bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Feasibility of using Gag proteins to reawaken dormant HIV infection based on bioinformatics analysis
Authors: Chen S. 
Published: August 2023

Abstract

Recent studies revealing varied responses of infected cells to LRAs underscore the limited effectiveness of these agents and emphasize the wide array of determinants contributing to the heterogeneity of reservoirs, including virus genetic background, cell model, cell type, silencing mechanisms, tissue reservoirs, integration sites, patient, and gender specific factors. The enhancer region of the HIV-1 LTR contains two adjacent NF-κB binding sites that play a central role in mediating inducible HIV-1 gene expression. Beyond the involvement of various transcription factors, such as NF-κB, epigenetic constraints also play a pivotal role in suppressing the initiation of latent HIV transcription. Consequently, even latent viruses containing functional NF-κB sites remain unresponsive to drugs that activate NF-κB. Thus, it is evident that the activation of NF-κB alone does not suffice to trigger latent HIV, contradicting the central hypothesis of this study. The author used bioinformatics methods to analyze the viral proteins and their primer binding sites. The results show that the amino acid sequence ARG of Gag proteins of HTLV-1, HTLV-2, STLV-1 and STLV-2 match their primer binding site GGGGGCTCG in the 3'-to-5' direction and that the amino acid sequence SPR of Gag proteins of HIV-1, HIV-2, SIV and FIV match their primer binding site GGCGCCCGA in the 3'-to-5' direction. Related studies have shown that the genomic Gag/Gag-Pol complex recruits the LysRS/tRNA complex. The selective packaging of the tRNA primer requires HIV-1 Gag and Gag-Pol, and an interaction between LysRS and Gag is observed in vitro. In HIV-1, Gag/LysRS interaction depends on Gag sequences within the CTD of CA around amino acids 283-363 and motif 1 of LysRS around amino acids 208-259. It should be noted that the amino acid sequence SPR of the Gag protein is located at amino acids 148-150 within the NTD of CA, specifically at the NTD-NTD interface 1. Although this research is purely bioinformatics analysis, the relevant studies have demonstrated that Gag proteins match the HIV-1 primer binding site and possess the potential to directly activate dormant retroviruses.
EFR3A: a new raft domain organizing protein?
Authors: Trybus Magdalena , Hryniewicz-Jankowska Anita , Wójtowicz Karolina , Trombik Tomasz , Czogalla Aleksander , Sikorski Aleksander F. 
Published: August 2023

Abstract

Background: Membrane rafts play a crucial role in the regulation of many important biological processes. Our previous data suggest that specific interactions of flotillins with MPP1 are responsible for membrane raft domain organization and regulation in erythroid cells. Interaction of the flotillin-based protein network with specific membrane components underlies the mechanism of raft-domain formation and regulation, including in cells with low expression of MPP1.
Methods: We sought to identify other flotillin partners via the immobilized recombinant flotillin-2-based affinity approach and mass spectrometry technique. The results were further confirmed via immunoblotting and via co-immunoprecipitation. In order to study the effect of the candidate protein on the physicochemical properties of the plasma membrane, the gene was knocked down via siRNA, and fluorescence lifetime imaging microscopy and spot-variation fluorescence correlation spectroscopy was employed.
Results: Thereby EFR3A was identified as a candidate protein that interacts with flotillin-2. Moreover, this newly discovered interaction was demonstrated via overlay assay using recombinant EFR3A and flotillin-2. EFR3A is a stable component of the detergent-resistant membrane fraction of HeLa cells, and its presence was sensitive to the removal of cholesterol. While silencing the EFR3A gene, we observed decreased order of the plasma membrane of living cells or giant plasma membrane vesicles derived from knocked down cells and altered mobility of the raft probe, as indicated via fluorescence lifetime imaging microscopy and spot-variation fluorescence correlation spectroscopy. Moreover, silencing of EFR3A expression was found to disturb epidermal growth factor receptor and phospholipase C gamma phosphorylation and affect epidermal growth factor-dependent cytosolic Ca2+ concentration.
Conclusions: Altogether, our results suggest hitherto unreported flotillin-2-EFR3A interaction, which might be responsible for membrane raft organization and regulation. This implies participation of this interaction in the regulation of multiple cellular processes, including those connected with cell signaling which points to the possible role in human health, in particular human cancer biology.
Outer inverses in semigroups belonging to the prescribed Green’s equivalence classes
Authors: Ćirić Miroslav , Ignjatović Jelena , Stanimirović Predrag 
Published: August 2023

