bluuebunny/crossref_metadata_2025_split huggingface dataset:
Splicing factor TRA2A contributes to esophageal cancer progression via a noncanonical role in lncRNA m6A methylation
Authors: Bei Mingrong , Hao Shijia , Lin Kai , Chen Qiuyang , Cai Yujie , Zhao Xing , Jiang Leiming , Lin Lirui , Dong Geng , Xu Jianzhen 
Published: June 2023

AbstractTransformer 2 alpha homolog (TRA2A), a member of the serine/arginine‐rich splicing factor family, has been shown to control mRNA splicing in development and cancers. However, it remains unclear whether TRA2A is involved in lncRNA regulation. In the present study, we found that TRA2A was upregulated and correlated with poor prognosis in esophageal cancer. Downregulation of TRA2A suppressed the tumor growth in xenograft nude mice. Epitranscriptomic microarray showed that depletion of TRA2A affected global lncRNA methylation similarly to the key m6A methyltransferase, METTL3, by silencing. MeRIP‐qPCR, RNA pull‐down, CLIP analyses, and stability assays indicated that ablation of TRA2A reduced m6A‐modification of the oncogenic lncRNA MALAT1, thus inducing structural alterations and reduced stability. Furthermore, Co‐IP experiments showed TRA2A directly interacted with METTL3 and RBMX, which also affected the writer KIAA1429 expression. Knockdown of TRA2A inhibited cell proliferation in a manner restored by RBMX/KIAA1429 overexpression. Clinically, MALAT1, RBMX, and KIAA1429 were prognostic factors of worse survival in ESCA patients. Structural similarity‐based virtual screening in FDA‐approved drugs repurposed nebivolol, a β1‐adrenergic receptor antagonist, as a potent compound to suppress the proliferation of esophageal cancer cells. Cellular thermal shift and RIP assay indicated that nebivolol may compete with MALAT1 to bind TRA2A. In conclusion, our study revealed the noncanonical function of TRA2A, which coordinates with multiple methylation proteins to promote oncogenic MALAT1 during ESCA carcinogenesis.
LncRNA‐HOTAIRM1 promotes aerobic glycolysis and proliferation in osteosarcoma via the miR‐664b‐3p/Rheb/mTOR pathway
Authors: Yu Xuecheng , Duan Weihao , Wu Furen , Yang Daibin , Wang Xin , Wu Jingbin , Zhou Dong , Shen Yifei 
Published: June 2023

AbstractOsteosarcoma (OS), which is a common and aggressive primary bone malignancy, occurs mainly in children and adolescent. Long noncoding RNAs (lncRNAs) are reported to play a pivotal role in various cancers. Here, we found that the lncRNA HOTAIRM1 is upregulated in OS cells and tissues. A set of functional experiments suggested that HOTAIRM1 knockdown attenuated the proliferation and stimulated the apoptosis of OS cells. A subsequent mechanistic study revealed that HOTAIRM1 functions as a competing endogenous RNA to elevate ras homologue enriched in brain (Rheb) expression by sponging miR‐664b‐3p. Immediately afterward, upregulated Rheb facilitates proliferation and suppresses apoptosis by promoting the mTOR pathway‐mediated Warburg effect in OS. In summary, our findings demonstrated that HOTAIRM1 promotes the proliferation and suppresses the apoptosis of OS cells by enhancing the Warburg effect via the miR‐664b‐3p/Rheb/mTOR axis. Understanding the underlying mechanisms and targeting the HOTAIRM1/miR‐664b‐3p/Rheb/mTOR axis are essential for OS clinical treatment.
Cardiorespiratory fitness components in relation to clinical characteristics, disease state and medication intake: A patient registry study
Authors: Cauwenberghs Nicholas , Sente Josephine , Sabovčik František , Ntalianis Evangelos , Hedman Kristofer , Claes Jomme , Goetschalckx Kaatje , Cornelissen Véronique , Kuznetsova Tatiana 
Published: June 2023

