bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of probiotic, prebiotic or synbiotic supplementation after Roux-en-Y gastric bypass: a systematic review with meta-analysis
Authors: Cabanillas-Lazo Miguel , Pascual-Guevara Milagros , Hilario-Gomez Maryori M. , Salazar-Alarcón Jorge L. , León-Figueroa Darwin A. , Caballero-Alvarado Jose A. , Barboza Joshuan J. 
Published: June 2023

Abstract
The Roux-en-Y gastric bypass (RGYB) is a type of bariatric surgery that, despite its benefits, is associated with multiple gastrointestinal and nutritional complications, so using the microbiota as a therapeutic target can enhance its benefits and reduce the risk of complications. Therefore, this study aimed to evaluate the effects of probiotic, prebiotic or synbiotic supplements in patients with morbid obesity undergoing bariatric Roux-en-Y surgery. A systematic literature review was conducted using PubMed, Scopus, Web of Science, Ovid-Medline, Cochrane library and Embase databases through January 2022. The search strategy identified 766 articles, of which, after selection process, four randomized clinical trial (RCTs) studies were included. Twelve percent of patients were men, and the mean age ranged between 32.0 and 48.6 years. Three trials included probiotic supplementation, one included two types of interventions (prebiotic and symbiotic supplementation). Three were conducted in Brazil, one in United States. Regarding the percentage of excess weight loss (%EWL), with very low certainty, in two RCTs that including 144 participants, probiotic supplementation did not reduce significantly %EWL compared to placebo ([MD]: -2.38; 95% CI: -6.11, 1.35; I2 = 0%). Probiotic supplementation reduced discomfort measured by Gastric Symptom Rating Scale ([MD]: -0.50; 95% CI: -0.63, -0.37), reduce triglycerides blood levels ([MD]: -0.21.8 mg/dl; 95% CI: -24.1, -19.5) and increase B12 vitamin ([MD]: 18.2 pmol/L; 95% CI: 14.2, 22.2) compared to placebo.
Impact of Junction Temperature on High-Frequency Performance of Double avalanche Region IMPATT Oscillator
Authors: Mukhopadhyay Sangeeta Jana , Ghivela Girish Chandra 
Published: June 2023

Abstract
This study investigates the impact of junction temperature on the high-frequency performance of a DAR (Double Avalanche Region) IMPATT oscillator, operating at several frequency bands. By using a simulation technique created by the authors, the temperature dependency of static (DC) and high frequency properties are observed. The large-signal admittance features reveal the existence of distinct, widely spaced negative conductance bands, which can result in a broad tuning capability. The linear and non-linear changes of high frequency parameters have been examined as a function of temperature. These will be extremely helpful for the optimum thermal design of ATT (Avalanche transit time) devices.
Skin mucosal immune-parameters and expression of the immune-relevant genes in Danio rerio treated by white button mushroom (Agaricus bisporus)
Authors: Ezatrahimi Narmin , Soltanian Siyavash , Hoseinifar Seyed Hossein 
Published: June 2023

Abstract

This study evaluates using different levels of the white button mushroom powder (WBMP) on some mucosal innate immune parameters (lysozyme, protease, esterase, alkaline phosphatase activities, and total immunoglobulin levels), and the relative expression of some principal immune-relevant genes (lysozyme, TNF-α, and IL-1β) in the zebra danio intestine. Zebrafish specimens (1.75 ± 0.25 g) were divided into experimental units based on the additives to a diet including 5, 10, and 20 g of WBMP per kg of food weight, alone or in conjunction with the antibiotic (10 mg/kg BW), and the AGRIMOS (1 g/kg food weight). Following the 11-day experimental duration, the skin mucus and intestine were sampled. To assess the immune gene expression, the real-time PCR detection system was conducted according to the ΔΔCt method using the IQ5 software (Bio-RAD). Results showed that all groups had a significant increase in terms of mucosal lysozyme activity compared to the control group. Examination of total immunoglobulin, protease, esterase, and ALP activity in fish under experimental treatment showed that there was no significant difference between the trial groups and the control groups. The most expression of the lysozyme gene was related to the group that was separately taken the lower concentration (5 g per kg of FW) of WBMP. In conclusion, the amount of 1% mushroom powder in the diet can improve its immune function. Our recommendation is that given the positive effects that mushroom powder added on the diet alone, avoid taking antibiotics for this purpose.
Our Moon experiences COVID-19 global lockdown effect !
Authors: PRASAD K DURGA , AMBILY G 
Published: June 2023

