bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assay validation for vilanterol and its metabolites in human urine with application for doping control analysis
Authors: Panchal Tina , Baldwin Sandra , Østergaard Martin , Hansen Erik Soeren Halvard , Backer Vibeke , Hostrup Morten , Daley‐Yates Peter 
Published: December 2022

AbstractA bioanalytical method for detecting the ultra‐long‐acting beta2‐agonist (U‐LABA) inhaled vilanterol and its metabolites, GSK932009 and GW630200, in urine was developed to potentially monitor permitted therapeutic versus prohibited supratherapeutic use in sport. The World Anti‐Doping Agency (WADA) has established urinary concentration thresholds for the beta2‐agonists salbutamol and formoterol. Therapeutic use of vilanterol (25 μg once daily) was recently permitted by WADA; however, there is no established decision limit for adverse analytical findings due to insufficient urine concentration data. In this study, we validated an assay to detect vilanterol in urine collected from four healthy male and female athletes 0–72 h who received inhaled corticosteroid fluticasone furoate/U‐LABA vilanterol (800/100 μg) combination, four times the normal therapeutic dose. After administration, subjects performed 1 h of bike ergometer exercise. The experiment was conducted again after repeat dosing for 1 week. Our method utilised liquid chromatography with tandem mass spectrometry and was validated over urine concentrations of 5–5000 (vilanterol) and 50–50,000 pg/ml (GSK932009 and GW630200). Plasma samples were analysed for vilanterol, using a previously validated assay. The peak concentration values for urine vilanterol, GSK932009 and GW630200 were 9.5, 10.4 and 0.17 ng/ml, for single dosing, and 18.6, 19.5 and 0.20 ng/ml, for repeat dosing. Urine samples from four volunteers using the final validated method are reported, demonstrating this assay has sensitivity to detect vilanterol or GSK932009 in urine for ≥72 h post single or repeat dosing with 800/100 μg fluticasone furoate/vilanterol, whereas GW630200 was quantifiable ≤4 h post dose.
Inorganic Chemistry in Hong Kong
Authors: Kam‐Wing Lo Kenneth 
Published: December 2022

AbstractInorganic chemistry research has developed impressively in Hong Kong, attaining worldwide recognition. This Guest Editorial introduces the research showcased in the Institute Feature “Universities in Hong Kong”, which is conducted in seven universities: City University of Hong Kong (CityU), The Chinese University of Hong Kong (CUHK), The Education University of Hong Kong (EdUHK), Hong Kong Baptist University (HKBU), The University of Hong Kong (HKU), The Hong Kong University of Science and Technology (HKUST), and The Hong Kong Polytechnic University (PolyU).
Oropharyngeal dysphagia and cachexia: Intertwined in head and neck cancer
Authors: Willemsen Anna C. H. , Pilz Walmari , Hoeben Ann , Hoebers Frank J. P. , Schols Annemie M. W. J. , Baijens Laura W. J. 
Published: December 2022

AbstractBackgroundThis study aims to investigate the relationship between cancer cachexia and oropharyngeal dysphagia (OD) in patients with head and neck cancer (HNC) prior to chemoradiotherapy or bioradiotherapy (CRT/BRT).MethodsA prospective cohort study with patients with HNC undergoing CRT/BRT (2018–2021) was conducted. Body composition and skeletal muscle function were evaluated using bioelectrical impedance analysis, handgrip strength, and the short physical performance battery (SPPB). The M. D. Anderson Dysphagia Inventory (MDADI), Eating Assessment Tool (EAT)‐10 questionnaire, and patient characteristics were collected. A standardized videofluoroscopic swallowing study was offered to patients.ResultsSixty‐six patients were included. Twenty‐six patients scored EAT‐10 ≥ 3 and seventeen were cachectic. ACE‐27 score >1, cachexia, abnormal SPPB‐derived repeated chair‐stand test, lower MDADI scores, and higher overall stage grouping showed potential predictive value (p ≤ 0.10) for EAT‐10 ≥ 3. Using multivariable regression analysis, only cachexia remained a significant predictor of EAT‐10 ≥ 3 (HR 9.000 [95%CI 2.483–32.619], p = 0.001).ConclusionCachexia independently predicted the presence of patient‐reported OD.
Augmentation of BMP Signaling in Cranial Neural Crest Cells Leads to Premature Cranial Sutures Fusion through Endochondral Ossification in Mice
Authors: Ueharu Hiroki , Pan Haichun , Liu Xia , Ishii Mamoru , Pongetti Jessica , Kulkarni Anshul K. , Adegbenro Folasade E. , Wurn Jaden , Maxson Robert E. , Sun Hongchen , Komatsu Yoshihiro , Zhang Honghao , Yang Jingwen , Mishina Yuji 
Published: December 2022

