bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of protein hydrolysate preparation from split gill mushroom Schizophyllum commune
Authors:   , Wongaem Aunchalee 
Published: September 2023

The optimum conditions for hydrolysate production from split gill mushroom protein using the microbial protease, Alcalase were investigated. The study was conducted using central composite design and response surface methodology. Three independent factors: hydrolysis temperature (45, 50, and 55 °C); hydrolysis time (60, 120, and 180 min); and enzyme to substrate ratio (2%, 4%, and 6% w/v) were varied, while the pH was fixed at pH 8. These three factors had a significant effect on the DH and ABTS radical-scavenging activity (p < 0.05). The optimum conditions obtained from experiments were: enzyme to substrate ratio 2%; hydrolysis time 161.4 min; and temperature 55 °C. The coefficient of determination (R2) of total protein and the IC50 value for ABTS radical-scavenging activity were 0.972 and 0.205 µg/mL, respectively, and the DH value was 50.882%. The hydrolysate was fractionated by molecular weight (MW) cut-off membranes (10, 5, 3, and 0.65 kDa). The < 0.65 kDa fraction had the highest radical scavenging ability (IC50 = 0.026 ± 0.004 µg/mL). The MW < 0.65 kDa fraction was separated by reversed-phase HPLC to yield four subfractions (F1–5). The F4 subfraction showed the highest maximum ABTS radical-scavenging activity and was selected for further analysis by quadrupole-time-of-flight-electron spin induction–mass spectrometry-based. Five antioxidant peptides were identified. In addition, the MW< 0.65 kDa fraction protected against oxidation-induced DNA damage in pBR322, pKS, and pUC19 plasmids. Furthermore, the MW < 0.65 kDa fraction, exhibited cellular antioxidant activity in a human intestinal cancer cell line (HT-29), which was dependent on the peptide concentration. These results suggested that split gill mushroom protein hydrolysate is a good source of bioactive peptides and has potential as a natural antioxidant in food production and cosmetic products.
Mechanisms of ceftriaxone resistance in neisseria gonorrhoeae isolated from Thai patients
Authors:   , Kueakulpattana Naris 
Published: September 2023

N. gonorrhoeae is the causative agent of gonorrhea, which is one of the most important sexually transmitted disease. The multidrug resistance in N. gonorrhoeae has been increasingly reported worldwide. The objectives of this study were to investigate the prevalence and the mechanisms of ceftriaxone resistance in N. gonorrhoeae Thai isolates, and to investigate the synergistic activities of antibiotic combinations against N. gonorrhoeae isolates with reduced susceptibility or resistance to ceftriaxone. A total of 134 N. gonorrhoeae isolates were included in this study. The resistance rates of ciprofloxacin, tetracycline, penicillin G, gentamicin, azithromycin, ertapenem, fosfomycin, cefixime, and ceftriaxone were 90.30%, 82.09%, 73.13%, 59.70%, 13.43%, 1.49%, 0%, 0%, and 0%, respectively. The high prevalence of multidrug-resistant N. gonorrhoeae was found in 82.84%. Although all isolates were susceptible to ceftriaxone (MIC range of 0.001 to 0.125 mg/L with MIC50 and MIC90 of 0.002 and 0.015 mg/L, respectively.), two (1.49%) of them displayed reduced susceptibility to ceftriaxone. This is the first report of 2 N. gonorrhoeae isolates with reduced susceptibility to ceftriaxone, NG-083 (ST723) and NG-091 (new ST) in Thailand. Both isolates had mosaic PBP2 pattern XXXIV, L421P substitution in PBP1, an adenine deletion in the 13-bp inverted repeat sequence of the mtrR promoter region, a H105Y substitution in the MtrR repressor, and G120K and A121N substitutions in PorB porin. The result from NG-MAST showed the heterogeneous populations (29 STs) among 31 representative strains of N. gonorrhoeae . The activities of ceftriaxone plus azithromycin, fosfomycin, gentamicin, or ertapenem were evaluated by checkerboard method against the 2 isolates with reduced susceptibility to ceftriaxone. There was no synergistic activity of these antibiotic combinations. The best combination was ceftriaxone plus azithromycin, which showed the lowest FICIs of additive and was confirmed by time-kill method. The results showed that this combination had no synergistic activity but had bactericidal activity. This study showed high resistance rates of antibiotics previously used for treatment of gonorrhea, e.g., penicillin G, tetracycline, and ciprofloxacin. However, no isolates were resistant to ceftriaxone, suggesting that it is still active against N. gonorrhoeae Thai isolates. Mutations in, penA, mtrR promoter, mtrR, and porB genes were associated with reduced susceptibility to ceftriaxone. Therefore, antimicrobial resistance surveillance and rapid diagnosis are important to limit the emergence and spread of antibiotic-resistant N. gonorrhoeae isolates.
Characterization of PmSpӓtzle and SPIPm5 from black tiger shrimp Penaeus monodon
Authors:   , Boonrawd Sittichai 
Published: September 2023

