bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
Authors: Samson Maxime , Genet Coraline , Corbera-Bellalta Marc , Greigert Hélène , Espígol-Frigolé Georgina , Gérard Claire , Cladière Claudie , Alba-Rovira Roser , Ciudad Marion , Gabrielle Pierre-Henry , Creuzot-Garcher Catherine , Tarris Georges , Martin Laurent , Saas Philippe , Audia Sylvain , Bonnotte Bernard , Cid Maria C. 
Published: February 2023

IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment.
Probiotic has prophylactic effect on spatial memory deficits by modulating gut microbiota characterized by the inhibitory growth of Escherichia coli
Authors: Zhang Jie , He Zengyang , Liu Lulu , Li Huailong , Wang Tian , Zhu Xuefeng , Wang Yanqing , Zhu Dongliang , Ning Yong , Xu Yi 
Published: February 2023

Background: The aim of this study is to interrogate the prophylactic effect of probiotic on the lead-induced spatial memory impairment, as well as the underlying mechanisms based on gut microbiota.Methods: Rats were exposed postnatally to 100 ppm of lead acetate during lactation (from postnatal day 1 to 21), to establish the memory deficits model. A probiotic bacterium, namely Lacticaseibacillus rhamnosus, was administered by drinking into pregnant rats with a dosage of 109 CFU/rat/day till birth. At postnatal week 8 (PNW8), the rats were subjected to Morris water maze and Y-maze test, with fecal samples collected for 16S rRNA sequencing. Besides, the inhibitory effect of Lb. rhamnosus on Escherichia coli was carried out in bacterial co-culture.Results: Female rats prenatally exposed to probiotic improved their performances in the behavioral test, indicating that probiotic could protect rats from memory deficits caused by postnatal lead exposure. This bioremediation activity varies depending on the intervention paradigm used. As revealed by microbiome analysis, although administered in a distinct period from lead exposure, Lb. rhamnosus further changed the microbial structure disrupted by lead exposure, suggesting an effective transgenerational intervention. Of note, gut microbiota, represented by Bacteroidota, varied greatly depending on the intervention paradigm as well as the developmental stage. The concerted alterations were revealed between some keystone taxa and behavioral abnormality, including lactobacillus and E. coli. To this end, an in vitro co-culture was created to demonstrate that Lb. rhamnosus could inhibit the growth of E. coli with direct contact, which is dependent on the growth condition under study. In addition, in vivo infection of E. coli O157 aggravated memory dysfunction, which could also be rescued by probiotic colonization.Conclusions: Early probiotic intervention could prevent organisms from lead-induced memory decline in later life through reprogramming gut microbiota and inhibiting E. coli, providing a promising approach to ameliorate the cognitive damage with environmental origins.
Healthcare coverage affects survival of EGFR-mutant Thai lung cancer patients
Authors: Khiewngam Khantong , Oranratnachai Songporn , Kamprerasart Kaettipong , Kunakorntham Patratorn , Sanvarinda Pimtip , Trachu Narumol , Pimsa Pongput , Wiwitkeyoonwong Jirapath , Thamrongjirapat Thanaporn , Dejthevaporn Thitiya , Sirachainan Ekaphop , Reungwetwattana Thanyanan 
Published: February 2023

