bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant growth promoting endophytic fungi of the wild halophytic rice Oryza coarctata
Authors: Airin Arifa Akhter , Arafat Md Iyasir , Begum Rifat Ara , Islam Md Rakibul , Seraj Zeba Islam 
Published: June 2023

Abstract
Background
Plant growth-promoting endophytic fungi (PGPEF) associated with halophytes have the potential to boost plant growth and salinity tolerance in crops enabling cultivation in the coastal lands.
Methods
Endophytic fungi from the wild halophytic rice Oryza coarctata were isolated, characterized, identified and studied for their effects on plant growth and yield both with and with without salt stress.
Key results:
In this study, three different fungal endophytes were isolated from the halophytic wild rice Oryza coarctata. Two isolates were identified as Talaromyces adpressus (OPCRE2) and Talaromyces argentinensis (OPCRh1) by ITS region sequencing, the remaining isolate NPCRE2 was identified as a novel strain named Aspergillus welwitschiae Ocstreb1 (AwOcstreb1) by whole genome sequencing. These endophytes showed various plant growth-promoting (PGP) abilities in vitro (e.g. IAA, ACC-deaminase and siderophore production, phosphate and zinc solubilization as well as nitrogen fixation), where AwOcstreb1 was significantly more efficient compared to the other two isolates at high salinity (900 mm). Independent treatment of these fungi in commercial rice (Oryza sativa) showed significant elevation in plant growth, especially in the case of the AwOcstreb1 inoculants, which had enhanced metabolite and chlorophyll content at the seedling stage in both no-salt control and 100 mm salt-stressed plants. At the same time, a significantly low level of H2O2, electrolyte leakage and Na+/K+ ratio was demonstrated under the saline condition. Higher expression (1.6 folds) of the SOS1 (Salt Overly Sensitive 1) gene was also observed in AwOcstreb1-treated plants under salinity stress. This strain also improved percent fertility, tillering, panicle number and filled grain number in both no-salt control and 45 mm salt-stressed inoculated plants at the reproductive stage. Consequently, their yield increased by 125.16% and 203.96% (p < 0.05) in colonized plants in normal and saline conditions, respectively, compared to uninoculated controls.
Conclusions
We propose that AwOcstreb1 is a potential candidate for an eco-friendly biofertilizer formula to improve cultivation and yield of rice or any crop in high saline coastal regions of Bangladesh.
Isolation of Pseudomonas aeruginosa from Food and Determination of Their Antibiotic Resistance
Authors: YILMAZ Nazife , URGANCI Nida Nur , YILDIRIM Zeliha 
Published: June 2023

Abstract
Despite the fact that Pseudomonas aeruginosa is as a prominent opportunist pathogen, it is a recondite microorganism in food safety. Due to its high adaptation capability, rapid reproduction and minimal growth necessities, P. aeruginosa is a common bacterium in the food chain. The resistance of P. aeruginosa to various antibiotics in clinical isolates has been extensively investigated and in-depth characterized. Conversely, information on food-borne isolates of this genus and species is insufficient. Therefore, in this study, 208 P. aeruginosa strains were isolated from 144 food samples collected from different provinces, and 10 isolates (from milk samples) with P. aeruginosa-specific characteristics were identified according to their 16S rRNA region. Antibiotic resistances of the identified isolates were determined against azithromycin (macrolides), erythromycin (macrolides), cephoperazon (cephalosporin), ceftazidime (cephalosporin), meropenem (carbapenem), imipenem (carbapenem), amikacin (aminoglycoside), teminoglycosylcin (teminoglycoside), teminoglycosylcin (pinocycycin) antibiotics. In this study, P. aeruginosa isolates were isolated from milk and the results showed that the prevalence of antibiotic resistance was low. It was found that all of the isolates showed 100% sensitivity to imipenem, piperacilin and vancomycin, 90% sensitivity to cephaperazon and meropenem, and 80% sensitivity to amikacin, ceftazidime and gentamycin. The same isolates were found to be 50% resistant to azithromycin, 90% resistant to tetracycline, and 100% resistant to erythromycin, nalidixic acid, ampicillin and chloramphenicol.
Long-term association of 15-second heart rate variability with cardiovascular events
Authors: Orini Michele , Duijvenboden Stefan van , Young William J. , Ramírez Julia , Jones Aled R. , Hughes Alun , Tinker Andrew , Munroe Patricia B. , Lambiase Pier D. 
Published: June 2023

