bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fortified milk-beverage with amphora algae and its functionality for aflatoxin inactivation in rats
Authors: Badr Ahmed Noah , Abdel-Salam Ahmed M. , Zaghloul Ahmed H. , Farrag Abdel Razik H. 
Published: December 2022

AbstractAflatoxins are considered a severe hazard, contaminate dietary products, and cause malignant alterations in liver tissues. Fermented milk (FM) is prepared using probiotic lactic acid strains. This investigation aimed to produce an integrated milk beverage, inactivating aflatoxins toxicity and biotransformation. The proximate analysis of the investigated materials and biochemical parameter changes of the in-vivo experiment were determined. Results reflected the extract’s valuable content of polysaccharides and antioxidants. Nine phenolics were identified predominantly with catechin (39.67 ± 1.5 µg/g). FM-fortification is reflected by enhancement in protein (49.5 ± 2.97 g/Kg) and fiber content (1.78 ± 0.54 g/Kg) compared to the FM content. Relative rats’ weight gain improved to 34.29% for the fortified-FM group close to the control; it was recorded at 16.47% for the AFM1 group. Alkaline phosphatase in AFM1 rats was 99.2 ± 1.86 U/L and decreased to 44.2 ± 0.71 U/L in the fortified-FM group (44.2 ± 0.71 U/L) to be close to the control group. Aflatoxin M1 rats exposure reflects tissue alterations and cell damage, which recorded lesser in rats treated by extract and beverage administrations. The beverage’s corrective action relied on two integrated mechanisms, aflatoxin-binding to bacterial and bioactivity interaction of extract substances. This beverage stopped tissue alterations that occurred due to aflatoxins. The result supports the future production of fortified-milk beverages as a bio-shield against aflatoxin toxicity, besides their nutritional and functional properties.
Do Ethnic-Racial Identity Dimensions Moderate the Relations of Outgroup Discrimination and Ingroup Marginalization to Self-esteem in Black and Latinx Undergraduates?
Authors: Wilson Antoinette R. , Leaper Campbell 
Published: December 2022

AbstractGuided by social identity and intergroup theory, we tested how two facets of ethnic-racial identity—felt typicality (perceived similarity to other ingroup members) and ingroup ties (felt closeness to other ingroup members)—potentially buffer the negative effects of outgroup discrimination and ingroup marginalization on self-esteem. Participants included 407 Latinx (65%) and Black (35%) undergraduates (Mage = 24.72 years, 79% women, 21% men) who completed an online survey. Our analyses yielded three key findings. First, both outgroup discrimination and ingroup marginalization predicted lower self-esteem; however, this association was significantly stronger with ingroup marginalization than outgroup discrimination. Second, the association between ingroup marginalization and self-esteem was reduced when ethnic-racial identity variables were controlled. Felt ethnic-racial typicality additionally moderated the association between ingroup marginalization and self-esteem—whereby the negative association was stronger when individuals felt higher ethnic-racial typicality. Our findings expand understanding of the impact of marginalization and discrimination from those within and outside of one’s ethnic-racial group, respectively. We also discuss the differing roles of ethnic-racial identity when experiencing outgroup discrimination and ingroup marginalization.
In memory of Professor Nenad Mladenović (1951–2022)
Authors: Sifaleras Angelo 
Published: December 2022

AbstractThis article is dedicated to the memory of Professor Nenad Mladenović. It provides a short biography, a description of his main scientific achievements, and also testimonials from scholars all over the world who were collaborators for several years. This peer recognition highlights not only his exceptional academic career but also the integrity of his character.
Molecular pattern of a decrease in the rewarding effect of cocaine after an escalating-dose drug regimen
Authors: Kołosowska Karolina , Lehner Małgorzata , Skórzewska Anna , Gawryluk Aleksandra , Tomczuk Filip , Sobolewska Alicja , Turzyńska Danuta , Liguz-Lęcznar Monika , Bednarska-Makaruk Małgorzata , Maciejak Piotr , Wisłowska-Stanek Aleksandra 
Published: December 2022

Abstract
Background
Long-term cocaine exposure leads to dysregulation of the reward system and initiates processes that ultimately weaken its rewarding effects. Here, we studied the influence of an escalating-dose cocaine regimen on drug-associated appetitive behavior after a withdrawal period, along with corresponding molecular changes in plasma and the prefrontal cortex (PFC).

