bluuebunny/crossref_metadata_2025_split huggingface dataset:
First cytogenetic characterization of the parasitoid wasp Brachymeria (Pseudobrachymeria) vesparum Bouček, 1992 (Hymenoptera, Chalcididae)
Authors: Santos Rafael de Jesus , Pinheiro Tailan Silva , Bitencourt Jamille de Araújo , Miranda Adrianne Oliveira , Júnior Juvenal Cordeiro Silva 
Published: January 2023

Abstract
Cytogenetic analyses have been widely used to understand evolutionary processes and to resolve taxonomic uncertainties in insects. In particular, the mapping of specific chromosomal regions might provide insights about the genome organization and interspecific relationships. Considering the importance of this approach and the scarcity of chromosomal data in some groups of Hymenoptera, this study provides the first cytogenetic characterization of the parasitoid wasp Brachymeria (Pseudobrachymeria) vesparum Bouček,1992. This species was characterized by 2n = 10 metacentric chromosomes. The heterochromatin was located at pericentromeric and terminal regions, being particularly conspicuous, occupying a large part of chromosomes from pair 2. In addition, GC+ sites were detected at interstitial region of two chromosomal pairs. The present data was useful to infer the chromosomal rearrangements involved in speciation within Brachymeria besides providing cytotaxonomic markers for further comparative cytogenetic studies.
Investigating lake drought prevention using a DRL-based method
Authors: ghayoomi hadi , Partohaghighi Mohammad 
Published: January 2023

Abstract
Drought and decrease in the level of lakes in recent years due to global warming and excessive use of water resources feeding lakes is of great importance and this research has provided a structure to investigate this issue. First, the information required for simulating lake drought is provided with strong references and necessary assumptions. Entity-Component-System (ECS) structure has been used for simulation which can consider assumptions flexibly in simulation. Three major users (i.e., Industry, agriculture, and Domestic users) consume water from ground water and surface water (i.e., streams, rivers and lakes). Lake Mead has been considered for simulation, and the information necessary to investigate its drought has also been provided. The results are presented in the form of scenario-based design and optimal strategy selection. For optimal strategy selection a deep reinforcement algorithm is developed to select the best set of strategies among all possible projects. These results can provide a better view of how to plan to prevent lake drought.
WITHDRAWN: Application of multiple occupational health risk assessment models for metal fumes in welding process
Authors: Pourhassan Bahman , Nasirzadeh Nafiseh , Beigzadeh Zahra , Karimi Ali 
Published: January 2023

Abstract
Welding fumes have an important role to create the adverse health effects. So, the aim of this study was to use of multiple occupational health risk assessment models for metal fumes in welding process.
This cross-sectional study was conducted among welding workers. Sampling of heavy metals such as Sn, Zn, Al, Fe, Cd, Pb, Cu, Mn, Ni, Cr, and As was provided based on the National Institute for Occupational Safety and Health (NIOSH) method 7300 and analyzed by inductively coupled plasma mass spectroscopy (ICP-MS). Risk assessment was managed by four methods including Malaysia's method, Control of Substances Hazardous to Health Essentials (COSHH model), Chinese OHRA standard (GBZ/T 298-2017) and EPA method. Also, Monte Carlo simulation was used to examine the uncertainties in EPA method by using the Crystal Ball tool. To compare the models, the risk levels of each model were converted into the risk ratio and SPSS 22.0 software was used to the statistical analysis. The consistency of the two occupational health risk assessment models was examined by Cohen's Kappa.
Risk ration was the highest level for Cr (VI) fumes in all models. Also, carcinogenic risk was unacceptable for all examined fumes. Moreover, non-carcinogenic risk was the highest (HI>1) for As fumes. Mont Carlo simulationssuggested that exposure time (ET) had a significant effect on the risk. Also, there was a good consistency between Malaysia method/ GBZ/T 298-2017 and COSHH model/GBZ/T 298-2017.
Therefore, it is recommended that the engineering and administrative controls should be provided to reduce exposure.
Exposed Population to Hot and Cold Extremes in MENA for Paris Climate Agreement Temperature Goals
Authors: Hamed Mohammed Magdy , Ali Zulfiqar , Nashwan Mohamed Salem , Shahid Shamsuddin 
Published: January 2023

