bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mizumi: Digital Sports Centre
Authors: Busang Trevor Roma 
Published: June 2023

Digital sport is the product of merging electronic technology into sport activities. It is a new generation of social activitise that can be integrated online and globally regardless of where you are. Based on the popular animated series Pokemon, the concept is to have multiple areas with different activities engaging with the target users around the area. The phrase "catch-em-all" usde to refer to the intention to capture the surrounding people and creating a new interactive area for the anime fans. The buildings are divided by three to resemble the three legendary Pokemon Lake Guardians. The spaces are also planned according to the gym leader theme from the game adapted from the anime.
TreeTop
Authors: Chiam Xin Ru 
Published: June 2023

Where healing begins. Every day waking up preparing for work. At 5pm, tiredness, stress and depression are what's left to bring back home. Breezy greenery, ambience of the habitats and interaction between residents and non-residents are experienced along the way back to the respective units at TreeTop. Gusts of wind blow away the tiredness and stress. Chirping of the insets minimise the sound of depression.
DAUR ULANG AIR LIMBAH MENGGUNAKAN REVERSE OSMOSIS UNTUK MAKE UP WATER COOLING TOWER DI PT NUTRICIA INDONESIA SEJAHTERA
Authors: Taufik Hidayat Ramadhon  , Zakijah Irfin  , Agung Nugroho  
Published: June 2023

Penggunaan air bersih di PT Nutricia Indonesia Sejahtera berkisar antara 35-40 m3 sepanjang tahun 2022. Penggunaan air bersih yang berasal dari PDAM dan sumur harus ditekan penggunaannya untuk mengurangi biaya produksi di perusahan tersebut. Unit cooling tower adalah unit yang paling besar menyerap penggunaan air bersih hingga mencapai 21 m3/hari. Salah satu inovasi yang memberikan dampak secara langsung terhadap upaya pengurangan penggunaan air bersih adalah mendaur ulang air limbah yang akan digunakan sebagai make up water cooling tower menggunakan reverse osmosis. Spesifikasi air sebelum masuk proses reverse osmosis memiliki parameter yang harus dipenuhi terutama kandungan TDS dari air limbah tersebut. Air limbah PT Nutricia Indonesia Sejahtera memiliki kandungan TDS sebesar 1225 mg/L. JIka spesifikasi air tersebut langsung diaplikasikan maka akan menyebabkan proses reverse osmosis mengalami permasalahan seperti fouling yang menyebabkan membran menjadi mampat, banyak aliran reject, periode proses cleaning yang cepat, sehingga proses menjadi tidak efisien dan air yang didaur ulang tidak maksimal. Tujuan dari penelitian ini adalah mencegah fouling dengan pretreatment tambahan sebelum memasuki reverse osmosis dengan menggunakan membran Ultra Filtration (UF) jenis hollow fiber. Inovasi ini mampu menurunkan penggunaan air bersih di PT Nutricia Indonesia Sejahtera hingga 5.46 m3/hari.
Anxiety in students with intellectual disabilities: the influence of staff-perceived social acceptance and rejection in the classroom
Authors: Hofmann Verena 
Published: June 2023

IntroductionStudents with intellectual disabilities often exhibit increased anxiety levels, which can be associated with additional limitations and severe emotional distress. This study investigated the role of social acceptance, social rejection, and general functioning in predicting anxiety.MethodsUsing data on 1,125 students in 179 special needs classrooms that was collected at the beginning and end of one school year, multilevel models were used to predict whether future anxiety was associated with prior staff-perceived social acceptance or rejection by classmates, and with general functioning.ResultsWe found that greater staff-perceived social acceptance was related to a decrease in anxiety over the school year, however, no effect was found for rejection. Further, there was no moderating effect of students’ general functioning.DiscussionPositive peer relationships in the classroom appear to contribute to lower anxiety, while negative relationships do not further worsen anxiety. Implications for special needs schools are discussed.
Using the behaviour change wheel and person-based approach to develop a digital self-management intervention for patients with adrenal insufficiency: the Support AI study protocol
Authors: Llahana Sofia , Mulligan Kathleen , Hirani Shashivadan P. , Wilson Stephanie , Baldeweg Stephanie E. , Grossman Ashley , Norton Christine , Sharman Philippa , McBride Pat , Newman Stanton 
Published: June 2023

