bluuebunny/crossref_metadata_2025_split huggingface dataset:
KaiA regulates intracellular oxygen dynamics in unicellular diazotrophic cyanobacteria
Authors: Bandyopadhyay Anindita , Sengupta Annesha , Pakrasi Himadri 
Published: March 2023

Abstract

The discovery of nitrogen fixation in unicellular cyanobacteria provided the first clues for the existence of a circadian clock in prokaryotes. However, recalcitrance to genetic manipulation barred their use as model systems for deciphering the clock function. Here, we explore the circadian clock in the now genetically amenable Cyanothece 51142, a unicellular, nitrogen-fixing cyanobacterium. Unlike non-diazotrophic clock models, Cyanothece 51142 exhibits conspicuous self-sustained rhythms in various discernable phenotypes, offering a platform to directly study the effects of the clock on the physiology of an organism. Deletion of kaiA in this strain impacted regulation of oxygen cycling and hindered nitrogenase activity. Our findings imply a role for KaiA in regulating the intracellular oxygen dynamics in unicellular diazotrophic cyanobacteria and suggest that its addition to the KaiBC clock was likely an adaptive strategy that ensured optimal nitrogen fixation as microbes evolved from an anaerobic to an aerobic atmosphere under nitrogen constraints.
Recommendations for robust renovation strategies of Swiss residential buildings
Authors: Galimshina Alina , Moustapha Maliki , Hollberg Alexander , Lasvaux Sébastien , Sudret Bruno , Habert Guillaume 
Published: March 2023

Abstract
Building renovation is urgently required to reduce the environmental impact associated with the building stock. Typically, building renovation is performed by envelope insulation and/or changing the fossil-based heating system. Life cycle assessment and life cycle cost analysis are seen as suitable methods to evaluate the performance of a building in terms of environmental impacts and cost. However, the results are highly affected by uncertainty. The goal of this paper is to provide recommendations for robust renovation strategies considering uncertainties on future evolution of climate, energy grid, user behaviors. The method includes identifying optimal renovation options for the envelope and heating systems for building-representatives from all construction periods that are currently in need of retrofit in Switzerland. Conventional and bio-based materials are examined with inclusion of dynamic carbon storage potential. The results of the analysis show that heating system is a priority for building renovation for all the considered construction periods. The results also show that if only conventional materials are considered for building renovation, optimal solution does not prescribe deep renovation and higher energy consumption during life time is favored. However, when bio-based insulation materials can be used, the opposite conclusion is drawn and synergies between low environmental impact and low energy costs during life cycle are identified. Such renovation strategies pave the way for a just and low carbon transformation of the Swiss building stock.
Ultrafast inactivation of SARS-CoV-2 by 254-nm UV-C irradiation on porous and non-porous media of medical interest using an omnidirectional chamber
Authors: Maubert Bertrand , Theillère Camille , Jany Prescillia , Bourlet Thomas , Deschamps Jérôme , Pozzetto Bruno , Singh Fateh , Gadea Emilie 
Published: March 2023

Abstract
Covid-19 has spurred a renewed interest at decontamination techniques for air, objects and surfaces. Beginning 2020, a lot of effort was done in urgency to permit the reuse of UC-V for inactivating SARS-CoV-2. However, those studies diverged widely on the dose necessary to reach this goal; until today, the real value of the sensitivity of the virus to a 254-nm illumination is not known precisely. In this study, decontamination was performed in an original UV-C large decontamination chamber (UVCab, ON-LIGHT, France) delivering an omnidirectional irradiation with an average dose of 50 mJ/cm2 in 60 sec. Viral inactivation was checked by both cell culture and PCR test. SARS-CoV-2 was inactivated by UV-C light within 3 sec on both porous (disposable gown) and non-porous (stainless steel and apron) surfaces. For the porous surface, an irradiation of 5 min was needed to completely negative the PCR signal. The Z value estimating the sensitivity of SARS-CoV-2 to UV-C in the experimental conditions of our cabinet was shown to be > 0.5820 m2/J. These results illustrate the ability of this apparatus to inactivate rapidly and definitively high loads of SARS-CoV-2 deposited on porous or non-porous supports and open new perspectives on material decontamination using UV-C.
Investigating the spatial and temporal variation of Aerosols and Cloud Parameters over South Asia, using remote sensing.
Authors: malik mahnoor , Tariq Salman , Haq Zia Ul 
Published: March 2023

