bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radiotheranostics in oncology: Making precision medicine possible
Authors: Aboagye Eric O. , Barwick Tara D. , Haberkorn Uwe 
Published: January 2023

AbstractA quintessential setting for precision medicine, theranostics refers to a rapidly evolving field of medicine in which disease is diagnosed followed by treatment of disease‐positive patients using tools for the therapy identical or similar to those used for the diagnosis. Against the backdrop of only‐treat‐when‐visualized, the goal is a high therapeutic index with efficacy markedly surpassing toxicity. Oncology leads the way in theranostics innovation, where the approach has become possible with the identification of unique proteins and other factors selectively expressed in cancer versus healthy tissue, advances in imaging technology able to report these tissue factors, and major understanding of targeting chemicals and nanodevices together with methods to attach labels or warheads for imaging and therapy. Radiotheranostics—using radiopharmaceuticals—is becoming routine in patients with prostate cancer and neuroendocrine tumors who express the proteins PSMA (prostate‐specific membrane antigen) and SSTR2 (somatostatin receptor 2), respectively, on their cancer. The palpable excitement in the field stems from the finding that a proportion of patients with large metastatic burden show complete and partial responses, and this outcome is catalyzing the search for more radiotheranostics approaches. Not every patient will benefit from radiotheranostics; but, for those who cross the target‐detected line, the likelihood of response is very high.
Kajian Kualitas Air dan Indeks Pencemaran Di Perairan Kampung Sejahtera Pulau Baai Kota Bengkulu
Authors: Putri Yulisah Dwi , Yuliza Elfi , Lizalidiawati Lizalidiawati 
Published: January 2023

Water quality is one of the important factors supporting the growth of mangrove ecosystems. The activites of Kampung Sejahtera’s people were allegedly polluted the waters and disturbing the mangrove ecosystem. This study aimed to identify the status of water quality and pollution index in the waters of Kampung Sejahtera, Bengkulu City. The method used is a direct measurement at the research location which was carried out at 9 location points, 5 points representing the waters near the settlements of the prosperous village residents, and 4 location points representing the waters near the mangrove ecosystem. Then the results are compared with the Decree of the State Minister of the Environment No. 51 of 2004 concerning seawater quality standards for marine biota and to determine the status of water quality by using the pollution index method based on the Decree of the State Minister of the Environment No. 115 of 2003. The results of this study indicate that the temperature parameter, salinity, and pH met the quality standard while the TSS and DO parameters did not fulfill the quality standard. Based on the results of the calculation of the pollution index that the waters of Kampung Sejahtera are in the category of good condition to lightly pollute.
Application of magnetic Method on Mapping the Geothermal Sources at Air Putih, Lebong, Bengkulu
Authors: Lestari Septi edang , Yunita Astra , Rahman Riskia Abdul , Refrizon  , Sugianto Nanang 
Published: January 2023

Measurements have been made using the geomagnetic method in the Air Putih Tourism Area, Tambang Sawah Village, Pinang Belayar District, Lebong Regency with an area of 2000 m x 2500. Data collection was carried out using a Proton Procession Magnetometer (PPM) model G-856, which is a device with a proton sensor. which works based on the precess of the proton nucleus due to changes in the surrounding magnetic field and is equipped with other technical equipment, such as portable GPS (Global positioning system), types of navigation (maps of survey areas), such as talkies, umbrellas, pens, and recorded data. can be observed.. This research serves to map the potenTial for geothermal (geothermal), because this method is one of the geophysical methods that has advanTages in finding geothermal sources.Data processing is carried out using only Microsoft excel which can only plot magnetic anomalies and interpret qualitatively into initial research. To get the total animali, correction of IGRF (International Geomagnetics Reference Field) and correction of Daily Magnetic Value (diurnal). The results of this measurement obtained a magnetic field anomaly graph. This graph shows the high magnetic susceptibility value is found at poinT 43 which is suspected to be an area of economic value rock minerals and is in fracture zones and rocks with low susceptibility values are found at point 29 which can be suspected as a source area. hot water (hydrothermal). Keywords :Geomagnetic, Anomaly, and Hydothermal
Pendugaan Air Tanah Menggunakan Metode Tahanan Jenis di Kelurahan Kandang Limun, Kota Bengkulu
Authors: Nuraini Fera , Siregar Ade suryani , Arum Dzirana Sekar , Suhendra  
Published: January 2023

