bluuebunny/crossref_metadata_2025_split huggingface dataset:
H2A.X promotes endosperm-specific DNA methylation in Arabidopsis thaliana
Authors: Frost Jennifer M. , Lee Jaehoon , Hsieh Ping-Hung , Lin Samuel J. H. , Min Yunsook , Bauer Matthew , Runkel Anne M. , Cho Hyung-Taeg , Hsieh Tzung-Fu , Fischer Robert L. , Choi Yeonhee 
Published: June 2023

Abstract
Background
H2A.X is an H2A variant histone in eukaryotes, unique for its ability to respond to DNA damage, initiating the DNA repair pathway. H2A.X replacement within the histone octamer is mediated by the FAcilitates Chromatin Transactions (FACT) complex, a key chromatin remodeler. FACT is required for DEMETER (DME)-mediated DNA demethylation at certain loci in Arabidopsis thaliana female gametophytes during reproduction. Here, we sought to investigate whether H2A.X is involved in DME- and FACT-mediated DNA demethylation during reproduction.
Results
H2A.X is encoded by two genes in Arabidopsis genome, HTA3 and HTA5. We generated h2a.x double mutants, which displayed a normal growth profile, whereby flowering time, seed development, and root tip organization, S-phase progression and proliferation were all normal. However, h2a.x mutants were more sensitive to genotoxic stress, consistent with previous reports. H2A.X fused to Green Fluorescent Protein (GFP) under the H2A.X promoter was highly expressed especially in newly developing Arabidopsis tissues, including in male and female gametophytes, where DME is also expressed. We examined DNA methylation in h2a.x developing seeds and seedlings using whole genome bisulfite sequencing, and found that CG DNA methylation is decreased genome-wide in h2a.x mutant seeds. Hypomethylation was most striking in transposon bodies, and occurred on both parental alleles in the developing endosperm, but not the embryo or seedling. h2a.x-mediated hypomethylated sites overlapped DME targets, but also included other loci, predominately located in heterochromatic transposons and intergenic DNA.
Conclusions
Our genome-wide methylation analyses suggest that H2A.X could function in preventing access of the DME demethylase to non-canonical sites. Alternatively, H2A.X may be involved in recruiting methyltransferases to those sites. Overall, our data suggest that H2A.X is required to maintain DNA methylation homeostasis in the unique chromatin environment of the Arabidopsis endosperm.
Incorporating Metaheuristic Optimization Algorithm ABO for Improvement of LEACH Protocol in Wireless Sensor Networks
Authors: Malik Monika , Josh Alok , Sakya Gayatri 
Published: June 2023

Abstract
The principal application of Wireless Sensor Network (WSN) is to perform sensing and tracking in the scenarios where continuous monitoring is required. At the same time tiny sensor node consumes energy in performing these tasks resulting in reduced performance of WSN due to signal loss and packet transmission. Many techniques were developed to overcome the issues of high energy consumption nodes but still have a problem identifying cluster heads since packet loss and signal transmission occurs during transmission. . This paper integrates a metaheuristic optimization algorithm to improve the performance of WSN. The metaheuristic African Buffalo-based Energy Optimized LEACH Protocol (MABEOLP) is designed to enhance communication performance in WSN. The proposed scheme recognize the less energy and high energy consumption nodes at an earlier stage of the energy-aware phase and the high energy consumption nodes are neglected .At the same time a temporary route is developed to avoid sudden signal failure and packet drop. The optimized model is implemented in Matlab ,it is tested for maximum of 125 nodes and later the performance parameters energy consumption, delay, throughput, packet transmission rate, and packet loss are compare with the existing methods.
Linum usitatissimum oil Fraction Reverse Cardiac Remodeling at Molecular level: Suppressing miRNA-29b and miRNA 1 genes in Isoproterenol In vivo Model
Authors: Boshra Sylvia A , Nazeam Jilan A. 
Published: June 2023

