bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid and easy construction of a simplified amplicon sequencing (simplified AmpSeq) library for marker-assisted selection
Authors: Nishio Sogo , Moriya Shigeki , Kunihisa Miyuki , Takeuchi Yukie , Imai Atsushi , Takada Norio 
Published: June 2023

AbstractMarker-assisted selection (MAS) is fundamental for plant breeding programs, as it can identify desirable seedlings at a young stage and reduce the cost, time and space needed for plant maintenance, especially for perennial crops. To facilitate the process of genotyping, which is time consuming and laborious, we developed a simplified amplicon sequencing (simplified AmpSeq) library construction method for next-generation sequencing that can be applied to MAS in breeding programs. The method is based on one-step PCR with a mixture of two primer sets: the first consisting of tailed target primers, the second of primers that contain flow-cell binding sites, indexes and tail sequences complementary to those in the first set. To demonstrate the process of MAS using s implified AmpSeq, we created databases of genotypes for important traits by using cultivar collections including triploid cultivars and segregating seedlings of Japanese pear (Pyrus pyrifolia Nakai), Japanese chestnut (Castanea crenata Sieb. et Zucc.) and apple (Malus domestica Borkh.). Simplified AmpSeq has the advantages of high repeatability, ability to estimate allele number in polyploid species and semi-automatic evaluation using target allele frequencies. Because this method provides high flexibility for designing primer sets and targeting any variant, it will be useful for plant breeding programs.
Entropic barrier of water permeation through single-file channels
Authors: Wachlmayr Johann , Fläschner Gotthold , Pluhackova Kristyna , Sandtner Walter , Siligan Christine , Horner Andreas 
Published: June 2023

AbstractFacilitated water permeation through narrow biological channels is fundamental for all forms of life. Despite its significance in health and disease as well as for biotechnological applications, the energetics of water permeation are still elusive. Gibbs free energy of activation is composed of an enthalpic and an entropic component. Whereas the enthalpic contribution is readily accessible via temperature dependent water permeability measurements, estimation of the entropic contribution requires information on the temperature dependence of the rate of water permeation. Here, we estimate, by means of accurate activation energy measurements of water permeation through Aquaporin-1 and by determining the accurate single channel permeability, the entropic barrier of water permeation through a narrow biological channel. Thereby the calculated value for $$\triangle {S}^{{{\ddagger}} }$$
△

S

‡

= 2.01 ± 0.82 J/(mol·K) links the activation energy of 3.75 ± 0.16 kcal/mol with its efficient water conduction rate of ~1010 water molecules/second. This is a first step in understanding the energetic contributions in various biological and artificial channels exhibiting vastly different pore geometries.
Real-time latent heat emission during dynamic-compression freezing of water
Authors: Nissen Erin J. , La Lone Brandon M. , Mance Jason G. , Larson Eric , Dolan Daniel H. 
Published: June 2023

AbstractDynamic compression studies have been used to study the nucleation kinetics of water to ice VII for decades. Diagnostics such as photon Doppler velocimetry, transmission loss, and imaging have been used to measure pressure/density, and phase fraction, while temperature has remained the difficult thermodynamic property to quantify. In this work, we measured pressure/density and implemented a diagnostic to measure the temperature. In doing so the temperature shows quasi-isentropically compressed liquid water forms ice at pressures below the previously defined metastable limit, and the liquid phase is not hypercoooled as previously thought above that limit. Instead, the latent heat raises the temperature to the liquid-ice-VII melt line, where it remains with increasing pressure. We propose a hypothesis to corroborate these results with previous work on dynamic compression freezing. These results provide constraints for nucleation models, and suggest this technique be used to investigate phase transitions in other materials.
Paleobathymetric reconstructions of the SW Barents Seaway and their implications for Atlantic–Arctic ocean circulation
Authors: Lasabuda Amando P. E. , Hanssen Alfred , Laberg Jan Sverre , Faleide Jan Inge , Patton Henry , Abdelmalak Mansour M. , Rydningen Tom Arne , Kjølhamar Bent 
Published: June 2023

