bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolutionary patterns and research frontiers in autophagy in podocytopathies: a bibliometric analysis
Authors: Liu Feng , Xu Ziyu , Chen Gaijie , Xu Xiaojing , Cao Huixia , Chen Jiefang 
Published: January 2025

IntroductionPodocytopathies are a uniquely renal disease syndrome, in which direct or indirect podocyte injury leads to proteinuria or nephrotic syndrome. Of the many factors that contribute to podocytopathies, the abnormal regulation of autophagy, such insufficient or excessive autophagy levels, have been proposed to play a significant role in the occurrence and development of podocytopathies. However, there still has been a lack of systematic and comparative research to elucidate exact role of autophagy in podocytopathies and its current research status. This study aims to utilize bibliometric analysis to clarify the role of autophagy in the pathogenesis of podocytopathies, analyze the research focus in this area, as well as explore the future research trends.MethodsWe retrieved original articles and review papers with respect to autophagy in podocytopathies research published between the year 2008 and 2022 from the Web of Science Core Collection (WOSCC). Then, VOSviewer and CiteSpace software were employed to reveal the leading subjects and generate visual maps of countries/regions, organizations, authors, journals, and keyword networks in this field.Results and discussionA total of 825 publications regarding autophagy in podocytopathies published between 2008 and 2022 were included, with China contributing the most followed by the United States and Japan. Professor Koya Daisuke, Professor He Qiang, and Professor Jin Juan are the most prolific researchers in this field. Oxidative stress, the NLRP3 inflammasome, and therapeutic targets were the knowledge base for the research in this special field. Taken together, this bibliometric analysis helps us reveal the current research hotspots and guide future research directions, which provides a reference for scholars to further investigate the role of autophagy in podocytopathies as well as conduct clinical trial with autophagy regulators in podocytopathies.
Comparison of an AI-based mobile pupillometry system and NPi-200 for pupillary light reflex and correlation with glaucoma-related markers
Authors: Jaworski Damian , Suwała Karolina , Kaluzny Bartlomiej J , Kaluzny Jakub J. 
Published: January 2025

IntroductionGlaucoma is a leading cause of blindness, often progressing asymptomatically until significant vision loss occurs. Early detection is crucial for preventing irreversible damage. The pupillary light reflex (PLR) has proven useful in glaucoma diagnosis, and mobile technologies like the AI-based smartphone pupillometer (AI Pupillometer) offer a promising solution for accessible screening. This study assesses the reliability of the AI Pupillometer in detecting glaucoma.MethodsIn Experiment 1, 20 healthy participants were assessed using both the AI Pupillometer and the NPi-200 device to evaluate equivalence in measuring PLR. Each eye underwent three trials. Experiment 2 included 46 participants, 24 with primary open-angle glaucoma (POAG) and 22 healthy controls. PLR measurements from the AI Pupillometer were correlated with structural and functional ocular parameters. An additional study expanded the sample to 387 participants (103 glaucoma patients, 284 controls), focusing on differential pupillometry parameters to minimize ambient light interference.ResultsIn Experiment 1, the AI Pupillometer demonstrated strong correlations with the NPi-200 in key parameters like initial pupil size (r = 0.700), constricted pupil size (r = 0.755), and constriction velocity (r = 0.541), confirming its reliability. In Experiment 2, although no statistically significant differences in light-corrected PLR parameters were found between groups, glaucoma patients had a marginally higher constricted pupil size (p = 0.1632). Significant correlations were observed between pupillometry and advanced ocular imaging results, notably between constriction amplitude and visual field loss. The additional study revealed significant differences in constriction amplitude (p = 0.014) and relative pupil size change (p = 0.0072) between glaucoma patients and controls, reinforcing the AI Pupillometer’s diagnostic potential.ConclusionThis study confirms the AI Pupillometer as a reliable, accessible tool for glaucoma screening. Mobile diagnostics could enhance early detection, improving outcomes for glaucoma patients.
Acute beneficial effects of a functional energy shot on cognitive performance and mood states during cognitively demanding task performance: a randomized, double-blind, placebo-controlled, crossover trial
Authors: O’Shea Olivia K. , Lawley Nathan , Azzopardi Anna , Gutkowski Allison , Niedziela Michelle Murphy , Horn Rachel , Kennedy David O. , Adamson Jason 
Published: January 2025

