bluuebunny/crossref_metadata_2025_split huggingface dataset:
Auf dem Weg zu einer Formyl‐zu‐Phenyl‐Reaktion: Eine unerwartete photochemische Fulven‐Umlagerung
Authors: Lindner Monika M. , Alachraf M. Wasim , Mitschke Benjamin , Schulze Philipp , Leutzsch Markus , List Benjamin 
Published: June 2023

AbstractUm Aldehyde in Arene zu überführen, haben wir eine Sequenz entworfen, bei der ein Aldehyd zunächst zu einem Fulven umgesetzt wird. Daraufhin folgen photochemische und platinkatalysierte Umlagerungen zu einem Dewar‐Benzol‐Derivat, welches schließlich in das gewünschte Aren isomerisiert. Obwohl theoretische Berechnungen die Plausibilität dieses Weges nahelegen, stellten wir experimentell fest, dass die Fulven‐Bestrahlung zu einer unerwarteten Isomerisierung in ein Spiro[2.4]heptadien führt. Diese ungewöhnliche Photoumlagerung wurde mechanistisch untersucht und ermöglicht den Zugang zu verschiedenen Spiro[2.4]heptadienen mit unterschiedlichen Substituenten.
Infrared Multiphoton Dissociation Enables Top‐Down Characterization of Membrane Protein Complexes and G Protein‐Coupled Receptors
Authors: Lutomski Corinne A. , El‐Baba Tarick J. , Hinkle Joshua D. , Liko Idlir , Bennett Jack L. , Kalmankar Neha V. , Dolan Andrew , Kirschbaum Carla , Greis Kim , Urner Leonhard H. , Kapoor Parth , Yen Hsin‐Yung , Pagel Kevin , Mullen Christopher , Syka John E. P. , Robinson Carol V. 
Published: June 2023

AbstractMembrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires stabilization in detergent micelles that are removed prior to analysis via collisional activation. There is however a practical limit to the amount of energy which can be applied, which often precludes subsequent characterization by top‐down MS. To overcome this barrier, we have applied a modified Orbitrap Eclipse Tribrid mass spectrometer coupled to an infrared laser within a high‐pressure linear ion trap. We show how tuning the intensity and time of incident photons enables liberation of membrane proteins from detergent micelles. Specifically, we relate the ease of micelle removal to the infrared absorption of detergents in both condensed and gas phases. Top‐down MS via infrared multiphoton dissociation (IRMPD), results in good sequence coverage enabling unambiguous identification of membrane proteins and their complexes. By contrasting and comparing the fragmentation patterns of the ammonia channel with two class A GPCRs, we identify successive cleavage of adjacent amino acids within transmembrane domains. Using gas‐phase molecular dynamics simulations, we show that areas prone to fragmentation maintain aspects of protein structure at increasing temperatures. Altogether, we propose a rationale to explain why and where in the protein fragment ions are generated.
Discussion of specifying prior distributions in reliability applications—Applications for Bayesian estimation software design
Authors: Liu Peng 
Published: June 2023

AbstractIt is a great pleasure to have the opportunity to write a discussion on “Specifying Prior Distributions in Reliability Applications” by Tian et al. Appl Stochast Models Bus Ind, (2023). One coauthor of the paper, Dr Meeker, has conducted Bayesian methodology research on reliability data analysis for many years, and I have followed his work on the subject for quite some time. The work by Dr Meeker helped us develop Bayesian estimation products which are both powerful and easy to use. This time, I learned something new as usual. In this discussion, I will focus on the great value of the paper for developing user friendly Bayesian estimation software.
Intense green light emission and thermoluminescence glow curve analysis of Tb3+‐activated CaY2O4 phosphor
Authors: Ram Tirath , Verma Neeraj , Kaur Jagjeet , Misra Abhishek Kumar , Dubey Vikas , Dubey Neha , Vij Ankush , Rao Myla Chimpiri 
Published: June 2023

