bluuebunny/crossref_metadata_2025_split huggingface dataset:
Native habitats and salt stress responses of wild Hydrangea macrophylla
Authors: Morimoto Hayato , Harada Senjiro , Shukuya Shogo , Yamamoto Sho , HANDA Takashi 
Published: June 2023

Abstract
Hydrangea shrubs, developed from wild Hydrangea macrophylla endemic to Japan, are very important plants for landscaping and horticulture. H. macrophylla, likely speciated from H. serrata growing in the mountainous zone, have migrated into coastal zone. However, there is still a lack of information about the habitats of wild H. macrophylla and its adaptative responses to salt-stressed coastal environments. Therefore, in this study we conducted a survey of wild H. macrophylla populations and its native habitats. The results of the habitat survey showed that H. macrophylla is mainly distributed in coastal zones that have a saline atmosphere, triggered by high wind speed, high soil EC1:5 and large soil sodium contents, capable of causing severe damage to many plant species. Additionally, we confirmed some inland populations in a part of the Izu Peninsula and on the Izu islands. Several of these inland H. macrophylla populations inhabited mountain vegetation areas favored by H. serrata, and had thinner leaves than the coastal populations. We also found H. macrophylla individuals growing in oligotrophic environments such as on rocky surfaces and in volcanic ash scoria. Leaf cation analysis suggests that H. macrophylla adapts to salt stress by regulating Na+ allocation and preventing high leaf Na+ concentrations. The findings of this fundamental investigation of native habitats and adaptation to coastal zone should help facilitate further studies on H. macrophylla ecosystem, abiotic stress and conservation.
Construction of a Competency Model for Occupational Health Post for Chinese Medical Students
Authors: Chen Lili , Zhao Jiyang , Chen Yangyang , Fu Xinyu , Zhang Tong , Jing Xueyan , Wu Yonghui , Wang Yue 
Published: June 2023

Abstract
Aims and Methods
To construct a scientific and reasonable competency model for occupational health post to provide a reference standard for the education and training of occupational health-related personnel. A purposive sampling method was used to select the investigation subjects. Thirty-seven experts from Prevention and Treatment Center for Occupational Diseases, Centre for Disease Control and Prevention, university research organization, occupational health services and Institute of Public Health Supervision in multiple locations were selected to develop a questionnaire for occupational health post competency through literature reading and semi-structured expert interview methods. Two rounds of consultation were conducted and SPSS 21.0 was applied for statistical analysis of the data.
Results
The occupational health post competency indication system with 5 primary indicators and 25 secondary indicators was determined. And the competency was stratified from inside to outside with reference to the "onion model", then the occupational health post competency model was completed.
Conclusion
The final constructed competency model for occupational health post was divided into three layers, with the innermost layer being professionalism, the middle layer being professional skills, general competence and work and development, and the outer layer being basic knowledge. The use of the model can be promoted to improve the post competency evaluation mechanism and clarify the positioning of occupational health-related personnel at different stages of their personal career development.
Characterization of necroptosis and immune landscape in dermatomyositis by bioinformatics analysis and machine learning
Authors: Dai Mengmeng , Zhang Chunyi , Li Ruxu , Gao Congcong , Yue Runzhi , Su Zhaohui , Li Chaoying , Zheng Zhaohui 
Published: June 2023

