bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinically Translatable Transcrocetin Delivery Platform for Correction of Tumor Hypoxia and Enhancement of Radiation Therapy Effects
Authors: Diringer Marie‐Charlotte , Coliat Pierre , Mathieu Clélia , Laurent Nina , Mura Carole , Banerjee Mainak , Zhu Chen , Grabowska Anna , Ritchie Alison , Clarke Philip , Bernard Alexandre , Vit Claire , Burckel Hélène , Noel Georges , Harvey Peter , Pivot Xavier , Detappe Alexandre 
Published: January 2023

AbstractImproving the tumor reoxygenation to sensitize the tumor to radiation therapy is a cornerstone in radiation oncology. Here, the pre‐clinical development of a clinically transferable liposomal formulation encapsulating trans sodium crocetinate (NP TSC) is reported to improve oxygen diffusion through the tumor environment. Early pharmacokinetic analysis of the clinical trial of this molecule performed on 37 patients orient to define the optimal fixed dosage to use in a triple‐negative breast cancer model to validate the therapeutic combination of radiation therapy and NP TSC. Notably, it is reported that this formulation is non‐toxic in both humans and mice at the defined fixed concentration, provides a normalization of the tumor vasculature within 72 h window after systemic injection, leads to a transient increase (50% improvement) in the tumor oxygenation, and significantly improves the efficacy of both mono‐fractionated and fractionated radiation therapy treatment. Together, these findings support the introduction of a first‐in‐class therapeutic construct capable of tumor‐specific reoxygenation without associated toxicities.
Critical Influence of Organic A′‐Site Ligand Structure on 2D Perovskite Crystallization
Authors: Qin Zhaotong , Xue Haibo , Qin Minchao , Li Yuhao , Wu Xiao , Wu Wei‐Ru , Su Chun‐Jen , Brocks Geert , Tao Shuxia , Lu Xinhui 
Published: January 2023

AbstractOrganic A′‐site ligand structure plays a crucial role in the crystal growth of 2D perovskites, but the underlying mechanism has not been adequately understood. This problem is tackled by studying the influence of two isomeric A′‐site ligands, linear‐shaped n‐butylammonium (n‐BA+) and branched iso‐butylammonium (iso‐BA+), on 2D perovskites from precursor to device, with a combination of in situ grazing‐incidence wide‐angle X‐ray scattering and density functional theory. It is found that branched iso‐BA+, due to the lower aggregation enthalpies, tends to form large‐size clusters in the precursor solution, which can act as pre‐nucleation sites to expedite the crystallization of vertically oriented 2D perovskites. Furthermore, iso‐BA+ is less likely to be incorporated into the MAPbI3 lattice than n‐BA+, suppressing the formation of unwanted multi‐oriented perovskites. These findings well explain the better device performance of 2D perovskite solar cells based on iso‐BA+ and elucidate the fundamental mechanism of ligand structural impact on 2D perovskite crystallization.
Quantifying detection probability of American woodcock (Scolopax minor) on transects sampled with thermal cameras
Authors: Gray Luke F. , McNeil Darin J. , Larkin Jeffery T. , Parker Halie A. , Shaffer Dakotah , Larkin Jeffery L. 
Published: January 2023

AbstractDeveloping effective monitoring techniques for sensitive wildlife populations is essential for improving conservation outcomes. The American woodcock (Scolopax minor; hereafter woodcock) is an upland migratory game bird traditionally surveyed by documenting displaying males in spring. Surveys of displaying males are limiting in a variety of important ways such as brief detection window and male‐centric observations. Thermal technology may overcome limitations of traditional monitoring techniques by increasing detections of non‐singing woodcock, however, the efficacy of thermal imaging for detecting woodcock remains unknown. To quantify woodcock detection probability using thermal imaging, we deployed and searched for heat‐emitting woodcock mounts along transects within early‐successional habitats in central Pennsylvania during 2020. We deployed 110 woodcock mounts and successfully detected 63 (57.2%). Detection rate declined as a function of increasing vegetation density and distance from transect. Although detection probability of woodcock was imperfect, thermal cameras may provide a solution for researchers aiming to assess presence or density of woodcock when coupled with analytical methods that account for imperfect detection.
Grenzverletzungen in Lehrtherapien – Was können wir aus Beschwerden über Schweigepflichtverletzungen lernen?
Authors: Trübel Eva , Schleu Andrea , Ehlers Jan P. 
Published: January 2023

Zusammenfassung
Hintergrund
Während Grenzverletzungen in Psychotherapien in den letzten Jahren zunehmend in den Fokus gerückt sind, waren die Besonderheiten der Grenzverletzungen in Lehrtherapien und Lehranalysen bislang kaum Gegenstand der wissenschaftlichen Untersuchungen.

