bluuebunny/crossref_metadata_2025_split huggingface dataset:
Larval and adult experience and ecotype affect oviposition behavior in a niche-expanding butterfly
Authors: Braem Simon , Van Dyck Hans 
Published: May 2023

AbstractLocal adaptation, early-developmental and behavioral plasticity have all been suggested to be underlying mechanisms of behavioral variation. They allow organisms to cope with resource heterogeneity in time and space. However, the relative contribution of each of these drivers on niche-expansion success is still unclear. We addressed this issue by studying oviposition behavior in a butterfly (Pararge aegeria) that used to be confined to forest, but recently colonized anthropogenic areas too. We caught females of either forest or agricultural ecotype and allowed their larval offspring to experience early-habitat cues in either open field or woodland conditions. For 110 females of this transplant experiment, we monitored all oviposition-related behaviors (i.e., site selection, searching, motivation, learning) using repeated trials in outdoor flight cages with naturally occurring host grasses. We observed changes in oviposition site preference, with increasing preference for semi-shaded host grass (by vegetation or vertical structures) over consecutive egg-lays. Motivation to oviposit was most affected by larval experience (in interaction with cage environment). We also show evidence for ecotypic differentiation with the agricultural ecotype showing reduced search effort during the first trial compared to the forest ecotype. We argue that females of agricultural landscapes with fragmented resource distributions adopt a more risk-aversive oviposition strategy. Our detailed behavioral tracking under ecologically relevant conditions, stresses the importance of considering different types of behavioral plasticity to unravel a species’ success during niche expansion.
A New Stability-Indicating RP-HPLC Method for Simultaneous Quantification of Diacerein and Aceclofenac in Novel Nanoemulgel Formulation and Commercial Tablet Dosage Form in the Presence of their Major Degradation Products Using DoE Approach
Authors: Kazi Taufik M , Dandagi Panchaxari M 
Published: May 2023

AbstractThe present study aimed to develop a simple, robust, sensitive and effective stability-indicating reversed-phase high-performance liquid chromatography method for simultaneous quantification of diacerein (DCN) and aceclofenac (ACE) in novel nanoemulgel formulation and commercial tablets in the presence of their main degradation product: rhein (RH) and diclofenac sodium (DLS), respectively. A fractional factorial design was used to screen the crucial independent factors, whereas a central composite design was used for the optimization of the chromatographic conditions. The separation was carried out on Phenomenex C18 column (5 μm, 250 × 4.6 mm), using a mobile phase consisting of phosphate buffer pH 3 (0.1% v/v orthophosphoric acid) and acetonitrile (40:60 v/v) at a flow rate of 1 mL/min with detection at 264 nm. The analytes were exposed to a variety of stress conditions, including heat, alkali, acid, oxidation, photochemical, humidity and hydrolysis. DCN, ACE, RH and DLS were found to have retention times of 4.32 ± 0.15, 5.77 ± 0.07, 8.28 ± 0.20 and 9.10 ± 0.18 min, respectively. The percent recovery for all four analytes was found to be between 98 and 102, and the procedure was discovered to be linear in the range of 0.1–64 μg/mL with R2 value &gt; 0.999. The established method was validated as per ICH guidelines and successfully used to assay DCN and ACE in their combined marketed tablet dosage form and developed nanoemulgel formulation.
Pyoderma Gangrenosum Is Associated With Increased Risk of Inflammatory Pouch-Related Complications: A Retrospective Cohort Study
Authors: Panganiban Ronaldo Paolo , Tuan Alyssa , Hart Maxwell , Pelton Mathew , Mikhail Daniella , Akhtar Sarah , Bogale Kaleb , Deiling Susan , Zhou Shouhao , Coates Mathew D , Yochum Gregory S , Koltun Walter 
Published: May 2023

Abstract

Background
Pyoderma gangrenosum (PG) is a rare, neutrophilic dermatosis that is a well-established extraintestinal manifestation (EIM) of inflammatory bowel disease. The clinical implications of developing PG in patients with ulcerative colitis (UC) who undergo total proctocolectomy colectomy and ileal pouch anal anastomosis (TPC-IPAA) surgery remain unknown.

