bluuebunny/crossref_metadata_2025_split huggingface dataset:
Grain yield and adaptation of spring wheat to Norwegian growing conditions is driven by allele frequency changes at key adaptive loci discovered by genome-wide association mapping
Authors: Mróz Tomasz , Dieseth Jon Arne , Lillemo Morten 
Published: May 2023

Abstract
Grain yields in Norwegian spring wheat increased by 18 kg ha− 1 per year between 1972 and 2019 due to introduction of new varieties. These gains were associated with increments in the number of grains per spike and extended length of the vegetative period. However, little is known about the genetic background of this progress. To fill this gap, we conducted genome-wide association study on a panel consisting of both adapted (historical and current varieties and lines in the Nordics) and important not adapted accessions used as parents in the Norwegian wheat breeding program. The study concerned grain yield, plant height, and heading and maturity dates, and detected 12 associated loci, later validated using independent sets of recent breeding lines. Adaptation to the Norwegian cropping conditions is associated with the Vrn-A1 locus, and a previously undescribed locus on chromosome 1B associated with heading date. Two loci associated with grain yield, corresponding to the TaGS5-3A and Ta-Col5 loci, indicated historical selection pressure for high grain yield. A locus on chromosome 2A explained the tallness of the oldest accessions. We investigated the origins of the beneficial alleles associated with the wheat breeding progress in the Norwegian material, tracing them back to crosses with Swedish, German, or CIMMYT lines. This study contributes to the understanding of wheat adaptation to the Norwegian growing conditions, sheds light on the genetic basis of historical wheat improvement and aids future breeding efforts by discovering loci associated with important agronomic traits in wheat.
Thick-Wire Swing Arc Narrow Gap GMA Welding Assisted by Pre-embedding Cold Wires
Authors: Zhu Zhengyu , Wang Jiayou , Liu Shubin , Jiang Yuqing , Zhu Jie , Li Xuyang , Zhang Luxiao 
Published: May 2023

Abstract
To raise narrow gap GMA welding efficiency while ensuring weld formation, the present work presents a thick wire swing arc narrow gap GMA welding process assisted by cold wires. This process employs the thick wire and the pre-embedded cold wires to jointly increase the welding efficiency, while improving the groove sidewall penetration by the swing arc. Two types of thick wires, being conventional single-strand welding wire (SWW) and cable-type welding wire (CWW), are respectively used as main wire. After solving the series arc problem during CWW arc-igniting, the effects of the pre-embedded wire layer number on narrow gap weld formation and welding efficiency are investigated for the thick wire welding. Experimental results show that the weld thickness and the sidewall penetration do not obviously change with increasing the number of the cold wires, while the weld layer height significantly grows. Additionally, the SWW weld formation looks more sound. Compared to the thin-wire welding, the thick wire welding without the cold wire raises the deposition rate by about 38% and 58% as respectively using SWW and CWW. When pre-embedding two layers of cold wires, the increased percentage of the deposition rate further rises respectively to about 81% and 112%. These thus demonstrate the effectiveness of the developed process in addition to the higher efficiency of the CWW welding.
Infection with COVID-19 promotes the progression of pancreatic cancer through the PI3K-AKT signaling pathway
Authors: Zhang Xusheng , Chen Bendong , Liu Kejun , Ma Yongxin , Liu Yimin , Zhou Hongcai , Wei Peng 
Published: May 2023