Abstract
Systematic investigation of lunar night-time temperatures can possibly be thought as a stable platform to study Earth’s radiation budget and climate change as advocated earlier by several researchers. In this study, we report an interesting observation possibly of changing Earth’s climate as experienced by the Moon, utilising a rare and novel context of COVID-19 global lockdown. Lunar night-time surface temperatures of six different sites on the Moon’s nearside were analysed during the period 2017-2020. Results showed an anomalous dip in the lunar night time surface temperatures for all the sites during April-May 2020, the COVID-19 global lockdown period, when compared to the values of the same period in the past. Since the terrestrial radiation has also showed a significant reduction during that time, the anomalous decrease observed in lunar surface temperatures is attributed to the COVID 19 global lockdown. Therefore, the study shows that the Moon has possibly experienced the effect of COVID 19 lockdown visualised as an anomalous decrease in lunar night-time surface temperatures during that period. These results can be substantiated further from Moon-based observatories in future, thereby making them potential tools for observing Earth’s environmental and climate changes.
Influences of occupational exposure on cognitive functions, job stress, and burnout levels: A cross-sectional study among Iranian firefighters
Authors: mehrifar Younes , Nikooyan Faezeh , Samimi Kazem , Pirami Hamideh 
Published: June 2023

Abstract
Background
Firefighters are exposed to job stress and burnout in the workplace, which can impair their cognitive functions.
Methods
This descriptive-correlational study was conducted in three Iranian fire stations. Sixty-one participants were studied, including 34 firefighters in the exposure group and 27 office workers in the control group. Job stress and burnout levels were measured using standardized job stress and burnout questionnaires, and cognitive functions were assessed using two computerized tests Stroop and continuous performance.
Results
The mean age and work experience of firefighters were 33.29 ± 9.44 and 10.42 ± 3.51 years, respectively. The firefighters’ job stress and burnout levels were, on average, 163.12 ± 9.18 and 88.89 ± 4.28, respectively, which were significantly higher than those of the control group (P < 0.05). The results of the Pearson correlation test revealed a strong correlation (r = 0.719) between job stress and burnout. Pearson correlation coefficient (r= -0.512-0.757), yielded from psychological tests, demonstrated that firefighters’ job stress and burnout had moderate to strong associations with certain indicators of Stroop and continuous performance tests.
Conclusion
This study demonstrates that firefighters experience high levels of job stress and burnout. In light of the significant associations between certain indicators of Stroop and continuous performance tests and job stress and burnout levels, it is possible to conclude that job stress and burnout reduce the cognitive functions of firefighters. However, further research is recommended in this field.
Integrating Transcriptome and Metabolome to Explore the Growth-Promoting Mechanisms of GABA in Blueberry Plantlets
Authors: Liu Mingfeng , Bai Mingyue , Yue Jiajia , Fei Xiaoke , Xia Xiuying 
Published: June 2023

Abstract
Background
Tissue culture technology is the main method for the commercial propagation of blueberry plants, but blueberry plantlets grow slowly and have long growth cycles under in vitro propagation, resulting in low propagation efficiency. In addition, the long culturing time can also result in reduced nutrient content in the culture medium, and the accumulation of toxic and harmful substances that can lead to weak growth for the plantlets or browning and vitrification, which ultimately can seriously reduce the quality of the plantlets. Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that can improve plant resistance to various stresses and promote plant growth, but the effects of its application and mechanism in tissue culture are still unclear.
Results
In this study, the effects of GABA on the growth of in vitro blueberry plantlets were analyzed following the treatment of the plantlets with GABA. In addition, the GABA-treated plantlets were also subjected to a comparative transcriptomic and metabolomic analysis. The exogenous application of GABA significantly promoted growth and improved the quality of the blueberry plantlets. In total, 2,626 differentially expressed genes (DEGs) and 377 differentially accumulated metabolites (DAMs) were detected by comparison of the control and GABA-treated plantlets. Most of the DEGs and DAMs were involved in carbohydrate metabolism and biosynthesis of secondary metabolites.
Conclusions
The comprehensive analysis results indicated that GABA may promote the growth of blueberry plantlets by promoting carbon metabolism and nitrogen assimilation, as well as increasing the accumulation of secondary metabolites such as flavonoids, steroids and terpenes.
Clinical implementation PI-RADS v2.1: Detection rate of clinically significant prostate cancer in PI-RADS 4 and 5 categories–The Real-life data
Authors: Tirtashi Reza Habibi , Tamaskani Narges , Salamat Fatemeh , Ghazimoghaddam Behrouz , Niakan Babak , Nematollahi Nematollah , Gharib Mohammad Hadi 
Published: June 2023

