bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sense and Consent in Cocreating with Earth Others
Authors: Morehouse Harlan , Morse Cheryl 
Published: March 2023

AbstractRecent debates around multispecies communities emphasize collaboration across difference for fostering intimate relations with the world. The basic premise is simple: a richer understanding of the ways in which we are connected to the world will yield greater care for the world. However, while collaboration across difference might close conceptual and material gaps between self and other, and nature and society, it is not always clear whether or how collaboration should take place. Indeed, largely absent in these debates are matters concerning cross-species consent. It can be challenging to obtain consent or ascertain agreement in the absence of straightforward communication. To address the whether and how of collaboration across difference, this article draws on ethnographic research on dowsing—a traditional method for finding underground water and other invisible or intangible resources—in the United States and the United Kingdom. This research shows how dowsers establish dialogue by attuning to Earth Others (e.g., water, plants, spirits) using various tools, such as dowsing rods, pendulums, and their own bodies. This article addresses how practitioners apply dowsing as a technique for communicating across human and more-than-human divides through ethical inquiries that tend to the agency and seek the consent of Earth Others in matters concerning land use. This research suggests that dowsing offers a reciprocal and dialogic strategy for collaborating with that which is often unseen, unheard, or ignored.
Patient Frailty Influences Outcome After Open, But Not Minimally Invasive, Transforaminal Interbody Fusion: A Case Series of 115 Patients With Lumbar Degenerative Disease
Authors: Burke John F. , Garcia Joseph H. , Safaee Michael M. , Wong Christopher , Gill Sabraj A. , Chou Dean , Mummaneni Praveen V. , Deviren Vedat , Ames Christopher P. , Clark Aaron J. 
Published: March 2023

BACKGROUND:
For transforaminal lumbar interbody fusion (TLIF), there are equally good open and minimally invasive surgery (MIS) options.

OBJECTIVE:
To determine if frailty has a differential effect on outcome for open vs MIS TLIF.

METHODS:
We performed a retrospective review of 115 TLIF surgeries (1-3 levels) for lumbar degenerative disease performed at a single center; 44 MIS transforaminal interbody fusions and 71 open TLIFs were included. All patients had at least a 2-year follow up, and any revision surgery during that time was recorded. The Adult Spinal Deformity Frailty Index (ASD-FI) was used to separate patients into nonfrail (ASD-FI < 0.3) and frail (ASD-FI > 0.3) cohorts. The primary outcome variables were revision surgery and discharge disposition. Univariate analyses were performed to reveal associations in demographic, radiographic, and surgical data with the outcome variables. Multivariate logistic regression was used to assess independent predictors of outcome.

RESULTS:
Frailty uniquely predicted both reoperation (odds ratio 8.1, 95% CI 2.5-26.1, P = .0005) and discharge to a location other than home (odds ratio 3.9, 95% CI 1.2-12.7, P = .0239). Post hoc analysis indicated that frail patients undergoing open TLIF had a higher revision surgery rate (51.72%) compared with frail patients undergoing MIS-TLIF (16.7%). Nonfrail patients undergoing open and MIS TLIF had a revision surgery rate of 7.5% and 7.7%, respectively.

CONCLUSION:
Frailty was associated with increased revision rate and increased probability to discharge to a location other than home after open transforaminal interbody fusions, but not MIS transforaminal interbody fusions. These data suggest that patients with high frailty scores may benefit from MIS-TLIF procedures.
MafB-dependent neurotransmitter signaling promotes β cell migration in the developing pancreas
Authors: Bsharat Sara , Monni Emanuela , Singh Tania , Johansson Jenny K. , Achanta Kavya , Bertonnier-Brouty Ludivine , Schmidt-Christensen Anja , Holmberg Dan , Kokaia Zaal , Prasad Rashmi B. , Artner Isabella 
Published: March 2023

ABSTRACT
Hormone secretion from pancreatic islets is essential for glucose homeostasis, and loss or dysfunction of islet cells is a hallmark of type 2 diabetes. Maf transcription factors are crucial for establishing and maintaining adult endocrine cell function. However, during pancreas development, MafB is not only expressed in insulin- and glucagon-producing cells, but also in Neurog3+ endocrine progenitor cells, suggesting additional functions in cell differentiation and islet formation. Here, we report that MafB deficiency impairs β cell clustering and islet formation, but also coincides with loss of neurotransmitter and axon guidance receptor gene expression. Moreover, the observed loss of nicotinic receptor gene expression in human and mouse β cells implied that signaling through these receptors contributes to islet cell migration/formation. Inhibition of nicotinic receptor activity resulted in reduced β cell migration towards autonomic nerves and impaired β cell clustering. These findings highlight a novel function of MafB in controlling neuronal-directed signaling events required for islet formation.
Actin-capping protein regulates actomyosin contractility to maintain germline architecture in C. elegans
Authors: Ray Shinjini , Agarwal Priti , Nitzan Anat , Nédélec François , Zaidel-Bar Ronen 
Published: March 2023

