bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and Safety of varying dosage of Gefapixant in Patients with chronic cough. A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
Authors: Hudaib Muhammad , Tabassum Nabira , Malik Hurais , Tariq Umer Bin , Klair Nimra , Abedin Mariam Farhan Essa , Zafar Fatima , Abdullah Muhammad , Shahzad Mohammad Saiem , Syed Abdul Rehman Shah , Rabbani Samra , Khatri Mahima , Kumar Satish 
Published: January 2025

Abstract

Background
A cough that remains unresolved for longer than 8 weeks is referred to as chronic cough, and it is estimated that 10% of the adult population of the world suffers from chronic cough. Gefapixant is a purinergic receptor P2x3 receptor antagonist. P2x3 receptors are ATP-gated ion channels on the peripheral sensory C fibers in the airways.
Objective
We conducted a systematic review and meta-analysis of RCTs to investigate whether the chronic cough patients would benefit from gefapixant administration or not when compared to placebo.
Methods
We selected only those double-arm randomized controlled trials (RCTs) which included participants presenting with a chronic cough in patients 18 years or older. We excluded all the articles published in languages other than English Language. Furthermore, all the studies other than RCTs were also omitted. Articles yielded from the electronic search were exported to EndNote Reference Library software to remove any duplicates. Analyses were done using the Review Manager 5.4 tool. Mean values and standard deviations were retrieved for the continuous outcomes given as raw data.
Results
8 RCTs selected for the statistical analysis consisted of 2024 participants (1011 in the intervention group and 1013 in the placebo group). The outcomes resulted to be significant with 24-hour cough frequency, the mean difference [MD] = -5.26, 95% confidence interval [CI] -7.18 to -3.33; p = 0.00001; n = 3518, awake cough frequency (c/h) [MD] = -6.78, 95% confidence interval [CI] -9.46 to -4.09; p = 0.00001; n = 3472 patients., night cough frequency (c/h) [MD] = -4.07, 95% confidence interval [CI] -5.49 to -2.65; p = 0.00001; n = 479, patients cough severity VAS (mm) [MD] = -14.84, 95% confidence interval [-19.92, -9.76], p = 0.00001, n= 1297, cough severity diary difference [MD] = -0.85; 95% confidence interval [CI]: -1.14, -0.56; p-value = 0.00001; n = 1219, Total LCQ score MD=2.04, 95% CI = 1.09 to 2.98, p<0.0001, n =869.
Conclusion
To conclude, the lowest efficacious dose was 50 mg twice daily and helped reduce 24-hour cough frequency, awake cough frequency, night cough frequency, and cough severity using VAS in a dose-dependent manner. Gefapixant showed a significant risk of adverse events which include dysgeusia, hypogeusia, nausea, any adverse event, taste-related and drug-related adverse events, and serious AEs
Enhanced stability and efficacy of a lyophilized mRNA SARS-CoV-2 vaccine incorporating novel Ionizable lipids after one year storage at 25ºC
Authors: Mata Elena , Broset Esther , Matute Carlos , Stoian Andrei Mihai , Adame Susana , Alejo Teresa , López Alexandre , Andrés Beatriz , Heredero Juan , de Miguel Diego , Giménez-Warren Javier , Lampaya Verónica , Casabona Diego , Calvo Alba , Quincoces Gema , Peñuelas Iván , Gamazo Carlos , Uranga-Murillo Iratxe , Peña Natacha , Arias Maykel , Pardo Julian , Moreno Bernardino , Badiola Juan , Martínez Juan , Pérez Esther 
Published: January 2025

Abstract

mRNA vaccines have shown great efficacy against SARS-CoV-2, yet challenges remain in optimizing vaccine components to achieve enhanced immune response and vaccine stability. In this study, we developed CPVax-CoV, a new lyophilized mRNA vaccine that features novel thiolactone-based ionizable lipids and newly designed untranslated regions (UTRs) for enhanced expression. Incorporation of these optimized components into our vaccine candidate CPVax-CoV significantly improved immune responses in mice compared to commercially available mRNA vaccines. Moreover, lyophilized CPVax-CoV has proven to be thermostable, maintaining its biological activity for up to one year at 4°C and 25°C after lyophilization, overcoming the cold-chain limitations of current mRNA vaccines. This vaccine demonstrates protective efficacy against ancestral SARS-CoV-2 and Omicron XBB variant, offering a scalable solution for global distribution and pandemic preparedness. These findings underscore the potential of this platform for future next-generation mRNA vaccine development.
A survival prognostic model for gastro-intestinal lymphomas
Authors: Wu Shaojie , du junru , Ma Jie , Huang Li , Peng Zhiqiang , Ling Yiwen , Deng Xiaofang , Zhu Weijian , Li Hongliang , Wu Ming , Li Di , Guo Jing , Liao Ming , Gale Robert , Wang Hua , Li Yuhua 
Published: January 2025

