bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trapped fourth ventricle: to stent, shunt, or fenestrate—a systematic review and individual patient data meta-analysis
Authors: Sadigh Yasmin , van Surksum Colin , Schröder Philip H. D. , Cozar Ayca , Khandour Dalila , Talbi Lailla , Spoor Jochem K. H. , Eelkman Rooda Oscar H. J. , Volovici Victor , van Veelen Marie-Lise C. 
Published: January 2023

AbstractTrapped or isolated fourth ventricle (TFV) is a rare but critical neurosurgical condition, mostly occurring in pediatric patients, caused by a blockage of the in- and outlets of the fourth ventricle. The purpose of this study is to review all available data on the treatment options of TFV and to compare their safety, efficacy, and durability. MEDLINE, Embase, and Google Scholar were searched from inception to September 13, 2022, for prospective or retrospective cohorts, case-control studies, case series or case reports, reporting detailing outcomes of TFV patients, treated with an endoscopic-, microsurgical-, shunt placement,- or hybrid approaches to TFV. All authors were contacted to provide individual patient data. Eighty-seven articles (314 patients) were included in the individual patient data meta-analysis (IPD) and 9 (151 patients) in the cohort meta-analysis. The IPD revealed that primary endoscopic (aOR 0.21; [95% CI 0.08–0.57]) and microsurgical interventions (aOR 0.21; [95% CI 0.05–0.82]) were associated with a significantly lower revision rate, compared to shunt placement, when adjusted for confounders. Endoscopy was also associated with a significantly higher rate of clinical improvement (aOR 4.56; [95% CI 1.2–18]). The meta-analysis revealed no significant difference in revision rate between the endoscopic (0.33 [95% CI 0.0–0.52]) and shunt group (0.44 [95% CI 0.0–1.0]). Endoscopy should be considered as the first-line treatment of TFV due to its superior efficacy, durability, and similar safety, compared to shunt placement and its minimally invasive nature. Microsurgery should be considered as a second treatment option, due to its similar clinical outcomes and revision rate as endoscopy, but it is more invasive in nature.
Algerian and European hedgehogs cohabiting in periurban environments: spatial behaviour and habitat use
Authors: Gago Héctor , Drechsler Robby M. , Monrós Juan S. 
Published: January 2023

AbstractHabitat fragmentation is one of the most important threats to biodiversity in the last decades. Numerous species are forced to adapt to human presence as urbanization keeps increasing. Some studies show the impact of habitat changes in various species; however, there is little knowledge about the effect of habitat disturbance on hedgehogs in the Iberian Peninsula. We radio tracked 31 male hedgehogs: 14 European hedgehogs and 17 Algerian hedgehogs, being 18 individuals indigenous and 13 translocated. We analysed their home ranges, spatial behaviour and habitat use in a suburban area of Valencia City, Spain, where the two species coexist. Our results show that there are phenologic variations of activity levels, which differ between species, being maximum in spring for the European hedgehog and in summer for the Algerian hedgehog. We did not detect a territorial behaviour or interspecific spatial competition. However, we could detect a clear “release effect”, with translocated individuals presenting increased home range sizes and activity levels as they explore the unknown environment. Furthermore, we found that both species showed a clear preference for the pine forest habitat, avoiding all other habitat types, especially those with high human presence. Our results also suggest that the European hedgehog has a more generalist character than the Algerian hedgehog regarding habitat use. In conclusion, this study highlights the importance of conserving a connected mosaic of green patches in urbanized environments for the conservation of hedgehog populations.
Insights on the role of l-lactate as a signaling molecule in skin aging
Authors: Chirumbolo Salvatore , Bertossi Dario , Magistretti Pierre 
Published: January 2023

