bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and clinical significance of Ki-67 and P16 after neoadjuvant chemotherapy for locally advanced cervical cancer
Authors: Wu Jialin , Wang Rong , Chen Wanli , Wu Yingyu , Xiao Lin 
Published: March 2023

Abstract
Objective
To investigate the relationship between Ki-67 and P16 expression levels after neoadjuvant chemotherapy and the clinicopathological characteristics and prognosis of patients with locally advanced cervical cancer.
Methods
Patients who underwent neoadjuvant chemotherapy combined with radical hysterectomy in stage IB2 and IIA2 (FIGO 2009) at the First Hospital of Chongqing Medical University between January 2015 and December 2019 were retrospectively collected to correlate postoperative ki-67 and P16 expression levels with clinicopathological factors, and the optimal threshold for predicting recurrence was analyzed using ROC curves for the Ki67 index and univariate and multifactorial Cox regression analysis for the association between clinicopathological features including ki-67 and P16 and recurrence-free survival of the disease.
Results
A total of 334 patients had a median Ki-67 index of 60% (0–90%), with 303 (90.7%) positive and 31 (9.3%) negative P16 expressions; The cut-off value of ki-67 for determining patient recurrence was 67.5% according to the Receiver operating characteristic curves (ROC) (ROC curve) analysis; in multifactorial Cox analysis, lymphatic vascular space (p = 0.003) and ki-67 index (p = 0.005) increased the risk of tumor recurrence and were independent prognostic factors for cervical cancer recurrence, while the expression of P16 levels was not statistically significant (p = 0.097 OR = 0.319), and patients with cervical cancer in the high Ki-67 expression group (Ki-67 ≥ 67.5%) had lower recurrence-free survival (RFS) and overall survival (OS) than those in the low Ki-67 expression group (Ki-67 < 67.5%) (p = 0.001, p = 0.036).
Conclusion
The expression levels of ki-67 and P16 after neoadjuvant chemotherapy for locally advanced cervical cancer correlated with tumor differentiation; ki-67 values of 67.5% predicted the risk of recurrence in patients with stage IB2 and IIA2. High expression of ki-67 (Ki-67 ≥ 67.5%) may indicate poorer recurrence-free survival and overall survival, positive expression of P16 was not significantly associated with the prognosis of patients with stage IB2 and IIA2 cervical cancer.
Integration of microbattery with thin-film electronics for constructing an integrated and transparent microsystem based on InGaZnO
Authors: Jia Bin , Zhang Chao , Liu Min , Li Zhen , Wang Jian , Zhong Li , Han Chuan Yu , Qin Ming , Huang Xiaodong 
Published: March 2023

Abstract
Transparent electronics hold promising applications in emerging consumer electronics. A full integration of miniaturized transparent energy device (lithium-ion battery, LIB), electronic device (thin-film transistor, TFT)) and sensing device (photodetector, PD) to form a monolithic integrated microsystem greatly enhances the functions of transparent electronics. However, an integrated transparent microsystem including all the above-mentioned devices has not been realized. In this work, InGaZnO (IGZO) is explored to prepare the above devices and microsystem due to its multifunctional and outstanding properties. A transparent LIB with IGZO as the anode is first proposed as the on-chip power source. Then, TFT with IGZO as the channel and PD with IGZO as the photosensitive are also prepared on the same substrate for constructing an integrated transparent microsystem. Each device displays acceptable performance, including that: LIB shows a specific capacity of 9.8 µAh cm-2; TFT displays a carrier mobility of 3.3 cm2 V-1 s-1; PD shows a responsivity of 0.35 A W-1. Moreover, AC signals can be successfully charged into LIB by using TFT as the on-chip rectifier and also the PD works well by using the charged LIB as the on-chip power, demonstrating collaborative capabilities of each device to achieve systematical function.
Anemia prevalence and its predictors among children under-five years in Ghana. A multilevel analysis of the 2019 Ghana malaria indicator survey
Authors: Aheto Justice Moses K. , Alhassan Yakubu , Puplampu Adikwor Ewoenam , Boglo Julius Kuenyegbe , Sedzro Kojo Mensah 
Published: March 2023

