bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Effectiveness of Non-pharmaceutical Interventions on Influenza During the Coronavirus Disease-2019 Pandemic by Time-series Forecasting
Authors: Kim Hyun Kyung , Min Kyung-Duk , Cho Sung-il 
Published: June 2023

Abstract
Background
Coronavirus disease 2019 (COVID-19) was first identified in South Korea during the 2019–2020 seasonal influenza epidemic. The social distancing measures, as effective non-pharmaceutical interventions (NPIs), adopted to mitigate the spread of COVID-19 might have influenced influenza activity. We evaluated IFV(influenza virus) activity during the COVID-19 pandemic and the effect of NPI intensity on influenza transmission.
Methods
IFV activity and epidemic duration during COVID-19 pandemic were predicted under a counterfactual scenario with no NPIs against COVID-19. The Seasonal Autoregressive Integrated Moving Average Model was used to quantify the effects of NPIs on the transmission of influenza virus. Influenza-like illness/1000 outpatients and IFV positivity rate from the 2011–2012 to 2021–2022 seasons were used in this study.
Results
Comparison of the 2020–2021 and 2021–2022 seasonal influenza activities with those in 2013–2019 showed that COVID-19 outbreaks and associated NPIs such as face mask use, school closures, and travel restrictions reduced the influenza incidence by 91%. Without NPIs against COVID-19, the rates of influenza-like illness and influenza virus positivity would have been high during the influenza epidemic season, as in previous seasons. NPI intensity decreased the transmission of influenza; the magnitude of the reduction increased as the intensity of social-distancing measures increased (step-by-step daily recovery, 58.10%; special quarantine measures, 95.12%).
Conclusions
Our results suggest that NPIs and personal hygiene can be used to suppress influenza transmission. NPIs against COVID-19 may be useful strategies for the prevention and control of influenza epidemics.
Collateral Status Combined with Preprocedural SII predicts the risk of futile recanalization in acute BAO patients: a randomized controlled trial
Authors: Liu Yao-Wu , Muhammad Bilal , Yuan Qi-Yang , Li Shuo , Yang Jin-Jin , Du Bo , Cheng Yan-Bo , Mu Ying-Feng , Zhu Shi-Guang , Geng De-qin 
Published: June 2023

Abstract
Background:Endovascular therapy (EVT) that might improve the outcome of patients with acute basilar artery occlusion remains controversial. The objective of this study was to investigate functional outcomes at 3 months after endovascular therapy in patients with acute basilar artery occlusion (ABAO) and to predict the futile recanalization.
Methods:The clinical data of acute basilar artery occlusion patients treated with endovascular therapy was collected from January 2019 to October 2022. Using the angiographic collateral grading system for basilar artery occlusion (ACGS-BAO) to evaluate collateral status. Futile recanalization was deﬁned as an mRS score of 3-6 at 3 months. Association of ACGS-BAO and the preprocedural SII with futile recanalization was analyzed using logistic regression models.
Results:The analysis of acute basilar artery occlusion patients showed that 47(64.38%) developed futile recanalization and 23(31.5%) died of 73 patients. Multivariate logistic analysis showed that ACGS-BAO (OR= 0.281, 95% CI = 0.132-0.600) and ln(SII) (OR= 2.482, 95% CI = 1.308-4.707) were independently associated with futile recanalization. In receiver operating characteristic analysis, the area under the curve for ACGS-BAO and SII were 0.737 and 0.703 (95% CI= 0.617-0.857, P＝0.001 and 95% CI=0.582-0.824, P=0.001), respectively. The effects of ACGS-BAO and ln(SII) on futile recanalization were similar in all subgroups (P> 0.10 for all interactions).
Conclusion: Our study suggested that bad collateral status and high inflammatory levels are independent predictors of futile recanalization after endovascular treatment in patients with ABAO.
Genetic diversity of SARS-CoV-2 in Southern Vietnam in 2020-2021
Authors: Gladkikh Anna S. , Cao Thang M. , Klyuchnikova Ekaterina O. , Dao Manh H. , Sharova Alena A. , Melnichenko Vasilina D. , Popova Margarita R. , Arbuzova Tatiana V. , Sbarzaglia Valeriya A. , Tsyganova Nadezhda A. , Ramsay Edward , Dedkov Vladimir G. 
Published: June 2023