ABSTRACT
Actin dynamics play an important role in tissue morphogenesis, yet the control of actin filament growth takes place at the molecular level. A challenge in the field is to link the molecular function of actin regulators with their physiological function. Here, we report an in vivo role of the actin-capping protein CAP-1 in the Caenorhabditis elegans germline. We show that CAP-1 is associated with actomyosin structures in the cortex and rachis, and its depletion or overexpression led to severe structural defects in the syncytial germline and oocytes. A 60% reduction in the level of CAP-1 caused a twofold increase in F-actin and non-muscle myosin II activity, and laser incision experiments revealed an increase in rachis contractility. Cytosim simulations pointed to increased myosin as the main driver of increased contractility following loss of actin-capping protein. Double depletion of CAP-1 and myosin or Rho kinase demonstrated that the rachis architecture defects associated with CAP-1 depletion require contractility of the rachis actomyosin corset. Thus, we uncovered a physiological role for actin-capping protein in regulating actomyosin contractility to maintain reproductive tissue architecture.
Zasp52 strengthens whole embryo tissue integrity through supracellular actomyosin networks
Authors: Ashour Dina J. , Durney Clinton H. , Planelles-Herrero Vicente J. , Stevens Tim J. , Feng James J. , Röper Katja 
Published: March 2023

ABSTRACT
During morphogenesis, large-scale changes of tissue primordia are coordinated across an embryo. In Drosophila, several tissue primordia and embryonic regions are bordered or encircled by supracellular actomyosin cables, junctional actomyosin enrichments networked between many neighbouring cells. We show that the single Drosophila Alp/Enigma-family protein Zasp52, which is most prominently found in Z-discs of muscles, is a component of many supracellular actomyosin structures during embryogenesis, including the ventral midline and the boundary of the salivary gland placode. We reveal that Zasp52 contains within its central coiled-coil region a type of actin-binding motif usually found in CapZbeta proteins, and this domain displays actin-binding activity. Using endogenously-tagged lines, we identify that Zasp52 interacts with junctional components, including APC2, Polychaetoid and Sidekick, and actomyosin regulators. Analysis of zasp52 mutant embryos reveals that the severity of the embryonic defects observed scales inversely with the amount of functional protein left. Large tissue deformations occur where actomyosin cables are found during embryogenesis, and in vivo and in silico analyses suggest a model whereby supracellular Zasp52-containing cables aid to insulate morphogenetic changes from one another.
Pax4-Ghrelin mediates the conversion of pancreatic ε-cells to β-cells after extreme β-cell loss in zebrafish
Authors: Yu Junqin , Ma Jianlong , Li Yanfeng , Zhou Yang , Luo Lingfei , Yang Yun 
Published: March 2023

ABSTRACT
Pancreatic ε-cells producing ghrelin are one type of endocrine cell found in islets, which have been shown to influence other intra-islet cells, especially in regulating the function of β cells. However, the role of such cells during β-cell regeneration is currently unknown. Here, using a zebrafish nitroreductase (NTR)-mediated β-cell ablation model, we reveal that ghrelin-positive ε-cells in the pancreas act as contributors to neogenic β-cells after extreme β-cell loss. Further studies show that the overexpression of ghrelin or the expansion of ε-cells potentiates β-cell regeneration. Lineage tracing confirms that a proportion of embryonic ε-cells can transdifferentiate to β-cells, and that the deletion of Pax4 enhances this transdifferentiation of ε-cells to β-cells. Mechanistically, Pax4 binds to the ghrelin regulatory region and represses its transcription. Thus, deletion of Pax4 derepresses ghrelin expression and causes producing more ghrelin-positive cells, enhancing the transdifferentiation of ε-cells to β-cells and consequently potentiating β-cell regeneration. Our findings reveal a previously unreported role for ε-cells during zebrafish β-cell regeneration, indicating that Pax4 regulates ghrelin transcription and mediates the conversion of embryonic ε-cells to β-cells after extreme β-cell loss.
Regulation of morphogen pathways by a Drosophila chondroitin sulfate proteoglycan Windpipe
Authors: Koh Woo Seuk , Knudsen Collin , Izumikawa Tomomi , Nakato Eriko , Grandt Kristin , Kinoshita-Toyoda Akiko , Toyoda Hidenao , Nakato Hiroshi 
Published: March 2023

