bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D plasmonic SERS aptasensor for rapid detection of aflatoxin B1 combined with Au@Ag bimetallic nanostars and Fe3O4 @MoS2 magnetic nanoflowers
Authors: Chen Peifang , Li Chenbiao , Ma Xiaoyuan , Wang Zhouping , Jiang Caiyun 
Published: January 2023

Abstract
As a virulent metabolite, aflatoxin B1 (AFB1) presented in various cereal grain is tightly implicated in severe human diseases. In this study, 3D plasmonic nanohybirds of Raman molecule 4-mercaptobenzoic acid (4-MBA)-embedded and AFB1 aptamer-modified bimetallic nanostars as probes bound to magnetic nanoflowers were fabricated and demonstrated as a high-performance SERS-active aptasensor to quantitatively analyze AFB1. Bimetallic Au@Ag SERS plasmonic nanoprobes with enhanced properties were capable of enhancing discriminative Raman peaks of 4-MBA. Then, the integration of iron tetroxide nanoparticles (Fe3O4 NPs) and molybdenum disulfide nanosheets (MoS2 NSs) with huge specific surface area constituted stable 3D Fe3O4@MoS2 plasmonic nanoflowers, facilitating the bind of numerous aptamer-based SERS probes via the non-covalent interaction between MoS2 NSs and aptamer, which were ideal candidates for SERS-active substrates. Additionally, Fe3O4 NPs as magenetic core endowed 3D nanocomposites with specific magnetic separation characteristic that caused the collected SERS hotspots to exhibit superior signal response, and further strengthening the sensitivity in a complex food matrix. Aptamer-target AFB1 specific recognition triggered linearly diminished 4-MBA signal intensity (I4 − MBA) on the substrate to achieve a low detection limit of 58.9 pg/mL. Furthermore, the sensor has the potential to be a promising monitoring tool for trace contaminants.
Long non-coding RNA ZFHX4-AS1 Upregulates the Neural Differentiation-related Gene ZFHX4 and thereby Promotes SOX2 Expression to Accelerate Glioma Progression
Authors: Yan Hongshan , Shi Yunpeng , Jin Qianxu , Guo Kai , Zhu Siyu , Zhang Shiyang , Zhao Zongmao 
Published: January 2023

Abstract
Background
ZFHX4-AS1 is a recently discovered long noncoding RNA, and whether it is involved in glioma progression is unclear.
Results
We found that ZFHX4-AS1 and its nearby gene ZFHX4 were highly expressed in gliomas through analysis of online databases combined with experimental validation of collected glioma tissues and cells. In functional experiments, we found that high expression levels of ZFHX4-AS1 or ZFHX4 stimulated the proliferation, invasion and migration of glioma cells. Furthermore, overexpression and silencing of ZFHX4-AS1 resulted in the same changes in ZFHX4 expression levels. Combined with rescue experiments, we concluded that ZFHX4-AS1 can affect glioma progression by regulating ZFHX4. Based on the differentially expressed genes and related gene analysis of ZFHX4, we hypothesized that SOX2 was highly correlated with ZFHX4. Subsequently, we confirmed that SOX2 was highly expressed in glioma tissues and cells and was able to bind to ZFHX4. In functional experiments, we demonstrated that highly expressed SOX2 promotes glioma cells invasion and migration. By analyzing the differentially expressed genes of SOX2, we found that the functional node enriched for this differential gene is roughly the same as that of ZFHX4. It appears that these two genes synergize with one another to promote glioma cells progression.Afterwards, SOX2 activates the JAK1/STAT3 pathway and ZFHX4-AS1 reverses the SOX2-induced changes.
Conclusions
In conclusion, ZFHX4-AS1 upregulates the neighboring gene ZFHX4, which in turn increases the expression of SOX2 and stimulates glioma cells proliferation, invasion and migration.
Self-assessment of Doctor-patient Communication Skills of Chinese Medical Students: A cross-sectional study
Authors: Guo Shi , Peng Hui , Xu Dejun , Xu Penghui , Lu Jingjing 
Published: January 2023

