bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automatic lumen and anatomical layers segmentation in IVOCT images using meta learning
Authors: Shi Peiwen , Xin Jingmin , Du Shaoyi , Wu Jiayi , Deng Yangyang , Cai Zhuotong , Zheng Nanning 
Published: June 2023

AbstractAutomated analysis of the vessel structure in intravascular optical coherence tomography (IVOCT) images is critical to assess the health status of vessels and monitor coronary artery disease progression. However, deep learning‐based methods usually require well‐annotated large datasets, which are difficult to obtain in the field of medical image analysis. Hence, an automatic layers segmentation method based on meta‐learning was proposed, which can simultaneously extract the surfaces of the lumen, intima, media, and adventitia using a handful of annotated samples. Specifically, we leverage a bi‐level gradient strategy to train a meta‐learner for capturing the shared meta‐knowledge among different anatomical layers and quickly adapting to unknown anatomical layers. Then, a Claw‐type network and a contrast consistency loss were designed to better learn the meta‐knowledge according to the characteristic of annotation of the lumen and anatomical layers. Experimental results on the two cardiovascular IVOCT datasets show that the proposed method achieved state‐of‐art performance.
Determination of potential combination of non‐β‐lactam, β‐lactam, and β‐lactamase inhibitors/β‐lactam enhancer against class D oxacillinases producing Acinetobacter baumannii: Evidence from in‐vitro, molecular docking and dynamics simulation
Authors: Gopikrishnan Mohanraj , Ramireddy Sriroopreddy , Varghese Rinku Polachirakkal , Bakathavatchalam Yamuna Devi , D Thirumal Kumar , Manesh Abi , Walia Kamini , Veeraraghavan Balaji , C George Priya Doss 
Published: June 2023

AbstractCarbapenem‐resistant Acinetobacter baumannii, a predominant nosocomial pathogen in hospitals of intensive care units, is associated with bacteremia and ventilator‐associated pneumonia with a high‐risk mortality rate. To increase the effectiveness of the β‐lactam (BL) antibiotics, the use of β‐lactamase inhibitors (BLI) acts as a booster when given in combination with BL antibiotics. To this aspect, we selected BL antibiotics of cefiderocol, cefepime, non‐BL antibiotic eravacycline, BLI of durlobactam, avibactam, and a β‐lactam enhancer (BLE) of zidebactam. To prove our hypothesis, we determined the minimum inhibitory concentration (MIC) of various BL or non‐BL/BLI or BLE combinations using broth microdilution method followed by in silico analysis of molecular docking, molecular dynamics (MD) simulation, and molecular mechanics Poisson–Boltzmann surface area (MM‐PBSA) identifies the potential combination. In MIC testing, eravacycline, cefepime/zidebactam, cefiderocol/zidebactam, and eravacycline in combination with zidebactam or durlobactam were found to be effective against oxacillinases (OXAs) (OXA‐23/24/58 like) expressing A. baumannii isolates. The docking results of the selected ligands toward OXA‐23, OXA‐24, and OXA‐58 had an excellent binding score ranging from −5.8 to −9.3 kcal/mol. Further, the docked complexes were subjected and evaluated using gromacs for molecular dynamics simulation of 50 ns toward selected class D OXAs. The binding energies obtained from MM‐PBSA shed light on the binding efficiencies of each non‐BL, BL, and BLI/BLE, thereby helping us to propose the drug combinations. Based on the MD trajectories scoring acquired, we propose using eravacycline, cefepime/zidebactam, cefiderocol/zidebactam, and eravacycline in combination with durlobactam or zidebactam would be promising for treating OXA‐23, OXA‐24, and OXA‐58 like expressing A. baumannii infections.
Selective [3 + 2] cycloaddition reaction of isothiazol‐3(2h)‐ones with in situ formed azomethine ylide to thiazolidines and oxazolidines
Authors: Jin Wen Bin , Wang Zhen , Yang Wei , Zhang Die , Ning Jin Hua , Ke Jing , Hou An Guo , Chen Lin Yun , Ma Yun Shu 
Published: June 2023

