bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a method to diagnose endometrial cancer based on the AuNRs‐AntiVimentin optical probe
Authors: Zhang Qingshun , Zhang Zhenwang , Zhang Hongbin , Wang Hui , Tang Quanlian , Wang Yufei , Gao Qiuye , Liu Jianhui , Liu Yi 
Published: May 2023

AbstractEndometrial cancer is one of the common gynecological malignancies, and its incidence has been increasing year by year in recent years, raising higher requirements for its rapid diagnosis. In this article, gold nanorods (AuNRs) with localized surface plasmon resonance properties (LSPR) has used to prepare AuNRs‐antibody to waveform protein (AuNRs‐AntiVimentin) optical probes, and a new method has been constructed that could rapidly detect and identify endometrial cancer tissue sections by polarized light microscopy. AuNRs were prepared by seed growth method using gold chloride as raw material, and the morphology of AuNRs and the optical characteristics of AuNRs‐AntiVimentin has characterized by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV–Vis), and zeta potential; immunohistochemistry (IHC) and AuNRs‐AntiVimentin optical probes have used to detect clinical endometrial cancer, respectively. The AuNRs‐AntiVimentin optical probe has been used to detect endometrial cancer tissue sections and found to have good bio‐specificity, with no significant difference in the detection of AuNRs‐AntiVimentin compared with conventional IHC techniques (p > .05). An optical probe generated by coupling AuNRs with Vimentin antibodies has been obtained to detect and identify endometrial cancer with simple operation and comparable effect to conventional IHC, providing a new method and idea for the rapid detection of endometrial cancer.
Predictors of target lesion failure after treatment of left main, bifurcation, or chronic total occlusion lesions with ultrathin‐strut drug‐eluting coronary stents in the ULTRA registry
Authors: de Filippo Ovidio , Bruno Francesco , Pinxterhuis Tineke H. , Gąsior Mariusz , Perl Leor , Gaido Luca , Tuttolomondo Domenico , Greco Antonio , Verardi Roberto , Lo Martire Gianluca , Iannaccone Mario , Leone Attilio , Liccardo Gaetano , Caglioni Serena , González Ferreiro Rocio , Rodinò Giulio , Musumeci Giuseppe , Patti Giuseppe , Borzillo Irene , Tarantini Giuseppe , Wańha Wojciech , Casella Bruno , Ploumen Eline H. , Pyka Łukasz , Kornowski Ran , Gagnor Andrea , Piccolo Raffaele , Roubin Sergio Raposeiras , Capodanno Davide , Zocca Paolo , Conrotto Federico , De Ferrari Gaetano M. , von Birgelen Clemens , D'Ascenzo Fabrizio ,   ULTRA Collaborators
Published: May 2023

AbstractBackgroundData about the long‐term performance of new‐generation ultrathin‐strut drug‐eluting stents (DES) in challenging coronary lesions, such as left main (LM), bifurcation, and chronic total occlusion (CTO) lesions are scant.MethodsThe international multicenter retrospective observational ULTRA study included consecutive patients treated from September 2016 to August 2021 with ultrathin‐strut (<70 µm) DES in challenging de novo lesions. Primary endpoint was target lesion failure (TLF): composite of cardiac death, target‐lesion revascularization (TLR), target‐vessel myocardial infarction (TVMI), or definite stent thrombosis (ST). Secondary endpoints included all‐cause death, acute myocardial infarction (AMI), target vessel revascularization, and TLF components. TLF predictors were assessed with Cox multivariable analysis.ResultsOf 1801 patients (age: 66.6 ± 11.2 years; male: 1410 [78.3%]), 170 (9.4%) experienced TLF during follow‐up of 3.1 ± 1.4 years. In patients with LM, CTO, and bifurcation lesions, TLF rates were 13.5%, 9.9%, and 8.9%, respectively. Overall, 160 (8.9%) patients died (74 [4.1%] from cardiac causes). AMI and TVMI rates were 6.0% and 3.2%, respectively. ST occurred in 11 (1.1%) patients while 77 (4.3%) underwent TLR. Multivariable analysis identified the following predictors of TLF: age, STEMI with cardiogenic shock, impaired left ventricular ejection fraction, diabetes, and renal dysfunction. Among the procedural variables, total stent length increased TLF risk (HR: 1.01, 95% CI: 1−1.02 per mm increase), while intracoronary imaging reduced the risk substantially (HR: 0.35, 95% CI: 0.12−0.82).ConclusionsUltrathin‐strut DES showed high efficacy and satisfactory safety, even in patients with challenging coronary lesions. Yet, despite using contemporary gold‐standard DES, the association persisted between established patient‐ and procedure‐related features of risk and impaired 3‐year clinical outcome.
Manufacturing of a Burner Plate by Diffusion Bonding to Investigate Premixed Fuel‐Rich Oxy‐Fuel Flames at Increased Pressure and Preheating
Authors: Gietzelt Thomas , Sentko Matthias M. , Stelzner Björn , Trimis Dimosthenis 
Published: May 2023

