bluuebunny/crossref_metadata_2025_split huggingface dataset:
External validation of the ACC/AHA ASCVD risk score in a Colombian population cohort
Authors: Rodríguez-Ariza Carlos Daniel , Cabrera-Villamizar Alfredo , Rodríguez-Pulido Astrid Lorena , Callegari Santiago , Ossa Rodríguez Natalia Alejandra , Pinilla-Roncancio Mónica , Moreno López Sergio Mauricio , Sánchez-Vallejo Carlos Andrés 
Published: April 2023

AbstractNo cardiovascular risk score has included Latin American patients in its development. The ACC/AHA ASCVD risk score has not been validated in Latin America; consequently, its predictive capacity in the population of the region is unknown. The aim of this study is to evaluate the discrimination capacity and calibration of the ACC/AHA ASCVD score to predict the 10-year risk of a cardiovascular event in a primary prevention cohort followed in a Colombian hospital. A retrospective cohort study was conducted in primary prevention patients belonging to an intermediate/high-risk and low-risk cohort without established atherosclerotic disease. Cardiovascular risk was calculated at inclusion. The calibration was analyzed by comparing observed and expected events in the different risk categories. A discrimination analysis was made using the area under the ROC curve and C statistic. A total of 918 patients were included—202 from the intermediate/high-risk and 716 from the low-risk cohort. The median cardiovascular risk was 3.6% (IQR 1.7–8.5%). At the 10-year follow-up, 40 events (4,4%) occurred. The area under the ROC curve was 0.782 (95% CI 0.71–0.85). The Hosmer–Lemeshow test did not show differences between expected and observed events. The ACC/AHA ASCVD score is calibrated and has good discrimination capacity in predicting 10-year risk of cardiovascular events in a Colombian population.
Pose estimation and motion analysis of ski jumpers based on ECA-HRNet
Authors: Bao Wenxia , Niu Tao , Wang Nian , Yang Xianjun 
Published: April 2023

AbstractSki jumping is a high-speed sport, which makes it difficult to accurately analyze the technical motion in a subjective way. To solve this problem, we propose an image-based pose estimation method for analyzing the motion of ski jumpers. First, an image keypoint dataset of ski jumpers (KDSJ) was constructed. Next, in order to improve the precision of ski jumper pose estimation, an efficient channel attention (ECA) module was embedded in the residual structures of a high-resolution network (HRNet) to fuse more useful feature information. At the training stage, we used a transfer learning method which involved pre-training on the Common Objection in Context (COCO2017) to obtain feature knowledge from the COCO2017 for using in the task of ski jumper pose estimation. Finally, the detected keypoints of the ski jumpers were used to analyze the motion characteristics, using hip and knee angles over time (frames) as an example. Our experimental results showed that the proposed ECA-HRNet achieved the average precision of 73.4% on the COCO2017 test-dev set and the average precision of 86.4% on the KDSJ test set using the ground truth bounding boxes. These research results can provide guidance for auxiliary training and motion evaluation of ski jumpers.
Mechanisms of length-dependent recognition of viral double-stranded RNA by RIG-I
Authors: Im Jung Hyun , Duic Ivana , Yoshimura Shige H. , Onomoto Koji , Yoneyama Mitsutoshi , Kato Hiroki , Fujita Takashi 
Published: April 2023

AbstractRetinoic acid-inducible gene I (RIG-I) is the most front-line cytoplasmic viral RNA sensor and induces antiviral immune responses. RIG-I recognizes short double-stranded (dsRNA) (< 500 bp), but not long dsRNA (> 500 bp) to trigger antiviral signaling. Since RIG-I is capable of binding with dsRNA irrespective of size, length-dependent RIG-I signaling remains elusive. Here, we demonstrated that RIG-I bound to long dsRNA with slow kinetics. Remarkably, RIG-I/short dsRNA complex efficiently dissociated in an ATP hydrolysis-dependent manner, whereas RIG-I/long dsRNA was stable and did not dissociate. Our study suggests that the dissociation of RIG-I from RIG-I/dsRNA complex could be a step for efficient antiviral signaling. Dissociated RIG-I exhibited homo-oligomerization, acquiring ability to physically associate with MAVS, and biological activity upon introduction into living cells. We herein discuss common and unique mechanisms of viral dsRNA recognition by RIG-I and MDA5.
Serial evaluation of the serum lactate level with the SOFA score to predict mortality in patients with sepsis
Authors: Park Heemoon , Lee Jinwoo , Oh Dong Kyu , Park Mi Hyeon , Lim Chae-Man , Lee Sang-Min , Lee Hong Yeul ,   the Korean Sepsis Alliance (KSA) Investigators
Published: April 2023

