bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hox genes are crucial regulators of periosteal stem cell identity
Authors: Leclerc Kevin , Remark Lindsey H. , Ramsukh Malissa , Josephson Anne Marie , Palma Laura , Parente Paulo E. L. , Sambon Margaux , Lee Sooyeon , Lopez Emma Muiños , Morgani Sophie M. , Leucht Philipp 
Published: March 2023

ABSTRACT
Periosteal stem and progenitor cells (PSPCs) are major contributors to bone maintenance and repair. Deciphering the molecular mechanisms that regulate their function is crucial for the successful generation and application of future therapeutics. Here, we pinpoint Hox transcription factors as necessary and sufficient for periosteal stem cell function. Hox genes are transcriptionally enriched in periosteal stem cells and their overexpression in more committed progenitors drives reprogramming to a naïve, self-renewing stem cell-like state. Crucially, individual Hox family members are expressed in a location-specific manner and their stem cell-promoting activity is only observed when the Hox gene is matched to the anatomical origin of the PSPC, demonstrating a role for the embryonic Hox code in adult stem cells. Finally, we demonstrate that Hoxa10 overexpression partially restores the age-related decline in fracture repair. Together, our data highlight the importance of Hox genes as key regulators of PSPC identity in skeletal homeostasis and repair.
Sex-related differences in symptom presentation of patients with aneurysmal subarachnoid hemorrhage
Authors: Westphal Laura Philine , Bögli Stefan Yu , Werner Jana , Casagrande Francesca , Keller Emanuela , Brandi Giovanna 
Published: March 2023

Background: In patients with myocardial infarction, atypical symptoms at onset have been demonstrated in women. We aimed to investigate the presence of sex-related differences in symptom presentation in patients with aneurysmal subarachnoid hemorrhage (aSAH) to enable earlier diagnosis and treatment. Methods: We assessed symptoms on admission to hospital in 343 patients with aSAH in this retrospective single-center cohort-study. Univariate statistical analysis was performed by comparing sexes including the whole study population and subgroups (dichotomized using Fisher scale 1-2 vs. 3-4, WFNS grade 1-3 vs. 4-5, and anterior vs. posterior circulation aneurysms, respectively). Results: The majority of patients was female (63.6%, n=218, vs. 36.4%, n=125), the mean age 57.4 years (standard deviation (SD) 13.3) with older women compared to men (59.2, SD 13.8, vs. 54.4, SD 11.6; p=0.003). Anterior communicating artery (AcomA) aneurysms were most common (30.9%, n=106), predominantly in men (43.2%, n=54, vs. 23.9%, n=52; p=0.0002), whereas posterior communicating artery (PcomA) aneurysms were more frequent in women (19.3%, n=42, vs. 8.8%, n=11; p=0.005). Exercise-induced headache was more often reported by men (10.4%, n=13, vs. 5%, n=11; p=0.04) in all patients as well as in the subgroup of WFNS 1-3. Anisocoria was more frequent in women within the subgroup of severely impaired consciousness (WFNS 4-5; 25.3%, n=22, vs. 10.7%, n=6; p=0.032). For all other symptoms, there was no evidence for sex-specific differences in the whole study group as well as in subgroups. Conclusion: Our results show no evidence for relevant sex-related differences in symptom presentation at onset in aSAH patients. Women presenting with an acute onset anisocoria should be screened even more carefully for an underlying ruptured Pcom aneurysm.
Seroepidemiology of Hepatitis E virus among the voluntary blood donors in the coastal region of Karnataka, India
Authors: S Sangthang , Shastry Shamee , N Sudheesh , Chawla Kiran , Madiyal Mridula , Kandasamy Dhivya 
Published: March 2023

