bluuebunny/crossref_metadata_2025_split huggingface dataset:
The transcription factor ERF108 interacts with AUXIN RESPONSE FACTORs to mediate cotton fiber secondary cell wall biosynthesis
Authors: Wang Yao , Li Yang , He Shao-Ping , Xu Shang-Wei , Li Li , Zheng Yong , Li Xue-Bao 
Published: August 2023

Abstract
Phytohormones play indispensable roles in plant growth and development. However, the molecular mechanisms underlying phytohormone-mediated regulation of fiber secondary cell wall (SCW) formation in cotton (Gossypium hirsutum) remain largely underexplored. Here, we provide mechanistic evidence for functional interplay between the APETALA2/ethylene response factor (AP2/ERF) transcription factor GhERF108 and auxin response factors GhARF7-1 and GhARF7-2 in dictating the ethylene–auxin signaling crosstalk that regulates fiber SCW biosynthesis. Specifically, in vitro cotton ovule culture revealed that ethylene and auxin promote fiber SCW deposition. GhERF108 RNA interference (RNAi) cotton displayed remarkably reduced cell wall thickness compared with controls. GhERF108 interacted with GhARF7-1 and GhARF7-2 to enhance the activation of the MYB transcription factor gene GhMYBL1 (MYB domain-like protein 1) in fibers. GhARF7-1 and GhARF7-2 respond to auxin signals that promote fiber SCW thickening. GhMYBL1 RNAi and GhARF7-1 and GhARF7-2 virus-induced gene silencing (VIGS) cotton displayed similar defects in fiber SCW formation as GhERF108 RNAi cotton. Moreover, the ethylene and auxin responses were reduced in GhMYBL1 RNAi plants. GhMYBL1 directly binds to the promoters of GhCesA4-1, GhCesA4-2, and GhCesA8-1 and activates their expression to promote cellulose biosynthesis, thereby boosting fiber SCW formation. Collectively, our findings demonstrate that the collaboration between GhERF108 and GhARF7-1 or GhARF7-2 establishes ethylene–auxin signaling crosstalk to activate GhMYBL1, ultimately leading to the activation of fiber SCW biosynthesis.
The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize
Authors: Chen Erwang , Yu Huiqin , He Juan , Peng Di , Zhu Panpan , Pan Shuxing , Wu Xu , Wang Jincang , Ji Chen , Chao Zhenfei , Xu Zhuopin , Wu Yuejin , Chao Daiyin , Wu Yongrui , Zhang Zhiyong 
Published: August 2023

Abstract
Endosperm filling in maize (Zea mays), which involves nutrient uptake and biosynthesis of storage reserves, largely determines grain yield and quality. However, much remains unclear about the synchronization of these processes. Here, we comprehensively investigated the functions of duplicate NAM, ATAF1/2, and CUC2 (NAC)-type transcription factors, namely, ZmNAC128 and ZmNAC130, in endosperm filling. The gene-edited double mutant zmnac128 zmnac130 exhibits a poorly filled kernel phenotype such that the kernels have an inner cavity. RNA sequencing and protein abundance analysis revealed that the expression of many genes involved in the biosynthesis of zein and starch is reduced in the filling endosperm of zmnac128 zmnac130. Further, DNA affinity purification and sequencing combined with chromatin-immunoprecipitation quantitative PCR and promoter transactivation assays demonstrated that ZmNAC128 and ZmNAC130 are direct regulators of 3 (16-, 27-, and 50-kD) γ-zein genes and 6 important starch metabolism genes (Brittle2 [Bt2], pullulanase-type starch debranching enzyme [Zpu1], granule-bound starch synthase 1 [GBSS1], starch synthase 1 [SS1], starch synthase IIa [SSIIa], and sucrose synthase 1 [Sus1]). ZmNAC128 and ZmNAC130 recognize an additional cis-element in the Opaque2 (O2) promoter to regulate its expression. The triple mutant zmnac128 zmnac130 o2 exhibits extremely poor endosperm filling, which results in more than 70% of kernel weight loss. ZmNAC128 and ZmNAC130 regulate the expression of the transporter genes sugars that will eventually be exported transporter 4c (ZmSWEET4c), sucrose and glucose carrier 1 (ZmSUGCAR1), and yellow stripe-like2 (ZmYSL2) and in turn facilitate nutrient uptake, while O2 plays a supporting role. In conclusion, ZmNAC128 and ZmNAC130 cooperate with O2 to facilitate endosperm filling, which involves nutrient uptake in the basal endosperm transfer layer (BETL) and the synthesis of zeins and starch in the starchy endosperm (SE).
Hydrogen Sulfide Attenuates Mesenchymal Stem Cell Aging Progress via the Calcineurin-NFAT Signaling Pathway
Authors: Yang Shan , Su Yingying , Li Xiaoyan , Wang Hao , Du Juan , Feng Jie , Xie Yongmei , Luo Zhenhua , Xu Junji , Guo Lijia , Liu Yi 
Published: August 2023