Abstract
Heart rate variability (HRV) is an established cardiac autonomic marker with predictive value in cardiac patients. Ultra-short HRV (usHRV) derived from 10–30 sec ECGs can be measured at scale using standard and wearable ECGs, but its association with cardiovascular events in the general population is undetermined. We aimed to validate usHRV measured using 15-second ECGs (using RMSSD, SDSD and PHF indices) and investigate its association with atrial fibrillation (AF), major adverse cardiac events (MACE), stroke and mortality in individuals without cardiovascular disease. In the National Survey for Health and Development (n = 1,337 participants), agreement between 15-second and 6-minute HRV, assessed with correlation analysis and Bland-Altman plots, was very good for RMSSD and SDSD and good for PHF. In the UK Biobank (n = 51,628 participants, 64% male, median age 58), after a median follow-up of 11.5 (11.4–11.7) years, incidence of outcomes ranged between 1.7% and 4.3%. Non-linear Cox regression analysis showed that reduced usHRV was associated with all outcomes. Individuals with low usHRV (< 20th percentile) had hazard ratios for outcomes between 1.16 and 1.29, p < 0.05, with respect to the reference group. In conclusion, usHRV from 15-second ECGs correlates with standard short-term HRV and predicts increased risk of cardiovascular events in the general population.
Research on 3D Point Cloud Segmentation based on KCA- PointNet Network
Authors: Wang Wensheng , Jing Haitao , Liu Panpan , Tang Jinying , Luan Wenfei 
Published: June 2023

Abstract
Currently, mainstream 3D point cloud segmentation methods still have shortcomings, which have an impact on the accuracy of point cloud segmentation. In particular, by using the MLP layer with shared weights, PointNet, and its improved version, generate redundant channel features when mapping 3D point cloud data to high-dimensional space. This study intended to improve the PointNet network and introduce the key point attention and channel attention mechanisms into the PointNet model to build the KCA-PointNet network. Taking the point cloud data generated via oblique photography as the experimental data, the experimental results show that 1) after adding the KCA module, the segmentation accuracy of buildings, parks, and other places is greatly improved; 2) the segmentation accuracy of the combined use of the KA and the CA module is about 3% higher than that when they are used independently; and 3) the different connection modes of the KA and the CA modules also have an impact on the accuracy, and upon connecting the two modules in series, the data first pass through KA and the segmentation effect after CA is better than that of the other two connection methods. Through the improvement of the PointNet network, this study not only eliminated the redundant channel features, but also extracted the key points in the point cloud, reduced the possibility of losing important information in the process of point cloud data sampling, and effectively improved the accuracy and efficiency of point cloud segmentation. Furthermore, the results of this study will help to broaden the application scope of deep learning in the field of point cloud data, promote the development and innovation of point cloud segmentation algorithms, and lay a scientific foundation for deep learning point cloud segmentation.
The differential physiological mechanism and ecological response of Pisolithus sp. mycorrhizal Pinus thunbergii to Chromium
Authors: Shi Liang , Liu Binhao , Zhang Xinzhe , Zhao Xuan , Shen Zhenguo , Chen Yahua 
Published: June 2023

