bluuebunny/crossref_metadata_2025_split huggingface dataset:
HIGH-MOLECULAR-WEIGHT HYALURONAN—A POTENTIAL ADJUVANT TO FLUID RESUSCITATION IN ABDOMINAL SEPSIS?
Authors: Barrueta Tenhunen Annelie , van der Heijden Jaap , Dogné Sophie , Flamion Bruno , Weigl Wojciech , Frithiof Robert , Skorup Paul , Larsson Anders , Larsson Anders , Tenhunen Jyrki 
Published: February 2023

ABSTRACT
While fluid resuscitation is fundamental in the treatment of sepsis-induced tissue hypoperfusion, a sustained positive fluid balance is associated with excess mortality. Hyaluronan, an endogenous glycosaminoglycan with high affinity to water, has not been tested previously as adjuvant to fluid resuscitation in sepsis. In a prospective, parallel-grouped, blinded model of porcine peritonitis sepsis, we randomized animals to intervention with adjuvant hyaluronan (add-on to standard therapy, n = 8) or 0.9% saline (n = 8). After the onset of hemodynamic instability, the animals received an initial bolus of 0.1% hyaluronan (1 mg/kg/10 min) or placebo (0.9% saline) followed by a continuous infusion of 0.1% hyaluronan (1 mg/kg/h) or saline during the experiment. We hypothesized that the administration of hyaluronan would reduce the volume of fluid administered (aiming at stroke volume variation <13%) and/or attenuate the inflammatory reaction. Total volumes of intravenous fluids infused were 17.5 ± 11 versus 19.0 ± 7 mL/kg/h in intervention and control groups, respectively (P = 0.442). Plasma IL-6 increased to 2,450 (1,420–6,890) pg/mL and 3,690 (1,410–11,960) pg/mL (18 hours of resuscitation) in the intervention and control groups (nonsignificant). The intervention counteracted the increase in proportion of fragmented hyaluronan associated with peritonitis sepsis (mean peak elution fraction [18 hours of resuscitation] intervention group: 16.8 ± 0.9 versus control group: 17.9 ± 0.6 [P = 0.031]). In conclusion, hyaluronan did not reduce the volume needed for fluid resuscitation or decrease the inflammatory reaction, even though it counterbalanced the peritonitis-induced shift toward increased proportion of fragmented hyaluronan.
Universal Urogynecologic Consultation and Screening for Fecal Incontinence in Pregnant Women With a History of Obstetric Anal Sphincter Injury: A Cost-Effectiveness Analysis
Authors: Swallow Christina H. , Harvey Christina N. , Harmanli Oz , Shepherd Jonathan P. 
Published: February 2023

Importance
Obstetric anal sphincter injuries (OASIS) predispose for the development of fecal incontinence (FI), but management of subsequent pregnancy after OASIS is controversial.

Objective
We aimed to determine if universal urogynecologic consultation (UUC) for pregnant women with prior OASIS is cost-effective.

Study Design
We performed a cost-effectiveness analysis of pregnant women with a history of OASIS modeling UUC compared with no referral (usual care). We modeled the route of delivery, peripartum complications, and subsequent treatment options for FI. Probabilities and utilities were obtained from published literature. Costs using a third-party payer perspective were gathered from the Medicare physician fee schedule reimbursement data or published literature converted to 2019 U.S. dollars. Cost-effectiveness was determined using incremental cost-effectiveness ratios).

Results
Our model demonstrated that UUC for pregnant patients with prior OASIS was cost-effective. Compared with usual care, the incremental cost-effectiveness ratio for this strategy was $19,858.32 per quality-adjusted life-year, below the willingness to pay a threshold of $50,000/quality-adjusted life-year. Universal urogynecologic consultation reduced the ultimate rate of FI from 25.33% to 22.67% and reduced patients living with untreated FI from 17.36% to 1.49%. Universal urogynecologic consultation increased the use of physical therapy by 14.14%, whereas rates of sacral neuromodulation and sphincteroplasty increased by only 2.48% and 0.58%, respectively. Universal urogynecologic consultation reduced the rate of vaginal delivery from 97.26% to 72.42%, which in turn led to a 1.15% increase in peripartum maternal complications.

Conclusions
Universal urogynecologic consultation in women with a history of OASIS is a cost-effective strategy that decreases the overall incidence of FI, increases treatment utilization for FI, and only marginally increases the risk of maternal morbidity.
Perioperative Outcomes of Colpocleisis at a Single Institution: A Retrospective Cohort Study
Authors: Simms Lauren , Maheshwari Deepali , Harmanli Oz , Rastegar Vida , Diaz Naomi , Jones Keisha 
Published: February 2023

Importance
There is limited literature reporting perioperative outcomes among colpocleisis types.

