bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemodiversity in flowers ofTanacetum vulgarehas consequences on a florivorous beetle
Authors: Sasidharan Rohit , Brokate Lukas , Eilers Elisabeth J. , Müller Caroline 
Published: June 2023

ABSTRACTThe chemical composition of plant individuals can vary, leading to high intraspecific chemodiversity. Diversity of floral chemistry may impact the responses of flower-feeding insect visitors.Plants ofTanacetum vulgarevary significantly in their leaf terpenoid composition, resulting in distinct chemotypes. We investigated the composition of terpenoids and nutritents of flower heads and pollen in plants belonging to three chemotypes, dominated either by β-thujone (BThu), artemisia ketone (Keto) or a mixture of (Z)-myroxide, santolina triene and artemisyl acetate (Myrox). Moreover, we tested the effects of these differences on preferences, weight gain and performance of adults of the shining flower beetle,Olibrus aeneus.The terpenoid composition and diversity of flower heads and pollen significantly differed among individuals belonging to these chemotypes, while total concentrations of terpenoids, sugars, amino acids or lipids of the pollen did not differ. Beetles preferred the BThu over the Myrox chemotype in both olfactory and contact choice assays, while the Keto chemotype was marginally repellent in olfactory assays. The beetles gained the least weight within 48 h and their initial mortality was highest when feeding exclusively on floral tissues of the Myrox chemotype. Short-term weight gain and long-term performance were highest on the BThu chemotype.In conclusion, the beetles showed chemotype-specific responses towards differentT. vulgarechemotypes, which may be attributed to the terpenoid composition in flower heads and pollen rather than to differences in nutrient profiles. Both richness and overall diversity are important factors when determining chemodiversity of individual plants and their consequences on interacting insects.SHORT SUMMARYWe demonstrate thatTanacetum vulgarechemotypes distinguished by their leaf terpenoid profiles also show unique floral and pollen chemotypes based on terpenoid composition and diversity, which affect the preference and performance of a beetle florivore.
Comparison of control and transmission of COVID-19 across epidemic waves in Hong Kong: an observational study
Authors: Yang Bingyi , Lin Yun , Xiong Weijia , Liu Chang , Gao Huizhi , Ho Faith , Zhou Jiayi , Zhang Ru , Wong Jessica Y. , Cheung Justin K. , Lau Eric H. Y. , Tsang Tim K. , Xiao Jingyi , Wong Irene O. L. , Martín-Sánchez Mario , Leung Gabriel M. , Cowling Benjamin J. , Wu Peng 
Published: June 2023

ABSTRACTBackgroundHong Kong contained COVID-19 for two years, but experienced a large epidemic of Omicron BA.2 in early 2022 and endemic transmission of Omicron subvariants thereafter.MethodsWe examined the use and impact of pandemic controls in Hong Kong by analysing data on more than 1.7 million confirmed COVID-19 cases and characterizing non-pharmaceutical and pharmaceutical interventions implemented from January 2020 through to 30 December 2022. We estimated the daily effective reproductive number (Rt) to track changes in transmissibility and effectiveness of community-based measures against infection over time. We examined the temporal changes of pharmaceutical interventions, mortality rate and case-fatality risks (CFRs), particularly among older adults.FindingsHong Kong experienced four local epidemic waves predominated by the ancestral strain in 2020 and early 2021 and prevented multiple SARS-CoV-2 variants from spreading in the community before 2022. Strict travel-related, case-based, and community-based measures were increasingly tightened in Hong Kong over the first two years of the pandemic. However, even very stringent measures were unable to contain the spread of Omicron BA.2 in Hong Kong. Despite high overall vaccination uptake (>70% with at least two doses), high mortality was observed during the Omicron BA.2 wave due to lower vaccine coverage (42%) among adults ≥65 years of age. Increases in antiviral usage and vaccination uptake over time through 2022 was associated with decreased case fatality risks.InterpretationIntegrated strict measures were able to reduce importation risks and interrupt local transmission to contain COVID-19 transmission and disease burden while awaiting vaccine development and rollout. Increasing coverage of pharmaceutical interventions among high-risk groups reduced infection-related mortality and mitigated the adverse health impact of the pandemic.
HTLV-1 Rex hijacks UPF1 in a CRM1 dependent manner, leading to NMD inhibition and revealing unexpected proviral roles of UPF1
Authors: Prochasson L , Mghezzi-Habellah M , Roisin A , Palma M , Robin JP , de Bossoreille S , Sareoua L , Cluet D , Mouehli M , Decimo D , Réty S , Desrames A , Chaze T , Matondo M , Dutartre H , Thoulouze MI , Lejeune F , Jalinot P , Mocquet V 
Published: June 2023

