bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of catechol in tea based on the inhibition of CS@Cd composites electrochemiluminescence
Authors: Liu Gen , Yao Chunyu , Zhang Hui , Ma Wei 
Published: February 2023

Abstract

To address growing concerns about food safety, sensors for catechol (CA) have been urgently needed. In this work, cadmium-supported carbon spheres (CS@Cd) composites are prepared via hydrothermal synthesis and further used to fabricate a CS@Cd modified glassy carbon electrode (CS@Cd/GCE). Importantly, CS@Cd has good sensitization effect on the electrochemiluminescence (ECL) of luminol-H2O2 system. Besides, CA is able to inhibit the ECL of CS@Cd/GCE owing to the consumption of H2O2 induced by CA, and thus a novel strategy for ECL detection of CA is formulated. At optimum conditions, CS@Cd/GCE exhibits excellent linear relationship in the range of 1.0×10-11 ~ 1.0×10-4 mol·L-1 for CA detection with a low limit of detection of 2.5×10-12 (S/N = 3). Finally, this method achieves a satisfactory outcome for the detection of CA in tea samples.
Productivity and profitability of small-scale aquaculture in Malawi
Authors: Munthali Maggie Golie , Chilora Lemekezani , Wineman Ayala , Nankwenya Bonface , Chiwaula Levison , Muyanga Milu 
Published: February 2023

Abstract

Small-scale aquaculture in Malawi is thought to contribute to nutrition and food security and serve as an income diversification strategy. Nevertheless, its adoption is low. Drawing on a recent survey of 734 small-scale fish farms across the country, the present study assesses the productivity and profitability of small-scale fish farms, and their determinants using regression analysis. Most fish farms are owned and managed by individual farm-households, though communally owned farms are also present. Small-scale aquaculture is found to be profitable, though the gross margins are slim. Regression results reveal that productivity and profitability are positively affected by the use of farms for both fingerling and grow-out production; the use of high-quality inputs such as commercial feed and inorganic fertilizers; and number of years the farm has existed. These results confirm that small-scale fish farmers in Malawi and other similar settings should adopt improved technologies and practice best on-farm management practices to increase productivity and profitability. This study contributes to the discourse regarding the pathway through which aquaculture in Malawi can achieve its potential to contribute to the country’s development.
Tracheal Reconstruction Surgery for Congenital Tracheal Stenosis
Authors: Tanh Nguyen Tran Viet , Thach Pham Ngoc , Phong Le Si , Ngoc Le Thi Duyen , Nguyen Phan Thao , Dung Ho Van Anh , Mau Truong Anh , Nhan Vu Truong 
Published: February 2023

Abstract
Purpose
Congenital tracheal stenosis is a rare but dangerous disease. Reconstructive tracheal surgery is a life-saving treatment but also a challenging procedure. This study aims to evaluate the outcomes of tracheal reconstruction surgery.
Methods
A prospective cohort study was conducted with all the records of congenital tracheal stenosis which had been managed by tracheal reconstruction surgery at Children’s Hospital 2 Ho Chi Minh city from August 2013 to August 2022.
Results
67 cases, who underwent slide tracheoplasty, were included in our study. Mean age was 7.6 months (25 days – 8 years). Common congenital associated lesion was left pulmonary artery sling, accounting for 65,7% of cases. Bronchial stenosis was found in 22.4% patients. Emergency surgery was performed in 8 cases. The survival rate in this review was 86.6%. Nine patients died in which 4/9 case (44.4%) were emergency surgery. The recurrent stenosis rate was 8.9%, only 2 cases needed reoperation in which 1 died and 1 recovered uneventfully. The outcomes of surgery were good in 53 cases (79,1%).
Conclusion
Tracheal reconstruction surgery with slide tracheoplasty technique is safe and versatile technique which is feasible in every case of congenital tracheal stenosis. Mortality was associated with severe cases which required emergency surgery.
A Two-field Approach to Multibody Dynamics of Rotating Flexible Bodies
Authors: Géradin Michel M. , Sonneville Valentin 
Published: February 2023

