bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of TNFRSF14 and LIGHT in biliary epithelial cells of patients with primary sclerosing cholangitis
Authors: Kanai Sachiko , Fujiwara Hiroaki , Mizuno Suguru , Kishikawa Takahiro , Nakatsuka Takuma , Hamada Tsuyoshi , Tanaka Mariko , Arita Junichi , Nakai Yousuke , Isayama Hiroyuki , Kasuga Masato , Tateishi Ryosuke , Tateishi Keisuke , Ushiku Tetsuo , Hasegawa Kiyoshi , Koike Kazuhiko , Fujishiro Mitsuhiro 
Published: January 2023

Abstract

Background and aims: There is a lack of biliary epithelial molecular markers for primary sclerosing cholangitis (PSC). We analyzed candidates from disease susceptibility genes identified in recent genome-wide association studies.
Methods: Expression was quantified using immunohistochemistry in biliary epithelia in liver biopsy samples from patients with PSC (N = 45) and controls (N = 12). Samples from patients with primary biliary cholangitis (PBC) were used as disease controls (N = 20).
Results: The levels of hepatic expression of ATXN2, HHEX, PRDX5, MST1, and TNFRSF14 were significantly altered in the PSC group. We focused on the immune-related receptor, TNFRSF14. Immunohistochemistry revealed that TNFRSF14 positivity was significantly higher in biliary epithelia in the PSC group (96 %) than in the control (42 %) and PBC (55 %) groups. High expression of TNFRSF14 was observed only in patients with PSC. Moreover, the expression of LIGHT, which encodes a TNFRSF14-activating ligand, was increased in PSC liver. Immunohistochemistry showed that high expression of LIGHT was more common in PSC biliary epithelia (53 %) than in the PBC (15 %) or control (0 %) groups; moreover, it was positively associated with fibrotic progression.
Conclusions: TNFRSF14 and LIGHT are attractive candidate markers for PSC.
Fluorescein-Guided Surgery for High-Grade Glioma Resection: A five-year long retrospective study in our institute
Authors: Xi Chen , Jinli Sun , Yan Chen , Huijuan Li , Zhongjie Shi , zhangyu Li , Jianyao Mao , Liwei Zhou , Yukui Li , Sifang Chen , Guowei Tan 
Published: January 2023

Abstract
Objective: To investigate the outcomes and adverse events of glioma patients received surgery with or without fluorescein sodium guidance.
Methods: A total of 136 patients who visited our hospital and underwent surgery from July 2017 to June 2022 were retrospectively analyzed. 75 cases in the fluorescein group, while 61 cases in the non-fluorescein group. Baseline characteristics, intraoperative blood loss, duration of operation and tumor mass resection were documented, adverse events were also recorded.
Results: The duration of operation was significantly shorter in the fluorescein group than in the non-fluorescein group (P = 0.008), especially for patients with tumors in the frontal and occipital lobes (frontal, P = 0.036; occipital, P = 0.041). More critically, the rate of gross total resection (GTR) was significantly higher in the fluorescein group than in the non-fluorescein group (42.67% vs. 21.31%, P = 0.005), particularly for patients with tumors located in the temporal lobe, occipital lobe, and deep supratentorial area (temporal, 48.15% vs. 13.04%, P = 0.002; occipital, 53.84% vs. 0.00%, P = 0.026; deep supratentorial region, 15.00% vs. 5.88%, P = 0.016). however, there was no statistically significant difference in intraoperative blood loss (P = 0.094) or incidence of complications (6.67% vs. 4.92%, P = 0.668) between the two groups.
Conclusions: Fluorescein-guided resection of high-grade gliomas using a dedicated filter on the operating microscope is feasible, safe, convenient and significantly increases the GTR rate; this is especially advantageous in patients with tumors located in non-verbal and motor areas such as the temporal and occipital lobes and deep supratentorial areas.
Green Supplier Selection in the Automobile Industry: A Comprehensive Framework for Multi-Criteria Decision Analysis
Authors: GHOSH SUDIPTA , Bhowmik Chiranjib , Sinha Sudipta , Mandal Madhab Chandra , Ray Amitava 
Published: January 2023

