bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desain Primer Dan Deteksi Gen CHS (chalcone synthase) Pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb.) Tipe Riau Gadang
Authors: Wardi Epi Supri , Syukur Sumaryati , Chaidir Zulkarnain , Jamsari Jamsari , Sartika Diza 
Published: December 2022

Gambir merupakan salah satu tanaman yang mengandung katekin.Kandungan katekin pada gambir merupakan komponen yang menjadi syarat utama dalam penentuan mutu gambir. CHS (Chalcone synthase) adalah salah satu gen yang terlibat di dalam proses biosintesis pembentukan katekin. Penelitian ini bertujuan untuk mendapatkan primer yang dapat digunakan dalam deteksi gen CHS (Chalcone synthase) pada tanaman gambir tipe riau gadang serta untuk melihat kemampuan primer yang telah didesain untuk mendeteksi gen CHS pada tanaman gambir. Pendesainan primer dilakukan dengan mengalignment 21 data sekuengen CHS dan dipilih daerah yang memiliki kesamaan basa antara sekuen gen yang dialignment untuk mendapatkan primer. Isolasi DNA gen CHS tanaman gambir menggunakan metode CTAB dan untuk isolasi RNA menggunakan Total RNA Mini Kit (Plant) dari Geneaid. Sintesis cDNA menggunakan kit Rever Tra Ace® qPCR RT Master Mix with gDNA Remover (Toyobo).Pada hasil desain primer didapatkan empat primer forward dan satu primer reverse. Hasil desain primer yang dapat digunakan untuk deteksi gen CHS dengan hasil cDNA tanaman gambir yaitu primer F3-R1(TNGTCTTCTGCACNACCTCCGGNG - CCANTC CAASCCYTCWCCDGTSGT). Proses deteksi gen CHS pada daun gambir menghasilkan produk dengan estimasi sebesar 724 bp
Reduksi Katalitik 4-Nitrofenol dengan Katalis Nanopartikel Perak Terkonjugasi L-Lisine
Authors: Alfanaar Rokiy , Afthoni Muhammad Hilmi 
Published: December 2022

Nanopartikel perak berpotensi menjadi katalis homogen dalam reduksi 4-nitrofenol menjadi 4-aminofenol. Berbagai penelitian menunjukan L-lisine berpotensi bertindak sebagai agen penstabil nanopartikel perak sehingga mempengaruhi sifat katalitik nanopartikel perak. Pada penelitian ini dilakukan reduksi 4-nitrofenol menggunakan katalis nanopartikel perak yang terkonjugasi dengan L-lisini dengan reduktor NaBH4. Dari hasil reduksi didapatkan nanopartikel perak dengan dosis 50 ?L mampu mengubah 99,99% 4-nitrofenol menjadi 4-aminofenol dengan tetapan laju reaksi 0,1130 menit-1.
Manajemen Pendidikan Aqidah Akhlak MTs Al Munawwarah di Lingkungan Eks Lokalisasi Pekerja Sex Komersial (PSK) Desa Slarang Kecamatan Kesugihan Kabupaten Cilacap
Authors: Sudadi Sudadi 
Published: December 2022

Examines the education management process applied at MTs Al Munawwarah Kesugihan Cilacap Regency, as an effort to improve the morals of students who come from the ex-localized Slarang Commercial Sex Worker (PSK) environment. The purpose of this study is to describe the management of MTs The Impact on Students Who Come from the Ex-Localization of Slarang Commercial Sex Workers (PSK) in the 2019/2020 Academic Year. This research was conducted with a qualitative approach. The subjects of this study were the principal, teachers, and students. Data collection techniques from this study using the method of observation, interviews, and documentation. The results of this study indicate that, 1) Learning planning by teachers is carried out by compiling annual programs, semester programs, developing learning syllabus, and developing lesson plans. 2) Organizing learning is carried out by the teacher, namely: structuring classrooms, structuring students, using learning resources, using learning media, and using facilities and infrastructure. 3) Implementation of learning is the teacher carrying out learning activities including preliminary activities, core activities, and closing activities. 4) Learning evaluation is the principal of madrasah providing guidance, assistance, supervision and assessment on problems related to the technical implementation and development of teaching education in the form of improving teaching education programs and activities to create teaching and learning situations. Meanwhile, the teacher evaluates learning through process assessment and results assessment. Process assessment is carried out on student participation both individually and in groups during the learning process. Assessment of results is carried out at the middle and end of the semester. 5) Reporting of learning outcomes that the content of reporting learning outcomes includes things such as student learning profiles at madrasah (academic, physical, social and emotional), student participation in activities at madrasah (active, moderate, insufficient or inactive), progress of results student learning during a certain learning period, appeal to parents.
INTERPRETIVE PARADOXES OF TEXTUAL OPENNESS: NARRATOLOGICAL, PSYCHOANALYTIC, AND FEMINIST DISSECTION OF THE SHORT STORY “THE SINNER” BY LES MARTOVYCH
Authors: Lepokhin Yevhenii 
Published: December 2022

