bluuebunny/crossref_metadata_2025_split huggingface dataset:
The importance of the IPC lead
Authors: Bridges Glenys 
Published: August 2023

Glenys Bridges details how the infection prevention and control (IPC) lead delivers safe and sustainable infection control. Aim This article aims to explore the essential requirements for effective infection prevention and show how these are fulfilled by infection control leads (IPC). It will identify the available resources and support available to maintain required Infection Prevention and Control standards and explain the importance of creating sustainable IPC through delegating and allocating roles and tasks. Objectives Develop the decon lead role to assure infector control outcomes. Specify the infection control leads role and job description. Define appropriate IPC training and development opportunities for the team.
Application of a fiber Bragg grating temperature sensing method based on support vector regression optimized by a genetic algorithm for the decreasing external ambient temperature case
Authors: Li Yingjie , Chen Tao , Si Jinhai , He Yingsong , Gao Bo , Hou Xun 
Published: August 2023

We studied the application of the fiber Bragg grating (FBG) temperature sensing method based on support vector regression optimized by a genetic algorithm (GA-SVR) for constant and decreasing external ambient temperature cases by simulation. The external ambient temperature could be retrieved from both the transient FBG wavelength and its corresponding change rate using GA-SVR, before the FBG temperature sensor reached the thermal equilibrium state with the external ambient temperature. FBG wavelengths and their corresponding change rates in the cases of FBG sensor temperatures higher and lower than the external ambient temperature were studied and used to construct the training data set. We found that there exist singularity points in the curves of the wavelength change rate when the FBG sensor temperature is higher than the external ambient temperature in some cases, which is different from the case where the FBG sensor temperature is lower than the external ambient temperature. Its application for sensing the constant and decreasing external ambient temperature in real time was demonstrated with an accuracy of 0.32°C in those two cases. It also indicates that for real applications of this temperature sensing method where the external ambient temperature varies randomly, the FBG sensor temperature changes rather than the external ambient temperature changes play the dominant role. What is more, the demodulation time was decreased to 0.002 s, which is approximately 0.05‱ of the time constant of the FBG temperature sensor. In other words, this method makes it possible to realize the external ambient temperature determination using a time smaller than the time constant of the FBG sensor. The high sensing accuracy and fast demodulation speed are crucial for future high-performance real-time FBG temperature sensing.
Global histone H2B degradation regulates insulin/IGF signaling‐mediated nutrient stress
Authors: Zhu Zhiwen , Li Dongdong , Jia Zeran , Zhang Wenhao , Chen Yuling , Zhao Ruixue , Zhang Yan‐Ping , Zhang Wen‐Hong , Deng Haiteng , Li Yinqing , Li Wei , Guang Shouhong , Ou Guangshuo 
Published: August 2023

AbstractEukaryotic organisms adapt to environmental fluctuations by altering their epigenomic landscapes and transcriptional programs. Nucleosomal histones carry vital epigenetic information and regulate gene expression, yet the mechanisms underlying chromatin‐bound histone exchange remain elusive. Here, we found that histone H2Bs are globally degraded in Caenorhabditis elegans during starvation. Our genetic screens identified mutations in ubiquitin and ubiquitin‐related enzymes that block H2B degradation in starved animals, identifying lysine 31 as the crucial residue for chromatin‐bound H2B ubiquitination and elimination. Retention of aberrant nucleosomal H2B increased the association of the FOXO transcription factor DAF‐16 with chromatin, generating an ectopic gene expression profile detrimental to animal viability when insulin/IGF signaling was reduced in well‐fed animals. Furthermore, we show that the ubiquitin‐proteasome system regulates chromosomal histone turnover in human cells. During larval development, C. elegans epidermal cells undergo H2B turnover after fusing with the epithelial syncytium. Thus, histone degradation may be a widespread mechanism governing dynamic changes of the epigenome.
Abnormal Uterine Bleeding in Adolescence Burdened with Severe Anemia: Clinical Case
Authors: Adamyan Leila V. , Sibirskaya Elena V. , Karachentsova Irina V. , Pivazyan Laura G. , Loshkareva Maria A. , Kirillova Yuliya A. , Murvatova Sabina K. , Murvatova Kamila K. 
Published: August 2023

