bluuebunny/crossref_metadata_2025_split huggingface dataset:
Work Resilience Scale
Authors: Liu Doudou , Meng Xue , Li Chaoping , Xie Songke 
Published: June 2023

Abstract: This study aims to investigate the psychometric properties of the WRS-C (Work Resilience Scale – Chinese version) through two studies. In Study 1 (Sample 1: N = 463), we conducted an exploratory factor analysis (EFA) and identified the two-factor solution representing work resilience after translating the Work Resilience Scale (WRS) into Chinese. In Study 2, the psychometric properties of the WRS-C were investigated in two samples (Sample 2: N = 477; Sample 3: N = 374). The confirmatory factor analysis (CFA) confirms a two-factor structure. Furthermore, the WRS-C shows measurement invariance across groups by gender, age, job tenure, and education level. Furthermore, the results provide evidence for the convergent and concurrent validity of the WRS-C. In addition, its predictive validity has been demonstrated through its associations with mental health outcomes, performance outcomes, and attitude outcomes. Overall, the WRS-C is a reliable and valid instrument in the Chinese context, which can be utilized in future research and practice.
Childhood sexual abuse is associated with higher total ghrelin serum levels in adulthood: results from a large, population-based study
Authors: Wittekind Dirk Alexander , Kratzsch Jürgen , Mergl Roland , Wirkner Kerstin , Baber Ronny , Sander Christian , Witte A. Veronica , Villringer Arno , Kluge Michael 
Published: June 2023

AbstractGhrelin is an orexigenic peptide hormone synthesized in times of stress and hunger and alterations of the ghrelin system following acute stressors could be repeatedly shown in humans. However, little data exists on long-term effects of trauma on the ghrelin system. We aimed to investigate the influence of childhood trauma on total ghrelin serum levels in a large, population-based study. Total serum ghrelin was measured in 1666 participants of a population-based cross-sectional study (‘LIFE study’). The Childhood Trauma Screener (CTS) was used for the assessment of childhood trauma in the final sample (n = 1086; mean age: 57.10 ± 16.23 years; 632 males, 454 females). Multiple linear regression analyses and generalized linear models were chosen to examine the association between childhood trauma and total serum ghrelin concentrations. Childhood sexual abuse went along with significantly higher ghrelin serum levels in the total sample (β = 0.114, t = 3.958; p = 0.00008) and in women (β = 0.142, t = 3.115; p = 0.002), but not in men (β = 0.055; t = 1.388; p = 0.166). Women with severe emotional neglect in the childhood had higher ghrelin levels than those without (odds ratio = 1.204; p = 0.018). For the CTS Sum Score and other CTS sub-scale scores, no significant association with ghrelin serum levels was found. Our study is the first to show associations between childhood sexual trauma and total ghrelin levels in adults in a large, community-based sample. Our results should initiate further research of the role of ghrelin in human stress response in prospective study designs.
Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice
Authors: Jiang Xue , Cheng Yu , Zhu Yuzhang , Xu Caoling , Li Qiaodan , Xing Xuemei , Li Wenqing , Zou Jiaqi , Meng Lan , Azhar Muhammad , Cao Yuzhu , Tong Xianhong , Qin Weibing , Zhu Xiaoli , Bao Jianqiang 
Published: June 2023

AbstractIn mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function of N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation by sculpting the maternal transcriptome through timely degradation of poly(A) tail mRNAs. This is achieved through the ac4C deposition on the key CCR4-NOT complex transcripts. Importantly, we devise a method for examining the poly(A) tail length (PAT), termed Hairpin Adaptor-poly(A) tail length (HA-PAT), which outperforms conventional methods in terms of cost, sensitivity, and efficiency. In summary, these findings provide genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development.
Trispecific antibody targeting HIV-1 and T cells activates and eliminates latently-infected cells in HIV/SHIV infections
Authors: Promsote Wanwisa , Xu Ling , Hataye Jason , Fabozzi Giulia , March Kylie , Almasri Cassandra G. , DeMouth Megan E. , Lovelace Sarah E. , Talana Chloe Adrienna , Doria-Rose Nicole A. , McKee Krisha , Hait Sabrina Helmold , Casazza Joseph P. , Ambrozak David , Beninga Jochen , Rao Ercole , Furtmann Norbert , Birkenfeld Joerg , McCarthy Elizabeth , Todd John-Paul , Petrovas Constantinos , Connors Mark , Hebert Andrew T. , Beck Jeremy , Shen Junqing , Zhang Bailin , Levit Mikhail , Wei Ronnie R. , Yang Zhi-yong , Pegu Amarendra , Mascola John R. , Nabel Gary J. , Koup Richard A. 
Published: June 2023