Abstract

We provide existence criteria and characterizations for outer inverses in a semigroup belonging to the prescribed Green’s ℛ-, ℒ- and ℋ-classes. These results generalize the well-known problem of finding outer inverses of a matrix over a field with the prescribed range or/and null space. Outer inverses belonging to the prescribed Green’s ℛ- and ℒ-classes also represent extensions of Drazin’s (b, c)-inverses and Mary’s inverses along an element. We provide an overview of other such extensions that have emerged recently and compare them with the extensions introduced in this paper.

Mathematics Subject Classification (2010). 20M99, 15A09, 15A24.
Repurposing Coffee Husk By-Products (Cascara) for the Production of Kombucha Beverage
Authors: Van Thach Phan , Phan Quang Khai , Pham Gia Bao , Quang Hoa Pham , Do Anh Duy 
Published: August 2023

Abstract
Kombucha is a well-known fermented beverage that offers various nutritional and health benefits to humans. Various substrates have been considered for kombucha fermentation, including herbs, fruits, flowers, and vegetables, to enhance flavors, odor, and nutritional value. In this study we examined the potential of cascara or coffee husk, as a substrate for kombucha production. Our results indicated that a combination of cascara and a sucrose concentration of 10g/L was appropriate for the fermentation of kombucha. The cascara kombucha achieved a low pH of 2.69 after a 14-day of fermentation, which corresponds to microbiological safety requirements. Additionally, fermentation increased polyphenols, flavonoids, and total acids content, resulting in antioxidant, antimicrobial, and prebiotic activities that promote a healthy gut microbiota. Furthermore, 14 days of fermentation has been demonstrated for achieving a balanced sensory profile, including sourness, sweetness, and odor, as well as enhancing the health-promoting nutrient accumulation. These findings emphasize the potential health benefits of cascara kombucha, making it an appealing beverage for consumers at the same time, while also repurposing the coffee industry by-products, contributing to sustainable and environmentally friendly development.
Does Workplace Bullying Responsible for Low Job Performance? A Twofold SEM-ANN Approach
Authors: Alam Mohammad Nurul , Rehman Imdadullah Hidayat ur , Dhall Rosy , Ali Jamshid , Gazi Md. Abu Issa 
Published: August 2023

Abstract
Purpose- Workplace bullying is one of the phenomenal supervisory tool using in the garment industry of Bangladesh. The productivity of this industry workers are the lowest as compared with its competitors such as China, Vietnum, India, Thailand, Pakistan, Srilanka, Combodia etc. Therefore, the primary purpose of this study was to examine whether the workplace bullying responsible for low job performance in the garment industry of Bangladesh.
Methodology- The study is based on quantitative data, collected through a survey questionnaire adapted from previous studies. The target population was the workers from the ready-made garment industry in Bangladesh. Moreover, simple random sampling technique was used to collect 384 samples. Additionally, Smart-PLS 3.3.9 and SPSS (v23) were employed for preliminary data examination and to examine the proposed hypotheses. A two-fold SEM-ANN approach was used to validate the proposed model of the study.
Findings- The findings of the study revealed that workplace bullying negatively affect on employees’ job performance. Psychological stress and burnout play a negative mediating effect between workplace bullying and job performance. Additionally, perceived organizational support plays a significant moderating role among workplace bulling and it’s outcome variables such as job performance, psychological stress, burnout, and organizational citizenship behaviour. However, organizational citizenship behaviour does not play any significant mediating role in the relationship between workplace bulling and job performance.
Implication/Originality- The study has been unique in nature, as it focuses on the workers in in the developing country context like Bangladesh where the employees encounter tremendously with bulling from supervisor and higher management as their regular practice. The outcomes of the consequences are psycological stress, burnout, organizational citizenship behaviours, and low job performance enourmusly which has been highlighted in the current study.
Can Machine Learning Catch Economic Recessions Using Economic and Market Sentiments?
Authors: Tehranian Kian 
Published: August 2023