Abstract
A two-field version of the floating frame of reference approach is developed to describe the dynamics of flexible body in the case of motion in a uniformly rotating frame. Full decoupling of the kinetic energy results from splitting of the motion into rigid body modes of the floating center of mass and a velocity field orthogonal to them. The motion equations are developed from a canonical variational expression in which displacement and velocity fields play independent roles. They turn out to keep the same global structure as in the case of motion in an inertial frame. The discretization of all inertia terms is discussed in depth. It is also shown that, in the case of discretization of the continuum with 3D solid elements, all gyroscopic terms can be derived from a mass kernel and associated Si matrices. Model reduction is proposed using the Herting-Martinez method in order to automatically satisfy orthogonality of the velocity field to rigid body modes. Two application examples of high-speed rotating systems are developed in length to assess the efficiency of the proposed methodology.
Cyclopeptide self-assembly simulated epidemic sequential and synchronous complexity
Authors: Xia Zhenfang , Wang Yong , Wang Qi 
Published: February 2023

Abstract
Decoding the sequential and synchronous complexities of epidemic outbreaks will help guide the scientific response to the epidemic. Here, the complexity of epidemic sequential and synchronous was simulated from the perspective and method of cyclopeptide self-assembly, and the process of cyclopeptide self-assembly was observed by molecular fluorescence and morphological changes, reflecting the characteristics of the epidemic changes. The results showed that the cyclopeptide, namely cyclo(FWWYYF), self-assembly process took different forms under different concentrations and solvents. At lower concentrations, cyclopeptide molecules simulated the complexity of epidemic sequential and synchronous, while at higher concentrations, cyclopeptide molecules self-assembly also behaves as a non-sequential and non-synchronous composite multimodal model. These results indicated that the complexity of the epidemic outbreaks was not only the complexity of sequential and synchronous, but also the emergence of non-sequential and non-synchronous complex multimodal models. Molecular simulations elucidated why the global pandemic required global solidarity and synchronization.
Effect of Irregularity and Inhomogeneity on Love wave Propagation in Dry Sandy Layer Bounded by Monoclinic Elastic Layer and Isotropic Elastic Half-space
Authors: Kumar Naveen , Madan Dinesh Kumar 
Published: February 2023

Abstract
The present study deals with the Love wave propagation in a dry sandy layer bounded by an upper monoclinic elastic layer and a lower inhomogeneous isotropic elastic half-space. Rectangular-type irregularity is considered at the interface of sandy layer and half-space. Analytical expressions for displacement fields are derived by adopting variable separation method. The dispersion frequency equation for the phase velocity of Love wave is also derived by using suitable boundary conditions. Special cases for orthotropic elastic half-space is discussed. Also the validation with standard Love wave equation is also verified, discussed in the particular case. Significant effects of various parameters like sandiness, irregularity, inhomogeneity and thickness ratio are observed by analyzing the dispersion frequency equation graphically using the MATLAB software. Theoretical results obtained regarding Love wave propagation in the considered model serve as major highlights, having significant relevance in the field of geophysics, materials physics, soil mechanics and earthquake engineering.
Molecular subtypes based on PANoptosis-related genes and tumor microenvironment infiltration characteristics in Lower-grade glioma
Authors: Abulaiti Aimitaji , Maimaiti Aierpati , Yiming Nadire , Fu Qiang , Li Shaoshan , Li Yabin , Wang Yongxin , Zhou Qingjiu 
Published: February 2023

Abstract
The growth of cancer, the effectiveness of treatment, and prognosis are all closely related to PANoptosis (include pyroptosis, apoptosis, and necroptosis). It remains unclear whether PANoptosis genes (PANGs) may contribute to lower-grade glioma tumor microenvironment (TME). In this study, Firstly, we provided a comprehensive review of the pan-cancer landscape of PANGs in terms of expression characteristics, prognostic value, mutational profile, and pathway regulation. Then, we identified two distinct PAN clusters in 611 LGG patients, each with its own molecular, clinical, and immunological profile. We then developed a scoring system for LGG patients called PANscore. As well as investigating immune characteristics, tumor mutational characteristics, and drug sensitivity, we examined the differences between groups with high-PANscores and those with low-PANscores. Based on this PANscore and a number of clinicopathological variables, an instant nomogram for predicting clinical survival in LGG patients was developed. Our thorough examination of PANGs in LGG revealed their probable function in TME, as well as their clinicopathological characteristics and prognosis. These discoveries could deepen our comprehension of PANGs in LGG and provide doctors fresh perspectives on how to forecast prognosis and create more efficient, individualized treatment plans.
Integration of clinical and spatial data to explore lipid metabolism-related gene for predicting prognosis and immune microenvironment in gliomas
Authors: Yu Zhangyi , Zhou Yuneng , Li Yongxue , Dong Zhiqiang 
Published: February 2023

