bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of factors influencing the intravertebral shell phenomenon after posterior reduction internal fixation of thoracolumbar fracture: A case-control study
Authors: Fang Yao , Chen Wugui , Zhang Sining , Ye Yuchao , Chen Kongning , Ling Guangfeng , Wang Qing , Liu Chengzhao 
Published: June 2023

Abstract
Background: To investigate the factors influencing the intravertebral shell phenomenon (ISP) after posterior repositioning internal fixation of thoracolumbar fractures and the related factors of evolutionary outcomes and to make recommendations for the prevention and treatment of this phenomenon.
Methods: We retrospectively analysed 331 patients with single-segment fractures of the thoracolumbar vertebrae treated with posterior repositioning internal fixation using the posterior pedicle nail system from January 2016 to December 2020. Univariate and multivariate logistic regressions were applied to analyse the factors influencing the ISP.
Results: A total of 260 patients (78.5%) were found to have the postoperative ISP. Reduced bone density, preoperative vertebral over-compression, and intraoperative vertebral over-sparing were significantly correlated with the occurrence of postoperative ISP(P<0.05). A total of 166 of the 260 patients were reviewed by CT 1 year after surgery, revealing reduction or healing of the vertebral cavity in 104 patients (62.6%) and enlargement of the vertebral cavity or collapse of the endplate in 62 patients (37.4%). The degree of intraoperative vertebral body repositioning was strongly correlated with the outcome of the intravertebral shellphenomenon (P<0.05). A total of 123 patients (74%) had their internal fixation devices removed 1 year after surgery, which was significantly correlated withthe degree of intraoperative vertebral body repositioning and the outcome of the ISP 1 year after surgery (P<0.05).
Conclusion: The ISP occurs commonly after internal fixation of thoracolumbar fractures and is associated with reduced bone density and excessive preoperative compression intraoperative sparing of the vertebrae. At 1 year postoperatively, 37.4% of patients still had enlarged vertebral cavities or endplate collapse, which affected patient access to internal fixation; the impact on patient function needs further study.
Analysis of drought and heat stress response genes in rice using co-expression network and differentially expressed gene analyses
Authors: Cao Gaohui , Huang Hao , Yang Yuejiao , Xie Bin , Tang Lulu 
Published: June 2023

Abstract

Studies on Oryza sativa (rice) are crucial for improving agricultural productivity and ensuring global sustenance security, especially considering the increasing drought and heat stress caused by extreme climate change. Currently, the genes and mechanisms underlying drought and heat resistance in rice are not fully understood, and the scope for enhancing the development of new strains remains considerable. To accurately identify the key genes related to drought and heat stress responses in rice, multiple datasets from the Gene Expression Omnibus (GEO) database were integrated in this study. A co-expression network was constructed using a Weighted Correlation Network Analysis (WGCNA) algorithm. We further distinguished the core network and intersected it with differentially expressed genes and multiple expression datasets for screening. Differences in gene expression levels were verified using quantitative real-time polymerase chain reaction (PCR). OsDjC53, MBF1C, BAG6, HSP23.2, and HSP21.9 were found to be associated with the heat stress response, and it is also possible that UGT83A1 and OsCPn60a1, although not directly related, are affected by drought stress. This study offers significant insights into the molecular mechanisms underlying stress responses in rice, which could promote the development of stress-tolerant rice breeds.
The Evolution of Atomistic Structure and Mechanical Property of Coke in the Gasification Process with CO2 and H2O at Different Temperatures: a ReaxFF molecular dynamics study
Authors: Xiong Zixin , Bu Yushan , Liang Zeng , Li Kejiang , Zhang Hang , Liao Haotian , Zhou Feng , Zhang Jianliang 
Published: June 2023

Abstract
Molecular dynamics simulations with reactive force-field (ReaxFF-MD) were performed to construct the molecular model of coke. The coke was placed in a mixed atmosphere of H2O/CO2 with different concentration ratios to investigate the dissolution loss reaction. It was found that different atmospheric concentrations had different effects on the coke model structure and reaction sites. The CO2 molecular tended to dissolve on the surface of coke and destroyed its surface structure, while the H2O molecular was more likely to enter the coke to destroy its structure, which was consistent with the experimental results. At the same time, the tensile simulation of the coke model after the reaction was carried out to observe the changes in the mechanical properties of the structure after the reaction. It was found that CO2 and H2O have varying degrees of influence on the mechanical properties of the coke model. Controlling the composition content of the reaction gas can effectively adjust the tensile resistance of the coke model. It was also found that different temperatures have an impact on the mechanical properties of the coke model, and the tensile resistance of the coke was poor at high temperatures. By revealing the behavior of coke model at the micro scale, it provides a theoretical basis for the industrial coke application process.
Analogue of Ramanujan's function k(τ) for the continued fraction of order six
Authors: Guadalupe Russelle , Aricheta Victor Manuel 
Published: June 2023