Abstract
Aerosol optical depth (AOD) is the most crucial factor in determining how aerosol at local, and regional levels are spatiotemporally distributed. In this study, data has been obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard the Aqua satellite to examine spatiotemporal variations in aerosol optical depth (AOD) and their effects on the Angstrom Exponent (AE), and clouds parameters, namely cloud fraction (CF), cloud optical thickness (COT), cloud top pressure (CTP), cloud top temperature (CTT), ice cloud water path (ICWP), liquid cloud water path (LCWP), ice cloud effective radius (ICER), and liquid cloud effective radius (LCER) over South Asia from July 2002 to July 2021. The highest values of AOD (0.53–0.7) were observed in the Indo-Gangetic basin IGB region over South Asia. The highest values of AE (1.36 to 1.7) were observed in south-eastern India, Nepal, north-western Bangladesh, and Bhutan. The highest value of AOD is observed to be 0.7 in the IGB region during summer. The lowest value of AOD (0.2) was observed in winter. The highest mean value of AOD (0.63 ± 0.09) observed in Bangladesh is due to the noteworthy increase in agricultural activities. The negative correlation between AOD and AE was noticed in Karachi (-0.24), Lahore (-0.04), Rawalpindi (-0.08), Mumbai (-0.03), Kathmandu (-0.49), Colombo (-0.19), and in Kabul (-0.51). A positive correlation is observed in Delhi (0.21), Bangalore (0.09), and Dhaka (0.17).
Scalable Computer Interactive Teaching System Based on Large Scale Multimedia Data Analysis
Authors: Dong Zhang , Qi Zhu 
Published: March 2023

Abstract
The emergence of computer interactive teaching system greatly facilitates the needs of students to learn anytime and anywhere at this stage. At the same time, the increase of online course video, voice, files and other teaching resources also puts forward higher requirements for the operation of the teaching system. Based on the actual needs of online teaching, this paper designs an extensible computer interactive teaching system with the aid of large-scale multimedia data analysis algorithm. Because of the complexity and large volume of the data in the teaching system, this paper uses the linear data feature extraction algorithm of principal component analysis to extract the most basic low dimensional features of the data, and reconstructs the data through the linear transformation method to achieve data prediction. Most online course resources are displayed in the form of multimedia. Therefore, this paper uses robust component analysis to process multimedia data, especially for some problems such as data missing and multimedia data interference. This paper uses RC ranking related evaluation method and R-value evaluation method to evaluate the prediction data, which proves that parameter selection plays an important role in controlling sparse regression. And the actual teaching system resources related video and picture data were tested, and the results showed that the algorithm used in this paper had the best data recognition performance when the number of theme models was 100, which provided an important reference for the design of the system. Finally, this paper designs a computer interactive teaching system which includes three modules: administrator, student and teacher, and tests the system, which proves the practical operation possibility and application value of the system.
An adaptive matrix material extrusion optimization model for in situ impregnated continuous fiber reinforced 3D printing
Authors: Yang Jinxiu , Ma Yingxin , Chen Ling , Zhang Lijing , Wu Huanxiao , Yao Yuan 
Published: March 2023

Abstract
In order to improve the strength and surface quality of fiber-reinforced composite 3D printed parts and reduce the overfilling and underfilling caused by the manufacturing process of molten filaments under continuous paths, a co-extrusion control and optimization method based on matrix adaptive feeding with printing speed and deposition spacing is proposed in this paper. The method not only achieves surface void filling under different paths, but also reduces the porosity of the printed parts and improves the strength of the printed parts by adjusting the matrix extrusion under different deposition spacings. Compared with the common co-extruded model, the tensile strength is increased by 18% , the bending strength is increased by 23.4% and the porosity decreased by 54.4%. The proposed method is applicable to models with arbitrary continuous infill patterns and improves the quality of fiber-reinforced printed parts, especially to avoid structural failure due to uneven filling.
Genome-Wide identification and expression analysis of the polygalacturonase gene family in sweetpotato
Authors: He Peiwen , Zhang Jingzhen , Lv Zunfu , Cui Peng , Xu Ximing , George Melvin Sidikie , Lu Guoquan 
Published: March 2023