Air tanah merupakan air yang menempati pori-pori suatu batuan di bawah permukaan tanah pada zona jenuh air. Air memiliki peranan penting bagi kebutuhan utama dalam kehidupan sehari-hari. Ketersediaan air juga berpengaruh pada tingkat lajunya pertumbuhan penduduk dan penggunaan air yang meningkat. Maka dilakukan penelitian tentang pendugaan potensi air tanah (akuifer) di Kelurahan Kadang Limun, Kota Bengkulu menggunakan metode Vertical Electrical Sounding (VES) Konfigurasi Sclumberger yang bertujuan untuk mengetahui ketersediaan air tanah. Data yang didapatkan kemudian diolah dengan menggunakan Software IPI2Win. Berdasarkan penelitian ini hasil yang didapatkan dari titik 1 dan 2 terdapat air tanah pada kedalaman berkisar antara 3,75-76,5 m dengan nilai resistivitas berkisar diantara 15,9-38,7 Ωm. Selain itu, dari titik 3 dan 4 lapisan air tanah (akuifer) terdapat di kedalaman berkisaran 3,32-35,54 m dengan nilai resistivitas 6,24-32,46 Ωm.
Teman Sebaya dan Persepsi Remaja Pedesaan tentang Pernikahan Dini terhadap Putus Sekolah akibat Kehamilan Pranikah
Authors: Jamir Andi Fatimah , Layuk Melda Sambo 
Published: January 2023

Masa pubertas seringkali merupakan masa dimana remaja melakukan perilaku seksual yang menimbulkan berbagai masalah. Perilaku seksual pranikah yang mengarah pada kehamilan dan pernikahan dini berkontribusi pada putus sekolah. Tujuan dari penelitian ini adalah untuk menguji pengaruh persepsi teman sebaya dan remaja desa tentang pernikahan dini terhadap putus sekolah dini akibat kehamilan pranikah. Jenis penelitian ini adalah penelitian analitik dengan desain studi kasus kontrol dan menggunakan pendekatan retrospektif. Populasi adalah remaja usia 15-17 tahun yang berada di wilayah kerja Puskesmas Rante Alang. Jumlah sampel 75 responden. Analisis data menggunakan uji statistik chi-square dengan tingkat hasil ρ < nilai α = 0,05. Hasil penelitian menunjukkan bahwa hasil uji statistik dengan menggunakan uji chi-square diperoleh nilai ρ = 0,001 < nilai α = 0,05 untuk pengaruh teman sebaya dan ρ = 0,000 < nilai α = 0,05 untuk pengaruh persepsi remaja mengenai pernikahan dini. Dapat disimpulkan bahwa ada pengaruh teman sebaya dan persepsi remaja mengenai pernikahan dini terhadap kasus putus sekolah akibat kehamilan pranikah di wilayah kerja Puskesmas Rante Alang Kabupaten Tana Toraja tahun 2021. Bagi petugas kesehatan perlu untuk lebih meningkatkan pelayanan kesehatan reproduksi pada remaja utamanya dalam memberikan penyuluhan atau konseling kesehatan reproduksi remaja. Bagi remaja, lebih pandai dalam memilah teman sebaya, dan perlu meningkatkan pengetahuan tentang dampak yang ditimbulkan oleh hamil di luar nikah atau pernikahan dini.
Identification of the adhesive domain of AtaA from Acinetobacter sp. Tol 5 and its application in immobilizing Escherichia coli
Authors: Yoshimoto Shogo , Aoki Sota , Ohara Yuki , Ishikawa Masahito , Suzuki Atsuo , Linke Dirk , Lupas Andrei N. , Hori Katsutoshi 
Published: January 2023

