bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Search of a “Newer and Truer” Literature: Thomas Phillips Thompson and The Labor Advocate
Authors: Buchanan David 
Published: September 2023

This article describes the uses of literature in The Labor Advocate: A Weekly Labor Reform Newspaper (Toronto, 1890-91). As editor of the Advocate, Thomas Phillips Thompson aimed to increase awareness of the means and consequences of industrial capitalism, and thus enhance the possibility of social justice for the working class. He did so in a mixed format periodical that included poetry, short fiction, and serialized novels as well as editorials, biographies, obituaries, reports, letters, and columns. Over forty-four issues, Thompson experimented with literary expression to attract readers and foster the democratic reform of social organization. Analysis of the Advocate points to the importance of communication strategies in both the early history of the Canadian labour press and the longer history of labour in transnational contexts.
Kolaborasi Karya Tari Suara Hati
Authors: Supendi Eko , Sri Kuncoro Jonet , Suroto Suroto 
Published: September 2023

Research on art creation entitled Suara Hati Dance Collaboration involving dancers (students) and children with visual impairments (disabilities) to answer concerns about how a dancer feels dancing in the dark. This research method is a collaboration between the potential of dancers and the artistic potential of blind children. The dancers’ explorations were inspired by the behavior of the character Gendari in the Mahabarata story, who closed his eyes all his life only to feel the same darkness as his husband, Destrarastra. Exploring the potential of blind children is an important part of this research to determine the process and form of work. The results of this study provide an artistic experience for dancers, moving in the dark just following their auditory instincts. Providing space for expression and artistic experience for blind children, fostering self-confidence, courage, and a sense of pride in being able to work with dancers and appear in performances that are shown publicly.Keywords: voice, heart, collaboration, visual impairment, Gendari, movement, darkness.
Workshop Peran Siswa sebagai Warga Negara Dalam Menyikapi Realitas di Masa Pandemi
Authors: Hubi Zindan Baynal , Pangestu Ilham Aji , Adhari Nursanda Rizki , Fahmi Rizal 
Published: September 2023

Pelaksanaan Pengabdian Kepada Masyarakat (PKM) ini bertujuan diantaranya pertama menumbuhkan kecerdasan dan kecakapan kewarganegaraan terkait dengan peran warga negara muda menghadapi pandemi Covid-19, kedua kecakapan dan kesadaran kewarganegaraan tersebut diimplementasikan ketika mampu bersikap efektif didalam tindakan serta mampu mengaktualisasikan nilai-nilai Pancasila dalam menghadapi pandemi Covid-19, dan ketiga mitra mampu berperan dalam menyikapi realitas dimasa pandemi termasuk dengan kebajikan yang dilakukan. Mitra dalam PKM ini adalah para siswa SMAN 20 Kabupaten Tangerang. Metode pelaksanaan yang digunakan yaitu penyuluhan dalam bentuk ceramah melalui media googlemeet. Dalam PKM ini materi yang disampaikan meliputi kesadaran bela negara ditengah pandemi, aktualisasi nilai Pancasila menjadi relevan dengan situasi hari ini, dan bagaimana siswa dapat berperan serta dalam menyikapi realitas dimasa pandemi. Adapun kesimpulan dalam materi ini menekankan pada konsep triple helix concept, dimana pemerintah dengan political power, akademisi/ pelajar dengan knowledge power dan masyarakat dengan social control, harus senantiasa bersinergi bersama dalam menanggulangi pandemi ini. Dampak dengan diadakannya workshop kegiatan terhadap Siswa SMAN 20 Kabupaten Tangerang, tidak lain sedikitnya merupakan salah satu upaya untuk menumbuhkan kecakapan kewarganegaraan utamanya terhadap warga negara muda. Kecakapan warga negara itu harus ditimbulkan dan disadarkan melalui pendidikan ataupun contoh nyata yang dilakukan berbagai elemen bangsa. Adapun rekomendasi terhadap pelaksnaan PKM ini kedepan diantaranya adalah pertama, adanya kegiatan lanjutan yang melibatkan komponen masyarakat, kedua, melakukan pendampingan secara langsung dengan memperhatikan protokol kesehatan, dan ketiga, singkronisasi yang lebih luas khususnya instansi pemerintah.
Teh Daun Kelor Sebagai Inovasi Dan Peningkatan Nutrisi Pangan Khas Indonesia
Authors: Mulyana Deden , Listiana Elis , Lestary Deasy , Bintari Vivi Indah 
Published: September 2023

