bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dedicated neuroimaging in children with primary headache: prevalence of lesions and comparison between migraine and non-migraine
Authors: Jeon Cha Woong , Lim Gye Yeon , Moon Ja Un 
Published: February 2023

Abstract
Purpose
This study evaluated the prevalence and types of intracranial lesions through dedicated imaging analysis of primary headaches in children and compared them between migraine and non-migraine patients.
Methods
This study included 190 children diagnosed with primary headache who underwent neuroimaging, including brain computed tomography (CT), CT angiography (CTA), and brain magnetic resonance imaging (MRI). Patients were divided into two groups based on data from electronic medical records. Clinical characteristics and imaging findings were evaluated and compared between the two groups.
Results
Migraine patients were old and had a longer period from symptom onset to diagnosis. CT was normal in 71 of 95 patients, whereas 7 of 29 patients who underwent CTA had vascular lesions; the migraine group (n = 6/20, 30%) had higher incidence of vascular lesions than the non-migraine group (n = 1/9, 11.1%); however, there was no statistically significant difference (p = 0.382). Furthermore, 57.5% (61/106) of children showed normal brain MRI. The most common brain MRI finding was dilated perivascular space (n = 18, 16.8%). Most perivascular spaces were located in the basal ganglia (n = 72, 75.8%) and were in linear patterns (n = 58, 63.0%). There was no statistically significant difference between the two groups.
Conclusion
Low prevalence of significant abnormalities was found in children with primary headaches. Dilated perivascular space was the most common finding in both groups on MRI. CTA showed more vascular lesions in the migraine group than in the non-migraine group. Therefore, further evaluations are needed to reveal the relationship between vascular lesions or dilated perivascular space and pediatric primary headaches.
Anchoring Ni3S2/Cr(OH)3 hybrid nanospheres on Ti3C2@NF dual substrates for efficient urea electrolysis by ion exchange
Authors: Liu Yifeng , Chen Qianqiao , Zhong Qin 
Published: February 2023

AbstractUrea-assisted hydrogen production is an energy-saving protocol, so it is particularly important to develop high-performance non-noble metal urea oxidation reaction (UOR) catalysts in simple ways. Herein, exploiting the surface electronegativity of Ti3C2, the electrode covering Ti3C2nanosheets and Ni3S2/Cr(OH)3nanospheres were synthesized on a nickel foam substrate by electrostatic adsorption and ion exchange for the first time, and both steps were completed within 2 hours at room temperature. The required overpotential are 130 mV and 169 mV at 10 mA·cm− 2for UOR and hydrogen evolution reaction(HER), respectively. It delivers current density of 10 mA·cm− 2at 1.52 V in a two-electrode system, and shows excellent stability. The superior performance of electrodes is mainly attributed to the synergy between the Ni3S2crystals and the Cr(OH)3amorphous structure, as well as the large adsorption surface and excellent electrical conductivity provided by the double substrate formed by the uniform coverage of Ti3C2on NF. The mild preparation process contributes excellent electrode stability to the protection of nickel foam structure. The ingenious structural design of Ni3S2/Cr(OH)3-Ti3C2@NF and the facile preparation method provide ideas for the development of low-cost and efficient electrodes with industrial application prospects.
Individualised 3d-printed Landmarks as Floating Duating Duoys for Poor Man's See-through Neuro-navigation in High Grade Glioma Surgery
Authors: KARAKAS Asli Beril , GOVSA Figen , BICEROGLU Huseyin , OZER Mehmet Asim , ERASLAN Cenk , BATI Ayse Hilal 
Published: February 2023