Abstract
BackgroundDermatomyositis (DM) is a cell-mediated autoimmune disease of intricate aetiology. Necroptosis is a newly identified form of programmed cell death. This research aimed to explore the value of necroptosis-related genes in DM.
Methods DM datasets were obtained from Gene Expression Omnibus (GEO) database. Necroptosis-related differentially expressed genes (NRDEGs) of DM were identified by intersecting differentially expressed genes (DEGs) with necroptosis gene set. Then, signature genes of NRDEGs were determined by the machine learning method of random forest (RF), support vector machine-recursive feature elimination (SVM-RFE), and the least absolute shrinkage and selection operator regression (LASSO). Moreover, immune microenvironment of DM and its correlation with signature genes were created to assess immune dysregulation. Besides, functional enrichment analysis, protein-protein interaction (PPI) co-expression network construction, transcription factor (TF)-miRNA network analysis were collectively performed on signature genes. In addition, the Mfuzz expression pattern clustering and functional enrichment based on the optimal signature was conducted.
Results A total of 2524 DEGs in GSE143323 were obtained, including BAX, BIRC3, JAK3, SPATA2L and TNFSF10. Through the intersection with necroptosis gene set, 28 NRDEGs were examined. Furthermore, five signature genes were identified via machine learning and were verified in GSE1551. In immune landscape evaluation, signature genes were positively correlated with most immunocytes, human leukocyte antigen (HLA) genes, and immune checkpoints. Among them, TNFSF10 was the best diagnostic signature of DM. The most highly associated module genes with TNFSF10 by Mfuzz expression pattern clustering mainly enriched in immunity and immunoregulation.
Conclusions Necroptosis occurs in DM, and is closely related to DM immune microenvironment, which merits further investigations in the necroptosis of DM pathogenesis.
Evaluation of the occurrence of endophthalmitis according to the antibiotic prophylaxis treatment in patients with intraocular foreign bodies.
Authors: Bernier Raphaël , Elbany Sandra , Rocher Antonin , Gilli Christelle , Imbeau Léa , Rochepeau Cédric , Burillon Carole 
Published: June 2023

Abstract
Aim
The aim of the present study was to describe the clinical presentation of patients with open globe injuries (OGI) due to intra ocular foreign bodies (IOFBs) and to evaluate the occurrence of endophthalmitis in these patients. Moreover, this study evaluated the role of antibiotic prophylaxis in the prevention of endophthalmitis associated to IOFBs.
Methods
This study is a retrospective analysis of the medical records of patients with IOFBs presenting to the Edouard Herriot Hospital, Lyon, France, between May 2018 and February 2023. Patients who were operated for the removal of an IOFB were included. The clinical and epidemiological characteristics were evaluated, as well as theoccurrence of endophthalmitis according to the treatments used.
Results
A total of 35 patients who were operated to remove the IOFB were included. In one patient, the foreign body could not be removed. The surgery was anterior for 7 (20.0%) and posterior for 28 (80.0%). The median time to removal was 2 [1-3] days.
A total of 6 (17.1%) patients had an endophthalmitis at diagnosis, these patients did not receive antibiotic prophylaxis treatment. Among the patients without endophthalmitis, all patients received a systemic and local antibiotic prophylaxis; 19 (65.5%) patients were treated using intraocular antibiotics (IOA). Independently to the antibiotic prophylaxis treatment received, no patient developed endophthalmitis.
Conclusion
In the present study, no patient developed endophthalmitis postoperatively; independently of the antibiotic treatment received. Systemic and local antibiotics should be routinely used in IOFBs treatment, and intravitreal injections should have a broad indication.
Pancreatic Insulinoma with a Rare Association of Gray Matter Heterotopia: A Case Report
Authors: Hashmi Aateqa , Sarwar Muhammad Zeeshan , Iftikhar Sadaf , Naqi Syed Asghar 
Published: June 2023

Abstract
Background: Insulinoma is a functioning pancreatic neoplasm reported with some rare associations, but gray matter heterotopia has never been reported as its association in literature yet.
Case Presentation: We report the case of a nineteen-year-old man, a diagnosed case of epilepsy, who presented with typical Whipple’s triad. Computerized tomography of abdomen revealed nodule in the head of pancreas raising suspicion of insulinoma. As a part of workup to rule out multiple endocrine neoplasia type 1, magnetic resonance imaging of brain was performed which showed incidental finding of two foci of gray matter heterotopia in left lateral ventricle: one nodular growth in frontal horn causing corrugated outline and the other nipple-like outpouching in sub-ependymal location near centrum semi-ovale. After localization of pancreatic lesion, organ preserving open enucleation of tumour was performed. Histopathology confirmed 1.6 x 1.4 x 1 cmgrade II well differentiated neuroendocrine tumour. Post-operative complication of pancreatico-cutaneous fistula arose for which pancreatic duct stent placement was done through endoscopic retrograde cholangio-pancreaticography. Leakage from wound persisted for which the wound was surgically re-explored to perform pancreatico-gastrostomy. Leakage resolved and patient was discharged within a week. Epilepsy correlating with his incidental finding of gray matter heterotopia responded well to anti-epileptic medication hence requiring no surgical intervention.
Conclusion: We report gray matter heterotopia as a rare association of insulinoma which has never been reported in literature yet.
Single-cell analysis of sex and gender differences in the human brain during development and disease
Authors: Zelco Aura , Joshi Anagha 
Published: June 2023

