bluuebunny/crossref_metadata_2025_split huggingface dataset:
The N6‐methyladenosine demethylase FTO is required for odontoblast differentiation in vitro and dentine formation in mice by promoting RUNX2 exon 5 inclusion through RBM4
Authors: Xu Mi , Li Bingrong , Huang Junjun , Jia Rong , Guo Jihua 
Published: September 2023

AbstractAimFat mass and obesity‐associated (FTO) protein, the first discovered N6‐methyladenine (m6A) demethylase, played positive roles in bone formation. In this study, the aim was to investigate the function and potential mechanism of Fto in dentine formation.MethodologyIn vivo model, postnatal 12‐day (PN12), 4‐week‐old (4 wk), 6‐week‐old (6 wk) healthy male C57BL/6J were randomly divided into Fto knockout (Fto−/−) mice and wild‐type (WT) littermates according to their genotypes, with 3–5 mice in each group. The mandibles of Fto−/− mice and WT control littermates were isolated for analysis by micro–computed tomography (micro‐CT), 3‐dimensional reconstruction and Haematoxylin–eosin (HE) staining. In vitro, mouse dental papilla cells (mDPCs) and human dental stem pulp cells (hDPSCs) were cultured with odontogenetic medium to evaluate differentiation capacity; expression levels of odontoblastic related genes were evaluated using quantitative real‐time polymerase chain reaction (qRT‐PCR). The inclusion levels of Runt‐related transcription factor 2 (RUNX2) exon 5 in mDPCs and hDPSCs were detected by semiquantitative real‐time polymerase chain reaction (RT‐PCR). The RNA binding motif protein 4 (RBM4) m6A site was verified through m6A methylated RNA immunoprecipitation (MeRIP) and the stability of RBM4 mRNA influenced by FTO knockdown was measured by mRNA stability assay. Differences with p values < .05 were regarded as statistically significant.ResultsWe discovered that Fto−/− mice showed significant dentine formation defects characterized by widened pulp cavity, enlarged pulp‐tooth volume ratio, thinned dentine and pre‐dentine layer of root (p < .05). Fto−/− mDPCs and FTO‐silencing hDPSCs not only exhibited insufficient mineralization ability and decreased expression levels of odontoblastic mineralization related genes (p < .05), but showed significantly reduced Runx2 exon 5 inclusion level (p < .05). FTO knockdown increased the m6A level of RBM4 and destabilized the mRNA of RBM4, thus contributing to the reduced RBM4 expression level. Moreover, Rbm4 overexpression in Fto−/− mDPCs can partly restore Runx2 exon 5 inclusion level and the differentiation ability disrupted by Fto knockout.ConclusionThus, within the limitations of this study, the data suggest that FTO promotes odontoblastic differentiation during dentine formation by stabilizing RBM4 mRNA to promote RUNX2 exon 5 inclusion.
The steric effect of exfoliation agent for fabrication of layer niobium oxide nanosheet
Authors: Wattanapaphawong Panya , Reubroycharoen Prasert , Samart Chanatip , Kou Lingjiang , Song Jiajia , Su Jinbu , Kajiyoshi Koji 
Published: September 2023

AbstractIn this research, the steric effect of an exfoliation agent between n‐ethylamine and triethylamine for the fabrication of Ca2Nb3O10− nanosheet was investigated. The experimental results revealed that the different structures of the exfoliation agent have affected to fabrication of Ca2Nb3O10− nanosheet. The use of n‐ethylamine as an exfoliation agent gave a maximum exfoliation efficiency of 10 wt.% while the maximum exfoliation efficiency of triethylamine was 90 wt.%. The result showed that the difference in exfoliation efficiency was caused by the steric effect of exfoliation agents. The image of transmission electron microscopy exhibited Ca2Nb3O10− nanosheets which were formed by exfoliation of HCa2Nb3O10. Furthermore, the reaction time for liquid exfoliation was studied in this work. The reaction time at 4 h gave a maximum of exfoliation efficiency. The Ca2Nb3O10− suspension was tested capability to remove hazardous ions. After the experiment, we found the white precipitate which was the formation of CsCa2Nb3O10. The result showed that the Ca2Nb3O10− nanosheet was efficient for the removal of Cs+ ions in the wastewater.
Bio preservation capacity of potentially probiotic Lacticaseibacillus strains in fermented sausage
Authors: Bertuci Marcello Lima , Alves Junior Carlos Alberto , Souza Camila Vespúcio Bis , Penna Ana Lúcia Barretto , da Silva Barretto Andrea Carla 
Published: September 2023