Abstract
Aging is a gradual process that is coupled with a decline in the regenerative capacity of stem cells and a subsequent reduction in tissue function and repair. Hydrogen sulfide (H2S) plays an important role in maintaining the function of stem cells. The present study aimed to investigate the role of H2S in mesenchymal stem cell aging and the underlying mechanism and to provide novel insights into stem cell therapies in elderly people. Bone marrow mesenchymal stem cells (BMMSCs) were isolated from young mice (2 months) and from old mice (12 months). Senescence-associated β-galactosidase (SA-β-Gal) activity, reactive oxygen species (ROS) production, ROS scavenging enzymes, and the expression of cell-cycle-related genes were compared between those young and old BMMSCs. The expression of H2S-producing enzymes and the production of H2S in BMMSCs were examined. In vitro osteogenic differentiation and cell senescence were analyzed in young and old BMMSCs before and after H2S treatment. The underlying mechanism was investigated using calcineurin and NFAT1 inhibitors or a Foxp3 siRNA. Bone volume/tissue volume (BV/TV) of femurs in mice was examined using micro-CT with or without systemic injection of an H2S donor. Here, we found that H2S levels in BMMSCs declined with age. When the generation of H2S was blocked with the CBS inhibitor hydroxylamine and the CSE inhibitor dl-propargylglycine, BMMSCs underwent senescence. The elevation of H2S levels rescued BMMSC function in vitro and prevented bone loss in vivo. Mechanistically, H2S represses cell aging via the calcineurin-NFAT1 signaling pathway.
Leveraging an implementation science partnership network to understand how Federally Qualified Health Centers operationalize and address health equity
Authors: Aschbrenner Kelly A , Cruz Jennifer L , Kruse Gina R , Nguyen Huy , Huebner Torres Cristina , Celli Maria , Sarcione Carrie , Singh Deepinder , Emmons Karen M 
Published: August 2023

Abstract
Health equity-focused implementation research requires using definitions and approaches that are relevant and meaningful to implementation partners. We examined how health equity was operationalized and addressed at Federally Qualified Health Centers (FQHCs). We conducted semi-structured interviews with leadership (n = 19) and staff (n = 12) at 10 FQHCs in an implementation science partnership network for cancer control equity to understand how they operationalized and addressed health equity. We performed rapid qualitative analysis and shared findings with a larger group of 13 community health centers (including the 10 FQHCs) at an Implementation Learning Community (ILC) to identify action areas for research and practice, followed by a second phase of synthesizing qualitative codes into themes and mapping themes onto a framework for advancing health equity in healthcare organizations. Participants defined health equity as central to the mission of FQHCs, and identified barriers (e.g. financing models) and facilitators (e.g. interpreter services) to advancing health equity at FQHCs. These findings resonated with ILC participants who emphasized the challenge of addressing root cause social determinants of inequities using limited available resources in FQHCs and the importance of developing meaningful collaboration with communities for data collection, data interpretation, data use, and data ownership. Themes captured recommendations to advance health equity in daily work at FQHCs, including investments in staffing, training, and resources. Mapping qualitative themes from health equity-centered interviews with FQHC partners onto a framework for advancing health equity in healthcare organizations can provide clear, context-specific direction for actions aimed at improving health and healthcare equity.
Predicting Potato Diseases in Smallholder Agricultural Areas of Nigeria Using Machine Learning and Remote Sensing-Based Climate Data
Authors: Ibrahim Esther S. , Nendel Claas , Kamali Bahareh , Gajere Efron N. , Hostert Patrick 
Published: August 2023

