bluuebunny/crossref_metadata_2025_split huggingface dataset:
A dual-flow RootChip enables quantification of bi-directional calcium signaling in primary roots
Authors: Allan Claudia , Tayagui Ayelen , Hornung Rainer , Nock Volker , Meisrimler Claudia-Nicole 
Published: January 2023

One sentence summary: Bi-directional-dual-flow-RootChip to track calcium signatures in Arabidopsis primary roots responding to osmotic stress.Plant growth and survival is fundamentally linked with the ability to detect and respond to abiotic and biotic factors. Cytosolic free calcium (Ca2+) is a key messenger in signal transduction pathways associated with a variety of stresses, including mechanical, osmotic stress and the plants’ innate immune system. These stresses trigger an increase in cytosolic Ca2+ and thus initiate a signal transduction cascade, contributing to plant stress adaptation. Here we combine fluorescent G-CaMP3 Arabidopsis thaliana sensor lines to visualise Ca2+ signals in the primary root of 9-day old plants with an optimised dual-flow RootChip (dfRC). The enhanced polydimethylsiloxane (PDMS) bi-directional-dual-flow-RootChip (bi-dfRC) reported here adds two adjacent inlet channels at the base of the observation chamber, allowing independent or asymmetric chemical stimulation at either the root differentiation zone or tip. Observations confirm distinct early spatio-temporal patterns of salinity (sodium chloride, NaCl) and drought (polyethylene glycol, PEG)-induced Ca2+ signals throughout different cell types dependent on the first contact site. Furthermore, we show that the primary signal always dissociates away from initially stimulated cells. The observed early signaling events induced by NaCl and PEG are surprisingly complex and differ from long-term changes in cytosolic Ca2+ reported in roots. Bi-dfRC microfluidic devices will provide a novel approach to challenge plant roots with different conditions simultaneously, while observing bi-directionality of signals. Future applications include combining the bi-dfRC with H2O2 and redox sensor lines to test root systemic signaling responses to biotic and abiotic factors.
Distribution profile of iridoid glycosides and phenolic compounds in two Barleria species and their correlation with antioxidant and antibacterial activity
Authors: Singh Shachi , Kumar Mukesh , Dwivedi Seema , Yadav Anjali , Sharma Sarika 
Published: January 2023

IntroductionBarleria prionitis is known for its medicinal properties from ancient times. Bioactive iridoid glycosides and phenolic compounds have been isolated from leaves of this plant. However, other parts of a medicinal plants are also important, especially roots. Therefore, it is important to screen all organs for complete chemical characterization.MethodAll parts of B. prionitis, including leaf, root, stem and inflorescence in search of bioactive compounds, with a rapid and effective metabolomic method. X500R QTOF system with information dependent acquisition (IDA) method was used to collect high resolution accurate mass data (HRMS) on both the parent (MS signal) and their fragment ions (MS/MS signal). ESI spectra was obtained in positive ion mode from all parts of the plant. A comparative analysis of antioxidant and antibacterial activity was done and their correlation study with the identified compounds was demonstrated. Principal component analysis was performed.ResultIridoid glycosides and phenolic compounds were identified from all parts of the showing variability in presence and abundance. Many of the compounds are reported first time in B. prionitis. Antioxidant and antibacterial activity was revealed in all organs, root being the most effective one. Some of the iridoid glycoside and phenolic compounds found to be positively correlated with the tested biological activity. Principal component analysis of the chemical profiles showed variability in distribution of the compounds.ConclusionAll parts of B. prionitis are rich source of bioactive iridoid glycosides and phenolic compounds.
Comparative transcriptomic analysis of the gene expression and underlying molecular mechanism of submergence stress response in orchardgrass roots
Authors: Qu Minghao , Zheng Yuqian , Bi Lei , Yang Xingyun , Shang Panpan , Zhou Xiaoli , Zeng Bing , Shen Bingna , Li Wenwen , Fan Yan , Zeng Bing 
Published: January 2023