Abstract

Background: Effective communication between doctors and patients can contribute to a positive therapeutic effect, better patient outcomes and satisfaction. Doctor-patient communication skills are essential skills that all medical students should acquire during their clinical training. This study aimed to explore the doctors-patient communication skills of medical students in China and the potential influence factors.
Methods: A cross-sectional study was conducted in WuHu City, China. Our study was a part of the teaching quality evaluation of Wannan Medical College. Medical students who were in their final year of clinical learning and have finished a series of pre-clinical training were invited to participate in the survey. 607 medical students participated in this study. Students' social demographic, work-related characteristics and doctor-patient communication skills were collected. The doctor-patient communication skills were measured using the doctor-patient Communication Behavior Scale. The influencing factors were explored by univariate analysis and multiple linear regression analysis.
Results: Compared with the reference population, medical students reported significantly higher scores in total scores for the doctor-patient Communication Behavior Scale for Medical Students.Medical students without doctor-patient communication experiences (β=-11.87, 95%CI=-17.49,-6.24, p<0.01), or without training (β=-3.72, 95%CI=-6.61,-0.83, p<0.05) reported significantly lower total scores.
Conclusion: To further promote students’ doctor-patient communication skills, medical universities and colleges should offer students with more practice opportunities and corresponding training, and communication education should run throughout the whole process of medical education.
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector
Authors: Mo Qingrong , Wang Hao , He Wei , Lin Siyuan , Xie Xin , Wang Yuxu , Wang Xindong , Ren Tongwei , Ouyang Kang , Chen Ying , Huang Weijian , Wei Zuzhang 
Published: January 2023

Abstract
The mechanism of discontinuous transcription for the synthesis of a series of sub-genomic mRNAs to express the porcine reproductive and respiratory syndrome virus (PRRSV) structural proteins potentially allows for the simultaneous expression of multiple foreign genes. This can occur by insertion of multiple novel independent transcription units between ORF sequences of the PRRSV genome. Here, an expression cassette consisting of a red fluorescent protein (RFP) gene flanked at its 3′ end by transcription-regulating sequences (TRS) and an expression cassette consisting of an iLOV gene flanked at its 5′ end by TRS was constructed. The resulting expression cassette containing a RFP gene and containing iLOV gene was introduced between ORF1b and 2, and between ORF7 and 3′UTR, respectively, in an infectious PRRSV cDNA clone. Transfection of the resulting clone (pGX-12RFP-73iLOV) into cells resulted in the recovery of a recombinant virus (rGX-12RFP-73iLOV). Simultaneous expression of RFP and iLOV was observed in MARC-145 cells infected with rGX-RFP-iLOV. To test the ability of the PRRSV genome to express three reporter genes simultaneously, an expression cassette containing the Gluc gene and an expression cassette containing iLOV gene were also inserted in between ORF1b and 2, and between ORF7 and 3′UTR, respectively. This was performed in a recently obtained infectious PRRSV cDNA clone carrying a RFP gene in nsp2. Transfection of the construct (pGX-R-Gluc-iLOV) carrying three reporter genes into cells allowed the rescue of the recombinant reporter virus (rGX-R-Gluc-iLOV) which showed similar growth characteristics to the parental virus and yet yielded 100-fold less infectious viruses. Fluorescence microscopy of cells infected with rGX-R-Gluc-iLOV demonstrated the presence of both GFP and iLOV genes. Gluc activities in supernatants harvested at different time points from cells infected with recombinant viruses carrying Gluc showed the levels of Gluc activity increased as the infection progressed, indicating that the expression of Gluc gene and its activity were parameters for monitoring viral propagation. These results indicate that it is possible to introduce at least three foreign proteins simultaneously in a PRRSV-based vector and this will prove invaluable in our future understanding of these viruses.
In-phase and anti-phase bursting dynamics and synchronisation scenario in neural network by varying coupling phase
Authors: Thazhathethil Remi , Abdulraheem Subha Pallimanhiyil 
Published: January 2023

Abstract
We have analysed the phase flip in the membrane potential and the subsequent emergence of patterns in a network of Hindmarsh Rose neurons under the influence of self, mixed and cross coupling of state variables. The interactions are realised using rotation matrix and the toggle among self, mixed and cross coupling modes are carried out by varying the coupling phase. With the increase in number of variables having self coupling, the value of coupling strength at which synchrony is obtained, decreases drastically. The activator self or cross coupling in membrane potential induces in-phase synchrony whereas the inhibitor coupling induces anti-phase bursts. Due to the interplay of non local coupling and rotation symmetry breaking by crosscoupling of state variables, chimera, multi chimera and traveling chimera states are obtained in the N coupled network. We found that self coupling in multiple variables itself could induce symmetry break in the network proving that cross coupling is not a prerequisite for obtaining chimera. The strength of incoherence and discontinuity measure validates the existence of chimera and multichimera states. The inhibitor self coupling in local interaction induces interesting patterns like Mixed Oscillatory State and clusters. This study of the transition mechanism between in-phase and anti-phase oscillations may help in understanding the realistic spatiotemporal communications of brain.
PACS 05.45.-a · 05.45.Xt
A strategy to prevent post-endoscopic retrograde cholangiopancreatography bleeding in end-stage renal disease patients
Authors: Wang Sheng-Fu , Wu Chi-Huan , Lee Mu-Hsien , Tsou Yung-Kuan , Lin Cheng-Hui , Liu Nai-Jen , Yeh Chau-Ting , Wang Yu-Ching , Sung Kai-Feng 
Published: January 2023