AbstractUnravelling past, large-scale ocean circulation patterns is crucial for deciphering the long-term global paleoclimate. Here we apply numerical modelling to reconstruct the detailed paleobathymetry-topography of the southwestern inlet of the Barents Seaway that presently connects the Atlantic and Arctic oceans. Subaerial topography was likely enough to block Atlantic Water from entering the Barents Seaway in the earliest Eocene (c. 55 Ma). The water may have entered in the middle Eocene (c. 47 Ma) as observed from major basin subsidence, but paleotopographic highs to the east may have hindered connections between the two oceans. From the Oligocene (c. 33 Ma) until the onset of the Quaternary (c. 2.7 Ma), basin shallowing and regional shelf uplift blocked Atlantic Water from entering the Barents Seaway. Our results imply that the Fram Strait remained the sole gateway for Atlantic Water into the Arctic Ocean since its opening in the Miocene until the Quaternary.
Master‐slave control strategy of the cascaded multi‐terminal ultra‐high voltage direct current transmission system
Authors: He Sihang , Hao Liangliang , Liu Chang , He Jinghan , Chen Zhengguang 
Published: June 2023

AbstractIn the case of dispersed load consumption, Ultra‐high Voltage Direct Current (UHVDC) system in cascaded connection mode can significantly reduce the cost by building DC lines between different ends, but the communication delay caused by it brings challenges to the original control strategy. Therefore, the control strategy suitable for cascaded multi‐terminal UHVDC (CMT‐UHVDC) system is developed. In this paper, first of all, the operation characteristics, mathematical model, and adaptability of the original control strategy are discussed. Next, an autonomous master‐slave control strategy is proposed to maintain voltage balance without real‐time communication between high‐end and low‐end valves. Finally, based on the planning parameters of an actual project, the electromagnetic transient simulation model of the CMT‐UHVDC transmission system is established. Several working conditions and the practical influence of sampling deviation on the control performance are discussed and analyzed, which verifies the proposed control strategy's effectiveness.
Confronting fuzzy dark matter with the rotation curves of nearby dwarf irregular galaxies
Authors: Bañares-Hernández Andrés , Castillo Andrés , Martin Camalich Jorge , Iorio Giuliano 
Published: June 2023

Aims. In this paper, we carry out a phenomenological investigation of the viability of fuzzy dark matter, which is composed of coherent waves of non-interacting ultralight axions with a mass of ma ≈ 10−22 eV. We did so by confronting the predictions of the model, in particular, the formation of a solitonic core at the center of dark matter halos, with a homogeneous and robust sample of high-resolution rotation curves from the LITTLE THINGS in 3D catalog. This comprises a collection of isolated, dark matter-dominated dwarf-irregular galaxies that provides an optimal benchmark for cosmological studies. Our aim is to find evidence of fuzzy dark matter in the observations; alternatively, we seek to set exclusion ranges for its mass.
Methods. We used a statistical framework based on a χ2 analysis of the rotation curves of the LITTLE THINGS in 3D catalog using a fuzzy dark matter profile as the theoretical model. This allows us to extract relevant parameters such as the axion mass and mass of the solitonic core, as well as the mass of the dark matter halo and its concentration parameter. We fit the data using current Markov chain Monte Carlo techniques with a rather loose set of priors, except for the implementation of a core-halo relation predicted by simulations. The results of the fits were then used to perform various diagnostics on the predictions of the model.
Results. Fuzzy dark matter provides an excellent fit to the rotation curves of the LITTLE THINGS in 3D catalog, with axion masses determined from different galaxies clustering around ma ≈ 2 × 10−23 eV. However, we find two major problems from our analysis. First, the data follow scaling relations of the properties of the core, which are not consistent with the predictions of the soliton. This problem is particularly acute in the core radius-mass relation with a tension that (at face value) has a significance of ≳5σ. The second problem is related to the strong suppression of the linear power spectrum that is predicted by fuzzy dark matter for the axion mass preferred by the data. This can be constrained very conservatively by the galaxy counts in our sample, which leads to a tension that exceeds 5σ. We estimate the effects of baryons in our analysis and discuss whether they could alleviate the tensions of the model with observations.
Mobilidade e resistência muscular lombopélvica e associação com dor musculoesquelética em bailarinas
Authors: Padilha Vitória Hamdan , Lara Simone , Graup Susane , Teixeira Lilian Pinto , Silva Loreanne dos Santos , Maciel Eduardo Timm 
Published: June 2023