AbstractHere, the synthesis and luminescence analysis of the Tb3+‐activated phosphor were reported. The CaY2O4 phosphors were synthesized using a modified solid‐state reaction method with a variable doping concentration of Tb3+ ion (0.1–2.5 mol%). As synthesized, the phosphor was characterized using Fourier transform infrared spectroscopy (FTIR) and X‐ray diffraction analysis techniques for the optimized concentration of doping ions. The prepared phosphor showed a cubic structure, and FTIR analysis confirmed functional group analysis. It was discovered that the intensity of 1.5 mol% was higher than at other concentrations after the photoluminescence (PL) excitation and emission spectra were recorded for different concentrations of doping ions. The excitation was monitored at 542 nm, and the emission was monitored at 237 nm. At 237 nm excitation, the emission peaks were found at 620 nm (5D4→7F3), 582 nm (5D4→7F4), 542 nm (5D4→7F5), and 484 nm (5D4→7F6). The 1931 CIE (x, y) chromaticity coordinates showed the distribution of the spectral region calculated from the PL emission spectra. The values of (x = 0.34 and y = 0.60) were very close to dark green emission. Therefore, the produced phosphor would be very useful for light‐emitting diode (green component) applications. Thermoluminescence glow curve analysis for various concentrations of doping ions and various ultraviolet (UV) exposure times was carried out, and a single broad peak was found at 252°C. The computerized glow curve deconvolution method was used to obtain the related kinetic parameters. The prepared phosphor exhibited an excellent response to UV dose and could be useful for UV ray dosimetry.
Reusable flexible poly(vinyl alcohol)/chitosan‐based polymer carbon dots composite film for acid blue 93 dye adsorption
Authors: Yu Shujuan , Lu Shiyan , Zheng Guangjian 
Published: June 2023

AbstractThe design and synthesis of water‐insoluble chitosan‐based polymer carbon dots [P(CS‐g‐CA)CDs] are described. A polyvinyl alcohol/chitosan‐based polymer carbon dot [PVA/P(CS‐g‐CA)CDs] composite film was prepared using a simple casting method to be used in dye adsorption. The composite film was characterized using FT‐IR, XPS, transparency, contact angle, and mechanical properties tests, which showed the successful incorporation of P(CS‐g‐CA)CDs into the film and also revealed that hydrogen bonding improved the mechanical properties of the PVA film. Furthermore, the composite film displayed substantially enhanced hydrophobicity, making it suitable for use in aqueous environments. In addition, the composite film exhibited stable adsorption of acid blue 93 (AB93) at pH 2–9, with an enhanced adsorption capacity of 433.24 mg/g. The adsorption obeyed Langmuir law with an efficiency of more than 89% even after five cycles. Therefore, the PVA/P(CS‐g‐CA)CDs film is a promising material for the treatment of organic dye‐polluted wastewater.
Sustainability partnership as a moderator in the relationship between business sustainability and firm competitiveness
Authors: Saunila Minna , Ukko Juhani , Kinnunen Jorma 
Published: June 2023

AbstractThis paper empirically examines the relationship between business sustainability and firm competitiveness and the role of sustainability partnerships in the relationship between business sustainability and firm competitiveness. By classifying two dimensions (environmental sustainability and social sustainability) that are needed to realize business sustainability, this study suggests that a sustainability partnership serves as a moderator in the relation between business sustainability and firm competitiveness. Three developed hypotheses were tested using regression analysis to find the relationship between business sustainability, sustainability partnership, and firm competitiveness. Using a structured survey questionnaire, the data were collected from 135 firms that operate in construction industries in Finland. The findings suggest that investing in environmental sustainability will directly improve firm competitiveness, but investing in social sustainability will not. In addition, sustainability partnerships serve as a promoter in the relationship between social sustainability and firm competitiveness but inhibit the relationship between environmental sustainability and firm competitiveness.
Identification of predictors of long‐term survival and prognostic outcomes in thymic squamous cell carcinoma: A real‐world study
Authors: Zhou Meng‐Xin , Chen Ye‐Ye , Huang Cheng , Liu Lei , Wang Gui‐Ge , Zhang Jia‐Qi , Bai Wen‐Liang , Zhao Ke , Li Shan‐Qing 
Published: June 2023