AbstractBackgroundInterpretation of cardiopulmonary exercise testing (CPET) results requires thorough understanding of test confounders such as anthropometrics, comorbidities and medication. Here, we comprehensively assessed the clinical determinants of cardiorespiratory fitness and its components in a heterogeneous patient sample.MethodsWe retrospectively collected medical and CPET data from 2320 patients (48.2% females) referred for cycle ergometry at the University Hospital Leuven, Belgium. We assessed clinical determinants of peak CPET indexes of cardiorespiratory fitness (CRF) and its hemodynamic and ventilatory components using stepwise regression and quantified multivariable‐adjusted differences in indexes between cases and references.ResultsLower peak load and peak O2 uptake were related to: higher age, female sex, lower body height and weight, and higher heart rate; to the intake of beta blockers, analgesics, thyroid hormone replacement and benzodiazepines; and to diabetes mellitus, chronic kidney disease, non‐ST elevation myocardial infarction and atrial fibrillation (p < 0.05 for all). Lower peak load also correlated with obstructive pulmonary diseases. Stepwise regression revealed associations of hemodynamic and ventilatory indexes (including heart rate, O2 pulse, systolic blood pressure and ventilation at peak exercise and ventilatory efficiency) with age, sex, body composition and aforementioned diseases and medications. Multivariable‐adjusted differences in CPET metrics between cases and controls confirmed the associations observed.ConclusionWe described known and novel associations of CRF components with demographics, anthropometrics, cardiometabolic and pulmonary diseases and medication intake in a large patient sample. The clinical implications of long‐term noncardiovascular drug intake for CPET results require further investigation.
Iran in the Sadrist version of Iraqi nationalism
Authors: Khanafira Mavadat Said 
Published: June 2023

AbstractUnlike many other Shiite political factions in Iraq, the Sadrist movement has become increasingly vocal in its criticism of Iranian influence in Iraq. Anti‐Iran chants have become almost ubiquitous in Sadrist demonstrations over the past few years, and prominent Sadrist politicians have unabashedly pointed the finger of blame for Iraq's crises at Iran. The growing Sadrist criticism of Iran has simultaneously come with the gradual ascendance of nationalist narratives in the religious movement. This article aims to study these narratives and their correlation with Iran. It argues that the Sadrist version of Iraqi nationalism encompasses three components that aim to differentiate Iraqi Arab Shiite identity from Iran. It also studies how Sadrist nationalism contributed to the rise of the Iran‐bashing trend among Iraqi Shiites.
Association of choline and betaine with the risk of cardiovascular disease and all‐cause mortality: Meta‐analysis
Authors: Yang Qinglin , Han Hua , Sun Zhongming , Liu Lu , Zheng Xingting , Meng Zeyu , Tao Na , Liu Jun 
Published: June 2023

AbstractBackgroundThis study aimed to systematically evaluate the role of circulating levels of choline and betaine in the risk of cardiovascular disease (CVD) and all‐cause mortality by comprehensively reviewing observational studies.MethodsThis study was conducted according to PRISMA 2020 statement. Six electronic databases, including PubMed, Embase and China National Knowledge Infrastructure (CNKI), were searched for cohort studies and derivative research design types (nested case–control and case‐cohort studies) from the date of inception to March 2022. We pooled relative risk (RR) and 95% confidence interval (CI) of the highest versus lowest category and per SD of circulating choline and betaine concentrations in relation to the risk of CVD and all‐cause mortality.ResultsIn the meta‐analysis, 17 studies with a total of 33,009 participants were included. Random‐effects model results showed that highest versus lowest quantile of circulating choline concentrations were associated with the risk of CVD (RR = 1.29, 95% CI: 1.04–1.61) and all‐cause mortality (RR = 1.62, 95% CI: 1.12–2.36). We also observed the risk of CVD were increased 13% (5%–22%) with per SD increment. Furthermore, highest versus lowest quantile of circulating betaine concentrations were not associated with the risk of CVD (RR = 1.07, 95% CI: 0.92–1.24) and all‐cause mortality (RR = 1.39, 95% CI: 0.96–2.01). However, the risk of CVD was increased 14% (5%–23%) with per SD increment.ConclusionsHigher levels of circulating choline were associated with a higher risk of CVD and all‐cause mortality.
Using digital technology to promote physical health in mental healthcare: A sequential mixed‐methods study of clinicians' views
Authors: Sawyer Chelsea , Hassan Lamiece , Sainsbury John , Carney Rebekah , Bucci Sandra , Burgess Harriet , Lovell Karina , Torous John , Firth Joseph 
Published: June 2023