AbstractAgents that can simultaneously activate latent HIV, increase immune activation and enhance the killing of latently-infected cells represent promising approaches for HIV cure. Here, we develop and evaluate a trispecific antibody (Ab), N6/αCD3-αCD28, that targets three independent proteins: (1) the HIV envelope via the broadly reactive CD4-binding site Ab, N6; (2) the T cell antigen CD3; and (3) the co-stimulatory molecule CD28. We find that the trispecific significantly increases antigen-specific T-cell activation and cytokine release in both CD4+ and CD8+ T cells. Co-culturing CD4+ with autologous CD8+ T cells from ART-suppressed HIV+ donors with N6/αCD3-αCD28, results in activation of latently-infected cells and their elimination by activated CD8+ T cells. This trispecific antibody mediates CD4+ and CD8+ T-cell activation in non-human primates and is well tolerated in vivo. This HIV-directed antibody therefore merits further development as a potential intervention for the eradication of latent HIV infection.
Tetraspanin-8 sequesters syntaxin-2 to control biphasic release propensity of mucin granules
Authors: Wojnacki José , Lujan Agustin Leonardo , Brouwers Nathalie , Aranda-Vallejo Carla , Bigliani Gonzalo , Rodriguez Maria Pena , Foresti Ombretta , Malhotra Vivek 
Published: June 2023

AbstractAgonist-mediated stimulated pathway of mucin and insulin release are biphasic in which rapid fusion of pre-docked granules is followed by slow docking and fusion of granules from the reserve pool. Here, based on a cell-culture system, we show that plasma membrane-located tetraspanin-8 sequesters syntaxin-2 to control mucin release. Tetraspanin-8 affects fusion of granules during the second phase of stimulated mucin release. The tetraspanin-8/syntaxin-2 complex does not contain VAMP-8, which functions with syntaxin-2 to mediate granule fusion. We suggest that by sequestering syntaxin-2, tetraspanin-8 prevents docking of granules from the reserve pool. In the absence of tetraspanin-8, more syntaxin-2 is available for docking and fusion of granules and thus doubles the quantities of mucins secreted. This principle also applies to insulin release and we suggest a cell type specific Tetraspanin/Syntaxin combination is a general mechanism regulating the fusion of dense core granules.
Hypoxia induced responses are reflected in the stromal proteome of breast cancer
Authors: Kjølle Silje , Finne Kenneth , Birkeland Even , Ardawatia Vandana , Winge Ingeborg , Aziz Sura , Knutsvik Gøril , Wik Elisabeth , Paulo Joao A. , Vethe Heidrun , Kleftogiannis Dimitrios , Akslen Lars A. 
Published: June 2023

AbstractCancers are often associated with hypoxia and metabolic reprogramming, resulting in enhanced tumor progression. Here, we aim to study breast cancer hypoxia responses, focusing on secreted proteins from low-grade (luminal-like) and high-grade (basal-like) cell lines before and after hypoxia. We examine the overlap between proteomics data from secretome analysis and laser microdissected human breast cancer stroma, and we identify a 33-protein stromal-based hypoxia profile (33P) capturing differences between luminal-like and basal-like tumors. The 33P signature is associated with metabolic differences and other adaptations following hypoxia. We observe that mRNA values for 33P predict patient survival independently of molecular subtypes and basic prognostic factors, also among low-grade luminal-like tumors. We find a significant prognostic interaction between 33P and radiation therapy.
Defining diurnal fluctuations in mouse choroid plexus and CSF at high molecular, spatial, and temporal resolution
Authors: Fame Ryann M. , Kalugin Peter N. , Petrova Boryana , Xu Huixin , Soden Paul A. , Shipley Frederick B. , Dani Neil , Grant Bradford , Pragana Aja , Head Joshua P. , Gupta Suhasini , Shannon Morgan L. , Chifamba Fortunate F. , Hawks-Mayer Hannah , Vernon Amanda , Gao Fan , Zhang Yong , Holtzman Michael J. , Heiman Myriam , Andermann Mark L. , Kanarek Naama , Lipton Jonathan O. , Lehtinen Maria K. 
Published: June 2023