Background. Abnormal uterine bleeding (AUB) in adolescence is one of the urgent problems of medicine nowadays. It is on the 2nd place among all the reasons for hospitalization in gynecological departments, and its incidence among girls during puberty is 50%. Clinical case description. This is the clinical case of 15-year-old girl admitted to the gynecology department with complaints on long-lasting bloody vaginal discharge, fatigue, dizziness. The diagnosis of «abnormal uterine bleeding in adolescence» burdened with iron deficiency anemia (IDA) was established according to laboratory and instrumental examinations. The patient underwent RBC-transfusion with specific buffy coat and was administered with hormonal hemostatic and anti-anemic therapy. Conclusion. We can conclude (with reference to this observation and the reviewed literature) not only that IDA is one of the most common complications of AUB in adolescence, but also what management should be used in such clinical cases.
Synthesis and properties of polymer electrolytes based on polyurethane elastomer and lithium salts
Authors: Fedorov Nikita , Ulihin Artem , Uvarov Nikolai 
Published: August 2023

Polymer solid electrolytes were obtained by swelling the polyurethane elastomer with solutions of lithium salts LiBF4 and LiClO4 in DMSO at different concentration of lithium salt. The swelling effect was found to decrease with the increase in the salt concentration, whereas, the ionic conductivity has a maximum of 6–8·10–4 S/cm at 5 wt.% lithium salt. The salt solutions incorporated into the polymer pores have melting points ranging from –10 to 2 °C and de-swelling takes place at low temperatures. The obtained polyurethane elastomer materials have a high conductivity and may be promising for use in flexible lithium polymer batteries.
Features of 12-lead electrocardiography in patients with critical aortic stenosis
Authors: Komlev A. E. , Blinova E. V. , Sakhnova T. A. , Saidova M. A. , Imaev T. E. 
Published: August 2023

Aim. To study the features of 12-lead electrocardiography (ECG) in patients with critical aortic stenosis referred for transcatheter aortic valve implantation.Material and methods. The study included 50 patients aged 76,6±6,5 years. The presence of intraventricular block, voltage criteria for left ventricular hypertrophy (LVH), fragmented QRS complex, and repolarization alternans were assessed on the ECG. The frontal QRS-T angle (fQRS-Ta) was estimated as the absolute value of the difference between the QRS axis and T wave axis.Results. Intraventricular blocks were present in 10 (20%) patients: 8 (36%) patients with pulmonary hypertension (PH), 2 (7%) patients without PH (p=0,028). Voltage criteria for LVH were present in 24 (60%) patients. In the presence of voltage criteria for LVH, the mean aortic valve gradient, the left ventricular mass index (LVMI) and the aortic valve area (AVA) were significantly greater. Fragmented QRS complex in the chest leads was more common in patients with past myocardial infarction, intraventricular block, with PH. Strain pattern was present in 9 (23%) patients, while nonspecific repolarization changes — in 27 (67%) patients. Patients with strain pattern had significantly higher mean pressure gradient and more often had PH. Correlations were found between fQRS-Ta and mean pressure gradient (r=0,39; p=0,005), AVA (r=-0,40; p=0,004), LVMI (r=0,30; p=0,03). The fQRS-Ta was significantly greater in the presence of PH — 128±48o, while without PH — 97±49o (p=0,03).Conclusion. In the studied group of patients with aortic stenosis, intraventricular block, fragmented QRS in the chest leads, strain pattern were more common in the presence of PH. In the presence of voltage criteria for LVH, LVMI, mean pressure gradient were significantly greater, while AVA — lower. The fQRS-Ta was significantly greater in the presence of intraventricular blocks, PH, as well as orrelated with mean pressure gradient and AVA.
Lipoprotein(a) in the diagnosis of cardiovascular risk. The values of lipoprotein (a) and apolipoprotein B in the adult population of Krasnoyarsk
Authors: Emelyanchik V. S. , Marilovtseva O. V. , Khomchenkov R. V. , Moiseeva A. M. , Chernova A. A. , Emelyanchik E. Yu. , Nikulina S. Yu. , Protopopov A. V. 
Published: August 2023