Objectives
This study aimed to describe perioperative outcomes after colpocleisis at a single institution.

Study Design
Patients who underwent colpocleisis at our academic medical center between August 2009 and January 2019 were included. A retrospective chart review was performed. Descriptive and comparative statistics were generated.

Results
A total of 367 of 409 eligible cases were included. Median follow-up was 44 weeks. There were no major complications or mortalities. Le Fort and posthysterectomy colpocleises were faster than transvaginal hysterectomy (TVH) with colpocleisis (95 and 98 minutes, respectively, vs 123 minutes; P = 0.00) with decreased estimated blood loss (100 and 100 mL, respectively, vs 200 mL; P = 0.000). Urinary tract infection and postoperative incomplete bladder emptying occurred in 22.6% and 13.4% of all patients, respectively, with no difference among the colpocleisis groups (P = 0.83 and P = 0.90). Patients who underwent concomitant sling were not at increased risk of postoperative incomplete bladder emptying (14.7% for Le Fort and 17.2% for total colpocleisis). Prolapse recurred after 0 Le Fort (0%), 6 posthysterectomy (3.7%), and 0 TVH with colpocleisis procedures (0%) (P = 0.02).

Conclusions
Colpocleisis is a safe procedure with a relatively low complication rate. Le Fort, posthysterectomy, and TVH with colpocleisis have similarly favorable safety profiles and very low overall recurrence rates. Concomitant TVH at the time of colpocleisis is associated with increased operative time and increased blood loss. Concomitant sling procedure at the time of colpocleisis does not increase the risk of short-term incomplete bladder emptying.
Hacking Acute Care: A Qualitative Study on the Healthcare Impacts of Ransomware Attacks Against Hospitals
Authors: van Boven Liselotte S. , Kusters Renske W.J. , Tin Derrick , van Osch Frits H. M. , De Cauwer Harald , Ketelings Linsay , Rao Madhura , Dameff Christian , Barten Dennis G. 
Published: February 2023

AbstractBackground and objectivesCyberattacks against healthcare institutions are an increasing threat and have the potential to impair health outcomes. Current research is limited and focuses mainly on the technical consequences, whereas little is known about the healthcare staff experiences and the impact on emergency care, both during the incident and in the recovery phase. This study aims to explore the impact of a sample of large ransomware attacks against hospitals between 2017 and 2022 on acute care delivery and patient care during the recovery phase.MethodsThis interview-based qualitative study assessed the experiences of emergency healthcare professionals and Information and Communication Technology (ICT) staff and investigated the challenges faced when struck by a major hospital ransomware attack. The semi-structured interview guideline was based on current literature and cybersecurity expert consultation. Transcripts were anonymized and information tracing back to participants and/or their organizations was removed for privacy purposes.ResultsNine participants were interviewed, including emergency healthcare providers and ICT-focused staff. Five themes were constructed from the data: impact and challenges regarding patient care continuity, challenges during the recovery process, personal impact on healthcare staff, preparedness, and lessons identified and future recommendations.ConclusionsAccording to the participants of this qualitative study, ransomware attacks have a significant impact on emergency department (ED) workflow, acute patient care and the personal wellbeing of healthcare providers. Preparedness for such incidents is often limited and many challenges are encountered during the acute and recovery phase of the attack. Proactive preparedness efforts are essential to improve contingency planning and to develop response strategies for hospital ransomware attacks.
Chromosome-level genome assembly of tree sparrow reveals a burst of new genes driven by segmental duplications
Authors: Wang Shengnan , Zhang Yingmei , Shen Yue , Lin Zhaocun , Miao Yuquan , Ji Yanzhu , Song Gang 
Published: February 2023

