bluuebunny/crossref_metadata_2025_split huggingface dataset:
The 4C’s of influence framework: fostering leadership development through character, competence, connection and culture
Authors: Do Victor , Maniate Jerry M. , Sultan Nabil , Sonnenberg Lyn 
Published: January 2023

Purpose
The purpose of this paper is to describe the 4C's of Infuence framework and it's application to medicine and medical education. Leadership development is increasingly recognised as an integral physician skill. Competence, character, connection and culture are critical for effective influence and leadership. The theoretical framework, “The 4C’s of Influence”, integrates these four key dimensions of leadership and prioritises their longitudinal development, across the medical education learning continuum.

Design/methodology/approach
Using a clinical case-based illustrative model approach, the authors provide a practical, theoretical framework to prepare physicians and medical learners to be engaging influencers and leaders in the health-care system.

Findings
As leadership requires foundational skills and knowledge, a leader must be competent to best exert positive influence. Character-based leadership stresses development of, and commitment to, values and principles, in the face of everyday situational pressures. If competence confers the ability to do the right thing, character is the will to do it consistently. Leaders must value and build relationships, fostering connection. Building coalitions with diverse networks ensures different perspectives are integrated and valued. Connected leadership describes leaders who are inspirational, authentic, devolve decision-making, are explorers and foster high levels of engagement. To create a thriving, learning environment, culture must bring everything together, or will become the greatest barrier.

Originality/value
The framework is novel in applying concepts developed outside of medicine to the medical education context. The approach can be applied across the medical education continuum, building on existing frameworks which focus primarily on what competencies need to be taught. The 4C’s is a comprehensive framework for practically teaching the leadership for health care today.
Design and synthesis of thermosensitive target segment resin with precisely controlled distance between immobilization sites for immobilization of penicillin G acylase
Authors: Liu Yangdong , Lu Siyuan , Tu Hongyi , Zhang Boyuan , Zhao Yaqin , He Jiasheng , He Liangliang , Chen Zhenbin 
Published: January 2023

Purpose
To save the economic cost and improve the performance of enterprises, this study aims to synthesize high performance immobilized penicillin G acylase (PGA) carriers with fast reaction speed, high recovery rate of enzyme activity and good reusability through corresponding theoretical guidance and experimental exploration.

Design methodology approach
A diblock resin was synthesized by reversible addition-fragmentation chain transfer polymerization method using N, N-diethylacrylamide (DEA) and β-hydroxyethyl methacrylate (HEMA) as functional monomers poly(N, N-diethylacrylamide)-b-poly(β-hydroxyethyl methacrylate) (PDEA-b-PHEMA) was obtained, and the effect of the ratio of DEA and HEMA on the activity of PGA was investigated, and the appropriate block ratio of DEA and HEMA was obtained. After that, the competitive rate of HEMA and glycidyl methacrylate (GMA) under the carrier preparation conditions was investigated. Based on the above work, a thermosensitive resin carrier PDEA-b-PHEMA-b-P(HEMA-co-GMA) with different target distances was synthesized, and the chemical structures and molecular weight of copolymers were investigated by hydrogen NMR (1H NMR).

Findings
The lower critical solution temperature of the resin support decreases with the increase of the monomer HEMA in the random copolymerization; the catalytic performance study indicated that the response rate of the immobilized PGA is fast, and the recovery rate of the enzyme activity of the immobilized PGA varies with the distance between the targets. When the molar ratio of HEMA to GMA in the resin block is 8.15:1 [i.e. resin PDEA100-b-PHEMA10-b-P(HEMA65-co-GMA8)], the activity recovery rate of immobilized PGA can reach 50.51%, which was 15.49% higher than that of pure GMA immobilized PGA.

