bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen
Authors: Rakha Bushra Allah , Ansari Muhammad Sajjad , Zuha Sumiyyah , Akhter Shamim 
Published: June 2023

AbstractThe Indian red jungle fowl population is decreasing in its natural habitat. Its conservation through semen cryopreservation with sufficient live sperm recovery rate is requisite where ascorbic acid could play significant role to mitigate cryo‐incited injuries. The objective was to elucidate the effect of ascorbic acid on freezability of Indian red jungle fowl sperm. Pooled semen was aliquoted and diluted (1:5) with red fowl extender having ascorbic acid: 0.0 (control), 1.0, 2.0 and 4.0 mM. Diluted samples were cryopreserved and semen quality was assessed at post‐dilution, cooling, equilibration and freeze–thawing stages. Sperm metabolic status, antioxidant potential and lipid peroxidation were studied at post‐dilution and freeze–thawing. Sperm motility did not differ (p > .05) in experimental extenders and control at post‐dilution and cooling; however, it was recorded higher (p < .05) with ascorbic acid at 2.0 mM compared with other levels at post‐equilibration and post‐thawing stage. Sperm viability, plasma membrane and acrosome intactness were recorded higher (p < .05) with 2.0 mM ascorbic acid compared with other concentrations of ascorbic acid at all stages of cryopreservation. Sperm metabolic status and antioxidant potential were recorded higher (p < .05), while lipid peroxidation was recorded lowest (p < .05) with 2.0 mM ascorbic acid compared with 1.0, 4.0 mM and control. In conclusion, ascorbic acid at 2.0 mM in red fowl extender improves quality, metabolic status and antioxidant potential of frozen Indian red jungle fowl semen through ameliorating lipid peroxidation.
Viable lives: Life beyond survival in rural North India
Authors: Dyson Jane , Jeffrey Craig 
Published: June 2023

AbstractMany minoritised and marginalised populations, including young people, are debating what constitutes a ‘survivable life’ and, in turn, how life can be arranged so that it is more than just survival. Notwithstanding these trends, however, there is little scholarly work on local discourses and practices of life and viability. This paper addresses this gap by examining the spatial and temporal process through which young people imagine and build viable lives in an area of the Indian Himalayas. We highlight the importance for these young people of building life as ‘jeevan’, an idea particularly associated with survival. We also highlight the significance they attach to protecting a wider, ‘puri life’ (‘whole life’), a process that affords opportunities for enjoyment and ethical fulfilment beyond survival. This account of life thought ‘on the ground’ provides a basis for reflecting on Agamben's (Homo sacer: Sovereign power and bare life. Palo Alto, CA: Stanford University Press; 1998) arguments about the prevalence of ‘bare life’ in the contemporary world.
Extended sample storage for platelet function testing in healthy dogs
Authors: Dickinson Melanie , Abrams‐Ogg Anthony , Blois Shauna L. , Wood R. Darren , Monteith Gabrielle 
Published: June 2023

AbstractBackgroundPlatelet function testing is important for monitoring the effects of antiplatelet therapy but is not readily used due to time constraints for testing and the need for specialized equipment.ObjectivesThis study evaluated the effects of various storage methods on selected platelet function tests to determine if delayed platelet function testing is feasible in canine blood samples. Our hypotheses were that platelet function would not decline during storage and, thus, no differences in test results would be found over time.MethodsThirteen healthy dogs were studied. Citrated blood samples were tested with a Platelet Function Analyzer‐200 (PFA), which mimics high‐shear conditions, using P2Y and CADP cartridges, after being held at room temperature for 2 h and refrigerated for 24 and 48 h. Plateletworks (PW), which measures aggregation based on platelet counting, was performed on an optical hematology analyzer using 10‐min‐old native samples, citrated samples held at room temperature for 3–4 h and refrigerated for 24 and 48 h, and samples stored in the preservative solution, AGGFix, up to 7 days.ResultsPFA closure times increased with storage, especially with the P2Y cartridge. Median aggregation with fresh PW was 94%, and this was maintained at all time points (range of median values 88%–94%). Most samples showed decreased, yet still robust (>70%), aggregation with longer storage. Spontaneous aggregation in citrate was noted in most dogs. AGGFix stabilized platelet aggregates to allow for delayed testing.ConclusionsDelayed platelet function testing is feasible, but ranges of expected values may differ from tests using fresh samples.
Investigation of Thermal-Flow Behavior and Droplet Dynamics of Mist Sweeping Impinging Jet Cooling
Authors: Wang Ting , Abdelmaksoud Ramy 
Published: June 2023