Cell immobilization is an important technique for efficiently utilizing whole-cell biocatalysts. We previously invented a method for bacterial cell immobilization using AtaA, a trimeric autotransporter adhesin from the highly sticky bacterium Acinetobacter sp. Tol 5. However, except for Acinetobacter species, only one bacterium has been successfully immobilized using AtaA. This is probably because the heterologous expression of large AtaA (1 MDa), that is a homotrimer of polypeptide chains composed of 3,630 amino acids, is difficult. In this study, we identified the adhesive domain of AtaA and constructed a miniaturized AtaA (mini-AtaA) to improve the heterologous expression of ataA. In-frame deletion mutants were used to perform functional mapping, revealing that the N-terminal head domain is essential for the adhesive feature of AtaA. The mini-AtaA, which contains a homotrimer of polypeptide chains from 775 amino acids and lacks the unnecessary part for its adhesion, was properly expressed in E. coli, and a larger amount of molecules was displayed on the cell surface than that of full-length AtaA (FL-AtaA). The immobilization ratio of E. coli cells expressing mini-AtaA on a polyurethane foam support was significantly higher compared to the cells with or without FL-AtaA expression, respectively. The expression of mini-AtaA in E. coli had little effect on the cell growth and the activity of another enzyme reflecting the production level, and the immobilized E. coli cells could be used for repetitive enzymatic reactions as a whole-cell catalyst
Development of the DNA-based voltammetric biosensor for detection of vincristine as anticancer drug
Authors: Abbasi Mahmoud , Alsaikhan Fahad , Obaid Rasha Fadhel , Jahani Shohreh , Biroudian Saeed , Oveisee Maziar , Arab Mohammad Reza , Aramesh-Boroujeni Zahra , Foroughi Mohammad Mehdi 
Published: January 2023

In the article presented herein, a deoxyribonucleic acid (DNA) biosensor is introduced for Vincristine determination in pharmaceutical preparations based on the modification of screen printed electrode (SPE) with double-stranded DNA (ds-DNA), polypyrrole (PP), peony-like CuO:Tb3+ nanostructure (P-L CuO:Tb3+ NS). The developed sensor indicated a wide linear response to Vincristine concentration ranged from 1.0 nM to 400.0 μM with a limit of detection as low as .21 nM. The intercalation of Vincristine with DNA guanine led to the response. The optimized parameters for the biosensor performance were ds-DNA/Vincristine interaction time, DNA concentration and type of buffer solution. The docking investigation confirm the minor groove interaction between guanine base at surface of or ds-DNA/PP/P-L CuO:Tb3+ NS/SPE and Vincristine. The proposed sensor could successfully determine Vincristine in Vincristine injections and biological fluids, with acceptable obtains.
In situ preparation of a Bi2O2CO3/BiOI with 2D/2D p-n heterojunction photocatalyst for water purification under visible light
Authors: Wu Xiaoge , Qin Nan , Yan Lei , Ji Renlong , Wu Di , Hou Zhenhua , Peng Weihua , Hou Jianhua 
Published: January 2023

Introduction: Semiconductors have similar crystal structures and matched energy levels could form a coupled heterojunction at an interface between them which may allow response to visible light, achieving efficient decomposition of organic compounds.Methods: The Bi2O2CO3/BiOI (BOC/BOI) with 2D/2D p-n heterojunction was prepared by one-pot room-temperature strategy. The prepared materials were tested by various technologies, and the three-dimensional structure, light absorption properties, electrochemical properties and other information were obtained. Photocatalytic tests have also been carried out.Results and discussion: BOC/BOI heterojunction with oxygen vacancies showed much higher photocatalytic activity than pure BOC and BOI. For example, the preferred BOC/BOI-0.5 heterojunction of the degradation rate for Rhodamine B (RhB) is 97.6 % within 2 h, which is 15.8 and 2.2 times faster than that of BiOI and BOC. In addition, the removal rates of tetracycline, ciprofloxacin and bisphenol A by BOC/ BOI-0.5 were 92.4, 80.3 and 68.6%, respectively. The 2D/2D structures of BOC/BOI-0.5 with rich in oxygen vacancies combined p-n junction can effectively inhibit the photoinduced electron-hole pair recombination and increase the production of active free radicals. The O2- and h+ are the main reactants, giving the composite catalyst potential for degrading a variety of pollutants.
Functional characterization of a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant from Stenotrophomonas maltophilia
Authors: Shi Weina , Lu Junwan , Feng Chunlin , Gao Mengdi , Li Anqi , Liu Shuang , Zhang Lei , Zhang Xueya , Li Qiaoling , Lin Hailong , Lin Xi , Li Kewei , Zhang Hailin , Hu Yunliang , Wang Guangli , Bao Qiyu , Jiang Weiyan 
Published: January 2023