Humoral response proteins in shrimp, antimicrobial proteins (AMPs) and proteinase inhibitors, are implicated in host defenses against the pathogen infection because their expression is changed in response to microbial infection. After the pathogen infection in shrimp, the Spätzle protein is synthesized and activated the pathogenic signaling via Toll pathway by its active protein for the synthesis of AMPs. In this study, three PmSpätzle isoforms, PmSpz1, 2 and 3 genes, were identified in Penaeus monodon. The PmSpz1 gene was chosen for studied in details. Its gene was expressed in all shrimp tissues tested and in response to WSSV infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as an active form awaiting to activate Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV challenge. The recombinant of active Spätzle activated the synthesis of AMP genes: crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5. It is indicating that the expression of all antimicrobial proteins but not penaeidin5 was expressed under the regulation of Toll pathway. For SPIPm5 protein, the Kazal-type serine proteinase inhibitor from P. monodon was also studied. Its transcripts were also expressed in all tissues tested and its gene expression was up-regulated by heat stress, WSSV and YHV. Injection of rSPIPm5 into shrimp to mimic heat stress condition had more or less no effect on the crustinPm1, penaeidin3, penaeidin5, Hsp70, SPIPm2 and SPIPm5. The effect of rSPIPm5 protein inhibited the hemolymph proPO activity. In survival experiments, the rSPIPm5 could also prolong the shrimp from pathogen infection, WSSV, YHV, Vibrio harveyi and Vibrio parahaemolyticus in shrimp. The increased endurance against the microbial infection was due to the inhibitory effects presumably activated by rSPIPm5 on viral replication and bacterial growth but not the expression of AMPs gene. The microbial inhibitory effects may be activated by rSPIPm5. Therefore, the SPIPm5 plays an important role in shrimp innate immunity against the viral and bacterial infection besides its function as a proteinase inhibitor.
Comparative study between
vitamin B 1-6-12 and vitamin B 12 for neurosensory recovery
after bilateral sagittal split ramus osteotomy
Authors:   , Trising Worawee 
Published: September 2023

A major complication from Bilateral Sagittal Split Ramus Osteotomy (BSSRO) is inferior alveolar nerve injured that manifest as neurosensory disturbance (NSD) on the lower lip, chin. Although the NSD is not a life-threatening problem but it impacts the patient's daily life. Vitamin B is commonly used to treat peripheral neuropathy but it is only few study for NSD from BSSRO. The aim of this study was to evaluate the effect of oral vitamin B1-6-12 and vitamin B12 on NSD after BSSRO. The study design was as a randomized, single blinded control trial of 75 patients who were NSD from BSSRO (n=25). The first group was vitamin B1-6-12 group that taking 1 tablet 3 times daily. The second group was vitamin B12 group that taking 1 tablet 3 times daily. The third group was the control group without taking vitamin B. Clinical Neurosensory Testing (CNT) was performed with static two-point discrimination, moving two-point discrimination, light touch and pinprick at preoperation, immediate, 1 week, 1 month, 3 months and 6 months and converted to Global Sensitivity Score (GSS). GSS was calculated to recovery proportion and was analysed with Friedman test and post hoc analysis with Wilcoxon signed rank test for intragroup comparison on the passing time and Kruskal Wallis H test and post hoc with Mann Whitney U test were used for intergroup comparison. Results showed significantly increasing recovery improvement in all groups on the passing time (p<0.05). Vitamin B1-6-12 orally were significantly highest recovery proportion when comparing with and control vitamin B12 at 6 months (p=0.038, p=0.033).
Preparation and characterization of starch films reinforced with citrate cellulose nanocrystals
Authors:   , Nakarat Prapaporn 
Published: September 2023