Abstract
Background
Since its beginnings in 2019, the COVID-19 pandemic is still a problem of global medical concern. Southern Vietnam is one of the country's vast regions, including 20 provinces and the densely populated metropolis Ho Chi Minh City. The study aimed to investigate the epidemiology of COVID-19, including whole-genome sequencing of SARS-CoV-2 samples from Southern Vietnam, during the period January 2020 to December 2021 for identification of the main circulating variants.
Methods
Epidemiological data were obtained from the Department of Preventive Medicine of the Vietnamese Ministry of Health. To identify circulating variants, RNA, extracted from 126 nasopharyngeal swabs of patients with suspected COVID-19 were sequenced on Illunina MiSeq to obtain near complete genomes SARS-CoV-2.
Results
Due to the effectiveness of restrictive measures in Vietnam, it was possible to keep incidence at a low level. The partial relaxation of restrictive measures, and the spread of Delta lineages, contributed to the beginning of a logarithmic increase in incidence. Lineages 20A-H circulated in Southern Vietnam during 2020. Spread of the Delta lineage in Southern Vietnam began in March 2021, causing a logarithmic rise in the number of COVID-19 cases.
Conclusions
Pandemic dynamics in Vietnam feature specific variations in incidence, and these reflect the success of the restrictive measures put in place during the early stages of the pandemic.
Left with a Sisyphean task – the experiences of nurse practitioners with treating non-suicidal self-injury in the emergency department: a descriptive qualitative study
Authors: Roed Kickan , Brauner Cecilie Rostrup , Yigzaw Senayt , Midtgaard Julie 
Published: June 2023

Abstract
Background
Non-suicidal self-injury (NSSI) is a prevalent phenomenon in somatic emergency departments, where nurses are the most consistent group of healthcare professionals who treat people with NSSI, which means they may affect the NSSI trajectory and help-seeking in the future. The objective of this study was to describe the experiences of nurse practitioners with treatment of people presenting with NSSI in the emergency department.
Methods
Individual, semi-structured telephone interviews were conducted with seventeen purposefully recruited nurse practitioners from three emergency departments in the Capital Region of Denmark. Interview transcripts were analysed using inductive content analysis, as described by Graneheim and Lundman.
Results
The manifest content analysis resulted in the formulation of three categories and 10 subcategories indicating that nurse practitioners feel confident and competent in treating physical injuries due to NSSI but insecure about how to provide adequate care and engage in conversations about NSSI and mental wellbeing with people with NSSI. The underlying meaning of the content was interpreted and formulated as the latent theme 'Left with a Sisyphean task’, which reflects the nurses’ feeling of being handed the responsibility for performing a laborious, never-ending, and futile task.
Conclusion
Providing nurse practitioners with training and guidelines specifically tailored to NSSI to direct their decision making and enhance their confidence in their interactions with people with NSSI appears warranted.
Are maternal wards in government hospitals disaster prepared?: Systematic review
Authors: Horn Carla , Bam Nokwanda E. , Matsipane Molekodi 
Published: June 2023

Abstract

Background
Disasters go hand in hand with injuries and medical emergencies. Hospitals need to be able to function and render services during a disaster or pandemic outbreak and overall disaster preparedness should be in place as disasters can strike at any moment. Disasters may include floods, earthquakes, and fires but also violence infliction and pandemics. Effective disaster preparedness would entail each hospital and every ward having its own individual plan in place and a prepared team to execute the plan. The purpose of this literature review was to explore the disaster-preparedness of maternity wards of a government hospital.
Methods
The research methodology consisted of a literature review of fewer than twenty articles and an analysis of the literature and aspects of disaster preparedness. All relevant databases were searched from 2005 to October 2022. The results were filtered by means of a PICO diagram and the inclusion and exclusion criteria. The researcher and co-reviewer reviewed the articles. The final list of articles were analysed and the key findings were extracted and analysed.
Findings:
An exploration of the literature may indicate which factors need to be improved. Findings indicate that many shortcomings can be identified as a repetitive pattern and theme that is evident in many of the articles reviewed. Key themes were identified such as special considerations for obstetric wards, guidelines for allocations of resources, surge capacity, communication systems, and a specialized train team for evacuation.
Conclusion
The implications of this study stretch from research knowledge and theory and implications for practice by making recommendations in both fields. The research findings may assist government hospitals and their obstetric wards to better understand and to improve their state of disaster preparedness.
Loss of Fascin2 increases susceptibility to cisplatin-induced hearing impairment and cochlear cell apoptosis in mice
Authors: Wang Yan , Liu Yingying , Xie Yi , Luan Jun , Liu Rongrong , Zhu Yongjia , Ma Ying , Fan Yi , Sun Yan , Shang Wenjing , Han Fengchan 
Published: June 2023