ABSTRACT
Morphogens provide quantitative and robust signaling systems to achieve stereotypic patterning and morphogenesis. Heparan sulfate (HS) proteoglycans (HSPGs) are key components of such regulatory feedback networks. In Drosophila, HSPGs serve as co-receptors for a number of morphogens, including Hedgehog (Hh), Wingless (Wg), Decapentaplegic (Dpp) and Unpaired (Upd, or Upd1). Recently, Windpipe (Wdp), a chondroitin sulfate (CS) proteoglycan (CSPG), was found to negatively regulate Upd and Hh signaling. However, the roles of Wdp, and CSPGs in general, in morphogen signaling networks are poorly understood. We found that Wdp is a major CSPG with 4-O-sulfated CS in Drosophila. Overexpression of wdp modulates Dpp and Wg signaling, showing that it is a general regulator of HS-dependent pathways. Although wdp mutant phenotypes are mild in the presence of morphogen signaling buffering systems, this mutant in the absence of Sulf1 or Dally, molecular hubs of the feedback networks, produces high levels of synthetic lethality and various severe morphological phenotypes. Our study indicates a close functional relationship between HS and CS, and identifies the CSPG Wdp as a novel component in morphogen feedback pathways.
A ballistic pollen dispersal strategy based on stylar oscillation ofHypochaeris radicata(Asteraceae)
Authors: Ito Shuto , Rajabi Hamed , Gorb Stanislav N. 
Published: March 2023

ABSTRACTAsteraceae, one of the largest flowering plant families, are adapted to a vast range of ecological niches. Their adaptability is partially based on their strong ability to reproduce. The initial, yet challenging, step for the reproduction of animal-pollinated plants is to transport pollen to flower-visiting pollinators. We adopted Hypochaeris radicata as a model species to investigate the functional morphology of the typical floral feature of Asteraceae, a pollen-bearing style. Using quantitative experiments and numerical simulations, here we show that the pollen-bearing style can serve as a ballistic lever for catapulting pollen grains to pollinators. This can potentially be a pollen dispersal strategy to propel pollen to safe sites on pollinators' bodies, which are beyond the physical reach of the styles. Our results suggest that the specific morphology of the floret and the pollen adhesion avoid pollen waste by catapulting pollen within a specific range equal to the size of a flowerhead. The insights into the functional floral oscillation may shed light on the superficially unremarkable, but ubiquitous functional floral design of Asteraceae.
Influence of the Solvent on the Assembly of Ni3(hexaiminotriphenylene)2 Metal–Organic Framework Nanosheets at the Air/Liquid Interface
Authors: Ohata Takashi , Tachimoto Kazuaki , Takeno Kanokwan Jumtee , Nomoto Akihiro , Watanabe Takeshi , Hirosawa Ichiro , Makiura Rie 
Published: March 2023

Abstract
Nanosheets of metal–organic frameworks (MOFs)—porous crystalline materials consisting of metal ions and organic ligands—are actively studied for their intrinsic chemical/physical properties attributed to the reduced dimensionality and for their potential to function as ideal components of nanodevices, especially when electrical conduction is present. Air/liquid interfacial synthesis is a promising technique to obtain highly oriented MOF nanosheets. However, rational control of size and shape combined with the aimed functionality remains an important issue to address making it necessary to research the critical factors governing nanosheet characteristics in the interfacial synthesis. Here, we investigate the influence of the solvent—methanol (MeOH) versus N,N-dimethylformamide (DMF)—used to prepare a ligand spread solution on an assembly of MOF nanosheets composed of Ni2+ and 2,3,6,7,10,11-hexaiminotriphenylene (HITP) (HITP-Ni-NS). We find that the macroscopic morphological uniformity in the micrometer scale is higher when DMF is used as the solvent. Regarding the microscopic crystalline domain, molecules of DMF with relatively high polarity and boiling point are involved in HITP-Ni-NS formation, hindering its growth and resulting in nanosheets with slightly smaller lateral size than that grown when MeOH is used. These findings provide crucial guidelines towards establishing a judicious strategy for creating desired MOF nanosheets at the air/liquid interface, thereby driving forward research on both fundamental and applied aspects of this field.
Privacy and Market Concentration: Intended and Unintended Consequences of the GDPR
Authors: Johnson Garrett A. , Shriver Scott K. , Goldberg Samuel G. 
Published: March 2023

