bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Total Intravenous Versus Inhalational Anesthesia on Bleeding During Endoscopic Ear Surgery: Preliminary Results From a Case-Control Study
Authors: Molinari Giulia , Reale Marella , Alicandri-Ciufelli Matteo , Villari Domenico , Presutti Livio , Feminò Raimondo , Fernandez Ignacio Javier , Lucidi Daniela 
Published: May 2023

Objective
To assess the impact of the type of maintenance anesthesia on the bleeding conditions of the surgical field and hemodynamic parameters during endoscopic ear surgery (EES), comparing totally intravenous inhalational anesthesia (TIVA) with inhalational anesthesia (IA).

Study Design
Retrospective case-control study.

Setting
Tertiary referral center.

Patients
Fifteen consecutive EES cases performed with TIVA between 2019 and 2020 at our Institution were matched to a control group of patients who underwent EES with IA in the same period. Patients with American Society of Anesthesiologists IV grade, acute otitis before surgery, congenital or acquired coagulopathies, use of anti-inflammatory drugs, or antiaggregant or anticoagulant therapy in the 5 days before the intervention were excluded.

Interventions
Both primary and revision tympanoplasty and stapes surgery were considered.

Main Outcome Measures
Surgical videos were reviewed to quantify the entity of bleeding according to the Modena Bleeding Score. Hemodynamic parameters during surgery were retrospectively collected. A comparison between the two groups was performed.

Results
No statistically significant differences between the two groups in terms of bleeding were found at any of time point evaluated. The tympanomeatal flap elevation resulted in the bloodiest step in both groups. Hemodynamic parameters were similar in both groups (p > 0.05).

Conclusions
These preliminary data do not support a significant difference in bleeding conditions and hemodynamic parameters between EES patients receiving TIVA and those receiving IA. Further studies involving a higher number of patients will improve our understanding on how maintenance anesthesia with TIVA may be beneficial in terms of bleeding control as compared with IA for patients undergoing EES.
Lyrical Trends: An Analysis of Music’s (Mis) Use of Vertigo
Authors: Jiam Nicole T. , Chern Alexander , Watson Jowan , Naples James G. 
Published: May 2023

Background
Popular culture and music have long served as windows into the common attitudes, trends, and issues of the time. The representation of vertigo in music has not been previously explored. The objective of this study was to analyze the portrayal of vertigo in music through content and sentiment analysis of song lyrics.

Methods
A large song lyric database was queried to identify English language song titles containing the word “vertigo.” A sentiment and content analysis of the lyrics was performed to delineate the most frequently used words, the accuracy of vertigo depictions, and emotional valence (i.e., the ratio of positive to negative references to vertigo).

Results
Between 1969 and 2022, there were a total of 54 songs specifically titled “Vertigo.” Most songs (73%) portrayed vertigo negatively, and the prevalence of negative sentiment within music increased with each decade. The three most common words being used in association with vertigo within music were “feel” (n = 97), “love” (n = 66), and “falling” (n = 57). In early decades, songs using the word vertigo misrepresented the condition and associated perceptions relative to common medical understanding. In more recent decades, vertigo was used in a way more representative of medical interpretations.

Conclusion
The depiction of vertigo in songs has changed over time, and more recently, usage has reflected Bárány Society definitions. Interestingly, the negative sentiment has also increased with time. This work provides a lyrical analysis of vertigo that may improve physician understanding of the cultural usage of this challenging symptom.
Trends in patient’s use of sexual health services during COVID-19 in a network of STD clinics, STD Surveillance Network, 2019- 2021
Authors: Llata Eloisa , Schumacher Christina , Grigorov Irina , Danforth Brandi , Pathela Preeti , Asbel Lenore , Nguyen Trang Quyen , Berzkalns Anna , Kreisel Kristen M. 
Published: May 2023

ABSTRACT

Background
The initial years of the COVID-19 pandemic disrupted sexual healthcare clinic’s services. We describe use patterns by patient characteristics, and the use of telehealth (TH) services among a network of sexually transmitted disease (STD) clinics.