IntroductionSubmergence stress creates a hypoxic environment. Roots are the first plant organ to face these low-oxygen conditions, which causes damage and affects the plant growth and yield. Orchardgrass (Dactylis glomerata L.) is one of the most important cold-season forage grasses globally. However, their submergence stress-induced gene expression and the underlying molecular mechanisms of orchardgrass roots are still unknown.MethodsUsing the submergence-tolerant ‘Dianbei’ and submergence-sensitive ‘Anba’, the transcriptomic analysis of orchardgrass roots at different time points of submergence stress (0 h, 8 h, and 24 h) was performed.ResultsWe obtained 118.82Gb clean data by RNA-Seq. As compared with the control, a total of 6663 and 9857 differentially expressed genes (DEGs) were detected in Dianbei, while 7894 and 11215 DEGs were detected in Anba at 8 h and 24 h post-submergence-stress, respectively. Gene Ontology (GO) enrichment analysis obtained 986 terms, while Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis obtained 123 pathways. Among them, the DEGs in plant hormones, mitogen-activated protein kinase (MAPK) and Ca2+ signal transduction were significantly differentially expressed in Dianbei, but not in Anba.DiscussionThis study was the first to molecularly elucidate the submergence stress tolerance in the roots of two orchardgrass cultivars. These findings not only enhanced our understanding of the orchardgrass submergence tolerance, but also provided a theoretical basis 36 for the cultivation of submergence-tolerant forage varieties.
Introgression of the QTL qSB11-1TT conferring sheath blight resistance in rice (Oryza sativa) into an elite variety, UKMRC 2, and evaluation of its backcross-derived plants
Authors: Hossain Md. Kamal , Islam Mohammad Rafiqul , Sundaram Raman Meenakshi , Bhuiyan Md. Atiqur Rahman , Wickneswari Ratnam 
Published: January 2023

IntroductionSheath blight (SB) is the most damaging fungal disease in rice caused by a soil-borne pathogenic fungus, Rhizoctonia solani Kuhn (R. solani). The disease resistance in rice is a complex quantitative trait controlled by a few major genes. UKMRC2 is a newly developed elite rice variety that possesses high yield potential but is susceptible to sheath blight disease indicating a huge risk of varietal promotion, mass cultivation, and large-scale adoption. The aim of our present study was the development of varietal resistance against R. solani in UKMRC2 to enhance its stability and durability in a wide range of environments and to validate the effects of an SB-resistance QTL on the new genetic background.MethodsIn our study, we developed 290 BC1F1 backcross progenies from a cross between UKMRC2 and Tetep to introgress the QTL qSBR11-1TT into the UKMRC2 genetic background. Validation of the introgressed QTL region was performed via QTL analysis based on QTL-linked SSR marker genotyping and phenotyping against R. solani artificial field inoculation techniques.Results and DiscussionThe QTL qSBR11-1TT was then authenticated with the results of LOD score (3.25) derived from composite interval mapping, percent phenotypic variance explained (14.6%), and additive effect (1.1) of the QTLs. The QTL region was accurately defined by a pair of flanking markers K39512 and RM7443 with a peak marker RM27360. We found that the presence of combination of alleles, RM224, RM27360 and K39512 demonstrate an improved resistance against the disease rather than any of the single allele. Thus, the presence of the QTL qSBR11-1TT has been validated and confirmed in the URMRC2 genetic background which reveals an opportunity to use the QTL linked with these resistance alleles opens an avenue to resume sheath blight resistance breeding in the future with marker-assisted selection program to boost up resistance in rice varieties.
Better forbearance, lower depression: Evidence based on heart rate variability
Authors: Su Tiehong , Guo Xinwen , Liu Manhua , Xiao Rong , Xiao Zhongju 
Published: January 2023