Abstract
Quantitative models are an important decision-making factor for policy makers and
investors. Predicting an economic recession with high accuracy and reliability would be
very beneficial for the society. This paper assesses machine learning technics to predict
economic recessions in United States using market sentiment and economic indicators
(seventy-five explanatory variables) from Jan 1986 – June 2022 on a monthly basis
frequency. In order to solve the issue of missing time-series data points, Autoregressive
Integrated Moving Average (ARIMA) method used to backcast explanatory variables.
Analysis started with reduction in high dimensional dataset to only most important
characters using Boruta algorithm, correlation matrix and solving multicollinearity issue.
Afterwards, built various cross-validated models, both probability regression methods
and machine learning technics, to predict recession binary outcome. The methods
considered are Probit, Logit, Elastic Net, Random Forest, Gradient Boosting, and Neural
Network. Lastly, discussed different model’s performance based on confusion matrix,
accuracy and F1score with potential reasons for their weakness and robustness.
The Practice of Livelihood Diversification in Rural Bale Zone: The Case of Sinana District, Oromia, Ethiopia
Authors: Girma Berhanu , Kerorsa Gemechu 
Published: August 2023

Abstract

Rural households diversify their activities into non-farm and off-farm activities. This study, mainly strives to Analyze the practice of livelihood diversification in rural Bale Zone; the case of Sinana District, Oromia, Ethiopia. Primary data was gathered using structured survey questionnaires that carried out by well-trained enumerators and supervisors. Using multistage sampling design a total sample of 335 households were selected. Double hurdle model was used to identify factors affecting decision and extent of participation in livelihood diversification. The results suggested that education of house hold head, household size, distance to nearest market, oxen owned, access to credit, access to training and total annual income were found to be significant in influencing household’s livelihood diversification decision. Moreover, age of household head, sex of household head, education of household head, community based organization membership, access to mass media, access to training, landholding size, and climate shock were found to be significant in influencing extent livelihood diversification. Finally the study has concluded that the households in the study area are likely to have a diversified livelihood when they have higher annual income, educational level, proximity to nearest market, access to training and access to credit facilities. Moreover once households decide to participate in livelihood diversification activities, the extent of livelihood diversification gets boosted when there is higher educational level, access to mass media, access to training and community based organization membership. Therefore, the government is recommended to build human capital through education, skills and training opportunities to motivate rural households to engage in livelihood diversification in order to ensure income security, increased well-being, reduced vulnerability and sustainable use of natural resources.
Media critiques on the Merauke Integrated Food and Energy state (MIFEE) program: A critical discourse perspective
Authors: Wahyudin Ahmad , Dwi Harjanto Tofan 
Published: August 2023