Abstract
Despite the implementation of anemia control interventions in Ghana, prevalence of anemia is still high in children under-five years although it has reduced by 24% between 2008 and 2019. Further reducing the prevalence of anemia requires identifying and targeting critical risk factors associated to anemia. This study seeks to identify predictors of anemia among children under-five years in Ghana. Data from 2019 Ghana MIS was analyzed using both fixed and random effect logistic regression models. Among the 2434 children under-five years, 54% (95% CI 52.0, 57.0) of them were anemic. In the multivariable multilevel model, the risk of anemia was found to be higher in younger children, children who had malaria, children whose mothers were not covered for health insurance, adolescent mothers’, non-Christian mothers and poorer households. Significant unobserved community level effects in anemia prevalence were observed. We observed high prevalence of anemia in children under-five and critical risk factors at the child, household, and community levels in this population of children. Multifaceted and targeted approaches are needed to help reduce the anemia prevalence in this setting so as to achieve multiple SDGs which are related to risk and prevalence of anemia by 2030.
Improved equality of human exposure to greenspace in the 21st century urbanization
Authors: Wu Shengbiao , Chen Bin , Webster Chris , Xu Bing , Gong Peng 
Published: March 2023

Abstract
Greenspace plays a crucial role in urban ecosystems and has been recognized as a key factor in promoting sustainable and healthy city development. Recent studies have revealed a growing concern about urban greenspace exposure inequality; however, the extent to which urbanization affects human exposure to greenspace and associated inequalities over time remains unclear. Here, we incorporate a Landsat-based 30-meter time-series greenspace mapping and a population-weighted exposure framework to quantify the changes in human exposure to greenspace for 1028 global cities between 2000–2018. Results show a substantial increase in physical greenspace coverage and an improvement in human exposure to urban greenspace, leading to a reduction in greenspace exposure inequality over the past two decades. Nevertheless, we observe a contrasting difference in the rate of reduction in greenspace exposure inequality between cities in the Global South and North, with a faster rate of reduction in the Global South, nearly four times that of the Global North. These findings provide valuable insights into the impact of urbanization on urban nature and environmental inequality change and can inform future city greening efforts.
Thermomechanical Response of Additively Manufactured Inconel 718 during Hot Torsion Tests
Authors: Yadav pinku , Rigo Olivier , Arvieu Corinne , Lacoste Eric 
Published: March 2023

Abstract
In this study, we have investigated the microstructural changes during deformation of the SLM samples printed Inconel 718 using hot torsion tests. Prior to thermomechanical testing, the as-built samples are homogenized at 850°C for 2 h in Ar atmosphere followed by air cooling. Hot torsion tests are performed at temperatures of 850, 1000 and 1100°C, and at strain rates of 0.01, 0.1 and 1 s− 1. It is observed that the dynamic recrystallization mechanism progresses with the evolution of annealing twins which is quantified by the increase in % of annealing twins during thermomechanical deformation. It is observed that samples deformed at 850°C for strain rates of 0.01 and 0.1 s− 1 shows brittle like fracture and no dynamic recrystallization. However, the samples deformed at 1000 and 1100°C for strain rates of 0.01, 0.1 and 1 s− 1 shows typical stress strain curves obtained for dynamic recrystallization. It is noted that the peak stress decreases with an increase in the temperature and increases with an increase in the strain rate. Alongside, an activation energy (Q) of 379.62 KJ/mol for SLMed Inconel 718 samples is calculated which agrees with the reported values in the literature for Hot compression tests.
The Planetary Child Health and Enterics Observatory (Plan-EO): a Protocol for an Interdisciplinary Research Initiative and Web-Based Dashboard for Mapping Enteric Infectious Diseases and their Risk Factors and Interventions in Low- and Middle-Income Countries
Authors: Colston Josh M. , Chernyavskiy Pavel , Gardner Lauren , Fang Bin , Houpt Eric , Swarup Samarth , Badr Hamada , Zaitchik Benjamin , Lakshmi Venkataraman , Kosek Margaret 
Published: March 2023