Originality value
This contribution provides a novel carrier for immobilizing PGA. Under the optimal molar ratio, the enzyme activity recovery could be up to 50.51%, which was 15.49% higher than that of PGA immobilized on the carrier with nonregulated distance between two immobilization sites.
Antecedents and outcomes of brand pride: moderating role of narcissism
Authors: Nandy Subarna , Sondhi Neena , Joshi Himanshu 
Published: January 2023

PurposeThis paper aims to draw on the appraisal theory and the theory of self-brand congruence (SBC) to study the multidimensional emotion of brand pride. It conceptualizes and validates the relationship of brand pride with SBC, brand loyalty and oppositional brand loyalty and establishes the role of narcissism as a moderator.Design/methodology/approachStandardized scales, including a new brand pride scale developed by the authors, were used to collect data from 522 respondents. Covariance-based structural equation modeling was used to test the conceptual model. Multi-group moderation analysis tested the differences in the proposed relationship between high and low narcissists.FindingsResults posit brand pride as a multidimensional construct with SBC as its significant antecedent. The findings also support most hypothesized relationships between brand pride and behavioral outcomes. In addition, the study confirms the moderating effect of narcissism on the relationship between brand pride dimensions and brand loyalty and opposition brand loyalty.Research limitations/implicationsThe study sample was from a developing nation – India. Similar cohorts from developing and developed countries could provide a unique cross-nation comparison.Practical implicationsThe role of brand pride in impacting brand loyalty and oppositional brand loyalty has significant implications for practice. Marketing communication to inculcate brand pride among consumers will significantly impact the brand’s profitability.Originality/valueValidation of SBC as a precursor to brand pride and the relationship of brand pride with brand loyalty and oppositional brand loyalty contributes significantly to branding theory and practice. This study also establishes narcissism as a moderator between brand pride and loyalty outcomes.
Reconstructing spatiotemporal dynamics of mixed conifer and broad‐leaved forests with a spatially explicit individual‐based dynamic vegetation model
Authors: Sato Hisashi , Shibuya Masato , Hiura Tsutom 
Published: January 2023

AbstractA dynamic vegetation model was adapted to a mixed forest of deciduous broad‐leaved trees and evergreen conifers in Hokkaido, the northernmost main island of Japan, located within a transition zone between cool‐temperate and sub‐boreal forest ecosystems. In this forest, elevation and a terrestrial‐wetness index affect tree biomass and the percentage of conifers. We maximized agreement between this observed pattern and model output by calibrating parameters that control drought and excessive soil‐moisture tolerance, establishment rate, and background mortality rate. In the simulation, biomass increased as a single peak curve with simulation year, while the percentage of conifers increased until the end of 200 simulation years. The 75‐year simulated forest was most comparable to the adapted forest, consistent with the average frequency of catastrophic storm disturbances in Hokkaido. The model also reconstructed a reasonable succession pattern based on temperature and soil moisture. Therefore, the model mechanistically reconstructed the mixed forest via spatial niche segregation and succession after catastrophic disturbances. However, the model did not reconstruct the percentages of broad‐leaved trees and conifers of forests prior to the disturbance in independent validation plots, demonstrating that additional processes should be considered in future models.
Affordable de novo generation of fish mitogenomes using amplification‐free enrichment of mitochondrial DNA and deep sequencing of long fragments
Authors: Ramón‐Laca Ana , Gallego Ramón , Nichols Krista M. 
Published: January 2023

AbstractBiomonitoring surveys make use of metabarcoding tools to describe the community composition. These studies match their sequencing results against public genomic databases to identify the species. However, mitochondrial genomic reference data are yet incomplete, only a few genes may be available, or the suitability of existing sequence data is suboptimal for species level resolution. Here, we present a dedicated and cost‐effective workflow with no DNA amplification for generating complete fish mitogenomes for the purpose of strengthening fish mitochondrial databases. Two different strategies using long fragment sequencing with Oxford Nanopore technology coupled with mitochondrial DNA enrichment were used. One where the enrichment is achieved by preferential isolation of mitochondria followed by DNA extraction and nuclear DNA depletion (“mitoenrichment”). A second enrichment approach takes advantage of the CRISPR Cas9 targeted scission on previously dephosphorylated DNA (“targeted mitosequencing”). The sequencing results varied between tissue, species, and integrity of the DNA. The mitoenrichment method yielded 0.17%–12.33% of sequences on target and a mean coverage ranging from 74.9 to 805‐fold. The targeted mitosequencing experiment from native genomic DNA yielded 1.83%–55% of sequences on target and a 38 to 2123‐fold mean coverage. These produced complete mitogenomes of species with homopolymeric regions, tandem repeats, and gene rearrangements. We demonstrate that deep sequencing of long fragments of native fish DNA can be achieved with low computational resources in a cost‐effective manner, opening the discovery of mitogenomes of nonmodel or understudied fish taxa to a broad range of laboratories worldwide.
Less aggressive treatment for less aggressive disease? A retrospective single‐center study of pulmonary‐limited metastases associated with colorectal cancer
Authors: Qu Wang , Sun Yongkun , Zhang Wen , Jiang Zhichao , Han Yue , Jin Jing , Xue Qi , Zhou Aiping 
Published: January 2023

