bluuebunny/crossref_metadata_2025_split huggingface dataset:
No detection of SARS-CoV-2 in domestic cats exposed to infected owners during the Omicron Wave in São Paulo, Brazil
Authors: Lopes Luciano Rodrigo , Rigato Paula Ordonhez , Kasinski Silvana , Figueira Liliane Akemi Mizutani , Santos Victor Hugo Vilar Maciel dos , Pina Natalia Carvalho de Lucca , Paiva Paulo Bandiera , Casseb Jorge , Conte Danielle Dias , Bellei Nancy Cristina Junqueira 
Published: June 2023

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been demonstrated to infect various mammals, including cats, dogs, and minks. Given the close coexistence of humans and cats, transmission of SARS-CoV-2 variants between these two species has been documented. This study involved the sampling of eight domestic cats that resided solely with their infected owners in Brazil, during the early stage of the Omicron variant wave. Nasal and anal secretion samples obtained from the eight cats were subjected to RT-qPCR analysis, which revealed no detection of SARS-CoV-2. Additionally, serum samples collected from these cats did not show the presence of anti-SARS-CoV-2 antibodies. The findings of this study indicate that the Omicron variant has a low potential to infect felines, which contributes to the absence of transmission. Therefore, cats do not appear to play a significant role in the transmission chain of SARS-CoV-2.
Research on marginal-restraint mandrel-free spinning of large thin-walled aluminum alloy heads with large bottom reserves
Authors: Zhu Lijun , Huang Cheng , Li Xinhe , Chang Xin , Li Yibo 
Published: June 2023

Abstract
The marginal-restraint mandrel-free spinning method can solve the problem of instability when spin-forming thin-walled head blanks. However, when shear-expansion compound forming is adopted for a large diameter head, the sinking phenomenon that occurs during the expansion spinning stage makes it impossible to accurately form the head. Through finite element simulation, it is found that the smaller the roller fillet radius is, the smaller the feed ratio, and the larger the angle is between passes, the more obvious the sinking phenomenon and the worse the forming accuracy. It is further revealed that the mechanism of the sinking phenomenon is shear deformation in addition to expansion deformation in the expansion spinning stage. To solve the sinking problem, this paper proposes a marginal-restraint mandrel-free spinning method with a large bottom reserve. Research shows that a larger roller fillet radius and more passes can improve the contour accuracy of the head by using the cylindrical roller structure. Finally, it is verified that the new spinning method can achieve accurate forming of large thin-walled aluminum alloy heads with a diameter of 2250 mm and a forming angle of 60°.
Pragmatic clinic-based investigation of echocardiogram parameters in asymptomatic patients with type 2 diabetes in routine clinical practice and its association with suggestive coronary artery disease: A pilot study
Authors: Palma¹ Catia Cristina Silva Sousa Vergara , Lopes² Pablo Moura , Bomfim² Alfredo Souza , Gomes¹ Marilia Brito 
Published: June 2023

Abstract
Background
Patients with Diabetes Mellitus (DM) have cardiovascular diseases (CVD) as a major cause of mortality and morbidity. The primary purpose of the study was to assess the echocardiographic parameters that showed alterations in patients with type 2 diabetes mellitus(T2DM ) with suggestive coronary artery disease (CAD) determined by electrocardiography and the secondary was to assess the relationship of these alterations with established cardiovascular risk factors.
Methods
This cross-sectional, observational pilot study included 152 consecutive patients with T2DM who attended a tertiary diabetes outpatient care center. All patients underwent clinical examination and history, anthropometric measurements, demographic survey, determination of the Framingham global risk score, laboratory evaluation, basal electrocardiogram, echocardiogram, and measurement of carotid intima-media thickness (CIMT).
Results
From the overall sample, 134 (88.1%) patients underwent an electrocardiogram. They were divided into two groups: patients with electrocardiograms suggestive of CAD (n = 11 [8,2%]) and those with normal or non-ischemic alterations on electrocardiogram (n = 123 [91,79%]). In the hierarchical multivariable logistic model examining all selected independent factors entered into the model, sex, high triglyceride levels, and presence of diabetic retinopathy were associated with CAD in the final model. No echocardiographic parameters were significant in the multivariate analysis.
Conclusion
Our pilot study demonstrated that no echocardiogram parameters could predict or determine CAD. The combination of CIMT and Framingham risk score is ideal to determine risk factors in asymptomatic patients with T2DM. Patients with diabetic retinopathy and hypertriglyceridemia need further investigation for CAD. Further prospective studies with larger sample sizes are needed to confirm our results.
Ecological attributes promoting intra-continental range-expansion of a native annual forb triggered by intensified land-use
Authors: Ruprecht Eszter , Essl Franz , Moț Augustin C. , Balaji Beatrix , Kuhn Thomas , Fenesi Annamária , Mardari Constantin , Miholcsa Zsombor 
Published: June 2023