Abstract
This paper presents a 2-D numerical study to investigate the fluid flow behavior and cooling characteristics caused by injecting tiny water droplets into the sweeping air jet through a fluidic oscillator. An unsteady Reynolds-averaged Navier–Stokes (URANS) simulation accompanied with the k–ω SST turbulence model is used in this study. The movement and evaporation of the mist are simulated by using the discrete phase model (DPM). The study has been conducted for a target wall with a constant heat flux of 3,000 W/m2, jet-to-wall distance of 4D, ReD = 2,500, and a mist/air mass ratio of 5% with a droplet size of 5 microns. A comparison between the cooling performance of steady and sweeping jets is presented for two impingement schemes (i.e., confined and unconfined impingement). The approach of using a slip upper wall boundary condition as an alternative to the unconfined impingement scheme is investigated as well. The results show that adding mist provided a temperature reduction of 5–10% on the target wall in all cases when compared to the air cases. Mist mostly follows the air jet behavior in both steady and sweeping jets in both impingement schemes. The liquid droplet coalescence phenomenon prevails in the sweeping jet case, while it is not as significant in the steady jet case. For the confined impingement, both mist jets provided the similar average temperature reduction. However, the steady mist jet introduced a 58% more target wall shear compared to the sweeping mist jet. For the unconfined impingement, the steady mist jet achieved a better average cooling performance compared to that of the sweeping mist jet. However, the steady mist jet introduced a 72% more target wall shear compared to the sweeping mist jet. Using a slip upper wall boundary condition to reduce the computational time resulted in similar average heat transfer distribution on the target wall to the unconfined case. However, the flow pattern, vortical structures, and droplet dynamics were very different.
“Who Am I to Tell Them How to Coach?”—An Analysis of Coach Developers’ Professional Identity Formation
Authors: Davidson James , Townsend Robert C. 
Published: June 2023

Coach developers play a critical role in the development of coaches. However, coach developers themselves remain under-researched, in particular the socially mediated process of becoming a coach developer is poorly understood. Eleven coach developers working across Regional and National Sports Governing Bodies took part in a series of interviews and in situ observations over the course of 12 months. Engaging a reflexive thematic analysis, this paper focuses on the power relations that contour the transitions into coach developer roles. Specifically, the analysis depicts an identity-making process in which power is exerted through different sites and modalities that structure and regulate coach developers’ practice and roles through shared discourses. In efforts to secure their positions and maintain legitimate identities, coach developers operated within an uncritical selective culture, which saw them wield, maintain, and enhance the power provided to them. Building on existing research, we examine the contested nature of what it means to be a coach developer, specifically showcasing the navigation of inherently unstable professional learning cultures and critiquing coach developer training. This work begins to address calls for the voices of coach developers in their research, while identifying the reproduction of a cultural orthodoxy, and the lack of effective in situ coach developer training. Implications from the research suggest that further support and development are required in the training of coach developers in situ, as well as the need for research to interrogate the language and discourse framing the role.
Initiating and Sustaining a Teacher-Initiated Community of Practice as a Form of Continuing Professional Development: Internal Leaders’ Perspectives
Authors: Ko Bomna , Lee Yun Soo , Wallhead Tristan 
Published: June 2023