BackgroundThe relationship between forbearance, a psychological resource, and depression has to date remained inconclusive. The present study investigated heart rate variability (HRV) reactivity to acute stressor tasks in participants with different levels of forbearance to discover how forbearance influences depressive emotions when facing adversity.MethodThe study examined the relationship between forbearance and depression, comparing HRV reactivity to stressor tasks in participants with different levels of forbearance. The levels of reported forbearance were assessed by the Forbearance Scale (FS). The Patient Health Questionnaire-9 (PHQ-9) was used to assessed depression severity. HRV reactivity was evaluated at five stages: baseline, the active stressor task, the period of recovery after the active stressor task, the passive stressor task, the period of recovery after the passive stressor task.ResultsFS scores had a significant negative correlation with PHQ-9 and a significant positive correlation with HRV; significant differences existed between the basal HRV in the higher and lower FS groups. In the passive stressor task and the period of recovery after the active stressor task, significantly different HRV responses were identified between the two groups.DiscussionForbearance was correlated with depression and HRV. The present research found differences in HRV among subjects with different levels of forbearance in the baseline as well as stressor and recovery periods, suggesting that self-regulation dysfunction may exist among persons with lower levels of forbearance. Because of the higher levels of forbearance, the negative emotions of individuals caused by adversity are mitigated.
Dense is not green: How visual density influences greenness evaluation on environmentally friendly products
Authors: Xiao Chunqu , Wang Haoyuan , Zhou Yayu , Li Qingyi 
Published: January 2023

IntroductionThe visual design of environmentally friendly products has a strong influence on consumer decisions. The study offers a novel insight, suggesting that consumers' perceptions of environmentally friendly products may be affected by the visual density design.MethodsFour experiments tested the effect of visual density on the perceived greenness of environmentally friendly products.ResultsStudy 1 showed that perceived greenness was higher for environmentally friendly products with low visual density design. Study 2 repeatedly confirmed this impact and found that perceived production cost acted as a mediating factor. Study 3 and 4 found two boundary conditions for this effect. Study 3 showed that the effect of visual density design attenuated for consumers with weak holistic thinking. Study 4 further revealed that when emphasizing the use of environment-friendly materials, the effect of visual density design was also attenuated.DiscussionThe findings enrich the discussion on the visual design of green products, extend the effect of visual density on consumer attitudes, and provide practical implications for marketers to choose the appropriate appearance for environmentally friendly products.
Struggle for recognition, rights, and redistribution: Understanding the identity of parents of children with autism spectrum disorder in China
Authors: Liu Ying , Fisher Karen R. 
Published: January 2023

IntroductionThe number of children diagnosed with autism spectrum disorders (ASD) worldwide has increased rapidly in the past decade and China is no exception. Yet the identity development of Chinese parents of children with ASD is little understood. This study employed an ethics of care perspective to explore the identity of parents of children with ASD as shaped in their social–cultural context in mainland China.MethodsQualitatively driven mixed-method design was adopted. Qualitative data about their experiences were obtained from in-depth interviews with 20 parents from 17 families of children with ASD in Beijing and participant observation of 9 participants’ daily parenting experience.ResultsA complex and dynamic parenting identity was revealed. With limited recognition within and external to the family, parents experienced constant challenges toward their sense of self. The parents used strategies to assert their rights as carers and develop positive self-perceptions. Yet because of the unjust distribution of care work within families and with the state, the parents retained a sense of insecurity throughout the process of parenting. The parents’ sense of inferiority due to devaluing children with disabilities was accentuated by traditional Chinese cultural values about good parenting. They were intensely worried about the lack of policy for support as they and their children grew older.DiscussionThe findings reinforce the need for recognition of parents’ dignity, capacity, and efforts in caring.
At the heart of change: Differences in young offenders’ HRV patterns after the delivery of the PSYCHOPATHY.COMP program
Authors: Sousa Rúben , Ribeiro da Silva Diana , Petrocchi Nicola , Gilbert Paul , Rijo Daniel 
Published: January 2023