Abstract

Background: Diarrhea remains a leading cause of childhood illness throughout the world and is caused by various species of ecologically sensitive pathogens. The emerging Planetary Health movement emphasizes the interdependence of human health with natural systems, and much of its focus has been on infectious diseases and their interactions with environmental and human processes. Meanwhile, the era of big data has engendered a public appetite for interactive web-based dashboards for infectious diseases. However, enteric infectious diseases have been largely overlooked by these developments.
Methods: The Planetary Child Health and Enterics Observatory (Plan-EO) is a new initiative that builds on existing partnerships between epidemiologists, climatologists, bioinformaticians, and hydrologists as well as investigators in numerous low- and middle-income countries. Its objective is to provide the research and stakeholder community with an evidence base for the geographical targeting of enteropathogen-specific child health interventions such as novel vaccines. The initiative will produce, curate, and disseminate spatial data products relating to the distribution of enteric pathogens and their environmental and sociodemographic determinants.
Discussion: To date Plan-EO has compiled data from 23 studies comprising almost 80,000 stool samples from 35,000 children aged 0 – 59 months at 80 sites in 24 countries and georeferenced to over 9,000 unique locations, with DUAs for two further studies under negotiation. An initial analysis of Shigella has been published and has yielded detailed prediction maps.Results like these can be used to identify and target priority populations living in transmission hotspots and to provide an urgently needed evidence base for decision-making, scenario-planning.
Study registration: PROSPERO protocol #CRD42023384709
Developing novel indanone-chalcone hybrids bearing ester group with potential anti-Alzheimer effects by combining molecular hybridization and bioisosteric replacement drug design approaches
Authors: Azimian Fereshteh , Shahrivar-Gargari Mohammad , vahedpour Teymour , Hemmati Salar , Dastmalchi Mahrokh , Küçükkılınç Tuba Tüylü , Dastmalchi Siavoush 
Published: March 2023

Abstract
Molecular hybridization and bioisosteric replacement approaches were used to design novel agents with potential use for the treatment of Alzheimer’s disease (AD). Derivatives containing indanone-chalcone core structure bearing ester group were designed and synthesized. In vitro activities of all target compounds against acetylcholinesterase (AChE) were evaluated. The most active compound 8e exhibited anticholinesterase inhibitory activity with IC50 value of 18.7 µM against AChE while the reference drug donepezil (IC50 = 0.0.36 µM on AChE) was used as a control. Also, Aβ1–40 aggregation inhibitory activities of the synthesized compounds were evaluated, and the results showed that compound 8h demonstrates the highest Aβ1–40 aggregation inhibition (81.6%). The results of docking studies revealed that some of compounds fits well into the binding site of AChE. Collectively, this study provide insight for design of small molecules including ester groups to target acetyl choline esterase for further study in the process of design and development of active compounds for AD.
Mitogen activated protein kinase phosphatase 5 alleviates liver ischemia-reperfusion injury by inhibiting TAK1/JNK/p38 pathway
Authors: Yu Qiwen , Chen Sanyang , Li Jiye , Tang Hongwei , Shi Jihua , Guo Wenzhi , Zhang Shuijun 
Published: March 2023

Abstract
MKP5 is a member of the MKP family and has been implicated in diverse biological and pathological conditions. However, it is unknown what role MKP5 plays in liver ischemia/reperfusion (I/R) injury. This study aimed to investigate the role of MKP5 in liver I/R injury. In the present study, we used MKP5 global knockout (KO) and MKP5 overexpressing mice to establish a liver I/R injury model in vivo, and we used MKP5 knockdown or MKP5 overexpressing HepG2 cells to establish a hypoxia-reoxygenation (H/R) model in vitro. In this study we demonstrated that MKP5 expression was significantly down-regulated in liver tissue of mice after liver I/R injury and HepG2 cells subjected to H/R insult. MKP5 knockdown significantly increased liver injury, as demonstrated by elevated serum transaminases, hepatocyte necrosis, infiltrating inflammatory cells, secretion of pro-inflammatory cytokines, apoptosis, ROS production, MDA contents, decreased cellular viability, SOD and GSH activities in vitro and in vivo. Conversely, MKP5 overexpression significantly attenuated liver and cell injury in our models. Furthermore, we showed that MKP5 exerted its protective effect by inhibiting c-Jun N-terminal kinase (JNK)/p38 mitogen-activated protein kinase (p38) activity, and its action was dependent on Transforming growth factor-β-activated kinase 1 (TAK1) activity. In addition, inhibition of TAK1 remarkably abolished the damaging effect result from MKP5 knockdown in cell H/R injury in vitro. According to our results, MKP5 inhibited the TAK1/JNK/p38 pathway to protect liver from injury induced by I/R. Our study identifies a novel target for liver I/R injury diagnosis and treatment.
DNA methylation in the wild: epigenetic transgenerational inheritance can mediate adaptation in clones of wild strawberry (Fragaria vesca)
Authors: Sammarco Iris , Rodríguez Bárbara Díez , Galanti Dario , Nunn Adam , Becker Claude , Bossdorf Oliver , Münzbergová Zuzana , Latzel Vít 
Published: March 2023

