bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infection with Toxoplasma gondii triggers coagulation at the blood-brain barrier and a reduction in cerebral blood flow
Authors: Hoover Evelyn M. , Schneider Christine A. , Crouzet Christian , Lima Tatiane S. , Velez Dario X. Figueroa , Tran Cuong J. , Agalliu Dritan , Gandhi Sunil P. , Choi Bernard , Lodoen Melissa B. 
Published: January 2025

Abstract
Background
Immunothrombosis is the process by which the coagulation cascade interacts with the innate immune system to control infection. However, the formation of clots within the brain vasculature can be detrimental to the host. Recent work has demonstrated that Toxoplasma gondii infects and lyses central nervous system (CNS) endothelial cells that form the blood-brain barrier (BBB). However, little is known about the effect of T. gondii infection on the BBB and the functional consequences of infection on cerebral blood flow (CBF) during the different stages of infection.

Main body
We demonstrate that brain endothelial cells upregulate the adhesion molecules ICAM-1 and VCAM-1 and become morphologically more tortuous during acute T. gondii infection of mice. Longitudinal two-photon imaging of cerebral blood vessels during infection in mice revealed vascular occlusion in the brain, prompting an analysis of the coagulation cascade. We detected platelet-fibrin clots within the cerebral vasculature during acute infection. Analysis of CBF using longitudinal laser-speckle imaging during T. gondii infection demonstrated that CBF decreased during acute infection, recovered during stable chronic infection, and decreased again during reactivation of the infection induced by IFN-γ depletion. Finally, we demonstrate that treatment of mice with a low-molecular-weight heparin, an anticoagulant, during infection partially rescued CBF in T. gondii-infected mice without affecting parasite burden.

Conclusions
Our data provide insight into the host-pathogen interactions of a CNS parasite within the brain vasculature and suggest that thrombosis and changes in cerebral hemodynamics may be an unappreciated aspect of infection with T. gondii.
Two independent families with de novo whole APC gene deletion and intellectual disability: a case report
Authors: Iwaizumi Moriya , Taniguchi Terumi , Kojima Risa , Osawa Harumo , Tatsuta Kyota , Sakata Mayu , Osawa Satoshi , Kurachi Kiyotaka , Sugimoto Ken 
Published: January 2025

Abstract
Background
Familial adenomatous polyposis (FAP) is an autosomal dominant colorectal tumour syndrome characterised by the formation of multiple adenomatous polyps throughout the colon. It is important to understand the extracolonic phenotype that characterizes FAP. Most previous case reports of patients with both FAP and intellectual disability (ID) have described deletions in all or part of chromosome 5q, including the APC locus. However, it remains unclear whether the ID phenotype in patients with FAP is due to APC disruption or another genetic defect in the deleted 5q region.

Case presentation
Patient of family 1 is a 32-year-old woman presented with > 500 colorectal adenomatous polyps, gastric fundic gland polyposis, several duodenal adenomas, and mild intellectual disability (ID). She had no known family history of the FAP phenotype or ID. By copy number trio analysis, a 15.4 Mb interstitial heterozygous de novo deletion including APC region was observed in 5q21.2. q22.3. The patient in family 2 was a 29-year-old man with approximately 50 colorectal adenomatous polyps, fundic gland polyposis in the stomach, non-ampullary adenomas in the duodenum, and mild ID. He had no family history of the FAP phenotype or ID. Using copy number trio analysis, a de novo 9.8 Mb heterozygous deletion was identified on 5q22.1. q23.1 which includes the APC region.