Abstract
Objective To investigate the effect of COVID-19 infection on pancreatic cancer .
Methods Based on the mRNA-Seq data of COVID-19 patients and pancreatic cancer (PC) patients in the GEO database, we used support vector machine (SVM), LASSO-Cox regression analysis and random forest tree (RF) to screen the common signature genes of the two diseases and further investigate their effects and functional characteristics on PC, respectively. And the above procedures were performed in R software.
Results The proteins COL10A1/FAP/FN1 were found to be common signature genes for COVID-19 and PC, were significantly up-regulated in both diseases, and showed good diagnostic efficacy for PC. The risk model based on COL10A1/FAP/FN1 showed good PC risk prediction ability and clinical application potential. Tumor typing based on COL10A1/FAP/FN1 expression levels effectively classified PC into different subtypes, and showed significant differences between the two subtypes in terms of survival prognosis, immune levels, immune checkpoint expression levels, mutation status of common tumor mutation sites, and drug sensitivity analysis. While pathway analysis also revealed that FN1 as an extracellular matrix component may be involved in the biological process of PC by regulating the PI3K-AKT signaling axis.
Conclusion The upregulated expression of COL10A1/FAP/FN1, the characteristic genes of COVID-19, are potential diagnostic targets for PC, and the upregulated expression of FN1 may promotes the progression of PC by activating the PI3K-AKT signaling pathway. The COL10A1/FAP/FN1-based typing provides a new typing approach for PC, also provides a good reference and idea for the refinement of PC treatment and subsequent clinical research.
Modeling of above-ground biomass of pure Calabrian pine (Pinus brutia Ten.) stands using Sentinel-2 time series and Sentinel-1 imagery; a case study from the south of Turkey
Authors: AKSOY Hasan 
Published: May 2023

Abstract
Aboveground biomass (AGB) is an important source of information for natural resource conservation at spatial scales, accurate determination of carbon stocks and climate containment systems, and sustainable resource conservation planners. This study, it was aimed to model the Above-ground biomass (AGB) of pure Calabrian pine (Pinus brutia Ten.) stands in southern Turkey. In the study, digital band, reflectance, and vegetation indices values obtained from Sentinel-2 satellite images of rainy, semi-arid and dry seasons were used to measure the effect of seasonality on the modeling of the AGB. Digital band, reflectance (VV/VH polarization), and backscatter values were also used from the Sentinel-1 satellite image. In addition to all these, topographic data of the study area was used as an independent variable. The multiple linear regression (MLR) method was used in the modeling of AGB and the most successful model was tried to be determined by the relative rank method. A total of 33 models were obtained by creating data sets with the independent variables first one by one and then with each other in combination. The highest model success of AGB was obtained in the data set created by the integration of digital band and vegetation indices, Sentinel-1 (S1) reflectance, and topographic values of Sentinel-2 (S2) images taken in rainy and semi-arid seasons (R2=0.49; RMSE=26.21 ton ha-1; MAE=22.50 ton ha-1; BIC=5.42; AIC= 21.23; Rj=57.68). It was observed that the integration of topographic data into each dataset increased the modeling success in all models. In addition, it was observed that the highest success in modeling the AGB with the data obtained from Sentinel-2 was obtained in the rainy season data. In particular, it was seen that the combination of S2, S1, and topographic data would contribute to the prediction accuracy of AGB.
Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex
Authors: Sverchinsky Dmitry V. , Alhasan Bashar A. , Mikeladze Marina A. , Lazarev Vladimir F. , Kuznetzova Lubov’ S. , Morshneva Alisa V. , Nikotina Alina D. , Zivana Amr , Koludarova Lidia V. , Margulis Boris A. , Guzhova Irina V 
Published: May 2023

