bluuebunny/crossref_metadata_2025_split huggingface dataset:
An improved real-time detection algorithm based on frequency interpolation
Authors: Shi Heping , Yang Zikai , Shi Jin 
Published: July 2023

AbstractReal-time monitoring of heart rate (HR), i.e., extraction of heart rate variability (HRV), plays an important role in diagnosis and prevention of cardiovascular diseases. Compared with traditional contact monitoring devices, the use of continuous wave (CW) Doppler radar to monitor HRV does not require contact and is not sensitive to light and temperature, which makes it more and more popular. To monitor the HRV based on CW Doppler radar, the time window must be shortened to less than 5 s, which will lead to the spectrum leakage and degrade the measurement accuracy of HRV. To solve this problem, a custom CW Doppler radar has been developed in an integrated fashion on a single PCB, whose transmitting frequency and power of the radar are 24 GHz and 3 dBm, respectively. Furthermore, four frequency interpolation algorithms are introduced to compare their extraction accuracy. Experiments are performed on three subjects, and results show that the Quinn algorithm can obtain best HRV extraction results compared with other algorithms. Specially, the average HRV extraction error is 3.61% using the Quinn algorithm.
Expanding the range of the respiratory infectome in Australian feedlot cattle with and without respiratory disease using metatranscriptomics
Authors: Brito Barbara P. , Frost Melinda J. , Anantanawat Kay , Jaya Frederick , Batterham Tony , Djordjevic Steven P. , Chang Wei-Shan , Holmes Edward C. , Darling Aaron E. , Kirkland Peter D. 
Published: July 2023

Abstract
Background
Bovine respiratory disease (BRD) is one of the most common diseases in intensively managed cattle, often resulting in high morbidity and mortality. Although several pathogens have been isolated and extensively studied, the complete infectome of the respiratory complex consists of a more extensive range unrecognised species. Here, we used total RNA sequencing (i.e., metatranscriptomics) of nasal and nasopharyngeal swabs collected from animals with and without BRD from two cattle feedlots in Australia.

Results
A high abundance of bovine nidovirus, influenza D, bovine rhinitis A and bovine coronavirus was found in the samples. Additionally, we obtained the complete or near-complete genome of bovine rhinitis B, enterovirus E1, bovine viral diarrhea virus (sub-genotypes 1a and 1c) and bovine respiratory syncytial virus, and partial sequences of other viruses. A new species of paramyxovirus was also identified. Overall, the most abundant RNA virus, was the bovine nidovirus. Characterisation of bacterial species from the transcriptome revealed a high abundance and diversity of Mollicutes in BRD cases and unaffected control animals. Of the non-Mollicutes species, Histophilus somni was detected, whereas there was a low abundance of Mannheimia haemolytica.

Conclusion
This study highlights the use of untargeted sequencing approaches to study the unrecognised range of microorganisms present in healthy or diseased animals and the need to study previously uncultured viral species that may have an important role in cattle respiratory disease.
Determinants of neural tube defects among newborns in public referral hospitals in Eastern Ethiopia
Authors: Mohamed Fadumo Ahmed , Dheresa Merga , Raru Temam Bashir , yusuf Newas , Hassen Tahir Ahmed , Mehadi Ame , Wilfong Tara , Tukeni Kedir Negesso , Kure Mohammed Abdurke , Roba Kedir Teji 
Published: July 2023

Abstract
Background
Neural tube defects (NTDs) are serious brain and spine birth defects. Although NTDs are primarily pregnancy complications, such as abortion and stillbirth, they also contribute to under-five morbidity and mortality, as well as long-term disability and psychological impact. Despite these negative outcomes, the determinants of NTDs are not widely studied in Ethiopia, particularly in the country’s east. As a result, we sought to identify the risk factors for NTDs in neonates born in public referral hospitals in eastern Ethiopia.

Methods
A facility-based unmatched case-control study was carried out at Hiwot Fana Comprehensive Specialized Hospital and Sheik Hassen Yabare Jigjiga University Referral Hospital in Eastern Ethiopia. We included 59 cases identified in the selected facilities between September 10, 2021, and February 5, 2022, and 118 control neonates, with a case-to-control ratio of 1:2. Data were gathered through the use of interviewer-administered questionnaires and medical record review. To identify determinant factors of NTDs, a multivariable logistic regression model was used, which included all predictor variables from the bivariable analysis. The results were reported using an Adjusted Odds Ratio (AOR) with a 95% confidence interval. A p-value of < 0.05 was considered statistically significant.

