bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geometri dan Sebaran Perlipatan berdasarkan analisis citra Landsat 9 dan TerraSAR-X Daerah Watuputih dan sekitarnya Kabupaten Rembang
Authors: Faturrakhman Muhammad Luthfi , Sukiyah Emi , Jamal  
Published: May 2023

Cekungan Air Tanah Watuputih secara fisiografis merupakan bagian dari Zona Antiklinorium Rembang. Zona ini Â terdiri dari rangkaian perlipatan en-echelon berarah barat -timur yang terbentuk akibat aktivitas tektonik pada Neogen Akhir. Penelitian terdahulu menyebutkan bahwa struktur geologi memegang peranan penting dalam proses hidrogeologi pada daerah Watuputih. Â Analisis penginderaan jauh dengan menggunakan teknik band composite, image fusion dan principal component analysis dapat membantu dalam mengidentifikasi geometri dan deliniasi lipatan di daerah Watuputih dan sekitarnya. Perlipatan di daerah Watuputih tersusun dari batuan sedimen berumur Neogen dengan kondisi telah mengalami deformasi dan pelapukan yang intensif terutama pada satuan batugamping. Geomorfologi daerah Watuputih menunjukan bentuk jajaran perbukitan dengan arah ketinggian kurang dari 400m dan terdiri dari jajaran perbukitan antiklin dan lembah sinklin bearah relatif barat â€“ timur. Perlipatan pada daerah Watuputih diidentifikasi terbentuk akibat Sesar Rembang-Madura-Kangean-Sakala dengan arah barat daya â€“ timur laut.Kata Kunci Geomorfologi, Lipatan, Rembang, Watuputih, Landsat
Fatores intervenientes experienciados pela população de refugiados no acolhimento do serviço público de saúde: revisão integrativa
Authors: Santos Maria Taís da Silva , Lima Ana Yasmim Gomes de , Silva Maria Fernanda Bandeira da , Andrade Erlaine da Silva , Medeiros Larissa Mercielly Nóbrega , Candeia Victor César Urquiza , Nóbrega Mércia de França , Carvalho Thiozano Afonso de , Pinto Laís Maria Campos , Fernandes Marcelo Costa 
Published: May 2023

O objetivo deste estudo foi analisar os fatores intervenientes experienciados pela população de refugiados no acolhimento ofertado pelo serviço público de saúde. Trata-se de uma revisão integrativa de literatura, em que foi efetuada uma pesquisa eletrônica no Portal de Periódicos CAPES, fazendo o entrecruzamento dos descritores: Refugiados, Direito à Saúde e Assistência à saúde. Após as buscas, encontrou-se 2 611 textos, que com a leitura na íntegra selecionou-se 18 para a amostra final. Foi possível demonstrar as barreiras vivenciadas nos centros de saúde, incluindo a falta de informações sobre os sistemas de saúde, dificuldade na comunicação e compreensão do idioma, divergências culturais, questões legais e de proteção, além da falta de recursos financeiros. Priorizou-se esse campo de investigação, pelo fato de existirem dificuldades e lacunas no conhecimento sobre o acesso à saúde dos refugiados, bem como pelos desafios para a adesão de medidas capazes de garantir uma assistência qualificada.
PERENCANAAN STRATEGIS SISTEM INFORMASI DENGAN METODE WARD AND PEPPARD PADA BISNIS RETAIL (STUDI KASUS : ASIA PLASA TASIKMALAYA)
Authors: Ramdhani Hadianto Harry , Aristi Gea 
Published: May 2023