Abstract
Background:
End-stage renal disease (ESRD) is a risk factor of post-endoscopic retrograde cholangiopancreatography (ERCP) bleeding, which may prolong hospitalization or increase the mortality rate. Hemodialysis is known to correct platelet dysfunction from ESRD but whether it can decrease post-ERCP bleeding is still uncertain. Endoscopic papillary balloon dilation (EPBD) had been reported to decrease post-ERCP bleeding but lacks evidence on whether replacing endoscopic sphincterotomy (EST).
Aims:
To explore the predictive factors of post-ERCP bleeding and if hemodialysis could decrease post-ERCP bleeding in ESRD patients. Furthermore, to investigate if the post ERCP bleeding rate, the severity of hemorrhage, and difficulty of treatment differ between EST and EPBD.
Materials and Methods:
This retrospective study acquired patient data from Chang Gung Memorial Hospitals by using Chang Gung Research Database (CGRD) from January 1, 2010, to June 30, 2020. We enrolled ESRD patients who underwent EST or EPBD on the naïve major papilla. First, we analyze the protective factors to prevent post-ERCP bleeding. And to compare the severity of post-ERCP bleeding and difficulty of hemostatic treatment between EST or EPBD with bleeding.
Results:
Total 580 patients enrolled, and we found hemodialysis significantly decreased post ERCP bleeding in the group of eGFR < 15 mL / min / 1.73m2 (p = 0.039, OR = 0.65, 95% CI: 0.43–0.98). Although the bleeding incidence was similar between EST and EPBD groups, there were significantly more patients in the EST bleeding group who required invasive hemostasis procedures (p = 0.001, N = 30/41, 73.17%). Patients who required two or more hemostasis procedures, such as trans-arterial embolization or endoscopic hemostasis, were exclusively in the EST bleeding group.
Conclusion:
Hemodialysis can decrease post ERCP bleeding rate in patients with eGFR < 15 mL / min / 1.73m2. Although the bleeding incidence is similar between EST and EPBD in ESRD patients, hemostatic treatments were more complicated in EST with bleeding than in EPBD.
Barriers to Help-seeking in Medical Students With Anxiety at the University of South Carolina School of Medicine Greenville
Authors: Kaiser Helen , Grice Tori , Walker Brittany , Kaiser Jacob 
Published: January 2023

Abstract
Anxiety levels in medical students have been reported as higher than the aged-matched general population, yet medical students are less likely to seek care for mental health issues. Medical students carry high levels of self-stigma about their own mental health and fear the negative consequences of seeking care. The purpose of this study was to examine the student population at the University of South Carolina School of Medicine Greenville (UofSC SOMG) for anxiety levels and determine the self-stigma attitudes this population carries. UofSC SOMG students were surveyed using the GAD7, questions about mental health stigma, and open-ended questions on barriers to mental health care in medical students. Anxiety levels were compared to student responses. 31% of students reported moderate-severe anxiety levels. Stigma was the most frequently listed barrier to care, however, students with moderate-severe anxiety were more likely to report cost as a barrier to care than students with minimal anxiety levels. Despite free and accessible mental health care, medical students at UofSC SOMG still have anxiety at rates higher than the general population. Future work should help to provide interventions to the barriers of care, so medical students can better utilize mental health care resources.
Nickelalumite, ideally NiAl4(SO4)(OH)12(H2O)3 , a new-old mineral from the Kara-Tangi uranium deposit, Kyrgyzstan
Authors: Karpenko Vladimir Yu. , Agakhanov Atali A. , Pautov Leonid A. , Bekenova Galiya K. , Uvarova Yulia A. , Sokolova Elena , Dikaya Tamara V. , Hawthorne Frank C. 
Published: January 2023

