bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Updated View of the Water Masses on the Northeast Greenland Shelf and Their Link to the Laptev Sea and Lena River
Authors: Willcox E. W. , Bendtsen J. , Mortensen J. , Mohn C. , Lemes M. , Juul‐Pedersen T. , Holding J. , Møller E. F. , Sejr M. K. , Seidenkrantz M.‐S. , Rysgaard S. 
Published: April 2023

AbstractThe Northeast Greenland shelf is a broad Arctic shelf located between Greenland and Fram Strait. It is the principal gateway for sea ice export and sea ice‐associated freshwater from the Arctic Ocean. Sea ice thickness has decreased by 15% per decade since the early 1990s and meteoric freshwater discharge has increased. The consequence of changing sea‐ice and freshwater conditions in the region on ocean dynamics and the biological system remains unknown. Determining the source(s) of freshwater is important to be able to understand how the area will react to future upstream change. Here we present a synoptic survey of the Northeast Greenland shelf and slope with observations of hydrography, the nutrients nitrate, phosphate and silicate, and conservative tracers δ18O, δ2H, and total alkalinity during late summer 2017. We compare these to previously published values, including those which identify Pacific and Atlantic water, the Siberian shelf seas, and the six largest Arctic rivers. We show that a major source of freshwater on the Northeast Greenland shelf during late summer 2017 is the Laptev Sea and find no conclusive evidence of Pacific Water. Our observations indicate a direct link between Northeast Greenland hydrology and processes occurring on Eurasian shelves.
Ageostrophic Contribution by the Wind and Waves Induced Flow to the Lateral Stirring in the Mediterranean Sea
Authors: Morales‐Márquez Verónica , Hernández‐Carrasco Ismael , Fox‐Kemper Baylor , Orfila Alejandro 
Published: April 2023

AbstractWe study the impact of the Ekman currents and Stokes drift on the surface horizontal transport and mixing properties of the Mediterranean Sea. Finite Size Lyapunov Exponents (FSLE) at the ocean surface are computed over the whole basin using 25 years of satellite altimetry derived geostrophic currents, 10‐m wind velocity and wave fields. We find that the transport pathways unveiled by the geostrophic Lagrangian Coherent Structures (LCS) are significantly modified by the ageostrophic currents (i.e., Ekman and Stokes induced velocities), often leading to a decrease of the retention capacity of the eddies. An exhaustive assessment of the regional and temporal variability of the FSLE shows an increase of the horizontal mixing processes, due to the ageostrophic component, up to 37% in regions such as the Gulf of Lion or the Aegean Sea, during the seasons where wind and waves are intense and persistent. Positive trends in the total FSLE (up to 1.2% of the value of FSLE per year in some regions) suggest that Mediterranean Sea has experienced a significant increase in the intensity of the mixing processes over the last decades. Ageostrophic features are considered to play a role in determining the properties of the relative dispersion. Through the analysis of the Lagrangian Anisotropy Index (LAI) using virtual and real pair of drifters, we observe that the particle dispersion is mainly dominated by the zonal flow, and that the ageostrophic currents induce meridional dispersion, particularly in regions where wind and wave are intensified.
Time‐Lapse Seafloor Surveys Reveal How Turbidity Currents and Internal Tides in Monterey Canyon Interact With the Seabed at Centimeter‐Scale
Authors: Wolfson‐Schwehr M. , Paull C. K. , Caress D. W. , Gwiazda R. , Nieminski N. M. , Talling P. J. , Carvajal C. , Simmons S. , Troni G. 
Published: April 2023

