bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy evaluation of a commercially available MCT enriched therapeutic diet on dogs with idiopathic epilepsy treated with zonisamide: a prospective, randomized, double-blinded, placebo-controlled, crossover dietary preliminary study
Authors: Nakatsuka Kazumasa , Zanghi Brian , Hasegawa Daisuke 
Published: September 2023

Abstract
Background
Idiopathic epilepsy (IE) is a common, chronic brain dysfunction in dogs. Recently, the effect of feeding a diet enriched with medium-chain triglycerides (MCTs) on seizure frequency has been evaluated in several studies in dogs with IE. However, most dogs with IE in previous studies were treated with phenobarbital as the main antiseizure medication (ASM). In Japan, zonisamide (ZNS) is the most prescribed ASM for dogs with IE. The interaction between ZNS and various nutrients including MCTs and the potential effects on treatment efficacy resulting from combining these therapies have not been previously studied. A prospective, randomized, double-blinded, placebo-controlled, crossover dietary study was conducted. Dogs (n = 7) treated with ZNS were fed either a placebo diet (PL) or Purina ProPlan Veterinary Diet NeuroCare (NC) for 3 months, after which treatments were crossed over and continued for another 3 months. Seizure frequency (seizures/month; sz/m), blood tests including concentrations of ZNS and β-hydroxybutyric acid, and owner’s visual analogue scale score were collected from all dogs for both treatment periods.

Results
There was no significant difference in the seizure frequency between PL (2.95 ± 0.80 sz/m) and NC (1.90 ± 0.57 sz/m) during the 6 months of trial. Three of 7 dogs showed ≥ 50% seizure reduction, and 1 of those 3 dogs achieved seizure freedom in NC period. However, 2 of 7 dogs had no changes in epileptic seizure frequency, 2 of 7 dogs had a deterioration in seizure frequency in the NC period. Feeding the MCT diet concurrent with ZNS showed no apparent adverse effects and did not affect ZNS concentration.

Conclusions
This study indicated that the commercially available MCT-enriched diet (NC) can be safely used concurrently with ZNS for dogs with IE.
Damage-associated molecular patterns and fibrinolysis perturbation are associated with lethal outcomes in traumatic injury
Authors: Shimono Kenshin , Ito Takashi , Kamikokuryo Chinatsu , Niiyama Shuhei , Yamada Shingo , Onishi Hirokazu , Yoshihara Hideaki , Maruyama Ikuro , Kakihana Yasuyuki 
Published: September 2023

Abstract
Background
Upon cellular injury, damage-associated molecular patterns (DAMPs) are released into the extracellular space and evoke proinflammatory and prothrombotic responses in animal models of sterile inflammation. However, in clinical settings, the dynamics of DAMP levels after trauma and links between DAMPs and trauma-associated coagulopathy remain largely undetermined.

Methods
Thirty-one patients with severe trauma, who were transferred to Kagoshima City Hospital between June 2018 and December 2019, were consecutively enrolled in this study. Blood samples were taken at the time of delivery, and 6 and 12 h after the injury, and once daily thereafter. The time-dependent changes of coagulation/fibrinolysis markers, including thrombin-antithrombin complex, α2-plasmin inhibitor (α2-PI), plasmin-α2-PI complex, and plasminogen activator inhibitor-1 (PAI-1), and DAMPs, including high mobility group box 1 and histone H3, were analyzed. The relationship between coagulation/fibrinolysis markers, DAMPs, Injury Severity Score, in-hospital death, and amount of blood transfusion were analyzed.

Results
The activation of coagulation/fibrinolysis pathways was evident at the time of delivery. In contrast, PAI-1 levels remained low at the time of delivery, and then were elevated at 6–12 h after traumatic injury. Histone H3 and high mobility group box 1 levels were elevated at admission, and gradually subsided over time. PAI-1 levels at 6 h were associated with serum histone H3 levels at admission. Increased histone H3 levels and plasmin-α2-PI complex levels were associated with in-hospital mortality. α2-PI levels at admission showed the strongest negative correlation with the amount of blood transfusion.