The scholarly debate over the writer’s outlook and individual literary style of Les Martovych (1871—1916) is still relevant. The ironic mood of his works and its genesis, the psychological and cultural foundation of his writings, the vision of Martovych’s narration in the context of examining his psychological ground – this is what modern researchers, in particular, Roman Pikhmanets, Olena Hnidan, Oleh Ilnytskyi, and Hanna Marchuk, most often reflected on. Just as topical is the interpretation of the compositional and semantic level of the writer’s texts along with the study of the peculiarities of using linguistic and stylistic means for the realization of the author’s intention in the translations of his works into foreign languages.
This paper focuses on the man-woman relationship in the short story “The Sinner” (1904) in terms of post-structural psychoanalytic, narratological, and feminist perspectives. Obvious is the gap between the notion of a woman as a feminine character and as a sociohistorical subject in a patriarchal world. The interpretation of the psychological portraits of the characters strives for defining the elusive nature of a reality that is driven by the ideology of patriarchy on the one hand, and the emancipation of women on the other.
Using the method of ‘suture’, the paper demonstrates the process of creating female and male subjectivity at the level of narrative discourse. The findings may serve those who research the Ukrainian literary trends of the late 19th — early 20th centuries, peculiarities of the development of Ukrainian prose of the mentioned period in relation to the general European context, feminist-psychoanalytic discourse in literature, and the mechanisms of creating female and male images in prose works.
“ITALIAN JOURNEY” BY J. W. GOETHE: BETWEEN WINCKELMANN AND HEGEL
Authors: Shalahinov Borys 
Published: December 2022

The text of the “Italian journey” analyzes the main objects of Goethe’s attention from the point of view of aesthetic systematization: natural phenomena (sea, mountains, vegetation, volcanoes), geological and botanical features of the area, various results of human activity and human behavior, abandoned and dilapidated architectural monuments of antiquity, ‘unconventional’ personalities. Panoramic contemplation of the area is seen as the main way of the author’s observations and the development of a specific worldview, which includes an attempt not only to separately consider the contradictory diversity of phenomena but also to harmonize it esthetically. Artistic allusions serve as one of the ways of such harmonization.
The behavior of the human crowd and traces of human activity are presented by the author in the same stream with the natural phenomena of nature. As to the cultural assessment of modern Italians, the author describes them as happy, naive, simple-minded, and carefree but indifferent to the requirements of modern civilization and the cultural achievements of their ancestors. Aesthetically, the poet-observer relies on Winkelmann’s artistic postulate of “noble simplicity and calm greatness” and tries to reveal the contradictions of this system in terms of the aesthetics of the emerging new age. However, in general, Goethe’s aesthetics remains within the framework of the eighteenth century and includes universal concepts of contemporary art: integrity, harmony, revitalization, diversity, balance, and others. Therefore, the terminological apparatus and range of concepts tend not to Hegel (heroic, dramatic, lyrical, comic, etc.) but rather to the previous era.
La colección inédita de remedios bizantinos en el códice de Galeno Urb. gr. 67
Authors: Durán Mañas Mónica , Pérez Martín Inmaculada 
Published: December 2022

Se presenta en este artículo la primera edición de una breve colección de recetas recogidas en el manuscrito Città del Vaticano, Biblioteca Apostolica Vaticana, Urb. gr. 67, un códice dedicado enteramente a Galeno y compuesto de dos partes bien diferenciadas. En su parte más antigua (ff. 53-275), datable hacia 1300, aparece esta colección de remedios de tradición galénica, que el copista ha añadido (ff. 186v-187v) para completar el cuaderno numerado ??’ (17) al final de la copia de Galeno, De compositione medicamentorum secundum locos.
Epidemiology and risk factors of nasal carriage of Staphylococcus aureus CC398 in two distinct cohorts in France
Authors: Bouiller Kevin , Zeggay Abdeljalil , Gbaguidi-Haore Houssein , Hocquet Didier , Chirouze Catherine , Bertrand Xavier 
Published: December 2022

