bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines
Authors: Thomas Connon I. , Ryan Melissa A. , Kamasawa Naomi , Scholl Benjamin 
Published: July 2023

AbstractPostsynaptic mitochondria are critical to the development, plasticity, and maintenance of synaptic inputs. However, their relationship to synaptic structure and functional activity is unknown. We examined a correlative dataset from ferret visual cortex within vivotwo-photon calcium imaging of dendritic spines during visual stimulation and electron microscopy (EM) reconstructions of spine ultrastructure, investigating mitochondrial abundance near functionally- and structurally-characterized spines. Surprisingly, we found no correlation to structural measures of synaptic strength. Instead, we found that mitochondria are positioned near spines with orientation preferences that are dissimilar to the somatic preference. Additionally, we found that mitochondria are positioned near groups of spines with heterogeneous orientation preferences. For a subset of spines with mitochondrion in the head or neck, synapses were larger and exhibited greater selectivity to visual stimuli than those without a mitochondrion. Our data suggest mitochondria are not necessarily positioned to support the energy needs of strong spines, but rather support the structurally and functionally diverse inputs innervating the basal dendrites of cortical neurons.
Comparative genomics of clinicalStenotrophomonas maltophiliaisolates reveals regions of diversity which correlate with colonization and persistence in vivo
Authors: McDaniel Melissa S. , Sumpter Nicholas A. , Lindgren Natalie R. , Billiot Caitlin E. , Swords W. Edward 
Published: July 2023

ABSTRACTStenotrophomonas maltophiliais a Gram-negative emerging opportunistic pathogen often found in respiratory diseases such as cystic fibrosis (CF). Patients with CF experience lifelong polymicrobial infections of the respiratory mucosa. Our prior work showed thatP. aeruginosapromotes persistence ofS. maltophiliamouse respiratory infections. As is typical for environmental opportunistic pathogens,S. maltophiliahas a large genome and a high degree of genetic diversity. In this study, we evaluated the genomic content ofS. maltophilia,combining short and long read sequencing to construct complete genomes of 10 clinical isolates which were then compared with the larger phylogeny ofS. maltophiliagenomic sequence data, and compared colonization/persistence in vivo, alone and in coinfection withP. aeruginosa. We found that while the overall genome size and GC content were fairly consistent, there was considerable variability in arrangement and gene content. Similarly, there was significant variability inS. maltophiliacolonization and persistence in vivo in experimental mouse respiratory infection. Ultimately, this study gives us a greater understanding of the genomic diversity ofS. maltophiliaisolated from patients, and how this genomic diversity relates to interactions with other pulmonary pathogens, and to host disease progression. Identifying the molecular determinants of infection withS. maltophiliacan facilitate development of novel antimicrobial strategies for a highly drug-resistant pathogen.
Bacterial and fungal components of the gut microbiome have distinct, sex-specific roles in HawaiianDrosophilareproduction
Authors: Medeiros Matthew J. , Seo Laura , Macias Aziel , Price Donald K. , Yew Joanne Y. 
Published: July 2023

AbstractGut microbiomes provide numerous physiological benefits for host animals. The role of bacterial members of microbiomes in host physiology is well-documented. However, much less is known about the contributions and interactions of fungal members of the microbiome even though fungi are significant components of many microbiomes, including those of humans and insects. Here, we used antibacterial and antifungal drugs to manipulate the gut microbiome of a Hawaiian picture-wingDrosophilaspecies,D. grimshawi, and identified distinct, sex-specific roles for the bacteria and fungi in microbiome community stability and reproduction. Female oogenesis, fecundity and mating drive were significantly diminished when fungal communities were suppressed. By contrast, male fecundity was more strongly affected by bacterial but not fungal populations. For males and females, suppression of both bacteria and fungi severely reduced fecundity and altered fatty acid levels and composition, implicating the importance of interkingdom interactions on reproduction and lipid metabolism. Overall, our results reveal that bacteria and fungi have distinct, sexually-dimorphic effects on host physiology and interkingdom dynamics in the gut help to maintain microbiome community stability and enhance reproduction.
Flagellar motor remodeling during swarming requires FliL
Authors: Partridge Jonathan D. , Dufour Yann , Hwang YuneSahng , Harshey Rasika M. 
Published: July 2023