AbstractObjectiveTo explore the appropriate treatment strategies, clinical outcomes, and prognostic factors of patients with pulmonary‐limited metastases derived from colorectal cancer (CRC), usually manifested as a less aggressive course.MethodsA retrospective review was conducted in 331 CRC patients diagnosed with pulmonary‐limited metastases at a single institution between January 2011 and November 2017. The Kaplan–Meier method was used to calculate the overall survival (OS). Further analysis was conducted according to treatment modalities. Univariate and multivariate analyses were used to determine potential prognostic factors influencing OS.ResultsWith a median follow‐up time of 38.6 months, the median OS in all patients was 45.2 months. A total of 163 patients received intensive local treatment with a median OS of 76.4 months, whereas 168 patients received palliative systemic treatment with a median OS of 29.7 months. The median OS was 68.9 months for patients treated with radiotherapy/radiofrequency ablation, with similar efficacy compared to surgery group, whose OS had not reached yet. No survival benefits were observed from the additional targeted therapy in systemic treatment group. The prognostic analysis demonstrated unilateral/bilateral lesions, synchronous/metachronous metastases, intensive local treatment, and resection of primary lesion that were significantly associated with survival of patients.ConclusionsIntensive local treatment alone for pulmonary lesions was associated with excellent survival in certain patients with CRC presented with metastases confined to lungs. Doublet systemic chemotherapy as the first‐line treatment also revealed satisfied efficacy and safety.
Comparison of cyclosporine and systemic corticosteroid for treating drug reaction with eosinophilia and systemic symptoms syndrome: A retrospective 20‐year single‐centre study in South Korea
Authors: Kwon Hyeok‐Jin , Yoon Jung‐Ho 
Published: January 2023

AbstractBackground/ObjectivesDrug reaction with eosinophilia and systemic symptoms (DRESS) syndrome is a potentially life‐threatening hypersensitive disorder. Cyclosporine has been indicated for adverse cutaneous drug eruptions. However, studies evaluating its clinical effectiveness in DRESS syndrome have been rare. This study aimed to evaluate the clinical efficacy of cyclosporine in DRESS syndrome compared to that of systemic corticosteroids.MethodsIn the cyclosporine group, oral cyclosporine was administered twice a day for a total of 2–3 mg/kg/day for 1 week, and subsequently reduced to 1–1.5 mg/kg/day for extended treatment. In the corticosteroid group, intravenous or oral methylprednisolone was administered at 1–1.5 mg/kg/day for 1 week, with variable tapering plans. Laboratory changes before and after treatment, hospitalized days, treatment periods, and time to normalization from clinical manifestations in each group were statistically evaluated. Adverse effects of these regimens were observed during the entire treatment period.ResultsEighty patients were enrolled in this retrospective study. The cyclosporine and corticosteroid group had 27 and 53 patients, respectively. Total leucocyte and eosinophil counts, liver enzymes, and C‐reactive proteins were significantly decreased after treatment in both groups. There were no statistically significant differences observed in hospitalized days, treatment period, and time to normalization from clinical manifestations between the two groups. The corticosteroid group experienced relatively more adverse effects than the cyclosporine group.ConclusionsCyclosporine was discovered to be clinically effective in DRESS syndrome and this study suggests that cyclosporine could be a feasible primary therapeutic option for DRESS syndrome.
MicroRNAs expression in semen and testis of azoospermic men
Authors: Wainstein Amit , Hassan Sarah , Barda Shimi , Lehavi Ofer , Azem Foad , Ben‐Dov Iddo Z. , Hauser Ron , Kleiman Sandra E. 
Published: January 2023

