bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strong aftershocks traffic light system: A case study of the 8 January 2022 MS6.9 Menyuan earthquake, Qinghai Province, China
Authors: Bi Jinmeng , Yin Fengling , Jiang Changsheng , Yin Xinxin , Ma Yong , Song Cheng 
Published: January 2023

Strong aftershocks, especially the disaster-causing M≥5.0 kind, are a key concern for mitigation of seismic risks because they often lead to superimposed earthquake damage. However, the real-time forecasting results of the traditional probability prediction models based on statistics are usually far from accurate and therefore unsatisfactory. Borrowing an idea from the foreshock traffic light system (FTLS), which is based on observations of decreasing b-values or increasing differential stress just before a strong aftershock, we constructed a strong aftershock traffic light system (SATLS) that uses data-driven technology to improve the reliability of time sequence b-value calculations, and analyzed the b-value variations of strong aftershocks in the China continent. We applied this system to the MS6.9 Menyuan earthquake occurred on 8 January 2022. The earthquake occurrence rates before the largest aftershock (MS5.2) forecast by the Omi-R-J model were too low, although the model could accurately forecast aftershock rates for each magnitude interval in most time-periods. However, reliable b-values can be calculated using the time-sequence b-value data-driven (TbDD) method, and the results showed that the b-values continued declining from 1.3 days before the MS5.2 aftershock and gradually recovered afterward. This would suggest that the stress evolution in the focal area can provide data for deciding when to post risk alerts of strong aftershocks. In the process of building the SATLS, we studied thirty-four M≥6.0 intraplate earthquake sequences in the China continent and concluded that the differences between the b-values of the aftershock sequences and of the background events, △b = bafter - bbg = ±0.1, could be used as thresholds to determine whether M≥5.0 aftershocks would occur. The △b value obtained using the events before the MS5.2 aftershock of the MS6.9 Menyuan sequence was about -0.04, which would have caused the SATLS to declare a yellow alert, but there would have been some gap expected before a red alert was triggered by the b-value difference derived from the events associated with this strong aftershock. To accurately forecast a strong aftershock of M≥5.0, a deeper understanding of the true b-value and a detailed description of the stress evolution state in the source area is necessary.
Elevated visceral adiposity index is associated with increased stroke prevalence and earlier age at first stroke onset: Based on a national cross-sectional study
Authors: Chen Qingjie , Zhang Ziwen , Luo Ning , Qi Yilong 
Published: January 2023

ObjectiveThe purpose of this study was to examine the association between the VAI (visceral adiposity index) and stroke prevalence and age at stroke in US adults.MethodsWe examined the association between VAI and stroke prevalence and age at stroke using logistic regression, subgroup analysis, and dose-response curves using participants from the National Health and Nutrition Examination Survey (NHANES) database from 2007-2018.ResultsThis study ultimately included 29,337 participants aged &gt;20 years, of whom 1022 self-reported a history of stroke, and after adjusting for all confounders, each unit increase in corrected VAI was associated with a 12% increase in the prevalence of stroke (OR= 1.12, 95% CI: 1.01, 1.24) along with an earlier age at stroke 1.64 years (β= -1.64, 95% CI: -2.84, -0.45), stratified analysis showed that the prevalence of stroke was 20% higher in the female group (OR= 1.20, 95% CI: 1.04, 1.39), black group (OR= 1.22, 95% CI: 1.01, 1.48), age ≤60 years group (OR= 1.25, 95% CI: 1.05, 1.48), hypertensive group (OR=1.15, 95% CI:1.01, 1.31), and diabetic group (OR=1.23, 95% CI:1.02, 1.48) VAI increase was positively correlated with stroke prevalence increase. The dose-response curves showed a positive linear correlation between increased VAI and stroke prevalence, while a negative linear correlation was observed between increased VAI and age at stroke.ConclusionAlthough a causal relationship cannot be proven, higher VAI was positively associated with stroke prevalence and can lead to earlier stroke onset.
Field verification of low-level biochar applications as effective ameliorants to mitigate cadmium accumulation into Brassica campestris L from polluted soils
Authors: Zhang Youchi , Liu Shuang , Lin Shanna , Reid Brian J. , Coulon Frederic , Cai Chao 
Published: January 2023