Abstract

Primary gastro-intestinal lymphomas are the most common extra-nodal lymphomas but there is no widely-accepted survival prognosis model. We interrogated data from 1 023 consecutive newly-diagnosed people with primary gastro-intestinal lymphoma. The most common sites were stomach (55%). Diffuse large B-cell lymphoma (DLBCL) was the most common diagnosis (65%) followed by mucosa associated lymphoma (MALT) (20%). In people with DLBCL, survival of those with stomach or large intestine lymphomas was better compared with those with small intestine lymphomas (P = 0.03) and multiple sites (P < 0.01). People with DLBCL receiving R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine and prednisone) had better survival compared with those receiving CHOP only (P < 0.001). In multi-variable analyses of the training cohort with DLBCL age (P < 0.001), Lugano stage (P = 0.03) and albumin (P = 0.04) and lactic dehydrogenase (LDH) concentrations at diagnosis (P = 0.01) were significantly-associated with 10-year survival. Results were tested in a validation cohort. Using these co-variates we constructed a survival prognosis nomogram model with C-statistics of 0.82 (0.77, 0.86) and 0.80 (0.74, 0.85) in the training and validation cohorts. Our model, if further validated, can be used to predict survival of people with primary gastro-intestinal lymphomas.
Improving Real-time 3D Reconstruction and Semantic Segmentation for AR Applications
Authors: Nong Yu 
Published: January 2025

Abstract

This paper presents an innovative approach to enhancing real-time 3D reconstruction and semantic segmentation for Augmented Reality (AR) applications using advanced Artificial Intelligence (AI) techniques. We introduce a novel hybrid architecture that combines a lightweight NeRF (Neural Radiance Fields) variant with a real-time semantic segmentation network. Our method achieves state-of-the-art performance in both 3D reconstruction accuracy and semantic understanding while maintaining real-time performance on mobile AR devices. Experimental results demonstrate significant improvements in reconstruction quality, segmentation accuracy, and computational efficiency compared to existing methods.
GPT vs Human Legal Texts Annotations: A Comparative Study with Privacy Policies
Authors: Cevallos-Salas David , Estrada-Jiménez José , Guamán Danny S. , Rodríguez David , Álamo José M. del 
Published: January 2025

Abstract

High-quality corpora of annotated privacy policies are scarce, yet essential for training, testing, and evaluating accurate machine learning models. However, elaborating new corpora remains an error-prone and resource-intensive task, heavily reliant on highly specialized and hard-to-find human annotators. Recent advancements in Generative Pre-trained Transformers (GPTs) open the possibility of using them to annotate privacy policies with performance comparable to that of human annotators, thereby streamlining the process while reducing human resource demands. This paper presents a novel method for annotating privacy policies based on a codebook, a well-designed prompt, and the analysis of logarithmic probabilities (logprobs) of a GPT's output tokens during the annotation process. We validated our method using the GPT-4o model and the well-known, open, multi-class, and multi-label OPP-115 corpus, achieving performance comparable to 80% of human annotators in segment-level annotation and matching 90% of human annotators in a full-text level annotation. Furthermore, incorporating logprobs analysis allowed the method to match the performance of all human annotators in full-text level annotation, suggesting that context enhances the task. These findings demonstrate the potential of our method to automate annotations with performance similar to human annotators while significantly reducing resource demands.
ZP3 Expression in Pancreatic Adenocarcinoma: Its Implications for the
Prognosis and Therapy
Authors: Lyu Guizhen , Li Dongbing 
Published: January 2025

Background:
The role of Zona pellucida glycoprotein 3 (ZP3) is unclear in pancreatic
adenocarcinoma (PAAD).

Objective:
This study aimed to explore the role of ZP3 in PAAD.

