bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of ferroptosis-related diagnostic biomarkers in ischemic cardiomyopathy Abstract
Authors: Ma Guizhou , Zhou Linjie , Xu Ronghe , Wang Ying , Cai Zhixiong 
Published: March 2023

Abstract
Background:
Ischemic cardiomyopathy (ICM), the frequently observed secondary cardiomyopathy, is a major factor leading to global mortality. Ferroptosis is a type of iron-dependent programmed cell death different from apoptosis or additional programmed cell death types, identified as the major cell death type of cardiomyocytes. Consequently, the present study focused on the identification of ferroptosis-related genes (FRGs) and determining their value in diagnosing ICM, thus, assisting in detecting and treating ICM.
Methods:
This study collected gene expression profiles for ventricular myocardium specimens in ICM cases and normal participants from the gene expression omnibus database. First, ferroptosis-related differentially expressed genes (FRDEGs) were detected in ICM. Then, a protein-protein interaction (PPI) network related to FRDEGs was constructed through PPI network analysis, followed by the identification of the key network and hub genes whose expression was analyzed and assessed using receiver operating characteristic (ROC) curve analysis and verified in the external validation set. Finally, hub genes were subject to single-gene GSEA pathway enrichment, targeted drug prediction, construction of miRNA as well as transcription factor regulatory network.
Results:
A total of 1396 DEGs were detected, among which 711 showed up-regulation, whereas 685 displayed down-regulation in patients with ICM compared with healthy controls. After overlapping with FRGs, 39 FRDEGs were obtained. Based on the gene Ontology function together with the Kyoto encyclopedia of genes and genomesanalysis, the aforementioned genes were mostly associated with starvation, cellular extracellular stimulus, and cellular starvation responses, peroxisome proliferators-activated receptors signaling pathway, human cytomegalovirus infection, and hepatitis C. Eight hub genes were obtained from the PPI network related to FRDEGs. Hub gene expression was down-regulated in the ICM. ROC curves showed that the area under the curve of activating transcription factor 4 (ATF4), signal transducers and activators of transcription 3 (STAT3), CCAAT enhancer binding protein gamma, X-box binding protein 1 (XBP1), and eukaryotic translation initiation factor 2 subunit alphawas greater than 0.7. After verification in the external dataset, XBP1, ATF4, and STAT3 were screened as the diagnostic genes for ICM.
Conclusion:
The XBP1, STAT3, and ATF4 were identified as potential diagnostic genes of ICM. Thus, these genes may provide novel markers or targets to diagnose and treat ICM.
Multiphase CT radiomics nomogram for preoperatively predicting the WHO/ISUP nuclear grade of small (&lt; 4 cm) clear cell renal cell carcinoma
Authors: Gao Yankun , Wang Xia , Zhao Xiaoying , Zhu Chao , Li Cuiping , Li Jianying , Wu Xingwang 
Published: March 2023

Abstract
Background
To develop and validate a CT-based radiomics nomogram for preoperatively predicting WHO/ISUP nuclear grade in small (< 4 cm) clear cell renal cell carcinoma (ccRCC).
Methods
A total of 113 patients with histologically confirmed ccRCC were randomly assigned to the training set (n=67) and the testing set (n=46). The baseline and CT imaging data of the patients were evaluated statistically to develop a clinical model. A radiomics model was created, and the radiomics score (Rad-score) was calculated by extracting radiomics features from the CT images. Then, a clinical radiomics nomogram was developed using multivariate logistic regression analysis by combining the Rad-score and critical clinical characteristics. The receiver operating characteristic (ROC) curve was used to evaluate the discrimination of small ccRCC in both the training and testing sets.
Results
The radiomics model was constructed using six features obtained from the CT images. The shape and relative enhancement value of the nephrographic phase (REV of the NP) were found to be independent risk factors in the clinical model. The area under the curve (AUC) values for the training and testing sets for the clinical radiomics nomogram were 0.940 and 0.902, respectively. Decision curve analysis (DCA) revealed that the radiomics nomogram model was a better predictor, with the highest degree of coincidence.
Conclusion
The CT-based radiomics nomogram has the potential to be a noninvasive and preoperative method for predicting the WHO/ISUP grade of small ccRCC.
Community perceptions of a biopsychosocial model of integrated care in the health center: the case of 4 health districts in South Kivu, Democratic Republic of Congo.
Authors: Kasongo Bertin , Molima Christian , Makali Samuel , Chiribagula Christian , Mparanyi Gérard , Karemere Hermès , Bisimwa Ghislain , Mukalay Abdon 
Published: March 2023