Introduction: Cadmium (Cd) has been recognized as a significant contributor to the pollution of farmland soils in China, and biochars have been reported to be effective in mitigating soil Cd pollution. However, most studies have been conducted in laboratory or greenhouse settings, not at a field scale, and the biochars used have been applied at unrealistically high amounts (&gt;10 t/ha).Methods: In this research, three biochars: rice straw biochar (RSB), pig manure biochar (PMB) and rice husk biochar (RHB) were produced from readily available farm residues. Then the effects at low-level application (1.8 and 3.6 t/ha) on Cd were investigated in a field experiment cropped with rape (Brassica campestris L.).Results: Batch adsorption experiments indicated Cd adsorption capacity of three biochars followed the order of RSB (43.5 mg/g) &gt; PMB (33.3 mg/g) &gt; RHB (24.4 mg/g). Field experiment indicated biochar amendments could slightly change soil pH and cation exchange capacity (CEC); yet led to considerable and significant decreases in extractable Cd concentrations [reductions of: 43%–51% (PMB), 29%–35% (RSB) and 17%–19% (RHB)]. Reduced extractable Cd correlated with lower Cd concentrations in rape plants. PMB and RSB were more effective in decreasing Cd phytoaccumulation into edible parts of rape (&gt;68% reduction) than RHB.Discussion: Low-level application of PMB or RSB could efficiently decrease the phytoaccumulation of Cd from soils into crops. These results demonstrate the reality of biochar-based remediation solutions to contribute to the mitigation of diffuse Cd contamination in farmland. The results also highlight the need to trail biochars in the presence of the soil to be targeted for remediation.
Short-term land degradation driven by livestock grazing does not affect soil properties in semiarid steppe rangelands
Authors: Macheroum Amale , Chenchouni Haroun 
Published: January 2023

The soil–vegetation relationships are reciprocal and fundamental for terrestrial ecosystem integrity. However, the long-term degradation of vegetation cover alters edaphic conditions, which can lead to degradation of habitats, and obstructs proper ecosystem functioning. This study aimed to assess the effects of the degradation of perennial steppe vegetation due to overgrazing (i.e., continuous and free grazing) on the physicochemical characteristics and soil fertility in the steppe rangelands of Halfa (Macrochloa tenacissima syn. Stipa tenacissima) of semi-arid areas in Algeria. The edaphic parameters of the superficial horizons of degraded steppes and other undegraded steppes were compared in order to suggest adequate strategies for rangeland management and remedy the degradation of vegetation and thereby ensure the sustainability of these agro-pastoral systems. The soil, collected from the surface horizons (A1 and A2) of pedological profiles, was analyzed according to standard methods of soil physicochemical analyses to determine the particle size fractions (clay, silt, and sands), pH, electrical conductivity (EC), total and active CaCO3, organic matter (SOM), organic carbon, total nitrogen, and the C:N ratio. The distribution and variation of soil parameters between horizons and the two types of steppes were examined using generalized linear mixed models and redundancy analysis. Findings of this study revealed that the degradation of steppe vegetation cover by short-term overgrazing did not cause a significant variability in soil physicochemical parameters between degraded and undegraded steppes and the horizons A1 and A2. The sites studied have clayey–silty textures in non-degraded steppes and coarse textures dominated by sands in degraded steppes. The edaphic environment of the Halfa grass steppes was characterized by a calcareous substratum with high carbonate content (total CaCO3 = 36.8–41%, active CaCO3 = 17.5–18.5%), with an alkaline pH (8.09–8.19) and EC averaging 0.99 ± 1.24 dS/m. Soils had low SOM contents (1.42–2.93%), organic carbon (0.82–1.64%), and nitrogen (0.1–0.15%). The strong positive correlations recorded between the fine-size fractions, SOM, total CaCO3, nitrogen, and EC indicated good soil structural stability in undegraded steppes, while the negative correlations between these same variables with sand and pH substantiate the structural degradation of the soil. The negative correlation between C:N ratio and SOM indicated that the rate of SOM mineralization was rapid in semiarid steppes, with a higher fertility state in the undegraded steppe. Our findings suggest and urge to implement a restoration plan against the degradation of semi-arid Halfa steppes in order to avoid the irreversible state of soil and habitat destruction in the future because even the degradation of rangeland plant cover due to livestock overgrazing—in the short term—has not affected the edaphic characteristics of steppe rangelands.
Myopia and its associated factors among pregnant women at health institutions in Gondar District, Northwest Ethiopia: A multi-center cross-sectional study
Authors: Diress Mengistie , Getnet Mihret , Akalu Yonas , Dagnew Baye , Belsti Yitayeh , Gela Yibeltal Yismaw , Chilot Dagmawi , Belay Daniel Gashaneh , Bitew Desalegn Anmut , Terefe Bewuketu , Getahun Amare Belete 
Published: January 2023