Abstract
Intra-continental range-expansion of native species tracking human-induced environmental changes has become an important phenomenon, and recently has been suggested to be recognized as a distinct biogeographic category, namely neonative. However, it remains difficult to analyze how range-expansions are triggered by certain environmental changes and which biological and ecological species attributes are involved. We compared biological and ecological attributes and the performance in different test environments during the entire life cycle of a range-expanding neonative in Romania, Xeranthemum cylindraceum, with a congeneric resident native, X. annuum, through a series of controlled experiments. Both species proved to share several biological and ecological attributes, e.g. high potential for long-distance dispersal by multiple vectors, biochemical defence against herbivores by means of cyanogenic compounds, and surprisingly, they were found to have similar germination and performance niches throughout the range of climatic environments tested by us. Differences between the two species, a broader climatic and habitat niche, higher germination rate and viability of seeds can give explanation to the successful spread beyond the historical range of X. cylindraceum. However, based on shared biological attributes between the two species, which confer the potential to spread, and the considerably higher seed output found in the case of the resident native, X. annuum is equally prepared to expand its range. With changing climate and persisting human-induced disturbance, in this case intensive grazing by sheep, we may expect the spread of this species after passing the presumed migration lag phase.
Synthesis and Raman Performance Enhancement of Multilayer AuAg Heterostructures with Magnetic Resonance
Authors: Huang Fan , Zhao Zhi-Rui , Feng Jing-Ru , Ma Liang , Ding Si-Jing 
Published: June 2023

Abstract
Significant amplification of surface enhanced Raman scattering (SERS) signals can be achieved mainly by the electric field enhancement in metal core-shell nanostructures, and the enhanced magnetic field is rarely studied. In this study, our team prepared multi-gap Au/AgAu core-shell hybrid nanostructures by using gold nanocup as the core. The overgrowth processes to grow one, two, and three layers of AgAu hybrid nanoshells can produce Au/AgAu1, Au/AgAu2, and Au/AgAu3 heteronanostructures. The strong plasmon coupling between the core and shell leads to significant electromagnetic field enhancement. Under the synergistic effect of electromagnetic plasmon resonance and plasmon coupling, Au/AgAu core-shell hybrid nanostructures exhibit excellent SERS signals. We also investigate the effect of the interstitial position of the rhodamine B (RhB) molecule on Raman enhancement in Au/AgAu3 heteronanostructures. Our research can provide new ideas for the synthesis of multi-gap Raman signal amplifiers based on magnetic plasmon coupling.
Prognostic Value and Mode Selection of Locoregional Treatment in Stage-IV Breast Cancer Patients
Authors: Cui Hanxiao , Ren Xueting , Zhao Xuyan , Dai Luyao , Liu Dandan , Bao Yuanhang , Hu Liqun , Xiao Zhengtao , Ma Xiaobin , Kang Huafeng 
Published: June 2023