Methods
Data were collected using a survey to assess the impact of COVID-19 from March – December 2020 among seven jurisdictions who contribute STD visit-level data as part of the STD Surveillance Network. As a complement to the survey, retrospective data from January 2019 – December 2021 from these seven STD clinics in the same seven jurisdictions were examined for monthly utilization trends by overall visits, patient characteristics, and TH visits.

Results
Survey results indicated seven clinics prioritized patients for in-person visits and four jurisdictions reported urgent care centers were the most common referral location. In April 2020 (relative to April 2019) clinic visits and unique patients decreased by 68.0% and 75.8%, respectively. TH were documented in four clinics, beginning in March 2020, peaking in December 2020, and tapering until December 2021. We observed the number of clinic visits (-12.2%) and unique patients presenting for care (-27.2%)in December 2021 had yet to return to levels to that seen in December 2019.

Conclusion
STD clinics showed fragility and resiliency in their adjustment to the pandemic; allowing for the continuation of services. Overall patient census has been slow to return to pre-pandemic levels, and many patients may still not be seeking timely care. This could result in missed opportunities to screen and treat STIs and increasing the possibility of harmful sequelae.
Examining the Patterns of Mpox Vaccine Uptake in a Vulnerable Population
Authors: Zucker Roy , Wolff-Sagy Yael , Ramot Noga , Raviv Yuval , Lavie Gil , Netzer Doron , Hammerman Ariel , Arbel Ronen 
Published: May 2023

Abstract

Background
The current Mpox outbreak presents unique vaccination challenges in vulnerable populations. Understanding factors associated with vaccine uptake in vulnerable populations is required for a successful vaccination campaign.

Methods
This population-based cohort study was conducted in Clalit Health Services (CHS) and included all individuals eligible for the MVA vaccine. Cox proportional hazards models were used to assess the characteristics associated with uptake of the first vaccine dose.

Results
Attendance to a primary healthcare clinic in the Tel Aviv district, repeated STI screening, and the recent purchase of HIV-PrEP or PDE5 inhibitors were associated with higher vaccine adherence, while previous non-adherence with recommended vaccines, low sociodemographic status, and history of HIV were associated with lower adherence.

Conclusions
These findings highlight the need for proactive patient and healthcare provider-oriented educational campaigns to curb vaccine hesitancy, and may help direct resources toward underserved populations, hence increasing equality in vaccine enrollment.
Complications of Long-Acting Reversible Contraceptive Modalities
Authors: Irgens-Moller Nicole , Baum Carl R. 
Published: May 2023

Abstract
Long-acting reversible contraception has risen in popularity in recent decades and is becoming one of the more common modalities of contraception in the United States, with a growing adolescent population among its users. The 2 modalities of long-acting reversible contraception—intrauterine devices and subcutaneous hormonal implants/etonogestrel implants (referred to as Nexplanon in the US)—are beneficial for their excellent effectiveness, ease of use, and safety profile. This article reviews the pharmacology of these modalities, common complications and their presentations, and the initial evaluation and management in the pediatric emergency department setting. Where applicable, attention will be paid to problems unique to the adolescent population.
Double-Reversed Costal Cartilage Graft for Nasal Reconstruction
Authors: Kalmar Christopher L. , Carlson Anna R. , Bartlett Scott P. 
Published: May 2023

Summary:
Costal cartilage provides the ideal graft for reconstruction of the nasal dorsum and tip because of its abundant supply and flexural strength. Nevertheless, autologous costal cartilage grafts can experience warping. Several techniques have been devised to offset the intrinsic warping effects of cartilage, but current techniques have several disadvantages. The authors present a multimedia demonstration of the double-reversed technique for costal cartilage reconstruction. This strategy is unique in its dual axis of rotation to offset warp in all dimensions. This technique can be performed with conventional instruments, does not introduce foreign material, and provides robust support to counteract the often scarred and contracted soft-tissue envelope.
Effects of changing the COS protocol in patients without a transplantable embryo in the previous IVF/ICSI cycle
Authors: Yi Shan-Jia , Yang Yi-Hua , Bi Yin , Zeng Zhong-Hong , Wang Xi , Li Mu-Jun , Ma Wen-Hong 
Published: May 2023

Objective:
Some patients fail to obtain an embryo for transplantation during previous in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles, and require multiple reproductive treatments. This study aimed to evaluate whether changing the control ovarian stimulation (COS) protocol during the subsequent stimulation cycle could improve laboratory and clinical outcomes in these patients.

