bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells
Authors: Wang Juan-Juan , Chen Ding-Xiong , Zhang Yu , Xu Xin , Cai Yan , Wei Wen-Qiang , Hao Jia-Jie , Wang Ming-Rong 
Published: August 2023

Abstract
Background
The mechanisms underlying the occurrence and development of esophageal squamous cell carcinoma (ESCC) remains to be elucidated. The present study aims to investigate the roles and implications of IGF2BP1 overexpression in ESCC.

Methods
IGF2BP1 protein expression in ESCC samples was assessed by immunohistochemistry (IHC), and the mRNA abundance of IGF2BP1 and INHBA was analyzed with TCGA datasets and by RNA in situ hybridization (RISH). The methylation level of the IGF2BP1 promoter region was detected by methylation-specific PCR (MSP-PCR). Cell viability, migration, invasion and in vivo metastasis assays were performed to explore the roles of IGF2BP1 overexpression in ESCC. RNA immunoprecipitation sequencing (RIP-seq) and mass spectrometry were applied to identify the target RNAs and interacting proteins of IGF2BP1, respectively. RIP-PCR, RNA pulldown, immunofluorescence (IF), gene-specific m6A PCR and RNA stability assays were used to uncover the molecular mechanisms underlying the malignant phenotypes of ESCC cells caused by IGF2BP1 dysregulation. BTYNB, a small molecular inhibitor of IGF2BP1, was evaluated for its inhibitory effect on the malignant phenotypes of ESCC cells.

Results
IGF2BP1 overexpression was detected in ESCC tissues and associated with the depth of tumor invasion. In addition, IGF2BP1 mRNA expression in ESCC cells was negatively correlated with the level of its promoter methylation. Knockdown of IGF2BP1 inhibited ESCC cell invasion and migration as well as tumor metastasis. Mechanistically, we observed that IGF2BP1 bound and stabilized INHBA mRNA and then resulted in higher protein expression of INHBA, leading to the activation of Smad2/3 signaling, thus promoting malignant phenotypes. The mRNA level of INHBA was upregulated in ESCC tissues as well. Furthermore, IGF2BP1 interacted with G3BP stress granule assembly factor 1 (G3BP1). Knockdown of G3BP1 also down-regulated the INHBA-Smad2/3 signaling. BTYNB abolished this activated signaling and significantly attenuated the malignant phenotypes of ESCC cells.

Conclusions
Elevated expression of IGF2BP1 is a frequent event in ESCC tissues and might be a candidate biomarker for the disease. IGF2BP1 overexpression promotes the invasion and migration of ESCC cells by activating the INHBA-Smad2/3 pathway, providing a potential therapeutic target for ESCC patients with high expression of IGF2BP1.
Heritability and recursive influence of host genetics on the rumen microbiota drive body weight variance in male Hu sheep lambs
Authors: Wang Weimin , Zhang Yukun , Zhang Xiaoxue , Li Chong , Yuan Lvfeng , Zhang Deyin , Zhao Yuan , Li Xiaolong , Cheng Jiangbo , Lin Changchun , Zhao Liming , Wang Jianghui , Xu Dan , Yue Xiangpeng , Li Wanhong , Wen Xiuxiu , Jiang Zhihua , Ding Xuezhi , Salekdeh Ghasem Hosseini , Li Fadi 
Published: August 2023

Abstract
Background
Heritable rumen microbiota is an important modulator of ruminant growth performance. However, no information exists to date on host genetics-rumen microbiota interactions and their association with phenotype in sheep. To solve this, we curated and analyzed whole-genome resequencing genotypes, 16S rumen-microbiota data, and longitudinal body weight (BW) phenotypes from 1150 sheep.

Results
A variance component model indicated significant heritability of rumen microbial community diversity. Genome-wide association studies (GWAS) using microbial features as traits identified 411 loci-taxon significant associations (P < 10−8). We found a heritability of 39% for 180-day-old BW, while also the rumen microbiota likely played a significant role, explaining that 20% of the phenotypic variation. Microbiota-wide association studies (MWAS) and GWAS identified four marker genera (Bonferroni corrected P < 0.05) and five novel genetic variants (P < 10−8) that were significantly associated with BW. Integrative analysis identified the mediating role of marker genera in genotype influencing phenotype and unravelled that the same genetic markers have direct and indirect effects on sheep weight.