BackgroundWe aimed to determine the prevalence and factors associated with nasal carriage of Staphylococcus aureus CC398 in the community and among hospitalized patients.MethodsWe conducted a prospective cohort study in a French university hospital and a cross-sectional study in the surrounding region.ResultsFrom June 2019 to July 2020, 591 healthy blood donors (HBDs) and 647 hospitalized patients (HPs) were included. S. aureus CC398 was more prevalent in HBDs than in HPs (7.3% [5.3–9.7] vs. 3.8% [2.4–5.5], p = 0.006). Among S. aureus nasal carriers, the prevalence of CC398 isolates was 24.6% in HBDs and 18.3% in HPs (p = 0.19). No MRSA belonged to CC398. In multivariate analysis, prior antibiotic intake in the past year (OR 3.11 [1.37–7.06]) and active smoking (OR 3.01 [1.00–9.05]) were associated with S. aureus CC398 nasal carriage in the HBD cohort. A history of neurological disease was associated with nasal carriage (OR = 5.43 [1.21–24.2]), whereas an age between 82 and 90 years (OR 0.11 [0.02–0.54]) and diabetes (OR 0.18 [0.04–0.85]) were protective factors in the HP cohort. Contact with livestock was not a risk factor in either cohort.ConclusionThe prevalence of MSSA CC398 was higher in the community than hospitalized patients. Factors associated with nasal carriage of MSSA CC398 were primarily related to general preconditions. No environmental sources of exposure were identified.
Associations of serum carotenoids with the severity of sunburn and the risk of cancer: A cross-sectional analysis of 1999–2018 NHANES data
Authors: Cheng Bin , Wu Xixin , Li Ruina , Tu Jiayuan , Lin Sixian , Zhang Xiangda , Mo Xiaoqiao , Xie Tian 
Published: December 2022

BackgroundSunburn is a common problem for outdoor workers and casual outdoor walkers. Carotenoids are important elements in normal function of skin tissue and skin metabolism and are critical in the development of some cancers. However, the possible relationships between sunburn sensitivity, carotenoids and the risk of cancers remain unknown.ObjectivesTo explore the associations of serum carotenoids with sunburn severity and the risk of cancers.MethodsA cross-sectional study from the National Health and Nutrition Examination Survey from 1999 to 2018 were conducted. The relationship between sunburn and serum carotenoids, cancers were investigated by unconditional or ordinal logistic regression. Mediation analysis was used to explore the effect of carotenoids on the relationship between sunburn and cancers.ResultsA total of 25,440 US adults from 1999 to 2018 were enrolled in this study. There were significant differences in sex, race and natural hair color between the sunburn and non-sunburn people. The severity of sunburn was significantly associated with serum trans-β-carotene, cis-β-carotene, combined lutein, and vitamin A. The odds ratios of severe reactions were 5.065 (95% CI: 2.266–11.318) in melanoma patients, 5.776 (95% CI: 3.362–9.922) in non-melanoma patients, and 1.880 (95% CI: 1.484–2.380) in non-skin cancers patients. Additionally, serum carotenoids were partially attributable to the effect of sunburn on skin and non-skin cancers.ConclusionSunburn severity was associated with cancers, and severer sunburn was related with higher risk of cancers. Serum carotenoids were also associated with sunburn severity. Moreover, the relationship between sunburn and cancers was mediated by some serum carotenoids.
Ultrasonic misdiagnosis of giant pediatric testicular yolk sac tumor: A case report and literature review
Authors: Wang Zilong , Lu Fuding , Song Changze , Wang Xinkun , Li Naifa , Zhai Jiawen , Jiang Baohong , Yuan Jianpeng , Yang Zheng , Xuan Xujun 
Published: December 2022