Abstract
We study the modularity of the function w(τ ) = X(τ )X(3τ ), where X(τ ) is the continued fraction of order six introduced by Vasuki, Bhaskar and Sharath (2010). The function w(τ ) is an analogue of Ramanujan’s function k(τ ) = r(τ )r(2τ )2 , where r(τ ) is the Rogers-Ramanujan continued fraction. We prove that w(τ ) is an η-quotient that generates the field of all modular functions on the congruence subgroup Γ0(18), and express X(τ ) and X(3τ ) in terms of w(τ ). We also show that there is a modular equation for w(τ ) of any level, and provide explicitly the modular equations of level p for primes p ≤ 13. We finally show that w( τ/3 ) generates the ray class field K(3) over an imaginary quadratic field K modulo 3 for some suitable τ ∈ K.
Fabrication of lidocaine-loaded polymer dissolving microneedles for rapid and prolonged local anesthesia
Authors: Mao Yanan , Zhang Xiufeng , Sun Yanfang , Shen Zhong , Zhong Chao , Nie Lei , Shavandi Amin , Yunusov Khaydar E. , Jiang Guohua 
Published: June 2023

Abstract
There is an urgent need for research on effective interventions for pain management to improve their life quality. Traditional needle and syringe injection were used to administer the local anesthesia. However, it causes various discomforts, ranging from brief stings to trypanophobia and denial of medical operations. In this study, a dissolving microneedles (MNs) system made of composite matrix materials of polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), and sodium hyaluronate (HA) was successfully developed for the loading of lidocaine hydrochloride (LidH). The morphology, size and mechanical properties of the MNs were also investigated. After the insertion of MNs into the skin, the matrix at the tip of the MNs was rapidly dissolved, releasing the loaded LidH to diffuse into deeper skin tissue through microchannels formed by MNs insertion. The back patching of MNs could be acted as a drug reservoir to form a prolonged local anesthesia effect due to the swelling and dissolving of MNs by absorption of interstitial fluid. The results showed that LidH MNs provided a superior analgesia up to 8 h, exhibiting a rapid and long-lasting analgesic effects. Additionally, tissue sectioning and in vitro cytotoxicity tests indicated that the MNs patch we developed had a favorable biosafety profile.
Understanding Stim-1 Regulatory Networks in Nasopharyngeal Cancer Using Functional, Transcriptomics, Proteomics, and in-silico Approaches
Authors: Mydin Rabiatul Basria SMN , Azlan Adam , Gooderham Nigel J. , Mohamed Nur Mazidah Haji Noor , Okekpa Simon I. , Moses Emmanuel Jairaj , Amran Syazwani Itri , Musa Muhammad Yusri 
Published: June 2023

Abstract
Objective
Stromal-interaction molecule 1 (STIM-1) aberrant expression contributes significantly to cancer pathogenesis. The present work studied STIM-1 regulatory networks in nasopharyngeal cancer (NPC) models via functional, transcriptome, translational, and in-silico approaches.
Methods
STIM-1 knockdown models were established in Epstein–Barr virus (EBV) related NPC cells (C666-1) and non-EBV related NPC cells (HK-1) respectively using DsiRNA technology. At the functional level, STIM-1 knockdown showed that it could regulate NPC oncogenic state possibly via calcium and ROS activities, especially in EBV-related NPC models. STIM-1 knockdown could inhibit EBV-related-NPC cells efficiently as compared to non-EBV-NPC cells. Regulation at transcriptomic and translation was observed in four critical regulatory networks involving PI3K/Akt, cell cycle, calcium signaling, and apoptosis.
Results
The PI3K/AKt pathway was found to be affected by the STIM-1 network in NPC through the association of miR-375, miR-185-5p, miR 200a-3p, let-7b-5p, and miR-34a-5p. In silico work support these findings, where miR-200a-3p and miR-375 docking scores showed a high probability of binding with STIM-1 and Epstein–Barr virus nuclear antigen 1 (EBNA-1) networks.
Conclusion
This study also found that NPC stemness was affected by STIM-1 suppression. Further analysis suggested STIM-1 regulatory networks have a positive correlation with the EBV status in NPC pathogenesis. Further work on NPC clinical samples is needed to support this work, especially in understanding the therapeutic potential of STIM-1 in NPC.
The relevance of the non-invasive biomarkers lncRNA GAS5/miR- 21 ceRNA regulatory network in the early identification of diabetes and diabetic nephropathy
Authors: Sun He , Chen Tong , Li Xin , Zhu Youyong , Zhang Shuang , He Ping , Peng Yali , Fan Qiuling 
Published: June 2023

