bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams
Authors: Jurado-Campos Alejandro , Soria-Meneses Pedro Javier , Arenas-Moreira María , Alonso-Moreno Carlos , Rodríguez-Robledo Virginia , Soler Ana Josefa , Garde José Julián , del Rocío Fernández-Santos María 
Published: August 2023

AbstractBackgroundArtificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport.ResultsWith the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 106spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe2+/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.ConclusionsThe storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.Graphical Abstract
Lysin (K)-specific demethylase 1 inhibition enhances proteasome inhibitor response and overcomes drug resistance in multiple myeloma
Authors: Bandini Cecilia , Mereu Elisabetta , Paradzik Tina , Labrador Maria , Maccagno Monica , Cumerlato Michela , Oreglia Federico , Prever Lorenzo , Manicardi Veronica , Taiana Elisa , Ronchetti Domenica , D’Agostino Mattia , Gay Francesca , Larocca Alessandra , Besse Lenka , Merlo Giorgio Roberto , Hirsch Emilio , Ciarrocchi Alessia , Inghirami Giorgio , Neri Antonino , Piva Roberto 
Published: August 2023

Abstract
Background
Multiple myeloma (MM) is an incurable plasma cell malignancy, accounting for approximately 1% of all cancers. Despite recent advances in the treatment of MM, due to the introduction of proteasome inhibitors (PIs) such as bortezomib (BTZ) and carfilzomib (CFZ), relapses and disease progression remain common. Therefore, a major challenge is the development of novel therapeutic approaches to overcome drug resistance, improve patient outcomes, and broaden PIs applicability to other pathologies.

Methods
We performed genetic and drug screens to identify new synthetic lethal partners to PIs, and validated candidates in PI-sensitive and -resistant MM cells. We also tested best synthetic lethal interactions in other B-cell malignancies, such as mantle cell, Burkitt’s and diffuse large B-cell lymphomas. We evaluated the toxicity of combination treatments in normal peripheral blood mononuclear cells (PBMCs) and bone marrow stromal cells (BMSCs). We confirmed the combo treatment’ synergistic effects ex vivo in primary CD138+ cells from MM patients, and in different MM xenograft models. We exploited RNA-sequencing and Reverse-Phase Protein Arrays (RPPA) to investigate the molecular mechanisms of the synergy.

Results
We identified lysine (K)-specific demethylase 1 (LSD1) as a top candidate whose inhibition can synergize with CFZ treatment. LSD1 silencing enhanced CFZ sensitivity in both PI-resistant and -sensitive MM cells, resulting in increased tumor cell death. Several LSD1 inhibitors (SP2509, SP2577, and CC-90011) triggered synergistic cytotoxicity in combination with different PIs in MM and other B-cell neoplasms. CFZ/SP2509 treatment exhibited a favorable cytotoxicity profile toward PBMCs and BMSCs. We confirmed the clinical potential of LSD1-proteasome inhibition in primary CD138+ cells of MM patients, and in MM xenograft models, leading to the inhibition of tumor progression. DNA damage response (DDR) and proliferation machinery were the most affected pathways by CFZ/SP2509 combo treatment, responsible for the anti-tumoral effects.

Conclusions
The present study preclinically demonstrated that LSD1 inhibition could provide a valuable strategy to enhance PI sensitivity and overcome drug resistance in MM patients and that this combination might be exploited for the treatment of other B-cell malignancies, thus extending the therapeutic impact of the project.
Reimaging Earth. Architecture and the critical and speculative uses of geovisualization
Authors: Barrio Roi Salgueiro 
Published: August 2023

AbstractThis article addresses the debates surrounding the design uses of satellite-based geovisualizations. It asks, in particular, how geovisualizations can support new design politics towards global urbanization. The initial section of the article presents a series of theoretical critiques of geovisualization. These stress that satellite-based imagery represents a disembodied, detached form of vision that hides under a totalizing image the actual, variegated conditions and struggles happening on the ground. As a result, geovisualization is considered as an instrument that neutralizes the critical dimension of design. The article counters this critique through an analysis of two cartographic and design practices which heavily rely on satellite data. The first one is the work of Neil Brenner’s Urban Theory Lab (UTL), which uses cartography as an analytical and critical tool aimed at unpacking the sociospatial dimensions of planetary urbanization. The second case is Joyce Hsiang and Bimal Mendis’ speculative, cartographic project City of Seven Billion. My argument is that this project partially builds upon the UTL’s work, but it substitutes the latter’s analytical orientation for a projective one to investigate how architectural design can operate in the context of planetary urbanization. By analysing these two works, the article concludes that both support a design politics oriented to recognizing and then defining the questions and scales upon which architecture can intervene today.
How sociocultural factors mediate washback effect: a study of the Hong Kong Diploma of Secondary Education Examination English Language Paper 4
Authors: Wong Katagiri Nozomu 
Published: August 2023

