bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomic screening uncovers candidate diagnostic and monitoring serum biomarkers of ankylosing spondylitis
Authors: Hwang Mark , Assassi Shervin , Zheng Jim , Castillo Jessica , Chavez Reyna , Vanarsa Kamala , Mohan Chandra , Reveille John 
Published: April 2023

Abstract
Background
We sought to discover serum biomarkers of ankylosing spondylitis (AS) for diagnosis and monitoring disease activity.

Methods
We studied biologic-treatment-naïve AS and healthy control (HC) patients’ sera. Eighty samples matched by age, gender, and race (1:1:1 ratio) for AS patients with active disease, inactive disease, and HC were analyzed with SOMAscan™, an aptamer-based discovery platform. T-tests tests were performed for high/low-disease activity AS patients versus HCs (diagnosis) and high versus low disease activity (Monitoring) in a 2:1 and 1:1 ratio, respectively, to identify differentially expressed proteins (DEPs).
We used the Cytoscape Molecular Complex Detection (MCODE) plugin to find clusters in protein–protein interaction networks and Ingenuity Pathway Analysis (IPA) for upstream regulators. Lasso regression analysis was performed for diagnosis.

Results
Of the 1317 proteins detected in our diagnosis and monitoring analyses, 367 and 167 (317 and 59, FDR-corrected q < .05) DEPs, respectively, were detected. MCODE identified complement, IL-10 signaling, and immune/interleukin signaling as the top 3 diagnosis PPI clusters. Complement, extracellular matrix organization/proteoglycans, and MAPK/RAS signaling were the top 3 monitoring PPI clusters. IPA showed interleukin 23/17 (interleukin 22, interleukin 23A), TNF (TNF receptor-associated factor 3), cGAS-STING (cyclic GMP-AMP synthase, Stimulator of Interferon Gene 1), and Jak/Stat (Signal transducer and activator of transcription 1), signaling in predicted upstream regulators. Lasso regression identified a Diagnostic 13-protein model predictive of AS. This model had a sensitivity of 0.75, specificity of 0.90, a kappa of 0.59, and overall accuracy of 0.80 (95% CI: 0.61–0.92). The AS vs HC ROC curve was 0.79 (95% CI: 0.61–0.96).

Conclusion
We identified multiple candidate AS diagnostic and disease activity monitoring serum biomarkers using a comprehensive proteomic screen. Enrichment analysis identified key pathways in AS diagnosis and monitoring. Lasso regression identified a multi-protein panel with modest predictive ability.
Autologous mesenchymal stem cell transplantation for spinal fusion: 10 years follow-up of a phase I/II clinical trial
Authors: Gomez-Ruiz Victoria , Blanco Juan F. , Villarón Eva M. , Fidalgo Helena , López-Parra Miriam , Sánchez-Guijo Fermín 
Published: April 2023

AbstractPosterolateral spinal fusion is the standard surgical approach for patients with degenerative disc disease. In our previously published article, we reported a 5-years follow-up of a phase I/II clinical trial in patients undergoing spinal fusion with autologous mesenchymal stem cells (MSCs) embedded in tricalcium phosphate. In the current manuscript, we have updated the results with a 10-year follow-up, the longest reported to date in this setting. After clinical and radiological evaluation, safety of the procedure was further confirmed in all 11 treated patients, with no evidence of tumor, infection, inflammatory reaction, or heterotopic ossification related to the administration of MSCs. Regarding clinical efficacy, low back pain and radicular pain (both assessed by the visual analogue scale—VAS), and the Owestry Disability Index remained significantly lower compared to pre-intervention. Radiologic evaluation demonstrated spinal fusion in all cases, improving over time. Finally, quality of life improved significantly also during follow-up. In summary, the use of tricalcium phosphate-embedded autologous MSCs with lumbar posterolateral arthrodesis is safe and potentially provides long-term benefits for 10 years.
Improving the response to oxaliplatin by targeting chemotherapy-induced CLDN1 in resistant metastatic colorectal cancer cells
Authors: Cherradi Sara , Garambois Véronique , Marines Johanna , Andrade Augusto Faria , Fauvre Alexandra , Morand Olivia , Fargal Manon , Mancouri Ferial , Ayrolles-Torro Adeline , Vezzo-Vié Nadia , Jarlier Marta , Loussaint Gerald , Huvelle Steve , Joubert Nicolas , Mazard Thibault , Gongora Céline , Pourquier Philippe , Boissière-Michot Florence , Rio Maguy Del 
Published: April 2023

Abstract
Background
Tumor resistance is a frequent cause of therapy failure and remains a major challenge for the long-term management of colorectal cancer (CRC). The aim of this study was to determine the implication of the tight junctional protein claudin 1 (CLDN1) in the acquired resistance to chemotherapy.

