bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anisotropically Epitaxial P‐N Heterostructures Actuating Efficient Z‐Scheme Photocatalytic Water Splitting
Authors: Lin Haifeng , Kong Mengdan , Zou Zhitao , Li Xue , Wang Hui , Xu Jixiang , Turkevich Vladimir , Li Yanyan , Wang Xun , Wang Lei 
Published: January 2025

AbstractCrafting anisotropically epitaxial p‐n heterostructures with Z‐scheme charge transmission is a promising avenue toward excellent photocatalytic efficiency, yet the large lattice mismatch and diverse crystal growth habits between components have often arisen as a big challenge to this goal. Here, anisotropically epitaxial p‐n heterostructures with 19.8% lattice mismatch are obtained via a dynamics‐mediated seeded growth tactic under reaction temperature as low as 60 °C. Structural analyses reveal the epitaxy of hexagonal CuS nanoplates onto CdS nanowires through forming misfit dislocations at {101̄0} interface and stacking faults inside CuS nanoplates. Experimental and density functional theory calculation results verify the Z‐scheme photo‐carriers transfer in epitaxial CdS‐CuS heterostructures, which exhibit a much enhanced visible‐light‐driven H2 generation capability than non‐epitaxial CdS/CuS counterpart, and the site‐specified NiOOH photo‐deposition over CdS‐CuS heterostructures leads to a distinguished H2‐evolving activity ≈65 and 36 times promotion compared to those of pristine CdS and Pt‐loaded (3 wt.%) CdS, respectively. The study can enlighten new thinking to the steerable synthesis of epitaxial nanostructures with large lattice mismatch for various promising applications.
Advanced Functional NiCo2S4@CoMo2S4 Heterojunction Couple as Electrode for Hydrogen Production via Energy‐Saving Urea Oxidation
Authors: Nwaji Njemuwa , Fikadu Boka , Osial Magdalena , Warczak Magdalena , Moazzami Goudarzi Zahra , Gniadek Marianna , Asgaran Sohrab , Lee Jaebeom , Giersig Michael 
Published: January 2025

AbstractThe urea oxidation reaction (UOR) is characterized by a lower overpotential compared to the oxygen evolution reaction (OER) during electrolysis, which facilitates the hydrogen evolution reaction (HER) at the cathode. Charge distribution, which can be modulated by the introduction of a heterostructure, plays a key role in enhancing the adsorption and cleavage of chemical groups within urea molecules. Herein, a facile all‐room temperature synthesis of functional heterojunction NiCo2S4/CoMo2S4 grown on carbon cloth (CC) is presented, and the as‐prepared electrode served as a catalyst for simultaneous hydrogen evolution and urea oxidation reaction. The Density Functional Theory (DFT) study reveals spontaneous transfer of charge at the heterointerface of NiCo2S4/CoMo2S4, which triggers the formation of localized electrophilic/nucleophilic regions and facilitates the adsorption of electron donating/electron withdrawing group in urea molecules during the UOR. The NiCo2S4/CoMo2S4// NiCo2S4/CoMo2S4 electrode pair required only a cell voltage of 1.17 and 1.18 V to deliver a current density of 10 and 100 mA cm−2 respectively in urea electrolysis cell and display very good stability. Tests performed in real urine samples show similar catalytic performance to urea electrolytes, making the work one of the best transition metal‐based catalysts for UOR applications, promising both efficient hydrogen production and urea decomposition.
Greenness Assessment of High‐Performance Liquid Chromatography Method for Simultaneous Estimation of Apigenin, Apocynin, and Gallic Acid With Quality by Design Approach
Authors: Mevada Shweta , Shukla Saurabh , U Patel Harsha 
Published: January 2025

ABSTRACTThis study presents the development of an eco‐friendly high‐performance liquid chromatography (HPLC) method for the simultaneous determination of apigenin, apocynin, and gallic acid, integrating analytical quality by design (AQbD) and green analytical chemistry (GAC) principles. The objective was to enhance method efficiency while reducing environmental impact, energy consumption, and solvent use. An AQbD approach was employed, starting with quality risk assessment and scouting analysis, followed by optimization using Box–Behnken design (BBD) to screen three key chromatographic parameters. The optimal chromatographic conditions were determined using a C18 column (250 mm × 4.6 mm, 5 µm) with a mobile phase of methanol: water (80%:20% v/v), a flow rate of 1 mL/min, ultraviolet detection at 228 nm, and a temperature of 25°C. The method demonstrated linearity over a concentration range of 1–5 µg/mL for the target compounds, with gallic acid as an internal standard. The method's greenness profile was assessed using various evaluation tools, confirming its compliance with GAC principles. The results indicate that the proposed HPLC method is not only effective and reliable but also environmentally sustainable, making it suitable for routine use in quality control laboratories. The integration of AQbD and GAC principles highlights the method's contribution to more sustainable and efficient analytical practices.
Potential Socioeconomic Impacts of the Grand Ethiopian Renaissance Dam Operation on Inland Fisheries in the High Aswan Dam Reservoir
Authors: Alsadeq Mai , Nassar Yasmin , Eldayem Ahmed Wagdy Abd 
Published: January 2025

