bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single vesicle analysis reveals the release of tetraspanin positive extracellular vesicles into circulation with high intensity intermittent exercise
Authors: McIlvenna Luke C. , Parker Hannah‐Jade , Seabright Alex P. , Sale Benedict , Anghileri Genevieve , Weaver Samuel R.C. , Lucas Samuel J.E. , Whitham Martin 
Published: March 2023

AbstractSmall extracellular vesicles (sEVs) are released from all cell types and participate in the intercellular exchange of proteins, lipids, metabolites and nucleic acids. Proteomic, flow cytometry and nanoparticle tracking analyses suggest sEVs are released into circulation with exercise. However, interpretation of these data may be influenced by sources of bias introduced by different analytical approaches. Seven healthy participants carried out a high intensity intermittent training (HIIT) cycle protocol consisting of 4 × 30 s at a work‐rate corresponding to 200% of individual max power (watts) interspersed by 4.5 min of active recovery. EDTA‐treated blood was collected before and immediately after the final effort. Platelet‐poor (PPP) and platelet‐free (PFP) plasma was derived by one or two centrifugal spins at 2500 g, respectively (15 min, room temperature). Platelets were counted on an automated haemocytometer. Plasma samples were assessed with the Exoview R100 platform, which immobilises sEVs expressing common tetraspanin markers CD9, CD63, CD81 and CD41a on microfluidic chips and with the aid of fluorescence imaging, counts their abundance at a single sEV resolution, importantly, without a pre‐isolation step. There was a lower number of platelets in the PFP than PPP, which was associated with a lower number of CD9, CD63 and CD41a positive sEVs. HIIT induced an increase in fluorescence counts in CD9, CD63 and CD81 positive sEVs in both PPP and PFP. These data support the concept that sEVs are released into circulation with exercise. Furthermore, platelet‐free plasma is the preferred, representative analyte to study sEV dynamics and phenotype during exercise.
imageKey points
Small extracellular vesicles (sEV) are nano‐sized particles containing protein, metabolites, lipid and RNA that can be transferred from cell to cell.
Previous findings implicate that sEVs are released into circulation with exhaustive, aerobic exercise, but since there is no gold standard method to isolate sEVs, these findings may be subject to bias introduced by different approaches.
Here, we use a novel method to immobilise and image sEVs, at single‐vesicle resolution, to show sEVs are released into circulation with high intensity intermittent exercise.
Since platelet depletion of plasma results in a reduction in sEVs, platelet‐free plasma is the preferred analyte to examine sEV dynamics and phenotype in the context of exercise.
Simulating the Evolution of Non-Metallic Inclusions During the Forging Process
Authors: Mackey Brandon T. , Siegmund Thomas , Sangid Michael D. 
Published: March 2023

AbstractRadial forging of metallic materials requires both high temperatures and large plastic deformation. During this process, non-metallic inclusions (NMIs) can debond from the metallic matrix and break apart, resulting in a linear array of smaller inclusions, known as stringers. The evolution of NMIs into stringers can result in matrix load shedding, localized plasticity, and stress concentrations near the matrix–NMI interface. Due to these factors, stringers can be detrimental to the fatigue life of the final forged component, especially when present near a free surface. By performing a finite element model of the forging process with cohesive zones to simulate material debonding, we contribute to the understanding of processing-induced deformation and damage sequences on the onset of stringer formation for both Type 1 and Type 2 alumina NMIs in a Ni–200 matrix. Through a parametric study, the interactions of forging temperature, strain rate, strain per pass, and interfacial decohesion on the NMI damage evolution metrics are studied, specifically NMI particle separation, rotation, and cavity formation. For Type 2 alumina NMIs, embedded in a Ni–200 matrix, the simulations indicate that at temperatures below 800 °C, particle separation dominates the NMI damage sequences, whereas at temperatures between 900 °C and 1000 °C, below an interfacial bond strength of 178 MPa, cavity formation is the dominate damage evolution mechanism, resulting in matrix load shedding and stress concentrations around the NMI.
A review of lethal thermal tolerance among freshwater mussels (Bivalvia: Unionida) within the North American faunal region
Authors: Fogelman Kaelyn J. , Archambault Jennifer M. , Irwin Elise , Walsh Maureen , Brewer Shannon , Stoeckel James A. 
Published: March 2023