AbstractHere we show how ultra‐high resolution seabed mapping using new technology can help to understand processes that sculpt submarine canyons. Time‐lapse seafloor surveys were conducted in the axis of Monterey Canyon, ∼50 km from the canyon head (∼1,840 m water depth) over an 18‐month period. These surveys comprised 5‐cm resolution multibeam bathymetry, 1‐cm resolution lidar bathymetry, and 2‐mm resolution stereophotographic imagery. Bathymetry data reveal centimeter‐scale textures that would be undetectable by more traditional survey methods. Upward‐looking Acoustic Doppler Current Profilers at the site recorded the flow character of internal tides and the passage of three turbidity currents, while sediment cores collected from the site record flow deposits. Combined with flow and core data, the bathymetry shows how turbidity currents and internal tides modify the seabed. The turbidity currents drape sediment across the site, infilling bedform troughs and smoothing erosional features carved by the internal tides (e.g., rippled scours). Turbidity currents with speeds of 0.9–3.3 m/s failed to cause notable bedform movement, which is surprising given that flows with similar speeds produced rapid bedform migration elsewhere, including the upper Monterey Canyon. The lack of migration may be related to the character of the underlying substrate or indicate that turbidity currents at the site lack dense, near‐bed layers. The scale of scours produced by the internal tides (≤0.7 m/s) approaches the scale of features recorded in the ancient rock record. Thus, these results illustrate how the scale gap between seabed mapping technology and the rock record may eventually be bridged.
Response of Two Glaciers in Different Climate Settings of the Tibetan Plateau to Climate Change Through Year 2100 Using a Hybrid Modeling Approach
Authors: Han Pengfei , Long Di , Zhao Fanyu , Slater Louise J. 
Published: April 2023

AbstractGlaciers in High Mountain Asia (HMA) have been continuously retreating over the past four decades under global warming, affecting water availability for humans and ecosystems. However, the potential differences in mass loss and glacier runoff between maritime and continental glaciers of HMA are poorly understood. To understand the response of both types of glaciers to climate change, this study developed a hybrid modeling approach suited to data‐sparse regions, and applied the proposed approach to the Parlung No. 94 Glacier (a maritime glacier) and the Dongkemadi Glacier (a continental glacier) to project glacier change under climate change. Results show that the maritime glacier has a higher balance amplitude and more negative net balance than the continental glacier. Forced by model outputs from the sixth phase of the Coupled Model Intercomparison Project, both glaciers are projected to melt almost entirely by year 2100 under the highest emission scenario (SSP585). The projected year by which the continental glacier's volume (area) is expected to halve, relative to year 2018, is about 5–11 (14) years later than that of the maritime glacier due to lower glacier retreat rates. Glacier runoff projections indicate that the peak glacier runoff occurs later and is larger for higher emission scenarios and for the continental glacier than the maritime glacier. The peak water year for the Dongkemadi Glacier is projected to occur about 24–35 years later than for the Parlung No. 94 Glacier, for which it is expected to occur by the mid‐21st‐century.
Paired Air and Stream Temperature Analysis (PASTA) to Evaluate Groundwater Influence on Streams
Authors: Hare Danielle K. , Benz Susanne A. , Kurylyk Barret L. , Johnson Zachary C. , Terry Neil C. , Helton Ashley M. 
Published: April 2023

AbstractGroundwater is critical for maintaining stream baseflow and thermal stability; however, the influence of groundwater on streamflow has been difficult to evaluate at broad spatial scales. Techniques such as baseflow separation necessitate streamflow records and do not directly indicate whether groundwater inflow may be sourced from more dynamic shallow flowpaths. We present a web tool application PASTA (Paired Air and Stream Temperature Analysis; https://cuahsi.shinyapps.io/pasta/) that capitalizes on increased public stream temperature data availability and large‐scale, gridded climate observations to provide new and efficient insights regarding relative groundwater influence on streams. PASTA analyzes paired air and stream water temperature signals to evaluate spatiotemporal patterns in stream thermal sensitivity and relative groundwater influence, including inference regarding the dominant source groundwater depth (shallow or deep (i.e., approximately >6 m depth)). The tool is linked to publicly available stream temperature datasets and accepts user‐uploaded datasets. As local air temperature is not often monitored, PASTA pulls daily air temperature data from the comprehensive Daymet products when directly measured data are unavailable, allowing the repurposing of existing stream temperature data. After data are selected or uploaded, the tool (a) fits sinusoidal curves of daily stream and air temperatures by year (water or calendar) to indicate groundwater influence characteristics and (b) performs linear regressions for stream versus air temperatures to indicate stream thermal sensitivity. Results are exported in ASCII file format, creating an efficient and approachable analysis tool for the adoption of newly developed heat tracing analysis from stream reach to landscape scales.
Implementing an Integrated Psychology, Social Services, and Pediatric Diabetes Clinic
Authors: Totka Joan Pennington , Smith Jacquelyn , Brown Sandra , Singh Maharaj , Parton Elaine , Cvecko Dawn , Fortin Heather , Wolfgram Peter M. 
Published: April 2023