SUMMARYFliL is an essential component of the flagellar machinery in some bacteria, but a conditional one in others. The conditional role is for optimal swarming in some bacteria. During swarming, physical forces associated with movement on a surface are expected to exert a higher load on the flagellum, requiring more motor torque to move. Bacterial physiology and morphology are also altered during swarming to cope with the challenges of surface navigation. FliL was reported to enhance motor output in several bacteria and observed to assemble as a ring around ion-conducting stators that power the motor. In this study we identify a common new function for FliL in diverse bacteria –Escherichia coli, Bacillus subtilisandProteus mirabilis. During swarming, all these bacteria show increased cell speed and a skewed motor bias that suppresses cell tumbling. We demonstrate that these altered motor parameters, or ‘motor remodeling’, require FliL. Both swarming and motor remodeling can be restored in anE. coli fliLmutant by complementation withfliLgenes fromP. mirabilisandB. subtilis, showing conservation of swarming-associated FliL function across phyla. In addition, we demonstrate that the strong interaction we reported earlier between FliL and the flagellar MS-ring protein FliF is confined to the RBM-3 domain of FliF that links the periplasmic rod to the cytoplasmic C-ring. This interaction may explain several phenotypes associated with the absence of FliL.
Proguanil and chlorhexidine augment the antibacterial activities of clarithromycin and rifampicin againstAcinetobacter baumannii
Authors: Wang Chuandong , Zhang Tingting , Wang Yan , Wang Yipeng , Pan Hongwei , Dong Xinyu , Liu Siyu , Cao Meng , Wang Shuhua , Wang Mingyu , Li Yuezhong , Zhang Jian , Hu Wei 
Published: July 2023

ABSTRACTThe emergence ofAcinetobacter baumanniiinfections as a significant healthcare concern in hospital settings, coupled with their association with poorer clinical outcomes, has prompted extensive investigation into novel therapeutic agents and innovative treatment strategies. Proguanil and chlorhexidine, both categorized as biguanide compounds, have displayed clinical efficacy as antimalarial and topical antibacterial agents, respectively. In this study, we conducted an investigation to assess the effectiveness of combining proguanil and chlorhexidine with clarithromycin or rifampicin against both laboratory strains and clinical isolates ofA. baumannii. The combination therapy demonstrated rapid bactericidal activity against planktonic multidrug-resistantA. baumannii, exhibiting efficacy in eradicating mature biofilms and impeding the development of antibiotic resistancein vitro. Additionally, when administered in conjunction with clarithromycin or rifampicin, proguanil enhanced the survival rate of mice afflicted with intraperitonealA. baumanniiinfections, and chlorhexidine expedited wound healing in mice with skin infections. These findings are likely attributable to the disruption ofA. baumanniicell membrane integrity by proguanil and chlorhexidine, resulting in heightened membrane permeability and enhanced intracellular accumulation of clarithromycin and rifampicin. Overall, this study underscores the potential of employing proguanil and chlorhexidine in combination with specific antibiotics to effectively combatA. baumanniiinfections and improve treatment outcomes in clinically challenging scenarios.IMPORTANCEA. baumanniihas emerged as a globally significant nosocomial pathogen due to its remarkable ability to acquire antibiotic resistance and develop biofilms on both biotic and abiotic surfaces. Recent research has demonstrated that the antidiabetic drug metformin has a potentiation effect on doxycycline and minocycline against certain multidrug-resistant bacterial pathogens, suggesting the potential of this biguanide agent as a novel tetracyclines adjuvant. In this study, we provide evidence showing that the combination of proguanil and chlorhexidine with clarithromycin or rifampicin exhibits rapid bactericidal activities against both planktonic cells and mature biofilms ofA. baumannii, the capacity to inhibit the development of antibiotic resistance and improvement of the treatment outcomes inA. baumannii-infected mice. Given the advantages of repurposing non-antibiotic drugs as antibiotic adjuvants, proguanil and chlorhexidine show promise as adjuvants of specific antibiotics in combating clinically significant pathogenicA. baumannii.
De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expression
Authors: Tangudu Naveen Kumar , Buj Raquel , Wang Hui , Wang Jiefei , Cole Aidan R. , Uboveja Apoorva , Fang Richard , Amalric Amandine , Sajjakulnukit Peter , Lyons Maureen A. , Cooper Kristine , Hempel Nadine , Snyder Nathaniel W. , Lyssiotis Costas A. , Chandran Uma R. , Aird Katherine M. 
Published: July 2023