Aim.To study the prevalence of elevated concentrations of lipoprotein (a) (Lp(a)) and apolipoprotein B (apoB) associated with high cardiovascular risk in a random sample of the adult Krasnoyarsk population.Material and methods.Lp(a) and apoB concentrations were determined by the immunochemiluminescent method using an automated modular Cobas 8000 platform with biochemical module c702 (Roche Diagnostic, Switzerland). We examined 263 people aged 18 to 58 who agreed to expand the standard screening by Lp(a) and apoB determination.Results.We found that high and very high levels of Lp(a) are found in 12,9% of the examined, while in women the median value was significantly higher than in men (p=0,044). In addition, 11,4% of study participants had high apoB values, regardless of sex; 3,4% of the subjects had a combination of elevated levels of ap B and Lp(a).Conclusion.Screening for Lp(a) and apoB reveals dyslipidemia associated with high cardiovascular risk in 24,3% of working-age people
Rola regulatorów transkrypcji w kontroli ekspresji genów prokariotycznych na przykładzie modelowej bakterii Pseudomonas aeruginosa
Authors: Modrzejewska-Balcerek Magdalena , Bartosik Aneta Agnieszka 
Published: August 2023

Bakterie Pseudomonas aeruginosa spotykane są często w środowisku naturalnym jak woda czy gleba, ale zaliczane są także do oportunistycznych patogenów ludzi i zwierząt. Charakteryzuje je duża zdolność do przetrwania w bardzo różnych niszach ekologicznych. Adaptacja i umiejętność dostosowania do zmiennych warunków środowiska wynika z rozbudowanych sieci regulacyjnych tych bakterii i wykorzystania bogatego repertuaru kodowanych w genomie białek, ścieżek metabolicznych, mechanizmów obronnych i adaptacyjnych.
Regulatory transkrypcji są kluczowymi elementami regulacji ekspresji genów, które odpowiadają na sygnały z otoczenia, włączając lub wyłączając określone ścieżki. Kompleksowe badania regulatorów transkrypcji z wykorzystaniem metod transkryptomicznych, genomicznych, regulacyjnych dostarczają wiedzy na temat mechanizmów ich działania, regulowanych genów i procesów, umożliwiając poznanie i zrozumienie skomplikowanych często sieci regulacyjnych zawiadujących przeżyciem komórki w danym środowisku i warunkach.
Celem niniejszej pracy jest przedstawienie kluczowych zagadnień związanych z regulacją transkrypcji bakteryjnej na podstawie przeglądu danych literaturowych oraz zobrazowanych na przykładzie P. aeruginosa i charakterystyki mechanizmów regulacji ekspresji genów zaangażowanych między innymi w wirulencję tej bakterii.
PRIME EDITING - nowa metoda edycji genów
Authors: Blicharska Dalia , Szućko-Kociuba Izabela , Filip Ewa , Orłowska Anna , Skuza Lidia 
Published: August 2023

Metoda Prime Editing’u polega na wprowadzeniu oczekiwanych edycji w obrębie danego genomu przy zastosowaniu konstruktu zbudowanego z nikazy Cas9, pegRNA oraz odwrotnej transkryptazy, odpowiedzialnej za syntezę odcinka, który ma następnie zostać włączony do edytowanej nici. Technika ta bazuje na wcześniej odkrytej metodzie CRISPR/Cas9. Różni się od CRISPR/Cas9 brakiem generowania podwójnych pęknięć w obrębie helisy DNA co zawdzięcza swojej złożonej konstrukcji, w tym m.in. obecności dodatkowych elementów tj.: odwrotnej transkryptazy oraz matrycy zawartej w obrębie pegRNA. PE wykorzystuje się do wprowadzania modyfikacji w obrębie podwójnej helisy DNA.
Praca porusza głównie tematykę kreowania i udoskonalania oraz testowania nowoczesnej metody Prime Editing’u. Podano informacje na temat budowy oraz sposobu funkcjonowania systemu, a także opisano dotychczas przeprowadzone badania z zastosowaniem PE, wykonane w obrębie genomów komórek pochodzących z organizmów roślinnych, zwierzęcych oraz ludzkich. W pracy zawarto również informacje dotyczące potencjalnych korzyści oraz nadzieje związane z zastosowaniem tej innowacyjnej metody.
Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line
Authors: Mehrabi Maryam , Alemohammad Sara , Dashtebozorgi Reza , Tahmasebi Birgani Maryam , Hajjari Mohammad Reza , Mohammadi-Asl Javad 
Published: August 2023