Objective: Integrated behavioral health services are rarely implemented as a shared space practice in pediatric diabetes specialty clinics. This paper outlines the clinical outcomes, lessons learned, facilitators, and barriers for supporting youth with diabetes and behavioral health issues whose hemoglobin A1c (A1c) was higher than the target range and their families. Methods: Using the Iowa Model of implementation, we redesigned the “Regular Engagement And Connection for Health” (REACH) program to integrate health psychology and social services in a shared space clinic for monthly or bimonthly diabetes visits (REACH+). This clinic focused on partnering with families, screening for behavioral and social determinants of health (SDOH), and identifying goals to provide holistic care. This paper presents program evaluation data from the first 50 youth–parent dyads. Results: A team in which all roles were respected and valued was critical to successfully implementing REACH+. Youth in the REACH+ clinic decreased their A1c, emergency room visits, and hospitalizations. Forty percent of youth returned to the standard diabetes clinic. Parents reported fewer resource needs associated with SDOH. Conclusions: A structured implementation process for the planning, delivery, and evaluation of a fully integrated shared space pediatric diabetes clinic promoted team cohesion, consensus, and ongoing administrative support along with positive patient and family outcomes. Implications for Impact Statement Using an implementation process to build a shared space healthcare team, including psychology and social services, was key to supporting patient and family health outcomes for youth with diabetes. Outcomes included improved A1c, and fewer emergency health and resource needs. Forty percent of youth returned to standard diabetes care.
A Randomized Pilot Trial of a Text Messaging Intervention for Sleep Improvement and Weight Control in Emerging Adults
Authors: Jensen Chad D. , Duraccio Kara M. , Barnett Kimberly A. , Zaugg Kelsey K. , Fortuna Christopher , Giraud-Carrier Christophe , Woolford Susan J. 
Published: April 2023

Objective: Text messaging is a promising weight loss intervention modality for emerging adults who are overweight, but few studies exist to guide intervention development. Furthermore, sleep is emerging as a salient intervention target for weight management. This study examines feasibility and acceptability of two text messaging interventions for weight control in emerging adults, one of which includes sleep intervention content. Methods: Forty-three emerging adults who were overweight (BMI ≥ 25 < 30; ages 18–21) were randomized to receive either a text messaging intervention focused on diet and physical activity change, or a text messaging intervention designed to modify sleep behavior plus diet and physical activity. The interventions included motivational interviewing, education around health behaviors, physical activity, and dietary goal setting, as well as sleep goals for those in the sleep condition. All participants self-monitored diet, physical activity, and weight via text message for 3 months; those in the sleep intervention monitored nightly sleep duration. Results: Intervention acceptability and engagement were high for both conditions. In exploratory analyses, we observed that body mass index remained stable across assessments, regardless of condition. Participants in the sleep modification condition showed significantly greater sleep duration from pre- to post-intervention than those in the standard intervention. Conclusions: This study demonstrates that a weight loss intervention delivered by text messaging, including components designed to improve sleep, is acceptable and feasible for emerging adults who are overweight, and that extended sleep duration may be a benefit of the sleep modification intervention. Implications for Impact Statement This study provides support for the feasibility and acceptability of a text messaging intervention combining traditional behavioral weight loss strategies with sleep intervention in emerging adults. Results also suggest that a weight loss intervention targeting sleep improvements may increase sleep duration.
The Tip60/Ep400 chromatin remodeling complex impacts basic cellular functions in cranial neural crest-derived tissue during early orofacial development
Authors: Gehlen-Breitbach Sebastian , Schmid Theresa , Fröb Franziska , Rodrian Gabriele , Weider Matthias , Wegner Michael , Gölz Lina 
Published: April 2023