Abstract
Due to the accelerating climate change, it is crucial to understand how plants adapt to rapid environmental changes. Such adaptation may be mediated by epigenetic mechanisms like DNA methylation, which could heritably alter phenotypes without changing the DNA sequence, especially across clonal generations. However, we are still missing robust evidence of the adaptive potential of DNA methylation in wild clonal populations.
Here, we studied the methylome of Fragaria vesca, a predominantly clonally reproducing herb. We examined samples from 21 natural populations across three climatically distinct geographic regions, as well as clones of the same individuals grown in a common garden. We found that inherited epigenetic variation was partly associated with climate of origin, and that a subset of these epigenetic changes affected gene expression. Our results indicate that DNA methylation variation in the wild is common and may contribute to adaptation of clonal plant populations.
Evolution mechanism of diagenesis of deep sag area in the central Fault basin—A case study of the Wuerhe Formation of the Dongdaohaizi sag in the Junggar basin, Northwest China
Authors: Wang Jinkai , Wu Rui , Xie Jun 
Published: March 2023

Abstract
The Junggar basin is a typical terrestrial fault basin. The stratum in the central area is deeply buried and its sedimentary and rock types are diverse. Its diagenesis is complex and the evolution law of reservoir pores is challenging to characterize, which is not conducive to oil and gas exploration in this region. In this paper, the Dongdaohaizi sag in the central deep basin was taken as an example to be detailed on its sedimentary facies, diagenesis, and micropore structure, revealing the diagenesis sequence and pore evolution law of the deep buried reservoir. First, the sedimentary facies type of the focus stratum (Wuerhe Formation) is assigned to the fan delta, its source is from the northwest, and the rock type is conglomerate, pebbly sandstone, sandstone, and siltstone. The debris content is high among the clastic grains and can reach 40%, showing near-source transport characteristics. Second, the primary diagenesis types and evolution sequence of the Wuerhe Formation is cleared according to experiment data, and the influence degree of the compassion, cementation, and dissolution of the target reservoir rock is clarified. Finally, the sandstone pore evolution model is established, the diagenesis facies is classified, the contribution degree of the unstable clasts and cemented minerals to the secondary pore is determined, and the comprehensive factors with the greatest impact on the late densification of the reservoir are clarified. The diagenesis and pore evolution of the deep rocks in the deep central sag are revealed.
Decision support system tool of the “Electronic-Implementation of Maternal Death/Mortality Review (E-MPATHY)” for formulating evidence-based recommendations with the learning environment at the district level: Development, Validation, Reliability, and Acceptability
Authors: Cahyanti Ratnasari D. , Widyawati Widyawati , Hakimi Mohammad 
Published: March 2023