Abstract
This study aims to project extreme temperatures and the population exposed to them in the MENA region for two Shared Socioeconomic Pathways (SSP1-1.9 and 1-2.6), representative Paris climate agreement goals of 1.5 and 2.0°C temperature rise limits, respectively, for two future periods, near (2020-2059) and far (2060-2099). The daily maximum (Tmax) and minimum (Tmin) temperature of Global Climate Models (GCMs) of the Coupled Model inter-comparison Project Phase 6 (CMIP6) were used to estimate twelve temperature indices, while the population distribution for the historical and future periods was used to estimate the changes in the population exposed to temperature extremes. The results revealed an increase in days with Tmax>45°C (D45Tmax) by 30 and the population exposure to D45Tmax by 20 million person-day by 2099 for SSP1-2.6. In contrast, there will be a decrease in the days with Tmin<0°C (D0Tmin) by 11, which would cause a decrease in the population exposed to it by 36 and 480 million person-days in the far future for both scenarios. The change in temperature extremes would mostly affect the populations in Mauritania, Algeria, Morocco, Saudi Arabia, Iraq, UAE and Qatar. For a temperature rise of 2.0 °C, the percentage of the population exposed to the hot extreme will increase between 0.5 and 5.2% by 2059 and between 1.8 and 18.1% by 2099, indicating a significant increase in the population exposed to the hot extreme for only 0.5 °C rising temperature. However, the overall impact will be more in reducing the cold extremes than increasing the hot extreme.
Australian fire weather variability using calibrated long-term reanalysis data
Authors: Biswas Soubhik , Chand Savin S. , Dowdy Andrew J. , Wright Wendy , Foale Cameron , Zhao Xiaohui 
Published: January 2023

Abstract
Spatiotemporal variability on multi-decadal time scales can be observed in weather conditions associated with wildfires. The climatic factors that drive this variability in fire weather conditions have been studied using bias-corrected 20th Century Reanalysis data of the mean state and extreme values of the McArthur Forest Fire Danger Index (FFDI) from 1876 to 2014. In particular, the association of this time series with the climate drivers of El Niño-Southern Oscillation (ENSO), Indian Ocean Dipole (IOD), Southern Annular Mode (SAM) and Interdecadal Pacific Oscillation (IPO) are analysed using several techniques. The potential for long-term changes in the relationships between the climate drivers and seasonal FFDI values is also explored by breaking the total time period into two epochs. Composite analysis was performed to separate the phases of ENSO, IOD and SAM and determine which phase combinations influence severe fire weather conditions the most. The most severe fire weather conditions were observed during a specific combination of ENSO, IOD and SAM phases, with results mapped to show spatial and seasonal variations in this over Australia. Long-term changes in the relationships between fire weather conditions and the three climate drivers (ESNO, IOD, SAM and IPO) are documented. These findings are based on a longer time period than similar previous studies, intended to help contribute to the range of guidance available for long-range fire weather outlooks and enhanced decision-making for fire agencies.
Risk factors for postoperative fever after laparoscopic adrenalectomy focusing on hormones produced.
Authors: Izawa Mizuki , Takeda Toshikazu , Anno Tadanori , Yasumizu Yota , Tanaka Nobuyuki , Matsumoto Kazuhiro , Morita Shinya , Kosaka Takeo , Mizuno Ryuichi , Asanuma Hiroshi , Oya Mototsugu 
Published: January 2023