ResumoObjetivo Analisar a mobilidade e a resistência muscular lombopélvica e mobilidade de tornozelo, assim como identificar os fatores associados com dor musculoesquelética em bailarinas jovens.Métodos Trata-se de um estudo quantitativo, descritivo e transversal que avaliou 14 bailarinas de 12 a 16 anos. Os seguintes instrumentos foram aplicados: a) Questionário Nórdico de Sintomas Osteomusculares (QNSO) para avaliação da dor musculoesquelética; b) leg lateral reach test, lumbar lock e rotation test (avaliação da mobilidade de tronco) e lunge test (avaliação da mobilidade de tornozelo); c) ponte frontal, extensores lombares e flexores lombares para avaliação da resistência do complexo lombopélvico.Resultados A dor lombar e em membros inferiores, especialmente no joelho (57,1%), foram as principais queixas relatadas pelas bailarinas no presente estudo. As bailarinas avaliadas com dor lombar apresentavam significativamente menor mobilidade lombar (p = 0,05) e menor mobilidade de tornozelo em ambos os lados (p ≤ 0,05). Entre as que apresentavam dores nos joelhos, a resistência muscular de extensores de tronco foi significativamente menor (p = 0,05).Conclusões O presente estudo encontrou associações importantes entre a função do complexo lombopélvico e sintomas musculoesqueléticos e apoia a construção de estratégias preventivas neste contexto.
Polimorfismos genéticos no gene COL1A2 e o risco de tendinopatia: Estudo de caso-controle
Authors: Lopes Lucas Rafael , Guimarães João Antônio Matheus , Amaral Marcus Vinicius Galvão , Pereira Camili Gomes , Wainchtock Victor Soares , Goes Rodrigo Araujo , Miranda Vitor Almeida Ribeiro de , Perini Jamila Alessandra 
Published: June 2023

Resumo
Objetivo Avaliar a influência de polimorfismos nos genes que codificam o colágeno tipo I e a suscetibilidade genética da tendinopatia.
Metodologia Estudo caso-controle envolvendo 242 atletas brasileiros de diferentes modalidades esportivas (55 casos de tendinopatia e 187 controles). Os polimorfismos COL1A1 (rs1107946) e COL1A2 (rs412777, rs42524 e rs2621215) foram analisados pelo sistema TaqMan. As razões de chance (OR) com seus intervalos de confiança (IC) de 95% foram calculadas usando um modelo de regressão logística não-condicional.
Resultados A média de idade foi de 24,0 ± 5,6 anos e 65,3% eram homens. Dos 55 casos de tendinopatia, 25,4% apresentaram mais de um tendão acometido, sendo os mais frequentes o patelar (56,3%), o manguito rotador (30,9%) e o do cotovelo ou flexores das mãos (30,9%). A idade e o tempo de prática esportiva foram associados a uma maior chance de apresentar tendinopatia (5 e 8 vezes, respectivamente). A frequência dos alelos variantes nos controles e casos, respectivamente, foi: COL1A1 rs1107946 24,0 e 29,6%; COL1A2 rs412777 36,1 e 27,8%; rs42524 17,5 e 25,9%; e rs2621215 21,3 e 27,8%. Após ajuste pelos fatores de confundimento (idade e anos de práticas esportiva), os polimorfismos COL1A2 rs42524 e rs2621215 foram associados a um risco aumentado de tendinopatia (OR = 5,5; IC95% = 1,2–24,6 e OR = 3,9; IC95% = 1,1–13,5, respectivamente). O haplótipo COL1A2
CGT foi associado a um baixo risco para desenvolvimento da doença (OR = 0,5; IC95% = 0,3–0,9).
Conclusão A idade (≥ 25 anos), o tempo de prática esportiva (≥ 6 anos) e polimorfismos no gene COL1A2 aumentaram o risco de desenvolvimento da tendinopatia.
Análise de propriocepção por estabilometria após a reconstrução do ligamento cruzado anterior: Um estudo prospectivo e longitudinal
Authors: Al-Dadah Oday , Shepstone Lee , Donell Simon T. 
Published: June 2023

