bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective randomized comparison of three‐dimensional (3D) versus conventional laparoscopy in total colectomy for ulcerative colitis
Authors: Sapci Ipek , GamalEldin Maysoon , Rencuzogullari Ahmet , Yilmaz Sumeyye , Kessler Hermann , Hull Tracy , Delaney Conor P. , Steele Scott R. , Gorgun Emre 
Published: March 2023

AbstractBackground3D laparoscopy has been proposed with the aim of improving the depth perception and overall operative performance. To aim of this study is to compare 3D laparoscopy with conventional 2D laparoscopy in terms of operative time and visual parameters.MethodsThis is a prospective, randomized, single‐center trial designed to determine 10% reduction in the mean operative time. Ulcerative colitis patients >18 years of age who underwent laparoscopic total abdominal colectomy with end ileostomy between 2015 and 2020 were included. Patients were randomized into 3D and 2D laparoscopy groups. Duration of operation and surgeons' evaluation of the visualization system were the primary outcomes.ResultsFifty‐three subjects (26 in 2D, 27 in 3D group) were included in the analysis, with 56% being male. Mean age and body mass index were 40 (16.3) years and 23.5 (4.7) kg/m2, respectively. Twenty‐five subjects underwent single port laparoscopic surgery, of whom 13 were in 3D and 12 in 2D group. Mean operative time was 75.3 (30.8) versus 82.7 (38.6) minutes (P = 0.4) for 3D and 2D groups, respectively. Operative times spent for individual steps were comparable. Post‐operative minor complications (8 in 3D versus 8 in 2D, P = 1) and median number of times for scope maintenance were also similar between the groups. 69% of the visual evaluation survey results favoured 3D over 2D (P = 0.014).ConclusionThree‐dimensional laparoscopy for total colectomy in ulcerative colitis patients is safe and feasible option providing better visualization with no difference in operative time.
New contributions on chronic low back pain: disc infection or contaminated cultures?
Authors: Victoria Monge‐García , Gimeno‐Gascón Adelina , Ventero Maria Paz , Climent‐Barberá Jose M. , Cholbi‐Llobell Francisco , Rodríguez Juan Carlos , Baño‐Ruiz Elena , Caminero‐Canas Miguel Angel , Reus‐Bañuls Sergio 
Published: March 2023

There is controversy about the likely infectious origin of chronic low back pain, because it has been suggested the possibility of a relationship with infection by Cutibacterium acnes (C. acnes). The aim of this study is to compare four methods to determine the presence of a likely infection caused by C. acnes in surgical disc samples. This work is a cross‐sectional observational study in which there are included 23 patients with microdiscectomy indication. Disc samples were taken during surgery and analysis was done by culture, Sanger sequencing, next‐generation sequencing (NGS), and real‐time PCR (qPCR). Furthermore, clinical data collection was conducted, and it was analyzed the presence of the Modic‐like changes on the magnetic resonance imaging. In 5 of the samples from among the 23 patients (21.7%), C. acnes was isolated by culture. However, in none of the samples could its genome be detected through Sanger sequencing, the less sensitive method. Only the qPCR and NGS were able to detect very few copies of the genome of this microorganism in all the samples, with no significant quantitative differences being observed between the patients in whom isolation of the microorganism by culture was evident or not. Furthermore, there were no significant relationships identified between the clinical variables, including Modic alterations and positive cultures. The most sensitive methods to the detect C. acnes were NGS and qPCR. The data obtained do not suggest association between the presence of C. acnes and the clinical process and support the hypothesis that C. acnes is found in these samples only because it is a contamination from the skin microbiome.
Inhibition of oxidative stress by apocynin attenuated chronic obstructive pulmonary disease progression and vascular injury by cigarette smoke exposure
Authors: Chan Stanley M. H. , Brassington Kurt , Almerdasi Suleman Abdullah , Dobric Aleksandar , De Luca Simone N. , Coward‐Smith Madison , Wang Hao , Mou Kevin , Akhtar Alina , Alateeq Rana Abdullah , Wang Wei , Seow Huei Jiunn , Selemidis Stavros , Bozinovski Steven , Vlahos Ross 
Published: March 2023

