bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
Authors: Xu Zhe , Gong Boyang , Li Zhaodong , Wang Ying , Zhao Zeyu , Xie Lulu , Peng Yanfei , Zhao Shuwu , Zhou Huifang , Bian Yuhong 
Published: August 2023

AbstractBazi Bushen, a Chinese‐patented drug with the function of relieving fatigue and delaying ageing, has been proven effective for extenuating skin senescence. To investigate the potential mechanism, senescence‐accelerated mouse prone 6 (SAMP6) was intragastrically administered with Bazi Bushen for 9 weeks to induce skin homeostasis. Skin homeostasis is important in mitigating skin senescence, and it is related to many factors such as oxidative stress, SASP, apoptosis, autophagy and stem cell. In our study, skin damage in SAMP6 mice was observed using HE, Masson and SA‐β‐gal staining. The content of hydroxyproline and the activities of SOD, MDA, GSH‐PX and T‐AOC in the skin were measured using commercial assay kits. The level of SASP factors (IL‐6, IL‐1β, TNF‐α, MMP2 and MMP9) in skin were measured using ELISA kits. The protein expressions of p16, p21, p53, Bax, Bcl‐2, Cleaved caspase‐3, LC3, p62, Beclin1, OCT4, SOX2 and NANOG were measured by western blotting. The expression of ITGA6 and COL17A1 was measured by immunofluorescence staining and western blotting. Our findings demonstrated that Bazi Bushen alleviated skin senescence by orchestrating skin homeostasis, reducing the level of oxidative stress and the expression of SASP, regulating the balance of apoptosis and autophagy and enhancing the protein expressions of ITGA6 and COL17A1 to improve skin structure in SAMP6 mice. This study indicated that Bazi Bushen could serve as a potential therapy for alleviating skin senescence.
27‐Hydroxycholesterol represses G9a expression via oestrogen receptor alpha in breast cancer
Authors: Vini Ravindran , Lekshmi Asha , Ravindran Swathy , Thulaseedharan Jissa Vinoda , Sujathan Kunjuraman , Rajavelu Arumugam , Sreeja Sreeharshan 
Published: August 2023

Abstract27‐hydroxycholesterol (27‐HC) is a cholesterol metabolite and the first discovered endogenous selective estrogen receptor modulator (SERM) that has been shown to have proliferative and metastatic activity in breast cancer. However, whether 27‐HC metabolite modulates the epigenetic signatures in breast cancer and its progression remains unclear. The current study, reports that 27‐HC represses the expression of euchromatic histone lysine methyltransferase G9a, further reducing di‐methylation at H3K9 in a subset of genes. We also observed reduced occupancy of ERα at the G9a promoter, indicating that 27‐HC negatively regulates the ERα occupancy on the G9a promoter and functions as a transcriptional repressor. Further, ChIP‐sequencing for the H3K9me2 mark has demonstrated that 27‐HC treatment reduces the H3K9me2 mark on subset of genes linked to cancer progression, proliferation, and metastasis. We observed upregulation of these genes following 27‐HC treatment which further confirms the loss of methylation at these genes. Immunohistochemical analysis with breast cancer patient tissues indicated a positive correlation between G9a expression and CYP7B1, a key enzyme of 27‐HC catabolism. Overall, this study reports that 27‐HC represses G9a expression via ERα and reduces the levels of H3K9me2 on a subset of genes, including the genes that aid in breast tumorigenesis and invasion further, increasing its expression in the breast cancer cells.
White paper on psychodermatology in Europe: A position paper from the EADV Psychodermatology Task Force and the European Society for Dermatology and Psychiatry (ESDaP)
Authors: Misery Laurent , Schut Christina , Balieva Flora , Bobko Svetlana , Reich Adam , Sampogna Francesca , Altunay Ilknur , Dalgard Florence , Gieler Uwe , Kupfer Jörg , Lvov Andrey , Poot Françoise , Szepietowski Jacek C. , Tomas‐Aragones Lucia , Vulink Nienke , Zalewska‐Janowska Anna , Bewley Anthony 
Published: August 2023