Conclusion
The elevation of histone H3 levels and fibrinolysis perturbation are associated with fatal outcomes in patients with traumatic injury. Patients with low α2-PI levels at admission tend to require blood transfusion.
Impact of MRI on target volume definition in head and neck cancer patients
Authors: Clasen Kerstin , Nachbar Marcel , Gatidis Sergios , Zips Daniel , Thorwarth Daniela , Welz Stefan 
Published: September 2023

Abstract
Background
Target volume definition for curative radiochemotherapy in head and neck cancer is crucial since the predominant recurrence pattern is local. Additional diagnostic imaging like MRI is increasingly used, yet it is usually hampered by different patient positioning compared to radiotherapy. In this study, we investigated the impact of diagnostic MRI in treatment position for target volume delineation.

Methods
We prospectively analyzed patients who were suitable and agreed to undergo an MRI in treatment position with immobilization devices prior to radiotherapy planning from 2017 to 2019. Target volume delineation for the primary tumor was first performed using all available information except for the MRI and subsequently with additional consideration of the co-registered MRI. The derived volumes were compared by subjective visual judgment and by quantitative mathematical methods.

Results
Sixteen patients were included and underwent the planning CT, MRI and subsequent definitive radiochemotherapy. In 69% of the patients, there were visually relevant changes to the gross tumor volume (GTV) by use of the MRI. In 44%, the GTV_MRI would not have been covered completely by the planning target volume (PTV) of the CT-only contour. Yet, median Hausdorff und DSI values did not reflect these differences. The 3-year local control rate was 94%.

Conclusions
Adding a diagnostic MRI in RT treatment position is feasible and results in relevant changes in target volumes in the majority of patients.
Circ_0001825 promotes osteogenic differentiation in human-derived mesenchymal stem cells via miR-1270/SMAD5 axis
Authors: Zheng Changjun , Ding Lingzhi , Xiang Ziming , Feng Mingxuan , Zhao Fujiang , Zhou Zhaoxin , She Chang 
Published: September 2023

Abstract
Background
The implication of deregulated circular RNAs in osteoporosis (OP) has gradually been proposed. Herein, we aimed to study the function and mechanism of circ_0001825 in OP using osteogenic-induced human-derived mesenchymal stem cells (hMSCs).

Methods
The content of genes and proteins was tested by quantitative real-time polymerase chain reaction and Western blotting. The osteogenic differentiation in hMSCs were evaluated by ALP activity and Alizarin Red staining, as well as the detection of osteogenesis-related markers. Cell viability and apoptosis were measured by CCK-8 assay and flow cytometry. The binding between miR-1270 and circ_0001825 or SMAD5 (SMAD Family Member 5) was confirmed by using dual-luciferase reporter assay and pull-down assay.

Results
Circ_0001825 was lowly expressed in OP patients and osteogenic induced hMSCs. Knockdown of circ_0001825 suppressed hMSC viability and osteogenic differentiation, while circ_0001825 overexpression showed the exact opposite effects. Mechanistically, circ_0001825/miR-1270/SMAD5 formed a feedback loop. MiR-1270 was increased and SMAD5 was decreased in OP patients and osteogenic induced hMSCs. MiR-1270 up-regulation suppressed hMSC viability and osteogenic differentiation, which was reversed by SMAD5 overexpression. Moreover, miR-1270 deficiency abolished the effects of circ_0001825 knockdown on hMSCs.

Conclusion
Circ_0001825 promoted hMSC viability and osteogenic differentiation via miR-1270/SMAD5 axis, suggesting the potential involvement of circ_0001825 in osteoporosis.
Schisandrin protects against ulcerative colitis by inhibiting the SGK1/NLRP3 signaling pathway and reshaping gut microbiota in mice
Authors: Wang Xiaohu , Shen Chaozhuang , Wang Xingwen , Tang Jin , Wu Zijing , Huang Yunzhe , Shao Wenxin , Geng Kuo , Xie Haitang , Pu Zhichen 
Published: September 2023

Abstract
Background
According to the Chinese Pharmacopoeia, the fruit of Schisandra chinensis (Turcz.) Baill. (SC) is an important traditional Chinese medicine that can be used to treat diarrhea. Despite the increasing research on the anti-inflammatory and anti-oxidant aspects of SC, the studies on the anti-ulcerative colitis of Schisandrin (SCH), the main constituent of SC, are relatively few.

