bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluid Influx Promotes Local Strengthening of the Creeping Xianshuihe Fault, Eastern Tibet
Authors: Wu Qiong , Li Haibing , Chevalier Marie‐Luce , Si Jialiang , Pan Jiawei , Li Chunrui , Li Yalin 
Published: June 2023

AbstractWhile the Xianshuihe fault displays continuous creeping behavior, it is also the most seismically active fault in the eastern Tibetan Plateau, and its earthquake mechanisms remain unclear. Here, we aim at the stick‐slip portion of the creeping Qianning segment of the Xianshuihe fault to determine the characteristics of fault rocks and how fluids at depth influence fault behavior. Field survey, optical and scanning electron microscope observations, X‐ray diffraction and fluorescence analyses, as well as carbon and oxygen isotope analyses were performed on the collected samples. The fault core consists of 3–5 cm‐thick black fault gouge and ∼2.5 m‐thick breccia, surrounded by ∼12 m damage zone. In contrast to fault breccia (1–8 cm in diameter), the black fault gouge, which represents the principal slip zone of repeated seismic events, contains angular quartz particles (∼10 μm on average) and clays dominated by illite. The fluid‐rock interactions altering silica minerals into illite, and the thermal decomposition of carbonate minerals, passively increase the relative content of quartz and feldspar (total 63%–73%) in the fault gouge. The deeply sourced CO2 (from mantle and metamorphic degassing) within the hydrothermal fluids causes carbonate precipitation in breccias (21%–53%), composed of calcite, dolomite, and aragonite. These fluid‐assisted reactions lead to more abundant strong mineral phases (quartz, feldspar, and carbonates, 64%–87%) than weak clays (12%–36%) within the fault core, and locally strengthen the fault, which inhibits slow release of stress at shallow depth and promotes seismic rupture of the fault.
Predictability of the Western North Pacific Subtropical High and Associated East Asian Monsoon Rainfall in APCC Multi‐Models
Authors: Kim OkYeon , Lim Chang‐Mook 
Published: June 2023

AbstractUsing selected models with better performance in simulating the Western North Pacific Subtropical High (WNPSH) variability can improve predictions for the East Asia Monsoon (EAM) precipitation. The selected model is clearly superior to the EAM rainfall prediction with the responses to sea surface temperature anomalies (SSTA) forcing and coupling in the atmospheric circulation between the East Asian subtropical Jet and WNPSH, which are similar to those in the observations. The findings of this study reveal that the “poor” model for EAM rainfall prediction is highly correlated with SSTA regardless of El Niño‐Southern Oscillation phases. The “poor” model shows the responding circulation pattern to the El Niño persistent SSTA in the central‐eastern Pacific until JJA, which induces more bias in the prediction of WNPSH variability and EAM rainfall. It is also noticeable that the “poor” model exhibits stronger barotropic response between the WNPSH and EASJ compared to observations, and therefore, it induces stronger relation between the EAJS and EAM rainfall. As a result, both atmospheric circulation itself over the EAM region and atmospheric‐ocean interactions are important to enhance the predictability of the WNPSH and EAM rainfall.
Glutamatergic dysfunction, neuroplasticity, and redox status in the peripheral blood of patients with motor conversion disorders (functional movement disorders): a first step towards potential biomarkers discovery
Authors: Demartini Benedetta , Nisticò Veronica , Benayoun Caroline , Cigognini Anna Chiara , Ferrucci Roberta , Vezzoli Alessandra , Dellanoce Cinzia , Gambini Orsola , Priori Alberto , Mrakic-Sposta Simona 
Published: June 2023

AbstractFunctional movement disorders (FMD) are characterized by the presence of neurological symptoms that cannot be explained by typical neurological diseases or other medical conditions. First evidence showed that, compared to healthy controls (CTR), FMD patients presented increased levels of glutamate+glutamine in the anterior cingulate cortex/medial prefrontal cortex, and decreased levels of glutamate in the cerebrospinal fluid, suggesting that a glutamatergic dysfunction might play a role in FMD pathophysiology. In this study, 12 FMD patients and 20 CTR were recruited and underwent venous blood sampling and urine collection: levels of glutamate, BDNF, dopamine, oxidative stress, creatinine, neopterin, and uric acid were analyzed. Participants also underwent a psychometric assessment investigating depression, anxiety, and alexithymia. We found that levels of glutamate, BDNF, and dopamine were significantly lower in the blood of FMD patients than CTR. Glutamate and dopamine levels were positively associated with levels of alexithymia. Our findings give further evidence that glutamatergic dysfunction might be involved in the pathophysiology of FMD, possibly representing a biomarker of disease; moreover, since glutamatergic and dopaminergic systems are closely interconnected, our results might have a relevance in terms of treatment options for FMD patients.
Deceptive measures of progress in the NHS long-term plan for cancer: case-based vs. population-based measures
Authors: Oke Jason L. , Brown Sarah Jo , Senger Chris , Welch H. Gilbert 
Published: June 2023