Asia Plasa merupakan salah satu mall terbesar di Priangan Timur. Sistem informasi yang ada di masing-masing cabang berdiri sendiri-sendiri sehingga para stakeholder tidak bisa dengan mudah melihat perkembangan yang ada. Masih banyak para stakeholder yang belum memahami pentingnya kepatuhan terhadap policy, standard, dan procedure dari suatu proses bisnis. Masalah yang kini dihadapi perusahaan tersebut adalah adanya keraguan dalam pengembangan teknologi informasi. Oleh karena itu perlu dibuat perencanaan strategis sistem informasi yang mengacu dengan strategi bisnis perusahaan. Strategi SI/TI yang selaras dengan strategi bisnis perusahaan dapat meningkatkan efisiensi dan efektifitas dalam mencapai visi dan misi perusahaan. Kelebihan metode Ward and Peppard sendiri dibandingkan dengan metode lain adanya analisis bisnis eksternal dimana memperhitungkan faktor luar perusahaan yang mempengaruhi proses bisnis perusahaan, adanya pemisahaan antara definisi SI dan TI sehingga memudahkan strategi SI/TI untuk dimengerti, Hasil dari penelitian ini adalah telah diketahui sistem informasi yang berjalan di bisnis retail Asia Plasa. Telah dilakukan penerapan metode Ward and Pepard pada perencanaan strategi sistem informasi di Asia Plasa dengan menghasilkan analisis SWOT, strategi SWOT, Mc.Farlan Grid, Analisis PEST, analisis lima persaingan porter, value chain, Critical success factor, portofolio aplikasi Telah dihasilkan portofolio aplikasi yang digunakan untuk masa mendatang sebanyak 24 aplikasi.
Identifying priority sites for the on-farm conservation of landraces and systematic diversity monitoring through an integrated multi-level hotspot analysis: the case of potatoes in Peru
Authors: Dawson Tamsyn , Juarez Henry , Maxted Nigel , de Haan Stef 
Published: May 2023

IntroductionEffective monitoring of the in situ conservation status and change dynamics of landrace populations in their centers of origin ideally requires the identification of sites that are complementary in terms of the richness, uniqueness and coverage of genetic diversity.MethodsWe applied a 4-step approach to identify sites of high potato landrace diversity which will guide the set-up of a network of complementary prospective conservation observatories in Peru, the potato center of origin. A GIS mapping approach was used to determine which combination of sites would provide the most comprehensive and complementary genepool coverage. A landrace inventory was developed from 49 sources, comprising 47,272 landrace records, 97.1% with coordinates, which was used to derive landrace, cultivated species, and cultivar group richness. Data on known indicators of agrobiodiversity, including potato wild relative concurrence, cultivated area, ecogeographic diversity, and ethnolinguistic diversity were included in the spatial overlay analysis, which was used in conjunction with expert opinion data to provide further insight to hotspot selection.ResultsThirteen hotspots with high, unique, and complementary levels of landrace diversity were identified. We recommend that robust baselines are established, documenting current diversity in these sites using semi-standardized methods and metrics for future tracking.DiscussionOur results, while being the most robust of their kind to date, were inevitably affected by data gaps, infrastructure and hotspot biases. New documentation efforts should record landrace diversity in uncovered regions, as well as explore complementary mechanisms to track the conservation status of unique endemic landraces that occur in coldspots.
Single-cell RNA sequencing of peripheral blood reveals immune cell dysfunction in premature ovarian insufficiency
Authors: Zhang Caihong , Yu Dong , Mei Yue , Liu Shanrong , Shao Huijing , Sun Qianqian , Lu Qiong , Hu Jingjing , Gu Hang 
Published: May 2023

BackgroundPremature ovarian insufficiency (POI) is one of the most common causes of female infertility and the etiology is highly heterogeneous. Most cases are idiopathic and the pathogenesis remains unclear. Previous studies proved that the immune system plays a crucial role in POI. However, the precise role of immune system remains unclear. This study aimed to analyze the characteristics of peripheral blood mononuclear cells (PBMC) from patients with POI by single-cell RNA sequencing (scRNA-seq) and to explore the potential involvement of immune response in idiopathic POI.MethodsPBMC was collected from three normal subjects and three patients with POI. PBMC was subjected to scRNA-seq to identify cell clusters and differently expressed genes (DEGs). Enrichment analysis and cell-cell communication analysis were performed to explore the most active biological function in the immune cells of patients with POI.ResultsIn total, 22 cell clusters and 10 cell types were identified in the two groups. Compared with normal subjects, the percentage of classical monocytes and NK cells was decreased, the abundance of plasma B cells was increased, and CD4/CD8 ratio was significantly higher in POI. Furthermore, upregulation of IGKC, IFITM1, CD69, JUND and downregulation of LYZ, GNLY, VCAN, and S100A9 were identified, which were enriched in NK cell-mediated cytotoxicity, antigen processing and presentation, and IL-17 signaling pathway. Among them, IGHM and LYZ were respectively the most significantly upregulated and downregulated genes among all cell clusters of POI. The strength of cell-cell communication differed between the healthy subjects and patients with POI, and multiple signaling pathways were assessed. The TNF pathway was found to be unique in POI with classical monocytes being the major target and source of TNF signaling.ConclusionsDysfunction of cellular immunity is related to idiopathic POI. Monocytes, NK cells, and B cells, and their enriched differential genes may play a role in the development of idiopathic POI. These findings provide novel mechanistic insight for understanding the pathogenesis of POI.
Prognostic significance of thyroid-stimulating hormone receptor antibodies in moderate-to-severe graves’ orbitopathy
Authors: Park Jungyul , Kim Jaehyun , Kim Sang Soo , Choi Hee-Young 
Published: May 2023