Abstract
Sex and gender differences in human brain are of interest to society and science as numerous processes are impacted by them, including brain development, behavior, and the occurrence, prevalence, and therapeutic response of diseases. By assembling publicly accessible data from the in utero to elderly age in healthy, Alzheimer’s disease and multiple sclerosis samples, we thoroughly analyzed SG changes through brain development and brain disorders at a single-cell level. We identified and characterised SG-biased genes in ten key types of brain cells across nine age and illness groups, by analysing a total of 419,885 single-nucleus RNA- sequencing samples from 161 human brains (72 females, 89 males). SG-biased genes were located mostly on the autosomes and showed some enrichment for Y chromosome but not X. Most SG-biased genes were unique to cell types and were enriched for cell type specific markers. Furthermore, SG-biased genes showed little overlap across age and disease groups. Despite a very low gene overlap, there was a high functional overlap for SG-biased genes across cell types as well as across age and disease. Female-biased genes were enriched for brain-related processes, and male-biased genes were enriched for metabolic pathways. Mitochondrial genes were consistently female-biased. Both male- and female-biased genes were highly enriched for androgen (not estrogen) response elements and interestingly thymosin targets were enriched consistently only in male-biased genes. Finally, we have created a web resource with full characterisation of SG-biased genes at different thresholds for the scientific community, to validate findings and generate further new hypotheses, available at https://joshiapps.cbu.uib.no/SRB app/.
Effects of modular new prone positioning tools in patients with acute respiratory distress syndrome due to COVID-19: A randomized controlled trial
Authors: Huang Dunbing , Ke Xiaohua , Song Wei , Zhong Yue , Li Yiting , Xu Mengyao , Wang Jiaqi , Yao Zizhe , Xiong Lize , Zhang Anren 
Published: June 2023

Abstract

Background Prone position (PP) ventilation has become an effective and simple treatment for acute respiratory distress syndrome (ARDS) due to COVID-19; however, prolonged prone position not only leads to patient discomfort, but also reduces patient compliance, and is prone to causing adverse events such as pressure injuries, pain, and dizziness. We aim to explore the effects of modular new prone positioning tools in patients with ARDS due to COVID-19. Methods 168 patients with ARDS due to COVID-19 were selected; however, 92 were later disqualified. 76 patients were randomly assigned to the observation group (n = 38) and the control group (n = 38). The observation group used modular new prone positioning tools to implement prone ventilation therapy while the control group used soft pillows to implement prone ventilation therapy. Comfort indicators (including time spent implementing PP, duration of PP, number of postural adjustments during PP, and duration time when first needing to adjust position), adverse events (including artificial airway kinking, shortness of breath, dizziness, and stress injury), and efficacy indicators (including intubation and mortality) were collected. The feeling of comfort, the occurrence of adverse events, and the efficacy of two groups of patients were also evaluated. Results The observation group had shorter time spent implementing PP(2.74 ± 0.86 vs. 4.64 ± 1.02, P < 0.001), longer duration of PP (14.02 ± 1.01 vs. 13.03 ± 0.66, P < 0.001), duration time when first needing to adjust position (59.89 ± 12.73 vs. 36.57 ± 8.69, P < 0.001), and lower number of postural adjustments during PP (11.03 ± 2.67 vs. 17.95 ± 2.58, P < 0.001) in comparison with the control group. No significant differences in intubation (9 vs. 11, P = 0.602) and mortality (4 vs. 6, P = 0.602) were found in both groups. However, in terms of adverse events, the observation group showed lower artificial airway kinking (5 vs. 23, P < 0.001), pain (7 vs. 21, P = 0.001), shortness of breath (2 vs. 9, P = 0.022), dizziness (0 vs. 5, P = 0.021), and stress injury (7 vs. 26, P < 0.001) than the control group. Conclusion Utilizing modular new prone position tools to implement prone ventilation therapy not only improves the efficiency of prone position execution and patient comfort, but also reduces the incidence of adverse events. However, it cannot change the intubation rate and mortality rate of patients.
Comparison of Applications of Aloe Vera, Honey, Saliva, and Ovalbumin on Collagen Formation in Wound Healing of Incised Skin in Rats
Authors: Tomo R. Wahyu Kartiko , Dachlan Ishandono , Dewajani Dewajani 
Published: June 2023