Methods
Study participants were selected from patients enrolled in the Carlino Family Inflammatory Bowel and Colorectal Disease Biobank between 1998 and 2021 with a pre-colectomy diagnosis of UC and who underwent TPC-IPAA surgery. A retrospective study comparing patients with PG and those without PG was performed. The outcomes measured included the development of pouchitis, pouchitis classification, presence of pouch fistula, anal fistula, anal stenosis, and pouch failure.

Results
In this study, 357 IPAA patients were included, 10 of whom suffered PG. Patients with PG and without PG had similar demographics and clinical characteristics. Both groups had similar rates of pouchitis (80% in PG patients and 64% in patients without PG, P = .504). However, IPAA patients with PG had a higher risk of developing pouch fistula (50% vs 10%, P = .002), anal fistula (40% vs 12%, P = .031), and Crohn’s-like disease of the pouch (70% vs 15%, P = .003) compared to patients without PG. Patients who developed PG prior to their first episode of pouchitis were more likely to eventually experience pouch failure (odds ratio: 20.7, 95% confidence interval: 3.9, 110.7, q = 0.003 after false discovery rate adjustment).

Conclusions
Among UC patients who undergo TPC-IPAA surgery, the development of PG portends poor pouch outcomes and is predictive of pouch failure.
Clinical relevance and prognostic value of renal Doppler in acute decompensated precapillary pulmonary hypertension
Authors: Pichon Jérémie , Roche Anne , Fauvel Charles , Boucly Athénais , Mercier Olaf , Ebstein Nathan , Beurnier Antoine , Cortese Jonathan , Jevnikar Mitja , Jaïs Xavier , Fartoukh Muriel , Fadel Elie , Sitbon Olivier , Montani David , Voiriot Guillaume , Humbert Marc , Savale Laurent 
Published: May 2023

Abstract

Aims
We aim to evaluate the clinical relevance and the prognostic value of arterial and venous renal Doppler in acute decompensated precapillary pulmonary hypertension (PH).

Methods and results
The renal resistance index (RRI) and the Doppler-derived renal venous stasis index (RVSI) were monitored at admission and on Day 3 in a prospective cohort of precapillary PH patients managed in intensive care unit for acute right heart failure (RHF). The primary composite endpoint included death, circulatory assistance, urgent transplantation, or rehospitalization for acute RHF within 90 days following inclusion. Ninety-one patients were enrolled (58% female, age 58 ± 16 years). The primary endpoint event occurred in 32 patients (33%). In univariate logistic regression analysis, variables associated with RRI higher than the median value were non-variable parameters (age and history of hypertension), congestion (right atrial pressure and renal pulse pressure), cardiac function [tricuspid annular plane systolic excursion (TAPSE) and left ventricular outflow tract- velocity time integral], systemic pressures and NT-proBNP. Variables associated with RVSI higher than the median value were congestion (high central venous pressure, right atrial pressure, and renal pulse pressure), right cardiac function (TAPSE), severe tricuspid regurgitation, and systemic pressures. Inotropic support was more frequently required in patients with high RRI (P = 0.01) or high RVSI (P = 0.003) at the time of admission. At Day 3, a RRI value &lt;0.9 was associated with a better prognosis after adjusting to the estimated glomerular filtration rate.

Conclusion
Renal Doppler provides additional information to assess the severity of patients admitted to the intensive care unit for acute decompensated precapillary PH.
Causal relationships between the gut microbiome, blood lipids, and heart failure: a Mendelian randomization analysis
Authors: Dai Huajie , Hou Tianzhichao , Wang Qi , Hou Yanan , Wang Tiange , Zheng Jie , Lin Hong , Zhao Zhiyun , Li Mian , Wang Shuangyuan , Zhang Di , Dai Meng , Zheng Ruizhi , Lu Jieli , Xu Yu , Chen Yuhong , Ning Guang , Wang Weiqing , Bi Yufang , Xu Min 
Published: May 2023

Abstract

Aims
Studies have linked gut microbiome and heart failure (HF). However, their causal relationships and potential mediating factors have not been well defined. To investigate the causal relationships between the gut microbiome and HF and the mediating effect of potential blood lipids by using genetics.

