bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of nomogram for predicting lymph node metastasis in early gastric cancer
Authors: He Jingyang , Cao Mengxuan , Li Enze , Hu Can , Zhang Yanqiang , Yu Pengcheng , Zhang Ruolan , Cheng Xiangdong , Xu Zhiyuan 
Published: August 2023

Abstract
Purpose:
To establish and verify a prediction model for lymph node metastasis (LNM) in early gastric cancer (EGC) and provide a reference for the selection of appropriate treatment for EGC patients.
Methods:
The clinicopathological data of 1584 patients with EGC admitted to Zhejiang Cancer Hospital from January 2010 to April 2019 were retrospectively analysed. Univariate and multivariate logistic regression analyses were used to explore the correlation between various clinicopathological factors and LNM in patients with EGC. Univariate K‒M and multivariate Cox regression analyses were used to explore the influence of multiple clinical factors on the prognosis of patients with EGC. The discrimination and calibration of the established prediction model, which is presented in the form of a nomogram, were also evaluated.
Results:
The incidence of LNM was 19.6%. Multivariate logistic regression analysis showed that tumour size, location, differentiation degree and pathological type were independent risk factors for LNM in EGC. Tumour pathological type and LNM were independent factors affecting the prognosis of patients with EGC. The area under the curve in the training and verification group was 0.750 (95% CI: 0.701 ~ 0.789) and 0.763 (95% CI: 0.687 ~ 0.838), respectively. The calibration curve showed good agreement between the predicted and actual probability, and decision curve analysis indicated strong clinical practicability.
Conclusion:
Tumour diameter ≥ 2 cm, poor differentiation degree, middle-lower tumour location and signet ring cell carcinoma were identified as independent risk factors for LNM in EGC. Among them, tumour pathological type and LNM were independent risk factors for prognosis in EGC. This clinical model for predicting LNM in EGC was used to construct a nomogram, which has high diagnostic value and can serve as a reference in clinical treatment selection.
PLANTdex: An index to assess the multi-dimensional environmental impact of global crop commodities
Authors: jwaideh Mark , Dalin Carole 
Published: August 2023

Abstract
This study presents the croP environmentaL sustAiNabiliTy index (PLANTdex), one of the first spatially explicit (5 arcmin resolution) indexes to measure environmental impacts caused by the production of sixteen crop commodities globally. PLANTdex comprises five globally standardized environmental impact intensity indicators developed from robust spatially-explicit environmental models related to climate change, water stress and terrestrial and aquatic biodiversity loss. Due to variations in PLANTdex, the index was able to differentiate production system efficiencies and environmental sensitivities, locating environmentally sustainable and unsustainable production globally. Due to PLANTdex's build, the index identified a more significant number of synergies (e.g., between biodiversity indicators) existed than trade-offs (e.g., between biodiversity-related GHG emissions and marine biodiversity loss) between indicators. The analysis identified over 32 regions that could collaborate on agricultural management strategies based on production, physical characteristics and environmental impact sensitivities that allow for the exchange of knowledge on sustainable agriculture. PLANTdex has the potential to support initiatives such as the Taskforce on Nature-related Financial Disclosures (TNFD) and E.S.G. strategies at the due diligence stage.
Analgesic effect of intraoperative intravenous S(+)-ketamine during total knee arthroplasty (TKA) surgery: Study protocol for a randomized controlled clinical trial Analgesic effect of intraoperative intravenous S(+)-ketamine during total knee arthroplasty (TKA) surgery
Authors: Deng Shi-Yuan , Chen Li-Na , Song Xiao , Guo Liang , Zhao Fei , Liu Jing , Dong Ling 
Published: August 2023