Abstract
Aims Pisolithus sp. as a class of ectomycorrhizal (ECM) fungi with multiple HM tolerance. However, the physiological mechanism and ecological response of Pisolithus sp. ECM plants to chromium (Cr) remain unclear. Pisolithus sp.1 (Cr tolerant strain) and Pisolithus sp.2 (Cr sensitive strain) ECM Pinus thunbergii were synthesized to explore their different physiological and ecological response to Cr in this work.
Methods Biomass, nutrient elements and Cr concentration of plants; Cr form and content in soil; Physicochemical properties, enzyme activities and microbial high-throughput analysis of soil were performed by pot and acute exposure experiments.
Results The growth and Cr tolerance of P. thunbergii were improved after been inoculated with Pisolithus sp.1 by reducing the transportation of Cr from roots to shoots, increasing the N assimilation rates of host through mycelium, and enhancing the available phosphorus (P) and enzyme activities in soil. The above-mentioned process were co-driven by the fungi in Basidiomycetes and Ascomycota phylum, the predominant bacteria Brevundimonas sp., Muribaculaceasand Lachnospiraceae, as well as hexavalent chromium (Cr(VI)) tolerant and reducing strain Simplicillium and Pichia. Pisolithussp.2 promoted the Mg accumulation in shoots of host and increased the abundance of Gibberella, Mortierella and Didymella in soil, which accelerated the P solubilization in soil and nutrient transformation in host to response to Cr.
Conclusions Our results showed that Pisolithussp.1 ECM P. thunbergii had high adaptability to Cr, and this work also have an important theoretical guiding significance and application value for mycorrhizal phytoremediation of Cr-contaminated soil.
Assessing the relationship between gut microbiota and oropharynx cancer:a two-sample Mendelian randomization analysis
Authors: GAO CHEN , LIN XIUAN , Wu YU , LIU HUI 
Published: June 2023

Abstract
Background Growing evidence suggests that gut microbiota is closely related to the risk of oropharynx cancer (OPC), but whether there is a causal effect remains unknown. In this study, we adopted a Mendelian randomization (MR) approach to evaluate the potential causal relationships between gut microbiota and the risk of OPC.Methods We identified genetic instrumental variables for gut microbiota from a genome-wide association study (GWAS) of 18,340 participants. Summary statistics of oropharynx cancer were drawn from a GWAS Catalog including 9,981 cases and 437,924 controls. Our main analyses were conducted using the inverse-variance weighted (IVW) method with random-effects model, complemented by the other two analyses: weighted median method and MR-Egger approach. We also performed reverse MR analysis to evaluate the possibility of reverse causation.Results We identified suggestive associations between four bacterial traits and the risk of OPC: the odds ratio (OR) was 2.74 (95% confidence interval [CI]: 1.57, 4.78; p = 0.00041) for genus Hungatella; OR was 0.53 (95% CI: 0.33, 0.85; p = 0.0087) for family Rikenellaceae; OR was 0.44 (95% CI: 0.23, 0.84; p = 0.012) for genus Alistipes, and OR was 0.56 (95% CI: 0.38, 0.82; p = 0.0029) for phylum Lentisphaerae. The results of sensitivity analyses for these bacterial traits were consistent. We did not find statistically significant associations between OPC and these four bacterial traits in the reverse MR analysis.Conclusions Our systematic analyses provide evidence to support a potential causal relationship between several gut microbiota taxa and the risk of oropharynx cancer. Further studies are required to investigate how the gut microbiota affects the development of OPC.
C-source priority and availability limits bidirectional electron transfer in freshwater mixed culture EAB biofilms
Authors: Michalska Karina , Brown Robert Keith , Schröder Uwe 
Published: June 2023

Abstract
This study investigated if a mixed electroactive bacterial (EAB) culture cultivated heterotrophically at a positive applied potential could be adapted from oxidative to reductive or bidirectional extracellular electron transfer (EET). To this end, a periodic potential reversal regime between − 0.5 and 0.2 V vs. Ag/AgCl was applied. This yielded biofilm detachment and mediated electroautotrophic EET in combination with carbonate, i.e. dissolved CO2, as the sole carbon source, whereby the emerged mixed culture (S1) contained previously unknown EAB. Using acetate (S2) as well as a mixture of acetate and carbonate (S3) in addition to small amounts of ethanol as carbon sources yielded alternating electrogenic organoheterotropic and electroautotrophic EET. The EAB mixed cultures developed accordingly, with S1 being very different in composition to S2 and S3, whereby S2 and S3 biofilms remained attached to the electrodes. There was only minor evidence of fully reversible bidirectional EET. In conclusion the three triplicates operated under the same conditions, but fed with different carbon sources demonstrated two forms of diauxie: Firstly, S1-BES showed a preference for the electrode as the electron donor via mediated EET. Secondly, S2-BES and S3-BES showed a preference for acetate as electron donor and c-source, as long as this was available, switching to CO2 reduction, when acetate was depleted. Ethanol is neutral and didn’t benefit either electrogenic organoheterophy or electroautotrophy.
Simulation and prediction of the geographical distribution of five Caragana species in the north temperate zone
Authors: Jiang Xiuchen , Wu Linxuan , Yang Guang , Gao Yike , Li He 
Published: June 2023