Background: Communities of practice (CoP) have been proposed as a promising form of continuing professional development for teachers’ learning. However, there remains limited understanding of the processes and factors that sustain teacher-initiated CoP. Purpose: This study provides a qualitative examination of a group of internal leaders’ perspectives on how a large teacher-initiated CoP in South Korea was established and sustained as an effective form of continuing professional development. Methods: Data included semistructured interviews with the internal leaders, Website artifacts, and researchers’ reflective journals, and were analyzed through inductive analysis. Results: Findings included the following: (a) shared passion for good teaching, (b) widening professional network, (c) collaboration through sharing, and (d) continuing professional development opportunities via technology. Discussion: The CoP were initiated by a group of teachers with a shared value for improving their teaching in schools and were sustained through online and offline interactions that created a safe space for sharing ideas, collaborative interactions, and learning opportunities.
Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial
Authors: Shitara Kohei , Di Bartolomeo Maria , Mandala Mario , Ryu Min-Hee , Caglevic Christian , Olesinski Tomasz , Chung Hyun Cheol , Muro Kei , Goekkurt Eray , McDermott Raymond S , Mansoor Wasat , Wainberg Zev A , Shih Chie-Schin , Kobie Julie , Nebozhyn Michael , Cristescu Razvan , Cao Z Alexander , Loboda Andrey , Özgüroğlu Mustafa 
Published: June 2023

BackgroundIn the randomized, controlled, phase III KEYNOTE-061 trial, second-line pembrolizumab did not significantly prolong overall survival (OS) versus paclitaxel in patients with PD-L1-positive (combined positive score ≥1) advanced gastric/gastroesophageal junction (G/GEJ) cancer but did elicit a longer duration of response and offered a favorable safety profile. This prespecified exploratory analysis was conducted to evaluate associations between tumor gene expression signatures and clinical outcomes in the phase III KEYNOTE-061 trial.MethodsUsing RNA sequencing data obtained from formalin-fixed, paraffin-embedded baseline tumor tissue samples, we evaluated the 18-gene T-cell-inflamed gene expression profile (TcellinfGEP) and 10 non-TcellinfGEP signatures (angiogenesis, glycolysis, granulocytic myeloid-derived suppressor cell (gMDSC), hypoxia, monocytic MDSC (mMDSC), MYC, proliferation, RAS, stroma/epithelial-to-mesenchymal transition/transforming growth factor-β, WNT). The association between each signature on a continuous scale and outcomes was analyzed using logistic (objective response rate (ORR)) and Cox proportional hazards regression (progression-free survival (PFS) and OS). One-sided (pembrolizumab) and two-sided (paclitaxel) p values were calculated for TcellinfGEP (prespecified α=0.05) and the 10 non-TcellinfGEP signatures (multiplicity-adjusted; prespecified α=0.10).ResultsRNA sequencing data were available for 137 patients in each treatment group. TcellinfGEP was positively associated with ORR (p=0.041) and PFS (p=0.026) for pembrolizumab but not paclitaxel (p>0.05). The TcellinfGEP-adjusted mMDSC signature was negatively associated with ORR (p=0.077), PFS (p=0.057), and OS (p=0.033) for pembrolizumab, while the TcellinfGEP-adjusted glycolysis (p=0.018), MYC (p=0.057), and proliferation (p=0.002) signatures were negatively associated with OS for paclitaxel.ConclusionsThis exploratory analysis of tumor TcellinfGEP showed associations with ORR and PFS for pembrolizumab but not for paclitaxel. TcellinfGEP-adjusted mMDSC signature was negatively associated with ORR, PFS, and OS for pembrolizumab but not paclitaxel. These data suggest myeloid-driven suppression may play a role in resistance to PD-1 inhibition in G/GEJ cancer and support a strategy of considering immunotherapy combinations which target this myeloid axis.Trial registration numberNCT02370498.
On the mechanics of filtered compacted consolidated and overconsolidated iron ore tailings at high pressures
Authors: Silva João Paulo de Sousa , Carvalho João Vítor de Azambuja , Wagner Alexia Cindy , Cacciari Pedro Pazzoto , Consoli Nilo Cesar 
Published: June 2023

