bluuebunny/crossref_metadata_2025_split huggingface dataset:
A subset of highly responsive transcription factors upon tomato infection by pepino mosaic virus
Authors: Tsitsekian D. , Daras G. , Templalexis D. , Avgeri F. , Lotos L. , Orfanidou C. G. , Ntoukakis V. , Maliogka V. I. , Rigas S. 
Published: March 2023

Abstract

Plants have evolved well‐tuned surveillance systems, including complex defence mechanisms, to constrain pathogens. TFs are master regulators of host molecular responses against plant pathogens. While PepMV constitutes a major threat to the global tomato production, there is still a lack of information on the key TFs that regulate host responses to this virus.

A combinatorial research approach was applied relying on tomato transcriptome analysis, RT‐qPCR validation, phylogenetic classification, comparative analysis of structural features, cis‐regulatory element mining and in silico co‐expression analysis to identify a set of 11 highly responsive TFs involved in the regulation of host responses to PepMV.

An endemic PepMV isolate, generating typical mosaic symptoms, modified expression of ca. 3.3% of tomato genes, resulting in 1,120 DEGs. Functional classification of 502 upregulated DEGs revealed that photosynthesis, carbon fixation and gene silencing were widely affected, whereas 618 downregulated genes had an impact mainly on plant defence and carotenoid biosynthesis. Strikingly, all 11 highly responsive TFs carried abiotic stress response cis‐regulatory elements, whereas five of them were better aligned with rice than with Arabidopsis gene homologues, suggesting that plant responses against viruses may predate divergence into monocots and dicots. Interestingly, tomato C2H2 family TFs, ZAT1‐like and ZF2, may have distinct roles in plant defence due to opposite response patterns, similar to their Arabidopsis ZAT10 and ZAT12 homologues.

These highly responsive TFs provide a basis to study in‐depth molecular responses of the tomato–PepMV pathosystem, providing a perspective to better comprehend viral infections.
Association of revision of Money Lending Business Act and suicide rate in Japan: An interrupted time series analysis
Authors: Fukuya Yoshifumi , Nawa Nobutoshi , Morita Ayako , Fujiwara Takeo 
Published: March 2023

AbstractObjectiveDebt problems are associated with suicide. In Japan, the Money Lending Business (MLB) Act was revised in December 2006 and fully enforced in June 2010 to reduce multiple debt problems. This study aimed to examine the association between the full enforcement of the revised act and suicide rates.MethodsAn interrupted time series analysis was used to the national registry data of death records between January 2000 and December 2016. Stratified analysis was conducted to assess the differences between gender and age groups.ResultsThe effects of the full enforcement on the suicide rate were the greatest among men aged 40–64 years; after the enforcement, there was a significant decrease of −0.3085 per 100,000 persons in the level of the suicide rate (95% confidence interval [CI] −0.529 to −0.0881) and −0.0165 per 100,000 persons (95% CI −0.0215 to −0.0115) in the trend. Among women aged 40–64 and ≥65 years, an increase in the level of the suicide rate was observed after the enforcement.ConclusionsThe full enforcement of the revised MLB Act was associated with decreased suicide rates, particularly among middle‐aged men. Policy interventions for addressing debt problems may have the potential to prevent suicide.
Iris angiography in ADAMTS10 mutant dogs with open‐angle glaucoma (ADAMTS10‐OAG)
Authors: Pytak Richard A. , Pirie Christopher G. , Harman Christine D. , Anderson Amanda L. , Yao Shay Xuejing , Komáromy András M. 
Published: March 2023