Abstractl-lactate is a catabolite from the anaerobic metabolism of glucose, which plays a paramount role as a signaling molecule in various steps of the cell survival. Its activity, as a master tuner of many mechanisms underlying the aging process, for example in the skin, is still presumptive, however its crucial position in the complex cross-talk between mitochondria and the process of cell survival, should suggest that l-lactate may be not a simple waste product but a fine regulator of the aging/survival machinery, probably via mito-hormesis. Actually, emerging evidence is highlighting that ROS are crucial in the signaling of skin health, including mechanisms underlying wound repair, renewal and aging. The ROS, including superoxide anion, hydrogen peroxide, and nitric oxide, play both beneficial and detrimental roles depending upon their levels and cellular microenvironment. Physiological ROS levels are essential for cutaneous health and the wound repair process. Aberrant redox signaling activity drives chronic skin disease in elderly. On the contrary, impaired redox modulation, due to enhanced ROS generation and/or reduced levels of antioxidant defense, suppresses wound healing via promoting lymphatic/vascular endothelial cell apoptosis and death. This review tries to elucidate this issue.
Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells
Authors: Skrika-Alexopoulos Eleftheria , Mark Smales C 
Published: January 2023

AbstractExosomes have previously been isolated from Chinese hamster ovary (CHO) cells and their anti-apoptotic properties reported. However, to further facilitate the study of CHO cell derived exosomes and allow their comparison across studies, it is necessary to characterise and define such exosomes using at least three criteria that can act as a reference for the generation of CHO cell produced exosomes. Here we report on the isolation of exosomes from CHO cells, an industrially relevant and widely used cell host for biopharmaceutical protein production, during the exponential and stationary phase of growth during batch culture using a Total Exosome Isolation (TEI) method. The resulting vesicles were characterized and visualized using a diverse range of techniques including Dynamic Light Scattering (DLS), Zeta potential, Electron Microscopy and immunoblotting, and their protein and RNA content determined. We also generated the lipid fingerprint of isolated exosomes using MALDI-ToF mass spectroscopy. We confirmed the presence of nano sized extracellular vesicles from CHO cells and their subsequent characterization revealed details of their size, homogeneity, surface charge, protein and RNA content. The lipid content of exosomes was also found to differ between exosomes isolated on different days of batch culture. This analysis provides a profile and characterisation of CHO cell exosomes to aid future studies on exosomes from CHO cells and improving the manufacturing of exosomes for biotherapeutic application.
The cohesin modifier ESCO2 is stable during DNA replication
Authors: Jevitt Allison M. , Rankin Brooke D. , Chen Jingrong , Rankin Susannah 
Published: January 2023

AbstractCohesion between sister chromatids by the cohesin protein complex ensures accurate chromosome segregation and enables recombinational DNA repair. Sister chromatid cohesion is promoted by acetylation of the SMC3 subunit of cohesin by the ESCO2 acetyltransferase, inhibiting cohesin release from chromatin. The interaction of ESCO2 with the DNA replication machinery, in part through PCNA-interacting protein (PIP) motifs in ESCO2, is required for full cohesion establishment. Recent reports have suggested that Cul4-dependent degradation regulates the level of ESCO2 protein following replication. To follow up on these observations, we have characterized ESCO2 stability in Xenopus egg extracts, a cell-free system that recapitulates cohesion establishment in vitro. We found that ESCO2 was stable during DNA replication in this system. Indeed, further challenging the system by inducing DNA damage signaling or increasing the number of nuclei undergoing DNA replication had no significant impact on the stability of ESCO2. In transgenic somatic cell lines, we also did not see evidence of GFP-ESCO2 degradation during S phase of the cell cycle using both flow cytometry and live-cell imaging. We conclude that ESCO2 is stable during DNA replication in both embryonic and somatic cells.
Development and Validation of a Theory-Based Questionnaire to Measure Different Types of Cognitive Load
Authors: Krieglstein Felix , Beege Maik , Rey Günter Daniel , Sanchez-Stockhammer Christina , Schneider Sascha 
Published: January 2023