Conclusions
Based on previous reports and the present study, we narrowed down the 5p deletion region associated with ID in FAP. Further investigation is required to understand ID due to 5q stromal deletion.
Late Miocene Tapiridae from Vallès-Penedès Basin (NE Iberian Peninsula): taxonomic and paleoenvironmental implications
Authors: Pandolfi Luca , Arranz Sara G. , Almécija Sergio , Galindo Jordi , Luján Àngel H. , Pina Marta , Urciuoli Alessandro , Casanovas-Vilar Isaac , Alba David M. 
Published: January 2025

AbstractEuropean Miocene tapirs (Perissodactyla, Tapiridae) are mainly documented by isolated and fragmentary remains, and little is known about the morphological variability of the various recognized species, in particular concerning the deciduous dentition. Here, we describe new material from three Vallesian (Late Miocene) sites of the Vallès-Penedès Basin (NE Iberian Peninsula): Creu de Conill 20 (CCN20; earliest MN9, 11.2 Ma), Can Llobateres 1 (CLL1; earliest MN10, 9.8 Ma), and Can Gambús-Illeta 5 (CGS-I5; MN10, 9.7–9.1 Ma). The described material, including previously reported specimens from CLL1, consists of a juvenile cranium as well as deciduous and permanent teeth, which are compared with those of Late Miocene tapirid species from Europe (Tapirus priscus, Tapirus antiquus, Tapiriscus pannonicus, Tapiriscus balkanicus, and Tapirus arvernensis), supporting an attribution to T. priscus. The Vallès-Penedès specimens provide further insight into the morphological variability of T. priscus, particularly regarding its deciduous dentition, as well as its distinction from T. arvernensis. Tapirus priscus occurred in Western Europe during the early and late Vallesian (MN9–MN10) and became extinct in Central Europe during the early Turolian (MN11), leaving a gap in the fossil record of Tapirus until the arrival of T. arvernensis at the MN13–MN14 transition. From a paleoecological perspective, the presence of Tapirus agrees with paleoenvironmental reconstructions of CLL1 as a humid and densely forested wetland/riparian forest with more open areas nearby, while the CGS-I5 material attests to the persistence of such kind of environments in the Vallès-Penedès Basin sometime into the late Vallesian. In contrast, the occurrence of Tapirus is at odds with previous paleoenvironmental inferences for CCN20, which indicated a more open and arid environment, suggesting that the earliest Vallesian in this basin was characterized by more humid and forested conditions than previously assumed (in further agreement with the roughly coeval site of Castell de Barberà).
Coping, meaning in life, and quality of life during ongoing conflict: insights from Israeli populations
Authors: Sarid Orly , Hamama Liat , Hamama-Raz Yaira 
Published: January 2025

Abstract
Background
Conducted in May 2024, this study examines the well-being of Israeli evacuees and non-evacuees from conflict zones. We assess health-related quality of life (HRQoL), meaning in life (MIL), coping strategies, psychological symptoms, and self-mastery. Aims include exploring effects of trauma and socio-demographics on HRQoL and MIL, analyzing mediating roles of psychological symptoms and coping, and evaluating if evacuation status moderates these relationships during ongoing conflict.

Methods
In May 2024, seven months post–October 7th attacks, we conducted a cross-sectional study with 366 participants (221 evacuated, 145 non-evacuated) via a survey company. We assessed HRQoL (SF-12), MIL (MLQ), psychological symptoms (PHQ-4), self-related and other-related coping strategies (Brief COPE), and self-mastery (Self-Mastery Scale) through self-reported measures. Path and moderated mediation analyses evaluated relationships among socio-demographics, psychological symptoms, coping variables, HRQoL, and MIL.

Results
Except for self-mastery, northern and southern evacuees showed no significant differences and were combined into one group. Path analysis revealed significant associations between traumatic life events, HRQoL, and MIL. Traumatic events were negatively associated with the physical component summary (PCS) of HRQoL and positively with anxiety, depression, and coping (self and others- problem-solving). Depression negatively related to PCS, mental component summary (MCS), and MIL, while coping (self and others) was positively associated with MIL. Moderated mediation analysis showed evacuated participants had higher dysfunctional coping, whereas non-evacuated participants demonstrated a stronger positive relationship between anxiety and the search for meaning.