Methods:
Patients without a transplantable embryo (TE) in the previous IVF/ICSI cycles were recruited during their second cycles. They were classified into two groups according to their first cycle protocol: Group A, patients treated with a gonadotropin-releasing hormone agonist (GnRH-a), and Group B, patients treated with a gonadotropin-releasing hormone antagonist (GnRH-ant). The study group included patients whose stimulation protocols were changed, whereas the control group consisted of patients who used the same stimulation protocol in the second cycle. We then compared the numbers of oocytes collected (OC) and TE, the incidence of non-TE, the pregnancy rate (PR), and the live birth rate (LBR).

Results:
In Group A, the numbers of OC and TE were significantly lower (6.0±4.7 vs. 9.4±6.4, 2.±2.2 vs. 4.5±3.8, P<0.05) in the study group compared with those in the control group. In Group B, the numbers of OC and TE were higher (7.0±5.5 vs. 4.0±4.3, 3.5±3.4 vs. 1.8±2.1, P<0.05) in the study group. There was a significant increase in the incidence of non-TE (AOR=2.12, 95% CI:1.04–4.69) of the study group in Group A but not in Group B. No significant differences in the PR or LBR were found between the study and control groups in either Group A or B.

Conclusions:
Changing the COS protocol from GnRH-ant to GnRH-a or continuing the GnRH-a protocol can improve laboratory outcomes in patients with no TE in the previous IVF/ICSI cycle.
Sphingomonas lycopersici sp. nov., isolated from tomato rhizosphere soil
Authors: Gao Ruixiang , Dong Honghong , Liu Yang , Yao Qing , Li Huaping , Zhu Honghui 
Published: May 2023

Two aerobic, Gram-stain-negative, non-motile and non-spore-forming rods bacterial strains, designated MMSM20T and MMSM24, were isolated from tomato rhizosphere soil and could produce indole-3-acetic acid and siderophore. Phylogenetic analyses based on 16S rRNA gene sequences and 92 core genes showed that strains MMSM20T and MMSM24 belonged to the genus

Sphingomonas

and were most closely related to three validly published species

Sphingomonas jeddahensis

G39T,

Sphingomonas mucosissima

DSM 17494T and

Sphingomonas dokdonensis

DSM 21029T. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strains MMSM20T and MMSM24 were 97.6 and 81.0 %, respectively, demonstrating that they were conspecific. The ANI and dDDH values between the two strains and the three type strains above were below the threshold values for species delimitation. The genomic DNA G+C contents of strains MMSM20T and MMSM24 were 66.6 and 66.4 mol%, respectively. The major fatty acids of the two strains were identified as C14 : 0 2OH, summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c); the predominant quinone was ubiquinone 10; the polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid and unidentified lipids. Results of phenotypic and genotypic analyses supported that strains MMSM20T and MMSM24 represent a novel species of the genus

Sphingomonas

, for which the name Sphingomonas lycopersici sp. nov. is proposed. The type strain is MMSM20T (=GDMCC 1.3401T=JCM 35647T).
Societal feedback induces complex and chaotic dynamics in endemic infectious diseases
Authors: Wagner Joel , Bauer Simon , Contreras Sebastian , Fleddermann Luk , Parlitz Ulrich , Priesemann Viola 
Published: May 2023