AbstractBackgroundAlthough thymic squamous cell carcinoma (TSCC) is among the most prevalent forms of thymic carcinoma, there are relatively few studies on this tumor type, and its staging, optimal treatment strategies, and relevant prognostic factors remain controversial.MethodsThe present study analyzed 79 patients diagnosed with TSCC between January 2008 and January 2021. Kaplan–Meier curves and Cox univariate and multivariate regression analyses were used to explore factors associated with overall survival (OS) and progression‐free survival (PFS) in the overall patient cohort and patient subgroups stratified according to the TNM stage. Time‐dependent receiver operating characteristic (ROC) analyses were used to compare the TNM and Masaoka systems as predictors of patient prognosis.ResultsThe 5‐ and 10‐year OS rates in this study were 65.5% and 49.4%, respectively, with corresponding 5‐ and 10‐year PFS rates of 52.3% and 37.9%. Survival outcomes were better for patients with early‐stage disease (p < 0.001) and patients that underwent surgical treatment (p < 0.001). Neither extent of resection (p = 0.820) nor the surgical approach (p = 0.444) influenced patient survival. In individuals with advanced disease, all forms of adjuvant therapy including radiotherapy (p = 0.021), chemotherapy (p = 0.035), and chemoradiation (p = 0.01) significantly improved patient PFS, but only adjuvant chemoradiotherapy improved patient OS (p = 0.035). When predicting the patient survival outcomes, the TNM system was slightly superior to the Masaoka system (area under the ROC curve [AUC] at 5 years: OS, 0.742 vs. 0.723; PFS, 0.846 vs. 0.816).ConclusionTSCC is an orphan malignancy with a poor prognosis. TNM staging may be superior to Masaoka staging as a predictor of TSCC patient prognosis. Surgery is the mainstay of TSCC treatment. Video‐assisted thoracoscopy (VATS) should be considered for selected patients. Multimodal therapy was associated with excellent results for patients with advanced TNM stage, particularly when surgery was accompanied by adjuvant chemoradiation.
Immunohistochemistry and oxygen saturation endoscopic imaging reveal hypoxia in submucosal invasive esophageal squamous cell carcinoma
Authors: Minakata Nobuhisa , Sakashita Shingo , Wakabayashi Masashi , Nakamura Yuka , Sunakawa Hironori , Yoda Yusuke , Ishii Genichiro , Yano Tomonori 
Published: June 2023

AbstractBackgroundHypoxic microenvironment is prominent in advanced esophageal squamous cell carcinoma (ESCC). However, it is unclear whether ESCC becomes hypoxic when it remains in the mucosal layer or as it invades the submucosal layer. We aimed to investigate whether intramucosal (Tis‐T1a) or submucosal invasive (T1b) ESCC becomes hypoxic using endoscopic submucosal dissection samples.MethodsWe evaluated the expression of hypoxia markers including hypoxia inducible factor 1α (HIF‐1α), carbonic anhydrase IX (CAIX), and glucose transporter 1 (GLUT1) by H‐score and vessel density by microvessel count (MVC) and microvessel density (MVD) for CD31 and α‐smooth muscle actin (α‐SMA) with immunohistochemical staining (n = 109). Further, we quantified oxygen saturation (StO2) with oxygen saturation endoscopic imaging (OXEI) (n = 16) and compared them to non‐neoplasia controls, Tis‐T1a, and T1b.ResultsIn Tis‐T1a, cccIX (13.0 vs. 0.290, p < 0.001) and GLUT1 (199 vs. 37.6, p < 0.001) were significantly increased. Similarly, median MVC (22.7/mm2 vs. 14.2/mm2, p < 0.001) and MVD (0.991% vs. 0.478%, p < 0.001) were markedly augmented. Additionally, in T1b, the mean expression of HIF‐1α (16.0 vs. 4.95, p < 0.001), CAIX (15.7 vs. 0.290, p < 0.001), and GLUT1 (177 vs. 37.6, p < 0.001) were significantly heightened, and median MVC (24.8/mm2 vs. 14.2/mm2, p < 0.001) and MVD (1.51% vs. 0.478%, p < 0.001) were markedly higher. Furthermore, OXEI revealed that median StO2 was significantly lower in T1b than in non‐neoplasia (54% vs. 61.5%, p = 0.00131) and tended to be lower in T1b than in Tis‐T1a (54% vs. 62%, p = 0.0606).ConclusionThese results suggest that ESCC becomes hypoxic even at an early stage, and is especially prominent in T1b.
YAP/STAT3 inhibited CD8+ T cells activity in the breast cancer immune microenvironment by inducing M2 polarization of tumor‐associated macrophages
Authors: Wang Chen , Shen Ningning , Guo Qingling , Tan Xiaohua , He Shaozhong 
Published: June 2023

