bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-invasive Phenotyping for Water and Nitrogen Uptake by Deep Roots Explored using Machine Learning
Authors: Changdar Satyasaran , Popovic Olga , Wacker Tomke Susanne , Markussen Bo , Dam Erik Bjørnager , Thorup-Kristensen Kristian 
Published: March 2023

Abstract
Background and aims

Root distribution over the soil profile is important for crop resource uptake. Using machine learning (ML), this study investigated whether measured planar root length density (pRLD) at different soil depths were related to uptake of isotope tracer (15N) and drought stress indicator (13C) in wheat, to reveal root function.

Methods

In the RadiMax semi-field root-screening facility 95/120 different winter wheat genotypes were phenotyped for root growth in 2018/2019, respectively. Using the minirhizotron technique, root images were acquired across a depth range from 80 to 250 cm in May, June, and July and pRLD was extracted using a convolutional neural network. We developed ML models to explore whether the pRLD estimates at different soil depths were predictive of the uptake of deep soil nitrogen - using deep placement of 15N tracer as well as drought resilience potential using natural abundance of 13C isotope. We analyzed the correlations to tracer levels to both an analytical root depth estimation and an ML approach. We further analyzed the genotypic effects on root function using mediation analysis.

Results

Both analytical and ML models demonstrated clear correlations between pRLD distribution and resource uptake. Further, both models demonstrated that deep roots at approx. 150 to 170 cm depth were most important for explaining the plant content of 15N and 13C isotopes. The correlations were higher in 2018 than in 2019.

Conclusions

The results demonstrated that in the semi-field non-invasive root phenotyping setup, analytical and ML-based analysis provided complementary insight into the importance of deep rooting for water and nitrogen uptake.
Modified honey related synthetic peptides in treating glioblastoma U-87 MG nude Mice Model, leukemia C1498 and lung carcinoma LLC syngeneic models
Authors: Elawdan Khaled M.E , Elawdan Youssof K. , Elawdan Ibrahim K , Alfkey Mohammed S. , Mousa Mahmoud M. , Eldrieny Ezzat A. 
Published: March 2023

Abstract
Prognosis in certain types of cancers still poor like in glioblastoma, some types of leukemia & lung cancer. Royal Jelly (RJ) chemical investigations started in 1960s and Major Royal Jelly proteins (MRJPs)were first reported in 1992. Later on, in 2006 after bee genome publication, nine different types of MRJPs were confirmed. Apisimin a new peptide found in RJ was discovered in 2002, and in 2004 Jelleines peptides group were also reported in RJ.
While Apisimin and Jellein 4 of not known biological activities recorded, Jellein 1, 2 &3 peptides showed antibacterial effect, antifungal effects, mast cell degranulation activity and hemolysis. However, nobody used Jelleins, Apisimin derivatives or both in a trial to treat cancer. Here we show new significant cancer inhibitions for a combination of Apisimin derivatives and modified Jelleins (with a change of few amino acids to d-form). In vivo studies, using a combination of peptide J1d (modified Jellein 1)and Ap3d( peptide contains 8 amino acids from c-terminal of Apisimin) in one group and a peptide K2( synthetic peptide consists of Ap3d + J1d) in another group in U-87 nude mice, we got significant inhibitions in both groups.
In another studies, using K2 peptide in one group and K3 peptide( synthetic peptide consists of Ap4d+ J2d) in another group versus C1498 and LLC syngeneic models, we also got significant inhibitions. We got no effect when using any Jelleins as well as with any Apisimin derivatives.
Our results demonstrate the effectiveness of using Apisimin derivatives peptides and modified Jelleins simultaneously in cancers.
The risk factors and mortality in elderly patients with different glomerular filtration rate after hip fracture
Authors: Wang Yishu , Zheng Songbai , Fan Yong qian , Zhu Yuefeng , Xu Leyang , Ye Zhibin , Hong Wei 
Published: March 2023

