bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome and transcriptome analyses reveal genes involved in the formation of fine ridges on petal epidermal cells in Hibiscus trionum
Authors: Koshimizu Shizuka , Masuda Sachiko , Shibata Arisa , Ishii Takayoshi , Shirasu Ken , Hoshino Atsushi , Arita Masanori 
Published: September 2023

Abstract
Hibiscus trionum, commonly known as the ‘Flower of an Hour’, is an easily cultivated plant in the Malvaceae family that is widespread in tropical and temperate regions, including drylands. The purple base part of its petal exhibits structural colour due to the fine ridges on the epidermal cell surface, and the molecular mechanism of ridge formation has been actively investigated. We performed genome sequencing of H. trionum using a long-read sequencing technology with transcriptome and pathway analyses to identify candidate genes for fine structure formation. The ortholog of AtSHINE1, which is involved in the biosynthesis of cuticular wax in Arabidopsis thaliana, was significantly overexpressed in the iridescent tissue. In addition, orthologs of AtCUS2 and AtCYP77A, which contribute to cutin synthesis, were also overexpressed. Our results provide important insights into the formation of fine ridges on epidermal cells in plants using H. trionum as a model.
Glycogen storage disease with massive left ventricular hypertrophy and increased native T1: a case report
Authors: Wang Jie , Pu Lutong , Han Yuchi , Chen Yucheng 
Published: September 2023

Abstract

Background
Glycogen storage disease (GSD) type Ⅲa is a rare autosomal recessive disorder resulting in the accumulation of abnormally structured glycogen in the liver, skeletal muscle, and cardiac muscle. Cardiovascular magnetic resonance (CMR) tissue characteristics in GSD have rarely been reported.

Case summary
We report a 24-year-old male patient suffering from recurrent palpitation and atypical chest pain for 5 years with suspected hypertrophic cardiomyopathy. Laboratory tests revealed an elevated creatine kinase, and physical exam revealed hepatosplenomegaly. Cardiovascular magnetic resonance demonstrated asymmetrical massive left ventricular hypertrophy with a maximal thickness of 34.6 mm in the septum. In the regions with focal late gadolinium enhancement (LGE) in the anterior septum, both native T1 and extracellular volume (ECV) are elevated. However, in the LGE-negative regions of the myocardium, native T1 was elevated without elevation in ECV (septum, 22.7%; free wall, 20.9%). Whole exome sequencing revealed a novel pathogenic homozygous nonsense variant of the AGL gene (c.4284 T &gt; G, p. Tyr1428*), confirming the diagnosis of the patients as GSD type Ⅲa.

Discussion
This case showed increased diffuse native T1 but not ECV on CMR in LGE-negative myocardium in GSD, which indicates that the T1 value is increased with an accumulation of glycogen in the myocardium, but the ECV space was not expanded in this process. Genetic testing should be obtained in severe LV hypertrophy when multi-organ involvement is present, and myocardial tissue characterization is discrepant between T1 elevation and normal ECV to consider glycogen storage disorder.
Identification of a second genetic alteration in patients withSHOXdeficiency individuals: a potential explanation for phenotype variability
Authors: Dantas Naiara C B , Funari Mariana F A , Lerário Antonio M , Andrade Nathalia L M , Rezende Raíssa C , Cellin Laurana P , Alves Crésio , Crisostomo Lindiane G , Arnhold Ivo J P , Mendonca Berenice , Scalco Renata C , Jorge Alexander A L 
Published: September 2023