Abstract
Background
To investigate the diagnostic value of serum lncRNA growth arrest-specific transcript 5 (lncRNA GAS5) and microRNA-21 (miR-21) in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN), and elucidate their roles in the pathogenesis.
Methods
A microarray technology was used asses lncRNA GAS5 and miR-21 expression profiles in non-anticoagulant blood from 44 patients including T2DM without DN group (DM), T2DM with DN group (DN), and healthy controls group (N), followed by real-time PCR validation. Logistic regression and receiver operating characteristic (ROC) curves were applied to evaluate the clinical indicators among normal, T2DM, and DN patients.
Results
The serum lncRNA GAS5 expression in T2DM and DN patients was significantly down-regulated compared with the N group, while the expression of miR-21 was significantly up-regulated (all P < 0.05). Fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c) were negatively correlated with serum lncRNA GAS5, and FBG was independently correlated with serum lncRNA GAS5. Urinary microalbumin, total cholesterol (TC), creatinine (Cr), urea, and systolic blood pressure (SBP) were significantly positively correlated with serum miR-21. Glomerular filtration rate (GFR) and albuminuria (ALB) were negatively correlated with serum miR-21, and ALB was independently correlated with serum miR-21. Serum lncRNA GAS5, miR-21 and lncRNA GAS5/miR-21 showed good diagnostic efficiency as the "diagnostic signature" of T2DM and DN.
Conclusion
The lncRNA GAS5/miR-21 diagnostic signature may be a more effective non-invasive biomarker for detecting T2DM. In addition, miR-21 alone may be a more accurate serum biomarker for the early screening of DN patients.
MSCs-derived exosomes containing miR-486-5p attenuates cerebral ischemia and reperfusion (I/R) injury through inhibiting PTEN
Authors: Zhu Genbao , Jiang La , Tan Kemeng , Li Yafen , Hu Mengxue , Zhang Shengnan , Liu Zhenlin , Li Lili 
Published: June 2023

Abstract

Objectives
Our study,s aim is to investigate the impact of MSCs-exosomes on cerebral ischemia and reperfusion (I/R) injury, and the underlying mechanism.
Methods
Animal model with cerebral ischemia was established by middle cerebral artery occlusion (MCAO), and cell model of Neuro-2a cells was constructed by oxygen-glucose deprivation/reoxygenation (OGD/R). Exosomes (Exo) derived from mesenchymal stem cells (MSCs) of mice were used to inject into mice or stimulate Neuro‐2a cells. Exosomes from MSCs transfected with miR-NC, miR-486-5p mimics, miR-486-5p inhibitor, or sh-PTEN were used to stimulate Neuro‐2a cells. The regulatory axis of miR-486-5p and PTEN was confirmed by rescue experiments.
Results
Exo-miR-486-5p mimics improved cerebral I/R injury caused neurological deficits and infarct ration, and also attenuated cell apoptosis in vivo. Exo-miR-486-5p mimics notably attenuated OGD/R induced defect on cell viability and inhibited apoptosis of Neuro-2a cells in vitro. Exo-miR-486-5p mimics reduced level of LDH and MDA, while enhanced SOD activity both in brain tissues homogenate of mice and cell supernatant. Mechanically, PTEN was a target of miR-486-5p, and downregulation of PTEN obviously elevated Exo-miR-486-inhibitor caused reduction on cell viability, and reduced it induced elevation on cell apoptosis.
Conclusion
Our results demonstrated exosomes derived from MSCs might protect against cerebral I/R injury, through miR-486-5p and PTEN axis.
Nuclear genome-encoded mitochondrial OXPHOS complex I genes in Buffalo show tissue-specific differences
Authors: E.M Sadeesh , Lahamge Madhuri S , M Anuj , A.N Ampadi 
Published: June 2023

