bluuebunny/crossref_metadata_2025_split huggingface dataset:
Odor-evoked transcriptomics ofAedes aegyptimosquitoes
Authors: Mappin Fredis , Bellantuono Anthony J. , Ebrahimi Babak , DeGennaro Matthew 
Published: March 2023

SummaryModulation of odorant receptors mRNA induced by prolonged odor exposure is highly correlated with ligand-receptor interactions inDrosophilaas well as mammals of the Muridae family. If this response feature is conserved in other organisms, this presents a potentially potent initial screening tool when searching for novel receptor-ligand interactions in species with predominantly orphan olfactory receptors. We demonstrate that mRNA modulation in response to 1-octen-3-ol odor exposure occurs in a time- and concentration-dependent manner inAedes aegyptimosquitoes. To investigate gene expression patterns at a global level, we generated an odor-evoked transcriptome associated with 1-octen-3-ol odor exposure. Transcriptomic data revealed that ORs and OBPs were transcriptionally responsive whereas other chemosensory gene families showed little to no differential expression. Alongside chemosensory gene expression changes, transcriptomic analysis found that prolonged exposure to 1-octen-3-ol modulated xenobiotic response genes, primarily members of the cytochrome P450, insect cuticle proteins, and glucuronosyltransferases families. Together, these findings suggest that mRNA transcriptional modulation caused by prolonged odor exposure is pervasive across taxa and accompanied by the activation of xenobiotic responses. Furthermore, odor-evoked transcriptomics create a potential screening tool for filtering and identification of chemosensory and xenobiotic targets of interest.
Crystal structure of mRNA cap (guanine-N7) methyltransferase E12 subunit from monkeypox virus and discovery of its inhibitors
Authors: Wang De-Ping , Zhao Rong , Wang Hao-Feng , Wang Mei-Yue , Hu Wen-Shu , Lin Meng-Meng , Shu Wen , Cao Ji-Min , Cui Wen , Zhou Xin 
Published: March 2023

AbstractIn July 2022, the World Health Organization announced monkeypox as a public health emergency of international concern (PHEIC), over 85,000 global cases have been reported currently. However, preventive and therapeutic treatments are very limited. The monkeypox virus (MPXV) E12, an mRNA capping enzyme small subunit, is essential for the methyltransferase activity of RNA capping enzymes of MPXV. Here, we solved a 2.16 Å crystal structure of E12. We also docked the D1 subunit c-terminal domain (D1CTD) of vaccinia virus (VACV) with E12 to analyze the critical residues of interface between them. These residues are used for drug screening. The top six compounds are Rutin, Quercitrin, Epigallocatechin, Rosuvastatin, 5-hydroxy-L-Tryptophan, and Deferasirox. These findings may provide insights into the development of anti-MPXV drugs.
Urine proteomic characterization of active and recovered COVID-19 patients
Authors: Sun Jianping , Wei Jing , Yu Haibin , Sun Haidan , Liu Xiaoyan , Zhang Yonghong , Shao Chen , Sun Wei , Zhang Jing , Gao Youhe 
Published: March 2023

AbstractBackgroundThe molecular changes in COVID-19 patients have been reported in many studies. However, there were limited attention has been given to the disease sequelae in the recovered COVID-19 patients.MethodsHere, we profiled the urine proteome of a cohort of 29 COVID-19 patients in their disease onset and recovery period, including mild, severe, and fatal patients and survivors who recovered from mild or severe symptoms.ResultsThe molecular changes in the COVID-19 onset period suggest that viral infections, immune response changes, multiple organ damage, cell injury, coagulation system changes and metabolic changes are associated with COVID-19 progression. The patients who recovered from COVID-19 still exhibited an innate immune response, coagulation system changes and central nervous system changes. We also proposed four potential biomarkers to monitor the whole progression period of COVID-19.ConclusionsOur findings provide valuable knowledge about the potential molecular pathological changes and biomarkers that can be used to monitor the whole period of COVID-19.
Antagonistic Neural Circuits Drive Opposing Behaviors towards the Young in Females
Authors: Mei Long , Yan Rongzhen , Yin Luping , Sullivan Regina , Lin Dayu 
Published: March 2023