Abstract
Background: Polygalacturonase (PG), a crucial enzyme involved in pectin degradation, is associated with various plants' developmental and physiological processes such as seed germination, fruit ripening, fruit softening and plant organ abscission. However, the members of PG gene family in sweetpotato (Ipomoea batatas) have not been extensively identiﬁed.
Results:In this study, there were 103 PG genes identified in sweetpotato genome, which were phylogenetically clustered into divergent six clades. The gene structure characteristics of each clade were basically conserved. Subsequently, we renamed these PGs according to their locations of the chromosomes. The investigation of collinearity between the PGs in sweetpotato and other four species, contained Arabidopsis thaliana, Solanum lycopersicum, Malus domestica and Ziziphus jujuba, revealed important clues about the potential evolution of the PG family in sweetpotato. Gene duplication analysis showed that IbPGs with collinearity relationships were all derived from segmental duplications, and these genes were under purifying selection. In addition, each promoter regionof IbPG proteins contained cis-acting elements related to plant growth and development processes, environmental stress responses and hormone responses. Furthermore, the 103 IbPGs were differentially expressed in various tissues (leaf, stem, proximal end, distal end, root body, root stock, initiative storage root and fiber root) and under different abiotic stresses (salt, drought, cold, SA, MeJa and ABA treatment). IbPG038 and IbPG039were down-regulated with salt, SA and MeJa treatment. According to the further investigation, we found that IbPG006, IbPG034 and IbPG099had different patterns under the drought and salt stress in fiber rootof sweetpotato, which provided insights into functional differences among these genes.
Conclusion: A total of 103 IbPGs were identified and classified into six clades from sweetpotato genome. The results of RNA-Seq and qRT-PCR suggested that IbPG006, IbPG034 and IbPG099 might play a significant role in tissue specificity as well as drought and salt stress responses, which showed valuable information for further functional characterization and application of the IbPGs.
A Three-Arm Randomized Controlled Trial Using Ecological Momentary Intervention, Community Health Workers, and Video Feedback at Family Meals to Improve Child Cardiovascular Health: The Family Matters Study Design
Authors: Berge Jerica M. , Trofholz Amanda C. , Aqeel Marah , Norderud Kristin , Tate Allan , Fertig Angela R. , Loth Katie , Mendenhall Tai , Neumark-Sztainer Dianne 
Published: March 2023

Abstract
Background: Numerous observational studies show associations between family meal frequency and markers of child cardiovascular health including healthful diet quality and lower weight status. Some studies also show the “quality” of family meals, including dietary quality of the food served and the interpersonal atmosphere during meals, is associated with markers of child cardiovascular health. Additionally, prior intervention research indicates that immediate feedback on health behaviors (e.g., ecological momentary intervention (EMI), video feedback) increases the likelihood of behavior change. However, limited studies have tested the combination of these components in a rigorous clinical trial. The main aim of this paper is to describe the Family Matters study design, data collection protocols, measures, intervention components, process evaluation, and analysis plan.
Methods/design: The Family Matters intervention utilizes state-of-the-art intervention methods including EMI, video feedback, and home visiting by Community Health Workers (CHWs) to examine whether increasing the quantity (i.e., frequency) and quality of family meals (i.e., diet quality, interpersonal atmosphere) improves child cardiovascular health. Family Matters is an individual randomized controlled trial that tests combinations of the above factors across three study Arms: (1) EMI; (2) EMI+Virtual Home Visiting with CHW+Video Feedback; and (3) EMI+Hybrid Home Visiting with CHW+Video Feedback. The intervention will be carried out across 6 months with children ages 5-10 (n=525) with increased risk for cardiovascular disease (i.e., BMI ≥75%ile) from low income and racially/ethnically diverse households and their families. Data collection will occur at baseline, post-intervention, and 6 months post-intervention. Primary outcomes include child weight, diet quality, and neck circumference.
Discussion: This study will be the first to our knowledge to use multiple innovative methods simultaneously including ecological momentary assessment, intervention, video feedback and home visiting with CHWs within the novel intervention context of family meals to evaluate which combination of intervention components are most effective in improving child cardiovascular health. The Family Matters intervention has high potential public health impact as it aims to change clinical practice by creating a new model of care for child cardiovascular health in primary care.
Trial Registration: This trial is registered in clinicaltrials.gov (Trial ID: NCT02669797). Date recorded 5/02/22.
Uncertainty Assessment of Future Climate Change Using Bias-Corrected High-Resolution Multi-Regional Climate Model Datasets over East Asia
Authors: Park Changyong , Shin Seok-Woo , Juzbašić Ana , Cha Dong-Hyun , Choi Youngeun , Min Seung-Ki , Kim Yeon-Hee , Chang Eun-Chul , Suh Myoung-Seok , Ahn Joong-Bae , Byun Young-Hwa 
Published: March 2023