The study is a critical discourse analysis (CDA) on the media critiques on the implementation of the Merauke Integrated Food and Energy Estate (MIFEE) program. The study is aimed at describing the socio-cultural practices of the news items in the mass media about the MIFEE program. The sociocultural practice analysis is a CDA in the macro level, which is related to contexts outside the texts. Contexts, in this case, include situational contexts, i.e. related to a specific society, culture, or polity. The study is qualitative research; data sources are news items about critiques to the MIFEE; data collection is done by the listening and note taking technique; and data analyses are done by the pragmatic equivalence method. The study is backed by content and semantic data validities. Reliability of the study is assessed by intra- and inter-rater reliability techniques. Findings show that criticques to the MIFEE program can be described under five categories, namely: 1) critiques on the disruption of the forest ecosystem, 2) critiques on the system of the local food, 3) critiques on the violation of human rights, 4) critiques on land forfeiture, and 5) critiques on the failure of the MIFFE program. Keywords: MIFEE, critical discourse analysis (CDA), food resilience and self-provision, indigeneous culture
Gender ideology and power relations in Sahebjam’s Stoning of Soraya M: A feminist critical discourse analysis
Authors: Siboro Meliana 
Published: August 2023

The context of the study is the ongoing protests and demonstrations by women in many places in Iran. The aim of this study is to examine gender ideology and power in Sahebjam’s Stoning of Soraya M. Qualitative descriptive method is used to investigate the relationship between gender ideology and power relations and how it creates power imbalance between men and women in Iranian society. The primary data source for this research is a novel, Stoning of Soraya M by Freidoune Sahebjam, which was first published in September 12, 1990. Data are analyzed through the lens of Critical Discourse Analysis approach, more specifically, Lazar’s Feminist Critical Discourse Analysis (2007). The findings of the study suggest that the country's laws, including the Constitution, the Civil Code, and the Criminal Code create a complicated legal environment for women. While the Constitution guarantees equal civil rights for men and women, religious law superseds other laws and is frequently abused by individuals to commit crimes against women. This study reveals how gender ideology and power relations regarding women reflected through discourse can demonstrate how women are treated in different parts of the world.Keywords: Gender Ideology, Power, Novel, Feminist Critical Discourse Analysis
Patient-Caregiver Portal System in Palliative Oncology: Assessment of Usability and Perceived Benefit
Authors: Longacre Margaret L , Chwistek Marcin , Keleher Cynthia , Siemon Mark , Egleston Brian L , Collins Molly , Fang Carolyn Y 
Published: August 2023

Background
The engagement of family caregivers in oncology is not universal or systematic.

Objective
We implemented a process intervention (ie, patient-caregiver portal system) with an existing patient portal system to (1) allow a patient to specify their caregiver and communication preferences with that caregiver, (2) connect the caregiver to a unique caregiver-specific portal page to indicate their needs, and (3) provide an electronic notification of the dyad’s responses to the care team to inform clinicians and connect the caregiver to resources as needed.

Methods
We assessed usability and satisfaction with this patient-caregiver portal system among patients with cancer receiving palliative care, their caregivers, and clinicians.

Results
Of 31 consented patient-caregiver dyads, 20 patients and 19 caregivers logged in. Further, 60% (n=12) of patients indicated a preference to communicate equally or together with their caregiver. Caregivers reported high emotional (n=9, 47.3%), financial (n=6, 31.6%), and physical (n=6, 31.6%) caregiving-related strain. The care team received all patient-caregiver responses electronically. Most patients (86.6%, 13/15 who completed the user experience interview) and caregivers (94%, 16/17 who completed the user experience interview) were satisfied with the system, while, of the 6 participating clinicians, 66.7% agreed “quite a bit” (n=1, 16.7%) or “very much” (n=3, 50%) that the system allowed them to provide better care.

Conclusions
Our findings demonstrate system usability, including a systematic way to identify caregiver needs and share with the care team in a way that is acceptable to patients and caregivers and perceived by clinicians to benefit clinical care. Integration of a patient-caregiver portal system may be an effective approach for systematically engaging caregivers. These findings highlight the need for additional research among caregivers of patients with less advanced cancer or with different illnesses.
Impact of an Electronic Medical Record–Connected Questionnaire on Efficient Nursing Documentation: Usability and Efficacy Study
Authors: Kodama Kana , Konishi Shozo , Manabe Shirou , Okada Katsuki , Yamaguchi Junji , Wada Shoya , Sugimoto Kento , Itoh Sakiko , Takahashi Daiyo , Kawasaki Ryo , Matsumura Yasushi , Takeda Toshihiro 
Published: August 2023