ABSTRACTCraniosynostosis is a congenital anomaly characterized by the premature fusion of cranial sutures. Sutures are a critical connective tissue that regulates bone growth; their aberrant fusion results in abnormal shapes of the head and face. The molecular and cellular mechanisms have been investigated for a long time, but knowledge gaps remain between genetic mutations and mechanisms of pathogenesis for craniosynostosis. We previously demonstrated that the augmentation of bone morphogenetic protein (BMP) signaling through constitutively active BMP type 1A receptor (caBmpr1a) in neural crest cells (NCCs) caused the development of premature fusion of the anterior frontal suture, leading to craniosynostosis in mice. In this study, we demonstrated that ectopic cartilage forms in sutures prior to premature fusion in caBmpr1a mice. The ectopic cartilage is subsequently replaced by bone nodules leading to premature fusion with similar but unique fusion patterns between two neural crest‐specific transgenic Cre mouse lines, P0‐Cre and Wnt1‐Cre mice, which coincides with patterns of premature fusion in each line. Histologic and molecular analyses suggest that endochondral ossification in the affected sutures. Both in vitro and in vivo observations suggest a greater chondrogenic capacity and reduced osteogenic capability of neural crest progenitor cells in mutant lines. These results suggest that the augmentation of BMP signaling alters the cell fate of cranial NCCs toward a chondrogenic lineage to prompt endochondral ossification to prematurely fuse cranial sutures. By comparing P0‐Cre;caBmpr1a and Wnt1‐Cre;caBmpr1a mice at the stage of neural crest formation, we found more cell death of cranial NCCs in P0‐Cre;caBmpr1a than Wnt1‐Cre;caBmpr1a mice at the developing facial primordia. These findings may provide a platform for understanding why mutations of broadly expressed genes result in the premature fusion of limited sutures. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Collaborative construction of artificial intelligence curriculum in primary schools
Authors: Dai Yun , Liu Ang , Qin Jianjun , Guo Yanmei , Jong Morris Siu‐Yung , Chai Ching‐Sing , Lin Ziyan 
Published: December 2022

AbstractBackgroundThe recent discussion of introducing artificial intelligence (AI) knowledge to K–12 students, like many engineering and technology education topics, has attracted a wide range of stakeholders and resources for school curriculum development. While teachers often have to directly interact with external stakeholders out of the public schooling system, few studies have scrutinized their negotiation process, especially teachers' responses to external influences, in such complex environments.PurposeGuided by an integrated theoretical framework of social constructionism, this research examined the process of how a teacher‐initiated AI curriculum was constructed with external influences. The research focused on teachers' perspectives and responses in mediating external influences into local schools and classrooms.MethodsA 3‐year ethnographic study was conducted in relation to an AI curriculum project among 23 Computer Science (CS) teachers from primary schools. Data collected from ethnographic observation, teacher interviews, and artifacts, were analyzed using open coding and triangulation rooted in the ethnographic, interpretivist approach.ResultsThree sets of external influences were found salient for teachers' curriculum decisions, including the orientation of state‐level educational policies, AI faculty at a partner university, and students' media and technology environments. The teachers' situational logics and strategic actions were reconstructed with thick descriptions to uncover how they navigated and negotiated the external influences to fulfill local challenges and expectations in classrooms and schools.ConclusionsThe ethnographic study uncovered the dynamic and multifaceted negotiation involved in the collaborative curriculum development, and offers insights to inform policymaking, teacher education, and student support in engineering education.
Antennal and proboscis sensilla characteristics of Paranthrene tabaniformis (Lepidoptera: Sesiidae)
Authors: Zheng Yu‐feng , Dong Yan‐ling , Yang Zhao‐fu 
Published: December 2022