AbstractThe NHS Long Term Plan for cancer aims to increase early-stage diagnoses from 50% to 75% and to have 55,000 more people each year survive their cancer for at least 5 years following diagnosis. The targets measures are flawed and could be met without improving outcomes that really matter to patients. The proportion of early-stage diagnoses could increase, while the number of patients presenting at a late-stage remains the same. More patients could survive their cancer for longer, but lead time and overdiagnosis bias make it impossible to know whether anyone had their life prolonged. The target measures should switch from biased case-based measures to unbiased population-based measures that reflect the key objectives in cancer care: reducing late-stage incidence and mortality.
FOXO1 regulates Th17 cell-mediated hepatocellular carcinoma recurrence after hepatic ischemia-reperfusion injury
Authors: Ren Haozhen , Chen Yuyan , Zhu Zhengyi , Xia Jinkun , Liu Shujun , Hu Yingzhe , Qin Xueqian , Zhang Lu , Ding Yitao , Xia Senzhe , Wang Jinglin 
Published: June 2023

Abstract
Background
Hepatic ischemia-reperfusion injury (IRI) is considered as an effecting factor for hepatocellular carcinoma (HCC) recurrence. Th17/Treg cells are a pair of essential components in adaptive immune response in liver IRI, and forkhead box O1 (FOXO1) has the properties of maintaining the function and phenotype of immune cells. Herein, we illuminated the correlation and function between Th17/Treg cell balance and FOXO1 in IRI-induced HCC recurrence.

Methods
RNA sequencing was performed on naive CD4+ T cells from normal and IRI model mice to identify relevant transcription factors. Western blotting, qRT-PCR, immunohistochemical staining, and flow cytometry were performed in IRI models to indicate the effect of FOXO1 on the polarization of Th17/Treg cells. Then, transwell assay of HCC cell migration and invasion, clone formation, wound healing assay, and Th17 cells adoptively transfer was utilized to assess the function of Th17 cells in IRI-induced HCC recurrence in vitro and in vivo.

Results
Owning to the application of RNA sequencing, FOXO1 was screened and assumed to perform a significant function in hepatic IRI. The IRI model demonstrated that up-regulation of FOXO1 alleviated IR stress by attenuating inflammatory stress, maintaining microenvironment homeostasis, and reducing the polarization of Th17 cells. Mechanistically, Th17 cells accelerated IRI-induced HCC recurrence by shaping the hepatic pre-metastasis microenvironment, activating the EMT program, promoting cancer stemness and angiogenesis, while the upregulation of FOXO1 can stabilize the liver microenvironment homeostasis and alleviate the negative effects of Th17 cells. Moreover, the adoptive transfer of Th17 cells in vivo revealed its inducing function in IRI-induced HCC recurrence.

Conclusions
These results indicated that FOXO1-Th17/Treg axis exerts a crucial role in IRI-mediated immunologic derangement and HCC recurrence, which could be a promising target for reducing the HCC recurrence after hepatectomy.
Strain and crystallographic identification of the helically concaved gap surfaces of chiral nanoparticles
Authors: Choi Sungwook , Im Sang Won , Huh Ji-Hyeok , Kim Sungwon , Kim Jaeseung , Lim Yae-Chan , Kim Ryeong Myeong , Han Jeong Hyun , Kim Hyeohn , Sprung Michael , Lee Su Yong , Cha Wonsuk , Harder Ross , Lee Seungwoo , Nam Ki Tae , Kim Hyunjung 
Published: June 2023