Abstract
Surgical treatment of gliomas requires an intervention that does not leave residual tumor tissue in the brain and preserves functional centers. Although orientation parameters are not present in the traditional-MRI navigation information calculated from MRI, poor mans are observed for the neuro-oncological surgery in most parts of the world. The aim of this study is to obtain instant personalized neuro-navigational information through a 3D-patient-specific model to perform functional resection of the brain. 20 patients diagnosed with gliomas were included in this study. Neuro-oncological navigation calculations of 10 patients were carried out with traditional-MRI support and the remaining 10 with the 3D-model. In this way, the actual size of the tumor, its distance from cortical structures, and perioperative surgical planning were made by the 20 neurosurgeons based on the patient-specific model. They were required to compare their perception level of the cases with traditional-MR and 3D-models in terms of identifying the invasion of the mass, making the proximity to functional centers and anatomical structures as part of perioperative planning. All neurosurgeons have given higher scores for 3D-model supported neuro-navigations. 80–90% of them preferred the model in preoperative planning as they are beneficial in anticipating determining and envisaging the entire process of the functional resection covering the location and extent of craniotomy, the extent of tumor resection on functional areas. For this, orientation parameters of the neuro-navigation information such as tumor size, margin size of surgical resection, presence of functional areas in the gyrus/sulcus where the tumor is located, proximity of the tumor, anatomical structures as (ventricles, arteries, veins, myelinated pathways, capsula interna, basal ganglia) should be included. It also target the perioperative advantages of 3D supported neuro-navigational information over the traditional method.
A Novel Cost-efficient Parallel Binary Adder without Crossover in QCA with Energy Dissipation Analysis
Authors: Chugh Hemanshi , Singh Sonal 
Published: February 2023

Abstract
Quantum dot cellular automata(QCA) is a computational paradigm which uses tiny semiconductor crystals as the basic units of information in computer circuits that can be used to process information similar to transistors. This work introduces a novel half-adder design in single-layer using 90◦ cells in a single clock zone. Moreover, a full adder circuit using 37 cells in one layer with 0.5 latency is proposed which further forms the basis of a nano-scale parallel binary adder(RCA) design in QCADesigner v2.0.3. The proposed 4-bit design uses 174 cells without the use of any cross-wiring with a latency of only 1 clock. The detailed energy dissipation study of the circuits is also investigated for different tunneling energies using the QCAPro tool. The comparison results of the adder circuits in respect of quantum cost, latency, cell count and total average energy dissipation show significant improvement as compared to the latest QCA-based adder designs.
Comparison of methods to prioritise plant diseases and their hosts for surveillance
Authors: Mitchell Ruth Joy 
Published: February 2023

Abstract
Non-native plant pests/pathogens are a mostly overlooked threat to biodiversity. Surveillance for plant diseases is key to early detection yet is rarely undertaken in semi-natural habitats. Currently there is no standard methodology available to help managers prioritise where surveillance should occur. This study compared five potential methods (M). Prioritisation of: pests/pathogens most likely to establish (M1); plant genera known to host the pests/pathogens most likely to establish (M2); habitats known to host the greatest number of pests/pathogens most likely to establish (M3); plants classed as foundation species (those that drive ecosystem functioning and support populations of dependent biodiversity) (M4); habitats with low plant species diversity and hence low resilience (M5). Twelve habitats and 22 heathland vegetation communities in the UK were used as a case-study. M1 gave 91 pests/pathogens to monitor and relied on having up-to-date lists of pests/pathogens relevant to plant species in semi-natural habitats. M2 gave 121 plant genera to monitor across all habitats and 14 within heathlands. M3 and M5 prioritised different habitats because M3 is based on existing lists of pests which are biased towards those of commercial importance. M4 gave 272 foundation species for surveillance across all habitats and 14 within heathlands. Surveillance of habitats and plants prioritised on potential ecological impact (M4-5) is recommended rather than known pests/pathogens (M2-3) as this avoids biases within existing lists of pests/pathogens, removes the need for the prioritisation to be regularly updated as new pests/pathogens are identified and takes account of impacts on associated biodiversity and ecosystem functions.
Application of a web-based multi-factor intelligent precision feeding system for fish
Authors: Liu Xiaojuan , Zhang Chuyi , Du Kaihua , Sha Zongyao , Luo Yi , Wang Chunfang F. 
Published: February 2023

Abstract
Precise feeding is an effective guarantee to improve the welfare of aquatic animals, increase culture efficiency and reduce environmental pollution, whereas internet of things (IoT) technology is an important means to promote the development of informatization and intelligence of aquaculture. In order to improve the precision, informatization and intelligence of fish feeding management, this paper proposed a multi-factor feeding system based on IoT technology, which integrates feeding management model, water quality monitoring system, fish feeding activity sensor, automatic feeding machine and software platform. Through the step-by-step regulation of general control + fine adjustment + precision adjustment to adjust the feeding amount, feeding speed and feeding duration of the automatic feeding machine, the system can not only achieve the precise and intelligent control of the feeding operation, but also realize the remote centralized management of cultured ponds or tanks. Through the comparison between the intelligent feeding system and the manual feeding system, the results showed that the use of the system not only improved the growth of fish, but also reduced the feed consumption and discharge of nitrogen and phosphorus wastes. The successful application of the feeding system confirms the effectiveness, convenience, and provides data and technical support for future modern and sustainable development of aquaculture.
Blinatumab versus chemotherapy in the treatment of relapsed and refractory acute lymphoblastic leukemia：A meta-analysis
Authors: li yongli , xing hongyun 
Published: February 2023