Resumo
Objetivo Os métodos comumente usados para medir a propriocepção resultaram em relatos conflitantes sobre a propriocepção do joelho com ruptura do ligamento cruzado anterior (LCA) e a influência da reconstrução do LCA.
Métodos A propriocepção de 100 indivíduos (50 pacientes com ruptura unilateral do LCA confirmada à radiologia e artroscopia e 50 controles normais) foi avaliada por estabilometria postural dinâmica em apoio unipodal. A lassidão ligamentar do joelho instrumentado e suas pontuações de desfechos também foram medidas. Dos 50 pacientes do grupo LCA, 34 foram submetidos à reconstrução e reavaliados no período pós-operatório.
Resultados O grupo LCA apresentou deficiência proprioceptiva significativa em relação ao joelho contralateral (p < 0,001) e ao grupo controle (p = 0,01). A propriocepção do joelho melhorou de maneira significativa após a reconstrução do LCA em relação aos achados pré-operatórios (p = 0,003). Não houve correlação entre as medidas de lassidão ligamentar e as pontuações de desfechos. Além disso, observamos uma correlação significativa entre as pontuações de desfechos e as medidas de propriocepção antes da cirurgia. Essa correlação não foi detectada no período pós-operatório. O teste pré-operatório de propriocepção teve correlação significativa (r = 0,46) com a propriocepção pós-operatória (p = 0,006).
Conclusão Os pacientes com ruptura do LCA apresentaram déficit proprioceptivo que melhorou após a reconstrução ligamentar. As pontuações de desfecho do joelho tiveram melhor correlação à propriocepção do que a lassidão ligamentar. A propriocepção pode ser uma medida objetiva superior à lassidão ligamentar na quantificação de déficits funcionais e desfechos do joelho em pacientes com ruptura do LCA.
Nível de Evidência III Estudo Terapêutico; Estudo de Caso-Controle Longitudinal Prospectivo.
Osteointegração e sucesso em revisão acetabular de artroplastia total de quadril com enxerto estrutural homólogo: seguimento médio de 9,6 anos
Authors: Roos Bruno Dutra , Roos Milton Valdomiro , Camisa Antero , Lima Ezequiel Moreno Ungaretti , Betto Maurício Domingos , Dubiela Rafaela Scuzziato 
Published: June 2023

Resumo
Objetivo Avaliar os resultados clínicos, radiográficos e a sobrevida da cirurgia de revisão acetabular de artroplastia total de quadril com implante cimentado sem uso de anel de reforço, associado à enxertia óssea homóloga estrutural.
Métodos Um total de 40 pacientes (44 quadris) operados de 1995 a 2015 foram analisados retrospectivamente. As radiografias foram avaliadas de acordo com a classificação do defeito ósseo acetabular, o formato do enxerto e à presença de osteointegração. Foram considerados casos de insucesso a migração do implante > 5mm em qualquer direção e/ou a progressão de linhas de radioluscência em torno do componente acetabular > 2mm. Verificamos a associação dos achados radiográficos com os casos de falha utilizando testes estatísticos e analisamos a sobrevida utilizando a curva de Kaplan-Meier.
Resultados Dos 44 quadris, 45,5% dos defeitos acetabulares eram Paprosky tipo 3A e 50%, 3B. Em 65% dos quadris, a configuração do enxerto foi classificada como tipo 1 de Prieto e em 31% como tipo 2. Não foi observada evidência radiográfica de osteointegração em 13,6% dos casos. Observamos 9 (20,5%) falhas de reconstrução. Foi observada correlação entre falha da reconstrução com a ausência de sinais radiográficos de osteointegração do enxerto.
Conclusão Observamos bons resultados clínicos e radiográficos, com sobrevida de 79,54% em seguimento médio de 9,65 anos. Também houve associação entre ausência de sinais radiográficos de osteointegração do enxerto estrutural e falha nesta série de pacientes com grandes defeitos ósseos. As falhas não se correlacionaram com a severidade do defeito ósseo acetabular, espessura ou configuração do enxerto.
Circumbilical Access for Ventriculoperitoneal Bypass Insertion in Adults
Authors: Silva Vithor Ely Bortolin da , Rodrigues José Carlos 
Published: June 2023

Abstract
Introduction The use of a minilaparotomy for catheter implantation can bring important complications such as adhesions, intestinal lesions, incisional hernias and postoperative pain. In neurosurgery, the umbilical access, currently widely used by surgeons of different specialties mainly for its aesthetic results, is still restricted by the unfamiliarity of the access.
Material and Method During the period between 2019 and 2020, a total of 12 patients who required ventricular bypass were selected, using circumbilical access for insertion of the peritoneal catheter and followed up for 12 months to analyze possible complications.
Description of the Technique The surgeon responsible for the abdomen performs an umbilical incision bordering the upper edge of the upper ring, avoiding the mamelon, quickly finding the linea alba under the umbilical plane, which after dissection allows reaching the peritoneum, without breaking the rectus muscles. The peritoneum can then be opened under visual control.
Results All patients presented resolution of hydrocephalus with good aesthetic results and without complications.
Discussion The aesthetic result of the transumbilical procedure was the stimulus for the development of the technique that proved to be easy, safe, cheap, and aesthetic. Initially, the ease of access to the peritoneal cavity is clear, in addition to avoiding manipulation of the rectus abdominis muscle, which improves postoperative pain.
Conclusion The circumbilical access for the implantation of a ventriculoperitoneal shunt is safe, effective and has a better aesthetic result for adult patients and should be part of the operative arsenal of neurosurgeons.
Zhang Zhongjing Cultural Brand Construction from the Perspective of Traditional Chinese Medicine Culture and Wellness Tourism
Authors: Xu Erping , Liu Baoguang , Bai Ming , Liu Yalin , Li Yucheng , Wang Baoying , Chen Yiheng , Xie Miao , Dong Ying 
Published: June 2023