AbstractBackgroundBreast cancer (BC) is the leading cause of cancer‐related death among women. One of the hallmarks of cancer is sustained angiogenesis. YAP/STAT3 may promote angiogenesis and driving BC progression. This study aimed to investigate how YAP/STAT3 affects the immune microenvironment in BC and understand the underlying mechanism.MethodsTo establish a tumor‐associated macrophages (TAMs) model, macrophages were cultured in the 4T1 cell culture medium. A BC mouse model was created by injecting 4T1 cells. The expression of YAP, STAT3, p‐STAT3, VEGF, VEGFR‐2, and PD‐L1 was analyzed using immunofluorescence, western blotting, and quantitative real‐time PCR. Flow cytometry was used to identify M1 and M2 macrophages, CD4+ T, CD8+ T, and Treg cells. Levels of iNOS, IL‐12, IL‐10, TGF‐β, Arg‐1, and CCL‐22 were measured using enzyme‐linked immunosorbent assay. Co‐IP was used to verify whether YAP binds to STAT3. Hematoxylin–eosin staining was used to observe tumor morphology. Cell counting kit‐8 was selected to detect T‐cell proliferation.ResultsYAP, STAT3, P‐STAT3, VEGF, VEGFR‐2, and PD‐L1 were highly expressed in BC tissues. The M2/M1 macrophages ratio increased in the TAMs group compared with the control group. Inhibiting of YAP and STAT3 decreased the M2/M1 macrophages ratio. YAP was found to bind with STAT3. T‐cell proliferation was enhanced after YAP inhibition, and overexpression of STAT3 reversed the regulation of YAP on T‐cell proliferation. In animal studies, inhibiting YAP inhibited tumor weight and volume development. After YAP inhibition, inflammatory infiltration, M2/M1 macrophage ratio, and Treg cell ratio declined, while CD8+ and CD4+ T‐cell ratio increased.ConclusionIn conclusion, this study suggested inhibition of YAP/STAT3 reversed M2 polarization of TAMs and suppressed CD8+ T‐cell activity in the BC immune microenvironment. These findings open up new avenues for the development of innovative therapies in the treatment of BC.
Clinical and metabolomic characterization of Brivanib‐Induced hypertension in metastatic colorectal cancer
Authors: Rattner Jodi I. , Kopciuk Karen A. , Vogel Hans J. , Tang Patricia A. , Shapiro Jeremy D. , Tu Dongsheng , Jonker Derek J. , Siu Lillian L. , O'Callaghan Chris J. , Bathe Oliver F. 
Published: June 2023

