bluuebunny/crossref_metadata_2025_split huggingface dataset:
β3 adrenergic agonism: A novel pathway which improves right ventricular‐pulmonary arterial hemodynamics in pulmonary arterial hypertension
Authors: Karimi Galougahi Keyvan , Zhang Yunjia , Kienzle Vivian , Liu Chia‐Chi , Quek Lake‐Ee , Patel Sanjay , Lau Edmund , Cordina Rachael L. , Figtree Gemma A. , Celermajer David S. 
Published: January 2023

AbstractEfficacy of therapies that target the downstream nitric oxide (NO) pathway in pulmonary arterial hypertension (PAH) depends on the bioavailability of NO. Reduced NO level in PAH is secondary to “uncoupling” of endothelial nitric oxide synthase (eNOS). Stimulation of β3 adrenergic receptors (β3 ARs) may lead to the recoupling of NOS and therefore be beneficial in PAH. We aimed to examine the efficacy of β3 AR agonism as a novel pathway in experimental PAH. In hypoxia (5 weeks) and Sugen hypoxia (hypoxia for 5 weeks + SU5416 injection) models of PAH, we examined the effects of the selective β3 AR agonist CL316243. We measured echocardiographic indices and invasive right ventricular (RV)–pulmonary arterial (PA) hemodynamics and compared CL316243 with riociguat and sildenafil. We assessed treatment effects on RV–PA remodeling, oxidative stress, and eNOS glutathionylation, an oxidative modification that uncouples eNOS. Compared with normoxic mice, RV systolic pressure was increased in the control hypoxic mice (p < 0.0001) and Sugen hypoxic mice (p < 0.0001). CL316243 reduced RV systolic pressure, to a similar degree to riociguat and sildenafil, in both hypoxia (p < 0.0001) and Sugen hypoxia models (p < 0.03). CL316243 reversed pulmonary vascular remodeling, decreased RV afterload, improved RV–PA coupling efficiency and reduced RV stiffness, hypertrophy, and fibrosis. Although all treatments decreased oxidative stress, CL316243 significantly reduced eNOS glutathionylation. β3 AR stimulation improved RV hemodynamics and led to beneficial RV–PA remodeling in experimental models of PAH. β3 AR agonists may be effective therapies in PAH.
Tumorigenesis from non-alcoholic steatohepatitis to hepatocellular carcinoma
Authors: Yu Junjie , Pajvani Utpal B. 
Published: January 2023

Abstract
Non-alcoholic steatohepatitis (NASH) with metabolic syndrome is increasing to be a main cause of hepatocellular carcinoma (HCC). However, the mechanism of tumorigenesis in NASH induced HCC is still not clear. In this perspective, we will discuss the recent progress that has been made to understand the genetic change and the immune microenvironment of HCC, and the remaining questions. Based on the current study, NASH-HCC is likely to have novel mechanism, which needs more investigation in future.
Viral Tracing Confirms Paranigral Ventral Tegmental Area Dopaminergic Inputs to the Interpeduncular Nucleus Where Dopamine Release Encodes Motivated Exploration
Authors: Molas Susanna , Zhao-Shea Rubing , Freels Timothy G. , Tapper Andrew R. 
Published: January 2023

AbstractMidbrain dopaminergic (DAergic) neurons of the ventral tegmental area (VTA) are engaged by rewarding stimuli and encode reward prediction error to update goal-directed learning. However, recent data indicate that VTA DAergic neurons are functionally heterogeneous with emerging roles in aversive signaling, salience, and novelty, based in part on anatomic location and projection, highlighting a need to functionally characterize the repertoire of VTA DAergic efferents in motivated behavior. Previous work identifying a mesointerpeduncular circuit consisting of VTA DAergic neurons projecting to the interpeduncular nucleus (IPN), a midbrain area implicated in aversion, anxiety-like behavior, and familiarity, has recently come into question. To verify the existence of this circuit, we combined presynaptic targeted and retrograde viral tracing in the dopamine transporter-Cre mouse line. Consistent with previous reports, synaptic tracing revealed that axon terminals from the VTA innervate the caudal IPN; whereas, retrograde tracing revealed DAergic VTA neurons, predominantly in the paranigral region, project to the nucleus accumbens shell, as well as the IPN. To test whether functional DAergic neurotransmission exists in the IPN, we expressed the genetically encoded DA sensor, dLight 1.2, in the IPN of C57BL/6J mice and measured IPN DA signalsin vivoduring social and anxiety-like behavior using fiber photometry. We observed an increase in IPN DA signal during social investigation of a novel but not familiar conspecific and during exploration of the anxiogenic open arms of the elevated plus maze. Together, these data confirm VTA DAergic neuron projections to the IPN and implicate this circuit in encoding motivated exploration.
HERC3 promotes YAP/TAZ stability and tumorigenesis independently of its ubiquitin ligase activity
Authors: Yuan Bo , Liu Jinquan , Shi Aiping , Cao Jin , Yu Yi , Zhu Yezhang , Zhang Chengbin , Qiu Yifei , Luo Hongjie , Shi Jiaxian , Cao Xiaolei , Xu Pinglong , Shen Li , Liang Tingbo , Zhao Bin , Feng Xin‐Hua 
Published: January 2023

