bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of anti-platelet factor4/polyanionic antibodies, thrombocytopenia, and thrombosis after COVID-19 vaccination with ChAdOx1 nCoV-19 in Thais
Authors: Boonyawat Kochawan , Mmt Tichayapa Phojanasenee , Noikongdee Phichchapha , Mmt Pornnapa Police , Chantrathammachart Pichika , Niparuck Pimjai , Puavilai Teeraya , Phuphuakrat Angsana , Angchaisuksiri Pantep 
Published: May 2023

Abstract
Background
The prevalence of anti-platelet factor 4 (PF4)/polyanionic antibodies occurring after vaccination with ChAdOx1 nCoV-19 is low. Most of these antibodies are not associated with vaccine-induced thrombotic thrombocytopenia. It remains unknown whether these antibodies are preexisting or occur as a result of vaccination. In this study, we demonstrated the incidence of anti-PF4/polyanionic antibodies, thrombocytopenia, and thrombosis after vaccination with ChAdOx1 nCoV-19 in a large cohort of Thais.
Methods
We conducted a prospective study in a cohort of health care workers and members of the general population who received COVID-19 vaccination with ChAdOx1 nCoV-19. Blood collection for complete blood count, D-dimer, and anti-PF4/polyanionic antibodies was performed before vaccination (day 0), day 10, and day 28 after vaccination. Anti-PF4/polyanionic antibodies were detected using enzyme-link immunosorbent assay (ELISA). Functional assay was performed for all positive ELISA tests.
Results
A total of 720 participants were included in the study. 214 participants received both the first and second doses, 91 participants received only the first, 51 received only the second, and 364 received the third booster dose of ChAdOx1 nCoV-19. Median age was 42 years (IQR, 34–53). 67% of participants were female. Three participants developed seroconversion, yielding an incidence of vaccination-induced anti-PF4/polyanionic antibodies of 0.42% (95% confidence interval 0.08, 1.23). Fourteen (1.9%) participants had preexisting anti-PF4/polyanionic antibodies before the vaccination but their optical density of anti-PF4/polyanionic antibodies did not significantly increase over time. None of the anti-PF4/polyanionic positive sera induced platelet aggregation. Abnormal D-dimer levels following vaccination were not different among the positive and negative anti-PF4/polyanionic groups (11.8% vs 13.2%, p = 0.86). Thrombocytopenia occurred in one person with negative anti-PF4/polyanionic antibodies. No clinical thrombosis or bleeding occurred.
Conclusion
We found a low incidence of seroconversion of anti-PF4/polyanionic antibodies after vaccination with ChAdOx1 nCoV-19 in Thais. Most of the anti-PF4/polyanionic antibodies were preexisting and did not significantly increase after vaccination with ChAdOx1 nCoV-19. Following vaccination, some participants with anti-PF4/polyanionic antibodies had elevated D-dimer levels, while only one developed thrombocytopenia and no thrombotic events were observed.
Intestinal microbiota is a key target for load swimming to improve anxiety behavior and muscle strength in Shank3-/- rats
Authors: An Shasha , Zhen Zhiping , Wang Shijiao , Sang Mingze , Zhang Shuai 
Published: May 2023

Abstract
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social disorder and stereotypical behavior, and its incidence rate is increasing year by year. Early treatment is considered to be a critical period for the prognosis of young children with ASD. Swimming, due to its comfort, is often used to develop the enthusiasm of young children for activities, and has a good effect in the treatment of ASD, But its effective path to be reported. The intestinal microbiota of ASD patients and animal models has been reported to be different from those of healthy controls, and these changes may affect the brain environment. Therefore, whether the intestinal microbiota is involved in the treatment of ASD by early swimming is our concern. In this study, we conducted 8-day old Shank3 gene knockout rats with early load swimming training for 8 weeks, and conducted behavioral, small intestine morphology, and intestinal content sequencing after training. The results showed that early load swimming significantly reduced the stereotyped and anxious behaviors of Shank3−/− rats, increased their muscle strength, increased the length of intestinal villi and the width of muscular layer after Shank3 knockout, and affected the abundance of intestinal microorganisms, the abundances with statistical significance are Lactobacillus, Lachnospiraceae, Alloprevotella. In order to further confirm the role of intestinal microorganisms in it, we designed a 14 day intestinal stool transplantation experiment. Fecal microbiota transplantation concluded that load swimming can significantly reduce the anxiety behavior of Shank3 rats, increase their muscle strength, change the structure of the small intestine, and affect the abundance of intestinal contents, The abundance of Epsilonbateraeota, Prevotella, and Bacteroides significantly changed after transplantation. Our findings confirm the possibility of early load swimming therapy for ASD and explain that intestinal microbiota is a key pathway for early exercise therapy for ASD.
Caffeic acid, a dietary polyphenol pre-sensitizes PDAC to chemotherapeutic drug
Authors: Gupta Shruti , Tak Harshita , Rathore Khushhal , Banavath Hemanth Naick , Tejavath Kiran Kumar 
Published: May 2023