AbstractThe cranial neural crest plays a fundamental role in orofacial development and morphogenesis. Accordingly, mutations with impact on the cranial neural crest and its development lead to orofacial malformations such as cleft lip and palate. As a pluripotent and dynamic cell population, the cranial neural crest undergoes vast transcriptional and epigenomic alterations throughout the formation of facial structures pointing to an essential role of factors regulating chromatin state or transcription levels. Using CRISPR/Cas9-guided genome editing and conditional mutagenesis in the mouse, we here show that inactivation of Kat5 or Ep400 as the two essential enzymatic subunits of the Tip60/Ep400 chromatin remodeling complex severely affects carbohydrate and amino acid metabolism in cranial neural crest cells. The resulting decrease in protein synthesis, proliferation and survival leads to a drastic reduction of cranial neural crest cells early in fetal development and a loss of most facial structures in the absence of either protein. Following heterozygous loss of Kat5 in neural crest cells palatogenesis was impaired. These findings point to a decisive role of the Tip60/Ep400 chromatin remodeling complex in facial morphogenesis and lead us to conclude that the orofacial clefting observed in patients with heterozygous KAT5 missense mutations is at least in part due to disturbances in the cranial neural crest.
In vivo kinetics of early, non-random methylome and transcriptome changes induced by DNA-hypomethylating treatment in primary AML blasts
Authors: Greve Gabriele , Andrieux Geoffroy , Schlosser Pascal , Blagitko-Dorfs Nadja , Rehman Usama-Ur , Ma Tobias , Pfeifer Dietmar , Heil Gerhard , Neubauer Andreas , Krauter Jürgen , Heuser Michael , Salih Helmut R. , Döhner Konstanze , Döhner Hartmut , Hackanson Björn , Boerries Melanie , Lübbert Michael 
Published: April 2023

AbstractDespite routine use of DNA-hypomethylating agents (HMAs) in AML/MDS therapy, their mechanisms of action are not yet unraveled. Pleiotropic effects of HMAs include global methylome and transcriptome changes. We asked whether in blasts and T-cells from AML patients HMA-induced in vivo demethylation and remethylation occur randomly or non-randomly, and whether gene demethylation is associated with gene induction. Peripheral blood AML blasts from patients receiving decitabine (20 mg/m2 day 1–5) were serially isolated for methylome analyses (days 0, 8 and 15, n = 28) and methylome-plus-transcriptome analyses (days 0 and 8, n = 23), respectively. T-cells were isolated for methylome analyses (days 0 and 8; n = 16). We noted massive, non-random demethylation at day 8, which was variable between patients. In contrast, T-cells disclosed a thousand-fold lesser, random demethylation, indicating selectivity of the demethylation for the malignant blasts. The integrative analysis of DNA demethylation and transcript induction revealed 87 genes displaying a significant inverse correlation, e.g. the tumor suppressor gene IFI27, whose derepression was validated in two AML cell lines. These results support HMA-induced, non-random early in vivo demethylation events in AML blasts associated with gene induction. Larger patient cohorts are needed to determine whether a demethylation signature may be predictive for response to this treatment.
Genome-scale CRISPR screen reveals neddylation to contribute to cisplatin resistance of testicular germ cell tumours
Authors: Funke Kai , Einsfelder Ulf , Hansen Aylin , Arévalo Lena , Schneider Simon , Nettersheim Daniel , Stein Valentin , Schorle Hubert 
Published: April 2023