AbstractYAP/TAZ transcriptional co‐activators play pivotal roles in tumorigenesis. In the Hippo pathway, diverse signals activate the MST‐LATS kinase cascade that leads to YAP/TAZ phosphorylation, and subsequent ubiquitination and proteasomal degradation by SCFβ‐TrCP. When the MST‐LATS kinase cascade is inactive, unphosphorylated or dephosphorylated YAP/TAZ translocate into the nucleus to mediate TEAD‐dependent gene transcription. Hippo signaling‐independent YAP/TAZ activation in human malignancies has also been observed, yet the mechanism remains largely elusive. Here, we report that the ubiquitin E3 ligase HERC3 can promote YAP/TAZ activation independently of its enzymatic activity. HERC3 directly binds to β‐TrCP, blocks its interaction with YAP/TAZ, and thus prevents YAP/TAZ ubiquitination and degradation. Expression levels of HERC3 correlate with YAP/TAZ protein levels and expression of YAP/TAZ target genes in breast tumor cells and tissues. Accordingly, knockdown of HERC3 expression ameliorates tumorigenesis of breast cancer cells. Our results establish HERC3 as a critical regulator of the YAP/TAZ stability and a potential therapeutic target for breast cancer.
Improving Adolescent Violent Trauma Outcomes With a Hospital-Based Violence Prevention Initiative
Authors: Romo Noé D. , Castillo Catherine , Green Jaylen , Lin Juan , Mendelsohn Erika , Dawkins-Hamilton Carjah , Reddy Srinivas H. , Blumberg Stephen M. 
Published: January 2023

BACKGROUND
Violent trauma results in significant morbidity/mortality in Black/Hispanic males aged 15 to 24 years. Hospital- and community-level interventions may improve patient and community outcomes.

OBJECTIVE
To determine if a hospital-based violence prevention intervention using community outreach workers was associated with improved violent trauma patient postdischarge follow-up and reinjury rates.

METHODS
This is a retrospective, single-center, cohort study of admitted violent trauma patients to a public hospital in the Bronx, NY. Data were collected from a convenience sample of patients aged 15 to 24 years admitted with International Classification of Diseases, 10th Revision, codes for gunshot wound, stab wound, or physical assault from August 2014 to April 2018. The exposure variable was documentation of intervention team evaluation during admission. The outcome variables included attending &gt;50% scheduled postdischarge follow-up visits, and subsequent violent reinjury (gunshot wound, stab wound, blunt assault) during the study time period. Multivariable regression models were used to determine the association between the exposure and outcome variables.

RESULTS
A total of 535 patients were evaluated and were primarily male (92.5%), Black (54%)/Latino (36.4%), with mean age of 19.1 years. Patients in the exposure group had increased odds of attending &gt;50% of scheduled clinic postdischarge follow-up visits (odds ratio, 2.29; 95% confidence interval 1.59–3.29) and decreased odds of subsequent violent reinjury presentation (odds ratio, 0.41; 95% confidence interval 0.22–0.75) 3 months after hospital discharge.

CONCLUSION
A hospital-based violence prevention intervention may be associated with decreased odds of violent reinjury and increased odds of postdischarge scheduled appointment adherence in admitted pediatric violent trauma patients.
Analyzing the maxillary sinuses using 3D-modeling
Authors: Zeleva O. V. , Kolsanov A. V. , Zel'ter P. M. , Sidorov E. A. 
Published: January 2023

