bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health Care Utilisation and Unmet Needs in Survivors of Head and Neck Cancer in Regional Australia: A Cross‐Sectional Survey
Authors: Pradhan Poorva , Sharman Ashleigh R. , Lacey Judith , Dwyer Patrick , Hill Jacques , Davis Kimberley J. , Craig Steven , Wu Raymond , Ashford Bruce , Mitchell Jenny , Clark Jonathan R. , Elliott Michael S. , Palme Carsten E. , Venchiarutti Rebecca L. 
Published: January 2025

ABSTRACTBackgroundThe post‐treatment survivorship period marks the transition away from acute care and poses distinct challenges for individuals with head and neck cancer (HNC). This can be especially challenging for people in regional areas who travel long distances to access care and experience unique challenges in accessing health services.AimTo investigate unmet needs and healthcare utilisation of survivors of HNC in regional areas.MethodsInvitations were sent to 619 survivors of HNC living in rural New South Wales, Australia, who were 1–15 years post‐treatment. Participants self‐reported unmet survivorship needs and the strength of these needs using the Cancer Survivors' Unmet Needs Measure. Health care utilisation over the preceding 12 months was collected using an investigator‐designed questionnaire.ResultsOne hundred and seventeen responses were received (19% response rate). Participants were predominantly male (65%), had oropharynx cancer (52%), with mean age of 70.2 years. Some 54% of participants reported at least one unmet need, and 40% rated these unmet needs as 'strong'. Top unmet needs included concern about recurrence (24%), access to local services (15%), and financial support (15%). 94% of participants reported seeing their GP, while 62% visited a dental clinic; only 10% sought professional psychosocial support despite prevalent unmet needs.ConclusionRural survivors of HNC in Australia have substantial unmet psychosocial needs yet demonstrate low utilisation of professional psychosocial support. This may reflect the limited availability or accessibility of services for this population, which could be addressed with shared models of care utilising both GP‐led and telehealth services.
Longitudinal Psychological Distress After Malignant Brain Tumor Diagnosis: A Multilevel Analysis of Patients and Their Caregivers
Authors: Karger André , Kisić Anna‐Maria , Quente Caterina , Klett Maike K. , Schäfer Ralf , Sabel Michael , Rapp Marion 
Published: January 2025

ABSTRACTObjectiveMalignant brain tumors are associated with debilitating symptoms and a poor prognosis, resulting in high psychological distress for patients and caregivers. There is a lack of longitudinal studies investigating psychological distress in this group. This study evaluated fear of progression (FoP), anxiety and depression in patients and their caregivers in the 6 months following malignant brain tumor diagnosis.MethodsThis prospective, observational study assessed FoP (FoP‐Q‐SF[P]), anxiety and depression (HADS) at diagnosis (T0) and after three (T1) and 6 months (T2) in patients with malignant brain tumors (primary, secondary) and their caregivers. Multilevel analyses were used to examine changes over time and differences between patients and caregivers, while accounting for the interdependence in their distress values.ResultsSeventy‐one patients and 68 caregivers were included in the analysis. Throughout the study period, over 50% reported clinically relevant FoP, almost 50% reported clinically relevant anxiety, and over 30% reported relevant depression. Over all time points, caregivers reported significantly higher anxiety and depression than patients. Anxiety decreased between T0 and T2 in both groups. Exploratory analyses showed that female sex was associated with higher anxiety, and older age with higher depression. No significant predictors were identified for FoP.ConclusionA substantial number of patients and caregivers experience clinically relevant psychological distress in the 6 months following a malignant brain tumor diagnosis. Caregivers are particularly distressed, reporting higher anxiety and depression. Integrating psycho‐oncological assessments and interventions for both patients and caregivers into clinical care is critical to address the psychological distress associated with malignant brain tumors.
Attraction of Bactrocera cucurbitae (Coquillett) to selected gut microbiota supernatants: implications for pest control
Authors: Liu Yanan , Tian Zhenya , Yang Jingfang , Zhou Zhongshi 
Published: January 2025