Abstract
Purpose: Laparoscopic adrenalectomy is widely performed for a number of hormone-producing tumors and postoperative management depends on the hormones produced. In the present study, we conducted a retrospective analysis to clarify the risk factors for postoperative complications, particularly postoperative fever after laparoscopic adrenalectomy.
Methods: We analyzed 406 patients who underwent laparoscopic adrenalectomy at our hospital between 2003 and 2019. Postoperative fever was defined as a fever of 38°C or higher within 72 hours after surgery. We investigated the risk factors for postoperative fever after laparoscopic adrenalectomy.
Results: There were 188 males (46%) and 218 females (54%) with a median age of 52 years. Among these patients, tumor pathologies included 188 primary aldosteronism (46%), 75 Cushing syndrome (18%), and 80 pheochromocytoma (20%). Postoperative fever developed in 124 of all patients (31%), 30% of those with primary aldosteronism, 53% of those with pheochromocytoma, and 8% of those with Cushing syndrome. A multivariate logistic regression analysis identified pheochromocytoma and non-Cushing syndrome as independent predictors of postoperative fever. Postoperative fever was observed in 42 out of 80 cases of pheochromocytoma (53%), which was significantly higher than in cases of non-pheochromocytoma (82/326, 25%, p<0.01). In contrast, postoperative fever developed in 6 out of 75 cases of Cushing syndrome (8%), which was significantly lower than in cases of non-Cushing syndrome (118/331, 35.6%, p<0.01).
Conclusion: Since postoperative fever after laparoscopic adrenalectomy is markedly affected by the hormone produced by pheochromocytoma and Cushing syndrome, it is important to carefully consider the need for treatment.
How do farmers' perceptions and attitudes toward agricultural water consumption behaviors can lead to unsustainability; evidence from Mahabad plain, Lake Urmia, Iran
Authors: Farahmand Hamid , Tajrishy Massoud , Isaai Mohammad Taghi , Ghoreishi Mohammad , Mohammadi Mohammadreza 
Published: January 2023

Abstract
There has been much attention paid to Lake Urmia's catastrophic desiccation by researchers and the government. An in-depth semi-structured interview and thematic analysis were used in this study to examine irrigation behavior and crop type selection decisions. 73% of farmers believe that there is no need to reduce their water consumption, 87% do not look for rain forecasts since they regard the government as responsible for water supply or have very few crop alternatives to choose from. In choosing the type of product, 77% only consider economics and do not consider environmental objectives, and 71% do not think drought conditions affect irrigation decisions. Educating farmers and increasing their collaboration role are therefore necessary. Therefore, these variables are the basis for extending psychological theories such as TPB to predict farmers' behavior to a much greater extent. While this study focused on one region, its findings are applicable to similar circumstances worldwide.
Automated and Rapid Chemiluminescence Immunoassay for Cardiac Troponin I Based on Digital Microfluidics
Authors: Cheng Kangning , Ding Yue , Liu Cong , Ding Yanjing , Xie Shuqi , Zhu Xiaofeng , Liu Huanqing , Yue Weiwei 
Published: January 2023

Abstract
Rapid diagnosis of acute myocardial infarction (AMI) is critical in the clinical setting. An automated and rapid chemiluminescence immunoassay system based on digital microfluidics (DMF) is suggested for cardiac troponin I (cTnI). This method involves a double antibody sandwich structure. The cTnI antigen in the sample to be analyzed was captured by magnetic beads encapsulated with the cTnI antibody, which was subsequently recognized by the biotin-labeled secondary antibody. Horseradish peroxidase (HRP)-labeled streptavidin may be conjugated to biotin and used to mark the cTnI antigen because of the unique way that biotin binds to avidin. The simultaneous recognition of the cTnI antigen by primary and secondary antibodies in sandwich form, as well as the high specificity of the reaction between streptavidin and biotin, considerably improves the sensitivity and specificity of this technique for cTnI detection. This approach has decent stability. For cTnI samples, the full process took 40 minutes, with a limit of detection (LOD) of 0.39 ng mL− 1. Additionally, there was a significant correlation range from 0.39 to 25 ng mL− 1, with a correlation value of 0.994. The CV (Coefficient of variation) value was 2.26%. This system is anticipated to be used for the clinical measurement of cTnI in human serum.
The prognostic significance of co-expression of α-SMA and Trichrome in the tumor microenvironment of poorly differentiated endometrial carcinoma
Authors: Chen Xi , Cheng Yuan , Zhao Na , Liu Fangfang , Wang Jianliu 
Published: January 2023

