bluuebunny/crossref_metadata_2025_split huggingface dataset:
When Fields Are Destabilized: Mobilizing Gendered Capital to Resolve Hysteresis
Authors: Velagaleti Sunaina R , Epp Amber M 
Published: June 2023

Abstract
In the fight for legitimacy, understanding the rules of status games is critical. This idea holds true in the wedding marketplace. However, when legalization of marriage for same-sex couples disrupted the binary wedding script, the rules of the game became less clear to consumers. Couples sometimes found their gendered bodies, roles, and expressions were out of sync with the evolving script of a destabilized wedding field, thus raising legitimacy questions. This situation is defined as hysteresis—meaning delays in the realignment between habitus and the field. Hysteresis results in consumers’ disorientation about what forms of capital are valued as well as perceived absences of materials or recognition from the market. This study investigates consumers’ attempts to mobilize gendered capital to resolve hysteresis through realignment of habitus and the script. Grounded in a Bourdieuian field-level analysis of depth interviews with 30 same-sex couples and ethnographic observation in the wedding field, four consumption alignment strategies are identified that consumers leverage to address hysteresis: confronting, masking, collaborating, and experimenting. Consumers’ variations in gendered capital explain which consumption alignment strategies they use, with different possibilities for updating habitus and expanding the script. Throughout this process, consumers’ moral judgments hinder this pursuit.
GEOFABRICS LTD v FIBERWEB GEOSYNTHETICS LTD
Authors:   Patents Court
Published: June 2023

Abstract
H1 Patents — Infringement — Geocomposites — Injunctions — Permanent injunctive relief granted at trial but stayed pending appeal — Appeal unsuccessful — New product not the subject of the infringement action — Pending DNI proceedings — Whether injunction should be stayed or modified in the interim
PATIENT CHARACTERISTICS OF A REAL WORLD REGISTRY OF AMBULATORY BLOOD PRESSURE MONITORING: DATA FROM THE NEW HUNGARIAN ABPM REGISTRY
Authors: Jarai Zoltan , Nemcsik Janos , Pall Denes , Takacs Johanna , Koller Akos 
Published: June 2023

Objective:
Current European guidelines recommend ambulatory blood pressure (BP) monitoring (ABPM) for the diagnosis and follow-up of patients with known or suspected hypertension. Thus in 2021 the Hungarian Society of Hypertension initiated a nationwide registry in order to evaluate ABPM indications and patient characteristics.

Design and method:
This is an ongoing, multicenter, open-label, observational study. Data collection to an electronic case report form is performed by GPs, internists and cardiologists. For ABPM measurements Meditech-ABPM-06 monitors are used. Data of the current analysis were collected between 21.02.2021-5.10.2022. 15 835 ABPMs were performed; due to missing data or protocol deviation 1018 patients were excluded from the analysis.

Results:
10 212 patients were known and treated hypertensives (HT), and 4 571 were newly diagnosed or suspected hypertensives (nHT) (69% and 31%, respectively; females: 51.1%, males 48.9%). 76% of the nHTs were untreated. They were 12 years younger than HT patients (46.7 ± 13.9 and 59.1 ± 13.9 years old, respectively p<0.001). Besides high BP, other atherosclerotic risk factors and comorbidities were less frequent in nHT patients than in HT patients: dyslipidemia was known only in 11.8% vs. 28.7%, obesity (BMI > 30 kg/m2) in 32.2% vs. 40.3%, diabetes 2.7% vs. 13.1%, coronary heart disease in 0.8% vs. 9.6% of patients, respectively. In the HT group compared to the nHT group there were more cerebrovascular (3.3% vs 0.8%, p<0.001) and peripheral arterial diseases (1.8% vs 0.4%, p<0.001). In both groups the main indication of ABPM was high (62%) or labile (39.5%) home BP values. More ABPMs were performed in the nHT group because of the suspicion of white-coat- (19.2% vs. 9.0%, p<0.001), or masked hypertension (3.9% vs. 0.9%, p<0.001). Hypertension was diagnosed by the largest percentage in patients with suspected resistant hypertension (83%) and by the lowest percentage with suspected nocturnal hypertension (57%).