DesignRetrospective studyPurposeThe purpose of this retrospective study was to assess the changes in thyroid-stimulating hormone receptor (TSH-R) antibody levels following treatment in patients with moderate-to-severe and active Graves’ orbitopathy (GO) and to investigate the correlation between these antibodies and treatment response.MethodsThe subjects of this study comprised of patients newly diagnosed with moderate-to-severe and active GO within the age range of 19 to 79 years. All participants underwent intravenous methylprednisolone (IVMP) therapy for a duration of 12 weeks. Patients with a clinical activity score (CAS) decrease to or less than 3 and no symptom recurrence for at least 3months after the last dose of IVMP were classified as “Group 1”. Those with a CAS equal to or greater than 4 were classified as “Group 2”. TSH-R antibody levels were measured prior to and following IVMP treatment and treatment response was evaluated after the completion of IVMP therapy. All patients were monitored for a minimum of 6 months post-treatment, with ocular examinations and laboratory tests at the initial visit being included in the analysis.ResultsThe medical records of the 96 patients with GO were retrospectively reviewed. Seventy-five patients (78.1%) were response and 21 (21.9%) were non-responsive to IVMP treatment. A higher TSH-R antibody (TRAb) and thyroid-stimulating antibody (TSAb) following treatment were associated with a high risk of no treatment response (P = 0.017; P = 0.047, respectively). TRAb and TSAb levels before treatment were significantly related to TRAb and TSAb levels after treatment (P &lt; 0.001, respectively). The cut-off values for the prediction of poor treatment response of the TRAb and TSAb before and after treatment were 8.305 IU/L, 5.035 IU/L and 449.5%, 361%, respectively (P = 0.027, P =0.001 and P = 0.136, P = 0.004, respectively).ConclusionIt was observed that elevated levels of TRAb and TSAb prior to IVMP treatment were positively correlated with post-treatment levels of these antibodies. Furthermore, in cases of non-response to IVMP therapy, a diminished decline in both antibodies was observed, and elevated levels of TRAb and TSAb post-treatment were found to be a significant predictor of poor treatment outcome. Measurement of TRAb and TSAb throughout the course of treatment in moderate-to-severe and active cases of GO may offer valuable insights into treatment prognosis and aid in the decision-making process regarding the potential need for increased IVMP dosage or alternative therapeutic strategies.
Identification and analysis of type 2 diabetes-mellitus-associated autophagy-related genes
Authors: Cui Kun , Li Zhizheng 
Published: May 2023

IntroductionAutophagy, an innate safeguard mechanism for protecting the organism against harmful agents, is implicated in the survival of pancreatic â cells and the development of type 2 diabetes mellitus (T2DM). Potential autophagy-related genes (ARGs) may serve as potential biomarkers for T2DM treatment.MethodsThe GSE25724 dataset was downloaded from the Gene Expression Omnibus (GEO) database, and ARGs were obtained from the Human Autophagy Database. The differentially expressed autophagy-related genes (DEARGs) were screened at the intersection of ARGs and differentially expressed genes (DEGs) between T2DM and non-diabetic islet samples, which were subjected to functional enrichment analyses. A protein–protein interaction (PPI) network was constructed to identify hub DEARGs. Expressions of top 10 DEARGs were validated in human pancreatic â-cell line NES2Y and rat pancreatic INS-1 cells using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell viability and insulin secretion were measured after cell transfection with lentiviral vector EIF2AK3 or RB1CC1 into islet cells.ResultsIn total, we discovered 1,270 DEGs (266 upregulated and 1,004 downregulated genes) and 30 DEARGs enriched in autophagy- and mitophagy-related pathways. In addition, we identified GAPDH, ITPR1, EIF2AK3, FOXO3, HSPA5, RB1CC1, LAMP2, GABARAPL2, RAB7A, and WIPI1 genes as the hub ARGs. Next, qRT-PCR analysis revealed that expressions of hub DEARGs were consistent with findings from bioinformatics analysis. EIF2AK3, GABARAPL2, HSPA5, LAMP2, and RB1CC1 were both differentially expressed in the two cell types. Overexpression of EIF2AK3 or RB1CC1 promoted cell viability of islet cells and increased the insulin secretion.DiscussionThis study provides potential biomarkers as therapeutic targets for T2DM.
Higher radiation dose on immune cells is associated with radiation-induced lymphopenia and worse prognosis in patients with locally advanced esophageal squamous cell carcinoma
Authors: Qiu Jianjian , Lin Hancui , Ke Dongmei , Yu Yilin , Xu Jiaying , Qiu Hejin , Zheng Qunhao , Li Hui , Zheng Hongying , Liu Lingyun , Wang Zhiping , Yao Qiwei , Li Jiancheng 
Published: May 2023