AbstractObjectiveOur study aimed to assess the impact of genetic modifiers on the significant variation in phenotype that is observed in individuals with SHOX deficiency, which is the most prevalent monogenic cause of short stature.Design and methodsWe performed a genetic analysis in 98 individuals from 48 families with SHOX deficiency with a target panel designed to capture the entire SHOX genomic region and 114 other genes that modulate growth and/or SHOX action. We prioritized rare potentially deleterious variants.ResultsWe did not identify potential deleterious variants in the promoter or intronic regions of the SHOX genomic locus. In contrast, we found eight heterozygous variants in 11 individuals from nine families in genes with a potential role as genetic modifiers. In addition to a previously described likely pathogenic (LP) variant in CYP26C1 observed in two families, we identified LP variants in PTHLH and ACAN, and variants of uncertain significance in NPR2, RUNX2, and TP53 in more affected individuals from families with SHOX deficiency. Families with a SHOX alteration restricted to the regulatory region had a higher prevalence of a second likely pathogenic variant (27%) than families with an alteration compromising the SHOX coding region (2.9%, P = .04).ConclusionIn conclusion, variants in genes related to the growth plate have a potential role as genetic modifiers of the phenotype in individuals with SHOX deficiency. In individuals with SHOX alterations restricted to the regulatory region, a second alteration could be critical to determine the penetrance and expression of the phenotype.
S2-4 Physical literacy in Denmark: from concept to intervention
Authors: Elsborg Peter , Melby Paulina S , Kurtzhals Mette , Ryom Knud , Nielsen Glen , Bentsen Peter 
Published: September 2023

Abstract

Purpose
Physical literacy (PL) is a holistic concept describing individuals’ prerequisites for engaging in physical activities through the life course. Interest in research on PL has increased rapidly in Denmark in the recent years. Researchers from the three largest universities, the largest university college, as well as research centers at two regional university hospitals have initiated projects with either PL as their core subject or using PL as a guiding theoretical framework for or as an outcome of physical activity interventions. These research projects have a conceptual purpose (Elsborg, Ryom, et al., 2021), a methodological purpose such as development of assessment tools (Elsborg, Melby, et al., 2021), and a purpose of investigating health-related correlates (Elsborg, Heinze, et al., 2021; Melby et al., 2021). Furthermore, interventional research projects that focus on how PL development is best stimulated in different age groups and target groups have recently begun (Ryom et al., 2021; Elsborg et al., 2021). The researchers that have been inspired by PL and are currently conducting PL research in Denmark come mainly from a motivational / sport and exercise psychological and/or public health background and have a strong focus on developing health promotion interventions. Many of the research projects in Denmark are supported by strong collaborations with field leading international researchers.

Methods
Interest and utilization of the concept has also undergone a prodigious development in Denmark from a practice and policy perspective. The national sport for all organisation, DGI, has incorporated PL as one of their key concepts which guides all their activities. The national school sports organisation, Danish School Sports, has also adopted the concept and have currently funded an industrial PhD project, which investigates health-related correlates to PL while also spreading knowledge about the concept in the organisation and in schools. In 2018, a national cross-sectoral network for PL interested parties was established (https://www.pl-net.dk).

Results and discussion
This presentation will focus on providing a short overview and discussion of the research, practice and policy development in Denmark while exemplifying this development by presenting the recent interest from several large-scale school-based intervention projects in more detail.
S4-5 Framework for evaluation of green spaces for physical activity to support public health policy in Slovenija
Authors: Erjavec Ina Šuklje , Kozamernik Jana , Žlender Vita , Koblar Simon 
Published: September 2023

Abstract

Purpose
Evidence suggests that green space use for physical activity significantly benefits to maintenance and improvement of public health. Settlements need to provide adequate access to public green spaces which enable and encourage physical activity. Provision of publicly accessible green spaces is therefore often included in different sectoral strategies and policies. However, to really meet goals and objectives, declared in these documents, appropriate and efficient actions need to be taken in spatial development practice on a local level.

Methods
We present the approach, developed within the first year of the national research project PREZENCA, which aims at developing indicators for the assessment of the provision of settlements with green spaces for outdoor physical activity in Slovenian municipalities. The approach may be of interest to countries which have adequately developed strategic spatial planning of urban green spaces but lack useful tools to assess the current state and to monitor changes in the provision of quality green and other outdoor spaces to encourage physical activity.