AbstractNovel selective [3 + 2] cycloaddition reactions between in situ formed nonstabilized azomethine ylide generated from N‐benzyl‐1‐methoxy‐N‐((trimethylsilyl)methyl)methanamine enabled by Lewis acids and bioactive molecules isothiazol‐3(2H)‐ones have been developed. The reaction selectively afforded novel thiazolidines or cyclo‐fused oxazolidinones depending on Lewis acid catalyst system of reaction used.
Profiling the acute phase antibody response against mpox virus in patients infected during the 2022 outbreak
Authors: Colavita Francesca , Matusali Giulia , Mazzotta Valentina , Bettini Aurora , Lapa Daniele , Meschi Silvia , Francalancia Massimo , Pinnetti Carmela , Bordi Licia , Mizzoni Klizia , Coen Sabrina , Girardi Enrico , Vaia Francesco , Nicastri Emanuele , Antinori Andrea , Maggi Fabrizio 
Published: June 2023

AbstractInformation on the immune response during the mpox virus (MPXV) infection is still scarce or limited to past studies when cross‐reactive immunity from smallpox vaccination was predominant. Here, we describe the short‐term kinetics of the antibody response in patients with acute MPXV infection during the 2022 multicountry outbreak. A total of 64 samples from 18 MPXV‐positive patients were longitudinally collected from the day of symptom onset (DSO) up to 20 days after and tested for anti‐MPXV immunoglobulin G (IgG), IgM, IgA, and neutralizing antibodies (nAb) using the whole‐live virus isolated in May 2022. IgG, IgM, and IgA were detected as early as 4 DSO (median time of seroconversion 7.5 DSO for IgG, 8 DSO for IgM and IgA). Anti‐MPXV nAb were detectable in samples collected as early as 1 week after symptoms, with stable levels up to 20 DSO. After 2 weeks, IgG and nAb reached high titers. No significant differences were observed regardless of status of smallpox vaccination, human immunodeficiency virus positivity, or disease severity. Significant lower levels of IgM and IgG were observed in the patients treated with antivirals. These results contribute to extending the knowledge of the MPXV infection and the antibody response in a population with no historic smallpox vaccination.
Magnetic Resonancy Imaging Safety in Active Osseointegrated (Osia®) and Cochlear Implants: New Technology Creating Confusion
Authors: Abdalla Ibrahim , Choi Janet S. , Struyk Griffin , Adams Meredith E. , Huang Tina C. 
Published: June 2023

ObjectiveThere is increased confusion regarding MRI‐compatible CIs and BAHAs. This report describes two cases when patients underwent MRIs with non‐MRI compatible devices.ResultsOne patient with bilateral Cochlear Osias experienced dislocation of both internal magnets after 1.5 Tesla MRI. Both magnets were outside the silastic sheath, with the left magnet flipped. A second patient with a legacy CI experienced similar internal magnet dislocation and inversion after 3 Tesla MRI.ConclusionsThis study describes internal magnet dislocation/inversion with the Cochlear Osia and a legacy CI after MRI. Our findings suggest the need for improved patient education and simplified radiology guidelines. Laryngoscope, 134:393–396, 2024
Quantitatively distinguishing the impact of solar photovoltaics programs on vegetation in dryland using satellite imagery
Authors: Xia Zilong , Li Yingjie , Zhang Wei , Guo Shanchuan , Zheng Lilin , Jia Nan , Chen Ruishan , Guo Xiaona , Du Peijun 
Published: June 2023