Conclusions
This study reveals a reciprocal interplay among host genetic variations, the rumen microbiota and the body weight traits of sheep. The information obtained provide insights into the diverse microbiota characteristics of rumen and may help in designing precision microbiota management strategies for controlling and manipulating sheep rumen microbiota to increase productivity.
A genome-wide cross-trait analysis identifies genomic correlation, pleiotropic loci, and causal relationship between sex hormone-binding globulin and rheumatoid arthritis
Authors: Jiang Yuan , Liu Qianwen , Alfredsson Lars , Klareskog Lars , Kockum Ingrid , Jiang Xia 
Published: August 2023

Abstract
Background
Our study aims to investigate an intrinsic link underlying sex hormone-binding globulin (SHBG) and rheumatoid arthritis (RA), which remains inconclusive in observational settings.

Methods
Summary statistics were collected from the largest GWAS(s) on SHBG adjusted for BMI (SHBGadjBMI; Noverall = 368,929; Nmen = 180,094; Nwomen = 188,908), crude SHBG (Noverall = 370,125; Nmen = 180,726; Nwomen = 189,473), and RA (Ncase = 22,350; Ncontrol = 74,823). A genome-wide cross-trait design was performed to quantify global and local genetic correlation, identify pleiotropic loci, and infer a causal relationship.

Results
Among the overall population, a significant global genetic correlation was observed for SHBGadjBMI and RA ($$r_{{\text{g}}}$$

r
g

= 0.11, P = 1.0 × 10−4) which was further supported by local signal (1q25.2). A total of 18 independent pleiotropic SNPs were identified, of which three were highly likely causal variants and four were found to have effects on both traits through gene expression mediation. A putative causal association of SHBGadjBMI on RA was demonstrated (OR = 1.20, 95% CI = 1.01–1.43) without evidence of reverse causality (OR = 0.999, 95% CI = 0.997–1.000). Sex-specific analyses revealed distinct shared genetic regions (men: 1q32.1-q32.2 and 5p13.1; women: 1q25.2 and 22q11.21-q11.22) and diverse pleiotropic SNPs (16 in men and 18 in women, nearly half were sex-specific) underlying SHBGadjBMI and RA, demonstrating biological disparities between sexes. Replacing SHBGadjBMI with crude SHBG, a largely similar yet less significant pattern of results was observed.

Conclusion
Our cross-trait analysis suggests an intrinsic, as well as a sex-specific, link underlying SHBG and RA, providing novel insights into disease etiology.
Non-invasive analysis of heritage textiles with MA-XRF mapping—exploring the possibilities. The study of Bishop Jacques de Vitry's mitres and fragile medieval reliquary purses from Namur (Belgium)
Authors: Vanden Berghe Ina , Van Bos Marina , Vandorpe Maaike , Coudray Alexia 
Published: August 2023

Abstract
This manuscript explores the potential of macro-X-ray fluorescence (MA-XRF) for the non-invasive analysis of heritage textiles. XRF, especially with the portable instruments, is a well-known technique for the non-destructive examination of various cultural heritage objects. It allows analysis of elemental composition based on single-point measurements. However, large, or complex textiles require numerous analysis points to identify the materials used and correctly interpret the spectra. MA-XRF takes this type of research to the next level, as it is possible to visualise the element distribution over an entire mapped area. In this paper, we discuss the application of this technique to the study of complex and multi-layered textile objects from exceptional Belgian heritage collections, including two mitres attributed to Bishop Jacques de Vitry, dating between twelfth and thirteenth century, and two of the seven extremely fine medieval reliquary purses from Namur. These are very fragile, richly decorated textile objects whose current state of preservation is a major impediment to sampling. MA-XRF mapping was applied for the identification of the elements of different materials in a non-invasive manner, including metal threads, ink, dyes, and various materials used in illuminations. In addition to material identification, stratigraphic information was obtained from the visualisation of element distributions, and hidden structural details were discovered. MA-XRF was also tested on some areas with more relief, such as the embroidery and braid made with metal threads, and the undulated multi-layered structure of the parchment mitre. Even though the analyses here could not be carried out in optimal conditions, these locations could also be analysed, albeit at a lower resolution. Finally, the technique proved very effective as a tool for screening, allowing samples to be taken at a more informative and representative location and minimising sampling.
Retinal photograph-based deep learning system for detection of hyperthyroidism: a multicenter, diagnostic study
Authors: Dong Li , Ju Lie , Hui Shiqi , Luo Lihua , Jiang Xue , Nie Zihan , Zhang Ruiheng , Zhou Wenda , Li Heyan , Jonas Jost B. , Wang Xin , Zhao Xin , He Chao , Chen Yuzhong , Wang Zhaohui , Gao Jianxiong , Ge Zongyuan , Wei Wenbin , Li Dongmei 
Published: August 2023