Background and PurposeCardiovascular disease affects up to half of the patients with chronic obstructive pulmonary disease (COPD), exerting deleterious impact on health outcomes and survivability. Vascular endothelial dysfunction marks the onset of cardiovascular disease. The present study examined the effect of a potent NADPH Oxidase (NOX) inhibitor and free‐radical scavenger, apocynin, on COPD‐related cardiovascular disease.Experimental ApproachMale BALB/c mice were exposed to either room air (Sham) or cigarette smoke (CS) generated from 9 cigarettes·day−1, 5 days a week for up to 24 weeks with or without apocynin treatment (5 mg·kg−1·day−1, intraperitoneal injection).Key ResultsEight‐weeks of apocynin treatment reduced airway neutrophil infiltration (by 42%) and completely preserved endothelial function and endothelial nitric oxide synthase (eNOS) availability against the oxidative insults of cigarette smoke exposure. These preservative effects were maintained up until the 24‐week time point. 24‐week of apocynin treatment markedly reduced airway inflammation (reduced infiltration of macrophage, neutrophil and lymphocyte), lung function decline (hyperinflation) and prevented airway collagen deposition by cigarette smoke exposure.Conclusion and ImplicationsLimiting NOX activity may slow COPD progression and lower cardiovascular disease risk, particularly when signs of oxidative stress become evident.
Understanding the historic legacies of empire from the timbers left behind: Towards critical dendroprovenancing in the British North Atlantic
Authors: Greer Kirsten , Csank Adam , Calvert Kirby , Maddison‐MacFadyen Margot , Smith Andrew , Monk Kimberly , Morrison Sabrina 
Published: March 2023

AbstractThe forests of British North America were integral to Britain's maritime empire. Many of these timbers exist today as wooden beams and flooring at historical dockyards and garrisons such as the Royal Naval Dockyard of Bermuda. In this paper, we investigate what timbers from this Dockyard can tell us about interconnections and empire‐building throughout the North Atlantic region. To do this we drew from approaches of critical physical geography, historical geography, and dendroprovenancing by using timber as a way to interrogate collaboratively the changes in—and connections across—socio‐ecological landscapes. We examined HM Customs records between 1825 and 1850 to determine the flow of timbers to Bermuda. We then sampled timbers from buildings constructed between 1825 and 1853 and analyzed them using isotopic and dendrochronological techniques to establish the probable location of origin of the timber samples. HM Customs records showed that prior to the 1840s, timbers primarily came from British North America, whereas post‐1840 timbers primarily came from the southeastern United States, with some still coming from Europe and British North America. To establish whether timber use by the Royal Navy and Royal Engineers matched the pattern in the customs documents, we looked to the dendrochronological records. Dendrochronologic evidence showed that military construction followed the same pattern, with buildings constructed pre‐1840 using material from British North America and buildings constructed post‐1840 using timbers sourced from the southeastern United States. Finally, using this information we explore some of the connections between regions, in terms of resource use, and the implications of those uses—for example, that the British Admiralty continued to benefit from the practices of slavery through the use of products produced from enslaved labour in other parts of the North Atlantic well after emancipation.
Fusobacterium nucleatum‐induced exosomal HOTTIP promotes gastric cancer progression through the microRNA‐885‐3p/EphB2 axis
Authors: Xin Yiwei , Li Xinyang , Zhang Mengjiao , Shang Ziqi , Luo Zhengdong , Wang Yifeng , Gui Xinru , Liu Qi , Li Tingting , Zeng Shunjie , Schiöth Helgi B. , Zhang Xin , Zhang Yi 
Published: March 2023

AbstractRecent studies have reported that Fusobacterium nucleatum (Fn) is associated with gastric cancer (GC). Cancer‐derived exosomes contain key regulatory noncoding RNAs and are a crucial medium of intercellular communication. However, the function and regulatory mechanism of exosomes (Fn‐GCEx) secreted from Fn‐infected GC cells remains unclear. In this study, Fn‐GCEx enhanced the proliferation, migration, and invasion capacity of GC cells in vitro, as well as tumor growth and metastasis in vivo. HOTTIP was also upregulated in GC cells treated with Fn‐GCEx. Moreover, knockdown of HOTTIP weakened the effects of Fn‐GCEx in recipient GC cells. Mechanistically, HOTTIP promoted EphB2 expression by sponging microRNA (miR)‐885‐3p, thus activating the PI3K/AKT pathway in Fn‐GCEx treated GC cells. Overall, Fn infection induced the upregulation of exosomal HOTTIP from GC cells that subsequently promoted GC progression through the miR‐885‐3p/EphB2/PI3K/AKT axis. Herein, we identify a potential molecular pathway and therapeutic target for GC.
In vivo CRISPR screens identify RhoV as a pro‐metastasis factor of triple‐negative breast cancer
Authors: Jin Ming‐Liang , Gong Yue , Ji Peng , Hu Xin , Shao Zhi‐Ming 
Published: March 2023