Crop disease management is crucial for sustainable food production. Although farmers in Nigeria continue to apply broad-spectrum fungicides to potato, potato diseases are still on the rise. Machine-learning methods have recently become more common as part of epidemiological early warning systems. They provide vital information on data–disease relations that may be useful for predisease management. In this study, we build on machine-learning methods to develop spatial early warning tools for Nigeria, using the Jos Plateau as a test case. Both remote sensing meteorological and field data were used to (i) predict disease incidence using field reference data and a random forest (RF) classifier and to (ii) identify local conditions conducive to potato diseases using multi-criteria classification (MCC) based on machine-learning results. The results of the RF for 2019, 2020, and 2021 showed similar spatial characteristics, whereas the MCC varied significantly. Both models predicted that between 72 and 96% of potato fields would be infested. The MCC model further revealed that spatiotemporal frequencies of vulnerability in June can serve as the indicator that informs degrees of infestation. A 5-day vulnerability window used in the context of the MCC proved to be the most useful tool for developing an efficient spraying regime, based on a combination of temperature, rainfall, and relative humidity thresholds. As a result, we were able to develop an operational early-warning system for potato disease in the tropical highlands of Africa. In particular, we introduced spatial risks, creating a more sustainable early-warning approach. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Do We Need Support in Prepectoral Breast Reconstruction? Comparing Outcomes with and without ADM
Authors: Salibian Ara A. , Bekisz Jonathan M. , Kussie Hudson C. , Thanik Vishal D. , Levine Jamie P. , Choi Mihye , Karp Nolan S. 
Published: August 2023

Background:
The majority of two-stage prepectoral breast reconstruction has been described utilizing acellular dermal matrix (ADM). Although reports of prepectoral breast reconstruction without ADM exist, there is a paucity of comparative studies.

Methods:
A single-institution retrospective review was performed of consecutive patients undergoing immediate prepectoral two-stage breast reconstruction with tissue expanders from 2017 to 2019. Short-term reconstructive and aesthetic complications were compared between cases that utilized ADM for support and those that did not.

Results:
In total, 76 cases (51 patients) were identified, of which 35 cases utilized ADM and 41 did not. Risk factors and demographics were similar between the two cohorts with the exception of body mass index, which was higher in the ADM cohort (29.3 versus 25.4, P = 0.011). Average follow-up length was also longer in patients who received ADM (20.3 versus 12.3 months, P < 0.001). Intraoperative expander fill was higher in patients who did not receive ADM (296.8 cm3 versus 151.4 cm3, P < 0.001) though final implant size was comparable in both cohorts (P = 0.584). There was no significant difference in the rate of any complication between the ADM and no ADM cohorts (25.7% versus 17.1%, respectively P = 0.357), including major mastectomy flap necrosis (P = 0.245), major infection (P = 1.000), seroma (P = 0.620), expander explantation (P = 1.000), capsular contracture (P = 1.000), implant dystopia (P = 1.000), and rippling (P = 0.362).

Conclusions:
Immediate two-stage prepectoral breast reconstruction with tissue expanders has comparable rates of short-term complications with or without ADM support. Safety of prepectoral expander placement without ADM may warrant more selective ADM use in these cases.
Outcomes of Implant Removal and Capsulectomy for Breast Implant Illness in 248 Patients
Authors: Katsnelson Jacob Y. , Spaniol Joseph R. , Buinewicz Joshua C. , Ramsey Frederick V. , Buinewicz Brian R. 
Published: August 2023

Background:
Breast implant illness (BII) is a term popularized by social media to describe systemic symptoms that patients ascribe to their breast implants. Though the concept of implants as an underlying cause for a systemic illness remains controversial, few studies have delineated the implant characteristics, capsular histology, and outcomes of patients who undergo explantation for BII.

Methods:
We retrospectively reviewed the demographics, presenting symptoms, outcomes, capsular histology, and culture results of all women who presented to the senior author with symptoms attributed to BII and underwent breast implant removal with capsulectomy from August 2016 to February 2020. Chi-square and logistic regression analyses were performed to evaluate association between implant type, composition, and findings of inflammation on capsule pathology.

