bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phospholipid scramblase 3: a latent mediator connecting mitochondria and heavy metal apoptosis
Authors: Palanirajan Santosh Kumar , Gummadi Sathyanarayana N 
Published: March 2023

Abstract
Lead and mercury are the ubiquitous heavy metals triggering toxicity and initiating apoptosis in cells. Though the toxic effects of heavy metals on various organs are known, there is a paucity of information on the mechanisms that instigate the current study. A plausible role of phospholipid scramblase 3 (PLSCR3) in Pb2+ and Hg2+ induced apoptosis was investigated with human embryonic kidney (HEK 293) cells. After 12 h of exposure, ~30-40% of the cells were in the early stage of apoptosis with increased reactive oxygen species (ROS), decreased mitochondrial membrane potential, and increased intracellular calcium levels. Also, ~20% of the cardiolipin localized within the inner mitochondrial membrane was translocated to the outer mitochondrial membrane along with the mobilization of truncated Bid (t-Bid) to the mitochondria and cytochrome c from the mitochondria. The endogenous expression levels of PLSCR3, caspase 8, and caspase 3 were upregulated in Pb2+ and Hg2+ induced apoptosis. The activation and upregulation of PLSCR3 mediate CL translocation playing a potential role in initiating the heavy metal-induced apoptosis. Therefore, PLSCR3 could be the linker between mitochondria and heavy metal apoptosis.
MCM4 expression is associated with high-grade histology, tumor progression and poor prognosis in urothelial carcinoma
Authors: Kobayashi Go , Hayashi Tetsutaro , Sentani Kazuhiro , Uraoka Naohiro , Fukui Takashi , Kido Aya , Katsuya Narutaka , Ishikawa Akira , Babasaki Takashi , Sekino Yohei , Nose Hiroyuki , Arihiro Koji , Hinata Nobuyuki , Oue Naohide 
Published: March 2023

Abstract
Background
We previously reported Minichromosome maintenance 4 (MCM4) overexpression in gastric cancer. However, the clinicopathological significance of MCM4 in urothelial carcinoma (UC) has not been investigated. To clarify the clinicopathological significance of MCM4 in UC, we investigated MCM4 expression with immunohistochemistry (IHC).
Methods
We analyzed the expression and distribution of MCM4 in 124 upper tract urothelial carcinoma (UTUC) samples by immunohistochemistry. Additionally, using 108 urine samples, we analyzed MCM4 expression in urine cytology.
Results
In normal urothelium, MCM4 expression was weak or absent. Meanwhile, the strong nuclear expression of MCM4 was observed in UTUC tissues, and it was detected in 77 (62%) of a total of 124 UTUC cases. MCM4-positive UTUC cases were associated with nodular/flat morphology, high grade, high T stage, and poor prognosis. Moreover, MCM4 expression was significantly higher in the invasive front than in the tumor surface. Similar results were also obtained in TCGA bladder cancer cohort. Additionally, MCM4 expression was associated with highly expression of Ki-67, HER2, EGFR, and p53 in UTUC. Among representative cancer-related molecules, MCM4 had an independent predictive value for progression-free survival and high-grade UC. Immunocytochemistry (ICC) for MCM4 was also performed on urine cytology slides and showed that the nuclear expression of MCM4 was more frequently found in UC cells than in non-neoplastic cells. The diagnostic accuracy of urine cytology was improved by combining MCM4 immunostaining with cytology.
Conclusion
These results suggest that MCM4 might be a useful predictive biomarker for high-grade histology, tumor progression and poor prognosis in UC. Moreover, ICC for MCM4 might be helpful for UC detection as additional markers in the cytomorphology-based diagnosis.
Efficacy and Safety of Phaseolus angularis L for. as Anti- aging Therapy in Healthy Skin
Authors: Oh Sarang , Jeong Jeehaeng , Jin Xiangji , Kim Myeongju , Zheng Shengdao , Kim Yong-Min , Yi Tae-Hoo 
Published: March 2023