AbstractThe hijacking of CRM1 export is an important step of the retroviral replication cycle. Here, we investigated the consequences of this hijacking for the host. During HTLV-1 infection, we identified the formation of a complex composed of Rex, CRM1 and the RNA helicase UPF1, leading to the nuclear retention of UPF1. We further showed how this leads to the inhibition of the nonsense mediated mRNA decay (NMD), known to have an antiviral function. Corroborating these results, we described a similar process with Rev, the functional homolog of Rex from HIV-1. Unexpectedly, we also found that, for HTLV-1, this process is coupled with the specific loading of UPF1 onto vRNA, independently of NMD. In this latter context, UPF1 positively regulates several steps of the viral replication cycle, from the nuclear export of vRNA to the production of mature viral particles.
Virome analysis of irrigation water sources provides extensive insights into the diversity and distribution of plant viruses in agroecosystems
Authors: Maksimović Olivera , Bačnik Katarina , Selda Rivarez Mark Paul , Vučurović Ana , Mehle Nataša , Ravnikar Maja , Gutiérrez-Aguirre Ion , Kutnjak Denis 
Published: June 2023

AbstractPlant viruses pose a significant threat to agriculture. Several are stable outside their hosts, can enter water bodies and remain infective for prolonged periods of time. Even though the quality of irrigation water is of increasing importance in the context of plant health, the presence of plant viruses in irrigation waters is understudied. In this study, we conducted a large-scale high-throughput sequencing (HTS)-based virome analysis of irrigation and groundwater sources to obtain complete information about the abundance and diversity of plant viruses in such waters. We detected nucleic acids of plant viruses from 20 families, discovered several novel plant viruses from economically important taxa, likeTobamovirusand observed the influence of the water source on the present virome. By comparing viromes of water and surrounding plants, we observed presence of plant viruses in both compartments, especially in cases of large-scale outbreaks, such as that of tomato mosaic virus. Moreover, we demonstrated that water virome data can extensively inform us about the distribution and diversity of plant viruses for which only limited information is available from plants. Overall, the results of the study provided extensive insights into the virome of irrigation waters from the perspective of plant health. It also suggested that an HTS-based water virome surveillance system could be used to detect potential plant disease outbreaks and to survey the distribution and diversity of plant viruses in the ecosystems.
A Likelihood Ratio Test for Hybridization Under the Multispecies Coalescent
Authors: Peng Jing , Kong Sungsik , Kubatko Laura 
Published: June 2023

AbstractSeveral methods have been developed to carry out a statistical test for hybridization at the species level, including the ABBA-BABA test andHyDe. Here, we propose a new method for detecting hybridization and quantifying the extent of hybridization. Our test computes the likelihood of a species tree that is possibly subject to hybridization using site pattern frequencies from genomic-scale datasets under the multispecies coalescent. To do this, we extend the calculation of the likelihood for site pattern frequency data for the 4-taxon symmetric and asymmetric species trees proposed in Chifman and Kubatko (2015) by incorporating an inheritance parameter, resulting in efficient computation of the likelihood under a scenario of hybridization. We use this likelihood computation to construct a likelihood ratio test that a given species is a hybrid of two parental species. Simulations demonstrate that our test is more powerful than existing tests of hybridization, includingHyDe, and that it achieves the desired type I error rate. We apply the method to two empirical data sets, one for which hybridization is believed to have occurred and one for which previous methods have failed to detect hybridization.
Analysis of DNA methylation in rainbow trout spermatozoa: the strengths and limitations of RRBS
Authors: Marina El Kamouh , Aurélien Brionne , Amin Sayyari , Delphine Lallias , Catherine Labbé , Audrey Laurent 
Published: June 2023

AbstractDNA methylation is an important epigenetic mark in fish spermatozoa since it has been shown that some sperm methylome features are transmitted to the offspring. To ensure the transmission of unaltered information to the offspring, the characterization of this mark and its stability in spermatozoa is essential. DNA methylation status can be assessed at the whole genome level with an identification of the methylated and unmethylated cytosines using RRBS (reduced representation bisulfite sequencing). This method allows the sequencing of a subset of the genome expected to be enriched in CpGs. We aim to characterize the data provided by RRBS in rainbow trout spermatozoa, in order to evaluate the suitability of this approach for sperm biotechnologies studies. We observed that RRBS did provide a reduced amount of genomic data, thus allowing the processing of many biological replicates. Although, in our dataset, only a small fraction of the whole genome CpGs was present in all 6 to 12 replicates, the sum of the analyzed CpGs spanned 9 % of the total genomic CpGs. They distributed evenly all over the genome, and all genomic features were represented. RRBS is therefore an effective method to scan the DNA methylation of the along genome in a reduced pattern. However, one should be aware of the choices that are to be made regarding fragment size selection and regarding the options during bioinformatic data processing.
Opposing chemosensory functions of closely related gustatory receptors
Authors: Ahn Ji-Eun , Amrein Hubert 
Published: June 2023

