bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between altered intestinal microbiome, impaired systemic and ocular surface immunity, and impaired wound healing response after corneal alkaline-chemical injury in diabetic mice
Authors: Bu Yashan , Shih Kendrick Co , Wong Ho Lam , Kwok Sum Sum , Lo Amy Cheuk-Yin , Chan Joseph Yau-Kei , Ng Alex Lap-Ki , Chan Tommy Chung-Yan , Jhanji Vishal , Tong Louis 
Published: January 2023

PurposeWe aim to investigate the effect of sustained hyperglycemia on corneal epithelial wound healing, ocular surface and systemic immune response, and microbiome indices in diabetic mice compared to controls after alkaline chemical injury of the eye.MethodsCorneal alkaline injury was induced in the right eye of Ins2Akita (Akita) mice and wild-type mice. The groups were observed at baseline and subsequently days 0, 3, and 7 after injury. Corneal re-epithelialization was observed under slit lamp with fluorescein staining using a cobalt blue light filter. Enucleated cornea specimens were compared at baseline and after injury for changes in cornea thickness under hematoxylin and eosin staining. Tear cytokine and growth factor levels were measured using protein microarray assay and compared between groups and time points. Flow cytometry was conducted on peripheral blood and ocular surface samples to determine CD3+CD4+ cell count. Fecal samples were collected, and gut microbiota composition and diversity pattern were measured using shotgun sequencing.ResultsAkita mice had significantly delayed corneal wound healing compared to controls. This was associated with a reduction in tear levels of vascular endothelial growth factor A, angiopoietin 2, and insulin growth factor 1 on days 0, 3, and 7 after injury. Furthermore, there was a distinct lack of upregulation of peripheral blood and ocular surface CD3+CD4+ cell counts in response to injury in Akita mice compared to controls. This was associated with a reduction in intestinal microbiome diversity indices in Akita mice compared to controls after injury. Specifically, there was a lower abundance of Firmicutes bacterium M10-2 in Akita mice compared to controls after injury.ConclusionIn diabetic mice, impaired cornea wound healing was associated with an inability to mount systemic and local immune response to ocular chemical injury. Baseline and post-injury differences in intestinal microbial diversity and abundance patterns between diabetic mice and controls may potentially play a role in this altered response.
Peptidomics analysis of plasma in patients with ankylosing spondylitis
Authors: Zhang Guo-ning , Xu Ying-jia , Jin Lei 
Published: January 2023

BackgroundThis study aimed to explore the differential expression of peptides associated with ankylosing spondylitis (AS) patients, enabling identification of potential functional peptides to provide the basis for the novel intervention targets for AS.Material and Methods3 AS patients and 3 healthy volunteers were enrolled in this study. The expression profiles for peptides present in the plasma of AS patients and the healthy individual were analyzed by liquid chromatography-tandem mass spectrometry (LC‐MS/MS). The physicochemical properties and biological functions of identified peptides were further analyzed by bioinformatics. The results of peptide identification were verified by cell viability analysis, using CCK8 and Edu staining assay, and the differential peptides relevant to the disease were screened.Results52 differential peptides were successfully identified using mass spectrometry. 44 peptides were up-regulated, while eight were down-regulated. FGA-peptide (sequences: DSGEGDFLAEGGGVRGPR), C4A-peptide (sequences: NGFKSHAL), and TUBB-peptide (sequences: ISEQFTAMFR) were screened out that could significantly promote the proliferation of fibroblasts in AS patients. Bioinformatics analysis showed these differentially expressed peptides might be associated with “MHC class I protein binding” and “pathogenic Escherichia coli infection” pathways, which might further affect the progression of AS.ConclusionThis pilot study shows 3 differentially expressed peptides may have the potential function for the occurrence and development of AS, may provide novel insights into the underlying molecular mechanisms of AS based on peptide omics.
Characterization of METRNβ as a novel biomarker of Coronavirus disease 2019 severity and prognosis
Authors: Gao Xun , Chan Paul Kay-Sheung , Wong Katie Ching-Yau , Ng Rita Wai-Yin , Yeung Apple Chung-Man , Lui Grace Chung-Yan , Ling Lowell , Hui David Shu-Cheong , Huang Danqi , Wong Chun-Kwok 
Published: January 2023