AbstractThe poplar clearwing moth, Paranthrene tabaniformis (Lepidoptera: Sesiidae) is a serious wood‐boring pest of several trees. The ultramorphology of the antennae and proboscis sensilla of adult P. tabaniformis was examined using scanning electron microscope to determine their structures and sex‐specific differences. The results showed that the antennae of both sexes are composed of three segments: scape, pedicel and flagellum. Female antennae are clavate while male antennae are pectinate. The number of flagellomeres for females was significantly greater than for males. Seven different types of sensilla were identified on antennae of both males and females: Böhm sensilla, sensilla squamiformia, sensilla trichodea (three subtypes), sensilla chaetica, sensilla coeloconica, and sensilla auricillica (two subtypes), and apical sensors. Three different types of sensilla were found on the proboscis of adult P. tabaniformis: sensilla styloconica, sensilla chaetica, and sensilla basiconica (three subtypes). The sexual dimorphism difference in the number, distributional pattern, the length and the basal width of various sensilla on the antennae and proboscis were determined. This study clarifies the types and sexual dimorphism of the antennal and proboscis sensilla of adult P. tabaniformis and provides useful theoretical foundations for studies on behavioral mechanisms and functions of sensilla of P. tabaniformis.Research HighlightsVarious types of sensilla on the antennae and proboscis of adults Paranthrene tabaniformis were observed. The sexual dimorphism of various sensilla on the antennae and proboscis were determined.
Circadian rhythmicity of amyloid‐beta‐related molecules is disrupted in the choroid plexus of a female Alzheimer's disease mouse model
Authors: Furtado André , Esgalhado André J. , Duarte Ana C. , Costa Ana R. , Costa‐Brito Ana R. , Carro Eva , Ishikawa Hiroshi , Schroten Horst , Schwerk Christian , Gonçalves Isabel , Arosa Fernando A. , Santos Cecília R. A. , Quintela Telma 
Published: December 2022

AbstractThe choroid plexus (CP) is part of the blood‐cerebrospinal fluid barrier (BCSFB) and was recently described as an important component of the circadian clock system. It is the principal source of cerebrospinal fluid (CSF) and responsible for the synthesis and secretion of various neuroprotective peptides including those involved in amyloid‐β (Aβ) transport/degradation, contributing to Aβ homeostasis. Inadequate Aβ metabolic clearance and transport across the BCSFB have been associated with circadian dysfunctions in Alzheimer's disease (AD) patients. To investigate whether AD pathology influences Aβ scavengers circadian expression, we collected CP at different time points from an AD mouse model (APP/PS1) (female and male animals, aged 6‐ and 12‐months‐old) and analyzed their mRNA expression by Real‐time RT‐PCR. Only angiotensin‐converting enzyme (Ace) expression in 6‐month‐old female wild‐type mice and transthyretin (Ttr) expression in 12‐month‐old female wild‐type mice presented significant rhythmicity. The circadian rhythmicity of Ace and Ttr, prompt us to analyze the involvement of circadian rhythm in Aβ uptake. A human CP papilloma (HIBCPP) cell line was incubated with Aβ‐488 and uptake was evaluated at different time points using flow cytometry. Aβ uptake displayed circadian rhythmicity. Our results suggest that AD might affect Aβ scavengers rhythmicity and that Aβ clearance is a rhythmic process possibly regulated by the rhythmic expression of Aβ scavengers.
Comparative histological evaluation of intra‐ and extraorally de‐epithelialized connective tissue graft samples harvested from the posterior palate region
Authors: Couso‐Queiruga Emilio , Gonzalez‐Martin Oscar , Stuhr Sandra , Gamborena Iñaki , Chambrone Leandro , Avila‐Ortiz Gustavo 
Published: December 2022

AbstractBackgroundAutologous connective tissue graft (CTG) is generally considered the gold standard for peri‐implant soft tissue phenotype modification and root coverage therapy. The presence of epithelial remnants in CTG has been associated with complications after soft tissue augmentation surgery. However, a specific method for de‐epithelization that is patently superior has not been identified yet. This study aimed to evaluate the effectiveness of two different approaches to de‐epithelialize CTG samples harvested from the posterior palate.MethodsPatients in need of periodontal or implant‐related surgery that required harvesting a CTG from the posterior palate region were recruited. CTG samples harvested with an indirect approach were de‐epithelialized using either an intraoral (IO group) or an extraoral (EO group) method. Tissue specimens were subsequently processed for histological analysis. The presence or absence of oral epithelial remnants was determined by two examiners using light microscopy.ResultsTwenty‐four patients, who provided a total of 46 analyzable CTG samples, were enrolled in this study. Histological assessment revealed that 19 out of 22 samples in the IO group were free of epithelial remnants. In the EO group, 20 out of 24 samples did not exhibit epithelial residues. These results translate into 86.4% and 83.3% of epithelium‐free samples in the IO and EO groups, respectively.ConclusionsAlthough the intraoral method may provide several practical advantages compared with the extraoral technique, both approaches for de‐epithelialization of CTG samples harvested from the posterior palate region tested in this study were comparably effective. However, none of these methods ensured complete removal of the oral epithelium in a predictable manner, which should be considered in clinical practice.
T4b Sinonasal Squamous Cell Carcinoma: Surgery Plus Radiotherapy May Contribute to Prolonged Survival
Authors: Wang Zekun , Zhang Jianghu , Yang Bining , Zhang Ye , Chen Xuesong , Wang Jingbo , Wu Runye , Wang Kai , Qu Yuan , Huang Xiaodong , Luo Jingwei , Gao Li , Xu Guozhen , Li Ye‐Xiong , Yi Junlin 
Published: December 2022