Abstract

Background: Maternal Death Review (MDR), the cornerstone of Maternal Death Surveillance and Response (MDSR), provides evidence-based recommendations for reducing maternal death. A key difficulty in conducting MDR is the existence of a blaming culture leading to inaccuracy of maternal death classification and delays for policy-making. To support the conducive learning environment at local levels, scientific evidence and clinical standards as the main structure in MDR are required. However, an integrated decision support system tool is absent. This study reports the development, validation, reliability, and acceptability of a decision support system tool to formulate evidence-based recommendations for a supportive learning environment of MDR at district levels.
Methods: Tool development comprised four steps: needs assessment, face and content validity, reliability, and acceptability assessment. The kappa (κ) value was calculated to assess inter-observer reliability in examining 113 maternal mortalities by the district and province review teams in Central Java, Indonesia. Reviewers’ acceptability was identified with observations during the review and analysis applying the Theoretical Framework of Acceptability.
Results: The needs assessment resulted in: 1) naming the tool as the “Electronic Implementation of Maternal Death/Mortality Review” (E-MPATHY) reflecting a supportive learning environment; 2) a conceptual structure of a solution-based approach in each pregnancy period with integrated scientific evidence and the 3-delays model as a flow framework to assign the underlying cause based on the International Classification of Diseases-Maternal Mortality (ICD-MM), the contributing events, and recommendations. The E-MPATHY was found to be valid (CVI=1) and readable. An almost perfect agreement was found between the causes of maternal death assigned by the district and province reviewers using the ICD-MM, κ=0.86 (95%CI:0.76-0.96); p<0.001. In the 3-delays assessment, the highest agreement was found in the postpartum period (κ=0.58, 95%CI:0.42-0.74; p<0.001) with a substantial value (κ=0.61, 95%CI:0.46-0.75; p<0.001) in the third phase delay. The reviewer’s acceptability highlighted the benefits of reviewing using scientific evidence and providing encouragement for positive attitudes toward learning in their daily practices.
Conclusion: The E-MPATHY performs as a useful tool to guide formulating evidence-based recommendations in a standardized way. The tool can support direct learning and be potentially applied to institutionalizedworkable district MDR.
In vitro studies of factors affecting debridement of dental implants by tricalcium phosphate powder abrasive treatment
Authors: Chen I-Cheng , Su Chen-Ying , Tu Jia-Jun , Kao Daniel Wenkai , Fang Hsu-Wei 
Published: March 2023

Abstract
Peri-implantitis is a common complication characterized by inflammation in tissues with plaque accumulation around dental implants, causing dental implantation failures. Among current managements, air flow abrasive treatment was found to be efficient for the debridement of implant surfaces, however, little information is understood about factors affecting cleaning capacity. In particular, this study examined the cleaning capacity of air powder abrasive (APA) treatment with β-tricalcium phosphate (β-TCP) powder systematically by utilizing various powder jetting strength and different particle sizes. β-TCP powder was prepared in three sizes (S, M and L) and strength of different powder setting (low, medium, high) was estimated. The cleaning capacity was determined by quantifying the ink removal simulating biofilm removal from the implant surfaces. In the light of these systematical comparisons, it can be concluded that complete implant surface cleaning could be achieved by higher jetting strength of APA treatment with smaller β-TCP particles. Moreover, the amount of consumed powder seemed critical to the cleaning efficiency. Implant surfaces and sandblast and acid etching (SLA) structure of titanium discs were altered in all tested groups. The systematically analyzed outcomes in this study might provide an insight into the development of potential strategies to treat peri-implant diseases.
The intrapartum strategy for thoracoscopic repair of congenital diaphragmatic hernia(CDH): five cases report and literature review
Authors: Yu Leiyuan , Sun Yina , Chen Jiamin , Yang Mengmeng , Jiang Ying , Dong Tian , Zhao Baihui , Lv Changcheng , Tan Zheng , Chen Yuan , Luo Qiong 
Published: March 2023

Abstract
Purpose: To introduce a new method of minimally invasive repair of Congenital diaphragmatic hernia intrapartum(CDH).
Methods: We present five CDH cases diagnosed prenatally. Each patient was evaluated by a multidisciplinary team and received thoracoscopic defect repair on placental support. The neonates were transferred to NICU for further treatment, and all cases were under follow-up.
Results: The patients enrolled were mild to moderate, with thoracic herniation of the liver occurring in one case. All five cases were repaired on placenta support. Maternal morbidity was reported in one case for acute pulmonary embolism (case 2), but no recorded case of maternal mortality. Postoperative neonatal death occurred in two cases (case 2,3), the remaining three neonates are under follow-up with a good prognosis.
Conclusion: The intrapartum thoracoscopic repair is feasible and safe for selected CDH cases.