AbstractIn patients with sepsis, outcome prediction plays an important role in influencing therapeutic decision making. In this nationwide, prospective, observational cohort study of sepsis patients conducted between September 2019 and December 2020, we evaluated a novel scoring system using serial Sequential Organ Failure Assessment (SOFA) scores and serum lactate to accurately predict mortality in sepsis. Based on the serum lactate score (Lac-score), patients were assigned to 5 categories: lactate < 2, ≥ 2 to < 4, ≥ 4 to < 8, ≥ 8 to < 12, and ≥ 12 mmol/L. Lac-SOFA score was defined as the sum of Lac-score and SOFA score. After screening 7113 patients, 379 were excluded and 6734 were included in analysis. In-hospital mortality AUROC for serial Lac-SOFA score from initial to ICU day 3 was significantly higher than that for serial SOFA score (initial, 0.679 vs. 0.656, day 1, 0.723 vs. 0.709, day 2, 0.760 vs. 0.747, and day 3, 0.797 vs. 0.781; DeLong's test, p < 0.001). The initial Lac-SOFA score significantly correlated with in-hospital mortality when the patients were divided into five classes based on 5-point intervals (p < 0.05). Serial evaluation of lactate levels with the SOFA score may improve the predictive accuracy of the SOFA score for determining mortality risk in sepsis patients.
Adherence to oxidative balance scores and lower odds of non-alcoholic fatty liver disease: a case–control study
Authors: Sohouli Mohammad Hassan , Rohani Pejman , Hosseinzadeh Mahdieh , Hekmatdoost Azita 
Published: April 2023

AbstractEvidence has also shown that oxidative stress and systemic inflammation, or in other words, disruption of the oxidant and antioxidant balance, can play an important role in the initiation or progression of NAFLD. The purpose of this study was to investigate the associations between the oxidative balance scores (OBS) and the risk of NAFLD. 552 healthy and 340 patients adult over the age of 18 with NAFLD participated in this case–control research. A validated 168-item quantitative food frequency questionnaire (FFQ) and indicators of physical activity, obesity, and smoking status were used to assess OBS score. The connection between OBS and NAFLD was discovered using binary logistic regression. The mean (± SD) age and (body mass index) BMI of the study population was 40.22 ± 9.79 years and 29.06 ± 3.92 kg/m2, respectively. The mean ± SD of OBS was 41.48 ± 5.23. After adjustment for potential confounders, higher scores of adherence to the OBS conferred a protection for the presence of NAFLD (odds ratio [OR]: 0.29; 95% confidence interval [CI]: 0.15–0.49; P for trend < 0.001). The findings of the present study indicate an approximately 80% reduction in the odds of developing NAFLD with higher OBS adherence in the overall population. However, prospective studies are needed to further investigate this association.
The response to stressors in adulthood depends on the interaction between prenatal exposure to glucocorticoids and environmental context
Authors: Majer Ariana D. , Paitz Ryan T. , Tricola Gianna M. , Geduldig Jack E. , Litwa Hannah P. , Farmer Jenna L. , Prevelige Brenna R. , McMahon Elyse K. , McNeely Taylor , Sisson Zach R. , Frenz Brian J. , Ziur Alexis D. , Clay Emily J. , Eames Brad D. , McCollum Shannon E. , Haussmann Mark F. 
Published: April 2023

AbstractMaternal stress during reproduction can influence how offspring respond to stress later in life. Greater lifetime exposure to glucocorticoid hormones released during stress is linked to greater risks of behavioral disorders, disease susceptibility, and mortality. The immense variation in individual’s stress responses is explained, in part, by prenatal glucocorticoid exposure. To explore the long-term effects of embryonic glucocorticoid exposure, we injected Japanese quail (Coturnix japonica) eggs with corticosterone. We characterized the endocrine stress response in offspring and measured experienced aggression at three different ages. We found that prenatal glucocorticoid exposure affected (1) the speed at which the stress response was terminated suggesting dysregulated negative feedback, (2) baseline corticosterone levels in a manner dependent on current environmental conditions with higher levels of experienced aggression associated with higher levels of baseline corticosterone, (3) the magnitude of an acute stress response based on baseline concentrations. We finish by proposing a framework that can be used to test these findings in future work. Overall, our findings suggest that the potential adaptive nature of prenatal glucocorticoid exposure is likely dependent on environmental context and may also be tempered by the negative effects of longer exposure to glucocorticoids each time an animal faces a stressor.
Novel superpixel method to visualize fundus blood flow resistivity in healthy adults
Authors: Okamoto Kenji , Takahashi Noriyoshi , Kobayashi Tatsuhiko , Shiba Tomoaki , Hori Yuichi , Fujii Hitoshi 
Published: April 2023