Abstract
This research proposes a comprehensive framework to evaluate green suppliers. A real-world green supplier selection (GSS) problem is considered in which three India-based automobile manufacturing organizations, namely Supplier 1, Supplier 2, and Supplier 3 are selected for the evaluation. Initially, fourteen important criteria are extracted from literature and experts’ suggestion, which encompasses both the environmental, economic, and operational aspects of sustainability. Firstly, data is collected through the development of a questionnaire, formation of an expert committee, and carrying out interviews with the experts. Secondly, an integrated multi-criteria decision-making (MCDM)-multivariate approach is proposed in which the item analysis is used to examine the internal consistency of data, the principal component analysis (PCA) is used to calculate the weights of the parameters. Finally; the simple additive weighting (SAW) method is used to determine the performance scores of the case organizations for ranking and benchmarking purpose. The result reveals that Supplier 3 is the benchmark supplier as it gains the highest performance score. The result also recommends that collaboration with suppliers for green purchasing is the most influential parameter for GSS. This research may assist policymakers to strategically select the optimum green supplier for sustainability.
Evaluation of lung adverse events with nivolumab using the spontaneous reporting system in Japan: a pharmacovigilance study
Authors: Kanbayashi Yuko , Shimizu Tadashi , Kojima Asuka , Anzai Miku , Kawai Rika , Uchida Mayako 
Published: January 2023

Abstract
Purpose: Nivolumab has been widely used in patients since its launch, but detailed information on lung-specific adverse events (AEs) from post-marketing monitoring has not been reported. We therefore conducted this study to examine times to onset, incidence rates, and outcomes of nivolumab-induced lung AEs in patients with cancer, using the Japanese Adverse Drug Event Report database.
Methods: We analysed data for the period between April 2004 and March 2021. Data on lung AEs were extracted, and relative risks of AEs were estimated using the reporting odds ratio.
Results: We analysed 5,273,115 reports and identified 18,721 reports of AEs caused by nivolumab. Of these, 3,084 lung AEses were reportedly associated with nivolumab. Signals were detected for nine lung AEs (expressed using terminology preferred by the Medical Dictionary for Regulatory Activities): interstitial lung disease; pneumonitis; lung disorder; organising pneumonia; pleural effusion; pneumonia aspiration; pneumonia bacterial; radiation pneumonitis; and infectious pleural effusion. Among these, interstitial lung disease was the most frequently reported (68.7%) and included some fatal cases. A histogram of median times to onset showed AEs occurring from 34 to 79 days after the first dose, but some cases occurred even more than one year after starting administration.
Conclusion: We focused on lung AEs caused by nivolumab as post-marketing AEs. Some cases could potentially involve serious outcomes, particularly in interstitial lung disease. Patients should be monitored for signs of the development of these AEs not only at the start of administration, but also over an extended period after starting nivolumab administration.
ELFN1-AS1 Promotes GDF15-Mediated Immune Escape of Colorectal Cancer from NK cells by Facilitating GCN5 and SND1 Association
Authors: Han Bin , He Jinsong , Chen Qing , Yuan Min , Zeng Xi , Li Yuanting , Zeng Yan , He Meibo , Zhou Qilin , Feng Dan , Ma Daiyuan 
Published: January 2023

Abstract
The ability of colorectal cancer (CRC) cells to escape from natural killer (NK) cell immune surveillance leads to anti-tumor treatment failure. The long non-coding RNA (lncRNA) ELFN1-AS1 is aberrantly expressed in multiple tumors suggesting a role as an oncogene in cancer development. However, whether ELFN1-AS1 regulates immune surveillance in CRC is unclear. Here, we determined that ELFN1-AS1 enhanced the ability of CRC cells to escape from NK cell surveillance in vitro and in vivo. In addition, we confirmed that ELFN1-AS1 in CRC cells attenuated the activity of NK cell by down-regulating NKG2D and GZMB via the GDF15/JNK pathway. Furthermore, mechanistic investigations demonstrated that ELFN1-AS1 enhanced the interaction between the GCN5 and SND1 protein and this influenced H3k9ac enrichment at the GDF15 promotor to stimulate GDF15 production in CRC cells. Taken together, our findings indicate that ELFN1-AS1 in CRC cells suppresses NK cell cytotoxicity and ELFN1-AS1 is a potential therapeutic target for CRC.
Spatial Variation in groundwater quality and Health Risk Assessment for Fluoride and Nitrate in Chhotanagpur Plateau, India
Authors: Sinha Heena , Rai Suresh Chand , Kumar Sudhir 
Published: January 2023