AbstractAimRecent years have seen innovation in ‘mHealth’ tools and health apps for the management/promotion of physical health and fitness across the general population. However, there is limited research on how this could be applied to mental healthcare. Therefore, we examined mental healthcare professionals' current uses and perceived roles of digital lifestyle interventions for promoting healthy lifestyles, physical health and fitness in youth mental healthcare.MethodsA sequential, mixed‐methods design was used, consisting of a quantitative online survey, followed by qualitative in‐depth interviews.ResultsA total of 127 mental healthcare professionals participated in the online survey. Participants had limited mHealth experience, and the majority agreed that further training would be beneficial. Thirteen mental healthcare professionals were interviewed. Five themes were generated (i) digital technology's ability to enhance the physical healthcare; (ii) Conditions for the acceptability of apps; (iii) Limitations on staff capability and time; (iv) Motivation as the principal barrier; and (v) Practicalities around receiving lifestyle data. Systematic integration of data produced novel insights around: (i) staff involvement and needs; (ii) ideal focus and content of digital lifestyle interventions; and (iii) barriers towards implementation (including mental healthcare professionals own limited experience using digital lifestyle interventions, which aligned with the appeal of formal training).ConclusionsOverall, digital lifestyle interventions were positively received by mental healthcare professionals, particularly for health behaviour‐tracking and mHealth support for exercise and nutrition. Practical suggestions for facilitating their uptake/implementation to improve availability of physical health interventions in mental healthcare are presented.
Investigating causal relationships between genetically determined increased risk of attention‐deficit/hyperactivity disorder (ADHD) and atopic dermatitis (AD): A Mendelian randomization analysis
Authors: Ng W. , Loh M. , Yew Y. W. 
Published: June 2023

AbstractAtopic dermatitis (AD) is a chronic, inflammatory skin condition with a huge disease burden. Attention‐deficit/hyperactivity disorder (ADHD) is often diagnosed in children, and is associated with symptoms of inattention, hyperactivity and impulsive behaviour. Observational studies have demonstrated associations between AD and ADHD. However, to date, there has been no formal assessment of causal relationship between the two. We aim to evaluate causal relationships between genetically increased risk of AD and ADHD using Mendelian randomization (MR) approach. Two‐sample bi‐directional MR was conducted to elucidate potential causal relationships between genetically increased risk of AD and ADHD, using the largest and most recent genome‐wide association study datasets for AD and ADHD—EArly Genetics & Lifecourse Epidemiology AD consortium (21 399 cases and 95 464 controls) and Psychiatric Genomics Consortium (20 183 cases and 35 191 controls). Genetically determined increased risk of AD is not associated with ADHD based on genetic information: odds ratio (OR) of 1.02 (95% CI −0.93 to 1.11; p = 0.705). Similarly, genetic determined increased risk of ADHD is not associated with an increased risk of AD: OR of 0.90 (95% CI −0.76 to 1.07; p = 0.236). Horizontal pleiotropy was not observed from the MR‐Egger intercept test (p = 0.328) Current MR analysis showed no causal relationship between genetically increased risk of AD and ADHD in either direction in individuals of European descent. Any observed associations between AD and ADHD in previous population studies could possibly be due to confounding lifestyle factors such as psychosocial stress and sleeping habits.
An adaptive artificial electric field algorithm for continuous optimization problems
Authors: Chauhan Dikshit , Yadav Anupam 
Published: June 2023

AbstractThe comprehensive learning strategy is a meticulous method for enhancing the optimization ability of population‐based optimization algorithms. This article proposes an adaptive artificial electric field algorithm (iAEFA), which is developed by embedding a comprehensive learning strategy into AEFA. The proposed algorithm utilizes a novel adaptive approach for developing a better learning strategy in which an agent's velocity is updated using the comprehensive influence of the entire population. The developed scheme has shown a stronger potential to discover better candidate solutions in each iteration. The objective of the proposed method is to develop an efficient optimizer for continuous optimization problems. The performance of the proposed iAEFA is evaluated using a set of 13 classical benchmark test problems and the CEC 2019 (100‐digit challenge) benchmark functions. The experimental results are compared to seven state‐of‐the‐art optimization algorithms. Using the Wilcoxon signed‐rank test, the statistical significance of the results is confirmed. This article also discusses the theoretical convergence of the proposed algorithm, along with other significant findings about the proposed scheme. The experimental results and the theoretical analysis shows that the proposed scheme can be an excellent choice for the function optimization task compared to other existing algorithms.
FoxA2 is a reliable marker for the diagnosis of yolk sac tumour postpubertal‐type
Authors: Ricci Costantino , Ambrosi Francesca , Franceschini Tania , Giunchi Francesca , Di Filippo Giorgia , Franchini Eugenia , Massari Francesco , Mollica Veronica , Tateo Valentina , Bianchi Federico Mineo , Colecchia Maurizio , Acosta Andres Martin , Fiorentino Michelangelo 
Published: June 2023