Abstract
Purpose: Resistance to chemotherapeutics is an eminent cause which leads for search of options that help in diminution of Pancreatic Ductal Adenocarcinoma (PDAC) by overcoming resistance issues. Caffeic acid (CFA), a polyphenol occurring in many dietary foods is known to show anti-diabetic and anticancer properties potential.Methods: To unveil effect of CFA on PDAC we carried out this research in PDAC cells, following which we checked combination effect of CFA and chemotherapeutics and pre-sensitization effects of CFA. Multitudinous web-based approaches were applied for identifying CFA targets in PDAC and then getting their interconnections.Results and conclusions: Subsequently, we manifested CFA affects by in-vitro analysis showing IC50 concentrations of 37.37µM and 15.06µM on Panc-1 and Mia-PaCa-2 respectively. Combination index of CFA with different drugs was explored that gave no significant results leading to further investigate pre-sensitizing effects. CFA pre-sensitization reduced IC50 concentration of doxorubicin in both PDAC cell lines which also triggered ROS generation determined by DCFH-DA assay. Gene expression analysis revealed that CFA acts differently on both cell lines and triggers distinct signalling to overcome resistance. Collectively, this study investigated role of CFA as PDAC therapeutics and explored mechanism in mitigating resistance of PDAC by sensitizing to chemotherapeutics.
The Impact of Remote Work on Employee Productivity and Well-being: A Comparative Study of Pre- and Post-COVID-19 Era
Authors: S Sivaprakash J , M Venkatesh 
Published: May 2023

Abstract
The abrupt change towards remote work brought on by the COVID-19 pandemic has had a tremendous impact on how employees work and their well-being. By contrasting the pre- and post-COVID-19 eras, this study seeks to understand the effects of distant work on worker productivity and wellbeing. The study will be carried out utilising a mixed-methods approach with a sample of employees working remotely in various organisations, including surveys, interviews, and focus groups. The study's first portion will examine the effects of remote work on employee productivity by contrasting pre-COVID-19 and post-COVID-19 levels of productivity. The study will look into the variables like technological accessibility, work-life balance, and degree of supervision that may have contributed to the change in productivity. The effect of remote work on employee well-being will be looked at in more detail in the study's second portion. The study will look into how working remotely affects employees' stress levels, social connections, and mental health. The results of this study will give organisations information about the effects of remote work on worker productivity and wellbeing that they can use to enhance their policies and procedures around remote work. The study will add to the body of knowledge already available on remote work and offer advice to organisations on how to improve its efficacy and foster employee wellbeing. In the end, the study will assist businesses in understanding the advantages and difficulties of remote employment.
New insight of chemical constituents in Persea americana fruit against obesity via data-driven analysis
Authors: Oh Ki-Kwang , Yoon Sang-Jun , Lee Su-Been , Lee Sang Youn , Gupta Haripriya , Ganesan Raja , Sharma Satya Priya , Won Sung-Min , Jeong Jin-Ju , Kim Dong Joon , Suk Ki-Tae 
Published: May 2023