Abstract
The evaluation of groundwater quality is extremely important to assess the risk to human health. This study deals with the spatial variation in physico-chemical parameters of groundwater for drinking purposes and human health risk assessment concerning fluoride and nitrate. GIS techniques have been used to determine and delimit zones of pollution. Samples were collected in the post-monsoon season (November 2020) and analyzed for physico-chemical parameters such as pH, TDS, conductivity, cations, and anions. For drinking water quality assessment, analyzed parameters were compared with WHO standards, and Water Quality Index (WQI) was used. Results reveal that the majority of the samples come within the desired limit suggested by WHO. However, in a few samples, EC, TDS, TH, chloride, sulphate, and calcium are higher than the desirable limit, whereas fluoride and nitrate are beyond the maximum permissible limit in some of the samples. To assess health risk, the Hazard quotient (HQ) and total hazard index (THI) were computed. The results indicate that the total non-carcinogenic risk for children, male and female ranges from 0.01 to 7.46 for males, 0.009 to 7.055, and 0.01 to 7.34 for children respectively. Furthermore, 84%, 78%, and 82% of the samples are greater than the recommended limit of THI > 1 for males, females, and children respectively, suggesting detrimental impacts on the health of the residents. Knowledge of spatial variation and anomalous concentration is vital for groundwater management as well as health risk assessment. The findings of this study will be helpful to government officials, policy planners, NGOs, and local communities.
Clinical Outcomes in Ventilator-Associated Pneumonia Patients with and without Chronic Obstructive Pulmonary Disease: A Retrospective Observational Study
Authors: Lu Caiden Taowei , Hung Ming-Szu , Lin Yi-Tsung , Fang Tien-Pei 
Published: January 2023

Abstract
Background
Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide. Critically ill patients with COPD exacerbations may require invasive mechanical ventilation (IMV). Ventilator-associated pneumonia (VAP) commonly occurs in the intensive care unit (ICU) and is usually associated with high mortality. Current studies on the relationship between COPD and VAP are limited. This work compares the etiology and clinical outcomes of VAP between patients with and without COPD in Taiwan.
Methods
This retrospective observational study was conducted at the Chiayi Chang Gung Memorial Hospital. Patients with VAP were enrolled between January 2015 and December 2019. The COPD diagnosis was based on post-bronchodilator pulmonary function tests. We compared the bacterial etiology, ICU and hospital stay length, IMV duration, and mortality.
Results
A total of 175 patients with VAP were enrolled, 44% of whom presented pre-existing COPD. The disease severity on the day of admission was similar in both groups. Microorganisms were identified in 83 (47%) patients, with Pseudomonas aeruginosa, Acinetobacter spp., and Stenotrophomonas maltophilia being the most common pathogens. Acinetobacter spp. were more frequently isolated from patients with COPD than from those without. The proportion of multi-drug resistant isolates showed no significant differences between groups. Most patients underwent antibiotic treatment before VAP onset. The length of ICU and hospital stays and IMV duration after VAP onset were similar between groups, as well as ICU mortality and 30-, 60-, and 90-day mortalities.
Conclusions
Our study revealed that COPD was not associated with worse clinical outcomes in patients with VAP. Patients with VAP and concomitant COPD were more susceptible to Acinetobacter spp. than those without COPD. Our results may provide a rationale for empirical antibiotic coverage in this group.
Isolation and identification of Lacticaseibacillus rhamnosus YT and the antibacterial characteristics of its cell free supernatant and cells
Authors: Guan Chengran , Su Jianbo , Li Feng , Zhang Wenjuan , Chen Dawei , Chen Xia , Huang Yujun , Zhang Chenchen , Gu Ruixia 
Published: January 2023

Abstract
Lactic acid bacteria (LAB) which produce diverse imperative antimicrobial metabolites have an immense number of applications in food industry. Here, human-derived strain YT was isolated due to its cell free supernatant (CFS-YT) and cells (Cs-YT) respectively performed obvious inhibitory ring to Gram positive and negative spoilage bacteria. Strain YT was identified as Lacticaseibacillus rhamnosus YT by 16s rDNA sequence and morphology. The antibacterial activity of CFS-YT was demonstrated to be growth-dependent, pH sensitive, broad thermostable and proteases insensitive. Cs-YT displayed a broad antibacterial spectrum with the action mode of bacteriostatic. The antibacterial activity of Cs-YT was due to substances located at the cell surface which was sensitive to heat, stable at broad pH gradients and sensitive to specific proteases. These data suggested that L. rhamnosus YT could be used as alternative antimicrobial agents against foodborne spoilage bacteria in food industry.
A Case Study on the Spread of Fake News by Social Media in the COVID-19 Era in Bangladesh
Authors: Hossain Imran , Haque AKM Mahmudul 
Published: January 2023