AbstractBackgroundTrials of tyrosine kinase inhibitors (TKI) have not demonstrated dramatic benefits in advanced colorectal cancer (CRC), and this may be a function of poor patient selection. TKI‐induced hypertension is reportedly a surrogate marker for treatment benefit for some tumor types. Our objective was to determine whether hypertension was associated with benefit in the context of CRC treatment, and also to gain insight on the pathogenesis of TKI‐induced hypertension by monitoring associated changes in the circulating metabolome.Patients and MethodsClinical data were acquired from clinical trial patients with metastatic CRC randomized to cetuximab ± the TKI brivanib (N = 750). Outcomes were evaluated as a function of treatment‐induced hypertension. For metabolomic studies, plasma samples were taken at baseline, as well as at 1, 4, and 12 weeks after treatment initiation. Samples were submitted to gas chromatography–mass spectrometry to identify treatment‐related metabolomic changes associated with TKI‐induced hypertension, compared to pre‐treatment baseline. A model based on changes in metabolite concentrations was generated using orthogonal partial least squares discriminant analysis (OPLS‐DA).ResultsIn the brivanib treated group, 95 patients had treatment‐related hypertension within 12 weeks of initiating treatment. TKI‐induced hypertension was not associated with a significantly higher response rate, nor was it associated with improved progression‐free or overall survival. In metabolomic studies, 386 metabolites were identified. There were 29 metabolites that changed with treatment and distinguished patients with and without TKI‐induced hypertension. The OPLS‐DA model for brivanib‐induced hypertension was significant and robust (R2Y score = 0.89, Q2Y score = 0.70, CV‐ANOVA = 2.01 e‐7). Notable metabolomic features previously reported in pre‐eclampsia and associated with vasoconstriction were found.ConclusionTKI‐induced hypertension was not associated with clinical benefit in metastatic CRC. We have identified changes in the metabolome that are associated with the development of worsening brivanib‐induced hypertension that may be useful in future efforts of characterizing this toxicity.
The prognostic and predictive roles of plasma C‐reactive protein and PD‐L1 in non‐small cell lung cancer
Authors: Kuusisalo Saara , Tikkanen Antti , Lappi‐Blanco Elisa , Väisänen Timo , Knuuttila Aija , Tiainen Satu , Ahvonen Jarkko , Iivanainen Sanna , Koivunen Jussi P. 
Published: June 2023

AbstractBackgroundAnti‐PD‐(L)1 agents have revolutionized the treatment paradigms of non‐small cell lung cancer (NSCLC), while predictive biomarkers are limited. It has been previously shown that systemic inflammation, indicated by elevated C‐reactive protein (CRP) level, is associated with a poor prognosis in anti‐PD‐(L)1 treated. The aim of the study was to analyze the prognostic and predictive value of CRP in addition to traditional prognostic and predictive markers and tumor PD‐L1 score.MethodsWe identified all NSCLC patients (n = 329) who had undergone PD‐L1 tumor proportion score (TPS) analysis at Oulu University Hospital 2015–22. CRP levels, treatment history, immune checkpoint inhibitor (ICI) therapy details, and survival were collected. The patients were categorized based on CRP levels (≤10 vs. >10) and PD‐L1 TPS scores (<50 vs. ≥50).ResultsIn the whole cohort (n = 329), CRP level of ≤10 mg/L was associated with improved survival in univariate (HR 0.30, Cl 95% 0.22–0.41) and multivariate analyzes (HR 0.44, CI 95% 0.28–0.68). With ICI treated (n = 70), both CRP of ≤10 and PD‐L1 TPS of ≥50 were associated with improved progression‐free survival (PFS) in univariate (HR 0.51, CI 95% 0.27–0.96; HR 0.54, CI 95% 0.28–1.02) and multivariate (HR 0.48, CI 95% 0.26–0.90; HR 0.50, CI 95% 0.26–0.95) analyzes. The combination (PD‐L1 TPS ≥50 and CRP >10) carried a high negative predictive value with a median PFS of 4.11 months (CI 95% 0.00–9.63), which was similar to patients with low PD‐L1 (4.11 months, CI 95% 2.61–5.60).ConclusionsAdding plasma CRP levels to PD‐L1 TPS significantly increased the predictive value of sole PD‐L1. Furthermore, patients with high CRP beard little benefit from anti‐PD‐(L)1 therapies independent of PD‐L1 score. The study highlights the combined evaluation of plasma CRP and PD‐L1 TPS as a negative predictive marker for ICI therapies.
Design, Synthesis, Biological Evaluation and Molecular Docking Studies of Quercetin‐Linker‐H2S Donor Conjugates for the Treatment of Diabetes and Wound Healing**
Authors: He Shibo , Liu Jian , Hu Lifei , Zhan Yifeng , Tong Hang , Zhu Hongda , Guo Huiling , Sun Hongmei , Liu Mingxing 
Published: June 2023