AbstractObjectiveTo evaluate anterior segment angiographic findings in hypertensive ADAMTS10‐open‐angle glaucoma (ADAMTS10‐OAG) eyes as compared to normotensive control eyes.Animals StudiedNine ADAMTS10‐OAG beagles and four wild‐type control dogs.ProceduresAnterior segment angiography was performed under general anesthesia following intravenous injection of indocyanine green (ICG; 1 mg/kg) and sodium fluorescein (SF; 20 mg/kg) using a Heidelberg Spectralis® confocal scanning laser ophthalmoscope. Time to onset of iridal angiographic phases and the presence/severity of dye leakage into the iris stromal and/or aqueous humor were recorded. Group findings were compared, and multiple linear regression analysis was performed to identify potential factor associations with disease status.ResultsTime to onset of all angiographic phases visualized using ICG was significantly prolonged while time to onset of SF leakage into the aqueous humor was significantly reduced in glaucomatous eyes compared to controls. Only glaucomatous eyes (n = 9) demonstrated evidence of SF stromal leakage. Mean intraocular pressure (IOP) and age were significantly higher, while mean cardiac pulse was significantly lower in glaucomatous eyes compared to controls. Blood pressure and ocular perfusion pressure were not significantly different between groups. Multiple linear regression analysis, controlling for age, IOP, and pulse demonstrated glaucoma, was not predictive of the time to onset of any angiographic phase, stromal, or aqueous humor leakage. However, pulse was a significant factor contributing to the severity of aqueous humor leakage.ConclusionsA compromised vascular supply to the anterior segment exists in dogs with ADAMTS10‐OAG. These observations warrant further exploration of what role altered perfusion and/or disruption to the blood–aqueous barrier may play.
The effect of 4 weeks of high‐intensity interval training and 2 weeks of detraining on cardiovascular disease risk factors in male adolescents
Authors: Kranen Sascha H. , Oliveira Ricardo S. , Bond Bert , Williams Craig A. , Barker Alan R. 
Published: March 2023

AbstractHigh‐intensity interval training (HIIT) represents an effective method to improve cardiometabolic health in adolescents. This study aimed to investigate the effect of 4 weeks of HIIT followed by 2 weeks of detraining on vascular function and traditional cardiovascular disease (CVD) risk factors in adolescent boys. Nineteen male adolescents (13.3 ± 0.5 years) were randomly allocated to either a training (TRAIN, n = 10) or control (CON, n = 9) group. Participants in TRAIN completed 4 weeks of HIIT running with three sessions per week. Macro‐ (flow‐mediated dilatation, FMD) and microvascular (peak reactive hyperaemia, PRH) function, body composition (fat mass, fat free mass, body fat percentage) and blood biomarkers (glucose, insulin, total cholesterol, high‐ and low‐density lipoprotein, triacylglycerol) were assessed pre‐, 48 h post‐ and 2 weeks post‐training for TRAIN and at equivalent time points for CON. Following training, FMD was significantly greater in TRAIN compared to CON (9.88 ± 2.40% and 8.64 ± 2.70%, respectively; P = 0.036) but this difference was lost 2 weeks after training cessation (8.22 ± 2.47% and 8.61 ± 1.99%, respectively; P = 0.062). No differences were detected between groups for PRH (P = 0.821), body composition (all P > 0.14) or blood biomarkers (all P > 0.18). In conclusion, 4 weeks of HIIT improved macrovascular function; however, this training period did not measurably change microvascular function, body composition or blood biomarkers. The reversal of the FMD improvement 2 weeks post‐training highlights the importance of the continuation of regular exercise for the primary prevention of CVD.
Research on Productivity Evaluation Method Based on Shale Gas Well Early Flow Stage Data
Authors: Huang Yize , Li Xizhe , Guo Wei , Liu Xiaohua , Lin Wei , Qian Chao , Zhou Mengfei , Cui Yue , Shi Xiaomin 
Published: March 2023

Since the accurate early gas well production regime is related to the production period and final productivity, it is crucial to make better use of early flow stage production data for gas well productivity evaluation. Absolute open flow potential (AOF) and estimated ultimate recovery (EUR) are essential parameters for evaluating the productivity of shale gas wells. This study establishes new AOF calculation methods for the early production stage. The analytical model can calculate the AOF only by using stable pressure and production data in the flowback stage, which greatly improves the efficiency of productivity evaluation. Three methods have, respectively, calculated the productivity of the shale gas wells above 3500 m in the Luzhou block. The results show that well L2-3 has the highest AOF, averaging