Abstract
Background
Screening for hyperthyroidism using gold-standard diagnostic criteria in the general population is not cost-effective, leading to a relatively high rate of undiagnosed and untreated patients. This study aimed to establish a deep learning-based system to detect hyperthyroidism based on retinal photographs.

Methods
The multicenter, observational study included retinal photographs taken from participants in two hospitals and 24 health care centers throughout China. We first trained two models to identify hyperthyroidism: in model #1, the non-hyperthyroidism individuals were randomly selected, while in model #2, the non-hyperthyroidism group was matched for age and gender with the hyperthyroidism group. After internal validation, we selected the better model for further evaluation using external validation datasets.

Results
The study included 22,940 retinal photographs of 11,409 participants for the model development, and 3862 retinal photographs (1870 participants) which were obtained from two hospitals and four medical centers as the external validation datasets. Model #1 achieved a higher area under the receiver operator curve (AUC) than model #2 (0.907, 95% CI: 0.894–0.918 versus 0.850, 95% CI: 0.832–0.866) in the internal validation so that model #1 was used for further evaluation. In external datasets, model #1 reached AUCs ranging from 0.816 (95% CI 0.789–0.846) to 0.849 (95% CI 0.824–0.874) and achieved accuracies between 0.735 (95% CI 0.700–0.773) and 0.796 (95% CI 0.765–0.824). Heatmaps showed a focus of the DL-algorism on large fundus vessels and the optic nerve head.

Conclusions
Retinal fundus photographs may serve for DL systems for a cost-effective and non-invasive method to detect hyperthyroidism.
Anesthetic management of scapular Y-osteotomy using a combination of suprascapular nerve block and erector spinae plane block for Sprengel deformity associated with Klippel-Feil syndrome: a case report
Authors: Okada Mizuho , Tanaka Nobuhiro , Suzuka Takanori , Kadoya Yuma , Saisu Takashi , Kawaguchi Masahiko 
Published: August 2023

Abstract
Background
Klippel-Feil syndrome (KFS) occurs in 1/40,000 individuals and is characterized by cervical fusion. Thirty percent of patients with KFS present with Sprengel deformity, leading to orthopedic problems and limited shoulder abduction. No reports exist regarding anesthetic procedures for pediatric scapular osteotomies.

Case presentation
We report a case of a 4-year-and-7-month-old boy (95.6 cm, 14.7 kg) who underwent left scapular osteotomy. At the age of 8 months, he also underwent a right lower lobectomy due to a congenital pulmonary airway malformation. We decided to use a combination of suprascapular nerve block (SSNB), erector spinae plane block (ESPB), and general anesthesia. He received regular acetaminophen administration and fentanyl 5–10 μg/hour intravenously until 20 h postoperatively and remained on ≤ 2/10 in the Wong-Baker Face Scale (0: no hurt; 10: hurts worst).

Conclusion
The combination of SSNB and ESPB could be an option for perioperative analgesia for scapular osteotomies.
Tracking demands for seeking psychological help before and during the COVID-19 pandemic: a quanti-qualitative study
Authors: Coutinho Bruna M. C. , Anunciação Luis F. C. , Landeira-Fernandez Jesus , Krahe Thomas E. 
Published: August 2023

AbstractThe COVID-19 pandemic has placed unprecedented burdens on individuals and communities around the world. The isolation, fear, and uncertainty caused by the virus has led to increased rates of anxiety, depression, and other mental health issues. The pandemic has also had a disproportionate impact on individuals and communities with low income and socioeconomic status.ObjectiveTo shed light on the consequences of the pandemic on individuals from minorities and low-income areas, we investigate the main reasons that led patients who were referred to a social clinic of a private university in Rio de Janeiro to seek psychological treatment before (2019) and during the pandemic (2020 and 2021).MethodsWe conducted a quanti-qualitative study with a lexical analysis that evaluated 549 complaint forms of patients seeking treatment in these two distinct periods. Our analyses included descending hierarchical analysis (DHA) and correspondence factor analysis (CFA).ResultsFamily dynamics and communication factors play a dominant role in the reason for seeking therapy and psychological treatment. Additionally, our study suggested an increase in anxiety and panic attacks among other mental health issues associated with grief and losses during the pandemic years.ConclusionBased on these analyses, we can begin to identify a few changes in the main demand and redirection of complaints of patients during the period of COVID-19.
Social anxiety in digital learning environments: an international perspective and call to action
Authors: Ifenthaler Dirk , Cooper Martin , Daniela Linda , Sahin Muhittin 
Published: August 2023

