bluuebunny/crossref_metadata_2025_split huggingface dataset:
GAMBARAN SPASIAL HUBUNGAN ANTARA FAKTOR LINGKUNGAN DAN EKONOMI DENGAN STUNTING BALITA DI PROVINSI NUSA TENGGARA TIMUR
Authors: Nashriyah Siti Fadhilatun , Makhful Martya Rahmaniati , Devi Yuli Puspita 
Published: May 2023

Background: The President through Presidential Regulation Number 72 of 2021 is committed to accelerating the reduction of stunting in Indonesia. The stunting priority areas also continue to be increased from year to year. In Indonesia, East Nusa Tenggara Province is the province with the highest prevalence of stunting, namely 37.8% in 2021.
Objective: To find out the spatial picture and the correlation between environmental factors and economic factors with the prevalence of stunting in East Nusa Tenggara Province in 2021.
Methods: The research design is an ecological study with a spatial approach. This study uses secondary data in the form of reports issued by the Indonesian Ministry of Health (SSGI: Indonesian Nutritional Status Study) and the BKKBN (PK: Family Data Collection) in 2021 with district/city-level analysis units in East Nusa Tenggara Province. Data analysis used a correlation test and mapping was carried out using the QGIS 2.8.1 application.
Results: The prevalence of stunting in NTT Province tends to be high in the eastern part while the risk factors for stunting (unfavorable environment and economy) tend to be high in the western part. The correlation test showed that the correlation between the prevalence of stunting and the poor was 0.165 (p = 0.463); with inadequate latrines of 0.420 (p = 0.052); and with inadequate drinking water sources of 0.426 (p = 0.048).
NG2-positive pericytes regulate homeostatic maintenance of slow-type skeletal muscle with rapid myonuclear turnover
Authors: Tatsukawa Takamitsu , Kano Kohei , Nakajima Kei-ichi , Yazawa Takashi , Eguchi Ryoji , Kabara Maki , Horiuchi Kiwamu , Hayasaka Taiki , Matsuo Risa , Hasebe Naoyuki , Azuma Nobuyoshi , Kawabe Jun-ichi 
Published: May 2023

Abstract
Background
Skeletal muscle comprises almost 40% of the human body and is essential for movement, structural support and metabolic homeostasis. Size of multinuclear skeletal muscle is stably maintained under steady conditions with the sporadic fusion of newly produced myocytes to compensate for the muscular turnover caused by daily wear and tear. It is becoming clear that microvascular pericytes (PCs) exhibit myogenic activity. However, whether PCs act as myogenic stem cells for the homeostatic maintenance of skeletal muscles during adulthood remains uncertain.
Methods
We utilized PC-fused myofibers using PC-specific lineage tracing mouse (NG2-CreERT/Rosa-tdTomato) to observe whether muscle resident PCs have myogenic potential during daily life. Genetic PC deletion mouse model (NG2-CreERT/DTA) were used to test whether PC differentiate to myofibers for maintenance of muscle structure and function under homeostatic condition.
Results
Under steady breeding conditions, tdTomato-expressing PCs were infused into myofibers, and subsequently, PC-derived nuclei were incorporated into myofibers. Especially in type-I slow-type myofibers such as the soleus, tdTomato+ myofibers were already observed three days after PC labeling; their ratio reached a peak (approximately 80%) within one month and was maintained for more than one year. Consistently, the NG2+ PC-specific deletion induced muscular atrophy in a slow-type myofiber-specific manner under steady breeding conditions. The number of myonucleus per volume of each myofiber was constant during observation period.
Conclusions
These findings demonstrate that the turnover of myonuclei in slow-type myofibers is relatively fast, with PCs acting as myogenic stem cells—the suppliers of new myonuclei under steady conditions—and play a vital role in the homeostatic maintenance of slow-type muscles.
Spatiotemporal variability of extreme precipitation in East of northwest China and associated large-scale circulation factors
Authors: Guo Yuhong , Yan Xiaodong , Gao Zhibo , Song Shuaifeng 
Published: May 2023