IntroductionMost patients with Adrenal insufficiency (AI) require lifelong glucocorticoid replacement. They need to increase glucocorticoids during physical illness or major stressful situations and require parenteral hydrocortisone in the event of an adrenal crisis. Patients with AI have impaired quality of life and high mortality; approximately 1 in 6-12 patients are hospitalised at least once/year from a potentially preventable adrenal crisis. Adoption of self-management behaviours are crucial; these include adherence to medication, following “sick day rules” and associated behaviours that aid prevention and treatment of adrenal crisis such as symptom monitoring, having extra tablets, carrying a medical-alert ID and injection kit, and self-injecting when necessary. Current patient education is ineffective at supporting self-management behaviour change or reducing adrenal crisis-related hospitalisations. This research study aims to gain an in-depth understanding of the barriers and enablers to self-management for patients with AI and to develop an evidence-based digital self-management behaviour change intervention.MethodsThe study is conducted in accordance with the MRC Framework for developing complex interventions. Underpinned by the Behaviour Change Wheel (BCW), the Theoretical Domains Framework (TDF), and the Person-Based Approach, this research will be conducted in two phases: Phase 1 will involve a sequential qualitative/quantitative mixed-methods study involving focus group interviews followed by a cross-sectional survey with patients with AI recruited from patient advocacy groups and endocrine clinics in the UK. Phase 2 will develop the Support AI, a website-based digital behaviour change intervention (DBCI) informed by Phase 1 findings to support self-management for patients with AI. The most appropriate behaviour change techniques (BCTs) will be selected utilising a nominal group technique with an Expert Panel of 10-15 key stakeholders. The design of the Support AI website will be guided by the Person-Based Approach using an Agile iterative “think-aloud” technique with 12-15 participants over 3 usability testing iterations.ConclusionA theory- and evidence-based digital behaviour change intervention will be developed which will be tested in a feasibility randomised trial following completion of this study. The projected benefit includes cost-effective health care service (reduced hospitalisations and demand for specialist services) and improved health outcomes and quality of life for patients with AI.
Arsenic pollution and arsenic-resistant bacteria of drying Urmia Salt Lake
Authors: Haghi Morteza , Diznabi Salar H. , Karaboz Ismail , Ersoy Omeroglu Esra 
Published: June 2023

Extensive arsenic contamination of groundwater, rivers, and agricultural farms has led to widespread arsenic poisoning and consequent problems associated with health and economy. In recent years, many studies have been performed to understand bacterial arsenic detoxification and metabolism which has paved the way for bioremediation applications. This study attempted to isolate and characterize arsenic-resistant bacteria from the drying Urmia lake. As a result of the 16S rDNA analysis, six arsenic-resistant strains were identified as the members of Shouchella, Salipaludibacillus, and Evansella genera. For some of the strains, the maximum tolerance concentration for either arsenate or arsenite was considerably high, 320 and 16 mM, respectively. All the strains harbored the arsenate reductase gene (arsC). The arsenate permease (arsB) gene was identified in all strains except in strains S1, S12, and E15. The metabolic genes of respiratory arsenate reductase (arrB) and arsenite oxidase (arxA) were identified in none of these strains. This feature of the strains with the arsC gene region can be exploited to bioremediate arsenic from contaminated areas by using a two-step process. The second step can be carried out by the adsorption of arsenite to iron oxide or by precipitation with sulfide. Today, more and more ecosystems are being destroyed due to anthropogenic pollution, and it is important to reveal the negative effects and solutions of this situation on the ecosystems. So, the current study provides a potential source of bacteria for such studies that implement bioremediation practices to prevent arsenic catastrophe in vicinal territories, and reveals arsenic pollution in Urmia lake.
In vaccinated individuals serum bactericidal activity against B meningococci is abrogated by C5 inhibition but not by inhibition of the alternative complement pathway
Authors: Ispasanie Emma , Muri Lukas , Schmid Marc , Schubart Anna , Thorburn Christine , Zamurovic Natasa , Holbro Thomas , Kammüller Michael , Pluschke Gerd 
Published: June 2023