SummaryBio preservation constitutes a well‐acknowledged natural method for controlling the growth of undesirable bacteria in meat products. The aim was to evaluate four different potentially probiotic Lacticaseibacillus strains as bio preservatives and their application in fermented meat sausage. The strains were inoculated for 24 h and used a concentration of 0.1% of the total mass into which it was directly inserted. Regarding the inhibition zone, only the commercial strains exhibited effect on the tested pathogens. They also displayed better growth results on MRS medium, which simulates meat product additives, during the 24‐h period. The pH, sodium nitrite and sodium chloride proved to be limiting factors for the growth of the potentially probiotic Lacticaseibacillus strains. Lc. paracasei BGP1 significantly reduced water activity and pH – both fundamental factors for food conservation‐ when incorporated. Lc. paracasei BGP1 demonstrated its efficacy as bio preservative for use in fermented meat sausage. Technological parameters were evaluated, showing positive responses to the application of acid lactic bacteria. These results indicate the potential of lactic acid bacteria to inhibit certain microorganisms, offering a perspective on inhibition when used in fermented meat products. Hence, further studies could be conducted, primarily concerning Listeria sp.
Conflits du travail dans des contextes de fragmentation et de pluralisme syndical. Analyse empirique du cas du Chili
Authors: PÉREZ AHUMADA Pablo , OCAMPO Gino 
Published: September 2023

RésuméL'incidence des grèves au Chili a considérablement augmenté depuis la fin des années 2000. Dans cet article, nous analysons l'activité de grève en nous concentrant sur un aspect clé du modèle chilien de relations de travail: le pluralisme syndical. Sur la base d'une enquête représentative des entreprises du secteur privé, nous estimons des modèles de régression logistique qui indiquent que le pluralisme syndical augmente significativement la probabilité de grèves dans les entreprises. Cependant, notre analyse ne permet pas de conclure que l'effet positif du pluralisme syndical s'explique par la concurrence entre syndicats, comme le suggère une partie de la littérature internationale.
Isolation drives species gains and losses of insect metacommunities over time in a mountaintop forest archipelago
Authors: da Silva Pedro Giovâni , Beirão Marina do Vale , de Castro Flávio Siqueira , Perillo Lucas Neves , Camarota Flávio , Solar Ricardo R. C. , Fernandes Geraldo Wilson , Neves Frederico de Siqueira 
Published: September 2023

AbstractAimWe evaluated the effects of forest island size, isolation and area in the landscape driving temporal changes of insect biodiversity in a mountaintop forest archipelago. We expected that (i) in smaller, less isolated forest islands, changes in insect composition are more prominent, primarily driven by gains over time; (ii) more forest in the landscape leads to increased gains of vagile species over time, regardless of forest island size and isolation; (iii) less vagile groups undergo heterogenisation, while highly vagile groups experience homogenisation due to differing dispersal capabilities.LocationEspinhaço Range Biosphere Reserve, Brazil.TaxonInsects.MethodsWe used ants, dung beetles, bees, wasps and butterflies as study models to represent a gradient of low‐to‐high dispersal capability. We evaluated the colonisation‐ and extirpation‐resultant components of temporal β‐diversity using area‐ and isolation‐related variables as predictors.ResultsDistinct colonisation‐ and extirpation‐resultant homogenisation and heterogenisation processes are acting according to each insect group, likely due to different dispersal capabilities. Species losses dominated in ants, with widespread and rare species being lost. Butterflies gained species, represented mainly by widespread species, leading to an increased colonisation‐resultant homogenisation. Distance to neighbouring forest islands was the underlying predictor affecting the temporal β‐diversity of insect groups, and also species gains and losses but differently according to the survey period. Effects of the forest amount in the landscape increased the temporal β‐diversity of bees and butterflies but decreased that of ants, dung beetles and wasps.Main ConclusionsThese findings provide valuable biogeographic insights into the complex interactions between forest island characteristics, landscape attributes, and dispersal capabilities that shape the temporal dynamics of insect biodiversity on mountaintops. Conserving the forest amount in the landscape and keeping forest connectivity among forest islands are necessary because the temporal dynamics of local colonisation and extirpation can depend on the organisms' dispersal capability.
The effects of video‐driven discussions integrated into the flipped classroom model on learning achievement, practical performance, and higher‐order thinking skills in dental education
Authors: Fidan Muhammet , Fidan Mustafa 
Published: September 2023

