bluuebunny/crossref_metadata_2025_split huggingface dataset:
What do women with sexual interest in children tell us about the assumed cause of their sexual interest in children, (non-)disclosure, and professional help?—Results of a qualitative content analysis
Authors: Tozdan Safiye , Hübener Greta , Briken Peer , Schröder Johanna 
Published: March 2023

AbstractResearch on women with sexual interest in children is still rare, especially regarding women’s own theories about the cause of their sexual interest in children, their experiences with (non-)disclosure, and professional help. In the context of a broader online study, we provided 50 women with a sexual interest in children under the age of 14 years (mean age: 33.6, SD = 11.1) with open questions regarding their own theories about what causes their sexual interest in children, experiences with disclosure and non-disclosure, and experiences with and opinions about professional help. Analyses were conducted using an inductive qualitative content analysis method that aimed at ordering and structuring manifest and latent content by categorizing qualitative data. Results revealed that participants mainly think that past experiences caused their sexual interest in children (Σ = 16), e.g., abusive or non-abusive sexual experiences during childhood. Some participants think that their sexual interest in children is a disposition they were born with (Σ = 8). Disclose of sexual interest in children to another person was reported by 56.0% of the present sample and led to rather positive consequences (Σ = 24, e.g., acceptance or support). Those who did not disclose (44.0%) mainly did so due to fear of rejection and/or stigmatization (Σ = 24). A total of 30.0% already sought help due to their sexual interest in children and frequently reported negative experiences (Σ = 15). A frequent statement participants made on how to reach women with sexual interest in children in order to offer professional help was the destigmatization of sexual interest in children (Σ = 14). We recommend that women with sexual interest in children should be taken more seriously among research and in prevention measures.
Neuro-computational mechanisms and individual biases in action-outcome learning under moral conflict
Authors: Fornari Laura , Ioumpa Kalliopi , Nostro Alessandra D. , Evans Nathan J. , De Angelis Lorenzo , Speer Sebastian P. H. , Paracampo Riccardo , Gallo Selene , Spezio Michael , Keysers Christian , Gazzola Valeria 
Published: March 2023

AbstractLearning to predict action outcomes in morally conflicting situations is essential for social decision-making but poorly understood. Here we tested which forms of Reinforcement Learning Theory capture how participants learn to choose between self-money and other-shocks, and how they adapt to changes in contingencies. We find choices were better described by a reinforcement learning model based on the current value of separately expected outcomes than by one based on the combined historical values of past outcomes. Participants track expected values of self-money and other-shocks separately, with the substantial individual difference in preference reflected in a valuation parameter balancing their relative weight. This valuation parameter also predicted choices in an independent costly helping task. The expectations of self-money and other-shocks were biased toward the favored outcome but fMRI revealed this bias to be reflected in the ventromedial prefrontal cortex while the pain-observation network represented pain prediction errors independently of individual preferences.
The crystal structure of a simian Foamy Virus receptor binding domain provides clues about entry into host cells
Authors: Fernández Ignacio , Dynesen Lasse Toftdal , Coquin Youna , Pederzoli Riccardo , Brun Delphine , Haouz Ahmed , Gessain Antoine , Rey Félix A. , Buseyne Florence , Backovic Marija 
Published: March 2023

AbstractThe surface envelope glycoprotein (Env) of all retroviruses mediates virus binding to cells and fusion of the viral and cellular membranes. A structure-function relationship for the HIV Env that belongs to the Orthoretrovirus subfamily has been well established. Structural information is however largely missing for the Env of Foamy viruses (FVs), the second retroviral subfamily. In this work we present the X-ray structure of the receptor binding domain (RBD) of a simian FV Env at 2.57 Å resolution, revealing two subdomains and an unprecedented fold. We have generated a model for the organization of the RBDs within the trimeric Env, which indicates that the upper subdomains form a cage-like structure at the apex of the Env, and identified residues K342, R343, R359 and R369 in the lower subdomain as key players for the interaction of the RBD and viral particles with heparan sulfate.
Serum/glucocorticoid-inducible kinase 1 deficiency induces NLRP3 inflammasome activation and autoinflammation of macrophages in a murine endolymphatic hydrops model
Authors: Zhang Dao-Gong , Yu Wen-Qian , Liu Jia-Hui , Kong Li-Gang , Zhang Na , Song Yong-Dong , Li Xiao-Fei , Fan Zhao-Min , Lyu Ya-Feng , Li Na , Wang Hai-Bo 
Published: March 2023