Abstract
Background
Buffaloes' energy status is a vital attribute influencing their phenotypic traits and overall health. Mitochondria, primarily through oxidative phosphorylation (OXPHOS), contribute significantly to energy generation; both nuclear (nDNA) and mitochondrial (mtDNA) genomes are involved in OXPHOS process. Previous studies from our laboratory have reported tissue heterogeneity in buffaloes, particularly in mitochondrial functional attributes, is influenced by the mtDNA. Furthermore, there is evidence of higher OXPHOS complex I activity and expression of OXPHOS complex I genes encoded by the mtDNA in various buffalo tissues. Complex I is the largest and mostly involved in energy generation and maintenance of reactive oxygen species. This largest OXPHOS complex consists of proteins encoded by both nDNA and mtDNA. Currently, the tissue-specific expression of nDNA encoded OXPHOS complex I genes expression in metabolically active tissues of buffalo are not well understood. Therefore, the study aimed to investigate the tissue-specific expression of nDNA-encoded OXPHOS complex I genes in buffaloes.
Methods and Results
To analyze the expression of the OXPHOS complex I genes encoded by nDNA across the various tissues to gain insight into tissue-specific diversity in energy metabolism, RNA-Seq was performed on total RNA extracted from kidney, heart, brain, and ovary of four buffaloes, subsequently identified differentially expressed genes (DEGs) in various tissues comparison. Out of 57 identified OXPHOS complex I genes encoded by nDNA, 51 genes were found to be expressed in each tissue. Comparative analysis revealed 12 DEGs between kidney and brain, 30 for kidney vs ovary, 26 for kidney vs heart, 20 for heart vs brain, 38 for heart vs ovary, and 26 for brain vs ovary, with log2(FC)≥1 and p<0.05. Notably, compared to the ovary, other tissues such as the heart, kidney cortex, and brain exhibited a higher proportion of up-regulated OXPHOS complex I genes. The finding of nuclear derived OXPHOS complex I genes expression of our study showed a close relation with our earlier published report from our laboratory concerning OXPHOS complex I activity.
Conclusions
Our findings revealed substantial changes in OXPHOS complex I subunit gene expression encoded by nDNA across tissues, with up-regulation of specific genes potentially reflecting increased metabolic needs or adaptation to specific roles. These tissue-specific differential expression patterns of OXPHOS complex I subunit-related genes provide valuable insights into the importance of their integrity for tissue-specific energy requirements, mitochondrial function, and their implications for buffalo's productive and reproductive health.
Unlocking the Potential of Micromonospora sp.: Metabolomics and Molecular Modeling to Combat Antibiotic Resistance in Enteric Pathogens via ATP Synthase Inhibitors
Authors: El-Shatoury Sahar A. , Megawer Hanan K. , Nafie Mohamed S. , Makharita Rabab R. , Riesco Raúl , Trujillo Martha E. , Hanora Amro 
Published: June 2023

Abstract
Background
The misuse of antibiotics in the last decade led to the quick emergence of antibiotic resistance among pathogenic microorganisms. It has become a serious health problem that motivates researchers to explore new antimicrobial compounds. Micromonospora is one of the most important producers of commercially successful secondary bioactive metabolites, especially antibiotics such as aminoglycosides, enediynes, and oligosaccharides. This study highlights the potential of Micromonospora spp. as a source of novel antibacterial agents against multidrug resistant human enteric pathogens.
Results
The metabolic extract from Micromonospora sp. 65SH showed the lowest MIC against Enterobacter sp. (25 µg/ml) and E. coli (12.5 µg/ml). We performed 16S rRNA gene sequencing to confirm the isolate’s identification at the genus level. Non-targeted metabolomics and molecular LC-QTOF-MS-MS modeling technique were used to identify potential compounds, mechanisms of action and target sites for new antibiotics derived from Micromonospora sp 65SH. The non-targeted metabolomics and molecular modeling of strain 65SH have shown six inhibitors to interfere with the E. coli ATP synthase and possible direct inhibitory effect on the ATP-dependent bacterial topoisomerases. These are: Melibiose, Oligomycin A, Queuine, Heptelidic acid, Diethyl phthalate, and 2'-Deoxyguanosine.
Conclusion
This research on Micromonospora metabolites holds promise for identifying unique compounds, understanding their mechanisms of action, and ultimately developing innovative strategies to target ATP synthase and address various diseases or conditions associated with aberrant energy metabolism. As scientists delve deeper into this relatively unexplored area, the future holds exciting prospects for the discovery and development of Micromonospora-derived ATP synthase inhibitors to combat antibiotic resistance.
Synthesis of MnO 2 electrode material by electro-deposition method for the enhanced capacitive properties
Authors: Patil S. S. , Patil V. L. , Tawade A. K. , Sharma K. K. , Bhosale A. G. , Vhanalkar S.A 
Published: June 2023