Abstract
Background: The concept of biopsychosocial care is one of the approaches recommended in the health system by the WHO. Although efforts are being made on the provider side to implement it, community involvement is needed to enable its integration into the health sector. For this to happen, the community must first have an understanding of the approach, as a stakeholder and direct beneficiary. The objective of this study is to understand the community's views on the concept of integrated health care according to the biopsychosocial approach (BPS) at the Health Center of a Health District.
Methods: We conducted a qualitative study based on individual semi-directive interviews with members of the Health Development Committees of six Health Areas belonging to four Health Districts as well as with some patients met in health facilities. A total of 15 interviews were conducted. The adapted NoMAD tool, derived from the Theory of the Normalization Process of Complex Interventions, allowed us to collect data from November 2017 to February 2018, and then from November 2018 to February 2019. After data extraction and synthesis, we conducted a thematic analysis using the NoMAD tool to build a thematic framework.
Results: Initially, community perceptions were diverse in relation to the BPS approach of integrated care in the Health Centre; but later, the concept became clearer with the implementation of the approach, even for the providers with the change in their way of working (interprofessional collaboration, sharing of responsibilities...). Certain practices were encouraged to help the approach, notably the development of financial autonomy and mutual support. According to the community, the BPS model has further strengthened the performance of health workers and should be expanded and sustained. Stakeholders throughout the country were called upon to support the BPS concept in its implementation.
Conclusions: The results of our study should encourage the involvement of community participation in any process of integrating the biopsychosocial model of person-centred health care, even at higher levels of care. However, the barriers and enablers to the BPS mechanism identified in our study should be taken into consideration.
Modulation of the rumen microbiome of dairy cows by inclusion of a live yeast supplement in a total mixed ration
Authors: Johnson Catherine A. , Snelling Timothy J. , Warren Helen , Taylor-Pickard Jules , Huntington James A. , Sinclair Liam A. 
Published: March 2023

Abstract
High yielding dairy cattle are typically fed a total mixed ration (TMR) to meet their energy requirements. However, inadequate mixing can lead to feed selection and dietary imbalance, negatively affecting rumen metabolism and the microbiome. To investigate the effect of TMR mixing, four dairy cows were fed a partial mixed ration with dietary concentrates (4 kg/cow/day) added separately in even and uneven patterns of allocation (CA) with a Saccharomyces cerevisiae yeast supplement (YS) (1 g/cow/day). Rumen digesta samples were taken to measure microbial metabolism and microbiome using16S rRNA amplicon sequencing. No effect of CA on rumen metabolism was detected. YS inclusion tended to increase rumen pH (P= 0.088) reduce total VFA concentration (P= 0.033) and propionate concentration (P= 0.016). Clustering of the rumen microbiome was observed with YS supplementation, driven by a decrease in abundance of Gammaproteobacteria and Prevotellaceae OTUs respectively and an increase of a Christensenellaceae OTU. Pattern of concentrate allocation had no detectable effect on alpha diversity or Bray Curtis dissimilarity. However, reduction in relative abundance of a Prevotellaceae OTU biomarker was associated with uneven pattern of concentrate allocation. Pattern of concentrate allocation and YS supplementation did not adversely affect milk yield or composition.
Observational Evidence of the Prevalence of Bipolar Galactic Outflows out to 10 kpc at z ≈ 1 for Massive Galaxies
Authors: Guo Yucheng , Bacon Roland , Bouché Nicolas , Wisotzki Lutz , Schaye Joop , Blaizot Jérémy , Verhamme Anne , Cantalupo Sebastiano , Boogaard Leindert , Brinchmann Jarle , Cherrey Maxime , Kusakabe Haruka , Langan Ivanna , Leclercq Floriane , Matthee Jorryt , Michel-Dansac Léo , Schroetter Ilane , Wendt Martin 
Published: March 2023