Freshwater mussels of the order Unionida are currently one of the most imperiled groups of organisms in the North American faunal region. Accurate risk assessments and development of effective management strategies for remaining populations require knowledge of thermal limits in the face of increasing surface water temperature due to climate change and various anthropogenic factors. We conducted a systematic literature review of unionid mussels (order Unionida, families Margaritiferidae and Unionidae) in the North American faunal region to (1) summarize lethal thermal tolerance data by life stage and taxonomy, (2) discuss ecological and climate change implications of existing lethal tolerance data, and (3) identify needs for future research. We identified lethal tolerance estimates for only 28 of 302 species in the families Unionidae and Margaritiferidae. The mean acute median lethal temperatures were 32.8 °C for glochidia (19 species), 35.0 °C for juveniles (13 species), and 36.3 °C for adults (4 species). Generally, glochidia were less tolerant than juveniles or adults of the same species—but there were several exceptions. Generally, Amblemini had the highest acute and chronic thermal tolerance of all tribes followed by Anodontini, Pleurobemini, Lampsilini, and Quadrilini. Acclimation temperature affected lethal tolerance endpoints in less than half (52 of 145) of comparisons within species. Lethal tolerance data for additional species, combined with a comprehensive database of in situ surface water temperatures, would be useful for modeling the frequency and duration of lethal limit exceedance in North America and identifying populations currently living at or near their upper lethal limits.
Estimating the Lowest Latitude of Baroclinic Growth
Authors: Peles Oren , Lachmy Orli 
Published: March 2023

Abstract
Midlatitude storm tracks are the most prominent feature of the midlatitude climate. The equatorward boundary of the storm tracks marks the transition from the dry subtropics to the temperate midlatitudes. This boundary can be estimated as the lowest latitude of efficient baroclinic growth. Scaling theories for the lowest latitude of baroclinic growth were previously suggested based on the domain-averaged parameters of the Eady growth rate and supercriticality. In this study, a new estimate for the lowest latitude of baroclinic growth is proposed, based on the assumption that baroclinic growth is limited by the vertical scale of eddy fluxes. An equation for the eddy displacement flux is obtained from which the vertical scale is calculated, given the zonal-mean zonal wind and temperature profiles. It is found that the vertical scale of the eddy displacement flux and the observed baroclinic conversion rate decrease rapidly toward the equator around the same latitude. The seasonal cycle of the lowest latitude of baroclinic growth, calculated from the observed baroclinic conversion rate, is compared with the theoretical estimates. The estimates based on the vertical scale of the eddy displacement flux and supercriticality agree well with the observed lowest latitude of baroclinic growth. In contrast, the estimate based on the Eady growth rate is located around 10°–15° equatorward. The estimate of the lowest latitude of baroclinic growth may be used in future studies for explaining variations in the properties of the storm track, the Hadley cell edge, and the subtropical jet.

Significance Statement
The lowest latitude of baroclinic growth marks the transition from the dry and stable subtropics to the moist and variable midlatitudes. Estimating this latitude based on mean-flow variables can potentially advance the theoretical understanding of the latitudinal structure of the atmospheric circulation around the subtropics and midlatitudes. This study suggests a new method for estimating the lowest latitude of baroclinic growth, which is found to predict the observed lowest latitude of baroclinic energy conversion relatively well, compared with the traditional prediction based on the Eady growth rate.
Intermodel Spread of Historical Indian Monsoon Rainfall Change in CMIP6: The Role of the Tropical Pacific Mean State
Authors: Guilbert Marcellin , Terray Pascal , Mignot Juliette 
Published: March 2023