AbstractThe research focused on digital learning environments has identified various challenges for learners, such as technical problems, lack of community, motivation, self-regulation, self-efficacy, and social anxiety. Social anxiety is conceptualized as an emotional disorder that may impede achievement in higher education. The project reported here investigates N = 666 students' social anxiety in digital learning environments at four higher education institutions located in Australia, Germany, Latvia, and Turkey. This range of contexts allowed the research to cover a wide variety of cultural and institutional idiosyncrasies. Findings revealed different levels of social anxiety in higher education digital learning environments across countries and their cultural contexts. In addition, gender plays a significant role in social anxiety for peer interactions with female students reporting higher social anxiety than male students. The findings suggest that it is worth tertiary educators pausing to consider social anxiety's role in reducing interactions within digital learning environments. Additional research is required to establish the causes of social anxiety in digital learning environments and, as a result, to develop strategies to minimise its effect.
Graphical Abstract
Intrinsic conflicts within ethnic and religious issues in France
Authors: Zhang Jinling 
Published: August 2023

AbstractIn contemporary France, there are intense conflicts that involve both ethnic and religious issues. These conflicts provide an opportunity to study the many challenges involved in the construction of national communities and the governance of nation-states in the West. In the case of France, the continued exacerbation of the ethnic and religious problems is undeniably rooted in a range of factors both historical and current. However, the fundamental factor is that certain dominant ideologies and their value claims clash with the diverse and pluralistic nature of contemporary French society. To a certain extent, the disciplining on French society from these ideologies has distorted and contradicted it across multiple levels. Closely intertwined with ethnicity and religion, these issues predominantly manifest in the Muslim community. The notion of the “civic nation” in particular persistently excludes the notion of the “ethnic nation”. As a result, Muslims face identity dilemmas as an ethnic minority, leading to ethnic conflicts. Furthermore, in France, religious practices continue to deconstruct secular principles. This results in a crisis of secularism and further exacerbates the dispute between Muslims and indigenous society. These two fundamental ideologies, along with numerous associated conflicts, have profoundly influenced how ethnic and religious issues evolved in France, posing a significant challenge to France’s national identity.
Analytical solution of thermal effect on unsteady visco-elastic dusty fluid between two parallel plates in the presence of different pressure gradients
Authors: Elshabrawy Mohamed , Khaled Osama , Abbas Wael , Beshir Salah-Eldeen , Abdeen Mostafa 
Published: August 2023

Abstract
Background
Thermal diffusion of dusty fluids has valuable interference in various fields, including waste-water treatment, oil transportation, and power plant pipes. Dusty fluids are used in lots of industrial fields as a result of their improved heat transfer and heat management capabilities. These industries range from renewable energy systems to aerobic plastic sheet extrusion, manufacturing, and rolling and reaching metal sheet cooling.

Results
The work embodied in this paper presents the analytical solution performed to predict the effects of thermal diffusion on dusty, viscous, incompressible fluid flows between two porous, parallel vertical plates with a heat source or a heat sink. The mathematical equations are solved by the separation of variables and Laplace transform techniques. The influence of temperature is investigated for various values of Prandtl number and heat source or heat sink parameters. Also, the influences of various coefficients like the thermal diffusion coefficient, Schmidt number, Prandtl number, and heat source or heat sink coefficient on the concentration are observed. The fluid velocity distribution is graphically obtained. The solutions are discussed and exhibited graphically. The influences of the thermal diffusion parameter and chemical reaction parameter on fluid and dust particles’ velocities are examined. A parametric study on the effect of time on temperature and concentration is made.

