bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)
Authors: Weng Zongkuan , Deng Yitong , Tang Fen , Zhao Lukuan , Zhao Lingxiao , Wang Yuan , Dai Xibin , Zhou Zhilin , Cao Qinghe 
Published: August 2023

Abstract
Background
Sweetpotato is an important vegetable and food crop that is bred through sexual crosses and systematic selection. The use of in vitro germination of sweetpotato pollen to test its viability has important theoretical and practical implications for improving the efficiency of sweetpotato crossbreeding by controlling pollination and conducting research on sweetpotato pollen biology.

Results
In this study, we observed the morphological structure of sweetpotato pollen under a scanning electron microscope (SEM), developed an effective method for the in vitro germination of sweetpotato pollen, and examined the viability of sweetpotato pollen after treating plants at different temperatures before blossoming. Sweetpotato pollen grains are spherical, with an average diameter of 87.07 ± 3.27 μm (excluding spines), with multiple germination pores and reticulate pollen surface sculpture. We applied numerous media to sweetpotato pollen germination in vitro to screen the initial medium and optimised the medium components through single-factor design. The most effective liquid medium for in vitro sweetpotato pollen germination contained 50 g/L Sucrose, 50 g/L Polyethylene glycol 4000 (PEG4000), 100 mg/L Boric acid and 300 mg/L Calcium nitrate, with a pH = 6.0. The optimum growth temperature for pollen development in sweetpotato was from 25 to 30 °C. Neither staining nor in situ germination could accurately determine the viability of sweetpotato pollen.

Conclusions
In vitro germination can be used to effectively determine sweetpotato pollen viability. The best liquid medium for in vitro germination of sweetpotato pollen contained 50 g/L Sucrose, 50 g/L Polyethylene glycol 4000 (PEG4000), 100 mg/L Boric acid and 300 mg/L Calcium nitrate, with the pH adjusted to 6.0. This study provides a reliable medium for the detection of sweetpotato pollen viability, which can provide a theoretical reference for sweetpotato genetics and breeding.
‘Single-Seed-SpeedBulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L.)
Authors: Kigoni Milcah , Choi Megan , Arbelaez Juan David 
Published: August 2023

Abstract
Background
In plant breeding, one of the most cost-effective and efficient ways to increase genetic gain is to reduce the breeding cycle time. In general, modern breeding methods for self-pollinated crops should strive to develop fixed lines at the lowest possible cost and in the minimum possible amount of time. Previous studies on spring oat (Avena sativa L.) showed that combining high plant density with limited soil fertility and moisture levels in a growth media like sand effectively decreases the time and cost of generating fixed single-seed descent lines. More recently, ‘speed breeding,’ or the exposure to prolonged photoperiod regimes of 22 h, has been shown to decrease flowering time in oat significantly. The goal of this study was to combine ‘speed breeding’ with high-density planting in a limited soil fertility media to reduce further the costs and time required to develop oat single-seed-descent lines.

Results
We grew oat plants at low density in potting-mix (control), high density in potting-mix (HD-soil), and high density in sand (HD-sand) under 16 and 22 h of day length. We observed that oat plants grown in HD-sand and exposed to 22 h day length reduced their flowering time by around 20 and 5 days on average compared to those grown in control conditions at 16 and 22 h, respectively. We also observed that 85% of plants grown at high density in sand produced a single seed when grown in bulk conditions. In contrast, only 40% of plants grown at high density in potting-mix produced a single seed.

Conclusions
Our novel protocol showed that oat plants grown in high-density bulks, using sand media and 22-hour day length, reduced their flowering time by 20 days compared to control conditions and produced plants with single seeds, following closely single-seed descent assumptions while significantly reducing labor costs and greenhouse space. This methodology can be deployed in oat breeding programs to help them accelerate their rate of genetic grain for multiple traits.
Nicotine dependence and associated factors among persons who use electronic e-cigarettes in Malaysia - an online survey
Authors: Sreeramareddy Chandrashekhar T. , Shroff Sameeha Misriya , Gunjal Shilpa 
Published: August 2023

Abstract
Background
Nicotine dependence, factors associated with dependence, and self-reported side effects among people who use e-cigarettes are scarce in developing countries.