Background: Hepatitis E is a fecal orally transmitted disease and an emerging transfusion transmissible infection with potential transfusion safety implications. Hepatitis E Virus screening among blood donors is not routinely done despite the fact that it is endemic in India. As there are very few reports and evidence, the justification for mandating HEV screening among blood donors is still only marginally convincing. Therefore, the present study was carried out to determine the incidence and prevalence of HEV infection among blood donors in Coastal Karnataka, India. Methods: This cross-sectional study was conducted in a tertiary care hospital in Udupi district of Karnataka, India. A total of 1939 blood donors were randomly selected between June 2020 to June 2022 to participate in the study, where anti-HEV IgM antibody screening was performed using HEV IgM ELISA test kit (DiaPro, Italy). Further, a randomly selected 588 and 1620 pooled donor samples were screened for anti-HEV IgG antibodies and HEV RNA, respectively. Results: The overall prevalence of HEV IgM antibodies among study application is found to be 1.39%, with a statistically significant difference between males and females (n=20, 1.18% vs. n=7, 3.07 %; p=0.027). The prevalence of HEV IgG antibodies was 11.39%. Exposure to agriculture, animal husbandry, and poor hand hygiene were significant risk factors for HEV IgG reactivity. Conclusions: Given the high prevalence of HEV viral markers in the study population, routine screening of blood donors for risk factors and implementation of serologic testing in endemic areas may be considered.
Evaluation of the dimensional changes in the mandible, condyles, and the temporomandibular joint following skeletal class III treatment with chin cup and bonded maxillary bite block using low-dose computed tomography: A single-center, randomized controlled trial
Authors: Husson Amr H. , Burhan Ahmad S. , Hajeer Mohammad Younis , Nawaya Fehmieh R. 
Published: March 2023

Background: Insufficient evidence regarding the effects of chincup therapy on the mandibular dimensions and temporomandibular joint (TMJ) structures requires high-quality studies using three-dimensional (3D) imaging. This trial aimed to evaluate the 3D changes in the mandible, condyles, and glenoid fossa after chin cup therapy for skeletal Class III children compared to untreated controls. Methods: A 2-arm parallel-group randomized controlled trial on 38 prognathic children (21 boys and 17 girls), with mean ages 6.63±0.84 years. Patients were recruited and randomized into two equal groups; the experimental group (CC) was treated with occipital-traction chin cups in conjunction with bonded maxillary bite blocks. No treatment was provided in the control group (CON). Low-dose CT images were acquired before (T1) and after achieving (2-4 mm) positive overjet (T2), and after 16 months apart in both groups. The outcome measures of the condyle-mandibular 3D distances, the condyles-glenoid fossa postional changes, and the quantitative displacement parameters of superimposed 3D models were compared statistically. Paired- and two-sample t-tests were used for intra- and inter-group comparisons, respectively. Results: Overall, 35 patients (18 and 17 in the CC and the CON groups, respetively) were enrolled in the statistical analysis. The mean mandibular and condylar volumes increased significantly by 777.24 mm3 and 1,221.62 mm3, 94.57 mm3, and 132.54 mm3 in the CC and CON groups, respectively. No statistically significant differences were observed between the groups regarding the volumes, superficial areas, and linear changes of the mandible and condyles, and part analysis measurements, except the changes of the relative sagittal and vertical positions of condyles, glenoid fossa, and posterior joint space, which were significantly smaller in the CC group (p<0.05) than the CON group. Conclusions: The chin cup did not affect the mandibular dimensions. Its primary action was confined to the condyles and the TMJ internal dimensions. Clinicaltrials.gov registration: NCT05350306 (28/04/2022).
Seasonal effects decouple SARS-CoV-2 haplotypes worldwide
Authors: Tomaszewski Tre , Ali Muhammad Asif , Caetano-Anollés Kelsey , Caetano-Anollés Gustavo 
Published: March 2023

Background: Variants of concern (VOCs) have been replacing each other during the still rampant COVID-19 pandemic. As a result, SARS-CoV-2 populations have evolved increasingly intricate constellations of mutations that often enhance transmissibility, disease severity, and other epidemiological characteristics. The origin and evolution of these constellations remain puzzling. Methods: Here we study the evolution of VOCs at the proteome level by analyzing about 12 million genomic sequences retrieved from GISAID on July 23, 2022. A total 183,276 mutations were identified and filtered with a relevancy heuristic. The prevalence of haplotypes and free-standing mutations was then tracked monthly in various latitude corridors of the world. Results: A chronology of 22 haplotypes defined three phases driven by protein flexibility-rigidity, environmental sensing, and immune escape. A network of haplotypes illustrated the recruitment and coalescence of mutations into major VOC constellations and seasonal effects of decoupling and loss. Protein interaction networks mediated by haplotypes predicted communications impacting the structure and function of proteins, showing the increasingly central role of molecular interactions involving the spike (S), nucleocapsid (N), and membrane (M) proteins. Haplotype markers either affected fusogenic regions while spreading along the sequence of the S-protein or clustered around binding domains. Modeling of protein structure with AlphaFold2 showed that VOC Omicron and one of its haplotypes were major contributors to the distortion of the M-protein endodomain, which behaves as a receptor of other structural proteins during virion assembly. Remarkably, VOC constellations acted cooperatively to balance the more extreme effects of individual haplotypes. Conclusions: Our study uncovers seasonal patterns of emergence and diversification occurring amid a highly dynamic evolutionary landscape of bursts and waves. The mapping of genetically-linked mutations to structures that sense environmental change with powerful ab initio modeling tools demonstrates the potential of deep-learning for COVID-19 predictive intelligence and therapeutic intervention.
Application of the Koch Curve to Increase the Strength of Aircraft Parts
Authors: Zhikharev L. 
Published: March 2023