AbstractBackgroundFlipped classroom (FC) model has become increasingly popular in dental education (DE) with its strengths for students. However, major concerns are lack of interaction, unwillingness to complete the assignments, low engagement during the pre‐class activities because of an unwell‐designed instructional setting of FC.ObjectivesThe recent study aims to investigate the effects of video‐driven discussions integrated into the flipped classroom (VdDs‐FC) model on dental students' (DSs) learning achievement, practical performance, and higher‐order thinking skills in a restorative dentistry course.MethodsThis study adopted a sequential explanatory design from mixed‐methods research. A total of 96 DSs participated in a dentistry faculty of Türkiye. They were randomly divided into two groups: VdDs‐FC group and the conventional flipped classroom (c‐FC) group.Results and conclusionsThe results showed that the VdDs‐FC model had significant positive effects on the practical performance of DSs in practical subjects and their problem‐solving and critical thinking skills. However, there was no significant difference between the two groups in learning achievement and creativity. The use of FC in the c‐FC group did not lead to any difference in the students' achievements. Moreover, the qualitative results indicated that the VdDs‐FC model has the potential for enhancing peer learning, practical classroom activities, and higher‐order thinking skills.ImplicationsThis study also includes several instructional implications and suggestions for educators, researchers, and practitioners on use of FC in dentistry.
Inhibitory role of bone marrow mesenchymal stem cells‐derived exosome in non‐small‐cell lung cancer: microRNA‐30b‐5p, EZH2 and PI3K/AKT pathway
Authors: Wu Tong , Tian Qi , Liu Ruiji , Xu Ke , Shi Shanshan , Zhang Xiudi , Gao Liming , Yin Xiaobo , Xu Shufeng , Wang Ping 
Published: September 2023

AbstractExosomal microRNA (miRNA) exerts potential roles in non‐small‐cell lung cancer (NSCLC). The current study elucidated the role of miR‐30b‐5p shuttled by bone marrow mesenchymal stem cells (BMSCs)‐derived exosomes in treating NSCLC. Bioinformatics analysis was performed with NSCLC‐related miRNA microarray GSE169587 and mRNA data GSE74706 obtained for collection of the differentially expressed miRNAs and mRNAs. The relationship between miR‐30b‐5p and EZH2 was predicted and confirmed. Exosomes were isolated from BMSCs and identified. BMSCs‐derived exosomes overexpressing miR‐30b‐5p were used to establish subcutaneous tumorigenesis models to study the effects of miR‐30b‐5p, EZH2 and PI3K/AKT signalling pathway on tumour growth. A total of 86 BMSC‐exo‐miRNAs were differentially expressed in NSCLC. Bioinfomatics analysis found that BMSC‐exo‐miR‐30b‐5p could regulate NSCLC progression by targeting EZH2, which was verified by in vitro cell experiments. Besides, the target genes of miR‐30b‐5p were enriched in PI3K/AKT signalling pathway. Animal experiments validated that BMSC‐exo‐miR‐30b‐5p promoted NSCLC cell apoptosis and prevented tumorigenesis in nude mice via EZH2/PI3K/AKT axis. Collectively, the inhibitory role of BMSC‐derived exosomes‐loaded miR‐30b‐5p in NSCLC was achieved through blocking the EZH2/PI3K/AKT axis.
Effects of endodontic irrigation solutions on structural, chemical, and mechanical properties of coronal dentin: A scoping review
Authors: Marques Joana A. , Falacho Rui I. , Santos João Miguel , Ramos João Carlos , Palma Paulo J. 
Published: September 2023