AbstractBackgroundMicroRNAs are involved in the regulation of spermatogenesis, are detected in semen and may be useful as molecular markers for predicting residual complete spermatogenesis in azoospermic men.ObjectivesTo study the biomarker potential of microRNAs that are detected in semen and testicular tissue.Materials and methodsMicroRNA profiles were analyzed in semen fractions of normozoospermic (n = 3) and azoospermic (n = 6) men by small RNA deep sequencing. Specific microRNAs were further analyzed by reverse transcription and quantitative polymerase chain reaction in eight testicular samples and 46 semen supernatants. The semen supernatant samples included 18 normozoospermic and 28 azoospermic men with various pathologies.ResultsThe sequenced microRNA profiles of semen supernatant fraction samples were distinct from the other fractions. Significant expression differences were observed between the semen supernatant of normozoospermic and azoospermic men. Further analysis by reverse transcription and quantitative polymerase chain reaction revealed that expression of miR‐202‐3p was considerably reduced (undetectable in most samples) in the azoospermic semen supernatants. The expression of miR‐202‐3p was significantly lower in the azoospermic specimens than in the normozoospermic specimens and a trend was observed for miR‐629‐5p (p = 0.03 and 0.06, respectively). Differences in expression levels in the semen supernatant were observed among the various pathologies but not to a level of significance, possibly because of the small subgroups. miRNA‐370‐3p was significantly higher in semen supernatant samples from azoospermic men without sperm cells in testis (p = 0.05). In testes, the three microRNAs were expressed at higher levels in the obstructive and spermatocyte maturation arrest pathologies than in mixed atrophy and Sertoli cell only. miR‐202‐3p was detected in all testicular samples.ConclusionsMicroRNA expression profiles in semen were distinguishable between azoospermic and normozoospermic men. The microRNA profile also diverged among azoospermic men subdivided according to their testicular pathologies. The levels of specific microRNAs in testis and in the semen supernatant were not directly correlated.
Post cholecystectomy benign biliary stricture‐isolated hepatic duct stricture: a proposed modification of the BISMUTH classification
Authors: Rymbai Manbha L. , Paul Anoop , M. J. Aparna , Anantrao Atram Soham , John Reetu , Simon Betty , Joseph Anjilivelil J. , Raju Ravish Sanghi , Sitaram Venkatramani , Joseph Philip 
Published: January 2023

AbstractBackgroundPost‐cholecystectomy, benign biliary strictures are challenging for both patients and surgeons. Bismuth classified benign biliary strictures into 5 types. This study aimed to review these isolated hepatic duct strictures which were not included in Bismuth classification.MethodsThe case records of all patients who presented with post‐cholecystectomy benign biliary strictures between January 2005 and December 2020 at our centre were reviewed. Data regarding demography, type of stricture, and treatment strategy were entered into the standard proforma.ResultsThere were 242 patients [type I—3.7%, type II—41.7%, type III—38.0%, type IV—6.6%, and type V—7.8%]. Five (2.1%) patients did not fit the Bismuth classification and were the focus of this study. In each of these patients, an isolated hepatic duct stricture (first‐or second‐order hepatic duct) was present, with no involvement of the common hepatic duct or hilar confluence.ConclusionsThe addition of isolated hepatic duct stricture [type VI] to the Bismuth classification will enhance the original classification, help in reporting and management of this sub‐set of patients.
Formation of academic self‐concept and intrinsic value within and across three domains: Extending the reciprocal internal/external frame of reference model
Authors: Arens A. Katrin , Niepel Christoph 
Published: January 2023

