bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myostatin silencing inhibits podocyte apoptosis in membranous nephropathy through Smad3/PKA/NOX4 signaling pathway
Authors: Wang Juan , Shang Bangjuan , Tang Li , Tian Min , Liu Junping 
Published: March 2023

Abstract
This article focuses on deciphering the effect of myostatin (MSTN) on podocyte apoptosis in membranous nephropathy (MN) and fathoming out its underlying mechanism. Rats received the intravenous injection of cationized-bovine serum albumin to induce MN in vivo, while angiotensin II (Ang II) was exposed to AB8/13 cells to induce MN model in vitro. The mRNA expression of MSTN was detected by qRT-PCR. The effects of MSTN silencing on MN model rats and cells were assessed by cell counting kit-8 assay, flow cytometry, hematoxylin and eosin staining, and TUNEL assay. The expressions of proteins related to apoptosis and Smad3/protein kinase A (PKA)/NADPH oxidase 4 (NOX4) signaling pathway were examined by western blot. As a result, MSTN was highly expressed in MN cell and rat models. Besides, knockdown of MSTN elevated the MN cell viability and dwindled apoptosis rate, as well as attenuated kidney injury in MN rats. Meanwhile, MSTN silencing lessened the expressions of phosphorylated (p)-Smad3 and Nox4, while boosting the p-PKA expression in MN rats and cells. Additionally, Smad3 overexpression reversed the above effects of MSTN silencing on Ang II-induced podocytes. In conclusion, MSTN knockdown restrains the podocyte apoptosis through regulating Smad3/PKA/NOX4 signaling pathway.
Research progress and prospect of silica-based polymer nanofluids in enhanced oil recovery
Authors: Pan Yi , Zhang Changqing , Yang Shuangchun , Liu Yapeng , Muhammad Abbas 
Published: March 2023

Abstract
In recent years, silica-based polymer nanofluids (NFs) have attracted more and more attention because they can enhance temperature and salt tolerance. This study summarized the research progress and prospects of silica-based polymer NFs in enhanced oil recovery (EOR). First, the synthesis method and the effect on silica-based polymer NFs were studied. Research progress in rheology, wettability, viscoelasticity, interfacial tension, adsorption, porous media flow, and emulsion stability were reviewed, and the mechanism for EOR was expounded. Then, the mechanical properties of silica-based polymer NFs and the interaction between silica and polymer were discussed by molecular dynamics simulation. And the progress of research on macroscopic reservoir simulation was explored. Finally, the related auxiliary technologies were introduced, and future research directions were put forward. The results of this study will help researchers better understand the mechanism and research progress of silica-based polymer NFs in EOR.
The influence of preparation of nano-ZrO2/α-Al2O3 gradient coating on the corrosion resistance of 316L stainless steel substrate
Authors: Zhang Lianzhi , Wu Zhangyong , Wang Tingyou , Mo Ziyong 
Published: March 2023

Abstract
Generally, 316L stainless steel instrumentation tubes working in a humid environment with a large amount of Cl− all the year round have serious corrosion problems, so the stainless steel substrate should be gradiently coated with nano-ZrO2/α-Al2O3 slurry. In this article, the slender 316L stainless steel tube was first ground by magnetorheological fluid and then coated with the slurry, which can not only increase the contact area between the coating and the substrate but also prevent the generation of new substances that have adversely affected the adhesion of the coating. The properties of the samples were characterized and analyzed; the results showed that the substrate ground by magnetorheological fluid is more favorable for bonding with coating under the grinding conditions that the speed of the tube is 210 rpm, magnetic induction intensity is 40.83 mT, and mass ratio of micron and submicron magnetic particles is 2.3. The coating prepared under the above conditions has uniform thickness, flat surface, and can better inhibit the diffusion of Cr of the substrate to its surface. It can be obtained from corrosion resistance analysis that the coating has the highest self-corrosion potential of −0.016 V and the lowest corrosion current density of 0.491 μA/cm2, which indicate that the coating has the strongest corrosion resistance. According to the composition analysis of the coating, the composition of the corroded coating is similar to that of the coating itself, but accompanied by a small amount of Fe, which indirectly indicates that the coating is relatively compact, the coating is well bonded with the substrate, and the coating can protect the substrate; thus, the service life of 316L stainless steel instrumentation tubes is extended.
Optimierung optischer Absolutdrehwinkelmessungen durch verbesserte Codierungen
Authors: Janson Niels , Abt Marvin , Küster Benjamin , Overmeyer Ludger 
Published: March 2023