AbstractGlobal drylands are experiencing booming development of centralized photovoltaics (PV), which aims to address the dual challenges posed by climate change and energy transformation. In dryland areas with large‐scale deployment of solar PV infrastructure, vegetation was reported to experience drastic changes. However, the long‐term dynamic changes and driving mechanisms have not been thoroughly studied yet. Quantitatively distinguishing the disturbances of climate change and PV plant deployment on vegetation change is the key to understanding the environmental impact of clean energy development and formulating adaptive ecological recovery measures. To understand this, we selected the Gonghe solar power project in northern China, one of the largest dryland PV plants in the world, as a case study. Specifically, satellite‐derived Normalized Difference Vegetation Index (NDVI) and meteorological data from ground stations were used to analyze the changing patterns of vegetation growth and climatic factors. The relative contributions of climatic factors and PV plant deployment to NDVI change were quantified by multiple regression analysis. The results indicated that vegetation has increased gradually since 2000, with the rate of vegetation recovery doubled during the PV expansion period. From 2013 to 2020, climate change was the main driver of increased vegetation (56%), followed by the expansion of solar PV infrastructures (44%). Vegetation inside PV arrays increased 1.4 times faster than outside, mainly because the PV panels improve the efficiency of rainwater utilization in summer and reduce the negative impact of excessive sunlight in the growing season. In addition, vegetation management practices like grazing can further enhance carbon sequestration and create sustainable livelihood opportunities, achieving sustainable economic, social, and ecological development. The novelty of the study lies in the proposed framework to quantify the impact of solar PV programs on vegetation in dryland allowing easy interpretations of vegetation dynamics under clean energy development and climate change, which provide scientific references for clean energy planning and ecological recovery in arid areas.
Forage accumulation and nutritive value of four peanut (Arachis hypogaea L.) market types in the US Southern Great Plains
Authors: Witt Travis , Northup Brian , Ojha Manisha , Puppala Naveen 
Published: June 2023

AbstractDual use crops are becoming popular as resources like land and water become scarcer. Annual peanuts are an important food crop grown worldwide that can also be used as forage or hay. Replicated trials evaluated the late season (≥15 weeks after planting) forage accumulations and nutritive values (acid detergent fiber, carbon content, crude protein, invitro true digestibility, neutral detergent fiber, nitrogen content, and relative feed value) of four market types near Clovis, NM and El Reno, OK in 2019 and 2020. Statistical analyses (p ≤ .05) showed all traits were affected by the interaction between market type and growing environment. Overall, forage accumulations for all market types ranged from 2.7 to 6.5 Mg ha−1 with relative feed values of 107–155. The location average across sites was 4.45 Mg ha−1 with relative feed value of 132. The Virginia market types produced large amounts of biomass. However, the Valencia market type generated the greatest nutritive values. This study indicates harvesting late‐season biomass of peanuts for hay may be an option that allows producers to generate more income from limited land and water resources. However, both market type and environmental factors, such as rainfall/irrigation, play important roles in production and forage nutritive values of peanut forage.
Overcoming the blockchain technology credibility gap
Authors: Mazzù Marco Francesco , Pozharliev Rumen , Andria Alberto , Baccelloni Angelo 
Published: June 2023

AbstractBlockchain technology has been designed to improve the transmission of transparent information across a variety of industries and products. Yet, consumers tend to perceive product information provided by blockchain technology (vs. humans) as less credible. As this may not apply to all consumers, it becomes critical for companies to understand how to improve blockchain perceived credibility. This work investigates how individual differences and marketing actions shape consumer responses to product information provided by blockchain technology (vs. humans). Four controlled experiments demonstrate that consumers perceive the information provided by blockchain technology (vs. humans) as having less credibility, which in turn decreases word‐of‐mouth and intention to share information about the product on social media (Study 1). This effect is stronger for consumers with lower need for cognition (Study 2a), which in turn affects willingness to buy and actual behavior (Study 2b). Providing social proof—that is, the number of satisfied customers who recommend blockchain technology—increases blockchain perceived credibility (Study 3). These insights deepen the understanding of how individual differences shape consumer's responses to product information provided by blockchain technology and offer actionable insights on how to boost technology credibility.
Southern Flounder Life History Diversity and Contributions to Fisheries from Differing Estuarine Salinity Zones
Authors: Chrisp Jared K. , Nelson T. Reid , Sackett Dana K. , Farmer Troy M. 
Published: June 2023

