bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of dignity therapy in the context of culturally competent care in people with palliative care needs: a systematic review of systematic reviews
Authors: Johnston Bridget , Dönmez C. Fulya , Julião Miguel 
Published: July 2023

Purpose of review
This review aims to synthesise the evidence from systematic reviews and meta-analyses on the efficacy of dignity therapy (DT) in relation to psychosocial and spiritual outcomes in the context of person-centred and culturally competent care for people with supportive and palliative care needs.

Recent findings
Thirteen reviews were found, including seven conducted by nurses. Most reviews were of high quality, including various study populations such as cancer, motor neurone disease and non-malignant conditions. Six psychosocial and spiritual outcomes were identified: quality of life, anxiety, depression, hopefulness, meaning and purpose in life, and suffering based on the cultural variations in the implementation of DT.

Summary
DT has a positive impact on anxiety, depression, suffering, and meaning and purpose in life for people with palliative care needs, but the evidence is somewhat conflicted as to whether DT is effective in improving hope, quality of life and spiritual outcomes in the context of culturally competent care. Nurse-led DT seems desirable given its pivotal role when caring for people with palliative care needs. More randomised controlled trials should be conducted for people with different cultural backgrounds to provide person-centred, culturally competent supportive and palliative care.
The macronutrient content of a meal modulates subsequent ‘dessert’ intake, via Fibroblast Growth Factor 21 (FGF21)
Authors: Wu Chih-Ting , Larson Karlton R. , Sims Landon C. , Ryan Karen K. 
Published: July 2023

AbstractPharmacological administration of Fibroblast growth factor 21 (FGF21) alters food choice, including that it decreases the consumption of sucrose and other sweet tastants. Conversely, endogenous secretion of FGF21 by the liver is modulated by diet, such that plasma FGF21 is increased after eating foods that have a low dietary protein: total energy (P: E) ratio. Together, these findings suggest a strategy to promote healthy eating, in which the macronutrient content of a pre-load meal could reduce the later consumption of sweet desserts. Here, we tested the prediction that individuals eating a low P: E pre-load meal, and next offered a highly palatable sweet ‘dessert’, would eat less of the sugary snack compared to controls, due to increased FGF21 signaling. In addition to decreasing sweet intake, FGF21 increases the consumption of dietary protein. Thus, we predicted that individuals eating a low protein pre-load meal, and subsequently offered a very high-protein pellet as ‘dessert’ or snack, would eat more of the high protein pellet compared to controls, and that this depends on FGF21. We tested this in C57Bl/6J, and liver-specific FGF21-null (FGF21ΔL) null male and female mice and littermate controls. Contrary to expectation, eating a low protein pre-load did not reduce the later consumption of a sweet solution in either males or females, despite robustly increasing plasma FGF21. Rather, eating the low protein pre-load increased later consumption of a high protein pellet. This was more apparent among males and was abrogated in the FGF21ΔLmice. We conclude that physiologic induction of hepatic FGF21 by a low protein pre-load is not sufficient to reduce later consumption of sweet dessert, though it effectively increases the subsequent intake of dietary protein in male mice.
A reference genome for the long-term kleptoplast-retaining sea slug Elysia crispata morphotype clarki
Authors: Eastman Katharine E. , Pendleton Amanda L. , Shaikh Mearaj A. , Suttiyut Thiti , Ogas Raeya , Tomko Paxton , Gavelis Gregory , Widhalm Joshua R. , Wisecaver Jennifer H. 
Published: July 2023

