bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and validation of a novel anoikis-related signature to predict clinical outcomes, TME and treatment response of breast cancer patients
Authors: Liu Qian , Qu Fei , Wu Xuefang , Lu Rongrong , Huang Xiang , Li Wei , Yin Yongmei 
Published: April 2023

Abstract
Background
Breast cancer is the most prevalent malignant among female population worldwide. Anoikis is a key progress during genesis and metastasis of malignant cells. Few studies investigate connections between anoikis and prognosis in breast cancer patients.
Methods
Anoikis-related genes (ARGs) were achieved from GeneCards and Harmonizome portals database. Based on expression patterns of prognostic ARGs, patients were classified as two subtypes and an ARG risk signature was constructed. Based on the formulation, risk score of every individual was calculated. Then, the ability of prognosis prediction was examined by ROC curve and Nomogram. Finally, we analyzed the correlation between TME, signal pathways enriched and treatment response between different risk groups.
Results
Patients were classified into two clusters based on ARG expression. Cluster B was featured by a longer OS. According to the expression profile of prognostic ARGs between clusters, we constructed a risk scoring signature based on five genes. Patients were again divided into the high- and low-risk group according to the score. The high-risk group was characterized by poorer diagnosis, fewer activated immune cells infiltration and worse treatment response to immune checkpoint inhibitors. Finally, the drug sensitivity analysis revealed the potential benefit of the model in supporting clinical decision.
Conclusion
We successfully established an ARG risk scoring system associating expression profile of ARGs with clinicopathological features to make breast cancer management more individualized and rationalized.
A large bande frequencies AC/DC SSHIC Electrical Interface for Piezoelectric Sensors and Energy Harvesting
Authors: Youssef EL HMAMSY , Erroumayssae SABANI , Aymen Mohamed , Chouaib ENNAWAOUI , Mehdi LAADISSI EL , Mehdi LOUALID El , Abdelhadi BOUKNDAL , Ahmed CHEBAK , Abdelowahed HAJJAJI 
Published: April 2023

Abstract

The harvesting energy from the vibration of piezoelectric beam or membrane producing an alternating voltage, requires a passive AC/DC converter to avoid energy losses, and to adapt the electrical signal to the input parameters (voltage, current). The objective of this work is to demonstrate a complete AC/DC converter autonomous system which is powered by a piezoelectric energy harvester. Accordingly, the proposed rectifier has several advantages in terms of efficiency, compatibility, circuit simplicity, and integration into various systems. The AC-DC converter for piezoelectric energy harvesting applications presented in this paper is an improved version of the Synchronized Switch Harvesting on Inductor Corrected (SSHIC) Converter. This modified version provides better results of up to 56% for technology parallel SSHIC-p and 51% for technology series SSHIC-s compared to input power. In addition, it is better in terms of efficiency more than 220% compared to the bridge rectifier with relatively stable output despite varying conditions. In addition, the structures vibrating in sinusoidal or pseudo sinusoidal mode, thus being able to be modeled by a second order. This model is well suited to broadband stresses of multimodal structures.
Investigation of anti-breast cancer potential of a Coral-derived heterocyclic molecule Capillobenzofuranol by inhibiting Estrogen Receptor-α
Authors: Mukerjee Nobendu , Maitra Swastika , Ghosh Arabinda , Akash Shopnil , Panda Siva Prasad , Dey Abhijit , Jha Saurabh Kumar 
Published: April 2023

Abstract
Estrogen receptor α (ER-α) plays a crucial role in the start and progression of breast cancer. ER-α stimulates the expression of oncogenic proteins including Cyclin D1 and c-Myc while inhibiting the expression of cell cycle inhibitors like P21. ER-α has critical functions in the development, survival, and architecture of cancer cells and the regulation of gene expression in these cells. Estrogen receptors are also linked to ER-mediated breast cancer and its progression. In this study, we focused on blocking the active binding site of estradiol (E1), which binds to the ER-α and is known to cause breast cancer. We conducted a virtual screening for approximately 50 natural chemicals that were shown to be overexpressed in ER-α. A target-based approach for drug design was used in this study, which included high throughput screening using molecular docking via AutoDock vina, based on the best-docked phytochemical nutraceuticals, and further investigation employed into the stability and efficacy of the ER-α during 100 ns molecular dynamics and simulation. The results of the post-simulation analysis and binding energy calculation in MMGBSA demonstrated that the nutraceuticals possessed a superior potential for ER inhibition in a concentration dependent manner. Due to its strong affinity for the Estrogen Receptor-protein, it could be an active site inhibitor for ER-mediated breast cancer.
TCPPA-Three factor conditional privacy preserving authentication protocol for smart ambulance in the vehicular Adhoc environment
Authors: Agrahari Abhay Kumar , Venkatesan S. , Varma Late Shirshu 
Published: April 2023