AbstractCombustion of hydrocarbons with pure oxygen as oxidizer is used, e.g., in high‐temperature processes such as the partial oxidation (POX) of hydrocarbons to produce synthesis gas of high purity. Due to the prevailing temperatures, active cooling is required for many parts. For laboratory‐scale experiments, the dimensions of key parts are too small for conventional manufacturing processes. One example is the manufacturing of a burner plate especially developed for POX processes. The complex geometries of several hundreds of burner nozzles and perpendicular cooling channels across the diameter of the burner plate cannot be manufactured in a conventional way. For this burner, the advantage of chemical etching of thin sheet material and stacking of multiple sheet layouts was used to assemble the layout of the burner. The burner plate was then diffusion‐bonded, allowing the complex design to be realized. The partial oxidation of CH4/O2 flames at the laboratory scale could thus be studied under industrially relevant conditions.
Evaluation of a handheld near‐infrared spectroscopy sensor for rapid corn kernel moisture estimation
Authors: Agyei B. , Andresen J. , Singh M. P. 
Published: May 2023

Core Ideas
Rapid and accurate estimation of corn kernel moisture in‐field is critical for timely harvests.
The NIRS sensor predicted corn kernel moisture with high accuracy (R2 ≥ .95) across a wide moisture range (15–60%).
Sensor accuracy was also consistent across multiple corn hybrids and environments.
A Template‐Free Pd2L4 Cage with up to Nanomolar Affinity for Chloride in Aqueous Solutions
Authors: Sivalingam Vellaiyadevan , Krishnaswamy Shobhana , Chand Dillip Kumar 
Published: May 2023

AbstractInvited for the cover of this issue is the group of Dillip Kumar Chand at the Indian Institute of Technology Madras. The image depicts the efficient chloride binding ability of a designer self‐assembled coordination cage in water. The binding occurs selectively even in presence of models of biologically abundant anionic systems. Read the full text of the article at 10.1002/chem.202300891.
Cerium‐promoted Ni/SiO2 catalyst for CO methanation
Authors: Hou Zhanggui , Chen Yiming , Ma Xin , Zhou Ling , Wang Wen , Qiu Jiesan , Zhang Yi 
Published: May 2023

AbstractCe‐promoted Ni catalysts were developed and applied in a CO methanation reaction. The 10%Ni/SiO2 catalyst exhibits poor initial CO conversion (32.8%) and rapid deactivation with the highest methane selectivity during CO the methanation reaction. In contrast, the 4%Ce–10%Ni/SiO2 catalyst shows dramatically increased initial CO conversion, which is up to 90.7%. Additionally, the apparent activation energy, Ea value, of 4%Ce–10%Ni/SiO2 was calculated to be 102.2 kJ/mol according to the Arrhenius equation, which is much lower than that of the 10%Ni/SiO2 catalyst, which was 139.1 kJ/mol. Based on various characterization results, it is found that the added Ce significantly improves the dispersion of the supported nickel, suppresses the sintering of nickel particles, and forms more adsorbed CO species of three‐fold carbonyl species, resulting in higher CO conversion and good stability during the CO methanation reaction.
PROTOCOL: Assessment of outcome reporting bias in studies included in Campbell systematic reviews
Authors: Littell Julia H. , Gorman Dennis M. , Valentine Jeffrey C. , Pigott Therese D. 
Published: May 2023

AbstractThis is the protocol for a Campbell systematic review. The objectives are as follows: To identify methods used to assess the risk of outcome reporting bias (ORB) in studies included in recent Campbell systematic reviews of intervention effects. The review will answer the following questions: What proportion of recent Campbell reviews included assessment of ORB? How did recent reviews define levels of risk of ORB (what categories, labels, and definitions did they use)? To what extent and how did these reviews use study protocols as sources of data on ORB? To what extent and how did reviews document reasons for judgments about risk of ORB? To what extent and how did reviews assess the inter‐rater reliability of ORB ratings? To what extent and how were issues of ORB considered in the review's abstract, plain language summary, and conclusions?
Separating Vibrational Coherences in Ground/Excited Electronic States of Solvent/Solute Following Non‐Resonant/Resonant Impulsive Excitation
Authors: Dhamija Shaina , Bhutani Garima , De Arijit K. 
Published: May 2023