Abstract
Background
Clinically significant prostate cancer (csPCa) diagnosis and risk assessment have been improved after utilizing multiparametric magnetic resonance imaging (mp-MRI). To access the global standardization in mp-MRI acquisition and interpretation, Prostate Imaging Reporting and Data System version 2.1 (PI-RADS v2.1) was developed and showed good overall performance in the detection of csPCa, particularly among higher categories. This study aimed to identify the csPCa detection rate (DR) among PI-RADS 4 and 5 categories.
Methods
The study population includes 76 (109 lesions) biopsy naïve patients with PI-RADS scores of 4 and 5 in prebiopsy mp-MRI who underwent systematic biopsy combined with cognitive targeted biopsy (cog-MRGB) between March 2020 and September 2022. Baseline characteristics and pathological findings were compared based on csPCa and PI-RADS scores. In addition, prostate cancer (PCa) and csPCa DRs were determined overall and according to the PI-RADS categories and the prostate zones.
Results
Among the study population, 35 men (46.10%) (51 lesions) and 41 men (53.90%) (58 lesions) belonged to PI-RADS scores of 4 and 5, respectively. The age of participants was 68.74 years in mean (with a standard deviation [SD] 8.83). Moreover, the level of prostate-specific antigen (PSA) was 9.47 ng/ml in the median (interquartile range [IQR] 6.20-19.79), along with the median prostate volume of 45.50 (IQR 35.00–76.00) ml. Individuals suffering from csPCa had statistically significant higher levels of PSA, PI-RADS categories, and lesions located on the prostate peripheral zone (PZ), as well as larger lesions and smaller prostate. The overall PCa and csPCa DRs were 57.90% (44 patients) and 44.70% (34 patients). Furthermore, csPCa DR stratified by PI-RADS scores and prostate zones was 68.30% in PI-RADS 5, 17.10% in PI-RADS 4, 54.90% in the PZ, and 34.30% in the prostate transition zone (TZ).
Conclusions
Our real-life data from a Middle Eastern developing country showed that the clinical implementation of PI-RADS v2.1 in combination with cog-MRGB yielded an acceptable overall PCa and csPCa DR, particularly among PI-RADS scores of 5. However, the results provided notable limitations on the PI-RADS score of 4 and TZ.
Simulated Nursing Grand Rounds in The Clinical Education of Postgraduate Nursing Students
Authors: Khoshbakht-Pishkhani Maryam , Javadi-Pashaki Nazila , Noveiry Marzieh Jahani- Sayad , Asgari Fariba 
Published: June 2023

Abstract
Introduction: As a novel teaching technique, educational simulations can expand students’ knowledge, skills, and performance. This study was carried out to design, implement, and evaluate simulated nursing grand rounds in promoting the learning of clinical nursing care in postgraduate nursing students at Guilan University of Medical Sciences.
Materials and methods: In this quasi-experimental study, all postgraduate nursing students of Shahid Beheshti School of Nursing and Midwifery, Rasht, in the second semester of the academic year 2021-2022, were invited to take their clinical credits. Each training credit was held in six days by attending nursing grand rounds in the clinical setting with the relevant professor and peers and participating in the simulated grand rounds at the skill lab. The data collection tool consisted of a two-section questionnaire, with the first part containing a demographic profile and a questionnaire about satisfaction with the simulated grand round (STROEHLEIN & SPENCER,2020)
Results: Twelve second-semester postgraduate nursing students participated in this study. The mean age of the participants in this study was 25.58 ± 2.67, and the field of most of them was internal surgery (67.7%). The wards where the training course was held included cardiac 58.3% and ICU 41.7%. Evaluating students’ opinions about this teaching method indicated that 80-100% were satisfied with the simulated nursing grand round. The majority of students (83.3%) believed that the method was very effective and beneficial in analyzing complex patients.
Conclusion: The simulated grand round intensifies students’ awareness and satisfaction and helps the professor apply the theoretical concepts educated in the classroom in the clinical setting. Therefore, this method can be a valuable tool to expand nursing students’ learning in clinical settings.
The evaluation of the electrochemical properties of Co3O4 nanopowders synthesized by autocombustion and sol-gel methods
Authors: Kalpana S , Bhat Vinay S. , Hegde G. , Prabhu T. Niranjana , Anantharamaiah P.N. 
Published: June 2023

