bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of superficial inferior epigastric vein superdrainage in TRAM and DIEP flap: An Indocyaning green angiography study of 68 cases
Authors: Zhu Ying , Yoo Hyokyung , Min Sally , Chang Hak 
Published: January 2023

Abstract
This study aimed to evaluate the efficacy of venous augmentation using superficial inferior epigastric vein (SIEV) in transverse rectus abdominis musculocutaneous (TRAM) and deep inferior epigastric artery perforator (DIEP) flap. A retrospective review was performed of 62 free TRAM and 6 DIEP unilateral breast reconstructions from September 2017 to July 2022. Intraoperative indocyanine green angiography was performed with the SIEV contralateral to the pedicle clamped and declamped for 20 min. The ratio of hypoperfused area was calculated and compared quantitatively. The preoperative computed tomography angiography was reviewed to measure the SIEV diameter and number of midline-crossing medial branches. Sixty-two percent (42/68 cases) resulted in perfusion improvement after SIEV superdrainage (Group 1), whereas 29.4 percent (20/68 cases) resulted in sustained (Group 2) and 8.8 percent (6/68 cases) in aggravated perfusion (Group3). The mean number of midline-crossing branches (p = 0.002) and mean difference in the diameter of bilateral SIEVs (p = 0.039) were significantly greater in Group 1 compared to the other groups. Superdrainage using the contralateral SIEV in TRAM/DIEP flap is recommended when there are more than 2 midline-crossing medial branches of SIEV and when the caliber of draining vein is greater than that of the pedicle side.
Comparison of time courses in postoperative functional outcomes between simultaneous bilateral and unilateral total knee arthroplasty with propensity score matching
Authors: Oka Tomohiro , Wada Osamu , Mizuno Kiyonori 
Published: January 2023

Abstract
Background: No consensus has been reached on which is better in terms of functional outcomes between simultaneous bilateral and unilateral total knee arthroplasty (TKA). As patient characteristics, such as age, sex, and body mass index, have significant effects on functional outcomes after TKA, we should match these factors before making comparisons. This study aimed to compare time courses in functional outcomes between simultaneous bilateral TKA and unilateral TKA after matching the patient characteristics.
Methods: In this retrospective study, we reviewed the clinical records of patients admitted to our hospital. After excluding the patients who did not meet the criteria, 43 underwent simultaneous bilateral TKA and 382 underwent unilateral TKA were included in this study. We performed propensity score matching for age, sex, and body mass index between simultaneous bilateral and unilateral TKA patients. We measured pain intensity, knee extensor strength, and knee-specific functional outcomes using the new knee society score, including total score, symptoms, patient satisfaction, patient expectations, and functional activities preoperatively and at 3 and 12 months postoperatively. Two-way repeated analysis of variance was performed to compare the time courses in functional outcomes between simultaneous bilateral and unilateral TKA.
Results: After propensity score matching, 40 patients each for underwent bilateral TKA and unilateral TKA were selected. Knee extensor strength in simultaneous bilateral TKA patients was significantly lower than that in unilateral TKA patients at 3 months postoperatively (p = 0.04). A significant interaction was observed between the effects of time and group on knee extensor strength (F [1, 78] = 3.338; interaction: p = 0.042; η2 = 0.052). No significant interactions were found among the other variables measured.
Conclusions: Patients who underwent simultaneous bilateral TKA showed delayed recovery in knee extensor strength at 3 months postoperatively, but recovered to the same level at 12 months postoperatively. Patients who underwent simultaneous bilateral TKA should undergo postoperative rehabilitation focused on alleviating delayed recovery in knee extensor strength during the acute phase.
Integration of Transcriptome and Metabolome Provides Unique Insights to Pathways Associated with Wheat Immature Embryos Infected by Agrobacterium tumefaciens
Authors: Weiwei Wang , Jinliang Guo , Jiayang Ma , Zhulin Wang , Lining Zhang , Zixu Wang , Chao Zhang , Fengli Sun , Yajun Xi 
Published: January 2023

