bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlations Among Perceived Symptoms and Interferences, Barriers to Symptom Management, and Comfort Care in Nurses Caring for Chemotherapy and Transarterial Chemoembolization Patients
Authors: Kim Myoung Soo , Yoo Seonghyun 
Published: March 2023

Background
Nursing care, encompassing the physical, psychospiritual, sociocultural, and environmental aspects of care, should ensure patients’ comfort in both chemotherapy and transarterial chemoembolization (TACE) treatment.

Objective
The aim of this study was to examine the canonical correlations between perceived symptoms and interferences, barriers to symptom management, and comfort care in nurses caring for chemotherapy and TACE patients.

Methods
This cross-sectional study surveyed 259 nurses caring for patients undergoing chemotherapy (n = 109) and TACE (n = 150). Fisher exact test, t tests, χ
2 tests, Pearson correlations, and canonical correlations were performed.

Results
In the chemotherapy nurse group, higher perceived symptoms (R values = 0.74), higher perceived interference (R values = 0.84), and higher barriers to pain management (R values = 0.61) were associated with higher physical (R values = 0.58) and psychological (R values = 0.88) comfort care. In the TACE nurse group, the higher the perceived symptoms and perceived interference, the lower the perceived barriers to pain management, and lower barriers to nausea/vomiting management were associated with higher physical, psychological, sociocultural, and environmental care.

Conclusions
Nurses caring for TACE patients reported lower perceived symptom interference and comfort care, including physical, psychological, and environmental aspects, than those caring for chemotherapy patients. In addition, there was a canonical correlation among perceived symptoms, symptom interferences, barriers to pain management, and comfort care, including physical and psychological care of nurses caring for chemotherapy and TACE patients.

Implication for practice
Nurses caring for TACE patients need to provide physical, psychological, and environmental comfort care for their patients. Oncology nurses caring for chemotherapy and TACE patients should coordinate treatment for co-occurring symptom clusters to enhance comfort care.
Spiritual Care Expectations Among Cancer and Noncancer Patients With Life-Threatening Illnesses
Authors: Kang Kyung-Ah , Kim Shin-Jeong 
Published: March 2023

Background
Spirituality is a core element in holistic nursing care. Therefore, it is necessary to understand the spiritual care expectations of cancer and noncancer patients with life-threatening illnesses.

Objective
The aim of this study was to identify the spiritual care expectations of vulnerable patients with life-threatening illnesses.

Interventions/Methods
This study uses both quantitative and qualitative approaches, and data were collected from 232 patients. For quantitative data, we used the Nurse Spiritual Therapeutics Scale (NSTS), which comprises 20 items. Qualitative data were collected using an open-ended question. Quantitative data were analyzed using descriptive statistics, independent t tests, 1-way analysis of variance, and item and factor analysis. Qualitative data were analyzed using content analysis.

Results
The mean score of spiritual care expectations ranged from 2.27 to 3.07. There was a significant difference in NSTS mean score between cancer and noncancer patients. In exploratory factor analysis, NSTS was extracted into 3 factors and items belonging to the 3 factors showed similarity between cancer and noncancer patients. Qualitative data using content analysis revealed the following 3 themes: “treat with respect,” “religious support,” and “comfort with presence.” The 3 factors corresponded with 3 themes: factor I versus “treat with respect,” factor II versus “religious ritual,” and factor III versus “comfort with presence.”

Conclusions
Spiritual care expectations of cancer and non-cancer patients with life-threatening illnesses were identified and the findings provide valuable data regarding the expectations of patients’ spiritual care.

Implications for Practice
Our findings emphasize integrating patient-reported outcomes with spiritual care to stimulate patient-centered care, thus promoting holistic palliative or end-of-life care.
Peer Supporters’ Experience of Supporting Cancer Patients
Authors: Que Wen-qian , Zhao Jing-yi , Tang Jue , Su Xiao-qin , Li Jia-mei , Gu Chen-hao , Guo Yu-jie 
Published: March 2023

Background
With the widespread use of peer support in the cancer field, more and more cancer survivors are becoming supporters. However, they may bear a huge psychological burden in the peer support project. There has been little effort to analyze supporters’ experiences from a meta-perspective.

Objective
The aims of this study were to review the literature on the experience of patients serving as peer supporters, integrate qualitative data to explore the experiences of supporters participating in peer support programs, and provide suggestions for future researchers.