AimsYolk sac tumour postpubertal‐type (YSTpt) shows a wide range of histological patterns and is challenging to diagnose. Recently, forkhead box transcription factor A2 (FoxA2) emerged as a driver of YSTpt formation and a promising marker for diagnosing YSTpt. However, FoxA2 has not been tested in the different patterns of YSTpt. This study aimed to assess the staining pattern of FoxA2 in te different patterns of YSTpt and other germ cell tumours of the testis (GCTT), comparing it with glypican‐3 (GPC3) and α‐fetoprotein (AFP).Methods and resultsFOXA2, GPC3 and AFP immunohistochemistry was performed on 24 YSTpt (24 microcystic/reticular, 10 myxoid, two macrocystic, five glandular/alveolar, two endodermal sinus/perivascular, four solid, two polyembryoma/embryoid body and two polyvesicular vitelline) and 81 other GCTT. The percentage of positive cells (0, 1+, 2+, 3+) and the intensity (0, 1, 2, 3) were evaluated regardless of and within each YSTpt pattern. FoxA2 was positive in all YSTpt (24 of 24) and all but one (23 of 24) exhibited 2+/3+ stain, with higher intensity [median value (mv): 2.6] than AFP (1.8) and GPC3 (2.5). Both FoxA2 and GPC3 were positive in all microcystic/reticular (24 of 24), myxoid (10 of 10), macrocystic (two of two), endodermal sinus/perivascular (four of four) and polyembryoma/embryoid body (two of two) patterns. Nevertheless, only FoxA2 was positive in all glandular/alveolar (five of five), solid (four of four) and polyvesicular vitelline (two of two) patterns. The intensity of FoxA2 was higher than AFP and GPC3 in almost all YST patterns. In the other GCTT, FoxA2 was positive only in teratoma postpubertal‐type (Tpt) [13 of 20 (65%)], with staining almost exclusively confined to the mature gastrointestinal/respiratory tract epithelium.ConclusionsFoxA2 is a highly sensitive and specific biomarker that supports the diagnosis of YSTpt. FoxA2 is superior to GPC3 and AFP, especially in rare and difficult‐to‐diagnose histological patterns of YSTpt, but mature glands of Tpt could represent a potential diagnostic pitfall.
Quantitative trait loci underlying a speciation phenotype
Authors: Waller Hayden , Blankers Thomas , Xu Mingzi , Shaw Kerry L. 
Published: June 2023

AbstractSexual signalling traits and their associated genetic components play a crucial role in the speciation process, as divergence in these traits can contribute to sexual isolation. Despite their importance, our understanding of the genetic basis of variable sexual signalling traits linked to speciation remains limited. In this study, we present new genetic evidence of Quantitative Trait Loci (QTL) underlying divergent sexual signalling behaviour, specifically pulse rate, in the Hawaiian cricket Laupala. By performing RNA sequencing on the brain and central nervous system of the parental species, we annotate these QTL regions and identify candidate genes associated with pulse rate. Our findings provide insights into the genetic processes driving reproductive isolation during speciation, with implications for understanding the mechanisms underlying species diversity.
Lost in data translation: A critical review of datasets on refugees
Authors: Sağnıç Şevin Gülfer 
Published: June 2023

AbstractQuantitative data are the backbone of the international refugee regime. Academic researchers and international organizations have been drawing attention to the problems of refugee data that obscure refugee vulnerabilities. Despite these efforts, the limitations of quantitative refugee data, particularly with regard to gendered aspects of refugee vulnerabilities, have often been overlooked. This article aims to address this issue by examining the major limitations of quantitative refugee data. It focuses on the question of “What are the major limitations of quantitative refugee data regarding gendered aspects of refugee vulnerabilities?” By conducting a systematic review of 67 datasets from international organizations, nation‐states, and independent data collectors, it argues that there are five fundamental problems with the data: (1) lack of disaggregation, (2) lack of cross‐tabulation and inability to harmonize datasets, (3) inconsistent periodization frequency, (4) lack of sex and gender diversity measurement, and (5) survivor bias. This article highlights the importance of critically analysing the data that informs theories and decisions regarding refugee vulnerabilities.
Preparation of isotropic submicron spherical boron nitride particles by restricted template method
Authors: Tian Zhaobo , Sun Siyuan , Du Songmo , Zhao Dengke , Zhang Jie , Li Fei , Chen Zhanglin , Zhu Yuan , Cui Wei , Liu Guanghua 
Published: June 2023