Zusammenfassung
Die optische Absolutdrehwinkelmessung ermöglicht die kontaktlose und referenzfreie Messung von Winkelpositionen an rotierenden Wellen, ohne dass zusätzliche Aufbauten oder Verkabelungen an diesen angebracht werden müssen. Neben den klassischen Methoden zur Steigerung der Robustheit und Effizienz des Messverfahrens durch optimierte Hardware, Messdatenverarbeitung oder Fehlerkorrekturen stellt die Auseinandersetzung mit der zugrundeliegenden Codierung einen vielversprechenden und bisher wenig betrachteten Ansatz dar. Im Rahmen dieser Arbeit wird anhand einer realen Welle gezeigt, wie durch die Verwendung geeigneter Suchverfahren und einer angepassten Codierung die erforderliche Codewortlänge um über 40 % verkürzt werden kann. Dadurch wird die Rechenlast und der Speicherbedarf für die Dekodierung deutlich verringert und die Robustheit des Verfahrens gesteigert. Weiterhin wird praktisch gezeigt, wie durch Verkürzung der Codewortlänge eingesparten Stellen genutzt werden können, um Messfehler durch eine zusätzliche Redundanz effektiv zu minimieren.
Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis
Authors: Ju Jia‐Qian , Pan Zhen‐Nan , Zhang Kun‐Huan , Ji Yi‐Ming , Liu Jing‐Cai , Sun Shao‐Chen 
Published: March 2023

AbstractMicrospherule protein 1 (Mcrs1) is a component of the nonspecific lethal (NSL) complex and the chromatin remodeling INO80 complex, which participates in transcriptional regulation during mitosis. Here, we investigate the roles of Mcrs1 during female meiosis in mice. We demonstrate that Mcrs1 is a novel regulator of the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes of oocytes during meiosis I. Depletion of Mcrs1 alters HDAC2‐mediated H4K16ac, H3K4me2, and H3K9me2 levels in nonsurrounded nucleolus (NSN)‐type oocytes, and reduces CDK1 activity and cyclin B1 accumulation, leading to G2/M transition delay. Furthermore, Mcrs1 depletion results in abnormal spindle assembly due to reduced Aurora kinase (Aurka and Aurkc) and Kif2A activities, suggesting that Mcrs1 also plays a transcription‐independent role in regulation of metaphase I oocytes. Taken together, our results demonstrate that the transcription factor Mcrs1 has important roles in cell cycle regulation and spindle assembly in mouse oocyte meiosis.
Newton-GMRES-Method for the Scritinization of electric double layer and surface roughness on EHL line contact problem
Authors: Awati Vishwanath B , Obannavar Parashuram , N Mahesh Kumar 
Published: March 2023

The electric double layer phenomenon exists on the solid interface under the water-liquid condition. The water molecules are ionized and adhered in the interface forming the sturn layer is a diffused layer in which molecules can move with the movement of bulk of molecules. Because of these two characteristics, the boundary layer of water molecules is called the electric double layer. The aim of present study is to explore the impact of two fraction surfaces of electric double layer (EDL) on a thin water lubricating film on an elastohydrodynamic lubrication (EHL) line contact problem with a sinusoidal surface roughness. The governing modified Reynolds and film thickness equations are based on mathematical model of electro-viscosity of asymmetrical electrical double layer is analyzed numerically. The viscosity-pressure relation of water and theoretical evaluation pertaining to the effect of electric double layer on film-thickness and pressure distribution of EHL with water film of line contact problem is discussed in detail. The effect of zeta potential on film thickness and pressure is determined using Newton’s-GMRES method with Daubechies D6 wavelet as a pre-conditioner. The results predict that, EDL has less impact on pressure distribution and significant impact on film thickness. The obtained results are compared with results of Dowson and Higginson which are comparable.
Kompetencije učitelja potrebne za provedbu sata Tjelesne i zdravstvene kulture
Authors: Alić Jelena , Petković Nina , Ivković Gordana 
Published: March 2023