AbstractIn a quest to track down the origin of coherent vibrational motions observed in femtosecond pump‐probe transients, whether they arise from ground/excited electronic state of solute or are contributed by the solvent, we demonstrate a method for extricating vibrations under resonant and non‐resonant impulsive excitations using a diatomic solute in condensed phase (iodine in carbon tetrachloride) with aid of spectral dispersion of the chirped broadband probe. Most importantly, we show how a sum over intensities for a select region of detection wavelengths and Fourier transform of data over select temporal window untwine contributions from vibrational modes of different origins. Thus, in a single pump‐probe experiment, vibrational features specific to solute as well as solvent are disentangled that are otherwise spectrally overlapping and are non‐separable in conventional (spontaneous/stimulated) Raman spectroscopy employing narrowband excitation. We envision wide‐ranging applications of this method to unveil vibrational features in complex molecular systems.
Calculation of Mechanical Properties, Electronic Properties, and Thermodynamic Properties of AuAl Crystal: First‐Principles Calculation
Authors: Han Xujin , Liu Manmen , Zhou Xiaolong 
Published: May 2023

AbstractThe structural stability, elastic properties, electronic properties, and thermodynamic property (Debye temperature) of AuAl intermetallic compound (IMC) are systematically investigated using first‐principles calculations based on density functional theory. The elastic constants, elastic moduli (including bulk modulus, shear modulus, Young's modulus, and Poisson's ratio) and Vickers hardness, the density of states, and Debye temperature of AuAl under hydrostatic pressure are calculated. The calculation results show that AuAl is an elastic anisotropic material with low rigidity and high toughness under zero pressure. Under hydrostatic pressure, the anisotropy index decreases while the hardness and Debye temperature increase. The research in this paper provides a theoretical basis for the study of the properties of bonding gold wire intermetallics.
A novel thieno[2,3‐d]pyrimidine derivative inhibiting vascular endothelial growth factor receptor‐2: A story of computer‐aided drug discovery
Authors: Sobh Eman A. , Dahab Mohammed A. , Elkaeed Eslam B. , Alsfouk Bshra A. , Ibrahim Ibrahim M. , Metwaly Ahmed M. , Eissa Ibrahim H. 
Published: May 2023

AbstractFollowing the pharmacophoric features of vascular endothelial growth factor receptor 2 (VEGFR‐2) inhibitors, a novel thieno[2,3‐d]pyrimidine derivative has been designed and its activity against VEGFR‐2 has been demonstrated by molecular docking studies that showed an accurate binding mode and an excellent binding energy. Furthermore, the recorded binding was confirmed by a series of molecular dynamics simulation studies, which also revealed precise energetic, conformational, and dynamic changes. Additionally, molecular mechanics with generalized Born and surface area solvation and polymer‐induced liquid precursors studies were conducted and verified the results of the MD simulations. Next, in silico absorption, distribution, metabolism, excretion, and toxicity studies have also been conducted to examine the general drug‐like nature of the designed candidate. According to the previous results, the thieno[2,3‐d]pyrimidine derivative was synthesized. Fascinatingly, it inhibited VEGFR‐2 (IC50 = 68.13 nM) and demonstrated strong inhibitory activity toward human liver (HepG2), and prostate (PC3) cell lines with IC50 values of 6.60 and 11.25 µM, respectively. As well, it was safe and showed a high selectivity index against normal cell lines (WI‐38). Finally, the thieno[2,3‐d]pyrimidine derivative arrested the growth of the HepG2 cells at the G2/M phase inducing both early and late apoptosis. These results were further confirmed through the ability of the thieno[2,3‐d]pyrimidine derivative to induce significant changes in the apoptotic genes levels of caspase‐3, caspase‐9, Bcl‐2 associated X‐protein, and B‐cell lymphoma 2.
Work related musculoskeletal disorders among dermatologists: A review
Authors: Jazzar Yara , Beidas Tala , Shadid Asem M. , Demasi Carolina , Sulaiman Mohammad Abdulaziz Al 
Published: May 2023