Abstract
Purpose: This study aimed to assess the actual prognostic significance of different LRT (surgery and radiotherapy) modalities for stage-IV BC patients, and construct a competing risk nomogram to make precise predictions of the breast cancer-specific death (BCSD) risk among LRT recipients.
Methods: A total of 9279 eligible stage-IV BC patients from the Surveillance Epidemiology and End Results (SEER) database were included in this study. Initially, we evaluated the impact of LRT on survival both before and after the propensity score matching (PSM). Then, we used the Cox hazard proportional model and competing risk model to identify the independent prognostic factors for LRT recipients. Based on the screened variables, a comprehensive nomogram was established.
Results: Kaplan-Meier curves demonstrated that LRT significantly prolonged overall survival (OS) and breast cancer-specific survival (BCSS) (P<0.001). In addition, patients treated with surgery combined with postoperative radiotherapy (PORT) possessed the optimal survival (P<0.001). Regardless of the surgical modalities, primary tumor resection combined with radiotherapy could ameliorate the prognosis (P<0.05). Subgroup analysis showed that in patients with T2-T4 stage, PORT had a survival benefit compared with those undergoing surgery combined with preoperative radiotherapy (PRRT) and surgery only. Based on the screened independent prognostic factors, we established a comprehensive nomogram to forecast BCSD in 1-, 2-, and 3-year, which showed robust predictive ability.
Conclusion: PORT was associated with a lower BCSD in stage-IV BC patients. The practical nomogram could provide a precise prediction of BCSD for LRT recipients, which was meaningful for patients’ individualized management.
Human antimicrobial peptide Histatin 1, 3, and its autoproteolytic cleaved peptides target the monkeypox virus surface proteins: molecular modelling and docking studies
Authors: Radhakumar Deeksha SarojiniDevi , Thiyagarajan Sundar , Rajaram Kaushik , Parsanathan Rajesh 
Published: June 2023

Abstract

ContextMonkeypox is a viral zoonotic illness resembling smallpox. It was a national epidemic, primarily in Africa, but the recent outbreak of the monkeypox virus (MPXV) which affected numerous countries, including India. MPXV surface proteins are considered important targets, since these are involved in the virus entry into a host and cell-cell fusion(syncytial formation). Here, we suggest a novel antimicrobial peptide therapy-based method for treating MPXV infection. Anin silicomethod was used to analyze the human antimicrobial peptides Histatin 1, 3, and its autoproteolytic cleaved products (hAMP HIS), which bind to several MPXV surface proteins. Compared to HIS 1 and 3, HIS 5 has higher solubility, estimated half-life, cell permeability, stability, and good docking score. Hence, based on the physicochemical properties of HIS 5, it may be used as a novel potential therapeutic peptide against MPXV. Current study shows hAMP HIS family may effectively target the MPXV. It would be fascinating to conduct more research on the HIS 5 peptide's effects on viral infections in bothin vitroandin vivomodels.MethodsMPXV surface proteins and hAMP HIS localization and 3D structures was predicted by Protter, and trRosetta & c-quark respectively; 3D model refinement and validated carried out using the GalaxyRefine, Errat, Procheck, and Qmean (SwissModel). Further, molecular docking was carried out for MPXV surface proteins with hAMP HIS using HDOCK and Cluspro 2.0. Protein-peptide interactions were analyzed using PdbSum. Finally, physico-chemical property of HIS peptides was carried out using CamSol.
Potential mechanisms of radiation-induced esophageal tissue injury in TUT4 -/- mice
Authors: Ren Huiwen , Fan Zhigang , Sun Zhiqiang , Liu Jun , Fang Mingming , Luo Judong 
Published: June 2023