Methods
We applied a 5 day escalating-dose cocaine regimen in rats. We assessed anxiety-like behavior at the beginning of the withdrawal period in the elevated plus maze (EPM) test. The reinforcement properties of cocaine were evaluated in the Conditioned Place Preference (CPP) test along with ultrasonic vocalization (USV) in the appetitive range in a drug-associated context. We assessed corticosterone, proopiomelanocortin (POMC), β-endorphin, CART 55–102 levels in plasma (by ELISA), along with mRNA levels for D2 dopaminergic receptor (D2R), κ-receptor (KOR), orexin 1 receptor (OX1R), CART 55–102, and potential markers of cocaine abuse: miRNA-124 and miRNA-137 levels in the PFC (by PCR).

Results
Rats subjected to the escalating-dose cocaine binge regimen spent less time in the cocaine-paired compartment, and presented a lower number of appetitive USV episodes. These changes were accompanied by a decrease in corticosterone and CART levels, an increase in POMC and β-endorphin levels in plasma, and an increase in the mRNA for D2R and miRNA-124 levels, but a decrease in the mRNA levels for KOR, OX1R, and CART 55–102 in the PFC.

Conclusions
The presented data reflect a part of a bigger picture of a multilevel interplay between neurotransmitter systems and neuromodulators underlying processes associated with cocaine abuse.
Hubbard U through polaronic defect states
Authors: Falletta Stefano , Pasquarello Alfredo 
Published: December 2022

AbstractSince the preliminary work of Anisimov and co-workers, the Hubbard corrected DFT+U functional has been used for predicting properties of correlated materials by applying on-site effective Coulomb interactions to specific orbitals. However, the determination of the Hubbard U parameter has remained under intense discussion despite the multitude of approaches proposed. Here, we define a selection criterion based on the use of polaronic defect states for the enforcement of the piecewise linearity of the total energy upon electron occupation. A good agreement with results from piecewise linear hybrid functionals is found for the electronic and structural properties of polarons, including the formation energies. The values of U determined in this way are found to give a robust description of the polaron energetics upon variation of the considered state. In particular, we also address a polaron hopping pathway, finding that the determined value of U leads to accurate energetics without requiring a configurational-dependent U. It is emphasized that the selection of U should be based on physical properties directly associated with the orbitals to which U is applied, rather than on more global properties such as band gaps and band widths. For comparison, we also determine U through a well-established linear-response scheme finding noticeably different values of U and consequently different formation energies. Possible origins of these discrepancies are discussed. As case studies, we consider the self-trapped electron in BiVO4, the self-trapped hole in MgO, the Li-trapped hole in MgO, and the Al-trapped hole in α-SiO2.
Protistan epibionts affect prey selectivity patterns and vulnerability to predation in a cyclopoid copepod
Authors: Kumar Ram , Kumari Suman , Malika Anshu , Sharma A. P. , Dahms Hans-Uwe 
Published: December 2022