The mechanics of filtered compacted normally consolidated (NC) and overconsolidated (OC) iron ore tailings (IOTs) was studied by performing triaxial testing at high pressures of up to 120 MPa. The OC specimens were obtained by isotropically compressing compacted IOT to 120 MPa, unloading it to distinct confining pressures, and shearing at constant radial stress. Particle size distribution analyses were used to examine the effect of compression and shear stresses on particle breakage. Additionally, the triaxial test results illustrate the stress history influence on the deviatoric stress–axial strain–volumetric strain curves, as well as on the IOT peak strength. Furthermore, these results also show that the amount of breakage during the shearing stage plays an essential role in the geomaterial’s response, marking the existence of curved critical state locus in both v–ln p′ and q– p′ planes, which is unique in the studied confined stress range (4–120 MPa) for the compacted NC and OC (considering OC ratio up to 30) IOTs.
Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
Authors: Asaro Antonela , Pinoni Silvina A. , Lorin-Nebel Catherine , Ituarte Romina B. 
Published: June 2023

To understand the response of freshwater organisms to rising environmental salinity, it is essential to investigate their osmo-ionic regulatory physiology. Our laboratory experiment investigated the transfer of Palaemon argentinus (Nobili, 1901) from 2‰ (control condition) to concentrated salinity (15 and 25‰) for short- (6 h), medium- (48 h), and long-term (>504 h) acclimation periods. We measured relevant parameters in the shrimp’s haemolymph, the time course of the response of branchial V–H+–ATPase (VHA), Na+, K+–ATPase (NKA), carbonic anhydrase (CA) activity, and muscle water content. Upon prolonged acclimation to 15‰ (hyper-regulating condition), shrimp reached a new steady state of haemolymph osmolality by tightly adjusting ion concentrations to levels higher than the external medium. While NKA and CA activities recovered their pre-transfer levels, the downregulation of VHA suggests other functions rather than ion uptake after prolonged acclimation to 15‰. The activity of the three transport-related enzymes remained almost unchanged at the highest salinity (isosmotic condition), leading to increasing osmotic pressure and ion concentration after prolonged acclimation to 25‰. Although the freshwater shrimp studied here retains a certain degree of tolerance to high salinity, a common trait in palaemonid shrimps, our results highlight that 25‰ represents a significant hypertonic challenge for this species.
Exclusive photo- and electroproduction of excited light vector mesons via holographic model
Authors: Henkels Cheryl , de Oliveira Emmanuel G. , Pasechnik Roman , Trebien Haimon 
Published: June 2023

AbstractIn this paper, we study total and differential observables of electro- and photoproduction of light $$\rho $$
ρ
, $$\omega $$
ω
and $$\phi $$
ϕ
mesons as functions of the center-of-mass energy of the $$\gamma p$$

γ
p

collision and momentum transfer squared |t|. The corresponding vector mesons wave functions have been computed in the framework of relativistic AdS/QCD holographic approach. A satisfactory description of all available data on ground-state $$\rho (1S)$$

ρ
(
1
S
)

, $$\omega (1S)$$

ω
(
1
S
)

and $$\phi (1S)$$

ϕ
(
1
S
)

mesons production cross sections has been achieved in the color dipole picture. Finally, the key observables of excited $$\rho (2S)$$

ρ
(
2
S
)

, $$\omega (2S)$$

ω
(
2
S
)

and $$\phi (2S)$$

ϕ
(
2
S
)

states production in $$\gamma ^{(*)} p$$

γ

(

∗
)

p

collisions have been presented here using a common wave function formalism. This study reveals a large theoretical uncertainty coming from the modeling of the partial dipole amplitude in the nonperturbative kinematical domain. Hence, the latter could benefit from future measurements of photoproduction of the excited states.
Probing supermassive black hole binaries with orbital resonances of laser-ranged satellites
Authors: Du Minghui , Deng Qiong , Bian Yifan , Luo Ziren , Xu Peng 
Published: June 2023