278.1
×

10

4

m

3

/
d

, whereas well Y2-8 has the lowest AOF, averaging

100.2
×

10

4

m

3
/

d

. Different AOF calculation methods are identified for gas wells in different stages of production. For gas wells in the initial unstable flow stage, a pseudogas production index method is recommended. A water production index analysis method, with lower evaluation results, is proposed for gas wells in the flowback stage. A modern production decline analysis method is found to be preferred for calculating the EUR of deep shale gas wells. Well L2-3 has the highest average EUR of

1.26
×

10

8

m

3

, whereas well Y2-8 has the lowest average EUR of

0.42
×

10

8

m

3

. The Blasingame method is recommended for medium-to-high-production gas wells, whereas a normalized pressure integral method is suggested for low-production wells. A strong exponential quantitative link between the AOF and the EUR shows that a fracture system’s initial productivity has a significant impact on a well’s EUR. The findings of this study enrich the productivity evaluation system, increase the accuracy of productivity evaluation results, and provide theoretical support for deep shale gas wells.
Reprogramming Cancer into Antigen-Presenting Cells as a Novel Immunotherapy
Authors: Linde Miles H. , Fan Amy C. , Köhnke Thomas , Trotman-Grant Aaron C. , Gurev Sarah F. , Phan Paul , Zhao Feifei , Haddock Naomi L. , Nuno Kevin A. , Gars Eric J. , Stafford Melissa , Marshall Payton L. , Dove Christopher G. , Linde Ian L. , Landberg Niklas , Miller Lindsay P. , Majzner Robbie G. , Zhang Tian Yi , Majeti Ravindra 
Published: March 2023

Abstract

Therapeutic cancer vaccination seeks to elicit activation of tumor-reactive T cells capable of recognizing tumor-associated antigens (TAA) and eradicating malignant cells. Here, we present a cancer vaccination approach utilizing myeloid-lineage reprogramming to directly convert cancer cells into tumor-reprogrammed antigen-presenting cells (TR-APC). Using syngeneic murine leukemia models, we demonstrate that TR-APCs acquire both myeloid phenotype and function, process and present endogenous TAAs, and potently stimulate TAA-specific CD4+ and CD8+ T cells. In vivo TR-APC induction elicits clonal expansion of cancer-specific T cells, establishes cancer-specific immune memory, and ultimately promotes leukemia eradication. We further show that both hematologic cancers and solid tumors, including sarcomas and carcinomas, are amenable to myeloid-lineage reprogramming into TR-APCs. Finally, we demonstrate the clinical applicability of this approach by generating TR-APCs from primary clinical specimens and stimulating autologous patient-derived T cells. Thus, TR-APCs represent a cancer vaccination therapeutic strategy with broad implications for clinical immuno-oncology.

Significance:
Despite recent advances, the clinical benefit provided by cancer vaccination remains limited. We present a cancer vaccination approach leveraging myeloid-lineage reprogramming of cancer cells into APCs, which subsequently activate anticancer immunity through presentation of self-derived cancer antigens. Both hematologic and solid malignancies derive significant therapeutic benefit from reprogramming-based immunotherapy.
This article is highlighted in the In This Issue feature, p. 1027
A Novel Ubiquitin Complex Regulates Aneuploid Epithelial Tumors by Moderating an Integrated Stress Response
Authors: Leli Nektaria Maria , Koumenis Constantinos 
Published: March 2023

Summary:Tumor fitness coessentiality gene analysis that aims to expand the repertoire of druggable targets reveals a novel ubiquitin ligase complex, the BICR6 module. Along with the other complex members (UBA6, KCMF1, and UBR4), BIRC6 selectively contributes to the survival of a subset of epithelial tumors with a high degree of aneuploidy by ubiquitinating and suppressing HRI, a component of the integrated stress response adaptive pathway.See related article by Cervia et al., p. 766 (2).
Characterization of the Morning Transition over the Gentle Slope of a Semi-Isolated Massif
Authors: Farina Sofia , Marchio Mattia , Barbano Francesco , di Sabatino Silvana , Zardi Dino 
Published: March 2023