AbstractBackgroundWork Related Musculoskeletal Disorders (WRMSD's) is an entity that includes conditions and diseases that are caused or aggravated by a person's workplace conditions. Multiple studies have been published regarding WRMSD's affecting physicians among different specialties; however the prevalence and nature of WRMSD's affecting dermatologists is unknown.MethodsThe electronic database MEDLINE was searched through PubMed and the google scholar platform in February 2022 using the following search terms: “dermatologists AND work related musculoskeletal disorders”, “dermatologists AND ergonomics”, as well as “physicians AND work related musculoskeletal disorders”. All articles involving dermatologists or dermatology residents were included in this study.ResultsOur search revealed that WRMSD's among dermatologists were assessed in 11 Articles. However, Most literature addressing the topic of WRMSD's among dermatologists is focused on Mohs surgeons with only one article discussing dermatologists in general. The main complaints were pain and stiffness involving the neck, shoulders, and the lumbar area of the back, respectively.ConclusionDermatologists in general are more likely to develop WRMSD's due to the nature of their work and the procedures they perform. However, there are very few articles published regarding this matter, and consequently, little is known about the WRMDs that affect dermatologists and the ergonomic solutions that could be applied to prevent them.
Epigallocatechin gallate (EGCG) inhibits lipopolysaccharide‐induced inflammation in RAW 264.7 macrophage cells via modulating nuclear factor kappa‐light‐chain enhancer of activated B cells (NF‐κB) signaling pathway
Authors: Hossen Imam , Kaiqi Zhang , Hua Wu , Junsong Xiao , Mingquan Huang , Yanping Cao 
Published: May 2023

AbstractEpigallocatechin‐3‐gallate (EGCG) is a major bioactive compound in tea polyphenol extract. After ingestion, EGCG reaches the intestine and may commence anti‐inflammation in the intestinal organ. Thus, in this paper, the anti‐inflammatory effect of EGCG was studied using lipopolysaccharide (LPS)‐induced inflammation in RAW 264.7 cells. LPS induction instigated morphological deformation extensively which was normalized by EGCG. In LPS‐induced macrophage cells, EGCG was found to lower cellular nitric oxide (32% of LPS group) and intercellular ROS level (45.4% of LPS group). It also suppressed the expression of IL‐1β (LPS 132.6 ± 14.6, EGCG 10.67 ± 3.65), IL‐6 (LPS 2994.44 ± 178.5, EGCG 408.33 ± 52.34), TNF‐α (LPS 27.11 ± 2.84, EGCG 1.22 ± 0.03), and iNOS (LPS 40.45 ± 11.17, EGCG 10.24 ± 0.89). The GO function analysis identified that these differential genes involved 24 biological processes, 18 molecular functions, and 19 cellular component‐related processes. KEGG pathway enrichment analysis revealed that LPS significantly affects NF‐κB, TNF, and TLR signaling pathways. Western blotting revealed that EGCG diminished P‐IκB/IκB ratio by 75% and p‐p65/p65 by 50% compared to the LPS group. Finally, Arg‐1 and CD‐206 mRNA expression were determined by RT‐PCR, which was consistent with the RNA‐Seq result. These findings indicate that EGCG exerts an anti‐inflammatory effect by reducing NO and ROS production, suppressing TLR4 protein expression, and inhibiting IκB and p65 phosphorylation.
Spatial–temporal patterns of brain disconnectome in Alzheimer's disease
Authors: Liang Li , Zhou Pengzheng , Ye Chenfei , Yang Qi , Ma Ting 
Published: May 2023

AbstractMounting evidences have shown that progression of white matter hyperintensities (WMHs) with vascular origin might cause cognitive dysfunction symptoms through their effects on brain networks. However, the vulnerability of specific neural connection related to WMHs in Alzheimer's disease (AD) still remains unclear. In this study, we established an atlas‐guided computational framework based on brain disconnectome to assess the spatial–temporal patterns of WMH‐related structural disconnectivity within a longitudinal investigation. Alzheimer's Disease Neuroimaging Initiative (ADNI) database was adopted with 91, 90 and 44 subjects including in cognitive normal aging, stable and progressive mild cognitive impairment (MCI), respectively. The parcel‐wise disconnectome was computed by indirect mapping of individual WMHs onto population‐averaged tractography atlas. By performing chi‐square test, we discovered a spatial–temporal pattern of brain disconnectome along AD evolution. When applied such pattern as predictor, our models achieved highest mean accuracy of 0.82, mean sensitivity of 0.86, mean specificity of 0.82 and mean area under the receiver operating characteristic curve (AUC) of 0.91 for predicting conversion from MCI to dementia, which outperformed methods utilizing lesion volume as predictors. Our analysis suggests that brain WMH‐related structural disconnectome contributes to AD evolution mainly through attacking connections between: (1) parahippocampal gyrus and superior frontal gyrus, orbital gyrus, and lateral occipital cortex; and (2) hippocampus and cingulate gyrus, which are also vulnerable to Aβ and tau confirmed by other researches. All the results further indicate that a synergistic relationship exists between multiple contributors of AD as they attack similar brain connectivity at the prodromal stage of disease.
PCSK9 promotes T helper 1 and T helper 17 cell differentiation by activating the nuclear factor‐κB pathway in ankylosing spondylitis
Authors: Cai Jianfei , Jiang Yinghui , Chen Fucai , Wu Shubin , Ren Hongjun , Wang Pingping , Wang Jiayong , Liu Wei 
Published: May 2023