Abstract
Background. Cancer recurrence is regulated by a variety of factors, among which is the material of dying tumor cells; it is suggested that remaining after anti-cancer therapy tumor cells receive a signal from proteins called damage-associated molecular patterns (DAMPs), one of which is heat shock protein 70 (Hsp70).
Methods. Two models of tumor repopulation were employed, based on minimal population of cancer cells and application of conditioned medium (CM). To deplete the CMs of Hsp70 affinity chromatography on ATP-agarose and immunoprecipitation were used. Cell proliferation and the dynamics of cell growth were measured using MTT assay and xCELLigence technology; cell growth markers were estimated using qPCR and with the aid of ELISA for prostaglandin E detection. Immunoprecipitation followed by mass-spectrometry was employed to identify Hsp70-binding proteins and protein-protein interaction assays were developed to reveal the above protein complexes.
Results. It was found that CM of dying tumor cells contains tumor regrowth-initiating factors and the removal of one of them, Hsp70, caused a reduction in the relapse-activating capacity. The pull out of Hsp70 alone using ATP-agarose had no effect on repopulation, while the immunodepletion of Hsp70 dramatically reduced its repopulation activity. Using proteomic and immunochemical approaches, we showed that Hsp70 in conditioned medium binds and binds another abundant alarmin, the High Mobility Group B1 (HMGB1) protein; the complex is formed in tumor cells treated with anti-cancer drugs, persists in the cytosol and is further released from dying tumor cells. Recurrence-activating power of Hsp70-HMGB1 complex was proved by the enhanced expression of proliferation markers, Ki67, Aurka and MCM-10 as well as by increase of prostaglandin E production and autophagy activation. Accordingly, dissociating the complex with Hsp70 chaperone inhibitors significantly inhibited the pro-growth effects of the above complex, in both in vitro and in vivo tumor relapse models.
Conclusions. These data led us to suggest that the abundance of the Hsp70-HMGB1 complex in the extracellular matrix may serve as a novel marker of relapse state in cancer patients, while specific targeting of the complex may be promising in the treatment of cancers with a high risk of recurrence.
What Effect on User Behavior Do Decorative or Symbolic Designs on Traditional Ghanaian and Chinese Seats Have?
Authors: Appiah-Kubi Obed Persie , Wu Zhihui , Asamoah Obed Atta , Feng Xinhao 
Published: May 2023

Abstract
Traditional decorations, symbolic designs, or motifs on traditional seats do not only hold aesthetic values but also provide a conduit for expressing cultural philosophies and beliefs. In most Ghanaian and Chinese homes, seats meant for interior use are adorned with unique traditional symbols or decorative designs that continue to serve various domestic purposes. One unique value of traditional seats which needs to be documented is the effect and correlation between traditional symbols or decorative designs engineered in the seat and the behavior of people who continually use these artifacts. Using a mixed-methods approach, the study used a survey design. The data was sourced using interviews and questionnaires. 40 respondents were interviewed while 491 traditional seat users also responded to the questionnaire. Thematic analysis was used in analyzing the qualitative bit of the study while a Pearson Moment Correlation Coefficient (PMCC) was used to compute the extent of correlation between conveyed traditional symbols or decorative designs and the seat users’ behavior. Traditional decorative designs such as Gye Nyame, Sankofa, Akoma, Bi-nka-bi, akofena, Odo nnyew fie kwan, Hye Wonhye, Nyansapo, adinkrahene, Nkyinkyim, Mmere Dane, etc. which are associated with Ghanaians, and traditional Chinese decorative designs such as the bat, dragon, Moiré, eagle, elephant, the eight immortals, lotus flower, Kylin pattern, Babao pattern, ox, tiger, Buddha, etc. respectively conveyed on traditional Ghanaian and Chinese seat products negatively, neutrally, or positively affect the behavior of users of the seat (r = .4769, p = .00). Spiritually, the symbols or designs impact their prayer life, and their faith, and affect their fortune and luck. The feeling of comfort, courage, calmness, being optimistic, and being cheerful was the psychological impact of decorative designs. Culturally, users of traditional seat-bearing decorative designs or symbolic designs have become more tolerant, patient, chaste, honest, and loyal while they have also been assertive and unrelenting. Psychologically, it affects users’ comfort, courage, calmness, being optimistic, and being cheerful, while users’ personal development may also be affected leading to an automatic correlation with behavior. It was recommended that people should be encouraged to use traditional seats with traditional decorations or symbols that have positive meanings to help shape their lives in a positive direction.
WITHDRAWN: The Genetic Structure and Marker- Morphological Trait Associations in Forage Kochia Population Grown in Central Anatolia
Authors: KOYUN Nur KOÇ , HAKKI Erdoğan E. , ACAR Ramazan 
Published: May 2023