Abstract
The quantitative assessment of the uncertainty components of future climate projections is critical for decision-makers and organizations to establish climate change adaptation and mitigation strategies at regional or local scales. This is the first study in which the changes in the uncertainty components of future temperature and precipitation projections are quantitatively evaluated using multiple regional climate models over East Asia, vulnerable to future climate change. For temperature, internal variability and model uncertainty were the main factors affecting the near-term projections. The scenario uncertainty continued to increase and was estimated to be the dominant factor affecting the uncertainty after the mid-term projections. Although precipitation has the same main uncertainty factors as the temperature in the near-term projections, it significantly differs from temperature because the internal variability notably contributes to the fraction to the total variance, even in the long-term projections. The internal variability of the temperature and precipitation in the near-term projections was predicted to be larger in Korea than that in East Asia. This was confirmed by regional climate models as well as previous studies using global climate models as to the importance of internal variability at smaller regional scales during the near-term projections. This study is of significance because it provides new possibilities with respect to the consideration of climate uncertainties to the establishment of climate change policies in more detail on the regional scale.
Characterization of immune checkpoints expression and lymphocyte densities of Iranian breast cancer patients; the co-expression status and clinicopathological associates
Authors: Pournabee Mohammadhossein , Keshavarz-Fathi Mahsa , Esmaeili Pooyesh , Sharif Pouya Mahdavi , Nili Fatemeh , Jahanbin Behnaz 
Published: March 2023

Abstract
Background
Breast malignancies are now the most common and deadliest type of neoplasms among women worldwide. Novel therapeutic approaches are needed to combat advanced stages of breast cancer. In this study, we aimed to investigate the expression and co-expression status of three immune checkpoints (PD-1, PD-L1, and LAG-3), as well as tumor-infiltrating lymphocytes (TIL) scores, and to further establish their potential correlations with clinicopathologic features.
Methods
We performed a retrospective study on 361 pathologic samples of breast cancer. Immunohistochemistry was performed to assess the status of the immune checkpoint markers, and H&E staining was used to score TILs. The correlations of the immune checkpoint markers of tumor cells and tumor-associated immune cells and TIL scores with clinicopathological characteristics were analyzed.
Results
Out of 361 assessed samples, LAG-3 was positive in 51%, while IC PD-L1 and TC PD-L1 were detectable in 36% and 8.9%, respectively. Moreover, both IC PD-L1 and LAG-3 stained positively in 24.4% of samples. IC PD-L1 expression was significantly higher in tumors with higher nuclear, mitotic, and overall grades and tubule formation. In addition, TC PD-L1 and LAG-3 exhibited a similar trend for higher overall grading. Tumors with positive estrogen- and progesterone-receptor (ER and PR) expression had significantly lower IC PD-L1 and TC PD-L1 staining, while LAG-3 positivity was more prevalent in HER2 positive samples. Tumors that were positive for these biomarkers had significantly higher Ki-67 scores. LAG-3 expression showed significant correlations with PD-1 and IC PD-L1 expression. Besides, the co-expression of LAG-3 and IC PD-L1 was significantly more encountered in luminal B and triple-negative subtypes, compared to the luminal A subtype. Regarding TILs, their scoring was significantly higher in ER and PR negative and HER2 positive samples. Intriguingly, samples with positive staining for LAG-3, IC PD-L1, and TC PD-L1 had significantly higher TIL scorings.
Conclusions
Immune checkpoints show differentially different levels of expression in certain molecular subtypes of breast cancer. Moreover, they reveal a meaningful correlation with each other, proliferation indices, and histologic grades. Finally, a sizable proportion of breast cancers co-express PD-L1 and LAG-3, which will make them appropriate targets for future combined ICIs.
Quantifiable impact: monitoring landscape restoration from space
Authors: Vliet Mendy van der , de Jeu Richard , Malbeteau Yoann , Ghent Darren , Veal Karen , de Haas Sander , Zaan Thijs van der , Sinha Rajiv , Dash Saroj , Houborg Rasmus 
Published: March 2023