AbstractIn many species, including mice, females show strikingly different pup-directed behaviors based on their reproductive state1,2. Naïve wild female mice often kill pups while lactating females are dedicated to pup caring3,4. The neural mechanisms that mediate infanticide and its switch to maternal behaviors during motherhood remain unclear. Here, based on the hypothesis that maternal and infanticidal behaviors are supported by distinct and competing neural circuits5,6, we used the medial preoptic area (MPOA), a key site for maternal behaviors7–11, as a starting point and identified three MPOA-connected brain regions that drive differential negative pup-directed behaviors. Further functional manipulation andin vivorecording revealed that estrogen receptor alpha (Esr1) expressing cells in the principal nucleus of the bed nucleus of stria terminalis (BNSTprEsr1) are necessary, sufficient, and naturally activated during infanticide in female mice. Furthermore, MPOAEsr1and BNSTprEsr1neurons form reciprocal inhibition and change their excitability in opposite directions with reproductive state. The shift in balance between BNSTprEsr1and MPOAEsr1cell activity is likely a key mechanism for the behavioral switch during motherhood.
Reconstituting spore cortex peptidoglycan biosynthesis reveals a deacetylase that catalyzes transamidation
Authors: Tobin Micaela J. , Cho Stephen Y. , Profy William , Ryan Tessa M. , Le Donna H. , Lin Crystal , Yip Elaine Z. , Dorsey Jack L. , Levy Blake R. , Rhodes Jillian D. , Welsh Michael A. 
Published: March 2023

ABSTRACTSome bacteria survive in nutrient-poor environments and resist killing by antimicrobials by forming spores. The cortex layer of the peptidoglycan cell wall that surrounds mature spores contains a unique modification, muramic-δ-lactam, that is essential for spore germination and outgrowth. Two proteins, the amidase CwlD and the deacetylase PdaA, are required for muramic-δ-lactam synthesis in cells, but their combined ability to generate muramic-δ-lactam has not been directly demonstrated. Here we report an in vitro reconstitution of cortex peptidoglycan biosynthesis, and we show that CwlD and PdaA together are sufficient for muramic-δ-lactam formation. Our method enables characterization of the individual reaction steps, and we show for the first time that PdaA has transamidase activity, catalyzing both the deacetylation ofN-acetylmuramic acid and cyclization of the product to form muramic-δ-lactam. This activity is unique among peptidoglycan deacetylases and is notable because it may involve the direct ligation of a carboxylic acid with a primary amine. Our reconstitution products are nearly identical to the cortex peptidoglycan found in spores, and we expect that they will be useful substrates for future studies of enzymes that act on the spore cortex.
Resolving the developmental mechanisms of coagulation abnormalities characteristic of SARS-CoV2 based on single-cell transcriptome analysis
Authors: Luo Xizi , Zhang Nan , Liu Yuntao , Du Beibei , Wang Xuan , Zhao Tianxu , Liu Bingqiang , Zhao Shishun , Qiu Jiazhang , Wang Guoqing 
Published: March 2023

AbstractThe COVID-19 outbreak caused by the SARS-CoV-2 virus has developed into a global health emergency. In addition to causing respiratory symptoms following SARS-CoV-2 infection, COVID-19-associated coagulopathy (CAC) is the main cause of death in patients with severe COVID-19. In this study, we performed single-cell sequencing analysis of the right ventricular free wall tissue from healthy donors, patients who died in the hypercoagulable phase of CAC, and patients in the fibrinolytic phase of CAC. Among these, we collected 61,187 cells, which were enriched in 24 immune cell subsets and 13 cardiac-resident cell subsets. We found that in response to SARS-CoV-2 infection, CD9highCCR2highmonocyte-derived mø promoted hyperactivation of the immune system and initiated the extrinsic coagulation pathway by activating CXCR-GNB/G-PI3K-AKT. This sequence of events is the main process contributing the development of coagulation disorders subsequent to SARS-CoV-2 infection. In the characteristic coagulation disorder caused by SARS-CoV-2, excessive immune activation is accompanied by an increase in cellular iron content, which in turn promotes oxidative stress and intensifies intercellular competition. This induces cells to alter their metabolic environment, resulting in an increase in sugar uptake, such as that via the glycosaminoglycan synthesis pathway, in CAC coagulation disorders. In addition, high levels of reactive oxygen species generated in response elevated iron levels promote the activation of unsaturated fatty acid metabolic pathways in endothelial cell subgroups, including vascular endothelial cells. This in turn promotes the excessive production of the toxic peroxidation by-product malondialdehyde, which exacerbates both the damage caused to endothelial cells and coagulation disorders.
Inhibition of SARS-CoV-2 3CLproin vitroby chemically modified tyrosinase from Agaricus bisporus
Authors: Aguilera David , Ortega-Alarcon David , Abian Olga , Velazquez-Campoy Adrian , Palomo Jose M. 
Published: March 2023