Methods and results
We performed a bidirectional and mediation Mendelian randomization (MR) study using summary statistics from the genome-wide association studies of gut microbial taxa (Dutch Microbiome Project, n = 7738), blood lipids (UK Biobank, n = 115 078), and a meta-analysis of HF (115 150 cases and 1550 331 controls). We applied the inverse–variance weighted estimation method as the primary method, with several other estimators as complementary methods. The multivariable MR approach based on Bayesian model averaging (MR-BMA) was used to prioritize the most likely causal lipids. Six microbial taxa are suggestively associated with HF causally. The most significant taxon was the species Bacteroides dorei [odds ratio = 1.059, 95% confidence interval (CI) = 1.022–1.097, P-value = 0.0017]. The MR-BMA analysis showed that apolipoprotein B (ApoB) was the most likely causal lipid for HF (the marginal inclusion probability = 0.717, P-value = 0.005). The mediation MR analysis showed that ApoB mediated the causal effects of species B. dorei on HF (proportion mediated = 10.1%, 95% CI = 0.2–21.6%, P-value = 0.031).

Conclusion
The study suggested a causal relationship between specific gut microbial taxa and HF and that ApoB might mediate this relationship as the primary lipid determinant of HF.
The vulnerability to COVID-19 of migrants in large urban areas: structural exacerbators and community-level mitigators
Authors: Hitch Lisa , Masoud Dima , Hobbs Laura Ansley , Moujabber Marvy , Cravero Kathleen 
Published: May 2023

Abstract

Background
Despite research on large urban areas in the context of COVID-19, evidence on how these settings impact migrants is still limited.

Objective
To explore exacerbating and mitigating factors of large urban areas on migrants’ vulnerabilities during the COVID-19 pandemic.

Methods
We conducted a systematic review of peer-reviewed studies published between 2020 and 2022, focused on migrants (foreign-born individuals who have not been naturalized in the host country, regardless of legal immigration status) in urban areas with a population &gt;500 000. After screening 880 studies, 29 studies were included and categorized within the following thematic framework: (i) pre-existing inequities, (ii) governance strategies, (iii) urban design and (iv) engagement of civil society organizations (CSOs).

Results
Exacerbating factors include pre-existing inequities (e.g. unemployment, financial instability and barriers to healthcare access), exclusionary government responses (e.g. ineligibility for relief funds or unemployment benefits) and residential segregation. Mitigating community-level factors include the engagement of CSOs to fill institutional and governmental gaps through service provision and use of technology.

Conclusions
We recommend increased attention to pre-existing structural inequities faced by migrants, more inclusive governance strategies and partnerships between government and CSOs to improve the design and delivery of services to migrants in large urban areas. More research is needed on how urban design can be utilized to mitigate COVID-19 impacts on migrant communities. The factors identified in this systematic review should be considered as part of migrant-inclusive emergency preparedness strategies to address the disproportionate impact of health crises on migrant communities.
The best laid plans?: international governance perspectives in AMR national action plans in Europe
Authors: Carelli Daniel E , Mitsouli Elisavet T , Ogne Josefin B , Pierre Jon 
Published: May 2023

Abstract

Background
There is a broad consensus in the European public health community that international collaboration and coordination are key in the fight against antimicrobial resistance (AMR). Yet, while experts typically testify the importance of cross-national learning and a coordinated effort to reduce the spread of multi-resistant bacteria, there are mixed views about how that is best realized in practice, particularly on the dichotomy between horizontal and vertical activities.

Methods
National action plans (NAP) from all EU member states were systematically overviewed by two independent researchers. We followed a fixed procedure for locating a broad and comparable content on the international dimensions, and letting these vary on scales.

Results
We find that countries adhere to four different strategies for international coordination, varying between ‘high’ and ‘low’ on values of vertical and horizontal activities. Most countries spend none or very little space to discuss international activities, while some countries use their NAPs to outline their aspiration for taking leading roles in the international struggle. Moreover, in line with previous research, we find that many countries directly mimic the Global Action Plan, but also that a large share of countries describe independent arrangements in their international strategies.