Results
In total, 59 cases of NTDs were identified out of 2915 live birth total births registered in the two hospitals, making the incidence of NTDs 202.4/10,000 births. In the final model analysis, determinant factors such as gender of newborn [AOR = 2.97; 95%CI(1.27, 6.92)], having no history of antenatal care[AOR = 4.45;95%CI(1.30,15.20)], having a poor food consumption score (AOR = 3.38;95% CI;1.06,10.72), having history of monotonous diet consumption (AOR = 4.80; 95%CI: 1.09, 9.08; P = 0.038), and coffee consumption of three or more cups per day during pregnancy (AOR = 3.84:95% CI: 1.23, 11.97) were statistically associated with NTDs.

Conclusion
Modifiable and non-modifiable determinants were identified as major contributors of neural tube defect in this study. Early screening, dietary intervention counseling to increase consumption of a healthy diet, coffee consumption reduction, and pre-pregnancy supplementation programs should be developed to reduce NTDs in Ethiopia.
Prevalence and impact of comorbidities in hospitalized patients with COVID-19: a study from Saudi Arabia
Authors: alqahtani Saad M. A. , Al Saglan Naif S. , AlShehry Ali A. , Hawan Ali , A.Dafalla Badraltamam A. , Mohamed Nahid A. S. , Kadasah Ali S. , A.Qureshi Muhammad , Al-Shahrani Nourah A. , Alfaifi Abdulaziz S. Y. , Alqahtani Mohammed A. , Alshafa Fawwaz A. , Alsalem Ali A. , Al-Imamah Yahya A. , Abdullah Alahmari , Alzulfah Saad A. S. , Mahmoud Ibrahim M. A. , Abuelhassan Usama E. 
Published: July 2023

Abstract
Background
In this study, we aimed to address the prevalence of comorbidities and their impact on the outcomes of hospitalized COVID-19 patients admitted to a large tertiary Saudi Arabian hospital.

Methods
This is a retrospective study that included all adults with COVID‑19 admitted to a large tertiary Saudi Arabian hospital, between January 1, 2021, and September 30, 2022. The study outcomes were the prevalence of comorbidities among hospitalized COVID-19 patients and the effects of these comorbidities on all‑cause hospital mortality.

Results
A total of 1118 /1853 (60.3%) patients had one or more comorbidities. The most prevalent comorbidity was diabetes mellitus (48.5%), followed by hypertension (12.5%), and chronic renal disease (10.3%). Age (OR 3.032 (95% CI 0.006 – 0.029, p = 0.002), clinical status (8.194, 0.0350 – 0.709, p < 0.001), the presence (versus absence) of comorbidities (3.167, 0.042—0.233, p = 0.002), the number of comorbidities (2.972, 0.027 – 0.133, p = 0.003), and the 4C score (2.894, 0.010 – 0.054, p = 0.004), were independent significant predictors of mortality.

Conclusions
A total of 60.3% of hospitalized COVID-19 patients had one or more comorbidities, the most prevalent of which were diabetes mellitus, hypertension, and chronic renal disease. The presence and the number of comorbidities, but not the individual ones, together with age, clinical status at admission, and the 4C mortality score were significant independent predictors of mortality.
Sexual Fluidity: Implications for Population Research
Authors: Mittleman Joel 
Published: July 2023