Abstract
Objective The objective of this study was to conduct an umbrella review of the efficacy and safety of Blinatumab in the treatment of relapsed/refractory acute lymphoblastic leukemia (R/R ALL) and provide evidence based reference for its clinical application.
Methods We searched PubMed, Cochrane,Embase,Web of Science,CNKI, WanFang, VIP database, and CBM from their inception to September 2022 to identify randomized controlled trials (RCTs) where the effects of Blinatumab (experimental groups ) versus traditional chemotherapy (control group) for R/R ALL were collected. The risk of bias was evaluated according to the Revised Cochrane risk-of-bias tool for randomized trials (RoB2).The RevMan 5.4 software was used to perform meta-analysis.
Results A total of 1622 R/R ALL patients were included in this study,1094 patients received Blinatumab and 528 patients received chemotherapy.Compared with traditional chemotherapy, Blinatumab had longer mOS (HR=0.68; 95% CI, 0.57-0.81) (P<0.0001), better EFS (HR=0.57; 95% CI, 0.42-0.76) (P=0.0002),better CR. and the negative rate of MRD was higher (OR=4.49; 95% CI, 3.09-6.51) (P<0.0001);Blinatumomab had lower incidence of anemia (OR=0.10; 95% CI, 0.04-0.25) (P<0.0001), mucositis (OR=0.07; 95% CI, 0.03-0.20) (P<0.0001), infection (OR=0.06; 95% CI, 0.04-0.11) (P<0.0001), neutropenic fever (OR=0.03; 95% CI, 0.01-0.06)(P<0.0001),neutropenia (OR=0.18; 95% CI, 0.12-0.27) (P<0.0001), thrombocytopenia(OR=0.06; 95% CI, 0.01-0.60) (P=0.02),and sepsis (OR=0.13; 95% CI, 0.07-0.26) (P<0.0001),while the incidence of Cytokine release syndromehigher and Adverse events of nervous system were higer.
Conclusion Overall, the safety and effectiveness of blinatumomab for R/R ALL is better than that of traditional chemotherapy.
FTO upregulation induced by MYC suppresses tumour progression in Epstein‒Barr virus-associated gastric cancer
Authors: Xu Yun-Yun , Li Ting , Shen Ao , Bao Xiao-Qiong , Lin Jin-Fei , Guo Li-Zhen , Meng Qi , Ruan Dan-Yun , Zhang Qi-Hua , Zuo Zhi-Xiang , Zeng Zhao-lei 
Published: February 2023

Abstract
Background: Epstein‒Barr virus-associated gastric cancer (EBVaGC) is regarded as a distinct molecular subtype of GC, accounting for approximately 9% of all GC cases, and it often exhibits unique molecular features and clinicopathological characteristics. Clinically, EBVaGC patients are found to have a significantly lower frequency of lymph node metastasis and better prognosis than uninfected individuals. RNA N6-methyladenosine (m6A) modification has an indispensable role in modulating tumour progression in various cancer types. However, its impact on EBVaGC remains unclear.
Methods: Methylated RNA immunoprecipitation sequence (MeRIP-seq) and m6A dot blot were conducted to compare the m6A modification levels between EBVaGC and EBV-negative GC (EBVnGC) cells. Western blot, real-time quantitative PCR (RT-qPCR) and immunohistochemistry were applied to explore the underlying mechanism of the reduced m6A modification in EBVaGC. The biological function of FTO was determined in vivo and in vitro. The target genes of FTO were screened by MeRIP-seq, RT-qPCR and Western blot. The m6A binding proteins of target genes were verified by RNA pulldown and RNA immunoprecipitation assay. Chromatin immunoprecipitation and Luciferase report assay were performed to investigate the mechanism how EBV upregulated FTO expression.
Results: Here, we found that m6A demethylase fat mass and obesity associated protein (FTO) was notably increased in EBVaGC, leading to a reduction in m6A modification, and higher FTO expression was associated with better clinical outcomes. Our findings revealed that FTO depressed EBVaGC cell metastasis and aggressiveness both in vitro and in vivo by controlling the expression of its downstream target gene AP-1 transcription factor subunit (FOS). Methylated FOS mRNA was specifically recognized by the m6A “reader” insulin-like growth factor 2 mRNA binding protein 1/2 (IGF2BP1/2), which enhanced its transcript stability. Moreover, we demonstrated that MYC activated by EBV in EBVaGC elevated FTO expression by binding to a specific region of the FTO promoter.
Conclusions: Mechanistically, our work uncovered a crucial suppressive role of FTO in EBVaGC metastasis and invasiveness via an m6A-FOS-IGF2BP1/2-dependent manner, suggesting a promising biomarker panel for GC metastatic prediction and therapy.
Stressed target cancer cells drive nongenetic reprogramming of CAR T cells and tumor microenvironment, overcoming multiple obstacles of CAR T therapy for solid tumors
Authors: Wang Yufeng , Drum David L. , Sun Ruochuan , Zhang Yida , Yu Ling , Jia Lin , Isakoff Steven J , Kehlmann Allison M , Dal Ali Emre , Dotti Gianpietro , Zheng Hui , Ferrone Cristina R , Taghian Alphonse G , DeLeo Albert B , Zhang Hanyu , Jounaidi Youssef , Fan Song , Huang Peigen , Wang Cheng , Yang Jibing , Boland Genevieve M , Sadreyev Ruslan I , Wong LaiPing , Ferrone Soldano , Wang Xinhui 
Published: February 2023