AbstractHexagonal boron nitride (h‐BN) has received considerable attention, due to its high thermal conductivity and electrical insulation. However, the intrinsic platelike structure with the strong anisotropic property restricts its applications, and it is necessary to synthesize isotropic spherical h‐BN particles (SP‐BNs) with submicron size. Till now, methods to prepare (SP‐BN) still exist problems, such as high oxygen impurities and pollution, generated by the ammonia and pyrolysis of precursors. Here, a relatively green reaction between the restricted template of carbon nanospheres and boron trioxide (B2O3) under elevated temperature is conducted, and the SP‐BNs with an average diameter of 200–300 nm (named Nano‐BN‐s) have been successfully synthesized. Comprehensive scanning electron microscopy, transmission electron microscopy, and X‐ray diffraction characterizations confirm the obtained products are spherical boron nitride. With the analysis of X‐ray photoelectron spectroscopy and Fourier transform infrared, the reaction mechanism is briefly discussed. These results indicate the reaction occurs on the restricted template of carbon nanospheres, and the C atoms are substituted by B and N atoms as the reaction progress, forming the Nano‐BN‐s. What is more, the restricted template method plays a key role in the design and improves h‐BN‐based materials in the future and may also be extended to form other novel materials.
Reputation is golden: Superstar CEOs and trade credit
Authors: Quan Xiaofeng , Xiang Cheng , (Tina) Gao Ru 
Published: June 2023

AbstractThis study investigates how Chief Executive Officer (CEO) reputation, proxied by receiving prestigious awards, impacts suppliers’ provision of trade credit to a firm. Employing a sample of Chinese public firms, we document that firms managed by award‐winning CEOs receive more trade credit than do propensity score matching matched control firms after the award year. Further analyses suggest that the increased trade credit of firms with reputable CEOs is due to the decreased default risk and information risk associated with those firms. Moreover, the impact of CEOs’ reputations on trade credit varies depending on award rarity, financing needs, CEO characteristics and firm opacity. Overall, our results are consistent with efficient contracting theory and signal theory.
Serum levels of vitamin D and periodontal inflammation in community‐dwelling older Japanese adults: The Otassha Study
Authors: Iwasaki Masanori , Motokawa Keiko , Shirobe Maki , Hayakawa Misato , Ohara Yuki , Motohashi Yoshiko , Edahiro Ayako , Kawai Hisashi , Fujiwara Yoshinori , Sakata Yasuyuki , Ihara Kazushige , Watanabe Yutaka , Obuchi Shuichi , Hirano Hirohiko 
Published: June 2023

AbstractAimTo evaluate the association between vitamin D status and periodontal inflammation as determined by the periodontal inflamed surface area (PISA) in community‐dwelling older adults.Materials and MethodsThis cross‐sectional study included 467 Japanese adults (mean age = 73.1 years) who underwent full‐mouth periodontal examinations and measurements of serum levels of 25‐hydroxyvitamin D (25(OH)D). We used linear regression and restricted cubic spline models to analyse the association between exposure (serum 25(OH)D) and outcome (PISA).ResultsThe linear regression model showed that, after adjusting for potential confounders, participants in the lowest quartile of serum 25(OH)D had 41.0 mm2 more PISA (95% confidence interval [CI]: 4.6–77.5) than the reference group (the highest quartile of serum 25(OH)D). The spline model showed that the association between serum 25(OH)D and PISA was non‐linear and restricted to the low 25(OH)D range. PISA initially sharply decreased as serum 25(OH)D increased, and then the decreasing trend slowed and plateaued. The inflection point with the minimum PISA value was a serum 25(OH)D level of 27.1 ng/mL, above which there was no decreasing trend in PISA with increasing serum 25(OH)D levels.ConclusionsLow vitamin D status had an L‐shaped association with periodontal inflammation in this cohort of Japanese adults.