Ziel
Die vorliegende Arbeit beschreibt spezifische Kontextmerkmale einer Ausbildungssituation, die mit dem Auftreten, dem Verlauf und den Folgen von Schweigepflichtverletzungen verbunden sind.

Methode
Es werden 18 anonymisierte Beratungsdokumentationen des Ethikvereins e. V., in denen Schweigepflichtverletzungen im Rahmen einer Psychotherapie-Ausbildung beobachtet werden, qualitativ im Sinne der „Grounded-theory“-Methodologie, angelehnt an Charmaz, ausgewertet. Dazu wird die Software „MAXQDA“ (von VERBI, Berlin) benutzt.

Ergebnis
Die untersuchten Lehrtherapien hatten schädigende Auswirkungen auf die betroffenen Aus- und Weiterbildungskandidaten und -kandidatinnen (AWT). Die Variablen „Eignungszweifel“, „multiple Rollen des Lehrtherapeuten“ und „institutionelle Verleugnung der Grenzverletzung“ waren bedeutsame ausbildungsspezifische Charakteristika der untersuchten Schweigepflichtverletzungen.
Die Destruktivität der entgleisten dyadischen Beziehung wurde in den ungünstigen Verläufen durch eine Wiederholung der gestörten Beziehung zwischen AWT und dem Institut potenziert.

Schlussfolgerungen
Schweigepflichtverletzungen können schädigende Grenzverletzungen sein, die im Ausbildungskontext auf weitere Grenzverluste in der Dyade und in der Institution hinweisen können. Es werden verschiedene autonomiefördernde Maßnahmen vorgeschlagen, die Ausbildungsteilnehmende vor Grenzverletzungen besser schützen können.
Response of a Canonical Ensemble of Quantum Oscillators to a Random Metric
Authors: Haba Z. 
Published: January 2023

AbstractWe calculate the susceptibility of a canonical ensemble of quantum oscillators to the singular random metric. If the covariance of the metric is $$\vert \textbf{x}-\textbf{x}^{\prime }\vert ^{-4\alpha }$$

|
x
-

x
′

|

-
4
α

($$0<\alpha <\frac{1}{2}$$

0
<
α
<

1
2

)then the expansion of the partition function in powers of the temperature involves non-integer indices.
APC ameliorates idiopathic membranous nephropathy by affecting podocyte apoptosis through the ERK1/2/YB-1/PLA2R1 axis
Authors: Ke Ben , Shen Wen , Liao Yunfei , Hu Jing , Tu Weiping , Fang Xiangdong 
Published: January 2023

AbstractIdiopathic membranous nephropathy (IMN) belongs to an important pathogenic category of adult nephrotic syndrome. PLA2R1 exposure is critical for triggering the pathogenesis of PLA2R1-related IMN. However, the pathogenesis of IMN and the molecular mechanism of treatment remain to be further clarified. The expression changes of activated protein C (APC) and PLA2R1 in IMN patients were quantified by qPCR. A zymosan activated serum (ZAS)-induced IMN podocyte model was established in vitro. Podocyte apoptosis was detected via flow cytometry and caspase‑3 assay. The expression levels of APC, p-ERK1/2, ERK1/2, YB-1 and PLA2R1 were detected by western blotting. The regulation relationship between YB-1 and PLA2R1 was detected by dual fluorescent reporter system. In IMN patients, the expression level of PLA2R1 was increased, whereas the expression level of APC was decreased. When APC was added to podocytes in vitro, the phosphorylation of ERK1/2 was increased, which could promote the translocation of YB-1 to the nucleus that reduces the expression of PLA2R1 at the cellular transcriptional level, thereby inhibiting podocyte apoptosis. Our study is the first to report that APC can improve membranous nephropathy by affecting podocyte apoptosis through the ERK1/2/YB-1/PLA2R1 axis. This study will provide a new targeted therapy for IMN patients with high PLA2R1 expression.
Circuit-Specific Control of Blood Pressure by PNMT-Expressing Nucleus Tractus Solitarii Neurons
Authors: Jun Shirui , Ou Xianhong , Shi Luo , Yu Hongxiao , Deng Tianjiao , Chen Jinting , Nie Xiaojun , Hao Yinchao , Shi Yishuo , Liu Wei , Tian Yanming , Wang Sheng , Yuan Fang 
Published: January 2023