Abstract
Background: Persea americana fruit (PAF) is a favorable nutraceutical resource that comprises diverse unsaturated fatty acids. Unsaturated fatty acids are significant in dietary supplementation, as they relieve metabolic disorders, including obesity (OB). Unquestionably, a plethora of natural plants contain fatty acids found in PAF. This study focused on the anti-OB efficacy of the non-fatty acids in PAF using a network pharmacology approach.
Results: A total of 41 chemical compounds in PAF were identified by NPASS and other reports. All 41 compounds conformed to Lipinski’s rule, and 52 intersecting targets were selected from the SEA and STP databases. Finally, 31 overlapping targets were selected from the 52 intersecting targets and OB-related targets (3028), which were considered as key proteins against OB, including AKT1 as the uppermost key target. On the bubble chart, the PPAR signaling pathway had the highest rich factor and its modulation was determined as the key mechanism, suggesting that this pathway may have an agonistic function in treating OB. The PFA-signaling pathways-targets-compounds (PSTC) network showed that AKT1 had the greatest degree value. The MDA results showed that AKT1-γ-tocopherol, PPARA-fucosterol, PPARD-stigmasterol, PPARG-fucosterol, NR1H3-campesterol, and ILK-α-tocopherol formed the most stable complexes. These five key compounds were nontoxic, and could be developed as new antagonists to alleviate OB.
Conclusions: In summary, this study suggests that the non-fatty acids in PAF might play central roles in OB. Notably, alpha-tocopherol, gamma-tocopherol, fucosterol, stigmasterol, and campesterol might be key players in the activation on PPAR signaling pathway. However, the results of this study should be further investigated to determine the therapeutic value of these compounds.
Research on a novel non-uniform and asymmetric ultrasonic vibration system and Machined surface characteristics
Authors: Zhao Chongyang , Wang Zhenzhou , Wang Xiaobo , Han Jishan , Zhao Bo 
Published: May 2023

Abstract
A new type of non-uniform and asymmetric ultrasonic vibration system is proposed and applied to the turning process. The research results show that the design theory of the non-uniform and non-symmetric ultrasonic vibration system is accurate and reliable. The designed non-uniform and non-symmetric ultrasonic vibration system has excellent vibration performance and stability, and the established simulation model can predict surface microstructure accurately. The vibration mode output by the non-uniform and non-symmetric ultrasonic vibration system is longitudinal-bending vibration, and the actual vibration performance is excellent. Compared with conventional cutting, the addition of longitudinal-bending ultrasonic vibration under the same conditions can give the machined surface a microscopic texture structure, reduce surface roughness and water contact angle, and improve the surface wettability. The orthogonal experiment results show that there is a correlation between the machining parameters, surface micro-structure parameters, surface wettability, and surface roughness.
Optimization of ultrasonic assisted ethanol extraction of polyphenols from Phyllanthus emblica by Response Surface Methodology
Authors: Wu Yali , Zhang Wei , Guo Lida , Zhang Suqing , Liang Shizhong 
Published: May 2023

Abstract
Polyphenols are indispensable as the main antioxidant substances of P. embaldii. This experiment applied ultrasonic assisted the emblic leafflower fruit polyphenol was extracted with ethanol, and using the response surface method to optimize the extraction results improved, to obtain the parameters of the excellent conditions, mainly for the drying temperature of 80 ℃ emblic leafflower fruit pulp, 60% of the alcohol concentration, solid-liquid ratio, ultrasonic extraction time is 24 min and all the sons of required temperature 45 ℃ of ultrasound. Under the experimental conditions, the yield of polyphenols obtained from P. emballiae was 10.40%, which was almost the same as the ideal prediction value of 10.39%. The established regression model has a good fitting degree.
Using Ribonucleoprotein-based CRISPR/Cas9 to edit single nucleotide on human induced pluripotent stem cells to model type 3 long QT syndrome (SCN5A+/-)
Authors: Ge Ning , Liu Min , Li Rui , Allen Nicholas M. , Galvin Joseph , Shen Sanbing , O'Brien Timothy , Prendiville Terence 
Published: May 2023

Abstract
Human induced pluripotent stem cells (hiPSCs) have been widely used in cardiac disease modelling, drug discovery, and regenerative medicine as they can be differentiated into patient-specific cardiomyocytes. Long QT syndrome type 3 (LQT3) is one of the more malignant congenital long QT syndrome (LQTS) variants with an SCN5A gain-of-function effect on the gated sodium channel. Moreover, the predominant pathogenic variants in LQTS genes are single nucleotide substitutions (missense) and small insertion/deletions (INDEL). CRISPR/Cas9 genome editing has been utilised to create isogenic hiPSCs to control for an identical genetic background and to isolate the pathogenicity of a single nucleotide change. In this study, we described an optimized and rapid protocol to introduce a heterozygous LQT3-specific variant into healthy control hiPSCs using ribonucleoprotein (RNP) and single-stranded oligonucleotide (ssODN). Based on this protocol, we successfully screened hiPSCs carrying a heterozygous LQT3 pathogenic variant (SCN5A+/-) with high efficiency (6 out of 69) and confirmed no off-target effect, normal karyotype, high alkaline phosphatase activity, unaffected pluripotency, and in vitro embryonic body formation capacity within 2 weeks. In addition, we also provide protocols to robustly differentiate hiPSCs into cardiomyocytes and evaluate the electrophysiological characteristics using Multi-electrode Array. This protocol is also applicable to introduce and/or correct other disease-specific variants into hiPSCs for future pharmacological screening and gene therapeutic development.
Dural Venous Sinus Stenting Technique for Idiopathic Intracranial Hypertension in Patients with Tortuous Venous Anatomy.
Authors: Gorjian Mehrnoush , Andrada Jason , Sitko Kevin , Sorte Danielle , Taylor Christopher , Eliyas Javed Khader , Carlson Andrew 
Published: May 2023