AbstractClassically, endemic diseases are expected to display relatively stable, predictable infection dynamics. Indeed, diseases like influenza show yearly recurring infection waves that can be anticipated accurately enough to develop and distribute new vaccines. In contrast, newly-emerging diseases may cause more complex, unpredictable dynamics, like COVID-19 has demonstrated. Here we show that complex infection dynamics can also occur in the endemic state of seasonal diseases when including human behaviour. We implement human behaviour as a feedback between incidence and disease mitigation and study the system as anepidemiological oscillatordriven by seasonality. When behaviour and seasonality have a comparable impact, we find a rich structure in parameter and state space with Arnold tongues, co-existing attractors, and chaos. Moreover, we demonstrate that if a disease requires active mitigation, balancing costs of mitigation and infections can lead societies right into this complex regime. We observe indications of this when comparing past COVID-19 and influenza data to model simulations. Our results challenge the intuition that endemicity implies predictability and seasonal waves, and show that complex dynamics can dominate even in the endemic phase.
Interplay between charge distribution and DNA in shaping HP1 paralog phase separation and localization
Authors: Phan Tien M. , Kim Young C. , Debelouchina Galia T. , Mittal Jeetain 
Published: May 2023

AbstractThe heterochromatin protein 1 (HP1) family is a crucial component of heterochromatin with diverse functions in gene regulation, cell cycle control, and cell differentiation. In humans, there are three paralogs, HP1α, HP1β, and HP1γ, which exhibit remarkable similarities in their domain architecture and sequence properties. Nevertheless, these paralogs display distinct behaviors in liquid-liquid phase separation (LLPS), a process linked to heterochromatin formation. Here, we employ a coarse-grained simulation framework to uncover the sequence features responsible for the observed differences in LLPS. We highlight the significance of the net charge and charge patterning along the sequence in governing paralog LLPS propensities. We also show that both highly conserved folded and less-conserved disordered domains contribute to the observed differences. Furthermore, we explore the potential co-localization of different HP1 paralogs in multicomponent assemblies and the impact of DNA on this process. Importantly, our study reveals that DNA can significantly reshape the stability of a minimal condensate formed by HP1 paralogs due to competitive interactions of HP1α with HP1β and HP1γ versus DNA. In conclusion, our work highlights the physicochemical nature of interactions that govern the distinct phase-separation behaviors of HP1 paralogs and provides a molecular framework for understanding their role in chromatin organization.Graphical abstract
Introduction of a Penicillin Allergy De-Labelling Program with Direct Oral Challenge is Associated with Increased Downstream Utilization of Beta-Lactam Antimicrobials: A Multicenter Parallel Cohort Study with Crossover Evaluation
Authors: Mir Adhora , Lanoue Derek , Zanichelli Veronica , van Walraven Carl , Olynych Timothy , Nott Caroline , MacFadden Derek 
Published: May 2023

ABSTRACTBackgroundPenicillin allergy labels are common and are often inaccurate. These labels can lead to unnecessary use of second-line non-beta-lactam antibiotics, and worse clinical outcomes.ObjectivesWe measured the impact of the introducing of a standardized proactive penicillin allergy de-labelling program with oral amoxicillin challenge on subsequent antibiotic use.MethodsWe performed a retrospective comparison of parallel cohorts from two separate tertiary care hospital campuses across two penicillin de-labelling intervention periods. Outcomes included data including penicillin allergy label and antibiotic use, were collected for the index admission and the subsequent 6-month period. Descriptive statistics as well as multivariate regression analyses were performed.ResultsA total of 368 patients with penicillin allergy label were included across two campuses and study periods. 24 (13.8%) patients in the intervention group sustained penicillin allergy label at 30 days from admission vs. 3 (1.5%) in the non-intervention group (p < 0.001). In the 6-months following admission, beta-lactams were prescribed more frequently in the intervention groups vs. the non-intervention groups for all patients (28 [16.1%] vs 15 [7.7%], p= 0.04) and for only those patients who received antibiotics (28/46 [60.9%] vs. 15/40 [37.5%], p=0.097). In a multivariate analysis, the intervention was found to be associated with an increased odds of beta-lactam prescribing in all patients (OR 2.49, 95% CU 1.29-5.02) and in those prescribed at least one antibiotic (OR 2.44, 95% CI 1.00-6.15). There were no differences in overall antibiotic prescribing by intervention and non-intervention group during admission (113 [64.9%] vs. 112 [57.7%]) or within 6-months (46 [26.4%] vs. 40 [20.6%]). No drug related adverse events were reported.ConclusionsProactive penicillin allergy de-labelling for inpatients was associated with a reduced number of penicillin allergy labels and increased utilization of beta-lactam vs. other antibiotics in the subsequent 6-months.Capsule SummaryA proactive systematic approach to antibiotic allergy de-labelling for inpatients with penicillin allergy label results in an increased number of patients de-labelled at hospital discharge and increased beta-lactam use in the subsequent 6 months.
In vivoProtein Footprinting Reveals the Dynamic Conformational Changes of Proteome of Multiple Tissues in Progressing Alzheimer’s Disease
Authors: Son Ahrum , Kim Hyunsoo , Diedrich Jolene K. , Bamberger Casimir , McClatchy Daniel B. , Yates John R. 
Published: May 2023