ABSTRACTMost animals have functionally distinct populations of taste cells, expressing receptors that are tuned to compounds of different valence. This organizational feature allows for discrimination between chemicals associated with specific taste modalities and facilitates differentiating between unadulterated foods and foods contaminated with toxic substances. In the fruit flyD. melanogaster, primary sensory neurons express taste receptors that are tuned to distinct groups of chemicals, thereby activating neural ensembles that elicit either feeding or avoidance behavior. Members of a family of ligand gated receptor channels, the Gustatory receptors (Grs), play a central role in these behaviors. In general, closely related, evolutionarily conserved Gr proteins are co-expressed in the same type of taste neurons, tuned to chemically related compounds, and therefore triggering the same behavioral response. Here, we report that members of the Gr28 subfamily are expressed in largely non-overlapping sets of taste neurons inDrosophilalarvae, detect chemicals of different valence and trigger opposing feeding behaviors. We determined the intrinsic properties ofGr28neurons by expressing the mammalian Vanilloid Receptor (VR1), which is activated by capsaicin, a chemical to which wildtypeDrosophilalarvae do not respond. When VR1 is expressed inGr28aneurons, larvae become attracted to capsaicin, consistent with reports showing thatGr28aitself encodes a receptor for nutritious RNA. In contrast, expression of VR1 in two pairs ofGr28b.cneurons triggers avoidance to capsaicin. Moreover, neuronal inactivation experiments show that theGr28b.cneurons are necessary for avoidance of several bitter compounds. Lastly, behavioral experiments ofGr28deficient larvae and live Ca2+imaging studies ofGr28b.cneurons revealed that denatonium benzoate, a synthetic bitter compound that shares structural similarities with natural bitter chemicals, is a ligand for a receptor complex containing a Gr28b.c or Gr28b.a subunit. Thus, theGr28proteins, which have been evolutionarily conserved over 260 million years in insects, represent the first taste receptor subfamily in which specific members mediate behavior with opposite valence.
Blockade of IKK signaling induces RIPK1-independent apoptosis in human cells
Authors: Nataraj Neha M. , Herrmann Beatrice , Shin Sunny , Brodsky Igor E. 
Published: June 2023

ABSTRACTRegulated cell death in response to microbial infection plays an important role in immune defense and is triggered by pathogen disruption of essential cellular pathways. Gramnegative bacterial pathogens in theYersiniagenus disrupt NF-κB signaling via translocated effectors injected by a type III secretion system (T3SS), thereby preventing induction of cytokine production and antimicrobial defense. In murine models of infection,Yersiniablockade of NF-κB signaling triggers cell-extrinsic apoptosis through Receptor Interacting Serine-Threonine Protein Kinase 1 (RIPK1) and caspase-8, which is required for bacterial clearance and host survival. Unexpectedly, we find that human macrophages undergo apoptosis independently of RIPK1 in response toYersiniaor chemical blockade of IKKα/β. Instead, IKK blockade led to decreased cFLIP expression, and overexpression of cFLIP contributed to protection from IKK blockade-induced apoptosis in human macrophages. Importantly, IKK blockade also induces RIPK1 kinase-independent apoptosis in human T cells and human pancreatic cells. Altogether, our data indicate that, in contrast to murine cells, blockade of IKK activity in human cells triggers a distinct apoptosis pathway that is independent of RIPK1. These findings have implications for the contribution of RIPK1 to cell death in humans and the efficacy of RIPK1 inhibition in human diseases.
Decrypting crypto: implications of potential financial accounting treatments of cryptocurrency
Authors: Hubbard Benjamin 
Published: June 2023

Purpose
The purpose of this paper is to examine potential financial accounting treatments for cryptocurrencies, including the current guidance, and compare the benefits and shortcomings of each method. It proposes the introduction and use of an intangible asset revaluation model. The study aims to inform both standard setters and financial statement preparers of the most appropriate accounting treatment of this digital asset.

Design/methodology/approach
This paper uses an exploratory analysis and conceptualizes each technical treatment option. For each potential treatment, this paper describes the technical accounting guidance and financial statement implications. The study also uses an illustration to compare the outcomes of each treatment option.

Findings
This paper provides insights into the most appropriate financial accounting treatment of cryptocurrencies. Findings indicate the best option is an intangible asset revaluation model that allows firms to elect a fair value option and record fluctuations in market value to other comprehensive income. This model would improve the accuracy of asset numbers while maintaining the relevance of income amounts by preventing large gains or losses from fair value fluctuations from flowing through the income statement.

Research limitations/implications
The number of firms that hold cryptocurrencies on their balance sheet remains small, thus the research is limited to anecdotal and expository analysis.