Abstract
Background: The aim of our study was to explore short- and long-term mortality and risk factors affecting Chinese elderly patients with different glomerular filtration rate after hip fracture.
Methods: This study included 513 patients aged≥65 years old and first been diagnosed with hip fracture in the Department Orthopedics of Huadong hospital between January 1st, 2010 and December 31st, 2011. Patients’ medical records were collected from the electronic medical record system. Survival status after hip fracture were assessed through telephone interviews and causes of death were verified. The patients were divided into three groups according to the renal function. Kaplan-Meier survival analysis was used to compare survival time between different groups. Univariate and multivariate Cox proportional hazards model was constructed to analyze differences between patients and factors associated with mortality.
Results: According to the inclusion and exclusion criteria and excluded patients who were lost to follow-up, a total of 325 patients were available in this study. Of which 73.5% were female and 85.2% received surgical operation. One-year cumulative mortality were 9.30%, 19.81% and 22.53% for group 1, 2 and 3. Five-year cumulative mortality were21.87%, 55.86% and 78.87%, while the ten-year cumulative mortality were40.61%, 74.32% and 94.37% respectively. Our analysis found that age (1.076 (1.031-1.122)), cognitive function (2.279(1.101-4.718)) were associated with higher risk of one-year mortality. Age (1.062(1.035-1.090)), eGFR<60 mL/min/1.73m2(2.425(1.054-5.578)) were associated with higher risk of five-year mortality. Age(1.073(1.049-1.097)), eGFR<60 mL/min/1.73m2(2.120(1.110-4.048)) and mildly decreased cognitive function(1.949(1.163-3.265)) were associated with higher risk of ten-year mortality.
Conclusion: Renal function seem to have little effect on short term mortality of Chinese elderly patients after hip fracture, but was an important risk factor affecting the medium to long-term mortality. For patients with decreased eGFR, fully evaluating the status of patients and choosing appropriate surgical method as soon as possible after fracture can greatly reduce the risk of death and ensure better survival in the elderly.
Experimental study of opto-thermal parameters of glycerol and ethylene glycol at various temperatures using laser thermal lens spectroscopy
Authors: keykeleh Zahra Safi , Mohammadi-Manesh Ebrahim , Mohebbifar Mohammad Reza 
Published: March 2023

Abstract
One of the important applications of the photothermal effect is laser thermal lens spectroscopy. Using this technique, some opto-thermal parameters of materials such as thermal diffusivity, thermo-optic coefficient (TOC), thermal conductivity, etc. can be accurately measured. In this experimental study the thermal diffusivity of butanol and propanol were measured. The accuracy of our experimental setup was confirmed by comparing with the reference. Then, the experiments were repeated for glycerol and ethylene glycol and measured the thermal diffusivity and thermal conductivity of these samples. A reliable agreement between these results and reference values was observed. Finally, thermal conductivity of glycerol and ethylene glycol at various temperatures was studied. These results show that thermal conductivity increases (from 0.2805 to 0.2865 W/mK for glycerol and from 0.246 to 0.275 W/mK for ethylene glycol) with increasing temperature (from 25°C to 90°C). Also, TOC and \(\frac{dn}{dQ}\) of these samples were measured at the beam waist of 25 µm and laser energy of 100 mJ.
Transcriptional Induction of NF-kB-Inducing Kinase by E2F4/5 Facilitates Collective Invasion of Glioma Cells
Authors: Pflug Kathryn , Lee Dong , McFadden Kassandra , Herrera Linda , Sitcheran Raquel 
Published: March 2023

Abstract
The prognosis of high-grade gliomas, such as glioblastoma multiforme (GBM), is extremely poor due to the highly invasive nature of these aggressive cancers. Previous work has demonstrated that TNF-weak like factor (TWEAK) induction of the noncanonical NF-κB pathway increases the invasiveness of glioma cells in an NF-κB-inducing kinase (NIK)-dependent manner. While NIK activity is predominantly regulated at the posttranslational level, we show here that NIK (MAP3K14) is upregulated at the transcriptional level in invading cell populations, with the highest expression observed in the most invasive cells. Glioma cells with high induction of NIK gene expression demonstrate characteristics of collective invasion, facilitating invasion of neighboring cells. Furthermore, we demonstrate that the E2F transcription factors E2F4 and E2F5 directly regulate NIK transcription and are required to promote glioma cell invasion in response to TWEAK. Overall, our findings demonstrate that transcriptional induction of NIK facilitates collective cell migration and invasion, thereby promoting glioma pathogenesis.
WITHDRAWN: Zn-MOF hydrogel: Regulation of ROS-mediated inflammatory microenvironment for treatment of atopic dermatitis
Authors: Qiu Lirong , Ouyang Chengcheng , Zhang Wei , Liu Jia , Yu Luting , Chen Guoguang , Ren Lili 
Published: March 2023