AbstractThe nucleus tractus solitarii (NTS) is one of the morphologically and functionally defined centers that engage in the autonomic regulation of cardiovascular activity. Phenotypically-characterized NTS neurons have been implicated in the differential regulation of blood pressure (BP). Here, we investigated whether phenylethanolamine N-methyltransferase (PNMT)-expressing NTS (NTSPNMT) neurons contribute to the control of BP. We demonstrate that photostimulation of NTSPNMT neurons has variable effects on BP. A depressor response was produced during optogenetic stimulation of NTSPNMT neurons projecting to the paraventricular nucleus of the hypothalamus, lateral parabrachial nucleus, and caudal ventrolateral medulla. Conversely, photostimulation of NTSPNMT neurons projecting to the rostral ventrolateral medulla produced a robust pressor response and bradycardia. In addition, genetic ablation of both NTSPNMT neurons and those projecting to the rostral ventrolateral medulla impaired the arterial baroreflex. Overall, we revealed the neuronal phenotype- and circuit-specific mechanisms underlying the contribution of NTSPNMT neurons to the regulation of BP.
Digitalisation for nuclear waste management: predisposal and disposal
Authors: Kolditz Olaf , Jacques Diederik , Claret Francis , Bertrand Johan , Churakov Sergey V. , Debayle Christophe , Diaconu Daniela , Fuzik Kateryna , Garcia David , Graebling Nico , Grambow Bernd , Holt Erika , Idiart Andrés , Leira Petter , Montoya Vanessa , Niederleithinger Ernst , Olin Markus , Pfingsten Wilfried , Prasianakis Nikolaos I. , Rink Karsten , Samper Javier , Szöke István , Szöke Réka , Theodon Louise , Wendling Jacques 
Published: January 2023

AbstractData science (digitalisation and artificial intelligence) became more than an important facilitator for many domains in fundamental and applied sciences as well as industry and is disrupting the way of research already to a large extent. Originally, data sciences were viewed to be well-suited, especially, for data-intensive applications such as image processing, pattern recognition, etc. In the recent past, particularly, data-driven and physics-inspired machine learning methods have been developed to an extent that they accelerate numerical simulations and became directly usable for applications related to the nuclear waste management cycle. In addition to process-based approaches for creating surrogate models, other disciplines such as virtual reality methods and high-performance computing are leveraging the potential of data sciences more and more. The present challenge is utilising the best models, input data and monitoring information to integrate multi-chemical-physical, coupled processes, multi-scale and probabilistic simulations in Digital Twins (DTw) able to mirror or predict the performance of its corresponding physical twins. Therefore, the main target of the Topical Collection is exploring how the development of DTw can benefit the development of safe, efficient solutions for the pre-disposal and disposal of radioactive waste. A particular challenge for DTw in radioactive waste management is the combination of concepts from geological modelling and underground construction which will be addressed by linking structural and multi-physics/chemistry process models to building or tunnel information models. As for technical systems, engineered structures a variety of DTw approaches already exist, the development of DTw concepts for geological systems poses a particular challenge when taking the complexities (structures and processes) and uncertainties at extremely varying time and spatial scales of subsurface environments into account.
The dark side of the cascading compliance model in global value chains
Authors: Soundararajan Vivek 
Published: January 2023

AbstractIn this paper, I discuss the dark side of the cascading compliance model predominantly used by multinationals to improve working conditions in global value chains. Further, I discuss the origins of such dark side. Finally, I argue for the move from cascading compliance to a shared responsibility model for the improvement of working conditions in global value chains.
Molecular characterization and comparisons of potato wart (Synchytrium endobioticum) in historic collections to recent findings in Canada and the Netherlands
Authors: van de Vossenberg Bart T.L.H. , van Gent Marga , Meffert Johan P. , Nguyen Hai D.T. , Smith Donna , van Kempen Thijn , Helderman Carin M. , Rosendahl-Peters Karin C.H.M. , Braak Naomi te , Flath Kerstin , Przetakiewicz Jarosław , Perez Willmer , Çakir Emel , Sikharulidze Zoia V. , van Leeuwen Gerard C.M. , van der Lee Theo A.J. 
Published: January 2023