AbstractWe aimed to perform superpixel segmentation of ocular blood flow maps obtained using laser speckle flowgraphy (LSFG) and investigate the effects of systemic parameters such as body weight, height, and sex on ocular blood flow resistivity. We studied 757 healthy participants (583 men, 174 women). We calculated the average beat strength over mean blur rate (BOM) as a LSFG resistivity index, as a function of age and sex using ordinary regions of interest (ROI) centered on the optic nerve head (ONH), the retinal vessels region and tissue around the ONH, and the choroid (CHD). We compared the ROI and superpixel-based methods, which are segmented based on image processing, for calculating the BOM. The sex differences in the BOM for the ONH, retinal-vessels region and tissue region of the ONH and CHD were significant for individuals aged ≤ 50 years (P < 0.01) but not those > 50 years old (P > 0.05). The average BOMs calculated using the ROI and superpixel methods were strongly correlated in the ONH (coefficient = 0.87,R2 = 0.8,P < 0.0001,n = 5465). In summary, a superpixel-segmented BOM map is suitable for two-dimensional visualization of ocular blood flow resistivity.
Perceiving the representative surface color of real-world materials
Authors: Zhang Yan , Motoyoshi Isamu 
Published: April 2023

AbstractNatural surfaces such as soil, grass, and skin usually involve far more complex and heterogenous structures than the perfectly uniform surfaces assumed in studies on color and material perception. Despite this, we can easily perceive the representative color of these surfaces. Here, we investigated the visual mechanisms underlying the perception of representative surface color using 120 natural images of diverse materials and their statistically synthesized images. Our matching experiments indicated that the perceived representative color revealed was not significantly different from the Portilla–Simoncelli-synthesized images or phase-randomized images except for one sample, even though the perceived shape and material properties were greatly impaired in the synthetic stimuli. The results also showed that the matched representative colors were predictable from the saturation-enhanced color of the brightest point in the image, excluding the high-intensity outliers. The results support the notion that humans judge the representative color and lightness of real-world surfaces depending on simple image measurements.
Method and application of information sharing throughout the emergency rescue process based on 5G and AR wearable devices
Authors: Wang Mengying , Ji Hong , Jia Mo , Sun Zhen , Gu Jinyi , Ren Haiying 
Published: April 2023

AbstractThe 2022 Winter Olympics were held in the three competition zones of Beijing, Yanqing and Zhangjiakou, China. The venues of this Winter Olympics were scattered and the terrain was complex. Moreover, the medical resources of Hebei and Beijing were relatively unbalanced. In the medical security of major events, the connection between first aid and in-hospital processes is of the utmost importance to rescue quality. 5th generation mobile network (5G) applications in medical scenarios are on the rise. It would be of great relevance to fully use 5G’s low-latency and high-speed features to share the process information of patients, ambulance personnel, and the destination hospital’s rescue team at emergency scenes and in transportation, improving rescue efficiency. This paper proposes a system scheme of cross-institutional emergency health information sharing based on 5G and augmented reality wearable devices. It also integrates the construction method of monitoring and other sign data sharing, in addition to testing the proposed scheme’s service quality in 5G environments. In the deployment area of the 5G emergency medical rescue information sharing scheme for the Beijing Winter Olympic Games, we selected two designated medical support institutions for testing. The test adopted a combination of fixed-point and driving tests to experiment on the service data, voice service, and streaming media indicators. The 5G signal's coverage rate was close to 100%, the standalone connection's success rate was 100%, and the drop rate was 0. The average downlink rate of multiple scenarios was 620mbps, and the average uplink rate of 5G was over 71.8mbps, which is higher than the average 5G level in China. The downlink rate was more than 20 times larger than the 4th generation mobile network (4G) rate. This study’s proposed scheme demonstrates the importance of 5G applications in emergency response and support, in addition to providing a suitable scheme for the integration of 5G networks in the medical scene.
A p-orbital honeycomb-Kagome lattice realized in a two-dimensional metal-organic framework
Authors: Wang Xiao-Bo , Xia Bowen , Lyu Cheng-Kun , Kim Dongwook , Li En , Fu Shu-Qing , Chen Jia-Yan , Liu Pei-Nian , Liu Feng , Lin Nian 
Published: April 2023