Conclusion
Despite regional differences, evacuees exhibited similar psychological responses, likely due to the shared experience of displacement. Traumatic events negatively impacted their HRQoL and MIL. Adaptive coping strategies—self-related and problem-focused coping through helping others—played significant roles in mitigating these effects. The theoretical frameworks of Conservation of Resources (COR) theory, Taylor’s “tend and befriend” model, and Frankl’s existential framework provided a basis for explaining these findings.
Is there a significant relationship between the empowering leadership behaviors of school principals and the psychological resilience of teachers? Understanding the moderating effects of gender and length of time spent with the school principal
Authors: Çevik Mehmet Sabir , Doğan Emine 
Published: January 2025

AbstractThe psychological resilience of teachers has gained significant attention due to its positive effects on schools and students. This cross-sectional quantitative study investigates the moderating roles of gender and the length of time spent with the school principal in the relationship between principals' empowering leadership behaviors and teachers' psychological resilience. Data were collected from 362 teachers working in public primary, secondary, and high schools in Siirt, Türkiye. Descriptive statistics, correlation analysis, and moderation analysis using the bootstrapping method were applied to analyze the data. The findings reveal that the empowering leadership behaviors of school principals positively correlate with teachers' psychological resilience, with variations based on gender and time spent with the principal. Specifically, the relationship is stronger for female teachers compared to male teachers and for those who have spent more time with the school principal. These results offer theoretical insights and practical recommendations for enhancing teacher resilience through leadership practices, highlighting the importance of understanding contextual and demographic factors.
Serotype distribution and antimicrobial susceptibility of Streptococcus suis isolates from porcine diagnostic samples in Hungary, 2020–2023
Authors: Albert Ervin , Kis István Emil , Kiss Krisztián , K-Jánosi Katalin , Révész Tamás , Biksi Imre 
Published: January 2025

Abstract
Background
Streptococcus suis (S. suis) is a major swine pathogen and a significant zoonotic agent, causing substantial economic losses in the swine sector and having considerable public health importance. The control and management of S. suis-related conditions has become increasingly challenging due to the multitude of involved serotypes with varying antimicrobial resistance patterns. Here, we report the serological distribution and antimicrobial susceptibility of S. suis isolates isolated form clinical samples of Hungarian large-scale swine farms.

Results
Between 2020 and 2023, altogether 296 S. suis isolates were obtained from diseased pigs of 64 Hungarian pig operations. Serotyping of the isolates was carried out by using molecular methods (cps-typing). The isolated strains belonged to 24 single cps-types. The most frequently detected cps-types during the four years of this passive survey were 9 (19.6%), 2 (19.3%), 1/2 (18.9%) and 7 (14.5%). The brain, spleen, endocardial valve thrombus and lung proved to be the most frequent site of S. suis strain isolation, and animals 29–75 days of age were affected in the highest proportion.
Antimicrobial susceptibility testing of the isolates was performed by determining the minimal inhibitory concentration for 15 antimicrobial agents of veterinary and human importance using a commercial microdilution assay. More than 90% of the tested isolates proved to be susceptible to the examined beta-lactams, cephalosporins and florfenicol, as well as to rifampicin, trimethoprim/sulfamethoxazole and vancomycin. Phenotypic resistance profiles (resistotypes) of clindamycin-tetracyclin (3.8%), clindamycin-erythromycin-tetracyclin (8.4%) and clindamycin-erythromycin-tetracyclin-trimethoprim / sulfamethoxazole (3.8%) were most frequently detected. Vancomycin resistance was observed in the case of 1 S. suis strain.

Conclusions
The dominance of S. suis cps-types 9, 2, 1/2 and 7 in Hungary over the four years of this study aligns with previous reports from several countries worldwide. The presence of highly susceptible S. suis isolates suggests a prudent antibiotic usage and treatment practice in the surveyed Hungarian swine operations. In contrary, the presence of several resistotypes could indicate the problem of antibiotic resistance in the future.
Patient experience with eosinophilic esophagitis symptoms and impacts on daily life based on in-trial qualitative interviews
Authors: Ho Calvin N. , Fjällbrant Harald , Dellon Evan S. , Santander Cecilio , Rothenberg Marc E. , Bailey Julie , McFadden Shehan , Ritchie Jason 
Published: January 2025

Abstract
Purpose
Eosinophilic esophagitis (EoE), a chronic immune-mediated progressive disease, causes dysphagia, food impaction, abdominal pain, vomiting, and heartburn. EoE requires long-term monitoring and can affect quality of life owing to its symptoms and associated emotional and social burden. This study aimed to understand patients’ experiences with EoE.