Abstract
In present work, we investigated the synthesis methods for producing spherical-shaped nanomaterials of spinel cobalt oxide (Co3O4) as electrode materials. Two synthesis methods, autocombustion (Co3O4-AC) and sol-gel (Co3O4-SG), were employed to synthesize cobalt oxide nanopowders with a spherical shape. Characterization techniques including XRD, TEM, BET, and XPS analyses were conducted to evaluate the synthesized samples. The phase purity of the cobalt oxide samples were probed using XRD analysis and the crystallite size was determined to be 44 nm for Co3O4-AC and 36 nm for Co3O4-SG. TEM analysis further confirmed the desired spherical morphology of the particles. The surface area of Co3O4-AC was found to be 15 m2/g, while Co3O4-SG exhibited a slightly lower surface area of 11 m2/g. The energy storage experiments were conducted in terms of CV and GCD to enhance the electrochemical performance of the samples. Co3O4-AC exhibited a higher Cs 162 F/g at Im (current density) 0.25 A/g, indicating its superior energy storage capability. On the other hand, Co3O4-SG shows a Cs 98 F/g, indicating slightly lower execution compared to Co3O4-AC. Both nanomaterials exhibited excellent stability, showing no degradation over 1000 charge-discharge cycles. Overall, the study successfully synthesized spherical-shaped cobalt oxide nanomaterials using autocombustion and sol-gel methods. The obtained results demonstrate the promising energy storage properties of Co3O4-AC and Co3O4-SG, with Co3O4-AC exhibiting higher specific capacitance.
Overview of Major Cutaneus and Subcutaneus Skin Problems in Horses and Their Associated Risk Factor in and Around Bishoftutown, Ethiopia
Authors: Nagara Alemayo 
Published: June 2023

Abstract
The most significant animals in Ethiopia's packaging and transportation systems are horses. Despite the fact that illness and other issues significantly hinder their performance, their socioeconomic contribution is typically underestimated, jeopardizing their welfare. As a result, this cross-sectional study was conducted to determine the main skin problem concerning working horses in and around the town of Bishoftu, as well as the associated risk factors. For the study, 414 working horses in total were included in the study. A questionnaire survey and a clinical examination of a number of horses were carried out at the same time. It was revealed that 60% of horses have skin problems. The study's skin problems were wound (41.3%), epizootic lymphangiti (16.4%), and sarcoids (1.9%), in decreasing order. The occurrence of wounds varied significantly (x2 = 15.66, p = 0.05) depending on the horse's body condition score; horses in poorer body condition were more vulnerable to wounds than horses in higher body condition. Age-related differences in wound up prevalence were statistically significant (p = 0.05), showing that older horses were more susceptible. More injuries occurred as a result of poor harness and saddle design (x2 = 409.93, P = 0.000). The chest region of the body had a slightly higher percentage of wounds (24%). Depending on the type of work, there was a significant difference in the prevalence of epizootic lymphangitis (x2 = 4.0028, p= 0.05), showing that cart horses were more severely affected than pack horses. However, there was no discernible variation according to housing system, age, or bodily condition score. Between horses that had received protection and those that had not, there was a significant variation in the prevalence of sarcoids; the non-housed horses had a proportionately higher prevalence than the sheltered horses. The anatomical distribution of the lesions on the horses suggested a correlation between areas of improper hobbling, packing, and harnessing. According to this study, wounds are the main source of skin issues for working horses in the area. To address the current issue, a comprehensive equine health programme should be put in place.
Deciphering the Molecular Mechanisms Underlying the Psoriasis and T2D: A Systems Biology Approach Using Microarray Data Analysis
Authors: Yang Li , Zhang Lei , Du Qingfang , Tian Jun 
Published: June 2023

