bluuebunny/crossref_metadata_2025_split huggingface dataset:
K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis
Authors: Zhang Xiao-Lan , Chen Xin-Hui , Xu Binwu , Chen Min , Zhu Song , Meng Nan , Wang Ji-Zhong , Zhu Huifang , Chen De , Liu Jin-Bao , Yan Guang-Rong 
Published: June 2023

AbstractN6-methyladenosine (m6A) modification plays important roles in bioprocesses and diseases. AlkB homolog 5 (ALKBH5) is one of two m6A demethylases. Here, we reveal that ALKBH5 is acetylated at lysine 235 (K235) by lysine acetyltransferase 8 and deacetylated by histone deacetylase 7. K235 acetylation strengthens the m6A demethylation activity of ALKBH5 by increasing its recognition of m6A on mRNA. RNA-binding protein paraspeckle component 1 (PSCP1) is a regulatory subunit of ALKBH5 and preferentially interacts with K235-acetylated ALKBH5 to recruit and facilitate the recognition of m6A mRNA by ALKBH5, thereby promoting m6A erasure. Mitogenic signals promote ALKBH5 K235 acetylation. K235 acetylation of ALKBH5 is upregulated in cancers and promotes tumorigenesis. Thus, our findings reveal that the m6A demethylation activity of ALKBH5 is orchestrated by its K235 acetylation and regulatory subunit PSPC1 and that K235 acetylation is necessary for the m6A demethylase activity and oncogenic roles of ALKBH5.
Subcellular second messenger networks drive distinct repellent-induced axon behaviors
Authors: Baudet Sarah , Zagar Yvrick , Roche Fiona , Gomez-Bravo Claudia , Couvet Sandrine , Bécret Johann , Belle Morgane , Vougny Juliette , Uthayasuthan Sinthuya , Ros Oriol , Nicol Xavier 
Published: June 2023

AbstractSecond messengers, including cAMP, cGMP and Ca2+ are often placed in an integrating position to combine the extracellular cues that orient growing axons in the developing brain. This view suggests that axon repellents share the same set of cellular messenger signals and that axon attractants evoke opposite cAMP, cGMP and Ca2+ changes. Investigating the confinement of these second messengers in cellular nanodomains, we instead demonstrate that two repellent cues, ephrin-A5 and Slit1, induce spatially segregated signals. These guidance molecules activate subcellular-specific second messenger crosstalk, each signaling network controlling distinct axonal morphology changes in vitro and pathfinding decisions in vivo.
Ensemble of CMIP6 derived reference and potential evapotranspiration with radiative and advective components
Authors: Bjarke Nels , Barsugli Joseph , Livneh Ben 
Published: June 2023

AbstractAssessing changes in future aridity requires an understanding of variations in the atmospheric demand for water. Such assessments are often driven by estimations of potential evapotranspiration (ETP) and/or reference evapotranspiration (ET0), yet no comprehensive and validated estimate of these climate metrics exists to date from the Coupled Model Intercomparison Project 6 (CMIP6). Here we describe the development and validation of a published dataset of global monthly estimates of the Penman-Monteith derived ET0, its advective and radiation components, Priestley-Taylor derived ETP, and vapor pressure deficit from 16 CMIP6 projections and four emissions scenarios. Historical validation of the ensemble of CMIP6 evaporative demand shows general agreement with observationally-derived baselines of ET0 and ETP from the Climate Research Unit (CRU) and ERA5-Land reanalysis products, with GCM biases driven primarily by regional differences in modeled humidity and advective contributions to ET0. Overall, evaporative demand is projected to increase across all emissions scenarios, with the largest increases over polar regions, and with a larger contribution from advection particularly for regions with higher baseline ET0.
Social influence makes outlier opinions in online reviews offer more helpful information
Authors: Yang Kunhao , Fujisaki Itsuki , Ueda Kazuhiro 
Published: June 2023