Abstract
Introduction:
Poor management of acute pain after total knee arthroplasty (TKA) increases the risk of complications in elderly patients. S(+)-ketamine in subanaesthetic doses is available as an adjuvant to the standard regimen of opioids, presenting prominent analgesic effects and opioid-sparing effects. Appropriate multimodal medication projects are required to decrease postoperative opioid use and improve effects in elderly patients undergoing TKA.
Methods
This is a randomized, placebo-controlled trial. A total of 144 participants aged ≥ 65 years old undergoing TKA will be randomly allocated into the S(+)-ketamine and placebo groups in a 1:1 ratio. S(+)-ketamine or placebo will be intravenously administered at 0.3 mg/kg/h during the operation by anesthesiologists. Blinded evaluation by trained investigators will be completed at 2 hours, 24 hours and 48 hours after surgery. The primary outcome measure is the numeric rating scale (NRS) score at rest and movement at 24 hours after surgery. The secondary outcomes include the NRS scores at rest and movement at 2 hours and 48 hours after surgery, the number of patients who require additional analgesics during the first 48 hours after operation, the total consumption of opioids or nonsteroid anti-inflammatory drugs during the first 48 hours after operation and adverse events at 2,24, 48 hours after operation.
Trial registration number:
NCT05289050
Examining Changes in Pain Interference via Pandemic-Induced Isolation Among Patients Receiving Medication for Opioid Use Disorder: A Secondary Data Analysis
Authors: Frohe Tessa , Janssen Tim , Garner Bryan R. , Becker Sara J. 
Published: August 2023

Abstract

• Background
Early in the pandemic, the United States population experienced a sharp rise in the prevalence rates of opioid use, social isolation, and pain interference. Given the high rates of pain reported by patients on medication for opioid use disorder (MOUD), the pandemic presented a unique opportunity to disentangle the relationship between opioid use, pain, and social isolation in this high-risk population. We tested the hypothesis that pandemic-induced isolation would partially mediate change in pain interference levels experienced by patients on MOUD, even when controlling for baseline opioid use. Such work can inform the development of targeted interventions for a vulnerable, underserved population.
• Methods
Analyses used data from a cluster randomized trial (N = 188) of patients on MOUD across eight opioid treatment programs. As part of the parent trial, participants provided pre-pandemic data on pain interference, opioid use, and socio-demographic variables. Research staff re-contacted participants between May and June 2020 and 133 participants (71% response rate) consented to complete a supplemental survey that assessed pandemic-induced isolation. Participants then completed a follow-up interview during the pandemic that again assessed pain interference and opioid use. A path model assessed whether pre-pandemic pain interference had an indirect effect on pain interference during the pandemic via pandemic-induced isolation.
• Results
Consistent with hypotheses, we found evidence that pandemic-induced isolation partially mediated change in pain interference levels among MOUD patients during the pandemic. Higher levels of pre-pandemic pain interference and opioid use were both significantly associated with higher levels of pandemic-induced isolation. In addition, pre-pandemic pain interference was significantly related to levels of pain interference during the pandemic, and these pain levels were partially explained by the level of pandemic-induced isolation reported.
• Conclusions
Patients on MOUD with higher use of opioids and higher rates of pain pre-pandemic were more likely to report feeling isolated during COVID-related social distancing and this, in turn, partially explained changes in levels of pain interference. These results highlight social isolation as a key risk factor for patients on MOUD and suggest that interventions promoting social connection could be associated with reduced pain interference, which in turn could improve patient quality of life.
Trial registration:
NCT03931174
Can seaweed extracts from different seasons exhibit differential plant biostimulant properties?
Authors: Santos Janaína Pires , Torres Priscila , Amorim Ana Maria , Silva Beatriz Nogueira Torrano da , Santos Deborah Yara Alves Cursino dos , Chow Fungyi 
Published: August 2023

Abstract
A wide variety of commercial seaweed-based biostimulants is available worldwide for improving plant growth and development for agriculture and gardening purposes. Biostimulant properties are influenced by species, seasonality, preparation methods, and harvest site. This study evaluated the biostimulant properties of the methanolic, hot aqueous and aqueous extracts of three Brazilian macroalgae - Sargassum vulgare C.Agardh (Ochrophyta, Phaeophyceae), Palisada flagellifera (J.Agardh) K.W.Nam (Rhodophyta), and Ulva fasciata Delile (Chlorophyta) - in two different periods (dry and wet seasons) on germination and early development of Lactuca sativa Linnaeus. All three algae increase the total length of lettuce seedlings, with the root length being the primary factor that exhibited growth enhancement. The length of the roots increased by an average of 40% when in contact with the extracts, ranging from 28–55% for P. flagellifera, 37–48% for S. vulgare, and 28–79% for U. fasciata. The extracts promoted resource allocation for elongation of roots, which can aid the plant survival by improving competition for water and nutrients. In general, the biostimulant properties of seaweed extracts varied between harvesting period, since the activity of methanolic and aqueous extracts were influenced by the seasons. Methanolic extracts from the dry season and aqueous extracts from the wet season showed the most biostimulant effect, although overall, the dry season extracts were the most effective. In conclusion, the studied extracts of three Brazilian macroalgae have significant biostimulant properties, which can vary depending on the harvesting period and extract type.
Development and psychometric properties of Clinical Learning Environment Scale for Chinese nursing students
Authors: XU Yun , ZHENG Yi , Hui-Mei WANG , Fang HUANG 
Published: August 2023