Introduction. The study of the anatomy of the maxillary sinuses using computed tomography (CT) techniques is an important area of scientific research. The most developing technique that allows you to move from planar images to a picture that clearly represents the shape of the anatomical structure and topographic-anatomical relationships is 3D-modeling.Purpose. Analyze the forms of the maxillary sinuses using 3D-modeling using the Autoplan APK.Materials and methods. The primary analysis included 260 studies, from which 80 studies were subsequently selected. The studies were carried out on Aqulion 32 (Toshiba, Japan) and Revolution EVO 128 (GE, Russia) computed tomographs. MSCT was performed on patients aged 22 to 84 years. The average age of patients in the study group was 52.31±3.18 years. To stratify the patients of the study group by age groups, the age periodization scheme of the Institute of Age Physiology of the Russian Academy of Medical Sciences (1969) was used.Results. The analysis of the shape of the maxillary sinuses showed that the trends in the prevalence of different types of their forms are almost the same, both in the general analysis of the shape of the maxillary sinuses in the study group, and in the isolated analysis of the right and left maxillary sinuses.Conclusion. To conduct a full study of the shape of the maxillary sinuses allows only their three-dimensional modeling, it also allows you to move from linear dimensions to volumetric measurements by highlighting all the elements of the volumetric image that relate to the sinus cavity. The study of the shape of the maxillary sinuses is a modern problem with the lack of a unified approach to the process of segmentality and interpretation of the results. With regard to otorhinolaryngology, three-dimensional reconstructions of images semi-accepted on the basis of computed tomography are effective in assessing the choice of the type of surgery that is most acceptable in a particular patient.
Pre-Visit Digital Messaging Improves Patient-Reported Outcome Measure Participation Prior to the Orthopaedic Ambulatory Visit
Authors: Yedulla Nikhil R. , Hester Joshua D. , Patel Mit M. , Cross Austin G. , Peterson Edward L. , Makhni Eric C. 
Published: January 2023

Background:
Patient-reported outcome measures (PROMs) are metrics that assess physical health, mental health, pain, and satisfaction. However, PROM collection in orthopaedic clinics presents numerous logistical and financial challenges. These challenges are reduced when PROMs are completed before clinic encounters, relieving the workflow constraints of in-office PROM collection. The purpose of this study was to determine the efficacy of 3 different methods with respect to pre-visit electronic PROM completion.

Methods:
Consecutive adult orthopaedic patients with no previous PROM participation were enrolled. Patients who registered with the electronic medical record (EMR) patient portal (MyChart) and with active e-mail addresses were randomly assigned to 1 of 3 arms: control (no pre-visit messages), MyChart (EMR patient portal pre-visit messages), and e-mail (e-mail pre-visit messages). The primary outcome measure was pre-visit PROM completion rates in orthopaedic patients, and the secondary outcome measures were time to pre-visit PROM form completion and PROM form completion rates according to patient demographic characteristics. By default, the Patient-Reported Outcomes Measurement Information System (PROMIS) forms were available for completion through the portal by 7 days before scheduled visits. Pre-visit messages were sent 7 days prior to the scheduled visit except in the control group, with reminders sent 3 days prior if still not completed. The patients in each arm who completed all assigned forms were labeled as having total PROM completion, and those who completed at least 1 completed form were considered as having partial PROM completion. Multivariable logistic regression models were used to assess differences in PROM completion rates between study arms. Kruskal-Wallis tests were performed to compare the date of the form completion.

Results:
A total of 291 patients were included. The pre-visit total completion rates for assigned PROMs were higher in the MyChart arm (49% of 97 patients; p = 0.005) and the e-mail arm (52% of 100 patients; p = 0.002) in comparison with the control arm (30% of 94 patients). Male patients were more likely than female patients to have partial pre-visit PROM completion (odds ratio [OR], 1.74; p = 0.03), and Caucasian patients were more likely to have partial pre-visit PROM completion than African American patients (OR, 2.28; p = 0.01).

Conclusions:
Orthopaedic patients receiving either e-mail or patient portal messages demonstrated higher pre-visit PROM completion rates. Pre-visit messaging appears to be a useful strategy for increasing PROM completion rates and limiting the clinical workflow strain imposed by in-clinic PROM administration.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Establishment of a Novel Rat Model of Gram-Negative Periprosthetic Joint Infection Using Cementless Hip Hemiarthroplasty
Authors: Ibrahim Mazen M. , Liu Yun , Ure Kerstin , Hall Clayton W. , Mah Thien-Fah , Abdelbary Hesham 
Published: January 2023

Background:
Gram-negative periprosthetic joint infections (GN-PJIs) present unique challenges. Our aim was to establish a clinically representative GN-PJI model that recapitulates biofilm formation in vivo. We also hypothesized that biofilm formation on the implant surface would affect its ability to osseointegrate.