Kompetencije učitelja u razrednoj nastavi za tjelesno i zdravstveno odgojno-obrazovno područje dijele se na generičke i predmetno specifične. Generičke kompetencije odnose se na opće kompetencije, a obuhvaćaju teorijska i praktična znanja, dok se predmetno specifične kompetencije odnose na primjenu spoznaja supstratne znanosti predmetnog područja u cilju izvršavanja postavljenih zadaća. U dosadašnjim istraživanjima utvrđene su statistički značajne razlike između procjene razine kompetencija učitelja i studenata. Cilj ovog istraživanja bio je utvrditi razinu kompetencija učitelja i studenata koje su potrebne za provođenje sata Tjelesne i zdravstvene kulture (TZK) te utvrditi razlike u njihovoj razini procijenjenih kompetencija. Istraživanje je provedeno s učiteljima osnovnih škola u Dubrovniku i studentima pete godine Odjela za izobrazbu učitelja i odgojitelja. Razina kompetencija procijenjena je indirektno primjenom hrvatske verzije upitnika Kompetencije u Tjelesnom i zdravstvenom području (Competencies in Physical Education Teaching, Al-Tawel i AlJaafreh, 2017). Rezultati istraživanju pokazali su kako učitelji svoje ukupne kompetencije procjenjuju izrazito visokima (AS = 4.52), a studenti visokima (AS = 4.23). Vezano za subskale upitnika, dobiven je rezultat da učitelji visokima procjenjuju svoje kompetencije za provedbu (AS = 4.34) i planiranje sata TZK-a (AS = 4.30), a izrazito visokima procjenjuju motivacijske (AS = 4.85) te osobne i socijalne kompetencije (AS = 4.60). Studenti procjenjuju visokima svoje kompetencije provedbe (AS = 4.22), kompetencije planiranja (AS = 4.00) te osobne i socijalne kompetencije (AS = 4.17), a izrazito visokima procjenjuju motivacijske kompetencije (AS = 4.51). Testiranjem razlika utvrđeno je kako postoje statistički značajne razlike u tri subskale: kompetencijama potrebnim za provođenje sata TZK-a, motivacijskim kompetencijama te osobnim i socijalnim kompetencijama. Učitelji razinu svojih kompetencija za te tri subskale procjenjuju višima u odnosu na studente. Nadalje, istraživanjem je utvrđeno kako ispitanici dijele mišljenje da bi tijekom učiteljskog studija trebalo uvesti više prakse iz kineziološkog područja kako bi se poboljšale i unaprijedile njihove kompetencije. Rezultati istraživanja mogu pomoći u osmišljavanju učinkovitijeg plana i programa Učiteljskih studija usmjerenih poboljšanju kompetencija koje će steći budući učitelji razredne nastave.
Intracellular FGF1 promotes invasion and migration in thyroid carcinoma via HMGA1 independent of FGF receptors
Authors: Chen Zuyao , Zhong Xiaolin , Tang Weiqiang , Xia Min , Liu Chang , Guo Yinping , Yi Yan , Jiang Qingshan , Zu Xuyu , Zhong Jing 
Published: March 2023

Background
Fibroblast growth factor 1 (FGF1) is extensively amplified in many tumors and accelerates tumor invasion and metastasis. However, the role and precise molecular mechanism by which FGF1 participates in thyroid cancer (TC) are still unclear.

Methods
Quantitative real-time polymerase chain reaction- and western blotting were used to detect the mRNA and protein levels of FGF1, high mobility group A (HMGA1), epithelial-to-mesenchymal transition (EMT)-related factors, and FGFs in both TC tissues and cell lines. Immunohistochemistry was conducted to examine the expression of FGF1 and HMGA1. Immunofluorescence staining was used to detect the coexpression of FGF1 and HMGA1. Transwell and wound healing assays were conducted to evaluate the effects of FGF1 on the capacity of invasion and migration in cells.

Results
FGF1 was upregulated in papillary thyroid carcinoma (PTC) tissues and cell lines and was relatively higher in PTC tissues with cervical lymph node metastasis. Furthermore, FGF1 promotes invasion and metastasis through the EMT pathway. Mechanistically, FGF1 promotes EMT through intracellular function independent of FGF receptors. Interestingly, we demonstrated that FGF1 could upregulate HMGA1 in TC cells, and the correlation of FGF1 and HMGA1 was positive in PTC tissues. FGF1 and HMGA1 had obvious colocalization in the nucleus. We further revealed that FGF1 promotes the invasion and migration of TC cells through the upregulation of HMGA1.