Abstract
Otolith chemistry is a useful natural tracer for discerning habitat-use of estuarine fishes. For Southern Flounder Paralichthys lethostigma, recent otolith chemistry studies have revealed a diversity of residency patterns across salinity gradients. However, the contribution of recruits with specific residency patterns to fisheries is poorly understood. The objectives of this study were to (1) use otolith chemistry from fishery-independent and fishery-dependent collections in Mobile Bay, Alabama, to classify lifetime residency patterns (i.e., freshwater, transient, estuarine) in Southern Flounder collected across a large estuarine salinity gradient (0–30 psu); (2) to test if Southern Flounder exhibited resident or migratory behavior by determining if lifetime residency patterns in fishery-independent samples matched expected salinity patterns in the region of collection after accounting for annual variation in river discharge; and (3) to examine which residency patterns contributed to the commercial and recreational Southern Flounder fisheries in nearby coastal waters. Age-0 residency patterns in fishery-independent samples were strongly correlated with region of collection and annual river discharge, suggesting that the majority of Southern Flounder had resided in the same region in which they spent their age-0 growth phase. Southern Flounder with a combination of freshwater and estuarine salinity signals and classified as “transient” did not appear to have conducted large-scale movements across salinity gradients, but instead resided in regions of the estuary experiencing seasonal fluctuations in salinity. The majority (57%) of commercially and recreationally harvested Southern Flounder were transients, while a minority (39%) were estuarine residents and lifetime freshwater residents (4%) were rarely harvested. Results from this study suggest that Southern Flounder settle in and remain in the certain habitats during the estuarine residency phase. Given the lack of movement across habitats, future efforts to understand how habitat-specific conditions (e.g., abiotic, biotic, fishing exploitation) affect vital rates seems warranted for a species currently experiencing population declines.
Spinocerebellar Ataxia Type 1 Characteristics in Patient‐Derived Fibroblast and iPSC‐Derived Neuronal Cultures
Authors: Buijsen Ronald A.M. , Hu Michel , Sáez‐González Maria , Notopoulou Sofia , Mina Eleni , Koning Winette , Gardiner Sarah L. , van der Graaf Linda M. , Daoutsali Elena , Pepers Barry A. , Mei Hailiang , van Dis Vera , Frimat Jean‐Philippe , van den Maagdenberg Arn M. J. M. , Petrakis Spyros , van Roon‐Mom Willeke M.C. 
Published: June 2023

AbstractBackgroundSpinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disease caused by a polyglutamine expansion in the ataxin‐1 protein resulting in neuropathology including mutant ataxin‐1 protein aggregation, aberrant neurodevelopment, and mitochondrial dysfunction.ObjectivesIdentify SCA1‐relevant phenotypes in patient‐specific fibroblasts and SCA1 induced pluripotent stem cells (iPSCs) neuronal cultures.MethodsSCA1 iPSCs were generated and differentiated into neuronal cultures. Protein aggregation and neuronal morphology were evaluated using fluorescent microscopy. Mitochondrial respiration was measured using the Seahorse Analyzer. The multi‐electrode array (MEA) was used to identify network activity. Finally, gene expression changes were studied using RNA‐seq to identify disease‐specific mechanisms.ResultsBioenergetics deficits in patient‐derived fibroblasts and SCA1 neuronal cultures showed altered oxygen consumption rate, suggesting involvement of mitochondrial dysfunction in SCA1. In SCA1 hiPSC‐derived neuronal cells, nuclear and cytoplasmic aggregates were identified similar in localization as aggregates in SCA1 postmortem brain tissue. SCA1 hiPSC‐derived neuronal cells showed reduced dendrite length and number of branching points while MEA recordings identified delayed development in network activity in SCA1 hiPSC‐derived neuronal cells. Transcriptome analysis identified 1050 differentially expressed genes in SCA1 hiPSC‐derived neuronal cells associated with synapse organization and neuron projection guidance, where a subgroup of 151 genes was highly associated with SCA1 phenotypes and linked to SCA1 relevant signaling pathways.ConclusionsPatient‐derived cells recapitulate key pathological features of SCA1 pathogenesis providing a valuable tool for the identification of novel disease‐specific processes. This model can be used for high throughput screenings to identify compounds, which may prevent or rescue neurodegeneration in this devastating disease. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Identification and functional analysis of a novel de novo missense mutation located in the initiation codon of LAMP2 associated with early onset female Danon disease
Authors: Wang Yongxiang , Bai Ming , Zhang Piyi , Peng Yu , Chen Zixian , He Zhiyu , Xu Jin , Zhu Youqi , Yan Dongdong , Wang Runqing , Zhang Zheng 
Published: June 2023