BackgroundMyopia is the most common form of uncorrected refractive error with a growing burden worldwide. It is the principal complaint of most women during pregnancy. Although myopia has led to several consequences across the standard life of pregnant women, there is no previous study in Ethiopia regarding this topic. Thus, this study determined the prevalence of myopia and identifies its associated factors among pregnant women attending antenatal care units at governmental health institutions in Gondar City District, Northwest Ethiopia.MethodsAn institution-based cross-sectional study design was conducted from 08 February to 08 April 2021. From the selected health centres, study participants were recruited by systematic random sampling technique. A pre-tested, structured-interviewer-administered questionnaire consisting of socio-demographic variables, obstetric and clinical-related variables was used to collect the required data. Non-cycloplegic refraction was performed using trial lenses, trial frames, and retinoscopy in a semi-dark examination room. EpiData 3 and STATA 14 were used for data entry and statistical analysis respectively. Both bivariable and multivariable binary logistic regression analyses were executed to identify associated factors of myopia. Variables with a p-value ≤0.05 in the multivariable logistic regression analysis were declared as statistically significant with myopia. Model fitness was checked by Hosmer and Lemeshow goodness of test (at p &gt; 0.05)ResultsA total of four-hundred and twenty-three pregnant women participated with a 100% response rate in this study. The overall prevalence of myopia among pregnant women was 26.48% (95% CI: 22.48–30.91). Eighty-Eight (20.81%) and Eighty-Four (19.85%) of the study participants had myopia in their right and left eyes respectively. The prevalence of myopia was significantly associated with age (AOR = 1.17; 95% CI: 1.09–1.28), the third trimester of gestation (AOR = 2.05, 95% CI: 1.08–3.90), multi &amp; grand multipara (AOR = 3.15; 95% CI: 1.59–6.25), and history of contraceptive use (AOR = 3.30; 95% CI: 1. 50–7.28).ConclusionThe finding of our study shows that there is a higher prevalence of myopia among pregnant women in our study area. Further prospective analytical studies regarding visual systems among pregnant women, particularly as a result of pregnancy, are strongly recommended.
Exploring the causality and pathogenesis of systemic lupus erythematosus in breast cancer based on Mendelian randomization and transcriptome data analyses
Authors: Li Wenjie , Wang Rong , Wang Wei 
Published: January 2023

IntroductionThere has been a cumulative interest in relationships between systemic lupus erythematosus (SLE) and cancer risk. Breast cancer is the most common cancer among women worldwide. However, the casual association and pathogenesis between SLE and breast cancer remains incompletely unknown.MethodsMendelian randomization (MR) analysis was first conducted to investigate the potential causality between SLE and breast cancer. Sensitivity analyses were applied to validate the reliability of MR results. Transcriptomic data analyses based on the Cancer Genome Atlas and Gene Expression Omnibus databases were then performed to identify and construct a SLE-related gene signature (SLEscore).ResultsThe MR analysis demonstrated that genetic predisposition to SLE was casually associated with the decreased risk of breast cancer in the East Asian cohort (odds ratios: 0.95, 95% confidence interval: 0.92-0.98, p=0.006). However, no casual associations were observed in the European population. Furthermore, sensitivity analyses proved the robustness of the present MR results. A prognostic SLEscore consisting of five SLE-related genes (RACGAP1, HMMR, TTK, TOP2A, and KIF15) could distribute patients with breast cancer into the high- and low-risk groups according to survival rates with good predictive ability (p &lt; 0.05).ConclusionOur MR study provided evidence that genetic changes in SLE were significantly associated with the decreased risk of breast cancer in the East Asian population, while no causality was found in the European cohorts. Transcriptome data analyses indicated that the SLEscore could serve as a novel biomarker for predicting prognosis when breast cancer and SLE coexisted in patients.
Virotherapy combined with anti-PD-1 transiently reshapes the tumor immune environment and induces anti-tumor immunity in a preclinical PDAC model
Authors: Veinalde Rūta , Pidelaserra-Martí Gemma , Moulin Coline , Tan Chin Leng , Schäfer Theresa E. , Kang Na , Ball Claudia R. , Leichsenring Jonas , Stenzinger Albrecht , Kaderali Lars , Jäger Dirk , Ungerechts Guy , Engeland Christine E. 
Published: January 2023