Abstract
Objective
To develop a clinical learning environment scale for Chinese nursing students and test its reliability and validity.
Methods
Based on Moos social environment theory, qualitative interviews and expert consultations were used to develop the pre-test version of the Chinese Nursing Students' Clinical Learning Environment Scale. With a convenience sampling method, 255 and 1582 Chinese nursing students were selected as the prediction and validation samples from December 2022 to March 2023. Exploratory and confirmatory factor analyses were conducted to determine the final version of the scale and test its reliability and validity.
Results
The scale consisted of 19 items. Exploratory factor analysis extracted three characteristic roots in terms of goal oriented, interpersonal relation, and learning support, which explained 71.500% of the total variance. The results of the confirmatory factor analysis showed that the GFI was 0.848, AGFI was 0.806, RMSEA was 0.090, RMR was 0.041, NFI was 0.910, IFI was 0.916, CFI was 0.916, PCFI was 0.798, and PNFI was 0.793. The reliability values of the three dimensions were 0.870, 0.858, and 0.943, respectively, and the convergent validity values were 0.574, 0.603, and 0.625, respectively.
Conclusion
The reliability and validity of the dimensions of the Chinese Nursing Students' Clinical Learning Environment Scale are acceptable, which can be used as a useful tool to measure the clinical learning environment of Chinese nursing students.
Inflammatory bowel disease may increase the risk of COPD: a two-sample Mendelian randomization study
Authors: Chen Shiyu , Li Xiaojian , Xie Rongfang , Qiu Guirong , Liu Zhongyong , Lan Zhihui 
Published: August 2023

Abstract
Background: The potential relationship between IBD and COPD remains uncertain. necessitating further exploration of their causal relationship. To address this, we employed Mendelian randomization in the present study to investigate the potential causal link between IBD and COPD and provide valuable insights into their interconnectedness.
Methods: We performed a comprehensive two-sample Mendelian randomization analysis utilizing extensive genetic summary data obtained from genome-wide association studies (GWAS).Our study utilized a comprehensive dataset comprising a substantial cohort, including 12,366 ulcerative colitis (UC) cases and 33,609 controls, 25,042 IBD cases and 34,915 controls, and 12,194 Crohn's disease (CD) cases and 28,072 controls. The COPD dataset consisted of 58,925 individuals from various GWAS studies. Our main analyses utilized the IVW method with a random-effects model, complemented by WME and MR-Egger approaches.
Results: Our study demonstrated a significant correlation between genetic predisposition to IBDand the risk of developing COPD, as evidenced by the IVW(odds ratio [OR] = 1.02, 95% CI = 1.00-1.04, p = 0.013). Consistent results were obtained using the WME (OR = 1.02, 95% CI = 1.00-1.04, p = 0.032) and MR-Egger (OR = 1.02, 95% CI = 1.00-1.04, p = 0.032) methods. Importantly, our analysis did not reveal any evidence of directional pleiotropy between IBD and rosacea, as confirmed by both funnel plots and MR-Egger intercepts. Subgroup analysis further indicated a robust association between Crohn's disease (CD) and rosacea (IVW: OR = 1.01, 95% CI = 1.00-1.02, p = 0.008), while the causal association between ulcerative colitis (UC) and rosacea did not reach statistical significance (IVW: OR = 1.01, 95% CI = 0.99-1.02, p = 0.169). These findings provide compelling evidence supporting the link between IBD and COPD, shedding light on potential pathogenic mechanisms underlying these conditions.
Conclusions: Our comprehensive MR analysis provides robust evidence of a unidirectional positive association between IBD and COPD, with varying degrees of association observed among different subtypes of IBD. Notably, variations in the SMAD3 gene may play a pivotal role in the increased risk of COPD among individuals with IBD. Recognizing the elevated occurrence of COPD in IBD patients holds significant clinical implications, highlighting the importance of early identification, monitoring, patient education, preventive measures, and collaborative treatment approaches involving healthcare professionals. The TGF-β1/SMAD3 pathway could serve as a promising therapeutic target for managing IBD complicated with COPD.
Cultural adaptation and validation of the Sinhala version of the Frail Non- Disabled Tool (FiND)
Authors: Silva Shehan , Ramadasa Udayangani , Lekamwasam Sarath 
Published: August 2023