Methods
The mice used in the study were randomly distributed into 6 groups: control, model, 5-ASA, and SCH (20, 40, 80 mg/kg/d). The mice in the model group were administered 3% (w/v) dextran sulfate sodium (DSS) through drinking water for 7 days, and the various parameters of disease activity index (DAI) such as body weight loss, stool consistency, and gross blood were measured. ELISA was used to detect inflammatory factors, and bioinformatics combined with transcriptome analysis was done to screen and verify relevant targets. 16S rDNA high-throughput sequencing was used to analyze the composition of the gut microbiota(GM), while mass spectrometry was done to analyze the changes in the content of bile acids (BAs) in the intestine.

Results
Mice treated with SCH experienced significant weight gain, effectively alleviating the severity of colitis, and decreasing the levels of inflammatory factors such as TNF-α, IL-1β, IL-18, IL-6, and other related proteins (NLRP3, Caspase-1, SGK1) in UC mice. Furthermore, the analysis of GM and BAs in mice revealed that SCH increased the relative abundance of Lactobacilli spp, reduced the relative abundance of Bacteroides, and promoted the conversion of primary BAs to secondary BAs. These effects contributed to a significant improvement in the DSS-induced GM imbalance and the maintenance of intestinal homeostasis.

Conclusion
It seems that there is a close relationship between the SCH mechanism and the regulation of SGK1/NLRP3 pathway and the restoration of GM balance. Therefore, it can be concluded that SCH could be a potential drug for the treatment of UC.
Analysis of the accuracy and reliability of vertical jump evaluation using a low-cost acquisition system
Authors: Caseiro-Filho Luis C. , Girasol Carlos E. , Rinaldi Mateus L. , Lemos Tenysson W. , Guirro Rinaldo R. J. 
Published: September 2023

Abstract
Background
The vertical jump can be analyzed based on the flight time achieved by the individual. This measurement can be obtained using a force platform or a three-dimensional infrared camera system, but such equipment is expensive and requires training for data collection and processing. Thus, this study aimed to evaluate the accuracy and reliability of using a smartphone and the Kinovea software compared with a force platform as a method of vertical jump analysis.

Methods
For this purpose, two independent evaluators analyzed videos of bipodal and unipodal vertical jumps by counting the variables among participants. The participants performed three consecutive jumps in bipodal and unipodal conditions with the dominant and non-dominant legs.

Results
The intra-rater analysis for bipodal jumps was found to have excellent reproducibility (ICC = 0.903 to 0.934), whereas for unipodal jumps, the reproducibility was moderate to excellent (ICC = 0.713 to 0.902). The inter-rater analysis showed that for bipodal jumps, the reproducibility is substantial to excellent (ICC = 0.823 to 0.926), while for unipodal jumps, it is moderate (ICC = 0.554 to 0.702).

Conclusions
Therefore, it can be concluded that the vertical jump evaluation can be performed using the smartphone-Kinovea system. However, the same evaluator should carry out the evaluation to maintain reliable indices.
Seed nutrient is more stable than leaf in response to changing multiple resources in an alpine meadow
Authors: Li Jiapu , Tian Dashuan , Yu Kailiang , Guo Hongbo , Zhang Ruiyang , Wang Jinsong , Zhou Qingping , Niu Shuli 
Published: September 2023

Abstract
Background
It has been long thought that nitrogen (N), phosphorus (P) concentrations and their ratios (N:P) in metabolically active or functional organs (i.e., leaves) are less responsive to environmental changes. Little attention, however, has been paid to the reproductive organs—seeds, while seeds may maintain their nutrients more stable for the evolutionary fitness of next generation.

Methods
Here, we conducted a field experiment of N, P addition and drought in an alpine meadow, aiming to compare the difference of leaf and seed nutrients and stoichiometric ratios in response to these resource treatments and their interactions. Four dominant species were selected among grass and forb functional groups, including Elymus nutans, Deschampsia caespitosa, Artemisia roxburghiana and Polygonum viviparum.