AbstractIdentifying the three-dimensional (3D) crystal plane and strain-field distributions of nanocrystals is essential for optical, catalytic, and electronic applications. However, it remains a challenge to image concave surfaces of nanoparticles. Here, we develop a methodology for visualizing the 3D information of chiral gold nanoparticles ≈ 200 nm in size with concave gap structures by Bragg coherent X-ray diffraction imaging. The distribution of the high-Miller-index planes constituting the concave chiral gap is precisely determined. The highly strained region adjacent to the chiral gaps is resolved, which was correlated to the 432-symmetric morphology of the nanoparticles and its corresponding plasmonic properties are numerically predicted from the atomically defined structures. This approach can serve as a comprehensive characterization platform for visualizing the 3D crystallographic and strain distributions of nanoparticles with a few hundred nanometers, especially for applications where structural complexity and local heterogeneity are major determinants, as exemplified in plasmonics.
Examining protective effects of SARS-CoV-2 neutralizing antibodies after vaccination or monoclonal antibody administration
Authors: Follmann Dean , O’Brien Meagan P. , Fintzi Jonathan , Fay Michael P. , Montefiori David , Mateja Allyson , Herman Gary A. , Hooper Andrea T. , Turner Kenneth C. , Chan Kuo- Chen , Forleo-Neto Eduardo , Isa Flonza , Baden Lindsey R. , El Sahly Hana M. , Janes Holly , Doria-Rose Nicole , Miller Jacqueline , Zhou Honghong , Dang Weiping , Benkeser David , Fong Youyi , Gilbert Peter B. , Marovich Mary , Cohen Myron S. 
Published: June 2023

AbstractWhile new vaccines for SARS-CoV-2 are authorized based on neutralizing antibody (nAb) titer against emerging variants of concern, an analogous pathway does not exist for preventative monoclonal antibodies. In this work, nAb titers were assessed as correlates of protection against COVID-19 in the casirivimab + imdevimab monoclonal antibody (mAb) prevention trial (ClinicalTrials.gov #NCT4452318) and in the mRNA-1273 vaccine trial (ClinicalTrials.gov #NCT04470427). In the mAb trial, protective efficacy of 92% (95% confidence interval (CI): 84%, 98%) is associated with a nAb titer of 1000 IU50/ml, with lower efficacy at lower nAb titers. In the vaccine trial, protective efficacies of 93% [95% CI: 91%, 95%] and 97% (95% CI: 95%, 98%) are associated with nAb titers of 100 and 1000 IU50/ml, respectively. These data quantitate a nAb titer correlate of protection for mAbs benchmarked alongside vaccine induced nAb titers and support nAb titer as a surrogate endpoint for authorizing new mAbs.
Morphogenesis of starfish polymersomes
Authors: Sun Jiawei , Kleuskens Sandra , Luan Jiabin , Wang Danni , Zhang Shaohua , Li Wei , Uysal Gizem , Wilson Daniela A. 
Published: June 2023

AbstractThe enhanced membrane stability and chemical versatility of polymeric vesicles have made them promising tools in micro/nanoreactors, drug delivery, cell mimicking, etc. However, shape control over polymersomes remains a challenge and has restricted their full potential. Here we show that local curvature formation on the polymeric membrane can be controlled by applying poly(N-isopropylacrylamide) as a responsive hydrophobic unit, while adding salt ions to modulate the properties of poly(N-isopropylacrylamide) and its interaction with the polymeric membrane. Polymersomes with multiple arms are fabricated, and the number of arms could be tuned by salt concentration. Furthermore, the salt ions are shown to have a thermodynamic effect on the insertion of poly(N-isopropylacrylamide) into the polymeric membrane. This controlled shape transformation can provide evidence for studying the role of salt ions in curvature formation on polymeric membranes and biomembranes. Moreover, potential stimuli-responsive non-spherical polymersomes can be good candidates for various applications, especially in nanomedicine.
Predicting frictional aging from bulk relaxation measurements
Authors: Farain Kasra , Bonn Daniel 
Published: June 2023

AbstractThe coefficient of static friction between solids normally increases with the time they have remained in static contact before the measurement. This phenomenon, known as frictional aging, is at the origin of the difference between static and dynamic friction coefficients but has remained difficult to understand. It is usually attributed to a slow expansion of the area of atomic contact as the interface changes under pressure. This is however challenging to quantify as surfaces have roughness at all length scales. In addition, friction is not always proportional to the contact area. Here we show that the normalized stress relaxation of the surface asperities during frictional contact with a hard substrate is the same as that of the bulk material, regardless of the asperities’ size or degree of compression. This result enables us to predict the frictional aging of rough interfaces based on the bulk material properties of two typical polymers: polypropylene and polytetrafluoroethylene.
The inhibition mechanism of the SUR2A-containing KATP channel by a regulatory helix
Authors: Ding Dian , Hou Tianyi , Wei Miao , Wu Jing-Xiang , Chen Lei 
Published: June 2023

