bluuebunny/crossref_metadata_2025_split huggingface dataset:
The true natural cycle frozen embryo transfer - impact of patient and follicular phase characteristics on serum progesterone levels one day prior to warmed blastocyst transfer
Authors: Mumusoglu Sezcan , Erden Murat , Ozbek Irem Yarali , Ince Onur , Esteves Sandro C. , Humaidan Peter , Yarali Hakan 
Published: September 2023

Abstract
Background
In a true-natural cycle (t-NC), optimal progesterone (P4) output from the corpus luteum is crucial for establishing and maintaining an intrauterine pregnancy. In a previous retrospective study, low P4 levels (< 10 ng/mL) measured one day before warmed blastocyst transfer in t-NC were associated with significantly lower live-birth rates. In the current study, we aim to examine the relationship between patient, follicular-phase endocrine and ultrasonographic characteristics, and serum P4 levels one day prior to warmed blastocyst transfer in t-NC.

Method
178 consecutive women undergoing their first t-NC frozen embryo transfer (FET) between July 2017-August 2022 were included. Following serial ultrasonographic and endocrine monitoring, ovulation was documented by follicular collapse. Luteinized unruptured follicle (LUF) was diagnosed when there was no follicular collapse despite luteinizing-hormone surge (> 17 IU/L) and increased serum P4 (> 1.5 ng/mL). FET was scheduled on follicular collapse + 5 or LH surge + 6 in LUF cycles. Primary outcome was serum P4 on FET − 1.

Results
Among the 178 patients, 86% (n = 153) experienced follicular collapse, while 14% (n = 25) had LUF. On FET-1, the median serum luteal P4 level was 12.9 ng/mL (IQR: 9.3–17.2), ranging from 1.8 to 34.4 ng/mL. Linear stepwise regression revealed a negative correlation between body mass index (BMI) and LUF, and a positive correlation between follicular phase peak-E2 and peak-P4 levels with P4 levels on FET-1. The ROC curve analyses to predict < 9.3 ng/mL (< 25th percentile) P4 levels on FET-1 day showed AUC of 0.70 (95%CI 0.61–0.79) for BMI (cut-off: 23.85 kg/m2), 0.71 (95%CI 0.61–0.80) for follicular phase peak-P4 levels (cut-off: 0.87 ng/mL), and 0.68 (95%CI 0.59–0.77) for follicular phase peak-E2 levels (cut-off: 290.5 pg/mL). Combining all four independent parameters yielded an AUC of 0.80 (95%CI 0.72–0.88). The adjusted-odds ratio for having < 9.3 ng/mL P4 levels on FET-1 day for patients with LUF compared to those with follicle collapse was 4.97 (95%CI 1.66–14.94).

Conclusion
The BMI, LUF, peak-E2, and peak-P4 levels are independent predictors of low serum P4 levels on FET-1 (< 25th percentile; <9.3 ng/ml) in t-NC FET cycles. Recognition of risk factors for low serum P4 on FET-1 may permit a personalized approach for LPS in t-NC FET to maximize reproductive outcomes.
Fibroblast diversity and plasticity in the tumor microenvironment: roles in immunity and relevant therapies
Authors: Xu Yashi , Li Wei , Lin Shitong , Liu Binghan , Wu Peng , Li Li 
Published: September 2023

AbstractCancer-associated fibroblasts (CAFs), enriched in the tumor stroma, have received increasing attention because of their multifaceted effects on tumorigenesis, development, metastasis, and treatment resistance in malignancies. CAFs contributed to suppressive microenvironment via different mechanisms, while CAFs also exerted some antitumor effects. Therefore, CAFs have been considered promising therapeutic targets for their remarkable roles in malignant tumors. However, patients with malignancies failed to benefit from current CAFs-targeted drugs in many clinical trials, which suggests that further in-depth investigation into CAFs is necessary. Here, we summarize and outline the heterogeneity and plasticity of CAFs mainly by exploring their origin and activation, highlighting the regulation of CAFs in the tumor microenvironment during tumor evolution, as well as the critical roles performed by CAFs in tumor immunity. In addition, we summarize the current immunotherapies targeting CAFs, and conclude with a brief overview of some prospects for the future of CAFs research in the end.
Dysregulation of inflammasome activation in glioma
Authors: Sim JeongMin , Park JeongMan , Moon Jong-Seok , Lim Jaejoon 
Published: September 2023