Abstract
The vehicles are connected through the internet in the vehicular Adhoc network environment. When any emergency vehicle like an ambulance arrives, we have to create a green corridor to that ambulance so that it will reach safely. The communication between the ambulance and the other entity like RSU or other vehicles through the open channel, These open channels are the main target by the adversary to get the messages which will transfer between two entities. The adversary will modify, delete or eavesdrop on the message to do the attacks like privileged-insider attack, replay attack, man in the middle attack or other related attacks. In this paper, we will propose the new TCPPA authentication protocol to secure the intelligent transportation system in a vehicular network. We also analyzed the security features in informal security analysis. A formal security analysis is done using the BAN logic. Additionally, we employ the ROR model for mathematical proof and the AVISPA simulation tool for security analysis. At last, an exhaustive comparative analysis will be conducted between the proposed work with some other existing works.
Subtyping treatment response of tirofiban in acute ischemic stroke based on neuroimaging features
Authors: Han Bin , Man Xu , Ding Jian , Tian Xintao , Zhu Xuelian , Yu Jiang , Sun Jinping 
Published: April 2023

Abstract
Our previous trial demonstrated that tirofiban was effective and safe in acute ischemic stroke (AIS) patients who were not undergoing early recanalization treatments. We aimed to further evaluate the structural MRI characteristics and their clinical significance to better predict and guide tirofiban treatment. In this post hoc analysis of imaging features, the location of infarcts (anterior circulation stroke [ACS] vs posterior circulation stroke [PCS]), degree of cerebral artery stenosis (≤ 69% vs ≥ 70% or occlusion), total infarct volume and ASPECTS score were used to predict the treatment response of tirofiban, defined as a proportion of excellent functional outcome (modified Rankin Scale (mRS) score of 0–1) and favorable functional outcome (mRS score of 0–2) at 90 days. Patients with ACS were more likely to achieve excellent functional outcome (p = 0.004) and favorable functional outcome (p = 0.008) when treated with tirofiban. However, there were no significant differences between the two groups for patients with PCS. For patients due to severe stenosis (≥ 70% or occlusion), there were significant differences between the two groups (p = 0.002 for mRS 0–1; p = 0.011 for mRS 0–2). Meanwhile, we found that tirofiban treatment improved clinical outcome only in patients with ASPECTS (8–10) (p = 0.016). In addition, we found that the effect of tirofiban treatment was independent of total infarct volume. The neuroimaging data supported that patients with ACS and severe stenosis were recommended for the application of tirofiban, and better treatment response could be predicted independent of total infarct volume.
Clinical and Biochemical Assessment of Ozonated Olive Oil Mixed Bone Substitute in Treating Intra Bony Periodontal Defect- Randomized Clinical Trial
Authors: adel nourhan , Taşdemir Zekeriya , Sarhan Susan , Gamal Ahmed 
Published: April 2023

Abstract
Objective

The objective of this study is to investigate the effect of ozonated olive oil in treating intra bony defect by means of clinical and radiographic parameters, and gingival cervicular fluid BMP2 level.

Materials and methods

Thirty periodontitis patients with a deep intra-bony defect. Group I were treated with open flap depridment(OFD) and deproteinized bovine bone(DBB) alone, Group II were treated by OFD and placing DBB with ozonated olive oil, and Group III were treated by OFD and placing DBB with ozonated olive oil and further application of ozone 3 weeks after surgery topically. Clinical parameters were recorded at 3, and 6 months. Radiographic parameters were recorded before surgery and after 6 months. GCF of BMP2 level were recorded before surgery, 1, 3, 7, 14, 21days after surgery.

Results

according to clinical and radiographic parmeters, all groups showed no significant difference between groups. On the basis of GCF of BMP2 level, all groups showed a significant difference between groups in different intervals. Before study until week three, Group II and Group III showed a significant difference (p < 0.025*) according to the percentage change from Group I.

Conclusion

The use of ozonized olive oil in intra-bony defects increase GCF level and availability of bone morphogenic protein 2 significantly compared to the control non ozonized group.