AbstractBased on the use of quercetin for treating diabetes and H2S for promoting wound healing, a series of three quercetin‐linker‐H2S donor conjugates was designed, synthesized and characterized by 1H‐NMR, 13C‐NMR and MS. Meanwhile, in vitro evaluation of these compounds was also researched by IR‐HepG2 treatment experiment, MTT assay, scratch test and tubule formation experiment. The three compounds could be used to treat insulin resistance induced by high glucose and promote the proliferation of human umbilical vein endothelial cells, wound healing, and the formation of tubules in vitro under a high‐glucose environment. Our results illustrate that these compounds could be used to treat diabetes and promote wound healing at the same time. Furthermore, molecular docking study results of the compounds were consistent with the evaluated biological activity. In vivo research of compounds is underway.
Heterotaxy pattern associated with sinus node dysfunction in an adult: A case report
Authors: Shah Naman , Acharya Sankalp , Tripathi Apoorva , Bisht Himanshi , Shah Maitri , Pareek Aayushi , Gera Asmita , Shrestha Abhigan Babu , Jaiswal Vikash 
Published: June 2023

Key Clinical MessageA 26‐year‐old male patient admitted to the hospital ward with experience of repetitive syncopes for a year. The patient was diagnosed with sick sinus syndrome. The aim of this clinical report is to highlight the variability of anatomical findings associated with polysplenia pattern.AbstractThis case report presents a 26‐year‐old male patient who presented to the medical ward with a complaint of repeating blackouts for a year. The patient was then diagnosed with sick sinus syndrome, and further investigations revealed left isomerism, polysplenia, and no congenital heart defects. Holter monitoring, ultrasonography, electrocardiography, and computed tomography were used to confirm the diagnosis. The patient underwent DDDR pacemaker implantation for the treatment of SA node dysfunction. The report highlights the variability of anatomical findings associated with polysplenia pattern and the various types of heartbeat disruptions that may occur in the atrial appendages of the left side isomerism.
Renal arteriovenous fistula discovered ~2 years after renal biopsy: A case report
Authors: Oyama Maki , Tamura Hiroshi , Hidaka Yuko , Furuie Keishiro , Kuraoka Shohei 
Published: June 2023

Key Clinical MessageAlthough percutaneous renal biopsy is considered safe, this invasive procedure has complications such as renal arteriovenous fistula (RAVF). Even if complications such as RAVFs are not observed early after renal biopsy, considering the possibility of delayed renal hemorrhage, follow‐up with ultrasound after renal biopsy even in asymptomatic cases could be important.AbstractAlthough percutaneous renal biopsy is considered safe, this invasive procedure can lead to complications such as renal arteriovenous fistula (RAVF). RAVF occurs when some arteries and veins communicate in the absence of capillaries in the renal hilum or renal parenchyma. It was previously thought to be relatively rare; however, with advances in imaging diagnostics, it is sometimes found asymptomatically. In addition, renal biopsy is the most common cause of acquired RAVF. In this case, RAVF was discovered 2 years after renal biopsy. Late‐onset RAVF is scarce. This case highlights that even if complications such as RAVFs are not observed early after renal biopsy, considering the possibility of delayed RAVF, follow‐up with ultrasound could be important.