Abstract
Ecosystem conservation and restoration activities are critical to reduce the vulnerability of biodiversity to climate change. Although these activities need to be evaluated objectively and systematically, they are rarely analyzed from a global, multidimensional and multivariable perspective. Here we present a methodology to quantify the environmental impact of landscape restoration using long-term and high-resolution satellite observations. For two restoration areas in Tanzania, we find an increase in the amount of water retained by the soil (~0.01 m³ m⁻³, ~13% average increase), a soil temperature drop (~-0.5 °C) and an increase in surface greenness (~50% average increase) in 3,5 years. These datasets illuminate the impact of restoration initiatives on the landscape across their lifecycle, and support the reporting of comprehensive metrics to donors and partners. High quality satellite observations from commercial providers and space agencies are now achieving frequency, resolution, and accuracy that can effectively evaluate restoration activities.
Knowledge and practice of the physical activity prescription by generalists and specialist physicians of the City of Kinshasa: A cross-sectional study
Authors: Bowamou Christian Khalifa Emeka , Aliocha Nkodila Natuhoyila , Ekisawa Constant Nkiama , Jean-Paul Divengi Nzambi , Eleuthère Kintoki Vita , Benjamin Longo-Mbenza , Jean-Réné M’buyamba-kabangu , Bernard Kianu phanzu 
Published: March 2023

Abstract
Background
The health benefits of regular physical activity (PA) are well documented. However, several people in both developed and developing countries do not meet PA recommendations. Health professionals are believed to be potential PA promoters. The purpose of this study is to gain insight into general and specialist practitioners' knowledge, practices and PA prescription-related factors in private and public hospitals in Kinshasa.
Methods
A multicenter cross-sectional analytical study was conducted among general and specialist practitioners in the Democratic Republic of the Congo's capital using a declarative and anonymous questionnaire.
Results
Overall, 40.2% of the participants were interested in their patient’s PA, 2.3% prescribed PA, and 0.9% did it correctly. Specialist physicians (SPs) prescribed PA more frequently than general practitioners (GP), and private hospital physicians prescribed PA more frequently than public hospital physicians. Five factors were independently associated with participants in prescribing PA: being in a private hospital increased the likelihood of prescribing PA by twofold (aOR, 1.83; 95% CI, 0.99–3.39; p = 0.055), being an SP increased the likelihood by sixfold (aOR, 6.22; 95% CI, 3.78–10.51; p = 0.000), being an internist increase the likelihood by sixfold (aOR, 5.81; 95% CI, 3.45–9.78; p = 0.000), being cardiologist by a factor of 12 (aOR, 12.91; 95% CI, 4.37–38.15; p = 0.000) and knowing the benefits of PA by a factor of 2 (aOR, 2.29; 95% CI, 1.29–4.08; p = 0.006). The most common reason given for a lack of interest in patients' PA, followed by a lack of knowledge about current PA prescribing recommendations and a lack of time.
Conclusions
SPs and professionals in the private health sector were the most interested in their patients’ PA. A small portion of them actually prescribed it, and only a tiny proportion did it correctly. This bleak picture highlights a need to rethink the undergraduate medical curricula, especially about teachings on the importance and use of PA as a medicine in its own right in disease prevention and treatment.