ABSTRACTThe High Aswan Dam (HAD) reservoir is considered as the most vital reservoir in Egypt and as a source of freshwater fish. It contributes 69% of the total inland fisheries production. The annual fish production from the HAD reservoir is estimated by the General Authority for Fishery Resources Development (GAFRD) as 12,000 ton/year. The HAD reservoir is the main source of freshwater fisheries in Egypt. That amount of fish in the reservoir guarantees a constant source of income and employment since the HAD establishment. Fish production is significantly affected by three environmental key parameters: water level fluctuation, water temperature change, and sedimentation amount. Any alteration in these three parameters will significantly affect the social and economic status of the HAD reservoir fisheries. This study aims to quantify the potential socioeconomic consequences of the Grand Ethiopian Renaissance Dam (GERD) operation on the HAD reservoir fishery. To quantify these potential impacts, this research utilizes pre‐GERD socioeconomic data gathered by national and international organizations such as WorldFish (https://worldfishcenter.org/) and GAFRD, to establish baseline data. The change in predicted socioeconomic assessment is carried out based on the water level, temperature change, and sedimentation, after the GERD operation. A significant reduction in fish production is anticipated, with an estimated average decline by 48.5% compared to current production rates. This decline is expected to lead to a decrease in the overall socioeconomic value of the HAD reservoir fishery, potentially resulting in job losses for 25% to 72% of the fishermen and distributors employees. The total fishing income is expected to decrease, because of the decrease in fish production. The research findings highlight the importance of policy considerations to mitigate the possible socioeconomic impacts. Potential policy interventions could include stricter fishing regulations, alternative livelihood programs for affected communities, and collaborative management strategies with upstream dam operators.
Prognostic significance of the mEPE score in intermediate-risk prostate cancer patients undergoing ultrahypofractionated robotic SBRT
Authors: Mose Lucas , Loebelenz Laura Isabel , Althaus Alexander , Ahmadsei Maiwand , Mathier Etienne , Broemel Isabelle , Aebersold Daniel M. , Obmann Verena Carola , Shelan Mohamed 
Published: January 2025

Abstract

Purpose
This study aimed to evaluate the prognostic significance of magnetic resonance imaging (MRI) parameters on biochemical failure-free survival (BFS) in patients diagnosed with intermediate-risk prostate cancer and treated with robotic ultrahypofractionated stereotactic body radiotherapy (SBRT) without androgen deprivation therapy (ADT).

Methods
A retrospective analysis was conducted in patients with intermediate-risk prostate cancer undergoing robotic SBRT delivered in five fractions with a total radiation dose of 35–36.25 Gy. The primary endpoint was biochemical failure as defined by the Phoenix criteria. Among other clinicopathological data, T stage, Prostate Imaging-Reporting and Data System (PI-RADS) score, and multiparametric magnetic resonance imaging-based extra-prostatic extension (mEPE) score were collected and analyzed using the log-rank test.

Results
A total of 74 patients were eligible for analysis. Median age at treatment was 68.8 years and median prostate volume was 47.8 cm3. Fifty-four and 14 patients were diagnosed with Gleason scores 7a and 7b, respectively. In total, 40 patients were classified as having unfavorable intermediate-risk prostate cancer according to American Urological Association/American Society for Radiation Oncology/ Society of Urologic Oncology (AUA/ASTRO/SUO) guidelines. The median follow-up was 30 months (range: 4–91.2 months; interquartile range (IQR): 18.5–48 months). The 3‑year BFS was 92%. A total of 12 (16.2%) biochemical failures were reported. In univariate analysis, an mEPE score of 5, the delivered total radiation dose (35 Gy vs. 36.25 Gy), and a prostate-specific antigen (PSA) nadir >1 ng/ml were associated with lower BFS (mEPE–BFS: p < 0.001, total radiation dose–BFS: p = 0.04, PSA nadir–BFS: p =< 0.001).