IntroductionFunctional beverages are increasingly popular but it is important to validate their purported effects through research. The aim of the current study was to investigate the effects of a new functional energy shot on cognitive performance and mood states in healthy adults, with a focus on measuring mental energy enhancement and attenuation of negative effects associated with extended performance of mentally demanding tasks.MethodsThis study was a randomized, double-blind, placebo-controlled, crossover trial. Thirty-seven healthy males and females, aged 18–30 years, consumed a functional energy shot (Ryde: Energize) or placebo on separate testing occasions one-week apart. Participants completed cognitive and mood assessments pre-dose, and then 30 minutes post-dose over the course of approximately 2 hours. The functional energy shot contained caffeine, ginseng, vitamins and taurine, while the placebo shot was matched for flavor but did not contain these additional ingredients.ResultsUse of the functional energy shot was associated with significant improvements over placebo in cognitive performance, as measured by the Cognitive Demand Battery, with enhanced global performance, task-specific accuracy and speed across repeated assessments. Additionally, the shot mitigated negative effects associated with extended cognitive tasks, reducing perceived mental fatigue and increasing perceived alertness and energy. Working memory tasks showed faster performance post-consumption, and mood assessments revealed positive effects on vigor, fatigue and overall mood disturbance.DiscussionThese results indicate wide cognitive and mood effects of this functional energy shot, potentially attributable to synergistic combination of active ingredients.Clinical trial registrationclinicaltrials.gov, identifier NCT06384586.
Long term outcomes associated with the use of perioperative systemic chemotherapy on low grade appendiceal mucinous neoplasms with pseudomyxoma peritonei treated with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy
Authors: Ruff Samantha M. , Kasumova Gyulnara , Palavalli Manoj , Eng Oliver S. , Lambert Laura , Clarke Callisia , Patel Sameer , Veerapong Jula , Fournier Keith , Abbott Daniel , Staley Charles , Grotz Travis , Johnston Fabian , Raoof Mustafa , Dineen Sean , Cloyd Jordan M. , Kim Alex C. 
Published: January 2025

IntroductionLow grade appendiceal mucinous neoplasms (LAMN) are indolent tumors that lack invasive potential but may present as pseudomyxoma peritonei. Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) significantly improves both overall and recurrence free survival. While systemic chemotherapy is generally considered ineffective for LAMN, little literature is available to support this notion. We evaluated outcomes for individuals with LAMN who did and did not receive systemic chemotherapy in combination with CRS+HIPEC.MethodsA multicenter retrospective cohort study was performed using the US HIPEC Collaborative that included patients with LAMN who underwent CRS+HIPEC. The overall survival (OS) and recurrence-free survival (RFS) of patients who did and did not receive systemic chemotherapy were compared. Survival and variables associated with survival were evaluated with the Kaplan-Meier analysis and cox regression, respectively.ResultsAmong the 529 included patients with LAMN, 63 (11.9%) received systemic chemotherapy and CRS+HIPEC, while 466 (88.1%) were treated with only CRS+HIPEC. Patients selected for systemic chemotherapy had a higher burden of disease (mean peritoneal cancer index: 18.8 +/- 8.6 versus 14.3 +/- 8.8, p&lt;0.001). Patients who were not treated with chemotherapy had better mean OS and RFS (OS: 104.3 +/- 6.2 months, RFS: 84.9 +/- 6.6 months) compared to those who underwent systemic chemotherapy (OS: 70.2 +/- 6.8 months, RFS: 38 +/- 5.9 months, p&lt;0.001). Increasing pre-operative CEA level (HR 1.012, p&lt;0.001), higher completeness of cytoreduction score (reference CCR 0, CCR2 HR 34.175, p=0.001 and CCR3 HR 52.041, p=0.001), and treatment with systemic chemotherapy (HR 4.196, p=0.045) were associated with worse OS.ConclusionsIn this multicenter retrospective study, the receipt of perioperative chemotherapy was associated with worse long-term outcomes among patients with LAMN undergoing CRS-HIPEC. Systemic chemotherapy may lead to patient deconditioning and contribute to worse long-term outcomes. It should not be recommended outside of a clinical trial.
Deciphering the oncogenic network: how C1QTNF1-AS1 modulates osteosarcoma through miR-34a-5p and glycolytic pathways
Authors: Zhang Yu , Lun Hailong , Zhu Naiqiang , Yang Ning , Ding Kaikai , Chen Bin , Chang Chengbing , Gu Haipeng , Liu Yanqi 
Published: January 2025