Abstract
Skin photoaging is caused by prolonged exposure to ultraviolet (UV) radiation from sunlight. UV-exposed skin appears rough, thick, dry, loose, and showsdeep wrinkles, and thickening. Our previous research showed that a Phaseolus angularis seed extract (PASE) effectively inhibits photoaging through UVB protection in HaCaT cells. However, its efficacy has not been evaluated in clinical trials so far. In this single-centered, randomized, double-blind study, we investigated the anti-aging effect of PASE in eye wrinkle areas. To these 21 healthy adult women aged 30 to 59, a PASE cream was applied to the right eye wrinkle area and a placebo to the left eye wrinkle area twice a day (morning and evening) for 12 weeks. The change in thick, deep crease wrinkles around the eyes was confirmed by visual evaluation, skin measurements, and a questionnaire. As a result, the R1 (surface roughness), R2 (maximum roughness), R3 (average roughness), R4 (smoothness depth), and R5 (arithmetic mean roughness) values in the group using the PASE cream all decreased. Particularly, R1, R4, and R5 significantly decreased by 18.1%, 18.6%, and 25.0%. In addition, no participants reported side effects. Our study showed that PASE led to clinically significant levels of wrinkle improvement. In conclusion, as PASE is a natural food, safe, and with few side effects, it can be a good resource for natural anti-wrinkle cosmetics in the future.
Global existence and exponential stability of a dynamic frictionless contact problem with infinite memory and adhesion
Authors: Ouakil Imane , Benabderrahmane Benyattou , Boukhatem Yamna 
Published: March 2023

Abstract
In this paper, we study a dynamic contact problem between a nonlinear viscoelastic body and a foundation. The contact is adhesive and frictionless. The adhesion is modelled with a surface internal variable β. The contact is described with a version of a normal compliance condition. We prove the global existence of the solution. The proof is based on arguments of semigroup theory under suitable hypothesis on initial data and kernel memory function. Moreover, we obtain the exponential stability of the solution by using energy method to produce a suitable Lyapunov function.
Mathematics Subject Classification (2000). 74M15; 35B35; 74G25; 47H20.
An efficient vision-based parking slot detection method
Authors: Song Shaojing , Liu Zaiqing , Tang Pan , Miao Zhiqing , Hu Jinyan 
Published: March 2023

Abstract

High-accuracy detection of vacant parking slots using visual system is a prerequisite for automatic parking, which is significant for the promotion of automatic parking. Complex visual environment such as light occlusion, intensity variations and visual accuracy raises significant challenges for vision-based system parking slot detection. To address such problems, REPSDet (Resnet50 based Parking Slot Detection) is proposed for parking slot detection and occupancy classification in this paper, which is based on deep learning. Parking slot proximity information could be fully extracted by REPSDet,which is combined with parking slot classification and location information to improve the parking slot detection effect. For parking slot detection, REPS-Net is proposed in this paper, which is an improved yolov4 parking slot detection method. In REPS-Net, marker point positioning combined with geometric clues are used to infer the precise location of parking slots. For occupancy classification, the deep convolutional network m\_DCNN is proposed in this paper. Network structure and operation performance are optimized by m_DCNN, and the accuracy of parking slot detection is further improved. Experimental results show that REPSDet achieves precision rate of 97.77% and recall rate of 99.38% on the PS2.0_A dataset, which is significantly better than compared methods.
Dual-comb optomechanical spectroscopy
Authors: Ren Xinyi , Pan Jin , Yan Ming , Sheng Jiteng , Yang Cheng , Zhang Qiankun , Ma Hui , Wen Zhaoyang , Huang Kun , Wu Haibin , Zeng Heping 
Published: March 2023

Abstract
Optical cavities are essential for enhancing the sensitivity of molecular absorption spectroscopy, which finds widespread high-sensitivity gas sensing applications. However, the use of high-finesse cavities confines the wavelength range of operation and prevents broader applications. Here, we take a different approach to ultrasensitive molecular spectroscopy, namely dual-comb optomechanical spectroscopy (DCOS), by integrating the high-resolution multiplexing capabilities of dual-comb spectroscopy with cavity optomechanics through photoacoustic coupling. By exciting the molecules photoacoustically with dual-frequency combs and sensing the molecular-vibration-induced ultrasound waves with a cavity-coupled mechanical resonator, we obtain high-resolution broadband (>2 THz) overtone spectra for acetylene gas. The detection limit down to 15 parts per trillion is achieved without the restriction on the comb wavelengths. Our innovative approach not only enriches the practical applications of the emerging cavity optomechanics technology but also offers intriguing possibilities for multi-species trace gas detection.
The D-A-R approach: a method for determining ecological flow based on the component structure of ecological protection objects and dynamic hierarchical adjustment to the annual flow process
Authors: Wang Wenpeng , Tang Ming , Li Yanlei , Xu Wentao , Liu Longlingfeng , Liu Yanfu , Liu Jian , Yang Chundi , Wu Qiang , Ai Huili 
Published: March 2023