AbstractThe study explores the unaddressed washback on the English-speaking test of the Hong Kong Diploma of Secondary Education Examination (HKDSE-English Language Paper 4). The HKDSE is a university entrance exam in Hong Kong which includes a group discussion speaking test known as English Language Paper 4. To identify ongoing washback effects, including sociocultural perspectives, the study analyses ongoing student washback of test-specific and non-specific preparation and how the mediating factors affect these preparations. One-hundred and ninety-nine secondary 4th to 6th grade students provided the questionnaire data in two rounds. Results from exploratory factor analysis indicate six major types of test preparation, which are categorised as two test specific, three non-specific, and one integrated type of preparation. Mediating factors comprise five categories of learner characteristics and stakeholders in and out of school. The cluster analyses identified four students’ groups intertwined with the involvement of washback and the mediating factors. A strong relationship between extrinsic factors and explicit learning and intrinsic factors and implicit learning was found. The study concludes that students’ washback is affected by mediating factors aside from the test; learning method choice by students (e.g. learning from entertainment content) has a strong relationship with specific mediating factors (e.g. interest in English).
Extraction, purification and antioxidant activity of Juglans regia shell polysaccharide
Authors: Zhou Shiyang , Huang Gangliang 
Published: August 2023

AbstractIn recent years, the biological activity of plant polysaccharides has attracted more and more attention. Juglans regia was one of the four-dry fruits in the world, it has very high value in nutrition and health care value was very high. To better develop the nutritional and health effects of the J. regia shell polysaccharide, J. regia shell was used as raw material to prepare polysaccharide by the water extraction and alcohol precipitation method. The molecular weight, monosaccharide components, molecular structure characteristics and antioxidant activities of its derivatives were studied. Then, a series of meaningful data information for the study of the J. regia shell polysaccharide and its derivatives were obtained. The molecular weight of the polysaccharide was 9.45 × 104 Da after the separation and purification by DEAE-52 and Sephadex G-100. The polysaccharides contained rhamnose, arabinose, galactose, glucose, xylose and galacturonic acid, and the molar ratio of each monosaccharide component was 6.7%: 16.5%: 28.3%: 11.2%: 12.5%: 24.8%. The molecular structure of J. regia shell polysaccharide contains β-glucoside bond, its structure may be → 4)-α-D-Galp-(1 → 4)-β-D-GalAp-(1 → 4)-α-D-Xylp-(1 → glycosidic bond). The study of antioxidant activity, J. regia shell polysaccharide and its derivatives showed good biological activities, especially the phosphorylated J. regia shell polysaccharide. The study of J. regia shell polysaccharide has important practical significance for improving the quality and efficiency of the J. regia industry chain.
Graphical Abstract
Moderate evidence exists for four microRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients: conclusion of a systematic review with best‐evidence synthesis
Authors: Schmid Tristan , Wegener Florian , Hotfiel Thilo , Hoppe Matthias W. 
Published: August 2023

AbstractPurposeThe aim of this systematic review was to investigate tendon‐specific microRNAs (miRNAs) as biomarkers for the detection of tendinopathies or degenerative tendon ruptures. Also, their regulatory mechanisms within the tendon pathophysiology were summarized.MethodsA systematic literature research was performed using the PRISMA guidelines. The search was conducted in the Pubmed database. The SIGN checklist was used to assess the study quality of the included original studies. To determine the evidence and direction of the miRNA expression rates, a best‐evidence synthesis was carried out, whereby only studies with at least a borderline methodological quality were considered for validity purposes.ResultsThree thousand three hundred seventy studies were reviewed from which 22 fulfilled the inclusion criteria. Moderate evidence was found for miR‐140‐3p and miR‐425‐5p as potential biomarkers for tendinopathies as well as for miR‐25‐3p, miR‐29a‐3p, miR‐140‐3p, and miR‐425‐5p for the detection of degenerative tendon ruptures. This evidence applies to tendons at the upper extremity in elderly patients. All miRNAs were associated with inflammatory cytokines as interleukin‐6 or interleukin‐1ß and tumor necrosis factor alpha.ConclusionsModerate evidence exists for four miRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients. The identified miRNAs are associated with inflammatory processes.
Application of first-generation high- and low-dose drug-coated balloons to the femoropopliteal artery disease: a sub-analysis of the POPCORN registry
Authors: Fujihara Masahiko , Takahara Mitsuyoshi , Soga Yoshimitsu , Iida Osamu , Kawasaki Daizo , Tomoi Yusuke , Tsubakimoto Yoshinori , Ogata Kenji , Karashima Eiji , Kato Taku , Kobayashi Yohei , Kaneko Nobuhito , Sasaki Shinya , Ichihashi Kei 
Published: August 2023