Abstractp16 is a tumor suppressor encoded by theCDKN2Agene whose expression is lost in ∼50% of all human cancers. In its canonical role, p16 inhibits the G1-S phase cell cycle progression through suppression of cyclin dependent kinases. Interestingly, p16 also has roles in metabolic reprogramming, and we previously published that loss of p16 promotes nucleotide synthesis via the pentose phosphate pathway. Whether other nucleotide metabolic genes and pathways are affected by p16/CDKN2Aloss and if these can be specifically targeted in p16/CDKN2A-low tumors has not been previously explored. Using CRISPR KO libraries in multiple isogenic human and mouse melanoma cell lines, we determined that many nucleotide metabolism genes are negatively enriched in p16/CDKN2Aknockdown cells compared to controls. Indeed, many of the genes that are required for survival in the context of low p16/CDKN2Aexpression based on our CRISPR screens are upregulated in p16 knockdown melanoma cells and those with endogenously lowCDKN2Aexpression. We determined that cells with low p16/Cdkn2aexpression are sensitive to multiple inhibitors ofde novopurine synthesis, including anti-folates. Tumors with p16 knockdown were more sensitive to the anti-folate methotrexatein vivothan control tumors. Together, our data provide evidence to reevaluate the utility of these drugs in patients with p16/CDKN2A-low tumors as loss of p16/CDKN2Amay provide a therapeutic window for these agents.
Predicting on restaurant's hygiene rating: Does customer review emotion and content matter?
Authors: Jeon Jiyeon , Kim Eojina , Wang Xi , Tang Liang(Rebecca) 
Published: July 2023

PurposeThe hygiene factor is always imperative when customers consider a certain restaurant, and the information contained in customer reviews can be an efficient approach to gauge a restaurant's hygiene during gaps in the official inspection. Therefore, the purpose of this study was to investigate whether information obtained from online reviews could predict the upcoming hygiene rating, specifically, evaluating the impact of both qualitative and quantitative content of reviews on the restaurant hygiene rating.Design/methodology/approachThe quantitative research method with big data analytic techniques was applied in this study. In total, 127,656 pieces of data collected from 1,710 restaurants in four major cities in the USA were used in the analysis. Both quantitative factors (i.e. reviewer's numerical rating, days to review, readability, useful/funny/cool) and qualitative factors (i.e. eight emotional dimensions of textual reviews) were analyzed from the online customer reviews and considered in predicting the restaurant's hygiene rating.FindingsSix out of eight emotional dimensions including anger, disgust, fear, sadness, surprise and trust were identified as having significant influences on the restaurant hygiene ratings. While three quantitative variables including days to review, readability and usefulness were identified with significant impacts on the dependent variable of restaurant hygiene rating.Originality/valueThis study opens an avenue for innovative research that establishes a connection between customers' reviews and restaurants' inspection systems. The results allow restaurants to predict an impending hygiene inspection rating upon dynamic assessment of review content and aid in adjusting hygiene measures accordingly.
Inventory sharing in healthcare supply chains: systematic literature review and future research agenda
Authors: Atcha Panniphat , Vlachos Ilias , Kumar Satish 
Published: July 2023