Abstract
Although regional and seasonal water scarcity occurs frequently in China, and the contradiction among domestic, production and ecological water is prominent in some watersheds, the Chinese government still attaches great importance to the determination and implementation of ecological flow of rivers or lakes. Practitioners have been seeking methods to determine the ecological flow of rivers or lakes and how to ensure its implementation. Taking the Dingnan River watershed as a case, drawing on the experience of ‘Hedging rule’, the ‘Determination-Assessment-Reduction’ for the ecological flow nexus approach (the D-A-R approach) is introduced, which includes the determination of the annual ecological flow process through the river section, the assessment of water scarcity degree of the watershed and various water reduction strategies, respectively, and respond to the three scenarios of ‘general type, saving type and constrained type’ during the gap period. The results show that it is possible to use the D-A-R approach to proactively and dynamically adjust the ecological flow according to the probability estimate of that amount of water inflow per month, which the adjusted ecological flow threshold can better adapt to water scarcity at different levels and alleviate the contradiction among domestic, production and ecological water in the watershed during the dry period.
Water supply strategies for higher land and water productivity in tank command areas of Southern India
Authors: Ramya B. , Rao B. Krishna , Kumar G. Manoj , Lakshmi Y. Siva 
Published: March 2023

Abstract
Tanks are one of the most important traditional sources of water for irrigation and other livelihood purposes in southern India. The present study was conducted to develop the strategies for enhancing the land and water productivity in tank command areas in the rain-fed regions of Telangana. Three strategies were identified, these strategies include change in the cropping pattern, water saving technologies and conjunctive use of tank with open well/bore well water. Due to change in cropping pattern from paddy to irrigated, dry crops like maize, chillies and cotton increased the cultural command area of the tanks by 79% and net reruns by 43%. The conjunctive use of tank water and well water is the better strategy for optimal resource use and higher returns in tank command areas. Results also showed that the alternate wetting and drying strategy in tank command areas increased the command area by 35%. These strategies can be implemented to increase the water use efficiency, cultural command area and net returns in the tank command areas of Southern India.
Impact of deficit irrigation on citrus production under a sub-humid climate: a case study
Authors: Panigrahi Pravukalyan 
Published: March 2023

Abstract
Water scarcity has resulted in lower yield (7–8 t ha−1) and a decline in citrus orchards in central India. In this scenario, optimal deficit irrigation (DI) is a potential option to sustain citrus production. To optimize the DI schedule in relation to yield, water productivity, fruit quality and production economics, water was applied at 30% of full irrigation (FI, 100% crop evapotranspiration), 50% of FI and 70% of FI and compared with FI in drip-irrigated citrus at Nagpur, India during 2008–2010. Fruit yield under 50% FI (11.48 t ha−1) was statistically at par with FI (13.14 t ha−1). However, a 50% reduction in water supply (1,343 m3 ha−1) resulted in a 75% improvement in water productivity in DI at 50% FI than FI (water productivity, 4.89 kg m−3). The fruit quality in DI at 50% FI (acidity, 0.83%; total soluble solids, 10.3 °Brix) was superior to FI (acidity, 0.85%; total soluble solids, 9.80 °Brix). The highest net profit (INR 94,300 ha−1) and net economic water productivity (70.19 INR m−3) were generated under DI at 50% FI. The study inferred that irrigation at 50% FI could be a water-saving and profitable option for citrus production in central India.
Ultrafiltration Rate Levels in Hemodialysis Patients Associated with Weight-Specific Mortality Risks
Authors: Mermelstein Ariella , Raimann Jochen G. , Wang Yuedong , Kotanko Peter , Daugirdas John T. 
Published: March 2023

Background
We hypothesized that the association of ultrafiltration rate with mortality in hemodialysis patients was differentially affected by weight and sex and sought to derive a sex- and weight-indexed ultrafiltration rate measure that captures the differential effects of these parameters on the association of ultrafiltration rate with mortality.