Conclusions:
The major indications for ABPM are the high or labile home BP values. Among nHTs suspected white coat hypertension is the third most important indication for ABPM, while in HT patients the follow up of therapies. Additional risk factors and comorbidities are less known in nHTs.
Age and CRP Associated With Improved Tissue Pathogen Identification in Children With Blood Culture Negative Osteomyelitis: Results From the CORTICES Multicenter Database
Authors: Burns Jessica D. , Upasani Vidyadhar V. , Bastrom Tracey P. , Baldwin Keith D. , Schoenecker Jonathan G. , Shore Benjamin J. , Brighton Brian K. , Goldstein Rachel Y. , Heyworth Benton E. , Miller Mark L. , Sanders Julia S. , Truong Walter H. ,   Childrens ORthopedic Trauma and Infection Consortium for Evidence-Based Study
Published: June 2023

Background:
Acute hematogenous osteomyelitis (AHO) is a relatively common condition in children, and identifying the offending pathogen with blood or tissue cultures aids in diagnosis and medical management while reducing treatment failure. Recent 2021 AHO clinical practice guidelines from the Pediatric Infectious Disease Society recommend obtaining routine tissue cultures, particularly in cases with negative blood cultures. The purpose of this study was to identify variables associated with positive tissue cultures when blood cultures are negative.

Methods:
Children with AHO from 18 pediatric medical centers throughout the United States through the Children’s ORthopaedic Trauma and Infection Consortium for Evidence-based Study were evaluated for predictors of positive tissue cultures when blood cultures were negative. Cutoffs of predictors were determined with associated sensitivity and specificity.

Results:
One thousand three children with AHO were included, and in 688/1003 (68.6%) patients, both blood cultures and tissue cultures were obtained. In patients with negative blood cultures (n=385), tissue was positive in 267/385 (69.4%). In multivariate analysis, age (P<0.001) and C-reactive protein (CRP) (P=0.004) were independent predictors. With age >3.1 years and CRP >4.1 mg/dL as factors, the sensitivity of obtaining a positive tissue culture when blood cultures were negative was 87.3% (80.9–92.2%) compared with 7.1% (4.4–10.9%) if neither of these factors was present. There was a lower ratio of methicillin-resistant Staphylococcus aureus in blood culture-negative patients who had a positive tissue culture 48/188 (25.5%), compared with patients who had both positive blood and tissue cultures 108/220 (49.1%).

Conclusion:
AHO patients with CRP ≤ 4.1 mg/dL and age under 3.1 years are unlikely to have clinical value from tissue biopsy that exceeds the morbidity associated with this intervention. In patients with CRP > 4.1 mg/dL and age over 3.1 years, obtaining a tissue specimen may add value; however, it is important to note that effective empiric antibiotic coverage may limit the utility of positive tissue cultures in AHO.

Level of Evidence:
Level III—Retrospective comparative study.
Pediatric Trauma and Posttraumatic Symptom Screening at Well-child Visits
Authors: DiGiovanni Stephen S. , Hoffmann Frances Rebecca J. , Brown Rebecca S. , Wilkinson Barrett T. , Coates Gillian E. , Faherty Laura J. , Craig Alexa K. , Andrews Elizabeth R. , Gabrielson Sarah M. B. 
Published: June 2023

Introduction:
Adverse childhood experiences (ACEs), including abuse or neglect, parental substance abuse, mental illness, or separation, are public health crises that require identification and response. We aimed to increase annual rates of trauma screening during well-child visits from 0% to 70%, post-traumatic stress disorder (PTSD) symptom screening for children with identified trauma from 0% to 30%, and connection to behavioral health for children with symptoms from 0% to 60%.

Methods:
Our interdisciplinary behavioral and medical health team implemented 3 plan-do-study-act cycles to improve screening and response to pediatric traumatic experiences. Automated reports and chart reviews measured progress toward goals as we changed screening methods and provider training.

Results:
During plan-do-study-act cycle 1, a chart review of patients with positive trauma screenings identified various trauma types. During cycle 2, a comparison of screening methods demonstrated that written screening identified trauma among more children than verbal screening (8.3% versus 1.7%). During cycle 3, practices completed trauma screenings at 25,287 (89.8%) well-child visits. Among screenings, 2,441 (9.7%) identified trauma. The abbreviated Post Traumatic Stress Disorder Reaction Index was conducted at 907 (37.2%) encounters and identified 520 children (57.3%) with PTSD symptoms. Among a sample of 250, 26.4% were referred to behavioral health, 43.2% were already connected, and 30.4% had no connection.