Methods
Immunohistochemistry was used to determine CLDN1 expression in post-chemotherapy liver metastases from 58 CRC patients. The effects of oxaliplatin on membrane CLDN1 expression were evaluated by flow cytometry, immunofluorescence and western blotting experiments in vitro and in vivo. Phosphoproteome analyses, proximity ligation and luciferase reporter assays were used to unravel the mechanism of CLDN1 induction. RNAseq experiments were performed on oxaliplatin-resistant cell lines to investigate the role of CLDN1 in chemoresistance. The “one-two punch” sequential combination of oxaliplatin followed by an anti-CLDN1 antibody-drug conjugate (ADC) was tested in both CRC cell lines and murine models.

Results
We found a significant correlation between CLDN1 expression level and histologic response to chemotherapy, CLDN1 expression being the highest in resistant metastatic residual cells of patients showing minor responses. Moreover, in both murine xenograft model and CRC cell lines, CLDN1 expression was upregulated after exposure to conventional chemotherapies used in CRC treatment. CLDN1 overexpression was, at least in part, functionally related to the activation of the MAPKp38/GSK3β/Wnt/β-catenin pathway. Overexpression of CLDN1 was also observed in oxaliplatin-resistant CRC cell lines and was associated with resistance to apoptosis, suggesting an anti-apoptotic role for CLDN1. Finally, we demonstrated that the sequential treatment with oxaliplatin followed by an anti-CLDN1 ADC displayed a synergistic effect in vitro and in in vivo.

Conclusion
Our study identifies CLDN1 as a new biomarker of acquired resistance to chemotherapy in CRC patients and suggests that a “one-two punch” approach targeting chemotherapy-induced CLDN1 expression may represent a therapeutic opportunity to circumvent resistance and to improve the outcome of patients with advanced CRC.
State of the art in research on the gut-liver and gut-brain axis in poultry
Authors: Beldowska Aleksandra , Barszcz Marcin , Dunislawska Aleksandra 
Published: April 2023

AbstractThe relationship between the intestines and their microbiota, the liver, and the neuronal system is called the gut-liver-brain axis. This relationship has been studied and observed for a relatively short time but is considered in the development of research focused on, e.g., liver diseases and intestinal dysbiosis. The role of the gut microbiota in this relationship is crucial, as it acts on poultry’s performance and feed utilization, affecting meat and egg quality. The correct composition of the intestinal microbiota makes it possible to determine the essential metabolic pathways and biological processes of the individual components of the microbiota, allowing further speculation of the role of microbial populations on internal organs such as the liver and brain in the organism. The gut microbiota forms a complex, dense axis with the autonomic and enteric nervous systems. The symbiotic relationship between the liver and gut microbiota is based on immune, metabolic and neuroendocrine regulation, and stabilization. On the other hand, the gut-brain axis is a bidirectional interaction and information transfer system between the gastrointestinal tract and the central nervous system. The following paper will discuss the current state of knowledge of the gut-liver-brain axis of poultry, including factors that may affect this complex relationship.
The psychometric properties of positive and negative beliefs about the rumination scale in Chinese undergraduates
Authors: Zhou Honggui , Liu Hong , Ma Xiaohong , Deng Yunlong 
Published: April 2023