Abstract
Atopic dermatitis (AD) is a chronic and recurrent inflammation disease associated with immune dysfunction. The high level of reactive oxygen species (ROS) causes high oxidative stress and further results in the deterioration of AD. Here, a Zn-MOF hydrogel is prepared to regulate ROS-mediated inflammatory microenvironment. The hydrogel with high ROS scavenging performance has a good protective effect on cells. The antibacterial agent Zn-MOF(ZIF-8) loaded into the hydrogel (Gel@ZIF-8) can reduce its toxic and side effects and show a lasting and effective antibacterial activity. In the AD-induced mouse model, Gel@ZIF-8 can significantly enhance the therapeutic effect, such as reduce the thickness of epidermis, the number of mast cells and IgE antibodies. The results indicate that the ROS-scavenging hydrogel could treat the AD by regulating the inflammatory microenvironment, providing a promising treatment for managing AD.
The biocontrol agents of fall armyworm, Spodoptera frugiperda in Togo: moderating insecticide applications for natural control of the pest?
Authors: Koffi Djima , Agboka Komi , Adjevi Mawuko Kossi Anani , Adom Médétissi , Tounou Agbeko Kodjo , Meagher Robert L. 
Published: March 2023

Abstract
Although there has been intensive use of insecticides for fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) management, their effects on population reduction and performance of natural enemies have not been adequately investigated. Therefore, this study assessed the diversity and performance of natural biocontrol agents under insecticide and insecticide-free applications. Bio-agents were annually collected from 2016 to 2022 in 348 maize farms throughout the West African nation of Togo. The collections included the entomopathogenic nematode Ovomermis sinensis (Nematoda: Mermithidae), unidentified bacteria from Enterobacteriaceae and Enterococcus, unidentified viruses from Ascoviruses and Baculoviruses, and fungi Isaria spp. (Hypocreales: Clavicipitaceae) and Metarhizium rileyi (Hypocreales: Cordycipitaceae). Eggs were parasitized by Telenomus remus (Hymenoptera: Platygastridae), and the egg-larval parasitoid Chelonus bifoveolatus (Hymenoptera: Braconidae). Larval parasitoids included Anatrichus erinaceus (Diptera: Chloropidae), Archytas spp. and Lespesiaspp. (Diptera: Tachinidae), Bracon sp., Coccygidium luteum, Cotesia icipe and Meteoridea testacea (Hymenoptera: Braconidae), and Campoletis grioti and Ophion spp. (Hymenoptera: Ichneumonidae). The collected predators included Orius insidiosus (Heteropterans: Anthocoridae), Haematochares obscuripennis, Peprius nodulipes, Rhynocoris sp. and Zelus renardii (Heteroptera: Reduviidae), Calleida sp. (Coleoptera: Carabidae), Cheilomenes sulphurea, Coccinella septempunctata and Cycloneda sanguinea (Coleoptera: Coccinellidae), Euborellia annulipes, Forficula auricularia and F. senegalensis (Dermaptera: Forficulidae), Pheidole megacephala and Polyrhachis lamellidens (Hymenoptera: Formicidae), Chrysoperla carnea (Neuroptera: Chrysopidae), and Mantis religiosa(Mantodea: Mantidae). The parasitism rates were from 14.72% in 2018 to 45.38% in 2022 for egg masses, and from 1.32% in 2016 to 41.85% in 2021 for larvae. The parasitism rates were three to four times higher in unsprayed farms than sprayed farms.
Light exposure impairs hematological characteristics and oxidative stress markers in male African Giant Pouched Rat (Cricetomys gambianus) reared in captivity
Authors: Lavoisier Fonou Tadiesse , Julius Awah- Ndukum , Landri Tene Fotsing , Bertin Vémo Narcisse , Martine Tsambou Astride , Séverin Fonkem , Brice Menkem , Boris Takam Mbogne , Augustave Kenfack 
Published: March 2023