AbstractBackgroundDanon disease is characterized by the failure of lysosomal biogenesis, maturation, and function due to a deficiency of lysosomal membrane structural protein (LAMP2).MethodsThe current report describes a female patient with a sudden syncope and hypertrophic cardiomyopathy phenotype. We identified the pathogenic mutations in patients by whole‐exon sequencing, followed by a series of molecular biology and genetic approaches to identify and functional analysis of the mutations.ResultsSuggestive findings by cardiac magnetic resonance (CMR), electrocardiogram (ECG), and laboratory examination suggested Danon disease which was confirmed by genetic testing. The patient carried a novel de novo mutation, LAMP2 c.2T>C located at the initiation codon. The quantitative polymerase chain reaction (qPCR) and Western blot (WB) analysis of peripheral blood leukocytes from the patients revealed evidence of LAMP2 haploinsufficiency. Labeling of the new initiation codon predicted by the software with green fluorescent protein followed by fluorescence microscopy and Western blotting showed that the first ATG downstream from the original initiation codon became the new translational initiation codon. The three‐dimensional structure of the mutated protein predicted by alphafold2 revealed that it consisted of only six amino acids and failed to form a functional polypeptide or protein. Overexpression of the mutated LAMP2 c.2T>C showed a loss of function of the protein, as assessed by the dual‐fluorescence autophagy indicator system. The mutation was confirmed to be null, AR experiments and sequencing results confirmed that 28% of the mutant X chromosome remained active.ConclusionWe propose possible mechanisms of mutations associated with haploinsufficiency of LAMP2: (1) The inactivation X chromosome carrying the mutation was not significantly skewed. However, it decreased in the mRNA level and the expression ratio of the mutant transcripts; (2) The identified mutation is null, and the active mutant transcript fails to translate into the normal LAMP2 proteins. The presence of haploinsufficiency in LAMP2 and the X chromosome inactivation pattern were crucial factors contributing to the early onset of Danon disease in this female patient.
Prediction of experimental cardiac magnetostimulation thresholds using pig‐specific body models
Authors: Klein Valerie , Davids Mathias , Vendramini Livia , Ferris Natalie G. , Schad Lothar R. , Sosnovik David E. , Nguyen Christopher T. , Wald Lawrence L. , Guérin Bastien 
Published: June 2023

PurposeModern high‐amplitude gradient systems can be limited by the International Electrotechnical Commission 60601‐2‐33 cardiac stimulation (CS) limit, which was set in a conservative manner based on electrode experiments and E‐field simulations in uniform ellipsoidal body models. Here, we show that coupled electromagnetic‐electrophysiological modeling in detailed body and heart models can predict CS thresholds, suggesting that such modeling might lead to more detailed threshold estimates in humans. Specifically, we compare measured and predicted CS thresholds in eight pigs.MethodsWe created individualized porcine body models using MRI (Dixon for the whole body, CINE for the heart) that replicate the anatomy and posture of the animals used in our previous experimental CS study. We model the electric fields induced along cardiac Purkinje and ventricular muscle fibers and predict the electrophysiological response of these fibers, yielding CS threshold predictions in absolute units for each animal. Additionally, we assess the total modeling uncertainty through a variability analysis of the 25 main model parameters.ResultsPredicted and experimental CS thresholds agree within 19% on average (normalized RMS error), which is smaller than the 27% modeling uncertainty. No significant difference was found between the modeling predictions and experiments (p < 0.05, paired t‐test).ConclusionPredicted thresholds matched the experimental data within the modeling uncertainty, supporting the model validity. We believe that our modeling approach can be applied to study CS thresholds in humans for various gradient coils, body shapes/postures, and waveforms, which is difficult to do experimentally.