Abstract
Nickelalumite, ideally NiAl4(SO4)(OH)12(H2O)3, is a newly approved mineral from the Batken region, Kyrgyzstan, where it occurs in the Kara-Tangi and Kara-Chagyr uranium deposits. It is found in the zone of hydrothermal alteration of U–V-bearing carbon-rich silicified schists, in association with quartz, calcite, alumohydrocalcite, allophane, crandallite, kyrgyzstanite, ankinovichite and an unknown Al–OH-mineral. Nickelalumite formed by hydrothermal alteration of U–V bearing carbon-rich silicified schists. It occurs as aggregates of colourless to pistachio-green radiating bladed crystals from 0.05 to 0.50 mm long. It is vitreous to transparent in thin flakes, has a white streak, and shows no fluorescence under long-wave or short-wave ultraviolet light. Cleavage is perfect parallel to {001} and no parting was observed. Mohs hardness is 2, it is brittle and has a splintery fracture. The calculated density is 2.231 g.cm–3. In transmitted plane-polarized white light, nickelalumite is non-pleochroic, biaxial, α = 1.542(2), γ = 1.533(2), β could not be measured due to the almost negligible thickness of the flakes. Electron-microprobe analysis gave Al2O3 39.94, SiO2 0.17, SO2 12.16, V2O3 0.29, FeO 0.15, NiO 8.00, ZnO 6.21, (H2O)calc. 31.87, sum 98.79 wt%, H2O was determined by crystal-structure analysis, and the empirical formula is as follows: (Ni0.55Zn0.39V0.02Fe0.01)Σ0.97(Al3.99Si0.01)Σ4.00 (SO4)(OH)12(H2O)3 based on 4 (Al + Si) cations. There is considerable variation in substitution of Zn, Cu and Fe for Ni and V for S. The crystal structure of nickelalumite was refined to an R1 index of 5.66% and consists of interrupted [NiAl4(OH)12] sheets intercalated with layers of {(SO4)2(H2O)3}; nickelalumite is a member of the chalcoalumite group.
Interleukin-5 deficiency promotes macrophage differentiation and exacerbates cardiac remodeling via the STAT3 pathway in angiotensin II-infused mice Running title: IL-5 alleviates cardiac remodeling
Authors: Shen Caijie , Wu Nan , Peng Jianye , Feng Mingjun , Yu Yibo , Wang Jian , Chen Xiaomin 
Published: January 2023

Abstract
Interleukin-5 (IL-5) was reported to participate in cardiovascular diseases, this study aimed to investigate the effects of IL-5 on cardiac remodeling. Mice were infused with angiotensin II (Ang II), and we found cardiac IL-5 expression was time- and dose-dependently decreased after Ang II infusion, and was mainly derived from cardiac macrophages. Additionally, the effects of IL-5 knockout on Ang II-induced cardiac remodeling were observed, and the results exhibited that IL-5 deficiency significantly increased the expression of cardiac hypertrophy markers, elevated myocardial cell cross-sectional areas, and worsened cardiac dysfunction in Ang II-infused mice. IL-5 deletion also promoted M2 macrophage differentiation and exacerbated cardiac fibrosis. Effects of IL-5 on cardiac remodeling and M2 macrophage differentiation were reversed by S31-201. IL-5 knockout significantly increased the Ang II-induced mRNA expression of cardiac hypertrophy markers in myocardial cells that were co-cultured with macrophages, and this effect was also reversed by S31-201. Similar trends in the mRNA levels of fibrosis markers were observed when cardiac fibroblasts and macrophages were co-cultured. Our study suggests that IL-5 deficiency promotes M2 macrophages differentiation and exacerbating cardiac remodeling by activating the STAT3 pathway in Ang II-infused mice. IL-5 may be a potential target for the clinical prevention of cardiac remodeling.
Assessment for bone health in patients with differentiated thyroid carcinoma after postoperative TSH suppression therapy: A new Fracture Risk Assessment Algorithm
Authors: Jia Huiran , Qu Wei , cai Xiaoting , Li Meiye , Qian Ying , Jiang Zhaoshun , Zhang Zongjing 
Published: January 2023