AbstractBACKGROUNDBactrocera cucurbitae (Coquillett) is a distructive quarantine insect pest that causes significant economic losses on cucurbit crops. To explore a green control approach, we investigated the behavioral responses of B. cucurbitae larvae and adults to bacterial suspensions, sediments, and supernatants derived from eight gut microbial strains across four distinct genera. The proboscis extension response was used to evaluate the impact of these microbial strains. In addition, using food selection experiments, two‐choice trap methods, and gas chromatography–mass spectrometry, we isolated and identified the predominant volatile compounds in the microbiota supernatants.RESULTSAmong the tested gut microbial strains, Kluyvera, Morganella, and Providencia exhibited notable attraction toward B. cucurbitae. In particular, the supernatant of Providencia M38 revealed the most highly attractive effect on B. cucurbitae larvae, whereas the supernatant of Morganella M72 was highly attractive to B. cucurbitae adults. Primary components present in the supernatant of M38 and M72 were dimethyl disulfide, indole, 2‐nonone, phenethyl alcohol, and 1‐decanol.CONCLUSIONStrains of M38 and M72 displayed remarkable attractive properties for B. cucurbitae larvae and adults, respectively, presenting promising potential for developing a novel attractant for this pest species. © 2025 Society of Chemical Industry.
Novel genomic features in entomopathogenic fungus Beauveria bassiana ILB308: accessory genomic regions and putative virulence genes involved in the infection process of soybean pest Piezodorus guildinii
Authors: Oberti Héctor , Sessa Lucia , Oliveira‐Rizzo Carolina , Di Paolo Andrés , Sanchez‐Vallet Andrea , Seidl Michael F. , Abreo Eduardo 
Published: January 2025

AbstractBACKGROUNDBiological control methods involving entomopathogenic fungi like Beauveria bassiana have been shown to be a valuable approach in integrated pest management as an environmentally friendly alternative to control pests and pathogens. Identifying genetic determinants of pathogenicity in B. bassiana is instrumental for enhancing its virulence against insects like the resistant soybean pest Piezodorus guildinii. This study focused on comparative genomics of different B. bassiana strains and gene expression analyses to identify virulence genes in the hypervirulent strain ILB308, especially in response to infection of P. guildinii and growth on hydrocarbon HC15, a known virulence enhancer.RESULTSStrain ILB308 showed the highest number of virulence‐related features, such as candidate virulence proteins, effectors, small secreted proteins and biosynthetic gene clusters. ILB308 also had a high percentage of unique DNA sequences, including six accessory scaffolds. Gene expression analysis at 4 days post inoculation revealed upregulation of known virulence factors, including Tudor domain proteins, LysM motif‐containing proteins, subtilisin‐like proteases and novel genes encoding secreted effectors and heat‐labile enterotoxins. Growth on HC15 led to the upregulation of genes associated with oxidoreductase activity related to cuticular alkane degradation and fermentation metabolism/antioxidant responses in the hemolymph. The low number of known B. bassiana virulence genes points to novel or unknown mechanisms acting on the interaction between P. guildinii and strain ILB308.CONCLUSIONThe presence of accessory genomic regions and unique virulence genes in ILB308 may contribute to its higher virulence. These genes could be considered as potential targets for enhancing fungal virulence through genetic manipulation. © 2025 Society of Chemical Industry.
Investigating non‐target site resistance to pyroxsulam in a glyphosate‐resistant Lolium rigidum population
Authors: Zeng Yalin , Liu Min , Qian Hong , Zhao Hui , Fang Yuhang , Yu Qin , Bai Lianyang , Pan Lang 
Published: January 2025

AbstractBACKGROUNDResistance to multiple herbicides is common in Lolium rigidum. Here, resistance to acetolactate synthase (ALS)‐ and susceptibility to acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides was confirmed in a glyphosate‐resistant L. rigidum population (NLR70) from Australia and the mechanisms of pyroxsulam resistance were examined.RESULTSNo ALS target‐site mutations nor gene overexpression were detected. Cytochrome P450 monooxygenase (P450) and glutathione S‐transferase (GST) inhibitors (indicators of some certain P450s or GSTs) did not significantly affect the resistance to pyroxsulam. Nevertheless, HPLC analysis showed that plants of the NLR70 population metabolized pyroxsulam faster than plants of the herbicide‐susceptible population (SVLR1). RNA sequencing analysis and RT‐qPCR validation confirmed that four P450s (CYP709B2, CYP72A14, CYP89A2, CYP94B3), one GT (UGT79), and one ABC transporter (ABCG41) genes were constitutively upregulated in NLR70 plants.CONCLUSIONThis study demonstrates that the glyphosate‐resistant L. rigidum population (NLR70) also exhibits resistance to pyroxsulam and identifies six candidate genes associated with non‐target site resistance to pyroxsulam. © 2025 Society of Chemical Industry.
Silencing of the plant‐derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED
Authors: Liu Yifan , Gong Cheng , Hu Yuan , Han Haolin , Tian Tian , Luo Yili , Yang Xin , Xie Wen , Wu Qingjun , Wang Shaoli , Guo Zhaojiang , Zhang Youjun 
Published: January 2025