IntroductionOsteosarcoma (OS), a prevalent metastatic cancer among young individuals, is associated with a grim prognosis. Long non-coding RNAs (lncRNAs), including C1QTNF1-AS1, are pivotal regulators of cancer cell proliferation and motility. As an oncogene, C1QTNF1-AS1 is implicated in various tumor types, such as colorectal, pancreatic, hepatocellular carcinomas, and OS. The aim of this study was to investigate the functions and underlying mechanisms of C1QTNF1-AS1 in the progression of osteosarcoma.MethodsThis investigation focused on elucidating the functional roles and mechanisms of C1QTNF1-AS1 in OS cells. Bioinformatics tools were utilized to identify the interaction between microRNA miR-34a-5p and C1QTNF1-AS1, as well as the targeting of LDHA and PDK3 by miR-34a-5p. Dual-luciferase reporter assays and RNA immunoprecipitation were employed to validate these interactions. Expression profiles of C1QTNF1-AS1, miR-34a-5p, LDHA, and PDK3 in osteosarcoma cells were analyzed using RT-PCR and western blot analyses, revealing their intricate relationships. The impact of these molecules on OS cell proliferation, invasion, and migration was assessed through CCK-8, Transwell, and Cell scratch assay. Moreover, the effects on aerobic glycolysis in OS cells were examined by quantifying ATP levels, lactate production, glucose uptake capacity, and the extracellular acidification rate.ResultsThe findings indicated a significant decrease in C1QTNF1-AS1 expression levels in OS cells compared to normal osteoblasts. A parallel downregulation trend of miR-34a-5p was also observed in OS cells. Silencing C1QTNF1-AS1 led to a marked upregulation of LDHA and PDK3 in OS cells, which was partially attenuated by miR-34a-5p mimics. Functional evaluations demonstrated that suppression of C1QTNF1-AS1 accelerated OS cell growth, motility, invasiveness, and the Warburg effect. Conversely, the overexpression of miR-34a-5p mitigated these stimulatory effects, suggesting a regulatory role in modulating OS progression.DiscussionOur research emphasizes the critical role of C1QTNF1-AS1 in the pathogenesis of osteosarcoma (OS). We discovered that the downregulation of C1QTNF1-AS1 indirectly upregulates the expression of LDHA and PDK3 by suppressing miR-34a-5p, which functions as a regulator of the Warburg effect. This cascade of events promotes OS progression by enhancing glycolytic metabolism and supplying energy for cancer cell growth, migration, and invasion. These findings suggest a potential therapeutic target and highlight the importance of understanding the regulatory network involving lncRNAs in cancer metabolism and progression.
DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway
Authors: Zhao Rui , Wang Mengran , Wu Zeyu , Zhao Panpan , Dong Huiling , Su Yue , Zhao Chenghui , Qi Min , Ling Shizhang , Jiang Xiaochun 
Published: January 2025

IntroductionGliomas, particularly glioblastomas (GBM), are highly aggressive with a poor prognosis and low survival rate. Currently, deoxyelephantopin (DET) has shown promising anti-inflammatory and anti-tumor effects. Using clinical prognostic analysis, molecular docking, and network pharmacology, this study aims to explore the primary targets and signaling pathways to identify novel GBM treatment approaches.MethodsUsing PharmMapper, the chemical structure of DET was examined for possible targets after being acquired from PubChem. GBM-related targets were obtained through multi-omics approaches. A protein-protein interaction (PPI) network was constructed using Cytoscape and STRING, and target binding was evaluated through molecular docking. Enrichment analysis was conducted using Metascape. The effects of DET on GBM cell invasion, apoptosis, and proliferation were assessed through in vitro assays, including Transwell, EDU, CCK8, and flow cytometry. Western blot analysis was performed to examine the components of the PI3K/AKT signaling pathway.ResultsAmong the sixty-four shared targets identified, JUN and CCND1 were the most frequently observed. Enrichment analysis demonstrated that DET influenced the MAPK and PI3K/AKT signaling pathways. In Transwell assays, DET significantly inhibited the invasive behavior of glioma cells. Western blot analysis further confirmed the downregulation of EGFR, JUN, and PI3K/AKT.ConclusionDET inhibits GBM cell invasion, proliferation, and apoptosis via modulating the PI3K/AKT signaling pathway, highlighting its potential as a novel therapeutic strategy for GBM treatment.
Population pharmacokinetics and dosing optimization of imipenem in Chinese elderly patients
Authors: Wang Jing , Fang Qiu , Luo Xuemei , Jin Lu , Zhu Huaijun 
Published: January 2025