Abstract
Lipid metabolism is crucial to tumor growth and immune microenvironment as well as drug sensitivity in glioma. Identifying prognostic indicators of glioma and elucidating the mechanisms of glioma progression is for improving glioma patient prognosis. In this study, we investigated the role and prognostic value of metabolism-related genes in glioma by GEO, CGGA, and TCGA. Based on clinical data and transcriptome data, We found that the expression pattern of three major pathways of lipid metabolism is fatty acidhigh-phospholipidhigh-triglyceridelow, which is associated with better prognosis and immune infiltration. Using the related genes of these three pathways constructed a prognostic model, and the model showed stability and efficiency in the test set and validation set. In the spatial transcriptome of glioma patients, the microenvironment of the regions with high expression of risk gene CAV1 and SCD is in a state of hypoxia, EMT, and cell cycle arrest, and thus can be used as markers of metabolic reprogramming in the tumor microenvironment. In the high-risk group, M0 macrophages and M1 macrophages were significantly enriched, and the risk score was significantly correlated with gene mutation and methylation. screened the sensitive drugs corresponding to different risk genes. This study provided novel insights into the differential immune microenvironment with different metabolic expression patterns and highlighted the spatial and temporal synergy of tumor progression and metabolic reprogramming.
Prevalence, Patterns and Associated Factors of Acute Tibia Plateau Fractures among Adults with Tibia Fractures in Uganda
Authors: Mfaume Benjamin Costantine , Mtisi Expeditho , Sali Patric , Sheikdon Abdirizak , Michael Emusugut , Gonzaga Waiswa , Bugeza Samuel , Mutanda Tonny 
Published: February 2023

Abstract
Background: The Tibia plateau makes up one of the most critical load bearing articular surface in the human body. An injury to this vital anatomic region affects knee alignment, stability and ultimately impairs functional mobility. Tibia Plateau fractures are associated with poor outcomes and more significant late complications if not well managed.
Objective: To determine the prevalence, patterns and associated factors of Tibia Plateau fractures (TPFs) among adults with tibia fractures seen at Mulago National Referral Hospital using Computerized tomography.
Methods: This was a cross sectional study which assessed tibia plateau fractures using computerized tomography. The prevalence of tibia plateau fractures was determined as a proportion of tibia plateau fractures among all tibia fractures enrolled in the study. The patterns were determined as a proportion of a particular pattern among tibia plateau fractures. For factors associated with TPFs, we performed a Fisher’s exact test and factors with P-values less than 0.2 at bivariate were considered to have an independent association with tibia plateau fractures
Results: 283 participants with tibia fractures were enrolled, 40 had tibia plateau fractures resulting in a prevalence of 14.1% (95% CI; 10.5 to 18.7). In this study 75% of the injuries involved the right tibia and 57.5% were closed injuries. Majority of tibia plateau fractures were classified as Schatzker VI at 60.0%. The most common column classification was medial accounting for 40% while the lateral and posterior Columns were equally distributed by 30.0% each. Sex of participant was found to have an independent association with TPFs
Conclusion: Tibia plateau fractures are common injuries with a high prevalence in our setting. Road traffic accidents were the most common mechanism of injury affecting mainly motorcyclists and pedestrians. Shatzker VI and medial column is the most common fracture pattern in our setting.
An exploration of market organic sanitary products for improving menstrual health and environmental impact
Authors: Hand Jamie , Hwang Christina , Vogel Wren , Lopez Christina , Hwang Sangchul 
Published: February 2023

AbstractMany menstruators around the world have limited access to education about menstruation and to sanitary methods for managing menstruation. The use of sanitary products is influenced by economic status, proximity to resources, education, and cultural beliefs. Improper use of sanitary products or lack thereof can lead to major health issues. Plastic-based, commercial pads take decades to degrade and cause harm to the environment and pose health risks to menstruators. To combat the lack of education and accessibility to menstrual products, there is a need to develop inexpensive, environmentally friendly, and culturally acceptable menstrual hygiene pads. Organic sanitary pads have been developed with locally available materials, such as water hyacinth, banana stems, bamboo, papyrus, hemp, and cotton. These sanitary pads have the potential to create better health and economic outcomes for menstruators across the globe and reduce the environmental footprint.
Disruption of Staphylococcus aureus Biofilms with Purified Moringa
oleifera Leaf Extract Protein
Authors: Biswas Sumit , Menon Lakshmi , Chouhan Omprakash , Walke Rushikesh , Shah Shruti , Damare Samir 
Published: February 2023

Background:
A major cause of economic losses in the medical implant sector has been
bacterial biofilms due to their ability to persist on various surfaces and their tolerance against
endogenous defences, antibiotics, or other anti-microbial agents. The quest for potential sources
causing inhibition or disruption of bacterial biofilms has been taken up to alleviate the loss. Plantderived
extracts such as essential oils, bioactive compounds and other solvent extracts are regularly
being used instead of antibiotics and other synthetic compounds as they are safer, economical, and in
many instances, have an elaborate history of traditional medicinal usage.