AbstractAccording to cognitive load theory, learning can only be successful when instructional materials and procedures are designed in accordance with human cognitive architecture. In this context, one of the biggest challenges is the accurate measurement of the different cognitive load types as these are associated with various activities during learning. Building on psychometric limitations of currently available questionnaires, a new instrument for measuring the three types of cognitive load—intrinsic, extraneous, and germane cognitive load—is developed and validated relying on a set of five empirical studies. In Study 1, a principal component analysis revealed a three-component model which was subsequently confirmed using a confirmatory factor analysis (Study 2). Finally, across three experiments (Studies 3–5), the questionnaire was shown to be sensitive to changes in cognitive load supporting its predictive validity. The quality of the cognitive load questionnaire was underlined by satisfactory internal consistencies across all studies. In sum, the proposed questionnaire can be used in experimental settings to measure the different types of cognitive load in a valid and reliable manner. The construction and validation process of the questionnaire has also shown that the construct germane cognitive load remains controversial concerning its measurement and theoretical embedding in cognitive load theory.
COVID-19 and comedications in atrial fibrillation—a case–control study in Stockholm
Authors: Bell Max , Ekbom Anders , Linder Marie 
Published: January 2023

AbstractTo test the main hypothesis that anticoagulation reduces risk of hospitalization, intensive care unit (ICU) admission and death in COVID-19. Nested case–control study among patients with atrial fibrillation (AF) in Stockholm. COVID-19 cases were matched to five disease-free controls with same sex, born within ± 1 years. Source population was individuals in Stockholm with AF 1997–2020. Swedish regional and national registers are used. National registers cover hospitals and outpatient clinics, local registers cover primary care. Records were linked through the personal identity number assigned to each Swedish resident. Cases were individuals with COVID-19 (diagnosis, ICU admission, or death). The AF source population consisted of 179,381 individuals from which 7548 cases were identified together with 37,145 controls. The number of cases (controls) identified from hospitalization, ICU admission or death were 5916 (29,035), 160 (750) and 1472 (7,360). The proportion of women was 40% for hospitalization and death, but 20% and 30% for admission to ICU in wave one and two, respectively. Primary outcome was mortality, secondary outcome was hospitalization, tertiary outcome was ICU admission, all with COVID-19. Odds ratios (95% confidence interval) for antithrombotics were 0.79 (0.66–0.95) for the first wave and 0.80 (0.64–1.01) for the second wave. Use of anticoagulation among patients with arrythmias infected with COVID-19 is associated with lower risk of hospitalization and death. If further COVID-variants emerge, or other infections with prothrombotic properties, this emphasize need for physicians to ensure compliance among vulnerable patients.
Conformal Cyclic Cosmology, gravitational entropy and quantum information
Authors: Eckstein Michał 
Published: January 2023

AbstractWe inspect the basic ideas underlying Roger Penrose’s Conformal Cyclic Cosmology from the perspective of modern quantum information. We show that the assumed loss of degrees of freedom in black holes is not compatible with the quantum notion of entropy. We propose a unitary version of Conformal Cyclic Cosmology, in which quantum information is globally preserved during the entire evolution of our universe, and across the crossover surface to the subsequent aeon. Our analysis suggests that entanglement with specific quantum gravitational degrees of freedom might be at the origin of the second law of thermodynamics and the quantum-to-classical transition at mesoscopic scales.
Prioritising Children and Young People with Disability in Research About Domestic and Family Violence: Methodological, Ethical and Pragmatic Reflections
Authors: Robinson Sally , Foley Kristen , Moore Tim , Valentine Kylie , Burton Jala , Marshall Amy , O’Donnell Melissa , Brebner Chris 
Published: January 2023

Abstract
Purpose
The perspectives of children and young people with disability who experience domestic and family violence are under-researched, impeding the development of approaches that meet their needs. Knowledge gaps stem from the layered discursive positioning of disability, childhood/youth, or domestic and family violence in addition to the methodological, ethical and pragmatic complexity of research needed to understand their priorities and be attuned to their lived experience. This article explores methodological, ethical and practical challenges to centring their voices in research about domestic and family violence.

Method
A conceptual framework of feminist disability theory and intersectionality informed our co-designed research, across three phases: (1) quantitative large-scale data linkage and case file analysis; (2) qualitative research with children and young people, their families and service providers and (3) stakeholder engagement workshops.