We show that websites’ vendor use falls after the European Union’s (EU’s) General Data Protection Regulation (GDPR), but that market concentration also increases among technology vendors that provide support services to websites. We collect panel data on the web technology vendors selected by more than 27,000 top websites internationally. The week after the GDPR’s enforcement, website use of web technology vendors falls by 15% for EU residents. Websites are relatively more likely to retain top vendors, which increases the concentration of the vendor market by 17%. Increased concentration predominantly arises among vendors that use personal data, such as cookies, and from the increased relative shares of Facebook and Google-owned vendors, but not from website consent requests. Although the aggregate changes in vendor use and vendor concentration dissipate by the end of 2018, we find that the GDPR impact persists in the advertising vendor category most scrutinized by regulators. Our findings shed light on potential explanations for the sudden drop and subsequent rebound in vendor usage. This paper was accepted by Matthew Shum, marketing. Funding: Financial support from the Marketing Science Institute and the George Mason University Program on Economics & Privacy is gratefully acknowledged. Supplemental Material: The web appendix and data are available at https://doi.org/10.1287/mnsc.2023.4709 .
Management of Pediatric Community-Acquired Pneumonia in the Era of Widespread Vaccination against Streptococcus Pneumoniae at a First-Level Hospital
Authors: Ragusa Neftj , Gianotto Nefer Roberta , Barbara Affif , Luotti Diego , Peano Luca , Bogliatto Fabrizio , Berger Massimo 
Published: March 2023

Community-acquired pneumonia (CAP) is a common and potentially severe infection affecting children. A retrospective study was carried out at our Institution between November 2022 and January 2023. Twenty-eight patients under 14 years of age were diagnosed and treated accordingly. Median age was 35 months, half of the patients were males. The most detected pathogen was S. pneumoniae (in 36% of all patients). Other pathogens detected were RSV in 3 patients (11%), influenza B virus in two patients (7%), adenovirus in two patients (7%), and M. pneumoniae in one patient (4%). Fourteen children (50% of the total number) developed a respiratory failure that required supplemental oxygen. Among these, 8 children (57% of all children receiving oxygen supplementation) required low-flow oxygen delivery, four children (29%) required high-flow oxygen delivery, and one child (7%) required invasive ventilation. Five children (55% of children with a bacterial CAP) received intravenous antibiotics, while 44% of patients received oral antibiotics. First-line intravenous antibiotics consisted of a third-generation cephalosporin for infants and children older than 1 month, and ampicillin/sulbactam with gentamicin for newborns. Amoxy-clavulanic acid was the first-line oral antibiotic for pneumococcal CAP, while oral clarithromycin was the first line antimicrobic for CAP caused by M. pneumoniae.
Twenty-one children (75% of total patients) were fully vaccinated, three children (11%) had received two doses because of age. Regarding the 10 children with a pneumococcal CAP, seven (70%) were fully vaccinated, one had received two-doses of pneumococcal conjugate vaccine (PCV), and two were unvaccinated.
Decision impact studies, evidence of clinical utility for genomic assays in cancer: A scoping review
Authors: Parker Gillian , Hunter Sarah , Ghazi Samer , Hayeems Robin Z. , Rousseau Francois , Miller Fiona A. 
Published: March 2023