Abstract
Backgroud:
In recent years, the relationship between tumor microenvironment (TME) and adjuvant therapy has become a research hotspot. α-SMA is heterogeneous in different tumors. Type I collagen deposition influenced the access of immune cells to cancer cells. This study aims to describe the prognostic significance of co-expression of α-SMA and Trichrome in the tumor microenvironment of poorly differentiated endometrial carcinoma.
Methods
A total of 45 cases of poorly differentiated endometrial carcinoma (EC) from March 2013 to December 2020 was collected in the study. There were 17 cases of grade 3 endometrioid endometrial carcinomas (EECs), 16 cases of endometrial serous carcinomas, and 12 cases of endometrial clear cell carcinomas. The expression of α-SMA, CD10, and desmin was detected in the tumor microenvironment (TME) by the immunohistochemical method. Trichrome was detected to identify collagen in TME by a special dyeing method. Between-group comparison in categorical variables was assessed via using Fisher’s exact test. The overall survival was analyzed by a Kaplan-Meier curve.
Results
The total expression rate of α-SMA in endometrial carcinoma was 71.1%. The expression rate of α-SMA was 82.4%, 62.5%, and 66.7% positively expressed in EEC, serous carcinoma, and clear cell carcinoma, respectively. CD10 and desmin were negative in all EC TME. Both α-SMA and Trichrome positive groups showed significantly higher mortality rates (p = 0.042) and poorer prognosis (p = 0.041) than both α-SMA and Trichrome negative groups.
Conclusions
The co-expression of α-SMA and Trichrome is of great significance in predicting the poor prognosis of endometrial cancer.
Phylogenetic and geographic diversification/differentiation as an evolutionary avenue in the genus Cephalocereus (Cactaceae) Evolutionary Avenue in Cephalocereus
Authors: Tapia Héctor J. , Arias Salvador , Morrone Juan J. , Dávila Patricia 
Published: January 2023

Abstract
Phylogenetic and geographic distances were estimated to produce a combined Distancing Index as a measure of historical reproductive isolation in the genus Cephalocereus. Geographic and climatic barriers were inferred from potential and observed distributions. Distances were extracted from a Bayesian tree for seven chloroplast regions and 26 terminals, and from the geolocation dataset for the exact same sampling. Distance matrices were relativized, and a Mantel test was applied to identify deviations between phylogenetic and geographic distances. Heatmaps and scatterplots were implemented to visualize combined trends. Two basal clades show patterns of differentiation or complete isolation; the first includes C. scoparius, C. apicicephalium, C. nizandensis, and C. totolapensis, and the second includes C. parvispinus, C. polylophus, and C. euphorbioides. The species C. fulviceps, and C. sanchezmejoradae appear in a differentiated grade as sisters of a well-defined clade that includes C. mezcalaensis, C. macrocephalus, C. tetetzo, C. senilis, C. columna-trajani, C. multiareolatus, and C. nudus, where geographic or phylogenetic distances lie below the mean, indicating a diversification process in absence of hard barriers. At the generic level, separation is related to climatic factors as temperature and moisture, while factors as the altitude could be determinants of separation at the species level. The steady accumulation of variants may lead to opposed evolutionary outcomes: differentiation or diversification, in isolated and non-isolated lineages, respectively. More studies are needed on how genetic variation is transferred or interchanged between and among lineages, and how morphological differentiation of diverging lineages account for reproductive isolation.
Social Media Insights for Neurosurgical Oncologists: A Survey of the American Association of Neurological Surgeons and Congress of Neurological Surgeons Joint Section on Tumors
Authors: Haider Ali S. , Zaki Peter G. , Mehta Gautam U. , Sheehan Jason P. , Shepard Matthew J. 
Published: January 2023