Pandemi Covid-19 memiliki dampak pada semua sektor, sehingga dirasakan pula oleh warga kampung Anaka, Kelurahan Urug, Kecamatan Kawalu, Kota Tasikmalaya. Pada tahun 2020, para pemuda sekitar melihat potensi alam yang indah, sehingga berinisiatif untuk menyulap sebuah bukit menjadi lokasi wisata alam baru bernama Bukit Nangela dengan pemandangan Kawasan hutan milik perhutani. Dengan rencana jangka panjang untuk menjadi Kampung Wisata Anaka. Namun, perlu adanya ciri khas kuliner tertentu yang akan menaikan trend positif berkunjung masyarakat. Mata pencaharian utama di Kelurahan Urug adalah sebagai buruh tani. Terdapat hasil pertanian yang masih belum terolah oleh masyarakat untuk dijadikan produk unggulan yaitu daun kelor. Masyarakat belum mengetahui bagaimana cara pengolahan daun kelor agar dapat dikonsumsi dengan bantuan teknologi secara sederhana. Untuk menyelesaikan permasalahan tersebut, maka kegiatan pengabdian masyarakat dengan Skema Ketahanan Pangan (PbM-KP) telah dirancang solusinya yaitu 1) Pendampingan dan pemberian biaya untuk sarana produksi pengolahan teh daun kelor, 2) Pelatihan pengolahan daun kelor menjadi teh daun kelor siap jual, dan 3) Pemasaran produk pertanian dengan membuatkan kemitraan dengan pedagang kuliner disekitar bukit nangela sehingga menjadi produk unggulan desa wisata dan melakukan pemasaran secara online. Tujuan dari kegiatan ini adalah produksi olahan daun kelor yaitu teh daun kelor siap dipasarkan, sehingga menjadi produk olahan unggulan di Kampung Anaka Keluarahan Urug. Berdasarkan hasil kegiatan yang dilakukan bahwa masyarakat antusias dan memanfaatkan teh daun kelor menjadi minuman olahan.
Pengembangan Usaha Pudot Bergizi Sebagai Upaya Penguatan Ekonomi Mandiri Ibu PKK
Authors: Putri Ronasari Mahaji , Fitasari Eka , Putri Sri Andika , Devi Hilda Mazarina 
Published: September 2023

PKK RT 06 RW 09 merupakan salah satu perkumpulan ibu yang berada di daerah Landungsari Malang, beranggotakan 40 ibu. Sebanyak 2 dari 40 ibu telah memiliki usaha sampingan dirumah yakni berupa toko perancangan, dan catering. Sedangkan ibu yang lainnya ada yang bekerja di luar rumah, dan ada yang sebagai ibu rumah tangga. Sebagian besar ibu di daerah tersebut mempunyai putra putri yang masih kecil dan sangat mendukung kegiatan yang dapat menambah keahlian dan ketrampilan ibu sebagai upaya untuk meningkatkan gizi keluarganya, termasuk juga dapat meningkatkan perekonomian keluarga. Banyaknya waktu ibu saat di rumah memungkinkan para ibu untuk lebih berkreasi, baik yang mendatangkan materi ataupun tidak, namun memberikan manfaat bagi keluarganya. Pengabdi mengambil kesempatan untuk dapat memberikan manfaat bagi Ibu PKK RT 06 RW 09 Landungsari. Solusi yang ditawarkan oleh pengabdi adalah membuat usaha puding sedot bergizi, yang relatif diminati oleh pangsa pasar, dengan biaya yang murah, bahan baku yang mudah untuk diakses, serta manfaat yang didapatkan dengan berproduksi pudot yakni memberikan gizi yang baik untuk keluarga dan calon konsumen. Kegiatan pengabdian masyarakat berupa pelatihan usaha pudot (puding sedot) telah berjalan di bulan Maret 2022 dan diharapkan menjadi salah satu ketrampilan yang dikuasai ibu ibu PKK sebagai bekal dalam membangun ekonomi mandiri ibu RT.
Overcoming extended lag phase on optically pure lactic acid production from pretreated softwood solids
Authors: Campos Joana , Almqvist Henrik , Bao Jie , Wallberg Ola , Lidén Gunnar 
Published: September 2023