ABSTRACTSeveral species of sacoglossan sea slugs possess the incredible ability to sequester chloroplasts from the algae they consume. These ‘photosynthetic animals’ incorporate stolen chloroplasts, called kleptoplasts, into the epithelial cells of tubules that extend from their digestive tracts throughout their bodies. The mechanism by which these slugs maintain functioning kleptoplasts in the absence of an algal nuclear genome is unknown. Here, we report a draft genome of the saccoglossan slugElysia crispatamorphotype clarki, a morphotype native to the Florida Keys that can retain photosynthetically active kleptoplasts for several months without feeding. We used a combination of Oxford Nanopore Technologies long reads and Illumina short reads to produce a 786 Mbp assembly containing 68,514 predicted protein-coding genes. A phylogenetic analysis found no evidence of horizontal acquisition of genes from algae. We performed gene family and gene expression analyses to identifyE. crispatagenes unique to kleptoplast-containing slugs that were more highly expressed in fed versus unfed developmental life stages. Consistent with analyses in other kleptoplastic slugs, our investigation suggests that genes encoding lectin carbohydrate-binding proteins and those involved in regulation of reactive oxygen species and immunity may play a role in kleptoplast retention. Lastly, we identified four polyketide synthase genes that could potentially encode proteins producing UV- and oxidation-blocking compounds in slug cell membranes. The genome ofE. crispatais a quality resource that provides potential targets for functional analyses and enables further investigation into the evolution and mechanisms of kleptoplasty in animals.
Deep Learning Approaches to the Phylogenetic Placement of Extinct Pollen Morphotypes
Authors: Adaïmé Marc-Élie , Kong Shu , Punyasena Surangi W. 
Published: July 2023

AbstractThe phylogenetic interpretation of pollen morphology is limited by our inability to recognize the evolutionary history embedded in pollen features. Deep learning offers tools for connecting morphology to phylogeny. Using neural networks, we developed an explicitly phylogenetic toolkit for analyzing the overall shape, internal structure, and texture of a pollen grain. Our analysis pipeline determines whether testing specimens are from unknown species based on uncertainty estimates. Features of novel specimens are passed to a multi-layer perceptron network trained to transform these features into predicted phylogenetic distances from known taxa. We used these predicted distances to place specimens in a phylogeny using Bayesian inference. We trained and evaluated our models using optical superresolution micrographs of 30Podocarpusspecies. We then used trained models to place nine fossilPodocarpiditesspecimens within the phylogeny. In doing so, we demonstrate that the phylogenetic history encoded in pollen morphology can be recognized by neural networks and that deep-learned features can be used in phylogenetic placement. Our approach makes extinction and speciation events that would otherwise be masked by the limited taxonomic resolution of the fossil pollen record visible to palynological analysis.Significance StatementMachine learned features from deep neural networks can do more than categorize and classify biological images. We demonstrate that these features can also be used to quantify morphological differences among pollen taxa, discover novel morphotypes, and place fossil specimens on a phylogeny using Bayesian inference. Deep learning can be used to characterize and identify and morphological features with evolutionary significance. These features can then be used to infer phylogenetic distance. This approach fundamentally changes how fossil pollen morphology can be interpreted, allowing greater evolutionary inference of fossil pollen specimens. The analysis framework, however, is not specific to pollen and can be generalized to other taxa and other biological images.
Meta-analysis of gonadal transcriptome provides novel insights into sex change mechanism across protogynous fishes
Authors: Nozu Ryo , Kadota Mitsutaka , Nakamura Masaru , Kuraku Shigehiro , Bono Hidemasa 
Published: July 2023

AbstractProtogyny, being capable of changing from female to male during their lifetime, is prevalent in 20 families of teleosts but is believed to have evolved within specific evolutionary lineages. Therefore, shared regulatory factors governing the sex change process are expected to be conserved across protogynous fishes. However, a comprehensive understanding of this mechanism remains elusive. To identify these factors, we conducted a meta-analysis using gonadal transcriptome data from seven species. We curated data pairs of ovarian tissue and transitional gonad, and employed ratios of expression level as a unified criterion for differential expression, enabling a meta-analysis across species. Our approach revealed that classical sex change-related genes exhibited differential expression levels between the ovary and transitional gonads, consistent with previous reports. These results validate our methodology’s robustness. Additionally, we identified novel genes not previously linked to gonadal sex change in fish. Notably, changes in the expression levels of acetoacetyl-CoA synthetase and apolipoprotein Eb, which are involved in cholesterol synthesis and transport, respectively, suggest that the levels of cholesterol, a precursor of steroid hormones crucial for sex change, are decreased upon sex change onset in the gonads. This implies a potential universal influence of cholesterol dynamics on gonadal transformation in protogyny.
Modelling the factors affecting organizational flexibility in MSMEs
Authors: Joseph Jerome Jude Jegan , Sonwaney Vandana , O.N. Arunkumar 
Published: July 2023

Purpose
In the era of multiple global disruptions, firms are finding it to continue their business. MSMEs are impacted more as they have constrained resources. Organizational flexibility has emerged as an organizational and management principle that would help firms stay competitive even in volatile markets. This study aims to present a set of guidelines and insights for MSME managers to implement organizational flexibility in their organizations.