AbstractBackgroundRumination, a transdiagnostic factor in different psychopathological conditions, is believed to be activated and sustained by dysfunctional metacognition. The Positive Beliefs about Rumination Scale (PBRS) and the Negative Beliefs about Rumination Scale (NBRS) have been used to measure the metacognitive beliefs of rumination and have been investigated in many cultural contexts. However, it remains unclear whether these scales can work as well for the Chinese population. Therefore, this study aimed to explore the psychometric properties of the Chinese versions of these scales and to test the metacognitive model of rumination for students with different levels of depression.MethodsThe PBRS and NBRS were forward-backward translated into Mandarin. In total 1,025 college students were recruited to complete a battery of web-based questionnaires. Exploratory factor analysis, confirmatory factor analysis, and correlation analysis were used to test the structure, validity, and reliability of the two scales, as well as their item correlations with rumination.ResultsA new two-factor structure of the PBRS (rather than the original one-factor model) and a new three-factor structure of the NBRS (rather than the original two-factor model) were extracted. The goodness-of-fit indices of these two factor models showed they had a good to very good fit with the data. The internal consistency and construct validity of PBRS and NBRS were also affirmed.ConclusionThe Chinese versions of the PBRS and the NBRS were generally shown to be reliable and valid, but their newly extracted structures fit the Chinese college students better than their original structures. These new models of PBRS and NBRS are of value to be further explored in Chinese population.
Surgical management of a giant paratubal cyst: a case report and review of the literature
Authors: Archer S. , Alaniz V. I. , Huguelet P. S. 
Published: April 2023

Abstract
Background
Surgical management of giant paratubal cysts can be challenging in the pediatric and adolescent population. Larger adnexal masses can heighten suspicion for malignancy, placing the ovary at risk of unwarranted removal, and larger masses sometimes necessitate larger surgical incisions which can increase postoperative pain and delay return to school and regular activities. We present the evaluation and management of a giant paratubal cyst containing 16 l of fluid in an adolescent, using a mini-laparotomy approach.

Case presentation
A 16-year-old patient presented with 1-month history of worsening nausea and increasing abdominal girth. Abdominal imaging revealed a 42-cm adnexal mass. A modified drainage technique with a mini-laparotomy incision was utilized, thereby preventing tumor spill while minimizing size of the surgical incision. Postoperative recovery was complicated by prolonged nausea, but the patient was discharged home 2 days after surgery and returned to school the following week.

Conclusion
This case illustrates that giant adnexal masses can be managed using a mini-laparotomy approach, thereby expediting return to regular activities.
Understanding the relationship between oral health and psychosis: qualitative analysis
Authors: Turner Elizabeth , Berry Katherine , Quinlivan Leah , Shiers David , Aggarwal Vishal , Palmier-Claus Jasper 
Published: April 2023

Background
Individuals with psychosis have poor oral health compared with the general population. The interaction between oral health and psychosis is likely to be complex and have important ramifications for improving dental and mental health outcomes. However, this relationship is poorly understood and rarely studied using qualitative methods.

Aims
To explore patient perspectives on the relationship between oral health and psychosis.

Method
The authors recruited 19 people with experiences of psychosis from community mental health teams, early intervention in psychosis services, and rehabilitation units. Participants completed a qualitative interview. Transcripts were analysed with reflexive thematic analysis.

Results
The analysis resulted in three themes: theme 1, psychosis creates barriers to good oral health, including a detachment from reality, the threat of unusual experiences and increased use of substances; theme 2, the effects of poor oral health in psychosis, with ramifications for self-identify and social relationships; and theme 3, systems for psychosis influence oral health, with central roles for formal and informal support networks.

Conclusions
Psychosis was perceived to affect adherence to oral health self-care behaviours and overall oral health. Poor oral health negatively affected self-identity and social relationships. Clinical implications include a systemic approach to provide early intervention and prevention of the sequelae of dental disease, which lead to tooth loss and impaired oral function and aesthetics, which in turn affect mental health. Participants felt that mental health services play an important role in supporting people with oral health.
Tuberculosis of Adrenal Glands—A Population-based Case-control Study
Authors: Lindh Jonatan D , Patrova Jekaterina , Rushworth R Louise , Mannheimer Buster , Falhammar Henrik 
Published: April 2023

Abstract

Purpose
Adrenal tuberculosis (ATB) can cause primary adrenal insufficiency (PAI) or may be misdiagnosed as nonfunctional adrenal tumors (NFATs) in patients with tuberculosis. Very little is known about its epidemiology in a modern, high-income setting. The aim was to investigate adrenal involvement and associated mortality in patients with tuberculosis.

Methods
By using national registers, patients with tuberculosis and adrenal lesions were compared with controls without adrenal tumors. To analyze mortality in individuals with ATB or possible adrenal affection (ie, tuberculosis and NFAT), a subgroup of controls with tuberculosis was selected. The study population was included from 2005 to 2019 and followed until death or 2020. In mortality adjustments were made for age and sex.