AbstractBackground:The transformation efficiency (TE) of wheat is slow due to its recalcitrance nature to in vitro regeneration and genetic transformation. Nowadays, the TE of wheat immature embryo from cultivar Fielder in the PureWheat technique was greatly improved by a series of special chemical and physical methods. To explore the mechanisms of wheat high TE in this protocol, we strictly followed the procedures to obtain wheat immature embryos afterAgrobacteriuminfection for 24 and 48 h, observed the surface of immature embryos using scanning electron microscope (SEM), and conducted comprehensive transcriptome and metabolome analysis.Results:SEM showed thatAgrobacterium tumefacienswere deposited under the damaged cortex of wheat immature embryos caused by pretreatment and contacted the receptor cells to improve the TE. Transcriptome analysis showed that the differentially expressed genes were mainly enriched in phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant-pathogen interaction, plant hormone signal transduction, MAPK signaling pathway. By analyzing the correlation between differentially expressed genes and metabolites, the expression of many genes and accumulation of metabolites are changed in glucose metabolism and the TCA cycle, as well as amino acid metabolism, it suggests that the wheat embryo infected withAgrobacteriumis an energy demanding process. The shikimate pathway may act as a hub between glucose metabolism and phenylpropane metabolism duringAgrobacteriuminfection. The down-regulation of F5H gene and up-regulation of CCR gene led to the accumulation of lignin precursor through phenylpropane metabolism. In addition, several metabolic pathways and oxidases were found to be involved in the infection treatment, including melatonin biosynthesis, benzoxazinoid biosynthesis, betaine biosynthesis, superoxide dismutase, peroxidase, which suggest that wheat embryo may be under the stress ofAgrobacteriumand thus undergo oxidative stress response.Conclusion:Our findings contribute to understand the underlying the mysterious mechanism of the high TE of wheat immature embryos stimulated byAgrobacteriumby exploring valuable genes and metabolites.
Analysis of influencing factors of small airway dysfunction in adults
Authors: Zhang Yifan , Zhang Haihua , Su Xuan , Wang Ying , Gao Guizhou , Wang Xiaodong , Zhang Tao 
Published: January 2023

Abstract
Background
Small airway dysfunction (SAD) is a widespread, but less typical clinical manifestation of respiratory dysfunction. In lung diseases, SAD can have a higher-than-expected impact on lung function. The aim of this study was to understand the factors that contribute to SAD.
Methods
We included 1233 patients in the pulmonary function room of TangDu Hospital from June 2021 to December 2021. Functional examination guidelines diagnostic criteria: MMEF, MEF50%, MEF25%, two of the three items are lower than 65% of the predicted value, the patient was diagnosed with SAD. We divided the subjects into a small airway disorder group and non-small airway disorder group, and they were asked to complete a questionnaire, and we performed a multivariate scoring of the relevant factors after univariate comparisons among other factors.
Results
The influencing factors of patients with small airway disorder were advanced age (OR = 7.772,95% CI 2.284–26.443), female (OR = 1.545,95% CI 1.103–2.164), family history of respiratory disease (OR = 1.508,95% CI 1.069–2.126), history of occupational dust exposure (OR = 1.723,95% CI 1.177–2.521), history of smoking (OR = 1.732,95% CI 1.231–2.436), history of pet exposure (OR = 1.499,95% CI 1.065–2.110), exposure to O3(OR = 1.008,95% CI 1.003–1.013), chronic bronchitis (OR = 1.947,95% CI 1.376–2.753), emphysema (OR = 2.190,95% CI 1.355–3.539) and asthma (OR = 7.287,95% CI 3.546–14.973).
Conclusion
Small airway disorders are associated with age, gender, exposure to O3, family history of respiratory disease, occupational dust exposure, smoking history, history of pet exposure, chronic bronchitis, and asthma. People with these risk factors should take appropriate precautions to prevent SAD.
Analysis of the influencing factors related to spasticity in patients with spinal cord injuries: a retrospective study
Authors: Wu Shuang , Song Ning , Deng Luoyi , Wang Jia , Wang Xianbin 
Published: January 2023

