bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study protocol for testing an innovative gait training program in immersive virtual reality for healthy older adults
Authors: Delbes Lisa , Mascret Nicolas , Goulon Cédric , Montagne Gilles 
Published: May 2023

Abstract
Background Impaired gait adaptability is one of the major causes of falls among older adults by producing inappropriate gait adjustments in cluttered environments. Training programs designed to improve gait adaptability behavior in a systemic approach may prevent falls in older adults. Recently, the technology of virtual reality has appeared as a relevant gait training tool based on its training implementation potential. The present study was designed to compare the effectiveness of a virtual reality gait training program (VR group) for improving gait adaptability behavior and thus, reducing the risk of falls relative to a conventional training program of Nordic walkway (NW group). We hypothesize that the virtual reality gait training program will lead to greater gait adaptability improvements.Methods The protocol describes a randomized controlled trial with pre-tests, post-tests, retention tests and follow-up. Forty healthy independent living community dweller participants (65–80 years) will be allocated, after a general medical examination, to the VR or the NW group for a training program of six weeks. Primary outcome related to gait adaptability capacities and acceptance of the virtual reality device will be assessed in pre- and post-intervention and one month after the completion of the training program (retention). A follow-up will be done during the 12 months after the completion of the gait training program.Discussion This study will demonstrate the relative relevance of a gait training program in virtual reality versus a conventional one for improving gait adaptability behavior in healthy older adults and thus preventing falls.Trial registration CERSTAPS (IRB00012476-2022-14-10-200, 14 October 2022)
Timeliness and completeness of monthly disease surveillance data reporting, Uganda, 2020–2021
Authors: Zavuga Robert , Migisha Richard , Gonahasa Doreen , Kadobera Daniel , Kwesiga Benon , Okello Peter Edward , Bulage Lilian , Aceng Freda Loy , Kayiwa Joshua , Makumbi Issa , Ario Alex Riolexus 
Published: May 2023

Abstract
Background Timely and complete reporting of routine public health information about diseases and public health events are important aspects of a robust surveillance system. Although data on the completeness and timeliness of monthly surveillance data are collected in the District Health Information System-2 (DHIS2), they have not been routinely analysed. For this study, we assessed completeness and timeliness of monthly outpatient department (OPD) data from January 2020 to December 2022.Methods We used DHIS2 data from all 15 regions and 146 districts of Uganda from January 2020─December 2021. Completeness was defined as the number of submitted reports divided by the number of expected reports from the same health facility, district, and region. Timeliness was defined as the number of reports submitted by the deadline (15th day of the following month) divided by reports received. Health facilities, districts, and regions with completeness or timeliness < 80% were regarded as having submitted incomplete or untimely reports.Results Overall, there was good general performance with the median completeness of OPD reports being high in 2020 (99.5%; IQR 97.8–100%) and 2021 (100%; IQR 98.7–100%), as was the median timeliness (2020; 82.8%, IQR 74.6–91.8%; 2021, 94.9%, IQR 86.5–99.1%). In terms of regions, no region reported below the 80% OPD completeness target; Kampala Region was the only region that consistently failed to reach ≥ 80% OPD timeliness (2020: 44%; 2021: 65%). Nakasongola was the only district that consistently performed poorly in the submission of timely reports in both years (2020:54.4%, 2021:58.3%). National referral hospitals consistently failed to meet the timeliness target in both years (2020:47.2%; 2021:74.1).Conclusion There was an overall good performance in the submission of complete and timely monthly OPD reports in both 2020 and 2021 in the DHIS2 across most districts and regions in Uganda. There is a need to strengthen the good reporting practices exhibited and offer support to regions, districts, and health facilities with timeliness challenges.
A Chromosome scale assembly of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), genome
Authors: Perera Omaththage P. , Saha Surya , Glover James , Parys Katherine A. , Allen K. Clint , Grozeva Snejana , Kurtz Ryan , Reddy Gadi V. P. , Johnston J. Spencer , Daly Mark , Swale Thomas 
Published: May 2023