AbstractIdentifying helpful information from large-scale online reviews has become a core issue in studies on harnessing wisdom-of-crowds. We investigated whether online reviews expressing dissenting opinions (i.e., outlier reviews) can provide helpful information. Using statistical and simulation methods with a large-scale dataset, we found that, compared with other online reviews, outlier reviews were deemed more helpful because they provided more sufficient, neutral, and concise information. To interpret these results, we considered that in collective behaviours, a prevalent social psychological process—conformity (i.e., changing one’s behaviour in response to pressure from others)—pressured reviewers expressing dissenting opinions. This motivated them to provide more convincing evidence (i.e., sufficient, neutral, and concise information). This study offers a simple yet effective approach for eliciting helpful information from many online reviews and deepens the understanding of the mechanism underlying collective online behaviour. Specifically, conformity was considered to cause biases in the collective behaviour of humans; however, this study revealed that conformity can elicit valuable outcomes in collective behaviour.
Change of world-record rankings of shot put and hammer throw due to the effects of Earth rotation and athlete’s height
Authors: Horváth Gábor , Hegedűs Dénes , Slíz-Balogh Judit 
Published: June 2023

AbstractThe differences between the consecutive world-records of shot put and hammer throw tendentiously decrease. Therefore, nowadays it would be worth taking into account the influence of certain environmental factors on the range L, such as the latitude and release azimuth direction. Both factors exert influence on L by the centrifugal acceleration acentrigugal and Coriolis acceleration aCoriolis induced by the Earth’s rotation. The aim of this work is to reveal how the world-record ranking numbers would change, if acentrigugal and aCoriolis as well as the athletes’ height h were taken into account during the validation of the outdoor world records of senior female and male shot-putters and hammer-throwers. Using computer modelling, we reconstruct here the release velocities v of shots and hammers and the normalized muscle work of shot-putters achieved at the 20 best consecutive world records. We determined numerous changes of world-record ranking numbers of shot put and hammer throw due to the influence of acentrigugal, aCoriolis and h. Height h has the largest effect on the range L, acentrigugal has a medium influence, and aCoriolis possesses the least impact on L. The physically most correct way would be to consider the release velocity v of the shot/hammer (easily measurable with an ultrasound/laser Doppler gauge) as the real performance of athletes, because it is practically independent of the environmental factors, and thus is a much better ranking measure of world records than the range.
Deep neural network analysis models for complex random telegraph signals
Authors: Robitaille Marcel , Yang HeeBong , Wang Lu , Deng Bowen , Kim Na Young 
Published: June 2023

AbstractTime-fluctuating signals are ubiquitous and diverse in many physical, chemical, and biological systems, among which random telegraph signals (RTSs) refer to a series of instantaneous switching events between two discrete levels from single-particle movements. A reliable RTS analysis is a crucial prerequisite to identify underlying mechanisms related to device performance and sensitivity. When numerous levels are involved, complex patterns of multilevel RTSs occur and make their quantitative analysis exponentially difficult, hereby systematic approaches are often elusive. In this work, we present a three-step analysis protocol via progressive knowledge-transfer, where the outputs of the early step are passed onto a subsequent step. Especially, to quantify complex RTSs, we resort to three deep neural network architectures whose trained models can process raw temporal data directly. We furthermore demonstrate the model accuracy extensively with a large dataset of different RTS types in terms of additional background noise types and amplitude size. Our protocol offers structured schemes to extract the parameter values of complex RTSs as imperative information with which researchers can draw meaningful and relevant interpretations and inferences of given devices and systems.
Electrochemical sensor based on the synergy between Cucurbit[8]uril and 2D-MoS2 for enhanced melatonin quantification
Authors: Martínez-Moro Rut , del Pozo María , Vázquez Luis , Martín-Gago José A. , Petit-Domínguez María Dolores , Casero Elena , Quintana Carmen 
Published: June 2023