AbstractAntiviral compounds are crucial to controlling the SARS-CoV-2 pandemic. Approved drugs have been tested for their efficacy against COVID-19, and new pharmaceuticals are being developed as a complementary tool to vaccines However, there are not any effective treatment against this disease yet. In this work, a cheap and fast purification method of natural tyrosinase fromAgaricus bisporusfresh mushrooms was developed in order to evaluate the potential of this enzyme as a therapeutic protein by the inhibition of SARS-CoV-2 3CLpro protease activityin vitro. Tyrosinase showed a mild inhibition of 3CLpro of around 15%. Thus, different variants of this protein were synthesized through chemical modifications, covalently binding different tailor-made glycans and peptides to the amino terminal groups of the protein. These new tyrosinase conjugates were purified and characterized by circular dichroism and fluorescence spectroscopy analyses, and their stability under different conditions. Then all these tyrosinase conjugates were tested in 3CLpro protease inhibition. From them, the conjugate between tyrosinase and dextran-aspartic acid (6kDa) polymer showed the highest inhibition, with an IC50of 2.5 μg/ml and IC90of 5 μg/ml, results that highlight the potential use of modified tyrosinase as a therapeutic protein and opens the possibility of developing this and other enzymes as pharmaceutical drugs against diseases.
SARS-CoV-2 N-protein induces the formation of composite α-synuclein/N-protein fibrils that transform into a strain of α-synuclein fibrils
Authors: Semerdzhiev Slav A. , Segers-Nolten Ine , van der Schoot Paul , Blum Christian , Claessens Mireille M.A.E. 
Published: March 2023

AbstractThe presence of deposits of alpha-synuclein fibrils in cells of the brain are a hallmark of several α-synucleinopathies, including Parkinson’s disease. As most disease cases are not familial, it is likely that external factors play a role in disease onset. One of the external factors that may influence disease onset are viral infections. It has recently been shown that in the presence of SARS-Cov-2 N-protein, αS fibril formation is faster and proceeds in an unusual two-step aggregation process. Here, we show that faster fibril formation is not due to a SARS-CoV-2 N-protein-catalysed formation of an aggregation-prone nucleus. Instead, aggregation starts with the formation of a population of mixed αS/N-protein fibrils with low affinity for αS. After the depletion of N-protein, fibril formation comes to a halt, until a slow transformation to fibrils with characteristics of pure αS fibril strains occurs. This transformation into a strain of αS fibrils subsequently results in a second phase of fibril growth until a new equilibrium is reached. Our findings point at the possible relevance of fibril strain transformation in the cell-to-cell spread of the αS pathology and disease onset.
Prediction of the maintenance cost of general aviation aircraft based on engineering method
Authors: Chen Xiaonan , Chu Shiyong , Zhang Guanglin , Chen Xuanyou , Huang Jun , Yi Mingxu 
Published: March 2023

Purpose
General aviation aircraft has a wide range of applications, and effective cost management is one of the hot spots in the research of general aviation manufacturers. The purpose of this paper is to build a flexible engineering method to predict maintenance cost of general aviation aircraft.

Design/methodology/approach
To establish a reasonable general aviation aircraft maintenance cost prediction model, it is necessary to analyze the influencing factors and extract the main components of maintenance cost. The maintenance cost is divided by engineering method, and the estimation model of each component cost is established. Then, the general aviation aircraft maintenance cost model is obtained. The results show that the relative error of this method is between 13% and 20%, which has a good estimation accuracy and can be effectively used to estimate the maintenance cost of general aviation aircraft.