AbstractFor the first time ever, national censuses have begun asking adults to report their sexual orientations. However, because such surveys provide only cross-sectional snapshots of populations, these data obscure one key complexity: that sexuality can be fluid, with sexual self-identification evolving over time. Drawing on unique, restricted-use data from the Population Assessment of Tobacco and Health, the current study documents the prevalence, correlates, and empirical consequences of sexual fluidity in the contemporary United States. Overall, about 1 in 11 American adults changed sexual identities over five annual surveys, including 6% of cisgender men, 11% of cisgender women, and 35% of gender minorities. Fluidity was particularly pronounced among young adults and among those who had ever identified as bisexual or “something else.” Despite the frequency and patterning of sexual fluidity, accounting for fluidity did little to change observed patterns of disadvantage on three measures of sexual minority well-being: mental health, financial insecurity, and substance use. Given these facts, I argue that demographic research should foreground the complexities inherent in quantifying sexuality, focusing less on how many people “are” a given sexual orientation and more on the social dynamics that continue to produce sexual minority disadvantage.
A Novel Two-step Adsorption–flotation Process Applied to the Efficient Extraction and Precise Separation of Rb+ and Cs+ from Lithium–extraction Tailings
Authors: Fang Dezhen , Lu Miao , Wang Yanping , Zhang Qiongyuan , Ma Liang , Li Kexin , Liu Haining , Zhang Huifang , Shi Guosheng , Wu Zhijian , Ye Xiushen 
Published: July 2023

Abstract
Rb+ and Cs+ are important components of lepidolite lithium–extraction tailings. However, efficient extraction and separation of Rb+ and Cs+ are difficult. We developed a two-step adsorption–flotation strategy for extraction and separation of Rb+ and Cs+. This new strategy enabled extraction of Rb+ and Cs+ from lithium–extraction tailings with efficiencies &gt;99% and the Cs+/Rb+ separation ratio was 20:1. These findings will shed light on the efficient extraction and precise separation of Rb+ and Cs+ in liquid minerals.
Case Report: Smoking as the risk factor of persistent STEMI after primary percutaneous coronary intervention: how it could be happen?
Authors: Pintaningrum Yusra , Yusuf Ricky Setiadi , Ramdani Baiq Hanida Aolia , Putri Shadiqa Rana , Wulandari Dwi Astuti 
Published: July 2023

Background Acute coronary syndrome (ACS) is a common disease. Smoking may increase the risk of ACS. The most advantageous therapy is percutaneous coronary intervention. This therapy may fail which is no-reflow phenomenon as the result. Total occlusion may increase the risk of no-reflow phenomenon which it could be worse with smoking as the habits. ST-elevation myocardial infarction (STEMI) may show in electrocardiogram (ECG). Case description A 37-year-old male came to the hospital with chest pain as the main complaint. ECG examination showed that there was wide anterior STEMI. Coronary angiography was then done and confirmed that there was total occlusion in left anterior descending artery. After two days hospitalization, the patient developed to cardiogenic shock and lead to acute decompensated heart failure. An ECG showed there was STEMI anterior after primary PCI. Discussion Many chemicals agent contain in cigarette smoking and it may induce the lipid oxidation which leads to plaque deposits. Plaque that deposits in coronary artery may rupture and make thrombus occlusion. This occlusion may partial or total, when there is total occlusion, STEMI was the result. Then, releasing the occlusion is needed for this situation ant PCI may be chosen as the therapy. Patient with wide ischemia may result the no-reflow phenomenon which may lead to heart failure and shock cardiogenic as the complication. Conclusion Smoking may induce ACS which leads to STEMI and may increase the failure of PCI therapy. No-reflow phenomenon is the evidence of miscarriage in therapy which it may increase because of smoking.
Synergistic effect of the combination of Chrysanthemum cinerariifolium (Trev.) and doxorubicin in inhibiting PI3K and Cyclin D in oral squamous cell carcinoma: in vitro study
Authors: Listiyana Anik , Rachmawati Yuanita Lely , Susianti Hani , Nurdiana Nurdiana , Sujuti Hidayat , Mutiah Roihatul , Endharti Agustina Tri 
Published: July 2023