Methods
Semi-structured longitudinal interviews were conducted with patients from MESSINA, a phase 3 placebo-controlled trial evaluating benralizumab for EoE. Interviews were held at two different times to assess the impact of EoE on patients’ lives before and during trial participation. Data were analyzed qualitatively to develop detailed patient profiles.

Results
The MESSINA trial was terminated prematurely. Of the 34 patients recruited for the first interview, 15 (44%) completed the second interview and 11 patient profiles were developed. Patients were a demographically diverse group with varying experiences. The primary reported symptom was difficulty swallowing (n = 11), leading to serious consequences like choking and hospitalization (n = 2). Other symptoms included pain when swallowing (n = 7), reflux (n = 6), and stomachache (n = 6). In the second interview, most (n = 9) patients reported moderate improvements in symptoms, while others experienced symptom recurrence or worsening. EoE had a significant negative impact on social and emotional well-being, and professional lives. Trial participation improved emotional well-being for some; however, concerns about the need for ongoing treatment were noted.

Conclusion
This study highlighted emotional and social burdens of EoE. The encouraging feedback on study participation underscores the importance of patient insights in developing holistic management strategies for EoE.
Evolutive radiological findings in a case of coccidioidal infection in an immunocompetent male: a case report
Authors: La Montanara Paolo , Albergo Annamaria , Capozzi Loredana , Castellana Roberto , Cazzato Gerardo , De Carlo Carmela , Denicolò Sofia , Netti Antonella , Poliseno Mariacristina , Ranieri Enrica Maddalena , Romano Caterina , Rossiello Annalisa , Stolfa Stefania , Tripoli Alessandra , Vella Giuseppe 
Published: January 2025

Abstract
Background
Bone infections such as chronic fungal erosive osteomyelitis are uncommon forms of bone infection. The endemic dimorphic fungus Coccidioides impact generally immunocompromised patients. These infections frequently have no symptoms and the clinical signs remain undetected, allowing the infection to worsen over weeks or months. Mycotic arthritis is one of the rarest clinical symptoms; it is hard to distinguish from other types of arthritis, which slows down the diagnosis procedure.
Case presentation
In order to demonstrate the beginning and progression of radiological abnormalities in a case of aggressive fungal osteomyelitis, we provide the case of a 31-year-old male patient here. The man showed signs of extensive bone erosion and inflammatory involvement in his right knee and right hallux phalanx, although he had no prior history of immunodeficiency. The infection resulting from Coccidioides Immitis in his right knee and in his hallux was the reason for the injuries.

Conclusions
While an acute, benign, and self-eradicating lung infection is the predominant presentation for most cases of coccidioidomycosis, a small percentage of patients experience a devastating extrapulmonary condition, which can include arthritis. The pathogenic mechanism of bone involvement is unknown, and it often remains untreated. Here, we discuss radiographic evidence of particular bone inflammation during the early phase and later phases of the disease, since management of this chronic condition remains a challenge. We propose that imaging may mimic osseous neoplasia in persistent fungal diseases, such as coccidioidomycosis.
Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation
Authors: Bhinge Somnath Devidas , Jadhav Sayali , Lade Pranali , Bhutkar Mangesh Anil , Gurav Shailendra , Jadhav Namdeo , Patil Abhinandan , Upmanyu Neeraj 
Published: January 2025

Abstract
Background
The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing Clerodendrum serratum L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration approach with varying concentrations of phospholipids (soya phosphatidylcholine) at 25, 50, and 100 mg, respectively, to evaluate their efficacy against skin cancer cell lines. Several analytical approaches, such as UV/Vis spectroscopy, XRD spectra, FTIR, TEM, SEM, particle size distribution, and zeta potential analysis, were employed to assess the synthesized CS-NTs.