BackgroundYolk sac tumor is the most common malignant nonseminomatous germ-cell tumor in children characterized by elevated level of α-fetoprotein (AFP), accounting for 70%–80% of all cases. However, giant yolk sac tumors that involve the entire testicle may be misdiagnosed by color Doppler ultrasonography as orchitis. Therefore, we described a case of a 2-year-old pediatric patient with a giant testicular yolk sac tumor that was misdiagnosed by ultrasonography as orchitis, in order to evaluate the role of measuring AFP levels in the initial diagnosis to aid in the accuracy of the definitive diagnosis of testicular yolk sac tumor.Case presentationA 2-year-old boy received outpatient visits for unintentional swelling of the right scrotum for 7 days. Physical examination showed a rubbery swelling of the right scrotum with rejective touch. Then, the patient underwent perineal color Doppler ultrasonography in outpatient visits. The result showed a right testicle size of 29 mm × 22 mm × 20 mm with heterogeneous echogenicity and abundant blood ﬂow, supporting the initial diagnosis of orchitis. However, the initial surgeon was skeptical of the ultrasonography diagnosis. Thus, the patient was admitted to the Department of Andrology on day 2 for further serological and imaging examination. The serum AFP level on day 3 was 323.77 ng/ml. The results of CT and MRI showed a giant tumor of the right testis (26 mm × 21 mm × 29.6 mm) with multiple lymphoid hyperplasia in the inguinal region bilaterally. The patient received radical orchidectomy without lymph node dissection on day 9. The results of postoperative pathological examination confirmed giant testicular yolk sac tumor (T1N0M0S1, Stage Is) and was positive for AFP and SALL4 in immunohistochemistry staining. The patient received three courses of bleomycin–etoposide–cisplatin chemotherapy in the Department of Pediatrics after multidisciplinary team meeting on postoperative days 14, 37, and 58, respectively. During chemotherapy and follow-up, the patient's AFP and lactate dehydrogenase levels continued to decline, and eventually remained within normal range on postoperative day 84.ConclusionMeasuring the AFP level was necessary for initial diagnosis and follow-up in pediatric cases of testicular enlargement. Radical orchidectomy combined with postoperative bleomycin–etoposide–cisplatin adjuvant chemotherapy was an effective treatment strategy for pediatric giant testicular yolk sac tumors.
Influence of the focusing characteristics of near-infrared lasers on the maintenance of plasma luminescence
Authors: Zhou Lian , Chen Yi , Guo Xin , Shi Yan , Zhao Tianqi , Zhan Chunlian , Jin Shangzhong 
Published: December 2022

The interaction of 980-nm continuous laser radiation with the plasma of a continuous optical discharge in xenon lamps at a pressure of p = 12 atm has been studied. The threshold power and characteristics of the laser required to sustain the xenon plasma became our focus. According to the theory of Gaussian beam propagation, the laser parameters after collimation and focusing are obtained by combining ZEMAX simulation and the actual measurement. The influence of the beam waist ω0, which determines the power density distribution at focus, and the Rayleigh range Z0, which determines the energy concentration range, on the threshold maintenance power is expounded. The results show that there is a threshold power density for the generation of plasma, whose value is about 1,500–2,000 W/mm2, and that the threshold maintenance power of the plasma shows an overall decreasing trend with decreasing ω0. When ω0 is reduced to a higher power density that can easily maintain the thermodynamic equilibrium process of the plasma, the mismatch between Z0 and the plasma size caused by the decrease in Z0 makes the threshold power tend to be stable and increasing.
Relationship between knee joint discomfort, self-management behavior, and quality of life in the middle-aged and elderly people in China: A cross-sectional study
Authors: Su Manman , Zhou Yang , Zhao Peipei , Zeng Biyun , Zhou Qidi 
Published: December 2022