Results
Eight patients with ATB, 23 232 patients with NFAT, and 144 124 controls were included. Among those with NFAT, we found 34 with tuberculosis and NFAT. Among controls, 129 individuals diagnosed with tuberculosis were identified. The risk of having an adrenal tumor was increased in tuberculosis (odds ratio, 1.64; 95% CI, 1.12-2.39). Of those with ATB, 7 (88%) had PAI. One patient (3%) with tuberculosis and NFAT and 1 (0.8%) control with tuberculosis had PAI. Compared with controls with tuberculosis, mortality was increased in patients with ATB (hazard ratio, 5.4; 95% CI, 2.2-13.2; adjusted hazard ratio, 6.2; 95% CI, 2.5-15.6), and in patients with tuberculosis and NFAT (1.3; 0.6-2.7; 2.3; 1.1-5.1). PAI was a contributing factor in 4/6 (67%) deaths in patients with ATB.

Conclusions
Tuberculosis with adrenal lesions was extremely rare. Most patients with ATB had PAI and mortality was increased.
Diagnostic Evaluation of Stroke Etiology
Authors: Meschia James F. 
Published: April 2023

ABSTRACT

OBJECTIVE
Precise therapies require precise diagnoses. This article provides an evidence-based approach to confirming the diagnosis of ischemic stroke, characterizing comorbidities that provide insights into the pathophysiologic mechanisms of stroke, and identifying targets for treatment to optimize the prevention of recurrent stroke.

LATEST DEVELOPMENTS
Identifying the presence of patent foramen ovale, intermittent atrial fibrillation, and unstable plaque is now routinely included in an increasingly nuanced workup in patients with stroke, even as ongoing trials seek to clarify the best approaches for treating these and other comorbidities. Multicenter trials have demonstrated the therapeutic utility of patent foramen ovale closure in select patients younger than age 60 years. Insertable cardiac monitors detect atrial fibrillation lasting more than 30 seconds in about one in ten patients monitored for 12 months following a stroke. MRI of carotid plaque can detect unstable plaque at risk of being a source of cerebral embolism.

ESSENTIAL POINTS
To optimize the prevention of recurrent stroke, it is important to consider pathologies of intracranial and extracranial blood vessels and of cardiac structure and rhythm as well as other inherited or systemic causes of stroke. Some aspects of the stroke workup should be done routinely, while other components will depend on the clinical circumstances and preliminary testing results.
The Caenorhabditis elegans anchor cell transcriptome: ribosome biogenesis drives cell invasion through basement membrane
Authors: Costa Daniel S. , Kenny-Ganzert Isabel W. , Chi Qiuyi , Park Kieop , Kelley Laura C. , Garde Aastha , Matus David Q. , Park Junhyun , Yogev Shaul , Goldstein Bob , Gibney Theresa V. , Pani Ariel M. , Sherwood David R. 
Published: April 2023

ABSTRACT
Cell invasion through basement membrane (BM) barriers is important in development, immune function and cancer progression. As invasion through BM is often stochastic, capturing gene expression profiles of actively invading cells in vivo remains elusive. Using the stereotyped timing of Caenorhabditis elegans anchor cell (AC) invasion, we generated an AC transcriptome during BM breaching. Through a focused RNAi screen of transcriptionally enriched genes, we identified new invasion regulators, including translationally controlled tumor protein (TCTP). We also discovered gene enrichment of ribosomal proteins. AC-specific RNAi, endogenous ribosome labeling and ribosome biogenesis analysis revealed that a burst of ribosome production occurs shortly after AC specification, which drives the translation of proteins mediating BM removal. Ribosomes also enrich near the AC endoplasmic reticulum (ER) Sec61 translocon and the endomembrane system expands before invasion. We show that AC invasion is sensitive to ER stress, indicating a heightened requirement for translation of ER-trafficked proteins. These studies reveal key roles for ribosome biogenesis and endomembrane expansion in cell invasion through BM and establish the AC transcriptome as a resource to identify mechanisms underlying BM transmigration.
Pten inhibition dedifferentiates long-distance axon-regenerating intrinsically photosensitive retinal ganglion cells and upregulates mitochondria-associated Dynlt1a and Lars2
Authors: Rheaume Bruce A. , Xing Jian , Lukomska Agnieszka , Theune William C. , Damania Ashiti , Sjogren Greg , Trakhtenberg Ephraim F. 
Published: April 2023