Abstract
Linum usitatissimum (flaxseed) produce one of the oldest commercial oils which use traditionally as a functional food for lowering cholesterol level. Nevertheless, to date, there is no scientific evidence to assess the role of flaxseed oil in cardiac remodeling management. The study aimed to clarifying the underlying mechanism of standardized oil to restore cardiac remodeling in a heart toxicity rat model induced by isoproterenol (ISO). Oil fraction was purified, and major components were identified by gas-chromatography-mass spectrometry (GC-MS). The in vivo tests were conducted by ISO (85 mg/kg/ twice subcutaneously) with 24 hours between each dose. The rats were treated with flaxseed oil fraction (100 mg/kg orally) and the same dose was used for omega 3 as a positive control group. GC- MS revealed that α-linolenic acid (24.6%), oleic acid (10.5%), 6-octadecenoic acid (Z), 2,3 dihydroxypropyl ester (9.0%), 2,3-dihydroxypropyl elaidate (7.0%), n-propyl 9,12,15-octadecatrienoate (6.0%) are the major components. After 4 weeks of oil uptake, the results revealed an improvement in cardiac function, a decrease in apoptosis, and simultaneous prevention of myocardial fibrosis. The levels of BNP, NT-pro-BNP, endothelin-1, Lp-PLA2, and MMP2, and cTnI and cTn were significantly decreased, and a higher plasma level of Topo 2B was observed, moreover, miRNA − 1 and 29b were downregulated. Current evidence provide insight into the mechanism of flaxseed oil to restore cardiac remodeling, which supports its future application as cardioprotective against heart diseases.
Using the Community Perception Tracker to inform COVID-19 response in Lebanon and Zimbabwe: A qualitative methods evaluation
Authors: Majorin Fiona , Jain Anika , Haddad Christine El , Zinyandu Eddington , Gharzeddine Ghassan , Chitando Mutsawashe , Maalouf Aline , Sithole Ntandoyenkosi , Doumit Rita , Azzalini Raissa , Heath Thomas , Seeley Janet , White Sian 
Published: June 2023

Abstract
Background
Despite the recognized importance of community engagement during disease outbreaks, methods describing how to operationalise engagement are lacking. The Community Perception Tracker (CPT) was designed by Oxfam to systematically record real-time information on disease perceptions and outbreak response actions in order to adapt programmes.
Methods
We conducted a phased, qualitative methods, process evaluation in Zimbabwe and Lebanon to understand whether the CPT approach was a feasible way to incorporate community perceptions into COVID-19 response programming and whether this resulted in more relevant programming. We conducted 3 rounds of interviews with 15 staff using the CPT, analysed programmatic data, and conducted multiple rounds of phone-based interviews with outbreak-affected populations (50 participants per country). Qualitative data were thematically analysed and quantitative data descriptively summarized.
Results
Initially CPT implementing staff struggled to differentiate how the CPT differed from other monitoring tools that they were familiar with and felt that the training did not convey the full process and its value. However, with practise, collaboration and iterative improvements to the recommended CPT steps, staff found the process to be feasible and a significant value-add to their programming. Staff initially focused more on quantitively summarizing perceptions but eventually developed processes for maximizing the qualitative data on perceptions too. Trends emerging from the CPT led to frequent programmatic tweaks to COVID-19 messaging and product distributions. Emergent trends in perceptions also led staff to work cross-sectorally and advocate to other actors on behalf of populations. Outbreak-affected populations exposed to the programmes reported high levels of knowledge about COVID-19 and reported they practiced preventative behaviours, although this waned with time. Most population members also felt the COVID-19 programmes were relevant to their needs and said that non-government organisations were a trusted source of information.
Conclusions
The CPT appears to be a promising approach for ensuring that community engagement is undertaken systematically and that community perspectives are actively incorporated to improve programming. While crisis-affected populations generally found the programmes to be useful and relevant and to have influenced their knowledge and behaviours, it is not possible to attribute this to the CPT approach due to the study design.
Maximizing Three Piece Penile Prosthesis Length: Our Technique and Outcomes
Authors: Celtik Kenan E. , Siegal Alexandra R. , Valenzuela Robert , Razdan Shirin 
Published: June 2023