Conclusion
Patients diagnosed with intermediate-risk prostate cancer with a high mEPE score are more likely to experience biochemical failure after SBRT. Treatment intensification measures, such as administration of concomitant ADT, should be considered.
Bakterielle Vaginose – ein Syndrom zwischen gestern und heute
Authors: Swidsinski Alexander , Swidsinski Sonja , Mendling Werner , Farr Alex , Dörffel Yvonne 
Published: January 2025

Zusammenfassung
Ribosomale FISH(Fluoreszenz-in-situ-Hybridisierung)-Analysen zeigen, dass die bakterielle Vaginose (BV) ein komplexes klinisches Syndrom ist, das mehrere, nicht miteinander verwandte Infektionsprozesse mit ähnlicher Symptomatik umfasst. Die Biofilm-Vaginose ist die häufigste Ursache des BV-Syndroms, gefolgt von der kohäsiven Lactobacillus-iners-Sludge-Vaginose. Zum BV-Syndrom gehören darüber hinaus bisher nicht eindeutig charakterisierte Dysbiosen, wobei nicht ausgeschlossen ist, dass diese später in weitere nosologisch klar definierte Gruppen unterteilt werden. Derzeit lassen sich pathogene Konsortien nur mittels ribosomaler FISH bestimmen. Weder Lichtmikroskopie noch Multiplex-PCR (Polymerasekettenreaktion) und NGS („next generation sequencing“) sind hierfür hilfreich, da sie lediglich beteiligte Morphotypen bzw. Spezies, nicht aber ihre Wechselbeziehungen aufzeigen. Da alle polymikrobiellen Gemeinschaften auf spezielle Gene angewiesen sind, die für den Zusammenhalt der beteiligten Mikroorganismen notwendig sind, ist es nur eine Frage der Zeit, bis diese DNA-Sequenzen aufgedeckt und ihr Nachweis für die klinische Diagnostik und Therapie zugänglich sind.
Single-cell transcriptomics in a child with coenzyme Q10 nephropathy: potential of single-cell RNA sequencing in pediatric kidney disease
Authors: Park Peong Gang , Choi Sowon , Ahn Yo Han , Kim Seong Heon , Kim Chaeyoon , Kim Hyun Je , Kang Hee Gyung 
Published: January 2025

Abstract

Background
Coenzyme Q10 (CoQ10) nephropathy is a well-known cause of hereditary steroid-resistant nephrotic syndrome, primarily impacting podocytes. This study aimed to elucidate variations in individual cell-level gene expression in CoQ10 nephropathy using single-cell transcriptomics.

Methods
We conducted single-cell sequencing of a kidney biopsy specimen from a 5-year-old boy diagnosed with a CoQ10 nephropathy caused by a compound heterozygous COQ2 mutation complicated with immune complex-mediated glomerulonephritis. The analysis focused on the proportion of cell types, differentially expressed genes in each cell type, and changes in gene expression related to mitochondrial function and oxidative phosphorylation (OXPHOS).

Results
Our findings revealed a uniform downregulation of mitochondrial gene expression across various cell types in the context of these mutations. Notably, there was a specific decrease in mitochondrial gene expression across all cell types. The study also highlighted an altered immune cell population proportion attributed to the COQ2 gene mutation. Pathway analysis indicated a downregulation in OXPHOS and an upregulation of various synthesis pathways, particularly in podocytes.

Conclusions
This study improves our understanding of CoQ10 nephropathy’s pathogenesis and highlights the potential applications of single-cell sequencing in pediatric hereditary kidney diseases.

Graphical Abstract
Weighing up the potential of “superfoods” compounds of green tea or turmeric as adjuncts in comparison to established therapeutical approaches for periodontal disease
Authors: Heuzeroth Georg , Kaufmann Manuela Elena , Herter-Aeberli Isabelle , Schlagenauf Ulrich , Liu Chun Ching , Papageorgiou Spyridon N. , Schmidlin Patrick R. 
Published: January 2025

Abstract

Objective
Aim of this study was to critically appraise clinical evidence on the potential benefits of adjunctive use of superfoods green tea and turmeric as mouthrinse or local delivery agents in the treatment of periodontal disease.