IntroductionSeveral diseases caused by the dysregulation of complement activation can be treated with inhibitors of the complement components C5 and/or C3. However, complement is required for serum bactericidal activity (SBA) against encapsulated Gram-negative bacteria. Therefore, C3 and C5 inhibition increases the risk of invasive disease, in particular by Neisseria meningitidis. As inhibitors against complement components other than C3 and C5 may carry a reduced risk of infection, we compared the effect of inhibitors targeting the terminal pathway (C5), the central complement component C3, the alternative pathway (FB and FD), and the lectin pathway (MASP-2) on SBA against serogroup B meningococci.MethodsSerum from adults was collected before and after vaccination with the meningococcal serogroup B vaccine 4CMenB and tested for meningococcal killing. Since the B capsular polysaccharide is structurally similar to certain human polysaccharides, 4CMenB was designed to elicit antibodies against meningococcal outer membrane proteins.ResultsWhile only a few pre-vaccination sera showed SBA against the tested B meningococcal isolates, 4CMenB vaccination induced potent complement-activating IgG titers against isolates expressing a matching allele of the bacterial cell surface-exposed factor H-binding protein (fHbp). SBA triggered by these cell surface protein-specific antibodies was blocked by C5 and reduced by C3 inhibition, whereas alternative (factor B and D) and lectin (MASP-2) pathway inhibitors had no effect on the SBA of post-4CMenB vaccination sera.DiscussionCompared to the SBA triggered by A,C,W,Y capsule polysaccharide conjugate vaccination, SBA against B meningococci expressing a matching fHbp allele was remarkably resilient against the alternative pathway inhibition.
Effectiveness and safety of anti-IL-5/Rα biologics in eosinophilic granulomatosis with polyangiitis: a two-year multicenter observational study
Authors: Nolasco Santi , Portacci Andrea , Campisi Raffaele , Buonamico Enrico , Pelaia Corrado , Benfante Alida , Triggiani Massimo , Spadaro Giuseppe , Caiaffa Maria Filomena , Scioscia Giulia , Detoraki Aikaterini , Valenti Giuseppe , Papia Francesco , Tomasello Alessandra , Crimi Nunzio , Scichilone Nicola , Pelaia Girolamo , Carpagnano Giovanna Elisiana , Crimi Claudia 
Published: June 2023

BackgroundEosinophilic granulomatosis with polyangiitis (EGPA) is a rare vasculitis characterized by asthma, systemic manifestations, and blood and tissue eosinophilia.ObjectiveTo assess the effectiveness and safety of mepolizumab (anti-IL-5) and benralizumab (anti-IL-5Rα) in EGPA for 24 months.MethodsWe conducted a multicenter observational study, including patients with EGPA treated with anti-IL-5/Rα biologics in 9 Italian specialized facilities. Systemic disease activity, remission and relapse rate were evaluated from 3 to 24 months after treatment initiation. Respiratory outcomes, hematological parameters, corticosteroid (OCS) and immunosuppressants consumption were also assessed.Results49 patients with relapsing-refractory EGPA were included [26 (53.1%) benralizumab 30mg, 20 (40.8%) mepolizumab 100mg, 3 (6.1%) mepolizumab 300mg]. Overall, 38.8% and 57.1% achieved remission after 12 and 24 months, respectively (69.2% benralizumab and 43.5% mepolizumab). Lower OCS intake and higher blood eosinophil count at baseline were associated with remission at 24 months. Both biologics exerted beneficial effects on severe asthma outcomes. Indeed, 61.2% (61.5% benralizumab and 60.8% mepolizumab) remained exacerbation-free during treatment. Lung function parameters showed improvements in the overall cohort (all p&lt;0.05), but began to decline from month 12, especially with mepolizumab. Marked reduction in blood eosinophils was registered with mepolizumab (p&lt;0.0001), while benralizumab depleted both eosinophils (p&lt;0.0001) and basophils (p&lt;0.0001). In general, 69.6% (76% benralizumab and 61.9% mepolizumab) of OCS-dependent patients lowered their daily dose by 75%, while 28.3% discontinued these drugs. Immunosuppressants were suspended in 88.2% of cases. Adverse events were reported in 8.2% of patients.ConclusionsThese real-world data suggest that anti-IL-5/Rα biologics are effective and safe in the long-term as add-on treatments for patients with EGPA.
Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages
Authors: Kim Jin Kyung , Jung Hui-Jung , Hyun Miri , Lee Ji Yeon , Park Jong-Hwan , Suh Seong-Il , Baek Won-Ki , Kim Hyun ah 
Published: June 2023