Abstract
Objectives. Deletion of Fscn2 gene in mice has been linked to progressive hearing loss and degeneration of cochlear cells. Cisplatin, an antitumor drug, can cause various side effects, including ototoxicity. The aim of this study was to investigate the effects of Fscn2 on cisplatin-induced hearing impairment in mice and to explore the possible mechanism.
Methods. Two-week-old Fscn2+/+ mice and Fscn2-/- mice were treated with two doses of cisplatin, with a 3-day recovery period in between. ABR (auditory evoked brain stem response) thresholds were measured and cochlear pathology was observed at 3 weeks of age.
Results. Both Fscn2+/+ and Fscn2-/- mice showed hearing loss under the effect of cisplatin, but the impairment was more severe in Fscn2-/- mice. Further experiments showed that the percentages of outer hair cell (OHC) and spiral ganglion neuron (SGN) loss were significantly higher in cisplatin-treated Fscn2-/- mice compared to Fscn2+/+ mice. Additionally, knockdown of Fscn2 in HEI-OC1 cells worsened cisplatin-induced cell apoptosis.
Conclusion. FSCN2 mediates reduction of CDDP induced ototoxicity by inhibiting cell apoptosis
Early Tongue Cancer Detection in Photographs Using a Pretrained Convolutional Neural Network
Authors: Lee Sung-Jae , Kwon Ik-Jae , Son Young-Don , Kim Jong-Hoon , Kwon Dohyun , Kim Bongju , Lee Jong-Ho , Kim Hang-Keun 
Published: June 2023

Abstract
Although the tongue is an easily visible organ, tongue cancer often goes undetected until an advanced stage because it is difficult to distinguish between malignant lesions and non-malignant lesions. This study assessed the detection potential of tongue cancer, precancerous lesions, and inflammatory lesions using a pretrained convolutional neural network (CNN) and evaluated its effectiveness in a situation with multiple classes and imbalanced datasets. In total, 1,810 tongue images, each carefully labeled by medical specialists, were used for model training. Transfer learning, data augmentation, and fine-tuning were used to overcome the problems associated with limited datasets. Also, the weight balancing method was introduced to mitigate class imbalance. Three popular pretrained CNNs, namely VGG16, Inception-ResNet-V2, and EfficientNet, were evaluated as a backbone network. The final model achieved an accuracy of 0.9167, a precision of 0.9212, a recall of 0.9167, and an F2 score of 0.9176. Our results show that a pretrained CNN with a moderate complexity and a deep architecture based on data bypassing can detect and differentiate tongue lesions by applying currently available deep learning techniques, even with multiple classes and a limited and disproportionate number of images. Thus, these strategies can facilitate timely tongue lesion diagnosis and prompt treatment.
Rh-Catalyzed cyclization of carbamates – synthesis of a new heterocyclic system: tetrahydro-3H-4-oxa-2-thia-2b-azacyclopropa[cd]pentalen-3-one 2,2-dioxide
Authors: Dil Kateryna V. , Kozyriev Yevhenii K. , Palchykov Vitalii A. 
Published: June 2023