Abstract
Background
Frailty, a common geriatric syndrome of vulnerability, is associated with a decline in health and function. The most problematic expression of population ageing is associated with weakness, slowing, decreased energy, lower activity and when severe, unintended weight loss. Frailty is not consciously identified in clinical practice and is not widely studied in Sri Lanka. A validated tool for screening frailty in a busy clinical setting is therefore much needed. This study was done as a part of validating the Sinhala version of the Frail Non-Disabled (S-FiND) tool.
Methods
The FiND tool was translated from English to Sinhala by two translators, blinded to each other. They were combined and translated back to the original language by two separate translators. After verifying the content validity, unambiguity and clarity of items in a focused group discussion, the pre-final version was piloted among 30 volunteers. After assessing the psychometric properties of the pre-final version, the final version was tested among 100 adults older than 65 years from the Colombo South Teaching Hospital. The tool was compared with Fried’s frailty phenotype taken as the gold standard.
Results
Data were analysed for the agreement with the reference standard, the Fried Phenotype. The mean (SD) age of subjects was 73.9 (7.8) years. The overall time taken to fill out the questionnaire was 2 min. The agreement (Kappa) between the S-FiND questionnaire and the Fried phenotype was 0.7 (P < 001). The sensitivity and specificity of FiND in detecting frailty were 92% and 74%, respectively. The agreements (Kappa) between the individual items of S-FiND; involuntary loss of weight/ more than 4.5 kg over one year, the feeling of effort/ not getting going and level of physical activity, with the Fried phenotype, were 0.28 (p = 0.001), 0.06 (p = 0.045) and 0.339 (p < 0.001). respectively. When subjects were categorized frail and robust based on FiND, frail subjects reported a higher incidence of falls (50%) during the previous 12 months, compared to those robust (13%) (p < 0.001 for Chi stat)
Conclusion
The S-FiND is a reliable, valid and well-received tool that can be used in detecting the frailty of non-disabled Sinhala-speaking older adults.
PDPN+ CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX
Authors: Xia Juan , Li Yaoyin , Ma Zeyi , Li Weiyu , Xu Xiaoqing , Shen Peiqi , Zhang Sien , Cheng Bin 
Published: August 2023

Abstract
Cancer-associated fibroblasts (CAFs) are abundant and heterogeneous in tumor microenvironment. Cross-talk between cancer cells and CAFs results in cancer progression. Here, we demonstrated that a distinct cancer-associated fibroblasts with podoplanin (PDPN) positive expression (PDPN+ CAFs) was correlated with poor survival in oral squamous cell carcinoma (OSCC). PDPN+ CAFs promoted the progression of OSCC by transferring exosomal lncRNA FTX to OSCC cells. Mechanistically, FTX bound to flap endonuclease-1 (FEN1), forming an RNA‒protein complex. FTX enhanced promoter demethylation of FEN1 by recruiting ten-eleven translocation-2 (TET2). In addition, FTX/FEN1 axis promoted OSCC cells motility by inhibiting ferroptosis. In xenograft experiments, RSL-3, a ferroptosis-inducing agent, suppressed the tumorigenesis potential of FEN1-overexpressed OSCC cells. Furthermore, ACSL4 was confirmed to participate in the motility promotion induced by FEN1 overexpression. FEN1 could bound to promoter region of Acyl-CoA synthetase long-chain family member 4 (ACSL4) and then inhibit ferroptosis in OSCC cells. Our study reveals that PDPN+ CAFs promote the invasiveness of OSCC cells by inhibiting ferroptosis through FTX/FEN1/ACSL4 signaling cascade. PDPN+ CAFs may serve as a novel potential therapeutic target for OSCC.
Leveraging circulating microbiome signatures to predict tumor immune microenvironment and prognosis of patients with non-small cell lung cancer
Authors: Zhou Xiaohan , You Liting , Xin Zhaodan , Su Huiting , Zhou Juan , Ma Ying 
Published: August 2023