AbstractBACKGROUNDThe whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability and reproductive capacity are linked to its ability to acquire and integrate horizontally transferred genes (HTGs) into its genome. These HTGs increase the physiological and metabolic capacities of this pest, including cholesterol synthesis, which is critical for its survival and reproductive success. Among these genes, we identified a plant‐derived B. tabaci Δ7‐sterol C5‐desaturase‐like gene (BtSC5DL), which plays a pivotal role in B. tabaci cholesterol metabolism and reproductive biology.RESULTSIn this study, we cloned and identified the BtSC5DL gene from B. tabaci Mediterranean (MED). Bioinformatics and molecular analyses revealed that BtSC5DL was transferred from plants to B. tabaci millions of years ago and is now stably expressed in this species. Silencing BtSC5DL through dsRNA feeding resulted in significant reductions in egg production and cholesterol content in B. tabaci MED. Furthermore, virus‐induced gene silencing (VIGS) experiments confirmed that long‐term suppression of BtSC5DL had a notable ability to control whitefly populations.CONCLUSIONOur results demonstrate the crucial role of BtSC5DL in cholesterol biosynthesis in B. tabaci MED and suggest that the acquisition of this gene significantly enhances the reproductive capacity of this species. These findings provide a theoretical basis for the development of RNA interference (RNAi)‐based pest control strategies targeting BtSC5DL, offering a potential new approach for the effective management of whitefly populations in agricultural settings. © 2025 Society of Chemical Industry.
Fungal effector genes involved in the suppression of chitin signaling as novel targets for the control of powdery mildew disease via a nontransgenic RNA interference approach
Authors: Bakhat Nisrine , Jiménez‐Sánchez Alejandro , Ruiz‐Jiménez Laura , Padilla‐Roji Isabel , Velasco Leonardo , Pérez‐García Alejandro , Fernández‐Ortuño Dolores 
Published: January 2025

AbstractBACKGROUNDChitin is a crucial component of fungal cell walls and an effective elicitor of plant immunity; however, phytopathogenic fungi have developed virulence mechanisms to counteract the activation of this plant defensive response. In this study, the molecular mechanism of chitin‐induced suppression through effectors involved in chitin deacetylases (CDAs) and their degradation (EWCAs) was investigated with the idea of developing novel dsRNA‐biofungicides to control the cucurbit powdery mildew caused by Podosphaera xanthii.RESULTSThe molecular mechanisms associated with the silencing effect of the PxCDA and PxEWCAs genes were first studied through dsRNA cotyledon infiltration assays, which revealed a ≈80% reduction in fungal biomass and a 50% decrease in gene expression. To assess the impact on powdery mildew disease control, in vitro and in planta assays were carried out in growth chamber and glasshouse experiments, with ≈50% reduction in disease symptoms after 8 days postinoculation (dpi) in leaf discs and 12 dpi in plants’ leaves, respectively. This control was extended for 21 days when the dsRNAs were protected on the carbon dot nanocarriers. Additionally, the uptake of the dsRNAs by fungal spores was observed 12 h postapplication via confocal microscopy, and efficient processing of dsRNAs into siRNAs by the melon RNAi machinery was observed 24 h postspraying through sRNA‐seq.CONCLUSIONSThis study highlights notable advancements in environmentally friendly disease management, and features the technological potential of RNA‐based fungicides together with nanotechnology for cucurbit powdery mildew control. © 2025 Society of Chemical Industry.
Tumor‐Associated Macrophages Nano‐Reprogrammers Induce “Gear Effect” to Empower Glioblastoma Immunotherapy
Authors: Wang Yang , Li Guangzhe , Su Jianlong , Liu Yiming , Zhang Xiaomai , Zhang Guanyi , Wu Zhihao , Li Jinrong , Wang Xu , Zhang Yuxuan , Bai Mingrui , Yao Yuanhang , Wang Ruimin , Shao Kun 
Published: January 2025