Results
Under natural conditions, leaf N and P concentrations were consistently lower than seed among species. However, leaf nutrients were much more sensitive than seed nutrients to N and P addition. Specifically, N or P addition accordingly increased leaf N or P concentration by 22.20–44.24% and 85.54–93.61%, while only enhanced seed N or P concentration by 5.15–17.20% and 15.17–32.72%, respectively. Leaf N or P concentration was significantly reduced by P or N addition, but seed nutrients remained unchanged. In contrast, drought did not change both organ nutrients. Similarly, nutrient addition and drought had synergistic interactions on leaf nutrients, but not on seed nutrients.

Conclusions
This study highlights that seed nutrient concentrations could be more stable than metabolically active leaf organ when facing multidimensional resource changes. This complements the traditional view on the ‘Stable Leaf Nutrient Hypothesis’ with the involvement of reproductive organs. The less responsiveness of seed nutrients suggests the adaptive strategy to ensure the success of next generations and long-term plant demographic stability.
Alexithymia characteristics are associated with salience network activity in healthy participants: an arterial spin labeling study
Authors: Motomura Yuki , Fukuzaki Ayaka , Eto Sanami , Hirabayashi Naoki , Gondo Motoharu , Izuno Satoshi , Togao Osamu , Yamashita Koji , Kikuchi Kazufumi , Sudo Nobuyuki , Yoshihara Kazufumi 
Published: September 2023

Abstract
Background
Alexithymia, a personality trait characterized by difficulties in identifying and expressing their emotions despite having a range of emotional experiences, can impact individuals’ stress coping mechanisms. While many studies have investigated brain functions associated with specific tasks in relation to emotion processing, research focusing on resting-state brain functions has been limited. Thus, the aim of this study was to investigate the relationship between alexithymia and brain function by analyzing arterial spin labeling (ASL) data obtained during the resting state.

Methods
A brain structural and functional imaging study was conducted on 42 healthy adult men and women using ASL and the 20-item Toronto Alexithymia Scale (TAS-20) questionnaire survey. Cerebral blood flow and functional connectivity values were calculated for regions of interest in the default mode network, saliency network, and central executive network from the ASL data. Correlation analysis was performed with TAS20 scores, and partial correlation analysis was conducted to control for anxiety and depression.

Results
The functional connectivity analysis revealed a negative correlation between the functional connectivity of the right insular cortex and left anterior cingulate cortex and the total score of TAS, as well as difficulty identifying feelings and difficulty describing feeling subscores, indicating that the higher the scores, the weaker the functional connectivity between these regions (T = -3.830, p = 0.0013, R = -0.5180). This correlation remained significant even after controlling for anxiety and depression using partial correlation analysis.

Conclusion
The present study revealed differences in the activity of the Saliency Network at rest as measured by ASL, which were independent of anxiety and depression, and varied depending on the severity of alexithymia. This functional change may underlie the neural basis of decreased emotional processing observed in alexithymia. These findings may contribute to the elucidation of the neural mechanisms of alexithymia, which can lead to social impairments, and suggest the usefulness of ASL measurement as a biomarker of alexithymia.
Antipsychotic effects on anthropometric outcomes in anorexia nervosa: a retrospective chart review of hospitalized children and adolescents
Authors: Frank Bettina , Arnold Sabine , Jaite Charlotte , Correll Christoph U. 
Published: September 2023

Abstract
Background
Off-label antipsychotic use is not uncommon in youth with anorexia nervosa (AN), aiming to enhance suboptimal weight restoration, yet its efficacy remains debated, especially in youth.

Methods
Retrospective chart review of consecutively admitted inpatients (ages 8–18 years) with restricting/binge-purge AN, comparing youth with versus without antipsychotic treatment regarding baseline factors, treatment, and anthropometric outcome characteristics including all patients and matched subgroups. Matched subsamples were also compared regarding faster versus slower weight change (median split). Furthermore, within-subject analyses compared weight gain trajectories before versus after antipsychotic use in antipsychotic-treated youth. These results were then compared in a pre-/post design with the matched control group without antipsychotic treatment, using the mean duration until antipsychotic use in the antipsychotic-treated group as the dividing timeline, controlling for a potential order effect, in that later rather than earlier antipsychotic treatment for AN may be more successful.