Results:
Among 248 patients, 111 (23%) capsules demonstrated inflammatory changes on permanent pathology. Capsular inflammation was independently associated with silicone versus saline (right odds ratio [OR] = 2.18 [1.16–4.11], P = 0.016, left OR = 2.35 [1.08–5.12], P = 0.03) and textured versus smooth implants (right OR = 2.18 [1.16–4.11], P = 0.016, left OR = 2.25 [1.17–4.31], P = 0.01). Silicone material was present in the capsules of 12 patients (4.8%). Fourteen patients had positive cultures. There was one pneumothorax (0.4%), three hematomas requiring evacuation (1%), and two DVTs (0.8%). Of 228 patients, 206 (90.4%) reported high satisfaction with the outcome of the procedure.

Conclusions:
In a large cohort of BII patients, we found that capsular inflammation is significantly associated with silicone and textured implants. Implant removal with capsulectomy can be safely performed in patients with BII with a low complication rate and high patient satisfaction.
A fearful adult attachment style is associated with double the prevalence of chronic pain compared to secure attachment: A national survey of a South African population
Authors: Stamp Gabriella Elisabeth , Iacovides Stella , Wadley Antonia Louise 
Published: August 2023

AbstractOur response to threats, including pain, are believed to be learnt during our early interpersonal relationships, and can be measured through attachment style. Preliminary epidemiological evidence suggests that insecure attachment styles are more prevalent in those with chronic pain. Our aim was to determine the association between adult attachment style and chronic pain prevalence and burden in a South African population. A nationwide online survey determined adult attachment style (using The Experience in Close Relationships - Relationship Structures (ECR-RS) Questionnaire), prevalence of chronic pain and typically-associated psychological factors. In those with chronic pain, the association with attachment style and pain burden (pain sites, severity and interference, using the Brief Pain Inventory) was further determined. Results of the 2371 individuals were analyzed using multivariate generalized linear models. In our young (median age 23 years; IQR 20-28), well-educated and primarily female (74%) cohort with predominantly a middle-to-high socioeconomic status, we found a high prevalence of chronic pain (27%). All three insecure attachment styles were associated with increased chronic pain prevalence when compared to the secure attachment style (Dismissing: 31%, Odds ratio [95%CI] = 1.38 [1.02-1.85], p=0.037; Preoccupied: 42%, Odds ratio [95%CI] = 2.26 [1.62-3.13], p<0.001; Fearful: 49%, Odds ratio [95%CI] = 2.95 [2.03-4.29], p<0.001). Adult attachment style was not directly associated with the burden of chronic pain, because pain catastrophizing mediated this relationship. Adult attachment style was, however, directly associated with chronic pain prevalence, with more than double the chronic pain prevalence in the fearfully, compared to securely, attached individuals.
Cytokines as Potential Novel Therapeutic Targets in Severe Inflammatory Cardiomyopathy
Authors: Suwalski Phillip , Golpour Ainoosh , Weiner January , Musigk Nicolas , Balzer Felix , Giesa Niklas , Amr Ali , Trebing Johannes , Sedaghat-Hamedani Farbod , Meder Benjamin , Beule Dieter , Landmesser Ulf , Heidecker Bettina 
Published: August 2023

ABSTRACTBackgroundDespite currently available state-of-the art therapies, a substantial proportion of patients with inflammatory cardiomyopathy progresses to advanced heart failure. There is an urgent need for novel therapies to improve outcomes. We hypothesized that elevated cyto-kine levels in inflammatory cardiomyopathy may lead to cardiac injury and that specific cyto-kines are associated with severely decreased left ventricular function consequently, thereby suggesting their potential as therapeutic targets.Methods and ResultsBlood samples collected from 529 patients at 2 registries were inves-tigated. First, in a derivation cohort of inflammatory cardiomyopathy from our medical center (n=63), we discovered cytokines that correlate inversely with severely decreased left ventricu-lar ejection fraction (LVEF). We confirmed reproducibility of our results in an independent cohort from a national registry (n=425) and to some degree generalizability in a small cohort of idiopathic dilated cardiomyopathy (IDCM, n=41). In total, we identified 82 cytokines asso-ciated with severely decreased LVEF (FDR < 0.05); a small portion had been previously pro-posed as therapeutic targets, while others emerged as novel discoveries. Finally, real-world data from electronic medical records further indicated the potential of inhibitors targeting cy-tokines of interest to confer a cardioprotective effect.ConclusionsWe identified 82 cytokines associated with severe inflammatory cardiomyopa-thy. Our data were highly significant, reproducible, and generalizable to IDCM. The fact that some of the cytokines had been suggested as potential targets in prior literature supports va-lidity and plausibility of our data. Given that inhibition of cytokines is technically feasible, the identified proteins are compelling potential novel therapeutic targets.Trial registration number:ClinicalTrials.govIdentifier:NCT04265040,NCT02187263VISUAL ABSTRACT
Secular Trends in Airway Management of Out-of-Hospital Cardiac Arrest in the National EMS Information System (NEMSIS) Dataset
Authors: Gage Christopher B. , Powell Jonathan R. , Nassal Michelle , Wang Henry , Panchal Ashish R. 
Published: August 2023