AbstractGliomas are the most common brain tumors characterized by complicated heterogeneity. The genetic, molecular, and histological pathology of gliomas is characterized by high neuro-inflammation. The inflammatory microenvironment in the central nervous system (CNS) has been closely linked with inflammasomes that control the inflammatory response and coordinate innate host defenses. Dysregulation of the inflammasome causes an abnormal inflammatory response, leading to carcinogenesis in glioma. Because of the clinical importance of the various physiological properties of the inflammasome in glioma, the inflammasome has been suggested as a promising treatment target for glioma management. Here, we summarize the current knowledge on the contribution of the inflammasomes in glioma and therapeutic insights.
Perioperative safety and efficacy of robot-assisted total hip arthroplasty in ERAS-managed patients: a pilot study
Authors: Lu Hanpeng , Sun Haocheng , Xiao Qiang , Xu Hong , Zhou Qi , Li Linyuan , Yan Tingfang , Wang Duan , Zhou Zongke 
Published: September 2023

Abstract
Aims
Robot-assisted total hip arthroplasty (rTHA) boasts superior accuracy in implant placement, but there is a lack of effective assessment in perioperative management in the context of enhanced recovery after surgery (ERAS). This study aimed to compare the effectiveness and safety of rTHA versus conventional total hip arthroplasty (cTHA) in ERAS-managed patients.

Methods
In this prospective trial, a total of 60 eligible patients aged between 18 and 80 years were randomly divided into two groups to undergo either rTHA or cTHA. The primary outcomes included blood loss parameters. Secondary outcomes were the duration of the operation, surgical time, WOMAC pain score, WOMAC stiffness score, WOMAC physical function score, Harris score, and postoperative complications.

Results
The study cohort analyzed 59 eligible participants, 30 of whom underwent rTHA and 29 of whom underwent cTHA. Analysis could not be conducted for one patient due to severe anemia. Notably, the cTHA group had a significantly shorter surgical time than the rTHA group (69.49 ± 18.97 vs. 104.20 ± 19.63 min, P < 0.001). No significant differences were observed between the rTHA and cTHA groups for blood loss parameters, including total blood loss (1280.30 ± 404.01 vs. 1094.86 ± 494.39 ml, P = 0.137) and drainage volume (154.35 ± 121.50 vs. 159.13 ± 135.04 ml, P = 0.900), as well as intraoperative blood loss (126.67 ± 38.80 vs. 118.52 ± 60.68 ml, P = 0.544) and hidden blood loss (982.43 ± 438.83 vs. 784.00 ± 580.96 ml, P = 0.206). Only one patient in the cTHA group required allogeneic blood transfusion. At 3 months postoperatively, both groups showed improvements in WOMAC pain score, WOMAC stiffness score, WOMAC physical function score, and Harris score, with no significant differences found between the two groups. Few complications were reported in both groups without significant differences.

Conclusions
Despite the longer surgical time, rTHA did not negatively affect blood loss, pain, or functional recovery or lead to an increased risk of complications in ERAS-managed patients, suggesting that rTHA can be safely and effectively incorporated into an ERAS program for primary THA.
Risk stratification tools for patients with syncope in emergency medical services and emergency departments: a scoping review
Authors: uit het Broek Lucia G. , Ort B. Bastiaan A. , Vermeulen Hester , Pelgrim Thomas , Vloet Lilian C.M. , Berben Sivera A.A. 
Published: September 2023

Abstract
Background
Patients with a syncope constitute a challenge for risk stratification in (prehospital) emergency care. Professionals in EMS and ED need to differentiate the high-risk from the low-risk syncope patient, with limited time and resources. Clinical decision rules (CDRs) are designed to support professionals in risk stratification and clinical decision-making. Current CDRs seem unable to meet the standards to be used in the chain of emergency care. However, the need for a structured approach for syncope patients remains. We aimed to generate a broad overview of the available risk stratification tools and identify key elements, scoring systems and measurement properties of these tools.

Methods
We performed a scoping review with a literature search in MEDLINE, CINAHL, Pubmed, Embase, Cochrane and Web of Science from January 2010 to May 2022. Study selection was done by two researchers independently and was supervised by a third researcher. Data extraction was performed through a data extraction form, and data were summarised through descriptive synthesis. A quality assessment of included studies was performed using a generic quality assessment tool for quantitative research and the AMSTAR-2 for systematic reviews.