Abstract
Fake news has negative impacts on society. It can sometimes cause the death of a person. In Joypurhat, Bangladesh, a garment worker has committed suicide because of fake news. At midnight, a passenger on a bus in Bangladesh's Naogaon district believed Corona had affected his mother. However, after testing, his Corona report came back negative. So, this incident has also happened based on fake news. With the internet, social media have become the most acclaimed tool for freedom of speech, democracy, truth, and as a source of infotainment. In this study, the researchers have attempted to find out whether social media is informing or misinforming the public with regard to the COVID-19 pandemic, adopting the qualitative method of study. The findings of this study will aid in determining whether social media is disseminating accurate or incorrect information to the public about the COVID-19 outbreak. The findings show that social media generates approximately 100 COVID-19-related fake news stories over the course of 150 days, from March 1 to July 30, 2020. In this research, the results also show that 64.6% of respondents get COVID-19 related information through social media. In this aspect, Facebook plays an important role. About 53.0% of social media users acclaimed that they get COVID-19 related fake information through Facebook. Facebook spreads 63.0% of fake news, and it is the quickest way to spread any fake news in a short period of time. Most of the fake news is a combination of text and video (49.0%). So, social media is a large platform for getting COVID-19 related information. Fake news and rumors are new words related to COVID-19. This study reveals that 87.3% of respondents are familiar with these words, whereas 12.7% were not familiar with them before the COVID-19 era. About 47.6% of respondents never try to verify any rumors or information related to COVID-19. However, there is less research analyzing the circulation of false and evidence-based information during health emergencies. Thus, the present study aims at shedding new light on the type of fake news that circulated on social media around the COVID-19 outbreak in order to analyze how false information was shared.
Effect of (4R, 5S) 4-hydroxy-7-angeloyloxycarvatoneacetone from Blumea axillaris (Lam.) DC. plant against An. stephensi and Cx. quinquefasciatus vector mosquitoes (Diptera: Culicidae) and expression of different functional genes
Authors: Hillary V. Edwin , Sivanandhan S. , Ceasar S. Antony , Ayyavoo K. , Tamilselvan C. , Kumar M. Ravi , shirotta Osamu , Balakrishna K. 
Published: January 2023

Abstract
The present study was conducted to evaluate the mosquitocidal efficiency using a compound isolated from Blumea axillaris (Lam.) DC. against Culex quinquefasciatus and Anopheles stephensi. The compound isolated from leaves of B. axillaris is (4R, 5S) 4-hydroxy-7-angeloyloxycarvatoneacetone. This isolated compound was exposed to eggs and larvae of An. stephensi and Cx. quinquefasciatus and with different concentrations of 0.5, 1.0, 1.5, and 2.0 ppm. Primarily, the hexane extract of an isolated compound exhibited promising larvicidal activities of LC50 and LC90 values of 155.811 and 566.763 ppm against An. stephensi and 77.215 and 99.653 against Cx. quinquefasciatus at 24 hours. Secondly, the compound isolated from B. axillaris at 2-ppm concentration exhibited LC50 and LC90 values of 0.85 and 3.59 and 1.19 and 3.678 ppm against Cx. quinquefasciatus and An. stephensi larvae, respectively. Furthermore, histopathological studies revealed serious damage to the larval midgut cells in both Cx. quinquefasciatus and An. stephensi (treated with compound). Additionally, it showed 81.0% and 84.2% ovicidal activity against both Cx. quinquefasciatus and An. stephensi eggs at 120 h post-treatment. In addition, we also analyzed the influence of isolated compound on the expression of different functional genes of An. stephensi and Cx. quinquefasciatus namely (1) White (Wh) gene, which is responsible for eye pigmentation; (2) the Glutathione transferase (GST) gene that helps for survival when exposed to any insecticides or pesticides; (3) Cytochrome P450 monooxygenase (CYP450) gene that enhances resistance in mosquitoes to digest pesticides and (4) Esterase (Est) gene, which helps in resistance to the variety of insecticides. These overall studies and results clearly suggest that compounds from B. axillaris could act as good mosquitocidal agents against both Cx. quinquefasciatus and An. stephensi and the compound were also first time reported as mosquitocidal activity.
Geographical Disparities of uncertainty stress and life stress among university students in China
Authors: Yang Tingzhong , Sihui Peng , Wang Huihui , Oliffe John L. 
Published: January 2023