IntroductionCoronavirus disease 2019 (COVID-19) is increasing worldwide, with complications due to frequent viral mutations, an intricate pathophysiology, and variable host immune responses. Biomarkers with predictive and prognostic value are crucial but lacking.MethodsSerum samples from authentic and D614G variant (non-Omicron), and Omicron-SARS-CoV-2 infected patients were collected for METRNβ detection and longitudinal cytokine/chemokine analysis. Correlation analyses were performed to compare the relationships between serum METRNβ levels and cytokines/chemokines, laboratory parameters, and disease severity. Receiver operating characteristic (ROC) curves and Kaplan-Meier survival curves were used to evaluate the predictive value of METRNβ in COVID-19.ResultsThe serum level of METRNβ was highly elevated in non-Omicron-SARS-CoV-2 infected patients compared to healthy individuals, and the non-survivor displayed higher METRNβ levels than survivors among the critical ones. METRNβ concentration showed positive correlation with viral load in NAPS. ROC curve showed that a baseline METRNβ level of 1886.89 pg/ml distinguished COVID-19 patients from non-infected individuals with an AUC of 0.830. Longitudinal analysis of cytokine/chemokine profiles revealed a positive correlation between METRNβ and pro-inflammatory cytokines such as IL6, and an inverse correlation with soluble CD40L (sCD40L). Higher METRNβ was associated with increased mortality. These findings were validated in a second and third cohort of COVID-19 patients identified in a subsequent wave.DiscussionOur study uncovered the precise role of METRNβ in predicting the severity of COVID-19, thus providing a scientific basis for further evaluation of the role of METRNβ in triage therapeutic strategies.
Discussing sexuality with patients with neurological diseases: A survey among neurologists working in Saudi Arabia
Authors: Almalki Daifallah Mohammed , Kotb Mamdouh Ali , Albarrak Anas Mohammed 
Published: January 2023

BackgroundNeurological diseases frequently affect sexual activity, and the resulting sexual dysfunction can cause much distress for patients. However, despite the importance of such complaints, neurologists frequently do not ask patients about their sexual symptoms or how their neurological illness and medications are affecting their sexual health. This study aimed to identify these difficulties as well as potential obstructions to conversations for addressing sexual dysfunction in patients with neurological diseases.MethodsThis cross-sectional study was performed by sending invitation letters and questionnaires to registered neurologists in Saudi Arabia. The questionnaire was constructed to determine the possibility of discussing sexual activities and function with patients with neurological diseases and the possible obstacles neurologists face in this regard. Statistical analyses were performed using the Statistical Package of Social Sciences (SPSS) program version 25, and p-values of &lt;0.05 were considered statistically significant.ResultsA total of 258 of 750 neurologists (34.4%) returned the survey, of which 252 had completed the entire survey; therefore, their responses were considered suitable for further analysis. The majority of the respondents (63.1%) seldom discussed sexuality with their patients, more than half of the participants never discussed sexuality with female patients, and patients aged 60 years or older. The most commonly reported barriers were the lack of spontaneous communication by patients regarding their sexual problems (82.1%), insufficient consultation time (60.7%), and barriers based on language/culture/religion (53.6%). The majority of the respondents (61.9%) expressed the need for training on discussing sexuality as a measure that may enhance the discussion of sexual life with patients. Most of the respondents (92.9%) considered the patients responsible for bringing up problems in their sexual functioning during a patient interview.ConclusionSexual dysfunction is rarely discussed with patients showing neurological diseases, particularly with female patients. This is due to the patient's inability to articulate their sexual problems freely as well as a lack of consultation time. Training on discussing sexuality may enhance the discussion of sexual life with patients.
A multicenter randomized trial for quality of life evaluation by non-invasive intelligent tools during post-curative treatment follow-up for head and neck cancer: Clinical study protocol
Authors: Cavalieri Stefano , Vener Claudia , LeBlanc Marissa , Lopez-Perez Laura , Fico Giuseppe , Resteghini Carlo , Monzani Dario , Marton Giulia , Pravettoni Gabriella , Moreira-Soares Mauricio , Filippidou Despina Elizabeth , Almeida Aitor , Bilbao Aritz , Mehanna Hisham , Singer Susanne , Thomas Steve , Lacerenza Luca , Manfuso Alfonso , Copelli Chiara , Mercalli Franco , Frigessi Arnoldo , Martinelli Elena , Licitra Lisa ,   BD4QoL Consortium
Published: January 2023