BackgroundThe intrinsic resistance mechanism plays an essential role in the bacterial resistance to a variety of the antimicrobials. The aim of this study is to find the chromosome-encoded novel antimicrobial resistance gene in the clinical isolate.MethodsThe function of the predicted resistance gene was verified by gene cloning and antibiotic susceptibility test. Recombinant protein expression and enzyme kinetic studies were performed to explore the in vivo activity of the enzyme. Expression of the resistance gene exposed to antimicrobial was determined by RT-qPCR. Whole genome sequencing and bioinformatic analysis were applied to analyze the genetic context of the resistance gene.ResultsThe novel aminoglycoside (AG) resistance genes designated aph(9)-Ic and aph(9)-Ic1 confer resistance to spectinomycin, and a recombinant strain harboring aph(9)-Ic (pMD19-T-aph(9)-Ic/DH5α) showed a significantly increased minimum inhibitory concentration (MIC) level against spectinomycin compared with the control strains (DH5α and pMD19-T/DH5α). The result of the kinetic analysis of APH(9)-Ic was consistent with the MIC result for the recombinant pMD19-T-aph(9)-Ic/DH5α, showing the efficient catalytic activity for spectinomycin [kcat/Km ratio = (5.58 ± 0.31) × 104 M−1·s−1]. Whole-genome sequencing demonstrated that the aph(9)-Ic gene was located on the chromosome with a relatively conserved genetic environment, and no mobile genetic element was found in its surrounding region. Among all the function-characterized resistance genes, APH(9)-Ic shares the highest amino acid sequence identity of 33.75% with APH(9)-Ia.ConclusionWe characterized a novel AG resistance gene aph(9)-Ic and its variant aph(9)-Ic1 that mediated spectinomycin resistance from S. maltophilia. The identification of the novel AG resistance genes will assist us in elucidating the complexity of resistance mechanisms in microbial populations.
Ecological influence by colonization of fluoride-resistant Streptococcus mutans in oral biofilm
Authors: Shen Yan , Yu Fangzheng , Qiu Lili , Gao Mengjia , Xu Puxin , Zhang Lingjun , Liao Xiangyan , Wang Min , Hu Xiangyu , Sun Yan , Pan Yihuai 
Published: January 2023

BackgroundDental caries is one of the oldest and most common infections in humans. Improved oral hygiene practices and the presence of fluoride in dentifrices and mouth rinses have greatly reduced the prevalence of dental caries. However, increased fluoride resistance in microbial communities is concerning. Here, we studied the effect of fluoride-resistant Streptococcus mutans (S. mutans) on oral microbial ecology and compare it with wild-type S. mutans in vitro.MethodsBiofilm was evaluated for its polysaccharide content, scanning electron microscopy (SEM) imaging, acid-producing ability, and related lactic dehydrogenase (LDH), arginine deiminase (ADS), and urease enzymatic activity determination. Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the S. mutans ratio within the biofilm. It was followed by 16S rRNA sequencing to define the oral microbial community.ResultsFluoride-resistant S. mutans produced increased polysaccharides in presence of NaF (P &lt; 0.05). The enzymatic activities related to both acid and base generation were less affected by the fluoride. In presence of 275 ppm NaF, the pH in the fluoride-resistant strain sample was lower than the wild type. We observed that with the biofilm development and accumulative fluoride concentration, the fluoride-resistant strain had positive relationships with other bacteria within the oral microbial community, which enhanced its colonization and survival. Compared to the wild type, fluoride-resistant strain significantly increased the diversity and difference of oral microbial community at the initial stage of biofilm formation (4 and 24 h) and at a low fluoride environment (0 and 275 ppm NaF) (P &lt; 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that fluoride-resistant strain enhanced the metabolic pathways and glucose transfer.ConclusionsFluoride-resistant S. mutans affected the microecological balance of oral biofilm and its cariogenic properties in vitro, indicating its negative impact on fluoride's caries prevention effect.
CT characteristics and clinical implications of acute type A aortic intramural hematoma
Authors: Yen Hsu-Ting , Wu Chia-Chen , Lee Yi-Wei , Lo Chien-Ming , Chen Yen-Yu 
Published: January 2023