Abstract
This paper investigates the surface-layer processes associated with the morning transition from nighttime downslope winds to daytime upslope winds over a semi-isolated massif. It provides an insight into the characteristics of the transition and its connection with the processes controlling the erosion of the temperature inversion at the foot of the slope. First, a criterion for the identification of days prone to the development of purely thermally driven slope winds is proposed and adopted to select five representative case studies. Then, the mechanisms leading to different patterns of erosion of the nocturnal temperature inversion at the foot of the slope are analyzed. Three main patterns of erosion are identified: the first is connected to the growth of the convective boundary layer at the surface, the second is connected to the descent of the inversion top, and the third is a combination of the previous two. The first pattern is linked to the initiation of the morning transition through surface heating, and the second pattern is connected to the top-down dilution mechanism and so to mixing with the above air. The discriminating factor in the determination of the erosion pattern is identified in the partitioning of turbulent sensible heat flux at the surface.

Significance Statement
The purpose of this study is to improve our understanding of the thermally driven slope circulations with a focus on the unsteady processes associated with the morning transition and the erosion patterns of the nocturnal temperature inversion, so far in the literature less investigated and understood than the evening transition. Understanding this diurnal process will advance our abilities to model it and to improve the accuracy of weather forecasting in complex terrain.
Perioperative renal function change after transcatheter aortic valve replacement: A single-center retrospective study in China
Authors: Dong Zhe , Yao Jing , Li Rui , Liu Xinmin , Yuan Fei , Luo Taiyang , Zhang Qian , Song Guangyuan 
Published: March 2023

Background In recent years, the kidney function after Transcatheter Aortic Valve Replacement (TAVR) has gradually become a hot spot that arouse extensive attention.Our study is aimed to evaluate the incidence and predictors of acute kidney recovery (AKR) after TAVR. Methods A total of 102 patients undergoing TAVR in Beijing Anzhen Hospital from June 2021 to March 2022 were enrolled in our study. Patients were divided into AKR group ( n = 54), unchanged group ( n = 40) and acute kidney injury (AKI) group ( n = 8) based on the percent change of estimated glomerular filtration rate (eGFR). Univariate analysis was used to compare the differences in general clinical characteristics and other related indicators between the three groups to analyze the risk factors of AKR. Results The incidence of AKR was 53% (54/102) after TAVR. Multivariate analysis showed that the incidence of age and proportion of severe NYHA class (III or IV) was significantly higher in the AKR group while renal dysfunction (eGFR <60 mL/min/1.73 m2) was lower. Besides, fluid management/volume therapy was significantly different among the three groups. Conclusions AKR is a generalizable phenomenon occurring frequently after TAVR. The age, proportion of severe NYHA class and the baseline renal function are independent predictors of AKR events in patients with severe aortic stenosis undergoing TAVR.
Evaluating the efficacy of CGRP mAbs and gepants for the preventive treatment of migraine: A systematic review and network meta-analysis of phase 3 randomised controlled trials
Authors: Haghdoost Faraidoon , Puledda Francesca , Garcia-Azorin David , Huessler Eva-Maria , Messina Roberta , Pozo-Rosich Patricia 
Published: March 2023

Background Several novel treatments targeting the calcitonin gene-related peptide pathway have been developed for migraine. We evaluated the efficacy of these medications, including atogepant, rimegepant, erenumab, eptinezumab, fremanezumab, and galcanezumab, for the prevention of migraine via network meta-analysis. Methods Databases, including MEDLINE via PubMed, EMBASE, and Cochrane central, were systematically reviewed, and all eligible phase 3 randomised controlled trials were included. Results Nineteen studies (n = 14,584 participants) were included. Studies included episodic (n = 11) and chronic (n = 4) migraine or both (n = 4). All interventions, except for eptinzumab 30 mg, significantly reduced mean monthly migraine days compared to placebo. All medications had a higher ≥50% responder rate than placebo and results were statistically significant in those with the subcutaneous or intravenous route of administrations, but not with the oral one. All medications significantly reduced mean monthly headache days, although no data for this outcome was available for rimegepant, and mean monthly acute medication days, with no data for eptinezumab. Conclusion The results show that medications targeting calcitonin gene-related peptide were effective in preventing migraine compared to placebo. Considering limitations of single studies, different populations such as episodic and chronic migraine, and the absence of head-to-head trials, all novel treatments decreased mean monthly migraine and headache days, and showed higher 50%, 75% and 100% responder rates than placebo. Trial registration: PROSPERO registration: CRD42022310579
Patient perspectives on peritoneal dialysis (PD) and the PD catheter: Strategies and Solutions
Authors: Fissell Rachel B , Wysocki Matthew , Bonnet Kemberlee , Abifaraj Farah , Cavanaugh Kerri L , Nair Devika , Umeukeje Ebele M , Wild Marcus G , Liddell Peter , Spangler Matthew , Schlundt David 
Published: March 2023