Fractals are formed by iterative repetition of the construction algorithm at different scale levels. The use of such an algorithm, which increases the strength properties during the construction of the structure, will strengthen these properties with each iteration. The Koch curve principle is applied in the article. Replacing the compressible plate with four new ones connected at angles increases the stability of the structure.
This article theoretically confirms the increase in the stability of the Koch plate both at the level of individual plates and at the level of fractal segments and the structure as a whole (general stability). Regularities of stability changes at different scale levels with an increase in the number of iterations are established. A comparison of variants of Koch plates with different similarity coefficients is also carried out.
The theoretical results were confirmed using simulations in the CAE system Solid-Works - a finite element analysis of the stability of computer models of the Koch plates was carried out. The graphs constructed from the obtained data correspond to the theoretical predictions of the dependence of stability on the geometric parameters of the Koch plate.
As an illustration of the applicability of this kind of fractal structures in the design of aircraft parts, a fractal modification of a typical part, the slat rail, has been developed. The proposed modification of the rail was also investigated using computer simulations. A comparison of the strength properties of a standard-shaped part and its analogue with a fractal structure included showed the advantage of the latter: with certain values of mass and loading scheme, the fractal modification showed twice as much stability. This reduces the weight of the standard slat rail by 5% without loss of strength properties.
ROUTINE FIXATION OF HUMERAL SHAFT FRACTURES IS COST-EFFECTIVE: COST-UTILITY ANALYSIS OF 215 PATIENTS AT FIVE YEARS FOLLOWING INJURY
Authors: Oliver W.M. , Molyneux S.G. , White T.O. , Clement N.D. , Duckworth A.D. 
Published: March 2023

The primary aim was to estimate the cost-effectiveness of routine operative fixation for all patients with humeral shaft fractures. The secondary aim was to estimate the cost-effectiveness of using a Radiographic Union Score for HUmeral fractures (RUSHU)<8 to facilitate selective fixation for patients at risk of nonunion.From 2008-2017, 215 patients (mean age 57yrs [17–81], 61% female) with a non-operatively managed humeral diaphyseal fracture were retrospectively identified. Union was achieved in 77% (n=165/215) after non-operative management, with 23% (n=50/215) uniting after nonunion surgery. The EuroQol Five-Dimension (EQ-5D) Health Index was obtained via postal survey. An incremental cost-effectiveness ratio (ICER) <£20,000 per quality-adjusted life-year (QALY) gained was considered cost-effective.At a mean of 5.4yrs (1.2–11.0), the mean EQ-5D was 0.736. Multiple regression demonstrated that uniting after nonunion surgery was independently associated with an inferior EQ-5D (beta=0.103, p=0.032). Routine fixation for all patients to reduce the nonunion rate would be associated with increased treatment costs (£1,542/patient) but confer a potential EQ-5D benefit of 0.120/patient. The ICER of routine fixation was £12,850/QALY gained. Selective fixation, based upon a RUSHU<8 at 6wks post-injury, would be associated with reduced treatment costs (£415/patient) and confer a potential EQ-5D benefit of 0.335 per ‘at-risk patient’.Routine fixation for patients with humeral shaft fractures, to reduce the nonunion rate observed after non-operative management, appears to be cost-effective at 5yrs post-injury. Selective fixation for patients at risk of nonunion based upon the RUSHU may confer greater cost-effectiveness, given the potential savings and improvement in EQ-5D.
NATIVE KNEE KINEMATICS ARE REPRODUCED DURING THE GOLF SWING AFTER TOTAL KNEE ARTHROPLASTY: A CASE CONTROL STUDY OF HIP AND KNEE KINEMATICS OF PATIENTS RETURNING TO GOLF COMPARED TO MATCH CONTROLS
Authors: Rankin C.S. , Coleman S. , Robinson P.G. , Murray I.R. , Clement N.D. 
Published: March 2023