Findings
The maintenance cost plays an important role in the life cycle cost of general aviation aircraft. Accurate cost prediction method is of great significance to the optimal design of general aviation aircraft. However, there are few prediction models suitable for maintenance cost, the proposed approach is meaningful and quite desirable.

Originality/value
To some extent, this method overcomes the shortage of the work on maintenance cost prediction for general aviation aircraft. The model established in this paper has certain generality, which can provide some reference for general aviation aircraft design and operation enterprises.
Defying tradition or maintaining the status quo? Moving towards a new hybrid architecture studio education to support blended learning post-COVID-19
Authors: Zairul Mohd , Azli Mohd , Azlan Aznida 
Published: March 2023

PurposeThis study aims to provide insight into the patterns and trends in the literature on the direction of future studio education in architecture schools, and can be useful to architecture schools, researchers and boards of studies in making decisions on how to move forward post-COVID-19.Design/methodology/approachThis study conducted a thematic review (TR) from SCOPUS and WoS that discuss the direction of future architecture studio education in the post-COVID-19 era and their recommendations for how to move forward.FindingsThe final theme is feedback studies, innovation in studio teaching, and a new model for post-COVID-19 studio education.Research limitations/implicationsDue to the temporal constraints of the data investigated between 2020 and 2022 based on the post-COVID period, only 13 publications were assessed based on the search strings utilized in this study, considering the decision and efforts made after COVID-19 among the participating architectural schools.Practical implicationsThis study will prove helpful to architecture schools, studio education researchers and the architecture board of studies.Social implicationsThis study intends to promote blended learning and enables the student to access the materials from anywhere at any time while enjoying the benefits of face-to-face (F2F) support and instruction.Originality/valueThis is the first review paper for architecture studio education post-COVID-19, as well as the first to use a TR approach to analyze the available literature on the subject. The focus on the post-COVID-19 period and the use of TR allow for a comprehensive understanding of the trends and patterns in the literature on the direction of future studio education in architecture schools. Additionally, this research aligns with the United Nations Sustainable Development Goal 4 on ensuring inclusive and equitable quality education and promoting lifelong learning opportunities for all, as it seeks to address the challenges and opportunities presented by the pandemic to support the learning and development of architecture students.
Do repeated alliances within patent pools encourage enterprise innovation? Evidence from MPEG LA
Authors: Lai Liubin , Zhang Yunsheng 
Published: March 2023

Purpose
The purpose of this study is to investigate whether repeated alliances between two members of a patent pool boost enterprise innovation. Furthermore, this paper intends to determine whether the innovation performance becomes higher or lower based on the partnership characteristics.

Design/methodology/approach
In this empirical study, hierarchical regression is used to analyze the longitudinal data obtained from 12 patent pools managed by MPEG LA during a time period ranging from 2006 to 2018. The members of patent pools comprise research institutions, firms and universities. Research analyses are performed based on a sample of 68,400 member pairs who had established repeated alliances. The information regarding such pairs is gathered from public databases.

Findings
Repeated alliances positively correlate with enterprise innovation performance in patent pools; this performance is higher when the two enterprises have exploratory collaborations. Conversely, the performance is lower when the partners have a similar technology base and are engaged in a technological competition (competitive learning and patent litigation). Moreover, the performance is lower when one partner demonstrates higher network centrality and richer structural holes than the other partner.

Originality/value
Patent pools play an instrumental role in eliminating patent-licensing barriers, thereby allowing mutual acquisition of complementary technologies, and cooperatively strengthening technology development. From the perspective of theories of coopetition, knowledge management and social network, this study explores the impact of patent pools on enterprise innovation performance and ascertains the moderating roles of technology coopetition, technology similarity and network position, thereby expanding the scope of innovation effect in the context of patent pools.
Applying a new approach to the governance of healthcare quality at board level
Authors: Martin Jennifer , Khurshid Zuneera , Moore Gemma , Carton Michael , Fitzsimons John J. , Henry Colm , Flynn Maureen A. 
Published: March 2023