Abstract
Objective: The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), is a pest damaging many cultivated crops in North America. Although partial transcriptome data are available for this pest, a genome assembly was not available for this species. The aim of this was to assemble a high-quality chromosome-length genome of TPB to develop the genetic resources to provide foundation required for advancing research on this species.
Results:
The initial genome of TPB assembled with paired-end nucleotide sequences generated with Illumina technology was scaffolded with Illumina HiseqX reads generated from a proximity ligated (HiC) library to obtain a high-quality genome assembly. The final assembly contained 3968 scaffolds longer than 1 kbp to yield a genome of 599.97 Mbp. The N50 of the TPB genome assembly was 35.64 Mbp and 98.68% of the genome was assembled into 17 scaffolds larger than 1 Mbp. This megabase scaffold number is the same as the number of chromosomes observed in karyotyping of this insect. The TPB genome is known to have high repetitive DNA content, and the reduced assembled genome size compared to flowcytometric estimates of approximately 860 Mbp may be due to the collapsed assembly of highly similar regions.
ϕ-sectional curvatures of homogeneous real hypersurfaces in a complex projective space
Authors: Takagi Ren 
Published: May 2023

Abstract
The purpose of this paper is to compute ϕ-sectional curvatures of homogeneous real hypersurfaces in a complex projective space. In particular, we determine the maximum and minimum values of ϕ-sectional curvatures of homogeneous real hypersurfaces of type (C), (D) and (E) in a complex projective space.
Mathematics Subject Classification. Primary 53B25, Secondary 53C40.
Prediction of bladder cancer grade based on biparametric MRI radiomics: comparison with traditional MRI
Authors: Li Longchao , Zhang Jing , Zhe Xia , Tang Min , Zhang Li , Lei Xiaoyan , Zhang Xiaoling 
Published: May 2023

Abstract
Background: To compare biparametric (bp) MRI radiomics signatures and traditional MRI model for the preoperative prediction of bladder cancer (BCa) grade.
Methods: This retrospective study included 255 consecutive patients with pathologically confirmed 113 low-grade and 142 high-grade BCa who underwent preoperative MRI, including T2-weighted imaging (T2WI) and apparent diffusion coefficient (ADC). The traditional MRI nomogram model was developed using univariate and multivariate logistic regression by the mean apparent diffusion coefficient (mADC), vesical imaging reporting and data system (VI-RADS) scoring, tumor size and number of tumors. Volumes of interest were manually drawn on T2WI and ADC maps by two radiologists. Using ANOVA, correlation and LASSO methods to select features. Then, a logistic regression (LR) classifier was used to develop the radiomics signatures in the training set and assessed in the validation set. Receiver operating characteristic (ROC) analysis was used to compare the diagnostic abilities of the radiomics and traditional MRI models by the DeLong test. Finally, decision curve analysis (DCA) was performed by estimating the clinical usefulness of the two models in both the training and validation sets.
Results: The areas under the ROC curves (AUCs) of the traditional MRI model were 0.841 in the training cohort and 0.806 in the validation cohort. The AUCs of the three groups of radiomics model [ADC, T2WI, bp-MRI (ADC and T2WI)]-based logistic regression analysis algorithms were 0.888, 0.875 and 0.899 in the training cohort and 0.863, 0.805 and 0.867 in the validation cohort, respectively. The combined radiomics model achieved higher AUCs than the traditional MRI model and was compared using the DeLong test (P = 0.026 and 0.023 in the training and validation cohorts, respectively). DCA indicated that the radiomics model had higher net benefits than the traditional MRI model.
Conclusions: The bp-MRI radiomics model may be helpful for distinguishing high-grade and low-grade BCa and outperformed the traditional MRI model. Multicenter validation is needed to acquire high-level evidence for its clinical application.
Rating of Perceived Exertion, a novel method for evaluating maximum bench press performance in inexperienced males
Authors: Hosseini Javid shah , Fashi Mohammad , zandian Hamed 
Published: May 2023