IntroductionPancreatic ductal adenocarcinoma (PDAC) is largely refractory to cancer immunotherapy with PD-1 immune checkpoint blockade (ICB). Oncolytic virotherapy has been shown to synergize with ICB. In this work, we investigated the combination of anti-PD-1 and oncolytic measles vaccine in an immunocompetent transplantable PDAC mouse model.MethodsWe characterized tumor-infiltrating T cells by immunohistochemistry, flow cytometry and T cell receptor sequencing. Further, we performed gene expression profiling of tumor samples at baseline, after treatment, and when tumors progressed. Moreover, we analyzed systemic anti-tumor and anti-viral immunity.ResultsCombination treatment significantly prolonged survival compared to monotherapies. Tumor-infiltrating immune cells were increased after virotherapy. Gene expression profiling revealed a unique, but transient signature of immune activation after combination treatment. However, systemic anti-tumor immunity was induced by virotherapy and remained detectable even when tumors progressed. Anti-PD-1 treatment did not impact anti-viral immunity.DiscussionOur results indicate that combined virotherapy and ICB induces anti-tumor immunity and reshapes the tumor immune environment. However, further refinement of this approach may be required to develop its full potential and achieve durable efficacy.
Inflammatory and deleterious role of gut microbiota-derived trimethylamine on colon cells
Authors: Jalandra Rekha , Makharia Govind K. , Sharma Minakshi , Kumar Anil 
Published: January 2023

Trimethylamine (TMA) is produced by the intestinal microbiota as a by-product of metabolism of dietary precursors. TMA has been implicated in various chronic health conditions. However, the effect of TMA in the colon and the underlying mechanism was not clear. In this study, TMA exhibited toxic effects in vitro as well as in vivo. TMA-induced oxidative stress causes DNA damage, and compromised cell membrane integrity leading to the release of LDH outside the cells which ultimately leads to cell death. Besides, TMA also exhibited pronounced increase in cell cycle arrest at G2/M phase in both HCT116 and HT29 cell lines. TMA was found to be genotoxic and cytotoxic as the TMA concentration increased from 0.15 mM. A decreased ATP intracellular content was observed after 24 h, 48 h, and 72 h treatment in a time and dose-dependent manner. For in vivo research, TMA (100 mM, i.p. and intra-rectal) once a week for 12 weeks caused significant changes in cellular morphology of colon and rectum epithelium as assessed by H &amp; E staining. TMA also significantly increased the infiltration of inflammatory cells in the colon and rectal epithelium indicating the severity of inflammation. In addition, TMA caused extensive mucosal damage and distortion in the epithelium, decrease in length of small intestine compared to control mice. In conclusion, these results highlight the detrimental effects of TMA in the colon and rectal epithelium.
The efficacy and safety of lebrikizumab monotherapy for the management of moderate-to-severe atopic dermatitis: A systematic review and meta-analysis
Authors: Bashrahil Bader , Alzahrani Ziyad , Samarkandy Sahal , Aman Abdullah , Jfri Abdulhadi 
Published: January 2023

BackgroundAtopic dermatitis (AD) is a chronically relapsing disease. Few biologics are approved for moderate-to-severe AD, and novel interventions are emerging. We aimed to evaluate the safety and efficacy of lebrikizumab, an IL-13 immunomodulator, as monotherapy vs. placebo in treating moderate-to-severe AD.MethodsCochrane Central Register of Controlled Trials (CENTRAL), Medline, Embase, and ClinicalTrials.gov registry (CT.gov) databases were systematically searched. We evaluated lebrikizumab vs. placebo and measured efficacy using Eczema Area and Severity Index (EASI), Body Surface Area (BSA), and Investigator’s Global Assessment (IGA) change from baseline to week 16. Safety was evaluated by the incidence of serious adverse events (SAEs), non-serious adverse events (NSAEs), and mortality. The risk of bias was investigated using the Revised Cochrane risk of bias tool.ResultsThree RCTs (n = 1,149) included 543 (47.25%) men vs. 606 (52.75%) women. Meta-analysis showed statistically significant improvement in EASI, IGA, and BSA. EASI75 at week 16 for all regimens was (RR = 2.62, 95% CI [2.06, 3.34], p &lt; 0.00001) with the first regimen (500 mg loading dose then 200 mg every 2 weeks) showing the most significant improvement (RR = 3.02, 95% CI [2.39, 3.82], p &lt; 0.00001). The pooled analysis of safety outcomes concluded that lebrikizumab did not correlate significantly with the incidence of SAEs, NSAEs, and mortality.ConclusionOverall, lebrikizumab showed a significant improvement in all efficacy outcomes. Additionally, it did not contribute to any significant incidence of SAEs, NSAEs, or mortality. The risk of bias in included RCTs was minor except in the randomization domain. Grading of Recommendations Assessment, Development, and Evaluation (GRADE) assessment of the outcomes ranged from low to high, but predominantly high certainty of evidence.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022362438.
Whole-genome sequencing reveals genomic characterization of Listeria monocytogenes from food in China
Authors: Ji Shunshi , Song Zexuan , Luo Lijuan , Wang Yiqian , Li Lingling , Mao Pan , Ye Changyun , Wang Yan 
Published: January 2023