Abstract
STUDY DESIGN: a retrospective study
OBJECTIVES: The aim of this study was to investigate the related influencing factors of spasticity in individuals with spinal cord injury (SCI).
SETTING: Individuals diagnosed with SCI between January 2019 and December 2021 in the Department of Rehabilitation Medicine, Affiliated Hospital of Guizhou Medical University.
METHODS:
Individuals diagnosed with SCI between January 2019 and December 2021 in the Department of Rehabilitation Medicine, Affiliated Hospital of Guizhou Medical University, were screened for spasticity by using the modified Ashworth spasm scale. A total of 183 individuals diagnosed with SCI were finally included in the study. We collected the individuals’ basic information, including gender, age, disease course, injury segment, American Spinal Injury Association (ASIA) grade, Cause of injury. Univariate and dichotomous logistic regression analysis analyses were used to examine the influencing factors of spasticity in the individuals with SCI.
RESULTS:
The chi-squared test revealed that the course of the disease (p=0.004), the injured segment (p<0.001) and the pressure ulcers of the individuals (p=0.002) with SCI had statistical significance. A dichotomous logistic regression analysis was performed to analyze the influencing factors of spasticity. the course of the disease, the injured segment and the pressure ulcers of the individuals with SCI were independent influencing factors of spasticity in individuals with SCI, among which the prolongation of the course of the disease (when the course of disease was 3 to 6 months and 6 months or more, the incidence of spasticity was 2.441 times (95% CI, 0.968~6.157) and 5.090 times (95% CI, 1.883~13.76), compared with a disease course of less than 3 months) and the presence of pressure ulcers were independent risk factors (the incidence of spasticity in patients with pressure ulcers was 2.481 times (95% CI, 0.961~6.406) higher than that without pressure ulcers). the low injury segment was a protective factor (the incidence of spasticity in thoracic and lumbar spinal cord injuries was 0.363 times (95% CI, 0.154~0.855) and 0.034 times (95% CI, 0.004~0.268) higher than that in cervical spinal cord injuries).
CONCLUSION:
In this study, we found that there was no significant correlation between spasticity after SCI and gender, ASIA grade, cause of injury and other factors. However, the prolongation of the course of the disease and the presence of Pressure ulcers were independent risk factors, and the low injury segment was a protective factor.
Intravoxel incoherent motion (IVIM) for distinguishing glioma and solitary brain metastasis: A pilot multi-parameter magnetic resonance imaging histogram and texture analysis
Authors: Su Yifei , Cheng Rui , Guo Jinxia , Zhang Miaoqi , Wang Junhao , Wang Chunhong , Ji Hongming , Hao Liangliang , He Yexin , Xu Cheng 
Published: January 2023

Abstract
Background: To investigate the value of diffusion-weighted imaging (DWI), intravoxel incoherent motion (IVIM), and amide proton transfer-weighted (APTW) imaging in differentiating the primary glioma from solitary brain metastases (SBM) with the histogram and texture features.
Methods: A total of 65 patients with newly diagnosed glioma or metastases were enrolled. All patients underwent DWI, IVIM, and APTW imaging, as well as the T1W, T2W, T2FLAIR, and enhanced T1W imaging. The first-order texture and histogram features of apparent diffusion coefficient (ADC) from DWI, diffusion coefficient (D), perfusion fraction (f), pseudo-diffusion coefficient (D*) from IVIM, and MTRasym (3.5 ppm) from APTW were extracted from the tumor parenchyma and compared between glioma and SBM. Parameters with significant differences were analyzed with the logistics regression and receiver operator curves to explore the optimal model and compare the differentiation performance.
Results: Higher ADCkurtosis (p=0.02), f90 (p＜0.01), and fkurtosis (p=0.01) were found for glioma, while higher MTRasym (3.5ppm)10 (p= 0.05), fentropy (p＜0.01), and fmean (p＜0.01) were observed for SBM. fkurtosis (OR =0.66, 95%CI 0.48-0.92, p=0.02), and fmean (OR=1.44, 95%CI 1.16-1.18, p<0.01) were independent factors for SBM differentiation. The model combining age, fkurtosis, and fmean showed an AUC of 0.83, while the model combined with fkurtosis and fmean with improved sensitivity had an AUC of 0.81.
Conclusion: The fmean and fkurtosis in enhanced tumor region could be used to differentiate glioma and SBM.
Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity
Authors: Mukai Yasutaka , Li Yan , Nakamura Akiyo , Fukatsu Noriaki , Iijima Daisuke , Abe Manabu , Sakimura Kenji , Itoi Keiichi , Yamanaka Akihiro 
Published: January 2023