AbstractColonisation of crustacean zooplankton with ciliate epibionts is widespread in freshwater and marine environments. However, the ecology of such association are little studied as yet. The occurrence of ciliate epibionts on copepods and the preference towards this association with different life stages of Mesocyclops were studied from winter to spring. Relative susceptibility of zooplankton species was evaluated by analysing the epibiont colonies and zooids and relate this to the surface area of the host. The maximum epibiont infestation per unit body surface area was recorded on copepodites followed by copepod nauplii rather than other zooplankton species, whereas the rotifer Asplanchna was never affected. Influence of climatic factors such as temperature on the colonisation of epibionts on basibionts was found significant. In winter (November to February) samples, copepods were infested by autotrophic epibionts whereas in late spring and early summer (March–April) heterotrophic protists (peritrichian ciliates) were the sole epibionts on copepods. We conducted experiments in the laboratory on prey selection pattern of predators by direct visual and video-graphic observations of various events (encounter, attack, capture, ingestion, prey escape) during predation by infested and uninfested copepodites and adults of Mesocyclops. Postencounter the attack probability was significantly lower in infested than in uninfested copepods. The present paper reports on substrate preference by epibionts and their impacts in food rich and food scarce environments. Furthermore, major environmental interactions were studied with the reproductive phenology of copepods with respect to epibionts and the cause and effect of long term association of epibionts with copepods need to be addressed.
Development of a high-performance open-source 3D bioprinter
Authors: Tashman Joshua W. , Shiwarski Daniel J. , Feinberg Adam W. 
Published: December 2022

AbstractThe application of 3D printing to biological research has provided the tissue engineering community with a method for organizing cells and biological materials into complex 3D structures. While many commercial bioprinting platforms exist, they are expensive, ranging from $5000 to over $1,000,000. This high cost of entry prevents many labs from incorporating 3D bioprinting into their research. Due to the open-source nature of desktop plastic 3D printers, an alternative option has been to convert low-cost plastic printers into bioprinters. Several open-source modifications have been described, but there remains a need for a user-friendly, step-by-step guide for converting a thermoplastic printer into a bioprinter using components with validated performance. Here we convert a low-cost 3D printer, the FlashForge Finder, into a bioprinter using our Replistruder 4 syringe pump and the Duet3D Duet 2 WiFi for total cost of less than $900. We demonstrate that the accuracy of the bioprinter’s travel is better than 35 µm in all three axes and quantify fidelity by printing square lattice collagen scaffolds with average errors less than 2%. We also show high fidelity reproduction of clinical-imaging data by printing a scaffold of a human ear using collagen bioink. Finally, to maximize accessibility and customizability, all components we have designed for the bioprinter conversion are provided as open-source 3D models, along with instructions for further modifying the bioprinter for additional use cases, resulting in a comprehensive guide for the bioprinting field.
Situation assessment in air combat considering incomplete frame of discernment in the generalized evidence theory
Authors: Zhou Ying , Tang Yongchuan , Zhao Xiaozhe 
Published: December 2022

AbstractFor situation assessment in air combat, there may be incomplete information because of new technologies and unknown or uncertain targets and threats. In this paper, an improved method of situation assessment for air combat environment considering incomplete frame of discernment in the evidence theory is proposed to get a more accurate fusion result for decision making in the battlefield environment. First, the situation in air combat is assessed with knowledge. Then, the incomplete frame of discernment in the generalized evidence theory, which is an extension of Dempster–Shafer evidence theory, is adopted to model the incomplete and unknown situation assessment. After that, the generalized combination rule in the generalized evidence theory is adopted for fusion of situations in intelligent air combat. Finally, real-time decision-making in situation assessment can be reached for actions to take. Experiments in situation assessment of air combat with incomplete and uncertain situations show the rationality and effectiveness of the proposed method.
Significance of thermal radiation and bioconvection for Williamson nanofluid transportation owing to cone rotation
Authors: Abdal Sohaib , Siddique Imran , Eldin Sayed M. , Bilal Muhammad , Hussain Sajjad 
Published: December 2022