BackgroundDespite significant benefits of epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) treatment in patients with EGFR-mutated NSCLC, access remains limited in Thailand and elsewhere.MethodsRetrospective analysis of patients with locally advanced/recurrent NSCLC and known EGFR mutation (EGFRm) status treated at Ramathibodi Hospital (2012–2017). Prognostic factors for overall survival (OS), including treatment type and healthcare coverage, were analyzed using Cox regression.ResultsOf 750 patients, 56.3% were EGFRm-positive. After first-line therapy (n=646), 29.4% received no subsequent (second-line) treatment. EGFR-TKI-treated EGFRm-positive patients survived significantly longer than EGFRm-negative patients without EGFR-TKIs (median OS [mOS] 36.4 vs. 11.9 months; hazard ratio HR=0.38 [95%CI 0.32–0.46], P&lt;0.001). Cox regression indicated significantly longer OS in patients with comprehensive healthcare coverage that included reimbursement of EGFR-TKIs, versus basic coverage (mOS 27.2 vs. 18.3 months; adjusted HR=0.73 [95%CI 0.59–0.90]). Compared with best supportive care (BSC; reference), EGFR-TKI-treated patients survived significantly longer (mOS 36.5 months; adjusted HR (aHR)=0.26 [95%CI 0.19–0.34]), and versus chemotherapy alone (14.5 months; aHR=0.60 [95%CI 0.47–0.78]). In EGFRm-positive patients (n=422), relative survival benefit of EGFR-TKI treatment remained highly significant (aHR[EGFR-TKI]=0.19 [95%CI 0.12–0.29]; aHR(chemotherapy only)=0.50 [95%CI 0.30–0.85]; reference:BSC), indicating that healthcare coverage (reimbursement) affected treatment choice and survival.ConclusionOur analysis describes EGFRm prevalence and survival benefit of EGFR-TKI therapy for EGFRm-positive NSCLC patients treated from 2012–2017, one of the largest such Thai datasets. Together with research by others, these findings contributed evidence supporting the decision to broaden erlotinib access on healthcare schemes in Thailand from 2021, demonstrating the value of local real-world outcome data for healthcare policy decision-making.
Patient-specific virtual three-dimensional surgical navigation for gastric cancer surgery: A prospective study for preoperative planning and intraoperative guidance
Authors: Park Sung Hyun , Kim Ki-Yoon , Kim Yoo Min , Hyung Woo Jin 
Published: February 2023

IntroductionAbdominal computed tomography (CT) can accurately demonstrate organs and vascular structures around the stomach, and its potential role for image guidance is becoming increasingly established. However, solely using two-dimensional CT images to identify critical anatomical structures is undeniably challenging and not surgeon-friendly. To validate the feasibility of a patient-specific 3-D surgical navigation system for preoperative planning and intraoperative guidance during robotic gastric cancer surgery.Materials and methodsA prospective single-arm open-label observational study was conducted. Thirty participants underwent robotic distal gastrectomy for gastric cancer using a virtual surgical navigation system that provides patient-specific 3-D anatomical information with a pneumoperitoneum model using preoperative CT-angiography. Turnaround time and the accuracy of detecting vascular anatomy with its variations were measured, and perioperative outcomes were compared with a control group after propensity-score matching during the same study period.ResultsAmong 36 registered patients, 6 were excluded from the study. Patient-specific 3-D anatomy reconstruction was successfully implemented without any problems in all 30 patients using preoperative CT. All vessels encountered during gastric cancer surgery were successfully reconstructed, and all vascular origins and variations were identical to operative findings. The operative data and short-term outcomes between the experimental and control group were comparable. The experimental group showed shorter anesthesia time (218.6 min vs. 230.3 min; P=0.299), operative time (177.1 min vs. 193.9 min; P=0.137), and console time (129.3 min vs. 147.4 min; P=0.101) than the control group, although the differences were not statistically significant.ConclusionsPatient-specific 3-D surgical navigation system for robotic gastrectomy for gastric cancer is clinically feasible and applicable with an acceptable turnaround time. This system enables patient-specific preoperative planning and intraoperative navigation by visualizing all the anatomy required for gastrectomy in 3-D models without any error.Clinical trial registrationClinicaltrials.gov, identifier NCT05039333.
Ectopic scrotum and penoscrotal transposition: Case report and literature review
Authors: Huang Haoran , Liu Xiangxia , Li Zuoqing , Lin Junjie , Yang Hai , Xu Zhe 
Published: February 2023

BackgroundEctopic scrotum (ES) is an extremely rare congenital scrotal malformation. Ectopic scrotum with VATER/VACTERL [vertebral defects (V), anal atresia or anorectal malformations (A), cardiac defects (C), tracheoesophageal fistula with or without esophageal atresia (TE), cardiac defects, renal malformations (R), and limb defects (L)] association is even rarer. There are no uniform guidelines for diagnosis and treatment.Clinical caseWe described a 2-year-5-month-old boy who has ectopic scrotum and penoscrotal transposition and reviewed relevant literature in this report. We performed laparoscopy exploration, rotation flap scrotoplasty, and orchiopexy and achieved a great result during the postoperative follow-up.ConclusionsCombined with the previous literature, we made a summary to come up with a plan for the diagnosis and treatment of ectopic scrotum. Rotation flap scrotoplasty and orchiopexy are worthy of considering operative methods in treating ES. For penoscrotal transposition or VATER/VACTERL association, we can treat the diseases individually.
Autism spectrum disorder diagnosis using a new panel of immune- and inflammatory-related serum biomarkers: A case-control multicenter study
Authors: Gesundheit Benjamin , Zisman Philip David , Hochbaum Leah , Posen Yehudit , Steinberg Avraham , Friedman Gerald , Ravkin Hersh D. , Rubin Eitan , Faktor Ouriel , Ellis Ronald 
Published: February 2023