Abstract
Three-piece inflatable penile prosthesis (IPP) is considered the gold standard surgical treatment for erectile dysfunction (ED). The most common cause of patient regret after penile prosthesis surgery is penile shortening. We sought to query a national database on average prosthesis length and describe our techniques to maximize cylinder sizes. We performed a retrospective review of the Coloplast database of 3-piece penile prosthesis cases performed over the span of 1 year nationally and by a single high volume surgeon and compared average cylinder lengths. Our review did not investigate whether adjunct tunical lengthening or girth enhancing procedures were performed. Between July 1, 2021 and June 27, 2022, 145 three piece penile prosthesis surgeries were performed at our institution. All were performed via a scrotal incision. Nationally, 32.8% were performed infrapubic while 58.1% were performed scrotal. The most prevalent cylinder length in our series was 22cm, compared to the most prevalent nationally of 20cm. Our technique to maximize cylinder length involves circumferential mobilization of the corporal bodies and corporal tissue sparing. Corporal tissue sparing is carried out by employing 9” Metzenbaum scissors rather than sequential dilators for corporal tunneling. The benefit of this approach is the ability to create a dorsal compartment to the level of the triangular ligament of the glans, thereby extending the space to the level of the midglans. This maneuver minimizes placement of the prosthesis anteriorly at the level of the fossa navicularis and prevents floppy glans that would otherwise compromise maximum cylinder length. Three piece penile prosthesis cylinder size can be maximized by circumferential mobilization of the corporal bodies, corporal sparing maneuvers, and the utilization of long Metzenbaum scissors for precise corporal tunneling.
Experimental Investigation on Demountable Steel-Concrete Composite Reduced Web Section (RWS) Connections Under Cyclic Loads
Authors: Almutairi Fahad Falah , Tsavdaridis Konstantinos Daniel , Alonso-Rodriguez Andres , Hajirasouliha Iman 
Published: June 2023

Abstract
This paper presents an experimental study on demountable steel-concrete composite reduced web section (RWS) connections for use in seismic areas. Four composite connection specimens were subjected to sagging and hogging moments to investigate the performance of RWS connections under reversible actions. For such purpose, a single opening was made near the beam-column joint. Focus was made on the effects of perforation location and composite beam-slab action induced by bolted shear studs within the protected zone. Results indicate that employing RWS connections for seismic retrofit is a viable solution capable of achieving a performance similar to that observed for RWS connections in new buildings. Notably, a Vierendeel mechanism is generated, hence allowing for plasticity development in the beam, All RWS connections were capable of achieving an interstory drift larger than 4%, thereby complying with the performance targets set by ANSI/AISC 358 − 16, ANSI/AISC 341 − 16 and EC8. Moreover, deformation of the stubs was slight enough to allow for dissasemblage after the tests, indicating that the proposed solution could enable for reuse and retrofit after earthquakes.
Host associated probiotics improve growth performance, biochemical composition, tissue histomorphology, and enhance protection against pathogenic bacteria in Malaysian mahseer, Tor tambroides juveniles
Authors: Hossain Mohammod Kamruzzaman , Ishak Sairatul Dahlianis , Iehata Shumpei , Noordin NoorDiyana Mat , Abduh Muhammad Yazed , Kader Md. Abdul , Ullah Moahmmad Asmat , Emilia Sharifah Noor , Sung Yeong Yik , Abol-Munafi Ambok Bolong 
Published: June 2023

Abstract
The origin of the probiotics, the age and size of the fish, the length, dosage, and technique of application, as well as the culture environment, affect the efficacy of probiotics. Juveniles of Tor tambroides (initial weight 2.53 ± 0.02 g) were used in the feeding experiment to compare the effects of host associated probiotics (HAPs) and commercially prepared probiotics (CP) on growth performances, biochemical composition, tissue histomorphology, and protection against infection. A total 360 fish were fed for 70 days where control fish received basal feed only, two HAPs (T1: Aeromonas sp. KT27; T2: Enterococcus sp. strain KM07) and a commercial probiotic (T3: CP, Bacillus sp.) received experimental diets (1×108 CFUg− 1). Fish from all treatments were exposed to the pathogen Vibrio parahaemolyticus at the completion of the feeding trial by intraperitoneal injection of the lethal dose (LD50: 1×108 CFUml− 1). The results revealed that fish fed diets supplemented with HAPs exceeded control fish in all growth metrics and had higher post-challenge test survival (%). The proximate composition of the whole body did not change within treatments. However, both HAPs (T1 and T2) treatments improved the levels of total n-6, C18:3n-3, and C18:2n-6 in T. tambroides' whole body. Based on histological analysis, both HAPs had a good impact on gut shape reflected by increased villus height, width, and area as well as better hypertrophic muscle growth. The dietary application of HAPs, notably Enterococcus sp. strain KM07, showed overall better performances in T. tambroides juveniles and may be used as a potential probiotic in T. tambroides aquaculture.
Facile synthesis of 5-aminopyrazolo-quinolones
Authors: Elbastawesy Mohammed A. I. , Aly Ashraf A , El-Shaier Yaseen A.M.M. , Brown Alan B. , Abuo-Rahma Gamal El-Din A. , Samir Mohamed , Ramadan Mohamed 
Published: June 2023