AbstractMénière’s disease, a multifactorial disorder of the inner ear, is characterized by severe vertigo episodes and hearing loss. Although the role of immune responses in Ménière’s disease has been proposed, the precise mechanisms remain undefined. Here, we show that downregulation of serum/glucocorticoid-inducible kinase 1 is associated with activation of NLRP3 inflammasome in vestibular-resident macrophage-like cells from Ménière’s disease patients. Serum/glucocorticoid-inducible kinase 1 depletion markedly enhances IL-1β production which leads to the damage of inner ear hair cells and vestibular nerve. Mechanistically, serum/glucocorticoid-inducible kinase 1 binds to the PYD domain of NLRP3 and phosphorylates it at Serine 5, thereby interfering inflammasome assembly. Sgk−/− mice show aggravated audiovestibular symptoms and enhanced inflammasome activation in lipopolysaccharide-induced endolymphatic hydrops model, which is ameliorated by blocking NLRP3. Pharmacological inhibition of serum/glucocorticoid-inducible kinase 1 increases the disease severity in vivo. Our studies demonstrate that serum/glucocorticoid-inducible kinase 1 functions as a physiologic inhibitor of NLRP3 inflammasome activation and maintains inner ear immune homeostasis, reciprocally participating in models of Ménière’s disease pathogenesis.
An intronic variant in TBX4 in a single family with variable and severe pulmonary manifestations
Authors: Flanagan Frances O. , Holtz Alexander M. , Vargas Sara O. , Genetti Casie A. , Schmitz-Abe Klaus , Casey Alicia , Kennedy John C. , Raby Benjamin A. , Mullen Mary P. , Fishman Martha P. , Agrawal Pankaj B. 
Published: March 2023

AbstractA male infant presented at term with neonatal respiratory failure and pulmonary hypertension. His respiratory symptoms improved initially, but he exhibited a biphasic clinical course, re-presenting at 15 months of age with tachypnea, interstitial lung disease, and progressive pulmonary hypertension. We identified an intronic TBX4 gene variant in close proximity to the canonical donor splice site of exon 3 (hg 19; chr17:59543302; c.401 + 3 A > T), also carried by his father who had a typical TBX4-associated skeletal phenotype and mild pulmonary hypertension, and by his deceased sister who died shortly after birth of acinar dysplasia. Analysis of patient-derived cells demonstrated a significant reduction in TBX4 expression resulting from this intronic variant. Our study illustrates the variable expressivity in cardiopulmonary phenotype conferred by TBX4 mutation and the utility of genetic diagnostics in enabling accurate identification and classification of more subtly affected family members.
Microfluidics-free single-cell genomics with templated emulsification
Authors: Clark Iain C. , Fontanez Kristina M. , Meltzer Robert H. , Xue Yi , Hayford Corey , May-Zhang Aaron , D’Amato Chris , Osman Ahmad , Zhang Jesse Q. , Hettige Pabodha , Ishibashi Jacob S. A. , Delley Cyrille L. , Weisgerber Daniel W. , Replogle Joseph M. , Jost Marco , Phong Kiet T. , Kennedy Vanessa E. , Peretz Cheryl A. C. , Kim Esther A. , Song Siyou , Karlon William , Weissman Jonathan S. , Smith Catherine C. , Gartner Zev J. , Abate Adam R. 
Published: March 2023

AbstractCurrent single-cell RNA-sequencing approaches have limitations that stem from the microfluidic devices or fluid handling steps required for sample processing. We develop a method that does not require specialized microfluidic devices, expertise or hardware. Our approach is based on particle-templated emulsification, which allows single-cell encapsulation and barcoding of cDNA in uniform droplet emulsions with only a vortexer. Particle-templated instant partition sequencing (PIP-seq) accommodates a wide range of emulsification formats, including microwell plates and large-volume conical tubes, enabling thousands of samples or millions of cells to be processed in minutes. We demonstrate that PIP-seq produces high-purity transcriptomes in mouse–human mixing studies, is compatible with multiomics measurements and can accurately characterize cell types in human breast tissue compared to a commercial microfluidic platform. Single-cell transcriptional profiling of mixed phenotype acute leukemia using PIP-seq reveals the emergence of heterogeneity within chemotherapy-resistant cell subsets that were hidden by standard immunophenotyping. PIP-seq is a simple, flexible and scalable next-generation workflow that extends single-cell sequencing to new applications.
High-resolution alignment of single-cell and spatial transcriptomes with CytoSPACE
Authors: Vahid Milad R. , Brown Erin L. , Steen Chloé B. , Zhang Wubing , Jeon Hyun Soo , Kang Minji , Gentles Andrew J. , Newman Aaron M. 
Published: March 2023