AbstractNowadays, traditional Chinese medicine (TCM) culture and wellness tourism are developing toward integration with an inherent and inevitable basis. A new business mode of TCM wellness tourism bred has emerged, injecting new vitality into the development of TCM culture and innovation of tourism. As the cultural representative of TCM industry, Zhang Zhongjing culture should seize the opportunity to make a breakthrough in its brand construction with the help of wellness tourism. The main construction strategies include actively launching books or films and television dramas based on the deeds of Zhang Zhongjing and other famous TCM doctors, turning herbs cultivation bases into special tourism and sightseeing parks, establishing research and dissemination bases of TCM culture, and building TCM wellness tourism bases that integrate learning, rehabilitation, and tourism, using information technology and cultural media platforms to introduce Zhang Zhongjing culture to the world.
Association between Peripartum Mean Arterial Pressure and Postpartum Readmission for Preeclampsia with Severe Features
Authors: Lin Bing-Xue , Smith Maria , Sutter Megan , Penfield Christina A. , Proudfit Christine 
Published: June 2023

Objective This study aimed to evaluate the relationship between peripartum mean arterial pressure (MAP) and postpartum readmission for preeclampsia with severe features.
Study Design This is a retrospective case–control study comparing adult parturients readmitted for preeclampsia with severe features to matched nonreadmitted controls. Our primary objective was to evaluate the association between MAP at three time points during the index hospitalization (admission, 24-hour postpartum, and discharge) and readmission risk. We also evaluated readmission risk by age, race, body mass index, and comorbidities. Our secondary aim was to establish MAP thresholds to identify the population at highest risk of readmission. Multivariate logistic regression and chi-squared tests were used to determine the adjusted odds of readmission based on MAP. Receiver operating characteristic analyses were performed to evaluate risk of readmission relative to MAP; optimal MAP thresholds were established to identify those at highest risk of readmission. Pairwise comparisons were made between subgroups after stratifying for history of hypertension, with a focus on readmitted patients with new-onset postpartum preeclampsia.
Results A total of 348 subjects met inclusion criteria, including 174 controls and 174 cases. We found that elevated MAP at both admission (adjusted odds ratio [OR]: 1.37 per 10 mm Hg, p < 0.0001) and 24-hour postpartum (adjusted OR: 1.61 per 10 mm Hg, p = 0.0018) were associated with increased risk of readmission. African American race and hypertensive disorder of pregnancy were independently associated with increased risk of readmission. Subjects with MAP > 99.5 mm Hg at admission or >91.5 mm Hg at 24-hour postpartum had a risk of at least 46% of requiring postpartum readmission for preeclampsia with severe features.
Conclusion Admission and 24-hour postpartum MAP correlate with risk of postpartum readmission for preeclampsia with severe features. Evaluating MAP at these time points may be useful for identifying women at higher risk for postpartum readmission. These women may otherwise be missed based on standard clinical approaches and may benefit from heightened surveillance.
Key Points
Transition in the societal value and governance of water resources in Australia and China
Authors: Wei Jing , Wei Yongping , Tian Fuqiang , Xiong Yonglan , Hu Hongchang 
Published: June 2023

AbstractThe transition of a country or a whole society to sustainability is a long-term goal that involves a society’s willingness to carry out such a change and the governance structure to support it. Understanding the role of the governance structure in enabling or constraining the change dynamics of societal values can more effectively foster desired transitions. This paper aims to reveal the co-evolutionary dynamics in the transition processes of the societal value of water resources and the network configurations of water governance in Australia and China in different socioecological contexts. Newspapers were used to track societal value and structural change in water governance. One mainstream newspaper was selected from each country, spanning a timeframe of 175 years in Australia and 72 years in China. The key finding is that the transition in societal value in both countries followed a similar sigmoid function. The value transition process in both countries benefited from a centralized governance structure, particularly in the take-off stage of the transition.