Results
The approach is based on a comprehensive analysis of examples and possibilities for measuring presence and quantity of green spaces, defining green space types, and measuring citizens’ accessibility and use of green spaces for physical activity. Content analysis of existing planning documents on a local level was carried out to elaborate: (1) whether the provision of green spaces in settlements in Slovenia is defined and how (2) which population groups are addressed and (3) where such content should be put into policy documents to support implementation in practice. Based on the results, the suggestions on relevant factors and indicators of green space provision and quality for physical activity were prepared to be further developed for evaluation and monitoring on the local level and included into spatial planning practice and policies.

Conclusion
Furthermore, we discuss the importance of establishing local standards and possibilities to include them into planning documents to form the basis for long-term monitoring of the condition of publicly accessible green spaces for recreational use by residents.
S8-1 Health Promoting Sports Federations: theory, tools, case studies and future directions
Authors:   Chair(s):, Van Hoye Aurélie , Geidne Susanna 
Published: September 2023

Abstract

Purpose
Beyond promoting their sports and contributing to elite success, sports federation have a broader health and sport for all promoting role, to include everyone in sport. The symposium has as objective to showcase theoretical grounds, case studies and tools to support health and physical activity promotion among international and national sports federation.

Methods
The symposium will entail four presentation reflecting on different aspects of health and physical activity promotion. The first presentation will focus on theoretical grounds and guidelines created by 15 researchers and in collaboration with the World Health Organisation. The second presentation describes a case study among 51 national sports federation in France, to explain how they promote health and showcase the use of the indicators of health promoting sports federation indicators created by authors of the first presentation. The third presentation will describe a World Athletics project on the promotion of physical activity through competitions organisation and its stakes, as a practice-oriented example. The fourth presentation offer insights on the development and use of the “International and European Sport Organisations Activate Citizens” Capacity Building Framework, created through an Erasmus+ project, which entails a set of tools to empower international sports federation to promote sport for all.

Results
After the four presentation, an open discussion will be engaged with the audience on the success and challenges, as well as a small interactive session on future directions for international and national sports federation to consider their health enhancing physical activity potential and fully exploit it.

Conclusions
The symposium will equip the audience with theory, tools and example of practice, as well as reflect on how to move the research and practice agenda for health promoting sport federation.
S11-1 From physical activity prescription to an active lifestyle: optimising the transfer
Authors:   Chair(s):, Lion Alexis , Füzéki Eszter , McDonough Suzanne , Pfeifer Klaus , Lipkens Luc , Aznar Susana , Titze Sylvia 
Published: September 2023

Abstract

Introduction
Physical activity (PA) is recommended to prevent and manage chronic diseases. Based on a wealth of scientific evidence, the World Health Organization (WHO) published specific PA recommendations for people with chronic diseases. However, people with chronic diseases are still not sufficiently physically active. To tackle this issue, the WHO published a recommendation to “implement systems of patient assessment and counselling on PA in primary and secondary health care and social services”. This symposium will present 4 European examples that (are willing to) implement strategies in healthcare settings to refer patients to PA programs.

Project/policy description
In Germany, an ongoing research project (BewegtVersorgt) aimed to develop, implement, and evaluate a PA referral scheme (PARS) to promote PA for persons with chronic diseases within the German healthcare system. The PARS was coproduced by twelve health sectors organisations under the guidance of a research team.
In Flanders (Belgium), general practitioners (GPs) can use PA Prescription (PAP) since 2017. More recently, a collaboration between different European regions was set up to implement a European Physical Activity on Prescription model (EUPAP), based on the Swedish model. In this model, all healthcare professionals can use PAP. Barriers and facilitators of the implementation of EUPAP in Flanders have been identified.
In Spain, a national PAP plan was developed by the creation of exercise units that will work together with PA referral (PAR) in all autonomic Communities in Spain. Digital tools are used to improve the accessibility and efficacy of the Community Sport and Health systems to provide PA for the inactive population with or without chronic disease.
In Austria, the Jackpot.fit program was developed by Austrian health insurance funds and aims to refer physically inactive adults via several approaches: letter sent by the health insurance company, word of mouth, newspapers, sport-club trainers, and GPs. The letter sent by the health insurance company was much more efficient in terms of patient referral than the other approaches.