AbstractThe creation of new genes is a major force of evolution. Despite as an important mechanism that generated new genes, segmental duplication (SD) has yet to be accurately identified and fully characterized in birds because the repetitive complexity leads to misassignment and misassembly of sequence. In addition, SD may lead to new gene copies, which makes it possible to test the “out of testis” hypothesis which suggests genes are frequently born with testis-specific expression. Using a high-quality chromosome-level assembly, we performed a systematic analysis and presented a comprehensive landscape of SDs in tree sparrow (Passer montanus). We detected co-localization of newly expanded genes and long terminal repeat retrotransposons (LTR-RTs), both of which are derived from SDs and enriched in microchromosomes. The newly expanded genes are mostly found in eight families includingC2H2ZNF, OR, PIM, PAK, MROH, HYDIN, HSFandITPRIPL. The large majority of new members of these eight families have evolved to pseudogenes, whereas there still some new copies preserved transcriptional activity. Among the transcriptionally active new members, new genes from different families with diverse structures and functions shared a similar testis-biased expression pattern, which is consistent with the “out of testis” hypothesis. Through a case analysis of the high-quality genome assembly of tree sparrow, we reveal that the SDs contribute to the formation of new genes. Our study provides a comprehensive understanding of the emergence, expression and fate of duplicated genes and how the SDs might participate in these processes and shape genome evolution.
Multi-omics and Pharmacological Characterization of Patient-derived Glioma Cell Lines
Authors: Wu Min , Yuan Ran , Ji Nan , Lu Ting , Wang Tingting , Zhang Junxia , Li Mengyuan , Cao Penghui , Zhao Jiarui , Li Guanzhang , Li Jianyu , Li Yu , Tang Yujie , Gao Zhengliang , Wang Xiuxing , Wu Anhua , Cheng Wen , Ge Ming , Cui Gang , You Yongping , Zhang Wei , Wang Qianghu , Chen Jian 
Published: February 2023

AbstractGlioblastoma (GBM) is the most common brain tumor and is currently incurable. Primary GBM cultures are widely used tools for screening potentially therapeutic drugs; however, there is a lack of genomic and pharmacological characterization of these primary GBM cultures. Here, we collected 52 patient-derived glioma cell (PDGC) lines and characterized them through whole- genome sequencing (WGS), RNA-seq, and drug response screening. We identified three molecular subtypes among PDGCs: mesenchymal (MES), proneural (PN), and oxidative phosphorylation (OXPHOS). Upon profiling the responses of PDGCs to 214 drugs, we found that the PN subtype PDGCs were sensitive to tyrosine kinase inhibitors, whereas the OXPHOS subtype PDGCs were sensitive to histone deacetylase inhibitors, oxidative phosphorylation inhibitors, and HMG-CoA reductase inhibitors. PN and OXPHOS subtype PDGCs stably formed tumorsin vivoupon intracranial transplantation into immunodeficient mice, while most MES subtype PDGCs were incapable of tumorigenesisin vivo. In addition, profiling and follow-up investigations showed that the serum-free culture system used for PDGCs enriched and propagated rareMYC/MYCN- amplified glioma cells. Our study provides a resource for understanding primary glioma cell cultures and aiding clinical translation.SignificanceOur study provides a resource for patient-derived glioma cell lines (PDGCs) on transcriptome, genome, drug response, and tumorigenic abilities. PDGCs are categorized into PN, MES, and OXPHOS subtypes, with MES-subtype PDGCs incapable of tumorigenesisin vivo. Notably, the serum-free culture system for PDGCs enriches glioma cells withMYC/MYCNamplification.
Next-generation CRISPR gene-drive systems using Cas12a nuclease
Authors: Juste Sara Sanz , Okamoto Emily M. , Feng Xuechun , Del Amo Victor López 
Published: February 2023

ABSTRACTOne method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a Cas9 nuclease that is constitutively active, impeding our control over their propagation abilities and limiting the generation of novel gene drive arrangements. Yet, other nucleases such as the temperature-sensitive Cas12a have not been explored for gene drive designs. To address this, we herein present a proof-of-concept gene-drive system driven by Cas12a that can be regulated via temperature modulation. Furthermore, we combined Cas9 and Cas12a to build double gene drives capable of simultaneously spreading two independent engineered alleles. The development of Cas12a-mediated gene drives provides an innovative option for designing next-generation vector control strategies to combat disease vectors and agricultural pests.
Experimentation as infrastructure: Enacting transitions differently through diverse economy‐environment assemblages in Aotearoa New Zealand
Authors: Dowell Angus , Lewis Nick , Jones Ryan 
Published: February 2023

AbstractRadically new economic arrangements are needed for just and sustainable transitions to a more environmentally and ecologically resilient world. Yet little progress is being made to imagine the new economy‐environment relations around which resources, actors, and ethics might be configured to enact the novel economic forms needed. This article uses a Social Studies of Economisation and Marketisation (SSEM) approach to examine a suite of differently scaled and structured environmentally focused economic development initiatives in New Zealand. We explore how the initiatives have assembled diverse actors and investment projects into experimental economy‐environment relations. Our account highlights experimentation as a pivotal mode of economisation, and we argue that the initiatives studied by us expose a new experimentation‐led agenda for transitioning to more environmentally and economically just futures. Working with the idea of experimentation in an SSEM framework, we also argue that the diverse initiatives are creating an experimentation infrastructure that provides a more generative platform for novel economy‐environment relations than top–down models of change such as transition pathways. The article opens up a critical politics of environmental economy that focuses attention on emergence, agency, and practice and allows us to reimagine processes of transitioning.
Petroleum Generation Kinetics and Geological Implications for Jurassic Hydrocarbon Source Rocks, Hongqi Depression, Hailar Basin, Northeast China
Authors: XIE Mingxian , MA Feng , CHEN Guangpo , LIU Chang , XIAO Rong , SU Yuping , ZHANG Chengjun 
Published: February 2023