Materials and methods
Electronic searches were performed in four databases for randomized trials from inception to February 2024 assessing the supplemental use of superfoods green tea and turmeric for gingivitis/periodontitis treatment. After duplicate study selection, data extraction, and risk-of-bias assessment with the RoB 2 tool, random-effects meta-analyses of Mean Differences (MD) or Standardized Mean Differences (SMD) with their 95% confidence intervals (CI) were performed.

Results
Nineteen studies (814 patients) were included, with 11 on gingivitis and 8 on periodontitis patients. No benefits were seen from the use of mouthwashes containing green tea extract or turmeric for gingivitis treatment, while green tea was associated with smaller Gingival Index (GI) reductions than chlorhexidine (5 studies; MD = 0.08; 95% CI = 0.01 to 0.14; P = 0.01). As far as periodontitis treatment is concerned, local supplementation with turmeric showed no benefits, whereas local supplementation with green tea extract was associated with improved treatment outcomes in terms of probing depth (4 studies; MD=-0.79; 95% CI=-1.29 to -0.29 mm; P = 0.002) and GI (3 studies; MD=-0.53; 95% CI=-1.01 to -0.05; P = 0.02) than the control group. However, the strength of evidence was moderate to very low due to bias, imprecision, and inconsistency.

Conclusion
Limited evidence indicates that supplemental use of green tea extract is associated with improved periodontal treatment outcomes. However, the strength of evidence is weak and further research is needed.

Clinical relevance
Green tea extract could be a natural adjunct to enhance periodontal treatment, without the potential side-effects of other adjuncts like chlorhexidine.
Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro
Authors: dos Santos Sanches Natália , Panahipour Layla , Wang Lei , Imani Atefe , Marchiolli Caroline Liberato , Cervantes Lara Cristina Cunha , Stein Maria Cristina Ruiz Voms , Berton Sara Alves , Souza Francisley Ávila , Okamoto Roberta , Júnior Idelmo Rangel Garcia , Gruber Reinhard 
Published: January 2025

Abstract

Objective
Titanium surface modifications improve osseointegration in dental and orthopedic implants. However, soft tissue cells can also reach the implant surface in immediate loading protocols. While previous research focused on osteogenic cells, the early response of soft tissue cells still needs to be better understood.

Material and methods
We have established a bioassay to this aim where human gingival fibroblasts, HSC2 oral squamous carcinoma cells, and murine bone marrow cells were cultured onto titanium discs or exposed to the respective supernatants for overnight. Modifications were double acid-etching (SLA), and coating with simulated body fluid (SBF) with or without odanacatib (ODN), a selective cathepsin K inhibitor reducing bone resorption.

Results
Our findings indicate that direct contact with titanium discs, with all surface modifications, slightly reduces cell viability. Growing gingival fibroblasts on discs consistently showed a trend toward increased IL8 expression. In HSC2 cells, this setting significantly increased IL1 and IL8 expression, confirmed by the immunoassay. Murine bone marrow macrophages also showed an increase in IL1 and IL6 expressions. Supernatants of the respective discs failed to cause these changes. Although ODN coating inhibited cathepsin K, osteoclastogenesis remained unchanged.

Conclusions
These findings suggest that titanium discs do not provide a favorable in vitro surface for oral soft tissue cells as they lose viability and respond with a moderately increased expression of inflammatory cytokines.

Clinical relevance
The soft tissue surrounding a dental implant can impact rehabilitation success. Understanding how soft tissue cells respond to titanium surface is potentially relevant to understand clinical outcomes.
Associated diseases and their effects on disease course in patients with chronic non-bacterial osteomyelitis: retrospective experience from a single center
Authors: Öksüz Aydın Betül , Aydın Fatma , Taş Özen , Bahçeci Onur , Özçakar Zeynep Birsin 
Published: January 2025

Abstract

Objective
Chronic non-bacterial osteomyelitis (CNO) is a rare autoinflammatory bone disease associated with other chronic inflammatory diseases such as familial Mediterranean fever (FMF), juvenile idiopathic arthritis (JIA), spondylarthropathies, inflammatory bowel disease (IBD), and pyoderma gangrenosum. We aimed to describe the clinical and follow-up characteristics of patients with CNO and to compare findings between patients with and without comorbidities.

Methods
The clinical records of patients with CNO who were followed up in our pediatric rheumatology clinic between 2018 and 2023 were reviewed. Patients were divided into two groups according to the presence or absence of comorbidities. The clinical, laboratory, and radiological characteristics and treatments of the groups were compared.