BackgroundTo explore the effective dose to immune cells (EDIC) for better prognosis while avoiding radiation-induced lymphopenia (RIL) in patients with locally advanced esophageal squamous cell carcinoma (ESCC).Materials and methodsOverall, 381 patients with locally advanced ESCC receiving definitive radiotherapy with or without chemotherapy (dRT ± CT) between 2014 and 2020 were included in this study. The EDIC model was calculated by radiation fraction number and mean doses to the heart, lung, and integral body. The correlation between EDIC and clinical outcomes was analyzed using Cox proportional hazards regression, and risk factors for RIL were determined by logistic regression analysis.ResultsThe median EDIC was 4.38 Gy. Multivariate analysis revealed that low-EDIC significantly improved the OS of patients when compared with high-EDIC (HR = 1.614, P = 0.003) and PFS (HR = 1.401, P = 0.022). Moreover, high-EDIC was associated with a higher incidence of grade 4 RIL (OR = 2.053, P = 0.007) than low-EDIC. In addition, we identified body mass index (BMI), tumor thickness, and nodal stage as independent prognostic factors of OS and PFS, while BMI (OR = 0.576, P = 0.046) and weight loss (OR = 2.214, P = 0.005) as independent risk factors of grade 4 RIL. In subgroup analyses, the good group had better clinical outcomes than the remaining two groups (P&lt; 0.001).ConclusionThis study demonstrated that EDIC significantly correlates with poor clinical outcomes and severe RIL. Optimizing treatment plans to decrease the radiation doses to immune cells is critical for improving the outcomes.
A triple-drug combination induces apoptosis in cervical cancer-derived cell lines
Authors: Delgado-Waldo Izamary , Contreras-Romero Carlos , Salazar-Aguilar Sandra , Pessoa João , Mitre-Aguilar Irma , García-Castillo Verónica , Pérez-Plasencia Carlos , Jacobo-Herrera Nadia Judith 
Published: May 2023

IntroductionCervical cancer is a worldwide health problem due to the number of deaths caused by this neoplasm. In particular, in 2020, 30,000 deaths of this type of tumor were reported in Latin America. Treatments used to manage patients diagnosed in the early stages have excellent results as measured by different clinical outcomes. Existing first-line treatments are not enough to avoid cancer recurrence, progression, or metastasis in locally advanced and advanced stages. Therefore, there is a need to continue with the proposal of new therapies. Drug repositioning is a strategy to explore known medicines as treatments for other diseases. In this scenario, drugs used in other pathologies that have antitumor activity, such as metformin and sodium oxamate, are analyzed.MethodsIn this research, we combined the drugs metformin and sodium oxamate with doxorubicin (named triple therapy or TT) based on their mechanism of action and previous investigation of our group against three CC cell lines.ResultsThrough flow cytometry, Western blot, and protein microarray experiments, we found TT-induced apoptosis on HeLa, CaSki, and SiHa through the caspase 3 intrinsic pathway, including the critical proapoptotic proteins BAD, BAX, cytochrome-C, and p21. In addition, mTOR and S6K phosphorylated proteins were inhibited in the three cell lines. Also, we show an anti-migratory activity of the TT, suggesting other targets of the drug combination in the late CC stages.DiscussionThese results, together with our former studies, conclude that TT inhibits the mTOR pathway leading to cell death by apoptosis. Our work provides new evidence of TT against cervical cancer as a promising antineoplastic therapy.
No-tillage with straw mulching promotes the utilization of soil nitrogen by rice under wheat–rice and oilseed rape–rice cropping systems
Authors: Yan Fengjun , Zhou Wei , Sun Yongjian , Guo Changchun , Xiang Kaihong , Li Na , Yang Zhiyuan , Wu Yunxia , Zhang Qiao , Sun Yuanyuan , Wang Xiyao , Ma Jun 
Published: May 2023