Citrate-modified bacterial cellulose nanocrystals (citrate CNCs) were prepared by a green one-pot dual acid (citric and hydrochloric acids) method and used them to reinforce three types of starch films; corn, wheat and rice starch films. The average length and width of citrate CNCs were 583 and 46 nm, respectively. The degree of citrate substitution on CNCs was 0.075 and its crystallinity index was closely to the original bacterial cellulose. The effect of various amount of citrate CNCs (0-20%wt of starch) on film property was study. Three starch films showed the same trend of examination results. The film thickness of most films was in a range of 0.20-0.24 mm and the increase of CNCs amount increase the film turbidity. The FE-SEM morphology revealed the good dispersion of citrate CNCs in the starch film matrix. The addition of citrate CNCs in all starches showed the continuing enhancement of crystallinity, Tdeg, and mechanical property. The tensile strength of starch/citrate CNCs films increased gradually while Young’s modulus increased dramatically. This indicated that the elasticity of films decreased if the amount of citrate CNCs increased. Among of three starches, citrate CNCs exhibited the highest influence on the mechanical property of wheat starch films.
Gene expression of Bcl2a1 และ Cxcl9 in soft tissue covering bone grafting materials
Authors:   , Taveedach Borwonluk 
Published: September 2023

This study was to assess the expression of Bcl2a1 and Cxcl9 genes in soft tissues covering bone grafted with demineralized freeze-dried bone allograft (DFDBA) and deproteinized bovine bone mineral (DBBM) in comparison with ones covering without grafting. The animals were randomly separated into three groups of treatment. Calvarial defect models were created on parietal bones. Bare defect models without bone graft as a control group. After one and three months, animals were sacrificed. Soft tissues covering the defected area were collected by punch technique. RNA was isolated and proceeded to Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Statistical analysis indicated that in comparison to control group at one month, DFDBA and DBBM upregulated Bcl2a1 gene in soft tissue covering bone graft at P < 0.05. Allograft also upregulated Cxcl9 gene (P < 0.05). However, the comparison among groups at three months and comparison within group between one and three months shown the expression of both genes but without significant difference (P > 0.05).
Effect Of Methyl Formate-Methyl Acetate Treatment On Flexural Strength Between Denture Base And Self-Cured Hard Reline Materials
Authors:   , Puangpetch Mongkol 
Published: September 2023