Patients surviving head and neck cancer (HNC) suffer from high physical, psychological, and socioeconomic burdens. Achieving cancer-free survival with an optimal quality of life (QoL) is the primary goal for HNC patient management. So, maintaining lifelong surveillance is critical. An ambitious goal would be to carry this out through the advanced analysis of environmental, emotional, and behavioral data unobtrusively collected from mobile devices. The aim of this clinical trial is to reduce, with non-invasive tools (i.e., patients’ mobile devices), the proportion of HNC survivors (i.e., having completed their curative treatment from 3 months to 10 years) experiencing a clinically relevant reduction in QoL during follow-up. The Big Data for Quality of Life (BD4QoL) study is an international, multicenter, randomized (2:1), open-label trial. The primary endpoint is a clinically relevant global health-related EORTC QLQ-C30 QoL deterioration (decrease ≥10 points) at any point during 24 months post-treatment follow-up. The target sample size is 420 patients. Patients will be randomized to be followed up using the BD4QoL platform or per standard clinical practice. The BD4QoL platform includes a set of services to allow patients monitoring and empowerment through two main tools: a mobile application installed on participants’ smartphones, that includes a chatbot for e-coaching, and the Point of Care dashboard, to let the investigators manage patients data. In both arms, participants will be asked to complete QoL questionnaires at study entry and once every 6 months, and will undergo post-treatment follow up as per clinical practice. Patients randomized to the intervention arm (n=280) will receive access to the BD4QoL platform, those in the control arm (n=140) will not. Eligibility criteria include completing curative treatments for non-metastatic HNC and the use of an Android-based smartphone. Patients undergoing active treatments or with synchronous cancers are excluded.Clinical Trial Registration: ClinicalTrials.gov, identifier (NCT05315570).
Research on the innovation of time-honored brands from the perspective of dual ethical patterns
Authors: Ke Di , Li Guodong , Jiang Yichen , Li Yuanyuan , Liu Yi 
Published: January 2023

IntroductionInnovation has become an important means to promote the high-quality development of time-honored brands. However, the research on how to stimulate innovation investment in time-honored brands, especially that conducted in the local context, is still rare. To supplement the research limitations, this study adopts the perspective of dual ethical patterns and is set in the domestic context to explore the ethical influence of traditional cultures and marketization on the innovation investment of time-honored brands.MethodsWe proposed two complementary methods, which are OLS regression and fsQCA analysis respectively, to systematically analyze the mechanism and key path of the dual ethical pattern to promote the innovation of time-honored brands.ResultsThe results show that traditional culture and marketization level are both positively related to the innovation of time-honored brands. However, traditional culture and marketization level are mutually exclusive in their process of affecting the innovation of time-honored brands.DiscussionThis paper advances time-honored brands literature by highlighting the dual ethic pattern formed by traditional culture and marketization level on the innovation investment of time-honored brands. The findings respond to the academic debate between traditional culture and the innovation of time-honored brands, while enriching the research scope on the innovation mechanism of time-honored brands in the local context.
Preliminary data on the development of emotion vocabulary in typically developing children (5–13 years) using an experimental psycholinguistic measure
Authors: Sturrock Alexandra , Freed Jenny 
Published: January 2023