AbstractPsychodermatology is a subspecialty of dermatology that is of increasing interest to dermatologists and patients. The case for the provision of at least regional psychodermatology services across Europe is robust. Psychodermatology services have been shown to have better, quicker and more cost‐efficient clinical outcomes for patients with psychodermatological conditions. Despite this, psychodermatology services are not uniformly available across Europe. In fact many countries have yet to establish dedicated psychodermatology services. In other countries psychodermatology services are in development. Even in countries where psychodermatolgy units have been established, the services are not available across the whole country. This is especially true for the provision of paediatric psychodermatology services. Also whilst most states across Europe are keen to develop psychodermatology services, the rate at which this development is being implemented is very slow. Our paper maps the current provision of psychodermatology services across Europe and indicates that there is still very much more work to be done in order to develop the comprehensive psychodermatology services across Europe, which are so crucial for our patients.
Long‐term effect of letrozole on metastatic uterine smooth muscle tumors of uncertain malignant potential: A case report
Authors: Praben Padiachy , Kido Hidenori , Terao Yasuhisa , Takagi Tatsuya , Hayashi Takuo , Naito Toshio , Kato Shunsuke 
Published: August 2023

AbstractA 48‐year‐old woman underwent total abdominal hysterectomy and right salpingo‐oophorectomy and was initially diagnosed with a uterine leiomyoma and right ovarian cystadenoma. After 4 years, multiple pulmonary metastases were identified, and treatment with gonadotropin‐releasing hormone agonists was started, but stopped later due to disease progression. The patient developed dyspnea and underwent right upper lobectomy. The histopathological findings were consistent with those of pulmonary metastases secondary to a uterine smooth muscle tumor of uncertain malignant potential. Slow disease progression after a poor response to adriamycin and hormone receptor positivity led to the start of letrozole. Letrozole induced spontaneous regression of the pulmonary metastases, and about 2 years into the treatment, sustained response was achieved with minimal side effects. This may be the first case supporting the long‐term efficacy and safety of letrozole in the management of adriamycin‐resistant lung metastases of uterine smooth muscle tumors of uncertain malignant potential.
Toward a non‐binary semiotics of intersectionality: linguistic anthropology in the wake of coloniality
Authors: Ke‐Schutte Jay , Babcock Joshua 
Published: August 2023

AbstractThis special issue proposes a non‐binary semiotics of intersectionality to both draw attention to and unsettle binary participation frameworks of “the‐West‐and‐its‐others.” Contributors demonstrate how intersectionality can reconfigure scholarly approaches to the semiotic analysis of social life, expanding the bounds of the ethnographic as both genre and site of ideological work while also suggesting new stakes for conceptualizations of the personal beyond static, neoliberal presuppositions of the identity‐bearing individual. This proposed reorientation has stakes for the study of race–language co‐naturalizations in locations reflexively cast as beyond white settler‐colonial contexts. We place the study of intersectionality within the historical socius of the colonial and its prefixes (de‐, post‐, and anti‐) by engaging with the historical and material conditions of human capital and land enclosure out of which Kimberlé Crenshaw’s micro‐interactional observations emerged as originary reflections on the concept of intersectionality. Together, we consider linguistic and co(n)textual phenomena that are left out of most contemporary intersectional and critical race analyses. The authors demonstrate an array of modalities through which we can analytically separate intersectionality‐as‐method, while not assuming American monolingual racial experiences as universal.
Common genetic and environmental bases of the mental disorders and personality traits: Special focus on the hierarchical model of psychopathology and NEO‐PI‐R facets
Authors: Mitrović Dušanka , Mihić Ljiljana , Sadiković Selka , Smederevac Snežana 
Published: August 2023

AbstractObjectiveThis study examined whether phenotypic correlations between psychopathological dimensions and personality traits of different hierarchical levels originate from common genetic and environmental sources of variance.MethodParticipants were 386 monozygotic and 204 dizygotic twins. The Psychiatric Diagnostic Screening Questionnaire (PDSQ) was applied along with the Revised NEO Personality Inventory (NEO‐PI‐R). The results of the CFA confirmed the hypothesis of the internalizing and externalizing dimensions underlying PDSQ scales.ResultsThe results indicated a significantly greater role of genetic compared to environmental factors in the relationship between internalizing psychopathology and personality traits. Facets of neuroticism showed positive genetic links with internalizing disorders, while negative genetic links were shown for all facets of extraversion except excitement‐seeking, competence, self‐discipline, achievement striving, actions, and trust. Lower‐order personality traits were shown to be associated with internalizing disorders more intensively than the broader domains to which they belong, both at the phenotypic and genetic levels.ConclusionsHigh neuroticism, together with several facets from the domain of extraversion and conscientiousness seems to represent an increased genetic susceptibility to the disorders from the internalizing spectrum. Results also suggest that specific environmental factors which are not shared with personality traits contribute to the internalizing symptoms.
Subcellular proteomics for determining iron‐limited remodeling of plastids in the model diatom Thalassiosira pseudonana (Bacillariophyta)
Authors: Gomes Kristofer M. , Nunn Brook L. , Chappell P. Dreux , Jenkins Bethany D. 
Published: August 2023