Abstract
Catalyzed by triethylamine (Et3N), 4-hydrazino-quinolin-2-one and 2-(1-ethoxy-substituted-ene)malononitrile were refluxed together in ethanol to give 5-amino-3-subsituted-1-(subsitituted-2-oxo-1,2-dihydroquinolin-4-yl)-1H-pyrazole-4-carbonitriles. The structures of the products were elucidated using 1H NMR, 13C NMR, and mass spectra along with elemental analyses. 2D NMR spectroscopy was also used to differentiate the assigned structures from other possible ring systems and regioisomers. A plausible mechanism for the formation of target compounds was discussed.
Effects of N application methods on cotton yield and fertilizer N recovery efficiency in salinity fields with drip irrigation under mulch film using 15N tracing technique
Authors: Luo Zhen , Tang Wei , Wang Xiaowen , Lu Hequan , Li Chenyang , Liang Jun , Kong Xiangqiang 
Published: June 2023

Abstract

Background
Drip irrigation under mulch film promotes a non-uniform salinity distribution in salt fields. To study the effect of different nitrogen (N) application methods on cotton plant growth, yield and fertilizer N recovery efficiency (FNRE) using drip irrigation under mulch film conditions, three N application methods were assigned: N applied under mulch film (low-salinity area; UM), N applied between mulch films (high-salinity area; BM), and half N applied under mulch film and half between mulch films (HUHB).
Results
Plant height, photosynthesis, Chl content, boll load, biomass, boll weight and boll density under UM were all significantly higher than those under the other two treatments. The N absorption of UM was higher than in the other two treatments, which might be attributed to the expression of GHNRT1.5 and GHNRT2.1. The net NO3− influx in the roots in UM increased significantly compared with that in BM. The yield and FNRE of UM were 3.9% and 9.1%, respectively, and were 26.52% and 90.36% higher than under HUHB and BM treatments.
Conclusions
UM not only improved cotton yield but also alleviated the pollution of N residue on drip irrigation under mulch film conditions in salt areas.
WITHDRAWN: Dung Beetle Optimization Algorithm with Multi-modal Deep Learning based Skin Cancer Classification on Dermoscopic Images
Authors: Ch Srilakshmi , Lydia Laxmi , N Ramakrishnaiah 
Published: June 2023

Abstract
Skin cancer (SC) is the most prevalent cancer globally. Clinical assessment of skin lesions is needed to assess the disease characteristics; but, it is varied in interpretation and limited by longer timelines. Dermoscopy refers to a non-invasive imaging method that permits dermatologists to examine skin lesions with improved visualization of surface and subsurface structures. Machine-learning (ML) and deep learning (DL) methods were established to support dermatologists and overcome the problem as accurate and earlier diagnoses of SC is critical to increasing survival rate of the patient. In recent years, SC classification on dermoscopy images using DL has received great deal of interest. DL algorithms, especially convolutional neural networks (CNNs), have proved promising outcomes in precisely classifying skin lesions and differentiating between benign and malignant cases. The study presents a new Dung Beetle Optimization Algorithm with Multi-modal Deep Learning based Skin Cancer Classification (DBOA-MMDLSCC) technique on dermoscopic images. The presented DBOA-MMDLSCC technique exploits the ensemble learning mechanism by the use of three DL models with metaheuristic based hyperparameter tuning. In the presented DBOA-MMDLSCC technique, U-Net++ model is utilized for skin lesion segmentation. For feature extraction, multi-modal DL model comprising three DL models namely Xception, Residual Network (ResNet), and SqueezeNet. Moreover, the hyperparameter tuning of the DL approaches takes place using the DBOA. Lastly, convolutional autoencoder (CAE) model is applied for detecting and classifying SC. A wide range of experiments were performed to exhibit the simulation results of the DBOA-MMDLSCC technique. The experimental values highlighted the improved performance of the DBOA-MMDLSCC technique in terms of different evaluation measures.
Comparison of Thromboembolic Complications After Carotid Artery Stenting with and without Using Protection Devices: A Meta-Analysis study Short title: Comparison of Thromboembolic Complications After Carotid Artery Stenting with and without Using Protection Devices
Authors: Sadr Maryam , Vakili Kimia , Hasani Fatemeh , Tavasol Arian , Zanjanbar Dorsa Bahrami , Madadi Reza , Keshavarzmotamed Atoosa , Anjomrooz Mehran , Rahmatian Aryoobarzan , Sayehmiri Fatemeh 
Published: June 2023