AbstractA sample from the Jurassic Tamulangou Fm. and two comparison samples from the Cretaceous Fm. were used to document the hydrocarbon generation kinetics and phase behaviors at two heating rates using the confined gold tube system. The results show that the different heating rates affect the reaction rates, paths and levels of organic matter evolution. The average activation energy and dominant frequency activation energy of liquid hydrocarbon are significantly lower than those of gaseous. Moreover, igneous intrusion had a positive effect on the blooming, enrichment and preservation of organic matter, promoting a Ro increase of 0.09%–1.07% in the Jurassic Tamulangou Fm. Two models were used to simulate the normal and abnormal evolution caused by thermal events combined hydrocarbon generation kinetic parameters. Thermal simulation analysis shows that oil generation was initially slow and then increased rapidly until a burial depth of 1500 m was reached at ~128 Ma. The largest hydrocarbon expulsion began at ~120 Ma, corresponding to a burial depth of 2450 m. The maximum cumulative yield is 510 mg/g TOC, and it is still in the peak period of hydrocarbon generation, which demonstrates a favorable potential for hydrocarbon exploration.
CCL26 in primary biliary cholangitis – Is it a novel disease mediator?
Authors: Sun Xiaochuan , Cao Yihan , Wang Li , Chen Hua , Zhang Fengchun 
Published: February 2023

AbstractAimTo verify the role of CX3C chemokine ligand 1 ‐ CX3C chemokine receptor 1 (CX3CL1‐CX3CR1) pathway in the pathogenesis of primary biliary cholangitis (PBC). To explore whether CCL26, a novel functional ligand to CX3CR1, participates in the immunological mechanism of PBC.MethodsFifty‐nine PBC patients and 54 healthy controls were recruited. Enzyme‐linked immunosorbent assay and flow cytometry were used to measure CX3CL1 and CCL26 concentrations in plasma and CX3CR1 expression on peripheral lymphocytes, respectively. Chemotactic effects of CX3CL1 and CCL26 toward lymphocytes were detected by Transwell cell migration assays. CX3CL1 and CCL26 expressions in liver were assessed by immunohistochemical staining. Effects of CX3CL1 and CCL26 on stimulating cytokine production from lymphocytes were evaluated using intracellular flow cytometry.ResultsSignificantly elevated CX3CL1 and CCL26 plasma concentration and CX3CR1 expression on CD4+ and CD8+ T cells were noted in PBC patients. CX3CL1 exhibited chemotactic activity toward CD8+ T, natural killer (NK) and NKT cells in a dose‐dependent manner while such chemotactic effects were not detected for CCL26. In PBC patients, CX3CL1 and CCL26 were both increasingly expressed in biliary tracts and a concentration gradient of CCL26 in hepatocytes around portal areas was observed. Immobilized CX3CL1 could enhance interferon‐γ production from T and NK cells while such effect was not exhibited by soluble CX3CL1 or CCL26.ConclusionsCCL26 expression is significantly elevated in plasma and biliary duct of PBC patients, yet does not appear to attract CX3CR1‐expressing immune cells. CX3CL1‐CX3CR1 pathway promotes the infiltration of T, NK and NKT cells into bile ducts and forms a positive feedback loop with T‐helper 1 type cytokines in PBC.
Ambulatory follow‐up among publicly insured children discharged from the emergency department
Authors: Ramgopal Sriram , Rodean Jonathan , Alpern Elizabeth R. , Hall Matt , Chaudhari Pradip P. , Marin Jennifer R. , Shah Samir S. , Freedman Stephen B. , Eltorki Mohamed , Badaki‐Makun Oluwakemi , Shapiro Daniel J. , Rhine Tara , Morse Rustin B. , Neuman Mark I. 
Published: February 2023