AbstractKATP channels are metabolic sensors for intracellular ATP/ADP ratios, play essential roles in many physiological processes, and are implicated in a spectrum of pathological conditions. SUR2A-containing KATP channels differ from other subtypes in their sensitivity to Mg-ADP activation. However, the underlying structural mechanism remains poorly understood. Here we present a series of cryo-EM structures of SUR2A in the presence of different combinations of Mg-nucleotides and the allosteric inhibitor repaglinide. These structures uncover regulatory helix (R helix) on the NBD1-TMD2 linker, which wedges between NBD1 and NBD2. R helix stabilizes SUR2A in the NBD-separated conformation to inhibit channel activation. The competitive binding of Mg-ADP with Mg-ATP to NBD2 mobilizes the R helix to relieve such inhibition, allowing channel activation. The structures of SUR2B in similar conditions suggest that the C-terminal 42 residues of SUR2B enhance the structural dynamics of NBD2 and facilitate the dissociation of the R helix and the binding of Mg-ADP to NBD2, promoting NBD dimerization and subsequent channel activation.
A corrosion-resistant RuMoNi catalyst for efficient and long-lasting seawater oxidation and anion exchange membrane electrolyzer
Authors: Kang Xin , Yang Fengning , Zhang Zhiyuan , Liu Heming , Ge Shiyu , Hu Shuqi , Li Shaohai , Luo Yuting , Yu Qiangmin , Liu Zhibo , Wang Qiang , Ren Wencai , Sun Chenghua , Cheng Hui-Ming , Liu Bilu 
Published: June 2023

AbstractDirect seawater electrolysis is promising for sustainable hydrogen gas (H2) production. However, the chloride ions in seawater lead to side reactions and corrosion, which result in a low efficiency and poor stability of the electrocatalyst and hinder the use of seawater electrolysis technology. Here we report a corrosion-resistant RuMoNi electrocatalyst, in which the in situ-formed molybdate ions on its surface repel chloride ions. The electrocatalyst works stably for over 3000 h at a high current density of 500 mA cm−2 in alkaline seawater electrolytes. Using the RuMoNi catalyst in an anion exchange membrane electrolyzer, we report an energy conversion efficiency of 77.9% and a current density of 1000 mA cm−2 at 1.72 V. The calculated price per gallon of gasoline equivalent (GGE) of the H2 produced is $ 0.85, which is lower than the 2026 technical target of $ 2.0/GGE set by the United Stated Department of Energy, thus, suggesting practicability of the technology.
The NAD salvage pathway in mesenchymal cells is indispensable for skeletal development in mice
Authors: Warren Aaron , Porter Ryan M. , Reyes-Castro Olivia , Ali Md Mohsin , Marques-Carvalho Adriana , Kim Ha-Neui , Gatrell Landon B. , Schipani Ernestina , Nookaew Intawat , O’Brien Charles A. , Morello Roy , Almeida Maria 
Published: June 2023

AbstractNAD is an essential co-factor for cellular energy metabolism and multiple other processes. Systemic NAD+ deficiency has been implicated in skeletal deformities during development in both humans and mice. NAD levels are maintained by multiple synthetic pathways but which ones are important in bone forming cells is unknown. Here, we generate mice with deletion of Nicotinamide Phosphoribosyltransferase (Nampt), a critical enzyme in the NAD salvage pathway, in all mesenchymal lineage cells of the limbs. At birth, NamptΔPrx1 exhibit dramatic limb shortening due to death of growth plate chondrocytes. Administration of the NAD precursor nicotinamide riboside during pregnancy prevents the majority of in utero defects. Depletion of NAD post-birth also promotes chondrocyte death, preventing further endochondral ossification and joint development. In contrast, osteoblast formation still occurs in knockout mice, in line with distinctly different microenvironments and reliance on redox reactions between chondrocytes and osteoblasts. These findings define a critical role for cell-autonomous NAD homeostasis during endochondral bone formation.
Psychosocial stress, interpersonal sensitivity, and social withdrawal in clinical high risk for psychosis: a systematic review
Authors: Georgiades A. , Almuqrin A. , Rubinic P. , Mouhitzadeh K. , Tognin S. , Mechelli A. 
Published: June 2023