AbstractRecent studies have emphasized the importance of single-cell spatial biology, yet available assays for spatial transcriptomics have limited gene recovery or low spatial resolution. Here we introduce CytoSPACE, an optimization method for mapping individual cells from a single-cell RNA sequencing atlas to spatial expression profiles. Across diverse platforms and tissue types, we show that CytoSPACE outperforms previous methods with respect to noise tolerance and accuracy, enabling tissue cartography at single-cell resolution.
Evolution of synchronous female bilateral breast cancers and response to treatment
Authors: Hamy Anne-Sophie , Abécassis Judith , Driouch Keltouma , Darrigues Lauren , Vandenbogaert Mathias , Laurent Cecile , Zaccarini Francois , Sadacca Benjamin , Delomenie Myriam , Laas Enora , Mariani Odette , Lam Thanh , Grandal Beatriz , Laé Marick , Bieche Ivan , Vacher Sophie , Pierga Jean-Yves , Brain Etienne , Vallot Celine , Hotton Judicael , Richer Wilfrid , Rocha Dario , Tariq Zakia , Becette Veronique , Meseure Didier , Lesage Laetitia , Vincent-Salomon Anne , Filmann Natalie , Furlanetto Jenny , Loibl Sibylle , Dumas Elise , Waterfall Joshua J. , Reyal Fabien 
Published: March 2023

AbstractSynchronous bilateral breast cancer (sBBC) occurs after both breasts have been affected by the same germline genetics and environmental exposures. Little evidence exists regarding immune infiltration and response to treatment in sBBCs. Here we show that the impact of the subtype of breast cancer on levels of tumor infiltrating lymphocytes (TILs, n = 277) and on pathologic complete response (pCR) rates (n = 140) differed according to the concordant or discordant subtype of breast cancer of the contralateral tumor: luminal breast tumors with a discordant contralateral tumor had higher TIL levels and higher pCR rates than those with a concordant contralateral tumor. Tumor sequencing revealed that left and right tumors (n = 20) were independent regarding somatic mutations, copy number alterations and clonal phylogeny, whereas primary tumor and residual disease were closely related both from the somatic mutation and from the transcriptomic point of view. Our study indicates that tumor-intrinsic characteristics may have a role in the association of tumor immunity and pCR and demonstrates that the characteristics of the contralateral tumor are also associated with immune infiltration and response to treatment.
Grid-wise simulation acceleration of the electromagnetic fields of 2D optical devices using super-resolution
Authors: Seo Jangwon , Kim Insoo , Seok Junhee 
Published: March 2023

AbstractThe significance of simulation has been increasing in device design due to the cost of real test. The accuracy of the simulation increases as the resolution of the simulation increases. However, the high-resolution simulation is not suited for actual device design because the amount of computing exponentially increases as the resolution increases. In this study, we introduce a model that predicts high-resolution outcomes using low-resolution calculated values which successfully achieves high simulation accuracy with low computational cost. The fast residual learning super-resolution (FRSR) convolutional network model is a model that we introduced that can simulate electromagnetic fields of optical. Our model achieved high accuracy when using the super-resolution technique on a 2D slit array under specific circumstances and achieved an approximately 18 times faster execution time than the simulator. To reduce the model training time and enhance performance, the proposed model shows the best accuracy (R2: 0.9941) by restoring high-resolution images using residual learning and a post-upsampling method to reduce computation. It has the shortest training time among the models that use super-resolution (7000 s). This model addresses the issue of temporal limitations of high-resolution simulations of device module characteristics.
No impact of nitrogen fertilization on carbon sequestration in a temperate Pinus densiflora forest
Authors: Baek Gyeongwon , Lim Hyungwoo , Noh Nam Jin , Kim Choonsig 
Published: March 2023