Abstract
The poor efficacy of chimeric antigen receptor T-cell therapy (CAR T) for solid tumor is due to insufficient CAR T cell tumor infiltration, in vivo expansion, persistence, and effector function, as well as exhaustion, intrinsic target antigen heterogeneity or antigen loss of target cancer cells, and immunosuppressive tumor microenvironment (TME). Here we describe a broadly applicable nongenetic approach that simultaneously addresses the multiple challenges of CAR T as a therapy for solid tumors. The approach massively reprograms CAR T cells by exposing them to stressed target cancer cells which have been exposed to the cell stress inducer disulfiram (DSF) and copper (Cu)(DSF/Cu) plus ionizing irradiation (IR). The reprogrammed CAR T cells acquired early memory-like characteristics, potent cytotoxicity, enhanced in vivo expansion, persistence, and decreased exhaustion. Tumors stressed by DSF/Cu and IR also reprogrammed and reversed immunosuppressive TME in humanized mice. The reprogrammed CAR T cells, derived from peripheral blood mononuclear cells (PBMC) of healthy or metastatic breast cancer patients, induced robust, sustained memory and curative anti-solid tumor responses in multiple xenograft mouse models, establishing proof of concept for empowering CAR T by stressing tumor as a novel therapy for solid tumor.
Astragaloside IV improves cognitive impairment caused by CCH via improve ROS and NLRP3 pathway by up-regulating the PGC1α/Nrf2 pathway
Authors: Meng Nan , Li Meixi , Xu Jing , Guan Tianyuan , Jin Man , Teng Zhenjie , Zhao Lei , Fan Mingyue , Hao Hongyu , Lv Peiyuan 
Published: February 2023

Abstract
This study was conducted to elucidate the possible molecular mechanisms of neuroprotective effect of Astragaloside IV (As-IV) on animal models of Vascular Dementia . Oxidative damage and neuroinflammation play a key role in chronic cerebral hypoperfusion (CCH) and lead to a decline in cognitive function in animals. In the present study, Sprague Dawley (SD) rats were randomly divided into sham group, model group, As-IV20 group, and As-IV50 group, with 10 rats in each group. The Morris water maze (MWM) was used to evaluate the cognitive function of rats. The nitrogen-blue tetrazolium photoreduction method was employed to detect the levels of superoxide dismutase (SOD) in the hippocampal tissue homogenate. The bituric acid method was utilised to detect the malondialdehyde (MDA) levels. Western blotting was performed to evaluate the levels of stimulator of interferon genes (Sting), NOD-like receptor protein 3 (NLRP3)，Caspase-1 and Peroxisome proliferator-activated receptor gamma co-activator-1α (PGC-1α)，and transcription factors such as nuclear factor erythroid-derived factor-2 (Nrf2) in hippocampus tissues. The results indicate that As-IV supplementation significantly regulates the above changes. In addition, As-IV treatment effectively up-regulates the PGC-1α and Nrf2. Overall, our findings suggest that As-IV can effectively improve the cognitive impairment caused by CCH and oxidative damage. Furthermore, mitochondrial damage and inflammation against CCH may be attributed to the activation of the PGC-1α/Nrf2 signalling pathway to reduce ROS. This activation can reduce neuroinflammation caused by Sting/NLRP3/caspase1 pathway.
Accumulation of prion triggers the enhanced glycolysis via activation of AMKP pathway in prion infected rodent and cell models
Authors: Fan Qin , Xiao Kang , Ruhan A , Gao Li-Ping , Wu Yue-Zhang , Chen Dong-Dong , Hu Chao , Jia Xiao-Xi , Liu Chu-Mou , Liu Xin , Cao Chen , Shi Qi , Dong Xiao-ping 
Published: February 2023