PurposeTo determine oncologic outcomes for patients with T4b sinonasal squamous cell carcinoma (SNSCC) treated with either surgery plus radiotherapy or definitive radiotherapy.Materials and MethodsBetween January 1999 and December 2016, 85 patients with T4b SNSCC were analyzed retrospectively, there were 54 who received surgery plus radiotherapy (S + RT group) ± chemotherapy and 31 with definitive radiotherapy (RT group) ± chemotherapy. A 1: 2 propensity score matching (PSM) was performed to balance clinical factors and match patients. Kaplan–Meier method and Cox proportional hazard model were used to determine risk factors on survival outcomes.ResultsThe median follow‐up time was 76.7 months. The cumulative rates of locoregional control (LRC), distant metastasis‐free survival (DMFS), cancer‐specific survival (CSS), and overall survival (OS) at 5 years for entire cohort were 44.6%, 33.1%, 38.8%, and 33.9% respectively. After PSM, a total of 50 patients in S + RT group and 25 patients in RT group were analyzed. The 5‐year LRC, DMFS, CSS, and OS between S + RT and RT group were 58.6% versus 27.5% (p = 0.035), 42.8% versus 20.0% (p = 0.006), 50.3% versus 22.0% (p = 0.005), 44.5% veruss 20.0% (p = 0.004). The 5‐year survival rates with orbital retention between groups were 32.7% and 15.0%, p = 0.080. Multivariate Cox analysis revealed non‐surgical therapy (HR = 3.678, 95%CI 1.951–6.933) and invasion of cranial nerves (other than maxillary division of trigeminal nerves) (HR = 2.596, 95%CI 1.217–5.535) were associated with decreased OS.ConclusionThe inclusion of surgery in the multimodal management of T4b SNSCC might confer a survival benefit. Further prospective studies comparing the oncologic outcomes of S + RT with RT are warranted.Level of Evidence3 Laryngoscope, 133:2222–2231, 2023
NPS‐Crosslinked Fibrin Gels Load with EMSCs to Repair Peripheral Nerve Injury in Rats
Authors: Shi Wentao , Wu Yiqing , Wu Jiawei , Gao Yan , Zhao Peng , Lu Xiaojie 
Published: December 2022

AbstractNeural tissue engineering has been introduced as a novel therapeutic strategy for trauma‐induced sciatic nerve defects. Here, a neuropeptide S (NPS)‐crosslinked fibrin scaffolds (NPS@Fg) loaded with an ectomesenchymal stem cell (EMSC) system to bridge an 8‐mm sciatic nerve defect in rats are reported. The Schwann cell‐like and neural differentiation of the EMSCs on the engineered fibrin scaffolds are also assessed in vitro. These results show that the NPS@Fg promotes the differentiation of EMSCs into neuronal lineage cells, which may also contribute to the therapeutic outcome of the NPS@Fg+EMSCs strategy. After transplantation NPS@Fg+EMSCs into sciatic nerve defects in rats, nerve recovery is assessed up to 12 weeks postinjury. In vivo experiments show that the combination of NPS crosslinked fibrin scaffolds with EMSCs can significantly accelerate nerve healing and improve morphological repair. In the study, NPS@Fg+EMSCs may represent a new potential strategy for peripheral nerve reconstruction.
Association between Tomato and Lycopene Consumption and Risk of All‐Cause and Cause‐Specific Mortality: Korean Genome and Epidemiology Study Cohort
Authors: Jung Hyein , Shin Sangah 
Published: December 2022