Abstract
Background
Drug-coated balloons (DCBs) have significantly changed endovascular therapy (EVT) for femoropopliteal artery (FPA) disease, in terms of the expansion of indications for EVT for symptomatic lower extremity arterial disease (LEAD). However, whether there is a difference in the performance among individual DCBs has not yet been fully discussed. The present sub-analysis of real-world data from a prospective trial of first-generation DCBs compared the clinical outcomes between high- and low-dose DCBs using propensity score matching methods. The primary endpoint was the restenosis-free and revascularization-free rates at 1 year.

Results
We compared 592 pairs matched for patient and lesion characteristics using propensity score matching among a total of 2,507 cases with first-generation DCBs (592 and 1,808 cases in the Lutonix low-dose and In.PACT Admiral high-dose DCB groups, respectively). There were no differences in patient/lesion characteristics, procedural success rates, or complications between the two groups. First-generation low-dose DCB had significantly lower patency (73.3% [95% confidence interval, 69.6%–77.3%] in the low-dose DCB group versus 86.2% [84.1%–88.3%] in the high-dose DCB group; P < 0.001) and revascularization-free (84.9% [81.9%–88.1%] versus 92.5% [90.8%–94.1%]; P < 0.001) rates. Chronic kidney disease on dialysis, cilostazol use, anticoagulant use, and severe calcification had a significant interaction effect in the association (all P < 0.05).

Conclusions
EVT to FPA with first-generation DCBs had inferior low-dose patency outcomes as compared with high-dose outcomes in the present cohort.

Level of evidence
Sub analysis of a prospective multicenter study.
Milestones in the history of neurocritical care
Authors: Kollmar Rainer , De Georgia Michael 
Published: August 2023

AbstractOver the last century, significant milestones have been achieved in managing critical illness and diagnosing and treating neurological diseases. Building upon these milestones, the field of neurocritical care emerged in the 1980 and 1990 s at the convergence of critical care medicine and acute neurological treatment. This comprehensive review presents a historical account of key developments in neurocritical care in both the United States and Europe, with a special emphasis on German contributions. The scope of the review encompasses: the foundations of neurocritical care, including post-operative units in the 1920s and 30s, respiratory support during the poliomyelitis epidemics in the 40 and 50 s, cardiac and hemodynamic care in the 60 and 70 s, and stroke units in the 80 and 90 s; key innovations including cerebral angiography, computed tomography, and intracranial pressure and multi-modal monitoring; and advances in stroke, traumatic brain injury, cardiac arrest, neuromuscular disorders, meningitis and encephalitis. These advances have revolutionized the management of neurological emergencies, emphasizing interdisciplinary teamwork, evidence-based protocols, and personalized approaches to care.
Costs of implementing a multi-site facilitation intervention to increase access to medication treatment for opioid use disorder
Authors: Garcia Carla C. , Bounthavong Mark , Gordon Adam J. , Gustavson Allison M. , Kenny Marie E. , Miller Wendy , Esmaeili Aryan , Ackland Princess E. , Clothier Barbara A. , Bangerter Ann , Noorbaloochi Siamak , Harris Alex H. S. , Hagedorn Hildi J. 
Published: August 2023

Abstract
Background
The United States has been grappling with the opioid epidemic, which has resulted in over 75,000 opioid-related deaths between April 2020 and 2021. Evidence-based pharmaceutical interventions (buprenorphine, methadone, and naltrexone) are available to reduce opioid-related overdoses and deaths. However, adoption of these medications for opioid use disorder has been stifled due to individual- and system-level barriers. External facilitation is an evidence-based implementation intervention that has been used to increase access to medication for opioid use disorder (MOUD), but the implementation costs of external facilitation have not been assessed. We sought to measure the facility-level direct costs of implementing an external facilitation intervention for MOUD to provide decision makers with estimates of the resources needed to implement this evidence-based program.