AbstractThe experimental realization ofp-orbital systems is desirable becausep-orbital lattices have been proposed theoretically to host strongly correlated electrons that exhibit exotic quantum phases. Here, we synthesize a two-dimensional Fe-coordinated bimolecular metal-organic framework which constitutes a honeycomb lattice of 1,4,5,8,9,12-hexaazatriphenylene molecules and a Kagome lattice of 5,15-di(4-pyridyl)-10,20-diphenylporphyrin molecules on a Au(111) substrate. Density-functional theory calculations show that the framework features multiple well-separated spin-polarized Kagome bands, namely Dirac cone bands and Chern flat bands, near the Fermi level. Using tight-binding modelling, we reveal that these bands are originated from two effects: the low-lying molecular orbitals that exhibitp-orbital characteristics and the honeycomb-Kagome lattice. This study demonstrates thatp-orbital Kagome bands can be realized in metal-organic frameworks by using molecules with molecular orbitals ofp-orbital like symmetry.
Identifying where nature-based solutions can offer win-wins for carbon mitigation and biodiversity across knowledge systems
Authors: Raymond Christopher M. , Lechner Alex M. , Havu Minttu , Jalkanen Joel , Lampinen Jussi , Antúnez Oriol García , Olafsson Anton Stahl , Gulsrud Natalie , Kinnunen Antti , Backman Leif , Kulmala Liisa , Järvi Leena 
Published: April 2023

AbstractManaging nature-based solutions (NBS) in urban areas for carbon mitigation and biodiversity outcomes is a global policy challenge, yet little is known about how to both assess and weave diverse knowledge systems and values into carbon-biodiversity trade-off assessments. This paper examines the spatial relationships between biophysical and social values for carbon sequestration potential (measured as carbon dioxide, CO2, flux) and biodiversity in Helsinki, Finland, using integrated valuation. The approach combines methods from carbon sequestration modelling, expert scoring approaches to biodiversity assessment and public participation geographic information systems (PPGIS). Results indicate strong spatial associations between biophysical assessment of CO2flux and biodiversity priorities, and weaker associations between biophysical and social values. Integration of social and biophysical values leads to multiple pathways for protection of NBS to achieve carbon mitigation and biodiversity outcomes, as well as options for the spatial targeting of education and capacity building programs to areas of local concern.
Intensified livestock farming increases antibiotic resistance genotypes and phenotypes in animal feces
Authors: Wang Hang , Qi Jin-Feng , Qin Rong , Ding Kai , Graham David W. , Zhu Yong-Guan 
Published: April 2023

AbstractAnimal feces from livestock farming can be a major source of antibiotic resistance to the environment, but a clear gap exists on how the resistance reservoir in feces alters as farming activities intensify. Here, we sampled feces from eight Chinese farms, where yak, sheep, pig, and horse were reared under free-range to intensive conditions, and determined fecal resistance using both genotype and phenotype approaches. Animals reared intensively exhibited increased diversity of antibiotic resistance genes and greater resistance phenotypes in feces, which were cross-correlated. Furthermore, at the metagenome contig level, antibiotic resistance genes were co-located with mobile genetic elements at a higher frequency (27.38%) as farming intensified, with associated resistance phenotypes being less coupled with bacterial phylogeny. Intensified farming also expanded the multidrug resistance preferentially carried on pathogens in fecal microbiomes. Overall, farming intensification can increase antibiotic resistance genotypes and phenotypes in domestic animal feces, with implications for environmental health.
Lytic polysaccharide monooxygenases: enzymes for controlled and site-specific Fenton-like chemistry
Authors: Bissaro Bastien , Eijsink Vincent G.H. 
Published: April 2023