AbstractObjectiveOur previous study reveals that proprotein convertase subtilisin/kexin type 9 (PCSK9) is positively related to inflammatory markers, T helper (Th)‐17 cells, and treatment response in ankylosing spondylitis (AS) patients. Subsequently, this study aimed to explore the effect of PCSK9 on Th cell differentiation and its potential molecular mechanism in AS.MethodsSerum PCSK9 was determined by enzyme‐linked immunosorbent assay in 20 AS patients and 20 healthy controls (HCs). Then naïve CD4+ T cells were isolated from AS patients and infected with PCSK9 overexpression or knockdown adenovirus followed by polarization assay. Afterward, PMA (an NF‐κB activator) was administrated.ResultsPCSK9 was increased in AS patients compared to HCs (p < .001), and it was positively related to Th1 cells (p = .050) and Th17 cells (p = .039) in AS patients. PCSK9 overexpression increased the CD4+IFN‐γ+ cells (p < .05), CD4+IL‐17A+ cells (p < .01), IFN‐γ (p < .01), and IL‐17A (p < .01), while it exhibited no effect on CD4+IL‐4+cells or IL‐4 (both p > .05); its knockdown displayed the opposite function on them. Moreover, PCSK9 overexpression upregulated the p‐NF‐κB p65/NF‐κB p65 (p < .01), while it had no effect on p‐ERK/ERK or p‐JNK/JNK (both p > .05); its knockdown decreased p‐NF‐κB p65/NF‐κB p65 (p < .01) and p‐JNK/JNK (p < .05). Then, PMA upregulates p‐NF‐κB p65/NF‐κB p65 (p < .001) and increased CD4+IFN‐γ+ cells, CD4+IL‐17A+ cells, IFN‐γ, and IL‐17A (all p < .01), also it alleviated the effect of PCSK9 knockdown on NF‐κB inhibition and Th cell differentiation (all p < .01).ConclusionPCSK9 enhances Th1 and Th17 cell differentiation in an NF‐κB‐dependent manner in AS, while further validation is necessary.
Contaminated meal intake (Aeromonas hydrophila) does not elicit hormonal or immune modulation in bullfrogs (Lithobates catesbeianus)
Authors: Figueiredo Aymam Cobo de , Christie Monteiro Titon Stefanny , Lima Alan Siqueira , Floreste Felipe Rangel , Neto Patrício Getúlio Garcia , Gomes Fernando Ribeiro 
Published: May 2023

AbstractThe gastrointestinal tract (GIT) is colonized by resident microbiota but contact with foreign microbiota during feeding can impair GIT functions. During meal digestion, several vertebrates modulate the systemic immune function and immunoregulatory hormones concentration. However, in ectothermic animals, it is not known if this hormonal and immune modulation during the postprandial period is affected by the presence of pathogenic microbiota in the food. This study aimed to investigate the effects of contaminated meal ingestion on hormonal and innate immune responses in bullfrogs (Lithobates catesbeianus). Bullfrogs were divided into three treatments: fed three times with sterilized fish feed (control group), fed twice with sterilized fish feed and once with fish feed containing live bacteria (Aeromonas hydrophila, 109 UFC/mL), and fed three times with fish feed containing live bacteria. Blood and GIT tissues were collected 24 h after treatments to measure plasma and tissue corticosterone levels, NL ratio, and plasma bacterial killing ability. The ingestion of contaminated meal did not affect the hormonal and immune parameters. In conclusion, ingestion of contaminated food was not capable of intensifying the hypothalamic–pituitary–interrenal axis activation and the consequent hormonal and immune responses observed after feeding in bullfrogs. However, our results suggest that the ingestion of three contaminated meals tended to decrease stomach corticosterone levels (nonstatistically significant), possibly contributing to preventing the transmigration of the bacteria to organs outside the GIT.