IntroductionHypervirulent Klebsiella pneumoniae (hvKp) has emerged as a clinically significant global pathogen in the last decade. However, the host immune responses of the macrophages during hvKp infection are largely unknown. In the present study, we aimed to compare the cytotoxic effects of hvKp and classical K. pneumoniae (cKp) in murine macrophages.ResultsWe found that the activation of caspase-1 -dependent pyroptosis was higher in cKp-infected macrophages compared with that in hvKp-infected macrophages. In Caspase-1 deficiency macrophages, pyroptosis diminished during infection. Both hvKp and cKp strains led to nucleotide-binding and oligomerization domain-like receptor protein 3 (NLRP3) inflammasome formation and lysosomal cathepsin B activation, thus resulting in pyroptosis. Compared with the cKp strain, the hvKp strain inhibited these phenomena in murine macrophages.ConclusionHvKp infection resulted in different levels of pyroptosis via the activation of cathepsin B-NLRP3-caspase-1 in murine macrophages. Therefore, the manipulation of pyroptotic cell death is a potential target for host response during hvKp infection in macrophages.
PGE2-EP2/EP4 signaling elicits mesoCAR T cell immunosuppression in pancreatic cancer
Authors: Akbari Behnia , Soltantoyeh Tahereh , Shahosseini Zahra , Jadidi-Niaragh Farhad , Hadjati Jamshid , Brown Christine E. , Mirzaei Hamid Reza 
Published: June 2023

IntroductionFor many years, surgery, adjuvant and combination chemotherapy have been the cornerstone of pancreatic cancer treatment. Although these approaches have improved patient survival, relapse remains a common occurrence, necessitating the exploration of novel therapeutic strategies. CAR T cell therapies are now showing tremendous success in hematological cancers. However, the clinical efficacy of CAR T cells in solid tumors remained low, notably due to presence of an immunosuppressive tumor microenvironment (TME). Prostaglandin E2, a bioactive lipid metabolite found within the TME, plays a significant role in promoting cancer progression by increasing tumor proliferation, improving angiogenesis, and impairing immune cell’s function. Despite the well-established impact of PGE2 signaling on cancer, its specific effects on CAR T cell therapy remain under investigation. MethodsTo address this gap in knowledge the role of PGE2-related genes in cancer tissue and T cells of pancreatic cancer patients were evaluated in-silico. Through our in vitro study, we manufactured fully human functional mesoCAR T cells specific for pancreatic cancer and investigated the influence of PGE2-EP2/EP4 signaling on proliferation, cytotoxicity, and cytokine production of mesoCAR T cells against pancreatic cancer cells.ResultsIn-silico investigations uncovered a significant negative correlation between PGE2 expression and gene signature of memory T cells. Furthermore, in vitro experiments demonstrated that the activation of PGE2 signaling through EP2 and EP4 receptors suppressed the proliferation and major antitumor functions of mesoCAR T cells. Interestingly, the dual blockade of EP2 and EP4 receptors effectively reversed PGE2-mediated suppression of mesoCAR T cells, while individual receptor antagonists failed to mitigate the PGE2-induced suppression.DiscussionIn summary, our findings suggest that mitigating PGE2-EP2/EP4 signaling may be a viable strategy for enhancing CAR T cell activity within the challenging TME, thereby improving the efficacy of CAR T cell therapy in clinical settings.
Changes in patient-reported outcomes in patients with non-idiopathic pulmonary fibrosis fibrotic interstitial lung disease and progressive pulmonary fibrosis
Authors: Takei Reoto , Matsuda Toshiaki , Fukihara Jun , Sasano Hajime , Yamano Yasuhiko , Yokoyama Toshiki , Kataoka Kensuke , Kimura Tomoki , Suzuki Atsushi , Furukawa Taiki , Fukuoka Junya , Johkoh Takeshi , Kondoh Yasuhiro 
Published: June 2023

BackgroundHealth-related quality of life (HRQoL) captures different aspects of the fibrotic interstitial lung disease (FILD) evaluation from the patient’s perspective. However, little is known about how HRQoL changes in patients with non-idiopathic pulmonary fibrosis (IPF) FILD, especially in those with progressive pulmonary fibrosis (PPF). The aim of this study is to clarify whether HRQoL deteriorates in patients with non-IPF FILD and to evaluate the differences in the changes in HRQoL between those with and without PPF.MethodsWe collected data from consecutive patients with non-IPF FILD and compared annual changes in HRQoL over 2 years between patients with PPF and those without. The St George’s respiratory questionnaire (SGRQ) and COPD assessment test (CAT) were used to assess HRQoL. Changes in the SGRQ and CAT scores for 24 months from baseline were evaluated with a mixed-effect model for repeated measures.ResultsA total of 396 patients with non-IPF FILD were reviewed. The median age was 65 years and 202 were male (51.0%). The median SGRQ and CAT scores were 29.6 and 11, respectively. Eighty-six (21.7%) showed PPF. Both SGRQ and CAT scores were significantly deteriorated in patients with PPF compared to those without PPF (p &lt; 0.01 for both). Clinically important deterioration in the SGRQ and CAT scores were observed in 40.0 and 35.7% of patients with PPF and 11.7 and 16.7% of those without, respectively. PPF was significantly associated with clinically important deterioration in the SGRQ score (odds ratio 5.04; 95%CI, 2.61–9.76, p &lt; 0.01) and CAT score (odds ratio 2.78; 95%CI, 1.27–6.06, p = 0.02).ConclusionThe SGRQ and CAT scores were significantly deteriorated in patients with non-IPF FILD and PPF. Considering an evaluation of HRQoL would be needed when assessing PPF.
The association between regional adiposity, cognitive function, and dementia-related brain changes: a systematic review
Authors: Boccara Ethel , Golan Sapir , Beeri Michal Schnaider 
Published: June 2023