Abstract
Supercapacitor is an alternative for the conventional battery source owing to the safety, electrochemical stability, high power density, short charging time and long cycle life. In this view, MnO2 as an electrode material was synthesized via electrodeposition method to produce MnO2 thin films on stainless steel substrates as an electrochemical supercapacitors. The various characterization techniques were employed to study their physico-chemical properties. The scanning electron microscopy reveals interconnected nanowalls-like morphology of MnO2 thin films, which offers a higher surface area. As a function of the surface morphology, the synthesized MnO2 thin film-based capacitor showed highest specific capacitance of 464.45 Fg-1 at a low scan rate of 10mV.s-1 and galvonostatics charge discharge specific capacitance of 284.05 Fg-1 for current density of 1mA/cm2. In addition, the MnO2 based supercapacitor has also shown the higher cyclic stability at about 98% even after 500 cyclic voltammetry test.
Healthcare provider and drug dispenser knowledge and adherence to guidelines for the case management of malaria in pregnancy in the context of multiple first-line artemisinin-based combination therapies in Western Kenya
Authors: Osoro Caroline B. , Dellicour Stephanie , Ochodo Eleanor , Young Taryn , Kuile Feiko ter , Gutman Julie R. , Hill Jenny 
Published: June 2023

Abstract
Background
Concerns about emerging resistance to artemether-lumefantrine (AL) in Africa prompted the pilot introduction of multiple first-line therapies (MFT) in Western Kenya, potentially exposing women-of-childbearing-age (WOCBA) to antimalarials with unknown safety profiles in the first trimester. We assessed healthcare provider knowledge and adherence to national guidelines for managing malaria in pregnancy in the context of the MFT pilot.
Methods
From March to April 2022, we conducted a cross-sectional study in 50 health facilities (HF) and 40 drug outlets (DO) using structured questionnaires to assess pregnancy detection, malaria diagnosis, and treatment choices by trimester. Differences between HF and DO providers and between MFT and non-MFT HFs were assessed using Chi-square tests.
Results
Of 174 providers (77% HF, 23% DO), 56% were from MFT pilot facilities. Most providers had tertiary education; 5% HF and 20% DO had only primary or secondary education. More HF than DO providers had knowledge of malaria treatment guidelines (62% vs 40%, p=0.023), received training in malaria in pregnancy (49% vs 20%, p=0.002), and reported assessing for pregnancy in WOCBA (98% vs 78%, p<0.001). Most providers insisted on parasitological diagnosis, with 59% HF using microscopy and 85% DO using rapid diagnostic tests. More HF than DO providers could correctly name the drugs for treating uncomplicated malaria in the first trimester (oral quinine, or AL if quinine is unavailable) (90% vs 58%, p<0.001), second and third trimesters (artemisinin-based combination therapies) (84% vs 70%, p=0.07), and for severe malaria (parenteral artesunate/artemether) (94% vs 60%, p<0.001). Among HF providers, those in the MFT pilot had more knowledge of malaria treatment guidelines (67% vs 49%, p=0.08) and had received training on treatment of malaria in pregnancy (56% vs 32%, p=0.03). Few providers (10% HF and 12% DO) had adequate knowledge of malaria treatment in pregnancy, defined as the correct drug and dose for uncomplicated and severe malaria in all trimesters.
Conclusions
Knowledge of national malaria in pregnancy treatment guidelines among providers in western Kenya is suboptimal. Robust training on appropriate antimalarial and dosage is needed. Supervision of DO and HF practices is essential for correct treatment of malaria in pregnancy in the context of MFT programmes.
An improved SMOTE based on center offset factor and synthesis strategy for imbalanced data classification
Authors: Zhang Ying , Deng Li , Huang Hefeng , Wei Bo 
Published: June 2023

Abstract
It is an enormous challenge for imbalanced data learning in the field of machine learning. To construct balanced datasets, oversampling techniques have been studied extensively. However, many oversampling methods suffer from introducing noisy samples and blurring classification boundaries, which leading to overfitting. To solve this problem, this paper proposes a new oversampling method, namely CS-SMOTE, for synthesizing minority class samples by three-point interpolation. CS-SMOTE is mainly based on the center offset factor and a synthesis strategy. First, the CS-SMOTE method removes noise samples, calculates the center offset factor, and selects sparsely distributed minority class samples by using the K-distance graph technique. Next, new samples are generated basing on sparse minority samples, random minority samples, and sub-cluster centers located in the same sub-cluster samples. Finally, multiple comparative experiments on 18 well-known datasets demonstrate the effectiveness and general applicability of the proposed CS-SMOTE method for the imbalanced data classification. The experiments show that CS-SMOTE outperforms other competitors in terms of classification accuracy, while avoiding the issue of overfitting.