Objectives:
As a plant that has been traditionally used over the centuries, the Moringa oleifera Lam.,
or more commonly the drumstick tree, is being tapped for myriad pharmaceutical applications. The
protein-rich leaf of this tree has not only proved to be of great nutritional value but also divulged
numerous potential therapeutic applications.

Methods:
While reports of proteinaceous components are rare, here we report the efficacy of the
aqueous extract of the leaf of M. oleifera and a 62 kDa protein component in the disruption of
staphylococcal biofilms, which are largely implicated in nosocomial infections.

Results:
The application of the M. oleifera leaf extract protein had a marked effect on the biofilm
growth or formation by Staphylococcus aureus.

Conclusion:
While the crude extract itself showed considerable disruption of biofilm formation, the
application of the purified protein obtained after a two-step process led to a significant increase in the
anti-biofilm activity.
The Chemical Synthesis of the 1-C-Alkyl Substituted Pyrrolidine and Piperidine
Iminosugar Natural Products and their Analogues
Authors: Byatt Brendan J. , Pyne Stephen G. 
Published: February 2023

Abstract:
A review of the chemical synthesis of the 1-C-alkyl substituted pyrrolidine and
piperidine iminosugar natural products and their analogues (where the alkyl chain comprises
two or more carbons) is provided. These syntheses can be grouped into nine different synthetic strategies that share a common approach toward installing the alkyl substituent. These include nucleophilic addition to aldimines; Grignard additions to glycosylamines, cyclic imides,
and carbohydrates; Weinreb ketone synthesis; nucleophilic addition to cyclic nitrones; the
Overmann rearrangement; the allylation of hemiaminals; and the Petasis borono-Mannich
reaction. The broussonetine alkaloids have proven popular synthetic targets to develop new
synthetic methods and verify these target molecules' structures and stereochemistry.
Study on the Mechanism of Dachaihu Decoction in the Treatment of Acute
Pancreatitis Based on Artificial Intelligence Combined with in vivo Experiments
Authors: Zhang Yan , Li Sujie , Zhang Xiujiang , Wang Pei , Meng Zhe , Pang Yuming , Li Wei 
Published: February 2023

Background and Aim:
To explore the possible mechanism of Dachaihu Decoction
(DCHD) in the treatment of AP, and use in vivo experiments to verify.

Methods:
The targets and active ingredients of DCHD in the treatment of AP were obtained
through network pharmacology, and the preliminary verification was carried out by molecular
docking. Caerulein was used to develop the AP rat model. H&E staining was performed to observe
variations in pancreatic tissue. Western blot and RT-qPCR were conducted to evaluate the associated
proteins and mRNA.

Results:
The network pharmacology and molecular docking results showed that the key targets
(EGFR, TNF, SRC, VEGFA and CTNNB1) and key active components (beta-sitosterol, stigmasterol,
baicalein, quercetin, and kaempferol) of DCHD in the treatment of AP had good binding.
H&E staining revealed that rat pancreatic tissues considerably damaged post caerulein intervention,
and it has also been suggested that DCHD ameliorates damage to pancreatic tissue. Simultaneously,
EGFR, TNF, SRC, VEGFA protein, and mRNA expression levels were increased in the
model group compared to the blank group (P < 0.01), whereas CTNNB1 expression was found to
be decreased in the model group (P < 0.01). Compared with the model group, the protein expression
levels of EGFR, TNF, SRC, and VEGFA in the treatment group were down-regulated (P <
0.01), and CTNNB1 was up-regulated (P < 0.05).