IntroductionLiterature has pointed the need for intervention programs specifically tailored to target the treatment needs of young offenders, as well as the need to test the efficacy of such programs through physiological indexes of emotion regulation (e.g., heart rate variability; HRV), complementing self-reports typically used as outcome measures. The PSYCHOPATHY.COMP is a 20-session individual intervention program based on Compassion Focused Therapy aiming to reduce psychopathic traits and disruptive behavior among young offenders through the development of a compassionate motivation, while stimulating the soothing system as a strategy to improve emotion regulation. Previous research with young offenders has shown decreases in vagally mediated HRV (vmHRV) when the soothing system is activated. This physiological pattern seems to mirror threat-like responses that contrast with relaxed states.MethodsTo test the efficacy of the PSYCHOPATHY.COMP, a clinical trial was implemented encompassing a treatment (n = 56) and a control group (n = 53). Treatment participants attended the PSYCHOPATHY.COMP, while controls received the Treatment As Usual (TAU) delivered in Portuguese juvenile detention facilities. HRV data was collected throughout a standardized procedure (encompassing resting, reactivity and recovery phases) specifically designed to trigger the soothing system. Participants were assessed at pre-treatment, post-treatment and 6-months follow-up.ResultsAlthough treatment participants continued to process the soothing system as unpleasant (with decreased vmHRV), they seem to become able to adaptively recover from the stimuli without avoiding it or resorting to maladaptive coping strategies. The physiological pattern was in line with participants’ decreases in difficulties in emotion regulation across the assessment periods. In contrast, controls seemed to have actively employed coping strategies associated with increases in vmHRV not only when the soothing system was triggered, but also when recovering from the stimuli. Congruently, for controls, increases in difficulties in emotion regulation were found, with increases in the lack of emotional clarity across the assessment periods.DiscussionFindings offer new evidence for the efficacy of the PSYCHOPATHY.COMP program in improving emotion regulation in young offenders, assessed through both self-report and physiological measures. Additionally, findings support the assessment of the autonomic balance as a treatment efficacy index in future research, targeting the rehabilitation of these youth.Clinical trial registrationClinicalTrials.gov, identifier NCT03971682.
The abnormal implicit memory to positive and negative stimuli in patients with current and remitted major depressive disorder: A systematic review and meta-analysis
Authors: Liu Xingze , Wang Xiang , Liu Yao , Gao Feng , Xia Jie , Fan Jie , Zhu Xiongzhao 
Published: January 2023

IntroductionIn patients with current major depressive disorder (cMDD) a general abnormal implicit memory has been reported. However, the elaborate function of implicit memory when processing stimuli with different emotions (i.e., positive, neutral, and negative) in current and remitted (rMDD) patients is unclear. The present review examines implicit memory’s general and elaborate in cMDD and rMDD patients.MethodsWe conducted meta-analyses based on published studies meeting criteria in Web of Science, PubMed, and EMBASE databases between 1990 and July 2022. The full sample patients included cMDD = 601 and rMDD = 143.ResultsInitial analysis of cMDD patients revealed a general implicit memory deficit. Subsequent subgroup analyses showed that the implicit memory performance to neutral stimuli is poorer in cMDD patients than controls, but recovered in rMDD patients; the deficient implicit memory to positive stimuli existed in cMDD and rMDD patients; the implicit memory performance to negative stimuli in cMDD patients is similar to controls but poorer in rMDD patients.ConclusionThese findings indicate that the negative bias in cMDD patients might compensate for the general implicit memory deficit. Together, the implicit memory to neutral stimuli could recover with remission, whereas still abnormal in processing positive and negative stimuli. These results suggested that the abnormal implicit memory to positive and negative information might be relevant to depression pathogenesis.Systematic review registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42020205003.
Neural correlates of emotion acceptance and suppression in borderline personality disorder
Authors: Fernando Silvia Carvalho , Beblo Thomas , Lamers Agnes , Schlosser Nicole , Woermann Friedrich G. , Driessen Martin , Toepper Max 
Published: January 2023

BackgroundEmotion dysregulation is a central feature of borderline personality disorder (BPD). Since impaired emotion regulation contributes to disturbed emotion functioning in BPD, it is crucial to study underlying neural activity. The current study aimed at investigating the neural correlates of two emotion regulation strategies, namely emotion acceptance and suppression, which are both important treatment targets in BPD.MethodsTwenty-one women with BPD and 23 female healthy control participants performed an emotion regulation task during functional magnetic resonance imaging (fMRI). While watching fearful movie clips, participants were instructed to either accept or to suppress upcoming emotions compared to passive viewing.ResultsResults revealed acceptance-related insular underactivation and suppression-related caudate overactivation in subjects with BPD during the emotion regulation task.ConclusionThis is a first study on the neural correlates of emotion acceptance and suppression in BPD. Altered insula functioning during emotion acceptance may reflect impairments in emotional awareness in BPD. Increased caudate activity is linked to habitual motor and cognitive processes and therefore may accord to the well-established routine in BPD patients to suppress emotional experiences.
Identification of genetic risk loci for depression and migraine comorbidity in Han Chinese residing in Taiwan
Authors: Tsai Ming-Chen , Tsai Chia-Lin , Liang Chih-Sung , Lin Yu-Kai , Lin Guan-Yu , Tsai Chia-Kuang , Yeh Po-Kuan , Liu Yi , Hung Kuo-Sheng , Yang Fu-Chi 
Published: January 2023