Abstract
African Giant Pouched Rat (AGPR) is widely used both to conduct research projects and for meat consumption. In order to improve its breeding and enhance its availability and accessibility, effects of light exposure on hematological and oxidative stress parameters have been studied. Twenty adult males AGPR weighing 945 ± 171g were randomly allocated into four photoperiod regimes either: 0L/24D, 12L/12D, 18L/06D, 24L/0D. Housed singly during 56 days, animals received food and water ad libitum . At the end of the trial, blood and serum were collected for studied parameters analysis. Results revealed a significant (p&lt;0.05) increase of the number of red blood cell (RBC), white blood cell (WBC), hemoglobin (HGB) and hematocrit (HCT) in animals kept in the darkness condition compared to those reared under extended photoperiods. Except for malondialdehyde (MDA) level which was not affected by photoperiod, catalase (CAT) and superoxide dismutase (SOD) activity increased significantly (p&lt;0.05) for any light exposure up to 12 hours per day. In conclusion, photoperiod affects the most hematological characteristics measured and has stressful effects in AGPR. This new knowledge provides a relevant information for the rearing environment of African Giant Pouched Rat.
Cortical thickness of the inferior parietal lobule as a potential predictor of relapse in men with alcohol dependence
Authors: Yang Kebing , Du Ruonan , Yang Qingyan , Zhao Rongjiang , Fan Fengmei , Chen Song , Luo Xingguang , Tan Shuping , Wang Zhiren , Yu Ting , Tian Baopeng , Le Thang M. , Li Chiang-Shan R. , Tan Yunlong 
Published: March 2023

Abstract
Background
Alcohol dependence (AD) is a disorder with a high recurrence rate that leads to a considerable public health burden. The risk of relapse appears to be related to a complex interplay of multiple factors. Herein, we aimed to explore the potential neural predictors of relapse in Chinese male patients with AD.
Methods
This study enrolled 58 male patients with AD who had undergone acute detoxification. General demographic information and clinical features were collected. Magnetic resonance imaging (MRI) data were used to measure cortical thickness across 34 regions of the brain. Patients were followed up at 6 months, and 51 patients completed the follow-up visit. These patients were divided into a relapser and an abstainer group. A binary logistic regression analysis was performed to investigate the potential risk factors of relapse.
Results
Compared to abstainers, relapsers showed higher inattention and non-planning impulsivity on the 11th version of the Barratt Impulsive Scale. The cortical thicknesses of the inferior-parietal lobule were significantly greater in abstainers compared with those in relapsers. Furthermore, binary logistic regression analysis showed that the thickness of the inferior parietal lobule predicted relapse.
Conclusions
Relapsers show poorer impulse control than abstainers, and MRI imaging shows a decreased thickness of the inferior parietal lobule in relapsers. Our results indicate the thickness of the inferior parietal lobule as a potential relapse predictor in male patients with AD.
A Multi-center study on Individualized Contrast Media Protocols in Abdominal Computed Tomography： Dose calculation based on the Liver Volume
Authors: Song Wei , Lyu Liang , Su Yu , Li Huaxiu , Chen Shouming , Li Junfeng , Wang Gang 
Published: March 2023

Abstract
Objectives: To evaluate the attenuation of a liver volume–adapted contrast protocol compared with the injection protocol based on body surface area in computed tomography of the abdomen.
Materials and Methods:1604 patients referred for unenhanced and multiphase enhanced abdominal CT scanning in a portal venous phase were included inthe study. 748 patients in Group 1 received injected iodine dose with the equation of liver volume (mL) × 7.225 + 16486.754 (mg). 856 patients in Group 2 received a body surface area-adapted protocol with the equation: 17600 mg iodine per m2. Attenuation (HU) in 8 segments of the liver was observed. Subjective image quality was assessed by the Likert scale. Differences between groups were statistically analyzed (P < 0.05 was considered statistically significant).
Results:No significant differences in baseline characteristics were found.The injected iodine differed significantly between groups (P < 0.001). The milligrams of iodine per kilogram in Group 1 was 445.59 ± 100.18 which was lower than previous studies of total body weight-adapted protocols with a better effect on higher weight population. Besides, a significant difference wasfoundinthe attenuation of the liver between the two groups.Group 1 showed better aggregation to the standard of 50 HU.
Conclusions: Liver volume-adapted contrast media protocol resulted in more homogeneous enhancement of the liver parenchyma and its enhancement efficiency is closer to the theoretical standard, whereas injected iodine can be reduced in a large percentage of the population.
Development and validation of a two glycolysis-related LncRNAs prognostic Signature for Glioma and in vitro analyses
Authors: Xu Xiaoping , Zhou Shijun , Tao Yuchuan , Zhong Zhenglan , Shao Yongxiang , Yi Yong 
Published: March 2023