Abstract
Background
Ecological models have emphasized that mental stress both is an individual phenomenon and an environmental outcome. However, many mental stress studies focused primarily on individual variables. Few studies have examined the geographical disparities in mental stress, with no comparable studies implemented on this issue with regard to different type of mental stress. This study investigates geographical variations in overall stress, uncertainty stress and life stress among university students in China.
Methods
Respondents comprised 11,954 students from 50 universities and 31 provinces in Mainland China. Respondents completed the extended version of the Global Health Professional Student Survey (GHPSS) on Tobacco Control in China. The survey included additional health, mental stress, and behavioral items along with regional variables which were retrieved from the National Bureau of Statistics database. Both unadjusted and adjusted methods were used in the logistic regression analysis.
Results
Uncertainty stress and life stress prevalence were 19.6% (95% CI: 15.9%, 23.3%) and 8.6% (95% CI: 7.2%, 10.7%) respectively, while overall stress prevalence was 37.2%, 95% CI: 24.7%, 49.7%). All stresses varied widely across the 31 provinces. Random parameters of 0.3971 for life stress, 0.2593 for uncertainty stress, and 0.1449 for overall stress were statistically significantly at the 0.01 level, in terms of their geographical distribution, high life stress was concentrated in central provinces, high uncertainty stress mainly in both central and western provinces, while provinces displaying higher overall stress tended to display a north-south distribution. Multilevel logistic regression showed province level per capital disposable income of households partly contributed to uncertainty stress (OR: 0.52) and life stress (OR: 0.59).
Conclusions
This study maps the geographical distribution of uncertainty stress, life stress, and overall stress in China. These findings underscore the importance of environmental factors affecting stress levels among university students.
An in-Silico approach to evaluate the binding efficacy and stability profile of MWCNT entangled rutin for breast cancer treatment
Authors: Bhusan Kirthi , Ammunje Damodar Nayak , Kunjiappan Selvaraj , Jyaraman Anbu , Devi Manisha , Pavadai Parasuraman 
Published: January 2023

Abstract
Cancer is one of the most devastating diseases and ranks second in a total number of deaths caused globally. In recent years there is a steady increase in breast cancer (BC) incidence due to several etiological factors. Due to indiscriminate drug delivery and the lack of target specificity, current cancer therapies can cause life-threatening side effects. The present research emphasises targeting the rutin-loaded onto carbon nanotubes (CNTs) for breast cancer treatment. Intermolecular interaction studies between rutin (PubChem CID 5280805) and the target protein folate receptor (PDB ID 4LRH) via Autodock Vina programme and PyRx tool was carried out, and the docking score was found to be -8.7 Kcal mol− 1. In comparison, that of the standard chemotherapeutic drug 5-Fluorouracil was − 5.9 Kcal mol− 1. Molecular dynamic studies were performed via Desmond for 100ns. The root-mean-square deviation (RMSD) value of the ligand remained stable, root-mean-square fluctuation (RMSF) values have been observed to be stable throughout the simulation time. Based on these promising results, rutin-loaded CNTs can be further evaluated for their efficacy against breast cancer preclinically.
Active poly (lactic acid) films with rice straw aqueous extracts for meat preservation purposes
Authors: Freitas Pedro A. V. , González-Martínez Consuelo , Chiralt Amparo 
Published: January 2023

Abstract
Aqueous extracts from rice straw, using ultrasound-assisted reflux heating extraction (USHT) and Subcritical Water Extraction (SWE), under two process conditions (160°C, 7 bars; and 180°C, 11 bars), were obtained and characterised as to their phenolic content and antioxidant and antimicrobial capacities. These extracts were incorporated (6% wt.) into PLA films by melt blending and compression moulding that were characterised as to their structural and functional properties and their capacity to preserve fresh pork meat, as vacuum thermo-sealed bags, throughout 16 days of cold storage. The extracts slightly reduced the extensibility, resistance to break and water barrier capacity of the PLA films but enhanced their oxygen barrier capacity and the UV light-blocking effect. The films with RS extracts were effective at preserving meat quality parameters, inhibiting microbial growth, meat oxidation, discolouration, and weight loss, thus extending the meat shelf life. The SWE extract obtained at 180°C was the most effective at obtaining active films for meat preservation, exhibiting the greatest antioxidant and antibacterial activity. Therefore, the green SWE technique is useful for obtaining active extracts from RS, allowing for its valorisation in the development of biodegradable active materials useful to extend the food shelf life.