Interventions/Methods
China Knowledge Network, Wanfang Database, China Biomedical Literature Database, PubMed, Cochrane Library, Embase, CINAHL, and PsycINFO were searched. Titles, abstracts, and full texts were screened. Included articles (n = 10) underwent data extraction, the Joanna Briggs Institute Critical Appraisal Tool for qualitative researches (2016) quality evaluation, and thematic synthesis.

Results
The literature ultimately included 10 studies from which 29 themes were distilled and grouped into 2 main categories: benefits and challenges of peer support for supporters.

Conclusions
Peer supporters will not only gain social support, growth, and recovery but also experience various challenges when providing peer support. Both supporters’ and patients’ experiences of participating in peer support programs deserve the attention of researchers. Researchers need to be rigorous in controlling the implementation of peer support programs to help supporters gain and overcome challenges.

Implications for Practice
Future researchers can use study findings to better develop peer support programs. More peer support projects are needed to explore a standardized peer support training guide.
SEPT9 Expression in Hepatic Nodules: An Immunohistochemical Study of Hepatocellular Neoplasm and Metastasis
Authors: Kmeid Michel , Park Young Nyun , Chung Taek , Pacheco Richard R. , Arslan Mustafa Erdem , Lee Hwajeong 
Published: March 2023

The methylated SEPT9 DNA (mSEPT9) in plasma is a US Food and Drug Administration (FDA)-approved screening biomarker in colorectal cancer and is emerging as a promising diagnostic and prognostic biomarker in hepatocellular carcinoma (HCC). We evaluated the SEPT9 protein expression by immunohistochemistry (IHC) in various hepatic tumors from 164 hepatectomies and explants. Cases diagnosed as HCC (n=68), hepatocellular adenoma (n=31), dysplastic nodule (n=24), and metastasis (n=41) were retrieved. SEPT9 stain was performed on representative tissue blocks showing tumor/liver interface. For HCC, archived IHC (SATB2, CK19, CDX2, CK20, and CDH17) slides were also reviewed. The findings were correlated with demographics, risk factors, tumor size, alpha fetoprotein levels at diagnosis, T stage and oncologic outcomes, with significance defined as P<0.05. Percentage of SEPT9 positivity differed significantly among hepatocellular adenoma (3%), dysplastic nodule (0%), HCC (32%), and metastasis (83%, P<0.001). Compared with patients with SEPT9– HCC, those with SEPT9+ HCC were older (70 vs. 63 y, P=0.01). The extent of SEPT9 staining correlated with age (r

s
=0.31, P=0.01), tumor grade (r

s
=0.30, P=0.01), and extent of SATB2 staining (r

s
=0.28, P=0.02). No associations were found between SEPT9 staining and tumor size, T stage, risk factors, CK19, CDX2, CK20, or CDH17 expression, alpha fetoprotein levels at diagnosis, METAVIR fibrosis stage, and oncologic outcome in the HCC cohort. SEPT9 is likely implicated in liver carcinogenesis in a HCC subset. Similar to mSEPT9 DNA measurement in liquid biopsies, SEPT9 staining by IHC may prove helpful as an adjunct diagnostic biomarker with potential prognostic ramifications.
Effects of Hippotherapy on Postural Control in Children With Cerebral Palsy: A Systematic Review
Authors: Peia Fernanda , Veiga Nathalie Caroline , Gomes Ana Paula , Santos Bruna Neves dos , Marques Nívia Maria Silva , Glória Igor Phillip dos Santos , Goulardins Juliana Barbosa 
Published: March 2023

Purpose:
To summarize and update the current knowledge on the effectiveness of hippotherapy on postural control in children with cerebral palsy.

Methods:
Using a systematic review methodology, the electronic databases PubMed, Virtual Health Library, PEDro, Scielo, Embase, and Web of Science were searched for eligible articles from 2011 up to September 2021. Quality assessment of eligible studies was performed using the PEDro scale.

Results:
There were 239 identified studies. Eight clinical trials were selected. The total sample consisted of 264 individuals, with 134 allocated to the experimental group (hippotherapy) and 130 individuals to the control group (conventional therapy). Most studies had moderate to high methodological quality.

Conclusions:
Hippotherapy can be an effective intervention to improve several aspects related to postural control, such as static balance (especially in the sitting posture), dynamic balance, and alignment (body posture) in children aged 3 to 16 years, particularly with spastic hemiplegia or diplegia.