Methods:
Three-dimensionally-printed medical-grade titanium hip implants were used to replace the femoral heads of male Sprague-Dawley rats. GN-PJI was induced using 2 bioluminescent Pseudomonas aeruginosa strains: a reference strain (PA14-lux) and a mutant biofilm-defective strain (ΔflgK-lux). Infection was monitored in real time using an in vivo imaging system (IVIS) and magnetic resonance imaging (MRI). Bacterial loads were quantified utilizing the viable colony count. Biofilm formation at the bone-implant interface was visualized using field-emission scanning electron microscopy (FE-SEM). Implant stability, as an outcome, was directly assessed by quantifying osseointegration using microcomputed tomography, and indirectly assessed by identifying gait-pattern changes.

Results:
Bioluminescence detected by the IVIS was focused on the hip region and demonstrated localized infection, with greater ability of PA14-lux to persist in the model compared with the ΔflgK-lux strain, which is defective in biofilm formation. This was corroborated by MRI, as PA14-lux induced relatively larger implant-related abscesses. Biofilm formation at the bone-implant interface induced by PA14-lux was visualized using FE-SEM versus defective-biofilm formation by ΔflgK-lux. Quantitatively, the average viable colony count of the sonicated implants, in colony-forming units/mL, was 3.77 × 108 for PA14-lux versus 3.65 × 103 for ΔflgK-lux, with a 95% confidence interval around the difference of 1.45 × 108 to 6.08 × 108 (p = 0.0025). This difference in the ability to persist in the model was reflected significantly on implant osseointegration, with a mean intersection surface of 4.1 × 106 ± 1.99 × 106 μm2 for PA14-lux versus 6.44 × 106 ± 2.53 × 106 μm2 for ΔflgK-lux and 7.08 × 106 ± 1.55 × 106 μm2 for the noninfected control (p = 0.048).

Conclusions:
To our knowledge, this proposed, novel in vivo biofilm-based model is the most clinically representative for GN-PJI to date, since animals can bear weight on the implant, poor osseointegration was associated with biofilm formation, and localized PJI was assessed by various modalities.

Clinical Relevance:
This model will allow for more reliable testing of novel biofilm-targeting therapeutics.
Youth Empowerment Through Small Medium Micro Business Centers
Authors: Hedar Fitria , Fitria Agustina Isna 
Published: January 2023

Sidoarjo Regency is one of the districts that has potential in the field of home industry economics. One of them is located in Kedung Sumur Village, Krembung Subdistrict, Sidoarjo Regency in the form of a hawker village that has been established since 1961. Kedung Sumur Village Government has tried hard to be able to improve the economy of its people through the Kampung Jajanan UMKM program in hopes of being able to build an independent village in terms of its economy and also able to improve the welfare of its people. Thus the Kedung Sumur Village Government tried to deceive the community including its youth to be directly involved in the Kampung Jajanan UMKM program. The methods used qualitative descriptive data are obtained through observation, documentation, interviews and using the source of books, journals, and policy documents. The results of this study show that youth empowerment in realizing small and medium-sized micro business opportunities in the village of Kedung Sumur Village, Krembung District, Sidoarjo Regency has been running effectively. Seen from the three stages of empowerment that run well, namely the awareness stage in the form of socialization, the stage of ability transformation in the form of training and the stage of improving intellectual ability in the form of product innovation.
Pengaruh Kepemimpinan Islam Terhadap Kinerja Karyawan Melalui Motivasi Kerja Sebagai Variabel Intervening Pada Karyawan BMT Hasanah Ponorogo
Authors: Mukaromah Haniatul 
Published: January 2023

