bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential of multi-strain probiotics extract as an anti-inflammatory agent through inhibition of macrophage migration inhibitory factor activity
Authors: Nyotohadi Denny , Go Tjie Kok 
Published: January 2023

Background: Probiotics are reported to have a role for improving health conditions and reduce the risk of diseases associated with inflammation. However, how they affect inflammation has not been well studied. The inflammation occurs during the progression of chronic diseases could damage the normal function of cells, tissues, and organs. Macrophage migration inhibitory factor (MIF) is recognized as a cytokine playing a key role in the inflammation process. Inhibition of its activity has been used by researchers as an approach for alleviating the inflammation. This study aims to evaluate the potential inhibitory effect of the extract of multi-strain probiotics consisting of Lactobacillus casei EMRO 002, L. casei EMRO 213, L. plantarum EMRO 009, L. fermentum EMRO 211, L. rhamnosus EMRO 014, L. bulgaricus EMRO 212,and Rhodopseudomonas palustris EMRO 201 on MIF tautomerase activity, the reversibility, and the mechanism of inhibition. Hence, the scope of this study is the evaluation of the functional characteristics of multi-strain probiotics extract on MIF activity.Methods:The multi-strain probiotics were centrifuged and the supernatant was separated from the pellet. The supernatant, i.e. the extract, was mixed with MIF and analyzed for its inhibition effect on MIF tautomerase activity. The reversibility of inhibition was evaluated by preincubation and dilution assays, and the mechanism of inhibition was determined by kinetic evaluation.Results:The multi-strain probiotics extract inhibited MIF tautomerase activity with an IC50 of 7.80 ± 1.96 mg/L. The preincubation and dilution assays showed that the inhibition is reversible, and the kinetic evaluation predicted that the extract components might bind to the enzyme active site and other site(s) of MIF.Conclusion:The findings show that the extract was able to inhibit MIF tautomerase activity reversibly and its components might bind to the enzyme active site and other site(s) of MIF suggest that the multi-strain probiotics extract has potential to be used as an anti-inflammatory agent for the treatment of chronic diseases associated with inflammation.Keywords: Multi-strain probiotics, anti-inflammatory, macrophage migration inhibitory factor, reversible inhibition, kinetic evaluation.
АNTIMETASTATIC EFFECT OF B. SUBTILIS IMV B-7724 LECTIN OBSERVED IN LEWIS LUNG CARCINOMA MODEL
Authors: Fedosova N.I. , Symchych T.V. , Cheremshenko N.L. , Chumak A.V. , Koval E.V. , Karaman O.M. , Voyeykova I.M. 
Published: January 2023

Aim: To study the antitumor and antimetastatic effects of B. subtilis IMV B-7724 lectin used in neoadjuvant and adjuvant settings in vivo. Materials and Methods: Studies were performed on C57Bl/6J mice; Lewis lung carcinoma (LLC) was used as an experimental tumor. В. subtilis ІМV В-7724 lectin was administered to tumor-bearing mice or to mice which underwent surgical resection of the primary tumor. The lectin was injected subcutaneously, 10 times, at a single dose of 5 or 1 mg/kg of body weight. The standard indicators of tumor growth and metastasis were evaluated. Results: Independently of the application settings, the lectin at a dose of 1 mg/kg of b.w. caused more pronounced effect than at a dose of 5 mg/kg of b.w. The administration of B. subtilis IMV B-7724 lectin to the mice with LLC in neoadjuvant setting did not cause notable antitumor effect but led to a significant decrease in the number and volume of lung metastases. The lectin administration in adjuvant setting significantly inhibited metastasis: the metastasis inhibition index reached 63.0% and 100% in the mice treated with the lectin at a dose of 5 mg/kg and 1 mg/kg respectively. The mean survival time of the treated animals significantly increased. Conclusion: A pronounced antimetastatic effect of B. subtilis IMV B-7724 lectin administered in an adjuvant setting was demonstrated.
CLINICAL CASE OF NON-TYPICAL METASTASIS OF NEUROENDOCRINE LUNG CARCINOMA TO THE VASCULAR MEMBRANE OF THE EYE
Authors: Zakhartseva L.M. , Chytaieva H.E. , Artemov O.V. 
Published: January 2023