Abstract
Robust projections of the Indian summer monsoon rainfall (ISMR) are critical as it provides 80% of the annual precipitation to more than 1 billion people who are very vulnerable to climate change. However, even over the historical period, state-of-the-art climate models have difficulties in reproducing the observed ISMR trends and are affected by a large intermodel spread, which questions the reliability of ISMR projections. Such uncertainty could come from internal variability or model biases. Here, we study the impact of the latter on the historical forced change of ISMR in 34 models from CMIP6. First, we show that models’ biases over India do not significantly impact how they simulate the historical change of ISMR. However, we do find statistically significant relationships between ISMR historical forced changes and remote rainfall and temperature biases within the tropics by using a maximum covariance analysis (MCA). Our results highlight the key role of tropical Pacific sea surface temperature (SST) mean state biases as an important source of intermodel spread in the ISMR change. The physical mechanisms underlying these statistical relationships between ISMR change and the intermodel spread of Pacific SST biases are finally explored. We found that models having El Niño/La Niña–like mean SST bias in the Pacific tend to exhibit El Niño/La Niña–like changes over the historical period, impacting ISMR through a shift in the Walker circulation and Rossby wave propagation across the Pacific.
Developing a Methodology for Model Intercomparison and Its Application to Improve Simulated Streamflow by Land Surface Models
Authors: Tinumbang Aulia Febianda Anwar , Yorozu Kazuaki , Tachikawa Yasuto , Ichikawa Yutaka 
Published: March 2023

Abstract
Runoff generated by land surface models (LSMs) is extensively used to predict future river discharge under global warming. However, the structural bias of LSMs, the precipitation bias of the climate model, and other factors could cause the runoff to be biased. A model intercomparison study can help understand LSM behavior. Traditional model intercomparison can discover output variation and evaluate performance, but explaining the reason for the difference is challenging. This study developed a novel method to identify the reasons for disparities and suggest improvements. Consequently, we investigated the impacts of model settings by adopting the settings of another model in one model until it can mimic similar features in its output. Hence, we developed a process called the “emulation model.” We employed two LSMs [Simple Biosphere with Urban Canopy (SiBUC) and Meteorological Research Institute Simple Biosphere model (MRI-SiB)] in the Thai River basin. SiBUC produced a higher surface runoff than MRI-SiB, and the development of the MRI-SiB emulation revealed the cause of this variation. The differences in runoff characteristics affected streamflow estimation. For instance, the SiBUC peak discharge was faster and higher than observed in the dry year. Conversely, there was a tendency to underestimate the flow estimated by MRI-SiB runoff during the transition from dry to wet seasons. Incorporating other model settings can alleviate the shortcomings of each model. Overall, the proposed method can identify the strengths and weaknesses of a model and enhance the reproducibility of the hydrological characteristics of the observed discharge in the basin.

Significance Statement
This study aims to develop a new methodology for model intercomparison to identify the reasons for model output variation. Understanding why models behave differently is important to enhancing the reliability of model prediction. Our findings guide what affects disparities in land surface model runoff-based streamflow estimation, which will help reduce the uncertainty of future flood and drought predictions.
A Rare Case of Extensive Basal Cell Carcinoma With Invasion of the Humerus: Excision and Immediate Reconstruction With Fibula Allograft, Plating, and Latissimus Dorsi Flap
Authors: Nakao Yuki , Fazalare Brandon , Feyh Andrew , Bown Paul , Ray Peter 
Published: March 2023

Basal cell carcinoma (BCC) is the most common skin cancer, and most of the reports have involved the head and neck, but it is rare for it to be highly invasive, with an invasion of long bone being extremely rare. A 73 year old woman presented with a giant BCC on her right arm. Magnetic resonance imaging suggested the involvement of the right humerus. Biopsy confirmed the nodular type of BCC. The patient underwent BCC excision including hemicortical humerus excision with fibula allograft and latissimus dorsi flap with a split-thickness skin graft. Excluding a transient radial nerve palsy, the patient’s postoperative course was otherwise uncomplicated. Although BCC invasion into the long bone is extremely rare, the gold standard treatment is, as a rule, en bloc surgical resection with a wide variety of reconstructive techniques. This treatment is only possible through the collaboration of general surgery, orthopedics, and plastic surgery.
The Global Campaign Against Headache and its future relation to IHS and WHO
Authors: Olesen Jes , Jensen Rigmor Højland 
Published: March 2023