IntroductionListeria monocytogenes is a foodborne bacterium that could persist in food and food processing environments for a long time. Understanding the population structure and genomic characterization of foodborne L. monocytogenes is essential for the prevention and control of listeriosis.MethodsA total of 322 foodborne L. monocytogenes isolates from 13 geographical locations and four food sources in China between 2000 and 2018 were selected for whole-genome sequencing.ResultsIn silico subtyping divided the 322 isolates into five serogroups, 35 sequence types (STs), 26 clonal complexes (CCs) and four lineages. Serogroup IIa was the most prevalent serogroup and ST9 was the most prevalent ST of foodborne L. monocytogenes strains isolated in China. The in-depth phylogenetic analysis on CC9 revealed that ST122 clone might be original from ST9 clone. Furthermore, 23 potentially relevant clusters were identified by pair-wised whole-genome single nucleotide polymorphism analysis, indicating that persistent- and/or cross-contamination had occurred in markets in China. ST8 and ST121 were the second and third top STs of L. monocytogenes in China, which had heterogeneity with that of L. monocytogenes isolates from other countries. The antibiotic resistance genes aacA4, tetM, tetS, dfrG carried by different mobile elements were found in L. monocytogenes strains. One lineage II strain carrying Listeria Pathogenicity Island 3 was first reported. In addition, a novel type of premature stop codon in inlA gene was identified in this study.DiscussionThese findings revealed the genomic characteristics and evolutionary relationship of foodborne L. monocytogenes in China on a scale larger than previous studies, which further confirmed that whole-genome sequencing analysis would be a helpful tool for routine surveillance and source-tracing investigation.
Effect of olive by-products feed supplementation on physicochemical and microbiological profile of Provola cheese
Authors: Calabrese Francesco Maria , Russo Nunziatina , Celano Giuseppe , Pino Alessandra , Lopreiato Vincenzo , Litrenta Federica , Di Bella Giuseppa , Liotta Luigi , De Angelis Maria , Caggia Cinzia , Randazzo Cinzia L. 
Published: January 2023

IntroductionWith the purpose to evaluate the effects of dietary olive cake, a source of bioactive phenolic compounds, as feed supplementation of lactating dairy cows on fatty acid composition, volatile organic compounds, and microbiological profiles of Provola cheese, we performed a two-arm study where control and experimental administered cows derived dairy have been compared.MethodsOur panel of analyses include metabolomics, physicochemical detected variables, culture dependent and independent analyses, and a stringent statistical approach aimful at disclosing only statistically significant results.Results and discussionLooking at the physicochemical variable’s profiles, a higher content of unsaturated fatty acids, polyunsaturated fatty acid, and conjugated linoleic acids as well of proteins were observed in experimental cheese samples, indicating the beneficial effect of dietary supplementation. Furthermore, based on volatilome composition, a clear cluster separation between control and experimental cheeses was obtained, mainly related to terpenes degradation, able of influencing their aroma and taste. Microbiological results showed a decrease of some spoilage related microbial groups in experimental cheeses, probably due to the inhibitory effect exerted by polyphenols compounds, that contrarily did not affect the core taxa of all cheese samples. This paper confirmed the promising utilization of olive by-product in farming practices to obtain more sustainable and safe dairy food products with lower environmental impact, mainly in Sicily and Mediterranean area, where waste disposal poses serious environmental and economic problems.