Abstract
Background Although a large number of evidence has identified that psoriasis is significantly correlated with type 2 diabetes (T2D), the common molecular mechanism of its occurrence remains unclear. Our study aims to further elucidate the mechanism of the occurrence of this complication.Methods We obtained the gene expression data of psoriasis (GSE30999) and T2D (GSE28829) from the Gene Expression Omnibus (GEO) dataset. Then the common differentially expressed genes (DEGs) of T2D and psoriasis were identified. After that, we performed three types of analyses about these DEGs, including functional enrichment analysis, protein-protein interaction (PPI) network and module manufacture, hub genes identification and co-expression analysis.Results 132 common DEGs (14 upregulated genes and 118 downregulated genes) were identified for subsequent a series of analyses. Function enrichment analysis demonstrated that Rap1 signaling pathway, PI3K-Akt signaling pathway, and cGMP-PKG signaling pathway may play a significant role in pathogenesis of psoriasis and T2D. Finally, 3 important hub genes were selected by utilizing cytoHubba, including SNRPN, GNAS, IGF2.Conclusions Our work reveals the common pathogenesis of psoriasis and T2D. These Hub genes and common signaling pathways provide insights for further investigation of molecular mechanism about psoriasis and T2D.
The Incidence and Risk Factors Related to Post Operative Dysphagia After Anterior Cervical Spine Surgery: A Prospective Study
Authors: Singh Jagdeep , Singh Navpreet , Gupta Pranav , Bansal Kapil , CHANDHAR ANMOL 
Published: June 2023

Abstract
BACKGROUND
Post-surgical dysphagia is one complication particularly common in early postoperative period after Anterior cervical spine surgery (ACSS). However, the pathophysiology of dysphagia after has not been well understood. This study aimed to analyze the frequency and risk factors for developing swallowing difficulty following anterior cervical spine procedures and find an effective program to prevent and treat.
METHODS
A prospective observational study was conducted on 50 patients undergoing ACSS from April 2021 to Oct 2022 at the Department of Orthopedics, Guru Gobind Singh Medical College and Hospital, Punjab (India). Patients were in the age group of 27 to 60 years. The indications for cervical surgeries were traumatic, degenerative, infective and neoplastic involving C2 to C7 vertebra with signs of neural compression unresponsive to conservative treatment. Data on patient gender, age, BMI, duration of surgery, surgery blood loss, segment operated and the number of segments operated were collected. ( Table 1) Follow up time was 24 weeks.
RESULTS
Incidence of dysphagia was 20% (10/50) within first week which reduced to zero at completion of 6 months of follow-up. Dysphagia was present in 2% (1/50) patients in age group 21–40 years and 18% (9/50) patients in age group of 41–60 years. 14.6% (6/41) males and 44% (4/9) of females had dysphagia. Prevalence of patients involving one segment was 9.5% (4/42), two segmants was 80% (4/5) and three levels was 50% (1/2). Mean duration of surgery in patients with post operative dysphagia was 115 mins. Mean blood loss in patients with post-operative dysphagia was 171.40 mL Mean Et (endotracheal) tube cuff pressure in patients with post operative dysphagia was 24.70 cm H2O. Within the first week post surgery, there were 10 cases out of which one was mild, six were moderate and three were severe.
CONCLUSION
Despite the fact that some inconsistency is there in the literature regarding risk factors it can be safely concluded from our study that incidence of post operative dysphagia can be reduced by decreasing blood loss during surgery, reducing surgery time and optimizing Endotracheal tube cuff pressure during surgery.
Large-scale proteomics analysis of five brain regions from Parkinson’s disease patients with a GBA1 mutation
Authors: Blumenreich Shani , Nehushtan Tamar , Kupervaser Meital , Shalit Tali , Gabashvili Alexandra , Joseph Tammar , Milenkovic Ivan , Hardy John , Futerman Anthony 
Published: June 2023

Abstract

Despite being the second most common neurodegenerative disorder, little is known about Parkinson’s disease (PD) pathogenesis. A number of genetic factors predispose towards PD, among them mutations inGBA1, which encodes the lysosomal enzyme acid-β-glucosidase. We now perform non-targeted, mass spectrometry based quantitative proteomics on five brain regions from PD patients with aGBA1mutation (PD-GBA) and compare to age- and sex-matched idiopathic PD patients and controls. Two proteins were differentially-expressed in all five brain regions whereas significant differences were detected between the brain regions, with changes consistent with loss of dopaminergic signaling in the substantia nigra, and activation of a number of pathways in the cingulate gyrus, including ceramide synthesis. Mitochondrial oxidative phosphorylation was inactivated to a larger extent in IPD samples in most brain regions compared to controls and to a larger extent in PD-GBA. This is the first large-scale proteomics dataset generated for the study of PD-GBA.