Conclusions:
It is feasible to screen and respond to trauma during well-child visits. Screening method and training implementation changes can improve screening and response to pediatric trauma and PTSD. Further work is needed to increase rates of PTSD symptomology screening and connection to behavioral health.
Eliminating Central Line Associated Bloodstream Infections in Pediatric Oncology Patients: A Quality Improvement Effort
Authors: Willis Daniel N. , Looper Karen , Malone Rema A. , Ricken Barbara , Slater Ashley , Fuller Amanda , McCaughey Meagan , Niesen Angela , Smith Joan R. , Brozanski Beverly 
Published: June 2023

Introduction:
Central Line-Associated Bloodstream Infections (CLABSI) are the largest contributor to harm across the Children’s Hospital’s Solutions for Patient Safety network. Pediatric hematology/oncology (PHO) patients are at increased risk for CLABSI due to multiple factors. Consequently, traditional CLABSI prevention strategies are insufficient to eliminate CLABSI in this high-risk population.

Methods:
Our SMART aim was to reduce the CLABSI rate by 50% from a baseline of 1.89/1000 central line days to less than 0.9/1000 central line days by December 31, 2021. We created a multidisciplinary team being mindful to identify roles and responsibilities upfront. We developed a key driver diagram and designed and implemented interventions to influence our primary outcome.

Results:
We implemented interventions and conducted Plan-Do-Study-Act cycles concurrently. We found that performing audits by directly observing tasks rather than auditing documentation resulted in more accurate compliance assessments. As a result, our CLABSI rate improved from 1.89/1000 central line days in 2020 with 11 primary CLABSI to 0.73/1000 central line days in 2021 with four primary CLABSI. Average days between events improved from 30 days in 2020 to 73 days in 2021, and we achieved an unprecedented 542 days CLABSI-free, extending into 2022.

Conclusions:
Through a multimodal approach and utilizing characteristics of high-reliability organizations, we significantly reduced primary CLABSI, approaching zero in our PHO population and doubling the average days between events. Future efforts will focus on the sustained engagement of all stakeholders and improving our safety culture.
Quality Report: Postoperative Guideline Implementation Reduces Length of Stay after Fontan Procedure
Authors: Cox Virginia , Hart Stephen , Hersey Diane , Gauntt Jennifer , Carrillo Sergio , McConnell Patrick , Simsic Janet 
Published: June 2023

Introduction:
Patients following the Fontan procedure have a physiology that results in prolonged pleural effusion, often delaying hospital discharge. The hospital length of stay (LOS) of patients following the Fontan procedure at our institution was significantly longer than the Society of Thoracic Surgery benchmark. This quality improvement project aimed to decrease hospital LOS in patients following the Fontan procedure from a baseline of 23 days to 7 days by January 1, 2021, and sustain indefinitely.

Methods:
We implemented standardized postoperative clinical practice guidelines in April 2020. We designed guidelines using previously published protocols. Key features included an ambulatory PleurX drain (BD, Franklin Lakes, N.J.), diuresis with fluid restriction, and pulmonary vasodilation with supplemental oxygen and sildenafil. All patients were discharged from the hospital with a PleurX drain in place. We compared clinical outcome variables before and after guideline implementation. As a balancing measure, we tracked 30-day readmissions.

Results:
One hundred seven patients underwent the Fontan procedure before guideline implementation from January 2015 to January 2020, with an average hospital LOS of 23 days. Postguideline implementation, 35 patients underwent the Fontan procedure from April 2020 to July 2022, with an average hospital LOS of 8 days in 2020, which further improved to an average hospital LOS of 7 days. There was no change in 30-day readmission after guideline implementation (24% pre versus 23% post; P = 0.86).

Conclusion:
Implementing clinical practice guidelines for patients following the Fontan procedure led to an over 50% reduction in hospital LOS without increasing 30-day readmission.
HYPERBILIRUBINEMIA AGGRAVATES RENAL ISCHEMIA REPERFUSION INJURY BY EXACERBATING PINK1-PARKIN–MEDIATED MITOPHAGY
Authors: Liao Pingping , Wang Xiaoyu , Dong He , Chai Dongyue , Yue Ziqi , Lyu Lin 
Published: June 2023