ABSTRACT
Central nervous system projection neurons fail to spontaneously regenerate injured axons. Targeting developmentally regulated genes in order to reactivate embryonic intrinsic axon growth capacity or targeting pro-growth tumor suppressor genes such as Pten promotes long-distance axon regeneration in only a small subset of injured retinal ganglion cells (RGCs), despite many RGCs regenerating short-distance axons. A recent study identified αRGCs as the primary type that regenerates short-distance axons in response to Pten inhibition, but the rare types which regenerate long-distance axons, and cellular features that enable such response, remained unknown. Here, we used a new method for capturing specifically the rare long-distance axon-regenerating RGCs, and also compared their transcriptomes with embryonic RGCs, in order to answer these questions. We found the existence of adult non-α intrinsically photosensitive M1 RGC subtypes that retained features of embryonic cell state, and showed that these subtypes partially dedifferentiated towards an embryonic state and regenerated long-distance axons in response to Pten inhibition. We also identified Pten inhibition-upregulated mitochondria-associated genes, Dynlt1a and Lars2, which promote axon regeneration on their own, and thus present novel therapeutic targets.
Technische Vorbemerkungen zur Erforschung einer spätkeltischen Wallanlage
Authors: Richner Kaspar , Stegmüller Christian 
Published: April 2023

Der Erhaltungszustand des Basler Murus Gallicus verlangte nach einer besonderen Sondiermethode: Während die Steine der Trockenmauer an der Wallfront nicht übersehen werden können, sind die Balken der Holzarmierung des Wallinnern im trockenen Boden nur noch als Negative (Hohlräume oder lockere Zonen in der Wallschüttung) erhalten.
Es ist deshalb von grösster Wichtigkeit, die Balkennegative bereits vor dem Freilegen in der Fläche zu erkennen. Hierzu wird die Abbaufläche mit einer Sonde systematisch abgesucht.
Providing information about a child's oncological disease - a challenge for a pediatric oncologist
Authors: Pawłowski Piotr , Ziętara Karolina , Michalczyk Justyna , Fryze Magdalena , Kościołek Aneta 
Published: April 2023

Introduction: The diagnosis of a serious illness in a family affects all members. The child is a special subject, as he cannot yet understand many aspects of life at such a high perceptual level as an adult. Communicating difficult information is part of the doctor's task and is one of the most difficult parts of the therapeutic process.
The aim of the study: To analyze the methodology of communicating difficult information to pediatric oncology patients.
Material and method: Polish and English-language scientific literature from 2012 - 2022 from databases such as PubMed, SCOPUS, Google Scholar, Web of Science was reviewed. Searches were conducted according to keywords: communicating difficult information, oncology, pediatrics, physician.
Results and conclusions: One of the most well-known protocols for communicating difficult information is SPIKES. It describes the basic and necessary steps for properly informing parents and the child about the diagnosis, for example. Healthy siblings should also learn about their brother's illness, but this task falls to the parents. Psycho-oncological support for both the child and parents should be holistic, based on best practices, and tailored to the family's needs.
Obesity as a multidimensional problem in the health care system - literature review
Authors: Grzybowski Jakub , Walczak Patrycja , Hordejuk Michalina , Marczak Aleksandra , Hyjek Michał , Szymkiewicz Karolina , Walczak Klaudia 
Published: April 2023

Awareness among medical professionals and patients about the impact of obesity on health is currently at a good level. Medical recommendations often include the recommendation of weight reduction, and there are increasing numbers of dieticians on wards educating patients about a healthy diet and physical activity. Special programs are also being developed to provide pharmacological and surgical treatment for obesity. New drugs that reduce body fat are becoming available. Unfortunately, as statistics show, the problem is still global and the number of obese people is increasing over the years. Currently, there is a huge emphasis on treating and reversing the effects of obesity, rather than preventing it. Scientific and technological advances are lagging behind in the face of the challenges posed by the large obese population.
In this work, we present the radiological, anesthesiological and surgical problems and limitations associated with obesity, which may not be obvious to those who do not deal with obese patients on a daily basis as well as the patients themselves.