Background: The most common type of lips and oral cavity cancer is oral squamous cell carcinoma (OSCC). Doxorubicin (DX) is commonly used as a chemotherapy drug, but its use is limited due to risk factors and drug resistance. Chrysanthemum cinerariifolium (Trev.) (CC) has potential as an anticancer agent. Combining the plant extract and chemotherapy drug might prevent OSCC proliferation by inhibiting PI3K and cyclin D protein. Therefore, the present study aimed to determine the synergistic effect of the combination of C. cinerariifolium (Trev.) and doxorubicin in inhibiting PI3K and Cyclin D protein. Methods: Human oral squamous carcinoma cell lines SCC-9 were used in this study. A cytotoxicity assay was performed to obtain the IC50 value of CC ethanol extract and DX on the SCC-9 cell line. Synergism evaluation of the combination CC and DX was analyzed using CompuSyn software. ELISA and the immunofluorescent assay were performed to determine the level of PI3K and cyclin D in the SCC-9 cell line after being treated with IC50 value of CC, IC50 value of DX and three combinations of CC and DX [7/8 IC50 CC + 1/8 IC50 DX (dose 1), 6/8 IC50 CC + 2/8 IC50 DX (dose 2), and 4/8 IC50 CC + 4/8 IC50 DX (dose 3). Results: CC stem ethanol extract and DX inhibited the proliferation of SCC-9 cell lines with the IC50 value of 133.4 µg/mL and 288.3 nM, respectively. The combination of CC and DX at dose 2 (6/8 IC50 CC + 2/8 IC50 DX) exhibited a high decrease in PI3K and cyclin D expression. Conclusions: The combination of C. cinerariifolium and doxorubicin synergistically declined OSCC proliferation by inhibiting PI3K and cyclin D expression.
Play and dance therapy: a review of theories, techniques and didactic methodologies for the motor recovery of children with disabilities
Authors: Valentini Manuela , Teloni Laura , Prokopiak Anna 
Published: July 2023

Background: This research is aimed at analysing the effectiveness of play activities and dance-therapy programmes in improving the psycho-physical condition and increasing the well-being of individuals with disabilities. Methods: Online search engines (e.g., EBSCOhost, PubMed, the American Journal of Dance) were consulted using parameters such as "physical disabilities" or "dance with music" or "childhood" and excluding protocols published before 2010. Eleven intervention protocols were analysed. These concerned play and dance-therapy courses targeted at disabled subjects under 14 years of age. Results: It was found that at the end of the play sessions, higher muscular activation and improvement of quality of communication exchanges with the family members was found. With regard to the dance-therapy protocols, improvement was reported in general coordination, balance ability and a decrease in maladaptive behaviour in children with disabilities. Conclusions: With the improvement in physical performance, an increase in the subjects' state of well-being is also noted, underlining a strong body-mind connection. For future studies, it is recommended that the number of participants in individual studies should be larger and to include a control group to make the results generalisable on a large scale.
The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence
Authors: Alshafie Walaa , Fotouhi Maryam , Ayoubi Riham , Shlaifer Irina , Southern Kathleen , McPherson Peter S. , Laflamme Carl ,   NeuroSGC/YCharOS/EDDU collaborative group
Published: July 2023

Charged multivesicular body protein 2B is a subunit of the endosomal sorting complex required for transport III (ESRCT-III), a complex implicated in the lysosomal degradation pathway and formation of multivesicular bodies. Mutations to the CHMP2B gene can result in abnormal protein aggregates in neurons and is therefore predicted to be associated in neurodegenerative diseases, including across the ALS-FTD spectrum. Through our standardized experimental protocol which compares read-outs in knockout cell lines and isogenic parental controls, this study aims to enhance the reproducibility of research on this target by characterizing eight commercial antibodies against charged multivesicular body protein 2b using Western Blot, immunoprecipitation, and immunofluorescence. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
Fractional microneedle radiofrequency - mechanism of action and assessment of safety, effectiveness in the treatment, and possible side effects based on a review of scientific literature
Authors: Nowak Aleksandra , Nowak Alicja , Bogusz Klaudia , Cywka Łucja , Baran Natalia , Bielak Adriana , Szwed Weronika , Maksymowicz Marcela , Machowiec Piotr 
Published: July 2023