Background: Metastases in eye structures are rare (1–5% cases at systemic spread of different malignancies, mainly breast and lung cancers). The prognosis is poor. The overall survival usually does not exceed 12 or even 6 months. If metastases are found in the choroid membrane, the probability that the patient has multiple metastatic lesions of other organs increases significantly. Lung neuroendocrine neoplasms are rare (1–2% of all malignancies in adults), but mainly aggressive tumors. They are cha­racterized by “blurred”, nonspecific clinical symptoms, the correct diagnosis is delayed seriously, and distant metastases are seen in more than 40% of patients (usually in chest structures, liver, bones, brain, and adrenal glands; metastasis to vascular membrane of the eye ranks the 6th place). Case report: Own clinical observation of a male patient with rare metastasis of lung neuroendocrine carcinoma to the choroid of the left eye is presented. The disease is manifested by an ocular metastasis, which was initially considered an embryonic tumor. Other metastatic lesions (hilar lymph nodes, liver, soft tissues) were detected on computed tomography a little bit later. The diagnostic algorithm using routine histological examination and immunohistochemistry, including detection of neuroendocrine markers (chromogranin A, synaptophysin), cytokeratin 7 and Ki-67 expression in primary and metastatic tumors is described.
PRIMARY CUTANEOUS ADENOID CYSTIC CARCINOMA IN THE TRUNK: CASE REPORT AND LITERATURE REVIEW
Authors: Moreno M. , De Oliveira A. , Czarnobai I. , Cássia Boff T. 
Published: January 2023

Primary cystic adenoid skin carcinoma is a rare and poorly documented neoplasm in literature worldwide, with just over 250 reports. This work describes a 52-year-old male patient, with no comorbidities, who presented this neoplasm in nodular format in the posterior thoracic region, associated with localized pain and erythema — symptoms that led him to seek medical help. The clinical findings, differential diagnosis and treatment particularities were reviewed and correlated with the clinical case. The choice of type of surgical treatment was done considering the characteristics of the primary lesion that are associated with a worse prognosis. Despite its rarity, this neoplasm is easily identified through histological examination, the correct choice of treatment and patient follow-up, essential to increase survival. Thus, this work contributes to diminish the scarcity of literature related to this topic, especially the form of treatment employed.
CHEMORESISTANCE RELATED TO HYPOXIA ADAPTATION IN MESOTHELIOMA CELLS FROM TUMOR SPHEROIDS
Authors: Endoh D. , Ishii K. , Kohno K. , Virgona N. , Miyakoshi Y. , Yano T. , Ishida T. 
Published: January 2023

Background: Hypoxia has been noted as a key factor for induction and maintenance of cancer stemness thereby leading to therapy resistance. Three-dimensional (3D) spheroid models demonstrate a heterogeneity of hypoxic regions replicating the in vivo situation within tumors. Utilizing an established 3D spheroid model, we investigated whether extrinsic hypoxia reinforced chemoresistance in malignant pleural mesothelioma (MPM) spheroids. Materials and Methods: Tumor spheres were generated from Meso-1 (a typical human MPM cell line) cells having high spheroid-forming ability. To induce hypoxia condition, we utilized a hypoxia chamber with regulation of O2 and CO2 levels. Cell viability was estimated by a WST-8 assay. Real-time polymerase chain reaction and Western blot were performed to evaluate the expression at mRNA and protein levels. Results: Compared with cells cultured in the two-dimensional monolayer model, tumor sphere cells showed elevated mRNA levels of cancer stemness markers (CD26, CD44 and ABCG2) and protein levels of the stemness and hypoxia adaptation markers (ABCG2, ALDH1A1 and HIFs). Correlating with this, 3D spheroid cells were more resistant to permetrexed and topotecan than the two-dimensional cells, indicative of their potential for hypoxic adaptation. Furthermore, significantly stronger resistance to both chemotherapeutic agents was observed in spheroid cells upon hypoxic challenge compared to spheroid cells under normoxia. Conclusion: From the present data, it is concluded that hypoxia adaptation of MPM cells from tumor spheres could enhance their chemoresistance.
ONCOLYTIC ACTIVITY OF HUMAN ORTHOPNEUMOVIRUS IN CANCER CELL LINES
Authors: Aziz I.M. , Bhat R. , Farrag M.A. , Almajhdi F.N. 
Published: January 2023