Methods
A sample of 302 persons who currently use e-cigarettes was recruited from discussion forums on Reddit, Facebook, and the forum ‘lowyat’. The online Google form survey collected data on demographics, e-cigarette use, and the reasons, for cigarette smoking, Fagerstorm Test for Nicotine Dependence adapted for e-cigarettes (eFTND), and side effects experienced.

Results
The mean age was 25.5 years (6.5), 60.6% were males and 86% had higher education. About 47% were using e-cigarettes only, 27.8% were currently using dual products (both electronic and conventional cigarettes), and 25.2% had also smoked cigarettes in the past. ‘Less harmful than cigarettes’ (56.3%), ‘because I enjoy it’ (46.7%), and ‘it has a variety of flavors (40.4%) were the common reasons for e-cigarette use. The mean eFTND score was 3.9 (SD = 2.2), with a median of four side effects (IQR 3–6), sore or dry mouth/throat (41.4%), cough 33.4%, headache (20.5%), dizziness (16.2%) were commonly reported side effects. eFTND score and side effects were higher among persons using dual products. By multiple linear regression analysis, males (β = 0.56 95% CI 0.45, 1.05, p = 0.033), dual-use (β = 0.95 95% CI 0.34, 1.56, p < 0.003), and use of nicotine-containing e-cigarettes (β = 0.66 95% CI 0.07, 1.25 p = 0.024) had higher eFTND score.

Conclusion
Our findings of the study call for the placement of disclaimers about possible nicotine addiction and side effects of e-cigarette products.
Analysis of clinical and physical dosimetric factors that determine the outcome of severe acute radiation pneumonitis in lung cancer patients
Authors: Zhao Jing , Ma Chenying , Gan Guanghui , Xu Xiaoting , Zhou Juying 
Published: August 2023

Abstract
Objective
We conducted a retrospective statistical analysis of clinical and physical dosimetric factors of lung cancer patients who had previously undergone lung and/or mediastinal radiotherapy and died of or survived severe acute radiation pneumonitis (SARP). Our study was the first to reveal the heterogeneity in clinical factors, physical dosimetric factors, and SARP onset time that determined the clinical outcomes of lung cancer patients who developed SARP.

Materials and methods
The clinical characteristics, physical dosimetry factors, and SARP onset time of deceased and surviving patients were retrospectively analyzed. SPSS 20.0 was used for data analysis. Student’s t-test was used for intergroup comparison, and a Mann–Whitney U test was used for data with skewed distribution. Qualitative data were represented using frequencies (%), and Fisher’s exact test or χ2 test was used for intergroup comparison of nonparametric data. Binary logistic analysis was used for univariate and multivariate analyses. Differences with a P < 0.05 were considered statistically significant.

Results
Univariate analysis revealed that the potential predictors of SARP death were as follows: ipsilateral lung V5 and V30, contralateral lung V5, V10, and V30, total lung V5, V10, and V30, mean lung dose, mean heart dose, and maximum spinal cord dose. Multivariate analysis showed that ipsilateral lung V5 and total lung V5 were predictors that determined the final outcome of SARP patients. In addition, we analyzed the time from the completion of radiotherapy to SARP onset, and found significant difference between the two groups.

Conclusions
There was no decisive correlation between clinical characteristics and SARP outcome (i.e., death or survival) in lung radiotherapy patients. Ipsilateral lung V5 and total lung V5 were independent predictors of death in SARP patients.
Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer
Authors: Dadgar-Zankbar Leila , Shariati Aref , Bostanghadiri Narjess , Elahi Zahra , Mirkalantari Shiva , Razavi Shabnam , Kamali Fatemeh , Darban-Sarokhalil Davood 
Published: August 2023