Conclusion
Intracellular FGF1 could promote invasion and migration in TC by mediating the expression of HMGA1 independent of FGF receptors, and FGF1 may be an effective therapeutic target in TC.
The associative learning roots of affect-driven impulsivity and its role in problem gambling: A replication attempt and extension of Quintero et al. (2020)
Authors: Muela Ismael , Ventura-Lucena José M. , Navas Juan F. , Perales José C. 
Published: March 2023

AbstractBackground and aimsNegative/positive urgency (NU/PU) refers to the proneness to act rashly under negative/positive emotions. These traits are proxies to generalized emotion dysregulation, and are well-established predictors of gambling-related problems. We aimed to replicate a previous work (Quintero et al., 2020) showing NU to be related to faulty extinction of conditioned stimuli in an emotional conditioning task, to extend these findings to PU, and to clarify the role of urgency in the development of gambling-related craving and problems.Methods81 gamblers performed an acquisition-extinction task in which neutral, disgusting, erotic and gambling-related images were used as unconditioned stimuli (US), and color patches as conditioned stimuli (CS). Trial-by-trial predictive responses were analyzed using generalized linear mixed-effects models (GLME).ResultsPU was more strongly related than NU to craving and severity of gambling problems. PU did not influence acquisition in the associative task, whereas NU slightly slowed it. Extinction was hampered in individuals with high PU, and a follow-up analysis showed this effect to depend on relative preference for skill-based and casino games.Discussion and conclusionsResults suggest that resistance to extinction of emotionally conditioned cues is a sign of malfunctioning emotion regulation in problematic gambling. In our work, the key effect was driven by PU (instead of NU), and gambling craving and symptoms were also more closely predicted by it. Future research should compare the involvement of PU and NU in emotion regulation and gambling problems, for gamblers with preference for different gambling modalities (e.g., pure chance vs skill games).
Análise da Produção Acadêmica sobre Estética Organizacional
Authors: Assi Ilton Luiz 
Published: March 2023

O objetivo deste ensaio é de apresentar o processo evolutivo da temática estética organizacional, seu esforço em proporcionar maior e melhor ambiência organizacional – “do insosso ou rígido ao saboroso e flexível” – aumentando a beleza ambiental, visual, emocional e sensitiva da organização. Assim como as organizações são produtoras de novas experiências, e que imputa desejo de estar presente e fazer parte, possui-la. Retrocedemos em busca da base conceitual da estética organizacional e caminhamos até a atualidade. É realizada uma ampla revisão da literatura aplicando rigor e sistematização do método PRISMA. É relevante minimiza a fragmentação do tema, em uma sequência de construção acadêmica lógica e consistente. Essa consistência tende a ajudar interessados na pesquisa da temática. Por fim, conclui-se que a estética organizacional é fundamental para o bem-estar, sustentabilidade, identidade, aplicação prática contextos complexos e conflitantes.
The Utilization of Memrise in Arabic Language Learning with Blended Learning Method
Authors: Subita Aulia , Ahsanuddin Mohammad 
Published: March 2023

Rapid development of technology influences learning process where online learning has replaced the traditional learning. However, because of the numerous problems posed by this transition, the learning process has changed to use blended learning by utilizing technological media. This research aims to describe the utilization of Memrise in Arabic language learning with blended learning method and to describe the obstacles in utilizing Memrise in learning process. This study employs a descriptive qualitative methodology with Miles-Huberman’s data analysis techniques. This study revealed that the process of utilizing Memrise in Arabic language learning using the blended learning method is conducted through several stages, which are preparing the media, face-to-face learning, and online learning. The obstacles in utilizing Memrise in learning process faced by the teacher and students, including inadequate facilities, extra payment for more features and less flexible quizzes.
trabalho de coordenação pedagógica em instituições públicas de Educação Infantil
Authors: Bezerra Machado Laeda , LOPES FRANÇA ELAINE 
Published: March 2023

Este artigo procura caracterizar a atuação da coordenação pedagógica em instituições de educação infantil, enfatizando seu papel junto à equipe gestora, professores, alunos e famílias dessas instituições. Tratamos conceitualmente coordenação pedagógica, seus aspectos legais e produção cientifica sobre o tema. Desenvolvemos uma pesquisa de abordagem qualitativa, delineada como estudo de campo, com oito coordenadoras que atuam em instituições municipais de educação infantil, situadas no Recife-PE. Os resultados sugerem uma relação de mediação e parceria entre coordenação pedagógica, equipe gestora, docentes, crianças e famílias. No âmbito da coordenação pedagógica detectamos dificuldades para a atuação dessas profissionais em processos de formação continuada de professores dessas instituições.
Method for creating a recombinant strain of enterococcus L3-SARS based on biologically active strain Enterococcus faecium L3
Authors: Bormotova E. A. , Gupalova T. V. , Suvorov A. N. 
Published: March 2023