Methods:
A comparative analysis of ZP3 gene expression was performed to discern differences between
various types of cancer and PAAD, leveraging data sourced from The Cancer Genome Atlas
(TCGA). This study aimed to assess the role of ZP3 as a potential diagnostic marker for
PAAD. The relationship between ZP3 levels and clinical characteristics, as well as patient outcomes,
was scrutinized. Additionally, genomic enrichment analysis was carried out to uncover the
underlying regulatory mechanisms associated with ZP3. The study further delved into the association
of ZP3 with immune system interactions, checkpoint gene expression, Tumor Mutational Burden
(TMB), microsatellite instability (MSI), and tumor stemness index (mRNAsi). The aberrant
expression patterns of ZP3 in PAAD cell cultures were confirmed through the application of quantitative
reverse transcription PCR (qRT-PCR) techniques.

Results:
ZP3 exhibited aberrant expression in both pan-cancer and PAAD. A significant correlation
was observed between increased levels of ZP3 expression in PAAD patients and histologic
grade (p = 0.026). Elevated ZP3 expression in PAAD was found to be significantly associated
with poorer overall survival (p = 0.003), progression-free survival (p = 0.012), and disease-specific
survival (p = 0.002). In PAAD, the level of ZP3 gene expression was statistically significant (p
< 0.001) and recognized as a key determinant of patient prognosis. ZP3 exhibited associations
with various biological pathways, including primary immunodeficiency, oxidative phosphorylation,
and other pathways. ZP3 expression demonstrated correlations with immune infiltration, immune
checkpoint genes, TMB, MSI, and mRNAsi in PAAD. Moreover, a pronounced negative
correlation was detected between ZP3 expression levels and the therapeutic effectiveness of various
medications, including selumetinib, bleomycin, FH535, docetaxel, and tanespimycin, within
the context of PAAD. Elevated levels of ZP3 were consistently observed in cell line models of
PAAD.

Conclusion:
ZP3 has the potential to serve as a prognostic biomarker and therapeutic target for patients
with PAAD.
Implications of EBV-Encoded and EBV-Related miRNAs in Tumors
Authors: Awasthi Prankur , Kohli Arjun Singh , Dwivedi Manish , Hasan Saba 
Published: January 2025

Abstract:
Over 90% of people are infected with the human g-herpesvirus known as the Epstein-
Barr virus (EBV). Cancers, such as gastric carcinoma, non-Hodgkin’s lymphoma, nasopharyngeal
carcinoma, Hodgkin’s lymphoma, and Burkitt lymphoma, are thought to be linked with EBV. It is
noteworthy that the first virus discovered that encodes microRNAs (miRNAs) was EBV, and these
miRNAs show expression at the different phases of EBV infection. There is growing evidence that
EBV-encoded miRNAs influence the growth of EBV-associated tumors. These EBV miRNAs, i.e.,
BamHI-H rightward fragment 1-derived microRNAs (BHRF1miRNA) and BamHI-A rightward
fragment-derived microRNAs (BART miRNAs), are crucial for the persistence of viral infection
and the avoidance of host defenses. Currently, significant advancements have been made in
analyzing the microRNAs that are found in the duration of EBV infection, in vitro studies identified
molecular targets of miRNAs and in vivo studies enhanced our understanding regarding the
pathophysiology of these molecules. An extensive look into the pro-carcinogenic impact of
microRNAs associated with EBV will increase our understanding of the molecular mechanisms of
EBV-associated tumors. In this paper, we have highlighted the functions of miRNAs in EBV
infection as well as recent developments in miRNA-based therapeutic and diagnostic approaches
that could be useful for EBV-related malignancies. Significantly, targeted therapies against EBV
miRNAs are advancing rapidly, with emerging approaches such as miRNA sponges, anti-miRNA
oligonucleotides, and CRISPR/Cas9 technologies. These innovations indicate the imminent onset of
a new era in the treatment of EBV-associated tumors.
Ginsenoside Compound C Targets Prediction for Intervertebral Disc Degeneration Treatment
Authors: Zhang Yizhi , Wei Ziyan , Wang Dongxin , Chen Shijie , Yang Fengguang , Jia Jingwen , Qiu Xiaoming , Kang Xuewen 
Published: January 2025

Background:
Intervertebral Disc Degeneration (IDD) is a major cause of lower back pain.
Ginsenoside compound C (GCC) has shown potential in treating IDD, but its specific targets and
mechanisms remain unclear.

Objective:
This study aimed to investigate the potential molecular mechanisms of GCC in the treatment
of IDD using network pharmacology and molecular docking approaches.