Abstract
The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has profoundly changed our current understanding of enzymatic processes underlying the conversion of polysaccharides in the biosphere. LPMOs are truly unique enzymes, harboring a single copper atom in a solvent-exposed active site, allowing them to oxidize C-H bonds at the C1 and/or C4 carbon of glycosidic linkages found in recalcitrant, often crystalline polysaccharides such as cellulose and chitin. To catalyze this challenging reaction, LPMOs harness and control a powerful oxidative reaction that involves Fenton-like chemistry. In this essay, we first draw a brief portrait of the LPMO field, notably explaining the shift from the monooxygenase paradigm (i.e., using O2 as cosubstrate) to that of a peroxygenase (i.e., using H2O2). Then, we briefly review current understanding of how LPMOs generate and control a hydroxyl radical (HO•) generated through Cu(I)-catalyzed H2O2 homolysis, and how this radical is used to create the proposed Cu(II)-oxyl species, abstracting hydrogen atom of the C-H bond. We also point at the complexity of analyzing redox reactions involving reactive oxygen species and address potential deficiencies in the interpretation of existing LPMO data. Being the first copper enzymes shown to enable site-specific Fenton-like chemistry, and maybe not the only ones, LPMOs may serve as a blueprint for future research on monocopper peroxygenases.
A special issue of Essays in Biochemistry on current advances about CAZymes and their impact and key role in human health and environment
Authors: Czjzek Mirjam , Ficko-Blean Elizabeth , Berrin Jean-Guy 
Published: April 2023

Abstract
Carbohydrate active enzymes (CAZymes) and their biochemical characterization have been the subject of extensive research over the past ten years due to their importance to carbohydrate metabolism in different biological contexts. For instance, the understanding that ‘polysaccharide utilizing loci’ (PUL) systems hosted by specific ‘carbohydrate degraders’ in the intestinal microbiota play key roles in health and disease, such as Crohn’s disease, ulcerative colitis or colorectal cancer to name the most well-characterized, has led to an outstanding effort in trying to decipher the molecular mechanisms by which these processes are organized and regulated.
The past 10 years has also seen the expansion of CAZymes with auxiliary activities, such as lytic polysaccharide monooxygenases (LPMOs) or even sulfatases, and interest has grown in general about the enzymes needed to remove the numerous decorations and modifications of complex biomass, such as carbohydrate esterases (CE). Today, the characterization of these ‘modifying’ enzymes allows us to tackle a much more complex biomass, which presents sulfations, methylations, acetylations or interconnections with lignin.
This special issue about CAZyme biochemistry covers all these aspects, ranging from implications in disease to environmental and biotechnological impact, with a varied collection of twenty-four review articles providing current biochemical, structural and mechanistic insights into their respective topics.
Modeling Responses to Auditory Feedback Perturbations in Adults, Children, and Children With Complex Speech Sound Disorders: Evidence for Impaired Auditory Self-Monitoring?
Authors: Terband Hayo , van Brenk Frits 
Published: April 2023

Purpose:
Previous studies have found that typically developing (TD) children were able to compensate and adapt to auditory feedback perturbations to a similar or larger degree compared to young adults, while children with speech sound disorder (SSD) were found to produce predominantly following responses. However, large individual differences lie underneath the group-level results. This study investigates possible mechanisms in responses to formant shifts by modeling parameters of feedback and feedforward control of speech production based on behavioral data.

Method:
SimpleDIVA was used to model an existing dataset of compensation/adaptation behavior to auditory feedback perturbations collected from three groups of Dutch speakers: 50 young adults, twenty-three 4- to 8-year-old children with TD speech, and seven 4- to 8-year-old children with SSD. Between-groups and individual within-group differences in model outcome measures representing auditory and somatosensory feedback control gain and feedforward learning rate were assessed.

Results:
Notable between-groups and within-group variation was found for all outcome measures. Data modeled for individual speakers yielded model fits with varying reliability. Auditory feedback control gain was negative in children with SSD and positive in both other groups. Somatosensory feedback control gain was negative for both groups of children and marginally negative for adults. Feedforward learning rate measures were highest in the children with TD speech followed by children with SSD, compared to adults.

Conclusions:
The SimpleDIVA model was able to account for responses to the perturbation of auditory feedback other than corrective, as negative auditory feedback control gains were associated with following responses to vowel shifts. These preliminary findings are suggestive of impaired auditory self-monitoring in children with complex SSD. Possible mechanisms underlying the nature of following responses are discussed.