AbstractMetastasis is the main death reason for triple‐negative breast cancer (TNBC). Thus, identifying the driver genes associated with metastasis of TNBC is urgently needed. CRISPR screens have dramatically enhanced genome editing and made it possible to identify genes associated with metastasis. In this study, we identified and explored the crucial role of ras homolog family member V (RhoV) in TNBC metastasis. Here, we performed customized in vivo CRISPR screens targeting metastasis‐related genes obtained from transcriptome analysis of TNBC. The regulatory role of RhoV in TNBC was validated using gain‐ or loss‐of‐function studies in vitro and in vivo. We further conducted immunoprecipitation and LC–MS/MS to explore the metastasis mechanism of RhoV. In vivo functional screens identified RhoV as a candidate regulator involved in tumor metastasis. RhoV was frequently upregulated in TNBC and correlated with poor survival. Knockdown of RhoV significantly suppressed cell invasion, migration, and metastasis both in vitro and in vivo. In addition, we provided evidence that p‐EGFR interacted with RhoV to activate the downstream signal pathway of RhoV, thereby promoting tumor metastasis. We further confirmed that this association was dependent on GRB2 through a specific proline‐rich motif in the N‐terminus of RhoV. This mechanism of RhoV is unique, as other Rho family proteins lack the proline‐rich motif in the N‐terminus.
Current understanding and future prospects on Berberine for anticancer therapy
Authors: Goel Anjana 
Published: March 2023

AbstractBerberine (BBR) is a potential plant metabolite and has remarkable anticancer properties. Many kinds of research are being focused on the cytotoxic activity of berberine in in vitro and in vivo studies. A variety of molecular targets which lead to the anticancer effect of berberine ranges from p‐53 activation, Cyclin B expression for arresting cell cycles; protein kinase B (AKT), MAP kinase and IKB kinase for antiproliferative activity; effect on beclin‐1 involved in autophagy; reduced expression of MMP‐9 and MMP‐2 for the inhibition of invasion and metastasis etc. Berberine also interferes with transcription factor‐1 (AP‐1) activity responsible for the expression of oncogenes and neoplastic transformation of the cell. It also leads to the inhibition of various enzymes which are directly or indirectly involved in carcinogenesis like N acetyl transferase, Cyclo‐oxygenase‐2, Telomerase and Topoisomerase. In addition to these actions, Berberine plays a role in, the regulation of reactive oxygen species and inflammatory cytokines in preventing cancer formation. Berberine anticancer properties are demonstrated due to the interaction of berberine with micro‐RNA. The summarized information presented in this review article may help and lead the researchers, scientists/industry persons to use berberine as a promising candidate against cancer.
Radiation of bilateral adrenal metastases is associated with a high risk of primary adrenal insufficiency
Authors: Herndon Justine , Hallemeier Christopher , Jethwa Krishan , Shariff Afreen , Bancos Irina 
Published: March 2023

AbstractBackgroundAdrenal metastasis is the most common adrenal malignancy and can be bilateral in up to 43% of patients. Radiotherapy (RT) is one option available to treat adrenal metastases. The risk of primary adrenal insufficiency (PAI) after adrenal RT is unclear.ObjectiveDetermine the incidence and the timeline of PAI in patients undergoing adrenal RT.Design, Setting and ParticipantsSingle‐centre longitudinal retrospective cohort study of adult patients with adrenal metastases treated with RT between 2010 and 2021.ResultsOf 56 patients with adrenal metastases treated with adrenal RT, eight (14.3%) patients developed PAI at a median of 6.1 months (interquartile range [IQR]: 3.9–13.8) after RT All patients developing PAI had either unilateral RT in the setting of contralateral adrenalectomy or bilateral adrenal RT. Patients who developed PAI received a median RT dose of 50 Gy (IQR: 44–50 Gy), administered in a median of five fractions (IQR: 5–6). Treated metastases decreased in size and/or metabolic activity on positron emission tomography in seven patients (87.5%). Patients were initiated on hydrocortisone (median daily dose of 20 mg, IQR: 18–40) and fludrocortisone (median daily dose of 0.05 mg, IQR: 0.05–0.05 mg). At the end of the study period, five patients died, all due to extra‐adrenal malignancy, at a median time of 19.7 months (IQR: 16–21.1 months) since RT and median time of 7.7 months (IQR: 2.9–12.5 months) since the diagnosis of PAI.ConclusionPatients receiving unilateral adrenal RT with two intact adrenal glands have a low risk of PAI. Patients receiving bilateral adrenal RT have a high risk of PAI and require close monitoring.
1051.Cathaya argyrophyllaChun & Kuang: Pinaceae
Authors: Christenhusz Maarten J. M. , Battle Christine 
Published: March 2023