Conclusions
These examples will open a discussion between the speakers and the symposium audience and will feed into the reflections of the Working Group on HEPA Promotion in healthcare settings.
S11-2 Co-production of a physical activity referral scheme in the German health care system
Authors: Mino Eriselda , Klamroth Sarah , Weissenfels Anja , Naber Inga , Geidl Wolfgang , Gelius Peter , Abu-Omar Karim , Pfeifer Klaus 
Published: September 2023

Abstract

Purpose
Physical activity referral schemes (PARS) have the potential to promote physical activity (PA) among the mostly inactive primary care population with non-communicable diseases (NCD). Despite significant expansion throughout Europe, a national scheme has not yet been established in Germany. Thus, the ongoing research project BewegtVersorgt aims to develop, implement, and evaluate a PARS to promote PA for persons with NCD within the German healthcare system.

Methods
The PARS was developed using a co-production approach which integrated twelve organizations from different sectors within the healthcare system (health insurances, physicians, exercise professionals, patients’ representatives) and was guided by the research team. Initially, stakeholders’ ideas of a PARS were collected in individual interviews. These were followed by three co-production meetings where individual ideas and current evidence were combined, and different PARS models for the German setting were elaborated. Finally, all stakeholders agreed on one PARS for implementation as a regional model project.

Results
The co-produced PARS starts with the primary care physician, as the gatekeeper in the German healthcare system, who offers 10 min PA advice to the patients who would benefit from PA participation. Afterward, the patients are referred to an exercise professional through a referral form. The exercise professional delivers the remaining central components of the intervention - initial assessment, six 1-hour individual PA counseling sessions spread throughout 12 weeks, final assessment, a progress report to the general practitioner, and one follow-up visit at six months. The intervention uses a patient-centered approach and motivational interviewing. The end goal is an adaptation of an active lifestyle and transfer to local physical activity opportunities.

Conclusions
By using a co-production approach, the expertise of different stakeholders within the healthcare system as well as scientific findings could be integrated into the development process of the PARS. To which extent this participatory approach leads to greater acceptance of the new healthcare service among the implementing actors and positively influences their cooperation within the PARS will be evaluated during the implementation phase.

Support/Funding Source
The study is funded by the Federal Ministry of Health based on a resolution of the German ‘Bundestag’ by the federal government.
S14-5 Girls Active and Change4Life Sports Club - two effective programmes to increase physical activity in schools and colleges
Authors: Wright Chris , Taylor Wendy 
Published: September 2023

Abstract

Purpose
Girls Active aims to tackle declining participation in physical activity of girls aged 10 to 18 and its associated implications on health, wellbeing and education. Change4Life Sports Clubs are designed to address inactivity in 7 to 11 year olds and improve healthy lifestyle behaviours with exercise, food and hydration. Both projects aim to reduce physical inactivity of those children suffering the greatest challenges to achieving the recommended daily physical activity level of an average of 60 minutes of moderate to vigorous physical activity every day.

Project description
Both programmes were developed from evidence reviews and principles that work to engage, inspire and motivate targeted groups of children to be active. They were both developed by the children’s charity Youth Sport Trust, who have used sport and play to improve the lives of children and young people since 1995.
They were co-designed with children and implemented through a national network of 450 School Games Organisers. Youth Sports Trust’s ‘content, delivery, structure’ model was used. For Girls Active we used the Active Lives Children Data from Sport England and Change4Life Sports Clubs were aligned to the Department of Health’s National Child Measurement Programme data to target where the programmes were most needed and to ensure that the right groups of children and young people were included in the interventions.
Both programmes have been externally evaluated through academic partners using pre and post survey data, case studies and control elements and achieved high quality evidence scores. Both programmes have been scaled through a variety of funding streams including London 2012 Olympic Legacy, Sport England Lottery, Corporate Social Responsibility budgets and local public health funding. Girls Active has been adopted in Wales, Scotland and Northern Ireland.