The purpose of this study was to evaluate the effect of methyl formate-methyl acetate (MF-MA) surface treatment on the flexural strength between denture base and hard reline materials. 180 heat-cured acrylic denture base (Meliodent®) specimens were prepared according to ISO 20795-1 (2013) and divided into 18 groups with various autopolymerizing hard reline materials. Group I-III: relined with Unifast Trad®, Group IV-VI: relined with Kooliner® Group VII-IX: relined with Tokuyama® Rebase II Fast (without adhesive, with hardener), Group X-XII: relined with Tokuyama® Rebase II Fast (with adhesive and hardener), Group XIII-XV: relined with Tokuyama® Rebase II Fast (without adhesive and hardener), Group XVI-XVIII: relined with Tokuyama® Rebase II Fast (with adhesive, without hardener). Group I, IV ,VII and XIII were untreated surface (control groups), Group II, V, VIII and XIV were surface treated with methyl methacrylate (MMA) for 180 s and Group III, VI, IX and XV were surface treated with MF-MA solution for 15 s. Group X and XVI were surface treated with the manufacturer adhesive, Group XI and XVII were surface treated with MMA 180 s and the manufacturer adhesive, Group XII and XVIII were surface treated with MF-MA 15 s and the manufacturer adhesive. The flexural strength was measured using a Universal Testing Machine. The data were analyzed using two-way ANOVA (group I-IX) and one-way ANOVA (group I-XVIII). If the significant differences in the groups were found, the mean flexural strengths of the groups were compared using Tukey’s test at a 95 % confidence level. For Tokuyama® Rebase II Fast, the data were analyzed using three-way ANOVA (Hardener, Manufacturing Adhesive, Surface treatment). The reline material type and surface treatments significantly affected on the flexural strength (p<0.05). For each reline material, the flexural strength of the MF-MA treated group was significantly higher compared with that of the MMA treated group and the MMA treated group had higher flexural strength than the untreated group (p<0.05). For Tokuyama® Rebase II Fast, the surface treatment and manufacturing adhesive affected on the flexural strength (p< 0.05), but the hardener did not affected on the flexural strength (p> 0.05). Groups of additional surface treatment (MMA, and MF-MA) after applied with the adhesive significantly increased the flexural strength compared with the groups with only using the manufacturing adhesive (p<0.05). For the same surface treatment, the flexural strength of Unifast Trad® was significantly higher compared with Kooliner® (p<0.05). The flexural strength of Kooliner® was higher than that of Tokuyama® Rebase II Fast (p<0.05). This study suggests the application of MF-MA solutions for 15 s before relining procedure can increase the flexural strength between denture base and hard reline materials.
An association between job stress, social support and insomnia among public health workers in Thailand.
Authors:   , Paitool Kanokrat 
Published: September 2023

Sleep disorder is the global burden public health problem nowadays. Sleep disorders link to several health problem including mental health, cardiovascular diseases, and unintentional injury. Insomnia is a type of sleep disorders which have sign of cannot sleep or difficult to fall asleep, stay asleep or woke up at night, or both them. The insomnia rather difficultly to define, however an individual’s report is used to identify its severity. Public health workers is an occupation dealing with distribution and determinants of health-related among population. Those people work with the high pressure including workload, working hours, conflict between different beliefs, lack of concern from community, organize support, and infectious which leading mental health risk factors.This analytical cross-sectional study aimed to 1) access prevalence of insomnia and 2) study an association between job stress, social support and insomnia among public health workers in Thailand. The quota sampling technique was used to select participants among doctors, nurse, public health scholar and others whom work under the Ministry of Public Health, Thailand. A self-reported questionnaire was sent out via mail. Thai Job Content Questionnaire (Thai-JCQ) was applied to identify work related factors. Insomnia Severity Index questionnaire(ISI) was performed to classify insomnia level. Amount 325 participants were returned, the response rate was 90.6%. Binary logistic regression was performed to analyse the associations. The result found that, majority of respondents were female (80%). Average age was 35 years old and 67.69% graduated Bachelor’s degree. Average reported working time per week was 46 hours/week. 40% of them reported having enough monthly income without saving. More than half (52.6%) of respondents reported clinical insomnia. For job stress and social support, the results among clinical insomnia showed that 27.49 % had high level of job stress, while 43.27% reported low level of social support. Insomnia was associated with education level (P-value=0.03) and sufficiency of income (P-value<0.01). Job pyschological demand level and supervisor support level were associated with insomnia at p-value< 0.001 and p-value=0.04. In multivariate analysis, workers who reports high pyschological job demand level was increased 1.99-fold odds of having insomnia comparing to the one who reported low pyschological job demand level [OR = 1.99; 95%CI 1.24 – 3.00]. In addition, those who reported high supervisor support level was decreased 0.64-fold odds of having insomnia comparing to the one who reported low supervisor support level [OR = 0.64; 95%CI 0.41 – 0.99].
Trends and factors associated with breastfeeding in Thailand : a secondary analysis of the multiple indicator cluster survey 2005-2016
Authors:   , Chen Yuanpei 
Published: September 2023