Introduction and purpose:
For all skin types, micro-needle radiofrequency (FMR) can be a safe and effective aesthetic medicine procedure for the treatment of various dermatological and aesthetic conditions. The aim of the study is to present the mechanism of action and assess safety, treatment efficacy, and possible side effects on the basis of a review of the scientific literature.
Description of the state of knowledge:
FMR uses micro-needles to penetrate the skin to a depth of up to 4.5 mm and deliver radiofrequency current in a fractional manner, producing the controlled fractions of micro-injuries in the dermis, inducing neocollagenesis, neoelastinogenesis, and angiogenesis. FMR has a wide range of positive effects such as skin rejuvenation, skin tightening, facial and body scar remodeling, and hair growth through transdermal collagen induction.
Summary:
Increasingly, patients are looking for minimally invasive methods of skin tightening and fat remodeling. In response, Aesthetic Medicine has proposed a combination of two techniques, micro-needling and radiofrequency. As more and more studies are showing the effectiveness and efficiency of this procedure, also emphasizing its safety, minimal risk of serious side effects, and ease of use, micro-needle radiofrequency is being proposed as an attractive answer to people's desires for a less invasive method of skin rejuvenation. However, further research in this area is needed to optimize the parameters of the treatment to achieve the best results.
Prevention and treatment of tuberculosis before two great discoveries of the 20th century: the Bacillus Calmette-Guérin vaccine and streptomycin
Authors: Adamowicz Dominik , Łopuszyńska Inga , Zembala Julita , Stańczyk Justyna , Meliksetian Astrik , Wosińska Alicja , Pazik Dorota , Kosecka Katarzyna , Cieślik Aleksandra , Jargieło Anna 
Published: July 2023

Introduction and Purpose. Fundamental milestone in fight against tuberculosis is the invention of the effective vaccine, but before Anthony van Leeuwenhoek was the first to observe the bacteria, which opened the door to the first vaccinations in the 19th century. Medicine had battled against infectious diseases in different ways. Some methods can be perceived as a kind of primitive vaccines, which couldn’t, however, considerably affect a decrease in the epidemic range, due to their uncommon use and limited effectiveness. The aim of this paper is to analyze the history of tuberculosis and the role of Poland in the fight against it.
Materials and methods. PubMed database was used for review of the literature. The following phrases were searched in English: “tuberculosis”, “vaccination”, “BCG vaccine”, “sanatorium”.
Description of the state of knowledge. Poland played an important role in the fight against tuberculosis. It was in the laboratory of Polish pharmacist, where Koch discovered its etiological factor. In the territory of Poland there are prototypes of modern sanatoria for tuberculosis patients. Article also presents old and modern methods of tuberculosis treatment, as the use of specific climatic conditions, which resulted in developing health-resort treatment. A breakthrough in the methods used was the discovery of streptomycin in the middle of the 20th century, which led to the development of pharmacological treatment, nowadays involving the administration of proper combinations of drugs.
Summary. The increasingly strong anti-vaccine movement is slowly destroying what people have been fighting for the centuries. It is worth recalling how we reached milestones in this fight and why immunization is so important for all societies.
Alcohol in minors - a harmless fad or a serious problem?
Authors: Świątek Gabriela , Tkaczyk Rafał , Sosnowska Weronika , Tomkiewicz Maria , Taracha Kalina , Tomczyk Jakub , Tomkiewicz Maciej , Brzozowska Aleksandra , Śwital Magdalena , Tokarski Michał 
Published: July 2023

Introduction and purpose: Alcohol has been known for centuries. In recent years, awareness of the effects of its consumption has been growing and acceptance of the drink has been declining. Despite this, there is an increase in young alcohol consumption and a decrease in the age of alcohol initiation. In this work, the problem of alcohol consumption by minors will be reviewed based on the evaluation of hospitalizations in the Clinical Department of Toxicology and Cardiology of the Stefan Cardinal Wyszynski Regional Hospital in Lublin in 2022. The aim of the study is to draw attention to this important, although often downplayed, problem.
Material and method: The study was retrospective in nature. It used an analysis of the records of 120 intoxications in people under the age of 18, from which a group of alcohol-poisoned people was distinguished, consisting of 37 cases.
Results: Hospitalization of 7 patients aged 13 to 18 years was reported due to alcohol intoxication. Of these, 22 (59.5%) female and 15 (40.5%) male patients were singled out. Most were urban residents 29 (78.4%). Isolated alcohol intoxication was the cause of 18 hospitalizations(48.6%). The remaining cases were accompanied by drug intoxication 11 hospitalizations (29.7%) and drug intoxication 8 hospitalizations (21.6%). All analyzed intoxications were intentional, of which 9 (24.3%) were suicide attempts. In 14 cases (37.9%), patients were burdened with mental illness, the most common of which was depressive disorders. There were no deaths in the study group.
Conclusions: Alcohol should be forbidden and unacceptable in the family and young people's environment. Parents and young people should be made aware of the harmful effects, psychological, social and health consequences of its consumption.