Abstract
Forage kochia, a naturally growing and semi-shrub in Türkiye's flora, tolerates adverse soil and climatic conditions. In the research, the morphological and yield values ​​of the forage kochia populations collected from 5 different locations in Konya were examined during 2018–2019. According to morphological and yield values, we determined 80 plants, 76 plants with excellent yield potential, and four outgroup plants for molecular studies. A total of 250 polymorphic fragments were obtained from these 80 plants. In our study, the average PIC value was 0.322, and the mean MI value was 8.99. Genetic diversity parameters of the populations were obtained using the GenAlEx program, and it was found that the mean He was 0.209, and the percentage of polymorphic loci was 81.20%. According to the results of AMOVA, among-population variation was 9%, while within-population variation was 91%. The dendrogram obtained as a result of the study determined that the genetic distance between plants varied between 0.63 and 0.90. According to the similarity index used in the study, it was stated that there was a high degree of similarity (90%) between 3212 and 5419 coded plants. Furthermore, it was noted that the markers related with plant height were associated with canopy diameter, number of main branches, and leaf color. The results show us that these populations are a treasured gene resource for plant breeding.
A Supervised Learning Regression method for the analysis of oral sensitivity of healthy subjects and patients with chemosensory loss
Authors: Naciri Lala Chaimae , Mastinu Mariano , Melis Melania , Green Tomer , Wolf Anne , Hummel Thomas , Barbarossa Iole Tomassini 
Published: May 2023

Abstract
The gustatory, olfactory, and trigeminal systems are anatomically separated. However, they interact cognitively to give rise to oral perception, which can significantly affect health and quality of life. We built a Supervised Learning (SL) regression model that, exploiting subjects’ features, was capable of automatically analyzing with high precision the self-ratings of oral sensitivity of healthy participants and patients with chemosensory loss, determining the contribution of its three components: gustatory, olfactory, and trigeminal. CatBoost regressor provided predicted values of the self-rated oral sensitivity close to experimental values. Patients showed lower predicted values of oral sensitivity, lower scores for measured taste, spiciness, astringency, and smell sensitivity, higher BMI, and lower levels of well-being. CatBoost regressor defined the impact of the single components of oral perception in the two groups. The trigeminal component was the most significant, though astringency and spiciness provided similar contributions in controls, while astringency was most important in patients. Taste was more important in controls while smell was the least important in both groups. Identification of the significance of the oral perception components and the differences found between the two groups provide important information to allow for more targeted examinations supporting both patients and healthcare professionals in clinical practice.
Neurological Outcome in Children Post–Sildenafil Therapy for Persistent Pulmonary Hypertension of the Newborn
Authors: Parapurath Rajiv , DM Madan Samuel 
Published: May 2023

Abstract
Purpose: Evaluation of neurological outcome post sildenafil and inhaled nitric oxide (iNO) therapy in the first 72-months of life.
Material and Methods: Prospective linear study of 84-neonates with severe persistent pulmonary hypertension of the newborn (PPHN). All neonates were on high frequency oscillatory ventilation (HFOV), surfactant, and inotropic support. In 40-neonates iNO was utilized, and intragastric sildenafil in 44-newborns. Bayley IIIUK was used at 38-months of age for developmental assessment. Intellectual ability was evaluated at 72-months of age by Wechsler Intelligence Scale for Children-Fourth EditionUK.
Results: iNO monotherapy had a mortality rate of 4% versus 0% on sildenafil, p = 0.001. Resolution of PPHN without sequelae occurred in 81%, iNO 68% versus 93% sildenafil, p = 0.0001. Resolution with neurological impairment ensued in 19%, iNO 32% versus 7% on sildenafil, p = 0.0001. Resolution with chronic lung disease was observed in 5%, iNO 8% versus 2% sildenafil, p = 0.020. Intragastric sildenafil monotherapy failure rate was 14%. Sildenafil was the predominant variable that reduced mortality, p = 0.02, CI: 0.010 – 0.080. At 38-months, Bayley IIIUK scores were normal ≥85 in 81%. Neurologically normal children at 72-months of age scored either average or high average, qualitatively, on the Wechsler Intelligence Scale for Children-Fourth EditionUK. Effect of sildenafil in the neonatal period was global leading to preserved intellectual, cognitive, and neurological outcome, in later childhood.
Conclusions: Intragastric sildenafil can be safely and effectively used in neonates with severe PPHN on HFOV and exogenous surfactant. Effect of sildenafil on neurodevelopment was positive.
Effects of dietary zinc on growth performance, digestive enzyme activities, antioxidant status and immune responses of sea cucumber Apostichopus japonicus
Authors: Xu Yuling , Gao Qinfeng , Dong Shuanglin , Hou Zhishuai , Mei Yaoping , Li Xueqi , Dong Kang , Li Zhao 
Published: May 2023