AbstractStress has repeatedly been implicated in the onset and exacerbation of positive symptoms of psychosis. Increasing interest is growing for the role of psychosocial stress in the development of psychosis symptoms in individuals at Clinical High Risk (CHR) for psychosis. A systematic review was therefore conducted to summarize the existing evidence base regarding psychosocial stress, interpersonal sensitivity, and social withdrawal in individuals at CHR for psychosis. An electronic search of Ovid (PsychINFO, EMBASE, MEDLINE, and GLOBAL HEALTH) was conducted until February 2022. Studies that examined psychosocial stress in CHR were included. Twenty-nine studies were eligible for inclusion. Psychosocial stress, interpersonal sensitivity, and social withdrawal were higher in CHR individuals compared to healthy controls and there was some evidence of their association with positive symptoms of psychosis. Two types of psychosocial stressors were found to occur more frequently with CHR status, namely daily stressors, and early and recent trauma, while significant life events did not appear to be significant. Greater exposure to psychosocial stress, emotional abuse, and perceived discrimination significantly increased risk of transition to psychosis in CHR. No studies examined the role of interpersonal sensitivity on transition to psychosis in CHR. This systematic review provides evidence for the association of trauma, daily stressors, social withdrawal, and interpersonal sensitivity with CHR status. Further studies investigating the impact of psychosocial stress on psychosis symptom expression in individuals at CHR and its effects on transition to psychosis are therefore warranted.
Ramularia leaf spot: PCR-based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
Authors: da Silva Aline Suelen , Rennó Marcelo Henrique Lisboa , Quitania Ana Clara Ribeiro , Café-Filho Adalberto Corrêa , Miller Robert Neil Gerard , de Araújo Alderi Emidio , Pinho Danilo Batista 
Published: June 2023

AbstractWhilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated-sequence tree were identical to those in tree generated by RPB2-sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC)4 dendrogram, in identification with species-specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species-specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance.
The QuantuMDx Q-POC SARS-CoV-2 RT-PCR assay for rapid detection of COVID-19 at point-of-care: preliminary evaluation of a novel technology
Authors: Caffry Jessica , Selby Matthew , Barr Katie , Morgan George , McGurk David , Scully Philip , Park Catherine , Caridis Anna-Maria , Southworth Emily , Morrison Jack , Clark David J. , Davies Benedict M. O. , Eckersley Nicholas M. , Groppelli Elisabetta , Kirwan Daniela E. , Monahan Irene , Augustin Yolanda , Toombs Colin , Planche Tim , Staines Henry M. , Krishna Sanjeev 
Published: June 2023

AbstractAccurate and rapid point-of-care (PoC) diagnostics are critical to the control of the COVID-19 pandemic. The current standard for accurate diagnosis of SARS-CoV-2 is laboratory-based reverse transcription polymerase chain reaction (RT-PCR) assays. Here, a preliminary prospective performance evaluation of the QuantuMDx Q-POC SARS-CoV-2 RT-PCR assay is reported. Between November 2020 and March 2021, 49 longitudinal combined nose/throat (NT) swabs from 29 individuals hospitalised with RT-PCR confirmed COVID-19 were obtained at St George’s Hospital, London. In addition, 101 mid-nasal (MN) swabs were obtained from healthy volunteers in June 2021. These samples were used to evaluate the Q-POC SARS-CoV-2 RT-PCR assay. The primary analysis was to compare the sensitivity and specificity of the Q-POC test against a reference laboratory-based RT-PCR assay. The overall sensitivity of the Q-POC test compared with the reference test was 96.88% (83.78– 99.92% CI) for a cycle threshold (Ct) cut-off value for the reference test of 35 and 80.00% (64.35–90.95% CI) without altering the reference test’s Ct cut-off value of 40. The Q-POC test is a sensitive, specific and rapid PoC test for SARS-CoV-2 at a reference Ct cut-off value of 35. The Q-POC test provides an accurate option for RT-PCR at PoC without the need for sample pre-processing and laboratory handling, enabling rapid diagnosis and clinical triage in acute care and other settings.