PurposeIneffective management inventory of medical products such as blood and vaccines can create severe repercussions for hospitals, clinics or medical enterprises, such as surgery delays and postponements. Inventory sharing is a form of horizontal collaboration that can provide solutions to key actors of the healthcare supply chain (HSC), yet no prior study reviewed this topic.Design/methodology/approachThis study conducts a systematic literature review of thirty-nine inventory-sharing studies in the context of HSCs published from 2012 until early 2022. The descriptive and thematic analyses include chronological distribution, geographical location, comparison between developed/developing regions, stakeholder and incident analysis.FindingsThematic analysis classified inventory sharing among five product supply chains (blood, medical supplies, medicines, vaccines and generic medical products). Benefits include shortage reduction, cost minimisation, and wastage mitigation. Barriers include (1) IT infrastructure, (2) social systems, (3) cost and (4) supply chain operations. Perishable inventory policies include Fresher-First (FF), Last-Expire-First-Out (LEFO), First-In-First-Out (FIFO) and First-Expire-First-Out (FEFO). The analysis also showed differences between developed and developing countries. The study identifies several future research opportunities that include (1) product utilisation rate, (2) cost reductions, (3) shortage mitigation and (4) waste reduction.Originality/valueNo prior study has systematically reviewed inventory sharing in HSCs to reveal benefits, barriers, patterns and gaps in the current literature. It makes five propositions and develops a research model to guide future research. The study concludes with theoretical and managerial implications.
Does gratuitous behaviour promote workplace nonviolence? Exploring the mediating role of constructive deviance
Authors: Garg Naval , Sharma Nidhi 
Published: July 2023

PurposeThis study evaluates gratitude's role in developing nonviolent work behaviour. It also examines the mediating effect of constructive deviance in the relationship between gratitude and nonviolent work behaviour.Design/methodology/approachThe study is based on responses from 424 working professionals engaged in different Indian industries: banks, insurance, IT, manufacturing, hotel and software. The respondents were approached both physically and electronically using convenience sampling. Also, the data were collected in three phases four months apart, utilizing the benefits of a repeated cross-sectional research design. Structural equation modelling examines the relationship between gratitude and nonviolent work behaviour. Model fit indices are also assessed for two models (without a mediator and with a mediator). Total, direct and indirect effects are calculated using AMOS 21 to study the mediating effect of constructive deviance.FindingsFindings reveal that all three dimensions of gratitude (lack of sense of deprivation, simple appreciation and appreciation for others) are positively associated with nonviolent work behaviour. The results also confirm the mediating effect of constructive deviance.Originality/valueThis is one of the pioneer studies exploring gratitude's role in ensuring nonviolent work behaviour.
How prone are emerging markets' sectoral indices to global uncertainties? Evidence from the quantile connectedness approach with portfolio implications
Authors: Khan Shabeer , Rehman Mohd Ziaur , Shahzad Mohammad Rahim , Khan Naimat U , Abdul Razak Lutfi 
Published: July 2023