AbstractBackgroundWhile children discharged from the emergency department (ED) are frequently advised to follow up with ambulatory care providers, the extent to which this occurs is unknown. We sought to characterize the proportion of publicly insured children who have an ambulatory visit following ED discharge, identify factors associated with ambulatory follow‐up, and evaluate the association of ambulatory follow‐up with subsequent hospital‐based health care utilization.MethodsWe performed a cross‐sectional study of pediatric (<18 years) encounters during 2019 included in the IBM Watson Medicaid MarketScan claims database from seven U.S. states. Our primary outcome was an ambulatory follow‐up visit within 7 days of ED discharge. Secondary outcomes were 7‐day ED return visits and hospitalizations. Logistic regression and Cox proportional hazards were used for multivariable modeling.ResultsWe included 1,408,406 index ED encounters (median age 5 years, IQR 2–10 years), for which a 7‐day ambulatory visit occurred in 280,602 (19.9%). Conditions with the highest proportion of 7‐day ambulatory follow‐up included seizures (36.4%); allergic, immunologic, and rheumatologic diseases (24.6%); other gastrointestinal diseases (24.5%); and fever (24.1%). Ambulatory follow‐up was associated with younger age, Hispanic ethnicity, weekend ED discharge, ambulatory encounters prior to the ED visit, and diagnostic testing performed during the ED encounter. Ambulatory follow‐up was inversely associated with Black race and ambulatory care–sensitive or complex chronic conditions. In Cox models, ambulatory follow‐up was associated with a higher hazard ratio (HR) of subsequent ED return (HR range 1.32–1.65) visit and hospitalization (HR range 3.10–4.03).ConclusionsOne‐fifth of children discharged from the ED have an ambulatory visit within 7 days, which varied by patient characteristics and diagnoses. Children with ambulatory follow‐up have a greater subsequent health care utilization, including subsequent ED visit and/or hospitalization. These findings identify the need to further research the role and costs associated with routine post‐ED visit follow‐up.
Early postnatal weight loss does not affect the 2‐year neurodevelopment in preterm infants of less than 32 weeks
Authors: Correani Alessio , Biagetti Chiara , Burattini Ilaria , Bellagamba Maria Paola , D'Ascenzo Rita , Ferretti Enrica , Peretti Anna , Monachesi Chiara , Simonato Manuela , Carnielli Virgilio 
Published: February 2023

AbstractAimIt is still unclear if the magnitude of early postnatal weight loss (PWL) could be associated with neurodevelopmental outcomes in preterm infants. We studied the association between PWL and neurodevelopment at 2‐year corrected age in preterm infants.MethodsWe retrospectively reviewed data of preterm infants with a gestational age between 24 + 0 and 31 + 6 weeks/days, admitted at the G.Salesi Children's Hospital, Ancona, Italy, between 1 January 2006 and 31 December 2019. Infants with PWL greater than or equal to 10% (PWL ≥ 10%) were compared with those with PWL of less than 10% (PWL < 10%). A matched cohort analysis was also performed using gestational age and birth weight as matching variables.ResultsWe analysed 812 infants: 471 (58%) PWL ≥ 10% and 341 (42%) PWL < 10%. A subgroup of 247 PWL ≥ 10% was closely match‐paired with 247 PWL < 10% infants. There were no differences in amino acid and energy intakes from birth to day 14 of life and from birth to 36 weeks. Although at 36 weeks, body weight and total length were lower in PWL ≥ 10% than PWL < 10%, anthropometry and neurodevelopment at 2 years were similar between groups.ConclusionGiven similar amino acid and energy intakes on PWL ≥ 10% and PWL < 10% preterm infants of less than 32 + 0 weeks/days, PWL does not affect 2‐year neurodevelopment.
The development of sleep‐like posture expression with age in female Holstein calves
Authors: Fukasawa Michiru 
Published: February 2023

AbstractSleep plays an important role in cattle production and welfare. Therefore, this study aimed to investigate the development of sleep‐like posture (SLP) expression in dairy calves from birth to the first calving as an indicator of their sleep behavior. Fifteen female Holstein calves were subjected. Daily SLP was measured eight times (0.5, 1, 2, 4, 8, 12, and 18 months and 23 months or 1 month before the first calving) using an accelerometer. Calves were kept in individual pens until weaning at 2.5 months old and then kept in the group. The daily SLP time decreased rapidly in early life; however, the rate of decrease gradually slowed and eventually reached a stable level of approximately 60 min/day after 12 months old. The daily SLP bout frequency showed the same change as the SLP time. In contrast, the averaged SLP bout duration slowly decreased with age. Longer daily SLP time during early life in female Holstein calves might be related to brain development. The individual expression of daily SLP time differs before and after weaning. Some external and/or internal factors associated with weaning may be involved SLP expression.