AbstractCoalescing supermassive black hole binaries (SMBHBs) are the primary source candidates for low frequency gravitational wave (GW) detections, which could bring us deep insights into galaxy evolutions over cosmic time and violent processes of spacetime dynamics. Promising candidates had been found based on optical and X-ray observations, which claims for new and ready-to-use GW detection approaches before the operations of space-borne antennas. We show that, satellite laser ranging (SLR) missions could serve as probes of coalescing SMBHBs through the GW-induced resonant effects. Lasting and characteristic imprints caused by such resonances in the residual distances or accelerations from SLR measurements are studied, and the detection SNR is analyzed with both the current and future improved ranging precisions. Within redshift $$z \sim 1$$

z
∼
1

, the threshold SNR = 5 requires 1–2 years of accumulated data for the current precision and months of data for improved precision, which are workable for the data processing of SLR missions. Meanwhile, joint detections with multiple SLR missions could further improve the total SNR and the confidence level. Such a detection scheme could fulfill the requirement of a tentative SMBHB probe during the preparing stage of LISA and Taiji, and it requires no further investment to any new and advanced facilities. It is also worthwhile to look back and re-process the archived data from the past decades, in where resonant signals from SMBHBs might be hidden.
Trackable Intratumor Microdosing and Spatial Profiling Provide Early Insights into Activity of Investigational Agents in the Intact Tumor Microenvironment
Authors: Derry Jonathan M.J. , Burns Connor , Frazier Jason P. , Beirne Emily , Grenley Marc , DuFort Christopher C. , Killingbeck Emily , Leon Michael , Williams Claire , Gregory Mark , Houlton Jeffrey , Clayburgh Daniel , Swiecicki Paul , Huszar Dennis , Berger Allison , Klinghoffer Richard A. 
Published: June 2023

Abstract

Purpose:
Cancer drug development is currently limited by a paradigm of preclinical evaluation that does not adequately recapitulate the complexity of the intact human tumor microenvironment (TME). To overcome this, we combined trackable intratumor microdosing (CIVO) with spatial biology readouts to directly assess drug effects in patient tumors in situ.

Experimental Design:
In a first-of-its-kind phase 0 clinical trial, we explored the effects of an investigational stage SUMOylation-activating enzyme (SAE) inhibitor, subasumstat (TAK-981) in 12 patients with head and neck carcinoma (HNC). Patients scheduled for tumor resection received percutaneous intratumor injections of subasumstat and vehicle control 1 to 4 days before surgery, resulting in spatially localized and graded regions of drug exposure (∼1,000–2,000 μm in diameter). Drug-exposed (n = 214) and unexposed regions (n = 140) were compared by GeoMx Digital Spatial Profiler, with evaluation at single-cell resolution in a subset of these by CosMx Spatial Molecular Imager.

Results:
Localized regions of subasumstat exposure revealed SUMO pathway inhibition, elevation of type I IFN response, and inhibition of cell cycle across all tumor samples. Single-cell analysis by CosMx demonstrated cell-cycle inhibition specific to the tumor epithelium, and IFN pathway induction commensurate with a TME shift from immune-suppressive to immune-permissive.

Conclusions:
Pairing CIVO with spatial profiling enabled detailed investigation of response to subasumstat across a diverse sampling of native and intact TME. We demonstrate that drug mechanism of action can be directly evaluated in a spatially precise manner in the most translationally relevant setting: an in situ human tumor.
The Phonetic and Morphosyntactic Dimensions of Grammatical Gender in Spanish Heritage Language Acquisition
Authors: Pérez-Leroux Ana Teresa , López Yadira Álvarez , Barreto Miguel , Cuza Alejandro , Marinescu Irina , Yang Jierui , Colantoni Laura 
Published: June 2023

Abstract
Previous studies disagree as to whether heritage bilinguals demonstrate loss of knowledge of Spanish grammatical gender. As phonetic variability is known to affect the acquisition of certain grammatical markers, we examine whether bilinguals’ gender difficulties relate to bilingual contact-induced phonetic variability, namely, reduction in the inventory of word-final unstressed vowels. We analyzed narratives from children in the United States (n = 49, ages 4–12). All NP  s (n = 1415) were analyzed for structure, noun class, and morphology. Word-final vowels were sub-selected for acoustic analyses. Morpho-syntactically, group results show high accuracy with gender (95%), but with wide individual variation (44%–100%). Speakers also show individual variability and substantive numbers of vowel misclassifications (6%–33%) with higher variability for /a/ and /o/. We found bilingual effects in both domains but no association between phonetics and gender accuracy. These findings have implications for the relationship between phonetics and grammar, and for the morphosyntax of Spanish gender.