BackgroundDecision impact studies have become increasingly prevalent in cancer prognostic research in recent years. These studies aim to evaluate the impact of a genomic test on decision-making and appear to be a new form of evidence of clinical utility. The objectives of this review were to identify and characterize decision impact studies in genomic medicine in cancer care and categorize the types of clinical utility outcomes reported.MethodsWe conducted a search of four databases, Medline, Embase, Scopus and Web of Science, from inception to June 2022. Empirical studies that reported a “decision impact” assessment of a genomic assay on treatment decisions or recommendations for cancer patients were included. We followed scoping review methodology and adapted the Fryback and Thornbury Model to collect and analyze data on clinical utility. The database searches identified 1803 unique articles for title/abstract screening; 269 articles moved to full-text review.Results87 studies met inclusion criteria. All studies were published in the last 12 years with the majority for breast cancer (72%); followed by other cancers (28%) (lung, prostate, colon). Studies reported on the impact of 19 different proprietary (18) and generic (1) assays. Across all four levels of clinical utility, outcomes were reported for 22 discrete measures, including the impact on provider/team decision-making (100%), provider confidence (31%); change in treatment received (46%); patient psychological impacts (17%); and costing or savings impacts (21%). Based on the data synthesis, we created a comprehensive table of outcomes reported for clinical utility.ConclusionsThis scoping review is a first step in understanding the evolution and uses of decision impact studies and their influence on the integration of emerging genomic technologies in cancer care. The results imply that DIS are positioned to provide evidence of clinical utility and impact clinical practice and reimbursement decision-making in cancer care.Systematic review registration:Open Science Frameworkosf.io/hm3jr.
The impact of influenza vaccination on surgical outcomes in COVID-19 positive patients: An analysis of 43,580 patients
Authors: Taghioff Susan M. , Slavin Benjamin R. , Mehra Shefali , Holton Tripp , Singh Devinder 
Published: March 2023

Background
Multiple recent studies suggest a possible protective effect of the influenza vaccine against severe acute respiratory coronavirus 2 (SARS-CoV-2). This effect has yet to be evaluated in surgical patients. This study utilizes a continuously updated federated electronic medical record (EMR) network (TriNetX, Cambridge, MA) to analyze the influence of the influenza vaccine against post-operative complications in SARS-CoV-2-positive patients.

Methods
The de-identified records of 73,341,020 patients globally were retrospectively screened. Two balanced cohorts totaling 43,580 surgical patients were assessed from January 2020-January 2021. Cohort One received the influenza vaccine six months-two weeks prior to SARS-CoV-2-positive diagnosis, while Cohort Two did not. Post-operative complications within 30, 60, 90, and 120 days of undergoing surgery were analyzed using common procedural terminology(CPT) codes. Outcomes were propensity score matched for characteristics including age, race, gender, diabetes, obesity, and smoking.

Results
SARS-CoV-2-positive patients receiving the influenza vaccine experienced significantly decreased risks of sepsis, deep vein thrombosis, dehiscence, acute myocardial infarction, surgical site infections, and death across multiple time points(p<0.05, Bonferroni Correction p = 0.0011). Number needed to vaccinate (NNV) was calculated for all significant and nominally significant findings.

Conclusion
Our analysis examines the potential protective effect of influenza vaccination in SARS-CoV-2-positive surgical patients. Limitations include this study’s retrospective nature and reliance on accuracy of medical coding. Future prospective studies are warranted to confirm our findings.
Psychological interventions for generalized anxiety disorder: Effects and predictors in a naturalistic outpatient setting
Authors: Krzikalla Clara , Morina Nexhmedin , Andor Tanja , Nohr Laura , Buhlmann Ulrike 
Published: March 2023

Objective
Numerous randomized controlled trials (RCTs) demonstrate the efficacy of cognitive behavioral therapy (CBT), metacognitive therapy (MCT), and methods to reduce intolerance of uncertainty (IU-CBT) in the treatment of generalized anxiety disorder (GAD). However, few studies have investigated these treatments under conditions of routine clinical care. The main objective of this study was to investigate the effectiveness of psychotherapy for GAD in an outpatient setting and to identify factors influencing treatment outcome.

Methods
Fifty-nine GAD patients received naturalistic CBT (including MCT and IU-CBT) in an outpatient clinic and postgraduate training center for psychotherapy. Patients completed self-report questionnaires at the beginning and end of therapy regarding the main outcome worry as well as metacognitions, intolerance of uncertainty, depression, and general psychopathology.

Results
Worry, negative metacognitions, intolerance of uncertainty, depression, and general psychopathology decreased significantly (p’s < .001) with large effect sizes for all symptoms (d = 0.83–1.49). A reliable change in the main outcome worry was observed in 80% of patients, and recovery occurred in 23%. Higher worry scores at posttreatment were predicted by higher pretreatment scores, female sex, and less change in negative metacognitive beliefs during treatment.

Conclusions
Naturalistic CBT for GAD appears to be effective in routine clinical care for worry as well as depressive symptoms, with particular benefits associated with altering negative metacognitions. However, a recovery rate of only 23% is lower than the rates reported in RCTs. Treatment needs to be improved, especially for patients with more severe GAD and for women.