Introduction: Breast cancer is one of the most common malignancies in women. Treatment with herbal drugs has been considered by researchers due to the lower side effects. Curcumin is a polyphenol extracted from turmeric with confirmed anti-cancer properties. Curcumin is water-insoluble with rapid metabolism. Drug delivery using nano-carriers is suggested to overcome such limitations. The aim of this study was based on evaluating the effect of Dendrosomes curcumin on expression level of HOTAIR long noncoding RNA in MCF-7 breast cancer cell line.
Methods: This study was an applied basic research. Firstly, curcumin was loaded in dendrosomes and the entry of dendrosomal curcumin into cells was studied using fluorescent microscopy. Cell death was investigated using MTT assay and apoptosis detection kit. The expression of HOTAIR gene was measured using real-time PCR. Increased expression of this gene was reported in a wide range of tumors. The data were analyzed using GraphPad Prism V9.5 statistical software, using NOVA one-way statistical analysis and Student t-test; the results were reported as mean ± standard deviation.
Results: Dendrosomes increased the solubility of curcumin. The effective inhibitory doses of dendrosomal curcumin after 24 and 48h treatment were 25 and 20 micromolar, respectively. The percent of cells undergoing early apoptosis were 22.97±0.03 and 56.22±0.05, respectively, which was statistically significant in comparison with non-treated control cells. Following 24 and 48h treatment with 20 micromolar of dendrosomal curcumin, the HOTAIR gene expression was significantly decreased (P=0.001).
Conclusion: These findings suggest that dendrosomal curcumin may promote breast tumor cells toward programmed cell death by reducing HOTAIR gene expression.
Conhecimento tradicional dos pescadores artesanais sobre a bioecologia do pirarucu (Arapaima spp.) na microrregião do Tapará, Santarém-Pará-Brasil
Authors: Lemos Tatiane Almeida , Porto Braga Tony Marcos , Lima da Silva Alanna Do Socorro 
Published: August 2023

Diante da importância do Pirarucu (Arapaima spp.) para as comunidades ribeirinhas da Amazônia, o objetivo deste estudo é o de analisar o conhecimento biológico sobre a espécie em áreas com e sem manejo pesqueiro, e como esse conhecimento influencia na captura da espécie na região. As informações foram coletadas por intermédio de formulários semiestruturados. As comunidades foram dispostas em dois grupos: a) comunidades que têm manejo do pirarucu (CCMP): Santa Maria e Tapará-miri; e b) comunidades que não têm manejo do pirarucu (CSMP): Barreira e Correio do Tapará. Os dados foram analisados por intermédio de estatística descritiva e cognição comparada. O estudo demonstrou que os pescadores artesanais, nas duas categorias de comunidades analisadas, apresentam vasto conhecimento sobre a ecologia do pirarucu e este, está em conformidade com a literatura cientifica.
Plano de gestão de logística sustentável para uma instituição de ensino superior: um plano de ações
Authors: Cordeiro Marcus Vinicius Cruz , Nadae Jeniffer De , Nascimento Diego Coelho do , Moura-Fé Marcelo Martins de , Ramos Ingrid Mazza Matos 
Published: August 2023

O trabalho analisa a viabilidade de um plano de ações destinado à implantação de um Plano de Gestão de Logística Sustentável para uma Instituição de Ensino Superior, localizada na cidade de Juazeiro do Norte-CE. Instituições de Ensino Superior, além da produção de conhecimento, podem servir como exemplos para a sociedade, na adoção de práticas de sustentabilidade, cumprindo o normativo legal e também sua função social. Para tanto, foi realizado um diagnóstico da situação atual da instituição, por meio de pesquisa exploratória, bibliográfica e estudo de caso, visando a verificação de práticas e ações já utilizadas pela instituição e observando-se as condições favoráveis à implementação da proposta aventada, a qual foi elaborada com a ferramenta 5W2H, através de 07 (sete) ações-chave, culminando com a implementação de um PLS sólido e eficaz a ser adotado pelo órgão público abordado.
Policy Environment Analysis in Indian Fertiliser Industry
Authors: Sharma Seema 
Published: August 2023