Abstract
Objective
This study has been designed to compare the rate of thromboembolic events during Carotid angioplasty and stenting (CAS) with and without embolic protection devices (EPDs). So we systematically reviewed the literature to find and select studies that compared the rate of embolic events during CAS with and without embolic protection devices and conducted a meta-analysis to find which way is better.
Methods
Embase, PubMed, and Web of Science databases were thoroughly searched. By using random-effects models, each estimation was executed. To assess the heterogeneity among the studies I2 index was used. Egger and Begg’s tests were applied to evaluate publication bias. Stata version 14.2 was used for the statistical analysis.
Results
For 25% of patients, EPD was used during CAS, and for 75%, did not. The prevalence of hypertension, diabetes mellitus, coronary artery disease, and cigarette smoking was 77%, 37%, 40%, and 44%. 52% of included patients were symptomatic, and 48% of them were asymptomatic .The mortality rate reduced from 2% in the no_EPD subgroup to 1% among the EPD subgroup. The occurrence of all other complications was also reportedly higher in patients who did not receive EPD, including major stroke and MI, except for minor events, which were reported to be almost the same in both subgroups.
Conclusion
we found out that the use of EPD can help with reducing the occurrence of thromboembolic complications of CAS, including MI, major stroke, and death. Altogether, our results suggest that the benefits of using EPD during CAS outweigh its risks.
Analysis of Advanced Deep Learning Approaches for the Multiple Bone Fracture detection
Authors: Lydia Laxmi , Kumar Kollati Vijaya , Rao Gudikandhula Narasimha 
Published: June 2023

Abstract
An immediate requirement in modern medicine is for computer-aided detection and diagnosis of bone fractures. Radiologists can save time and work more efficiently as a result. In the past, numerous different image processing methods were utilized to spot bone breaks. In the field of medical image processing, deep learning models in the form of specialized convolutional neural networks are currently in widespread usage. Its scope is broadened to include bone fracture detection in X-Ray images. The severity of injuries can be estimated by extracting information such as the presence of fractures, their locations, and the distances in length, width, and depth between the broken bones in an automated method. We present methods for identifying bone fractures in diagnostic imaging. The major objective is to use multiple deep learning models to detect these cracks. Patients' elbow, hand, and foot X-rays are separated into their own categories in the dataset. The proposed system analyzes or diagnoses the output using frame difference and a data analytics approach. All models have their feature extraction handled by the proposed method. Because it double-checks feature extractions, it rarely fails to detect a fracture. The proposed system is worn on the hand's wrist. This software tells users the state of their fractures once they answer a few simple questions and upload an X-ray. The image is subsequently processed by the application's built-in models, with all results (whether or not a fracture was identified) shown in the user interface. Python scripts are incorporated into the application's C#-based Dotnet framework. Better sensitivity and specificity are achieved, with an overall accuracy of 89%. Correct diagnosis is crucial since wrong diagnoses can have devastating effects on patients.
Effects of Different Profile Configurations on Soil Physical and Chemical Properties and Particle Fractal Dimension
Authors: Wang Na , Liu Zhe , Bian Deng peng , Lu Nan 
Published: June 2023

Abstract
An experimental area of different profile configurations was established in Meixian County, Baoji City, Shaanxi Province. In the experiment, loamy loess was selected as cultivable layer and two types of soil thickness of 40cm (plot1, plot3, plot5) and 60cm (plot2, plot4, plot6) were designed, in which the transition layer of plot1 and plot2 were fine sand, the transition layer of plot3 and plot4 were loamy loess, and the transition layer of plot5 and plot6 were clay. Results showed that the soil particles were mainly composed of silt loam and silt. Soil particles were basically distributed in the range of 2 ~ 50µm. The fractal dimension D of soil volume in each treatment was in the range of 2.229 ~ 2.484, and there was a significant positive correlation between soil clay particle and soil silt particle content (P < 0.01). Fractal dimension D of soil volume in the experimental area was significantly positively correlated with soil organic matter, total nitrogen and silt particle content (P < 0.01), and significantly negatively correlated with soil gravel particle content (P < 0.05); Therefore, it can be concluded that the soil texture can be better improved when the covering type is loamy loess, the soil thickness is 40cm and the transition layer is fine sand.