AbstractTransmission and secretion of signals via the choroid plexus (ChP) brain barrier can modulate brain states via regulation of cerebrospinal fluid (CSF) composition. Here, we developed a platform to analyze diurnal variations in male mouse ChP and CSF. Ribosome profiling of ChP epithelial cells revealed diurnal translatome differences in metabolic machinery, secreted proteins, and barrier components. Using ChP and CSF metabolomics and blood-CSF barrier analyses, we observed diurnal changes in metabolites and cellular junctions. We then focused on transthyretin (TTR), a diurnally regulated thyroid hormone chaperone secreted by the ChP. Diurnal variation in ChP TTR depended on Bmal1 clock gene expression. We achieved real-time tracking of CSF-TTR in awake TtrmNeonGreen mice via multi-day intracerebroventricular fiber photometry. Diurnal changes in ChP and CSF TTR levels correlated with CSF thyroid hormone levels. These datasets highlight an integrated platform for investigating diurnal control of brain states by the ChP and CSF.
Specific post-translational modifications of soluble tau protein distinguishes Alzheimer’s disease and primary tauopathies
Authors: Kyalu Ngoie Zola Nathalie , Balty Clémence , Pyr dit Ruys Sébastien , Vanparys Axelle A. T. , Huyghe Nicolas D. G. , Herinckx Gaëtan , Johanns Manuel , Boyer Emilien , Kienlen-Campard Pascal , Rider Mark H. , Vertommen Didier , Hanseeuw Bernard J. 
Published: June 2023

AbstractTau protein aggregates in several neurodegenerative disorders, referred to as tauopathies. The tau isoforms observed in post mortem human brain aggregates is used to classify tauopathies. However, distinguishing tauopathies ante mortem remains challenging, potentially due to differences between insoluble tau in aggregates and soluble tau in body fluids. Here, we demonstrated that tau isoforms differ between tauopathies in insoluble aggregates, but not in soluble brain extracts. We therefore characterized post-translational modifications of both the aggregated and the soluble tau protein obtained from post mortem human brain tissue of patients with Alzheimer’s disease, cortico-basal degeneration, Pick’s disease, and frontotemporal lobe degeneration. We found specific soluble signatures for each tauopathy and its specific aggregated tau isoforms: including ubiquitination on Lysine 369 for cortico-basal degeneration and acetylation on Lysine 311 for Pick’s disease. These findings provide potential targets for future development of fluid-based biomarker assays able to distinguish tauopathies in vivo.
A global meta-analysis of soil organic carbon in the Anthropocene
Authors: Beillouin Damien , Corbeels Marc , Demenois Julien , Berre David , Boyer Annie , Fallot Abigail , Feder Frédéric , Cardinael Rémi 
Published: June 2023

AbstractAnthropogenic activities profoundly impact soil organic carbon (SOC), affecting its contribution to ecosystem services such as climate regulation. Here, we conducted a thorough review of the impacts of land-use change, land management, and climate change on SOC. Using second-order meta-analysis, we synthesized findings from 230 first-order meta-analyses comprising over 25,000 primary studies. We show that (i) land conversion for crop production leads to high SOC loss, that can be partially restored through land management practices, particularly by introducing trees and incorporating exogenous carbon in the form of biochar or organic amendments, (ii) land management practices that are implemented in forests generally result in depletion of SOC, and (iii) indirect effects of climate change, such as through wildfires, have a greater impact on SOC than direct climate change effects (e.g., from rising temperatures). The findings of our study provide strong evidence to assist decision-makers in safeguarding SOC stocks and promoting land management practices for SOC restoration. Furthermore, they serve as a crucial research roadmap, identifying areas that require attention to fill the knowledge gaps concerning the factors driving changes in SOC.
Interaction-driven transport of dark excitons in 2D semiconductors with phonon-mediated optical readout
Authors: Chand Saroj B. , Woods John M. , Quan Jiamin , Mejia Enrique , Taniguchi Takashi , Watanabe Kenji , Alù Andrea , Grosso Gabriele 
Published: June 2023