AbstractDiatoms are important primary producers in the world's oceans, yet their growth is constrained in large regions by low bioavailable iron (Fe). Low‐Fe stress‐induced limitation of primary production is due to requirements for Fe in components of essential metabolic pathways including photosynthesis and other chloroplast plastid functions. Studies have shown that under low‐Fe stress, diatoms alter plastid‐specific processes, including components of electron transport. These physiological changes suggest changes of protein content and in protein abundances within the diatom plastid. While in silico predictions provide putative information on plastid‐localized proteins, knowledge of diatom plastid proteins remains limited in comparison to well‐studied model photosynthetic organisms. To address this, we employed shotgun proteomics to investigate the proteome of subcellular plastid‐enriched fractions from Thalassiosira pseudonana to gain a better understanding of how the plastid proteome is remodeled in response to Fe limitation. Using mass spectrometry‐based peptide identification and quantification, we analyzed T. pseudonana grown under Fe‐replete and ‐limiting conditions. Through these analyses, we inferred the relative quantities of each protein, revealing that Fe limitation regulates major metabolic pathways in the plastid, including the Calvin cycle. Additionally, we observed changes in the expression of light‐harvesting proteins. In silico localization predictions of proteins identified in this plastid‐enriched proteome allowed for an in‐depth comparison of theoretical versus observed plastid‐localization, providing evidence for the potential of additional protein import pathways into the diatom plastid.
Religious Identity‐Inconsistent Attending: Its Correlates and Political Implications
Authors: Djupe Paul A. , Burge Ryan P. , Garneau Christopher R.H. 
Published: August 2023

AbstractThe foundation of religious measurement in surveys presumes that individual religious affiliation (“What is your present religion, if any?”) accurately describes the religious community in which respondents are involved. But what if it doesn't? In a recent survey of 4,000 Americans, we asked whether their current congregation matches their religious identity and about a fifth of Americans indicated that it does not. We document the degree of this inconsistency, its correlates, and its implications, focusing primarily on the politics that congregants are exposed to from clergy and the attitudes they hold about salient political matters. The identity‐inconsistent attenders often vary significantly from identity‐consistent attenders, which serves to introduce considerable measurement error in the use of a religious tradition measure to depict American religion. The results suggest that salient disagreement induces a sizable population to migrate to a congregation outside their religious identity.
Diagnostic performance of transient elastography in differentiation between porto‐sinusoidal vascular liver disease and compensated cirrhosis
Authors: Zhang Guanhua , Ma Lin , Fu Li , Li Min , He Fuliang , Feng Lijuan , Wang Min , Jia Jidong , Wang Yu , Zhao Xinyan 
Published: August 2023

AbstractBackground and AimsThe efficacy of transient elastography (TE) in the differential diagnosis between porto‐sinusoidal vascular disease (PSVD) and compensated cirrhosis has not been sufficiently studied. We aimed to investigate the diagnostic performance of TE and identify histological lesions associated with liver stiffness.MethodsWe conducted a retrospective cohort study including patients with PSVD and cirrhosis (Child‐Turcotte‐Pugh class A) and healthy subjects. Both the PSVD and cirrhotic patients had at least one sign of PH. The area under the receiver operating characteristic curve (AUROC) was used for differentiation.ResultsNinety‐two patients with PSVD (median age: 53 years, 33% male), 100 patients with compensated cirrhosis and 101 healthy subjects were included. The median TE‐LSM in the PSVD patients (10.0 [7.0–13.0] kPa) was significantly lower than that in the cirrhotic patients (21.0 [15.0–28.0] kPa, p < .001) but was significantly higher than that in the healthy subjects (5.1 [4.6–6.0] kPa, p < .001). The AUROCs of TE‐LSM for the discrimination of PSVD from the cirrhosis and healthy subjects were 0.886 (95% CI: 0.833–0.928) and 0.913 (95% CI: 0.864–0.949), respectively. The sensitivity and specificity to discriminate PSVD from compensated cirrhosis were 78.3% and 82.0%, respectively, at a cut‐off of 13.6 kPa. Furthermore, portal fibrosis and aberrant cytokeratin 7 expression of centrilobular hepatocytes were significantly associated with higher TE‐LSM (≥10.0 kPa).ConclusionTE‐LSM can be used to differentiate PSVD from compensated cirrhosis. Pathological features in association with increased liver stiffness are identified.
Validity evidence supporting clinical skills assessment by artificial intelligence compared with trained clinician raters
Authors: Johnsson Vilma , Søndergaard Morten Bo , Kulasegaram Kulamakan , Sundberg Karin , Tiblad Eleonor , Herling Lotta , Petersen Olav Bjørn , Tolsgaard Martin G. 
Published: August 2023