SummaryThe ‘pearl of the forest’, also known as ‘yin shan’ or the ‘giant panda of the plant kingdom’,Cathaya argyrophyllais illustrated here (t. 1051) from a tree cultivated at the Royal Botanic Gardens, Kew. The discovery and phylogenetics of this species are discussed, and its introduction into horticulture is presented. A detailed description is provided here, and ecology, cytology, etymology and conservation are discussed. Advice on cultivation is also given.
1057.Heptacodium miconioidesRehder: Caprifoliaceae
Authors: Christenhusz Maarten J. M. , Mian Sahr , Lambkin Deborah 
Published: March 2023

SummaryHeptacodium miconioides,also known as ‘the seven‐son flower’, is illustrated from a plant cultivated at the Royal Botanic Gardens, Kew. Originally from China, it was discovered in the early 20thCentury, but was first introduced into western horticulture in the 1980s from plants grown at the Hangzhou Botanical Garden. A detailed description is provided here, and the ecology, cytology and etymology of the species are discussed. Type specimens have been studied and lectotypes are designated. This attractive hardy, autumn‐flowering shrub is a great addition to any woodland garden and should be much more commonly grown.
Theorizing Power in Community Economies: A Women's Cooperative in Northern Kurdistan
Authors: Turker Kaner Atakan 
Published: March 2023

ABSTRACTUnder the Democratic Autonomy project, Turkey's Kurdish Movement has pursued self‐governance since the mid‐2000s and promoted cooperatives and communal modes of production across Northern Kurdistan. Drawing upon the engagement of diverse and community economies studies with assemblage thinking, this article utilizes assemblage thinking to expand our understanding of power dynamics in community economies, and to reveal the power‐led processes involved in building and maintaining community economies. The article focuses on the case of a women's cooperative spearheaded by the Kurdish Movement, which managed to circumvent state oppression and stay in business, despite the turmoil that erupted in Northern Kurdistan after the collapse of peace negotiations between the Turkish state and the Kurdish Movement in 2015.
Diffuse reflectance spectroscopy (DRS) for rapid soil testing and soil quality assessment in smallholder farms
Authors: Majeed Israr , Garg Kaushal K. , Venkataradha A. , Purushothaman Naveen K. , Roy Sourav , Reddy Nagarjuna N. , Singh Ramesh , Anantha K. H. , Dixit Sreenath , Das Bhabani S. 
Published: March 2023

AbstractRapid soil testing and soil quality assessment are essential to address soil degradation and low farm incomes in smallholder farms. With the objective of testing diffuse reflectance spectroscopy (DRS) to rapidly assess soil chemical properties, nutrient content and a soil quality index (SQI), samples of surface soil were collected from 1113 smallholder farms in seven districts in Bundelkhand region of Uttar Pradesh, India. A minimum dataset (MDS) approach was followed to estimate SQI using the three chemical parameters of soil pH, electrical conductivity (EC) and soil organic carbon (SOC), and 11 different soil nutrients. Principal component and correlation analyses showed that soil pH, SOC content and three available nutrients − copper (Cu), iron (Fe) and sulphur (S) − may constitute the MDS. Estimated SQI values showed strong positive correlation with crop yields. Results of chemometric modelling showed that the DRS approach could yield the coefficient of determination (R2) values in the validation datasets ranging from 0.79 to 0.94 for exchangeable calcium (Ca) followed by 0.67–0.88 for exchangeable potassium (K), 0.52–0.86 for SOC and 0.53–0.81 for available boron (B) content. Except in one district, the DRS approach could be used to estimate SQI values with R2 values in the range of 0.63–0.81; an R2 value of 0.71 was obtained in the pooled dataset. We also estimated the three‐tier soil test crop response (STCR) ratings to compare DRS and wet chemistry soil testing approaches. Similar STCR ratings were obtained for both these approaches in more than 86% of the samples. Parameters for which both the methods yielded similar ratings in more than 80% of the samples were EC (>98%), pH and exchangeable Ca (>81%) and available B (>89%). With similar ratings, these results suggest that the DRS approach may safely be used for farmers' fields, replacing the traditional wet analysis approach of soil testing.
Incidence and outcomes of head injuries in people with and without Parkinson disease
Authors: Ilmaniemi Sarianna , Tolppanen Anna‐Maija , Herukka Sanna‐Kaisa , Hartikainen Päivi , Hartikainen Sirpa 
Published: March 2023