ObjectivesTo assess the pharmacokinetics and pharmacodynamics of imipenem in a retrospective cohort of hospitalized Chinese older patients.MethodsA population pharmacokinetic (PPK) model was constructed utilizing a nonlinear mixed-effects modeling approach. The final model underwent evaluation through bootstrap resampling and visual predictive checks. Additionally, a population pharmacokinetic and pharmacodynamic analysis was conducted employing Monte Carlo simulations to investigate the impact of commonly used dosing regimens (0.25 g every 6 h, 0.5 g every 6 h, 0.5 g every 8 h, 1 g every 6 h, 1 g every 8 h, and 1 g every 12 h) on the likelihood of achieving the target therapeutic outcomes.ResultsA total of 370 observations available from 142 patients were incorporated in the PPK model. A two-compartment PPK model with linear elimination best predicted the imipenem plasma concentrations, with the creatinine clearance as a significant covariate of clearance. Typical estimates for clearance, inter-compartmental clearance, central and peripheral volume were 13.1 L·h−1, 11.9 L·h−1, 11.7 L, 29.3 L, respectively.ConclusionThe pharmacokinetics of imipenem in elderly patients were effectively characterized by the established PPK model, which includes creatinine clearance as a key covariate. This research will enhance our understanding of imipenem elimination and support precision dosing in this patient demographic.
Conceptual framework of coaches’ decision-making in conventional sports
Authors: Kolar Edvard , Biloslavo Roberto , Pišot Rado , Veličković Saša , Tušak Matej 
Published: January 2025

IntroductionA coach’s managerial and pedagogical tasks in the sports training process constitute the substantive core of their work, while decision-making serves as the fundamental method underpinning these tasks. Some decisions made by coaches result from deliberate, analytical thinking, which involves extensive information gathering, analysis, and discussion. Others, however, are made quickly and spontaneously, triggered by unforeseen situations during training or competition that demand immediate action. Consequently, the purpose of this study is to develop a conceptual framework for understanding coaches’ decision-making behavior in conventional sports. This framework aims to establish appropriate relationships between the various decisions coaches make during the training process and theoretical concepts related to decision-making, both in general and within the coaching context.MethodsTo design the research, we used the methodology of a conceptual paper and a “model paper” approach, which seeks to build a theoretical framework that predicts relationships between distinct research concepts and scientific disciplines, aiming to integrate them into a cohesive model of coaches’ decision-making behavior.ResultsThe proposed conceptual framework encompasses a comprehensive range of situations that may arise during the sports training process and potential ways to address them. This framework identifies different types of decisions and characteristics associated with coaches’ decision-making behavior. It incorporates various sport-specific and general theories of decision-making and cognitive functioning to offer a deeper understanding of how coaches process and execute decisions in diverse contexts.DiscussionThe developed conceptual framework outlines three primary types of decisions—strategic, tactical, and operational—each playing a distinct role in the broader sports training process. These decisions are based on different cognitive processes, which manifest in varied decision-making behaviors and are reinforced by specific leadership styles. The practical value of this framework lies in its potential application for selecting appropriate experts to address the diverse decision-making scenarios encountered in sports training. This ensures the alignment of decision-making styles with the requirements of specific training situations, thereby enhancing the effectiveness and outcomes of the coaching process.
Effectiveness of a home-based computerized cognitive training in Parkinson's disease: a pilot randomized cross-over study
Authors: Tagliente Serena , Minafra Brigida , Aresta Simona , Santacesaria Paola , Buccoliero Andrea , Palmirotta Cinzia , Lagravinese Gianvito , Mongelli Davide , Gelao Christian , Macchitella Luigi , Pazzi Stefania , Scrutinio Domenico , Baiardi Paola , Battista Petronilla 
Published: January 2025