BackgroundAdiposity has been previously associated with cognitive impairment and Alzheimer’s disease and related disorders (ADRD). Body mass index (BMI) is the most common measure of global adiposity, but inconsistent results were found since it is a global measurement. BMI does not represent regional fat distribution which differs between sexes, race, and age. Regional fat distribution may contribute differently to cognitive decline and Alzheimer’s disease (AD)-related brain changes. Fat-specific targeted therapies could lead to personalized improvement of cognition. The goal of this systematic review is to explore whether regional fat depots, rather than central obesity, should be used to understand the mechanism underlying the association between adiposity and brain.MethodsThis systematic review included 33 studies in the English language, conducted in humans aged 18 years and over with assessment of regional adiposity, cognitive function, dementia, and brain measures. We included only studies that have assessed regional adiposity using imaging technics and excluded studies that were review articles, abstract only or letters to editor. Studies on children and adolescents, animal studies, and studies of patients with gastrointestinal diseases were excluded. PubMed, PsychInfo and web of science were used as electronic databases for literature search until November 2022.ResultsBased on the currently available literature, the findings suggest that different regional fat depots are likely associated with increased risk of cognitive impairment, brain changes and dementia, especially AD. However, different regional fat depots can have different cognitive outcomes and affect the brain differently. Visceral adipose tissue (VAT) was the most studied regional fat, along with liver fat through non-alcoholic fatty liver disease (NAFLD). Pancreatic fat was the least studied regional fat.ConclusionRegional adiposity, which is modifiable, may explain discrepancies in associations of global adiposity, brain, and cognition. Specific regional fat depots lead to abnormal secretion of adipose factors which in turn may penetrate the blood brain barrier leading to brain damage and to cognitive decline.
Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China
Authors: Li Chunpei , Fan Maopan , Wang Xuan , Li Xue , Zhao Guang , Liu Gangcai , Zhao Jixia 
Published: June 2023

IntroductionRock weathering is crucial in the development of soil. Yet the role of bacteria in the fine particle-forming process of purple mudstone is not fully understood, especially under nitrogen fertilization.MethodsIn this study, the particles (0.25 mm to 1 mm) of purple mudstone from Penglai Group (J3p) were selected as the test material. Two nitrogen fertilizers, i.e., urea (U) and ammonium bicarbonate (AB), and four application levels (0, 280, 560, and 840 N kg∙ha−1) with 18 replications were designed in an incubation experiment. The weathering indices and bacterial community structure of the purple mudstone particles were investigated after 120 days of incubation.ResultsThe results showed that the weathering indices of purple mudstone particles in the AB treatment were higher than that in the U treatment at the same fertilization levels and a reducing trend was observed with increasing nitrogen fertilizer levels under the same nitrogen fertilizer application types. The diversities of the bacterial community were extremely significantly altered by nitrogen fertilizer application (p &lt; 0.01). The effect of the nitrogen fertilizer application level on the beta diversity of the bacterial community (R2 = 0.34) was greater than that of the nitrogen fertilizer application type (R2 = 0.20). Through stepwise regression analysis, the positive effects of nitrification of Nitrobacter (Nitrolancea) (R2 = 0.36), the Phosphorous-dissolving bacteria (Massilia) (R2 = 0.12), and N-NO3− (R2 = 0.35) on the weathering indices of J3p purple mudstone particles could be observed. Structural equation modelling indicated that nitrogen fertilizer application level affects the abundance of the dominant species at the genus level (Nitrolancea and Massilia), and key environmental factor (N-NO3−), which in turn accelerated the weathering indices (59%).Discussion and ConclusionOur findings imply that the enhancements of nitrification of Nitrobacter (Nitrolancea) and of phosphorus solubilization of Phosphorous-dissolving bacteria (Massilia) by nitrogen fertilization are the key factors affecting the weathering indices of J3p purple mudstone particles.