What This Adds to the Evidence:
This review summarizes studies that explore potential effects of hippotherapy on postural control in children with cerebral palsy.
Early intravenous Beta-Blockade with esmolol in adults with isolated severe Traumatic Brain Injury (EBB-TBI): protocol for a phase 2a intervention design study
Authors: Thomas Matt , Hayes Kati , White Paul , Ramesh Aravind , Culliford Lucy , Ackland Gareth , Pickering Anthony 
Published: March 2023

AbstractTraumatic brain injury is a leading cause of death and disability worldwide. Interventions that mitigate secondary brain injury have the potential to improve outcomes for patients and reduce the impact on communities and society. Increased circulating catecholamines are associated with worse outcomes and there is supportive animal data and indications in human studies of benefit from beta-blockade after severe traumatic brain injury. Here we present the protocol for a dose-finding study using esmolol in adults commenced within 24 hours of severe traumatic brain injury. Esmolol has practical advantages and theoretical benefits as a neuroprotective agent in this setting, but these must be balanced against the known risk of secondary injury from hypotension. The aim of this study is to determine a dose schedule for esmolol, using the continual reassessment method, that combines a clinically significant reduction in heart rate as a surrogate for catecholamine drive with maintenance of cerebral perfusion pressure. The maximum tolerated dosing schedule for esmolol can then be tested for patient benefit in subsequent randomised controlled trials.
A Systematic Review and Meta Synthesis Protocol for Examining the Factors behind African Nurses’ & Midwives Migration
Authors: Osei Stephanopoulos Kofi Junior , Barfi Michael Owusu , Frimpong Sandra , Bosro Dorinda Dela , Fosu Christopher Asamoah , Owusu Daniel , Amoako-Mensah Deborah Ntriwaa , Barimah Sammy Kwantwi , Agbavor Jerry Kofi Esinu , Delanyo Bertha Awo Agbesi 
Published: March 2023

AbstractBackgroundThere has been an increased rise of African nurses and midwives migrating to high income countries despite varying policies and restrictions to promote retention. The need to comprehensively understand the factors influencing the exodus is critical for policy formulation at the global, national, and facility levels.AimTo provide a comprehensive and a systematic review of the factors influencing African nurses and midwives’ migration to high income countries and the factors influencing such migrations.MethodsA systematic review and meta-synthesis design guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Statement guidelines would be used. Databases including CINAHL, Pubmed, MEDLINE, and EbscoHost will be searched using a PICOS selection criteria framework. Two independent reviewers would be involved in data extraction and meta-synthesis. A third reviewer would provide an arbitrary judgement when disagreements persist after discussion. A meta-synthesis and thematic analysis would be utilised to identify emerging themes and reporting themes identified in the literature.Ethics and disseminationThe study does not require ethical approval. The findings would be published in peer-review journals and presented at conferences.PROSPERO registration number:CRD42023395013Strengths and limitationsThe study would shed a comprehensive light on factors influencing the migration of African trained nurses outside the continent. This is relevant in informing policies that promote retention.The review is conducted following the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement (PRISMA). The methodology is rigorous and meets the standards of the PRISMA guidelines.Studies published in any language other than English would be excluded.The period restriction set could also limit the number of studies found for a comprehensive review.
The impact of Short Tandem Repeats on grey matter brain imaging derived phenotypes in UK Biobank
Authors: Sproviero William , Ghose Upamanyu , Winchester Laura M , Fernandes Marco , Newby Danielle , Sproviero Daisy , Amin Najaf , Smets Bart , He Karen Y. , Khramtsova Ekaterina A. , Patel Parth , Sarver Brice A. J. , Howe Trevor , Black Mary Helen , van Duijn Cornelia , Nevado-Holgado Alejo 
Published: March 2023

AbstractWe performed a genome-wide association study of 143,067 highly polymorphic short tandem repeats (STRs) with MRI brain grey matter volumes (GMVs) on 10,702 UK Biobank (UKB) participants, including 8,751 in the discovery stage and 1,701 in the replication analysis. STRs’ repeat lengths were estimated from the UKB whole-genome sequencing data using Expansion Hunter software.A total of 262 STRs reached genome-wide significance in the analyses of the autosomal and sex chromosomes’ (P= 6.9 × 10−8) in association with MRI -GMVs. Replication in a second batch extraction in the UKB and linkage disequilibrium (LD) analyses confirmed 12 associations of five STRs with hippocampal, intra-calcarine cortex, and cerebellum volumes with no evidence of single nucleotide polymorphisms (SNP) in LD detected in the surrounding DNA regions. Our study highlights the importance of STR variants involved in the genetic architecture of grey matter volumes.
Dobutamine Stress Echocardiography in Low-Gradient Aortic Stenosis
Authors: Borg Mogensen Nils Sofus , Ali Mulham , Carter-Storch Rasmus , Annabi Mohamed-Salah , Grenier-Delaney Jasmine , Møller Jacob Eifer , Øvrehus Kristian Altern , Pellikka Patricia A , Pibarot Philippe , Clavel Marie-Annick , Dahl Jordi Sanchez 
Published: March 2023