Abstract
Purpose:
The use of standard strength tests as a means of assessing maximum strength is extremely important when resistance training is planned on an individual basis. This study aimed to estimate one repetition maximum (1RM) of healthy males based on the Rating of Perceived Exertion in chest press movement and to provide a special predictive equation.
Methods:
70 healthy males (24.93±0.64 years; BMI: 25.04 ± 0.35 kg/m2) with no previous experience of resistance training, performed one repetition of chest press with eyes close and then chose a number to determine the intensity by Rating of Perceived Exertion scale of 1-10 (CR1-10 scale). The intensity of this repetition was randomly selected based on the percentages of 1RM: 60%, 65%, 70%, 75%, 80%, 85%, 90% and 95%. A special prediction equation was provided based on a mathematical model. Moreover, the Brzycki equation and standard protocol was used to verify the validity of the RPE equation.
Results:
Brzycki equations and standard protocol did not differ significantly with RPE-equation in predicting 1 RM (p>0.05). Based on the Brzycki equation and standard protocol, a 1RM attempt for inexperienced males resulted in 73.99±7.26 kg and 77.50±10.37 respectively, while the RPE equation produced 77.85 ± 8.59 kg. There were significant results for linear regression (p=0.001). Pearson correlation coefficients between Brzycki and standard protocol with predicted 1-RM were 0.961 and 0.962, respectively.
Conclusion:
The new method of 1RM based on RPE performed well in 1RM performance in inexperienced individuals' males, and it appeared safe, accurate, and time-effective.
Physiological response of potato leaves to uniconazole under drought stress at tuber expansion period
Authors: Ding Kaixin , Shan Ying , wang lichun , Tian Guokui , Li Fengyun , Wang Haiyan , Pang Ze , Pan Yang , Jiang Hui 
Published: May 2023

Abstract
Studying the mechanism of drought stress in potato leaves at tuber expansion period by exogenous uniconazole revealed new insights into potato stress physiology. Therefore, a completely random pot experiments with time gradients of 5, 10 and 15 d of drought treatment. were carried out with uniconazole (40 mg L− 1) with respect to photosynthesis, active oxygen metabolism, antioxidant enzyme activity and the ascorbate-glutathione (AsA-GSH) cycle on varieties kexin No 1 (drought-tolerant, KX 1) and Atlantic (drought-sensitive, Atlantic). The results showed that uniconazole can effectively alleviate the degradation of chlorophyll under drought stress, Compared with CK, drought significantly inhibited the Pn, Tr, Gs and Ci of the leaves of the two potato varieties. Exogenous uniconazole effectively alleviated the inhibition of drought on the photosynthetic parameters of potato leaves, compared with D treatment, uniconazole reduced malondialdehyde (MDA), the rate of superoxide radical (O2−) and hydrogen peroxide (H2O2) content in leaves of KX 1 and Atlantic, and increased the activity of antioxidant enzymes in antioxidant defense system to remove excessive accumulation of reactive oxygen species (ROS) and reduce membrane lipid peroxidation, effectively alleviated the loss of yield factors caused by drought stress, and the drought-tolerant variety KX 1 was less damaged by drought than the drought-sensitive variety Atlantic, in addition, under drought stress, the antioxidant enzyme activity and non-enzymatic antioxidant content of the two varieties increased to adapt to the stress response caused by drought stress, D + S treatment could further increase the contents of osmotic adjustment substances (soluble protein and proline) and AsA-GSH cycle products and substrates, including ascorbic acid (AsA), dehydroascorbate (DHA), glutathione (GSH) and oxidized glutathione (GSSG), and increase the ratio of AsA/DHA and GSH/GSSG under drought stress in two potato varieties, so as to improve the level of reducing power and antioxidant capacity in leaves, thus reducing the damage of reactive oxygen species to cell membrane. The drought-tolerant variety KX 1 recovered better than the drought-sensitive variety Atlantic. These results provided a reference for the mechanism of drought resistance in potato and the effect of uniconazole on alleviating drought stress.
Dendrochronological studies of Pinus roxburghii and Pinus wallichiana from Ghora Galli, Murree
Authors: Ullah nadeem , Tayab Muhammad , Rehman Shafiq , Ghumman Muneeb Akram , Waheed Muhammad 
Published: May 2023