Abstract
Shrub encroachment caused by Caragana does harm to grassland ecosystem. However, based on the current limited understanding of ecosystems and future climatic conditions, the future distribution and dynamics of grassland-shrubland still need further research. In this paper, multivariate statistical analysis was used to identify the dominant environmental causes affecting the geographic range of five species of Caragana in the north temperate zone. Through the MaxEnt model, the current and future geographical distributions of them were simulated by combining 11 environmental variables and 1124 geographical distribution data points selected to analyze their geographical distribution patterns and dynamic changes. The results show the following: (1) The distribution patterns of the five Caragana species in the north temperate zone are predominantly governed by the abiotic factors of temperature and precipitation. (2) Caragana’s suitable habitat has a tendency to extend northward in the future. (3) As greenhouse gas emissions intensify, the habitable zone of C. microphylla and C. korshinskiiwill expand gradually, while the distribution probability of C. stenophylla, C. spinifera and C. pygmaea will shrink significantly in 60%-80% of the area, and the habitable area will fluctuate sharply. (4) The future distribution of suitable habitats for Caragana is concentrated in central Mongolia and around Lake Baikal in Russia. This research could offer a reference for the protection of grassland resources, ensuring sustainable development under the action of shrub encroachment.
Edaravone promotes A1/A2 polarization of astrocytes through JAK2 / STAT3 pathway
Authors: Liu Teng-teng , DUAN zhaoda , Jia Qiu-ye , Chen Hao-lun , Jia Wen-ji , Zheng Li-yang , Xu Dong-yao , Yang Yu-jia , Yuan Yun , Yang Li , Wu Chunyun 
Published: June 2023

Abstract
Edaravone is widely used in acute stroke. Regulation of astrocyte activation by edaravone is thought to be one of the neuroprotective mechanisms of neuroprotection of edaravone after ischemic injury. Previous studies have shown that astrocytes are rapidly activated after cerebral ischemia and differentiation into a neuroprotective A1 phenotype and a damaging A2 phenotype. Therefore, the present study aim to find the effects of edaravone on the A1/A2 phenotype of activated astrocytes after cerebral ischemia and the underlying regulatory mechanisms. For this purpose, we replicated the cerebral ischemia model and the oxygen-glucose deprivation induced astrocytes model. Western blot and immunofluorescence were used to detect the expression changes of A1 astrocyte markers C3 and SerpinG1, and A2 astrocyte markers PTX3 and SPHK1, respectively. We found that edaravone could significantly decreased the expression of C3 and SerpinG1 and enhanced PTX3 and SPHK1 expression in A2 astrocytes. Importantly, the JAK2/STAT3 pathway was activated after astrocyte activation, and edaravone treatment significantly inhibited the expression of proteins associated with this pathway. In light of the above, edaravone can promote the transformation of A1 astrocytes to A2 phenotype by regulating JAK2/STAT3 pathway and play a neuroprotective role.
Disturbance Rejection Model Predictive Control of Lower Limb Rehabilitation Exoskeleton
Authors: Jin Xin , Guo Jia 
Published: June 2023

Abstract
Nowadays, exoskeleton is broadly used in the rehabilitation training of many postoperative patients. However, the uncertainty and disturbances caused by different patients and system itself may lead to incompletely rehabilitation training as planned, or even unsafety. This paper addresses the control problem of a lower limb exoskeleton, in the spirit of the recent progress on Model Predictive Control (MPC) and Extended State Observer (ESO). More precisely, our approach is based on the strategy that designing an ESO to estimate the total disturbance of the dynamics model and compensating it in the design of the MPC process. To accomplish this, we introduce the virtual control quantity to decouple the dynamics model of the system and summarize the human disturbances, unmeasured states and system non-linearity as the total disturbance of the model. By doing so, the uncertainty can be estimated by our designed ESO. Based on the moving horizontal optimization and feedback mechanism of MPC, the output prediction of the system can be more accurate since the uncertainty are effectively compensated. The virtual experiment results demonstrate that proposed controller significantly improves the control accuracy on lower limb rehabilitation exoskeleton with disturbances (improved by over 34%), comparing with conventional MPC and fuzzy PID. As a result, our achievements will make contributions to better rehabilitation training for patients using rehabilitation exoskeletons.
Quantitative analysis of DNA methylation-related proteins in patients with knee osteoarthritis
Authors: Cai Zhengyu , Long Teng , Wang You 
Published: June 2023