Clinical relevance

ozonated olive oil is working on anabolic and catabolic mediator; it is antimicrobial, immunostimulating, ant hypoxic, and biosynthetic material
A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
Authors: Zita Wayne , Shanmugabalaji Venkatasalam , Ezquerro Miguel , Rodriguez-Concepcion Manuel , Kessler Felix , Glauser Gaetan 
Published: April 2023

Abstract
Background
Isoprenoids are a very large class of metabolites playing a key role in plant physiological processes such as growth, stress resistance, fruit flavor, and color. In chloroplasts and chromoplasts, the diterpene compound geranylgeranyl diphosphate (GGPP) is the metabolic precursor required for the biosynthesis of tocopherols, plastoquinones, phylloquinone, chlorophylls, and carotenoids. Despite its key role for the plant metabolism, reports on GGPP physiological concentrations in planta have been extremely scarce.
Results
In this study, we developed a method to quantify GGPP and its hydrolysis product geranylgeranyl monophosphate (GGP) from tomato fruit, using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Quantification was done by external calibration and the method was validated in terms of specificity, precision, accuracy, and detection and quantitation limits. We further demonstrate the validity of our approach by analysing GGPP contents in the ripe fruits of wild-type tomatoes and mutants defective in GGPP production. Finally, we also show that the sample preparation is key to prevent GGPP hydrolysis and mitigate its conversion to GGP.
Conclusion
Our study provides an efficient tool to investigate the metabolic fluxes required for GGPP supply and consumption in tomato fruit.
D(+)-Camphor as the key phytochemical cue is promising for the trap monitoring in a new monophagous camphor tree borer Pagiophloeus tsushimanus (Coleoptera: Curculionidae)
Authors: Chen Cong , Xu Tian , Li Shouyin , Xue Mingyu , Deng Yadi , Fan Binqi , Yang Chufeng , Hao Dejun , Chen Cong 
Published: April 2023

Abstract
The landscape plant, Cinnamomum camphora, is a broad-spectrum anti-insect tree species due to a diversity of terpenoids, such as D(+)-Camphor. However, C. camphora is selected as the unique host tree species by a monophagous weevil pest, Pagiophloeus tsushimanus. Deciphering the key olfactory signal components regulating its host preference could facilitate the monitoring and control strategies for this insect pest, but which is poorly understood. Herein, two host volatiles, D(+)-Camphor and Ocimene, can induce GC-EAD/EAG reactions in both male and female adults antennae. Correspondingly, Y-tube olfactometer assays showed that the two compounds were attractive to both male and female adults. In the field assays, self-made trap device baited with 5 mg dose D(+)-Camphor captured significantly more P. tsushimanus adults than isopropanol solvent control, but there was no sexual bias in adults captured. The trunk gluing trap device baited with bait can capture adults, but the number is significantly less than that of the self-made trap device and adults often fall after struggling. The cross baffle trap device never traps adults. Neither Ocimene nor isopropanol solvent control could capture adults. When used in combination, Ocimene cannot enhanced the attraction of D(+)-Camphor to both female and male adults. These results indicate that D(+)-camphor is a key active compound of P. tsushimanus adults for host location. Combination of the host-volatile lure based on D(+)-camphor and the self-made trapping device is promising to monitor and control this novel pest P. tsushimanus in C. camphora plantations.
Genomic survey of MYB gene family in six pearl millet(Pennisetum glaucum) varieties and their response to abiotic stresses
Authors: jinhan lv , Xu Yue , Dan Xumin , Yang Yuchen , Mao Chunli , Ma Xixi , Zhu Jie , Min Sun , Jin Yarong , Huang Linkai 
Published: April 2023

Abstract
Besides developmental and metabolic aspects, MYB transcription factors play key roles in plant defence and stress responses. From six pearl millet genomes, 1133 MYB genes were identified, and four subgroups were constructed by phylogenetically. The duplication pattern of MYB genes in the pearl millet genomes has a conserved and similar evolutionary history. In abiotic stress, MYB genes have a preference for drought stress and heat stress and are more strongly regulated in roots. Multiple analyses have shown that MYB genes respond to abiotic stresses by regulating ABA-related, circadian rhythms and histone modification pathways. Under abiotic stress, a large number of duplication events occurred in differentially expressed genes, and the positive expression trend of duplication on PMA5G04432.1-PMA2G00728.1 was consistent across multiple stresses, suggesting that gene duplication is essential for pearl millet adaptation to abiotic stresses. This work will contribute to further studies on the MYB gene family in pearl millet and facilitate further understanding of the potential role of MYB in the regulation of abiotic stresses.
Nutritional status as the risk factor of urosepsis in patients with benign prostatic hyperplasia
Authors: Huang Hao , Ren ZhengJu , Li YaDong , Tan Wei , Wu Hao , Gou YuanQing , Yuan Ye , Liu Chuan 
Published: April 2023

Abstract
Objectives

This study aimed to investigate the risk factors associated with urosepsis in patients with benign prostatic hyperplasia (BPH), particularly focusing on nutritional status.

Methods

We retrospectively analyzed data from 65 BPH patients admitted to our hospital between January 2020 and November 2022 for urosepsis. We also matched 130 BPH patients without urinary tract infection (UTI) as controls with a matching ratio of 1:2. Nutritional status was assessed according to the prognostic nutritional index (PNI) score, the controlling nutrition status (CONUT) score, and the Naples prognostic score (NPS).