Practical implications
The study includes implications for accounting standard setters as they continue to deliberate the appropriate financial accounting treatment for cryptocurrencies. The study can inform the standard setting process and impact future authoritative guidance.

Social implications
The use of cryptocurrency is extremely popular among individual investors and consumers. Updating accounting guidance on crypto can help support a robust crypto market through a useful, informative approach to measurement and reporting. This can also aid in improving the economic prosperity of crypto investors.

Originality/value
This paper fulfills an identified need to examine and understand appropriate accounting guidance for cryptocurrencies. Current guidance has been deemed ineffective and there is debate regarding the proper treatment moving forward. This paper contextualizes this debate and provides suggested solutions.
The impact of Leagile software development on operational performance
Authors: Yadav Ramkaran , Yadav Vinod , Mittal M.L. , Jain Rakesh , Yadav Jigyasa 
Published: June 2023

Purpose
In software development (SD), practitioners have realized the importance of lean thinking. A new term “Leagile” is coined, which is an integrated approach of traditional lean and agile thinking to managing the operations. The study aims to investigate the application of the Leagile principles in a new sector and establish a relationship between the adoption of Leagile approach and operational performance (OP) in SD organizations.

Design/methodology/approach
Empirical research is conducted to investigate the linkage between the Leagile principles and operational measures. Data is collected through surveys from 256 SD industries located in 11 states of India and analysed using the structural equation modelling approach.

Findings
The practitioners envisage a positive impact of adoption of Leagile principles on OP of SD organizations, but one of the principles, i.e. perfection, is unable to influence the performance.

Originality/value
The study contributes by authorizing the contribution of Leagile principles towards OP of SD organizations. The outcomes will motivate the practitioners to enhance the adoption of Leagile principles in SD organizations.
The salience of remote leadership: implications for follower self-control and work-life balance
Authors: Walsh Megan M. , Carleton Erica L. , Ziemer Julie , Ortynsky Mikaila 
Published: June 2023

PurposeThe purpose of this study was to examine whether remote work moderates the mediated relationship between leadership behavior (transformational leadership and leader incivility), followers' self-control, and work-life balance.Design/methodology/approachThe authors conducted a three-wave, time lagged study of 338 followers. Drawing on social information processing theory, a moderated mediation model was proposed: it was hypothesized that remote work strengthens the relationship between leadership behavior (transformational leadership and leader incivility), follower self-control, and subsequent work-life balance (moderated mediation). The theoretical model was tested using OLS regression in SPSS.FindingsThe results show that working remotely strengthens the mediated relationships between leadership behavior, self-control, and work-life balance.Practical implicationsOrganizations need to consider the interaction between remote work and leadership. Leader behaviors have a stronger relationship with follower self-control and work-life balance when the frequency of remote work is higher, so it is important to increase transformational leadership and reduce leader incivility in remote contexts. Leadership training programs and respectful workplace initiatives should be considered.Originality/valueThis study demonstrates the importance of leader behaviors for followers' self-control and work-life balance in relation to remote work. This study is the first to examine the boundary condition of remote work in relation to leadership behavior, follower self-control, and work-life balance.
Leveraging product quality and price for attainment of the manufacturer's economic objectives
Authors: Goswami Mohit , Daultani Yash , Ramkumar M. 
Published: June 2023

PurposeThis paper analytically models and numerically investigates two operating levers, namely optimization of product price and optimization of product quality in the context of a manufacturer that sells the products directly in the marketplace. The study attempts to identify how optimizing product quality and product price can fulfill a manufacturer's economic aims such as maximization of the manufacturer's profit and market demand. Anchored to the extant literature, the demand is modeled as a parametric joint multiplicative function of price and quality. Further, price is modeled as a function of product quality.Design/methodology/approachFirst, the authors evolve the analytical expression for the manufacturer's profit. Thereafter, following the mathematical principles of unconstrained optimization, the authors arrive at the conditions for optimal product quality and product price. The authors further perform numerical experiments to understand the behavior of economic dimensions such as profit and demand with respect to sensitivities associated with cost, quality and price.FindingsThe authors find that under product quality optimization, the optimal product quality is a unique solution in that a highest possible theoretical manufacturer's profit is obtained. However, in the case of product price optimization, the optimal product price is non-unique and is a function of product quality. The authors further find that in the context of functional quality-level expectations, product quality optimization as an operating lever gives a better dividend. However, in the case of higher product quality expectations, product price optimization performs better than product quality optimization. Further, several novel findings are also obtained from numerical experimentations.Originality/valueThe findings of the authors' study have implications for types of industries characterized by relatively low as well as relatively high competitive intensity. Further, as opposed to several extant studies that have often carried out joint optimization of quality and price, the authors' study is one of the first to study the impact of product price and product quality on the manufacturer's economic objective in a disparate and focused manner, thus capturing individual effects.