Results
We reflect on how our research was able to prioritise the contextual agency of children and young people with disability, ways it could not, and other constraints.

Conclusion
Children and young people with disability experiencing domestic and family violence hold an expert and unique vantage point on what happens to them. Amplifying their priorities for directing policy and organisational change requires more of researchers in terms of methods, but also more flexibility in how projects are funded to enable creativity and innovation. We call for collective attention to frameworks for supported decision-making and child ethics to progress inclusive research which recognises the importance of participation for children and young people with disability.
Investigating non-inferiority or equivalence in time-to-event data under non-proportional hazards
Authors: Möllenhoff Kathrin , Tresch Achim 
Published: January 2023

AbstractThe classical approach to analyze time-to-event data, e.g. in clinical trials, is to fit Kaplan–Meier curves yielding the treatment effect as the hazard ratio between treatment groups. Afterwards, a log-rank test is commonly performed to investigate whether there is a difference in survival or, depending on additional covariates, a Cox proportional hazard model is used. However, in numerous trials these approaches fail due to the presence of non-proportional hazards, resulting in difficulties of interpreting the hazard ratio and a loss of power. When considering equivalence or non-inferiority trials, the commonly performed log-rank based tests are similarly affected by a violation of this assumption. Here we propose a parametric framework to assess equivalence or non-inferiority for survival data. We derive pointwise confidence bands for both, the hazard ratio and the difference of the survival curves. Further we propose a test procedure addressing non-inferiority and equivalence by directly comparing the survival functions at certain time points or over an entire range of time. Once the model’s suitability is proven the method provides a noticeable power benefit, irrespectively of the shape of the hazard ratio. On the other hand, model selection should be carried out carefully as misspecification may cause type I error inflation in some situations. We investigate the robustness and demonstrate the advantages and disadvantages of the proposed methods by means of a simulation study. Finally, we demonstrate the validity of the methods by a clinical trial example.
American Community Survey (ACS) Data Uncertainty and the Analysis of Segregation Dynamics
Authors: Wei Ran , Knaap Elijah , Rey Sergio 
Published: January 2023

AbstractAmerican Community Survey (ACS) data have become the workhorse for the empirical analysis of segregation in the U.S.A. during the past decade. The increased frequency the ACS offers over the 10-year Census, which is the main reason for its popularity, comes with an increased level of uncertainty in the published estimates due to the reduced sampling ratio of ACS (1:40 households) relative to the Census (1:6 households). This paper introduces a new approach to integrate ACS data uncertainty into the analysis of segregation. Our method relies on variance replicate estimates for the 5-year ACS and advances over existing approaches by explicitly taking into account the covariance between ACS estimates when developing sampling distributions for segregation indices. We illustrate our approach with a study of comparative segregation dynamics for 29 metropolitan statistical areas in California, using the 2010–2014 and 2015–2019. Our methods yield different results than the simulation technique described by Napierala and Denton (Demography 54(1):285–309, 2017). Taking the ACS estimate covariance into account yields larger error margins than those generated with the simulated approach when the number of census tracts is large and minority percentage is low, and the converse is true when the number of census tracts is small and minority percentage is high.
Does it pay off to offer family-friendly practices? Exploring the missing links to performance
Authors: Guedes Maria João , Soares Maria Eduarda , Mosquera Pilar , Borregana João 
Published: January 2023

AbstractFamily-friendly practices (FFP) are viewed as an important tool to achieve equal opportunities and a supportive culture, with potential benefits for the organizational image and its employer branding. To date, however, there is an imperfect understanding of whether FFP affect firm performance and what are the mechanism of the possible association. This study tests a comprehensive model to investigate whether FFP affect firm performance and analyzes the role played by work–life balance (WLB) as a mediator between the provision of FFP and employee attitudes and firm performance.Using a sample of 724 public and private Portuguese companies and Partial Least Squares (PLS) regression, the results show that the availability of FFP indeed has a positive influence on WLB, organizational commitment, and job satisfaction, but it does not impact family satisfaction. Moreover, WLB mediates the relationship between FFP and firm performance. It is noteworthy that men and women do not perceive the impact of these practices the same way, with FFP having a greater impact on female job satisfaction than on their male counterparts. In addition, participants with lower levels of education and lower levels of annual income perceive a stronger impact of FFP on organizational commitment and WLB than the remaining participants. Men and participants with lower levels of education also perceive a stronger impact of WLB on family satisfaction.
Losses never sleep – The effect of tax loss offset on stock market returns during economic crises
Authors: Koch Reinald , Holtmann Svea , Giese Henning 
Published: January 2023