Methods
We performed a cost analysis of the pre-implementation and implementation phases, including an itemization of external facilitation team and local site labor costs. We used labor estimates from the Bureau of Labor and Statistics, and sensitivity analyses were performed using labor estimates from the Veterans Health Administration (VHA) Financial Management System general ledger data.

Results
The average total costs for implementing an external facilitation intervention for MOUD per site was $18,847 (SD 6717) and ranged between $11,320 and $31,592. This translates to approximately $48 per patient with OUD. Sites with more encounters and participants with higher salaries in attendance had higher costs. This was driven mostly by the labor involved in planning and implementation activities. The average total cost of the pre-implementation and implementation activities were $1031 and $17,816 per site, respectively. In the sensitivity analysis, costs for VHA were higher than BLS estimates likely due to higher wages.

Conclusions
Implementing external facilitation to increase MOUD prescribing may be affordable depending on the payer’s budget constraints. Our study reported that there were variations in the time invested at each phase of implementation and the number and type of participants involved with implementing an external facilitation intervention. Participant composition played an important role in total implementation costs, and decision makers will need to identify the most efficient and optimal number of stakeholders to involve in their implementation plans.
Fabrication and functional attributes of nanotherapeutically engineered system for co-administration of adapalene and lycopene for enhanced acne management: an in vitro and in vivo investigation
Authors: Jain Amit Kumar , Mehta Parul 
Published: August 2023

Abstract
Background
Acne vulgaris is caused due to several different biological, environmental and specific pathological factors, resulting in secretion of unregulated amount of sebum by the sebaceous glands. Acne is thought to be a minor, self-restraint, puberty leading and hormone-induced cosmetic disorder. It significantly affects the quality of life leading to social disengagement, anxiety, depression, necessitating its therapeutic management in due course of time. Thus, despite not being fatal it has social and mental impact on individual’s life. Pathogenesis of acne is complex and multifactorial involving diverse physiological, environmental factors and significant role of propionibacterium acne. Retinoids have been used as preferred first-line therapy for treatment of acne, but they have many limitations. Adapalene has found to be acting synergistically with other drugs like benzoyl peroxide, salicylic acid, clindamycin, etc., and various other topical antioxidants.

Results
The study investigates the formulation and characterization of novel nanostructured lipid vesicles (NLVs) co-loaded with adapalene and lycopene, through topical gel. The high-pressure homogenization method was used to prepare nanostructured lipid vesicles followed by incorporation into hydrophilic gel vehicle. This novel system was scientifically tested for various evaluation parameters like size and vesicle size distribution, polydispersity index, drug entrapment efficiency and in vitro drug release characteristics, along with rheological behavior of formulation gel. Skin permeation and biodistribution characteristics through skin were also evaluated, and better localization of drug in dermis and reduced systemic penetration were the key observation in prepared formulation.

Conclusion
The simultaneous co-administration of lycopene resulted in an additive effect in therapeutic management of acne. It can be predicted that this novel NLVs-based gel along with synergistic effect of lycopene will definitely result in better therapeutic efficacy and reduced systemic adverse effects of adapalene. This combination can be explored as future alternative for acne therapy.
Understanding post-pandemic travel intention toward rural destinations by expanding the theory of planned behavior (TPB)
Authors: Azhar Mohd , Nafees Sehar , Sujood  , Hamid Sheeba 
Published: August 2023

AbstractThis research intends to understand post-pandemic travel intention toward rural areas by extending the theory of planned behavior (TPB). Social media use (SMU) and electronic word of mouth (eWOM) have been incorporated into the original TPB model as additional constructs to increase its predictive power. To date, no study has measured post-pandemic travel intention in the Indian context utilizing a modified model of TPB taking the studied variables; thus, this study fills this void. A sample of 305 respondents was collected on a convenience basis via an online questionnaire. The targeted population of this study were the Indian social media users who follow the web pages of travel agencies. “SPSS 20” and “AMOS 22.0” were used for the statistical analysis. The results reveal that attitude (AT), subjective norm (SN), perceived behavioral control (PBC), social media usage (SMU) and electronic word of mouth (eWOM) all have a beneficial impact on post-pandemic travel intention toward rural destinations. These factors explained approximately 53% (R2 = 0.529) of the variance in the post-pandemic travel intention toward rural destinations. A number of theoretical and managerial ramifications can be deduced from the findings of this study. The novelty of this research lies in its integration of SMU and eWOM into the original TPB framework to assess individuals’ post-pandemic travel intentions toward rural destinations.