AbstractBackgroundColorectal cancer (CRC) is one of the most common cancers all over the world, and dysbiosis in the gut microbiota may play a role in colorectal carcinogenesis.Bacteroides fragiliscan lead to tumorigenesis by changing signaling pathways, including the WNT/β-catenin pathway. Therefore, in the present study, we investigated the correlation between the enterotoxigenicB. fragilisamount and the expression of signaling pathway genes involved in CRC.Materials and methodsB. fragiliswas determined in 30 tumors and adjacent healthy tissues by the qPCR method. Next, the relationship between enterotoxigenicB. fragilisand the expression of signaling pathway genes, including CCND1, TP53, BCL2, BAX, WNT, TCF, AXIN, APC, and CTNNB1 was investigated. Additionally, possible correlations between clinicopathological features of the tumor samples and the abundance ofB. fragiliswere analyzed.ResultsThe results showed thatB. fragiliswas detected in 100% of tumor samples and 86% of healthy tissues. Additionally, enterotoxigenicB. fragiliscolonized 47% of all samples, andbft-1toxin was the most frequently found isotype among the samples. The analysis showed that the high level ofB. fragilishas a significant relationship with the high expression of AXIN, CTNNB1, and BCL2 genes. On the other hand, our results did not show any possible correlation between this bacterium and the clinicopathological features of the tumor sample.ConclusionB. fragilishad a higher abundance in the tumor samples than in healthy tissues, and this bacterium may lead to CRC by making changes in cellular signaling pathways and genes. Therefore, to better understand the physiological effects ofB. fragilison the inflammatory response and CRC, future research should focus on dissecting the molecular mechanisms by which this bacterium regulates cellular signaling pathways.
Exosome nanovesicles as potential biomarkers and immune checkpoint signaling modulators in lung cancer microenvironment: recent advances and emerging concepts
Authors: Khan Naushad Ahmad , Asim Mohammad , Biswas Kabir H. , Alansari Amani N , Saman Harman , Sarwar Mohammad Zahid , Osmonaliev Kudaibergen , Uddin Shahab 
Published: August 2023

AbstractLung cancer remains the leading cause of cancer-related deaths globally, and the survival rate remains low despite advances in diagnosis and treatment. The progression of lung cancer is a multifaceted and dynamic phenomenon that encompasses interplays among cancerous cells and their microenvironment, which incorporates immune cells. Exosomes, which are small membrane-bound vesicles, are released by numerous cell types in normal and stressful situations to allow communication between cells. Tumor-derived exosomes (TEXs) possess diverse neo-antigens and cargoes such as proteins, RNA, and DNA and have a unique molecular makeup reflecting tumor genetic complexity. TEXs contain both immunosuppressive and immunostimulatory factors and may play a role in immunomodulation by influencing innate and adaptive immune components. Moreover, they transmit signals that contribute to the progression of lung cancer by promoting metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, and immunosuppression. This makes them a valuable resource for investigating the immune environment of tumors, which could pave the way for the development of non-invasive biomarkers that could aid in the prognosis, diagnosis, and immunotherapy of lung cancer. While immune checkpoint inhibitor (ICI) immunotherapy has shown promising results in treating initial-stage cancers, most patients eventually develop adaptive resistance over time. Emerging evidence demonstrates that TEXs could serve as a prognostic biomarker for immunotherapeutic response and have a significant impact on both systemic immune suppression and tumor advancement. Therefore, understanding TEXs and their role in lung cancer tumorigenesis and their response to immunotherapies is an exciting research area and needs further investigation. This review highlights the role of TEXs as key contributors to the advancement of lung cancer and their clinical significance in lung immune-oncology, including their possible use as biomarkers for monitoring disease progression and prognosis, as well as emerging shreds of evidence regarding the possibility of using exosomes as targets to improve lung cancer therapy.
Use of simulation models when developing and testing hospital evacuation plans: a tool for improving emergency preparedness
Authors: Rådestad Monica , Holmgren Cecilia , Blidegård Ellinor Linde , Montán Kristina Lennquist 
Published: August 2023

Abstract
Background
In recent decades, analyses of hospitals evacuations have generated valuable knowledge. Unfortunately, these evacuation case studies often lack crucial details and policies that would be helpful in evacuation preparedness. The aim of this study was to use a simulation model to illustrate how it can aid emergency planners in the development, testing, and revising of hospitals evacuation plans. This study includes evacuation exercises at two emergency hospitals in Region Stockholm, Sweden.