AbstractSynchytrium endobioticum (Schilb.) Perc. is a chytrid fungus causing potato wart disease and is one of the most important quarantine diseases on cultivated potato. Infected host tissues develop warts rendering the crop unmarketable. Resting spores, that can remain viable and infectious for decades, are formed in warted tissues and are released into the surrounding soil when host tissue decays.To better understand the pathogen’s diversity and to potentially uncover pathways of migrations and introduction events, molecular characterization was performed on the historical S. endobioticum resting spore collection of the Dutch National Plant Protection Organization. Mitochondrial genomes were assembled and annotated, and four novel structural variants were identified from these materials with intronic presence-absence variation in cox1 or cob genes and structural variation in the dpoB – TIR region. Several fungal isolates were shown to contain mixtures of structural variants. We analyzed the mitogenomic sequences obtained from recent potato wart disease findings in Canada and the Netherlands in the context of the historical materials and found that fungal isolates from the new Dutch outbreak contained a specific mixture of mitogenomic variants previously not observed in the Netherlands. Based on the mitogenomic profile, pathotype 38(Nevşehir) was suspected which was later verified with the Spieckermann bioassay. To further facilitate dissemination of data and interactive visual analytics we created a public Nextstrain webpage with S. endobioticum mitogenomic sequences and associated metadata on their geographic origin, pathotype identity and (mixture) of mitogenomic variants (https://nextstrain.nrcnvwa.nl/Sendo).
Is there still evolution in the human population?
Authors: Kun Ádám 
Published: January 2023

AbstractIt is often claimed that humanity has stopped evolving because modern medicine erased all selection on survival. Even if that would be true, and it is not, there would be other mechanisms of evolution which could still led to changes in allelic frequencies. Here I show, by applying basic evolutionary genetics knowledge, that we expect humanity to evolve. The results from genome sequencing projects have repeatedly affirmed that there are still recent signs of selection in our genomes. I give some examples of such adaptation. Then I briefly discuss what our evolutionary future has in store for us.
Association of Estimated Pulse Wave Velocity with Abdominal Aortic Calcification: A Large Cross-Sectional Study
Authors: Guo Xiaoxu , Xu Chenzhao , Li Yaqiang 
Published: January 2023

Abstract
Objectives
There is evidence that pulse wave velocity (PWV) can predict the occurrence of abdominal aortic calcification (AAC), while the association between estimated PWV (ePWV) and AAC has not been reported, so our study aimed to analyze the association between ePWV and AAC.

Methods
The study enrolled 3140 adults between the ages of 40 and 80 who participated in the 2013–2014 National Health and Nutrition Examination Survey. Using multivariate logistic regression analysis, multivariate linear regression and receiver operating characteristic (ROC) curve to evaluate the association between ePWV and AAC.

Results
The ePWV was significantly higher in participants with AAC compared with those without AAC. And ePWV had a high correlation with age and AAC (correlation coefficient = 0.906 and 0.332, both P < 0.001). Individuals in high ePWV group had significantly higher percentage of AAC compared to low ePWV group (OR = 2.971, 95% CI 2.529–3.490, P < 0.001) in the crude model. After adjusting for all confounding variables, ePWV was still significantly higher (Model 3, OR = 1.962, 95% CI 1.612–2.389, P < 0.001). While after adjusting for all confounding variables plus age (Model 4), ePWV, when as a categorical variable, was no longer significantly positively associated with AAC. Additionally, the ROC curve indicated that both ePWV and age had some diagnostic value for AAC (AUC = 0.690, P < 0.001; AUC = 0.708, P < 0.001).