AbstractGlioblastoma (GBM), the most malignant brain tumor with high prevalence, remains highly resistant to the existing immunotherapies due to the significant immunosuppression within tumor microenvironment (TME), predominantly manipulated by M2‐phenotypic tumor‐associated macrophages (M2‐TAMs). Here in this work, an M2‐TAMs targeted nano‐reprogrammers, MG5‐S‐IMDQ, is established by decorating the mannose molecule as the targeting moiety as well as the toll‐like receptor (TLR) 7/8 agonist, imidazoquinoline (IMDQ) on the dendrimeric nanoscaffold. MG5‐S‐IMDQ demonstrated an excellent capacity of penetrating the blood‐brain barrier (BBB) as well as selectively targeting M2‐TAMs in the GBM microenvironment, leading to a phenotype transformation and function restoration of TAMs shown as heightened phagocytic activity toward tumor cells, enhanced cytotoxic effects, and improved tumor antigen cross‐presentation capability. In the meantime, by induction of a function‐oriented “gear effect”, MG5‐S‐IMDQ treatment extended its impact systemically by enhancing the infiltration of type I conventional dendritic cells (cDC1s) into the tumor sites and bolstering adaptive immune responses. In sum, by precisely working on M2‐TAMs as a unique target in tumor situ, the nano‐reprogrammers successfully established a robust immune network that worked synergistically to combat tumors. This facile nanoplatform‐based immunomodulatory strategy, serving as a powerful and convenient immune monotherapy or as a complementary treatment alongside other therapies like surgery, provided deep insights for advancing translational study in GBM.
Deciphering the Complexity of Step Profiles on Vicinal Si(001) Surfaces Through Multiscale Simulations
Authors: Li Pai , Zhao Chao , Liu Yun , Xue Zhongying , Ding Feng , Wei Xing 
Published: January 2025

AbstractThe behavior of vicinal Si(001) surfaces are a subject of intense research for years, yet the mechanism behind its step modulation remains unresolved. Step B, in particular, can meander randomly or form a periodic zigzag profile, a surface phenomenon that has eluded explanation due to the lack of appropriate simulation tools. Here, a multiscale simulation strategy, enhanced by machine learning potentials are proposed, to investigate this mesoscale behavior. The study reveals a phase transition in the step profile on vicinal Si(001) surfaces from random meandering to a zigzag wave pattern as the miscut angle decreases. This step‐profile transition is corroborated by Monte Carlo simulations and experimental observations. Remarkably, this transition is robust across various surface conditions, including bare, hydrogen‐saturated, boron‐doped, or strained surfaces. The findings resolve the long‐standing puzzle of step polymorphism on vicinal Si(001) surfaces and pave the way for exploring mesoscale phenomena using multiscale simulations.
Self‐Driving Microscopes: AI Meets Super‐Resolution Microscopy
Authors: Ward Edward N. , Scheeder Anna , Barysevich Max , Kaminski Clemens F. 
Published: January 2025

AbstractThe integration of Machine Learning (ML) with super‐resolution microscopy represents a transformative advancement in biomedical research. Recent advances in ML, particularly deep learning (DL), have significantly enhanced image processing tasks, such as denoising and reconstruction. This review explores the growing potential of automation in super‐resolution microscopy, focusing on how DL can enable autonomous imaging tasks. Overcoming the challenges of automation, particularly in adapting to dynamic biological processes and minimizing manual intervention, is crucial for the future of microscopy. Whilst still in its infancy, automation in super‐resolution can revolutionize drug discovery and disease phenotyping leading to similar breakthroughs as have been recognized in this year's Nobel Prizes for Physics and Chemistry.
Co‐assemblies of Silver Nanoclusters and Fullerenols With Enhanced Third‐Order Nonlinear Optical Response
Authors: Li Jinrui , Xie Huiyan , Sun Di , Li Hongguang , Xin Xia 
Published: January 2025

AbstractExploring potential third‐order nonlinear optical (NLO) materials attracts ever‐increasing attention. Given that the atomically precise and rich adjustable structural features of silver nanoclusters (Ag NCs), as well as the unique π‐electron conjugated system of carbon‐based nanomaterials, a supramolecular co‐assembly amplification strategy to enhance the luminescent intensity and NLO performance of the hybrids of the two components, are constructed and the relationship between structures and optical properties are investigated. By combining water soluble Ag NCs [(NH4)6[Ag6(mna)6] (H2mna = 2‐mercaptonicotinic acid, abbreviated to Ag6─NCs hereafter) containing uncoordinated carboxyl groups with water‐soluble fullerene derivatives modified with multiple hydroxyl groups (fullerenols, C60─OH), the π‐electron delocalization is expanded owing to non‐covalent hydrogen bonding effect between Ag6─NCs and C60─OH, which provides a feasible basis for realizing the NLO response. Then, the co‐assemblies are doped into a PMMA matrix to prepare composite film and its NLO properties are evaluated by Z‐scan technique. Remarkably, the effective nonlinear absorption coefficients β is of two orders magnitude higher than those of the Ag6─NCs assemblies at the absence of C60─OH. This work showcases a new approach for amplifying NLO responses which greatly facilitates the development of integrated photonic devices.
Friedrich Bleich (1878–1950) – Beratender Ingenieur für Stahlbau, Autor, Schriftleiter und Weltbürger
Authors: Pelke Eberhard , Kurrer Karl‐Eugen 
Published: January 2025