Background Preparations for the Global Campaign Against Headache done jointly with WHO started almost 25 years ago. It was officially incorporated 18 years ago. It is the story of a few dedicated individuals who, together with the World Health Organization, generated epidemiological data all over the world and made use of these data to gradually increase the position of headache disorders, until migraine became number two among all causes of years lost to disability. It is also the story of impressive development of scientific tools, their validation and use in low-income countries. Methods Models of care for headache patients were developed that are adaptable and hence can be implemented in future throughout the world. Results The last phase of the campaign shall use the impressive data set generated to cause real improvement in the healthcare for people with headache throughout the world. The recent World Health Organization initiative: Intersectoral Global Action Plan on Epilepsy and Other Neurological Disorders invites international organizations to collaborate, and headache is mentioned. Conclusion This calls for the International Headache Society and all its national members and all other organizations involved in headache to work together with Lifting the Burden, the organization in charge of the Global Campaign Against Headache.
Treatment pattern and response for cluster headache in Korea: A prospective multicenter observation study
Authors: Lee Mi Ji , Park Jeong Wook , Chu Min Kyung , Moon Heui-Soo , Chung Pil-Wook , Chung Jae Myun , Sohn Jong-Hee , Kim Byung-Kun , Kim Byung-Su , Kim Soo-Kyoung , Song Tae-Jin , Choi Yun-Ju , Park Kwang-Yeol , Oh Kyungmi , Ahn Jin-Young , Lee Kwang-Soo , Bae Dae Woong , Cho Soo-Jin 
Published: March 2023

Background Only limited data are available regarding the treatment status and response to cluster headache in an Asian population. Therefore, this study aimed to provide a real-world treatment pattern of cluster headache and the response rate of each treatment in an Asian population. Methods Patients with cluster headache were recruited between September 2016 and January 2019 from 16 hospitals in Korea. At the baseline visit, we surveyed the patients about their previous experience of cluster headache treatment, and acute and/or preventive treatments were prescribed at the physician’s discretion. Treatment response was prospectively evaluated using a structured case-report form at 2 ± 2 weeks after baseline visit and reassessed after three months. Results Among 295 recruited patients, 262 experiencing active bouts were included. Only one-third of patients reported a previous experience of evidence-based treatment. At the baseline visit, oral triptans (73.4%), verapamil (68.3%), and systemic steroids (55.6%) were the three most common treatments prescribed by the investigators. Most treatments were given as combination. For acute treatment, oral triptans and oxygen were effective in 90.1% and 86.8% of the patients, respectively; for preventive treatment, evidence-based treatments, i.e. monotherapy or different combinations of verapamil, lithium, systemic steroids, and suboccipital steroid injection, helped 75.0% to 91.8% of patients. Conclusion Our data provide the first prospective analysis of treatment responses in an Asian population with cluster headache. The patients responded well to treatment despite the limited availability of treatment options, and this might be attributed at least in part by combination of medications. Most patients were previously undertreated, suggesting a need to raise awareness of cluster headache among primary physicians.
Large Versus Small Opening Wedge High Tibial Osteotomies Performed With a Protective Wire Over the Lateral Hinge: Incidence of Lateral Hinge Fracture and Early Clinical Outcomes
Authors: Koh Don Thong Siang , Soong Junwei , Yeo William , Tan Marcus Wei Ping , Teo Shao Jin , Wilson Adrian , Lee Kong Hwee 
Published: March 2023