Background and objectivesChildren with autism spectrum disorder (ASD) present with distinctive clinical features. No objective laboratory assay has been developed to establish a diagnosis of ASD. Considering the known immunological associations with ASD, immunological biomarkers might enable ASD diagnosis and intervention at an early age when the immature brain has the highest degree of plasticity. This work aimed to identify diagnostic biomarkers discriminating between children with ASD and typically developing (TD) children.MethodsA multicenter, diagnostic case-control study trial was conducted in Israel and Canada between 2014 and 2021. In this trial, a single blood sample was collected from 102 children with ASD as defined in Diagnostic Statistical Manual of Mental Disorders [DSM)-IV (299.00) or DSM-V (299.00)], and from 97 typically developing control children aged 3–12 years. Samples were analyzed using a high-throughput, multiplexed ELISA array which quantifies 1,000 human immune/inflammatory-related proteins. Multiple logistic regression analysis was used to obtain a predictor from these results using 10-fold cross validation.ResultsTwelve biomarkers were identified that provided an overall accuracy of 0.82 ± 0.09 (sensitivity: 0.87 ± 0.08; specificity: 0.77 ± 0.14) in diagnosing ASD with a threshold of 0.5. The resulting model had an area under the curve of 0.86 ± 0.06 (95% CI: 0.811–0.889). Of the 102 ASD children included in the study, 13% were negative for this signature. Most of the markers included in all models have been reported to be associated with ASD and/or autoimmune diseases.ConclusionThe identified biomarkers may serve as the basis of an objective assay for early and accurate diagnosis of ASD. In addition, the markers may shed light on ASD etiology and pathogenesis. It should be noted that this was only a pilot, case-control diagnostic study, with a high risk of bias. The findings should be validated in larger prospective cohorts of consecutive children suspected of ASD.
Companion robots to mitigate loneliness among older adults: Perceptions of benefit and possible deception
Authors: Berridge Clara , Zhou Yuanjin , Robillard Julie M. , Kaye Jeffrey 
Published: February 2023

ObjectiveGiven growing interest in companion robots to mitigate loneliness, large-scale studies are needed to understand peoples’ perspectives on the use of robots to combat loneliness and attendant ethical issues. This study examines opinions about artificial companion (AC) robots regarding deception with dementia and impact on loneliness.MethodsData are from a survey of 825 members of the OHSU Research via Internet Technology and Experience cohort (response rate = 45%). Sixty percent (n = 496) of the age diverse sample (range = 25–88; M = 64; SD = 13.17) is over 64, allowing us to compare across age and consider current and future older adults. Ordinal logistic regressions examined relationships between age, health, and other socio-demographic characteristics and perceptions of impact on loneliness and comfort with deception.ResultsMost participants (68.7%) did not think an AC robot would make them feel less lonely and felt somewhat-to-very uncomfortable (69.3%) with the idea of being allowed to believe that an artificial companion is human. In adjusted models, one additional year of age was associated with lower likelihood of perceived benefit of reducing loneliness [Odds Ratio (OR) = 0.98; (0.97–0.99), p = 0.003] and lower comfort with deception [OR = 0.99; (0.97–1.00), p = 0.044]. Being female was associated with lower likelihood of comfort with deception [OR = 0.68; (0.50–0.93), p = 0.014] and high confidence using computers with greater comfort [OR = 2.18; (1.42–3.38), p &lt; 0.001].DiscussionThere was not strong support for AC robots to mitigate loneliness. Most participants were uncomfortable with this form of deception, indicating need for design solutions for those who want to avoid this possibility, as well as greater attentiveness to desirability and comfort across age and gender.
Effects of ambient particulate exposure on blood lipid levels in hypertension inpatients
Authors: Gao Yanfang , Li Chenwei , Huang Lei , Huang Kun , Guo Miao , Zhou Xingye , Zhang Xiaokang 
Published: February 2023