Method:
Public databases were utilized to identify 100 targets related to GCC and 20,757 targets
associated with IDD, of which 96 common targets were determined through cross-analysis. A Protein-
Protein Interaction (PPI) network was constructed using the STRING database, and key gene
clusters were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes
(KEGG) analyses were performed to explore the biological processes and pathways involved. Molecular
docking was conducted to verify the binding of GCC to key proteins.

Results:
Six key genes, including HSP90AA1, CASP3, MAPK1, EGFR, HIF1A, and PIK3CA, were
identified as central to the PPI network. GO analysis suggested that GCC's targets are involved in
intracellular processes, while KEGG analysis highlighted the IL-17 and TNF signaling pathways.
Molecular docking confirmed stable binding between GCC and key proteins, with binding energies
ranging from -3.58 to -2.01 Kcal/mol.

Conclusion:
GCC may inhibit the IL-17 and TNF signaling pathways by interacting with key proteins,
potentially contributing to the treatment of IDD.
Beyond the Surface: Tracing the Evolution of Inflammatory Mechanism in Depression through Bibliometric Analysis
Authors: Xu Zhang-Yang , Zhang Ting , Gong Hong , Zheng Hong , Liu Mei-Shan , Guo Bing-Hang , Wang Yun-Xia , Su Wen-Jun 
Published: January 2025

Background:
Depression is a common mental illness that has become a major economic
burden worldwide. Recently, increasing evidence has highlighted the inflammatory mechanism
of depression. In order to understand the research status of this field, this study used the bibliometric
analysis method to overview the research content and progress, as well as analyze the development
trend and limitations.

Methods:
In this study, articles and reviews were included in the specific search strategy. The
matched papers were exported from the Web of Science database. CiteSpace 6.3 R1 and Bibliometrix
(R package) were utilized to generate bibliometric and knowledge maps.

Results:
A total of 25,063 articles were included in this study. The number of publications in this
field has gradually increased, especially in recent years. These papers come from 156 countries,
led by the United States and China mainland. The leading research institution is the University of
Toronto (Canada). Brain Behavior and Immunity is the journal with the most publications and the
most frequently co-cited journals. Among 91,100 authors, Maes M has the most publications and
co-citations. According to the keywords burst and co-cited reference analysis, the hotspots in the
field in recent years include kynurenine, c-reactive protein, neuroinflammation, and gut microbiota.

Conclusion:
Although abundant academic achievements have been made on the inflammatory
mechanism of depression, there is still a long way to go before these research results can be applied
to clinical practice. Strengthening international academic exchanges and cooperation may
promote breakthroughs in this field.
Protective Effects of Hydrogen Treatment Against High Glucose-Induced Oxidative Stress and Apoptosis via Inhibition of the AGEs/RAGE/NF-κB Signaling Pathway in Skin Cells
Authors: Yu Pan , Hong Nan , Wu Qiong , Zhao Zhipeng 
Published: January 2025

Background:
Diabetic wounds are major clinical challenges, often complicated by
oxidative stress and free radical generation. Hydrogen (H2), a selective antioxidant, offers potential
as a therapeutic agent for chronic diabetic wounds. However, its precise mechanisms remain
underexplored.

Objective:
This study aimed to investigate the protective effects of H2 on high glucose-induced
oxidative damage and apoptosis in human skin cells.

Methods:
HaCaT keratinocytes and HSF fibroblasts were treated with high glucose or AGEs. Cell
viability, oxidative stress markers, inflammatory cytokines, and apoptosis were analyzed.
AGEs/RAGE/NF-κB signaling was evaluated via Western blot.

Results:
H2 treatment significantly reduced ROS, MDA, IL-1β, and TNF-α levels, while enhancing
SOD and GSH activity. It also inhibited AGEs/RAGE/NF-κB signaling and apoptosis.

Conclusion:
Hydrogen therapy protects against oxidative stress and inflammation induced by
high glucose or AGEs, offering potential as an adjunctive treatment for diabetic wound healing.
Evaluation of Salivary and Serum micro RNA 146a, 200c and its Target Gene PTEN in Chronic Periodontitis Patients and their Response to Non-Surgical Periodontal Therapy
Authors: Prasanna Jammula Surya , Apoorva Kunnel 
Published: January 2025

Background:
Periodontitis destroys the tooth's supporting structures and attachment apparatus. Local or systemic factors can cause it. Traditionally, diagnosis is based on clinical parameters that may not consistently reflect an accurate confirmation. Biochemical and genetic analyses can provide deeper insights. MicroRNAs (miRNAs) regulate the immune and inflam-matory response to microbial pathogens. Detecting and evaluating miRNAs can be an important diagnostic parameter. This study aimed to assess the expression of miRNA 146a,200c, and its target gene PTEN to non-surgical periodontal therapy in serum and saliva.