Abstract
Galactic outflows are believed to play a critical role in the evolution of galaxies by regulating their mass build-up and star formation [1]. These outflows tend to form bipolar shapes above and below the galactic planes and extend well into the circumgalactic medium (CGM), up to tens of kpc perpendicular to the galaxy. They have been directly observed in the local Universe, e.g., around the Milky Way and M82 [2, 3]. At higher redshifts, cosmological simulations of galaxy formation predict an increase in the frequency and efficiency of galactic outflows due to the increasing star formation activity [4]. Outflows are responsible for removing potential fuel for star formation from the galaxy, while at the same enriching the CGM and the intergalactic medium. These feedback processes, although incorporated as key elements of cosmological simulations, are still poorly constrained on CGM scales. Here we present an ultra-deep MUSE image of the mean Mg II emission surrounding a sample of galaxies at z ≈ 1 that strongly suggests the presence of outflowing gas on physical scales of more than 10 kpc. We find a strong dependence of the detected signal on the inclination of the central galaxy, with edge-on galaxies clearly showing enhanced Mg II emission along the minor axis, while face-on galaxies display much weaker and more isotropic emission. We interpret these findings as supporting the idea that outflows typically have a bipolar cone geometry perpendicular to the galactic disk. We demonstrate that the signal is not dominated by a few outliers. After dividing the galaxy sample in subsamples by mass, the bipolar emission is only detected in galaxies with stellar mass M* >∼ 109.5 M⊙.
Production profile and comparison analysis of main toxin components of Fusarium oxysporum f. sp. sesami isolates with different pathogenicity levels
Authors: Li Hailing , Duan Yinghui , Xu Guizhen , Chang Shuxian , Ju Ming , Wu Yin , Qu Wenen , Cao Hengchun , Zhang Haiyang , Miao Hongmei 
Published: March 2023

Abstract
Fusarium wilt is a critical fungus disease for sesame, which is induced by Fusarium oxysporum f. sp. sesami (FOS). In order to determine the toxin production profiles of the FOS isolates with different pathogenicity levels under various culture conditions, we evaluated the content variation of fusaric acid (FA) and 9, 10-dehydrofusaric acid (9, 10-DFA) produced by the 4 representative FOS isolates. Results indicated that the maximum amount of FA reached to 2848.66 µg/ml in Czapek medium, while 9, 10-DFA was mainly produced in Richard and Low-carbon Richard medium. The concentration of 9, 10-DFA on Richard culture medium varied from 0 µg/ml to 716.89 µg/ml. Of the 5 kinds of culture media, Czapek culture medium was the most conductive to produce FA. FA production was significantly affected by culture medium, culture time, and their interaction (***, P < 0.001). The results suggest that there is no correlation between toxin production and pathogenicity level of FOS isolates. The findings give key information for the mechanism analysis of FOS- sesame interaction and pathogen control.
NADPH oxidase subunit p22phox gene C242T polymorphism and vascular complications in diabetes: a color Doppler ultrasound study
Authors: Xue Lu , Chongjun Xu , Baoding Shan , Zhang Shuhe , Wang Siqi , Guangming Jin 
Published: March 2023

Abstract
Background
To investigate the distribution of NADPH oxidase subunit p22phox gene C242T polymorphisms in T2DM patients and analyze the association of NADPH oxidase subunit p22phox gene C242T polymorphisms with vascular complications in diabetic patients.
Methods
We analyzed 273 patients with T2DM; these were divided into four groups: a normal IMT group, a thickened IMT group, a non-carotid atherosclerotic plaque group and a carotid atherosclerotic plaque group. Of the 273 patients, there were 186 cases of diabetic nephropathy (DN) and 212 cases of diabetic retinopathy (DR). The patients in each group were genotyped for NADPH oxidase subunit p22phox by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). After that, the DN and DR Patients were divided into 2 groups: CC genotype and the CT + TT genotype, respectively. Then, maximal systolic velocity (Vmax), minimal diastolic velocity (Vmin), mean velocity (Vmean), time velocity integral (TVI), resistance index (RI) and Pulse index (PI) were measured by Doppler ultrasound in the left renal artery (LRA) of DN patients and the central retinal artery (CRA) of DR patients.
Results
There was a significant difference between the normal IMT group and the thickened IMT group (P < 0.05) with regards to NADPH oxidase subunit p22phox gene C242T polymorphism. There was also a significant difference between the non-carotid atherosclerotic plaque group and thecarotid atherosclerotic plaque group (P<0.05) with regards to NADPH oxidase subunit p22phox gene C242T polymorphism. Further analysis showed that CRA Vmax, Vmin, VTI decreased, PI and RI increased in CC genotype DR patients, which were significantly different from those in CT+TT genotype DR patients. CC genotype DN patients showed the same results as CT+TT genotype DN patients (P<0.05, P<0.001).
Conclusion
The polymorphism of NADPH oxidase subunit p22phox gene is associated with carotid atherosclerosis in patients with T2DM, and can lead to the occurrence of DR and DN by affecting the hemodynamics of CRA and RA. Monitoring vascular conditions in patients with T2DM by CDUS can provide effective early warning information for patients to prevent vascular complications.
Remodeling of venous drainage after the treatment of dural sinus malformation with arteriovenous shunts in a neonate: A case report and literature review
Authors: Hanai Sho , Muroi Ai , Hitaka Daisuke , Murakami Takashi , Sato Masayuki , Matsumaru Yuji , Ishikawa Eiichi 
Published: March 2023