Optically pure lactic acid (LA) is needed in PLA (poly-lactic acid) production to build a crystalline structure with a higher melting point of the biopolymer than that of the racemic mixture. Lignocellulosic biomass can be used as raw material for LA production, in a non-food biorefinery concept. In the present study, genetically engineered P. acidilactici ZP26 was cultivated in a simultaneous saccharification and fermentation (SSF) process using steam pretreated softwood solids as a carbon source to produce optically pure D-LA. Given the low concentrations of identifiable inhibitory compounds from sugar and lignin degradation, the fermentation rate was expected to follow the rate of enzymatic hydrolysis. However, added pretreated solids (7% on weight (w/w) of water-insoluble solids [WIS]) significantly and immediately affected the process performance, which resulted in a long lag phase (more than 40 h) before the onset of the exponential phase of the fermentation. This unexpected delay was also observed without the addition of enzymes in the SSF and in a model fermentation with glucose and pretreated solids without added enzymes. Experiments showed that it was possible to overcome the extended lag phase in the presence of pretreated softwood solids by allowing the microorganism to initiate its exponential phase in synthetic medium, and subsequently adding the softwood solids and enzymatic blend to proceed to an SSF with D-LA production.
Integration of white rot mushroom cultivation to enhance biogas production from oil palm kernel pulp by solid-state digestion
Authors: Panngoen Pathompong , Leksawasdi Noppol , Rachtanapun Pornchai , Chakrabandhu Yasinee , Jinsiriwanit Siriwat 
Published: September 2023

Solid-state fermentation is one of the promising technologies for biogas production because of its low water footprint and solid output which is potentially used in fuel or agricultural applications. Oil palm kernel pulp (OPKP) is a by-product generated from the extraction of palm kernel oil from the mesocarp of the oil palm tree and usually contains a large amount of lignocellulose and moderate protein content, which makes it suitable for use as a mushroom substrate. Cultivation of white rot mushrooms on lignocellulose may enhance its biodegradation by biodelignification. In this study, the incorporation of the cultivation of edible white rot mushrooms, Pluerotus ostreatus and Pleurotus pulmonarius, to enhance biogas production by solid-state digestion was studied. The biological efficiency of mushroom production from the OPKP substrate of P. ostreatus and P. pulmonarius was 49.81% ± 11.28% and 46.94% ± 13.49%, respectively, corresponding to the substrate weight loss of 15.87% and 13.92%. After 30 days, methane yield obtained through the solid-state digestion of P. ostreatus- and P. pulmonarius-treated OPKP substrates was increased to 98.11 mL/gVS (191%) and 101.10 mL/gVS (197%), respectively, compared with the untreated OPKP substrate. In consideration of energy loss during the biological conversion, the calorific values of the OPKP substrate, P. ostreatus-treated OPKP substrate, and P. pulmonarius-treated OPKP substrate were 11.03 ± 0.71 kJ/g, 9.30 ± 0.23 kJ/g, and 8.83 ± 0.70 kJ/g, respectively, while those of the digestion residues of P. ostreatus and P. pulmonarius-treated OPKP substrates were 8.45 ± 0.13 kJ/g and 8.55 ± 0.11 kJ/g, respectively.
SWAT model-based quantification of the impact of land use land cover change on sediment yield in the Fincha watershed, Ethiopia
Authors: Regasa Motuma Shiferaw , Nones Michael 
Published: September 2023

In recent years, Ethiopia is facing problems due to soil erosion, mainly because of the conversion of natural vegetation into cultivated land to answer to increasing human pressure. Such changes in coverage are fostering soil erosion, which is also affecting dam reservoirs, because of the increasing sediment yield entering the lake. The present study focuses on the Ethiopian Fincha watershed and takes advantage of the potentiality of the Soil and Water Assessment Tool (SWAT) model, to investigate how land use land cover changes impact soil erosion and the consequent sediment yield entering the Fincha Dam. The SWAT model was calibrated and validated using time series data of stream flow and sediment covering the period 1986-2008, and its performance was evaluated by looking at the coefficient of determination, Nash-Sutcliffe simulation efficiency, and per cent bias. Once validated, the SWAT model was applied to derive sediment yield for the future 30 years, based on forecasted land use land cover conditions. The results show that the mean annual soil loss rate increased from 32.51 t ha−1 in 1989 to 34.05 t ha−1 in 2004, reaching 41.20 t ha−1 in 2019. For the future, a higher erosion risk should be expected, with the annual soil loss rate forecasted to be 46.20 t ha−1 in 2030, 51.19 t ha−1 in 2040, and 53.98 t ha−1 in 2050. This soil erosion means that sediments transported to the Fincha Dam, located at the watershed outlet, increased significantly in the last 30 years (from 1.44 in 1989 to 2.75 mil t in 2019) and will have the same trend in the future (3.08–4.42 mil t in 2020 and 2050, respectively), therefore highly affecting the Fincha reservoir services in terms of reduction of water volume for irrigation and hydroelectric power generation. By providing possible medium/long-term scenarios, the present work can help land planners and decision-makers in developing future land management strategies based on actual projections, eventually aiming to reduce soil erosion at the watershed scale and guarantee the sustainable development of the Fincha region and its key hydraulic infrastructures.
ABCA4 c.6480-35A>G, a novel branchpoint variant associated with Stargardt disease
Authors: Rodríguez-Hidalgo María , de Bruijn Suzanne E. , Corradi Zelia , Rodenburg Kim , Lara-López Araceli , Valverde-Megías Alicia , Ávila-Fernández Almudena , Fernandez-Caballero Lidia , Del Pozo-Valero Marta , Corominas Jordi , Gilissen Christian , Irigoyen Cristina , Cremers Frans P. M. , Ayuso Carmen , Ruiz-Ederra Javier , Roosing Susanne 
Published: September 2023