Abstract
Background
Accumulating evidence supports the significant role of human microbiome in development and therapeutic response of tumors. Circulating microbial DNA is non-invasive and could show a general view of the microbiome of host, making it a promising biomarker for cancers. However, whether circulating microbiome is associated with prognosis of non-small cell lung cancer (NSCLC) and its potential mechanisms on tumor immune microenvironment still remains unknown.
Methods
The blood microbiome data and matching tumor RNA-seq data of TCGA NSCLC patients were obtained from Poore’s study and UCSC Xena. Univariate and multivariate Cox regression analysis were used to identify circulating microbiome signatures associated with overall survival (OS) and construct the circulating microbial abundance prognostic scoring (MAPS) model. Nomograms integrating clinical characteristics and circulating MAPS scores were established to predict OS rate of NSCLC patients. Integrated analysis of multi-omics was used to deciphered the tumor microenvironment landscape of patients in circulating MAPS-high and MAPS-low groups. Finally, the predictive value of circulating MAPS on the efficacy of immunotherapy and chemotherapy were assessed.
Results
A circulating MAPS prediction model consisting of 14 circulating microbes was constructed and had an independent prognostic value for NSCLC. The integration of circulating MAPS into nomograms may improve the prognosis predictive power. Multi-omics analysis revealed potential interactions between prognostic circulating microbiome and tumor immune microenvironment. Especially, intratumor plasma cells and humoral immune response were enriched in circulating MAPS-low group, while intratumor CD4 + Th2 cells and proliferative related pathways were enriched in MAPS-high group. Finally, drug sensitivity analysis indicated the potential of circulating MAPS as a predictor of chemotherapy efficacy.
Conclusion
A circulating MAPS prediction model was constructed successfully and showed great prognostic value for NSCLC. Our study provides new insights of interactions between microbes, tumors and immunity, and may further contribute to precision medicine for NSCLC.
Larvicidal potential and molecular docking analysis of metabolites from new Egyptian bacterial strains as effective biological control agents against Culex pipiens (L: Diptera)
Authors: Mansour Tokaa , Radwan Wafaa H. , Mansour Menna , Gomaa Mohamed , Shepl Mohamed , Farouk Farouk , Soliman Ahmed G. , ElHalim Basma T. Abd- , El-Senosy Mohamed M.K. , bakry Ashraf , Ebeed Naglaa , Alsenosy Neima K. , Elhariry Hesham , galal Ahmed , El-Sayed Salwa M. , Adly Eslam , Abu-Hussien Samah H. 
Published: August 2023

Abstract
Mosquito control in Egypt depends on applying chemical synthetic pesticides that impact negatively on human health and the environment as well as the development of antibiotic and chemical resistance. This study aims to control the 3rd and 4th instars of Culex pipines larvae using four bacterial strains. According to Phenotypic and molecular identification, the four isolates were identified as Bacillus subtilis MICUL D2023, Serratia marcescens MICUL A2023, Streptomyces albus LARVICID, and Pseudomonas fluorescens MICUL B2023. All strains were deposited in GenBank under accession numbers OQ764791, OQ729954, OQ726575, and OQ891356, respectively. Larvicidal activity of all microbial strain metabolites against a field strain of C. pipines explored low LC50 results and reached its lowest values on the 3rd day with values of 6.40, 38.4, and 46.33 for P. fluorescens, S. albus, and S. marcescens, respectively. In addition, metabolites of P. fluorescence were more toxic than those of S. albus, followed by S. marcescens. B. subtilis shows no larvicidal effect on both field and lab mosquito strains. Microscopic alterations of 3rd and 4th instars showed toxic effects on different body parts (thorax, midgut, and anal gills), including losing external hairs, abdominal breakage, and larvae shrinkage, as well as different histological malformations in the digestive tract, midgut, and cortex. GC-MS analysis detected 51, 30, and 32 different active compounds from S. albus, S. marcescens, and P. fluorescens, respectively. GC detected 1, 2-BENZEA2:A52NEDICARBOXYLIC ACID, 2-Cyclohexene-1-carboxylic-acid-5-2-butenyl-methyl ester, and 3 octadecahydro2R3S4Z9Z-11R-12S from S. albus, S. marcesens, and P. fluorescens, respectively. Total protein, Total carbohydrate, and Acetylcholine esterase activity indicated significantly low levels on the 3rd day. All strain metabolites were safe against HSF cell lines. The docking results confirmed the role of the produced metabolites as larvicidal agents and Acetylcholine esterase inhibition. Such a problem need more studies on applying more and more natural pesticides.