Abstract
Venous sinus stenting (VSS) for medically refractory idiopathic intracranial hypertension (IIH) is emerging as a safe and effective alternative to shunting. However, stent navigation past the jugular bulb with commonly used carotid stenting systems via femoral access in cases with tortuous venous anatomy can present a challenge, leading to procedural failure. We present a technical refinement using a cervical access and peripheral vascular stent with a more stable 0.035-inch delivery platform as an alternative to the traditional approach to simplify the procedure and overcome the technical difficulties in cases with tortuous venous anatomy. Our institutional database for patients who had IIH and undergone VSS using the peripheral vascular stent between 2013 and 2023 was retrospectively reviewed. Data on 36 patients (33 women, 3 men, mean age 32 years) was collected. VSS was technically successful in all patients (100%) without major complications or thrombosis. There was one case of minor neck cellulitis treated with oral antibiotics. Three patients underwent repeat stenting, and 2 patients had ventriculoperitoneal shunt placement after stenting due to persistent or recurrent symptoms. All patients (100%) had improvement or resolution of papilledema; however, six patients had evidence of optic atrophy and persistent vision loss. Headache was resolved or improved in 91% of patients. In the presence of tortuous venous anatomy, VSS using cervical access and a peripheral vascular stent with a more stable 0.035-inch delivery platform can be considered as a safe and effective alternative approach with shorter procedure time. This approach is particularly advantageous in situations where the procedure is prolonged or high dose of contrast has been administered due to the technical challenges associated with the traditional use of carotid systems via femoral access for stent delivery.
Collision tumor of primary malignant lymphoma and adenocarcinoma in the colon, diagnosed by molecular genetics analyses: case report and review of literature
Authors: Jiang Meng , Zhou Shengning , Lai Yiming 
Published: May 2023

Abstract
Purpose We report one collision tumor of the ascending colon adenocarcinoma and primary diffuse large B-cell lymphoma, not otherwise specified (DLBCL, NOS) with molecular genetics analyses.
Methods The present case and twenty-two previous cases with collision tumors of primary colorectal lymphoma and adenocarcinoma were reviewed. Clinicopathological characteristics, molecular genetics analyses, possible pathogenesis, management and prognosis of the tumor were analyzed.
ResultsA 74-year-old female patient was found to have an invasive adenocarcinoma in the ascending colon, and underwent laparoscopic radical resection of right colon cancer. The mass comprised of two tumors was found incidentally through postoperative pathological sampling. Molecular genetics and immunohistochemical analyses showed collision tumor of DLBCL, NOS with germinal-center B-cell (GCB) subtype and TP53 mutation, and adenocarcinoma arising in a tubulovillous adenoma in the colon, with BRAF mutation and MLH1 promoter methylation. The mean age of the 23 patients was 73 years. The most cases of the available histopathological staging of adenocarcinoma were stage I (7/16), whereas those of lymphoma components were stage Ⅳ (10/20). The most common histological subtypes of the lymphoma were B-cell lymphomas (22/23). There were 15 cases with follow-up data including 11 alive and 4 dead with a 3-year overall survival rate (OS) of 71.5%.
Conclusions Our report highlights the need for pathologists, radiologists, surgeons and oncologists to be aware of the rare possibility of collision tumors. The accurate molecular genetics analyses and comprehensive treatment including surgery combined with chemotherapy are required for the rare cases.
Risk Factors for Early Recurrence in Patients with Pancreatic Ductal Adenocarcinoma who Underwent Curative Resection
Authors: Murakawa Masaaki , Kawahara Shinnosuke , Takahashi Daishi , Kamioka Yuto , Yamamoto Naoto , Kobayashi Satoshi , Ueno Makoto , Morimoto Manabu , Sawazaki Sho , Tamagawa Hiroshi , Ohshima Takashi , Yukawa Norio , Rino Yasushi , Morinaga Soichiro 
Published: May 2023