Results
Of 294 youth with AN (median age = 15.2 (interquartile range = 14.0, 16.6) years, females = 96.6%, restricting subtype = 81.0%, hospitalization duration = 98.2 ± 43.2 days), 44 (15.0%) underwent 52 antipsychotic trials (olanzapine = 63.5%). In multivariable analyses, antipsychotic use was independently associated with younger age, childhood physical abuse history, comorbid borderline personality traits, and lower pre-antipsychotic weight gain (p < 0.0001). In unmatched groups, antipsychotic-treated versus non-treated youth had significantly lower discharge anthropometric parameters, longer inpatient treatment, and lower weight change/week (p < 0.001–p = 0.005), without significant differences between olanzapine and non-olanzapine antipsychotics (p = 0.27–0.44). Non-significant antipsychotic effects on weight outcomes were confirmed in (1) matched subgroups of antipsychotic-treated versus non-treated youth (n = 43 each), (2) youth with faster versus slower weight gain (n = 21 vs. n = 22), and (3) antipsychotic-treated youth when comparing weight change before versus after antipsychotic use (n = 31). Moreover, in antipsychotic-treated youth, weight change/week remained significantly lower versus matched non-antipsychotic-treated youth (n = 31) both before (p = 0.053) and after (p = 0.006) the median time (5 weeks) until antipsychotic use.

Conclusions
In this naturalistic study, clinician's antipsychotic choice, given to a more severely ill subgroup of adolescents with AN, did not significantly improve overall worse weight change trajectories during inpatient treatment, even in matched subgroups. Randomized trials in individuals reflecting real-world samples are needed to evaluate the utility of antipsychotic treatment in youth with AN.
Slow slip event in the focal area of the 1975 Kurile tsunami earthquake inferred from unusual long-term seismic quiescence
Authors: Katsumata Kei 
Published: September 2023

AbstractIn subduction zones, slow slip events (SSEs) have been observed in the portion deeper than the downdip edge of seismogenic zone. However, since it is far offshore from geodetic networks on land, shallow SSEs near the trench axis are hardly observed. Despite of less quantitative than seafloor geodetic observations, a method for inferring the shallow SSEs based on seismic quiescence was presented in this study. Unusual decrease in occurrence rate of M ≥ 5.0 earthquakes was found in the southwestern Kurile Islands. The occurrence rate was ∼1.3 events/year between 1977 and around 2004 and no earthquake was observed during 16 years after 2004. The spatial pattern of the seismic quiescence can be explained qualitatively by the Coulomb failure stress change due to shallow SSE and its fault plane is on the upper boundary of the subducting Pacific plate in the focal area of the 1975 tsunami earthquake.
Modeling geomagnetic spikes: the Levantine Iron Age anomaly
Authors: Rivera Pablo , Pavón-Carrasco F. Javier , Osete María Luisa 
Published: September 2023

AbstractThe Levantine Iron Age anomaly (LIAA) is a regional short-decadal geomagnetic strength field variation located at the Levantine region characterized by high intensities with maximum virtual axial dipole moments around 190 ZAm2. It has been constrained by archeomagnetic data coming from Eastern Europe and Western Asia between 1050 and 700 BC. The LIAA can be related to a fast and spatially localized geomagnetic positive anomaly (spike) at the Earth’s surface. In this study, we model the LIAA by using a Fisher–von Mises function that fits the most recent archeomagnetic intensity database in the region. A spherical harmonic analysis is implemented for this spike function to perturb a base model in order to build a global reconstruction (perturbed-model) that reproduces the spatial and temporal characteristics of the LIAA. Our results show the importance of harmonic degrees from n = 3–4 to n = 20 to reconstruct the anomaly extension suggested by the database. Two maxima linked with the LIAA are reproduced by our global perturbed-model at the Levantine region at 950 BC and 750 BC. A third maxima in intensity around 500 BC is also observed, affecting the whole Europe.
Graphical Abstract