Abstract
Background
Mounting evidence suggests that there is a complex regulatory relationship between long non-coding RNAs (lncRNAs) and the glycolytic process during glioma development. This study aimed to investigate the prognostic role of glycolysis-related lncRNAs in glioma and their impact on the tumor microenvironment.
Methods
This study utilized glioma transcriptome data from public databases to construct, evaluate, and validate a prognostic signature based on differentially expressed (DE)-glycolysis-associated lncRNAs through consensus clustering, DE-lncRNA analysis, Cox regression analysis, and receiver operating characteristic (ROC) curves. The clusterProfiler package was applied to reveal the potential functions of the risk score-related differentially expressed genes (DEGs). Finally, ESTIMATE and Gene Set Enrichment Analysis (GSEA) were utilized to evaluate the relationship between prognostic signature and the immune landscape of gliomas. Furthermore, the sensitivity of patients to immune checkpoint inhibitor (ICI) treatment based on the prognostic feature was predicted with the assistance of the Tumor Immune Dysfunction and Exclusion (TIDE) algorithm. Finally, qRT-PCR was used to verify the difference in the expression of the lncRNAs in glioma cells and normal cell.
Results
By consensus clustering based on glycolytic gene expression profiles, glioma patients were divided into two clusters with significantly different overall survival (OS), from which 2 DE-lncRNAs, AL390755.1 and FLJ16779, were obtained. Subsequently, Cox regression analysis demonstrated that all of these lncRNAs were associated with OS in glioma patients and constructed a prognostic signature with a robust prognostic predictive efficacy. Functional enrichment analysis revealed that DEGs associated with risk scores were involved in immune responses, neurons, neurotransmitters, synapses and other terms. Immune landscape analysis suggested an extreme enrichment of immune cells in the high-risk group. Moreover, patients in the low-risk group were likely to benefit more from ICI treatment. qRT-PCR results showed that the expression of AL390755.1 and FLJ16779 was significantly different in glioma and normal cells.
Conclusion
We constructed a novel prognostic signature for glioma patients based on glycolysis-related lncRNAs. Besides, this project had provided a theoretical basis for the exploration of new ICI therapeutic targets for glioma patients.
Assessment of Potential Health Risks Associated with Heavy Metal Contamination in Drinking Water in the Kulim Hi Tech Park (KHTP) Region of Malaysia
Authors: Zanon Muhamad Radzi , Ishak Ahmad Razali , Waras Maisarah Nasution , Abdullah Muhamad Azwat , Tualeka Abdul Rohim , Mohamed Rafeezul , Edinur Hisham Atan , Aziz Mohd Yusmaidie 
Published: March 2023

Abstract
The presence of heavy metals in the environment can have a negative impact on living beings and the environment. This study aimed to evaluate the potential human health risks associated with exposure to heavy metals in drinking water from Kulim Hi-Tech Park (KHTP), Malaysia. Thirty water samples were collected from different locations within and around KHTP, and the concentration of five heavy metals (iron (Fe), manganese (Mn), zinc (Zn), cadmium (Cd), and nickel (Ni)) was determined using atomic absorption spectroscopy. The mean concentration of these heavy metals ranged from 0.0177 ±0.0017 mg/L to 0.8652 ±0.0606 mg/L, with the mean concentration order being Ni> Fe> Zn > Mn> Cd. Iron, cadmium, and nickel exceeded the permissible limits. The Hazard Quotient (HQ) values for Fe, Zn, and Mn were ranked in the order of Fe > Zn > Mn. However, the individual and total HQ and Hazard Index (HI) values were below 1, suggesting no expected negative impact on human health. Nevertheless, the Excess Lifetime Cancer Risk (ELCR) values for Cd and Ni in the entire population of adults and children ranged from 2.35E-02 to 5.79E-03, indicating that the levels of Cd and Ni in water resources in KHTP and its surrounding area may be above safe levels and require proper remediation to minimize the risk to human health. The study establishes a baseline for heavy metal contamination in KHTP and nearby water resources, emphasizing the need for further research to safeguard Kulim's environment and residents.