AbstractBackgroundDobutamine stress echocardiography (DSE) is recommended by guidelines to distinguish between true-severe and pseudo-severe aortic stenosis (AS) in patients with low-gradients and left ventricular ejection fraction (LVEF) <50%. However, DSE has mostly been tested in the setting of LVEF<35% and determination of AS severity has mostly been based on outcome data and surgeon’s evaluation. The purpose of this study was to examine the diagnostic accuracy of guideline recommendations for DSE, in patents with low-gradient severe AS with a wide range of LVEF and to examine the interaction between the diagnostic accuracy of DSE and LVEF. Furthermore, we wanted to study the safety and feasibility of DSE in patients with LVEF>50%.MethodsPatients with aortic mean gradient <40 mmHg, AVA <1.0 cm2, and stroke volume index ≤35 mL/m2undergoing DSE and Cardiac Computer Tomography (C-CT) were identified from three prospectively collected patient cohorts, and stratified according to LVEF; LVEF <35%, LVEF 35-50% & LVEF >50%. Severe AS was defined as AVC score ≥2000 AU among men, and ≥1200 AU for women on C-CT.ResultsTwo hundred twenty-one patients were included in the study. Seventy-eight (35%) presented with LVEF <35%, 67 (30%) with LVEF 35-50%, and 76 (34%) with LVEF >50%. DSE was performed without adverse symptoms or significant arrhythmias in 215 (96%) patients and stroke volume increased uniformly with no significant differences between groups (p=0.28).Mean gradient and Vmaxduring DSE showed significantly diagnostic heterogeneity between LVEF groups, being most precise when LVEF <35% (both AUC=0.90), albeit with optimal thresholds of 30 mmHg & 377 cm/s, and a limited diagnostic yield in patients with LVEF≥35% (AUC=0.67 in LVEF 35-50% and AUC 0.65 in LVEF≥35%). Using guideline thresholds led to a sensitivity and specificity of 49%/84% for all patients with LVEF <50%.ConclusionWhile DSE is safe and leads to a uniform increase in stroke volume in patients with low gradient AS regardless of baseline LVEF, the association between DSE gradients and AS severity assessed by C-CT demonstrates important heterogeneity depending on LVEF, with highest accuracy in patients with LVEF <35%.Clinical perspectiveWhat is new?Dobutamine stress echocardiography (DSE) is safe in patients with low-gradient AS with LVEF >50%, and leads to similar increase in stroke volume as in patients with LVEF <50%.The diagnostic accuracy of DSE, compared to AVC as the reference for severe AS, depends on LVEF with highest accuracy in patients with LVEF <35%.Suggested reference thresholds for DSE may not be the most accurate for AS severity, when compared to AVC.What are the clinical implications?Based on our study, we suggest that DSE should primarily be used for determining AS severity in patients with LVEF <35%.
Long non-coding RNASNHG8drives stress granule formation in tauopathies
Authors: Bhagat Reshma , Minaya Miguel A. , Renganathan Arun , Mehra Muneshwar , Marsh Jacob , Martinez Rita , Nana Alissa L. , Spina Salvatore , Seeley William W. , Grinberg Lea T. , Karch Celeste M. 
Published: March 2023