Background
Documentation tasks comprise a large percentage of nurses’ workloads. Nursing records were partially based on a report from the patient. However, it is not a verbatim transcription of the patient's complaints but a type of medical record. Therefore, to reduce the time spent on nursing documentation, it is necessary to assist in the appropriate conversion or citation of patient reports to professional records. However, few studies have been conducted on systems for capturing patient reports in electronic medical records. In addition, there have been no reports on whether such a system reduces the time spent on nursing documentation.

Objective
This study aims to develop a patient self-reporting system that appropriately converts data to nursing records and evaluate its effect on reducing the documenting burden for nurses.

Methods
An electronic medical record–connected questionnaire and a preadmission nursing questionnaire were administered. The questionnaire responses entered by the patients were quoted in the patient profile for inpatient assessment in the nursing system. To clarify its efficacy, this study examined whether the use of the electronic questionnaire system saved the nurses’ time entering the patient profile admitted between August and December 2022. It also surveyed the usability of the electronic questionnaire between April and December 2022.

Results
A total of 3111 (78%) patients reported that they answered the electronic medical questionnaire by themselves. Of them, 2715 (88%) felt it was easy to use and 2604 (85%) were willing to use it again. The electronic questionnaire was used in 1326 of 2425 admission cases (use group). The input time for the patient profile was significantly shorter in the use group than in the no-use group (P<.001). Stratified analyses showed that in the internal medicine wards and in patients with dependent activities of daily living, nurses took 13%-18% (1.3 to 2 minutes) less time to enter patient profiles within the use group (both P<.001), even though there was no difference in the amount of information. By contrast, in the surgical wards and in the patients with independent activities of daily living, there was no difference in the time to entry (P=.50 and P=.20, respectively), but there was a greater amount of information in the use group.

Conclusions
The study developed and implemented a system in which self-reported patient data were captured in the hospital information network and quoted in the nursing system. This system contributes to improving the efficiency of nurses’ task recordings.
Assessment on the Pattern of Drug Information Queries in a Tertiary Care Hospital, Chennai
Authors: Singh S. Daniel Sundar , Shailaja K , Bharathi Priya. K , Leena Muppa , Dhivya K , Jeevitha S , Priyadharshini V 
Published: August 2023

The drug information services help to assist healthcare workers to address patient-specific drug-related needs and promote rational drug use. The objective of the study is to assess the pattern of drug information queries in a tertiary care hospital from the Physician, Nurses, Pharmacists, Students, and other healthcare professionals. In one year, the drug information queries have been received, documented and retrospectively analyzed for various parameters including the professional status of the requestor, mode of receipt and reply, type and purpose of query, and reference details. Out of 588 queries received, the majority were answered by Physicians (n=468; 79.59%), followed by Pharmacists (n=55; 9.35%), Nurses (n=15; 2.55%), also Technicians (n=9; 1.53%), Students (n=21; 3.58%), Patients (n=3; 0.5%) and other health care professionals (n=17; 2.90%). The secondary resources (Micromedex/Medscape) were used majorly, followed by Textbooks (n=117; 19.89%), Internet (n=103; 17.51%), Journals (n=23; 3.9%) and others (n=5; 0.85%). The most common drug-related query was Pharmacological drug profile (n=149; 25.34%) and including Product identification (n=132; 22.44%), Product Information (n=84; 14.28%), Adverse Drug Reactions (n=74; 12.58%), Therapeutic uses (n=55; 9.35%) and others (n=94; 15.98%). Drug Information Services has been developed to promote rational prescribing patterns among prescribers, reduce medication errors and provide better clinical outcomes.
Keywords: Drug Information Centre (DIC), Query, Drug Information Services, Patient care, Tertiary Care Hospital, Clinical Pharmacist.