IntroductionThe genetic association between depression and migraine has not been well investigated in Asian populations. Furthermore, the genetic basis of depression and comorbid migraine subtypes remains nebulous. Hence, in the current study we investigate the susceptibility loci associated with depression and migraine comorbidity in the Han Chinese population in Taiwan.MethodsWe perform a genome-wide association study involving 966 migraine patients, with or without comorbid depression. Genotyping is performed using participant genomic DNA. Association analyses are performed for the entire migraine cohort (subgroups: episodic migraine, chronic migraine, and migraine with or without aura).ResultsResults show that the single nucleotide polymorphism variants of the CDH4 intron region (rs78063755), NTRK3-AS1 downstream region (rs57729223), and between LINC01918 and GPR45 (rs2679891) are suggestively associated with depression. Twenty additional susceptibility loci occur within the subgroups. A multivariate association study demonstrated that a variant in the intron region of CDH4 rs78063755 was associated with Beck Depression Inventory and Migraine Disability Assessment scores.DiscussionThe findings of this study identify several genetic loci suggestively associated with depression among migraine patients in the Han Chinese population. Moreover, a potential genetic basis has been characterized for depression and migraine comorbidity, thus providing genetic candidates for further investigation.
Effectiveness of virtual mindfulness-based interventions on perceived anxiety and depression of physicians during the COVID-19 pandemic: A pre-post experimental study
Authors: Al Ozairi Abdullah , Alsaeed Dalal , Al-Ozairi Ebaa , Irshad Mohammad , Crane Rebecca S. , Almoula Aroub 
Published: January 2023

BackgroundThe outbreak of coronavirus disease 2019 (COVID-19) created unprecedented stress on physicians. Mindfulness is a type of meditation that focuses on being fully present, aware of senses, and emotions in the present moment without analyzing or judging them, and it may help reduce psychological distress in physicians. This study aimed to examine the effectiveness of virtual mindfulness-based intervention (MBI) on physicians’ perceived anxiety and depression and different facets of mindfulness.MethodsDuring the COVID-19 pandemic, an online survey was administered to physicians to assess depression, anxiety, and awareness using the 9-item Patient Health Questionnaire (PHQ-9), 7-item General Anxiety Disorder (GAD-7), and Five-Facets Mindfulness Questionnaire (FFMQ), respectively. Physicians that received the virtual MBI sessions also completed post-questionnaires at a 3-week follow-up time point.ResultsA total of 125 physicians responded to the online survey, with 56 completing the MBI. The prevalence of moderate to severe anxiety and depression was 45.0 and 46.7%, respectively. Mindfulness scores were negatively associated with depression (r = −0.38, P &lt; 0.001) and anxiety (r = −0.36, p &lt; 0.001). Mindfulness scores for the 56 physicians who received virtual MBI sessions were significantly improved (mean difference ± SD, 17.7 ± 16.1, p = 0.001). Significant reductions were also evidenced in anxiety (4.4 ± 4.2) and depression (4.5 ± 5.1) scores (p’s &lt; 0.001). There was also an improvement in mindfulness facets of observing (5.1 ± 4.7), describing (2.3 ± 4.3), acting with awareness (2.7 ± 5.3), non-judging of inner experience (3.6 ± 6.1), and non-reactivity to inner experience (3.9 ± 4.0) (p’s &lt; 0.001). A facet of mindfulness, acting with awareness was most efficiently associated with improved anxiety (B = −0.3, p = 0.02) and depression (B = −0.4, p = 0.01).ConclusionThis study has demonstrated that virtual MBI improved physicians’ psychological wellbeing and mindfulness during the crisis. Regular mindfulness practice may help physicians to tolerate and handle unpleasant circumstances, such as future epidemics or pandemics.