AbstractTauopathies are a heterogenous group of neurodegenerative disorders characterized by tau aggregation in the brain. In a subset of tauopathies, rare mutations in theMAPTgene, which encodes the tau protein, are sufficient to cause disease; however, the events downstream ofMAPTmutations are poorly understood. Here, we investigate the role of long non-coding RNAs (lncRNAs), transcripts >200 nucleotides with low/no coding potential that regulate transcription and translation, and their role in tauopathy. Using stem cell derived neurons from patients carrying aMAPTp.P301L, IVS10+16, or p.R406W mutation, and CRISPR-corrected isogenic controls, we identified transcriptomic changes that occur as a function of theMAPTmutant allele. We identified 15 lncRNAs that were commonly differentially expressed across the threeMAPTmutations. The commonly differentially expressed lncRNAs interact with RNA-binding proteins that regulate stress granule formation. Among these lncRNAs,SNHG8was significantly reduced in a mouse model of tauopathy and in FTLD-tau, progressive supranuclear palsy, and Alzheimer’s disease brains. We show thatSNHG8interacts with tau and stress granule-associated RNA-binding protein TIA1. Overexpression of mutant tauin vitrois sufficient to reduceSNHG8expression and induce stress granule formation. RescuingSNHG8expression leads to reduced stress granule formation and reduced TIA1 levels, suggesting that dysregulation of this non-coding RNA is a causal factor driving stress granule formation via TIA1 in tauopathies.
ORF3c is expressed in SARS-CoV-2 infected cells and suppresses immune activation by inhibiting innate sensing
Authors: Müller Martin , Herrmann Alexandra , Fujita Shigeru , Uriu Keiya , Kruth Carolin , Strange Adam , Kolberg Jan E. , Schneider Markus , Ito Jumpei , Ensser Armin ,   The Genotype to Phenotype Japan (G2P-Japan) Consortium, Sato Kei , Sauter Daniel 
Published: March 2023

ABSTRACTSARS-CoV-2 proteins are translated from subgenomic RNAs (sgRNAs). While most of these sgRNAs are monocistronic, some viral mRNAs encode more than one protein. For example, theORF3asgRNA also encodes ORF3c, an enigmatic 4l-amino acid peptide. Here, we show that ORF3c is expressed in SARS-CoV-2 infected cells and suppresses RIG-I- and MDA5-mediated immune activation and IFN-β induction. Mechanistic analyses revealed that ORF3c interacts with the signaling adaptor MAVS, induces its C-terminal cleavage and inhibits the interaction of RIG-I with MAVS. The immunosuppressive activity of ORF3c is conserved among members of the subgenus sarbecovirus, including SARS-CoV and coronaviruses isolated from bats. Notably, however, the SARS-CoV-2 delta and kappa variants harbor premature stop codons in ORF3c demonstrating that this reading frame is not essential for efficient viral replicationin vivoand likely compensated by other viral proteins. In agreement with this, disruption of ORF3c did not significantly affect SARS-CoV-2 replication in CaCo-2 or CaLu-3 cells. In summary, we here identify ORF3c as an immune evasion factor of SARS-CoV-2 that suppresses innate sensing in infected cells.
Leveraging IFNγ/CD38 regulation to unmask and target leukemia stem cells in acute myelogenous leukemia
Authors: Murtadha Mariam , Park Miso , Zhu Yinghui , Caserta Enrico , Dona Ada Alice , Singer Mahmoud , Vahed Hawa , Tasndoh Theophilus , Gonzalez Asaul , Ly Kevin , Sanchez James F , Chowdhury Arnab , Pozhitkov Alex , Ghoda Lucy , Li Ling , Zhang Bin , Krishnan Amrita , Marcucci Guido , Williams John , Pichiorri Flavia 
Published: March 2023

ABSTRACTElimination of drug-resistant leukemia stem cells (LSCs) represents a major challenge to achieve a cure in acute myeloid leukemia (AML). Although AML blasts generally retain high levels of surface CD38 (CD38pos), the presence of CD34 and lack of CD38 expression (CD34posCD38neg) are immunophenotypic features of both LSC-enriched AML blasts and normal hematopoietic stem cells (HSCs). We report that IFN-γ induces CD38 upregulation in LSC-enriched CD34posCD38negAML blasts, but not in CD34posCD38negHSCs. To leverage the IFN-γ mediated CD38 up-regulation in LSCs for clinical application, we created a compact, single-chain CD38-CD3-T cell engager (CD38-BIONIC) able to direct T cells against CD38posblasts. Activated CD4posand CD8posT cells not only kill AML blasts but also produce IFNγ, which leads to CD38 expression on CD34posCD38negLSC-enriched blasts. These cells then become CD38-BIONIC targets. The net result is an immune-mediated killing of both CD38negand CD38posAML blasts, which culminates in LSC depletion.Statement of significanceThis work represents a potential advancement in the treatment of AML, as it involves the release of IFN-γ by T cells to induce CD38 expression and thus sensitizing leukemia stem cells, which have been resistant to current treatment regimens, to CD38-directed T cell engagers.