Abstract
The present study described the dendrochronological studies of Pinus roxburghii and Pinus wallichiana growing in Ghora Galli, Murree. Study area was subdivided into roadside and away from roadside. It was found that along road side the maximum diameter, age, growth rate and height of Pinus roxburghii were 71 inches, 304 age, 0.277 and 97 feet respectively. Similarly maximum diameter, age, growth rate and height of Pinus roxburghii growing away from road side were 93.5 inches, 328 years, 0.358 inches/year and 99 feet height respectively. Maximum DBH, age, growth rate and height of Pinus wallichiana growing along the roadside was 63 inches, 223 years, 0.392 inches/year and 83 feet height respectively. While, studying the trees of Pinus wallichiana growing away from road side it was found that the maximum DBH, age, growth rate and height were 56.5 inches, 158 years, 0.437 years/inches and 62 respectively. It was also found that growth rate of trees growing away from the road was greater than those growing along the road. A significant correlation between growth rate and height was found While studying the correlation between height and growth rate, it was found that there was no any significant correlation. In vegetation studies, it was found that dominant trees were from Pinaceae family. Shrubs were with three families while herbs contained eleven families and ferns were found with only two families. Maximum IVI from trees was of Pinus wallichiana with 11.07 belonging to Pinaceae family and 7.73 for Hedera nepalensis belonging to Araliaceae family. Similarly, 4.27 was maximum IVI for Artemisia vulgaris of Asteraceae family and 5.44 from ferns the maximum IVI of Adiantum capillus belonging to Adiantaceae family.
Proteome profiling of Campylobacter jejuni 81-176 at 37°C and 42°C by label-free mass spectrometry
Authors: Dreyer Annika , Masanta Wycliffe O. , Lugert Raimond , Bohne Wolfgang , Groß Uwe , Leha Andreas , Dakna Mohammed , Lenz Christof , Zautner Andreas E. 
Published: May 2023

Abstract
Background
The main natural reservoir for Campylobacter jejuni is the intestinal tract of birds, especially in the context of human nutrition chickens and turkeys play the main role. There, C. jejuni multiplies optimally at 42°C, the avian body temperature, while after infection of humans by peroral intake in the human intestinal tract only 37°C prevail. Proteome profiling by label-free mass spectrometry (DIA-MS) was performed to examine the processes which enable C. jejuni strain 81-176 to adapt to 37°C in comparison to 42°C. In total, four states were compared with each other: incubation for 12 h at 37°C, for 24 h at 37°C, for 12 h at 42°C and 24 h at 42°C.
Results
It was shown that the proteome profile changes not only according to the different incubation temperature but also with the length of the incubation period, i.e. significant differences in protein expression were evident when comparing 37°C and 42°C as well as 12 h and 24 h of incubation. Altogether, the expression of 957 proteins was quantifiable. Out of these, 37.1% - 47.3% were considered to be significantly differentially expressed, that means these proteins showed at least a 1.5-fold change in either direction (i.e log2 FC ≥ 0.585 or log2 FC ≤ -0.585) and an FDR-adjusted p-value of less than 0.05. The significantly differentially expressed proteins could be arranged in 4 different clusters and 16 functional catogories.
Conclusions
The C. jejuni proteome at 42°C is better adopted to high replication rates than that at 37°C, which was in particular indicated by the up-expression of proteins belonging to the functional categories "replication", "DNA synthesis and repair factors", "lipid and carbohydrate biosynthesis" and "vitamin synthesis, metabolism, cofactor biosynthesis". The relative up-expression of proteins with chaperone function at 37°C in comparison to 42°C after 12 h incubation indicates a temporary lower-temperature adaptation response. Additionally the up-expression of factors for DNA uptake at 37°C compared to 42°C indicate a higher competence for the acquisition of extraneous DNA at human body temperature.
Evaluating safety risks of whole-body cryotherapy/cryostimulation (WBC): a scoping review from an international consortium
Authors: Legrand Fabien D. , Dugué Benoît , Costello Joe , Bleakley Chris , Miller Elzbieta , Broatch James R. , Polidori Guillaume , Lubkowska Anna , Louis Julien , Lombardi Giovanni , Bieuzen François , Capodaglio Paolo 
Published: May 2023