We aim to determine the differences in lower limb joint kinematics during the golf swing of patients who had undergone Total Knee Arthroplasty (TKA) and a control group of native knee golfers.A case-control study was undertaken with ten golfers who had undergone TKA (cruciate retaining single radius implant) and five age and matched golfers with native knees. Each golfer performed five swings with a driver whilst being recorded at 200Hz by a ten-camera motion capture system. Knee and hip three-dimensional joint angles (JA) and joint angular velocities (JAV) were calculated and statistically compared between the groups at six swing events.The only significant differences in knee joint kinematics between TKA and control groups was a lower external rotation JA in the left knee during the backswing (p=0.010). There was no significant difference in knee JAV between the groups. Both hips demonstrated significantly (p=0.023 for left and p=0.037 for right) lower flexion in the TKA group during the takeaway swing event, and there was lower internal rotation in the backswing and greater external rotation in the downswing of the right hip. There was also slower left hip extension JAV in the downswing.Normal knee kinematics were observed during the golf swing following TKA, with the exception of reduced external rotation in the left knee during the back swing and the right during the down swing. The differences demonstrated in the hip motion indicate that they may make compensatory movements to adjust to the reduced external rotation demonstrated in the knee.
Anti-Alzheimer, antioxidants, glucose-6-phosphate dehydrogenase effects of Taverniera glabra mediated ZnO and Fe2O3 nanoparticles in alloxan-induced diabetic rats
Authors: Khan Aziz Ullah , Mohany Mohamed , Khan Hidayat Ullah , Fozia Fozia , Khan Shahnaz , Kamran Naveed , Khan Fahim Ullah , Al-Rejaie Salim S. , Ahmad Ijaz , Zaghloul Nouf S. S. , Aboul-Soud Mourad A. M. 
Published: March 2023

Abstract
The current study aimed to assess the neuroprotective effect of Zn and Fe oxide nanoparticles biofabricated by Taverniera glabra in alloxan-induced diabetic rats. The experimental animals (160–200 g) were divided into nine groups (n = 9). The blood glucose, body weight, glucose-6-phosphate dehydrogenase (G6PD), superoxide dismutase (SOD), catalase test (CAT), lipid peroxidation (TBARS), glutathione (GSH), and acetylcholinesterase (AChE) activities were determined. Oral administration of nanoparticles and T. glabra methanol extract (TGME; 10 and 15 mg/kg b.w) significantly decreased the glucose level, increased the body weight, controlled the quantitative level of G6PD, and significantly decreased the levels of ALT, ALP, cholesterol, and creatinine. Moreover, TGME and their Zn and Fe oxide nanoparticles significantly restored the antioxidant enzymes (SOD, CAT, GPx, and GSH) that decreased during induced diabetes. In the diabetic group, a significant increase in TBARS was noted and recovered in diabetic animals (p < 0.05) as compared to glibenclamide. The AChE activity was significantly recovered by nanoparticles and TGME both in the blood and brain of the diabetic group (p < 0.05). Taken together, it can be suggested that TGME and Zn and Fe oxide nanoparticles significantly improved memory and could be considered as an effective biogenic nanomaterial for diabetes, Alzheimer’s disease, and oxidative stress.
On the role of time, re-presentation, and self-conscious narrators in postmodern narrative
Authors: Baker Baranna 
Published: March 2023

Abstract
In the past, I have published papers on the use of language within the realm of fictional narratives, how the structure of postmodern films and novels operates to affect the reader or viewer, and on the purely objective worlds constructed within the confines of literature. Most recently, I submitted a newly written paper to my friend Paul Cobley for his casual feedback. He got back promptly, saying only that the paper did not have enough references. I pressed Paul: Was it not possible to write a philosophy paper where one just philosophized, without alluding to things written on the subject matter in the past, and wasn’t it ok not to cite references if you came up with the idea independent of any outside reading? His reply was: “No.” Time passed, and I was asked to write this article celebrating Paul’s 60th birthday. Looking for something I hadn’t read already, I discovered his book, Narrative (2014). This paper addresses one of the reference entries most definitely missing from my prior investigations on literature and cinema, one which should have informed – and now does my own thoughts on fiction and film.
Narrative modeling and its implications for cultural practices
Authors: Lee Yunhee 
Published: March 2023