This study aims to analyze the influence of Islamic leadership on employee performance through work motivation as an intervening variable at BMT Hasanah Ponorogo. This research was conducted at BMT Hasanah Ponorogo, and the respondents were employees at the BMT Hasanah Ponorogo. The data type was used as primary data, and the data collection technique used a technical survey with a questionnaire. Analysis of the data in this study using the Submission of a Structural Model (Inner Model) using the SmartPLS analysis tool. Based on the analysis results that have been carried out, the results show that Islamic leadership does not affect employee performance at BMT Hasanah Ponorogo. Islamic leadership positively and significantly affects employee motivation at BMT Hasanah Ponorogo. Motivation positively and significantly affects employee performance at BMT Hasanah Ponorogo. So it can be concluded that to maximize the performance of BMT Hasanah Ponorogo employees, the company must maintain and improve Islamic leadership and work motivation.Penelitian ini bertujuan untuk menganalisis pengaruh kepemimpinan Islam terhadap kinerja karyawan melalui motivasi kerja sebagai variable intervening pada BMT Hasanah Ponorogo. Penelitian ini dilakukan di BMT Hasanah Ponorogo dan yang menjadi responden penelitian ini adalah karyawan BMT Hasanah Ponorogo. Jenis data yang digunakan adalah data primer dan teknik pengumpulan data menggunakan teknis survey dengan alat bantu kuesioner. Analisis data dalam penelitian ini menggunakan Pengajuan Model Struktural (Inner Model) dengan menggunakan alat analisis SmartPLS. Berdasarkan hasil analisis yang telah dilakukan diperoleh hasil bahwa Kepemimpinan Islam tidak berpengaruh terhadap kinerja karyawan pada BMT Hasanah Ponorogo. Kepemimpinan Islam berpengaruh positif dan signifikan terhadap motivasi karyawan pada BMT Hasanah Ponorogo. Motivasi berpengaruh positif dan signifikan terhadap kinerja karyawan pada BMT Hasanah Ponorogo. Sehingga dapat disimpulkan bahwa untuk lebih memaksimalkan kinerja karyawan BMT Hasanah Ponorogo maka perusahaan harus menjaga dan meningkatkan kepemimpinan Islam dan motivasi kerja.
Fuzzy Decision-Making Method based Evaluation of Smart Healthcare Management
Authors: Quasim Mohammad Tabrez , Shaikh Asadullah , Shuaib Mohammed , Sulaiman Adel , Alam Shadab , Asiri Yousef 
Published: January 2023

Abstract
The smart medical system is becoming a health policy service that employs wearables, online services and mobile devices to connect to the internet continuously and connect patients, technology and healthcare centers, and then cope efficiently and thoughtfully with the demands of medical ecosystems. This article explores some of the issues facing users to speed up using and accepting intelligent medical technology for access to omnipresent healthcare. The article analyses how Fuzzy Decision Making may be integrated to create better health solutions with an intelligent approach. The smart medical care management system enables the patient to use medical services and services in any place and at any time, including emergency preparedness, medication management and monitoring services. An assessment of this method for managing new concepts comparable to direct health services must be examined. Hence, this paper Smart Healthcare Management Evaluation utilizing Fuzzy Decision Making (SHME-FDM) model, has been proposed to evaluate the technological integration performance. The present paper assesses the security of health data privacy in the intelligent medicare system utilizing the Fuzzy Analytical Hierarchy Process- Preference Technique similar to the ideal solution (Fuzzy AHP-TOPSIS). This paper usage the fuzzy neural network for health care prediction. The test analyses the reliability, error rate accuracy of the fuzzy outcomes. The security hazard analysis data demonstrate that the suggested fuzzy model has the highest risk assessment performance in contrast to the current models.
Predicting Lung Cancer Survival Prognosis based on the Conditional Survival Bayesian Network
Authors: lu zhong , Yang Fan , Sun Shanshan , Wang Lijie , Yu Hong , Nie Xiushan , Liu Ailing , Xu Ning , Zhang Lanfang , Zhang Mingjuan , Qi Yue , Ji Huaijun , Liu Guiyuan , Zhao Huan , Jiang Yinan , Li Jingyi , Song Chengcun , Yu Xin , Yang Liu , Yu Jinchao , Feng Hu , Guo Xiaolei , Yang Fujun , Xue Fuzhong 
Published: January 2023

Abstract

Lung cancer is one of the leading causes of cancer death and impose an enormous economic burden on patients. It is important to develop an accurate risk assessment model to determine the appropriate treatment for patients after the initial diagnosis of lung cancer. The Cox proportional hazards model is mostly utilized in survival analysis. However, real-world medical data is always incomplete, which poses a great challenge to the application of the Cox proportional hazards model. The commonly used imputation methods cannot achieve sufficient accuracy in the issue of missing data, which drives us to investigate the novel imputation methods for the development of clinical prediction models. In this article, we present a novel missing data imputation method: Bayesian networks for inferring missing covariates. We collected a total of 5,240 patients diagnosed with lung cancer from Weihai Municipal Hospital, China. Then we applied a joint model that combined a Bayesian network and a Cox model to predict mortality risk in individual patients with lung cancer. The established prognostic model achieved a good predictive performance in discrimination and calibration. Through experiments, we proved that the Bayesian network methodology is a robust and accurate approach to addressing the issue of missing data. We showed that combining the Bayesian network with the Cox proportional hazards model is highly beneficial, providing a more efficient tool for risk prediction.