Introduction: Inherited retinal dystrophies (IRDs) can be caused by variants in more than 280 genes. The ATP-binding cassette transporter type A4 (ABCA4) gene is one of these genes and has been linked to Stargardt disease type 1 (STGD1), fundus flavimaculatus, cone–rod dystrophy (CRD), and pan-retinal CRD. Approximately 25% of the reported ABCA4 variants affect RNA splicing. In most cases, it is necessary to perform a functional assay to determine the effect of these variants.Methods: Whole genome sequencing (WGS) was performed in one Spanish proband with Stargardt disease. The putative pathogenicity of c.6480-35A&gt;G on splicing was investigated both in silico and in vitro. The in silico approach was based on the deep-learning tool SpliceAI. For the in vitro approach we used a midigene splice assay in HEK293T cells, based on a previously established wild-type midigene (BA29) containing ABCA4 exons 46 to 48.Results: Through the analysis of WGS data, we identified two candidate variants in ABCA4 in one proband: a previously described deletion, c.699_768+342del (p.(Gln234Phefs*5)), and a novel branchpoint variant, c.6480-35A&gt;G. Segregation analysis confirmed that the variants were in trans. For the branchpoint variant, SpliceAI predicted an acceptor gain with a high score (0.47) at position c.6480-47. A midigene splice assay in HEK293T cells revealed the inclusion of the last 47 nucleotides of intron 47 creating a premature stop codon and allowed to categorize the variant as moderately severe. Subsequent analysis revealed the presence of this variant as a second allele besides c.1958G&gt;A p.(Arg653His) in an additional Spanish proband in a large cohort of IRD cases.Conclusion: A splice-altering effect of the branchpoint variant, confirmed by the midigene splice assay, along with the identification of this variant in a second unrelated individual affected with STGD, provides sufficient evidence to classify the variant as likely pathogenic. In addition, this research highlights the importance of studying non-coding regions and performing functional assays to provide a conclusive molecular diagnosis.
Plasma YKL-40 is associated with prognosis in patients with metastatic pancreatic cancer receiving immune checkpoint inhibitors in combination with radiotherapy
Authors: Johansen Astrid Z. , Novitski Sif I. , Hjaltelin Jessica X. , Theile Susann , Boisen Mogens K. , Brunak Søren , Madsen Daniel H. , Nielsen Dorte L. , Chen Inna M. 
Published: September 2023

BackgroundYKL-40, also known as chitinase-3-like protein 1 (CHI3L1), is a secreted glycoprotein produced by various cell types including stromal, immune, and cancer cells. It contributes to cancer progression through tumor-promoting inflammation and has been shown to inhibit the cytotoxicity of T and NK lymphocytes. In vivo studies have demonstrated synergistic anti-cancer effects of blocking YKL-40 in combination with immune checkpoint inhibitors (ICIs). Biomarkers for the prediction of the response to ICIs are highly needed. We investigated the association between plasma YKL-40 and clinical benefit and survival in patients with metastatic pancreatic cancer (mPC) receiving ICIs and stereotactic body radiotherapy (SBRT).MethodsBlood samples were collected from 84 patients with mPC who participated in the randomized phase II CheckPAC study, in which patients received nivolumab with or without ipilimumab combined with a single fraction of SBRT. Plasma YKL-40 was measured using a commercial ELISA kit.ResultsElevated baseline plasma YKL-40 was an independent predictor of shorter overall survival (OS) (HR 2.19, 95% CI 1.21–3.95). A ≥ 40% decrease in plasma YKL-40 during treatment was associated with longer progression-free survival (p = 0.009) and OS (p = 0.0028). There was no correlation between plasma YKL-40 and the tumor burden marker CA19-9 at baseline or during treatment.ConclusionThis study contributes new knowledge regarding YKL-40 as a predictor of clinical benefit from ICIs and radiotherapy. These exploratory results warrant further investigation of YKL-40 as a biomarker for patients treated with immunotherapies.Clinical trial registrationClinicaltrials.gov, identifier NCT02866383.
Epithelial NAD+ depletion drives mitochondrial dysfunction and contributes to intestinal inflammation
Authors: Novak Elizabeth A. , Crawford Erin C. , Mentrup Heather L. , Griffith Brian D. , Fletcher David M. , Flanagan Meredith R. , Schneider Corinne , Firek Brian , Rogers Matthew B. , Morowitz Michael J. , Piganelli Jon D. , Wang Qian , Mollen Kevin P. 
Published: September 2023