Abstract
Background The present study evaluated the clinical characteristics and risk factors for early recurrence in pancreatic ductal adenocarcinoma (PDAC) patients who underwent curative resection, regardless of the use of neoadjuvant chemotherapy, to identify predictive factors associated with early recurrence and poor outcomes as well as to determine the optimal treatment strategy for patients at high risk of early recurrence after surgical resection.Methods Patients who underwent pancreatic resection for PDAC at our institution from 2013 to 2021 were included in this study. We investigated the clinicopathological features of patients in groups: those with recurrence within 6 months, recurrence between 6 and 12 months, and recurrence beyond 12 months or no recurrence. A logistic regression analysis identified covariates associated with early recurrence at 6 and 12 months.Results The study included 403 patients with a median follow-up of 25.7 months. Recurrence was observed in 279 patients, with 14.6% recurring within 6 months, 23.3% within 6–12 months, and 62% after 12 months or not at all. The preoperative CA19-9 level, modified Glasgow prognostic score (mGPS), and positive peritoneal cytology were significant risk factors for early recurrence within 6 months, while positive peritoneal cytology, lymph node metastasis, and the absence of adjuvant chemotherapy were significant risk factors for recurrence within 12 months. For patients who received preoperative chemotherapy or chemoradiotherapy, the preoperative CA19-9 level, mGPS, and positive peritoneal cytology were significant independent risk factors for early recurrence within 6 months, while positive peritoneal cytology, lymph node metastasis, and absence of adjuvant chemotherapy were significant independent risk factors for recurrence within 12 months. The study concluded that the overall survival after surgical resection for potentially resectable PDAC worsened according to the number of risk factors present in the patient.Conclusions We clarified that preoperative CA19-9, positive peritoneal cytology, and the lack of adjuvant chemotherapy were consistent predictors for early recurrence within 6 and 12 months. In addition, an increased number of risk factors affecting the patient was associated with a poorer overall survival after potentially curable resection. Calculating the number of risk factors for early recurrence may be an essential predictive factor when considering treatment strategies.
Caspase-3 specific inhibitors improve lung function in dogs with lung hypoxia reperfusion injury by regulating the Caspase-3/GSDME signaling pathway
Authors: Chen Song , Dou Xuejiao , Guo Yuhan , Pan Jiamei , Luo Junli 
Published: May 2023

Abstract

ObjectiveTo investigate the effect of caspase-3-specific inhibitors of extracorporeal circulation (CPB) on pulmonary ischaemia-reperfusion injury in dogs.MethodsEighteen healthy mongrel dogs were divided into three groups (n = 6) by random number table method, sham-operated group (group C), ischemia-reperfusion group (group I/R) and caspase-3 specific inhibitor (group Z), and the rest of the groups were established as CPB left lung ischemia-reperfusion injury model, and group Z was given caspase-3 specific inhibitor (Z-DEVD) via femoral vein immediately after opening the left pulmonary artery. The caspase-3 specific inhibitor (Z-DEVD-fmk) 0.6mg/Kg was administered to the Z group immediately after opening the left pulmonary artery via the femoral vein. Arterial blood was collected from the femoral artery before chest opening (T1), at the time of shutdown (T2) and at the end of the experiment (T3), and arterial blood gas analysis was performed to calculate respiratory index (RI) and oxygenation index (OI). -N expression, TUNEL assay for apoptosis and HE assay for pathological changes in lung tissue at each time point.ResultsThe expression of RI, Cleaved-caspase3, NLRP3, Clleaved-caspase-1, GSDME-N and apoptotic index in lung tissue increased in the I/R group at T2 and T3 compared with the C group, while the OI decreased (P < 0.05); compared with the I/R group at T3, the expression of RI, Cleaved-caspase3 and apoptotic index in the Z group increased. caspase3, NLRP3, Clleaved-caspase-1, GSDME-N expression and apoptotic index decreased and OI increased in the Z group compared with the I/R group at T3 (P < 0.05); no significant apoptotic cells were seen in the C group at T3; apoptotic cells increased in the I/R group after open left pulmonary arteriovenous reperfusion stimulation, while the Z group had fewer positive cells than the I/R group.ConclusionCaspase-3-specific inhibitors can improve lung function and reduce apoptosis in lung tissue after CPB, it also reduce oxidative stress level and inflammation level. These effects may be achieved by regulating Caspase-3/GSDME signaling pathway and inhibiting pyroptosis.