Abstract
This study evaluated the effects of dietary zinc on growth performance, digestive enzyme activities, antioxidant status and immune responses of sea cucumber Apostichopus japonicus. Five experimental diets were formulated with graded levels of zinc (0, 20, 40, 60, and 80 mg/kg, respectively), and the actual dietary zinc values were 31.4, 51.0, 68.2, 91.9, and 110.8 mg/kg diet, respectively. Sea cucumbers were fed with diets for 2 months. The results showed the growth performance, amylase and trypsin activities of sea cucumber increased significantly with zinc supplementation, and the best growth performance and enzyme activities were observed at 40 mg/kg zinc diet. Zinc supplementation significantly increased activities of superoxide dismutase (SOD), catalase (CAT), anti-superoxide anion (ASA) and inhibiting hydroxyl radical (AHR), while significantly reduced the malondialdehyde (MAD) content. Furthermore, the higher Zn supplementation levels resulted in significantly upregulated immune-related genes of hsp90、p105、rel、lsz, suggesting that excessive zinc caused oxidative stress. The broken-line regression analysis of specific growth rate (SGR) indicated dietary zinc requirement in sea cucumber was ~ 66.3 mg/kg diet.
The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody-drug conjugate–resistant HER2-overexpressing breast cancer
Authors: Lee Jangsoon , Kida Kumiko , Liu Huey , Manyam Ganiraju C. , Gi Young Jin , Multani Asha S. , Wang Jing , Jayachandran Gitanjali , Reuben James M. , Huo Lei , Tripathy Debu , Ueno Naoto T. 
Published: May 2023

Abstract

Background: Anti-HER2 therapies, including the HER2 antibody-drug conjugates trastuzumab emtansine (T-DM1) and trastuzumab deruxtecan (T-DXd), have improved survival outcomes for patients with HER2-overexpressing/amplified (HER2+) metastatic breast cancer. However, intrinsic or acquired resistance to anti-HER2–based therapies remains a clinical challenge for HER2+ metastatic breast cancer, as there is no standard of care following progression on T-DXd. The purpose of this study is to elucidate the mechanisms of resistance to the anti-HER2 antibody-drug conjugate (HER2-directed ADC) and identify targets whose inhibition enhances the efficacy of T-DXd in HER2-directed ADC–refractory HER2+ breast cancer.
Methods: Next-generation sequencing was performed to investigate genetic aberrations that arose after anti-HER2 therapy in 10 breast cancer patient samples. We generated T-DM1 and T-DXd–resistant HER2+ breast cancer cell lines. To elucidate their resistance mechanisms and to identify potential synergistic kinase targets for enhancing the efficacy of T-DXd, we used fluorescence in situhybridization, droplet digital PCR, Western blotting, whole-genome sequencing, cDNA microarray, and synthetic lethal kinome RNA interference screening. In addition, cell viability, colony formation, and xenograft assays were used to determine the synergistic antitumor effect of T-DXd combinations.
Results: We found reduced HER2 expression in 4 patients and amplified DNA repair–related genes in 4 patients after anti-HER2 therapy compared to levels before anti-HER2 therapy. We observed reduced ERBB2gene amplification in all HER2-directed ADC–resistant HER2+ breast cancer cell lines through DNA damage and epigenetic mechanisms. In HER2-directed ADC–resistant HER2+ breast cancer cell lines, pathway analysis identified the DNA repair pathway as a potential target canonical pathway to enhance the efficacy of T-DXd. The combination of T-DXd with ATR inhibitor BAY 1895344 led to significant breast cancer cell death in vitro (P<0.01) and in vivo (P<0.01) compared to single agents.
Conclusions: The DNA repair pathways may contribute to HER2-directed ADC resistance. Our data justify further exploring the combination of T-DXd with DNA repair–targeting drugs to treat HER2-directed ADC–resistant HER2+ breast cancer.