Conclusions
European countries recognize AMR and its inherent international governance challenge differently in their NAPs, which may have implications for coordinated action to address the issue.
The small intestine: dining table of host–microbiota meetings
Authors: Delbaere Karen , Roegiers Inez , Bron Auriane , Durif Claude , Van de Wiele Tom , Blanquet-Diot Stéphanie , Marinelli Ludovica 
Published: May 2023

AbstractGrowing evidence suggests the importance of the small intestinal bacteria in the diet–host–microbiota dialogue in various facets of health and disease. Yet, this body site is still poorly explored and its ecology and mechanisms of interaction with the host are just starting to be unraveled. In this review, we describe the current knowledge on the small intestinal ecology, its composition and diversity, and how the intestinal bacteria in homeostatic conditions participate in nutrient digestion and absorption. We illustrate the importance of a controlled bacterial density and of the preservation of absorptive surface for the host’s nutritional status. In particular, we discuss these aspects of the small intestinal environment in the framework of two disease conditions, namely small intestinal bacterial overgrowth (SIBO) and short bowel syndrome (SBS). We also detail in vivo, ex vivo, and in vitro models developed to simulate the small intestinal environment, some applied for (diet–)host–bacteria interaction studies. Lastly, we highlight recent technological, medical, and scientific advances applicable to investigate this complex and yet understudied body environment to broaden our knowledge in support of further progress in the medical practice, and to proceed towards the integration of the (small)intestinal bacteria in personalized therapeutic approaches.
The Molecular Circadian Clock of Phox2b-expressing Cells Drives Daily Variation of the Hypoxic but Not Hypercapnic Ventilatory Response in Mice
Authors: Jones Aaron A , Marino Gabriella M , Spears Allison R , Arble Deanna M 
Published: May 2023

Abstract
While the suprachiasmatic nucleus (SCN) controls 24-h rhythms in breathing, including minute ventilation (VE), the mechanisms by which the SCN drives these daily changes are not well understood. Moreover, the extent to which the circadian clock regulates hypercapnic and hypoxic ventilatory chemoreflexes is unknown. We hypothesized that the SCN regulates daily breathing and chemoreflex rhythms by synchronizing the molecular circadian clock of cells. We used whole-body plethysmography to assess ventilatory function in transgenic BMAL1 knockout (KO) mice to determine the role of the molecular clock in regulating daily rhythms in ventilation and chemoreflex. Unlike their wild-type littermates, BMAL1 KO mice exhibited a blunted daily rhythm in VE and failed to demonstrate daily variation in the hypoxic ventilatory response (HVR) or hypercapnic ventilatory response (HCVR). To determine if the observed phenotype was mediated by the molecular clock of key respiratory cells, we then assessed ventilatory rhythms in BMAL1fl/fl; Phox2bCre/+ mice, which lack BMAL1 in all Phox2b-expressing chemoreceptor cells (hereafter called BKOP). BKOP mice lacked daily variation in HVR, similar to BMAL1 KO mice. However, unlike BMAL1 KO mice, BKOP mice exhibited circadian variations in VE and HCVR comparable to controls. These data indicate that the SCN regulates daily rhythms in VE, HVR, and HCVR, in part, through the synchronization of the molecular clock. Moreover, the molecular clock of Phox2b-expressing cells is specifically necessary for daily variation in the hypoxic chemoreflex. These findings suggest that disruption of circadian biology may undermine respiratory homeostasis, which, in turn, may have clinical implications for respiratory disease.
Probabilistic forecasting of daily COVID-19 admissions using machine learning
Authors: Rostami-Tabar Bahman , Arora Siddharth , Rendon-Sanchez Juan F , Goltsos Thanos E 
Published: May 2023

Abstract
Accepted by: Aris Syntetos
Accurate forecasts of daily Coronavirus-2019 (COVID-19) admissions are critical for healthcare planners and decision-makers to better manage scarce resources during and around infection peaks. Numerous studies have focused on forecasting COVID-19 admissions at the national or global levels. Localized predictions are vital, as they allow for resource planning redistribution, but also scarce and harder to get right. Several possible indicators can be used to predict COVID-19 admissions. The inherent variability in the admissions necessitates the generation and evaluation of the forecast distribution of admissions, as opposed to producing only a point forecast. In this study, we propose a quantile regression forest (QRF) model for probabilistic forecasting of daily COVID-19 admissions for a local hospital trust (aggregation of 3 hospitals), up to 7 days ahead, using a multitude of different predictors. We evaluate point forecast accuracy as well as the accuracy of the forecast distribution using appropriate measures. We provide evidence that QRF outperforms univariate time series methods and other more sophisticated benchmarks. Our findings also show that lagged admissions, total positive cases, daily tests performed, and Google grocery and Apple driving are the most salient predictors. Finally, we highlight areas where further research is needed.
Large Deviation Principle for Random Permutations
Authors: Borga Jacopo , Das Sayan , Mukherjee Sumit , Winkler Peter 
Published: May 2023