Design/methodology/approach
This study uses total interpretive structural modelling to study how the various factors contributing to organizational flexibility behave together. Behavioural theory is used to explain why organizations need to incorporate flexibility, and systems theory of organization is used to explain why an organization needs to have open boundaries.

Findings
Organizational flexibility is a principle that may be supported by the systems theory of organization. The study has shown that it is important for MSMEs to have supply chain collaborations to be more flexible. The study also shows pressure from competitors as the key driver that would make a firm more flexible, and that adequate support from management and technological skills are required to drive flexibility in an organization.

Research limitations/implications
Single respondent bias may have occurred in this study. This can be eliminated by interviewing multiple people from the same organization. Further research around the reasoning for linkages can be explored with theory-driven grounded studies.

Originality/value
This study attempts to use a multi-criteria decision-making technique to present insights to managers to help them make their organizations flexible.
The effect of corporate social responsibility on open innovation: the moderating role of firm proactiveness
Authors: Cai Wenjun , Gu Jibao , Wu Jianlin 
Published: July 2023

PurposeOpen innovation (OI) is an effective way to achieve firms' sustainable development in emerging markets. This study aims to investigate the effects of business and philanthropic corporate social responsibility (CSR) on OI and the moderating role of firm proactiveness in such relationships. This study also examines the effects of OI on firms' financial and innovation performance.Design/methodology/approachThis study uses multisource data from 688 firms in China, including data from surveys of top managers and objective data. The Tobit model, Poisson model, and ordinary least squares regression are adopted to test the hypotheses.FindingsThe results suggest that business CSR and philanthropic CSR both have positive effects on OI. Proactiveness weakens the positive effect of business CSR on OI, while strengthening the effect of philanthropic CSR on OI. The results also show that OI increases firm innovation and financial performance.Originality/valueCSR enables firms to build wild, deep and trust-based relationships with external actors, which may benefit firms in open search of knowledge. However, it has not received adequate attention in the literature on OI. The findings contribute to the research on OI drivers from the perspective of social activities and enhance the understanding of how different types of CSR and firm proactiveness work together to influence OI.
Do nocturnal birds use acoustic and visual cues to avoid predation by bats?
Authors: GONG Lixin , WU Huan , WANG Zhiqiang , WU Hui , FENG Jiang , JIANG Tinglei 
Published: July 2023

AbstractAnti‐predation strategies are critical to animal survival and are fundamental to deciphering predator–prey interactions. As an important defense strategy, sensory predator detection (such as through acoustic and visual cues) enables animals to assess predation risk and execute predator‐avoidance behavior; however, there are limited studies on the anti‐predation behavior of nocturnal animals. The prey of bats provides an excellent representative system for examining the anti‐predation behavior of nocturnal animals. Here, we broadcasted different types of echolocation calls of the bird‐eating bat Ia io to two wild passerine birds, namely, Zosterops japonicus and Sinosuthora webbiana, that are preyed upon by I. io, and presented the birds with individual bats under different light intensities. The results showed that both bird species were able to perceive the low‐frequency audible portion of the bats’ echolocation calls; however, they did not exhibit escape responses to the acoustic stimuli. In the dark and under moonlit conditions, both bird species were unable to respond to active bats at close range and the birds only exhibited evasive flight behavior when bats approached or touched them. These results suggest that nocturnal passerine birds may not be able to use acoustic or visual cues to detect bats and adopt evasive maneuvers to avoid predation. This work suggests that bat predation pressure may not elicit primary predator‐avoidance responses in nocturnal passerine birds. The results provide new insights into the anti‐predation behavior of nocturnal animals.
SERPINB5 is a prognostic biomarker and promotes proliferation, metastasis and epithelial‐mesenchymal transition (EMT) in lung adenocarcinoma
Authors: He Xiaotian , Ma Yiyang , Huang Zirui , Wang Gongming , Wang Weidong , Zhang Rusi , Guo Guangran , Zhang Xuewen , Wen Yingsheng , Zhang Lanjun 
Published: July 2023