Abstract
Optogenetics is a powerful tool for manipulating neuronal activity by light illumination with high temporal and spatial resolution. Anion-channelrhodopsins (ACRs) are light-gated anion channels that allow researchers to efficiently inhibit neuronal activity. A blue light-sensitive ACR2 has recently been used in several in vivo studies; however, the reporter mouse strain expressing ACR2 has not yet been reported. Here, we generated a new reporter mouse strain, LSL-ACR2, in which ACR2 is expressed under the control of Cre recombinase. We crossed this strain with a noradrenergic neuron-specific driver mouse (NAT-Cre) to generate NAT-ACR2 mice. We confirmed Cre-dependent expression and function of ACR2 in the targeted neurons by immunohistochemistry and electrophysiological recordings in vitro, and confirmed physiological function using an in vivo behavioral experiment. Our results show that the LSL-ACR2 mouse strain can be applied for optogenetic inhibition of targeted neurons, particularly for long-lasting continuous inhibition, upon crossing with Cre-driver mouse strains. The LSL-ACR2 strain can be used to prepare transgenic mice with homogenous expression of ACR2 in targeted neurons with a high penetration ratio, good reproducibility, and no tissue invasion.
Self-reported musculoskeletal disorders and associated factors among HIV/AIDS patients following ART at University Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia, 2021
Authors: Kibret Alemu Kassaw , Mekie Melisew , Eriku Getachew Azeze , Gashaw Moges , Yalew Ermias Solomon , Weldetsadik Fkrte Kebede 
Published: January 2023

Abstract
Background: People with HIV/AIDS following ART experience immunological improvement and normal life expectancy. However, with this increased life expectancy has emerged a symptom burden such as musculoskeletal pain/disorder. Despite, this health problems in HIV/AIDS patients (PLWHAs) have become a concern, there is a lack of data on the prevalence and associated factors of musculoskeletal disorders among HIV/AIDS following ART in sub-Saharan Africa and also in Ethiopia. Therefore, the aim of this study was to assess the prevalence and associated factors of musculoskeletal disorders among adult HIV/AIDS patients following ART clinic at University of Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia 2021.
Method: An institutional-based cross-sectional study was conducted from September 1st to October 1st, 2021 at University of Gondar comprehensive specialized hospital, Gondar, Ethiopia. The data was collected through an interview-administered questionnaire and patient medical record review of 324 participants. Binary logistic regression was used to identify associated risk factors of musculoskeletal disorders. The strength of the association was detected by the adjusted odds ratio and P-value.
Result: A total of 324, with nearly half the age range of 25-44 years participants were enrolled in this study. Among participants, 158 (48.5%) of them experienced musculoskeletal disorders of annual prevalence. BMI, opportunistic infection [AOR, 10.43; 95%CI 2.756-42.25], type of ART medication used, CD4-count [AOR, 0.13; 95% CI 0.032-0.852], and change in ART regimen change [AOR, 8.14; 95%CI 2.062-32.09] were significantly associated with a musculoskeletal disorder.
Conclusion: The prevalence of musculoskeletal disorders was moderate (48.5%). BMI, recent CD4 count, opportunistic infection, ART regime at initiation, and ART regime change were significantly associated with a musculoskeletal disorder. A multidisciplinary approach is required, to link the ART clinic and physiotherapy department for preventing and treating musculoskeletal disorders among HIV/AIDS patients following ART.
A Clinical Prediction Model to Predict albumin Treatment Outcomes and Provide Dosage Recommendations: Development and Validation Study
Authors: Su Bo , li hui 
Published: January 2023