AbstractWe analyze to what extent more generous tax loss offset regulations are associated with a weaker decline and stronger recovery of firm stock prices during economic crises. We argue that an unrestricted loss carryforward and, particularly, an unrestricted loss carryback provides firms with additional liquidity, which should lower the risk of bankruptcy and can be used for investment purposes. Our empirical findings document that (1) an unrestricted loss carryforward and an unrestricted loss carryback result in a weaker decline and more timely recovery of stock prices during the considered crises, (2) this effect is stronger in high-tax countries, and (3) this effect is also dependent upon pre-crisis profitability.
Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference
Authors: Czarnek Maria , Kochan Jakub , Wawro Mateusz , Myrczek Rafał , Bereta Joanna 
Published: January 2023

AbstractIn recent years, CRISPR interference (CRISPRi) technology of gene silencing has emerged as a promising alternative to RNA interference (RNAi) surpassing the latter in terms of efficiency and accuracy. Here, we describe the construction of a set of transposon vectors suitable for constitutive or tetracycline (doxycycline)-inducible silencing of genes of interest via CRISPRi method and conferring three different antibiotic resistances, using vectors available via Addgene repository. We have analyzed the performance of the new vectors in the silencing of mouse Adam10 and human lncRNA, NORAD. The empty vector variants can be used to efficiently silence any genes of interest.
Polymorphic Alpha-Synuclein Oligomers: Characterization and Differential Detection with Novel Corresponding Antibodies
Authors: Moore Kenya , Sengupta Urmi , Puangmalai Nicha , Bhatt Nemil , Kayed Rakez 
Published: January 2023

Abstract
The pathological hallmark of many neurodegenerative diseases is the accumulation of characteristic proteinaceous aggregates. Parkinson’s disease and dementia with Lewy bodies can be characterized as synucleinopathies due to the abnormal accumulation of the protein alpha-synuclein (α-Syn). Studies have shown amyloidogenic proteins such as α-Syn and tau can exist as polymorphic aggregates, a theory widely studied mostly in their fibrillar morphology. It is now well understood that an intermediate state of aggregates, oligomers, are the most toxic species. We have shown α-Syn, when modified by different physiological inducers, result in distinct oligomeric conformations of α-Syn. Polymorphic α-Syn oligomers exhibit distinct properties such as aggregate size, conformation, and differentially interact with tau. In this study, we confirm α-Syn oligomeric polymorphs furthermore using in-house novel α-Syn toxic conformation monoclonal antibodies (SynTCs). It is unclear the biological relevance of α-Syn oligomeric polymorphisms. Utilizing a combination of biochemical, biophysical, and cell-based assays, we characterize α-Syn oligomeric polymorphs. We found α-Syn oligomeric polymorphs exhibit distinct immunoreactivity and SynTCs exhibit differential selectivity and binding affinity for α-Syn species. Isothermal titration calorimetry experiments suggest distinct α-Syn:SynTC binding enthalpies in a species-specific manner. Additionally, we found SynTCs differentially reduce α-Syn oligomeric polymorph-mediated neurotoxicity and propagation in primary cortical neurons in a polymorph-specific manner. These studies demonstrate the biological significance of polymorphic α-Syn oligomers along with the importance of polymorph-specific antibodies that target toxic α-Syn aggregates. Monoclonal antibodies that can target the conformational heterogeneity of α-Syn oligomeric species and reduce their mediated toxicity have promising immunotherapeutic potential.