AbstractBackgroundThe reciprocal internal/external frame of reference (RI/E) combines two models of academic self‐concept formation, namely the reciprocal effects model (REM) and the internal/external frame of reference (I/E) model. The REM assumes reciprocal relations between achievement and academic self‐concept. The I/E model assumes contrast effects between achievement and self‐concept across math and verbal domains. The RI/E model can be extended to more school subjects than one math and one verbal domain, and to other motivational constructs.AimsWe examined an extended RI/E model considering achievement, academic self‐concept, and intrinsic value related to math, German and English.SampleThe sample consisted of 1939 German secondary school students.MethodsThe three measurement waves covered Grades 5 to 7. Cross‐lagged panel models were used to analyse the data.ResultsWithin domains, there were reciprocal relations between achievement and academic self‐concept but only unidirectional relations between former achievements and later intrinsic values. Across domains, there were significant negative relations between former math achievement and later self‐concepts and intrinsic values in German and English, and between former German and English achievements and later self‐concept and intrinsic value in math. These findings imply contrast effects across math and verbal domains. The pattern of relations among constructs was found to be generalizable across gender and school tracks and stable across measurement waves.ConclusionThe study provides further support for the validity of the RI/E model and its extension to English as another school subject and intrinsic value as another motivational construct.
Clinical and molecular characteristics of forty Chinese children with essential thrombocythemia: A single‐center, retrospective analysis
Authors: Zhang Luyang , Chen Xiaoli , Hu Tianyuan , Xu Zefeng , Yang Wenyu , Fu Rongfeng , Zhang Lei , Zhu Xiaofan 
Published: January 2023

SummaryDue to the infrequency of essential thrombocythemia (ET) in children, little is known about its pathophysiological mechanism. To learn about the clinical and molecular features of Chinese children with ET, we retrospectively analysed 40 children with ET in a single center from 2015–2021. More than half of the children (51.3%, 20/39) were asymptomatic at diagnosis. Nearly half of the children (48.7%, 19/39) had microvascular symptoms, including headache, dizziness, stomachache, and paresthesia. Only two cases experienced vascular events. The proportion of children with typical “driver gene mutations” (i.e., JAK2 p.V617F, CALR exon 9, or MPL exon 10 mutation) was low (12.5%, 5/40). The equivalent ratio of children carried atypical driver gene mutations; however, 30 (75%) patients did not harbour driver gene mutations. Children carrying JAK2 p.V617F had lower platelet count (938 × 109/L vs. 1654 × 109/L, p = 0.031) compared to those without driver gene mutations. Cases harbouring typical driver mutations had higher median WBC counts than those without driver gene mutations (15.14 × 109/L vs. 8.01 × 109/L, p = 0.015). Compared to those without driver gene mutations, cases carrying typical and atypical driver gene mutations were both younger (median ages were 12, 6, and 7 years old, respectively; p = 0.023). The most prevalent non‐driver gene mutations and those mutations with prognostic significance in adult counterparts were less common in children with ET compared to adults with ET.
PERK‐STING‐RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis‐associated encephalopathy
Authors: Xiaofeng Guo , You Wu , Qi Jia , Hongwei Ma , Zhongmin Fan , Shiquan Wang , Lixia Du , Yuliang Peng , Zongping Fang , Xijing Zhang 
Published: January 2023

AbstractAimsSepsis‐associated encephalopathy (SAE) is a common but serious complication in septic survivors and often causes long‐term cognitive impairments. The role of RIPK3‐participated necroptosis in SAE remains obscured. STING is a key molecule in regulating necroptosis and apoptosis. However, there is uncertainty as to the mechanisms of STING in CLP‐induced SAE. The aim of this study was to investigate whether STING is involved in the underlying mechanism of SAE.MethodsThe contextual fear conditioning test (CFCT) assesses cognitive impairment. A transmission electron microscope (TEM) was used to notice the necroptosis. Western blotting and immunofluorescence labeling were applied for the observation of related proteins.ResultsThe phosphorylated STING in the hippocampal neuron of SAE mice was significantly elevated. Knocking down STING inhibited necroptosis and attenuated cognitive impairment in SAE mice. Moreover, RIPK3−/− mice had less cognitive deficit in the SAE model. However, STING overexpression did not deteriorate cognitive impairment in RIPK3−/− mice with SAE, indicating that STING is upstream involved in necroptosis. Furthermore, PERK inhibition ameliorated cognitive deficits through a STING‐dependent pathway in SAE mice.ConclusionPERK‐STING‐RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in the pathology of SAE, which provided a new therapeutic target in SAE treatment.