Results
The literature search identified 5385 unique studies; 38 were included in the review. We discovered 19 risk stratification tools, one of which was established in EMS patient care. One-third of risk stratification tools have been validated. Two main approaches for the application of the tools were identified. Elements of the tools were categorised in history taking, physical examination, electrocardiogram, additional examinations and other variables. Evaluation of measurement properties showed that negative and positive predictive value was used in half of the studies to assess the accuracy of tools.

Conclusion
A total of 19 risk stratification tools for syncope patients were identified. They were primarily established in ED patient care; most are not validated properly. Key elements in the risk stratification related to a potential cardiac problem as cause for the syncope. These insights provide directions for the key elements of a risk stratification tool and for a more advanced process to validate risk stratification tools.
A fluorescence-based assay for Trichomonas vaginalis drug screening
Authors: Chen Qianqian , Li Jingzhong , Wang Zhensheng , Meng Wei , Wang Heng , Wang Zenglei 
Published: September 2023

Abstract
Background
The emergence and spread of drug resistance in Trichomonas vaginalis parasites has become an important concern in trichomoniasis treatment. Fast and reliable growth assessment is critical for validating in vitro drug susceptibility and high-throughput screening of newly developed drugs.

Methods
Modified media without yeast extract were evaluated for their ability to support the growth of T. vaginalis parasites. The potential of the nucleic acid-binding dye SYBR Green I for detecting T. vaginalis drug resistance was characterized, and seeding parasite concentration and incubation time were optimized. The fluorescence assay based on SYBR Green I was further validated in four T. vaginalis isolates with different susceptibilities to the antibiotics metronidazole, tinidazole, ornidazole and secnidazole, and compared with the traditional method that detects minimum lethal concentrations (MLCs).

Results
A modified medium consisting of RPMI 1640 and Tryptone Plus as replacements for yeast extract and tryptone, respectively, in traditional trypticase-yeast extract-maltose (TYM) medium exhibited similar performance as TYM medium in maintaining T. vaginalis growth, while it showed much lower background fluorescent signals. The T. vaginalis SYBR Green I-based fluorescence (TSF) drug assay was found to have to satisfy one of two conditions to demonstrate the 50% inhibitory concentration of metronidazole for the sensitive isolate TV-334: (i) a seeding density of 3 × 104 parasites/ml and an incubation time of 48 h; or (ii) a seeding density of 1 × 104 parasites/ml and an incubation time of 72 h. Subsequent validation experiments revealed that the 48-h incubation/3 × 104 parasites/ml seeding density condition had a greater sensitivity to detect drug resistance than the 72-h condition. The TSF assay also exhibited high efficiency in identifying parasite drug resistance, as evidenced by its strong correlation with the standard MLC assay results (P = 0.003).

Conclusions
This study presents a robust TSF assay that has the potential to facilitate high-throughput, automated in vitro anti-trichomoniasis susceptibility testing for drug resistance monitoring and drug development. In comparison to the standard MLC method, this assay offers the advantages of reduced labor and elimination of subjective examination.

Graphical Abstract
On generalization of Levinson’s inequality involving averages of 3-convex functions
Authors: Aras-Gazić G. , Pečarić J. , Vukelić A. 
Published: September 2023

AbstractBy using an integral arithmetic mean, a generalization of Levinson’s inequality given in (Pečarić et al. in Convex Functions, Partial Orderings, and Statistical Applications. Mathematics in Science and Engineering, vol. 187, 1992) and results from (Vukelić in Appl. Anal. Discrete Math. 14:670–684, 2020), we give extension of Wulbert’s result from (Wulbert in Math. Comput. Model. 37:1383–1391, 2003). Also, we obtain inequalities with divided differences for the functions of higher order.
Soil fauna accelerated litter C and N release by improving litter quality across an elevational gradient
Authors: Zhang Li , Liu Jingru , Yin Rui , Xu Zhenfeng , You Chengming , Li Han , Wang Lixia , Liu Sining , Xu Hongwei , Xu Lin , Liu Yang , Wang Yong , Tan Bo 
Published: September 2023

Abstract
Background
Soil fauna is an important driver of carbon (C) and nitrogen (N) release from decomposing litter in forest ecosystems. However, its role in C and N cycling concerning climate and litter traits remains less known. In a 4-year field experiment, we evaluated the effects of soil fauna on litter C and N release across an elevation gradient (453, 945, 3023, and 3582 m) and litter traits (coniferous vs. broadleaf) in southwestern China.