AbstractBackgroundSerine protease inhibitors clade B serpins (SERPINBs) are the largest subclass of protease inhibitors, once thought of as a tumor suppressor gene family. However, some SERPINBs exhibit functions unrelated to the inhibition of catalytic activity.MethodsThe Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Gene Set Cancer Analysis (GSCA), and cBioPortal databases were utilized to investigate SERPINBs expression, prognostic correlation, and genomic variation in 33 cancer types. We also conducted a comprehensive transcriptome analysis in multiple lung adenocarcinoma (LUAD) cohorts to reveal the molecular mechanism of SERPINB5 in LUAD. Then, qPCR and immunohistochemistry were used to verify the expression and prognostic value of SERPINB5 in LUAD patients. Furthermore, knockdown and overexpression of SERPINB5 in LUAD cell lines were performed to evaluate cell proliferation, migration, invasion, and epithelial‐mesenchymal transition (EMT).ResultsThe expression of SERPINB5 was upregulated and demethylated in LUAD, and its abnormally high expression was significantly correlated with poor overall survival (OS). In addition, the expression of SERPINB5 was analyzed to determine its prognostic value in LUAD and confirmed that SERPINB5 was an independent predictor of LUAD in TCGA and GEO cohorts and qPCR validation with 106 clinical samples. At last, A knockdown of SERPINB5 in LUAD cells reduced proliferation, migration, and EMT. Proliferation, migration, and invasion are promoted by the overexpression of SERPINB5.ConclusionTherefore, SERPINB5 has shown potential as a prognostic biomarker for LUAD, and it may become a potential therapeutic target for lung adenocarcinoma.
Presentation trends, characteristics and outcomes for women with early pregnancy bleeding in the emergency department: A 10‐year data linkage study
Authors: Trostian Baylie , McCloughen Andrea , Fethney Judith , Curtis Kate 
Published: July 2023

BackgroundWomen present to the emergency department (ED) with pregnancy complications including bleeding. They seek investigations, treatment and clear discharge and referral pathways.AimsThe aim was to identify trends, characteristics, ED care and discharge pathways for women who present to the ED with early pregnancy bleeding.MethodsRetrospective data (from 2011 to 2020) were extracted from a regional health district‘s databank. Data were processed, and deterministic linking was used to produce a final data set. Descriptive statistics were used to identify trends and characteristics. Linear and logistic regression models were used to identify factors that influence health service use, outcomes and discharge pathways.ResultsOver the 10 years, there have been almost 15 000 presentations to the ED for early pregnancy bleeding, from approximately 10 000 women, 0.97% of all ED presentations. The frequency of presentations increased by 19.6% over the study period. The average age of women who presented to the ED was 29.1 years, which increased from 28.5 years (2011) to 29.3 (2020). The median length of stay was less than 4 h, and most women were treated and discharged from the ED. One‐third of presentations received neither ultrasound nor pathology, but health service costs increased by 330% from 2014 to 2020.ConclusionsMaternal age is increasing, as is the frequency of ED presentations for early pregnancy bleeding, and both factors increase demands on the ED. Findings from this study may inform strategies to improve current care models and improve quality and safety practices within the ED.
Development of a novel nomogram to predict the risk of severe compensatory sweating following endoscopic thoracic sympathectomy
Authors: Adhami Mohammadmehdi , Bell Roger 
Published: July 2023