Abstract
Dural sinus malformations (DSMs) are rare congenital vascular diseases characterized by a giant venous pouch with or without arteriovenous shunts. We present a neonatal case of DSM that was diagnosed prenatally and treated via endovascular intervention in the early postnatal period. The patient presented with a large DSM involving the torcular Herophili on prenatal magnetic resonace imaging (MRI). Enlargement of the head circumference and respiratory failure rapidly progressed after birth. On the five day after birth, the neonate underwent endovascular occlusion via the umbilical artery. The arteriovenous shunt was occluded, and the reflux from the enlarged venous pouch to the dural sinus was decreased. No additional procedure other than ventriculoperitoneal shunting was required. The neonate’s development slowly caught up to normal parameters. Follow-up MRI demonstrated the successful development of the venous drainage system. DSMs are characterized by an abnormally dilated dural sinus, which can block the venous return and ultimately increase intracranial pressure and cerebral ischemia. Long-term follow-up indicates that an abnormally developed dural sinus can be reconstructed by appropriate and timely treatment.
USP51 promotes non-small-cell lung carcinoma cell stemness by deubiquitinating Twist1
Authors: Chen Jin , Wu Zhongqiu , Deng Wenyi , Tang Minying , Wu Lvying , Lin Na , Chen Liuyan , Fu Yunfeng , Zhao Min , Chen Changguo , Li Wenting 
Published: March 2023

Abstract
Background
USP51 is a deubiquitinase (DUB), which is involved in diverse cellular processes. Accumulating evidence has demonstrated that USP51 contributes to cancer development. However, its impact on non-small-cell lung carcinoma (NSCLC) cell malignancy is largely unknown.
Methods
In this study, we performed bioinformatics analysis on a TCGA (The Cancer Genome Atlas) dataset to determine the association between USP51 and cell stemness markers in NSCLC patients. RT-qPCR, western blotting, and flow cytometry were performed to examine the effects of USP51 depletion on stemness marker expression. Colony formation and tumor sphere formation assays were used to assess the stemness of the NSCLC cells. Time-course assays directed by cycloheximide and polyubiquitination experiments were carried out to analyze the effects of USP51 on the TWIST1 protein. TWIST1 was overexpressed in USP51 knockdown NSCLC cells to determine whether TWIST1 is required. The effect of USP51 on the in vivo growth of NSCLC cells was tested through subcutaneous injections in mice.
Results
We found that USP51 expression was positively correlated with several stemness markers in NSCLC patients. USP51 depletion attenuated the stemness marker RNA, protein, and cell surface expression and stemness of NSCLC cells. Ectopic USP51 expression potentiated the TWIST1 protein stability by mitigating its polyubiquitination. In addition, the TWIST1 re-expression in NSCLC cells rescued the stemness-inhibitory effect of USP51 knockdown. Furthermore, in vivo results confirmed the suppressive effect of USP51 depletion on NSCLC cell growth.
Conclusions
Our results showed that USP51 knockdown alleviated NSCLC cell stemness by deubiquitinating TWIST1.
Electrochemically induced crystalline-to-amorphization transformation in sodium samarium silicate solid electrolyte for long-lasting all-solid-state sodium metal batteries
Authors: Sun Ge , Lou Chenjie , Wei Zhixuan , Yao Shiyu , Lu Ziheng , Chen Gang , Shen Ze , Tang Mingxue , Du Fei 
Published: March 2023