ABSTRACT

Background: Hyperbilirubinemia is a common perioperative complication, which is associated with acute kidney injury. Bilirubin permeabilizes mitochondrial membranes leading to mitochondrial swelling and dysfunction. In this study, we aimed to determine the association between PINK1-PARKIN–mediated mitophagy and renal ischemia-reperfusion (IR) injury aggravated by hyperbilirubinemia. Methods: A C57BL/6 mouse hyperbilirubinemia model was induced via intraperitoneal injection of bilirubin solution. In addition, a hypoxia/reoxygenation (H/R) injury model of TCMK-1 cells was established. In these models, we determined the effects of hyperbilirubinemia on oxidative stress, apoptosis, mitochondrial damage, and fibrosis. Results:
In vitro, colocalization of GFP-LC3 puncta and Mito-Tracker Red showed that the number of mitophagosomes increased in TCMK-1 cells under H/R and bilirubin condition. Silencing of PINK1 or inhibition of autophagy alleviated mitochondrial damage, oxidative stress, and apoptosis in H/R injury aggravated by bilirubin and decreased cell death detected by methyl-thiazolyl-tetrazolium. In vivo, hyperbilirubinemia increased serum creatinine level in the renal IR injury mice model. Hyperbilirubinemia enhanced apoptosis induced by renal IR. In addition, hyperbilirubinemia increased mitophagosomes and autophagosomes and disrupted mitochondrial cristae in the IR kidney. Inhibition of PINK1 or autophagy reduced histological damages by alleviating apoptosis in renal IR injury, aggravated by hyperbilirubinemia. 3-MA or PINK1-shRNA-AAV9 treatment decreased the area of collagen and proteins related to fibrosis in renal IR injury, aggravated by hyperbilirubinemia. Conclusions: We have demonstrated that hyperbilirubinemia aggravated oxidative stress, apoptosis, mitochondrial damage, and fibrosis in renal IR injury by exacerbating PINK1-PARKIN–mediated mitophagy.
ABCB1 Gene Polymorphisms Are Closely Associated With Drug-Resistant Epilepsy: Evidence Based on 377 Subjects in Chinese Pediatric Patients
Authors: Zhao Ting , Li Hong-jian , Yu Jing , Zhang Hui-lan , Feng Jie , Wang Ting-ting , Yu Lu-hai , Sun Yan 
Published: June 2023

Objective
P-glycoprotein plays a role in drug resistance of epileptic patients by limiting gastrointestinal absorption and brain access to antiseizure medications. This study aimed to evaluate the association between ABCB1 polymorphisms and drug resistance in epileptic pediatric patients.

Methods
Three hundred seventy-seven epileptic pediatric patients were treated with antiseizure medications and subsequently divided into the drug-responsive group (n = 256, 68%) and drug-resistant group (n = 121, 32%). The genomic DNA of patients in the different groups was extracted, followed by the determination of the ABCB1 gene polymorphisms using polymerase chain reaction–fluorescence staining in situ hybridization.

Results
Drug-resistant patients significantly exhibited a combined generalized and focal onset than drug-responsive patients (χ2 = 12.278, P < 0.001). The TT (χ2 = 5.776, P = 0.016) genotypes of G2677T and CT (χ2 = 6.165, P = 0.013) and TT (χ2 = 11.121, P = 0.001) genotypes of C3435T were significantly more frequent in drug-resistant patients than drug-responsive patients. Similarly, the GT-CT diplotype was significantly more frequent in drug-resistant patients than in drug-responsive patients.

Conclusion
Our study findings suggest that the ABCB1 G2677T and C3435T polymorphisms are significantly associated with drug resistance in epileptic patients.
Boosting variant-calling performance with multi-platform sequencing data using Clair3-MP
Authors: Yu Huijing , Zheng Zhenxian , Su Junhao , Lam Tak-Wah , Luo Ruibang 
Published: June 2023