Abstract
Spatial and temporal distributions and influencing factors of extreme precipitation are important bases for coping with future climate change. The spatiotemporal variability and affecting factors of extreme precipitation indices (EPIs) in East of northwest China (ENW) during 1961–2015 were investigated using a series of approaches such as modified Mann-Kendall trend test, Hurst exponent, Ensemble empirical mode decomposition (EEMD), and geodetector model. The results showed that CDD and CWD decreased significantly (P < 0.01), with rates of 1.4 days/decade and 0.07 days/decade, respectively. EPIs in ENW exhibited an obvious heterogeneity. CDD gradually increased from the southeast to the northwest. The remaining EPIs generally showed the opposite trend. Some stations in ENW may experience more extreme precipitation events in the future. Geodetector results demonstrated that large-scale circulation factors had a significant impact on EPIs in ENW. The influence of large-scale climate factors on EPIs was concentrated in nonlinear enhancement, and Nino3.4 and SO were the dominant driving factors that played a major role in the variability of EPIs. The results of this study provided a reference for ENW and other arid and semi-arid regions to cope with extreme climates and develop corresponding strategies.
A strain reconstruction method for uncertain boundary conditions
Authors: Huo junzhou , Ge Lihan , Zhang zhange , Wang zejiang , Zhao Xin 
Published: May 2023

Abstract
Affected by complex boundary conditions and redundant structural assembly, it is difficult to collect stress and strain information on key structures of complex mechanical equipment, resulting in difficult life monitoring and large calculation errors. The purpose of this study is to provide reliable strain data for accurate life prediction and solve the problem of obtaining critical structural strain under uncertain loads and other complex boundary conditions. In this paper, a neural network training dataset is established based on finite element analysis data and experimental data, and the neural network architecture and parameter optimization are carried out for each data set. Finally, the strain reconstruction model based on a neural network is established, which solves the two problems of accurate equivalence from structure to strain and equivalence from strain to load. Feature experimental samples were designed to verify the universality of the research method. The model is experimentally verified with an average error of less than 5%. It realizes the global strain reconstruction from the local measurement point to the overall structure, which can be used for real-time and full-cycle life monitoring of the structure;
SHP099 Combination Inhibits the Malignant Biological Behavior of L-OHP/5-fu Resistant Colorectal Cancer Cells by Regulating Energy Metabolism Reprogramming
Authors: Wang Meilian , Yu Kun , Fu Wen , Yang Lihong 
Published: May 2023

Abstract

Methods
HT29 and SW480 cell lines were fostered in media containing L-OHP or 5-Fu to establish drug-resistant strains. Injected subcutaneously HT29 and SW480 drug-resistant cells into the ventral of nude mice at a dose of 5 × 106 to establish CRC drug-resistant animal models. CCK-8, Western blot, flow cytometry, Transwell and kit detection were used to detect the regulation mechanism of energy metabolism reprogramming in drug-resistant CRC cells. Results: Compared with non-resistant strains, L-OHP/5-fu resistant strains had stronger ability of metabolic reprogramming. Functionally, SHP099 can restrain the metabolic reprogramming of L-OHP/5-fu resistant strains, and then restrain the cell proliferation, cloning, migration and tumor spheroid formation of L-OHP/5-fu resistant strains. Downstream mechanism studies have shown that SHP099 interferes with the metabolic reprogramming of L-OHP/5-fu drug-resistant strains by suppressing PI3K/AKT pathway, thereby restraining the malignant biological behavior of L-OHP/5-fu drug-resistant strains and alleviating CRC. Conclusion: The combination of SHP099 can restrain the malignant biological behavior of L-OHP/5-fu resistant CRC cells and alleviate the progression of CRC by interfering with the reprogramming of energy metabolism.
Efficacy of RESOLVE DWI in the staging of Graves ’ophthalmopathy compared with conventional MRI
Authors: Yang Libin , Yu Shun , Chen Meihui , Qiu Huiling 
Published: May 2023

Abstract
Purpose. To determine the efficacy of RESOLVE DWI in the staging of GO compared with conventional magnetic resonance imaging (MRI).
Methods. A total of 58 consecutive GO patients were enrolled and divided into two groups according to the patients’ clinical activity score (CAS). Signal intensity ratio (SIR) of extraocular muscle (EOM) on coronal T2-weighted imaging (T2WI) with fat suppression and apparent diffusion coefficient (ADC) value of EOM for ADC map was measured and analyzed.
Results. Both SIR and ADC values in the active GO were significantly higher than those of inactive GO(P＜0.001). SIR and ADC values were positively correlated with CAS (r＝ 0.58, 0.55; P<0.001).When SIR of 2.2 and ADC value of 1467.64 × 10－6 mm2/s of the EOM were used as threshold values for staging GO, respectively, the best results were obtained with a sensitivity of 64%,59%, and specificity of 88%,86%. There was no significant difference in area under the curve between SIR and ADC.
Conclusion. RESOLVE DWI has the potential to be a valuable MRI tool in the staging of GO in addition to conventional MRI.
Evaluation of an adapted semi-automated DNA extraction for human salivary shotgun metagenomics
Authors: Almeida Mathieu , Meslier Victoria , Menozzi Elisa , David Aymeric , Morabito Christian , Pozo Sara Lucas Del , Famechon Alexandre , North Janet , Quinquis Benoit , Koletsi Sofia , Macnaughtan Jane , Mezabrovschi Roxana , Ehrlich S. Dusko , Schapira Anthony HV. 
Published: May 2023