Abstract
Paul Cobley stated that the semiotics of narrative should not be conflated with narratology. This statement becomes a starting point for an inquiry into the semiotics of narrative by looking at the concept of narrative signs and its future as a new theory of narrative. Narrative signs embedding semiotic processes convey the meaning of narrative in the areas of the prelinguistic, the linguistic, and the extralinguistic by way of signs, models, and semiosis. What is more, the concept of narrative modeling for Cobley enables further inquiry into cultural activity through the act of narration for transvaluation. In this regard, a new theory of narrative involves time, emotion, abduction, and the dialogic self, leading to the narrative-related ideas of cognition, identity, and human subjectivity. Based on Peirce’s semiotics and a biosemiotic approach, narrative modeling makes human beings participate in sign activity, that is, cultural activity through dialogic interaction between culture and nature. Consequently, this paper proposes that the study of the mysterious narrative through narrative modeling is geared to seeing how it affects humans and also how they see and make a world through various cultural practices.
Semioethics and global communication
Authors: Petrilli Susan , Ponzio Augusto 
Published: March 2023

AbstractIn the sign of homaging Paul Cobley as part of thisFestschriftfor him, we will consider two of his edited volumes: the firstThe Routledge companion to semiotics, 2010, to which we contributed a text titled “Semioethics,” and the second (co-edited with Kristian Bankov),Semiotics and its masters, 2017, to which we contributed the text “Semioethics as a vocation of semiotics.” Particular reference is made to Paul’s observations in his “Introduction” to Part I, “Understanding semiotics,” in the 2010 book, and in his opening essay, “What the humanities are for – a semiotic perspective,” in Section 1: “Semiotics in the world and academia,” in the 2017 book. What follows is an ideal discussion with Paul regarding “Semioethics” and possible developments today. In line with critical semiotics, our focus is on communication in globalization and the need for education to dialogism, plurilingualism, and critique for a new humanism, a primary task for the humanities today.
Bernstein-type operators on elliptic domain and their interpolation properties
Authors: Iliyas Mohammad , Khan Asif , Mursaleen Mohammad 
Published: March 2023

Abstract
The aim of this article is to construct univariate Bernstein-type operators

(

ℬ

m

x

G

)

(

x
,
z

)

\left({{\mathcal{ {\mathcal B} }}}_{m}^{x}G)\left(x,z)

and

(

ℬ

n

z

G

)

(

x
,
z

)

,

\left({{\mathcal{ {\mathcal B} }}}_{n}^{z}G)\left(x,z),

their products

(

P

m
n

G

)

(

x
,
z

)

\left({{\mathcal{P}}}_{mn}G)\left(x,z)

,

(

Q

n
m

G

)

(

x
,
z

)

\left({{\mathcal{Q}}}_{nm}G)\left(x,z)

, and their Boolean sums

(

S

m
n

G

)

(

x
,
z

)

\left({{\mathcal{S}}}_{mn}G)\left(x,z)

,

(

T

n
m

G

)

(

x
,
z

)

\left({{\mathcal{T}}}_{nm}G)\left(x,z)

on elliptic region, which interpolate the given real valued function

G

G

defined on elliptic region on its boundary. The bound of the remainders of each approximation formula of corresponding operators are computed with the help of Peano’s theorem and modulus of continuity, and the rate of convergence for functions of Lipschitz class is computed.
CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p
Authors: Zhao Lijun , Fan Junying , Zhang Chunyang , Zhang Zhenjun , Dong Jun 
Published: March 2023

Abstract
Neuroblastoma (NB) is a common childhood cancer. Circular RNA RAN binding protein 17 (circRANBP17) has been identified to participate in diverse tumor progression. This study aims to explore the function and mechanism of circRANBP17 in NB. The levels of circRANBP17, miR-27b-3p and KDM1A in NB tissues and cells were measured by qRT-PCR. Mouse model assay was performed to investigate the effect of circRANBP17 knockdown on tumor formation in vivo. The levels of circRANBP17 and KDM1A were significantly up-regulated, and the level of miR-27b-3p was strikingly down-regulated in NB tissues and cells (SK-N-SH and SK-N-AS). Functional studies indicated that miR-27b-3p inhibitor mitigated the inhibitory effects on cell proliferation, migration, invasion and the promoting effect on cell apoptosis in SK-N-SH and SK-N-AS cells induced by circRANBP17 knockdown. Also, miR-27b-3p regulated NB cell malignancy by targeting KDM1A. Further studies revealed that miR-27b-3p inhibitor reversed the low expression of KDM1A induced by circRANBP17 knockdown. In support, circRANBP17 knockdown led to inhibition of tumor formation in vivo. In conclusion, circRANBP17 modulated KDM1A to promote cell proliferation, migration, invasion and restrain cell apoptosis in NB by sponging miR-27b-3p, and the new regulatory network may provide a theoretical basis for the further study of NB.