AbstractNumerical investigation for enhancement in thermal distribution of unsteady dynamics of Williamson nanofluids and ordinary nanofluids flow across extending surface of a rotating cone is represented in this communication. Bio-convection of gyrotactic micro-organisms and thermal radiative fluxes with magnetic fields are significant physical aspects of the study. The velocity slip conditions are considered along x and y directions. The leading formulation is transmuted into ordinary differential form via similarity functions. Five coupled equations with non-linear terms are resolved numerically through the utilization of Matlab code for the Runge–Kutta procedure. The parameters of buoyancy ratio and bio-convection Rayleigh number decrease the x-direction velocity. The slip parameter being proportional to viscosity reduces the speed of flow and hence rise in temperature. Also, the temperature rises with the rising values of magnetic field strength, radiative heat transportation, Brownian motion and thermophorsis.
miRNA expression profiles of the perilesional skin of atopic dermatitis and psoriasis patients are highly similar
Authors: Carreras-Badosa Gemma , Maslovskaja Julia , Vaher Helen , Pajusaar Laura , Annilo Tarmo , Lättekivi Freddy , Hübenthal Matthias , Rodriguez Elke , Weidinger Stephan , Kingo Külli , Rebane Ana 
Published: December 2022

AbstractAtopic dermatitis (AD) and psoriasis vulgaris (PV) are chronic inflammatory skin diseases with heterogeneous molecular backgrounds. MicroRNAs (miRNAs) contribute to either development or regulation of many immune system related diseases. Only few miRNA profiling studies are available for AD and no comparisons between AD and PV skin miRNA profiles have been performed recently. We conducted a miRNA profiling analysis of skin, as well as serum, from adult AD and PV patients and control individuals. 130 miRNAs were differentially expressed in AD skin, of which 77 were common differentially expressed in AD and PV. No differentially expressed miRNAs were detected in serum. Pathway analyses revealed differentially expressed miRNAs to potentially target immune-system related pathways, including TNF-α, IL-2/STAT4 and IL-6/JAK/STAT3. Additional genetic analysis of published AD GWAS dataset detected association of several target genes of differentially expressed miRNAs in skin. Moreover, miR-28-5p, miR-31-5p, miR-378a-3p and miR-203a were validated as upregulated in the skin of AD and PV patients. All validated miRNAs were reliable predictive markers for AD or PV. In conclusion, miRNA expression pattern in the skin of adult AD patients is highly similar to that of PV with multiple differentially expressed miRNAs potentially involved in the regulation of immune responses in AD and PV.
Chromatin remodeler CHD7 targets active enhancer region to regulate cell type-specific gene expression in human neural crest cells
Authors: Sanosaka Tsukasa , Okuno Hironobu , Mizota Noriko , Andoh-Noda Tomoko , Sato Miki , Tomooka Ryo , Banno Satoe , Kohyama Jun , Okano Hideyuki 
Published: December 2022

AbstractA mutation in the chromatin remodeler chromodomain helicase DNA-binding 7 (CHD7) gene causes the multiple congenital anomaly CHARGE syndrome. The craniofacial anomalies observed in CHARGE syndrome are caused by dysfunctions of neural crest cells (NCCs), which originate from the neural tube. However, the mechanism by which CHD7 regulates the function of human NCCs (hNCCs) remains unclear. We aimed to characterize the cis-regulatory elements governed by CHD7 in hNCCs by analyzing genome-wide ChIP-Seq data and identifying hNCC-specific CHD7-binding profiles. We compared CHD7-binding regions among cell types, including human induced pluripotent stem cells and human neuroepithelial cells, to determine the comprehensive properties of CHD7-binding in hNCCs. Importantly, analysis of the hNCC-specific CHD7-bound region revealed transcription factor AP-2α as a potential co-factor facilitating the cell type-specific transcriptional program in hNCCs. CHD7 was strongly associated with active enhancer regions, permitting the expression of hNCC-specific genes to sustain the function of hNCCs. Our findings reveal the regulatory mechanisms of CHD7 in hNCCs, thus providing additional information regarding the transcriptional programs in hNCCs.
Live probiotic bacteria administered in a pathomimetic Leaky Gut Chip ameliorate impaired epithelial barrier and mucosal inflammation
Authors: Min Soyoun , Than Nam , Shin Yong Cheol , Hu Grace , Shin Woojung , Ambrosini Yoko M. , Kim Hyun Jung 
Published: December 2022