AbstractCarbon (C) sequestration capacity in forest ecosystems is generally constrained by soil nitrogen (N) availability. Consequently, N fertilization is seen as a promising tool for enhancing ecosystem-level C sequestration in N-limited forests. We examined the responses of ecosystem C (vegetation and soil) and soil N dynamics to 3 years of annual nitrogen-phosphorus-potassium (N3P4K1 = 11.3 g N, 15.0 g P, 3.7 g K m−2 year−1) or PK fertilization (P4K1), observed over 4 years in a 40-year-old Pinus densiflora forest with poor N nutrition in South Korea. PK fertilization without N was performed to test for PK limitation other than N. Neither tree growth nor soil C fluxes responded to annual NPK or PK fertilization despite an increase in soil mineral N fluxes following NPK fertilization. NPK fertilization increased the rate of N immobilization and 80% of the added N was recovered from mineral soil in the 0–5 cm layer, suggesting that relatively little of the added N was available to trees. These results indicate that N fertilization does not always enhance C sequestration even in forests with poor N nutrition and should therefore be applied with caution.
Type 2 diabetes prevalence, awareness, and risk factors in rural Mali: a cross-sectional study
Authors: Diawara Abdoulaye , Coulibaly Djibril Mamadou , Hussain Talib Yusuf Abbas , Cisse Cheickna , Li Jian , Wele Mamadou , Diakite Mahamadou , Traore Kassim , Doumbia Seydou O. , Shaffer Jeffrey G. 
Published: March 2023

AbstractDiabetes is currently a crisis in sub-Saharan West Africa (SSWA) with dramatic implications for public health and national budgets prioritizing infectious diseases. There is limited recent literature about the prevalence, awareness, and risk factors for type 2 diabetes (T2D) in rural parts of SSWA. This study characterized T2D prevalence and risk factors for the rural Malian community of Nièna, which is situated in Mali’s second-largest province of Sikasso. Between December 2020 and July 2021, a cross-sectional study of 412 participants was conducted in the Nièna community using clinical questionnaires and rapid diagnostic tests. Among 412 participants, there were 143 (34.7%) and 269 (65.3%) males and females, respectively. The overall prevalence of T2D in Nièna was 7.5% (31/412), and prevalence rates were 8.6% (23/269) and 5.6% (8/143) for females and males, respectively. Age, family history of diabetes, hypertension, waist circumference, and fetal macrosomia were significantly associated with T2D (p = 0.007, p < 0.001, p = 0.003, p = 0.013, and p < 0.001, respectively). Notably, 61.3% (19/31) of T2D subjects were unaware of their diabetic status before the study. Field surveys have considerable utility in driving T2D awareness in rural African settings.
Data driven contagion risk management in low-income countries using machine learning applications with COVID-19 in South Asia
Authors: Shonchoy Abu S. , Mahzab Moogdho M. , Mahmood Towhid I. , Ali Manhal 
Published: March 2023

AbstractIn the absence of real-time surveillance data, it is difficult to derive an early warning system and potential outbreak locations with the existing epidemiological models, especially in resource-constrained countries. We proposed a contagion risk index (CR-Index)—based on publicly available national statistics—founded on communicable disease spreadability vectors. Utilizing the daily COVID-19 data (positive cases and deaths) from 2020 to 2022, we developed country-specific and sub-national CR-Index for South Asia (India, Pakistan, and Bangladesh) and identified potential infection hotspots—aiding policymakers with efficient mitigation planning. Across the study period, the week-by-week and fixed-effects regression estimates demonstrate a strong correlation between the proposed CR-Index and sub-national (district-level) COVID-19 statistics. We validated the CR-Index using machine learning methods by evaluating the out-of-sample predictive performance. Machine learning driven validation showed that the CR-Index can correctly predict districts with high incidents of COVID-19 cases and deaths more than 85% of the time. This proposed CR-Index is a simple, replicable, and easily interpretable tool that can help low-income countries prioritize resource mobilization to contain the disease spread and associated crisis management with global relevance and applicability. This index can also help to contain future pandemics (and epidemics) and manage their far-reaching adverse consequences.
Genetic structuring and invasion status of the perennial Ambrosia psilostachya (Asteraceae) in Europe
Authors: Karrer Gerhard , Hall Rea Maria , Le Corre Valérie , Kropf Matthias 
Published: March 2023