AbstractObjectiveThis review aims to assess structural, chemical, and mechanical properties of coronal dentin after endodontic irrigation.Materials and MethodsReporting followed the PRISMA extension for scoping reviews. An electronic search was carried out in PubMed, Embase, and Cochrane Library. Records filtered by language and published up to November 4, 2022 were independently screened by two researchers. Studies evaluating structural, chemical, or mechanical properties of human permanent coronal dentin after irrigation within the scope of nonsurgical root canal treatment were included. Data were extracted regarding study type, sample description and size, experimental groups, outcome, evaluation method, and main findings.ResultsFrom the initial 1916 studies, and by adding 2 cross‐references, 11 in vitro studies were included. Seven studies provide ultrastructural and/or chemical characterization, and six assessed microhardness and/or flexural strength. One percent to 8% sodium hypochlorite (NaOCl) and 1%–17% ethylenediaminetetraacetic acid (EDTA) were the most commonly tested solutions, with contact times of 2–240 min (NaOCl) and 1–1440 min (EDTA) being evaluated.ConclusionsOverall, the literature is consensual regarding the inevitable impact of NaOCl and chelating agents on coronal dentin, with both deproteinizing and decalcifying effects being concentration‐ and time‐dependent. The alteration of mechanical parameters further confirmed the surface and subsurface ultrastructural and chemical changes.Clinical SignificanceEndodontic treatment success highly depends on restorative sealing. Understanding the result of exposing coronal dentin, the main substrate for bonding, to irrigants' action is crucial. The deproteinizing and decalcifying effects of NaOCl and chelating agents are both concentration‐ and time‐dependent, causing surface and subsurface ultrastructural, chemical, and mechanical alterations.
Red sea bream iridovirus infection downregulates inflammation‐related genes in the spleen of rock bream (Oplegnathus fasciatus)
Authors: Eurlaphan Chalermkwan , Nozaki Reiko , Sano Motohiko , Koiwai Keiichiro , Hirono Ikuo , Kondo Hidehiro 
Published: September 2023

AbstractThis study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus), a species highly sensitive to the virus. After intraperitoneal injection of the viral solution at 104 TCID50/fish, the viral genome copy number in the spleen was 104.7 ± 0.2 and 105.9 ± 0.4 copies/μg DNA at 3 and 5 days post‐injection (dpi), respectively. Using transcriptomic analyses via MiSeq, viral gene transcripts were detected at 3 and 5 dpi. Six genes including RING‐finger domain‐containing protein and laminin‐type epidermal growth factor‐like domain genes were significantly expressed at 5 dpi. Further, 334 host genes were differentially expressed compared with those before infection. Genes were clustered into four groups based on their expression profiles. Interferon‐stimulated genes were more prevalent in groups showing upregulation at 5 dpi and 3 and 5 dpi. In contrast, the group showing downregulation at 3 dpi included inflammation‐related genes, such as granzyme and eosinophil peroxidase genes. Downregulation of certain inflammation‐related genes may contribute to the susceptibility of this fish to the virus.
Monitoring of water content of apple slices using low‐field nuclear magnetic resonance during drying process
Authors: Guan Yeqing , Hua Zhuqing , Cheng Yudou , He Jingang , Zhang Yang , Guan Junfeng 
Published: September 2023

AbstractIn an aim to examine water content, distribution, and state of apple slices during hot air drying, this study determined the inversion spectra of transverse relaxation time (T2) of apple slices during the drying process at different temperatures using low‐field nuclear magnetic resonance (LF‐NMR) and magnetic resonance imaging (MRI). The results showed that drying temperatures was negatively correlated with water content and positively correlated with drying rate. The relaxation peak area of free water decreased, while those of semi‐bound and bound water increased and subsequently decreased. The water content of apple slices had quadratic polynomial correlations with signal amplitudes of LF‐NMR (peak areas of total, free, and semi‐bound), R2 of these regression fitting equations were basically above 0.8 and 0.9. The models of total and free water had good prediction performance, suggesting that LF‐NMR is suitable for rapid non‐destructive testing of water in apple slices during hot air drying.Practical applicationsIn this work, the amount of water in apple slices at any given time during hot air drying can be predicted well‐using regression fitting equation models of the peak areas of total, free, and semi‐bound water. This information can then be used to improve the quality of dried products and clarify the distribution and states of water during drying. Therefore, LF‐NMR can be used for rapid non‐destructive testing of water in apple slices during hot air drying. And it provided references for further investigation of optimized drying methods and conditions for different apple species and achieved accurate and non‐destructive monitoring of water in fruits during processing based on LF‐NMR.
Banksia (Proteaceae) contains less phylogenetic diversity than expected in Southwestern Australia
Authors: Miller Joseph T. , Prentice Emily , Bui Elisabeth N. , Knerr Nunzio , Mishler Brent D. , Schmidt‐Lebuhn Alexander N. , González‐Orozco Carlos , Laffan Shawn 
Published: September 2023