This cross-sectional study was based on secondary data of Thailand Multiple Indicator Cluster Survey (MICS round 3, 4 and 5) between 2005 and 2016 and aimed to identify trends and factors associated with breastfeeding in Thailand. Subjects were selected from the three MICS surveys and then pooled together to form final weighted samples of 9757 children under 2 years for studying early initiation of breastfeeding and 2568 children under 6 months for exclusive breastfeeding. Univariate and multivariate logistic regression analyses were applied to identify trends and factors. Results indicated that the overall breastfeeding rates were low in spite of an overall slight increase in early initiation of breastfeeding from 27.4% in 2005-2006 to 36.7% in 2015-2016 and a significant rise in exclusive breastfeeding from 6.3% to 27.1% during the decade in Thailand. Factors positively associated with early initiation of breastfeeding (p < 0.05) were north (adjusted OR = 1.530) and south regions (adjusted OR = 1.857), age of child (adjusted OR = 1.008), children ever born (adjusted OR = 1.175), average size of child at birth (adjusted OR = 1.180), and weight of child at birth (adjusted OR = 1.217), while negatively associated factors consisted of age of mother (adjusted OR = 0.973), fourth wealth index quintile (adjusted OR = 0.855), primary (adjusted OR = 0.568), secondary (adjusted OR = 0.706) and higher (adjusted OR = 0.504) education levels of mother, delivered in non-public medical sector (adjusted OR = 0.550), times of received antenatal care (adjusted OR = 0.984), and caesarean section (adjusted OR = 0.448). By contrast, factors found positively related to exclusive breastfeeding (p<0.05) included rural area (adjusted OR = 1.417), north (adjusted OR = 2.948) and northeast regions (adjusted OR = 1.551), age of mother (adjusted OR = 1.023), , female child (adjusted OR = 1.527), Richest wealth index quintile (adjusted OR = 1.675), mothers with secondary educational level (adjusted OR = 3.867), and children ever born (adjusted OR = 1.171), while negatively associated factors included age of child (adjusted OR = 0.637), delivered in non-public medical sector (adjusted OR = 0.621), weight of child at birth (adjusted OR = 0.518), times of received antenatal care (adjusted OR = 0.924), and caesarean section (adjusted OR = 0.551). In addition, the variable of area changed from significance in unadjusted to non-significance in adjusted analysis due to the influence of such variables as region, wealth index quintile, place of delivery, size of child at birth, and children ever born. Accordingly, the researcher recommends increasing exclusive breastfeeding practice in Thailand by considering associated factors.
Validation of LAMP-LFD Ehrlichia canis DNA sensor Kit
Authors:   , Jirapiti Pipat 
Published: September 2023

Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed to replace the special equipment requirement for PCRs. This work is aimed to validate LAMP-LFD against nested PCR and single PCR. The validation results showed that nPCR, LAMP-LFD and sPCR are equally in specificity test meanwhile, they have different degrees of sensitivity. nPCR is determined to be the most sensitive method. However, sensitivity of LAMP-LFD and sPCR when compare to nPCR are 78.57% (95% CI= 63.19%-89.70%) and 40.48% (95% CI 25.63% to 56.72%) respectively. The Cohen’s kappa coefficient revealed that nPCR and LAMP-LFD is in substantial agreement (0.785, 89.28% observed agreement) but not sPCR. The application of LAMP-LFD is hence capable of being an alternative method of molecular diagnostic test for CME and a point-of-veterinary care device.
Genome characterization of quinolone resistance associated genes of flavobacterium columnare isolated from columnaris diseased asian sea bass (lates calcarif)ER)
Authors:   , Chokmangmeepisarn Putita 
Published: September 2023

Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and NA were 16 and 64 μg/mL respectively. The complete genome analysis revealed that most resistant genes were responsible for efflux pump activity. Moreover, the mutations within quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC, and parE were detected. The novel mutations at position 370 in gyrB and 389 in parE were founded in all quinolone resistant (QR) isolates. Moreover, mutation at position 83 in gyrA was responsible for OA resistant mechanism in QR isolates whereas mutations within gyrB, and parE were play role in both NA and OA resistant. On the other hands, the QS isolates were carried double mutations in parC at position 88 and 183. Thus, these mutations may not play important role in resistant against quinolones.