Abstract
Over the two last decades, whole-body cryotherapy/cryostimulation (WBC) has emerged as an exciting non-pharmacological treatment influencing inflammatory events at a cellular and physiological level, which can result in improved sleep quality, faster neuromuscular recovery after high-intensity exercise, and chronic pain relief for patients suffering different types of diseases (fibromyalgia, rheumatism, arthritis). Some evidence even suggests that WBC has benefits on mental health (depression, anxiety disorders) and cognitive functions in both adults and older adults, due to increased circulating BDNF levels. Recently, some safety concerns have been expressed by influential public health authorities (e.g., FDA, INSERM) based on reports from patients who developed adverse events upon or following WBC treatment. However, part of the data used to support these claims involved individuals whose entire body (except head) was exposed to extreme cold vaporized liquid nitrogen while standing in a narrow bathtub. Such a procedure is known as partial-body cryotherapy (PBC), and is often erroneously mistaken to be whole-body cryotherapy. Although having similarities in terms of naming and pursued aims, these two approaches are fundamentally different. The present article reviews the available literature on the main safety concerns associated with the use of true whole-body cryotherapy. English- and French-language reports of empirical studies including case reports, case series, and randomized controlled trials (RCTs) were identified through searches of PubMed, Scopus, Cochrane, and Web of Science electronic databases. Five case reports and two RCTs were included for a total of 16 documented adverse events (AEs). A critical in-depth evaluation of these AEs (type, severity, context of onset, participant’s medical background, follow-up) is proposed and used to illustrate that WBC-related safety risks are within acceptable limits and can be proactively prevented by adhering to existing recommendations, contraindications, and commonsense guidelines.
Identifying Opportunities for Prevention of Adverse Outcomes Following Female Genital Fistula Repair: Protocol for a Mixed-Methods Study in Uganda
Authors: Ayadi Alison M El , Obore Susan , Kirya Fred , Miller Suellen , Korn Abner , Nalubwama Hadija , Neuhaus John , Getahun Monica , Eyul Patrick , Twine Robert , Andrew Erin V. W. , Barageine Justus K. 
Published: May 2023

Abstract
Background. Female genital fistula is a traumatic debilitating injury, frequently caused by prolonged obstructed labor, affecting between 500,000–2 million women in lower-resource settings. Vesicovaginal fistula causes urinary incontinence. Other gynecologic, neurologic and orthopedic morbidity may occur during fistula development. Women with fistula are stigmatized; limit engagement in social, economic, or religious activities; and report high psychiatric morbidity. Improved global surgical access has reduced fistula consequences yet post-repair risks impacting quality of life and well-being include fistula repair breakdown or recurrence and ongoing or changing urine leakage or incontinence. Limited evidence on risk factors contributing to adverse outcomes hinders interventions to mitigate adverse events, protecting health and quality of life after surgery. This study seeks to identify predictors and characteristics of post-repair fistula breakdown and recurrence (Aim 1) and post-repair incontinence (Aim 2), and to identify feasible and acceptable intervention strategies (Aim 3).Methods. This mixed-methods study incorporates a prospective cohort study of women with successful vesicovaginal fistula repair at approximately 12 fistula repair centers and affiliated care sites in Uganda (Aims 1–2) followed by qualitative inquiry among key stakeholders (Aim 3). Cohort participants will have a baseline visit at the time of surgery followed by data collection at 2 weeks, 6 weeks, 3 months and quarterly thereafter for 3 years. Primary predictors to be evaluated include patient-related factors, fistula-related factors, fistula repair-related factors, and post-repair behaviors and exposures, collected via structured questionnaire at all data collection points. Clinical exams will be conducted at baseline, 2 weeks post-surgery, and for outcome confirmation at symptom development. Primary outcomes are fistula repair breakdown or fistula recurrence and post-repair incontinence. In-depth interviews will be conducted with cohort participants (n ~ 40) and other key stakeholders (~ 40 including family, peers, community members and clinical/social service providers) to develop feasible and acceptable intervention concepts for adjustment of identified risk factors.Discussion. Participant recruitment is underway. This study is expected to identify key predictors that can directly improve fistula repair and post-repair programs and women’s outcomes, optimizing health and quality of life. Furthermore, our study will create a comprehensive longitudinal dataset capable of supporting broad inquiry into post-fistula repair health.Trial Registration. ClinicalTrials.gov Identifier: NCT05437939