AbstractNumerous studies have investigated changes in protein expression at the system level using proteomic mass spectrometry, but only recently have studies explored the structure of proteins at the proteome level. We developed covalent protein painting (CPP), a protein footprinting method that quantitatively labels exposed lysine, and have now extended the method to whole intact animals to measure surface accessibility as a surrogate of in vivo protein conformations. We investigated how protein structure and protein expression change as Alzheimer’s disease (AD) progresses by conducting in vivo whole animal labeling of AD mice. This allowed us to analyze broadly protein accessibility in various organs over the course of AD. We observed that structural changes of proteins related to ‘energy generation,’ ‘carbon metabolism,’ and ‘metal ion homeostasis’ preceded expression changes in the brain. We found that proteins in certain pathways undergoing structural changes were significantly co-regulated in the brain, kidney, muscle, and spleen.
Similarity index of ex-Gaussian reaction time signatures
Authors: Manjarrez Elias , DeLuna-Castruita Angel , Lizarraga-Cortes Victoria , Flores Amira 
Published: May 2023

AbstractIn psychology and cognitive neuroscience, reaction time (RT) series and their ex-Gaussian distributions are commonly used to explore the time course of cognitive processes. This study investigated the hypothesis that successive triads of ex-Gaussian mu, sigma, and tau parameters of reaction-time variability across time can be used to construct a geometrical object we termed ex-Gaussian RT-signature, which could help characterize interindividual differences between congruent and incongruent stimuli. To test this hypothesis, we calculated the similarity index of these geometrical objects in young adult participants without detectable neurological disorders. Our findings show that each participant displayed distinct ex-Gaussian RT-signatures in a Cartesian 3D plot, thus exhibiting distinct psychophysical fingerprints. Furthermore, our results revealed that the ex-Gaussian RT-signatures for incongruent stimuli demonstrated a significantly higher similarity index across participants than congruent stimuli (p<0.001; Cohen d=0.4). We suggest that visualizing this psychophysical signature could serve as a valuable tool in characterizing differences in cognitive functioning between individuals, thus providing insights into the development of personalized medicine.
Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions
Authors: Tsagiopoulou Maria , Rashmi Sonal , Aguilar Sergio , Nieto Juan , Gut Ivo G. 
Published: May 2023

AbstractDespite the large number of publicly available single-cell datasets, there is a limited understanding of the distinct resident immune cells and their concomitant features in diverse human organs. To address this, we compiled a dataset of 114,275CD45+immune cells from 14 organs from healthy donors. Although the transcriptome of immune cells is constant across organs, organ-specific gene expression changes were detected revealing unique expression in certain organs (GTPX3in kidney,DNTTandACVR2Bin thymus). These alterations are associated with different transcriptional factor activities and pathways including metabolism.TNF-αsignaling through theNFkBpathway was found in various organs and immune compartments including distinct expression profiles ofNFkBfamily genes and their target genes such as cytokines indicating their role in cell positioning. Taken together, immune cells not only protect the organs but also adapt to the host organ environment and contribute to its function and homeostasis.