The objective of this paper is to analyse the impact of Government policy decisions in fertiliser industry.Â Indian Government has taken two strategic decisions in this industry viz., introduction of Retention Price Scheme in 1976 and the decontrol of phosphatic and potassic fertilisers in 1992. The two tier pricing implemented in the first decision has made a positive impact on the growth of this industry and the agricultural production.Â However the second decision to partially decontrol the phosphatic and potassic fertilisers and keep urea under control has led to a lopsided environment in this industry. This growing distortion in the nitrogenpous, phosphatic and potassic (NPK) usage in Indian agriculture is a threat to agricultural productivity growth. Therefore, there is a need to come out with sustainable policy which will not only improve the NPK balance in the economy but also improve the health of Indian fertiliser industry by ensuring profits to the producers. The fertilizer industry need tom be in line with rest of the economy hence a phased decontrol is thus considered is desirable so that the farmers interests can be protected.
The difference of Bishop score change and labor event between oral and vaginal misoprostol in pregnancy beyond 41 weeks
Authors: Maskasoni Maskasoni , Dewantiningrum Julian 
Published: August 2023

HIGHLIGHTS

Oral misoprostol is more effective than vaginal misoprostol in cervical ripening as a part of induction of labor.
Oral misoprostol is as safe as vaginal misoprostol.

ABSTRACT
Objective: To compare Bishop score changes and labor event between oral and vaginal misoprostol in pregnancy beyond 41 weeks.
Materials and Methods: A total of 52 pregnant women with more than 41 weeks of gestation, had a Bishop score less than 5, and were undergoing induction labor were randomly divided into two groups: oral and vaginal misoprostol. In the oral misoprostol group, participants were given 25 mg of misoprostol in a solution with a concentration of 1 ug/ml every 2 hours. In the vaginal misoprostol group, a 25 mg misoprostol tablet was inserted into the posterior fornix every 6 hours. The two groups were compared in terms of Bishop score during the first 6 hours, changes in Bishop score, labor at term events, neonatal outcomes, complications, and side effects after the administration of misoprostol.
Results: The oral group showed significantly higher changes in Bishop score compared to the vaginal group (5.5 vs 3.6; p=0.0001). The median interval times for induction of labor at term, induction at stage II, and induction at birth were found to be shorter in the oral misoprostol group compared to the vaginal group (7.3 hours vs 10.6 hours, 14.0 hours vs 16.8 hours, and 14.6 hours vs 17.6 hours; p=0.002, 0.003, 0.002). Labor at term occurred much more frequently in the oral group (53.8% vs 15.4%). Additionally, the oral misoprostol group had a 3.5 times higher likelihood of experiencing labor at term within the first 6 hours after the initial administration compared to the vaginal group (OR 3.5, 95% CI 1.33-9.23).
Conclusion: Oral administration of misoprostol for cervical ripening has been demonstrated to be more effective than vaginal administration, greater bishop score changes while maintaining an equivalent level of safety.
CRIAÇÃO E CONSOLIDAÇÃO DA LINHA DE PESQUISA ENSINO DE GEOGRAFIA NO PROGRAMA DE PÓS-GRADUAÇÃO EM GEOGRAFIA DA UFT
Authors: Pereira Carolina Machado Rocha Busch 
Published: August 2023

Em razão da comemoração dos 10 anos do Programa de Pós-graduação em Geografia (PPGG) da Universidade Federal do Tocantins este artigo tem como objetivo apresentar o percurso de criação e os desdobramentos que contribuíram para a consolidação da área de pesquisa em Ensino de Geografia no PPGG. A partir de um conjunto de fontes históricas, da produção acadêmica e de registros de pesquisas busca-se explicitar as ações que resultaram na constituição da linha de pesquisa em Ensino de Geografia no PPGG da UFT. O Programa de Pós-graduação em Geografia da Universidade Federal do Tocantins foi implantado em 2011 com duas linhas de pesquisa: "Análise e Gestão Geoambiental" e "Estudos Geoterritoriais" e permaneceu até 2016 com esta configuração. A alteração das linhas de pesquisa no PPGG ocorreu em 2016 mediante a demanda que crescia ano após ano por orientações na área de ensino de Geografia. Em 2017 o PPGG incluiu a linha de pesquisa “Ensino de Geografia” no processo seletivo procurando destacar a Geografia na dimensão do ensino com espaço para a reflexão, pesquisa e debate sobre a Geografia no contexto da educação escolar, a formação de professores, a relação entre Geografia e educação formal e não-formal, a relação ensino aprendizagem na Geografia, cultura-arte-memória-imaginário no ensino da Geografia, tecnologias e práticas pedagógicas, ensino de Geografia e Educação Ambiental, e as categorias geográficas na relação do ensino aprendizagem. Durante esses 6 anos de formação de mestres na área de ensino de Geografia o PPGG contabiliza 15 dissertações defendidas (até abril de 2023) e 7 em andamento.