Oncolytic virotherapy is an emerging biotherapeutic platform for selectively infecting cancer cells and triggering apoptosis in a number of malignant cells due to robust viral replication. Studies related to the oncolytic activity of human orthopneumovirus (hOPV) are conflicting. Aim: This study was designed to elucidate the possible role of hOPV in the modulation of cell growth and apoptosis in cancer cell lines including human epidermoid carcinoma (HEp-2), lung epithelial cell line (A549), and breast cancer cell line (MCF-7). Materials and Methods: The oncolytic activity of hOPV on cancer cells was studied in vitro. The virus titers were determined by tissue culture infectious dose (TCID50/mL) in A549 cell. The cytotoxic effect of the virus on HEp-2, A549, and MCF-7 was determined using MTT and trypan blue dye exclusion test assays. hOPV in the infected cells was detected using real-time reverse transcription polymerase chain reaction (rRT-PCR) and indirect immunofluorescence (IIF) assays. The relative expression of apoptosis-related genes (CASP-3, -8, -9, Bax, Bcl-2, Bcl-XL, TP53, P21) during virus infection was estimated using rRT-PCR assay in comparison with the house-keeping gene (GAPDH). Results: hOPV infection inhibited the growth of HEp-2, A549, and MCF-7 cells in a dose-and time-dependent manner. At a multiplicity of infection (MOI) of 5, hOPV reduced the viability of A549 cells to about 16%, HEp-2 to 22%, and MCF-7 to 28% (p = 0.001), while no significant inhibitory effect was observed when cells were infected at MOI of 1 and 2. hOPV mRNA and antigens were detected in infected HEp-2, A549, and MCF-7 cells by RT-PCR and IIF. Upon hOPV infection, expression of CASP-3, -8, -9, as well as Bax, TP53, and p21 mRNA increased while expression of Bcl-2, Bcl-xL anti-apoptotic genes decreased. In hOPV-infected A549 cells, the fold increase of CASP-8 and CASP-9, Bax, TP53, and P21 expression exceeded significantly compared to that in HEp-2 or MCF-7 cells. Conclusions: Our results provide evidence that hOPV could be a potential candidate for oncolytic virotherapy.
ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205
Authors: Garmanchuk L.V. , Vedenicheva N.P. , Al-Maali G.A. , Ostapchenko D.I. , Tseyslyer Yu.V. , Liashenko V.A. , Bisko N.A. , Kosakivska I.V. , Ostapchenko L.I. 
Published: January 2023

Background: The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts. Aim: To determine the antiproliferative effect of crude extracts and cytokinin fractions from the mycelial biomass of seven fungi species on colon cancer cells in vitro. Materials and Methods: Cytokinin content in mycelial biomass of Ganoderma lucidum, Lentinula edodes, Trametes versicolor, Pleurotus ostreatus, Morchella esculenta, Hericium coralloides, and Fomitopsis officinalis was determined by high performance liquid chromatography mass spectrometry. The antiproliferative effect of the mushroom extracts on the human colon adenocarcinoma Colo 205 cells was assessed by MTT-test. Results: The content of cytokinins (trans-zeatin, zeatin riboside, isopentenyladenosine, isopentenyladenine and zeatin-O-glucoside) was determined in the mycelial biomass of the medicinal macromycetes. Zeatin-type hormones prevailed in all species, though trans-zeatin was the most abundant in H. coralloides and M. esculenta. In P. ostreatus, only zeatin-O-glucoside was detected. The lowest IC50 was found for both the cytokinin fraction (0.21 μg/ml) and the crude extract (0.17 μg/ml) from mycelial biomass of H. coralloides. F. officinalis also demonstrated high antiproliferative effect against Colo 205 cells: IC50 was 0.9 μg/ml for the crude extract and almost twice lower for the cytokinin fraction. In the studied concentration range (0.016–2 μg/ml), the crude extracts from G. lucidum and M. esculenta and the cytokinin fraction from L. edodes did not reach IC50 values. Conclusions: The present study showed that crude extracts and/or cytokinin fractions of several medicinal Basidiomycetes species are capable to inhibit proliferation of colon cancer cells in vitro. Crude extract cytotoxicity of H. coralloides, P. ostreatus and T. versicolor was higher than that of cytokinin fraction while antiproliferative effect of cytokinin fraction from F. officinalis was higher than that in its crude extract.
Designing of non circular air intakes for subsonic gas-turbine engines
Authors: Tsukanov Ruslan 
Published: January 2023

