another side. There is also a violation of their human rights in freedom of speech as a result of the political situation in Nigeria where the government is faced with insecurity that hinders media from freedom of the press to publish reports. Moreover, where the ethical issue is suppressed, it makes the government unpopular all over the world because of the lack of press freedom.

Originality/value
To the best of the authors’ knowledge, this paper is novel as being the first of its kind where media experts are involved in research attempts on media reporting and violent extremism in relation to security and ethical issues in Nigeria.
The nested and spillover effects of institutional environment on reporting standard convergence: a multilevel approach and an agglomeration economy perspective
Authors: Cao June 
Published: May 2023

PurposeThe objective of this study is to examine how the heterogeneity of the institutional environments within a single country influences International Financial Reporting Standards (IFRS) convergence and earnings quality based on a meso- and multi-level approach.Design/methodology/approachUsing hierarchical linear modeling (HLM) to capture the between-group heteroskedasticity and within-cluster interdependence, this study investigates the simultaneous effect by incorporating institutional factors residing at different hierarchical levels and the interaction effects of factors within the same level on IFRS convergence and earnings quality in the largest IFRS adopter, China.FindingsThe results show that after IFRS convergence (i.e. 2007–2015), earnings quality decreases in terms of conservatism. However, the further analysis indicates that the strong institutional environment could mitigate the negative impact of IFRS on conservatism.Originality/valueConsistent with the emphasis of heterogeneity within a country by Terraccianoet al.(Science, 2005, 310 (5745)), this study indicates that the heterogeneity in the institutional environments and the simultaneous effect of the multilevel institutional environments within a single country cannot be ignored. This study also indicates that, equally important, research methodology plays a substantial role in investigating the outcomes of IFRS convergence. Finally, this study, based on an integrated theory, adopts a meso-paradigm linking macro- and micro-level institutions to provide comprehensive insights into IFRS convergence and conservatism.