PurposeThere has been a burgeoning interest in exploring the impact of uncertainty factors on share returns. However, studies on the influence of global financial uncertainties on emerging market sectoral indices are scarce. Thus, there is a need to have a thorough investigation of the connection between global financial uncertainties and emerging market sectoral indices. To fill this gap, using the theoretical framework of international portfolio diversification (IPD) and utilizing data from 2008 to 2021, this study examines the spillover connection between global uncertainty indices (GUIs) and leading sectoral indices of 28 emerging markets.Design/methodology/approachThe authors employ the quantile spillover-based connectedness approach and minimum connectedness portfolio approach to explore the dynamic connectedness among sectoral indices and global uncertainty indices (GUIs) as well as portfolio implication.FindingsThe study found high connectedness among all indices, especially at higher and lower quantiles. Among GUIs, the authors find that stock market volatility (VIX) and oil volatility index (OVX) are strongly interconnected with all leading emerging markets' sectoral indices. Among sectoral indices, the linkage between the financial (F-Index), information technology (IT-Index), and consumer discretionary (CD-Index) sectors shows moderate interconnectedness. In contrast, the communication services (CS-Index) sector has low interconnectedness with the system. In terms of spillover effects, the authors find EVZ, OVX, and the IT sectors to be net recipients for the entire period. The authors also explored portfolio diversification benefits by employing a minimum connectedness portfolio approach. The cumulative returns' findings show a slight decline in the portfolio's value after 2010; during 2012, the pattern remained stable; from 2014 to 2020, the portfolio performed negatively, that is, underperformance due to different events in that period, including COVID-19. The Consumer Discretionary sector is found to be significant because of having the largest weight, 51%, in the portfolio during the study period.Practical implicationsThe study suggests that investors should invest in the communication services sector as it is the least connected. However, the connectedness increases during COVID-19, which implies that it may be difficult for investors to benefit from IPD in a crisis period. Hence, to obtain the benefits from IPD, the evidence suggests that investors need to consider Consumer Discretionary sector while considering assets for investment.Originality/valueThe study's uniqueness is that the authors have investigated spillover between GUIs and 28 emerging markets sectoral indices by employing a quantile spillover-based connectedness approach and minimum connectedness portfolio approach with a special focus on portfolio implication.
Impact pathways: just transition in fashion operations and supply chain management
Authors: Karaosman Hakan , Marshall Donna 
Published: July 2023

PurposeThis impact pathways paper proposes that operations and supply chain management (OSCM) can help to ensure that the transition from a high-carbon to low-carbon fashion industry takes place in a just, inclusive and fair way. By immersion in fashion brands, suppliers and workers' realities across multiple supply chains, the authors identify challenges and issues related to just transitions, whilst proposing research pathways to inspire future OSCM research and collaboration using innovative and creative methods to answer complex questions related to just transition.Design/methodology/approachThe research the authors introduce used a multi-level field research approach to investigate multiple fashion supply chains in transition.FindingsThe authors uncovered that in the pursuit of lowering carbon emissions, fast-fashion giants work with industrial associations to create top-down governance tools, leading to severe problems in supply chain data and paradoxical demands. These demands are cascaded onto the workers in these supply chains. The goals and tools dictated by the fashion giants exclude workers, whilst the physiological and psychological effects on the workers are routinely ignored. These issues impede a just transition to a low-carbon fashion industry.Originality/valueThe authors introduce concepts largely missing from OSCM literature and ensure representation of the most marginalised group, supply chain workers, in a novel setting in a call for research in this emerging area.
Does the integration of lean production and Industry 4.0 in green supply chains generate a better operational performance?
Authors: Maldonado-Guzmán Gonzalo , Pinzón-Castro Sandra Yesenia , Garza-Reyes Jose Arturo 
Published: July 2023

PurposeThe tightening of environmental measures and policies in various countries around the world is forcing manufacturing companies, particularly those that make up the automotive industry, to improve their production processes, through the implementation of approaches such as lean production (LP) and Industry 4.0 (I4.0) technologies, to reduce industrial waste. However, the literature indicates that the implementation of LP and I4.0 does not always lead to an improvement in the level of operational performance (OP). Therefore, this study analyzes the effects of the implementation of LP practices and I4.0 on a green supply chain (GSC) and the operational performance of manufacturing companies in the Mexican automotive industry.Design/methodology/approachA theoretical research framework consisting of six hypotheses was developed and validated by applying partial least squares structural equation modeling (PLS-SEM) and using a sample of 460 companies from the Mexican automotive industry.FindingsThe results show that the level of OP of manufacturing companies increases substantially with the implementation of LP and I4.0 practices, as well as a GSC.Practical implicationsManagers of manufacturing companies will be able to use the results of this study to improve their production systems and to demonstrate the effects of these practices on OP.Originality/valueThis study contributes to the literature on LP and I4.0 by providing robust empirical evidence of the positive effects of implementing these approaches on the GSC and OP of manufacturing companies.