The subject matter of the article is the process of subsonic air intake shaping for gas-turbine engines at the airplane preliminarily design stage. The goal is to develop a mathematical model for non-circular air intake shaping for gas-turbine engines on the base of V. I. Polikovskii method of subsonic air intake shaping for high-bypass ratio turbofan. The tasks to be solved are: to consider the possibility of non-circular shape of the external outline of the engine nacelle; to take into account the possibility of non-circular shape of the internal air intake duct (in the first approximation, the shape of internal air intake duct cross-section is defined in the form of a rectangular with possible four different radiuses in its corners); to consider the engine inlet spinner presence. The methods used are: analytical and digital mathematical methods, implemented in MathCAD and Microsoft Visual Studio systems. The following results were obtained: On the base of the proposed method, new calculation module for the Power Unit software version 11.8 has been developed (С-language Win32 UNICODE application) having a friendly user interface. Conclusions. The scientific novelty of the results obtained is as follows: 1) mathematical model (algorithm and its program implementation) for non-circular air intake shaping for gas-turbine engines has been developed considering non-circular shape of the external outline of the engine nacelle, non-circular shape of the air intake duct internal outline, presence of engine inlet spinner, and zero expansion angle in the diffuser outlet cross-section; 2) adequacy of calculation results by the developed mathematical model is shown by means of comparison with the shape of real air intake, developed by the Antonov Company. For the following improvement of the mathematical model, it is desirable to add the possibility of considering S‑shape of the air intake duct, defining its length from designer’s considerations, defining a bigger radius of curvature of the air intake lip, and considering the presence of boundary layer bleeding devices in front of the air intake.
Застосування ДКП-фільтрації для обробки сигналів інерційного вимірювального модулю
Authors: Abramov Sergiy , Abramova Victoriya , Abramov Klavdiy , Lukin Volodymyr 
Published: January 2023

This article is devoted to the analysis and processing of signals of inertial measuring modules used as part of inertial navigation systems or in research measuring complexes for conducting shock tests. The modules have a set of sensors for measuring the speed of the object, its orientation in space, the gravitational forces with which it moves, as well as the magnetic field surrounding it. Using the example of a typical inertial measurement module WT901SDCL, it is shown that the signals of acceleration, angular velocity, and angular position generated by the module have a certain fluctuation component, which deteriorates the accuracy of determining the estimated parameters, as well as to the appearance of an accumulated error in the determination of coordinates of the inertial navigation system. In such conditions, it is advisable to use secondary processing methods, namely, filtering methods. Since the information component of the signals of the inertial measurement module has significant dynamics, one of the key requirements for the filtering method is its preservation. However, a filter is also required to effectively suppress the fluctuation component. Among the currently existing filters, the filter based on discrete cosine transform (DCT-filter) has the best trade-off according to these requirements. It is shown that the use of this filter allows reducing the intensity of the noise component in the information signal by the average of 1.4 times the value of the mean square error and, accordingly, to reduce the measurement errors of physical parameters. Simultaneously, the shape of the signals after applying the DCT-filter remains almost unchanged, all sharp dynamic changes in the signal are preserved, and the absolute levels of the signals also remain the same. Thus, the use of DCT-filtering for signal post-processing in inertial measurement modules can be considered quite reasonable.
Roman Law on legal personality: pro et contra
Authors: Zhornokui Yu. M. 
Published: January 2023