Abstract
Objective: Intravenous Albumin is widely used in the intensive care unit in patients with Systemic lupus erythematosus. However, The dose of albumin is still controversial.In this study, we intended to develop and validate a machine learning–based model to predict intravenous albumin treatment outcomes and to provide dosage recommendations to clinicians.
Methods: Using Mimic-III V1.4 and Mimic-IV V0.4, we collected data from of patients with SLE admitted to the ICU. Data were divided into two groups based on death or survival within 30 days.Several machine learning methods were used to build prediction models, evaluating model performance by the model area under the receiver operating characteristic curve(AUC).The best performing prediction model was selected. Applying the model to make predictions. The value of intravenous albumin infusion in the input variable was changed using the actual albumin used by the clinician as the baseline. Find the dose that minimizes the risk of death.
Results: Mimic-iv and Mimic-III was used to establish and validation the model. A total of 427 SLE patients, 109 of whom died. They were divided into two groups according to whether they died within 30 days. Random subspace method(RSM) integrated linear discriminant analysis model(LDA) were established. In the external validation AUC results of the models were 0.79[95% CI 0.78 0.82],the model performance was the best. Predictors were: Age at admission in years, Minimum WBC, Maximum WBC, Mechanical ventilation, Mean heart rate, Minimum systolic BP, Vasopressor, dialysis, Accompanied hypertension, nterstitial pneumonia associated with systemic lupus erythematosus, sofa, sirs, sapsii, apsiii, Intravenous administration of albumin, Immunoglobulin M, Minimum Albumin, Urine volume on the first day after admission to ICU, Infection, Complement C4.The model was used to calculate the recommended dose of albumin for intravenous infusion. In the MIMICIV database:366 patients in the survival group, 73 cases in the death group. The survival group improved 26 cases after applying our recommended dose, In the death group, 30 cases were improved. In the MIMICIII database:61 patients in the survival group,36 cases in the death group. The survival group improved 1 cases after applying our recommended dose, In the death group, 6 cases were improved.
Conclusions: RSM-LDA model could be used in the clinical scenario of albumin was infused intravenously treatment with a dosage pattern. The results provide support for recommending optimal albumin was infused intravenously treatment dosing to clinicians.
Effect of Patient-Centred Communication and Usual Source of Care on Potentially Preventable Hospitalization among Patients with Chronic Diseases and Disabilities
Authors: Choi Hyoungshim , Yoon Hyejung , Jung Young-il 
Published: January 2023

Abstract
Background: This study examined the effect of the degree of patient-centred communication (PCC) and having a usual source of care (USC) on potentially preventable hospitalization (PPH) among chronic disease patients with disability. In particular, we empirically analysed whether PCC and USC could reduce the possibility of PPH among patients with disabilities compared to that among those without disabilities.
Methods: This study used data from the Korean Health Panel Survey 2017-2018 (7,271 patients without disabilities and 774 patients with disabilities). The dependent variable was extracted from the 2018 KHPS data, while all other variables included in the analysis were extracted from the 2017 KHPS data and subjected to multivariate logistic regression analysis; subgroup analysis was performed according to the severity of disability.
Results: The incidence of PPH was higher in the group with disabilities (4.5%) than in the group without disabilities (2.2%). Significantly more patients with disabilities than those without disabilities had a USC (67.7% vs. 56.9%), and more patients with severe disabilities than those with mild disabilities had a USC (75.9% vs. 63.5%). Multivariate logistic regression analysis showed that the risk of PPH increased when PCC was insufficient in patients with disabilities (OR = 2.92, 95%CI [1.41–6.04]), those with mild disabilities (OR = 2.51, 95%CI [1.02–6.20]), and those in the severe disability group who did not have a USC (OR = 5.06, 95%CI [1.03–24.80]). The effects of USCs and PCC on PPH were not observed in patients without disabilities.
Conclusion: Sufficient PCC and having a USC helped reduce the incidence of PPH among patients with disabilities and chronic conditions. It is necessary to find practical ways to enhance PCC and expand the use of USCs among patients with disabilities so that they can receive appropriate primary healthcare services in a timely manner.
Synthesis and physicochemical characterization of acyl myricetins as potential anti-neuroexocytotic agents
Authors: Cho Sora , Kong Byoungjae , Jung Younghun , Shin Jonghyeok , Park Myungseo , Chung Woo-Jae , Ban Choongjin , Kweon Dae-Hyuk 
Published: January 2023

Abstract
Acyl myricetins (monopropionyl-, dipropionyl-, and monooctanoyl-myricetin, termed as MP1, MP2, and MO1, respectively) were synthesized through enzymatic or non-enzymatic esterification reaction of myricetin aglycone. Structure study indicated the hydroxyl group at C4ʹ in B-ring was highly susceptible to acylation. Over its parental myricetin, acylated compounds showed enhanced lipophilicity (from 7.4- to 26.3-fold) and oxidative stability (from 1.9- to 3.1-fold) on the basis of logP and decay rate, respectively. MO1, presenting the physicochemical superiority compared to the others, provided lowest EC50 value of 2.51 µM on inhibition of neutrotransmitter release and high CC50 value of 58.96 µM, leading to widest therapeutic window. All myricetin esters did not show any irritation toxicity when assessed with a chicken embryo assay. This study describes information on acylation of myricetin that has not yet been explored, and suggests that MO1 has anti-neuroexocytotic potential for industrial application due to its enhanced biological properties.