BackgroundWith modernization development, multiple studies of atmospheric particulate matter exposure conducted in China have confirmed adverse cardiovascular health effects. However, there are few studies on the effect of particulate matter on blood lipid levels in patients with cardiovascular disease, especially in southern China. The purpose of this study was to investigate the association between short- and long-term exposure to ambient particulate matter and the levels of blood lipid markers in hypertension inpatients in Ganzhou, China.MethodsData on admission lipid index testing for hypertension inpatients which were divided into those with and without arteriosclerosis disease were extracted from the hospital's big data center from January 1, 2016 to December 31, 2020, and air pollution and meteorology data were acquired from the China urban air quality real time release platform from January 1, 2015 to December 31, 2020 and climatic data center from January 1, 2016 to December 31, 2020, with data integrated according to patient admission dates. A semi-parametric generalized additive model (GAM) was established to calculate the association between ambient particulate matter and blood lipid markers in hypertension inpatients with different exposure time in 1 year.ResultsLong-term exposure to particulate matter was associated with increased Lp(a) in three kinds of people, and with increased TC and decreased HDL-C in total hypertension and hypertension with arteriosclerosis. But particulate matter was associated with increased HDL-C for hypertension inpatients without arteriosclerosis, at the time of exposure in the present study. It is speculated that hypertension inpatients without arteriosclerosis has better statement than hypertension inpatients with arteriosclerosis on human lipid metabolism.ConclusionLong-term exposure to ambient particulate matter is associated with adverse lipid profile changes in hypertension inpatients, especially those with arteriosclerosis. Ambient particulate matter may increase the risk of arteriosclerotic events in hypertensive patients.
Enlarging the convergence ball of Newton's method on Lie groups
Authors: Argyros Ioannis K. , Hilout Saïd 
Published: February 2023

We present a local convergence analysis of Newton's method for approximating a zero of a mapping from a Lie group into its Lie algebra. Using more precise estimates than before [55, 56] and under the same computational cost, we obtain a larger convergence ball and more precise error bounds on the distances involved. Some examples are presented to further validate the theoretical results.
On the interpolation in linear normed spaces using multiple nodes
Authors: Diaconu Adrian 
Published: February 2023

In the papers [2], [3], [4], [6], [7] we indicated a method of extending the notion of interpolation polynomial for the case of a non-linear mapping \(f : X \to Y\) where \(X\) and \(Y\) are linear spaces with special structures. This extension o¤ers the possibility to establish, in this general and abstract case as well, the main properties known in the case of the interpolation of real fonctions. To switch to the case using multiple nodes, case that compulsorily uses the notion of Fréchet differential of the ?rst order as well as of superiour orders, we will point out the de?nition and certain properties of these differentials. On this basis we can present the manner of building an abstract interpolation polynomial with multiple nodes.
Uso de Yarrowia lipolytica, para produção de biomassa, utilizando soro de leite como fonte de carbono
Authors: Bitencourt Thiago Bergler , Barbosa Andréia de Fátima Silva , Da Silva Vanessa Gomes , Kleinert Edmilson José 
Published: February 2023

O soro de leite é o principal resíduo produzido pela indústria de laticínios durante a fabricação de queijos. O qual como matéria-prima, pode conferir à tecnologia alimentar novas potencialidades devido às propriedades funcionais e nutricionais. Mesmo tendo amplo potencial de aproveitamento, o soro ainda é considerado como um efluente. É importante o desenvolvimento de alternativas para viabilizar o aproveitamento desse resíduo agroindustrial, porque ao mesmo tempo em que a transformação do soro em novos produtos diminui o desperdício, pode proporcionar ganhos às indústrias de laticínios. Diante disto, durante a realização do presente estudo foi empregado o método de secagem por liofilização para desidratar o soro e o transformá-lo em soro em pó para facilitar sua estocagem e armazenagem. Posteriormente foi utilizado como fonte de carbono para bioconversão por fermentação submersa com cinco cepas diferentes de microrganismos do grupo das leveduras Yarrowia lipolytica , com meio mineral suplementado composto de 1% uréia (CH4N2O), 0,1% de fosfato de potássio monobásico (KH2PO4) e 0,05% de sulfato de magnésio. O processo de fermentação foi realizado em incubadora com agitação de aproximadamente 80 rpm em intervalo de tempo pré-determinado em 9 dias á 30°C. A avaliação dos resultados realizados durante cada etapa do processo de bioconversão indicam que a levedura Y. lipolytica QU29 apresentou melhor desempenho para o aumento do teor proteico do soro de leite após o bioprocesso, indicando ainda que pode ser utilizado como fonte carbono para a fermentação, tanto as amostras de soro congelado fresco ou liofilizadas, armazenadas por um ano e que mesmo após a estocagem esse resíduo ainda apresenta potencial para uso.
A mediação como instrumento adequado para as práticas de boa governança nos acordos de acionistas de empresas familiares
Authors: Coelho Juliana Espírito Santo , Barreto Leandro de Marzo 
Published: February 2023