Results
Our results showed that N was retained by –0.4% to 31.5%, but C was immediately released during the early stage (156–516 days) of decomposition for most litter species. Soil fauna significantly increased the peak N content and N retention across litter species, but reduced the C/N ratio for certain species (i.e., Juniperus saltuaria, Betula albosinensis, Quercus acutissima, and Pinus massoniana litter), leading to more C and N being released from decomposing litter across the elevation gradient. Contributions of soil fauna to C and N release were 3.87–9.90% and 1.10–8.71%, respectively, across litter species after 4 years of decomposition. Soil environment and initial litter quality factors caused by elevation directly affected litter C and N release. Changes in soil fauna resulting from elevation and fauna exclusion factors had a direct or indirect impact on C and N release during litter decomposition.

Conclusions
Our findings suggest that soil fauna promote C and N release from decomposing litter in different magnitudes, mainly controlled by environmental conditions (i.e., temperature and moisture), litter quality (i.e., lignin and cellulose content, and lignin/cellulose), and its diversity across the elevation gradient.
National prevalence and risk factors for tungiasis in Kenya
Authors: Elson Lynne , Kamau Christopher , Koech Sammy , Muthama Christopher , Gachomba George , Sinoti Erastus , Chondo Elwyn , Mburu Eliud , Wakio Miriam , Lore Jimmy , Maia Marta , Adetifa Ifedayo , Orindi Benedict , Bejon Phillip , Fillinger Ulrike 
Published: September 2023

Abstract
Background
Tungiasis is a highly neglected tropical skin disease caused by the sand flea, Tunga penetrans, the female of which burrows into the skin, causing pain and itching. The disease occurs throughout South America and sub-Saharan Africa but there are few systematic data on national disease burdens. The tungiasis research community is keen to develop survey methods to fill this gap. Here we used a school-based, thorough examination method to determine the prevalence and risk factors for tungiasis in Kenya.

Methods
We conducted the first nationally representative survey of tungiasis, including nine counties covering the major ecological zones of Kenya. A stratified multistage random sampling was used to select 22 primary schools from each of the nine counties and to select up to 114 pupils aged 8 to 14 years in each school. Pupils were examined thoroughly for tungiasis. Two surveys were conducted, the first between May and July 2021 and the second between October 2021 and April 2023 when pupils were also interviewed for risk factors. Mixed effect logistic regression models were used to test associations of independent variables with tungiasis using the school as a random effect.

Results
The overall prevalence of tungiasis in the first survey was 1.35% [95% confidence interval (CI): 1.15–1.59%], and 0.89% in the second survey. The prevalence ranged from 0.08% (95% CI: 0.01–0.59%) in Taita Taveta county to 3.24% (95% CI: 2.35–4.44%) in Kajiado county. Tungiasis infection was associated with county of residence, male sex [adjusted odds ratio (aOR) = 2.01, 95% CI: 1.52–2.67], and lower age (aOR = 0.81, 95% CI: 0.75–0.88). For the first time we demonstrate an association with attending public schools rather than private schools (aOR = 5.62, 95% CI: 1.20–26.22) and lower socioeconomic status (aOR = 0.10, 95% CI: 0.03–0.33). Using a rapid screening method of the top of feet only, would have missed 62.9% of all cases, 78.9% of mild cases and 20.0% of severe cases.

Conclusions
Tungiasis is widely but heterogeneously distributed across Kenya. School-based surveys offer an efficient strategy for mapping tungiasis distribution.

Graphical Abstract
Psychometric properties of an Arabic translation of the body appreciation scale (BAS-2) and its short forms (BAS-2SF) in a community sample of Lebanese adults
Authors: Fekih-Romdhane Feten , Azzi Vanessa , Malaeb Diana , Sarray El Dine Abir , Obeid Sahar , Hallit Souheil 
Published: September 2023