Background: Aloe vera, honey, human saliva, and ovalbumin are useful in the wound healing process. Collagen acts as an indicator of wound healing.Objective: This research aimed to compare the effect of Aloe vera, honey, human saliva and ovalbumin on collagen formation in wound healing.Design: This research was a laboratory experimental study on sixty white rats (Rattus novergicus) which were divided into 5 groups. Four groups were cases with skin applications and 1 group was controls with NaCl. We performed general anesthesia procedure, making an incision on skin of back, then the wound was sutured, and on surface of the sutured wound we applied substrate twice a day for six days. On day four and seven, after euthanasia, the sutures were removed, and the incised skin was taken for histological examination. We counted and analyzed the size of collagen with Image Raster tool programs. Data were analyzed with t-test, Mann Whitney, ANOVA and Kruskal Wallis tests, and then with post hoc tests.Results: After day four, the mean area of collagen of Aloe vera group was 55391.19 ± 30939.63, honey 24194.88 ± 43700.39, saliva 25869.86 ± 31608.39, ovalbumin 82459.19 ± 80994.66, NaCl 97658.04 ± 88452.74, with no significant differences between collagen sizes with p=0.125 (˃0.05). Decapitation day seven, significant differences with p=0.017 (˂0.05), in honey group 18601.67 ± 29260.28 and saliva 17560.31 ± 27262.03 than the control NaCl 94126.22 ± 29500.27.Conclusions: Collagen sizes after day four among the groups of Aloe vera, honey, saliva, ovalbumin and saline did not differ significantly. Collagen sizes after day seven of honey and saliva groups were significantly different than the controls.
Unit Cost Calculation for Low Calorie Diet Packages to Support the Nutrition Installation Entrepreneurship of UGM Academic Hospital
Authors: Sari Pratiwi Dinia 
Published: June 2023

Background: Nowadays, people have the awareness to maintain weight and to eat healthy diet. However, people prioritize practicality in preparing food due to the limited ability to prepare healthy food. Nutrition Installation in Universitas Gadjah Mada Academic Hospital has the potential to become a profit center by developing an entrepreneurship sector, specifically by providing a healthy catering services. To organize a good healthy catering services, preparations need to be done, including those related to human resources, equipment, operating systems, and to-be-applied tariffs. Objective: The purpose of this study was to determine the unit cost of the low-calorie diet package, the product of the entrepreneurship sector of Nutrition Installation. Design: Descriptive research with quantitive approach. This research was held at the Nutrition Installation in UGM Academic Hospital from June to November 2019, with the research steps conducted: 1) Identifying the details of the low-calorie diet package, 2) Identifying the direct cost consisting of direct non-overhead cost and direct overhead cost, 3) Identifying the indirect cost, 4) Calculating the unit cost. Results: The unit cost for a low-calorie diet package is IDR 70.553 per portion consisting of lunch, snack, assessment, and nutritional consultations per package. Conclusions: The unit cost obtained from this study can be used as the basis to determine the tariff for the hospital’s Nutrition Installation’s healthy catering services.
Insight of PrEP and Testing STI Puzzle
Authors: Rusetiyanti Nurwestu 
Published: June 2023

Pre Exposure Prophylaxis (PrEP) is the use of Anti Retroviral (ARV) drugs by HIV-uninfected people to block the acquisition of HIV before exposure to HIV. There are concerns that PrEP introduction and scale up may pose risks, such as sexual behavior change in specific populations, and have an impact on the budget in already constrained health systems. Therefore, public health measures for the prevention of HIV and other STIs need to be enhanced, such as the prioritization of PrEP, coupled with more effective STI screening and treatment. Integration of STI and PrEP programmes can be viewed bi-directionally (not only integrating STI services into PrEP services but also considering STI clients as people also at risk for HIV and therefore potentially eligible for PrEP). This approach fosters synergies and efficiencies from a public health perspective. However, there are many challenges to programmatic integration, including siloed funding streams and programmes, the availability and costs of expanded etiological STI testing, and gaps in capacity and training for STI management. Keywords : PrEP, STI, services
The Influence Of Wearing Lumbo Sacral Orthosis Against The Strength Of Trunk Muscles In Case Of Low Back Pain (LBP) E.C Spondylolisthesis
Authors: Setiyawan Sholeh Setiyawan 
Published: June 2023