AbstractBackgroundArtificial intelligence (AI) is becoming increasingly used in medical education, but our understanding of the validity of AI‐based assessments (AIBA) as compared with traditional clinical expert‐based assessments (EBA) is limited. In this study, the authors aimed to compare and contrast the validity evidence for the assessment of a complex clinical skill based on scores generated from an AI and trained clinical experts, respectively.MethodsThe study was conducted between September 2020 to October 2022. The authors used Kane's validity framework to prioritise and organise their evidence according to the four inferences: scoring, generalisation, extrapolation and implications. The context of the study was chorionic villus sampling performed within the simulated setting. AIBA and EBA were used to evaluate performances of experts, intermediates and novice based on video recordings. The clinical experts used a scoring instrument developed in a previous international consensus study. The AI used convolutional neural networks for capturing features on video recordings, motion tracking and eye movements to arrive at a final composite score.ResultsA total of 45 individuals participated in the study (22 novices, 12 intermediates and 11 experts). The authors demonstrated validity evidence for scoring, generalisation, extrapolation and implications for both EBA and AIBA. The plausibility of assumptions related to scoring, evidence of reproducibility and relation to different training levels was examined. Issues relating to construct underrepresentation, lack of explainability, and threats to robustness were identified as potential weak links in the AIBA validity argument compared with the EBA validity argument.ConclusionThere were weak links in the use of AIBA compared with EBA, mainly in their representation of the underlying construct but also regarding their explainability and ability to transfer to other datasets. However, combining AI and clinical expert‐based assessments may offer complementary benefits, which is a promising subject for future research.
Comparison of cardiac biomarkers on risk assessment of contrast‐associated acute kidney injury in patients undergoing cardiac catheterization: A multicenter retrospective study
Authors: Yu Sijia , Li Qiang , He Yibo , Jia CongZhuo , Liang Guoxiao , Lu Hongyu , Wu Wanying , Liu Jin , Liu Yong , Chen Jiyan 
Published: August 2023

AbstractAimCardiac biomarkers' predictive value of contrast‐associated acute kidney injury (CA‐AKI) remains unclear. We analysed whether creatine kinase isoenzyme‐MB (CKMB), cardiac troponin I (cTnI) and preoperative N‐terminal pro‐brain natriuretic peptide (NT‐proBNP) are tied to CA‐AKI patients undergoing cardiac catheterization.MethodsIn the multi‐center study, we included 3553 people underwent cardiac catheterization for analysis. CA‐AKI was defined as the absolute increase of over 0.3 mg/dL or an increase of more than 50% compared with the baseline serum creatinine within 48 hours following cardiac catheterization. Logistic regression model and receiver operating characteristic (ROC) curves were used to examine the association between cardiac biomarkers and CA‐AKI and the efficacy of Mehran risk score (MRS) model on CA‐AKI prediction with and without cardiac biomarkers.ResultsAmong 3553 people, 200 people eventually developed CA‐AKI. The logistic regression model showed that log10CKMB (odds ratio (OR): 1.97, 95%CI:1.51–2.57, p < .001), cTnI (OR: 1.03, 95%CI: 1.02–1.04, p < .001) and log10NT‐proBNP (OR: 3.19, 95%CI: 2.46–4.17, p < .001) were independent predictors of CA‐AKI. The ROC curve demonstrated that area under the curve (AUC) of MRS was 0.733. CKMB, cTnI and NT‐proBNP all significantly improved the AUC value in combination with MRS model. (NT‐proBNP: 0.798, p < .001; CKMB: 0.758, p = .003; cTnI: 0.755, p = .002), among which the NT‐proBNP had the best predictive efficacy improvement.ConclusionCardiac biomarkers of CKMB, cTnI and NT‐proBNP are all independently associated with CA‐AKI among patients undergoing cardiac catheterization while NT‐proBNP remains the best indicator. Adding CKMB, cTnI and NT‐proBNP to MRS improved the prognostic efficacy and may be considered effective tools to predict the risk of CA‐AKI in clinical practice.
Barriers and enablers for safe medication administration in adult and neonatal intensive care units mapped to the behaviour change wheel
Authors: Dick‐Smith Felicity , Fry Margaret Fry , Salter Rachel , Tinker Matthew , Leith Grace , Donoghoe Stephanie , Harris Claire , Murphy Sandra , Elliott Rosalind 
Published: August 2023