Conclusions
We will share the evidence-based principles that have made these two approaches highly effective in addressing inactivity in primary school aged children and adolescent girls that can be applied in multiple contexts and will share resources and content that can be accessed following the session.
O.2.1-6 Effects of test modality and testing site on exercise-induced hypoalgesia in healthy human males: a protocol
Authors: Aron Vladimir , Deldicque Louise , Mouraux André 
Published: September 2023

Abstract

Background
A single session of physical exercise can acutely reduce experimental pain—a phenomenon known as exercise-induced hypoalgesia (EIH). There is inconsistency in the literature as to (1) whether EIH differentially impact the perception of different types of nociceptive stimuli (e.g., pressure versus thermal stimuli) and (2) whether exercise selectively modulates pain at exercising body parts, or also involves non-exercising body parts.

Purpose
We aim to characterize the effects of a single session of aerobic exercise on the sensitivity to stimuli activating skin versus muscle nociceptors, within or outside exercising body parts.

Methods
We will recruit 40 healthy male participants aged 18-30 years. First, we will familiarize them with the sensory assessments and conduct a maximal cycling test. Then, we will assess, in two sessions, the sensitivity to blunt pressure, mechanical pinprick, contact heat and cold, and auditory stimuli before and after a cycling exercise versus a control session. Sensory testing will be performed over the rectus femoris muscle of the dominant leg (local site) and over the flexor muscles of the non-dominant forearm (remote site). EIH will be quantified as the absolute and relative changes in sensitivity to the sensory stimuli before and after exercise.

Conclusions
We hypothesize that EIH will be greater (1) in the exercise than in the control session, (2) at the local site compared with the remote site if local changes in nociceptive sensitivity contribute to EIH, and (3) when blunt pressure stimuli are used if EIH involves specific changes in sensitivity of muscle nociceptors.
O.4.2-11 Effects of habit formation interventions on physical activity habit strength: meta-analysis and meta-regression
Authors: Ma Haoming , Wang Aoqi , Pei Runyuan , Piao Meihua 
Published: September 2023

Abstract

Background
Interventions aimed at promoting physical activity (PA) behavior through habit formation pathways are gaining popularity, as they differ from conventional interventions that rely on intention pathways. Past research has established a positive correlation between PA habits and behavior. However, the efficacy of current interventions designed to form PA habits and improve PA automaticity is not yet fully ascertained. Additionally, the intervention components that significantly impact the effectiveness of these interventions are yet to be determined.

Methods
We conducted a search of three databases (PubMed, Embase, and Cochrane Library) from January 2000 to December 2022, with a focus on interventions for developing PA habits. Two independent authors conducted paper selection, quality assessment, data extraction, and coding of behavior change techniques (BCTs). The effect size of interventions was calculated using standardized mean difference. Subgroup analyses were carried out based on follow-up duration, delivery method, sample characteristics, and theory. Furthermore, we employed meta-regression to investigate the association between BCTs and PA habits.

Results
Ten eligible studies with relatively high quality were included in the final data set. Characteristics of studies varied in intervention sample and delivery way. The habit formation interventions significantly increased PA habit (SMD=0.31, 95% CI 0.14 - 0.48, P&lt;.001) compared to the control groups. Subgroup analysis demonstrated that the duration of follow-up≤12 weeks have a higher effect size on PA habit than the duration &gt; 12 weeks. Meta-regression revealed that problem solving has a significant positive association with effectiveness improvement (β = 0.36, 95% CI 0.17–0.55), while social reward is linked with a reduction in effectiveness (β = -0.40, 95% CI -0.74–0.06).

Conclusions
Our findings reveal that habit formation interventions are effective in fostering PA habit. Future studies could leverage the insights form this study to optimize the intervention design and achieve better effectiveness.