ScopeTomato has well‐known beneficial health effects and is an important source of lycopene. The study aims to clarify the evidence for the association between tomato and lycopene consumption and all‐cause and cause‐specific mortality in Korean adults.Methods and resultsData analyzed in the current study are obtained from the Korean Genome and Epidemiology Study Cohort. Mortality outcomes are determined based on death records from 2001 to 2020. Tomato and lycopene consumption is assessed using food frequency questionnaires and antioxidant databases and divided into quintiles. Over a mean follow‐up of 11.5 years, 5863 deaths are recorded among 139913 participants aged over 40 years. Tomato consumption is not associated with the risk of all‐cause and cancer mortality. However, the highest tomato consumption group has a lower risk of cardiovascular disease (CVD) mortality (HR = 0.779; 95% CI = 0.633, 0.957). Lycopene consumption is inversely associated with all‐cause (HR = 0.872; 95% CI = 0.801, 0.950), and CVD (HR = 0.680; 95% CI = 0.548, 0.845) mortality.ConclusionA higher intake of tomatoes and lycopene is inversely related to the risk of CVD mortality, and lycopene consumption is associated with a lower risk of all‐cause mortality.
High power balanced TEM horn antenna for ultra wide band radiator
Authors: Singh Sandeep K. , Chandra Romesh , Mitra Sabyasachi , Kumar Ranjeet , Kalyansundaram Senthil , Roy Amitava , Mukherjee Jayanta , Sharma Archana 
Published: December 2022

AbstractA balanced transverse electromagnetic (TEM) horn type antenna is designed and developed for a 120 kV, 66 Ω high power ultra wideband (UWB) radiator. This antenna consists of a coaxial to parallel plate unzipper BALUN followed by an exponentially tapered TEM horn structure. Analysis of this antenna in time and frequency domain is performed using CST Microwave Studio. This antenna is having a power handling capacity of 1000 MW, with percentage bandwidth of 167%. This antenna has a gain of ~10 dB at 1 GHz frequency. Return loss performance of better than −10 dB is observed in 150 MHz to 1 GHz frequency range. Effective radiation of short pulses (<5 ns) with very fast rise time (300–500 ps) has been demonstrated using this high‐power antenna. Practical results at 220 MW power are presented in this article. Directional performance of this antenna is practically established through peak power patterns in horizontal and vertical plane.
Enhancement of antenna decoupling bandwidth by utilizing common and differential modes cancellation
Authors: Wu Wen‐Jing , Cheng Yi‐Feng , Wang Gaofeng 
Published: December 2022

AbstractThis paper proposes a decoupling technique with bandwidth enhanced by introducing two transmission zeros based on common mode (CM) and differential mode (DM) cancellation. The mutual coupling between antennas can be thoroughly eliminated if the CM and DM impedances are equal. Two transmission zeros with closely spaced frequencies are created by using a neutralization line and a parasitic element. The neutral line and parasitic unit can change the currents of radiation patches in CM and DM to realize an enhancement of decoupling bandwidth. To demonstrate the feasibility of this method, a reference and proposed arrays are fabricated and measured. In measurement, the port isolation of proposed antenna array is enhanced by 10.3–21.56 dB in the desired frequency band. Besides, the efficiency and gain are improved by 0.5–3 dB and 10.4%–18%, respectively. The pattern diversity/envelop correlation coefficient (ECC) is also enhanced.
Design of planar vialess baluns with asymmetrical cascaded coupled line sections
Authors: Chang Haojie , Sheng Weixing , Cui Jie 
Published: December 2022

AbstractTwo planar coupled line baluns without vias are designed in this letter. Because of asymmetrical configurations, a virtual port is introduced for even–odd mode analysis, showing the proposed baluns are equivalent to an open‐shorted anti‐coupled line section in odd mode and an open–open coupled line section in even mode. Consequently, bandpass response is obtained in odd mode while suppression is available in even mode, realizing the out‐of‐phase power splitting characteristic. Due to different input and output impedances of the open‐shorted anti‐coupled line sections, the designed baluns have the functionality of impedance transformation, and S‐parameters are then calculated to verify the performance as well. For validation, the two proposed baluns are fabricated with impedance transformers for a 50 Ω test system. The measured results exhibit the two baluns have 16.6% and 18.1% impedance bandwidth centered at 3.5 GHz with an insertion loss of 3.6 and 3.7 dB, respectively.
CGCA 34th annual conference
Authors: Liu Wenlan 
Published: December 2022

AbstractOn the occasion of the 50th anniversary of the establishment of diplomatic relations between China and Germany, the 34th Annual Conference of the Chinese and German Chemical Association (CGCA) was held at the Berlin Technology Center of the Zhejiang Tsinghua Yangtze River Delta Research Institute, in Berlin on the beginning of October. This is the first on‐site gathering of the Chinese chemical colleagues in Germany since the Covid pandemic started. The conference was held in a hybrid form with online participants, and focuses on the topic of “Sustainable Development”. Researchers from Chinese and German top universities, research institutes and well‐known chemical enterprises held ten invited talks and five oral talks. They covered the field of materials, biomedical, and physical chemistry as well as energy chemical industry.