Abstract
The importance of oral microbiota has been recently highlighted in human health, such as in Parkinson disease, notably using shotgun metagenomics. One key aspect for efficient shotgun metagenomic analysis relies on optimal microbial sampling and DNA extraction, generally implementing commercial solutions developed to improve sample collection and preservation and provide high DNA quality and quantity for downstream analysis. Because metagenomic studies are today performed on a large number of samples, the next evolution to increase study throughput is with DNA extraction automation. In this study, we proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer. While similar DNA yields were observed between the protocols, our semi-automated DNA protocol significantly generated higher DNA fragment sizes. Moreover, we showed that the oral microbiome composition was equivalent between DNA extraction methods, even at the species level. This study demonstrates that our semi-automated protocol is suitable for shotgun metagenomic analysis, while allowing for a better sample treatment logistic with reduced technical variability and without compromising the structure of the oral microbiome.
Concordance between Immunohistochemistry and MSI analysis for detection of MMR/MSI status in colorectal cancer patients
Authors: Faizee Muhammad Ishaque , Talib Norlelawati A. , Hamdan Asmah Hanim Bt. , Abdullah Nor zamzila Bt. , Rahimi Bilah Ahmad , Haidary Ahmed Maseh , Hanifi Ahmed Nasir 
Published: May 2023

Abstract
Background
Recently, screening of colorectal cancer (CRC) patients for mismatch repair/microsatellite instability (MMR/MSI) status is widely practiced due to its potential predictive and prognostic roles and a screening tool to reveal Lynch Syndrome (LS). The purpose of the study was to evaluate concordance between immunohistochemistry (IHC) and MSI analysis methods for detection of MMR/MSI status in colorectal cancer patients in Kuantan, Pahang.
Methods
Fifty selected CRC cases of deficient mismatch repair (dMMR) and proficient mismatch repair (pMMR) which were identified immunohistochemically in the previous study were subjected to MSI analysis. MSI Analysis System 1.2 (Promega) was utilized.
Results
The results of MSI analysis method showed MSI-High: 26% (13/50), MSI-Low: 6% (3/50), and Microsatellite Stable: 68% (34/50). The concordance was perfect (0.896, Kappa value) between MSI analysis and IHC methods for the assessment of MMR/MSI status in CRC patients. The discordance was only 4% (2/50). MSI analysis identified all dMMR cases determined by IHC except one case. The obtained frequency of dMMR and pMMR patients was 11.4% (14/123) and 88.6% (109/123) by IHC method, respectively.
Conclusion
Our findings support the universal practice of evaluating the MMR/MSI status in all newly diagnosed CRC patients. Based on the perfect concordance of two methods, the method of choice is based on the availability of expertise and equipments. IHC is highly appreciable method due to its feasibility and reproducibility.
MWCNTs Dispersion adopting GA and Its Application towards Copper Tailings-Based Cementitious Materials
Authors: Xiang Bingzhi , Cheng Ruifeng , Zhu Jielu , Zhou Yong , Peng Xiaoying , Song Junwei , Wu Junhong 
Published: May 2023

Abstract
Hydrophobic carbon nanotubes are hardly to disperse in water and prone to agglomerate when poured with Copper Tailing-Based Cementitious Material (CTCM). Multi-walled carbon nanotubes (MWCNTs) + Arabic Gum (GA) dispersions were prepared by a novel method of synergistic optimization of concentration, controlling low-frequency ultrasonic time and setting the ambient temperature with non-toxic anionic surfactant GA as surfactant. The results of UV-Vis spectroscopy showed that the high stability MWCNTs + GA dispersion with low aggregation area (<1.2%) and low aggregation beam size (<219 nm) have been prepared by using 1.7mmol / l GA. The effects of highly stable MWCNTs dispersion on the mechanical properties, microstructure and durability of CTCM were studied. The results of SEM, XRD and EDX showed that GA significantly enhanced the dispersion of MWCNT in aqueous solution at a suitable concentration (mass ratio of GA:CNTs =1:1). The microstructure of the prepared CTCM by high stability MWCNTs dispersion was optimized obviously, and the mechanical properties and durability were improved significantly. This method solves the dual problem of MWCNTs not being fully dispersed in aqueous solution and being easily re-agglomerated in cementitious materials, as well as finding a breakthrough for the low cost and industrialization of tailings cement-based composite cementitious materials.
Phylogenetic and morphological evidence reveals six new species of boletus from southern China
Authors: Zhou Fan , Gao Yang , Song Hai-Yan , Hu Hai-Jing , Yang Wen-Juan , Zhang Wei , Liao Li-Yu , Fang Yi , Cheng Lin , Hu Dian-Ming 
Published: May 2023