Methods
A scientifically validated simulation system for “table top” exercises was used for interactive training of hospital medical staff, prehospital staff and collaborating agencies. All participants acted in their usual professionals’ roles. The exercises were run in real-time and mirrored actual hospital resources with the aid of moveable magnetic symbols illustrating patients, staff and transport, presented on whiteboards. During the exercises, observers and independent instructors documented actions taken and post-exercise surveys were conducted to obtain reactions and compare results.

Results
The simulation system allowed the emergency planner to test the whole evacuation process, making it possible to train and evaluate the important functions of management, coordination, and communication. Post-exercise surveys explored participants perception of the exercises. Analysis of open-ended questions included areas for improvement and resulted in five main categories: (1) management and liaison; (2) communication; (3) logistics; (4) medical care and patient prioritisation; and (5) resource utilisation.

Conclusions
This study has shown that “table top” exercises using a validated simulation system can serve to guide emergency planners when developing evacuation plans, procedures, and protocols as well in training of all medical staff. The system also served to train adaptive thinking, leadership, communication, and clarification of critical functions.
Cross-species oncogenomics offers insight into human muscle-invasive bladder cancer
Authors: Wong Kim , Abascal Federico , Ludwig Latasha , Aupperle-Lellbach Heike , Grassinger Julia , Wright Colin W. , Allison Simon J. , Pinder Emma , Phillips Roger M. , Romero Laura P. , Gal Arnon , Roady Patrick J. , Pires Isabel , Guscetti Franco , Munday John S. , Peleteiro Maria C. , Pinto Carlos A. , Carvalho Tânia , Cota João , Du Plessis Elizabeth C. , Constantino-Casas Fernando , Plog Stephanie , Moe Lars , de Brot Simone , Bemelmans Ingrid , Amorim Renée Laufer , Georgy Smitha R. , Prada Justina , del Pozo Jorge , Heimann Marianne , de Carvalho Nunes Louisiane , Simola Outi , Pazzi Paolo , Steyl Johan , Ubukata Rodrigo , Vajdovich Peter , Priestnall Simon L. , Suárez-Bonnet Alejandro , Roperto Franco , Millanta Francesca , Palmieri Chiara , Ortiz Ana L. , Barros Claudio S. L. , Gava Aldo , Söderström Minna E. , O’Donnell Marie , Klopfleisch Robert , Manrique-Rincón Andrea , Martincorena Inigo , Ferreira Ingrid , Arends Mark J. , Wood Geoffrey A. , Adams David J. , van der Weyden Louise 
Published: August 2023

AbstractBackgroundIn humans, muscle-invasive bladder cancer (MIBC) is highly aggressive and associated with a poor prognosis. With a high mutation load and large number of altered genes, strategies to delineate key driver events are necessary. Dogs and cats develop urothelial carcinoma (UC) with histological and clinical similarities to human MIBC. Cattle that graze on bracken fern also develop UC, associated with exposure to the carcinogen ptaquiloside. These species may represent relevant animal models of spontaneous and carcinogen-induced UC that can provide insight into human MIBC.ResultsWhole-exome sequencing of domestic canine (n = 87) and feline (n = 23) UC, and comparative analysis with human MIBC reveals a lower mutation rate in animal cases and the absence of APOBEC mutational signatures. A convergence of driver genes (ARID1A, KDM6A, TP53,FAT1, andNRAS) is discovered, along with common focally amplified and deleted genes involved in regulation of the cell cycle and chromatin remodelling. We identify mismatch repair deficiency in a subset of canine and feline UCs with biallelic inactivation ofMSH2. Bovine UC (n = 8) is distinctly different; we identify novel mutational signatures which are recapitulated in vitro in human urinary bladder UC cells treated with bracken fern extracts or purified ptaquiloside.ConclusionCanine and feline urinary bladder UC represent relevant models of MIBC in humans, and cross-species analysis can identify evolutionarily conserved driver genes. We characterize mutational signatures in bovine UC associated with bracken fern and ptaquiloside exposure, a human-linked cancer exposure. Our work demonstrates the relevance of cross-species comparative analysis in understanding both human and animal UC.
The With Or Without Olecranon K-wire (WOW OK) Trial of tension band wire fixation versus cerclage fixation without K-wires in displaced stable olecranon fractures: study protocol for a randomized controlled trial
Authors: Cornefjord Gustav , Kostogiannis Ioannis , Rogmark Cecilia , Jerrhag Daniel , Wenger Daniel 
Published: August 2023