IntroductionVocabulary of emotion is integral to emotional development and emotional intelligence is associated with improved mental health outcomes. Many language disordered groups experience emotional difficulties; Developmental Language Disorders, Attention Deficit Hyperactivity Disorder and autism. However, (as in the case of autism) research tends to focus on assessing recognition of emotional states, rather than exploring labeling skills. Where labeling is assessed, measures have focused on early-acquired vocabulary (happy, sad, angry) or self/parent reporting. To date, no objective assessment has been made of vocabulary of emotion across childhood.MethodsThis study uses an experimental psycholinguistic measure, The Emotion Vocabulary: Expressive and Receptive ability measure (EVER) which includes two tasks (receptive vocabulary and word generation/expressive vocabulary). This measure has capacity to demonstrate vocabulary growth across age groups. 171 participants (5.0–13.11 years) completed The EVER Measure, alongside two closely matched standardized measures of basic language: BPVS (receptive vocabulary task) and CELF (word-association task). Assessments were completed online and en vivo (COVID testing restrictions dependent).ResultsAs predicted, children’s accuracy increased on both receptive and expressive emotion vocabulary tasks, in line with age at time of testing. EVER scores were significantly predicted by age and correlated with matched basic language scores. Secondary analysis provided preliminary findings on age of acquisition for specific emotion vocabulary items.DiscussionThe findings consequently demonstrate proof of concept for the use of The EVER Measure in assessing emotional vocabulary across childhood. This study provides important preliminary data on generating and recognizing emotion labels across typical child development. Critically, it extends current knowledge on emotion vocabulary acquisition into middle childhood, where linguistic ability is relatively mature. As such, findings have implications for research with potential clinical application in the assessment of older children, with either language or emotional differences or both. Findings demonstrate the need for a standardized tool, and its potential application in research and clinical practice is explored. A large-scale study offering proof of concept and reliability of The EVER Measure is indicated.
Noticias falsas la otra cara de la Pandemia. Caso: Ecuador.
Authors: Viteri Lozano Alexis Estephany , Carvajal Muñoz María Fernanda 
Published: January 2023

En una sociedad como la nuestra, la comunidad de usuarios de internet se convirtió en un consumidor, generador y repartidor de contenido de diversa índole. La mezcla de una pandemia por Covid-19, un confinamiento obligatorio y el escaso manejo responsable de la información digital contribuyó en la intensificación de las “fake news”.
Asimismo, la inundación de contenido en las plataformas sociales trajo consigo una “infodemia” reflejada en diversos tipos de desinformación entre ellos: la exageración, la descontextualización y el engaño.
Las plataformas digitales más usadas para su difusión son Facebook y WhatsApp. En el presente trabajo se analizaron 248 noticias calificadas como falsas por la Secretaría General de Comunicación, lo que corresponde a un muestreo probabilístico.
Pattern of oral anticoagulant prescribing for atrial fibrillation in general practice: an observational study in The Netherlands
Authors: Kager Catharina CM , Horsselenberg Maaike , Korevaar Joke C , Wagner Cordula , Hek Karin 
Published: January 2023

BackgroundIn the Dutch atrial fibrillation (AF) guideline for GPs, vitamin K antagonists (VKAs) and direct oral anticoagulants (DOACs) are seen as equivalent, while in cardiology there is a preference for DOACs.AimTo describe the pattern of oral anticoagulant (OAC) prescribing for AF by GPs and assess whether GPs proactively convert between VKAs and DOACs in patients with AF.Design & settingObservational study using routine practice data from 214 general practices, from 2017 until 2019.MethodPatients aged ≥60 years diagnosed with AF, who had been prescribed OACs by their GPs in 2018 were included. A distinction was made between starters, who were participants who did not use OACs in 2017, and prevalent users. It was observed and recorded whether patients switched between VKAs and DOACs.ResultsA total of 12 516 patients with AF were included. Four hundred and seventy-six patients (4%) started OACs in 2018; 12 040 patients were prevalent OAC users. When GPs started patients on OACs, DOACs were prescribed the most (88%). Among prevalent users, more than half of the patients used VKAs (60%). GPs switched between OACs for 1% of starters and 0.6% of prevalent users in 2018 and 2019.ConclusionDutch GPs predominantly start with DOACs in newly diagnosed patients with AF. Prevalent patients predominantly use VKAs and switching from a DOAC to a VKA is unusual. Consequently, the number of patients using VKAs will decline in the upcoming years. This trend raises questions about the future of organising frequent international normalised ratio (INR) checks for VKA users.
PENGELOLAAN PROGRAM BANK SAMPAH DALAM MENINGKATKAN PEREKONOMIAN KELUARGA PADA BANK SAMPAH INDUK RUMAH HARUM DI KECAMATAN SUKMAJAYA - DEPOK
Authors: Faridah Faridah , Nurlina Nurlina 
Published: January 2023