PurposeThis paper describes a quality improvement project to improve oversight of quality at national board level using statistical process control (SPC) methods, complimented by a qualitative experience of patients and frontline staff. It demonstrates the application of the “Picture-Understanding-Action” approach and shares the lessons learnt.Design/methodology/approachUsing co-design and applying the “Picture-Understanding-Action” approach, the project team supported the directors of the Irish health system to identify and test a qualitative and quantitative picture of the quality of care across the health system. A “Quality Profile” consisting of quantitative indicators, analysed using SPC methods was used to provide an overview of the “critical few” indicators across health and social care. Patient and front-line staff experiences added depth and context to the data. These methods were tested and evolved over the course of six meetings, leading to quality of care being prioritised and interrogated at board level.FindingsThis project resulted in the integration of quality as a substantive and prioritised agenda item. Using best practice SPC methods with associated training produced better understanding of performance of the system. In addition, bringing patient and staff experiences of quality to the forefront “people-ised” the data.Originality/valueThe application of the “Picture-Understanding-Action” approach facilitated the development of a co-designed quality agenda item. This is a novel process that shifted the focus from “providing” information to co-designing fit-for-purpose information at board level.
Humanitarian supply chains and innovation: a focus on US food banks
Authors: Shaheen Iana , Azadegan Arash , Davis Donna 
Published: March 2023

PurposeTo effectively meet their social objectives, humanitarian organizations need to be more innovative and find novel ways to stay competitive. Yet there has been limited focus on innovation by humanitarian organizations. Part of the issue is the lack of new practices and novel approaches that can be used as benchmarks. This study focuses on food banks, a critical hub for the delivery of food in humanitarian supply chains and where the use of innovation seems to be more reported on.Design/methodology/approachFocusing on resource scarcity, a commonly referenced constraint by humanitarian organizations, the authors study how food and fund scarcity (versus abundance) influence the innovation efforts of twelve food banks in the United States. This study observes variations in behavior before and during the coronavirus disease 2019 (COVID-19) pandemic.FindingsThe authors find that food banks operating in high resource scarcity (food-scarce and fund-scarce) settings focus on process innovations. Food banks operating in low resource scarcity (food-abundant and fund-abundant) settings focus on product innovations. Food banks operating in food-abundant and fund-scarce settings focus on marketing innovations. Food banks operating in food-scarce and fund-abundant settings show the most extensive focus on innovation by relying on imitative innovations. The innovation focus for most food banks switches to process innovation during the COVID pandemic.Originality/valueThe study breaks down resource scarcity specific to food banks by differentiating food and funds, a novel approach to studying scarcity. Findings are novel as they suggest that operating context has a highly differentiating effect on what food banks focus on in terms of innovation. Operating context can lead to focus on process, product, imitative of market-related innovations. Finally, the study is novel because it explores how change in the environmental context due to disruptions can drastically modify the innovation focus of food banks.
A balanced walking-clamp mechanism for inspection robot of transmission line
Authors: Chao Caixia , Mei Xin , Wei Yongle , Fang Lijin 
Published: March 2023

Purpose
This paper aims to design a walking-clamp mechanism for the inspection robot of transmission line. The focus for this design is on climbing ability and obstacle-crossing ability with a goal to create a novel walking-clamp mechanism that can clamp not only the line but also the obstacle.

Design/methodology/approach
A novel clamping jaw used in the walking-clamp mechanism is proposed. The clamping wheel is mounted on the lower end of clamping jaw to reduce the friction between the clamping jaw and the line, and the top end of clamping jaw is designed as a hook structure to clamp the obstacle. The working principle and force states of the walking-clamp mechanism clamping the line and obstacle are analyzed, and the simulation and prototype experiments are carried out.

Findings
The experimental results show that this mechanism can clamp the obstacle steadily, and the clamping forces of the front and back pairs of clamping jaws are almost equal during robot walking along the catenary-shaped line. It is in agreement with the theoretical analysis, and it demonstrates that this mechanism can meet the working requirements of inspection robot.

Practical implications
This novel mechanism can be used for inspection robot of transmission line, and it is beneficial for robot to complete long-distance inspection works.

Social implications
It stands to reduce costs related to inspection and improve the inspection efficiency.

Originality/value
Innovative features include its structure, working principle and force states.