Abstract
Background
Radiation-induced esophageal injury remains a limitation of radiotherapy for lung and esophageal cancer patients. Esophageal epithelial cells are extremely sensitive to irradiation; nevertheless, factors involved in the radiosensitivity of esophageal epithelial cells are still unknown. Terminal uridyl transferase 4 (TUT4) can modify the sequence of miRNAs, which affects their regulation of miRNA targets and function.
Methods
In this study, we used transcriptome sequencing technology to identify mRNAs that were differentially expressed before and after radiotherapy in esophageal epithelial cells.
Results
We further explored the mRNA expression profiles between wild-type and TUT4 knockout esophageal epithelial tissue. Volcano and heatmap plot unsupervised hierarchical clustering analyses were performed to classify the samples. Enrichment analysis of Gene Ontology (GO) functional annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was performed. Annotation of differential genes from metabolism, genetic information processing, environmental information processing, cellular processes and organismal systems human diseases was conducted.
Conclusions
The aberrantly expressed genes were significantly enriched in irradiation-related biological processes, such as lipid metabolic process, fatty acid metabolic process, proteolysis, and metabolic process. Moreover, we explored the distribution of the transcription factor family and its target genes among the differentially expressed genes. In addition, we found that PPAR pathway and the renin-angiotensin pathway might serve as therapeutic targets in TUT4-related radiation-induced esophageal injury.
Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye
Authors: DISLI Hüseyin Burak , HIZLISOY Harun , GUNGOR Candan , BAREL Mukaddes , DISHAN Adalet , GUNDOG Dursun Alp , AL Serhat , ONMAZ Nurhan ERTAS , YILDIRIM Yeliz , GONULALAN Zafer 
Published: June 2023

Abstract
Aliarcobacter spp. have has been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were: i) to isolate Aliarcobacter species (Aliarcobacter butzleri, Aliarcobacter skirrowii, Aliarcobacter cryaerophilus) from different slaughterhouses’ samples, ii) to detect genetic diversity, antibiotic resistance, biofilm ability and putative virulence gene profiles of the isolates. The antibiotic susceptibility to antimicrobials was examined using the Kirby-Bauer disc diffusion method. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR). A total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. In terms of virulence factors, all isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three. Regarding biofilm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate biofilms, respectively, while one (4.5%) isolate was classified as a weak biofilm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These findings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food, and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are significant sources of antimicrobial resistant Aliarcobacters.
Anxiety and Depression in Pediatric-Onset Traumatic Spinal Cord Injury: A Systematic Review
Authors: Mofatteh Mohammad , Mashayekhi Mohammad Sadegh , Arfaie Saman , Chen Yimin , Malhotra Armaan K. , Skandalakis Georgios , Alvi Mohammed Ali , Afshari Fardad T. , Meshkat Shakila , Abdulla Ebtesam , Anand Ayush , Liao Xuxing , McIntyre Roger S. , Santaguida Carlo , Weber Michael H. , Fehlings Michael G. 
Published: June 2023

Abstract
Background and aim
Traumatic spinal cord injury (TSCI) is a debilitating neurological condition with significant long-term consequences on the mental health and wellbeing of affected individuals. We aimed to investigate anxiety and depression in individuals with pediatric-onset TSCI.
Methods
PubMed, Scopus, and Web of Science databases were searched from inception to December 20th, 2022 following the PRISMA guidelines and studies were included according to the eligibility criteria.
Results
1,013 articles were screened, and 18 studies with 4,234 individuals were included in the final review. 1,613 individuals (38.1%) had paraplegia, whereas 1658 (39.2%) had tetraplegia. 1,831 participants (43.2%) had complete TSCI, whereas 1024 (24.2%) had incomplete TSCI. The most common etiology of TSCI with 1,545 people (36.5%) was motor vehicle accidents. The youngest mean age at the time of injury was 5.92 ± 4.92 years, whereas the oldest was 14.6 ± 2.8 years. Patient Health Questionnaire-9 was the most common psychological assessment used in 9 studies (50.0%). Various risk factors, including pain in 4 studies (22.2%), reduced sleep quality, reduced functional independence, illicit drug use, incomplete injury, hospitalization, reduced quality of life, and duration of injury in 2 (11.1%) studies, each, were associated with elevated anxiety and depression.
Conclusions
Different biopsychosocial risk factors contribute to elevated rates of anxiety and depression among individuals with pediatric-onset TSCI. Individuals at risk of developing anxiety and depression should be identified, and targeted support should be provided. Future large-scale studies with long-term follow-up are required to validate and extend these findings.
Computational Approach for Molecular Design of Small Organic Molecules with High Hole Mobilities in Amorphous Phase using Random Forest Technique and Computer Simulation Method
Authors: Nakaguro Keijin , Mitsuta Yuki , Koseki Shiro , Oshiyama Tomohiro , Asada Toshio 
Published: June 2023