AbstractWe present the development of an electrochemical sensor towards melatonin determination based on the synergistic effect between MoS2nanosheets and cucurbit[8]uril. For the sensor construction cucurbit[8]uril suspensions were prepared in water, and MoS2nanosheets were obtained by liquid exfoliation in ethanol:water. The sensing platform was topographically characterized by Atomic Force Microscopy. Electrochemical Impedance Spectroscopy experiments allowed us to study the charge transfer process during melatonin oxidation. Moreover, stoichiometry of the resulting complex has also been determined. After the optimization of the sensor construction and the experimental variables involved in the Differential Pulse Voltammetric response of melatonin, detection limit of 3.80 × 10−7 M, relative errors minor than 3.8% and relative standard deviation lower than 4.4% were obtained. The proposed sensor has been successfully applied to melatonin determination in pharmaceutical and biological samples as human urine and serum, with very good recoveries ranging from 90 to 102%.
The dynamic functional connectivity fingerprint of high-grade gliomas
Authors: Moretto Manuela , Silvestri Erica , Facchini Silvia , Anglani Mariagiulia , Cecchin Diego , Corbetta Maurizio , Bertoldo Alessandra 
Published: June 2023

AbstractResting state fMRI has been used in many studies to investigate the impact of brain tumours on functional connectivity (FC). However, these studies have so far assumed that FC is stationary, disregarding the fact that the brain fluctuates over dynamic states. Here we utilised resting state fMRI data from 33 patients with high-grade gliomas and 33 healthy controls to examine the dynamic interplay between resting-state networks and to gain insights into the impact of brain tumours on functional dynamics. By employing Hidden Markov Models, we demonstrated that functional dynamics persist even in the presence of a high-grade glioma, and that patients exhibited a global decrease of connections strength, as well as of network segregation. Furthermore, through a multivariate analysis, we demonstrated that patients’ cognitive scores are highly predictive of pathological dynamics, thus supporting our hypothesis that functional dynamics could serve as valuable biomarkers for better understanding the traits of high-grade gliomas.
CT imaging shows specific pancreatic abnormalities in persons with cystic fibrosis related diabetes
Authors: Alexandre-Heymann Laure , Puerto Marie , Martin Clémence , Burnet Espérie , Mosnier-Pudar Helen , Burgel Pierre-Régis , Larger Etienne 
Published: June 2023

AbstractCystic fibrosis related diabetes (CFRD) is observed in 20–50% of adults with cystic fibrosis (CF). Pancreas abnormalities on imaging, e.g. pancreas lipomatosis, are frequent in subjects with CF. We hypothesized that specific abnormalities may characterize patients with CFRD. We performed a retrospective study comparing the computed tomography (CT) of participants with CF with or without diabetes (“CFRD” and “CF control” groups). We classified the pancreas on imaging according to 3 categories: normal, partial lipomatosis and complete lipomatosis of the pancreas. We also assessed the presence or absence of pancreatic calcifications. Forty-one CFRD and 53 CF control participants were included. Only 2% of the patients with CFRD had a normal pancreas, as compared with 30% of the participants from the CF control group (p = 0.0016). Lipomatosis was more frequent in subjects with CFRD and was related to exocrine pancreatic insufficiency (EPI) and to severe CFTR mutations (classes I to III). Nine participants with diabetes (22%) presented with pancreatic calcifications, versus none of the control participants (p = 0.0003). In conclusion, pancreas imaging was almost always abnormal in subjects with CFRD, while it was normal in a third of the CF control subjects. Pancreatic calcifications were specific of subjects with CFRD.
Chain-specificity of laminin α1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding
Authors: Matsunuma Masumi , Kan Ryuji , Yamada Yuji , Hamada Keisuke , Kanagawa Motoi , Nomizu Motoyoshi , Kikkawa Yamato 
Published: June 2023