AbstractBackgroundIntensive care settings have high rates of medication administration errors. Medications are often administered by nurses and midwives using a specified process (the ‘5 rights’). Understanding where medication errors occur, the contributing factors and how best practice is delivered may assist in developing interventions to improve medication safety.AimsTo identify medication administration errors and context specific barriers and enablers for best practice in an adult and a neonatal intensive care unit. Secondary aims were to identify intervention functions (through the Behaviour Change Wheel).Study DesignA dual methods exploratory descriptive study was conducted (May to June 2021) in a mixed 56‐bedded adult intensive care unit and a 6‐bedded neonatal intensive care unit in Sydney, Australia.Incident monitoring data were examined. Direct semi‐covert observational medication administration audits using the 5 rights (n = 39) were conducted. Brief interviews with patients, parents and nurses were conducted. Data were mapped to the Behaviour Change Wheel.ResultsNo medication administration incidents were recorded. Audits (n = 3) for the neonatal intensive care unit revealed no areas for improvement. Adult intensive care unit nurses (n = 36) performed checks for the right medication 35 times (97%) and patient identity 25 times (69%). Sixteen administrations (44%) were interrupted. Four themes were synthesized from the interview data: Trust in the nursing profession; Availability of policies and procedures; Adherence to the ‘5 rights’ and departmental culture; and Adequate staffing. The interventional functions most likely to bring about behaviour change were environmental restructuring, enablement, restrictions, education, persuasion and modelling.ConclusionsThis study reveals insights about the medication administration practices of nurses in intensive care. Although there were areas for improvement there was widespread awareness among nurses regarding their responsibilities to safely administer medications. Interview data indicated high levels of trust among patients and parents in the nurses.Relevance to clinical practiceThis novel study indicated that nurses in intensive care are aware of their responsibilities to safely administer medications. Mapping of contextual data to the Behaviour Change Wheel resulted in the identification of Intervention functions most likely to change medication administration practices in the adult intensive care setting that is environmental restructuring, enablement, restrictions, education, persuasion and modelling.
Two functional CC‐NBS‐LRR proteins from rye chromosome 6RS confer differential age‐related powdery mildew resistance to wheat
Authors: Han Guohao , Liu Hong , Zhu Shanying , Gu Tiantian , Cao Lijun , Yan Hanwen , Jin Yuli , Wang Jing , Liu Shiyu , Zhou Yilin , Shi Zhipeng , He Huagang , An Diaoguo 
Published: August 2023

SummaryRye (Secale cereale), a valuable relative of wheat, contains abundant powdery mildew resistance (Pm) genes. Using physical mapping, transcriptome sequencing, barley stripe mosaic virus‐induced gene silencing, ethyl methane sulfonate mutagenesis, and stable transformation, we isolated and validated two coiled‐coil, nucleotide‐binding site and leucine‐rich repeat (CC‐NBS‐LRR) alleles, PmTR1 and PmTR3, located on rye chromosome 6RS from different triticale lines. PmTR1 confers age‐related resistance starting from the three‐leaf stage, whereas its allele, PmTR3, confers typical all‐stage resistance, which may be associated with their differential gene expression patterns. Overexpression in Nicotiana benthamiana showed that the CC, CC‐NBS, and CC‐LRR fragments of PMTR1 induce cell death, whereas in PMTR3 the CC and full‐length fragments perform this function. Luciferase complementation imaging and pull‐down assays revealed distinct interaction activities between the CC and NBS fragments. Our study elucidates two novel rye‐derived Pm genes and their derivative germplasm resources and provides novel insights into the mechanism of age‐related resistance, which can aid the improvement of resistance against wheat powdery mildew.