IntroductionCognitive symptoms are common in Parkinson's Disease (PD), and digital interventions like telerehabilitation other an accessible way to manage these symptoms. This study aimed to assess the effectiveness of a Home-Based Computerized Cognitive Training (HB-CCT) program in individuals with PD using a pilot randomized cross-over design.MethodsTwenty-five participants (mean age 69.32 ± 7.21 years, mean MDS-UPDRS III 33.76 ± 14.25) with PD and mild cognitive impairment were enrolled. They underwent neuropsychological assessments at three time points (5-week intervals): Baseline, after the HB-CCTi, and after Standard Care. The HB-CCT consisted of the Neurotablet® platform that was used to target cognitive domains such as Attention, Memory, Perception, Executive Functioning and Language. All participants completed both the Neurotablet intervention and Standard Care blocks in a randomized order. After a Shapiro-Wilk test, non-parametric repeated measures analyses of variance (Friedman's test) and post-hoc comparisons corrected with the Benjamini-Hochberg approach were performed to compare the effects on primary and secondary cognitive outcomes over experimental intervention and Standard Care.ResultsThe results from the Friedman analysis revealed significant improvements in Word List Immediate Recall, Digit Span Forward and Complex Figure Recall (all p &lt; 0.001) following the HB-CCT, compared to the Baseline. Additionally, Naming performance showed significant improvement after the HB-CCT (p = 0.02). Significant differences were also observed when comparing the HB-CCT with Standard Care, with improved performance in TMT-A (p = 0.02), Phonemic Fluency (p &lt; 0.01), and Digit Span Forward (p &lt; 0.01).DiscussionThese findings suggest that HB-CCT via Neurotablet can effectively enhance specific cognitive abilities in PD, supporting the role of digital, home-based interventions as feasible strategies to mitigate cognitive decline.
A comparative study of hospitalization costs of TKA inpatients before and after National Volume-based Procurement in Guangdong, China: an interrupted time-series analysis
Authors: Li Anqi , Li Gongduan , Han Dong , Fu Manru , Chang Jinghui 
Published: January 2025

BackgroundThis study aims to explore the impact of the National Volume-based Procurement Policy in Guangdong Province on hospitalization costs for total knee arthroplasty inpatients.MethodsInterrupted time-series analysis were used to examine the expenses associated with total knee arthroplasty for inpatients at a hospital in Guangzhou between May 10, 2021, and December 26, 2023. The period was divided into two phases based on the implementation of the policy, the pre-policy phase (May 10, 2021, to April 30, 2022) and the post-policy phase (May 1, 2022, to December 26, 2023). A total of ten indicators, derived from patients’ medical records, were analyzed. Indicators included total expenses, self-financed expenses, consumables costs, miscellaneous service fees, diagnostic fees, treatment fees, rehabilitation fees, total Chinese medicine costs, western medicine costs, and the costs of blood and blood products.ResultsA total of 1,196 valid cases were included, with 290 cases before policy implementation and 906 cases after implementation. When the implementation of procurement occurred (May 1, 2022), total expenses (β2= − 28240.17, p &lt; 0.001), consumables costs (β2= − 31302.72, p &lt; 0.001), and self-financed expenses (β2= − 13674.56, p&lt;0.001) showed an instantaneous decreasing trend. The mean total expenses decreased from 65,324.73 CNY per case to 34,465.57 CNY per case, representing a reduction of 30,859.16 CNY per case. Miscellaneous service fees (β2= 440.45, p &lt; 0.05), diagnostic fees (β2=746.00, p &lt; 0.05), rehabilitation fees (β2=207.36, p &lt; 0.001) exhibited an instantaneous and slight increasing trend. After implementation (from May 1, 2022 to December 26, 2023), the total expenses (β3=−106.95, p&lt;0.05), consumables costs (β3= − 65.05, p&lt;0.05), diagnostic fees (β3= − 22.44, p &lt; 0.05), treatment fees (β3= − 28.01, p &lt; 0.05), total Chinese medicine costs (β3= − 9.98, p &lt; 0.05), and blood and blood products costs (β3= − 5.88, p&lt;0.05) continued to decrease.ConclusionThe volume-based procurement policy in Guangdong, China has shown initial effectiveness. After it was implemented, total expenses, consumables costs, and self-financed expenses decreased. The structure of hospitalization costs also reflects the slight improvement in healthcare providers’ value and treatment planning decisions. Further attention should be given to the healthcare professionals’ labor value. The empirical analysis results provide a reference for the government further to improve the volume-based procurement for artificial joints.