IntroductionTo investigate the effects of no-tillage with straw mulching on the absorption and utilization of soil nitrogen (N), fertilizer N, and straw N by rice under paddy-upland rotations.MethodsA field experiment with three cropping systems: fallow–rice rotation without straw mulching (FRN), wheat–rice rotation with wheat mulching in rice season (WRS), and oilseed rape–rice rotation with oilseed rape straw mulching in rice season (ORS) was conducted from 2015 to 2017, along with a mini-plot experiment with 15N-labeled urea and straws, which was conducted in 2017.ResultsNo-tillage with straw reduced rice N uptake up to 20 days after transplanting, the total amount of fertilizer N uptake of WRS and ORS rice plants was 46.33 and 61.67 kg/ha, respectively, which was 9.02 and 45.10% higher than that of FRN plants. Soil N was the main source for rice growth, followed by fertilizer N. Soil N uptake by WRS and ORS rice plants was 21.75 and 26.82% higher than that of FRN plants, accounting for 72.37 and 65.47%, respectively, of the total N accumulated in rice plants. Straw mulching increased the N utilization efficiency of tillering, panicle, and total fertilizer by 2.84–25.30%; however, base fertilizer was dependent on straw mulching. The total amount of N released from WRS and ORS straw mulching in the rice season was 34.97 and 24.82 kg/ha, respectively; however, only 3.04 and 4.82% of it was absorbed by the rice plants, accounting for only 0.62 and 0.66% of the total accumulated N.DiscussionNo-tillage with straw mulching under paddy-upland rotations increased the N utilization of rice, especially for the absorption of soil N. These results provide theoretical information for the effective utilization of straw and rational N application practices in rice-based cropping systems.
Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture
Authors: Nowak-Terpiłowska Agnieszka , Zeyland Joanna , Hryhorowicz Magdalena , Śledziński Paweł , Wyganowska Marzena 
Published: May 2023

Phototherapy plays a key role in wound healing and tissue regeneration. The use of lasers has the potential to become an effective and minimally invasive treatment in periodontal and peri-implant disease. The aim of this study was to evaluate the influence of three laser wavelengths with the combination of parameters such as power density and energy density on human gingival fibroblasts (hGFs) in vitro culture. Isolated cells were seeded in 96-well plates with culture medium (DMEM, Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS). After 24 h cells were irradiated (1064, 980 and 635 nm, various energy density value). After 24, 48 and 72 h, cells were evaluated for viability. Data were analyzed by ANOVA followed by Tukey’s HSD test. We found the best outcomes for hGFs irradiated with laser 1064 nm for all combinations of power output (50/400/1000 mW) and energy dose (3/25/64 J/cm2) after 48 h and 72 h compared with control group. Cell viability increase ranged from 0.6× (3 J/cm2, 50 mW) to 1.3× (64 J/cm2, 1000 mW). Our findings indicate that the appropriate use of low-level laser irradiation (LLLI) can increase the proliferation rate of cultured cells. The use of LLLI can be extremely useful in tissue engineering and regenerative medicine.
Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of Ficus religiosa L. with Simultaneous GC-MS Examination
Authors: Kumar Sunil , Arif Muhammad , Kamal Mehnaz , Jawaid Talha , Khan Mohammed Moizuddin , Mukhtar Beenish , Khan Abdullah , Ahmed Saif , AlSanad Saud M. , Al-Khamees Osama A. 
Published: May 2023

Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds’ powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fractions of Ficus religiosa (CFFR and MFFR) were tested for antiplatelet, antithrombotic, thrombolytic, and antioxidant activity in ex vivo mode. The MFFR was particularly investigated for GC-MS and toxicity. The antiplatelet activity of the CFFR, MFFR, and standard drug aspirin at 50 μg/mL was 54.32%, 86.61%, and 87.57%, and a significant delay in clot formation was noted. CFFR at different concentrations did not show a significant effect on the delay of clot formation, antiplatelet, and free radical scavenging activity. The most possible marker compounds for antiplatelet and antioxidant activity identified by GC-MS in the MFFR are salicylate derivatives aromatic compounds such as benzeneacetaldehyde (7), phenylmalonic acid (13), and Salicylic acid (14), as well as Benzamides derivatives such as carbobenzyloxy-dl-norvaline (17), 3-acetoxy-2(1H)-pyridone (16), and 3-benzylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (35). A toxicity study of MFFR did not show any physical indications of toxicity and mortality up to 1500 mg/kg body weight and nontoxic up to 1000 mg/kg, which is promising for the treatment of atherothrombotic diseases.