AbstractBackground and purposeFall‐related injuries are a major health concern among people with Parkinson disease (PD). We compared the incidence and postinjury mortality of head injuries and traumatic brain injury (TBI) among persons with and without PD.MethodsThis register‐based study was conducted on the FINPARK cohort, which includes 22,189 persons who were diagnosed with PD in Finland during 1996–2015. We excluded persons with a previous head injury, leaving 20,514 persons with PD. For each person with PD, 1–7 matching persons without PD and previous head injury were identified with respect to age, sex, and residence. The Cox proportional hazard model was used to estimate hazard ratios for head injury. A logistic regression model was used to compare mortality.ResultsPersons with PD had 2.16‐fold (95% confidence interval [CI] = 2.06–2.26) risk of all head injuries and 1.97‐fold (95% CI = 1.84–2.10) risk of TBI after adjustment for age, sex, and comorbidities. Persons with PD had higher 1‐year mortality after any type of head injury (adjusted odds ratio [aOR] = 1.44, 95% CI = 1.28–1.62), TBI (aOR = 1.33, 95% CI = 1.14–1.57), or non‐TBI head injury (aOR = 1.72, 95% CI = 1.42–2.07) than persons without PD. The higher risk of mortality was observed 6 months after TBI and 1 month after non‐TBI injury in persons with PD. Persons with PD and head injury also had higher 1‐year mortality than persons with PD and without head injury.ConclusionsPersons with PD have a higher risk of head injury and higher postinjury mortality than persons without PD.
Factors associated with physical activity in young people with haemophilia A on prophylaxis
Authors: Matlary Ruth Elise D. , Grydeland May , Glosli Heidi , Rueegg Corina Silvia , Holme Pål André 
Published: March 2023

AbstractIntroductionPhysical activity (PA) is influenced by numerous factors, and the literature describing why people with haemophilia (PWH) are physically active or not is inconclusive.AimsTo investigate factors associated with PA (mean min/day in light (LPA), moderate (MPA), vigorous (VPA) and total PA, and proportion meeting World Health Organization (WHO) weekly moderate‐to‐vigorous (MVPA) recommendations) among young PWH A.MethodsForty PWH A on prophylaxis from the HemFitbit study were included. PA was measured using Fitbit devices and participant characteristics were collected. Potential factors associated with PA were investigated by univariable linear regression models for continuous PA outcomes, and descriptively for teenagers meeting/not meeting WHO MVPA recommendations only, because all except one adult met PA recommendations.ResultsMean age (n = 40) was 19.5 years (SD 5.7). Annual bleeding rate was nearly zero and joint scores were low. We found an increase of four min/day in LPA (95% confidence interval (CI) 1–7) per year increase in age. Participants with ‘Haemophilia Early Arthropathy Detection with Ultrasound’ (HEAD‐US) score ≥1 engaged in mean 14 min/day less MPA (95% CI −23.2 to −3.8), and 8 min less VPA (95% CI −15.0 to −0.4) compared to participants with HEAD‐US score 0. Teenagers who met PA recommendations had slightly better joint status compared to those who did not meet recommendations.ConclusionThese findings indicate that presence of mild arthropathy does not affect LPA but may have a negative impact on PA of higher intensities. Early start of prophylaxis may be an important determinant of PA.
Ferry quarantine: The pandemic as a pretext to deny healthcare access to undocumented migrants and asylum seekers in Italy
Authors: Botrugno Carlo 
Published: March 2023

AbstractThe outbreak of COVID‐19 forced national governments and health authorities worldwide to adopt measures aimed at protecting population health, most of which resulted in prolonged restrictions to individual freedom(s) and fundamental rights. In some cases, the pandemic represented a pretext for macroscopic and unjustified human rights violations, as in the case of the “ferry quarantine,” that is the use of commercial boats by the Italian Government to forcibly isolate migrants and asylum seekers from the rest of the population. Such a measure does not have any rationale at the epidemiological level and poses serious obstacles to the provision of adequate care to vulnerable people such as migrants and asylum seekers. The pandemic has been used as a pretext to implement discriminatory and disproportionate measures whose effect has been denying healthcare access to undocumented migrants and asylum seekers.