BackgroundThe aim of this study was to describe the knee joint discomfort, self-management behavior, and quality of life (QoL) in the middle-aged and elderly people in China and to clarify the relationship between the knee joint discomfort, self-management behavior, and QoL.MethodsIt is a cross-sectional study and in this study, a stratified multistage random sampling method was used to collect data on the three factors such as knee joint discomfort, self-management behavior, and QoL among the middle-aged and elderly people in the Hunan Province. Spearman's correlation analysis was used to test the relationship between the knee joint discomfort, self-management behavior, and QoL.ResultsThe results of the present study showed that among them, the prevalence of knee pain was the highest (52.1%), followed by knee weakness (42.5%), numbness (41.8%), cold feeling (40.0%), tenderness (38.3%), and distension feeling (37.5%). Average score of self-management of knee joint discomfort in the middle-aged and elderly people was 2.14 ± 0.67. The level of self-management in each dimension ranged from high to low as emotional management, daily management, symptoms management, and information management. The average scores of physical component summary (PCS) and mental component summary (MCS) were 42.85 ± 5.34 and 43.62 ± 8.43, respectively. The occurrence, frequency, and severity of discomfort symptoms were positively correlated with the symptoms management, daily management, information management, and self-management behaviors, and negatively correlated with the emotional management, PCS and MSC, except for the occurrence of discomfort symptoms (P &lt; 0.05).ConclusionKnee joint discomfort was prevalent in the middle-aged and elderly people. In addition, they displayed a low level of self-management behavior and poor QoL. The middle-aged and elderly people faced knee discomfort symptoms, the more frequent and severe symptoms, the higher level of symptom management, daily management, and information management, and the lower level of emotional management and QoL.
Preserved visuoconstruction in patients with Alzheimer's pathology and anti-neural autoantibodies: A case control study
Authors: Hansen Niels , Hirschel Sina , Teegen Bianca , Wiltfang Jens , Malchow Berend 
Published: December 2022

BackgroundAlzheimer's disease (AD) is seldom reported to be associated with neural autoantibodies apart from those involved in axonal neurodegeneration and amyloidopathy in prior studies. Nevertheless, this is an under-investigated aspect of AD. As we do not know whether additional screening for autoantibodies in AD patients has additional diagnostic and therapeutic value, this study aims to shed light on whether visuoconstructive or figural memory capacities might distinguish these patient populations.MethodsIn this pilot case series, we investigated eight patients suffering from cognitive impairment associated with cerebrospinal fluid (CSF)-based Alzheimer pathology (AP) and with verified anti-neural autoantibodies (AP Aab+) compared to eight AD patients presenting no autoantibodies (Aab–) (AD Aab–). Patients files were reviewed retrospectively regarding their neuropsychological profile assessed via the CERAD (Consortium to Establish a Registry for Alzheimer's Disease) test battery and psychopathology measured by the AMDP (Manual for the Assessment and Documentation of Psychopathology in Psychiatry) system. We also relied on diagnostic parameters as in the CSF and magnetic resonance images.ResultsAll patients shared the same pattern of dysfunctional word-list learning and word-list recall resembling a hippocampus-dependent memory dysfunction. Furthermore, both patient groups revealed a CSF profile concurring with Alzheimer's disease. However, visuoconstructive capacity, but not figure recall was preserved in AP Aab+ patients, but not in AD Ab-patients with the shared hippocampus-based memory dysfunction. We observed no relevant differences between the AP Aab+ and AD Aab– groups in CSF cell-counts or intrathecal IgG synthesis. The relative frequency of hippocampal and focal atrophy did not differ either between AP Aab+ and AD Aab– groups.DiscussionOur pilot findings are encouraging us to conduct large-scale studies to replicate our discovery of preserved visuoconstruction in AP Aab+ patients with hippocampus-based memory dysfunction. The role of anti-neural autoantibodies is still not fully understood. The detection of these autoantibodies might imply another disease pathology that could be either neuroprotective or be affecting other brain regions, i.e., less pronounced disease activity in the right temporo-parietal regions mainly involved in visuoconstruction.
De la sociedad del riesgo global a la sociedad vulnerable ¿Hacia un análisis sociológico de la pandemia de la COVID-19?
Authors: Morales-Rico Jesús Enrique 
Published: December 2022

El artículo buscó interpretar la pandemia de la COVID-19 a través de la generalización de la vulnerabilidad social, convertida en concepto sociológico, al considerarse como un hecho social provocado por la crisis de la sociedad del trabajo, cuya base fue la gestión de los riesgos y peligros, mediante las instituciones de bienestar estatal, lo que después, en el modelo económico neoliberal, se individualizó, diluyendo la responsabilidad estatal de atención a los peligros derivados de la pandemia, ejerciendo sólo su función de vigilancia y control sobre los individuos de parte de un Estado neoliberal, que dejó de considerarlos como ciudadanos, definidos por los derechos, que fueron protegidos con la existencia del Estado de bienestar.