Methods
Data were analyzed from the US Fresenius Kidney Care (FKC) database for 1 year after patient entry into a FKC dialysis unit (baseline) and over 2 years of follow-up for patients receiving thrice-weekly in-center hemodialysis. To investigate the joint effect of baseline-year ultrafiltration rate and postdialysis weight on survival, we fit Cox proportional hazards models using bivariate tensor product spline functions and constructed contour plots of weight-specific mortality hazard ratios over the entire range of ultrafiltration rate values and postdialysis weights (W).

Results
In the studied 396,358 patients, the average ultrafiltration rate in ml/h was related to postdialysis weight (W) in kg: 3W+330. Ultrafiltration rates associated with 20% or 40% higher weight-specific mortality risk were 3W+500 and 3W+630 ml/h, respectively, and were 70 ml/h higher in men than in women. Nineteen percent or 7.5% of patients exceeded ultrafiltration rates associated with a 20% or 40% higher mortality risk, respectively. Low ultrafiltration rates were associated with subsequent weight loss. Ultrafiltration rates associated with a given mortality risk were lower in high–body weight older patients and higher in patients on dialysis for more than 3 years.

Conclusions
Ultrafiltration rates associated with various levels of higher mortality risk depend on body weight, but not in a 1:1 ratio, and are different in men versus women, in high–body weight older patients, and in high-vintage patients.
Proteinuria and Exposure to Eculizumab in Atypical Hemolytic Uremic Syndrome
Authors: ter Avest Mendy , Steenbreker Hilbert , Bouwmeester Romy N. , Duineveld Caroline , Wijnsma Kioa L. , van den Heuvel Lambertus P.W.J. , Langemeijer Saskia M.C. , Wetzels Jack F.M. , van de Kar Nicole C.A.J. , ter Heine Rob ,   on behalf of the CUREiHUS Study Group
Published: March 2023

Background
Eculizumab is a monoclonal antibody for the treatment of atypical hemolytic uremic syndrome (aHUS). Kidney damage, a common condition in patients with aHUS, may result in proteinuria. Because proteinuria may affect the pharmacokinetics of therapeutic proteins such as eculizumab, the aim of our study was to investigate the effect of proteinuria on eculizumab pharmacokinetics.

Methods
This study was an ancillary study of a previously performed pharmacokinetic-pharmacodynamic study of eculizumab in aHUS. Proteinuria, measured as urinary protein-creatinine ratios (UPCR), was investigated as covariate for eculizumab clearance. Thereafter, we evaluated the effect of proteinuria on the exposure to eculizumab in a simulation study for the initial phase and for a 2-weekly and 3-weekly interval in the maintenance phase.

Results
The addition of UPCR as a linear covariate on clearance to our base model resulted in a statistically improved fit (P < 0.001) and reduction of unexplained variability in clearance. From our data, we predicted that in the initial phase, 16% of the adult patients with severe proteinuria (UPCR >3.1 g/g) will have inadequate complement inhibition (classical pathway activity >10%) on day 7 of treatment, compared with 3% of the adult patients without proteinuria. None of the pediatric patients will have inadequate complement inhibition at day 7 of treatment. For the 2- and 3-weekly dosing intervals, we predicted that, respectively, 18% and 49% of the adult patients and, respectively, 19% and 57% of the pediatric patients with persistent severe proteinuria will have inadequate complement inhibition, compared with, respectively, 2% and 13% of the adult patients and, respectively, 4% and 22% of the pediatric patients without proteinuria.

Conclusions
Severe proteinuria is associated with a higher risk of underexposure to eculizumab.

Clinical Trial registry name and registration number:
CUREiHUS, Dutch Trial Register, NTR5988/NL5833
Insight into clear aligner therapy protocols and preferences among members of the American Association of Orthodontists in the United States and Canada
Authors: Abu-Arqub Sarah , Ahmida Ahmad , Da Cunha Godoy Lucas , Kuo Chia-Ling , Upadhyay Madhur , Yadav Sumit 
Published: March 2023