Abstract
Fungi of the order Boletales are extremely important in both ecology and economy, since most of them are ectomycorrhizal fungi, which play vital roles in maintaining forest ecosystems, water and soil protection, vegetation restoration and so on. Although previous studies have shown that this order has a very high species diversity in China, there are few reports on the species diversity of boletus in Jiangxi Province, China. We have systematically investigated the macrofungi diversity of Jiangxi Wuyishan National Nature Reserve for five years, where the natural conditions is excellent, the ecological environment is well protected and the diversity of macrofungi is abundant. Based on morphological and phylogenetic analyses, we described 6 new boletus species, i.e. Austroboletus albus, Tapinella qixianensis, Xanthoconium violaceipes, Xanthoconium violaceofuscum, Xerocomus rutilans and Xerocomus subsplendidus. All the new species were described and illustrated in detail.
Brief Cognitive Behavior Therapy for Stigmatization, Depression, Quality of life, social support and Adherence to Treatment among Patients with HIV/AIDS: A Randomized Control Trial
Authors: Abbas Qasir , Nisa Mehru , Khan Muhammad Umar , Anwar Nida , Aljhani Sumayah , Ramzan Zoobia , Shahzadi Mafia 
Published: May 2023

Abstract
Objective: Individuals living with HIV/AIDs are at high risk of mental health problems, particularly depression, stigma, quality of life, adherence to treatment, and lack of social support. The present study aimed to investigate the impact of brief-cognitive behavior therapy (B-CBT) on reducing depression and stigma and improving treatment adherence, quality of life, and social support among patients with HIV/AIDS attending antiretroviral therapy (ART).
Materials and Methods: This randomized clinical trial was conducted at ART Clinic in the Tehsil Headquarters Hospital Shahkot Nankana Sahib from July 2021 to October 2021. After baseline screening, 126 patients met the eligibility criteria and were allocated to the experimental group (EXPg=63) and waitlist-control group (WLCg=63). Participants’ age range was 20 to 55 years. Participants attending ART treatment were enrolled for the CBT treatment. Before this, all the participants completed a baseline assessment to ensure a level of severity and diagnosis. A total of eight therapeutic sessions based on CBT were conducted individually with EXPg. To assess the outcomes among patients receiving ART, we used the i.e., Demographic form, Patient health questionnaire, HIV stigma scale, General medication adherence scale, Multidimensional scale of perceived social support, and WHOQOL BREF scale.
Results: Findings reported B-CBT significantly reduced the level of depression (i.e. F (1, 78) = 101.38, p<.000, η2= .599), and social stigma (i.e. F (1, 78) = 208.47, p<.000, η2= .787) among patients with HIV/AIDS. Furthermore, CBT substantially improved the level of adherence to treatment (i.e. F(1,78)= 24.75, p<.000, η2= .503), social support (i.e. F (1, 78) = 128.33, p<.000, η2= .606), and quality of life (i.e. F (1, 78) = 373.39, p<.000, η2= .837) among patients with HIV/AIDS. Significant mean difference M(SD) on PHQ at post-analysis in the EXPg vs. WLC was seen 1.22(0.47) vs. 2.30(0.68) and similarly, on MPSS at a post-analysis in the EXPg vs. WLC 2.85(0.36) vs. 1.70(0.51) which indicates sound therapeutic outcomes.
Conclusions: Cognitive behavioral therapy effectively decreases the level of depression and stigma and enhances the level of social support, quality of life, and adherence to treatment among HIV/AIDS patients. It is concluded that cognitive behavior therapy is an effective treatment approach for patients with HIV/AIDS.
Trial Registration: Thai clinical trial registry (i.e. TCTR= TCTR20210702002, with URL: https://www.thaiclinicaltrials.org/show/TCTR20210702002).
The critical role of MnCeOx molecular dispersion in harsh reaction conditions for VOC treatment
Authors: Sim Ley Boon , Wu Zibao , Zhang Chi , Liu Xianghua , Fu Jile , Zhang Nuowei , Wang Jiexiang , Ye Songshou , Zheng Jinbao , Chen Binghui 
Published: May 2023