AbstractObjectivesThe present study sought to examine the factor structure, reliability, validity and gender invariance of the Arabic Body Appreciation Scale (BAS-2), and its two short forms (the 3-item and 2-item BAS-2SF) among community Lebanese Arabic-speaking adults.MethodsWe carried-out an online cross-sectional survey. The 10-item BAS-2, the functionality appreciation scale and the Eating Attitudes Test-26 were completed by 826 Lebanese community adults (57.9% females, aged 25.42 ± 8.44 years).ResultsThe Arabic 10-item, 3-item and 2-item BAS-2 converged on a one-factor solution, and demonstrated high internal consistency (McDonald’s ω value of .92, .88, and .86 respectively). All indices suggested that configural, metric, and scalar invariance was supported across gender. The 3-item and 2-item BAS-2SF were highly correlated with the original BAS-2 (r > 0.9). Higher 10-item, 3-item and 2-item BAS-2 scores correlated significantly and strongly with more positive eating attitudes and greater functionality appreciation, and higher 10-item BAS-2 scores correlated positively and weakly with BMI, supporting convergent validity.ConclusionThe present work contributes to the literature by providing a psychometrically sound Arabic-translation of the BAS-2 and short-forms, benefitting both researchers and clinicians.
A toxic gain-of-function mechanism in C9orf72 ALS impairs the autophagy-lysosome pathway in neurons
Authors: Beckers Jimmy , Tharkeshwar Arun Kumar , Fumagalli Laura , Contardo Matilde , Van Schoor Evelien , Fazal Raheem , Thal Dietmar Rudolf , Chandran Siddharthan , Mancuso Renzo , Van Den Bosch Ludo , Van Damme Philip 
Published: September 2023

Abstract
Background
Motor neurons (MNs), which are primarily affected in amyotrophic lateral sclerosis (ALS), are a specialized type of neurons that are long and non-dividing. Given their unique structure, these cells heavily rely on transport of organelles along their axons and the process of autophagy to maintain their cellular homeostasis. It has been shown that disruption of the autophagy pathway is sufficient to cause progressive neurodegeneration and defects in autophagy have been associated with various subtypes of ALS, including those caused by hexanucleotide repeat expansions in the C9orf72 gene. A more comprehensive understanding of the dysfunctional cellular mechanisms will help rationalize the design of potent and selective therapies for C9orf72-ALS.

Methods
In this study, we used induced pluripotent stem cell (iPSC)-derived MNs from C9orf72-ALS patients and isogenic control lines to identify the underlying mechanisms causing dysregulations of the autophagy-lysosome pathway. Additionally, to ascertain the potential impact of C9orf72 loss-of-function on autophagic defects, we characterized the observed phenotypes in a C9orf72 knockout iPSC line (C9-KO).

Results
Despite the evident presence of dysfunctions in several aspects of the autophagy-lysosome pathway, such as disrupted lysosomal homeostasis, abnormal lysosome morphology, inhibition of autophagic flux, and accumulation of p62 in C9orf72-ALS MNs, we were surprised to find that C9orf72 loss-of-function had minimal influence on these phenotypes. Instead, we primarily observed impairment in endosome maturation as a result of C9orf72 loss-of-function. Additionally, our study shed light on the pathological mechanisms underlying C9orf72-ALS, as we detected an increased TBK1 phosphorylation at S172 in MNs derived from C9orf72 ALS patients.

Conclusions
Our data provides further insight into the involvement of defects in the autophagy-lysosome pathway in C9orf72-ALS and strongly indicate that those defects are mainly due to the toxic gain-of-function mechanisms underlying C9orf72-ALS.
Cycling knee brace design analysis using 3D virtual clothing program to assess clothing pressure distribution and variance
Authors: Kim Hee Young , Oh Kyung Wha 
Published: September 2023

AbstractThis study analyzed the functional design by investigating the distribution of clothing pressure of cycling knee brace using a three-dimensional virtual clothing program. Based on the average body size of Korean men in their 40 s, clothing pressure in wearing and cycling condition were collected on five knee brace products. According to the results, bonding fabric products had a high possibility of increasing inconvenience, as they had a higher clothing pressure at all measurement points and soared in motion application. It could be adjusted differently depending on the location by mixing materials or using details, so the design with thick pile fabric or velcro strap fastening added more pressure to the lower part of the knee. The length and circumference size also affected the clothing pressure. The design with tight upper and lower circumference pressured excessively the legs and rolled up and clumped together, while the one with loose lower circumference lacked the sufficient strength to support the knees. Also, the design with a curved outline due to the difference in the length of the front and back, increased the clothing pressure on the lower thigh or the upper calf. Therefore, the functional design of cycling knee braces requires comprehensive consideration of material thickness and structure, detailed size of length and circumference, fastening details, to reduce the pressure at the center and top of the knee and to support the lower part with proper pressure, as reflecting the pressure on the body location that come into contact during the motion.