ObjectivesComputed tomography (CT) has been increasingly used in the diagnosis of acute aortic syndrome, and a number of high-risk CT imaging features have been reported. We aimed to identify CT imaging findings suggesting high-risk for acute aortic syndrome by examining clinical outcomes of patients with acute type A aortic intramural hematoma (TAIMH).MethodsThis retrospective study analyzed the relationship of clinical patient characteristics and imaging features with mortality and aortic events in 63 patients receiving initial medical treatment for TAIMH. Multivariate regression analysis was used to determine the predictors of aortic events, and the Kaplan–Meier method was used to analyze survival and aortic events.ResultsDuring a median follow-up of 4.2 years, 25 patients experienced aortic events and 40% of these occurred within 7 days of admission. In total, 12 patients experienced aortic death and 12 patients underwent open aortic surgery or endovascular stenting for aortic disease. In multivariate regression analysis, penetrating atherosclerotic ulcers (PAUs) or ulcer-like projections (ULPs) (P = 0.04) and pericardial effusion (P = 0.03) were independent predictors of aortic events. In the Cox regression model, PAUs/ULPs (P = 0.04) and pericardial effusion (P = 0.04) were independently associated with lower aortic event-free survival.ConclusionIdentification of high-risk CT features is important for clinical decision-making during TAIMH treatment. Early and frequent CT imaging follow-up is required in patients receiving medical treatment. PAUs/ULP and pericardial effusion were the strongest predictors of adverse aortic events.
Triglyceride-glucose index and estimated 10-year risk of a first hard cardiovascular event
Authors: Qu Hua , Long Lin-zi , Chen Li , Wu Han-tao , Fu Chang-geng , Zhang Shan-shan 
Published: January 2023

BackgroundWhether Triglyceride-glucose (TyG) index is associated with 10-year risk of a first hard atherosclerotic cardiovascular disease (ASCVD) event in the United States remains unclear.MethodsIn this cross-sectional study, the participants, ranged from 40 to 79 years old, were from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2018. TyG index was the independent variable and 10-year risk of a first hard ASCVD was the dependent variable. The other variables, such as age, gender, race, body mass index (BMI), hypertension treatment states, smoking states and low-density lipoprotein cholesterol (LDL-C) et al. were considered as the potential confounding factors. Multivariate linear regression models and smooth curve fittings were used to evaluate the association between TyG index and 10-year risk of a first hard ASCVD event.ResultsA total of 2,142 participants were included in the analysis. The results showed that TyG index was associated with an increased 10-year risk of a first hard ASCVD event [β = 2.208, 95% (1.716, 2.700), P &lt; 0.00001]. The association had statistical significance in both men [β = 3.862 95% CI (3.274, 4.450), P &lt; 0.00001] and women [β = 1.067, 95% CI (0.286, 1.849), P = 0.00756)] according to subgroup analysis. Smooth curve fittings revealed that TyG index was linearly associated with 10-year risk of ASCVD in both male and female.ConclusionTriglyceride-glucose index was associated with an increased 10-year risk of a first hard ASCVD event in the United States, suggesting it is necessary to monitor and control an appropriate range of TyG index.
Short-wall paste continuous mining and continuous backfilling for controlling industrial square surface subsidence
Authors: Meng Zixuan , Dong Yue , Zhang Xinguo , Jiao Fangshu , Fan Yaqi , Thammavongsa Champa 
Published: January 2023

To facilitate coal mining under the industrial square in Western China, short-wall paste continuous mining and continuous backfilling (PCMCB) is proposed to release the overlaid resources and handle coal gangue. First, the concept of short-wall PCMCB is presented, and the principle of surface subsidence is analyzed. Second, the mechanical model of the branch roadway and strength of the backfill body are established via mechanical derivations. Third, the proportion of backfill materials is calculated. Finally, field applications are carried out in the Dingjiaqu coal mine. The results show the following: 1) Based on the simply supported beam model of the roof and strength design model of the backfill body, the reasonable width of the branch roadway was determined as 5 m and late strength of the backfill body should exceed 2.62 MPa in the Dingjiaqu coal mine. 2) According to the design strength of the backfill body and backfill material proportion, the reasonable ratio of cement: fly ash: gangue: water was determined as 1:1.82:4.17:1.78. 3) In accordance with the equivalent mining height theory, the critical filling rate of the goaf should be more than 90% to ensure that the deformation is within the first level of building damage. 4) The field application shows that subsidence could be effectively controlled by backfilling, the overall surface subsidence was very small, and the maximum subsidence value (77 mm) was far lower than the deformation extremes of the building protection requirements. This study provides practical guidance for the exploitation of overlaid resources under similar shallow burial conditions, which can help in the disposal of coal gangue with considerable social and economic benefits with popularization and application.