IntroductionWe have previously demonstrated that a pathologic downregulation of peroxisome proliferator-activated receptor–gamma coactivator 1-alpha (PGC1α) within the intestinal epithelium contributes to the pathogenesis of inflammatory bowel disease (IBD). However, the mechanism underlying downregulation of PGC1α expression and activity during IBD is not yet clear.MethodsMice (male; C57Bl/6, Villincre/+;Pgc1afl/fl mice, and Pgc1afl/fl) were subjected to experimental colitis and treated with nicotinamide riboside. Western blot, high-resolution respirometry, nicotinamide adenine dinucleotide (NAD+) quantification, and immunoprecipitation were used to in this study.ResultsWe demonstrate a significant depletion in the NAD+ levels within the intestinal epithelium of mice undergoing experimental colitis, as well as humans with ulcerative colitis. While we found no decrease in the levels of NAD+-synthesizing enzymes within the intestinal epithelium of mice undergoing experimental colitis, we did find an increase in the mRNA level, as well as the enzymatic activity, of the NAD+-consuming enzyme poly(ADP-ribose) polymerase-1 (PARP1). Treatment of mice undergoing experimental colitis with an NAD+ precursor reduced the severity of colitis, restored mitochondrial function, and increased active PGC1α levels; however, NAD+ repletion did not benefit transgenic mice that lack PGC1α within the intestinal epithelium, suggesting that the therapeutic effects require an intact PGC1α axis.DiscussionOur results emphasize the importance of PGC1α expression to both mitochondrial health and homeostasis within the intestinal epithelium and suggest a novel therapeutic approach for disease management. These findings also provide a mechanistic basis for clinical trials of nicotinamide riboside in IBD patients.
Optimizing postbiotic production through solid-state fermentation with Bacillus amyloliquefaciens J and Lactiplantibacillus plantarum SN4 enhances antibacterial, antioxidant, and anti-inflammatory activities
Authors: Tong Yucui , Guo He'nan , Abbas Zaheer , Zhang Jing , Wang Junyong , Cheng Qiang , Peng Shuyue , Yang Tiantian , Bai Ting , Zhou Yichen , Li Jinzhuan , Wei Xubiao , Si Dayong , Zhang Rijun 
Published: September 2023

BackgroundPostbiotics are an emerging research interest in recent years and are fairly advanced compared to prebiotics and probiotics. The composition and function of postbiotics are closely related to fermentation conditions.MethodsIn this study, we developed a solid-state fermentation preparation method for postbiotics with antimicrobial, antioxidant, and anti-inflammatory activities. The antibacterial activity was improved 3.62 times compared to initial fermentation conditions by using optimization techniques such as single factor experiments, Plackett–Burman design (PBD), steepest ascent method (SAM), and central composite design (CCD) methods. The optimized conditions were carried out with an initial water content of 50% for 8 days at 37°C and fermentation strains of Bacillus amyloliquefaciens J and Lactiplantibacillus plantarum SN4 at a ratio of 1:1 with a total inoculum size of 8%. The optimized SSF medium content ratios of peptide powder, wheat bran, corn flour, and soybean meal were 4, 37.4, 30, and 28.6%, respectively.ResultsUnder these optimized conditions, postbiotics with a concentration of 25 mg/mL showed significant broad-spectrum antibacterial capabilities against Escherichia coli, Salmonella, and Staphylococcus aureus and strong antioxidant activity against ABTS, DPPH, and OH radicals. Moreover, the optimized postbiotics exhibited good anti-inflammatory ability for reducing nitric oxide (NO) secretion in RAW 264.7 macrophage cells in response to LPS-induced inflammation. Furthermore, the postbiotics significantly improved intestinal epithelial wound healing capabilities after mechanical injury, such as cell scratches in IPEC-J2 cells (p &lt; 0.05).ConclusionIn brief, we developed postbiotics through optimized solid-state fermentation with potential benefits for gut health. Therefore, our findings suggested that the novel postbiotics could be used as potential functional food products for improving body health.