AbstractHere, we report a pathomimetic Leaky Gut Chip that recapitulates increased epithelial permeability and intestinal inflammation to assess probiotic intervention as live biotherapeutics. We leveraged a mechanodynamic human gut-on-a-chip (Gut Chip) that recreates three-dimensional epithelial layers in a controlled oxygen gradient and biomechanical cues, where the addition of a cocktail of pro-inflammatory cytokines, TNF-α and IL-1β, reproducibly induced impaired epithelial barrier followed by intestinal inflammation. This inflamed leaky epithelium was not recovered for up to 3 days, although the cytokine treatment ceased. However, when probiotic bacteria, either Lactobacillus rhamnosus GG or a multi-species mixture (VSL#3), were respectively administered on the leaky epithelium, bacterial cells colonized mucosal surface and significantly improved barrier function, enhanced the localization of tight junction proteins such as ZO-1 and occludin, and elevated mucus production. In addition, inflammatory markers, including p65, pSTAT3, and MYD88, that were highly expressed in the germ-free control were significantly reduced when probiotic bacteria were co-cultured in a Leaky Gut Chip. Probiotic treatment also significantly reduced the production of secretory pro-inflammatory cytokines. Hence, our pathomimetic Leaky Gut Chip may offer a translational strategy to dissect the therapeutic mechanism of live biotherapeutic products and validate their clinical potential by incorporating patient-derived organoids.
Line impedance compensation control strategy for multiple interlinking converters in hybrid AC/DC microgrid
Authors: Wang Can , Deng Can , Pan Xuewei 
Published: December 2022

AbstractIn a hybrid AC/DC microgrid (MG), the interlinking converter (IC) plays an important role in power flow regulation between subgrids. However, line impedance will cause deviations in the power transmitted by the IC if a communication device is not used. To realize accurate power delivery between subgrids without communication, an IC control method based on line impedance compensation is proposed. A harmonic signal is injected into the IC in this method using converters of distributed generation, which are connected to an AC bus. The specified frequency of the signal indicating the voltage of the AC bus can be acquired by an IC using a filter and phase‐locked‐loop (PLL) so that the IC can obtain line impedance by local information and then compensate for voltage drop to realize accurate power transmission. The entire implementation of this method uses no communication. Moreover, when multiple ICs run in parallel, this method can also be conducted easily and effectively to realize accurate power flow between subgrids and power sharing among ICs in proportion to the ICs’ rated capacity. This strategy is verified by simulation and hardware‐in‐the‐loop tests.
Electrical treeing behaviours in cross‐linked polyethylene cables after thermal ageing
Authors: Zhang Yi , Wu Zaijun , He Jiahong , Zhang Dongdong , Hu Minqiang 
Published: December 2022

AbstractLong‐term thermal ageing might have impacts on the physicochemical properties of cross‐linked polyethene (XLPE) cables, thereby influencing their electrical behaviours, such as electrical treeing initiation and growth. The main objective of this work is to report the effects of thermal ageing on the electrical treeing behaviours (initiation and growth) in XLPE cable insulation. For this purpose, the electrical treeing initiation and growth test were performed in accelerated thermal samples. In addition, Differential Scanning Calorimetry and Fourier Transform Infrared Spectroscopy were utilised to investigate corresponding changes in the microstructures of materials. In addition, a correlation was established between electrical treeing behaviours and physicochemical properties during the thermal ageing process. Test results indicate that the thermal ageing process should be divided into three phases, and the re‐crystallisation reaction is considered the primary reason for the increase of average electrical tree initiation voltage (AETIV). Furthermore, the thermal ageing index Xc is correlated to AETIV and the propagation rate of the electrical tree. As equivalent relationships have been established between the relative decrease value w of indexes AETIV, the propagating rate and Xc, this method can be employed to predict the changing pattern of electrical treeing initiation voltage and the propagating rate in future research.