Abstract
The stability of catalysts perhaps is the most important issue for catalysis whatever fundamental or application. This study evaluates the stability of MnOx-CeO2 catalysts in the oxidation of ethyl acetate by comparing the performance of fresh catalysts with those treated under high-temperature hydrothermal conditions (750 ℃ and 5% steam for 12 h). Three different methods, namely redox-precipitation (RH), co-precipitation (CP), and impregnation (IP), were used to synthesize the catalysts and adjust the Mn-Ce dispersion. The activity difference between the fresh and treated catalysts decreased in the following order: RH-MnCe > CP-MnCe > IP-MnCe. Based on detailed characterizations and density functional theory calculations, the molecular dispersion of MnCeOx in RH-MnCe is key to ensuring the collapse resistance of Ce-doped Mn3O4, which yields the highest activity during ethyl acetate oxidation. This finding is supported by experiments conducted on RH-MnCe with different Mn/Ce ratios. Although the introduction of Ce can enhance the stability of Mn3O4, it must be carefully controlled at a moderate level to enable recycling between MnO2/Mn3O4.
Reluctance and loss of progeny fitness when relatives mated: A case study in a predatory ladybird, Propylea dissecta
Authors: Pervez Ahmad , Khan Moina 
Published: May 2023

Abstract
We investigated mating reluctance with relatives and its influence on the quantitative progeny and its fitness using a predatory ladybird, Propylea dissecta. For this purpose, we compared mating behaviour, mating duration and post-mating response in the form of fecundity, egg-viability, progeny-development, weight and fitness when mating occurred between relatives (father-daughter, mother-son, and siblings) with unrelated ladybird couples. Females of P. dissecta, particularly those belonging to earlier (mother) or later generation (daughter) exhibited strong mating reluctance than those of same generation (sibling) relatives. This reluctance was lacking in unrelated female ladybirds. A similar pattern was reflected in the mating duration and post-mating responses, as unrelated females mated for the longest duration with high fecundity, oviposition period and percentage of egg viability. Furthermore, the first progeny suffered the implication of mating with relatives in the form of delayed development, lighter developmental stages and reduced fitness with those experiencing maximum whose parents were not of the same generation. These parameters were optimal in the progeny of unrelated parents. These results alarm the process of mass-rearing, where the ladybirds are augmented giving no consideration to the parental line and allowing mating between relatives, which may result in inbreeding depression, thereby deteriorating the quality of progeny and impeding the biocontrol programmes.
MicroRNA-595: A novel circulating biomarker for prediction the status of cerebral collateral circulation in acute ischemic stroke
Authors: Wu Danhong , Wen Zhuoyu , Shao Rongrong , Cheng Kaili , Shen Liwei , Liu Wenpeng 
Published: May 2023

Abstract
Objective
This study aimed to investigate the correlation between the circulating miR-595 level and the status of cerebral collateral circulation (CCC), and to investigate whether miR-595 could serve as a potential biomarker for the status of CCC in acute ischemic stroke patients.
Material and methods
This prospective study consecutively enrolled acute ischemic stroke patients and the grouping was as followed according to ASPECTS system: patients with grades > 8 (benign CCC group) and patients with grades ≤ 8 (poor CCC group). Plasma miR-595 was measured by RT-PCR. Spearman rank correlation analysis and logistic regression analysis were used to examine associations between circulating miR-595 level and CCC. Receiver operating characteristic (ROC) curves were performed to determine circulating miR-595 cutoff level scores.
Results
106 patients were enrolled (51 with benign CCC and 55 with poor CCC). Plasma miR-595 was significantly higher in the benign CCC group compared with poor CCC group (P < 0.05). miR-595 was positively correlated with ASPECTS system (r = 0.567, P < 0.001), and negatively correlated with NIHSS on admission (r =-0.301, P = 0.002). Patients with higher circulating miR-595 level had a higher risk of benign CCC (OR = 1.122, 95% CI, 1.034,1.218) after adjusting traditional risk factors. ROC curves revealed the areas under the curve for circulating miR-595 were 0.8431 (95% CI 0.533–0.793).
Conclusions
This study indicated that plasma miR-595 level might be a novel potential prognostic biomarker for benign CCC after acute ischemic stroke onset, which might assist in the administration of thrombolysis and accurately preclinical assessment.