<p align="justify">This study of the management of the Rumah Harum Main Waste- Bank focuses on the Fragrant House Main Waste Bank's waste management to enhance the economic well-being of families in the Sukmajaya subdistrict – Depok area.The purpose of this research is to ascertain how Rumah Harum's waste bank program is managed in order to support the local families' economies.The qualitative research approach, which is described through elaboration or description based on the facts of the field, was used.The primary waste bank at Rumah Harum is responsible for managing waste-management by collecting it from the community and depositing it there as customers.The manager's passbook serves as the collection's savings account for the Sukmajaya Depok residents.The management of waste itself aims to transform waste into products that can be sold and to assist economically disadvantaged communities.The author draws the conclusion, based on the findings of the analysis, that the management of the waste bank is quite helpful and educates the public about how to choose waste and waste products that are collected and weighed into their savings when needed.However, the issue is that managers still require government assistance to properly manage waste, and the general public is still unaware of this.</p><p><strong>Keywords</strong>: Management, Waste Bank, Family Economy</p>
Over 100 μm thickness CVD diamond film with perfectly aligned nitrogen-vacancy centers on highly misoriented substrates
Authors: Tsuji Takeyuki , Iwasaki Takayuki , Hatano Mutsuko 
Published: January 2023

Abstract
Thick CVD diamond film with perfectly aligned nitrogen-vacancy (NV) centers leads to highly sensitive quantum sensors. A misorientation angle (θ
mis) of the (111) substrate is a key to determine the film thickness. We investigated the dependence of the growth rate and NV center properties on θ
mis(0.4°–9.9°). The growth rate increased from 1.8 to 4.4 μm h−1 and the NV yield (=[NV centers]/[Nitrogen]) increased from 0.4% to 1.1% by increasing θ
mis. Finally, a diamond film of thickness ≈120 μm with aligned NV centers was obtained with an area of 300 μm × 400 μm using θ
mis of 9.9°.
Experimental verification of the Thiele equation for skyrmion Hall angle
Authors: Kim Kitae , Lee Seong-Hyub , Shin Yooleemi , Kim Ji-Wan , Park Jung-Hyun , Chang Jun-Young , Choe Sug-Bong 
Published: January 2023

Abstract
Magnetic skyrmions are a topologically stable spin configurations that are currently extensively studied as promising information carriers in spintronic devices. When an electric current is injected, the skyrmions exhibit the skyrmion Hall effect. This skyrmion Hall effect can be described by the Thiele equation. Here, we experimentally examined the validity of the Thiele equation. For this study, a series of Pt/Co/W films with a wide range of Co layer thicknesses were examined. The results verified the Thiele equation of the skyrmion Hall angle as a function of the magnetic anisotropy and Gilbert damping constants.
Expenditure Food and Nonfood in South Sulawesi Before and During the Covid-19 Pandemi
Authors: Husain Aslim , Martianto Drajat , Ekayanti Ikeu 
Published: January 2023

The impact of the Covid-19 pandemi are disrupting the food security directly such as food system, and indirectly such as household income, food access and health. Examining changes in food and non-food expenditure both before and during the covid-19 epidemic is the goal of this study. In South Sulawesi Province, Indonesia, a descriptive study of both urban and rural areas was employed as the research design. This research uses a cross sectional study research design, the type of data used is secondary data using the 2019-2021 National Socio-Economic Survey data. The results showed that during the pandemic, food expenditure increased, whereas prepared foods, beverages, and cigarette consumption decreased. Different items and services, footwear and hats, as well as party/festival ceremonies, also saw a decrease in non-food expenditure. With a food share of 67,7% in 2020 and 64,3% in 2021, it indicates the presence of food insecurity based on income class (quintiles 1 and 2). In both urban and rural areas, the economic wellbeing level has decreased as a result of the covid-19 pandemic. The conclusion are in South Sulawesi Province the comparison before and during the covid-19 pandemic, food expenditure has increased, non-food expenditure has decreased in 2020 and 2021. People with low incomes are indicated to be food insecure both in urban and rural areas during the Covid-19 pandemic.