Background: The incidence of lateral hinge fractures (LHFs) during medial opening wedge high tibial osteotomy (MOW-HTO) is unacceptably high, especially with distractions >10 mm. LHFs result in malunion, loss of correction, and recurrence of symptoms adversely affecting clinical outcomes. Purpose: (1) To investigate the incidence of LHF when a protective guide wire is utilized during MOW-HTO in small and large corrections and (2) to study the effect of correction size on early clinical outcomes. Study Design: Cohort study; Level of evidence, 3. Methods: A retrospective analysis was performed of 96 knees that underwent MOW-HTO between 2019 and 2020. A protective wire applied intraoperatively across the lateral hinge point before opening wedge distraction was performed for all patients. Patients were divided into 2 groups based on opening wedge sizes: group A (opening distraction <10 mm) and group B (opening distraction ≥10 mm). LHF and wound complications were recorded. Prospective Knee Score and Function Score (Knee Society), Oxford Knee Score, and Physical and Mental Component Summaries of the 36-Item Short Form Health Survey questionnaire were recorded preoperatively and at 6 months and 2 years after surgery. Results: Incidence of LHF was low in group A (n = 2; 6.1%) and group B (n = 3; 9.1%). A single case of intraoperative LHF was noted in each group, with each case resulting in a type 1 fracture. The incidence of postoperative fractures was comparable between groups (groups A vs B, n = 1 vs 2). At 6 months, clinical outcomes in group A were superior to those of group B (Knee Score, 85.7 ± 14.7 vs 73.1 ± 20.3, P = 0.028; Function Score, 73.5 ± 16.5 vs 63.1 ± 19.5, P = 0.047; Oxford Knee Score, 20.2 ± 4.7 vs 25.6 ± 8.5, P = 0.008; Physical Component Summary, 46.8 ± 8.1 vs 40.2 ± 10.9, P = 0.018). However, clinical outcomes were comparable at 2 years ( P > .05). Conclusion: A protective wire was associated with a low incidence of LHF, even in larger MOW-HTO corrections. Large corrections had poorer clinical outcomes as compared with small corrections at 6 months. However, clinical outcomes between groups were comparable at 2 years.
A Collaborative-Care Telephone-Based Intervention for Depression, Anxiety, and at-Risk Drinking in Primary Care: The PARTNERs Randomized Clinical Trial
Authors: Ishrat Husain M. , Rodie David J. , Perivolaris Athina , Sanches Marcos , Crawford Allison , Fitzgibbon Kyle P. , Levinson Andrea , Geist Rose , Kurdyak Paul , Mitchell Brian , Oslin David , Sunderji Nadiya , Mulsant Benoit H. ,   for the PARTNERs Study Group
Published: March 2023

Background Collaborative care (CC) could improve outcomes in primary care patients with common mental conditions. We assessed the effectiveness of a transdiagnostic model of telephone-based CC (tCC) delivered by lay providers to primary care patients with depression, anxiety, or at-risk drinking. Methods PARTNERS was a pragmatic trial in 502 primary care adults presenting with depressive symptoms, anxiety symptoms, or at-risk drinking randomized to (1) usual care by primary care providers (PCPs) enhanced with the results of computer-assisted telephone-based assessments (at baseline and 4, 8, and 12 months later) (enhanced usual care [eUC]) or (2) tCC consisting of eUC plus frequent telephone coaching and psychoeducation provided by mental health technicians who also communicated to the PCP recommendations from a psychiatrist for evidence-based pharmacotherapy, psychotherapy, or, when indicated, referrals to mental health services. The primary analysis compared the change on the 9-item Patient Health Questionnaire (PHQ-9) in participants presenting with depression ( n = 366) randomized to tCC versus eUC. Secondary analyses compared changes on the Generalized Anxiety Disorder-7 scale (GAD-7) in those presenting with anxiety ( n = 298); or change in the number of weekly drinks in those presenting with at-risk drinking ( n = 176). Results There were no treatment or time×treatment effects between tCC and eUC on PHQ-9 scores for patients with depression during the 12-month follow-up. However, there was a treatment effect (tCC > eUC) on GAD-7 scores in those with anxiety and a time×treatment interaction effect on the number of weekly drinks (tCC > eUC) in those with at-risk drinking. Conclusion Implementing transdiagnostic tCC for common mental disorders using lay providers appears feasible in Canadian primary care. While tCC was not better than eUC for depression, there were some benefits for those with anxiety or at-risk drinking. Future studies will need to confirm whether tCC differentially benefits patients with depression, anxiety, or at-risk drinking.