O presente trabalho objetiva tratar da solução de conflitos nas empresas familiares, cuja estrutura é moldada não só pelas relações empresarias e de propriedade, mas também pelo aspecto da questão de fundo lastreado nas relações de afeto e familiares. O espaço para solução desses conflitos, para que a atividade empresarial e a gestão dos negócios sejam modernos e atuais, envolve a adoção da mediação como solução adequada para as questões empresarias envolvendo as atividades e sociedades empresárias familiares. Seja em razão de que as práticas de governança atuais exigem a observância da boa-fé, conduta transparente e proba como fundamento do próprio exercício da atividade societária, seja porquanto os aspectos de “lucro a qualquer preço” já não encontram espaço na sociedade pós-moderna, levando a uma revalorização dos negócios familiares pautado em resoluções das divergências de modo célere, adequado aos vínculos relacionais continuados e ético, inerentes aos princípios da mediação.
AKIBAT HUKUM ATAS KETERLAMBATAN AKUISISI SAHAM ANTARA PT. DHARMA SATYA NUSANTARA TBK. TERHADAP PT. AGRO PRATAMA
Authors: Aponno Axcel Deyong , Salsabila Alya , Ponco Nugroho Safari Sidiq , Deborah Hutabarat Sylvana Murni 
Published: February 2023

AbstractAn acquisition is to acquire or buy another company by buying a large portion of the shares of the target company. in Article 29 of Law Number 5 of 1999 in conjunction with Article 6 of Government Regulation Number 57 of 2010. The delay in notifying the takeover of shares is faced by PT. Dharma Satya Nusantara, Tbk, which acquired PT. Agro Pratama. The purpose of this study was to determine the legal consequences of delay in share acquisition between PT. Dharma Satya Nusantara, Tbk with PT Agro Pratama. This research uses empirical normative legal research methods and literature study in collecting the necessary data. The results of the study stated that PT. Dharma Satya Nusantara, Tbk is obliged to pay administrative fines for the delay and the need for further socialization of the company's acquisition process so that cases like this do not happen again. Keywords: Share Acquisition; Business Competition; Legal Consequences AbstrakAkuisisi adalah pengambilalihan atau pembelian perusahaan lain dengan membeli sebagian besar saham perusahaan target. dalam Pasal 29 Undang-Undang Nomor 5 Tahun 1999 jo Pasal 6 Peraturan Pemerintah Nomor 57 Tahun 2010. Keterlambatan pemberitahuan pengambilalihan saham dialami oleh PT. Dharma Satya Nusantara, Tbk yang mengakuisisi PT. Agro Pratama. Tujuan dari penelitian ini adalah untuk mengetahui akibat hukum dari keterlambatan pengambilalihan saham antara PT. Dharma Satya Nusantara, Tbk dengan PT Agro Pratama. Penelitian ini menggunakan metode penelitian hukum normatif empiris dan studi kepustakaan dalam mengumpulkan data-data yang diperlukan. Hasil penelitian menyatakan bahwa PT. Dharma Satya Nusantara, Tbk wajib membayar denda administrasi atas keterlambatan dan perlunya sosialisasi lebih lanjut mengenai proses akuisisi perusahaan agar kasus seperti ini tidak terulang kembali. Keywords: Akuisisi Saham; Persaingan Usaha; Akibat Hukum