AbstractBackgroundsEndoscopic thoracic sympathectomy (ETS) is a permanent and effective treatment for primary hyperhidrosis and facial blushing; however, severe compensatory sweating (SCS) remains a devastating complication. We aimed to (i) construct a nomogram to predict the risk of SCS, and (ii) investigate factors associated with the level of satisfaction.MethodsFrom Jan 2014 to Mar 2020, 347 patients underwent ETS by a single surgeon. These patients were asked to complete an online questionnaire regarding primary symptom resolution, level of satisfaction, and development of compensatory sweating. Multivariable analysis was conducted via logistic regression and ordinal regression to predict SCS and satisfaction level respectively. Nomogram was developed based on significant predictors.ResultsIn total, 298 (85.9%) patients responded to the questionnaire with a mean follow up of 4.9 ± 1.8 years. Significant factors associated with SCS in the nomogram included older age (OR 1.05, 95% CI 1.02–1.09, P = 0.001), primary indication other than palmar hyperhidrosis (OR 2.30, 95% CI 1.03–5.12, P = 0.04), and current smoking (OR 5.91, 95% CI 2.46–14.20, P < 0.001). The area under receiver operating characteristic curve was 0.713. Multivariable analysis revealed that longer follow up (β = −0.201 ± 0.078, P = 0.01), gustatory hyperhidrosis (β = −0.781 ± 0.267, P = 0.003), primary indication other than palmar hyperhidrosis (β = −1.524 ± 0.292, P < 0.001), and SCS (β = −3.061 ± 0.404, P < 0.001) were independently associated with a lower degree of patient satisfaction.ConclusionThe novel nomogram can provide a personalized numerical risk estimate to assist both the clinician and patient weigh the pros and cons as part of the decision‐making process, mitigating the chance of patient dissatisfaction.
Antimicrobials and antiseptics: Lowering effect on ocular surface bacterial flora – A systematic review
Authors: Ferrara Mariantonia , Gatti Francesca , Lockington David , Iaria Antonio , Kaye Stephen , Virgili Gianni , Aragona Pasquale , Semeraro Francesco , Romano Vito 
Published: July 2023

AbstractTopical antimicrobials and antiseptics are used perioperatively to reduce the ocular surface bacteria flora (OSBF) that are involved in the development of post‐operative infectious complications. However, their effectiveness is still a controversial topic. This systematic review, performed according to the PRISMA guidelines and registered in PROSPERO, aims to provide an overview of the efficacy of the agents currently used in peri‐cataract surgery and ‐intravitreal injections (IVI) in lowering the OSBF. Although effective in lowering OSBF, perioperative topical antimicrobials are associated with the risk of resistance development, with no obvious additional benefit compared with topical antisepsis. Conversely, the effectiveness of topical antiseptics before cataract surgery and IVI is strongly supported. Based on the available evidence, perioperative antimicrobials are not recommended, whereas the perioperative use of antiseptics is strongly recommended as prophylactic treatment for lowering the infection due to OSBF. Post‐operative antimicrobials may be considered in eyes at higher risk for infection.
Impact of packaging system on the microbial quality of chilled rabbit meat over 21 days of storage
Authors: Racewicz Przemysław , Biesiada Hanna , Majewski Michał , Ludwiczak Agnieszka , Stanisz Marek , Składanowska‐Baryza Joanna 
Published: July 2023

AbstractThe pace of life is accelerating, so we are seeking ways to optimize the shelf life of products. To achieve this goal, the microbiological quality of rabbit meat was assessed at 7, 14, and 21 days under refrigerated conditions, utilizing two storage methods, modified atmosphere packaging (MAP) and vacuum packaging (VAC). Maintaining high levels of hygiene is critical not only during slaughter but also during subsequent technological processes and meat storage. The research concluded that the MAP method was more effective at extending the shelf‐life of fresh rabbit meat than the VAC method. Additionally, increasing the CO2 concentrations in meat significantly decreased the Pseudomonas bacteria population (after 14 and 21 days of storage). Conversely, the gaseous mixture containing 70% O2 significantly decreased the Enterobacteriaceae population in the sample after 21 days of storage. Moreover, the MAP storage method considerably impeded microbial growth, particularly the total yeast and mold count, lactic acid bacteria count, and Pseudomonas spp. count. This study's findings demonstrate that rabbit meat can be stored for 21 days in a modified atmosphere containing appropriate concentrations of gases such as gaseous carbon dioxide and oxygen.