Results
The study included 40 patients (65% male) diagnosed with CNO. The median (IQR) age at symptom onset was 10 (6.4) and at diagnosis was 11.5 (5.9) years. Fourteen (35%) patients had comorbidities. The comorbidities were FMF (n = 9), IBD (n = 3), uveitis (n = 3), psoriasis (n = 1) and acne conglabatae (n = 2). The group with comorbidities had higher number of bones involved (3 or more bones) (78.6% versus 42.3%) (p = 0.028), and 78.6% of patients with comorbidities received biologic treatment, while only 23.1% of patients without comorbidities were treated with biologics (p = 0.001).

Conclusion
Familial Mediterranean fever, uveitis, IBD, psoriasis and acne conglabata were found to be the clinical conditions associated with CNO. Patients with CNO who had comorbidities appeared to have a more severe phenotype of the disease accompanied with more bone involvement and requiring more biologic treatment.

Key Points• Chronic non-bacterial osteomyelitis (CNO), an auto-inflammatory bone disease, can be seen in association with other inflammatory conditions such as familial Mediterranean fever (FMF), uveitis, inflammatory bowel disease (IBD), psoriasis, and acne conglobata.• If CNO is associated with another inflammatory disease, the number of bones involved may be higher and patients may need more intensive treatments, such as biologics.• CNO may coexist with one or more inflammatory diseases, which may exacerbate the disease phenotype.
Identification of plant-based spilled oils using direct analysis in real-time–time-of-flight mass spectrometry with hydrophobic paper sampling
Authors: McCallum Paige , Saturos Genesis , Rabinovitch Lola , Filewood Taylor , Kwok Honoria , Yan Jeffrey , Cody Robert , Brunswick Pamela , Shang Dayue 
Published: January 2025

Abstract
Spilled plant-based oils behave very differently in comparison to petroleum oils and require different clean-up measures. They do not evaporate, disperse, dissolve, or emulsify to a significant degree but can polymerize and form an impermeable cap on sediment, smothering benthic media and resulting in an immediate impact on the wildlife community. The current study explored the application of rapid up-to-date direct analysis in real time (DART) with high-resolution mass spectrometry for plant-based oil typing. The study introduced a new concept of using hydrophobic paper to collect and analyze oil samples, thus minimizing sample preparation and expenses. Application of this technique showed its ability to speedily distinguish plant-based from petroleum-based oils. A microcosm experiment exposing plant-based oil samples to weathering processes for comparison with petroleum-based oils demonstrated the ability of the method to classify weathered oil samples and identify their source oil. It was observed that canola and peanut oil were the most resistant to weathering processes. The developed DART-TOFMS method was shown to be accurate for short-term weathered oil spills up to between 12 and 26 days of exposure. The developed method performed identification in less than a day compared to the established multi-day method for oil spill forensics requiring careful sample collection in glass containers, time-consuming laboratory clean-up, lengthy gas chromatography sequences, and careful integration including integration of retention time markers.
Root productivity contributes to carbon storage and surface elevation adjustments in coastal wetlands
Authors: Conroy Brooke M. , Kelleway Jeffrey J. , Rogers Kerrylee 
Published: January 2025

Abstract

Background and aims
Organic matter additions in coastal wetlands contribute to blue carbon sequestration and adjustment to sea-level rise through vertical substrate growth, with accurate modelling of these dynamics requiring information of root mass and volume additions across tidal gradients. This study aims to characterise the influence of vegetation zonation and tidal position on root mass and volume dynamics within substrates.

Methods
The root ingrowth technique was coupled with sediment cores to quantify below-ground root mass and volume production, standing stocks and turnover across two years to 90 cm depth at Kooweerup, Victoria, Australia.

Results
We indicate a complex non-linear relationship between fine root mass production and tidal position, influenced by variable vegetation structures across mangrove (442–3427 g m−2 yr−1), saltmarsh (540–860 g m−2 yr−1) and supratidal forest (599 g m−2 yr−1) zones. Fine root volume additions ranged from 274 to 4055 cm3 m−2 yr−1 across sampling locations. Root production was greatest for older mangroves and tidally defined optimal zones of production were evident for mangrove and saltmarsh. Live roots extended deeper than typically studied, reaching depths of 1.0 m in forested zones.

Conclusion
This information of root mass and volume additions across wetland live rooting zones can be used to improve highly parameterised models accounting for carbon sequestration and substrate vertical adjustment along intertidal gradients. We recommend that future studies measure root production across the entire active rooting zone or to 1 m depth to align with standard carbon accounting measurement depths.