Materials and Methods:
This interventional comparative study was conducted on 120 patients of both genders, ages between 35 and 55. Non-surgical periodontal therapy (NSPT) scaling and root planing were performed on all subjects, and their saliva and serum samples were collected before and after 8 weeks of NSPT. Quantitative rt-PCR (reverse transcriptase Polymerase Chain Reaction) analysis was conducted on all samples. The statistical analysis was done using SPSS version 22, and comparisons were made using paired t-tests, independent t-tests, and Pearson’s correlation coefficient. The statistical significance level was set at a ‘P’ value of less than 0.05.

Results:
It has been observed that there was a significant difference of miRNA in both serum and saliva samples 146a,200c, and the PTEN gene expression, from the beginning to 8 weeks. Sig-nificant variation was not observed when comparing the levels between serum and saliva.

Conclusion:
miRNA 146A, 200c, and PTEN genes are interrelated with periodontitis. We can consider them as future biomarkers of periodontal diseases.
Vitamin B12 Malabsorption: The Pathophysiological Impacts on Neurological and Hair Physiology
Authors: Rathore Prince Nikhil , Gupta Sakshi , Madhariya Rashmi , Ram Alpana 
Published: January 2025

Abstract:
Vitamin B12 (cobalamin) is a water-soluble vitamin. It is a crucial vitamin for the health
of humans, contributing immensely to numerous biological processes. This study aims to highlight
the importance of vitamin B12 in nourishing hair growth and the nervous system and how its malabsorption
might trigger various problems in the body. Major absorption of vitamin B12 takes place
in the small intestine from several foods like milk, eggs, fish, liver, meat, and yoghurt. Several factors
can cause malabsorption of vitamin B12, including atrophy of the gastric mucosa, disease of the
terminal ileum in the body, and cigarette smoking. These factors can lead to the demyelination of
large nerve fibers in the spinal cord, a variation in the fraction of S-adenosylmethionine to S-adenosylhomocysteine,
and an alteration in TNF and epidermal growth factor (EGF) levels, which can
cause optic neuropathy and hair loss. Maintaining a proper diet and leading a healthy lifestyle are
crucial for maintaining vitamin B12 levels in the body, as the body can store this vitamin for up to
3–6 years. Mainly, the level of vitamin B12 in the healthy human body is 160 to 950 picogrammes
per millilitre In conclusion, the factors leading to malabsorption of vitamin B12 and their consequent
deficiencies were examined. The body's numerous crucial functional pathways are influenced by
vitamin B12.
Biochar Amendment Alleviates the Risk of High-Salinity Saltwater Intrusion for the Growth and Yield of Rice (Oryza sativa L.)
Authors: Faiyue Bualuang 
Published: January 2025

Introduction:
Saltwater intrusion poses a serious risk to global food security. As a soil amendment, biochar mitigates the negative effects of saltwater intrusion in rice, yet the beneficial effects on agricultural productivity with different exposure times and salt concentrations have not been fully examined.

Methods:
A pot experiment was conducted to investigate the effects of 30% (w/w) rice husk biochar on the growth, ion accumulation, and yield of the Phitsanulok 2 rice cultivar under salt stress due to saltwater intrusion. Rice plants were grown in saline soil amended with biochar and were salinized with 6, 8, and 10 dS/m saltwater for 1, 2, and 3 months. A treatment without biochar was taken as the control.

Results:
The results showed that biochar amendment significantly increased the survival, shoot height, and tiller numbers of rice treated with the 6-10 dS/m saltwater for 1 and 2 months, as well as the treatment with 6 dS/m salt water for 3 months, in comparison with the control. The grain yield was about 40 g/pot for rice with biochar treated for 1 month with 6-10 dS/m saltwater and for the rice treated for 2 months with 6 dS/m.
The results indicated that biochar application could alleviate the intrusion of high-salinity water for 1 month of salt exposure, and it could alleviate the intrusion of medium-salinity water for 2 months.

Conclusion:
Therefore, a 30% (w/w) rice husk biochar amendment is a reliable strategy for mitigating the risks of saltwater intrusion for the growth and productivity of rice.