Background: About 80-90% of patients LBP stated that they did not do any attempts to treat his illness. Spondylolisthesis (spon-dee-lo-lis-thee-sis) is a condition where one of the spinal vertebrae to shift forward or backward with the next vertebra. The disruption that can be caused due to this condition include a painful tap on regio lumbar muscle spasm, occurs a decrease in muscle strength, limitation of motion, can also happen pain in the limbs. The purpose of this research is to know the influence of the use of the Lumbo Sacral Orthosis semirigid type to the strength of Trunk muscles in case of Low Back Pain (LBP) e. c Spondylolisthesis.Subjects and method: this type of research was quasi-experimental design pre-test and post-test design. Location of the study Area General Hospital in Yogyakarta, Gadjah Mada University Academic Hospital, Dr. Soeradji Tirtonegoro Hospital, Tugurejo Semarang Hospital. The samples in this research are 35 patients with purposive sampling.Results : there was the influence of the use of the Semirigid Type LSO against muscle flexion trunk before and after treatment (Z =-5.099 with p value = 0.000), trunk rotation muscle (Z =-4.600 with p value = 0.000), trunk extension muscle strength (Z =-4.472 with p value = 0.000).Conclusion: As for the results of this research are the greatest influence has decreased muscle strength of the trunk after wearing the LSO within one month is on the muscle group Flexor (0.75), then extensor muscles (0.58) and rotator trunk muscles (0.65).Keywords: Lumbo Sacral Orthosis semirigid type, The strength of Trunk muscles, Low Back Pain (LBP) e. c Spondylolisthesis.
The Impact of Reuse Dialyzer Application on Routine Hemodialysis Patients Adequacy and Its Correlation with Cost Efficiency in Type D Hospital
Authors: Ningtiyas Rulita Ayu Kusuma 
Published: June 2023

Background: Hemodialysis (HD) is one of the high cost service unit in the hospital which it implemented the cost effciency policy by the application of reuse dialyzer. According to claim recieved from Jaminan Kesehatan Nasional (JKN) system, the HD procedure for type D hospitals received the smallest claim payment if it compared to type A, B and C hospitals. This policy will be measured its effect on the effectiveness in the form of Ureum Reduction Ratio (URR) and Kt/V values and it associated with cost efficiency in type D hospital settings. Objectives The aims of this study were to 1.) Measure the corellation between the policy of using reuse dialyzer and the HD adequacy value and 2.) Evaluate the cost efficiency policy implemented by type D hospital HD unit in the form of using reuse dialyzer. Methods: This study was conducted with a cross-sectional design study from URR and Kt/V data on routine HD patients at 4 of type D hospital in Yogyakarta and Cantral Java in period Januari-March 2021. HD adequacy values were compared between the groups using the new and reuse dialyser. Cost efficiency was calculated based on the cost difference between cost of the HD procedure using a reuse dialyser and the projected cost if it is carried out with a single-use dialyzer.Result and Discussion: HD adequacy data were obtained from 112 subjects consisting of 22 with a new dialyzer, 32 with a reused (R) dialyzer first and R2, 27 with R3-R4 and 31 with R5-R7.The analysis of the effect of using reuse dializer on URR and Kt/V in the group with the new and reuse dialyser was obtained p = 0,90 and 0,91. The HD adequacy value in using reuse dialyser was not significantly different from using the new dialyser. The result of the cost efficiency analysis was 20.55% with using reuse dialyzer when it is associated with a source of income for HD services in type D hospitals.Conclusion: The application of reuse dialyzer is proved that it did not affect HD adequacy achievement when it compared to the use of a new dialyzer, and saved costs on HD services in type D hospitals. Keywords: Hemodialysis, reuse dialyzer, HD adequacy, efficiency