Abstract
Background
Displaced olecranon fractures with a stable elbow joint are classified as Mayo type 2a or 2b and are commonly operated with tension band wiring, i.e. two K-wires and a cerclage. Retrospective studies have reported fewer reoperations and complications with cerclage fixation alone when compared to tension band wiring, though with similar long-term results. We decided to compare tension band wiring to cerclage fixation of displaced, stable olecranon fractures in adults in a randomized controlled trial.

Methods
All patients ≥ 18 years old with Mayo type 2a and 2b fractures presenting at Skåne University hospital will be eligible for study inclusion, unless exclusion criteria are met. Two hundred participants will be included and randomized 1:1 to cerclage fixation or tension band wiring.
Outpatient physiotherapist follow-up appointments will be scheduled at 2 and 6 weeks and at 3, 12, and 36 months at the Dept. of Orthopaedics. A lateral view radiograph of the elbow will be analysed at 6 months. The primary outcome of our study is the rate of reoperations. Secondary outcomes are complication rates, severity of complications, and patient-reported outcome measures (QuickDASH, Short Musculoskeletal Function Assessment, pain level, and patient satisfaction). The sample size was calculated to give 80% power for detecting a statistically significant difference in reoperation rates (with alpha-value 0.05), based on a previous retrospective study.

Discussion
Reoperation and complication rates after tension band wiring of olecranon fractures are high. Treatment of these injuries is debated, and several ongoing trials compare tension band wiring with plate fixation, suture fixation, and non-operative treatment. As data from retrospective studies indicate that cerclage fixation may be superior to tension band wiring, we see a need for a randomized controlled trial comparing these methods. The WOW-OK Trial aims to obtain level-1 evidence that may influence treatment choice for this type of fracture.

Trial registration
ClinicalTrials.gov NCT05657899. Registered on 16 November 2022. The trial complies with SPIRIT and CONSORT guidelines. The SPIRIT figure is found in Table 2.
Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition
Authors: Qin Song , Wang Kang , Gao Fengzheng , Ge Baosheng , Cui Hongli , Li Wenjun 
Published: August 2023

AbstractMicroalgal biomass represents a sustainable bioresource for various applications, such as food, nutraceuticals, pharmaceuticals, feed, and other bio-based products. For decades, its mass production has attracted widespread attention and interest. The process of microalgal biomass production involves several techniques, mainly cultivation, harvesting, drying, and pollution control. These techniques are often designed and optimized to meet optimal growth conditions for microalgae and to produce high-quality biomass at acceptable cost. Importantly, mass production techniques are important for producing a commercial product in sufficient amounts. However, it should not be overlooked that microalgal biotechnology still faces challenges, in particular the high cost of production, the lack of knowledge about biological contaminants and the challenge of loss of active ingredients during biomass production. These issues involve the research and development of low-cost, standardized, industrial-scale production equipment and the optimization of production processes, as well as the urgent need to increase the research on biological contaminants and microalgal active ingredients. This review systematically examines the global development of microalgal biotechnology for biomass production, with emphasis on the techniques of cultivation, harvesting, drying and control of biological contaminants, and discusses the challenges and strategies to further improve quality and reduce costs. Moreover, the current status of biomass production of some biotechnologically important species has been summarized, and the importance of improving microalgae-related standards for their commercial applications is noted.