AbstractBackgroundWith the continuous advances in third-generation sequencing technology and the increasing affordability of next-generation sequencing technology, sequencing data from different sequencing technology platforms is becoming more common. While numerous benchmarking studies have been conducted to compare variant-calling performance across different platforms and approaches, little attention has been paid to the potential of leveraging the strengths of different platforms to optimize overall performance, especially integrating Oxford Nanopore and Illumina sequencing data.ResultsWe investigated the impact of multi-platform data on the performance of variant calling through carefully designed experiments with a deep learning-based variant caller named Clair3-MP (Multi-Platform). Through our research, we not only demonstrated the capability of ONT-Illumina data for improved variant calling, but also identified the optimal scenarios for utilizing ONT-Illumina data. In addition, we revealed that the improvement in variant calling using ONT-Illumina data comes from an improvement in difficult genomic regions, such as the large low-complexity regions and segmental and collapse duplication regions. Moreover, Clair3-MP can incorporate reference genome stratification information to achieve a small but measurable improvement in variant calling. Clair3-MP is accessible as an open-source project at:https://github.com/HKU-BAL/Clair3-MP.ConclusionsThese insights have important implications for researchers and practitioners alike, providing valuable guidance for improving the reliability and efficiency of genomic analysis in diverse applications.
Full-field strain distribution in hierarchical electrospun nanofibrous Poly-L(lactic) acid and Collagen based scaffolds for tendon and ligament tissue regeneration: a multiscale study
Authors: Sensini Alberto , Stamati Olga , Marchiori Gregorio , Sancisi Nicola , Gotti Carlo , Giavaresi Gianluca , Cristofolini Luca , Focarete Maria Letizia , Zucchelli Andrea , Tozzi Gianluca 
Published: June 2023

AbstractRegeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly (L-lactic) acid and collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal tensile and compressive strains detected the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.Graphical Abstract
A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference
Authors: Cheng An-Po , Lederer Bernhard , Oberkofler Lorenz , Huang Lihong , Platten Fabian , Dunker Florian , Tisserant Constance , Weiberg Arne 
Published: June 2023

AbstractSmall RNAs act as fungal pathogen effectors that silence host target genes to promote infection, a virulence mechanism termed cross-kingdom RNA interference (RNAi). The essential pathogen factors of cross-kingdom small RNA production are largely unknown. We here characterized the RNA-dependent RNA polymerase (RDR)1 in the fungal plant pathogenBotrytis cinereathat is required for pathogenicity and cross-kingdom RNAi.B. cinerea bcrdr1knockout (ko) mutants exhibited reduced pathogenicity and loss of cross-kingdom small RNAs. We developed a novel “switch-on” GFP reporter to study cross-kingdom RNAi in real-time within the living plant tissue which highlighted thatbcrdr1ko mutants were compromised in cross-kingdom RNAi. Moreover, blocking seven pathogen cross-kingdom small RNAs by expressing a short-tandem target mimic RNA in transgenicArabidopsis thalianaled to reduced infection levels of the fungal pathogenB. cinereaand the oomycete pathogenHyaloperonospora arabidopsidis. These results demonstrate that cross-kingdom RNAi is significant to promote host infection and making pathogen small RNAs an effective target for crop protection.
Haplotype-resolve genome assembly and resequencing provide insights into the origin and domestication of modern rose
Authors: Zhang Zhao , Liu Yang , Yang Tuo , Wu Shan , Sun Honghe , Wu Jie , Li Yonghong , Zheng Yi , Ren Haoran , Yang Yuyong , Shi Shaochuan , Wang Wenyan , Pan Qi , Lian Lijuan , Duan Shaowen , Zhu Yingxiong , Cai Youming , Zhou Hougao , Zhang Hao , Tang Kaixue , Cui Jiaopeng , Gao Dan , Chen Liyang , Jiang Yunhe , Sun Xiaoming , Zhou Xiaofeng , Fei Zhangjun , Ma Nan , Gao Junping 
Published: June 2023

AbstractModern rose (Rosa hybrida) is a recently formed interspecific hybrid and has become one of the most important and widely cultivated ornamentals. Here, we report the haplotype-resolved chromosome-scale genome assembly of the tetraploidR. hybrida‘Samantha’ and a genome variation map of 233Rosaaccessions involving various wild species, and old and modern cultivars. The two subgenomes of ‘Samantha’ show no significant global bias in gene loss but substantial expression bias, and homoeologous exchanges are frequently observed between subgenomes. Population genomic and genomic composition analyses reveal the contributions of wildRosaspecies to modern roses, and highlight thatR. odorataand its derived cultivars contribute more to modern roses thanR. chinensis‘Old Blush’. Furthermore, selective sweeps during modern rose breeding associated with major agronomic traits, including continuous flowering, floral organ identity, flower senescence, and disease resistance, are identified. This study provides insights into the genetic basis of modern rose origin and breeding history, and offers unprecedented genomic resources for rose improvement.