Abstract
Exploiting solid electrolyte (SE) materials with high ionic conductivity, good interfacial compatibility, and ultraconformal contact with electrode are essential for solid-state sodium metal batteries (SSBs). Here we report a crystalline Na5SmSi4O12 SE which features high room-temperature ionic conductivity of 2.90×10-3 S cm-1 and a low activation energy of 0.15 eV. All-solid-state symmetric cell with Na5SmSi4O12 delivers excellent cycling life over 800 h at 0.15 mA h cm-2 and high critical current density of 1.4 mA cm-2. Such excellent electrochemical performance is attributed to an electrochemically induced in-situ crystalline-to-amorphous (CTA) transformation propagating from the interface to the bulk during repeated deposition and stripping of sodium, which lead to faster ionic transport and superior interfacial properties. Impressively, the Na3V2(PO4)3||Na5SmSi4O12||Na SSBs achieves a remarkable cycling performance over 4000 cycles (6 months) with no capacity loss. These results not only identify Na5SmSi4O12 as a promising SE, but also emphasize the potential of the CTA transition as a promising mechanism towards long-lasting SSBs.
Effectiveness of Gender Budgeting in Sub-Saharan Africa
Authors: AMBASSA Laurent-Fabrice , ESSIMI Jean Aristide BILOA , GANKOU Jean Marie 
Published: March 2023

Abstract
The aim of this study is to verify the impact of gender budgeting on gender inequalities and on public spending in Sub-Saharan Africa. The analysis covers a sample of thirty (30) countries in Sub-Saharan Africa covering the period 2000–2015. The results obtained using a panel data analysis by the generalized method of moments (GMM) in difference and in system show that gender budgeting significantly reduces gender inequalities in Sub-Saharan Africa.
JEL CLASSIFICATION: H00; I00; J16
Improved artificial intelligence discrimination of minor histological populations by supplementing with color-adjusted images
Authors: Hatta Satomi , Ichiuji Yoshihito , Mabu Shingo , Kugler Mauricio , Hontani Hidekata , Okoshi Tadakazu , Fuse Haruki , Kawada Takako , Kido Shoji , Imamura Yoshiaki , Naiki Hironobu , Inai Kunihiro 
Published: March 2023

Abstract
Despite the dedicated research of artificial intelligence (AI) for pathological images, the construction of AI applicable to histopathological tissue subtypes, is limited by insufficient dataset collection owing to disease infrequency. Here, we present a solution involving the addition of supplemental tissue array (TA) images that are adjusted to the tonality of the main data using a cycle-consistent generative adversarial network (CycleGAN) to the training data for rare tissue types. F1 scores of rare tissue types that constitute < 1.2% of the training data were significantly increased by improving recall values after adding color-adjusted TA images constituting < 0.65% of total training patches. The detector also enabled the equivalent discrimination of clinical images from two distinct hospitals and the capability was more increased following color-correction of test data before AI identification (F1 score from 45.2 ± 27.1 to 77.1 ± 10.3, p<0.01). These methods also classified intraoperative frozen sections, while excessive supplementation paradoxically decreased F1 scores. These results identify strategies for constructing AI with an imbalance among the training data, which is important for constructing AI for practical histopathological classification.
A framework for locating prescribed medication at pharmacies
Authors: Wondmagegn Matiwos Tekalign , Nabassa Evarist , Melesse Endashaw Amsalu , Kassaw Ewunate Assaye 
Published: March 2023

Abstract
The availability of medication at pharmacies is one of the major problems in the health sector of developing countries. Patients are frequently forced to arbitrarily switch pharmacies in search of the prescribed prescription. Lack of hospital and pharmacy drug information communication and inventory systems, as well as a lack of knowledge about the locations of pharmacies that carry the necessary medications, are some of the issues. The goal of this project is to provide a framework that will make it easier for anyone looking for a local pharmacy to find and locate one. The framework was created using the primary obstacles to obtaining necessary prescribed medications identified in the literature. The framework uses the client's and pharmacies' latitude and longitude coordinates to find the closest pharmacies that have the required prescribed medication in stock. To achieve this, the identified restrictions are optimized (distance, drug cost, travel time, travel cost, opening and closing hours of nearby pharmacies). The framework may reduce patient expenditures and prevent delays in the delivery of medications. It will also pave the way for future pharmacy and e-Health information systems.