Abstract
Purpose
There is a paucity in the literature regarding the characteristics and attitudes of social media (SM) utilization in a professional manner by neurosurgical oncologists.
Methods
A 34-question electronic survey was created using Google Forms and disseminated via email to members of the AANS/CNS Joint Section on Tumors. Demographic data were compared amongst those who utilize social media versus those who do not. Factors associated with positive outcomes of professional SM use and with having more followers on SM were analyzed.
Results
The survey received 94 responses, of which 64.9% reported that they currently use SM in a professional manner. Age < 50 years was found to be associated with SM use (p = 0.038). Facebook (54.1%), Twitter (60.7%), Instagram (41%), and LinkedIn (60.7%) were the most used SM platforms. Having a higher number of followers was associated with practicing in academics (p = 0.005), using Twitter (p = 0.013), posting about their own research publications (p = 0.018), posting interesting cases (p = 0.022), and posting about upcoming events (p = 0.001). Having a higher number of followers on SM was also associated with positive outcomes, specifically new patient referrals (p = 0.04).
Conclusions
Neurosurgical oncologists can benefit by using social media professionally for increased patient engagement and networking within the medical community. Practicing in academics, making use of Twitter, and posting about interesting cases, upcoming academic events, and one’s own research publications can help gain followers. In addition, having a large following on social media could lead to positive outcomes such as new patient referrals.
A Novel Hybrid Algorithm for Acceleration Harmonic Estimation and Elimination in Shake Table
Authors: Malathy R. B. , Bhat Govardhan Bhat , Dewangan U.K. 
Published: January 2023

Abstract
Shake table tests instantly submits structure models for seismic excitation and evaluates its overall performance. However, inconsistencies in the amplified seismic output will undoubtedly exist and the desired signals cannot be precisely achieved. The ability to accurately estimate and analyze system harmonics is necessary for building control filters that hinder harmonic levels in the signals. This paper initially focus on studying the key techniques such as time integration algorithms, filtering techniques and metaheuristic optimization methods to understand its applications, and their implementation in shake tables. Then, a novel hybrid algorithm that has the capability of regulating the weights of the unknown parameter of harmonic signal arising in the table is formulated. A simulation and experimental study is carried out and the developed algorithm is also compared to Artificial Bee Colony (ABC) algorithm, Particle Swarm Optimization (PSO) algorithm and Modified Ant Bee Colony-Recursive least squares (MABC-RLS) algorithm, and studied in terms of performance. Finally, future research and application challenges are discussed and underlined.
A novel Arabidopsis peptide represses phloem differentiation via autocrine and paracrine signaling
Authors: Hazak Ora , Carbonnel Samy , Cornelis Salves 
Published: January 2023

Abstract
Plant meristems require a constant supply of photoassimilates and hormones to the dividing meristematic cells. In the growing root, such supply is delivered by protophloem sieve elements (1). Due to its preeminent function for the root apical meristem, protophloem is the first tissue to differentiate. This process is regulated by a genetic circuit involving in one-side the positive regulators DOF transcription factors (2, 3), OCTOPUS (OPS)(4) and BREVIX RADIX (BRX)(5), and in the other-side the negative regulators CLAVATA3/EMBRYO SURROUNDING REGION RELATED (CLE) peptides and their cognate receptors BARELY ANY MERISTEM (BAM) receptor-like kinases and co-receptors CLAVATA3 INSENSITIVE RECEPTOR KINASES (CIKs)(2, 6-8). brx and ops mutants harbor a discontinuous protophloem (4, 5) that can be fully rescued by mutation in BAM3 (5) but only partially rescued by multiple mutations in all three known phloem-specific CLE genes, CLE25/26/45 (2, 6). These observations suggest that one or more additional CLE peptides play a role in protophloem formation. By reanalyzing Arabidopsis genome, we have identified a novel CLE gene closely related to CLE45, named CLE33, that is expressed in developing protophloem and is perceived by BAM3. Mutations in CLE33 and CLE45 are together sufficient to fully suppress brx and ops discontinuous protophloem phenotype. Furthermore, in these mutants we observed ectopic protophloem differentiation in neighboring cells, supporting the current model where phloem-specific CLE peptides act as paracrine signals to maintain a single functional protophloem cell file (2). CLE33 orthologs are found in basal angiosperms, monocots and eudicots, and the gene duplication giving rise to CLE45 in Arabidopsis and other Brassicaceae appears to be a recent event. We, thus, have discovered a novel peptide gene that is an ancient angiosperms’ CLE gene, and an essential player in the genetic circuit controlling protophloem formation.