Abstract
Mitochondrial dysfunction is one of hallmarks in pathophysiology of prion disease and other neurodegenerative diseases. Various metabolic dysfunctions are identified and considered to contribute to the progression of some types of neurodegenerative diseases. In this study, we evaluated the status of glycolysis pathway in prion infected rodent and cell models. The levels of the key enzymes, hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) were significantly increased, accompanying with markedly downregulated mitochondrial complexes. Double stained IFAs revealed that the increased HK2 and PFK distributed widely in GFAP-, Iba1- and NeuN-positive cells. We also identified increased levels of AMP-activated protein kinase (AMPK) and the downstream signaling. Changes of AMPK activity in prion infected cells by the AMPK-specific inhibitor or activator induced the corresponding alterations not only in the downstream signaling, but also the expressions of three key kinases in glycolysis pathway and the mitochondrial complexes. Transient removal or complete clearance of prion propagation in the prion infected cells partially but significantly reversed the increases of the key enzymes in glycolysis, the upregulation of AMPK signaling pathway, and the decreases of the mitochondrial complexes. Measurements of the cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) showed lower OCR and higher ECAR in prion infected cell line, which were sufficiently reversed by clearance of prion propagation. Those data indicate a metabolic reprogramming from oxidative phosphorylation to glycolysis in the brains during the progression of prion disease. Accumulation of PrPSc is critical for the switch to glycolysis, largely via activating AMPK pathway.
Behavioral and Emotional Difficulties and HIV Treatment Outcomes among HIV-Infected Children in Rural Southwest China
Authors: Zhou Yesheng , Tang Kailing , Lu Hongyan , Chen Hongli , Xie Haomin , Li Zeyu , Huang Jinghua , Fang Ningye , Chen Siya , Wang Hong , He Qin , Chen Huanhuan , Liu Xiu , Lan Guanghua , Zhu Qiuying , Chen Yi , Zhang Xiangjun , Ruan Yuhua , Liang Shujia 
Published: February 2023

Abstract
Background
Previous studies have not clearly demonstrated the impact of behavioral and emotional problems (BEDs) on treatment outcomes among HIV-infected children on antiretroviral therapy (ART). This study aimed to describe the prevalence of BEDs among this population and identify the factors associated with HIV treatment outcomes.
Methods
This cross-sectional study was conducted in Guangxi, China, between July and August 2021. HIV-infected children answered questionnaires about BEDs, physical health, social support, and whether they have missed doses in the past month. BEDs were assessed using the Chinese version of the self-reported Strengths and Difficulties Questionnaire (SDQ-C). Univariate and multivariate logistic regression models were used to identify factors that were associated with missed doses in the past month and virological failure.
Results
The study sample was 325 HIV-infected children. HIV-infected children had a higher proportion of abnormal scores on SDQ-C total difficulties compared to their peers in the general population (16.9 vs 10.0%; P = 0.002). An abnormal SDQ-C total difficulties score (AOR = 2.06, 95%CI: 1.10–3.88) and infrequency of receiving assistance and support from parents over the past 3 months (AOR = 1.85, 95%CI: 1.12–3.06) were significantly associated with missed doses in the past month. Between the ages of 14–17 years (AOR = 2.66, 95% CI: 1.37–5.16), female (AOR = 2.21, 95% CI: 1.20–4.08), and suboptimal adherence (AOR = 2.45, 95% CI: 1.32–4.57) were significantly associated with virological failure.
Conclusions
Children’s mental health plays a role in HIV treatment outcomes. Psychological interventions should be promoted in pediatric HIV care clinics to improve children’s mental health status and HIV treatment outcomes.