Conclusions
In the age range of 40–80 years, ePWV did have an association with AAC but did not have predictive power beyond age.
Converging cultures? A comparative analysis of South Korea and Sweden’s national curricula, 1980–2018
Authors: Boman Björn , Mosesson Marcus 
Published: January 2023

AbstractRelating to world culture theory, this article aimed to discern similarities and differences between the South Korean national curriculum (1981, 2015) and the Swedish national curriculum (1980, 2018) and a set of complementary documents, both more broadly and with special emphasis on middle school level mathematics education. The findings suggest that the latest curricula accentuate knowledge and skills. However, Sweden’s national curriculum underlines knowledge and values, while the South Korean counterpart stresses character education and a ‘well-rounded personality’. Mathematics education has not changed much since 1980 in both countries. However, cosmopolitan tendencies are more present in the latest Korean curriculum. Overall, an unequivocal converging effect has not been discerned.
A novel molecular classification method for osteosarcoma based on tumor cell differentiation trajectories
Authors: Zhang Hao , Wang Ting , Gong Haiyi , Jiang Runyi , Zhou Wang , Sun Haitao , Huang Runzhi , Wang Yao , Wu Zhipeng , Xu Wei , Li Zhenxi , Huang Quan , Cai Xiaopan , Lin Zaijun , Hu Jinbo , Jia Qi , Ye Chen , Wei Haifeng , Xiao Jianru 
Published: January 2023

AbstractSubclassification of tumors based on molecular features may facilitate therapeutic choice and increase the response rate of cancer patients. However, the highly complex cell origin involved in osteosarcoma (OS) limits the utility of traditional bulk RNA sequencing for OS subclassification. Single-cell RNA sequencing (scRNA-seq) holds great promise for identifying cell heterogeneity. However, this technique has rarely been used in the study of tumor subclassification. By analyzing scRNA-seq data for six conventional OS and nine cancellous bone (CB) samples, we identified 29 clusters in OS and CB samples and discovered three differentiation trajectories from the cancer stem cell (CSC)-like subset, which allowed us to classify OS samples into three groups. The classification model was further examined using the TARGET dataset. Each subgroup of OS had different prognoses and possible drug sensitivities, and OS cells in the three differentiation branches showed distinct interactions with other clusters in the OS microenvironment. In addition, we verified the classification model through IHC staining in 138 OS samples, revealing a worse prognosis for Group B patients. Furthermore, we describe the novel transcriptional program of CSCs and highlight the activation of EZH2 in CSCs of OS. These findings provide a novel subclassification method based on scRNA-seq and shed new light on the molecular features of CSCs in OS and may serve as valuable references for precision treatment for and therapeutic development in OS.
Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria
Authors: Hurt Robert C. , Buss Marjorie T. , Duan Mengtong , Wong Katie , You Mei Yi , Sawyer Daniel P. , Swift Margaret B. , Dutka Przemysław , Barturen-Larrea Pierina , Mittelstein David R. , Jin Zhiyang , Abedi Mohamad H. , Farhadi Arash , Deshpande Ramya , Shapiro Mikhail G. 
Published: January 2023

AbstractUltrasound allows imaging at a much greater depth than optical methods, but existing genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor sensitivity, specificity and in vivo expression. Here we describe two acoustic reporter genes (ARGs)—one for use in bacteria and one for use in mammalian cells—identified through a phylogenetic screen of candidate gas vesicle gene clusters from diverse bacteria and archaea that provide stronger ultrasound contrast, produce non-linear signals distinguishable from background tissue and have stable long-term expression. Compared to their first-generation counterparts, these improved bacterial and mammalian ARGs produce 9-fold and 38-fold stronger non-linear contrast, respectively. Using these new ARGs, we non-invasively imaged in situ tumor colonization and gene expression in tumor-homing therapeutic bacteria, tracked the progression of tumor gene expression and growth in a mouse model of breast cancer, and performed gene-expression-guided needle biopsies of a genetically mosaic tumor, demonstrating non-invasive access to dynamic biological processes at centimeter depth.
Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Authors: Yang Lei , Machin Frank , Wang Shuangfeng , Saplaoura Eleftheria , Kragler Friedrich 
Published: January 2023

AbstractGeneration of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this issue by designing fusions of Cas9 and guide RNA transcripts to tRNA-like sequence motifs that move RNAs from transgenic rootstocks to grafted wild-type shoots (scions) and achieve heritable gene editing, as demonstrated in wild-type Arabidopsis thaliana and Brassica rapa. The graft-mobile gene editing system enables the production of transgene-free offspring in one generation without the need for transgene elimination, culture recovery and selection, or use of viral editing vectors. We anticipate that using graft-mobile editing systems for transgene-free plant production may be applied to a wide range of breeding programs and crop plants.