AbstractIntroductionAdvanced airway management is essential in resuscitation from out-of-hospital cardiac arrest (OHCA). No longitudinal national studies have described longitudinal trends in airway device choice. We sought to evaluate secular trends of OHCA endotracheal intubation (ETI) and supraglottic airway (SGA) in the United States (US).MethodsWe evaluated ETI and SGA use for 2013-2022 in adult OHCA in the US using the National EMS Information System (NEMSIS) database. We identified OHCA events (CPR performed or defibrillation) and evaluated the proportions of ETI and SGA used during OHCA. We repeated the results stratified by urbanicity. We used descriptive statistics to describe incidence prevalence with nonparametric trend testing for proportional changes over time and a two-sample stochastic rank sum test for equivalence to evaluate airway use differences by urbanicity.ResultsDuring the study period, we observed 320,154,097 adult 9-1-1 events. Of 3,118,703 OHCA, there were 699,568 and 337,458 cases with reported ETI and SGA attempts. The dominant airway choice was ETI, though the trend of ETI choice decreased as SGA increased over time (p-trend <0.05). From 2013 to 2022, SGA use increased in urban settings while rural and suburban remained stable (urban 27% to 39%*; suburban 31% to 29%; rural 28% to 29%, respectively, *p<0.05).ConclusionOver ten years, rates of advanced airway use have increased, with ETI remaining the predominant airway for adults in OHCA. Interestingly, ETI choice decreased as SGA increased over the study period. SGA use distinctly differed in urban settings, increasing concerns for disparities in care provision among communities. With the increased use of SGA over time, further evaluation of patient outcomes is required in datasets with robust linkage to Utstein variables.
Pediatric solid pseudopapillary neoplasm of the pancreas
Authors: Samuel Ashish Sam , Boddu Deepthi , Sebastian Patricia , Thomas Alex , Sreekanth K T , Hegde Priyanka , Jehangir Susan 
Published: August 2023

AbstractBackgroundSolid pseudopapillary neoplasm of the pancreas (SPN) in children is rare tumor with low malignant potential. Some tumors however behave aggressively. There is very little literature on the management of these variants especially in children. We share our experience of managing large and recurrent SPN and explore the clinicopathological findings correlating to the risk of recurrence.MethodsThis is a retrospective study of children who were treated for SPN between 2012 and 2022 at a tertiary care center in India. The clinicopathological features and management strategies in these children were evaluated.Results16 children with SPN were treated during this period (88% girls). The median age of presentation was 12 years (IQR 9-14). All children presented with abdominal pain. Computed tomography gave a definitive diagnosis in 81% of cases. The tumor predominantly involved the head of the pancreas (n=9, 56%). Eight of nine children classified as high-grade malignant had a benign course. One child had a recurrence of the tumor 4 years after the initial resection and further recurrence on chemotherapy. She required radiation therapy in addition to reoperation following which she is disease free for 77 months. The overall median follow-up was 46 months (IQR 18-72 months).ConclusionComplete resection of the tumor provides a cure in most patients with SPN. Recurrent tumors require a multi-modality approach. Long-term survival is good. Better prognostic criteria with immunohistochemistry are required to predict the behavior of these tumors as the WHO criteria for malignancy correlate poorly with clinical outcomes in childhood SPN.