AbstractThe growing field of quantum information technology requires propagation of information over long distances with efficient readout mechanisms. Excitonic quantum fluids have emerged as a powerful platform for this task due to their straightforward electro-optical conversion. In two-dimensional transition metal dichalcogenides, the coupling between spin and valley provides exciting opportunities for harnessing, manipulating, and storing bits of information. However, the large inhomogeneity of single layers cannot be overcome by the properties of bright excitons, hindering spin-valley transport. Nonetheless, the rich band structure supports dark excitonic states with strong binding energy and longer lifetime, ideally suited for long-range transport. Here we show that dark excitons can diffuse over several micrometers and prove that this repulsion-driven propagation is robust across non-uniform samples. The long-range propagation of dark states with an optical readout mediated by chiral phonons provides a new concept of excitonic devices for applications in both classical and quantum information technology.
Occurrence and backtracking of microplastic mass loads including tire wear particles in northern Atlantic air
Authors: Goßmann Isabel , Herzke Dorte , Held Andreas , Schulz Janina , Nikiforov Vladimir , Georgi Christoph , Evangeliou Nikolaos , Eckhardt Sabine , Gerdts Gunnar , Wurl Oliver , Scholz-Böttcher Barbara M. 
Published: June 2023

AbstractFew studies report the occurrence of microplastics (MP), including tire wear particles (TWP) in the marine atmosphere, and little data is available regarding their size or sources. Here we present active air sampling devices (low- and high-volume samplers) for the evaluation of composition and MP mass loads in the marine atmosphere. Air was sampled during a research cruise along the Norwegian coast up to Bear Island. Samples were analyzed with pyrolysis-gas chromatography-mass spectrometry, generating a mass-based data set for MP in the marine atmosphere. Here we show the ubiquity of MP, even in remote Arctic areas with concentrations up to 37.5 ng m−3. Cluster of polyethylene terephthalate (max. 1.5 ng m−3) were universally present. TWP (max. 35 ng m−3) and cluster of polystyrene, polypropylene, and polyurethane (max. 1.1 ng m−3) were also detected. Atmospheric transport and dispersion models, suggested the introduction of MP into the marine atmosphere equally from sea- and land-based emissions, transforming the ocean from a sink into a source for MP.
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy
Authors: Grosch Markus , Schraft Laura , Chan Adrian , Küchenhoff Leonie , Rapti Kleopatra , Ferreira Anne-Maud , Kornienko Julia , Li Shengdi , Radke Michael H. , Krämer Chiara , Clauder-Münster Sandra , Perlas Emerald , Backs Johannes , Gotthardt Michael , Dieterich Christoph , van den Hoogenhof Maarten M. G. , Grimm Dirk , Steinmetz Lars M. 
Published: June 2023

AbstractDilated cardiomyopathy is the second most common cause for heart failure with no cure except a high-risk heart transplantation. Approximately 30% of patients harbor heritable mutations which are amenable to CRISPR-based gene therapy. However, challenges related to delivery of the editing complex and off-target concerns hamper the broad applicability of CRISPR agents in the heart. We employ a combination of the viral vector AAVMYO with superior targeting specificity of heart muscle tissue and CRISPR base editors to repair patient mutations in the cardiac splice factor Rbm20, which cause aggressive dilated cardiomyopathy. Using optimized conditions, we repair >70% of cardiomyocytes in two Rbm20 knock-in mouse models that we have generated to serve as an in vivo platform of our editing strategy. Treatment of juvenile mice restores the localization defect of RBM20 in 75% of cells and splicing of RBM20 targets including TTN. Three months after injection, cardiac dilation and ejection fraction reach wild-type levels. Single-nuclei RNA sequencing uncovers restoration of the transcriptional profile across all major cardiac cell types and whole-genome sequencing reveals no evidence for aberrant off-target editing. Our study highlights the potential of base editors combined with AAVMYO to achieve gene repair for treatment of hereditary cardiac diseases.
Tracing metal footprints via global renewable power value chains
Authors: Fu Rao , Peng Kun , Wang Peng , Zhong Honglin , Chen Bin , Zhang Pengfei , Zhang Yiyi , Chen Dongyang , Liu Xi , Feng Kuishuang , Li Jiashuo 
Published: June 2023