ABSTRACTObjectivesTo investigate aligner treatment protocols among orthodontists in the United States and Canada and assess the factors influencing clinician choices in aligner systems, treatment protocols, and targeted malocclusions for aligners.Materials and MethodsA validated online questionnaire was developed specifically for this research and consisted of three sections. Section 1 evaluated demographics and experience with aligners. Section 2 assessed patient selection and demands and clinician confidence in treating various malocclusions with aligners. Section 3 evaluated treatment protocols used by clinicians. The American Association of Orthodontists Partners in Research Program distributed the survey via e-mail to active members in the United States and Canada.ResultsA total of 160 providers completed the survey. Aligners were used by 65.00% of respondents, with the Invisalign system the most popular (81.25%). Aligners were mostly used for adults (97.50%). Tipping was ranked as the easiest movement (1.79 ± 1.35). Extrusion (4.34 ± 1.53) and root movement (4.31 ± 1.27) were ranked as the most difficult. Most were confident treating mild (98.8%) and moderate (82.5%) crowded cases, spacing (96.9%), and anterior crossbite (85%). Of the providers, 58.12% recommended aligners to be changed weekly. Respondents who were confident addressing some of the severe malocclusions were more likely to use Invisalign.ConclusionsInvisalign is the most popular aligner system, and clinicians seem to be confident using it. Providers are aware of the pitfalls of aligners; they find it challenging to perform root movement and extrusion, and they seem confident treating mild to moderate malocclusions. They avoid complex cases with impactions and severe skeletal problems.
Glucose-activated switch regulating insulin analogue secretion enables long-term precise glucose control in type 1 diabetes mice
Authors: Xie Lifang , Lu Wanling , Zhang Yanhan , Deng Lu , Liu Ming , Gao Hong , Xie Chunguang , Wang Gang 
Published: March 2023

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<p>Genetic modification of non-β cells to produce insulin is a promising therapeutic strategy for type 1 diabetes, however, it is associated with issues including biosafety and precise regulation of insulin supply. In this study, a glucose-activated single-strand insulin analogue (SIA) switch (GAIS) was constructed to achieve repeatable pulse activation of SIA secretion in response to hyperglycemia. In the GAIS system, the CAD-FCS-SIA fusion protein was encoded by the intramuscularly delivered plasmid and temporarily kept in the endoplasmic reticulum (ER) as it binds to the GRP78 protein, then upon hyperglycemia, SIA was released and secreted into the blood. In vitro and in vivo experiments systematically demonstrated the effects of the GAIS system, including glucose-activated and repeatable SIA secretion, long-term precise blood glucose control, recovered HbA1c level, improved glucose tolerance, and ameliorated oxidative stress. Additionally, this system offers sufficient biosafety as evidenced by the assays of immunological and inflammatory safety, ER stress, histological evaluation, etc. Compared with the viral delivery/expression system, the <em>ex vivo</em> implantation of engineered cells, and the exogenous inducer system, the GAIS system combined the advantages of biosafety, effectiveness, persistence, precision, and convenience, providing therapeutic potential for the treatment of type 1 diabetes.</p>
Evaluating Non-IgE-Mediated Allergens’ Immunoreactivity in Patients Formerly Classified as “Intrinsic” Asthmatics with Help of the Leukocyte Adherence Inhibition Test
Authors: Olivier Celso Eduardo , G. Pinto Daiana , M. Teixeira Ana P. , S. Santana Jhéssica L. , P. G. Santos Raquel A. , Lima Regiane P. S. , S. Monteiro Everton 
Published: March 2023

Background: The ancient concept of “intrinsic” and “extrinsic” asthma has evolved in line with growing scientific knowledge and the use of new clinical diagnostic tools.
Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in asthmatic patients without evidence of IgE-mediated hypersensitivity against these allergens.
Methods: A group of 127 “intrinsic” asthmatic outpatients, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of fungal extracts.
Results: Cascade graphs were assembled according to the distribution of the LAIT results among the range of results of Leukocyte Adherence Inhibition (LAI) inside each group. A column graph was plotted with the mean LAI results for each antigen. The non-parametric Wilcoxon-Mann-Whitney U-test performed between the results of each test was significant only between the results of the LAIT performed with the dog extract and the mixed fungal extract (U = 1,179.5; the z-score was -2.64109; the p-value was 0.0083). All other Wilcoxon-Mann-Whitney U-test calculations between LAIT performed with the other allergens were not significant at p < 0.01.
Conclusion: The evidence of non-IgE-mediated immunoreactivity in allergic patients has been reported in the medical literature since the beginning of the 19th century and has been increasing since then. This descriptive article presents the contribution of the LAIT as a tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients formerly classified inside the “intrinsic” asthma endotype.