Abstract
A simple method for the synthesis of five- and six-membered ring sulfone carbamates was elaborated. The method includes reaction of the corresponding starting alcohols with trichloroacetyl isocyanate following the hydrolysis of trichloroacetyl protecting group with potassium carbonate in methanol. Rh2(OAc)4 catalyzed cyclization of carbamates was investigated. It was shown that carbamates with saturated sulfone moiety tend to give products of elimination of carbamic acid (corresponding unsaturated sulfones) due to high acidity of C-H bond in α-position of sulfone ring. Unsaturated sulfone carbamate (1,1-dioxido-2,3-dihydrothiophen-3-yl carbamate) in similar reaction conditions afforded new heterocyclic system confirmed by 2D NMR: tetrahydro-3H-4-oxa-2-thia-2b-azacyclopropa[cd]pentalen-3-one 2,2-dioxide. This result can be explained by more favorable aziridination of the double C=C bond by the nitrenoid intermediate compared to the elimination pathway. In silico pharmacological profile of new heterocyclic compound was evaluated. The compound showed good ADME and acute toxicity properties and high probability level of anticancer activity for prostate carcinoma cell line PC-3.
Comparison of dye-based and probe-based RT-LAMP in detection of canine astrovirus
Authors: Shen Hai Xiao , Shen Haixiao , Yang Dequan , Li Xin , Ju Houbin , Ge Feifei , Yang Xianchao , Wang Jian , Xia Luming , Zhao Hongjin , Jiang Ping 
Published: June 2023

Abstract
A rapid and sensitive assay is essential to promote reliable surveillance and diagnosis of canine astrovirus (CaAstV). In this study, two real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays with high sensitivity, rapidity and reliability were developed using fluorescence dye and FRET-Based Assimilating Probes for real-time CaAstV detection. Two real time RT-LAMP assays specifically amplified the ORF2 gene of CaAstV and did not amplify other canine enterovirus nucleic acids. The limit of detection (LOD) of both the probe-based and dye-based RT-LAMPs was 100 copy/μL. Fluorescence signals were generated within 30 min for the lowest concentration of a standard RNA sample, which was significantly faster than that achieved by real-time fluorescence quantitative PCR (qRT-PCR) assay. In the clinical samples assay, the positive and negative agreement of the dye-based RT-LAMP assay with qRT-PCR was 87.5% (14/16) and 93.55% (29/31), respectively. The positive and negative agreement of the probe-based RT-LAMP assay with qRT-PCR was 94.11% (16/17) and 96.55% (28/29), respectively. The RT-LAMP assays developed in this study showed a high potential for use as an on-site diagnostic assay for rapid, specific and reliable detection of CaAstV in clinical samples.
Registration and Follow-up Study of Focused Ultrasound Ablation Surgery Treatment of Uterine Fibroids
Authors: Wang Ting , Wang Zhibiao , Wen Yingang 
Published: June 2023

Abstract
The prevalence of uterine fibroids is increasing year by year. Focused ultrasound ablation surgery (FUAS) has been widely used in the treatment of uterine fibroids due to its advantages of non-invasive, non-ionizing radiation and uterine preservation. In the treatment of uterine fibroids with FUAS, ultrasound images are used as the monitor of the surgical process. The combination of ultrasound image guidance and magnetic resonance image can achieve more accurate and efficient navigation in FUAS. The current image navigation is not accurate and intelligent. Therefore, this paper investigates intelligent registration of ultrasound images and magnetic resonance images and intelligent tracking and registration correction techniques for FUAS. In our study, 90% of the cases had good tracking effect, which was equal to the manual registration results of experienced doctors. 5% of cases need a small amount of manual adjustment. In addition, 5% of the cases could not achieve the expected tracking effect due to the large deformation of the body state during the treatment compared with the MRI examination.
Exploring Autophagy-Associated Genes in the Endometrium of Recurrent Spontaneous Abortion and Examining their Relationship with Immune Infiltration
Authors: Shuai Ruzhen , Li Dandan , Liu Dan , He Jing , Wu Qiong , Zhou Yuan 
Published: June 2023