Abstract
Background
Knee osteoarthritis is a common musculoskeletal disease characterized by degeneration of articular cartilage. Current diagnostic tools are limited to radiographs and physical examination.
Methods
We collected osteoarthritis cartilage tissues of five patients at different OA stage. We performed genome-wide DNA methylation sequencing and proteomic assays. For proteomic data processing, raw data from LC-MS/MS were imported into Proteome Discover 2.4 (Thermofisher) for analysis. For DNA methylation data processing, statistical differences in continuous variables between the two groups were compared by independent-samples t test. The possible causative genes were identified by cross-analysis of the two tests
Results
We identified 13 potential genes as osteoarthritis-related genetic signatures after analysis of the methylation and proteome.
Conclusions
Our study found that the process of knee osteoarthritis may be regulated by a combination of epigenetic and genetic modulation.
Self-efficacy and well-being in the association between caregiver burden and sleep quality among caregivers of elderly patients having multiple chronic conditions in rural China: a serial multiple mediation analysis
Authors: Yang Ziyue , Sun Fengye , Zhao Lingrui , Hu Tingwei , Lin Xin , Guo Yu Fang 
Published: June 2023

Abstract
Background
Caregivers of elderly patients with multiple chronic conditions have heavy caregiver burden and poor sleep quality, which has an important impact on both caregivers and patients. This study aimed to examine among rural caregivers of elderly patients who have multiple chronic conditions in China, whether self-efficacy and well-being mediate the link between caregiver burden and sleep quality.
Methods
The study recruited 325 caregivers of elderly patients having multiple chronic conditions in rural China. This current study utilized several measures including the Caregiver Burden Inventory (CBI), Athens Insomnia Scale (AIS), General Self-Efficacy Scale (GSES) and Index of Well-Being (IWB) to collect data from participants. Structural equation modeling was employed to study the relationships in caregiver burden, sleep quality, self-efficacy, as well as well-being.
Results
Results indicate self-efficacy and well-being act as mediators in the link between caregiver burden and sleep quality, accounting for 10.94% and 15.63% of the total effect, respectively. In addition, results suggest that self-efficacy and well-being have a serial multiple mediating effect, with this mediating pathway, explaining 9.93% of the total effect.
Conclusions
Caregivers of elderly patients having multiple chronic conditions in rural China experience poor sleep quality due to the caregiver burden. Self-efficacy and well-being have serial mediating roles between caregiver burden and sleep quality.
Research on compound combination of Realgar-Indigo naturalis Formula to reverse APL arsenic resistance by regulating autophagy through mTOR pathway
Authors: Li Ruibai , Xue Chengyuan , Pan Yiming , Li Guangda , Huang Ziming , Xu Jing , Zhang Jingfang , Chen Xinyi , Hou Li 
Published: June 2023

Abstract
Background: Acute promyelocytic leukemia (APL) used to be the most fatal type of acute myeloid leukemia (AML). After the emerge of all-trans retinoic acid and arsenic trioxide treatment, the Complete Response (CR) rate could reach 90%. In China, compound Realgar-Indigo naturalis Formula (RIF) are used instead of arsenic trioxide for home therapy. It has the advantages of reduced hospitalization time and more favorable price, and the CR rate is not lower than that of all-trans retinoic acid and arsenic trioxide therapy. However, drug resistances greatly affect the prognosis of patients. Compared with all-trans retinoic acid, previous studies on arsenic resistance are more limited.
Methods: In this study, the stable expression of arsenic-resistant cell line HL60-PMLA216V-RARα was established, and the mechanism of RIF reversing APL arsenic resistance was studied.
Results and Conclusion: We illustrated that the synergistic effect of RIF can regulate autophagy through the mTOR pathway, enhance cell apoptosis, promote cell differentiation, reduce mitochondrial membrane potential, and degrade arsenic-resistant PMLA216V-RARα.