AbstractThe perennial western ragweed (Ambrosia psilostachya DC.) arrived from North America to Europe in the late nineteenth century and behaves invasive in its non-native range. Due to its efficient vegetative propagation via root suckers, A. psilostachya got naturalized in major parts of Europe forming extensive populations in Mediterranean coastal areas. The invasion history, the spreading process, the relationships among the populations as well as population structuring is not yet explored. This paper aims to give first insights into the population genetics of A. psilostachya in its non-native European range based on 60 sampled populations and 15 Simple Sequence Repeats (SSR). By AMOVA analysis we detected 10.4% of genetic variation occurring among (pre-defined) regions. These regions represent important harbors for trading goods from America to Europe that might have served as source for founder populations. Bayesian Clustering revealed that spatial distribution of genetic variation of populations is best explained by six groups, mainly corresponding to regions around important harbors. As northern populations show high degrees of clonality and lowest levels of within-population genetic diversity (mean Ho = 0.40 ± 0.09), they could preserve the initial genetic variation levels by long-lived clonal genets. In Mediterranean populations A. psilostachya expanded to millions of shoots. Some of those were obviously spread by sea current along the coast to new sites, where they initiated populations characterized by a lower genetic diversity. For the future, the invasion history in Europe might get clearer after consideration of North American source populations of western ragweed.
The influence of solar-modulated regional circulations and galactic cosmic rays on global cloud distribution
Authors: Kumar Vinay , Dhaka Surendra K. , Hitchman Matthew H. , Yoden Shigeo 
Published: March 2023

AbstractThe influence of solar forcing and Galactic Cosmic Rays (GCR) ionization on the global distribution of clouds is investigated using 42 years ERA-5 data (1979–2020). In the mid-latitudes over Eurasia, GCR and cloudiness are negatively correlated, which argues against the ionization theory of enhanced cloud droplet nucleation due to increased GCR during minima in the solar cycle. In the tropics, the solar cycle and cloudiness are positively correlated in regional Walker circulations below 2 km altitude. The phase relationship between amplification of regional tropical circulations and the solar cycle is consistent with total solar forcing, rather than modulation of GCR. However, in the intertropical convergence zone, changes in the cloud distribution are consistent with a positive coupling with GCR in the free atmosphere (2–6 km). This study opens some future challenges and research directions, and clarifies how atmospheric circulation at the regional scale can help in understanding solar-induced climate variability.
Toward low-loss mid-infrared Ga2O3–BaO–GeO2 optical fibers
Authors: Guérineau Théo , Aouji Samar , Morency Steeve , Calzavara Florian , Larochelle Patrick , Labranche Philippe , Lapointe Jerome , Danto Sylvain , Cardinal Thierry , Fargin Evelyne , Bernier Martin , Vallée Réal , Messaddeq Younès 
Published: March 2023

AbstractThe development of efficient and compact photonic systems in support of mid-infrared integrated optics is currently facing several challenges. To date, most mid-infrared glass-based devices are employing fluoride or chalcogenide glasses (FCGs). Although the commercialization of FCGs-based optical devices has rapidly grown during the last decade, their development is rather cumbersome due to either poor crystallization and hygroscopicity resilience or poor mechanical-thermal properties of the FCGs. To overcome these issues, the parallel development of heavy-metal oxide optical fiber from the barium-germanium-gallium oxide vitreous system (BGG) has revealed a promising alternative. However, over 30 years of fiber fabrication optimization, the final missing step of drawing BGG fibers with acceptable losses for meters-long active and passive optical devices had not yet been reached. In this article, we first identify the three most important factors that prevent the fabrication of low-loss BGG fibers i.e., surface quality, volumic striae and glass thermal-darkening. Each of the three factors is then addressed in setting up a protocol enabling the fabrication of low-loss optical fibers from gallium-rich BGG glass compositions. Accordingly, to the best of our knowledge, we report the lowest losses ever measured in a BGG glass fiber i.e., down to 200 dB km−1 at 1350 nm.