AbstractBackgroundType II testicular germ cell tumours (TGCT) are the most prevalent tumours in young men. Patients suffering from cisplatin-resistant TGCTs are facing very poor prognosis demanding novel therapeutic options. Neddylation is a known posttranslational modification mediating many important biological processes, including tumorigenesis. Overactivation of the neddylation pathway promotes carcinogenesis and tumour progression in various entities by inducing proteasomal degradation of tumour suppressors (e.g., p21, p27).MethodsWe used a genome-scale CRISPR/Cas9 activation screen to identify cisplatin resistance factors. TGCT cell lines were treated with the neddylation inhibitor (MLN4924)/cisplatin/combination and investigated for changes in viability (XTT assay), apoptosis/cell cycle (flow cytometry) as well as in the transcriptome (3’mRNA sequencing).ResultsNAE1overexpression was detected in cisplatin-resistant colonies from the CRISPR screen. Inhibition of neddylation using MLN4924 increased cisplatin cytotoxicity in TGCT cell lines and sensitised cisplatin-resistant cells towards cisplatin. Apoptosis, G2/M-phase cell cycle arrest, γH2A.X/P27 accumulation and mesoderm/endoderm differentiation were observed in TGCT cells, while fibroblast cells were unaffected.ConclusionsWe identified overactivation of neddylation as a factor for cisplatin resistance in TGCTs and highlighted the additive effect of NAE1 inhibition by MLN4924 in combination with cisplatin as a novel treatment option for TGCTs.
CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3
Authors: You A-bin , Yang Hu , Lai Chun-ping , Lei Wen , Yang Lu , Lin Jia-lin , Liu Shun-cui , Ding Nan , Ye Feng 
Published: April 2023

AbstractCMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95), is elevated by viral infection in a variety of cells. However, the functions of CMTR1 in colorectal cancer (CRC), especially its roles in tumorigenesis and immune regulation, remain unclear. Here, we first identified CMTR1 as a novel oncogene in colorectal cancer. Based on The Cancer Genome Atlas (TCGA) database exploration and human tissue microarray (TMA) analysis, we found that CMTR1 expression was markedly higher in CRC tissues than in adjacent normal tissues. High CMTR1 expression was correlated with poor prognosis in CRC patients. Knockdown (KD) of CMTR1 significantly suppressed cell proliferation and tumorigenicity both in vitro and in vivo, whereas overexpression of CMTR1 resulted in the opposite effects. KEGG pathway analysis revealed differential enrichment in the JAK/STAT signaling pathway in colorectal cancer cells with CMTR1 KD. Mechanistically, suppression of CMTR1 expression inhibited RNAPII recruitment to the transcription start site (TSS) of STAT3 and suppressed STAT3 expression and activation. Furthermore, the efficacy of PD1 blockade immunotherapy was prominently enhanced in the presence of CMTR1 KD via increased infiltration of CD8 + T cells into the tumor microenvironment. Overall, it appears that CMTR1 plays a key role in regulating tumor cell proliferation and antitumor immunity.
Melatonin inhibits bladder tumorigenesis by suppressing PPARγ/ENO1-mediated glycolysis
Authors: Shen Dexin , Deng Zhao , Liu Wei , Zhou Fenfang , Fang Yayun , Shan Danni , Wang Gang , Qian Kaiyu , Yu Mengxue , Zhang Yi , Ju Lingao , Xiao Yu , Wang Xinghuan 
Published: April 2023

AbstractMelatonin is a well-known natural hormone, which shows a potential anticancer effect in many human cancers. Bladder cancer (BLCA) is one of the most malignant human cancers in the world. Chemoresistance is an increasingly prominent phenomenon that presents an obstacle to the clinical treatment of BLCA. There is an urgent need to investigate novel drugs to improve the current clinical status. In our study, we comprehensively explored the inhibitory effect of melatonin on BLCA and found that it could suppress glycolysis process. Moreover, we discovered that ENO1, a glycolytic enzyme involved in the ninth step of glycolysis, was the downstream effector of melatonin and could be a predictive biomarker of BLCA. We also proved that enhanced glycolysis simulated by adding exogenous pyruvate could induce gemcitabine resistance, and melatonin treatment or silencing ofENO1could intensify the cytotoxic effect of gemcitabine on BLCA cells. Excessive accumulation of reactive oxygen species (ROS) mediated the inhibitory effect of melatonin on BLCA cells. Additionally, we uncovered that PPARγ was a novel upstream regulator of ENO1, which mediated the downregulation of ENO1 caused by melatonin. Our study offers a fresh perspective on the anticancer effect of melatonin and encourages further studies on clinical chemoresistance.