Introduction: Peritoneal dialysis (PD) catheter complications reduce quality of life and increase risks for hospitalizations, for unplanned transitions to haemodialysis and for death. Patient PD catheter management is crucial for safe, sustained PD. Patient perspectives on strategies for living with PD and using a PD catheter may inform efforts to reduce PD catheter complications, increase individual patient PD modality persistence, and thus increase overall home dialysis prevalence. Methods: We interviewed 32 adult PD patients in Nashville, Tennessee. Qualitative analyses included (1) isolation of themes, (2) development of a coding system and (3) creation of a conceptual framework using an inductive–deductive approach. Results: Challenges identified by patients as important included drain pain, difficulty eating and sleeping, and fear of peritonitis. Coping strategies included repositioning while draining, adjusting eating patterns, and development of PD patient and helper knowledge and confidence, especially at home after initial training. Patients described a trial-and-error iterative process of trying multiple strategies with input from multiple sources, which led to individualised solutions. Conclusions: The trial-and-error process may be crucial for maintaining PD. Individual patient success with PD may be promoted by creating expectations during training that a solution may require multiple attempts, and by a reimbursement policy that supports robust nursing support for safe progression through the trial-and-error process, particularly in the first few months for incident patients. Interventions to support patient motivation and optimal coping behaviour may also support an increase in PD modality duration for individual patients, and thus increase overall PD prevalence.
Successful treatment of non-Langerhans cell histiocytosis with the MEK inhibitor trametinib: a multicenter analysis
Authors: Aaroe Ashley , Kurzrock Razelle , Goyal Gaurav , Goodman Aaron M. , Patel Harsh , Ruan Gordon , Ulaner Gary , Young Jason , Li Ziyi , Dustin Derek , Go Ronald S. , Diamond Eli L. , Janku Filip 
Published: March 2023

Abstract
Erdheim-Chester disease (ECD) and Rosai-Dorfman disease (RDD) are rare non-Langerhans cell histiocytoses (non-LCHs), for which therapeutic options are limited. MAPK pathway activation through BRAFV600E mutation or other genomic alterations is a histiocytosis hallmark and correlates with a favorable response to BRAF inhibitors and the MEK inhibitor cobimetinib. However, there has been no systematic evaluation of alternative MEK inhibitors. To assess the efficacy and safety of the MEK inhibitor trametinib, we retrospectively analyzed the outcomes of 26 adult patients (17 with ECD, 5 with ECD/RDD, 3 with RDD, and 1 with ECD/LCH) treated with orally administered trametinib at 4 major US care centers. The most common treatment-related toxicity was rash (27% of patients). In most patients, the disease was effectively managed at low doses (0.5-1.0 mg trametinib daily). The response rate of the 17 evaluable patients was 71% (73% [8/11] without a detectable BRAFV600E achieving response). At a median follow-up of 23 months, treatment effects were durable, with a median time-to-treatment failure of 37 months, whereas the median progression-free and overall survival were not reached (at 3 years, 90.1% of patients were alive). Most patients harbored mutations in BRAF (either classic BRAFV600E or other BRAF alterations) or alterations in other genes involved in the MAPK pathway, eg, MAP2K, NF1, GNAS, or RAS. Most patients required lower than standard doses of trametinib but were responsive to lower doses. Our data suggest that the MEK inhibitor trametinib is an effective treatment for ECD and RDD, including those without the BRAFV600E mutation.