AbstractBanksia L.f., an iconic genus of the Proteaceae, is endemic to Australia, with its highest species richness and phylogenetic diversity (PD) in southwestern Western Australia. Analysis of the phylogenetic component of richness and endemism uncovered important patterns of Banksia evolutionary history that are not seen at the species level. We found that Banksia is significantly phylogenetically clustered in this region, likely due to recent evolutionary radiations. We also found significant concentrations of phylogenetic endemism in this region, both neoendemism (short, range‐restricted evolutionary branches) and paleoendemism (long, range‐restricted evolutionary branches). There is a striking northwest to southeast divide in phyloturnover in southwestern Western Australia. The majority of the variation in turnover patterns can be explained by environmental factors, with climate representing the largest covariate. This study adds to the growing body of evidence that indicates the importance of integrating phylogenetic and biodiversity data to inform conservation planning.
Metabolic dysfunction‐associated steatotic liver disease in older adults is associated with frailty and social disadvantage
Authors: Clayton‐Chubb Daniel , Kemp William W. , Majeed Ammar , Lubel John S. , Woods Robyn L. , Tran Cammie , Ryan Joanne , Hodge Alexander , Schneider Hans G. , McNeil John J. , Roberts Stuart K. 
Published: September 2023

AbstractBackground & AimsThe burden of metabolic dysfunction‐associated steatotic liver disease (MASLD) is growing rapidly, as is the number of older adults globally. However, relatively few studies have been performed evaluating the prevalence and risk factors for MASLD in older adults. As such, we aimed to identify the prevalence of MASLD in older adults, as well as sociodemographic, clinical, functional and biochemical associations.MethodsThe study population included older adults without a history of cardiovascular disease, dementia or independence‐limiting functional impairment who had participated in the ASPirin in Reducing Events in the Elderly (ASPREE) randomised trial. MASLD was defined using the Fatty Liver Index (FLI). Associations were identified using Poisson regression with robust variance for FLI ≥ 60 vs FLI < 30.Results9097 Australian participants aged ≥70 years had complete biochemical and anthropometric data to identify MASLD. The study population had a mean age of 75.1 ± 4.3 years and was 45.0% male. Almost one‐third (33.0%) had prevalent MASLD, and the prevalence decreased with increasing age (adjusted RR [aRR] 0.96, 95% CI: 0.96–0.97). MASLD was also negatively associated with social advantage (aRR 0.94, 95% CI: 0.90–0.99) and exercise tolerance and was positively associated with diabetes mellitus (aRR: 1.22, 95% CI: 1.16–1.29), hypertension (aRR: 1.31, 95% CI: 1.22–1.41), male sex (aRR: 1.66, 95% CI: 1.57–1.74), pre‐frailty (aRR: 1.99, 95% CI: 1.82–2.12) and frailty (aRR: 2.36, 95% CI: 2.16–2.56). MASLD and nonalcoholic fatty liver disease (NAFLD) results were 100% concordant.ConclusionThis study in a large cohort of relatively healthy community‐dwelling older adults shows that MASLD is common, decreases with age and is associated with poorer metabolic health, social disadvantage and frailty.
National identities, European identity and cosmopolitanism: The case of female golf fans at the 2019 Solheim Cup
Authors: Bairner Alan , Kitching Niamh , Whigham Stuart , Bowes Ali 
Published: September 2023

AbstractThe aim of this paper is to consider the relationship between sport and identity formation by examining testimonies of 24 British women who attended the Solheim Cup golf tournament in 2019 to support a transnational team made up of professional European women players. Relatively little is known about the effects of transnational sports teams on identity formation, not least because such teams are themselves rare. The Solheim Cup, contested by teams of female professional golfers representing the United States and Europe, is one such example. The data set consists of interviews with women who attended the competition that took place in a period of political uncertainty between the UK electorate voting to leave the European Union and Brexit actually taking place. The paper analyses the women's views in relation to national identity, European identity and cosmopolitanism. Although it emerges that most of the women felt more European in the context of a competition involving a team representing Europe and were opposed to the United Kingdom leaving the European Union, they could also be described as cosmopolitan as a result of their educational level, social class and experience of living in or regularly visiting foreign countries.