Results
Experimental results revealed that CS-NTs exhibited a circular shape, with average vesicle sizes approximately measured at 463.6 ± 100.5 nm, 265.5 ± 61.6 nm, and 409.6 ± 106.2 nm for CS-NTs 25, CS-NTs 50, and CS-NTs 100, respectively. Notably, biologically synthesized CS-NTs 25 offer potential anticancer benefits against B10F16 (skin cancer cell line) cells, exhibiting no signs of adverse effects. The heightened biological potential of CS-NTs can be ascribed to their unique characteristics at the nanoscale, characterized by an average vesicle size and lower polydispersity value, which facilitate augmented responsiveness and interactions with biological systems.

Conclusion
CS-NTs 25 formulated through the biogenic process using C. serratum hydroalcoholic plant root extract hold significant potential for anticancer activity.

Graphical abstract
The Skin of Trees
Authors: Chanchani Nachiket 
Published: January 2025

Abstract
Informed by interviews, studio visits, exhibition openings, and on-site observation of a changing landscape, this essay argues that recent artworks by Sopheap Pich and several other Cambodian and Vietnamese artists prompt us to think of the materiality of plants, their taxidermy, and the possibility of laboriously creating plant–human amalgams without completely anthropomorphizing nature or partaking in the anthropomorphic transforming into the arboreal. This article also maintains that these contemporary Southeast Asian artists are willfully relinquishing some control over their artworks and letting form-making and meaning-making activities that also occur outside the human realm to transpire. By doing so, they are investigating, representing, and addressing grave social and environmental problems that the region has been experiencing since the 1970s.
Building Symbolic Capital through International Cultural Exchange
Authors: Liu Prynne Yan , Liu Jules Zhao 
Published: January 2025

Abstract
Gao Jianfu (1879–1951) is one of the most famous artists of twentieth-century China. His art and career have both attracted much scholarly attention. Nevertheless, despite a proliferation of studies, existing scholarship has ignored an important period in his career, specifically the period from 1927 to 1933. This article addresses this research gap. Drawing on newspaper articles, archival materials, and newly discovered letters between Gao and his friends, this study delineates Gao's career trajectory during this period. The article argues that this phase was a turning point in Gao's career, a time when he successfully turned himself from a discredited artist into an internationally acclaimed master. This change was achieved through his South Asian tour, during which he won great support from Pan-Asianist artists, reaped considerable symbolic capital, and built up his prestige and recognition in the Asian art world. This research enriches scholars’ understanding of an obscure period in Gao's artistic career and provides a new perspective on his ultimate success.
Therapeutic potential of bisphosphonates - review of medical indications, mechanism of action, interactions and adverse effects
Authors: Mikosińska Agnieszka , Litwin Mateusz , Parys Jakub , Kałuziak Patrycja , Witek Aleksandra , Kaźmierczak Martyna , Jesionek Stanisław , Jajczak Marta , Mossakowski Maciej 
Published: January 2025

Introduction: Bisphosphonates are pharmacological agents that inhibit bone resorption by targeting osteoclasts, thereby increasing bone density and reducing fracture risk. Although they were first synthesized in the 19th century, their clinical significance has only been recognized in the last 50 years. This study reviews the current understanding of bisphosphonates, their therapeutic advantages in various disorders, and their mechanism of action, potential interactions, and adverse effects.
Aim of the study: The aim of this study is to provide an overview of the current understanding of bisphosphonates and familiarize readers with their mechanisms of action, interactions and adverse effects. It also aims to briefly introduce diseases that may be treated with bisphosphonates and how this class of medications can improve the course of each disorder. This study focuses on their therapeutic potential and associated risks, highlighting the need for further research in this area.
Material and methods: Literature available in the PubMed database was reviewed using the following keywords:
“bisphosphonates”; “bisphosphonates mechanism of action”; “bisphosphonates side effects”; “bisphosphonates interactions”; “bone tissue”; “skeletal system disorders”; “skeletal system”; “menopause”;” osteogenesis imperfecta”; “bone metastasis”
Conclusions: The introduction of bisphosphonates represents a significant advancement in the management of conditions associated with increased bone turnover, offering clinically substantiated benefits that outweigh the associated risks. This review provides healthcare professionals with evidence-based, scientifically validated information on bisphosphonates, while also identifying critical areas for further investigation to optimize their safety and efficacy, ultimately improving patient outcomes and quality of life.