The current pandemic caused by the SARS-Cov-2 virus has significantly influenced the emergence of new injectable vaccines that provide a predominantly specific IgG response. However, it is generally accepted that protection against pathogens at the mucosal surface, which is the first barrier to viral entry, is predominantly dependent on the IgA response. It is now widely accepted that the use of genetically modified microorganisms, including probiotics, allows the oral or nasal mucosal delivery of therapeutic molecules, inducing an immune response in the mucous membranes. Probiotic strains are well studied for safety for the organism and are able to remain viable after passing through the gastric barrier, improve intraepithelial connections, and can generate a number of surface expressed molecules that enhance the effectiveness of vaccination.Recombinant probiotic microorganisms capable of producing vaccine antigens by inserting specific DNA fragments into their genome are one of the potential platforms that can be used to develop an appropriate vaccine containing a specific antigen for rapid response to viral mutations. Here, we demonstrate the construction of a novel SARS-Cov-2 vaccine candidate employing the gene fragment of S1 SARS-Cov-2 gene. According to the available data on new variants of SARS-Cov-2 mutations, three amino acid substitutions were made in the chosen sequence. This DNA fragment was inserted in frame into major pili protein gene within d2 domain of enterococcal operon encoding for pili.
Use of autoprobiotics in the complex therapy of axial spondyloarthritis
Authors: Artemev I. A. , Ermolenko E. I. , Kotyleva M. P. , Gladysheva N. P. , Tsapieva A. N. , Gaydukova I. Z. , Chudinov A. L. , Suvorov A. N. , Maslyansky A. L. 
Published: March 2023

Spondyloarthritis (SpA) is a group of chronic inflammatory diseases of the musculoskeletal system involving of the axial skeleton and extra-articular manifestations such as inflammatory bowel diseases. Some violations of the intestinal microbiome often occur during the course of spondyloarthritis. Also, intestinal dysbiosis can be enhanced by ongoing therapy. The aim of the study was to evaluate the effectiveness of combined therapy with nonsteroidal anti-inflammatory drugs (NSAIDs) and autoprobiotics supplementation.SpA patients treated with NSAID were divided into two groups: group A which took autoprobiotic based on indigenous culture of Enterococcus faecium, and group S which took only Supra medium, which is the basis used for making of autoprobiotic. Reducing of pain intensity, dyspeptic phenomena were observed to a greater extent in group A compared to group S. PCRRT testing revealed no significant changes in intestinal microbiocenosis in patients with SpA, except of a decrease in the Lactobacillus population, which was restored only in group A. A feature of the changes in group S was a decrease in the total bacterial mass, amounts of Bacteroides, Faecalibacterium, Enterobacter and expansion of Methanobrevibacter population. The tendency to restore the quantitative content of Lactobacillus, correlating with a decrease of IL-10 concentration, was found only in group A.In our study the effectiveness of enterococcal auprobiotic supplementation as an element of complex therapy of patient suffering from SpA has been proven. The use of an autoprobiotic leads to a decrease in the severity of the symptoms of the disease, the leveling of dyspeptic symptoms and microbiota disorders.
Artificial Intelligence based Optimized Resource Allocation using Predictive Analysis in a Virtual Environment
Authors: B Sahana , Dinesh Diksha , N Deekshith K , Sru A V , Deshpande Abhay , Priyadarshi Rahul , Nath Vijay 
Published: March 2023

Abstract
In a virtual environment, load balancing is the best method of assigning a collection of tasks through a range of resources, in order to make the total output more efficient. Load balancers not only improve efficiency but also ensure system stability. Resource allocation in a virtual environment in this article is accomplished by using Amazon Web Services (AWS) and machine learning techniques. Round Robin resource allocation algorithm is used by HAProxy to perform Load Balancing responsibilities. The virtual servers created through Amazon Elastic Compute Cloud (EC2), all have the same characteristics, such as load bearing capacity and reaction time. One of the EC2 instances created is configured to host the HAProxy. The load bearing potential of HAProxy is inversely proportional to the execution time, ensuring faster processing of the incoming requests. HAProxy, as the load balancer, optimizes resource allocation and provides flexibility in contrast to a standard application load balancer. The logs created by the instances help predict future load on the servers, by applying the ARIMA time-series forecasting model. A Mean Squared Error of 3.28 is obtained, justifying the accuracy of the prediction.