Results

Compared with BPH patients without UTI, patients with urosepsis had significantly lower BMI (21.76 ± 2.99 vs. 23.60 ± 3.09, P＜0.001) and PNI score (38.56 ± 7.03 vs. 45.45 ± 5.11, P = 0.003), and higher CONUT score (5.47 ± 2.71 vs. 2.17 ± 1.73, P＜0.001) and NPS (3.55 ± 0.79 vs. 2.35 ± 1.15, P＜0.001). Logistic regression analysis showed that the CONUT score, the NPS, serum albumin, and height were independent and significant risk factors for urosepsis in patients with BPH.

Conclusion

Poor nutritional status may increase the risk of urosepsis in patients with BPH. Therefore, the improvement of nutritional status is conducive to the prevention of severe UTIs in elderly men with BPH.
Spatiotemporal Analysis of Precipitation-based ETCCDI Indicators over India
Authors: Vinod Degavath , Mahesha Amai 
Published: April 2023

Abstract
Identifying trends in hydrometeorological time series during extreme weather events is a significant task. The spatial and temporal fluctuations of precipitation are crucial components of the water cycle and impact water resource management, agriculture, and the environment. Examining Extreme Indices is essential in identifying the effect of climate change on precipitation. In this study, the India Meteorological Department's daily gridded rainfall data (0.250 E and 0.250 N) from 1902-2021 over India were analyzed to investigate 14 precipitation-based Expert Team on Climate Change Detection and Indices (ETCCDI) for climate change at the annual and seasonal time scales across seven climatic zones in India. The non-parametric Mann-Kendall (MK) test with the Theil-slope Sen's estimator and Innovative Trend Analysis (ITA) were utilized to identify and compare the trends and slopes related to the indices with a 5% significance level. The homogeneity of ETCCDI was determined using a non-parametric Pettitte test with a 5% significance level. ITA effectively identified the trends and magnitudes associated with extreme indices seasonally and annually. The study discovered a significant seasonal and annual increase in extreme precipitation indices in India's Ladakh region, which belongs to the arid-desert-cold (BWk) climatic zone. The trends in annual (+23.98%), Southwest (SW) Monsoon (+38.29%), NE Monsoon (+49.80%), and Summer (+74.46%) rainfall increased, while there was a decrease in winter rainfall (-41.84%) trend across most of the climatic zones. Most of the extreme indices exhibited significant variation during 1980-1990. This study provides evidence that climate change is occurring in India by analyzing the pattern of each ETCCDI Indices.
Gender roles perceptions and ideal number of children: case study of Emirati youth
Authors: Shukla Ankita , Karabchuk Tatiana , Neyadi Latifa Mohammed Al 
Published: April 2023

AbstractBackground:United Arab Emirate (UAE) is a very traditional society and a country with declining fertility. Literature suggests that gender-egalitarian attitudes lead to lower fertility. Present research tackles the puzzle of the relationship between perceptions of gender roles in society and ideal number of children among Emirati youths.Methods:This study utilized data from a cross-sectional quantitative survey. The survey was developed and applied in February-March 2019 among a purposive sample of 300 young Emirati males and females aged 18-30 years, studying at the UAE University. Both bivariate and multivariate analysis is performed to examine the levels of youth’s perception towards gender roles and desired fertility.Results:Empirical survey data collected from Emirati youths revealed that 50% of youths support the traditional perspective on marriage, 30% think motherhood is the most important thing for women, and a small percentage support economic independence of women and husband participation in household chores/child-rearing. On average Emirati youth ideally wish to have in their future families 5.4 children, and sons are more preferable for them than daughters. Youths who favour the economic independence of women and equal participation in household work contribution by spouses desire a lower number of children which is in line with the modernization theory and cultural evolution.Conclusion:UAE has been facing continuous decline in fertility rates. Present findings indicate that change in perception towards gender roles maybe one of the factors contributing to fertility decline among young generation. Promoting gender equality attitudes and strengthening institutional support for childbearing could become of the key strategies.
Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study
Authors: Hosseini Hossein , Morshed Rima , Sadri Naser , Kafi Zahra Ziafati , Sarmadi Soroush , Baghi Farhad Arab , Ghalyanchilangeroudi Arash 
Published: April 2023

Abstract
Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes T cell lymphoma in chickens. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. In this study, 30 commercial broiler flocks from different Provinces (10 spleens from each flock) were sampled at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches and molecular features consistent with virulent and very virulent previously identified MDV. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for the molecular surveillance and further study of MDV mutants and strategies plan for control of MD in Iran.