Abstract
We derive a large deviation principle for random permutations induced by probability measures of the unit square, called permutons. These permutations are called $\mu $-random permutations. We also introduce and study a new general class of models of random permutations, called Gibbs permutation models, which combines and generalizes $\mu $-random permutations and the celebrated Mallows model for permutations. Most of our results hold in the general setting of Gibbs permutation models. We apply the tools that we develop to the case of $\mu $-random permutations conditioned to have an atypical proportion of patterns. Several results are made more concrete in the specific case of inversions. For instance, we prove the existence of at least one phase transition for a generalized version of the Mallows model where the base measure is non-uniform. This is in contrast with the results of Starr (2009, 2018) on the (standard) Mallows model, where the absence of phase transition, that is, phase uniqueness, was proven. Our results naturally lead us to investigate a new notion of permutons, called conditionally constant permutons, which generalizes both pattern-avoiding and pattern-packing permutons. We describe some properties of conditionally constant permutons with respect to inversions. The study of conditionally constant permutons for general patterns seems to be a new challenging problem.
Acute inflammation alters lung lymphocytes and potentiates innate-like behavior in young mouse lung CD8 T cells, resembling lung CD8 T cells from old mice
Authors: Piergallini Tucker J , Scordo Julia M , Allué-Guardia Anna , Pino Paula A , Zhang Hao , Cai Hong , Wang Yufeng , Schlesinger Larry S , Torrelles Jordi B , Turner Joanne 
Published: May 2023

AbstractInflammation plays a significant role in lung infection including that caused by Mycobacterium tuberculosis, in which both adaptive and innate lymphocytes can affect infection control. How inflammation affects infection is understood in a broad sense, including inflammaging (chronic inflammation) seen in the elderly, but the explicit role that inflammation can play in regulation of lymphocyte function is not known. To fill this knowledge gap, we used an acute lipopolysaccharide (LPS) treatment in young mice and studied lymphocyte responses, focusing on CD8 T cell subsets. LPS treatment decreased the total numbers of T cells in the lungs of LPS mice while also increasing the number of activated T cells. We demonstrate that lung CD8 T cells from LPS mice became capable of an antigen independent innate-like IFN-γ secretion, dependent on IL-12p70 stimulation, paralleling innate-like IFN-γ secretion of lung CD8 T cells from old mice. Overall, this study provides information on how acute inflammation can affect lymphocytes, particularly CD8 T cells, which could potentially affect immune control of various disease states.
The role of m6A mRNA modification in normal and malignant hematopoiesis
Authors: Ma Zhangjing , Sugimura Rio , Lui Kathy O 
Published: May 2023

Abstract
Hematopoiesis is a highly orchestrated biological process sustaining the supply of leukocytes involved in the maintenance of immunity, O2 and CO2 exchange, and wound healing throughout the lifetime of an animal, including humans. During early hematopoietic cell development, several waves of hematopoiesis require the precise regulation of hematopoietic ontogeny as well as the maintenance of hematopoietic stem and progenitor cells in the hematopoietic tissues, such as the fetal liver and bone marrow. Recently, emerging evidence has suggested the critical role of m6A messenger RNA (mRNA) modification, an epigenetic modification dynamically regulated by its effector proteins, in the generation and maintenance of hematopoietic cells during embryogenesis. In the adulthood, m6A has also been demonstrated to be involved in the functional maintenance of hematopoietic stem and progenitor cells in the bone marrow and umbilical cord blood, as well as the progression of malignant hematopoiesis. In this review, we focus on recent progress in identifying the biological functions of m6A mRNA modification, its regulators, and downstream gene targets during normal and pathological hematopoiesis. We propose that targeting m6A mRNA modification could offer novel insights into therapeutic development against abnormal and malignant hematopoietic cell development in the future.