AbstractLaminins are a family of heterotrimers composed of α-, β-, and γ-chains in the basement membrane. Five α chains contain laminin globular (LG) domain consisting of five tandem modules (LG1-5 modules) at their C-terminus. Each LG45 modules is connected to a compact cloverleaf-shaped structure of LG1-3 through a flexible linker. Although the accumulated studies of the LG45 modules have suggested differences in each α chain regarding the binding of carbohydrate chain and intramolecular interaction, this remains unclear. In this study, to characterize their functions comparatively, we produced recombinant proteins of LG45 modules of human laminin α1-5 chains. Dystroglycan (DG) modified with matriglycan readily bound to the LG45 modules of α1 and α2 chains but not to the other α chains. In contrast, heparin bound to the LG45 modules of the α chains, except for α2. The binding of heparan sulfate/heparin-linked syndecans (SDCs) to LG45 modules was influenced by their core proteins. Furthermore, the α1 and α4LG45 modules bound to SDCs in a pH-dependent manner. A cell adhesion assay showed that HEK293 cells could readily adhere to the LG45 modules of α3-5 chains through a combination of SDCs and integrins. Moreover, α5LG45 modules bound to the E8 fragment, which includes the C-terminus of the laminin coiled-coil (LCC) domain and LG1-3 modules, but α2LG45 modules did not. The results suggested that although α5LG45 modules was fixed within the LG domain, α2LG45 modules was freely placed in the vicinity of LG1–3. Our findings provide information for investigation of the structural and functional diversity of basement membranes.
Plastiglomerates from uncontrolled burning of plastic waste on Indonesian beaches contain high contents of organic pollutants
Authors: Utami Dwi Amanda , Reuning Lars , Schwark Lorenz , Friedrichs Gernot , Dittmer Ludwig , Nurhidayati Ayu Utami , Al Fauzan Ahmad , Cahyarini Sri Yudawati 
Published: June 2023

AbstractThis study reports on plastiglomerate and other new forms of plastic pollution in the tropical marine continent of Indonesia. Twenty-five samples were collected from an island beach in the Java Sea where plastiglomerate, plasticrusts, and pyroplastic were formed by the uncontrolled burning of plastic waste. The most common plastic types were polyethylene and polypropylene (PE/PP), as shown by ATR-FTIR spectroscopy. However, acrylates/polyurethane/varnish (PU) and a copolymer of styrene and acrylonitrile were found as well. This suggests that plastiglomerates can form from a wider variety of plastic polymers than previously reported. FTIR analysis also indicates thermo-oxidative weathering, making the charred plastic more brittle and susceptible to microplastic formation. A subset of the samples was analyzed for associated chemical contaminants. One plastiglomerate with a PU matrix showed high concentrations of phthalates. All samples had high concentrations of polycyclic aromatic hydrocarbons (PAHs), likely due to the burning of the plastic in open fires. The burning leads to a change in the physical and chemical properties of the plastics contained in the plastiglomerates. Plastiglomerate and plastic waste of similar origin are therefore often more weathered and contaminated with organic pollutants than their parent polymers. The highest PAH concentration was found in a plastitar sample. Plastitar is defined as an agglomerate of tar and plastics that adheres to coastal rocks. In contrast, our study documents a more mobile, clastic plastitar type. This clastic plastitar could pose an additional ecological risk because of its mobility. These new types of plastic pollution could be an important vector for chemical contamination of nearby coastal habitats such as coral reefs, seagrass meadows, and mangroves.
Ion mobility mass spectrometry for the study of mycobacterial mycolic acids
Authors: Liu Yi , Kaffah Nadhira , Pandor Sufyan , Sartain Mark J. , Larrouy-Maumus Gerald 
Published: June 2023