AbstractFriedrich Bleich (1878–1950) war ein österreichischer Bauingenieur jüdischer Herkunft. Als einer der wenigen Ingenieure seiner Zeit gelang es ihm, in der Konsolidierungsperiode des Stahlbaus (1900–1950) die statisch‐konstruktive Praxis auf einzigartige Weise mit wissenschaftlichem Arbeiten zu verschmelzen. Kommunikation und fachlicher Austausch der Ingenieure über nationale Schranken hinaus war stets das Anliegen Bleichs. Er zählte zu den Gründern der International Association for Bridge and Structural Engineering (IABSE) im Jahr 1928 und war deren wissenschaftlicher Sekretär bis 1938. Nach dem erzwungenen Anschluss Österreichs an das nationalsozialistische Deutschland und der sofort beginnenden systematischen Ausgrenzung der jüdischen Bevölkerung verließ der Weltbürger Bleich seine Heimat. Nach zweieinhalbjährigem Asyl in der Schweiz nahm die USA den international renommierten Bauingenieur 1941 auf. Im Alter von 63 Jahren stand Bleich vor einem Neuanfang seines Berufslebens, das er als wissenschaftlich beratender Ingenieur meisterte. In den USA erreichte sein wissenschaftliches Schaffen seinen Höhepunkt. Der Beitrag stellt Leben und Werk von Friedrich Bleich vor dem zeitgeschichtlichen Hintergrund in Europa und den USA dar.
High Volume Plasma Exchange Improves Survival Rates in Surgical Critically Ill Patients With Medical Jaundice and Hepatic Failure: A Comparative Study
Authors: Wu Shih‐Chi , Cheng Chih‐Chung , Yeh Hung‐Chieh , Cheng Han‐Tsung , Wang Yu‐Chun , Tzeng Chia‐Wei , Hsu Chia‐Hao , Muo Chih‐Hsin 
Published: January 2025

ABSTRACTObjectivesAcute liver failure poses a significant challenge in surgical critically ill patients. Treatments typically focus on physiological support and alleviation of hepatic insult. This study aims to evaluate the role of high‐volume plasma exchange (HVPE) in surgical critically ill patients with medical jaundice and hepatic failure.MethodA retrospective review was conducted on surgical critically ill patients with hepatic failure unresponsive to conventional therapy, excluding those with obstructive jaundice. HVPE was considered for patients with persistent hyperbilirubinemia (> 10 mg/dL) and coexisting conditions such as coagulopathy, hyperammonemia, more than Grade II hepato‐encephalopathy, or exacerbated sepsis/septic shock status or multiple organ failure. Patients were categorized into standard medical treatment (SMT) and SMT + HVPE groups. Demographics and laboratory data were collected for analysis.ResultA total of 117 patients were enrolled, with 79 in the SMT group and 38 in the SMT + HVPE group. There were no significant differences in laboratory data and MELD score upon admission. Before treatment, patients in the SMT + HVPE group exhibited higher levels of T‐bil., D‐bil., and sugar than the SMT group. After treatment, the SMT + HVPE group showed lower serum D‐bil. and AST levels but higher levels of albumin and platelets compared to the SMT group. The SMT + HVPE group demonstrated significantly lower delta T‐bil., delta D‐bil., and higher delta platelet levels. The survival rate was 31.6% (12/38) in the SMT + HVPE group and 1.3% (1/79) in the SMT group. The in‐hospital mortality rate in the SMT + HVPE group was lower than that in the SMT group, with a hazard ratio of 0.42 in the crude model and 0.34 (95% CI = 0.20–0.60 and p = 0.0002) in the adjusted model.ConclusionOur findings suggest that HVPE improves survival rates in surgical critically ill patients with medical jaundice and hepatic failure. However, due to its retrospective nature, further studies were warranted.
Understanding Norms, Norm Clusters and Robustness at Different Levels of Global Governance
Authors: Funke Carolin , Dijkzeul Dennis 
Published: January 2025

AbstractThis chapter develops a theoretical framework and methods for studying disability inclusion at different levels of global governance based on different strands of IR norm theory. It also discusses the methods of data collection and analysis for each level of global governance and develops a multi-level perspective that goes beyond traditional International Law and IR approaches to understand the (limited) impact of disability inclusion in humanitarian crises so far.