AbstractThe globally booming renewable power industry has stimulated an unprecedented interest in metals as key infrastructure components. Many economies with different endowments and levels of technology participate in various production stages and cultivate value in global renewable power industry production networks, known as global renewable power value chains (RPVCs), complicating the identification of metal supply for the subsequent low-carbon power generation and demand. Here, we use a multi-regional input-output model (MRIO) combined with a value chain decomposition model to trace the metal footprints (MFs) and value-added of major global economies’ renewable power sectors. We find that the MFs of the global renewable power demand increased by 97% during 2005—2015. Developed economies occupy the high-end segments of RPVCs while allocating metal-intensive (but low value-added) production activities to developing economies. The fast-growing demand for renewable power in developed economies or developing economies with upper middle income, particularly China, is a major contributor to the embodied metal transfer increment within RPVCs, which is partly offset by the declining metal intensities in developing economies. Therefore, it is urgent to establish a metal-efficient and green supply chain for upstream suppliers as well as downstream renewable power installers for just transition in the power sector across the globe.
A 13-million turnover-number anionic Ir-catalyst for a selective industrial route to chiral nicotine
Authors: Yin Congcong , Jiang Ya-Fei , Huang Fanping , Xu Cong-Qiao , Pan Yingmin , Gao Shuang , Chen Gen-Qiang , Ding Xiaobing , Bai Shao-Tao , Lang Qiwei , Li Jun , Zhang Xumu 
Published: June 2023

AbstractDeveloping catalysts with both useful enantioselectivities and million turnover numbers (TONs) for asymmetric hydrogenation of ketones is attractive for industrial production of high-value bioactive chiral entities but remains a challenging. Herein, we report an ultra-efficient anionic Ir-catalyst integrated with the concept of multidentate ligation for asymmetric hydrogenation of ketones. Biocatalysis-like efficacy of up to 99% ee (enantiomeric excess), 13,425,000 TON (turnover number) and 224 s−1 TOF (turnover frequency) were documented for benchmark acetophenone. Up to 1,000,000 TON and 99% ee were achieved for challenging pyridyl alkyl ketone where at most 10,000 TONs are previously reported. The anionic Ir-catalyst showed a novel preferred ONa/MH instead of NNa/MH bifunctional mechanism. A selective industrial route to enantiopure nicotine has been established using this anionic Ir-catalyst for the key asymmetric hydrogenation step at 500 kg batch scale, providing 40 tons scale of product.
Accounting for forest condition in Europe based on an international statistical standard
Authors: Maes Joachim , Bruzón Adrián G. , Barredo José I. , Vallecillo Sara , Vogt Peter , Rivero Inés Marí , Santos-Martín Fernando 
Published: June 2023

AbstractCovering 35% of Europe’s land area, forest ecosystems play a crucial role in safeguarding biodiversity and mitigating climate change. Yet, forest degradation continues to undermine key ecosystem services that forests deliver to society. Here we provide a spatially explicit assessment of the condition of forest ecosystems in Europe following a United Nations global statistical standard on ecosystem accounting, adopted in March 2021. We measure forest condition on a scale from 0 to 1, where 0 represents a degraded ecosystem and 1 represents a reference condition based on primary or protected forests. We show that the condition across 44 forest types averaged 0.566 in 2000 and increased to 0.585 in 2018. Forest productivity and connectivity are comparable to levels observed in undisturbed or least disturbed forests. One third of the forest area was subject to declining condition, signalled by a reduction in soil organic carbon, tree cover density and species richness of threatened birds. Our findings suggest that forest ecosystems will need further restoration, improvements in management and an extended period of recovery to approach natural conditions.
Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome
Authors: Wang Hongyang , Bienz Mariann , Yan Xiao-Xue , Xu Wenqing 
Published: June 2023

AbstractThe Wnt enhanceosome is responsible for transactivation of Wnt-responsive genes and a promising therapeutic target for treatment of numerous cancers with Adenomatous Polyposis Coli (APC) or β-catenin mutations. How the Wnt enhanceosome is assembled remains poorly understood. Here we show that B-cell lymphoma 9 protein (BCL9), Pygopus (Pygo), LIM domain-binding protein 1 (LDB1) and single-stranded DNA-binding protein (SSBP) form a stable core complex within the Wnt enhanceosome. Their mutual interactions rely on a highly conserved N-terminal asparagine proline phenylalanine (NPF) motif of Pygo, through which the BCL9-Pygo complex binds to the LDB-SSBP core complex. Our crystal structure of a ternary complex comprising the N-terminus of human Pygo2, LDB1 and SSBP2 reveals a single LDB1-SSBP2 complex binding simultaneously to two Pygo2 molecules via their NPF motifs. These interactions critically depend on the NPF motifs which bind to a deep groove formed between LDB1 and SSBP2, potentially constituting a binding site for drugs blocking Wnt/β-catenin signaling. Analysis of human cell lines lacking LDB or Pygo supports the functional relevance of the Pygo-LDB1-SSBP2 interaction for Wnt/β-catenin-dependent transcription.