Conclusions
The exact expressions for temperature, concentration, and velocity variation for fluid and dusty particles are obtained analytically. The temperature is inversely proportional to both the Prandtl number Pr and the heat source or heat sink parameter $$H_s$$

H
s

. The concentration of the fluid is inversely proportional to the thermal diffusion parameter Td and the heat source or heat sink parameter $$H_s$$

H
s

.
Key steps and suggestions for a promising approach to a critical care mentoring program
Authors: De Rosa Silvia , Battaglini Denise , Bennett Victoria , Rodriguez-Ruiz Emilio , Zaher Ahmed Mohamed Sabri , Galarza Laura , Schaller Stefan J. ,   on behalf of the NEXT Committee of the ESICM
Published: August 2023

AbstractIn critical care medicine, where there is a demanding career with a problematic work-life balance, mentoring is an important support tool to grow professionally, creating a network of support throughout the career. The mentoring process consists of evidence-based steps to guide critical care mentors and mentees and pair them with each other according to the correct selection and matching of participants.In order to focus on the active role of a young intensivist selected as a mentee at any level and to support their success in a mentoring relationship, the NEXT Committee of the European Society of Intensive Care Medicine (ESICM) developed 2012 a mentoring program.The critical steps of the mentoring program start from establishing a policy and program objectives, passing through the selection of participants, and matching with mentors up to the definition of the personal development plan supported by checklists, worksheets, and evaluation forms. The present manuscript provides key steps and tips for a good, essential based on our experience in the ESICM NEXT-Mentoring Program so that they guide for future mentoring programs conducted by other scientific societies. In addition, we discuss common challenges and how to avoid them.
Left ventricular pseudo-false aneurysm perforating the right ventricle: two case reports
Authors: Moriuchi Hiroki , Koide Masaaki , Kunii Yoshifumi , Tateishi Minori , Maeda Takuya , Sone Kumiko 
Published: August 2023

Abstract
Background
Left ventricular (LV) pseudo-false aneurysm is a rare and fatal complication secondary to myocardial infarction. Sometimes, it may perforate the right ventricle (RV) and lead to acute heart failure. We experienced 2 cases of surgical repair of an LV pseudo-false aneurysm perforating the RV.

Case presentation
Case 1: A 76-year-old man was referred to our hospital due to dyspnea. Echocardiography revealed an LV pseudo-false aneurysm (25 mm × 20 mm) that had perforated the RV. Via LV incision, the two small orifices communicating to the RV were detected and closed by direct suture. A double patch of bovine pericardium and a dacron sheet was sutured around the aneurysm with everting mattress. Case 2: A 51-year-old man, who had undergone percutaneous coronary intervention 1 month before, was referred to our hospital due to an LV aneurysm perforating the RV. Via LV incision, a double-layered patch was sutured around the aneurysm with everting mattress. The communicating hole to RV was closed by bovine pericardium patch with a running suture via an RV incision. Postoperative course was uneventful in both cases.

Conclusions
An LV pseudo-false aneurysm perforating the RV should be considered for urgent repair before serious complications arise and the patient’s general condition deteriorates.
Quantifying acute kidney injury in an Ischaemia-Reperfusion Injury mouse model using deep-learning-based semantic segmentation in histology
Authors: Luchian Andreea , Cepeda Katherine Trivino , Harwood Rachel , Murray Patricia , Wilm Bettina , Kenny Simon , Pregel Paola , Ressel Lorenzo 
Published: August 2023

ABSTRACT
This study focuses on ischaemia-reperfusion injury (IRI) in kidneys, a cause of acute kidney injury (AKI) and end-stage kidney disease (ESKD). Traditional kidney damage assessment methods are semi-quantitative and subjective. This study aims to use a convolutional neural network (CNN) to segment murine kidney structures after IRI, quantify damage via CNN-generated pathological measurements, and compare this to conventional scoring. The CNN was able to accurately segment the different pathological classes, such as Intratubular casts and Tubular necrosis, with an F1 score of over 0.75. Some classes, such as Glomeruli and Proximal tubules, had even higher statistical values with F1 scores over 0.90. The scoring generated based on the segmentation approach statistically correlated with the semiquantitative assessment (Spearman’s rank correlation coefficient=0.94). The heatmap approach localised the intratubular necrosis mainly in the outer stripe of the outer medulla, while the tubular casts were also present in more superficial or deeper portions of the cortex and medullary areas. This study presents a CNN model capable of segmenting multiple classes of interest, including acute IRI-specific pathological changes, in a whole mouse kidney section and can provide insights into the distribution of pathological classes within the whole mouse kidney section.