Conclusion:
DCHD protects pancreatic tissues and improves symptoms in AP rats by upregulating
CTNNB1 protein and mRNA while inhibiting EGFR, TNF, SRC, and VEGFA protein and mRNA
expression.
Influence of SLCO1B1 Polymorphisms on the Pharmacokinetics of Mycophenolic
Acid in Renal Transplant Recipients
Authors: Liu Jiawen , Tan Ruoyun , Gu Min , Wang Zijie , Zhu Yongqian , Zhang Jiexiu , Wei Jintao , Zheng Ming , Gui Zeping , Chen Hao , Sun Li , Han Zhijian , Tao Jun , Ju Xiaobin 
Published: February 2023

Aims:
This study was designed to analyze the correlation between single nucleotide polymorphisms
(SNP) related to drug metabolism and pharmacokinetics of mycophenolic acid (MPA) during long-term follow-up.

Materials and Method:
A retrospective cohort study involving 71 renal transplant recipients was designed. Blood
samples were collected to extract total DNAs, followed by target sequencing based on next-generation sequencing
technology. The MPA area under the curve (AUC) was calculated according to the formula established in our center.
The general linear model and linear regression model were used to analyze the association between SNPs and MPA
AUC.

Results:
A total of 689 SNPs were detected in our study, and 90 tagger SNPs were selected after quality control and
linkage disequilibrium analysis. The general linear model analysis showed that 9 SNPs significantly influenced MPA
AUC. A forward linear regression was conducted, and the model with the highest identical degree (r2=0.55) included
4 SNPs (SLCO1B1: rs4149036 [P < 0.0001], ABCC2: rs3824610 [P = 0.005], POR: rs4732514 [P = 0.006], ABCC2:
rs4148395 [P = 0.007]) and 6 clinical factors (age [P < 0.0001], gender [P < 0.0001], the incident of acute rejection
(AR) [P = 0.001], albumin [P < 0.0001], duration after renal transplantation [P = 0.01], lymphocyte numbers [P =
0.026]). The most relevant SNP to MPA AUC in this model was rs4149036. The subgroup analysis showed that
rs4149036 had a significant influence on MPA AUC in the older group (P = 0.02), high-albumin group (P = 0.01),
male group (P = 0.046), and both within-36-month group (P = 0.029) and after-36-month group (P = 0.041). The
systematic review included 4 studies, and 2 of them showed that the mutation in SLCO1B1 resulted in lower MPA
AUC, which was contrary to our study.

Conclusion:
A total of 4 SNPs (rs4149036, rs3824610, rs4148395, and rs4732514) were identified to be significantly
correlated with MPA AUC. Rs4149036, located in SLCO1B1, was suggested to be the most relevant SNP to MPA
AUC, which had a stronger influence on recipients who were elder, male, or with high serum albumin. Furthermore,
6 clinical factors, including age, gender, occurrence of acute rejection, serum albumin, time from kidney transplantation,
and blood lymphocyte numbers, were found to affect the concentration of MPA.
Comprehensive Study of In vivo and In vitro Metabolites of Cycloastragenol Based on
UHPLC-Q-Exactive Orbitrap Mass Spectrometer
Authors: Li Huajian , Wang Shaoping , Wang Hong , Li Haoran , Li Yanan , Dong Pingping , Lan Xianming , Zhang Jiayu , Dai Long 
Published: February 2023

Background:
Cycloastragenol (CAG) is a sapogenin derived from the main bioactive constituents of
Astragali Radix (AR). However, the current research on CAG metabolism in vivo and in vitro is still inadequate, and
the metabolite cluster is incomplete due to incomplete analysis strategy.

Objective:
The objective of this study was to screen and identify the metabolic behavior of CAG in vivo and in vitro.

Methods:
A simple and rapid analysis strategy based on UHPLC-Q-Exactive Orbitrap mass spectrometry combined
with data-mining processing technology was developed and used to screen and identify CAG metabolites in rat body
fluids and tissues after oral administration.

Results:
As a result, a total of 82 metabolites were fully or partially characterized based on their accurate mass, characteristic
fragment ions, retention times, corresponding Clog P values, and so on. Among the metabolites, 61 were
not been reported in previous reports. These metabolites (6 metabolites in vitro and 91 in vivo) were generated
through reactions of hydroxylation, glucuronidation, sulfation, hydrogenation, hydroxylation, demethylation,
deisopropylation, dehydroxylation, ring cleavage, and carboxyl substitution and their composite reactions, and the
hydroxylation might be the main metabolic reaction of CAG. In addition, the characteristic fragmentation pathways
of CAG were summarized for the subsequent metabolite identification.

Conclusion:
The current study not only clarifies the metabolite cluster-based and metabolic regularity of CAG in
vivo and in vitro, but also provides ideas for metabolism of other saponin compounds.