AbstractLipids are highly structurally diverse molecules involved in a wide variety of biological processes. The involvement of lipids is even more pronounced in mycobacteria, including the human pathogen Mycobacterium tuberculosis, which produces a highly complex and diverse set of lipids in the cell envelope. These lipids include mycolic acids, which are among the longest fatty acids in nature and can contain up to 90 carbon atoms. Mycolic acids are ubiquitously found in mycobacteria and are alpha branched and beta hydroxylated lipids. Discrete modifications, such as alpha, alpha’, epoxy, methoxy, keto, and carboxy, characterize mycolic acids at the species level. Here, we used high precision ion mobility-mass spectrometry to build a database including 206 mass-resolved collision cross sections (CCSs) of mycolic acids originating from the strict human pathogen M. tuberculosis, the opportunistic strains M. abscessus, M. marinum and M. avium, and the nonpathogenic strain M. smegmatis. Primary differences between the mycolic acid profiles could be observed between mycobacterial species. Acyl tail length and modifications were the primary structural descriptors determining CCS magnitude. As a resource for researchers, this work provides a detailed catalogue of the mass-resolved collision cross sections for mycolic acids along with a workflow to generate and analyse the dataset generated.
Universal and divergent P-stereogenic building with camphor-derived 2,3-diols
Authors: Zhang Yulong , Zhao Peichao , Sun Shengnan , Wu Qian , Shi Enxue , Xiao Junhua 
Published: June 2023

AbstractThe access to P-stereogenic motifs has always been considered a very challenging and high attractive mission in modern organic synthesis. While several chiral auxiliaries employed by the practical Jugé-Stephan-like methodology have been developed, new type of readily accessible bifunctional ligands toward P-stereogenic building still remain much desirable. Herein, we present a powerful chiral template, camphor-derived 2,3-diols named CAMDOL, which were designed and synthesized from the commercially cheap camphorquinone in high yields at 50 grams scale with a column-free purification. Diverse P(III)-chiral compounds and their borane forms including phosphinous acids, phosphinites, and phosphines, as well as the corresponding P(V)-chiral compounds including phosphinates, phosphine oxides, phosphinothioates, phosphine sulfides, and secondary phosphine oxides were afforded in high yields and ee values through the optimal 2,3-diphenyl CAMDOL platform. An unusual C3-OP bond cleavage following the first P-OC2 bond breaking was observed during the ring-opening process when quenching by NH4Cl solution, which generates a unique but valuable camphor-epoxide scaffold as by-product.
Possible episodic infall towards a compact disk in B335
Authors: Bjerkeli Per , Ramsey Jon P. , Harsono Daniel , Plunkett Adele , Li Zhi-Yun , van der Wiel Matthijs H. D. , Calcutt Hannah , Jørgensen Jes K. , Kristensen Lars E. 
Published: June 2023

Context. Previous observations of the isolated Class 0 source B335 have presented evidence of ongoing infall in various molecular lines, such as HCO+, HCN, and CO. There have been no confirmed observations of a rotationally supported disk on scales greater than ~12 au.
Aims. The presence of an outflow in B335 suggests that a disk is also expected to be present or undergoing formation. To constrain the earliest stages of protostellar evolution and disk formation, we aim to map the region where gas falls inwards and observationally constrain its kinematics. Furthermore, we aim to put strong limits on the size and orientation of any disk-like structure in B335.
Methods. We used high angular resolution 13CO data from the Atacama Large Millimeter/submillimeter Array (ALMA) and combined it with shorter-baseline archival data to produce a high-fidelity image of the infall in B335. We also revisited the imaging of high-angular resolution Band 6 continuum data to study the dust distribution in the immediate vicinity of B335.
Results. Continuum emission shows an elliptical structure (10 by 7 au) with a position angle 5 degrees east of north, consistent with the expectation for a forming disk in B335. A map of the infall velocity (as estimated from the 13CO emission), shows evidence of asymmetric infall, predominantly from the north and south. Close to the protostar, infall velocities appear to exceed free-fall velocities. Three-dimensional (3D) radiative transfer models, where the infall velocity is allowed to vary within the infall region, may explain the observed kinematics.
Conclusions. The data suggest that a disk has started to form in B335 and that gas is falling towards that disk. However, kinematically-resolved line data towards the disk itself is needed to confirm the presence of a rotationally supported disk around this young protostar. The high infall velocities we measured are not easily reconcilable with a magnetic braking scenario, suggesting that there is a pressure gradient that allows the infall velocity to vary in the region.