Abstract
Purpose In the general population, the Fracture Risk Assessment tool (FRAX) is used to assess the 10-year risk of major site and hip fractures; however, it is unclear whether this tool can be applied to patients receiving levothyroxine-based Thyroid Stimulating Hormone (TSH) suppressive therapy for postoperative differentiated thyroid cancer patients.
Methods Sixty-four postoperative differentiated thyroid cancer patients and 30 gender-and-age-matched controls were selected. The FRAX was used to calculate the fracture risk with and without bone mineral density (BMD). Thyroid function include TSH were detected and then TSH level were converted to an age-weighted score to calculate the fractures risk of postoperative differentiated thyroid cancer patients. Dual-energy X-ray bone mineral density measurement T score was used as the gold standard to diagnose osteoporosis. SPSS 26.0 software was used for statistical analysis.
Results The T score of bone mineral density in the disease group was lower than that in the control group (-1.09 vs -1.36, P<0.05). The area under the curve of FRAX without BMD for predicting major osteoporotic fractures (PMOF) and hip fractures (PHF) was 0.694 and 0.683, respectively. The cut-off values were 2.15% and 0.25%, respectively. The area under the curve of FRAX with BMD for PMOF and PHF was 0.976 and 0.989, respectively, and the cut-off values were 4.15% and 1.1%, respectively. The area under the curve of FRAX without BMD for PMOF and PHF was 0.708 and 0.72 respectively, and the cut-off values were 5.5% and 1.55%, respectively.
Conclusions The FRAX is suitable for postoperative differentiated thyroid cancer patients after TSH suppressive therapy. In the absence of BMD, TSH weighted by age can improve the specificity of FRAX in the diagnosis of osteoporosis in this population.
Burnout and Quality of Life in Cancer Palliative Care - a Preliminary Study
Authors: Gonçalves Florbela , Gaudêncio Margarida 
Published: January 2023

Abstract
Background: Palliative care is an approach that improves the quality of life of patients and their families who are facing challenges associated with life-threatening illness, through the prevention and relief of suffering. Palliative care health professionals are considered a risk group for the development of burnout, since they live with severe disease and death, on a daily basis. With this work, the authors intend to evaluate the quality of life and risk of burnout in a group of health professionals, who work in a tertiary hospital dedicated to cancer patients.
Material and methods: The authors conducted a quantitative, descriptive, correlational and transversal study on palliative care professionals working with cancer patients. The evaluation protocol used to collect data included a sociodemographic questionnaire, WHO Quality of life Assessment instrument and Maslach Burnout Inventory. Statistical analysis was performed using the SPSS®Statistics program (version 21.0 for Windows®, IBM®). In all statistical evaluations, a p value of 0,05 or less was considered statistically significant.
Results: In the sample, there is a predominance of female gender (79,4%) with a mean age of 43,2 +/- 10,8 years. The most representative professional group was nursing (47,1%). Analyzing Maslach Burnout Inventory score, it appears that physicians and nurses have higher levels of exhaustion when compared to the other groups. In relation to quality of life (QoL), it was observed that in all dimensions, there was a homogeneous distribution of responses. It was verified that it was not possible to establish any relationship between the dimensions of burnout and QoL. Thus, the various dimensions behaved independently.
Discussion and conclusions: Physicians and nurses had the highest burnout levels in the most dimensions of Burnout score, in which they were followed by the operational assistants, who had moderate scores. Despite hight prevalence of Burnout, there is no correlation between Burnout and quality of life in this population. The perception of QoL is very satisfactory in the sample studied may result from the fact that these individuals have developed adequate self-protection strategies, thus preventing QoL from being affected by Burnout.
Synthesis and molecular docking study of new thiazolidinones incorporating a benzoate moiety as anti-HepG2 cancer agents, EGFR inhibitors and apoptosis inducers
Authors: Eldaly Salwa Magdy , Zakaria Dalia Salama , Metwally Nadia Hanafy 
Published: January 2023

Abstract
Synthesis of new anticancer candidates with protein kinases inhibitory potency is a major goal of pharmaceutical science and synthetic research. This current work represents the synthesis of a series of substituted thiazolidinones incorporating a benzoate moiety, starting from 4-formylphenyl benzoate 1a and 4-formyl-2-methoxyphenyl benzoate 1b. Most prepared thiazolidinones 5a-j, 7a-h and 9a-j, were evaluated in vitro for their potential anticancer activity against three cell lines (HepG2, MCF-7 and HeLa). The most active cytotoxic compounds 3a, 3b, 5a, 5c and 5h were then further tested against the normal cell line WI38. All of these were shown be more effective toward anticancer cell lines. Thiazolidinones 5c and 5h were further evaluated to be kinase inhibitors against EGFR showing effective inhibitory impact. Furthermore, 5c and 5h were tested for their effects on cell cycle and apoptosis induction capability in HepG2 cell lines by DNA-flow cytometry analysis and annexin V-FITC apoptosis assay, respectively. The results showed that they have effect of disrupting the cell cycle and causing cell mortality by apoptosis in the treated cells. Moreover, molecular docking studies by the Moe 2015 program showed better binding patterns for 5c and 5hwith the active site of the EGFR protein kinase [PDB code 1M17]. Finally, toxicity risk and physicochemical characterization was performed for most of the compounds, revealing excellent properties as possible drugs, especially compounds 5c and 5h.