Abstract
The molecular design system of small organic molecules was developed to realize high hole mobility in the amorphous phase by using the random forest technique and the computer simulation method. Since there was no accessible datasets of the hole mobilities in the amorphous phase, we have constructed a datasets of experimental hole mobilities for 321 organic molecules with calculated MO energies to utilize machine learning techniques for molecular generation procedures. Random forest (RF) was applied to the machine learning technique using the constructed datasets. The optimized RF with 69 structure-based descriptors exhibited correlation coefficients of 0.885 and 0.764 for the training and testing datasets, respectively. RF confirmed that molecules with two triphenylamine (TPA) and/or carbazole groups are likely to display high hole mobilities according to the topological polar surface area (TPSA). The modified ChemTS to use optimized RF was adapted to design molecules with high hole mobilities. The SC model that uses ab initio molecular orbital (MO) calculations and the Marcus theory was adapted to ensure the predicted mobilities. The generated molecules were verified to have high calculated hole mobility (the order of \({10}^{-2} \text{c}{\text{m}}^{2}/\text{V}\text{s})\) owing to the high transfer integral and low reorganization energy by the successive conduction (SC) model. The datasets and programs used to design molecules in this manuscript were publicity released on GitHub.
Design and Implementation of Twin-manipulator Material Sorting System Based on ResNet18
Authors: Li Lipeng , Liu Jishen , Ding Mei , Yang Lu , Mo Yicong 
Published: June 2023

Abstract
In order to solve the problem of time-consuming, labor-intensive and inefficient traditional packaging and sorting operations, an intelligent sorting system is designed based on machine vision theory and manipulator control technology. This paper introduces the design and implementation strategy of intelligent material sorting system in detail, and expounds the design and implementation of visual processing based on Res Net18 neural network and mechanical arm loading, communication, identification and sorting procedures under the framework of Python. The effectiveness and accuracy of the system are verified based on specific experiments. In the sorting test of four kinds of stacking materials, the recognition time is within 100ms, and the recognition accuracy rate is more than 99%, which meets the system design requirements. During the whole process, the intelligent material sorting system operates smoothly without problems such as holding brake and material dropping, and the sorting and grasping accuracy is high, which meets the requirements of high accuracy and high stability in the sorting field.
Shifting vegetation phenology in eddy covariance data: Methodological challenges and new perspectives
Authors: Panwar Annu , Migliavacca Mirco , Nelson Jacob A. , Cortes Jose , Bastos Ana , Forkel Matthias , Winkler Alexander J. 
Published: June 2023

Abstract

Numerous studies report shifts in vegetation phenology, however, in this regard eddy covariance (EC) data is still not fully exploited despite their continuous high-frequency observations. Moreover, there is no general consensus on optimal methodologies for data smoothing and extracting phenological transition dates (PTDs). Here, we revisit existing methodologies and present new prospects to investigate phenological changes in Gross Primary Productivity (GPP) from EC measurements. First, we present a smoothing technique of GPP time series through the derivative of its smoothed annual cumulative sum. Second, we calculate PTDs and their trends from commonly used threshold method that identifies days with a fixed percentage of the annual maximum GPP. A systematic analysis is performed for various thresholds ranging from 0.1 to 0.7. Lastly, we examine the relation of PTDs trends to trends in GPP across the years on a weekly basis. Results from 47 EC sites with long time series (> 10 years) show that advancing trends in start of season (SOS) are strongest at lower thresholds but for the end of season (EOS) at higher thresholds. Moreover, the trends are variable at different thresholds for individual vegetation types and individual sites, outlining reasonable concerns on using a single threshold value. Relationship of trends in PTDs and weekly GPP reveal association of advanced SOS and delayed EOS to increase in immediate primary productivity, but not to the trends in overall seasonal productivity. Drawing on these analyses, we emphasise on abstaining from subjective choices and investigating relationship of PTDs trend to finer temporal trends of GPP. Our study examines existing methodological challenges and presents approaches that optimize the use of EC data in identifying vegetation phenological changes and their relation to carbon uptake.