The article is devoted to the study and comparison of the teachings of Roman lawyers regarding the emergence and understanding of the essence of a legal entity as a certain organization of people, as well as providing arguments “for” and “against” its existence as an independent participant in private relations. Attention is drawn to the fact that the current state of the development of civilist thought regarding the development of the category “legal entity” by Roman lawyers indicates the existence of three approaches: 1) Roman lawyers developed and used a legal structure that was different from the citizens of Rome and could participate in the civil turnover of that time; 2) the category of legal entity was not known to Roman lawyers, but the main features characterised to its modern understanding (legal capacity, property separation, the possibility to participate in legal proceedings, etc.) were developed precisely in Ancient Rome; 3) for the jurisprudence of the Roman period, the category “legal entity” or its individual characteristics were not known in the sense that are now attached to the content of the relevant characteristics, but the use of individual constructions, such as “universitas”, “corpus”, “collegia”, “sodalitia”, etc., was intended to show that in private relations, property can exist without being tied to a Roman citizen as a subject of rights.
The analysis of the civil law doctrine of Roman law regarding the doctrine of a legal entity in the context of comparison with the modern understanding of the essence of such a participant in civil relations led to the conclusion that although Roman lawyers did not develop the concept of a legal entity, they proposed its main practical manifestation: the concept of legal capacity, which does not depend on an individual, the concept of legal capacity and the main types of legal entities that were further developed in European jurisprudence (corporations and institutions). The merit of Roman law is the substantiation of the legal way by which a variety of social entities could be introduced into civil turnover.
It is emphasized that although the concept of “legal entity” was not defined by the Roman ancient lawyers, and its essence was not directly studied by them, the idea of expanding the circle of subjects of private law at the expense of special organizations, unions of citizens belongs to the Roman private law. It can be assumed that the very concept of “legal entity” at this stage of historical development was not formed from the point of view of substantive law, but was formed only as a social category, but not as a legal one.
PENGARUH PENGETAHUAN PAJAK, EFEKTIVITAS SISTEM PERPAJAKAN, DAN MOTIVASI PAJAK TERHADAP KEPATUHAN WAJIB PAJAK ORANG PRIBADI
Authors: Safitri Rizma Nadiah , Afiqoh Nyimas Wardatul 
Published: January 2023

Penelitian ini bertujuan untuk mengetahui Pengaruh Pengetahuan Pajak, Efektivitas Sistem dan Motivasi Pajak tehadap kepatuhan Wajib Pajak orang pribadi. Penelitian ini menggunakan metode penelitian kuantitatif. Populasi dalam penelitian ini adalah seluruh Wajib Pajak orang pribadi yang tedaftar di KPP Pratama Gresik. Sampel yang digunakan dalam penelitian ini berjumlah 95 orang. Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah Accidential Sampling. Metode pengumpulan data yanng menggunakan kuesioner. Teknik analisis yang digunakan dalam penelitian ini, Analisis Regresi Linier Berganda. Berdasarkan hasil analisis yang dilakukan diperoleh kesimpulan bahwa Pengetahuan perpajakan tidak berpengaruh terhadap kepatuhan Wajib Pajak orang pribadi. Sedangkan pada Efektivitas sistem perpajakan dan Motivasi Pajak berpengaruh positif terhadap Kepatuhan Wajib Pajak orang pribadi.
МЕТОДОЛОГІЧНІ ЗАСАДИ ДОСЛІДЖЕННЯ НОРМОТВОРЧОЇ ДІЯЛЬНОСТІ МІНІСТЕРСТВА ВНУТРІШНІХ СПРАВ УКРАЇНИ
Authors: Чернякович Є. А. 
Published: January 2023

Наукове обґрунтування будь-якої проблематики неможливе без методологічного дослідження, тому у статті досліджується найбільш важливі групи методів предмету нашого дослідження які складають: історичний метод сприятиме вивченню еволюції наукового осмислення та методології дослідження нормотворчої діяльності загалом та Міністерства внутрішніх справ України зокрема; системно-структурний метод дозволить висвітлити сутності нормотворчої діяльності Міністерства внутрішніх справ України як важливого елемента механізму правотворення; функціональний метод сприятиме дослідженню завдань нормотворчої діяльності Міністерства внутрішніх справ України та напрямів її вдосконалення; герменевтичний метод – для вивчення та інтерпретації текстів нормативно-правових актів; порівняльно-правовий методи забезпечить виявлення спільного та відмінного у нормотворчій діяльності Міністерств внутрішніх справ інших країн; спеціально-юридичний метод дозволить охарактеризувати стадії, юридичну техніку та види нормативно-правових актів нормотворчої діяльності Міністерства внутрішніх справ України; формально-юридичний метод дозволяє встановити сутнісні ознаки нормотворчості, виробити поняття нормотворчої діяльності Міністерства внутрішніх справ України. Завданням методу теоретико-правового прогнозування полягає у з’ясуванні, як і в якому напрямі розвиватиметься досліджуваний об’єкт (відповідні суспільні відносини). Цей метод забезпечує обґрунтування наукових прогнозів розвитку нормотворчої діяльності Міністерства внутрішніх справ України, можливої зміни природи і сутності цього різновиду нормотворчості, підвищення ефективності актів відомчої нормотворчості та їх ролі в процесі управління суспільством.