Abstract
Recurrent spontaneous abortion (RSA) is a common complication in pregnant women. Autophagy impacts the initiation and progression of various diseases,the specific role of autophagy in the process of endometrial decidualization in RSA paitents is still largely unknown. The purpose of this research was to examine the involvement of autophagy-related genes (ARGs) in decidualization associated with RSA using bioinformatics approaches.To identify differentially expressed genes (DEGs), the Gene Expression Omnibus database provided gene expression datasets GSE165004 and GSE26787. ARGs were retrieved from the Human Autophagy Database and the Human Autophagy Modulator Database, and their intersection with DEGs resulted in 109 differentially expressed ARGs which were significantly enriched in 14 GO terms and and 10 KEGG pathways. To assess the diagnostic capacity of the genes, an receiver operating characteristic curve was utilized. Simultaneously,The disparities in RSA immune microenvironments between low- and high- expression gene groups were analyzed using CIBERSORT, indicating that AKT2, BCL2L1, CTNNB1, GRB2, GSK3B, PTEN, and PTPN11 may be linked to the immune response during decidualization within the endometrial microenvironment. Among them, hub genes exhibited positive correlations with neutrophils, mast cells, and dendritic cells, and negative correlations with plasma, memory B and naive B cells. The results show that the 10 DEARGs (AKT2, RPS27A, PTPN11, PIK3CD, PTEN, CTNNB1, KRAS, GSK3B, BCL2L1, and GRB2) could act as potential biomarkers for the diagnosis of RSA. Furthermore, a noteworthy correlation was detected between DEARGs and the immune landscape of the endometrium in RSA patients.
Exploration of Underlying Mechanisms for Modified Xi-Xin-TangIII in Treating Alzheimer’s Disease through Network Integration Investigation
Authors: Zhao Kun , Zhang Hui , Bei Shifang , Wu Yinyan , Zhao Dongliang , Lin Jianyang , Wang Xiangyang , Wang Yuanwei 
Published: June 2023

Abstract
Background
Chinese natural herbal formula modified Xi-Xin-Tang III (mXXTIII) exhibits therapeutic effects for Alzheimer’s disease(AD). This study aimed to explore the underlying mechanism of mXXTIII for treating AD and discover the main active ingredients and potential drug targets through a combination of network pharmacology-based strategy and molecular docking technique.
Methods
Single herbs in mXXTIII were screened for active ingredients, and target proteins were predicted. Target screening for AD was performed to establish a disease target database. Subsequently, a protein-protein interaction network was constructed and the correlation between proteins in the network was used to obtain gene clusters, export the subnetwork, and analyze the biological processes facilitated by the targets in this subnetwork. GO and KEGG enrichment analyses of key genes were conducted using the DAVID database. Finally, molecular docking of critical targets and active ingredients was conducted and their interaction patterns were visualized.
Results
The research received 81 active ingredients, 519 targets, and 3089 disease targets. A total of 264 potential targets of mXXTIII against AD were identified by drawing a Venn diagram and the top action pathways were recognized according to GO and KEGG enrichment analysis.
Conclusion
Multiple active ingredients, targets, and pathways may be involved in intrinsic molecular actions of mXXTIII in the recovery of AD. The major active ingredients (quercetin, baicalein, formononetin, etc.), critical targets, and key pathways could have played more important roles. The findings may provide a reference for further studies and assessments on the mechanism of resisting AD.
Characteristics of timely integration of palliative care into oncology hospital care for patients with incurable cancer: results of a Delphi Study
Authors: Heipon Carly S. , Brom Linda , Linden Yvette M. van der , Tange Dorien , Reyners Anna K. L. , Raijmakers Natasja J. H. 
Published: June 2023

Abstract

Purpose
To identify elements of timely integration of palliative care (PC) in oncology hospital care from best practices. Thereafter, to assess the level of consensus among oncology and PC specialists and patient- and relative representatives on the characteristics of timely integration of PC.
Methods
A three round modified Delphi study was conducted. The expert panel consisted of 83 healthcare professionals (HCPs) from 21 Dutch hospitals (43 physicians, 40 nurses), 6 patient- and 2 relative representatives. The first round regarded four elements of integrated PC: (1) identification of potential PC needs (2), advance care planning (ACP), (3) routine symptom monitoring and (4) involvement of the specialist palliative care team (SPCT). In subsequent rounds, the panellists assessed which characteristics were triggers for initiating an element. Consensus was set a priori at ≥ 70%.
Results
A total of 71 (78%) panellists completed the first questionnaire, 65 (71%) the second, and 49 (54%) the third. Panellists agreed that in all patients with incurable cancer PC needs should be assessed (97%), symptoms monitored (91%) and ACP initiated (86%). The SPCT should be involved at the patient’s request (86%) or when patients suffer from increased symptom burden on multiple dimensions (76%). Patients with a life expectancy of < 3 months should be offered a consultation (71%).
Conclusion
The expert panel agreed that timely integration of PC into oncology is important for all patients with incurable cancer, using early identification, ACP and routine symptom monitoring. Involvement of the SPCT is specifically needed in patients with multidimensional symptom burden and in those nearing death.